LOCUS       DAOQUE010000001        18175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_3535_length_18175_cov_4.787583, whole genome shotgun sequence.
ACCESSION   DAOQUE010000001 DAOQUE010000000
VERSION     DAOQUE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18175
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3535_length_18175_cov_4.787583"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PKH10_00005"
     CDS             complement(<1..442)
                     /locus_tag="PKH10_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26533.1"
                     /translation="MKHQLVKLVTIMLFALAFSPLHAVNDDGNAPCFGVHSLGWLDAG
                     FYWREGTTWYKLATNDFSSMYPYPNSGQIYTCYLTADTADKETVSQASVPSVCEPLSS
                     GDKDSCLVFSTKQYAGDAYRYYRVYLGSTSPTDWTYAETWIGTWG"
     gene            complement(618..926)
                     /locus_tag="PKH10_00010"
     CDS             complement(618..926)
                     /locus_tag="PKH10_00010"
                     /inference="COORDINATES: protein motif:HMM:NF020345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HNT26534.1"
                     /translation="MDMQDKGIAITIEDMPNDDLLFVAKNCGVEVAAALMLRLQGLSI
                     SIPRNALRKVIARYICENYNGNNIKQLAVDCRVSIRQVYAILQKNRRLSRSRRPLPGR
                     "
     gene            1101..1466
                     /locus_tag="PKH10_00015"
     CDS             1101..1466
                     /locus_tag="PKH10_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26535.1"
                     /translation="MQGPFYLFRSIVTLVTERRGAGHFRICDKDDRRGLVVRKVGWSD
                     DLQTELLKQAGDRSFNSFFRSFFFEYADSPGEAYERALLDYHEFRGTLGEQEPPRPPT
                     DDPSHRDGRDKNHLSGAGV"
     gene            1463..2332
                     /locus_tag="PKH10_00020"
     CDS             1463..2332
                     /locus_tag="PKH10_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT26536.1"
                     /translation="MNRLCIHLALRDHAFEQKVSAAAQAAGHHTRPVHLDAVINNCRV
                     YDTLFVVEEDYHAILDLIEFHNSCLFNFLPTIVVTTPPSVIDARLSTVRFPYKYLPLE
                     RTLLPSLLPRVARLFDRLRRARSQEDLLRLHGAVVAPFSALGATFGGALDPALRAMLD
                     LLFAQKGSLMLVNRWGNLIVEAATNRAIAGIEVPYDHASPAWSVMESGEPLFCEDILN
                     YPRFKKKLDAYAKDYFLIVPIKLYGKTVGVLNLSDKTVALLFDRADLLRAQGLLRLLE
                     PVLAARCGLLAVR"
     gene            2340..5135
                     /locus_tag="PKH10_00025"
     CDS             2340..5135
                     /locus_tag="PKH10_00025"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT26537.1"
                     /translation="MIGVGVIYHLALLVALSTASGFVSQRWLWRRSGAVLQGLIFGCA
                     AGVGMLAPLALGDGLVFDGRSIVLSLCGLFFGPVAGTSGGGMAVAARLLRGGDDTLLT
                     EILIILLSTVLGALFYLRRSDREAPIGTDRLLFFGLLVHVASLLLLYTLPFDIALLAI
                     KRVGAVFLFAAPLVTALVGRILSDDVSRLRLVEDLRKHKERFRTFFDATADIVTLKDA
                     GGRYLMANRAQCAFLGKKEGDLLGRRDLDLLPEETALRYLRSDQEVLRTKKDMAYHEK
                     VGDRVYEVRKFPVPLREGGLGVGAFVRDITDRERAEGELRESTARSAAILRSVPDLIF
                     LISRDGVFLDYHANDPMLLFAPPQRLLGRHISAVFSPEFAEKLETALAMTLLTDEIRS
                     IEYEEESGETVRCHEMRLVRSGEEAVFMIVRDISDRRRAETDIQEMKELLEERVRERT
                     AQLEEANRRLREARTEAERASKAKSIFLASMSHEIRTPMNAILGYVQLLRRSEPLTGI
                     QREHLSTVLRSGEHLLDLINQILELSKIEAGRITLNPVDLDLPRLLADLRDMFLPQAV
                     EKGLRFEVRQDGAIPRYINADAIKVRQVLINLVGNAMKFTKRGGITLLVRDDGPTFYG
                     NDHLITVEVADTGPGMTPEELSRMFDPFEQTGSGARSGGTGLGLTLSKQFASLMEGTL
                     SARSEPGRGTVVSFSFRALPATAAAATARTRSPMKRLPPGLLYKVLIVDDNASNRDIL
                     RILLTEGGFITHEAKDGREAIAVFWKWRPDLVFMDLQMPEMDGREAIRRIRESPEGKT
                     IPIIVITADPLEETRREMLAAGATGFIRKPFREEELCDAVMKALDIPCDPAPVAEEPR
                     PPAVPPPGVSEAPLRVPDAIRRELHAAVVKGDTGEMLRLLGSLGEGDRPVADRLRALV
                     DDFSYETLLELTEDAHD"
     gene            5122..6216
                     /locus_tag="PKH10_00030"
     CDS             5122..6216
                     /locus_tag="PKH10_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT26538.1"
                     /translation="MPMTERHGVPDIMIVDDTPQNLRLLSELLGAKGYRVRPVPNGAL
                     ALRAAGAEPPDLILLDINMPGMSGFEVCRRLKGTAGLRDIPVLFISALNDTQDKLLAF
                     EAGGQDYITKPFQFEEVLARVNTHLKLERLQRELAWHNRELEDRVAEQVREISESQVA
                     TIVALAKLAESRDDDTGKHIERVQIFCRLLAEKLAESPAFRDRIDPAFVENIFYAAPL
                     HDVGKVGISDSILLKPGKLTPDEFEIMKTHSMIGWRTLQAVCERYPRNAFLSMGRVVA
                     RNHHERWDGNGYPDRLKGDAIPLEARIMAVADVYEALRARRCYKEPFDHEKSRGILAG
                     NGGTQFDPSVIAAFLALDTEFDRIRTDMRD"
     gene            complement(6232..6639)
                     /locus_tag="PKH10_00035"
     CDS             complement(6232..6639)
                     /locus_tag="PKH10_00035"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HNT26539.1"
                     /translation="MTDDITKRDRDDVIFDKRRDTRIGAEFTVSFELAAEGPHKFFTG
                     ITQDISKGGIFLSTLQTLPIGTKMKIAFAVEGRNINAQAEVRWVRGPEASDSMGVTPG
                     MGLRFVDIAEEDRVYIDAYVKEKETIYYDDEEK"
     gene            complement(6782..7075)
                     /locus_tag="PKH10_00040"
     CDS             complement(6782..7075)
                     /locus_tag="PKH10_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNT26540.1"
                     /translation="MNQSVIEEAIRTKAIIEFYYSSHHRVVEPHVLGIHTGIIQLLGY
                     QIDGSSSSGKLPEWRRFDLNRMSNIQITDKKFSGQRPSGHHSSWDRTILVVDD"
     gene            complement(7159..8934)
                     /locus_tag="PKH10_00045"
     CDS             complement(7159..8934)
                     /locus_tag="PKH10_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT26541.1"
                     /translation="MAKKVKAVETAPEVTLEPRPRLSKLIIKNFRCIGASPVTLDLDD
                     IVVLVGPNNVGKSSVLKAYEVIMSEGSNAGHLAIEDFPFGKVDSGAVPEIELHTVIYD
                     NSPGAEWIATTSSGEKLVREQWRWPAPGTPVRRGFNVKENRWATDEDREKVPWGAAGV
                     ANSRRPQPHRVDAFASPDEQAGEIVKLLMMAIKDRVKSHQENSKEDKESDFAKLLGGI
                     AALQKKIVAESEEHIAAVQADLSGYIGRVFPGHRVVFDAKPEDDLDKAINLFKANPQL
                     LMGPEDGFLSSVGRQGSGARRTLLWTAIRLLAETGKKKPEGVQRPHVLLLDEPEICLH
                     PSAVREACDLLYGLPGITGNWQVMVTTHSPCFVDFARDHTSIVRVERREDGQITGTTI
                     FRPERAMFDGTDKECLKLLNLCDPYVAEFFFGGRTIIVEGDTEYTAFKHAIAAAPKKY
                     EGVHVVRARGKATIVSLCKILNQFGAPYSVLHDSDRPTYVKNEKESANPAWALNDSIR
                     IEMAKCPGPTRMVASVPNFEGAYLGEEAKTDKPYNVLSSLKADSAILMTVSELLDALL
                     DFDKPLPHGAVSWKNLDELKASLND"
     gene            complement(9353..9673)
                     /locus_tag="PKH10_00050"
     CDS             complement(9353..9673)
                     /locus_tag="PKH10_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26542.1"
                     /translation="MTMPIETGRAGEKAVIEELKTKRFSIIKWDSLAPGAADIEAAGV
                     GGRKILVQVKASVEPNLPTTLSGEEERGIRSRAAQQDADAYEARVLLDETLQPAGKVR
                     WRKL"
     gene            complement(9685..12309)
                     /locus_tag="PKH10_00055"
     CDS             complement(9685..12309)
                     /locus_tag="PKH10_00055"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNT26543.1"
                     /translation="MNDDTLAFSDDFLASDEDAVPDAEPFWDFRIDRADRDLLAGMLP
                     TADGGVIIAVKNEDTFVGLNGALLIRYNSDLSERWSYCWPDIYVAPIGLAYGPDPSKV
                     IVSGRNSNTFYLAAFNAEEGTLLWERSWKYDWQVALVNSQDELGTLAVGANGIFVTGF
                     SASWPEGGAASYTRIYVAKTDFEGKLIWEKIYGNGGDMGGYIALDGEGNIYITGYTVS
                     GLGDQHNAGKVGDCAGLPFGPAETSSCPDPYLMKLDGEGNMLWARQWGSATANPFDWG
                     VSVVTDVENSVYALSLFAHAQWGDSTIRKYDPDGALLWEYRGATSFYFDRSIRLHLDD
                     SGILWAVGDGGTRKQEARHPELMVFDADTGRRLKKNLYGPLSEEKNAYFSFADMVETN
                     GKLLVAANEMWVSPDTGESFNDLVFKWMDYFYEEPPSYKDEACAGESYTVRQFGTDGE
                     DIPVAVAESHDGTTVMVGNTEGAFPGRSASGLGDIFVTKRKEDGSAAWTVQFGTADND
                     IAAAAVVDGTGNIFVTGHTYGHFAHSWWRHSDPDVFLAKISPDGTVAWIEQMRTEDED
                     YATALAMTGAGNILMFGHTRGSLDGLPKDETECHDKVNADLFLAEWTPKGEVAWLKQW
                     GGDKDDVSAGMTLDAEGSLFITGYTQSELEGNTLIGGLKYFGEPFPGAFASKRTPLGE
                     KVWTQVWQKENGVTDADANETRSGEYLIIKTDGTVVVAGTGAGVFLDPSSEEYTISPC
                     YPSQRCQDIFVSALSPEGILISTRFVGTLLVEELKGMALHEDVVSVFGYTLGVMEDAV
                     SPGGTDCFVTTLGETPKTVQFGTTGEDICRAAVWDEKTSSLIVVGETRNAFKGCANTG
                     NSDAFVVKMPMEITDTKP"
     gene            complement(12504..13277)
                     /locus_tag="PKH10_00060"
     CDS             complement(12504..13277)
                     /locus_tag="PKH10_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26544.1"
                     /translation="MKRIVFLAFMVFAFSGILFAQEISLDEAVKYLEENGYEYEIAYD
                     EAVKELPEGAVVFTPFMVEATKEAAEEALSGGYAALDSTTIQQDWVFGDIWYPQYYRL
                     MMVGVYSVSPIGDLVSYKFMWARNTTSNPLAPVWSNITGSNVCTITAQRYGTLSNCSC
                     SSGEGAYVCTTTTFGEELLNYYSGSSACYQCDHSPYVFSVRFSVNPQSLTWSYNPILK
                     IWIPIYLYLGWVHILSDGDGGEFSPVWDLCETNLYNFCD"
     gene            complement(13456..13821)
                     /locus_tag="PKH10_00065"
     CDS             complement(13456..13821)
                     /locus_tag="PKH10_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNT26545.1"
                     /translation="MNYFYRCPDCRKGISVEWRTRREEIPCPHCKKRHVPPTPREQLE
                     AYVDQREWPQEMEDTVRALKGDLCTVPGCTNHADTLDHRVPWGERGKTSVWNLYPMCR
                     EHNESKGDRDYYEWVAEQI"
     gene            complement(13818..14426)
                     /locus_tag="PKH10_00070"
     CDS             complement(13818..14426)
                     /locus_tag="PKH10_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26546.1"
                     /translation="MTGNNPPCNESGRVMHLDRLFDLFFSGDDPKPISIKGFAHSMIP
                     AILPGELIRILPAGDRTRLRSGDVVVFRYDDIFVAHRLVLTWRGRALTKGDNNPLCDP
                     WISLADIVGVVEGKSSRWRLIRHFIVRERLLPCLWALAQEKWSFLRKADATDSVAVGG
                     DVDPEGSDTSRRPREASSRLLSPAGPSTRPYPQDHLRTGETP"
     gene            complement(14516..16030)
                     /locus_tag="PKH10_00075"
     CDS             complement(14516..16030)
                     /locus_tag="PKH10_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26547.1"
                     /translation="MNKIGAYVSFLLLLVCCLSCSDGKQSEGDTDVSPFDGDFSGDQD
                     GPDNDSLIVPETLDHYSRSKSLTAGSPYNEFVFDVAISTTNGSLLLSGFTEGSFAEYA
                     MKDTMKLSPKCEGRECRDMFLATVSSDIIEPIAQFGWNGETYADDEIASMLVDGNMAI
                     LGGAISAIANCTGLFCQEAVLVGYDYKDKVVNTMLYEMGKGLIPDPIVNVLRPPTRIT
                     SMTHGVFSEIYIAGWVIGDIYGDPGQLTEESYGQDVFVRKIDTATWGNRWVVQLRTPQ
                     NESADAIFLLSDGGIVVAGTTRGDMCAGSLKETVDRDIFFARYDGDGNLLSCRQVLTA
                     EDEIVGAATELNGRIILIGTRRAFDALSKGSVVITEFDPADDTTTSRLLPETTGIKIS
                     AATQMNQKVFLAAGAVEHCVVDDKEKDFPCLVMIQMDDTYTELSRRYFGRKQNERIAK
                     VIVHDDRSISIVGDALTIIPGGKSNRDIWYEKWVVKDQLTARLPATILSPFYSK"
     gene            complement(16023..17225)
                     /locus_tag="PKH10_00080"
     CDS             complement(16023..17225)
                     /locus_tag="PKH10_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26548.1"
                     /translation="MVLGFTSYGEPWNVPNNGDYDIYLVKIDGDGRYAWKESFGTEYS
                     DFLKRLAIGKDNSIWLTGWTQGEFFGHAKSDSVIHPYEDMFVARLDQKGAILFVRQFT
                     RPLALSFAKMFDPVEIVITDDGALLIYNSGAMVFSSDNHDGIGIASLSLTGEITKNVV
                     YEETTLRVPLSANSATSLTNGMIAAAGITDINDNGEDAFVLILDKDAAVVDKLIVSQS
                     GYMMGEALEYATAIISDENDDLYVALSMSYYDEFNGHFPPSGGRGTGADTDAYVVKYD
                     KKLNMLWSTQVASKGSEGISHLFILDDGSLVAVGDTNGDMAVTGEYTGVPYGFWVDID
                     RSTGQVENIEMTPTPQTTINAVLIHGGYRYLAGTTYASFEEQYEDRKTTCDRAGCSDL
                     YLRRELHE"
     gene            complement(17516..>18175)
                     /locus_tag="PKH10_00085"
     CDS             complement(17516..>18175)
                     /locus_tag="PKH10_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26549.1"
                     /translation="VDETIYMSKSFTENDFLNMFYIKNYDSSQKINLDENEYYYFNKN
                     NGHFGWGKKEVESVIPEDETGIIYEFALKESAGLLEKWYDYKMQLESASFETLTGIKD
                     GEPLNEVAIGLHLIYKPAINGKFVDNKKIKMYFTRSGLSKVFSNAPKEIFVSEENVRT
                     KDHETILSEIASQEKMDNMENYEYTGIYYMDETGTIIPAVKYVSDRNILAEKVLIITS
                     D"
BASE COUNT         3867 a         5316 c         4877 g         4115 t
ORIGIN      
        1 gtccccatgt cccgatccag gtctcggcgt aggtccagtc ggtcgggctg gtcgagccga
       61 gatagacgcg gtaataacgg taggcgtcgc ccgcgtactg tttagtggag aagacgagac
      121 acgaatcctt gtcgccactg ctcaacggtt cgcataccga ggggaccgac gcttgggata
      181 cggtctcctt gtcggcggtg tcggccgtca ggtagcaggt gtatatctga ccggaattcg
      241 gatagggata catggaggaa aagtcattcg tagccagttt gtaccaggtc gtcccctccc
      301 gccaataaaa accggcgtcc agccagccga gcgagtggac cccgaaacag ggggcgttgc
      361 cgtcgtcgtt gacggcatgg agcggactga acgcaagcgc gaacagcatg atggtcacaa
      421 gtttaactag ttgatgtttc atgtgcttcc tccttttgca gtctgttttt catcgttcac
      481 ggtcacccgt gggtctcctc gtaggtccac ccctatgata ccggtagggg gaaaaaggat
      541 tctcattatt ttgcattttt gcagttattt tttcacaagg tcgttcaaaa gagggaaaaa
      601 agagggacgc ggcggggtca gcgcccgggg agcggcctcc ggctccggga gaggcgccga
      661 ttcttttgca ggatcgcgta cacctgacgg atactgaccc ggcaatcaac agccaattgc
      721 ttgatattgt tgccgttgta gttttcgcag atatagcgcg cgatgacctt ccgcagggcg
      781 ttccggggga tgctgatgct cagcccctgc aggcgcaaca tcagcgcggc cgccacctcg
      841 accccgcagt tcttcgcgac aaagagcaga tcatcgttgg gcatgtcctc gatagtgatc
      901 gcgatcccct tatcctgcat gtccatgctt tcctccgcct ttctatccgt ccgaccataa
      961 gatacgggag cggagtgaaa agattcttgt cggatgaaaa aaagataaaa aaacggaaat
     1021 agatgttttt ttcagcacag gtgaatcctt tcgtccccgg ccggtatctt ataagcggaa
     1081 cctaggaaaa ggagacgccc atgcaaggac ctttctatct cttccggagc atcgttacgc
     1141 tggtgaccga acggcggggg gccggccatt tccgcatctg cgacaaggac gaccggcgcg
     1201 ggctggtggt gcggaaggtc ggctggtcgg acgacctgca gacggagttg ctcaaacagg
     1261 cgggggaccg atcgttcaat tccttcttcc gctctttttt cttcgaatat gccgattctc
     1321 ccggagaggc ctacgaacgg gccctcctcg actatcatga gttccgggga acgctcggtg
     1381 agcaggaacc gccgcgaccg ccgaccgacg atccgtcgca ccgggatggc cgcgacaaga
     1441 accacctttc cggcgccggg gtatgaaccg cctgtgcata catctcgcgt tgcgcgacca
     1501 cgcgttcgag cagaaggtct ccgcggcggc gcaggccgcc ggccaccata cccgaccggt
     1561 gcatctggac gccgtcatca acaactgccg cgtgtacgac accctcttcg tcgtcgagga
     1621 ggactatcac gcgatcctcg acctcatcga attccacaat tcctgtctgt tcaatttcct
     1681 ccccacgatc gtcgtcacga ccccgccttc cgtcatcgac gcgcggctca gtacggtacg
     1741 gttcccctac aaatacctcc cgctggaacg gacgctcctc ccgtcgctcc tcccccgcgt
     1801 cgcccgcctg ttcgaccggt tgcggcgcgc gcggtcccaa gaggatctcc tccgtctcca
     1861 cggcgcggtc gtcgccccct tttcggcgct cggcgccacc ttcggcggcg ctctcgatcc
     1921 cgcgttgcgg gcgatgctcg acctgctgtt cgcccagaag ggctcgctca tgctggtcaa
     1981 ccggtggggc aatctcatcg tcgaggcggc gaccaaccgg gccatcgcgg gcatcgaggt
     2041 gccctacgac cacgcctcgc cggcgtggag cgtcatggag agcggggaac cgctcttctg
     2101 cgaggatatc ctgaactacc cccggttcaa gaagaaactc gacgcgtacg ccaaggacta
     2161 tttcctcatc gtgcccatca aactgtacgg aaagaccgtc ggcgtgctca acctgagcga
     2221 caagacggtc gcgctcctgt tcgaccgggc cgacctgctg cgggcgcagg ggcttctgcg
     2281 tcttctcgag ccggtgttgg cggcgcgttg cggtcttttg gcggtgcggt gaggtcggca
     2341 tgataggcgt cggggtcatc taccatctcg cgttgctggt ggcgctcagc accgcctccg
     2401 gtttcgtgag tcaacgctgg ctgtggcgac ggtccggcgc ggtcctgcag ggccttatct
     2461 tcggctgcgc ggcgggcgtc gggatgctgg ccccgctggc gctcggcgac gggctggtct
     2521 tcgacggccg ctccatcgtg ctcagtctct gcgggctctt cttcggtccg gtcgccggta
     2581 cgagcggggg cggcatggcg gtggcggcgc gactcctgcg ggggggcgac gataccctgc
     2641 tcaccgagat actgatcata ctcctctcga cggtcctcgg cgcccttttt tatctgcgac
     2701 ggtccgaccg cgaggcgccg atagggaccg accggctcct gttcttcggc ctgctcgtcc
     2761 atgtcgcgtc cctgcttctg ctctacacgc tccccttcga catcgctctg ctcgccatca
     2821 aacgggtcgg cgcggtcttc ctgttcgccg cgccgctggt gaccgcgctg gtcggcagga
     2881 tactttcgga cgatgtgtcg cggctccggc ttgtcgagga cctgcgaaag cacaaggagc
     2941 gtttccgcac ctttttcgac gccaccgccg acatcgtgac gctcaaggac gccggggggc
     3001 ggtatctcat ggccaacagg gcgcagtgcg cgttcctcgg gaagaaggaa ggcgaccttc
     3061 tcgggagacg cgacctcgat cttctgccgg aagagacggc tctccgttac ctccggtccg
     3121 atcaggaggt cctgcggacg aaaaaggata tggcctacca tgaaaaggtc ggggaccgcg
     3181 tctacgaggt ccggaaattc ccggtcccgc tccgggaggg ggggctcggg gtcggcgcat
     3241 tcgtccgcga catcaccgac cgcgaacggg cggaggggga actgcgcgaa agcacggcgc
     3301 gctccgcggc gatcctgcgg tcggtgcccg acctgatctt cctgataagc cgcgacgggg
     3361 tgttcctcga ctatcacgcg aacgacccga tgctcctgtt cgcgccgccc caaagactgc
     3421 tgggccgcca catctccgcg gtgttctcgc ccgagttcgc cgaaaaactc gagaccgcgc
     3481 tcgcgatgac gctcctgacc gacgagatac ggtcgatcga atacgaggag gaaagcggcg
     3541 agacggtccg ctgtcacgag atgcgtctgg tgagaagcgg cgaggaggcg gtcttcatga
     3601 tcgtccgcga catcagcgac cggcggcggg ccgaaacgga tattcaggag atgaaggaac
     3661 tgctcgaaga gcgggtgcgg gaacggaccg cgcaactcga ggaggcgaac cggcggctgc
     3721 gcgaggcgcg gaccgaggcg gaacgggcga gcaaggccaa aagcatcttt ctcgccagca
     3781 tgagccacga gatacggacc ccgatgaacg cgatactcgg ctatgtgcag ttgctccgtc
     3841 gttccgagcc gctcaccggc atccagcgcg agcatctttc gaccgtcctg cgcagcggcg
     3901 aacacctgct cgacctcatc aaccagatac tcgaactgtc gaagatagag gcgggccgca
     3961 tcacgctcaa ccccgtcgat ctggacctgc cgcgactgct cgccgacctc cgcgacatgt
     4021 tcctcccgca ggcggtcgag aaggggctgc gcttcgaggt gcggcaggac ggcgccatcc
     4081 cgcgttacat caacgccgac gcgataaagg tgcgtcaggt gctcatcaat ctcgtcggca
     4141 acgcgatgaa gttcaccaaa cggggcggga taacgctctt agtgcgggac gacggcccca
     4201 ccttctacgg caacgatcac ctgataacgg tggaggtcgc cgataccggc ccgggcatga
     4261 cgcccgaaga actgtcgcgg atgttcgacc cgttcgaaca gaccggcagc ggggcgcgga
     4321 gcggcggcac cgggctcggg ctcaccctca gcaaacagtt cgcctccctg atggaaggga
     4381 cgctctcggc gcggagcgaa ccggggcgcg gcaccgtcgt gtcgttctcc ttccgggccc
     4441 tgccggcgac cgcggcggcc gcgaccgcgc ggacccggtc gccgatgaag cggctgccgc
     4501 ccgggctcct ctacaaggtg ctcatcgtgg acgacaacgc gtccaaccgc gatatcctgc
     4561 ggatactgct caccgagggc ggcttcatca cccacgaggc gaaggacggg cgcgaggcca
     4621 tcgcggtgtt ctggaaatgg cggcccgatc tggtcttcat ggacctgcag atgcccgaga
     4681 tggacgggag ggaggcgata cgccggatac gggaaagccc cgaggggaag acgataccca
     4741 tcatcgtcat aacggcggac ccgctcgaag agacccggcg cgagatgctg gccgccgggg
     4801 cgaccggttt catccgcaag ccgttccgcg aagaggagtt gtgcgacgcc gtcatgaagg
     4861 cgctcgatat cccgtgcgac cccgcgccgg tcgcggaaga gccccgtccc cccgcggtcc
     4921 cgccgccggg cgtgagcgag gcgccgctcc gggtccccga cgcgatacgg cgcgaacttc
     4981 acgcggcggt cgtcaaaggc gataccgggg agatgctgcg gcttctcggc tcgctggggg
     5041 agggggatcg gccggtcgcc gaccggctcc gcgcgctggt ggacgatttt tcctacgaga
     5101 ccctgctcga actgacggag gatgcccatg actgaacggc acggtgttcc cgatatcatg
     5161 atcgtggacg atacgccgca gaacctgcgg ttgctctcgg aactgctcgg ggcgaaaggc
     5221 taccgggtcc ggccggtgcc gaacggcgcg ctcgccctgc gcgccgccgg ggccgagcca
     5281 cccgatctca tcctgctcga catcaacatg cccggcatga gcgggttcga ggtctgccgg
     5341 agactgaagg ggacggcggg cctgcgcgat atcccggtgc tcttcatcag cgcgctgaac
     5401 gatacgcagg acaaactgct cgccttcgag gcgggcgggc aggactacat caccaagccg
     5461 ttccagttcg aagaggtgct ggcccgggtg aacacccacc tgaaactgga gcgcctgcaa
     5521 cgggaactgg cgtggcacaa ccgcgaactg gaggaccggg tcgccgaaca ggtgcgcgag
     5581 atatccgagt cgcaggtggc gacgatcgtg gcgctcgcga aactcgccga atcgcgcgac
     5641 gacgacaccg gcaaacacat cgagcgggtc cagatattct gccgcctgct cgcggagaaa
     5701 ctggccgaaa gccccgcgtt ccgggaccgg atcgacccgg cgttcgtcga gaacatcttt
     5761 tacgccgccc ccctgcacga tgtcggcaag gtgggcatca gcgacagcat actcctcaag
     5821 ccggggaagt tgacccccga cgagttcgag atcatgaaga cccacagcat gatcggctgg
     5881 cggaccctgc aggcggtctg cgagagatat ccccggaacg cctttctctc catggggcgc
     5941 gtggtggcgc ggaatcacca cgagcggtgg gacggcaacg gatatcccga cagactcaag
     6001 ggcgacgcca tcccgctcga ggcgcgcatc atggcggtcg ccgatgtgta cgaggcgctc
     6061 cgggcgcgcc gctgttacaa ggaaccgttc gaccacgaaa aaagccgggg gatactcgcg
     6121 ggcaacggcg gcacgcagtt cgacccttcg gtgatagcgg ccttcctcgc gctcgatacc
     6181 gagttcgacc ggatcaggac cgatatgcgg gattaggggc ggggcgcggc gttatttctc
     6241 ctcgtcgtcg taatagatgg tctctttctc tttcacatag gcgtcgatgt agacgcgatc
     6301 ctcctcggcg atatcgacga aacgcaggcc catcccgggg gtgacgccca tcgagtcgga
     6361 cgcctccggt ccgcggaccc agcggacctc cgcctgtgcg ttgatgttcc gcccctccac
     6421 cgcgaaggcg atcttcatct tggtcccgat ggggagtgtc tgcagggtgg aaaggaagat
     6481 gccgcccttg ctgatgtcct gcgtgatgcc ggtgaagaat ttgtgcggcc cctccgccgc
     6541 caactcgaac gacaccgtga attcggcgcc gatgcgggtg tcgcgccgct tgtcgaatat
     6601 cacatcgtca cggtcgcgtt tggtgatgtc gtcggtcatg ggttccctcc gtgtctagga
     6661 cccgcgaagt atagtgcggg aaaggggaaa aatcaagcgg gaagtgggag aaatgctgtt
     6721 acggattttc atggcgaact ctgttggatt tcgtagaatt gatgcaaaaa aaggggactt
     6781 tttaatcatc tacgacaagt atggtgcgat cccatgagct gtgatgacca gacggtctct
     6841 gaccagaaaa ctttttatcc gttatctgga tattgctcat tctattgaga tcaaatcttc
     6901 tccattctgg gagcttgccg gaactgctgc ttccatcaat ttggtatcca agtaattgga
     6961 tgattccggt gtgaataccc aagacatgtg gttcaactac tcggtgatga cttgagtagt
     7021 agaactcaat gattgctttg gttcgaattg cttcttcaat cacactttgg ttcatgagaa
     7081 cttctcctta tgtttctatt tgtggatatc taacattagc catgaggcac ggcttccatg
     7141 gttttggttg aatgtcgatt agtcatttaa ggatgccttt aactcgtcaa gattcttcca
     7201 tgaaacagca ccatgaggta gtggtttatc gaagtccagc aaggcatcaa gcaattccga
     7261 cacggtcatc aagatcgctg aatccgcctt gagcgagctt aagacattgt acggtttgtc
     7321 ggtcttggcc tcctctccaa ggtatgcgcc ctcgaagttc ggaacggagg cgaccatcct
     7381 cgttggccct gggcacttcg ccatctcgat ccggatcgag tcattcagcg cccaggccgg
     7441 atttgccgac tctttctcat tcttcacata cgttggacga tcactgtcgt gcagaacaga
     7501 atagggtgcc ccgaactggt tcagtatttt gcagagcgag acaattgtcg ccttgccgcg
     7561 tgcgcggaca acatggacac cctcgtattt cttcggggct gcagcaatcg catgcttgaa
     7621 tgctgtgtac tccgtatctc cttcaacgat gatggttcgc cctccgaaga aaaactccgc
     7681 aacgtatgga tcacagagat tcagcaattt caggcactcc ttgtccgttc cgtcgaacat
     7741 tgctctttca gggcggaaga tggtagttcc tgtgatttgg ccgtcctctc ttctttcgac
     7801 acggacaata gaggtgtgat ctcgggcaaa gtctacgaag caaggggagt gtgtcgtgac
     7861 catgacctgc cagttaccag tgatgcccgg caagccgtag agcagatcgc atgcttccct
     7921 aaccgcgctt gggtgcaggc atatctctgg ctcgtcaagg aggagtacgt gtgggcgttg
     7981 tacgccctct ggtttcttct ttccggtctc ggccaatagg cgtatggctg tccagagtag
     8041 ggtacgtcgt gccccgctac cctgtcggcc aaccgagctc aagaagccat cctcgggtcc
     8101 catgagtagt tgaggattcg ctttgaagag gttgatggct ttgtctaagt catcttcggg
     8161 tttggcgtcg aatacaacgc gatggccggg gaaaacgcga ccaatgtagc cggacagatc
     8221 cgcctgaacg gcggcaatat gctcctcgct ttctgctacg atcttcttct gaagagctgc
     8281 aatgcccccg agaagtttag cgaagtcgga ttccttgtct tccttggagt tttcttgatg
     8341 ggactttacg cggtccttga ttgccatcat gagcagcttg acgatttcac ctgcctgctc
     8401 gtccggagag gcgaaggcgt ccacgcgatg cggctgtgga cgccgtgagt tcgcaacacc
     8461 agcagcaccc catggtactt tctctctgtc ctcatcggtt gcccacctgt tctccttcac
     8521 attaaaacct ctgcgaaccg gtgtaccggg tgctggccaa cgccactgtt ccctgaccaa
     8581 cttctcccct gaagaagtcg ttgctatcca ctccgctcca ggactgttgt cgtatatcac
     8641 ggtgtggagt tcgatttcag ggactgcccc agaatcaacc tttccgaaag ggaagtcctc
     8701 tatcgcgaga tgtcctgcgt tcgagccttc agacatgatt acttcatagg ccttcaacac
     8761 ggaacttttg ccgacattgt tgggaccaac cagcacaaca atatcatcga gatcaagcgt
     8821 gactggagag gctccgatgc agcggaaatt tttgatgatg agcttggaca agcgtggccg
     8881 tggttccaat gttacttcgg gtgcggtctc cacagctttc actttcttcg ccatgatgtt
     8941 ttccctaaat aaaattaaat ggttactata aagccatcct aaaagctatc ctattaaagc
     9001 tatcggtaaa gccatcgaag ccatcgggct attggggtta catctatatt ttaccataat
     9061 ttcgcttcct gttccccatc ctcgcctgcc gaaatcttcc gtcgggaacc ctgtcgaggc
     9121 cacatctaaa tgtatatttc cctgccccgc tccccatcct catccaccta agtccccata
     9181 catagcggag ccggggcggg acccgaaaat ccctttggga ggtatcgagc gtaagtttcg
     9241 ggtccgcccc atggaacggc tccattccgg ggggaaatgc acgatccgtc aggggatgcc
     9301 ccgatggatc cgtatctgct gaaattcgtc gtaactcgct ctccgcgccg cactagagtt
     9361 ttctccatct cacctttccc gccggctgaa gcgtctcatc caaaaggacc cgcgcctcat
     9421 acgcatcggc gtcctgttgc gccgcccgcg accggatacc ccgctcctcc tcgccggaaa
     9481 gcgttgtcgg aaggttcggt tcgacggagg ccttcacctg cacaaggatc ttcctgccgc
     9541 ccaccccggc cgcctcgatg tccgcggccc cgggcgccag agaatcccat ttgatgatgg
     9601 agaacctctt cgtcttcaat tcctcgatca ccgctttttc accggcccgt ccggtctcga
     9661 taggcatggt catatcctgc tccttcatgg tttcgtatcc gttatttcca ttggcatctt
     9721 caccacgaac gcgtcgctgt tgccggtgtt cgcgcacccc ttgaaggcat tgcgggtctc
     9781 gcccacaacg atgagcgacg atgttttctc gtcccacacg gcggcgcggc agatatcctc
     9841 acccgtcgtg ccgaactgta cggtcttcgg cgtttcgccg agcgtcgtga cgaaacagtc
     9901 ggttccgccg ggagaaacag cgtcttccat cacgccaagg gtgtacccga agaccgagac
     9961 gacatcctca tgaagggcca tcccttttaa ctcctcgacc aacaaggtgc ccacaaaccg
    10021 cgttgaaata aggattcctt ccggcgacaa cgcagaaacg aagatgtcct gacaacgctg
    10081 tgacggataa caagggctta tagtatattc ttccgaagat ggatctagaa aaacaccggc
    10141 tcccgtaccg gcaacgacaa cggttccgtc tgtttttatg atcaggtatt cgccgcttct
    10201 cgtttcattg gcgtccgcat cggtaacgcc gttctccttt tgccaaacct gtgtccatac
    10261 cttttcaccg agcggagttc ttttgcttgc gaatgcgccg ggaaacggtt cgccgaaata
    10321 cttcaggcca ccgataagcg tattcccttc cagttcgctc tgcgtatagc cggtgatgaa
    10381 gaggctccct tcggcgtcaa gcgtcattcc cgccgacaca tcatctttgt cgccgcccca
    10441 ctgtttcagc catgcgacct cgcccttcgg tgtccattcg gcgaggaaca ggtcggcgtt
    10501 cactttgtca tggcattcgg tctcgtcctt cggtaggccg tcgagcgacc ctcgggtatg
    10561 accgaacatc aggatgttgc ctgccccggt catggcaaga gcggtcgcgt agtcctcgtc
    10621 ctcggtgcgc atttgttcta tccatgccac cgtaccgtcg ggagatatct tcgcaaggaa
    10681 gacatcgggg tctgagtgac gccaccacga gtgcgcgaag tgaccgtagg tgtggcccgt
    10741 cacaaagatg ttgcccgtac cgtccactac cgccgctgcc gcgatgtcgt tgtccgctgt
    10801 cccgaactgc acggtccatg ccgccgagcc gtcttctttc cgcttggtca cgaagatatc
    10861 gccgagaccg gaggcagacc tgccagggaa cgctccttcg gtgttgccga ccatgaccgt
    10921 tgtcccgtcg tgagattccg ccaccgcgac cgggatgtct tccccgtcgg tgccgaattg
    10981 acggaccgtg taggattccc cagcgcatgc ctcgtcttta taggagggtg gttcttcgta
    11041 gaagtagtcc atccatttga aaacaagatc gttaaaggat tcgccggtat cgggagagac
    11101 ccacatttca ttggccgcca ccaacaattt cccattcgtt tcaaccatgt ccgcaaaaga
    11161 gaaataggcg ttcttttctt cagaaagcgg cccgtataga ttctttttca aacgccttcc
    11221 cgtgtcggca tcgaagacca tgagttctgg atgtcgggct tcttgttttc gtgtgcctcc
    11281 gtcacccact gcccaaagga tgccgctgtc gtccaagtgc aaccggatag atctgtcgaa
    11341 ataaaaagag gtggctccgc ggtactccca gagaagagca ccatccggat catacttgcg
    11401 aatggtcgaa tcgccccatt gtgcatgagc aaacaaactt aatgcgtaaa cactgttctc
    11461 gacatcggtc accaccgaaa caccccagtc aaagggatta gcggttgcgc ttccccattg
    11521 acgagcccac agcatattcc cttcaccatc caacttcata agatacggat cgggacagga
    11581 agatgtctcg gcgggaccga aaggcaaccc ggcacaatcg cccaccttgc cggcattatg
    11641 ctgatcacca agaccgctta ccgtatagcc agtaatataa atattccctt cgccgtcgag
    11701 agcgatatac ccccccatat cgcccccgtt gccgtatatc ttttcccaga tcagtttgcc
    11761 ttcaaaatcg gtcttcgcta catagatacg ggtataactg gcggctcctc cttccggcca
    11821 actcgccgaa aaaccggtca caaagatacc gttggcaccc acagcaagcg ttcccagttc
    11881 gtcctgagaa tttacgagtg cgacctgcca atcgtatttc catgaccgtt cccagagaag
    11941 cgtcccttct tcggcattga atgcggcaag gtaaaaagtg ttcgagttcc taccggacac
    12001 gatgaccttg gatggatcag ggccataggc aaggccgata ggcgccacat agatatcggg
    12061 ccaacaataa ctccaacgct ccgaaagatc gctgttatac cgtataagaa gcgccccatt
    12121 gagtccgaca aaggtgtcct catttttaac ggcgatgatg accccgccat cagcggtcgg
    12181 aagcatcccg gcaaggagat cccggtcggc cctatcgatg cgaaaatccc agaaaggttc
    12241 ggcatccggc acagcatcct catccgaagc gagaaaatcg tcggaaaagg caagagtatc
    12301 gtcgttcact acgatatcgt catgaggaag agaatcatga ggcgagcagg aaaaagcgag
    12361 aaaaaccaaa agagctatgg ctctcacaat tcgcccgttc tgtttcagca tatcgtgtct
    12421 ccttgtattc tccccctccc gtcatcaggc gggaggggga gaacgcattt atttaacctg
    12481 cctttcctct ttccggatca tcatcaatcg cagaagttat aaagattggt ttcacacaga
    12541 tcccacaccg gagagaattc tccaccgtca ccatctgaga ggatatgaac ccaaccgaga
    12601 tagagataaa tgggtatcca tattttcagt atagggttgt aggaccaagt aagagactga
    12661 ggattaacac tgaatctaac tgaaaaaaca taagggctat gatcacattg ataacatgca
    12721 ctagaaccgc tgtagtaatt aagcagttct tccccaaagg ttgtggtcgt gcacacatac
    12781 gctccttccc cggaagaaca ggaacaattg ctaagggtac cgtaccgttg ggcggttatg
    12841 gtgcatacat tgcttcctgt gatattactc catacggggg caaggggatt tgatgtggtg
    12901 tttcgagccc acatgaactt gtaactgaca agatccccta taggggaaac actatagacg
    12961 ccgaccatca tcagtctata atactgggga taccagatgt ctccaaacac ccaatcctgt
    13021 tgtatggttg ttgagtcgag cgcggcatac cctccagaga gggcttcctc cgccgcctct
    13081 ttcgttgcct caaccatgaa cggcgtgaaa accaccgctc cttcgggcaa ctctttcacc
    13141 gcttcgtcat aggcgatctc gtactcgtag ccgttttctt ccagatactt caccgcttcg
    13201 tcaagcgata tctcctgcgc gaaaagaatg ccggaaaagg caaagaccat aaaggcgaga
    13261 aaaactatcc gtttcatgat gctcactcct tttgttgtcg ttttgttttt actttttttc
    13321 tcatcttact ccttccgttc ctctttttca agagagcgga ggtcccctcg tgttgataaa
    13381 caaaaaaacc catcgcgcca tcccgctacc atccccttat tttttcccag attttgtttt
    13441 ctcatttcgc cgtacttata tctgttccgc gacccattcg taatagtcgc ggtcgccctt
    13501 ggactcgttg tgttcgcggc acatggggta gaggttccag accgaggtct ttccgcgttc
    13561 gccccacggg acccggtggt cgagggtgtc ggcgtggttc gtacagcccg gcacggtgca
    13621 caggtcgccc ttcagcgcgc ggaccgtatc ctccatctcc tgcggccact cgcgctgatc
    13681 gacataggcc tccaactgct cgcgcggcgt cggcgggaca tgccgttttt tgcaatgggg
    13741 acaggggatc tcctccctcc gcgttctcca ctcgacggag atgccttttc gacagtccgg
    13801 acagcggtaa aaatagttca tggggtctct cccgttctca gatgatcctg cgggtatggt
    13861 cgtgtcgaag gaccggcggg ggaaagaagg cgggacgatg cctcccgggg tcgtctggag
    13921 gtatcggagc cctccgggtc gacatcgccc cctacggcta cgctatcggt cgcatccgct
    13981 ttgcgaagaa aggaccattt ctcctgcgcc agcgcccaca ggcacggcaa caggcgttcg
    14041 cgtacgatga aatgccgtat gagccgccac cgcgaacttt tcccttccac caccccgacg
    14101 atatcggcga gggatatcca cgggtcgcag agcgggttgt tgtccccttt ggtcagcgcg
    14161 cggccgcgcc atgtcagcac caaccggtgg gcgacgaaga tgtcgtcgta ccggaagacc
    14221 accacatcgc cggaacgcag gcgcgtccgg tcgccggccg gcaggatgcg gataagttca
    14281 ccggggagta tggcgggtat catgctgtgc gcgaagccct tgatgcttat cggcttcggg
    14341 tcgtcgccgc tgaagaacag gtcgaacaac cgatccagat gcatcacccg tcccgactcg
    14401 ttgcatgggg gattgttacc ggtcaccaag cacctctctt caccttttcc tacgaacacc
    14461 cataagatac ggcgagcggc ttaaaggatt caggtgctcc gaaatattta ggtaactatt
    14521 tggaataaaa gggggataat atcgttgcgg gtagccgagc ggtcagctga tctttcacca
    14581 cccacttttc ataccagata tcgcggttcg attttccgcc gggtataatg gtcaaagcat
    14641 caccgactat tgaaatggag cgatcatcat gaacgataac tttggcgatc ctttcatttt
    14701 gttttcggcc gaaatagcgc cgtgaaagtt ccgtataagt gtcgtccatc tgtatcatca
    14761 cgaggcaggg gaaatccttt tccttatcat cgacgacaca atgctcgacc gcgccggcgg
    14821 caagaaaaac tttttggttc atttgcgttg ccgcggagat tttgatcccc gttgtttccg
    14881 gtaggaggcg gcttgtcgtc gtatcatcgg caggatcaaa ctctgttatc accaccgagc
    14941 cttttgagag agcatcgaat gcgcgtcgcg ttccgataag gatgatccgc ccattcaatt
    15001 cagtcgccgc accgacgatt tcatcttcgg cggtcagtac ctgccgacaa gaaagcagat
    15061 tcccgtcgcc atcataccgg gcaaagaaaa tatcgcggtc aacggtttct ttcagtgagc
    15121 cggcacacat gtctcctctc gttgttcccg cgacaactat tccgccatcc gacagcaaga
    15181 atatagcgtc tgccgattcg ttttgaggcg tcctaagttg caccacccaa cgatttcccc
    15241 atgttgcggt atcgatttta cgcacgaaca catcttggcc gtaagattcc tcggtgagtt
    15301 gtccgggatc accatatata tcgccgataa cccaccccgc gatgtaaatc tccgaaaaaa
    15361 ctccgtgcgt catacttgtt atgcgggtcg gtggtcgaag tacattcacg attggatccg
    15421 ggatcaatcc ttttcccatc tcatacagca tggtgttgac aactttgtct ttgtaatcat
    15481 atccaaccag caccgcttcc tgacaaaaaa gaccggtgca gttggcaatg gccgagatcg
    15541 caccacccag tatcgccata tttccgtcaa caagcatgct cgcgatttcg tcatccgcat
    15601 atgtttctcc gttccacccg aattgagcga taggttctat tatatcggat gagacagtgg
    15661 ccaagaacat gtcgcggcat tccctgccct cgcatttggg cgataatttc atggtgtcct
    15721 tcatagcata ctcggcaaaa ctcccttctg taaatcccga gagcaaaagc gaaccattgg
    15781 tggttgatat tgccacatcg aacacaaatt cattgtacgg acttccggct gtaagacttt
    15841 tgctacgcga ataatgatcg agtgtttccg gcacgatcag cgagtcatta tcgggaccat
    15901 cttgatctcc cgaaaaatca ccatcaaatg gactgacatc cgtatcgcct tcactctgtt
    15961 ttccatcgct gcaactgaga caacacacga gaaggagaag aaaactcaca tatgcaccta
    16021 tcttattcat ggagctccct ccgcagatac agatcggaac aaccggctct gtcgcaggtg
    16081 gttttacggt cctcatattg ttcttcgaac gaagcataag tggtcccagc caagtaccgg
    16141 taaccgccat ggatgagcac tgcattgatc gtcgtttggg gtgtcggtgt catttcaatg
    16201 ttttccacct gacccgtaga acgatctata tccacccaaa agccataggg cactccggta
    16261 tattctccgg taacagccat atcaccattg gtatctccaa cggcaacgag gcttccgtca
    16321 tcgagaatga aaagatgcga aatgccttca cttcccttgg atgctacctg tgtgctccac
    16381 aacatattaa gttttttgtc gtattttacc acataggcgt ctgtgtctgc tcctgtgccc
    16441 ctgccgcccg atgggggaaa atgaccatta aattcatcgt aataactcat ggatagggca
    16501 acatatagat cgtcgttctc atcggaaatg atagccgtgg cgtattcaag ggcttccccc
    16561 atcatatacc ccgactggct gacgatcagc ttgtcgacca ctgccgcgtc cttgtctagg
    16621 atgagaacaa aagcgtcttc gccgttatcg ttaatatcgg taatgcccgc cgccgctatc
    16681 atgccgttgg tcagacttgt tgccgaattt gcgcttagcg gtacgcggag ggtcgtttct
    16741 tcataaacga cattttttgt gatctctccc gttaaagaca ggcttgctat gccaatacca
    16801 tcatggttat cactcgagaa aaccattgcc ccggaattat aaataagaag cgcgccgtca
    16861 tcagtgataa cgatctctac gggatcaaac atcttcgcaa aagagagagc aagtgggcgc
    16921 gtaaactgac ggacaaacaa gatcgcgcct ttctgatcta agcgggcaac gaacatgtct
    16981 tcgtaaggat ggatcacact gtctgatttc gcatgtccga aaaactctcc ttgggtccat
    17041 cccgtgagcc agatagagtt atccttgcct atcgccaacc ttttaagaaa gtcagaatat
    17101 tccgttccaa aagactcttt ccatgcgtag cgtccgtcgc cgtcaatttt tacaagataa
    17161 atatcgtaat cgccgttgtt ggggacattc cacggttcgc catacgaggt gaatccaagc
    17221 accatgttgt cctcgccgtc ccggagtagc cgcgcggtca tatctcgata gaccgtcgat
    17281 atctgtgtta cgcccgattc acaacagacg gcatttacac aactgagaaa aacatcacag
    17341 gtgccatcga tacgacaggg ttctccaatg gctccctccg gtaccgaaac agcgtcgtca
    17401 caattgccgg aatcacagac caacccgaag tcgcacgaac catcactatg acagggggag
    17461 gcgctcaaga cgccccccca aaccaaaaac agaaataacc ggcgcaacat gttggttagt
    17521 ccgaggtaat gatcaacacc ttctccgcaa ggatgttcct gtctgacacg tactttaccg
    17581 cagggatgat cgtaccagtc tcatccatgt agtaaatacc tgtgtactca tagttttcca
    17641 tattatccat tttttcttgg cttgcaatct ccgaaagaat agtttcatgg tctttcgttc
    17701 taacattctc ctctgaaaca aagatttctt ttggagcatt cgaaaacacc tttgacagac
    17761 cagaacgggt aaaatacatt tttatttttt tgttatcaac aaacttcccg tttatcgcgg
    17821 gcttataaat gagatgcagg ccaatagcaa cctcatttaa gggctcgcca tctttgatgc
    17881 ctgtcaaggt ttcgaaggat gcgctttcca gttgcatttt atagtcatac cacttctcca
    17941 aaaggccagc cgattctttc aaagcaaatt cataaataat tccggtctcg tcttccggaa
    18001 tgacagattc aacctctttt ttcccccatc caaaatgacc gttgtttttg ttgaaataat
    18061 aatattcgtt ttcgtcgaga ttgatcttct gtgaggaatc atagttttta atatagaaca
    18121 tgtttagaaa atcattttcc gtgaatgact ttgacatata aatggtttcg tctac
//
LOCUS       DAOQUE010000002        17925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_3596_length_17925_cov_4.593005, whole genome shotgun sequence.
ACCESSION   DAOQUE010000002 DAOQUE010000000
VERSION     DAOQUE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17925
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3596_length_17925_cov_4.593005"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="PKH10_00090"
     CDS             complement(<1..1469)
                     /locus_tag="PKH10_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT26550.1"
                     /translation="MKPSLTIFLSIVLAVVVTIVGCASSPEKKKEMKEDQGVEALQDD
                     GKKAAGASPTPQKPEEKKQAKTQKADEKKKEDVDKAPDSKEEADNLRNKNYDGLKYTG
                     PAQKRFVSGVERYFTEGCEGTIPVWKEALSSDGDMGTIAYNIGLCYDRQDKVDEARRW
                     YLQAIQARNRSVEALYNMALLDMRKGVLKADEYERLAGTFEDAVERSNFLAWLYLKTG
                     KHEESIKQAKAALKEDEMNVPAMVTLGTVYFHKEMFELAEMILSTAEQIAPNDFKLQR
                     IYGFLEYKQGAGHKQKATEHFQKAKKINPEMPEISNMLAILAMEIEDFATARKELEFA
                     LKIAPQFVEAKINLALALKGLKEYAKARDILLEIEKSPGLSKALSQDVRYNLAILYLD
                     ADVDGAGTPERFDASAEYFSKYLELVDKKDKAERKRIDDYKKEALTEKKKLESMIKMR
                     QKLEAKKKKKEEEDRLWQEFLKDAEEAKGKDTIEAYEAFL"
     gene            complement(1466..4873)
                     /locus_tag="PKH10_00095"
     CDS             complement(1466..4873)
                     /locus_tag="PKH10_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT26551.1"
                     /translation="MMRISPRSFFILLATLFPAIGFGEETTAEQSPEEPLPIAVDGAS
                     FEENYVERQILRYEDRILDYQREIQSLLRRKVEEKRRLIEMKYKPSMDQESQKEKESR
                     ADAIVLFENFIKKYRGNAEYAPSAMYRLAELYYERSALEYEEEYKKYEEQIALYEKGQ
                     LAQEPVEPVVDLGPSIALYREIINSYPQFRYLGETYYMLGYYLDASKKSDEGISVWEE
                     LIRKDLFTKYNAEVHLRIGIYYFTLEGNDEKETKKNLYKALDHFKKSAEFKDYKARDK
                     AVYNTAWTYYRLAVGPDAEKYFPLAIDTFASLVFYADEMKKRGVNEGQDMRKESVQYL
                     AVSFSDEFWGSVEKAIDYFSKIDGRSFEVDVFDKMGKFYYENVDYEKAEQAYRHILKK
                     YPYYADAPKIHKKLIDMYAMQERLAEYQDEIELFVKTYDADSEWSIKNRANATILRDA
                     GNQAKEFLIASAGYHHKAAREAKKAENGELSQKEYLIAAQLYGKYLERFPYTSESYDV
                     SYFLAESLYFSGQVEKAVLVYERVRDDKNQDSNRDDAGLMVYRCYSDIWEDSAERQKK
                     GEEKRGTPLSKLEEKLVESGDVYLKISKNADDRSTVAFLLARIFFEHGNFEEAEKRYL
                     SIINEFPDSELALAAARDIIRAYDEKKDWVNLAKWSKIFGERFSSSTKEKDRGKKEEE
                     EFRTSRGFALFRYAEQLETEKKHREAAEEYLRIVEENPYYKDADAALFNAAQNFASAF
                     MFDSALTIHERIYKEYPYSPFAPKSLYRVAINAERAYDFEKARDAYLLLYKKYPTDER
                     KNDAIFNAAFLLEKLKRYPEAATYYALYAREEAGKPEGREAAFFVGFMYQKAEDWKRM
                     VTEYNRFIDTYRSDPEVQNLVMRAYYQIAQTYEEKMNDWKNAKAVYAKMLEHYNAQKP
                     EGAEANTYAAEAEFKLLEDEFAEYEKLKIGGKDEKKLAESLQAKMAASQTLSKKYDNV
                     LRYRSEWSYAAFYRQGNILQTFAEALINSPPPPEIEKDPDLVDMYREGLQQKIDPLEQ
                     QAVDLYVRTLDLARQNQVANKWITLIIERLARLRPQEYRAEKAPRYGMNTAKETGYPI
                     ALTLDRAERRAYKKAGMHDNKEDEPTKGSSQEKVAPPASDQPKEQKE"
     gene            complement(4870..7059)
                     /locus_tag="PKH10_00100"
     CDS             complement(4870..7059)
                     /locus_tag="PKH10_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT26552.1"
                     /translation="MEKELSILEDDFKGMEGRYLSPFVASRDYTESPSKKMQKADFYF
                     QQKDYISSGGIYYSVFSVRKERDFIWEESLFKLAESLFLNKNYISASRYYEILLAEKP
                     YSRFQVEALKRLIASSYYLGEYSKAKTYYAEFLNIGYDMARDQELVYFLAKALFFDKQ
                     YDEAQEIFKTVKNESTFYPQSLYFLGVITLQRGEMDTALSFFKQLTDLPGEKGYSRYE
                     AVRDLAILAQGRILFEKGDFPGATDAYLKLDRGSDLFAEAYFELCWAYIRREQYEKAL
                     DALRLIKYIAPGSLVVPQAALLEGNLLIKLRKYAEAMLLFNNVVKEYADIRNELNTLK
                     KSGRSFFDQSLGADEGKFSLYSPLVRSLLKNNKKFSHAIQLHDDLKSIEVELEQVSKM
                     ESKLASIVESKNAASIFPPLKEGTEQAISSQNRLSFLRNEILKVRAEIALSFLSEQDR
                     KRYQALEQEKETMQKKIADLMPKSTKDIEEKVARNAKQLLAFGEELHRSSIRMKALYD
                     QLDAVGEYYLRQAGQPENADPRITEKIEQEKKRIQEMMAQITFFKKDTEDDKNRMLLG
                     GNMLAQMVFVRDAYDKIIAEQEKLIILAPVAAGGEHQKAKEISARIDDLSKKITLFNE
                     KLNEVIGGIIQNIKDSFESEKIKIEEYKSQLLSLRRETAEIASLAMHTNIAKVNQVFD
                     DLVLKADQGIIDVAWEKKDEATQNLKRLIIRRAQEMRELNLNLENLE"
     gene            complement(7181..7921)
                     /locus_tag="PKH10_00105"
     CDS             complement(7181..7921)
                     /locus_tag="PKH10_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HNT26553.1"
                     /translation="MRNLWRRGVFSLVLGFLFVLWSQEISQTTPLGTVSDEDGAEVPL
                     SNAEQGEKTEQTTDGEQEVNLKLRNLEEKINSLKDKVFRAKQRLATLQETVLSGALAG
                     ARASVVHLNEVGSTFDLVSVVYYLDEAPVFKWVEGSGVDIKTGMSVFDGSVVPGPHHL
                     SVYLIFRGSGYGVFSYMRGYTFKLKAGYSFNVEEGQLVEVSVTAEDRGTLVKLDNRLY
                     ISFDIKKKVFEQRPQTDAVEGTIKPAAE"
     gene            complement(7981..8787)
                     /gene="murI"
                     /locus_tag="PKH10_00110"
     CDS             complement(7981..8787)
                     /gene="murI"
                     /locus_tag="PKH10_00110"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNT26554.1"
                     /translation="MGYALSGDLPVGVFDSGIGGLTVLKEIRRLLPQEHLLYLGDTAR
                     VPYGTKSPEVVRRYSELNIRFLLEKKVKTVVVACNTASSLALDYLEELFPGIPIMGVI
                     APVISNLALLPGLRRIGVIGTTATIRSNRYQQLIRGQQQVEVLSQACPLFVPFVEEGL
                     VDHSLTRQVIDHYLRDLKRADIDTLILACTHYPLLSAMIDEYFDHRVRIIDSAYYTAL
                     ELKKMLENAQLLNPSSEEGGESFFVTDTIETFRDTATRFFGRPLSNIQHI"
     gene            complement(8842..9453)
                     /gene="cglE"
                     /locus_tag="PKH10_00115"
     CDS             complement(8842..9453)
                     /gene="cglE"
                     /locus_tag="PKH10_00115"
                     /inference="COORDINATES: protein motif:HMM:NF033755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adventurous gliding motility protein CglE"
                     /protein_id="HNT26555.1"
                     /translation="MKKLLTILLIVAFSALWAEEEEAGDASGNDLDPVLKGLQATMSG
                     GIFAIIGKAADYSDPIGSVVNLSFGYDLQIVDAFSLGMSAKFGILQLNTVTNKSASPW
                     YQAGDYAPIVVGADISPTYCINERWEIGANISIDYNMMAEAEDSSGKKQSGFLVVGGG
                     VDAEYYTYARHFSLGMMAEFNYIVNFDGFGVTVMPYLKYSFGI"
     gene            complement(9477..10895)
                     /locus_tag="PKH10_00120"
     CDS             complement(9477..10895)
                     /locus_tag="PKH10_00120"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNT26556.1"
                     /translation="MPNEKVQKRLLYDLSPIPRPPINEGFVGKLDPEVRVVYKPNILR
                     ESKVLTKGYLAGSSTDTSKVDLMLDEAIFPEDSFELVSLVGDQMTVNLKDGFKGYVVE
                     KKERKDLSSVVATKKSYEIGPDNFLVLEFGDLSVVALRSPHEKLKPASLIENREVAPF
                     LILIVTFILGLIIMNLVMETPPIEVDIFEIDNRFANMIIGNQDDVEVEDIEKEKKVKL
                     EKPKEGLVEATQQRKSLGEEGRVGDKKSRVANAQGSARRGLDEKVAQSSGVMGALSAG
                     AASFDRIFGGGGLGAGMDKTLGSVTGISGVDQFGSGGLGTRGFGTGGGGNALGIGGLG
                     TRGRGGGGMGGMGYGMAAGRIGGKGLSDITAGGGQAVIMGALDRAVIDAYIKRNLAKI
                     RWCYEKELNKDPKLFGKIVINFIISKTGQVSSSKVQRSTMGNENVESCVADQIKKIRF
                     PAPKGGGIVIVNYPFVFKHSEG"
     gene            complement(10940..11221)
                     /locus_tag="PKH10_00125"
     CDS             complement(10940..11221)
                     /locus_tag="PKH10_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26557.1"
                     /translation="MKKFFALCFLVLFVAGAFGQEEAVDTGDRDTDGRKIIYRSKTVI
                     DFEDVMLEGQIKKPSGSFLMDRTKTKFNSLINLKKDFNKELVKSVDVLK"
     gene            complement(11249..12418)
                     /locus_tag="PKH10_00130"
     CDS             complement(11249..12418)
                     /locus_tag="PKH10_00130"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT26558.1"
                     /translation="MRKIFSLIGLAILTGAFALSCATAKKDEDATVKKEPEKKRLSVT
                     DRPTTPEEWTKFHTQKGIDALEVKKGKADFAGAIKHFTAATEAYPKSPTVWFNLGLAY
                     QQMGDDENAVKNYNAALQLEPTYARPVINKAAIYANKLEYNRAIDVCEKFLEENPKDK
                     AVLSAVAGFYLKKLDFMKAIESVRKILIQEPSNVDALKNLGMIYLIKKDTGLANMVTT
                     NSYNLNPNDAGITNNRGVIFHYQADDSLAMFYYSNALNLDQGNRAANHNVGSMAMSYG
                     DAERAFNHFSVVLAQDPDNLDARIGLALAHRGMGNFTEAEAEYKKVLDKDPNNPLAVF
                     NLGVLYHDHLNKKVEAQSLFRKYLSIGGEIGQDHIVHKYLADKVKIRPSEDEQTN"
     gene            complement(12432..15929)
                     /locus_tag="PKH10_00135"
     CDS             complement(12432..15929)
                     /locus_tag="PKH10_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT26559.1"
                     /translation="MKKTLVALLVILVAGSLFAAREKRKTDEKDMKGPAKMDTESAKA
                     KIQIDSKAMAQRDKAIEQLLELLKDFPEGPKKAEVYRRLAELYWEKARGIKAIIMEEY
                     NKKTDKYYEINDPNAPMPELNLKEAWKWNEKAVDTCDFIIKKYPNFEGIDEVYFFMAS
                     NLMEVGKPLPAIRYYTLVTEKYRNSKYAADAFYEMAEYFFNNNNVFKAMPAYKAIIDS
                     YPNNPFFGFALYKYAWCLYNVGEYEESIKVFQQVVEYSNKSNKVELKEEALKDMVTPY
                     AEAGSVDDAEKYFKSVVKEKKYFVMVLIKLAGIYFEQDKNEEAIKIYRKLISEVPNAP
                     ECPMWQKQIVEAYKKLNMKDNVRLEILVLVEKYGNKESSWSKANIKDEATVESATQTA
                     EANLRILLVEYHNEARKTKAEATWRIVEELYPKYLKYYPKSEASYDIRFNYAEFLWEN
                     KRYETAGNEYQIVADLNPKGHHFEDASYGAVSAFGMLLKEKQEAQNRKIKDSIASKKK
                     AGDTGIEESRVVIAGQGKMTGKKALPAEYQPKEIPPLEMKFIKACETYIKNIPRSKYL
                     VDIIYKQAITYYSFNHYQESVPVFEMIVQKYPRDDLAVFSADLIMDSLNITEDWEAIS
                     NKAREMLRNSALISGRNRLKDDLEKFKEMATFYAAELPAQNGKTIDSAERYIAFVTEF
                     PSSRFNDVALNNAIYYYAKGEDLYKSIRVQERFLGENAKLYKESKLRNAIMFGLAKNY
                     EAIAYYDKAAQLYIDFVAKAGQDKNVGDAIFNAAVLYEALGDTEKALDNYKLYIDKHL
                     KDDKEKAKIALQFGYIWMRKGKTYYDKARKGFERFLGDHISMKGLKDFRYIDKSGKLT
                     GKADTSAVEVSKGSPNTLFALYAAQVKMAKEEGRTEDYYKFHDAILQLYKNGQFKDKE
                     DFAEGTRDTIADALLVEADARFREFMAIDLNVKYDKTIKYKPEEINYADESKIPKNIK
                     KEMDTFNKETADRLTKKAEMLVELEQRYMDIIAQTKSPRLTPACLYHIGAMYRNMTDK
                     MFTAPLPPWLNENQKIVYKNMLDEKALNARQKAVDALEKAMLKGYETSVYNDWVKKAK
                     YDLRYFQELRPGKYYDENELIPRPNMIETSSLIGRVDMDMKFPPISDEEKKDILRKMT
                     GAAVAPVPAQQQPVTGSTN"
     gene            complement(15970..17598)
                     /locus_tag="PKH10_00140"
     CDS             complement(15970..17598)
                     /locus_tag="PKH10_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26560.1"
                     /translation="MKKIALALFFILLSGLFVSCAEQLTVREQEMKKYENIDPSVAQG
                     MPDAIRKVVDAYNDGDYHTAAIGFYAIMKNSEWARLHETARYYYAESLFRIGLYQAAE
                     YQLAEILFEGPGGHYFVSALLKLLAVTYETKDEKVLFAVLSNVNYNALPKKFANELMY
                     YLGKINFYRGQDEEALKFFTQVKDYSSFYPLATYFMGVIQVRQRMYAEAMQSFQKIME
                     MPDDMYVGADIKALKGMAELALGELYYAAGFDEKTKNKLAMFNVALNYFNNVDRRNPQ
                     WFESLFARTWASLMISRFDSTLGTVVTLGSPFFTDIYFPDVYVVEAVTYYNLCRYQEV
                     NEVIDKFFRVYPDYRQLLQSWLENVSTKTGLDVYKELLVMYRDASAGKKTPIPEAILR
                     SILSDQKFQKEYIHVKEIEREAAIIENSPESFKKSVIADDLMKKMNYQLATIRNEAGK
                     MQVKKIQEITQDLTQIIADMKAIRFEMTDKLKATYEKEELYGKKLKESALKEEQSYSP
                     AVPDRHYYWPFDGEYWQDELGYYFYNIQNACMDE"
     gene            complement(17614..>17925)
                     /locus_tag="PKH10_00145"
     CDS             complement(17614..>17925)
                     /locus_tag="PKH10_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26561.1"
                     /translation="KLSLFGDFIPKYDINVSLGGNYYQVLLEKGTKTKTGHNFAVNAG
                     FGARFFITNNFFLSLNLDWFLMQDERFKLGAVAAEATSKYLKTDSLFSINFGMFLPFA
                     D"
BASE COUNT         3862 a         4820 c         4328 g         4915 t
ORIGIN      
        1 aggaaggcct cgtaggcctc gatcgtatcc ttacccttcg cctcttcggc atctttgagg
       61 aactcctgcc acagtcggtc ctcctcctct tttttcttct ttttcgcttc cagtttctgc
      121 cgcatcttta tcatgctttc taatttcttt ttctcggtca gagcttcttt tttgtaatca
      181 tctatgcgct tccgctcagc cttgtccttt ttatcgacca gttcgagata ttttgagaag
      241 tactccgctg aagcatcgaa tcgttccggc gttccagccc catccacatc ggcatcgaga
      301 tacagtatgg caagattata gcggacatcc tgcgacaacg cctttgaaag gccggggctt
      361 ttttcgatct cgagcaggat gtcgcgcgcc tttgcatatt ctttaagccc tttcagcgct
      421 agggccagat tgatcttcgc ctcgacgaac tgcggagcta tcttgagcgc gaactccaat
      481 tcctttctcg ccgtggcaaa gtcctctatc tccatggcaa ggatggcgag catgttcgat
      541 atctcaggca tctcgggatt tatctttttt gctttctgga aatgctccgt cgccttctgc
      601 ttgtgcccag ccccctgttt atactcaaga aaaccgtata tccgctgcaa tttgaagtcg
      661 ttcggggcta tctgttcggc ggtcgacagg atcatctcgg cgagttcgaa catctccttg
      721 tggaaataga ccgtcccgag cgtgaccatg gcggggacat tcatctcgtc ttccttgagt
      781 gccgccttcg cctgcttaat gctttcctcg tgctttcccg ttttgagata caaccatgca
      841 agaaaattgc ttcgctcaac cgcatcttcg aaagtcccgg ccagacgctc gtactcatcg
      901 gccttcaata cgccctttct catgtcgagc agcgccatgt tatacagcgc ctctacgcta
      961 cggttccgcg cctgtatcgc ctgaagatac caacgccgcg cctcatcgac cttgtcctga
     1021 cgatcgtaac aaagcccgat attgtaggcg atggtgccca tgtccccgtc ggaggaaagc
     1081 gcctctttcc agacaggtat cgtcccttcg cacccttcgg tgaaatatcg ttctaccccg
     1141 ctgacgaaac gcttttgcgc gggtccggta tacttgagcc cgtcatagtt cttgttgcga
     1201 aggttgtccg cctcttcttt gctgtcaggc gccttatcga catcctcttt tttcttttcg
     1261 tcggctttct gcgtctttgc ctgctttttt tcttctggct tctgcggggt cggagaggcg
     1321 ccagcggcct tcttcccatc atcctgcaaa gcctcgaccc cttgatcctc cttcatctct
     1381 tttttctttt ccggcgaaga ggcacagccc actatggtaa cgaccaccgc aagaacgatt
     1441 gacaaaaaaa tggtcagcga gggcttcatt ccttttgctc cttgggctga tcggaagcag
     1501 gcggcgcaac cttttcttga gacgatcctt tcgtcggttc gtcctctttg ttgtcatgca
     1561 tccccgcttt tttataggcg cgccgttccg cgcggtcaag ggtcagcgct atcggatagc
     1621 ctgtttcctt agcggtattc ataccatacc gcggcgcctt ctccgcgcga tattcttgcg
     1681 ggcgaagccg ggccagccgc tcgatgatga gcgttatcca tttattggcg acctgattct
     1741 gcctcgccag gtcgagagtt cgcacataga gatcgaccgc ctgctgttcg aggggatcta
     1801 tcttttgctg taacccttca cgatacatat cgaccagatc ggggtctttt tctatttcgg
     1861 gcggcggcgg cgaattgatg agggcctcgg caaaggtctg gagaatattc ccctgacgat
     1921 aaaaagcggc ataggaccac tccgaccggt atcgtagcac attgtcgtat ttcttggaca
     1981 aggtctgcga cgctgccatc ttcgcctgca gagattctgc gagtttcttt tcatccttgc
     2041 cgccgatctt gagtttttcg tattcggcga actcatcctc aaggagtttg aactccgcct
     2101 cggcggcata ggtgttggct tcggctcctt caggtttctg ggcgttataa tgctccagca
     2161 tcttggcata aacggctttg gcgttcttcc aatcgttcat tttctcttcg taggtctgcg
     2221 ctatctggta gtaggcgcgc atgacaaggt tctgcacctc cggatcgctc cgataggtgt
     2281 ctatgaaccg gttgtattcg gtcaccatcc ttttccagtc ttccgccttc tgatacatga
     2341 aacctacgaa aaaggccgct tctctcccct cgggtttgcc cgcctcttcg cgggcataga
     2401 gcgcataata ggtggccgct tccggatagc gtttaagttt ttcaagcagg aacgccgcgt
     2461 tgaaaatggc gtcgtttttt ctttcgtcgg tcggatattt cttgtacagc aaaagataag
     2521 cgtcccgcgc cttctcaaaa tcgtaggcgc gctccgcatt gatggcgacg cgatacaatg
     2581 acttgggagc gaacggggaa tacgggtact ccttgtatat gcgctcatgg atggtcagcg
     2641 cggaatcgaa catgaacgcg ctggcgaaat tctgtgcggc attgaaaagg gcggcgtcgg
     2701 catctttgta atacggattc tcctcgacga tacggagata ttcttcagcc gcctcacgat
     2761 gtttcttttc tgtttcaagt tgctccgcgt atctgaaaag tgcgaatccc cgtgaggtcc
     2821 tgaactcttc ctcttccttt ttgccgcggt ccttctcttt ggtcgaagaa gagaatctct
     2881 ctccgaagat cttcgaccac ttcgcgagat tcacccagtc ttttttctcg tcatacgccc
     2941 gtatgatgtc gcgggccgcc gcaagggcca gttcggaatc agggaattcg ttgatgatgc
     3001 tcagatagcg tttctccgcc tcctcaaagt tgccgtgctc gaagaatatc cgcgccaaca
     3061 ggaaagcaac ggtgcttcga tcatcggcgt tctttgatat cttcagatag acatcgccgg
     3121 actcaaccag tttttcttcc agtttcgata acggcgtccc tcttttttcc tctcctttct
     3181 tttggcgttc ggccgaatcc tcccagatgt cgctgtaaca tcgatacacc atcagacccg
     3241 cgtcatcgcg gttactgtcc tggttcttgt cgtcacggac ccgctcatat acgaggaccg
     3301 ctttctccac ctgaccggaa aaatagagag attcagcgag gaaataactc acatcgtaac
     3361 tttccgaggt ataagggaac cgttccagat acttgccgta caactgcgcc gcgatgagat
     3421 attccttctg ggacaattcg ccattctccg cttttttcgc ctctctcgcc gccttgtggt
     3481 gatacccggc cgacgcgatg aggaactcct tggcctgatt ccccgcatcg cgaagtatcg
     3541 tcgcattcgc gcgattcttg atggaccatt ccgagtcggc atcatatgtc ttaacgaaca
     3601 gttctatctc gtcctgatac tcggccagcc gctcttgcat ggcatacatg tcaatgagct
     3661 tcttgtgtat cttcggggca tcggcatagt agggatattt cttgagaatg tgtcgatagg
     3721 cctgttccgc cttttcgtaa tccacatttt cgtagtagaa tttgcccatt ttgtcgaaca
     3781 catccacttc gaaagagcgc ccgtctatct tgctgaagta atctatcgcc ttttcaacac
     3841 ttccccagaa ctcatcggaa aaactgacgg caagatattg gaccgattcc ttgcgcatat
     3901 cttgcccttc attcacgccc cgttttttca tctcgtcggc atagaagacc agagaggcaa
     3961 aggtatctat cgccaaaggg aaatattttt cggcatcggg accgaccgcc agacggtagt
     4021 aggtccatgc ggtgttgtat acggccttgt cccgcgcctt gtaatccttg aactcagcgc
     4081 tcttcttgaa atgatcaagc gctttataca ggttcttctt ggtctccttc tcgtcgttcc
     4141 cttccaaggt aaaataataa atgcctatac gaagatgaac ctcggcatta tacttggtga
     4201 aaaggtcttt ccgtatcaac tcctcccaca cggagatacc ctcatcgctt ttcttcgacg
     4261 cgtccagata gtaacccaac atataatagg tctcccccag gtatctgaac tgggggtagc
     4321 tgttgattat ctcgcggtag agagcgatcg atggtcccag atcgaccacc ggctcgaccg
     4381 gttcctgggc aagctgccct ttctcataga gagcgatctg ttcctcgtat ttcttgtatt
     4441 cttcctcgta ttccaaggcc gatcgctcgt aatacagttc ggccaaacgg tacatcgcgc
     4501 tcggcgcata ttcggcatta cctcgatatt tcttgatgaa gttctcgaac aggactatgg
     4561 catccgcccg ggactctttt tccttttgag attcctgatc catcgatggc ttgtatttca
     4621 tttctatcag gcgccttttt tcttccactt tgcgccgaag aagtgattgt atctctcgct
     4681 ggtaatcaag gatacgatcc tcataacgaa ggatctggcg ctcgacataa ttctcttcaa
     4741 aactcgcacc atccaccgcg atgggcaatg gctcctccgg ggattgttcc gctgtcgtct
     4801 cttcgccaaa acctatggca gggaagagcg tcgcaaggag aatgaagaac gagcgagggg
     4861 agatccgcat cattcaagat tctccaggtt cagattgagt tccctcattt cctgggctcg
     4921 gcggatgatc agtcgcttga ggttctgcgt cgcctcgtcc tttttttccc atgcgacatc
     4981 gataatgcct tggtcggctt taagcaccag atcgtcaaac acctgattga cctttgcgat
     5041 gttggtatgc atggcaagcg aggctatctc ggccgtttcc cttctcaagg aaaggagttg
     5101 acttttgtac tcttctatct ttatcttctc actttcaaat gaatccttga tgttctggat
     5161 gatgcccccg atgacctcgt tcagtttctc attgaacagg gttatttttt tagaaaggtc
     5221 atcgatcctc gccgatatct ctttcgcttt ttgatgttcg ccgccggcag caacgggggc
     5281 aagaatgatc aatttctcct gttccgcaat gatcttgtca tacgcgtctc tgacaaagac
     5341 catctgcgca agcatgttgc cgcctaggag catgcgattc ttgtcgtctt cggtatcttt
     5401 cttgaaaaat gttatctgcg ccatcatctc ctggatgcgt tttttctcct gctctatttt
     5461 ttccgtgata cgcggatccg cgttctccgg ctgccccgcc tgtctcaagt agtattcccc
     5521 gacggcgtcc aattgatcat agagcgcctt catcctgatg gaggatcgat ggagttcctc
     5581 gccgaaggca agcagttgct tggcattgcg agcaaccttc tcttctatat ccttggtcga
     5641 cttcggcata agatcggcta ttttcttctg catcgtctct ttttcttgct ccagcgcctg
     5701 atagcgtttt cggtcttgtt cggaaagaaa ggagagggct atctcggcgc ggaccttaag
     5761 tatctcgttg cgcaggaaag acaggcgatt ctgcgaagag atggcctgct ctgtgccctc
     5821 cttgagaggc gggaagatcg aggcggcatt cttgctctcg acgatagagg caagtttgct
     5881 ttccattttt gagacctgct ccagttccac ttctatgctt ttcaggtcat cgtgcaactg
     5941 gatagcgtgg gaaaatttct tgttgttctt aagcaacgat cttacgagcg gggaatagag
     6001 cgagaatttg ccttcatcgg cacccagtga ttgatcaaag aaggagcgac cacttttttt
     6061 gagcgtgttg agttcgttgc ggatatcggc gtattctttg accacattgt tgaaaagaag
     6121 catcgcctcg gcatatttgc gcagtttgat aagcaggttg ccctccagta aggcggcctg
     6181 cggcacaacc aaggaccccg gcgctatata cttgatgagg cgcaaggcat ccagcgcttt
     6241 ttcatactgc tcgcgacgga tgtaggccca acacagttcg aaatacgctt cggcaaaaag
     6301 gtcggatcca cgatcaagct taagataggc atcggtcgcc cccgggaaat cacctttctc
     6361 gaaaagtatc ctcccctggg caaggatcgc aagatcccgc accgcctcat atcgtgaata
     6421 ccccttctcg ccgggcaggt ccgtcaactg tttgaaaaaa gaaagcgcag tatccatctc
     6481 tccgcgctgg agggttatga ccccgaggaa ataaagactc tgggggtaaa aagtgctttc
     6541 gttttttacc gttttgaata tctcctgggc ctcgtcgtat tgcttgtcaa aaaaaagcgc
     6601 ctttgcgagg aaatagacca actcttggtc gcgggccatg tcgtagccga tgttgaggaa
     6661 ctcagcataa tatgtcttgg ccttcgaata ttcgcccaga tagtaggacg atgctatgag
     6721 ccgctttaag gcctcgacct gaaagcggga ataaggtttt tcggccagga ggatctcgta
     6781 ataacgggag gcggagatgt agttcttgtt gaggaaaagc gactcggcca acttgaaaag
     6841 ggattcctcc catatgaaat ctctttcttt gcgaacgctg aacaccgaat aatagatacc
     6901 gccggaagag atatagtcct tctgctggaa atagaaatcg gccttctgca ttttcttcga
     6961 tggcgattcg gtgtagtcac gactcgcaac aaagggggaa agatagcgtc cttccatccc
     7021 cttgaaatca tcttcgagaa tgctcagctc tttttccatg gaggaaaggc gggcgccaac
     7081 ggaaggaggc gtccgctgtt gagcagaaac cgcgccgcaa cagcaaacaa ccgcgataat
     7141 gatatgtacg gtccatcgtg tcatgcgcat gaccggacga ttattcagca gcgggtttta
     7201 ttgttccctc gacggcatcg gtctgtggtc tttgttcgaa cacctttttt ttgatgtcga
     7261 acgatatgta gagacgattg tcgagtttaa cgagcgtccc tctgtcctct gccgtcacgg
     7321 aaacttccac caactgcccc tcctcgacat taaaagaata ccctgccttc agcttaaaag
     7381 tgtacccgcg catataggaa aagaccccat agccgcttcc acgaaatatc agatagaccg
     7441 ataaatgatg cgggccgggt accaccgacc cgtcaaaaac cgacattccc gtctttatat
     7501 cgacgccact tccctcgacc catttgaaaa caggcgcctc gtcaaggtaa tagaccaccg
     7561 agaccagatc aaaggtcgac cccacctcgt tgagatgtac cacggatgcc cgagctccgg
     7621 ccaatgcccc gctcaagacc gtttcttgaa gcgtggcaag ccgttgtttt gctctaaaga
     7681 ccttgtcctt cagggaattt atcttttctt ccaggttcct cagtttaaga ttgacctctt
     7741 gttctccgtc tgtcgtctgt tccgtctttt ccccttgctc cgcattgctc aagggcacct
     7801 cagcaccgtc ctcatccgaa acggtcccca gaggagtcgt ctgggatatc tcctgggacc
     7861 ataaaacgaa caggaatcct aaaacaagag aaaaaacacc ccgtctccat agattacgca
     7921 taaatcaccc tcgaaaaatc cgcttcacta tacagcgggg aataaccgaa atcaattttt
     7981 ttagatatgc tgtatgttgc ttaacggacg accgaagaat cttgttgccg tatccctgaa
     8041 tgtctctatc gtatctgtga caaaaaaaga ttccccccct tcctccgaag agggattcaa
     8101 cagctgagcg ttctccagca tcttctttag ttccagggct gtgtagtagg ccgaatcgat
     8161 gatccgtacg cgatggtcga aatattcatc gatcattgcg gagaggaggg ggtagtgtgt
     8221 gcaagcaagg atcagggtat ctatatcggc tctttttaaa tcacgcaaat aatggtcgat
     8281 cacctgacgc gtcagcgaat ggtcgaccag tccttcttca acgaacggga caaaaagagg
     8341 gcacgcctgc gacaggacct cgacctgttg ttgcccgcgg atgagttgtt gatacctatt
     8401 actacgaatg gtcgcggttg tgcctatgac cccgatgcga cgcaacccgg gaagaagagc
     8461 aaggttactg atcaccggtg cgatgacccc catgatcggg atgccgggaa aaagttcttc
     8521 cagataatca agcgccaggg aagaggccgt attgcaggcg accaccaccg tttttacttt
     8581 tttctcaaga aggaatctga tattcagttc cgaataacga cgcacgacct ccggagattt
     8641 tgtcccatag gggacccgcg ccgtatcacc gagatacaaa agatgttcct gagggagaag
     8701 tcgtcgtatc tccttaagga cggtcaagcc gccgataccg gaatcaaaca ccccgacggg
     8761 aagatctcca gacaaagcat agcccatgcg attagacctt tctggttatg gttatatcaa
     8821 gggagagatc tgtgaaagaa ctcaaatgcc aaaagagtac ttgagatacg gcatgaccgt
     8881 cacgccaaag ccgtcgaagt tcactatgta gttgaattcc gccatcatac ccaatgagaa
     8941 atgtctcgcg taggtataat attcggcatc cacgccaccg ccaacaacaa ggaacccact
     9001 ctgcttttta ccggaggaat cttccgcttc ggccatcata ttgtagtcaa tgctgatatt
     9061 ggcgccgatc tcccaacgct cgttgatgca gtaggtcggg ctgatgtcgg caccgaccac
     9121 gatgggggca taatctcccg cctgatacca tggcgacgcc gacttgttgg tgaccgtgtt
     9181 caactgtaat atgccgaact tggcactcat tcccaaggaa aaagcgtcga cgatctgcag
     9241 atcgtatcca aaagaaaggt taaccaccga tccgatggga tctgaataat cggctgcttt
     9301 gcctattatt gcgaatatgc caccggacat cgtggcctga agacctttca agaccggatc
     9361 aaggtcattg cctgaagcat cgccggcctc ttcttcttcg gcccagagcg ctgagaaggc
     9421 gacaatgagc aggatcgtca acagtttttt catacctaat attcctccgt acctttttac
     9481 ccttcggagt gcttgaaaac gaacgggtag ttgactatga cgataccgcc acccttaggt
     9541 gccgggaacc ggatcttctt gatctggtcg gccacgcaac tttccacatt ctcgttgccc
     9601 atcgtggagc gctgtacttt tgaactcgaa acttggccgg tctttgaaat gatgaagtta
     9661 atgactatct ttccgaacag tttcgggtcc ttattcaact ctttttcata gcaccagcga
     9721 atcttggcga ggttacgctt gatgtaggca tcgatgaccg cacgatcaag agcacccata
     9781 atgaccgcct gaccaccacc ggcggtgatg tcagaaagcc ctttgcctcc gatacgaccg
     9841 gccgccatgc cataacccat gccacccatg ccgccgccgc ccctcccgcg ggtaccgaga
     9901 ccgccgatgc caagcgcatt accgccacca ccggtaccaa atcctctggt gcccaaacca
     9961 ccggagccga actgatcgac cccgctgatg cccgtgaccg agccaagggt cttgtccata
    10021 cccgctccaa gaccaccacc accgaatatc cggtcaaaac ttgcggctcc ggccgaaaga
    10081 gcacccatga caccagaact ctgcgcgacc ttctcgtcaa gtccgcgacg cgcggaaccc
    10141 tgtgcattcg ccacgcggct cttcttatcg cctacgcgtc cctcctcgcc caacgatttt
    10201 ctttgttggg tcgcctctac cagaccctct ttgggttttt caagtttgac ctttttctct
    10261 ttctcgatat cttcaacctc tacatcatcc tggttgccga tgatcatgtt ggcgaaacgg
    10321 ttatctatct cgaaaatgtc gacctctatg gggggcgttt ccataacgag gttcataata
    10381 atgagcccga gtatgaatgt gacgatgagg atgaggaacg gggccacttc tctgttttct
    10441 atcaagcttg ccggcttcag tttctcgtgt ggcgatctca aggcaacaac agaaaggtcg
    10501 ccgaactcaa gaacgaggaa gttgtcggga ccgatctcgt aactcttttt tgttgcgacg
    10561 accgacgaga gatccttcct ttccttcttc tctacgacat accccttgaa cccatccttg
    10621 aggtttacgg tcatctggtc tcccaccaaa ctgaccaact cgaaagaatc ttcgggaaat
    10681 atggcttcgt ccaacatgag atcgaccttg gaagtatcgg tcgaagatcc cgcaagatac
    10741 cctttggtaa gcaccttgct ctccctgagg atgttgggtt tgtagacgac cctgacctcc
    10801 gggtcaagtt tgccgacaaa accctcgttt atagggggac gaggaatggg ggaaagatcg
    10861 tacagcaatc ttttttggac tttctcgttc ggcatgattc agaccccgtt cgttatggtt
    10921 gttgttgttt ttctcattct tactttaaca cgtcgaccga tttgaccagt tctttattga
    10981 aatccttctt caggttgata agagaattga acttggtctt tgtacggtcc atgaggaacg
    11041 aaccggaagg cttcttgatc tggccctcca acatgacatc ctcgaagtcg atgaccgttt
    11101 ttgagcggta tattatcttc cttccatcag tatcgcgatc gccggtatcg acggcctcct
    11161 cctgaccgaa cgccccggct acgaaaagca ccaagaaaca aagcgcgaaa aacttcttca
    11221 tggaggttct cccttgctat ctctttcttt agttcgtttg ctcatcctct gacgggcgta
    11281 tcttgacctt atcggcaagg tatttatgaa caatgtgatc ctgtcctatc tcaccgccga
    11341 tgctgaggta tttcctgaag agactttgcg cttcgacctt cttgttcagg tggtcgtgat
    11401 aaaggacgcc gagattgaat accgcgagag gattattggg gtccttgtcg aggacttttt
    11461 tatactcggc ctccgcctcg gtgaaattgc ccatcccgcg atgggcaagg gcaagcccga
    11521 tgcgcgcatc cagattgtcg ggatcctggg ccagaacaac ggaaaaatgg ttaaatgccc
    11581 tctcggcgtc accgtaggac atcgccattg aaccgacatt gtggttggcg gccctgttcc
    11641 cctgatcaag gttcaacgcg ttactgtagt agaacatcgc cagagaatcg tcggcttgat
    11701 agtggaaaat aacgccgcga ttattagtga tgcccgcatc gttgggattc agattgtacg
    11761 agttggtcgt gaccatgtta gccagaccag tgtccttctt gatgaggtag atcataccca
    11821 agttcttgag ggcatcaaca tttgagggct cctgaatgag gatcttccgg accgattcta
    11881 ttgctttcat aaaatcgagt ttcttcagat agaagccggc gacggctgaa agcaccgcct
    11941 tgtctttggg gttctcttcg aggaatttct cgcagacatc tatcgcgcgg ttgtattcga
    12001 gtttgttcgc atagatcgca gccttattga tgacgggtct tgcataggtg ggttcgagct
    12061 gaagggcggc gttgtagttc ttgaccgcgt tctcatcgtc gcccatctgc tgataggcaa
    12121 gaccgagatt aaaccagacg gtcggagatt tcgggtacgc ttcggtcgcg gcggtgaaat
    12181 gctttatcgc accggcaaaa tcggcttttc cctttttgac ctcaagcgca tctatgcctt
    12241 tctgtgtatg gaacttcgtc cattcttccg gtgtcgtggg acgatcggtc accgaaagac
    12301 gcttcttctc gggttccttc ttcactgtgg catcttcgtc tttcttcgcc gtcgcacagg
    12361 acagggcaaa agcacccgta aggatcgcca gacctatcag ggagaagatc tttctcatgt
    12421 ttcagtcctc ctcaattcgt cgaaccggtg accggttgct gctgggcggg aactggcgca
    12481 acggccgccc ccgtcatctt ccgtaggata tctttcttct cttcgtcact gatggggggg
    12541 aacttcatgt ccatatcgac cctgccgatg agagaagatg tctctatcat gttgggtctc
    12601 ggtatcaatt cgttctcgtc ataatacttg ccgggccgca gttcctggaa ataccgaagg
    12661 tcatatttcg cctttttcac ccagtcgttg taaaccgatg tctcataacc cttcagcatc
    12721 gccttttcga gagcgtcgac cgccttctgg cgggcattga gcgccttttc gtcgagcatg
    12781 ttcttgtaga cgatcttctg gttctcgtta agccaagggg gaagcggggc agtgaacatc
    12841 ttatcggtca tatttcggta catggcaccg atgtggtaga ggcaggcggg ggtaaggcgc
    12901 ggagacttgg tctgcgctat gatgtccatg tagcgctgct ccagttccac gagcatctcg
    12961 gcctttttgg tcaggcgatc cgccgtctcc ttgttgaagg tatccatttc cttcttgatg
    13021 ttctttggta tcttcgactc gtcggcgtag ttgatctctt ccggtttgta cttgatggtc
    13081 ttgtcgtact tcacattaag atcgatcgcc atgaactccc tgaatctagc atcagcttcc
    13141 accagaaggg catcagcgat cgtgtcgcgc gttccttcgg cgaaatcctc tttgtctttg
    13201 aactggccgt tcttataaag ctggaggatc gcgtcatgga acttatagta gtcttctgtc
    13261 cgcccctctt ccttggccat tttcacctgc gcggcataga gagcaaaaag ggtgttggga
    13321 gagccctttg aaacctcaac cgcggaggtg tcggccttac cggtcaactt accgctctta
    13381 tcgatgtaac ggaaatcctt aagtcctttc atcgaaatat gatcgccgag gaaacgctcg
    13441 aaccctttgc gggccttgtc atagtaggtt ttacccttcc gcatccagat gtagccgaac
    13501 tgaagagcga tcttcgcctt ttccttgtca tccttgagat gcttgtctat gtacaacttg
    13561 tagttgtcca aagctttttc ggtgtcacca agcgcctcgt aaaggaccgc ggcattgaaa
    13621 atggcatcgc cgacattctt gtcctgtccc gccttggcaa caaaatcaat gtacaactgc
    13681 gccgccttgt cataataggc tatcgcctcg tagttcttgg cgagaccgaa cattatggca
    13741 ttgcgtagtt tcgattcctt gtaaagtttc gcattctcgc cgaggaagcg ctcctgcacg
    13801 cggatcgatt tgtacagatc ctcgcccttt gcgtagtagt agatcgcgtt gttaagcgca
    13861 acatcgttga atctggaact cgggaattcg gtcacgaagg cgatatatcg ttcggccgaa
    13921 tcgatcgttt tgccgttctg ggccggcagc tccgcggcat agaaggtcgc catctccttg
    13981 aatttctcca ggtcatcctt gaggcggtta cgccccgata tgagcgcaga gttgcgaagc
    14041 atctcgcgcg ccttgttgct tatcgcctcc cagtcctccg tgatattgag cgaatccatg
    14101 atgaggtccg cggaaaagac cgccagatca tccctcgggt atttctggac gatcatctca
    14161 aagaccggaa ccgactcttg gtagtggtta aaagagtagt aagtgatagc ttgcttgtag
    14221 atgatgtcaa cgaggtactt ggatcgaggg atgttcttta tataggtttc acaggctttg
    14281 atgaatttca tttccaacgg cgggatctct ttgggctgat attcggccgg aagtgctttc
    14341 ttccccgtca tcttgccctg accggcaatg acgacccggc tctcttcgat accggtatca
    14401 cccgccttct tcttcgaggc gatactgtcc ttgatcttgc gattctgcgc ttcctgtttt
    14461 tccttgagca acatgccgaa cgcagaaacg gcaccgtagg aagcatcctc gaaatgatgt
    14521 cctttggggt tcaggtcggc gacgatctgg tattcgttac cggccgtctc gtaccttttg
    14581 ttctcccaaa ggaattcggc gtaattgaat cgtatgtcgt aagaggcttc cgatttcggg
    14641 tagtacttaa ggtatttcgg atagagctct tccactatcc tccaggtcgc ttcggctttg
    14701 gtcttgcggg cttcattgtg atactcgaca agcaatatgc gcagattcgc ctccgcggtc
    14761 tgagtggcac tttcgaccgt cgcctcgtcc tttatgttcg cctttgacca cgacgattcc
    14821 ttgttgccgt acttctcgac gaggaccaat atctccaaac ggacattgtc cttcatgttg
    14881 agttttttgt aggcctccac tatctgcttc tgccacatgg ggcattcagg agcgttcggc
    14941 acttcgctga tgagtttccg atatatcttt atcgcttcct cgttcttgtc ctgttcaaaa
    15001 taaatgccgg ccaacttgat aagcaccatg acgaaatact tcttttcctt gaccacgctt
    15061 ttgaagtatt tttccgcgtc atcgacgctt cccgcctcgg catacggggt gaccatgtcc
    15121 ttgagggcct cttccttcag ttcgaccttg ttcgatttgt tggagtactc gaccacctgc
    15181 tggaaaacct ttattgactc ttcgtactca ccaacattgt aaaggcacca cgcgtatttg
    15241 tagagagcaa aaccgaagaa ggggttgttg ggataactgt cgatgatggc cttgtatgcc
    15301 ggcatcgcct tgaagacgtt gttgttgttg aaaaagtact cggccatctc ataaaaggca
    15361 tcggccgcat actttgaatt gcggtatttc tcggtgacca gcgtgtaata ccggatggcc
    15421 gggagcggct tgccgacctc catcaggttg gacgccatga agaaatagac ctcgtcgata
    15481 ccctcgaaat tgggatactt cttgattatg aaatcacagg tgtcgaccgc tttttcgttc
    15541 cacttccagg cctctttgag gttcagctcc ggcatcggcg cgttcggatc gttgatttcg
    15601 tagtatttgt ccgttttttt gttgtactcc tccatgatga tggccttgat cccgcgagct
    15661 ttctcccagt aaagttcggc caacctccga tagacctccg cctttttcgg gccctccgga
    15721 aaatccttca gaagttccaa caactgttct atcgccttgt ctcgttgagc catggccttg
    15781 gaatcgatct gtatcttcgc ctttgccgac tccgtgtcca tttttgccgg ccctttcatg
    15841 tccttttcgt cggtctttct cttttcccgt gcggcgaaaa gggacccggc cacaaggatg
    15901 accaacaagg cgacaagggt ctttttcatc atgctagagg tcgtcatatc atttccttcc
    15961 catcaaaagt tactcatcca tgcacgcgtt ctgaatattg tagaagtagt aacccagctc
    16021 gtcctgccag tactctccgt cgaacggcca ataatagtgg cgatcaggca ctgcgggaga
    16081 ataggattgc tcctccttca aggcgctctc tttcaatttc ttgccgtaaa gctcctcttt
    16141 ctcgtaggtg gccttcaact tatcggtcat ctcgaaacgt atcgccttca tgtcggcgat
    16201 tatctgcgta agatcctgcg ttatctcttg tatcttcttg acctgcatct tgccggcctc
    16261 gttgcgtatg gtggcaagct gataattcat ttttttcatg agatcgtcgg cgatcacgct
    16321 ctttttaaag ctctccggcg aattctctat tatggccgcc tcgcgctcga tctccttgac
    16381 atggatgtat tctttctgga acttctggtc gctgagaatg ctacgcagga tcgcctcggg
    16441 gatcggcgtc ttcttcccgg ctgaggcatc acgatacatg actaaaagtt ctttgtacac
    16501 atccaagcct gtcttggtgg aaacattttc cagccacgac tgaagaagtt ggcggtagtc
    16561 gggatatacg cggaagaact tgtcaatgac ctcgttgacc tcctgatacc ggcacaggtt
    16621 atagtaggtc accgcctcaa cgacatagac atcggggaaa tatatgtcgg tgaagaaagg
    16681 gctcccgagc gtcacgaccg tgccgagcgt agagtcgaaa cggctgatca tgagagaagc
    16741 ccaggtccga gcgaaaagcg actcgaacca ctgagggtta cgcctgtcga cattgttgaa
    16801 gtagttcagc gcgacattga acatcgcgag tttgttcttg gtcttttcgt cgaaaccggc
    16861 agcatagtag agctcgccaa gtgccagttc cgccatgccc ttgagcgctt tgatgtcggc
    16921 accgacatac atatcatcgg gcatctccat gatcttctgg aagctctgca tcgcttcggc
    16981 gtacatgcgc tgacgcacct gaataacgcc catgaagtag gtggcaaggg gatagaagga
    17041 ggaatagtcc ttgacctgcg tgaagaactt gagcgcctct tcgtcctgac cgcggtagaa
    17101 gtttatctta ccgaggtagt acatgagttc gttggcgaac tttttgggaa gcgcattgta
    17161 gttgacattc gaaagaacgg cgaagaggac cttctcgtcc ttggtttcgt aggtgacggc
    17221 cagaagtttc aacaacgcgg aaacgaaata gtgaccgccc gggccctcga aaaggatctc
    17281 ggctaattga tattcggccg cctgatagag gccgatgcgg aacagggact cggcgtaata
    17341 gtaacgggcc gtttcgtgga gacgcgccca ctcgctgttc ttcatgatcg cgtagaatcc
    17401 gatggcggcg gtgtgataat cgccatcgtt atacgcatcg accacctttc tgatggcatc
    17461 aggcataccc tgcgcgaccg acggatcgat attttcgtac ttcttcatct cctgttcgcg
    17521 gaccgttagc tgttctgcac aggaaacaaa cagcccgctg agaaggatga agaaaagcgc
    17581 caacgctatc tttttcatac tcggtcccct ctcttagtcg gcaaacggca ggaacatgcc
    17641 gaagttgatt gaaaaaagac tgtcggtctt aaggtatttg ctggtcgctt cggcagccac
    17701 ggcgccaagt ttgaaacgct catcctgcat gaggaaccaa tcgaggttaa gcgaaaggaa
    17761 aaagttattc gttatgaaga aacgggcgcc gaacccagcg ttgacggcga agttgtgacc
    17821 ggtcttggtc ttcgtaccct tctcaagaag cacctgatag tagttgccgc ccaaagacac
    17881 attgatgtcg tatttgggga tgaagtcgcc gaagaggctc aactt
//
LOCUS       DAOQUE010000003        16965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_3857_length_16965_cov_7.280367, whole genome shotgun sequence.
ACCESSION   DAOQUE010000003 DAOQUE010000000
VERSION     DAOQUE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16965
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3857_length_16965_cov_7.280367"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..457)
                     /locus_tag="PKH10_00150"
     CDS             complement(182..457)
                     /locus_tag="PKH10_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SinR family protein"
                     /protein_id="HNT26562.1"
                     /translation="MNMAYIISYDLKDPGKNYEKLLHIIKGYENWARLGGSAYIIITT
                     KTPVEVRDSLNKALDNNDKLYVGLVKAPAAWQGMPDEVSTWLRNNLH"
     gene            complement(454..1203)
                     /locus_tag="PKH10_00155"
     CDS             complement(454..1203)
                     /locus_tag="PKH10_00155"
                     /inference="COORDINATES: protein motif:HMM:NF037889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNT26563.1"
                     /translation="MVTKKIREKPSSEVKKAGKKVKPESKGDISVAVISSTCKKRKEE
                     SLSAFDIFQKTIKRAKNLIETHEEGQDCTEQHFDCFRAAIVLSISALDAYVRTLVVDK
                     ILACLEEKVLSAKLKEYIKGLLNQDALIEAGRKYEFRERVEKAIRADFETKAFQGEYK
                     INSYMELVGYKDVFEDVSRSADRNKTKMRADIERFTKRRHVIAHCGDFDLNQTLHKEN
                     TIKKEQAKECISMVTLFAEHLDKITSAGPKE"
     gene            complement(1360..2187)
                     /locus_tag="PKH10_00160"
     CDS             complement(1360..2187)
                     /locus_tag="PKH10_00160"
                     /inference="COORDINATES: protein motif:HMM:NF037406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26564.1"
                     /translation="MDIKSPPEHYWHCSVKIVGKKDYSVVNDLSFQELERTIVLPWKT
                     RCNFTVAGTLIRSTESVESIKITHTDYPRSYYAECHNEHMRASGIADLATNRSLLPLS
                     KGTDFTYDLLFSGAQETSPEPNVKMIEQLCRRLPQAARILATRSRKKKAAYKIADEYD
                     VQDFLHALLRAYLKYSVQEDPLPKVAGAKSSRADISIEDLGVLIEVKYVRGPEDQKRL
                     FEEYSQDLVLYAQWPHLKTLVYLIYNSADLREAEALERLSTTQEIGGKRFDVKVILA"
     gene            complement(2268..2966)
                     /locus_tag="PKH10_00165"
     CDS             complement(2268..2966)
                     /locus_tag="PKH10_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26565.1"
                     /translation="MIINREENSKVPDIGFFEFLMKPLPDRVRVPYQNLKSSFRPLEV
                     RNFLPFPMVGNSSKDEPRNKNIEIWPNEWHSCYIHPQQHHIFSFFINGFFIFRTSYFE
                     DLKKDPRDWGEPVLAFENIIIEITRSLMLAKQMYKDTVGVNEKIAFKWTLTKTKKRTL
                     IPPNHSGWSLGGIYTCFAENVFIVRTYKYGELSEYISLSKDVIDKIFVAFNWPTGKSL
                     IDDFQKDFLGHFKD"
     gene            complement(2956..4527)
                     /locus_tag="PKH10_00170"
     CDS             complement(2956..4527)
                     /locus_tag="PKH10_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26566.1"
                     /translation="MAKINEIAFNNYRAFYGEHAPIPINGKNLLVYGENGSGKSSFCK
                     ALHDLVWGKIDSPHAQHRYAKDWSVTFEINKNPDIPQKLTLSSPDSGDKLSQEYLKSA
                     QSISPFLDYKKILRLYLKDESEQGEINLLPILEKLLANYPVAEKGGKLLKELGAEKGQ
                     YLATVLNTDLKESLSAILATFKLDIVINGFTCNAVGDPLTLTAELFGEPVVDYHEFLN
                     EARLSALSISIYFAAIKKLSSLMPNRLDILVLDDILIGLDMGNRLAVFDILKNHFADT
                     QTFFFTYDRAWYEAFKDRVEDTEKWFFYEMYAQSVKENGHVFERPFIRPNDDFWMKAR
                     QSCDNFNYDQCGNLLRKEMEKQIDGYLGAKDGNLEQKLHLANIKNQSKVILDQVQRIL
                     EAIQKAEQGNPTDPRSALISMQRTMTEIKNNFGIDSFFYLKELKNRICNPSSHNDSLR
                     PFYKQELENSFDAIIDLMKTNNIEIEWSSDRPCRHIRPIEAKDSETQQQQEEDPFNAE
                     IKNIQIVEERIKNDY"
     gene            complement(4514..8041)
                     /locus_tag="PKH10_00175"
     CDS             complement(4514..8041)
                     /locus_tag="PKH10_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HNT26567.1"
                     /translation="MSRKILEKIVKDFSRDELAFFFRSKSPKFEQKMEPLRGYDGDKF
                     DKFTVIGEIELKNRFKLAVVAVEKNDGLTERTSRKAQFDLAKKIMQERFAFHAAAIFV
                     FHDKKGLFRFSLIHRQYKGTKAEFGPFRRFTYFVSPQESNRTFLNRIGEAPFDSLDEI
                     RDAFSVEKVNKEFYQEIEALFHRFVSDDQVMYPDLSDDRKETKEFAVRLLGRIIFCWF
                     LKKKRSSTGIALLPDALLASKIVDSYTEYYHDVLEPLFFEVLNAPTNDRDPRSLLQIG
                     QFADTIPFLNGGLFDPEPGDKYIAGMNGVPSQSSRNIIVVPNKWFSDLFALLERFNFT
                     LDENTPVDVELSIDPEMLGRIFENLLAEINPETGNTARNATGSFYTPRAIVEYMVNQS
                     LKRYLVQAASVTEETADCLLSYHSEINCVPDEKARLRIIEALDRLKVLDPACGSGAFP
                     MGMLQKMLLILDRIDPGAERWLDRMLTRVPSQFKAMVRCNLKKEGPEYVHKMGLIQEV
                     IYGTDNQTIAVELSRLRVFLSLIIEARVDDAAENRGIHPLPNLDFKFVCADTLIRLNN
                     LQTGATDMAPQVQDLKDLRELYFTAYGKDKEQYKEEFTQKQAELFKFVTAWVKDSSDT
                     KLIAEWSPFKSAPAPFFDPEWMFGVKDGFDIVIGNPPYIQLQSNHGALAEKYAGQNYL
                     SFARTGDIYALFYERGAELLKEGGHLCYITSNKWMRAKYGEKLRDFFSSRTEPVILMD
                     FGGYAVFDSATVDTNILLFMKTKTNKDKENRERKAKACKIEKDFNTMIDLADYVEKNS
                     FTLSGLSSESWIISSGDESIIRKRIETLGTPLREWNVQINYGIKTGFNEAFIIDGKTK
                     NRLIKEDPKSAEIIKPLLRGRDIKKYRIEFADQWLINAHNGYASVGAPLVGSQNQKQP
                     QGIVPTINQIPPVDINNYSAVKKHLDQYREEIFKRYDKGDTPYNLRNCAYVEDFEKEK
                     IIYPDIMRLPRDAINHDDYPYFYLDTQGYYAEATNFILTGKSLHIIAAVLASRLGVYS
                     FVKFYTGPQFDEKGFRYKKEYLQNMPIPKAEKERADVFKNLIYYIQHLKVVDQPLMAS
                     FFETIIDNMVYDLYFEDLTRARDIHISEIIEKLARPFKENMTDAQKLAHCEKLYHDLK
                     NNNVVMRAIINRKNIDFIKVIEGTADGKN"
     gene            complement(8047..11367)
                     /locus_tag="PKH10_00180"
     CDS             complement(8047..11367)
                     /locus_tag="PKH10_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNT26568.1"
                     /translation="MFITNSGANKLEKRLNELIGFSEELKFLVGFFYFSGIKELLPAL
                     KENSSVTLKILVGLDTDALASNMVVEVARNDRSVAQIVDRYFESIKKGLRQDDFDTKE
                     YYEQSVYYIELIKSKRLHIRRTFDPNHAKLYYFKTKGEYQGLAKDKFITGSSNLTRAG
                     LTGQHEFNIEIGDFGTAEAEKYFDELWARGVPITEQEPIRKRLLDLIEMNTLVAKLTP
                     FEAYLLILKTYLDTFIDIDKKMPCSELLKKKGYQEFSCQTDAVAQSLTTIQNYGGVII
                     ADVVGLGKSIIAGLVARLLNRRGLIICPPGLIGDDKKTVGWKKYYEDFELHGWEIRSS
                     GDLEKTVEFVAEHPDFEVVLVDEAHRFRNENTRAYELLKNICRGRIVILLTATPFNNS
                     PADIFSLLKLFIIPGKSRITLDDDLEARFRTYDYLYGRLSYIVKNHNSKDAQKRKKAE
                     LEYEKLFVGRKPPIDLKEVQQRTRLLSNSIKEVISPVVLRRNRLDIKNDPVYSREIGA
                     LSETVPPKELFFALTPEQSRFYDTVIGEYFGEEGFFEGAIYTPYLYEKENPEDEDKKT
                     LEGESQKNLKDFMRRLAVKRFESSFAAFRQTVQNFIKINELVLAFIKKTDGRYILDRH
                     NMEQISQLDGDEMEEALLGFAEEMAEKGKKLKGRIYQTNTFVRREEFFAHIDSDLALF
                     RKILKEIGDLNLVAEDPKVASLIAHLPEIRGKKLSDEPERKVVIFTEYVDTLNYLGEK
                     LTKEFPDTVLVMPKDLRESGVQEILENFDASSKKQADDHMILAATDKMSEGFNLARAG
                     AIVNYDIPWNPTRVIQRVGRINRIGQKIFKELHIYNFFPTEQGAEIVKSREIAAQKMF
                     MIHNALGEDSRIFDPDEEVKPSSLFVKINKNPEEGEEENFATFVRRTYFDLAEKHPET
                     TERIKQFSPRVKTAKQHEDGSLLVFTRKGRAFFVRGIRKEDTVEELPLSEVIPLIACE
                     PETPSKELGPDFWRHYDLIRGAETKHRGTTANSIITKASNNLSSILGSLSPELEPYRP
                     FITTLHRDIDEYKTLSEYTLRKIADMPMDKPEKVRKVLEELAKELGEKYLETVEKMVR
                     DFESEIIIAVENQKSPKE"
     gene            complement(11885..12583)
                     /locus_tag="PKH10_00185"
     CDS             complement(11885..12583)
                     /locus_tag="PKH10_00185"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNT26569.1"
                     /translation="MPTNSLKDQLFAIGEEIVACSETCPGFVGSCKKARSQGITPRGL
                     IFDKAINEAPNGILFIGSNPGPASPLEKKLYIEKGLTYEAVLDYCGSLDTKFYERTKA
                     VADGLDALLPPNRRFGRNCVWTDLVKCQDGTRHTDMDILRTFRTCRKYLDGEITLLTE
                     KKNYRIVALGNEARNFLGAMLKRPFIAIPHPSRNHSYYKNLSTILKKRKDDIRNFWEG
                     KAPSILELNKKSRT"
     gene            complement(12598..13284)
                     /locus_tag="PKH10_00190"
     CDS             complement(12598..13284)
                     /locus_tag="PKH10_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26570.1"
                     /translation="MKNCYYINSTGPDENKLKTALCLFLGKITTKKILLAVPQKDNLD
                     GAIKNVFGEAFVKEICRNNRCGANGHEFSLMTEKKDHYNVFEGSVIAIYATQKLLDKI
                     DSILNVTDVLVVPWVEDDISEWRPRWGATDIENGIKSQGTVLSAVVKTRLDNLHDTVN
                     VSTGLAHPSDRDYAINMFEEMKTKGTLYNPIEVKNYLIAEKRWDPEHADQVYEIAKGI
                     FEGKKFRKSR"
     gene            complement(13281..14852)
                     /locus_tag="PKH10_00195"
     CDS             complement(13281..14852)
                     /locus_tag="PKH10_00195"
                     /inference="COORDINATES: protein motif:HMM:NF037531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26571.1"
                     /translation="MKDLVVKGDWWFPGKEQEKVRGELRFSEEKGSRLFLAPSNLPLI
                     KKTDIILGLTDNNDKITLYDCYLQRFGLIGLREYEFESRNLFWGVHFEDIPHFSRIDF
                     QSETLSQWVNIDGFTHREDITLRPGESAIFYKKPEDVKLGKWDCCTCYLIFKVVQISS
                     GRSSHKQELKQEVSLRLDYEEEVVFIDIESQAHALMNMLAFAADSPVYSNEMTGYTLA
                     TGEMPIGIYSRQHIRGSLTKEKEVSHFLFEFTDVHEHAPQFIEKWIDLSLGDLSPFTN
                     NYIAEKYIPSGFVERKFLEYVRCLEVYHRKTSRQNSMKLKERLLEIVGKYRYNIELFS
                     DYAVEEFCERAKNGRHYLTHYEEKADQERMSLNEMVEKTNKMKILIDICLLAEMGFSK
                     EDIDRKFHHYISMPYRIMAADSYIEKHLTDESEKIATKKAATYLIKHCSNEPYVILAL
                     CNMHTKEEREELAASIKVLKSIGMLQKLVSRGNVRIVFSKEYEKYVDKYYREKKKEEE
                     EVRSTSKRKGKEEGS"
     gene            complement(14940..15254)
                     /locus_tag="PKH10_00200"
     CDS             complement(14940..15254)
                     /locus_tag="PKH10_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26572.1"
                     /translation="MDMKRSEKIIKVAAKIEAVLNECPTPTPNQSIPRRIHFLAGQLS
                     GCDHYAMEKAYEIAHWASIFYSARKHAKYPGGSSSLWTKMALDLLERIRTQAKSREAA
                     GD"
     gene            complement(15294..15494)
                     /locus_tag="PKH10_00205"
     CDS             complement(15294..15494)
                     /locus_tag="PKH10_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26573.1"
                     /translation="MSKSTRNTGNSWTPEEMVYLRMLAAQNTPTRVIGLKLGRTEAAV
                     RAKASDLGLSLKPTNQSPYGTK"
     gene            15905..16270
                     /locus_tag="PKH10_00210"
     CDS             15905..16270
                     /locus_tag="PKH10_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26574.1"
                     /translation="MGKRLVQSDHDLMVAAYAKDLMKLGYEVAVDLPGLITPAQIGEH
                     IPDITAKDPGTGKFHVFEVETSDSINDEHTASQWLTFAVAAAIGNSQFHVIVPKAAYL
                     SACLRVKALTLNNVDVIGF"
BASE COUNT         3966 a         4018 c         3893 g         5088 t
ORIGIN      
        1 ccgagcgagt acgcgcaacg atgaaacggg aacgcggggc ctacggcctc aacggaagcg
       61 gaagcggacc ctggcttgtc ccgccatagc gaagcgacgg cggaagggtg gggagtaggg
      121 ggcaaaaaca gcattctccc gaacaaacaa tgccgccgtc tcattgctat tcggtcatta
      181 tttaatgaag gttatttctc aaccaagttg atacttcatc cggcattcct tgccaagcag
      241 caggggcttt taccaagcca acatacaatt tatcattgtt gtctaatgcc ttattcaacg
      301 aatctcttac ttccactggt gttttcgttg tgattataat ataggcggaa ccccccaatc
      361 tagcccagtt ctcgtaccct tttataatgt gtaaaagttt ttcatagttc tttccggggt
      421 cttttaaatc ataagatatg atatatgcca tattcattct ttcggacccg ctgatgtaat
      481 tttatcgagg tgttccgcaa ataaagtgac catgctgata cattcttttg cctgctcttt
      541 ctttatggta ttttctttgt ggagagtctg atttaaatca aaatcgccgc aatgtgctat
      601 aacatgtctg cgcttggtga atctctctat atctgctctc atcttagttt tatttctgtc
      661 ggcacttcgg gaaacatcct caaacacatc tttgtatcca acaagttcca tatacgaatt
      721 aatcttgtac tcgccttgaa atgctttagt ctcgaagtcc gcgcgaatag ccttttcaac
      781 tctttctctg aattcgtatt tcctccctgc ctcaattagt gcatcttgat taagaagacc
      841 tttgatatac tcctttaatt ttgctgaaag aactttttct tcaagacaag cgaggatttt
      901 atcaacaact aaagtgcgca catatgcatc taaggcagaa atcgaaagaa caattgctgc
      961 gcgaaaacag tcaaaatgct gctctgtaca atcctgtccc tcctcatggg tttctattag
     1021 gttctttgcc cgtttaatcg ttttctgaaa aatatcaaaa gcggagagag attcttcctt
     1081 tcgttttttg caagtcgaag aaatcaccgc gacggagatg tctccttttg attcaggctt
     1141 gaccttcttg ccagcctttt taacctctga tgaaggcttt tctctgattt tttttgtgac
     1201 caatcttccc ctgcttacat cttcagtctt accaagaatt ttatcattcc cctcctttta
     1261 agtcaacttt ttgctgtttc ctttttgctg tttccttgct acacaagtat cttttaactg
     1321 ccatataaaa aagggggctg ttgaataaaa gttttatcgt caggcaagta tcaccttgac
     1381 atcaaatctt tttcctccaa tctcctgcgt ggtggaaagt ctttcaaggg cttctgcctc
     1441 tcgcagatcg gcagagttgt aaatcaaata taccagcgtt ttgaggtgtg gccattgcgc
     1501 atagagaact aggtcttggg aatactcttc aaagagacgt ttctgatcct cgggaccacg
     1561 cacatacttg acttcaataa gaacgcccag atcctcgata gaaatatcgg ctcgactaga
     1621 ttttgcgcct gcgacttttg gcagagggtc ttcttgaacg gaatacttga gataagcccg
     1681 aagtagtgcg tgaagaaagt cttggacatc atattcatcg gcaattttgt acgccgcctt
     1741 tttcttccga gagcgagttg ctaatatgcg cgccgcttgg ggcaagcgcc gacaaagttg
     1801 ttctatcatc tttacatttg gttccggaga tgtctcttgc gctcccgaga aaagaagatc
     1861 ataggtaaaa tctgttcctt tcgagagggg taacaagcta cggttggtcg ccaaatcggc
     1921 aatgccactg gcgcgcatgt gttcgttgtg acattcagcg tagtacgagc gaggataatc
     1981 cgtgtgtgta atcttgatgg attcaacaga ttcggtggat cgaatcaaag tcccagccac
     2041 cgtaaagtta catcttgtct tccaagggag aactatcgtt cgttccagtt cttggaaact
     2101 gagatcattg actaccgagt agtctttttt tccgacaatc ttcactgaac agtgccagta
     2161 atgttctggc ggggacttga tatccatgta atcctccttt catattaaag tattgttttc
     2221 ccccgatagg gttgggcttc gtgcagaatc cgaagaacaa gtcattgcta gtccttgaag
     2281 tgccctaaaa agtctttctg gaagtcatcg atgagagatt tcccggttgg ccagttgaat
     2341 gctacgaaga tcttgtctat aacatctttt gaaagagaga tgtattctga aagttcaccg
     2401 tatttataag tccttactat aaaaacattc tccgcaaaac aggtatatat cccaccaaga
     2461 ctccacccgc tatgattagg cggaattagg gttcgcttct tcgtttttgt cagtgtccac
     2521 ttgaaagcaa ttttctcatt aacgcctacc gtgtctttat acatttgctt ggcaagcatt
     2581 aaagagcggg tgatttctat aatgatattc tcaaaagcaa gtaccggttc tccccagtcc
     2641 cttggatcct tttttaaatc ttcgaagtaa gaggttcgga atataaaaaa accgttgata
     2701 aaaaaactaa aaatgtgatg ctgttgcgga tgaatataac acgaatgcca ttcgttcggc
     2761 catatttcga tatttttgtt ccttggttcg tctttcgagg aattgccgac catagggaag
     2821 ggaaggaagt tacgcacttc cagcggacga aaagaacttt ttagattttg ataaggaact
     2881 cttaccctgt ctggcaatgg cttcatcaag aactcaaaaa atccgatgtc cggaaccttg
     2941 ctgttttctt ctctattaat aatcattttt tatgcgttcc tccacgatct gtatgttctt
     3001 gatttcagca ttgaacgggt cttcttcttg ctgctgttgt gtttccgaat cttttgcttc
     3061 aattggtctg atgtgtctac aaggtcgatc tgatgaccat tcgatctcga tattgtttgt
     3121 tttcatgaga tctataatgg catcgaatga attctctagt tcctgtttat aaaatggcct
     3181 cagactgtca ttgtgggaag atggattgca tattctattt ttaagttctt tgaggtaaaa
     3241 gaaactgtct attccaaagt tatttttgat ttctgtcatg gttctttgca tagaaatcag
     3301 cgctgatcga ggatcggtag gatttccttg ctctgccttt tgaatggctt ctaaaatcct
     3361 ctgaacttga tccaatatga ctttgctctg attttttata tttgcgagat gaagtttctg
     3421 ctctaaattc ccgtctttgg caccaaggta tccatctatt tgtttttcca tctcttttct
     3481 taaaagattg ccgcactggt cgtaattgaa attatcgcaa gattggcggg ctttcatcca
     3541 aaaatcatcg ttgggtcgaa taaagggcct ctcgaacaca tggccgtttt ctttcactga
     3601 ttgagcatac atctcataaa aaaaccactt ttcggtatct tctacgcggt ctttgaacgc
     3661 ttcataccat gcccggtcgt aggtgaagaa gaatgtttgt gtgtccgcga aatgattttt
     3721 caatatgtca aaaacggcga gacggttgcc catgtcaagt ccgatgagta tgtcgtcgag
     3781 gactagaatg tcgagccgat ttggcataag agaggatagc ttcttaattg ccgcaaaata
     3841 aatagagatg gagagcgcag aaagccgcgc ctcgttcagg aattcatgat agtcgaccac
     3901 cggttcgcca aaaagttctg ctgtaagcgt aagcggatct cctacggcgt tacaagtgaa
     3961 accgttgata acgatgtcaa gtttaaaggt tgcaagaatc gcagagagag attccttcaa
     4021 atcagtattc aggacagttg caagatattg gcctttttct gcgccaagct ccttgagtaa
     4081 ttttccgccc ttttctgcga ccggataatt ggcgagaagt ttttcaagaa tcggcaaaag
     4141 attaatttca ccttgttcgc tttcgtcctt caggtatagg cgaagaatct tcttgtaatc
     4201 gagaaaagga gaaatagatt gagctgactt cagatactct tgtgaaagct tgtcgccgga
     4261 atcgggagag ctcagggtga gtttctgggg tatatcggga tttttgttta tctcgaaagt
     4321 cacgctccag tcttttgcat aacggtgttg cgcgtgtggg ctgtcgattt ttccccatac
     4381 taagtcatgc agtgctttgc agaacgaaga tttgccgcta ccgttctcgc catagaccag
     4441 aaggttctta ccatttattg ggattggcgc atgttcgccg taaaatgcgc gataattgtt
     4501 gaaagcaatt tcgttaattt ttgccatcgg cggttccttc aattacttta atgaaatcaa
     4561 tgttttttcg gttgataatc gctcgcatga caacattatt gtttttcaaa tcatggtata
     4621 gtttttcgca gtgggcaagt ttctgtgcat cagtcatgtt ttctttgaac ggacgggcaa
     4681 gtttttcgat aatttcgctg atgtgaatat ctcgtgctct tgtgaggtct tcaaagtaga
     4741 ggtcatagac catattgtca atgatggttt cgaagaaaga ggccatcaaa ggttggtcga
     4801 caacttttag gtgctgaata tagtaaataa gatttttgaa gacatccgcc ctttcctttt
     4861 cagcttttgg aatgggcata ttttgtaggt attctttttt gtatctaaat cccttttcat
     4921 caaattgtgg accagtatag aatttaacga aagagtaaac gccgagcctt gaagctagca
     4981 cggcagctat tatatgcaga gattttcccg ttagtataaa attggttgct tctgcataat
     5041 atccttgggt gtccaagtag aaatagggat aatcatcatg attgatggcg tctcgcggaa
     5101 gcctcataat atcaggataa ataatcttct ccttctcgaa atcttcaaca taggcgcagt
     5161 tgcgcaggtt gtagggggtg tcgcctttgt cgtagcgttt gaagatttct tctcgatact
     5221 gatctagatg ttttttgacg gcagaatagt tgttgatgtc taccggcgga atctggttta
     5281 ttgtggggac aatcccttgt ggttgcttct gattctggga acccacaagg ggcgccccta
     5341 cggaagcata tccgttatgg gcatttatca gccactgatc ggcgaattcg atgcggtatt
     5401 tcttgatgtc gcggccgcgc aggagtggtt tgatgatctc ggcgctcttg gggtcctcct
     5461 tgatcagccg gttttttgtc ttaccgtcta taatgaacgc ctcgttaaac ccggtcttga
     5521 tgccatagtt tatctgcaca ttccactcgc gaagcggcgt gccaagagtt tctatgcgtt
     5581 ttcttattat tgattcgtca cccgatgaga tgatccaact ttcagagctg agtcctgaaa
     5641 gcgtgaaact gtttttttca acataatcgg caagatctat cattgtgtta aaatcttttt
     5701 ctattttgca tgccttagct tttctttccc tattctcttt gtctttgttg gtttttgttt
     5761 tcatgaaaag cagaatgttg gtgtcaactg tcgcactgtc aaagacagca tatccaccaa
     5821 agtccattaa aatcacaggc tctgttctgc tggaaaaaaa atctctcagt ttttcaccgt
     5881 atttagcccg catccacttg ttcgaggtga tgtagcagag gtgaccgcct tctttcagca
     5941 gttctgcacc tcgctcataa aaaagtgcat agatatcgcc ggtccgagcg aacgagagat
     6001 agttctgacc ggcgtatttc tcggcgagtg cgccatgatt actctgcaac tggatgtagg
     6061 gcggattgcc gatcacgatg tcgaagccat cctttacgcc gaacatccac tcgggatcga
     6121 agaacggggc gggagcgctt ttgaacggcg accattcggc gatcagtttc gtatcgctgg
     6181 aatccttgac ccatgccgtg acgaacttga aaagctccgc ctgtttttgg gtgaactctt
     6241 ctttgtactg ctccttatct ttcccgtaag cggtgaaata gagttctcgc aggtctttga
     6301 ggtcctgtac ctgcggcgcc atatcggttg cgccggtttg caggttgttt agtctaatga
     6361 gggtgtcggc gcaaacgaat ttgaagtcaa gattgggaag cggatgtatt cctcgatttt
     6421 ctgctgcatc gtcaacccgc gcttcgatga tgagcgacag gaacacgcga agccgtgaaa
     6481 gttcgaccgc tattgtctgg ttatcggttc cgtagatgac ctcctgaatg agccccatct
     6541 tgtgcacata ctcgggtcct tcctttttca ggttgcaccg caccatcgcc ttaaattgag
     6601 agggtacgcg ggtcagcatg cggtcgagcc accgttcggc ccccggatcg atacggtcga
     6661 gaatgaggag cattttctgg agcatcccca tcgggaaggc gcccgagccg cacgcggggt
     6721 caagcacctt gagccggtcg agcgcctcga tgatacggag ccgcgctttt tcgtcgggaa
     6781 cgcagttgat ttctgaatgg taagaaagca ggcagtcggc ggtctcttcg gtaacgctcg
     6841 cggcctgtac gagatacctt ttcagacttt gattcaccat gtactcgact atggcgcgtg
     6901 gcgtgtagaa actgccggtc gcattgcgcg cggtgttgcc tgtttcgggg ttgatctcgg
     6961 caagcagatt ctcgaatatg cggcccaaca tttcagggtc tatcgaaagt tccacatcga
     7021 ccggcgtgtt ctcatccagt gtaaagttga atcgttcgag cagagcaaag agatcggaaa
     7081 accatttgtt gggaacgacg atgatgttgc gcgaactttg gctgggtact ccgttcatac
     7141 cggcgatata tttgtcgccc ggttccgggt cgaacaaacc accgttgaga aacggtatcg
     7201 tatcggcgaa ctgtccaatc tggagaagag aacgtgggtc gcgatcgttt gttggcgcgt
     7261 taagcacctc gaaaaatagc ggttccaata catcgtggta atattcggtg taggaatcca
     7321 ctatcttcga cgcgaggagt gcatcgggta agagtgcaat cccggtggat gatcttttct
     7381 ttttgaggaa ccagcagaaa atgatgcgac cgaggagcct gaccgcgaat tctttggtct
     7441 ctttccggtc atcggacagg tcgggataca tgacctgatc gtcgctgacg aaacggtgaa
     7501 acagcgcttc gatttcttgg tagaactcct tattgacctt ctcgaccgag aaggcatccc
     7561 ttatctcatc gagagaatca aacggcgctt ctccgatgcg attaaggaaa gtccgattgg
     7621 actcctgcgg gctgacaaag taagtgaaac gccggaaagg gccgaactct gctttggttc
     7681 ccttatattg gcgatgaatg agactgaagc gaaacaaccc ctttttatcg tggaacacaa
     7741 agatggccgc cgcatggaac gcgaaccgtt cctgcataat cttcttggcg agatcgaact
     7801 gcgccttgcg ggaagtgcgc tcggtaagac cgtcattctt ttcgaccgca acgacggcca
     7861 gtttgaaccg gttcttcagt tcgatttcgc cgatcaccgt gaacttgtca aacttgtcgc
     7921 cgtcgtaacc tcgcaagggt tccatcttct gctcgaactt tggcgacttc gaccggaaga
     7981 aaaaggccag ttcgtcgcgg ctaaaatcct tgacgatctt ttcgagaatc ttgcgggaca
     8041 tgttttttat tcctttggtg atttttgatt ttcaacggcg ataatgattt cgctttcaaa
     8101 gtcgcgcacc atcttttcga ctgtctcaag atacttctcg ccgagttctt tggcgagttc
     8161 ctctagaact tttctgacct tctccggctt gtccatcggc atatcggcga tcttgcgcaa
     8221 agtgtactcc gaaagagtct tgtattcatc gatatcccgg tgcaaggtcg tgatgaaggg
     8281 acgatacggt tccaattccg gcgagaggga cccaaggatc gaggaaaggt tgttggacgc
     8341 tttggttatg atactgttcg ccgtcgttcc ccggtgtttc gtttcggccc cccggatcag
     8401 gtcgtaatgc cgccagaaat cgggaccaag ttctttcgac ggtgtttcgg gttcacatgc
     8461 aatcaaggga ataacttctg aaaggggcaa ttcttcgacg gtatcctctt tgcggatgcc
     8521 gcgcacaaag aaggcgcgcc ctttgcgcgt gaagaccagc agactaccgt cctcatgctg
     8581 tttcgccgtt ttcacacgtg gcgagaactg cttgatccgt tcggtcgttt cgggatgctt
     8641 ctccgccaag tcaaagtagg tgcgccggac gaaagttgcg aagttctctt cttcgccttc
     8701 ttccgggttc ttgttgatct taacgaacag gctgctcggt tttacttctt cgtcgggatc
     8761 gaatatccgg gaatcctccc ccagcgcatt gtggatcatg aacatcttct gcgccgctat
     8821 ctcgcgcgac ttgacgatct ccgctccctg ttcggtgggg aaaaagttgt agatgtggag
     8881 ttccttaaag atcttttgtc cgatacggtt gatgcggccg actcgttgaa taacccgcgt
     8941 cgggttccaa gggatgtcat aattgacgat agcgcccgct cgggcaaggt taaagccttc
     9001 ggacatcttg tcggtagccg ccagtatcat gtgatcgtcc gcttgctttt tggacgaggc
     9061 atcgaagttc tccagtatct cttggacacc cgattcgcgt aggtccttgg gcatcacgag
     9121 aaccgtgtcg gggaattcct tggtcaactt ttcgcctaga taattcagtg tgtcgacata
     9181 ttcggtgaag atcaccacct tgcgttccgg ttcgtccgac agcttttttc cccgtatctc
     9241 aggcaggtgg gctatgagcg acgccacctt ggggtcttca gcaacaaggt tgagatcgcc
     9301 aatctctttc agtatttttc tgaaaagcgc aagatcgctg tctatatggg caaaaaactc
     9361 ctcgcgccgg acaaaggtgt ttgtctggta gatgcgtcct tttaactttt tccccttttc
     9421 ggccatctct tcggcgaaac cgagaagcgc ttcttccatc tcgtcgccgt ctaattgaga
     9481 tatctgctcc atgttgtggc ggtcgaggat gtatctgccg tcagtctttt ttatgaaggc
     9541 aaggaccaac tcattaatct tgatgaaatt ctgcaccgtt tgtcggaacg cggcaaagga
     9601 actctcgaat cgcttaacag cgaggcgtcg catgaagtct ttcaagttct tctgagattc
     9661 gccctccaat gtcttcttgt cttcatcttc aggattttct ttttcgtaaa ggtatggcgt
     9721 gtagatcgcc ccttcaaaaa atccctcttc gccaaagtac tcgccgatta ccgtgtcgta
     9781 gaagcgcgat tgctcgggtg tgagagcaaa aaacagttcc ttgggcggga ccgtctcgga
     9841 taaagcgcct atctctcttg aataaaccgg gtcattcttg atgtcaagcc ggttccgccg
     9901 gaggacaacc ggactgatga cctccttgat ggagttcgag agtagcctgg tgcgttgctg
     9961 gacctctttc aggtcaatgg ggggctttct gcctacaaac agtttctcgt attccagttc
    10021 cgcctttttt ctcttttggg catccttgct gttatgattc tttacaatgt aggaaaggcg
    10081 gccgtaaaga taatcataag ttctgaatcg tgcctctaga tcgtcgtcga gcgtaatccg
    10141 cgatttgccg gggatgatga agagtttcaa gagggaaaat atgtcggcgg gcgagttgtt
    10201 gaacggcgtc gcggtaagaa gaatgacgat acgaccgcga caaatgttct taagcagttc
    10261 gtaggcgcgg gtgttctcat ttctgaagcg atgcgcctcg tccacaagca ccacctcgaa
    10321 atctgggtgt tcggcgacga attcgaccgt tttctcaaga tctcccgacg aacggatctc
    10381 ccaaccatgc agttcgaaat cctcgtaata ctttttccaa ccgactgtct tcttgtcgtc
    10441 gccgataagg ccgggaggac agataatcag cccacgccgg ttgagtaggc gagcaacgag
    10501 tcccgcaatg atgcttttac cgaggccaac aacatcggcg atgatcaccc cgccgtagtt
    10561 ctgtatggtg gtgagtgact gagcgaccgc atcggtttga caggaaaact cttgatagcc
    10621 cttctttttc agaagttccg aacaaggcat tttcttgtcg atgtcgatga aagtatcaag
    10681 ataggtcttt aggataagaa gatatgcctc gaagggggtc agtttggcaa caagggtgtt
    10741 catctcgata aggtcgagaa gcctcttgcg gatcggctcc tgttccgtga tcgggacacc
    10801 tcgcgcccaa agttcgtcga agtatttctc cgcttcggcg gtcccgaaat cgccgatctc
    10861 gatgttgaac tcgtgttgac cggtcagccc tgccctcgtc aggttgctgc tcccggtaat
    10921 gaacttgtct ttggcgaggc cttgatattc tcccttcgtc ttgaagtagt aaagtttcgc
    10981 gtggttggga tcgaaggtgc ggcgaatgtg caaccgcttc gattttatca gttcaatgta
    11041 gtagacgctt tgctcgtagt attctttggt gtcgaagtca tcctgccgaa gccccttttt
    11101 tatcgattca aaatagcgat caactatctg cgcgacactt cggtcgttcc gggcaacttc
    11161 gacgaccatg tttgatgcaa gcgcgtcggt gtcgaggccg acaagtatct tgagcgttac
    11221 cgaggaattc tctttcagcg ccggaagcag ttccttgatg cccgaaaagt agaaaaaccc
    11281 gacgaggaac ttcaactctt cgctgaagcc gatgagttcg ttcaaccgtt tttccagttt
    11341 gttcgcgccg ctattggtga tgaacatatc ggccctcccg gtgggtcagt tgtcggatat
    11401 tacgacttcc ctataaggaa atcaaaatta ttatggtaat agggcccgcc gtcgcaggag
    11461 ctagggcgga cagaggaggg gacctatttt gtccctgtga ccgggatgaa cgatctcaac
    11521 cgcatccggg tgaagtcagt tctgacaatt ttctttgtcc gaggatctaa aaaatattcc
    11581 ttctcttttt cgcggagaaa gacaacccaa tgccaagtct ctcgatcctc attgtaattg
    11641 atagcgacaa tcgctttatc cgatagggag gagattttcc gaactaatcg tcctttttct
    11701 gtcttgatct tcagaacaga aagcaatgtc ctcaagtcgt tgctgtctgt ccgaaagttg
    11761 ctctctgttt tttgccaatt aaaagcatgt cgagcgagcg agaaggtctc tttgtatgag
    11821 atccccgcta tcattgctac acaagcaagt ccgcagccag ttctgtgttt ctgacgaaca
    11881 agtttcatgt tctgcttttt ttattcagtt ccagaatgga cggggctttc ccttcccaaa
    11941 agttacggat gtcgtctttg cgtttcttca agatcgtaga tagattcttg taataagaat
    12001 gattacggga aggatggggg atagcaataa aaggtctttt taacattgca ccaagaaaat
    12061 tgcgggcctc gttgccgagg gccactatgc ggtagttctt cttctccgtg aggagcgtga
    12121 tctccccgtc gagatacttg cgacaggtcc ggaaagtccg caatatgtcc atatcggtat
    12181 gccttgttcc gtcctgacat ttcaccagat cggtccagac acaattgcgg ccgaatcgac
    12241 ggttgggagg aaggagagcg tcaagtccat cggcaacggc cttggttctt tcatagaact
    12301 tcgtgtcaag cgacccgcag taatccagaa ccgcctcgta ggtgagacct ttttcgatgt
    12361 agagtttctt ctctagcgga cttgccggcc ccgggttgct accgatgaag aggatgccgt
    12421 tgggcgcctc attgatggcc ttgtcaaaga taaggccgcg cggggtgata ccctgtgatc
    12481 gggctttttt gcatgacccg acaaagccgg ggcaggtttc cgaacaggca acgatctctt
    12541 cgccgatggc gaagagttgg tctttcaggg aattggtggg catggagttt ttcctgttta
    12601 tctcgatttt ctgaatttct ttccctcaaa tatacctttg gctatctcat acacttgatc
    12661 cgcgtgttct ggatcccatc tcttttccgc tattagataa ttcttcactt ctatcgggtt
    12721 atataatgtc ccttttgtct tcatttcttc gaacatgtta atagcataat ctctatcaga
    12781 tggatgagca agtcctgtgg atacattaac cgtgtcatgg aggttatcaa gtcgtgtttt
    12841 aacaaccgca gacaaaactg taccttggct cttaattcca ttttctatat cagttgcacc
    12901 ccaacgtggc cgccactcag atatatcgtc ttctacccaa ggtaccacaa ggacatctgt
    12961 tacatttaat atactatcta ttttatccaa tagcttttga gtcgcataaa tagcaattac
    13021 agatccttcg aaaacattat aatggtcctt cttctccgtc atcaatgaaa actcatggcc
    13081 attcgcacca cacctattat tccggcatat ttctttaaca aaggcttctc caaatacatt
    13141 ttttatagct ccatccaaat tatctttttg tggaacggct aaaagtattt tctttgtagt
    13201 gattttgcct aaaaacaggc acagtgccgt tttcagctta ttctcatctg gacctgtaga
    13261 attaatgtaa taacagttct tcatgagcct tcctccttcc ctttccgttt tgatgttgat
    13321 cgaacttcct cttcttcctt ctttttctct ctgtaatatt tatcaacata tttttcatat
    13381 tctttgctaa atactattcg cacattacct ctacttacaa gcttctgcaa cattcctatt
    13441 gatttaagaa ctttaatact agcagctaat tcctcccgtt cttccttcgt atgcatattg
    13501 cacagtgcca agataacata gggctcattt gaacaatgtt tgatcaaata tgtcgctgct
    13561 ttcttggtgg caatcttttc cgattcatcg gttagatgtt tttcaatata cgaatctgct
    13621 gccattatgc gatatggcat ggatatgtaa tgatggaact ttctatctat atcctcttta
    13681 ctaaagccca tttctgcaag aaggcatata tcaattagaa ttttcatttt gttcgttttt
    13741 tcaaccattt cgttaagact cattctttct tgatctgctt tttcttcgta atgggtgaga
    13801 taatgacgcc catttttcgc tctctcacag aattcttcaa cagcataatc tgaaaacagt
    13861 tctatgttgt acctgtattt cccaactatt tctagtagtc tttctttcaa cttcattgaa
    13921 ttctgacgac ttgtttttct atgatatacc tctaaacaac gaacatattc taggaattta
    13981 cgttcaacaa atccggaggg aatgtatttt tcagcaatgt agttgttggt aaagggagaa
    14041 aggtctccca gcgaaagatc aatccatttc tcgataaatt gcggagcatg ttcgtgaaca
    14101 tccgtgaatt cgaaaagaaa gtgagaaact tctttttctt ttgttaagga acccctgatg
    14161 tgctgtcttg aataaatgcc tatcggcatt tctcctgtgg caagcgtata gccggtcatc
    14221 tcgttcgagt atacaggact gtctgcggca aaagccagca tgttcattaa tgcgtgtgcc
    14281 tggctctcta tgtcgataaa aacaacctct tcttcatagt ccagccgaag agatacttct
    14341 tgtttgagct cctgtttatg agaggaacga cctgatgaaa tctggacaac tttgaagatc
    14401 aaataacagg tgcaacagtc ccatttcccg agtttcacat cttcgggttt tttatagaag
    14461 atggctgatt ctcccggtct cagcgttatg tcttcacgat gggtgaaacc atcgatattg
    14521 acccattgac tgagtgtttc tgactgaaag tctatccgag aaaaatgagg gatatcttca
    14581 aaatgtactc cccaaaacaa atttctgctt tcaaattcat attctctcaa tccaatcaat
    14641 ccaaatcgct gcaaatagca gtcgtataga gtgattttat cgttgttgtc ggtcaagccg
    14701 agtataatat ctgttttttt tatcaaaggg agatttgacg gcgccaagaa caaccgcgag
    14761 cctttttctt ctgaaaaacg tagttccccc cggacttttt cttgttcttt tccggggaac
    14821 caccaatctc ctttaacgac gaggtctttc atgtggcatc ttttctctgc ttgttctgtt
    14881 caaaagagcc ttgactcttc ttcgattgac tcttggcgaa cagcgtacaa ctgttgtggc
    14941 tagtcacctg ccgcctctct gcttttcgcc tgcgtcctga ttctttcaag gagatcaagt
    15001 gccattttcg tccatagtga agaagaacca ccggggtatt ttgcgtgttt ccttgcgctg
    15061 taaaaaatag aagcccagtg agctatttcg tatgccttct ccatcgcgta atgatcgcaa
    15121 ccgcttagtt ggcccgcgag aaagtgtatt cgacggggta tagattgatt gggcgttggt
    15181 gtcggacact cattcaagac ggcctctatc ttggccgcta ccttgattat cttctctgat
    15241 cttttcatgt ccatgacgct ctgctccgtt gttatgtcaa tcaaccgaag gatctacttc
    15301 gtcccatacg gcgattgatt cgtcggttta agggaaagac ctagatcact tgctttggct
    15361 cgcacggctg cttccgttct ccccaatttt aagccgataa cccttgtggg cgtgttctgt
    15421 gccgctaaca tccgaaggta aaccatctct tccggcgtcc aagaatttcc ggtgtttcgt
    15481 gtcgatttgc tcattttact acctcactat cctatgcttt tgcatgcccg tttcctctcg
    15541 gtgaaacggg gggccgacaa aagccaaaat cctgttcctg atatccaatg gtagcaagca
    15601 tatttttcaa gtcaaccctt tttacatttt tatttttcta tatgttgtat tgaattcgct
    15661 catttttgct ccgaccatac catatatcgg gggtcaaaga actcgttctc ttctctcctt
    15721 caccccataa gataccggtc cccccgaaaa ggattctttt tttgatgggg atcacgcaaa
    15781 ggatgggaaa atctctcgtc cccccgaaaa tatgtatatt cggggttgat ttctcctaaa
    15841 aggtgtttat tcgtcagtat attaggcagt taaggtgact gtctgaaggt gaaggagagg
    15901 aagaatgggg aagagattgg ttcaaagcga tcacgacttg atggtggctg cctatgcaaa
    15961 agatttgatg aaattaggct atgaggtggc cgtggacttg cctggcctta tcacgccagc
    16021 gcaaataggt gagcatattc cggacatcac cgcaaaagac cccggcactg ggaaatttca
    16081 tgtttttgaa gttgagacct ctgattctat caacgacgaa cataccgcta gccaatggtt
    16141 aaccttcgct gttgcggcgg ctatcggcaa ctctcaattt catgtcatag tgcccaaggc
    16201 cgcgtatttg tctgcctgtt tgcgagtgaa agcattaacc ctcaacaatg tcgatgtaat
    16261 cggcttttaa attcccccgc ctacaaccga gggtccggcg tcgctatggc tatgccggac
    16321 aaggcatcaa gaggccatct gtgactattc aaacgcgagc acctgtttga cccgccatag
    16381 cgtactcgcg gaggcgggga gttgcgcagc gtcctcttga tgcaccgatc cggccctgcc
    16441 ggcggttgtc ccggcgggca ttaaccggtt tcttctttcc cccctttctt ctttgccgct
    16501 acaaagaaga aagggggtcg gcgaagagca tcgcgctcgc cgaaactctc cactcccccg
    16561 aagggctaat cctccccccg cagggctctc cctccttccc caaagccgcc cgctagggcc
    16621 ttcctttccc ccgtagggct atccaccccc cgggtcctcc gccgacctgc ccgccgcacg
    16681 gctttcggca ggcgggaggc tttgggccta cgctgcggac tatggcccac aggccggccc
    16741 gcaggcggac atacgcagga cgcccccctc cgggggcggt tcggccaacg gccaacggca
    16801 gcgaccagcg aactcctgcc ttcgcctccg gctatggcga ggcaagccgt agtacgccgt
    16861 cagggggcca aaacccatct atcccttctt cctaaagaac tcatccaacc acctcctccc
    16921 cgcctcataa tcccccttcg caaccgcctt cttaaccgcc tctgg
//
LOCUS       DAOQUE010000004        16603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_3958_length_16603_cov_4.848320, whole genome shotgun sequence.
ACCESSION   DAOQUE010000004 DAOQUE010000000
VERSION     DAOQUE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16603
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3958_length_16603_cov_4.848320"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKH10_00215"
     CDS             <1..362
                     /locus_tag="PKH10_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26575.1"
                     /translation="LTTDHGAFYLACDGYEYRFSGRPDTYLDYFPLNGDMREMAENHL
                     YNGWVYIPDGSTVKISTNNAVPSYCGNGIVEPGEVCDGNTVYCEDLDSTQWNAGTANC
                     NSTCTGYNTGDCYWSGC"
     gene            365..2107
                     /locus_tag="PKH10_00220"
     CDS             365..2107
                     /locus_tag="PKH10_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26576.1"
                     /translation="MRHIIITIGGGAAAPILVLFLLVACNPSKPAPTDDATTLCVGRC
                     GVVNGAQCGPCEYGYTCNEAQYCVEIAGNDSDGEEDDLSVEMENDDYDLSPDTDFVAT
                     SDTDGNPWENIDCAEAPEPWKSDDDPYADRDILYRYYGDFDVVVKDPEGVRNLWAEKF
                     TGLDGGTIIPCNCFWQTPTDACADNFPFEPVITEISPSAPTQSPKDKVLTAFVCDELL
                     TPPMEWQISWVYGRDPKYNARNGKVLFPMRSPHAMGLYGGHHAYLLDISKRQLLAVTT
                     GYSNAYFNGRYAFMTIRDMEKNYDFPDKSGYESVPWSEFIYYDSVEHKYGYAWYGEKF
                     YFARSIHASDTHIIFSYQKLPDTNEGSRTFYARIGEWNTWKELTFMVGSSIGVVDAGF
                     PSMVGQYVAFYNWDVEVAFCDLEKGDAGCFKVSRDNENGRMPKILDNKAVYYAAENKT
                     TKARSLVKADITNPQDITYTTLVTHEKVLTPDDIDKDFLLYGKEIGERSNGDPLIANC
                     FYRFSDGKSFCMDDPGHDAKIPTDYSYLPGGHYVVYQSWYDLVLRDMECYCDQFPSRC
                     PYNDYTPKPRPATK"
     gene            2194..2886
                     /locus_tag="PKH10_00225"
     CDS             2194..2886
                     /locus_tag="PKH10_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26577.1"
                     /translation="MKPRDLLFVAGVAALFSPFFVSDAVFDGYKMFNADHGIIMGAIK
                     FAILATLGEIIGLRIRTGEYDAPNFGILPRAIVWGFLGAAIAYAFIVFPSGTIKFLAY
                     LGLPVTPDTLKGAVTGMQVATAFSISVGMNLIFAPWMMTLHKITDEHIARTGGTVAGL
                     FRPIAFGDILSKMNWGVMWGFVFKKTIPIFWIPAHTVTFLLPEQYRILFAALLGVVLG
                     VILAIAALMAKK"
     gene            complement(2897..4270)
                     /locus_tag="PKH10_00230"
     CDS             complement(2897..4270)
                     /locus_tag="PKH10_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNT26578.1"
                     /translation="MGRYPFFLLALLFAFSGCDVETCVGEECEPCYKKECGEDQGHSC
                     GTCYSSYEYCSTNNQCVLYEDAPECGEFECGDATAETFYGSQTISCGTCADDSYCSSG
                     QKCVGPLWRLTVDAKFEGQMPCIGNDGTLYVPSSGGYLFAVSPEGALKWKTSGIYDNF
                     PVIGNDGSIYVLVNGELSARDPVDGSEKWRFVPEKKQYGGTLSSAVANDGTIYMSDGV
                     YVHAVSSEGALKWSFSYLENEVFSVIVVGSDDALYVAYVGGMAEGYPYYRIVAVTAEG
                     EQRWVSSDKYDHIYASLVIGRDDTVYIGGRLLSDPPDEVSLFALGVDGVTKWKGPSGI
                     PMIIDEKDSIYACDYSNTHMVTVVAPDGKEEYSFHLWTGDLTLGDDGTLYNSGPDAYD
                     LEGNELWAIEAYSGFFPVMDDRGVIYFGNNEASSIYAIPSLSTGPADAPWPMFRGSAK
                     RNGRVNR"
     gene            complement(4329..6065)
                     /locus_tag="PKH10_00235"
     CDS             complement(4329..6065)
                     /locus_tag="PKH10_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNT26579.1"
                     /translation="MMERFFGRLAAFVVPAVFLISCGDESPADAVPADEMRVAMTLLA
                     ADDTACEDYVGDNIQDKLGALTIRIYDGSNPKPRHTTSLTKDQLSGDVTISGIPDVSN
                     ARLELYGFESISDPAPKWVGTVQGLNFVSGKKTNVYASLYPVSGIGCFPKKLQTPRFG
                     HVATTLPDGRILITGGFTLTNNGTWDADDTVELLDVESGTVDQIADMKEFRAFHTVVP
                     LPDGRVLIAGGVSSMKMEKTSVTDYPDLPVTLNGAPAGMEIYTVGLPRKNQRLTTTDN
                     TTVDWVEGALDWPSIFAPYQGYAFVQTDETTGSGTLFVVGGAKDGAAFSKIYGIEVTV
                     GEDGTISAAVNEYHSDKTETFVMPVVAPAGLDNGTPRVLVIGGRKATSTAKFTHIISK
                     DVYEEWNDDTPNLFFGSAAAQGDIIVAANGLDLPKDATAFEMHARGYRFNMADRTYRS
                     GDIFPKSTWFHEVTLDVTEGYNGYFSVIGGAEGFDTTQTSVGATNFYQLIDLDMMYWE
                     NDWDPAQHDGAPAPRFGDYFNGSSESDPGRALHRVARSGNLLYITGGLDDDLKSLTLV
                     GTIKLMPTTRPW"
     gene            complement(6149..6679)
                     /locus_tag="PKH10_00240"
     CDS             complement(6149..6679)
                     /locus_tag="PKH10_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNT26580.1"
                     /translation="MNTEKNGHPPDERRILVVSDTHGSLPDRICDIAADTVLHAGDIG
                     DDRVLAALHCFDTVHAVRGNTDTALPELPDTVRADIAGVRFFLVHNLTAPHRLLPENR
                     REIAGSRPRLVVFGHTHQPLLREEEGILYLNPGSLGRPGVDGTPTYAVVTVAGGAVRK
                     VEICDAATNLPVFSWP"
     gene            complement(6744..7757)
                     /locus_tag="PKH10_00245"
     CDS             complement(6744..7757)
                     /locus_tag="PKH10_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT26581.1"
                     /translation="MKRILVLGAIALLFVACGGKTTQQKQADRNLALLEENTADPDFV
                     KGRSLVMEEKYGEALPILEKSADPEALFFRALALQGLGKADDAAGLFAVCIQNGIQPA
                     ESLYNLGLIAYETDDKESAAARMNDVIAKEPGHAGAHYFLASAAYEKNDMATAETHFR
                     AAAKSSPRNGAAWEGLFYALVSQDKFAEGWELRDKLDLATGDILANLLLVGEKLGKSA
                     DALALIPTGVPSDAVKLQRIVLIAQTSGLAAAIAEADKLPKELTAQAGFTVLDRGVEG
                     SLEWLVSKMDDGKVMLTCTASKEAKPALHELAVTAAGVTVDKKNYPADTFRAAIPGIC
                     NIQ"
     gene            complement(7754..8323)
                     /locus_tag="PKH10_00250"
     CDS             complement(7754..8323)
                     /locus_tag="PKH10_00250"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNT26582.1"
                     /translation="MRSIAVFVLSCCLLFSCTKKEEPVAPVVTQPKMPEALDEVDKAL
                     ERELTDEDFQKGKNLHGIVMRSLITGEEVKFSDHKGKKIIVDFWSSWCVPCIEMFPDI
                     ERIKKEYEEAKGLVKIISISVDPLAANARKIVKKQGVTFEVIQAPESLQNAGILLPFT
                     IFADENGVVKETTNGKHSYAEIKKMAGLQ"
     gene            complement(8333..9358)
                     /gene="purM"
                     /locus_tag="PKH10_00255"
     CDS             complement(8333..9358)
                     /gene="purM"
                     /locus_tag="PKH10_00255"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNT26583.1"
                     /translation="MSGDAYRKAGVDIEEGNRLVPMIKELVKKTHRPEVLAGIGGFSA
                     MVTIPKGIKKPVLVSGTDGVGTKLKLAFMAKRYDTIGIDLVAMCVNDVLVLGAEPLFF
                     LDYFATGKLSADTAALVVKGIAEGCAQAGCALVGGETAEMPDFYPPGEFDLAGFSVGV
                     VDKDKIIDGSKVKAGDVLIGIPSSGVHSNGYSLVRRILFTQQKLKINTRIPELGATVA
                     DAFLKPTIIYAKLIKKLLKKVPVKGMVHITGGGFYDNIPRVVPEKLAVEIGGDFTIPP
                     LFQWLKKAGGVSEHEMFTVFNCGVGFVCVVDKKHAAEAAKLTGGHIIGTVVKRARGPV
                     IFKKKYF"
     gene            complement(9496..9798)
                     /locus_tag="PKH10_00260"
     CDS             complement(9496..9798)
                     /locus_tag="PKH10_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT26584.1"
                     /translation="MAKKIVAATKNDFIKAIKENMAKEINLTNDQVKALYETFVQIVK
                     DTVNAENKLVLSGFGTFKKRNLKARKGVNPKTGQKIQIKASKTVSFKAAGAFKKAL"
     gene            complement(9917..11011)
                     /gene="add"
                     /locus_tag="PKH10_00265"
     CDS             complement(9917..11011)
                     /gene="add"
                     /locus_tag="PKH10_00265"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNT26585.1"
                     /translation="MQGARVSVAAPRPFDRDFFRSLPKTDLHVHLDGSLRPLTILELA
                     ERQKVDLGARDLDGLKHLIGPGKVHASLNDYLKGFETTLKVLQEEEGLYRAAYELAED
                     SAKEGVTYLEVRYSPVLHTQKGLPLTTIVESVLHGLRDAKRDLGILSGVIICGIRNIS
                     PAVSLRMAELAVAFKNKGVVGFDLAGAELNYPARDHLGAFSLILSNNVNVTIHAGEAY
                     GPDSIHQALHYCGAHRIGHGTRLREDGDLLNYVNDHRIPLEVCLSSNAQTGAVKRIED
                     HPFPFYLDLGLRVTLNTDNLLITDTTMTDEYLKAYRLFGLAPQQILDLVINGVKSAFV
                     PYHQKRDMLRAAKKKTIDLINSYAGTTIIC"
     gene            complement(10993..11649)
                     /locus_tag="PKH10_00270"
     CDS             complement(10993..11649)
                     /locus_tag="PKH10_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26586.1"
                     /translation="MTRRLCLMLAPLVLLICAACSEEPKDDIGKYDYLNDLTSYYNDD
                     VYPEYPDEDAPPEKVWQARFQFYGIINAENTPTADLQFGDGRLTYLNEALEETVYIGA
                     LWVMKKNINNSAGFSIPLLQVFFTDHLQDGEATTFVLQLDGSSMTKKEVFYLNNDKLY
                     LTETTRTDGEIAQICFEREALEGRVNLFSSSIRDGEPLHLDGYADLRVMDPVECKVLE
                     "
     gene            11804..12205
                     /locus_tag="PKH10_00275"
     CDS             11804..12205
                     /locus_tag="PKH10_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HNT26587.1"
                     /translation="MTNERFQERFAELQKAFSRLEEALAVPVTDAVIMDGVIQRFEFT
                     FELTWKTLKLLLDHKGLATVAAPRDVLQGAFAAGIVSDGEAFMQMLRDRNLTTHIYKE
                     DMAAQIYDRIKNAHANALRTLIEHLAAEKIA"
     gene            12202..12492
                     /locus_tag="PKH10_00280"
     CDS             12202..12492
                     /locus_tag="PKH10_00280"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT26588.1"
                     /translation="MNFGLPDKVLAEIRAVLAKYPLAKAVVYGSRARGDHRPNSDIDI
                     AVWPKEGATDRIAMLAAELDGLDIIYKIDLLEMRPDTDPTLAAKVARDGREL"
     gene            complement(12535..13887)
                     /locus_tag="PKH10_00285"
     CDS             complement(12535..13887)
                     /locus_tag="PKH10_00285"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNT26589.1"
                     /translation="MRKALTALSLVSFAFFLYGNDWPDTIVNGQETDYAKYQGVVALI
                     MDTTAGMGLCTATLIDPEVLLTAGHCVYNREGDDLILDAVSDPSLLTIYNGAKISSSP
                     SIAQATQVIKHPDWDGYLETLGYKDDLSLIKLDRRITELEIYGLRQEPQSEVGDTGVI
                     VGYGVTSDTASDSGVHRWGTAEILEYWNSAFGKDLVVGNPSSGCFGDSGGPFFTVQNG
                     RTVIEGVTSYGGDCSPEEGGHYMPTLAFMDFINEAFQSLTGHGLDQICGNGIRESGET
                     CDGDSVDCGILGPYASGTSAPCNNDCSGYLTTVCNEVICGDGIREGTEICDRDSQSCD
                     KFGDYADWLWAPCQSDCMGFDTTYCWDAAKATCGNGMLEGLEECDDGNLNDNDDCTAS
                     CKNAYCGDGIINTLREECDWAEKTYQGFFCTADCAKAFSDDQDENNSGSGGGCSLSTL
                     "
     gene            14058..14441
                     /gene="queD"
                     /locus_tag="PKH10_00290"
     CDS             14058..14441
                     /gene="queD"
                     /locus_tag="PKH10_00290"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNT26590.1"
                     /translation="MRVSVYREATFSAAHHLREYRGKCENLHGHNWRVRLYVGRDELT
                     ADGFVMDFKEIDAALARILEPLDHHDINTVPPFDTQNPTAEHIAHYIFERAGAEIARR
                     DPLCRVERVMVWESDRSCAIAERDR"
     gene            14438..14869
                     /locus_tag="PKH10_00295"
     CDS             14438..14869
                     /locus_tag="PKH10_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNT26591.1"
                     /translation="MSYRRNVALVVENDAGLVLMGERSDIAGAWQLPQGGIEKGEDWQ
                     QAAWRELEEETGLTAAAVTIVAHAGPFRYDLPRRSKRGFRGQEQTYVLFRLTDTAAAL
                     RPGVEFRSFRWMMREEATAAVIDFKRPCYEAAFRAFRDRPA"
     gene            14896..>16603
                     /locus_tag="PKH10_00300"
     CDS             14896..>16603
                     /locus_tag="PKH10_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNT26592.1"
                     /translation="MRKKGGAGRTFRAVVIGLAIFGIIALIGAIFGYRYLKSQIPHIE
                     TIADYKPAQGTKVYAYDGQLIAEFYKENRRNIVPYDKIPCLLALAFVAGEDGEFFKHK
                     GIDPVAIMRAAFKYAFTGVKQGGSTITQQLAKAFVGTERTYTRKLKDMLLAMELERAL
                     TKEEILYLYLNEIFLGSGAYGVESAALTYFSKHVWELELAEMATLAGLPKYPSEASPK
                     NNPEKAKKRRDYVLGRMRADGYITQEQYDAAAATPMTVIPMKEIFLDKAPYFSEKVRR
                     HLQTAYGNDRLYDEGLVASTTVDVRASQILDESLYTGLRALSRRQGYTGPLYHIDLEK
                     DRDRYLERHRRSFGDISDLTIRRGTLYQGVVLDTAKDKATVQVGAVKRPLHLKHMQWA
                     RPHNPLGGWANIASTDQVLKPGDVILVRATDEQGVIEELDHRRTDLPLPRNFFFALEQ
                     LPVPQGGAIVKDPYSGYIRAVMGGYDFEQSEFDRTTQACRQPGSAMKPIFYAGALEQK
                     DADRKFRFTAATMLLDAPVAITDLNFKPENHDETFKGEVTLWEAIVLSMNAPAVRMFM
                     DIG"
BASE COUNT         3411 a         5018 c         4779 g         3395 t
ORIGIN      
        1 aactgacgac cgaccacggg gcgttctacc tcgcctgtga cgggtacgag taccgtttct
       61 ccgggcgacc cgacacctac ctcgactact tccccctcaa cggcgacatg cgtgaaatgg
      121 cggagaacca cctgtacaac ggctgggtct acatccccga cggaagcacc gtgaagatat
      181 cgaccaacaa cgccgttccc agttactgcg ggaacggcat cgtggaaccg ggcgaagtct
      241 gtgacggcaa cacggtgtac tgtgaagatc tcgactcaac ccagtggaac gccggaaccg
      301 ccaattgcaa ttccacctgt accggctaca acaccggtga ctgttactgg agcgggtgct
      361 gacgatgaga catatcatca taactatcgg gggcggcgcg gccgccccca tactcgtgct
      421 cttcctgctc gttgcctgca atccctcaaa accggccccg accgatgatg cgaccaccct
      481 ctgcgtcggt cgttgcggcg tggtgaacgg ggcgcagtgt ggtccctgtg aatacggcta
      541 cacctgcaac gaggcgcagt actgcgtcga gattgcgggg aacgacagcg acggtgaaga
      601 ggacgatctc tctgttgaaa tggagaacga cgactatgac ctttccccgg acaccgactt
      661 tgtcgcaaca tccgataccg atggcaatcc atgggagaat atcgattgcg cagaggcacc
      721 ggagccgtgg aaatcggacg acgatcctta tgcggacaga gacattttgt accgctacta
      781 tggcgacttc gatgtggtgg tgaaagaccc cgagggtgtg cgtaatttat gggccgaaaa
      841 attcacagga cttgacggag ggacgatcat tccctgtaat tgtttctggc agacacctac
      901 cgatgcctgc gccgacaatt ttccttttga gccagttata acggagatat ccccttccgc
      961 tccgacccaa agtcccaaag acaaggtact caccgccttt gtctgcgatg aactcctgac
     1021 acctcccatg gaatggcaga tatcctgggt ttatggacgc gacccgaaat ataacgctag
     1081 aaacggcaag gtgcttttcc ccatgcgatc cccccacgca atggggctgt acgggggaca
     1141 tcatgcctat cttctggata tttccaaacg acagttgcta gcggtaacta ccggctattc
     1201 aaacgcatat ttcaacggtc gctatgcctt tatgacgatc cgagatatgg aaaagaacta
     1261 tgactttccc gacaaaagtg gttatgagtc cgtcccgtgg tccgaattca tttactacga
     1321 ttcggtcgag cacaaatacg gctacgcatg gtacggggag aaattctatt tcgcccgttc
     1381 aatccatgcg agcgacaccc atatcatttt cagttaccag aaactgcccg acaccaacga
     1441 ggggagcaga accttctacg cccgtatcgg cgaatggaat acatggaagg aactcacctt
     1501 catggtcggc tcatcgatcg gcgtagtaga tgccggattc ccctcaatgg tggggcagta
     1561 tgtcgccttc tacaactggg atgtcgaagt tgccttctgc gacctcgaaa aaggcgacgc
     1621 cggttgcttc aaagtgtcac gagacaacga gaacggacga atgccgaaga tacttgacaa
     1681 caaggcggtg tactatgctg cggagaataa aacgacgaaa gctcgttccc tagtcaaggc
     1741 cgacatcacc aatccacagg acatcaccta cacaaccctc gtcacccatg aaaaagttct
     1801 gactcccgac gacatagaca aagactttct cctctacggg aaggaaatcg gggaacgctc
     1861 gaacggagat ccgttgatcg cgaactgttt ctatcgcttc agcgacggca aatcgttctg
     1921 catggatgac cccggccatg acgcaaagat cccgaccgat tattcctatc tgcctggcgg
     1981 tcattatgtc gtctatcaat cgtggtatga tcttgtcctg cgcgacatgg aatgttactg
     2041 cgaccaattt ccttcgcggt gcccgtataa cgactacaca ccgaaaccgc gaccggcgac
     2101 aaaataaaga aacgccccta ataacggacc ttttgacaaa tggtcggcag tttattagac
     2161 ttctctagta accattgaat cgcgaggttc cctatgaaac cgagagacct gttgttcgtc
     2221 gccggcgtgg cggcgctttt ctcaccgttc ttcgtttccg acgcggtgtt cgacggctac
     2281 aagatgttca acgccgatca cggcatcatc atgggcgcga tcaagttcgc tatccttgcg
     2341 acgctcggcg agatcatcgg cctgcgcatc cggaccggcg agtacgacgc cccgaacttc
     2401 ggcatcctgc cgcgcgccat cgtgtggggc tttctcggcg cggcgatcgc ctacgccttc
     2461 atcgtcttcc cgtcgggcac catcaagttc ctcgcctatc tcggcctgcc ggtgaccccc
     2521 gatacgctga aaggggcggt gaccggcatg caggtcgcga ccgcgttctc catctcggtc
     2581 ggcatgaacc tcatcttcgc gccgtggatg atgacgctcc acaagatcac cgacgagcac
     2641 atcgcccgca ccggcggcac ggtggcgggg ctgttccgcc ccatcgcgtt cggcgacatc
     2701 ctttcgaaga tgaattgggg cgtgatgtgg gggttcgtct tcaagaagac catcccgatc
     2761 ttctggatac cggcccacac ggtgaccttc ctgctaccgg aacagtaccg catcctcttc
     2821 gcggcgctcc tcggcgtcgt gctcggcgtg atactcgcta tcgccgctct gatggcgaag
     2881 aagtaggcgg aggcgcctac cggttgaccc gtccgttccg tttagcactg ccacggaaca
     2941 tcggccacgg cgcatcggcg gggccggtgc taagagaggg gatcgcgtag atagatgatg
     3001 cttcgttgtt cccgaaatag ataaccccac gatcatccat caccgggaaa aaaccggaat
     3061 atgcctctat cgcccacagc tcgttccctt cgagatcgta tgcatcgggt cccgaattgt
     3121 acagcgtgcc gtcatcgccc agtgtaagat cgccggtcca aaggtggaaa ctgtattctt
     3181 ccttcccgtc gggggcaacg accgtgacca tgtgggtatt gctataatcg catgcatata
     3241 tactgtcttt ttcgtcaatg atcataggga tacccgaagg ccccttccat ttcgtcacgc
     3301 catcgacccc tagcgcgaaa agactgacct catcgggagg atcggaaaga agtcttccgc
     3361 cgatatatac cgtatcatcg cgaccgatga ccagcgacgc gtaaatgtga tcatacttgt
     3421 cgctcgacac ccatcgctgt tctccctccg cggtcacggc gacgatacga taatagggat
     3481 acccttccgc catgcctccc acataggcca catacagagc atcgtcgctc ccgacgacga
     3541 tcaccgaaaa aacctcattc tcgagatagg agaatgacca tttcagcgca ccttcggagg
     3601 aaacggcgtg gacatagacc ccatccgaca tatagatggt cccgtcgttc gcgacggccg
     3661 acgacaaagt ccccccatac tgtttcttct caggaacgaa ccgccatttt tcgctcccat
     3721 cgacagggtc ccgcgccgag agttctccgt tcacaaggac atagatcgaa ccgtcattcc
     3781 caatgacagg aaaattgtcg tatatcccgg aggtcttcca tttgagcgca ccttccggac
     3841 ttaccgcgaa aaggtagccc cctgatgaag ggacatagag cgtaccgtca ttccctatac
     3901 acggcatctg cccctcgaac ttcgcatcta cggtaagtcg ccacagagga ccgacgcatt
     3961 tctgcccgga agaacagtag gaatcatccg cacaggtacc acaggatatc gtctgggaac
     4021 cgtaaaaggt ctccgccgtc gcatcgccgc attcgaactc gccacactcg ggcgcatctt
     4081 cgtagaggac acactggtta ttggtcgaac aatactcata ggaactgtaa caggtaccgc
     4141 aggaatgtcc ctgatcctcg ccacactcct ttttgtagca cggttcgcat tcctccccga
     4201 cgcaagtctc gacatcgcac ccggaaaatg caaaaaggag tgcaaggaga aaaaacggat
     4261 atcgccccat ttacgacacc tcttgaagga gccctattca gaaagagaac gggataccga
     4321 ccatcccctc accacggacg ggtcgtcggc atgagtttga tggtgccgac gagcgtaaga
     4381 ctcttcaggt cgtcatcgag gccgccggtg atgtagagca ggttccccga ccgggcgacc
     4441 ctgtggagcg cccgccccgg atccgactcg ctggacccgt tgaaataatc gccgaaccgg
     4501 ggagcgggag cgccgtcgtg ctgcgccggg tcccaatcgt tctcccagta catcatatcg
     4561 agatcgatga gttggtagaa gttcgtcgca ccgacgctcg tctgggtcgt atcgaagccc
     4621 tcggcgccgc cgatcaccga aaaatacccg ttgtagcctt cggtgacatc gagcgtcact
     4681 tcatggaacc aggtgctctt gggaaagatg tcgcccgacc tataggtgcg gtccgccatg
     4741 ttgaaacggt agccccgcgc atgcatctcg aacgccgtcg cgtccttggg caggtcgagc
     4801 ccgttcgccg cgacgatgat gtcgccctgc gccgccgccg agccgaagaa cagattcggc
     4861 gtgtcgtcgt tccattcctc atacacatcc ttcgagatga tgtgggtgaa cttcgcggtg
     4921 ctcgtcgcct tgcggccgcc gatcaccagc acccgcggag tgccgttgtc gagtcccgcc
     4981 ggcgccacga ccggcatcac gaaggtctcg gtcttgtcgc tgtggtattc gttcaccgcc
     5041 gcgctgatgg tcccgtcctc gccgaccgtc acctcgatgc cgtatatctt cgaaaaagcc
     5101 gcgccgtcct tcgcgccgcc gaccacgaag agcgtgccgc taccggtcgt ctcgtcggtc
     5161 tgcacgaagg cgtacccctg atagggggcg aagatgctcg gccagtcgag cgccccctcg
     5221 acccaatcca ccgtggtgtt atcggtcgtg gtcaaccgct gattcttccg gggaagtccc
     5281 accgtgtata tctccatccc ggccggcgcc ccattgaggg tgaccggcag gtcgggatag
     5341 tcggtgacgg aggtcttttc catcttcatg ctgctgacac cgccggcgat gagcacccga
     5401 ccgtccggaa gcgggaccac ggtgtggaac gcgcggaact ccttcatgtc ggctatctgg
     5461 tccaccgtcc cgctctcgac atcgagcagt tcgaccgtgt cgtcggcatc ccaggtcccg
     5521 ttgttggtca gggtgaaccc gcccgttatc aggatacgac cgtcgggcag ggtcgtcgcg
     5581 acatgcccga accgcggcgt ttgcagtttc ttgggaaaac agccgatgcc ggagaccggg
     5641 taaagactcg cgtacacatt ggtcttcttc ccgctcacaa aattgagccc ctgcaccgtt
     5701 ccgacccatt tcggcgccgg gtcgctgatc gactcgaacc cgtagagttc cagccgcgcg
     5761 ttggaaacat cgggtatgcc cgatatcgtc acatcgccgg acaattgatc tttggtgaga
     5821 gaggtcgtgt gcctcggctt gggattactc ccgtcatata tccggatggt gagcgcgccc
     5881 aatttatcct gtatgttgtc gccgacatag tcctcgcagg cggtgtcgtc ggccgcgagc
     5941 agggtcatgg cgacccgcat ctcgtcggcg gggacggcat cggccgggga ctcgtcgccg
     6001 caggagatca gaaataccgc cggcacgacg aacgccgcca gccgtccaaa gaaccgttcc
     6061 atcatcgtcc tctcctctcg atcaaacagc actctttccg ttcatggtcg cgcccatcat
     6121 atctttatgg aaggaaaatg caataaattt aaggccacga aaaaacggga aggttggtcg
     6181 cggcgtcgca tatctcgacc ttccggaccg cgccccccgc gacggtcacc accgcgtagg
     6241 tcggcgtacc gtcgacgccc ggtcgcccga gcgagccggg gttcaggtag aggatgccct
     6301 cctcttcgcg cagcaacggc tggtgggtat gcccgaaaac gacgagccgg ggacgactcc
     6361 ccgctatctc acggcggttc tccggcagga gccggtgggg cgccgtgagg ttgtgtacga
     6421 gaaagaagcg gacccccgcg atatcggcac gcaccgtgtc ggggagttcc ggcagcgcgg
     6481 tatcggtgtt gccgcgcacc gcatggaccg tgtcgaagca atggagcgcc gcaaggaccc
     6541 gatcgtcgcc gatgtcaccg gcgtggagca ccgtgtcggc ggcgatgtcg catatccggt
     6601 cgggcaggga cccgtgggtg tccgagacga ccagtatccg gcgctcgtcc ggaggatgtc
     6661 cgttcttttc tgtgttcatg gggccgagtg tatcacgggc gtcgcgacaa gtcaaacgga
     6721 atgtcgcggg atgcactcac ccgttattgt atgttgcaga tgcccggaat ggcggcgcgg
     6781 aatgtgtcgg cagggtagtt cttcttatcg accgtgaccc ccgcggcggt gaccgcgagt
     6841 tcatgaagcg ccggtttcgc ctctttgctt gcggtgcagg tgagcatcac cttcccgtcg
     6901 tccatcttcg agacgagcca ttcaagagac ccctcgacgc cgcggtcaag caccgtgaac
     6961 ccggcctgcg cggtaagttc cttcggcaat ttgtcggctt cggcgatcgc cgcggcaaga
     7021 ccgctggtct gcgcgatgag cactatccgc tggagtttca ccgcatcgct cggaacgccc
     7081 gtcgggatga gggcgagcgc atcggccgac ttacccagtt tttcgccgac gagcagaaga
     7141 ttagcgagga tgtcgcccgt cgcgagatcg agtttgtcac gcagttccca cccttcggcg
     7201 aatttatcct gcgagaccag cgcgtagaaa agcccttccc acgccgcgcc gttacgtgga
     7261 gaactcttcg ccgccgcgcg gaagtgggtc tcggcggtcg ccatatcgtt cttctcgtag
     7321 gcggccgagg cgaggaaata gtgcgctccg gcgtggcccg gctccttggc gatgacatcg
     7381 ttcatgcggg ccgccgccga ctctttgtcg tccgtttcgt aggcgatgag cccgaggttg
     7441 tagagcgatt cggccggctg gatgccgttc tggatgcaga ccgcgaagag acccgccgcg
     7501 tcgtcggcct tccccagacc ttgaagcgcc agagcacgga agaagagcgc ttcgggatcg
     7561 gcgctttttt cgaggatcgg tagcgcttcg ccatacttct cttccatcac cagcgaccgt
     7621 cccttcacga aatcggggtc ggcggtgttc tcttccagca acgcgagatt gcggtcggcc
     7681 tgtttctgct gggtcgtctt gccgccgcag gcgacgaaca ggagagcgat cgcgccgaga
     7741 acaagtatcc ttttcattgc agtcccgcca tcttctttat ctcggcgtag gagtgtttgc
     7801 cgttggtcgt ctccttcacc acgccgttct cgtcggcaaa gatggtgaac ggaagcagga
     7861 tgccggcgtt ctggagagat tcgggcgcct gaatgacctc gaaggtgact ccctgtttct
     7921 tgactatctt ccgtgcattc gcggcaagcg gatcgacgct tatcgagatg atcttgacca
     7981 accccttcgc ctcttcgtac tccttcttta tccgttcgat gtcggggaac atctcgatgc
     8041 aggggacgca ccaactcgac cagaaatcga cgatgatctt tttccccttg tggtcggaga
     8101 acttcacctc ttcaccggtg atgagcgacc gcatcacgat gccgtggaga ttcttcccct
     8161 tctggaaatc ctcgtcggtc agttcccgtt cgagcgcctt gtcgacctcg tcgagcgcct
     8221 cgggcatctt gggctgggtc acgaccggcg cgaccggctc ctcctttttc gtgcaggaaa
     8281 agagtaaaca gcacgaaagt acgaacacgg cgatcgagcg catgacgccc tcctagaaat
     8341 actttttctt gaagatgacc gggcccctcg cccgtttcac gacggtgccg atgatatgac
     8401 cgccggtgag cttcgccgcc tcggccgcgt gtttcttatc gacgacgcag acgaatccga
     8461 cgccgcagtt gaagacggtg aacatctcat gctccgatac gccgcccgct tttttaagcc
     8521 actggaagag cggcggaatg gtgaagtcgc cgccgatctc gaccgcgagc ttctccggga
     8581 cgacgcgcgg gatattgtcg taaaaaccgc cgccggtgat gtggaccatc cccttgaccg
     8641 gcaccttttt gagcagtttc ttaatgagtt tcgcgtagat gatggtgggc ttcaggaatg
     8701 cgtcggcgac cgtcgcgccg agttcgggga tgcgggtgtt gatcttcagt ttctgctggg
     8761 tgaagaggat gcggcgcacg agcgagtagc cgttggagtg gacgccgctc gacgggatgc
     8821 cgataagcac atcgcccgcc ttcaccttcg agccgtcgat gatcttgtcc ttgtccacca
     8881 cgcccaccga gaacccggcg aggtcgaatt cccccggagg atagaagtcg ggcatctcgg
     8941 ccgtttcgcc gccgacgagc gcgcagccgg cctgcgcaca gccctcggcg atacccttca
     9001 cgacgagcgc cgcggtgtcg gccgacagtt tcccggtggc gaagtagtcg aggaagaaga
     9061 gcggctcggc gccgaggacg agcacatcgt tcacgcacat cgcgacgaga tctatgccga
     9121 tggtgtcgta ccgcttcgcc atgaaggcga gtttcagttt ggtgccgacc ccgtcggtgc
     9181 cgctgacgag caccggcttt tttatccctt tcggtatcgt caccatcgcc gagaagccgc
     9241 cgatcccggc gagcacctcg gggcggtgcg tctttttcac cagttccttg atcatcggga
     9301 cgaggcggtt gccctcctcg atatcgaccc ccgctttccg gtaggcgtct ccgctcatgc
     9361 gacctctcct ctgaatgaat gatcgagccg ccccactata ccgtaacggg gatgattgtc
     9421 aattgaacga ccgggcgaaa aaaaagggcc gccccctgcg gagcggcccc gtgaaagcgg
     9481 gagcgagaga tcggatcaga gcgccttctt gaacgcgccg gccgctttga acgacacggt
     9541 cttcgaggcc ttgatctgga tcttctggcc ggtcttcggg ttgacaccct tgcgcgcctt
     9601 gaggttgcgt ttcttgaagg tgccgaagcc gctgaggacg agtttgttct cggcgttcac
     9661 ggtgtccttc acgatctgca cgaaggtctc gtacagcgcc ttgacctgat cgttggtcag
     9721 gttgatctcc ttcgccatgt tctccttgat cgccttgatg aaatcgttct tcgtcgcggc
     9781 gacgatcttc ttggccatgt tctcctccag aaaaaaagtt aaaaaaaacg aaagccggac
     9841 gggggcagta tgcgccgtcc gtatgcggaa atcaagaaaa tcgtgccggt atcgatgaag
     9901 tttttttcag tcgctatcag caaattatgg tcgttccggc ataactgttg ataaggtcta
     9961 tcgttttttt cttggccgca cggagcatat cgcgcttctg atgataaggc acaaaggccg
    10021 atttcacgcc gtttatcacg aggtcgagta tctgctgcgg cgcgagaccg aacagccggt
    10081 acgccttgag atactcgtcg gtcatggtcg tgtcggtgat gagcagattg tcggtgttga
    10141 gcgtgacgcg caggccgaga tcgaggtaga acgggaacgg gtggtcctct atccgcttca
    10201 ccgcgccggt ctgcgcgttc gacgagagac acacctcgag cgggatacgg tggtcgttga
    10261 catagttgag cagatcgccg tcctcgcgga gccgtgtccc gtggccgatg cggtgcgcgc
    10321 cgcagtagtg gagcgcctga tggatgctgt cgggcccgta ggcctcgccg gcatgtatcg
    10381 tgacattcac gttgttcgac aggatgagcg agaaggcgcc gagatgatcg cgcgccgggt
    10441 agttgagttc ggcccccgcg aggtcgaagc cgaccacccc cttgttcttg aaggcgacgg
    10501 cgagttcggc catccgcaga ctcaccgcgg gcgatatgtt gcggatgccg cagatgatga
    10561 cgccggaaag tatgccgaga tcgcgcttcg cgtcgcgcag gccgtgcagg accgactcga
    10621 cgatcgtcgt gagcggcagg cctttctgcg tgtggagcac cggcgaatag cgcacctcga
    10681 ggtaggtgac cccttccttc gcgctgtcct cggccagttc ataggcggcg cggtaaagcc
    10741 cctcttcctc ctgcaacacc ttgagcgtcg tctcgaagcc cttgaggtag tcgttgagcg
    10801 acgcgtgtac cttccccggg ccgatgagat gtttcagccc gtcgaggtcg cgcgcgccga
    10861 gatcgacctt ctgccgctcg gcgagttcga gtatcgtcag cgggcggagc gagccgtcga
    10921 ggtgcacatg aaggtcggtc ttgggcaggg agcggaagaa atcgcgatcg aacggacgcg
    10981 gcgcggcgac gctcactcga gcaccttgca ctcgaccggg tccatgacgc gcaggtcggc
    11041 gtacccgtcg aggtggagcg gctcgccgtc gcggatactg ctcgagaaga ggttcacgcg
    11101 cccctcgagc gcctcccgct cgaagcatat ctgcgctatc tcgccgtcgg tgcgggtcgt
    11161 ttcggtcagg tagagtttgt cgttgttgag gtagaacacc tccttcttgg tcatcgacga
    11221 gccgtcgagt tgcagaacga aggtggtcgc ctcgccgtcc tgcaggtggt cggtgaagaa
    11281 tacctgcagg agcgggatgg agaagccggc gctgttgttg atgttcttct tcatgaccca
    11341 gagcgcgccg atgtagacgg tctcctccag cgcctcgttg aggtaggtca gccgcccgtc
    11401 gccgaactgg agatcggcgg tcggggtgtt ctcggcgttg atgatgccgt agaactggaa
    11461 gcgggcctgc cacaccttct ccggcggtgc gtcctcgtcg gggtattcgg gatagacatc
    11521 gtcgttgtag taactggtca ggtcgttgag atagtcgtac ttgccgatgt cgtccttcgg
    11581 ctcctcgctg caggccgcgc agatcaggag aacgagcggc gccagcatga ggcacaacct
    11641 tcgtgtcacg ggaacctccg tctcgggatg cgtggtaacg ggccgattat agcacggccg
    11701 ttcgattgcg caacttttct tgtcttgcgg ggaaaaagga tgtagaaagg ttcggtggcc
    11761 gccgaaggag cggcgggacg gtttcgcgca gaaggagggg ccggtgacga atgaacgctt
    11821 tcaggaacgg ttcgccgaat tgcaaaaagc attctctcga ctcgaggagg cgctcgcggt
    11881 cccggtgacc gacgcggtca tcatggacgg cgtcatccag cggttcgagt tcaccttcga
    11941 attaacatgg aagacgctga aactcctgct cgatcacaag ggactcgcca ccgtcgccgc
    12001 gccgcgcgat gtcctgcagg gtgccttcgc ggcgggtatc gtttcggacg gcgaggcgtt
    12061 catgcagatg ttgcgcgacc gcaatctgac gacccacatc tacaaggagg acatggccgc
    12121 tcagatatac gaccgcatca agaacgccca cgccaacgcc ctgcgcacac tcatcgaaca
    12181 tcttgcggcg gagaagatcg cgtgaatttc ggacttcccg acaaggtgct tgccgagata
    12241 cgggccgtgc tggcgaagta tcctctggca aaggcggtcg tgtacggctc gcgggcgcgg
    12301 ggcgaccatc gtcccaattc ggacatcgac atagccgtct ggccgaagga aggggccacc
    12361 gaccgcatcg cgatgctcgc cgccgaactc gacgggctcg acatcatcta caaaatagat
    12421 ctcctcgaga tgcgacccga caccgatccg acgctcgccg cgaaggtggc gcgggacggt
    12481 cgggagttgt aacggagtgg ctgaaagaaa gattccgctc cggtacctta tgcgctaaag
    12541 agttgaaagg gagcaaccac cgccgctgcc actgttattt tcgtcttgat catcactaaa
    12601 agcttttgca caatcagcgg tgcaaaagaa accttgatat gttttttccg cccaatcaca
    12661 ttcctctctt aatgtgttta ttatcccgtc accacaatat gcgtttttgc acgatgctgt
    12721 gcaatcatcg ttgtcattca aattgccatc gtcgcattcc tccaagccct ccaacatacc
    12781 gttcccgcag gtggccttgg cggcatccca acagtaagtg gtgtcaaatc ccatgcaatc
    12841 gctttggcag ggcgcccaga gccagtcggc gtaatcaccg aatttgtcgc atgattgtga
    12901 gtctcggtca cagatttctg ttccttctct gatgccgtca ccacagatca cctcattgca
    12961 aacagtggtg agataccctg aacaatcgtt gttacaagga gcactcgtgc ccgacgcata
    13021 agggccaagt atgccacaat cgacactgtc gccatcgcaa gtctctcccg attccctaat
    13081 accgttgccg cagatctggt cgaggccatg accggtcaga gactgaaagg cttcgttaat
    13141 gaagtccata aaagcaagag ttggcatata gtgacctccc tcttccgggg aacaatcgcc
    13201 accataggaa gtaactccct cgataactgt cctgccgttc tgaacggtga aaaatggccc
    13261 tcccgaatca ccaaaacaac cacttgacgg gtttccgacc acaaggtctt ttccgaacgc
    13321 actgttccaa tactctagga tttctgctgt gccccatcta tgtacgccgg aatcactggc
    13381 ggtatcggaa gtcacaccat agccgacaat aactccggtg tcccctacct ctgattgggg
    13441 ttcttgtcgc aaaccatata tctccagctc ggttatgcgt cggtccaatt ttatcaagga
    13501 taggtcatct ttatagccta gcgtttccaa ataaccatcc caatcaggat gtttaataac
    13561 ttgtgtagcc tgagctatgg atggggatga tgagatttta gccccgttat aaatagtcaa
    13621 caacgacgga tcacttacag cgtccaatat caaatcatcg ccttctcggt tatagacgca
    13681 atgcccagcg gttaaaagaa cctccggatc aatcaaagtg gctgtgcaaa gccccattcc
    13741 tgctgtagtg tccattatca aagcaacgac tccttggtat tttgcgtagt cggtttcctg
    13801 tccgttgacg atggtgtcgg gccagtcgtt cccgtagaga aaaaaagcaa aagataccag
    13861 ggataacgcc gtcaaggctt ttctcataac acacccccgt gcgatccatg tccctcaaac
    13921 cccgcattct atctttcata aaaaccatgt caagcaaaag cactctctcg caccggcgag
    13981 taaagttgca acggcgcggt atctgtggta aggtcgcccc ggcaaagaaa gtaaggaccg
    14041 aggacccgga gcggaccatg cgtgtttcgg tatatcgcga ggcgaccttt tcggcggcgc
    14101 accacctgcg agaatatcgc ggcaagtgcg agaacctgca cggccacaac tggcgggtgc
    14161 ggctgtatgt cggccgcgac gaactgaccg ccgacggctt cgtgatggac ttcaaggaga
    14221 tagacgcggc gctcgcccgc atcctcgaac cgctcgacca ccacgacatc aacacggtgc
    14281 ctcccttcga tacgcagaat ccgaccgcgg agcacatcgc acattacata ttcgaacgcg
    14341 ccggggccga gatagcgcgg cgcgacccgc tctgccgcgt cgagcgggtg atggtgtggg
    14401 aatccgacag atcgtgcgcc atcgccgaga gggaccgatg agttaccgcc gcaatgtggc
    14461 gcttgtggtc gagaacgacg ccgggctcgt actcatgggc gaacgaagcg acatcgcggg
    14521 ggcatggcaa ctgccgcagg gaggcataga gaagggcgag gactggcaac aggcggcatg
    14581 gcgcgaactc gaggaggaaa cggggctcac ggccgccgcg gtgaccatcg tcgcccatgc
    14641 ggggccgttc cggtacgacc tgccccgccg gtcgaaacgg ggatttcgcg gtcaggagca
    14701 gacctatgtg ctcttccggc tcaccgatac cgcggccgct ctccggccgg gcgtcgagtt
    14761 ccgctccttc cgctggatga tgcgggaaga ggcgaccgcc gcggtgatag atttcaagcg
    14821 cccctgctat gaggcggcgt tccgcgcctt ccgggacaga ccggcatgag gaagaggacg
    14881 agcgtggcag gcctgatgag aaagaaaggg ggggcaggcc ggacattccg ggccgtcgtg
    14941 ataggtctcg cgatcttcgg gatcatcgct ctcatcggcg cgatcttcgg gtaccgctac
    15001 ctcaagtccc agataccgca catcgagacg atagccgact acaagccggc gcaggggacc
    15061 aaggtctacg cttacgacgg ccaactcatc gccgagttct acaaggagaa ccgccgcaac
    15121 atcgtcccct acgacaagat cccgtgtctc ctcgcgctcg ccttcgtcgc cggcgaggac
    15181 ggcgagttct tcaagcacaa ggggatcgat ccggtcgcca tcatgcgcgc cgcgttcaaa
    15241 tacgccttca ccggcgtcaa gcagggcgga tcgaccatca cccagcaact cgcgaaggcc
    15301 ttcgtcggca ccgagcggac ctacacccgc aaactcaagg atatgctcct cgcgatggaa
    15361 ctggagcgcg cgctcactaa agaggagata ctgtacctgt acctcaacga gatattcctc
    15421 gggagcggcg cgtacggggt cgagtcggcc gcgctcacct acttctcgaa gcatgtgtgg
    15481 gaactcgaac tcgccgagat ggcgacgctc gctgggctcc ccaaatatcc ctccgaggcg
    15541 tcgccgaaga acaatccgga aaaggcgaag aagcggcgcg actatgtgct cggtcggatg
    15601 cgcgccgacg gttacatcac gcaggaacag tacgacgcgg cggccgcgac accgatgacg
    15661 gtcatcccga tgaaggagat attcctcgac aaggccccct acttcagcga gaaggtgcgc
    15721 cgccacctgc agacggccta cggcaacgac cgcctgtacg acgaggggct cgtcgcctca
    15781 acgaccgtcg atgtccgcgc ctctcagata ctcgacgagt cgctctacac cggcctgcgg
    15841 gcgctttcgc ggcgacaggg ctacaccggc ccgctctatc atatcgacct tgaaaaggac
    15901 cgtgaccgct accttgagcg acaccgtcgt tctttcggcg acatatccga tctcaccatc
    15961 cggcgcggca cgctctacca aggggtggtg ctcgacacag ccaaggataa ggcgaccgtg
    16021 caggtcggcg cggtgaaacg accgcttcac ctcaagcaca tgcagtgggc gcggccccac
    16081 aatccgctcg gcggctgggc gaacatcgct tcgaccgatc aggtgctgaa gccgggcgat
    16141 gtcatcctcg tccgggcgac cgacgaacag ggggtcatcg aggaactcga ccaccggcgg
    16201 accgacctgc cgcttccccg gaattttttc ttcgcgctcg aacagttgcc ggtgccgcag
    16261 ggcggcgcca tcgtcaagga cccctattcc ggctacatcc gcgccgtgat gggcggctac
    16321 gatttcgagc agagcgagtt cgaccgcacc acacaggcgt gtcgccagcc cggctcggcg
    16381 atgaagccga tattctacgc cggcgcactc gagcagaagg acgccgaccg gaaattcaga
    16441 ttcaccgccg ccactatgct gctcgatgcg ccggtcgcca tcaccgacct caacttcaag
    16501 ccggagaacc atgacgagac cttcaaggga gaggtgacgc tgtgggaggc gatcgtcctg
    16561 tcgatgaacg cgccggcggt caggatgttc atggacatcg gca
//
LOCUS       DAOQUE010000005        14959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_4521_length_14959_cov_4.312131, whole genome shotgun sequence.
ACCESSION   DAOQUE010000005 DAOQUE010000000
VERSION     DAOQUE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14959
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4521_length_14959_cov_4.312131"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKH10_00305"
     CDS             <1..289
                     /locus_tag="PKH10_00305"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNT26593.1"
                     /translation="AKIAEETAELKETVASGDKDKTFHEMGDLLAAVVNLSRKLGVNP
                     EDSLRHCALRFRARFNKMEEIDPTVTDGKHSLDDLEALWQRAKKELAKKKL"
     gene            351..1577
                     /locus_tag="PKH10_00310"
     CDS             351..1577
                     /locus_tag="PKH10_00310"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT26594.1"
                     /translation="MIKYPLILAVLMLAVSGCNNGKTSADNEQVPDDGVTDDTVTDET
                     TDDALPDPTDQSDQTTDDSTVVTDDNIVETDDDPIVSDDNAVVTDDSLLLDEDATDDL
                     LTDDGFSVDEDAVTMALLCTGQTKCYDGSAEMICPAEGNAFYGQDAQYAALGKCAPRN
                     YVVSGSGGSDIVTDEVTGLIWQRTLTGSTDQWQGAIDHCADLTYGGYDDWRLPTRKEL
                     ATLPDYSGHYPAIDDTVFPETPASPFWSSSSYAYDTTYAWLVYFDDGAVDGNGKSSSG
                     HARCVRGAALPDSAFIEATVAGKVIVTDTRTGLIWTKEYNSTVTWQDALNHCEGLDHG
                     GATDWRLPNIEELETLIDDATYLPVSDFPGMPSVDFWSSSSYATNATTAWYMNFYLGY
                     TSTYYKTNSRYARCVR"
     gene            complement(1756..1944)
                     /locus_tag="PKH10_00315"
     CDS             complement(1756..1944)
                     /locus_tag="PKH10_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26595.1"
                     /translation="MKKMLAMALMVAAMSFGTADLMAGDGCGCGKGTAKSECKCGKDC
                     KCGCQEGKECTCGCGKKK"
     gene            2117..3490
                     /locus_tag="PKH10_00320"
     CDS             2117..3490
                     /locus_tag="PKH10_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT26596.1"
                     /translation="MGRKLLIADDDASFREVMSFTLTEAGYTVDAAADGLAALELFRR
                     QRHPVVITDLKMPGMDGMELVKRINALASGTLVIVITAFGEVDTAVAAMKAGAFDFIP
                     KPCDRDHLKIVVERAFEHARLAAENAVLKRAPSSEGKELIYRSVAMHTVVGVADRVAE
                     SDSTLLITGESGTGKELLARRIHRRSARASGPLVVVNCAAIPGELLESELFGHRKGAF
                     TGAVRDARGKFELAHGGTLFLDEVAELPLNLQPRLLRALQESAIDVVGGEEPVPVDVR
                     VIAATNRDLAKLVDEGKFRLDLFYRLNVIVLHIPPLRERSEDIAPLAKHFLATYGGGR
                     SFELGDEALRRMRRYRWPGNIRELENICQRVTLLTDGGVVPLELIPETVPEAEGMVVS
                     ATGRVQLPTGGISLTELEKEVIVQALERSGGNKAQAARLLRIPRHILLYRLEQHGITG
                     KGPDEKG"
     gene            3477..4574
                     /locus_tag="PKH10_00325"
     CDS             3477..4574
                     /locus_tag="PKH10_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT26597.1"
                     /translation="MRKGKELLSLRAWGLIWVPILAITTAHFTTNAEMVWVHDILRRL
                     YYIPIVLGAFGWGLHGALAASVASSLVYLPHAFQMFVHHFHHDPGGSTEKILEILLFN
                     IIALIAGVLVERERRERRRQEEIARKLSETLDEMKLLDEQLIRAGRLQALGEMTAGLA
                     HEIKNPLASIKGAADIIADAVPTDSPRRRMVDIQHAELERLTGLLERFLAFARPKDFV
                     VSTVDITTLAVDTVSFIAPQAAKKQVNIQTRLEPLTLSADREKLTQLFLNILLNAVDA
                     SPEGSTVTVTTERRRKGKSDYACITVTDRGAGIAAELRDKVFNPFFTTKPDGTGLGLS
                     IAARIADQHKGFIEVEDAPDGGAIFTVFLPL"
     gene            complement(4552..5679)
                     /locus_tag="PKH10_00330"
     CDS             complement(4552..5679)
                     /locus_tag="PKH10_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT26598.1"
                     /translation="MIPGYSLISYMSDKVKAGGGINLAQGLPGFPPPDELTAILRELA
                     TGPVHQYAPGSGDAELRALIPSLFGAGGPSADRCLITNGATEAVTLVYLYLKSRANGP
                     FSTLAFDPAYESYRHLPRIFGDRFHPFAAEGPLTEHVGEFRRAVRESGVRLVCVASPG
                     NPWGRIFSEKEWGTLLELSEELGFSVLADGVYRELYLNERPYLPVDRISPRLFYADSF
                     SKTLSITGWRVGFLAAHEEHLPALRQIHDYTALSSPSVLQKAIARYLAAHGGADYLAA
                     LRRQVKGSFDYLHAALTGLGFRIPPIGGGYFIWARLPDGWSDGLDFAERLYKKERVAV
                     VPGVHFSENGEPFVRFNIARPLDEIQEAARRVAGFIAAERP"
     gene            complement(5676..6353)
                     /locus_tag="PKH10_00335"
     CDS             complement(5676..6353)
                     /locus_tag="PKH10_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26599.1"
                     /translation="MSSLFRITLVAALILASCGPRFISRIDLTREQNWRPEQVTVNPE
                     LDTFLAEGELSLLAGEKRYKVDYQILCAEPSLWRIDVFGFFHVHGATIIMRGLASHVF
                     HDGIWEEPKPWPAMSASVFGIVLPYKVLSLMVGGRFDLSGECAETVEGKVCREYDLHY
                     LLRQGEIVEIKSEAIEIIKVDGTWRGTSAGSPEPFYLWPEHIEKKTDFDPAMFQPTQE
                     KDIFDEI"
     gene            complement(6350..8197)
                     /gene="ftsH"
                     /locus_tag="PKH10_00340"
     CDS             complement(6350..8197)
                     /gene="ftsH"
                     /locus_tag="PKH10_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNT26600.1"
                     /translation="MRFNYKSLIIWAVLLVILFVIFNLSKLTEQNAVEEIDMTTFLNR
                     VGNGEVKQVEIDGVEYKGSFVDGKQFIAIGPSGEEVMKILAEKKVIARYKKPEDNSLL
                     QAIFASWLPMIILIAFFVFFMRQLQGGSGKAFTFGKSKHRVISKDKNPITFKDVAGVD
                     ESKEELEEIVDFLRRPQSYTRMGAKIPRGVLLVGAPGTGKTLLAKAIAGEASVPFFSI
                     SGSDFVEMFVGVGASRVRDLFQQAKSSAPCIVFVDEIDAVGRQRGAGLGGGHDEREQT
                     LNQLLVEMDGFGTDAGVIVMAATNRPDVLDPALLRPGRFDRQVYVPMPDVKGREEILK
                     VHINNIRHDDAVDLARIARGTPGFSGADLANLLNEAALLAAKRGKEKVGMAEVEEAKD
                     KVLMGKERRSLVLTDDEKRVTAYHETGHAVVGYFTPGSDPIYKISIIPRGRALGVTQM
                     LPEKDRYDHTREELVAKVSVLMGGRCAEELFLGKVTTGAGNDIERASRLVRSMVMNWG
                     MSPEMGPINYGEHEEEVFLGRELTRRAHMSEYTAERIDREIHRIITEAYNAAAAILNK
                     HREKVEAMVALLMEKETIQKNDVDAIFGDPKAAEAPAEPISPRIDGSPA"
     gene            complement(8217..9488)
                     /locus_tag="PKH10_00345"
     CDS             complement(8217..9488)
                     /locus_tag="PKH10_00345"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT26601.1"
                     /translation="MVKKEIETNKGIDPAVPAEKKKSRLSSATAMIGKSATLEIAAKA
                     KAMNERGVNVISFSAGEPDFDTPKRIKEAAKKAIDLGFTKYTDSSGIKDLKTAIRQKF
                     LRENNLHYEEANIAVTPGAKYAIYALLQSIISPGDEVIIPVPYWVSYVSMVKLSRGVP
                     VFCQPTEEYGFRLTLNDIKPVITEKTRAVIINTPNNPTGAVYRVHDLEEIVNYLAERD
                     IYVISDEIYERLTYDTNAHVSAASVVSEKLRRNVVVVNGFSKAYAMTGWRIGYVAGPR
                     DVMDRAKHFLDHTTSNTNTITQYAAIEALNGPQTDVESMVSKFDRRRRFIYEEVSKMP
                     HLRCIRPDGAFYLFVNFQKYLGGTIGDHKVNSTIELANLLLDEAHIAVVPGEAFGAPG
                     YIRFSYATSLDNIAEGLDHLRHMLADASLGD"
     gene            complement(9517..11454)
                     /locus_tag="PKH10_00350"
     CDS             complement(9517..11454)
                     /locus_tag="PKH10_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNT26602.1"
                     /translation="MAENVIKLPRLFGKYYLLELINVGGMAEVYKAKMFGVEGFEKIV
                     AIKKILPEVAEDGDFISMFIDEAKIAVQLSHPNIVQILELGKIEDSFFIAMELVNGKD
                     LKTIRKRLKKVELNMAVEQSAYIISQVCDGLDYAHRKTDEKMNPLNIIHRDISPQNII
                     ISYEGNVKLIDFGIAKAKSKSTKTQAGMLKGKFSYMSPEQVAAMPLDRRSDIFSLGVV
                     LFEMLTGKRLFLGKNDVETLEKIRKAEVPPPSVFNHDVAPELDRIVLKSLARDRDERY
                     QWASEFADDLKRHIFSLQRRFDRQDVMNFMSEFFADELEEDSAKLAEYAKLKMPKQQA
                     PQIVAPTVRSDDDDDDDDDRPSPRRGSGSGAKTWLILLAAMVGAAVLGVAGWNIVKDQ
                     QNAASLIVDSNVFATVVELDKNTTQHKRCETPCKLEGILPGQHEIELRKDGYITQQET
                     LMLKTGETQTKVAKMFKVGEQTTMVEVRSEPGGARIFVNDKDSGFDTPFAVNVPAGIE
                     VTIRVEKKGFLPVQQLLGVIPVTESRKVSFIMKDSKGKDPSTVRPVHEKKEERIASKK
                     EQHAEDGLKPDRAYLTVMTTPVTQVYIDDKAIGNSPVQRYELSPGEHKIRLRNSTYNI
                     DKMVPVELKAGQHEKLVRNLF"
     gene            complement(11514..11819)
                     /locus_tag="PKH10_00355"
     CDS             complement(11514..11819)
                     /locus_tag="PKH10_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26603.1"
                     /translation="MSIFGWKQDPPFLVLDRHGLPVAGDGIDAACLLPIIETLHDALE
                     RGETDYAVWGAGADRYVAIALEEGIAIVKCGPEAPLTKVLFSREGIRERCARLQTKN"
     gene            complement(11816..12022)
                     /locus_tag="PKH10_00360"
     CDS             complement(11816..12022)
                     /locus_tag="PKH10_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26604.1"
                     /translation="MNTETMVLLALRQGNWRHAAETADKIAKDGGDPFLSAVARASRR
                     VESGDERARSLLRFWCALRWEEKA"
     gene            12156..13685
                     /gene="purH"
                     /locus_tag="PKH10_00365"
     CDS             12156..13685
                     /gene="purH"
                     /locus_tag="PKH10_00365"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNT26605.1"
                     /translation="MTTERNAIISVHDKRGIAEFAAGLAAQGITIFASGGTFALLEKE
                     RIPARRIEDHTKFPEMLGGRVKTLHPLIHGGILAKRDDPAHLAETVRHGIALFDLVVV
                     NLYPFEETVARGASPAEIVENIDIGGPSMIRSAAKNHAAVTVVTSPDDYPTVLEELKN
                     NGGTTTPETRRALATKAFALTARYDGAVAAWLSGSDIMTIQFSRKETLRYGENPHQRG
                     TLYLDTPAVAGTIATARQLGGKELSFNNYLDLEAAKNLVFDLDEPACAILKHTNPCGA
                     ARGATIAEAYERALACDPVSAFGSIIGFNRPVDVATATLLSKLFVEAIVAPAFDEAAL
                     EILRRKKNLRIIELGAPRRDIPYDHEFKKISGGLLVEDADRRVIGPDDLRIVTRRAPT
                     EEEKRMMLFGQRIVKHIKSNAVALTTANELVGVGAGQMSRIDALEIAVRKTRRDIAGL
                     ALASDAFFPFRDSIDLAASKGIRAIVQPGGSVRDEEVIAACDEHGIAMAFTGIRSFRH
                     L"
     gene            13695..>14959
                     /gene="purD"
                     /locus_tag="PKH10_00370"
     CDS             13695..>14959
                     /gene="purD"
                     /locus_tag="PKH10_00370"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNT26606.1"
                     /translation="MKILVVGGGGREHALCWKIAQSPAVTELHAFPGNAGIAEVARIP
                     KLLDDLPETICDYAERMHIDLTVVGPEKYLALGIADRFAEAGLPLFGVTAAAARLESS
                     KAFAKQIMKEAGIPTARYAAVRDLDTGLRLIRSTARYPLVLKADGLAEGKGVVIAEDP
                     ESAEKELRELMEGRFKEAGKEVIIEEFLSGEELSLLILTDGVSWRPFTFAQDHKRAGE
                     GDTGPNTGGMGSYAPVSIGTASLTTRCEERIIKPLLATLAKHGIPYRGILYIGLMIVN
                     GDPFVLEYNVRFGDPETEALLYLLEDDLVPYLRAVANGSLGSLPALSWHPGASATVVV
                     ASGGYPGSYRRGLPITGLDAVDPAVTVFHAGTARDPNGRLIAIGGRVLMVTARGDTLA
                     EATARATDNAARISWDTSFFRRDIGHRDLR"
BASE COUNT         2832 a         4688 c         4549 g         2890 t
ORIGIN      
        1 cgccaaaata gcggaagaaa cggccgaact caaggaaacg gtcgcctcgg gcgacaaaga
       61 caagaccttc cacgagatgg gcgacctgct cgcggcggtc gtcaaccttt cgcgcaaact
      121 cggcgtcaac cccgaggatt ccctgcgcca ctgcgccctc cggttccgcg cacgattcaa
      181 caagatggaa gagatcgacc cgaccgtcac cgacgggaag cattcgctcg acgatcttga
      241 ggcgctctgg cagagggcca agaaggaact ggcgaaaaaa aaattgtaag aaaatagaat
      301 ctttttcccg atatctggta tcttatatat gcaacgaacg ggagggttcc atgatcaagt
      361 acccgctgat ccttgccgtg ctgatgttgg cggtatctgg ttgcaacaac gggaaaacct
      421 cggcggataa tgaacaggta cccgacgacg gcgtgaccga cgataccgtc accgacgaga
      481 ccaccgatga tgctctgccc gacccgacag accagtcgga tcaaacgacc gacgacagta
      541 cggtggtgac cgatgacaac atcgtggaga ccgatgatga ccccatagtg agtgacgaca
      601 atgcggtggt gaccgacgac tcccttcttc ttgatgagga cgccaccgac gacctgctga
      661 ccgatgatgg tttcagcgtc gatgaggatg cggtgaccat ggcccttctt tgcaccggcc
      721 agacgaagtg ttacgacggc tccgccgaaa tgatatgccc cgccgaaggg aacgctttct
      781 acggacagga tgcccagtat gcggcactgg ggaaatgcgc gccgaggaac tatgttgtca
      841 gcggctccgg cggctccgat atcgttactg acgaggtgac cgggctgata tggcaacgga
      901 cgctgacggg aagcaccgat cagtggcagg gggccataga ccattgtgcc gacctgacct
      961 acggcggcta cgacgactgg cgtctgccga cccgcaaaga actggcgaca cttcccgact
     1021 atagcggtca ttaccccgcg atagacgaca ccgttttccc cgaaactccc gcttcgccct
     1081 tttggtcttc ctcgtcctat gcatacgata ccacctacgc atggcttgta tatttcgatg
     1141 acggcgcggt ggacggtaac ggcaagtcca gtagcggcca tgcccggtgt gtgaggggag
     1201 ccgccctgcc cgacagcgcc ttcatcgagg cgacggtagc aggcaaggtc atcgtgaccg
     1261 acaccagaac ggggcttatc tggacaaagg aatacaacag caccgtgaca tggcaagatg
     1321 cgctcaatca ctgcgaaggt ctggatcatg gcggcgccac cgattggcgt ctcccgaaca
     1381 ttgaagaact ggaaacgctg atagacgacg ccacctatct ccccgtgagc gactttccgg
     1441 gaatgccatc ggtggatttc tggtcttctt cgtcctacgc aaccaatgcc accaccgcat
     1501 ggtatatgaa tttttacctt ggctatacaa gcacctatta caagaccaat agtcgctatg
     1561 cccggtgtgt gagataggaa gtaggagccg gggcggggga taagtagcga ctttgatttt
     1621 tctgttacca ccgggcgatc cggccgccac cgccacacac cacattataa atgaggtgat
     1681 ctaaaagaaa ccggccggag tgaaccggcc ggcctttgag ggggacaaag agcggggaac
     1741 aaagaacagg cggaactact tcttcttgcc gcaaccgcag gtgcattctt tgccttcctg
     1801 gcaaccgcac ttgcaatcct tgccgcactt gcattccgac ttggcagtgc ccttaccgca
     1861 accgcagccg tcacccgcca tcagatccgc cgtgccgaaa ctcatcgccg ccaccatcaa
     1921 cgccatcgcc aacatctttt tcatcggatc tcctcctttc aagagttacg aaacacgccg
     1981 cttatagagc atgatgcgtg ccaacgacgg catgatttct gtcccctgta aaaacagtag
     2041 gttatcatca atatcggttc tgcgagtaag aaaagtgttg aaatttctcc atcactttcg
     2101 tagaatagtc ttcatgatgg gaagaaaatt actcatagcc gacgacgatg ccagtttccg
     2161 ggaggtgatg tcgttcaccc tcaccgaggc gggctacacc gtcgatgccg ccgccgacgg
     2221 gcttgcggcg ctcgaactct tccgccgtca gcgccacccg gtggtcatca ccgatctcaa
     2281 gatgccgggg atggacggga tggaactggt gaagcgcatc aatgcccttg cgtcggggac
     2341 gctggtcatc gtgataacgg ccttcggcga ggtcgatacc gccgtggccg cgatgaaggc
     2401 gggggcgttc gacttcatcc ccaagccgtg cgaccgcgac catctgaaga tcgtggtgga
     2461 acgcgccttc gagcacgcgc ggctcgccgc cgagaacgcc gttctcaaac gcgccccgtc
     2521 gagcgagggg aaagaactca tctaccgctc cgtcgcgatg catacggtgg tgggggtcgc
     2581 cgaccgcgtc gccgaaagcg actcgaccct gctcatcacc ggcgagagcg gcaccggcaa
     2641 ggaactgctc gcccgccgca tccaccgtcg ttcggcccgc gcgtcggggc cgctggtggt
     2701 ggtcaactgc gcggcgatac cgggagaact gctcgaaagc gaacttttcg gtcaccggaa
     2761 aggggccttc accggcgccg tgcgcgatgc ccgggggaag ttcgaactgg cccacggcgg
     2821 gacactcttc ctcgacgagg tggccgaact gccgctcaac ctccagccgc ggcttctgcg
     2881 agcgctgcag gagtcggcga tagatgtggt cggcggcgaa gagccggtgc cggtcgatgt
     2941 ccgcgtcatc gcggcgacca accgcgacct tgccaagctg gtcgatgaag gtaagttccg
     3001 gctcgatctt ttctaccggc tcaatgtcat cgtgctccac atcccgccgc tacgggagcg
     3061 gagcgaggat atcgcgccgc tcgcaaaaca tttcctcgcg acctacggtg gcggacggtc
     3121 gttcgaactg ggcgacgagg cgctgcggcg gatgcggcgg taccggtggc ccggcaatat
     3181 ccgcgaactg gagaacatct gccagcgggt gacgctgctg accgacggcg gggtggtgcc
     3241 gctagaactc atccccgaaa cggtgcccga ggcggagggg atggtagtga gcgcgacggg
     3301 acgcgtccaa ctgcccaccg gagggatatc gctcacggaa ctcgagaaag aggtgatcgt
     3361 gcaggcgctg gaacggagcg gcgggaacaa agcgcaggcg gcgcggctac tgcgcatccc
     3421 gcgtcatatc ctcctctacc ggctcgaaca gcacggcatc accgggaagg gacccgatga
     3481 gaaagggtaa ggaattgctt tcgctccggg cgtggggact catctgggtg ccgatactcg
     3541 ccatcactac ggcccatttc accacgaacg ccgagatggt gtgggtccac gatatcctgc
     3601 gacgactcta ctacatcccg atagtgctcg gcgccttcgg ctggggcctg cacggggcgc
     3661 tcgccgcatc ggtcgcctcg tcgctggtct acctgccaca cgcgttccag atgttcgtgc
     3721 accatttcca ccacgacccg ggcggatcga ccgagaagat actggagata ctcctgttca
     3781 acatcatcgc cctcatcgcc ggggtgctgg ttgagcgcga acggcgcgaa cggcggcggc
     3841 aggaggagat cgcgcggaaa ctatcggaaa cgctcgacga gatgaaactg ctcgacgaac
     3901 aactgatccg cgcgggtcgt ctgcaggcgc tcggcgagat gaccgcggga ctcgctcatg
     3961 aaataaagaa cccgctcgcg tcgatcaagg gagcggccga catcatcgcc gacgccgtcc
     4021 ccaccgattc gccgcgccgg aggatggtcg acatacaaca cgccgaactg gagcggttga
     4081 ccggactgct cgaacggttc ctcgccttcg cgcggccaaa ggatttcgtc gtgtcgacgg
     4141 tcgatatcac gacgctcgcg gtcgacaccg tgtcgttcat cgcgccgcag gcggccaaga
     4201 aacaggtcaa catccagaca cggctcgaac cgttgacgct cagcgccgac cgcgagaaac
     4261 tgacgcaact gttcctcaac atcctcctca acgccgtcga cgcatcgccc gaaggatcga
     4321 cggtgacggt tacgaccgaa cgccgccgca aggggaagag cgactacgct tgcatcacgg
     4381 tcaccgatcg gggagcgggg atagcggccg aactccgcga caaggtcttc aatcccttct
     4441 tcaccaccaa gcccgacggg acggggctcg gtctctcgat agcggcacgc atcgccgatc
     4501 aacacaaagg attcatcgag gtggaggacg cacccgatgg cggcgcgatc tttacggtct
     4561 ttctgccgct atgaagccgg cgacccgacg ggccgcctcc tgtatctcgt ccagcggtcg
     4621 cgcgatgttg aagcgaacga acggttctcc gttctcggag aaatggacgc cgggcacgac
     4681 cgcgacgcgt tcctttttgt aaagccgctc ggcgaagtcg agcccgtcgg accagccgtc
     4741 cggcaagcgg gcccagatga aataaccgcc gccgatgggc ggtatccgga acccgagccc
     4801 cgtcagcgcc gcatgcagat agtcgaacga tcccttcacc tgccgccgga gcgcggcaag
     4861 atagtcggca ccgccgtgcg ccgcgaggta acgcgctatc gccttctgaa gcaccgaagg
     4921 ggacgagagc gcggtgtaat cgtgtatctg ccgcaaggcg ggcaggtgct cctcgtgcgc
     4981 cgcgaggaac ccgacgcgcc accccgttat cgagagcgtc ttggaaaaac tgtcggcata
     5041 aaagaggcgc ggcgagatgc ggtcgaccgg gagatagggc cgttcattga ggtatagttc
     5101 ccggtacacc ccgtcggcga gcaccgagaa gccgagttcc tccgaaagtt cgaggagcgt
     5161 cccccactcc ttttccgaga agatgcggcc ccacgggttg cccggcgagg cgacgcagac
     5221 cagtctgacg ccgctctcgc ggacggcgcg gcggaactcg ccgacatgct ccgtgagcgg
     5281 tccctcggcg gcgaacggat ggaaccggtc gccgaagatg cgcgggaggt gccggtaact
     5341 ctcgtaggcc gggtcgaagg cgagcgtcga gaaaggaccg ttcgcgcggc ttttcaggta
     5401 gagatagacg agcgtgaccg cctcggtggc gccgttggtg ataaggcacc ggtccgctga
     5461 cgggccgcca gccccgaaaa gcgacgggat gagcgcgcgc agttcggcgt caccgctccc
     5521 cggcgcgtac tggtggaccg gccccgtcgc gagttcgcgc agtatcgcgg tcagttcatc
     5581 gggcggcggg aaaccgggaa gcccctgtgc gaggttgatg ccgccccccg ccttgacctt
     5641 atccgacata taactgatga gggaatatcc ggggatcata tctcgtcgaa tatgtccttt
     5701 tcctgtgtcg gctggaacat cgcgggatcg aagtcggtct tcttctcgat gtgctcgggc
     5761 cacaggtaga acggctcggg cgacccggcg ctcgtaccgc gccaggtgcc gtcgaccttg
     5821 atgatctcta tcgcctcgct ctttatctcg acgatctcgc cctgtctgag cagatagtgc
     5881 aggtcatatt cacgacagac cttgccctcg accgtttcgg cgcattcgcc cgacagatcg
     5941 aaccgcccgc ccaccatgag cgacaggacc ttgtacggaa ggacgatgcc gaacacgctc
     6001 gccgacatcg ccggccacgg cttcggctct tcccagatgc cgtcatggaa cacatgggag
     6061 gcaaggcccc gcatgatgat ggtagcgccg tgcacatgga agaacccgaa cacatcgatg
     6121 cgccacagcg acggctcggc gcagagtatc tggtaatcga ccttgtaccg tttctcgccc
     6181 gcaagcaggg agagttcgcc ttcggcgagg aaggtgtcga gttcggggtt caccgtcacc
     6241 tgttccggtc gccagttctg ttcgcgggtg aggtctatgc gcgagatgaa tcgggggccg
     6301 cacgaggcaa ggatcagggc cgcgacgagg gtgatgcgga agagcgaact catgcggggc
     6361 ttccgtcaat acggggggag atcggctccg ccggagcctc cgcggccttc ggatcgccga
     6421 atatggcgtc gacatcgttc ttctggatgg tctccttttc catgaggagc gcgaccatcg
     6481 cttcgacctt ttcgcggtgc ttgttgagta tcgccgcggc ggcgttgtac gcctcggtga
     6541 tgatgcggtg tatctcgcgg tctatccgct cggcggtata ttcgctcatg tgggcgcggc
     6601 gggtgagttc cctgccgagg aacacctctt cctcgtgttc gccgtaattg atcgggccca
     6661 tttcggggct catgccccag ttcatgacca tgctgcggac cagacggctc gcccgctcga
     6721 tgtcgttgcc ggcgccggtc gtcaccttgc cgaggaacag ttcttcggcg caccggccgc
     6781 ccatgagcac cgacacctta gcgacgagtt cctcgcgggt atggtcgtag cggtccttct
     6841 ccggcagcat ctgggtcacg ccgagcgcac ggcccctagg gatgatggat atcttatata
     6901 tcgggtcgct gccgggagtg aagtagccga ccaccgcgtg ccccgtttca tggtaggcgg
     6961 tgacccgttt ctcgtcatcg gtcagcacga gggaccggcg ttccttgccc atgagcacct
     7021 tgtccttggc ctcttcgacc tcggccatgc cgaccttttc cttaccgcgt ttcgcggcga
     7081 gcaacgccgc ctcgttcagc aggttggcca gatcggcgcc cgagaacccg ggggtgcccc
     7141 gcgcgatgcg ggccagatcg accgcgtcgt cgtggcggat gttgttgatg tggaccttga
     7201 gtatctcttc gcggcctttc acatcgggca tggggacata gacctgccga tcaaaccggc
     7261 cgggccgcag gagcgccgga tcgagcacat cgggccggtt ggtcgccgcc atcacgatga
     7321 cgccggcgtc ggtaccgaac ccgtccatct cgacgagcaa ctggttgagc gtctgctctc
     7381 gctcgtcgtg accgcccccg agccctgcgc cgcgctggcg accgaccgca tctatctcat
     7441 cgacgaacac tatgcaggga gcgctcgatt tcgcctgttg gaacaggtcg cgcacgcggc
     7501 tcgcgccgac gccgacgaac atttcgacga aatcactgcc gctgatggag aagaacggta
     7561 cgctcgcctc gcccgctatt gctttggcga gcagggtctt gccggtgccg ggcgcgccga
     7621 cgagcagcac cccgcgtggt atcttggccc ccatccgggt gtaggactgc gggcgacgca
     7681 ggaaatcgac gatctcctca agttcctcct tcgattcatc cacccccgcg acatccttga
     7741 aggtgatggg gttcttgtcc ttggatatga cccggtgctt cgatttgccg aaggtgaagg
     7801 ccttaccgct ccccccttgc aactggcgca tgaagaacac gaagaacgcg atgaggatga
     7861 tcatcgggag ccacgaggcg aatatcgcct gcaggagcga attatcctcc ggtttcttgt
     7921 agcgggcgat gaccttcttc tcggcgagta tcttcatcac ctcctcgccc gacgggccga
     7981 tggcgatgaa ctgcttaccg tcgacgaaac tgcccttgta ctcgacgccg tctatctcga
     8041 cctgcttcac ctcgccgttg ccgacgcggt tgaggaaggt cgtcatgtct atctcctcga
     8101 ccgcgttctg ctcggtgagt ttcgacaggt tgaatatcac gaacaggatg acgagcagga
     8161 ccgcccagat gataagactc ttgtagttga accgcatcta cgcctccggt accgggtcag
     8221 tcgcctaatg aggcgtcggc gagcatgtgc cgcagatgat cgagcccttc cgcgatattg
     8281 tcgagactgg tcgcgtagga gaaacggatg tagccggggg cgccgaacgc ctcgcccggg
     8341 accaccgcga tatgcgcctc gtcgagcagg agattggcaa gttctatcgt actgttcacc
     8401 ttgtggtcgc cgatcgtgcc gccgagatac ttctggaagt tcacgaacag atagaaggcg
     8461 ccgtcgggcc tgatgcatct caggtgcggc atcttggaga cctcttcgta gatgaaacgg
     8521 cggcggcggt cgaacttgct gaccatcgac tcgacatcgg tctgcgggcc gttcagcgcc
     8581 tcgatcgcgg cgtactgggt gatagtgttg gtgttcgagg tggtatgatc gaggaagtgc
     8641 ttcgcccggt ccatgacatc gcgcggcccg gcgacatacc ctatgcgcca accggtcatc
     8701 gcataggcct tcgagaaacc gttcaccacg acgacattgc ggcgcagttt ctcgctgacc
     8761 accgatgcgg ccgaaacatg ggcgttggtg tcgtaggtga gccgttcgta tatctcatcg
     8821 ctgatgacat agatgtcgcg ctcggcgagg tagttgacga tctcctcgag atcgtggaca
     8881 cggtagaccg cgccggtcgg attgttcggg gtattgatga tgacggctcg cgtcttttcg
     8941 gtgatgaccg gcttgatgtc gttcagcgtg agccggaacc cgtactcctc cgtgggctga
     9001 cagaacaccg gcacgccacg cgagagtttc accatgctga cgtaactcac ccagtagggc
     9061 accgggatga tcacctcgtc gcccggcgat atgatcgact gcaggagcgc gtagatggca
     9121 tacttggcgc ccggcgtcac cgcgatgttc gcctcctcgt agtgcaggtt gttctcgcgc
     9181 aggaatttct gacgtatcgc cgttttgaga tccttgatgc cggaggaatc ggtgtatttg
     9241 gtgaagccga gatctatcgc cttcttcgcc gcctccttga tccgcttcgg ggtgtcgaaa
     9301 tcgggttcgc ctgccgagaa actgatgaca ttgacgccgc gctcgttcat cgccttggcc
     9361 ttcgcggcga tctcgagcgt cgccgatttg cctatcatgg cggtggcgct actgaggcgg
     9421 ctttttttct tttccgcggg aacggcggga tcgatgccct tgttcgtttc gatctccttt
     9481 ttcaccatga tcgtgcgctc cttatcgttg ctgatgtcag aaaaggttac ggaccagttt
     9541 ctcgtgctgg ccagccttga gttcgaccgg caccatctta tcgatgttat aggtgctgtt
     9601 cctcaatcgt atcttgtgtt cgccgggcga cagttcgtat ctctggaccg gggagttgcc
     9661 gatcgccttg tcgtcgatgt agacctgcgt gaccggggtc gtcatgacgg tgaggtaggc
     9721 gcggtcgggc ttcagtccat cctcggcatg ttgctccttt ttagaggcga tccgctcctc
     9781 tttcttttcg tgaaccggtc tcaccgtgga ggggtctttg cccttcgagt ccttcatgat
     9841 gaaactgacc ttacggctct cggtcaccgg tatgacgccg agcaactgct gcaccggcag
     9901 gaaccccttc ttttcgacac ggatggtgac ctcgatgccg gccggcacat tgaccgcgaa
     9961 aggcgtatcg aacccggaat ccttgtcgtt gacgaatatt cgtgcaccgc ccggttcgga
    10021 ccggacctcg accatcgtcg tctgttcgcc gaccttgaac atcttggcga ctttcgtctg
    10081 ggtctcgccc gtcttgagca tgagggtctc ctgctgggtg atatagccgt ccttgcggag
    10141 ttctatctca tgctgacccg gcagtatccc ctccagtttg cagggggtct cgcaccgttt
    10201 gtgctgggtg gtgttcttgt cgagttccac caccgtcgcg aaaacgttgg aatcgacgat
    10261 aagcgacgcg gcgttctgtt ggtccttcac gatattccag cccgcgacgc cgagtaccgc
    10321 cgcaccgacc atggcagcga ggagtatcag ccaggtcttt gcgccgcttc ccgaaccgcg
    10381 acgcggcgac ggacggtcgt cgtcgtcatc atcgtcgtcg tctgacctga cggtcggggc
    10441 gactatctgc ggcgcctgtt gtttgggcat cttgagtttg gcgtattccg ccagtttcgc
    10501 cgaatcttcc tcgagttcgt cggcgaagaa ctcgctcatg aagttcatga catcctgacg
    10561 atcgaaccgg cgctgaagcg agaagatgtg ccgtttgagg tcgtcggcga actcgctcgc
    10621 ccactggtag cgttcgtcgc ggtcgcgcgc gagcgacttg agcacgatgc gatcgagttc
    10681 cggcgcgaca tcgtggttga acaccgacgg gggcgggacc tccgccttgc gtatcttctc
    10741 gagcgtctcg acatcgttct taccgaggaa cagccgcttg ccggtcagca tctcgaacag
    10801 cacgacgccg agcgagaaga tgtcggaccg gcgatcgagc ggcatcgcgg cgacctgttc
    10861 gggcgacatg taactgaatt tgcccttcag cataccggcc tgtgtcttgg tcgacttgga
    10921 tttcgccttg gcgatgccga agtctatcag tttcacattg ccttcgtagg atatgatgat
    10981 gttctgtggc gagatgtcgc ggtggatgat gttgagcggg ttcatcttct cgtcggtctt
    11041 gcggtgggcg taatcgagcc cgtcgcagac ctgcgagatg atataggccg actgctcgac
    11101 cgccatgttc agctcgacct tcttgagccg cttgcgtatc gtcttaagat ccttgccgtt
    11161 gaccagttcc atcgcgatga agaaactgtc ctctatcttg cccagttcga ggatctgcac
    11221 gatgttgggg tgcgacagct ggaccgctat cttcgcctcg tcgatgaaca tgctgatgaa
    11281 atcgccgtcc tcggcgacct cgggcaatat cttcttgatg gcaacgatct tctcgaaccc
    11341 ctcgacgccg aacatcttgg ccttgtacac ctcggccatg ccgccgacat tgataagttc
    11401 caatagataa tattttccga acagtctcgg gagcttgatg acgttctcgg ccattaaagg
    11461 cgatccctgt tacatttaaa tcgtcgccat actagtgttt cagaaaagac aagtcaattt
    11521 tttgtttgga ggcgtgcgca acgctcgcgg atgccctcgc gggagaacag gaccttggta
    11581 agcggcgctt cgggtccgca cttgacgatc gcgatcccct cctcgagcgc gatagcgaca
    11641 tagcggtcgg cccccgcgcc ccacacggca tagtcggtct cgccgcgctc cagtgcatcg
    11701 tggagcgttt caatgatcgg caacaggcag gccgcgtcga tgccgtcgcc ggcgaccggc
    11761 aggccgtgac ggtcgagcac aaggaacggc gggtcctgtt tccatccgaa aatactcatg
    11821 ctttctcctc ccatcgcaga gcgcaccaga accgtaggag cgagcgcgcc cgttcgtcgc
    11881 ccgactcgac ccgacgcgac gcgcgcgcca ccgccgagag gaagggatcg cccccatcct
    11941 tcgcgatctt gtcggccgtt tcggcggcgt gccgccagtt cccctgtctt agggcaagca
    12001 ggaccatcgt ctcggtgttc atcgtacccg gtcgttgacg tacccgatga ttttagggaa
    12061 agtccctgtc cttgtcaagg aatgttgctt ttcgacctgc tctctgttaa atgaaagaga
    12121 gcctgcgcgg ggccggctct ttcatggaga ggcgcatgac caccgaacgc aacgccatca
    12181 tcagcgtcca cgacaaacgg ggtatcgccg agttcgccgc gggactcgcg gcacagggca
    12241 tcaccatatt cgcctcgggc ggtaccttcg cgcttttgga aaaggaacgc atcccggcgc
    12301 gccgcatcga ggatcacacc aaattccccg agatgctcgg cggacgtgtc aaaacgctcc
    12361 acccgctcat tcacggcggc atacttgcca aacgggacga tccggcgcat ctggccgaga
    12421 cggtcaggca cggcatcgcg ctgttcgacc tcgttgtcgt caacctctac ccgttcgagg
    12481 agacggtcgc ccgcggcgcg tctcccgccg agatcgtcga gaacatagat atcggcggcc
    12541 cctcgatgat ccgctcggcg gcgaagaacc acgcggccgt gacggtggtc acctcgcccg
    12601 acgactaccc gacggtgctc gaggaactga agaacaacgg cggcacgacg accccggaga
    12661 cccgccgtgc gctcgcgacg aaggccttcg cgctgaccgc ccggtacgac ggcgcggtcg
    12721 ccgcgtggct gtcgggcagc gacatcatga cgatccaatt ctcgcgcaaa gagaccttgc
    12781 ggtacggcga gaatccacac cagcgcggta cactctacct cgacacgccg gccgtcgccg
    12841 gcaccatcgc gaccgcaagg caactgggcg gcaaggaact gtcgttcaat aactatctcg
    12901 atctcgaggc ggccaagaac ctcgtgttcg acctcgacga gccggcctgc gccatcctca
    12961 agcacaccaa cccctgcggg gcggcccgcg gtgcgaccat cgccgaggcc tacgagcgcg
    13021 cgcttgcgtg cgatccggtg tcggccttcg gctccatcat cgggttcaac cggccggtcg
    13081 atgtggcgac cgcgacgctc ctgtcgaaac tcttcgtcga ggcgatcgtc gcccccgcat
    13141 tcgacgaggc ggcgctcgag atcctgcgcc gcaaaaagaa cctgcggatc atcgaactgg
    13201 gcgcgccgcg ccgcgacatc ccgtacgacc acgagttcaa gaagatatcg ggcggtctgc
    13261 tcgtcgagga cgccgaccgc cgcgtcatcg gcccggacga cctgcgtatc gtcacccgcc
    13321 gtgcaccgac cgaggaggaa aaaaggatga tgctcttcgg tcaacgcatc gtcaagcaca
    13381 taaaatcgaa cgccgtcgcg ctcacgaccg ccaacgaact ggtcggcgtc ggcgcggggc
    13441 agatgtcgcg catcgatgcg cttgagatcg cggtccgcaa gacgcgccgc gatatcgcgg
    13501 gactcgctct cgcgagcgac gcgttcttcc cgttccgcga ctcgatagac ctcgccgcgt
    13561 ccaaagggat acgggcgatc gtccagcccg gcggatcggt ccgcgacgaa gaggtcatcg
    13621 ccgcgtgtga tgagcacggc atcgcgatgg ccttcaccgg tatccggagt ttcagacacc
    13681 tctaggaggc gaacatgaag atcctcgtcg tcggcggcgg cggccgcgaa cacgcgctgt
    13741 gctggaagat agctcagagc ccggcggtca ccgaactgca tgcgttcccc gggaacgccg
    13801 gcatcgccga ggtcgcgcgg ataccgaaac tgctcgatga tctccccgag accatctgcg
    13861 attacgcgga acggatgcac atcgacctga cggtcgtcgg ccccgagaaa tatctcgcgc
    13921 tcggtatcgc cgatcggttc gccgaggcgg ggcttccgct attcggcgtc accgccgccg
    13981 ccgcgcggct cgaatcgtcc aaggcgttcg cgaaacagat catgaaggag gcgggcatac
    14041 cgaccgcgcg ctacgccgca gtgcgggacc tcgacaccgg tctgcggctg atccgatcga
    14101 ccgcacgcta cccgctcgtt ctcaaggccg acggcctggc cgagggaaaa ggagttgtca
    14161 tcgcggaaga ccctgaaagc gccgagaagg aacttcgcga actcatggag gggcgcttca
    14221 aggaggcggg gaaagaggtc atcatcgagg agttcctgag cggcgaagag ttgtcgctcc
    14281 tcatactcac cgacggggtc tcgtggcgcc cgttcacctt cgcgcaggac cacaagcggg
    14341 ccggcgaggg agacaccggt cccaacaccg gcggcatggg ctcctacgcg ccggtgagca
    14401 tcggcaccgc ctcgctcacc acccgttgcg aggagcggat catcaagcca ctgcttgcca
    14461 cgctcgccaa acacggcatc ccctaccgcg gcatcctcta catcgggctc atgatcgtga
    14521 acggggaccc gttcgtgctc gaatacaatg tccggttcgg cgatcccgag accgaggcgc
    14581 tcctctatct tctcgaggac gacctcgtcc cctacctgcg cgccgtcgcg aacggctcgc
    14641 tcggctcact gcccgcgctc tcgtggcatc ccggcgcctc ggcgaccgtc gtggtcgcct
    14701 cgggcggcta tcccggatcg taccggcgcg gtctgcccat aacgggactc gacgctgtcg
    14761 acccagccgt cacggtgttc cacgccggca ccgcgcgtga cccgaacggc cggctgatag
    14821 ccatcggggg ccgcgtccta atggtgaccg cccgtggcga cacgctcgcc gaggcgaccg
    14881 cgcgcgccac cgacaacgcc gcccgcatct cgtgggatac ttctttcttc cgccgcgaca
    14941 tcggccaccg ggacctgcg
//
LOCUS       DAOQUE010000006        14269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_4811_length_14269_cov_4.909385, whole genome shotgun sequence.
ACCESSION   DAOQUE010000006 DAOQUE010000000
VERSION     DAOQUE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14269
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4811_length_14269_cov_4.909385"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PKH10_00375"
     CDS             <1..439
                     /locus_tag="PKH10_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNT26607.1"
                     /translation="AHGELFFSPGPSCAAVEDTLDAIPVDTVAWLVMYGITDNAPMFD
                     ELRGLTGRGALLHALLDDWATGSTPANQEAFDALAAAGADVAYYDGSMTLHHKFLVTS
                     DIVEFGSMNWTYAGCLRNDEFFFISDDAALVQPFLDHAQGLFH"
     gene            503..578
                     /locus_tag="PKH10_00380"
     tRNA            503..578
                     /locus_tag="PKH10_00380"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:536..538,aa:Ala,seq:ggc)
     gene            628..744
                     /locus_tag="PKH10_00385"
     CDS             628..744
                     /locus_tag="PKH10_00385"
                     /inference="COORDINATES: protein motif:HMM:NF028380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HNT26608.1"
                     /translation="MGKGDRKTKRGKMWRASHGKTRPSSQKIRAIKKKAEEK"
     gene            complement(786..1907)
                     /locus_tag="PKH10_00390"
     CDS             complement(786..1907)
                     /locus_tag="PKH10_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HNT26609.1"
                     /translation="MGRFFCGIFIFVLAAGCGQTKKSEPEAAPPLPSGSCPVIDAQKA
                     SELAALTLRCSDTEYPNKQCYLYEKEEDLKPTRLKNPAFYGCFDWHSAVHGHWTMAKL
                     LKKFPAMSNAAEVRAKLNEHLTPERIGKELEYFRSNPGRTFERTYGWAWLLRLAAELE
                     GWDDPDAVKWRDALRPLAAHIAEKTRDYLPRLSFPVRTGVHGNLAFSLNHMLDYARAV
                     KDTELEKVIVSRSRDLYLADTNCPVAYEPSGVDFVSPCLAEADLSRRILPQGEFVAWL
                     TKFLPDPASPDFRNLAAPPVVLDKKDYAIGHLIGLMLQRSWSFNGIASALPAGDPRKA
                     AYERLAGSHCEQGFADMAGSGYGGEHWLASFAVGATLGF"
     gene            2071..2853
                     /locus_tag="PKH10_00395"
     CDS             2071..2853
                     /locus_tag="PKH10_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNT26610.1"
                     /translation="MRFMVFEKLRYLLEDPARHLAHHQVSGHLFGIEVTLNDTSLMRS
                     IDDATYRQLARDLKEYELSVHLPFYGLNLGCFDRWIGDYSERTLREGLQLCEILGAQK
                     AVTHTTVPALLSDDSRSKWLNRFLARKESLEMHARAHGVALCWENTYETDLEIFDAMI
                     KQNHETQFCLDVGHIACFAKYPVVRIIDHLGHRITHLHLHDNDGADDRHWPLGKGTID
                     FSTVLTNLPAMKVDTAVFELEPNDFRESIPQIERIFGAQLTP"
     gene            complement(2899..4128)
                     /locus_tag="PKH10_00400"
     CDS             complement(2899..4128)
                     /locus_tag="PKH10_00400"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNT26611.1"
                     /translation="MASPSGKRRVAIVTFGCKVNLFESEQLVAALSGYTVVEPEAVAD
                     IYIVNGCTVTAAADAQVRSMARRLARSGGEVIVTGCYARTADAHRPVETGIRYLPDIA
                     AVAQHLERAVSDEFTVFRARPVIKIEDGCNGSCAYCIIPSVRGRDIRSVPPDTLEKLL
                     HSVHDRGYREVVLTGIHVGKYGHERADGARLADIVEMAHAIVGRVRLSSLEADEIDDQ
                     LLALAAAGKIVPHWHIPLQAGSDAVLRAMRRPYDTRLFAEKVRAVEVAYAEPPAIGTD
                     VIVGFPGESDADFEAAFAFISSLPFTYGHVFPFSPRPGTPAAEMHKKFGVPPAVKKEQ
                     ARRLRELFAEKKRLFQRSQTGRTMGMILETEIASDRFEGTTDTYFQLTYRGKGAIKEL
                     KRVVIRKEGKGYFAEEA"
     gene            complement(4109..4492)
                     /gene="nifU"
                     /locus_tag="PKH10_00405"
     CDS             complement(4109..4492)
                     /gene="nifU"
                     /locus_tag="PKH10_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HNT26612.1"
                     /translation="MDYTEKVMDHFMNPRNVGKIENADAIAEVGNASCGDVIKMYLKI
                     QGETIEDIKFQTFGCGAAIAVSSQTTEMVKGKTLREALDLTRDQVAEELGGLPPIKMH
                     CSNLAADALHQAVKTYMKDHGIAER"
     gene            complement(4500..5690)
                     /locus_tag="PKH10_00410"
     CDS             complement(4500..5690)
                     /locus_tag="PKH10_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNT26613.1"
                     /translation="MERIYLDYNATTPVRAEVRDAVLPFLNDLFGNPSSRHLEGRQVK
                     TKIEAARAQVATAIGAEPERIVFNAGATEGNVTILRGYQVNYKEKKGRDHIIISAVEH
                     PCIYDTAKKMAGEGYAVTFVPVDGEGNIDLDGLKKAITERTGLISVMAVNNETGVIYP
                     VKEIAKIAREKGILFHTDAAQAIGKMPFDVKDIDADFVTMSAHKFYAPKGIGALYIKK
                     GKRLDPLLTGGHQEANRRAGTENVVGMIAMGRALELATGELATDRERIGKIRDEFERR
                     ILAEVPHSRRNGGADNRLYTTSNISFRFVEGEAVQLKLDHFGIAVSTGSACSSGTLDP
                     SHVVMAMCNGDEETAHSSIRFSFGKYSKMEEIGYVVDRVKEVVAQLRSFSPLYDKFLS
                     SQGK"
     gene            complement(6127..6927)
                     /locus_tag="PKH10_00415"
                     /pseudo
     CDS             complement(6127..6927)
                     /locus_tag="PKH10_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            complement(6988..7617)
                     /locus_tag="PKH10_00420"
     CDS             complement(6988..7617)
                     /locus_tag="PKH10_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26614.1"
                     /translation="MTEPSVSQSFLATIEKNASSLVSLGEPVLDNVLSKGYLRDIPLL
                     GTLVSLGKIGDDIRGYYFVKKIWHFLKETKNISQEDRDRYIAEMELDEKKFDKACEQL
                     LLIIEKLDAMEKAQIIGRLFKARVEKRITEDQFLVASQITDRMMHGDLVLLQQYEKIP
                     DQKRQHLLGLRLLDLDKLRVRTAVAGSSVRQMFSLSEYGKILLAETKGL"
     gene            complement(7702..8190)
                     /locus_tag="PKH10_00425"
     CDS             complement(7702..8190)
                     /locus_tag="PKH10_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26615.1"
                     /translation="MDKEALVKAFHVDNEALVKGLHATIESLKKKGRDFFLVMLVPFE
                     PTSNDLRLALLISASWLDNLSPKEALDEIIQEFLNVHANKGPETDSFFKKVGRITIIK
                     STDPFVNALTSAISVSDRATAYIGEGNINGVLIKQAIILAARNPRQKKSDKKDAIPRK
                     LK"
     gene            complement(8169..8600)
                     /locus_tag="PKH10_00430"
     CDS             complement(8169..8600)
                     /locus_tag="PKH10_00430"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNT26616.1"
                     /translation="MPTTQSDLRKLARLRLREAQALFDKKLYDGCEYLAGYVIELALK
                     ARICKTLNTNDYPDTGERSRVFKTHNLDDLLFLSGLQRRFDEDKRTNPALFTNWSLIT
                     PWSEQYRYNPVGSSNKVHAKQVLEALKDQNNGVFSWIKKRW"
     gene            complement(8677..11544)
                     /locus_tag="PKH10_00435"
     CDS             complement(8677..11544)
                     /locus_tag="PKH10_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HNT26617.1"
                     /translation="MSAPQVITDLVERFKEQADFYKSNQYKEASLRKEFLDPFFAALG
                     WDMENNAGASEAYKAVCVEYPLKIQGTTKSVDYCFRIGGTPKFFVEAKRPSVNINEDM
                     DAAYQLRRYAWNAHIPIAILTDFEEFSVYDMHIKPLRGDKANKGRVYHKRFSDYEAEW
                     DTIYSRFSSNAVFKGLFDQYAEKSKGKRGTVEVDEDFLLTIEKWRNDLAHNIALRNAL
                     TQRELNFSVQRIIDRIIFLRICEDRGIEDYTRLHSLINGNDIYTRLGNIFREADARYN
                     SGLFHFKAERNIAEPPDELTLNLNIDDKLLRDILKSLYYPESDYEFGLLPADVLGHVY
                     EQFLGKVIRLTDGGRRAVVEEKPEVRKAKGVYYTPTYIVEHIVKETVGKLTEGKTPAE
                     IAKLRILDPACGSGSFLIGAYQYLLDRHLAWYLENGPEKEAKKRSPALVRTSGGWKLT
                     IAERKRILLNNIYGVDIDSQAVEVTKLSLLLKVLEGETEQSVQTMMNLFRERALPDLG
                     SNIKCGNSLIGPDFYAQAALPDMNDEERLRVNVFDWQAEFPAIFKAGGFDAVIGNPPY
                     RSLLLGKKQDSEDSDLIVYYRTKFPSAAVYKMNLFGLFMQRAVSLLREKGKFSFIIPN
                     LFFNSHYFRGLRGFMMESGSFSSVFDLRYKVFDDAETGGSGIFVFDKTLAAPYLEILT
                     ADVQNQFTAPLHNQVSAIALRSDPNFNLLASSGVTVVEDRIRSLPCITLGDACVIYQG
                     IITGDNSTFLSEKPRGSGWEKILKGRDINRYSKEFGGVYVLYNPEKLWSNTNPKMFKV
                     QEKIISRQTSDHLVATYDDEGFFSLDSTHVIHLKKPLFSLKYLLGIYNSRLLNFLYSE
                     KVKENGRAFAQVKVVNLKPLPIRLLDLSKPLDRERHNRMVMLAEKMLALHKQAAAART
                     PQEKTAIDRQISATDAQIDRLVYDLYGLTEEEIKIVEGSSK"
     gene            11685..12209
                     /locus_tag="PKH10_00440"
     CDS             11685..12209
                     /locus_tag="PKH10_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNT26618.1"
                     /translation="MPAQIEALTGDITKIAVDAVVNAANRTLMGGGGVDGAIHRAGGS
                     AILEACEAIRREQYPDGLPIGEAVATTAGELPARWVIHTVGPVWHGGKKNEDGLLLNA
                     YTNSYRVATELGAKSIAFPAISTGVYGFPKERAAGIVGRFIRETALSGPLERVLFVFF
                     SAADRQVFRNHGSW"
     gene            complement(12228..13436)
                     /locus_tag="PKH10_00445"
     CDS             complement(12228..13436)
                     /locus_tag="PKH10_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26619.1"
                     /translation="MREKCMIAVTMFLFAACDTGGGKPIGSEGGPCYDTGTCDGTLTC
                     VSEVCVDLSELVDGDTLAADSAPDDQPDEEPTDDQTDLSDPSDESVDTDTLTPDQSDA
                     SDDEVSDEDAVVAECVDGATESLDCEGSGKQIRECTGGQWGEFGDCIDITQEVIDIAD
                     VSECADYYWEDRGDAPPAYTRGMALVFAGAYCHRDRSDVALVAAAKTADTEHDALAWY
                     DEIFSTLGMDNSADGADTLRHAYTLLLGLGMRESSGEHCCGRDQSADNVTADTAEAGL
                     FQTSYNSHIFSDELDTLYADWKGSYEFANHRCLLEVFSEDVYCSDADWENYGTGEGVI
                     FQDLEKRCPIFATEYAAVMLRVSGGSLGHYGPLRRHDAEVRPECDAMFADVQSLIDAE
                     PFFCQFLQNY"
     gene            13595..>14269
                     /locus_tag="PKH10_00450"
     CDS             13595..>14269
                     /locus_tag="PKH10_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26620.1"
                     /translation="MKRSFILLLVPLLSLMIAGTGCDQKKGSGTGEKVQTKVAAAEGG
                     TVESSSGEVKMEIPPGALYEDAYISITLVNKEGYPDSGNVASKVVRCEPSGLKFKEPV
                     TLTIVADASITVPEGHRLTTSIFRNEQWENRHSGDPIMAGDPIMAGDPIMAGDPIMAG
                     TGYTSQVGHFTDFALYLQEVTEGSDDDTLLEDVDQTTDTVEAKPDTDAATDPCAGVTC
                     SGHGTCS"
BASE COUNT         2898 a         4166 c         3921 g         3284 t
ORIGIN      
        1 cgcccacggg gaactgttct tctcgcccgg tccttcctgc gcggcggtcg aggacacgct
       61 cgacgcgata ccggtcgata ccgtcgcgtg gctcgtcatg tacggcatca ccgacaatgc
      121 gccgatgttc gacgaactgc gcgggctcac cgggcgcgga gcgctcctcc acgccctgct
      181 cgacgactgg gcgaccggga gtacgccggc caatcaggag gcgttcgacg ctctcgccgc
      241 ggcgggggcc gatgtcgcct attacgacgg ctcgatgacg ctgcaccaca agttcctcgt
      301 gacctccgat atcgtggagt tcggctcgat gaactggacc tacgccggct gcctccggaa
      361 cgacgagttc ttcttcatca gcgacgatgc ggcgctcgtt cagccattcc tcgatcacgc
      421 gcagggactc ttccactgac cgcgatcgga cccgcgaaat tatgttgaca acagggccgt
      481 tttcactata aacgcctcgc gtggggctgt ggcgcaattg ggagcgcact tgaatggcat
      541 tcaaggggtc gacggttcga acccgttcag ctccaccact ttttttatgt gtcgttcgtt
      601 cgtatagcag caccgctgga ggaaacgatg ggaaaaggtg accggaagac caaacgcggc
      661 aagatgtggc gcgcctcgca cggcaagacg cggccctcct ctcagaagat ccgcgccata
      721 aagaaaaaag cggaagagaa gtagttcccc ggccggcccg acgggccatc gcgaaagatg
      781 atcccttaaa atcccagtgt ggcgccgacc gcgaaactgg ccagccagtg ttctccgccg
      841 taacccgatc cggccatgtc ggcgaagccc tgttcgcagt gcgagccggc gagccgttcg
      901 taggccgcct tgcgcgggtc gcccgccggg agcgcggaag cgatgccgtt gaaactccac
      961 gaccgctgga gcatgagccc gatgaggtga ccgatggcgt aatccttttt atccaatacc
     1021 accggcggcg cggcgaggtt cctgaaatcc ggtgaagccg ggtcagggag gaacttcgtc
     1081 aaccacgcca cgaactctcc ctgcggcagg atgcgtcgcg agagatcggc ctcggcgagg
     1141 cagggcgaga caaaatcgac gcccgacggc tcgtaggcga ccgggcagtt ggtgtcggcg
     1201 agatacaggt cgcgcgaccg cgacacgatg accttctcga gttcagtatc tttgaccgcc
     1261 cgggcgtagt cgagcatatg gttcagcgaa aaggccagat tgccgtggac gccggtacgc
     1321 accgggaacg agaggcgcgg cagatagtca cgcgtctttt cggcgatgtg cgcggcaagc
     1381 ggccggagcg cgtcacgcca cttcacggcg tcggggtcgt cccatccttc gagttcggcg
     1441 gcgagccgca gtaaccacgc ccaaccgtag gtccgctcga aggtcctgcc ggggttcgac
     1501 cggaaatatt cgagctcctt ccctatccgc tccggggtca ggtgctcgtt cagtttcgcc
     1561 cgcacctctg cggcgttgga catcgcgggg aactttttca acagtttcgc catcgtccag
     1621 tggccatgca ccgccgaatg ccagtcgaaa cagccgtaaa aggcgggatt tttgagccgc
     1681 gtcggcttca gatcctcttc cttttcataa agatagcact gcttgttcgg gtactcggtg
     1741 tcgctgcacc tcagcgtgag cgccgcgagt tccgacgctt tctgcgcatc gatgacggga
     1801 caggaaccgg aggggagcgg tggcgccgcc tcgggttcac tctttttcgt ctgaccacat
     1861 ccggccgcta acacaaaaat aaaaatgccg cagaagaacc gtcccatcgc tctcctccct
     1921 tttcataccg tcgttgtccg ttttgcatca tcgtcaaaaa gacgagcgat atcaagttat
     1981 tttgatcttc tttatcaaga cgctccgtct ctgttgacaa aatcgttgcg ccccgctata
     2041 gtcgccccgc tttttgaaac ggagtccccg atgcgcttca tggtgttcga aaaactgcgt
     2101 tatctccttg aggatccggc ccgtcatctg gcccatcatc aggtgagcgg ccacctcttc
     2161 ggtatcgagg tgacgctcaa cgacacctcg ctcatgcgct cgatagacga cgcgacctac
     2221 cggcaactcg cccgcgatct caaggaatac gaactctcgg tccatctccc cttctacggg
     2281 ctcaatctcg gctgtttcga ccgctggatc ggcgattatt cggagcgcac cctgcgcgag
     2341 gggctccaac tctgtgagat actcggcgcg cagaaggcgg tgacccatac gacggtgccg
     2401 gcgctccttt ccgacgattc ccgctccaag tggctgaacc ggttcctcgc acggaaggaa
     2461 tcgctcgaga tgcacgcgcg ggcccacggc gtcgcgctct gctgggagaa cacctacgag
     2521 accgatctgg aaatcttcga cgcgatgatc aaacagaacc acgaaacgca gttctgtctt
     2581 gatgtcggcc acatcgcctg cttcgcgaaa tatcctgtcg tccgcatcat cgaccatctc
     2641 ggccaccgga taacccatct ccacctgcac gacaacgacg gcgccgacga ccgccactgg
     2701 cccctcggta aggggacgat cgacttttcc accgtcctca ccaatctgcc ggcgatgaag
     2761 gtggacaccg cggtcttcga actcgaaccg aacgatttcc gcgagagcat cccccagata
     2821 gagcggatct ttggagcgca actcaccccc tgacccctct cttcgcaaga aaggggaggc
     2881 ggtacgccgg ggtgagttct acgcctcttc cgcaaaatat ccttttcctt ccttcctgat
     2941 gaccacccgc ttgagctctt taatcgcccc tttcccgcga taggtgagtt gaaagtaggt
     3001 gtcggtcgtc ccttcgaacc ggtcggaagc gatctccgtt tccagtatca tccccatcgt
     3061 gcggcccgtc tgcgaccgct ggaacaatcg cttcttctcg gcgaacagtt cccgcaagcg
     3121 gcgggcctgc tcctttttca ccgcgggcgg cacgccgaac tttttgtgca tctcggccgc
     3181 cggtgtgccg gggcgcggcg agaagggaaa gacatggccg taggtgaagg gaagcgacga
     3241 aatgaacgcg aacgccgcct cgaagtcggc gtcgctctcg ccggggaagc cgacgatgac
     3301 gtcggtgccg atggcgggcg gctcggcgta agccacctcg acggcgcgca ccttttcggc
     3361 gaaaagtcgg gtatcgtagg ggcgacgcat cgcccggagc accgcgtcgc tccccgcctg
     3421 cagcgggatg tgccagtgcg gcacgatctt cccggcggcg gcaagcgcga ggagctggtc
     3481 gtctatctcg tcggcctcga gcgatgagag ccgtacccgg ccgacgatgg cgtgggccat
     3541 ctcgacgatg tcggcaagcc gcgccccgtc agcccgttcg tgaccgtact tcccgacatg
     3601 gatgccggtc agcaccacct cgcggtaacc gcgatcatga acgctgtgca gaagtttttc
     3661 gagtgtatcg ggcggcacgc tgcggatatc gcgcccgcgg accgagggga tgatgcagta
     3721 ggcgcacgaa ccgttgcacc cgtcctctat cttgatgacg ggacgagcgc ggaacaccgt
     3781 gaattcgtcg ctcaccgccc gctcgaggtg ttgcgccacc gccgcgatgt cggggagata
     3841 gcgtatcccc gtctcgaccg gccggtgcgc atcggcggtg cgggcgtaac agccggtgac
     3901 gatcacttcg ccgccgctcc gggccagccg ccgcgccatc gaccggacct gcgcgtcggc
     3961 cgccgcggtg acggtacagc cgttgacgat gtagatatcg gccaccgcct cgggctcgac
     4021 gacggtatag cccgacaggg cggcgacgag ttgttcgctt tcgaaaagat ttaccttaca
     4081 gccgaaggtg acgatggcga cgcgacgctt accgctcggc gatgccatgg tccttcatat
     4141 aggtcttcac cgcctgatgg agcgcgtcgg cggcgaggtt ggagcagtgc atcttgatgg
     4201 ggggcaggcc gcccaactcc tcggccacct gatcgcgggt gaggtccagc gcctccctga
     4261 gcgtctttcc cttgaccatc tcggtggtct gggaggagac cgcgatggcg gcgccgcatc
     4321 cgaaggtctg gaacttgatg tcctcgatcg tctcgccctg tatcttgaga tacatcttta
     4381 tcacatcgcc gcacgaggcg ttccccacct cggcgatggc gtcggcgttc tctatcttcc
     4441 ccacattgcg cgggttcatg aagtggtcca tgaccttttc ggtgtaatcc atgacggcct
     4501 cactttcctt gtgaactcaa aaacttatcg tacagcggag aaaaactacg gagttgcgcg
     4561 acgacctctt tcacgcggtc gacgacatac ccgatctcct ccatcttcga atactttccg
     4621 aaactgaagc ggatgctcga atgggccgtt tcctcgtcgc cgttgcacat cgccatcacc
     4681 acatggctcg ggtcgagcgt ccccgacgaa caggccgacc cggtcgaaac ggcgatgccg
     4741 aaatggtcga gtttcaactg caccgcctca ccctcgacga accggaaact gatattgctc
     4801 gtggtataga gccggttatc cgcgccgccg ttacgccgcg agtgcggcac ctcggcaagg
     4861 atgcgccgct cgaattcgtc gcgtatcttc ccgatccgtt cgcggtcggt cgccagttcg
     4921 cccgtcgcga gttccagcgc cctgcccatc gctatcatac cgacgacatt ctcggtcccg
     4981 gcgcggcggt tcgcttcctg atggccgccg gtgaggagcg ggtcgagccg tttccctttc
     5041 ttgatataga gcgcgccgat acctttgggg gcatagaact tgtgggccga catggtcacg
     5101 aagtcggcgt cgatatcctt cacatcgaac ggcatcttgc cgatcgcctg cgccgcgtcg
     5161 gtgtggaaga ggatcccctt ttcgcgggct atcttcgcta tctccttcac cgggtagatg
     5221 acgccggtct cgttgttgac cgccatcacc gatatgagtc cggtccgctc cgttatcgcc
     5281 tttttcaacc cgtcgagatc gatattcccc tcgccgtcca ccggcacgaa ggtgaccgcg
     5341 tacccctcac ccgccatctt cttggcggtg tcgtagatgc aggggtgctc gacggcgctg
     5401 atgatgatat ggtcgcgtcc tttcttttcc ttatagttca cctgatagcc gcgcaggatc
     5461 gtcacattcc cttcggtcgc cccggcgttg aacacgatcc gctccggctc ggcgccgatg
     5521 gcggtcgcca cctgagcgcg cgccgcctcg atcttcgtct tgacctgacg gccttcgaga
     5581 tggcgggaag aggggttgcc gaaaaggtcg ttcagaaagg gaagcaccgc gtcgcgcact
     5641 tcagcgcgga ccggcgtcgt ggcgttgtag tcgagatata tccgttccat cgggaactcc
     5701 gtcaaccgtc gtcgtttggt actataatac ggtcgggagt tttgtcaacc taattctttt
     5761 aaagttgggc aaaatctttt taggcgaccc taactgtgag aaaaaccgag ggctgttcgg
     5821 ctttcgatca ccagttcagt cgcggagggt aaacacatca cggtcggaca gggatcactc
     5881 ttcttcgatg acccgattca cgggatcgcc gcacgaagcg cacgaaccgt gcagcacaag
     5941 caaccccttt tccatctcgg cgtgttccaa tctgatcgtc accgtccccc tgcatcgacc
     6001 gcaccagaca ttattcagaa cccgggcacg gaacgactcc ggtatcgctt cccaccgttt
     6061 tttcgccgcc ggagtgaatt gcgggaacgg ctctacggac gatcccagtt tcttttcggt
     6121 cgccgtttta tccagatagt tctccgtgct gacgaccttc tgcccggtct ccttttccag
     6181 tttgcgcctc gcttcgcccg cgacattgcc gccgcgtttc gacacttttt tgttctcgac
     6241 gaagccctgc ggatgctcct tcttggtgat ggcggtcgtc gccgcctctc ccagcatcga
     6301 gaagatgagt tcgaggtcgg tcatgtggtc gcgcaggttc tcgcgctgta gccctttcag
     6361 gtgtttgtgt tcgctcggtg tgacgccgaa ggcggtcagt atctcgtact ccttgctttc
     6421 cttgacaccg tgttttttcc actcgtcggt caactcctcg cggatggcga tgccgcgcat
     6481 gcgccgttct atccactctt cgggatagcc cttcgccttg tacagttcgc gcatccgttg
     6541 cgccgctttt tcgggatcct ctatctcctg cacccgttcg tagccgacct tagccagcca
     6601 ccgcttgaag ggctcggcct tcggcgacgg gatggcctgt atgatgcgga agagtccttc
     6661 ggtgttggcg cagtcggtag cgcgttgctt gccgtccggc gcttccaact tcaacccgtg
     6721 acaaaacgtc acgacttcac ttccttcttc ctgcaatctt tgtttcagct tgcgccagta
     6781 ggccccggca tcggcgctgt cggtcagggc ggcgaccaca tcgaccaccg agaagaacca
     6841 ctcctcgtta tgccagatgc gccgtatctt gcggttctcg aaggcgacga gtttgctctg
     6901 cggggtgctc gtcttgatct cgttcatgat tgctcctctc gattatgctc ttttcgttac
     6961 atcacggtcg ggcattgacc aacattccta taatccttta gtttcggcaa gaaggatttt
     7021 cccgtattct gacaaggaaa acatctgtct taccgaagac cccgctacgg ctgtgcgcac
     7081 acgcaactta tcaaggtcta aaagcctgag tccaagaagg tgctgccttt tctgatctgg
     7141 aattttctcg tattgttgca acaatacaag gtcgccgtgc atcattcgat ctgtgatttg
     7201 tgatgcgacg agaaactgat cttccgttat gcgtttttct actctggcct tgaaaagccg
     7261 accgattatc tgggctttct ccatcgcgtc gagtttttcg atgatgagca gaagttgctc
     7321 acatgctttg tcgaatttct tttcgtccaa ctccatttca gcaatgtagc gatctctgtc
     7381 ttcttgtgag atattcttcg tttctttgag aaaatgccat atctttttaa cgaagtaata
     7441 tccgcggatg tcgtcgccta tcttgccaag ggaaacaagg gtgcctaaga gagggatgtc
     7501 ccgaagatat cctttcgaga gcacgttatc aagtacgggt tcgcccaagg agaccaacga
     7561 ggacgcattt ttttcaatgg ttgcaagaaa agattgagag acactaggct cggtcatgtt
     7621 gtcctctcca ccgtctcgat ctcttcttcc ctcagttcgc ggacggcact tacctcacgg
     7681 tcgggcatag taaaacaccc atcatttcaa tttgcgagga atggcatcct ttttgtctga
     7741 tttcttctgt ctaggattcc ttgcggctaa aatgatagct tgttttatta acacaccgtt
     7801 gatgtttccc tctccgatgt atgcggtggc cctgtctgaa acagatatag cagaggttag
     7861 tgcgtttaca aaggggtctg tgcttttaat tatagtaatt ctccccactt ttttgaaaaa
     7921 agaatctgtt tccggtccct tattggcatg cacgttgagg aattcttgaa ttatttcatc
     7981 caaggcttct ttgggactta agttgtccaa ccaactggcg cttattaaaa gagctaaacg
     8041 caagtcattt gaagtcggct caaaaggcac aagcattact aaaaagaaat cccttccttt
     8101 tttcttcaaa ctttcgattg tggcgtgaag gccttttacc agcgcttcgt tatctacatg
     8161 aaaagctttt accaacgctt ctttatccat gagaaaactc cgttgttttg atccttgagt
     8221 gcctcaagca cctgtttggc atgaaccttg ttgctactac cgacgggatt atatctgtat
     8281 tgttcagacc atggagtaat taaagaccaa tttgtaaaaa gggccggatt tgtcctttta
     8341 tcctcatcaa accgtctctg aagaccggaa agaaaaagaa gatcgtcgag gttatgggtt
     8401 ttgaacactc tggatctctc accagtgtca ggataatcat tagtgttcaa tgtcttgcaa
     8461 atgcgagcct tcaatgccag ctctataaca tatccggcca agtattcgca accatcgtat
     8521 agtttcttat caaaaagagc ttgcgcctct cgtagccgga ggcgagcaag ttttcttaag
     8581 tctgattggg tcgtcggcat ccttcttctt ccaaaacata ggaaatggta tcatcgagaa
     8641 ggcaaaaagt caaaagggtc aacgcattgt ccggcattat ttagaacttc cctccactat
     8701 cttaatctcc tcttccgtca ggccgtagag gtcgtagacg aggcggtcga tctgggcatc
     8761 ggtggcggat atttggcggt cgatggcggt tttctcctgc ggggtgcggg cggcggcggc
     8821 ctgcttatga agcgccaaca tcttttctgc aagcattacc atgcgattgt gccgctcacg
     8881 gtcaagcggt tttgataaat caagaagtct aatgggcagc ggcttcagat tcacaacttt
     8941 tacttgggca aatgctcggc cgttttcctt caccttttct gaataaagga aattaagcag
     9001 gcgcgaatta taaataccca aaagatactt gagtgagaaa agaggttttt taaggtgaat
     9061 aacatgtgtg ctgtctaggg aaaagaatcc ttcatcatcg taggtcgcga ccaaatgatc
     9121 gctagtttgt ctgcttatta tcttctcttg caccttaaac attttcgggt tcgtgttgct
     9181 ccataatttt tcaggattgt acaggacata cactccaccg aactcttttg agtaacggtt
     9241 aatatctcgc cctttcaata ttttctccca tccgctccct cttggtttct ccgaaagaaa
     9301 tgtgctgtta tcgcccgtga ttatgccttg ataaataacg caggcgtcgc cgagtgttat
     9361 acacggaaga gaccgtattc gatcttccac aaccgtcacg cctgacgaag ccaacaagtt
     9421 aaaattcgga tctgaccgca atgctatagc tgatacttga ttatgaagag gtgcggtgaa
     9481 ctggttctgg acatcagcgg ttaaaatttc caagtaagga gcagctagtg ttttgtcgaa
     9541 aacaaaaatg ccacttcctc ctgtttctgc gtcatcaaag actttgtatc tcaaatcaaa
     9601 gactgagctg aaacttcctg attccatcat aaaaccacga aggccacgaa aatagtgaga
     9661 attaaagaag agattcggga tgataaatga gaatttgcct ttttctcgga gtaaagatac
     9721 ggcgcgttgc atgaacaacc cgaacaaatt cattttatag acggcagcgc ttgggaattt
     9781 tgtccgataa tatacgatta agtcgctgtc ctccgaatcc tgctttttcc ctagcaataa
     9841 actacggtac ggcggattgc cgataaccgc atcgaagcca cccgccttga atatcgccgg
     9901 gaactccgcc tgccagtcga acacattgac gcgaagccgc tcctcatcgt tcatgtcggg
     9961 caacgccgcc tgcgcgtaaa agtccggccc gatcagcgaa ttcccgcact tgatattgga
    10021 accgagatcg ggaagcgccc gctcccggaa cagattcatc atcgtctgaa cactttgctc
    10081 cgtctcccct tcgagcacct tgagcagcag cgacaacttc gtcacctcca ccgcctgcga
    10141 gtcgatatcg actccataga tgttgttgag gagtatccgc ttcctctcag cgatcgtcag
    10201 cttccacccg ccggaggttc gcacgagtgc agggcttctc tttttcgctt ctttctccgg
    10261 gccgttctcc agataccacg ccagatgccg gtcgagcaga tattgatacg cgccgatcag
    10321 gaacgagccg gagccgcaag ccgggtcgag tattcgcagt ttggcgatct cggcgggagt
    10381 tttcccttcg gtcaacttcc cgaccgtctc tttaacgatg tgttccacta tataagtagg
    10441 cgtgtagtag acgccttttg ctttgcgcac ctctggtttt tcctcaacca cggcgcggcg
    10501 gccaccgtcg gtaaggcgga tcactttacc aaggaattgt tcgtagacat ggcccaaaac
    10561 atcggcgggg agaaggccaa attcatagtc gctttcggga taataaaggc tcttcagtat
    10621 atcgcgaagc agtttgtcgt cgatgtttag attcagcgtc agttcgtccg gtggttcggc
    10681 gatattgcgt tcggccttga agtggaagag tcccgaattg taacgcgcgt cggcctcgcg
    10741 gaaaatattg ccgagccgtg tgtagatgtc gtttccattt atgagagaat ggagccgtgt
    10801 gtaatcttca ataccgcgat cctcacagat gcgcagaaag atgatccgat cgatgatgcg
    10861 ctgtacggag aagtttagtt cccgttgggt aagcgcattg cgaagcgcga tgttgtgggc
    10921 aagatcattc ctccattttt caatcgtaag gagaaagtcc tcatcaacct caacggtacc
    10981 gcgtttgccc ttgctttttt ccgcatactg atcaaacaaa cccttaaata cggcgttgct
    11041 tgaaaacctt gaatagatgg tatcccattc ggcttcgtag tcgctaaaac gcttgtgata
    11101 aacgcggccc ttgtttgctt tgtctccacg caacggctta atatgcatat cgtacacgga
    11161 aaattcttca aagtcggtaa gaatcgctat cggaatatgc gcgttccatg cgtatcgcct
    11221 taattgatat gccgcatcca tgtcttcatt tatgttcacg gacggtcgct tggcctccac
    11281 aaagaatttt ggcgtaccac ctatgcggaa acaatagtca accgacttgg tggtaccctg
    11341 tatttttagg ggatactcta cgcacaccgc cttatatgct tccgaagcac cggcattatt
    11401 ttccatatcc catccaagcg cggcaaagaa tgggtcgaga aattcttttc ggagcgaggc
    11461 ttctttatat tggttcgact tataaaaatc ggcctgttcc ttgaaccgtt caaccaaatc
    11521 agtgattact tgcggtgcac tcatggtttt ccccgtggaa agttcggccg attgtagcga
    11581 atggccggaa gaaagacaag aaaagagggc aaaggaaagg caaggacagg ttgacaggac
    11641 ccgttatatc gcgtaccgtc cgccatactt tccggagggg accgatgccc gcacagatag
    11701 aggccttgac cggcgacatc accaagatcg ccgtggacgc cgtcgtcaac gccgcgaatc
    11761 gcaccctcat ggggggcggc ggcgtggacg gcgcgataca ccgcgccgga ggatccgcga
    11821 tacttgaggc atgcgaggcg atacggcgcg aacagtatcc cgacggcctg ccgatcggag
    11881 aggcggtcgc gacgaccgcc ggcgaacttc cggcccgttg ggtcatccac accgtcggtc
    11941 ccgtctggca cggcggtaaa aagaacgagg acggcctgct cctcaacgcc tacaccaaca
    12001 gttaccgggt ggcaacggaa ctgggagcga agagcatcgc cttccccgcc atctcgaccg
    12061 gcgtgtacgg ctttcctaaa gagcgcgccg ccggcatcgt cggccgtttc atccgcgaga
    12121 cggcgctgtc cggaccgctt gaacgggtgc tgttcgtatt cttctcggcg gccgaccggc
    12181 aggtgttccg gaaccatggc tcttggtgag aggagcgagg tgttttctca gtaattctga
    12241 aggaactggc agaagaaggg ttcggcgtcg ataagggact gaacatcggc gaacatcgcg
    12301 tcgcattcgg ggcgcacctc ggcgtcgtgc cgccggagcg gaccgtaatg gccgagcgaa
    12361 ccgccgctca ccctcagcat caccgccgca tactcggtcg cgaagatggg gcaccgcttc
    12421 tcaagatcct ggaagatgac cccttcgccg gtgccgtagt tctcccaatc ggcgtcgcta
    12481 caatagacat cctctgaaaa gacctcgagc aggcatcggt ggttggcgaa ttcataggag
    12541 cccttccagt cggcgtagag tgtgtcgagt tcatcgctga atatgtggga gttgtaactg
    12601 gtctggaaga gccccgcctc cgccgtgtcg gcggtgacat tgtcagccga ctgatcgcgg
    12661 ccgcagcagt gttcgcccga actctcgcgc atgccgagcc cgaggaggag ggtgtaggcg
    12721 tggcgcaggg tgtcggcccc gtccgcgctg ttgtccatgc cgagggtgct gaatatctcg
    12781 tcgtaccacg cgagggcgtc atgttcggtg tcggcggtct tggcggcggc gaccagcgcg
    12841 acatcgctcc ggtcgcggtg gcagtaggcg ccggcgaaga cgagcgccat gccgcgggtg
    12901 taggcgggcg gcgcatcgcc gcgatcctcc cagtagtagt cggcgcactc cgagacatcg
    12961 gcgatgtcga tgacctcctg cgtgatatcg atacaatcgc cgaattcgcc ccactgaccg
    13021 ccggtgcatt cgcgtatctg cttgccgctc ccttcacagt cgagggattc ggtggcgccg
    13081 tcaacgcatt cggcgacaac ggcgtcctca tcggagacct catcgtccga cgcatccgac
    13141 tggtcgggcg taagagtatc ggtgtcgacc gactcgtcgg acggatcgga cagatccgtc
    13201 tgatcgtcag tgggttcctc gtcaggctga tcgtcgggcg cgctatcggc ggcgagcgtg
    13261 tcgccgtcaa cgagttcgct caggtcgacg catacttccg agacgcaggt aagcgtcccg
    13321 tcgcaggtgc cggtgtcgta gcaaggaccc ccttcggagc cgatcggttt gccgccgccg
    13381 gtatcacaag cggcgaagag gaacatcgta acggcgatca tgcatttttc tctcacgata
    13441 ggcctccttt cgatcggaac agatactcgg agtataggtg acgggaaggg aaaaacaatg
    13501 tttccgcaca ggaacgccta atgtttgact ttcgcgcgcg gcgcgtgtag aatcgccccg
    13561 ttcttttgtg acatctttgc ttggggaggc cctgatgaaa aggtcgttca ttctgcttct
    13621 tgtcccgctg ttgtccctga tgatcgccgg caccggctgc gatcagaaga aagggagcgg
    13681 gaccggcgag aaggtacaga cgaaggtcgc ggcggccgaa ggcggcaccg tcgagagttc
    13741 gtcgggcgag gtcaagatgg agatcccgcc gggcgcgctg tacgaggacg cctacatctc
    13801 cataacgctc gtcaacaagg aagggtatcc cgatagtggc aatgtcgcga gcaaagtggt
    13861 ccgctgcgag ccctccggcc tcaagttcaa ggaaccggtg actctcacca tcgtcgccga
    13921 cgcctccatc accgtccccg aaggacatcg gctcaccacc tcgatattcc gcaacgaaca
    13981 gtgggagaac cgtcattcgg gcgatcccat catggcgggc gaccccatca tggcgggcga
    14041 tcccatcatg gcgggcgacc ccatcatggc gggtaccggc tatacctcgc aggtaggcca
    14101 cttcaccgac ttcgccctct acctgcagga ggtgaccgag ggatccgacg acgatacgct
    14161 cctcgaggat gtcgatcaga ccacggacac ggtcgaggcg aaacccgaca ctgacgccgc
    14221 caccgatccg tgcgccggcg tgacctgttc cgggcacggc acctgctcg
//
LOCUS       DAOQUE010000007        14098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_4883_length_14098_cov_4.192124, whole genome shotgun sequence.
ACCESSION   DAOQUE010000007 DAOQUE010000000
VERSION     DAOQUE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14098
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4883_length_14098_cov_4.192124"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKH10_00455"
     CDS             complement(<1..209)
                     /locus_tag="PKH10_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26621.1"
                     /translation="MSTTGAQKIASTEENSRAQVINLSEEFFSKKPYQEEHEIAELLK
                     ELGAEDSHPVKKKKGGGLPTVVGVA"
     gene            complement(403..1812)
                     /locus_tag="PKH10_00460"
     CDS             complement(403..1812)
                     /locus_tag="PKH10_00460"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HNT26622.1"
                     /translation="MLGNVWLKIKVFFFISSVLAFLSFLFFNLFDHLVTRINFQQGAD
                     NTFRYLEQRIKNHQYELITRSFEISSKLKVTADKKTKQVVFDTQGIDPNLLFAFLDGN
                     GSLVTGSFRIFNSMVGVPAVDRAIRDGIASDGMLKTETERYLIGVAPFNVDLGLDRPK
                     RMIAVTLVPIREALSGVSVTFPAQVYTDNTLLYEFNKESLLKINSGEKQAEIQKAVED
                     ILAGNAPRTIDRWLHISSFSLPSDIRSAGGRTTFVGFMSALPGYEVYNKSVTYIGLYA
                     LAAIIITLIFTILITYDVDRGFKKLAADISRLRIGEKLVLHKYSHGAGIVVSSLNHLI
                     GNYQKHTESVGHGSSLSALVPEIKDEEVEIPEPSFDKKRESAPVTDTSKTVRTGGTDP
                     RIAQPVDNADPYAALWETYRGIKEKNGEKATDQERISFLAKLKTNRVSIMSKYNCRDV
                     SFVIEEKEGKPVIKAKPVK"
     gene            complement(1829..2566)
                     /locus_tag="PKH10_00465"
     CDS             complement(1829..2566)
                     /locus_tag="PKH10_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26623.1"
                     /translation="MLYAGKLTVTVTYAPSAVSREETMPETPIKDNYHQVPVLSPEKI
                     WEPEGYYAVLEGTDKGAPVDETAYLRGLSVNRSYFLLKVDGKLLLQNGESFTREFTVI
                     DDTGTVTKEFIVKPNTTAPYTFMRTGTYTFIDNRNPTCKIYVKVLAACVIFPVRGPSL
                     RIDLPVLDPGTYSFKIYYAFRHMFQEDFSMVGDSVQDVSYRITDREVFRSDSTTFTGS
                     RILSRSPVSDRPGPANAPNAGTSQQQP"
     gene            complement(2620..4773)
                     /locus_tag="PKH10_00470"
     CDS             complement(2620..4773)
                     /locus_tag="PKH10_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26624.1"
                     /translation="MTNKKNLFGFPKKTKESEEIDQYLALIKKEPDNDRNYIRLAELY
                     TRAGEEEKAIEVYEKVALLFEKKGFFNKAKAVLKQALAINPDHGRINVLLADLDKDDG
                     LIKDAVMRYQTAVNYFINTGNKAAATTILKKIVEIQPQNISYAIKLATLLVTEKMYHD
                     AEKILTPLMVSLKSTNKSADYVAVLKLLYLAKNENPDIGRELVKAYIKSGNHPQALSM
                     LHKLLLADPESTDLLEQLAFLFERMGEKEKLEAVYKQLASVHQERHATEESHKYYRKV
                     LELNPRDTEALLALNEEGRLRDIISGKIASASVDIAGDEHDEEEDEDIEIELDDHEKS
                     AISTPTVAPSPPQENFVPEKNEPAPPSETLQRSASSRQTIKEAQVFRSYRLFDRALER
                     LKAYPSWEKDVDILDLIAEIHIEKNDIRSGAETIFQLITVYLENGDEAHAKSIFYDAK
                     DMLEIMPETFQQMQEVLASMEEQPMMLSTPEDAPTAPPSPKVDHHTEAPLLLDPEEED
                     APSSPPAPSAADKEFADMARDLLTELNEIREPDQKRLDELEFFISIEDYQSATILLQE
                     LVSSYPESKLLVGFRDIIPLKKEENIADTLDEVKESLSKTMRGADQSAEDFYNMGIVH
                     LSMGMIAEAIGYFEQAAQMNSADPKYLIAISDAWFQSGKNAQSLKYAQKALEKATDND
                     SKAEILEKIANLYYSAGDTSRQQQALEEVKRLKRR"
     gene            complement(4795..5364)
                     /gene="efp"
                     /locus_tag="PKH10_00475"
     CDS             complement(4795..5364)
                     /gene="efp"
                     /locus_tag="PKH10_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNT26625.1"
                     /translation="MVSISTSEFRRGLKIEMEGVPYAIVDMVHIQQKRRAVIKTKLRN
                     ILSGSISEYTFLNGDKVEKPDLDERTMEYLYHDGDHYHFMDQESFEQITIDERIIEDA
                     IPFMRENERVLVQFYNGSPISVELPQFIELTIAETEPGYKGDTVTSSFKPAKVTTGGT
                     VQVPMFVGVGDVVRVNTAENFKYVERVKK"
     gene            5670..6704
                     /locus_tag="PKH10_00480"
     CDS             5670..6704
                     /locus_tag="PKH10_00480"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012862411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNT26626.1"
                     /translation="MKKYRIAVVGATGAVGRELLSILGERHFPIAEITLYASESSRGA
                     MVEYRGSEVPITVLSEPTAEDARHDIVFFCAGALVSSRFAPIFARHGAVVIDKSSAFR
                     MEPGVPLVVPEVNGHLLKKGLAEGYVVANPNCSTIQLVPYLQVLDDLFGLRSVVVSTY
                     QSVSGAGQKGIEELQRQTAELFSCRSAVPEVFPQRIAFNVIPHIDKFHPDGYTEEEKK
                     LINESRKILGRPDLDIDCTAVRVPTFYGHAESVTADLGCAADLDRLRAAISEKRGLAL
                     MDDPEKLVYPTPADVAGRDAIFVGRIRCGRSGADRARVQAWVVADNIRKGAALNGVQI
                     AETIIGKEQA"
     gene            6701..8833
                     /locus_tag="PKH10_00485"
     CDS             6701..8833
                     /locus_tag="PKH10_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26627.1"
                     /translation="MRKFTVVMVLLAATILAYAEDVSEISFDPAHREINNDGSLEIYF
                     DLPQLSAVEEVYIEVLVNGMTTVFPGSSRSNGDLSATGNRLSVNGSDLYDLAGGGVVF
                     SRSFAAGSDDDDDLLSDNEVTDEVTVPDEEGAHDDLLSDEDVIEQDDWPAERDGQYNF
                     NLVVVVDRDAVDDDAVPDDDIDVDTDTVTDNDTALVRQPLLYGTDETREPFTVRLDNV
                     PPAAPSAVKTESGNGKIYLTITRPEIDARGKSGESIGRYHVEMEGLFVYEGKEKRTTL
                     SFTFDLKESQRSDETATFTLEGKDGYALVNNDAGVDAYIYAIRVWAEDLAGNGDPGHS
                     IDATGSAATTEGFWSHYKSAGGKEKGDYCFIATVGYGDGRHPHVDLLRRWRDAFLMPY
                     AWGRAFVAAYYDHGPAIASVMWEAPILRPVVQALLFPLVLIAWLMMHPWLAGLLFLSP
                     VLLVCRSLSRRAVIISFLLCFLLLPGMTGASEKDEDDGAKDKKVAKTEKDEEKGEGEE
                     DDEITVHGNILFASGFYDPQYIDRDATGSPMGEILTSDLNYLPALHGGIDIPAGKYLK
                     LTAQAGIGFVELKGRTVRLDGTAGMERSYFYLLPLMAELKLRPVYEFPLRPYIAAGLD
                     YYIWWIIEDKHLVEDGGKFGLHGSVGIQLSLNFFDQKTAIKLREATGIIDTSLFAHYR
                     LERVDNFFAKNGFDLASSRFEFGILFDF"
     gene            8839..9639
                     /gene="truA"
                     /locus_tag="PKH10_00490"
     CDS             8839..9639
                     /gene="truA"
                     /locus_tag="PKH10_00490"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNT26628.1"
                     /translation="MFVLRLSFRGTGFHGWQMQVGVPTIQQTLRTAIERIFDTDQFPN
                     PSGCGRTDEGVHAIDFIATVKAARDIAPDNLRMGLNTLLPRSIRVLSVERRDGYRDAR
                     TLVAGKHYRYIICRTEVLSPFLDDLVWQSRYPLDIGPMRLAVAHLVGTHDFKSFQASN
                     TDVTETVRTIYHASLQERGPIIAIDIIGDGFLKHMMRILCGTLVGVAKGRIAPDDLPA
                     ILAARDRSRAGETLPGKGLYLYRLFEDGSLLRTTEPEMFSPDILWKCR"
     gene            complement(9651..11174)
                     /locus_tag="PKH10_00495"
     CDS             complement(9651..11174)
                     /locus_tag="PKH10_00495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT26629.1"
                     /translation="MREFLFYAYILLLFMLSVFGLHRYYMVWLYRKNINRRPEPKHCF
                     RDLPPVTIQLPIFNEKYVAERLIEAVAAIRYPSDRLEIQVLDDSTDETTLIAERKCGE
                     VRGRGIDIRYIHRDDRTGFKAGALENGLRTARGEYVAVFDADFLPDPDFLERTIHHFT
                     DPGIGMVQARWDHLNRGWSLLTESQSILLDGHFMIEHTARNRSGRFFNFNGTAGIWRK
                     TTIADAGGWQHDTLTEDLDLSYRAQIRGWRFVYLPDITVPAELPVEMSSFKSQQFRWA
                     KGSIQVFLKLYPSILRSALPAKVKMEAFFHLGANFAYLLMTLFCVLLPLNILLRHRHG
                     IREVIFFDLPVFLLATISVLFFYFYSEMAVIRSTAHVVHASKLRLLFYLPATMAVGIG
                     LTANNACAVIEALIRKTSPFVRTPKYDITGSGRGNGRLAKVLANAYRSKTINGVALIE
                     ALFAVYFTIGIHAAFLAGFWFSIPFLLLFQFGFLYTAVMTLLGEPIAALLRRLNPTP"
     gene            complement(11252..12220)
                     /locus_tag="PKH10_00500"
     CDS             complement(11252..12220)
                     /locus_tag="PKH10_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26630.1"
                     /translation="MKELLTILLALATALILSSCGEEEDPEEVNDLPDWDYVSSSDNA
                     AGIDDAPTTLDEEEAPDEATTDQATGPDDAPTAWCGNGFIEPAERCDLSPSDCAKINP
                     GLTNIANCKPDCSGWSMADCSKNTKVWGVMTLDFRTDYILNESRLNDPVYCEQGLQHH
                     DAFFGMYGDNTPIPAATATVTWSAASNTPAGNGLYTTFVRQHSFNGTTEIFPYIEIEF
                     PPGAMTLADYSVNSTDTIFFNKRMVRVRLIGVNGGSPCVAAMGFWGTVNVEDAWQIDP
                     AEGGRIALVSANIEFYPPRELPNLALEEGDPLPDDLLSIMKYPECP"
     gene            complement(12222..13001)
                     /locus_tag="PKH10_00505"
     CDS             complement(12222..13001)
                     /locus_tag="PKH10_00505"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNT26631.1"
                     /translation="MRRYDIERTPWGNFYHFAELDSTNAEAIRLIEKGISSGIVIADR
                     QTAGRGCGGNGWFSPKGRNLYVSFFSRTEMDRSRDLAKMAGVALYETIAGVIPTTRLL
                     LKWPNDILADRKKVAGILIQQVRRGPALLAVTGIGINIETPDKQAFAWRWEPSSLEET
                     AGTSVSPNTVLRRLVSSLEHWRHAAGNEIDTRYDELVAWMTGRTTRWLDGGAERQGTI
                     RRFADRHLTLIIATDNGEELALSAGEVTGLQIPGDDPATEV"
     gene            complement(12998..13732)
                     /locus_tag="PKH10_00510"
     CDS             complement(12998..13732)
                     /locus_tag="PKH10_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNT26632.1"
                     /translation="MSFFERTAIIYGPAASARLSRATVALFGLGGVGAAASVDLVRAG
                     VGHLVVTDFDAVQESNLNRLAIGFTDTVGQEKVTVLRDTAHRINPAVIIEGHARFLRG
                     ASVADNIPEADVYLDAIDALNPKVNLIAALLASGRPFLSVLGTAGRRDPTRLQVADIW
                     STHGCPLGYFVRKRLRKQGIDGRFPVIFSPEPPVEPVENPDQRDDGKGRIRRIQGSTP
                     FVPQAAGHIAAWWLVEKLLSSQRGAS"
     gene            13840..>14098
                     /locus_tag="PKH10_00515"
     CDS             13840..>14098
                     /locus_tag="PKH10_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26633.1"
                     /translation="MKRMMSALLLLAAVPVLCAAEAPVPINDPVPTSPVAARKLLVDK
                     IEAVEVEEAFARSLEEALVLDIGKREGFSVVTSAEMSATAMS"
BASE COUNT         2848 a         3725 c         4271 g         3254 t
ORIGIN      
        1 aaggccacgc ctacaactgt cggcagccct cccccttttt tcttcttgac aggatgcgaa
       61 tcctccgcac cgagttcctt gaggagctcg gctatctcgt gttcttcctg gtacggcttc
      121 ttgctgaaga actcttcaga gaggttgatc acttgagcgc gactattctc ttcggtcgac
      181 gcgatctttt gcgcaccagt ggtgctcaag ggccgatcgg tgtttgaccg agcatgctcc
      241 cgcggggttt cttcgatgga tatcgaagtt ttttgcgggg ccgactcgac aatggaggta
      301 gagcattcgg tgttggagct tccgcagagg gggcaggtct ctatcttttg gggtatctcg
      361 gcggaaaaac cgcaattgtt gcagatgaaa ttcatgaact gtttatttga ccggtttcgc
      421 tttaatgacc ggtttcccct ctttctcctc aatgacgaag gaaacatccc gacagttgta
      481 tttactcatg atggaaaccc ggttagtttt caatttcgcg agaaaagaga ttctttcctg
      541 gtccgttgct ttttccccgt ttttctcctt gatgccacga taagtctccc aaagagcggc
      601 ataggggtca gcgttgtcga ccggttgcgc gatcctcgga tcggtgccgc cggtgcgcac
      661 ggtctttgat gtgtccgtga cgggggccga ttcccgtttc ttgtcgaaag agggttcggg
      721 tatctccacc tcttcatcct ttatttcggg gaccaaggcg gagagagaag atccatgccc
      781 caccgattcg gtatgtttct ggtagttgcc gataaggtgg ttcagcgatg agacaacgat
      841 ccctgcgcca tggctatact tgtgcagtac cagtttctct ccgatcctga gccgggagat
      901 atcggcggcg agtttcttga atccgcggtc gacatcatag gtgataagga tagtgaaaat
      961 gagagtgatg atgatggccg cgagcgcata caggcctatg taggtgacgc ttttgttata
     1021 gacttcgtag ccgggcagcg cgctcatgaa tcccacaaag gtcgttctgc cgccggccga
     1081 gcggatatcg gaaggcagag aaaaagaaga gatgtgaagc caccggtcga tagtgcgggg
     1141 agcatttccc gcaagaatat cctctactgc cttctgtatc tccgcctgct tttcacccga
     1201 attgatcttc agaagggact ccttgttgaa ttcgtatagt agggtgttgt ccgtgtagac
     1261 ctgcgccggg aaggtcaccg atacgccgct caacgcttcg cggatgggga caagggtcac
     1321 cgctatcatc cgtttcggtc tatcaagtcc cagatcgaca ttgaaggggg ctacgccgat
     1381 gaggtatctt tcggtctcgg ttttcagcat gccgtcggaa gcgatcccgt cgcggatggc
     1441 gcggtcgacg gccggcactc ccaccatgga attgaagata cgaaaactgc cggtcacgag
     1501 agaaccgtta ccgtcaagaa acgcaaaaag gaggttgggg tcgatccctt gcgtgtcgaa
     1561 gaccacctgt ttagtctttt tatcagcggt gaccttgagt ttcgaagata tctcgaagga
     1621 gcgggtgatg agttcgtact ggtggttctt tatgcgctgc tcgaggtacc ggaaggtgtt
     1681 atcggctccc tgttggaagt ttatcctggt gaccagatgg tcgaaaaggt tgaagaacag
     1741 gaaggaaagg aaggcgagaa cgctggagat gaagaagaac accttgatct tgagccagac
     1801 atttcccaac ataacagccc ctccgtgatt atggttgctg ttgcgatgtg cccgcattcg
     1861 gggcgttagc cggtcccgga cgatcagaaa cgggagagcg ggagaggatg cggcttccag
     1921 taaaggtcgt cgagtcacta cggaagacct cgcgatcggt tatccggtag gagacatcct
     1981 gcaccgagtc cccgaccata gagaaatctt cttggaacat atgtcggaaa gcatagtaga
     2041 tcttgaagga gtaggtgccg gggtccagca ccggcaggtc tattcgcaaa gaaggtccgc
     2101 gcaccgggaa tatcacgcag gctgcgagca ccttgacgta gatcttgcag gtcggattac
     2161 gattgtcgat gaaggtgtag gtgccggtgc gcatgaaggt gtagggcgca gtggtgttcg
     2221 gtttgacgat gaattctttg gtcacggtgc cggtgtcgtc gatcacggtg aattcccgcg
     2281 tgaacgattc tccgttctgc agaaggagtt tcccgtcgac cttaagaaga aaatagctgc
     2341 gatttaccga aagaccgcgg aggtaagccg tttcgtcgac cggggcgccc ttatcggtgc
     2401 cctccagcac ggcataatac ccttccggtt cccatatttt ttcgggggag agcaccggta
     2461 cctgatggta gttgtccttg atcggggtct ccggcatggt ctcttcgcgg gagaccgcgg
     2521 aaggcgcgta ggtgacggtg accgtcagtt tcccggcata caacatgacg ggaaagcaaa
     2581 tcgggatcag ggaaagaaga cccaaagacc ctttcacgct catctccgtt tcagtctttt
     2641 tacctcttcc agcgcctgtt gctggcgact ggtgtctccc gcactgtagt aaagattcgc
     2701 gatcttttca agaatttccg cttttgaatc gttatcggtc gccttttcga gcgccttttg
     2761 agcatatttg agactctgcg cgttcttgcc gctctggaac cacgcatcgg atatggcgat
     2821 caggtacttc gggtcggcac tgttcatctg cgccgcctgt tcgaaatagc cgatggcctc
     2881 agctatcatg cccatcgaga gatggacgat ccccatgttg taaaaatcct cggccgattg
     2941 gtcggcgccc cgcatggtct tcgatagcga ttccttgacc tcgtcaagtg tgtcggcgat
     3001 gttctcttct tttttcaggg ggatgatgtc gcgaaaaccg acaagcagtt tcgactccgg
     3061 gtaactggac accagttcct gtagaagtat cgtcgcgctt tgatagtcct ctatgctgat
     3121 gaagaattcc aattcatcca gtcgtttctg gtccggttcg cgtatctcgt tcagttcggt
     3181 cagcaggtcg cgtgccatat cggcgaattc cttgtcggcc gccgaaggtg ccggcggtga
     3241 tgaaggagca tcttcttctt cgggatcgag aagaagcggg gcctcggtat ggtgatcgac
     3301 cttcggggaa ggaggagcgg tcggggcgtc ctccggtgtc gaaagcatca tgggctgttc
     3361 ttccatgctc gcgagaacct cctgcatctg ctggaaggtt tcgggcatga tctccaacat
     3421 gtctttcgcg tcgtaaaata tgctcttggc gtgcgcttcg tcgccatttt cgagataaac
     3481 cgttatcaac tggaaaatgg tctcggcacc tgaccggata tcgttctttt cgatatgtat
     3541 ctcggcgata agatccagta tgtcgacatc cttttcccaa gatggatagg ctttcagtct
     3601 ttccagtgcg cgatcaaaaa gacggtaact gcggaagacc tgagcctctt tgatggtctg
     3661 gcgcgaggat gcggagcgtt gtaaggtctc gcttgggggt gccggctcat ttttttccgg
     3721 aacaaaattc tcttggggag gggacggagc gaccgttggg gtcgatatcg ccgatttttc
     3781 atgatcgtca agttctatct cgatatcttc atcttcttcc tcgtcgtgtt cgtctcccgc
     3841 gatgtcgacc gaggcggaag ctatctttcc cgaaatgata tcgcgaaggc gtccctcctc
     3901 gttaagtgca agaagcgctt cggtgtcgcg cgggttgagt tcgagtacct tacggtaata
     3961 tttatggctc tcttcggtcg catgcctttc ctgatgaacg gatgccagtt gcttgtagac
     4021 cgcctccagt ttctcttttt ctcccatccg ttcaaaaaga aatgctagtt gttcgagcag
     4081 gtcagtggat tcgggatcgg cgagaagcaa cttgtgaagc atggagagcg cctgagggtg
     4141 attcccgctt ttgatgtagg ccttcaccag ttcgcgaccg atgtcgggat tctcgttctt
     4201 ggcaagatac agcagtttga ggacggcgac ataatcggcg cttttgttgg tgctcttcag
     4261 cgagaccatg agcggcgtca ggattttttc cgcatcgtga tacatttttt ccgtgaccag
     4321 gagcgtagcg agttttatcg cataggagat gttctggggc tggatctcga ctatcttttt
     4381 aagtatggtg gtggcggccg ccttgttgcc ggtgtttatg aaataattca cggcggtctg
     4441 atagcgcatg accgcgtcct taatgagtcc gtcatccttg tcgagatcgg cgagaagcac
     4501 attgatgcgc ccatggtcgg gattgatggc aagcgcctgt ttgagtacgg ccttggcctt
     4561 gttgaagaac cccttctttt cgaaaagaag agcgaccttc tcgtagacct cgatggcttt
     4621 ttcctcttcg ccggcgcggg tgtatagctc cgcgagacgg atatagttgc ggtcgttgtc
     4681 gggctctttt tttatgagtg cgaggtactg atctatctct tcggattcct tcgtcttttt
     4741 ggggaaccca aacaggttct ttttattggt cacttcgccc aaaacaggcc ccggtcactt
     4801 cttaacgcgt tcgacatact tgaaattctc ggcggtgttc acgcggacaa catcaccgac
     4861 gccgacgaac atgggtacct gcaccgtccc tccggtggtg acttttgctg gtttgaagga
     4921 actggtgacg gtgtcccctt tgtagccggg ttcggtctcc gctatcgtga gttcaatgaa
     4981 ttgcggtagc tcgaccgaga tgggggagcc gttgtagaac tgcacgagga cccgttcgtt
     5041 ctcacgcatg aagggaatgg catcctcgat gatccgttcg tcgatggtta tctgttcaaa
     5101 actttcttga tccataaagt gataatggtc gccgtcatgg tacagatact ccatggtgcg
     5161 ttcatcaagg tccggcttct cgaccttgtc gccgtttaaa aaagtgtatt cggatatgga
     5221 gccggaaagg atattgcgta actttgtctt gatgacggcc cgccttttct gctggatgtg
     5281 caccatgtcg acgatcgcgt agggcacccc ttccatctct atttttagtc cacgccggaa
     5341 ctcggatgtc gatatcgaaa ccatgaaaag acctccgtac gcagggaaac aaaaatctta
     5401 atcgcggtaa tgttatatgc cgatgtttca ttgtcaagat aaaacggcat cgaaaaagat
     5461 ggcacgaacg atccgggttc tcgatggtcc ggaaaaggtg acagtttttc gtgtcagagt
     5521 ggcatcttgt cggtaaacag aagatgtttt gtttgtaatc gtatggaaag attgacgaat
     5581 aaaaacggca tacgagttgc atatcgcgcc ccttttctgt aatatcggcg gtcgatacga
     5641 ggcgttagtt ccgagcagga ggtacacaga tgaagaaata tcgcatcgcg gtggtcggcg
     5701 cgacgggagc ggtcggccgg gaattgctct ccatccttgg tgagcgccat tttccgatag
     5761 cggagatcac gctgtatgcg agcgagagtt cacggggtgc gatggtcgaa taccgcggct
     5821 cggaagtgcc gatcacggtc ctttcagagc cgaccgccga agatgcgcgg cacgacatcg
     5881 tctttttttg cgcgggtgcg ctcgtttcct ctcgtttcgc gccgatcttt gcccgtcacg
     5941 gcgccgtggt catcgataag agttccgcct ttcgcatgga accgggagtg ccgcttgtgg
     6001 tgcccgaggt caacggtcat ctcctgaaaa agggccttgc ggaaggatat gtcgtggcaa
     6061 acccgaactg ctcgacgata cagttggtcc cgtaccttca ggtgctggac gatctgttcg
     6121 gtctcaggtc ggtcgtagtg agcacctatc agagcgttag cggcgccgga cagaagggca
     6181 tcgaggaatt gcaacgccag acggccgagc tgttttcctg cagaagtgcg gtccccgagg
     6241 ttttccctca gaggatcgcg ttcaatgtca tcccgcacat cgacaagttc catcccgacg
     6301 gttacacgga agaggagaaa aagctcatca acgaatcccg caagatactg ggcagacctg
     6361 acctcgacat cgattgcacg gcggtgcgcg tgccgacctt ttatggccat gccgagtcgg
     6421 tgaccgccga tctgggatgt gccgccgacc tcgatcggtt gcgtgccgct atcagcgaaa
     6481 agaggggact tgcgcttatg gatgatccgg agaaactggt ttatcctacg ccggccgatg
     6541 ttgcagggag ggatgccata ttcgttggcc gtatcaggtg cgggcggtcc ggtgcggatc
     6601 gcgcgagagt gcaggcgtgg gtggtcgccg acaacatcag gaaaggtgcg gcgcttaacg
     6661 gggttcagat cgccgaaacg atcatcggca aggagcaggc atgagaaagt tcaccgtggt
     6721 gatggtattg ctggcggcaa cgatccttgc ctatgccgag gatgtttccg agatatcgtt
     6781 cgatccggcg caccgagaga tcaataatga cgggtccctc gagatatact tcgatctccc
     6841 gcaattgagc gcggtcgaag aggtctatat cgaggtcttg gtcaatggca tgacgacggt
     6901 gttccccgga tcctcgcgta gtaacgggga cctttctgcg accgggaacc gtctttcggt
     6961 gaacggatct gatctttatg atcttgccgg cggcggcgtg gtcttttccc gatcgttcgc
     7021 cgcaggatcg gacgatgatg acgatctcct ttcggacaac gaggtgaccg atgaggtgac
     7081 cgtgccggat gaagaaggcg cccatgacga cctgctatcc gacgaagatg ttatcgaaca
     7141 ggatgactgg ccggcggagc gagatggtca gtacaatttc aatctggtcg tcgtcgttga
     7201 ccgggatgcc gtcgatgacg atgcggtccc tgatgacgat atcgatgtcg acaccgatac
     7261 cgtcaccgac aacgatacgg ctctggtcag acagcccttg ttgtacggta cggatgaaac
     7321 gagagagccg ttcaccgtcc gtctcgacaa tgtgcccccg gccgcccctt cagccgtcaa
     7381 gaccgaaagc ggtaacggga agatatacct cacgataacg cgaccggaga tcgatgcgcg
     7441 gggaaagagc ggcgaatcga tcgggcgtta ccatgtggag atggaaggcc ttttcgtcta
     7501 cgagggaaag gagaaaagaa cgaccctctc cttcaccttc gacctgaaag agtcccaacg
     7561 gtccgacgag acggcgacct tcacccttga aggtaaggac gggtacgcgc tcgttaacaa
     7621 tgatgccggc gttgacgcgt atatctacgc catcagggtg tgggcggaag atcttgccgg
     7681 gaacggcgat ccgggccatt ccatcgatgc gaccggaagc gcggcgacca cggagggatt
     7741 ctggagccac tacaagtcgg cgggcggaaa ggagaagggg gactactgtt tcatcgcgac
     7801 cgtcggatat ggggatggca ggcatccgca tgtggacctg ttacgcaggt ggagagatgc
     7861 ctttttgatg ccatatgctt gggggcgtgc cttcgtcgcg gcatattacg atcatggccc
     7921 ggcgatcgct tcggtcatgt gggaagcccc gatactgcgg ccggtcgtgc aggcactctt
     7981 gttcccgttg gtgctcattg cctggctcat gatgcaccct tggttggcgg gacttctgtt
     8041 cctgtcgccg gtcctgttgg tgtgccgatc cctttcccgt cgcgcggtca tcatatcctt
     8101 tcttctgtgt tttttgttgt tgcccgggat gaccggcgca tcagagaaag acgaggatga
     8161 tggagcaaag gacaagaaag tagcaaagac cgaaaaggat gaagagaagg gcgaagggga
     8221 agaggatgac gagataacgg tacacgggaa tatccttttt gccagcggtt tttacgatcc
     8281 ccaatacatc gatcgtgatg cgaccggttc cccgatgggc gagatactca cctccgacct
     8341 gaactatctt ccggcgcttc atggcgggat tgatatcccg gcggggaaat atctcaaact
     8401 caccgcgcag gcgggtatcg ggttcgtcga actcaagggg agaacggtgc gccttgacgg
     8461 caccgccggc atggaacgga gttactttta cctcctcccg ctcatggccg aactgaaact
     8521 gcgcccggtc tacgagttcc cgctccgccc ctatatcgcc gccggtctcg attattacat
     8581 ctggtggatc atcgaagaca aacatcttgt cgaggacggg gggaagttcg gtctgcacgg
     8641 ttcggtgggc atacagttgt cgctgaattt ctttgatcag aagaccgcga taaaactgcg
     8701 ggaggcgacc ggcatcatcg acacctcgct ttttgcgcac tatcgcttgg agcgggtgga
     8761 caatttcttt gcaaagaacg gattcgacct tgcgtcgagt cggttcgaat tcggcatcct
     8821 tttcgacttt tgacgccgat gttcgttcta cgactttcct tccgcggcac cgggttccac
     8881 ggctggcaga tgcaggtcgg agtgccgacc atacagcaga ccttgcggac ggcgatcgag
     8941 aggatattcg acaccgatca attcccgaat ccttcggggt gcggtcgcac cgatgagggg
     9001 gtgcatgcga tcgacttcat cgcgacggtc aaggcggccc gcgatatcgc ccccgacaat
     9061 cttcgtatgg ggcttaatac cctgctcccg cgctccatcc gggtcctttc ggtcgagcgg
     9121 cgtgacgggt atcgcgacgc ccgaacgctc gtggccggga aacattaccg gtatatcatc
     9181 tgccggaccg aggtgctttc accgttcctt gatgaccttg tctggcagag ccgctatccg
     9241 ctcgatattg gaccgatgcg cctcgccgtg gcacaccttg tcggcacaca tgacttcaag
     9301 tcgttccagg cgtctaatac cgatgtgacc gaaacggtcc ggaccatcta tcatgcttct
     9361 cttcaggagc gggggccgat catcgccatc gacatcatag gcgacgggtt cctcaaacat
     9421 atgatgagga tcctttgcgg gacgctggtg ggcgtagcga aggggcgtat cgcgccggat
     9481 gaccttcccg ccatcctcgc ggcgcgcgac cggagccgtg ccggcgaaac actccccggt
     9541 aaggggctgt atctgtatcg tctgttcgag gacggatcgt tgcttcggac gacagagccg
     9601 gagatgttct ctcccgacat cctgtggaag tgcaggtagt gtgcccgccc ttagggggtc
     9661 ggattcagac ggcgtagcaa ggccgcgatc ggttctccga ggagggtcat caccgcagta
     9721 tacaggaagc cgaactggaa caggagcagg aagggtatgc tgaaccagaa gccggcaagg
     9781 aaggcagcgt ggatccctat cgtgaagtag acggcgaaga gggcctcgat gagcgccaca
     9841 ccgttgatgg tctttgagcg ataggcgttc gcaagcacct tggccagccg accgttccct
     9901 cgtccggaac ccgtgatgtc gtatttcggg gtccgtacga acggggatgt cttccgtatc
     9961 agggcttcaa taacggcgca cgcattgttg gcggtcagtc cgatgccgac cgccatggtt
    10021 gccggcagat agaacagcag gcgcagtttc gaggcatgca ccacgtgggc cgtcgagcga
    10081 atgaccgcca tttcggaata gaaataaaag aacaacaccg aaatggtagc gagcaggaat
    10141 accggtaaat cgaagaagat gacctcgcgt atcccgtgcc ggtggcgcag aaggatgtta
    10201 agcggaagca ggacgcagaa aagggtcatc agcagatagg caaagttagc gcccagatgg
    10261 aaaaatgctt ccatcttgac cttagcgggg agcgccgagc gcaggatgga tggatacagc
    10321 ttgaggaata cctggatcga acctttcgcc cagcggaact gctgtgattt gaaggatgac
    10381 atctcgaccg gcagttctgc cgggaccgtt atgtcgggca gatagacgaa tcgccatccc
    10441 cgtatctggg cacggtagga gaggtcgaga tcctcggtca gcgtatcgtg ttgccagccg
    10501 cccgcgtcgg cgatggtggt cttgcgccag atgccggcgg tcccgttgaa attgaagaag
    10561 cggccgctcc ggttgcgtgc cgtgtgctct atcatgaaat ggccgtcgag caggatgctc
    10621 tggctctccg tgaggagaga ccacccgcgg ttcaggtgat cccatcgcgc ctgtaccatc
    10681 ccgatgcccg gatcggtgaa gtggtgtatc gtccgctcga ggaaatcggg gtcgggcaag
    10741 aaatcggcgt cgaagacggc gacatattcg ccgcgtgcag ttcggaggcc gttctcgagg
    10801 gcgcccgcct tgaagccggt ccggtcatca cggtggatat agcgaatgtc gatgccgcgt
    10861 ccacgcactt cgccgcattt tcgctccgcg atgagggtcg tttcgtcggt ggaatcgtcg
    10921 agtacctgta tctcgagccg gtcggacgga tagcgaatgg cggcgacggc ctcgatgagt
    10981 cgttcggcga catatttctc gttgaatatc ggcagttgga tggtcactgg cggcagatcg
    11041 cggaagcaat gcttcggttc ggggcgtctg ttgatattct ttcggtaaag ccacaccatg
    11101 tagtaacgat ggagaccgaa caccgaaagc atgaaaagaa ggagaatata ggcataaaag
    11161 aggaactctc tcatgcttta tgacctatta cacggtgaat aacatacgag gcattcttta
    11221 cgccggaaga aaactttgtc aaattttagg gttagggaca ctcgggatat ttcatgattg
    11281 aaagaagatc atcggggagc gggtcacctt cctcgagcgc gaggttgggc agttcgcgtg
    11341 gcgggtagaa ctcgatattg gccgacacga gcgcgattcg cccgccttcg gccgggtcga
    11401 tctgccacgc atcttcgaca ttgacggtcc cccaaaagcc catcgccgca acacaggggg
    11461 accctccatt gacgccgatc aagcgaacac ggaccatccg cttgttaaag aagatggtat
    11521 cggtgctgtt gaccgaatag tcggcgagcg tcatcgcgcc gggggggaac tctatctcga
    11581 tgtaggggaa tatctcggtg gtcccgttga aggaatgctg gcgcacgaag gtggtgtaga
    11641 ggccgttgcc cgccggggtg ttcgaggcgg cgctccaggt gacggtcgcc gttgcggccg
    11701 gtataggggt gttgtcgcca tacatcccga agaaggcatc gtggtgctgg agtccctgtt
    11761 cgcaataaac agggtcgttc aaacgcgatt cgttaaggat atagtcggtc ctgaagtcga
    11821 gcgtcatgac gccccagacc ttggtgttct tgctgcagtc ggccatggac caacccgagc
    11881 agtcgggttt gcagttcgcg atattggtga ggccggggtt tatcttagcg cagtcgctcg
    11941 gggagaggtc gcaccgttcg gccggctcga tgaaaccgtt gccgcaccac gcggtagggg
    12001 catcgtcggg accggtcgcc tggtcggtcg tcgcttcatc gggcgcctct tcctcatcga
    12061 gggtcgttgg cgcgtcatcg atgccggcgg cgttgtcgct cgatgagaca tagtcccaat
    12121 cgggcaggtc gttcacctct tcagggtcct cttcctcgcc gcaggacgag aggataagag
    12181 cggtcgccag agcgagcaag atcgtcagca gttctttcat atcacacctc cgtggcaggg
    12241 tcatccccgg gtatctgcag gccggtcacc tctccggccg aaagcgccag ttcctctccg
    12301 ttgtccgtgg cgatgataag ggtcagatgc cggtcggcga accggcgtat cgttccctgc
    12361 cgttccgcgc cgccgtcgag ccacctcgtc gttctcccgg tcatccacgc gaccagttcg
    12421 tcgtagcgcg tatcgatctc gtttcccgcc gcatgacgcc agtgttcgag cgaagagacg
    12481 agtctcctga gcacggtgtt cggcgatacg gatgtgcccg ccgtctcttc gagcgacgat
    12541 ggttcccagc gccaagcgaa cgcctgtttg tcgggggtct cgatgttgat gccgatgccg
    12601 gtgaccgcca gcagtgcggg gccccgcctg acctgttgga tgagtatgcc ggcgaccttc
    12661 ttccggtcgg cgagtatgtc gttcggccac ttgaggagca gtcgggtcgt cgggatgacc
    12721 cccgcgatcg tttcgtagag cgcgacgccg gccatcttgg cgagatcgcg cgaccgatcc
    12781 atctcggtgc gcgagaagaa ggacacatag aggttcctgc ccttcggcga gaaccagccg
    12841 ttgccgccgc agccgcggcc tgccgtctgc cggtcggcga tgacgatgcc tgacgatatc
    12901 cctttctcga tgaggcggat cgcctcggcg ttggtcgaat cgagttcggc gaaatggtag
    12961 aaattgcccc acggcgtcct ctcgatatcg taccgcctca tgatgctcct cgctgagagg
    13021 atagtagttt ctcgacaagc caccatgcgg cgatgtgccc ggccgcctgc ggcacgaagg
    13081 gggtggatcc ctgtatccgg cggatgcgcc ccttgccgtc gtcgcgctgg tcggggttct
    13141 cgaccggttc caccggcggt tcgggagaga atatgacagg gaatctgcca tcgataccct
    13201 gcttgcggag ccgtttccgg acgaagtacc cgagcgggca gccgtgagtg ctccagatgt
    13261 ccgccacctg cagtcgcgta gggtcgcgtc gccccgcggt gccgagtacc gacaggaacg
    13321 gcctcccgga ggcgagaagg gcggcgatga ggttcacttt cgggttgagg gcatctatcg
    13381 cgtcgaggta gacatcggcc tccgggatgt tgtcagcgac cgaggcgccg cgcaggaaac
    13441 gggcgtgtcc ttcgataatg accgccgggt tgatacggtg cgccgtatcg cgcagtacgg
    13501 tcaccttttc ctgccccacc gtgtcggtga acccgatggc aagccggttg agattgcttt
    13561 cctggaccgc gtcgaagtcg gtgaccacca gatgcccgac accggcgcgt accagatcga
    13621 ccgaggcggc ggctccgacc ccgccgagcc cgaacagggc cacggtcgcc cgggaaagcc
    13681 gcgccgacgc cgcggggccg tagatgatgg ctgtccgctc aaaaaatgac atagtatctc
    13741 ctcgacctgc gatccatgat acacgggaga gatatttttt gcaatttcgt gaaagagcga
    13801 taccatccgg gcgtttctga tctcagggag ggatggccca tgaagcggat gatgagcgcg
    13861 cttttgttgc ttgcggcggt tccggtgctt tgcgccgccg aagcccccgt gccaattaac
    13921 gaccccgtcc cgacaagccc ggttgcggcg cgcaaactgc tcgtcgacaa gatagaggcg
    13981 gtcgaggtcg aagaggcgtt cgcgcggtcg ctcgaagagg cgctggtcct cgacatcggc
    14041 aaacgcgaag gtttcagcgt cgttacctcg gccgagatga gcgcgacggc catgagcg
//
LOCUS       DAOQUE010000008        14025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_4915_length_14025_cov_4.383107, whole genome shotgun sequence.
ACCESSION   DAOQUE010000008 DAOQUE010000000
VERSION     DAOQUE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4915_length_14025_cov_4.383107"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..184)
                     /locus_tag="PKH10_00520"
     CDS             complement(20..184)
                     /locus_tag="PKH10_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26634.1"
                     /translation="MFEKPTLIISHSFPLSPDDYRGRFIADMLPQDGAVPVVVLTPDP
                     EGPGVDTMEQ"
     gene            complement(189..1157)
                     /locus_tag="PKH10_00525"
     CDS             complement(189..1157)
                     /locus_tag="PKH10_00525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNT26635.1"
                     /translation="MSDPLVSVILPAYNAERHIGVAIRSILTQSFSNFELVVVDDGSS
                     DGTAEEIGKVDDPRIRLLRNDRNQGLIFSLNRGIDEAHGRYLARMDADDISVPRRLEE
                     QVSFMEAHPEVGLCGSWAQKFIPFGPRWTQKAPRNSGALKAGLLFATPFVHPSVMMRR
                     SVLAEHGLRYREGYETAEDYRLWCEMALVTELAVIPEVLLRYRVGLGSVTGRVLSDRG
                     RSMRRREVLKRIWGQFIEKTMGFMPDEAQLDAHARFYDARFTTISPEDRRQAIRWLDQ
                     VREKSPEWCFFDEAALVMACDRAARSLDDRSPVVFLRRILTAFFSR"
     gene            complement(1154..1987)
                     /locus_tag="PKH10_00530"
     CDS             complement(1154..1987)
                     /locus_tag="PKH10_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HNT26636.1"
                     /translation="MITVRLCGGLGNQMFQYAAGRALSLRLEQPLFLDLSWFSELSKD
                     TPRPFDLSRYQIIAAWGQPGLWGRLRCRITGSTHVFAERDLQFDQSFHSLTDPVSLRG
                     YFQSERYFSDIRETLLAEFVPREPLSEEDALLEKEMSSRASLALHVRRGDYAHNPTTR
                     AFHGLLGGAYYEKAVDFVATKVSIEDIYIFSDDMPWVQENLRFDRPVRNVSTGSAVHD
                     MYLMSSCKHQIIANSSFSWWGAWLNHNPEKIIVSPKRWFANELAERQTGDLIPGGWVR
                     I"
     gene            complement(1984..3618)
                     /locus_tag="PKH10_00535"
     CDS             complement(1984..3618)
                     /locus_tag="PKH10_00535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT26637.1"
                     /translation="MQNFQVTDIRWASEGELSLPLWRDWNKKNKISQKSWQEVWAGPY
                     FSSKVPPSAGNTSFSPTSGDRGDEGGSDYLTLLSKRRMVADLVEDLKKMMRIARHRVV
                     VVVPVSDQSGLPVIDLQVLEDFLGCHIPLLPFNALRADSSVVRKYKHMHDTKNRHTRL
                     LAITLDAIDAPRSVAVLIPHWESYRFLKPCLTHIVKNRDPLLKEKIYVLDDESQDGSF
                     DLARKEFARFPFIEFYQIRRHNKKNAADIGLLLDEGLKLVSEQFSVMLDADTLPLSVD
                     WIAFPIWLLEKYRSVSVGLDTGLSNSYYQDAWGSPLWQPESGYMPGFALYDNEWFSCT
                     NNLYRVMRTADARVVSEGIGFTRANSESRTVEHYFRDLVALFERHGFSFKNIYRALFQ
                     KKCPILQNLAEDRYPYLRKGCDNGVAANHFMDINRLGPKINIPITSFIGLTPHDGVFG
                     QNIGGLLFHFALSTRALSRERREVFEVGNLYEKWVESLSSDDASFAAILEEMIAASAS
                     FKPGGYDGKIPADWYRSQYERVRQLQERYRTERSAR"
     gene            complement(3596..4291)
                     /locus_tag="PKH10_00540"
     CDS             complement(3596..4291)
                     /locus_tag="PKH10_00540"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT26638.1"
                     /translation="MTNFLRAVNSWDQAFAKAAQNHSMQNLQISRDFFSEIRAPLWGV
                     GRHVPFRRAIEQSRSIFEFGCGTGDLSKMLRKYTKAEYLGVDISQNAIDYANARYSNK
                     RTLFMMRNILETPLSPDMRYELAIASNVLEHFLDPWNVCDVLLSFSKQLIVLAPYKQP
                     VTDGYEVEGGLGHVFCFDEIGFQLRYCLLDWFVFKTAGWTYSSRGEIPQQMAVLITKK
                     DGAGEEDAKFPSN"
     gene            complement(4288..5499)
                     /locus_tag="PKH10_00545"
     CDS             complement(4288..5499)
                     /locus_tag="PKH10_00545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT26639.1"
                     /translation="MQEVVSQISERLVRMGHEVTVATSTNPERVEKIINGVRVEGFAI
                     SGNMVNGYGGDLAEMERYQHLLREGDFDIVTNFAAQQWATDLTLPILDEIKARKVLVP
                     TGFSGLFLGSYRDYFEKMPGWMRKYDMNVFLSDTYRDARFAEEHGIENRVLIPNGASE
                     EEFEAEAAFSLRERLGIPADAFLVLLVGSHTGMKGHREAVRIFDAAQVSNAVLLIVGN
                     YCDTVAPAFSVRTWARDVARRLLRRAQDECPRFCRAAERNFNGMEHWRSAGKRLLFAD
                     LERCATVMAYREADLFLFPSRVECSPIVLFEAAASRTPFLATDVGNAAEIAAWTGGGA
                     ILPTSHDRKGYSQADIGGSAAMLEELWRDADRRRRMADAGRAAWRERFTWESVATRYF
                     QLYQTLITSKS"
     gene            complement(5525..6475)
                     /locus_tag="PKH10_00550"
     CDS             complement(5525..6475)
                     /locus_tag="PKH10_00550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT26640.1"
                     /translation="MPSVSVLMPVYNGERYLAAAMESILAQTFGDIEFLIVDDGSADA
                     TPAILAGYAARDARVRVLRNPSNIGLVASLNRGLEEARAPLVARMDADDIARPERLAR
                     QVAFLRDNPDHGLVGTWAEIWVEDEKSPRAHRHPASNALLRTLLLFNNPFVHSSVMFR
                     ADMARELGGYAVGRTGSFTEDYEFWSRIARVRKVANIPLFLQVYREQAGSVTDRRENR
                     YADSIVEISVANIEALIGDGHGRFIRDIVRMYHKQFGPYDTNALDTIHFAVTLLTKAW
                     RTYGLDPRGIPLYGKMFAGILKNYCQNRRFVRAHENTSHG"
     gene            complement(6484..7434)
                     /locus_tag="PKH10_00555"
     CDS             complement(6484..7434)
                     /locus_tag="PKH10_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT26641.1"
                     /translation="MTPYARKRVLVTGGAGFIGSALCEKLLGMGHDVICLDNFFTGSK
                     NNIVHLLPDPFFEIVRHDITAPIFLEVDEIYNLACPASPVHYQYDPVATVKTSVMGSI
                     NILEMAKRGRMRTLQASTSEVYGDPEIHPQTENYRGNVNPIGPRSCYDEGKRCAETLF
                     FDYFRQYHIPIKVARIFNTYGPRMSENDGRVVSNFIVQALKNEDITIYGDGRQTRSFC
                     YVDDMVTGLIRLMESDGDFTGPVNLGNPGEIAMIDLAQRIIALTGSTSRVCFKPLPQD
                     DPVRRRPSITLAEDRLGWRPTVPLEEGLKRTIDYFRGVLG"
     gene            complement(7431..8270)
                     /locus_tag="PKH10_00560"
     CDS             complement(7431..8270)
                     /locus_tag="PKH10_00560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT26642.1"
                     /translation="MADKVSVIIPTWNRGYCIGRTVRSVLSQTYDDMEVLVCDDGSTD
                     DTADIVRRIADPRVKWLPGPRGGRPAIPRNRGIRAASGEWIAFLDSDDIWLPNKLMMQ
                     MSLLDRTKTKACSANALCLLPNGHISGAYLSREKEILTFEELVETNWVICSSALIHRS
                     LFDRTGFFPECPAMKAIEDYAFWLRIASCADISYCSAPLLYYLDAPAQSIRREASTDT
                     DMQRRLIFADFQEWAEEGLRQDDAEAKMRMLRATWFVLPGVRNVRKKKTIRDMFGMFR
                     RTR"
     gene            complement(8291..8938)
                     /locus_tag="PKH10_00565"
     CDS             complement(8291..8938)
                     /locus_tag="PKH10_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26643.1"
                     /translation="MKLHLGCGETYLDGYVNIDFPLEAHSVQRTTKADLCVDLLSLRY
                     PANSIEEVRLRHVFEHFTRPVACALLAGWHRWLVPEGVLRLEVPDLERTAKKINSRFV
                     PMARKLVGIRHLFGSHEAGWAAHYEGYTSATLSHLLKHFGFAVRSVTYNDWQGTYNIE
                     VVAFKRIDVVDVNPEERARNYLRHYLLDESEVALLEVWMVAWHRQHERMSGDDEK"
     gene            complement(8946..9749)
                     /locus_tag="PKH10_00570"
     CDS             complement(8946..9749)
                     /locus_tag="PKH10_00570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT26644.1"
                     /translation="MKVSIVIGSYDRKRFLKLAIESVREECRDIEHEIIVVDGGSTDG
                     SLKWLCAQKDIIAILQHNRGEWEGRPVRRRSWGYFMNLGFKCAQGKYICMLSDDCLVV
                     PGAIRNGIELFERSLAEGRRVGAVAFYWRNWPEMKKYWVGLTLGRRMFVNHGLYLNDA
                     MKDVGYCDEETLSFYHGDGDLSLKMWHAGREIIAGEDSYVEHYSHANMRARASNLALQ
                     KDDWERYLRKWTGIFYDPAKGDIGDWIEKEMGPGSGIEKRFERMGLLWW"
     gene            complement(9746..10975)
                     /locus_tag="PKH10_00575"
     CDS             complement(9746..10975)
                     /locus_tag="PKH10_00575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT26645.1"
                     /translation="MKVLHISTGDSGGAGLCCIRLHKGLLANGRESKVLVLNKTLNEP
                     ELYRFEQKSEKPPFPISFVWPYLALTARKFGIPLSRVERYRDRLNKLREGRQVFFTLP
                     VSDLDLAGHPLVRETDIIHLHWVADFLDYPSFFAKVNKPIVWTVHDENLYMGGWHYRR
                     AREEYPLLDELDDELASIKQRSLARQRKVTIVSLSRMMREFAVSHRSFTDREHRVIHN
                     PVDHMTFRPVDKFFSRELLGLPKDKKILCFVSFGLWDTRKGLKELLVALKSLRRPDIV
                     LCAVGAGDVKGISDQETFSLGLVRDERLMAAAYSAADLFVMPSFQEAFAQAPLEAMAC
                     GVPVVAFPCSGTEELINERNGIRTVDFTVESLREGIAKALNTKYDPEWIRNDVIERFG
                     IEKIVSQYVDVYQQVLT"
     gene            complement(10978..11616)
                     /locus_tag="PKH10_00580"
     CDS             complement(10978..11616)
                     /locus_tag="PKH10_00580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT26646.1"
                     /translation="MSYYDKDYFAWQKDLGKFGGVANLFKFREHVLPNHRVLDFGCGG
                     GYLLKNLPCAARYGVEINPAARSAAEENGVKTFATSGEVPDETVDVLISNHALEHVFN
                     PHAEIVELRKKLILGGVAVFVVPHNSYPEEWMPNDVHKHLYTWNRLTLGNLFSVAGFA
                     VDCVELIQHQWPSGFEELFKRHGEAKFHELCRREAKKNNIYQVKIVARREGD"
     gene            complement(11627..12580)
                     /locus_tag="PKH10_00585"
     CDS             complement(11627..12580)
                     /locus_tag="PKH10_00585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT26647.1"
                     /translation="MSMLAPVVLFAYNRPEHAERTITALLRNGEASQSDVFIYSDGPK
                     TGDEEAVAKVRGYLKTVQGFNSVTVIERDENRGLADSIVAGVTEVVESYGRVIVLEDD
                     IVTSPFFLRYMNDALRIYEHDERVMHVSGFLPRSGFSWLLPPTFFLRFMSCWGWATWR
                     RAWAKFNPDAKALYDELLKRGAIHDYNLDGVLSFHGQLEENIQGKIRTWATKWFTTIY
                     LEEGLCLYPRESLVQNIGFDGTGVHCGVAGTVNPYEPPLTGNRIPVRRIPFRQSRIGR
                     FYLKRFYRNLPREPLSAGIMRRALKIRQLAVRSYRWILEKQ"
     gene            complement(12624..>14025)
                     /locus_tag="PKH10_00590"
     CDS             complement(12624..>14025)
                     /locus_tag="PKH10_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNT26648.1"
                     /translation="MYLHYQYVPEPSTLLQGVRKLPAAHTLLLSHDDWNAEPVRYWDV
                     RSAETPEYASAQPRDIPDIIRARLEESVALTLRSDVPVGLALSGGIDSGAIAAFARRH
                     TADQFHAFGIGYPGRPPYDEREQAKGLAHKLGITFHEVELKVDDFVAFLPTLVSIMDE
                     PIADIAAFGHYAVPRAAAEHGIKVLLSGTGGDEVFWGYPWVVRSIEINEFLLRHPFVS
                     RLRHLPKGLWRYEPLWKVCSHSRLLNAYSTILREISVIDMRKPPEQLFFYEMVKDFVN
                     ARSLRTRVYGPAMRDISADNVFLPTALTALSEADIPATTLRLLFDTYLTSNCIALGDR
                     VSMGCGVEVRLPFLDARLIESVMALRRRHPDHQRGHKAWLRAALKEVLPDEVLTRPKS
                     GFQPPIMAWLDGARERYGALLREGLLVNSGILDMSRLESFLKESGPNDPPKQFFLYKL
                     ILLEIWYRSVVRRERF"
BASE COUNT         3228 a         4207 c         3587 g         3003 t
ORIGIN      
        1 cagtggaatc gatgtatcgt cactgttcca tcgtatcgac accgggtccc tccgggtcgg
       61 gagtcaggac gaccaccggc accgcgccat cctgcggcag catgtcggcg ataaagcgtc
      121 cgcgataatc atccggcgat aacgggaacg agtgcgagat gatgagcgtc ggtttctcga
      181 acatgcgcct accggctgaa aaatgctgtc aatatgcgac gcaagaaaac gaccggcgaa
      241 cggtcatcca gcgaacgggc cgcgcggtca caggccatca cgagagccgc ctcgtcaaaa
      301 aaacaccatt ccgggctctt ctcccgtacc tgatccagcc accggatggc ttgccgacgg
      361 tcctccggcg agatcgtcgt gaaccgggca tcgtaaaagc gcgcatgcgc atcgagttgc
      421 gcctcatcgg gcatgaaccc catcgtcttt tcgatgaact gcccccatat ccgcttgagc
      481 acctctctcc ggcgcatcga gcggccacga tccgacagca cccttccggt aacgctcccc
      541 agaccgacac ggtaacggag caacacttcg gggatgaccg ccagttccgt cacgagcgcc
      601 atctcgcacc acaggcggta atcctcggcc gtctcgtaac cctcacggta acgcaggcca
      661 tgttccgcca acaccgaccg gcgcatcatc accgaggggt gtacgaaggg ggtcgcgaaa
      721 agcagtcccg ccttcagcgc gcccgaattc cggggagcct tctgtgtcca ccgggggccg
      781 aacgggatga acttttgcgc ccacgagccg cacagaccga cctcgggatg cgcttccatg
      841 aacgaaacct gttcctccaa ccgtcgcggc accgagatgt cgtcggcatc catccgggcg
      901 aggtaacgac catgcgcctc gtcgatgccg cggttgagtg aaaagataag accttggtta
      961 cgatcattcc ggagcaggcg gatccgcgga tcgtcaactt tccctatctc ttctgccgta
     1021 ccgtcgcttg agccatcatc gacgaccaca agttcaaagt tgctgaacga ctgcgtcagg
     1081 atactacgga tcgccacccc gatatgtcgc tcggcattat aggcagggag aatgaccgac
     1141 acaagcggat cgctcatatc cgcacccacc ctcccggtat cagatcgccg gtctgccgtt
     1201 cagccaactc attcgcgaac catcttttag gagaaacaat tattttctcg gggttgtggt
     1261 taagccacgc accccaccag gaaaaagaac tgttcgctat gatctgatgt ttgcaggaac
     1321 tcatgagata catgtcatgg accgccgagc cggtggacac attacgcacc gggcgatcga
     1381 aacggaggtt ttcctgaacc catggcatgt cgtccgagaa gatatatatg tcttcgatcg
     1441 acacctttgt ggcgacgaaa tctactgctt tttcataata tgcgccaccg agcagtccgt
     1501 gaaaggcacg cgttgtcggg ttgtgcgcat agtcgccgcg gcgaacatgg agcgccagcg
     1561 aggcgcggga ggacatttct ttttctaaaa gagcatcttc ctccgataac ggttcgcgcg
     1621 gaacgaactc cgccagcaat gtctccctga tgtccgagaa gtatctctcc gactggaaat
     1681 agccccgtag cgaaaccggg tcggtcagcg agtggaagga ttgatcgaac tggagatcgc
     1741 gctcagcgaa gacatgtgtg cttccggtga tgcggcaccg tagccgcccc cacaggcccg
     1801 gctgtcccca cgcggctatt atttggtaac gagacagatc gaaggggcgg ggggtatcct
     1861 tcgacagttc cgagaaccag ctcagatcga ggaagagggg ctgctccagt cgcagggaaa
     1921 gcgcacgccc cgccgcatac tgaaacatct ggttgcccag accgccgcag agacgcaccg
     1981 tgatcatcgc gccgacctct ccgtccggta tcgttcctga agttgccgta ccctctcata
     2041 ctgcgaccgg taccaatcgg ccggaatctt gccatcgtag ccgcccggtt tgaacgaggc
     2101 gcttgcagcg atcatctctt caagaatcgc agcgaaagac gcatcgtcgg aggaaagact
     2161 ttcgacccac ttttcgtaga ggttccccac ctcaaagact tccctgcgtt ctcgagaaag
     2221 agctcgggta cttaatgcaa aatggaagag caggcctcca atattctgtc cgaaaacacc
     2281 atcatgcggc gtaagaccaa taaagctagt aattggtatg tttattttag gaccgagtct
     2341 attgatatcc ataaaatgat tggccgccac tccgttatcg caccccttac gcaggtaggg
     2401 ataacgatcc tcggctaaat tctgcagaat gggacatttc ttttgaaata aggcccggta
     2461 aatgttctta aaggaaaatc catgcctttc aaacaaggca actaagtctc tgaaataatg
     2521 ctcaacagtt ctcgactcgc tattggcacg ggtaaatcct atgccttccg aaacaacacg
     2581 cgcatctgcc gtccgcatga cacgatacag attgttggta caagagaacc attcgttgtc
     2641 gtacaatgca aagcccggca tgtagcccga ttcgggttgc cataaggggg agccccaagc
     2701 gtcttgataa taactatttg agagccccgt atcgagtccg acagaaacac tgcgatattt
     2761 ttctaaaagc catatcggaa aggcaatcca atctacagat aaaggcaaag tgtcagcatc
     2821 aagcattact gaaaattgtt ccgagaccag cttcaaccct tcgtcgagaa gtaatcctat
     2881 atctgcggcg ttctttttgt tgtggcgccg tatctgataa aactctataa aaggaaatcg
     2941 cgcgaattct ttacgagcca gatcaaagct tccatcctgg ctttcgtcat caagcacata
     3001 aatcttctct ttcaacagcg gatcgcgatt cttaacgata tgggtaaggc acggtttcag
     3061 aaaacgataa ctttcccaat gcgggatcag caccgccact gaacgaggcg cgtcaatggc
     3121 atccagcgtt atagccaaaa gtcttgtatg cctattcttc gtatcatgca tgtgtttata
     3181 tttcctgacg actgatgaat cggcacgaag agcattaaaa ggcaacaagg ggatatggca
     3241 ccccaagaaa tcctccaata cctgcaaatc gatcacgggc aagcccgact gatcactcac
     3301 aggcacgacg accacgactc gatgccgagc gattcgcatc attttcttaa ggtcttctac
     3361 tagatcggcg accatccttc tcttggaaag cagtgtcagg taatcagatc cgccctcatc
     3421 cccgcgatca cccgaagttg gagaaaatga tgtattaccg gcagatggag ggactttcga
     3481 agaaaaatag gggcctgccc atacctcctg ccaagacttc tgtgatatct tatttttctt
     3541 gttccagtcc cgccaaagag gcagagacag ttctccttca gaagcccatc ggatgtcagt
     3601 tacttggaaa ttttgcatct tcctcgcccg caccgtcttt ttttgttatt aaaacagcca
     3661 tctgttgcgg gatttcccct cgtgaagaat aggtccatcc cgcagtctta aaaacgaacc
     3721 aatccaataa gcaataacga agctgaaagc ctatttcatc aaaacagaag acatgcccca
     3781 agcccccctc aacttcatat ccatcggtga ccggttgctt gtaaggggcc aacactatca
     3841 attgttttga aaatgacaac aaaacatcgc aaacattcca aggatcaaga aaatgttcaa
     3901 gaacattgga agcaattgcg agttcatatc tcatatcagg agacaatggc gtttccagaa
     3961 tatttctcat catgaacagc gtccttttgt tggaatatct tgcatttgca taatcaatag
     4021 cattttgaga tatatcgact cccaaatatt ccgccttagt gtactttctt aacatttttg
     4081 agagatctcc tgtcccgcaa ccgaactcaa agatcgatct ggactgctca atcgctctac
     4141 ggaaaggaac atgcctacca acaccccata atggcgcgcg aatttcagaa aagaaatctc
     4201 gagaaatctg caggttttgc atggagtggt tttgggccgc ttttgcgaaa gcctgatccc
     4261 aactattaac tgcgcgtaaa aagttcgtca tgacttgctt gtaataagcg tttgatataa
     4321 ttgaaaatat cttgttgcca cagattccca agtgaatcgt tcgcgccacg cggcacgccc
     4381 tgcatcggcc attcttctcc tccggtcggc atcacgccat aattcctcga gcatcgcggc
     4441 ggaaccgcca atgtcggcct gcgagtagcc cttccggtca tgggatgtcg gcagtatcgc
     4501 tccgccgccg gtccacgccg cgatctcggc ggcgttgccc acatcggtgg caaggaacgg
     4561 cgtcctcgat gccgccgcct cgaacagtac tatcggagaa cactcgacgc gcgaagggaa
     4621 caggaacagg tcggcctctc ggtaggccat caccgtcgcg caccgctcca aatcggcgaa
     4681 cagcaggcgc ttcccggcgg agcgccagtg ctccattccg ttgaagtttc gctcggcggc
     4741 gcggcagaac cgtgggcact cgtcctgcgc cctccggaga aggcgccgcg cgacgtcgcg
     4801 cgcccatgtc cggaccgaga aggcgggagc gacggtgtcg cagtagttcc ccacgatcaa
     4861 caggacggcg ttcgagacct gtgcggcgtc gaatatcctg actgcctcgc ggtgtccttt
     4921 cataccggta tgcgaaccga ccagaagcac caggaacgca tcggcgggta tccccagccg
     4981 ttcccgtagg gagaaggcag cctcagcctc gaactcctcc tcggaggcgc cgttgggtat
     5041 cagcactcgg ttctcgatgc cgtgctcctc ggcaaagcgg gcatcgcggt aggtgtccga
     5101 cagaaagaca ttcatgtcgt acttccgcat ccagccgggc atcttttcga aatagtcgcg
     5161 ataacttccg agaaagagac ctgagaagcc tgtcggcaca agtaccttac gcgcctttat
     5221 ctcgtcgagt atcggcaagg tgagatcggt cgcccactgt tgcgccgcaa aattcgtgac
     5281 tatatcaaag tcaccttcac ggagaagatg ttgatagcgt tccatttctg ccaggtcgcc
     5341 gccatacccg ttcaccatgt tgcccgaaat ggcgaaacct tctaccctaa cgccgttgat
     5401 aatcttctca acgcgttcgg gattcgtaga ggttgcgacc gtcacctcat gtcccatgcg
     5461 gacgagccgt tccgatatct gcgaaacgac ttcctgcata ccatgccggg aaggcaggta
     5521 agactcaacc gtgtgaagta ttttcatgag ccctaacaaa acgacggttc tgacaataat
     5581 tcttcaagat accggcaaac atctttccgt acaaaggaat accccgcgga tcgaggccgt
     5641 aggtgcgcca cgccttcgtc agcagcgtga ccgcgaaatg gatcgtatcc agcgcattcg
     5701 tatcataggg accgaactgc ttgtggtaca tccggacgat atcacggatg aaacgcccat
     5761 gcccatcccc gatcagcgcc tcgatgttcg cgacacttat ctcgacgatc gaatctgcat
     5821 accggttctc gcggcggtcg gtcacgctgc cggcctgttc gcggtagacc tgcaggaaga
     5881 gcgggatgtt cgcgaccttc cggacccgcg cgatgcggct ccagaattcg tagtcctcgg
     5941 tgaaactccc ggtcctcccg acggcgtacc cgcccagttc gcgtgccatg tcggcacgga
     6001 acatgaccga gctgtggacg aagggattgt tgaacagcag cagcgtccgc agcagggcgt
     6061 tcgatgcagg gtgtcggtgc gcgcggggcg atttttcatc ttccacccat atctcggccc
     6121 atgtccccac gagaccgtga tctgggttgt ccctcaggaa ggcgacctgc cgcgccagcc
     6181 gttcggggcg agcgatgtca tcagcgtcca tgcgcgccac cagcggggcg cgggcctctt
     6241 cgagaccgcg attcagggag gcgaccaatc ctatgttcga aggattacgg agcacccgca
     6301 cccgggcatc gcgtgccgcg tagccggcca gtatcgccgg tgtcgcatcg gcagaaccgt
     6361 cgtccacgat aaggaactcg atatcgccga aggtctgtgc gaggatgctc tccatcgccg
     6421 ccgcgaggta gcgctcgccg ttgtacaccg gcatcaggac gctgacggag ggcatcccct
     6481 ctctcatcca agcacgcccc taaagtagtc tatcgtgcgc ttcagcccct cctccaacgg
     6541 gacggtcggc cgccacccga gacgatcctc ggccaatgtg atggagggcc tccggcgcac
     6601 cggatcgtcc tgcggcagcg gcttgaagca taccctgctg gttgagccgg tgagcgcgat
     6661 gatccgttgc gcgaggtcga tcatcgcgat ctcgccgggg ttccccagat tgaccggccc
     6721 ggtgaaatcg ccgtcacttt ccatcaaccg tatcaggccg gtgaccatgt cgtccacata
     6781 gcagaaactc ctcgtctgcc ggccgtcgcc gtagatggtg atatcctcgt tcttgagcgc
     6841 ctgcacgatg aagttgctca ccacgcggcc gtcgttctcg ctcatgcggg gaccataggt
     6901 gttgaatatg cgggcgacct tgatggggat gtggtactgc cggaagtaat cgaagaagag
     6961 cgtctcggcg caccgcttcc cctcgtcgta gcaggaacgg ggccctatcg gattcacatt
     7021 cccgcggtag ttttcggtct gcgggtggat ctccgggtcg ccgtacacct ccgaggtcga
     7081 agcctgcagc gtccgcatcc tgcccctttt tgccatctcc agaatgttga tggatcccat
     7141 caccgaggtc ttgaccgtgg cgactggatc gtactgatag tgtaccgggc ttgccggaca
     7201 agcgaggttg tatatctcgt cgacctcgag gaagatgggg gcggtgatgt cgtgtcgcac
     7261 gatctcgaaa aagggatcgg gcagaagatg gactatgttg ttctttgatc cggtaaaaaa
     7321 attgtcaagg cagatgacat catgccccat gcccaacagt ttttcgcaca gcgccgaacc
     7381 gatgaaaccg gcgccgccgg tgaccaatac tcgttttctc gcgtaggggg tcatcttgtc
     7441 ctcctgaaca ttccgaacat atccctgatg gtcttcttct ttcgaacatt tcgcactccc
     7501 gggagaacga accatgttgc cctcaacatc cgcatcttgg cctcagcatc gtcctgtcgg
     7561 agtccctctt cggcccactc ctgaaaatcg gcgaagatca atcgacgctg catatcagta
     7621 tcggtcgatg cctcccgacg gatcgattgt gctggagcat caagataata caacagggga
     7681 gcagagcagt aagaaatatc cgcgcacgaa gctatgcgca gccaaaaggc gtagtcctcg
     7741 atcgctttca ttgccgggca ctccggaaag aaccctgtcc ggtcgaaaag agagcggtgt
     7801 atcaaggcgg aactgcaaat cacccaattc gtttcgacaa gctcctcgaa tgtaagaatc
     7861 tctttttctc gcgacagata agctcctgat atgtgaccgt tcggcaataa acataaggca
     7921 tttgctgaac acgcctttgt tttcgtgcga tcaagcaagg acatctgcat catcaattta
     7981 ttaggcaacc aaatatcatc gctatcaaga aacgcgatcc attcgccgga cgccgctcgg
     8041 ataccccgat tccgcggaat agcgggcctt ccgccgcgag gaccgggcaa ccatttcacg
     8101 cggggatctg cgatccgtcg tacgatatcg gccgtatcgt cggtcgaacc atcatcacag
     8161 acaagcacct ccatgtcatc atatgtctgt gaaagcacac tgcggacggt ccgtccaatg
     8221 caataccccc ggttccatgt ggggatgatg accgagacct tatcggccat ggaacctcct
     8281 tgagacggcc tcacttttca tcatcgcccg acattcgttc atgttggcgg tgccaagcaa
     8341 ccatccagac ctccagcaac gccacttcgc tctcatcgag aagatagtgc cgcaaataat
     8401 tccgtgcccg ctcctcagga ttgacatcga ccacatcgat ccttttgaat gcgacaactt
     8461 cgatattata ggtcccctgc cagtcgttat aagtcaccga tcggacggca aacccgaaat
     8521 gcttcagaag gtggctgagc gtcgcggagg tatatccctc gtagtgggca gcccaccccg
     8581 cctcgtgtga tccaaagagg tgacggatcc ccaccagttt tcgtgccatc ggaacgaatc
     8641 gggagtttat tttcttcgcg gtcctctcta ggtcagggac ctccaaacgc aacacgccct
     8701 ccgggacgag ccaccgatgc caacccgcga gaagagcgca tgcgacgggt cgggtgaagt
     8761 gctcaaagac atggcgcaac cgcacctctt cgatgctgtt ggcgggataa cgcaatgaaa
     8821 gcaggtcaac gcataggtcg gctttcgtcg tcctctggac cgaatgggcc tcgagcggaa
     8881 agtctatgtt gacatacccg tccagatatg tctcgccgca ccccagatgc agtttcatgc
     8941 cttcctcacc accataacaa ccccattctt tcaaacctct tctctatgcc gctccccggc
     9001 cccatctcct tctcgatcca atcgccgatg tcccccttcg ccggatcgta gaaaataccg
     9061 gtccacttcc tgaggtaccg ttcccagtca tccttctgca acgcgaggtt cgatgccctc
     9121 gcacgcatgt ttgcatgcga gtagtgctcc acataggagt cctctccggc gatgatctcg
     9181 cgaccggcgt gccacatttt tagactgaga tctccgtcgc catggtagaa cgaaagggtc
     9241 tcctcatcgc agtagccgac atctttcatc gcatcgttca agtataatcc atggttgaca
     9301 aacatcctcc gccccagagt cagtcctacc cagtacttct tcatctcagg ccagttcctc
     9361 cagtagaagg cgacagcacc aaccctccgt ccttccgcga gcgatcgctc gaagagttcg
     9421 ataccgttcc gtatggcgcc gggtaccacc agacagtcgt cgctcaacat acagatgtac
     9481 tttccttgtg cacacttgaa gccgaggttc atgaagtaac cccacgaacg ccgtcgtacc
     9541 ggacgcccct cccactcccc cctgttgtgc tgcaggatgg cgatgatgtc cttctgcgcg
     9601 cagagccact tgagcgaccc gtcggtcgag ccaccgtcaa ccacgatgat ctcatgctcg
     9661 atatcgcggc actcctcgcg gacgctctct atcgccaact tcaagaatcg cttgcggtcg
     9721 taactgccga tgacgatcga aaccttcatg tcagcacctg ttgatataca tccacatact
     9781 gcgatacgat cttctcgata ccgaagcgct cgatgacgtc gttacgtatc cattcagggt
     9841 catacttggt attcaacgct ttcgctatcc cctcccggag cgactcgacg gtgaaatcga
     9901 ccgtgcggat accgttcctt tcatttatca gttcctcggt cccgctgcac gggaaggcga
     9961 ccaccggtac gccgcaggcc atcgcctcta aaggagcctg agcaaaagcc tcctgaaaag
    10021 aaggcatcac gaacaagtcg gcggccgaat acgccgccgc cattaaacgt tcatcacgca
    10081 ctagccccag agaaaatgtc tcttgatcag atattccttt tacatcgccg gcaccgaccg
    10141 cacatagcac gatgtccgga cgcctcaaac tcttcaaggc caccaacaat tccttgagtc
    10201 ccttgcgagt atcccagagg ccgaacgaca caaaacataa tatcttttta tccttgggca
    10261 gacccaacag ttctctcgag aagaatttgt cgaccggacg aaaggtcatg tgatcgaccg
    10321 ggttgtgtat cactcggtgt tctcgatccg tgaaagatct atgggaaacg gcgaattctc
    10381 tcatcatgcg cgacagggaa acgatcgtga ccttcctctg gcgcgccaaa cttctctgct
    10441 tgatacttgc caactcatca tccaattcat caagcagagg atactcctct cgcgctcttc
    10501 gataatgcca accacccatg taaagattct cgtcatgaac ggtccatact atcggcttgt
    10561 tcactttggc aaaaaaactg ggataatcaa gaaagtccgc cacccaatgc agatgaatga
    10621 tatccgtctc ccgcacaagc ggatgcccag cgagatcaag atcggaaacc ggaagggtga
    10681 aaaagacctg gcgcccctct cgtagtttat taaggcggtc acggtagcgt tcgacccgag
    10741 acaacgggat cccgaatttg cgcgccgtca atgcgagata gggccacaca aaagatatcg
    10801 ggaagggcgg cttttcgctt ttttgttcaa aacgatataa ctctggctca tttaatgtct
    10861 tattcaggac aagcaccttg ctttctctgc cgttcgccaa gagcccctta tgcaaccgga
    10921 tacagcaaag tccggcccct cccgaatcac cggttgaaat gtgcagtacc ttcatcccta
    10981 gtcgccctct cttcttgcga cgatcttcac ctgatagata ttgttctttt tcgcctctct
    11041 gcggcataac tcatggaatt tcgcctcgcc atgcctcttg aacaactcct cgaaaccgga
    11101 aggccactgg tgctgtatca gttcaacgca gtccaccgca aaaccggcga cggaaaagag
    11161 gttccccagc gttaggcggt tccatgtgta gagatgtttg tggacatcgt tcggcatcca
    11221 ttcctcggga taggagttgt gcggcaccac aaagacagcg acacctccca agatcaactt
    11281 ctttctgagc tccactattt cggcatgtgg gttgaaaaca tgctccaacg catgattgga
    11341 gatgagcaca tcgacggttt cgtccgggac ctcgccggac gtggcgaagg tcttgacgcc
    11401 gttctcctcc gcggccgaac gcgcggcggg attgatctcc acaccgtacc gggcggcaca
    11461 cggcaggttc ttcagaagat aacccccacc gcagccgaaa tcgagtaccc gatggttcgg
    11521 cagaacatgc tccctgaact tgaacaggtt ggcgacgccg ccgaacttcc ccagatcttt
    11581 ctgccatgcg aaatagtcct tatcgtagta actcaccggc ccctccttac tgtttttcca
    11641 atatccatct atacgatctg accgccagtt gccgtatctt caacgcccga cgcattatcc
    11701 ctgccgacag cggctcgcgc ggcaggtttc tatagaatct cttcagatag aatcgcccta
    11761 tccgcgactg cctaaacggt atgcggcgca ccggtattct gttccccgtc agcggcggct
    11821 catagggatt aaccgtaccg gcaacgccgc aatggacccc cgttccgtcg aaaccgatgt
    11881 tctgcaccag cgattcccgt ggatagagac agagcccctc ctccagatag atcgtggtga
    11941 accacttggt cgcccatgtc cttatctttc cttggatatt ctcctccaac tggccatgga
    12001 aagaaaggac accgtccaga ttgtagtcat ggatcgcgcc ccttttcagg agttcgtcat
    12061 acagcgcctt tgcatcgggg ttgaacttcg cccacgcgcg gcgccaggtc gcccagcccc
    12121 agcaactcat gaagcggagg aagaaggtcg gcggcaaaag ccacgagaag cccgatcgcg
    12181 gcagaaaacc gctcacatgc atgacccgtt cgtcatgctc ataaatccgc aaagcatcgt
    12241 tcatatagcg aaggaaaaat ggggatgtca cgatgtcgtc ctccaacacg atgacccggc
    12301 catagctctc gaccacctcg gtgacgccgg cgacgatgga atcggccagc ccccgattct
    12361 cgtcgcgctc gatgaccgtc accgagttga atccctgaac ggtcttcagg tacccgcgga
    12421 ccttcgcgac cgcctcctcg tcacccgtct tcgggccatc ggaataaatg aagacatcgc
    12481 tctgcgaagc ttcgccgttg cgaagcagcg ccgttatcgt cctctcggca tgctcaggcc
    12541 gattgtaggc aaaaagaact acgggggcaa gcattgacat cattactcct tcttttaacg
    12601 acttgacggc tggccacaca aaatcaaaat cgttctctcc gcaccaccga ccgataccat
    12661 atctccagta agatcagttt atacaaaaaa aactgtttcg gtgggtcatt cggcccgctc
    12721 tctttcaaga aagattcaag ccgcgacatg tcaagtatcc cggaattaac gagaagtcct
    12781 tcgcgcaaca aggccccgta tcgttccctc gccccatcaa gccatgccat tatcggtggc
    12841 tggaatcccg acttgggacg ggtcagcacc tcatccggta acacctcctt gagcgccgcg
    12901 cgaagccacg ccttgtgacc ccgttgatgg tcgggatgcc tccggcgcag cgccatgacg
    12961 ctctcgatga gccgcgcatc aagaaaggga aggcgcacct cgacgccgca ccccatgctg
    13021 acccggtcgc ccaaggcgat gcagttcgag gtcaggtagg tgtcgaaaag gagtcgcagg
    13081 gtcgtcgcgg ggatatcagc ctcggaaaga gccgtcagcg ccgtaggaag aaagacattg
    13141 tcagcggaga tatcgcgcat ggcggggccg tacacccggg tacgcaatga ccgcgcattg
    13201 acaaagtcct tgaccatctc gtagaagaac aactgttcgg gcggtttccg catgtcgatg
    13261 acggaaattt cccggaggat cgtcgagtag gcattgagca gtcgggaatg gctgcagacc
    13321 ttccacagcg gctcatatct ccacagtcct ttgggcaggt gccgcagccg gctcacgaag
    13381 ggatggcgga ggaggaactc gtttatctcg attgagcgaa cgacccacgg gtagccccag
    13441 aagacctcgt cgccgccggt tcccgaaaga agcaccttta tcccgtgctc ggcggccgcc
    13501 cgtgggactg cataatgccc gaaggcggcg atatcggcga tcggctcgtc catgatcgaa
    13561 acgagcgtcg ggaggaaagc gacaaaatca tccaccttga gttcgacctc gtggaaggtg
    13621 atgcccagtt tgtgcgcgag gcccttcgcc tgctcgcgct cgtcgtaggg cgggcgcccc
    13681 gggtagccga tgccgaaagc gtggaactgg tccgccgtat gccttctggc gaaggcggcg
    13741 atcgcccccg aatcgatgcc tcccgaaagg gccagtccga cagggacatc ggatcgtagc
    13801 gtcaacgcga cagactcctc gaggcgggcc cggatgatgt cggggatgtc ccgcggctgc
    13861 gcggaggcat actccggcgt ctccgccgac ctgacatccc agtagcggac cggctcggcg
    13921 ttccaatcgt catgcgacag aaggagggta tgcgccgccg gcagtttgcg gaccccctgc
    13981 aacaacgtag agggctccgg gacatactga tagtgcaggt acatg
//
LOCUS       DAOQUE010000009        14022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_4918_length_14022_cov_4.045250, whole genome shotgun sequence.
ACCESSION   DAOQUE010000009 DAOQUE010000000
VERSION     DAOQUE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14022
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4918_length_14022_cov_4.045250"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKH10_00595"
     CDS             complement(<1..623)
                     /locus_tag="PKH10_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HNT26649.1"
                     /translation="MSRFRELIASAPGSRHILQGNEAFALGVVHAGYHAADGYPGTPS
                     TEVIDKYLSQVQDKLVVGWSVSEAVATAVTVGHAVAGSDAVVTMKIPGVFQAGDALTT
                     SAFYTAQMGAFIIYAATDYVPSSTQHVIDARHFFSAARVPVLEPRDHQEMYDIAFIAA
                     DMSRRFRTQVAILASGILAHSEGLVTTRAPRTIEKRPLSENLHGWML"
     gene            complement(704..1513)
                     /locus_tag="PKH10_00600"
     CDS             complement(704..1513)
                     /locus_tag="PKH10_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT26650.1"
                     /translation="MDIPNFLPGDAYDTPDNIARPIGDRLLLGSRWYFYLMMLDIQRR
                     YSAIARTGGYDRAVWARSSIEAFRLLESVGCRFRLKGLDHLRRDPAEPLVIASNHMST
                     LETMVFPGIIGGLRPVTFVIKENILRNPLYRDIMAATRPIVVSRKNPRDDLKKVLEEG
                     AAHLREGTSVILFPQATRSDTFDPAQFNSLGVKLAKTAGVRLMPVAIKTDFWGNSRFR
                     LLKVVGPLHRERPVFMDFGAPLAITGNGTEEHHAVVEFIKTRLAEWDNAPR"
     gene            1611..2720
                     /gene="thiH"
                     /locus_tag="PKH10_00605"
     CDS             1611..2720
                     /gene="thiH"
                     /locus_tag="PKH10_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HNT26651.1"
                     /translation="MSERTFHIDEERTLRLRDRALAATAADIAAVLGQEHLSPDELAP
                     LLSPAMDDALFMRLAAESARLTRRRFGYALSLYIPLYYDNRCINRCRYCGFNAGNEVP
                     RVFLSREEILREGRAIRRHGFTNLLLVAGEHPESADPSFLVPVIRDLHDIGFRSVSIE
                     IAPLEEAAYRRLVTEAHLDGLYVYQETYHRATYAAEHPRGPKSDPDRRLATPERGARA
                     GIAKIGIGFLLGLYDWRYETLALAQHLSFLQKHHWRAEFSISFPRIRKAEGVAGGPHP
                     VGDREFLQMACALRLLFPETPMSLSTRESPAFRDAAVPIGFTNLSAGSSTVPGGYGTR
                     KGALAQFEVEDARGPAEVAAALRARGFDPVWKDWE"
     gene            complement(2745..4370)
                     /locus_tag="PKH10_00610"
     CDS             complement(2745..4370)
                     /locus_tag="PKH10_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT26652.1"
                     /translation="MSNRALILSLLAVLFFAAGGFLLLNLLFLDAYRMPAVSTQQFRV
                     AEMARDLEILHETLTNAESADPATVRPALYRLKEHYETIFGEIAGDLDPLLKNRFADY
                     HARIWPAMERWNATPGTGEVDPAQLRGDLRALRKLVLLAAKRLEKESLTERRGAILRS
                     ARFAAASFAVAIAIGLLGIIAAWRSRQRRDTITVPAEPPPDNPAGTPTPKEHRREQLL
                     LKRYRDILDSLPDGVVVSDASSGRVQFINRTFRDRFAVGQDQIGETVAALAEKSGLAI
                     AEPGKLTAHDTVYWRDITEVGGAVLHLVRDMTEQDRLSNRLMSAERLISIGEIASKVT
                     HEIRNPLSTIKLNVEYVADHAHDLSPEELSASIALIVKEVIRLEEITDRYMGMVRYRA
                     DEESPSRTPLRDAVAEVTRFHESEFRTRDIALTVGDLPAAELGIAFNSFREVLLNILK
                     NAWEELDRRGTVAISGDIADGKVRLFVDDSGRGVPAAEREKIFRNFYTTKPGGTGIGL
                     SHSLKLVTEAGGDIAVADSPLGGARFIITIPRG"
     gene            complement(4363..5526)
                     /locus_tag="PKH10_00615"
     CDS             complement(4363..5526)
                     /locus_tag="PKH10_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT26653.1"
                     /translation="MKNLLISLAFLFSLSLVAYEAPGSVEEVPVPPFIDVEKADFTQA
                     PDEHEVTSGDTLWDLSEKYLKSPWYWPKVWSINPQIKNPHLIYPGDKVYFRGTGEIMM
                     PPTNEGGLSIGGEGESSEDLETDETARTGKTLDDKPENYKNYVKLGGKYRVDRFTQIE
                     DTVLDVPYRGYVADETLKQAGKIMAAFDTKELLATEDSCYVQFTGKKHAVGDYLEFFR
                     VVEEITHPVTNDVVGNHIEITGKGKIIDINNEGIATVRIIKAYTAVQREDSVREFQER
                     RPNIKVEQLDNKKLQTIVLSGYDPVTMFGDGYLVYLDKGAKEGLVEGNMLTIHRRQDG
                     LDTLDDDDLAKLPLEPIGEVVVVLTEAHTSVAIITKSILGIERGDIATATIFE"
     gene            complement(5609..6205)
                     /locus_tag="PKH10_00620"
     CDS             complement(5609..6205)
                     /locus_tag="PKH10_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26654.1"
                     /translation="MKRVVVFSLCLLVAAACASTGTADPAKTPQDYRESALRYLPLAV
                     GNSWTYTVDYLGQTGEMTIRIEREENGWFIDNRGTALTADRHGIRDRDRYLLTFPLQE
                     KRAWVALTGPTRNETRSIVAIDDPVETPAGNFEGAVVVETTVNPQKGIFLKSLHYFVP
                     RIGIVKIETFVEDTGAGTKKRQTLTLLKSREIKNEPKN"
     gene            complement(6202..6873)
                     /gene="lipB"
                     /locus_tag="PKH10_00625"
     CDS             complement(6202..6873)
                     /gene="lipB"
                     /locus_tag="PKH10_00625"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNT26655.1"
                     /translation="MTLYWHLFDEPIEYGEALRLQERLRDEVLGGELGDGIVLFLQHR
                     PVYTLGFRGDERHILLPPEERERRGIALYRTRRGGDVTYHGPGQLVIYPIIDLRRARF
                     ASIRDFVVFWGTGLARTLSDRYGIPARWDETKTGVWVGRNKIAAVGVHVKKGVAAHGF
                     ALNIDPDPAHFAGIVPCGITDGGVTSIKRELGRSPRHEELARDLLSLLAERYGTDNLK
                     EYPLS"
     gene            complement(6873..7619)
                     /locus_tag="PKH10_00630"
     CDS             complement(6873..7619)
                     /locus_tag="PKH10_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNT26656.1"
                     /translation="MLIALISDIHGNLPALEAVIRRIVELRPDRLYFLGDAVGYGPWP
                     AESVEILRRNVHGAVLGNHDAGVVRKTDVSNYFDAIRYVIEWSREQLDDTQKDFLGKL
                     KYVLNDDSGLLLCSHGSPRQPEQFDYIYSKDTVEPLYEIEPFLRRVTFMAHSHLFSYF
                     VLTGEGRTIELDLSSGQIDIDFPYPSVCVIGSVGQPRDGDRRAGFVTFDTEKMTVMFH
                     RVEYAVERTVSQIRKLKLPRSFADRLLEGR"
     gene            complement(7621..8946)
                     /locus_tag="PKH10_00635"
     CDS             complement(7621..8946)
                     /locus_tag="PKH10_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNT26657.1"
                     /translation="MSKKIFVIDTNVILSDPKAIFHFEENDIVIPLVVIEELDKFKKD
                     MNQIGLAARSFARYIDSIRKRGSLLDGVQLKEGGSIRIVLASQVDLPNLDLGGSHADN
                     IILSSAYRVTKENPGVPVVLVSKDINMRIKADALGIQAETYKNETVNFTSLPKGFAPL
                     DVDGAIIDRIHQQKKLPLDDPATEPFRKGYSNQFFILKDLANEQHQSIVRIDPFDNCL
                     VPLKQYKSVWGISARNVGQKLALDLLLDDRVKLVSLMGMAGTGKTLLAIAAGLVKCLD
                     EGVYTRMLVTRPIYPMGRDIGYLPGTINEKLKPWMQPVFDNLEYLLAGYDSANLGKMN
                     DLMSSGVIEVEALTYIRGRSIPEQFIIVDEAQNLTPHEVKTIVSRAGENTKIVLTGDP
                     FQIDNPYMNESNNGLSYIVDKFKEFQLAGHVILEKGERSALANAAAELL"
     gene            complement(8974..10548)
                     /locus_tag="PKH10_00640"
     CDS             complement(8974..10548)
                     /locus_tag="PKH10_00640"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNT26658.1"
                     /translation="MTDPNIPRPTPVPESLSRELELKGKKDPFIGQTVSERYVVTSLI
                     GSGGMGNVYLAEHVLLKKKVALKILHFELSKNKDTVERFKREAIAASNIGQSNIVDVT
                     DFGYTEQGNAFFVMEYVEGESLADIIKKRAPLPLHHVIAISAQIALALYSAHSKGIVH
                     RDMKPENILITSKNEISPFVKIVDFGISKIIGTGVTSRKERTLTKAGAIFGTPEYMSP
                     EQAGGQQVDTRTDIYSLGVMMYEMSTGKLPFSDQNYMKVLHKHQYEIPDLPSSKEHGV
                     PPEMDAIIMRCLEKTAGNRYPTMLDLLADLKKVYGQHKLEKQINLAFLLNSTVILRAE
                     GEAPDTALPPPSEHDMLRMLNKETMAGRGRSDTVAPTRKKSVLVTAVIILALLLVAGI
                     LFYRAFIRPDRVLVAERTPQETQKPLPKQPDPVPEKPRPEPEKKIHQPSQTQKGGRSG
                     KRTILLKVNSNLAGVTVVNNETGKVLCTTPCKTRLPQDAGGVMVLGFQKDDFNIRPMA
                     IALDRDITVNVDLKQN"
     gene            complement(10613..11056)
                     /locus_tag="PKH10_00645"
     CDS             complement(10613..11056)
                     /locus_tag="PKH10_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26659.1"
                     /translation="MTPSEPRGDRPDRAARLLAVLLACAVALSIYLYFSLSRTRAELQ
                     TARQEQMKLMGDYKAALEDNHKLLTRSLSERYLDSLIDLRNRIEHGATVNDEEKKQAL
                     DRASFIVDNMGSLDLPPEEAKLRLQFLVTVKNLLAPPPPEGTEKK"
     gene            complement(11075..13633)
                     /locus_tag="PKH10_00650"
     CDS             complement(11075..13633)
                     /locus_tag="PKH10_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT26660.1"
                     /translation="MRRIIVSFLVVLFGSSLFAAKVYRSDGSFTEFAKDPALTARFAA
                     KADLTGAKAPAATWKLDRRYLVVDDSNAGTLPVYRFGDRLFVANDQIFWRGAMPVNAL
                     AKKYGLTLKEIYPSTRLYRFAVTGDAAELAQRIVEAGDGFAFPDMVREQVLFAEPVMP
                     ITDKYYTEGYQWALKNTGTAIGLSNPELPTKANADIRFEQAMQFLVDRIAANELDGFD
                     GATKVAIMDSGVDLTHPDLKNKTEEGWDAVNHEAGGDYGTIEDSMYGTAGYSHGTNCA
                     GVAAAEGNTIGTTGVCPWCGIYPVCFMEGGSGSASSEQGYIESYEKYVADEKIVAVNC
                     SFGPMAGMGEVPLMPGEEESHLNFLQNGRGGLGGAILYAAGNDGVDTNYMVLHGYEFK
                     FKRGDTDVVATILSIGGTSAWDNRVSYSNYGKEIAMVTPTLSSAPTLGIATTYIQTYG
                     DLDDDYSNRFSGTSAAAPFATGVMGVIFSVDPTLTLEEAVDIVKLSSDKINPETGFWD
                     KPLDAPAGDARSPKFGFGRANLLKAVRLAAGLDLCDAADIKAETANGLDDDCDGWVDE
                     GFALDLSTIGGPCTEKADCVTGDLTEADVDCLAAHGATKYDGGYCAVLPVKTDCPDGT
                     RAPGDGGAYCLKDCNDDHPCEREGFYCTEANLGFCTALCETNDDCTEESYCDPSGHCR
                     HDPSEPGGECETNEDCLYNGYCVTMVPGGFCYVSCEDGIDDYCGDQGAKCVHVNIPSM
                     GEADMCVPPCEEDADCRSMGGEQLKCHGLLNGKEDVCFMSCSSDEECFDENATCDNGT
                     CVNKSEVTDDIAATDDDLAGDDIAATDDLADGTVVPDTDTPAKKDDGCGCSLVF"
     gene            13801..>14022
                     /locus_tag="PKH10_00655"
     CDS             13801..>14022
                     /locus_tag="PKH10_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26661.1"
                     /translation="MRHAFFALLIGVAVLPFLSCTPVEEDPGTDTLAVTDSDAAVDPD
                     GTVDPDGTVNPDGTVNPDDPQGDGVVTDED"
BASE COUNT         2361 a         4241 c         4585 g         2835 t
ORIGIN      
        1 atcagcatcc aaccgtgcag gttctcggaa agcggtcgtt tctcgatcgt ccgcggcgcg
       61 cgggtcgtga ccaagccctc agagtgagcg agtatcccag acgcgaggat ggccacctgc
      121 gtccggaaac gccggctcat gtcggcggcg atgaaggcga tgtcgtacat ctcctgatgg
      181 tcgcgcggtt cgagcaccgg gacgcgggcc gccgagaaga aatggcgcgc gtcgatgaca
      241 tgctgggtgg aactcggcac atagtcggtc gcggcgtaga tgatgaaggc ccccatctgc
      301 gccgtgtaga aggccgaggt cgtcagcgcg tcgcccgcct gaaagacccc cggtatcttc
      361 atcgtgacca ccgcgtcgga gccggcgacg gcgtggccca ccgtcacggc ggtcgcgacc
      421 gcctcgctca ccgaccagcc gaccaccagt ttgtcctgta cctgcgaaag atacttgtcg
      481 atgacctcgg tcgacggggt gccggggtag ccgtcggcgg catggtagcc ggcgtggaca
      541 acgcccaagg cgaacgcctc gttcccctgc agtatgtgac gcgaacccgg agccgatgcg
      601 atcagttctc tgaagcgtga catggcgacc tcgcgagcgt tagagtgaac aatgaccgtt
      661 cgaacggtat cacgccccgt tcgggactgt caaggaaaaa gggtcagcgg ggggcattgt
      721 cccattccgc cagtcgcgtc ttgatgaatt cgaccaccgc gtggtgctcc tcggtgccgt
      781 tgcccgttat cgcgagcggt gcgccgaaat ccatgaacac gggtcgctcg cggtggagcg
      841 ggccgaccac cttgagcagt ctgaagcggc tattacccca gaagtcggtc ttgatggcga
      901 ccggcatcag ccggacaccc gccgttttcg caagttttac gccgagcgaa ttgaactgcg
      961 ccggatcgaa ggtgtcggaa cgggtcgcct gcggaaagag gatgaccgag gtcccctcgc
     1021 gcagatgcgc ggccccttcc tccagcacct ttttcaggtc gtcgcgcgga tttttccgcg
     1081 acacgacgat ggggcgggtc gccgccatga tgtcgcggta gagcgggttg cggaggatgt
     1141 tctccttgat gacgaaggtg acgggccgca ggccgccgat gatgccgggg aacaccatcg
     1201 tctcgagcgt cgacatgtgg ttgctcgcga tgaccagggg ctcggcggga tcgcgacgca
     1261 gatgatccaa gcccttcagg cggaaccggc acccgaccga ttcgagcagt cggaaggcct
     1321 cgatgctgct gcgggcccag acggcgcggt cgtaaccgcc ggtccgcgcg atcgccgagt
     1381 aacgtcgctg gatgtcgagc atcataagat agaagtacca gcggcttccg aggagcagcc
     1441 ggtcgccgat cggccgcgcg atgttgtcgg gcgtatcgta ggcgtcgccg gggagaaagt
     1501 tcgggatatc catgggtgcc tcacaccgtt ctgtcgtcac tatacgcggt gtgcccgcca
     1561 tttgcaagac ccttcttgac ttggctcggc accgacatac agtaggggac atgagcgagc
     1621 ggaccttcca tatcgacgaa gagcgtaccc tgcgtctccg cgaccgcgcc cttgccgcca
     1681 cggcggccga catcgccgcg gtgctcgggc aggagcatct ttcgcctgac gagcttgcgc
     1741 cgcttctctc cccggcgatg gacgacgcgc tttttatgcg tcttgccgcc gagagcgcgc
     1801 ggctgacccg ccgccggttc ggctacgcgc tgtcgctcta catcccgctc tattacgaca
     1861 accgctgcat caaccgctgc cgctactgcg ggttcaacgc cggcaacgag gtgccgcggg
     1921 tctttttgtc gcgcgaggag atactgcggg agggccgggc gatacgccgc cacggcttca
     1981 ccaacctcct gctcgtcgca ggggaacacc ccgaaagcgc cgacccctcg ttcctcgtcc
     2041 cggtcatccg cgatctgcac gacatcgggt tccgatcggt ctccatcgag atagcgccgc
     2101 ttgaggaggc ggcgtaccgg cggctcgtca ccgaagcgca tctcgacgga ctctatgtgt
     2161 atcaggagac ctatcaccgg gccacctatg ccgccgaaca tccgcgaggc ccgaaaagcg
     2221 atcccgaccg gcgactcgcg acgccggaac gaggggcccg ggccggcatc gcgaagatcg
     2281 gcatcggttt cctgctcgga ctgtatgatt ggcgctacga aacgcttgcg ctggcccagc
     2341 atctctcgtt cctgcagaaa catcactggc gcgccgagtt ctctataagt ttcccgcgca
     2401 ttcgcaaggc cgaaggggtc gcgggcggcc cgcacccggt cggcgaccgc gaattcctgc
     2461 agatggcctg cgcgctccgg ctcctgttcc ccgagacgcc gatgtcgctc tcgacccgcg
     2521 agtcgcccgc gttccgagac gccgccgtac cgatcggttt caccaacctg tcggccggca
     2581 gttcgacggt ccccggcggt tacgggacgc gaaagggcgc gctcgcccag ttcgaggtcg
     2641 aggatgcgcg cggtcccgcc gaggtcgcgg cggcgctccg ggcgcgcggt ttcgatccgg
     2701 tctggaaaga ctgggagtga cggtcgctgt gtcccggcgg ccgctcagcc ccggggtatc
     2761 gttatgatga aacgggcgcc gccgagcggc gaatcggcga ccgctatgtc cccgcccgcc
     2821 tcggtgacca gtttaagcga atgagacagc ccgatgccgg tgccgcccgg tttggtcgtg
     2881 tagaaattgc ggaatatctt ctcccgttcg gccgccggga cgccgcggcc gctgtcatcc
     2941 acgaacagtc gcaccttccc gtcggcgata tcgcccgata tcgccacggt gccgcgccgg
     3001 tcaagttcct cccaggcgtt cttgagtatg ttgagcagga cctcgcggaa cgagttgaat
     3061 gctatcccga gttcggcggc gggcagatcg ccgaccgtca gcgcgatgtc acgggtgcgg
     3121 aactcgctct catggaaccg cgtgacctcc gccaccgcgt cgcggagcgg agtgcgggaa
     3181 ggcgactcct catcggcgcg gtaccggacc atgcccatgt agcggtcggt tatctcctcg
     3241 agccggatga cctccttgac gatgagcgcg atggatgccg acagttcttc gggggaaagg
     3301 tcgtgtgcgt gatcggcgac atactcgaca ttgagtttta tcgtcgagag cgggttgcgt
     3361 atctcgtggg tcacctttga ggcgatctca ccgatcgata tgagccgttc ggcgctcatg
     3421 agacgattcg agagccggtc ctgctcggtc atgtcgcgaa caaggtggag caccgcgccg
     3481 cccacctcag tgatgtcgcg ccagtagacc gtgtcgtgcg ccgtgagttt gcccggctcc
     3541 gctatcgcca gcccgctttt ctccgccagt gcggcgaccg tttctcctat ctggtcctga
     3601 ccgacggcga accggtcgcg gaaagtgcgg ttgatgaact gtacgcgccc cgaagaggcg
     3661 tcgctcacca ccacgccgtc cgggagggaa tcgagtatat cgcggtaccg tttcaggagc
     3721 agttgttcgc gccggtgctc tttcggcgtt ggtgtcccgg cggggttgtc gggcggcggc
     3781 tcggccggga cggtgatcgt gtcacgccgt tggcgggacc gccacgccgc gatgatgccg
     3841 agcagtccga tggcgatcgc gacggcgaac gacgcggcgg cgaagcgggc cgagcggagt
     3901 atcgcgccgc gccgctcggt gagcgattcc ttctcgagtc gttttgcggc gagcaggacc
     3961 agtttgcgga gcgccctcag atcgcctcgc aactgtgccg gatcgacctc tccggtcccg
     4021 ggtgtggcgt tccagcgttc catcgccggc cagatccgtg cgtgataatc ggcgaagcga
     4081 ttcttgagca gaggatcgag gtcgcccgct atctcgccaa agatggtctc gtaatgttct
     4141 ttcagtcggt agagggccgg tcgaacggtc gcgggatcgg cgctttcggc gttcgtcagg
     4201 gtctcatgca ggatctcgag gtcgcgggcc atctcggcga cacggaattg ttgcgtggac
     4261 accgccggca tccggtacgc atcgaggaac aggaggttca gcaggagaaa gccgcccgcc
     4321 gcgaagaaaa gtacggcgag gagcgaaaga atgagtgcgc ggttactcaa agatcgtggc
     4381 cgtcgcgata tcgccccgct cgatgcccag aatgctcttg gtgatgatgg cgaccgaggt
     4441 gtgcgcctcg gtcagaacga ccaccacctc gccgatcggc tcgagcggca gtttggcgag
     4501 gtcatcgtcg tcgagggtat cgagtccgtc ctggcgacgg tgtatggtca acatgttccc
     4561 ttcgacgagt ccctccttgg cgcccttatc gagatagacg agatacccgt cgccgaacat
     4621 cgtcaccgga tcgtaccccg agaggacgat cgtctgcagt ttcttgttgt cgagttgctc
     4681 caccttgatg ttggggcggc gctcctggaa ttcgcggacg ctgtcctcgc gctggaccgc
     4741 cgtgtaggcc ttgatgatgc ggaccgtcgc gatgccttcg ttgttgatgt cgatgatctt
     4801 gcccttgccg gttatctcga tatggtttcc gaccacatcg ttggtgaccg gatgggtgat
     4861 ctcctcgacc acgcggaaga actcaagata gtcgccgacc gcatgcttct tccccgtgaa
     4921 ctgcacatag caggagtcct cggtggcgag cagttccttc gtgtcgaagg ccgccatgat
     4981 ctttcccgcc tgcttgagcg tttcgtcggc gacataaccg cggtagggga catcgagcac
     5041 cgtgtcctcg atctgggtga agcggtcgac gcggtatttg ccgcccaatt tcacatagtt
     5101 cttgtagttc tccggcttgt cgtcgagtgt cttgccggtg cgggccgttt cgtcggtctc
     5161 aagatcctcc gacgattcac cttcgccgcc gatcgaaagc ccgccctcgt tggtcggggg
     5221 catcataatc tcaccggtac cgcgaaagta gaccttgtcg ccgggataga tgaggtgcgg
     5281 attctttatc tgggggttga tggaccagac cttgggccag taccacggac ttttgaggta
     5341 cttttcggaa aggtcccaga gcgtgtcacc gctggtgacc tcatgctcgt cgggggcctg
     5401 cgtgaaatcg gccttctcca catcgatgaa cggagggacc ggcacctctt cgacgctccc
     5461 cggcgcctcg taggcgacca gcgacagcga gaacaaaaaa gcaagtgaga tcagcaggtt
     5521 cttcatgatg ccctcacact tacataaagt tatatgtaat tgttgtacaa tttcatgcta
     5581 attatataac aatcatttat agtaaatgtc aatttttcgg ctcatttttt atctcccggc
     5641 ttttcaggag cgtcagggtc tgtcgttttt tggtcccggc cccggtgtcc tccacgaagg
     5701 tctctatctt cacgatgccg atgcggggaa cgaaataatg aagagatttc aagaatatgc
     5761 ctttctgtgg gttgacggtc gtttcgacga ccaccgcccc ctcgaagttc ccggcgggcg
     5821 tttccaccgg atcgtcgatg gcgacgatgc tccgtgtctc gttgcgagtg gggccggtga
     5881 gggcgaccca cgcccgtttc tcctgcaacg ggaaggtgag caggtagcgg tcgcggtcgc
     5941 ggatcccgtg ccggtcggcg gtgagcgcgg tgccgcgatt atcgatgaac cagccattct
     6001 cctcgcgctc tatccgtatc gtcatctcgc ccgtctggcc gagataatcg accgtgtagg
     6061 tccaactgtt acccaccgcg agcggcaggt agcgcagggc cgattcgcga tagtcctgcg
     6121 gggtcttggc ggggtcggcg gtgccggtcg aggcgcaggc ggccgcgacg aggaggcaga
     6181 gcgaaaagac gacgactctt ttcatgacag ggggtactcc ttcaggttat cggtgccgta
     6241 tcgctccgcg aggagcgaca ggagatctcg cgcaagttct tcgtgccggg gagagcgccc
     6301 cagttcccgt ttgatcgatg tcacgccacc gtcggtgatg ccgcacggga cgataccggc
     6361 gaagtgcgcc ggatcggggt cgatgttgag cgcgaagccg tgggcggcga cccctttttt
     6421 cacatggacg ccgaccgccg ctatcttgtt gcggccgacc cagacgccgg tcttcgtttc
     6481 atcccagcgc gccgggatgc cgtaacggtc ggagagcgtc cgtgccagac cggtccccca
     6541 gaagacgacg aagtccctga tcgaggcgaa ccgggcacgg cgcaggtcga tgatgggata
     6601 tatcacgagt tggccggggc cgtggtaggt cacatcaccg ccgcgccggg tgcgatagag
     6661 ggcgataccg cgtcgctccc gttcctcggg gggcaggagg atgtggcgtt cgtcaccgcg
     6721 gaacccgagc gtgtagacgg ggcgatgctg gagaaaaagc acgatgccgt cgccgagttc
     6781 accgccgagc acctcgtcgc gcaggcgttc ctgcaggcgg agcgcctcgc cgtactcgat
     6841 cggctcgtcg aacaggtgcc agtagagcgt catcagcgtc cctccagcag tcggtcggcg
     6901 aagctgcggg gcagtttgag tttccgtatc tgagaaacgg tccgctcgac cgcgtactcg
     6961 acccggtgga acatgacggt catcttttcg gtgtcgaagg tgacaaagcc ggcgcgtcgg
     7021 tcgccatcgc gcggctgacc gaccgacccg atgacgcaga ccgatggata gggaaaatcg
     7081 atatctatct ggcccgaaga gagatcgagt tcgatggtgc gcccttcgcc ggtcagcacg
     7141 aaataactga acaggtgcga atgggccata aaggtgacgc gccgcaggaa cggctcgatc
     7201 tcgtacagcg gctcgaccgt gtccttgctg tagatgtaat cgaactgttc aggctggcgc
     7261 ggtgaaccgt gcgagcagag caggagtccc gagtcgtcgt tcaggacata tttcagtttg
     7321 ccgaggaagt ccttctgcgt atcgtcgagt tgctcgcgcg accattcgat gacatagcgg
     7381 atggcgtcga agtagttgga tacatcggtc ttccggacca ccccggcgtc atgattgccg
     7441 agcacggcgc cgtgtacatt gcgccgcagt atctcgaccg attcggcggg ccacgggccg
     7501 tatccgaccg cgtcgccgag gaaatagagc cggtcgggtc gcaactcgac gatgcgccgg
     7561 atgaccgcct cgagcgccgg caggttcccg tggatgtccg atatgagggc gatcagcatc
     7621 ctacagcaac tccgccgcag cgttcgcgag cgccgaccgt tcccccttct cgaggatgac
     7681 atggccggca agctggaatt ccttgaattt atccacgatg tacgagagcc cgttgttcga
     7741 ttcgttcatg taggggttgt ctatctggaa cggatcgccg gtcagcacga tcttggtgtt
     7801 ctcaccggcg cgcgaaacga tggtcttcac ctcatgcggc gtgagattct gcgcctcgtc
     7861 gacgatgatg aactgctccg ggatgctgcg gccgcggatg taggtgagag cctctacctc
     7921 gatgacgccc gaactcatca ggtcgttcat cttgccgagg ttggccgagt cataaccggc
     7981 aagcaggtat tcaagattgt cgaacaccgg ttgcatccac ggcttgagtt tctcgttgat
     8041 ggtgccgggc aggtacccga tatcgcggcc catcgggtag atggggcggg tgacgagcat
     8101 gcgggtgtac accccctcgt cgaggcactt cacgagtccc gcggcgatgg cgagcagggt
     8161 cttgccggtg ccggccatgc ccatgagcga caccagtttc acgcggtcgt cgagtagcag
     8221 gtcgagcgcc agtttctgcc cgacgttgcg cgccgatatg ccccagaccg acttgtactg
     8281 cttgagcggc acgaggcagt tgtcgaacgg atcgatgcgg acgatggatt ggtgctgttc
     8341 gttcgcgagg tccttgagga tgaagaactg gttcgagtac cccttgcgga acggttcggt
     8401 cgccgggtcg tcgagaggca gtttcttctg ctggtgtatc cgatcgatga tcgcaccgtc
     8461 cacatcgagc ggggcgaagc ccttgggaag gctcgtgaag ttgaccgtct cgttcttgta
     8521 ggtctctgcc tgtatcccga gcgcatcggc cttgatgcgc atgttgatgt ccttgctgac
     8581 gagcaccacc ggcacgccgg ggttctcctt ggtgacgcgg taggcgctcg agaggatgat
     8641 gttatcggcg tgcgagccgc cgaggtcgag gttgggcagg tcgacctgcg acgcgaggac
     8701 gatccgtatc gaaccaccct ctttgagttg caccccgtcg agcagactgc cccgcttgcg
     8761 gatgctgtcg atgtatcggg cgaacgagcg ggccgcgagg ccgatctgat tcatgtcctt
     8821 cttgaacttg tcgagttcct cgatgaccac gagcgggatg acgatgtcgt tctcctcgaa
     8881 gtggaatatc gccttcgggt ccgacaggat gacattggtg tctatcacga agatcttctt
     8941 ggacatgcgt tcctcctccc gggttcccgg acctcagttc tgcttgagat cgacattgac
     9001 cgtgatgtca cggtcgagcg ctatcgccat gggccgtatg ttgaaatcgt ccttctggaa
     9061 gccgagcacc atgacccccc cggcatcctg cggcaggcgg gtcttgcacg gcgtcgtgca
     9121 gagcaccttg ccggtctcgt tgttcaccac ggtgaccccc gcgaggttgg agttgacctt
     9181 gagcagtatc gttctcttgc ccgaccgccc ccccttctgg gtctgcgagg gctggtggat
     9241 ctttttctcg ggttccggtc gaggcttttc ggggaccggg tcgggttgtt tagggagggg
     9301 tttctgagtc tcttgcggcg tccgttcggc gaccagcaca cggtccggcc tgatgaatgc
     9361 gcggtagaag aggatgccgg cgacgagcag gagcgccaga atgatgacgg cggtgaccag
     9421 caccgatttc ttgcgcgtcg gggcaacggt atcgctccgg ccccggcccg ccatggtctc
     9481 cttgttgagc atcctgagca tgtcgtgctc tgaaggggga gggagcgccg tgtcgggcgc
     9541 ctcgccctcg gcccgcagga ttacggtcga gttcaggagg aacgcgaggt ttatctgttt
     9601 ttcaagtttg tgctgaccgt acaccttctt gaggtcggcg agcagatcga gcatcgtcgg
     9661 gtagcggttg ccggccgtct tctcgagaca gcgcatgatg atggcgtcca tctcgggcgg
     9721 gaccccgtgc tccttcgacg agggcaggtc gggtatctcg tactggtgct tgtgcaggac
     9781 cttcatgtag ttctggtcgg agaagggcag tttgccggta ctcatctcgt acatcatgac
     9841 gccgagcgag tagatgtcgg tccgcgtgtc gacctgctgg ccgcccgcct gttccggcga
     9901 catgtattcg ggggtgccga agatggcgcc cgccttggtg agcgtccgtt ccttgcgcga
     9961 ggtgacgccg gtcccgatga tcttcgagat gccgaaatcg acgatcttga cgaagggcga
    10021 tatctcgttc ttcgaggtga tgaggatgtt ctccggcttc atgtcgcggt ggacgatgcc
    10081 cttgctgtgg gcgctgtaga gcgcgagcgc tatctgggcc gatatcgcga tgacgtggtg
    10141 gagcgggagc ggtgcgcgtt ttttgatgat gtcggcgagc gactcgccct cgacatactc
    10201 catgacgaaa aaggcgttgc cctgctcggt gtagccgaaa tcggtcacat cgacgatgtt
    10261 ggactggccg atgttgctgg ccgcgatcgc ctcgcgcttg aaccgctcaa cggtatcctt
    10321 gttcttcgag agttcgaaat ggagtatctt gagcgcgacc ttcttcttca ggagcacatg
    10381 ctcggcgagg tagacattcc ccatgccgcc cgacccgatg aggctcgtga ccacatagcg
    10441 ttcgctgacg gtctggccga tgaacgggtc tttcttccct ttaagttcca gttcccggga
    10501 gaggctttcg ggtaccggcg tcggccgggg gatgtttggg tccgtcatgg gcgcacccta
    10561 ccatttcaac ttcggtttgt caaaaatata aggggtaaag gggcgaacgg ggctacttct
    10621 tctccgtccc ctcgggtggc ggcggggcga gaaggttctt gaccgtcacg aggaactgca
    10681 ggcgcagttt cgcctcttcc ggcggcaggt cgagcgagcc catgttatcg acgatgaacg
    10741 aggcgcggtc gagcgcctgc ttcttctcct cgtcattcac cgtcgcgccg tgctcgatgc
    10801 ggttgcgcag gtcgatgaga gagtcgagat agcgttccga caggctccgc gtgagcagtt
    10861 tgtggttgtc ctcgagcgcc gccttgtagt cgcccatgag cttcatctgc tcctgtcgcg
    10921 cggtctggag ttcggcgcgg gtccgggaaa gggagaagta gagatagatc gacagcgcga
    10981 cggcgcaggc gagaagcacg gcgagcaggc gggccgcccg gtcggggcgg tcgccgcggg
    11041 gttccgatgg ggtcatgagg cctccgggaa aaagttagaa cacgagcgaa cagccgcagc
    11101 cgtcgtcctt cttggccggg gtatcggtgt cggggaccac cgtgccgtcg gcgaggtcgt
    11161 cggtcgccgc gatatcgtcg ccggccaggt cgtcgtcggt cgccgcgatg tcgtcggtca
    11221 cctcgctctt attgacgcag gtgccgttgt cgcaggtcgc gttctcgtca aaacactcct
    11281 cgtcggacga gcaggacatg aagcagacat cctccttacc gttgagcagg ccgtggcatt
    11341 tgagttgttc gccgcccatg ctgcggcagt cggcatcctc ttcgcacggc gggacgcaca
    11401 tatccgcctc gcccatgctc gggatgttga catggacgca cttggcgccc tgatcgccgc
    11461 agtagtcgtc tatgccgtct tcgcacgaga cataacagaa gccgcccggg accatcgtga
    11521 cgcagtagcc gttgtagagg cagtcctcgt tcgtttcgca ctcgccgccc ggctcgctcg
    11581 ggtcgtggcg gcagtggccg gagggatcgc agtaggattc ctcggtgcag tcgtcgttcg
    11641 tctcacacag ggcggtgcag aacccgagat tcgcctcggt gcagtagaaa ccctcgcgtt
    11701 cgcacggatg gtcgtcgttg cagtccttga ggcagtaggc gccgccgtcg cccggggcgc
    11761 gggtgccgtc ggggcagtcg gtcttgaccg gcaggacggc gcagtagccg ccgtcgtact
    11821 tggtcgcgcc gtgggcggca aggcagtcga catcggcctc ggtcaggtcg ccggtcacgc
    11881 agtcggcctt ctcggtacat gggccgccga tggtcgacag gtcgagcgcg aacccttcgt
    11941 cgacccagcc gtcgcagtcg tcgtcgaggc cgttggcggt ctcggccttg atatcggccg
    12001 cgtcgcacag gtcgagcccc gccgcgagcc gcaccgcctt gaggaggttg gcacggccga
    12061 agccgaactt cgggctgcgg gcgtcgccgg ccggcgcgtc gagcggttta tcccagaagc
    12121 cggtctcggg gtttatcttg tcggaggaaa gtttgacgat atcgaccgcc tcctcgagcg
    12181 tgagcgtcgg atcgaccgag aagatcacgc ccatcacgcc ggtcgcgaag ggggccgccg
    12241 ccgaggtgcc ggagaaacgg ttcgagtaat cgtcgtcgag atcgccgtat gtctggatgt
    12301 aggtcgtcgc gatgccgagc gtcggcgcgg aggagagggt cggggtgacc atcgcgatct
    12361 ccttgccgta gttcgaatag gacacgcggt tgtcccacgc gctcgtgccg ccgatcgaca
    12421 ggatggtggc gaccacatcg gtgtcgccgc gcttgaactt gaactcgtag ccgtgcagga
    12481 ccatgtagtt ggtgtcgacc ccgtcgttgc ccgcggcgta aagtatcgcg ccgccgagcc
    12541 cgccgcgccc gttctggagg aagttgaggt gcgattcctc ctcgccgggc atcagcggta
    12601 cctcgcccat gcccgccatc gggccgaacg agcagttgac cgcgacgatc ttctcgtcgg
    12661 ccacatattt ctcatacgat tcgatgtagc cctgttcgga actggccgag ccggatccgc
    12721 cctccatgaa gcagaccggg tagatgccgc accacgggca gacgccggtc gtcccgatgg
    12781 tgttcccctc ggccgccgcg acgccggcgc agttggtgcc gtgcgaatag ccggcggtgc
    12841 cgtacatgct gtcctctatc gtgccgtagt cgccgcccgc ctcgtggttg acggcgtccc
    12901 agccctcttc ggtcttgttc ttgaggtcgg ggtgggtcag atcgacgccc gaatccatga
    12961 tggcgacctt cgtcgcgccg tcgaacccgt cgagttcgtt cgccgcgatc cggtcgacga
    13021 ggaactgcat cgcctgctcg aagcggatgt cggcgttcgc cttggtcggc agttcggggt
    13081 tcgacaggcc gatggcggtg ccggtgttct tgagcgccca ctggtagccc tcggtgtagt
    13141 atttatcggt tatcggcatg accggctcgg cgaacagcac ctgctcgcgg accatgtcgg
    13201 ggaacgcgaa accgtcgccc gcctcgacga tgcgctgggc gagttccgcc gcgtcgcccg
    13261 tcacggcgaa ccggtacagg cgcgtcgagg ggtatatctc cttcagcgtg agtccgtact
    13321 tcttcgcgag ggcgttcacc ggcatcgcgc cgcgccagaa tatctggtcg ttggcgacga
    13381 acagccggtc gccgaaccgg tacacaggca gggtccccgc gttcgagtcg tccaccacga
    13441 gatagcggcg gtcgagtttc catgtcgccg cgggcgcctt ggcgccggtg aggtccgcct
    13501 tggccgcgaa ccgcgccgtc agcgccgggt ccttggcgaa ctcggtgaaa gagccgtcgc
    13561 tccggtagac cttcgccgcg aagagcgagg acccgaaaag aacgacgagg aaagagacga
    13621 tgatgcgacg catgagagcc tccatatcaa acagccaaaa atgttctgtc cacctctgct
    13681 gatgacgcta gtggagagcg ttgccgatgt caaatacttt tttttatctt cccattgcct
    13741 tttcggggtc gttccctaca atgaggaaaa aaggtagacc acttttcagc gaggtgcatc
    13801 atgcgacatg cgttctttgc gctccttatc ggtgtggcgg tccttccgtt cctctcctgc
    13861 acgccggtgg aggaagatcc cgggaccgac acccttgcgg tgaccgacag cgacgccgcg
    13921 gtcgaccccg acggaacggt cgaccccgac gggacggtca accccgacgg gacggtcaac
    13981 cccgacgacc cgcaggggga cggcgtcgtc accgacgagg ac
//
LOCUS       DAOQUE010000010        13844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_4987_length_13844_cov_4.544202, whole genome shotgun sequence.
ACCESSION   DAOQUE010000010 DAOQUE010000000
VERSION     DAOQUE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13844
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4987_length_13844_cov_4.544202"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKH10_00660"
     CDS             <1..663
                     /locus_tag="PKH10_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNT26662.1"
                     /translation="VLARTYAVHKKVMRPRTALYDLDPTVLDQVYGGLEKEDLTANKA
                     VADTRGEILSYNDLPAEIYFHSTCGGRTASAKEVWGKEVPYLVPVKCDHCKNSSLYRW
                     TRVLHADEVAKKLAAAGYAVGKLKKVSIEKGDLRVAAVIVNGKRIEVNQFRKVVGYTT
                     VYSNDFTITLSGGKLTFSGKGFGHGVGACQYGMAGMAQAGKTWREILQHYLPGAEIRK
                     MY"
     gene            663..1700
                     /gene="queA"
                     /locus_tag="PKH10_00665"
     CDS             663..1700
                     /gene="queA"
                     /locus_tag="PKH10_00665"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNT26663.1"
                     /translation="MDVSRFDYELPEELIAAEPLPRGASRMMLVRRDNSTVEHRRFAD
                     ILSLVDENTTIVLNESRVLPARFFGKKGTGGAVEFLFLGARPDGTCDTLAKSSKQLRP
                     GEVVTLNSGDTVEIVDRIGESCRIRTSFPATAMPAYLEKNGDMPLPPYILKRRGEKRS
                     RPEDRESYQTVYAGGAGSVAAPTAGLHFTDTMLDLLRERTGGHVERVTLHVGIGTFMP
                     VKVDRVEEHVMHREFYYIAPETTARLNSDKATGRRILAVGTTTVRTLETAAAGGMLAV
                     GGGSSQLFIYPGYSFRFVDAMLTNFHLPRSTLIMMASAFAGYELTMQAYREAVAERYR
                     FFSYGDCMLIL"
     gene            complement(1695..2015)
                     /gene="trxA"
                     /locus_tag="PKH10_00670"
     CDS             complement(1695..2015)
                     /gene="trxA"
                     /locus_tag="PKH10_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNT26664.1"
                     /translation="MLALNKDNFTTEVEQFDGLVIIDFWAPWCGPCRMLGPVFEELAA
                     EYAADPKVKFAKVNTDECQDLAVRFKIRGIPTIKIVKNGQEVDQFVGAGPRSTYQELI
                     KKHL"
     gene            complement(2037..2564)
                     /locus_tag="PKH10_00675"
     CDS             complement(2037..2564)
                     /locus_tag="PKH10_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26665.1"
                     /translation="MRTIIGSLVMLFVVGTFAQEATITTASGKTIKAKIAKIEAEKYN
                     GTGRAPANEFAVVQGNSEYLLSFDKISSLKLLETDDMSCYEDSAYKPTREFCSKKLVY
                     EVKLKIPDKNKQKIEIVDERKFIFTLAGQPDPIVTFFYKIQASDEGKEAETNLKQIEN
                     MVKDFQQNGIKSIKF"
     gene            2751..3632
                     /locus_tag="PKH10_00680"
     CDS             2751..3632
                     /locus_tag="PKH10_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26666.1"
                     /translation="MFLVPVLALSFVLSCASAPQEEEAAPKKKKQVIDDGNNPFTEES
                     VNFKNGDKYIGEVKNGLMEGKGKYLFKEGKVYEGQFFNNRFNGEGTLTFPNGDIYTGQ
                     FKDDQMHGTGTMRFKSGNVYEGAFVNDIIRGKGVFRYIDETQYNGDFENGKRQGKGTL
                     SYPNGDLYDGEWREDKKHGYGVMKYTSGERYEGTFEDGIVHGQGTYTFKDGKVYEGEF
                     KNGKIAGEGRLTMKNGCEYTGGFKNGSYEGHGVLTCPGAQRLEGTWKKNKLVQDIEKD
                     EDESSEGEKDGEEEQEE"
     gene            complement(3652..5568)
                     /gene="pepF"
                     /locus_tag="PKH10_00685"
     CDS             complement(3652..5568)
                     /gene="pepF"
                     /locus_tag="PKH10_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNT26667.1"
                     /translation="MKIVIACAVAAFLSVAACHSSAQQPGAQSQQVDRANIPDKYKWT
                     PSHIYATPQDFEADFDTASKIVLQLKGFSGTLGNKESARTALKTYFEGMRTLYNLETY
                     ASRIKDSDMNDVEAQKIAGRVETLFTSFIAAASFLQPEILKLSDETIKDYLADETFKD
                     LRRPLSEMARWKPHTLTVAEEKVLAEFTGTAYGPYEAYTTFTTTDMPRPDITFLNGET
                     VRLDDAMYVKYRTSFDRNDRKLLFESFWDTYGHYQNTFSKTLTYQTKYYTTAARLKKF
                     SSSLESKQFEKELPPDFYDKLIAHIRSILPALHEYMAFRKEMLAVDKVRPYDMYVPLT
                     TAEYKKHYSYEEAADIINAAVSPMTDEFKEAIKKGMTPGSGWVDVYPSKGKADGAYCA
                     SGTVTNHPFVLLNWTDDYDSLSTMAHEMGHAMHSYFSNKYQPFPTADYNLFNAEAASI
                     FNEILLSAHLLKNTKDTEERIFLLNNFVEMTRGTVFRQALFAEFEKRFYDVVEQGSPL
                     TPDVMKKLYKEINEEYYGASKGLYEMEDRYAVEWSYIPHFYYNYYVFQYVVGFVAALT
                     LGTKVIEGQIPAAQYVDGLLSAGGSKSPLEIFRDAGIDMTSDEPFKLTEKIFRERLSE
                     LRELLKAQKTTSQK"
     gene            complement(5613..6011)
                     /locus_tag="PKH10_00690"
     CDS             complement(5613..6011)
                     /locus_tag="PKH10_00690"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HNT26668.1"
                     /translation="MDEQTKRDKDDVIYTRSSKRVDVELGVSFEMADEGPHKFYTGIT
                     QDISQGGVFLATHRILPIGDKVRLTFSLSGREISAQAEVVWSCDSVTAATYTGPGMGL
                     RFMSISEEDKRFIEEYVKQRETIYYDDGEL"
     gene            complement(6105..7850)
                     /locus_tag="PKH10_00695"
     CDS             complement(6105..7850)
                     /locus_tag="PKH10_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT26669.1"
                     /translation="MKIVNIVDLNLVKIFLSKFYLAHGLDYLFIVPHKETIEKQIPLF
                     QPDLILLQHQYSEAPVEDIVAQVRALTPAPIAVFFLRSDKETVKRFSSLGKKGKDLFF
                     YDKHSFAQEFEDLLNRQTTGISREQVAAENEVRHSLLFADDSAVMRHFVRRALADRPI
                     TLHVADDGAEAWDLYQEHLPNLVITDIEMPRMDGLQLCKKIKENNQGRFIPVIILSNK
                     KKPIDIDTAFDIGADDYLTKPTTTEEFNHKIDEYLSVLDRKQRNKILLADDSKVSSEI
                     ITHALVKNSLQVLHAVDGETAFDLAVRERPDIIITDIEMPRLNGYELAKKVRQHPDLK
                     ETSLIMMSARDRRSDIKKGEKLGISRYFIKPFDVEKLVIVVEQLLLEKYNIYQKEYEY
                     MLSTIQSLITALEARDHYTKGHTQRVSAYASRLGRFIGMSSYALKELEIGSNLHDVGK
                     IGVRDDILLKPGRLSAEEYAKIQEHAIIGAEILRPLKLLENIIPLILFHHERWDGRGY
                     PSMIHGDKIPLGARIIAIADTYDAITSDRPYRKALSQEDALQIIRENLGKQFCPETGR
                     AFLAMMETEGATLPK"
     gene            8098..9018
                     /locus_tag="PKH10_00700"
     CDS             8098..9018
                     /locus_tag="PKH10_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNT26670.1"
                     /translation="MLGIKEIGIYIPVGREPNHDKKEKWGIDDTFLRDKIGVDRHAFR
                     DPGEEATDMGYKAFQHLASKIDIDRTKVEVLVFVTQNPDERMPHAGTILHEKIGLPYT
                     CASFDIPLGCSGFMYGLSVITGFMKENGMTRGLLITADPYTKCVNPEDKNTSLLFGDG
                     ATATYITDDPVLVPGKYVFGNLSEKWREIKLVDNWLYMNGPAVVKFVAKYVPENVRRT
                     LEKNNLKKEEIDRWIMHQASRFMHKVISENLDISPDKMPWDCRDYGNTVSSSIPLLLE
                     KELHNKQHKHYFICGFGIGLSWGSSVLRRV"
     gene            complement(9015..10205)
                     /locus_tag="PKH10_00705"
     CDS             complement(9015..10205)
                     /locus_tag="PKH10_00705"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNT26671.1"
                     /translation="MSIGARTHLFFFALLLPLLVEARGQVGLDVLEARGFDAIAGERV
                     ALVVNHTARNADGKHIIDLLLDGKKATVVRIFTPEHGLTGLSDEAVADSTYRNVPVVS
                     LYGKEKKPKPELLTDVDAVVFDVQDVGARYYTYPATLAYTLEAAKAAGKKVIVLDRPN
                     PAGGSVVAGFVPPVELTGKFTSLYSIPTRHGMTIGELARLFNDHFGIGATLEVVAMSG
                     WTRTMLWQDTGLPWVPPSPNLKSPDAALIYAGMGWIETTNISVGRGTEAPFFLYGAPF
                     IDGTKWAAALKKALPAGVSAAPATFTPADKLHRHFGKECGGVRLTVNDLSADLFAAGL
                     AMLRTLSTLFPDFDPTSDFAISMGKAGIDKRLKNGETVKKILAEAAADCKKFKKIRKK
                     YLLY"
     gene            10331..11215
                     /locus_tag="PKH10_00710"
     CDS             10331..11215
                     /locus_tag="PKH10_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNT26672.1"
                     /translation="MPYFFVTLLIFSSMEVVSKPLMPFVDPFQLTFHRFLVGLIFLFL
                     FLVARGRCHDLIRIGRHDMLLLAFLGFLNTFFAMSMLQAAVKNSTAATAATVICANPL
                     FVYLFSLVTGDESFSWRRIAGIVAGMIGIALVMFDQGLRFDVGVLYALAAAAAFALYT
                     LLNKPVARRIGSLPVNIVSFSFGILLSAVYLLVTGVGVLPPLEVTNEPARLAAFLYLG
                     VVVSGIGYVTFVKTIRALTPVAASVIFIVKPALATLFAVMFLGERLSALFWPGLLFVV
                     LGGLLILRNAPSQKVDTH"
     gene            11261..13264
                     /locus_tag="PKH10_00715"
     CDS             11261..13264
                     /locus_tag="PKH10_00715"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNT26673.1"
                     /translation="MTQASDPAQVYRHLVRILGALVGSGTISQLVSSLEGGLRALTPH
                     LFLGVAIWNRYEDELVTFLPRGRRLERGLRRYFEAIEASLHDAGTGRYGEGALFERPV
                     QRAGHSFFYERRAVEEGVTAMLFIEFVSPEDGDSFGLIAGDLVPQFWGLLAERYGKVA
                     AGEESDMLWRLTDAEKREEDWLNEIELNQLLQNLLQLSLRKLGAKIGAILLLDERTGD
                     FIIEPRAVVGKAISVIPEKFSTKERSITAIVYRTNQYYICNDTESDGNYYPIFQGIKS
                     SLIVPITFQGRCIGVIAIESQRKGRFSVSDAENLQSIAKTATMFIRRAQLYRETAQSG
                     DGIMIFGRTEKWKEVERRIEKAARTDATVILRGESGTGKELIAHAIHFNSPRRDRPFV
                     VINCAAIPSELLESEMFGHVKGAFTGAYADKTGEFERADGGTIFLDEIGDLPVLLQVK
                     ILRTLQSGEIRPVGSSKPPQRVNVRVIAATSRDLERMIEAGTFRLDIYYRLHVVPIWL
                     PSLREYREDIPFIVEHFIREANERFHTTVQRPDKRALDLLMVYDYPGNMRQLRNFVQQ
                     AVIMSDGPVLRSEHLPPEVQGAAPLISASAGGSTAPRFSAEGPLPSYREEKERVLADF
                     SSEYLRALLARTRGNIKQAAREAGISRMALYKLLARYAIPHHD"
     gene            13331..>13844
                     /locus_tag="PKH10_00720"
     CDS             13331..>13844
                     /locus_tag="PKH10_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26674.1"
                     /translation="MKRGLAVVMVLVSCLFLFSCSSSEEDDPTGDAVVIADGDTVPDG
                     STVDGGDDLAGTDEDTAAQCTPNETAECYHGPSGSRDRGICKAGLATCGASGYWGPCE
                     GEILPQPEICHNDIDENCDGEDGTEENTADYDSDGYTYCTGDCCEFTSECQFPALVNP
                     AGYDFPGNGID"
BASE COUNT         2781 a         3838 c         4184 g         3041 t
ORIGIN      
        1 gtcctcgccc ggacctatgc ggtgcacaag aaggtgatgc gaccgcgcac cgcgctctac
       61 gacctcgacc cgacggtgct cgatcaggtc tatggtggac ttgagaagga ggatctcacc
      121 gcgaacaagg cggtcgccga cacgcgcggc gagatactca gttacaacga cctgccagcc
      181 gagatatatt tccattcgac ctgcggcggc cgcaccgcgt cggcgaagga ggtctggggc
      241 aaagaggtgc cctatctcgt tccggtgaag tgcgatcact gcaagaattc gtcgctctac
      301 cgttggacgc gcgttctgca tgccgatgag gtggcgaaaa aactggcggc ggcgggttac
      361 gccgtcggca agttgaagaa ggtttctata gagaagggcg atctgcgggt cgccgccgtc
      421 atcgtcaacg gcaagcggat cgaagtgaac cagttccgca aggtggtcgg ctacacgacc
      481 gtctactcca acgacttcac cataacgctt tcgggcggca aactgacctt ctccggcaaa
      541 ggcttcggac acggcgtcgg cgcctgtcag tacggcatgg cggggatggc gcaggcgggg
      601 aagacctggc gcgagatact gcaacactat ctccccggcg ccgagatacg gaagatgtac
      661 tgatggatgt atcccgcttt gactacgaac ttcccgagga attgatcgcg gccgaaccgc
      721 tgccgcgcgg cgcgtcccgg atgatgctgg tgcggcgcga taacagtacg gtcgagcatc
      781 gccggttcgc cgatattctc tctttggtcg atgagaacac gacgatcgtt ctcaacgaga
      841 gccgcgttct gccggcccgt ttcttcggca aaaaagggac cggcggcgcg gtagagttcc
      901 tgttcctcgg cgcgcggccc gacggtacct gcgatacgct ggcgaagtcc tcgaaacagc
      961 ttcgtccggg cgaggtcgtg actctgaatt cgggcgatac ggtggaaata gttgatcgga
     1021 tcggggagtc gtgccggata cgcacctcgt tcccggcgac ggctatgccc gcatatcttg
     1081 aaaagaacgg cgatatgcca ttgccgcctt acattctcaa acggcgcggc gaaaagagga
     1141 gccgtcccga ggatcgcgaa tcctatcaaa cggtctatgc ggggggtgcc ggttcggtcg
     1201 cggcgccgac ggcgggtctg cattttaccg atacgatgct tgacctactc cgggaacgga
     1261 ccggcggtca tgtcgaacgg gtgacgctcc atgtcggtat cggcaccttc atgccggtca
     1321 aggtcgaccg ggtcgaggaa catgtcatgc accgcgaatt ctactatatc gctcccgaaa
     1381 cgacggcgcg gctcaactcc gacaaggcga cgggccggcg gattctggcg gtcggcacca
     1441 cgacggtacg gacgctcgag acggcggcgg cgggcggtat gctcgcggtg ggcggcgggt
     1501 cgtcacagtt attcatctat cccggctatt cgttccgctt tgtcgatgcg atgctcacaa
     1561 attttcatct accgcgttcg accctcatca tgatggcgtc ggccttcgcg ggctatgagc
     1621 tgacgatgca ggcatatcgc gaagcggtcg ccgaacgcta ccggttcttc agttatggcg
     1681 actgtatgct gatattataa gtgtttcttg atgagttcct gataggtcga tctcgggccg
     1741 gcgccgacga actggtcgac ctcctgcccg ttcttcacta tctttatcgt cggaatgccg
     1801 cgtatcttga accggacggc gagatcctga cattcatcgg tgttcacttt ggcgaatttc
     1861 accttagggt cggccgcata ttcggcggca agttcctcga ataccggacc gagcatccgg
     1921 caggggccgc accacggcgc ccagaaatcg atgatgacca gaccgtcgaa ctgctcgacc
     1981 tcggtcgtga aattgtcctt gttcagtgca agcataggtc cctccgtggc tgatcgctag
     2041 aattttatgg atttaatgcc gttctgctgg aagtccttga ccatgttctc gatctgcttg
     2101 aggttggtct cggcctcctt tccttcgtcg cttgcctgta tcttgtagaa gaaggtcacg
     2161 atggggtcgg gctgaccggc gagggtgaag atgaatttgc gctcatccac gatctctatt
     2221 ttctgcttgt tcttgtcagg gatcttaagt ttgacctcgt agaccagttt tttcgaacaa
     2281 aactcgcggg tcggcttgta ggccgaatcc tcgtagcaag acatatcgtc ggtttcgagc
     2341 agtttgagcg aagatatctt gtcgaaggag agcaggtatt cgctgttccc ttgaacgacc
     2401 gcaaattcgt tcgcgggcgc tcgaccggtg ccgttgtatt tctcggcctc tatcttggct
     2461 atcttcgcct tgatggtctt cccgctcgcc gtggtgatcg tcgcttcctg cgcgaaggtg
     2521 ccaacaacaa aaagcatgac cagcgaccca atgatcgttc tcatgaaaac cccctgtatc
     2581 caagttactc gaccggtgtt cattataggg ccttggatcg aaatagcaag ccccttaagg
     2641 agaaagccgt cgctggggat ggctctgttt gactttccct gcttcggcgc tacactccta
     2701 cccggtgtaa ggtcatcatc aagagaggtt aacgcatgcg gagcgtatcg atgttccttg
     2761 tccccgtgct tgcgctgtct ttcgtactat cctgtgcgtc ggcgccccaa gaagaagaag
     2821 cggccccaaa aaagaaaaaa caggtcatcg atgatgggaa caatcctttc acggaggagt
     2881 cagtcaactt caagaacggc gacaagtaca tcggcgaggt caagaacggt ttgatggaag
     2941 gaaaaggtaa atacctcttc aaggaaggca aggtgtatga aggacagttc ttcaacaacc
     3001 gcttcaacgg tgaagggaca ctgaccttcc cgaacggcga tatctacacc ggtcagttca
     3061 aggacgacca gatgcatggt accggcacca tgcgtttcaa aagcgggaat gtttacgagg
     3121 gggctttcgt taacgacatc attcgcggga agggcgtgtt ccgctatatc gacgaaacgc
     3181 agtacaacgg cgatttcgag aacggcaaac ggcagggcaa ggggacgctt tcctatccca
     3241 acggcgatct ctacgacggc gagtggagag aggacaaaaa acacgggtat ggggtcatga
     3301 agtacacatc aggcgagcga tacgaaggca ctttcgagga tggtatcgtg catgggcagg
     3361 ggacctatac cttcaaggac ggtaaggtgt acgaaggcga gttcaaaaat gggaagatcg
     3421 ccggtgaggg acggttgacc atgaagaacg ggtgcgaata taccggcggg ttcaagaacg
     3481 gctcctacga aggccacggt gtattgacct gtcccggtgc ccagcggctg gagggtacct
     3541 ggaagaagaa taaactggtc caagacatcg aaaaggacga agatgagtct tctgaagggg
     3601 aaaaggatgg agaggaggag caggaggaat aggtgttccg gtccgacccc gctacttctg
     3661 tgaggttgtt ttctgcgcct tgagaagttc ccgcagttcg gagagccgtt ccctgaatat
     3721 cttttcggtc agtttgaacg gttcatcgct ggtcatgtcg atgccggcat cgcggaatat
     3781 ctcgagcggt gacttcgagc cgccggccga aagaagtccg tcgacatact gggcggccgg
     3841 tatctgtccc tcgatcacct tggtgccgag cgtcagcgcg gcgacaaagc cgaccacata
     3901 ctggaacacg taataattgt agtagaaatg ggggatgtac gaccactcga cggcgtagcg
     3961 gtcctccatc tcgtacagcc ctttcgaggc gccgtagtat tcctcgttta tctctttgta
     4021 gagtttcttc atgacatcgg gggtaagcgg tgagccctgt tcgacgacat cgtagaatct
     4081 tttctcgaac tcggcgaaca gcgcctgacg gaaaacggtg ccgcgcgtca tctcgacgaa
     4141 gttgttcagg aggaatatcc gctcctcggt atccttcgtg ttcttcagga ggtgggcgga
     4201 aagcagtatc tcgttgaaga tggatgccgc ttcggcgttg aagagattgt agtcggcggt
     4261 cgggaacggc tgatatttgt tcgagaaata ggagtgcatg gcgtggccca tctcatgagc
     4321 catggtcgaa agtgagtcat aatcgtcggt ccagttgaga aggacgaagg ggtgattggt
     4381 aacggtcccg ctggcgcagt aggcgccgtc ggctttgcct ttggacgggt acacatccac
     4441 ccatccgctt ccgggggtca tgcccttttt tatcgcctct ttgaattcgt cggtcatcgg
     4501 tgagaccgcc gcgtttatga tatcggccgc ctcctcgtag gagtagtgtt tcttgtattc
     4561 ggcggtcgtg agcggcacat acatgtcgta ggggcgcacc ttgtcgacgg ccaacatctc
     4621 tttccggaag gccatatact catgaagtgc cggcaggatg gagcggatgt gtgcgatgag
     4681 tttgtcgtag aagtcgggcg gcagttcctt ttcgaattgt ttgctctcca gagaggagga
     4741 aaacttcttg agtcgtgccg cagtggtgta gtatttggtc tgataggtga gcgtcttgct
     4801 gaaggtgttc tggtagtggc cgtaggtgtc ccagaaggac tcgaagagca acttacggtc
     4861 gttgcggtcg aaggaggtgc ggtacttcac atacatcgca tcgtcgagcc ggaccgtttc
     4921 gccgttcaag aaggtgatgt cggggcgcgg catgtcggtg gtcgtgaagg tggtgtacgc
     4981 ctcatagggg ccgtaggcgg tcccggtgaa ttcggcaagc accttttcct cggcgacggt
     5041 cagggtgtgt ggtttccagc gggccatctc ggagagcggt ctgcggagat ccttaaaggt
     5101 ctcgtcggca agatagtcct tgatcgtctc gtcggagagt ttcagtatct caggttgcag
     5161 gaaacttgcg gcggcgatga acgaggtgaa aagcgtctcg acgcgaccgg cgatcttttg
     5221 tgcctcgaca tcgttcatgt cgctgtcctt gatgcggctc gcgtaggtct cgaggttgta
     5281 gagcgtgcgc atcccctcga aataggtctt gagggcggta cgggcgctct ccttgttgcc
     5341 gagcgtaccg gagaacccct tcagttgcag gacgatcttg ctcgccgtat cgaagtcggc
     5401 ctcgaaatcc tgcggggtgg cgtagatgtg gctgggcgtc catttgtact tgtcgggtat
     5461 gttcgcccgg tcgacctgtt gagactgagc tcccggttgc tgagccgaag aatgacaggc
     5521 ggcgacgctt aaaaaagcgg cgaccgcaca cgcgatcacg atcttcatgg tatcctccac
     5581 attatgttgg tctagtgccc ggaacggtta tgtcagagtt cgccatcgtc gtaatagatg
     5641 gtctcccgct gcttcacata ttcctcgatg aagcgtttgt cctcttcact gatggacatg
     5701 aaacgcagtc ccatgccggg accggtgtag gtcgctgcgg tcaccgagtc gcaggaccag
     5761 accacctcgg cctgggccga tatctcgcgc cccgaaagag agaaggtcag ccggaccttg
     5821 tcgccgatgg gaaggatgcg gtgggtggcg aggaaaacgc ccccctggga gatgtcctgc
     5881 gtgataccgg tgtagaattt atgaggaccc tcatcggcca tctcgaagga gaccccgagt
     5941 tcgacatcca cccgcttgga actgcgggta tatatgacat cgtccttgtc ccgtttcgtc
     6001 tgttcgtcca tgcggcgctc ctgaaacttt cgcccaaaac ggaccgatta taagcgacgg
     6061 gcatgcggaa atcaacaaaa ccattctgtg ccgcgaaaca tcggttattt gggcagagta
     6121 gccccttcgg tttccatcat ggccagaaag gccctgccgg tctcaggaca gaactgttta
     6181 ccgaggttct cgcgaatgat ctgaagcgca tcctcctgag agagcgcctt gcggtagggg
     6241 cgatcggagg ttatggcgtc gtaggtgtca gcgatcgcga tgatacgggc acctagcggt
     6301 atcttgtcgc cgtgtatcat ggagggatac ccgcgtccgt cccaccgttc gtgatgaaag
     6361 aggatgagcg gaatgatgtt ctcgagcaat ttcagcggac ggagtatctc ggcgccgatg
     6421 atcgcgtgtt cctgtatctt cgcgtactcc tcggccgata ggcggcccgg tttgagcaga
     6481 atgtcatcgc ggacgccgat cttgccgaca tcgtggaggt tcgagccgat ctcgagttcc
     6541 ttgagcgcgt aagatgacat gccgatgaag cgaccgagcc gcgaggcgta ggcgctgacg
     6601 cgctgggtgt gtcccttggt gtagtggtcg cgtgcctcga gcgccgtgat gagcgactgt
     6661 atcgtggaga gcatgtactc atactctttc tggtagatgt tgtacttttc cagaagcagt
     6721 tgttcgacca cgatgaccag tttctcgaca tcgaacggct tgatgaaata gcgcgagatg
     6781 ccgagtttct ctcccttttt gatgtcgctt cgccggtcgc gggcgctcat catgatgagc
     6841 gatgtctcct tgagatcagg gtgttggcgc acctttttcg ccagttcgta accgttgagg
     6901 cgcggcatct cgatgtcggt gatgatgatg tcgggccgtt cgcgcaccgc gaggtcaaag
     6961 gcggtctcgc cgtcgacggc gtgcaggacc tgcagactgt tcttcacgag cgcgtgggtg
     7021 atgatctcgc tcgacacctt gctgtcgtcc gcgagaagta tcttgttgcg ttgtttacgg
     7081 tcgaggaccg agagatattc gtctatcttg tgattgaatt cctcggtggt ggtcggtttg
     7141 gtgagataat cgtccgcgcc gatatcgaag gcggtgtcga tgtctatcgg ttttttcttg
     7201 ttcgacagga tgatgaccgg gatgaaacgc ccctgattgt tctcttttat cttcttgcag
     7261 agttgtagcc cgtccatccg cggcatttcg atatcggtga tgacgaggtt gggcagatgt
     7321 tcctgataca ggtcccacgc ctcggcgccg tcgtcggcga catggagcgt aatggggcgg
     7381 tcggccagtg cgcggcgcac aaagtggcgc atcacggcgc tgtcatcggc aaagagcagg
     7441 gaatgccgca cctcgttctc ggcggcgacc tgttcgcgtg agatgccggt cgtttgccgg
     7501 ttgagaagat cctcgaactc ctgcgcgaac gagtgcttgt cgtagaaaaa gagatccttc
     7561 ccctttttgc ccagagaaga aaaccgtttc accgtttcct tatccgacct gaggaaaaaa
     7621 acggctatcg gcgccggtgt cagggcgcgg acctgcgcga caatgtcttc gacgggggcc
     7681 tccgagtact ggtgttgcaa caggatcaga tcgggctgga agagcggtat ctgtttctct
     7741 atagtttctt tgtgggggac gatgaaaagg taatcaagac cgtgcgcaag atagaacttg
     7801 ctgaggaata tcttgacgag gttcaggtcg acgatgttta ctatcttcac ggtgcctctt
     7861 cactcgcgga ccaaggggga gtatagcgaa aaaatatcgg catacaagag gaaaatgctg
     7921 ttttggcgaa gttttttttg caacggacct tttttccgga agaaaagtaa atgtttcatt
     7981 tactaaagaa attgctcacc gaaccataaa caattcttga caaagttttt ttgcggcgta
     8041 gtgtcgctcc cgttggtccc gatttttgaa ctctgagtac agaaaagagg cctaacaatg
     8101 cttggtatca aggaaatcgg catctacatt ccggtcggtc gtgagcccaa tcacgacaaa
     8161 aaagagaagt ggggcatcga cgacaccttc ctgcgcgaca agatcggcgt cgatcgccat
     8221 gccttccgtg accccggcga agaggcgacc gacatgggct acaaagcctt ccagcacctt
     8281 gcctcgaaga tcgacatcga ccgcacgaag gtcgaggtgc ttgtcttcgt cacgcagaat
     8341 cccgacgagc ggatgccgca cgccgggacg atactgcacg agaagatcgg tctgccctac
     8401 acctgtgcct cgttcgacat cccgctcggt tgctctggct tcatgtatgg tctgtcggtc
     8461 atcaccggct tcatgaagga gaacggtatg acgcgcggtc tcttgatcac cgccgatccc
     8521 tacaccaagt gcgtcaaccc cgaggacaag aacacctcgc tcctgttcgg cgacggcgcg
     8581 acggcgacct acatcaccga tgacccggtg cttgtgccgg gcaagtatgt cttcggcaat
     8641 ctcagcgaaa agtggcgcga gataaaactg gtcgacaact ggctctacat gaatggtccg
     8701 gcggtcgtga agttcgtagc caaatatgtg cccgagaatg tccgccggac gcttgagaag
     8761 aacaacctca agaaggaaga gatagaccgc tggatcatgc atcaggcgag ccgattcatg
     8821 cacaaggtca tcagcgagaa cctcgacatc agtcccgata agatgccgtg ggactgccgc
     8881 gactacggca acacggtgtc gagttcgata ccgctcctgc tcgagaagga actccacaac
     8941 aagcagcaca agcactactt catctgcggg ttcggcatcg ggctgtcgtg gggcagttcg
     9001 gtcctgagaa gggtctagta cagcagatat ttcttccgga tcttcttgaa tttcttgcag
     9061 tcggccgccg cctcggcgag tatcttcttc accgtctctc cgttcttgag ccgtttgtcg
     9121 atacccgcct tgcccatgct gatggcgaag tcggaggtcg ggtcgaagtc ggggaaaagg
     9181 gtggagagcg tccgcagcat cgcgagcccc gcggcgaaca gatcggcgga aaggtcattc
     9241 accgtcagcc gcacgccgcc gcactctttg ccgaagtggc ggtgcagttt atcggcgggg
     9301 gtgaaggtcg ccggcgcggc ggacactccg gcggggagcg cttttttcag cgcggcggcc
     9361 catttcgtcc cgtcgatgaa gggtgctccg tagaggaaga acggcgcttc ggtgccgcga
     9421 ccgaccgaaa tgttggtcgt ctctatccac cccatcccgg cgtagatgag cgccgcgtca
     9481 gggcttttca gatttggtga cggcgggacc cacggcagac cggtgtcctg ccagagcatc
     9541 gtccgcgtcc agccgctcat cgcgaccacc tcaagcgtcg cgccgatgcc gaagtggtcg
     9601 ttgaaaaggc gagccaactc tccgatggtc atcccgtggc gggtcgggat ggagtagaga
     9661 ctggtgaact tcccagtgag ttcgacggga gggacgaacc ccgcgacgac cgatccgccg
     9721 gcggggttcg ggcggtcgag aacgatcact ttctttccag ccgccttcgc cgcttcgagg
     9781 gtgtaggcga gcgtcgccgg ataggtgtag tagcgggcgc cgacatcctg cacatcgaac
     9841 acgacggcat cgacgtcggt cagcagttcg ggcttcggct tcttctcctt gccgtagagc
     9901 gacacgactg ggacattgcg gtaggtcgaa tcggcgaccg cttcgtcgga aagccccgtc
     9961 aggccgtgtt ccggggtgaa gatgcggacc accgttgcct ttttcccgtc gagcagaagg
    10021 tcgatgatat gtttcccgtc agcgttgcgt gcggtgtggt tgaccacgag cgccaccctt
    10081 tccccggcta tcgcgtcgaa gccgcgcgcc tcgagtacat cgagtcccac ctgcccccgc
    10141 gcctcgacaa ggagaggcag gagaagggcg aagaagaaga gatgcgttcg tgcaccaatg
    10201 gacatgcgca gaactccatg gttcgtggat cggcccattg taacaaaagt ttttccgcag
    10261 gcaacaacaa aaattctttt cttgcgagta tgggtgaaaa aggtaaggtg aagcgacaag
    10321 cggaggcggg atgccctact ttttcgtcac ccttctcata ttctcctcga tggaggtcgt
    10381 cagcaagccg ctgatgccgt tcgtcgaccc attccaactc accttccacc gttttcttgt
    10441 cggcctgatc tttctttttc tctttctcgt tgcgcgggga cggtgtcatg acctgatccg
    10501 catcggtcgg cacgatatgc ttctgctcgc ctttctcggg tttcttaaca cattcttcgc
    10561 gatgtcgatg cttcaggcgg cggtgaaaaa ttcgaccgcc gcgaccgccg cgacggtcat
    10621 ctgcgccaat cccctcttcg tctacctctt ttctctggta acgggagacg aatcgttctc
    10681 gtggcgccgc atcgccggca tcgttgccgg gatgataggc atcgcgctcg tcatgttcga
    10741 ccaggggctc cggtttgatg tcggcgtcct ttatgcgctc gccgccgccg ccgcctttgc
    10801 actgtacacc ctcctgaaca aaccggtagc tcgtcgtatc ggttcgcttc cggtgaatat
    10861 cgtctccttc tcgttcggta tcctcctctc cgcggtctac cttctcgtga cgggcgtcgg
    10921 ggtgcttccg cctctcgagg tgacgaacga gccggcacgt cttgccgcct ttctttacct
    10981 tggggtcgtg gtgagcggca tagggtatgt caccttcgtc aagaccatcc gcgcgctgac
    11041 cccggtcgcc gcttcggtca tcttcatcgt gaagccggcg ctcgccacgc tgttcgcggt
    11101 gatgttcctc ggcgagcgcc tctccgcgct gttctggccg ggacttctct ttgtcgtgct
    11161 gggaggcctg ctcatcctgc ggaacgcccc gtcgcaaaaa gttgacactc actaaaacga
    11221 cgataatctg tcaactaaag ttgcactcag ggaggaccct atgacccaag cgtccgatcc
    11281 ggcacaggta taccgccatc tggttcgtat actcggtgcg ctcgtgggga gcggaacgat
    11341 atcgcagttg gtctcctcgc tcgaaggagg actccgtgcc ttgaccccgc acctgttcct
    11401 cggcgtcgcg atatggaatc gttacgaaga cgaattggtc acctttctgc cgcgtggtcg
    11461 ccgcctcgaa cggggcctgc gccgctattt cgaagccata gaggcctcgc tgcacgatgc
    11521 cggcaccggt cgttatggtg agggtgcgct gttcgaacga ccggtccagc gggccgggca
    11581 ttcgttcttc tacgagcggc gcgcggtcga ggagggggtc accgcgatgc ttttcataga
    11641 gttcgtctcc ccggaggatg gcgattcctt cgggctcatc gccggtgacc ttgtcccgca
    11701 attttggggt cttctcgccg agcgttacgg taaagtggcg gccggcgagg agtccgacat
    11761 gctgtggcgg ctcaccgatg cggagaaacg ggaggaggac tggctcaacg agatcgaact
    11821 caaccaactc ctgcagaacc tgctacaact ctccctgcgc aaactcggcg cgaagatcgg
    11881 cgccatcctc ctgcttgacg aacggaccgg tgacttcatc atcgaaccgc gcgcggtggt
    11941 cggcaaggcg atatcggtca ttcccgagaa gttctcgacc aaggagcgct ccatcaccgc
    12001 catcgtgtat cgcaccaacc agtactacat ctgcaacgac accgaaagcg acggcaacta
    12061 ctatccgata ttccaaggca tcaagagctc gctcatcgtg ccgataacct ttcagggacg
    12121 gtgcatcggc gtcatcgcta tcgagagtca gcgcaaggga cggttctcgg tgagcgatgc
    12181 cgagaacctg caatcgatcg ccaagaccgc cacgatgttc atccgtcgcg cccaactgta
    12241 ccgtgagacg gcgcagagcg gtgacggcat catgatcttc gggcgcaccg agaagtggaa
    12301 agaggtcgaa cggcgcatcg agaaggcggc gcggaccgat gcgacggtca tcctgcgtgg
    12361 cgagagtggc accggcaagg aactcatcgc ccatgcgata catttcaaca gcccgcggcg
    12421 cgaccggccc ttcgtcgtca tcaactgcgc cgcgataccg tcggaactgc ttgaaagcga
    12481 aatgttcggt catgtgaagg gagccttcac cggcgcctac gccgacaaga ccggcgagtt
    12541 cgagcgggcc gacggcggca cgatattcct tgacgagatc ggtgatctgc cggtactcct
    12601 gcaggtgaag atactgcgca ccctgcagtc gggagagatc cgtccggtcg gctcctccaa
    12661 gccgccgcaa cgggtcaatg tccgcgtcat cgccgcgacg agccgcgatc tcgagcggat
    12721 gatagaggcg gggaccttcc gcctcgacat ctattaccgg ctccatgtcg tgccgatctg
    12781 gttgccgtcc ttacgcgagt accgtgagga catccccttc atcgtggagc atttcatccg
    12841 cgaggcgaac gaacgcttcc ataccacggt gcaacgaccg gacaaacggg cgcttgacct
    12901 gctcatggtc tacgactatc cgggcaatat gcgccagtta cggaattttg tacagcaggc
    12961 ggtcatcatg agcgacggcc cggtcctccg gtctgagcat ctgccgccgg aggtgcaggg
    13021 cgccgcgcct cttatctccg cctctgcggg gggatcgacc gctccccgtt tttccgccga
    13081 aggtccgttg ccctcttacc gcgaggagaa ggagcgggtg ctcgccgatt tttcgtcgga
    13141 atatctgcgg gcgctccttg cccggacgcg ggggaatatc aaacaggcgg cccgggaggc
    13201 gggcatatcc cgcatggcgc tttacaaatt gcttgcgcgc tatgccatac cgcaccatga
    13261 ctgagaaaac gattgcaaaa aaaagatatc ccgatacact ggatcacgca gaaaaagagg
    13321 gaggtgtgcg atgaaaagag ggcttgcggt cgtgatggta ctcgtgtcct gtctgttcct
    13381 cttctcctgt tcctcgtccg aagaggatga cccgaccggc gacgcggtcg tcatcgccga
    13441 cggcgatacg gtccccgacg gctcaacggt ggatggtggc gacgatctcg ccgggaccga
    13501 cgaggatacc gccgcgcagt gtaccccgaa cgagacggcc gaatgctacc acgggccgag
    13561 cggctcgcgc gaccgaggca tctgcaaggc gggactggcg acctgcggcg cgagcggcta
    13621 ctggggtccc tgcgagggag agatcttgcc ccagcccgag atatgccaca acgatatcga
    13681 cgagaactgt gacggtgagg acggtaccga agagaacacc gccgactacg atagcgacgg
    13741 gtatacttac tgcaccggcg actgctgcga gttcacctcg gaatgccagt tcccggcgct
    13801 cgtcaacccc gccgggtacg atttccccgg caacggcatc gacg
//
LOCUS       DAOQUE010000011        13757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_5025_length_13757_cov_4.354620, whole genome shotgun sequence.
ACCESSION   DAOQUE010000011 DAOQUE010000000
VERSION     DAOQUE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13757
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5025_length_13757_cov_4.354620"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PKH10_00725"
     CDS             complement(<1..420)
                     /locus_tag="PKH10_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26675.1"
                     /translation="MRRLALLTVLAVSITLSAQVVNPDWRPAEGPVTYAIPILDHATL
                     LPQELAIHYFTNLARMGYDCGDVALKCGLSETLPILSFDFKLATAARWFSKHLNDTPC
                     FQHDTCCTLTLTADEVLCVAGTACADQPCEVTFCAGTP"
     gene            complement(440..2263)
                     /locus_tag="PKH10_00730"
     CDS             complement(440..2263)
                     /locus_tag="PKH10_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26676.1"
                     /translation="MRAMWSMMFLLVTVTLSAQEAAPAAAENTSGSENSTPGQAVVEP
                     VVPPAPSPMTREEIEALVEKKVADRLAAIDEERKKEEERQQAEAKKKEEEAKKEAEKK
                     APPKSGVNDSPGLMTPSFSKNTLSFLVGDDNLRDNSQYSPETDIGPKDDYDNFMNRFL
                     GYSNRAKGMTRLSLFHEDGGLISDLTARIGIAFDLEHAFDTTYFVKTNLYEGGSFIEI
                     QYEKDFLIKVTAWPYDSDRMAVGFFPGLRWGTRAAWPQNLGFVPGAQLLFGWGPVSVY
                     AGFKAHLQPLVDKLSEETVPLETTYGGFGGVTYKDHGVTASLQGAFIDKGDNVMVDEA
                     ALRRSKDDEILSFGLDLFTQYEWKKPIGDPLGITSWNNGEWREPDYDGPLAARARGEL
                     IFLNELLQNADHLAWAGGEDAVTVEPITKNFWGMAAAVEGAFRWEKLRVFGLFSWRSL
                     SFLVFDAPGLIPFQTFPEHAKIRDELSGSLAVDYNWRMLWFGFSTGIKTPASYLAPGS
                     TRTTVVKDRLTANAKKVFTTRLREDLPDGANPLNIAFYAFTIKAQLTPAVAAAFQLSF
                     TKDSNRSKPVPDPDTGNNVLFWDDEKTRNILSFFVSLEGRF"
     gene            2424..4355
                     /locus_tag="PKH10_00735"
     CDS             2424..4355
                     /locus_tag="PKH10_00735"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNT26677.1"
                     /translation="MRRTVFLTLLSFLFFAACTPTDEGEPVVQVGEACSGTGTICSDV
                     AQDQLLRCDNGVLVIAEECGATGKACENGACVADEEVGGDCTGKEGRSACAGSKLVVC
                     TDNKWTESKDCATEDKLCVEEEGGAVCKDKPEEVDNTPPSDETVGEGDAIIQPDGEEE
                     TPDSDAEMQCTPFETAPCYHGPSNSDGVGICKSGLATCGASGYWGPCEGEVLPTAETC
                     NNGIDENCDGEDGTEANTDDYDGDGYTYCTGDCCEFDYECPVPAMTNPAAAEIKGNSV
                     DDNCDGKVDETISCDNGLTVSADTATSALALAKAAGMCDPWILSAELGLAGTPVTEQI
                     ADGPEGSSTISRPSLTKPYYDGSYKTYAVPPVFGQALVAKEGAALAVLSTGPWDSPTM
                     DAANATLQGGDMKTASTVPEDWINRQPNCEVPKAPSCGGADPDPNIQNSCAGKDPLAV
                     QDPIMLTVKLKVPINAVAFQMNTYFCSIEYPTTVCSSENYNDFFIALLDSTYNEVNPT
                     SSNPNPFDKNLAKDEAGNPVGVDLAPAGLFKICNPTCGGALSNTNPYGVCTGDLEIVG
                     TGFESQTIMGMCSGHGCTGWLLTAGNVVPGEEITLRFAVFEQGSVAYGPDHSWDSTIL
                     LDNFEWLTKETKPGTGAQE"
     gene            complement(4357..5367)
                     /locus_tag="PKH10_00740"
     CDS             complement(4357..5367)
                     /locus_tag="PKH10_00740"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HNT26678.1"
                     /translation="MQRTFSFIVLFLVVTLLSFAAAWQVQIAWGAEGISTKKEVKMDS
                     AKKVVKSEEEWKKELSPDQYRILRQAGTERAFTGALNDVYDPGLYRCGACGAPLFRSD
                     TKYDHGTGWPSFTAPIAEGAIEYRDDNTYGMSRVEVRCAACGSHLGHVFDDGPGPNGK
                     HYCINSASMKFDKATAVPAGPAKATFAAGCFWGVEFKLSQVPGVLSTRVGYTGGTTKD
                     PSYEEVCTDGTGHAEAIEVTYDPAKVSYEKLLDVFFDLHDPTQVDRQGPDIGTQYRSA
                     VFYHTAAQELAASAAIKKLNASGKFRRPIATQVAPVGEFYPAEEYHQKYYQKRGKQGC
                     GL"
     gene            complement(5475..7325)
                     /gene="typA"
                     /locus_tag="PKH10_00745"
     CDS             complement(5475..7325)
                     /gene="typA"
                     /locus_tag="PKH10_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNT26679.1"
                     /translation="MTVPARNEQIRNIAIIAHVDHGKTTLVDAMFRQSGVFREGQEVA
                     ERVMDSMDLERERGITIAAKNCAVHWNGVKINIIDTPGHADFGGEVERAISMADGALL
                     LVDASEGPLPQTRFVLKKALEAGLRVIVVINKIDRKDARPQEVLDEILDLFIDLEASD
                     DQLEFPLLYAIGRDGTAQKTLEEKGENLQVLFDLILKEIPAPAHDTAEPFQMLVSDLG
                     YSDYLGRLAIGKVINGSVRSNDQLVRIAEDGTQPPLKVTRLQTYDGIKVCPVDAADPG
                     DIIVLAGADEVMIGDTICNRESPKALPRIHVDEPTVSMRFAINSSPFAGKEGKIVQSS
                     KIRERLTKETLRNVSIQVEEGESRDSFIVKGRGEFQMSILIEQMRREGFELTVGRPEV
                     IYKYKEGRKQEPIEHVYIDCGEEFMGVVTEKLSIRRGRMVNLVNNGRGRVRMEFSAPS
                     RALIGFRDEFLTDTKGTGILNSLFEGYDDYRGDFPTRFTGSIVCDRQGEAVAYAIFNL
                     EPRGRLFVVPGDKVYEGMVVGEHNKDRDIDVNVCKEKKLSNMRAAGKDDNILLTPVRP
                     MTLEEAIHFIRDDELVEVTPLTIRLRKVTLDGLERHRSKGSELKAKREGE"
     gene            complement(7436..7852)
                     /locus_tag="PKH10_00750"
     CDS             complement(7436..7852)
                     /locus_tag="PKH10_00750"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNT26680.1"
                     /translation="MRSSFIAACVLLLTATAFAGWESGASSYDSIKARAKTAGTPYIV
                     LVRADWCPWCRKLDAFLDDNAIDALFRDKLAVKITPDNGDAEKRIAKDLGTKGFPSLF
                     VILPNGSTQKLSLPLKGTNKEMLDALRAQLDALYKK"
     gene            complement(7866..9173)
                     /locus_tag="PKH10_00755"
     CDS             complement(7866..9173)
                     /locus_tag="PKH10_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26681.1"
                     /translation="MRYALIALLVFANSALFAGTRSEAVLTGDSYVRAKWTCSAENVT
                     GTSCLSGIPNVYGSGNDYTGVAYDWGGSMSVATYLSSLAAGYRAGSCSGSSCSTYGVL
                     DCTVGVDCSGYVSMLWGCGHHTTSTIPDISTVIDQSAMKKGDVFNNAGSHVIMYIYTD
                     KSTGLPYIMESAGTGTEKTVFRRMSSWPSSSYERRRYDDIADDINVDGTIQNPRVISS
                     TPYYDEGNTRNVVSLEIDTYSALPGTKEFGPEVVYRIDLQSAGDLTATVTDNQAESID
                     CDVHLLSSLTIDGNGMAVDALVRDDHSVTRTLDAGTYYIVVDTYTGTTDLPGEYTLSV
                     DFVPSGTPTDDEPLPDEDTSVYDVVQADEDVAVSDEEMPDTEMPDQNGGDDTDEASVT
                     DNEVSADTDMLLPDEQNDTAGENPDDESLLEESSGCSCSIVQI"
     gene            complement(9170..9937)
                     /locus_tag="PKH10_00760"
     CDS             complement(9170..9937)
                     /locus_tag="PKH10_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26682.1"
                     /translation="MRKFLALWIVLAASLLCAGPVPIEFGSVPEKKLEVIKKIPYGTD
                     NGQVGIFIPGKAEEKPFGPNAISGDGTGLFILDNMNRRVLSLDIRTKILKKELSLPSA
                     TFDALFVSKGTLFLHNSKEERLYARSGDTVRPETKIAAGKTTVYPYTKFMGNGTARVV
                     VGEKEFFELAFTARSLMSFLLVGTADNGDLFFQIELADGSRHILITSGKGVVLGKMTL
                     PTSDLYKPEKDIHVAGDGTVYVIAPGADALAVYGGRL"
     gene            complement(9949..10443)
                     /locus_tag="PKH10_00765"
     CDS             complement(9949..10443)
                     /locus_tag="PKH10_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT26683.1"
                     /translation="MTAPLTFRQAVPADFDELWPILHEVFESGESYPHAPGTTKEEGY
                     RYWIETPTVSIVAILDGRIAGTYYLRPNQVGLGSHVANAGYMVASWARGKGIGRALCL
                     NSIEEAPKHGFKALQFNLVVASNAPAVHLWTSCGFTIIGTIPKAFDHKRLGLVDAYIM
                     HRFV"
     gene            10660..11865
                     /locus_tag="PKH10_00770"
     CDS             10660..11865
                     /locus_tag="PKH10_00770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNT26684.1"
                     /translation="MAKRPIKVALFSEVLKEHLDGVTNTLYHIIDRIPKDRFEYLFIT
                     SYPPSETVNLPFPVITVPKIAFPTNPDYPISLPFFNGKLRAALDEFAPDIIHLTTPFT
                     LGNYALSYGEERGVPILATYHTHFISYIEYYFRYAPIFSHLLDFSGRRYMRWFYNRCD
                     LTYVPTASIMDELLDCGIERRRMVVWSRGIDTELFNPTRRDDAFMDELCGAGTQRVLF
                     VSRLVWEKELRTLAAVYELFRETHPRTVRFVVTGDGPRRKELERLMPDAVFTGKLVKK
                     DLAKVYASSDVFLFPSITETFGNVVLEAMASGLPPVVAAQGGPKGIVRDGDTGLHARP
                     KDAADFHRKIALLLDDPALAKETGRRAAAYALAQQWDTLCEDMFSSYERISERFAPLS
                     PEKAHGVAV"
     gene            11849..12919
                     /locus_tag="PKH10_00775"
     CDS             11849..12919
                     /locus_tag="PKH10_00775"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNT26685.1"
                     /translation="MVSLYRPAVKTILARLFKIAALTAGFFLVWWFVSASDIGRITDQ
                     LGHVGYRFMLLLLVTFCAQLLITRALQLSFIDTPPLSLLRLFSIRLMGESLAQVNPTS
                     FVAGETMKAVMLSRRGVPYNRSISALTIARLTLWLSSITLFGLGALLFLDRLDFAGNR
                     VLMTLLAGGLLTGGLFFVRQISRSLPFFSLFAMILRRLLPWVRTFIIVERKLHKVDGD
                     LSFFYHYRRREFARAYALSLLHWLGGATEFFLILWFLGVEVSFFSCIAMEVGVMLFKG
                     LGTFVPGQIGIEEYANRLMLDLVGVPGSELWVSVSILRRTRQLFWIGMGTLLFSVTMM
                     RRRRTAPLPTSVETYGNPVHHP"
     gene            12942..>13757
                     /locus_tag="PKH10_00780"
     CDS             12942..>13757
                     /locus_tag="PKH10_00780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT26686.1"
                     /translation="MQKQSFELIRGLSRRMTVHTLIHSGDSGKGLFFLTLKKRVLQML
                     REHPGISLIHLNDGLMAFFALPLRSVTDLPIVATLHGLDVVMPAGWYQRRVLPRFAAY
                     DGIIAVSSATAQACRQRGFDHAKITVVPNGVDTALGDIPAEPNYRAVIGRRLGLDLSH
                     RKLVLMLGRPVPRKGFIWFLREVLPKLGPEVVCLHIGPSAAAMGLKNFLLRLLPFNWG
                     RQLALIFGMPTDEPALRHELALPELAGRAFRLGGLPFKDLVQTIKCGDLYVMPN"
BASE COUNT         2441 a         4366 c         4174 g         2776 t
ORIGIN      
        1 cggggtgccg gcgcagaagg tgacctcgca cggctggtcg gcgcaggcgg tccccgcgac
       61 gcagagcacc tcgtcggcgg tcagcgtgag cgtacagcag gtgtcgtgct ggaaacaggg
      121 ggtatcgttc aggtgcttgc tgaaccagcg ggccgccgtc gcgagtttga agtcgaacga
      181 cagtatcggc agcgtctcgc tcaggccgca cttgagcgcg acatcaccgc agtcgtaccc
      241 catccgggcg aggttcgtga agtagtgtat cgccagttcc tgcggcagga gcgtcgcgtg
      301 gtcgagtatc gggatggcat aggtcaccgg cccctcggcg ggccgccagt cggggttcac
      361 cacctgcgcc gacagcgtga tgctcacggc gaggaccgtc agtaacgcaa gtcgtctcat
      421 tctctcctcc cctgcggtat cagaatcttc cctcgagact gacgaagaac gacaggatgt
      481 tgcgggtctt ctcgtcatcc cagaacagca cattgttccc cgtatcggga tcgggcaccg
      541 gcttggaacg gttggagtcc ttggtgaagg agagttggaa cgcggcggcg accgccgggg
      601 tcagttgcgc cttgatcgtg aaggcgtaga aggcgatatt gagcgggttc gccccgtcgg
      661 gcaggtcctc gcgtagccgg gtcgtgaaga cctttttggc gttcgccgtc agacggtcct
      721 tgaccaccgt cgtccgggtg cttcccggcg caaggtagga ggctggggtc ttgatgccgg
      781 tcgagaagcc gaaccagagc atccgccagt tgtaatcgac cgcgagcgag ccggacagtt
      841 cgtcgcggat cttcgcatgc tccgggaagg tctggaacgg gatgagcccc ggcgcgtcga
      901 acacgaggaa cgagagcgac cgccacgaga acaggccgaa cacgcggagt ttctcccagc
      961 ggaacgcccc ctcgaccgcc gccgccatcc cccagaaatt cttcgtgatc ggctcgaccg
     1021 tcacggcgtc ctcaccgccc gcccacgcga ggtggtcggc gttctgcagc agttcattga
     1081 ggaatatcag ttcgccgcgc gcccgggccg ccagcgggcc gtcgtagtcg ggctcgcgcc
     1141 attcgccgtt gttccacgag gtgatgccga gcgggtcgcc gatcggcttt ttccattcgt
     1201 actgcgtgaa caggtcgagc ccgaaggaaa gtatctcgtc gtccttcgac ctccggagcg
     1261 ccgcctcgtc caccatcaca ttgtcgccct tgtcgatgaa ggcgccctgt agactcgcgg
     1321 tgacgccgtg gtccttgtag gtcaccccgc cgaagccgcc gtaggtcgtt tcgagcggca
     1381 ccgtctcctc gctcagtttg tccacgagcg gttggagatg cgccttgaac ccggcgtaga
     1441 ccgagaccgg accccagccg aagagcagtt gcgcgccggg gacgaacccg aggttctgcg
     1501 gccacgcggc gcgggtcccc cagcgaagcc cggggaagaa gccgaccgcc atccggtcgg
     1561 aatcgtaggg ccacgcggtg acctttatca ggaagtcctt ttcgtattgt atctctatga
     1621 aagagccgcc ctcgtacagg ttcgtcttga cgaaataggt cgtgtcgaag gcgtgctcga
     1681 gatcgaacgc gatgccgata cgggcggtga ggtccgatat caacccgccg tcctcgtgga
     1741 acagcgacag gcgcgtcatt cctttggcgc ggttgctgta gccgaggaag cggttcatga
     1801 agttgtcgta gtcgtccttg gggccgatgt cggtctcggg cgaatactgc gagttgtcgc
     1861 gcaggttatc gtcacccacg aggaatgaaa gggtgttctt ggagaaactc ggcgtcatga
     1921 gcccggggga gtcgttcacg ccgcttttgg gcggggcctt cttctccgcc tccttctttg
     1981 cctcctcctc tttctttttc gcctcggcct gctgccgttc ttcttccttt ttccgttcct
     2041 catcgatggc ggcgagccgg tcggcgacct ttttctcgac aagcgcctct atctcctcgc
     2101 gggtcatcgg ggaaggagcg gggggcacga ccggctcaac gacggcttgt cccggcgtag
     2161 agttttcgga gccggaagtg ttttcggcgg cggcgggcgc cgcctcctgc gccgacagcg
     2221 tcaccgttac cagcaggaac atcatcgacc acatcgctcg catacacact ccccatgtct
     2281 cgttcagacc gggcgcatca tatcgtcctt cgcggcaata ggcaattttt agcgcggcga
     2341 ccctcgtgaa aaaaatggtt gcaaagcatc cgcgtcccgc tataatcccc cttaccataa
     2401 cagtggcatg gaggaacagc gcgatgcgac ggaccgtctt tctgaccctt ctttcctttt
     2461 tattctttgc cgcctgcacc ccgaccgacg agggtgagcc ggtcgtacag gtcggcgagg
     2521 cctgttccgg gaccgggacg atctgttccg atgtggcgca ggaccagttg ctccgctgcg
     2581 ataacggcgt gctcgtgata gccgaggaat gtggcgcgac cggcaaggcg tgcgagaacg
     2641 gcgcctgtgt cgccgacgaa gaggtcggcg gggattgtac cggtaaggag gggcggtcgg
     2701 cctgcgcggg gagcaaactc gtggtctgca ccgataacaa atggaccgaa tcgaaagact
     2761 gcgcgaccga ggacaaactg tgcgtcgaag aggaaggcgg cgcggtctgc aaggacaaac
     2821 ccgaggaggt ggacaacacg ccgccgagcg atgagaccgt cggcgaaggc gacgcgatca
     2881 tccagccgga cggcgaggaa gagacgcccg acagcgatgc ggagatgcag tgcaccccgt
     2941 tcgaaacggc cccgtgctac cacggaccga gcaattccga cggcgtcggc atctgcaaat
     3001 ccggccttgc cacctgcggc gcgagcggct actggggccc ctgcgagggg gaggtgctcc
     3061 cgaccgccga gacctgcaac aacggcatcg acgagaactg cgacggcgag gacggcaccg
     3121 aggccaacac tgacgattac gacggtgacg gctacaccta ctgcaccggc gactgttgcg
     3181 agttcgatta cgaatgtccg gtgcccgcga tgaccaaccc cgccgccgcc gagataaagg
     3241 gcaacagcgt ggacgacaac tgtgacggca aggtggacga gaccatcagt tgcgataacg
     3301 gcctgacggt gagcgccgac accgcgacga gcgccctcgc gctcgccaag gcggccggca
     3361 tgtgcgaccc gtggatactt tcggccgaac tcgggctcgc cgggacgccg gtcaccgaac
     3421 agatcgccga cggccccgag ggtagttcga ccatcagccg cccgtcgctg accaaacctt
     3481 actatgacgg cagttacaag acctatgcgg tgccgccggt cttcggacag gcgcttgtcg
     3541 cgaaggaggg cgccgcgctc gccgtccttt cgaccgggcc gtgggacagc ccgaccatgg
     3601 acgccgccaa tgcgacgctg caaggcggcg acatgaagac cgcctcgacg gtccccgagg
     3661 attggatcaa ccgccagccc aactgcgagg tccccaaggc cccctcgtgc ggcggcgccg
     3721 atcccgaccc gaacatccag aactcctgcg ccggcaagga tccgctcgcc gtgcaggacc
     3781 cgatcatgct cacggtgaaa ctcaaggtgc cgataaacgc ggtcgccttc cagatgaaca
     3841 cctacttctg ctcgatagaa tacccgacga ccgtctgctc ctccgagaac tacaacgact
     3901 tcttcatcgc gctcctcgat tcgacctaca acgaggtgaa cccgacctcc agcaacccca
     3961 acccgttcga caagaatctc gccaaggacg aagcgggcaa tccggtcggg gtcgatctcg
     4021 cccccgccgg cctgttcaag atatgcaacc cgacctgcgg cggtgcgctt tccaacacga
     4081 acccctacgg cgtctgcacc ggcgacctcg agatcgtggg gaccggcttc gagtcacaga
     4141 ccatcatggg gatgtgctcg ggccacgggt gcaccgggtg gctcctgacc gcgggtaatg
     4201 tcgtgccggg cgaggagata acgctccgtt tcgcggtctt cgaacagggt tccgttgctt
     4261 acggacccga tcattcgtgg gattcgacga tactcctcga taacttcgag tggctcacca
     4321 aagagaccaa gccgggcacc ggcgcacagg aataggtcag agcccgcacc cctgcttgcc
     4381 ccgtttctgg tagtacttct ggtgatactc ctcggcgggg tagaactctc cgacgggggc
     4441 gacctgcgtc gcgatcggac ggcggaattt gcctgatgcg ttgagttttt tgatggcggc
     4501 gctggccgcc agttcctgcg cggcggtgtg gtaaaaaacg gccgatcggt actgcgtccc
     4561 aatatcgggc ccctgccgat ccacctgcgt cgggtcatgc aggtcgaaga aaacatcgag
     4621 cagtttctcg taactcacct ttgccgggtc gtaggtcacc tcgatcgcct cggcgtggcc
     4681 cgttccgtcg gtgcagacct cctcgtaact cgggtccttc gtcgtgccgc cggtgtagcc
     4741 gacgcgggtc gataacaccc ccggcacctg cgacagtttg aactcgacac cccagaaaca
     4801 gcccgccgca aaggtcgcct tggccggtcc cgcaggaacg gcggtcgctt tgtcgaactt
     4861 catcgacgcc gagttgatgc agtaatgttt gccgttcggt ccgggtccgt catcgaatac
     4921 atgaccgagg tgcgaaccgc aggcggcgca acggacctcg acgcggctca tgccgtaggt
     4981 gttatcgtcg cggtattcga tcgccccttc ggctatcggt gcggtgaacg agggccaacc
     5041 ggtgccgtga tcgtacttcg tgtcgctccg gaacagcggc gcgccgcagg cgccacagcg
     5101 gtagaggccc ggatcgtaga catcgttgag cgcgccggtg aaggcccgct cggtccccgc
     5161 ctgccgcagg atacggtact gatccggaga gagttccttc ttccactcct cttcgctttt
     5221 caccaccttt ttggcgctat ccatcttcac ctcctttttt gttgaaatgc cttccgcgcc
     5281 ccacgcgatc tgcacctgcc acgccgccgc gaacgacagg agcgtcacca cgaggaacag
     5341 cacgatgaat gagaatgttc gttgcatggt acacctccgt accttcatta tttctctatt
     5401 ataatcagag cgttgctacc ggcaacagaa aaggtgtcaa gtgtccggaa ttgataggga
     5461 acgggcgcgg ggaactactc cccttcgcgc tttgccttga gttcggaacc tttggagcgg
     5521 tgccgctcca agccgtcgag ggtgaccttg cgcagacgga tggtgagggg tgtcacctcg
     5581 accagttcgt cgtcgcggat gaagtgtatc gcctcctcga gcgtcatcgg ccggaccggg
     5641 gtgaggagga tgttgtcgtc cttgcccgcc gcccgcatat tggaaagttt cttttccttg
     5701 cacacattca catcgatgtc gcggtccttg ttgtgttcgc cgaccaccat accctcgtag
     5761 accttgtcgc ccggcacgac gaagagccgt ccgcgcggtt cgaggttgaa tatggcgtag
     5821 gcgaccgcct caccctgccg gtcgcagacg atcgagccgg tgaagcgggt cgggaaatcg
     5881 ccgcggtagt cgtcatagcc ctcgaagagc gagttgagga tgccggtccc cttcgtgtct
     5941 gtcaggaact cgtcgcggaa cccgatgagt gcgcgtgacg gcgccgagaa ctccatccgg
     6001 acccggcccc ggccgttgtt gacgaggttc accatccggc cgcgccgtat cgagagtttc
     6061 tcggtcacga cccccatgaa ctcctcgccg cagtcgatgt agacatgctc gatcggttcc
     6121 tgctttctgc cctccttgta tttgtagatg acctcggggc ggccgacggt cagttcgaac
     6181 ccctcgcggc gcatctgctc gatgaggatg gacatctgga attcgccgcg tcccttcacg
     6241 atgaaactgt cgcggctctc cccttcttcg acctgtatcg aaacattgcg gagtgtttcc
     6301 ttggtcagcc gttcgcgtat cttcgacgac tggacgatct ttccctcctt cccggcaaaa
     6361 ggcgacgaat tgatcgcgaa gcgcatcgac actgtcggct cgtcgacatg gatgcgcggt
     6421 agcgccttgg ggctctcacg gttgcagatg gtgtcgccga tcatcacctc gtcggcgccg
     6481 gcgagcacga tgatgtcgcc cgggtcggcc gcatcgaccg gacagacctt gatgccgtcg
     6541 taggtctgga ggcgggtcac cttgagcggc ggctgggtgc catcctcggc gatgcggacc
     6601 agttgatcgt tcgacctcac cgatccgttg atcaccttgc cgatggcgag tcgaccgagg
     6661 taatcggagt agccgaggtc cgaaacgagc atctggaacg gctcggcggt gtcgtgggcg
     6721 ggggccggta tctccttcag tatcaggtcg aacagcacct gcaggttctc ccctttctcc
     6781 tcgagcgtct tctgcgcggt gccgtcgcga ccgatggcgt agaggagagg gaattcgagt
     6841 tggtcgtcgc tcgcttcaag gtcgatgaag aggtcgagta tctcgtccaa gacctcctgc
     6901 ggtcgggcat ccttgcggtc gatcttgttg atgaccacga tgacccgcag tcccgcttcg
     6961 agcgcctttt tgaggacgaa ccgcgtctgc ggcagcggcc cttccgacgc gtcgacgagg
     7021 agtagcgcgc cgtcggccat cgagatagct cgttcgacct cgccgccaaa atcggcgtgg
     7081 ccgggggtgt cgatgatgtt tatcttgacg ccgttccagt ggacggcgca gttcttcgcc
     7141 gctatcgtga tgccgcgttc ccgctcgaga tccatgctgt ccatgacccg ctcggccact
     7201 tcctgacctt cgcggaagac gccgctctgg cggaacatgg cgtcgacgag cgtcgttttg
     7261 ccgtggtcga catgggcgat gatggcgata ttgcgtatct gttcgttacg ggcgggcacg
     7321 gtcataaaaa ctccttatcg aacgatcggc ccgctttata gtcagagatt cgcgatttgc
     7381 aatagaaaaa gttacttcgg gtactgcacg aagccgagtt cgaggccgtg cggtgttatt
     7441 tcttgtacag cgcgtcgagt tgggcgcgga gcgcatcgag catctccttg ttcgttcctt
     7501 tgagcgggag cgacagtttc tgcgtcgaac cgttcggcag gatgacgaac agcgacggga
     7561 acccctttgt ccccagatcc ttcgctatcc tcttctcggc gtcgccgttg tcgggcgtta
     7621 tcttcaccgc cagtttgtcg cggaagaggg cgtcgatggc gttgtcgtcg aggaacgcgt
     7681 cgagtttccg gcaccacggg caccagtcgg cgcggaccag cacgatgtag ggcgttcccg
     7741 ccgtcttggc tcgcgcctta atggagtcat aactgctggc gccgctctcc cacccggcga
     7801 aggcggtcgc ggtcagcaac agcacgcagg cggcgatgaa tgaacttctc atgatatcct
     7861 ccttatcata tctgaactat agagcaggag caaccggaac tttcttcgag cagagactcg
     7921 tcatcggggt tctctccggc ggtgtcgttc tgttcatccg gcaggagcat atcggtatcg
     7981 gccgagacct cattgtcggt gacactcgct tcatcggtat cgtcgccgcc gttctggtcc
     8041 ggcatttcgg tatcgggcat ctcttcgtcg ctgacggcga catcctcgtc ggcctgcacc
     8101 acatcataga cgctggtgtc ctcgtcggga agcggctcgt cgtcggtcgg tgtcccggac
     8161 ggtacgaaat cgacactcaa ggtgtattcg cccgggagat cggtggtacc ggtataggtg
     8221 tcgacgacga tatagtaggt accggcatcg agcgtccgcg tgacgctgtg atcgtcccgt
     8281 acgagggcgt ccaccgccat gccgttcccg tcgatggtga gcgaagagag cagatggacg
     8341 tcgcaatcga tgctctcggc ctggttgtcg gtcaccgtcg cggtcaggtc gcccgcgctc
     8401 tgcaggtcga tccggtagac gacctcggga ccgaattcct tcgtgccggg gagggccgaa
     8461 taggtgtcta tctcaaggga gaccacattc cgggtgttcc cctcatcgta atagggtgtc
     8521 gaggagatca cgcgggggtt ctggatggtc ccgtcgacgt tgatgtcgtc cgcgatatcg
     8581 tcgtagcgcc gtctttcata actgctggag ggccaactgg acatcctgcg gaacaccgtc
     8641 ttctcggtcc cggtccccgc gctctccatg atgtagggca gaccggttga tttgtcggtg
     8701 tagatataca tgatgacatg actgccggcg ttgttgaaga catcgccttt cttcatggcg
     8761 ctctggtcga tgacggtcga gatatcggga atggtgctgg tcgtatgatg accgcatccc
     8821 cacagcatcg agacatagcc ggagcagtcg acgccgacgg tgcagtcgag gacaccgtat
     8881 gtcgaacagg aactgccgct gcaggagccg gcgcggtagc ccgcggcgag gcttgacaga
     8941 taggtcgcca cgctcatcga gccgccccag tcgtaggcga cgccggtgta gtcgttcccg
     9001 ctgccgtaca cattcgggat gcccgacagg caggaggtcc cggtcacatt ctccgccgaa
     9061 caggtccact tcgcgcggac ataactgtcg ccggtaagga ccgcttcgct tctcgtgcca
     9121 gcgaaaagag cgctgttcgc aaaaacaagc agggcgatca gggcgtatct catagccgcc
     9181 ccccgtagac ggcaagcgca tcggcgccgg gagcaatgac atagaccgtc ccgtcgcccg
     9241 ccacatggat gtctttttcc ggtttataca gatcggatgt tggcagggtc atcttgccaa
     9301 gaacgacacc cttccccgaa gtgatgagga tatggcggct gccgtccgcc agttcgatct
     9361 ggaaaaagag atcgccgttg tcggccgtgc cgaccagcag gaacgacatg agcgaacggg
     9421 cggtgaaagc gagctcgaag aactccttct cgccgacgac cacgcgggcc gtgccattcc
     9481 ccatgaattt cgtgtaggga tagaccgtcg tttttcccgc cgcgatcttc gtttccggcc
     9541 tcaccgtatc gccgcttctc gcatacagtc gttcctcctt gctgttatgg aggaaaagcg
     9601 tccccttgct cacgaacagc gcatcgaatg ttgcggaggg gagagaaagt tccttcttca
     9661 ggatctttgt ccggatatcc agcgaaagga cccgccggtt catattgtcc agaatgaaaa
     9721 gccccgtccc gtcgccggag atcgcgttcg gcccgaacgg tttctcctcc gcttttcccg
     9781 gaatgaagat gcccacctga ccgttgtcgg tcccgtacgg gatctttttg atcacctcca
     9841 gtttcttctc cggcaccgat ccgaactcga tcggcaccgg ccccgcgcaa agaagcgaag
     9901 cggcaagaac gatccacagt gctaagaact tcctcatatc tccctccctc acacgaaccg
     9961 gtgcatgatg taggcgtcca cgagtccgag ccgtttatgg tcgaacgcct tgggtatcgt
    10021 accgatgatg gtgaagccgc acgaggtcca cagatggacc gccggggcgt tcgatgcgac
    10081 gacgaggttg aactggagcg ctttgaagcc gtgtttcggc gcctcctcga tgctgttgag
    10141 acacagcgcc cggccgatcc ccttgccccg cgcccatgag gcaaccatgt agccggcgtt
    10201 ggcgacatgc gatccgaggc cgacctgatt ggggcgcagg tagtaggtcc cggcgatgcg
    10261 gccgtcaagg atcgcgacga tcgatacggt cggtgtctct atccagtagc ggtagccctc
    10321 ttccttcgtc gtgccgggcg catgcggata actctcgccg ctctcgaaca cctcgtgcag
    10381 gatgggccac aactcgtcga aatcggccgg cacggcctga cggaaggtga gcggtgcggt
    10441 catggttctc tcgcatgcgg atctccccca ctctaccaac tctctcaaga aaaacaacgg
    10501 ccgtggtagt aaaaaatttt tacgagctcc gatgctccgt aaaggaactt tccgagcgcc
    10561 gtcttgtttt tcgctagtta tttcaatgaa ttggtagagg cacgcctctt gcttatatgc
    10621 gcgccctttt gttaaggaag agagaggtcc cggtacgcga tggcgaaacg gccgataaag
    10681 gtggcgctct tcagcgaggt gctcaaggag catctcgacg gcgtgaccaa cacactctat
    10741 cacatcatcg accgtatccc gaaggaccgg ttcgagtatc tgttcatcac gagttacccg
    10801 ccgagcgaaa cggtgaacct ccccttcccg gtcatcaccg taccgaagat agcgttcccg
    10861 accaatcccg actatcccat ctcgctcccc ttcttcaacg ggaaactgcg ggcggcgctc
    10921 gacgagttcg cccccgacat cattcacctt acgaccccct tcacgctcgg caactacgcg
    10981 ctctcgtacg gcgaggagcg cggcgttccg atccttgcca cctatcacac ccacttcatc
    11041 agttacatcg aatactattt ccgttacgcc ccgatattct cgcacctgct cgatttctcg
    11101 ggccgccgtt atatgcgctg gttctacaac cggtgcgacc tcacctatgt gccgaccgca
    11161 agcatcatgg acgaactgct cgactgcggc atcgagcgac ggcgcatggt ggtgtggagc
    11221 cgtggtatag acaccgaact gttcaacccg acgcggcgcg acgacgcctt tatggacgaa
    11281 ctgtgcggcg cggggaccca gcgggtgctc ttcgtgagcc ggctggtgtg ggaaaaggag
    11341 ttacggacgc tcgcggcggt gtacgaactg ttccgcgaga cccacccgcg gacggtccgg
    11401 ttcgtcgtca ccggcgacgg gccgcgccgc aaggaactcg aacggctcat gcccgatgcg
    11461 gtcttcaccg gcaaactggt caagaaggac ctcgccaagg tctatgcctc gagcgatgtc
    11521 ttcctttttc catccatcac cgagaccttc ggtaatgtgg tgctcgaggc gatggcctcg
    11581 ggcctgcccc cggtcgtcgc ggcgcagggc ggcccgaaag ggatcgtgcg cgacggcgac
    11641 accggtctgc acgcgcggcc gaaggacgcg gccgatttcc accgcaagat agcgctcctc
    11701 ctcgacgacc ccgctctggc caaggaaacg ggacggcgcg ccgccgccta cgcgctcgct
    11761 cagcagtggg acacgctgtg cgaggacatg ttctcttcct acgagcggat atccgaacgg
    11821 ttcgcccctc tttcaccgga gaaggcccat ggtgtcgctg tatagaccgg cggtcaagac
    11881 catacttgcg cggctgttca agatagcggc gctcaccgcg gggttcttcc tcgtctggtg
    11941 gttcgtgagc gcctccgaca tcggtcgcat caccgatcag ctggggcatg tcggctacag
    12001 attcatgttg ctcctgctcg tgaccttctg cgcgcaactg ctcatcacgc gggcactgca
    12061 actcagcttc atcgacaccc cgcctctgtc gctcctgcgc cttttctcga tacggctcat
    12121 gggcgaatcg ctcgcgcagg tgaaccccac cagtttcgtc gcgggcgaga cgatgaaggc
    12181 ggtcatgctc tcgcggcgcg gcgtcccgta caaccgcagc atctcggcgc tcaccatcgc
    12241 gcggctcacg ctgtggcttt cgagcatcac gctgttcggg ctcggcgccc tgctgttcct
    12301 cgaccggctc gatttcgccg gcaaccgggt gctcatgacg ctcctcgcgg gaggcctgct
    12361 caccggcggg ctcttcttcg tccgccagat atcgcggtcg cttcccttct tctcgctgtt
    12421 cgcgatgatc ctgcgccgcc tgttgccatg ggtgcgcacc ttcatcatcg tcgaacgaaa
    12481 actgcacaag gtggacggcg atctctcgtt cttctaccac taccgccgcc gcgagttcgc
    12541 gcgggcctac gccctgtcgc tcctccactg gctcggcggc gcgaccgagt tcttcctcat
    12601 cctgtggttc ctcggcgtcg aggtgagttt cttctcctgc atcgcgatgg aggtcggcgt
    12661 catgctcttc aaggggctcg gcaccttcgt cccgggccag ataggcatcg aggagtacgc
    12721 gaaccggctc atgctcgatc tcgtcggcgt gccgggctcc gaactctggg tcagcgtctc
    12781 gatactgcgc cgcacccgcc aactgttctg gatcggcatg gggacgctcc tgttctccgt
    12841 caccatgatg cgccgccgcc ggaccgcccc gctccccacc tcggtcgaga cctatggaaa
    12901 tcctgtgcat cacccataag tatcccccct cggtcggggg catgcagaaa cagagtttcg
    12961 aactcatccg cggcctgtct cgccgcatga cggtccatac gctcatccac agcggcgaca
    13021 gcgggaaagg tctttttttc ctgacgctta agaaaagggt cctgcagatg ctccgcgaac
    13081 atccgggcat ctcgctcatc catctcaatg acgggctcat ggctttcttc gccctgccgc
    13141 tccggtcggt gaccgatctt cccatcgtcg cgacgctcca cgggctcgat gtggtgatgc
    13201 cggcgggctg gtaccagcgg cgggtcctgc cccgtttcgc cgcttacgac ggcatcatcg
    13261 cggtgagttc cgctaccgcg caggcctgcc gccagcgcgg gttcgaccac gccaagataa
    13321 cggtcgttcc caacggcgtc gacacggcgc tcggtgacat accggccgaa ccgaactacc
    13381 gcgccgtgat cgggcgacgg ctcgggctcg acctctcgca tcgcaaactc gtgctcatgc
    13441 tcggccgccc ggtgccgcgc aagggcttca tctggttcct gcgcgaggtc ctgccgaaac
    13501 tcgggcccga ggtcgtgtgt ctccacatcg gtccctcggc ggccgcgatg ggtctcaaga
    13561 atttcctgct ccgcctgctc cccttcaatt gggggcggca actggcgctc atattcggga
    13621 tgccgaccga cgaaccggcg ctccgtcacg aactcgccct gcccgaactc gctggccgcg
    13681 ccttccggct cggcgggctc cccttcaagg acctcgtgca aacgatcaag tgcggcgatc
    13741 tctatgtgat gcccaat
//
LOCUS       DAOQUE010000012        13694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_5051_length_13694_cov_4.524599, whole genome shotgun sequence.
ACCESSION   DAOQUE010000012 DAOQUE010000000
VERSION     DAOQUE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5051_length_13694_cov_4.524599"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            418..2082
                     /locus_tag="PKH10_00785"
     CDS             418..2082
                     /locus_tag="PKH10_00785"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019390139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNT26687.1"
                     /translation="MPTDLDIASQVSLRPIADIAAKLGLRREHLEPYGDHIAKVRPTA
                     RALGEPQAKQVLVTAMTPTPLGEGKTTVAIGLSEALNRIGKRSIVTLREPSLGPVFGV
                     KGGAAGGGWSQVLPMTDINLHFTGDIHAVTTAHNLLAALIDNQYSRVTQQCLLPREIV
                     WHRVMDMNDRSLRNIVIGLGKESGQVRESKFEITASSEVMAILALAYDLDDLKKRLGD
                     IIVAVKPKGEPLHAHDLNAHGAMAILLANAIKPNLVQTIEGNPAFIHAGPFANIAHGT
                     NSIIATDLARRLSDIVVTEAGFASDLGGEKFFNLVSRQKGMTPPDAVVVVATVRALKY
                     HGGVVRKDLGRPDPGAVERGFPNLEKHIGNMRSFDRPVLVALNRFPGDSDDEIAAARR
                     LCERHGAVCFPVEVWAKGGAGATELAAAVWGAAHDAHGPVRYTYELDDPAAVKIEKVA
                     KAMYGAEGVHIPRSVLAKIEERESWGYRGLPVCMAKTQSSLSDTPDLRGRPTGFTVEI
                     RDVKVSAGAGFMVAYTSDIMTMPGLPETPASDRMDIDSDGAISGLF"
     gene            2317..3339
                     /locus_tag="PKH10_00790"
     CDS             2317..3339
                     /locus_tag="PKH10_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HNT26688.1"
                     /translation="MAGQPIIQPQGEIVIYQNPDGNIRLDVRLEEETVWLTQAHMAIL
                     FDKAKSTINEHIQNIFEEGELVEGQVMRKFGNPEFSTKPTNYYNLDVIISVGYRVKSP
                     QGTQFRIWATKRLKEYIVKGFALNDERFKTGNSMNYFNELQDRIRQIRLSERFFYQKI
                     KDIYATSIDYDPQDERTIEFFKVVQNKLLWAINQQTAAELIYRRVDATLPLMGMRSFD
                     KAGIAKIKKSDIAIAKNYLTEEEMKLLGLLVEQYLAFAETMAQQHKPMYMSDWIARLD
                     EILRMNGRELLTHAGKISHETAIQKSETEYEKYRKNEQRAEKEASLAEIETDIKQLKQ
                     ITKKGK"
     gene            3428..6787
                     /locus_tag="PKH10_00795"
     CDS             3428..6787
                     /locus_tag="PKH10_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNT26689.1"
                     /translation="MFITNEKTNTLEKRLNELVPISRELKFLVGFFYFSGIRGIYSSL
                     KNNPEATLKVLVGLDTDATIPKMVVEVPKNDETMSNERSRSCYLESIKTGMRQKDFDS
                     EECYEQISFFLDLLKEGRLQIKKTLDPNHAKLYIFNFKESYSQLRSQLFISGSSNLTR
                     AGQVGQGELNVEISDFGLTKAAEDHFNKLWETAIKITEHEPTRDRLIKIIAEQTLISR
                     LSPFEAYLLVLKTYLEVLTATNHTVAFSQSLEEKGYREFRYQNDAVAQALDIIEENGG
                     VVIADVTGLGKSVIASRIAHEMRERGIIICPPALIGDEARTVGWKKYCKDFDLLNWDI
                     FSRGNLEGAFECVANDPSIKVVIVDEAHHFRNEGIYSYEQLKAICRGRKVILLTATPF
                     NNTPADLFALLKLFVVPGKSVLTLDEDLEGRFRSYRNLYAKLSYILKYYNSKKPEKRI
                     TAENYYQRLFGSLPVDVRLVREETRKLSQRIKDTIRPAVIRRNRLDIKNDPTYAQEVG
                     EMPEVKAPEEIFYELSKEQSHFYDRVIEEYFGEGEDGRFIGAIYTPYRYEKDELRKKR
                     RKEEEEEGENIGDLEREMQDNLKEFMRRLAVKRFESSFAAFRKTMENFVSVGNRVLEF
                     IKKTKGKFILDRRAMEKLLEAEDEKISERLDEYIEKMLVQGEKRYAHIYHINEDFEEP
                     RQFMGDIRKDIDLFTEICEEIDRLHLIENDPKLDALVRKIPEILDKREKRVPDRKIVF
                     FTEYTDTLHYLAERLTLRFGSEILVMPDNLSDRAIKTILENFDASQPNQRNEAKILLA
                     SDKMAEGFNLGRAGAIINYDIPWNPTRVIQRVGRINRIGKKLFNHLYIYNFFPTEKGG
                     DVIKLRETAQQKMFMIHNALGEDARIFEPDEEVTPATLFKKIQRNPEEMEEESFLTTA
                     RRTYEELKKAHPDVVTKLAEVAPRVKSAKQFEKDNLLVFIRKGYSFFVRGIFDEKKTE
                     ELAFPEALPLIACGPEEEPLTFSSAAWERYRYLVERMSDPASVAPRNDISIKAAANLN
                     RILDNLPPGAEEYRSFVAMLLEDIQEYKTLAKHTLQRISRLPHKKPKELTETLKELRV
                     MLGKDYLRTIKESVRKLESEVIIAIENRTQE"
     gene            6803..9181
                     /locus_tag="PKH10_00800"
     CDS             6803..9181
                     /locus_tag="PKH10_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26690.1"
                     /translation="MARELLQKVAERFDRKELTRFFRAANDNFVQQLSPLRGYDSELF
                     TDFSVIGDMELESHRKLVTLAAKVSGNLNEHSSRKAQFDTVRKILGDRYPSYWGAICV
                     FHDNAGSFRFSLIHRQYEDTRVTFNPFRRYTYFIHPDEPNRTFLERVGGADFSTSILI
                     KEAFSVQKVTDEFFDNYLDIFNDLQGRLFKVSKDKSWAHDYALQVLNRLMFLYFIQKK
                     RWLGGNPQFIRYFWDSYKRSKQKKDSFFKNWLSVLFFEAFNGQFKAGRADRSYLPEDL
                     RGMLATAPYLNGGLFAPNKLDGQYTADLPDEFFEKLFDNYKGGTPGFFERYNFTITEA
                     TPFDVDVAVDPEMIGKVYEQLVNITFEGISEEDRRGGGGIFYTERVEIDLMCRLSLVD
                     CLTNYLGSEYRSLLYDAVFAHDPEEKNRAAAAFSEHNLWPSFSQVLKDLTVCDPACGS
                     GSFLIGMLMVLDDLQARANMQLGEEETAYRRRRRIVGNQLYGVDVMEWAVHVAELRLW
                     LQLAIETDLKPEELTLQPLLPNLSFNVRCGDSLVQEIGGINLGLRRTSFDIPFEIRGR
                     ITALQGKKKRFYQAESGIKKGELEREEKNIFLALLESKCLALKNEIKILSNRKGKDEG
                     QEGFAGFGEVKSKSKKDMAKLEEDIERKEEELTELKDSIEAMSGVETLPFVWDIAFVE
                     IFKGSQKGFDIVVGNPPYVKHEKIAPPNEDPKRFGGENADKWKKRKKAYKEKLQKSVA
                     LAWPCFFEYNMKKGIFRHAAGKSDLYIYFNAYPRFTGAERGNDSKKRFLFCL"
     gene            complement(9152..10621)
                     /locus_tag="PKH10_00805"
     CDS             complement(9152..10621)
                     /locus_tag="PKH10_00805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006198692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNT26691.1"
                     /translation="MNKLYFGDNLHILRDHIADESVDLIYLDPPFNSKANYNVLFKTP
                     AGHESTAQIQAFEDTWHWNEQAESEFREISHQSNTDVAALMQSLRQFLGENDMMAYLT
                     MMANRLLELHRVLKPTGSIYLHCDPTASHYLKILMDAVFEGKNFRNEIIWSYKRYTAQ
                     SNRFQRLHDVILFYGRSEKGVFNEPRDEYGPNSGKADSHYKQDEDGRWFRWQKRKGQE
                     PYKIFLSEGKRLGDVWEISHINASAKERMGYDTQKPQLLLERIVEASSNEGDVVLDPF
                     CGCGTAIHAAQKHNRQWIGIDITHLAITLIEKRIKDAFPGIVYEVEGTPKDFEGARNL
                     ALRDKYQFQWWACSLVNAQPYKGKKKGADGGIDGLIYFEDDKTGIAKKIIVSVKGGDN
                     IGRTMIADLKNSVDREKAQIGIFITLVAPTRPMEQEAASAGYYESPIGKTYPKIQILT
                     IEELLAGAKRPEYFDLTAGGLNFKKTKKESSQAKQESLF"
     gene            complement(10697..11629)
                     /locus_tag="PKH10_00810"
     CDS             complement(10697..11629)
                     /locus_tag="PKH10_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HNT26692.1"
                     /translation="MQKKAMSLLEFQKMFGTERKCSRYLQAQKWPEGKYVCPRCGGTE
                     SYPINARHQYECKRCHHQTSLTAGTIMHKSKTPLVKWFWAIYLSSRDKGGISALGLSK
                     HISVTYKTAWLMLHKIREAMKSRDAEYLLSGIVEVDEAYFTGSKKNEDDKNVEPVETK
                     RGRGTDKAKVLISVGTNPETNRPSFAKMTVVSDVTGRTLIGGIEKTVTAGSTIRTDGY
                     AAYNALSEAGYKHKTTDDTMKWVHIVISNAKTFLRGTYHGACKNHLQSYLDEFCYRFN
                     RRFWEDQLFTRTLYACALAKPFPLNALVERGRKG"
     gene            11746..13140
                     /locus_tag="PKH10_00815"
     CDS             11746..13140
                     /locus_tag="PKH10_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26693.1"
                     /translation="MLNKKSSFCFITSNSWLDANYGSDLQEILLKHGHIKLIIDNEKK
                     RSFKADVNTIIALLSPLDDRRDLGLNNTARFVMFRVPFEEVIDAETFKTLESVTTDRQ
                     NTEKWRICVKSHCELFADGLKRQEEEEEEEGEGEEENELIAKLKTVHVKTNKYMANKW
                     GGKYLRAPEIFFTVLDKGKDKLVRLGDISNVKPGCYSGINDFFYLDKQSAKQLGIESR
                     FLKPIIRNTSDAISPFITLTDLDTVVFSCNLDKDELQSFKGANNYIDWGARQVTRARQ
                     KTEAGIPWPETETVKRRTPGWWTIPRDNLTTTRNFLLYVHNERFIAPFTNKPVTSDRC
                     FHRIFLKEPGQEASLAAILNSTVTWLMICVYGRWGLGQGAMKFETSDACKLLLLNPAT
                     LNAITQNNLATCLETLGKRAVQAVKDEVLLSDRHALDSVVFDVLRLTSGEREAVYEAV
                     VNLVRARLEKAKSV"
     gene            13169..13630
                     /locus_tag="PKH10_00820"
     CDS             13169..13630
                     /locus_tag="PKH10_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNT26694.1"
                     /translation="MKKNFNEHLAELPKLLMNLHDAPLISFPYSEAPTKGIYVFYKKG
                     KPQYVGRTNRMKDRLQEHGRNSSNHNSAQFAFLLARKQASGKGIEIRNIPRKELEKEP
                     EFKKLFIAAKDEVRHMEVRCVEIKDPVTQALFEIYAHLELATHEYNDFDNH"
BASE COUNT         3582 a         3466 c         3475 g         3171 t
ORIGIN      
        1 agattctttc cactcgatgt tctgatcttc tcttgtccgg atcattattt tctcgacacc
       61 tccccaaaga tcaagacaaa actaattctc cgaaaagata agtcaagaaa gcaggcggcc
      121 attacttccg gtcgatttgt tcgaccgcgg aaaaggatat cacccccgcc cttttccgac
      181 gcaacgggga taatgatgtc tatcacaagt tatttcaagg tattgacaag acacggggaa
      241 actttttgaa taaaggacgg tctatctgga tactctaggg ttgtgagtcc cggagcgtga
      301 tctcttcgca cgctggtctc ttccgaaatt gcagggccct ttcccggggc cctctttttt
      361 ttgcctttcg ggcctctcgc cggtactatc gtcccggacc gattgacgga gccgcccatg
      421 cccaccgatc tcgatatcgc ctcgcaggtg tcgctcaggc ccatcgccga catcgccgcg
      481 aaactgggac tgcgccgcga acatctggaa ccctacggcg accacatcgc caaggtgaga
      541 ccgaccgccc gcgcgctggg cgagccgcag gccaaacagg tgctcgtcac cgcgatgacc
      601 cccaccccgc tcggcgaagg gaagaccacc gtcgccatcg gcctgtcgga ggccctgaac
      661 cgcatcggga aacggtccat cgtgacgctc cgcgagccgt cgctcgggcc ggtcttcggc
      721 gtcaaggggg gcgcggcggg cggcggctgg tcgcaggtcc tgccgatgac cgacatcaac
      781 ctccacttca ccggcgacat ccacgccgtg acgacggccc acaacctgct tgcggcgctc
      841 atcgacaacc agtattcccg cgtcacccag cagtgtctcc tgccgcgcga gatcgtgtgg
      901 caccgcgtga tggacatgaa cgaccggtcg ctccgcaaca tcgtcatcgg gctcggcaag
      961 gaatcggggc aggtgcgcga gagcaaattc gagataaccg cctcctccga ggtgatggcg
     1021 atactcgccc tcgcctacga cctcgacgac ctcaaaaaac ggctcggcga catcatcgtc
     1081 gcggtcaaac ccaaaggcga gccgctccac gcccacgacc tgaacgccca cggcgcgatg
     1141 gcgatactgc tcgccaacgc gataaagccc aacctcgtcc agaccatcga gggcaacccc
     1201 gccttcatcc acgccggccc cttcgccaac atcgcccacg gcaccaattc gatcatcgcg
     1261 accgacctcg cgcgccgtct ttcggacatc gtcgtgaccg aggcgggctt cgcctccgat
     1321 ctgggcggcg agaagttctt caacctcgtc tcgcggcaaa aaggcatgac cccgcccgac
     1381 gcggtggtgg tcgtcgcgac cgtccgcgcc ctcaaatatc acggcggggt ggtgcgcaag
     1441 gacctcggcc gacccgaccc cggcgcggtg gaacggggct tcccgaacct cgaaaagcac
     1501 atcggcaaca tgcgctcgtt cgaccggccg gtcctcgtgg ccctgaaccg gttccccggc
     1561 gacagcgacg acgagatagc ggcggcgcga cggctctgcg aacggcacgg cgcggtctgc
     1621 ttccccgtcg aggtgtgggc caaagggggc gccggggcga ccgaactcgc ggcggcggtg
     1681 tggggcgcgg cgcacgatgc gcacggcccg gtgagataca cctatgaact cgacgacccg
     1741 gcagcggtca agatagagaa ggtcgccaag gcgatgtacg gcgccgaggg cgtccatatc
     1801 ccccgctcgg tcctcgccaa gatagaggag cgcgaaagtt ggggctatcg cggactcccc
     1861 gtctgcatgg caaagacgca gtcgtcgctc tccgacaccc ccgatctccg ggggcggccg
     1921 accggcttca ccgtcgagat acgcgatgtg aaggtgagcg ccggggccgg cttcatggtg
     1981 gcctacacga gcgacatcat gaccatgccg gggcttcccg aaacgcccgc ctccgaccgg
     2041 atggatatcg acagcgacgg cgctatcagc ggcctgttct gaaaatttcg tgcggggaca
     2101 cccggcgtcc cggccggcat aataccctca tccctgcctt ctccctcaac aggtagaagg
     2161 atacacggac ttcctcgccc tgtggaggga gaggagtgag gtgagggtgt tcggtcggtg
     2221 cggtggtgga aaaaattgca tcgagcgggg cgcgggggta gagtgttgcg cggagaagtt
     2281 cagaagggag ttcgggaagt gtcggaaagg gtgaagatgg cggggcaacc gatcatccag
     2341 ccgcaaggcg agatagtcat ctatcagaat cccgacggca acatccggct cgatgtccgt
     2401 ctcgaagaag agaccgtctg gctgacacag gcacacatgg cgatcctctt cgataaggcg
     2461 aagtcgacga tcaacgaaca catccagaat atctttgaag agggcgaatt ggtcgaaggg
     2521 caggtcatga ggaaattcgg aaatcccgaa ttttccacca aaccgaccaa ctactacaat
     2581 ctcgatgtca tcatatcggt cggttaccgc gtcaaatcgc cgcaggggac ccagttccgc
     2641 atctgggcga ccaagcggct gaaagagtac atcgtcaaag ggttcgcgct caacgacgag
     2701 cgattcaaga ccggcaactc gatgaactac ttcaacgaac tgcaggaccg tatccggcag
     2761 atacgccttt ccgagcggtt cttctaccag aagataaagg acatctacgc gaccagcatc
     2821 gactacgacc cgcaggacga gcggaccatc gagttcttca aggtggtgca gaacaaactg
     2881 ctctgggcca tcaatcagca gaccgccgcc gaattgatct accgccgggt cgatgcgacc
     2941 ctgcctctca tgggaatgcg ctccttcgac aaggcgggga tcgcgaagat aaagaagagc
     3001 gacatcgcca tcgcgaagaa ctacctgacc gaggaagaga tgaaactgct cgggctgttg
     3061 gtcgaacaat acctcgcgtt cgccgagacg atggcgcaac agcacaagcc gatgtacatg
     3121 agcgattgga tagcgagact ggatgagatc ctgcggatga acggcagaga actcctgacc
     3181 cacgccggga agataagcca tgaaacggcg attcagaagt ccgagaccga atacgagaaa
     3241 taccgcaaga acgaacagcg tgccgaaaaa gaggcgagtc tcgccgagat agaaacggac
     3301 atcaaacagt tgaagcagat aacgaagaaa gggaaatgaa agtgaggcgc ggggcgggga
     3361 agttcgcgaa gagattcggg aagcggggga aaaagtgaaa taacacggcc cgataaagag
     3421 ggaaagcatg ttcatcacaa atgaaaaaac aaacacctta gaaaaacgac tgaatgagtt
     3481 ggtccccatc agccgggaac ttaaatttct tgtaggattc ttctatttct ctggtattcg
     3541 cgggatatat tcttcgttga aaaacaaccc tgaagctacg ctcaaagttc tggtcggtct
     3601 tgatacggat gcgactatcc ccaaaatggt ggtcgaggtt cccaagaacg atgagacaat
     3661 gagcaatgaa aggagccgtt cctgctatct ggagtcgatt aagacaggaa tgcgtcagaa
     3721 agattttgat tctgaggagt gttatgaaca gatatcgttt tttctcgacc tcctaaaaga
     3781 agggcggctc cagataaaga aaacgctcga ccccaaccat gccaagttgt atatcttcaa
     3841 tttcaaagag agttattccc agctccgttc acagttattt atctcaggca gcagcaatct
     3901 cacacgtgcc ggtcaggtcg ggcaaggcga actcaatgtg gagatcagcg attttggcct
     3961 taccaaggcg gcggaagacc attttaacaa gttgtgggag acagcgatca agataacgga
     4021 gcatgaaccg acgcgagatc ggcttatcaa aatcattgct gagcaaacct taatttcgcg
     4081 tctttctcct tttgaggcat accttctggt gctaaaaact tatcttgagg tcttgacggc
     4141 caccaatcac acggttgcct tttcgcaatc actcgaagaa aaaggttatc gggagtttcg
     4201 ctatcaaaac gatgcagtgg ctcaggcact tgacattatt gaagagaacg gtggagtggt
     4261 cattgccgat gtcacagggc ttggaaagag cgttattgca agtcgtatcg cccatgagat
     4321 gcgcgaaaga ggcatcatca tctgcccgcc ggccctcatt ggtgacgagg cgcggacggt
     4381 cggttggaaa aaatactgca aagacttcga tttactcaat tgggacattt tttcgcgagg
     4441 caacttggaa ggagcctttg agtgtgttgc gaatgacccc agcattaaag tcgtcatcgt
     4501 tgacgaggcg caccactttc ggaacgaggg tatttactcc tacgaacaac tcaaggcaat
     4561 ctgccggggt cgtaaagtca ttctccttac cgcgaccccg ttcaacaaca ccccggccga
     4621 cctgttcgcg ctgctgaaac tgttcgtcgt gccggggaaa tcggtgctta cccttgacga
     4681 agaccttgaa ggtcgatttc gaagttatcg taacctgtat gcaaaacttt cttatatcct
     4741 caagtattac aacagcaaaa aacccgaaaa aaggattacg gcagagaatt actatcaacg
     4801 actctttgga tctttgcctg tggatgttcg tcttgtaagg gaagaaacaa ggaaactttc
     4861 tcaacgaatc aaagatacaa tacgaccggc cgttattcgt cggaaccgcc ttgacatcaa
     4921 gaatgatccc acctatgcgc aagaggttgg cgagatgccg gaggttaagg cgcctgaaga
     4981 aatattctac gaactctcaa aagaacaatc acatttttat gaccgggtaa ttgaagagta
     5041 ttttggtgaa ggtgaagacg gtcgatttat cggtgccatt tacacgccgt accggtatga
     5101 aaaggatgag ttgcgcaaga agcgtcgcaa ggaggaagag gaagaagggg aaaatatcgg
     5161 agaccttgaa cgtgaaatgc aggacaactt gaaagagttc atgcgacgcc tcgcggtcaa
     5221 acgtttcgag agttcgttcg ccgccttccg caagacgatg gaaaactttg tctctgtcgg
     5281 aaatagggtc ttggaattca taaagaaaac taaaggcaag ttcatcctcg atcgacgcgc
     5341 tatggaaaaa ctcctcgagg cggaagacga aaagatctcg gaacgactcg atgaatatat
     5401 cgagaagatg ctggtacaag gcgaaaagag atatgcccac atctatcaca tcaatgaaga
     5461 ctttgaagag ccgcgacagt ttatgggtga catccggaag gatatcgatc tgttcaccga
     5521 gatatgtgaa gaaatagatc ggttgcatct catcgagaac gacccgaaac tcgatgcact
     5581 ggttcggaag attccggaaa ttctcgacaa acgtgagaag agggtgccgg accgcaaaat
     5641 cgtctttttc accgaataca ccgacaccct tcattacctc gccgagcggc tcaccctgcg
     5701 ctttggtagc gagatccttg tcatgcccga caacctgagc gaccgggcga tcaagaccat
     5761 ccttgagaac ttcgatgcct cgcagcccaa ccagcggaac gaggcaaaga ttcttttggc
     5821 gagcgacaag atggccgaag gtttcaacct tggccgcgcg ggagccatca tcaactacga
     5881 tattccatgg aatccgaccc gggtcattca gcgggtcggg cgcatcaacc gcatcgggaa
     5941 gaagttgttc aaccacctct acatctataa cttctttcct accgagaagg gcggcgatgt
     6001 cattaaactg cgcgagaccg ctcagcagaa gatgttcatg atccacaacg ctctcggcga
     6061 ggatgcgcgg atatttgagc ccgacgagga ggtaacaccg gcgacgctct tcaagaagat
     6121 ccagagaaac cccgaagaaa tggaagaaga gagttttctc acgacggcac ggcgaaccta
     6181 tgaggaactg aaaaaggcgc accccgatgt ggtcacaaaa ctggcggagg tggcgccacg
     6241 ggtaaagagc gccaagcagt ttgaaaaaga caatctgctg gtgttcatcc gcaaggggta
     6301 ttctttcttt gtacgcggaa tattcgatga gaaaaagaca gaagaacttg ccttccccga
     6361 agcgctcccc ctcatcgcgt gtggccctga agaggaaccg cttacattct catcggcggc
     6421 atgggagcgc tatcgctacc tcgtcgagcg catgagcgat cccgcctcgg tagccccccg
     6481 caatgatatt agcataaaag cggcggcaaa cctcaaccgg atcctcgata atcttccgcc
     6541 gggagcagaa gaataccgca gtttcgtcgc aatgctcctc gaagatatac aggaatacaa
     6601 gacccttgct aagcacacct tgcagcgcat atcccgactc ccgcacaaga aaccgaaaga
     6661 attgacagag accctaaagg aactccgagt catgttgggt aaggattatc tgcggactat
     6721 aaaagagagc gtccggaaat tagaaagcga agtcatcatt gcaatcgaga ataggacgca
     6781 agaatagagg gaggtcctga tcatggcgcg cgaactactt caaaaagtgg ccgagagatt
     6841 cgaccgcaag gaacttaccc gcttcttccg ggcggcgaac gacaacttcg ttcaacagct
     6901 ttcacctctt cgcgggtatg actctgaact cttcaccgat ttttcggtga tcggcgacat
     6961 ggaactcgaa tcgcaccgca aattggtgac tcttgccgca aaagtttcag ggaacctcaa
     7021 cgaacactca tcgcgcaagg cacagttcga tacggtacgc aagatactcg gtgatcgtta
     7081 cccttcctat tggggcgcca tctgtgtctt ccacgacaat gcagggtctt ttcgcttcag
     7141 ccttattcac cgtcaatacg aagacacaag agtaaccttc aatcccttcc ggcgttatac
     7201 atacttcatt caccccgacg aaccaaatcg gacattcctt gaaagagtag gcggggcgga
     7261 tttttccact tcgattctga taaaagaagc attctcggtc caaaaggtca cggatgagtt
     7321 tttcgataat tatcttgata tctttaacga tcttcagggg cgtcttttca aggtatcgaa
     7381 agacaaaagc tgggcgcatg actatgcgct tcaagttctc aaccgcctta tgttcctcta
     7441 ttttatccag aagaagcgat ggttgggggg caatcctcag ttcattcgtt atttttggga
     7501 ctcatataaa aggtcaaaac agaaaaagga ttctttcttc aagaattggc tatcagttct
     7561 gttctttgag gcatttaacg gccaattcaa ggcgggacgc gccgaccgca gttatttgcc
     7621 ggaggatcta cgcggcatgc ttgccacggc accatacctc aacggcggcc tgtttgcccc
     7681 caataaactc gatgggcaat atacggccga tcttccggat gagttttttg agaagctttt
     7741 tgataattac aagggaggta cgcccggatt ctttgaacga tacaacttca cgatcaccga
     7801 agcaaccccc ttcgatgtgg atgtggccgt tgaccccgag atgataggaa aggtgtatga
     7861 gcaactggtg aacatcacat tcgaaggaat ttccgaagag gatcgacgtg gtggcggtgg
     7921 tatattttac accgaacggg tcgagataga ccttatgtgt cggctcagct tggtggattg
     7981 cctcacaaac tatctaggct cggagtatcg gtcgctgttg tatgatgcag tctttgctca
     8041 tgacccagag gaaaagaatc gggccgccgc cgctttttcg gaacacaact tatggcctag
     8101 cttcagtcaa gttcttaaag atttgaccgt ctgtgatcct gcctgcggga gcggctcatt
     8161 ccttatcggg atgcttatgg tgctcgatga cctccaagcg cgggccaaca tgcaacttgg
     8221 ggaggaagaa actgcttatc gtcgtcgtcg tcgaattgtc ggcaatcaac tatatggcgt
     8281 tgatgtgatg gaatgggcgg tccatgtcgc cgaactgcgt ttatggttgc aattggcgat
     8341 agaaaccgat ctcaagcccg aggagctgac tttgcaaccg ctacttccta atctctcctt
     8401 caatgttcgc tgtggcgaca gccttgtgca ggagattggc ggcattaacc tcggacttcg
     8461 gcgtacttct ttcgatatac cgtttgagat acgagggcgc ataaccgcgc tccaaggcaa
     8521 aaagaagcgg ttctatcagg cagagtcagg catcaagaag ggcgaacttg agagggaaga
     8581 gaaaaacatc ttccttgctc tccttgagag taagtgtttg gcattgaaga acgagataaa
     8641 aatactttcc aacaggaagg gaaaagacga aggacaggag ggttttgctg gattcggcga
     8701 ggtgaagagc aagtcaaaaa aagacatggc gaagttagaa gaggatattg agcggaaaga
     8761 ggaagaactt accgagttaa aagactctat cgaggcgatg tctggagtgg aaaccctgcc
     8821 ttttgtctgg gatatcgcat ttgtggaaat attcaaagga tcccaaaaag gctttgatat
     8881 tgttgttggt aatcccccct atgtgaagca tgaaaaaatc gctccaccga acgaagaccc
     8941 caaacgattt ggcggcgaga atgccgacaa atggaaaaag cgaaaaaaag catataagga
     9001 gaaactccag aagtcagttg ctcttgcctg gccttgtttc tttgaatata atatgaaaaa
     9061 aggaatattt cgacatgccg ccggcaaaag cgacctttat atttacttta atgcctatcc
     9121 ccgctttaca ggggcggagc gggggaatga ttcaaaaaag agattcttgt tttgcttgtg
     9181 atgattcttt tttcgtcttt ttgaagttta atccgcccgc cgtcaagtca aaatactccg
     9241 gcctctttgc gcctgcaagc aactcctcta tggtgagtat ttgaatcttg ggatatgtct
     9301 tgccgatggg cgactcataa tatcccgccg aagccgcctc ctgctccatc ggccgagttg
     9361 gggcgacaag cgttatgaag attcctattt gcgccttctc ccgatctacg gagttcttta
     9421 ggtctgctat catggtgcgg ccaatattat caccgccctt caccgatacg attatcttct
     9481 tggcaatccc cgttttgtca tcttcaaaat agataagccc gtctatgccg ccatccgctc
     9541 ctttcttctt gcccttatac ggctgtgcgt tgacaagaga acatgcccac cattggaatt
     9601 gatacttgtc ccgcaaggcg agattccgcg ctccttcaaa atccttgggc gttccctcta
     9661 cctcataaac gatgccggga aaagcatctt ttatccgttt ctcaatgagc gtgattgcga
     9721 gatgggtaat gtctatgccg atccattgcc tgttgtgctt ctgggcggcg tgaatcgcgg
     9781 taccgcatcc gcagaatggg tcaagaacaa catccccctc attgctgctg gcctcgacga
     9841 tgcgctctaa aagcaactgc ggcttttgcg tatcgtatcc cattcgctct tttgccgatg
     9901 catttatgtg agatatttcc caaacatcac cgagccgttt tccctctgaa agaaagattt
     9961 tgtatggctc ttgtcccttc cgcttttgcc atcggaacca tcttccatct tcgtcttgtt
    10021 tataatgcga atctgccttg ccggaatttg gcccgtattc atctctgggt tcattaaaaa
    10081 cgcctttctc gctacggcca taaaaaagaa tgacatcgtg aagacgctgg aaacgattgc
    10141 tttgagcagt atatctctta taactccaaa ttatttcatt cctaaaattt tttccttcaa
    10201 acaccgcatc catgagtatt tttagataat ggcttgcggt gggatcgcaa tgaagataga
    10261 tacttcccgt tggcttcaac acccgatgca attcaagaag ccggttcgcc atcatggtga
    10321 gatatgccat catgtcattc tcgcctagaa actggcgtag tgattgcatg agggcggcga
    10381 catctgtgtt gctctgatgt gaaatttctc taaattcgct ttcggcttgt tcgttccaat
    10441 gccaagtatc ttcaaaagcc tgtatctgtg ccgttgactc atgccctgcc ggggtcttga
    10501 acaggacatt gtagttcgcc ttgctattga aaggagggtc aagataaatg aggtctacgc
    10561 tttcatccgc gatatggtcg cgcagaatat gaaggttatc cccaaaataa agtttgttca
    10621 tgcgtccctc catgtgtgga agcctttctc atgtcggcat aatatcaaag cgatggggaa
    10681 gcgtcaattt ttctgtctaa ccctttcttc cccgctccac cagcgcattc agcggaaacg
    10741 gcttcgcaag cgcacaggca tatagcgtcc gcgtgaacaa ctgatcttcc cagaatcggc
    10801 ggttgaaacg gtaacagaac tcgtcaaggt aagattgaag gtgattcttg caggctccgt
    10861 gataggtgcc gcgcaggaat gtctttgcgt tcgatatcac gatatgaacc catttcatcg
    10921 tgtcatcggt cgtcttatgt ttgtatcccg cttcggagag agcattgtag gcggcatacc
    10981 catcggtgcg tatcgtgctt cccgccgtca ccgttttttc tatgccgccg ataagagtgc
    11041 gtccggttac atcgcttaca accgtcatct tcgcaaaaga ggggcggttc gtttcggggt
    11101 tcgtcccgac actgataaga accttcgcct tatcggtgcc gcgtccacgc ttcgtttcga
    11161 ccggctccac attcttgtcg tcttcattct tcttactgcc ggtgaaatag gcttcgtcaa
    11221 cttctacaat acccgacaac agatattcgg catcgcgact cttcatcgct tctcgtatct
    11281 tgtggagcat gagccatgcc gtcttgtatg tcacgctgat atgctttgaa agcccaagag
    11341 ccgatatgcc gcccttatcg cgggatgaca agtagatcgc ccaaaaccat ttgacaaggg
    11401 gcgtcttgct tttatgcatg atggttcccg ccgtcaagga tgtctgatga tgacaccgct
    11461 tgcattcgta ctgatggcgc gcattgatcg gatacgattc cgtgccgcca caccgggggc
    11521 aaacatactt tccttccggc catttctgcg cctgtagata ccggctacat ttccgctcgg
    11581 tcccgaacat cttttgaaac tcaagtaacg acatcgcttt cttttgcatg actcactcca
    11641 aatctcaatg accacaagtt tatagtatca caaagattgg ggaaagtcaa cttttttctg
    11701 taaagagggg ataggcatat ttacttttac cttcacagcc tctcgttgct gaacaagaaa
    11761 agctcctttt gctttattac ttcaaatagt tggctcgatg ccaattatgg ttctgatttg
    11821 caggaaattt tgctcaaaca cgggcacata aaactcatta tagacaatga aaagaaacgt
    11881 tcgtttaagg cagacgtcaa tacaataatt gctttgctct ctccgctcga tgacaggcga
    11941 gatcttggcc tcaacaacac ggcaaggttt gtaatgttca gagtaccatt tgaagaagtg
    12001 atagacgccg aaacctttaa aacactagaa tccgtcacta cggatcgaca gaacaccgaa
    12061 aagtggcgta tctgtgttaa gtcgcattgt gaattatttg ctgatggcct gaaacgccaa
    12121 gaggaagaag aagaggaaga gggagagggg gaagaagaaa atgaattgat tgccaaacta
    12181 aaaaccgtgc atgtcaaaac caacaaatac atggccaaca aatggggcgg caagtatctt
    12241 cgagcgccgg agattttctt taccgttctc gataaaggta aagataaact tgttcgtctg
    12301 ggcgacatct ctaacgtcaa accggggtgt tattcgggaa ttaacgactt tttttatctt
    12361 gacaagcaat ctgctaaaca gttaggaatt gagagtcggt tcctcaaacc aattattcgc
    12421 aatacgagtg atgcgatttc gccttttata acattaactg atctcgacac agttgttttt
    12481 tcctgcaatt tagacaaaga cgagttgcaa tcttttaagg gggccaataa ctacattgat
    12541 tggggggctc gtcaggtaac acgcgcccga caaaaaacag aagcaggcat tccttggccg
    12601 gaaactgaaa cggtaaaacg aagaacgcct ggctggtgga caattccaag agataacctt
    12661 acaacaacgc gcaatttctt gctgtatgtt cacaatgagc gttttatagc accttttaca
    12721 aataaaccag tcaccagtga caggtgtttt catagaattt ttctgaaaga gccaggacaa
    12781 gaggcttctc ttgctgccat tttaaattct actgttacgt ggctcatgat atgtgtttat
    12841 ggtcgctggg gacttggcca gggtgcaatg aaatttgaga catcagatgc gtgcaaactt
    12901 ttgctcctaa atcctgctac cctcaatgcg attacgcaaa acaacttagc aacatgtttg
    12961 gaaacactgg gcaaaagagc agttcaagcg gttaaggatg aagttctttt gtcagaccgt
    13021 catgcgttag atagtgttgt gtttgatgtg ttgagattga cctcgggtga gcgcgaagcg
    13081 gtatatgagg cagtggtcaa tcttgttcga gctcgactgg aaaaggcaaa gagtgtgtga
    13141 atagcaaaca attaatggta gggagaaaat gaaaaagaac ttcaatgaac atcttgcaga
    13201 actacctaag cttttaatga atctccatga cgcccccctc atatcttttc cttattccga
    13261 agctcccaca aaaggcattt atgtttttta caaaaaagga aagcctcagt atgtgggtcg
    13321 cacaaatagg atgaaagacc ggcttcaaga acatggccga aatagttcaa accacaactc
    13381 tgctcaattc gcgtttcttc ttgcccgaaa acaggcttca ggaaaaggca tagagatcag
    13441 aaacattcct cgcaaggaac tagaaaaaga accagagttc aaaaaactat ttattgctgc
    13501 caaggatgag gtccggcata tggaagtacg gtgtgtggag ataaaagacc ctgtcacaca
    13561 ggcattgttc gaaatatatg ctcaccttga actagcaacc catgaataca atgattttga
    13621 caaccactga agattataat ggaaacaccg tatatcctcc agaggttgtt ctccgacgag
    13681 aagtttatct ctct
//
LOCUS       DAOQUE010000013        13619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_5093_length_13619_cov_4.440357, whole genome shotgun sequence.
ACCESSION   DAOQUE010000013 DAOQUE010000000
VERSION     DAOQUE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13619
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5093_length_13619_cov_4.440357"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PKH10_00825"
     CDS             <1..1190
                     /locus_tag="PKH10_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26695.1"
                     /translation="IEERWPLSECTPKIYDQCAENYPHEAVTRAPCGEFISTDLDPKI
                     LQNSYDYILTPYCWDTSSMEFPLYSLNGGLLSFYSSTASPYDSGYFTYDLVSRKLSRI
                     GRGMAVSPFSNGEYFFARTTDYSTVWEESYEEVVFYYHFGNKTYGNAWKMPQKLSYVR
                     NIVASDQYIAMNLVPLTGNRNEPLRGYYTKIGEWDKWVELNFGTYPSYGIGRPEIQGS
                     YLVSYNYIDNLLRVFFCDLAKGESSCRIVSKAGETARKPIWGIENTFYYSVETENEGA
                     TVYQATVEVTGEQETTATSAPLFSSDEYMQVYQANEDFLLYLKFIKAGSPSDIYNLCY
                     YRFSDKKHFCLEDGINSDIEYQDPYFHDKWIVFRSKYDLIIRDMNGYCKEHSANCPFE
                     ELK"
     gene            1187..1963
                     /locus_tag="PKH10_00830"
     CDS             1187..1963
                     /locus_tag="PKH10_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26696.1"
                     /translation="MMLLALMLLTISEYDLLQKAAADGFLYRNVAVETVEKCPAKATC
                     ITAEGSTYLLKRLDTTAVTLEKSADGFALTIAPDKNFTNYRLFIREESTVRRIEVTQK
                     RVTVLDKGSVTALQLVGVGAQGPVVIWQKDVRATAIEPFTGDLLGSMNVLRKKAGLPP
                     LTIDKTLAPFAAETIARVKKGDLSHYSGSTGSIRHSGVRAKELGENLFSATTLERAWL
                     MMLASPSHLFNILSPSFRRCFVGTLPAESGLIPGIVIFAD"
     gene            2027..2770
                     /locus_tag="PKH10_00835"
     CDS             2027..2770
                     /locus_tag="PKH10_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HNT26697.1"
                     /translation="MKIFFLTPYHGEAPASDDVTRIAAALPKIDATVTTGTLDLRGVD
                     LDAMPLDKARSLFEKHHGRMAAADIIHSFSRAALLLRPLFPATLIVTILDTEAVASFL
                     PRPGEPGIEMLPVAECRDGATLLGHYRRILNNKKRHDTRPWGSWESLRLMDDHKVKHI
                     YVAPGEKLSLQLHHRRSEVWTVVAGSGTITVGDKALPAGPGQVFIIGKEQIHRAEGGP
                     DGLHFIEVQTGDYLGEDDIVRLEDRYGRS"
     gene            2885..4948
                     /locus_tag="PKH10_00840"
     CDS             2885..4948
                     /locus_tag="PKH10_00840"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNT26698.1"
                     /translation="MASYTSDKLRNVCVAGHGTTGKTTLVESIMFQAGAINRQGTVEA
                     GTTVSDFTEEEMNRGISISAAVCSVEYRKELVCLVDVPGYFDFSGELAASMPFCEAAL
                     LTVKGTAGVDVGTEKAFNLARRYGRSVAFFINEIDKENLAADEVIAGIESALGIRVAP
                     LSYPGAVGPAADTVVDVLKGKAFKYAEGKRAGEGALSDDVAAMIAEFKTKIMEAVAES
                     DEALMEKYFAEGTLSDDEVTIGLGKAFAKGTICPLFFGAPNKGIGTDIVLDAIIDFFP
                     SPAVKPDITYPDTTVTLNPAEPPRAVVFKVNVIPQFGEVFLFKMLAGELAEATEFYNV
                     SSSASEKFGQLFAVRGKERTALNKVVAGQIAATVKLKNTRTGDQFVKDKNAQPLPLKL
                     IEWPEAIVRGAFVGESKEDTDKVATNLRRIQIEDASFRLTADPELKQLFVDGLGELHL
                     DVLAKRLRNQYKLSVRIEEPKIPYRETIRGNSETSYRHKKQTGGSGQFAEVHLRLEPN
                     PEGFEFVNAIVGGVISGRFIPAVEKGVREVMEDGVFAGYKFINCRVTLFFGKEHEVDS
                     NEMAFKIAASMAFKDAVMKAKPILLEPIYTVEVEVPDEYAGSIMGDISSRRGKPQGMD
                     SRGRNQVVRALVPLKEMHGYSTQLRSMTNGRGLYTISFSHYDPVPPDIQQRLMDEYQR
                     SRSQQ"
     gene            4945..6930
                     /locus_tag="PKH10_00845"
     CDS             4945..6930
                     /locus_tag="PKH10_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNT26699.1"
                     /translation="MRPTDRRSSLISPNTLLFISATLFVLLAGCKACKQDPDEQPISA
                     TDTVDPQAEIYRVERGDSPLIGDAAAPVTVINFSDFQCPFSKRAFELFERLLKEKGDR
                     VAYVYKHYPLATHREGRRAAKAAIAAARQGKFVEMYRKLFENNLAISEENITRWAQDI
                     GMDADAFARDYADPDTDRRLHEDVTTGNIFGVRGTPTIFINGRRISGANTDQIERLIA
                     EEMEKGESLKKNGVTDPYADIIKNGLSRYVPPKRPLPVVPKDIYAVAIPPHAPQRGAP
                     GAIITLIIFTDLECPFCARFVSTLSELEKEFEGKLRVISLNLPLTIHPLAVQAAKAAI
                     AAGRQNKFWEMHDTLFAEQPEWKKGANFDQYLDGVAQRLALDMVKFKKDMEDPRSMEI
                     IQRDIALADLIGVRGTPGIFINGRYASGAFPIETFQVTIREEIERARPLIDAGTSGDA
                     LYHELIRNGKEAVFENSPAAKDAGRVHTIKLSGQEPSVGKKSAPVTIVEFCDLQCPFC
                     AKMSVSLDEIQKEYRDSLRIVHKQFPLPQQHPLSEEVARIALSVKKLYGDDRYRTLRG
                     KLFAKQSEWTGSVDGIGTIRKILVELGIDPDKVQKEKISPAIETILSEDRAEAAKNNV
                     RSTPVIFINGRKVNGSQPPERLKVLIEEALKEQQAKK"
     gene            complement(6973..8871)
                     /locus_tag="PKH10_00850"
     CDS             complement(6973..8871)
                     /locus_tag="PKH10_00850"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNT26700.1"
                     /translation="MDEKKVEGGKPAIDKEYLKKLAEKIKAEKAKREGGQSVETESLP
                     PLPPQMTAPKPPLPPLPPSSRPELADDDDDEKEGMAAEKTAIIDLAALSGQNADARIV
                     ITDGKDEGKALDLTRDEVICGRSLDNDFVISDISVSRKHFKIVRDTDGFKVEDMGSGN
                     GIRVNGKKETSTLLFDGDLVTAGARTIRFEVLNEALKEKYSRKMPSGADVEVIMAKSR
                     TPVVAWIAILLVLVVGGAGVYLFYTQHQQQQELRLKLAQEDLLDRIDQLIEERKFADA
                     ERSIDEFLMKYPGVKVREITKNKALVQKEKTYASRFDQGQKYLSENNTDEAYRAFRLI
                     PDDSIFYPEVVALVGKETVDSWVFSQIEELIKTNKTDDAVRKISALLVDDPANKKAAE
                     LMAQVKAQVGAQKVETIQTTVAKEKEEKKKEIAEREQRKKELISKGKIKAAPKPRPVV
                     VKKPEPKPEPESIVDTKPEYTMDDINSAIDTYAYKDFDGAISRLESMTGTKDKKVAKK
                     AKDLIDKIKKFKQAYDKKDAKKATIFDKQISGGKLKDDITELNKKESAPEKKRSDSVD
                     EFASGGSSSDGGGGSYDEGKAKTLYMEAVSLKESDPEAAKEKFKEVMKLVPNDNKYYK
                     KAKKALKE"
     gene            9085..11043
                     /locus_tag="PKH10_00855"
     CDS             9085..11043
                     /locus_tag="PKH10_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26701.1"
                     /translation="MKGTALLFLIALSVFFSSCSDSTPQQPGDDEDAIGTTDETSESE
                     KGSALPDTADQETVDGEQDDALIADDDDEKDIDIPLNDDDDPGDDSLVDSDSAHAGMG
                     PGTENGYDDGSSNADNVKVNENGELELDNGSDSVQTLLRYVWVPNSADGTISKIDSFT
                     QVEVARYHAGLSALSNPSRTSVDLAGNMFVGNRDDSTVTKIAGHPEYCVDRDLSGTIE
                     TSTGPTDVLPRGVDGRSTDECVVWTRLFDASDLNSPYACGGIRGVAATPETGANFEYT
                     GHLMLGCYLGNPFSYRLNGNTGDIMQIFSMTSTTDQTCRPYGYVLDKYGRNWVSCRYI
                     PEWQTPVTGGLAWFDINDVTEQIHYVPDIPAYGGAGGYYGIAIDKDDNIWVTSYTDGF
                     VHRYTPDDSPFADNGTWTSLQISTAHGFRGIAADDSGFIWTISTEEGTGIDSRIYMID
                     SSIYPDPSSIHGPYILGDDTTHPFQGTGVGIDFKGNVWGMSRCNAGQGYVTYLPVDRS
                     TPVPTVVEAGKKIIAVGNGPYVYSDMIGYNLKNFASKEGWFRHIFEICASSLSTDWQQ
                     IYWDAEVPANTRVIIRARTSNDILKLATEPWQIIVEVPSDLSPKVIPDTLPEGHYIEL
                     EVRLYSKEVGISPVIGQIAFDYECVDPS"
     gene            11069..12295
                     /locus_tag="PKH10_00860"
     CDS             11069..12295
                     /locus_tag="PKH10_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNT26702.1"
                     /translation="MRYSLVFLLMLVPSFAGADELCGLPPELLPPPDSGQKCYDPGPA
                     DETEKDPKQVANIEEKKKEEEEKEEEKDKDSGIVAFPIAFYTSDTSFGFGGTAILFKK
                     SYETEEEKRSDSFTTVIFYTLNNQILNANAGTLYMDNGNFKANAVFVVSKYPDEFYGI
                     GPDSGWDDGEKYTPLTIRGEISFSARLWEKIYGGLTVLQGYHELMKYDRNGVLADYLR
                     DNRTDGFFSGLGLLFTRDSRDNSLYPRTGAVTTFSVLSFNRLLLSDYTFSTLLFDHRQ
                     YHSLPLDSVIAWQLHFNSVLTGKPPLPYLPALGSQNLMRGYPQNRYRDRNFMAAQVEW
                     RVPIWWRFGAVVFAGLGTVKGSIDDYLFTDPKFAAGAGLRITLSKKNNINFRFDFGMS
                     AERPYLKLYFNLLEAF"
     gene            complement(12349..12915)
                     /locus_tag="PKH10_00865"
     CDS             complement(12349..12915)
                     /locus_tag="PKH10_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNT26703.1"
                     /translation="MKKMLVLMAILGVALFIASCGSSEKAPANAMQNELDGAPEWVLA
                     ESAGGSGLICGVGSATGTRNVSLARTAAQGRGRTAIARALQVKVKSMLKDYQSTTTGG
                     EAFGQAANDEQHIEDVSKQITDTTLSGTEQKNTWISKTGTLYVQMCMDVEKFKGAINQ
                     MQQLDEKVRAAVVERANKAFNELDEATK"
     gene            complement(13086..>13619)
                     /locus_tag="PKH10_00870"
     CDS             complement(13086..>13619)
                     /locus_tag="PKH10_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26704.1"
                     /translation="LSYALYYKREKTGSLNDKDRLALYIKGRWNFTTDTDLFGWFYHE
                     YAVAPMRYVSRSIALQVRQWLFDRKLALSTGGVLLIEKHPGKKWYLPVWPFFEIQGHP
                     IVGLELSARLHLKFDEMMIRPPEWEYGLFQTRLEVGGGYLIGGYVRPAVYFFMNFAEG
                     AASPDSLGLKLSATAFF"
BASE COUNT         2971 a         4177 c         3640 g         2831 t
ORIGIN      
        1 gaatagagga acgatggcct ctttccgaat gtacgccgaa gatatatgac cagtgtgcgg
       61 agaactatcc gcacgaagcg gtgacgcgcg ctccctgtgg ggaatttatc agcacggatc
      121 tcgaccccaa aatactccag aactcctatg actatattct tacaccatat tgttgggata
      181 cctcttccat ggaatttcct ctttattccc tgaatggggg gctactttct ttttattcca
      241 gcaccgcttc tccgtatgac tcaggttatt tcacatacga ccttgtctct agaaaactct
      301 ctagaattgg tcgaggaatg gcggtttctc ctttttctaa cggagagtac ttctttgcca
      361 gaacaacgga ctactctacg gtgtgggaag aaagttatga agaagtagtt ttctactatc
      421 actttggaaa taagacttac gggaacgcat ggaagatgcc tcagaaactc tcgtatgtgc
      481 gaaacattgt tgccagcgat cagtatatag ccatgaatct cgtgcctctt acgggcaatc
      541 gcaatgagcc cctgcgcgga tactatacca agatcggcga gtgggacaaa tgggtcgaat
      601 tgaatttcgg gacctacccc tcctatggta tcgggcgtcc ggagatacag ggaagttatt
      661 tggtttccta taactacatc gacaatctct tgcgcgtatt tttctgcgat ctcgccaaag
      721 gcgaatcaag ttgcaggatt gtgagcaaag cgggagagac cgcccgcaaa ccgatatggg
      781 gtatcgagaa cactttttac tactcggttg aaacggaaaa cgagggagcg actgtttatc
      841 aggcaaccgt ggaggttact ggagagcaag aaacaacggc gacatcggct cctcttttct
      901 catcagacga atatatgcag gtataccaag caaacgagga ttttcttctc taccttaaat
      961 tcataaaagc aggcagtcct tccgacatat acaatctttg ttattatcgt ttttcggaca
     1021 aaaagcattt ctgtctggaa gatggcatta acagcgatat tgaatatcaa gacccgtatt
     1081 ttcacgacaa atggatcgtg tttcggtcaa aatacgactt aataatacga gacatgaacg
     1141 gctactgcaa agaacactcg gcgaactgtc ctttcgagga actgaaatga tgttgcttgc
     1201 actgatgcta ctaaccatct ccgaatacga ccttctgcaa aaggccgctg ccgatggctt
     1261 cctctatcgc aatgtcgccg ttgaaacggt cgagaaatgc cccgcgaaag cgacctgcat
     1321 caccgccgag gggtcgacct acctgctcaa acggctcgac accaccgccg tcacgctcga
     1381 aaagagcgct gacggcttcg cgctgaccat cgcacccgac aagaacttca cgaactaccg
     1441 cctgttcatc cgcgaagaat cgacggtgcg ccgcatagag gtgactcaga aacgggtgac
     1501 cgtactcgac aagggatcgg tgaccgccct gcaactggtc ggcgtcggcg cgcagggacc
     1561 ggtcgtcatc tggcagaagg atgtccgcgc cacagcgatc gaacccttca ccggcgacct
     1621 cctcggctcg atgaacgtac tccgcaaaaa ggcgggcctg ccgccgctca ccatcgataa
     1681 aacactcgcg ccgttcgccg ccgagaccat cgctcgtgtc aaaaaaggag atctttcgca
     1741 ctactcgggc agcaccggga gcatccgtca ctcgggtgtc cgcgccaagg aactgggcga
     1801 gaacctcttc tcggccacga cactcgaacg ggcgtggctc atgatgctcg cctcgccgtc
     1861 gcaccttttc aacatccttt ccccctcgtt ccgccgctgt ttcgtcggga cccttcccgc
     1921 cgagagcggg ctcatccccg gcatcgttat cttcgccgac tgaccgccat tgacgggacc
     1981 ttgtttttag cgtaggatgc gccatgacga ccgaacggag gccgcaatga agatattttt
     2041 cctgacaccg taccatggag aagcaccggc cagcgacgat gtcacgcgga tcgccgccgc
     2101 cctgccgaag atcgatgcca ccgtgacgac cggaacactc gacctgcggg gggtcgacct
     2161 cgacgcgatg ccgctcgaca aggcccggtc gcttttcgag aaacatcacg ggcggatggc
     2221 cgccgccgac atcatccact cgttcagccg cgccgctctc ctgttacggc cgcttttccc
     2281 cgcgacgctc atcgtgacga tactcgacac cgaagcggtc gcctccttcc ttccccgccc
     2341 cggcgaaccg ggcatcgaga tgcttccggt ggccgaatgc cgcgacgggg cgacgctcct
     2401 cggtcattat cgccgcatcc ttaacaacaa gaaacgacac gacacgcggc cgtggggctc
     2461 atgggagtcg ctccggctca tggatgacca caaggtgaag cacatctatg tcgctcccgg
     2521 cgagaaactc tcgctccaac tgcaccaccg ccggagtgag gtctggaccg tcgtcgccgg
     2581 ttccggcacc atcaccgtcg gcgacaaggc actcccggcc ggtcccggac aggtgttcat
     2641 aatcggcaag gaacagatcc accgcgccga gggtggtccc gacgggcttc atttcatcga
     2701 ggtgcagacc ggcgactatc tcggcgagga cgacatcgtc cgactcgagg accgctacgg
     2761 acgctcctga gccgctccgc gcgcgcgaaa ctaactgaaa aaacttgacg aaacggcact
     2821 ttatcattaa agccctctat gcttgcgact ttgatttgaa aagacacgac catcggagga
     2881 acgaatggct tcttacacga gcgacaagtt acgaaatgtg tgcgtcgccg gacacggcac
     2941 caccggaaaa acgacccttg tggaatcgat catgttccag gccggcgcca taaaccggca
     3001 gggaacggtc gaggcgggca ctacggtgtc cgatttcacc gaagaggaaa tgaaccgcgg
     3061 catctcgata tcggcggccg tctgctccgt cgaataccgg aaggaactcg tctgcctcgt
     3121 ggatgtgccg ggttacttcg atttctcggg cgaactcgcg gcatccatgc cgttctgcga
     3181 agcggccctg ctgaccgtca aagggaccgc cggtgtcgat gtgggcaccg agaaggcctt
     3241 caacctcgct cgccgttacg gtcgatcggt cgccttcttc atcaacgaga tagacaagga
     3301 gaacctcgcg gccgacgagg tgatcgccgg catcgagagc gcgctcggca tccgggtggc
     3361 gccgctgagt taccccggcg cggtcggccc cgccgccgac acggtcgtcg atgtgcttaa
     3421 aggcaaggcc ttcaaatacg ccgagggcaa acgcgcgggc gaaggcgctc tttcggacga
     3481 tgtcgcggcg atgatagccg agttcaagac caagatcatg gaggcggtcg ccgaaagcga
     3541 cgaggcgctc atggagaaat atttcgcgga gggcacgctt tccgacgacg aggtcaccat
     3601 cgggctcggt aaggccttcg cgaagggaac gatatgcccc ctcttcttcg gcgccccgaa
     3661 caaaggcatc ggcaccgata tcgtgctcga cgcgatcatc gacttcttcc cctcccccgc
     3721 cgtcaaaccc gacatcacct accccgacac gaccgtcacg ctgaacccgg ccgaaccgcc
     3781 gcgcgccgtg gtgttcaagg tcaatgtcat cccgcagttc ggcgaggtgt tccttttcaa
     3841 gatgctcgcc ggcgaactgg cggaagcgac cgagttctat aatgtgtcgt cctctgcttc
     3901 agagaagttc ggccaactct tcgccgtgcg cggcaaggag cgcaccgccc tcaacaaggt
     3961 ggtcgccggc cagatagccg cgacggtgaa actcaagaac acccggacgg gcgaccagtt
     4021 cgtgaaggac aagaatgccc agccactccc cctgaaactc atcgaatggc ccgaggcgat
     4081 agtgcgcggc gcctttgtcg gagagtcgaa agaggatacc gacaaggtgg cgaccaacct
     4141 ccgcaggata cagatagagg acgcgtcgtt ccgcctgacc gccgacccgg aactcaaaca
     4201 actgttcgtc gacgggctgg gcgaacttca ccttgatgtg ctcgccaaac gactccgcaa
     4261 ccagtacaaa ctcagtgtcc gtatcgagga gccgaagata ccctaccgcg aaacgatacg
     4321 cggcaacagc gagaccagtt accgccacaa gaagcagacc ggcggttcgg gtcagttcgc
     4381 cgaggtccat ctgcggctcg agccgaatcc ggaagggttc gaattcgtca acgccatcgt
     4441 cggcggcgtc atatcgggcc ggttcatccc cgcggtcgaa aagggcgtcc gcgaggtgat
     4501 ggaggacggc gtcttcgccg gctacaagtt catcaactgc cgggtcaccc ttttcttcgg
     4561 caaggaacat gaggtcgatt cgaacgagat ggcgttcaag atcgccgcct cgatggcctt
     4621 caaggacgcc gtcatgaagg cgaaacccat cctgctcgag ccgatctaca ccgtcgaggt
     4681 cgaggtgccc gacgaatatg ccgggtccat tatgggcgac atatcgagcc gccgcggcaa
     4741 gccgcagggg atggattctc gcggtcgcaa tcaggtggtg cgggcgctcg taccactcaa
     4801 agaaatgcat ggctattcga cccaactgcg ctcgatgacc aacgggcgcg gactctacac
     4861 catcagtttc tcccactacg atccggtgcc gcccgatatc cagcagcggc tcatggacga
     4921 ataccagaga tcgcggtccc aacaatgaga ccgaccgacc gcaggtcttc tctgatatcc
     4981 ccgaacaccc ttcttttcat ttccgcgacc ctcttcgttc tccttgccgg ctgcaaggcc
     5041 tgcaaacaag atccggacga gcagcctata tcggccaccg ataccgtcga tccgcaggcg
     5101 gagatatacc gcgtggagcg cggggattcc cccctcatcg gcgatgccgc cgccccggtc
     5161 accgtcatta atttctccga tttccaatgt cccttctcca agcgggcctt cgaactcttc
     5221 gaacgcctcc tgaaggaaaa aggcgaccgc gtcgcgtatg tctacaaaca ttacccgctg
     5281 gcgacccatc gcgaagggcg ccgcgccgcg aaggcggcca tcgcggcggc acggcagggg
     5341 aaattcgtcg agatgtaccg gaaactgttc gagaacaatc tggccatcag cgaggagaac
     5401 atcacccgtt gggcgcagga tatcggcatg gacgccgacg cgttcgccag ggactacgcc
     5461 gaccccgaca ccgaccgccg gctccatgag gatgtcacca ccggcaacat cttcggggtg
     5521 cgcgggaccc ccaccatctt catcaacggc agacgcatat cgggcgcgaa caccgatcag
     5581 atagaacgac tcatcgccga agaaatggaa aagggtgagt ccctgaaaaa gaacggcgtc
     5641 accgatccgt acgccgatat catcaagaac ggtctttccc gctatgtgcc gcccaaacgc
     5701 ccgctcccgg tcgtccccaa ggacatctat gcggtcgcca tcccgccgca tgcgccacag
     5761 cgtggggcac ctggcgccat catcaccctg atcatcttca ccgacctcga atgccccttc
     5821 tgcgcccgct tcgtctcgac gctcagcgaa ctggaaaagg aattcgaagg caaactgcgc
     5881 gtcatttccc tgaacctgcc tcttacgata cacccccttg ccgttcaggc cgccaaggcg
     5941 gccatcgccg cgggacggca gaacaaattc tgggagatgc acgacaccct gttcgccgaa
     6001 cagccggaat ggaaaaaagg cgccaacttc gaccagtatc tcgacggggt ggcgcaacgg
     6061 ctcgccctcg atatggtcaa gttcaagaaa gatatggaag acccgcgatc catggagatc
     6121 atccagcgcg acatcgcgct cgccgacctg atcggcgttc gcggcacccc cggcatcttc
     6181 atcaacgggc gctatgcgag cggcgcgttc cccatcgaga catttcaagt gaccatccgc
     6241 gaagagatcg aacgggcgcg acctcttatc gatgccggca caagcggtga tgcgctctac
     6301 catgaattga tccgcaacgg caaagaggcg gtattcgaga acagccccgc cgcaaaggac
     6361 gccggtcggg ttcacacgat aaaactctcg gggcaggaac cgtccgtcgg caagaaaagt
     6421 gcgcccgtca ctatcgtcga attctgcgat cttcagtgcc cgttctgtgc caaaatgagc
     6481 gtttcgctcg acgagataca gaaagagtac cgcgactccc tgcggatagt gcacaagcag
     6541 ttccctcttc cccagcagca cccgctatcc gaggaagtgg cacggatcgc cctttcggtc
     6601 aagaaactgt acggtgacga tagatatcgt accctgcgcg ggaaactctt cgcgaaacaa
     6661 tcagagtgga cgggatcggt cgacggcatc ggaacgatca ggaaaatcct tgtcgaactc
     6721 ggcatcgacc ccgacaaggt gcagaaagaa aagatctccc ccgccatcga gaccattctt
     6781 tccgaggacc gcgccgaggc ggccaagaac aatgtgcgtt ccacgccggt gatcttcatc
     6841 aacggcagaa aggtcaacgg gtcccagccc cccgaacggc tcaaggtgct gatagaagaa
     6901 gcactgaagg aacaacaggc aaagaaataa aggtagatac aaggaggaac gggaacctaa
     6961 tcgggaagcg ccctattcct tgagcgcctt cttcgccttc ttgtagtatt tgttgtcgtt
     7021 cggaacgagt ttcatgacct ctttgaattt ctccttagcc gcctcggggt cggactcctt
     7081 gagcgagacc gcctccatgt agagggtctt cgccttcccc tcgtcatacg agccgccacc
     7141 gccgtccgat gacgatcctc ccgaagcgaa ctcgtcgacg gaatccgatc tcttcttttc
     7201 gggcgcgctt tctttcttgt tcaattcggt gatatcgtcc ttgagtttgc cgcccgatat
     7261 ctgtttgtcg aatatggtgg cctttttcgc gtccttcttg tcgtaggcct gcttgaactt
     7321 ctttatctta tcaatgaggt ccttcgcctt cttggcgacc ttcttgtctt tggtaccggt
     7381 catcgattca agccgcgata tcgcaccgtc gaaatcctta taagcgtaag tgtctatcgc
     7441 gctgtttatg tcatccatcg tgtactcggg cttcgtgtcc acgatcgatt ccggctcggg
     7501 cttaggttcg ggttttttaa ccacgaccgg acgcggctta ggcgcggctt ttatcttgcc
     7561 cttgctgata agttccttct tgcgttgttc ccgctcggcg atctcctttt tcttttcctc
     7621 cttctccttg gcgacggtcg tttgtatcgt ctcgaccttt tgggcgccga cctgcgcctt
     7681 tacctgtgcc ataagttcgg ccgccttttt gttcgccgga tcatcgacaa gcagtgccga
     7741 tatcttgcgg accgcgtcat cggtcttatt cgtcttgatg agttcctcta tctgagagaa
     7801 gacccaggaa tcgacggtct ccttaccgac cagcgcgacc acttcgggat agaatatcga
     7861 gtcgtccggt atgaggcgga aggcgcgata cgcctcgtcc gtattgttct ccgaaaggta
     7921 tttctgaccc tgatcgaaac gggaagcgta ggtcttttcc ttttggacga gcgccttgtt
     7981 cttcgttatc tcacggacct tcacgccggg gtatttcatc aagaattcgt ctatggagcg
     8041 ctcggcgtcg gcgaacttcc gttcctctat gagttggtcg atgcggtcga gcagatcttc
     8101 ctgcgcgagt ttcaggcgca gttcctgctg ttgctgatgc tgggtgtaga acagatacac
     8161 gcccgccccg ccaaccacca gcacgagcag tattgcgatc cacgccacca ccggcgtacg
     8221 cgatttggcc atgatgacct cgacatcggc accggagggc atcttgcgcg aatatttctc
     8281 cttgagcgcc tcgttcagca cctcgaatcg aatggtacgg gcaccggccg tgacaaggtc
     8341 gccatcgaac aggagggtgc tcgtttcctt cttcccgttg acacggatac cgttgccgct
     8401 ccccatatcc tcgaccttga acccgtcggt gtcgcgcact attttgaaat gcttacggga
     8461 caccgatatg tcactgatga cgaaatcgtt atcgagactt cgcccgcaga taacctcatc
     8521 tcgcgtgaga tcgagcgcct taccttcgtc cttgccatcg gtgatgacga tgcgggcatc
     8581 ggcattctgg cccgaaaggg cggcaaggtc tatgatggcg gtcttttcgg ccgccattcc
     8641 ctctttttcg tcatcgtcat catcggcgag ttcgggccgg gacgaggggg ggagcggcgg
     8701 taacggcggc ttcggcgccg tcatctgggg aggcaacggg ggcagtgatt cggtctcgac
     8761 cgactgcccg ccttctcttt tcgctttttc cgcctttatc ttttcggcta atttcttcag
     8821 atactctttg tcgatcgccg gcttgccacc ttcaactttc ttttcatcca tcgcattctc
     8881 tcctaaatga acgcctcttt tttcttgcca gcaccatttt tcaaaaacaa tgccgaaagt
     8941 caactatttg gcgaataatt atacaatcgc ggcccaaccg acgattttga ctggaaaacc
     9001 gtttcttctt gcatttcgcc ccattatgga ctatatacca aactcatggt gatctcatgt
     9061 acgatcgagg gggggattcc aacaatgaaa ggcacggctc ttttgttcct tatcgctctt
     9121 tcagtctttt tttcatcctg ctcagacagc acgccgcaac aaccgggcga cgacgaagat
     9181 gccatcggta ccaccgacga gacatctgaa tctgaaaagg gatccgctct ccccgacacc
     9241 gccgaccagg agaccgttga cggtgaacag gacgatgcgc tcatcgccga tgatgatgac
     9301 gagaaggata ttgatatccc gttgaacgat gatgacgatc ccggcgacga ttcgcttgtc
     9361 gacagcgatt cagcgcatgc cggcatggga ccggggaccg agaacgggta cgatgatggg
     9421 tcctcgaacg ccgacaatgt gaaggtgaac gagaacggcg aacttgaact ggacaacggg
     9481 tccgactcgg tccagacgct cctgcgatat gtctgggtcc ccaattcggc ggacggcacc
     9541 atctccaaga tagactcctt cactcaagtc gaggtggctc gctatcatgc cggtctctcg
     9601 gccctttcca atccttcgcg caccagcgtc gatctggcgg ggaatatgtt cgtcggcaac
     9661 cgtgacgata gtaccgtgac caagatagcg gggcatccgg aatactgcgt cgaccgcgac
     9721 ctgagcggca ccatcgagac ctcgaccggc ccgaccgatg tcctgccgcg cggcgtcgac
     9781 ggccgatcga ccgacgaatg cgtcgtctgg acccgactct tcgacgcatc cgacctcaac
     9841 agtccctacg cctgcggcgg catccgcggc gtcgcggcga cacctgaaac gggggcgaac
     9901 ttcgagtaca ccggccacct gatgctcgga tgctacctgg gcaacccttt ttcctaccgg
     9961 ctgaatggca acaccggcga tatcatgcag atattctcga tgaccagcac gaccgatcag
    10021 acctgtcgcc cctacggcta tgtcctcgac aagtacggtc gcaactgggt ctcgtgcagg
    10081 tacatccccg agtggcagac accggtcacc ggcggtctcg cgtggttcga catcaacgat
    10141 gtgaccgaac agatacacta tgtgcccgac atacccgcct atggcggtgc gggaggttac
    10201 tacggcattg ccatagataa agatgacaac atctgggtca cctcctacac cgacggtttc
    10261 gtacaccgct acacccccga cgattccccc ttcgccgaca acgggacctg gacctccctt
    10321 cagatctcga ccgctcacgg cttccgcggc atcgccgccg acgattccgg attcatctgg
    10381 accatctcga ccgaagaggg caccgggatc gattcgcgta tatacatgat agactcctcg
    10441 atctaccccg atccctcatc gatacacggt ccctatatcc tcggtgacga cacgacccat
    10501 ccgttccaag ggaccggcgt cgggatcgat ttcaaaggca acgtctgggg catgtcgcgc
    10561 tgtaacgccg ggcaaggata cgtgacctat ctcccggtcg accggtcgac cccggtcccg
    10621 accgtcgtcg aggcggggaa aaagatcatc gcggtcggta acggccccta tgtctactcc
    10681 gacatgatcg ggtacaacct caagaatttc gcatcgaaag agggttggtt ccgccacata
    10741 ttcgagatct gcgcctcttc tctttcgacc gattggcaac agatatactg ggacgccgag
    10801 gtcccagcga acacccgcgt catcatacgg gcgcgcacca gcaacgatat cctgaaacta
    10861 gcgaccgagc cgtggcagat catcgtcgag gtgccttcgg acctttcccc gaaggtgata
    10921 cctgacaccc tgcccgaggg ccattacata gaactcgagg tacgcctcta ttccaaggag
    10981 gtcggcattt cgccggtcat cggacagatc gccttcgact acgaatgcgt cgacccgtcg
    11041 tgaacctctt taccggagtt cttccctcat gagatactct cttgttttcc tcttgatgct
    11101 cgtaccgagc ttcgccggcg cggacgagct ctgcggtctg cctcccgaac tgctccctcc
    11161 gcccgatagc ggtcagaagt gttacgatcc gggaccagcg gatgaaacgg aaaaagaccc
    11221 caagcaggta gcgaacatcg aggaaaagaa aaaagaagaa gaggaaaaag aggaggagaa
    11281 ggacaaggat tcgggcatcg tcgccttccc gatagcgttc tatacctccg acaccagttt
    11341 cgggttcggc ggcaccgcga tactgttcaa gaaaagttac gagaccgagg aggagaaacg
    11401 gtccgattcg ttcacgacgg tcatcttcta cacgctgaac aatcagatac tgaacgccaa
    11461 cgccggcacc ctatatatgg acaatgggaa tttcaaagcg aacgccgtgt tcgtggtctc
    11521 gaagtacccc gacgaattct acggcatcgg gcccgacagc ggctgggatg acggtgaaaa
    11581 gtacaccccg ctcacgataa ggggagagat ctcgttctcg gcacggcttt gggaaaagat
    11641 atacggcggg ctgaccgtcc tgcagggata ccacgaactg atgaaatacg accggaacgg
    11701 tgtgctcgcc gattatctgc gcgacaaccg gaccgacggc ttcttctcgg ggctcggcct
    11761 cctgttcacc cgcgacagcc gagacaactc gctgtacccc cgcaccggcg ccgtcacgac
    11821 cttttccgtt ctctctttca accggctcct gctgagcgac tacaccttca gcaccctcct
    11881 attcgatcac cggcagtatc actccctgcc gctcgacagc gtgatcgcat ggcaactgca
    11941 tttcaattcg gtgctcaccg gcaaaccacc gctcccctac ctcccggcgc tcggcagcca
    12001 gaacctgatg cgcggctatc cacagaaccg ttaccgcgac cggaatttca tggcggcgca
    12061 ggtcgaatgg cgcgtcccta tctggtggcg gttcggcgcg gtcgtcttcg ccggactcgg
    12121 caccgttaag ggttcgatcg acgactacct gttcaccgac ccgaaattcg ccgccggcgc
    12181 ggggctccgc atcacgctct cgaagaagaa caacatcaac ttccggttcg atttcggcat
    12241 gtcggctgaa cggccctacc tgaaacttta cttcaatctg ctggaagcat tctaaagaaa
    12301 agggcggccc gcaggccgcc cccaaaagcg tagagccgtg aagcgatctt acttggtcgc
    12361 ctcgtcgagt tcgttgaagg ccttgttggc gcgctccacg acggccgcgc ggaccttttc
    12421 gtcgagctgt tgcatctggt tgatggctcc cttgaacttc tcgacatcca tgcacatctg
    12481 gacatacagc gtgccggtct tcgagatcca ggtgttcttc tgctcggtgc cgctgagggt
    12541 ggtgtcggtg atctgcttgg acacatcctc aatgtgctgt tcgtcgttcg ccgcctgacc
    12601 gaaggcctcg ccgccggtgg tggtcgactg gtaatccttc agcatcgatt tcaccttgac
    12661 ctgaagcgcg cgcgcgatcg cggtgcgtcc tctgccctgc gccgcagtgc gggcgaggga
    12721 gacattgcgg gtaccggtcg ccgagccgac gccgcagata agaccgctac cgccagccga
    12781 ttcggcaagc acccactcgg gcgcgccatc aagttcgttc tgcatcgcgt tcgccggcgc
    12841 cttctcgctg gaaccacagg aagcgatgaa aagagccacg ccgaggatcg ccataaggac
    12901 caacatcttc ttcattctac cactcctttc cattatggtt gataaaaccg ccctagcgcc
    12961 atgttgcaca acgccttata tcgcatcgac ctcatcaagt caaatttttc tccggcaccg
    13021 cgaacaggcg gtacagaccc gtacaagagg tggttattcg aagatatttc gaggtctttg
    13081 atttgtcaaa agaaagcggt cgccgaaagt tttagcccca gactgtcggg cgaggcggcc
    13141 ccttcggcga aattcataaa gaaatagacc gccggacgga catacccgcc gatgagatac
    13201 cccccgccga cctccagccg cgtctggaag agaccgtatt cccactccgg cggcctgatc
    13261 atcatctcat cgaacttgag gtgtagccgc gcagaaagtt ccagcccgac gatagggtga
    13321 ccctgtatct cgaaaaaggg ccagaccggc agataccatt ttttgcccgg atgtttctcg
    13381 atgagtagca caccgcccgt cgagagcgcc agtttgcgat cgaacaacca ttgccgcacc
    13441 tgcagggcga tcgaacggct cacatagcgc atcggcgcaa cggcatactc gtgatagaac
    13501 cagccgaaca gatcggtatc agtcgtgaaa ttccaacgcc cttttatgta gagcgccaat
    13561 cggtccttat cgttgagcga cccagtcttt tctctcttgt aatagagggc ataggagag
//
LOCUS       DAOQUE010000014        13415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_5197_length_13415_cov_4.571482, whole genome shotgun sequence.
ACCESSION   DAOQUE010000014 DAOQUE010000000
VERSION     DAOQUE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13415
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5197_length_13415_cov_4.571482"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PKH10_00875"
     CDS             <1..756
                     /locus_tag="PKH10_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNT26705.1"
                     /translation="FVSGRSTLTAKGATSFPLRDILSAADSFTVLMWAALLATLTAGG
                     LVLLRRTLDTDKLAAAWLKGLQMMLPALMILTLAWTLGSVTKDLGTAEFIAQGLSGVL
                     SAGLLPAAVFIAAAVTSFATGTSWGTMAILFPTVIPLAAGIAGAQGMETAALNELAIL
                     TVGAVLTGSIFGDHCSPVSDTTVMSAMYSGLPLMDHVRTQLPYALLYGTIAVVLGYLP
                     AGFGLNPFLCLVLQIAAAVAILGYIGKRTTEQE"
     gene            756..1583
                     /locus_tag="PKH10_00880"
     CDS             756..1583
                     /locus_tag="PKH10_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNT26706.1"
                     /translation="MDKISLQRFRREILDHFKKHGRSFLWRLTIDPYEILVSEVMLQQ
                     TQVDRVSRYFPRFIERFPDLASLAAAPLREVLALWRGLGYNRRALFLKRVAEEAVRHH
                     DGMLPRTVKELITLPGIGPYTARAVLTFAFNEPHAFLETNIRAAYIHHFFAGREKVAD
                     REILPLLEKTIDRADPRRWYYALMDYGAHLKKRVPNPTRRSATHAKQSRFEGSDRQVR
                     GAILRALGTGATTRSPLLAAVRQDTGNDVDEERLSRILSGLCDEGMIKRSGGWYRIP"
     gene            complement(1584..2063)
                     /gene="ssb"
                     /locus_tag="PKH10_00885"
     CDS             complement(1584..2063)
                     /gene="ssb"
                     /locus_tag="PKH10_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNT26707.1"
                     /translation="MAYNSFNKIFILGRLGKAPELRYSTAGNPITKFSVATNERLKGT
                     GGQAEEHTEWHRAVVFGAQAENCSKFLKSGSLVFIEGKLRTRKYTGKDNEEKFFTEVI
                     CDTIHFLDSLGQGGEPQKGAERTYQKNDASPKSKEDEFNSAVSDALKEAPEEEDLPF"
     gene            complement(2068..3225)
                     /locus_tag="PKH10_00890"
     CDS             complement(2068..3225)
                     /locus_tag="PKH10_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26708.1"
                     /translation="MKIKIILLSLISILLVFGAAYHLLVNRTERQLKSALEENVSRAT
                     ALYKYINAAETLNRIREAEEIASRKEMLDVFDPAALKTNRAEVERNVQVELNVVNKFA
                     DDADILFVTDTEGLVVAKNLDNTLNGVSFRENLLISNAIAGRSDEDIFVILNKLVKVV
                     SVPIRKNGQIIGTYNSADIIDSEMAKEDFSNFAERASEEKGEAPLRLAFVAKGKVLGS
                     TMPPDLHEALKKFVEGEQQIVEQSLKEETRQHAFTLTLAGERYYANIAAHQHLGQEKQ
                     VCYLFMNSIDRNLEKLRTSATYFSYIVLIIAILSLLFAIFIEEQAMAPINRFMEGMIE
                     LIHGNRAFRFNNEAEGLEGQLNQNANMMIAALLGERLPEEKSAPEKRGREV"
     gene            complement(3215..3835)
                     /gene="purN"
                     /locus_tag="PKH10_00895"
     CDS             complement(3215..3835)
                     /gene="purN"
                     /locus_tag="PKH10_00895"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNT26709.1"
                     /translation="MKVAILISGTGSNMEAVIRARQQGRLPGVEAIPIVVSDTPDAPG
                     LEKARRLGVATAVVARRPKEPRESHEERLLGALAPHGAGLLVLAGFMRVLSPFFLSRF
                     PGRVINIHPSLLPAFPGLDAQKQAYDHGVKITGCTVHFVDETLDGGPILLQRAVERKD
                     EDTAEDLRLRILKEEHELLSEAIAIVSEGRASIVNRYVKVERSHEN"
     gene            complement(3832..4062)
                     /locus_tag="PKH10_00900"
     CDS             complement(3832..4062)
                     /locus_tag="PKH10_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26710.1"
                     /translation="MYGEGCIVTLQPDKNGLRPRDPDIRLLPLDKQKVRYFLQVNGGE
                     KKQYLFAPSDRKGEVIVPKSGDTISLTEVREP"
     gene            complement(4022..4768)
                     /locus_tag="PKH10_00905"
     CDS             complement(4022..4768)
                     /locus_tag="PKH10_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26711.1"
                     /translation="MFRVCVLLLVVLTTAPLFAKSDHAVLKLCFEKEGSPFLISTKDR
                     KLAEQERFQQIKESALWGRKMLVLLDRQKIFSFKPASDSCIVISKIPAGTHTVAVDFK
                     GASYTALVPKGEVTKKFKADLLFEAVLAVEEGPTAQVELSQEQAQISLFQTVDQSAVP
                     DCKERCTIPAEVPVYFMTRAEDEKIECPVQFELVVPHEREKSLNCYDAGRITADLRAL
                     VKTNNIKCRANLEYAFFRVRRRMHRHPSAR"
     gene            4906..5916
                     /gene="aroF"
                     /locus_tag="PKH10_00910"
     CDS             4906..5916
                     /gene="aroF"
                     /locus_tag="PKH10_00910"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNT26712.1"
                     /translation="MIIVLKRESKGTSEEIRHRLMAGGFQFNVLEGVKYTVIPVVGDL
                     TSSDINRFRSVPGVEKVLRISMPYYMVTKEYRRRTVIDLGNGVKLGDGFQFIAGPSSV
                     ESEESFDRIAAFLSGLGVKLIRGGTYKMRSSPYSFEGLGDEGLQIMRTVANRYGLKTV
                     SEIVDLRSLDQFLEQVDVIQVGARNMYNFPLLREIGRSGKPVLLKRAPSARMEDLLLS
                     AEHIMLEGNENIILCERGSLSFDEVTRSAVNIAAVPVMKDLSHLPVLLDPSHGVGVAR
                     YIPAVSEAAPLLGADGLLIEVHFDPSNAISDGFQSLSFEEFSRLYGRIGKTLSCLSCS
                     SC"
     gene            5910..7055
                     /locus_tag="PKH10_00915"
     CDS             5910..7055
                     /locus_tag="PKH10_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNT26713.1"
                     /translation="MLIPKFRSLPSRVLSLIETLLLFSLGLFLYFFVLTEPPPIEEVV
                     RLPSAATNDPEEETPGDEPDTASSPTALRERYDFDGGELLFDDAVEPALVALIDAAGS
                     RLRAVTYTVEVCPAVASIERAAARGVEVKVVAGRSQFKRRPSFPLTEFHPPKGILHEK
                     FVVADGRTVFLSSRNLSGGQSKNAAILFSDAPKLAALLTEEFDALKEMRIEKRCEKGC
                     RVEFGTLFFLPGTGCRAAKEDILSARIGVDVAMYTLTLGIPPLTGVKKLLKKGKKVRF
                     LVDDWSGEPGEASVNSRAARYLESLGATVLFDRLLDGRGEPMNFHHKFAVVDGGAATV
                     FGSMNWTKAGCYRNREVLFLSRDREIGAAFSSYFEDILQAVPPPPVR"
     gene            complement(7048..7659)
                     /locus_tag="PKH10_00920"
     CDS             complement(7048..7659)
                     /locus_tag="PKH10_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNT26714.1"
                     /translation="MEDSFLTIATATGPYEIKEKGSRFLSFVTPAVSEGEVLSFVSAL
                     RKRYHDATHVCWAYRLGDGEERASRYSDDGEPSGSAGLPLYQELARRGLFNVACAVVR
                     YFGGVKLGTGGLCRAYGGAARAALEETPPHTETVTVTVTVAAPFDLLGTVLHVVKRYP
                     GASVSAPAYREEGVVVEARIPRSKVAPFKDELTERSGGRVLLT"
     gene            complement(7660..7947)
                     /locus_tag="PKH10_00925"
     CDS             complement(7660..7947)
                     /locus_tag="PKH10_00925"
                     /inference="COORDINATES: protein motif:HMM:NF021188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HNT26715.1"
                     /translation="MPQLRIEHRTERTKEDIWRWVEEHIERELCAKVPREEFRVTESA
                     RDCAFTVQGKNVVAQVQVSDNLLAIELKIPLLFVPFQSRIEAGIRSALQAL"
     gene            complement(7970..8680)
                     /gene="rnc"
                     /locus_tag="PKH10_00930"
     CDS             complement(7970..8680)
                     /gene="rnc"
                     /locus_tag="PKH10_00930"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNT26716.1"
                     /translation="MALDTRLVKELEAALDCRLADTALLEEAFVHPSLDTAGSANYQR
                     LEFLGDEVAGLAVSTLLYRRFPGAEEGQLSRARAYLTNEGSFADIARTLGLGDLLILG
                     KGEEKTAGREKDSILADVFESLMAVIFLSNGWETALKVAERIFIPRFGAATEVEGLLR
                     HIDQDHKSHLQELAQEAGQPLPSYTLAGKQGPDHESLFTVECRALGLTAVGTGRNRKA
                     AEQEAARHLLTLLKGQDR"
     gene            complement(8670..9440)
                     /gene="rlmB"
                     /locus_tag="PKH10_00935"
     CDS             complement(8670..9440)
                     /gene="rlmB"
                     /locus_tag="PKH10_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNT26717.1"
                     /translation="MSKPRWEYVYGINPCFEILRGGRRKVHEAFMADNAGDNPRIKKL
                     VRYLGDKGIVVTPVGRDRLFQLSRSTEHQGAVIRCEPYPYVELDDVFTADKILLLDNV
                     EDPHNVGAILRSAEIFGFHDILLPLKGVPEIYPSIVKVSAGATEHLRIVRSGNANAYV
                     KKAKSEGRMIIALDEEGAIDLSEAVEKRRGPILLVIGGEGKSVGQFILMNADIVAKIP
                     QAGRINSLNASVAAGIAMYAFGLATAKPQTKPGADHGA"
     gene            complement(9551..10249)
                     /gene="pyrF"
                     /locus_tag="PKH10_00940"
     CDS             complement(9551..10249)
                     /gene="pyrF"
                     /locus_tag="PKH10_00940"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNT26718.1"
                     /translation="MEKGKVFIALDVADRREALAIVAELKGLGAGWKIGKQLFTQEGP
                     ALVKEIVAMGEDVFLDLKYHDIPHTVAMAGVAAAKLGVKMFNVHAAGGRMMMAETRKA
                     LDTLPDRPLVLAVTVLTSLSAEDLAEVGYHASPAQQVARFAKLAEEAGMDGVVASPLE
                     IEIIRSVTRPSFKILTPGIRPAGGSQDDQKRIATPAAAIAAGADYLVIGRPVTQAVDR
                     KKAFLDILEEIKNG"
     gene            complement(10337..11170)
                     /locus_tag="PKH10_00945"
     CDS             complement(10337..11170)
                     /locus_tag="PKH10_00945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT26719.1"
                     /translation="MPHFSIVIPSLNQGGFIGDALRSIAGQNYPAVEVIIRDGGSTDD
                     TAAVVRGFGELVTTFVSERDNGQSDALNKGFAQATGDIFGWMNADDHYLPGAFAEAAR
                     IFEANPEITVVYGNWVTIDATGTEIAKHYALTARRPHFSYENLDAYNQSLFWRRSAHE
                     RFGRFDENLHRLMDDDLILALLLNEGPERFARTDTFLGAFRVHESQKTPDAAIDEKHR
                     AEQEYLDRKYRFAPAGSTRGKIYRLRYRWHQFFDSLVAGGLYYTIGKAREGLRKRRGL
                     W"
     gene            complement(11178..11456)
                     /locus_tag="PKH10_00950"
     CDS             complement(11178..11456)
                     /locus_tag="PKH10_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26720.1"
                     /translation="MKRILSAIIVLCAFTAFAGDYGKCSFDSNCHPGVKCNSGVCADA
                     AGSKCGFDSDCGGKGAKCNSGKCSNAPDGKCSFDSECPGKKCNSGKCK"
     gene            complement(11520..>13415)
                     /locus_tag="PKH10_00955"
     CDS             complement(11520..>13415)
                     /locus_tag="PKH10_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26721.1"
                     /translation="YAGGWFSGGILKWDGVTWSSLGTGVDADGHIETMVFDDDGDLYI
                     GGHFTKAGDVAAIRVAKWDGIVWSALGDGLGDPSALNQSVHDLIFDKNGVLYAAGYFT
                     VGGEKIRLAKWDGASWSKKEAGIMEGSLSVLDIDNDNNLYIAGSFSLITTGGATANNI
                     AKWDGSSWCVLGDGVANVNGDNVRSLIIDDSNVLYAGGYFTTAGDVAATSIAKWDGTA
                     WSALFDDGGGINNAVTALTVDQNGILYVGGEFTAVNDMTANHIAKWDGISWTLLGNGV
                     SSIESMGIDGNNNLYVGGNFTTASDVSANHIAKWDGAAWYPLGNGVSDDALALTFDKN
                     NNLYVGGKFPLADDILVNRIAKWNGSTWSSLGGGVGDYRNVYALAADMFGHIYAGGGF
                     TKIGGISANYIAKWDGSSWSALGGGISGKYYVTVLAIDSNGMLYAGGDFTQAGGQPAN
                     NIARWNGNAWYALGEGLSGSVYAIALDANNNLIVGGNFTKAGDVAVNNIAKWNGSSWS
                     ALGGGVSGQVLALAVDGKQNLYVGGAFLQAGDKTSPYLAKYLIPDEEVFLPDDEVNDD
                     DMSDEEVFLPDDEVNDDDMSDDIDIVFSDSTDTATTDTFQPDEQTPVDADTTMPNSDG
                     CGCSILF"
BASE COUNT         2485 a         4191 c         3844 g         2895 t
ORIGIN      
        1 ttcgtcagcg gccgctcgac cctcacggcg aagggggcga cctccttccc gctccgcgat
       61 atcctgtcgg cggccgattc gttcacggtg ctcatgtggg cggcgctcct cgcgacgctc
      121 accgccggcg gactcgttct tctgcgccga acgctcgaca ccgacaaact cgccgcggcg
      181 tggctgaagg gtctccagat gatgcttccc gccctcatga tattgacgct cgcgtggacg
      241 ctcggttcgg tgaccaagga tctgggcaca gccgaattca tagcgcaggg gctttcgggc
      301 gtgctgtcgg cggggctgct ccccgccgcg gtatttatcg ctgccgccgt cacctcgttc
      361 gcgaccggca cctcgtgggg gaccatggcg atactcttcc cgaccgtgat accgctcgcc
      421 gccgggatcg ccggggcgca gggcatggag accgcggctc tgaacgaact ggcgatactc
      481 accgtcggcg cggtgctgac cggctcgatc ttcggcgacc actgctcacc ggtgtcggac
      541 accaccgtca tgtcggcgat gtattcgggc ctgccgctca tggaccatgt ccggacgcaa
      601 ctcccttatg cgcttctcta cggcaccatc gcggtcgtgc tcggttacct tcccgccggt
      661 ttcggcctca atccgttcct ttgcctcgtg ctccagatag cagcggcggt cgcgatactc
      721 gggtatatcg gcaagcggac gaccgaacag gagtgatgga caagatatcc ctccagcggt
      781 tccgtcgaga gatcctcgac catttcaaaa aacacgggcg ctcctttctc tggcggctca
      841 cgattgaccc ctacgagata ctggtctccg aggtgatgct ccagcagacg caggtcgacc
      901 gggtctcgcg ttatttcccc cgattcatcg aacgattccc ggacctcgcc tcgctcgccg
      961 cggcgccgct ccgcgaggtg ctcgcgctct ggcggggact cggctacaac cggcgcgccc
     1021 tctttctcaa acgggtggcc gaagaggcgg tacggcatca cgatggcatg ctcccgcgca
     1081 ccgtgaagga acttatcacg ctccccggca tcggccccta cacggcccgg gcggtactta
     1141 ccttcgcctt caacgaacca cacgccttcc tcgagaccaa catacgagcc gcctacatcc
     1201 atcatttttt tgcgggccgt gagaaagtgg ccgaccggga gatcctgccg ctgctcgaaa
     1261 aaacgatcga ccgcgccgac ccgcgccgct ggtactatgc actcatggac tacggcgctc
     1321 acctcaaaaa aagagtgccc aacccgacgc gccggtccgc gacccatgcg aaacagtcgc
     1381 ggttcgaggg aagcgaccgt caggtacgcg gcgcgatact ccgggcgctc ggcacgggcg
     1441 caacgacgcg gtcccccctg ctggccgcgg tacggcaaga caccgggaac gatgtcgacg
     1501 aggagcggct gtcgcgcata ctctcgggac tgtgtgacga agggatgatc aaaagatcgg
     1561 gcgggtggta ccggataccg tgatcagaac gggagatcct cttcctccgg cgcctctttc
     1621 agggcgtcgg aaacggcact gttgaattcg tcctctttgc ttttggggct cgcgtcattt
     1681 ttctgatagg tgcgttcagc gcccttttgc ggttcgcccc cctgaccgag ggagtcaagg
     1741 aaatgtatcg tgtcgcagat gacctcggtg aagaatttct cttcgttatc cttaccagtg
     1801 tacttacggg tgcggagttt cccctctata aagacgagcg agcccgactt gaggaacttc
     1861 gagcagttct cggcctgcgc accgaacacc accgcgcggt gccattcggt atgttcctcg
     1921 gcctgtccgc cggtcccctt gaggcgttcg ttggtggcga cgctgaattt cgttatcggg
     1981 ttccccgcgg tcgaatagcg tagttcgggc gccttgccga gccgaccgag gatgaatatc
     2041 ttgttgaaac tattgtacgc catctgtcta cacctccctt cctcttttct ccggcgcgct
     2101 cttttcttcg gggagccgct cgcccaacaa cgccgcgatc atcatgttcg cgttctggtt
     2161 caactgcccc tcgagtccct ccgcctcgtt gttgaaccgg aaggcgcgat tgccatgaat
     2221 gagctctatc ataccctcca tgaaacggtt tatcggcgcc atggcctgtt cttcgatgaa
     2281 gatagcaaag agcagggaaa gaatggctat tatcaggacg atgtaagaga aataggtggc
     2341 gctcgtacgg agcttctcaa gattccggtc gatggagttc atgaaaagat agcacacctg
     2401 tttttcctga ccgagatgct ggtgcgcggc gatgttcgca tagtatcgct cgccggcgag
     2461 tgtcaaggtg aaggcatgtt gccgcgtctc ctccttgagt gattgttcca ctatctgttg
     2521 ttcgccctcg acgaattttt tgagcgcctc gtgcaggtcg ggcggcatgg tcgaaccgag
     2581 caccttccct ttcgccacga aggcgaggcg gagcggcgcc tcccccttct cctcgctcgc
     2641 ccgttcggcg aagttgctga aatcctcctt ggccatctcg gagtcgatga tgtcggcgga
     2701 attgtaggtg ccgatgatct gaccgttctt gcggataggc accgacacga ccttcaccag
     2761 tttgttgagg atgacgaaga tgtcctcgtc gctcctgccc gctatcgcgt tgctgatgag
     2821 caggttctcc ctgaaactca ccccgttaag cgtgttgtcg agattctttg cgaccacgag
     2881 cccctcggtg tcggtcacga agaggatgtc ggcgtcatcg gcgaacttgt tgacgacatt
     2941 gagttcgacc tgcacattcc gctccacctc ggcgcggttc gtcttcagcg cggccggatc
     3001 gaacacatcg agcatctcct tgcgcgaggc gatctcctcc gcctcccgga tgcgattgag
     3061 cgtctcggcg gcgttgatgt acttgtagag cgccgtggcg cgggacacat tctcctccag
     3121 tgcgcttttc aactgccgtt cggtcctgtt cacgagcaga tggtaggccg ccccgaacac
     3181 aagaaggatc gagatcagcg aaagcaggat gatcttaatt ttcatggctc ctctccactt
     3241 tgacatatcg gttgacgatg ctggctcttc cttcgctcac gatagctatc gcctcgctga
     3301 gaagttcgtg ctcttctttc agtatgcgca ggcgcaggtc ctctgccgtg tcctcgtcct
     3361 ttcgttccac ggcgcgttgc agaagtatgg gcccgccgtc gagcgtctcg tccacgaaat
     3421 gcacggtgca tcccgttatc tttaccccgt ggtcatacgc ctgcttctgg gcgtcgaggc
     3481 cggggaacgc cgggagcagc gacgggtgaa tattgatgac ccgcccggga aaacgggaaa
     3541 gaaagaacgg cgacaggacc cgcatgaacc cggcgaggac cagtagcccg gccccgtgcg
     3601 gagcgagcgc tcccagcaga cgttcctcat gcgactcgcg cggctccttg ggccgacgcg
     3661 ccacgacggc ggtcgcgacc ccgagccgtc gggccttttc cagccccggc gcatcagggg
     3721 tgtcggagac aacgatcggg atcgcctcga cgcccggcag cctgccctgt tgccgcgccc
     3781 gtatcaccgc ttccatgttg gagccggtgc cggagatgag tatcgcgacc ttcatggttc
     3841 gcggacctct gtcagagaga tggtgtctcc gctcttgggg acgatgacct cgcccttccg
     3901 gtccgaaggg gcgaataggt attgcttctt ctccccgccg ttgacctgca gaaaatagcg
     3961 gaccttctgc ttgtcgagcg gcaggagccg tatgtcggga tcgcggggcc ggagtccgtt
     4021 cttatcgggc tgaagggtga cgatgcatcc ttcgccgtac acggaagaag gcgtactcga
     4081 ggttcgcgcg gcatttgatg ttgttcgtct tgaccaaggc gcgcagatcc gccgttatgc
     4141 gaccggcgtc gtagcagttg agcgactttt cccgctcgtg cggcacgacc agttcgaact
     4201 ggacggggca ctctatcttt tcgtcctcgg cacgtgtcat gaaatagacc ggcacctcgg
     4261 ccggtatcgt gcatcgttcc ttgcaatcgg gcaccgcact ctgatccacc gtctggaaca
     4321 gcgagatctg cgcctgttcc tgcgatagct ccacctgtgc cgtcggccct tcctcgaccg
     4381 caaggaccgc ttcgaacaac aggtcggcct tgaatttctt ggtcacctcc cccttcggca
     4441 ccaacgcggt atacgaggcc cctttgaagt cgaccgccac ggtatgggtg ccggccggta
     4501 tcttcgagat gacgatgcag gaatcgctcg ccggcttgaa ggagaatatc ttctgacggt
     4561 ctaggagcac cagcatcttg cgtccccaga gggccgattc cttgatctgt tggaatcgtt
     4621 cctgctcagc cagtttacgg tccttggtcg atatcaggaa cggggagcct tccttctcga
     4681 aacagagttt caggacggca tggtcgcttt tcgcgaacag tggcgctgtc gtcagcacaa
     4741 ccagcaagag tacacagacg cggaacatcc cctacctctg gaaaaaagaa agggcaccct
     4801 acagggtggc gattatagca ggtcgccttt ccttgtaaat aaaatttgtc atttttaggc
     4861 ggagcgttat actcctcacg tgtcacggca cggagaccgg gaaacatgat catcgtcctg
     4921 aaacgcgaaa gcaaggggac cagcgaagag atacggcatc gactcatggc cggcgggttc
     4981 cagttcaatg tcctcgaagg ggtcaaatac accgtcatcc cggtcgtagg agatctgacc
     5041 tcgagcgata tcaaccgctt ccggtcggtc cccggggtcg agaaggtcct gcgcatctcc
     5101 atgccctact acatggtcac caaggaatac cgccggcgga cggtcatcga tctcggcaac
     5161 ggcgtcaagc tcggcgacgg ctttcagttc atcgccggcc cctccagcgt cgaaagcgaa
     5221 gagtcgttcg accgcatcgc ggcgttcctg tcagggctcg gggtcaaact gatccgcggc
     5281 ggcacctaca agatgcgctc ctctccgtac agtttcgagg ggttgggcga tgaggggttg
     5341 cagatcatgc ggacggtcgc gaaccggtac ggattgaaga cggtcagcga gatcgtggac
     5401 ctgcgcagtc tcgaccagtt cctcgaacag gtcgatgtca tacaggtcgg cgcccgcaat
     5461 atgtacaact tccccctgct ccgcgagatc gggcgttcgg gcaagccggt cctgctcaaa
     5521 cgggcgccgt cggcccgtat ggaggatctc ctgctctcgg ccgaacacat catgctcgag
     5581 gggaacgaga acatcatcct atgcgaacgc ggctcgctct cgttcgacga ggtgacccgg
     5641 agcgccgtga acatcgcggc ggtaccggtc atgaaagatc tttcccatct gccggtcctg
     5701 ctcgatccct cgcacggggt cggggtcgcc cgttacatcc cggcggtcag cgaggcggct
     5761 cccctgctcg gcgccgacgg cctgctcatc gaggtccatt tcgacccctc gaacgccatt
     5821 tccgacgggt tccagtccct gtccttcgag gagttctcgc ggctgtatgg ccgcataggt
     5881 aagacattgt cctgcctttc ctgttcctct tgctgatacc gaagttccgc tcgcttcctt
     5941 ctcgcgtcct ctcgctgatc gagaccctgc ttctgttctc gttaggtctt ttcctttatt
     6001 ttttcgttct caccgagccg cccccgatcg aggaggtggt ccggctcccc tcagcggcca
     6061 cgaacgaccc ggaggaggaa accccgggcg acgaacccga taccgcctcc tctcccaccg
     6121 cgctcaggga acgctatgat ttcgacggcg gcgagttgct gttcgatgac gccgtggaac
     6181 cggcgctcgt cgcgctgatc gacgctgcgg ggtcgcgtct ccgggcggtc acctacaccg
     6241 tggaggtctg tcccgccgtc gcctcgatcg aacgggccgc ggcgcgcggt gtcgaggtca
     6301 aggtcgtcgc gggccgcagt cagttcaagc gacggccatc gttcccgctc accgagttcc
     6361 atccgccgaa ggggatactg cacgagaagt tcgtcgtggc cgacgggcgg acggtgttcc
     6421 tttcctcgcg caacctctcg ggcggccagt cgaagaacgc cgccatcctt ttttccgacg
     6481 cgccgaaact tgccgccctg ctcaccgagg agttcgacgc gctcaaagag atgcggatag
     6541 agaagcggtg tgaaaaggga tgccgcgtcg agttcggtac gctttttttt cttccaggca
     6601 cgggttgtcg cgcggcaaag gaggacatcc tctcggcccg catcggcgtc gatgtcgcga
     6661 tgtacacgct cacgctcggc atcccgccgc tcaccggcgt gaagaaactg ctcaaaaaag
     6721 ggaagaaggt ccggttcctc gtggacgact ggtcgggtga accgggagag gcgtcggtga
     6781 attctcgggc cgcccggtat ctcgagagtc tcggcgcgac ggtgttgttc gaccgactgc
     6841 tcgacgggcg tggcgaaccg atgaatttcc atcacaaatt cgccgttgtg gatggcggcg
     6901 ccgcgacggt cttcggctcg atgaactgga ccaaggcggg ctgttaccgc aaccgcgagg
     6961 tgttgttcct gagcagggat cgcgagatcg gcgcggcgtt ctcgtcgtac ttcgaggata
     7021 tcctgcaggc ggtaccgccg ccaccggtca ggtaaggagg acgcgacccc cgcttctctc
     7081 ggtaagttcg tccttgaacg gcgcgacctt cgacctgggg atccgcgcct cgacgacgac
     7141 cccctcctca cggtatgcgg gcgccgacac cgacgccccc gggtatcttt tcacgacatg
     7201 gagcaccgtt cccaacaggt cgaacggcgc cgccacggtt accgtcacgg tgaccgtttc
     7261 ggtgtggggc ggggtctcct cgagcgccgc gcgcgccgct ccgccatagg ccctgcagag
     7321 cccgccggtc cccagtttca ccccgccgaa atagcgtacg acggcgcagg cgacattgaa
     7381 aagtccccgc cgtgcgagtt cctgatacaa cggaagcccc gccgaccccg acggttcgcc
     7441 gtcgtcgctg taccgcgacg cgcgctcctc tccgtcgccg agccggtagg cccagcagac
     7501 atgggtggcg tcatggtatc gtttacgcag agccgaaacg aaggagagca cctccccctc
     7561 cgagacggca ggcgtcacga aggagaggaa ccgggaccct ttttctttta tctcgtacgg
     7621 gccggtggcg gtcgctatcg tgagaaaaga atcttccatc tacaacgctt ggagcgccga
     7681 gcggatgccc gcctctatcc gcgactggaa cgggacgaaa aggaggggta tcttgagttc
     7741 tatcgcgagc aggttgtcgc tcacctgtac ctgcgcgacg acattttttc cctgcacggt
     7801 gaaggcgcag tcgcgtgccg attcggtgac ccggaactcc tcgcgcggca ccttggcgca
     7861 gagttctctc tcgatgtgtt cctccaccca ccgccagata tcctctttcg tcctctcggt
     7921 ccggtgctct atcctcagtt gcggcatggc gttcctcctg tggtggttgt catcggtcct
     7981 gtcccttcag cagagtgagc agatggcggg ccgcttcctg ttcggccgcc ttgcggttgc
     8041 gaccggtccc taccgctgtc agcccgagcg cgcggcattc gacggtgaac agactctcgt
     8101 ggtcgggccc ctgtttgccg gccagcgtgt acgacgggag cggctggccg gcctcctgtg
     8161 cgagttcctg cagatggctt ttgtgatcct gatcgatatg gcggagcaat ccttcgacct
     8221 ccgtggcggc cccgaaccgc gggatgaata tccgttcggc gaccttcagc gccgtttccc
     8281 atccgttcga aaggaatatc accgccatga gcgactcgaa cacatcggcg aggatggagt
     8341 ccttctcgcg accggcggtc ttctcctccc ccttgccgag tatcagcagg tcgccgagcc
     8401 cgagggtccg ggcgatatcg gcgaacgatc cttcgttggt gagataggcg cgggcgcggg
     8461 aaagttgccc ctcctcggcg ccggggaacc gccggtacag gagcgtcgaa acggcgagcc
     8521 ccgccacctc gtcgccgagg aattcgagcc gctggtagtt cgcgctcccc gccgtgtcga
     8581 gcgagggatg gacgaatgcc tcctcaagga gcgccgtgtc ggcaagccga cagtcgagcg
     8641 ccgcttcgag ttccttcacc agccgtgtat caagcgccat ggtccgctcc cggcttcgtc
     8701 tgcggctttg ccgtggcaag cccgaaagcg tacatcgcga taccggcggc gaccgatgcg
     8761 ttcagcgaat tgatccgtcc cgcctgcggt atcttcgcga cgatatcggc gttcatgagg
     8821 atgaactgcc cgacgctctt cccctcgcca ccgatgacga gcaggatcgg cccgcgccgc
     8881 ttttcgaccg cttcgctcag gtcgatcgcc ccctcttcgt cgagcgcgat gatcatgcgc
     8941 ccttcgctct tcgccttttt gacataggcg ttggcgttcc cgctgcggac gatgcgcagg
     9001 tgttcggtcg ccccggcgct caccttgacg atggagggat atatctcggg gacccccttg
     9061 agcggcagaa ggatgtcatg aaagccgaat atctcggcgc ttcgcaatat cgcccccaca
     9121 ttgtgcggat cctcgacgtt gtcgagcagc agtatcttat cggcggtgaa aacatcgtcg
     9181 agctcgacat acgggtatgg ctcgcaacgg atgaccgccc cctgatgctc ggtcgaccgc
     9241 gacaattgga acagccggtc gcgtcccacc ggcgtcacga cgatcccctt gtcaccgagg
     9301 tagcggacca gttttttgat gcgcgggttg tcgccggcgt tatcggccat gaaggcctcg
     9361 tggacctttc gccgaccgcc ccgcaagatc tcgaagcagg gattgatgcc gtagacatat
     9421 tcccagcggg gcttactcat ttcccctccg ctggaacacc ctcatcccaa ccttctccct
     9481 agacagggcg aaggagtata aggtttttcc tctccctatc gagggagagg atgaaggtga
     9541 gggttttctg ttacccattc tttatctctt ccaaaatgtc gaggaacgcc ttcttcctgt
     9601 cgaccgcctg cgtgacgggc cgcccgatga caagatagtc ggcgcccgcc gcgatggccg
     9661 ccgcgggggt cgctatccgt ttctggtcgt cctgcgagcc gcccgccggg cgtatgccgg
     9721 gcgtgagtat cttgaacgat ggtctcgtca cgctgcggat gatctctatc tcgagcggcg
     9781 aggcgaccac gccatccatt cccgcttcct cggccagttt cgcgaaccgc gccacctgct
     9841 gggccggcga cgcatggtac ccgacctcgg cgagatcctc ggccgagaga ctcgtgagca
     9901 ccgtaacggc gagtacgagc ggccggtcgg gcagggtatc gagcgctttg cgggtctcgg
     9961 ccatcatcat gcggccgccc gcggcgtgga cattgaacat cttcaccccc aacttcgcgg
    10021 cggcaacgcc cgccatcgca acggtatggg ggatatcgtg atatttgagg tcgaggaaca
    10081 catcctcgcc catcgccact atctctttga ccagcgcggg tccttcctgc gtgaagagtt
    10141 gtttgccgat cttccagccg gcgccgagcc ccttaagttc ggcgacgatg gcgagcgctt
    10201 cgcgccggtc ggcgacatcg agcgcgatga acacttttcc tttttccatg cggtctcctc
    10261 gttcgttgtg ttctcttccg gcgcgattat atccgataac gccgcgaagg caaactgaac
    10321 cgcctttttt ttgcttttac cagagaccgc gccgtttgcg gagtccttcc cgtgccttgc
    10381 cgatcgtgta gtagagcccg ccggcgacga gcgaatcgaa gaactgatgc cagcggtagc
    10441 ggaggcggta gatctttcct cgtgtcgaac cggctggcgc aaaacggtat ttccggtcga
    10501 gatattcctg ctcggcgcgg tgcttctcat ctatcgccgc gtcgggcgtc ttctgcgact
    10561 catggacacg gaacgcgccg aggaaagtgt cggtgcgggc gaatcgctcc ggcccctcgt
    10621 tcagcaagag cgcgaggatg aggtcgtcgt ccatcagtcg gtggaggttc tcgtcgaacc
    10681 ggccgaatcg ttcgtgcgcg gagcgtcgcc agaaaagcga ttggttgtat gcgtcgaggt
    10741 tctcgtagga aaaatggggc cgccgcgccg tgagcgcgta gtgtttcgcg atctccgttc
    10801 ccgttgcgtc gatggtcacc cagttgccgt agaccaccgt gatctcgggg ttcgcttcga
    10861 agatgcgcgc cgcctcggcg aaggcgcccg gcaggtagtg atcgtcggcg ttcatccagc
    10921 cgaagatgtc tccggtcgcc tgcgcgaaac ctttgttgag ggcgtccgac tggccgttat
    10981 cgcgctccga gacgaatgtg gtgacgagtt cgccgaagcc tcgcacgacc gccgccgtgt
    11041 cgtcggtcga gccgccgtcg cggatgatca cctcgaccgc ggggtagttc tgacccgcga
    11101 tggagcggag ggcgtcgccg atgaagccgc cttggttcag cgacggtata acgatggaaa
    11161 aatggggcat cggtgcgtta cttacacttg cccgaattac acttcttccc ggggcattcg
    11221 ctgtcgaaac tgcatttgcc gtcgggtgcg ttggagcatt tgccgctgtt gcacttggcc
    11281 cctttgccgc cgcaatcgga atcgaatccg cacttggagc cggccgcatc ggcgcagacg
    11341 cccgagttgc acttgacgcc ggggtggcag ttggaatcga aactgcattt gccgtagtcg
    11401 cccgcgaagg cggtgaaggc gcacagcacg atgatcgccg aaaggatgcg tttcatgggt
    11461 tcctccctgt taattgaacg gtgaacagag ggagtataac gcgggaaaat taaaaagcac
    11521 taaaacagga tcgagcaccc gcagccgtca ctgttgggca ttgtggtatc ggcgtcgacc
    11581 ggtgtctgtt cgtcaggctg gaaggtgtcg gtggtggcgg tatcggtcga atcggaaaaa
    11641 acgatatcga tgtcgtcgga catgtcgtcg tcgtttacct cgtcgtcggg caaaaagacc
    11701 tcttcgtcgg acatgtcgtc gtcgtttacc tcgtcgtcgg gcaaaaagac ctcttcgtcg
    11761 ggtatcaggt attttgcgag ataaggactt gtcttgtcgc ccgcctggag aaaagcacca
    11821 cccacataaa gattttgctt cccatcaacg gcgagagcta acacctgacc agatactccg
    11881 ccacccagcg cactccacga tgagccattc catttggcga tattattcac cgcaacatcg
    11941 cccgccttag taaaatttcc ccccacaatg agattattgt tcgcgtcgag agcaatggca
    12001 taaacactac cgctcaaacc ttctcccaac gcataccatg cgttgccgtt ccatcgagca
    12061 atattgttag ccggttggcc tcccgcttgg gtgaaatcac cgccggcata gagcatgccg
    12121 ttactgtcaa tggcgaggac agtaacataa tatttcccgc tgatacctcc tcccagcgca
    12181 ctccatgaag aaccatccca tttcgcgata taatttgctg aaatgccgcc tattttggtg
    12241 aaaccgcctc ccgcataaat atggccaaac atgtctgccg caagagcata aacattccta
    12301 tagtccccca ctcctcctcc caaggaactc catgttgatc cattccactt agcgattcta
    12361 tttaccaaga tatcgtcagc cagagggaat ttcccgccaa cataaaggtt gttgttcttg
    12421 tcaaaagtca gggcaagagc atcatcactc acgccattcc ccaatggata ccatgcagcg
    12481 ccatcccatt tagcgatatg gtttgccgaa acatcgctcg ctgtggtaaa attccccccc
    12541 acatagagat tgttgttgcc gtcaattccc atggattcta tactggacac accgttcccc
    12601 agaagagtcc atgatattcc atcccatttg gcaatatggt ttgctgtcat atcgtttaca
    12661 gcagtgaact ctcccccgac atagagaata ccattctgat cgaccgtcag agcagtaacg
    12721 gcgttgttta taccacctcc atcatcaaaa agggcgctcc atgcagtgcc gtcccatttc
    12781 gcgatcgaag ttgccgctac atcacccgct gtggtaaagt atcctccagc ataaagaaca
    12841 ttggaatcgt ctatgatgag agatctaaca ttatccccat tcacatttgc aaccccatct
    12901 cctaatacac accacgacga gccatcccat ttcgcgatat tgtttgctgt tgccccacct
    12961 gttgtaatga gagagaaaga tcctgcgata tagagattat tgtcgttgtc tatatcaaga
    13021 acagaaagcg aaccttccat tatacctgct tcctttttac tccacgatgc accatcccac
    13081 ttcgcgagcc ttatcttctc acctccgaca gtgaaatatc cggcagcata aagcactcca
    13141 tttttgtcaa aaataagatc atgaacactt tgatttagag cggacggatc tcctaggcca
    13201 tcaccgagag cgctccatac gatgccgtcc catttggcga ctctaatagc tgctacatcc
    13261 cctgccttag tgaaatgtcc tccgatataa agatcgccat cgtcatcaaa aaccatggtt
    13321 tcaatatgac cgtcggcatc caccccggtg cccaaggaac tccatgtcac gccatcccac
    13381 ttcaatatgc cgccagagaa ccaaccaccg gcata
//
LOCUS       DAOQUE010000015        12284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_5849_length_12284_cov_5.430043, whole genome shotgun sequence.
ACCESSION   DAOQUE010000015 DAOQUE010000000
VERSION     DAOQUE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12284
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5849_length_12284_cov_5.430043"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..948
                     /locus_tag="PKH10_00960"
     CDS             118..948
                     /locus_tag="PKH10_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26722.1"
                     /translation="MSKPQTAAGYSVHQTELAERVLLEVWSRLGEYREHLVLVGGLAP
                     RYIVDQAKAKAIGASHCGTMDVDLGISIAVADLKIYRSIHDHLLEMGFLPGKNDRGHE
                     QRHSFVKEINAVPVSVDFLTATYDGPTNSLMREIEGTLSAIQVEGLGLAFREPLLCRI
                     EGELLSGGLTRETVCVCRAIPFVVLKALAFDKRREKKDAYDLVYVLRHATAGPRELAG
                     QTTAEERAQASFSHAIETMRNHFQSPTQNGPVHYASFTDRSSDATVAFAAVQDFLGGL
                     "
     gene            complement(961..1236)
                     /locus_tag="PKH10_00965"
     CDS             complement(961..1236)
                     /locus_tag="PKH10_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26723.1"
                     /translation="MNSLSCARRTDRTARVGRPKKAKKPGRKPAPKKRVSALQERFNK
                     EILTIRKYLNSRFSVKMIADMLEMPYQPLLALIKKHETLKGFVKKGR"
     gene            complement(1205..1969)
                     /locus_tag="PKH10_00970"
     CDS             complement(1205..1969)
                     /locus_tag="PKH10_00970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT26724.1"
                     /translation="MHDLSPRFWSIFFELYEALPRQGPGSRACAEKALGLCRDLPAAP
                     AILDLGCGVGGQTLHLAELTPGTIVALDNHAPSIDRLRATVAERGLSARIRPMVGDMG
                     NPGLPPKNFDLVWSEGALYHIGIENALRVCHGLLRAGGYLAFTDAVWRRKNPPPEVKA
                     SFDWDYPTMGRVADILATIAKSGFSLMGHFTLPDEAWWDDFYMPMELRIGELRAKYAE
                     DADAIAVLDQLAQEPAMHRRHSDCYAYEFFVVRPEN"
     gene            complement(1971..2753)
                     /locus_tag="PKH10_00975"
     CDS             complement(1971..2753)
                     /locus_tag="PKH10_00975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT26725.1"
                     /translation="MVREDENDPGQDIRLYRDLSEWYPLLTPVCDYAEEAAFYRRLFE
                     AHCQRSPQTMLELGSGAGHNAAYLKETLTCTLVDLAPAMLDLSRRLNSECEHIQGDMR
                     SVRLGRLFDCILVHDAVVYMTTRSDLAAAIATAFAHTAPGGVAMFQPDFVSENFTPGT
                     GSGGSDGNGRALRYIEWRWVPEAMTERYVTDMAYLMREEQGLFDVVHDRHLMGLFSRT
                     VWLDLIEAAGFKPLVVPFEHSDHIVTGSEVFLGLRPVTQGGK"
     gene            3134..3343
                     /locus_tag="PKH10_00980"
     CDS             3134..3343
                     /locus_tag="PKH10_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNT26726.1"
                     /translation="MAEDVYRLKGHACTVPRCQNDGDTLDHRIPWEHYKKGTCVDNLF
                     PMCSEHNSSKGEKDYFDWLRELDQR"
     gene            3415..3732
                     /locus_tag="PKH10_00985"
     CDS             3415..3732
                     /locus_tag="PKH10_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26727.1"
                     /translation="MLLNEKRSSELMIIQTFPPIGLFRAGGILEKEKEPSLIFRVFQE
                     FFPQIGIRTPDPTNDQLLSFILLAKMVIPRGSTVDHESNDAPKEKAGAVHKKRSVASG
                     LFR"
     gene            complement(3863..4699)
                     /locus_tag="PKH10_00990"
     CDS             complement(3863..4699)
                     /locus_tag="PKH10_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNT26728.1"
                     /translation="MKNFFILFLSLLPVAIFAADPIGDLETRQVALFERVAPCVVFIS
                     GAKNTLGSGFLISADGLILTNAHVVPEGKKVNVVLNDGKRFEGTVIERAKNNIDLALV
                     KIEAKGLPFLDISKDANIKVGSWAAAIGHGRGAVWTYNTGMISNVYIDKEGRAVIQTQ
                     IPINRGNSGGPVFDKDGVVVGVVTAEMTESNSINFALSVDLARNNLEGLSHLSRKLII
                     RAPEGLPIFVNGIMKGKGPQIALDVPAGTYEIFIVQNGEMKKVKIKYPQQKIVELPTA
                     SK"
     gene            complement(4717..5832)
                     /locus_tag="PKH10_00995"
     CDS             complement(4717..5832)
                     /locus_tag="PKH10_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26729.1"
                     /translation="MKKQLSFLLFFLTAVSLFASEEWERSFSPEIVATYLPSGKEKML
                     LVGAGERNPKMIAMIKALAQALKGTGKAAMLMDDSPLGDISAMDDASVVKKCSRFPVT
                     LIIVARYFKPSAVVTLYRPTGEVLNAFSVERGELLSPYETNIVEGGVSQSVLDAVSAA
                     VKKDEKEPETFYDRELLWFWEWKDGLSPGPDPDTDREPRIGKYKKALTWDVFFRKVGR
                     EDLAIEFDRREKAKKGLFIGGLSLAVVGAVFVVAGASVQTWKNDSRDYYRFNETTLTW
                     EKQAEDPLGPYFWSGLAMTITGTASWITGVLLNKNPATGKEVADMARAYNRELKKRTE
                     GSGAEEKTKEMSAEGIDLMIDPFALPSGGGINLRVSY"
     gene            complement(5829..6293)
                     /locus_tag="PKH10_01000"
     CDS             complement(5829..6293)
                     /locus_tag="PKH10_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26730.1"
                     /translation="MKKRKFLTMILLTIPLLSCEEYWPVRWGFKNASTEEITIALYAC
                     DGVCEDCGCKPDPVEQFSLAPGETRGLETNNFDRDDWDFEYICFGAYTETDPHFAGCG
                     KGCIYYQTSSSTARVCNYVSSFGDQEPFSTTVITKGSPVATEKKSEREEELK"
     gene            complement(6483..7235)
                     /locus_tag="PKH10_01005"
     CDS             complement(6483..7235)
                     /locus_tag="PKH10_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNT26731.1"
                     /translation="MSKIIAYVTKNRVGRPDEKQRAAMAAYMTAHELKPEIVEDAYSK
                     KKPWKKRTALSTTVENLKSGDTLIFPGKEYLGLDTQDRISLLAELLKKKVVVHLIAEG
                     IVLKGISEEGTTLLSLFALLLSPEKKSPGRKPGRPAKKKRGRPAKKKRGRPAKKKVGR
                     PPKKKVGRPPRRKVGRPKAKKKPGRKPARKKRVSSIQKQFNDQILTIRKYLVNRFSVK
                     TIAEMLEMPYQPLLGLIKKHETLKGFVKKKGK"
     gene            complement(7244..7522)
                     /locus_tag="PKH10_01010"
     CDS             complement(7244..7522)
                     /locus_tag="PKH10_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT26732.1"
                     /translation="MKKTDMVKELAARSGISKKAAEEFVNLMFDAMINALMREERIAI
                     RDFGHFTVRRIRACTSRNPKTGERIMIPERRFPFFKAGKDLLQRINNR"
     gene            complement(7542..8318)
                     /locus_tag="PKH10_01015"
     CDS             complement(7542..8318)
                     /locus_tag="PKH10_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3825 domain-containing protein"
                     /protein_id="HNT26733.1"
                     /translation="MKTPRSKLLEFAFFPEIDKRLEDIAILAEPEPWDYSDTLLKRFS
                     ILQNYLERTFEKLTEEENIAYTASREHVCFNTGLLTRNREDIFLFFQTNKHPAATSPY
                     FFMNAQKESDVLINRYFSGNLPYPADYFQQPELLICNPHLPLTIDYDHIIDENTNRFP
                     EPLRGNRYALRNLLKGAIEYTMKLVRNNYRTAVPQYFNGHIQLLLPLYLTDPHGPADL
                     ALVLYREEGGYVARTCLTMRMAYQNARIIVRPDAESWLKP"
     gene            complement(8325..8603)
                     /locus_tag="PKH10_01020"
     CDS             complement(8325..8603)
                     /locus_tag="PKH10_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT26734.1"
                     /translation="MLKSELIKIIAEKEGLRFREAETFVTLMFDALTAALQREERIEI
                     RGFGSFIVRHYGGYKGRDPRNGSPIKIPAKRLPHFKAGRDLLQRINNR"
     gene            8738..9028
                     /locus_tag="PKH10_01025"
     CDS             8738..9028
                     /locus_tag="PKH10_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNT26735.1"
                     /translation="MSLTKIELANLLYESMHVDKKQAEELVDLFFEELKKGLLKDGEL
                     KLSGFGNFQIKQKTKRRGRNPKTGKDLTIDARKVVIFKRSVVLKGRLNSAKG"
     gene            9146..9379
                     /locus_tag="PKH10_01030"
     CDS             9146..9379
                     /locus_tag="PKH10_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26736.1"
                     /translation="MGRRIRTTLSLDAGMVRELDDVSVELKQKKSHLIEQAWQFYLDY
                     LDVKIAEKRLAAVKNGKVRTIPAKKAFKELGLD"
     gene            9379..9645
                     /locus_tag="PKH10_01035"
     CDS             9379..9645
                     /locus_tag="PKH10_01035"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNT26737.1"
                     /translation="MVYAIEFEPQALKELGAIDRPAQRLIREKILLLGENYAALKNNI
                     TQLKGQKEPLFRLRVADYRVIFSKDDTRIIIFIVRVRHRREVYE"
     gene            complement(9841..11667)
                     /locus_tag="PKH10_01040"
     CDS             complement(9841..11667)
                     /locus_tag="PKH10_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26738.1"
                     /translation="MLLAGISYFTYTHFFGDRNPLRWKIGTTYEYEFVYAGETVNSHL
                     NPEGGTKDLSGKFDCTLRFSLTPVKRLPDGSFFSELAVVKVGACSFYFNGQDILENPE
                     AREQIFMGRHAGVVHDGFGAVSDVRFRKNEDPVFVGTMKLIMAYFQTALSGKKKPPAE
                     EWDQNGRYKVSYSYDGIGVGDTTVTKNIERYEELLAFPKQTKALEQRGTGSFRYQVKD
                     ALISEIKGTQDQTCAREGKDIFLLKVNLKMTLVDVRRRGFDLDGALARLNELRSSRLG
                     TVELNDEIKKKMYEQQARNLTVTEMLDTLRAYGTSGEIGDKKLFMWRMAGYLKRYPER
                     TTDLAPLFNDPTFSSDGRMLIMGALASVGHPEAQTVMRQLLAGDTAKNDRLYPLLLQN
                     ISYLDTPDPQTVSFAEKLYDDALATNKLRSTASLILGAVAGKTTRGGDGIRGAMLNNK
                     LVEELRKSATPESAERMLDAIGNVANSSNIAVAAEYKDSLNDRVRASVANAVRFTQTP
                     ESEQMLLDMLGDKSNLVKMNAVNTLSVYSLKKEHFEKIHENIKDGSLNPDQYLYLAAL
                     FRKNLAGNEDIIGNSLREMQKQQMPPQAASQIAGLLESLPKK"
     gene            complement(11761..>12284)
                     /locus_tag="PKH10_01045"
     CDS             complement(11761..>12284)
                     /locus_tag="PKH10_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26739.1"
                     /translation="GGGVKNLYDIKELGGLLYVEYQQSQLEDISALSEILNVKNIDLR
                     ENKLTNLAALSSLPNLESVRVINTPTLTTLGAQFTAPKLQTLNVSNCGLTSLNELSGL
                     PELTDIAAGSNYLTNISFITTLSKLEAIGIGGNCLPESEVQKYVNWWNTKYPESATTL
                     EEEIQYQDPQKCQ"
BASE COUNT         2685 a         3328 c         3343 g         2928 t
ORIGIN      
        1 ggctccgcgt ggtatccgat ccgcagttat atatcgacct ccgcaagatg cccggccggg
       61 caacggagca ggcggaagtg ctccgcaaca aatacctgaa ccgtgggatc ccggaagatg
      121 tctaagccgc aaactgccgc cggttattcc gttcatcaaa cggaattggc cgaacgggtc
      181 cttcttgaag tatggagtcg tctcggtgaa taccgggaac accttgtcct cgtgggcggg
      241 ctcgcgccgc ggtatatcgt tgaccaagca aaagccaaag cgataggcgc gtcccattgc
      301 ggcaccatgg atgtcgatct gggaataagc atcgctgtag ccgatctgaa gatatatcgc
      361 tccatccatg accatctcct cgagatgggg ttcctgcccg ggaagaacga tcgagggcat
      421 gaacagcggc attccttcgt aaaggagatc aacgcggtcc cggtgagtgt cgatttcctg
      481 accgccacct atgacggtcc cacgaattcc ctcatgagag agatcgaagg gactttgagc
      541 gcgatacagg ttgaaggact ggggcttgcg ttccgggagc cgctcctgtg ccgcatcgag
      601 ggcgaacttc tgtcgggcgg cctgacccga gagaccgtgt gtgtctgccg ggccatcccg
      661 ttcgtggtgc tcaaggcgct ggcgttcgac aagcgccggg aaaagaagga tgcctacgat
      721 ctagtctatg tcctccgaca tgcgacggcg ggcccgcgcg aacttgccgg acaaacgacc
      781 gccgaagaac gggcacaggc atcattctct cacgccatcg agaccatgcg gaatcacttc
      841 cagtcaccga cacaaaacgg cccggtccat tatgcgtcgt tcaccgaccg gagcagtgat
      901 gcaacggtag cgttcgccgc tgttcaagat tttcttggcg gattgtagga aaagaactcc
      961 ctaccgtccc ttcttcacga aacccttcag cgtttcatgc ttcttgatga gggcgaggag
     1021 cggctgatag ggcatctcca gcatgtcggc gatcatcttg accgaaaaac ggctgttgag
     1081 atatttacgg atggtgagga tctccttgtt gaagcgttcc tgcagggcgc ttacccgttt
     1141 tttcggcgcc ggtttccgcc ccggtttttt cgccttcttc gggcggccga ctcgtgcggt
     1201 tcgatcagtt ctccgggcgc acgacaaaga attcataggc atagcagtct gagtgccgtc
     1261 ggtgcatcgc gggctcctgc gcgagttgat cgaggacggc gatcgcgtcg gcgtcctcgg
     1321 catatttagc gcgcagttcc ccgatgcgaa gctccatcgg catgtagaaa tcatcccacc
     1381 atgcttcgtc aggcagggtg aagtgcccca tcagcgagaa cccggacttg gcgatggtgg
     1441 cgaggatgtc ggccactcga cccatagtcg ggtagtccca gtcgaaactg gctttcacct
     1501 cgggcggcgg gtttttcctg cgccagaccg cgtcggtgaa cgcgaggtag ccaccggctc
     1561 gcaacaaccc gtggcaaaca cgcagggcgt tctcaatgcc gatgtggtaa agcgcgccct
     1621 cggaccagac caaatcgaag ttcttgggcg gcagcccggg gttgcccatg tcaccgacca
     1681 tgggacgaat ccgcgcggaa agcccgcgtt cggcgaccgt cgcccgtaat cgatcaatgc
     1741 ttggcgcatg gttgtccaac gcgacgatgg ttccgggcgt cagttcggcg aggtgcagcg
     1801 tctggccgcc gacgccgcaa cctaggtcga gtatagcggg cgcggccggc aaatcgcgac
     1861 aaagccccaa cgctttttca gcgcaggcgc ggctacccgg cccctgtcta gggagcgcct
     1921 cgtaaagttc gaaaaaaatc gaccagaagc gcggcgatag atcgtgcata tcatttccct
     1981 ccttgggtta caggtcgtag cccgaggaaa acctcggagc cggtcacgat gtgatcgctg
     2041 tgttcaaagg gaaccacgag cggcttgaaa ccggcggcct cgatcagatc aagccacacg
     2101 gttcttgaga acagccccat caggtgacga tcgtgaacga catcgaagag accctgttcc
     2161 tctctcatga gataggccat gtcggtcaca tacctttcgg tcatcgcctc ggggacccac
     2221 cgccattcga tatagcgcag ggcgcgaccg ttgccgtcgc tacctcccga gccggtcccc
     2281 ggagtgaaat tctccgaaac aaaatcgggc tggaacatcg cgacacctcc gggagcggtg
     2341 tgcgcgaagg ccgtagcaat agcggcggca agatcgctgc gcgtggtcat gtaaacgacc
     2401 gcgtcatgca ccagaatgca gtcgaaaagc cgaccgagac ggaccgaacg catgtcgccc
     2461 tgaatatgtt cgcactccga attcagtcgg cgggacagat ccagcatggc gggagccaga
     2521 tcgacgagcg tacaggtcag cgtctccttc agataggcgg cattgtggcc ggcaccgctt
     2581 ccgagttcga gcatggtctg cggtgatcgc tgacagtggg cctcaaacaa acgccggtag
     2641 aaagccgcct cctccgcata gtcgcacacc ggcgtcagta gcggatacca ttctgacaga
     2701 tcacgataaa gtctgatatc ctgaccggga tcgttttcat cctctctgac catcgtcgtc
     2761 ccccgttgtt gcggtctgtc tgttcgccgt atccatcgta gggtcccccg cagggaaatc
     2821 aatttttcca ggggagggga gggacgggga gcgtccctgt ttttccagca ctccaacaca
     2881 aaattcccct tgcacctctc cccaacacac ggtatactaa agaaacgaag agtcttctag
     2941 gcaacgaaac cgttaacgga aggtgcgcat gaagtggcat tacagttgtc cactgtgtgg
     3001 agcatggatc tccgtcaaat gggcggagaa agattcccgt tttcaatgtc ccatctgcca
     3061 cagaccgcat tggcctcctt ctccttccgt tcaacgcgat gcctatgtca acgaccggac
     3121 gccgccggta gagatggcgg aagatgttta ccggctcaag ggccatgcgt gcacggtacc
     3181 ccgttgtcaa aacgacggcg acaccctcga tcatcgtatc ccgtgggaac attacaagaa
     3241 gggaacctgc gtggacaacc ttttccccat gtgctctgaa cacaattctt cgaaagggga
     3301 aaaggactat ttcgattggt tgcgggaact ggatcaacgc tgaggcatct ttccctcgcc
     3361 gtactcacat ccttcaaccg gacagcgtag atcgctccct tccggcggcc gaggatgctt
     3421 ctgaacgaaa agcgttcctc tgaactcatg ataatccaga cattcccgcc gataggcctc
     3481 ttccgggccg gcggcatact cgaaaaagaa aaggagccga gtctgatctt ccgtgtgttt
     3541 caggagttct tcccgcagat cggtatccga acgcccgacc cgacgaacga ccaattgctg
     3601 tcgttcatcc ttctcgcaaa gatggtaatt ccccgcggta gtacggttga ccatgagagt
     3661 aatgatgctc cgaaagagaa agcaggggcc gttcataaga aacgctccgt tgcaagtgga
     3721 ttgttccgtt agcggtcaac ataaaaatat gcccgttcga gaagatggtg tcaagaataa
     3781 cggaaaaaag ttcttacctt cccttcttca caaacgcttt gagcgtggga tgcttcttga
     3841 taagagagag gaaaggagcc cattattttg acgcggtggg tagttcgacg atcttctgtt
     3901 gaggatattt gatctttact tttttcatct ctccgttctg cacgatgaat atctcgtagg
     3961 tgccggccgg tacatccagc gcgatctgag gacccttccc tttcatgata ccgttcacga
     4021 atatcggcaa cccttccggc gcccgaatga tcaatttccg gctgagatgg gagagtcctt
     4081 cgagattgtt gcgcgccaga tcgacggaaa gagcgaagtt gatgctgttc gactcggtca
     4141 tttccgccgt aacgacaccg acgaccactc cgtccttgtc aaagacggga ccgccgctgt
     4201 tgccgcgatt gatcgggatc tgtgtctgga tgaccgcacg cccttccttg tcgatataga
     4261 cattcgatat catgcccgtg ttataggtcc agacggcgcc ccgtccgtga cctatcgccg
     4321 cggcccatga tccgaccttg atgtttgcat ccttcgagat atcgagaaag ggaagtccct
     4381 tcgcctctat cttgactaag gcgaggtcta tattgttttt tgcgcgctct attaccgttc
     4441 cctcgaatct cttgccgtcg ttcaggacga cattgacctt tttcccttcc ggcaccacat
     4501 gggcattggt caatatcaat ccatccgccg aaatgagaaa accgctcccc agcgtgttct
     4561 tggcgcccga tatgaagacg acgcagggag cgacgcgttc gaacaaagcc acctgacggg
     4621 tttctagatc gccgataggg tcggcggcga agatcgccac ggggaggaga gacagaaaaa
     4681 gaatgaaaaa gttcttcatg cgcctcgcct tttcctttag taactaacgc gcaggttgat
     4741 cccgccgccg gagggaagag cgaacggatc tatcatcaga tcaatccctt cggcgctcat
     4801 ctcttttgtt ttctcctcgg caccggaccc ttccgttctt tttttgagtt cgcggttata
     4861 tgcccgcgcc atgtcggcga cctcctttcc ggtcgccgga ttcttgttca gcagcacgcc
     4921 ggtgatccac gaggcggtcc cggttatcgt catcgccaga ccagaccaga aatagggtcc
     4981 gagcgggtcc tccgcctgct tttcccacgt caaggtcgtt tcattgaaac gataatagtc
     5041 cctcgaatcg ttcttccatg tctgcacgct tgccccggcg accacaaaca ccgcgccgac
     5101 gacggcgagc gagagccctc cgatgaacaa tccttttttc gccttttcac gacggtcgaa
     5161 ttcgatcgcc agatcctctc gtccgacctt tcggaagaaa acatcccagg tgagggcctt
     5221 tttgtatttc ccgatcctcg gttctctgtc ggtatcggga tccggccccg gagaaagacc
     5281 gtccttccat tcccaaaacc agagaagttc tctatcgtaa aaagtttccg gttctttttc
     5341 gtctttttta accgccgcac ttactgcatc cagaacgctt tgcgagacgc cgccttcgac
     5401 gatattcgtt tcatacggag acaacaattc gccccgttcc acggaaaagg cgttaagcac
     5461 ctcgccggtc ggccggtaaa gcgttacgac ggcgctcggc ttgaaatagc gggcgacaat
     5521 gatcagcgtg accgggaaac gggagcattt cttcacaacg gaagcatcat ccatcgccga
     5581 gatgtcgcct aacggagaat cgtccatgag catggcggcc ttccccgttc ctttaagagc
     5641 ctgcgccaac gctttgatca tagcgatcat cttcggattt cgttcacccg cgccaacaag
     5701 caacatcttt tctttgcccg agggaagata ggtcgccaca atctcggggg aaaaagacct
     5761 ctcccattct tcagaggcaa aaagagagac cgccgtcaga aagaacaaaa ggaaagaaag
     5821 ttgttttttc atttcaactc ctcttctctt tcagactttt tttcggtcgc caccggactt
     5881 cctttcgtga taaccgtggt cgagaacggt tcttgatcgc cgaaactaga aacataatta
     5941 caaacacgtg ccgtggaact acttgtctga taatagatac aacccttccc gcatccggcg
     6001 aaatgtggat cggtctctgt ataggctcca aaacaaatat attcgaagtc ccaatcatct
     6061 cgatcaaaat tattggtctc tagtcctctc gtttctcccg gcgcaagaga gaattgttcc
     6121 accggatcag gtttacagcc acaatcttca cacacaccat cacaggcata aagagcgatg
     6181 gtgatctcct ccgttgatgc atttttgaaa ccccaacgga ccggccagta ctcctcgcaa
     6241 gaaagcagag ggatcgtcag aagaatcatt gtaaggaatt tccgtttttt catccgaacc
     6301 tcccattgac accttttgtt cctagcaaaa tcaaagatat cgaaacagtg agacatgtca
     6361 aattcttcca ccctctttcc cggcgagaaa cgccaaagac tgtcaggcga cacatcgggg
     6421 tccaagaacc cgtggtaccg tgaaacagcg aagaccgttc tcctgcagta aaggccggat
     6481 cctcactttc ccttcttctt cacaaacccc ttgagcgtct catgcttctt gataaggccg
     6541 agtagcggct gatagggcat ctccagcatc tcagcgatgg tcttgaccga gaagcgatta
     6601 acgagatact tgcggatggt gagtatctga tcgttgaact gcttctggat gctgctcacc
     6661 ctcttcttcc gcgccggttt gcggcccggt ttcttcttcg ccttcgggcg accgaccttc
     6721 cgtctcggcg gtctgccaac cttcttcttg ggaggacgac cgaccttctt tttcgccgga
     6781 cggcctctct ttttcttggc gggacggcct cgtttcttct tggccggccg cccgggtttc
     6841 cggccggggc tcttcttctc gggggagagg agcagggcga agagcgagag gagagtggtc
     6901 ccttcctcgg agatgccttt caggacgatc ccctcggcga tgagatggac gacgaccttt
     6961 ttcttcagga gttcggcgag gagcgatatc cggtcctgtg tatcgagacc gagatactct
     7021 ttgccgggga agatgagcgt gtcgccgctt ttcaggttct cgaccgtcgt agagagcgct
     7081 gttcttttct tccacggctt cttcttggaa taggcgtctt cgactatctc cggcttgagt
     7141 tcgtgcgcgg tcatgtaggc ggccatcgcg gcacgctgct tctcgtcggg tctcccgacc
     7201 cggtttttgg tgacataggc gatgatcttt gacatggtac cctctatcgg ttgttgatgc
     7261 gttgaaggag gtctttcccg gctttgaaga acgggaagcg ccgttcgggg atcattatcc
     7321 gttcgccggt cttggggttg cggcttgtac aggcgcgaat gcgacgcacg gtgaaatggc
     7381 cgaagtcacg gatggcgatc cgttcctcgc gcatgagcgc gtttatcatg gcgtcgaaca
     7441 tcaggttgac gaactcttcc gcggccttct tagagatacc gcttcgggcg gcgagttctt
     7501 tcaccatgtc ggttttcttc attcctcgct cgatatcgct tctatggttt cagccaactt
     7561 tccgcgtcag gcctgacaat gatacgggca ttctgatacg ccatcctcat ggtaaggcaa
     7621 gttcgggcga catatcctcc ctcttcgcga tagaggacga gcgcgagatc ggccgggccg
     7681 tgtggatcgg tcagatagag ggggagcaag agttgaatgt gcccgttgaa atattgtgga
     7741 acggcggtcc gatagttgtt acgcacgagt ttcatggtgt attcgatggc acccttgagc
     7801 agatttctca aagcgtacct gttgccgcgc aacggttcgg ggaacctgtt cgtgttctcg
     7861 tcgatgatat gatcgtagtc gatggtgagc ggaagatgag gattgcagat caggagttcc
     7921 ggctgttgaa agtagtcggc ggggtaggga aggttccccg agaaataacg atttatcaag
     7981 acatcgcttt ccttctgggc gttcatgaag aaatagggag aagtggccgc aggatgtttg
     8041 ttcgtttgaa aaaacagaaa aatgtcctcg cggttcctcg tgagaagtcc ggtgttgaaa
     8101 cagacatgtt ctcgggaggc ggtgtaggcg atattctctt cttcagtcag tttttcgaag
     8161 gtgcgttcca ggtaattctg aagaatggag aaccgtttca ataaggtgtc tgaataatcc
     8221 cacggttccg gttccgcgag aatggcgatg tcttcaagcc gcttgtcgat ctcgggaaag
     8281 aacgcaaact cgagcaattt cgagcggggc gttttcatgt tttcctatcg gttattgatg
     8341 cgttgaagga ggtcgcggcc cgctttgaag tgagggagcc gtttagcggg gatctttatc
     8401 ggggaaccgt tccgcggatc cctgccttta tagccgccgt aatgccgtac gatgaagctg
     8461 ccgaagcccc gtatctcgat ccgttcctcg cgttggagcg ccgccgtcag ggcatcgaac
     8521 atgagggtca cgaaggtttc cgcttcccgg aaacggagtc cttccttctc ggcgatgatc
     8581 ttgatgagtt ctgatttcag catgagatat ctccacaggt tcaggatata ttctacgaat
     8641 tgcgggaagg agtcaagaat aaggagggga ctgtttcttt gacaaggcct tgcccggagg
     8701 tacactgttt cgagtataac ggagtagggg gagaaccatg tctctgacga agatcgagtt
     8761 agcgaacctt ctctatgagt cgatgcatgt cgacaaaaaa caggccgaag aactggtcga
     8821 tctgttcttc gaagaattga agaaaggcct cctgaaggac ggagaactca agctctcggg
     8881 gttcggtaat ttccagatca aacagaaaac taagcgacgc ggtcgcaatc cgaagaccgg
     8941 taaagacctg accatcgatg cccggaaggt cgtgatcttc aagcgtagcg tggtgttgaa
     9001 gggaagattg aatagcgcaa aggggtaggg tcattgtcgt ttttcgtcag aaaccatgct
     9061 ctgttttcct gataaaagat cttcttgaca tacataagat tctgcataca atcgtaagta
     9121 cgataatgga gtagggggac ctactatggg tagaaggatc cggacaacgc tttcgctcga
     9181 tgccgggatg gtgcgggaac tcgacgatgt ctccgtcgag ttgaaacaga agaaaagtca
     9241 cctcatcgaa caggcatggc agttctatct tgactatctc gatgttaaga tcgccgaaaa
     9301 acggctggcc gcggtcaaga acggtaaggt ccgcacgata ccggcgaaga aggcctttaa
     9361 ggaactgggc ctcgattgat ggtgtatgct atcgagttcg agccgcaggc gctcaaggaa
     9421 ttgggcgcca tagatcggcc ggcacaacgc ctcatccggg agaagatcct gctgctcggc
     9481 gagaactacg ccgcgctcaa gaataacatt acgcaactca agggacagaa ggaaccgttg
     9541 ttccggctcc gggtcgccga ttaccgggtg atcttttcca aggacgatac ccgcatcatc
     9601 atcttcatcg ttcgcgtgcg tcatcgccgc gaggtgtacg agtaacgcaa cggcctcccg
     9661 ggttgcctca ggacgagaga acggatggcg gtcaagggga cgatattcgg ggatcgaggt
     9721 cgtgcccgca gggagtatag tataaatact atactcctaa taatatcaaa tacttacttc
     9781 gcataaccta ctttatgtca acttgagagc ggcttgtggg tggaagttct tgaaagaact
     9841 ttactttttc ggcagacttt cgaggagtcc cgctatctgc gacgcggcct gcgggggcat
     9901 ctgctgtttc tgcatttcgc gcaggctgtt gccgatgata tcttcgttcc ccgcgaggtt
     9961 cttgcggaac agcgccgcca gatagagata ctggtcagga tttagggatc cgtccttgat
    10021 gttctcgtgg atcttctcga aatgttcctt cttgaggctg tagacactga gggtgttgac
    10081 ggcgttcatc ttcacgaggt tcgatttgtc accgagcata tcgagaagca tctgttccga
    10141 ttcgggggtt tgggtgaagc ggaccgcgtt ggccaccgag gcgcggaccc ggtcgttgag
    10201 tgaatctttg tattctgcgg ccacggcgat gttcgacgag ttggcgacat tgccgatggc
    10261 gtccagcatc cgttccgccg attcgggggt cgccgacttc cgcagttcct cgaccaattt
    10321 gttgttaagc atcgcccctc ggataccgtc gccgccccgc gtcgtcttcc ccgccacggc
    10381 gcccagaata agcgacgcgg tggaacgcaa cttattggtc gccagagcgt cgtcgtacaa
    10441 cttctcggcg aacgagacgg tctgcgggtc gggcgtatca agataactga tattctgaag
    10501 caacagcggg taaaggcgat cattcttggc cgtatcgcca gccagcaact gccgcatcac
    10561 cgtctgtgct tcgggatgtc ccaccgaggc aagagccccc atgatgagca tccgcccgtc
    10621 gctggagaag gtcgggtcgt tgaacagcgg agccagatcg gtcgtccgtt cggggtaccg
    10681 tttcaggtag ccggccatcc gccacatgaa gagtttcttg tcccctatct cgccgctcgt
    10741 accataggcg cggagggtgt cgagcatttc ggtgacggtc aggttgcgcg cctgttgttc
    10801 gtacattttc ttcttgatct cgtcgttcaa ttcaaccgtt ccgagacgac tggaacggag
    10861 ttcattgagc cgcgccagcg ccccgtcgag atcgaatccg cggcggcgta catcgaccaa
    10921 ggtcatcttg aggttgacct tcagcaaaaa gatatctttc ccctctcggg cgcaggtttg
    10981 atcctgcgtt ccttttattt ccgatatcaa cgcatccttc acttgatacc ggaacgatcc
    11041 ggtcccgcgc tgttcaagcg ccttggtctg cttagggaag gccaaaagtt cttcgtagcg
    11101 ctcgatgttc ttggtgacgg tcgtgtcccc taccccgatg ccgtcgtacg aataactcac
    11161 cttgtatcga ccgttctgat cccactcctc ggctggcggt ttcttcttgc cggaaagtgc
    11221 tgtttggaaa taggccatta tgagtttcat cgtgccgacg aagacgggat cttcgttctt
    11281 gcggaaccgg acatcgctca ccgcgccgaa accgtcgtga accacacccg catgacggcc
    11341 catgaatatc tgttcacgcg cctcggggtt ttcgaggata tcctgtccgt tgaaatagaa
    11401 ggaacaagca ccaaccttca cgacggccag ttccgaaaag aaagaaccat cagggagtct
    11461 tttcaccggg gtcagtgaaa accgcagggt acagtcgaac ttgcccgaga ggtctttcgt
    11521 tcccccttcc gggttgagat gggaattgac cgtttcgccg gcatagacga actcgtactc
    11581 gtaggtggta ccgatcttcc agcggagcgg gttccggtcg ccgaagaagt gcgtgtaggt
    11641 gaaataggaa atgccagcaa gaagcaaaac agctacgcta atagtaaata ttctatttct
    11701 tgagaatcgt ggcttgaggg gaacttcttt tctaggtttc atgaaatcct actctcggag
    11761 ttattggcac ttttgtgggt cttggtactg gatttcttct tcaagggtag tcgcagattc
    11821 gggatatttg gtattccacc aattgacata tttttggact tcgctttcgg gcaaacaatt
    11881 tccaccaata ccaatagctt ctaatttaga cagtgtcgtt ataaacgata tatttgtgag
    11941 gtaattactg cctgctgcaa tatctgtcaa ctccggcagg ccgctgagtt cgttcaaact
    12001 ggtcaagccg cagttggaga cattgagggt ctgcaacttc ggagcggtga attgcgcgcc
    12061 gagggtcgtg agggtgggag tattaattac tctaacactt tctaggttag gcaacgaaga
    12121 gagtgcggct agatttgtca atttgttctc tcttaaatcg atattcttta cattcaatat
    12181 ctccgacaat gcggaaatat cttccagttg agattgttga tattctacgt agagtagccc
    12241 tcccaactct tttatatcgt aaaggttctt aacccccccc cccc
//
LOCUS       DAOQUE010000016        12039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_5973_length_12039_cov_3.720377, whole genome shotgun sequence.
ACCESSION   DAOQUE010000016 DAOQUE010000000
VERSION     DAOQUE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12039
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5973_length_12039_cov_3.720377"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PKH10_01050"
     CDS             complement(<1..431)
                     /locus_tag="PKH10_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26740.1"
                     /translation="MKIVRLFIVSALSITLLFACSEKKSDTFTPRTAKKTVTAATGGT
                     VSTTDGSASVEIPAGALAQDTEITITEKKKDDYPEEEKLASSVWHFGPDGLTFSKPIT
                     MSIKLDRNKVEDEVGVIAVHREGAWHSKGYAGGEYAAGDPI"
     gene            597..2144
                     /locus_tag="PKH10_01055"
     CDS             597..2144
                     /locus_tag="PKH10_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26741.1"
                     /translation="MTRAAEALAKFKTIEVGYDLTKLTDDIRDALPHLIAALDVVTDI
                     YLRQQDERLPVLWKKVMAGSDEEKKRFYHRFKGPWDQLSDYATVYPDDLPDRAKGCAF
                     YPDGMDAAALEAAFAKLPAAEQEKLRDTYTVVRKEGDKLTAIPYHEYHRADLERLAGH
                     LKKAAALIKHEGLKKYILNRAEGLLTGKYPETEAEWVKLTDAPLELVFGPFEVYADGV
                     LGLKSTYEGFLLAVDHEKGARLKEIEMNLDKLNAAFPMPAGAKPAVGGMAPMVVAHEL
                     YASGEAYHGIMTSAFNLPNDPWVRGNVGWKQVMIYNIMQAKFKTCTSVIAKRISEGRI
                     TADFDPYFFFVLMHEVSHGLGPAYRADGTKVAAAIGSAYTTVEEAKADTGGLVLLLKM
                     GGKYGVPAFGTRQMLDSFFAGLFRSMRFGVHEAHGGANVIEYNWMKDRGVIEVKPDGS
                     FETHDADFAKAADDLLEELCRIEAAATPDEAAAFVKKWAHPGKDILDALEKLKDIPID
                     ITPIYTR"
     gene            complement(2157..3461)
                     /locus_tag="PKH10_01060"
     CDS             complement(2157..3461)
                     /locus_tag="PKH10_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26742.1"
                     /translation="MKHRTLLWVVFCLLFLAAACDDGNDTVTDTDSAVTDELVTDADI
                     VPSSITDQNFGAFAAWSSPIEDFRSFGVDNMIEGFSQEVTDLAVLDGRLWFGYGDANL
                     NFGTKMLKYADGGIQFRYFDDPDNSTAKGDLVSGDEMIEHFRQFDGGLWYAGVDSTDP
                     DELTSRPLIGGNFYRHDEDGWKKFRSVPGGEHVHDTALFDGRIFAVGSGATDRTEWEA
                     GNIFRYLWESSDRGETFTVTHRIGHPTQGGAGDSRFVHLLPVGDTMYVFGYWSDFAAN
                     QAYVANAYYDGETVTEFVAEDTSILKGIWPTATLSLPNDTGLVWGVNVDTATLKYQIW
                     QVAADGTVTALTAFAGKQVFDVGYYPETEELIYLTYDGDAYAELVDPVATTIYAAPLS
                     DPNDIKEIATYNDTPRIVSLAYWQEHLFAGTNDCRIFRANAE"
     gene            complement(3492..5030)
                     /locus_tag="PKH10_01065"
     CDS             complement(3492..5030)
                     /locus_tag="PKH10_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26743.1"
                     /translation="MKQSVFCLCCLCLIGLIAACDSSTANKGTDTVGASDSDTVATDE
                     LAGDTTITDGPAQDTDAPLPDLPTDDDTPVTDELVTDTDAPGNDELLTDTDGYINPAC
                     SSVGETCVTGIDCCSGACEKNPIGDFVCIDAELCEPVGGDCTVAADCCSLGCDGTTCI
                     DSLCLVTNDPCIDDAECCSNRCEVNFCASGGACLPAGEECSSGGTCCSMNCAEASDGK
                     MRCMGLGVCRSEGEVCSEDADCCNYQCTGEGFCLVQQECDVAGEACNDPKACCSGVCA
                     DDGSGYKFCQSLGGCKVFGELCHADAECCNNPANGGPGVCTEVADGIGRCENPTGCAP
                     AGELCGPGYHDCCPCATPQDSPGCPNDNGDLFCQETIFGISRCFSANCVIEGGPCDSD
                     DDCCGGNCNEENTCGPGIECLPNLSPCAMPDQCCCNICSPDGNGDLVCCPGDIECIPE
                     GEHCTTDLDCCSGNCSVNGICGPDISDCIPVGGECEKDEDCCSGSCYEVTGTCNSPFF
                     FFVY"
     gene            5189..6202
                     /locus_tag="PKH10_01070"
     CDS             5189..6202
                     /locus_tag="PKH10_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNT26744.1"
                     /translation="MAKIAILHTGGTFAMESNEQGKLRPGTYAFDYIERSVYPLFEDH
                     IEQFQLLNIDSSQFRPAHWKLIAERIAAMYENYDGFVIVHGTDTMAYTAAALSFFFEN
                     LDKPVVLTGSQIPLKDKRSDALQNLVAAIEVALRGNLNEVVVVFHTAAFRGNRVKKRD
                     AWDFDAFYSPNHPKLIKLGIGLERLDRLFLPRPSGIFSVNTSLEERVMLVPFFPGFDF
                     SMLLPIVRDEKVKGIVLEAYGSGNIPSDNPGLEEIFKEASANAFPIVVCSQSPAGKVN
                     LHLYEAATKAEFYGLISAADMTREATLVKLMVALGRFGSDLKKIKKFMMRSIAGEKVT
                     EPC"
     gene            6196..7131
                     /gene="era"
                     /locus_tag="PKH10_01075"
     CDS             6196..7131
                     /gene="era"
                     /locus_tag="PKH10_01075"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNT26745.1"
                     /translation="MLKEQLAHSGFVAVIGAPNTGKSTFVNTAIGKKVSIVSPKVQTT
                     RNAIRGIYTTDDAQVIFTDTPGITPDDLGKLLNDWMNRTAFSKAREADAVLLFVDVTT
                     QHPEQGPGKEEIDILCKLRVQCPVLLVANKVDAVKKMRVDDTVAVLGKQYPFAGAHRI
                     SALSGAGVPELLAAVTALLPAGPQYFEAAEPSDQTDEFLVAEIVREKMFHLLQRELPY
                     HSAVIVERMYDHETIAGMLVINATINVARKSQKAIVIGEKGRMLKRIGTQARVELETL
                     FGRKIHLLLHVRVEEDWFAKERSLKKMGFEKEFDE"
     gene            7128..7733
                     /locus_tag="PKH10_01080"
     CDS             7128..7733
                     /locus_tag="PKH10_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26746.1"
                     /translation="MRTMVLLFVLCAVLLPLSGQTQKGPGEEEIRFYESIIATRDEGL
                     AVCRTLAQTVKANIELYASIDKKSAHPDDRAVTRAGGELVMEAFTLAKGVCDAFAILD
                     DDRAADIAKKDFPRFKKEKMPTDTRSFRINVIKLGLMEVHDAVGWWNRNMVEMNEGMK
                     KKPQALVIKELLTATLRDAPEMRKRLDAIKKKYPVLTEERP"
     gene            7730..9085
                     /gene="der"
                     /locus_tag="PKH10_01085"
     CDS             7730..9085
                     /gene="der"
                     /locus_tag="PKH10_01085"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNT26747.1"
                     /translation="MTPRPINNIKPLIAIVGRPNVGKSTLFNQILGKNAAIVDDLPGV
                     TRDLHYEDILYDDKPFTVIDTGGFHIDEAGDAITLGIRRQIEEVFASVDGVLVVGDGL
                     AGLHPADRDILDIVRRTGIRHWLVVSKVDDEHREPHAAEFYETGADQVFPVSGTTGYG
                     VRDLLDEMTREFWTAREIAEKLPSPTDEVRIAVIGRPNVGKSTLVNALLGEERMLVSP
                     IPGTTMDPIDSVFTRDGRTYRIIDTAGIRRKKNISLKMENAAVVRAFKSIDRADIALF
                     LMDATEPATDQDLRVIGLAHEKNRGIILALNKWDLVVKDEKTYDKLVKDLRERIKFAP
                     YAPILSLSALERQRVGKVLDLVDEVHERYTRSIDTATLTDWLMRTVQHTPPPLAKFHK
                     PVVFRKAEMSSVRPPVVMISVNKPDSVHFSYERYLINRFYASFDYTGVAVRFLFRRSS
                     R"
     gene            9088..10023
                     /locus_tag="PKH10_01090"
     CDS             9088..10023
                     /locus_tag="PKH10_01090"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNT26748.1"
                     /translation="MKKIVERVVFLAGRFLSYVDARRPLRVIWNTLKGFVDDDCLDRA
                     AAVSFYALLSFIPFLIIVGAMLGLFIDHLSTPDTTPQQLIATVRGYLKIIIPYLQDEQ
                     VTGFLSLSRHTASLSTIGFMSLAVSASLLFSTLSDTLNRIFGTKPIHFVFSRLVGFLI
                     IVALTLLLFFLHYFSAMVLSVVTLLRDQFPAVQPVIDFLTGNGLLWSIPIITLFILLF
                     FSILIYAFTMAARIHFVAVIGGALLFSGLWNGAKYLFNVYITKLSSYSIVYGSATWFA
                     AAILWVYYSILLLLLSMEFIRALNGEIGRKKGDAA"
     gene            complement(10033..>12039)
                     /gene="dnaE"
                     /locus_tag="PKH10_01095"
     CDS             complement(10033..>12039)
                     /gene="dnaE"
                     /locus_tag="PKH10_01095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNT26749.1"
                     /translation="VVISDKPLWEYSPIFKDKDGALVTQYDKDMVEKAGLVKFDFLGL
                     KTLTMIQHAVDLIRRNQPGFDIARIPLDDAATYRTLWTKTTKGVFQMESVGFGKMIKQ
                     MRPDRIEDLIAAVALFRPGPMAIIPNYVRRKHGREEVVYDHPLVEEILKETYGLIVYQ
                     EQVMQISVAMAGFSMGQADTLRKAMGKKQADAMKKAEFDFFEGSRKKGIDLAVARGVF
                     DLMKKFAEYGFNKSHAAVYAFIAYRTAYLKTHFPSEFFAALISSDAGNSAKIFEYIAD
                     ARAMDIAILPPDINRSELSFSIEQGTIRFGLGAVKNVGEGAVEEILKEREKNGPFASL
                     CDFAARINQTRVNSRAIEFLIKAGAFDLTGVKRGDLFSILPYALREGEKRYQDKVCGQ
                     TSLFSLMSPDTAAGSAPEGNDDVSLLRSVDRATPAWDIFHTLALEREVLGTYVSNHPA
                     AYFARESRNLGFEDLEEYLSAIEESGRAPREKVWVLGTVTTDIKPQKGRDGDYYLRGV
                     IEGVGAAVEFVINKIPDASSPLVDKLRHPLPLAFRISPRIVRNMEEERIDRVEAVIED
                     IAKDVLTVTEYLTTGSEGRVRAVLEAPREVIDASLDLIKRLRERSAIEESEMPLAVKI
                     LFPEIKWAVYLEGMVWLSEDRVRMLKDRFGPDRFYFLRRQAPAQ"
BASE COUNT         2351 a         3718 c         3677 g         2293 t
ORIGIN      
        1 atgatggggt cgcccgccgc gtactcgccc cccgcgtagc cctttgaatg ccacgcccct
       61 tcgcggtgga ccgcgatgac gccgacctcg tcctccacct tgttgcggtc gagttttatg
      121 ctcatggtga tcggtttcga gaaggtgagt ccgtcggggc cgaaatgcca gaccgaactg
      181 gccagtttct cctcctcggg atagtcgtcc ttcttcttct cggttatcgt tatctcggtg
      241 tcctgcgcaa gagcgcctgc cggtatctcg accgatgcgg agccatcggt cgtcgaaacg
      301 gtgccgcccg tagccgccgt gacggtcttt ttagcggtcc tcggcgtaaa ggtgtcgctt
      361 ttcttctcgc tacaggcgaa aaggagcgtg atgcttaacg ccgaaacgat gaacaggcga
      421 acgatcttca tggagccctc ccttttttga agacaacaac cgacgaagga tatcatatcg
      481 gcataaaaac gcaagaagaa tggggggaga gaggtgcttc ggttggtttt tcttttgact
      541 tcaccgccgc cgcgcgctat ccttcgtcga ccgaaaacca tttcagggag gttcccatga
      601 cccgcgccgc cgaggccctt gcgaagttca agaccatcga ggtcggctac gacctgacca
      661 aactgaccga cgacatccgc gatgccctgc cgcacctcat cgcggcgctc gatgtggtga
      721 ccgacatcta cctgcgccag caggatgaac ggcttcccgt cctctggaaa aaggtgatgg
      781 ccgggtccga cgaggagaaa aaacggttct accaccgctt caaggggccg tgggaccaac
      841 tcagcgacta cgccaccgtc taccccgacg acctccccga ccgcgccaag ggctgcgcct
      901 tctatcccga cgggatggat gccgccgcgc tcgaggcggc cttcgcgaaa cttcccgccg
      961 ccgaacagga gaaactgcgc gacacctaca ccgtcgtccg caaggagggc gacaaactca
     1021 cggcgatccc ctaccatgaa tatcaccgcg ccgacctcga acggctcgcc ggacacctga
     1081 agaaggcggc cgctctgatc aaacacgaag ggctcaagaa gtacatactc aaccgcgccg
     1141 aggggctcct caccggcaaa taccccgaga ccgaggccga atgggtgaaa ctcaccgacg
     1201 cgccgctgga actcgtcttc ggccccttcg aggtctacgc cgacggcgtg ctgggcctca
     1261 aatcgaccta cgaaggcttc ctgttggcgg tcgaccatga aaagggtgcg cgactgaaag
     1321 agatcgagat gaacctcgac aaactcaacg ccgcattccc gatgccggcc ggcgcgaaac
     1381 cggcggtcgg cggaatggcg ccgatggtgg tggcgcacga actctacgca tcgggcgagg
     1441 cctaccacgg catcatgacc agcgccttca acctgccgaa cgatccgtgg gtccgcggca
     1501 atgtcggctg gaaacaggtg atgatctaca atatcatgca ggcgaagttc aagacctgca
     1561 cctcggtcat cgccaaacgg atatcggagg gtcgcatcac cgccgacttc gatccctact
     1621 tcttcttcgt cctcatgcac gaggtgagcc acggactcgg ccccgcctac cgcgccgacg
     1681 gcacgaaggt ggcggcggcc atcggttcgg cctataccac cgtcgaagag gcgaaggccg
     1741 acacgggcgg gctcgtcctg ctcctcaaaa tgggcggcaa gtacggcgtc ccggcgttcg
     1801 gcacgcgaca gatgctcgat tcgttcttcg cgggactttt ccgctcgatg cggttcggcg
     1861 tccatgaggc gcacggcggc gcgaatgtca tcgaatacaa ctggatgaag gatcgcggcg
     1921 tcatcgaggt gaagcccgac ggttcgttcg agacgcacga cgccgacttc gcgaaggcgg
     1981 ccgacgacct gctggaggag ttgtgccgca tcgaggcggc ggcgacgccc gatgaggcgg
     2041 cggccttcgt gaagaagtgg gcccatcccg gcaaggacat cctcgacgcc ctcgagaaac
     2101 tcaaggacat ccccatcgac atcacgccga tctatacgag ataagaacta aaaaggttat
     2161 tcggcgttcg cccggaatat gcggcaatcg ttggtgccgg cgaagaggtg ttcctgccag
     2221 taggcaagcg aaacgatacg cggcgtatcg ttataagtcg ctatctcttt gatatcgttc
     2281 gggtctgaca gcggcgcggc gtagatggtc gtcgccaccg ggtcgaccag ttccgcgtaa
     2341 gcgtcgccgt cgtaggtaag atagatcagt tcttcggtct cgggataata gcccacatcg
     2401 aacacctgct tcccggcgaa ggcggtaagc gccgtcaccg tcccgtcggc cgccacctgc
     2461 cagatctggt atttgagcgt cgcggtatcg acattgacac cccagacaag gccggtgtcg
     2521 ttgggaagcg acagggtcgc ggtcggccag atgcctttga gtatgctcgt gtcctcggcg
     2581 acaaattcgg tcaccgtttc gccgtcgtaa taggcgttgg cgacataggc ctgattcgcc
     2641 gcgaagtcgc tccagtagcc gaagacatac atcgtatcgc ccaccggcag gaggtgcacg
     2701 aaacgcgaat cgcccgcgcc gccctgcgtc ggatggccga tgcggtgggt cacggtgaag
     2761 gtctcgccgc ggtcggacga ttcccagaga tagcggaaga tgttgcccgc ctcccattcg
     2821 gtacggtcgg tcgcccccga accgacggcg aagatgcggc cgtcgaaaag ggcggtgtcg
     2881 tggacatgtt ccccgccggg aacggagcgg aacttcttcc agccgtcctc gtcgtggcgg
     2941 tagaagttgc cgccgatgag cgggcgcgag gtcagttcat cgggatcggt cgagtccacc
     3001 ccggcgtacc agagcccgcc gtcgaactgg cggaagtgct ctatcatctc gtcgccgctg
     3061 accagatcgc cttttgccgt gctgttgtcc gggtcatcga aatagcggaa ctggatgccg
     3121 ccgtcggcat actttagcat cttcgtgccg aaattgaggt tggcgtcgcc gtagccgaac
     3181 cagagccggc cgtcgagaac ggcgagatcg gtcacctcct gcgagaagcc ttctatcata
     3241 ttgtcgaccc cgaaggaacg aaagtcctcg atgggggagg accatgccgc gaacgccccg
     3301 aagttctggt ccgtgataga ggaggggacg atgtcggcat cggtcaccag ttcgtcggtg
     3361 acggcgctgt cggtgtcggt cacggtgtca tttccatcgt cgcacgcggc ggcgaggaac
     3421 agcaggcaga aaactaccca cagtaaagtg cgatgtttca tgtgagaccc cggtatcagt
     3481 tcattctcat gtcaatagac gaaaaagaag aaggggctat tgcaggtgcc ggtcacctcg
     3541 tagcaggagc cggaacagca atcttcatcc ttctcgcact cgccgccgac cgggatgcag
     3601 tcggatatgt cgggtccgca gatgccgttg acgctgcagt tgccagaaca gcagtcgagg
     3661 tcggtcgtgc agtgttcgcc ctcggggatg cactcgatat cgccggggca gcagaccaga
     3721 tccccgttcc cgtccggtga gcagatattg cagcagcact ggtcgggcat ggcgcagggg
     3781 gagaggttcg gaaggcactc gatcccgggg ccgcaggtgt tctcttcgtt gcagttgccg
     3841 ccgcagcaat cgtcgtcaga atcgcagggg ccgccctcga tgacgcagtt ggccgagaaa
     3901 cagcgcgata tgccgaatat cgtttcctga cagaacaggt cgccgttgtc gttagggcag
     3961 cccggcgaat cctgcggcgt cgcgcaggga caacagtcgt gatagcccgg gccgcacagt
     4021 tcgcccgccg gcgcgcagcc ggtcggattc tcgcagcgcc cgatgccgtc agccacctcg
     4081 gtgcagacgc cgggaccgcc gttcgccggg ttgttgcagc actcggcatc ggcgtggcac
     4141 agttcgccga acaccttgca tccgccgagc gactggcaga atttatagcc gctgccgtcg
     4201 tcggcgcaca cgccgctgca acaggccttc gggtcgttgc aggcctcgcc ggcgacatcg
     4261 cattcctgtt gtaccagaca gaagccctcc ccggtgcact ggtagttgca gcagtcggcg
     4321 tcctcgctgc agacctcgcc ctcggaccgg cagaccccga gtcccatgca gcgcatcttg
     4381 ccgtcggagg cctcggcgca gttcatcgaa cagcaggtcc cgcccgacga acactcctca
     4441 ccggcgggaa gacaggcgcc gccgctcgcg cagaaattga cctcacagcg gttggaacag
     4501 cattcggcgt cgtcgatgca cgggtcgttg gtgaccaggc agagagaatc gatgcatgtc
     4561 gtgccgtcgc agccgagcga acagcagtcg gccgccaccg tgcaatcgcc ccccaccggt
     4621 tcgcaaagtt ccgcatcgat gcagacgaag tcgccgatcg ggttcttctc gcaggcgccg
     4681 gagcaacagt cgatgccggt gacgcaggtc tcgccgacgg aagagcaggc ggggttgata
     4741 tacccgtcgg tatcggtcag cagttcgtcg ttcccggggg catcggtatc ggtcacgagt
     4801 tcgtcggtca ccggggtatc gtcgtcggtc ggcaggtcgg gaaggggggc atcggtgtcc
     4861 tgcgccggtc catcggtgat ggtggtgtcg ccggcgagtt cgtcggtcgc gacggtgtcg
     4921 gaatccgatg cgccaacggt gtcggtgccc ttgttggcgg tcgaagaatc gcaggctgcg
     4981 atcaggccta tcagacacag acaacacagg cagaacacgc tttgtttcat gggacgcctc
     5041 ccgaacggtt gttccttaac attatcgagc atacccgaaa agaaaccttg ccgcaacttt
     5101 tttcgccccg cctctgcgaa cataaacttg ccttttcgag aggttagggt tagaaaacga
     5161 cccgaccaac agcgttatgg agcgtccgat ggccaagata gcgatactcc acaccggcgg
     5221 caccttcgcc atggagtcga atgagcaggg gaaattgcgc cccggcactt acgccttcga
     5281 ctacatcgag cggtcggtct atccgctctt cgaggaccat atcgaacagt tccaactgct
     5341 caacatcgat tcgtcgcagt tccgccccgc ccactggaaa ctcatcgccg agaggatcgc
     5401 cgcgatgtac gagaactatg acgggttcgt catcgtccac ggcaccgaca cgatggccta
     5461 caccgccgcc gccctctcgt tcttcttcga gaatctcgac aagccggtgg tgctgaccgg
     5521 ttcacagata ccgctcaagg acaagcgctc cgacgcgctc cagaacctcg tcgccgcgat
     5581 agaggtcgcc ctgcgcggca acctgaacga ggtggtcgtc gtcttccata ccgccgcctt
     5641 ccgcggcaac cgcgtcaaaa agcgtgacgc gtgggatttc gacgccttct acagcccgaa
     5701 ccacccgaaa ctgataaagc tcggcatcgg actggaacgg ctcgaccggc tgttcctccc
     5761 gcggccgagc ggtatcttct cggtcaacac ctcgctcgag gagcgggtga tgctcgtgcc
     5821 gttcttcccc ggcttcgatt tctcgatgct cctgcctatc gtccgcgatg aaaaggtgaa
     5881 gggcatcgtg ctcgaggcct acgggtcggg caacatacct tccgacaatc cggggcttga
     5941 ggagatattc aaggaggcat cggcgaacgc gttccccatc gtcgtgtgca gtcagtcgcc
     6001 cgccggcaag gtgaatctgc acctctacga ggcggcgacc aaggcggagt tctacgggct
     6061 catatcggcc gccgacatga cccgcgaggc gacgctcgtc aaactcatgg tggcgctcgg
     6121 ccgattcggg agcgatctca agaagataaa gaagttcatg atgcgctcca tcgcgggcga
     6181 aaaggtgacc gagccgtgct gaaagaacaa ctcgcccaca gcgggttcgt cgccgtcatc
     6241 ggagcgccga acaccggcaa atcgaccttc gtcaacactg cgatcggcaa gaaggtgagc
     6301 atcgtgtcgc ccaaggtgca gacgacccgc aacgcgatac gcggcatcta cacgaccgac
     6361 gacgcgcagg tgatattcac ggacaccccc ggcatcaccc ccgatgacct cggaaaactg
     6421 ctcaacgact ggatgaacag gaccgctttt tcgaaggcgc gcgaggccga tgcggtgctc
     6481 ctgttcgtcg atgtgactac ccagcacccc gaacaggggc cggggaaaga ggagatagac
     6541 atcctctgca aactgagggt gcagtgcccg gtcctgctcg tcgccaacaa ggtcgatgcg
     6601 gtcaagaaga tgcgggtcga cgacaccgtc gcggtactcg ggaaacagta ccccttcgcc
     6661 ggagcgcatc gcatcagcgc gctttcgggc gccggggtcc ccgaactgct cgcggcggtc
     6721 accgcgctcc tgcccgccgg gccccagtat ttcgaggcgg cggagccgtc cgaccagacg
     6781 gacgagttcc tcgtcgccga gatcgtacgc gagaagatgt tccacctcct gcaacgcgaa
     6841 ctgccctatc attccgcggt tatcgttgag cggatgtacg accacgagac catcgccggc
     6901 atgctcgtca tcaacgccac catcaatgtg gcgcgcaaga gccagaaggc gatcgtcatc
     6961 ggcgagaagg ggcgtatgct caaacggatc ggaacgcagg cgcgggtcga actcgaaacg
     7021 ctgttcggca gaaagataca tcttctgctt catgtgcggg tcgaggagga ttggttcgcg
     7081 aaggaacgct cgttgaaaaa aatgggattc gagaaggagt tcgacgaatg agaacgatgg
     7141 tcctgctgtt cgtattatgc gccgttctgc taccgctctc cggtcagaca cagaaggggc
     7201 cgggcgagga ggagatacga ttctacgagt cgatcatcgc cacccgtgat gaagggctcg
     7261 cggtctgccg gacgctcgcg cagaccgtta aggcgaacat agaactgtat gcatcgatcg
     7321 acaagaaaag cgcccacccc gacgaccgcg ccgtcacgcg ggccggcggt gaactcgtca
     7381 tggaggcttt cacgctcgcc aagggggtct gcgacgcctt cgcgatcctc gacgacgacc
     7441 gcgcggccga catagcgaaa aaggacttcc cgcgcttcaa gaaggaaaag atgccgaccg
     7501 acacccgttc cttccgcatc aatgtcatca aactgggact catggaggtg cacgatgcgg
     7561 tcggctggtg gaatcggaac atggtcgaga tgaacgaggg gatgaaaaaa aagccgcagg
     7621 ctctcgtcat caaggaactg ctgacggcga ccctgcgaga tgcgccggag atgcgcaaac
     7681 ggctcgacgc gataaagaag aaataccccg tgctgacgga agagcggcca tgaccccgcg
     7741 cccgatcaac aacatcaagc cgctcatcgc tatcgtcggg cggcccaatg tcgggaaatc
     7801 gacgctgttc aatcagatac tcggcaagaa tgctgccatc gtggacgacc tgccgggggt
     7861 gacgcgcgac cttcattacg aggatatact gtacgacgac aaaccgttca cggtcatcga
     7921 cactgggggc tttcacatag acgaggcggg ggacgccata acgctgggca tccgccgtca
     7981 gatcgaggag gtgttcgcgt cggtcgacgg tgtgctcgtc gtgggagacg gtctggcggg
     8041 gcttcacccg gctgaccgcg atatactcga catcgtccgg cggaccggca tccgccattg
     8101 gctcgtcgtc tccaaggtgg acgacgagca tcgcgagccg cacgccgccg agttctacga
     8161 gacgggggcc gatcaggtgt tcccggtctc cggcaccacc ggctacggtg tgcgcgacct
     8221 gcttgacgag atgacgagag agttctggac cgcgagggag atcgcggaga aactgccgtc
     8281 gccgaccgat gaggtgcgta tcgcggtcat cgggcgaccc aatgtgggga aatcgacgct
     8341 ggtgaatgcg cttctgggcg aggaacggat gctcgtctct cccataccgg gcaccacgat
     8401 ggaccccata gattcggtgt tcacccgcga cggtcgcacc taccggatca tcgacaccgc
     8461 cggcatccgg cgcaagaaga acatatcgct gaagatggag aacgcggcgg tcgtgcgggc
     8521 attcaagtcg atagaccgtg ccgacatcgc gctgttcctg atggatgcga ccgagccggc
     8581 gaccgatcag gaccttcgcg tcatcggact cgcccatgaa aagaatcgcg gcatcatcct
     8641 cgccctcaat aagtgggatc tcgtcgtgaa ggatgagaag acctacgaca aactcgtaaa
     8701 ggacctgcgc gagcgcataa agttcgctcc gtatgccccg atactctcgc tgtcggcgct
     8761 cgagcgccaa cgggtcggga aggtgctcga tctggtggac gaggtgcatg agcgctatac
     8821 gcggtcgatc gatacggcaa cgctcaccga ctggctgatg cggacggtcc agcacacccc
     8881 gccgccgctc gcgaaattcc ataagccggt cgtcttccgc aaggccgaga tgtcgtcggt
     8941 gcgcccgccg gtcgtcatga tatcggtcaa taaaccggac agcgtccatt tttcctacga
     9001 gcgttatctg atcaaccgct tttacgcatc gttcgattac acgggtgtcg cggtgagatt
     9061 cctgttccgg cggtcgtcga gatgagcgtg aagaagatcg tcgaacgcgt cgtttttctg
     9121 gcgggcagat tcctttcgta tgtcgatgcg cgccgcccgc tccgggtcat ctggaacact
     9181 ctcaagggtt tcgtggacga cgactgcctc gaccgcgccg ccgccgtgtc gttctacgcg
     9241 cttctctcgt tcatcccctt cctcatcatc gtcggcgcaa tgctggggct tttcatcgac
     9301 cacctttcaa cgcccgacac gaccccgcaa caactcatcg caacggtacg gggctatctc
     9361 aagatcatca tcccctatct tcaggacgag caggtgaccg ggttcctgtc gctgtcgcgt
     9421 cataccgcct cgctctcgac catcggcttc atgtcactcg ccgtctcggc ctcgctcctg
     9481 ttctcgacgc tttccgacac gctcaaccgg atattcggca ccaaaccgat ccatttcgtc
     9541 ttctcgcggc tcgtggggtt ccttatcatt gtggccctta ccctgctcct gttcttcctt
     9601 cattatttct cggcgatggt gctgtcggtg gtcacgctcc tgcgcgacca gttcccggcg
     9661 gtccagccgg tcatcgattt cctgaccggc aacggtcttt tgtggagcat cccgatcatc
     9721 acactgttca tcctgctgtt cttcagcatc ctcatttacg ccttcacgat ggcggcccgg
     9781 atacacttcg tggcggtcat cggcggcgcg ctcctgttct cggggctttg gaacggcgcg
     9841 aaatacctgt tcaacgtgta catcacgaaa ctctcctcct acagcatcgt gtacggctcg
     9901 gcgacctggt tcgccgccgc gatactgtgg gtctattaca gcatcctgct cttgttgctc
     9961 tcaatggaat tcatccgggc gctcaacggc gagatcggca gaaagaaagg ggacgccgcc
    10021 tgaaccccgc ggtcactgtg ccggcgcttg acggcggagg aaatagaacc ggtcggggcc
    10081 gaagcggtcc ttgagcatcc ggacgcggtc ctccgaaagc cataccatcc cctccagata
    10141 gacggcccac ttgatctcgg ggaacagtat cttcaccgcg agcggcatct ccgattcctc
    10201 gatggcgctc cgctcgcgga gccgtttgat aagatcgagc gaggcatcga tgacctcacg
    10261 cggtgcctcc agcaccgcac ggacgcgccc ttccgatccg gtcgtcaggt attcggtcac
    10321 ggtgagcaca tccttggcga tgtcctctat gaccgcctcg acgcggtcga tccgttcctc
    10381 ttccatgttc cggacgatgc ggggtgatat ccggaaggcg agcgggaggg ggtgcctgag
    10441 tttgtcgacg agcggtgacg atgcatcggg tatcttattt atgacgaact cgacggcggc
    10501 gccgaccccc tcgatgacgc cgcgcaggta gtagtcgccg tcacggcctt tctgcggttt
    10561 tatgtcggtg gtcacggtcc ccagcaccca taccttctcg cgcggcgcgc ggcccgattc
    10621 ctcgatagcc gagaggtatt cctcgagatc ctcgaacccg aggttgcgcg attcgcgggc
    10681 gaaataggcg gcggggtggt tcgagacata ggtgccgagc acctcccgtt caagagcgag
    10741 ggtgtggaag atgtcccacg cgggcgtcgc gcggtcgacc gatcggagca gcgacacgtc
    10801 gtcgttccct tcgggcgccg accctgcggc ggtgtcgggc gacatgagcg agaacaagga
    10861 ggtctggccg cagaccttgt cctgatagcg tttctccccc tcgcgcaggg cgtagggcag
    10921 tatggagaac aggtcgccgc gcttgacgcc ggtgagatcg aaggcacccg ccttgatgag
    10981 gaactcgatg gcgcggctgt tgacccgggt ctggttgatg cgtgcggcga agtcgcacag
    11041 cgaggcgaag ggaccgttct tctcccgctc cttcagtatc tcctcgaccg ctccttcgcc
    11101 gacattcttg acggcgccga gcccgaaccg gatggtcccc tgttcgatgg aaaaactgag
    11161 ttccgaccgg ttgatgtcgg gcggcaggat ggcgatgtcc atggcgcggg catcggcgat
    11221 gtactcgaat atcttggcgg aatttcccgc atcggacgat atcagcgccg cgaagaattc
    11281 gctggggaag tgggtcttga gataggcggt gcggtaggcg atgaaggcgt aaacggcggc
    11341 gtgcgattta ttgaagccgt actcggcgaa cttcttcatg aggtcgaaca cgccccgggc
    11401 gacggcgagg tcgatgccct tcttgcgtga accctcgaag aaatcgaact ccgccttctt
    11461 catcgcgtcg gcctgctttt tgcccatcgc cttgcggagg gtgtcggcct gccccatcga
    11521 gaacccggcc atcgcgaccg atatctgcat gacctgttcc tgatagacga tgagcccgta
    11581 ggtctccttg agtatctcct ccacgagcgg gtggtcgtac acgacctctt cgcggccgtg
    11641 cttgcggcgg acatagttgg ggatgatcgc catcgggccg gggcggaaca gcgcgacggc
    11701 ggcgatgagg tcctcgatgc ggtcggggcg catctgtttt atcatcttgc cgaacccgac
    11761 cgattccatc tggaacaccc ctttggtggt cttcgtccag agcgtccggt aggtcgcggc
    11821 gtcgtcgagc gggatgcggg cgatgtcgaa tccgggctga ttacgccgga tgaggtccac
    11881 ggcgtgctgt atcatcgtga gcgtcttgag tccgaggaag tcgaacttca cgagccctgc
    11941 cttttcgacc atgtccttgt cgtactgggt gaccagcgcg ccgtccttgt ccttgaagat
    12001 gggcgaatac tcccacagcg gcttgtcgga gatgaccac
//
LOCUS       DAOQUE010000017        11823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_6106_length_11823_cov_4.312967, whole genome shotgun sequence.
ACCESSION   DAOQUE010000017 DAOQUE010000000
VERSION     DAOQUE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11823
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6106_length_11823_cov_4.312967"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="PKH10_01100"
     CDS             <1..1417
                     /locus_tag="PKH10_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26750.1"
                     /translation="GVFKNDEEIQLSFDLLKAQLSRKEPVLTSADTLRAAHLAAITAK
                     LGELGISWKKDPAPAAIRPADLKVTLVSGNPLKAGTEAKLLFRATYPGTIDNLIAAFD
                     TDIPFLRNLEIPFGSFSGSIEREVPVTLPEHMPWRRQPVRVDLSVDSFRSVAKTEKVE
                     IEISPLEQPDVRFALLLKESVGSMNGILESSEQVAASISVKNIGKGAILDGRLQLINV
                     NNSPELFISKGNEPLTLNPGEEKAVVFSFKVGAVTAKSRIALAVSLYDYKTKYSAGFS
                     IPFGERDLSCRYEETGDRPIILATGTPLYPDIHLSSPVAVLRSPATLRSTGRCGEAAI
                     RLQDGYWARSSDIPSTPPTNTGPLALDRRYDIAMPLLTLDRSPQTSTVPNGRLSFTVA
                     GEEIQDIFAYQNNRKVFYYRLNDGETKREFSVPLTFKEKTNKVMIVAKGKDRERTGVA
                     TRYFYYPGGKEESLSADDQEE"
     gene            1450..2256
                     /locus_tag="PKH10_01105"
     CDS             1450..2256
                     /locus_tag="PKH10_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26751.1"
                     /translation="MSILLRPGIWGLVFLIGCASTAHSGRQKETGDPSSDPMSGTEKE
                     ITITAKGGALKLDPKEYVTRELDRNNDKKADIVNVYKKVKNAEGQDDEQLFIKIIDLN
                     LDGKNDVYRFYDESGSVLKEELDLDFDGKIETTDHYLGGIVVKKEIDSQFDEKTDLWK
                     YYDDKGILIKLEEDQDGDGRVDYWESYTGGVLERVEKDTDRDGRPDVFKRAGDLKFTP
                     IIRNEGSFDGGAKATPPPTEASAPAPQETPTPPKDAPKEEKTDTPPPPDK"
     gene            complement(2296..2487)
                     /gene="rpmB"
                     /locus_tag="PKH10_01110"
     CDS             complement(2296..2487)
                     /gene="rpmB"
                     /locus_tag="PKH10_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNT26752.1"
                     /translation="MSRKCDVCGKKPMFGHNVSHANNKTQKIQQPNVQKIKVRENDGT
                     IHSISVCTRCLRSGKVVKA"
     gene            2656..3714
                     /locus_tag="PKH10_01115"
     CDS             2656..3714
                     /locus_tag="PKH10_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26753.1"
                     /translation="MRALIAGTAGLFLLACDAISTDISVSYPFDKDKPEEYSISVTEA
                     MNNLNGAILDQTQRLVGLKADTSAFPPGSDISVDTTLSLDDMLKLIAGETVSLTATVT
                     ATLNNNSQTDTQTLLFSICDFVTYKDQGVKTEFTFSDPGAEDVHMGIMNLDTYCPGKT
                     GEKPYLLITHKTTPKPIALSENQDLKDYKSLLNKIRSATLDELKLTITQPLTGVSFGD
                     SGAAGDTKISMSASLFSQRVKDCSGTGDAMRCTGIDFSEERKPADFYDDAAKGPDGTN
                     PYWIGDFGNSAMEKDSELNLIYTYNGMKILQDSIKNLDFQIGIKSWYKITPGATRPAG
                     VLKATVSATFFFSIEPLR"
     gene            3748..4674
                     /gene="lgt"
                     /locus_tag="PKH10_01120"
     CDS             3748..4674
                     /gene="lgt"
                     /locus_tag="PKH10_01120"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNT26754.1"
                     /translation="MNTALVWDIDPTIITFGPLQLRYYGILFGLTLLIGYWLFRKQLE
                     RYGYPEKIAESYLLWGVLGVIVGARLAHCFFYEPDYYLRNPAEIIKVWKGGIASHGAT
                     IGLFLTAIWFTRKHKMPFLKLGDGIVYAAAVGATLVRIGNFMNSEIVGRITDASWGMR
                     FLRNPEDLPLARSAAITANATCDLSSIDCLLPFWPVRYPSQLFEALGGLTVLALLLLT
                     LALLKGKNRPGIFSGVFLSVYFTFRFFVEFIKEFQEEYRIGSLLTMGQWLSIPFICIG
                     IWFIVRALRKEPEPRPDPIPAIADGPVKKKKK"
     gene            complement(4691..5458)
                     /locus_tag="PKH10_01125"
     CDS             complement(4691..5458)
                     /locus_tag="PKH10_01125"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT26755.1"
                     /translation="MFHNAQPIGPSEGSSALREVRFAGFQGTTLIDFPRRIASIFFVP
                     GCDLRCPYCHNGALFSVRDADHMPPDMVWAELDKRRGFIDGIVLTGGEPSLHPGLVVF
                     ARIVKERYPFEVKLDTNGLNGDFLRDMLPFLDHVAIDLKTTPERYALLGSSLSPEEVH
                     GRLVRTRELLASFPGEVEYRTTMYPPIVGDIETLRAMMVFVPPNADLYLQRFMPDHAW
                     SEEARAVTSFEPADIETLAMELRRSTGRDRIFVRTYA"
     gene            complement(5493..7734)
                     /locus_tag="PKH10_01130"
                     /pseudo
     CDS             complement(5493..7734)
                     /locus_tag="PKH10_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872410.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
     gene            8048..10837
                     /locus_tag="PKH10_01135"
     CDS             8048..10837
                     /locus_tag="PKH10_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26756.1"
                     /translation="MKMLKHLVMVLFSCYAIGLSAEEIRVVVLPKLTVENAVQNNSSL
                     EAMVIKALQEKGMRVVELSAALSSQKAAFSDTVQQGKVPQELSVLNADALASVQLTCD
                     KNSGSVLGSGVASYFCAFNSKIIRIDTGDVVYTESKDHTAHGMNALQAVQLLMKKTVG
                     TEIAAGIAQWQKNWAGDGPWGLDIVVTGLPDKQKAEALAGLLGKGDGITGASLKMFVK
                     DFSKYTVSGTGAAALKALKGRIDADAALALRVTYEAGRLIHAEFDFGRAFSRAARGYL
                     TVNAAKAPRSFVDLLTGNGAQIFDSYLRNCDYFEVKDTVFSKKDRKGLVQEAAAAGVP
                     IAIIANIANNNDLWVATIELIHAPSGRTLVTALGNDAEPFAAMDKAVRDLDVKYRKGL
                     ANPQLRKDLNLGGEAQKAAAVTDRLAIERFEAGQIFPALLPSYRQNGIGTIVLKNLAG
                     RKLTNIEVRYLIGDKVAGTHKLAAIDAGKSETVPVKLAIIPESGEYGQLAATVQYQDG
                     EIWGSKDAYAPLILHKKNAMNWAAPRTLASFIDPAHKTVRELATKAIAGDRPNKLLTR
                     QLMNAALIFGGLWHQPLKYVTDPVNTSFAADIDTVQFPGETLARLAGDCDDLTVLLAS
                     LYESVGLATVIITTPGHVLLGVESGILAGGSVVFDLPETLLVKVDGALFVPVEATAIG
                     SSFPEAWLKAADIINKSRKDLRTFRTREAWKTYPVFTVDGGKTTLAPKKNDIAALNAT
                     LDKVRAGAAEKAPAWAKELNDALTAGRAPKIEPGATGKNALAAANILWLAGDRDNAVA
                     RSGEQCAKDIVEACYNMTVMTMYDALDRNDLNIVQVNMEQQHFADAVAVLPANVVSML
                     LDNGGLGMGDEASKESKTKKKLAEVLKQARAKIQEKKAKGTINIKTSHVGGRKGADAS
                     QTKSTAGMLFWATVK"
     gene            10839..>11823
                     /locus_tag="PKH10_01140"
     CDS             10839..>11823
                     /locus_tag="PKH10_01140"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNT26757.1"
                     /translation="MEFLKKRKYLLRLVAAIVGAALGIGVAHLPFSEKIDLSFLDLSQ
                     KYGLPEREPGTVIIALDEKSFMVYGWPIGRELYAALIMTLHGAGAKRIGFDLFFVDSF
                     ESCQTTDEGRALLEENEEFLGQAAAASRTVLASFVVETIDGDPAKFPPTPPEALFDCR
                     ERDSASYLKELLPGIVKHAPEMAHVHTIPSTIDGVNRRIRPCRSLYDGCVIDLASAMT
                     DAPLDPVTCMEPVLVPFFRSYTQFPHLSMVDVLEKSDNEQGLEELSRMVKDKYVVIGA
                     TDQTLKDVGPTPLSDLDPLVVTHANRIEGILAGIAIRELPLVTIVLLSAAVG"
BASE COUNT         2556 a         3618 c         3363 g         2286 t
ORIGIN      
        1 gggcgtattc aagaacgacg aggagataca actctctttc gacctgctca aggcgcaact
       61 ttcgcgaaaa gagcccgtcc tgacctcggc cgataccctg cgagccgctc accttgccgc
      121 catcactgcc aaactcggcg aactaggcat atcgtggaaa aaagaccctg ctccggcggc
      181 gatccggccg gccgacctga aggtgacgct ggtctccggc aacccgctca aagcaggtac
      241 cgaggcgaag ctcctgttcc gcgccaccta tccgggcact atcgacaacc tcatcgccgc
      301 cttcgatacc gatatcccgt tcctgcgaaa tctcgagata ccattcggct ccttctcggg
      361 aagcatcgag cgggaggtgc cggtgacctt gccggaacac atgccgtggc ggcgccagcc
      421 ggtccgtgtc gacctttctg tggacagttt ccgctccgtc gcgaagaccg agaaagtgga
      481 gatagagata tcgcccctgg aacagcccga tgtccgtttc gccctgctcc tcaaggaatc
      541 ggtcggatca atgaatggca tcctcgaatc atccgaacag gtggcggcat ccatatcggt
      601 caagaacatc ggcaaaggcg ccatcctcga cggccggctt caactgataa atgtcaacaa
      661 cagtccggag cttttcatca gcaagggcaa cgaaccgctg accctcaatc cgggtgagga
      721 aaaggcggtg gtcttctctt tcaaggtagg cgccgtcacg gccaaaagcc ggatagccct
      781 cgccgtttcc ctgtacgact acaagaccaa atattcagcc gggttctcga taccattcgg
      841 tgaacgggat ctttcctgcc gttacgagga gaccggcgac cgcccgatca tcctcgcgac
      901 cggcaccccc ctgtatcccg atatccacct ctcctcgccg gtcgccgtgc tccgctcccc
      961 cgctacgctg cggagtaccg gacggtgcgg cgaagcggcg atacgcctgc aggacggcta
     1021 ttgggcccga tcctcggaca taccctcgac gcctccgacc aacaccgggc cactggctct
     1081 cgaccgacgg tatgacatcg cgatgcccct cctgacgctt gatcgctcac cgcagaccag
     1141 taccgtcccc aacggacgcc tgtcgttcac cgttgccggc gaagagatac aggacatctt
     1201 cgcctatcag aacaaccgta aggtgttcta ctaccgactg aatgatgggg agaccaagcg
     1261 ggagttctcg gtccccctca ccttcaagga aaaaacgaac aaggtcatga tcgtggcgaa
     1321 gggaaaggac cgtgagcgga ccggtgtcgc cacacgatac ttctattatc ccggcggcaa
     1381 ggaggaatcg ctttccgccg atgatcagga ggagtgaaca tgcaggtaac ggcgaataag
     1441 ggggttcaca tgagcatctt gttgcgaccg ggcatctggg gcctcgtctt cctcatcgga
     1501 tgcgcctcga ccgcccattc cgggcgtcag aaggagaccg gggatccttc ctccgacccg
     1561 atgagcggga cggaaaagga gatcaccata acggccaaag gcggcgcgct caaactcgat
     1621 ccaaaggaat atgtgacgcg cgaactcgac cgcaacaacg ataaaaaggc cgacatcgtc
     1681 aatgtctaca agaaggtcaa gaacgccgag gggcaggacg atgagcaact cttcatcaag
     1741 atcatcgacc tcaacctcga cgggaaaaac gatgtttaca ggttttacga cgagtcaggc
     1801 tcggtgctca aagaggaact cgatctcgat ttcgacggaa agatagagac caccgaccat
     1861 tatctcggtg gcatcgtggt gaaaaaggag atagactcgc agttcgacga gaagaccgac
     1921 ctgtggaagt actacgatga caaggggata ctcatcaaac tcgaagagga tcaggacggc
     1981 gacggacgcg tcgactactg ggaatcatac accggcggcg tgctggagcg ggtcgagaag
     2041 gacaccgacc gcgacggacg tcccgatgtg ttcaagcggg cgggcgacct caaattcacg
     2101 ccgatcatac ggaacgaggg ctcgttcgac ggcggcgcca aggcgacacc cccaccaacg
     2161 gaggcctcgg ccccggcacc tcaagaaacg cccactcccc ctaaagacgc cccgaaagag
     2221 gaaaagacag acacgccgcc tcctcccgac aagtgacctt atgcgcatga ccagagaaat
     2281 aaggcctcga gaatactagg ccttaacgac ctttccggaa cgaagacacc gtgtacagac
     2341 cgatatcgaa tggatcgtgc cgtcgttctc gcgaaccttt atcttctgca cgttgggctg
     2401 ctggatcttc tgggtcttgt tattcgcgtg gctcacatta tgaccgaaca tcggcttctt
     2461 gccacacaca tcgcacttac gggacatcgt cttctccctc atacctaaag acaataaaac
     2521 aggggcacga tactcatgat cgaccgaaaa tcaatctttt ttctttgtcc cagagtatgc
     2581 ccctgcttgc ttttcgactt ctttcgggta ctataggcac ggtcaagtac ggtatctttt
     2641 gaatgggagg ttctcatgcg cgcgctcatc gcggggacgg cggggctttt tttgctcgcc
     2701 tgtgacgcca tcagcaccga tatctcggtc agttatccct tcgacaagga caagccggag
     2761 gaatactcca tcagcgtcac cgaggcgatg aataacctca acggcgccat cctcgaccag
     2821 acccagcgcc tcgtggggct caaagccgac acctcggcgt tccctcccgg gtccgatatc
     2881 tcggtcgata cgaccctttc gctcgacgac atgctcaaac tcatcgcggg agaaacggta
     2941 tccctcacgg ctacggtcac cgcgaccctc aacaataaca gtcagaccga tacgcagacg
     3001 ctgctgttca gtatctgcga cttcgtcacc tacaaggacc aaggggtcaa gaccgagttc
     3061 accttcagcg acccgggggc cgaggatgtc cacatgggga tcatgaatct cgatacctac
     3121 tgccccggca agacggggga aaagccctat ctgctcatca cgcataagac caccccgaaa
     3181 ccgatagcgc tctccgagaa ccaagatctc aaggattaca agtcgctcct caacaagata
     3241 cgctcggcaa cgctcgacga actcaagttg accatcaccc aaccgctcac cggcgtctcg
     3301 ttcggcgact cgggcgccgc cggggacacc aagatatcga tgagcgcctc tcttttctcc
     3361 cagagggtca aggactgttc cggtaccggc gacgcgatgc gatgcacagg catcgacttc
     3421 agcgaggaga ggaagccggc cgatttttac gatgacgccg cgaaaggccc cgacggcacc
     3481 aatccctact ggataggcga cttcggcaac agcgctatgg aaaaagacag tgaactgaat
     3541 ctcatctaca cctacaatgg tatgaagata cttcaagatt ccatcaagaa tctcgacttc
     3601 cagatcggca tcaagtcgtg gtacaagata acgcccggcg cgacccgacc ggcgggagtt
     3661 cttaaggcga ccgtgagcgc gaccttcttc ttcagcatcg aacctctgcg gtgatgtcgt
     3721 cataaccact atcgtaaagg agttctcatg aacaccgctt tagtctggga catcgacccg
     3781 acgatcatca cgttcggccc cctgcaactg cgctactacg gcatcctgtt cggactcacg
     3841 ctcctcatcg gctactggct gttccgcaag caactggaac ggtacgggta ccctgagaag
     3901 atcgccgaaa gttacctgct gtggggcgtg ctcggcgtca tcgtcggggc gcggctggcg
     3961 cattgcttct tctacgagcc cgattactat ctgcgtaatc ccgccgagat catcaaggtc
     4021 tggaaaggcg gcatcgcgag ccatggggcg accatcgggc tgttcctcac ggcgatatgg
     4081 ttcacgcgga aacacaagat gcccttcctc aaactgggcg acggcatcgt ctacgccgcg
     4141 gcggtcgggg ccacgctcgt ccgcataggc aacttcatga acagcgagat cgtggggcgc
     4201 atcaccgacg cctcatgggg catgcgcttc ctcaggaatc ccgaggacct ccctctcgcc
     4261 cgctcagcgg ccatcactgc gaacgccacc tgtgaccttt cctcgatcga ttgcctgctc
     4321 cccttctggc cggtccggta tccgagccaa cttttcgagg cactcggcgg acttaccgtg
     4381 cttgcgctgc tattgctcac gctcgctctg ctgaaaggga agaaccgtcc ggggatattc
     4441 tcgggcgtct ttctttcggt ctatttcacc ttccgtttct tcgtggagtt catcaaggaa
     4501 ttccaggagg agtatcgtat cggctctctg ctcacgatgg gacagtggct cagtatacct
     4561 ttcatctgca tcggcatctg gttcatcgta cgcgccctga gaaaagagcc ggagccgaga
     4621 cccgacccga taccggcgat cgccgacggt cccgtcaaga aaaagaagaa gtaacggcgg
     4681 acctatccgg tcaggcgtag gtgcggacga agatgcggtc ccgccccgtg gagcgccgca
     4741 actccatcgc caacgtttcg atatctgccg gctcgaacga tgtgaccgcg cgagcctctt
     4801 cggaccacgc gtggtcgggc atgaatcgct ggagatacag atcggcattc ggcggcacga
     4861 agaccatcat ggcgcgcagt gtctcgatgt caccgacgat cggcggatac atagtggtgc
     4921 gatactcgac ctctcccggg aacgaggcaa gcagttcgcg cgtccggacg agacgaccat
     4981 gcacctcctc gggtgacaac gacgaaccga gcagcgcata gcgttcaggc gtcgttttaa
     5041 ggtctatcgc cacatgatcg aggaacggaa gcatgtcgcg caaaaaatcg ccgttcagcc
     5101 cgttcgtgtc gagtttcact tcgaacgggt agcgctcctt gacgatccgg gcgaaaacga
     5161 ccagccccgg gtgaagtgac ggttcgccgc cggtcaacac gataccatcg atgaaaccgc
     5221 gccgtttatc gagttcggcc cagaccatat ccggtggcat atggtccgcg tcgcgtacag
     5281 agaagagcgc tccgttatgg cagtatggac agcgcagatc gcaccccggc acaaagaata
     5341 tcgacgcgat gcgccgaggg aagtctatca gcgtcgtccc ttggaagccg gcgaaccgaa
     5401 cctcgcgaag ggcggaagaa ccctccgacg gaccgatggg ctgagcattg tggaacatcg
     5461 agatcaccgc gggcgaccgg cggaaggccg gatcacgcct ccgctacggc gcactgctcg
     5521 gcggcgacgg gaacggcgac gaccgtctcg cgaccgtcgg tcgtcacctt gaaggtcttg
     5581 cgcgcttcga actcctcttt tttccccttg ttccagttct ggaccggacg gtaatacccg
     5641 accacgcgac tgtacacctc ggtgcgctct ccacaaacgg catttttctt catggggctc
     5701 tccttgtcac tgttgtatgg acatctctgt cttcgttgtt tcctcggaaa catggtgcgg
     5761 gcaggtgcgg tgctcgccgg cgacatagcc gtgcaccgga cagacgctga aggtggggga
     5821 tatcgtgaaa tagggcagat ggaagttctt cgatatcttc tgtacgagcc gcttgcatat
     5881 ctcgcccttc tctatcttct cgccgacgaa caggtgcacg acggtgccgc cggtgtaact
     5941 cgtctggagt tcatcctgat gttcgagcac ctcgaagatg tcgtcggaga aatcgaccgg
     6001 caactggctc gaattggtgt agtacggctc gtcgtggcct gaagtgacga tgtcgccgaa
     6061 atcctttttg tccttgagtg cgaggcggta tgaggtccct tcggccggcg ttgcttcaag
     6121 gttgtacagg tgactcgtct gctcttggaa ctcctgcagg acatcgcgca tgaaactcag
     6181 ggtacgaagg gcgaacgagc gaccctcggg cgtttcgatg ccgaccccga gaagattaag
     6241 cgccgcctcg tgcatgccaa cgagcccgat ggtcgaaaaa tggttgtccc agaacgcccc
     6301 gcgctgtttc gctatgtcgc caagatagta tttcgagaag ggatagagat cctcacgggt
     6361 gtattgctcg agcaccttac gctttatctc caatgaatcc ttccccagtt ccatccggtg
     6421 acgcacatta tcccggaact cgttctcgtc cttggaaaga taggccatac gcggcaggtt
     6481 tatcgtcacg acgccgatgg agccggtgag cggattactg ccgaacaggc cgccgccccg
     6541 tttgcgcagt tcgcggttgt cgagccgcag acggcaacac atcgaccggg cgtcgtcggg
     6601 cgacatgtcg gaattcacga agttcgcgaa atagggaatg ccgtatttgg cggtcatgtc
     6661 gaacaaccgc ttgacgacgg ggcggtccca ctggaagttc tgcgtgatgt tgtaggtcgg
     6721 tatcgggaag gtgaatatgc gccctttcgc gtcaccctcg atcatcacct cgatgaaggc
     6781 ctgattgaac atatccatct cggcctggaa atcgccgtac ttcgcgtcct gcatgacgcc
     6841 gccgatgatg accgcctcct gcgcgatctg acccgacggg acgatatcca tcgtgatgtt
     6901 ggtgaacggc gtctggaagc cgacgcgggt cggtatgttc atgttgaaca ggaactcctg
     6961 tatcgcctgt ttgacctgtt cgtaggtaag cccgtcgtag cggatgaacg gcgcaaggta
     7021 ggtgtcgaaa ttcgagaagg cctgcgcgcc cgccgattcg ccttgcaggg tatagaagaa
     7081 attcacgatc tgtccgagca cggtgcgcag gtgcttggcc ggcttcgact ctatcttacc
     7141 aggcgctccg cggaaaccgt cggtcaggag tttcttgatg tcccacccgc aacaatagac
     7201 cgacaacaat ccgaggtcgt gaatatgcat ctccgccttc tcatgggctt ctcgcaccgc
     7261 ggccggatag accttgttga gccagtagtg tgtcgtgaat gcggtggaga tatagttgtt
     7321 cagcccctgc agggaatagg tcatgttcga attctcattg accttccaat cgtcgcgcat
     7381 aaggtagttg tcgagtagtt tgttcgcctc gttgaacacc tccttagcgc ggcggacctc
     7441 ggagcgtcta tcgcgataca ggatgtacgc cttcgcggtc ttggcgtggc cttcttctat
     7501 gagtatcttc tcgacgagat cctgtaccga ctcgaccgac ggatacccgc cgtccttata
     7561 taatatctcg agatagccga ccaccttggc ggcgatcctc tcggccatcg tccggtccgt
     7621 accgccgacc gactgcaccg ccttgaagat ggcatcggcg atcttctgcg ggttgaattc
     7681 tacgagcaca ttgtttcttt tcctgatgaa tcggatggac gaagcagatt gcatagcgcg
     7741 tctcccctcc ggagcgttta tttaacgata ccgatgaaac tcgcttgcac cacaacatat
     7801 tgcgtggcgc ttgctcatac tacaccaccg gctgtaagaa tcaatttttt ggtcattttt
     7861 tttctttaac gatttcttcc ggttgttccg aaacatcttt tcaccctgaa aggatggaca
     7921 agatggccct ttttttgtct ggagcgcggt aaaaaattcc tttcctgaga catcgtgtta
     7981 tactgcctcc ggtctttggt gagaccgctg ttccgtcaag gtccggacaa gggttacgga
     8041 ggaaacgatg aagatgctga aacacttggt gatggtgctg ttctcgtgtt acgcgatcgg
     8101 cctgagcgcc gaagagatcc gcgtggtggt gctccccaaa ctaaccgtcg agaacgccgt
     8161 ccagaacaac tcgtcgcttg aggcgatggt catcaaggcg ctgcaggaaa aaggaatgcg
     8221 ggtcgtcgaa ctttccgccg ccctttcttc ccagaaagcc gctttttccg acacggtgca
     8281 acagggcaag gtgccgcagg aactctccgt gctgaacgcc gacgcgctcg catcggtgca
     8341 actgacctgc gacaagaatt ccggctcggt gctcgggagc ggcgtcgcca gttatttctg
     8401 cgcgttcaac tcgaagatca tccgcatcga caccggcgat gtggtataca ccgaatcgaa
     8461 ggatcacacc gcgcacggca tgaacgccct gcaggcggtc caactgctca tgaagaagac
     8521 ggtggggacc gagatagcgg cgggtatcgc ccagtggcag aagaactggg cgggggacgg
     8581 cccgtggggt ctcgacatcg tggtgaccgg gctacccgac aagcagaagg ccgaggctct
     8641 cgcaggcctg ctcggcaaag gtgacggcat caccggcgcg agtctgaaga tgttcgtgaa
     8701 ggatttctcc aaatacaccg tgtcgggcac cggtgccgcc gcgctcaagg cgctcaaagg
     8761 ccgcatcgac gccgacgcgg cgctcgcgct ccgggtcacc tacgaggcgg ggcggctcat
     8821 ccatgccgag ttcgatttcg gacgcgcctt ctcgcgcgcc gcccgcggct atcttacggt
     8881 gaacgccgca aaggcgcccc gctcgttcgt cgacctcctg acgggtaacg gcgcccagat
     8941 attcgattcc tacctgcgca actgcgacta cttcgaggtg aaggataccg tcttctcgaa
     9001 aaaggaccgc aaaggactcg tgcaggaggc ggccgcggca ggcgtcccga tcgcgatcat
     9061 cgcgaacatc gcgaacaaca acgacctgtg ggtcgccact atcgaactca tccacgcccc
     9121 gtcgggccgt acgctcgtga cggcgctcgg caacgacgcc gagccgttcg ccgcgatgga
     9181 caaggcggtg cgcgacctcg acgtgaaata ccgcaaagga ctcgccaacc cgcaactgcg
     9241 caaggacctc aacctcggcg gcgaggcgca gaaggcggcg gcggtgaccg accggttggc
     9301 catcgagcgg ttcgaggcgg ggcagatatt cccggcgctc ctcccctcct atcgtcagaa
     9361 cggcatcggc accatcgtgc tgaagaatct cgcgggccgc aaactcacca acatcgaggt
     9421 acgctatctc atcggtgaca aggtcgccgg cacccacaaa ctcgcggcga tcgatgcggg
     9481 caagagcgaa acggtcccgg taaaactggc gatcataccc gaaagcggcg aatacggtca
     9541 gctggcggcg accgtgcagt accaggacgg cgagatatgg gggagcaagg atgcctatgc
     9601 gccgctcatc ctccacaaga agaatgcgat gaactgggca gccccccgga cgctcgcctc
     9661 tttcatagac ccggcgcaca agacggtccg cgagctcgct accaaggcga tcgcgggcga
     9721 tcgccccaac aaactcctga cccggcaact tatgaacgcg gcgctcatat tcggcgggct
     9781 ctggcaccaa ccgctcaagt atgtgaccga tccggtcaac accagtttcg ccgccgacat
     9841 cgacaccgtg cagttccccg gcgaaacgct cgcgcggctc gcgggcgact gcgatgacct
     9901 gacggtgctg ctcgcctcgc tctacgagtc tgtgggactt gcgaccgtca tcatcaccac
     9961 accgggccac gtcctgctcg gcgtggagag cggcatactc gcgggcggca gtgtcgtgtt
    10021 cgatcttccc gaaacgctgc tcgtcaaggt tgacggcgcc ctgttcgtcc cggtcgaggc
    10081 gaccgccatc ggctcgtcgt tccccgaagc gtggctcaag gcggccgaca tcataaacaa
    10141 gtcgcgtaag gacctgcgga ccttccgtac ccgcgaggcg tggaagacct acccggtctt
    10201 tacggtggac ggcggcaaaa cgacgctcgc accgaaaaag aacgatatcg cggcgctcaa
    10261 tgcgacactc gataaggtac gcgccggagc ggccgagaag gcacctgcgt gggcgaagga
    10321 attgaacgat gcgctgaccg cgggccgtgc cccgaagata gagcccggag cgaccggcaa
    10381 gaacgccctt gcggccgcaa acatcctgtg gctcgcggga gaccgcgaca acgccgtcgc
    10441 ccggtcgggt gaacagtgcg ccaaggatat cgtcgaggcc tgctataaca tgaccgtcat
    10501 gacgatgtac gacgcactgg accgcaacga tctcaatatc gtgcaggtca acatggaaca
    10561 gcagcatttc gccgatgccg tggcggtgct cccggccaat gtcgtctcga tgctcctcga
    10621 caacggcggg ctcggcatgg gggacgaggc gtcgaaggaa tcgaagacca agaagaaact
    10681 ggccgaggtg ctcaagcagg cacgggccaa gatacaggaa aaaaaggcga aaggcacgat
    10741 caacatcaag acctctcatg tcggaggccg aaaaggggcc gatgcgtcgc agaccaagtc
    10801 gaccgccggc atgctgttct gggccacggt gaaataaagt ggagttcctc aaaaagagga
    10861 aatatctcct gcggctcgtc gccgcgatcg tcggcgccgc gctcggcatc ggcgtcgccc
    10921 acctcccctt ttccgaaaag atcgatcttt cgttcctcga cctgtcgcag aaatatggtc
    10981 tgcccgagcg cgagccgggc accgtcatca tcgcactcga cgagaagagt ttcatggtct
    11041 acggctggcc catcggccgc gaactctacg ccgcactcat catgacgctt cacggggccg
    11101 gggcgaaaag gatcggcttc gatctttttt tcgtcgacag tttcgagtcg tgtcagacca
    11161 ccgacgaggg cagggcgtta ctcgaggaga acgaggagtt cctcggccaa gcggccgcgg
    11221 cgagccggac ggtgctcgcc agtttcgtcg tcgaaacgat cgacggcgac ccggcgaaat
    11281 tcccaccgac accgcccgag gcgctgttcg actgccgcga gcgggacagc gccagttacc
    11341 tcaaggaact gctcccgggc atcgtgaaac atgcccctga gatggcccat gtccacacga
    11401 taccttctac catagacggg gtgaaccgcc ggatacgccc atgccggtcg ctttatgacg
    11461 gctgcgtgat cgatctcgcc tcggcgatga ccgacgcgcc gctcgacccg gtcacctgca
    11521 tggaaccggt cctcgtcccc ttcttccgct catacacgca gttcccccac ctttcgatgg
    11581 tggatgtcct cgagaagtcg gacaacgagc agggtctcga agaactttcc cgtatggtca
    11641 aagacaagta cgtcgtcatc ggggcgaccg accagacact caaggatgtc gggccaacgc
    11701 cactatcgga cctcgacccg ctggtcgtga cccacgccaa ccgcatcgaa gggatactcg
    11761 ccggcatcgc gatacgggaa cttcccctgg taacgatagt gcttctctcg gccgccgtgg
    11821 gac
//
LOCUS       DAOQUE010000018        11812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_6117_length_11812_cov_4.864591, whole genome shotgun sequence.
ACCESSION   DAOQUE010000018 DAOQUE010000000
VERSION     DAOQUE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11812
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6117_length_11812_cov_4.864591"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="PKH10_01145"
     CDS             complement(<1..1326)
                     /locus_tag="PKH10_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNT26758.1"
                     /translation="MFIPTTRDEMARRHWNALDIVLVTGDAYIDSPYNGAAIVGHHLI
                     AHGFRVGIIAQPVADGPSDITRLGEPLLFWGVTAGSVDSMVSNHTALGKRRREDDLTA
                     GGVNDRRPDRASIVYTGLIRRYFKHTVPVVLGGIEASLRRVAHYDLWSDTIRRSILFD
                     AKADILVYGMAERAIVELAAALRDGREWRDILGLCYIADVPVEQFLGLPPYEKAAASD
                     KTFADMFLAFYRNNDPLTARGMHQRHGDKWLIHNPPQPALTPEELDAVYELPYEYDAH
                     PSEKERGEVRALETIKFSVTTHRGCYGECSFCAIHLHQGRRVVSRSEDSIVREITRFL
                     KMKRFTGVIYDLGGPTANMYGIDCARKSKSGACREKRCLHPSVCRELAPDHRRQLALL
                     KRVASLPGVKRVFVASGVRHDLVLADKEHGEPYLAALVSAHISGQMKIAP"
     gene            complement(1383..2291)
                     /locus_tag="PKH10_01150"
     CDS             complement(1383..2291)
                     /locus_tag="PKH10_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26759.1"
                     /translation="MNGTEHLPQLAELVAMDQPLAVRAEVETTMQMAYPGLDTRRVRE
                     VFEHILALFDGRVPGYRPCNTEYHNLQHTTDAFIAMARLIHGAVVEGNIVSEKNVELA
                     LIATILHDVGYIQQEGDAGSGAKYTQNHIDRSADFLSAYLRAKGFDEGDMADGRDMLA
                     CTGFTVRIQDIRFRSFESRVLGMMLGTADLLGQMADRAYLEKISLLYREFKEAKIPGI
                     AEEIDLYLNTLSFYELTQKRFANDLGGVNRFMRSHFRARWGVDADLYAVSIERQMNYL
                     REILEHFRPDYRKHLRRWRPIRKEGS"
     gene            2418..3203
                     /locus_tag="PKH10_01155"
     CDS             2418..3203
                     /locus_tag="PKH10_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT26760.1"
                     /translation="MGIGTLRIARNGLALFIIIIFFSLCNVTLWAVTRDTVLQRRRIQ
                     RSTRFWIRTTLNALNISYTLKGAENLRNDRNHLIIANHISYVDVLLLYALRPLTGVAS
                     VEVEVGGLDGFLAKMGGSLFIERRSVRHLRGELAKVTALLRDRFDVLIFPEGTTTDGK
                     SPLKPFRTAFFAAAADAGTPILPVAICYETIDGRPAREDDLLDAVAYYGDISFLSHAK
                     KFLALKSITATITVLPPVEAASAHRDQLGVTLREQIQTARDAI"
     gene            3270..4127
                     /gene="panC"
                     /locus_tag="PKH10_01160"
     CDS             3270..4127
                     /gene="panC"
                     /locus_tag="PKH10_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNT26761.1"
                     /translation="MLTVRTAAELRTARAALAGSVCFVPTMGALHAGHARLMEVAKKK
                     ADHLVVSIFVNPTQFGPNEDFSKYPRAEETDLALCEAAGADIVFLPKVAEIYRTEPTV
                     RVTVDPKLSGILCGAQRPGHFDGVAQVVAILFNLVRPDSACFGEKDHQQLAVIRKLVE
                     DLHFPVAIVPVATVREPDGLALSSRNRYLSPAERAAAPAIYRALLYCKGRAEQIRFDR
                     IALPVGKVEEEGRALILNDIPGAQIDYFEIRHTDTLEPTHFIMNRSRAFAAVRVGATR
                     LIDNLLLNG"
     gene            4131..5294
                     /locus_tag="PKH10_01165"
     CDS             4131..5294
                     /locus_tag="PKH10_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT26762.1"
                     /translation="MARIAQAVTAQVAYPFATLAAKKRAMEVRGHTVIDLSIGDASLP
                     APDAAVTALRERSLLAAHHHYSSYNGILPLRRAIADWIDRRFGVAIDPEREVTALMGS
                     KEGLFRFPQAVLDPGDAALHPDPGYPVYPLAIRDAGGRPVAMDLSEKNGFLPDLGAIP
                     EKTRRDVRMIIVNFPSNPTGATAGPAFYGELYRMAVRYDWIVVSDNAYCEIYDGAPPR
                     AALEFDPKHERTVEFFSFSKTYSMTGWRLGWACGNADLVQALVKVKGLQDSAPFEPVQ
                     FAGIAALSLSDADLAPTRRFYADNRALMKKALDAAGIPYFDAQAGFYLWARVPAGLSA
                     AGLTDRLLEERRVLVTPGTAFGPNGEGWFRIAATRHPDEMTEAARRISQGLPA"
     gene            5291..5776
                     /gene="folK"
                     /locus_tag="PKH10_01170"
     CDS             5291..5776
                     /gene="folK"
                     /locus_tag="PKH10_01170"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNT26763.1"
                     /translation="MRYLLSLGSNIGDRTTNIDAALRLLDERGVTIQRRSSLYRTAPV
                     EAPPQEDFVNAVALVETPLSPEELLDLTTAIETALGRTRTAKNGPRIIDIDIVLSAAG
                     TYRSPRIEIPHPRWRQRRFVVEPAREIADGTPFADPVAAIDTRSLTHQRIERIPTEAL
                     P"
     gene            5773..6093
                     /locus_tag="PKH10_01175"
     CDS             5773..6093
                     /locus_tag="PKH10_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26764.1"
                     /translation="MKTRLVGKLLDRMPIPDELKDIIGDQLDLSAIISREVATQLLEH
                     GGKMKQEAKAILARELAGFLEKVDVDKALTNALENLELEIRISFRRKDKKTEPAPKGR
                     GKAR"
     gene            6097..7065
                     /locus_tag="PKH10_01180"
     CDS             6097..7065
                     /locus_tag="PKH10_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNT26765.1"
                     /translation="MQRSYTILREFLADAVRTSMILFKIMIPVSIVIKILKETGLIEL
                     IGAGFAPLMELIGLPGAMGLVWVTGMVTNLYGGILAFVAISADHPLTTAQVTVLTTMM
                     LVAHSLPVELQVARKAGASLPAILAVRVGGGYIIGTLLYLGYRLTGTLGGPSTISWAA
                     TAPADPSLMAWALGELRNYGMIFLVICALLFLMALLKKFGVIAFMDRLFGPVLSFIGI
                     GREVTTLTIIGMTLGLAYGGALIIREAQTGHIARRDIFYSMALLSLLHSFIEDTLLMV
                     SLGGHFSGLLIVRALFAITAVFAMVRLTAKLSDERFARLLLSRAVS"
     gene            complement(7046..7834)
                     /locus_tag="PKH10_01185"
     CDS             complement(7046..7834)
                     /locus_tag="PKH10_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT26766.1"
                     /translation="MSDNLLHILSSGSKGNAVIVESGDELLLIDQGLSFKCFNGRCAA
                     AGIDPARIVGIVITHEHIDHVSGVPLTAHRLHLPVYAAPVVIEYLAESGGRYEFETRE
                     IAPRRPVTIGRFVVEPFPVMHDARDPYGFTVTLPNGERLTLVTDTGIVTQRIFAQIAA
                     SEYLIIEANHDPDMLYRNPRYPPDLKQRIRGAQGHLSNEQCLEALEKLPHDKLKKVIF
                     AHLSEENNSPELVRHSAKECFGRTRFTGESFIAPQAGPLTIPLD"
     gene            complement(7824..8336)
                     /locus_tag="PKH10_01190"
     CDS             complement(7824..8336)
                     /locus_tag="PKH10_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26767.1"
                     /translation="MSGREREAAQAVPFLGQEFLLWLYWRSSSDPYFFLDNLGLGSID
                     LRLEEQITLESLRGEGYRETIQTQEVALSDDIRDSVRTGRIPVAARVKVSRGKAEWQF
                     ILTALPLAMRSVKLPAPEKSEDDETMHLRLIQMEEIDRIMRALFKIFLAERTNPAFVN
                     DIRKFLGLER"
     gene            complement(8333..8941)
                     /locus_tag="PKH10_01195"
     CDS             complement(8333..8941)
                     /locus_tag="PKH10_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26768.1"
                     /translation="MSKLRSGRVSFMTFIPEKQPPDVTKLRKLLREHRFLPLAPEDVR
                     DESVGWVDPLLSFETEEFPNCYFRDYYLFSLRIDRYGSGGSQLRPFLEEAEFRFKEEH
                     KIERIPAHQKKELRETAARRVRVKTLPRTVIAEVAWNLGENRIYLFSQSSGVVARFVD
                     AFEKTFAISVAHSLIVDAVDEIDKPEELTSIIGDLWDLGGDA"
     gene            complement(8938..9261)
                     /locus_tag="PKH10_01200"
     CDS             complement(8938..9261)
                     /locus_tag="PKH10_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26769.1"
                     /translation="MYYFATFTTHFTQKNVPGVRNLIKELEEVGLRHLVKTGEETFAE
                     LPANTWIGEYDTEDKEELKNLLYAEVKRLFEKNKLAGTIFISISEKAVVGVAELSGPD
                     AKSAK"
     gene            complement(9341..10663)
                     /locus_tag="PKH10_01205"
     CDS             complement(9341..10663)
                     /locus_tag="PKH10_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26770.1"
                     /translation="MIKYLMFLAVMVLMIACGVKEESICNNGEWKAEAGVVSECVENI
                     WVMRRITRQWGTLYNDAGSALTMDAKGNFYVAGGAIDDNEEDKYDSLTLIKWNQDGTK
                     AWSRSWSTDKLEMTTALATDPHGGIYVVGSTTGKIDDNVCAGRTDAFLIKWNDSGVKE
                     WSKEWGTNEDEIAYAVTLDATSNIYITGMTRGSLDGNVHDGRLCGEYGDGAPLPCGDI
                     FLTKWDKYGTKLWTRQWGSSGGNEGRAISVDSTNNVYVAGRIGGEQILVKWSSDGEKL
                     WSKTFDWMQIYSLLADSTGNVYVSGNICDENISDCHIFLTKISKDGDIEWTKRWGNYV
                     EGLSPQALSLFVANDSEYVYVTGSIWGDWEERESSGGHDVFITKWDAKGNNNMIERLW
                     GTEEYDAGTYIKVDGAGDIYVVGWTNGGIDGNATVGGKDLFFTQWLVE"
     gene            complement(10747..11433)
                     /locus_tag="PKH10_01210"
     CDS             complement(10747..11433)
                     /locus_tag="PKH10_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26771.1"
                     /translation="MKNIPFRHDLPALAGLALSAFFAFRDGWTLPEFCWSAWLAGLFF
                     SYACVVVAPVQIILFSGREITRIRDRITQLKRVPDGALKAGMIVVALVIGGVSFYVYS
                     YLFGFYGIFLSVFAEMEPLTFFGRNGFINSDFYTPVVYLIDRFWPMVAGVLVGNFALF
                     LDPDPWGKAVMPFKSKQIVRIHLMVVLMPFFTLAAWAVVGDRYHFPVILLLMALFYLI
                     PEGSRRPEAG"
     gene            complement(11449..>11812)
                     /locus_tag="PKH10_01215"
     CDS             complement(11449..>11812)
                     /locus_tag="PKH10_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT26772.1"
                     /translation="APSCDNVVMAGPDDWGCDAGFSYPVGSKETGLSAYGLFDMAGNA
                     REWLEDDWHDDYDDGTRPDDGTAWVDETRPAQRVMRGGSFMIGSDKYVQFMTYARFGF
                     PVENTDRSGGFRCARTPE"
BASE COUNT         2260 a         3747 c         3429 g         2376 t
ORIGIN      
        1 cggcgctatc ttcatctggc ccgagatatg ggcggagacg agcgcggcaa ggtacggctc
       61 tccatgctct ttgtcggcga gcacgagatc atggcggacg ccgctcgcga cgaacaccct
      121 cttcacgccg ggcagagagg cgaccctttt caggagtgcg agttggcgtc gatggtcggg
      181 cgccagttcc ctgcataccg acgggtggag acaccgcttc tcccggcaag cgccgctttt
      241 cgacttgcgc gcgcagtcga tgccgtacat gttggcggtc ggcccgccga gatcgtagat
      301 gaccccggtg aaccgtttca tcttcaggaa tcgcgttatc tcgcggacga tggagtcctc
      361 agagcgcgac acgacccggc gtccctgatg gaggtgtatc gcgcagaacg aacattcgcc
      421 gtaacagcca cggtgggtgg tcaccgagaa cttgatcgtt tcgagcgccc gcacctcgcc
      481 gcgttccttt tccgacgggt gggcgtcgta ctcgtacggc agttcgtaca ccgcgtcgag
      541 ttcctcgggc gtgagggccg gttgcggcgg attgtggatg agccatttgt caccgtgtcg
      601 ctgatgcatg ccccgcgccg tcagtgggtc gttgtttcgg tagaaagcga ggaacatgtc
      661 ggcaaatgtc ttgtcgctgg ccgccgcttt ctcgtacggc ggcaggccga ggaattgttc
      721 gacgggcacg tccgctatgt agcataggcc gaggatgtcg cgccactccc gcccgtcgcg
      781 gagcgccgcg gcgagttcga cgatcgcccg ctcggccatg ccatagacga ggatgtcggc
      841 cttcgcgtcg aacaggatgc tccgccgtat ggtatcggac cagagatcgt agtgggcgac
      901 gcgccgcaga ctcgcctcga tgccgccgag tacgacgggg accgtgtgct tgaagtatcg
      961 ccggatgagc ccggtataga cgatgctcgc ccgatcgggg cgccggtcgt tgacgccgcc
     1021 cgccgtgaga tcgtcctcgc gtctccgttt cccgagcgcg gtgtggttcg acaccatcga
     1081 atcgaccgag ccggcggtga cgccccagaa caggagcggc tcgccgagcc gcgtgatatc
     1141 cgacggtcca tcggccaccg gctgtgcgat gatgccgacg cggaacccgt gcgcgatgag
     1201 gtgatggccg acgatcgcgg cgccattata cggggaatct atgtaggcgt cgcccgtcac
     1261 gaggacgatg tcgagcgcgt tccagtgccg ccgggccatc tcgtcgcgtg tcgtcgggat
     1321 gaacatgcct gaaaaccccg taccgtggct tgaaggttat gaccgaacgg gggcccctgt
     1381 cgtcaagagc cctctttccg gatcgggcgc caccgtcgca ggtgtttgcg gtaatcgggc
     1441 cggaaatgtt cgagtatctc gcgcagatag ttcatctgtc gttctatcga gacggcatac
     1501 aggtcggcat cgacgcccca tcgggcgcgg aaatgggaac gcatgaaacg gttgaccccg
     1561 ccgaggtcgt tcgcgaaccg tttctgggtc agttcgtaaa atgaaagcgt gttgagatac
     1621 aggtctatct cttcggcgat gcccggtatc ttggcctcct tgaactcccg gtagaggagc
     1681 gatatcttct cgagataggc gcgatcggcc atctgaccga gcaggtcggc ggtaccgagc
     1741 atcatgccga gcacccgcga ttcgaacgac cggaaccgga tgtcctggat gcggacggtg
     1801 aacccggtgc acgcgagcat gtcgcgaccg tcggccatgt ctccctcgtc gaagcccttg
     1861 gcgcgcagat aggccgagag aaaatcggcg ctccggtcga tgtggttctg tgtgtatttc
     1921 gcgcccgacc cggcatcgcc ctcctgctgg atgtagccga catcgtgcag tatcgtcgct
     1981 atgagggcaa gttcgacatt cttttcggaa acgatgttcc cctcgacgac ggcgccgtgg
     2041 atgagccgcg ccatcgcgat gaaggcatcg gtggtgtgct ggaggttgtg atattcggtg
     2101 ttgcaggggc gatagcccgg cacccgaccg tcgaacagcg cgagtatgtg ctcgaacacc
     2161 tcgcggaccc gccgggtatc aaggcccgga taggccatct gcatcgtcgt ttcgacctcg
     2221 gcacggaccg cgagtggctg atccatcgcg accagttcgg ccagttgcgg gagatgttcg
     2281 gtaccgttca taaccatgcc gccagtgtag cgaccttgtg ataacgagtc aagttttata
     2341 taactttata acaaagatac atacctttgc ataattttaa tttgcggaat cggacctccc
     2401 gcgttacact cggcgccatg ggaatcggaa ctcttcgcat cgcccgcaac ggcctggcgc
     2461 tttttatcat catcatcttt ttctctcttt gcaatgtgac cttgtgggcc gtgacgcgcg
     2521 ataccgttct tcaacgacgg cgcatccagc ggtcgacgcg cttctggata cggacgaccc
     2581 tcaacgcgct gaacatctcc tacacgctca aaggcgcgga gaacctgcgc aacgaccgca
     2641 accatctcat catcgcgaat cacatctcct atgtcgatgt cctccttctc tacgccctgc
     2701 gcccgctgac cggcgtcgcc tcggtcgagg tcgaggtcgg cggactcgac ggctttctcg
     2761 cgaagatggg cggctctctg ttcatcgagc gacgcagtgt caggcacctg cgcggcgaac
     2821 tggcgaaggt aacggcgctc ctgcgcgacc ggttcgatgt cctcatcttc cccgaaggga
     2881 ccaccaccga tggcaaaagc ccgttgaaac ccttcaggac cgccttcttc gccgccgccg
     2941 ccgatgcggg aacgccgata ctgccggtcg cgatatgcta cgaaacgatc gacggccgtc
     3001 cggcgcggga ggacgatctg ctcgacgcgg tcgcctatta cggcgacata tcgttcctct
     3061 cccacgcgaa aaaattcctc gcgctcaaaa gcatcaccgc cacgataacg gtactgccgc
     3121 cggtcgaggc cgcgtcggcg caccgcgacc aactcggcgt caccctgcgg gaacagatac
     3181 agacggcgcg ggacgccatt taatacttcc cttttcccca ctcttctgct atatacccac
     3241 cagttgtctt tattcttaag gagggaacca tgctgaccgt ccgtacggcg gcggaactcc
     3301 ggacggcgcg cgccgcgctc gccgggagcg tctgcttcgt cccgacgatg ggggcgctcc
     3361 acgccggcca cgcccggctg atggaggtgg cgaaaaagaa ggccgatcat ctcgtcgttt
     3421 ccatcttcgt caacccgacc cagttcgggc cgaacgagga cttttccaag tatccacgag
     3481 ccgaggagac cgatcttgcg ctctgcgagg cggcgggagc cgacatcgtc ttcctgccga
     3541 aggtggccga gatctaccgt accgagccga ccgtccgcgt gacggtcgac ccgaaactgt
     3601 cgggcatcct ctgcggcgct cagcggcccg gccatttcga cggcgtggcg caggtggtgg
     3661 cgatactctt caaccttgtc cggccagaca gcgcctgttt cggcgagaag gatcaccagc
     3721 aactcgccgt gatacggaaa ctggtggagg atctccattt cccggtcgcc atcgtcccgg
     3781 tcgcgacggt acgcgaaccc gatggtctcg cgctgtcgag tcgcaaccgc tatctttccc
     3841 ccgccgagcg ggccgccgcc ccggctatct accgtgccct gctctactgc aagggacgcg
     3901 ccgaacagat ccggttcgac cgcatcgccc tgccggtcgg gaaggtcgag gaggagggca
     3961 gggccctgat actcaacgac attcccggcg cgcagataga ctatttcgag atacggcaca
     4021 ccgacacgct ggaaccgacg cacttcatca tgaaccggtc gcgggccttc gcggcggtcc
     4081 gcgtcggggc cacgcggctc atagacaatc ttctgctcaa cgggtgaggc atggcgcgga
     4141 tagcacaggc ggtcacggcg caggtggcat atcccttcgc gacgctcgcc gcaaagaaac
     4201 gcgcgatgga ggtgcgcggc catacggtca tcgacctttc catcggcgac gcttcgctcc
     4261 cggcccccga cgcggcggtg acggcgctcc gtgagagatc gctcctcgcg gcgcaccacc
     4321 attactcctc ctacaacggc atcctaccgc tccgcagggc catcgcagac tggattgatc
     4381 ggcggttcgg cgtcgcgatc gaccccgaac gggaggtgac cgcgctcatg ggttccaagg
     4441 aggggctgtt ccgtttcccg caggcggtgc tcgacccagg cgacgccgcg ctccaccccg
     4501 accccggcta cccggtctac ccgctcgcga tacgggacgc cggcggccga ccggtcgcga
     4561 tggatctctc cgagaagaac gggttcctcc ccgacctcgg cgcgataccc gaaaaaacgc
     4621 gccgcgatgt ccggatgatc atcgtcaatt tcccgtcgaa cccgaccggc gcgaccgccg
     4681 gtcccgcgtt ctacggggag ctgtaccgca tggcggtgcg gtacgattgg atcgtcgtct
     4741 ccgacaacgc gtactgcgag atatacgatg gcgcgccgcc ccgcgccgcg ctcgagttcg
     4801 acccgaagca tgagcggacc gtcgagttct tctcgttctc gaagacctac agcatgaccg
     4861 gctggcggct cggctgggcg tgcggcaacg ccgatctggt acaggcgctc gtcaaggtga
     4921 aggggctgca ggactcggcc cccttcgagc cggtgcagtt cgccggcatc gcggcgcttt
     4981 ccctttccga tgccgatctc gcgccgaccc gccgcttcta cgccgataac cgcgctctca
     5041 tgaaaaaggc gctcgacgcc gcgggcatcc cctacttcga cgcacaggcc ggattctatc
     5101 tgtgggccag agtgcccgca gggctttccg ccgccggact caccgaccgt ctgcttgagg
     5161 agcgccgggt gctcgtcacc cccggcaccg ccttcggccc gaacggcgag ggctggttcc
     5221 gcattgccgc gacgcgccat cccgacgaga tgaccgaagc ggcacgccgc atctcgcagg
     5281 gacttccggc atgagatacc tcctctcgct cggctcgaac atcggcgacc gtacgacgaa
     5341 tatcgatgcg gcgctccggc tcctcgacga acggggcgtg acgatccagc gacgctcgtc
     5401 gctctaccgc accgcgccgg tcgaggcgcc tccgcaggag gatttcgtga acgcggtcgc
     5461 gctcgtcgag accccgctct cccccgaaga actgctcgac ctcaccacgg cgatcgaaac
     5521 ggctctgggc cggacgcgca ccgcgaagaa tggcccgcgc atcatcgaca tcgacatcgt
     5581 cctcagcgcg gcgggaactt accgttcgcc ccggatcgag ataccgcacc cgcgctggcg
     5641 ccagcgacga ttcgtcgtcg aaccggcccg cgagatagcg gacggcactc cgttcgctga
     5701 ccccgtcgcc gcgatagata cgcgatccct gactcaccaa cggatagaac ggatacctac
     5761 ggaggccctg ccatgaagac ccggcttgtc ggaaaattgc tcgaccggat gcctatcccc
     5821 gatgaactca aggatatcat cggcgatcag ttggaccttt cggccattat ttctcgcgaa
     5881 gtagcgacgc aactgctgga gcacggcggc aagatgaagc aggaggccaa ggccatcctc
     5941 gcgcgcgaac tggccgggtt cctcgaaaag gtggatgtcg acaaggcgct caccaacgcc
     6001 ttggagaatc tggaacttga gatacgcatc agtttccgcc gcaaggataa gaaaacggaa
     6061 ccggcgccga aaggccgggg caaagcccgt taactgatgc aacgcagtta caccatcctg
     6121 cgtgaattcc tcgccgacgc ggtgcggacg agcatgatcc tcttcaagat catgatcccc
     6181 gtctccatcg tcatcaagat actcaaagaa acggggctca tcgaactcat cggcgccgga
     6241 ttcgccccgc tgatggagct catcggcctg ccgggcgcca tgggactcgt ctgggtgacc
     6301 ggcatggtga ccaatctcta tggcggcata ctcgccttcg tggccatctc ggccgaccat
     6361 ccgctcacca cggcgcaggt gacggtactg accacgatga tgctggtcgc ccactccctg
     6421 ccggtcgaac tgcaggtggc gcgcaaggcg ggagcatcgc ttcccgcgat attagccgtg
     6481 cgggtcggcg gcggctatat catcggaacg cttctctatc tcggctaccg gctcaccgga
     6541 acgctcggtg gtccctcaac catatcgtgg gcggcgaccg cgccggccga tccgtcgctc
     6601 atggcgtggg cgctcgggga actgcgcaac tacgggatga tattcctcgt catctgcgcg
     6661 ctcctgttcc tcatggcgct actcaagaag ttcggcgtca tcgccttcat ggaccgtctt
     6721 ttcggcccgg tcctgtcgtt catcggcatc ggccgcgagg tgacgacgct caccatcatc
     6781 gggatgacgc tcggtctcgc ttacggcggc gcactcatca tccgcgaggc acagaccggc
     6841 cacatcgcgc ggcgcgacat cttctactcg atggcgctcc tcagcctcct ccacagtttc
     6901 atcgaagata ccctgctgat ggtgtcgctc ggcggccact tctcgggact gctcatcgtg
     6961 cgggctttgt tcgcgatcac ggcggtcttt gcgatggtgc ggctgacggc aaaactttcc
     7021 gatgaacggt tcgcgcggtt actgctcagt cgagcggtat cgtgagcggc cctgcctgcg
     7081 gggcgatgaa cgattctccg gtgaagcggg tgcgaccgaa acattccttg gcggaatgac
     7141 gcaccagttc ggggctgtta ttctcttccg acagatgggc gaatatcact tttttgagtt
     7201 tgtcgtgcgg cagtttttcg agcgcctcca gacactgctc gttggaaaga tgcccctgcg
     7261 cgccgcgtat ccgctgtttg aggtcgggcg gatagcgcgg gttgcggtag agcatgtcgg
     7321 gatcgtggtt cgcctcgatg atgaggtatt cgctcgccgc tatctgggcg aatatccgct
     7381 gggtcacaat gccggtgtcg gtgacgagcg taagtcgttc gccgttcggg agcgtgacgg
     7441 tgaagccgta cgggtcgcgg gcgtcgtgca tcaccgggaa cggctcgacg acgaaccggc
     7501 cgatggtcac cgggcgccgc ggcgctatct cgcgggtctc gaactcgtac cggccaccgc
     7561 tttcggcgag gtattcgatg accacagggg cggcatagac cggcaggtgc agacggtgcg
     7621 ccgtgagcgg gacgccgctc acatggtcga tatgctcgtg ggtgatgacg atgccgacga
     7681 tgcgcgccgg gtcgatcccc gccgccgcgc accggccgtt gaagcacttg aacgagagtc
     7741 cctgatcgat gagcagaagt tcgtcgccgc tctcgacgat gacggcgttc cctttcgagc
     7801 cgctcgacag tatgtggaga aggttatcgc tcaagaccga ggaacttcct gatatcgttc
     7861 acgaaggcgg ggttggtccg ttcggcgagg aatatcttga acagcgcccg catgatgcgg
     7921 tctatctctt ccatctgtat gagacggagg tgcatcgtct cgtcgtcctc gctcttttcg
     7981 ggcgccggca gtttcaccga ccgcatcgcg agcggcagcg cggtgaggat gaactgccac
     8041 tcggccttgc cgcgcgacac cttgacgcgg gccgcgaccg gtatccgccc ggtgcgcacc
     8101 gagtcgcgga tgtcatcgct cagcgccact tcctgcgtct gtatcgtttc gcggtagcct
     8161 tcgccgcgca gcgattcgag cgttatctgc tcctcgagcc gcagatctat gctgccgagc
     8221 cccagattgt cgaggaaaaa atagggatcg gacgaactgc gccagtagag ccacagcagg
     8281 aactcctgcc cgaggaacgg gaccgcctgc gccgcttcgc gttccctgcc gctcatgcgt
     8341 cgcctccgag gtcccacaga tcgccgatga tggaggtgag ttcctccggc ttgtcgatct
     8401 catcgaccgc gtcgacgatg agggagtgag cgacgcttat cgcaaaggtc ttctcgaacg
     8461 cgtcgacgaa ccgcgccacc acgcccgacg actggctgaa caggtatatc cggttctcgc
     8521 cgagattcca cgcgacctcg gcgatgacgg tgcgcggcag ggtcttcacc cggacgcggc
     8581 gcgccgccgt ttcgcgcagt tcttttttct ggtgcgccgg tatccgctcg atcttgtgct
     8641 cctccttgaa acggaactcc gcctcctcga ggaacgggcg cagttgcgaa ccgcccgacc
     8701 cgtaacggtc tatgcggagc gagaacagat agtagtcccg gaagtagcag ttcgggaact
     8761 cctcggtctc gaacgagagg agcggatcga cccagccgac cgactcgtcg cgcacatcct
     8821 ccggcgcaag cggcaggaac cggtgctcgc ggagcagttt gcgcagtttc gtcacatcgg
     8881 gcggttgttt ctcggggatg aaggtcatga aactgacgcg gccggagcgg agtttgctca
     8941 tttcgcgctc ttcgcgtcgg ggccggacag ttcggccacg ccgaccaccg ccttttctga
     9001 gatggagatg aagatggtcc cggccagttt gttcttttcg aacaggcgct tcacctcggc
     9061 gtagaggagg ttcttgagtt cctccttgtc ctcggtgtcg tattcgccga tccaggtgtt
     9121 ggcgggcagt tcggcgaagg tctcctcgcc ggtcttcacg agatggcgca ggccgacctc
     9181 ttcaagttcc ttgatgaggt tgcggacccc gggcacattc ttttgcgtga agtgggtggt
     9241 gaaggtcgca aagtaataca tcgccgctcc gttacggtcg ggcgaccata cgacaagagc
     9301 ggccccgagt caaggaaacg aactttagcg tcaaaaaacc ttactcaaca agccactggg
     9361 taaaaaataa atctttcccg ccgaccgtcg cgttcccgtc aatacctccg ttagtccacc
     9421 cgactacata aatgtcaccc gcaccatcaa ccttaatata agtaccggca tcatactctt
     9481 ccgtccccca cagcctctct atcatattgt tattaccttt tgcgtcccat ttggtgataa
     9541 aaacatcatg gcctccagag ctctcccttt cttcccagtc tccccaaata cttcccgtca
     9601 cataaacata ttctgagtca ttagcgacaa acaaagaaag agcttgggga gatagtcctt
     9661 caacatagtt tccccatcgt ttcgtccatt ctatatcgcc atctttactg atttttgtaa
     9721 ggaaaatatg gcagtcgctt atgttctcat cacaaatgtt cccagaaaca tatacatttc
     9781 ccgtcgaatc ggctagaagt gaataaatct gcatccagtc aaatgttttt gaccagagtt
     9841 tttctccgtc agaactccat ttaacaagaa tctgttctcc cccgatcctc cccgctacat
     9901 agacattatt ggttgaatcg acacttatcg cgcggccttc gttaccgccc gaagaacccc
     9961 attgtctggt ccataattta gtgccatatt tgtcccactt ggtgaggaaa atatcgccgc
    10021 aaggcaacgg ggcaccatcg ccatattctc cgcataatct accatcatgg acatttccat
    10081 cgagagatcc tcttgtcatc ccggttatat aaatgttgct tgtcgcatca agagtcaccg
    10141 cgtacgctat ctcgtcctcg tttgttcccc attctttact ccattctttg acaccggagt
    10201 cattccattt tatgagaaaa gcgtctgttc ttcctgcgca tacattatca tcaatttttc
    10261 cagttgtaga gccaaccaca taaattccgc catgaggatc tgttgccaaa gccgttgtca
    10321 tttctaattt atctgtactc caagatctgc tccatgcttt tgtcccatcc tggttccatt
    10381 tgataagagt taagctgtcg tatttgtctt cttcattatc atctatggca cctcctgcca
    10441 cataaaagtt ccctttcgca tccatcgtta aggcacttcc ggcgtcatta taaagcgttc
    10501 cccattgtct ggtaatccgc cgcatgaccc agatattttc gacacactcc gacacaacgc
    10561 ccgcctcagc tttccattca ccgttattac agatagattc ttccttaaca ccacaggcaa
    10621 tcatcagcac cattaccgca aggaacataa ggtacttaat cataagattc ttccctcatc
    10681 atcggtatcg atacgccatt gtgccggaag cgccggcatg agtcaaggaa acggtgaccg
    10741 catacctcac ccggcctccg gccgccgcga cccttccggt atcaggtaga agagcgccat
    10801 gaggagcagt atcaccggga agtggtagcg atcgccgacc accgcccacg ctgccagcgt
    10861 gaagaacggc atcagcacca ccatcagatg gatgcggacg atctgcttcg acttgaacgg
    10921 catgaccgcc ttcccccacg gatcgggatc gaggaacagc gcgaaattgc cgacgagtac
    10981 accagcaacc atcggccaga atcggtcgat gagatagacc accggcgtgt aaaagtcgga
    11041 gttgatgaac ccgttgcgcc cgaagaaggt aagcggctcc atctcggcaa agaccgacag
    11101 gaatatcccg taaaacccga aaagatatga atagacatag aacgaaacgc caccgatgac
    11161 caacgcgacg actatcatcc cggccttcag cgcaccgtcg gggacccgtt tcaattgcgt
    11221 gatccggtca cggatccgtg ttatctctcg ccccgaaaag aggatgatct gcaccggcgc
    11281 gacaacgacg caggcatagg agaagaagag acccgcaagc cacgcgctcc agcagaattc
    11341 cggcagggtc cacccgtccc gaaaggcgaa aaaggcactc agcgcaagac cggcgagcgc
    11401 cgggagatcg tgtctgaagg ggatattttt caacagagag cggcggcgct attccggcgt
    11461 ccgggcgcag cggaagccgc cggagcgatc ggtgttctcg accgggaagc cgaagcgggc
    11521 gtaggtcatg aactggacat atttgtctga ccctatcatg aacgatccgc cgcgcatcac
    11581 ccgctgggcc gggcgggtct catcgaccca cgcggtaccg tcatcgggcc gcgttccgtc
    11641 atcgtaatcg tcgtgccagt cgtcctccag ccattcccgc gcgttgcccg ccatgtcgaa
    11701 cagcccgtag gcgctgagcc ccgtctcctt gctcccgacc gggtaggaga aaccggcatc
    11761 gcacccccag tcatccggcc ccgccatcac cacattgtcg caggaggggg cg
//
LOCUS       DAOQUE010000019        11809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_6120_length_11809_cov_3.878509, whole genome shotgun sequence.
ACCESSION   DAOQUE010000019 DAOQUE010000000
VERSION     DAOQUE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11809
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6120_length_11809_cov_3.878509"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PKH10_01220"
     CDS             complement(<1..491)
                     /locus_tag="PKH10_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009303005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNT26773.1"
                     /translation="MCGVIGIFNRRPETPRRLSREDVDTLHHRGPDAQGLYNDEHVSL
                     GHRRLSILDLTEGGAQPQSTRDGRYTVTFNGEIYNYIELRRELADQGVRFYGASDTEV
                     LLAAYGVWGKRCVERFRGMFAFALYDRTARTMFMARDRCGEKPFFFYRDGECFYFASE
                     LKGL"
     gene            complement(495..1688)
                     /locus_tag="PKH10_01225"
     CDS             complement(495..1688)
                     /locus_tag="PKH10_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26774.1"
                     /translation="MPGGERLTLLPWTRDIRPWHVRSFSRARDYLREFLKRHGGGSPA
                     GDPEQDISSLARKRRAFFRKITPPLYSLTGSLFPRFFHPDKGDAVPFDPPRSAAIRIA
                     RALHGTTRKPDLIIHLYLDMYDMTEDAWARYESVCPTPWVGVRFDPPADPCRDGFCRI
                     GSCRGAFFLDEGAAQRYAAYFGDKHISFLSDVTDTRLPTSPTVFETEVRRKSAGRKIV
                     FMGGTIGGQKDLATWYELIRTASKGRFYFLQVGEVIEGTLSIEDRAAYDAVIKDPPEN
                     FFCLSGFLPDEAEFNALIRASDIIYAVYRDFRLSSNMLAKAACFEKPILVSGRYVMGE
                     RVRRYSLGLTVAEGDVDGIAEALDVLATRPGTSYGFAKYRSDFSEERFADALDSCVKH
                     CLDRG"
     gene            complement(1897..2727)
                     /locus_tag="PKH10_01230"
     CDS             complement(1897..2727)
                     /locus_tag="PKH10_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNT26775.1"
                     /translation="MIHRLLEKYPAEILSTFSAHRGSAGEELRKELDEGREERLLAPL
                     LEIFADASRDRALPERYLDLIRKATGAVLHYGQEAEDTLLEQMGMPEKGFFVDVGAHH
                     PVRFSNTYTLYRKGWRGINIDATPGSMELFRKMRPEDINIESAVSDDTAPRTFYLFED
                     AAYNTFNSELAETYVATGCPLREKREIPQRTLAGILDDHAAPGTRIDLLSIDVEGEEM
                     GVLRSNDWNRYRPDIIVIEDLAASLDDLRRSKAVAYLSHYSYEPASKMIRSLILRRKA
                     "
     gene            complement(2845..3936)
                     /locus_tag="PKH10_01235"
     CDS             complement(2845..3936)
                     /locus_tag="PKH10_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HNT26776.1"
                     /translation="MSFEVKQGEVLGIIGKNGAGKSTLLKILSRVTAPTSGCVKIKGR
                     IGSLLEVGTGFHPELTGRENIYMNGAILGMKKREIDRKLTEIIDFSGVEQFIDTPVKR
                     YSSGMYVRLAFAVAAHLDPEILVVDEVLAVGDAEFQKKCLGKMDDVSKKEGRTVLFVS
                     HNMAAVRNLCGRCLLLKHGCIAVDAPTEKVIETYMHMNDIEIKNDLGEITDRSGTRDV
                     IFSAFHIESNGKTGISSVFTGDDAVFVFTLRNRSRKKRAVDLGFSFHSSLEERLFVLY
                     SSYQDQLFAIDAGAEMIVRCLIRDLPLSPGSVWIRGRLLADGIESDYLREPVGVFSIL
                     PGDFYRTGRAMSGDIGALVMVRGEWSIDG"
     gene            3949..4110
                     /locus_tag="PKH10_01240"
     CDS             3949..4110
                     /locus_tag="PKH10_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26777.1"
                     /translation="MDDAILVGELLHFFAAAAIHRQIGVIFAPSAGPPRLQVALEGTV
                     SDSAYPILF"
     gene            complement(4145..5008)
                     /locus_tag="PKH10_01245"
     CDS             complement(4145..5008)
                     /locus_tag="PKH10_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT26778.1"
                     /translation="MMMQGSDAPDRWTTVITPVRDRRLVDIAELLVYRDLIALFIKRD
                     FITQYKQTILGPAWFLIQPLLTTAMFLVVFGKIANIPTDGVPQPLFYMGGLIVWNYFS
                     GILTQTSSVLISNVHLFSKVYFPRLVVPIAQVVAGLGRFAIQFVLFAALYVFYLVIGM
                     KSAINWTILLFPLTLSYVAILGFGVGLIISAFTTRYRDLQVGLSFLMQLWLYGSPIVY
                     PVSLVPERWQTLYSLNPIIPAMESFKHIFFGTALPPLSVFGTGIAVTLLLLVIGLFFF
                     NRVERTFIDTV"
     gene            complement(5057..5536)
                     /locus_tag="PKH10_01250"
     CDS             complement(5057..5536)
                     /locus_tag="PKH10_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT26779.1"
                     /translation="MSFKAFTLLELMITISIVAILAVAAIPMYADYTKKARTIEVPEM
                     LKEIAKAQFVFWEDPDGGRTDHYATRIGTLQWTTSLQTYNNGEAITQAVMATKSYENV
                     DGKYWIFNAHNDLTFSCGSGVVSGVGIGSAIPRDVLMVPDDWTEGACMNTTKDLHHQ"
     gene            complement(5594..7339)
                     /locus_tag="PKH10_01255"
     CDS             complement(5594..7339)
                     /locus_tag="PKH10_01255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT26780.1"
                     /translation="MTTPAVPAFLHAYRPLRTVSFAIDHLLLGKSPVGFRAMNILYHL
                     CMLSVLWMLFRRLSSSPFAALVALSFYALHPINTEVVAYISGRRDILTSLFFVTALLF
                     LVVAVERRRPLFLLGATVCGLFSFLAKEMAAMLPLVALLYLWQYEKCSGSRLLRWGVA
                     GVTLTGILGLLAITGGGSALIHGLRPSFHGGDPLTHYLTAPTLFTYYLKQSLFPLQLV
                     LDNANYPLVTSLNMKFAASLLGAGLFITVAVLLFRKGYRPASFFMAFFLVTLLPVLQI
                     VPLHEIAADHYLYLPLVGVAGLIGECAAPFEAGATTAIFSASRRVMVIALLCALVAVL
                     FAIKSLARVGEMHDIHTVLIADSRWRPVSFRGLYTMGALYLEAGFPDRAFRYYRRAWD
                     TGFRDASLYGNLINYHIVKGDHRAALLLYGEFGARSALSLSASAHVSLLHLVAGDCGR
                     ARAVAASVPDAVATRFAKKMATRCEEFRRDRFPDDPSWVDALADAGIGVELRPIWERM
                     VGDAAIPPDRRAAISERLQKTLLRFDIPAANIRFSEHLQLVSVTPGLDVDHWRREMIR
                     ARETEERIDALPDPI"
     gene            complement(7501..7998)
                     /locus_tag="PKH10_01260"
     CDS             complement(7501..7998)
                     /locus_tag="PKH10_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005297355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT26781.1"
                     /translation="MKKGFTLIELMIVIAIIGILAAVAIPMYNDYTKKARTSEVPGSL
                     KEIVKQQIAFREDPQGGNGGWADNIGSIGWKTNLNTSGAAPTNCSAGATISGATYVYA
                     CGKFFAYSSGNAGENAVGGGCGSTSDITAAASIGVGFPINSAQVPNDWVHGACMGNSL
                     SLIHY"
     gene            complement(8098..9090)
                     /gene="tilS"
                     /locus_tag="PKH10_01265"
     CDS             complement(8098..9090)
                     /gene="tilS"
                     /locus_tag="PKH10_01265"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNT26782.1"
                     /translation="MVSVPPLRWRFVRHVKKKFPQILGRKVLLAVSGGLDSMALLHLF
                     LENSSELQVTPVVAHIDHNLRKDSRRDARFVDNFCQKHGLACSVEEIPADLWKEKKGN
                     REEIARNERYRILERIAKKKRIRYVLTAHHRDDQVETVLMRILDRGSGLRGLMGISDL
                     LMRGNISYVRPLLSFSRKEIQDFMQGKRWVEDATNTDTAIRRNLFRHKVIPFLEKTLG
                     SSVGEHVAQLSDIAYGYDDVVGVALDSFWDMRRASPRSFRYLLSREEILSRSDRFWAA
                     AMAHLVRRFRGQAFGARTMKDIVGFLRGTAERADYYPIIIHNRGGKTIISIARR"
     gene            9233..10714
                     /locus_tag="PKH10_01270"
     CDS             9233..10714
                     /locus_tag="PKH10_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26783.1"
                     /translation="MCRIPPLLLKWIGIVTIGAIFSCAILFRLIFLDHIPGLNGDEAW
                     YGIQALNLMYGRPLEWMAPSGRPLLNFFFFIPIFILQLFCEPAFWILRFPSVIASIIA
                     IFLSYRLFLDILGRPAAISLALLTATLPINIAYSRFGWDPSLLIVYAVLIIYFSFRGS
                     LKGVLISTLLGLTAHPVIIFLGPVVVMTALTQNGSALLPSHPLQKKIGAVLILTCIIA
                     VAVYAAGIEATTHRLSTPQDAWLFLSLIGGLFSGETVYRYIVDPATTLAILDPASIGA
                     ILAVLSAIAFWRISSKTSQIGALAFLSGIFFALLCGYLMFGPWLLLPHTERYGIYLVF
                     PLTLLFVIILHGAFTAERQHWPVAIVMALSLSLLAGFHRHYLQPIRETGGNSQIAFRT
                     GVLDPKQVAYLGILDETRGRPVRIFAHQWWIWQPLKYLASKDPTVEVIELREFSRPIK
                     GDYVVTFAGEPLDIVARKDPSTYATRWSVLDYARREVIAVYRL"
     gene            10711..>11809
                     /locus_tag="PKH10_01275"
     CDS             10711..>11809
                     /locus_tag="PKH10_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26784.1"
                     /translation="MNKEKVFTSYCRGLKNTFGKTVITNKRSLDLARRASLTMILGIF
                     FFAIVFRLIDLDHLPGFYGDEAWYGIQAANLMHGRPIEWIALSNRPLINIFFFIPVFL
                     LQLFFEPSLWILRFPSVIAGLLAVLLSYYLFHKIIGRPAAISLALLTAALPINIAYSR
                     FGWDPSIIILYAVTIIYFALRDNLTGVLISTLLALTAHPTAICLFPIVITIALAREDS
                     TLLPTGLLQKRTGAAILLTCNMIGAIYARTLNIAAFRFPPAEEEVPFFLNLGRLFSGE
                     TTYRHIVDLSISLGATDYSILLGTLLLLSVFFFRHAGSTPDQFGKILFPLGLGLLFSL
                     LCGYLMTGPCFILPHAERYGVYLIFPCTLFFV"
BASE COUNT         2662 a         3495 c         3100 g         2552 t
ORIGIN      
        1 agccccttca attccgaagc gaaatagaaa cactctccgt cacggtagaa gaagaacggt
       61 ttttcgccgc accggtcacg cgccatgaac atcgtccgcg cggtcctgtc gtacagcgcg
      121 aaggcgaaca tgccccggaa gcgctccacg catctcttcc cccagacgcc gtaggccgcc
      181 aacagcacct cggtatcgga ggcgccataa aagcggaccc cctgatcggc gagttcccgg
      241 cgcaactcga tatagttgta tatctcgccg ttgaaggtga cggtatagcg gccgtccctc
      301 gtgctctggg gctgcgcccc cccttcggtg aggtcgagta tcgagagtct ccggtgcccc
      361 agcgagacat gctcatcgtt gtagaggccc tgcgcgtcgg ggccacggtg gtgaagggtg
      421 tcgacatctt cccgcgacag ccgccgggga gtctccggac ggcgattgaa gatgccaatc
      481 actccgcaca tcattcaacc ccggtccaga cagtgtttca cgcacgaatc gagcgcatcg
      541 gcaaacctct cttcgctgaa atcggagcgg tacttcgcaa aaccgtatga ggtgccgggc
      601 cgtgtcgcca ggacatcgag cgcctccgct atcccatcga catccccttc agcaacagtc
      661 aggcccaacg agtagcggcg gacgcgctcc cccatcacat agcggccgct aaccaatatc
      721 ggtttctcaa aacaggccgc cttggccagc atgttgctgc tgagcctgaa atctcgatac
      781 accgcataga tgatatccga ggcccgtatc agcgcgttga attcggcctc gtcgggcaaa
      841 aagccggaaa gacagaagaa attttccggc ggatccttta tcaccgcatc ataagccgcc
      901 ctatcttcaa tgctcaaggt gccttcgatg acctcgccaa cctgaaggaa atagaaacgg
      961 cctttgctgg cggtccgtat cagctcatac catgtcgcga gatccttctg tccgccgatg
     1021 gtgcctccca tgaaaacgat cttccgcccg gcgctcttgc ggcggacctc cgtttcgaac
     1081 acggtcggcg acgtgggaag tcgcgtatcg gtcacgtcgg acaggaagga gatatgcttg
     1141 tccccgaaat aagcggcgta ccgctgcgcc gccccttcgt caagaaaaaa tgcgccacgg
     1201 caggatccga tgcggcagaa cccgtcacgg caggggtcgg ccggcgggtc gaagcgcacc
     1261 ccgacccacg gtgtcgggca caccgattcg tagcgagccc acgcatcttc ggtcatgtcg
     1321 tacatgtcaa ggtacaggtg gataatgagg tcgggctttc tcgtggtgcc atgcaacgcc
     1381 cgggcgatcc gtatcgcggc ggaacgggga gggtcgaaag ggacggcatc gcctttgtca
     1441 ggatggaaga aacgggggaa cagcgatccg gtcagcgaat aaaggggggg ggttatcttc
     1501 ctaaaaaagg ccctcctctt tcttgcgagg gaagaaatat cctgctccgg gtctccggcc
     1561 ggcgacccgc cgccgtgcct tttcaggaac tcgcgcaggt agtccctcgc gcgggaaaag
     1621 gaacggacat gccacggcct gatatcgcgg gtccacggca ggagggtcag cctttcgccg
     1681 ccgggcattt caccgcggtc aacgatagcg gcattctgcg tcaaaagaag gacctcgtgg
     1741 ccccgccgca ataggttgtg ggcaaaaata ccggtccagg aatcaaggtg gccgcgatac
     1801 gaaatggggt tatataacag gacgcaccgg ctcatcggtc tgacacccga acaggtcggc
     1861 ggacaatgcc tgacagcggc acgcagcccg tacgcactat gcttttctcc tcaatatcag
     1921 ggagcggatc atctttgatg ccggctcgta agaatagtgc gacagatagg cgaccgcctt
     1981 cgaccggcgg aggtcgtcaa gcgatgccgc gaggtcctct atcacgatga tatcgggccg
     2041 gtatctgttc cagtcattgg atcgcaggac gcccatctct tccccttcca catcgatgga
     2101 aaggaggtct atccgggtcc ccggcgccgc atgatcgtcc agtatgccgg ccaaggtcct
     2161 ctgcgggatc tcccttttct ccctcagcgg gcatcccgtc gcgacatagg tctcggcgag
     2221 ttcgctgttg aatgtgttgt atgccgcatc ttcaaaaagg taaaaggtcc gcggggccgt
     2281 gtcgtccgaa acggccgatt cgatgttgat gtcctcgggc cgcatctttc taaagagctc
     2341 catggaaccg ggggtcgcgt ctatattgat cccccgccag cctttccggt agagggtata
     2401 ggtgttggag aaacggaccg gatgatgggc cccaacatcg acgaaaaatc ccttttcggg
     2461 catccccatc tgctccagga gggtatcctc cgcctcctgc ccgtagtgca gcaccgcacc
     2521 ggtggccttg cggataaggt cgaggtaccg ctcggggagg gcccgatccc gcgatgcatc
     2581 ggcgaatatc tcaagtagag gagcgagcag acgctcctcc ctgccctcgt cgagttcctt
     2641 ccggagttcc tcgccggcag atccccggtg cgcggagaat gtcgagagga tctccgcagg
     2701 atatttttcc agcagccggt ggatcacctg caagcgcgcc ctcttaaaag acaaaagaag
     2761 caaaataagt ccagacaaaa tctctcgggt cggtctatgc attggagtcc tcatcgccaa
     2821 ctacactcct tccccaaagt agcatcaacc atcgatcgac cactctcccc taaccattac
     2881 aagagcaccg atgtcgccgc tcatggccct acctgtacgg taaaaatcac cggggaggat
     2941 actgaaaaca cccaccggtt cccgaagata gtcagactct atgccgtcgg ctagcaatcg
     3001 ccccctgatc cagacagaac caggcgagag agggaggtct cgtatcaaac aacgtactat
     3061 catctctgcc ccggcatcta ttgcgaacaa ttggtcctga taggaactat ataagacaaa
     3121 gagtctctcc tccaaagagc tgtggaacga aaaaccgaga tcaaccgctc tctttttcct
     3181 tgatctattc cttaaggtaa aaacgaacac cgcatcatct ccggtgaaaa cagaggatat
     3241 ccctgtcttc ccgttacttt cgatatgaaa agcagaaaag atcacatccc ttgtccccga
     3301 cctatcggtt atttctccga gatcgttctt tatttcgatg tcattcatgt gcatgtaggt
     3361 ctctataacc ttctccgtcg gtgcatcgac agcgatgcac ccatgtttca gcaggagaca
     3421 tcttccgcac aaattcctca ccgccgccat attgtgactc acgaagagca ctgttcgtcc
     3481 ctctttcttg ctcacatcat ccattttgcc aaggcatttc ttctggaatt cggcgtcgcc
     3541 gacggccaaa acctcatcaa ccacgaggat ctcagggtca agatgggccg cgaccgcaaa
     3601 ggcaaggcgg acatacatgc cggaagaata ccgtttgact ggcgtgtcga tgaactgctc
     3661 gaccccggaa aaatctatta tctcggtgag tttccggtct atctcccgct tcttcatccc
     3721 gaggatggcg ccgttcatgt agatattctc gcgcccggtc agttcggggt ggaagccggt
     3781 accgacctcc agcaaactgc cgatacgtcc ttttatcttc acacaccccg atgtcggcgc
     3841 cgtgacgcgt gacagtatct tgaggagcgt cgatttccct gcaccgttct tgccgatgat
     3901 gccgagcact tctccctgct ttacctcgaa cgacacatcc ttcagcgcat ggatgatgcc
     3961 attctcgtcg gcgaattgct ccattttttt gcggcggccg ccatccaccg gcagattggg
     4021 gtcatctttg ccccttcggc gggcccacca cgattgcagg tcgcgctgga aggtacggtg
     4081 agtgatagcg cctatccgat attgttttga gaggttctct accgagatca ccacatcgga
     4141 catgctacac cgtatctata aaggttcttt ccacgcgatt aaaaaagaac agcccgataa
     4201 cgaggagcaa aagcgttacc gcaatccccg tgccgaagac cgacagcggt ggcagagccg
     4261 taccgaaaaa gatatgcttg aacgattcca tcgcggggat gatgggattc aatgaataca
     4321 gcgtttgcca tctttccggc acaagcgaga ccgggtagac tatgggggaa ccgtagagcc
     4381 acaattgcat gaggaacgat aaccccacct gaagatcacg atagcgggtg gtgaaagcgc
     4441 tgatgataag cccgacgccg aatccaagta tcgcgacata ggaaagcgtc agggggaaca
     4501 gaagtatcgt ccagttaatg gcgcttttca tgccgataac aagatagaaa acatacagtg
     4561 ccgcgaacag cacgaactgt atggcgaagc ggccgagacc cgccacgacc tgcgctatcg
     4621 gcacgaccaa ccggggaaaa tagaccttgg aaaagagatg gacattgctg atcaacaccg
     4681 atgaggtctg ggtaaggatg cccgaaaagt aattccagac gataagcccc cccatataga
     4741 aaagcggctg gggcacgcca tcggtcggta tattggcgat cttgccgaag accacgagga
     4801 acatggcggt ggtcaggagc ggctggatga ggaaccatgc gggaccaagt atcgtctgct
     4861 tgtattgcgt gataaaatcg cgcttgatga acagggcgat aaggtcgcga tagaccagaa
     4921 gttcagcgat atcaacaagg cgacgatccc gcaccggcgt aatgaccgtc gtccatcggt
     4981 ccggagcgtc gcttccctgc atcatcatca ggtgggactc ctctgaaaaa ggtacgcagg
     5041 gaagtatagc agggaactat tgatggtgca agtctttcgt agtgttcatg caagccccct
     5101 ccgtccagtc gtcggggacc ataagaacat ccctcgggat ggcactaccg atgccgacac
     5161 ccgagacgac accagatccg caactaaagg tcagatcgtt gtgcgcgttg aatatccagt
     5221 acttgccgtc cacattctca taactcttcg tcgccatcac cgcttgtgtg atggcttctc
     5281 cgttgttgta tgtctggagg gaggtggtcc actgcaaggt gcctatgcgg gtagcgtagt
     5341 gatcggtccg ccctccgtcg ggatcctccc agaagacgaa ctgcgccttc gctatctcct
     5401 tgagcatctc ggggacctcg atggtgcggg cttttttggt gtagtcggcg tacatcggga
     5461 tggcggcgac cgcaaggatg gcgacaatgg atatcgttat catgagttca agcagggtga
     5521 atgccttaaa agacaaccgg atcctcctac tgttatcttt cactaagcaa tcatcattcc
     5581 gccggatcat catctatatc gggtcgggga gcgcatcgat cctctcctcc gtctcgcggg
     5641 cgcgaatcat ctcgcgacgc cagtggtcga catcaagccc cggagtcacc gaaacaagct
     5701 gtaagtgttc agaaaaacgg atgttcgccg ccgggatatc gaaccgaaga agcgttttct
     5761 gaagtcgttc cgatatcgcg gcccggcgat cggggggtat cgccgcgtcg ccgaccatcc
     5821 gttcccatat cgggcgcagt tcgaccccga tcccggcatc cgcaagcgca tcgacccatg
     5881 acgggtcatc cgggaaacgg tcccggcgaa attcctcgca ccgcgtagcc atctttttgg
     5941 cgaaccgggt cgccaccgca tcagggacac tcgcggcgac ggcgcgggcc ctcccgcaat
     6001 caccggctac gagatgaagc agggaaacat gagccgaggc ggagagcgaa agggcgctcc
     6061 tcgcgccgaa ttcaccgtaa agcagaagcg ccgcgcgatg gtcacctttt acgatatggt
     6121 aattgatgag attgccgtac aacgacgcgt cgcggaaccc ggtatcccac gcccgtcggt
     6181 aatagcggaa cgcgcggtcg gggaagcccg cttcgagata gagcgccccc atggtataga
     6241 gtccccggaa cgatacgggg cgccagcggc tgtcggcgat gagcaccgtg tggatatcgt
     6301 gcatctcccc tacccgtgcc agcgacttga tcgcgaacag gaccgcaacg agcgcgcaga
     6361 gcagggcgat gaccatgaca cgccgcgagg ccgaaaagat ggcggtggtc gcaccggcct
     6421 cgaacggcgc cgcgcactcg ccgatgagac cggcgacgcc gacgagcggc aggtagagat
     6481 agtgatcggc cgctatctcg tgaagcggca cgatctggag gaccggcaga agcgtgacga
     6541 ggaaaaaagc catgaagaag gaggccgggc ggtatccctt acggaacagg aggaccgcga
     6601 ccgttatgaa caggcctgct ccgagaagcg aggccgcgaa tttcatgttc agtgaggtca
     6661 ccagcggata attggcattg tcgagaacta gttgcaacgg aaagagactc tgtttcaggt
     6721 agtaggtgaa aagcgtcggc gccgtcaggt aatgggtcag cggatcgccg ccgtgaaagg
     6781 agggtcgcag gccgtgaatg agtgcgctcc cgccgcccgt tatcgccaag agcccgagga
     6841 taccggtcag cgtcaccccc gccacacccc aacgcaaaag gcgcgatccg gagcactttt
     6901 cgtactgcca caggtagagc aacgccacga gcggtaacat tgctgccatc tccttggcca
     6961 aaaaactgaa caggccgcag accgtagcgc ccagcaggaa cagcggtcgc cgccgctcga
     7021 ccgccaccac gagaaacaag agtgccgtca cgaagaaaag ggaggtcagg atgtcgcggc
     7081 gtcccgatat gtaggcgacc acctcggtat tgatcgggtg aagcgcatag aacgacagtg
     7141 cgacgagcgc cgcgaagggc gaagaggaaa gtcgacggaa caacatccag agcacagaga
     7201 gcatgcacag gtggtagagg atgttcatcg cccggaaccc aactgggctt ttacccaaaa
     7261 gcagatggtc gatcgcgaag gagacggtcc gcaggggacg ataggcgtgc agaaacgcgg
     7321 ggaccgcggg ggtcgtcacc agatcgttct ctacggtgag cgcgcgatcg tcaaagataa
     7381 agtcgttgcg caaagaagga agatacagaa ggaccgtgac aagaaaaaca aatccaagaa
     7441 ctaaaaaaag ggggaggcgc aagcgccccc ccccagcaga aaggaacatt cgacgggaaa
     7501 ttagtagtgg atcaggctca ggctgttccc catgcacgcg ccatgcaccc aatcattggg
     7561 aacctgcgcg gaattgatcg gaaaaccgac gccgatactg gcagccgcgg tgatgtcgga
     7621 tgttgatcca caaccgccac caacggcatt ctcgcccgca ttgcccgacg aatacgcaaa
     7681 gaacttaccg caggcgtaga cataggtcgc gcctgaaata gtggcacccg ccgaacagtt
     7741 cgtcggcgca gcgccgctgg tgttcaggtt ggtcttccaa ccgatcgaac cgatgttgtc
     7801 ggcccagcca ccgttaccgc cctgcgggtc ttcacggaag gcgatctgct gtttcacgat
     7861 ctctttgagt gagccgggca cctcggaggt gcgcgccttc ttggtgtagt cgttgtacat
     7921 cgggatggcg acggccgcga ggatgccgat gatcgcgata acgatcatga gttcgatgag
     7981 cgtaaagcct ttcttcattt ggtctctcct tctttcaaac cattaataag ccacgaaaca
     8041 cgcacggtgt tttcttcggt gccataataa gcaacgagcg tgccaaaaaa aggcgcatca
     8101 tctacgggcg atcgaaataa ttgtttttcc tccacgatta tggataatta tcggataata
     8161 gtcggcccgc tcggccgtgc cgcgcaaaaa accgacgata tctttcatgg tgcgcgcccc
     8221 gaaggcctgc ccccggaatc tcctcaccag atgggccatc gcggcggccc agaaacggtc
     8281 ggaccgcgag aggatctctt cgcgggagag cagataccgg aaagatcgtg gcgatgcacg
     8341 acgcatatcc cagaaactat cgagcgccac accgacgaca tcatcgtaac catacgcgat
     8401 atcagatagt tgtgcgacat gctctcccac cgaggatccc agggtcttct caaggaacgg
     8461 tatcactttg tggcggaaca gattgcggcg gatggcggta tcggtattgg tcgcgtcctc
     8521 tacccatctt ttcccctgca tgaagtcctg tatttcctta cgcgagaaag aaagtaacgg
     8581 cctgacataa cttatatttc ctcgcatcaa gagatcggat atgcccataa gccctcgcag
     8641 gccgctcccc cggtcgagga tacgcatcag gaccgtttcc acctgatcgt cacggtgatg
     8701 ggccgtcagc acataccgga tgcgcttttt tttcgctatc ctctcaagta tccgatatct
     8761 ctcgtttctt gcaatctcct ccctgttccc ctttttttct ttccagagat ctgcgggtat
     8821 ctcctcaaca gaacacgcga gaccatgctt ctgacaaaaa ttgtcaacaa aacgcgcatc
     8881 gcgtcgggaa tcttttctca aattgtggtc tatatgggca acgaccgggg tcacctgcag
     8941 ttcggaactg ttttcgagga acagatgcaa caaggccatt gagtcgagtc cacccgaaac
     9001 tgctagaagc acttttcgac caagtatctg gggaaacttt tttttcacat gacgaacgaa
     9061 tcgccatcga agcggcggga ccgacaccat atgccctccg gtcaaaaaca tcttcccctt
     9121 gccaagaccc aacctctata ctcaaatgaa gaacaaaaca atttttcccg catttttccc
     9181 atattcgctc gtcaaaattt gacagagggc gcaccttcct tatagtgtca acatgtgtag
     9241 gatacccccc ctcctcctca aatggatcgg tatcgttacc atcggagcga tattctcctg
     9301 cgcgatactc ttccgcctta tcttccttga ccacataccc gggctgaacg gcgacgaggc
     9361 atggtacggc atacaggcgc tcaacctcat gtatggccgc ccactggaat ggatggcccc
     9421 ttccggcaga ccgctgctca acttcttctt tttcatcccc atattcatcc tgcaactttt
     9481 ctgcgaacca gcattctgga tacttcgctt cccttccgtc atcgccagta ttatcgccat
     9541 cttcctctcg tatcgcctgt ttctcgacat tctcggtcgt cctgcggcga tatcgcttgc
     9601 gctcctcacc gccacgcttc ctatcaacat cgcctactcc cgcttcggct gggatcccag
     9661 cctgcttatc gtctatgccg tgctgatcat ctatttctct tttcgcggca gtttgaaggg
     9721 ggtattgatc tcgacgctgt tgggcttgac ggcgcatcca gtgatcattt tcctcggccc
     9781 cgtcgtcgtc atgacggcgt tgacacagaa cggctccgcg ctgttgccct cccaccctct
     9841 tcagaaaaag atcggggcgg tgctcatact gacctgcatc atcgccgtgg cggtctacgc
     9901 cgcgggcatt gaagcgacca cccatcgtct ctcaacacca caggatgcgt ggctctttct
     9961 ctcgctcatc ggcggacttt tctcaggaga gaccgtgtac cgttatattg ttgatccggc
    10021 aaccactctt gccatccttg accccgcctc gataggcgca atactcgccg tcctgtcggc
    10081 gatcgccttc tggcgtattt cttcaaagac atctcagata ggggcactcg ccttcctctc
    10141 cggcatcttt ttcgcccttc tgtgtgggta tctcatgttc ggcccctggt tgctgctccc
    10201 tcacaccgaa cggtacggaa tctatctggt gttccccctc accttgcttt tcgtcatcat
    10261 cctgcacggt gcctttaccg ccgagcgaca acactggccg gtagccatcg tcatggccct
    10321 ttcgctttcc ctgctcgccg gcttccatcg ccactatctt caacccatcc gcgagaccgg
    10381 aggcaactct caaatcgcat tcagaaccgg agtactggac ccgaaacagg tggcctatct
    10441 cggcatactc gatgagacgc ggggacgccc cgttcgtatc ttcgcacacc agtggtggat
    10501 ctggcaaccg ctgaaatacc ttgcttcgaa agacccgacg gtggaggtta tcgaactgcg
    10561 ggagttcagc aggccgatca agggagatta tgtcgtcacc ttcgcggggg agccacttga
    10621 catcgtggca cgtaaggatc cctcgaccta tgcgacgcgt tggtcggtgc ttgactatgc
    10681 ccgacgagag gtcatcgccg tttaccgcct atgaacaaag aaaaagtctt cacctcttat
    10741 tgcaggggac taaaaaacac cttcgggaaa accgtcatca caaacaaacg atcccttgac
    10801 ttggcaagaa gggcaagcct tacgatgatc ttaggcatct tctttttcgc gatagttttt
    10861 cgcctgatcg atctcgacca tctccccggc ttttacggcg acgaagcatg gtatggcata
    10921 caagccgcca acctcatgca tgggcgcccc attgaatgga tcgctctctc caacagaccc
    10981 cttatcaata tctttttttt tatccccgta ttccttttgc aactcttttt tgagccatca
    11041 ctttggatac ttcgcttccc atcggtcatc gccgggcttc ttgccgtgct tctttcctac
    11101 tatctctttc ataagatcat cggtcgtccc gcggcgatct cgctcgcgct tctgaccgcc
    11161 gcgcttccca tcaatatcgc ctattccaga tttggctggg acccgagcat catcatttta
    11221 tacgcggtta caataattta ttttgccctt cgcgacaact taacgggcgt actgatatca
    11281 acattgcttg cgttaacagc gcatccgacc gccatatgtc tttttcccat cgtcatcacc
    11341 atagctctcg cgcgggaaga ttctacccta ttgcccaccg gccttttgca gaaaagaacc
    11401 ggggcggcga tacttttgac ctgcaatatg atcggagcga tctatgcaag aaccctaaac
    11461 atcgctgcct tccgcttccc tccagcggaa gaagaagtcc cgttctttct aaatctcggc
    11521 agactttttt caggcgagac cacatatcgc cacatagtcg atctctcaat ttctctagga
    11581 gcaaccgatt attccatatt gctgggaacg cttttacttt tatcagtatt cttttttcga
    11641 catgccggct ctacgccaga ccaattcgga aagatcctct tccctcttgg tctaggcctt
    11701 cttttctctc tgttgtgtgg atacctcatg acaggcccgt gttttatcct tccccacgct
    11761 gaacgatatg gtgtatatct cattttcccc tgtaccctgt tctttgttg
//
LOCUS       DAOQUE010000020        11744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_6161_length_11744_cov_4.529985, whole genome shotgun sequence.
ACCESSION   DAOQUE010000020 DAOQUE010000000
VERSION     DAOQUE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6161_length_11744_cov_4.529985"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PKH10_01280"
     CDS             complement(<1..442)
                     /locus_tag="PKH10_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNT26785.1"
                     /translation="MKVVIAGAGTVGFETARQLIEEKRDVVLIEHSLVRAKHASSHLD
                     CMVITDEATNPDALRKAGGEDISHFIAVTDSDEVNLVTCGMARGIFPASRTIARVRNL
                     HYAKATYHGEPFLGVDYVVTPEVEAARQILDTILHGAVSDVLRFE"
     gene            657..2018
                     /gene="gdhA"
                     /locus_tag="PKH10_01285"
     CDS             657..2018
                     /gene="gdhA"
                     /locus_tag="PKH10_01285"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNT26786.1"
                     /translation="MSYMKDLIAKTKAKNSNEPEFIQAVEEVMLSLEPTVKKHPEFVA
                     ANIYERIVEPDRQIIFRVPWVDDEGNVHVNRGFRIEFNMAIGPYKGGLRFHPSVNLGI
                     IKFLGFEQIFKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFTQSFMRELFRYIGPDTD
                     VPAGDIGVGGREIGYMFGYYKKIVNEHTGVLTGKGLSYGGSLIRPEATGYGSVYFAAE
                     MLATRKQELKGKTCIVSGSGNVTQYTIEKINQLGGKAISASDSNGTIIDMDGIKGDRW
                     EFLMDLKNNRRGRIKEYAAKYPNAKYYDGKSVWDVVKEQGIKVECAFPSATQNEISKD
                     HAVALVKNGCYCVSEGANMPSVPEAIEVYLENNVLYGPAKAANAGGVATSGLEMSQNS
                     MRLSWSREEVDAKLHDIMKRIHASCRDAATTYGFKPDNYAAGANIAGFVKVANAMLAA
                     GIV"
     gene            2129..2593
                     /locus_tag="PKH10_01290"
     CDS             2129..2593
                     /locus_tag="PKH10_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNT26787.1"
                     /translation="MDSLFGIALNSWSFWMVTAVVLFIIEIFTPGFFIATFGVGALCT
                     VIAALITDSMNFQLLVFAVSTTLTYVFVRPFAKRYFFKKTPDLPTNADALVGKKAKVI
                     ETVDNANSKGRVKLFGGEDWKAVSVDDQIIERDIVVEITSVDGAKVTVKKIS"
     gene            2614..3600
                     /locus_tag="PKH10_01295"
     CDS             2614..3600
                     /locus_tag="PKH10_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNT26788.1"
                     /translation="MGTIGFILTVIVVLVIVIGTKGIRIVPQAHTMIIERLGRYHETL
                     VSGINFIIPIIDKPRDMDWKASRKTFDGRSIIENRKITLIDLREAVYDFPRQNVITRD
                     NVVIEIDAIIYYQITNPMKAVYEIQNLPDAIEKLTKTTLRNIIGEMDLDETLSSRDKI
                     NHKLRDILDDATDKWGVKVNRVEIQDIQPPHDIREAMEKQMRAERDRRAIILNAEGDK
                     SARILESEGYRQKEINQADGDKQARILQAEGEAQAKIRIAEAEAQALKLVADALKATK
                     MDAGSYLVALKYIEAFRNISEGEKDKVIFMPYEATGVLSSIGSIKEIFNVQK"
     gene            complement(3621..4790)
                     /locus_tag="PKH10_01300"
     CDS             complement(3621..4790)
                     /locus_tag="PKH10_01300"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT26789.1"
                     /translation="MTEIETLKKRIAELEDENLRLKAGAGMTFLAGSVVRTDTKTSNM
                     TVAGIEEMVLLISRDNSLSYVNGPMGKLLGMTDRKSVLGRPLAEWDRLPGAEGTLLSL
                     VNMAREGDGALTVEPPLNHFPVERLPDPAAARPAGPIFLRITAFRTGDKVQISIQETT
                     RLKWLEKTFSRYLPKKIIEQLQFVSDDEVMRPQRANVTMLFADLRGFTAISERISPED
                     VSRIVSSFLGEMVAVINEMDGTVDKFVGDEIVAMFGAPQKQPDHAVRALIAATRMMER
                     HTRWMATQTAKGEPAPQAGIGIATGDVVIGNIGTEDRSDFTAIGHAVNLASRLCGSAP
                     GGMIYTVPETYAAASAAMKLYLGPIPLPKIGFDPVGDLTFKNVSKPVKVLSVRVG"
     gene            complement(4817..9160)
                     /locus_tag="PKH10_01305"
     CDS             complement(4817..9160)
                     /locus_tag="PKH10_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNT26790.1"
                     /translation="MKHDLLFSILAAQNGLIKADELVEIGSAWAANPAKGLKERIGER
                     VSADDMELLERLVVRALEKHGGDIERSIRALNADELLFKTLGGAVKLEEPKEEEGCAC
                     GDPICEKITLESPGRYTIKEEKNRGGIGKIFLAFDEHIGREIAVKELLCEGGGTGSQK
                     DSEGKTPSRPSKALKARFLREARVTGQLEHPSIMTVYEIGQRLDGTCYYTMPFVRGIT
                     LLQAIRANPEPADRLNLLPRFHDLCNAMAYAHSKGVVHRDIKSENVMIGEFGETVVLD
                     WGLAKIHGQSDIGAEKLEKGLELLRDAEAGKTVAGHAMGTPAYMPPEQAMGEIEEIDE
                     KSDIYSLGAVLYEILTGQPPHTGLTAFDVIAKVLNDPIVRADERDKRIPRDLAAIAEK
                     ALSRRKEDRYVSAVAMNRDVEAYLTGGKVGAYEYSRWEIVKLIARKHKPLVVAVGAII
                     GVLLVATTVIYRNYTYALRQEAMAERSLKEAEASEAESQRNLADAYLDRAGNFLAARD
                     YLSAEMYAALSLHVNPENPYSPNCTACDKYLVDDPGESLPDSIVNAQSILYHASMHGQ
                     VSYMGMFAKGGDAVLKAAFSPDGKLLATAHPLMIRLWDARERKLIREYSAYGWVWSLA
                     FSPDGKTLAAGSYGTTVQTYDVTDKSPYVSRNLMAATKTATLGKLGNVFSLDFSPDGK
                     YLAVGCARGEVALLDPHSLISQKMIRVSKGSILAVQFAKNGTELRFATSQGTMAAMSV
                     PGLNEIDNATLLENGKDAHFRAMATDPESDDTFLATGAWIYRYRRGAQPSFERFLRID
                     APNQAPSALRLLPGGLLTVGAMDGRIRFYDVAWKRILQEIMAHEQFIADIAVAPKGSV
                     IVSVSQEDLSAKAFNITALIRKQTGAVRRTTRKDEGFGKSIWASDWHYRAECDDNRID
                     IYKAGKPESLWATFRKGKETNCERFSFPSDPHYFVATNGKEQAITLYDLVAKKEMKTH
                     FPIPPFPQKEGPYCWDISKDQKRLAIASHAYHHLAVFDIDSGALLFDDAATFKNHIFS
                     VAFGPNANEIAASERRIVLFDISGKRKIREMGFSSDYIYEINISPDGETLVAVQEKVL
                     KAYQYATGKPYFSSQGNSPFMARLSSDSTMFLSVHNDLSVKFWTVKGFQRQTFQPSDY
                     YEEVGFSGYGKEFYVEYANEIVAYPMNFNLWRKRSAELLYDVSRRSGTYPSEKGMVAI
                     KRQRVAILYGFENDDQNGARLVQTVPGAVAENIYLQPLDAPLFAPNSAIGENDAMEEV
                     IRHTTFEYVLFVSKTDAADGTQKVLFRLYERCSGSMLVQGEATVPAEGTKDRYKALTD
                     AIAVEMQKAFSPEQVGSRLSSALPKTIMRECYERALQFEPELAGKLNLSFTTLPDGRM
                     AKINYTEGSDIRSPRLLRCLEKRLSQYQFPPTGEESQWAVPLIFSPYDPSSHEVDGVK
                     NLFRVRTEPKH"
     gene            complement(9157..11556)
                     /locus_tag="PKH10_01310"
     CDS             complement(9157..11556)
                     /locus_tag="PKH10_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNT26791.1"
                     /translation="MAEKALNKRKEDRYPSALAIASDIESYMAGGRVGAYEYSSWELA
                     KKFVARNKMVSALIVALVASLALGMIVSMNARAKEKEARVSAELAQAKAEDNEKQVHY
                     QLSLSFTEKAQRSYDEKDFLSSRIFAAAALVHSPYNPYGPYRFENIEQRSDRNTKLNL
                     VEAHSALFQASKGGHIDNPVSFQRHDKFVWAVAFSHDGKMLASGGADKTIKLWNIKEG
                     KMLATFSGHESGVLSVAFSPDNKMLVSSSGDKSIRLWDVKEQKMVGSLIGHDRNAHSV
                     SFFSDGKMLASGSSDNTIKLWNVNKKELVSTFYGHNDGVASVAVSPDNKTIASASWDK
                     TIKIWSVEKHSILATLNGHNDRVNSVVFSPDGKMLASGSRDNTIKIWDMQNKGLVATL
                     EGHKDRVTSLMFSPNGKTLVSGSWDKTVKLWDIATKKIMETFAGNKSAVWSVAFSPDG
                     DSVVSGNEEGIVIKWDIVLRKNIKSLTGHSAEVFIAAFSPDGRLLASGSDDKTVKIWD
                     IEGGKIISTLTGHSNAIIALAFSSNGKLLASSGLDHHIKIWDLEKKESISDFVGHGNW
                     VRSLAFSPKGDLLASASDDRTIKIWDMSSRNLLDTIIAHDAFVYFVAFSPKGEMIASA
                     GDNAVKIWDVKTKKMIRELLGHQDRVSSVTFSSDGKLLASSSKDRTIKLWEVISGRLL
                     RTMEGHREWANYVKFSPAGDSLLSSSMDDTVGIWNVGAGECLQMLRLSWGGNEPSFHP
                     DGKSFAVANDNDVMIFPIDLDHWKRDPRDLLREAERSAGMKLDGFTLRPMTPEEFEAY
                     Q"
BASE COUNT         2621 a         3387 c         3023 g         2713 t
ORIGIN      
        1 cctcgaaccg caggacatcg ctcactgcgc catgcaggat ggtatcgagt atctggcgtg
       61 ccgcctcgac ctcgggggtg accacgtagt cgaccccgag gaacggctcg ccgtgatagg
      121 tcgccttcgc atagtggagg ttgcggaccc gggcgatggt ccgcgaagcg gggaagatgc
      181 cgcgcgccat gccgcaggtc acgagattca cctcatccga atcggtcacg gcgatgaaat
      241 gggatatatc ttcgcccccc gccttacgca aagcatccgg gttggtcgcc tcgtcggtga
      301 tgaccatgca gtcgaggtga gaggaggcgt gcttcgcgcg aacgaggctg tgctcgatga
      361 gcaccacatc acgtttttct tcgataagtt ggcgcgccgt ctcgaatccg acggtgcccg
      421 cacccgctat gaccactttc atggcgccta ctgtatcccg cgccgttctc aagtcaagtt
      481 caccgaaaag gtctttggcg accgcgcttt ctgttagagc gccgttagcg cggaggcata
      541 tactggcgac ttctttagga ttgcgatagt cgcaattgct tgacttgact atgtttttag
      601 agcggtgcta tatgtcgctc gtggcccgtt attaaccttc ttttgtgagg atccctatgt
      661 cgtacatgaa agaccttatc gccaaaacca aggcaaagaa ttcaaacgaa cccgaattca
      721 ttcaagcggt cgaagaggtg atgctctccc ttgagccgac ggtcaagaag catcccgaat
      781 ttgtcgccgc caacatctac gagcgcatcg tggaacccga tcgtcagatc atcttccgcg
      841 tcccgtgggt ggatgacgaa ggcaatgtcc atgtcaatcg cggcttccgc atcgagttca
      901 acatggcgat cggcccctac aagggcggtc tccggttcca tccctcggtc aacctcggca
      961 tcatcaagtt cctcggcttc gagcagatct tcaagaactc gctgaccacc ctcccgatgg
     1021 gcggcggcaa gggcggctct gatttcgacc cgaaaggcaa atcggataat gaagtgatgc
     1081 gcttcaccca gtcgttcatg cgcgaactgt tccgctacat cggtcccgac accgatgtcc
     1141 cggcgggcga tatcggcgtg ggcggccgcg agatcgggta catgttcggc tactacaaga
     1201 agatcgtcaa cgagcacacc ggcgtgctga cgggcaaggg cctttcctac ggcggctcgc
     1261 tcatccgtcc cgaggcgacc ggctacggct cggtctattt cgcggccgag atgctcgcga
     1321 cccgcaagca ggaactcaag ggcaagacct gcatcgtgtc gggctcgggc aatgtcactc
     1381 aatacaccat cgagaagatc aaccaactcg gcggcaaggc gatatcggcc agcgactcga
     1441 acggcacgat catcgacatg gacggcatca agggcgaccg ctgggagttc ctcatggatc
     1501 tcaagaacaa ccgtcgcggc cgcatcaagg agtacgccgc caagtatccc aacgcgaagt
     1561 attacgatgg caagtcggtg tgggatgtcg tgaaggaaca gggcatcaag gtcgaatgcg
     1621 cgttcccgtc ggcgacccag aacgagatca gcaaggacca cgccgtcgcg cttgtgaaga
     1681 acggctgtta ctgcgtatcc gaaggcgcca atatgccgtc ggtgccggaa gcgatcgaag
     1741 tctatctgga aaacaatgtc ctctacggac ccgccaaggc ggccaatgcg ggcggcgttg
     1801 ccacctccgg cctcgagatg tcgcagaaca gcatgcgcct ttcgtggagc cgcgaagagg
     1861 tcgatgcgaa actgcacgac atcatgaagc gcatacacgc ctcctgccgt gacgcggcga
     1921 cgacctacgg tttcaagccc gataactacg cggcgggcgc caacatcgcc gggttcgtca
     1981 aggtcgccaa cgcgatgctc gcggccggta tcgtctagta aaacatctcc ctaacattcg
     2041 cctccccctc aaaaaattgc tttacttctc catttcattt accctgatat ccgcagaagg
     2101 tatcctcatc aacgcaggga ggcgtcgcat ggacagtctg ttcggtatcg cactcaattc
     2161 atggtcgttc tggatggtga cggcggtcgt gctcttcatc atcgagatat tcacccccgg
     2221 gttcttcatc gccaccttcg gcgtgggggc attgtgtacc gtcatcgcgg cgctcatcac
     2281 cgacagcatg aactttcaac tgttggtgtt cgccgtttca accacgctca cctatgtgtt
     2341 cgtccgtccc ttcgcgaaga gatatttctt caagaagacc ccggacctgc cgaccaacgc
     2401 cgatgctttg gtaggtaaaa aggcgaaggt catcgaaacg gtggataatg ccaattcgaa
     2461 gggccgcgtc aaactgttcg gcggcgagga ctggaaggcg gtaagcgtcg acgatcagat
     2521 catcgaacgg gatatcgtcg tcgagataac ctcggtcgac ggcgcaaagg taaccgtcaa
     2581 aaaaatatcg tagcaacacc aagggaggca tccatgggaa ccatcggttt catccttacc
     2641 gtcatcgtcg ttctcgtcat cgtcatcggc acgaaaggca ttcgcatcgt accgcaggcc
     2701 cacaccatga tcatcgagcg gctcggtcgt tatcacgaaa cgctcgtgag cggcatcaac
     2761 ttcatcatcc ccatcatcga caagccgcgc gacatggact ggaaggcctc tcgcaagacc
     2821 ttcgacggcc gctcgataat cgagaaccgc aagataacgc tcattgacct gcgcgaggcg
     2881 gtgtacgatt tcccgcgcca gaatgtcatc acgcgcgaca atgtcgtcat tgagatcgac
     2941 gccatcatct actatcagat aaccaacccg atgaaggcgg tctacgagat acagaacctg
     3001 cccgacgcca tcgagaaact caccaagacc acgctccgta atatcatcgg cgagatggat
     3061 ctcgacgaga cgctttcgag ccgtgacaag ataaaccaca aactgcgcga catcctcgac
     3121 gacgcgaccg acaagtgggg cgtcaaggtc aaccgcgtcg agatacagga catccaaccg
     3181 ccgcacgaca tccgcgaagc gatggaaaaa cagatgcgtg ccgagcgcga ccgccgcgcc
     3241 atcatcctga acgccgaggg cgacaaatcg gcgcgcatac tcgaatcaga gggatatcgc
     3301 cagaaagaga taaaccaggc cgacggcgac aagcaggcgc gcatcctgca ggcggagggc
     3361 gaggcgcagg cgaagatccg catcgccgag gccgaggcgc aggctctgaa actcgtcgcc
     3421 gacgcgctca aggcgaccaa gatggatgcc ggcagctacc tcgtcgccct caaatacatc
     3481 gaggccttcc gcaacatctc ggaaggcgag aaggacaagg tcatcttcat gccctacgag
     3541 gcgaccggcg tcctttccag catcggctcc atcaaagaga tattcaacgt tcagaagtaa
     3601 gactgaaccg gacgatcccc ctaccccacc cgcaccgaaa gcaccttcac cggctttgag
     3661 acattcttga aggtcaggtc accgaccggg tcgaacccga tcttgggcag aggtatcggc
     3721 cccagataga gtttcatcgc agccgacgcg gcggcatatg tctcgggcac ggtatatatc
     3781 atcccgccgg gagcgctgcc gcagaggcgg gatgcaaggt tgaccgcgtg cccgatggcg
     3841 gtgaagtcgc tccggtcctc ggtgccgatg ttgccgatga cgacatcgcc ggtcgcgatg
     3901 ccgatcccgg cctgcggcgc gggctctcct ttcgctgtct gcgtcgccat ccaccgggta
     3961 tggcgctcca tcatgcgggt cgccgcgatg agagcgcgga ccgcgtggtc gggctgtttc
     4021 tgcggcgcac cgaacatcgc cacgatctcg tcaccgacga acttatcgac cgttccatcc
     4081 atctcattga tgaccgcgac catctcgccg aggaaggaac tcacgatgcg cgaaacatcc
     4141 tcgggcgata tccgttcgct gatggccgtg aacccacgca ggtcagcgaa gagcatggtg
     4201 acattggccc gttgagggcg catgacctca tcatccgaca caaattgaag ttgctcgatg
     4261 atctttttgg gcagatagcg agaaaatgtc ttttccagcc acttgagtcg ggtcgtttcc
     4321 tgaatggata tctgcacttt gtctccggtc ctaaaagcag tgatgcgcag gaatatcggc
     4381 ccggcgggtc gtgctgcggc cgggtcgggc aggcgttcca ccgggaaatg gttcagtggc
     4441 ggttcaacgg tcagtgcccc atccccttcg cgcgccatgt tcacgagcga caagagcgtt
     4501 ccctcggccc ccgggagccg gtcccactcg gcaagcggcc tgccgagcac cgacttgcgg
     4561 tcggtcatgc cgagcagttt gcccatcggc ccgttcacat aggacagcga attgtcacgg
     4621 gagatgagca gcaccatctc ttcgatgccg gcgacggtca tgttcgaggt cttcgtgtcg
     4681 gtcctgacca cgctgccggc aaggaaggtc atcccggcac ccgccttcag gcgcagattc
     4741 tcatcctcga gttcggcgat ccgtttcttc agagtctcga tctcggtcat gggacgctcc
     4801 ttttatgctc gatacctcaa tgcttcggct ccgtgcgtac tctaaagaga tttttgacgc
     4861 cgtcaacctc gtgcgaggat gggtcgtacg gggagaagat caacggcacg gcccactgcg
     4921 attcctcccc ggtcggcggg aactggtatt gtgaaaggcg cttctccaga cagcgaagga
     4981 ggcgcggcga cctgatatcg gaaccttccg tgtagtttat cttcgccatg cgaccatcgg
     5041 gcagggtggt gaaagagaga ttgagttttc cggcaagttc cggctcgaac tggagcgccc
     5101 gctcatagca ttctcgcatg atggtcttcg gaagggccga agaaagccgg gacccaacct
     5161 gttcgggaga gaacgccttt tgcatctcaa cggctatcgc atcggtcaac gccttatagc
     5221 ggtccttggt cccctccgcg ggcaccgtgg cctcgccttg caccagcatg gagccggaac
     5281 agcgctcgta gagccggaaa aggacctttt gtgtgccatc ggcggcgtcg gtcttcgata
     5341 cgaagagcac atattcaaaa gtggtgtgcc ttatcacttc ttccatcgca tcattttcgc
     5401 cgatggcgct attgggggca aaaagaggag catcgagcgg ctgcaaatag atattttcag
     5461 cgacagcccc gggtaccgtc tgaactagtc tagcgccgtt ctggtcatca ttttcaaaac
     5521 cgtacagtat ggctacgcgt tgccttttta tcgcgaccat tcccttctca gaaggatatg
     5581 ttccggaacg acgagaaaca tcgtatagca attctgcgct tctcttcctc cagagattga
     5641 aattcatcgg ataggcaacg atctcgttgg catactccac atagaattct tttccatagc
     5701 cggaaaaacc aacttcctca taatagtcag agggctgaaa ggtctgtctt tgaaacccct
     5761 tgaccgtcca gaatttcaca gaaagatcgt tatggacgct caagaacatg gtggaatcgg
     5821 acgaaagacg ggccatgaaa ggagagttcc cttgtgagga aaaatatggt ttgccggtgg
     5881 catactggta tgccttgagc accttttctt gcacagccac aagggtctcc ccgtctggtg
     5941 agatgtttat ttcgtatatg tagtctgagg aaaatcccat ttctcttatt tttcttttcc
     6001 ccgatatgtc gaaaagaact atacgacgct cactggcagc tatttcgtta gcattaggac
     6061 caaaggcaac ggaaaatatg tgattcttga aggtggcagc atcatcgaac agcaatgcac
     6121 cgctgtcgat atcgaagacc gctaggtgat gatacgcatg gctggctatt gccagtcgct
     6181 tttgatcttt agagatatcc caacaataag gaccttcttt ttgagggaaa ggaggaatag
     6241 gaaaatgggt tttcatctct tttttagcga caagatcgta aagagtaatg gcttgttctt
     6301 tgccgttagt tgcaacaaaa taatgcggat cacttggaaa cgaaaaacgc tcgcaattgg
     6361 tttctttccc ctttctaaat gttgcccata atgattccgg tttccctgct ttataaatgt
     6421 cgatccggtt atcatcacac tctgctcgat agtgccaatc cgaagcccaa atagatttgc
     6481 cgaagccctc atcttttctt gttgttcgcc tcacggcccc tgtctgctta cgaataagcg
     6541 cggtgatgtt gaacgccttc gccgaaaggt cctcttggct taccgaaaca atgacggaac
     6601 ctttcggagc cactgcgata tcggcaatga actgttcgtg cgccatgatc tcttgcaaga
     6661 tccttttcca tgcaacatca tagaaacgga tcctgccatc catggccccg acggtcaaca
     6721 gccccccggg aagaagacgc agggccgatg gggcctgatt gggggcatca atacgcagga
     6781 accgttcaaa ggaaggctgc gcgcctcgcc ggtatctgta tatccaagct ccggtagcaa
     6841 ggaaggtgtc gtcactctcg ggatccgtcg ccattgcacg gaaatgcgca tccttcccat
     6901 tctccaataa tgtggcatta tcgatctcgt taagaccagg caccgacata gcagccatgg
     6961 tgccctgtga ggtcgcgaac cgcagttctg tgccattctt agcaaattgt accgcaagaa
     7021 tgctcccttt acttaccctg atcatttttt gcgatatcaa cgaatgcgga tccaacagag
     7081 cgacctctcc cctagcgcac ccgacggcaa gatattttcc gtcaggagaa aaatcgaggg
     7141 aaaaaacatt cccgagttta ccgagcgtcg cggtctttgt tgccgccata agatttcgag
     7201 agacatatgg tgatttatcg gtcacatcat atgtctggac cgttgtgccg taacttccag
     7261 cggcaagcgt tttaccgtca ggggaaaaag caagcgacca gacccagccg tatgccgaat
     7321 attctcttat cagtttccgt tcccgcgcat cccaaagacg gatcatgagg gggtgcgcgg
     7381 tcgcgagcag tttaccatcg ggagagaagg ccgccttcag aacggcatcg cctcccttcg
     7441 cgaacatccc catataggag acctgaccgt gcattgatgc gtggtagaga attgattgtg
     7501 cgttaacgat ggaatcgggc aaggactcgc ccgggtcgtc caccagatac ttgtcacacg
     7561 cggtgcaatt gggactgtag gggttctcgg ggttgacatg gagcgagagc gcggcgtaca
     7621 tctcggccga aagatagtcg cgcgccgcaa gaaagttccc ggccctgtcg agataggcgt
     7681 cggcgagatt cctctgcgat tctgcctcgc tcgcttcggc ctccttaagg cttctttctg
     7741 ccatcgcctc ctgtcggagc gcataggtat agttgcggta gatgaccgtc gtcgcgacga
     7801 gcagcacccc tatgatggcg ccgacggcga cgaccagcgg cttatgcttc cgcgcaatga
     7861 gtttcactat ctcccagcgc gaatactcgt aggcgcccac cttgccgccg gtgaggtacg
     7921 cctcgacatc gcggttcatc gccacggccg agacatagcg atcctccttg cgacggctca
     7981 acgccttttc cgctatcgcc gcaaggtcac ggggaatgcg tttgtcgcgc tcgtcggcgc
     8041 gtacgatggg gtcgttgagc accttcgcga tgacatcgaa cgccgtgagc cccgtatgcg
     8101 gcggctgacc cgtcagtatc tcgtacagca ccgcgccgag cgagtagatg tcgctcttct
     8161 cgtctatctc ctctatctcc cccatcgcct gttccggcgg catgtaggcg ggggtcccca
     8221 tcgcgtgccc cgccactgtt ttgcccgcct cggcgtctct gagaagctcc agaccctttt
     8281 ccagtttctc ggcgccgatg tcgctctgcc cgtgtatctt ggcaagcccc cagtcgagca
     8341 ccaccgtctc accgaactcg ccgatcatca cattctcgga cttgatgtcg cggtgaacga
     8401 cccccttgct gtgagcatag gccatggcgt tgcacagatc gtggaaccgc ggcaacaggt
     8461 tcagtcggtc ggccggctca gggttcgccc gtatcgcctg caacagggtg atgccgcgca
     8521 cgaagggcat ggtgtagtaa caggtgccgt caagccgttg gcctatctcg tacaccgtca
     8581 tgatggacgg gtgttccaac tggccggtca cccgcgcctc gcgcaggaac cgggccttga
     8641 gcgccttcga ggggcgcgac ggcgtctttc cctcgctgtc cttctgggat ccggtgcctc
     8701 ccccttcgca caacagttcc tttaccgcga tctcccggcc gatatgttcg tcgaaggcga
     8761 ggaatatctt tccgataccg ccccggttct tttcttcctt gatggtgtag cgaccggggc
     8821 tttccagcgt tatcttttcg cagatcgggt cgccgcaggc gcatccttct tcttccttcg
     8881 gctcctccag tttcaccgcg ccgccgaggg tcttgaacag cagttcgtcg gcgttcaacg
     8941 cccggatgga tcgctcgatg tcgccgccgt gtttttccag cgctcgcacg acgagccgtt
     9001 ccagcaactc catgtcatcg gcgctcaccc gttcgccgat gcgctccttg agtccctttg
     9061 cggggttcgc cgcccacgcg gagccgatct cgaccagttc gtccgccttt attaggccat
     9121 tttgcgccgc gaggatggag aagagcaggt cgtgtttcat tggtatgcct cgaattcttc
     9181 cggggtcatc ggacgaaggg tgaagccgtc gagtttcata ccggccgacc tttcagcttc
     9241 acggagaaga tcccgcggat cgcgtttcca gtggtcgagg tcgatgggaa aaatcatcac
     9301 atcattgtcg ttggcaacgg caaaagattt tccatcagga tggaaagaag gctcatttcc
     9361 accccaagaa aggcgaagca tttgaagaca ctctccggca ccaacattcc aaattcccac
     9421 agtgtcatcc atgctactcg aaagcaaaga atcaccggcg ggagaaaact tgacataatt
     9481 ggcccactcc cgatgcccct ccatggttct caacaatcta cccgatatta cttcccataa
     9541 ttttatcgtc ctgtctttgc ttgaagatgc cagcaatttt ccatcagaag aaaaagttac
     9601 gctgctaaca cgatcttgat gtcccagcag ttctctgatc attttctttg tttttacatc
     9661 ccagattttt actgcattat ccccagcaga tgctatcatt tctccctttg gtgaaaatgc
     9721 aacaaaatac acgaaagcat cgtgcgcaat tatcgtatct agaagattcc ttgaagacat
     9781 gtcccatatc ttaatcgttc tatcatcact tgctgatgcc aaaagatccc ccttgggaga
     9841 gaatgccaaa gaccttaccc aattgccatg gccaacaaag tcgcttatac tttctttctt
     9901 ttcgaggtcc catattttaa tatgatggtc taacccgcta gaagccagca gttttccgtt
     9961 agatgaaaac gccagggcaa tgatggcatt cgaatggcca gtgagggtcg aaataatctt
    10021 ccctccttca atatcccata ttttcacagt tttgtcatcg cttcctgaag caagaagcct
    10081 tccgtcaggg gaaaaagcag cgataaacac ttcagccgag tgtccggtga gcgatttgat
    10141 attctttctt aaaacaatgt cccatttgat aacaatgcct tcttcgtttc ctgaaaccac
    10201 agagtcacca tcgggagaga aagcaaccga ccaaacggca gatttgtttc cagcaaaggt
    10261 ttccatgatt ttttttgtcg caatatccca aagtttaacg gtcttatccc aacttcccga
    10321 caccaatgtt ttgccgtttg gagaaaacat taaggaggta accctatctt tgtgtccttc
    10381 gagtgtagca acaagtccct tgttctgcat gtcccatatt tttatggtgt tatcgcgact
    10441 tcccgatgcc aacatctttc catctggcga aaaaacgaca ctattaactc tgtcgttatg
    10501 tccattaagc gtggcaagta tagaatgctt ctcaacactc caaatcttga ttgttttatc
    10561 ccaactcgca gaagctattg ttttattatc aggtgaaacc gctaccgaag caactccatc
    10621 gttatgccca taaaatgttg ataccagttc tttcttattt acattccata gttttattgt
    10681 gttgtcggaa ctgcccgatg ccaacatctt gccatcagag aagaaagata ccgaatgagc
    10741 gtttctatca tgccctataa gggaacctac cattttttgt tctttgacat cccacaatct
    10801 tatgctttta tcgccacttg atgaaaccaa cattttatta tctggagaaa aagcaactga
    10861 aagaacccct gattcatggc ctgaaaatgt ggcaagcatt ttaccttctt ttatgttcca
    10921 taactttatt gttttatcag cacctcctga tgctaacatt tttccatcat gcgaaaaagc
    10981 aacagcccat acaaatttgt catgtctttg aaacgaaacg gggttatcaa tatggccacc
    11041 tttgcttgct tgaaacaacg cagagtgcgc ctcgacaaga ttcagcttgg tgttcctgtc
    11101 ggacctttgc tcgatatttt caaaccggta cgggccatac ggattgtagg gactatgcac
    11161 cagcgcggcg gcggcaaaga tccgggaact tagaaaatct ttttcatcat atgaccgttg
    11221 agctttttcc gtgaatgaca aagacaactg atagtgcacc tgcttttcat tatcttctgc
    11281 cttcgcttgc gccaactctg cacttacgcg agcctccttt tcttttgctc gtgcattcat
    11341 cgaaacaatc atgccgagcg cgagcgatgc caccaacgca acgatgagtg cgctgaccat
    11401 cttgttccgc gccacgaatt tcttcgccaa ttcccacgaa ctgtactcgt acgcgccaac
    11461 ccgacccccg gccatgtaag attcgatgtc ggaggcaatg gcgagcgccg atggatagcg
    11521 gtcttctttt ctcttgttca gcgccttttc cgccaccgcc cccaactcct tcggtatccg
    11581 cctatcgcgt tgaaccgtcg gcacaatggg atcgttgagc accttggcga tgacatcgaa
    11641 cgccgtgagc ccggtgtgcg gcggctgacc cgtcagtatc tcgtacagta ccgcgccgag
    11701 cgagtagatg tcgctcttct cgtctatctc ctctatctcc ccca
//
LOCUS       DAOQUE010000021        11555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_6298_length_11555_cov_6.815826, whole genome shotgun sequence.
ACCESSION   DAOQUE010000021 DAOQUE010000000
VERSION     DAOQUE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6298_length_11555_cov_6.815826"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PKH10_01315"
     CDS             complement(<1..281)
                     /locus_tag="PKH10_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase domain-containing protein"
                     /protein_id="HNT26792.1"
                     /translation="MVLSLHRARAGSYYEKDAYYAKDYEQAEFQGTGAKEIGILGQPY
                     SESLYHKLYEGELPDGTRIERKFRLIEAKDDPGGPPKGELVHAGGQDLT"
     gene            complement(287..2104)
                     /locus_tag="PKH10_01320"
     CDS             complement(287..2104)
                     /locus_tag="PKH10_01320"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HNT26793.1"
                     /translation="MNNITEYLTEGGKHLFDTNHGSFYVLFFLALLTVHTAIIFGIPH
                     ENSAQLGKVTFLLAVAIPLIVYLVRIRYRKAIFKTAASRLWWIVSRTLCTAIFLVFCY
                     VSGMGRMLVILLTIIFYVMAYFLIKRRINKPKNNEEYIRGSRMTGAEQLAKELEKAGK
                     KGPLLLAGVPLPLHSETRHFFLGGSIGSGKSLGIKELLAQARNAGQRAIVYDISGEFV
                     EVFYRKDKDIIFNPLDKRSVITKGWSLFKEIDQMYDLDSVAATLIPQSKGDASPYFIE
                     AAQSVLASLMKRLMETGQETNERLYYYVAKAPIEELAAILEGTEGSSHIGSGTDPKTA
                     SNVRSTLTTKLRILGYLKDCDAASDFSLRKWVTENSDSWIFITSRQAQQATLAPLLAF
                     AIDRIGTFLLSLEPDRTRRIWISLDELSSLPRVQIETIMDKGRKYGACVLIGLQNINQ
                     LRDVYGEETAASLLSNCLTWVIYRTSDPTTAEYMSKKLGEQEIYEYVASTSQKGSLVV
                     ETNGKNITKQRTKKEIVLPSEIMNLDERIAYIKVEGNFPVVKTEFPIIQRKAQAPAFE
                     AVDFSIQRGTPAAKRTEKEYPVLAPEDGTLSDDDEPPVG"
     gene            complement(2104..2733)
                     /locus_tag="PKH10_01325"
     CDS             complement(2104..2733)
                     /locus_tag="PKH10_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26794.1"
                     /translation="MTEKKRRHNIMLSVSATDIIKARSAGPLSKSAAIEDLIEKGMRW
                     EREQKARESGEQSRPDGNSLAALERIESQIAVILERQKQAATLSEIRSNTQTEAATAI
                     IDTVKRTNNNMVALYDRMEVVIRFVGTIIPRALNLLLLYSCHVHFKSKYLHAGAFTKE
                     QLDEIKKNIDAKYNDRVCPVLDIKEYDKEDEDTTGMKEQKPDNKGAKKP"
     gene            complement(3251..3478)
                     /locus_tag="PKH10_01330"
     CDS             complement(3251..3478)
                     /locus_tag="PKH10_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26795.1"
                     /translation="MARLSKGTIETARISFEVSKPVFEALQKQRNEIKENNYPVEYDW
                     DRIVLREITSMKKVIAIHKGEAPAPKKKGGK"
     gene            complement(3874..4920)
                     /locus_tag="PKH10_01335"
     CDS             complement(3874..4920)
                     /locus_tag="PKH10_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNT26796.1"
                     /translation="MKPLLKDIYARLDRSRLLAELSPELSGDHLILTCPSCGKREAYI
                     YLNSHLIICNRQNNCGYRKSLWDWTATRYGYGQKETFAHLARLAGITPDTGAGPYPDP
                     TTLLRNTQKTCAHALLTIPDNPAFRYLHETRHWNEEEIRASGIGYHPGLDPLRLALNA
                     DHSTEEIDALPLIATKLFATKYRLIIPYHRDGVISGFIGRLLKDDPDLPKYRFPKNTK
                     VPLVDFEPTPEVTIVEGILDARLARVRGLRSVFASCTNQLSGSQLDTLTANRHIVKRI
                     RLLFDNDTAGRKGTFTSIVKLLGAGFTDIRVGELPAGHKDLEEYLSRTQATTLDAVSF
                     FPYYDWLKQYSPVP"
     gene            6497..8104
                     /locus_tag="PKH10_01340"
     CDS             6497..8104
                     /locus_tag="PKH10_01340"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HNT26797.1"
                     /translation="MMAPKKKSATKRNLPARRDEPTALSIKIPDIIVKEEVYQLEKDD
                     SLIAYEFNLIELPFFSKDDHIEANVSKKYVFSPNSYMQVFPSSDEASGQKILMDFDEK
                     VFYGIMKLYKKQGKKIITNIHELLSLSGVQVTGVNYERAKSSIYRMKGCQIRFSNSLY
                     SAEERSMLKQDKTISIIQEAELVSHEKLEDMGAKERSKYEKYFNGRIKEIVAITLSDF
                     IAANIESKGFLTYDADKLIDIGNASARKIYVMIKKWQGWEKKDILKRSCRFIASRVPL
                     SWEYSNIPSTVRNIDRSCKILKDKGLIEDYLMERAKPLKDSMITFFFSGSEDSKREEI
                     IRKNNLNNVVTTGHEELSILDVQDFLDEKQTSIFDLTTVKGEVSERMVSLLPEVARTD
                     GIRQEIERYLKEKGPVYVESNIRYSLANAKDSLAGYLVQSLRKDYGAGMRLVMETERK
                     KKTEATEEKRIEEEKKLRQEKTVKRRDYLRRFYELMSEEDKKMVQREAMELASQQGLT
                     TPKSIQLAVETTFLFIVVEKWLSEENA"
     gene            complement(8233..8604)
                     /locus_tag="PKH10_01345"
     CDS             complement(8233..8604)
                     /locus_tag="PKH10_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26798.1"
                     /translation="MGSNKKLDRLKRQAAVDEKLAAEIVGDASVNRNKQPEKQPEKKP
                     EVKAKAEPKPEIKPAPKPEKKPAPAVMLPASPKFSTNIKIDKKLWLVGKTIAIEHYDT
                     RSFSSMIENLIEEKALQLKLK"
     gene            complement(8583..9323)
                     /locus_tag="PKH10_01350"
     CDS             complement(8583..9323)
                     /locus_tag="PKH10_01350"
                     /inference="COORDINATES: protein motif:HMM:NF018691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNT26799.1"
                     /translation="MKQKTKKPIIITVGNIKGGVGKTRIATGLATMFAHDKKKVLLVD
                     SDIQGSSLTYHKRRRDLADELEKDPSVKKELNLAYDRIPIFQCFQLRSTTIDKDLRDY
                     DHDIIIVDAGGRDDEEFRASVFAADFLIIPLKPAMDDYDQTLVTIGIWRKMLRTGKKI
                     KAALVQNQLIPNKMVRFYATFNELIHKVIKDNDLKQFSPILQRLPYIESASFGLNVNE
                     TSEEGYNKAAEEFTAFYKEVSRWVATKS"
     gene            complement(10133..10660)
                     /locus_tag="PKH10_01355"
     CDS             complement(10133..10660)
                     /locus_tag="PKH10_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26800.1"
                     /translation="MAGSILFQQLMEMVMNGKNQEFDKYRHEAFPRHAFGIYREHTVL
                     AGRITHWTLEKDKEVEHLVFENEKAVDLGIRNALQTLNNKNSSIYQRHHAKTIFEELR
                     TFYKNTIVTYKTESPAIFLCCADLDRDFPFDLKPLNPVRRYAFNTAREALGLTSTKED
                     YEGWLGDIQKNERRL"
     gene            complement(10846..11271)
                     /locus_tag="PKH10_01360"
     CDS             complement(10846..11271)
                     /locus_tag="PKH10_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNT26801.1"
                     /translation="MQRNLLNISKRVNLALHALGYIGAYGRNTSAKRMAKDLPVSGTY
                     LSKVLQQLVKAGYIRSKRGAGGGHVLAKDPQNISAYEIVLLMEGPFSPDSCLFAHSYC
                     SEKDGVFHETANKVRKLMERSLRKITVARIMKNLQGRIG"
     gene            complement(11322..>11555)
                     /locus_tag="PKH10_01365"
     CDS             complement(11322..>11555)
                     /locus_tag="PKH10_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26802.1"
                     /translation="RPPPGPRRRSRRPRHHLPHNGTHYLFFGRRFGRLEGLVLVTDPW
                     SDTIITAYRNRDFPRWIKSRIKTNNPHSTDQRI"
BASE COUNT         2469 a         2726 c         3318 g         3042 t
ORIGIN      
        1 aaggtcaggt cctgtccccc ggcatggacc agttctcctt tcggcgggcc gccggggtcg
       61 tctttagctt cgatcagacg gaatttcctc tctatccgtg tgccatcggg aagttcccct
      121 tcgtataact tgtggtagag gctttcggag tatggctgtc ccagtatccc tatttctttc
      181 gcgccggtcc cctgaaattc ggcctgctcg tagtctttgg cgtagtaggc gtccttttcg
      241 tagtaactgc cggcgcgcgc ccggtgaagg gataggacca tgacccctac cctaccggcg
      301 gttcatcgtc atcggaaagg gttccgtctt ccggtgcgag gaccggatat tctttctcgg
      361 tccgtttcgc cgcgggcgtt ccgcgctgta tggagaagtc gaccgcctca aaagccgggg
      421 cctgcgcctt cctctgtata atggggaatt ccgtttttac taccgggaag ttaccctcca
      481 ctttgatata ggctatccgt tcatcgagat tcatgatctc ggagggaaga acgatctctt
      541 tttttgttct ctgcttggtt atattcttgc cgttcgtctc tacgacgagg gatccttttt
      601 gactggtcga tgcgacatat tcgtagattt cctgttcccc gagtttcttc gacatatatt
      661 ccgcggtcgt cgggtcggag gtccggtaga tcacccaggt cagacagttg gacaggagac
      721 tggcggccgt ttcttctccg tatacatcgc ggagttggtt gatgttctgc aggccgataa
      781 ggacgcaggc gccgtatttc cgccctttgt ccatgatcgt ttctatctgg acgcgcggga
      841 ggctcgatag ttcatcgaga ctgatccaga tacggcgggt gcggtcgggt tctagggata
      901 agaggaaggt gccgatgcgg tctatggcga aagcaaggag cggcgcgaga gtcgcctgtt
      961 gcgcttgccg tgaggttatg aatatccaac tgtcggagtt ttccgtcacc catttccgga
     1021 gggaaaaatc gctcgcggcg tcgcagtctt taaggtaccc gagtatgcgc agcttcgtgg
     1081 tgagggtgga gcggacattc gatgctgttt tggggtcggt ccccgatccg atatgggagg
     1141 agccttccgt tccttcgagg atcgccgcca gttcttctat cggcgctttc gctacatagt
     1201 agtagaggcg ttcattcgtt tcctgtcccg tctccatgag gcgtttcatt aaacttgcga
     1261 ggaccgattg cgccgcttct ataaaatagg gactggcgtc tcctttcgac tgcgggatga
     1321 gggtcgccgc gacgctgtcg aggtcgtaca tctgatcgat ctccttgaag agggaccacc
     1381 ctttcgtgat gacgctccgc ttgtcgagcg ggttgaatat gatgtccttg tctttgcggt
     1441 aaaatacctc tacgaattca ccggaaatat cgtaaacgat ggctcgttgt ccggcgttcc
     1501 gcgcttgcgc aagaagttcc ttgatgccga gacttttacc gctgccgata ctgcctccga
     1561 ggaaaaaatg ccttgtttca ctgtgaagcg gtaaagggac gcccgcgaga agaagcggtc
     1621 cttttttccc cgctttttca agttctttcg ccaattgttc cgcgcccgtc atgcgactgc
     1681 ctcggatgta ttcctcgttg tttttcggtt tattgatcct ccgcttaatg aggaagtagg
     1741 ccattacata aaagattatc gttaagagga taacgagcat ccttcccatg ccggagacat
     1801 agcagaaaac aagaaagatc gccgtgcata gcgttctgct tactatccac caaaggcggc
     1861 ttgcggcggt cttgaaaatc gccttacggt accggatacg gaccagataa actatcagcg
     1921 gtatcgcgac cgccaagaga aaggttacct tgccgagttg cgcggaattc tcatgaggta
     1981 tgccgaaaat tatcgctgta tgcacggtaa gcagtgcgag aaaaaacagg acatagaaac
     2041 tgccgtggtt cgtgtcaaag aggtgcttgc cgccttccgt gagatattcc gttatattat
     2101 tcattatggt tttttcgctc ctttattgtc cggtttttgt tctttcatac cggtggtatc
     2161 ttcgtcttct ttgtcgtatt cctttatatc taataccggg catacacgat cattgtattt
     2221 cgcgtctata ttctttttta tctcgtcaag ttgttccttg gtgaaggcgc cggcatgcag
     2281 gtatttcgac ttgaaatgaa catggcagga gtataacaga agcagattta atgctcgcgg
     2341 aataatggtg ccgacaaacc ttatgacgac ttccatcctg tcgtagagcg ctaccatgtt
     2401 gttgttggtc cgctttacgg tgtcgatgat cgcggtggct gcttcggtct gtgtgttcga
     2461 cctgatctcc gataaggtgg ccgcttgttt ttgccgttca aggatgacgg ctatttgtga
     2521 ctcgattcgt tcaagcgccg caagactgtt gccgtctggt ctgctttgtt ctcccgattc
     2581 acgcgctttc tgttcacgct cccaacgcat gcctttctct atgaggtctt ctatcgcggc
     2641 tgacttgctt aagggccctg cggatcgcgc ttttatgatg tcggttgccg ataccgatag
     2701 cataatgttg tgccgccttt tcttctcggt cataactacc tctttttatt ggtggtgtgt
     2761 atgttcgtgt aattttcaca catataatgc atcatggcgg ttttgtgtgg tttcgggagg
     2821 ttatgacgcg aagcgtcgca acaggtgtgg ttcaatgggg gggagagggg acggctgtgc
     2881 cgttccgtca ccacatattg aaccagaggg cggcggatga tgcgaagcat cgatacggcg
     2941 accgttccta acccaacgga gtcgagtgcg cagcacgcag acgatctcat cccgttgctt
     3001 ccgcaagttc gcggtcaatc atcttacggg aatcaacgcg ggacttcttg atcttctggc
     3061 gttgtaagaa ggcctcataa gaacagcacc agcatccggg gtgcccacag gaaatactgc
     3121 ggtgttgtct gaaaaagccg atgccggttc ctttgaaacc gaatctgctt ctggagaaat
     3181 aatgcttgcg catggtcttg atggcgtggt ggcggtcact cggctttaac atcttgggcg
     3241 ggcccctact ttatttgccg cctttcttct tcggggcggg tgcttcgcct ttgtgaatgg
     3301 cgatgacttt tttcatggat gtgatctcgc gtaatacgat gcggtcccaa tcgtattcga
     3361 ccggatagtt gttctccttt atctcgttcc gctgtttctg aagcgcttcg aataccggct
     3421 tgctcacttc aaacgagatg cgggccgtct caatcgtacc tttggataac cgagccatga
     3481 ttttctccct aaaaaagtga agtaacgggc caccactatg gagcacgcga aaacctttat
     3541 catctatttc taaaaactca agaatttttt atgcggctcg aggggggagc ggtttcgggt
     3601 tcgggtgtcg agggtttcgg cgccgctgcg cgtgcggcgc cgacccagtt tcttctttta
     3661 gcaaaagaag aaattgggga aagaagaaaa cctttcttat gcccgccgaa acaaccgccg
     3721 gcgcggccgg accgggtcat caagaggacg ctgcgcaact cccctcctcc gcgaatacgc
     3781 ttcggcgggt caaacaggtg ctcgcgtttg agtggccact gatggcctct tgatgcccct
     3841 cggttgaagg cggggggaaa tagggaaaag aggtcagggg accggtgaat actgcttgag
     3901 ccagtcgtag taagggaaaa aggaaactgc atcgagggtg gtcgcttggg tccgggaaag
     3961 atattcttcg aggtctttgt gaccggcggg taactcccct acccggatgt cggtgaagcc
     4021 ggcgccgagc agtttcacga tcgaggtgaa ggtccccttc ctgcccgcag tatcgttatc
     4081 gaataggaga cggatacgct ttacgatatg ccggttcgcg gtgagggtgt cgagttggct
     4141 gccgcttaac tggttggtgc aggaggcgaa taccgaacgg agaccgcgga cgcgggcgag
     4201 gcgggcatcg aggatccctt cgacgatggt cacttcgggc gtcggttcga aatcgacgag
     4261 cggcaccttc gtgttcttgg ggaaacggta tttggggagg tcggggtcgt ccttgaggag
     4321 acgaccgatg aaaccggaga tgacgccgtc tcggtgatac gggatgatga gccggtactt
     4381 cgtcgcgaag agtttggtgg cgatgagtgg taatgcgtcg atctcttcgg tggagtggtc
     4441 ggcgttaagc gcgaggcgga gggggtcgag accgggatga tagccgatgc cggaggcgcg
     4501 gatctcctcc tcgttccagt ggcgcgtctc gtggaggtac cggaatgccg ggttgtcggg
     4561 gatggtgagg agcgcatgcg cgcaggtttt ctgcgtgttg cgcaggagcg ttgtcggatc
     4621 ggggtacggg ccggcgccgg tgtccggcgt gataccggca aggcgggcga ggtgcgcgaa
     4681 ggtttccttc tggccgtaac cgtagcgggt tgcggtccag tcccagaggg atttacggta
     4741 gccgcagttg ttttggcggt tacagattat gaggtggctg ttgaggtaga tgtaggcttc
     4801 ccgcttgccg caggagggac aggttaggat gaggtggtca ccggagagtt cgggggagag
     4861 ttccgccaaa agccgggaac gatcgagacg ggcgtagatg tctttgagga gcggtttcat
     4921 gtttcctccg gtccgaggca gtatatccga caaaaaggag gaaacaattt ttctgggggg
     4981 gatgtttcgg gtggctggtg tgaccgcctt tcgttttcat cgaataggga aagaggcgct
     5041 tttgccgggg ttaaagtggt gttattctcc tgtgttttca gtatgttctg aactccgtga
     5101 aaatggtgtc gggcgggtgt ctatgtcgtg ttcgtcgata aagtatcggg gggatgaccg
     5161 ggttccgcgc gccggtcacg ggttgcagtg aacggcgcgg atcgggtcga accacgataa
     5221 ttccgggcga caccggtatc ggggaaggac ccgattcgtt gcggtgacat ctgatggatg
     5281 accgggttcc gggccgttgc cgggctggcc gcgtggcttt ggttcgggtg gagtcacgat
     5341 taccgatccc caccctcctg aacgatcccg gttcccgccg gccgcgggta ggagcggacg
     5401 gcgccgccgc aaatatcggg aggaaccacg ataccgcaaa gaggtcgtcg cctgccggag
     5461 tcggttgatt ttttcccttt ttgttgaaat tatttttgtg gggttaagaa gaagagtttt
     5521 tttgtagatt gatttatcga gcgtagcgag atgctgttgg tttgggtatg ggtgcggcgt
     5581 agccgctatc ccatccttgt tggagaatgg tggcttgttg aggttcgtgg ctttggttgt
     5641 gatatggagg tgccttgcga gaacggtagt gggagcaagg cggattgggt atgggagcga
     5701 cgataggagc gatcccatcc gaattggggg gttttgtttt cgcgcgagcc ttcgctacgc
     5761 tacggctcgc aggcgaacaa agaggctgtt ggtctttgta ttttccccgc tacgcgacga
     5821 acggcctccg gccgtggatc ggatgcaggg ttcccctact tcggcgctcc gccgccggct
     5881 tggagcctcc gccagaggct acggctggcg ttggattaaa ttttgcgcgc gcgggagggg
     5941 ctagttgtag ttatcttctg ttttctcaaa tacagagaag ttataatata gttatataga
     6001 tgctatgctc gaagtacagg ttatttatgc cggatgctct gggtttctat gccggatgta
     6061 caggttgtcg cgtttccgtt ctacagcgac aaatcggctc gatacacagt gtttctcggc
     6121 ccgacatata tctttttatg ctcgatgttt agggttgtta tgctcgaaat acacctttga
     6181 gcgatctctc gcgggtggga tatgccggaa gtacagcatc gcggtgacgg atatcggtaa
     6241 tctcctttca agagaggatt gaaatatgcc ggatgcacat gcttttatgc tagataccta
     6301 gtgtttcggg actataaaga cgaccattat gcgtgatgac cagtattttt tcttgacaag
     6361 cgctgaattc tgaggaatag tatgcctgac acacagggtt ccgggaggtc tcagtgagtt
     6421 tttatttctc tcaattcact gatattacaa tgattcttgt agttcctcgg ctcgatatac
     6481 agggtagtga ggggtgatga tggcaccgaa aaagaagtcc gccaccaagc gcaatctgcc
     6541 ggcacgacgc gacgagccga cggcgctgtc tattaagatt ccggacatta tcgtcaagga
     6601 agaggtctat cagcttgaaa aggatgatag cctcatcgct tatgaattca acctgatcga
     6661 gctccccttc ttctcgaaag acgaccatat cgaggccaat gtttccaaga aatatgtgtt
     6721 ttcccccaac agttatatgc aggtgtttcc gagttcggac gaggcaagcg gccagaagat
     6781 cctaatggac ttcgacgaga aggtgtttta tgggatcatg aaactctaca agaagcaggg
     6841 caagaagatc atcaccaata tccatgagct cctctccctt tcgggagtcc aggtcaccgg
     6901 ggtcaactat gagcgcgcca aatcgagtat ctaccgaatg aagggctgtc agatccgctt
     6961 ctccaattct ctctacagcg ccgaagaacg ctcaatgctc aaacaggaca agaccatcag
     7021 catcattcag gaggcagaac tcgtttctca tgagaaactc gaagatatgg gcgcaaaaga
     7081 acggtcgaag tatgagaagt actttaacgg ccgcatcaaa gagatcgtcg ccatcaccct
     7141 ctccgatttc atcgcagcca atatcgagag caagggattc ctgacctatg acgccgacaa
     7201 gcttatcgac attggcaatg cgtcggcccg aaagatatat gtcatgatca agaagtggca
     7261 ggggtgggaa aagaaggata tcctcaagcg gtcgtgtcgg ttcatcgcaa gccgtgtccc
     7321 tctctcttgg gagtattcca acattccctc gaccgttcgt aacatcgatc ggagttgtaa
     7381 aatactcaaa gacaaggggc tgatagagga ctacctgatg gagcgggcca aacctctcaa
     7441 ggacagtatg atcaccttct tcttctctgg gagcgaggat tccaagcgcg aggagatcat
     7501 ccgcaagaat aacctcaaca atgtggtgac tacgggccat gaagagcttt ctattctcga
     7561 tgtacaggat ttcctcgatg agaagcagac aagcatcttc gatctgacca cggtaaaggg
     7621 cgaggtatcg gagcggatgg tgtctctcct gcccgaagta gctcgtaccg acgggatccg
     7681 ccaagagata gagaggtatc tcaaggagaa ggggcccgtc tatgtagaat ctaatatccg
     7741 gtactcgctc gccaacgcga aggactccct cgcggggtac ctcgtgcagt cgctccgcaa
     7801 ggattacggt gcgggcatgc gcctcgtcat ggaaacggaa cgaaaaaaga agacggaagc
     7861 gacagaagag aaacggatcg aagaagaaaa gaaactgcgt caggaaaaga ccgtgaagcg
     7921 ccgcgactat ctcagaaggt tttatgaact catgagcgaa gaggataaga agatggtcca
     7981 gcgtgaagca atggaactgg cgtcgcaaca ggggttgacg actccgaagt cgatacagct
     8041 ggcggtggag actaccttcc tttttattgt ggttgagaag tggctctccg aagaaaatgc
     8101 ttgagagggt cccctcccct actccgaggg ctccatggtc ggcgttccgt tctcctagga
     8161 agcctcagtt agaagtgcat ttataaatat acctggggtg gtcaatgtgt tttgccacct
     8221 atcgacagcg ggctatttga gtttaagttg gagagctttt tcttctatga ggttctcgat
     8281 catggaggaa aacgaacggg tgtcgtagtg ttctatggcg atggtcttgc caacaagcca
     8341 gagtttcttg tcgatcttga tgttggtgct gaatttcggc gaggccggga gcatgacggc
     8401 gggggccggt ttcttttccg gcttgggtgc tggtttgatt tccggcttgg gttccgcctt
     8461 ggccttgacc tccggctttt tctccggctg cttctccggc tgcttgttcc tgttcaccga
     8521 agcgtcgcct acgatctcgg cagcgagttt ctcgtcgacg gccgcctgtc ttttaagccg
     8581 atctagcttt ttgttgctac ccatcgggaa acctccttat aaaatgcagt aaattcctcg
     8641 gctgccttgt tatacccctc ttccgatgtc tcattgacat tgaggccgaa actggccgat
     8701 tctatatagg gtaatcgctg aagtatgggc gagaactgtt tgagatcgtt gtccttgatg
     8761 accttgtgta tgagttcgtt gaaggtggcg tagaaccgaa ccatcttgtt cgggatgagc
     8821 tggttctgga caagggctgc ctttatcttc ttcccggtgc ggagcatctt gcgccaaatg
     8881 ccgatggtga cgagcgtttg atcgtaatcg tccatcgccg gcttgagcgg gatgataagg
     8941 aagtcggcgg caaataccga agcgcggaat tcttcgtcgt cacggccgcc ggcgtcaacg
     9001 atgatgatgt cgtggtcgta atcccggagg tctttgtcaa tggtggtcga gcggagctga
     9061 aagcattgga agatcgggat acggtcataa gcgaggttaa gttctttttt taccgaagga
     9121 tctttttcca gctcatcggc cagatcgcga cgccgcttat ggtaagtaag cgaagacccc
     9181 tgaatgtcgg aatcgaccag tagcaccttt tttttgtcat gggcgaacat ggtggcaagc
     9241 ccagttgcga tgcgggtctt ccctacccca cctttgatgt tcccgaccgt gataatgatc
     9301 ggtttctttg tcttttgctt catgcacacc tccacacatt acaacctaga tacatggcca
     9361 cacagcgaca gaatgacata gacacaaata attgtcaaga aaatccgaga agccccgtcg
     9421 agcgtccctt cccctgttct attttgtgta ttgaaaaacc cgtccttgga attgccaaaa
     9481 gggtgtttat attcattagt atatttacac acagccatac taccacacag acacttgtac
     9541 atattggcgc atagacatag tttgttgtgg catcgccgcg atcgatgccg cctcttaact
     9601 taacaacatt tatgctttaa catttagaca ccataacaca cagccatatt tgtgttttgc
     9661 cacacagttc tttgagacaa gcgggcgtgc atcatgcggc ttctcgcaat agcgacattt
     9721 atattattac acataacaac atatgtgtgt agccacatgg atatacggca aaacccgaga
     9781 ccatgccata ctttcgcaag ccgctcagat atctctgcta ggaccttgtg gggcatcttg
     9841 tcttcagcga tggagtgggg gagggctcgg tcgcgaggcg cgtagcgaag aacaagaagg
     9901 atatgatgac gaggaaatgc gatgtctatg ttttcttgtg cttgtgtttt tatgtggctt
     9961 tagatttgtg tgtctatgta tatttgttgg ttattgacga tcgatgctcc ggcctcttta
    10021 agagaatcgt agctttataa tcgtttgccg agggaagtct ctcgtcgtac gccgggaccc
    10081 cgttgtctga gaaaaaaagg gggggggggg ggcggacccc ttcccggaaa cttcagaggc
    10141 gtctttcgtt tttctgtata tctcccaacc agccttcata atcctccttg gtcgatgtaa
    10201 gaccaagtgc ttcgcgagca gtgttgaagg cataccttct tacgggatta agcggtttta
    10261 gatcgaaagg aaagtctcgg tctaggtccg cgcaacaaag aaagatggcg gggctttctg
    10321 ttttgtaagt cacgatggtg tttttataga aagttcgcag ttcttcaaat atcgttttgg
    10381 cgtgatgccg ttgatagata gagctgttct tgttgttaag ggtttgcagg gcgtttctga
    10441 taccaaggtc gacagccttc tcgttttcaa agacaagatg ttcgacttcc ttgtcttttt
    10501 ccagagtcca gtgggtaatg cggcctgcaa gaacggtatg ctcgcgatag ataccgaaag
    10561 catgtcgcgg aaaggcctca tgccgatact tgtcgaactc ttgatttttg ccgttcatca
    10621 ccatctccat caattgttgg aaaagtatgc tacccgccat acggcaacgc acagtatcat
    10681 acgagattgg cgaatcaatt tttttgataa taacagatgg aatttctgga gcagactggt
    10741 cgcctcagag agctggccgg ggtgcgaggc tttgtattga gtggcaggca ctgccgcccg
    10801 tgttcctctg attgttgttg atatggaagg ataaggggaa ggggcttagc ctatacgccc
    10861 ttgtaaattc ttcataatgc gggcaacggt gatcttgcgc agagaccgtt ccatcagttt
    10921 ccgtaccttg ttggccgtct cgtgaaagac accgtctttc tcagagcagt acgaatgggc
    10981 aaacagacaa ctgtcgggag aaaaaggacc ttccatgaga agcacgatct cataggcgga
    11041 aatgttctgg gggtctttcg cgagaacatg tcctcctccg gcgccccgtt tcgatctgat
    11101 atagccggcc ttgaccagtt gttgcaagac cttcgacaaa taggtcccgg aaactggaag
    11161 atcttttgcc atccgtttgg ccgatgtatt acggccgtag gccccgatat agccaagggc
    11221 atggagtgcg agattgactc gcttcgagat gttgagcaaa ttgcgttgca tggagtatcc
    11281 ctcccgtcaa acggtttatt atacccggtg aaatgcaggg atcaaattct ttggtcagtg
    11341 gagtgggggt tgttggtctt tatgcgtgac ttgatccaac ggggaaagtc gcggttccgg
    11401 taggccgtga tgatggtgtc gctccaagga tcggtgacga gcaccagccc ctcgaggcgg
    11461 ccgaagcggc gaccgaaaaa gaggtaatgg gtcccgttgt gtggtaggtg gtggcgaggt
    11521 cggcgagacc ttcgtcgagg acccggtgga gggcg
//
LOCUS       DAOQUE010000022        11396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_6400_length_11396_cov_4.039591, whole genome shotgun sequence.
ACCESSION   DAOQUE010000022 DAOQUE010000000
VERSION     DAOQUE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6400_length_11396_cov_4.039591"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKH10_01370"
     CDS             <1..424
                     /locus_tag="PKH10_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26803.1"
                     /translation="LRAPSSDASPVGAGPERMAAKMDKFRDIGNLIMATGGFLFLPCE
                     CGLRMKVPQTLGKTSVPCPRCGRILILPSVTAGVTHQGAEVKKKDDPTGEPPSVYERK
                     SEGWESFSCRCGYPIHLSPLFRSTAVTCRNCGRTIDIR"
     gene            complement(429..746)
                     /locus_tag="PKH10_01375"
     CDS             complement(429..746)
                     /locus_tag="PKH10_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26804.1"
                     /translation="MPTDDLNNLIGLDDASLLTMIIDGSLTRNRSYEFFSTERGKTIH
                     RQAKTLQGVLLDMKNGAKVVSEGKDEWGWRIVLENAETRYRRTVIMSPDMHDIFRQRK
                     KRI"
     gene            complement(769..1761)
                     /locus_tag="PKH10_01380"
     CDS             complement(769..1761)
                     /locus_tag="PKH10_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNT26805.1"
                     /translation="MKALLLSAGLGTRMRPMTDRIPKPLLPVLNVPVIEYNINFLKKH
                     GVTDLYINIHHCPEQIKEHLGDGGRWGIRIAYLFEKEILGTGGAIASMKDIATERFIV
                     MNADTIVDFDIEKMLDFHIAQKALVTLGLLRAREGQQHAVVAASGDGRLIRMLGGTPF
                     ATLPPANAIFSGVHLIEPALFDYIPHGIYSSITSEIYQRLVCERSDIVGWFIDGLWYD
                     IGTPDSFVAASFDLLSRLPLSFPVPQLVGTQGLTTDQLVLLGKKSKIPAVPVYPPVII
                     GEGVKVTGAKEVGPWLVVGPGVSIKSDGPVTNAIYLPGAKGEAVIRTERGRIHY"
     gene            complement(1758..2804)
                     /locus_tag="PKH10_01385"
     CDS             complement(1758..2804)
                     /locus_tag="PKH10_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNT26806.1"
                     /translation="MSDRALEYAVFSLLKDWKGVPMYDLSIEPLFGDASGRLYHRVRF
                     GKVSVILMTVAHTKPGEFGRGDAWADFLGMQKFLAAAGLPVPAVLHTDPEERRALLED
                     LGDLTLFAHISADPSRKMKDLRMALDLLISWQRAMWKRRSFNSPADGRSFTTTLFMEE
                     FYHFYEYMIERRVYHPSYKGLWRKLEKRFRRIAQELGNAPALVSHRDFQSKNIMIVKG
                     RPRLIDFQDALLAPIVYDPVALLRDSYVELSADELDHLLDHYWRYNEVARESLPDAAA
                     FRRLFFLQTLQRKMKDAGRFIYLHQVKGKEWFVPFVRPSLFYVRDALVRLGLDDLREL
                     LAPYIPELGDGGRS"
     gene            complement(2801..3439)
                     /locus_tag="PKH10_01390"
     CDS             complement(2801..3439)
                     /locus_tag="PKH10_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26807.1"
                     /translation="MTEETLRSDRLRVAAALDQAWIQYRANFARLIPFGMLAVLPPLG
                     FLHSLPLGLALLFGVEGTLVMMLVDAVHRADTEEQPDLLRRCAHRAWHNIKNGTVILL
                     FVLPLLLVGFAALIVPSVLFFSFFLFSFHHAAVRDKFAIDACMESYRNGRGLRGPLFL
                     VALSFYLVIGVLFLVAYQLSGGAGWLAVLPCAFFLPYYFLVIEELFQRLEQS"
     gene            3572..4957
                     /locus_tag="PKH10_01395"
     CDS             3572..4957
                     /locus_tag="PKH10_01395"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNT26808.1"
                     /translation="MKTFLTAFLISFAFLIVVTPILIHYGRRWGVVDASNRRTIHSGN
                     IPRIGGVGISLGTMLPLILLFFYHNNVSEVFFSTLANPLVIIGGGLLISGLGLLDDVR
                     GVPARWKFLFQIAVAAAAYRFGFAIDQLNTPFGVIEFGYLSLPVTILWIVGIINAFNL
                     IDGMDGLSSGIAFFVSVTILILALHNNIEFVALISAALAGAVVGFLVYNFNPAKIFMG
                     DSGSMFIGFLLAVLSLKGASKHHTIVSLLVPIVAMGVPILDTALALIRRFLRSQPLFM
                     ADRQHIHHVLLSKGWNQRRVVLTLYGITILFTALALFSVFLKDREGFLIIAVFSVIIV
                     VIITKLGYLDILLGRYRLNRQNRMEDLLERMFVERLSQMPLREMEEFIFSLPIQGFAL
                     ITADGETIFESGTKDPVNFLDIPAERDHFLRLHWKGVVPAINTRESQMFTVVAKSLFL
                     FICGEKRDEMP"
     gene            5024..5254
                     /locus_tag="PKH10_01400"
     CDS             5024..5254
                     /locus_tag="PKH10_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT26809.1"
                     /translation="MTRLVKDRQLLSRLKEYRENNLISKTELARKAEISLVTLDRIEK
                     GYACRMATKRKILQALGIDIPGADPLSPEEDR"
     gene            complement(5264..6151)
                     /locus_tag="PKH10_01405"
     CDS             complement(5264..6151)
                     /locus_tag="PKH10_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNT26810.1"
                     /translation="MLRIDKVAGRDTLMESEIPFLKVFKKGKVREVYELGAYLLIVAT
                     DRLSAFDVVMKSGIPHKGVYLTKQSSFWFKFLNQKGYLTHFVTDDIAEMAKLTGEKRL
                     CDLPWLKGRAMLGKKAELVPVEAVCRFRVFGSAVPALKEKKWIWDAPEIDGEIKEGAL
                     IKNGPIFTPTEKSETDDKISFEQMVKIVGDKARAEEIKNKTVEIFRIVNDHAQKTFNF
                     EIADGKIEWGLHGNELMLIDETFTSDSARFMPDKSKEFFRSWLKDHNLKGQAVALPDE
                     VAMAVADLYAAQCRAMRIL"
     gene            complement(6177..7202)
                     /gene="prs"
                     /locus_tag="PKH10_01410"
     CDS             complement(6177..7202)
                     /gene="prs"
                     /locus_tag="PKH10_01410"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNT26811.1"
                     /translation="MEQSRGKLGIMTCGSGKAFADRVVGHIESIYGKRLAESGFKYLL
                     SEEVRFANGELKTVLHEPVRGYDLYILQLMDDPLSPRSVNDNLMALATAVNAAYNSDP
                     NSVTAVIPQFPNSRQERRKGRESLTAAMVAQFLEECGAGRVLTIDIHAEAIVGFFRKA
                     RLDNLHASRQIIHYLHENDLISPETVVVAPDVGSAETARFYSRELKRDMAMIVKERDY
                     SKTSTVISTRLVGNVKERDILIIDDMIATGGTLIAACRTLKEFGARRIDVAATFPFFN
                     GPAAQNIDAAWHEGIINTVAGTNAVWHGEEFVKKHPWYREVDVTRLFAEVIYRINHMK
                     SISELLD"
     gene            complement(7248..8339)
                     /locus_tag="PKH10_01415"
     CDS             complement(7248..8339)
                     /locus_tag="PKH10_01415"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HNT26812.1"
                     /translation="MTSTTMPVLRIGDLTISPPIIQGAMGVRVSRSGLAAAVANEGCV
                     GTIASVGLGDYEHASDAHLNTVSNEALRDEIRKARALAPTGIIGVNVMVVLDNFPGLI
                     GVCVEEEVDLIVCGSGLPLDLPKYLNGKDIKLVPIVSSARALNIICRKWKQHYDRLPD
                     AVIVEGPEAGGHLGYSFDELLDHTAPTLEQLVTELVAFTRTLDRPIPVIAAGGIFDGA
                     DIAKFLKLGAAGVQMATRFVCTDECDVHPRFKQAYLDAGKEDTIIINSPVGLPGCVIK
                     SAFVDRVHAGGEVPFICRHHCLRTCDPNTVPYCIAKVLGDASRGDLANAFVFAGSNAY
                     RCTEIVPVKRLIAQLSAELLANLPGTDHA"
     gene            complement(8378..9415)
                     /locus_tag="PKH10_01420"
     CDS             complement(8378..9415)
                     /locus_tag="PKH10_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT26813.1"
                     /translation="MKVLITGGAGFFGLHMARKCVAEGDAVTLLDIAEYRADEYDPSV
                     TLVHGDVRDTEKMDGLVAAADVVIHAAAALPLESRTEIFSTNVDGTRNVLDLALKHKT
                     ARFVFISSTAVYGVPDHHPLHETDERIGVGPYGESKIAAEVHCEEVRAQGLYVTVIRP
                     KTFIGTHRLGVFQILYNWIEEGHKIPVIGNGKNRYQLLEVDDLCDAVFKAYRHPGAGA
                     NDVFNVGCAEFRTINEDVGALCEYAKSGARVMHTPVWLVIPALQLFEALNLSPLYKWV
                     YGTAHKDSFVSIEKARRTLGWRPIYSESEALIRSYQWYLDNKGTIAKETGTSHRVAWK
                     QGILSFFKKFL"
     gene            complement(9420..10295)
                     /locus_tag="PKH10_01425"
     CDS             complement(9420..10295)
                     /locus_tag="PKH10_01425"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNT26814.1"
                     /translation="MLTILRLLRVHQWVKNLLIFFPLFFALRFTDTGLLVRACAAFIF
                     FSLTASAVYVVNDLRDIAEDREHPDKRRRPLASGAISHVAAVFIAASLFLAGLGGASF
                     YNRQLFLILAIYCALNVLYSMLFKRIALLDIFVIATGFLLRLIAGTEYAGLAGVHPSQ
                     WIIVMTFLLALFLALAKRRDDVLLAGEGKRTRHAVHGYNLEFINSALAVMTAVIILAY
                     ILYTMDPAVARHFGSDKIYLTVIFVILGLFRYLQLTLVHKKSSQPTELLLRDRFLQLT
                     ILGWIALFVILVYLR"
     gene            complement(10363..>11396)
                     /locus_tag="PKH10_01430"
     CDS             complement(10363..>11396)
                     /locus_tag="PKH10_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26815.1"
                     /translation="DQDRESRVPGPLETVDIALAGGRDDGTLRPEAEARELGQPRGIP
                     FRSRQVIRETDEGAAFTYHGGAVGSPDDGTCGNAILIGALPFSVNGDLADRTNGLSLY
                     GAGCAAATQDTNDIVYRLSVEGGRTYQMEATPTDAGDIALNLLGACGEEAVCFDSAGA
                     AAVTYTPAQDRTVHIALEGTGTYTLTVTVEEIPDEEETDDSGTPDDAGDPDEDFPPTG
                     EIETDHDVTIDDVLPDEIVIDDMATDDTLIDEEGTDDDGTPLVDDAVIPDDAVPTDNG
                     PVADTDTATPDADTTEPDTSADKDTPVADDEQEDETVTDGILPEEDSPRPDELNKPEN
                     DGCGCSLVF"
BASE COUNT         2077 a         3389 c         3596 g         2334 t
ORIGIN      
        1 cctgcgcgcc ccctcatccg acgcctctcc ggtcggggcg ggtccggaga ggatggccgc
       61 caagatggac aagttccggg atatcggcaa tctcatcatg gcgaccggcg ggttcctgtt
      121 cctcccgtgc gaatgcggac ttcgcatgaa ggtgcctcag acgctcggca agaccagcgt
      181 cccctgtccc cggtgcgggc gtatactgat cctgccgtcg gtgaccgcgg gggtgacgca
      241 tcagggggca gaggtgaaaa agaaggatga cccgaccggc gaaccgccgt ccgtctacga
      301 aaggaaaagc gaaggatggg agagtttctc ctgccggtgc gggtatccca tacacctgtc
      361 gccgctgttc cgttcgaccg cggtgacctg ccgcaactgc gggcggacca tagatatccg
      421 gtagggtgtt agatgcgttt tttccgttgc cggaagatat cgtgcatgtc gggactcatg
      481 atgacggtgc gacggtaacg ggtctcggcg ttctcgagta cgatgcgcca cccccattcg
      541 tccttgccct cggaaacgac cttggcaccg ttcttcatgt cgaggaggac cccctgcaag
      601 gtctttgcct gacggtgtat cgtcttgccg cgctcggtgg agaagaactc gtaactacgg
      661 ttcctcgtga gcgagccgtc aatgatcatc gtgaggaggc tggcgtcatc gagcccgatc
      721 aggttgttca gatcgtcggt cggcatggcg tgttcctccg ctcacccgtt aataatggat
      781 cctgccgcgc tcggtccgga tgaccgcctc gcctttcgcc ccggggagat agatggcgtt
      841 cgtgaccggg ccgtcgctct ttatcgaaac gccggggcct accaccagcc acggccccac
      901 ctccttggcc ccggtcacct tgaccccttc cccgatgatg accggcggat agaccggcac
      961 cgccggtatc ttgctcttct tgccgagcag gacgagttgg tccgtcgtaa gcccctgcgt
     1021 tcctaccagt tggggtaccg ggaaggaaag aggcaggcgg gagaggaggt cgaacgaggc
     1081 ggcgacgaac gagtcgggtg tcccgatatc gtaccagagg ccgtcaatga accagccgac
     1141 gatatcggac cgttcgcaga cgagccgttg gtatatctcg gaggtgatgg acgagtagat
     1201 gccatggggg atatagtcga acagggccgg ttcgatgagg tgaacgccgc tgaagatggc
     1261 gttggcgggc ggaagcgtcg cgaagggggt gccccccagc atccgtatga gccggccgtc
     1321 gcccgacgcg gcgaccacgg cgtgttgctg cccttcacgg gcccggagga ggccgagtgt
     1381 cacgagcgct ttctgggcga tatggaagtc gagcatcttc tcgatgtcga aatcgacgat
     1441 ggtatcggcg ttcatgacga tgaaccgctc ggtggcgatg tccttcatgc tcgcgatggc
     1501 gccgccggtc cccaggatct ccttttcgaa caggtaggcg atgcggatgc cccagcgacc
     1561 gccgtcgccg aggtgttctt tgatctgttc ggggcagtgg tggatattga tgtacagatc
     1621 ggtgacgccg tgttttttga ggaagttgat gttgtattca atgaccggga cattaagcac
     1681 cggcaggagc ggtttgggta tccggtcggt catcggccgc atccgggtcc cgagtccggc
     1741 ggagagcagg agcgccttca tgagcggcct ccgtcgccga gttcggggat atagggagcg
     1801 agcagttcgc gcaggtcgtc caagccgagc cgtacgagcg cgtcgcggac atagaagagc
     1861 gaggggcgga cgaaggggac gaaccactcc ttccccttga cctgatgcag gtagatgaac
     1921 cgtccggcgt ccttcatctt gcgctggagc gtctgcagga agaacaggcg ccggaacgcc
     1981 gcggcgtcgg ggagcgattc gcgcgccacc tcgttgtatc tccagtagtg gtcgagcagg
     2041 tggtcgagtt cgtccgccga gagttcgaca taggaatcgc gcaggagtgc caccggatcg
     2101 tacacgatgg gagccagcag agcgtcctgg aagtcgataa ggcgcggccg tccctttacg
     2161 atcatgatgt tctttgactg gaagtcgcgg tgactgacga gcgccggggc gttgccgagc
     2221 tcctgcgcta tcctgcggaa gcgcttttcc agtttgcgcc acaatccttt gtaggagggg
     2281 tggtagaccc gccgctctat catgtactcg taaaaatggt agaactcctc catgaaaagg
     2341 gtcgtcgtga aggagcgtcc atcggcgggg ctgttgaaac tgcggcgttt ccacatggcc
     2401 cgctgccacg agatgagcag gtcgagcgcc atccgcaggt ctttcatctt ccgggacggg
     2461 tcggccgaga tatgggcgaa cagcgtcagg tccccgagat cctcgagcag ggcgcgtcgt
     2521 tcttcggggt cggtatggag caccgccggt accgggagcc cggccgccgc caggaacttc
     2581 tgcataccga ggaagtcggc ccacgcgtcg ccgcgaccga attcgcccgg cttggtgtgg
     2641 gccaccgtca tgagaatgac gctcaccttc ccgaaacgga cgcggtgata caggcgaccc
     2701 gatgcgtcac cgaacagtgg ttcgatggaa agatcgtaca tcgggacccc tttccagtcc
     2761 ttcaggaggg aaaagacggc gtactcaagg gcccgatcac tcatgactgc tccagtctct
     2821 gaaagagttc ttcgatgacg aggaaatagt aggggagaaa aaaggcgcac gggagaacgg
     2881 ccagccagcc ggcgccgccg gacaactggt aggcgacgag gaacagtacg cctataacga
     2941 ggtaaaatga aagagcgacg aggaagagcg gcccgcggag cccccgcccg ttccggtaac
     3001 tttccatgca ggcgtctatc gcgaacttat cgcggaccgc cgcgtggtgg aacgagaaga
     3061 ggaagaaact gaaaaaaagg acggatggga cgataagcgc ggcgaacccc accagaagga
     3121 gcggcaggac gaagagcagg atgaccgtgc cgttcttgat gttgtgccat gcgcggtgcg
     3181 cgcatcgccg cagaaggtcg ggctgttcct cggtgtcggc gcggtggacg gcatcgacga
     3241 gcatcatgac gagcgtccct tccacgccga aaagcagtgc caatcccagc ggcagcgagt
     3301 gcaggaaacc gagcggtggc aacacggcga gcatgccgaa agggatcagc cgcgcgaagt
     3361 tcgcgcggta ctgtatccac gcctgatcga gcgcagcggc cactctgagg cggtcgcttc
     3421 gcaaggtctc ttccgtcatg tggtcctccg tatcgggaac ggaacatttt acccgatcct
     3481 cactgaacgg tcaactttat tattgtggtc cgcgctattt gatttttcgc ttttttccgt
     3541 tatgataccg ccggtcttgt aaggtggccg catgaagaca ttccttaccg cctttctcat
     3601 atcgttcgcc ttcctgatcg tcgtcacccc catcctcatc cattacggcc gccgctgggg
     3661 ggtggtcgac gcgtcgaacc ggcgcaccat acattcgggc aacataccgc gcatcggcgg
     3721 ggtcggcatc tcgctcggca ccatgctacc gctcattcta ctgttcttct accacaacaa
     3781 cgtatccgag gtgttcttca gcacgcttgc caatccgctc gtgatcatag gcggtggcct
     3841 gctcatcagc ggactcggtc tgctcgacga tgtgcgcggc gtacctgcgc ggtggaagtt
     3901 cctcttccag atagcggtcg cggccgccgc gtaccggttc gggttcgcca tcgaccagtt
     3961 gaacaccccg ttcggcgtga tcgaattcgg ctatctttcc ctaccggtga ccattctgtg
     4021 gatcgtcggg atcatcaacg cgttcaacct gatcgacggc atggacgggc tgtcgtcggg
     4081 catcgcgttc ttcgtatcgg tgaccatact catcctcgcg ctccacaaca atatcgagtt
     4141 cgtcgccctc atatcggcgg cgctcgccgg ggcggtggtc gggttcctcg tctacaactt
     4201 caaccccgcc aagatattca tgggtgattc cgggtcgatg ttcatcgggt tcctccttgc
     4261 ggtgctttcg ctcaaagggg cgtcgaagca ccacacgatc gtttccctgc tcgtgcccat
     4321 cgtcgcgatg ggggtgccga tcctcgatac ggcgctggcg ctcatccggc gattcctgcg
     4381 gagccagccg cttttcatgg ccgaccgcca gcacatccac catgtcctgc tttccaaggg
     4441 gtggaaccag cggcgcgtcg tgctgacgct ttacggcatc acgatcctgt tcacggcgct
     4501 tgcgcttttc tcggtattcc tgaaggaccg cgagggattc ctcatcatag cggtcttctc
     4561 ggtcataatc gtcgtcatca tcacgaaact cggctatctc gatatcctgc tcgggcggta
     4621 tcgtctgaat cgccagaacc gcatggagga cctgctggag cggatgttcg tggagcgcct
     4681 ttcccagatg ccgctgcgcg aaatggagga attcatcttc tccctgccga tacaggggtt
     4741 cgcgcttatc acggccgacg gtgagaccat cttcgaatcg gggaccaagg acccggtcaa
     4801 ctttctcgat atcccggcgg aacgcgacca tttcctgcgc ctgcactgga agggtgtggt
     4861 gcccgccatc aatacgcgcg agtcgcagat gttcaccgtg gtcgccaaat cgctcttcct
     4921 gttcatatgt ggcgaaaaaa gagacgaaat gccttgactt cggcccggtg accgcataga
     4981 ctgacgggcg tgtttattgt tttttgcgag atagagagag gtcatgacga gactggtcaa
     5041 ggatcggcaa ctgctcagcc ggctcaagga gtatcgcgag aacaacctca tcagtaagac
     5101 ggaactcgca cggaaggcgg agatatcgct cgtcacgctc gaccgcatcg aaaaaggcta
     5161 tgcctgccgt atggcgacca aacgcaagat actgcaggcg ctcggcatag acatccccgg
     5221 cgccgacccg ctttcccccg aagaggatcg ctgacccttc cttttagagt atgcgcatcg
     5281 cgcgacactg cgcggcgtac aggtcggcga ccgccatcgc cacctcgtcg gggagcgcca
     5341 ccgcctgccc cttgaggtta tggtccttca gccacgagcg gaagaattcc tttgacttgt
     5401 cgggcatgaa gcgcgccgaa tcgctcgtga aggtctcgtc gatgagcatc agttcattgc
     5461 cgtggagacc ccattctatc ttgccgtcgg ctatctcgaa gttgaatgtc ttctgcgcgt
     5521 ggtcgttgac tatgcggaag atctcgaccg tcttgttctt tatctcttcg gcgcgcgctt
     5581 tatcgccgac gatcttcacc atctgttcga acgatatctt gtcgtcggtc tccgatttct
     5641 cggtcggggt gaagatcggg ccgttcttga tgagcgcccc ctccttgatc tcgccgtcga
     5701 tctcgggggc gtcccagatc catttcttct ccttgagcgc cgggaccgcc gatccgaaca
     5761 cgcggaaccg gcagaccgcc tcgaccggga cgagttcggc ctttttcccg agcatcgcgc
     5821 gtcccttgag ccacggcaga tcgcagagcc gtttctcgcc cgtcagtttc gccatctcgg
     5881 cgatatcgtc ggtcacgaaa tgggtcagat accctttctg gttgaggaac ttgaaccaga
     5941 acgaactctg cttggtgaga tagaccccct tgtgcgggat gccgctcttc atcaccacat
     6001 cgaaggccga cagacggtcg gtggcgacga tgagcagata cgcgcccagt tcgtagacct
     6061 cgcgcacctt cccttttttg aacaccttca ggaacgggat ctcgctttcc atcagggtgt
     6121 cgcgtccggc gaccttgtca atacgcaaca tcgtgttctc tccgctgaac gaggggttaa
     6181 tcgagcagtt ccgatatgga cttcatgtgg ttgatacggt agatgacctc ggcgaacagc
     6241 cgcgtcacat cgacctcgcg gtaccacgga tgcttcttga cgaactcctc gccgtgccac
     6301 accgcgttgg tgccggcgac cgtgttgatg atgccttcgt gccacgccgc gtcgatattc
     6361 tgcgccgccg ggccgttgaa gaaggggaag gtggcggcca catcgatgcg tcgcgcgccg
     6421 aactccttga gcgtgcggca ggcggcgatg agcgtgccgc cggtcgcgat catgtcgtcg
     6481 atgatgagaa tgtcgcgctc cttcacattg ccgaccaacc gtgtcgatat gacggtcgag
     6541 gtcttcgaat agtcgcgttc cttgacgatc atcgccatgt cgcgcttgag ttctcggctg
     6601 tagaaccgcg cggtctcggc cgatccgaca tcgggggcga ccaccaccgt ttcgggcgat
     6661 atgagatcgt tctcatgcag gtagtggatg atctggcgcg aggcatgcag gttgtcgagc
     6721 cgtgccttgc ggaagaaccc gacgatggcc tcggcgtgga tgtctatcgt gagcacgcgg
     6781 cccgcgccgc attcctcgag gaactgcgcc accatcgcgg cggtgaggct ctcgcgtccc
     6841 ttgcgtcgct cctgccgcga gttggggaac tgcgggatga ccgcggtgac cgagttcggg
     6901 tcgctgttgt aggcggcgtt gaccgcggtc gcgagcgcca tgagattgtc gttgaccgat
     6961 cgcggggaga gcgggtcgtc catcagttgg agaatgtaaa gatcgtaccc gcgcaccggc
     7021 tcatgcagga ccgtcttgag ttcgccgttg gcgaaacgga cctcttccga aagcaggtat
     7081 ttgaacccgc tctcggcgag ccgcttgccg tatatcgact cgatatgtcc caccacccgg
     7141 tcggcgaacg ccttgcccga gccgcaggtc atgatcccca gtttgccgcg cgattgttcc
     7201 atggccgtac tcctcctgcc tagatatgtg aacggtctgt tatcatctca cgcatggtcg
     7261 gtgcccggca ggttggcgag cagttccgcc gacagttgcg cgatgagtct tttcaccgga
     7321 acgatctcgg tgcaccggta ggcgttcgag ccggcgaaga cgaacgcatt ggcaagatcg
     7381 ccgcgcgagg cgtcgcccag caccttcgcg atgcagtagg ggaccgtgtt cgggtcgcag
     7441 gtccgcaggc agtggtgccg acagatgaag gggacctcgc ctccggcatg gacccggtcg
     7501 acgaatgcgc tcttgatgac gcagccgggg agccccaccg gactgttgat gatgatggta
     7561 tcctccttac cggcgtcaag ataggcctgc ttgaatcggg ggtgtacatc gcactcatcg
     7621 gtgcagacga aacgggtcgc catctgaacg ccggcggccc cgagtttcag gaacttcgcg
     7681 atgtcggcgc cgtcgaagat gccgcccgcc gcgatgaccg gtatcgggcg atcgagcgtc
     7741 cgggtgaagg cgaccagttc ggtcaccagt tgttccagcg tcggcgcggt gtgatcgagg
     7801 agttcgtcga aagaatagcc cagatggccg cccgcctccg gtccctcgac gatgacggcg
     7861 tcgggaagcc ggtcgtaatg ctgtttccat ttgcggcaga ttatgttgag cgcgcgcgcc
     7921 gaagagacga tcggcaccag tttgatgtcc ttcccgttca gatacttcgg cagatcgagg
     7981 ggcagtccgg atccgcagac gatcagatcc acctcttctt cgacgcagac gccgatcagc
     8041 ccggggaaat tgtcaaggac caccatgaca ttgacgccga tgatgccggt cggcgccagg
     8101 gcccgcgcct tccggatctc gtcccggagc gcctcgttgc tgaccgtgtt gaggtgcgcg
     8161 tcggacgcgt gttcgtagtc gccgagcccc acgctcgcga tcgtgccgac acagccttcg
     8221 ttggcgacgg ccgccgcgag gccgcttcgc gaaacgcgga cccccatcgc tccctgtatg
     8281 atcggggggg atatcgtgag gtcgccgatg cgcaggacgg gcatggtagt agatgtcatg
     8341 agtgtgtcca ccgtatgttt acatgcatat catatcctca taggaacttc ttgaaaaacg
     8401 acagtatgcc ctgtttccac gcgacccggt gagaggtccc ggtctccttc gcgatcgtcc
     8461 ccttgttgtc gagataccat tggtaggaac ggatgagcgc ctccgattcg ctgtagatcg
     8521 gtcgccagcc gagcgtcctc cgcgccttct ctatggaaac gaaactgtcc ttgtgcgccg
     8581 ttccgtagac ccacttgtag agcggcgaga ggttcagcgc ctcgaacagt tggagcgccg
     8641 ggatcaccag ccacaccggg gtgtgcatga cccgcgcacc gcttttggca tactcgcaga
     8701 gcgcgcccac atcctcgttg atggtgcgga actcggcgca cccgacattg aacacgtcgt
     8761 tcgcaccggc gccgggatgg cgatacgcct tgaacaccgc atcgcacagg tcgtcgacct
     8821 cgagcagttg atagcgattc ttcccgttgc cgatgaccgg tatcttgtgg ccttcctcga
     8881 tccagttgta gagtatctgg aaaacgccga gccggtgggt gccgatgaag gtcttggggc
     8941 ggatgaccgt gacatacagc ccctgtgcgc ggacctcctc gcagtgcacc tcggccgcta
     9001 tcttcgactc gccgtagggt ccgacgccga tccgctcgtc ggtctcgtgg agcggatggt
     9061 ggtcggggac gccgtagacg gcggtcgaac tgatgaatac gaaccgcgcg gtcttgtgtt
     9121 tcagcgcgag atcgaggaca ttccgcgttc cgtcgacatt ggtcgagaat atctctgtgc
     9181 gcgattcgag cgggagcgcc gccgccgcgt gtatgacgac atcggccgcc gcgacgagcc
     9241 catccatctt ttcggtgtcg cgcacatcgc cgtgcacgag cgtcaccgac gggtcatatt
     9301 cgtcggcgcg gtattcggcg atgtcgagca gggtcaccgc gtcaccttcc gcgacgcatt
     9361 tccgcgccat gtggagtccg aagaacccgg cgccgccggt gatcagcacc ttcatcgcat
     9421 cacctcaggt agacgagtat cacgaacagc gctatccagc cgagtatcgt gagttgcagg
     9481 aaccggtcgc gcaggaggag ctcggtcggc tgactgcttt tcttgtggac gagcgtcaac
     9541 tgcagatacc ggaacagtcc cagtatgacg aatatcaccg tcaggtatat cttgtcggag
     9601 ccgaaatggc gggcgaccgc cggatccatc gtatagagga tgtaggcgag gatgatgacg
     9661 gcggtcatca ccgcgagcgc gctgttgatg aattcgaggt tgtagccgtg gacggcgtgc
     9721 cgcgtccttt tcccttcccc ggcgagcagt acatcgtcgc gtcgtttggc gagggcgagg
     9781 aacagcgcga gcaggaaggt catcacgatg atccactgtg acgggtggac cccggcgagc
     9841 cccgcgtatt cggtccccgc gatgagccgc agcagaaagc cggtcgcgat gacgaagatg
     9901 tcgaggagcg ctatccgttt gaacagcatc gagtagagga cattgagtgc gcagtagatg
     9961 gcgagtatga ggaagagttg tctgttgtag aacgatgcgc caccgagccc ggcgaggaac
    10021 agggaggcgg cgatgaagac ggcggcgacg tgcgatatcg caccgctcgc gagggggcgg
    10081 cgccgtttgt cggggtgttc gcggtcctcc gcgatgtcgc gcaggtcgtt gacgacatag
    10141 acggcgctcg cggtgagcga gaagaaaatg aaggcggcgc aagcccttac gagaagtccg
    10201 gtatcggtga acctgagggc gaagaagagc gggaaaaaga tgagcaggtt cttgacccac
    10261 tggtggacac gcaggagccg caatatagtc aacatgaccc ttctccggtg actcgtgggc
    10321 ggagaataac ggaaagagga cgaggagtca acaaaagaga cgttagaata ccagcgagca
    10381 cccgcagccg tcgttttcag gcttgttcag ttcgtcgggt cgggggctat cttcttcggg
    10441 caggatcccg tctgtcaccg tctcatcttc ttgttcatcg tcggccaccg gggtatcctt
    10501 gtcggcgctg gtgtccggtt ctgtcgtgtc ggcgtcggga gttgccgtgt cagtgtcggc
    10561 aacgggacca ttgtcggtcg gcacggcgtc atcggggatc acggcgtcat ccaccagcgg
    10621 ggtcccgtcg tcatcggtcc cttcctcgtc tatcagggtg tcgtcggtcg ccatgtcgtc
    10681 gatgacgatc tcatcgggca acacatcgtc aatggtgaca tcatgatcgg tttctatctc
    10741 tcccgtcggg gggaagtcct cgtccgggtc accggcgtcg tccggtgttc cgctgtcgtc
    10801 ggtctcctcc tcgtcgggga tctcctcgac cgtcaccgtg agcgtatagg tcccggtgcc
    10861 ctcaagtgcg atatggaccg tccggtcctg tgcgggagtg taggtgaccg ccgccgcgcc
    10921 ggcgctgtcg aaacagacgg cctcttcgcc acaggcgccg agcaggttga gcgcgatgtc
    10981 gcccgcgtcg gtcggcgtcg cctccatctg ataggtgcgg cctccctcga cagagaggcg
    11041 gtacacgatg tcgttggtgt cctgcgtcgc ggcggcgcac ccggcgccat agagcgaaag
    11101 accgttcgtc cggtccgcga ggtcgccatt caccgaaaag ggaagcgccc ctatcaggat
    11161 cgcgttgccg caggtcccgt catcgggtga tccgaccgcg ccgccgtgat aggtgaaggc
    11221 ggcgccttcg tcggtctcgc ggatgacttg acggcttcgg aatggaatcc cgcggggctg
    11281 gcccagctcg cgggcttccg cttccgggcg gagggttccg tcgtctcttc cgcctgcgag
    11341 ggcgatatcc accgtttcaa gcggccctgg gacccgggat tcacggtctt gatccc
//
LOCUS       DAOQUE010000023        11329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_6456_length_11329_cov_5.852226, whole genome shotgun sequence.
ACCESSION   DAOQUE010000023 DAOQUE010000000
VERSION     DAOQUE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11329
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6456_length_11329_cov_5.852226"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PKH10_01435"
     CDS             complement(<1..366)
                     /locus_tag="PKH10_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26816.1"
                     /translation="MSGTRFLTMLFALLLISCTPPEEPSDTDETTDSDTPVTDIDPND
                     QINFPDPVLRTCIEELTGKSSGEPIYAKDVADIEEVYCDDVSDLMGMEFFSRIKTITF
                     YNNNINSLKSLSGLYSLKYM"
     gene            complement(493..11316)
                     /locus_tag="PKH10_01440"
     CDS             complement(493..11316)
                     /locus_tag="PKH10_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26817.1"
                     /translation="MEESKLRAEFALVTKVENGTPTFGYKESILNWRENETELSEAGC
                     GTGERALKLSLWAPAIIADKGETTTDFQSANTPYTKIFCADERTGEIPVVRANREEGL
                     LYSVRVVNTTPSGLKSLHLSRNYHIFNLGGGSLTHRSIHDLSAIAYHPNLTHLNLAGS
                     PQHLVNGGEQGRELSPLIHTRKMKWLDLSDNMLQLEPPVNENDVNCRNYENWTDEPCQ
                     RRYGELRELRHMHDLQAFIARDTGIKYFNQRLDTLDRQCACGEHKDFKMPKPLKAENS
                     SAVLKKGLTENIEIQRISRCEGSSDKEIVVVGTIFHAFTAGYCSCGACNFDKMVEEAQ
                     KQVEDACQHRIITNPSNIWDVVYTGDQVDDSHPELDITDQYCELLQTEKCGSVPEKVY
                     YKLVINQGDEKPSLEQLEENVVCRVNREGEFPTATYRYGSDDSSYQVLPRCDAFCEDQ
                     YSSSPAGTFQVKTVIDGKTANKYRYQASFPRVGVSGEKLIAAYTIEVQYDKKMLERFI
                     NDYNDPNGRSPIFDDGILVSDISPRNLKLDLKYFLSGKITPIVDSINLTGGTATDDGR
                     GIVTASGAFEVAQSGPYDSIVFEIDSIKNSKNIFEGPISSWIPAYIKSVSIKDLVFRI
                     GNGTLCEKNEIPISGLLTDTCKCDIYSGECNGVKKFESDLAEQWPALEYLDLSDNPLD
                     NRSIEVAQTLSQYPDLPNGTHGDVVHHCSNRPWYKFIGRYDGIDKFKRLKGLYLENVS
                     DNRNKEDRQTAANTYGCDISNDLLYTVLPRLPRTLAYLDISGVYPLEVEYAPLFPDLK
                     NLRLRELDASHNVISQASFSDDVLDEQYDIEYLDLSGTTYINSFTFLRDMQNLRYLDL
                     SGTAMTEAVADDWDIDHFGRLDYLDISATQVRTLQWLAATPEVQSLFINGTGLYDIES
                     LDYLTKVMELGIAQHCIVKEESGLLVIDEDRIAEHTGQVAFLTGDDSMNAVCNGVQTA
                     TAVCGNGIREGSEQCDTEYIACAALNGTQYNIGYAYCLPPEASLFCTWDTSECGWTDD
                     DIVSDEDIVPQPDIDSDLICTNGIVAHSLDGADFCLCNAGYYSLGDRCIKESIHSTGE
                     TSAEAAQENSGERENNSTYIEERMDIDTASAPDVDQSSSPDEDIVPLSQMQTEGCSYP
                     TVESCFSEQYQRSRCGILFRGVCRDMMQFCLYVGGRCCIPQKDGNGKLLKICDPKYEG
                     ICEFKGSGTGFCTRKGLTGTEPYLGCRFSSASNSDCKAPVSSCRIEQTKYTCKTDEDC
                     GCFDENCTYTCRNKLETGTTPDLSTLERVCTAIACDSGALKPDPDQCAVVSSKDQSKA
                     ICVPKDLAEEMELLGDDEGGCYYLEEDDLSINEETGTLQIERICRLPDPVLKFNRDCA
                     TFIGCLQNEQATINNYVRFNVPKLPDGADPGQDMKYTDEIDGGNDTQLAYLGSFPTGG
                     RGSEDDFESETGSTEDYSITNCWLYNIDCGSGGEQQFSYYSLPLQSDESTFTVTRRSG
                     RSAASYQSAIAKVINDTVTDNILSYAITESPVGYCNEKLTDCRDYATKKYIDIVNAMR
                     LAAIYERQPIRGIREIFARLPLYAPLAMHDLNSLVNTQYRAGDQMELLLNAPIKRGIR
                     RSRNLFQEVVFYMSKTAETTEENGAHLKNGLFLQYDPNVPMAGGVDVSGFATQITPFF
                     SGETLCQVPDPNENYLSNALCGEKSASHSLSNYRMLFGRPEIDDETLSVYEYKVKTIS
                     LLKEQLLDLLLARANIEELWQERKEEALRMNPADESLYGVHDIARKTKPLLAQLSVYR
                     DYFDIQAIADIADPAILREYTKEKLPQEIAIKKARIQRFNELQAKLETDIRMSRYEDS
                     PEAQKLWRDHYPRTRYYLDEMNAEKRASLTQKERAELYPNGETEYLIDTVLRQIDHKI
                     EKILQVAAVGPQMLALPGYNTAVGDFYCFDQNINPAILYLCDSEPSFFLKQAVNLLPP
                     ETLEKTYQECLSATNNDANAFGSLSEVTGKYEGGYLRNTANLWVFSPSQGWNSILDDL
                     NNDNDLENVVLRLGNALREYQEWERSYKNKDAVAKVQEIEDYFSFGNTTFAFEEVEGE
                     YLRQEIVRKEDPLLVGKNAFTSCFGSYEDHNKRSLDVLASLGSISTHPACRNISGSVA
                     QPTTWQCFDMWEETYSAYLEEIQAVRDKMAIIEAEYKLKQAEMAARKLKAGGLLNPAA
                     PQETAEGEEEKPRNPFAIPSNLEKWFSDYQQFGVDDLNANFTYIAGAGIKNLILDPNK
                     YNPMPYMTDFHAFLPHLGKALLKHVNKSALDIALQDNPQLVELASLQLGQWMYNNLKN
                     MKKLEQMYTNAMKQVQKFDLVKLKGLVKGKGLGNLIDDEKILEIVADELMKEIITRAY
                     ADGSLTAGTNITNPADILDILKTGANFSIEAEMNNMPEISALPNVDESYLQAKAQEAL
                     LKLEQQRQQIEAVVRGYETTMQGNVNIIRNEIKSKYGAGKNFYDFIRRLASSGNPAKV
                     LTEDLIGASVLEMFLGQIFSGSLETDITFKIDGESLPCLKEYPDECREDVIDAIKDIP
                     PNELLGKVKITQNGATKTIKEYFSSPSEAVNMVLDYKRLLRQAALSSTLDYTMQTLKV
                     PNESAVMLRQIITTLMQTKSALACAPKFTGGSQPQLKCGSTVMDAGPVMSLPAGAIQL
                     NLMDAVKGIFESASVRGTAVDIQASPETSVMALTAGYIDAAFLDSIPILKNRADPDVM
                     KLRFVLSDFIAKIAYISWRSERLITRILDAVNGEYTPLKVYGEEGSSISPIETPALRT
                     EFDVFGTTNTFAETLKTFAREQGKKLLGKLIEKAEECATSSEKGCDINDFLGTLDMFG
                     KYGNSSQLGTEIKLPDLGEIMNQLMVLMDSYQDDQGMKDFFKESMADVLDIYRLSSGM
                     VTPQQLRSILLSGWSLDTGGVSLEQRPTEDCLPPSDCTGKVTSVTDSLAVSSLSVPAQ
                     AELREELTDLPFTDTLNILLTALGRAGISSLANSQAPDLSKAWGNIGNFDQVILPPVY
                     KTEFDTNLLDPNQLEFNLSVISEDAGFRAVVSFPSFNFGHLKGSVEGSLQQLLAPALA
                     QLDAYQADAANLIEITDPEYWKGLLDKMVVPGFDDDHLSALNDLKVKIDALKTATDGS
                     AITEIKYGDDGIAQIFNQKFQIATGTEGPLNEVVLPMETVQKVSYAIPQILIDPVFYQ
                     RVIKRIANETAIIAAFKEVFNGAASDPKSWLMATIGGGDAEGMPMPALISEGLSSLNV
                     ATLKQLFPENPDSSFFISVLADAVIMRAEEVFMKELFKLPFAEVAMHTSAGLHLFGFD
                     ADLFEATFYVTTRLDRDKYGRIIDLESVHPKARDKFIRRYDKKVEKFKPYTKYYFFAA
                     MTSFDDLAETAVEAGKKALKEAADAAIKTAVGMAQAEIDKQLEAISTDVKDADKFESK
                     IAKDPKLEDFKFAELEESDTINFQIGPVPAYLTYGFTFDAGIRYGIDYNYGWFRGLTV
                     GASAGPYIVAGAFLEVGAGFDYTFLSFQIGIGGHIDIFDFYAPLKVQGMLKPELDLSG
                     VGVKFYLSTSLIPELKLLAGYIYVRAKGSIGIEPIALKFNVTRTIFEWDPLIDMKFGN
                     LIPLKPLKIDLFSIGEGVKLLGKEM"
BASE COUNT         2537 a         2899 c         2852 g         3041 t
ORIGIN      
        1 catgtatttc aaactataga gccccgacaa tgactttagt gaattaatat tgttattata
       61 aaaagtaatg gtttttattc tcgagaaaaa ttccatcccc attaaatcgc tgacatcatc
      121 acaatacact tcttcgatat ccgccacatc cttggcatag atcggttctc ccgagctttt
      181 gccggtcagt tcttcgatgc aagtacgcag gactgggtcg ggaaagttga tctgatcatt
      241 gggatcgatg tcggtgacgg gggtgtcgct gtcggtggtt tcgtcagtgt cggagggttc
      301 ttcggggggg gtgcaggaga tgagaagcag tgcaaataac atggtcaaga atcgcgttcc
      361 gctcatcggc atctcctatc ggacatcaac tgttacacaa cctcaccccc gccctctccg
      421 gtgcggagag ggagagcaga gagggattct agcggccccc ttcccgttgt caaggaagag
      481 ggccgccgtt atctacattt ctttaccgag tagttttacc ccttctccga tgctgaacag
      541 gtcgatcttg agcggcttga gcggaatgag attcccgaac ttcatatcta tgagaggatc
      601 ccattcaaag atggtccgcg tcacattgaa cttgagggcg atcggctcga tgccgatact
      661 ccctttcgct ctcacataga tataacccgc gaggagtttc agttcgggga tcagactggt
      721 gctcaagtag aatttgacgc caactccgct gaggtcgagt tccggtttca gcattccctg
      781 caccttgagc ggagcgtaga agtcgaagat gtcgatgtgg ccaccgatac cgatctggaa
      841 actaaggaaa gtgtagtcga agcccgcccc gacttcgagg aacgcgcccg ccacgatgta
      901 agggccggca ctcgcaccga cggtgaggcc acggaaccat ccgtagttgt aatcgatgcc
      961 gtaccggata ccggcatcaa aggtaaagcc gtaggtgaga taggcgggga caggaccgat
     1021 ctggaagttg atagtatcgc tttcttccag ttcggcgaat ttgaagtctt cgagtttggg
     1081 gtctttcgcg atcttgcttt cgaatttgtc cgcgtccttg acatcggtcg agatcgcttc
     1141 gagttgtttg tctatctccg cttgcgccat accaaccgcc gttttgatcg ctgcgtcggc
     1201 cgcctctttg agcgctttct tgcccgcttc gaccgccgtt tcggcgagat cgtcgaaact
     1261 ggtcatggcg gcgaagaagt agtacttggt gtagggtttg aatttttcga ccttcttgtc
     1321 gtaccggcgg ataaatttgt cgcgcgcttt cggatggacc gattcaaggt cgatgatgcg
     1381 accgtatttg tcacggtcga ggcgggtggt gacatagaag gtcgcctcga aaaggtcggc
     1441 gtcgaagcca aacaggtgca gaccggcgct cgtgtgcatg gccacttcgg cgaaggggag
     1501 tttgaagagt tctttcatga aaacctcttc ggcccgcatg atcactgcgt cggcaaggac
     1561 agatatgaag aaacttgagt cgggattttc ggggaagagt tgttttaaag tagctacatt
     1621 cagcgaggac aacccttccg aaataagtgc aggcattggc atgccttcgg cgtcacctcc
     1681 tccaatcgtc gccatcaacc aactcttcgg atcgcttgcg gcaccgttga acacctcctt
     1741 gaatgcggcg atgatggccg tctcattcgc tatccgtttg atgacgcgct gataaaaaac
     1801 gggatctatc aatatctgcg ggatggcata ggaaactttc tgcaccgttt ccatcgggag
     1861 cactacttcg ttcaaggggc cttccgtccc ggttgctatc tggaatttct gattgaagat
     1921 ttgcgctata ccatcgtcac cgtacttgat ctccgttatg gccgatccat ccgtcgcggt
     1981 tttcagcgca tcgatcttta ccttgaggtc gttgagcgcc gagagatggt cgtcatcaaa
     2041 acccgggacg accatcttgt cgaggagtcc tttccaatac tccggatcgg tgatctctat
     2101 caagtttgcg gcatccgcct ggtaggcatc gagttgggcg agcgccggcg ccaagagttg
     2161 ctggagacta ccttcgaccg atcccttgag atgaccgaaa ttgaacgacg ggaaactcac
     2221 taccgcacgg aaaccggcat cttcagagat aacacttaaa ttaaactcca gttggttagg
     2281 atcaagcagg ttggtgtcga actctgtttt ataaactggc ggaaggatca cttgatcgaa
     2341 attgccgatg ttcccccatg ccttactcag atcgggggcc tgactgtttg caagcgaaga
     2401 gatacccgcc ctaccgaggg ccgtaagaag aatattgagg gtgtcggtaa agggaagatc
     2461 tgtcaactcc tctctcaatt ctgcctgcgc cggcacgctg agagaagata ccgccaaaga
     2521 atccgtgact gaagttacct tccctgtgca atcgcttggc ggaagacagt cctctgtggg
     2581 tctttgctca agagaaactc ctcccgtatc aagactccaa ccgctcaaca atatcgaccg
     2641 taactgttgg ggcgtaacca ttccgctgga aagccggtaa atgtcgagca catcggccat
     2701 tgattccttg aagaaatctt tcatcccctg atcgtcttga taactatcca taagaaccat
     2761 caactggttc atgatctcgc cgagatcagg cagttttatc tccgtaccca actgactact
     2821 attcccatac tttccgaaca tatcgagcgt gccgaggaaa tcgttaatgt cgcatccttt
     2881 ctccgatgac gtcgcacatt cttctgcttt ctctatcagt tttcccagta gttttttccc
     2941 ttgctcccgc gcaaaggtct tcagggtttc ggcgaaggta ttagtggtgc cgaaaacatc
     3001 gaactctgtt ctcagtgccg gcgtctctat cggcgaaata gaacttccct cttccccata
     3061 caccttcaat ggtgtatatt ccccattgac cgcatcgaga atacgggtga tgagtcgctc
     3121 gctcctccat gagatgtagg cgatcttggc gatgaaatcg ctgagaacga aacggagttt
     3181 cattacatcg ggatcggccc ggttcttaag gatcggtatc gaatcgagga atgccgcatc
     3241 gatatacccg gccgtcaaag ccatgaccga tgtctcggga gaagcctgaa tatccacggc
     3301 ggttccacgc accgatgccg attcgaagat gcctttgact gcatccatca ggttgagttg
     3361 tatggcgccc gccggtaaac tcattacggg accggcatcc atgacggtcg atccgcattt
     3421 caattgaggt tgagagccgc cggtgaactt tggcgcacag gctagggcgc tcttggtctg
     3481 catgagggtg gtgatgatct gccgaagcat gacggcgctc tcgttgggca ccttgagcgt
     3541 ctgcatcgtg taatcgagag tggaactcaa tgccgcctgt ctgagaagcc gcttgtagtc
     3601 aaggaccata ttcacggctt ctgagggaga agagaaatac tccttgatgg tcttcgtcgc
     3661 tccattttgc gttattttta cctttcccag taactcattg ggcggtatgt cttttatagc
     3721 atcgataaca tcttccctgc actcatcggg atattctttc aaacacggta gggactcacc
     3781 atcaatcttg aaggtgatgt cggtttccaa tgaaccgctg aatatttgac cgaggaacat
     3841 ctccaagacc gaagcgccga tgagatcctc tgtaagaact ttggcagggt tgccgctact
     3901 ggccaaccga cggatgaaat cgtagaaatt ctttccggcg ccgtatttcg actttatctc
     3961 gttgcgaatg atgttgacat tcccctgcat cgtggtttcg tagccgcgga ccaccgcttc
     4021 gatctgttgg cgctgttgtt cgagttttaa cagagcttct tgtgctttgg cttgcaaata
     4081 agattcatcc acatttggta atgcagatat ttccggcatg ttgttcattt ccgcttctat
     4141 cgagaaattc gcaccggtct tcaggatatc gaggatgtcg gccgggttgg tgatgttcgt
     4201 tccggcggtc aaagaaccat cggcgtaggc gcgggtgatg atctccttca tcagttcatc
     4261 ggcgacgatc tccagtatct tttcgtcgtc tatgagattg cccagccctt tgcctttgac
     4321 caatcctttc aacttcacca ggtcgaattt ctgtacctgc ttcatcgcat tggtgtacat
     4381 ctgttcgagt ttcttcatgt tcttgagatt gttgtacatc cactgcccga gttgcagaga
     4441 ggcgagttcg accagttgcg gattatcttg aagagcaatg tccaacgcag acttgttgac
     4501 atgtttcagc agtgccttgc ccagatgcgg caggaacgca tggaagtcgg tcatgtaggg
     4561 catcgggttg tacttgttcg gatcgagaat gagattcttg atcccggcgc cggcgatata
     4621 ggtgaagttg gcgttaaggt catcgacgcc gaactgctgg tagtcgctga accatttttc
     4681 gagattgctc ggtatcgcga acggattgcg cggcttctct tcctcccctt ctgccgtttc
     4741 ttgcggcgcc gccggattga gaagaccgcc cgctttcagt ttgcgtgccg ccatttcggc
     4801 ctgtttcagt ttatattcgg cctctatgat ggccatcttg tcacgcacgg cctgtatttc
     4861 ttcaagatac gcagaataag tctcttccca catatcgaaa cactgccatg tggttggttg
     4921 agcgacagaa ccactgatat ttctacaggc tggatgtgta gaaatgcttc ccagagacgc
     4981 caagacatca agcgatcttt tgttgtgatc ttcataagag ccgaagcacg aagtgaaggc
     5041 gtttttgccc acgagcaacg gatcctcttt tcttactatt tcctgacgaa gatattctcc
     5101 ctccacctcc tcgaaggcga aggttgtgtt cccgaaactg aaatagtctt ctatctcttg
     5161 gactttcgct accgcgtcct tgttcttgta actgcgttcc cattcctgat attcgcgaag
     5221 agcattgccc aagcgcaaaa cgacattctc cagatcgttg tcgttgttca ggtcgtcaag
     5281 gatcgagttc caaccctgag acggactgaa tacccaaagg ttggcggtgt tcctcaaata
     5341 cccgccctca tattttcccg tcacctcgct tagactgcca aaggcgttcg catcgttgtt
     5401 ggtcgccgag aggcattcct gataggtctt ttcgagcgtt tcgggaggaa gcagattgac
     5461 cgcctgcttc aggaagaacg acggttcgct gtcgcacaga tacaagattg cgggatttat
     5521 gttctgatca aagcagtaaa aatcgccgac cgcggtattg tatccgggaa gcgcaagcat
     5581 ttgcgggccg acggccgcca cctgaagtat cttttctatc ttgtggtcga tctgccggag
     5641 tacggtgtcg atcagatact cggtctcgcc attggggtac agttcggccc gttctttctg
     5701 cgtgagactt gctcgttttt cggcgttcat ctcgtcaaga taataccgcg tgcggggata
     5761 atggtcgcgc cacagtttct gcgcttccgg ggaatcctcg taacggctca tcctgatatc
     5821 ggtctccaat ttcgcctgca attcattgaa gcgttgaatt cgcgcctttt ttatcgcgat
     5881 ctcttgggga agtttttctt ttgtatactc cctcaagatg gccggatcgg cgatgtcggc
     5941 gatggcctga atatcgaaat aatcgcgata gaccgaaagt tgcgccaaca aaggcttcgt
     6001 cttgcgggct atgtcgtgga cgccatacaa cgattcgtcg gcggggttca tgcgcaaagc
     6061 ctcttccttg cgctcttgcc aaagttcttc gatgttggcg cgcgcaagta aaagatcgag
     6121 caactgttct ttcagaagag agatcgtttt cactttatat tcgtagacag aaagggtttc
     6181 atcgtcaatc tccgggcgtc cgaaaagcat acggtaatta gaaagactgt ggctcgccga
     6241 tttctcgccg caaagggcgt tcgagagata attctcgttc gggtccggga cctgacaaag
     6301 cgtctctccc gagaagaacg gtgttatctg tgtcgcgaaa ccgctgacat ccactccgcc
     6361 ggccattggc acattcggat cgtattgtaa gaaaagtccg ttcttcagat gcgcgccgtt
     6421 ctcttcggtc gtttcggcgg tttttgacat gtagaagacc acttcttgga atagattacg
     6481 ggagcgccgg attcctcttt tgatcggcgc attaagaagc aattccatct gatcgccggc
     6541 ccggtattga gtatttacca gcgaattaag atcgtgcatc gccaaggggg catataacgg
     6601 caatctggcg aatatttcgc ggatgcccct gatcggttgt cgttcatata tggcggccag
     6661 acgcatggca ttgacgatgt cgatatactt cttggtcgcg taatcgcggc aatcggtcag
     6721 tttctcattg cagtaaccga ccgggctttc cgttatcgcg taagacaaga tattgtccgt
     6781 cactgtatca ttaataacct tggctattgc cgattggtag gaagcggcgg accgaccgct
     6841 ccgtctggta acggtgaagg tactttcatc gctttgaagc ggcagggagt aataagagaa
     6901 ttgctgttcg ccgccggaac cgcaatctat gttatacaac cagcagttcg taatactgta
     6961 atcctcggta ctccccgttt ccgactcgaa atcgtcttcg cttccccggc caccggtcgg
     7021 gaaagaaccc agataggcca attgggtatc gttcccgccg tctatttcat cggtatactt
     7081 catgtcctgt cccggatcgg cgccgtcggg taacttgggg acattgaacc gaacatagtt
     7141 attgatggtc gcctgctcgt tctggagaca gccgatgaaa gtggcgcaat ccctgttgaa
     7201 cttgaggacc gggtcgggaa gacggcatat ccgttctatc tggagagttc ccgtttcctc
     7261 attgatggaa aggtcgtcct cttcaagata gtaacagcca ccttcatcgt ctcccaagag
     7321 ttccatttct tcggcgaggt ctttcggcac acagatcgcc ttggattggt ctttggaact
     7381 taccaccgcg cactgatcgg gatcgggttt taatgctccg gaatcgcagg ctatcgctgt
     7441 acagacgcgc tcaagcgtgg aaagatcggg cgttgtcccc gtttccaatt tgttgcggca
     7501 ggtgtaggtg cagttctcat cgaagcaacc gcaatcctca tccgttttgc aggtgtattt
     7561 ggtctgttcg atgcggcagg aggaaaccgg cgccttgcaa tcactattgg atgccgaaga
     7621 gaaacgacac ccgagatagg gttccgtgcc ggtgagtcct tttctcgtgc agaatccggt
     7681 gccgctacct ttgaattcgc aaatgccttc atatttggga tcacagatct tcaacagttt
     7741 gccgttgccg tccttctgcg ggatacaaca tctgcccccc acatagagac agaactgcat
     7801 catatcgcgg caaacgcccc ggaagagtat gccacaacgg ctccgctgat actgttcgct
     7861 gaaacaactc tcgaccgtgg ggtaactgca cccttcggtc tgcatctgag acaaaggtac
     7921 aatatcctcg tccggcgagg aagactggtc gacatcgggc gcacttgccg tatcgatatc
     7981 catcctttct tcgatatagg tcgaattgtt ctcgcgttcg cctgaatttt cctgggccgc
     8041 ttcggcgctg gtctctcccg ttgaatggat cgactctttg atgcaacgat cgcccaaaga
     8101 ataatagccc gcgttgcaaa gacagaaatc ggcgccatcc aaagaatggg caacgatacc
     8161 gttggtacat atcagatcgc tgtctatgtc cggttgcggg acgatatctt catcgctgac
     8221 gatatcgtcg tcggtccagc cgcattcgga ggtgtcccat gtacagaaaa gcgaggcctc
     8281 ggggggaagg cagtacgcat agccgatatt gtattgcgtg ccgttcaatg cggcacaggc
     8341 aatgtattcc gtatcgcact gctcgcttcc ttccctgata ccgttgccac agaccgcggt
     8401 cgctgtctgc acgccattgc agacggcgtt catgctgtca tctcccgtca aaaaggcaac
     8461 ctgtccggta tgttcggcga tcctatcctc atctatgacg agtaagccgc tctcctcttt
     8521 tactatgcag tgctgagcga taccgagttc catgactttc gtcagataat ccagagattc
     8581 tatgtcgtac agtccggtac cgttgatgaa aaggctctga acctccggcg tggcagcgag
     8641 ccactgaagc gttcttacct gtgtagcgga aatatcaaga taatccaatc tcccgaaatg
     8701 gtcgatgtcc caatcgtcgg cgacagcttc ggtcatagcg gtcccggaaa gatcgagata
     8761 acgaagattc tgcatgtcgc gcaggaaagt gaatgagttt atgtaggtgg tcccgctcag
     8821 atcaagatac tcgatgtcgt actgttcatc cagaacatcg tccgagaaag aagcctgaga
     8881 gataacatta tgggaagcat cgagttcccg caagcgaagg ttctttaaat cgggaaatag
     8941 cggagcgtat tccacttcca agggatatac tccggaaatg tcgagatatg ccagtgtccg
     9001 gggaagacgc gggagaaccg tgtataacag atcgttactg atatcgcaac cgtaggtgtt
     9061 ggcggccgtc tgacgatctt ccttgtttcg attgtcgctc acattttcga gatagaggcc
     9121 ctttaatctc ttaaatttat cgatcccatc gtatctgcca atgaatttgt accacggtcg
     9181 attgctgcag tggtggacga catctccgtg ggtaccgttg ggcagatcgg ggtattgcga
     9241 caatgtctgc gccacttcta ttgagcgatt gtccagcggg ttgtcgctga ggtcgagata
     9301 ttccagagcg ggccattgct ccgcaaggtc actttcgaat ttcttgacgc cattacactc
     9361 gccgctatag atgtcgcatt tacaggtatc cgtcagcaaa ccgctgatcg gtatttcgtt
     9421 tttctcgcaa agagtgccgt taccgatacg aaaaacaaga tccttgatcg ataccgattt
     9481 tatgtaagcg gggatccacg atgaaatcgg accttcgaat atattcttcg agttcttgat
     9541 ggaatctatt tcaaatacta tggaatcgta tggaccggac tgggcaactt caaaagcgcc
     9601 actcgccgtc acgatgccgc gtccatcatc ggtcgccgtt ccccctgtca gatttattga
     9661 gtccacaatc ggcgttattt tccccgagag aaaatacttc agatcaagtt taagatttct
     9721 cggagaaata tcgctgacga ggataccgtc gtcgaaaatc ggggagcgtc cgttgggatc
     9781 attataatcg tttatgaatc gttccagcat ctttttgtca tactgaacct ctatggtata
     9841 ggcagcaatc aatttctctc cactgacgcc cacccgggga aaagatgctt gatatctata
     9901 cttgttagcg gttttcccat caataaccgt ttttacctga aaggtgccgg ccggagaaga
     9961 gctgtactga tcttcacaaa aggcatcgca tcgaggcaac acctggtacg aactgtcatc
    10021 tgagccatat ctataggtgg ccgtggggaa ctcgccttcc ctgtttactc gacatacgac
    10081 attttcctct aactgttcaa gtgacggctt ttcgtctcct tgattgataa ccaatttgta
    10141 gtatactttt tcgggtacgc ttccgcattt ttcggtttgt aacaactcgc aatattgatc
    10201 ggtaatatcc aattccggat gtgaatcgtc gacttggtca ccagtataaa caacatccca
    10261 tatattgctg gggtttgtta ttatgcgatg ctggcaagca tcctccacct gtttttgcgc
    10321 ttcctcgacc attttatcga aattgcacgc gccacaagaa cagtatcccg ccgtaaaggc
    10381 atgaaaaata gtccccacca cgacgatttc tttatccgat gaaccttcgc atctggatat
    10441 tcgctgtatc tctatatttt ccgttagtcc tttcttcaaa accgccgagg agttttccgc
    10501 tttcaacggc ttcggcatct tgaaatcctt gtgttctcca caagcgcatt gacggtcgag
    10561 ggtatcgagg cgctgattga aatatttgat tccggtatcc cgcgcgatga atgcttgtaa
    10621 atcgtgcata tgccgaagtt cacgcagttc gccgtaccga cgctgacacg gttcgtcggt
    10681 ccagttctca taattacggc agttaacatc gttctcgttt accggcggtt cgagttgtag
    10741 catattatcg ctcaaatcga gccacttcat ttttcgcgta tgaataagcg gggacaactc
    10801 tcttccctgt tcacccccgt taaccagatg ctggggggag cccgccaaat tcagatgggt
    10861 cagattgggg tgataggcta tggcggacaa atcgtgaatg gaccgatgcg ttaaagatcc
    10921 tccacccaaa ttaaagatat gataattgcg actgagatga agcgacttca acccggacgg
    10981 cgtggtattt accactcgca ccgaatataa aagaccttct tctctatttg cacgaacaac
    11041 cggtatctct cccgttctct cgtcggcgca gaagattttt gtgtaaggag tgttggcaga
    11101 ttggaaatcg gttgtcgttt cacctttgtc ggcgataatg gcaggcgccc aaagcgataa
    11161 ttttaaggct ctttcaccgg taccacatcc cgcctcgctc aattcagttt cgttttcccg
    11221 ccaattgaga atgctttctt tatacccgaa ggtcggagtc ccattctcca ctttggtgac
    11281 cagcgcaaat tccgcgcgga gtttcgattc ctccaaggta tgcgaaaga
//
LOCUS       DAOQUE010000024        11295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_6479_length_11295_cov_4.394484, whole genome shotgun sequence.
ACCESSION   DAOQUE010000024 DAOQUE010000000
VERSION     DAOQUE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6479_length_11295_cov_4.394484"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PKH10_01445"
     CDS             complement(<1..525)
                     /locus_tag="PKH10_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT26818.1"
                     /translation="MTKKAVATARPDGRKEKGRKIREQILTATLELAGEKGSDALTAR
                     NLAERVGISKANLYHHFKNMAEIRAAACIHFLDILKPPSFEAPYTDPRKFLAELMTGV
                     GDFLRSNQKLHLGYGIIFSNEARNNTAFVRAIEKKVMGRKAFVREKLRAMMGLPTDDP
                     EIERILLALDIMREG"
     gene            692..2212
                     /locus_tag="PKH10_01450"
     CDS             692..2212
                     /locus_tag="PKH10_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26819.1"
                     /translation="MKRKYGMMIGALTAFMFCIGCGEGADPATDDTAPADNDTATLDE
                     DTLVPDVDTATLTKPGDDFNTPERTVLFEFKGLINSYEDVMAQTDTKGVMDLTLKLGD
                     IDIPMNDYPTAYRLLYPDEEQYGELAGTEHLIFGAHGDLKQEGSYVLYYTIIVGMPVT
                     QLEEIKKNKTPDATAETLEARTNLLHTTYLNRADNSIVWRRCMIAVLDKPEESRLFVN
                     FANNNDFTPGENILAWGNIALTTDFDPTKLTGYEEYEGQWCTFKLNDTVITKEQYEEE
                     LVKDVTKLDCDLPEGFLDGAGDTSATYTFKGVINEAGTQQNTAGYGTFSVKVAGTEIP
                     VSDYIAVAGTSTGTAGQELVSVESLGGLETITGQNHYKYNYFAAIVDKQTLLDAKEKG
                     DASCLFSGIGDGEKAFVQLFAVEEKQVNSVTYQKVCPTAVLAPLPATSAILYCIGGNT
                     AFAVGEEIQSAANVTLTTDEVYMMEIFEVDSPDKLCGCNENYTTVIECSAFDAVEP"
     gene            2224..3702
                     /locus_tag="PKH10_01455"
     CDS             2224..3702
                     /locus_tag="PKH10_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNT26820.1"
                     /translation="MERVIMLLAALLMALPLTAVEPDKGLIIVTTDYIKNGSAVLGDL
                     VAEKEKRGFTVRVVTESDYGGAGEKGAIRAAKIRDWLAANRAGYGFVLLIGDPSPKLG
                     DVPMVLGQPQGEGVSDPCGDTGYSCVKIPTDLYYGDLSGSWDLNEDGILAQLPVDMGE
                     GGIDFGSELAVGRIPVYFGDLEELDELLTHIVGYMQMAEKATAYRKKMLFPLSFVWFD
                     GYKVFHTNHENKETSETAEWFIHNILPSHSGVTYTRLYEAEGHYPTQFDYEIPLTQEN
                     LIGEWKKGYGMVLWGGHGMPTSVVRTVWTEDVNGNELGENDEVESPLLIGSNDGKEIA
                     SGQPSFIVAISCLVGFVSEPGSITHRFMVDGAAVGVISSSSVTDPSAMKWLELDAPLN
                     TTTLGEDNTAVLFFDRLIGGGYAGRVFADIRDELGDETVFRVIDHKLMLNYFGDPSLT
                     LYDHAVAAETPDTDDTVDDSPASDDTAPADDTSGGCGLVRLW"
     gene            3802..5304
                     /locus_tag="PKH10_01460"
     CDS             3802..5304
                     /locus_tag="PKH10_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HNT26821.1"
                     /translation="MVRTTLAVILFLFSTMLGAFEQPGVNGLNEIEQWLMERGIPVLS
                     LSEKVPMITNGTETGYTQFRGVVGLVMTVTQGGVTGQGACTATLIDPEVLLTAGHCVY
                     LRENYQLVLDGPADPSKVVVRNGANMFAGDPLANAQTIKKHETWTGNISSWGENVDMA
                     VIHLDRKIYHLAPYGIRNDPAEVVGDEGKIVGYGITASDAFDSGIHRMGDSKILKLKP
                     YGKNILEIGNPSGSCQGDSGGPFLTTQNDAYVVSGITSFGGQTCYPTYGNYYTYVLPY
                     RDWINAAVEEFTGHGLIETLACGDDDDECGPGDELDCATLGEEYVPGIMAPCHEGCWG
                     YDLTVCQEKPPVCGDEKVKSPEICDSNTIDCASLGQYKSGTAAPCNDTCDGWDDSGCE
                     ETVCGDGKKEGNEFCDGNATDCSTLGDYAEGTEAPCKDDCTYFDTTPCQPPVTDTAIP
                     DGNDMNPDGTPVTDETAVADTEENPDSVVVPDTGTAPRSGGSGGCALTMI"
     gene            complement(5301..5699)
                     /locus_tag="PKH10_01465"
     CDS             complement(5301..5699)
                     /locus_tag="PKH10_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HNT26822.1"
                     /translation="METKSFDTILERLRTLDIPEQFDLVVAVARGGLVPAALVAHRIG
                     CDLATIWLNFRDETHRPCRECPALMRPISFPYRGKRVLIVDDRSRTGATLNAAKELLG
                     EAALVRTLVVNGKADYSLFDEPCFKMPWMI"
     gene            complement(5699..6745)
                     /locus_tag="PKH10_01470"
     CDS             complement(5699..6745)
                     /locus_tag="PKH10_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT26823.1"
                     /translation="MPANEKAPAAAAPATVYFTRDLSPAGLMKAFEKVRPAVTGKRIG
                     LKVHFGEDGNAYYIKPPLMKDLALSLKATFVETNVLYPGKRRYTESHIALAKEHGFTY
                     APIDILDSEKTIEYPAKGLKHIKKAIVGSHMVNYDTIVVLSHFKGHMGAGFGGAIKNV
                     AMGLGAIAGKMAMHASTIPTVNTKKCTKCGLCVPQCAGNAITIEPKVVIDPEKCIGCA
                     KCIGVCPTRVYGVPWGSTDTPAFMEKLVEYAKIISDNKHMVYINVLADISPDCDCMAG
                     AQKPFTKDIGILVSTDMLAIDAAAYDLVNAATHTKDAFAKHSGVSGKPQLAYGEKIGL
                     GTTRYELIDLDRKK"
     gene            complement(6872..8524)
                     /locus_tag="PKH10_01475"
     CDS             complement(6872..8524)
                     /locus_tag="PKH10_01475"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNT26824.1"
                     /translation="MGKHLWGPLIILMLFCVSCGDGSPVTGTDPSIADEDAVTTDELL
                     ELDDDPLPDADTRFDLFIAALKKDLAESDAYGVSTAVIKDGAIIFAAAYGSKDPEGTT
                     PLTPDTLMQIGSTTKQMTAAGLLRKVEAGTLALDDTLEELLPSLEFARDASWDDALTP
                     HLLLSHQGGFYDWIPWNGPPDDTELADNTYGVYDELYYLMNPPGAFWNYSNPNFVLAG
                     LITETLDTRKWPDIMREDLFVPLGMDRTFLRKSEVEKDGDYALSFGLGIDDLQSGESG
                     PVTMEMMPDPGWGRPAGLVWTTPTQMLKWARFLMEGDPAVLADDLREKMVEEQVDTLY
                     GMGTVHYGYGIFVERGYLSEAGEWYEVPVWEHGGNTLSFTNILYVFPEQDFAVAICSS
                     AYHTDFSHSLDAAVTSLAGLPAPVIDAPVYTIDPARFDNHIGTYYDPNNVGMLIVTRE
                     GDQLLVEAPDLAGYGLSVTPQLTAISSDIFYLYIDGTPYDITFIRTGEEGPSTYLRQR
                     AFVATRVEDEMASRRAPSRAAIERWLSHARLDQIPLHLSPRP"
     gene            8737..10086
                     /locus_tag="PKH10_01480"
     CDS             8737..10086
                     /locus_tag="PKH10_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26825.1"
                     /translation="MKRTTWIAIMATFLLWACGDETEHKPATDTDQVVVTDNAVTDDT
                     VTDDTMTDDTVTDDTVTDDTVTDDTVTDDTVTDDTVTDEIPDTDGAGCTLGVPYSKNG
                     LNDIATDLSLTVYSNGWGTLLANLTMGTEGTITLDVPEEDPYNGDTPSYFIYETAVGF
                     FTEFTFAQFGDTITVDLDPIMPAQVNGHIFMAQTYFGPTALANTTVTVSHANGTAIGC
                     FTTDGDGRFVINLPAGGYQFAFTDMDSSMYDEYVETTGPGDYLDLLIPAQAQVDKPNI
                     YLYPTELIDISVTLGFPQGGFVTTSIPAYGGSWEVTVTPDGIIDGAYGYLFYEAQTPD
                     LSQYRNGWTVATAGLEQFFRGNLAAYGFAGREVEDFIDWWIPRLTDGPCYDVYPQTAV
                     EIAPFITLNIVPAPDSVQRLLYTVRATGDCAAHLDEPIIEPFIRDGFTVLEWGVVVK"
     gene            complement(10116..>11295)
                     /locus_tag="PKH10_01485"
     CDS             complement(10116..>11295)
                     /locus_tag="PKH10_01485"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNT26826.1"
                     /translation="GDGYTYCLGDCCDAPGDCPLPAAVHPGAYEVINGIDDDCDDEID
                     EKNDACDIGVTVSQDVASSALSLAKAAGFCEPWLLSATLGLAGPQATEYICDSGGLLT
                     CPKYNRLSLPFPYFDTTYQTYAVNPKFGSVLEKKQGDNIAILSTGPWNGPTKDASVAT
                     LAAGDMKTASTVPEDWINFQPDCTAPKAPSCGGAAPDPTTQNSCAGKPLPVVQDPIML
                     TVRLKVPPNAGAFRINTYFCSVEYPDTVCSASNYNDFFIALLDSTFNTVNPTSQHLNP
                     YDKNLAKDVLGNPVGVDLAPAGLFTICSEENNNWGFCTGDAELAGTGFDNFGGCGCTG
                     WLVTQGNVVPGEEITLRFAVFEQGTVSYGPDHSWDSTILLDNFEWLADETTPGTGIQE
                     "
BASE COUNT         2258 a         3335 c         3538 g         2164 t
ORIGIN      
        1 cccttcgcgc atgatgtcga gcgcgagcag tatccgttcg atctccgggt cgtccgtcgg
       61 caaccccatc atcgcgcgca gtttctcgcg gacgaaggcc ttgcgtccca tgaccttctt
      121 ttcgatggcc cgcacgaacg cggtgttatt gcgcgcttcg ttggagaaga tgatgccgta
      181 tcccagatgc aacttctgat tcgaccggag gaagtcgccg acccccgtca tgagttcggc
      241 gaggaacttg cgcggatcgg tgtagggggc ctcgaaggag gggggcttga ggatgtcgag
      301 gaaatggatg caggcggcgg cccgtatctc cgccatgttc ttgaagtggt ggtagaggtt
      361 cgccttcgag atgccgaccc gttcggcgag attgcgcgcc gtgagggcgt cggagccctt
      421 ttcgccggcg agttcgagcg tcgcggtaag gatctgttcc cgtatcttgc gccctttctc
      481 tttgcggccg tcgggccgcg ctgtcgcgac tgcttttttc gtcatccttc ggtccaccgc
      541 catcagtatg ccacgatagt tgccgatgta aagtttttgc gatcctcata atactctgaa
      601 attaaaagta aaaaaagtct tgcacgccgc ttgcatgtaa ctgaccgacc gttcggttag
      661 tttgtttttt gaccgtgtga tggaggcgac gatgaaaaga aagtatggca tgatgatcgg
      721 ggcgctgacg gcgttcatgt tctgcatcgg atgcggcgaa ggggctgatc cggcgaccga
      781 cgatacggcg ccggccgaca acgacaccgc gacgctcgac gaggatacac tcgttcccga
      841 tgtcgacacg gcgacgctca ccaagccggg cgacgatttc aacactcccg aacgcactgt
      901 gctgttcgag ttcaaggggc tcatcaactc gtacgaggat gtgatggcgc agaccgacac
      961 caaaggggtg atggatctca cgctcaaact tggcgacatc gacatcccga tgaacgacta
     1021 cccgaccgcc taccggctcc tgtatcccga cgaggagcaa tacggcgagc tggcggggac
     1081 cgagcatctc atcttcggcg cgcacggcga tctcaagcag gaggggagtt atgtcctcta
     1141 ctacacgatc atcgtcggga tgccggtgac gcaacttgaa gagatcaaaa agaacaagac
     1201 ccccgatgcg accgccgaaa cgcttgaggc gcggacgaac ctgctccaca ccacctatct
     1261 gaaccgcgcc gacaacagca tcgtatggcg ccgctgcatg atcgcggtgc ttgacaaacc
     1321 ggaggagtcg cggctcttcg tcaacttcgc gaacaacaac gatttcacgc cgggcgagaa
     1381 catcctcgcg tggggcaata tcgcactgac caccgacttc gatccgacaa aactcaccgg
     1441 ctacgaagaa tacgaggggc agtggtgcac cttcaaactc aacgatacgg tgataaccaa
     1501 ggaacagtat gaagaggagt tggtgaagga tgtgacgaag ctcgactgcg acctgcccga
     1561 aggattcctc gacggtgcgg gcgacacgag cgcgacctac accttcaaag gggtcatcaa
     1621 cgaagcggga acgcagcaga acaccgccgg ctacggcacc ttcagcgtga aggtcgccgg
     1681 gaccgagatc ccggtgagcg actacatcgc cgttgccgga acgagcaccg gcaccgcagg
     1741 acaggaactg gtgtcggtcg aatcgctcgg cggcctagag acgatcaccg gacagaatca
     1801 ttataaatac aattacttcg ccgccatcgt cgacaagcag acacttctcg acgcgaagga
     1861 aaagggcgat gcctcgtgtc tcttctcggg tatcggcgac ggcgaaaagg cgttcgtcca
     1921 actcttcgcg gtcgaagaga aacaggtcaa cagcgtcacc tatcagaagg tctgcccgac
     1981 ggcggtgctc gcgccgctcc cggccacctc ggcgatactc tactgcatcg gcggcaacac
     2041 cgccttcgcg gtcggcgagg agatccagtc ggcggccaat gtcacgctga cgaccgacga
     2101 agtatatatg atggagatct tcgaggtcga cagccccgac aaactctgcg ggtgcaacga
     2161 gaactacacg accgtgatcg agtgctcggc cttcgacgcg gtcgaaccgt gagggagggt
     2221 gtcatggaac gggtgatcat gctactcgcg gcgctcctga tggcgctgcc gctcacggct
     2281 gtcgaacccg acaagggact tatcatcgtg acgaccgact acatcaagaa cggctcggcg
     2341 gtgctcggcg acctcgtcgc cgaaaaagaa aagcgcggct ttacggtccg tgtcgtgacc
     2401 gagagcgact acggcggagc gggggaaaag ggcgctatcc gcgccgcgaa gatccgtgac
     2461 tggcttgccg cgaaccgcgc cggatacggc ttcgtcctcc tcatcggcga tccgtcgccg
     2521 aaactgggcg atgtgccgat ggtgctcggc cagccgcagg gcgagggggt ctccgacccg
     2581 tgcggcgaca ccggctactc ctgcgtgaag ataccgaccg atctctacta cggtgacctg
     2641 tcgggtagtt gggacctcaa cgaggatggc attctcgcgc agttgccggt cgatatgggt
     2701 gagggcggca tcgatttcgg atcggaactg gcggtcggtc gcatcccggt ctacttcggc
     2761 gatcttgagg aactcgacga attgctgacc catatcgtcg gttatatgca aatggcggag
     2821 aaggcaaccg cctaccgtaa gaagatgctc ttcccgctct cgttcgtctg gttcgacggc
     2881 tacaaggtct ttcacaccaa tcacgagaac aaggagacct ccgagacggc cgaatggttc
     2941 atccataata tcctgccgtc gcacagcggc gtcacctaca cgcggctcta cgaggccgag
     3001 ggacactatc cgacgcagtt cgactacgag atcccgttga cgcaggagaa cctcatcggc
     3061 gagtggaaaa agggctacgg catggtgctc tggggcggtc acgggatgcc gacgagcgtc
     3121 gtgcggaccg tctggacgga agacgtgaac ggcaacgaac tcggcgagaa cgacgaggtc
     3181 gaaagcccgc tgctcatcgg gtcgaacgac gggaaggaga tcgcctcggg tcagccgtcg
     3241 ttcatcgtcg cgatatcgtg cctcgtcggt tttgtgagcg aacccggctc gataacgcac
     3301 cgcttcatgg tcgacggcgc ggcggtcggc gtcatcagct cgtcgagcgt caccgatccc
     3361 tcggcgatga aatggctgga actcgatgcg ccgcttaaca ccacaacgct cggcgaggac
     3421 aacaccgcag tgctcttctt cgaccggctc atcggcggcg gctatgcggg gcgggtcttt
     3481 gccgacatcc gcgacgaact gggcgacgaa acggtctttc gcgtcatcga ccataaactg
     3541 atgctcaact atttcggcga tccgtcgctc accctctacg accacgccgt cgccgcagag
     3601 acgcccgaca ccgatgatac ggtcgacgac tcccccgcaa gcgacgatac cgcgcccgcc
     3661 gacgatacct ccggcgggtg cgggctcgtc cgactttggt agaaataacc atccgtaaga
     3721 actaacgaaa aaccgccgga cccgcacttg cttcttttcc tgtcagaggt aggataccgg
     3781 aacacggtca cggaggttga catggtacgg acgacactgg cggtgatatt atttcttttc
     3841 tcgacgatgc tcggcgcttt cgaacagccg ggtgtcaacg ggctcaacga gatcgaacag
     3901 tggctgatgg agcgtggcat cccggtccta tcgctctccg agaaggtgcc gatgataacg
     3961 aacggtaccg agaccggcta cacgcagttc cgcggggtcg ttggtctcgt gatgacggtg
     4021 acgcaggggg gcgtcaccgg gcagggggcc tgcaccgcga cgctcatcga ccccgaggtc
     4081 ctgctcacgg cgggacactg cgtctatctc agggagaact accagttggt gctcgacggt
     4141 ccggccgatc catcgaaggt cgtcgtccgc aacggcgcga acatgttcgc gggggatccg
     4201 ctcgccaacg cgcagaccat caagaagcat gaaacatgga ccggcaacat ctcttcgtgg
     4261 ggagagaatg tcgacatggc ggtcatccat ctcgaccgga agatatacca ccttgcgcct
     4321 tacggcatcc gcaacgaccc ggccgaggtg gtcggggacg aagggaagat cgtcggctac
     4381 ggcatcaccg cgagcgacgc gttcgattcg ggcatccacc gcatgggcga ctcgaagata
     4441 ctcaaactga aaccgtacgg gaagaacatc ctcgagatcg gcaatccgtc gggcagttgt
     4501 cagggcgatt cgggcgggcc gttcctcacg acccagaacg acgcctatgt cgtgtcgggc
     4561 atcacctcgt tcggcgggca gacctgctat ccgacctacg gcaactacta cacctatgtc
     4621 ctgccctacc gcgactggat aaatgcggcg gtggaagaat tcaccggcca cggacttatc
     4681 gagacgctcg cctgtggtga cgatgacgat gaatgcggtc cgggtgacga actcgactgc
     4741 gctacgctgg gcgaggagta tgtgccgggc atcatggctc cgtgccacga ggggtgctgg
     4801 ggctacgacc tcaccgtctg tcaggaaaag ccgccggtgt gcggcgacga gaaggtaaaa
     4861 tcgcccgaga tatgcgactc gaacacgata gactgcgcct cgctcggaca gtacaagtcg
     4921 gggacggcgg cgccgtgcaa cgacacctgt gacggatggg acgactcggg atgcgaggag
     4981 accgtctgcg gtgacgggaa gaaggaaggc aacgaattct gtgacggtaa cgcgaccgac
     5041 tgctcgacgc tcggcgacta tgccgaaggg accgaggcgc cctgcaaaga tgattgtacc
     5101 tatttcgaca ccacgccgtg ccagccgccg gtcaccgaca ccgcgatacc ggacggcaat
     5161 gatatgaatc ccgacggaac gccggtcacc gacgagacgg cggtcgccga taccgaagag
     5221 aaccccgaca gcgtcgtcgt tcccgacacc gggaccgctc caaggtcggg cggctcgggc
     5281 ggctgtgccc tgacgatgat ctagatcatc cagggcatct tgaagcaggg ttcatcgaag
     5341 agcgaataat cggctttgcc gttgacgacg agcgttctga cgagcgccgc ctcccccagc
     5401 agttccttcg ccgcgttcag cgtcgcgccg gtccgcgacc ggtcatcgac gataagaacg
     5461 cgcttgccgc ggtaggggaa cgatatcggc cgcatcagcg ccggacattc gcggcacggc
     5521 cggtgcgtct cgtcgcggaa gttcagccag atcgtcgcga ggtcgcagcc gatccggtgg
     5581 gcgacgagcg ccgccggaac aaggccgccg cgggcgaccg ccaccacgag atcgaactgt
     5641 tcgggtatgt cgagcgtccg caggcgctca agaatggtgt cgaaggattt cgtttccatt
     5701 attttttcct gtccagatcg atgagttcgt aacgggtcgt gccgagcccg atcttctcgc
     5761 cgtatgccaa ctgtggtttc cccgaaacgc ccgaatgttt ggcgaaggcg tccttggtgt
     5821 gggtcgccgc attgaccaga tcgtatgccg cggcgtcgat ggcgagcatg tcggtcgaaa
     5881 cgaggatgcc gatgtccttc gtgaagggct tttgagcccc cgccatgcag tcgcagtcgg
     5941 gcgatatgtc ggcgagcaca ttgatgtaga ccatgtgctt gttgtcgctg atgatcttgg
     6001 cgtactcgac cagtttctcc atgaaggcgg gggtgtcggt cgatccccac gggacaccgt
     6061 agacccgcgt cgggcagacg ccgatgcact tggcgcagcc gatgcatttt tcggggtcga
     6121 tgaccacctt gggttcgatg gtgatggcgt tcccggcgca ctgcgggaca cagagcccgc
     6181 acttggtgca tttcttcgtg ttgacggtcg ggatggtcga tgcatgcatc gccatcttcc
     6241 cggcgatggc gccgagtccc atcgcgacat tctttatcgc gccgccgaat ccggccccca
     6301 tgtgtccctt gaagtgcgac agaacgacga tggtatcgta attcaccatg tgcgaaccga
     6361 cgatcgcctt cttgatgtgt ttgagccctt tggccgggta ttcgatggtc ttctcgctgt
     6421 cgaggatgtc gatgggggcg taggtgaagc cgtgttcctt ggccagcgcg atgtggctct
     6481 cggtgtagcg ccgtttcccg ggatagagga cattggtctc gacgaaggtc gccttgagcg
     6541 agagtgcgag atccttcatg agcggcggtt tgatgtagta ggcgttgcca tcctcgccga
     6601 agtggacctt gagaccgatc cgctttccgg tgacggcggg gcgcaccttc tcgaatgcct
     6661 tcatgaggcc cgccggggag aggtcgcggg tgaagtagac cgtggccggc gcggcggcgg
     6721 ccggtgcttt ttcgttcgcg ggcatggtgc ctcccgaaac aagtatgcac agaagcatga
     6781 tgagaaaacg accattcatg gggaggatcc tacttgagca tcgccatttt tgcaagcgtt
     6841 tccccgtctt ccttccgtcg ccgttcctgc ctcaaggtcg tggcgacaga tgcaacggta
     6901 tctggtcaag ccgcgcatgc gacagccagc gctcgatcgc cgcgcgggaa ggagcgcgac
     6961 gcgaggccat ttcatcttcg acacgggtcg cgacgaaggc gcgttgccgc aggtaggtcg
     7021 acggcccctc ctccccggta cggataaagg tgatgtcgta gggggtgccg tcgatgtata
     7081 agtaaaagat atcgcttgag atagcggtca gttgcggcgt cacgctcagg ccgtatcccg
     7141 caaggtcggg cgcctccacg agcagttgat ccccttcccg tgtaacgatg agcatgccga
     7201 cattgttcgg gtcgtaatag gtgccaatat gattgtcgaa tcgtgccggg tcgatggtat
     7261 ataccggagc gtcgatcacg ggagccggta gtccggcgag tgacgtgacc gctgcgtcga
     7321 gactgtgaga gaaatcggtg tgataggcac ttgaacagat ggcgaccgca aagtcctgtt
     7381 ccgggaacac atagaggatg ttggtgaacg acagcgtgtt gccgccgtgc tcccagaccg
     7441 gcacctcgta ccattcgcct gcctccgaca ggtagccgcg ttcgacgaag atcccgtagc
     7501 cgtaatggac cgtccccatg ccgtagagcg tatccacctg ttcttcgacc atcttttcgc
     7561 gcaggtcatc ggcgaggacc gcggggtccc cctccatgag gaaccgcgcc cacttcagca
     7621 tctgggtcgg cgtcgtccag acgagccccg ccgggcggcc ccagccgggg tcgggcatca
     7681 tctccatcgt caccggtccg ctttcgccgc tttgcaggtc gtcgatgccg agcccgaagg
     7741 agagtgcgta gtcgccatcc ttctcgacct cgctcttgcg caggaaggtc cgatccatcc
     7801 cgagcgggac gaagaggtcc tcgcgcatga tgtccggcca tttccgggtg tcgagcgttt
     7861 cggtgatgag gcccgcgaga acgaagttgg gattcgagta gttccagaag gcgcccggcg
     7921 ggttcatgag atagtacagt tcgtcataga cgccgtaggt gttgtcggcg agttcggtgt
     7981 cgtcgggcgg cccgttccac ggtatccagt cgtaaaagcc gccttgatgg ctgagcagaa
     8041 ggtgcggggt gagcgcgtcg tcccatgagg cgtcgcgcgc gaactcaagg gaggggagca
     8101 gttcctcaag cgtgtcatcg agcgcgagcg tccccgcctc gaccttgcgc agaagtcccg
     8161 cggcggtcat ctgtttggtg gtcgaaccga tctgcatgag cgtatcgggc gtcagcggcg
     8221 tcgttccctc cggatccttc gagccgtagg cggcggcgaa gatgatcgcg ccgtccttta
     8281 tgaccgcggt cgagacgccg tacgcgtcgc tttcggcaag atcctttttg agcgccgcga
     8341 tgaaaaggtc gaagcgtgtg tcggcgtcgg ggagcggatc gtcgtcgagt tcgagaagct
     8401 catcggtcgt gaccgcatcc tcgtcggcga tcgacgggtc ggtgccggtg acgggggagc
     8461 cgtcaccaca ggaaacacag aaaagcatca agataataag cggtccccaa agatgttttc
     8521 ccatcctgtt cctcctgtcc gatgtcatga cccgcgatga tcagacaaaa aaaggagcaa
     8581 gaagggagcc tttctttttt ttatagtgta tccgaacctt tttggcgtat aatgtctttt
     8641 gtccataaat aaatagggga ggagtaggac accaaagggc cggtcgaaga ggaaggttcg
     8701 ttcacttacg aatagcgaga aaggagggaa caacaaatga aaagaacaac ttggattgcc
     8761 atcatggcga cgttcctgct gtgggcctgc ggcgatgaga cggagcataa gccggcgacc
     8821 gacaccgatc aggtggtggt caccgacaat gccgtcaccg acgatacggt gaccgacgac
     8881 acgatgaccg acgacacggt gaccgacgat acggtgaccg acgacacggt gaccgatgat
     8941 acggtgaccg acgacacggt gaccgacgac acggtgaccg acgaaatacc cgacaccgat
     9001 ggcgcggggt gcaccttggg agtgccctat tccaagaacg gtctcaacga tatcgcgacc
     9061 gacctatcgc tcacggtcta ttcgaacggc tggggtactc tgctcgccaa cctcacgatg
     9121 gggaccgagg ggaccatcac cctcgatgtt cccgaggaag atccctataa cggggatacc
     9181 ccttcctatt tcatctacga gacggccgtc ggctttttca ccgagttcac cttcgcacag
     9241 ttcggtgaca cgataacggt cgatctcgac ccgatcatgc cggctcaggt gaacggccac
     9301 atcttcatgg cgcaaacata cttcgggccg acggcgctcg cgaacacgac cgtgaccgtc
     9361 tcccatgcga acggcacggc gatcggctgc ttcacgaccg atggcgacgg gcgcttcgtc
     9421 atcaacctgc cggcgggcgg ataccagttc gccttcaccg acatggatag ttcgatgtac
     9481 gacgagtatg tcgagaccac cggccccggc gattatctcg acctgctgat accggcgcag
     9541 gcgcaggtcg acaaaccgaa catctatctc tatccgactg aactcattga catttctgtt
     9601 acgctcggct tcccgcaggg cggttttgtg accacctcca taccggcata cggcggcagt
     9661 tgggaggtga cggtgacccc cgacggcatc atcgacggcg cgtacggcta tctcttctac
     9721 gaggcgcaga cccccgacct ctctcagtac cgcaacggat ggaccgtggc gacggccgga
     9781 ttggagcagt tcttccgtgg caacctcgcc gcctatgggt tcgcggggcg cgaggtagag
     9841 gatttcatcg attggtggat accgcgcctg accgatggcc cctgttacga tgtctatccc
     9901 cagaccgccg tagagatagc cccgttcatc acgctcaaca tcgttccggc gcccgacagc
     9961 gtccagcgtc tcctctacac ggtgcgtgcg accggcgact gtgcggcgca cctcgacgag
    10021 ccgatcatcg aaccctttat ccgcgacggg ttcaccgtcc tcgaatgggg agtcgtcgtc
    10081 aagtgaatcg cctgcgggtc acgcgctgaa cagcgttatt cctgtatccc ggtgccgggg
    10141 gtggtctcgt cggcgagcca ttcgaagttg tcgagcagta tcgtcgaatc ccacgagtgg
    10201 tcggggccgt aggaaacggt cccctgttcg aagaccgcga accgcagggt gatctcctcg
    10261 cccgggacga catttccctg cgtcaccagc cacccggtac agccgcaccc accgaaattg
    10321 tcgaagccgg tcccggccag ttcggcgtca ccggtgcaga aaccccagtt gttgttctcc
    10381 tcgctgcaga tggtgaacag gcccgccggc gcaaggtcga cgccgaccgg attgccgagc
    10441 acgtccttcg cgagattctt gtcgtagggg ttgagatgct gcgaggtggg gttgacggtg
    10501 ttgaaggtcg aatcgagaag tgcgatgaag aaatcattgt agttggaggc gctgcagacg
    10561 gtgtcgggat actcgaccga acagaaatag gtgttgatgc ggaaggcgcc cgcgttgggc
    10621 ggcaccttga gtcgcacggt cagcatgatc gggtcctgca cgaccggcaa gggtttcccg
    10681 gcacaggagt tttgcgtggt cgggtcgggt gccgcgccgc cgcacgaggg cgccttgggc
    10741 gcggtgcagt cgggttggaa gttgatccag tcctccggca cggtgctcgc cgtcttcatg
    10801 tcgcccgccg cgagcgtcgc gaccgatgcg tccttggtcg gcccgttcca cggcccggtg
    10861 gagaggatgg cgatattgtc gccctgtttc ttctcgagca cgctgccgaa cttcgggttg
    10921 accgcatagg tctgataggt cgtgtcgaag taggggaacg gcaggctcag acggttgtat
    10981 ttcggacaag ttaaaagacc gcccgaatcg cagatgtatt cggtcgcctg cggccccgcg
    11041 agtccgagcg tcgccgagag gagccacggt tcgcagaaac cggccgcctt ggcaagggac
    11101 agggcgctcg acgccacatc ctgactgacc gtcacgccga tgtcgcaggc gtcattcttc
    11161 tcgtcgatct catcgtcgca gtcgtcgtct atgccgttga tcacctcata ggcgccgggg
    11221 tgaacggcgg ccggtagtgg gcagtcgccc ggcgcatcac aacagtcccc gaggcagtag
    11281 gtatagccgt cgccg
//
LOCUS       DAOQUE010000025        11240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_6512_length_11240_cov_4.800805, whole genome shotgun sequence.
ACCESSION   DAOQUE010000025 DAOQUE010000000
VERSION     DAOQUE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11240
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6512_length_11240_cov_4.800805"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1102)
                     /locus_tag="PKH10_01490"
     CDS             complement(116..1102)
                     /locus_tag="PKH10_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNT26827.1"
                     /translation="MNVGFTYDLKSDYIKAGFTEEEAAEFDGIETIEGVEGALVSLGH
                     RVERIGTVKRLAELLVVGKRWDLVFNIAEGVQGIGREAQVPTLLDAYDIPYVFSDPMV
                     LALCLHKGMTKRVVRDGGVPTAKFHVVADECDIAEVRLEYPLFAKPVAEGTGKGIGAF
                     SKISDAKVLRDRCLDLLGKHRQPVLVEEYLPGRECTIGIVGTGRDARVVGVMEVVFKP
                     AAEKEAYGLHNKKNYKEIMEYVVPEKALYEACGEVALTAWRVLECRDGGRVDVRCDRH
                     GVPNFIEVNPLAGLHPIDSDLPILARLNGTDYRALIGMIMDSALRRIERERR"
     gene            complement(1112..2377)
                     /locus_tag="PKH10_01495"
     CDS             complement(1112..2377)
                     /locus_tag="PKH10_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNT26828.1"
                     /translation="MKKPVVSSEEEVPLPTNEAVPLSEASIFDILPPKQQPAPVVLEP
                     RALAFLREHFPAAEEKDWANWKWQLSNSITSLDKLSAIVNLSKDEMRALSGKGGVLPL
                     RITPHYASLLAKNDPRQPIRRSVIPVTAEHQHCFGEAADPLHEENDSPVSGIVHRYPD
                     RVLFLATGFCSAYCRYCTRSRMVGDANRMHQFSTKIWDEAIDYIRRTPTIRDVLISGG
                     DPLTMPDAKIEYLLSALRKIEHVEILRIGTKVPAVLPQRITPKLVNLLKKYHPLFVSV
                     HFTHPDEVTPEVAQACMRLADAGIPLGSQTVLLKGINDDVPTMARLFHQLLKIRVRPY
                     YLYQCDPIFGSGHFRTTVERGLDIIQGLRGHTSGYAIPQFVVDAPGGGGKTPLLPDYW
                     QGRDTEYVYMKNYEGNLYRYPNVQRPLEK"
     gene            complement(2698..4074)
                     /locus_tag="PKH10_01500"
     CDS             complement(2698..4074)
                     /locus_tag="PKH10_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNT26829.1"
                     /translation="MKNTLLMQELRDMLRGEHYDDLRTYCHSNTPQVIAGFLEALDPG
                     EIKEVFSIIDRETAAAIFHHFEDEVKDALQDIMSEQEFSELLFELPEEVQQELIEDLP
                     DDERKTVESYIDLYEKVEEEEKRKIEEDARKERQILIYDEDGEILENLTIYKSTPEGL
                     KELPAVEDGCWINAVMPFPEEVDYLVERFNIPHDFLASALDIDERARIEKDDNATLII
                     TRMPVYDETNKSLMYATMAVGIILVNGVIITVSPRRNLVLEEFIDGEVRHFNTLDREH
                     FIMQILLRATLLFLLYLKQMNNIAMVIQKHILEASQNRQLSKMLSLEKSLVYFVTSLK
                     SNEFMMTRLKRMGLIREDDLMDDLWEDLEIEHKQAVEMAQIYSHVISNMMGAFGSIIS
                     NNLNLVMRILTSFTIVLTIPVIFASFYGMNVPNFLEDNRYAFGGIFVLSLAVSGIVIY
                     FFRKRHWL"
     gene            complement(4176..4952)
                     /locus_tag="PKH10_01505"
     CDS             complement(4176..4952)
                     /locus_tag="PKH10_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HNT26830.1"
                     /translation="MYELFFVFFATATVMAVVLGCMMLMRRKVSHAVMEKHNDVAGFI
                     YSVIGVVYAVLLAFVVIVVWELYREADAKVQEEVRYMSSLFRDARVFPEPHHSAIQAE
                     IMNYTDLVIDEEWPLLRDGKADDKALDSLHRIFDLYTRIQPQNDYERVWYQETVEKLN
                     DFSDARNQRILAGRAAVPGFMWIILVIGGIIVIGFSFLFGTINTWAHMLMVALLAGVV
                     TLVLLLILALDHPYRGIILVGPEAFVEQKAHFEKYLSSQK"
     gene            complement(4955..7126)
                     /locus_tag="PKH10_01510"
     CDS             complement(4955..7126)
                     /locus_tag="PKH10_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNT26831.1"
                     /translation="MDAKTLAFTFSDLIQGYVTNFDWDKQTFGLRTSDGREFSVTLTA
                     NTYAEMVRNLDEAFIDCTSQIKDMLGKDRFLYVYGVFYPEKELSFEAKHIVFLGRNEG
                     EFRFEKQDWWIRQVRCLGDFYLKAQFDNGAPDFSKYRTELNLEGGKDGSFRQETDTIS
                     RLVYGFASAFLMTGDDRYLDAAEAGTRYLRDHMRNVDSSEDIAYWYHAIDINGDRERK
                     VFSSEFGDDLDAIPAYEQIYALAGPTQTYRVTGDPAIRRDIEMTVNLFDKYFIDRKRG
                     GYFSHIDPITFDPRAETLGRNRARKNWNSVGDHAPAYLINAYLATEDERLRRMLIYTA
                     DTIATRFPDYEHCAFVQEKFHEDWSHDTTWGWQQNRGVVGHNLKIAWNLTRIISLPGL
                     EKDKIATYTAFAGKIAELMPRHGGDAQRGGWYDVVERMLADGQKFHRYAWHDRKAWWQ
                     QEQGILAYLILAGTAGSPEHLKLARESSAFYNAWFLDHDSGGIYFNVLANGLPYLLGT
                     ERFKGSHSMSGYHSFELAYLAAVYSNLLITKQHLDLYFKPYPNGFADNILRVAPDILP
                     AGSVKIEKVWIDGKEYGEFDATALTVKLPVTNDRFKVQVRLVPTSTVEHFWITVDRDG
                     DVVRATLSGDLDHLAIHGFRKACAAIAAENPRKVAFVMKDLKSMVPEGVRTMIFEKQK
                     MAIDSDLYIVGANEEIRQLFDRDELSEEIVLLEKYDLSRMR"
     gene            complement(7136..9223)
                     /gene="glgX"
                     /locus_tag="PKH10_01515"
     CDS             complement(7136..9223)
                     /gene="glgX"
                     /locus_tag="PKH10_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNT26832.1"
                     /translation="MKRPEYFIEPGRPYPLGATVDDTGVNFSLFSENATAIELLLFAY
                     SKEDHPFLMVPLDPAVNRTNHFWHVYLRGLKPGVQYAYRISGPCEVCEGHRFNPNKVI
                     LDPYAKGTCSHRWERGAACHDGDNLSSAMRGVGIDPRGFDCGATRPPRTPMKDSVIYE
                     MHVAGFTRNPNSRVDRRGKFLGAIEKIPYLKELGVTAVELLPVFQFDDREILRTLPDG
                     TELRNYWGYSTVGYFSPENSYCVNPAEGEHLNEFREMVKAFHAAGIEVILDVVYNHTD
                     EGNHQGPVYSFKGIDNRVYYHLVPGDRRYYMDYTGCGNTVNCNHPVVQKMITDSLTFW
                     VTEMQVDGFRFDEGSILSRDEEGRPMRYAPVLWAIDLKERFADTKLIAEAWDAGGLYQ
                     IGTFPGYRWAEWNGRFRDDVRRFVKGDPGVVGAVASRIAGSSDLYQHERHTPLNSINF
                     VACHDGFTLYDLTAYNEKHNEANGEGNRDGIDDNLSWNSGVEGDTGDPEVLAFRKRRI
                     KNFAAILLLSQGVPMILMGDEVGKSQRGNNNAYCQDNELAWFDWELVEKNVDLFRFFK
                     EMIAFRKANAALRRGAFFDGSVNARGRRDIEWHGCRLGAPGWNDPASKVLAFTMGAFA
                     VGEPDIHVMMNMDPQALDFEVPPAVPGRRWCRFADTALSAPQDISEPGKETAVAAGDY
                     RVKPYSVVILIEQ"
     gene            complement(9220..9552)
                     /locus_tag="PKH10_01520"
     CDS             complement(9220..9552)
                     /locus_tag="PKH10_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26833.1"
                     /translation="MFRKIIDDYHTTLFLGIMLALLGIIESARNVLISVLGMPITSPL
                     ILLGLGVFNVILAFAFFIMGARNIEAAEEVAHTPRPAGNEKLLAERIAELETRLSRLE
                     RGAKDGVS"
     gene            complement(9661..10263)
                     /locus_tag="PKH10_01525"
     CDS             complement(9661..10263)
                     /locus_tag="PKH10_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNT26834.1"
                     /translation="MDLAGKKIAVLFERDYYENEIFYYQHRFAEEGMELHFLTRLWGQ
                     PSLTFVGHEYQIPMECRESFEGMSDDELRSYSAVIVPAGIVADRLRYTEDIKKLPPAC
                     EFMSRAFAEKTIIKGIICHGMWLMAPIAELVKGRRAVVHNNLLGDAKAYGIDYVDEDV
                     VVDGDLVTARTGGHCHLFARRIIELLRTGGAVVSDGRRRR"
     gene            10437..10565
                     /locus_tag="PKH10_01530"
     CDS             10437..10565
                     /locus_tag="PKH10_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26835.1"
                     /translation="MEMFDYILFTVVGVMFVYEIVEWARGRHNIFSKIHAWLFPKR"
     gene            10544..10942
                     /locus_tag="PKH10_01535"
     CDS             10544..10942
                     /locus_tag="PKH10_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26836.1"
                     /translation="MAVPEEIIVIEGLPDLAAMKMPLIVGIDKWCLETLLSPPYFWLA
                     EPFAGTHAAGFMKPGTPIRPDFPVLLLCALDDATPGTDLFFLPRVVFHASEWHRNAAI
                     AATRASDIVVVSGNIADTVKKDGVMLPFPR"
     gene            complement(10952..>11240)
                     /locus_tag="PKH10_01540"
     CDS             complement(10952..>11240)
                     /locus_tag="PKH10_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26837.1"
                     /translation="DGLIVEGYEDTEKLGELTLDATSAALLGEFSIPEGGGYVAKNGI
                     RGHSAANTYTLFTADLSAYAGKTVTISFLFVSDYITSHFGVAIDDVTITTE"
BASE COUNT         2290 a         3234 c         3219 g         2497 t
ORIGIN      
        1 cggccgagtt cgtcaagcgc gccggacacc gcgcgtatct ccgcgatgag gtcggcgtca
       61 tcgggacgcg cgtggggcgg gatcgggttg tggagcaggg cgatgagccg gtcgctcatc
      121 ggcgttcgcg ctcgatacgg cgcaacgcgg aatccatgat catgccgatg agagcgcggt
      181 aatcggtgcc gttcaatcgc gcgaggatgg gcagatccga atctatcggg tgcaggcccg
      241 caagcgggtt gacctcgatg aagttgggca cgccgtgacg gtcgcaccgc acatcgaccc
      301 gaccgccatc gcggcattcg agcacgcgcc acgccgtgag cgccacctcg ccgcaggcct
      361 cgtacagcgc cttctcggga acgacatatt ccatgatctc tttgtagttc ttcttgttgt
      421 gcagaccgta tgcctccttc tcggcggccg gcttgaacac gacctccatg acgccgacga
      481 cccgggcgtc gcggcccgtg ccgacgatgc cgatcgtgca ttcgcgcccg ggcaggtact
      541 cttcgacgag taccggctga cggtgttttc cgagcagatc gagacagcgg tcccgaagca
      601 ccttcgcatc ggatatcttc gagaaggcgc cgatcccttt gccggtacct tcggcgaccg
      661 gcttggcaaa gagcgggtat tcaagccgca cctcggcgat atcgcactcg tcggcgacga
      721 catggaattt cgcggtcggc acgcctccgt cgcgcaccac gcgtttggtc atccccttgt
      781 gcaggcagag cgcgagcacc atcggatcgg agaagacata ggggatgtcg taggcatcaa
      841 gtagcgtcgg gacctgcgct tcgcgcccga tgccctgcac cccttcagcg atgttgaaga
      901 cgaggtccca ccgcttgccg acaacgagca gttcggcaag tctttttacg gtgccgatcc
      961 gttcgacgcg atgaccgagc gacacgagcg ccccttcgac accctcgatc gtctcgatgc
     1021 cgtcgaactc ggccgcctct tcttcggtga aacccgcttt gatataatca ctcttgaggt
     1081 cgtaggtgaa cccgacattc atggatcgtg cttatttctc cagcgggcgc tggacattcg
     1141 gataccggta gaggttgcct tcgtaattct tcatgtagac atactccgtg tcgcggccct
     1201 gccagtagtc gggcaggagc ggggtcttac caccgccgcc gggcgcgtcc acgacgaact
     1261 gcggtatggc gtatcccgag gtgtgaccgc gaagcccctg tatgatatcg agtcctctct
     1321 cgaccgtcgt acggaagtga cccgacccga atatcggatc gcattggtac aggtaatagg
     1381 gacgcacccg tatcttgagc agttgatgga acaggcgcgc catcgtcggc acatcgtcat
     1441 tgatgccttt gaggagcacc gtctgcgaac cgagcgggat gccggcgtcg gcgagacgca
     1501 tgcaggcctg cgcgacctcc ggcgtcactt cgtcggggtg ggtgaagtga acgctaacga
     1561 agagcggatg gtatttttta agcaggttca ccagtttcgg cgtgatgcgt tgcggcagga
     1621 ccgcggggac cttcgtgccg atgcgcagta tctcgacatg ctcgatcttg cgaagcgcgg
     1681 acaggagata ctctatcttc gcgtcgggca tcgtgagcgg atcgccgccc gatatgagga
     1741 catcgcggat ggtgggggtt cgacggatgt aatcgatcgc ctcgtcccag atcttcgtcg
     1801 agaactggtg catgcggttc gcgtcgccga ccatgcggga gcgggtgcag taacgacaat
     1861 aggccgagca gaaaccggta gcgaggaaca ggacgcgatc gggataccgg tgcacgatgc
     1921 ccgataccgg actgtcgttc tcctcgtgca gcggatcagc cgcttcgccg aaacagtgct
     1981 ggtgctcggc ggtgaccggg atcacgctgc ggcggatcgg ctgacgcggg tcattcttcg
     2041 ccagaagact cgcgtaatgc ggcgtgatgc gaagcggcag gacccctcct ttacccgata
     2101 gagcgcgcat ctcgtccttg ctgagattca cgatggcgct gagtttgtcg agagaggtga
     2161 tgctgttgga cagttgccat ttccagttgg cccagtcttt ttcctcggcc gccgggaaat
     2221 gctcgcgcag gaaagcgagc gctcgcggct ccaagacgac cggcgcaggt tgctgtttcg
     2281 gaggaaggat gtcgaagatg gaggcctcgg aaagcgggac ggcctcgtta gtagggagcg
     2341 ggacctcttc ttcgctggaa accaccggtt tcttcatcgc cgtacccctc cgtggctagt
     2401 ggcggatgca acattcctat ttgtaaggat ttttttatct tttgcaattt ttcttctgtt
     2461 ttttgcaaaa atttttatac agtgtttaca gcgtattacg aaaaaaatgt ttgttttatc
     2521 aaaaaaatcg tcaatagcgg gaagttcgat tttttatcga acaacatttt tcacattttt
     2581 tcgggcggca agatctatgc cgtttttgac ctgttttcta ggcaccatga gataacaacc
     2641 ctattttcat gggggcattt ttttcttgtt ttacaaaaag cccttgttga aaaggggtca
     2701 cagccagtgc cgtttcctga aaaagtagat aacgatacct gacacggcga gtgaaaggac
     2761 aaagatcccg ccgaacgcat agcgattatc ttcgaggaag ttaggcacat tcatgccata
     2821 gaacgacgcg aatatgaccg ggatggtaag cactatcgtg aaggaggtga gtatgcgcat
     2881 gacgaggttg aggttgttgg agatgatgga gccgaaggcg cccatcatgt tgctgatgac
     2941 atgcgagtat atctgcgcca tttcgaccgc ctgtttatgt tcgatctcga gatcttccca
     3001 gagatcatcc atcaggtcgt cctcgcggat cagccccatc cgcttgagtc gcgtcatcat
     3061 gaattcgttc gatttgagcg aggtgacgaa atagacgagc gacttctcga gactcagcat
     3121 cttggagagt tgcctgttct gtgacgcttc gagtatatgt ttctgaatga ccatggcgat
     3181 gttgttcatc tgcttgaggt agagcaggaa cagcaacgtg gcacgcaaga gtatctgcat
     3241 gatgaaatgt tcgcgatcga gcgtgttgaa gtgacgtacc tcgccatcga tgaactcttc
     3301 aaggaccagg ttccggcggg gagagaccgt gatgatgacg ccgttcacga gtatgatgcc
     3361 gaccgccatg gtggcgtaca tgagactctt attcgtttcg tcgtataccg gcatgcgtgt
     3421 aatgatgagc gtggcgttgt catccttttc gatgcgcgcc ctctcgtcga tgtcgagcgc
     3481 cgaggcgagg aaatcgtgag gtatgttgaa acgctcgacg aggtaatcga cctcttcggg
     3541 gaacggcatc actgcattga tccagcaacc gtcctcgacg gcgggcaatt ccttcaaccc
     3601 ctccggtgtg gacttgtaga tcgtgaggtt ctcgagtatc tccccgtcct catcgtatat
     3661 cagtatctgc cgctcttttc tcgcgtcctc ctctatcttg cgcttttcct cttcctccac
     3721 tttctcataa aggtcgatgt acgactcgac cgttttgcgc tcgtcgtccg gcaggtcctc
     3781 gatgagttcc tgctggacct cttcgggcag ttcgaacagg agttcggaga attcctgttc
     3841 actcatgatg tcctgaaggg cgtccttgac ctcgtcctcg aaatggtgga atatcgccgc
     3901 cgccgtttcg cgatcgatga tagagaacac ctcttttatc tccccggggt caagcgcctc
     3961 gagaaagccg gcgatgacct gcggggtgtt cgagtgacag taggtgcgca ggtcatcgta
     4021 atgttcaccg cgaagcatgt cgcggagttc ttgcatcaga agggtgttct tcatggcggc
     4081 ctcccgcaat gaaggggtag tgacgaaccg acggccccat aatgcacaat cggtcaaaaa
     4141 aagcaacgaa attaagaaaa tggaaaagga tcagttcact tttgcgatga caggtatttt
     4201 tcgaaatgcg ctttctgttc cacgaaagcc tccggcccca ccagaatgat cccccgatag
     4261 gggtggtcga gagcaaggat cagcagcagc accagcgtga cgacgccggc gagcagggcg
     4321 accatgagca tatgggccca cgtatttatg gtaccgaaaa ggaagctgaa tccgatgacg
     4381 atgatgccgc cgatcacgag gatgatccac atgaatccgg ggaccgctgc tctgccggca
     4441 aggatccgct ggttgcgcgc atcgctgaag tcgttgagtt tctcgacggt ctcctgatac
     4501 cagacacgtt cgtaatcgtt ctgcggctgg atcctcgtgt acagatcgaa tatccgatgt
     4561 agcgaatcga gcgctttatc atccgcctta ccatcgcgca gaagcggcca ttcttcatcg
     4621 atcacgagat ccgtataatt catgatctcg gcctgtatcg ccgagtgatg cggctccggg
     4681 aagacccggg catcccggaa caacgaggac atgtatcgca cctcttcctg cactttcgcg
     4741 tcggcctcac ggtacagttc ccatacgacg atcaccacga acgcgagcag gacggcatag
     4801 acgacgccga taacagaata gataaatccg gcgacgtcgt tgtgtttctc catgaccgcg
     4861 tgactcacct ttctcctcat caacatcata cacccgagaa cgacggccat caccgtcgcg
     4921 gtggcgaaaa agacgaaaaa gagctcgtac atggtcacct catgcgggaa agatcgtatt
     4981 tttcaagcag cactatctcc tccgagagtt cgtcccgatc gaacagttgg cgtatctctt
     5041 cgttggcacc gacaatatac agatcggaat cgatcgccat cttctgtttc tcgaagatca
     5101 tggtgcggac gccttcgggc accatgctct tcagatcctt catgacgaac gccaccttgc
     5161 gcggattttc cgcggcgatg gcggcgcacg ctttgcggaa accgtgtatc gccagatgat
     5221 cgagatcgcc cgaaagggtc gcccggacga catcgccgtc gcgatcgacc gttatccaga
     5281 aatgttcgac cgtggaggtg gggacgagac gcacctgcac cttgaaccgg tcattcgtga
     5341 cgggcagttt caccgtcagc gccgtagcgt cgaattcgcc gtattccttg ccgtcgatcc
     5401 agaccttctc tatcttcacc gatccggcgg gaagaatgtc gggagcaacg cgcaggatat
     5461 tgtccgcgaa cccgttcgga tagggtttga aatagagatc gaggtgctgt ttcgtgatca
     5521 gaaggttgct gtagacagcc gccagatagg ccagttcgaa agaatgataa ccgctcatgc
     5581 tgtgactgcc cttgaagcgt tcggtcccga gcagataagg caggccgttg gcgagcacat
     5641 tgaaatagat gccacccgaa tcgtggtcga ggaaccacgc attataaaag gcgctcgatt
     5701 ctcgggcgag tttaagatgt tcggggctcc cggccgttcc ggcaaggatg agataagcaa
     5761 gtatcccctg ctcctgctgc caccaggcct tgcggtcatg ccatgcgtaa cggtggaact
     5821 tctgaccgtc tgcgagcatc cgttcgacca catcgtacca gccgccgcgc tgtgcgtcgc
     5881 cgccgtgtcg cggcatgagt tcggcgatct tacccgcgaa cgcggtatag gtcgcgatct
     5941 tatccttttc caggccgggc aaacttatga tgcgggtcaa attccacgcg atcttgaggt
     6001 tgtgccccac tacgccgcga ttctgctgcc aaccccatgt ggtgtcgtgc gaccagtcct
     6061 cgtggaactt ttcctgaacg aacgcgcagt gttcgtaatc ggggaaacgg gtcgcaatag
     6121 tgtcggcggt gtagatgagc atccgtcgca ggcgctcatc ctccgtggcg agataggcat
     6181 tgatgagata agccggcgcg tggtcgccga cgctgttcca gttcttccga gcgcggttcc
     6241 gaccgagcgt ttcggcccga ggatcgaagg tgatcggatc gatgtgcgag aagtagccgc
     6301 cccgtttgcg gtcgatgaaa tacttgtcga acaggttgac cgtcatctcg atgtcgcgac
     6361 ggatggccgg gtcgccggtc acccgatagg tctgggtcgg gccggccaga gcatagatct
     6421 gctcgtacgc agggatcgcg tcgagatcgt cgccgaattc gctggagaac actttccgtt
     6481 cgcggtcacc attgatatcg atggcgtgat accagtaggc gatatcttcc gacgagtcga
     6541 cattgcgcat atggtccctc agataacgcg ttcccgcttc ggccgcatcg aggtagcggt
     6601 cgtcacccgt catcaggaat gccgaggcga agccgtagac caatcgactg atggtatcgg
     6661 tctcttgccg aaaactgccg tccttgcccc cctccagatt gagttccgta cggtacttgc
     6721 tgaaatcggg agcgccgttg tcgaactgtg ccttcaggta gaaatcgccc agacagcgca
     6781 cctgccgtat ccaccaatcc tgtttctcga agcggaactc gccctcgttg cgcccgagga
     6841 acacgatatg tttcgcctca aaggagagtt ctttctcggg ataaaagacc ccgtacacat
     6901 acagaaaacg gtccttcccg agcatatcct ttatctggga ggtgcaatct atgaatgcct
     6961 cgtcgagatt gcgtaccatc tcggcataag tgttcgcggt gagcgtcacc gagaattccc
     7021 gaccgtcgga ggtccgcagg ccgaaggtct gcttgtccca gtcgaagttc gttacatacc
     7081 cctgaatgag gtcggagaac gtgaaggcga gcgttttggc atccatgact tcctcctatt
     7141 gttcgatcag aatgacgaca ctgtaaggct ttactcggta atcaccggcg gctaccgccg
     7201 tttccttccc cggttcagag atatcctggg gcgcggaaag cgcggtgtcg gcgaaacgac
     7261 accaccgtct gccgggaacg gcgggcggca cctcgaagtc gagggcctgc gggtccatgt
     7321 tcatcatgac gtgaatgtcg ggctccccca cggcgaaagc acccatcgtg aaggcgagca
     7381 ccttcgacgc gggatcgttc cagcccggcg ctccgagacg gcacccgtgc cactcgatat
     7441 cgcggcggcc ccgcgcgttg accgagccat cgaagaacgc gccccttcgc aacgcggcgt
     7501 tcgccttgcg gaaggcgatc atctctttga agaaacggaa caggtcgacg ttcttctcga
     7561 cgagttccca gtcgaaccac gctaattcgt tgtcctgaca gtaagcatta ttgttcccgc
     7621 gctggctttt ccccacctca tcgcccatga ggatcatcgg gactccctgc gacaggagca
     7681 gtatcgccgc gaaattcttg atgcgccgct tcctgaacgc gagcacttcc ggatcaccag
     7741 tatcaccctc cacaccggaa ttccaactca ggttgtcgtc gatgccgtcg cggtttcctt
     7801 cgccgttcgc ctcgttgtgt ttctcgttgt acgccgtgag atcgtacagc gtgaagccgt
     7861 cgtgacaggc gacgaagttg atcgagttga gcggcgtgtg ccgttcgtgc tgatacaggt
     7921 cgctcgaacc ggcgatgcgt gaggcgaccg ctccgacaac gcccggatcg cccttcacga
     7981 aacggcgcac atcgtcgcgg aatcgcccgt tccattcggc ccagcggtac ccgggaaagg
     8041 tcccgatctg gtagagaccg ccggcatccc acgcctcggc gatgagcttc gtgtcggcga
     8101 accgttcctt gagatctatc gcccagagca ccggcgcata acgcatcggc ctcccctcct
     8161 cgtcgcgcga aaggatggaa ccctcatcga agcggaaacc atccacctgc atctcggtca
     8221 cccagaaggt gagcgaatcg gtgatcattt tctgcaccac cggatggtta cagttgacgg
     8281 tattgccaca gccggtgtag tccatatagt agcggcggtc accggggacc agatggtaat
     8341 agacgcggtt gtcgataccc ttgaaagaat agaccggtcc ctgatggttc ccctcgtcgg
     8401 tgtgattata gaccacatca aggatcacct cgataccggc tgcgtgaaaa gccttgacca
     8461 tttcgcggaa ttcgttgagg tgttcgccct cagcgggatt gacgcaatag ctgttctcgg
     8521 gagagaagta gccgaccgtg ctgtagcccc agtaattcct gagttcggtg ccgtcgggca
     8581 gagttcgcag gatctcgcgg tcatcgaact ggaagaccgg cagcaattcc accgcggtca
     8641 ccccgagttc cttgagatac ggtatcttct ctatcgcgcc gaggaatttt ccgcgccggt
     8701 cgacccgcga attcgggttg cgcgtgaacc cggcgacatg catctcgtag atgacggaat
     8761 ccttcatcgg ggtccgcggc ggacgggtcg ctccgcagtc gaagccgcgc ggatctatcc
     8821 ccacgccgcg catcgccgaa gagagattgt cgccgtcatg acacgccgcg ccgcgctccc
     8881 agcgatgact gcaggtccct ttcgcgtagg gatcgaggat cactttattg gggttgaagc
     8941 ggtgcccttc acagacctcg cacgggccgg aaatacgata tgcgtactga acgcccggct
     9001 tcagaccgcg aagatagaca tgccagaagt ggttcgtacg gttgaccgcg ggatcgagcg
     9061 gtaccatcag aaaggggtgg tcctcttttg agtaggcgaa gagcaacagt tcgatcgccg
     9121 tggcgttctc ggaaaaaagc gagaagttca ccccggtatc gtcgaccgtc gccccgagag
     9181 gatagggtcg gccgggttcg ataaagtatt cgggtctttt catgatacac cgtccttcgc
     9241 cccccgctcc aggcgggaaa gtcgtgtttc gagttcggcg atccgttcgg caagcagctt
     9301 ctcgttaccg gcgggacgag gagtatgcgc gacctcttcg gccgcctcga tattgcgggc
     9361 ccccatgatg aaaaaggcga acgccagtat cacattgaac acgccgagtc ccaacaggat
     9421 aagaggcgag gttatcggca tgcccagcac tgagatcaga acattccggg cgctctcgat
     9481 gatcccgagt aacgcgagca tgatgccgag gaacagcgtc gtgtgataat catcgatgat
     9541 ctttctgaac atggtgttct cccttttcgt tctgtcgtat cttatgatcg caagttcttt
     9601 accttcgaca ccaaaagtat agttgccggt cgggggatca caccatggac caatgcagca
     9661 ttatcgcctc cggcggccat cggagacaac cgcgccaccc gttctcaaaa gttctatgat
     9721 ccggcgggcg aagagatgac agtgcccgcc ggtccgcgcc gtcaccagat cgccgtccac
     9781 cacgacatcc tcgtccacat agtcgatacc gtacgctttc gcatcgccca ataggttgtt
     9841 gtgaaccacc gcgcgacggc ccttcaccag ttcggcgatg ggggccatca gccacatgcc
     9901 gtgacagatg atccccttga tgatggtctt ctcggcgaac gcgcggctca tgaattcgca
     9961 cgccggcggc agtttcttta tgtcttcggt atagcgaagc cggtcggcga cgataccagc
    10021 cggcacgatg acggcgctgt aactgcgcag ttcgtcgtcc gacatcccct cgaaactctc
    10081 gcggcattcc atcggtatct ggtattcatg gccgacgaag gtgagcgacg gctggcccca
    10141 caggcgggtc aggaaatgga gttccatccc ctcctcggcg aaccggtgct gatagtagaa
    10201 tatctcgttc tcgtagtagt cgcgctcgaa caggaccgct attttcttcc ctgcaagatc
    10261 catcggcact ctccctaaag actcaccatc cgttcttcac gctgtagtta tatcacatta
    10321 gaaagcacac ctcaaggaaa gctgtagcgt tgttcttttc tttccctccc gccatttttc
    10381 ttcttggttt tttccactta ccggtatatg ctttgcttcc tcgcagaggg aacgacatgg
    10441 aaatgttcga ctacatactg ttcaccgtcg tcggcgtgat gttcgtctac gagatcgtcg
    10501 agtgggcccg tggtcggcac aacatcttct ccaagataca cgcatggctg ttcccgaaga
    10561 gataatagtg atcgagggct tgcccgacct cgcggcgatg aagatgccgc tcatcgtcgg
    10621 tatcgacaag tggtgtctcg aaacgctcct gtccccgccc tatttctggc tcgccgagcc
    10681 gttcgccggg acccatgccg ccggtttcat gaagccgggg accccgatca ggcccgattt
    10741 tcccgtgctg ctgctctgcg cgctcgacga tgcgacgccc gggaccgacc tcttttttct
    10801 gccgcgtgtc gtgttccacg cctccgaatg gcatcgcaac gccgccatcg cggcgacccg
    10861 cgcgagcgat atcgtcgtgg tgagcggcaa cattgccgac accgttaaga aagatggggt
    10921 gatgttaccg ttcccgcgct agacggaccg gtcactccgt cgttatcgtg acatcgtcta
    10981 tcgcgacgcc gaagtggctg gtgatgtagt cgctgacgaa caggaaactg atcgtcaccg
    11041 ttttgccggc ataggccgaa agatcggcgg tgaaaagcgt gtaggtgttc gcggcgctgt
    11101 ggccgcggat gccgttcttc gcgacataac caccaccctc cggtatgctg aattcgccga
    11161 ggagcgcggc actcgtggca tccagcgtga gttcgccgag tttctcggta tcctcatacc
    11221 cttcgacgat aaggccgtca
//
LOCUS       DAOQUE010000026        11052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_6650_length_11052_cov_5.359007, whole genome shotgun sequence.
ACCESSION   DAOQUE010000026 DAOQUE010000000
VERSION     DAOQUE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6650_length_11052_cov_5.359007"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..2786
                     /locus_tag="PKH10_01545"
     CDS             375..2786
                     /locus_tag="PKH10_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26838.1"
                     /translation="MNDLTKMAKNINYDSRRSFLLSMKETKLHEYIKELFVSMEPSYY
                     VEITHGSNEMGKDLVLIRSDKLSTEAIAVVVKKGTISGKTAGDIDEIRNDVQKIFSSG
                     ERKIYDEIISQIRQSMCNKAELKTLIDKVIISRVFVFLFGELSNQAKDRLDGELKAYE
                     GAVEYYPMKRIIDLFTDFYPQIFFKGKLHDYIMDKIKCLEDSVVIKTGKSKLSEIFVP
                     PLITKTGFPVFNSLEDAADYTKKQVLKTGKEKISLQNIDKLFSNNRKVILIGEPGSGK
                     SCALNKIALDAFISAQKDIVDGKSGSVSLPIKLHSKRLVNDNIIDNIVEDYIGKEALG
                     DNKITIKYILVDSLDEVDQEDREKVIENASKISDKYNAPLVITSRKLGMLDQPPADFE
                     KYEILPFSYVQVLKFMKNMFFDDLAKMNIIKKQINQLNMPYYPLALTLLVELVSEYNE
                     IPASITELYSRYFDLALGKEDRDKGISILFEYTIKRQFLAKLAYCEMMLKNQTSITKE
                     EFVVFCENYKSDIPSNHSKDMVAFVNEIERTGIIKCKDKVFFGHRSFMEYFSAYHVFE
                     NQTEYEDINQHLVDLYFNDKNEDIVFFFCGMKRKLSQKLVKKIFEEKPKDMDEIQSML
                     IKYMTPRLLQSAFSTNEQTKIETIINAANISGKIRNGIMSTFMPIKNKISPIFADIIM
                     MNYSMYSLSSMFIKNEIIEAIKVISKNTEPEVPYMMMYLLGGIEEKISIEEKDEIIKK
                     IIDTMINNKISKKDEAQILLFLIKMEENNKSIKSATRKLEKIKRRNKELFNTLFIPQK
                     IKKLL"
     gene            2826..4265
                     /locus_tag="PKH10_01550"
     CDS             2826..4265
                     /locus_tag="PKH10_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HNT26839.1"
                     /translation="MLYDLVGCPHRVTMDLFGNPADRDPVNAFVQLLWDRGAAHEDDV
                     IATLDIPPLDLSGLSSAEKEARTIEAMNRGVPLIYSGRITVDNLIGEPDLLRKEGPGY
                     IAGDIKSGAGEEGGDDGDDGKSKKHYAVQLALYTDILERLGRSAGRRAFVWDIARNEV
                     PYDLTGSLWDLYQSCLSQAQSITDRSAATLPAYAGACKQCIWYHACLHELTERNDLSL
                     IPELGRSKRDAMASRIATIRDLAAIDPANFITGKKTVFNRIGPETLEKLHARALLLTT
                     DGRPYLREPVALPVADTELFFDIEVDPMRDLCYLHGFVERRNGRNGTERFVAFFAEDT
                     TPAAEEHAFAEAWHFMQASQPCVIYCYSKYERTRYRKLQEKYPDVCTAEDIETLFAPP
                     QTVDLYFDVVKKATEWPTRDYSIKTLAGYLGFNWRDAHPSGAASIEWFDRWVRTRAPA
                     DRQRILDYNEDDCRATRVLLDGIRGLTLR"
     gene            4281..4745
                     /locus_tag="PKH10_01555"
     CDS             4281..4745
                     /locus_tag="PKH10_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26840.1"
                     /translation="MPKFITAEVENACRKCRDYSFLIPHATDVTKYKGFYQHSLDIGG
                     KIEFALPDTSDFDAKLARWDMDGENDWFRLFGMVYIHAGKITLSHLPNNAVQVDISFS
                     LDAMKAKKKGWELHLDEIKKSKMSPANKESIVKRLETRIRDFQKLIDEKAAR"
     gene            4726..5370
                     /locus_tag="PKH10_01560"
     CDS             4726..5370
                     /locus_tag="PKH10_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26841.1"
                     /translation="MKKRLVNRKRVNTMTSTPEKEPAWVASALQGITHWIGHRRCLYR
                     HYPLSEGALVAEVCNLIHANLSDEYLLKCEVQYSTLIPRDEAPAELTDRARADLVVFE
                     RMKNATNDSIARFIIEIKRASAPKAQIEADLRRLSAVRYLCRDIRTFCFIISEAQRPP
                     RFVDKKGMSIRGKMKISGSDGYFRVRRTWKAAHAYTKRDKAQYACLIEVYPSPY"
     gene            5502..6158
                     /locus_tag="PKH10_01565"
     CDS             5502..6158
                     /locus_tag="PKH10_01565"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNT26842.1"
                     /translation="MLYYDQRIVPVHGDANATVFLINEAPGPCEAYEGIPLVGTQGGN
                     LYRALRKAGIGWANQFDNFAWPSKTMKDYKSPDKMQNRFELREKFLALRAKYIACSNA
                     FPRWPRPDKDSNDWVAPECKEVLRKSNIARLRKEILPNHRVILVCGAHAWLACFGESL
                     SRPARKERRRFNKEELDKINKRFSSKFSDGWYMGHTRRWSLNADSTPRVLKKVARIAW
                     "
     gene            6248..6868
                     /locus_tag="PKH10_01570"
     CDS             6248..6868
                     /locus_tag="PKH10_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26843.1"
                     /translation="MKIPTNNLYMRLFEQYIDRNEGFTYKDVMGVPFGSPYNPSDKNN
                     IISTQAEFHSLHLLFIFADAIARKNKNIHSIVDSSFMENNPIMAFHVEDPANGPRLPV
                     EKSLFTISYGGRMAYLDYLELKHSLEFAKEAQVQSQTALHWAKIALWITAFFGVLQII
                     TGWCQIRNDNKLAATSSPMPSTYTYYECPACQQSETWPWVECKTKE"
     gene            7063..7263
                     /locus_tag="PKH10_01575"
     CDS             7063..7263
                     /locus_tag="PKH10_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26844.1"
                     /translation="MYEQIAQVAEQERTEAKALEKLLRNRAKNRLSWTEERAKRKELR
                     ILKEDIAVLDTALRIYRRMRRA"
     gene            7349..7705
                     /locus_tag="PKH10_01580"
     CDS             7349..7705
                     /locus_tag="PKH10_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26845.1"
                     /translation="MQGYLVYKRRGERTYCYLQQRHGDTVLSHYIGRKDDPQVTEIER
                     AIETRKTNEKWNKSLSQRVAILRKIIRTVIARIEKNISRRKDRTMSEYEAYLLTQWGK
                     HSTPPNNSSKKEKRKP"
     gene            7822..8532
                     /locus_tag="PKH10_01585"
     CDS             7822..8532
                     /locus_tag="PKH10_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26846.1"
                     /translation="MQFDKEKKELSLIPMKSKKGKGSPIGKKKKTGTEEARLLADLRS
                     GKVGFEDDSAEEAALKESFEKGEFKPISAARKKQLMKDAAKTLATAKKTANRFLMPYK
                     GYLGRYRYDHEARTFHGEVVGITDVVTFQAEKEKNIEKAFRDSIDDYLNACKRFGKKP
                     CKMSKKTWLQNLTPASPDFMSKRCQPENQKRPDLDIDEETTDTKRNRQIKKYGKHLKK
                     NKKSLNGFNKISEENWSE"
     gene            8620..9693
                     /locus_tag="PKH10_01590"
     CDS             8620..9693
                     /locus_tag="PKH10_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26847.1"
                     /translation="MKNKKFLEAEVLSCFQEGAILLPPLVTTTIQRQVPLPSRYVADA
                     LMDAVLPQDPETKFRFVVEAKSQSTPMIVQRALLQVKEVAGNFGLPLILVPYLASERL
                     QELEQAGVSGVDLCGNGIVIVPGRIYILRTGEPNKYKESRPLNNPYRGRSAMVARMLL
                     MRPRWNTLKELQKAIENAGTRMVISQVSKAVQALEEERMIVRSSGAVTLADAVLLLDK
                     LSRERKKPAFRSRQAVRLPLGFDSISILSTDPLLRWAVTGESSVSRYATFGQEGPRRI
                     AVSDMTVALKLLGGKPEPVLNFADVELLETDEPGYYFGNDMDNGIRWAGKVQTWLELS
                     AGDARQQEAAADIRKQILSEVKI"
     gene            9690..10574
                     /locus_tag="PKH10_01595"
     CDS             9690..10574
                     /locus_tag="PKH10_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26848.1"
                     /translation="MNGTIRDELWPYFEPLWRDLRSIDDKFLLIGGYALFLKHRWLIS
                     RPEVRTVIPLARWRDAAPRVTKDFDLIANIELIASTEDQSAVQAVLDRQGFAVVPDHA
                     RWQFEKSIGEGRDVVLEFNVVPPLAPRTDVRHDDRRVKPKPSLGDTGIHGHANPEATG
                     CDIRPFSFVIDGVEVFIPNAASIAMMKMTAMRDRWQRAQKMNAGTARDFEQAQAEKHA
                     RDVYKAVAMVTREENDTMREVLDIIRALPVFSGAADIREEFFSDPNGTGVRMITDNWE
                     QNDALGIGRMLADWFSKK"
     gene            10938..>11052
                     /locus_tag="PKH10_01600"
     CDS             10938..>11052
                     /locus_tag="PKH10_01600"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNT26849.1"
                     /translation="MIITVGNTKGGTGKSTIATTLAALYAGKKKKTLLIDAD"
BASE COUNT         3369 a         2432 c         2719 g         2532 t
ORIGIN      
        1 ccttcacctt cagacagtca ccttaactgc ctaataagca taccgataat caccttttcg
       61 gagaaatcaa cccggctctc cacatatttc cggggaacgg agatttttcc cttcctttga
      121 gtgatctcct gcaaaaaaaa gaatcctttt ggtggcacga ggtatcatat agggagaaga
      181 gagaggagcc ccatgtcctg cggtcaggcc gaacagaata attttgattt ctgtcaggga
      241 gagtcctaca atgctgactc gggagccgag aggagggaat cgggaatggg ccatcagtat
      301 ctcaaaattt tgtccgagaa agcgacaggc aaggaaggga cagacgcaat aacttaaggg
      361 aggaggacca gtatatgaat gatttaacga aaatggcgaa aaatataaat tatgacagca
      421 gaagaagttt tctgttgtct atgaaagaga ccaaactaca tgaatatatc aaagaacttt
      481 ttgtttcgat ggagccttca tattatgtag aaataactca tggctccaat gagatgggga
      541 aagacctggt tttgataagg agcgacaaac tctcaacgga agccatcgct gtcgttgtaa
      601 agaaaggtac catatcgggt aaaacagcag gggatataga tgaaataagg aatgatgtac
      661 aaaaaatatt ttccagtgga gagaggaaga tctatgatga gatcataagc cagataaggc
      721 agtccatgtg taacaaggct gaattgaaga cgctgattga taaggttata atatctaggg
      781 tgtttgtgtt tctatttggt gaacttagca atcaagcaaa agatcgtctt gatggcgaat
      841 tgaaggccta tgagggtgct gtagaatatt atcctatgaa gcgaataata gacctgttca
      901 cagattttta tccacagatt ttctttaagg gcaaattaca cgattacatt atggataaga
      961 taaaatgtct agaagacagc gtcgttatca aaaccgggaa gtctaaactt tccgaaatat
     1021 ttgttccgcc tcttatcact aaaactggtt ttccagtatt taatagtctg gaagatgcgg
     1081 cagattatac aaaaaaacag gtgttgaaaa caggcaaaga aaagatatca ctacaaaaca
     1141 ttgataaatt gttttccaac aacagaaaag taattctcat aggtgagcct ggttcgggga
     1201 aatcgtgtgc attaaataag attgcgctag atgcttttat ctctgcgcaa aaggatattg
     1261 ttgatggcaa atcgggcagc gtttctttgc caataaaact gcattcaaag aggcttgtca
     1321 atgacaatat tatagataac atcgtagagg actacatagg aaaggaggcg ctaggcgaca
     1381 acaaaataac aattaagtac attctagtag attctttaga tgaagttgac caagaagaca
     1441 gagaaaaggt aatagaaaat gcatcaaaga tatcagacaa atataatgcc ccattagtta
     1501 ttacaagtag aaagctgggg atgctggatc aacccccggc tgattttgaa aaatatgaaa
     1561 tacttccctt ttcttatgtc caagtcttga agtttatgaa aaatatgttt ttcgatgatc
     1621 ttgcaaaaat gaatataata aagaagcaaa taaatcaact aaacatgcca tactatccgt
     1681 tggctctgac tttattagtt gaattggtca gcgaatataa tgaaattcct gcatccataa
     1741 cagaattata ttcccgatat tttgatcttg ctcttggcaa ggaggacaga gacaagggca
     1801 tcagtatact gttcgaatat acaataaaaa gacagttttt agctaaactt gcatattgcg
     1861 aaatgatgtt gaaaaatcaa acatcaataa cgaaagagga atttgttgtg ttctgtgaga
     1921 attataaaag cgacattcca agtaatcact caaaagacat ggtagctttt gtaaatgaga
     1981 ttgaaagaac gggtataata aaatgtaagg ataaagtatt ttttggacat aggtctttca
     2041 tggaatactt tagtgcctac catgtgtttg aaaatcaaac agaatatgaa gatattaacc
     2101 agcatctggt ggatttgtat tttaatgaca agaatgaaga catcgtattt ttcttttgtg
     2161 gaatgaaaag aaagctgtct cagaaattag tgaaaaagat atttgaagaa aagccaaaag
     2221 atatggacga aatacaaagt atgctaataa aatacatgac accccgatta ttgcaatcgg
     2281 catttagtac aaatgaacaa acaaagatcg aaacaataat taatgctgct aatatatccg
     2341 gaaagataag gaacggtatt atgagtacat ttatgccaat aaagaacaaa ataagcccta
     2401 tattcgctga tataataatg atgaactatt ccatgtattc attgtcaagt atgtttataa
     2461 aaaacgaaat aattgaagct ataaaggtga tatcgaaaaa cacagagcca gaagtacctt
     2521 atatgatgat gtatctcctt gggggaatag aggagaaaat atctattgaa gaaaaggatg
     2581 aaataattaa gaagataatt gacacgatga taaacaacaa gatatcgaaa aaggatgaag
     2641 cacaaatact gttattttta attaagatgg aagaaaacaa caaatcaata aagtcagcaa
     2701 cgcgaaaact cgaaaaaatt aaacgtagaa acaaggaact atttaacaca ttgttcattc
     2761 ctcaaaaaat aaagaagttg ttataaacat tgcaaacact acccccctcc cccatcacgg
     2821 cctccatgct ctacgacttg gtcggttgcc cccaccgcgt caccatggac ctcttcggga
     2881 acccggccga ccgtgatccg gtcaatgcct tcgttcaact cctctgggat cgtggcgccg
     2941 cccacgagga tgatgtcata gcaacccttg atattccccc tctcgacctc tccgggttat
     3001 ccagtgcgga aaaggaagcg cgcacgatcg aggcaatgaa ccgtggcgtc cctctcatct
     3061 acagcggccg catcaccgtg gacaacctca ttggcgaacc ggaccttctg cggaaagagg
     3121 gccccggcta cattgccggc gatatcaaat cgggcgccgg agaagaaggc ggcgatgatg
     3181 gggatgacgg taagtccaag aagcactacg cagtccagct tgccctctac accgacatct
     3241 tggagcgtct cggccggtcg gctggtcgtc gggccttcgt atgggatatt gctcgaaacg
     3301 aggtccccta tgacctcacg ggttctctct gggatcttta tcagagctgc ctctcacaag
     3361 cacagtccat aaccgaccgg agcgccgcta cgctcccggc ctatgccggc gcctgcaaac
     3421 agtgcatctg gtaccacgcc tgcctgcatg aactgacgga aaggaatgac ctctccttga
     3481 taccggaact gggccgatca aaacgggacg cgatggcctc ccggatagcc accatccgcg
     3541 atcttgcggc gatagatcca gccaacttca tcactgggaa gaaaacggta ttcaaccgca
     3601 tcggccccga gaccctcgaa aagctacatg cccgcgcgct tctcttgacc acggacggac
     3661 gcccctacct tcgggagccc gtcgcactgc cggtggccga cacagagttg ttttttgaca
     3721 tcgaggtcga tcccatgcgt gacctctgct acctgcatgg gttcgttgaa cgccggaacg
     3781 gccggaacgg caccgagcgt ttcgtcgcgt tctttgccga agatacgacc cccgcagcgg
     3841 aggaacacgc cttcgccgag gcatggcact tcatgcaggc atcgcagccc tgcgtcatct
     3901 actgttactc gaaatacgag cgcacgagat accggaagct tcaagagaag tatcccgatg
     3961 tttgcaccgc agaggatatc gagacgctgt tcgctccgcc acaaacagtc gatctctatt
     4021 ttgatgtggt gaagaaggca accgaatggc cgacacggga ctactcgatc aagaccctcg
     4081 cgggctatct tgggttcaac tggcgggacg cgcacccctc cggggcggcg tcgatcgaat
     4141 ggttcgaccg ttgggtccgg acccgagccc cggccgacag gcagaggatc cttgactaca
     4201 acgaagacga ctgccgggcg acgagggttt tgctggatgg gattagggga ctgactttga
     4261 ggtaggaggg agaaggtatt atgccgaaat tcattactgc tgaagtagaa aacgcttgcc
     4321 gtaaatgtag ggattattct tttcttatac cgcatgctac tgatgtcacg aagtacaaag
     4381 gattttacca acacagctta gacattggcg ggaaaataga gttcgcgttg cctgatacct
     4441 cggattttga tgccaagttg gctcgttggg acatggacgg cgaaaacgat tggttccgct
     4501 tgtttggcat ggtttatatt catgctggca agataaccct tagccattta cccaacaatg
     4561 ccgtccaagt tgatatatct ttctcgcttg acgcaatgaa ggcaaaaaag aaagggtggg
     4621 aattacattt agatgaaatc aagaaaagca agatgtctcc tgctaataaa gagagcattg
     4681 tcaagcgtct tgaaacacga attcgtgatt tccagaagct aatagatgaa aaagcggctc
     4741 gttaatcgca agagagttaa taccatgacc agcactcccg aaaaagagcc cgcgtgggtt
     4801 gcaagcgcac tccaaggtat tacccactgg ataggtcata ggcgttgcct ttatcggcat
     4861 tacccgctat cagaaggcgc attagtggcc gaggtgtgca atctcattca cgccaaccta
     4921 tctgatgagt atttgctcaa atgtgaagtg cagtattcga cgctcatacc tcgagatgaa
     4981 gcccccgcgg aacttactga tcgagcaaga gccgaccttg tcgtttttga aagaatgaaa
     5041 aatgctacca atgattcgat tgcaagattt ataattgaga tcaagcgggc ctcggcgccg
     5101 aaagcccaaa ttgaggcgga tcttcgtcgc ctttctgctg ttcgatatct ctgccgtgat
     5161 attcgaacat tttgtttcat catatctgaa gcgcaacggc caccaaggtt cgttgacaaa
     5221 aaaggcatgt ccattcgggg caaaatgaag atttcagggt cagatggata tttcagagtt
     5281 aggcgcactt ggaaagctgc tcacgcatat acaaaaagag acaaagcgca gtatgcgtgt
     5341 cttatagaag tatatccatc gccgtattga cctcgaactc ctatccatga gacggtattt
     5401 cgtctcaatg ggaaagcgtc cctccgccta taaaaattga tttcctcacg aggaggtcta
     5461 aaattcttga gatgaaaggg aaagcaaggc ggataaagga aatgctctac tatgatcaga
     5521 ggattgttcc tgtacatggg gacgcaaacg ccacagtatt tctgatcaac gaagctcccg
     5581 ggccatgtga ggcatatgaa ggtattcctt tggtcgggac gcaaggaggc aacctttata
     5641 gggcgcttcg gaaagccgga ataggatggg ccaaccaatt cgataacttc gcatggccca
     5701 gcaagactat gaaggattat aaaagccccg acaagatgca aaatagattc gaactcagag
     5761 aaaagttcct tgccctccga gcgaaatata ttgcttgctc aaacgctttc cctcgatggc
     5821 cacggcctga caaagattcc aatgattggg tagccccaga atgtaaagag gtactgagaa
     5881 aatctaatat cgccagacta agaaaagaaa tattgccgaa tcatcgagtg atcctcgtgt
     5941 gcggagcaca tgcatggctg gcatgtttcg gagagtctct ttctcgccct gccagaaaag
     6001 aaagacggcg ctttaataaa gaagaactgg acaagataaa taaaagattc tcatcgaaat
     6061 tctctgatgg ttggtacatg gggcatacgc gcagatggtc tcttaatgct gacagcacac
     6121 cccgtgtcct gaagaaagtg gcgcgcattg catggtaatg tcaatcgtga tcgccaaggg
     6181 cgactacgac gcggggagag agtggttgga tgggtttttt aggaagtagt agagaaagga
     6241 ggtcaagatg aagattccca ccaacaatct ctatatgagg ttgttcgaac agtatattga
     6301 tcgcaacgaa gggttcactt ataaagatgt aatgggcgtt ccctttggtt cgccatacaa
     6361 cccgagcgat aaaaataata taatttctac ccaagcagag tttcattcgt tacatcttct
     6421 ttttatattt gccgatgcta tcgcaagaaa aaacaaaaat attcattcaa tagttgattc
     6481 ttcttttatg gaaaacaatc ccataatggc gtttcatgtt gaagatcctg ctaacgggcc
     6541 aagattacca gttgagaaat cattgtttac tataagctat ggtggccgaa tggcctatct
     6601 tgactatctc gaacttaaac actctttgga gtttgcgaaa gaggctcaag tccaatctca
     6661 aacagccttg cattgggcca agatcgcatt atggataact gctttttttg gtgtacttca
     6721 gataattaca ggctggtgcc agatacggaa cgacaacaaa ctcgccgcca catcatcacc
     6781 aatgccgtca acatatacat actacgaatg tccggcctgc cagcaatcgg aaacttggcc
     6841 gtgggttgaa tgtaaaacca aagaataggg acaaaatagg tccctcccct cccctaccat
     6901 aataaatttg atttctgagc agaagaggac cttcatagat cgcatcctac cctcgggaga
     6961 ctgaccctac aaaatagata taaacgaaac gggacgagaa tcttttcttg acaaggaaat
     7021 gccctacaaa taatataaaa tccgaacaga aagcggtatg ccatgtacga acagatcgca
     7081 caggtcgcgg aacaggaacg gacggaagcg aaagcgctgg aaaaacttct gaggaatcgc
     7141 gcgaagaacc gactgtcttg gaccgaagag cgagccaaga gaaaagaact tcgcatattg
     7201 aaggaagata tcgctgttct cgatacggcc ctccgcatct accggagaat gcgccgtgct
     7261 tagatattca tctctcactt ggatcatagt tcaacatgac caatgggtcg aatcgataga
     7321 atggggagaa aggcacattc gcaatgccgt gcagggctac ctcgtgtata aacgacgggg
     7381 agaaagaact tattgttacc tgcaacagcg gcacggggac accgttcttt cacactatat
     7441 cggtcggaaa gacgatccgc aagtgacgga gatcgaacgc gctatagaaa ctcgcaagac
     7501 caacgagaaa tggaataagt cgctttccca aagggtcgcg atactccgca agatcatcag
     7561 gaccgtcatc gcccggatag agaaaaacat atcacgccgc aaggaccgca cgatgagtga
     7621 atacgaagca tacctcctga cacaatgggg caaacactca accccgccca acaactcttc
     7681 gaaaaaggag aaaagaaagc catgaaaaag cagagggagc gtaccgtgga agtgaaagcc
     7741 gagatagtca aaaagggcaa agaatattat cttattcttc cgtcggagat cctcggcaaa
     7801 ctgaactgcg acacattcga catgcagttc gacaaagaga aaaaggaact gagccttatc
     7861 ccgatgaaaa gcaaaaaggg caagggttcc cccatcggta agaagaaaaa gaccgggacg
     7921 gaagaggccc gcctgctcgc cgacctccgt tcggggaaag tcgggttcga ggacgattcc
     7981 gccgaagaag ccgcgctcaa ggaatctttt gaaaaaggag aattcaagcc gatctctgcc
     8041 gcgcgtaaaa aacaattaat gaaggacgcc gcgaagacct tggctacagc aaaaaaaacg
     8101 gcaaatcgct ttttgatgcc atataagggg tatctggggc ggtaccgata tgaccatgag
     8161 gcccggacct ttcatggtga agtggtcggc ataacggatg tcgtcacctt tcaggccgag
     8221 aaagaaaaaa acattgaaaa agcgttccgt gactccattg acgattatct caacgcctgt
     8281 aagcgttttg gcaaaaagcc ctgtaagatg agcaaaaaga cttggcttca gaatttgacc
     8341 cccgcttctc cagacttcat gtcgaaacga tgtcagccgg agaaccaaaa gagacccgat
     8401 ctcgacatcg atgaggaaac gacggacaca aaacgcaacc gacagattaa aaagtacggg
     8461 aaacatctta agaaaaacaa aaagtccttg aatggattca acaaaatctc cgaagagaac
     8521 tggtccgaat aacactattt cctaaacggg aaaacttgac aaaacaagaa aacaaactat
     8581 gttttccttg aggatggtta tttcctaaac gggaaaacga tgaaaaacaa gaaattttta
     8641 gaggccgagg tgctctcgtg ctttcaggaa ggggcgatat tgttgcctcc tttggtcacc
     8701 accacaatac agaggcaggt tcccctaccc tcccgctatg ttgccgacgc actcatggat
     8761 gccgttcttc cacaggaccc cgagacgaag ttccgtttcg ttgtggaggc aaagtcacag
     8821 agtaccccga tgatcgtaca gcgcgctttg ttgcaggtga aagaggtggc gggcaatttc
     8881 gggttgcctt tgatactggt accatatctt gcttcggagc ggttgcaaga actggagcag
     8941 gcgggggtca gcggtgtcga tctgtgcggg aacggtatcg tcatcgtacc gggacggata
     9001 tatattcttc gtacggggga acccaacaag tacaaggaat cacgcccact caacaatccg
     9061 tatcgcggtc ggtcggcgat ggttgcgcga atgttgctca tgcgcccccg ttggaatacg
     9121 ctcaaagaac tgcaaaaagc gatcgagaac gccgggaccc gcatggtcat ctcgcaggtt
     9181 tccaaggcgg tgcaggcgtt ggaagaggag cggatgatcg tcagatcgag cggcgctgtt
     9241 acgcttgccg atgccgttct tttgttggat aagttgagcc gtgagagaaa aaagccagcg
     9301 tttcgttcgc gacaggcagt acgattaccg ttgggttttg acagcatctc cattctttcg
     9361 accgatcctt tattgcggtg ggcggtcacc ggagaatcat cggtatcgcg gtatgcgacc
     9421 ttcggacagg aaggaccgcg caggatcgcc gtttcggata tgaccgtcgc tttgaaactg
     9481 ctcggcggga aacctgagcc ggtgctcaac ttcgccgatg tggaactgtt ggaaacggac
     9541 gagccggggt attatttcgg gaacgacatg gataacggta tccgctgggc cggcaaggtc
     9601 caaacctggc tcgaactgtc ggcgggcgat gcgcgccaac aggaagcggc ggcggatatt
     9661 cgcaaacaga tactcagcga ggtaaagata tgaacggaac catacgggat gaattatggc
     9721 catacttcga accgctgtgg agagatctac gatcgatcga tgataaattc cttctgatcg
     9781 gcggatatgc tttgttcctg aaacatcgat ggcttatttc ccgaccggaa gtgcgcaccg
     9841 tcattccgct ggcgcgatgg cgggatgccg ccccgcgcgt taccaaagat ttcgatctca
     9901 ttgcaaatat tgaactgatc gcttcaaccg aggaccaatc ggcggtacaa gcggttctcg
     9961 accgtcaggg ctttgccgtg gtgcccgacc atgcgcggtg gcaatttgaa aaaagcatag
    10021 gtgaaggccg ggatgtcgtt ctggagttca atgtggtgcc gccactagcg ccgagaaccg
    10081 atgtcagaca cgatgatcgc agggtaaaac cgaagccttc tctgggcgac accggcattc
    10141 atggtcatgc caatcccgag gcgactggtt gcgacatacg ccctttttct ttcgtaattg
    10201 acggtgtcga ggtgtttata ccgaacgcgg caagtatcgc gatgatgaaa atgaccgcga
    10261 tgcgcgaccg ctggcaacgg gcacaaaaga tgaatgccgg caccgccaga gatttcgagc
    10321 aggcgcaggc cgaaaaacat gcgcgggatg tgtacaaggc ggtggcgatg gtcaccagag
    10381 aggaaaacga cacgatgcgc gaagtgctgg atatcatacg ggcgctaccg gtgttttccg
    10441 gggcggcgga catccgggaa gaattctttt cggatccgaa cggcaccggg gtgcgcatga
    10501 taaccgataa ttgggaacag aacgacgccc tagggatcgg aaggatgctt gccgactggt
    10561 tttcgaaaaa ataaagcgta agatccatca tctcctttac agagacaatc aagaaaaaag
    10621 aagaaaacaa ataagtaaat atgtacatat gtatttacgg caagagagca tttccggaaa
    10681 gacagcgatg atcgaaccgg aaaggatcgt gtggagaggc gcggcaacaa aacgaaaaag
    10741 catatgtaaa taaatatgta tttatgtgtg tgctggcttg caggcgaaac atctacgcgc
    10801 tatgaaatat gtacgtacat aagtatgtgc gataaaaacg agaaaacaaa cttgacatat
    10861 acttgcatat gtatttaatg atgtacatga gtatgtgcgc acatacatgc ttacataagt
    10921 acaagggagg ttgatcgatg atcattaccg tagggaacac caaaggaggg acgggcaaga
    10981 gcaccattgc taccacgctc gccgctctct atgccgggaa aaagaaaaag actctgctca
    11041 tcgacgccga tg
//
LOCUS       DAOQUE010000027        10981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_6702_length_10981_cov_4.363262, whole genome shotgun sequence.
ACCESSION   DAOQUE010000027 DAOQUE010000000
VERSION     DAOQUE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6702_length_10981_cov_4.363262"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PKH10_01605"
     CDS             complement(<1..552)
                     /locus_tag="PKH10_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HNT26850.1"
                     /translation="MMQDGRYLYLFLLGKPALGKGTQIRRVLETFEGTSFSTGDALKD
                     HIARGTELGKRVKSLIESGALVSDDIVMALFKDSAQRLVPVKGRALFLVDGFPRTIGQ
                     YEMVRTYLCEQDASYSFVYFSIDDEVILRRFAGRLVCDACFSPFSTLSSSLKEKDTCP
                     LCKKGALIRRADDNEAVVRNRLAE"
     gene            complement(559..1875)
                     /gene="secY"
                     /locus_tag="PKH10_01610"
     CDS             complement(559..1875)
                     /gene="secY"
                     /locus_tag="PKH10_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNT26851.1"
                     /translation="MSRILSNIFKIPDLRERVLFSLFILAVYRMGIFVTTPGVDKNIM
                     ADIMATKGSTTILGLFNMFSGGALEQLSIFALGIMPYISSSIIFSLLAVVIPQIGEMQ
                     KEGEQGQKKIVQYTRYGTVLLCIFQGLMMAKFLESLTGRAGEVVVQDPGWFFRALTVI
                     TLTTGTLFLMWLGEQISERGIGNGISLLIFASIVARFPNMFSQLIKYLNEEMIKPFHL
                     VIFILVLVLAFTVIVFVESSRRKIPIQYARRVSGGRTLQNSYLPLKINTSGVIPPIFA
                     SALLVFPATIGGFLNAEWMGTLQTMLNPGGWLYELLYIFLIIFFAFFYTFVQFKTDDV
                     AENLNKNGGYIPGIRPGKETVAYINKVLSRITFGGAIYLTAICLIPDLLRDKMGLPFY
                     MGGTSILIVVGVAMDTASQIESYLISNNYEGFSIGGKGSLVKSRSR"
     gene            complement(1868..2317)
                     /gene="rplO"
                     /locus_tag="PKH10_01615"
     CDS             complement(1868..2317)
                     /gene="rplO"
                     /locus_tag="PKH10_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007961558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNT26852.1"
                     /translation="MRLHELKKPEGLKSRKIIGRGNGSGHGRTSTKGHKGQRSRAGRS
                     VRRGFEGGQMPLIRRLPKRGFFNLFRVEVTEINVRYLDKFEAGTTVDPVKLAEAGLCK
                     GNETCIKIIGRTELQHPLTVIAHKFTKGAKESIEKVGGKAEEMKLDQ"
     gene            complement(2327..2845)
                     /gene="rpsE"
                     /locus_tag="PKH10_01620"
     CDS             complement(2327..2845)
                     /gene="rpsE"
                     /locus_tag="PKH10_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNT26853.1"
                     /translation="MKEKQKPINPEGLDLREKLIYVNRVAKVVKGGKNFSFAALMVIG
                     DGHGHVGLGLGKAREVPEAIKKGIARARKNLRHITIEKGTIPHEVLGEFGAGRVFLRP
                     ASPGTGVIAGSAVRSVLELVGVTDILTKSIGSSNPHNMAKATLKALLSMENMDEVQEK
                     RNISIVDYQSKK"
     gene            complement(2872..3234)
                     /gene="rplR"
                     /locus_tag="PKH10_01625"
     CDS             complement(2872..3234)
                     /gene="rplR"
                     /locus_tag="PKH10_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNT26854.1"
                     /translation="MDRKKKIEQRIRRKTSIRKRIGEGSPSRPRLTVFRSSQNIYAQV
                     VDDISGTTLVSASTVGKDYKEGEGDKKADAARVGELIAMKCKAKGITTVVFDRNGYLY
                     HGRVKALAEAARKGGLKF"
     gene            complement(3261..3797)
                     /gene="rplF"
                     /locus_tag="PKH10_01630"
     CDS             complement(3261..3797)
                     /gene="rplF"
                     /locus_tag="PKH10_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNT26855.1"
                     /translation="MSRIGKKPIPFDSKVEVSLKENMLRVKGPKGTLEQAIHPKITVR
                     IEKNVITVERADDEKATKALHGLFRALIHNMVTGVTTGFSRVLEVKGTGYKFEMKGNK
                     LSFLLGFSHPVEFDIPKGITAEVKPGELTLTAADKQLLGDFSSKIKALRPVEPYKAKG
                     ISYRGQVIRRKAGKAAGK"
     gene            complement(3813..4205)
                     /gene="rpsH"
                     /locus_tag="PKH10_01635"
     CDS             complement(3813..4205)
                     /gene="rpsH"
                     /locus_tag="PKH10_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007230697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNT26856.1"
                     /translation="MNDLISNYLTRLNNALRAKHETVVGIPSSRVVEGVSKVLKEEGF
                     ISDFRVTDVAGKKTIAVDFRYHKGKPVISHIERVSKPGRRVYVPVKEIPYVRAGLGVC
                     VVSTSKGVLTDKKARAINVGGEILCKVW"
     gene            complement(4219..4404)
                     /locus_tag="PKH10_01640"
     CDS             complement(4219..4404)
                     /locus_tag="PKH10_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNT26857.1"
                     /translation="MARLTMMLKAKRKPAFSTRYHNRCPLCGRARSYIRKFDMCRLCF
                     RKMASNGLIPGVIKSSW"
     gene            complement(4430..4969)
                     /gene="rplE"
                     /locus_tag="PKH10_01645"
     CDS             complement(4430..4969)
                     /gene="rplE"
                     /locus_tag="PKH10_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNT26858.1"
                     /translation="MSRMRTYYQTEIRPALQKELGITNPMAVPRIERIVLNMGVGEAT
                     QNKKLLEDAAKELALIAGQRPVINKARKSIATFKLREGTPIGTSVSLRKDRMYDFLDR
                     LISVALPRVKDFRGVSGKAFDGRGNYSLGIKDFMIFPEIDFNKVENVKGLNVTICTSA
                     RDDKAAKALLKMFGMPFNN"
     gene            complement(5109..5429)
                     /gene="rplX"
                     /locus_tag="PKH10_01650"
     CDS             complement(5109..5429)
                     /gene="rplX"
                     /locus_tag="PKH10_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNT26859.1"
                     /translation="MSTMANIKKNDTVRVITGKDKGKTGKVLRVITDTEKVVIENVNV
                     YKKHQKQGQNREAGIVDKAMPIHISNVRAICPKCAKATSFRRKVLSDGKRVRFCRACG
                     ETIE"
     gene            complement(5440..5808)
                     /gene="rplN"
                     /locus_tag="PKH10_01655"
     CDS             complement(5440..5808)
                     /gene="rplN"
                     /locus_tag="PKH10_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNT26860.1"
                     /translation="MIQEQTRLAVADNSGAKEVQCIKVYGGSKRRYAGIGDVIVVSVK
                     DAEPKGKVKKGEVKRAVIVRQAKELKREDGSYIRFDDNAVVILDDYYEPLGTRVFGPV
                     ARELRIKGFMKIISLAPEVL"
     gene            complement(5805..6071)
                     /gene="rpsQ"
                     /locus_tag="PKH10_01660"
     CDS             complement(5805..6071)
                     /gene="rpsQ"
                     /locus_tag="PKH10_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNT26861.1"
                     /translation="MKAGSHKRTLVGVVVSDKAEKTVTVSVQRRYKHPLYKKMITTRK
                     KYLVHDEQNLCKIGDRVRISECAPLSKRKRWLIVERIALQGDGA"
     gene            complement(6077..6286)
                     /gene="rpmC"
                     /locus_tag="PKH10_01665"
     CDS             complement(6077..6286)
                     /gene="rpmC"
                     /locus_tag="PKH10_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNT26862.1"
                     /translation="MKKVEMKKKSREELKDLLLVLEEEHFNLRVQKAMGQLQNTARIR
                     LVKKDIARVHTFLAAPSQNTVTDKR"
     gene            complement(6283..6702)
                     /gene="rplP"
                     /locus_tag="PKH10_01670"
     CDS             complement(6283..6702)
                     /gene="rplP"
                     /locus_tag="PKH10_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNT26863.1"
                     /translation="MLMPNKVKYRKMMKGRMAGISTACNAIDFGEYGLKALECGWVTS
                     RQIEAARVAIMRIARKGGRMWIRVFPDKPITKKPLETRMGKGKGNPEEWVCVVKPGRI
                     MFELGDLDAASAKEALRLASYKLPIKTKIVMKGDEIR"
     gene            complement(6708..7397)
                     /gene="rpsC"
                     /locus_tag="PKH10_01675"
     CDS             complement(6708..7397)
                     /gene="rpsC"
                     /locus_tag="PKH10_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNT26864.1"
                     /translation="MGQKVNPISLRVGITRTWSSVWYSHRNYAKWFHEDLKIREAIKK
                     EFYLSGIAAVTIERSGEKIKVVIRTAKPGLVVGKQGAGIERIKQIVGKIAQNAQEVFV
                     QPEEIKKPEINAQLVSESIAEQIEKRAHFRRVMKRAMGNAIKSGIEGIKVQVGGRLGG
                     NEIARVEHYHEGRTPLHTLRADIDFGHAVARTTYGTIGVKVWIYKSEVSKRYDSRRKP
                     AEKRSTSSQDR"
     gene            complement(7422..7778)
                     /gene="rplV"
                     /locus_tag="PKH10_01680"
     CDS             complement(7422..7778)
                     /gene="rplV"
                     /locus_tag="PKH10_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006691174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNT26865.1"
                     /translation="MEDKTVKATLKYYRMSPRRVREVADVIRGKRVEDAQNILDFTNR
                     RAAGALKKLLSSAIANAGNNFGLDPDRLFVVAIAVDEGPMWKRFITKCRGQASRIVKR
                     TSHITIELGEKKEKKG"
     gene            complement(7845..8114)
                     /gene="rpsS"
                     /locus_tag="PKH10_01685"
     CDS             complement(7845..8114)
                     /gene="rpsS"
                     /locus_tag="PKH10_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNT26866.1"
                     /translation="MPRSVKKGPFVDAHLWKKVVKAIDSSDRKPIKTWSRRSTIVPEM
                     VGLTFAVHNGRKFLPVFITEEMVGFKLGEFSPTRTFKSHAGEKKK"
     gene            complement(8150..8980)
                     /gene="rplB"
                     /locus_tag="PKH10_01690"
     CDS             complement(8150..8980)
                     /gene="rplB"
                     /locus_tag="PKH10_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNT26867.1"
                     /translation="MGIKTYNPTSAGRRNMSVADHTVLTEKRPEKALVRSLKKSGGRN
                     AYGRITSRRRGGGNKRLYRIIDFKRDKINVPGKVAAIEYDPNRNAHIALIHYLDGEKR
                     YIIAPEKLEVGDTIVSADKDVDVRPGNAMKLRYIPVGTVIHNVETKQGKDSQIGRSAA
                     SGIQLLGKEGDYATVRMPSGEIRIIHLECRATIGQVGNSEASNVRIGKAGKSRWLGRR
                     PKVRGVVMNPVDHPHGGGEGKTSGGRHPVSPWGKPTKGYKTRNNKRTDRFRVRRRGKK
                     "
     gene            complement(8999..9289)
                     /gene="rplW"
                     /locus_tag="PKH10_01695"
     CDS             complement(8999..9289)
                     /gene="rplW"
                     /locus_tag="PKH10_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011180912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNT26868.1"
                     /translation="MRYFGIIKRPIITEKSTALSRGEEKGYLFEVSMDAEKDDIKAAV
                     ESLFDVKVKEVNTVIVRGKVKQVKRVTGKRNNRKKAYVTLQKGYSINFIEGV"
     gene            complement(9295..9927)
                     /gene="rplD"
                     /locus_tag="PKH10_01700"
     CDS             complement(9295..9927)
                     /gene="rplD"
                     /locus_tag="PKH10_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNT26869.1"
                     /translation="MPVIDVFDRDKKKVGDITLHDEVFAVNWQERQVPVYEVVRMQTA
                     GWRSGTHATKTVSTVSGGNSKPFRQKGTGRARRGTMRAGIMRGGYVILGPQPRDYSYT
                     VPAKKRRLAMKAVLSERLEKGSLFVIKGFDFDTVKTREAKSLLKKTWDLSEAIIITGD
                     REEKFALSVRNIPEYLVLHFSQVNLYDIMAYKTILMTEEAAKYINEVYVS"
     gene            complement(9939..10565)
                     /gene="rplC"
                     /locus_tag="PKH10_01705"
     CDS             complement(9939..10565)
                     /gene="rplC"
                     /locus_tag="PKH10_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNT26870.1"
                     /translation="MSAGLIGKKVGMTQIFNEDGVSIPVTVIEAGPCFIIQKKSTEKD
                     GYCAVQVGFEQKSKNVRKPEAGHFKSAGKGIFRRLKEFRVNEKDLQGLNLGDELKADL
                     FKVGEKVKVTGFTKGRGFTGVVKRWNFSGGSDAHGSKFHRKGGAVGQNVHPGKIWKGK
                     RMAGRLGNEQVTIKNLEVVKIYPEKNLLLLKGAIPGATGGVVYIKKSK"
     gene            complement(10604..10912)
                     /gene="rpsJ"
                     /locus_tag="PKH10_01710"
     CDS             complement(10604..10912)
                     /gene="rpsJ"
                     /locus_tag="PKH10_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNT26871.1"
                     /translation="MSDRIRIKLKAFDHKLLDGAVSEIIDTVIRTGARIAGPIPLPTK
                     IHKYCVLRSPHVDKKSREQFEMRVHRRILDILNPTTQTMDELMKTDLSAGVEIEIKTS
                     "
BASE COUNT         2112 a         3295 c         2782 g         2792 t
ORIGIN      
        1 ctcggcgagc cggttgcgca ccacggcctc gttatcgtcg gcgcgacgga tcagcgcacc
       61 ttttttgcac aacgggcagg tatccttctc tttcagtgag gaactcaggg tggagaaggg
      121 ggaaaaacag gcatcacaca cgagccgtcc cgcgaaccgg cgaaggatga cctcgtcgtc
      181 tatggagaag tacacgaacg aataggacgc gtcctgctcg cacaggtagg tgcggaccat
      241 ctcgtactga ccgatggtgc ggggaaaccc atccacgagg aatagcgccc gccccttgac
      301 cgggacaagt cgctgggcac tatccttaaa gagggccatc actatgtcgt ccgacaccag
      361 cgcgccgctc tcgatgaggc tcttcacccg tttaccgagt tcggtacccc gggcgatatg
      421 gtccttcaat gcatcaccgg tggaaaaaga ggtcccctcg aaggtctcga ggacacgacg
      481 gatctgggtc cctttgccca aagccggttt accgagaaga aagagataga gatagcgccc
      541 gtcctgcatc atctgcccct agcggctcct gcttttgacg agcgagcctt taccaccgat
      601 ggagaagccc tcgtagttgt tgctgatgag ataggactct atctgggagg cggtatccat
      661 ggcaacgccc acgacgatga ggatggaggt cccacccatg tagaagggaa gccccatctt
      721 gtcgcgcagg agatcgggta tcagacatat cgccgtcagg tagatagcgc cgccgaaggt
      781 tatccggctc agaaccttgt tgatgtaggc gaccgtttcc ttgccggggc ggatgccggg
      841 tatgtacccg ccgttcttgt tgaggttctc ggcgacatcg tcggtcttga actgcacgaa
      901 tgtgtagaag aatgcgaaga agatgataag gaagatatac agcagttcat agagccagcc
      961 gcccggattg agcattgttt ggagcgtccc catccattcc gcgttcagaa atcctccgat
     1021 agtggctggg aagacgagca gagcgctcgc aaatatcggg ggaatgacac cggatgtgtt
     1081 tatcttgagc gggagatagc tgttctgcaa ggtcctgccg ccactcaccc gtcgcgcgta
     1141 ttgtatcggt atcttgcgac gggaactctc cacgaacacg ataacggtga aggcaaggac
     1201 cagcaccagg atgaaaatca ccagatggaa cggctttatc atctcctcat tgagatactt
     1261 tatgagttgc gagaacatat tggggaagcg ggccactatg gaggcgaaga taagcaacga
     1321 gatcccgttg ccgataccac gctccgagat ctgttcaccc agccacatca ggaacagggt
     1381 gccggtcgtc agggtgataa ccgtcagcgc gcggaagaac caccccgggt cctgcaccac
     1441 cacctcgccg gcacgaccgg taaggctttc aaggaacttc gccatcatga ggccctggaa
     1501 tatgcaaaga agcaccgtgc catagcgggt gtactgcacg atcttcttct gtccctgctc
     1561 gccttctttc tgcatctctc ctatctgcgg gatgaccacc gccagaaggg agaaaatgat
     1621 ggaggagctg atatagggca tgatgccgag cgcgaagatg gagagttgct cgagcgcgcc
     1681 gcccgagaac atgttgaaaa ggccgaggat cgtcgtcgag cccttcgtcg ccatgatatc
     1741 ggccatgata ttcttgtcga caccgggcgt cgtcacgaat atccccatcc ggtagaccgc
     1801 aagaatgaag agcgagaaaa ggacccgctc gcgcaggtcg gggatcttga atatgttgga
     1861 gagtatccta ctgatcaagt ttcatttcct ccgcttttcc accgactttc tctatcgatt
     1921 ccttcgcccc tttcgtgaat ttgtgggcga taacggtcag cgggtgctga agttccgtgc
     1981 ggccgatgat cttgatgcag gtctcattcc ccttgcaaag tcccgcttcc gcaagtttca
     2041 ccggatcgac ggtggtcccc gcctcgaatt tatccagata gcggacattg atctcggtca
     2101 cctcgacccg gaacaggttg aaaaagcctc tcttggggag ccgtctgata agcggcatct
     2161 ggccaccttc gaacccccgc cggaccgatc gaccggctct ggaacgctgt cccttgtgcc
     2221 ctttggtgga ggtcctgcca tgtcccgacc cattgccgcg cccgatgatc tttctcgatt
     2281 tcaggccttc cggtttcttc agttcgtgga ggcgcatggg tatctcctat ttctttgact
     2341 gataatcaac gatggaaatg tttcgcttct cttgcacttc gtccatattc tccatggaaa
     2401 gaagtgcctt cagcgtggcc ttagccatat tgtgcggatt actggacccg atgcttttgg
     2461 tgagaatgtc ggtcacgccg acaagttcaa gcaccgagcg gaccgctgag ccggcgataa
     2521 cgccggtacc gggcgaagca gggcgcagga agaccctgcc ggcaccgaat tccccgagca
     2581 cctcatgggg gatcgttcct ttctctatcg ttatatggcg caggttcttg cgtgcccgcg
     2641 cgatgccctt ctttatcgct tccggaacct cgcgcgcctt gccgagacca agcccgacat
     2701 gaccatgacc gtcaccgatc accatgagcg cggcgaaact gaaattcttg ccgcccttga
     2761 cgaccttggc gacccggttc acatagatga gtttctcgcg aagatccaac ccttcgggat
     2821 tgatcggttt ctgcttctct ttcatcactc ttgcctcgtt ccgttcgctg tttaaaactt
     2881 caggccgcct ttacgcgccg cttcggcaag agccttgacg cggccatgat acagatatcc
     2941 gttacggtcg aacacgaccg tggtgatccc tttcgctttg cacttcatcg cgatgagttc
     3001 gcccacccgc gcggcgtcgg ctttcttgtc cccttcgcct tccttgtaat ccttgcccac
     3061 ggtcgacgcc gagaccagcg tcgttcccga gatgtcgtcc acgacctgcg cgtatatgtt
     3121 ctggctgctc cggaaaacgg tcaggcgcgg acgcgagggg gaaccctccc ctatcctctt
     3181 acggatcgat gtcttccttc tgatccgttg ctctattttc ttttttctgt ccatgatgta
     3241 acctcgttcc ctctatccgt ttatttgcct gccgcctttc ccgctttgcg ccggatcacc
     3301 tgaccgcggt aactgatgcc cttggctttg tacggttcta ccgggcgcag agcctttatc
     3361 ttcgaggaga aatcccccaa caactgcttg tcggccgccg tcagcgtaag ttcgcccggc
     3421 ttgacctcgg cggtgatccc cttgggtatg tcgaactcga cgggatgcga gaatcccaac
     3481 aggaaggaaa gcttgttccc tttcatctcg aacttgtaac cggtaccttt cacttcgagc
     3541 acgcgagaga aaccggtggt cacgccagtc accatgttgt gtatcaacgc gcggaacaga
     3601 ccgtgcaaag ccttggtggc tttctcgtca tcggcgcgtt ctaccgtgat gacattcttc
     3661 tcgatgcgga ccgttatctt ggggtggatc gcctgttcga gcgtcccctt gggacccttg
     3721 acccgcaaca tgttctcttt cagcgacacc tcgaccttac tatcaaaagg tatcggtttc
     3781 ttgcctattc tcgacacggt aacctccttt cgctaccaga ccttacaaag gatctcgccg
     3841 ccgacattga tagcgcgcgc ttttttgtcg gtcaacaccc ctttcgaggt ggagaccacg
     3901 cacacgccaa gcccggcacg cacatacggt atctccttga ccggcacata cacacgacgc
     3961 cccggtttgc tgacacgctc gatgtgggaa atgaccggct tgcctttgtg gtaacggaaa
     4021 tccacggcta tggtcttttt cccggccaca tcggtgacgc ggaaatcgct gatgaagccc
     4081 tcttctttga gcaccttgct gaccccttca accacgcggg atgaagggat gccgacgacc
     4141 gtttcgtgtt tggcccgaag cgcgttattg agccgcgtca agtagtttga tatcagatcg
     4201 ttcatccttc tctcctgttc accagcttga ttttatgaca ccggggataa ggccgttgct
     4261 cgccattttg cgaaaacaaa gtcggcacat atcgaacttc cttatgtagg agcgagcacg
     4321 accgcacaac gggcaacggt tatggtagcg ggtcgagaac gccggctttc ttttcgcctt
     4381 cagcatcatc gtcaatctgg ccacagaaac cctcctttat ctttatctat cagttattga
     4441 acggcatgcc gaacatcttg aggagcgcct tcgccgcctt gtcatcacgg gcggaggtgc
     4501 atatcgtgac attcagcccc ttcacatttt cgaccttatt gaaatcgatc tcgggaaaaa
     4561 tcatgaaatc cttgatgccg agcgaatagt tacctctgcc gtcgaaggcc tttccggaaa
     4621 cgccgcggaa gtccttgacg cgcgggagcg cgaccgatat caagcggtca agaaaatcgt
     4681 acatgcggtc cttgcgaaga gaaacagagg tgcctatggg cgtcccttcg cgcagtttga
     4741 aggtggctat cgacttgcgc gccttgttga tgaccggtcg ctggccagcg ataagcgcca
     4801 gttccttcgc agcgtcctca agaagttttt tgttctgcgt ggcctccccg acacccatgt
     4861 tcaacacgat cctctcgata cgcgggaccg ccatagggtt ggtgatgccc agttccttct
     4921 ggagagcggg ccgtatctcc gtctgataat aggtgcgcat cctgctcaca tatttcggtc
     4981 gcttttcctc cggcgccttt tctttggccg cctctttggc cggcttcgcc gtcttctgtt
     5041 tctcgggcgc cgcctgctct ttgggcgtct ttgctttttt ctgtttttct tccatcatct
     5101 atctccgcct actctatggt ctcgccgcac gcacgacaga agcgcacccg ctttccgtcg
     5161 ctcaaaactt tgcggcggaa cgaagtggct ttggcacact tgggacagat cgcgcgcaca
     5221 ttcgagatgt ggatgggcat cgccttgtcc acgatccccg cctctcggtt ctgtccctgt
     5281 ttctgatgtt tcttgtacac attgacattc tctatcacca ccttctcggt atcggtgatg
     5341 acccgcaaga ctttgccggt cttccctttg tccttaccgg tgatgacgcg aaccgtatcg
     5401 ttctttttga tattcgccat tgtgctcatg gtgttctcct tacagtacct cgggggcgag
     5461 agatatgatc ttcatgaatc ccttgatcct gagttcgcgg gcgacggggc cgaacacgcg
     5521 ggtacccaac ggctcataat aatcgtcgag aatgaccacc gcgttgtcgt cgaaacggat
     5581 ataggaaccg tcctcccgtt tcaactcctt cgcctgtcgc acgatgaccg cgcgcttcac
     5641 ttcacctttc ttcaccttcc ccttcggctc ggcatccttg accgagacga cgatgacatc
     5701 accgatcccg gcgtatctcc gcttagagcc accgtacacc ttgatgcact gcacttcctt
     5761 tgcgccggag ttgtctgcaa cggccaatct ggtctgttcc tgtatcatgc tccgtctccc
     5821 tgcaacgcta tcctttcaac gatcagccag cgtttgcgct tactcaaagg ggcacattcg
     5881 ctgatgcgaa cacggtcgcc gatcttgcaa aggttctgtt cgtcatgtac caggtatttc
     5941 ttccgggtag tgatcatctt cttgtagagg ggatgcttgt agcggcgctg caccgagacc
     6001 gtgaccgtct tttccgcctt gtcgctcacc acgacgccga ccaatgtccg tttatggctt
     6061 cctgctttca tggggatcac ctcttatccg ttacggtatt ctgagaggga gcggcaagaa
     6121 aggtgtggac ccgggcgata tctttcttca ccaatctgat gcgggccgtg ttctgcaact
     6181 gccccatagc cttctggacg cggaggttga aatgttcttc ctcaagaacg agcaacagat
     6241 ccttcaactc ctcgcggctc ttcttcttca tttcgacctt tttcatcgta tctcgtcccc
     6301 cttcatgact atcttggtct ttatgggcaa cttataggaa gcgaggcgga gcgcctcttt
     6361 cgccgaagcg gcatcgagat cacccagctc gaacatgatg cgaccgggct tcaccacgca
     6421 gacccactcc tccggattac ccttgccctt gcccattctc gtctcaagcg gttttttcgt
     6481 gatcggcttg tcagggaaca cccggatcca catgcgacca cccttacggg cgatgcgcat
     6541 gatggcgaca cgggccgcct ctatctggcg gctggtgacc cacccgcact caagagcctt
     6601 gagaccgtac tcgccgaaat ctatggcgtt acaggcggtg gatatgcccg ccatgcgccc
     6661 cttcatcatt ttgcgatatt ttaccttatt cggcatcaac atggcgctca cctatcctgc
     6721 gaagaggttg atctcttttc tgccggtttt cgtctcgagt cgtagcgttt cgagacctcg
     6781 ctcttgtata tccagacctt gacaccgatc gtgccgtaag tggtgcgcgc gaccgcgtgc
     6841 ccgaaatcga tatcggcgcg cagggtatgg agcggtgtcc ggccttcgtg atagtgctcg
     6901 acccgggcga tctcgttgcc gccgagacga ccaccgacct gcacctttat accttcgatg
     6961 ccgctcttga tcgcattacc catggcccgc ttcatgacgc gacggaaatg ggcccgcttc
     7021 tcgatctgtt cggctatgct ctccgagacc agttgcgcgt tgatctccgg cttttttatc
     7081 tcttcgggct gcacgaacac ctcttgagca ttctgcgcga tcttgccgac gatctgcttt
     7141 attctttcga taccggcgcc ctgtttgccg acgaccagac cgggcttagc ggtacgaata
     7201 acgaccttga tcttttcgcc cgaccgttcg atggtcactg cggcgatacc ggaaaggtag
     7261 aactccttct tgatcgcttc gcggatctta aggtcctcat ggaaccactt cgcataattc
     7321 cggtgggagt accatacgga actccaggtc ctcgttatgc cgacgcgcag actgatggga
     7381 ttaacctttt gtcccaaggg acacctcctg ctctctcctc gttatccttt tttttctttt
     7441 ttctcaccga gctctatcgt gatatggctg gtgcgtttga cgatacggct cgcctgcccg
     7501 cgacatttgg tgatgaaccg tttccacatc ggcccttcgt cgaccgctat ggctaccacg
     7561 aacaaacgat cgggatcaag gccgaaattg ttgccggcgt tggctatagc cgaactcaac
     7621 agttttttaa gggcccccgc ggctcttcga ttggtgaaat cgagaatatt ctgggcatct
     7681 tcgacgcgtt ttccgcggat cacatcggcg acctcgcgca cccgtcgggg ggacatgcga
     7741 taatacttca gtgtcgcctt cacggtctta tcttccatct gagcctctcc aactcacaaa
     7801 aaattaagcg ggggtagtgt atgcatttcc ttcatttttg caatttattt tttcttctct
     7861 ccggcatggc ttttaaaggt ccttgtgggc gagaattcgc ccaatttgaa ccccaccatc
     7921 tcctcggtga tgaacaccgg aaggaacttg cgcccgttat gcacggcgaa ggtcagaccg
     7981 accatctcag gtacgatggt cgagcgacgc gaccaggtct ttatcggttt acggtcgctg
     8041 gagtcgatcg ccttcaccac tttcttccac aggtgcgcat caacgaacgg cccttttttt
     8101 actgaacgcg gcatggtgtc tccctcgctc ttgaattaat cggtgttgtt tacttctttc
     8161 ctcttcttcg cacgcggaaa cggtcggtcc gtttgttgtt gcgggtcttg tatcccttcg
     8221 tcggtttacc ccacggagat accggatgac gaccaccgga ggttttcccc tcgccgccgc
     8281 cgtgcgggtg gtcgaccgga ttcatcacaa cgccgcgaac cttgggacga cgacccagcc
     8341 aacgcgattt tcccgccttg ccgatacgga cattggacgc ctccgaattg ccgacctggc
     8401 ctatcgtcgc ccgacattcc agatggatta tgcgtatctc gccggaaggc atcctgaccg
     8461 tcgcgtagtc gccctccttg ccgagcaact gtataccgga ggccgcagat cggcctatct
     8521 gactgtcttt cccctgtttg gtctccacat tgtggatgac cgtaccgacc gggatatagc
     8581 gcaacttcat cgcgttaccg ggacgcacat caacatcctt gtcggccgaa acgatcgtgt
     8641 cgccgacctc aagtttttcc ggagcgatga tgtagcgttt ttcgccgtcc agataatgaa
     8701 tgagcgcgat atgcgcgttg cggttcgggt cgtattcgat cgccgcgacc tttcccggga
     8761 catttatctt gtcccgcttg aagtcgatga tgcgatagag gcgtttgttg ccgccgccac
     8821 gacgacgact ggtgatgcga ccgtaggcat tacgcccgcc cgacttcttc agcgaacgga
     8881 caagtgcctt ttcgggacgc ttctcggtca ggaccgtgtg atcggcaacc gacatgttcc
     8941 gtcttcccgc cgatgtgggg ttgtaagtct tgatacccat gattagcgct cccctgtgtt
     9001 atacgccttc gatgaaattg atactgtacc ccttctggag ggtcacatac gccttttttc
     9061 ggttgttccg cttgccggtc acccgtttca cctgcttgac ctttccgcgc acgataacgg
     9121 tattgacctc tttcactttt acatcgaaca ggctctcgac cgccgctttg atatcatcct
     9181 tttcagcgtc catggagacc tcgaagagat accccttctc ctctccacgg gacagcgctg
     9241 tcgatttctc cgtgatgatg ggccgcttga taatgccgaa gtatctcatt tttttcatga
     9301 cacatagacc tcgttaatgt atttagccgc ctcttcggtc atgaggatcg tcttatacgc
     9361 catgatgtcg tacagattca cctgagagaa atgcaacacc agatattccg gtatgttacg
     9421 tacgctcaaa gcgaatttct cctcacgatc cccggtaatg attatcgcct cggagagatc
     9481 ccatgtcttt ttcagaaggc tctttgcttc gcgggtcttg accgtatcga agtcgaatcc
     9541 cttgatcacg aaaaggctgc ctttttcaag gcgctccgaa agaaccgcct tcatggcaag
     9601 tcgccgcttc ttggccggta ccgtatagga gtaatcgcgc ggctgtggtc cgaggatgac
     9661 atagccgccc cgcatgatgc ccgcccgcat agtgccgcgg cgcgctcgac cggtaccctt
     9721 ttgacggaag ggtttcgaat tgccgccgga aacggtcgaa acggtcttcg tcgcatgggt
     9781 cccggaccgc caaccggccg tctgcatacg gaccacctcg tagaccggga cctgtcgttc
     9841 ctgccagttc acggcgaaca cttcatcatg cagcgttatg tcgccgacct ttttcttgtc
     9901 tcggtcaaat acatctatta ccggcatcat ctcctccgtc acttgctttt cttgatgtac
     9961 acgacgccgc ccgtcgcgcc cggaatggcg cctttcagca gaagcaggtt cttttcgggg
    10021 tatatcttca ccacctcgag attcttgatc gtcacctgtt cgttgccgag ccgacccgcc
    10081 atccgtttcc ctttccatat ctttcccgga tggacattct gcccgaccgc accgccctta
    10141 cggtgaaatt tcgagccgtg cgcatcgctg ccgccggaga aattccagcg tttcaccacg
    10201 ccggtgaaac cgcggccttt ggtgaagccg gtcaccttga ccttctcgcc gaccttgaaa
    10261 aggtccgcct ttaactcatc gccaagatta agaccctgca ggtccttctc gttgacgcga
    10321 aactctttta atcggcggaa tatccctttc ccggccgact tgaaatgccc cgcttccggc
    10381 ttccgaacat ttttactttt ctgttcgaaa ccgacctgga ccgcacaata gccgtccttt
    10441 tcggtgctct ttttctgtat gatgaagcag ggaccggcct cgatgacggt gacgggtatc
    10501 gagacgccgt cttcattgaa gatctgcgtc atcccgacct ttttcccaat aagtcctgca
    10561 ctcatggttt ctctccccgt ttaatttctc tttccaggac accctaactg gtctttatct
    10621 cgatctccac accggccgaa agatcggtct tcataagttc atccatcgtc tgcgtcgtgg
    10681 gattgagtat gtcgagtatc ctgcgatgga cacgcatctc gaattgctcg cgcgactttt
    10741 tatccacatg tggcgatcga agaacgcagt acttatggat cttggtcggc aacgggatcg
    10801 ggcccgcgat gcgcgcaccc gttcggataa cggtatcgat gatctccgac accgcgccat
    10861 ccaacagttt gtggtcgaac gccttgagct tgatcctgat cctgtcactc acgtcgtcac
    10921 ctcacagtta ctcgattatc tttgtcacca cgccggcgcc caccgtgcgg ccgccctccc
    10981 g
//
LOCUS       DAOQUE010000028        10929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_6745_length_10929_cov_4.297315, whole genome shotgun sequence.
ACCESSION   DAOQUE010000028 DAOQUE010000000
VERSION     DAOQUE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6745_length_10929_cov_4.297315"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2523
                     /locus_tag="PKH10_01715"
     CDS             <1..2523
                     /locus_tag="PKH10_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26872.1"
                     /translation="VEQGEVNGLATIGLPVSDIEGDGSWEQKKARAATAPRRGHVSAY
                     DTARGRLVVWGGETDGGLNSDTWEWDGTQWTKIDPPDPEGDGAPQQQELWAPSAMVYD
                     AARGEIILFHRMETWAWKGTSWVKRAGYGCTGPWCLECTSTTCPLLRYGQAMAYDTER
                     AKVVLYGGTWSSGEDKTWEWDGERWTKVTPTDPESDGNPPKIASGHAMVYDDLRGVVV
                     LFAAGTGWNETWEWNGVSWVKRTGSTCTTTSCYVCTSTTCPSPRYLHAMAYDGARGRV
                     VQFGGYYYDGWNQSCNDETWEWDGMVWTKRTPDDPGSDGNPDAMREHAMAYDTLHKKV
                     VLFGGYILYGGPVNILWEWDGASWAKKSPQAADVDGDPSARDTHEMAYDSDRGKVVLF
                     GGYSLSGSVNDTWEWDGSDWVKRAGDSCATGSCYVCAESTCPSARYQHAMAYDAERKR
                     TVLFGGYAFSCDGASNACRDTWEWNGTVWEKKSPSGDIPSGRYSHAMSYYEFDETHYG
                     VLLFAGQNASSVTNNETWEWNGTAWTQRAGDVCVGGSCYVCVIPTGMSDPAATCSKPR
                     EGHVMAFDAVRKKVVSFGGWTSAGMLTPDLWEWNGTIWVSKVPVVPKPSDNKYMSAMV
                     YDADREKMLLFGGQPQSASFDNETWEWSGLAWKQKAPADPEFDGIPPSRKEHAMAYDT
                     KRGRAVLFGGDNGGKNGDTWEWNGGTTARTGHIMEAAFGAAVTDETPFTVKKVTARFY
                     AGGVGYFFGVETPGAELKVWDEGRWQKVDDNAISPAALNSDPESGILEWTTESEAQIS
                     RLFFGEERRLNFAVTPMEANGTGAGKIATDYAEVIVRYTITE"
     gene            2539..5736
                     /locus_tag="PKH10_01720"
     CDS             2539..5736
                     /locus_tag="PKH10_01720"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNT26873.1"
                     /translation="MKRTCVLFAFLLFAVALRAAAPVAAVLEIEDKSGKFEKQVLWDA
                     TDYFRTKLVSGGKFVVVDRTKQAAKVKELAKKGKKESHGETVDAAFQIPLGKALAAQV
                     LIRPRITSFAGKFTFSVEIISIEKEAVIGGASAEFAGDESGLAAVMEKVAIEIGGKAQ
                     GSGAPVPTDDGAQIRAALFAKVTLDGKELSRDLLEGVIVEPLQTGNYHLVDIGTALKA
                     KASLFADEIAAGRMPKELTALEADALVSVKFDCAMATGAIGATKIQAYSCNLSTKMIR
                     VDNGEIAYAKQASIPGHGLSGEQAVRTLLQKKVPEAMVAQVTGSRGGWHKGGAWDLDI
                     WITRIKERRKAETVSELLAKLTGVASSKLIVFNTEYAKFRLTGSGASLAAVKKALFSD
                     PRFPLTVVHETERTIHARYNAAILFARPVDVLFSIEGGHAPDEAVAGTVKGHLLNVEY
                     LTARSVSRMTGARDAAIAKAAKAGERYLLLLTLRPANGTWRAAMELVDTASKEKLLTV
                     NGDGANLSAATAVAARRIEEAFKGALDKPFAGKFLKDAGEARSYAETEPVVIESFTVD
                     QILPALLPLYRTAGVGTLTLRNRGAASAGEAKVEFSVNDAVTGTVALGSLAAGETKKV
                     PVALNILPDTAQKRYAQMKAAVRYVAGEVHQRSDAYAPLLILDKDAIDWSEPRTLAAF
                     IDPQAAAVRAAATAATAAKRPDGFVTDKLARTAQIFSSLWRAPLKYVADPVPPGANTS
                     IDSVQHPAETLARMAGDCDDLTTLLAALLESVGIPAAVLVVPGHVLLAAETGLLAGGH
                     LLLGLPKELFVTIDGALYVPIEATAIGASFGAAWEKGAKAAREAGKDLTAFRIRSAWK
                     KYAAAVGASGKPVAPKIQTPDPAKLSATIPDLRKKSGGVPAFSVALYDRFSKGTALAA
                     DMKETANHALAAALMQWLTGKREEGAKGLERLCEQGSEEACYDIAALLFFEQKGQVAD
                     GAAVYEGMLSQLPSNIIDMMLDSGGFGLADEATDEAKLKRKLRETIEAARKKLEEKKK
                     TKNLDMIIKHVAGRKGDDLTGRPELFLLLFWDDAR"
     gene            5824..8418
                     /gene="mutS"
                     /locus_tag="PKH10_01725"
     CDS             5824..8418
                     /gene="mutS"
                     /locus_tag="PKH10_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNT26874.1"
                     /translation="MEPSLQKVLDTPVMEQYRTCKEQYPDAILFFRLGDFYEMFFDDA
                     VVASEILGLTLTSRHKEAGVPMAGIPYHSAENYIRKLLDAGRKVALCEQMEKPDKKKK
                     MIAREIVRVFTPGTVVEETALDEGRANYLLGVACPGNTAGLVWCDVSCGEVYFATVPR
                     EQVADTVRAIAPAETIGDGPVGETPIQPVDRDDLNDWVPSAQAADYLDHLAPELHDIA
                     VLSDAFRAVIWYLDRLYFGRFPPLRSPLPGRSAAHAALDTNTIANLEIERTLIGGNRQ
                     GSLLWAVDRTATVMGRRLLHRLLLQPLRDRAEIARRLDTVERFFSDDALRHRVREKLK
                     EIKDIERTLARILVKRGGPRDIVALCRSLSAATDLRDLVRSAAAELPSLAPLAADAAF
                     DRAIATRWERTFLSDAPLNWRDGGFVDPAHAPEVTRLHRIVHDSKGLLLELEEREKQA
                     TGIATLKLGYTKVFGYYLEITKRFAEKTPPHYIRKQTTVNGERYITPELKELEETILT
                     AREKLTEQELAILERTVDDIASQERAIQATALFVAWCDHFSACAEVARERRWERPVMT
                     DEEGIAIVEGRHPVVEQILGPGRYVPATLSVGDADGRLNIITGPNMGGKSTLMRTVAL
                     VTLLAQTGSFVPAREARIGVVDALFTRVGASDNLSRGESTFLVEMKETAFILRNATAR
                     SLIILDEIGRGTGTYDGISLARAIAEHLVRRVGATVLFATHYHLLTELADHLPGVRNY
                     HMTVREHRGGLQFLYQLAAGGSSRSFGVEVARLAGMPAEVVRDAEKGLRDLERTDREL
                     RLTQGGSMQLDIFALHSEMARQSSGHECLQKIRDLVSSNHPDRMSPKEALTLYYELFT
                     ILHSEDLS"
     gene            8415..9542
                     /gene="hisC"
                     /locus_tag="PKH10_01730"
     CDS             8415..9542
                     /gene="hisC"
                     /locus_tag="PKH10_01730"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNT26875.1"
                     /translation="MTVKVADYIERITPYRPGKPISEVERELGIYDTVKMASNENPLG
                     PSPKVMQAIKDDLKYIHLYPDGMAYYLKHAIMKHLARYHLSIDEVCVGNGSNEVIDIL
                     IRALVTPHEEILVSAQAFMIYELIPAAANIPVNLVPLTKDHRFDIEGYIERINDKTKM
                     VALVNPNNPTGTYYTKKEFENLLSNVPERCVVLVDEAYHEYVDAPDCPNAMDYRKDFE
                     NLIVCRTFSKAYGLSGMRLGYGITTKRIGETFRKVREPFNTSELAQVAGIAALEDEDY
                     VRRSVEMNRIGKEFFYAEFRRLELSFLPTQGNFILVHVGGGEGGGIDCYKALLQDGII
                     IRPVSNYGFPDWIRISIGLPEQNQRCLVALENYLRKEGRVR"
     gene            9640..>10929
                     /locus_tag="PKH10_01735"
     CDS             9640..>10929
                     /locus_tag="PKH10_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNT26876.1"
                     /translation="MTQNEVIICPSCDTKYRRPANIPPGGAQVKCKNCGTLFIVEGAA
                     PEITPSFATKFAGSLVRKSNGVIFKVSDLDDLEGRIARREVTPADETSSDGHEWRKLS
                     DLPELADLFSRAGLTGTEEATFFTDSLVRLPDGKIVRIADLSDLAGKIENGEVGEGDE
                     VSHNGIDWFPITSFDATRPLFTERHRKKTIAPGVMTSEEREPKKTMLISDATPPPSER
                     REQVVPAIKPAPAPAPAETQAARAETPSETGLREILAPKPAPAPEKKKVTDSIDDAEF
                     SKLFENDEEEKPRTPLKINMKIVVPVVTFIAIVIIVLLLVKGGGTDSDTAKKAVPDLT
                     EKSAPAPEPAPAPEPAPTPEPVPAPAPEPKKAEPAPEPVKSDLVKEQEAYLQRQKAAK
                     EAPKKEPIKRQAQQPSPKKERGGNAAAKALAKKGWAAI"
BASE COUNT         2265 a         3463 c         3495 g         1706 t
ORIGIN      
        1 gtggaacagg gcgaggtcaa cggccttgcc accatcggcc tgccggtatc ggacatcgag
       61 ggtgacggct cgtgggaaca gaaaaaagcc cgggcggcga ccgctccccg gcgcgggcat
      121 gtctcggcct atgatacggc gcgcggcaga ctggtcgtct gggggggaga gaccgacggc
      181 gggctgaact ccgacacctg ggaatgggac gggacgcagt ggaccaagat agatcctccc
      241 gacccggagg gggacggcgc tcctcagcaa caggaactct gggccccttc cgccatggtc
      301 tacgacgccg cccgcggaga gatcattctt ttccacagga tggagacctg ggcgtggaaa
      361 ggaacgagtt gggtcaaaag ggccgggtac ggctgcaccg gcccgtggtg tcttgagtgc
      421 acctccacca cctgccccct gttgcggtac gggcaggcga tggcctacga caccgaacgc
      481 gcaaaggtgg tcctgtacgg cgggacctgg tcgagcggcg aggataaaac atgggaatgg
      541 gacggtgaac gttggaccaa ggtcacgccg accgacccgg aaagcgacgg caacccgccg
      601 aaaatagcga gcggccacgc gatggtctat gacgacctgc gcggcgtggt ggtcctcttc
      661 gccgccggta ccggctggaa cgagacctgg gaatggaacg gcgtcagttg ggtcaaacgg
      721 accgggagca cctgtaccac caccagttgc tatgtctgca cctcgacgac ctgcccgtcg
      781 ccccggtacc ttcatgcgat ggcctacgac ggcgcccgcg ggagagtggt ccagttcggc
      841 ggctattact acgacggctg gaaccagtcc tgcaacgacg agacatggga atgggacgga
      901 atggtctgga ccaagcgaac gcccgacgat ccgggatccg acggcaaccc cgacgcgatg
      961 cgcgaacacg cgatggctta cgataccctg cacaagaaag tggtgctgtt cggcggctat
     1021 atcctgtacg gcggtcccgt caacatcctc tgggaatggg acggggcgag ttgggccaag
     1081 aagagtccgc aggccgccga tgtcgacggc gacccgtcgg cacgcgacac ccatgagatg
     1141 gcgtatgaca gtgaccgggg gaaagtggtg ctgttcggcg gatacagcct ctccggcagt
     1201 gtcaacgaca catgggaatg ggacggttcg gactgggtca aacgggcggg agacagttgc
     1261 gctaccggca gttgttatgt ctgcgccgaa agcacctgtc cttcggcccg ttatcagcac
     1321 gcgatggcat acgatgccga acgcaaacgg acggtcctct tcggcgggta cgcgttctcc
     1381 tgtgacggag cgagcaacgc ctgtcgggac acctgggaat ggaacgggac ggtctgggag
     1441 aaaaagagcc cttccgggga catcccttcc gggcggtaca gccacgcgat gtcgtactac
     1501 gaattcgacg agacccacta cggcgtgctc ctgttcgccg gacagaacgc gagttccgtg
     1561 acgaataacg agacctggga atggaacggc accgcgtgga cccagcgggc gggcgatgtc
     1621 tgcgtcgggg gcagttgcta tgtctgcgtc ataccgaccg gcatgtccga tcctgccgcc
     1681 acctgctcaa aaccgcgcga ggggcatgtg atggccttcg atgccgtgcg gaaaaaagtg
     1741 gtgtcattcg gcgggtggac gagcgccggt atgctcaccc ccgacctctg ggaatggaac
     1801 gggacgatct gggtatccaa ggtccccgtc gtccccaaac ccagcgacaa caagtacatg
     1861 tcggcgatgg tctatgacgc cgatcgcgag aagatgctgc tgttcggcgg acagccgcag
     1921 agcgcctcgt tcgacaacga gacctgggaa tggagcggtc ttgcgtggaa gcagaaggcg
     1981 cccgccgatc cggaattcga cgggatacct ccgtcgcgca aggaacacgc gatggcctat
     2041 gacaccaaac ggggacgtgc ggtcctgttc ggcggggaca acggcgggaa gaacggcgat
     2101 acctgggaat ggaacggcgg cacgacggcg cggacgggac acatcatgga ggcggcgttc
     2161 ggggcggccg tcaccgacga gacccccttc accgtgaaaa aggtcaccgc gcggttctat
     2221 gccggcggcg tcggctattt tttcggcgtc gagaccccgg gcgccgaact caaggtctgg
     2281 gatgaaggaa ggtggcagaa ggtggatgac aacgccatct cgcccgccgc cctgaacagc
     2341 gacccggaga gcgggatcct cgagtggacg accgaaagcg aagcgcagat atcccgcctg
     2401 ttcttcggcg aggaacggcg gctcaacttc gccgtgacgc cgatggaggc gaacggtacc
     2461 ggtgccggta agatagccac cgactacgcc gaggtgatag tgagatacac gataacagaa
     2521 tagataagag gagttgtcat gaaaagaacc tgcgttctct ttgcgttcct gctgttcgcc
     2581 gttgctctcc gcgccgccgc accggtcgcg gcggtcttgg agatcgagga caaatcgggc
     2641 aagttcgaaa agcaggtgct gtgggacgcc accgactatt tccggacgaa actcgtctcc
     2701 ggcggcaaat tcgtcgtggt ggacaggacc aaacaggcgg cgaaggtgaa ggaactggcc
     2761 aagaagggaa agaaagaatc gcacggcgag acggtggacg ccgccttcca gataccgctc
     2821 ggcaaggcgc tcgcggcaca ggtgctcatc cgaccgcgca tcaccagttt cgcggggaaa
     2881 ttcacctttt cggtcgagat catcagcatc gagaaggagg cggtcatcgg cggcgcatcg
     2941 gccgagttcg ccggtgatga atcggggctc gccgcagtga tggagaaggt agcgatcgag
     3001 ataggcggca aggcgcaggg gagcggtgcg cccgtcccga ccgacgacgg ggcccagata
     3061 cgggcggcgc tcttcgccaa agtgacgctc gacgggaagg aactgtcgcg cgaccttctc
     3121 gaaggggtca tcgtcgagcc gctgcagacg gggaactacc atctcgtcga tatcgggacg
     3181 gcgctcaagg cgaaggcatc gctgttcgcc gacgagatag cggcgggcag gatgccgaag
     3241 gagttgacgg cgctcgaggc cgacgcgctc gtgtcggtca aattcgactg cgcgatggcg
     3301 accggcgcca tcggcgccac gaagatacag gcctactcgt gcaacctgtc gaccaagatg
     3361 atacgcgtcg acaacggcga gatcgcctac gcgaagcagg cgagcatccc gggccacgga
     3421 ctgtcgggcg aacaggcggt ccggacgctg ctccagaaaa aagtgcccga ggcgatggtc
     3481 gcgcaggtga ccggttcgcg cggcggctgg cacaaaggcg gcgcgtggga cctcgatatc
     3541 tggatcaccc gcatcaaaga gcgacgcaag gccgaaaccg tctcggaact gcttgctaaa
     3601 ctgaccggcg tcgcctcgtc gaaactaatc gtcttcaaca ccgagtacgc caagttccgg
     3661 ctcaccggga gcggcgcgtc gctcgcggcg gtgaaaaagg cgctcttcag cgatccgcgg
     3721 ttcccgctca cggtggtgca cgagaccgaa cggacgatcc acgcccgcta caatgccgcc
     3781 atactttttg cgcgaccggt cgatgtcctc ttctccatcg agggggggca cgcccccgac
     3841 gaggcggtgg cgggtaccgt gaaaggacat ctgctcaatg tcgagtacct caccgcgcgg
     3901 tcggtgtcgc ggatgaccgg cgcgcgcgac gcggcgatcg caaaagcggc caaggcgggc
     3961 gaacgatacc tgctcctcct caccttgcgg cccgcgaacg gtacatggcg cgcggcgatg
     4021 gaactggtcg ataccgcatc gaaggagaaa ctgctcaccg tgaacggcga cggcgcgaac
     4081 ctttcggcgg cgacggcggt ggcggcgcgg cgcatcgagg aggcgttcaa gggcgcgctc
     4141 gacaaaccgt tcgcgggtaa attcctgaaa gacgcggggg aggcccgttc ctacgccgag
     4201 accgagccgg tggtgataga gagtttcacc gtcgatcaga tactcccggc gctcctgccg
     4261 ctctaccgca cggcgggggt cgggacgctc acgctccgca accgcggcgc ggcgtcggcg
     4321 ggcgaggcaa aggtcgaatt cagcgtgaac gacgcggtca ccggcacggt cgccctcggg
     4381 tccctagcag cgggcgaaac gaaaaaggtg ccggtcgcgc tcaacatcct tcctgatacg
     4441 gcgcagaaga gatacgccca gatgaaggcg gcggtccgct atgtggcggg cgaggtgcac
     4501 cagcggagcg acgcctacgc cccgctcctg atactcgata aggacgccat cgattggtcc
     4561 gagccgcgga cgctcgccgc cttcatcgac ccgcaggcgg cggcggtccg cgcggcggcg
     4621 accgcggcga cggcggccaa acggcccgac ggctttgtca ccgataaact ggcgcggacg
     4681 gcgcagatct tctcgtcgct gtggcgcgcg ccgctcaagt atgtcgccga cccggtgccg
     4741 cccggcgcga acaccagcat cgattcggtc cagcacccgg ccgagacgct cgcgcggatg
     4801 gcgggcgact gcgacgatct cacgacgctc cttgcggcgc tcctcgaatc ggtcggcatc
     4861 ccggcggcgg tgctcgtcgt gccgggccat gtcctgctcg cggccgagac ggggctcctc
     4921 gcggggggcc acctgctcct cggtctgccg aaggagctgt tcgtcacgat agacggggcg
     4981 ctctatgtcc cgatagaggc gaccgccatc ggcgcgtcgt tcggcgcggc atgggagaaa
     5041 ggggcgaagg cggcgcgcga agcgggaaaa gacctgaccg cgttccggat cagatcggcg
     5101 tggaaaaagt atgcggcggc ggtcggcgcg tcgggaaaac cggtcgcacc gaagatacag
     5161 acgcccgatc cggcgaaact ctccgcgacg atacccgacc tgcggaaaaa gagcggcggt
     5221 gtccccgctt tttcggtcgc gctctatgac agattctcga aggggaccgc gcttgccgcc
     5281 gatatgaagg agacggcgaa ccacgcgttg gcggcggcgc tcatgcagtg gctgaccggt
     5341 aagcgggaag agggagcgaa agggctggag cgtctctgcg aacagggaag cgaagaggcc
     5401 tgctacgata tcgcggcact cctgttcttc gagcagaaag ggcaggtcgc ggacggcgcg
     5461 gcggtctatg aggggatgct ctcccaactg ccgtcgaaca tcatcgacat gatgctcgac
     5521 agcggcgggt tcggacttgc cgacgaggcg accgacgagg cgaaactgaa acggaaactg
     5581 cgcgaaacga tcgaggcggc gcgcaaaaaa ctcgaagaga agaaaaagac caagaacctc
     5641 gacatgatca tcaagcatgt cgcggggcgc aagggggacg atctcaccgg gagaccggaa
     5701 ctgttcctgc tcctgttctg ggacgacgcg cgctgaatcg gtgaaaagac ttttttcctt
     5761 tgatttccgt ttattttttt gttagcgtcc ggtaagcgac acaccgtcac cgaccggagc
     5821 gcgatggaac cctctcttca aaaagtgctc gacacccccg tcatggagca gtaccgtacc
     5881 tgcaaggaac agtaccctga cgcgatactc ttcttccggc tcggcgattt ctacgaaatg
     5941 ttcttcgatg atgcggtcgt cgccagcgag atactcggac tcaccctcac ctcacggcac
     6001 aaggaggcgg gcgtcccgat ggcggggatc ccctaccaca gcgccgagaa ctacatccgc
     6061 aaactgctcg acgcggggag aaaggtcgcg ctctgcgagc agatggagaa gcccgacaag
     6121 aaaaagaaga tgatcgcgcg tgagatcgtc cgggtcttca cgccgggaac ggtggtcgaa
     6181 gagacggcgc tcgacgaggg gcgggccaac tatctgctcg gcgtcgcctg tccggggaac
     6241 accgcggggc tcgtctggtg cgatgtctcg tgcggcgagg tctatttcgc gacggtaccc
     6301 cgcgagcagg tcgccgacac cgtccgggcg atagcgcccg ccgagaccat cggcgacggg
     6361 ccggtcggcg aaacgccgat acagccagtg gaccgtgacg acctgaacga ctgggtcccc
     6421 tcggcgcaag cggccgatta cctcgaccat ctcgcgccgg aactgcacga catcgccgtc
     6481 ctgtccgatg cgttccgtgc ggtcatctgg tatctcgacc ggctctattt cggccggttc
     6541 cccccgctcc gctcgccgct cccgggccgg tcggccgccc acgcggcgct cgacaccaac
     6601 accatcgcga acctcgagat agagcggacg ctcatcggcg gcaaccggca ggggtcgctc
     6661 ctgtgggcgg tggaccgcac cgccaccgtg atggggcgac gactcctcca ccggctcctc
     6721 ctgcaaccgc tccgcgaccg cgccgagata gcgcggcggc tcgacacggt cgaacggttc
     6781 ttctcggacg atgcgctccg gcaccgggtc cgcgaaaaac tgaaggagat aaaggacatc
     6841 gagcggacgc tcgcccgtat cctcgtcaaa cggggcgggc cacgcgacat cgtcgccctc
     6901 tgccgatcgc tctcggcggc gaccgacctg cgcgacctcg tgcggagcgc cgcggccgag
     6961 ttgccttctc tcgccccgct cgcggcggac gccgcattcg accgtgccat cgccacgcga
     7021 tgggagcgca ccttcctttc cgacgcaccg ctcaactggc gcgacggcgg ctttgtcgat
     7081 cccgcacacg ctcccgaggt gacgcggctc caccgcatcg tccacgactc gaaggggctg
     7141 ctcctcgaac tcgaggaacg ggaaaagcag gccaccggca tcgcaacgct caaactcggc
     7201 tacaccaagg tgttcggcta ctatctcgag atcacgaagc gattcgccga aaagacaccg
     7261 ccccactata tccgcaagca gaccaccgtg aacggcgaac ggtacataac gccggaactc
     7321 aaggaacttg aggagacgat actcaccgcg cgcgagaagc tcaccgagca ggaactcgcg
     7381 atactcgaac ggaccgtgga cgacatagcg tcacaggagc gggccattca ggcgacggca
     7441 ctcttcgtcg cgtggtgcga ccatttctcg gcctgcgccg aggtcgcgcg cgaacggcgc
     7501 tgggaacggc ccgtcatgac cgacgaggag ggcatcgcca tcgtcgaggg acgccacccg
     7561 gtcgtcgaac agatactggg ccccggccgc tatgttcccg ccacgctgtc ggtcggcgac
     7621 gcggacggac ggctcaacat catcaccggc cccaacatgg gcggcaaatc gacgctcatg
     7681 cgcaccgtgg cgctcgtcac gctactcgcg cagaccgggt cgttcgtgcc ggcgcgcgag
     7741 gcccgcatcg gtgtggtgga cgcccttttc acgcgggtcg gcgcctccga caacctctcg
     7801 cgcggcgaat cgacctttct cgtcgagatg aaggagaccg ccttcatcct gcgcaacgcc
     7861 accgcccgca gtctcatcat cctcgacgag atcgggcgcg gcaccggcac ctacgacggc
     7921 atctcgctcg ctcgcgccat cgccgaacac ctcgtccggc gcgtcggcgc gacggtgttg
     7981 ttcgccaccc actaccacct gctcaccgaa ctcgccgacc acctgccggg cgtccgcaat
     8041 taccacatga ccgtccgcga acaccgcggc ggcctgcagt tcctctacca gttggcggct
     8101 ggcggatcga gccgcagttt cggcgtcgag gtggcgcggc tcgccggcat gccggccgag
     8161 gtggtgcgcg acgccgagaa ggggctccgc gacctcgaac ggaccgaccg cgaactgcgt
     8221 ctcacgcagg gcggatcgat gcaactcgac atcttcgcgc tccattccga gatggctcgg
     8281 caaagttccg gtcacgaatg tttgcaaaaa atacgcgacc tcgtatcatc gaaccatccc
     8341 gaccggatga gcccgaagga ggcgctgacc ttgtattacg aactttttac catattgcac
     8401 agcgaggacc tgtcatgacc gtaaaagtag ccgactacat cgaaaggatc actccctacc
     8461 gtcccggaaa accgatatcg gaggtcgagc gagagctcgg catctacgat accgtcaaga
     8521 tggcatcgaa cgagaatcct ctcggaccaa gtcccaaggt gatgcaggcg ataaaggacg
     8581 atctcaagta catccacctg tatcccgacg ggatggccta ctatctcaag catgccatca
     8641 tgaagcatct ggctaggtac catctctcca tcgacgaggt ctgcgtcggc aacggctcca
     8701 acgaggtcat cgacatactt atccgcgcac tggtgacccc tcatgaggag atactcgtct
     8761 ccgcgcaggc cttcatgatc tacgaactta tcccggcggc cgcgaacatc ccggtgaacc
     8821 tcgtgccgct caccaaggac catcggttcg acatcgaggg gtatatcgag cggatcaacg
     8881 acaagaccaa gatggtggcc ctcgtcaacc cgaacaaccc gaccggaacc tactacacca
     8941 agaaggaatt cgagaacctc ctctcgaatg tccccgaacg gtgcgtggtg cttgtcgacg
     9001 aggcctatca tgaatatgtg gacgcccccg actgcccgaa cgctatggat tatcgcaagg
     9061 atttcgagaa cctcatcgtt tgtcgtacct tctcgaaggc gtacggcctg agcggcatgc
     9121 ggctgggcta cgggatcacg accaaacgga tcggcgagac cttccgcaag gtgcgtgagc
     9181 cgttcaacac gagcgagctg gcacaggtcg cgggcatcgc ggcactcgag gacgaagact
     9241 atgtccggcg cagcgtcgag atgaaccgga tcggcaaaga gttcttctac gccgagttcc
     9301 gtcggctcga actctcattc ctgccgacac aggggaactt cattctcgtc catgtcggcg
     9361 gcggagaagg tggcggcatc gactgttaca aggctctttt gcaagacggc atcatcatcc
     9421 gcccggtcag caattacggt ttccccgact ggatacgcat ctcgataggt ctacccgaac
     9481 agaaccaacg gtgcctcgtc gcgctcgaga actaccttcg caaggagggg cgggtccgct
     9541 gatcacgctc ctcttcttcc tccttctttc cctcgttatt ttttgttgac ttgccggacg
     9601 ggcgcggcta aaatcggttc gcgataacag ggagcgccca tgacgcagaa cgaagtcatc
     9661 atctgtccct cctgcgacac gaagtatcgc cgcccggcga acatcccgcc cggaggggca
     9721 caggtcaaat gtaagaactg cggcaccctt ttcattgtcg agggcgcggc cccggagata
     9781 accccctcct ttgcaacgaa gttcgccggc tctctcgtcc gcaagtcgaa tggcgtcatc
     9841 ttcaaggtga gcgacctcga cgaccttgag gggcgcatcg cacggcgcga ggtgaccccc
     9901 gccgacgaga cctcctccga cggtcatgag tggcgaaagc tttccgatct tcctgaactc
     9961 gccgacctct tctcccgcgc cgggttgacc ggtaccgagg aggcgacctt tttcaccgac
    10021 tcgctcgtcc gcctgcccga cgggaagatc gtacggatcg ccgacctgtc cgatctcgcc
    10081 ggaaagatag agaacggcga ggtcggcgag ggcgacgagg tctcgcacaa cggcatagac
    10141 tggttcccga taaccagttt cgatgcgacc cgccccctgt tcaccgagcg gcaccgcaaa
    10201 aaaacgatcg cccccggcgt gatgacctcc gaggaacggg aaccgaaaaa gacgatgctg
    10261 atcagcgacg ctacgccccc gccctccgaa cgacgcgaac aggtcgttcc cgccattaaa
    10321 cccgcaccgg caccggcacc ggctgaaacc caggcggcgc gggccgaaac gccctccgag
    10381 accggcctgc gcgagatact cgctccgaaa ccggcgccgg cgcccgaaaa gaaaaaggtg
    10441 accgactcca tagacgatgc cgaattcagc aaactgttcg agaacgacga ggaggaaaaa
    10501 ccgagaacgc cgctcaagat caacatgaag atcgtcgtgc cggtcgtcac cttcatcgcc
    10561 atcgtgatca tcgttcttct cctcgtgaaa ggcggcggga ccgatagcga caccgccaag
    10621 aaagcggtcc ccgacctcac cgagaagagc gcacctgcac ctgagccggc accggcacct
    10681 gagccggcac cgacgcctga gccggtaccg gcccccgcgc ccgagccgaa aaaggcggaa
    10741 ccggcaccgg aaccggtgaa aagcgatctg gtgaaggagc aggaggcata cctgcaacgg
    10801 cagaaggcgg cgaaagaggc gcccaagaaa gagccgatca aacgacaggc acaacagccc
    10861 tcccccaaga aagaacgtgg agggaacgcc gccgcgaagg cgcttgccaa gaaagggtgg
    10921 gccgccata
//
LOCUS       DAOQUE010000029        10898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_6773_length_10898_cov_3.898921, whole genome shotgun sequence.
ACCESSION   DAOQUE010000029 DAOQUE010000000
VERSION     DAOQUE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6773_length_10898_cov_3.898921"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1875)
                     /gene="alaS"
                     /locus_tag="PKH10_01740"
     CDS             complement(<1..1875)
                     /gene="alaS"
                     /locus_tag="PKH10_01740"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNT26877.1"
                     /translation="MAHWTTDKARETFLNYFKERGHAVVASSPLLPAEDKTLLFTNAG
                     MNQFKNLFLGQEKRSYSRATTAQKCVRAGGKHNDLENVGFTARHHTFFEMLGNFSFGD
                     YFKKEAIAFAWELVTDKKYYGLDKERLYVTIYKDDDEAFEIWNKQEKVPAERIFRLGE
                     KDNFWAMGDTGPCGPCSEIHYDMWDNHTSREHFKGNEPDWDHGRFVEIWNLVFMQFDR
                     DAAGTLTPLPKPSIDTGMGLERVTAALNGVPSNYDIDVFVKLIGKLKELRGGAADPAQ
                     EASWRVIADHARATAFLIADSITPSNEGRGYVLRRIMRRAIRHGHKLGFTELFFHQAC
                     DGVIELMGHHYGELKKQREMILAIASEEEKRFRQTLDKGLALLNEEIKHAVSSGSSTI
                     SGDMTFVLYDTYGFPPDLTQTIIGEYNFTYDVSEYEAAMKQQKERGKASWSGNSEEKK
                     QAAAKMLLDEGIPATKFEGYDKSEMEGELVALFNSELDRVDSVRAGSNAYAILSPLLF
                     YAESGGQVGDKGTLEKGGTVAATVTDCVKLGEYRAAVVTAQTDLMVGTRFTQKLEEER
                     RSAIRRNHSATHLLHLALRKTLGDHAHQSGSLVTQERSYARFIASIRASCAALWSCVP
                     A"
     gene            2009..2083
                     /locus_tag="PKH10_01745"
     tRNA            2009..2083
                     /locus_tag="PKH10_01745"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2041..2043,aa:Gly,seq:ccc)
     gene            2104..5820
                     /locus_tag="PKH10_01750"
     CDS             2104..5820
                     /locus_tag="PKH10_01750"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNT26878.1"
                     /translation="MQRLRFLSVILLILLASLFIVRFSFYSVARTYLAAVHDIHLDIG
                     DTAICGLDCLAVPRLTVERPGAFRFEAEGITVRFGLRREEPFRVAPFLPYLGVRKARI
                     IVSDLASLTDDDPAPSDGALPYFPLGQVDVEDLSATLALPEGTLALTGISLRGDGRYT
                     LDIPTATYTAAGLRTAIAARADAEFVARGHDYSIVSVAGSGAGAEISLTATPGGFTGR
                     ASLALDQFIPRFDDRPATGTIVADLAGTLAGDAPRIEADVHATGLGWNGEFRIWDLAA
                     HLTADPRGLTVDHLALFNRDEPFLSLGGRMEWRDPVMRGKVTLWDLDFMNTMKRFQAP
                     CWVNMWISGTIDYDFSFKTLTARYRPRLTVRDFNLHDDGTLTILDLKDTYQVVGRGEI
                     RPERITLDEAEVTLSDDKTHIFLHDTWFDLDDDGKIHIMIHPRSRIDLSRVGAIAELP
                     VEGRGNIDMALTSFYESPRIVGHFKGNNCAVRGFEAGNCDLTVELKEFVLSFLGNSIV
                     HRSVRSEGSQVAIDFNKTPTDVSFLFNNMTGLLGDIGPTLRRELPPLGGRFIFNVAGR
                     YAGHLTSLTGHARADKVRWSETAIADQIAIAFVDDGDSLRLTKGVMKRGDSALSLSGT
                     MNRRTWALDAKATLDAFFAKDLDILKDVSFAAPQLTITTGGTLFAPTFTADIFLGQAA
                     YQQVAMGNFSATVAYEFESGGITASGNLGDAISFRGALRDLDTDTLTATAKVKDLIHK
                     QGDLFFKTSLDASWNDGELEARIQKIMVEKGKIFARNTRPFTLRGRWANLTIDPFVLD
                     GETLSGTVSGEIRNGVPHLDMTGTLFPAALAAFSGASGLHDITGNASFRLAVRDRAVS
                     GELSLQRLGFTVAPYDVPVADLEGTILFEGDRWRTERIRGLLGNGKTELRGSGSLTPS
                     FGGQATLSITNASLRHRLIGPFSFSAYLDIALSEGEKTPMVSGDIEIKNILYRDDLSF
                     DNELVRFLSRPAEKTDGEGARFNLRLFGRNNLRVQNNLIKSDLAFDTRLGGTLAHPTL
                     NGVMQFGNGELIFKQNRFPLDRGLITFEPGERISPFLDIQGTTRIYARSKETEYKAVL
                     TISGPPDNLAIRLSTVPQLEETQAYSLLLWGDFFDPTQPGIENLALVAVTDIMGISEE
                     VKKNFRLSRFELLPRYSEIDYQTVLKIVAEKELYPFLVMTIESNPADPADQMIGFSYR
                     GSNFDLSLDWKYKNKLETNYGGLGLNLALRYLFE"
     gene            5824..8775
                     /locus_tag="PKH10_01755"
     CDS             5824..8775
                     /locus_tag="PKH10_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNT26879.1"
                     /translation="MRSLSLLILLCAAPLLADITVERIEWKSDLPLDDFARLIKIEPG
                     APLSVKSVRRTVRLLYATGRFEQVVVTLLPGPDPERRAVSIAATPLRFIEDITITGNG
                     ALSDNRIKLAGNLRRFSPFYESGLERIREEILATYRSEGYPDATLSIEARNRSAAEVD
                     ILLSIQEGERRRIKKILLTGEVLSSERRELVNDLTLSNAFAALTDEQVRTMQEQVEHY
                     YRGRGHLDTVIRHQTQDDGTVVFTVQRGPRYTLSVAGANSFSAETIRRVIQHIEGWHR
                     NNEAANDRVILFYQAAGFPDVTVRTARTVKKRGTFEETLITVSISEGPRRFIDGIVFS
                     GALEEKSEDIAAHIVAYVETRLDAEDLPPVSLDRTIVGGGYRDTDGARVTTLSRTNKD
                     RASFPDARYVVPAAYLPDIATQVVEFYKARGYLQAEVLGHALARDGDLLFLDIEIAEG
                     ERSLVNWVDISSGDPELDRELLSLVDARSGIPFTPAFRDEITAKISTRLRELGRSFSR
                     VTVETVADGAHVRLLFRVDDAFPVQTGEVVVSGNIITEGSVVRRLTRIATGDLLTSDL
                     LREARQDLLATGAFDTAELTIMDEDAPEHMKDIVITVIEAWRYRLEIGAGVATDEGGR
                     LFGSFEYKNVLGQLFSLRLSYKLAHKIPWFMNSAFEDYFLHDLSFFERFDRVVNGSIA
                     FPDLYFLPFSLAFQAEAFHIHDSRTASGLPYLLDKNGMLLSFYKRFGEHFVLWVNAEL
                     SRQDEEEYKELYDETTASSTVSLERSTRYLLSPEVEGWADYRNSPVSPDRGFKIGLRV
                     RNVSSLAGDNTQYTLLENYASVYLPLQYLRSTSGEYLPRDTVIWHSFLRYGALLLHDG
                     MLSADDTLKLGGSTSVRGFAQNSIIPADDTDGDPQGKYYLFLRNELRLKIQDHLYFVA
                     FFDAGNLWEDIRQVGVGDLLRFGTGGGFMYASPIGSIHLQAGVNPFPREDTTRDYRED
                     LWSFHFFISSF"
     gene            complement(8787..10556)
                     /locus_tag="PKH10_01760"
     CDS             complement(8787..10556)
                     /locus_tag="PKH10_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNT26880.1"
                     /translation="MTHAYPTKVLYIDMTKKSYKTVERPELFAQYLGGTGAAIKLLDE
                     HCPAGIDPYAPDAPIIFSVGPLTGLFPLASKTVAMFKSPLTGNLGESHAGGRAAQAIR
                     MAGYGAVVVTGASDLPVYVAIHGSTVHFRDATSIWGMKSLFTAGSIIRAGEPGAGVRS
                     IIRIGRGGENKVAFGSATTETYRHFGRLGMGAVMGSKNIKAFVISGERSIPVEDKKKY
                     RDLYDQIFKAAVGSPVMKKYHDLGTAGNVRTMQGLKAMPTRNLQSGSFEHAEAISGES
                     LAEKYLGRRLACAHCPVGCIHIAAVREPYENESYFYKTTMVGYDWELIYALGSMLGIG
                     DPDGMLLLIDEVEKVGVDSMSAGVCLAWATEALEKGIIGEKETLGLKLAWGDWKTYIK
                     ATEHLVNQPNDFYKALAKGSVHAASIYGGADFALAFGGNEMPGYPTGPASYLGFLLGT
                     RHSHLDNAGYSLDQNAIKSGKALTPPQIVDELIKEECWRQILTSLCVCLFARGVYTPE
                     VVRGSLEVAGYSLSDEEILALGKTIYRDKYAFKIKHGFDFDALPKPARIFETPSPTGP
                     FDLAFIDEGLAYAKKRIGALLAG"
     gene            complement(10553..10885)
                     /locus_tag="PKH10_01765"
     CDS             complement(10553..10885)
                     /locus_tag="PKH10_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT26881.1"
                     /translation="MSNSCIGVRSAGGMSKGFTVIVCRSCKNPPCARACPVDALEMRP
                     EGGVRLKAEKCIGCGACRNACILKAVFWNHETQKPLICVQCGICAKYCPHGVLELRKG
                     LEKEEVRS"
BASE COUNT         2034 a         3592 c         3112 g         2160 t
ORIGIN      
        1 cgcgggcacg caggaccaca aggctgcgca cgaggcgcgg atcgaggcga tgaaccgggc
       61 gtacgaccgc tcctgcgtca ccagcgaacc cgactggtgg gcatggtcgc cgagcgtttt
      121 gcggagcgcg aggtgcaaca ggtgggtcgc cgaatgattg cgccgtatcg cgcttctccg
      181 ttcctcctcg agtttctgcg tgaagcgcgt gcctaccatg agatcggtct gcgcagtgac
      241 caccgccgcg cggtattcgc ccagtttcac acaatcggtc accgtcgcgg cgaccgtccc
      301 gcctttctcg agcgtcccct tgtcgcccac ctgaccaccc gattcggcgt agaacaggag
      361 cggcgaaagt atcgcgtagg cgttcgaacc ggcacggacc gaatcgaccc ggtcgagttc
      421 gctgttgaag agcgccacga gttctccttc catctcgctt ttgtcgtagc cttcgaactt
      481 cgtcgcgggg atgccttcgt caagcagcat cttggccgcc gcctgtttct tctcctcgct
      541 gttgccgctc cacgacgctt tgccgcgctc tttctgctgt ttcatcgcgg cttcatattc
      601 agaaacatcg tatgtgaaat tatactcccc gatgatcgtc tgggtgaggt cgggcgggaa
      661 accgtaagta tcgtaaagaa cgaaggtcat gtcgcccgat attgtcgatg acccactgct
      721 gacagcatgt ttgatctctt catttaacag cgcgagcccc ttatcgagcg tctggcggaa
      781 ccgtttctcc tcctccgagg cgatggcgag tatcatctcg cgctgtttct tcagttcacc
      841 gtagtggtgc cccatgagtt cgatgacgcc gtcgcacgcc tggtggaaga acagttcggt
      901 gaagccgagt ttgtggccgt gccgtatcgc gcgacgcatg atgcggcgca ggacataccc
      961 gcgtccctcg ttggagggcg ttatcgagtc ggcgatgaga aaggcggtcg cccgcgcgtg
     1021 gtcggcgatg acgcgccaac tcgcctcctg cgccgggtcg gccgcgccgc cgcgcagttc
     1081 cttcaacttc ccgatgagtt tcacgaacac atcgatatcg tagttcgacg gtacgccgtt
     1141 aagcgccgca gtgacccgtt caagccccat cccggtgtct atcgacggct tcgggagcgg
     1201 cgtgagcgtc cccgccgcgt cgcggtcgaa ctgcatgaag acgaggttcc atatctcgac
     1261 gaaccggccg tgatcccagt cgggctcgtt ccctttgaaa tgttcgcgcg aggtgtggtt
     1321 atcccacatg tcgtagtgga tctcgctgca gggaccgcac gggccggtgt cgcccatcgc
     1381 ccagaagttg tccttctcgc cgagccggaa tatccgttcg gcgggaactt tttcctgctt
     1441 gttccatatc tcgaacgcct cgtcatcgtc cttgtagata gtgacataga ggcgctcctt
     1501 gtcgagcccg tagtacttct tgtcggtcac cagttcccac gcgaaggcga tcgcctcctt
     1561 tttgaagtaa tcgccgaagg agaagttgcc gagcatctcg aagaaggtgt ggtggcgcgc
     1621 cgtgaagccg acattctcga ggtcgttgtg cttgccgccg gcccgaacgc atttctgagc
     1681 ggtggtcgcc cggctgtacg accgtttctc ctgcccgagg aagaggttct tgaactggtt
     1741 catcccggcg ttggtgaaga gcagtgtctt gtcctcggcc ggcaacagcg gcgaactcgc
     1801 caccaccgcg tgtccccgtt ccttgaaata gttcaggaat gtctcgcgcg ccttgtcggt
     1861 cgtccagtgg gccatgatac gctccttttc tcggaccttg tcgtcggcat catacttgcg
     1921 gcgcgcactt tctcaagatt tacccggtcc cccggaacgg ccttccgaat ttgacttttc
     1981 ggtcttctcc gggtataagg atgcccgagc gggtgtaact cagtggtaga gtgctagctt
     2041 cccaagctgg aagtcgcggg ttcaagtccc gtcgcccgct ccagattttt tttgtttggt
     2101 gatgtgcaaa ggctacgatt tctctcagta atcttactta tcctccttgc ctccctgttc
     2161 atcgtccgtt tttcgttcta ttcggtcgcg cggacctacc tcgccgccgt tcatgacatt
     2221 catctcgaca tcggggacac cgcgatctgc ggactggatt gcctcgccgt cccacgcctg
     2281 accgtcgagc gccccggcgc gttccgtttc gaggccgagg gcatcaccgt ccggttcggg
     2341 ctacgccgcg aggagccgtt ccgcgtggcg cctttcctcc cctatctcgg cgtgcgaaaa
     2401 gcgcggatca tcgtcagcga cctcgcgtcg ctcaccgacg acgaccccgc cccctctgac
     2461 ggggcgctcc cctactttcc tctcggacag gtcgacgtcg aggacctgtc ggcgacactc
     2521 gcgctaccgg agggcacact tgcgctcacc gggatatcgc tgaggggcga cgggcgctac
     2581 accctcgaca tccccaccgc gacctatacc gccgcaggct tacgcaccgc gatagcggct
     2641 cgagcagacg cggaattcgt cgcccgcggc cacgactatt cgatcgtttc ggtcgcggga
     2701 tcgggagcgg gcgccgagat atcgctcacc gccaccccgg gtggcttcac cggccgtgcc
     2761 tcgctggcgc tcgaccagtt catcccgagg ttcgacgacc gcccggcgac gggcaccatc
     2821 gtcgccgacc tcgccggaac gctggccggc gacgctccgc ggatcgaggc cgatgtccac
     2881 gccaccggac tcggatggaa cggcgagttc cgcatctggg acctcgcggc gcatcttacc
     2941 gccgacccgc gcggactcac cgtggaccac ctcgcgctgt tcaaccgcga cgaaccgttc
     3001 ctgtctctcg gcggcaggat ggaatggcgc gacccggtga tgcgtggcaa agtgacgctc
     3061 tgggacctcg acttcatgaa tacgatgaag cgcttccaag ccccgtgctg ggtcaacatg
     3121 tggatatcgg ggaccataga ctacgacttt tccttcaaga cgctcaccgc gcggtaccga
     3181 ccgcggctca ccgtgcgcga cttcaacctg cacgacgacg gaacgctcac gatcctcgac
     3241 ctcaaggaca cctaccaggt cgtcggtcgc ggcgagatac gccccgagcg gataacgctc
     3301 gacgaggcgg aagtgaccct cagtgacgac aagacgcata tattcctgca cgatacctgg
     3361 ttcgacctcg acgacgacgg gaagatacac atcatgatcc acccccgatc gcggatagac
     3421 ctctcgcggg tcggggcgat cgccgaactg cccgttgagg ggcgcgggaa catcgacatg
     3481 gccctcacct cgttctatga aagcccgcgc atcgtcggtc atttcaaggg aaacaactgc
     3541 gcggtgcggg gcttcgaggc gggcaactgc gatctgacgg tggaactcaa ggagttcgtc
     3601 ctttcgttcc tcggcaacag catcgtccac cggtcggtcc gctccgaggg ctcgcaggtc
     3661 gcgatagatt tcaacaagac cccgaccgat gtctcgttcc tgttcaacaa catgaccggc
     3721 ctgctcggcg atatcggacc gaccctgcgg agggaactcc ctcccctcgg cgggagattc
     3781 atcttcaatg tcgcggggcg gtacgcggga cacctcacct cgctcaccgg ccacgcccga
     3841 gccgacaagg tacgatggag cgagaccgcg atcgccgatc agatcgctat tgccttcgtc
     3901 gacgatggcg acagcctgcg ccttacgaaa ggggtgatga aacggggcga cagcgcgcta
     3961 tcgctctccg gcaccatgaa ccggcgcacc tgggcgctcg acgccaaagc aacgcttgac
     4021 gcgttcttcg ccaaggacct cgatatcttg aaggatgtct cctttgccgc cccgcagttg
     4081 accataacga cgggcggcac gctctttgcg cccactttca ccgccgatat cttcctcggg
     4141 caagcggcgt atcagcaggt cgctatgggt aacttctccg cgacggtcgc ttacgaattc
     4201 gaaagcggcg gcataacggc gtcgggcaat ctcggtgatg cgatatcgtt ccgcggcgca
     4261 ctgcgcgacc tcgacacaga cacgctcacg gcaaccgcca aggtcaagga tctcattcac
     4321 aaacagggcg atcttttctt caagacctcg ctcgacgcct cttggaacga cggggaactc
     4381 gaggcccgca tacagaagat catggtcgag aaaggaaaga tattcgcccg caatacccgc
     4441 cccttcacgc tgcgcggtcg ctgggcgaac ctcacgatcg atccatttgt actcgacggc
     4501 gagaccctga gcggcaccgt aagcggtgag atacgcaacg gcgtcccgca cctcgatatg
     4561 accggtacgc tgttccccgc cgcgctcgcc gccttcagcg gagcctcggg gctccacgac
     4621 atcacgggca acgcctcctt ccggctcgcg gtccgcgatc gcgccgtatc gggcgaatta
     4681 tcgctccagc ggctcggctt cacggtcgcc ccctacgatg taccggtggc cgacctcgag
     4741 gggacgatcc ttttcgaggg ggaccgctgg cgaacagagc ggatccgcgg acttttgggg
     4801 aacggcaaaa cggaactccg cggctcgggc tccttgaccc cctcgttcgg tggccaggcg
     4861 accctttcca tcaccaacgc ctcgctgcgg caccggctca tcggcccctt ttctttttct
     4921 gcctaccttg acatcgcttt gtcggaaggt gaaaagacac ctatggtgag cggcgacatc
     4981 gagatcaaga acatccttta ccgtgacgat ctctcgttcg acaacgaact ggtgcggttc
     5041 ctttcccgcc ccgccgaaaa gaccgacggc gagggagcgc gattcaatct ccggctgttc
     5101 ggtcgcaaca atctgcgggt acagaacaac ctgataaaaa gcgacctcgc cttcgacacc
     5161 cgactcggcg gcacgcttgc tcatccgaca ctgaacggcg tcatgcagtt cggcaacggt
     5221 gaactcatct tcaagcagaa ccgcttcccg ctcgaccgcg ggcttatcac cttcgagccg
     5281 ggcgagcgca tctcgccgtt cctcgacata caggggacca cccgcatcta cgcccggtcg
     5341 aaggagaccg aatacaaggc ggtgctcacc atcagcggcc cgcccgacaa cctcgctata
     5401 cgactctcga ccgtgccgca acttgaagaa acacaggcct actccctcct cctctggggg
     5461 gatttcttcg atccgaccca gccgggtatc gagaatctcg cgctcgtcgc cgtcaccgac
     5521 atcatgggga tatcggagga ggtcaagaag aacttccggc tctcccgctt cgaacttcta
     5581 ccgcgctatt cagagataga ctaccaaacg gtcctcaaga tcgtcgccga gaaggaacta
     5641 taccccttcc tcgtcatgac gatagaatcg aatcccgccg accccgccga ccagatgatc
     5701 ggattctcct accggggatc gaacttcgat ctgtcgctcg actggaagta taagaacaaa
     5761 ctggaaacga actacggtgg gctcggattg aatctcgcgc tccgctacct gttcgagtaa
     5821 acgatgcgta gtctgtcgtt actcattctc ctatgtgcgg cgcctctgct cgccgacatc
     5881 acggtcgagc gcatcgagtg gaaaagcgac ctgccgctcg atgatttcgc ccggctcatc
     5941 aagatagagc cgggagcgcc gctttcggtc aaaagcgtcc ggcgcaccgt gcgcctcctc
     6001 tacgccaccg gccgtttcga acaggtcgtc gtcaccctgc tgcccggccc cgatccggaa
     6061 cgacgcgccg tctccatcgc cgcgacgccg ctccgcttca tagaggacat caccatcacc
     6121 ggcaacggtg cactctcgga taaccggata aagctagccg ggaatctgcg gcgattctcg
     6181 cctttttacg aatcgggact tgaacgtatc cgcgaggaga tcctcgcgac ctatcgatcc
     6241 gagggatacc ccgacgccac gctcagcatc gaggcgcgta atcgctccgc cgcggaggtg
     6301 gacatcctcc tctcgataca ggagggggaa cggcgccgga taaagaagat actgctcaca
     6361 ggagaggtcc tctcctccga acgacgcgaa ctggtcaacg atctcactct gtcgaacgcc
     6421 ttcgcggcgc tcaccgacga acaggtccgc accatgcagg agcaggtcga gcactactat
     6481 cgcggccgcg ggcatctcga taccgtcatc cggcatcaga cacaggatga cggcaccgtc
     6541 gtgttcacgg tacagcgcgg cccccgctat acgctctcgg tggccggcgc gaacagtttc
     6601 tccgccgaaa cgatccgtcg ggtcatccaa cacatcgaag gctggcaccg caacaacgag
     6661 gcggctaacg accgcgtcat actgttctat caagccgccg gattccccga tgtcacggtc
     6721 cggaccgcgc ggacggtcaa aaaacggggg accttcgagg aaacgctcat caccgtctcg
     6781 atctccgaag gaccgcgtcg tttcatcgac ggcatcgtat tcagcggtgc tctcgaggag
     6841 aagagcgagg acatcgccgc acatatcgtc gcctatgtgg aaacccgcct cgatgccgag
     6901 gatcttccgc cggtgtcgct cgaccgcact atcgtcggcg gcggataccg ggacaccgac
     6961 ggagcccgcg tcaccacgct ttcgcgcacc aataaagatc gcgcctcctt tcccgatgcc
     7021 cgctatgtcg taccggcggc ctatctgccc gacatcgcga cacaggtcgt cgagttctac
     7081 aaggcgcgcg gctatctgca ggccgaagtg ctcggccatg ccttggcacg cgacggtgat
     7141 ctgctctttc tcgatatcga gatagcggag ggcgaacgct ctctggtgaa ctgggtggac
     7201 atctcctccg gcgatccgga actcgaccga gagcttcttt ctctggtgga cgcccgatcc
     7261 ggcatcccct tcaccccggc gttccgcgac gagatcactg caaagatatc tacgcggctt
     7321 cgcgaactcg gtcgatcctt ctcccgcgtg acggttgaaa cggtcgccga cggtgcccat
     7381 gtgaggctcc tgttccgcgt cgatgacgcc ttccccgtgc agaccggcga ggtggtcgtg
     7441 agcgggaaca tcatcaccga aggatcggtc gtacggagac tcacccgtat cgccaccggc
     7501 gatcttctga cgagtgacct cctccgcgaa gcacggcagg acctgctcgc gacgggcgca
     7561 ttcgacaccg ccgaactcac catcatggac gaggacgccc ccgagcacat gaaggatatc
     7621 gtgatcacgg tcatcgaggc ttggcgctac cgcctcgaga tcggcgccgg cgtcgcgacc
     7681 gacgaaggcg ggcggctctt cggatcgttc gaatacaaga atgtgctcgg acaactcttc
     7741 tccctgcgtc tttcctacaa actcgcccac aagataccgt ggttcatgaa cagcgcgttc
     7801 gaggactatt tcctgcacga tctctcgttc ttcgagcggt tcgaccgcgt cgtgaacggc
     7861 tccatcgcct tccccgacct gtatttcctg cccttctcgc tcgcctttca ggccgaggcg
     7921 ttccacatcc atgactcgcg taccgcgagc gggctcccct acctgctgga taagaacggc
     7981 atgctgctct ccttctacaa acgattcggc gaacatttcg tcctctgggt gaatgccgaa
     8041 ctttcgcgac aggacgagga ggagtacaag gaactatacg acgagacgac cgcgagcagc
     8101 acggtctctc tcgaacggtc gacccgctac ctgctcagcc ccgaggtgga gggttgggcc
     8161 gactaccgca acagtccggt aagccccgac cgcgggttca agatcggcct gcgggtccgg
     8221 aatgtctctt cgcttgccgg cgataacact cagtacactc tgctcgagaa ctacgcatcg
     8281 gtctatctgc cgctccagta cctccgttcc acctccggcg aatacctacc gcgcgacacc
     8341 gttatctggc attcctttct ccgatacggc gcgctcctgc tgcacgacgg catgctgagc
     8401 gccgacgaca ccttgaaact cggaggatcg acctcggtgc gcggtttcgc gcagaactcc
     8461 atcatcccgg cggacgacac cgacggcgac ccgcagggca aatactacct gttcctgcgc
     8521 aacgaactgc gattgaagat acaggatcac ctgtactttg tggccttctt tgatgccggg
     8581 aacctgtggg aggacatccg gcaggtcggc gtgggggacc tgctccgctt cggcacgggc
     8641 ggcggtttca tgtacgcgtc cccgatcgga agcattcatc tgcaggcggg ggtgaaccct
     8701 ttccctcgcg aggacacgac gcgggactac cgcgaggacc tctggagctt ccatttcttc
     8761 atatcgtcgt tctgaccgac gcgacatcaa ccggcgagaa gagctcctat cctctttttc
     8821 gcgtaggcca gcccttcatc gatgaaggcg aggtcgaagg gaccggtcgg agagggcgtc
     8881 tcgaagatac gcgccggttt gggcagcgcg tcgaaatcga aaccatgctt tatcttgaag
     8941 gcgtacttgt cacggtagat ggtcttcccg agtgccagta tctcctcatc ggaaaggctg
     9001 tagccggcga cctccaacga tccgcggacc acctccgggg tataaacgcc ccgcgcgaac
     9061 aaacagacgc agaggctcgt gagtatctgc cgccagcatt cctccttgat cagttcgtcg
     9121 acgatctgcg gcggcgtcag cgccttaccg ctttttatcg cgttctggtc gagactgtag
     9181 ccggcgttgt cgaggtggct gtgacgcgtc ccgagcagga agccgagata actcgccgga
     9241 ccggtcggat accccggcat ctcgttgccg ccgaacgcaa gggcgaaatc ggcgccgccg
     9301 tagatcgatg ccgcatggac cgaacccttg gcgagcgcct tgtaaaaatc gttcggctga
     9361 ttcacgagat gctccgtcgc cttgatgtag gtcttccagt cgccccacgc cagtttgagc
     9421 ccgagcgtct ccttctcgcc gatgatcccc ttctccaacg cctcggtcgc ccacgcgagg
     9481 cagacacccg ccgacatcga atcgacaccg accttctcca cctcgtcgat gagcaggagc
     9541 atcccgtcgg gatcgccgat gccgagcatc gatccgagcg catagatgag ttcccaatcg
     9601 tagccgacca tcgtcgtctt gtagaagtag gattcgttct cgtagggctc gcggaccgcg
     9661 gcgatatgaa tgcaaccgac cggacagtgc gcgcaggcaa gccgacggcc caagtatttc
     9721 tcggcaagcg attcgcccga tatcgcctcg gcgtgttcaa atgatccgct ctgcagattg
     9781 cgcgtcggca tcgccttcaa gccctgcatg gtcctcacat tcccggcggt ccccagatcg
     9841 tggtatttct tcatgaccgg cgatccgacc gccgccttga atatctggtc gtacagatcg
     9901 cggtactttt tcttgtcctc gaccggtatc gaccgttcgc ccgatatgac gaacgccttg
     9961 atgttcttgc tccccatcac ggcccccatc ccgagccggc cgaagtggcg gtaggtctcg
    10021 gtggtcgccg agccgaaggc gaccttgttc tcgccgccgc ggccgatgcg aatgatggag
    10081 cggacacccg ctcccggttc gccggcgcgg atgatcgacc cagcggtgaa aaggctcttc
    10141 atcccccaga tcgaggtggc gtcgcggaag tggaccgtcg agccgtggat cgcgacatag
    10201 accggcagat cggaggcgcc ggtcacgacg acggcgccgt accccgccat ccgtatcgcc
    10261 tgcgccgcgc gaccaccggc atggctctcg ccgaggttac cggtgagcgg cgacttgaac
    10321 atcgcgaccg tcttcgatgc gagaggaaag agccccgtca acggaccgac cgagaagatg
    10381 atcggcgcgt cgggcgcata gggatcgatg cccgccggac agtgttcatc gagcaatttg
    10441 atcgccgcac cggtgccgcc gagatactgc gcgaatagtt ccggccgttc gaccgtcttg
    10501 taactctttt tggtcatatc gatgtaaagg accttcgtcg gataggcgtg tgtcatgacc
    10561 gcacctcctc tttctccaag cccttccgca actcgagcac cccgtgcggg cagtacttcg
    10621 cgcagatacc gcactggaca cagatgagcg gcttctgggt ctcgtgattc cagaagaccg
    10681 ctttgagtat gcaggcattc cggcatgcac cgcacccgat gcatttttcc gccttcaatc
    10741 ggaccccgcc ttcgggccgc atctcgagcg cgtcgaccgg acaggcccgg gcgcatggtg
    10801 gattcttgca ggaacggcag acgatgacgg tgaatccttt cgacatgccg cccgccgacc
    10861 tgacgccgat acatgagttc gacagcccgg cgacaccg
//
LOCUS       DAOQUE010000030        10613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_7003_length_10613_cov_4.181474, whole genome shotgun sequence.
ACCESSION   DAOQUE010000030 DAOQUE010000000
VERSION     DAOQUE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7003_length_10613_cov_4.181474"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PKH10_01770"
     CDS             complement(<1..801)
                     /locus_tag="PKH10_01770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNT26882.1"
                     /translation="MVAHMLNILFLLFLPFLMLGVMSRIKALAGGRKGPPLLQPFFDM
                     LKLLRKGAVVSRVTSWVFTVAPSLALAATLTALLLAPGVGRAQVISFDGDIVLCLYLL
                     GLAKFFQLISALDTGSAFEGMGAGREALFSVIVEPSLFLFAGTLALVSGATSFGALFT
                     LAQSGAWPAVAATTGTLLFFLLMLTEGCRIPVDDPNTHLELTMIHEVMILDNAGPDLA
                     FLQYAAALKMYLFALLTAALVLPVDLSPLPAAFALPAVALLCAALAGIV"
     gene            complement(801..2777)
                     /locus_tag="PKH10_01775"
     CDS             complement(801..2777)
                     /locus_tag="PKH10_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNT26883.1"
                     /translation="MTLFLAGILTLLAAGVFQAATPRPARIACFALLAVAGLLLAGAG
                     AMTTLMNGAESVLFAFPFPLETVTLELDALSAFFVLVISFGGIAGTIYGAGYLGGYPD
                     RPLRGHLFAFALFILSMLLVVTAREVLPFLFAWELMSLSSLVLVFFDRRNDEVRDATV
                     NYFVAMHVAFALILTGFLLFMTRSGSGEFSSFAAATADGPFAETALLILFAGFAIKAG
                     FIPFHSWLPKAHPAAPSHVSGLMSGVMIKTGIYGILRFTLIVGVPTTRLALIVLALAL
                     LSAVVGVLYALAQHDLKRLLAYHSVENIGIIGIGIGLAMLGLRIGNGPLILLGMAGAL
                     LHVLNHALFKGLLFYGAGAVYRAAHTRDIEKLGGLIGKMPATAALFILGSVAISGLPP
                     LNGFISEFVLYLAMFAGAPLAAGAFGAVVVLTAGSLALTGILALYCFTKACGIVFLGT
                     PRSDAADRAHDPGGAMLAGMALLALAILAVGLLPAPFFAAVATVAATFPFPGDAAAQA
                     APIVATLGTVSRVLVLFLSLFAFLYYMRSRLLARNGGVAADATWGCGYRHATARMQYT
                     ASSYVSPLMALTGRLLGLHTHLERPQGLFPARTAFRSHPHDLVDAYLIAPGAKLIRYL
                     FSLLRWVQSGDMAQYILYGVLFLAAALAWIMGGT"
     gene            complement(2774..3532)
                     /locus_tag="PKH10_01780"
     CDS             complement(2774..3532)
                     /locus_tag="PKH10_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HNT26884.1"
                     /translation="MVLRLLRARWRHGWQAVPDPVTAQVDPMFRGFPEIAKEGTLSAG
                     TIAACPGAALTTNPVSIDLGKCHLCGHCARAENSPVRLSSRHHLGQTRRDALVVTAGT
                     SFEEYHARAEKGSDEIRRLFGRSLKLRSVSAAGCNACEMELNATTNANFDMLRYGIDI
                     VASPRHADAVIVTGPVSVNMADALEDTWNATPAPKLLILMGACAISGGLFAASPALDR
                     RFFNDHTPDIYIPGCPPHPLTVVNAILNYLGRGI"
     gene            complement(3542..5062)
                     /locus_tag="PKH10_01785"
     CDS             complement(3542..5062)
                     /locus_tag="PKH10_01785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNT26885.1"
                     /translation="MNRLLLKDRAAIPFDDLPTVSYEAVGDIVAGSLFGGLRLVTLFG
                     VPEERDIVRLVALFADDAQGTLAVTGVRTSRGATLPSLTPALPAFHLFERQLWEEFGI
                     VPAGHPWLKPVRYPHDAHTPRRIEEGYPYFSMEGEETHEVAVGPVHAGVIEPGHFRFL
                     CDGERVNHLEIQLGYQHRGVEKLFRNGPLASKSTLAESVSGDAAVAHATAHAGLVEAL
                     GDTVIPDRAGLLRAIGLELERAGIHLGDLSALCNDIGYLPGTQMFAVMRTDIINTSLA
                     LCGCRFGRGLITPGGVNYDIDDRKKDRIARTVIRIRDNAGLLAAAVFDDQSVLARFEK
                     TGTVDTDTARAIGLVGPAGRASGLACDIRLTHPTDAYARHRFTKRPLRNGDGRVAVSG
                     DVYARAFIRHQEIQDSLRYVLELLGHLAVAKDGIAAPVAPPRPGMIAFSLTEGWRGET
                     LHAAVTGGEGETRRYHIKDASFHNWFGLALAVRDNGVSDFPLCNKSFNLSYCGFDL"
     gene            complement(5172..6383)
                     /gene="hydF"
                     /locus_tag="PKH10_01790"
     CDS             complement(5172..6383)
                     /gene="hydF"
                     /locus_tag="PKH10_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNT26886.1"
                     /translation="MKAQRGDSLHIGIFGRRNSGKSSLINAITKQEIAIVSAVPGTTT
                     DPVFKGMELLPVGPVMFIDTAGIDDTDGTLGEERVKRTERILAKTDLALLVVPADART
                     FAFEEQLIASFDKYRIDFVLVLNKADTAPTPELGKWLGDRPVTKVSALKKEGIEELKK
                     AIIATAPKEWEPPLLRDLFEPGDAVLLITPIDRGAPKGRMIMPQAKAIREVLDGHGIV
                     VDVTEYEVEAALAALKVRPVLAVTDSQAFEKVAPAVPADIPLTGFSVLSIRQKGELRP
                     MVEGVMAVKTLQPGDKVLIAESCTHHPMEDDIGRLKIPRWLSEYVGGPLDVHVVPGYD
                     YPDDLSTYKLVVHCGGCTLNRREVLRRQSIPAKISVPMTNYGVLIAFLRGAFPRALTP
                     FPDLYEEFKDR"
     gene            complement(6396..7484)
                     /gene="hydE"
                     /locus_tag="PKH10_01795"
     CDS             complement(6396..7484)
                     /gene="hydE"
                     /locus_tag="PKH10_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005976949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNT26887.1"
                     /translation="MRPDIERILAKDELGRDDLVALLDISDPDEESALFRRAYEVKRK
                     HVGTKVYFRGLIELSNLCVKNCFYCGIRRVNRAVKRYTLTEEEVLDAVRFAHAARYGS
                     VVIQSGERQDEEFVSFIERLVKRVKEVSHNELGITLSLGEQSESTYRRWFDAGAHRYL
                     LRIETSNATLYKTLHPVDHDYEARLEALRTLYRIGYQVGTGVMIGLPGQTTEDLADDI
                     AFFKKMDVAMIGMGPFIPHHETPLAGSIPDWKEQKELQLRRALRMIAAVRIHLKDVNI
                     ASTTALQAIDHVGREMGLLAGANIIMPNLTDTEYRTGYQLYDDKPCMDENATMCTACL
                     ERRITSIGETVGYGEWGDSPHFINAKKH"
     gene            7652..9034
                     /gene="hydG"
                     /locus_tag="PKH10_01800"
     CDS             7652..9034
                     /gene="hydG"
                     /locus_tag="PKH10_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNT26888.1"
                     /translation="MDTGFIDHGRIYDYLKNTTPPDAKELDEILAHAKELKGISFAEA
                     ERLLMVEDDAHLDKIAETARFIKEEIYGKRLVLFAPLYVSNLCNNDCLYCAFKKSNKS
                     IPRRTLTFDELRREVQTLVQQGHKRILLVSGEGLGMNALDYTVKAIETIYSVKMGKGE
                     IRRINVNIAALPVEGFKRLKAARIGTYQLFQETYHAETYKTMHPSGPKSNYLYHLTAM
                     DRAMEAGIDDVGVGILFGLEDYRFEIMALLQHIRHLEERFGTGVHTISIPRLEPATGS
                     DVSVNPPHAVSDRDFRKIISILRITVPYTGMILSTREDAKMRHEALSLGISQISAGSR
                     TNPGGYSESDSAEQFSLGDHRSLEEVIRDIATMGYIPSFCTGCYRLGRTGQDFMDLAK
                     PGLIKHYCLPNAITSFKEYLVNYATPETRKVGDALIQKMLNDVPEAALRAKTAASLKK
                     IEEGAVDVYV"
     gene            complement(9228..9425)
                     /locus_tag="PKH10_01805"
     CDS             complement(9228..9425)
                     /locus_tag="PKH10_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26889.1"
                     /translation="MVTKKIEDANTRYYIDLDLRKGVIIGWDLGQREQLSQELPNPAH
                     HRVFLTKGQYRKLEKKKTIKN"
     gene            complement(9447..10271)
                     /locus_tag="PKH10_01810"
     CDS             complement(9447..10271)
                     /locus_tag="PKH10_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HNT26890.1"
                     /translation="MTKISVMKREVAIISLNEEDYICITDIAKYKNPDMTDDLIRNWL
                     RNRNTIEFLGIWERLNNPAFNPVEFDGFRKQAGLNSFTLTPKQWIEKTGALGILSKPG
                     RYGGTYAHKDIAFEFASWISVEFKLYLIKEFQRLKEAEHKQLGWDIRRNLTKINYRIH
                     TDAIKENLIPEEITKEEASLVYATEADVLNMALFGMTAKIWREKNPDKKGNIRDYADV
                     SQLVCLSNLENLNAHFINDALSQSERLVKLNKIAIHQMKLLTEDRTVKKLEKKEKV"
     gene            10480..>10613
                     /locus_tag="PKH10_01815"
     CDS             10480..>10613
                     /locus_tag="PKH10_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010280731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNT26891.1"
                     /translation="MKAIVYTEYGPPEVLHLQEVPTPEPKPNEVRVRIHAASVNFGDL
                     "
BASE COUNT         2102 a         3255 c         3319 g         1937 t
ORIGIN      
        1 gacgatcccc gcgagagcgg cgcagagaag agcgacggcc gggagcgcga atgccgcagg
       61 gagcggggaa aggtcgacgg gcaggacgag cgccgcggtg agaagcgcga agagatacat
      121 cttgagcgcc gcggcgtatt gcaggaacgc gaggtcgggc cccgcgttgt cgagtatcat
      181 cacctcgtgg atcatcgtga gttcgagatg tgtgttcgga tcgtcgaccg gtatgcggca
      241 cccttcggtg agcatcagca ggaagaaaag cagcgtcccg gtcgtcgcgg cgaccgcggg
      301 ccacgcgccg ctctgggcga gcgtgaagag cgcgccgaag gaggtcgccc ccgaaacgag
      361 cgcgagcgtc ccggcgaaca ggaacagcga cggctcgacg atcaccgaga agagcgcctc
      421 gcgacccgct cccatcccct caaaggcgct cccggtgtca agcgcggaga tgagttggaa
      481 gaacttcgcg aggccgagaa gatagaggca cagcacgatg tcgccatcga acgatatcac
      541 ctgcgcgcgg ccgacgcccg gcgccagcag gagcgccgtc aaggtcgccg cgagcgcgag
      601 cgacggggcg acggtgaaga cccacgaggt gacgcggctg accaccgccc ccttgcgcag
      661 aagtttcagc atatcgaaga acggctggag gagcggcggc cccttgcggc cccccgcgag
      721 cgccttgatg cggctcatga cgccgagcat caggaacggc aggaacagga gaaagaggat
      781 gttcagcata tgcgccacca tcaggtgcct cccatgatcc acgcgagcgc cgccgcgagg
      841 aagagtacgc cgtagaggat gtactgcgcc atgtcaccgc tctgcaccca gcgcagcagc
      901 gagaagagat accggatgag tttcgcgccg ggggcgatga ggtacgcgtc gaccagatcg
      961 tgcggatgcg accggaacgc cgtccgcgcc gggaaaagtc cctgcggtcg ctcgagatgg
     1021 gtgtggaggc cgagcaggcg gcccgtcagc gccatgagcg gcgacacata cgacgaggcg
     1081 gtgtactgca tccgcgccgt cgcgtggcgg taaccgcagc cccacgtcgc gtcggcggcg
     1141 accccgccgt tacgggcaag aagccgcgag cgcatataat aaagaaacgc gaagagcgaa
     1201 aggaacagca ccagcacccg cgacaccgtc ccgagcgtcg cgacgatcgg cgccgcctgc
     1261 gcggcggcgt cgccggggaa cgggaaggtc gccgcgaccg tcgcgaccgc cgcgaagaag
     1321 ggagcgggaa ggagcccgac ggcgaggatg gcgagcgcga ggagcgccat cccggcgagc
     1381 atcgcacctc ccgggtcatg cgcccggtcg gcggcgtcgc tgcggggcgt gccgaggaag
     1441 acgatgccgc acgccttggt gaagcagtag agggccagta tcccggtgag cgcgagcgac
     1501 ccggcggtga gcaccacgac cgccccgaag gcgcccgccg ccagcggcgc gccggcgaac
     1561 atcgccagat agaggacgaa ttcgctgatg aacccgttga gcggcggcag gcccgagata
     1621 gcgaccgagc cgaggatgaa cagcgcggct gtcgccggca tcttcccgat gagtccgccg
     1681 agtttctcga tatcgcgggt gtgggccgcg cggtagacgg cgccggcgcc gtaaaagaga
     1741 agtcccttga agagggcgtg gttgaggaca tgcaggagcg caccggccat cccaaggagt
     1801 atcagcgggc cgttgccgat gcgcagaccg agcatcgcca gtccgatgcc gatgccgatg
     1861 atgccgatgt tctcgacgct gtggtaggcg agcagccgct tgagatcgtg ctgggcgagc
     1921 gcgtagagga caccgaccac cgccgaaagg agggcgagag cgagcacgat gagcgcgagc
     1981 cgtgtggtgg ggactccgac gatgagcgtg aagcgcagga tgccgtagat gccggtcttt
     2041 atcatcacgc ccgacatgag ccccgatacg tggctcggcg ccgccgggtg cgccttgggg
     2101 agccaactgt ggaacgggat gaaccccgcc tttatcgcga agccggcgaa gagtatcagg
     2161 agcgccgtct cggcgaacgg gccgtcggcg gtcgcggcgg cgaaactcga gaattcgccc
     2221 gaaccgctac gggtcatgaa cagcaaaaag ccggtgagga taagcgcgaa ggcgacatgc
     2281 atcgcgacga agtagttaac ggtggcgtcg cgcacctcgt cgttacgacg gtcgaaaaag
     2341 accagcacca gcgacgagag agacatcagt tcccacgcga agaggaacgg caggacctcg
     2401 cgcgcggtca ccaccagtag catcgatagg atgaaaagcg cgaacgcgaa caggtgtccg
     2461 cgcagcggcc ggtcggggta gccgccgaga tagccggcgc cgtatatcgt cccggcgatg
     2521 ccgccgaaag agatgaccag cacaaaaaag gcagacagcg cgtcgagttc aagcgtcacc
     2581 gtctccagcg ggaaaggaaa agcgaaaagc acgctttcgg cgccgttcat cagcgtcgtc
     2641 atcgcgccgg cccccgcgag gagcagcccg gccacggcga ggagggcgaa acaagcgatg
     2701 cgcgccggac gcggcgtcgc cgcctgaaat accccggccg ccagcagcgt cagtatgccc
     2761 gcgagaaaga gtgtcatatc ccgcgcccca aatagttgag gatcgcattg accacggtga
     2821 gcggatgggg cggacagccg ggaatgtaga tatcgggcgt gtgatcgttg aagaaacggc
     2881 ggtcgagcgc gggcgacgcg gcgaacagtc cccccgatat cgcgcaggct cccatcagga
     2941 tgagcagttt cggcgcgggg gtcgcgttcc aggtatcttc cagcgcatcg gccatgttga
     3001 cgctcaccgg cccggtgacg atcaccgcgt cggcgtggcg cggcgaggcg acgatgtcga
     3061 taccgtagcg cagcatgtcg aagttggcgt tggtggtggc gttcagttcc atctcgcagg
     3121 cgttgcagcc ggcggccgac accgaccgga gtttgagcga acggccgaac aggcgccgta
     3181 tctcgtcgct ccctttctcg gcgcgggcat gatattcctc gaaactcgta ccggcggtga
     3241 cgacgagcgc gtcgcgtcgc gtctggccga ggtgatggcg actcgagagc cgtaccggcg
     3301 aattctcggc gcgggcacag tgtccgcaga gatggcactt gccgagatct atcgaaacgg
     3361 gattcgtcgt cagcgccgcg ccggggcatg cggcgatcgt cccggccgac aatgtcccct
     3421 ctttcgcgat ctcgggaaaa ccgcggaaca tcgggtcgac ctgcgcggtc accgggtcgg
     3481 ggaccgcctg ccacccgtga cgccagcgcg ctctcaacag tctgaggacc accgtgcctc
     3541 cttacaggtc aaaaccgcag tatgaaaggt tgaaactctt gttgcagagc gggaaatcgg
     3601 aaacgccgtt gtcgcgcacc gcaagcgcga ggccgaacca gttgtggaac gacgcgtcct
     3661 tgatatgata gcgccgcgtc tccccttccc cgccggtcac cgccgcgtga agcgtctcgc
     3721 cgcgccaccc ttcggtgagc gagaaggcga tcatgccggg acgcggggga gcgaccggcg
     3781 ccgcgatgcc gtccttcgcc acggcaaggt gcccgagcag ttcgagcaca tagcgcagcg
     3841 aatcctgtat ctcctgatgg cggatgaagg cgcgggcata gacatcgccg ctcaccgcca
     3901 ctctcccgtc gccgttgcgg agcggccgct tggtgaaacg atgacgcgca taggcatcgg
     3961 tcggatgggt caggcggata tcgcaggcaa gccccgatgc ccggcccgcc gggccgacaa
     4021 gcccgatggc gcgggccgta tcggtgtcga ccgtgccggt cttctcgaac cgggcgagca
     4081 cgctctggtc gtcgaatacc gctgcggcga gcaagcccgc gttgtcgcgg atacggatga
     4141 ccgtgcgggc gatgcggtcc tttttccgat cgtcgatatc gtagttgaca ccgcccggcg
     4201 tgatgagacc gcggccgaaa cggcacccgc acagcgcgag cgaggtattg atgatgtcgg
     4261 tgcgcatcac cgcgaacatc tgcgtgccgg gaagatagcc gatgtcgttg cagagcgccg
     4321 agaggtcgcc gagatgtatc ccggcccgct ccagttcgag cccgatggcc cgcaaaagcc
     4381 cggcgcgatc gggtatcacc gtatcgccga gcgcctcgac gagccccgcg tgggcggtcg
     4441 cgtgagccac cgccgcgtcg ccgctcaccg attcggcgag cgtacttttc gacgcgagcg
     4501 gaccgttgcg gaaaagtttc tcgacgccgc gatgctgata accgagttgt atctcgagat
     4561 ggttgacccg ctcgccgtcg cagaggaacc ggaaatgccc cggctcgatg acgccggcat
     4621 gcaccggccc caccgcgacc tcgtgcgtct cttcaccctc catggaaaag tagggatacc
     4681 cctcttcgat gcgtcgcggc gtgtgggcgt cgtgcggata gcggaccggc ttgagccacg
     4741 gatgacccgc gggaacgatg ccgaattctt cccacagttg ccgctcgaag agatggaacg
     4801 ccggcagcgc cggggtgagc gagggaagcg tcgcgccgcg ggaggtgcgg acgccggtca
     4861 ccgcgagcgt cccctgcgcg tcgtcggcga acagcgcgac gagccgcacg atgtcccgtt
     4921 cctccggcac gccgaagagc gtcaccaagc gtaacccgcc gaacagagag ccggcaacga
     4981 tatcgcccac ggcttcatag gatacggtcg gcaggtcgtc gaaggggatg gcggcacgat
     5041 cttttaagag gaggcggttc atggccggca ctatagcacc gcgccccgcc ttgtcaagta
     5101 ctcacatctt attgatattg tttgatattt tatttaaccg acgcgccgcc cggaacaggc
     5161 gaccctctat ttcagcggtc tttgaactct tcgtagagat cggggaacgg ggtgagggca
     5221 cgagggaagg cgcctctgag gaaggcgatg aggacgccgt agttggtcat cggcacgctg
     5281 atcttcgccg gtatcgactg gcggcgcagc acctcgcgcc ggttgagggt acagccgccg
     5341 cagtggacga ccagtttata ggtggaaaga tcgtcgggat aatcgtagcc ggggacgaca
     5401 tgaacatcga gcggcccgcc gacatactcc gacagccagc gcggtatctt gagccggccg
     5461 atatcgtcct ccatcgggtg gtgggtgcaa ctctcggcga tgagcacttt gtcgcccggc
     5521 tgcaaggtct tcaccgccat gaccccttcg accatcggac gcaactcacc tttctgccgt
     5581 atcgagagta ccgagaaacc ggtgagcggg atatccgcgg ggaccgccgg agcgaccttc
     5641 tcgaacgcct gcgaatcggt gacggcgagc accggccgca ccttgagcgc cgcgagcgcc
     5701 gcctcgacct cgtattcagt gacatcgacc acgatcccat ggccgtcgag cacctcgcgg
     5761 atcgccttgg cctgcggcat gatcatccgc cctttcggcg cgccacggtc tatcggggtg
     5821 atgaggagca ccgcgtcgcc cggttcaaag aggtcacgca ggagcggcgg ctcccattcc
     5881 ttgggcgccg tggcgatgat cgctttttta agttcctcta tcccctcttt tttcagcgcc
     5941 gagaccttcg tcaccggacg gtcgcccagc cacttcccca gttcgggcgt cggggcggta
     6001 tcggccttat tgagcaccag cacgaagtcg atgcggtatt tgtcgaaact cgcgatgagc
     6061 tgttcttcga aggcgaaggt ccgggcgtcg gccgggacca ccagcagcgc gaggtcggtc
     6121 ttggcgagta tccgttcggt ccgcttcacg cgctcttcgc cgagcgttcc gtcggtgtcg
     6181 tcgataccgg cggtgtcgat gaacatcacc gggccgaccg gcagcagttc catccccttg
     6241 aagaccgggt cggtggtcgt ccccggcacc gccgaaacga tggcgatctc ctgtttggtg
     6301 atggcgttga tgagtgacga tttgcccgaa ttgcgccgcc cgaagatgcc gatgtggaga
     6361 ctgtcgccgc gttgtgcttt cattatcttc tccctctagt gtttcttcgc attgatgaaa
     6421 tgcggcgaat cgccccactc gccgtagccg accgtctcgc cgatgctcgt gatacgccgc
     6481 tcgaggcagg ccgtgcacat cgtcgcgttc tcgtccatgc agggtttgtc gtcatagagt
     6541 tggtaaccgg tgcgatattc ggtgtcggtg aggttcggca tgatgatgtt ggccccggcg
     6601 agcagtccca tctcgcgacc gacatggtct atcgcctgca gggcggtggt cgaggcgatg
     6661 ttgacatcct tcaggtggat gcggaccgcc gcgatcatcc ggagcgcccg gcggagttgc
     6721 agttccttct gttccttcca gtcggggatc gaaccggcga gcggcgtttc atggtgcggg
     6781 atgaaggggc ccatcccgat catcgccaca tccatcttct tgaaaaaggc gatatcgtcg
     6841 gcgagatctt cggtcgtctg ccccggcagt ccgatcatca cgccggtccc cacctgatag
     6901 ccgatccggt acagcgtccg gagtgcctcc agccgcgcct cgtagtcgtg atcgacgggg
     6961 tggagcgtct tgtagagcgt cgcgttactc gtctctatcc gcagcaggta gcggtgtgcc
     7021 ccggcgtcga accaccgccg gtaggtcgac tccgactgtt cgcccagcga cagcgtgatg
     7081 ccgagttcgt tatgcgacac ctccttcacc cgcttcacga gccgttcgat gaaggagacg
     7141 aactcctcgt cctgccgctc gccgctctgg atgacgaccg atccgtagcg cgccgcatgg
     7201 gcgaaccgga ccgcatcgag cacctcctcc tcggtcagcg tatagcgttt caccgcgcgg
     7261 ttgacgcgcc ggatgccgca gtaaaaacag ttcttgacgc agagattcga cagttcgatg
     7321 agcccgcgga aatagacctt cgtcccgaca tgcttccgct tcacctcgta ggcgcgccgg
     7381 aagagcgccg attcctcgtc ggggtcgctg atgtcgagga gcgcgacgag gtcgtcgcgg
     7441 cccagttcgt ccttcgcaag tatccgttcg atatcgggtc gcatggggcc tccgccgagg
     7501 gtgatcgggt atcagtatag ggtgaaaaaa caagatatca aggggtgttg cgtgaaaagc
     7561 acctaatatt tggacatcta aggttgattt ttacttttca ggggcatata ttagtgaacg
     7621 gtttgctgtc cgtatcttta ggaggcctct catggatacc ggtttcatcg accacggcag
     7681 gatctacgac taccttaaga acaccacgcc gcccgacgcg aaggaactgg acgagatact
     7741 cgcccatgcc aaggaactga aaggcatttc gttcgccgaa gcggaacggc tgctgatggt
     7801 ggaggatgac gcccatctcg acaagatcgc cgagacggcg cgcttcatca aagaggagat
     7861 ctacggcaag cggctcgtcc tcttcgcgcc gctctatgtg tcgaacctct gcaacaacga
     7921 ctgcctctac tgcgccttca agaagtccaa caagtcgata ccgcgccgga cgctcacctt
     7981 cgacgaactg cgccgcgagg tgcagacgct cgtccagcag ggacacaaac ggatactgct
     8041 cgtgtcgggc gaagggctcg ggatgaacgc gctcgactac accgtcaagg cgatagagac
     8101 gatttacagc gtgaaaatgg ggaaaggcga gatacggcgg atcaatgtca acatcgccgc
     8161 cctcccggtc gagggcttca aacggctcaa ggcggcccgc atcggcacct accaactctt
     8221 tcaggagacc taccacgccg agacctacaa gacgatgcac ccgtcggggc ccaagtcgaa
     8281 ctatctctac cacctcaccg cgatggaccg cgcgatggaa gcgggcatcg acgatgtcgg
     8341 cgtcgggatc ctcttcggcc ttgaggacta ccggttcgag atcatggcgc tgctccagca
     8401 tatccgccat ctcgaagaac ggttcggcac cggcgtccac accatatcca tcccgcgcct
     8461 cgaaccggcg accggctccg atgtgtcggt caatccgccc cacgccgtga gcgaccgcga
     8521 tttccgcaag atcatatcga tcctccgcat caccgtcccc tacaccggga tgatactctc
     8581 cacccgcgag gacgccaaga tgcgccacga ggcgctgtcg ctgggcatct cgcagatatc
     8641 ggccggttcg cgcaccaacc ccggcggcta ttccgaaagc gattcggcgg agcagttctc
     8701 gctgggcgat caccgcagtc tcgaagaggt catccgcgac atcgcgacga tgggctatat
     8761 cccgtcgttc tgcaccggct gttaccggct ggggcgcacc ggtcaggatt tcatggatct
     8821 cgccaagccg gggcttatca agcactactg tctccccaac gccatcacga gtttcaagga
     8881 atatctggtc aactacgcca cccccgagac ccgcaaggtc ggcgatgcgc tcatccagaa
     8941 gatgctcaat gatgtgccgg aggcggcact gcgggcgaag accgccgcct cgctcaagaa
     9001 gatagaggaa ggggcggtcg atgtgtatgt gtaggtaaaa ccaacaccat caccgtaggg
     9061 gcgaatcttg tattcgcccg cattatcagg gcgatcacga ggatcgcccc tacaggccgg
     9121 aaggtgtttc gccgacgctc tcacgaaaat aaaagaatct tttttgctcg atccggtatc
     9181 ttatgaggtg ataggtgttt tttcattgaa ggtcaacggg gtcacatcta atttttgatg
     9241 gttttcttct tctccaactt gcggtactgg cctttggtga ggaagacgcg gtgatgagcc
     9301 gggttgggca attcctgtga caactgctcc cgctgaccga gatcccaccc gatgatcacc
     9361 ccctttctca agtccagatc gatataatac cgcgtgttcg catcttcgat cttcttcgtc
     9421 accatcgcaa cctcccccgc ccggattcaa accttctctt ttttctccaa cttcttgacc
     9481 gtccggtctt ccgtcagcaa cttcatctga tgaatggcga tcttattgag cttcaccaac
     9541 cgctccgatt gagaaagagc gtcgttaatg aaatgggcgt tcagattctc aagatttgaa
     9601 agacagacca gttgggatac atccgcgtag tctcggatat tgcctttttt gtccggattc
     9661 ttctcccgcc atatttttgc cgtcatgccg aaaagcgcca tgttcaggac atcggcctcg
     9721 gtcgcgtaca cgagacttgc ttcttctttc gtgatctctt cgggtatcag gttttctttt
     9781 atcgcatcgg tgtgtatccg ataattgatc ttggtaaggt tgcgcctgat atcccatcca
     9841 agttgcttat gctccgcttc tttcaatcgc tggaactctt ttatcagata tagcttgaac
     9901 tcgaccgata tccatgaggc gaactcgaat gcgatatcct tgtgtgcata agtcccgccg
     9961 tagcgaccgg gtttggaaag aatacctaga gcaccggtct tctctatcca ttgttttggc
    10021 gtaagcgtga agctgttcag gcccgcctgt tttctaaacc cgtcgaattc gacgggatta
    10081 aaagcggggt tgttcaggcg ttcccaaatg ccgaggaact ctatggtgtt gcgattccgc
    10141 aaccagtttc gtatcaggtc gtcggtcatg tcgggattct tgtacttggc gatatcggtt
    10201 atacaaatat agtcctcttc attcaaggag ataatggcga cttctctttt catcacactg
    10261 atctttgtca tcttcgctcc cccctatcag gatcgtatga aatcgaaact tcttcctatc
    10321 gccgcgataa tacccccgcc aaaattttga agcaagaaat ttcgcggtac tgacctttgg
    10381 tgaggaagac gcggtgatgc accgagcccg attttctctt gacttcgcgc ggcgcggccg
    10441 tcattctgca caggtgaacc attccgggaa ggagcgccca tgaaagcgat cgtctacacc
    10501 gaatacggac cgcccgaggt cctgcacctg caagaggtcc ccacccccga accaaaaccc
    10561 aacgaggtcc gcgtccgcat ccacgccgca tcggtcaact tcggcgacct gtt
//
LOCUS       DAOQUE010000031        10534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_7069_length_10534_cov_4.145911, whole genome shotgun sequence.
ACCESSION   DAOQUE010000031 DAOQUE010000000
VERSION     DAOQUE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7069_length_10534_cov_4.145911"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="PKH10_01820"
     CDS             complement(<1..1718)
                     /locus_tag="PKH10_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26892.1"
                     /translation="MKKIFVVVIVSFTFNLFAITDGIRYCQHIDLGGYCTDITSTRFD
                     LSQYTWTGGTPQNDNVSSILVMSGWEAWVCKDANHYGDCMFLSAGTYNMTNYAGWNDQ
                     ISSITVQPTTFYEAWSYYGKREFGESGTAPYGFAYAYACLNFVGCKRLFLKYPDSISS
                     LSNYIVKKNQIRSIELDGNVKATLHRDGYSSKILFSSHKNFSISNSITQQYGYLNLTA
                     FDQTVDGIMISMAPSIGFFQSELRQDFSGYPYILDFYNAYNFDNTTIDDARHEAYEVN
                     FSLGVADPNYGSQTSMAQKVSYYQNDQYTQRYGDNYDLLAIAAHGTLDIMDTDPTVRN
                     HWVAINSDLYPEDSNPYWYYKKYGGILKGWYEELPNYVEGAYSDWSKYIGTKSTEWVL
                     DSSCGSFGNHIDQHHGNISWSEILDIYDGPSTENGILDNLHGYGGTRGDWFFFVDDGE
                     SLGEEFYLNLSQYSVSSAWVASFAHLWTYVSGVLMNRDARYLTKETCDCSSIFCVAAY
                     MNNDHFHLYGTTQPDKGSRDNYPYTCMRRNSSWYLYDGTPYDPYLKMSSSVPSEVDGG
                     VDETIY"
     gene            complement(1990..2766)
                     /locus_tag="PKH10_01825"
     CDS             complement(1990..2766)
                     /locus_tag="PKH10_01825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT26893.1"
                     /translation="MTVALVTGASSGIGRELLDRFAADRMDLIITARDETALKTAADD
                     LTAKYGVAVTVIPADLARPGEAHRLFAEVEKRGIAVEYLVNNAGFGNYGALHEADARK
                     MDDMVQVNVASLVTLTRLFLPGMVARKSGRILQVASVAAFQPGPLMAVYYATKAFVLS
                     FSEAVAEELTGTGVTMTCLCPGPVHTQFAARSNFNHSRVFQSHAAQASDVADYGYRAF
                     MRGEILAVHGFNNRLLVLAGRFIPRRLIVKMVRWMQEKRD"
     gene            2897..3751
                     /gene="amrB"
                     /locus_tag="PKH10_01830"
     CDS             2897..3751
                     /gene="amrB"
                     /locus_tag="PKH10_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNT26894.1"
                     /translation="MNAIRPAGFAGSWYEGDPAALGRAVDRLVAGATGLPYAVRGAVL
                     PHAGHAFSGRGIAPLFRNLPPAIRSVVILAPSHYARLPEDRIGYGAFAALDTPLGPVP
                     GLAAPFAGRAPFAAMDRAVAAEHSAEMFLPFIRRTLPDAAAAILLIPRLTDAERLFSF
                     ADAIAGATAGLGEDTIFLASSDFTHYGDRFGYAPFGTRDIDAVLAKVAAQDRRYAELI
                     AHHRYAEVFEARRRDEPTICGLDPALVLSRLMDLRGLRGEVAGYYTSCDIAGKSADFV
                     CYATILFH"
     gene            3765..4328
                     /gene="amrA"
                     /locus_tag="PKH10_01835"
     CDS             3765..4328
                     /gene="amrA"
                     /locus_tag="PKH10_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNT26895.1"
                     /translation="MDLTPERERDLLQLARERIEEKIFDRPSPAWERLKGEPWLAEKS
                     GLFVTLKKDGDLRGCIGAIVGERPLGDTVRDMAIQSAFFDPRFPPVEAEELPEIEIEL
                     SLLTPLAPVDSWRDIVIGRDGMLLSYRGRQALFLPQVAPEQGWDLETTLTHLARKAGL
                     PAEIWREPDCRFKTFRAIVFGEKEPRR"
     gene            4332..5318
                     /gene="amrS"
                     /locus_tag="PKH10_01840"
     CDS             4332..5318
                     /gene="amrS"
                     /locus_tag="PKH10_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNT26896.1"
                     /translation="MKLVCDLCAHRCELSDGARGRCGIRENCGGKVVTINYGRVIALA
                     VDPIEKKPLYHFLPGTATLSVALHGCNFTCSFCQNHEISQKETFSATAGYPLPPAELA
                     RQWETRPTPSVSFTYSEPLVWQDYLLDAAAILRPKGARIVMVTNGFFSDEAFDRLLPV
                     VDAFNIDIKGDDRFYRERCGGRLDPVLRGIERIAAAPGKVLEATTLVIEGVHTADGIV
                     ALGKQLAELGVRVWHLSAYHPAYRLSLPATSERFLLDICDRVRTEVLIPYVYTGNIRS
                     SEGDHTRCAACDLLLISRRGFSIGDNLIRGNCCPSCHRSVYGLFDDPQSIRK"
     gene            5731..6381
                     /locus_tag="PKH10_01845"
     CDS             5731..6381
                     /locus_tag="PKH10_01845"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT26897.1"
                     /translation="MKVRGVIFDMDGTLLDTMERIALVMNTVLERRSLPVHEMVGYRR
                     FVGDGIRMLVEKALPEGRRDEATVAQVIAEVKALYDGERLKGTPAYSGIPELLQELDR
                     RRIPKAILSNKEEKFTRAHAEVDLAHFTFDPIVGARPDLPLKPAPDGALLIAGRWGIP
                     PAGILFVGDMPADVLTARAAGMPCAAALWGFSDKEDLVAAGATVFIEHPLDVVRLL"
     gene            complement(6445..6981)
                     /locus_tag="PKH10_01850"
     CDS             complement(6445..6981)
                     /locus_tag="PKH10_01850"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNT26898.1"
                     /translation="MRKKTLLALICAAVGFGCASSGDLPPLKTVAKLDINRYLGKWHE
                     IARYDHRFQRGCVGSTAEYALLPDGRISVTNTCREEDDPNELRQSTGVAWVDDPAEPA
                     KLRVRFFWPFSAAYWVIALDDDYQWAVVGHPSRDYLWILSRTATMDEELYRKILALIT
                     EQRYDVGKLQKRPLVKGD"
     gene            complement(7020..7874)
                     /locus_tag="PKH10_01855"
     CDS             complement(7020..7874)
                     /locus_tag="PKH10_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HNT26899.1"
                     /translation="MSNIKLFENKKVRSVWNEEKKRWFFVVADVVQILTESVNVTDYI
                     KKLRIRDGELGKGWGQIVTPLLIPTEGGPQRLNCADAEGILRIIQSIPSPKAEPFKRW
                     LAKVGYERLEEIENPELAAGRMREIYKAKGYSDQWIEKRMRGIAVRDELTNEWKQRGI
                     KEQVEYAILTADISKATFGMTPSEYKDFKELGGPDNLRDHMNDLELIFTMLGEASTTE
                     IARNRDAKGLKENRKAAKQGGKIAGNARKELEMKSGKKVSTKTNYKELPEAEERKKAI
                     TDESEAKE"
     gene            complement(7899..8486)
                     /locus_tag="PKH10_01860"
     CDS             complement(7899..8486)
                     /locus_tag="PKH10_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT26900.1"
                     /translation="MKKLIALCVTLMMTTLLFGGEPMNETIKTIMNRKSVRAYKEEPV
                     AKEKLELIVKAGMAAPTAVDKRPWEFIVITDKALLKKLSDVLPYAKMAEKAGAAILVA
                     GDLDKQNGGRDSVYWIMDCSAAIENILLAVESLGLGAVWTAIHPNADRIEPVRKLIKM
                     PSHVVPLALIPIGVPTGAEKPKDKWNPKQVHWNGW"
     gene            complement(8483..9016)
                     /locus_tag="PKH10_01865"
     CDS             complement(8483..9016)
                     /locus_tag="PKH10_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNT26901.1"
                     /translation="MNPIARYLELRDRMDELSARLTALHGENITCREGCTACCVNFTV
                     FPVEFHAILSEMERDGVKPESIPFDTTATCGFLHEGLCRIYRYRPIICRTHGLPILYL
                     DDEGDAPEWRVSFCELNFTGEGSVEFTDETLLDIEEMNAELYHINREFVASLHEKNYH
                     ENARIALKDICQKPEER"
     gene            complement(9073..>10534)
                     /locus_tag="PKH10_01870"
     CDS             complement(9073..>10534)
                     /locus_tag="PKH10_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26902.1"
                     /translation="ITDFFEKIREDNYSAAEPLLNGPALSGIYTFSGLNCTDDDSYRC
                     AGTMVSNWAIVLVYRSGMVRGHDILLYRGFSFLRGETMIVPVSGLLFPQYPTVTISTI
                     TAEGDRMSANQTLPPEMIYVKGENATSVYIPSNTCNPYQNMAYEVFNSDSSVFAWDAD
                     KNEPTCVTGGVGGAFPGIDVDTFRLSTEDDVNLLEHFYLLSTAFDLTLSANQDAILAN
                     LVVVAADTQSASFDIPPEATDWPYDREKHHCSCRNVDDPDDAWCEGQPMYFLIKVENW
                     GDNFAQNVIVVDELDDALEYVPGSTEMTDRTDEMFWISIPDKQGEGNDAFPLSGEGYK
                     VADIMEYCNQASWSCPDSRLIRFKVVPQWGLPKNVIIHNIATIKEEGSDEALWYHTNR
                     NVPLNLRKGQCGAAPLCPELSMEDCIGDIVDNDDDGVADDESGDDTDYYYCNEPDASY
                     YDDASPDVDMAEDPDEVTTPDTDTKKEIGCGCSLVF"
BASE COUNT         2095 a         3029 c         3029 g         2381 t
ORIGIN      
        1 atataaatgg tttcgtctac tccaccgtcc acttccgacg gcacagaaga tgacattttc
       61 aaatagggat catatggagt accatcatac aagtaccagg aagaatttct cctcatacaa
      121 gtgtagggat aattgtctcg cgatcctttg tctggttggg ttgtcccgta taagtggaaa
      181 tggtcgttgt tcatgtaggc cgcaacacaa aatatacttg agcagtcaca tgtttctttc
      241 gtaagatatc ttgcatcgcg gttcataaga acaccgctta catatgtcca cagatgagca
      301 aaggaggcga cccatgcact tgaaacggaa tattgagaca gatttaaata aaactcttcg
      361 ccaagagatt ctccatcatc cacaaagaaa aaccaatctc ctcgggtacc accatatcca
      421 tgcaggttat caagaatacc gttttctgtt gaagggccat catagatgtc aagtatttcc
      481 gaccatgaaa tgttgccgtg atgttgatca atgtggttgc caaacgatcc gcagctacta
      541 tccaaaaccc attcagtact tttcgtacct atatatttcg accaatccga gtaggctcct
      601 tcaacgtaat taggaagttc ttcataccaa ccctttaata tgccgccgta ttttttgtag
      661 taccaataag ggttcgaatc ctccggatat agatctgaat taatagccac ccaatgattt
      721 ctgactgtcg ggtctgtgtc cattatatca agagtgccat gtgctgcaat tgcaagcaaa
      781 tcataattgt ccccgtatcg ttgggtgtat tgatcattct gatagtaaga caccttttga
      841 gccattgaag tttggcttcc gtagttcgga tcggcgactc ccaaactgaa attcacctca
      901 tacgcctcat gtcttgcatc atcaattgtc gtgttatcaa agttatatgc attataaaaa
      961 tcgagaatgt agggataacc cgaaaaatct tgccttaatt cggactgaaa aaaaccaata
     1021 gacggcgcca tagatatcat tatgccatcg acagtctggt caaaagctgt gagattaagg
     1081 taaccatact gttgtgtgat cgaattgctt atagaaaagt ttttgtgaga tgagaacaaa
     1141 attttcgacg agtagccatc cctgtgtaat gtcgccttaa catttccatc aagttctatg
     1201 gatcttattt gatttttctt cacaatgtaa ttcgatagac ttgagatcga gtccgggtac
     1261 ttcaaaaata gccttttgca cccaacaaaa tttaagcacg cataagcata ggcaaaccca
     1321 tacggcgcgg taccagattc accgaattct cgtttcccat agtagctcca tgcttcgtaa
     1381 aatgttgtcg gttgaacggt gatggagcta atttggtcat tccatccagc ataatttgtc
     1441 atgttatagg tgcctgcgga caaaaacatg caatccccgt agtgattagc atctttgcat
     1501 acccatgctt cccacccact cataaccaga attgatgaca cattatcatt ttgaggtgtt
     1561 ccaccagtcc aagtatactg gcttagatca aatcgagttg atgtaatgtc tgtgcagtat
     1621 ccccctaaat cgatatgttg acaatatcta atgccgtcag ttatcgcgaa aagattaaaa
     1681 gtaaaagata caatcaccac aacaaaaatc tttttcataa cttattcccc atattttcta
     1741 aggtaaaaag tccgtttgat aaattttgtg atacggaggc gaaggcccct gatggaatga
     1801 aaaaaaggca actttcttgt atgtggatat gctaccaccc atcaagtgct ttccgtcctt
     1861 ttcagaaact ctcccactga cacccataag atacggcgag cggcttaaag gattcaggtg
     1921 ctccgaaata tttaggtaac tatttggaat gaaaggcgaa taatatcgtc agatgcgacg
     1981 actgaacggt cagtcccgtt tttcctgcat ccagcggacc attttgacga tgagccggcg
     2041 cgggatgaag cgaccggcga ggacgaggag gcggttgttg aagccgtgga ccgcgagtat
     2101 ctcgccgcgc atgaaggcgc ggtagccgta gtcggcgaca tcggacgctt gcgccgcatg
     2161 actctggaac acccgggaat ggttgaagtt cgatcgcgcc gcgaactgcg tgtggaccgg
     2221 gccggggcag aggcaggtca tcgtgacgcc ggtgccggtc agttcctcgg cgaccgcctc
     2281 ggagaacgac aggacgaagg ccttggtggc gtagtagacc gccatgagcg gaccgggctg
     2341 gaacgcggcg accgaggcga cctgcaggat gcggccgctc ttccgcgcca ccatcccggg
     2401 gagaaagagc cgcgtgagcg tgacgagcga cgcgacattc acctgcacca tgtcgtccat
     2461 cttccgcgcg tcagcctcgt ggagcgcgcc gtagttgccg aacccggcat tgttgacgag
     2521 atactcgacg gcgatgccgc gcttttcgac ctcggcgaag agccggtgcg cctcgccggg
     2581 tctcgccaga tcggcgggga tgacggtgac ggcgacgccg tacttcgcgg tcaggtcgtc
     2641 cgccgccgtt ttcagcgccg tctcgtcgcg cgccgtgatg ataaggtcca tccggtcggc
     2701 ggcgaaccgg tcgagcagtt cgcggccgat gccgctcgaa gcgccggtga caagggccac
     2761 ggtcatgaga tcctccgttg cgtaagggag atccgattat ggtcgaaaac cgatgcatgt
     2821 caatcgaacg atcattcttg ctttttcatg gcggcggcgt ctaatgaggg gcggaatgtc
     2881 aggggcaggg acgagcatga acgcgatacg cccggcgggg ttcgccggca gttggtacga
     2941 aggcgatccg gcggcgctcg gtcgcgcggt ggaccggctg gtcgccggcg cgacggggtt
     3001 gccgtatgcc gtccgcggcg cggtcctgcc gcacgcgggg cacgccttct cggggcgcgg
     3061 catcgcgccg ctgttccgta atctgccgcc cgccatccgg tcggtcgtca tcctcgcgcc
     3121 gagccactac gcccgcctgc cggaggatcg catcgggtac ggcgcctttg ccgcgctcga
     3181 caccccgctc ggcccggtgc ccgggcttgc cgctccgttc gcggggcgcg cgccgttcgc
     3241 cgcgatggac cgcgcggtcg cggctgagca cagcgccgag atgttcctgc cgttcatccg
     3301 ccggaccctg cccgacgcgg cggcggcgat actgctgata ccgcgcctta ccgacgccga
     3361 acggctcttt tcgttcgccg acgcgatcgc cggggcgacc gcggggctcg gcgaggatac
     3421 gatcttcctc gcgtcgagcg atttcaccca ctacggcgac cggttcggct acgcgccgtt
     3481 cgggacgcgc gacatcgacg ccgtgcttgc gaaggtggcg gcgcaggacc ggcggtatgc
     3541 cgaactcatc gcgcaccacc ggtacgccga ggtattcgag gcgcgtcgtc gcgacgaacc
     3601 gaccatctgc gggctcgacc cggcgctcgt gctgtcgcgg ctcatggatc tgcgcggcct
     3661 gcgcggagag gtcgccggct actacacctc gtgcgatatc gccgggaaga gcgccgattt
     3721 cgtctgttac gccacgatac tgttccattg atagggaggc tacgatggac ctgacgcccg
     3781 aacgggaacg cgacctactg caacttgcgc gcgaacggat agaggagaag atattcgatc
     3841 gcccgtcgcc ggcatgggag cggctcaagg gggagccatg gcttgccgaa aagagcgggc
     3901 tgttcgtcac gcttaagaag gatggcgacc tgcgcggctg tatcggcgcc atcgtcggcg
     3961 agaggccgct cggcgatacg gtccgcgaca tggcgataca gtcggccttc ttcgacccgc
     4021 gcttcccgcc ggtcgaggcg gaggaactgc cggagataga gatagagcta tcgctgctca
     4081 cgccgctcgc gccggtcgac tcgtggcgcg acatcgtcat cggcagggat gggatgctgc
     4141 tctcttaccg cgggcggcag gcgctctttc tgccgcaggt ggcgcccgaa caggggtggg
     4201 acctcgaaac gacgctgacc catctcgccc gcaaagcggg gctccccgcc gagatatggc
     4261 gcgagcccga ctgtcgcttc aagaccttcc gggcgatcgt gttcggcgaa aaggagccgc
     4321 gccgataggc catgaagctc gtttgtgatc tctgcgccca ccgctgtgaa ctgtccgacg
     4381 gcgcgcgggg ccggtgcggc atccgcgaga actgcggcgg caaggtcgtg accatcaatt
     4441 acggcagggt catcgcgctc gcggtcgatc ccatcgagaa aaagccgctc taccatttcc
     4501 tccccggcac ggcgaccctc tcggtcgcgc tccacgggtg caacttcacc tgttcgttct
     4561 gccagaacca cgagatatcg cagaaagaga ccttttccgc gaccgccgga tatccgcttc
     4621 cgcccgccga actggcgcgg cagtgggaga cgcggccgac cccgagcgtc agtttcacct
     4681 acagcgagcc gctcgtctgg caggactatc tgctcgacgc ggcggcgata ctgaggccga
     4741 agggggcgcg catcgtgatg gtgaccaacg ggttcttctc cgatgaggcg ttcgatcgtc
     4801 tcctgccggt cgtcgatgcc ttcaacatag acatcaaagg ggacgaccgc ttctatcgcg
     4861 aacggtgcgg cggccgtctc gacccggtcc tgcgcggcat cgaacgcata gccgccgcgc
     4921 ccggtaaagt gctcgaggcg accacgcttg tcatcgaggg ggtccatacc gccgacggga
     4981 tcgtcgcgct cggcaagcaa ctcgccgaac tcggcgtgcg ggtgtggcac ctgtcggcct
     5041 accaccccgc ctaccgtctg tcgctcccgg cgacctccga gcggttcctg ctcgatatct
     5101 gcgaccgggt acgcaccgag gtgctcatcc cctatgtcta caccggtaac atccgctctt
     5161 cggaaggcga ccacacccgg tgcgccgcct gcgacctgct ccttatctcg cggcgcggct
     5221 tctccatcgg cgataacctg atccggggca actgttgtcc ctcctgtcac cgttcggtct
     5281 atggtctttt tgatgacccg caaagcatac gaaagtagac acaaagcatg ttttttaacg
     5341 attatgacag ataaagatta cactttcctc taaagatata aaaatccggc aaagacgaag
     5401 ataaaaaata tgtcaatata tatatattgt tataaaatat tcagttaaac ttgggatttt
     5461 tggaccgcac attgctgtta gtctagggtg tgatagggtt ctttatcagt tcgagtttca
     5521 acagggcgtg gcggttcttt caggttcctg tcaagtgacc ttcgggtcgt cttttgtcaa
     5581 caccaacaca ttctttggcc gtccttaacc ggcggtcccg caactcgttt caagaaaagg
     5641 gaacggccgc gcgtatatca cggcccccgc gagggggctt ttttttatct tccgttgaca
     5701 tcgggcgggt ggtcccgcta taactctccc atgaaagtgc gcggcgtgat attcgacatg
     5761 gacgggacgc tcctcgacac gatggagcgg atcgcgctcg tcatgaacac ggtgctcgaa
     5821 cggcgttctc tgccggtcca cgagatggtg ggataccgcc gcttcgtcgg cgacgggatc
     5881 cggatgctcg ttgagaaggc gttgccggaa ggccgccgcg acgaggcgac cgtggcgcag
     5941 gtcatcgccg aggtgaaggc gctctacgac ggcgagcggc tcaaggggac ccccgcctac
     6001 tccggcatac cggaactgct ccaagaactc gatcgccgcc gcatcccgaa agcgatactt
     6061 tcaaataagg aggaaaagtt cacgagagcc cacgccgagg tggaccttgc ccacttcacc
     6121 ttcgacccga tcgtcggggc gcgccccgac ctgccgctca aaccggcccc cgacggagcg
     6181 ctgcttatcg cgggacggtg gggcatcccg cccgccggga tcctgttcgt cggcgacatg
     6241 ccggccgatg tcctgacggc gcgggccgcc gggatgccgt gcgccgcggc gttgtgggga
     6301 ttttccgata aagaggacct cgtcgcggcg ggggcgacgg tgttcatcga gcatccgctg
     6361 gatgtggtga ggttgctctg aagtcccgcc ctgtcaaggg agggcagggt gggttttcct
     6421 cacgccggac aatgcaaaac cccctcaatc ccccttgaca aggggacgct tttgaagttt
     6481 ccccacatcg taccgctgct cggtgatgag ggcgagtatc ttccggtaga gttcttcgtc
     6541 catcgtcgcg gtgcgcgaca gtatccagag gtagtcgcga ctcgggtggc cgacgacggc
     6601 ccattggtag tcgtcgtcaa gtgcgatgac ccagtaggcg gcgctgaagg gccagaagaa
     6661 acggacgcgc agtttcgccg gctcggcggg gtcatcgacc cacgcgacgc cggtgctttg
     6721 gcgcagttcg ttcgggtcat cctcctcgcg gcaggtgttg gtgaccgaga tgcggccgtc
     6781 gggcaggagc gcatactcgg cggtcgagcc gacacacccg cgctggaacc ggtgatcgta
     6841 gcgcgcgatc tcgtgccact tgccgaggta gcggttgata tcgagtttcg ccaccgtctt
     6901 cagcggcggc agatcgcccg acgaggcgca gccgaacccg accgcggcgc agatgagggc
     6961 aagcagggtc ttttttctca taagggaacc tctatttttc tcacaacacc tttcgccggt
     7021 cactctttcg cttcgctttc atcggttatc gcctttttgc gctcttccgc ttcgggcagt
     7081 tctttgtagt tggtctttgt cgaaaccttc ttgcctgact tcatttccaa ctctttacgg
     7141 gcattcccgg cgatcttgcc tccctgcttg gccgccttgc ggttctcttt gagtcctttc
     7201 gcatcgcggt tccgcgctat ctcggtcgtc gatgcttcgc cgagcatcgt gaagatgagt
     7261 tcgagatcgt tcatgtggtc acgcagattg tcgggaccgc cgagttcctt gaaatcttta
     7321 tattcgctcg gcgtcatgcc gaaggtcgcc ttgctgatgt cggcggtgag tatggcgtac
     7381 tccacctgtt ccttgatgcc gcgctgtttc cactcgttgg tcagttcgtc gcggaccgcg
     7441 atgccgcgca tccgtttctc tatccactgg tcgctgtaac ccttcgcctt gtatatctcc
     7501 cgcatccggc cggcggcgag ttcggggttc tctatctctt cgagccgctc gtacccgacc
     7561 ttggcaagcc accgtttgaa cggttcggcc ttcggtgagg ggatcgactg gatgatgcgc
     7621 aggatgcctt cggcgtcggc acagttcagc cgttgcgggc caccttcggt cggtatcaaa
     7681 aggggggtga cgatttgtcc ccaccctttg cctaactcgc cgtcacgaat gcgcaatttc
     7741 ttgatgtagt cggtcacatt cacactttcg gtcagtatct gaacgacatc ggctaccacg
     7801 aagaaccagc gttttttctc ttcgttccag accgaccgga ctttcttgtt ctcaaagagt
     7861 ttgatgtttg acataggctc tttccccctc acgagatcct accacccgtt ccaatgcacc
     7921 tgcttagggt tccacttgtc tttcggcttt tcggcgccgg tcgggacgcc gatggggatg
     7981 agggcgagcg gcacgacatg cgacggcatt ttgatgagtt tgcgcaccgg ttcgatgcgg
     8041 tccgcgttcg ggtggatcgc cgtccagacg gcgccgagcc cgagcgactc gaccgcgaga
     8101 aggatattct ctatcgccgc tgagcaatcc atgatccagt agaccgaatc gcgcccgccg
     8161 ttctgcttat cgagatcgcc cgcgacgagt atcgccgccc ccgccttttc ggccatcttc
     8221 gcgtagggaa ggacatcgct gagtttcttg aggagcgcct tgtcggtgat gacgatgaat
     8281 tcccacggcc gcttatcgac cgcggtcggc gcggccatcc ccgcctttac gataagttcc
     8341 agtttttctt tggcgaccgg ctcctctttg taggcgcgga cgctcttgcg gttcatgatg
     8401 gtcttgatgg tctcgttcat cggttcacct ccgaaaagca gggtggtcat catcagcgtg
     8461 acgcacaggg cgataagttt cttcatcgtt cctccggttt ttggcagata tctttgagcg
     8521 ctatccgtgc attctcgtga tagttcttct cgtggagcga cgcgacgaac tcgcggttga
     8581 tgtggtagag ttcggcgttc atctcctcga tgtcgagtag ggtctcgtcg gtgaattcga
     8641 ccgatccctc gccggtgaag ttgagttcgc agaaggagac ccgccactcg ggcgcgtcgc
     8701 cttcgtcgtc gagatagagt atcggcaggc cgtgggtgcg gcagatgatc gggcggtagc
     8761 ggtagatgcg gcagagccct tcatgcagga acccgcaggt cgcggtggtg tcgaatggga
     8821 tgctttctgg tttcacgccg tcgcgctcca tctcgctcag tatcgcatgg aactcgaccg
     8881 ggaagacggt gaagttgaca caacaggcgg tgcagccctc gcggcaggtg atgttctctc
     8941 cgtgcagggc ggtcagtcgc gccgacagct catccatccg gtcgcgcagt tcgagatagc
     9001 gtgcgatggg gttcatgcga tcattgtcct ttttcatcgc gccattctac cggagtggtg
     9061 gggggaagtc aatcagaaga caagggaaca cccgcagccg atctcctttt tcgtgtcggt
     9121 gtcgggcgtg gtgacctcat cgggatcctc ggccatgtcg acgtcgggag aagcgtcatc
     9181 atagtacgaa gcatcgggct cgttacaata ataatagtcc gtgtcgtccc cgctctcgtc
     9241 atcagcgacc ccatcatcgt cattatcgac gatgtccccg atgcaatctt ccattgatag
     9301 ttcgggacac agcggcgcgg cgccgcactg tcccttgcgg aggttcaagg ggacattcct
     9361 gtttgtatgg taccagagcg cctcgtcgct tccctcttct ttgatcgtgg cgatgttatg
     9421 gatgatcaca tttttgggca gtccccattg gggaacgacc ttgaatcgga taaggcgcga
     9481 atccgggcag gaccaagaag cctggttgca gtactccatg atgtcggcga ccttataccc
     9541 ctctccggag agcgggaagg catcattccc ctcgccctgt ttatcgggga tggatatcca
     9601 gaacatctca tcggtccggt cggtcatctc ggtcgaaccg ggcacatact ccagagcgtc
     9661 gtcgagttcg tcgaccacga taacattctg ggcgaagttg tccccccaat tctcaacctt
     9721 tatgaggaaa tacatcggct gtccttcgca ccatgcatcg tccgggtcgt caacattccg
     9781 gcaggaacag tgatgtttct cacgatcgta cggccagtcg gtcgcctcgg gcggtatgtc
     9841 gaaactcgcg gactgcgtat cggccgccac gacgacgaga ttcgccaata tcgcgtcctg
     9901 attagccgag agggtcagat cgaacgcggt gctgagaagg taaaaatgct ccagcaggtt
     9961 cacatcgtcc tcggtcgaaa ggcgaaaggt gtcgacatcg atgccgggga acgcgccgcc
    10021 gacaccgccc gtcacacagg tcggttcgtt cttgtcggcg tcccacgcaa agacggagga
    10081 atccgaattg aatacctcgt aggccatgtt ctggtagggg ttgcatgtat tggaagggat
    10141 atagaccgag gtcgcgttct cccctttgac atagatcatt tcgggaggca gggtctggtt
    10201 cgcggacatc cggtccccct cggcggtgat ggtgctgatc gtgaccgtgg ggtattgggg
    10261 gaacagaagc ccgctcaccg gaacgatcat cgtttctccc cgcaggaagg aaaaaccccg
    10321 gtaaagcagg atatcgtgtc cccggaccat ccctgatcgg tacacgagca ctatcgccca
    10381 gttcgacacc atcgtgccgg cgcaccggta agagtcatca tcggtgcagt tgagtcccga
    10441 aaaggtgtag atccccgaaa gggcgggtcc attaagcaga ggctctgccg cgctgtagtt
    10501 gtcctctcgt atcttttcga aaaagtctgt gata
//
LOCUS       DAOQUE010000032        10391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_7195_length_10391_cov_4.223200, whole genome shotgun sequence.
ACCESSION   DAOQUE010000032 DAOQUE010000000
VERSION     DAOQUE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10391
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7195_length_10391_cov_4.223200"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="PKH10_01875"
     CDS             complement(<1..1510)
                     /locus_tag="PKH10_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT26903.1"
                     /translation="MDLKLFQHIVKRSPHFDILRIIRPTDIIRAVMETHPSLTDEERM
                     RAREKINKAIVLATAAHQGVLRESGEPYITHPYAVAYVLAKMGMTVECVIAGFLHDTV
                     EDATTTIEEIRQMFGDAVANLVDGVTKLSLANIGKEEKKAQAFQKLFVYAARDGIGIL
                     FVKLADRLQNMMTLDAVSPEKQQRVARETLQLYAPLAHRLGVYWMKEELESLSFYYCY
                     PEEWKQIDDYITTRFQQDPAKALETLQKKVRAVIVEHCPPVAGKIEKVYGRIKSYPSI
                     YRKTIKQNKTIGSLHDIVGIRVILDSPNRDDCYLAMAAIHSFPEFTVMNDRFKDYIAR
                     PKANDYQSIHTVVRYHEYSMEVQIRTRGMHRIAEEGDASHWAYKNEIAGNDKAMLWLK
                     KVLGDLPDVTANPMDFVHDIATAIPLEKISVFTPKGDLITLPEGATVLDFAYAIHGDL
                     GDTCVGGTVNGRKVPIYYCLSNKDEVAIETSKKQFPRHDWLTFVATHKAKTYI"
     gene            complement(1515..2156)
                     /gene="gmk"
                     /locus_tag="PKH10_01880"
     CDS             complement(1515..2156)
                     /gene="gmk"
                     /locus_tag="PKH10_01880"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNT26904.1"
                     /translation="MRKKTTTQGPAARRGSAFVISAPSGTGKTTLVTALRKRFPFLEQ
                     SVSCTTRPPRTGEKSGRDYLFLTEEEFRRRIDEGAFLEWAKVYGHYYGTPKDWLIDRI
                     ESGRQVVCAIDVQGAVAIKQLLPDDAVLIFVLPPSLEELRRRIKNRALDDEAAIERRL
                     DEAKAEIRRYVHYDHIVVNDDLERAASLLSGIVATELYCSTRGNRELIERMLG"
     gene            complement(2252..3130)
                     /locus_tag="PKH10_01885"
     CDS             complement(2252..3130)
                     /locus_tag="PKH10_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNT26905.1"
                     /translation="MSHGHDHGDTGHIGVAFFLNFSFALIEIVGGLLTNSIAILSDAL
                     HDLGDSLALALAWYLQKVSDRGRDRRYTYGYKRFSLLGALAGSVILIVGSVVIVFAAV
                     PRLLTPQPTVTWGMLALAVLGIIVNGAAVLRLKKGTSQNAKVVALHLLEDVLGWAAVL
                     VGAVVIHFTGWYWIDPLLSLGIAAFILVTALGALRSSAKILLQGIPEGVDLAAIEARL
                     TAIAGVASFHDLHLWSLDGERTILTVHLVLDGPVTPETACRVKRETTAAMEELGVGHC
                     TLELEGPDEPCARCCS"
     gene            complement(3140..4162)
                     /locus_tag="PKH10_01890"
     CDS             complement(3140..4162)
                     /locus_tag="PKH10_01890"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNT26906.1"
                     /translation="MRAALIGLPVSGKTTVFEALTGIAEAKKEENLGTIKVPDTRIDR
                     LNEIYKPKKKTYAEFVLTDFNIAATKDALLPSQVKNLIQKADLLIFVLRNFDSVMTAE
                     PKDPLAEYRKLKDELLLTDLITAEKRIEREAKEKKNPPEMPAIKKLHAILEKSTFPAP
                     DAFTADELAHMANYNFLTLKKRIALVNEPEGGAPLPAGLTEAIAADGVLSFAVCATLE
                     KDLAQMAAEEQAEFLKGYGLTEPARARFIRTAYETLGLISFLTAGEDEVRAWPIRKGT
                     PAVEAAGKIHSDIQRGFIRAETIHFDDFNKYGSEAECKKAGHYRLEGKEYVVKDGDII
                     NFRFNV"
     gene            complement(4179..5072)
                     /locus_tag="PKH10_01895"
     CDS             complement(4179..5072)
                     /locus_tag="PKH10_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT26907.1"
                     /translation="MKRSIIIGLLTGFFALVSCQTVPVTADYAKTYAEAYAKGFEDGF
                     NKAEKLGFAGDKYPYQEPYNDIGAVDKDSVAYPVQKNDLKKVKELLETGRPADEMDPN
                     GYSPLQLAARLGFLPIAEYLVSKGADVNFQSSDGYTPLKEAVTYRKTPMAEFLIKKGA
                     DVNFTDSSGASILYFAAQAGDQALVKLLIEKKATLEQKLVSGGWTALLVAASYGFHPI
                     VALLTEAGADVNARETTSGKTALFYVIPSGNATTVKMLLEKGARTDVADNNGDTPLLL
                     AKRMNLPAIIELFEKKATPGT"
     gene            complement(5102..5536)
                     /locus_tag="PKH10_01900"
     CDS             complement(5102..5536)
                     /locus_tag="PKH10_01900"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNT26908.1"
                     /translation="MLANIRKDLFEAKKTHDLVRSNLLSTLLGEAMAVGKNAGNRETT
                     PEEVMQIIRKFMKNIDETIAILTGDGKACDKEKAERTILEGYLPKQLTAEELAKVIDD
                     IVATLPEKSPKAMGQVMAKLKELYAGRYDGKLASQLVKEKLV"
     gene            complement(5555..7225)
                     /gene="metG"
                     /locus_tag="PKH10_01905"
     CDS             complement(5555..7225)
                     /gene="metG"
                     /locus_tag="PKH10_01905"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNT26909.1"
                     /translation="MRYLVTSALPYANGPIHLGHIAGAYLPADIYVRFLRNSGREVIY
                     ICGTDEHGVPITINAEQNGKTPPEVVAENHRIIEDAFRRLEMSFDIFSGTSTCPDHAK
                     LSQDFFLTLRKNGFISERDVDQFYCDHDAMFLPDRYVEGVCPHCGKGARGDQCDYCGN
                     ALNSLELKEPKCKLCAKTPVVKSSRHWFLDLDKFQKDLDIWLKGKPHWKANVKAFCEQ
                     WFQRGLEPRGITRDISWGVPVPIEGAKGKVLYVWFDAPIGYISFTIELFRNLGRTEDW
                     KKWWQGGKDVKLVHFIGKDNIVFHAIIWPAMLMGQKGDWKLPDEIPANEFLNLEGDKF
                     STSKNYAIWLHEFLNDFNSDVIRYYLAQIAPEAKDADFSLKGFQTANNSELADIYGNF
                     VNRILSFTRKFFDGTLGGDVTFTDAEKALWAEAKKESDIIKECYDTFKVRDAVFHLME
                     LGRIANKYVDTSAPWSLRKTDEKRCKAVLLTCLNLIDTLATAFEPITPAASRKVKKML
                     NLPETFDLNALCSEPMKPGHTIGEPAVLYPKIEDAAIEKWREKLGINS"
     gene            complement(7307..8680)
                     /locus_tag="PKH10_01910"
     CDS             complement(7307..8680)
                     /locus_tag="PKH10_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNT26910.1"
                     /translation="MSAVRLRLFKRGRLILSRLLRRFLFFTGRVDPARAVFLGYLSYV
                     LVGWALLSLPIARTVDHIAPIDNLFIAASAMSTTGLVSISVSDNYSLFGQLVILALIQ
                     ICGVGFMTFGSFIILSRRPDLPPARTEVTHAVFSLPRSFDVFRFIRGVVLFTVVIESV
                     GTLLLCWAFTAAGTPRPFYTAFFHSISAFCTAGFSTFNDSFSAFSGNFWINGIIALLS
                     YLGAIGFIVFIDYWRRFRGETTQVTLTSRIILGATVWMTIIGTSLLFLAEPTITARPP
                     VDRLLAAFFQCMTAMTTVGFNTIDLGALSKGSVLVLTLLMIIGASPSGTGGGVKSTSF
                     TAILGVVRSALRGEQKASFSGKVIPFERIWQAIANTTLYLILLTVGVYLLDLTETAPF
                     DKIIFEAASAIGTVGLSMGVTGTLTDLGKFIVVLLMIGGRIGPLTVGMALFVRKHEEP
                     ADSDLAV"
     regulatory      complement(8741..8874)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(8920..10296)
                     /locus_tag="PKH10_01915"
     CDS             complement(8920..10296)
                     /locus_tag="PKH10_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26911.1"
                     /translation="MEERRNLPEFENSPKKWRYNFTKSLFTFWKNVKMYGVENQRIDE
                     EIAYFKKTLDFFFAEKDEFSILFDGIDVKVDKVRIRGQRQDDKYFDDIYDLFLSLCLS
                     GFVFKKGVDDRQILGFFAVLAKYPVGREPKIQAYERVRADLPPLTHIEVFPYDPEESG
                     NLPIYTVPQAIRRAHRKLAAEYPDYAKMVKAAENIPLRTVERSVQDLISIAQNLSDPG
                     NYALLMLLAALPAYRGSVAGAAAAARTIFSILIALRLGLEPSEVKRTAIAAYFQYLSD
                     DPDTGYTVLSRMDEFSYTRIEAALNASARLTDLSEVGLMMEGTACGTVPSEILKVAAY
                     YDRVTRRWHDGPLLSRPEALRSMLKNAGAGAFRRDIVDTFVTIAGVYPCGSVLRTISS
                     NELVVSCGRFKNFTAEGPVFVLNPDLSVQERRMVKAENLIDVPDAAGAVLPPATLIQM
                     LEAFLKEQ"
BASE COUNT         1915 a         2983 c         3273 g         2220 t
ORIGIN      
        1 ggatgtaggt ctttgccttg tgggtcgcca cgaaggtgag ccagtcgtgg cgcgggaact
       61 gtttcttcga tgtctctatc gccacctcgt ccttattcga gaggcagtaa tagatgggga
      121 ccttgcgacc gttgaccgtc ccgccgacgc aggtgtcgcc gagatcgccg tgtatcgcgt
      181 aggcgaaatc gagcaccgtc gcaccctcgg ggagcgtgat gaggtcgccc ttgggggtaa
      241 agaccgatat cttctcgagc ggtatcgccg tggcgatgtc gtggacgaaa tccatggggt
      301 tcgcggtgac atcgggcagg tcgccgagca ctttcttgag ccacagcatc gccttgtcgt
      361 tgcccgcgat ctcgttcttg taagcccagt gggaggcgtc accttcctcg gcgatacggt
      421 gcatcccgcg ggtccgtatc tgcacctcca tcgaatattc gtggtaacgg accaccgtgt
      481 gtatcgactg ataatcgttc gccttgggtc gcgcgatgta gtccttaaat cggtcgttca
      541 tcacggtgaa ctcggggaac gaatggatgg ccgccatcgc gaggtagcag tcgtcgcggt
      601 tcggcgaatc gagtatcacg cggataccga caatgtcgtg cagggatccg atggtcttgt
      661 tctgtttgat ggtcttacgg tagatgctcg ggtaggactt gatgcggccg tacaccttct
      721 ctatctttcc cgccacgggc ggacaatgct cgacgatgac cgcgcgcacc ttcttctgca
      781 gggtttcgag cgccttggcc gggtcctgct ggaaccgcgt ggtgatgtag tcgtctatct
      841 gtttccattc ctcgggatag cagtagtaga aactgagcga ctcgagctcc tctttcatcc
      901 agtaaacgcc gagccgatgg gcgagcggcg cgtacagttg cagggtctcc cgcgcgaccc
      961 gttgctgttt ctcggggctc accgcgtcga gcgtcatcat gttctgaagg cggtcggcca
     1021 gtttgacgaa cagtatcccg atgccatccc gcgccgcgta gacgaagagt ttctggaacg
     1081 cctgtgcctt tttctcctcc ttgccgatgt tggcgagcga cagtttggtc acgccatcca
     1141 ccagattggc gaccgcgtcg ccgaacatct gccggatctc ttcgatggtg gtggtcgcgt
     1201 cctctaccgt gtcgtgcagg aacccggcga tgacgcattc aacggtcatc cccatcttgg
     1261 cgagtacata ggcgacggca tagggatggg tgatgtaggg ctcgccgctc tcgcgcagga
     1321 ccccttgatg cgcggcggtc gcgaggacga tggccttgtt gatcttttcc cgcgccctca
     1381 tccgctcttc gtcggtcagg gaggggtgcg tttccatgac ggcgcggatg atgtcggtgg
     1441 ggcggatgat ccgcaggatg tcgaagtggg gagatctctt gacgatgtgc tggaacagtt
     1501 tcaggtccat ggtctcagcc cagcatccgc tctatcagtt cgcggttccc gcgcgtggag
     1561 cagtacagtt cggtcgcgac gatgcccgag agcagcgaag cggcgcgttc gaggtcgtca
     1621 ttcacgacga tgtggtcgta gtggacatag cgtcgtatct cggcctttgc ctcgtcgagc
     1681 cgccgctcga tggccgcctc gtcgtcgagc gcgcggttct tgatgcgccg ccgcaactcc
     1741 tcgagcgacg ggggaaggac gaatatgagc acggcgtcgt cggggagcag ttgtttgatg
     1801 gcgaccgccc cctgcacatc gattgcgcag acgacctgcc gcccgctctc tatccgatcg
     1861 atgagccaat ccttcggcgt cccgtagtag tggccgtaca ccttcgccca ttcgaggaag
     1921 gcgccctcgt cgatgcgtcg ccggaactcc tcctcggtca ggaacaggta gtcgcggccg
     1981 ctcttctcgc cggtgcgcgg cgggcgggtg gtgcaggaga ccgactgctc gaggaagggg
     2041 aaccgtttgc ggagagccgt gacgagcgtc gtttttcccg tgcccgacgg ggccgatatg
     2101 acgaaggccg agccgcgtcg cgcggccggt ccctgcgtgg tcgttttctt tctcatggtg
     2161 ccggtgcctt tggtgaacgg ggtactatat accaaacaaa aaagaaagtc agagaatttt
     2221 tttacgacgc aaggtgaggc aaagggcagg gtcaggaaca gcaccgcgcg cacggctcgt
     2281 ccggtccctc gagttcgagg gtgcaatggc cgacgccgag ttcttccatc gcggcggtcg
     2341 tctcccgttt gacgcggcag gccgtctccg gcgtcaccgg gccgtcgagc accagatgga
     2401 ccgtcagtat ggtccgttcg ccgtcaagac tccagagatg caggtcgtgg aacgaggcga
     2461 cccccgcgat cgccgtgagc cgcgcctcga tagccgcgag atcgacaccc tccgggatcc
     2521 cctgcagaag tatcttggcc gatgaccgga gcgctccgag cgccgttacg agtatgaagg
     2581 cggcgatgcc gagcgacagg agcgggtcta tccagtacca gccggtgaag tggatgacga
     2641 ccgccccgac gaggaccgcc gcccacccga gcacatcttc cagtaggtgg agcgccacca
     2701 ctttcgcgtt ctgcgaggtc ccttttttga ggcgcaggac cgccgcgccg ttgacgatga
     2761 tgccgagcac cgcgagcgcc aacatgcccc atgtgacggt cggttgcggc gtaaggagcc
     2821 gcggcacggc ggcgaatacg atgaccaccg acccgacgat caggatcacg ctcccggcga
     2881 gcgccccgag aagcgagaaa cgcttgtagc cgtaggtata acgccggtcg cgcccgcggt
     2941 ccgacacctt ctgcagatac cacgccagag cgagtgcgag gctgtcgccg aggtcgtgga
     3001 gcgcatcgga aagtatcgcg atgctgttgg tcagcaggcc gccgacgatc tctatcagtg
     3061 cgaacgagaa attgagaaaa aaagcgacgc cgatgtggcc ggtgtcgccg tggtcgtggc
     3121 catggctcat gccgccccgt cagacattga accggaaatt gatgatgtcg ccgtccttga
     3181 cgacatactc cttcccttcg agacggtagt ggcccgcttt cttgcattcg gcctctgagc
     3241 cgtacttgtt gaagtcgtcg aagtgtatcg tctcggcgcg gatgaagccg cgctggatgt
     3301 cgctgtgtat cttccccgcc gcctcgaccg ccggggtccc cttgcggatc ggccacgccc
     3361 gcacctcgtc ctcgcccgcc gtgaggaacg atatgagccc gagcgtttcg taggcggtcc
     3421 ggatgaaccg ggcgcgggcc ggttcggtga gcccgtagcc cttgaggaat tccgcctgct
     3481 cctccgcggc catctgggcg agatcctttt cgagcgtcgc gcagacggcg aacgacagca
     3541 cgccgtcggc cgcgatcgcc tcggtgagcc ccgccgggag cggcgcgccc ccctccggtt
     3601 cgttgacgag cgcgatccgc ttcttgagcg tcaggaaatt atagttggcc atgtgggcga
     3661 gttcgtcggc agtgaaggcg tcgggcgccg ggaaggtgct tttttcgagt atcgcgtgga
     3721 gtttctttat cgccggcatc tcgggcgggt tcttcttctc ttttgcctcg cgctctatcc
     3781 gcttctcggc ggtgatgagg tcggtgagca ggagttcgtc cttgagtttc cggtattcgg
     3841 cgagcgggtc tttcggctcg gcggtcatca ccgaatcgaa attgcgcagg acgaagatga
     3901 gcagatcggc cttttggatg agattcttga cctgcgacgg aaggagcgcg tccttggtcg
     3961 ccgcgatgtt gaaatcggtc aggacgaatt cggcgtaggt cttcttcttc ggtttgtata
     4021 tctcgttgag ccggtctatc cgcgtgtcgg ggacctttat cgtcccgagg ttctcctcct
     4081 tcttcgcctc cgcgatgccg gtaagcgcct cgaagacggt cgttttgccc gaaacgggaa
     4141 gcccgataag tgccgcgcgc atggtatctc cttctgtttt aagttcccgg ggtcgctttc
     4201 ttttcgaaca gttcgatgat cgccggcagg ttcatccgtt tggcgagcag gagcggggtg
     4261 tcgccgttgt tgtcggcgac atcggtccgc gcaccttttt caagaagcat cttgacggtg
     4321 gtcgcgttgc cggaggggat gacatagaag agcgcggtct tccccgaggt ggtctcgcgg
     4381 gcgttcacat cggcccctgc ttcggtgagc aacgccacga tagggtggaa cccgtaggag
     4441 gcggcgacaa gaagcgccgt ccagccgccc gacacgagtt tctgttcgag cgtcgccttt
     4501 ttctcgatga gcagtttcac gagcgcctga tcgcccgcct gcgccgcaaa gtagaggatg
     4561 cttgctcccg aagagtcggt gaagttgaca tcggccccct tcttgatgag gaattcggcc
     4621 atcggcgttt tacggtaagt gaccgcctcc ttgagcggcg tatacccgtc gctcgactgg
     4681 aagttgacat cggccccttt tgagacgagg tattcggcga tcgggagaaa gccgagccgc
     4741 gcggcgagtt ggagcggcga gtagccgttc gggtccatct cgtcggcggg gcgccccgtc
     4801 tcgagaagtt ccttcacctt cttgagatcg ttcttttgca ccggataggc gaccgaatcc
     4861 ttgtccaccg cgccgatgtc gttgtagggt tcctgatagg ggtatttgtc tcccgcaaag
     4921 cccagtttct ccgccttgtt gaagccatcc tcgaacccct tcgcataggc ctcggcatag
     4981 gtcttcgcgt agtcggcggt caccggcacg gtctggcacg aaacgagagc gaaaaagccc
     5041 gtcaacaaac cgataatgat cgatcttttc atggttccct ccctgcggat atggtcgctg
     5101 atcacaccag tttttccttg accagttgac ttgccagttt gccgtcgtaa cggccggcgt
     5161 agagttcctt gagtttcgcc atcacctgcc ccattgcctt gggcgacttc tcgggaaggg
     5221 tcgcgacgat atcgtcaatg actttagcga gttcttcggc ggtcaactgc ttgggcaggt
     5281 atccttcgag tatcgtccgc tccgcctttt ccttgtcgca cgcctttcca tcgccggtca
     5341 ggatggcgat cgtctcgtcg atgttcttca tgaatttgcg gatgatctgc atcacctctt
     5401 cgggggtcgt ttcgcggttg ccagcgttct tgccgaccgc catcgcctcg ccgaggagcg
     5461 tcgagaggag attcgacctc accagatcgt gagttttttt tgcctcgaac aggtcttttc
     5521 tgatgttcgc gagcatcggt aactccctta tcggttatga atttatgcca agtttttcgc
     5581 gccacttttc tatcgccgca tcctcgatct tggggtagag gacggccggt tcgccgatgg
     5641 tatgtcccgg tttcatcggc tcactgcaga gggcgttgag atcgaaggtc tcgggaaggt
     5701 tcagcatctt cttcaccttg cgcgacgccg ccggggtgat cggctcaaag gcggtcgcga
     5761 gggtgtcgat gaggttgagg caggtgagga gcaccgcctt gcaccgtttc tcgtcggtct
     5821 tccggagtga ccacggcgcg ctcgtatcga cgtatttatt agcgatgcgg cccagttcca
     5881 tcaggtggaa gaccgcgtcg cggaccttga aggtatcgta gcattcctta atgatatcgg
     5941 actctttctt cgcctcagcc cacagtgcct tctctgcatc ggtaaaggtg acatcgccgc
     6001 cgagggtccc gtcgaagaac ttgcgggtga atgagaggat acggttgacg aagttaccgt
     6061 agatgtcggc gagttcggag ttgttggcgg tctggaaacc cttgagcgag aaatcggcgt
     6121 ccttcgcctc gggagcgatt tgcgcaaggt agtaacggat cacatcgctg ttgaaatcgt
     6181 tcaggaattc atgcagccat atcgcgtagt tcttcgaggt ggagaacttg tctccctcga
     6241 ggttgaggaa ctcgttggcc ggtatctcgt cgggcagttt ccagtcgcct ttctgtccca
     6301 tcagcatcgc gggccagatg atggcgtgga acacgatgtt gtccttgccg atgaaatgga
     6361 ccaacttaac atcctttccg ccctgccacc atttcttcca atcctcggtg cgaccgaggt
     6421 tccggaacag ttcgatggta aaggagatgt agccgatggg ggcgtcgaac cagacataga
     6481 gcactttccc tttcgccccc tcgatgggca ccggtacccc ccacgagata tcgcgggtga
     6541 tgccgcgcgg ttcaagcccg cgctggaacc actgctcgca gaaggccttg acattggcct
     6601 tccagtgtgg tttccctttg agccagatat cgagatcttt ctggaactta tcaagatcga
     6661 ggaaccagtg gcgcgaactt ttcaccaccg gcgtctttgc gcacagtttg catttgggct
     6721 ctttcagttc aagcgagttg agggcgttgc cgcagtagtc gcactggtcg ccgcgcgccc
     6781 ctttgccgca atgggggcag accccttcga catagcggtc gggaaggaac atcgcatcgt
     6841 ggtcgcagta gaactggtcg acatcgcgct ccgagatgaa accgttcttc cggagcgtca
     6901 ggaagaaatc ctgcgacagt ttcgcgtggt cggggcaggt gctcgtcccc gagaagatgt
     6961 caaaactcat ctcaaggcgg cggaatgcgt cctctattat cctgtgattc tcggccacca
     7021 cttcgggcgg tgttttgccg ttctgttcgg cgttgatggt gatcgggacg ccgtgttcat
     7081 cggtgccgca gatgtagatg acctcgcggc cgctgttgcg caggaaacgg acgtagatgt
     7141 cggcgggaag ataggcgccg gcgatgtggc cgaggtgtat cgggccgttc gcgtacggga
     7201 gcgccgaggt gacgagatac cgcatgagtt cctccggttg acaaaaacgg ggggagtata
     7261 gtcaaaaaca ggggcgcgtc aaatcgagga aagaggtcat acctcgctat accgccagat
     7321 cgctgtcggc gggctcttcg tgtttgcgta cgaaaagcgc catgccgacc gtcagcggcc
     7381 cgatgcgccc gccgatcatg agaagcacga cgatgaattt ccccagatcg gtaagcgttc
     7441 cggtgacccc catgctgagc cccaccgtcc cgatggccga cgccgcctcg aagattatct
     7501 tgtcgaacgg tgccgtttcg gtgagatcga gcagatagac gccgaccgtc agcagtatga
     7561 gatagagcgt ggtattggcg atcgcctgcc atatccgttc gaaagggatg accttcccgc
     7621 tgaaagatgc tttttgttcg ccgcgcaggg cgctgcggac cacgccgagt atcgccgtga
     7681 aggaggtgct cttgacgccg ccgccggtcc ccgacggcga ggcgccgatg atcatcagga
     7741 gcgtgaggac cagcaccgaa cctttcgaaa gcgcgccgag atcgatggtg ttgaaaccga
     7801 cggtggtcat cgccgtcatg cactggaaaa aggcggcgag caaccggtcg accggagggc
     7861 gggccgttat cgtcggttcg gcgaggaaca gcagagaggt accgatgatc gtcatccaga
     7921 cggtcgctcc gaggatgatg cggctggtaa gcgtcacctg cgtggtctcg ccccggaacc
     7981 ggcgccagta atcgatgaac acgatgaacc cgatggcgcc gagataactc aggagagcga
     8041 tgatcccgtt tatccagaag ttgccggaga aagccgaaaa actgtcgttg aaggtgctga
     8101 atccggcggt acagaaggcc gaaatactgt gaaaaaaagc agtgtagaac ggacgcggtg
     8161 tcccggccgc cgtgaaagcc cagcacaaaa ggagcgttcc gaccgactcg atgacgacgg
     8221 tgaacagaac gacgcctctg atgaagcgga acacgtcgaa cgaccgcggc agagaaaaga
     8281 cggcgtgcgt cacctcggta cgcgccgggg gcagatcggg acggcgcgac agaatgatga
     8341 acgacccgaa ggtcatgaac ccgacgccgc atatctggat aagggcgagt atcaccaact
     8401 gaccgaaaag gctgtaattg tcggagacgc tgatggagac cagcccggtg gtcgacatcg
     8461 ccgaggcggc gatgaagagg ttgtcgatcg gggcgatgtg atcgacggtg cgggcgatgg
     8521 gaagcgagag aagcgcccaa ccgacgagaa catacgaaag atagccgagg aagacggcgc
     8581 gcgccgggtc gacccggccg gtgaagaata gaaatcgtcg caggaggcgc gagaggatca
     8641 ggcgacctct cttgaaaaga cgaaggcgga ccgcagacat gacacagtac ctcacaaaaa
     8701 atttgtcaac gagaggaagc cggccggcgt ctgtcggggg gtttcctctt cccacgctta
     8761 cgaggttagc tgacgggctc gggcggaaga gtgccctacg ccataacggc gatgcgcccc
     8821 gggaaaattg ggtcccccgt tcccgccggt ggcgggatta agcggttacc cttgtacgct
     8881 tttcagaaaa aaagtcaaga ggttatttct cggcccggat cactgttcct tcaggaacgc
     8941 ctcgagcatc tgtatcagcg tggcgggcgg caggaccgcg ccggcggcgt cggggacatc
     9001 gatgagattc tccgccttga ccatgcgccg ctcctgcacc gagaggtcgg ggttgagcac
     9061 gaacaccggc ccttcggcgg tgaaattctt gaaccggccg caagagacga cgagttcgtt
     9121 cgacgatatc gtgcggagca cgctgccgca ggggtagacc cccgcgatcg tcacgaaggt
     9181 atcgacgatg tcgcggcgga acgcccccgc cccggcgttc ttcagcatgg agcggagcgc
     9241 ctcgggtcgc gaaaggagcg ggccgtcgtg ccaccgccgg gtcacccggt cgtagtaggc
     9301 ggcgaccttg agtatctcgc tggggacggt gccgcaggcg gtcccctcca tcatgagccc
     9361 cacctccgaa agatcggtga gccgcgcgga ggcgttcaac gccgcctcta tccgggtgta
     9421 actgaactcg tccatgcgtg aaaggaccgt gtagcccgtg tcgggatcgt cggaaagata
     9481 ctggaaatag gcggcgatgg cggtccgttt gacctcggac ggttcaagcc cgagccgcag
     9541 ggcgatgagg atggaaaaga tggtgcgcgc ggcggcggcg gcccccgcga cgcttccccg
     9601 gtaggcgggg agggcggcca gcagcatcag gagcgcgtag ttgcccgggt cggaaaggtt
     9661 ctgcgctatc gatatgaggt cctgcacgct tcgttcaacc gtccggagcg gtatgttctc
     9721 cgccgccttc accatcttcg cgtaatcggg gtactcggcg gccagtttcc ggtgcgcccg
     9781 ccggatcgcc tgcggaacgg tgtatatcgg cagattgcct gactcctcgg ggtcgtaggg
     9841 gaacacctcg atatgggtga gcggcggcag gtcggcccgt acccgttcgt aggcctgtat
     9901 cttgggctcg cgaccgaccg gatatttagc gagtaccgcg aagaaaccga gtatctgtct
     9961 gtcatccacc cctttcttga acacgaaccc ggagaggcac agcgagagga acaggtcgta
    10021 gatgtcgtcg aaatatttgt catcctgccg ttggccgcgg atgcgtacct tgtcgacctt
    10081 cacatcgatg ccgtcgaata gtatcgagaa ttcatccttc tcggcgaaaa agaagtccag
    10141 tgtctttttg aaataggcga tctcctcgtc gatacgctgg ttctcgacac cgtacatctt
    10201 gacattcttc cagaaagtga agagcgactt ggtgaaattg taccgccatt tcttcgggga
    10261 attctcgaac tcgggcagat tgcgtcgttc ttccatcggt cacctaccgt cccgccagta
    10321 tctctttggt ctcggcatcg ccgtcctttt tcgccttttt ttacggcttc ggacttgttg
    10381 ataatcgtct c
//
LOCUS       DAOQUE010000033        10367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_7214_length_10367_cov_4.076707, whole genome shotgun sequence.
ACCESSION   DAOQUE010000033 DAOQUE010000000
VERSION     DAOQUE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7214_length_10367_cov_4.076707"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4089)
                     /locus_tag="PKH10_01920"
     CDS             complement(<1..4089)
                     /locus_tag="PKH10_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26912.1"
                     /translation="MNMKKLFKNLAVLFAMLVVAACTTERSAVDKTEELALDKKLFDG
                     EFFYRQTVTDLPYTADFAFIGESNDGKIIRWKITKNWLIAYNVHDKIDIVDTAEQVAT
                     NETPILAYPIAMHFDILPQENPTTGEDMPMLVPNTDKPWNERRYFLIDHSGSAVENAE
                     LKYLWMTQEQDNPFYFSAVEAATDMEFYAHDGSYIPPKKYADMLAAEDDPFNKEVEWF
                     QFRSERYLVFFNNWNSIYSWADIEEFINTEPARMVARHTFKKINRDVVGRRHYDASTQ
                     QFTWKKGPKDNGFRPLEFPDELFREFGYFTNLFKGYDPESGYKEKDYHELANYFNIAW
                     AKAGEGEGVAQDWSFTCAPNQATATTYNACLANLKYQQKMVFTSSAETPKRMLPANCA
                     ITKDYNHAMLAARFAAMNPGSDTREFEKWYVANYKNDFFKDVKMGSKTVKVRDMEWYL
                     KPPAGWESKCFYSNDDAFIQRWNGDFVEDKDINEIVVFVKNPVEAYIPARDGKHLADA
                     EGYDAFETENNENAFFICTTADEAQNADEGKITHFENCYTNEEEMTALYTDEFGRSYW
                     KGDRYDCKVLKKGETCPAGYEPVKAACEINDSRTCRFYDNGLGPPKLRHRYMNGGLDV
                     ALINWVDQPTEYGILGVSQWNVNPETGQTLGAGSNIAGSVLQWVMNRTVELARILIDE
                     DDPAAWDWEQLIAPEEYSERPSVVFQNSPAQMKPRFVKNSTAMKKKDLLDPLLKAEQQ
                     TEKVHKLMEKISEKQHNYKRFDYNNLKGTKWEREMVPYSMRTAMFPWSIDDDQDSWVY
                     SDGEMNVMRPFVSGPEAFNIEMLRYLEGMKMDFFMDETFLDGAIIQYLKQKMTALRKK
                     YPNYKDEDKEKYVAEFTQTIFDELEPLTYKGVAEHEMGHSVGLRHNFSSSADQLNYHD
                     EYFATGNYPAMLKEMKEKAQELNDEGRDPATWGREIYNVKRSFESKMNYYTYVSIMDY
                     QREAYIHAIGLGKYDKAAIKFAYGRSVEQYRTDDEGFVEMDETNPLRDAAYPFPVLIN
                     RSFVDRESGKLEEIADFPRETITYEDPSEGKVMRVVAALKHDMLGRLIIGEDGNNYKT
                     NETKYLVNDGSIHKYIFFSDEKQTDEPINKVFDAGYTGADSVRFMADTEQSYYFTRFF
                     RRGNPKFREFRGRTRYKMILDTLLDKYKYVHYLLDFNYNVAKAGWFGNLPIWTSGNGC
                     GTLQYSLSTDIAEPKITLDNAQCIEYLKGMQGIEYWLEGDELKTLTPFGMGDYIVAGM
                     VGVNHFLHNVLYRPDVTEYLRMGTSFDADINSKIETSLGQEAVWVSNPYILDGETIAG
                     GSTGLPDPIVPDPVVGRYHRDHYWYQDDPSVYYDKVLWRGYSE"
     gene            complement(4103..5446)
                     /locus_tag="PKH10_01925"
     CDS             complement(4103..5446)
                     /locus_tag="PKH10_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26913.1"
                     /translation="MANLWKCLWLVAFLAVMPLGAQEPEEGAAEEAGGDDPAAAQEEA
                     KADEKKEEEKATEGATAEQPAPAAPVAEEESAPAAPAAEAQPAPVAPAAEAQPAPAAP
                     VAEAQPAPIAETTPAGTPGSEEVTAEAEVKEEEKKEEEGKEEEKEAKKYSVGLNNALS
                     QNINKDRPNFGYALTVTGSYTIPGDINLGASIGLAYNLAYLRDRTTSDAGDYQTSSLN
                     HYSFDGTPLGIDISRVFTIPTVEIGVTPAINWSIPFTNRMMWEVYTLRSTLTPSVTID
                     RAFKLMDDMTLSVAYTCAYSYNFAEEDAVIGKNTNEVFDINVQMSLNNMLTVSWAYKG
                     FSIRVGAGYDSSILYGELKKSADTQSFNQLWNHTVIFNGGVGYSMAGFTDESDRFSFG
                     LGFTTAGPEYENGNYGGFSYNAGGKEGRTGINGFTVPFAGKFTKITATIGYNYSF"
     gene            complement(5652..7061)
                     /locus_tag="PKH10_01930"
     CDS             complement(5652..7061)
                     /locus_tag="PKH10_01930"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT26914.1"
                     /translation="MYLLLYRDNRHLFSFYINSAKKKVVIGGDEGDILFPPDMRGYEL
                     TLRREFDFRSRQYAWVLYPSSKGFTLNGKEARPGDHFSDSDVVAIRDFSFSFSQFSHL
                     ATAGEGGDTAKGRTVVVDTERTAYKSILVEHDGRSKNFLFRDGREIVVGRKKTDISLL
                     QPDVSAEHLVISMKEAGIFFWNKGKNGTYINGMRVENGELQEGKYRFLIAGKHPVTVT
                     VLRERVAESFHKGSLEPLFEKVEGWFQRPELFGGRPIILFSGESGVGKEVFAEFAHAM
                     SGRKGEFVTYNAAAIPETLAESELFGTCKGSFTGAEDRAGAFLQADGGTLFLDEIAEM
                     PLSLQGKLLRVLEDWTVRRVGESSGGKKVDLLMILATNRDLAKEIAAGRFRKDLFYRI
                     STLAVTIPPLRDRRADILPLARHLLFSLTGRDASFTPEAVTALESHDWPGNVRELKSV
                     MTRFAYTGKDPFEVSDVSV"
     gene            complement(7140..8825)
                     /gene="ftcD"
                     /locus_tag="PKH10_01935"
     CDS             complement(7140..8825)
                     /gene="ftcD"
                     /locus_tag="PKH10_01935"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNT26915.1"
                     /translation="MARIIECVPNFSEGRDRVVIDQITAEICKVNGAKLLDVDPGWDT
                     NRTVVTMVGEPEPVLEAAFNAVKKAHELIDMRKHKGAHPRFGACDVCPIIPVSGITME
                     ECVALAHRLGKRLGDELDFPVYFYEYAATTPERKNLAKVREGEYEALEARLKDPRWKP
                     DAGPAEFRPAKGATAVSAREFLVAYNVNLNTVDKKKANDLALRIREQGYPKRDDKGNL
                     VKDEKGEKIMMPGKFKNCKAIGWYVDDYKRAQISINLTNYKVTSMHHVFDELSERARE
                     AGLRVTGSELVGVVPKQALIEAGIHYLEKQGGCAGQSDAELIKIAIQSLGLSDVAPFR
                     PEEKVIEYLLHEKKGLVDLSVTGFCDLLASDAPAPGGGSVAALCGAMAASLGAMVANL
                     THGKKGYVKHDDLMNDTAKEAQKRKAEFLSLIDEDTDAFNRMMAAMSAKKKAEKDGAD
                     VERERAAIEETEALKNATLVPLTCLEKSGALCPLLATVAEKGNANAVSDAGVGAHLVK
                     AAAHAAALNVRINLNNFNDMDNFKSETLKKMNALLAEAERGADAIIAVVDKKL"
     gene            complement(8950..9879)
                     /locus_tag="PKH10_01940"
     CDS             complement(8950..9879)
                     /locus_tag="PKH10_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26916.1"
                     /translation="MKRVVLAAVAALFLTVACANSEQSVVLEKFIPLTEQNSCKLEIG
                     SNLWYDIGYMDIYFTQQYLLPFKMVNYMNVSSQTGDMNPEQPYDIHEANRWRLERLIL
                     EYELPPVPGVDGGAWATRTVESGVVIDPEGGKIAGAVDLFTTAQHTNLRNIFTTLFNA
                     GVIFDWETYPIIVDVQGEGIKLGGGDRIRTNKLRFSLVPLYGLSVMMGSVYPVNDDWP
                     TDEELQEQYPVEAAYEQAKYERDQEIYDVIMEYCYFPSSHLEGCFPGQDGALLDCYTF
                     GDADDLIRSLFPGYECCPIEQPQEPEEPETATP"
     gene            complement(9908..>10367)
                     /locus_tag="PKH10_01945"
     CDS             complement(9908..>10367)
                     /locus_tag="PKH10_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26917.1"
                     /translation="SEAMIQQGISVPITTRSTGGEVTTKFVDATLILKSTPHITSDGS
                     ILMKLNINKSEADYSNANYLGESAIMKKEAQTEVLVRSGDTIVIGGVYTNKVSKTVRK
                     VPVLGDIPILGWLFKRIDERVERSELLIFITPRIMNKVRSAMPLTRSEEE"
BASE COUNT         1993 a         2983 c         2951 g         2440 t
ORIGIN      
        1 ctcgctgtac ccgcgccaga ggaccttgtc gtagtagacc gacggatcgt cctgatacca
       61 gtagtgatcg cgatggtagc ggcccacgac cggatcgggc actatcggat caggcagacc
      121 ggtgctgccg ccggcgatcg tttcgccatc caggatgtac gggttggaca cccagaccgc
      181 ttcctgaccg agcgaggtct ctatcttgct gttgatgtca gcgtcaaaac tggtacccat
      241 cctgaggtat tcggtgacat cggggcgata gaggacattg tggaggaaat ggttgacgcc
      301 gaccatgccg gcgacgatgt agtcacccat gccgaacggg gtcagggtct tcagttcatc
      361 gccctccaac cagtactcga tgccctgcat gcctttcagg tactcgatac actgagcgtt
      421 atcgagcgtt atcttcggct ccgcgatatc ggtcgagaga ctgtactgca gggtaccgca
      481 cccgttgccg gaggtccaga tgggcaggtt gccgaaccaa ccggccttcg cgacattgta
      541 gttgaaatcg agcaggtagt ggacatattt gtacttgtcg agcagggtgt caagtatcat
      601 cttgtaccgg gtgcgaccgc ggaattcgcg gaacttcggg ttgccgcggc ggaagaaacg
      661 cgtgaagtag taggactgct cggtgtcggc catgaaacgg accgaatcgg cgcccgtgta
      721 gccggcgtcg aacaccttgt tgatcggctc gtcggtctgt ttttcgtcgg agaagaagat
      781 atacttgtgg atggagccgt cgttcacgag atacttggtc tcattcgtct tgtagttgtt
      841 cccgtcctcg ccgattatga gtcgaccgag catgtcgtgc ttgagcgccg cgacgacgcg
      901 catgacctta ccctccgagg ggtcttcata ggtgatggtt tcccgcggga aatcggcgat
      961 ctcttcgagt tttccgctct ctctatcgac gaacgaccgg ttgatgagaa ccgggaaggg
     1021 ataggccgcg tcgcgaagcg gattggtctc gtccatctcg acgaaaccct cgtcatcggt
     1081 ccggtactgc tcgaccgaac ggccataagc gaacttgatc gccgccttgt cgtacttacc
     1141 gagcccgatc gcgtggatat aggcctcgcg ctggtagtcc atgatggaga cataggtgta
     1201 gtagttcatc ttcgattcga aggagcgctt gacattgtag atctcgcggc cccaggtcgc
     1261 cggatcgcgc ccttcatcgt tgagttcctg cgctttctcc ttcatctctt taagcatcgc
     1321 cggatagttt ccggtcgcga agtactcgtc atggtagttc aactgatcgg ccgatgaact
     1381 gaaattgtga cgcaggccga ccgagtgccc catctcatgc tccgcaacgc ctttgtaggt
     1441 cagcggctcg agttcgtcga agatcgtctg ggtgaactcg gccacatact tttccttgtc
     1501 ttcgtccttg tagttggggt actttttccg gagcgcggtc attttctgct tgaggtactg
     1561 gatgatggcg ccgtcgagga aggtctcgtc catgaagaag tccatcttca tcccttcaag
     1621 gtagcgaagc atctcgatat tgaacgcctc gggacccgac acgaagggac gcatgacatt
     1681 catctcgccg tcggagtaga cccacgaatc ctgatcgtcg tctatcgacc acgggaacat
     1741 ggcggtccgc atgctgtagg gaaccatttc gcgttcccac ttggtcccct tgaggttgtt
     1801 gtaatcgaac cgcttatagt tgtgctgttt ctccgatatc ttttccatga gtttgtggac
     1861 cttctcggtc tgctgttcgg ccttgaggag cggatcgagc aggtctttct tcttcatcgc
     1921 cgtgctgttc ttcacgaagc gcggcttcat ctgggcggga ctattctgga atacgacgga
     1981 cggacgctcc gaatactctt cgggcgcgat gagttgttcc caatcccacg ccgccgggtc
     2041 atcctcgtcg ataaggatgc gggcgagttc gacggtgcgg ttcatgaccc actggaggac
     2101 cgaaccggcg atgttggatc cggcgccgag cgtctggccc gtctcgggat tcacattcca
     2161 ctgggaaacg ccgagtatgc cgtattcggt cggctggtcg acccagttga tgagggcgac
     2221 atcgagaccg ccattcatgt agcggtgacg gagtttcggc ggacccagac cattgtcgta
     2281 aaaacggcag gtgcggctgt cgttgatctc gcaggccgcc ttgaccggtt cataaccggc
     2341 gggacaggtc tcgcccttct tgagcacttt gcagtcgtag cggtcgcctt tccagtaact
     2401 gcgaccgaac tcgtcggtgt aaagcgccgt catttcctct tcgttggtgt agcagttctc
     2461 gaagtgggtt atcttcccct cgtcggcatt ctgcgcctcg tcggcggtgg tacagatgaa
     2521 gaaggcgttc tcgttgttct cggtctcgaa ggcatcgtac ccctcagcgt cggcgagatg
     2581 tttgccgtcg cgggccggga tataggcctc gaccgggttc ttgacgaaca cgacgatctc
     2641 gttaatgtcc ttgtcctcta cgaagtcgcc attccacctc tgaatgaagg cgtcgtcatt
     2701 ggagtagaaa catttcgact cccatccggc gggcggtttc aggtaccatt ccatgtcgcg
     2761 taccttgacc gtcttggagc ccattttgac atctttgaag aaatcgttct tgtagttcgc
     2821 gacataccat ttctcgaatt cacgggtgtc gctcccggga ttcatggccg cgaaacgggc
     2881 cgcaagcatg gcgtggttgt aatccttggt gatggcgcag ttcgccggga gcatgcgttt
     2941 cggtgtctcc gcggaggagg tgaacaccat cttctgctga tacttgaggt tggcgaggca
     3001 agcgttgtag gtcgtcgcgg tcgcctgatt gggggcgcaa gtgaagctcc aatcctgcgc
     3061 gacgccttcg ccttcgcccg ccttagccca cgcgatattg aaatagttag ccagttcgtg
     3121 ataatccttc tctttgtagc ccgactcggg atcgtagcct ttgaagaggt tggtgaagta
     3181 accgaactcc cggaacagtt catcggggaa ctcgagcgga cggaatccgt tgtccttggg
     3241 ccccttcttc caggtgaact gctgggtcga cgcatcgtag tgacggcgac cgacgacatc
     3301 gcggtttatc ttcttgaagg tgtgccgggc gaccatacgg gccggctcgg tgttgatgaa
     3361 ctcctcgata tcggcccaac tgtagatgct gttccagttg ttgaagaaca ccagataccg
     3421 ctcggagcgg aactggaacc actcgacctc tttgttgaag ggatcatctt cggccgcgag
     3481 catgtcggca tacttcttcg gcgggatgta agaaccgtcg tgggcgtaga actccatgtc
     3541 ggtcgccgcc tcgacagcgc tgaaatagaa gggattgtcc tgttcctgcg tcatccacag
     3601 gtacttgagt tcggcgttct cgacggcact ccccgaatgg tcgatgagga aatagcgccg
     3661 ttcgttccac ggcttgtcgg tgttcgggac gagcatcggc atatcctcgc cggtcgtcgg
     3721 gttctcctgc ggcaggatgt cgaagtgcat ggcgatggga taggcaagga tcggcgtctc
     3781 gttggtcgcc acctgttcgg cggtgtcgac gatgtcgatc ttgtcatgca cattgtaggc
     3841 gatgagccag ttcttggtga tcttccagcg gatgatctta ccatcgttcg actcgccgat
     3901 gaaggcgaaa tcggccgtgt agggcagatc ggtgaccgtc tggcgataga agaactcgcc
     3961 atcgaacagt ttcttgtcca gcgccagttc ctcggtctta tcgaccgccg agcgctcggt
     4021 ggtacaggcc gcgacgacca gcatcgcgaa aagcaccgcc aggttcttga aaagtttttt
     4081 catgttcata ccaacctcca tgttagaaac tataattgta gccgatggta gccgttatct
     4141 tggtgaactt gcccgcaaag gggaccgtga acccgttgat gcccgtgcga ccttccttgc
     4201 cgccggcgtt gtaactgaac ccgccgtagt tgccgttctc gtactcggga ccggcggtgg
     4261 tgaaaccgag cccgaagctg aagcggtcgc tctcatcggt gaagccggcc atgctgtagc
     4321 cgacgccgcc gttgaagatg accgtatggt tccacagctg gttgaacgat tgggtgtcgg
     4381 cgctcttctt cagttcgccg taaagaatgg agctgtcgta accggcccct acgcgtatcg
     4441 aaaagccctt gtaggcccac gaaacggtca gcatattgtt gagcgacatc tgcacattga
     4501 tgtcgaacac ttcgttggtg ttcttaccta tgaccgcgtc ctcctcggcg aaattgtagg
     4561 agtaggcgca ggtataggcg accgagaggg tcatgtcatc catgagtttg aacgcacggt
     4621 cgatggtcac cgaaggggtg agggtcgaac gaagcgtgta gacctcccac atcatccggt
     4681 tggtgaacgg tatagaccag ttgatcgcgg gggtcacgcc gatctccacg gtcggtatcg
     4741 tgaagacgcg gctgatatcg atacccagcg gcgtaccgtc gaaactgtag tggttgagac
     4801 tcgaggtctg gtagtcaccg gcatcgctgg tggtccgatc ccgcagatag gcaagattgt
     4861 aggccaaccc gatggaggcg ccgaggttga tgtcacccgg aatggtgtag gagccggtca
     4921 ccgtcagcgc ataaccgaaa ttcggtcggt ccttgttgat gttctgcgaa agcgcattgt
     4981 tgaggccgac gctgtacttt ttggcctctt tttcctcttc cttcccctct tcctcttttt
     5041 tctcctcttc cttcacctcg gcctcggcgg tcacctcttc ggatccgggc gttcccgcag
     5101 gcgtcgtctc ggcgatcgga gcaggctgcg cttcggcgac gggcgcggcg ggagccggct
     5161 gcgcttcggc ggcaggcgcg acaggggccg gctgcgcttc ggcggcaggt gcggcgggag
     5221 ccgattcctc ttcagcgacc ggcgcggcgg gagccggctg ttcggccgtc gccccttccg
     5281 ttgccttctc ctcttctttt ttctcgtcgg ctttcgcctc ttcctgcgcg gcggcgggat
     5341 cgtcaccgcc cgcctcctcg gcggcaccct cttcgggttc ttgcgcaccg agcggcatca
     5401 ccgccaagaa cgcgacgagc cacagacatt tccacagatt cgccacaacg acctcctata
     5461 tcagcttttt gctatttctg ttatcgtgtt gtccgagaac atgggtccct ttccgaaatc
     5521 ggtccacgct agtttctcct tcctcatttg tcaattttat taatgaacca aaaaaacaca
     5581 cacctgttca gcaaccaccc ttccggactt ttttcgcaag tcatttcgga caaatggtgt
     5641 cttattgggg gctacacgga aacatccgag acttcaaagg gatccttgcc ggtatacgcg
     5701 aaccgggtca tgaccgactt aagttcgcga acattgccgg gccaatcgtg cgactcgagc
     5761 gccgttaccg cctccggtgt gaaggacgca tcccgcccgg tcagcgagaa cagcaggtgt
     5821 cgcgcaagcg gcaggatatc ggcgcgacga tcgcggagcg ggggtatcgt gaccgcgagg
     5881 gtcgatatgc ggtaaaagag gtccttgcgg aaacgacctg ccgcgatctc tttcgcaaga
     5941 tcgcgattgg tcgcaagtat catcaggaga tcgaccttct tcccgccgga actttcgccg
     6001 acgcgccgta ccgtccaatc ttcaagcacc cggagcagtt tcccctgcaa cgacagcggc
     6061 atctcggcta tctcatcgag gaagagcgtc ccgccgtcgg cctgcaggaa cgcaccggcc
     6121 cgatcctcgg cgccggtgaa actccccttg caggtaccga acagttcgct ttccgcaagg
     6181 gtctcgggta tcgccgcggc gttgtaggtg acgaattccc ccttacgacc gctcatggcg
     6241 tgggcgaact cggcgaagac ctccttcccc accccgctct cgccgctgaa caggatgatc
     6301 gggcgaccgc cgaagagttc cggtcgctgg aaccacccct ccaccttctc gaacagcggc
     6361 tccagggatc ccttgtggaa cgactcggcg acccgttcac gcagaacggt gacggtcacg
     6421 ggatgtttgc ccgctatcag gaaacggtat ttaccctcct gtaactcgcc gttctcgacg
     6481 cgcatcccgt tgatgtaggt gccgttctta cccttgttcc agaagaatat ccctgcctct
     6541 ttcatcgata tcaccagatg ttcggcgctg acatcgggct gcagcaggga gatatcggtc
     6601 ttcttgcgcc ccaccacgat ctcgcgacca tcgcggaaca gaaagttctt cgatcgcccg
     6661 tcatgttcga caaggatgct tttataggcg gtcctctcgg tatcgaccac gaccgtgcgc
     6721 cccttcgcgg tatcgccccc ctcgcctgcg gtcgcaagat gcgagaactg ggagaaagag
     6781 aaactgaaat cacggatggc gaccacatcg ctgtcggaaa aatggtcgcc gggacgcgcc
     6841 tccttcccgt tcagcgtaaa gcccttggag gagggataga gcacccacgc gtactgacgc
     6901 gacctgaaat cgaactcgcg ccgcagggtc agttcatagc cacgcatatc gggcgggaac
     6961 aggatgtcgc cctcgtcgcc gccgatgacc acctttttct tggcactgtt gatatagaat
     7021 gaaaaaagat gccggttatc gcggtataag agcagataca tcgttcactt ccgtccacac
     7081 tcttgacggc atcttaataa agcggggcat gaaagcaatt ttttgaccga ccgggccggt
     7141 cagagttttt tgtcgaccac cgcgatgatg gcgtcggcac cccgttcggc ctcggcaagg
     7201 agtgcgttca tctttttaag cgtttctgat ttaaaattat ccatatcgtt gaaattatta
     7261 agatttattc gtacattgag cgccgccgcg tgggccgccg ccttgacgag atgcgcgccg
     7321 acaccggcat cagaaacggc gttcgcgttc cctttttctg cgaccgtcgc gagaagcgga
     7381 cagagagcgc cgcttttctc cagacaggtg agcggcacga gcgtcgcgtt cttcagcgcc
     7441 tcggtttcct ctatcgcggc cctctccctt tcgacatcag caccgtcctt ttcggccttc
     7501 ttttttgcgc tcatcgccgc catcatccgg ttgaaggcat cggtatcctc gtcaataagc
     7561 gaaaggaact ctgctttgcg cttctgcgcc tcttttgcgg tgtcgttcat caggtcatca
     7621 tgcttcacat aacctttttt accgtgggtg aggttcgcga ccatcgcgcc gagcgaggcc
     7681 gccatcgccc cgcagagcgc cgcgacactg ccgccaccgg gagccggggc gtcgctcgcg
     7741 agcagatcgc aaaaaccggt caccgagaga tcaacgagtc ccttcttctc atgtaacagg
     7801 tattctatca ccttctcctc gggacggaac ggggcgacat cggaaagacc gagcgactgg
     7861 atggcgatct tgatcagttc tgcatccgac tgaccggcac agccgccttg tttctcgagg
     7921 tagtggatcc cagcctcgat aagcgcctgt ttgggaacga cccccaccag ttcgctaccg
     7981 gtgacccgaa gaccggcttc gcgggcacgc tcggagagtt catcgaagac atggtgcatc
     8041 gaggtgacct tgtaattggt caggttgatc gatatctgcg cccgcttgta atcatcgaca
     8101 taccagccga tcgctttaca gttcttgaac ttaccgggca tcatgatctt ttcgcctttc
     8161 tcgtccttga caagattgcc cttgtcgtcg cgtttcggat agccctgttc gcggatgcgg
     8221 agcgcaaggt cgttcgcctt tttcttatcc acggtattaa ggttcacatt gtaggcgacg
     8281 aggaactccc gggcgctgac ggcggtcgcc cctttggccg gacggaattc cgcgggaccg
     8341 gcgtccggct tccagcgggg gtccttcaat cgcgcctcga gtgcctcgta ttcgccttca
     8401 cgcaccttgg cgaggttctt tcgttcgggc gtcgtcgcgg cgtactcgta gaagtagacc
     8461 gggaagtcga gttcatcgcc gagccgcttg cccagacggt gggccagcgc gacacattcc
     8521 tccatcgtga taccgctcac cgggatgatc gggcacacat cgcaggcgcc gaaacgagga
     8581 tgggcacctt tatgtttccg catgtcgatc aattcatggg ctttcttgac ggcgttgaag
     8641 gccgcttcga gcaccggctc cggttccccg accatcgtca cgacggtgcg gttggtgtcc
     8701 cagccgggat cgacatcgag cagtttcgcc ccatttacct tgcagatctc tgcggtgatc
     8761 tggtcgatga cgacgcgatc acgaccttcg ctgaaattgg gtacacattc gatgatgcga
     8821 gccatgagaa cctccgattg ttcgtctttg ttcagttcat cctgtggtga acctagagaa
     8881 gggaaccagg taagtcaaga aaacctagag tgtacggggt cggagaaaag agtccccaag
     8941 aaaaaggcct caaggggtcg cggtctcggg ttcttccggc tcttggggct gctcgatcgg
     9001 acaacattcg tagcccggga aaagagaacg gatcagatcg tccgcatccc cgaaagtata
     9061 acagtccaaa agagctccgt cctgtcccgg gaaacagcct tccaagtggg aactcgggaa
     9121 atagcagtat tccatgatca catcatatat ctcctggtca cgctcgtact tcgcctgctc
     9181 atatgccgcc tcgaccggat actgctcctg cagttcctcg tcggtcggcc aatcgtcatt
     9241 gaccggatag accgagccca tcatgaccga aaggccatag agcggcacaa ggctgaaacg
     9301 cagtttgttg gtgcggatgc gatcgccacc accgagctta ataccttcgc cctgcacatc
     9361 gacgatgatg gggtaggtct cccaatcgaa aatgacacca gcattgaaca gagtggtgaa
     9421 tatgttccta agattagtat gctgtgccgt ggtgaaaaga tcgaccgctc cggcgatctt
     9481 gcccccctcg ggatctatga ccacacccga ctcgaccgta cgggtagccc acgcaccgcc
     9541 atcgacaccg gggaccggcg gcaattcgta ctcaaggatc aggcgttcga gcctccaccg
     9601 gttggcctca tgaatgtcat agggctgttc ggggttcata tctccagtct gcgatgaaac
     9661 attcatatag ttgaccatct tgaagggcaa caggtactgc tgggtgaagt agatatccat
     9721 gtagccgatg tcataccaga ggttggaacc gatctccaat ttgcaggagt tctgttcggt
     9781 cagcggtatg aacttttcga ggaccaccga ctgttcgcta ttggcacagg cgaccgtaag
     9841 gaacagagcc gccacggcgg caagaaccac gcgtttcatg gctttctctc cattatatct
     9901 catggcgtta ttcctcctcc gatctcgtca gcggcatcgc ggagcgaacc ttgttcatga
     9961 tgcgcggcgt gatgaagatg agcagttcgg aacgctcgac cctttcgtcg atccgcttga
    10021 aaagccatcc caatatcggg atgtctccca gcaccgggac cttcctgacc gtcttactca
    10081 ccttgttggt gtagaccccg ccgatgacga tggtgtcgcc gctccgcacg aggacctcgg
    10141 tctgcgcctc ttttttcatt atagccgact cacccaagta gttggcgttt gaatagtcgg
    10201 cctccgactt attgatattg agtttcatga ggatggagcc gtcggaggtg atgtgcgggg
    10261 tgctcttgag aataagcgtc gcatcgacga atttcgtcgt gacctcgcct cccgtcgagc
    10321 gggtggtgat cggtacgctg ataccctgct ggatcatcgc ttcggag
//
LOCUS       DAOQUE010000034        10169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_7403_length_10169_cov_4.189045, whole genome shotgun sequence.
ACCESSION   DAOQUE010000034 DAOQUE010000000
VERSION     DAOQUE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7403_length_10169_cov_4.189045"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /gene="hcp"
                     /locus_tag="PKH10_01950"
                     /gene_synonym="priS"
     CDS             complement(<1..1609)
                     /gene="hcp"
                     /locus_tag="PKH10_01950"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNT26918.1"
                     /translation="MSMFCHQCQETARNKGCDKQQGVCGKSETVANLQDLLIYTMKGI
                     GFYGHELAKVGHYDEKGAFFVVKGLFATITNANFDEKDFVRWIKEGQEIKNRFKGECE
                     KRGMKFATVPPEAAWSPKNDDEIQAAAASGRGGWLTIENEDVRSLRAIILYGLKGMSA
                     YGEHAYALGFHKKEIFEFIFEGLATIIDDSRSAGDLTAFVLKTGAEGVAVMALLDEAN
                     TTTYGHPVITEVKTGVRGNPGILVSGHDLRDLHDLLEQTKGSGVDVYTHSEMLPAHYY
                     PFFRKYDNLVGNYGGSWWHQKEEFEKFNGPVLLTSNCLVPPKESVKGRIFTTGVVGFD
                     GMTHIADRELGKMKDFSKLIALAKTCKAPEKLEDGKIVGGFARTQVLAVADKVVAAVK
                     SGAIKRFVVMAGCDGRHKERDYYKKVAEALPKDAVILTAGCAKYQYNKLPLGDIGGIP
                     RVLDAGQCNDSYSLAVIALKLKEVFGLDDINKLPISFDIAWYEQKAVLVLLALLHLGV
                     KGIRLGPTLPGFLSPAVAKVLVENFDIK"
     gene            complement(1663..2568)
                     /locus_tag="PKH10_01955"
     CDS             complement(1663..2568)
                     /locus_tag="PKH10_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013245087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT26919.1"
                     /translation="MAKRKIIKIDNDKCTGCGLCVPNCAEGALQIIDGKARLISDLFC
                     DGLGACLGHCPEAAITVEEREAEPYNERLVMEKNIIPAGRNTIRAHLLHLRDHGAMDL
                     LQEAFAVVREHGMDPKEIVHGTPDGDACGCGDHGKKEHHSHHHGGGCPGSKVVDLKKT
                     MHGGHHEKGRAGGDAPTGRIESELKNWPVQIHLASPYAPYFKEADIVVIADCVAYAYA
                     ETHREFIKDKVVLIGCPKLDDAAAYVDKLSEIIRAGKPKSIKVVRMEVPCCGGMIEIV
                     KQAIKNSGEVMPFETVTISIDGRKL"
     gene            2785..4818
                     /locus_tag="PKH10_01960"
     CDS             2785..4818
                     /locus_tag="PKH10_01960"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNT26920.1"
                     /translation="MLTNLDISQAMTVKLDAVLPPAPVFEPGIRRAPKRELTLTRSEI
                     ALALRNALRYVPEELHETLAPEFLDELITNGRIYGYRFRPPGNLSGRPIETYTDIYTE
                     GKAFQVMIDNNLDFATALYPYELVTYGETGQVCQNWMQYRLIKKYLEVLTQEQTLVIH
                     SGHPLGLFKSKPDAPRVITTNGLMVGLFDNPTDFNRAAALGVANYGQMTAGGWMYIGP
                     QGIVHGTYNTLLIAGRLKLGVAGDGDLAGKLFVSSGLGGMSGAQGKACWIAGGVALVA
                     EVDKSRIDTRHSQGWVTHVTGTAQEAFDLALDLAKKKERAAIAFHGNIVDLLEYAAKE
                     KIAIDLLSDQTSCHAVYEGGYCPQGLSFAERTDMLAKDKQKFIGLVNKSLRRHYELIR
                     ELTACGTYFFDYGNAFMKSVFDAGVTEIARNGVNTYDGFIWPSYVEDILGPELFDYGY
                     GPFRWVCLSGKEEDLIKTDAAAAEYISTCREPLRYQDRDNYLWVRDAMKNKLVVGTKA
                     RILYQDAWGRTALALKFNKMVRDGVVGPIMLGRDHHDTGGTDSPFRETSNIKDGSNVM
                     ADMATHIFAGNAARGMSLIALHNGGGVGIGKSINGGFGMVLDGSARVDTILSTAMPWD
                     VMGGVSRRAWARNEHAIETSAQYNRENAGRDHITLPFIAQDELIEKTIAAKMK"
     gene            complement(4821..5618)
                     /locus_tag="PKH10_01965"
     CDS             complement(4821..5618)
                     /locus_tag="PKH10_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT26921.1"
                     /translation="MTSAQLTALAIFLLFSTLFGMFGIGGGAIYTPVQLYLGTPFNEA
                     ATVSLFLIAVTSISCGVVYVRAHLIDTPSVAALAVFSFTGSLCGGWSAGRVPEEVLAG
                     LFAFFLLVNGAHLAFSDIREGHCEVSSLPRRLLWERTIAGERVVINLPLAIPIAFLSG
                     IFGGMVGLGGGIVMLPLMVTILRFPIKVAIATSSFLALATGIGGLLTHLTHTTAPWGF
                     MIPAALMVGIGSAIGARWTVQSHSKRIRRLLGVFFVAIAVLLFVRGY"
     gene            complement(5615..6874)
                     /locus_tag="PKH10_01970"
     CDS             complement(5615..6874)
                     /locus_tag="PKH10_01970"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT26922.1"
                     /translation="MRAVIPTLLCLLLPILLIAEDTALPIGDIIKEKALSTDVDEPKK
                     ATPRLEDITLYEMNDSVRVVVRPSEKVDFKYDLLAGNAKDRIYIDLKNGLTAHGFTAP
                     QVSADSFLKAIRLGKREEGTRIVLDTGKVEKYNVMVMDDPWRIVIDYIGARQTPPAEK
                     KSGPAVAGPEKKSAEKQVAQKTAVAPTPEKKEKRFIIVVDPGHGGKDPGAIRKNLQEK
                     DIVLALAKTVRRIAAKKHPDIEVVLTRDDDRFIPLEERAIIANRHDGDLFISIHTNTY
                     KAKEIAGVELYHLNNRSDEYTDKLTRVENQITNTNSFLNTILVDMTMSLYIGDSQKFA
                     ESIGKRWQETLAPFETKIRDWRKGALFFVLVGARMPSLLVEVGFLSNPREAKILQKEK
                     YLEALATGLLDAVAEVREKHTLAKDRP"
     gene            complement(6877..8289)
                     /locus_tag="PKH10_01975"
     CDS             complement(6877..8289)
                     /locus_tag="PKH10_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26923.1"
                     /translation="MRSIAIALSLLLPLWAGAVDFDFDRFLERFDETKFIGAGAGFSH
                     EYDDELFGVSIFGDLRWEPAGFGFNVPLRWLVHDGNGDTDTVAGLPTYDWDEARDYVR
                     LITYAQYGHHEDEVYLYFGDHENRYIGHGTLLGGYFNNIRLNHPSRGVYALLQTEWAG
                     GELFLSDVAPPDIIGGRAFIKPASFFDRDSYANNLEVGVSWFSDIFMPETIELDQQTL
                     ARSANRVFDSAVGLDLSFRALALRWWHLTPYMDWNLLRFGGNGLHFGLRQHFDIEVKD
                     DTLSILTTVESRVYQRNYSPQYFGTFYDIEKERYYGGETKYWVHSSKNNLGAGPWYTG
                     WYGDIVLYMVGSFSIGGSFEWNEYLSNQHLDTADQGVRRGRFTANLFGDLILFDRLRL
                     GLFFTRADAGRGGFFDLADHPSLLSVSASYAINEYFSASAHCSNGYRLHIYLDPNGTL
                     RDRYRSEIQFGLGFSAGYGF"
     gene            complement(8321..9256)
                     /locus_tag="PKH10_01980"
     CDS             complement(8321..9256)
                     /locus_tag="PKH10_01980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT26924.1"
                     /translation="MRSALPLLLVPAFLVWHPLGAAERVLNTIAIVVNDGYLTLYDVK
                     KEIAPKAPDAVTDGQIAERKKEITDKLVGDLLVKQEIKKRNITVSDAELDLAIENVAE
                     RNRITVDKLKEEIAGQGISWDTYRNEVLRRQLEILSLKRDIAMATLDVDETTLRDLYR
                     RHFTGGVIYSASHIVLQVVQGASDADIYDRIVDIHRAVQGGKLTFEEAAQRYSEDAAA
                     RNGGKLPSFRFNEMDPKFSEEVAKLDPGEMSRPFRSRFGWHIVRLDDVEKSDPPPYEQ
                     VRDKVRYLFYQMNQEKAFESWIKAKRQSSAVKVLF"
     gene            complement(9262..>10169)
                     /locus_tag="PKH10_01985"
     CDS             complement(9262..>10169)
                     /locus_tag="PKH10_01985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT26925.1"
                     /translation="YMKARYNTPEKKEEFVGKIIEGEILAREAIRKGVMSDPILLTKI
                     KSTVARHYQSVTLQKEIEANIKITDEDLKKYYEEHQKEFIQPEKVKASHILVKFDKNK
                     AGDREAKLAQAKKVLAEVKAKAKDPKSFNDLAGKYSEDEGSKRRGGDVGFFERTEEGG
                     RMAKEFSEAAFALKNIGDISDVVESPFGFHIVQLTARREKVEKPFDEVKARIESTLKA
                     EKRKGVYEESIEKIKKDLGFKFNKESLEKLDMGVPEKVEQASREFDKNKPEGAPAPVP
                     APIKPIQPMKPQEIPQPATPPAPQQ"
BASE COUNT         1876 a         3020 c         3153 g         2120 t
ORIGIN      
        1 gcttgatatc gaagttctcg acgagcacct tggcgaccgc gggcgacagg aagccgggaa
       61 gcgtcggccc gagccggata cccttcacgc cgaggtggag gagcgcgagg aggaccagca
      121 ccgccttctg ttcgtaccac gcgatgtcga aactgatggg tagtttgttg atgtcgtcga
      181 gtccgaagac ctccttgagt ttgagcgcga tgacggcgag cgaataggaa tcgttgcact
      241 gaccggcgtc cagcacgcgc gggatgccgc cgatgtcgcc gagcggcagc ttgttgtact
      301 ggtacttcgc gcagccggcg gtgaggatga ccgcatcctt cggcagcgcc tcggcgacct
      361 ttttatagta gtcgcgttcc ttgtggcggc cgtcgcagcc cgccatgacc acaaagcgtt
      421 ttatcgcccc cgacttgacc gccgcgacca ccttgtcggc gactgcgagc acctgtgtcc
      481 gggcgaagcc gccgacgatc ttcccgtcct ccagtttctc gggcgccttg caggtcttgg
      541 caagggcgat gagtttcgag aagtccttca tcttgcccag ttcgcggtcg gcgatgtggg
      601 tcatgccgtc gaagccgacc accccggtgg tgaagatgcg ccccttgacc gactccttcg
      661 gcggaacgag gcagttcgag gtcagcagca ccgggccgtt gaacttctcg aactcctctt
      721 tctggtgcca ccacgagccg ccgtagttgc ccacgaggtt gtcgtacttg cggaagaagg
      781 gatagtaatg ggccggcagc atctcggagt gggtgtagac atcgacgccg ctccccttgg
      841 tctgttcgag gaggtcgtgc aggtcacgca ggtcgtggcc gcttacgagt atgccggggt
      901 tcccgcggac gccggtcttg acctcggtaa tgaccgggtg gccgtaggtg gtggtgttcg
      961 cctcgtcgag aagcgccatc accgcgaccc cctccgcgcc ggtcttgagg acgaaggcgg
     1021 tcagatcgcc cgccgacctg gagtcgtcga tgatcgtcgc gagtccttcg aagatgaact
     1081 cgaagatctc tttcttatgg aagcccagag cgtaggcgtg ttcgccgtag gcgctcatcc
     1141 ccttgagccc gtacaggatg atggcgcgga gcgaccggac atcctcgttc tcgatggtga
     1201 gccagccgcc gcgacccgat gccgccgccg cttgtatctc gtcgtcgttc ttcgggctcc
     1261 acgccgcctc gggaggaacg gttgcgaact tcatgccgcg tttttcgcat tcgcccttga
     1321 agcggttctt gatctcctgc ccctccttga tccagcggac gaaatctttc tcgtcgaagt
     1381 tggcgttggt gatggtcgcg aagagtccct tcacgacgaa gaaggcgccc ttctcgtcgt
     1441 agtgaccgac cttcgccagt tcgtggccgt aaaagccgat ccccttcatc gtgtagatga
     1501 gaaggtcctg cagattggcg accgtttcgc tcttgccgca gaccccctgc tgtttgtcgc
     1561 accctttgtt gcgggccgtt tcctgacatt gatgacagaa catactcatg atctcctcct
     1621 aaaaaaatgg ttgccggatc cccaaggcac gatatcgtat cctcaaagtt ttcgtccgtc
     1681 aatagaaatg gtgaccgttt cgaagggcat cacctcgccc gaattcttta tcgcctgctt
     1741 cactatctcg atcatccctc cgcagcaggg gacctccatc cggaccacct tgatgctttt
     1801 cggtttcccg gcgcggatga tctcgctcaa cttgtcgaca taggcggccg cgtcatcgag
     1861 tttcggacag ccgatgagca ccaccttgtc cttgatgaac tcgcggtgcg tttcggcata
     1921 ggcgtaggcg acgcaatcgg cgatgaccac gatatcggcc tccttgaagt agggggcgta
     1981 ggggctcgcg aggtgtatct gcaccggcca attctttaac tcggactcta tccgaccggt
     2041 aggggcgtcg ccaccggcac gccctttttc atggtgtccg ccgtgcatcg tctttttcag
     2101 gtccaccacc ttcgagccgg ggcagccgcc gccgtgatga tggctgtgat gttccttctt
     2161 gccgtggtcg ccgcagccgc aggcgtcgcc gtccggtgtg ccgtggacga tctccttcgg
     2221 gtccatgccg tgttcgcgaa cgaccgcgaa ggcctcctgc agcagatcca tcgcgccgtg
     2281 gtcgcggagg tggaggaggt gagcgcggat ggtgttacgg cccgccggga tgatgttctt
     2341 ctccatcacg agccgttcgt tatagggctc cgcctcgcgc tcctcgacgg tgatcgccgc
     2401 ctcgggacag tggccgagac atgcccccag accatcacag aacaggtcgc ttatcaaccg
     2461 agccttcccg tcgatgatct ggagcgctcc ttcagcgcag ttgggtacgc acagcccgca
     2521 gccggtgcat ttgtcgttgt cgatcttgat gatctttcgc ttcgccatga caacctccgt
     2581 cttgtcaata aggactcctg tgtctagaat agcaggcaaa aatacaaaat caataccttg
     2641 tggtccttaa tccgaataaa agatttaagt taccgttatt atgaatatat attttcctta
     2701 tcggcttgaa cgatgctttc ttgaaatcga cggctcctca cgctatagtc gggcgatatg
     2761 atgtggaacc gatggaggat accgatgctc acgaacctcg acatctccca agcgatgacg
     2821 gtgaaactcg atgcggtgct tccgccggcg cccgttttcg agccggggat ccggcgcgca
     2881 ccgaaacgag aactgacgct caccagaagc gagatagccc tcgcgctccg caacgcgctc
     2941 cgctatgtgc cggaagagtt gcacgaaacg ctcgcccccg aattcctcga cgaactgatc
     3001 accaacgggc gcatctacgg ctaccgcttc cgaccgccgg gtaacctttc ggggcggccg
     3061 atagagacat acaccgatat atacaccgaa ggaaaggcct ttcaggtgat gatcgacaac
     3121 aatctcgact tcgccaccgc cctctatccg tacgaactgg tcacctacgg cgaaacgggg
     3181 caggtctgtc agaactggat gcagtatcga ctcatcaaga aatatctcga ggtcctcacg
     3241 caggagcaga cgctcgtcat tcacagcggc catccgctgg gactgttcaa gtcgaaaccc
     3301 gatgccccgc gcgtcatcac caccaacggg ctcatggtgg ggctcttcga caatccgaca
     3361 gacttcaacc gggctgcggc gctgggcgtt gccaactacg gccagatgac cgccggtggc
     3421 tggatgtaca tcgggccgca ggggatcgtc cacgggacct acaatacgct cctcatcgcg
     3481 gggcggctga aactcggcgt ggcgggcgac ggcgatctcg ccggaaaact gttcgtctcg
     3541 tcggggctcg gcgggatgag cggggcgcag gggaaagcct gctggatagc gggtggtgtc
     3601 gcgctcgtcg ccgaggtcga taagtcgcgc atcgataccc gtcattcgca ggggtgggtg
     3661 acccatgtca ccggaacggc gcaggaggcg ttcgacctcg ctcttgacct tgcgaagaaa
     3721 aaagagcggg cggcgatcgc gttccacggc aacatcgtcg atctgctcga atacgcggcg
     3781 aaagaaaaga tcgccatcga cctcctttcg gaccagacct cgtgccacgc cgtttatgaa
     3841 gggggctact gtccgcaggg cctttcgttc gccgagcgga ccgatatgct cgcgaaggat
     3901 aaacagaagt tcattggact ggtgaacaag agtctgcgcc gccattacga actcatccgg
     3961 gaactcaccg catgcggcac ctactttttc gactacggta acgccttcat gaagtcggtc
     4021 ttcgacgccg gggtgaccga gatcgcccgc aacggcgtca acacctacga cggcttcatc
     4081 tggcccagtt atgtcgagga tatcctcggc cccgaactgt tcgactacgg ttacggcccg
     4141 ttccgctggg tctgcctgtc gggcaaagag gaggacctca tcaagaccga cgcggcggcg
     4201 gcagaataca tcagcacctg ccgcgaaccg ctccgctatc aggaccgtga caactatctc
     4261 tgggtgcgcg acgcgatgaa gaataaactg gtcgtcggca ccaaggcgcg gatactgtat
     4321 caggacgcgt ggggtcggac cgcgctcgct ctcaagttca acaagatggt gcgcgacggc
     4381 gtcgtcggtc cgatcatgct cggccgcgac caccacgaca ccggcggcac cgacagcccg
     4441 ttccgcgaga catcgaacat caaggacggc tcgaatgtca tggccgacat ggcgacccac
     4501 atcttcgccg ggaacgcggc gcgcgggatg agcctcatcg cgctccacaa cggcggcggg
     4561 gtcgggatcg gcaagtcgat caacggcggt ttcggcatgg tgctcgacgg ctcggcgcgg
     4621 gtcgacacga tactctcgac cgcgatgccg tgggatgtca tggggggcgt ctcgcgccgt
     4681 gcgtgggcga ggaacgaaca cgccatagag acaagcgccc agtacaaccg cgagaacgcc
     4741 ggccgcgacc acatcacgct cccgttcatc gcgcaggacg aactcatcga aaagacgata
     4801 gcggcgaaga tgaagtagcg ctagtagccc cggacgaaca gcaacacggc gatagcgacg
     4861 aagaacacgc ccaaaaggcg ccgtatccgc ttcgagtggc tctgcaccgt ccatcgagca
     4921 ccgatcgccg acccgatccc gaccatgagc gcggccggta tcatgaagcc ccacggcgcc
     4981 gtggtgtggg tcaggtgggt cagcaggccg ccgatgccgg tggcgagtgc gaggaaggaa
     5041 ctggtcgcga tggcgacctt gatggggaac cgcagtatcg tcaccatgag tggcaacatc
     5101 acgatgccgc cgccgagacc gaccatgccg ccgaatatgc ccgacaggaa ggcgatgggg
     5161 atcgcgagcg gcaggtttat cacgacccgt tcacccgcta tcgtccgttc ccacaggagg
     5221 cgacgcggca gagaggagac ctcacagtgt ccctcgcgga tgtctgaaaa ggcgagatgg
     5281 gcgccgttca cgagcaggaa aaaggcgaac agtcccgcga gcacctcttc aggcacccgt
     5341 ccggcgctcc agccaccgca gagactgccg gtgaaggaga acacggcgag cgcggccacg
     5401 ctgggggtgt cgataaggtg cgcccgtaca taaacgaccc cgcaggagat cgaggtcacc
     5461 gcgatgagaa aaagactgac ggtcgccgct tcgttgaacg gcgtgccgag gtagagttgc
     5521 accggcgtgt agatcgcgcc gccgccgatg ccgaacatgc cgaagagggt cgagaataga
     5581 aggaatatgg cgagagcggt aagttgcgcg gaggtcatgg gcggtccttg gcaagcgtgt
     5641 gtttctcgcg tacctccgcc acagcgtcga gcagtcccgt cgccagcgcc tcaagatact
     5701 tctctttctg aaggatcttc gcctcgcgcg gattgctgag gaacccgacc tctacgagga
     5761 gcgacggcat gcgcgcgccg accagcacga agaagagcgc tccttttcgc cagtcgcgta
     5821 tcttggtctc gaacggcgcg agcgtctcct gccagcgttt accgatgctt tccgcgaatt
     5881 tctgcgaatc gccgatatag agactcatcg tcatgtccac gaggatggta ttgagaaagg
     5941 agttcgtgtt ggtgatctgg ttctcgacgc gggtcagttt gtcggtgtac tcgtcgcttc
     6001 ggttgttcag gtggtagagt tcgacgccgg cgatctcttt tgccttgtag gtgttggtgt
     6061 gtatcgatat gaacaggtcg ccgtcgtgcc ggttggcgat gatggcccgc tcctcgagtg
     6121 ggatgaaccg gtcgtcgtcg cgggtgagta cgacctcgat gtcggggtgt tttttggcgg
     6181 cgatgcggcg aacggtcttg gcgagcgcga gcacgatatc cttttcctgc aggttcttgc
     6241 gtatcgcgcc gggatccttg ccgccgtgac ccggatcgac gacgatgatg aatcgcttct
     6301 cttttttctc cggcgtcggc gcgacggcgg tcttttgcgc cacctgtttc tccgccgact
     6361 ttttctccgg tcccgcgacg gccggtccgc tctttttctc ggcggggggc gtctgcctcg
     6421 cgccgatata gtctatgacg atgcgccacg ggtcgtccat caccatgaca ttgtatttct
     6481 cgaccttgcc ggtatcgagc acgatgcggg tgccctcttc ccgtttcccc agccggatgg
     6541 ccttcaggaa cgaatcggcg gagacctgcg gtgcggtgaa gccgtgtgca gtgagcccgt
     6601 tcttgaggtc tatgtagatg cggtccttgg cgttccccgc gagcaggtcg tacttgaaat
     6661 cgaccttttc ggaaggccgg acgaccacgc ggaccgagtc gttcatctca tagagcgtga
     6721 tgtcctcgag tcggggggtg gcttttttcg gctcgtccac atcggtcgac agcgcttttt
     6781 ctttgatgat gtcgccgatc gggagcgccg tatcttcggc gatcagcagg atcgggagaa
     6841 ggaggcacag gagcgtcggt ataacggcgc gcatcgtcag aagccgtacc cggcggagaa
     6901 cccgaggccg aactgtatct cggagcggta gcggtcccgg agcgtgccat tggggtcgag
     6961 gtagatgtgg agccggtaac cgttcgaaca atgggccgag gcggagaaat actcgttgat
     7021 ggcataggag gccgagacgg agaggagcga cgggtgatcg gcgaggtcga agaacccgcc
     7081 gcgcccggcg tcggcccgcg tgaagaacag cccgagccgc aggcgatcga acaggatgag
     7141 atcgccgaag agattcgcgg tgaaccggcc gcgccggacc ccctgatcgg cggtgtcgag
     7201 atgctggttg gaaaggtatt cgttccactc gaaggagccg ccgatggaga aggaaccgac
     7261 catgtacagc acgatatcgc cgtaccagcc ggtgtaccac ggacccgcac caaggttgtt
     7321 cttcgaggaa tggacccagt atttggtctc gccgccgtag tagcgctctt tctcgatgtc
     7381 gtagaaggtg ccgaaatact gtggcgagta gttccgctgg tacacgcggg actctaccgt
     7441 ggtcaggatg gagagcgtgt cgtccttcac ctctatgtcg aagtgctgcc ggagaccgaa
     7501 atggagcccg ttgccgccga agcgtagaag gttccagtcc atgtaggggg tgaggtgcca
     7561 ccagcgcagg gcgagtgcgc ggaaggagag gtcgagcccg accgccgagt cgaatacgcg
     7621 attcgccgac cgcgcgagcg tctgctgatc aagttctatc gtttcgggca tgaagatatc
     7681 ggagaaccaa ctgacaccca cctcgaggtt gttcgcatag gaatccctgt cgaagaaact
     7741 tgccggcttt atgaaagcgc ggcccccgat gatgtcgggc ggcgccacat cgctcaggaa
     7801 cagttcgccc ccggcccatt cggtctgcag gagcgcgtag acgccgcggc tcggatggtt
     7861 gagccgtatg ttgttgaaat agccgccgag cagggtgccg tggccgatgt aacggttctc
     7921 atggtcgccg aagtagagat agacctcatc ctcatggtga ccatactggg cgtaggtgat
     7981 gagccgcaca tagtcgcgcg cctcgtccca gtcgtaggtc ggcaggccgg cgacggtgtc
     8041 ggtgtcgccg ttgccgtcgt gtacgagcca gcggagaggg acattgaagc cgaacccggc
     8101 cggttcccag cggagatcgc cgaagatcga gacgccgaac agttcgtcgt cgtactcgtg
     8161 cgagaacccg gcccccgcgc cgatgaattt ggtctcatcg aaccgttcga ggaaacggtc
     8221 gaaatcgaaa tcgaccgcgc cggcccagag gggcaacagg agcgacaacg cgatggcgat
     8281 cgatctcatg cggcgtctcc cggtacgcgg caggccacgg ctagaacagc accttcacgg
     8341 cgctcgactg ccgtttggcc tttatccaac tttcgaaggc cttttcctga ttcatctgat
     8401 aaaaaagata gcgcacctta tcccggacct gttcgtaggg gggcgggtcg ctcttttcca
     8461 catcgtcgag tcgcacgata tgccagccga aacgggagcg gaaggggcgc gacatctcgc
     8521 cagggtcgag tttggcgacc tcttcggaga atttcgggtc catctcgttg aaacggaaac
     8581 tcggcagttt accgccattg cgggccgccg cgtcctccga atagcgctgg gccgcctcct
     8641 cgaaggtgag tttgcctccc tgcacggcgc ggtgtatatc cacgatacga tcgtagatgt
     8701 cggcgtccga agcgccctgc acgacctgca ggacgatgtg cgacgcgctg tagatgaccc
     8761 cgccggtgaa atggcgtcgg tacaggtcgc gcagggtggt ctcgtccaca tcgagcgtcg
     8821 ccatcgcgat gtcgcgcttg agcgagagta tctcgagttg ccggcgcagg acctcgttgc
     8881 ggtaggtgtc ccacgatata ccctgcccgg cgatctcctc cttgagtttg tccacggtga
     8941 tgcggttgcg ttccgcgaca ttctctatgg cgaggtcgag ttcggcgtcg ctcacggtga
     9001 tgttacgctt ttttatctcc tgcttcacca gcaggtcgcc gaccagtttg tcggtgatct
     9061 ctttcttgcg ttcggcgatc tggccatcgg tcaccgcgtc gggggctttc ggcgctatct
     9121 ccttcttgac atcgtagagc gtaagatagc cgtcgttgac cacgatggca atggtgttga
     9181 gcacccgttc ggcggcgccg agcgggtgcc agaccagaaa ggcgggtacg agcaacaggg
     9241 gaagcgccga gcgcatcggg gtcactgctg cggagcgggg ggcgtcgccg gctgggggat
     9301 ctcctgcggc ttcatcggtt gtatcggttt gatgggagcg ggaacgggcg ccggggctcc
     9361 ttccggttta ttcttgtcga attcgcgcga agcctgttcg accttctcgg gcacacccat
     9421 gtcgagtttc tcgagcgact ccttgttgaa tttgaagccg aggtccttct ttatcttctc
     9481 tatcgattcc tcgtacaccc ctttgcgttt ctcggccttg agggtcgatt cgatgcgggc
     9541 cttgacctcg tcgaagggtt tttcgacctt ctcgcggcgc gcggtcaact ggacgatgtg
     9601 gaagccgaag ggggattcga ccacgtcgct gatgtcgccg atgttcttga gcgcgaacgc
     9661 ggcttcggag aattccttgg ccatccggcc gccttcctcg gtccgttcaa agaagccgac
     9721 atcgccgccg cgacgcttgc tcccctcgtc ctcggagtat ttgcccgcga ggtcgttgaa
     9781 cgacttggga tccttcgcct ttgccttgac ctcggcgagc acttttttcg cctgagcgag
     9841 tttcgcctcg cggtcgcccg ccttgttctt gtcgaatttg acgaggatgt ggctcgcctt
     9901 cactttttct ggctgtatga attctttctg atgctcctcg tagtacttct tgaggtcctc
     9961 gtcggttatc ttgatgttcg cctcgatctc cttttggagc gtcacgctct ggtagtggcg
    10021 cgcgacggtc gatttgatct tggtgagcag tatcgggtcg ctcatgacgc ccttgcggat
    10081 agcctcgcgg gcgagtatct cgccttcaat gatcttaccc acgaactcct ctttcttctc
    10141 cggcgtgtta tagcgcgcct tcatgtagg
//
LOCUS       DAOQUE010000035        10121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_7447_length_10121_cov_4.152792, whole genome shotgun sequence.
ACCESSION   DAOQUE010000035 DAOQUE010000000
VERSION     DAOQUE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7447_length_10121_cov_4.152792"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3465)
                     /locus_tag="PKH10_01990"
     CDS             complement(<1..3465)
                     /locus_tag="PKH10_01990"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HNT26926.1"
                     /translation="MKKNATMLIALFLLFLTACDGSSKPSVDDNGGNDELTPESDKTL
                     PETDTTATDDAPLDGDSAQPDDSDTMPEGDTQPDEDEAYPDEVIPDEMVDEAWDGGDE
                     AMFDDMTDEDIITVPDVVESEDDTLPFDDGTPDTNDIFPDQFDAFTGDDDILLTDTDE
                     LVTCEDDGQDCTEEVVLNDQCLHLPRHELCDAAEMCNPTDGCVIADGWLCRSCAGGLP
                     CAYSTDICASLLGTSVCLTACVSDEECLPSFSCLDIYDEEDNFLGRGCKPDSQICCVD
                     FDGDMAGVGAMCQLQDCDETDPDVRPGMTEVCNGIDDDCSGTADDNLQSSAPFCLKQQ
                     GVCFGSRQSCGGMAGWLPCGTGDYLAWDSRYETVEATCDLADNDCNDQIDEPFEEVLY
                     KSCTEGDGICERSGFTVCDEPGTGVICNAEKGLPDPEGEKCNNLDDDCDGEIDNGFDE
                     KNTVCTDGYGVCRVTGIYVCKTDGSDTECNVKALPSTDDEDCDGLDNDCNDAVDDGLT
                     GGLCPNQKGVCAGTRQSCGGFSGWLPCGPTDYANQTNGTYEQSETICDGKNNDCDDET
                     DENVASYAPACPLQSGVCKGSRQTCGGISGWLACTAVNYGPEYETTETQCDYLDNDCD
                     TQIDEGFKNQVTGKYDQNTACGNCSTDCTAIYNKPNGYGTCNATGSPVCWLTCNVNYY
                     NLNNVPDDGCEFLLETSVIYVSTSNGTNDTTCGLGPSGTVVGYYPCKTIAYGISRAQA
                     TTRTKVYVADGLYEETVTLVNGISLYGGYKADTWERHTASSLTVWRGNDTATHKRTVI
                     ASGITSSTVVEGFVLYGQAATAAGGNSYVFHITGSDADLDIRDNTIYGASGGPGTDGT
                     AGTVVSNATSGTSGPNSYETSSDSCTASRQNTNRGTNASCSNANGGNGGGNACPPSDN
                     TQNSGVTGFAGEGSGGGDGGEEGYDGRFFKSGTSTVCDDYALDGPMEGLNGENGASGT
                     EGVPGSGALNATGGISGSNWIGASGLGGGSGGAGGGGGGGGGGGGGDDSGGTAYYDTL
                     GGHGGGGGAGGCGATGANYGTAGGGSFALFLISTTAPTLQNNTIFLGAGGAGGKGGNG
                     GVGGAGGNGGDGGTGRFCGGAGGKGGKGGRGGHGGGGGGGAGGVAFGIAMSGVTGAPD
                     YTMNNTFSG"
     gene            complement(3476..7066)
                     /locus_tag="PKH10_01995"
     CDS             complement(3476..7066)
                     /locus_tag="PKH10_01995"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HNT26927.1"
                     /translation="MRRFLLDLLRCGFSLLLLTVLACSSSKPQSSKDEDTTGDGDSLL
                     ADRDALTDEILPTDEETDVTPDTEGSAPDGDTTVGDDAVPVDDGADIFDDDELFVDEA
                     TDIDEPIVDEEQEEDDPDEEYPDEAVVEDEFVADDAWVLPDIDSPVGCNDQITCTEDL
                     IIGGQCKHLPRHEWCEPGLLCNKTDGCVEPDEWVCRSCESAPCPYDGDICAPLLGEKL
                     CLLPCDTDAECPGDFRCNDLFDLQGLLLGRGCAPTNFVCCLDIDGDNAGVGAKCALYD
                     CDESSAQRYPGAIEQCNGADDDCDLIVDNNLGTAPLCAKQGGICQGLRKTCGGTAGWL
                     ACTTAQYLAWNDLYQETEALCDGVDNDCNGVTDDPFATELFEICTDGIGECKRAGFMV
                     CNDTHDGVVCNAVKADPASEELCNNKDDDCDTFIDNDSRFEQKGEVCFVGTGACLRAG
                     IKICNGDGDGLDCSVTQGTPTDEECNGIDDDCVLGIDNGVEASAPQCALTAGVCSTAR
                     KKCGGTASWLDCGYGDYLAASGGTYEVSETLCDGLDNDCIGGVDNNLTNYAPACDNPV
                     GPAQPLRGVCVGARKACGGASSWLACGAAQFGADYQATENRCDYLDNDCDGELDEGFL
                     NAGTGKYDQNTACGNCATNCTTIYAKPNAEGWCDATGTPECKMRCTNTSLYFDLNGVP
                     DDGCEFTLDVDAIYVSESNGDDLDPNCGLGPVAIGQKPCATIAKGLQRAGAPLNRSKI
                     FVADGTYEETVILTPGKSLYGGYRPDTWGRHAPTTMTIIRGNTFDYEPHKATIIASAI
                     TATTVLDGFVLYGQSATGTGGNSYVIYVTGSNASLTISNNVIYGAMGGPGASMGQANA
                     GAPGEDGGGRPADGAGYDPVSANGRDSGCTITRQSNNGGSSSCGVAGGRGGGNYCAPS
                     YNNYGSSRVGDNGSGSGAGVGGGRGYDGRVHTVTYNPPYNMDCTIPSGSMNGTNGTNG
                     GSGTDGSGGTGCSSTNGSVVSGHWGGAGGNAGGVGTAGGGGGGGGSGGGGDSDISGEN
                     DVFGSHGGGGGAGGCGGTGASGGAAGGGSFAIFVTGSTGAVVLRDNAITLGSGGRGGN
                     GGTGGSGGEGGIGGAGGNTLNTPPLNCGGVGGAGGNGGNGGAGGGGGGGCGGTAVGIG
                     YSGTAPNFATGNTYYGNTPDKGGAGGAGGSSMGLPGSAGSIGAVYTTRGY"
     gene            7242..7787
                     /locus_tag="PKH10_02000"
     CDS             7242..7787
                     /locus_tag="PKH10_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HNT26928.1"
                     /translation="MGQENFREITPAEMTDNPFTAFGKGWALLAAGKGKKSNMMTIAW
                     GALGVLWHREVCFVFVRPPRHTYQLTEAEPCFSVGFFPEQYRDVLQLCGSISGAKEDK
                     IAASKLTLLDADGCPYFAESRLTLICRKISFLDIDPHNFLDGTIAENYPKKDYHRMYV
                     GEIAKVLTRDGASGDAASSKS"
     gene            7843..8964
                     /locus_tag="PKH10_02005"
     CDS             7843..8964
                     /locus_tag="PKH10_02005"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNT26929.1"
                     /translation="MSYREELTSFAKNIRTLQKYLKSRDTVRQSLVVPFFKILGWDVY
                     NPGEFMAGYVFETPGEGAVAAADFAVLRDDRPAMIVQTLFGDEDTAEGFAMLSELFRR
                     IPGDKIAVLTNGIDWRFYADGMEQGVLDAEPYLTLNLGEKTRAEDLAVIDPYQKATFD
                     LAAILAPLRLGVYEKKIAEYFAAQRVASTADRELIEFIMKKVHEGEVSPKAREKLTVW
                     ILNAFAVIPPKTATTAPAPTPQPVQELEELEIVRPLSAPGKGGNGPAKKGGDANLMGP
                     EGQITVISGPDQGKTIPIHKKEFVVGRNLDLDLVLTDPSVSRRHFRIWFDNGAYWAED
                     LGSGNKIKVNGAKTTEKKRLANDDLIQAGNTTTRFEILH"
     gene            complement(9005..9181)
                     /locus_tag="PKH10_02010"
     CDS             complement(9005..9181)
                     /locus_tag="PKH10_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26930.1"
                     /translation="MRLLIVVMVMVACVAGVPFESRSGAAGAVVIAASPDPPPTPPDR
                     PNPPPPPPTSRTVV"
     gene            9327..>10121
                     /locus_tag="PKH10_02015"
     CDS             9327..>10121
                     /locus_tag="PKH10_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT26931.1"
                     /translation="MLRIVRCNDPFFSARHAPGRIAWSADEADRLIAGLIDTVRAGDP
                     VALPYAEHLFTTEARALRFLEAAFTAEAPVTFFCREMTLPSVLVRYALIEEEPENYQP
                     FAPHIAALPFSPAARETVTENISGFPYSRIEEILSAMRGLPEPEAIEYLLDTKRNLLR
                     RNPVIEVVKTGGGIEQIGGLAELKQWIVERRGNFSTKAREFGIPMPKGLLMLGVQGCG
                     KSLTAKTIANIWNFPLARLDFVNLFQQGKSVEELIRDAVAIAEAFAP"
BASE COUNT         1998 a         3335 c         2768 g         2020 t
ORIGIN      
        1 gccgctgaag gtattgttca tggtgtagtc gggcgcaccg gtcacaccac tcatcgcgat
       61 gccgaaagcg acgccgccgg caccgccgcc gccgccgccg ccgtgaccgc cacggcctcc
      121 tttgccacct ttgccacctg cgcccccaca gaaacgaccg gtaccaccat cgcctccgtt
      181 acctcccgca ccaccaacac caccgttgcc tccttttcct ccggccccgc cggctccgag
      241 gaagatggtg ttgttctgta gcgtcggggc cgtagtactg ataagaaaga gcgcaaaaga
      301 accaccaccg gccgtgccgt agttggcgcc ggtcgcacca cacccaccgg cacccccgcc
      361 gccgccatga ccaccgaggg tgtcatagta agcggtgccg cctgaatcgt caccgccgcc
      421 gccgccacca ccaccgccac caccaccacc tgcaccacca ctaccgccac caagaccgct
      481 tgcgccgatc cagttgcttc ctgatatccc accggtcgca ttgagcgcac cgctacctgg
      541 cacaccttca gtgccgcttg ccccattctc tccgttcaac ccttccatcg gcccatctaa
      601 cgcatagtca tcacagaccg tagatgtgcc cgatttgaaa aatctgccgt catatccttc
      661 ctctccgcca tcgcctccac cagatccttc acctgcgaag ccggttactc cgctgttttg
      721 ggtgttgtcg gagggaggac aggcattccc tccgccattg ccaccgttcg cattcgagca
      781 ggaagcattc gtgccccggt tagtgttctg acgcgaagca gtacagctat cggacgaagt
      841 ctcgtatgag ttcggcccgg aggtgccgga ggttgcgttc gaaaccaccg ttccggcagt
      901 tccgtcggta ccgggaccac ctgatgcacc gtatatcgtg ttgtctctga tatcgaggtc
      961 ggcatcgctc ccggtgatgt ggaacacata ggaattccct cccgccgcgg tcgccgcctg
     1021 accatatagc acgaaaccct ccaccaccgt cgaggaggtt atgcccgacg cgatgacggt
     1081 ccgtttatgg gtcgccgtat cgttgccccg ccagaccgta agcgatgatg cggtgtgccg
     1141 ctcccatgtg tcggccttgt agccaccgta gagcgatatg ccattcacga gcgtcaccgt
     1201 ctcttcgtag agtccgtcgg ccacatagac cttggtgcgg gtcgttgcct gagcccgact
     1261 gatcccgtac gctatcgttt tgcagggata atagccgaca acggtgccgc tcgggccgag
     1321 tccgcaggtg gtgtcgttgg tgccgttcga tgtcgagaca tagataacgc tcgtttcaag
     1381 aaggaactca cagccgtcat caggcacatt gttgaggttg taatagttaa cattgcaggt
     1441 caaccaacag accggactgc cggtcgcatt gcaggtgccg taaccgttgg gcttgttata
     1501 gatggcggtg cagtcggtcg aacagttgcc gcaggcggtg ttctggtcgt acttgccggt
     1561 cacctggttc ttgaaccctt cgtcgatctg tgtatcgcaa tcattatcaa gataatcgca
     1621 ctgcgtctcg gtcgtctcat attcgggacc gtagtttacc gccgtgcagg cgagccagcc
     1681 cgaaatccct ccacaggtct gccgcgatcc cttgcatacg ccgctctgaa gtggacaggc
     1741 cggcgcgtaa ctcgccacat tctcgtccgt ctcgtcatcg cagtcgttat tcttgccatc
     1801 gcagatcgtc tcgctctgct cataggtgcc attcgtctgg ttcgcatagt cggtcgggcc
     1861 gcaggggagc cagcctgaga agccaccgca cgactgccgg gtaccggcgc acaccccttt
     1921 ctggttcggg cacagaccgc cggtgaggcc atcgtccacg gcatcgttgc agtcgttgtc
     1981 cagaccgtcg cagtcctcgt cgtcggtcga cggcaacgct ttcacattgc actcggtatc
     2041 gcttccgtcc gttttgcaga catagatgcc cgtcacccga cacacaccgt acccgtcagt
     2101 gcagaccgtg ttcttttcat cgaacccgtt gtctatctcg ccgtcgcagt cgtcgtcgag
     2161 gttgttgcat ttttcccctt ccggatcggg caggcctttc tccgcgttac aaataacccc
     2221 ggtgcccggt tcgtcgcaga ccgtgaaacc cgaccgctcg cagatgccgt ccccttcagt
     2281 gcaggactta tagagcacct cctcgaacgg ttcatctatc tgatcattgc agtcattgtc
     2341 ggccagatcg caggtcgcct cgaccgtctc gtaccggctg tcccatgcaa ggtagtcgcc
     2401 cgtgccgcag gggagccaac ccgccatgcc accgcacgac tgacgggaac cgaaacagac
     2461 accctgctgt ttcagacaga acggcgcgga tgattgcaga ttatcatccg cggtgccgct
     2521 acaatcgtcg tcgatgccat tgcaaacttc ggtcatgccg gggcgcacat ccggatcggt
     2581 ctcgtcgcag tcctgcaact gacacatcgc accgacgccg gccatgtcgc cgtcgaaatc
     2641 gacacagcag atctggctgt cgggcttgca tccgcgaccg aggaagttgt cctcctcgtc
     2701 gtagatatcg agacaggaga acgacggcag gcactcttcg tcgctcacgc aggcggtcag
     2761 acacaccgat gtaccaagta gcgacgcgca aatatcggtc gaataggcgc acggcaggcc
     2821 gccggcgcac gaacgacata accacccgtc ggcgatcacg cacccgtcgg tcgggttgca
     2881 catctccgcc gcatcgcaca gttcgtgacg cggcaggtgg aggcactggt cgttcaggac
     2941 gacctcttcg gtgcagtcct gcccgtcatc ctcacaggtc accagttcgt ccgtatcggt
     3001 caggaggata tcatcatcac ccgtgaaggc gtcgaactga tcggggaaga tatcgttggt
     3061 gtcaggcgtc ccgtcatcaa agggcaaagt gtcgtcttcc gactctacga catccgggac
     3121 cgttatgatg tcctcgtcgg tcatatcgtc aaacattgcc tcgtcaccac cgtcccaggc
     3181 ctcatcgacc atctcgtcgg gaatgacctc atcggggtac gcttcgtcct catccggttg
     3241 cgtatcgcct tcaggcatcg tgtcgctgtc atccggctgg gcactgtctc cgtccagcgg
     3301 agcatcgtcg gtcgccgtgg tgtcggtctc gggaagcgtc ttatcgctct ccggcgtcag
     3361 ttcgtcgttc cctccattat cgtcgaccga cggcttggag gagccgtcgc aggcggtgag
     3421 gaagagaagg aacagggcga taagcatcgt cgcgttcttt ttcatagccg cctccctaat
     3481 aaccgcgagt tgtgtacacg gccccgatgg atcccgcgga tccgggtagc cccatcgaag
     3541 aaccccccgc gccaccggca ccacccttgt caggtgtgtt gccgtaatag gtgttcccgg
     3601 tagcgaagtt cggagccgta ccgctgtagc cgataccgac ggccgtcccg ccgcatcccc
     3661 ctccgccacc gccgcctgca ccaccgttgc ccccgttacc accggcgcca ccaaccccac
     3721 cacagttgag tggcggagta ttaagcgtgt tgcctcccgc tccaccaatg cctccctctc
     3781 caccggaacc gccggtaccc ccattaccac cacggccacc actgccaagt gttatcgcat
     3841 tgtcacgcaa tactaccgca ccggtgcttc cggtcacgaa tatcgcgaaa ctaccaccgc
     3901 ccgcggcccc gccgctggcg ccggtgccac cacagccacc tgcgccaccg ccaccgccat
     3961 gagagccgaa cacatcattc tcgcccgata tgtcgctgtc gcctccgccg cccgatccgc
     4021 caccgccacc accgccaccg gctgtaccaa caccaccagc attcccgcca gcaccgcccc
     4081 agtgaccgga aacaacagat ccgttcgttg acgagcaccc ggtgccaccg gagccgtccg
     4141 tcccgctccc accattcgtt ccgttcgtcc cgttcatgct tcctgatggg atcgtgcagt
     4201 ccatgttata gggaggattg taagtcactg tatggacacg accatcatag ccacggccgc
     4261 cgccgactcc ggcgccggaa ccacttccgt tgtccccgac acgggaactt ccatagttat
     4321 tgtaactagg ggcacaatag ttgccgccgc ctctgccacc ggcaacacca caggaactag
     4381 atccaccatt attgctctga cgcgtgatgg tacacccaga atcacgcccg ttcgcggaaa
     4441 ccggatcgta tcccgcaccg tcagcgggtc tgccaccacc gtcctcaccc ggcgcgccgg
     4501 cattcgcctg ccccatgctt gcgccgggac cacccatcgc accgtagatg acattgttgc
     4561 ttatcgtcaa actagcattc gaaccggtca catagatgac atacgagttg ccgccagtcc
     4621 cggtcgcgct ctgaccgtac aagacaaagc cgtcgagaac agtcgttgcg gtgatggcac
     4681 tggcgatgat ggtcgctttg tgcggttcgt aatcaaatgt attgcctcgg atgatggtca
     4741 tcgtggtcgg cgcatgcctt ccccaggtat cgggacggta accgccgtaa agactcttgc
     4801 cgggggtcag tatcactgtc tcctcgtagg taccgtcggc cacaaatatt ttgctccgat
     4861 tcagtggcgc tccggcccgt tgaagcccct ttgcgatggt cgcgcacggc ttctgaccaa
     4921 tcgccacggg tccgagacca cagttcgggt caagatcgtc accattcgac tcggagacat
     4981 agatcgcgtc gacatcgagc gtgaactcgc acccgtcgtc gggcaccccg ttaaggtcga
     5041 aatagagcga ggtgttggta cagcgcatct tacattccgg tgttccggtc gcgtcgcacc
     5101 acccctcggc gttaggtttc gcgtaaatgg tcgtgcaatt ggtcgcgcaa ttgccgcacg
     5161 cggtattctg gtcgtatttg ccggtccccg cgttcaggaa gccttcgtcc agctcgccgt
     5221 cgcagtcgtt gtctaggtaa tcgcaccggt tctcggtcgc ctgatagtca gcgccgaact
     5281 gagccgcacc acaggcaagc caactcgaag cgccaccgca cgccttccgg gcgccgacac
     5341 aaacaccgcg caacggttgc gccgggccga ccgggttgtc acaggcagga gcatagttcg
     5401 tcaggttgtt gtcgaccccg ccgatgcagt cgttgtccag cccgtcgcac agcgtctcgc
     5461 tgacctcgta ggtgccgccg gaggccgcca gatagtcgcc gtatccgcaa tcgagccaac
     5521 tagcggtgcc gccgcatttc ttccgggcgg tcgaacagac gccggcggtc agcgcgcact
     5581 gcggcgcgct ggcctcgacc ccgttgtcta tcccgagcac acagtcatcg tcgatgccgt
     5641 tgcactcttc atcggtcggg gtaccctgcg tcacactgca gtcgagcccg tcgccgtccc
     5701 cgttgcagat cttgatgcct gcccgcaggc aggctcccgt cccgacaaag catacctccc
     5761 ccttctgctc gaatctgctg tcgttatcga tgaaggtgtc gcaatcgtca tccttgttgt
     5821 tgcacagttc ctcggacgcc ggatccgcct tgaccgcgtt gcataccact ccgtcgtgcg
     5881 tatcgttaca caccatgaac ccggcccgtt tgcactcacc gataccgtcg gtgcagatct
     5941 caaaaagctc cgtggcgaac ggatcgtccg tcacgccgtt gcaatcattg tcgaccccgt
     6001 cgcagagagc ctcggtctcc tgatagagat cgttccacgc gaggtactgc gccgtcgtgc
     6061 aggcaagcca tcccgccgtg ccaccgcagg tcttgcgaag tccctgacag atacctccct
     6121 gtttcgcgca taacggcgcg gtgccaaggt tattgtcgac gataaggtcg caatcgtcat
     6181 ccgccccgtt gcattgttcg atcgcgccgg gatagcgctg agcgctcgat tcgtcgcagt
     6241 cgtacagagc gcatttggca ccgacaccgg cattatcacc gtcgatatct agacagcaga
     6301 cgaaattcgt cggcgcgcat ccacgaccca acaaaagccc ctgcaggtca aagaggtcgt
     6361 tgcatcggaa atcaccgggg cactcggcat cggtatcgca gggaaggaga cagagtttct
     6421 cgcccaaaag cggggcgcag atgtcgccgt catacgggca gggagcgctc tcacaggaac
     6481 gacagaccca ctcgtcgggc tcaacgcatc cgtcggtctt gttgcagagc agaccgggct
     6541 cgcaccattc gtggcgcggc aggtgcttgc actgaccgcc gataatgaga tcctcggtgc
     6601 aggttatctg gtcgttgcat ccgaccggcg aatcgatgtc ggggagcacc cacgcgtcat
     6661 cggccacgaa ctcgtcctcg acgaccgcct cgtccgggta ttcctcgtcg gggtcatcct
     6721 cctcctgctc ctcgtcaacg atcggctcgt cgatatcggt cgcctcgtca acgaacaatt
     6781 catcatcgtc aaagatatct gcaccgtcgt caacgggtac cgcgtcgtcc ccgaccgtcg
     6841 tatcaccatc aggggccgac ccctccgtgt cgggcgtcac atcggtctcc tcgtcagtcg
     6901 gcaggatctc atcggtcagt gcatcccgat cggcaagcag gctatcgccg tctcccgtcg
     6961 tatcttcgtc cttcgaactt tgcggcttgc tcgaggaaca ggcgagcacg gtcaggagca
     7021 acagggaaaa cccgcaacgc aacaggtcaa gaagaaagcg cctcatcggt atcctcccgg
     7081 agagaacttc ataccttatt tatttaacac aacgacgatt ataaacggtc gaaagcaaaa
     7141 cgcaagggtc acgcgcacat tccggccgct ctcttttttg acaaaccgtt ccattcccgg
     7201 taccatgcgt tctgcacgac gaccaacaag cggagaccga catgggacag gagaattttc
     7261 gcgagatcac accggccgag atgaccgaca atcctttcac cgctttcggt aagggatggg
     7321 cgctcctcgc cgccgggaaa gggaaaaaaa gcaacatgat gaccatcgca tggggcgcgt
     7381 taggcgttct ctggcaccgc gaggtctgtt tcgtattcgt ccggccgccg cgccacacct
     7441 atcagcttac cgaggccgag ccttgctttt ctgtgggttt tttccccgag cagtaccgcg
     7501 atgtcctgca gttgtgcggc tcgatctccg gcgccaagga ggacaagata gccgcctcga
     7561 aactgacgct cctcgatgcc gacggctgtc cctatttcgc cgagtcgcga ctcacgctca
     7621 tctgccgtaa gatctcgttc ctggacatag accctcacaa tttcctcgac ggaacgatag
     7681 cggagaacta ccccaaaaag gattatcacc gtatgtatgt gggagagatc gccaaggtgc
     7741 tgacccggga cggtgcctcc ggagacgcgg cctcctcaaa aagttgattt agtttttccg
     7801 agcgattaga atcgaatggt caataaccta ccggaggcga tcatgtcgta cagagaggaa
     7861 cttacctcgt tcgcgaagaa catccgaacg ctccagaagt acctgaagag tcgcgacacg
     7921 gtgcgccagt cgctcgtcgt cccgttcttc aagatcctcg gctgggatgt ctacaatccc
     7981 ggggaattca tggcgggata tgtgttcgag accccggggg agggagcggt ggccgcggcc
     8041 gacttcgcgg tgttgcgcga tgaccgcccg gcgatgatag ttcagaccct cttcggcgac
     8101 gaggacacgg cggagggctt tgcgatgctc tccgaactgt tccgtcgcat accgggcgat
     8161 aagatagcag tactcaccaa cggcatcgac tggcgttttt acgccgacgg gatggaacag
     8221 ggcgttctcg acgccgagcc ctacctgacg ctgaatctcg gcgagaagac ccgtgccgag
     8281 gatctcgcgg tcatcgaccc gtaccagaag gcgaccttcg acctcgcggc gatactcgcg
     8341 ccgttacggc tcggcgtgta cgaaaagaag atcgcggaat acttcgcggc ccagcgggtc
     8401 gcctcgacgg ccgacaggga actcatcgag ttcatcatga agaaggtcca cgagggagag
     8461 gtttcgccga aggcccggga aaaactcacc gtgtggatac tgaacgcctt cgcggtgata
     8521 ccccctaaaa ccgcaacgac cgccccggca cccactcctc aaccggtgca ggaacttgag
     8581 gagttggaga tcgtccgtcc actttcggct ccgggcaagg gtgggaacgg tcccgcgaaa
     8641 aaagggggcg acgccaacct gatgggaccg gagggacaga taacggtcat atcgggaccc
     8701 gatcagggca agacgatacc gatacacaaa aaagaattcg tcgtgggccg taacctcgat
     8761 ctcgacctcg tgctgaccga cccgtcggtc tcacgccgtc acttcaggat atggtttgac
     8821 aacggcgcct actgggccga ggacctcggg agcggcaaca agataaaggt gaacggcgcg
     8881 aaaacgaccg aaaaaaagcg tctcgccaat gatgacctca tacaggcggg taacaccacg
     8941 acccgtttcg agatacttca ctgaaccgaa cggaccctca aaaaattgta agagaatatc
     9001 tcggtcagac gaccgtacgg ctggtcggcg gcggcggcgg cgggttcggg cggtcgggcg
     9061 gggtcggcgg cggatcggga ctggccgcga tcacgaccgc accggcggca ccggaccgag
     9121 actcaaaggg aacgcccgcc acacaggcga ccattaccat gaccacgata agcaacctca
     9181 tgggcacaat ctcctcatac cttgacctct actgtcccta ttctagtgaa ttgaaaaaaa
     9241 gatgtcaata ggtccgccac attattttga ccccgtcgat ttttggcata taatccgttt
     9301 taccatcaga cgaggggaga gacgccatgc ttcgtatcgt ccgctgtaac gacccgttct
     9361 tcagcgcccg tcatgcaccg ggccgtatcg cgtggagcgc cgacgaggcc gatcgcctga
     9421 tcgcgggcct catcgacacc gtgcgggcag gcgatccggt cgcacttccc tacgccgaac
     9481 acctttttac cacggaagca cgggcactcc gtttccttga ggcggccttc acggcggaag
     9541 ccccggtcac tttcttctgc cgcgaaatga ccctcccttc ggtgctcgta cggtacgctc
     9601 tcatcgagga agagcccgag aattaccaac ccttcgcccc ccatatcgct gctctgcctt
     9661 tttccccggc ggcgcgggag accgtcaccg agaatatcag cggcttcccc tactcccgga
     9721 tcgaggagat actttccgcc atgagaggcc tgccggaacc ggaggcgatc gagtacctgc
     9781 tcgataccaa acgcaatctt ctgcggcgca acccggtcat cgaggtggtc aagaccggtg
     9841 gcggcatcga acagatcggc ggtctggccg agctcaagca gtggatcgtg gaacggcgcg
     9901 gcaatttttc gacgaaggcg cgtgaattcg gcataccgat gcccaaaggt ctcttgatgc
     9961 tcggcgtaca gggatgcggc aagagtctca ccgccaagac catcgcgaat atctggaatt
    10021 tccccctcgc gcgactcgat ttcgtgaatc tgttccaaca gggcaagagt gtcgaggaac
    10081 tcatccgcga cgccgtcgcc atcgccgagg cgttcgcccc c
//
LOCUS       DAOQUE010000036        10049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_7513_length_10049_cov_6.923054, whole genome shotgun sequence.
ACCESSION   DAOQUE010000036 DAOQUE010000000
VERSION     DAOQUE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7513_length_10049_cov_6.923054"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKH10_02020"
     CDS             complement(<1..209)
                     /locus_tag="PKH10_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNT26932.1"
                     /translation="MKNAQRDRVLLSVALNTGLRIGDILRLTVGEVTPDKPHTFITQQ
                     QKTPKDEPQPRRRPPPGPRRRSRRPR"
     gene            447..776
                     /locus_tag="PKH10_02025"
     CDS             447..776
                     /locus_tag="PKH10_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNT26933.1"
                     /translation="MTNRKTVIDACTLLNRCELLLLRKARPFLPGEYSILAALEEQGG
                     RRSSDIAELFSFLPSKASRYIKNLEERALIRRELGKKDLREFFFYLTPKGREKLKELD
                     ALIKQLT"
     gene            complement(831..1331)
                     /locus_tag="PKH10_02030"
     CDS             complement(831..1331)
                     /locus_tag="PKH10_02030"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT26934.1"
                     /translation="MTKYIMLLTPLFLLLACGGDDGNDPVDDNAQSDADTTPVWVTYA
                     GIEWSPLRTRRVTWESAKNYCGAMGARLPTINELRKIIINCPQTEYGGVCKASDPDCL
                     RWDCWSEGCDCDGSADSYSALGDIHRLWSSSSRVEIYAAWFVDFSDGKVSYYNKSGEQ
                     NTRCVR"
     gene            complement(1828..2097)
                     /locus_tag="PKH10_02035"
     CDS             complement(1828..2097)
                     /locus_tag="PKH10_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26935.1"
                     /translation="MNLTHHGNTRSLQRNLADALHRVLDEGLADLATTYHHHGTRYLF
                     FGKRFGSIEGLVLVTDPRSDTIITAYRNRDFPRWIKSRIRKKNLH"
     gene            complement(2161..5349)
                     /locus_tag="PKH10_02040"
     CDS             complement(2161..5349)
                     /locus_tag="PKH10_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNT26936.1"
                     /translation="MYQDQTLLLSDDERRRLNEVILDQLLYGKNPEGRAPYTPEEVFN
                     RYTGKGGLHGLAYKDYSSRHAYAAAKREYEQGQFFTPHWVCRSIVETIRPHPSDKVAD
                     LTCGMGNFANWLPDQKNFYGCDIDPDAVAVAKFLYPDADIEEQDIRWMTRLRPEYDLI
                     LLNPPYALRFEVYDPVERAYMTEASEFVCVKKAADLLKPAGYLVLIVPETFLEDEYSN
                     RTKLDIVNDAFRFLCQYRLPKDAFAGVGVETFPTKVMLFQRRGAALSEKPYTPGVFVE
                     PASVFPDHLAAAYAEKSKIKYLVGKDLWARSAEDRRFQDKVTKLLFDIKRHRKTAEYY
                     PKAAALVKEYQTQKRPTGMTDAEWEKVRLTPNKVLYRLKKAVAYQSRIERDEIRLVKT
                     SYGLKLKAYSAKSKAALGKLKVEKRASFNDMLVQKAYPFNDKAYYRLFLRKQREYERQ
                     AESFKTMGPDPILAQWLAEFRLYDKALYRAPRPASDTDTATLTTTPAPVAYQPPPAQR
                     EKPFQLSLFSSPALYAQAPQPGELILTDIQQEDLGKVLRKRYALLAWEMGCGKSIAAI
                     AWLKYLYAKERLNYSFIVGPAVAIKLTWVKLMRKYGLDHIQIQSEKDLERIRPGQFVL
                     LPLSIIGRLERRLLHFFKLQVKRSRTALVLDESDECANYRSGRTVSTLRLFKRLGYKL
                     LTTGTPTRNNVNEIYPQLELLYNNSINLLCECDTIYKLDRETKEIAPHPNDHYYLKPF
                     PPFRGQTLFNRCFNPEKTSVFGVKKKSQDILQVEELIRLLDKSRITRTFEEVVGEKKY
                     KVFTHKVWQSEPERELYRIIMEEFVRMMKYFKQSEDSRKESLLKIIRQILLLIKAGSI
                     PHVMQEYSCTQEPEKAKKILELVGSWPNEKVAVGTIFKEAAAYYARKLREAFPDRELF
                     VIEGSVEFGKRSKIIEEFQATPNGILVSTQESLKSSVNIPTCNRCILECLNWNLSKMS
                     QYYFRFIRFDSKDPKEVHMVVYEDTIEMNILALLLAKEKMNEFVKTSSTVSNEELFGE
                     YDIDSSILDSILEKATNEDGESYLRWGRQKAA"
     gene            complement(5914..6552)
                     /locus_tag="PKH10_02045"
     CDS             complement(5914..6552)
                     /locus_tag="PKH10_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26937.1"
                     /translation="MKMGSTIVKKGKEYYLIIPQEILRKLNCDTFDMRVDKEKKELVL
                     TPAKSKKGKDASSGKKKKMSKAEEKQLLADLRSGKVGMEDDPEEEAALIAAFERGEFK
                     PISAARKRQLMKDAAATLAAMKKKPLLPMTYKGYLGTYKHDKKANIFHGEVIGIEDVI
                     TFQADAEKDMEQAFRDSVDDYLLFLKALKRSNKEREDIPYEKAVKQIKKLRS"
     gene            complement(6573..7526)
                     /locus_tag="PKH10_02050"
     CDS             complement(6573..7526)
                     /locus_tag="PKH10_02050"
                     /inference="COORDINATES: protein motif:HMM:NF023701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HNT26938.1"
                     /translation="MQQNAQLDKDLYPVLKELQDRKALEHLVLIGGQAFNYYSLHYGS
                     SALESKLTTDADFFVPHPHRKISVAADLSKIFLSLGFKEYKTDWHHRVLSYRKGDFDI
                     EFIYPAFKDRHGRTEPIPRLGINAVALNYHQVYSKYRMEVTYREVRFFVPEPEAFLFV
                     KYITSQQRRDIRKKMKDLTTARSMSEFVYASEDRLNRLVVILNDTEVIPYAWKKKFFG
                     IMEKQVPDFHKKVLLLDGEYAAKQYGEKIGRTFNTKELRALAQAEAALRKGNFERFRE
                     RIYEAVLDGKDVSRMTLFVNRELTNQRRAKAKKTGKEKGTP"
     gene            complement(7504..7923)
                     /locus_tag="PKH10_02055"
     CDS             complement(7504..7923)
                     /locus_tag="PKH10_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26939.1"
                     /translation="MRYAALTWMMIQYDQWTDSLEAARRFVAGASKGYLSRKLKNGRG
                     YWYLQYREGSRILSQYVGTDDEKKVKETATLIEKRREEERYIRRMERELHVLRKAIRA
                     IIARIAKRISRRKDRTMSEYEKHILTRWSRHATERAA"
     gene            complement(7920..8120)
                     /locus_tag="PKH10_02060"
     CDS             complement(7920..8120)
                     /locus_tag="PKH10_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26940.1"
                     /translation="MFEQLKNEHAREQEQIELLSRRLANGDIADSGFASLRALKQRLR
                     ELKEDVAILSIAIRVYERCGQS"
     gene            complement(8264..8494)
                     /locus_tag="PKH10_02065"
     CDS             complement(8264..8494)
                     /locus_tag="PKH10_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26941.1"
                     /translation="MEQNPKATKVILTGPEKVPSVKKPGTDIETFKVTILTEGLNALK
                     TYICHSFEKAATVAADIARDRGLELVDQTVPF"
     gene            complement(8572..8988)
                     /locus_tag="PKH10_02070"
     CDS             complement(8572..8988)
                     /locus_tag="PKH10_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26942.1"
                     /translation="MSYRYRTLAHSIKEGNGIPSAAFLIWDRIREESSPVFIPMPSHT
                     GKPSSWMRDLCWYLARLSGGTVIDALRGNRRMSLYLAKKQRKKVTVKMKQVHPVPANA
                     FIVDNVIATGLTMRTAQTAIAVPTRPLALTVASRHP"
     gene            complement(9314..9769)
                     /locus_tag="PKH10_02075"
     CDS             complement(9314..9769)
                     /locus_tag="PKH10_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HNT26943.1"
                     /translation="MKKEYFRMTKKQLLSLLASSNLIGEKRPNVKNSEDAVKTAATVI
                     TNWQQENFVVIYLDAAGHPIDTRVIFIGTLNQSLVHPRETFAPAIELRAASVILAHNH
                     PAGTPDPSAEDLAVTKRLIESGKILGIEVLDHVIITVDSHYSFRQSGLI"
BASE COUNT         1935 a         2585 c         3024 g         2505 t
ORIGIN      
        1 cgaggtcggc gagaccttcg tcgaggaccc ggtggagggc gtcggcgagg ttgcggctcg
       61 tcttttgggg ttttctgctg ttgtgtgatg aaggtgtggg gcttgtcggg ggtcacctcg
      121 cccacggtga gccgcaggat gtcgccgatg cgcaggccgg tattgagggc gaccgaaagg
      181 aggacgcggt cgcgctgggc gtttttcatg gtcttggccc tcgcagatag acacaacatc
      241 aacatccgat atgtcgcctg tttttcatcg ggcatcagga taatttccga caaaaagagt
      301 aaatataggt ggttgcaccg ataacacaac acaatgtgta atgtgttgta tcgagcaccg
      361 gaaaagtgat ggactagaat aaattgacat tgaaagatac ctttcacagt atcctctctg
      421 tcgaagcagg aaaggagacc gacgacatga cgaacagaaa aacggtgatc gatgcttgta
      481 ccttactgaa ccgctgtgaa ttattgctgt taagaaaagc gcggcccttt cttccggggg
      541 aatacagcat actcgcggcg ttagaggaac aagggggcag aagaagttcc gatatcgcgg
      601 aattattctc tttccttcct tccaaagcaa gccgttatat caagaatctt gaagaacggg
      661 cgctcatcag acgggaactg gggaagaaag acctgcgcga attctttttt taccttaccc
      721 ctaaagggcg cgaaaaactg aaagaactcg atgcccttat caagcagttg acctgatcgc
      781 gcgggatacc gccgttgtag ggtgagccgc cgcaagcctg aaaggaaaag tcatctcaca
      841 caacgggtat tttgctcgcc gcttttattg tagtaggaaa ccttgccgtc gctgaagtcg
      901 acaaaccatg ctgcgtagat ctccacacgg gaggaagatg accagagtct atggatatct
      961 cccaatgcag agtatgaatc agcagagccg tcacaatcgc aaccttcact ccaacaatcc
     1021 caacgcaagc aatcgggatc ggaggcctta cagaccccgc cgtattccgt ttgtgggcag
     1081 ttgatgatga tcttccgcag ttcattgatg gtaggcaatc gggcgcccat tgctccgcag
     1141 tagttcttag ccgactccca tgtaaccctt cttgttcgca gtggcgacca ttcgatgccg
     1201 gcgtaggtga cccatacggg ggttgtgtca gcatcggatt gggcgttatc atccaccgga
     1261 tcgttgccgt catccccgcc gcaggcgagc agcaggaaca ggggcgttag gagcatgata
     1321 tatttggtca tgggtcccct cccttgaatg aaattgaacg gtgtattata tccgactgaa
     1381 aggaggaggc aaatttctcc agttcccttc cccgcccgcc ttcaaccgag ccgctgacca
     1441 cggatagccc ctttaggggc gtaggccgag gacttgtttg aggcgccgaa gcgtatcccg
     1501 cgaaggtggc gagttccgca ggccccgtgg tcgaggcgat ccggcaacgc cggcggttgt
     1561 cccggcgggc attaaccggt ttcttctttc ccctctttct tctttgccgg accaaagacc
     1621 gaaggacgaa aagaaaaggc tccggtggag cgttttcagt ccgaaggccg caggcttcag
     1681 ccgaggcatg ggggtcggcg tagcacgcgc agcgacgccg aaactctcga cccccccgaa
     1741 ggaccattgc cgcccggacc tccgctgtgt cactcggtgg aggaggggct tgcggggcat
     1801 cgccgttaag caacgaccgg gcggcgttca atggaggttc ttcttcctga tccgtgactt
     1861 gatccaacgg ggaaagtcgc ggttccggta ggccgtgatg atagtatcgc tccggggatc
     1921 ggtaacgagc accagtccct cgatactacc gaaacgcttt ccgaagaaga ggtaacgggt
     1981 cccgtggtgg tggtaggtgg tggcgaggtc ggcgagacct tcgtcgagga cccggtggag
     2041 ggcgtcggcg aggttgcgct gtaaagagcg cgtgttccca tgatgagtca aattcatcgg
     2101 agtctcctgt ttgttaatgg ttgtaattgg gggtttgtcc ccgatcccgt gtcgcgagtc
     2161 tcaggccgcc ttctggcggc cccagcggag ataactctct ccgtcctcat tggtcgcctt
     2221 ctccagaatc gagtcgagta tcgacgagtc gatgtcatac tcgccgaaga gttcctcgtt
     2281 ggaaacggtg gaggaggtct tgacgaactc gttcattttc tccttcgcga ggaggagggc
     2341 gagtatgttc atctcgatgg tgtcctcgta gacgaccatg tgcacctcct tcgggtcctt
     2401 ggagtcgaac cggatgaacc ggaagtagta ttggctcatc ttcgacaggt tccagttgag
     2461 gcattcgagg atacagcggt tacaggtcgg tatgttcacg gaggacttga gcgattcctg
     2521 cgtggagacg aggatgccgt tcggggtcgc ctgaaattcc tcgatgatct tggatcgttt
     2581 cccgaattcg accgagcctt cgataacgaa tagttcccgg tccgggaacg cttcccgcag
     2641 tttccgggcg tagtaggcgg cggcctcctt gaatatggtg ccgaccgcta ccttctcgtt
     2701 gggccatgag ccgacgagtt cgagtatctt cttcgccttt tccggctcct gcgtgcagga
     2761 gtattcctgc atgacatgag gaatggagcc cgccttaatg aggaggagga tctgccggat
     2821 tatcttcagg agcgactctt tccggctgtc ttccgattgc ttgaagtatt tcatcatccg
     2881 gacgaattcc tccatgatga tgcggtaaag ttcccgttcc ggttccgatt gccagacctt
     2941 gtgggtgaaa accttgtact tcttctcgcc gaccacctct tcgaaggtgc gggtgatccg
     3001 gctcttatcg aggagccgta tcagttcttc aacctgcagg atgtcctgcg atttcttctt
     3061 gaccccgaac acggaggtct tctcggggtt aaagcaccgg ttgaagaggg tctggccccg
     3121 gaagggaggg aacggcttca gataatagtg gtcgttcgga tgcggtgcga tctctttggt
     3181 ttcgcggtcg agtttgtaga tggtgtcaca ttcacagagg aggttaatgg aattattata
     3241 gaggagttca agttgcggat agatctcgtt gacattattg cgggtagggg ttcccgtcgt
     3301 caggagttta tacccgagcc gcttgaagag ccgcagggtc gaaacggtgc gacccgaacg
     3361 gtagttcgcg cattcatccg attcatcgag gaccagcgcg gtccggctcc gtttcacctg
     3421 caatttgaag aaatgcagga ggcggcgttc gagccgcccg ataatggaga ggggcaaaag
     3481 gacgaattgc cccggccgga tccgttcgag gtctttctcc gactgtatct ggatgtggtc
     3541 caagccgtat ttccgcatga gtttcaccca agtcagtttg atggcgaccg caggaccgac
     3601 aatgaaagag tagttgagac gctctttcgc gtagaggtac ttgagccacg cgatcgccgc
     3661 tatcgacttg ccgcacccca tttcccatgc gaggagcgcg taacgcttgc ggagtacctt
     3721 cccgagatct tcctgttgga tgtcggtcag aatgagttct cccggttgag gcgcctgcgc
     3781 atagagtgcg ggcgacgaga agagggaaag ttggaacggt ttttcccgtt gcgccggagg
     3841 cggctgatag gcgaccggcg ccggcgtggt agtgagggtg gcggtatcgg tatcggaggc
     3901 gggacggggc gcacggtaga gcgccttatc gtagagccgg aattcagcga gccattgggc
     3961 gaggatcgga tcgggtccca tcgtcttgaa actttccgcc tgacgctcgt actcgcgctg
     4021 tttccgcaga aaaagtcggt agtaggcctt atcgttgaac gggtaggcct tttgtacgag
     4081 catatcgttg aaggaagccc gtttctcgac cttaagtttt ccgagggccg ccttcgactt
     4141 ggccgagtag gccttgagtt tcaggccgta ggaggtcttg accagccgga tctcgtcgcg
     4201 ttcgatccgc gattggtacg cgaccgcctt cttgagccgg tagaggacct tgttcggggt
     4261 tagccggacc ttttcccatt cggcgtcggt catgcccgtc ggccgtttct gggtctggta
     4321 ttctttcacc agcgcggcgg ctttcggata gtattcggcg gttttccggt gccgcttgat
     4381 gtcgaagagg agtttcgtca ctttatcctg aaagcggcgg tcttcggccg atcgcgccca
     4441 gaggtcttta ccgaccagat acttgatctt gcttttctcg gcataggcgg cggcaagatg
     4501 atcggggaac accgaggccg gctcgacgaa tacgcccggc gtatagggct tctcggagag
     4561 cgccgcgccg cgccgttgga ataacatgac cttcgtgggg aaggtttcga cgccgacccc
     4621 cgcgaaggcg tccttcggga gtcggtactg gcaaaggaaa cggaaggcgt cgttcacgat
     4681 gtcgagtttc gtccgattcg agtattcgtc ctcaaggaag gtctcgggga cgatgaggac
     4741 aaggtaaccg gccggcttga ggaggtcggc ggccttcttg acacagacga actccgaggc
     4801 ctccgtcata tatgcccgtt ctacagggtc gtatacctcg aaccggaggg catacggggg
     4861 attgaggagg ataaggtcgt actccggccg gagtcgggtc atccagcgga tgtcctgctc
     4921 ttctatgtcg gcgtcgggat aaaggaactt ggcgacggcg accgcgtccg gatcgatatc
     4981 gcaaccgtag aagttcttct ggtcgggaag ccagttcgcg aagttcccca tgccgcaggt
     5041 aaggtcggcc accttatcgg aggggtgggg gcgtatcgtc tcgacgatgg aacggcatac
     5101 ccagtggggc gtgaagaact ggccttgttc gtattcccgt ttcgccgccg cgtaggcatg
     5161 acggctggaa tagtccttat atgccaggcc gtggaggccg cctttcccgg tgtagcggtt
     5221 gaaaacctct tccggtgtgt agggagcccg cccctcgggg ttcttcccgt agaggagttg
     5281 gtcgaggata acttcgttca gacgacgccg ttcgtcgtcg gagaggagta gggtttggtc
     5341 ttggtacatg agggcctcct taaatgcgcg cctcgtaatc gggcgcaata ctgtgttgct
     5401 gcctccctcg ctctcctcga cttacgagag taagccttcg tcggctcggt cgtcgcgccg
     5461 ggtcttgcgt tccgacccga ggcgcttaat gcgaagagag cggggattat ttgtctgttt
     5521 tcatattcga tcagcccgcc ttcaaccgag gggcatcaag aggccatcgg tggttattct
     5581 aacgcgagca cctgtttgac ccgccgaagc gtactcgcgg aggtggggag ttgcgcagcg
     5641 tcctcttgat gcaccggtac ggcgtagccg gcggttgagt cggcggggaa gaaaaaggat
     5701 ttcttctttc ccctctttct tcttcacccg aaagaagaaa gggggtcgcc gtagcacgcg
     5761 cagcgacggc gaaactccct cgttcctctt tgggttcagc cgcccggtcc cggccgtgtt
     5821 actggcaggg aagggggcgg cgataacatt attggttgat gatgtggttg tagcgcttca
     5881 aagaagcgcg cgttgatcag aactgttgga acactaggat cggagtttct tgatctgctt
     5941 taccgctttc tcgtaaggaa tgtcctcgcg ttccttgttc gacctcttga gggctttgag
     6001 aaaaagaagg taatcgtcga cggagtcgcg gaacgcctgt tccatatcct tttcggcgtc
     6061 agcctgaaag gtgatgacat cttcaatgcc gatcacttcg ccgtggaaga tattggcctt
     6121 cttatcatgt ttgtaggtgc cgagataacc cttgtaggtc atcgggagta ggggtttctt
     6181 tttcattgcc gccaaggtcg ccgcagcgtc tttcatgagt tggcgtttgc gcgccgccga
     6241 gatcggtttg aattcgcctc tctcaaaggc cgcgatgagt gccgcctctt cttccggatc
     6301 gtcttccatg cctactttcc ccgaacgaag gtcggcgagc agttgttttt cttcggcttt
     6361 ggacattttt ttcttcttgc cggaggatgc gtctttccct tttttgcttt tcgccggcgt
     6421 aaggaccaac tctttttttt ctttatcaac acgcatgtcg aaggtatcgc agttcagttt
     6481 gcgaaggatc tcttgcggta tgatgagata atattctttg ccttttttga cgatggtgga
     6541 tcccattttc atagcagact tttttaactt tttcatggtg ttcctttctc cttgccggtc
     6601 ttcttggctt tcgcccggcg ctggttggtg agttcacggt tcacgaagag ggtcattcgc
     6661 gaaacatctt ttccgtcaag gaccgcttcg tatatccgtt cccggaaccg ttcgaagtta
     6721 cctttacgaa gagccgcctc ggcctgcgcc agtgcgcgaa gttcctttgt gttgaaggtc
     6781 ctgccgatct tttcgccata ctgtttcgcg gcgtattcgc catccagaag caagaccttc
     6841 ttatggaaat cggggacctg cttctccatg atgccgaaga acttcttctt ccacgcatag
     6901 gggatgacct cggtgtcgtt aagtattacg acgagacgat tcagccgatc ttcggaggca
     6961 tagacaaact ccgacatcga ccgtgcggtc gtaaggtctt tcatcttctt tcggatatca
     7021 cggcgctgtt ggctcgtgat gtatttcacg aagaggaacg cctccggttc cgggacgaag
     7081 aaccggacct cgcggtaagt gacctccatc cggtacttgc tgtagacctg atgataattc
     7141 agcgccaccg cgttgatacc gagtcgcggt atcggttcgg tgcgaccgtg ccggtccttg
     7201 aaggcggggt agatgaactc tatgtcgaaa tcgcctttcc ggtaggagag cacccggtgg
     7261 tgccaatcgg tcttgtattc cttgaacccg agagaaagga atatcttcga aaggtctgcc
     7321 gcgaccgata tcttacggtg cggatgggga acaaagaaat cggcatcggt ggtcagcttc
     7381 gattcgagag ccgaactccc gtaatgaagg ctgtaatagt tgaacgcctg tccgccgatg
     7441 aggacgaggt gttcgagtgc cttccggtct tgaagttcct tgaggaccgg atagaggtct
     7501 ttatcaagct gcgcgttctg ttgcatgacg actccatcgt gtcagaatgt gtttttcgta
     7561 ttcactcatc gtgcggtcct tacggcgtga gatgcgcttt gctatccggg caatgatcgc
     7621 cctgatggct ttcctcagga catgaagttc gcgttccatc cggcgaatat accgttcttc
     7681 ctcgcgccgc ttctcgatga gcgttgccgt ttcttttacc ttcttctcgt cgtcggtccc
     7741 tacatactgc gagagtatcc gggaaccttc ccgatattga aggtaccagt agccccggcc
     7801 gttcttcagt ttccgggaca gatacccctt ggaagcgccc gcgacgaatc tccgggccgc
     7861 ttcaagagag tcggtccact gatcgtactg gatcatcatc caagtgagtg ctgcgtatct
     7921 catgattgac cgcacctctc atatacccgg atcgcgatgg agagaatggc gacatcctct
     7981 ttcaattcgc gcaggcgttg tttgagcgca cgaagggacg cgaacccgct atcggcgata
     8041 tcgccgttgg ccagcctgcg actcaagagt tcgatctgct cctgttcccg tgcgtgctcg
     8101 tttttcaact gttcgaacat ggtgttcctc actatgtacc ttttttataa tacctgtagg
     8161 gtaacgaatg tcaagaagtc tttttcattt tgcacctgta tttcgtattt tgtagggtaa
     8221 caagggaagg gccgcccgag acttgaccgg acggcccatc tctctagaac ggtacggtct
     8281 gatcgaccag ttcgaggccg cgatcgcgcg cgatgtcggc ggcgaccgtg gccgccttct
     8341 cgaacgagtg gcagatgtag gtcttgaggg cgttcagccc ttcggtgaga atggtgacct
     8401 tgaaggtctc gatgtcggtc cccggtttct tgaccgacgg gactttttcg ggaccggtca
     8461 ggatgacttt ggtggctttg gggttctgtt ccatggcttt tctccttaga aaagtggttg
     8521 ttgttggttg gtcggggaga aaaccttctc tccgacggaa atgttgttgt ttcagggatg
     8581 ccgtgacgcg acggtcaggg cgagcggccg ggtagggacc gctatggcgg tttgtgcggt
     8641 acgcatggta agtccggtcg cgatcacatt gtccacgatg aaggcgttcg ccggtacggg
     8701 atgaacctgc ttcatcttca cggtcacttt cttgcgctgt tttttcgcga ggtaaagact
     8761 catacgacgg ttcccgcgaa gagcgtctat gaccgtgccg ccggaaagtc gcgctaaata
     8821 ccagcagaga tcccgcatcc aagaggaggg cttgccggta tggctcggca tgggaataaa
     8881 tacgggggag gattcctccc gtatgcggtc ccatatcagg aaggcggctg aggggatgcc
     8941 gttcccttcc ttgatcgagt gggcgagggt tcggtagcgg taactcatgg gttcctcctt
     9001 gggatgcgcg cctcgtaatc gggcgcaata ctgtgttgct gcctccctcg ctctcctcgg
     9061 agtagcgctg ctacacctgc gtggctcggt cgtcgcgccg tgtcttgcgc tccgacccga
     9121 ggcgcttaaa agcggggagg ggacggttac tcttcggtct ccttgcgata ccgaagcggg
     9181 ttgttgttga cttgtgttgt tccgacccga ggcgcttaaa agcggggagg gggttggtgg
     9241 gtgttgttgg ctgtcggcct gcctgcaacc gaggccgata tggccgccgt atcgcgtccg
     9301 tcaggtgatg ccgttatatg aggccgctct ggcggaacga atagtgagaa tcgaccgtga
     9361 tgatcacatg gtcgagaact tcgatgccga gtatcttgcc cgactcgatg agacgcttgg
     9421 tgaccgcaag gtcttcggcg gacgggtcgg gagtgcccgc agggtggttg tgggcaagga
     9481 tgaccgacgc ggcgcgtaac tctatcgcgg gcgcgaaggt ttcgcgtgga tgaacgagcg
     9541 actggttgag cgtaccgatg aagatgacgc gcgtgtcgat cgggtgtccg gcggcatcga
     9601 ggtagatgac gacgaagttc tcctgttgcc agttggtgat caccgtggcg gccgtcttga
     9661 cggcgtcctc ggagtttttc acattgggcc gcttttcgcc gatgaggttg gaagaggcga
     9721 ggagcgagag gagttgtttc ttggtcatgc ggaagtattc cttcttcata tcgggtctcc
     9781 ctcgctcgaa agaagccagc aggcgaagag aagaacggag atgatcaggt tttccatgat
     9841 tctcctccct agtcttcgat gtcttcatct gacaggtagc cgttatcggc gagccagtca
     9901 gcgtgattga ttatgtcgcc atcttgattc atgtgatact gttccatgat tgcctccaaa
     9961 agccccaacg ggggcgttgt gttgttgtgt taaagtgtta aagtgatact gtgatactgt
    10021 ctttgcccga ctctcctgtg ccgtcctct
//
LOCUS       DAOQUE010000037         9970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_7585_length_9970_cov_4.583459, whole genome shotgun sequence.
ACCESSION   DAOQUE010000037 DAOQUE010000000
VERSION     DAOQUE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7585_length_9970_cov_4.583459"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="PKH10_02080"
     CDS             complement(<1..1179)
                     /locus_tag="PKH10_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26944.1"
                     /translation="MYRSFLALSLLFATALPAQEIWTVQQPGEMTTADFYLLENGSRT
                     TGLLIGKKNSGSNDELLCFVMVTGYQWNPCPPGFTSFAGMSGAFITGAKYGSPSTLYA
                     IRTEIQGMSSVTALVRTDAFLNNMIVLKVFNNDTEEMNGSLAILGDTIWAGTNDGKVL
                     RSTDGGTTFETFTVTTDTEVGITYLHFTDAQNGYAAGGATKEEDTGEGTITTVLSKGG
                     IWTTTDGGASWTAVKEGLPYSFLKVAPVTTGGGGDLTNVTWYAFYTDDESYSGGDVSK
                     HVGVTSDNFATLTIAEPQANTGKTFGGFQAAGVDIIDGTDIWLGGVCNDFKACSIVSF
                     DGGTTWLEMFMPADVGVSFMWSLKAQNFLDSKHGWAAGSVNTILRWGDPNEDLTEQPD
                     T"
     gene            1315..3546
                     /locus_tag="PKH10_02085"
     CDS             1315..3546
                     /locus_tag="PKH10_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26945.1"
                     /translation="MMRVFGIFAVSTVLFFFAACDKKETAATDDLTTDETVDDDDGTD
                     QTLLEGDEGESDQSDTSDISDNMVDEDSALSDDLLADDQPGPTDETDGSDQSDDISPD
                     EDLAGLVLPAETTCQPSAAPCDAAPVETNIYASYRKDFYYPNYQEGDVINPIPAPTSG
                     GRFQITGIAGKGGTVTAVKLNGVNVADLLTQTKLDWYHVYPLTLTEGEAFWVNFHSRE
                     ASWDSASNGQLTVETGEGTAFSGPFNYAVNQVPLKYVAFADDYQTLIIHIENRTQNPV
                     TLTKLLYNGRDVTAAACMAGDILQPNVNMLITVPLCEAVSPGDPWTVTALVNGIGPST
                     AGGRVIRDFYPIETWQSDDECPYYPGYNEAWYTDHRAHGFDTFFMRPTSAGECSSNFP
                     GGTGDIYELLGPIFEQYGLQAVLTFEANNQFLDYTDVSNIAVAFHADECDDNLLDNGY
                     PKPETRAQYARRTWETWPDLPTYIGGSRGRYNGAFAGATDIQGFDYYVAACAPHITVA
                     GTHPPLRGAFDQLLLVHENHMPHTTWAYTQGLHNGSWNATIPGIGTKVYRQPTPAEFR
                     IQALSVLMVGSKGIMYFQTDKEMVDMFPATWEEIGKTNREIHGLKPYLREGDIHAIIQ
                     QEGQFIASVIRSREMMALVLFSTHVTNTPTEQQCLTAQNVHWAWGDTTLVTVPVAVPP
                     EFPVAEVWELRDGQFLPANYSVNGRTVTFPDIALNEQNVGRIFVLISDPAIKDDMLGR
                     MEY"
     gene            3661..4863
                     /locus_tag="PKH10_02090"
     CDS             3661..4863
                     /locus_tag="PKH10_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT26946.1"
                     /translation="MGRRNFLKRVSLRNYKSIAECDVDLGALTFLVGRNGAGKSNFLD
                     ALHLVTESLTSSLDHALRNRGGINEVRRRSSGHPTHFQIRLDIQTDLFDGYYSFRIGA
                     KSKGGFEVQKEECLINKLLRKDSYRVEEGTVIESSAEVMPPASPDRLYLVSAAGLPEF
                     RPVFDALSHMGFYNLNPDAIRELQTPDTGEVLRRDGSNLASVLSVMEKESPESRKYVV
                     DFLSKVVPGISDVSSKRLRNKETLDFRQQVGSNKASWPFSAQNMSDGTLRALGVLTAI
                     FQSNTENGSWIPLVGIEEPETAVHPGAAAVLRDALRVASASKQVLVTSHSPDLLDDKD
                     IEADEIVAVENDNGKTLIGPLTETDRGLLKDKLFTAGELLRQGGLLPDREKASRVIQE
                     NADGHTNS"
     gene            4860..5468
                     /locus_tag="PKH10_02095"
     CDS             4860..5468
                     /locus_tag="PKH10_02095"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HNT26947.1"
                     /translation="MNPIHIVAIVEGHGEETALPVLIRKIARENNIYNQLVIKPVFRI
                     PATRLKKEGELERQVELAARSLPSENGAILILVDCDGENDCPAQEGPALLARAQAARK
                     DKLISVVLAKKEFESWFIAAASSLRGVNRLSQTMPEPDDPESIRGAKEWLSGFMPQAF
                     PYAATIDQERLTIHFDMTLARRADSFDKCYREIVKMLRYLAG"
     gene            5478..5630
                     /locus_tag="PKH10_02100"
     CDS             5478..5630
                     /locus_tag="PKH10_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26948.1"
                     /translation="MAIDYYLEEVAFWAEAKQRLNSNEETVTHAEVLRKLGLSQSACG
                     RSSALS"
     gene            complement(5640..5978)
                     /locus_tag="PKH10_02105"
     CDS             complement(5640..5978)
                     /locus_tag="PKH10_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26949.1"
                     /translation="MIICNLVHYLFPEWYANGLFFLFSFLLGIPLIIIWFAMARKRIL
                     KDWRFRLYSGLFSVLYIISLIFIPEGSGNSTAGIGVAASIIDMPIIDVAIYLVIRFFA
                     EPEEPKSGQP"
     gene            complement(5992..>9970)
                     /locus_tag="PKH10_02110"
     CDS             complement(5992..>9970)
                     /locus_tag="PKH10_02110"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNT26950.1"
                     /translation="TVEGKAISYAGAPLSDAKVQWYVEREVQFPYFFGLWGRSWFYDL
                     WNGKKAIIGKGETTTGPDGRYRISFTARPDTQVPKEEQPLFSYRVHAEVTDLNGETHP
                     AERFVQAAYSALRITVGGAAQGFVNAPFQLELATTNLSGEPEPSKGEVTVSRLADPER
                     LLRPRLFYERPDRSLLDKTAFVKQFPHDPYGDEERAPRPKEKEYLRRPFDTGAEKKMT
                     IDNKDWPAGDYLVEVSAQDKFGQPVTTRRIVPVRAVGDGAYARNRYDDIAVVKESYEP
                     GETAEIIFASAADAVTVVLDILRGEKEERTILALDRSQKVVRIPVTESDRGNIAVRYL
                     FLKHGRSFMGMRTIAVPWTNKQLSYEFLSFRSTLKPGEKEEWRIKLKGAKGEAVAAEL
                     AATLYDGSLDAFLPHQWAFSLFPTHYYRYQWLTNESQQIRYAQPLERDWNTYVAWPVL
                     TSDAIHYFGMYLGGYGYRGRGGGGYGGVVAGAMRMAMTETAADEESKPTDSAGAAAPM
                     VQGRVAKPEINTFSRNAANGASRYEFDDMDLSGELLKPAPPPVQIRKNLQETAFFYPQ
                     LMTDEKGETIVSFTVPEALTTWKMIGLAHTKELESVILKNELKTQKELMVAPNMPRFL
                     REGDRLILSAKISNMTDAVQTGSAELKLLDARTMNDVGATFALAQKDADFTVPAKRSG
                     AVKWEVTVPRRDDPVVWRIVAKAGDTGDGEENILPILSDRMLVTETMPLPVNGREKKA
                     FTLEKLKSNASPTLVNHRLTLEFTSNPAWYAVQALPYLIEYPYECAEQVFSRFYANSL
                     ATHIVKKNPKIEKVFAQWKGTPALDSNLMKNEELKSALLQETPWVLDAQDEAASKRRI
                     ALLFDLNRMAGELKSAFDKLRRLQVSSGGWPWFPGLPEDRYITQHITAGLGKLKKLGV
                     AAGPDIDRMTRAAVGYLDRQMERDYQELLRAKVDMTKEHISYIERHYLYTRSFFTDIP
                     FAEQHKTAFNYWKGQATTYWLAETPYMTALTATALFRFGDAAAAAKMIVSLKERAIYS
                     DELGMYWKENMGGYYWYQLPIETQAALIEAFEEVAKDGRSADKMKTWLIKMKQVQNWR
                     TTKATADAVYALLHSGTDWLSASKLATVTVGGKLARPAEGERTPEAGTGYFKTAWPGK
                     EVTPAMAQVTVENPNPGPAWGALYWQYFENLDKITADKQGPLALERQILREKRTDSGA
                     VLEPITEQTPLTVGDKIAVRIVLKVDRDMEYLHLKDARPAGTEPVEVLSGSDYRDGLW
                     YYRVTKDVSTNFFIHRAVKGTYVLEYRLTVTIAGNYSAGLATAQCMYAPEFAAHSAGL
                     RLTAQ"
BASE COUNT         2006 a         3015 c         2889 g         2060 t
ORIGIN      
        1 ggtatcgggc tgttcggtga gatcctcgtt cgggtcgccc caacggagga tggtattgac
       61 gctccccgcc gcccagccgt gcttcgaatc gaggaagttc tgcgccttga ggctccacat
      121 gaaagagacg ccgacatcgg ccggcatgaa catctccagc caggtggtgc cgccgtcgaa
      181 actgacgatc gaacaggcct tgaaatcgtt gcagacgccg ccgagccaga tgtcggtccc
      241 gtcgatgata tccacgccgg ccgcctgaaa gccgccgaag gtcttgccgg tgttcgcctg
      301 cggttcggcg atggtgagcg tggcgaagtt atcgctggtg accccgacat gtttcgaaac
      361 atcccctccg ctgtaggatt cgtcgtcggt gtagaaggcg taccaggtga cattcgtcag
      421 atcaccgccg ccgccggtgg tgacgggtgc tactttcagg aaggagtagg ggagtccttc
      481 cttcacggcc gtccatgagg cgccgccgtc ggtcgtggtc cagatacctc ccttgctgag
      541 gaccgtcgtg atggtccctt cgccggtgtc ctcctctttc gtcgctccgc ccgcggcata
      601 gccgttctgc gcatcggtga aatggaggta ggtgatgccg acctcggtat cggtcgtgac
      661 ggtgaaggtc tcgaaggtcg tgccgccgtc ggtggaacgg agcaccttgc cgtcattggt
      721 gccggcccag atcgtatcgc cgaggatcgc cagcgagccg ttcatctctt cggtgtcgtt
      781 gttgaacacc ttgaggacga tcatgttgtt gagaaaggcg tcggtccgca cgagtgccgt
      841 gacgctcgac attccctgta tctcggtccg tatcgcgtac agcgtcgagg gcgagccgta
      901 tttcgcgccg gtgatgaagg cgccgctcat accggcgaac gaggtgaagc cgggcgggca
      961 ggggttccac tggtagccgg tcaccatgac gaaacaaagc agttcgtcgt tcgatccgct
     1021 gttcttcttg ccgataagga gcccggtggt gcgcgagccg ttctcgagca gatagaagtc
     1081 ggcggtggtc atctcgcccg gctgctggac cgtccagatc tcctgcgccg gaagcgccgt
     1141 cgcaaaaaga agagaaagcg ccaagaatga acggtacata gggttcctcc acggtggtgt
     1201 caacagaaaa aacataggag cgcaccgtgt tttgtcaagt tttgacagct cgctctcttt
     1261 ttggtatggt cgccgcgaca ggaacaaagg aacaactttt caagggagat gacgatgatg
     1321 cgggtgtttg gtatctttgc cgtatccacg gtgctgttct tttttgcggc ctgcgataaa
     1381 aaagagaccg ccgcgaccga cgacctgacg accgacgaaa cggtggatga cgacgacggc
     1441 accgaccaaa ccctgctgga aggcgatgag ggagaaagcg accaaagcga caccagcgac
     1501 ataagcgaca acatggtcga tgaggacagc gcgctgtccg acgacctgct tgccgatgat
     1561 cagcccggcc cgaccgatga aaccgatgga tccgatcagt ccgacgacat atcccccgac
     1621 gaggacctcg ccggactcgt tctccccgcc gaaaccacct gccagccctc ggccgccccc
     1681 tgcgacgccg cgccggtaga aactaacatc tacgcctcct accgcaaaga tttctactat
     1741 ccgaactatc aggaaggcga cgtcatcaac ccgataccgg cgccgaccag cggcggacgc
     1801 tttcagataa cgggcatcgc cggaaagggc ggcacggtga cggcggtgaa actgaacggc
     1861 gtgaatgtcg ccgatcttct cacccagacg aaactcgact ggtaccatgt ctacccgctg
     1921 acgctcaccg agggggaggc cttctgggtc aatttccaca gccgtgaggc gtcgtgggac
     1981 agcgcatcga acggtcaact gaccgtcgag accggggagg ggaccgcctt cagcggccca
     2041 ttcaattacg ctgtcaatca ggtgccgctc aagtatgtcg ccttcgccga cgattatcag
     2101 acgctcatca tccatatcga gaaccggacc cagaacccgg tgacgctcac caaactcctt
     2161 tacaacggcc gcgatgtgac cgccgccgcc tgcatggcgg gcgatatcct gcagccgaac
     2221 gtcaacatgc tcatcaccgt gccgctctgt gaagcggtct cgccgggcga cccgtggacc
     2281 gttacggcgc tcgtgaacgg catcggcccc tcgaccgccg gcggccgggt catccgtgac
     2341 ttctatccca tcgagacctg gcagagcgac gacgaatgtc cctactaccc cggttacaac
     2401 gaggcgtggt acaccgacca ccgcgcccac ggcttcgaca ccttcttcat gcgtcccacg
     2461 agtgcgggcg aatgttcgtc gaactttccc ggcggcaccg gcgacatcta cgaacttctc
     2521 gggccgatat tcgaacaata cggtctgcag gcggtgctca ccttcgaggc caataaccag
     2581 ttcctggact ataccgacgt ctccaacatc gcggtcgcct tccacgccga cgaatgcgac
     2641 gacaatctgc tcgacaacgg ctatccgaaa cccgagacgc gcgcccagta cgcccgtcgg
     2701 acctgggaga cctggcccga cctgccgacc tacatcggcg gttcgcgcgg ccgctacaac
     2761 ggcgccttcg ccggggcgac cgacatacag ggcttcgact actatgtcgc cgcctgcgcg
     2821 cctcacatca ccgtcgccgg gacccatccg ccgctccggg gcgccttcga ccaactgctc
     2881 ctcgtccacg agaaccacat gccgcacacc acgtgggcct acacgcaggg tctccacaat
     2941 ggcagttgga acgccaccat cccgggcatc ggcaccaagg tctaccgcca gccgaccccc
     3001 gccgagttcc gcatacaggc gctctcggtc ctcatggtcg gctcgaaggg gatcatgtac
     3061 ttccagaccg acaaggagat ggtcgacatg ttccctgcga cctgggaaga gatcgggaag
     3121 accaatcgcg agatacacgg cctcaaaccc tacctgcgcg agggcgacat ccacgccatc
     3181 attcaacagg agggtcagtt catcgcgtcg gtcatccgat cacgcgagat gatggcgctc
     3241 gtcctcttca gcacccatgt caccaatacg ccgaccgagc agcagtgcct cacggcgcag
     3301 aatgtccact gggcgtgggg cgacacgacg ctcgtcacgg tgccggtcgc ggtgccgcca
     3361 gaattcccgg tcgccgaggt atgggaactc cgcgacggcc agttcctgcc ggcgaactac
     3421 agcgtgaacg gccgcaccgt caccttcccc gacatcgccc tcaacgagca gaatgtcggc
     3481 cggatattcg tgcttatttc cgatccggcg atcaaagacg acatgctggg aaggatggag
     3541 tactgaaaag ggtcactgcg ccgtgaggcg cgatccggtt ttatattgac aaaacaaccc
     3601 gcttttcata ccctcaacgg acgatagatg ttgtttgcga caagagatgg gaggaaatca
     3661 atggggcgca ggaatttcct gaaaagagtt tctctcagaa attataaaag cattgcggag
     3721 tgcgatgttg atttgggggc cttgactttt cttgtcggcc gaaatggtgc cggcaaaagc
     3781 aattttctcg acgccctaca tcttgtaacg gaatctctca cctcttcgct tgaccatgcc
     3841 ttaagaaacc gtggcggtat taacgaagtg agaaggcggt cttccggcca cccaacccac
     3901 ttccaaatac gccttgatat tcagacagac ctttttgacg ggtactactc ttttcgcata
     3961 ggagcaaagt cgaaaggagg tttcgaggtt caaaaagaag aatgccttat caacaagctt
     4021 ttaagaaagg actcttatcg cgttgaagag ggcacagtca tcgaatccag cgctgaggta
     4081 atgccaccgg caagccccga tagactttat ctcgtcagcg cagccggact acctgaattt
     4141 cgtcccgttt tcgatgcgct ctcgcacatg ggtttctata atctgaatcc cgacgcaata
     4201 cgagaactac agacacccga taccggcgag gtcctacgca gggatggcag taacttggca
     4261 agcgtgctgt ccgtaatgga aaaagaatct cccgaaagtc gaaaatatgt cgtggatttc
     4321 ttgtccaagg tcgttccggg tatctcagat gtttcttcca aacgattaag aaacaaggaa
     4381 actctggatt tccggcaaca agtaggatcc aacaaggcat cttggccgtt ttcggcacaa
     4441 aacatgtccg acggcacatt gcgtgcgctt ggcgttctca ccgctatttt ccaaagcaat
     4501 accgaaaacg gttcttggat cccattggta ggcatagaag aaccggaaac cgctgtgcat
     4561 ccgggagcgg ccgccgtttt acgggatgcg cttcgagttg cgtcggcatc taaacaggtc
     4621 ttagtaacga gccatagccc cgacctgttg gatgataaag atatcgaggc agacgaaatc
     4681 gtggctgtgg aaaacgataa tggaaaaact ctgataggcc cccttaccga gacggatcgt
     4741 ggtcttttaa aagacaagtt gttcactgct ggcgaacttc ttcgccaagg aggattgctc
     4801 cctgaccgag aaaaagcttc tcgtgtgatt caagaaaacg ccgacggaca caccaattca
     4861 tgaaccctat tcatattgtc gccatcgttg aagggcacgg tgaagaaact gcgcttccgg
     4921 tccttattcg aaaaattgcc cgggaaaaca acatttataa tcagcttgtc atcaagccgg
     4981 tattccgcat cccggcaact cgtctaaaaa aagagggcga acttgaacga caagttgaac
     5041 tggcggcgcg atctctgcca tcagagaacg gtgccatctt gatacttgtt gattgcgacg
     5101 gagagaatga ttgtccggca caggaaggcc cagcattgct tgcgcgcgca caagccgcaa
     5161 gaaaagacaa acttatttcg gtcgttctgg caaaaaagga gtttgaatcg tggtttatcg
     5221 ccgccgcttc ttcattacgc ggagtgaatc gattatctca aaccatgccg gaaccggatg
     5281 atcctgaatc tattcgcggc gccaaggaat ggctcagtgg atttatgccg caggcatttc
     5341 cctatgcagc aactattgat caagaaaggt tgacgattca ttttgatatg actcttgcgc
     5401 gtagagccga ctcattcgac aagtgttata gggaaattgt gaagatgctt cgatacctag
     5461 cgggataact tgcgatcatg gctatcgact actatctgga agaggtcgcg ttctgggccg
     5521 aggcaaaaca acgcctcaac tcgaacgaag aaacggtcac gcacgccgag gtcttgagaa
     5581 aactcggcct ttcgcaatct gcctgcggcc ggtcgtcggc actttcatga ccatcgtctc
     5641 tatggctggc cggatttagg ttcctcgggt tcggcaaaga atcgaatgac gagataaatt
     5701 gcgacatcta ttataggcat atcgatgatt gaggcggcca cccctattcc ggctgtagaa
     5761 ttgccactac cttcaggtat aaatataagg gaaatgatat ataaaacgga aaacaggcct
     5821 gaatataatc taaaccgcca gtccttcaaa atcctttttc ttgccatcgc aaaccaaatt
     5881 atgattaaag gaatacccaa gaggaaagaa aacaggaaga acaaaccatt cgcataccat
     5941 tcaggaaaaa ggtaatgcac taaattgcag atgatcattc tcttctttcc tttattgtgc
     6001 ggtaagccgc agtccggcgc tgtgggcggc gaactcgggg gcgtacatgc actgcgccgt
     6061 ggcgagcccc gccgaatagt tcccggcgat ggtcaccgtg agccggtatt cgagcacata
     6121 ggtccccttg acggcgcggt ggatgaagaa attggtcgat acatccttgg tcacgcggta
     6181 gtaccagaga ccgtcgcggt agtcggaacc cgacagcacc tcgaccggtt cggtccccgc
     6241 cggacgggcg tccttgaggt gaaggtattc catgtcgcgg tcaaccttga ggacgatccg
     6301 caccgcgatc ttgtcgccca ccgtgagcgg tgtctgctcg gtgatcggct ccaacaccgc
     6361 gccactgtcg gtccgcttct cgcgcagtat ctgccgttcc agcgcgagcg gcccctgttt
     6421 gtcggccgtt atcttatcga ggttctcgaa gtactgccag tagagggcgc cccaggccgg
     6481 gccggggttc gggttctcca ccgtcacctg cgccatcgcc ggggtcacct cctttccggg
     6541 ccacgccgtc ttgaagtagc cggtgccggc ctcgggggtc cgctccccct cggcgggacg
     6601 ggcgagcttg cctcccaccg tcaccgtcgc gagtttcgag gccgacagcc agtcggtccc
     6661 gctgtggaga agcgcgtaga ccgcgtcggc ggtcgccttg gtggtgcgcc agttctgcac
     6721 ctgtttcatc ttgatgagcc aggtcttcat cttatcggcg gagcggccgt ccttcgccac
     6781 ctcctcgaac gcctcgatga gcgccgcctg cgtctctatc gggagctggt accagtagta
     6841 gccgcccata ttctctttcc agtacatccc gagctcatct gagtagatcg cccgctcctt
     6901 gagcgagacg atcatcttcg cggccgccgc cgcatcgccg aaccggaaca gggccgtcgc
     6961 ggtgagcgcc gtcatgtagg gcgtctctgc gagccagtag gtcgtcgcct gccccttcca
     7021 gtagttgaag gccgtcttat gctgttcggc gaacgggatg tcggtgaaga aactgcgggt
     7081 gtagagataa tgccgctcga tgtaggagat gtgctccttg gtcatatcga ccttggcgcg
     7141 caggagttcc tgatagtcgc gttccatctg ccggtcgagg tagccgaccg ccgcgcgggt
     7201 catccggtcg atgtcgggac cggccgcgac gccgagtttc ttgagcttgc caagccccgc
     7261 ggtgatgtgc tgggtgatgt agcggtcctc gggcagcccc gggaaccacg gccagccgcc
     7321 gctcgatacc tgtagccgcc gcagtttgtc gaaggcgctt ttcagttcgc ccgccatccg
     7381 gttaagatcg aacaggagcg cgatgcgccg cttggaggcc gcctcgtcct gcgcgtcgag
     7441 cacccatggc gtctcctgca ggagcgccga cttcaactct tcgttcttca tcaggttcga
     7501 gtcgagcgcg ggcgttccct tccactgcgc gaagaccttc tctatcttcg ggttcttctt
     7561 cacgatgtgg gtcgcgaggc tgttggcata gaagcgcgag aagacctgct cggcgcattc
     7621 gtaggggtat tctatgaggt agggcagcgc ctgcaccgcg taccacgccg ggttcgaggt
     7681 gaattcgagc gtgagccggt ggttgaccag cgtcggcgag gcgttgctct tcagtttctc
     7741 gagcgtgaac gccttcttct ccctgccgtt caccgggagc ggcatcgtct cggtgacgag
     7801 catccggtcg gaaaggatcg gcaggatatt ctcctcgccg tcaccggtat cgcccgcctt
     7861 cgcgacgatg cgccacacga ccggatcgtc gcgccgcggc accgtcacct cccacttcac
     7921 cgcgccgctc ctctttgccg ggacagtgaa gtccgcatcc ttctgcgcaa gggcgaaggt
     7981 cgcgccgaca tcattcatcg tccgggcgtc gagcagtttc agttcggcgc tccccgtctg
     8041 caccgcgtcg gtcatgttgc tgatcttcgc cgaaaggatg agtctgtccc cttcgcgcag
     8101 gaagcgcggc atattggggg cgaccatcag ttccttctgc gtcttcagtt cgttcttcag
     8161 gatgaccgac tccagttctt tggtgtgggc gagaccgatc atcttccagg tcgtgagcgc
     8221 ctcggggacg gtgaacgaca cgatcgtctc ccccttctcg tcggtcatga gttgcggata
     8281 gaagaaggcc gtttcctgca ggttcttgcg tatctggacc ggtggtggcg cgggcttgag
     8341 gagttccccg gagaggtcca tatcgtcgaa ttcatagcgg ctcgcgccgt tggccgcgtt
     8401 cctgctgaac gtatttatct cgggtttcgc gacacgaccc tgcaccattg gcgctgcggc
     8461 gccggcgctg tcggtcggct tactctcctc gtcggccgcc gtttcggtca tcgccatccg
     8521 catcgcgccg gcgaccacgc cgccgtaacc gccgccgccg cggccgcgat agccgtagcc
     8581 gccgaggtac atgccgaagt aatgtatcgc gtcgctggta agcaccggcc acgcgacata
     8641 ggtgttccaa tcgcgttcga gcggttgggc ataacggatc tgctgacttt cgttggtcag
     8701 ccactggtac cggtagtagt gcgtcgggaa aagagagaag gcccactggt ggggaaggaa
     8761 cgcatcgagc gagccgtcgt agagcgtcgc cgcgagttcg gccgcgaccg cctcgccttt
     8821 cgcccctttg agtttgatgc gccactcctc tttctcgccc ggcttgaggg tgctgcggaa
     8881 cgaaaggaac tcgtacgaga gttgtttgtt cgtccagggc actgcgatgg tgcgcatccc
     8941 catgaaacta cggccatgct tgaggaagag gtaccggacc gcgatgttgc cgcggtccga
     9001 ctcggtcacc gggatgcgga cgaccttctg cgaccggtcg agcgcgagga tcgtgcgctc
     9061 ctccttctcg ccccgcagga tgtcgagcac caccgtcacc gcatcggcgg cgctcgcgaa
     9121 gatgatctcg gccgtctcgc ccggttcgta gctctccttc accaccgcga tatcgtcgta
     9181 ccggttccgg gcgtaagcgc cgtcgccgac cgcgcggacc gggacgatgc gccgcgtcgt
     9241 caccggctgg ccgaacttgt cctgcgcgct cacctcgacc agatagtcgc ccgcgggcca
     9301 atctttgttg tctatcgtca tcttcttctc ggcgccggtg tcgaagggac gacgcagata
     9361 ttccttttcc ttcgggcgcg gagccctctc ctcatcgccg taagggtcgt gcgggaactg
     9421 cttcacgaaa gccgtcttgt ccaagaggga acggtcgggc cgttcgtaga aaaggcgcgg
     9481 ccgcaacagc cgctcgggat cggcgagccg cgaaacggtc acctctccct tcgacggttc
     9541 gggctcgccg ctcagattgg tcgtcgcgag ttccagttgg aacggcgcgt tcacgaaacc
     9601 ctgtgccgcg ccgcccaccg ttatccggag cgcgctgtag gccgcctgca cgaaacgctc
     9661 ggcggggtgc gtctcgccgt tgagatcggt cacctcggcg tgaacgcggt aggagaacag
     9721 cggctgctcc tccttgggca cctgtgtgtc gggccgcgcg gtgaaggata tccggtagcg
     9781 accgtcgggg ccggtcgtcg tctccccctt gccgatgatc gccttcttgc cgttccagag
     9841 gtcgtagaac caactccggc cccagagacc gaagaaatag gggaactgca cctcgcgttc
     9901 gacataccac tgcaccttcg cgtcgctgag tggcgccccc gcgtaggaga tcgccttgcc
     9961 ttcgaccgtc
//
LOCUS       DAOQUE010000038         9937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_7621_length_9937_cov_4.280712, whole genome shotgun sequence.
ACCESSION   DAOQUE010000038 DAOQUE010000000
VERSION     DAOQUE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7621_length_9937_cov_4.280712"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKH10_02115"
     CDS             <1..362
                     /locus_tag="PKH10_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26951.1"
                     /translation="STEVFYEIQKYMFNSPDIAKKILSHRDTELNWLNGVMAEYLEES
                     PDDDRTRKITLMYAAFLHSFVTMSAGIAKQAKALESYSIISQFPWRLDNIDHSDIFEF
                     LIGGVDDLKKKLFGTQG"
     gene            373..1539
                     /locus_tag="PKH10_02120"
     CDS             373..1539
                     /locus_tag="PKH10_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT26952.1"
                     /translation="MNTRSVSVMLTLMTACGFFLFLSCSGKPEGNGAEAVQAVTITKP
                     EKKKFSRSFDTAAVAMAERSAYINPKVAAIIRSFRVKEGDFVKEGALLAQLDGSDYEI
                     AVNASRAQLAAAEAGVMQAEAAFAKISADFERFKALKQNGSISASDFEQMEAGYKQTE
                     AGLAAAKAQRGMAQSALDGNLRQLGYTALIAPFTGYIATRNGEIGEMAAPASPRPMFE
                     IVQSDTLKIQVFISELEMRGVTKDTVAKVFFDAYPDKEASAKVNLINSKVDPMTKSVK
                     VELVMENRDHFFKPGMTVRVRFTLPEKEYLVVPRNAVFTRDNEAGIVYVKDADDRVFT
                     KEIFMGGAVEGYTIVEKGLDGAEDIVVGGGRRLEEGQKVRVVTPGTAVAGEVRK"
     gene            1536..4646
                     /locus_tag="PKH10_02125"
     CDS             1536..4646
                     /locus_tag="PKH10_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT26953.1"
                     /translation="MNLPKFAVKRPVLVSVIFVMMGLFGVYSLMDLPIDLMPDIEAPA
                     ISVITIYRGAGSEEVEEKVSRVIESALASTAGMKNIYSRSMENVSSVTCEFEFGVDLA
                     EATNKIRDLLNMSRSFLPDDIDEPLVFKFDFSMMPIMFLAITSSKEDIRYKGEEIDNY
                     ITNYFERIPGVGSVITFNEMKKKIIISAEKQRLSALNLSVDDLVNTIRAENLSLPAGN
                     IEIGSYDYTIRVPGEYKSIEEINETIIASTPAGLIRIKDVARVFWGSEDSRQYGIKDG
                     EYMVFMMVQKQSGANTVSIADAVKKEIEKIRPRLPDGYRIGILLDSSDFIVKMIRNLS
                     TAVLTACLFVLLVVVFFLRRFRSSFIVLLSIPASMIIAFAFLYGFGFTLNLVSLMSLS
                     LAIGMVVDNSIVVLDNITRYLESGKSRADAAIEGTSEVGGAIVASTLTTIVIFAPLFF
                     VGGLIGILFGQLAGVIVLTLSASLLAAMLLTPMISSRILTTQGNGGSYDNWFFRIGER
                     FLTFVEDRYTRVIRLTLQHKKKTILVAGLTFLGSLMIVPLIGVDFMPEGDEGLIQIEY
                     ELPLGSRIEETLKVGQHIERIIREEVPERYLERVFLRGGPDDSGFAQKVQDTNTGLTG
                     AKLVSQNFRKEGIKFFANKIRARIIAEVPGIEKMDVKTSSGFGDLISGGEKPVTVKLA
                     HKDFKKLNEAAARLEKEFQKIAGLKDIGNDADSLKPQIEVKIDRIRASQVGLKTAMIA
                     AAVRNAFYGSTASVYRKDGDEFEIMVKYKKADRENIEQLKELEIKTMYGTVVKLKDVA
                     EVAEGLTSLSVNRQNRERIVSVGARLEEDVAIGKIGAEVEKAIERANIDPEIGIIYGG
                     TLKSQKENTGDLVRMLLLGILLVYLVMAAQFESFVDPFVIIFSIPFAISGVLIGLLLT
                     GHTLSVPAFLGMIILVGIVVNNAIVLIDYTKIQQEKHRFGTDESLIYSGARRLRPILM
                     TATTTIFGMLPLALVSGEGHETYNPMGVAVVFGLAFSTLVTLVLIPCMYSTIDGFLRK
                     RGWRTD"
     gene            complement(4665..5546)
                     /locus_tag="PKH10_02130"
     CDS             complement(4665..5546)
                     /locus_tag="PKH10_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HNT26954.1"
                     /translation="MSRPKKDRTVKRAATDIFQHTRYRTYFRSVYEELKREEKGFSIR
                     SFQRSAGLSDRSNHFWQVINGTVRLSLTSAERYARALGLTKEEKGHLQAMIALEKAKT
                     EEERERIIRAMIASSRFLSGDRYLTAALVLFSDWKLPILWEVVALDTFREDPSWIAKN
                     FFTGIAPAAVREGLDKLLAHGFISRDRQGKLRPERINLSDYVAAAREKNDELVTIRKA
                     QTRNGMTKALEALDNQPFEKRLFYGMSFAVSKELYEEMKQRVIALQEELRLIAAKRPV
                     RDAVYQLNVQFFAPFSS"
     gene            complement(5543..7312)
                     /locus_tag="PKH10_02135"
     CDS             complement(5543..7312)
                     /locus_tag="PKH10_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26955.1"
                     /translation="MKKIFALLFASVLGVLVCSACTPQEEDTDIDNDGLASTDNEQGD
                     GGILPDDPSSDDPQSDGLIPDTDTVNELCTDPNGDEDGDGIPNGVEVNVDTDLDGAPD
                     CLDADADGDGILDSAECPSQPCRDTDGDEKPDYLDKDSDGDGLSDKDEVAAGTDPTKK
                     DSDDDGSDDLAEIVYGSDPTDDTDTIPDGLFFVVLPYNGHEANRTLEFSTVIEAIDVL
                     FVIDASGSMYGEIDRVQEEVKTNIIDKINAQYADPDFAAFGLSRITFDGTANFLRQKM
                     TLDADEIKASLDNILPPADQKFSIGISEMASEPLYLSFSDEQFHGHASLCPPGMGCIA
                     EAEIYVPPADCTGALGSIGHACLRKKSMPIVIYITDDVHEDCDPDNNESYSVSNCVWR
                     VGDTLQNGHTFEEAITMMSSKGAKFIGIDTWNALNTDGSPTDASEPMDDMKMMAEITG
                     SLDKNGNPFIYHTTTPEGDGMPQQIGQAILDLTTFIDMDVTTGKLSDDQCDGISAAEF
                     IKSSTTVKAEPTTGISGQTSTTFQSVTQGTTVWFDVLFHNDFCKNTTTDPAVYDALVT
                     VLGNGSYLSNRLVKVIVPGAQAQ"
     gene            complement(7452..9257)
                     /locus_tag="PKH10_02140"
     CDS             complement(7452..9257)
                     /locus_tag="PKH10_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HNT26956.1"
                     /translation="MSEPEKKLTPEEEEQQWLETVYQGDKPQLTLRAIVTGSLLGGFM
                     SLTNIYIGLKTGWGFGVAITAVILSFAIWNVLSKVFHTPNMTLLENNIMQSLASASGY
                     TTGGTLVSAIAAYILVTGQHLNIWVLGFWILLIALLGTFMAIPLKRQMINVEKLPFPS
                     GTASAETLKSLYSSGVESARNAKLLFATAGIGIANKLIGEDLLGLVRYEYFVFGQWAQ
                     KFTMSIESSLLLVGGGALMGWRTAWSILFGAILNYAVVAPWLAGQGIIAPGEDGMFTY
                     RTIVGWSLWFGASVMVMAGLTAFAFQWRVIARTFVGMADIFRKQKRTLTAYEEIEVPG
                     SWFAAGMALTSAGIVIIMYFVFEVGILMSLVAIALSFVLAMVACRATGETDTTPVGAM
                     GKLTQLVYGILVPKRMIPNLMAASITADAAGASADLLTGLKCGYLLGANPRKQFLAQF
                     IGVFAGTLVVVPVFYIIVPGPEVLGTQMFPAPAAQIWASVAKLLSEGLDALSISIRWS
                     ILAGAIVGVVIPLIDRFLPKLSRFTPSAMGIGLAFTVPFAMCLSMFFGALIVLIISWK
                     KKDWEERIVPAASGLIAGESLAGVFLLIWSTLTNG"
     gene            complement(9270..9617)
                     /locus_tag="PKH10_02145"
     CDS             complement(9270..9617)
                     /locus_tag="PKH10_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26957.1"
                     /translation="MTKFLLMILSVLCANLLSGALSSLVLAKVRGSYSMAIATAVGML
                     VLVAVLYPLYRYLNSWATALSQFFLRTGKKMFGRILGLLLVFLALLALLYYGYARLWF
                     GVNIYAVLFGNFV"
     gene            complement(9696..>9937)
                     /locus_tag="PKH10_02150"
     CDS             complement(9696..>9937)
                     /locus_tag="PKH10_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT26958.1"
                     /translation="AHPRAEIVHLAIDPGKGLALHTMPMPVLFFVIEGEGTLLTADGA
                     ERYATGTLIECPVGIKRGWANEGTAPLKVLVIKLV"
BASE COUNT         2090 a         2801 c         3022 g         2024 t
ORIGIN      
        1 tgtcgaccga ggtcttctac gagatacaga agtacatgtt caattcgccc gatatcgcga
       61 aaaagatact ttcgcaccgc gacaccgaac tgaattggct caacggcgtg atggccgagt
      121 atctcgaaga atcgcccgac gacgaccgga cccgcaagat aacactgatg tacgccgcct
      181 ttctccattc gttcgtcacg atgtcggccg ggatagcgaa gcaggccaag gcgctcgaat
      241 cctattccat catatcgcag ttcccgtggc ggctcgacaa catcgaccac agcgacatct
      301 tcgagtttct cataggcggg gtggacgacc tgaagaagaa actgttcggt acacaagggt
      361 aaggagatcc ctatgaacac cagatcggta tcggtaatgc tcactctgat gacggcgtgc
      421 ggcttcttcc tgttcctgtc ctgttccggc aagccggagg gcaatggcgc cgaggcggtg
      481 caggcggtca ccatcaccaa gccggagaaa aagaagttca gtcgctcctt cgatacggcg
      541 gcggtcgcga tggccgagcg atcggcctat atcaatccca aggtcgcggc gatcatccgc
      601 tccttcaggg tcaaggaggg cgatttcgta aaggaaggcg cgctcctcgc gcaactcgac
      661 gggagtgact acgagatagc ggtgaatgcc tcgcgcgcgc aactcgcggc ggctgaagcg
      721 ggtgtcatgc aggccgaggc ggccttcgcc aagataagcg cagacttcga gcgcttcaag
      781 gcgctcaagc agaacggctc catatcggca agtgatttcg agcagatgga ggcgggatat
      841 aagcagaccg aagcgggact tgccgccgcc aaggcgcaac gcggcatggc gcagagcgcg
      901 ctcgacggga atctccggca actcggttat acggccctta tcgcgccgtt cacaggctat
      961 atcgcgacac gcaacggaga gatcggcgag atggccgcgc cggcgagccc ccggccgatg
     1021 ttcgagatcg tgcaaagcga cacgctcaag atacaggtct tcatcagcga actcgagatg
     1081 cgcggtgtca ccaaggatac ggtggcaaag gtctttttcg acgcttatcc cgacaaggag
     1141 gcttcggcga aggtgaatct gataaacagc aaggtcgatc cgatgaccaa gagcgtgaag
     1201 gtcgaactgg tcatggaaaa ccgcgaccat ttcttcaagc cggggatgac ggtgcgggtc
     1261 aggttcacgc tccccgaaaa agaatacctc gtggtcccgc gcaacgccgt tttcacccgc
     1321 gacaacgagg cgggcatcgt gtatgtcaag gatgccgatg accgcgtttt caccaaggag
     1381 atatttatgg gtggcgcggt cgaggggtac accatcgtcg aaaaagggct tgatggcgcc
     1441 gaagacatcg tggtcggcgg cggcaggcgg ctcgaagagg gacagaaggt cagggtggtg
     1501 acaccgggaa cggcggttgc cggagaggtc aggaaatgaa tcttccgaaa ttcgccgtca
     1561 aacgaccggt actggtatcg gtcatcttcg tgatgatggg gttgttcggc gtttattcgc
     1621 tgatggacct tcccatagat ctcatgcccg acatcgaggc ccccgccata tcggtcatca
     1681 ccatctaccg cggcgccggt tccgaagagg tcgaggagaa ggtaagccgc gtcatcgagt
     1741 ctgcgctcgc ctcgaccgcc ggcatgaaga acatctacag ccgttcgatg gagaatgtct
     1801 cgtcggtcac ctgcgagttc gagttcggtg tcgatcttgc cgaggcgacc aacaagatcc
     1861 gcgacctgct gaacatgtcg cgctccttcc ttcccgacga catcgatgag ccgctcgtct
     1921 tcaagttcga tttcagcatg atgcccatca tgttcctcgc gataacctcc tcgaaggagg
     1981 acatccgcta caagggcgag gaaatagata actatatcac gaattatttc gagcggatac
     2041 cgggtgtcgg ttcggtcatc accttcaacg agatgaagaa gaagatcatc atctcggccg
     2101 agaaacaacg gctttcggcg ctcaatctct cggtcgacga tctggttaac accatccggg
     2161 ccgagaacct gtcgcttccc gccgggaaca tcgagatcgg ttcctacgac tataccatac
     2221 gggtgcccgg cgaatacaaa agcatcgaag agataaacga gacgatcatc gccagcactc
     2281 ccgcggggct catccggata aaggatgtcg cgcgggtctt ctggggaagc gaggatagcc
     2341 gccagtacgg catcaaggac ggcgagtaca tggtcttcat gatggtccag aaacagtcgg
     2401 gggccaatac cgtctcgatc gccgatgcgg tgaaaaagga gatagagaag atccgtccgc
     2461 ggctccccga cgggtaccgg atcggcatcc tgctcgattc ttccgatttc atagtgaaga
     2521 tgatcaggaa cctttccacg gcggtcctca ccgcctgtct gttcgtcctt cttgtcgtcg
     2581 tctttttcct gcggcgattc cggtcgagtt tcatagtcct cctttcgata ccggcatcca
     2641 tgatcatcgc cttcgcgttc ctctacggtt tcggattcac gctgaacctc gtctcgctca
     2701 tgtcactgtc gctcgcgatc ggcatggtgg tcgacaactc gatcgtcgtg ctcgacaata
     2761 tcacccgtta cttggagtcg gggaaaagcc gggcggacgc agccatagaa gggacctccg
     2821 aggtcggcgg cgcgatcgtc gcctcgacgc tgaccaccat cgtcatcttc gcccccctct
     2881 ttttcgtggg tggactcatc ggcatactct tcggccaact tgccggcgtc atcgtgctga
     2941 ccctctccgc ctcgcttctg gcggcgatgc ttctgacgcc gatgatctct tcccgcatac
     3001 tcaccactca agggaacggc ggctcgtacg acaactggtt cttccggata ggggagcgct
     3061 ttctgacctt cgtcgaggat cgctacacgc gggtcatccg gctcactctg caacataaga
     3121 aaaagaccat tctcgtcgcc ggtctcacct tcctcgggtc gctgatgatc gtgccgctca
     3181 tcggggtcga cttcatgccg gaaggtgacg aggggctcat acagatcgaa tatgaactgc
     3241 cgctaggaag cagaatagaa gagacgctca aggtaggcca acacatcgag cgcataatcc
     3301 gcgaagaggt gccggagcgg tatctggaac gggtcttcct gcggggcggt cccgatgatt
     3361 ccggtttcgc ccagaaggtt caggacacca atacggggct taccggcgcg aaactggtgt
     3421 cgcagaactt ccggaaagag ggtatcaagt tcttcgccaa caagatccgg gcacgcatca
     3481 tcgccgaggt ccccggcatc gagaagatgg atgtcaagac ctccagcgga ttcggcgacc
     3541 tgatatcggg aggcgagaag ccggtgacgg tgaaactcgc ccataaggac tttaagaaac
     3601 tcaacgaggc agcggcccgg ctggaaaagg agttccagaa gatagcgggg ctcaaggaca
     3661 tcggcaatga cgccgattcg ctcaagccgc agatcgaggt gaagatagac cgcatccggg
     3721 cgagtcaggt ggggctcaag accgcgatga tagccgcggc ggtccgcaac gccttctacg
     3781 gcagcaccgc gtcggtgtac cgcaaggacg gcgacgagtt cgagatcatg gtcaagtaca
     3841 agaaggccga ccgcgagaac atcgaacaac tgaaagaact cgagataaag accatgtatg
     3901 gcacggtggt caaactcaag gatgtcgccg aggtcgcgga gggcctgacc tcgctgtcgg
     3961 tcaatcgtca gaaccgcgag cggatcgtgt cggtcggcgc gcggctcgag gaggatgtcg
     4021 ccatcggcaa gataggggcg gaggtcgaaa aggcgatcga acgggcgaac atagaccccg
     4081 agatcggcat catctacggc ggcacgctta aaagtcagaa ggagaacacc ggcgatctgg
     4141 tgcgcatgct cctgctcggc atactactcg tctatctggt catggcggct cagttcgaat
     4201 cgttcgtcga tccgttcgtc atcatcttct cgataccctt tgcgataagc ggcgtgctga
     4261 tcggcctgtt gctcacgggg cataccttgt cggttcccgc tttcctcggg atgatcatac
     4321 tcgtgggcat cgtcgtcaac aacgcgatag tgctcatcga ttacacgaag atacagcagg
     4381 aaaaacacag gttcggcacc gacgaatcgc tcatttattc cggcgctcgc cggttgcgcc
     4441 ccatcctcat gacggccacc accaccatct tcggcatgct gccgctcgcg ctggtgagcg
     4501 gcgaaggcca cgagacctac aatccgatgg gcgtcgcggt cgtcttcgga cttgcctttt
     4561 cgacgctggt gacgctcgtg ctcatcccct gcatgtattc gacgatcgac ggtttcttaa
     4621 gaaaacgcgg ctggcggacc gactaacggc ggtcgaacga cggttcagga actgaacggt
     4681 gcgaagaact gcacattcaa ctggtacacc gcatcgcgga ccggtcgttt tgcggcgata
     4741 agacgcaatt cctcctgaag cgcgatgacc cgttgcttca tctcctcgta gagttctttg
     4801 gagaccgcga acgacatgcc gtagaacaga cgcttctcga aaggttggtt gtcgagcgcc
     4861 tcgagcgctt tggtcatgcc gttgcgggtc tgtgcctttc gtatcgtgac gagttcatcg
     4921 ttcttctcgc gggccgcggc gacatagtcg gaaaggttta tccgttccgg gcgcagtttg
     4981 ccttgccggt cacgtgagat aaaaccatgc gcaagcagtt tgtcgagtcc ttcacggacc
     5041 gcggcgggcg ctatgccggt gaagaaattc tttgcgatcc acgacggatc ctcgcggaag
     5101 gtgtcgagtg cgaccacctc ccagagtatg gggagtttcc agtcgctgaa caacacgagc
     5161 gccgccgtca ggtaacggtc ccctgaaagg aaacgcgaag aggcgatcat ggcgcgaatg
     5221 atccgttcgc gttcctcctc ggtcttcgct ttctccagtg cgatcatggc ctgaagatgt
     5281 cctttctcct cctttgtcag accgagtgcg cgggcgtagc gttcggcgga tgtcagggag
     5341 agccgcacgg tgccgttgat cacctgccag aaatgattgg agcgatcgct gagccccgcg
     5401 ctacgctgga acgaacggat ggagaatcct ttctcctccc gtttcaactc ctcgtagacc
     5461 gaccggaaat aggtccggta ccgggtgtgt tgaaagatgt cggtggcggc ccttttcacc
     5521 gtgcgatctt ttttcggacg gctcattgcg cctgcgctcc cgggacgatg accttaacga
     5581 gtcggttgct gaggtacgag ccgttgccga gcaccgtcac aagcgcgtca tataccgccg
     5641 gatcggtcgt cgtgttcttg cagaaatcgt tgtggaacag gacatcgaac caaacggtgg
     5701 tcccctgagt gaccgattgg aaggtggtag aggtctgtcc cgaaatgccg gtcgtcggct
     5761 ccgccttgac cgtggtcgag gatttgatga actccgcggc gctgatgccg tcgcactggt
     5821 cgtcagaaag tttaccggtc gtcacatcca tgtctatgaa ggtggtcagg tcgagtatcg
     5881 cctgtccgat ctgttggggc atcccgtcac cttcgggggt ggtggtatga tagatgaaag
     5941 gattgccatt cttgtcgagc gaaccggtta tctcggccat catcttcatg tcgtccatcg
     6001 gctcggatgc gtcggtcggt gagccgtcgg tgttgagggc gttccaagtg tctatgccga
     6061 tgaacttcgc ccccttgctt gacatcatgg tgatggcctc ttcgaaggtg tgtccgttct
     6121 gtagggtatc gccgacgcgc cacacgcagt tagagaccga gtatgattca ttgttgtcgg
     6181 ggtcgcagtc ctcgtggaca tcgtcggtga tgtagatgac tatcggcatc gattttttcc
     6241 gcaggcaggc gtgcccgatg gaaccaagcg cgccggtgca gtcggccggc gggacatata
     6301 tctccgcctc ggcgatacag cccatcccgg gagggcaaag cgatgcgtgg ccgtggaact
     6361 gttcatcgga aaaactcagg tagagcggct cgcttgccat ttcgcttata ccgatgctga
     6421 acttctgatc ggccgggggg aggatattgt caagactcgc ctttatctca tcggcgtcga
     6481 gcgtcatctt ctggcgcagg aaattggccg tgccgtcgaa ggtgatgcgc gacagtccga
     6541 aggcggcgaa atcggggtcg gcatactgcg catttatttt gtcgatgata ttggtcttca
     6601 cctcttcttg tacccggtct atctcgccat acatcgaacc ggaggcatcg atgacgaaca
     6661 ggacatctat cgcctcgatg acggtgctga actcgagcgt ccggttggcc tcgtgcccgt
     6721 tgtagggaag cacgacgaag aagagaccgt ctggtatagt gtcggtatca tcggtcgggt
     6781 cggagccgta cacgatctcg gcaaggtcgt cggagccgtc atcgtccgag tccttcttgg
     6841 tcgggtcggt ccccgccgcc acttcatcct tatctgagag accgtcgccg tcggagtcct
     6901 tgtcgaggta gtcgggtttt tcgtcgccat cggtgtcgcg gcaaggttgt gaaggacact
     6961 ccgcgctgtc aagtataccg tcgccgtcgg cgtcggcgtc gagacagtcg ggggcgccgt
     7021 caaggtcggt gtcaacattc acctcgacac cattgggtat gccgtcgcca tcctcgtcgc
     7081 cgttcgggtc ggtgcagagt tcgttcaccg tgtcggtgtc ggggatgaga ccgtcgcttt
     7141 gcgggtcatc gctcgaggga tcatccggca ggatgcctcc gtctccctgt tcgttgtcgg
     7201 tcgaggcaag gccgtcgttg tcgatgtccg tgtcttcttc ctgcggggtg caggcggaac
     7261 agacgagcac tcccaaaaca gaggcgaaca acagggcgaa gatctttttc atggaaaacc
     7321 tccaatctgt gtagtggata tcgtcataaa catgaagata taactcgcct aaatataaag
     7381 caagaaaata acaaccattt ttttttactt aatattttta tttatttggt gatgagctag
     7441 cgtgtagttt tttagccgtt cgttaaggtc gaccagatga gaaggaagac ccccgcgaga
     7501 ctctcccccg cgatgagacc cgacgccgcc ggaacgatcc gctcctccca atccttcttc
     7561 ttccatgaga tgatgagcac gatgagtgcg ccgaagaaca tcgagaggca catggcaaag
     7621 gggaccgtga aggcgagccc gatacccatc gccgaggggg tgaagcgcga cagtttcggc
     7681 aggaagcggt cgatgagagg gatgacgacc ccgacgatgg cgccggcgag gatcgaccag
     7741 cgtatcgaga tgctgagggc gtcgagcccc tccgaaagca gtttggcgac cgatgcccat
     7801 atctgcgccg ccggcgccgg gaacatctgc gttccgagca cctcgggccc cggcacgatg
     7861 atgtagaaga ccggcaccac tacgagcgtc ccggcgaaga cgccgatgaa ctgtgcgagg
     7921 aactgtttgc gcggattggc gccgagcaga taaccgcact tgagccccgt cagcaggtcg
     7981 gccgatgccc ctgcggcgtc ggcggtgata gaggcggcca tcaggttggg gatcatccgt
     8041 ttggggacga ggatgccgta gacgagttgg gtcagtttcc ccatcgcccc gaccggtgtc
     8101 gtgtcggtct cgccggtggc gcggcaggcg accatcgcca gcacgaacga aagcgcgatc
     8161 gcgacgaggg acatcaggat accgacctcg aacacgaagt acatgatgat gacgatcccg
     8221 gccgaagtga gcgccatccc ggcggcgaac cacgaaccgg ggacctctat ctcctcgtag
     8281 gcggtcagtg tccgtttctg tttacggaat atgtcggcca tcccgacgaa ggtccgcgcg
     8341 atgacccgcc actggaacgc gaaggcggtg agtcccgcca tgaccatcac cgaggcgccg
     8401 aaccagagac tccagccgac gatggtccgg taggtgaaca tcccgtcctc gcccggggcg
     8461 atgatgccct gccccgcgag ccacggcgcc accaccgcgt aattgagtat cgcgccgaac
     8521 aggatgctcc atgcggtacg ccatcccatg agcgcaccgc caccgaccag cagcaggctc
     8581 gattctatcg acatcgtgaa tttctgcgcc cactgaccga agacgaaata ctcgtagcgg
     8641 accaggccga gcagatcctc gccgatgagt ttgttggcga tgccgatacc ggcggtcgcg
     8701 aagagcagtt tcgcgttccg ggcgctttcg acgccgctcg aatagagact tttcagcgtc
     8761 tcggccgagg cggtgccgct cgggaacggc agtttctcga cattgatcat ctggcgtttg
     8821 agcggtatcg ccatgaaggt gccgagcagc gcgatcagca gtatccagaa tccgagcacc
     8881 cagatgttga gatgctggcc ggtgacgagg atgtaggcgg cgatggccga caccagcgtg
     8941 ccgcccgtcg tgtagcccga cgccgaggcg agagactgca tgatattgtt ctcgaggagc
     9001 gtcatgttgg gggtgtggaa caccttggag aggacattcc agatcgcgaa ggagaggatg
     9061 accgccgtga tcgcgacgcc gaagccccac ccggtcttga ggccgatata gatgttggtc
     9121 agcgacatga agccgccgag gagcgatccg gtcacgatgg cacgcagggt cagttgcggt
     9181 ttatcgccct gatagaccgt ttcgagccat tgctgttcct cctcttcggg ggtcagtttt
     9241 ttttcaggtt cgctcatcgc atcctcccgt cagacaaagt tcccgaagag taccgcatat
     9301 atgttcacgc cgaaccagag tcgagcgtat ccgtagtata aaagcgccag aagcgcaagg
     9361 aaaacaagaa ggagtcccaa gatgcgaccg aacatctttt tgccggtcct gagaaagaac
     9421 tgcgacagag cggtcgccca cgagttgaga tagcggtaca gtggatagag caccgcgacg
     9481 agcacgagca tgccgacggc ggtcgctatc gccatcgagt acgaaccgcg taccttggcc
     9541 agtaccagcg acgatagtgc gcccgacagc aggttggcgc agagcaccga aaggatcatc
     9601 aggaggaatt tggtcatggg gcgactccgt tgagatgaac ggagggtcta tagcacgggg
     9661 cgtcggatat ttcaaattat aaaggggagg aggggctaga ccagtttgat gacgagcact
     9721 ttgagcggtg cggtcccttc gttggcccag ccgcgtttga tgccgacggg gcattcgatg
     9781 agcgtgcccg tcgcgtagcg ttcggcgccg tcggcggtga ggagcgtccc ttccccctcg
     9841 atgacgaaga agaggaccgg catcggcatg gtgtggagcg cgagtccttt gcccgggtcg
     9901 atggcgagat ggacgatctc ggcccgtgga tgggccg
//
LOCUS       DAOQUE010000039         9737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_7820_length_9737_cov_4.046788, whole genome shotgun sequence.
ACCESSION   DAOQUE010000039 DAOQUE010000000
VERSION     DAOQUE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7820_length_9737_cov_4.046788"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..345
                     /locus_tag="PKH10_02155"
     CDS             52..345
                     /locus_tag="PKH10_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26959.1"
                     /translation="MYKCFNCNKALDELDDIRGKVGRQDTCPHCYADLHCCMNCRFHE
                     PGYPNECRETSTAFIRERDKQNFCAAFEFKHAEEGDDNEELDAKAKLNSLFKL"
     gene            382..1107
                     /locus_tag="PKH10_02160"
     CDS             382..1107
                     /locus_tag="PKH10_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM-associated putative lipoprotein"
                     /protein_id="HNT26960.1"
                     /translation="MTKRFYKLMCFATAAMMSIIGCNKTTSGDNDQYQENEFKDTLYG
                     CESIYYDEADVDQPNIPDIDEDHYYSDTVYGDPGVDFDDSVDNDANVDEDVDDHSVIT
                     PDVDEPDVQGDEYACPYATFHVDGKAANTNGDPIRGVKVTAQYHFSSYPKTTATKSDG
                     SFLIESEEWTSFSDTEYVLLNFEDIDGELNGGLYVTQELNIQLDHLAGSGGWDWGTYE
                     KKNLFVTLYRVGETDDDTLLSDD"
     gene            1201..1566
                     /locus_tag="PKH10_02165"
     CDS             1201..1566
                     /locus_tag="PKH10_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26961.1"
                     /translation="MTKRFYKLMCFATAAAMSIIGCGPEDDPTVEYGAPTDLDQYQTD
                     NDEYTDATKYGVLDADYAPTDDMLPDADEPLVIPDEPAMEYGPVQTDYDDLKSDAIIE
                     QPDEMTTDYGPISRPFVKK"
     gene            1563..2660
                     /locus_tag="PKH10_02170"
     CDS             1563..2660
                     /locus_tag="PKH10_02170"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT26962.1"
                     /translation="MSIKRVAKRALLPLKQHYERRLHELRYIFFELTHRCNLACIHCG
                     SDCQKNDTIPDLPTEKILETLDGIKTKYDSHKVMIVLSGGEPTIYPGVWDLGKEIIKR
                     EFPWGMVTNGFAWDADMIKKAKEARMHSVTVSLDGLEETHNWLRGDKKSFRKALHAIE
                     LLLNDPFYRVMDVITCVNKRNIGELDKIYDLVKSMGLKRWRFFTISPIGRAVQTPDLF
                     LDKEQFHYLMQKILAYRRRPGMKVTYSESGYLGCYEHRVRGHDWFCRAGVNVAGIMVN
                     GDILACPNIDRRFKQGNVFEQPFIDVWEHGYKEFRDRKWMRTGDCVKCSEWRLCQGNP
                     MHLWDLDANQTKLCHLKQFELDKPLRGPDLD"
     gene            complement(2657..2923)
                     /locus_tag="PKH10_02175"
     CDS             complement(2657..2923)
                     /locus_tag="PKH10_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019021722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNT26963.1"
                     /translation="MTYRLAFKRSAAKELAKLDRTVQVIIKEKLELLCADPATLAPNI
                     KRLTGSGLFRLRVADYRVVFTKDDERIVILVVRIGHRKEVYLER"
     gene            complement(2920..3138)
                     /locus_tag="PKH10_02180"
     CDS             complement(2920..3138)
                     /locus_tag="PKH10_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26964.1"
                     /translation="MGRERATFTVDAEILRELESVAKELETTRSGLVEKSLLYFFDFL
                     DVRLAEKRLADKKDEVVPAEKVWEELGL"
     gene            complement(3215..3409)
                     /locus_tag="PKH10_02185"
     CDS             complement(3215..3409)
                     /locus_tag="PKH10_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26965.1"
                     /translation="MLRHFYRLMVVALGFFLASSCNRGSTDYGVIDTDYTSPDEDVVD
                     EDVAIDDDVANDDDTLVNDE"
     gene            complement(3438..4154)
                     /locus_tag="PKH10_02190"
     CDS             complement(3438..4154)
                     /locus_tag="PKH10_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26966.1"
                     /translation="MGPTADPGMAPEENERYRYLLFNPALWGLILSLVCSLYLAYGLF
                     KEPAAIENKYNEKQLEAYRSWGLKSPLEEDAEKQRKERVKAISGLAAFAFVGIFMVVQ
                     LTRRKTTLRQMFMPFHELVFDRSGLWYDRTLFLPWRLVVDFHYHDPSEDIERGGRRGK
                     EITISYIDPEHQSMNRLVISGWTPKFAQMKNYLEDVKNAAMPKNLQCPRCQKDFEVDA
                     QENKIIYSCRACGQQYTQEL"
     gene            complement(4171..4734)
                     /locus_tag="PKH10_02195"
     CDS             complement(4171..4734)
                     /locus_tag="PKH10_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM-associated putative lipoprotein"
                     /protein_id="HNT26967.1"
                     /translation="MFKHFYRLMVVALGFFVASSCGEATPKYGNIYTDYDDGTVDEDV
                     AESEAITPDEDVDNTLYGCIGCEFDVQGTVLNEQGVPIPNIEAIMYAGTSIAASTRTD
                     QNGAFSLVLKSNYGCDIGDPETVPLTFNDTDGSANGGNFESKKVDVVLPWDYNNGYGW
                     DPCAYKKSDIEVKLDKVGNDDDTLLKD"
     gene            complement(4752..5825)
                     /locus_tag="PKH10_02200"
     CDS             complement(4752..5825)
                     /locus_tag="PKH10_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26968.1"
                     /translation="MFKHFYRLMVVALAALFVTLTMACAGGRVINNDLDTVDHGVADE
                     DVFDEDIADSVDMDVSDLDVNDPDVVKPDDDIDADSSACDNGNIGKNCKTDNECGSCL
                     ICVNAKCAEGCQSDADCLNYAGTKCNRALSRCLNTTATDSACGETKCSSGCCYGTKGF
                     LSIKCLSTATLQTCGICKQGEIYMDGKQCVPSACKVGESKCQTYNATVPNSQCFSCDE
                     ETFLCQSDPNCAGADVCEGCEFGIQGTVMNAQGDTLTGIEVVAYAGTSFETSVRTGAT
                     GIFKLDLQSNYGCDIGEPETIPLIFNDTDGVANGGNFESKQVDAVLPWTESLVNPCTY
                     QKMDLVIALDEVGNNDDILFPDE"
     gene            5977..8691
                     /gene="secA"
                     /locus_tag="PKH10_02205"
     CDS             5977..8691
                     /gene="secA"
                     /locus_tag="PKH10_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNT26969.1"
                     /translation="MFKAIARKIFGSENDRFLKMCRPFVVRTNELEERVSKLTRGDMV
                     GRMNELRQMVANGEALDTLIPEVFAMTREASKRTLNMRHYDVQLVGGIVLHKGKIAEM
                     KTGEGKTLVATLALVLNALSGKGCHLVTVNDYLAQRDAEWMGNIYNYLGLSVGVVLHG
                     LSNADKKNNYNCDITYGTNSEFGFDYLRDNMKYDLANYVQRDLNFGIVDEVDSILIDE
                     ARTPLIISGPSEDSTELYSVVNSVMYHLKETTHFTRDEKAKSAVLTEDGVRKVEQLLN
                     VQNLYSPDNLQLVHHVQQALKAHFMFAKEVDYIVRENRVMIVDEFTGRIMEGRRWSDG
                     LHQAIEAKEGVKVESENQTLATITYQNYFRMYNKLAGMTGTADTEAAEFADIYNLGVI
                     VIPTNRPVIRKDHGDVIFKTRRSKEEQVLNEIRKRNEKGQPILVGTISVESSEVYRQL
                     LEKARIPHNVLNAKFHHKEADIIAQAGRKGAVTIATNMAGRGTDIVLGGNPEKLALAE
                     INGWLAGREATTPEERKAKFDELVEKYRPICIREREEVVAAGGLHIVGTERHESRRID
                     NQLRGRSGRQGDPGSSRFFLSLEDDLLRIFGGEKVFNIMDKLGLEEGQPIEHRWITKA
                     IENAQKKVEGHNFSIRKHVLEYDDVMNQQRKAVYALRRDILTGGEKNRETVFSMIEEV
                     TNALVGSSIPEGQSPGAWDCEAVREQLARVVMMAPENFTFVTPEVAANVPRGTDPRTM
                     LAQVIYDFLTGKYIEKMSQYTPELTFELERHIILEVLDFFWRRHLLTMDHLREGIGLR
                     GYGQKNPKLEYKREGFQLFTEMMSTIYSESVKRLFAVQIVTQESLAKFEKKEEKRERE
                     MQAGSAAKAAAVKEPAKRTAPKVNRNDPCPCGSGQKFKKCCMGKGIYD"
     gene            8691..9431
                     /locus_tag="PKH10_02210"
     CDS             8691..9431
                     /locus_tag="PKH10_02210"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNT26970.1"
                     /translation="MTGNVRINRFLAEAGVASRRKADILVAVGRVRINGDRAATGDKV
                     DPARDEVRVDDEPVARIAEKVWFLLNKPAGYLSAVTDDRGRHTVVELLPPTPGLFPVG
                     RLDREVEGVLLLTTDGDTAHRLTHPSFGITKVYRVLVAGNFPDTVIRALKSGVDIAGH
                     LCRIIDAVAVASDQTTTTLELTLAEGRKHEVKELIKAVDRLLLRLERISFAGLTAQGL
                     KPGEYRPLTKAEIGSIKKLTAQEPKDTK"
BASE COUNT         2299 a         2952 c         2595 g         1891 t
ORIGIN      
        1 ccaaaccccg tatgatgctt cgggtatgtt gtctgaaaga gggagacgcg tatgtacaag
       61 tgcttcaact gcaacaaggc gctcgatgaa ctcgacgata tccgcggcaa ggtcggtcgg
      121 caggacacct gcccccactg ttacgccgac ctccactgct gtatgaactg ccggttccac
      181 gaaccgggct acccgaacga gtgccgcgag acctcgaccg ccttcatccg cgaacgggac
      241 aagcagaatt tctgcgccgc gttcgaattc aagcacgccg aggagggtga tgacaacgag
      301 gaactcgacg caaaggcgaa actcaacagc ctgttcaaat tgtagcgttt cgaccgcgat
      361 aacgggggaa gggagccggt catgacaaag cgtttttaca aactcatgtg cttcgcgacg
      421 gcggcaatga tgtccatcat cgggtgcaat aagaccactt ccggcgacaa tgaccagtat
      481 caggaaaatg aatttaagga caccctctac ggctgcgaat caatctacta cgacgaggcg
      541 gatgtcgacc agccaaatat ccccgacatc gatgaagacc actattactc cgacacggtg
      601 tacggtgacc ccggcgtcga ctttgacgac tcggtcgaca acgatgcgaa tgtcgacgaa
      661 gatgtcgatg atcattctgt tatcactcct gatgtagacg aacccgatgt gcaaggcgac
      721 gagtacgcat gcccctatgc gaccttccat gttgacggca aggccgccaa taccaatggc
      781 gatcccattc gcggtgtcaa agtgacagcg caatatcatt tctcttcgta cccaaagaca
      841 acggccacta aaagtgatgg ctcctttttg atcgaatccg aagagtggac gtctttcagc
      901 gatacggaat atgtcctcct taatttcgag gacattgatg gggaacttaa tggcgggctg
      961 tatgtcaccc aagaactgaa cattcaactg gatcatctag cgggttccgg aggatgggat
     1021 tggggcacct acgaaaaaaa gaacctcttt gtcacgctct acagagtcgg cgagaccgat
     1081 gacgatacac ttctttccga cgactgaaac ataccagaaa aactggaaaa atcgacgcga
     1141 taggataagg ttgtcctgtt cttgccacta aaagggcgct catcggaaag ggagccggtc
     1201 atgactaagc gtttttacaa actcatgtgc ttcgcgacgg cggcggcgat gtccatcatc
     1261 ggttgcggtc ccgaggacga tccgacggtc gaatacggtg cgccgactga tctggatcag
     1321 taccagaccg acaacgacga atacaccgac gcgaccaaat acggcgtcct tgatgccgat
     1381 tatgccccga ccgacgatat gctccccgat gccgacgaac cgctggtcat ccccgacgaa
     1441 ccggcgatgg aatacggccc ggtgcaaacc gactatgacg atctcaaaag cgatgctatc
     1501 atcgaacagc ccgacgagat gaccaccgac tacggcccga tatcgcggcc cttcgtcaag
     1561 aaatgagtat caaacgcgtc gccaaacggg cgctcctgcc gctcaaacag cattacgagc
     1621 gccgtctcca cgaactgcgc tacatcttct tcgaactgac ccaccgctgt aacctcgcct
     1681 gcatccactg cggcagtgac tgccagaaga acgacaccat ccccgacctg ccgacggaga
     1741 agatactcga gacgctcgac ggcatcaaga ccaagtacga tagccacaag gtgatgatcg
     1801 tcctgtcggg cggcgagccg acgatctacc ccggcgtctg ggatctcggc aaggagatca
     1861 tcaagcgcga attcccgtgg gggatggtga cgaacggctt tgcgtgggac gccgacatga
     1921 taaaaaaggc gaaagaggcg cggatgcatt cggtcaccgt gtcgctcgac ggccttgaag
     1981 agacccacaa ctggctgcgc ggcgacaaaa aatcgttccg caaggcgctc cacgcgatag
     2041 aactgctcct caacgacccc ttctaccgcg tgatggatgt catcacctgc gtcaacaaac
     2101 ggaatatcgg cgaactcgac aagatatacg accttgtgaa gtcgatggga ctcaagcggt
     2161 ggcgtttctt caccatctcc cccatcggcc gcgcggtgca gactcccgat ctcttcctcg
     2221 acaaggagca gttccattac ctcatgcaga agatactcgc ctaccgccgc cggcccggga
     2281 tgaaggtcac ctactccgaa tcgggctatc tgggctgtta cgaacaccgc gtccgcggcc
     2341 acgactggtt ctgccgcgcc ggggtgaatg tcgccggcat catggtcaac ggcgacatac
     2401 tcgcctgccc caatatcgac cgccgtttca aacaggggaa tgtcttcgaa cagccgttca
     2461 tcgatgtctg ggagcacggc tacaaagagt tccgcgaccg gaaatggatg cggaccggcg
     2521 actgcgtgaa gtgctccgaa tggcgtttgt gtcagggaaa tcctatgcac ctgtgggatc
     2581 tggacgccaa ccagacgaag ctctgccatc tgaagcagtt cgaactcgac aagccgctcc
     2641 gcggacccga cctcgactag cgttccagat agacctcttt gcgatggccg atgcgcacga
     2701 cgagtatcac gatgcgttca tcgtctttgg tgaaaacgac ccgatagtcg gccacccgca
     2761 gacggaaaag cccactgccg gtcaaccgtt tgatgttcgg cgccagcgtc gccggatcgg
     2821 cgcacaggag ctccagtttc tctttaatga tgacctgcac ggtgcgatcg agtttcgcca
     2881 gttctttcgc cgccgaccgt ttgaatgcga gccggtaggt cacagaccca attcctccca
     2941 gaccttttcg gcgggaacca cttcatcctt cttgtcggcg agtcgtttct cggcaagacg
     3001 cacatcgaga aagtcgaaga agtaaagaag cgatttttcg accagcccgc tccgcgtcgt
     3061 ctcaagttct tttgccaccg attccagttc gcgcagtatc tcggcatcca ccgtgaatgt
     3121 cgcccgttcg cgtcccatga gaacctccgt cacacaaaag tgaccataat catacaaatg
     3181 attatatgaa cgaatgcgcg catgtcaagg gaaattattc gtcgttgacg agcgtatcgt
     3241 catcgttggc aacatcgtca tcgattgcga catcctcatc gacgacatcc tcgtcgggcg
     3301 aagtataatc agtgtctata acgccataat cagtcgatcc tctgttgcaa ctcgacgcga
     3361 ggaagaaccc cagcgccacg accatgagcc ggtaaaagtg cctgagcatc cctctctccc
     3421 ccaccgatag actcaatcta caattcctgt gtatactgtt gcccgcaagc acgacatgaa
     3481 taaattatct tattttcctg cgcatcgacc tcaaaatcct tctgacatcg ggggcactga
     3541 agattcttag gcatggcggc atttttcaca tcttcaagat aattcttcat ttgagcgaat
     3601 ttcggcgtcc atcccgaaat gaccaaccga ttcatcgact ggtgttccgg gtctatgtaa
     3661 gatattgtga tttcttttcc ccggcgtccc cctctctcta tgtcttcact cggatcatgg
     3721 taatgaaaat ccaccaccag ccgccacgga agaaacaaag tgcggtcata ccataaaccg
     3781 gaacggtcaa aaaccaactc atgaaaaggc atgaacattt gtcgaagggt ggtctttctg
     3841 cgggtgagct gcaccaccat aaagatgccg acaaaagcga acgcggccaa gccactaatt
     3901 gccttcaccc gttctttccg ctgtttttcg gcatcttctt ctaggggact tttcaatccc
     3961 cacgaccggt atgcttccaa ctgtttctca ttgtacttgt tctctatcgc ggccggttcc
     4021 ttgaagagcc cataggcaag atagagagaa caaaccagcg acagaataag gccccagagc
     4081 gccgggttga aaaggagata tcgataacgc tcgttttctt ccggcgccat tcccggatca
     4141 gcagtcggtc ccattagaca gcctcttttt tcaatccttc agcagggtat cgtcgtcgtt
     4201 accgaccttg tcgagtttga cctcgatatc gctctttttg taggcacagg gatcccagcc
     4261 atagccatta ttgtagtccc acggcaacac gacatccacc ttcttactct cgaaattgcc
     4321 cccgttcgca gaaccgtcgg tgtcgttgaa ggttaacggc accgtttccg ggtcgccgat
     4381 atcgcatccg tagttgcttt tcagaacaag actgaatgcg ccattctgat cagttctcgt
     4441 gctcgcggct atagatgtac cagcatacat gatagcctcg atattgggaa tgggcacccc
     4501 ctgctcgttt aaaacggtcc cctgcacatc aaattcgcat ccgatgcaac cgtaaagagt
     4561 gttgtctaca tcctcgtcgg gcgttatggc ttcgctttcg gcaacatcct catcgaccgt
     4621 gccgtcatca taatcggtgt aaatattgcc gtacttcggc gtggcctcgc cgcaactcga
     4681 cgcgacgaag aacccaagcg ccacgaccat gagccgatag aagtgtttga acatgcacgc
     4741 ctccccctcg cttactcatc cgggaacagg atatcgtcgt tgttgccgac ctcgtcaagc
     4801 gcgatgacaa ggtccatctt ctggtatgtg caggggttta ccaaagactc tgtccacggc
     4861 agtacggcat ccacctgctt actttcgaag ttgccaccat tcgcgacgcc gtcggtgtca
     4921 ttgaaaatta acgggatcgt ctccggttca ccgatatcgc atccgtagtt ggactgaaga
     4981 tctaacttaa aaatgccggt cgcccccgtc ctcacgcttg tttcaaagga ggtccccgca
     5041 tacgcgacca cttcgatacc ggtcaaggtg tcgccctgcg cattcatgac ggtcccttgg
     5101 ataccgaatt cgcatccctc gcaaacatcc gcgccggcac agttcgggtc gctctgacag
     5161 aggaaggtct cctcatcgca cgagaaacat tgggaattcg ggacagtggc gttgtatgtc
     5221 tggcatttgc tctcgcccac cttgcaggcg gacggaacgc actgcttgcc gtccatgtaa
     5281 atctcaccct gcttgcagat gccgcaggtc tggagcgtcg ccgtcgaaag acatttgatc
     5341 gacaggaatc ccttcgtgcc ataacaacaa ccggatgaac attttgtctc gccgcaggcg
     5401 ctgtcggtgg cggtcgtgtt caggcaacgg ctgagggccc tgttgcattt tgttccggcg
     5461 tagttcaggc agtcggcatc ggactgacaa ccttcggcac acttggcgtt gacgcagatg
     5521 aggcaggagc cacactcatt gtcggtctta cagtttttac cgatgttccc gttgtcacag
     5581 gcgctactgt cggcatcgat atcgtcatcg ggcttcacga catcaggatc gttgacatca
     5641 aggtcgctca catccatatc gacgctatcg gctatatctt cgtcaaacac atcttcatcc
     5701 gcgacaccgt gatcgaccgt atcgagatcg ttattgatga cgcgcccccc cgcacaagcc
     5761 atcgtcaaag tgacgaacaa cgccgccagc gccacgacca tgagccgata gaagtgtttg
     5821 aacatgcacg ccctccctcg ccgacgaccg cggcgaaaac cgttcacata gtatcagacc
     5881 ggaaatttaa gtcaaccgtt cctccttttt acctctaaaa ttgctttttc cccatttttt
     5941 ggttacctgt gctgaacatt ttcttgggag tacgccatgt tcaaagccat agcacgaaag
     6001 atattcggga gcgagaacga ccggttcctc aagatgtgcc gccccttcgt cgtccggacg
     6061 aacgaactgg aggagcgggt ctcgaaactg acccgcggcg acatggtggg tcgcatgaac
     6121 gaactgcgcc agatggtcgc gaacggcgag gcgctcgaca ccctcatccc cgaggtcttc
     6181 gcgatgaccc gcgaggcgag caagcggacg ctcaacatgc gccactacga tgtgcaactc
     6241 gtcggcggca tcgtcctgca caaagggaag atcgccgaga tgaagacggg cgaaggcaag
     6301 acgctggtcg ccacgctcgc actcgttctc aacgcgctgt cgggcaaggg ctgtcatctg
     6361 gtcacggtca acgactatct cgcccagcgc gacgccgaat ggatgggcaa catctacaac
     6421 tacctcggcc tgtcggtcgg cgtggtgctc cacgggctgt cgaacgccga caagaagaac
     6481 aactacaact gcgacatcac ctacggcacg aactccgagt tcggtttcga ttacctgcgc
     6541 gacaacatga agtacgatct ggccaactat gtccagcgcg acctcaattt cggcatcgtc
     6601 gacgaggtgg acagcatact catcgacgag gcgcgcaccc cgctgatcat cagcggcccg
     6661 tcggaggact cgaccgaact ctattccgtg gtcaattcgg tcatgtacca cctcaaggag
     6721 acgacccatt tcacccgcga cgaaaaggcg aaaagcgcgg tgctgaccga ggatggcgtc
     6781 cgcaaggtcg aacaactgct gaatgtgcag aatctctatt cgcccgacaa cctgcaactg
     6841 gtccaccatg tccagcaggc cttgaaggcg cacttcatgt tcgccaagga ggtcgactat
     6901 atcgtccgcg aaaaccgcgt catgatcgtc gacgagttca ccggtcgcat catggagggg
     6961 cgccgctggt ccgacggact ccatcaggcg atcgaggcaa aggagggggt gaaggtcgaa
     7021 agcgagaacc agacgctcgc caccatcacc taccagaact acttccgcat gtacaacaaa
     7081 ctcgccggca tgaccggcac cgccgacacc gaggcggccg agttcgccga tatctacaac
     7141 ctcggcgtca tcgtgatacc gaccaaccgc cccgtcatcc gcaaggacca cggcgatgtc
     7201 atcttcaaga cccgccggtc gaaggaggaa caggtcctca acgagatacg gaaacgcaac
     7261 gaaaagggcc agccgatact cgtcggcacc atatcggtcg agagttccga ggtctaccgg
     7321 caactgctcg aaaaggcgcg aataccgcac aatgtcctca acgcgaagtt ccaccacaag
     7381 gaggccgaca tcatcgcgca ggcgggccgc aagggcgccg tcaccatcgc caccaacatg
     7441 gcgggccgcg gcaccgacat cgtgctgggc ggcaaccccg agaaactggc gctcgccgag
     7501 atcaacggct ggctggcggg gcgcgaggcg accaccccgg aggaacggaa agcgaagttc
     7561 gacgaactgg tcgaaaaata ccgccccatc tgcatccgcg aacgggaaga ggtggtcgcg
     7621 gcgggcggcc tgcacatcgt cggcaccgaa cggcacgaat cgcgccgcat cgacaaccag
     7681 ttgcgcggcc ggtcgggccg tcagggcgac cccggctcgt cccgcttctt cctgtcgctc
     7741 gaggacgacc tgttgcgcat cttcggcggc gagaaagtat tcaacataat ggataaactc
     7801 ggtctcgaag agggacaacc gatagagcac cgctggataa ccaaggcgat agagaacgcc
     7861 cagaagaagg tcgaagggca caatttcagc atacgcaagc atgttcttga gtacgacgat
     7921 gtgatgaacc aacagcgcaa ggccgtctac gccctgcgcc gcgacatact caccggcggc
     7981 gagaagaacc gcgagacggt gttctcgatg atcgaggagg tgaccaacgc gctcgtcggc
     8041 agctccatcc ccgaagggca atcgcccggc gcttgggact gcgaagcggt ccgcgaacaa
     8101 ctcgcccggg tcgtgatgat ggcgcccgag aacttcacct tcgtaacccc cgaagtggcg
     8161 gcgaatgtcc cgcgcggcac cgatccccgc accatgctgg cgcaggtcat ctacgacttc
     8221 ctcaccggga aatacatcga aaagatgtcc cagtacaccc ccgaactcac ctttgaactc
     8281 gaacggcaca tcatcctcga ggtactcgac ttcttctggc ggcgccacct cctgacgatg
     8341 gatcaccttc gcgagggcat cggtctgcgc ggctacggcc agaaaaaccc gaaactcgaa
     8401 tacaagcgcg aaggcttcca actcttcacc gagatgatgt ccaccatcta cagcgaatcg
     8461 gtgaaaaggc ttttcgcggt ccagatcgtg acgcaggaat cgctcgcgaa attcgagaag
     8521 aaggaggaga aacgcgaacg agagatgcag gcggggagcg ccgcgaaggc ggcggcggtc
     8581 aaggaaccgg ccaagcggac cgcgccgaag gtgaaccgca acgacccgtg cccgtgcggc
     8641 tccggtcaga aattcaagaa atgttgcatg ggaaagggca tctacgactg atgacgggga
     8701 atgtccgtat caaccgcttt ctggccgagg ccggggtcgc ctcgcgtcgc aaggccgata
     8761 ttctcgtagc ggtaggccgc gtccgtatca acggtgaccg ggcggcgacc ggcgacaagg
     8821 tcgacccggc gcgcgacgag gtccgggtgg acgacgaacc ggtcgcccgc atcgccgaaa
     8881 aggtctggtt cctcctcaac aaaccggcgg ggtacctctc ggccgtcacc gacgaccgcg
     8941 gtcgtcacac cgtcgtcgaa ctactccccc ccaccccggg cctcttcccg gtcgggcggc
     9001 tcgaccgcga ggtcgagggg gtactgctcc tcaccaccga cggcgacacc gcccaccggc
     9061 tcacccaccc cagtttcggc atcacgaagg tctaccgcgt ccttgtggcg ggcaattttc
     9121 ccgataccgt catccgcgcg ctgaaaagcg gggtcgatat cgcgggccac ctctgccgca
     9181 tcatcgacgc cgtcgcggtc gccagcgacc agaccaccac gacgctcgaa ctgacgctcg
     9241 ccgaagggcg caaacacgag gtcaaggaac tcatcaaggc ggtcgaccgc ctgctcctgc
     9301 gcctcgaacg catatccttc gcggggctga ccgcgcaggg actcaaaccg ggcgagtacc
     9361 gcccgctcac caaggccgag atagggtcaa taaaaaaact gaccgcgcaa gaaccgaaag
     9421 atacgaaatg aaccggctcg cagttagagg acagaacaag gcgcaagccc cgcaggcaac
     9481 gaagctgtac tacccgtaca gcgaggccgt cgaggagcgg caacgaagtt atgttcctaa
     9541 atgcgagccg gataaaaatg aagcccgcgc cggccgtgta tccgactcgt ttcttacctg
     9601 tgcgtcacag gtggggcatg ttcggccgct acctttttac agtcacggct cggtgacggc
     9661 cccgaaacgt agtcacggta gaaactacat tcggggccgt caccgagccg tgactgtaaa
     9721 aaggtagcgg ccgaaca
//
LOCUS       DAOQUE010000040         9728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_7827_length_9728_cov_4.071539, whole genome shotgun sequence.
ACCESSION   DAOQUE010000040 DAOQUE010000000
VERSION     DAOQUE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9728
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7827_length_9728_cov_4.071539"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4169
                     /locus_tag="PKH10_02215"
     CDS             <1..4169
                     /locus_tag="PKH10_02215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT26971.1"
                     /translation="LSPEQCQTNTIDHRSDLYALGLIFHEALAGEPPFAEPPGTLAPA
                     ALSPDAIVFPEAFDLDMARIIKGLLQPDPAERTPDALTLLNELESLESKLIMARAAKR
                     PARALPLRIHEPEFIARRAEMARLVTAIDNFLSSGLDMTVIVEGERGAGKTRFLEEVR
                     ARMAFRDLSTVLVSAADTQSLASDLLKGLWDTADRELRYQIAVKWGALIRTYFPECAV
                     APEFRRITVPAAEEHSMEENLHRVVACIGDIIETITRTRPLLLFIDNFGDIDKRSLAV
                     MQELYAHAENYRRVFTTLAVTTENAVSPIEFPANARVTIPSFSPGELRDFIESCLQSP
                     RKHIDESLFLWLYRMSGGIVKTAFSLLHMLREEGFLLLSGERILFRNDVLEKEPIDFL
                     FRLKLNSLSSDQRALLNLAAIYRKFTTREALSAVLLERMSDEAFRDALDFLKRNYLLE
                     EHKNGKIRLVNARLQALVYQSMPADERRRLHLSHADYLLTSNPLEEINTCAFAAYHYL
                     RGGRPETALKYYLRSASRAFFHLNTELTGSFIDEALSIVRENPYILSLKKKTAVSLFA
                     GRIFHRLGIYGRAIPLLEEAYGTWKDELILDLLVRALVGDGDAPKALQHTVKFKADTP
                     ERAAFVAYLRAYIAGRAEENYAKAASHIQKAKRLMAAGHDALFTPQRRYLLRELEFDH
                     AMYERGSDYETLLTVRNDLIESAKKLPRKAYLIDALTASFRFLQRFNKLEKGYLLLQQ
                     TLKLAIESFDNFRIARAYLNLASCAQLLERWHEVPFFLDRAMDFARKGCGTTMLKYAF
                     FARGEFAVLTGDFSLAENYLFNAEELSYRERRDSDLIGIYAAQVLLYLLKGESGFARV
                     AGGKLRRFLDKKKLDDQRRCRALCTLLFLEASFEGDREYILELSDQIEALFKQHPELR
                     PLYRLLYLTARALYAAQKGYDRDLTRIVETIEDEKVSAVAPLFRLLYRHRIAHILSEK
                     KALPEILRRLIDEGRQESALLQARHFITLFGELSYFIDYGQTDQLMAEVRAAIDEAAS
                     GDRSERTLGRLKLYFDETRRQTDFLRTRNRNYSYIIDIVKAISGKTEVGKIMDIVVKK
                     ILDILSADLVAVVFQYDDGRQMDYLIKDAAQQTYKFNDIRFKGDIVQRMFKTAKIEFF
                     TSMPLSFSDTDSSASSGPDADNCVVAAIPVLLQGEIRAYLYLERNLSKGAFLEEEIQF
                     LEILADNIGVIFDNVKLVEIATTDALTRVATRRHFLSILKKECEKARRYGFTLSLIMI
                     DIDLFKNINDTYGHLMGDTVLRQIGRLLKNNIRNSDYAGRIGGEEFALLLSGTNATGA
                     FNTAEKIREKCQRSSFSGLQLTLSTGIASYHEHEVKNEEDLLQKADTALYAAKRRGRN
                     MTVRYDDREG"
     gene            4169..5338
                     /locus_tag="PKH10_02220"
     CDS             4169..5338
                     /locus_tag="PKH10_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26972.1"
                     /translation="MPVTIFVEENSIVAGNTDKVRRTLEPIVENSDMVRRYEAGLALS
                     FGPAPGKRPLGFFDLEKRLSQPSYKHWFRRMDREVPALPYFLAADDPGKSLLIYLMGV
                     LDYKLIGERVTFDAAAMDVFFHHKKTEIKNLCLSNNISPLASIQRLADLLAPAARSDG
                     PVATPPPAAAPPAKEERPDTDRPAEAQLLKQLLDRFGSIAVLKENRMTKLFVVFDEIP
                     PQIRLVRNELVLDRSLNRWFFRTVFLIDGRESARESLLLYRPNEVTESIQFFNGALIM
                     CVYRDETSEYQKLFETDEPVRTDIVEQAGHAVAEVSGGQAAPDGPADEGTTGQASPPP
                     AAAPEPAPQAAPADPKDARIAELEAEVAKLKNIVEAYEETLLGKNKKSIFKKFFG"
     gene            5335..6096
                     /locus_tag="PKH10_02225"
     CDS             5335..6096
                     /locus_tag="PKH10_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT26973.1"
                     /translation="MMRVTLLGTGSSTGTPQLLCDCENCRSTDPRDRRTRFAILLEQG
                     DTVLLVDAPFEIRLQLLAAKVKRLDALWLTHAHSDHLAGVDDLRIFAFRNGAPIPCFA
                     LSETIATTRHRFTYLFDDNEYGDLRFLDPRPVEREPLSFRDLTLVPLHHRHGDTAVVS
                     FRTGPFAFLADLSAIDDAELEKLAGISTLVISCTVRHDHYKHLKLDEVLALAARIGAP
                     RTVLTHMNHRFNHADLLATLPQGVEPGYDGLALEF"
     gene            6271..>9728
                     /locus_tag="PKH10_02230"
     CDS             6271..>9728
                     /locus_tag="PKH10_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26974.1"
                     /translation="MRKVLLALSALCFLVSCQVEIRQFEVVNQSGPYNCSDTGQCEMK
                     VLVLKDGRPLTGYTMTDAANYLFEYEVVGDNGYLYHYRSYLDKAILKGDVIVYPSGYL
                     VFRHTFPSTANYEVFIHFYDYRFNGLWDRIEIPIVINPCYNQRGVCANGTCVPVGRFD
                     YRCDCADGYVHPADDDRQCLLDRCRGERCNGNGVCRLDGQGDPHCDCFLGYGHGDLNA
                     RMAPTVTDGTPDPLSCIPVACDEEGDTTGTVRYMVRPLTVSLQKTAEEQPDEAPREIL
                     ALDEIRLPEVTQGCEIVNDDEVAGTALGKNSFTEFMKVDRAELARFFYGDISSGTVPF
                     AVKHPHILTRSLLGDTGALSMLDRFKDNPVFAERLQWVTERHLSSCEDYIYKKYLMYN
                     LWLDVFAESSDDPYLIVRFSATPGAACETGSVTEGFGDDRCMGLLSPEDFASSYGGRI
                     LYFGARHVAGDPYPVAGFDASKFSLSTPRYVPNNPFVLTAHKKNGYIRQPNDPYWGHA
                     HDFRWHKQMFDLANSGYWKDTYGADIANRHFDRYRLGRELLLELLVNTILFRDLNSTE
                     FDLSDGKEAVKSDLEKVALLGGWLQDVLKKREKNGVWPALIQYALDNGAPVEGSMKNC
                     YSERYYEKNVECKTMKDPLWSDIPHAYPENVIIESWSILTRNEKDRVLSLLDKKVDTL
                     TSVARYGTQLADRLINGAEDTYYGEIENDLDLLRRALELLNDVKSADVTGLLDAKIEE
                     LLALAAGADCLKSNAPKEGVSKLQQICGWLPEMFVESVRSDIEHAEPLSEAEAQAFSD
                     GAGDLPPIVNLPAATPPDIVKAKVNAFSGKSVMDIMQIDHRECRSKIPEPTVDRSSFE
                     KLGDFRDFTCKGSVGQIVDLTYNCFFNLDYSGCCTERLGNYDITIKNVIKVDENTYDF
                     RFSDLITQNPRGFDACEEGISEIDGKELFLRRYGILGLHTLPGKLPDTNLCWHTCGQP
                     GEDCNALKYHHCTLLEDGEKIVYAPPNTEGTNSLSFFSCQGEAARFYEFTEDITRYSC
                     EHFSDDQGGDETSYTPMWGIESYERDFMRRNLLDQIKPSPTFKRWFSLYALDKNSLPS
                     YHNYFEVSDPSADGPFEEKDFSRIVFYEEEYAAACKIEGAWKITLHDSGRRSYFDETD
                     LLNGER"
BASE COUNT         2056 a         3209 c         2685 g         1778 t
ORIGIN      
        1 acctgtcgcc cgagcagtgc cagaccaaca cgatcgatca ccggagcgac ctgtacgcgc
       61 tcggcctcat attccacgag gcgctggccg gagaaccgcc gttcgccgaa cccccgggca
      121 cgctcgcgcc ggccgcgctc tccccggatg ccatcgtctt ccccgaggcg ttcgacctcg
      181 acatggcccg catcatcaag ggcctgctcc agcccgaccc cgcggaacgg acgcccgacg
      241 cgctcaccct gctcaacgaa ctcgagtcgc tcgaaagcaa actgatcatg gcccgcgcgg
      301 ccaaacgccc cgcccgcgcc ctgccgctcc gcatccacga acccgaattc atcgcgcgac
      361 gcgccgagat ggcgcgcctc gtgaccgcga tagataattt cctctcctcc ggcctcgaca
      421 tgacggtcat cgtcgaaggc gaacgcggcg ccggcaagac ccggttcctc gaggaggtcc
      481 gtgcccgcat ggcgttccgc gacctttcga ccgtgctcgt gtcggccgcc gacacgcaaa
      541 gtctcgcgag cgatctcctc aaggggctct gggacaccgc cgaccgcgaa ctgcgttatc
      601 agatagcggt gaaatggggc gcgctcatcc ggacctattt ccccgaatgc gccgtcgccc
      661 ccgagttccg tcgcatcacc gtccccgccg ccgaggaaca ttcgatggag gagaaccttc
      721 accgcgtcgt cgcctgcatc ggtgacatca tcgagacgat cacccgcacg cggccactcc
      781 tcctgttcat cgacaatttc ggcgacatcg acaagcggtc gctcgccgtg atgcaggaac
      841 tgtacgcgca cgccgagaac taccggcgcg tgttcaccac gctcgccgtc accaccgaga
      901 acgccgtctc ccccatcgag ttcccggcca acgcccgcgt cacgataccg agtttctccc
      961 cgggcgaact gcgcgatttc atcgagtcgt gtctccagtc gccgcgcaag catatcgacg
     1021 aatcgctctt cctctggctg taccgcatga gcggcggcat cgtcaagacc gccttctcgc
     1081 tcctgcacat gctccgcgag gagggtttcc tgctgctttc cggcgaacgg atactgttcc
     1141 gcaacgatgt gctcgaaaag gaaccgatag acttcctgtt ccgcctcaag ttgaattcgc
     1201 tctcgtccga tcagcgggcg ctcctcaacc tcgccgccat ctaccggaaa ttcaccaccc
     1261 gcgaggcgct gtccgccgtg ctcctcgaac ggatgtccga cgaagcgttc cgcgacgcgc
     1321 tcgatttcct gaaaaggaac tatctgctcg aagagcacaa gaacggcaag atacggctcg
     1381 tgaacgcccg cctgcaggcg ctcgtctacc agagcatgcc ggccgacgag cggcggcgcc
     1441 tgcacctcag ccacgcggac tacctgctca cgagcaaccc gctcgaggag ataaacacct
     1501 gcgccttcgc cgcctatcac tacctgcggg gcggccggcc cgagacggcg ctcaaatact
     1561 acctgcgttc ggcgagccgc gcgttcttcc atctcaacac cgaactgacc ggcagtttca
     1621 tcgacgaggc gctctccatc gtccgcgaga acccctacat cctctcgctc aagaaaaaaa
     1681 cggcggtctc gctcttcgcg ggccgcatct tccaccgact gggcatctac ggccgggcga
     1741 tccccctgct cgaagaggcg tacggcacct ggaaggacga actcatcctc gacctgctgg
     1801 tccgcgcgct cgtcggcgac ggcgacgccc cgaaggcgct ccagcacacg gtcaaattca
     1861 aggccgacac ccccgaacgc gccgcgttcg tcgcctatct gcgcgcctac atcgcgggtc
     1921 gcgccgaaga gaactacgcc aaggcggcgt cccacataca gaaggcgaag agactcatgg
     1981 ccgccggaca cgacgcactg ttcaccccgc aacgccgcta tctcctgcgc gaactcgagt
     2041 tcgaccacgc catgtatgaa cgcggaagcg attacgagac gctccttacg gtgcgcaacg
     2101 acctcatcga gagcgcgaag aaactgccgc gcaaggccta tctcatcgac gcgctcaccg
     2161 cgtcgttccg cttcctccag cggttcaaca aactcgagaa gggctacctg ctcctccagc
     2221 agacgctcaa actcgccatc gaatcgttcg acaacttccg catcgcccgc gcctatctca
     2281 atctcgcctc gtgcgcccaa ctgctcgaac ggtggcacga ggtgccgttc ttcctcgacc
     2341 gcgcgatgga tttcgcgcgc aaaggctgcg gcaccaccat gctcaaatac gccttcttcg
     2401 cccgcggcga gttcgccgtg ctcacgggtg acttctcgct cgccgagaac tacctgttca
     2461 acgccgagga actgtcgtac cgcgaacggc gcgacagcga cctcatcggc atctacgcgg
     2521 cgcaggtgct cctctacctg ctcaagggcg aatcgggttt cgcgcgcgtg gcgggcggca
     2581 aactgcgccg gttcctcgac aaaaagaagc tcgacgacca gcggcgatgc cgcgcgctct
     2641 gcacgctcct gttcctcgag gcgtcgttcg agggcgaccg cgaatacatc ctcgaactgt
     2701 cggatcagat agaggcgctg ttcaaacaac acccggaact gcgaccgctc taccggcttc
     2761 tctatcttac ggcgcgggcg ctttacgccg cccagaaagg gtacgaccgc gaccttaccc
     2821 gcatcgtcga aacgatagag gacgaaaagg tctccgccgt cgcgccgctg ttccgcctgc
     2881 tgtatcgcca ccggatagcg cacatcctca gcgagaaaaa ggccctgccc gagatcctgc
     2941 gacgcctcat agacgagggc cggcaggaat cggcgctcct gcaggcgcgc cacttcatca
     3001 cgctgttcgg agaactttcc tatttcatcg actacgggca gaccgaccaa ctgatggccg
     3061 aggtacgcgc cgccatcgac gaggccgcgt ccggcgaccg gagcgaaagg acgctcggcc
     3121 gcctgaaact ctattttgac gagacgcgtc gccagaccga cttcctgcgg acccgtaacc
     3181 gcaactattc ctacatcatc gacatcgtca aggcgatatc ggggaagacc gaggtcggca
     3241 agatcatgga catcgtcgtc aagaagatac tcgacatcct gtcggcggac ctcgtcgccg
     3301 tcgtgttcca gtacgacgac ggacggcaga tggactatct cataaaggac gccgcccagc
     3361 agacttacaa attcaacgac atccggttca agggcgacat cgtccagcgt atgttcaaga
     3421 cggccaagat agagttcttc accagcatgc ctctgagttt ctccgacacc gactcctccg
     3481 cctcgtcggg cccggacgcc gacaactgcg tcgtggcggc gataccggtg ctcctgcagg
     3541 gcgagatacg cgcctatctg tacctcgaac gcaacctctc gaaaggcgcg ttcctcgagg
     3601 aggagataca gttcctcgag atactcgccg acaacatcgg cgtcatattc gacaatgtga
     3661 aactggtcga gatcgcgacg accgacgcgc tgacccgcgt ggcgacccgc cgccatttcc
     3721 tctcgatact caagaaagaa tgcgaaaagg cgcgccggta cggcttcacg ctctcgctca
     3781 tcatgataga catcgacctg ttcaagaaca tcaacgacac ctacggccac ctgatgggcg
     3841 acaccgtcct gcgacagatc ggtcgtctgc tcaagaacaa catccgcaac tccgattatg
     3901 cgggtcgcat cggcggcgag gagttcgcgc tcctcctgtc gggcaccaac gccacgggcg
     3961 ccttcaacac cgccgagaag atacgcgaga aatgtcagcg ttccagtttc tccgggctcc
     4021 agctcacgct ctcgaccggc atcgcgtcct accacgagca cgaggtgaag aacgaggagg
     4081 atctcctgca gaaggccgac acggcgctgt acgccgccaa acgccggggt cgcaacatga
     4141 cggtacgcta cgacgacagg gagggatgat gccggtaacg atatttgtcg aggagaacag
     4201 catagtcgcc gggaacaccg acaaggtgcg ccgcacgctc gaaccgatcg tggaaaattc
     4261 cgatatggtg cgccgctacg aggcggggct cgcgctctct ttcggaccgg cgcccggcaa
     4321 acgaccgctc gggttctttg acttggagaa gcggctctcc cagccatcct acaagcactg
     4381 gttccgccgc atggaccgcg aggtgccggc gctcccctac ttcctcgcgg ccgacgaccc
     4441 ggggaaatcg ctactcatct atctcatggg ggtgctcgac tacaaactca tcggtgaacg
     4501 ggtcaccttc gacgcggccg cgatggatgt gttcttccac cacaagaaga ccgaaataaa
     4561 gaatctctgc ctctcgaaca acatcagccc gctggcgagc atccagcggc tcgccgacct
     4621 gctcgccccc gcggcccgtt ccgacggccc cgtcgccaca ccgccgcccg ccgccgcgcc
     4681 gccggcgaaa gaggagcggc ccgacaccga ccgcccggcc gaggcgcaac tgctcaaaca
     4741 actgctcgac cggttcggct ccatcgcggt gctgaaagag aaccgcatga cgaaactgtt
     4801 cgtggtcttc gacgagatac cgccgcagat acggctcgtc cgcaacgaac tggtgctcga
     4861 ccggtcgctc aaccgctggt tcttccgcac cgtgttcctc atcgacgggc gcgagtcggc
     4921 gcgggaatcg ctcctgctct accgtccgaa cgaggtgacc gaatcgatac aattcttcaa
     4981 cggcgcgctc atcatgtgcg tctaccgcga cgaaacgagc gaataccaga aactgttcga
     5041 gaccgacgag ccggtgagga ccgatatcgt cgaacaggct ggacacgccg tcgccgaagt
     5101 gtccggcggg caggccgcgc cggatggccc cgccgacgag ggaacgaccg ggcaggcatc
     5161 cccgccgccc gccgccgcgc cggaacctgc cccgcaggcc gcgccggccg acccgaaaga
     5221 tgcccggatc gccgaactgg aggccgaggt ggcgaaactc aagaacatcg tcgaggccta
     5281 cgaggagacc ctgctcggca agaacaagaa aagcatcttc aagaagttct tcggatgatg
     5341 cgcgtcaccc tgcttggcac cggctcgtcc accgggaccc cgcaactcct gtgcgactgc
     5401 gagaactgcc gctcgaccga cccgcgcgac cggcgtacgc gtttcgcgat acttcttgaa
     5461 cagggcgaca ccgtcctgct cgtcgatgcc ccgttcgaga tacgcctgca actgctcgcc
     5521 gcgaaggtga agcggctcga cgcgctctgg ctcacccatg cccacagcga ccacctcgcc
     5581 ggcgtggacg acctgcgcat cttcgcattc cgcaacggcg cgccgatacc ctgcttcgcg
     5641 ctttccgaga ccatcgcgac gactcgacac cgcttcacct acctgttcga tgacaacgaa
     5701 tatggcgatc tccgcttcct cgacccgcga ccggtggagc gcgaaccgct gtcgttccgc
     5761 gacctcacgc tcgtcccgct gcaccaccgt cacggcgaca cggcggtcgt cagtttccgc
     5821 accgggccgt tcgccttcct cgccgacctg tcggccatcg acgatgccga attggagaaa
     5881 ctcgcgggca tctcgacgct cgtcatctcc tgcaccgtcc ggcatgacca ctacaagcac
     5941 ctgaaactcg acgaggtgct cgcgctcgcc gcccgcatcg gcgcaccccg cacggtgctc
     6001 acccacatga accaccggtt taatcacgcc gacctgctcg ccaccctgcc gcagggcgta
     6061 gaaccgggct atgacgggct cgcgctggag ttctgagacc cgccgtcccg ctccccgaaa
     6121 aaatcactga atgatatcgg gcagaaatca ttttctttca aaatcctcgc gaaactctct
     6181 tgactttcga aatggtcgcg aaatagaacc ataccgtcac acgaccgttc tttttcaaaa
     6241 aaactttgaa cctgtcgcat gggggtgcgt atgagaaaag tcctgctggc cttgtcggcg
     6301 ctgtgtttcc ttgtttcctg tcaggtagag atccgccagt tcgaagtggt caaccagtcc
     6361 ggtccctaca actgttctga caccggacaa tgcgagatga aggtccttgt gctcaaggat
     6421 ggtcggccac tcaccggtta cacgatgacc gacgcggcga attatctctt cgaatacgag
     6481 gtggtcggcg acaatggcta tctgtaccat tatcgctcct atctcgacaa ggcgatactg
     6541 aaaggcgatg tcatcgtcta tccctccggc tatctcgtct tccgccacac ctttccttcc
     6601 accgcgaact atgaggtgtt catccatttc tacgactatc gcttcaacgg actttgggac
     6661 cgcatcgaga tacccatcgt catcaatccc tgttataacc agcggggcgt ctgcgcgaac
     6721 ggcacctgcg taccggttgg ccggttcgac tatcgctgtg attgcgccga cgggtatgtg
     6781 catccggcgg atgacgaccg gcagtgcctg ctcgaccggt gtcgcggcga acgatgcaac
     6841 ggcaacggcg tctgccgcct cgacggacag ggtgatccgc actgtgactg tttcctcggc
     6901 tacggacacg gcgacctgaa cgcccggatg gcacccaccg taacggatgg aacacccgac
     6961 ccgctcagtt gcatacccgt cgcgtgcgac gaagagggag acacgaccgg cacggtccgt
     7021 tacatggtcc gccctctcac cgtgtcgttg cagaagacgg cagaggaaca accggatgag
     7081 gcgcccagag agatactggc gctcgacgag atacgcctcc ccgaagtgac gcagggttgc
     7141 gagatcgtga atgatgacga agtagccggc accgcgctcg ggaagaacag tttcaccgaa
     7201 ttcatgaagg tggaccgggc cgaactggcg cgattcttct acggcgatat ttcctccggg
     7261 accgtcccgt tcgccgtcaa acacccgcat atcctcaccc gttcgcttct gggggatacc
     7321 ggggcgctct ccatgctcga ccggttcaaa gacaaccccg tcttcgccga acggctccag
     7381 tgggtgaccg aacgccacct gtcgtcctgc gaagattaca tctacaagaa atacctcatg
     7441 tataacctct ggctcgatgt gttcgccgaa tcatcggacg acccgtatct catcgtcaga
     7501 ttctcggcga caccgggggc ggcctgcgag acggggtcgg tcaccgaagg tttcggcgac
     7561 gatcgttgca tgggcctgct ctcgccggag gatttcgcat cctcgtacgg cggtcgcatc
     7621 ctctacttcg gcgcgcgcca tgtcgccggg gacccctatc cggtcgcggg ctttgacgcg
     7681 tcgaaattct cgctgagcac acctcgctat gtcccgaata accccttcgt cctgacggcg
     7741 cacaaaaaga acggctatat caggcaaccc aacgatccct actggggcca cgcccatgac
     7801 ttccgctggc acaaacagat gttcgatctg gcgaatagcg gctactggaa agacacctac
     7861 ggcgccgaca tagcgaaccg ccatttcgac cggtaccgcc tcggacggga actgttgctg
     7921 gaactgctcg tcaacacgat cctgttccgc gacctcaact ccaccgaatt cgatctctcc
     7981 gacggaaaag aggcggtcaa gagcgatctg gaaaaggtcg cactgctcgg cgggtggctg
     8041 caggatgtgc tgaagaagcg cgagaagaac ggggtgtggc ccgcgctcat acagtatgcg
     8101 cttgacaacg gcgcgccggt ggaaggaagc atgaagaact gctacagcga acggtactac
     8161 gaaaagaatg tggaatgcaa aacgatgaaa gatcccctgt ggtctgacat ccctcatgcc
     8221 taccccgaga atgtcatcat cgaatcgtgg agcatcctca cgcggaacga gaaggatcgg
     8281 gtgctgtcgc ttctcgacaa aaaggtggac acgctcacca gcgtggcgcg atacggcacc
     8341 caactggccg accgcctgat aaacggcgcg gaggacacct attatggaga gatcgagaac
     8401 gatctcgatc ttcttcggcg ggcgctggaa ctgctcaacg atgtgaagag cgccgatgtc
     8461 accggactgc tggacgcgaa gatcgaagaa ctgctcgcgc tcgccgccgg ggcggactgt
     8521 ctgaagtcga acgcacccaa ggagggggtc agcaaactcc agcagatatg cggctggctt
     8581 cccgagatgt tcgtggagag cgtccgtagc gacatcgaac atgccgaacc gctcagtgag
     8641 gcggaggcgc aggcgttcag cgacggcgcc ggcgacctgc cgccgatagt gaacctgccc
     8701 gcggcgaccc cgccggacat cgtcaaagcg aaggtcaacg ccttcagcgg gaaaagcgtg
     8761 atggacatca tgcagatcga tcaccgtgaa tgccggtcga agataccgga gccgaccgtg
     8821 gaccggtcct cgttcgagaa actcggtgac tttcgcgatt tcacctgcaa gggatcggtg
     8881 ggacagatag tggacctgac ttacaactgc ttttttaatc tggactacag cggctgctgc
     8941 accgaaaggt tgggcaatta tgatataaca atcaagaatg tcattaaggt cgacgaaaac
     9001 acctacgatt tcaggttttc agatctcatc actcaaaatc cccgaggttt cgatgcctgc
     9061 gaggagggta tttcggaaat agacggcaag gaactttttt tgagaagata cggtatcctc
     9121 ggtcttcata ccttgccggg aaaattgcct gacaccaatc tttgctggca cacctgtggt
     9181 cagccgggcg aagattgcaa tgcccttaaa tatcatcatt gtacactgct agaagatgga
     9241 gaaaaaattg tttatgcgcc cccaaacacg gaggggacaa actctttgtc ctttttttcc
     9301 tgccagggag aagcggcgcg cttctatgaa ttcaccgaag acatcacgcg ttatagttgt
     9361 gagcactttt cagatgatca gggaggagat gagacctctt atactcccat gtggggcatt
     9421 gaatcgtatg aaagagactt catgagacgg aacttgcttg atcagataaa gccctcccct
     9481 acttttaaaa gatggttctc tctctatgcc cttgataaaa attctttgcc ttcttaccac
     9541 aactattttg aggtctcgga tccttcagcg gatggccctt ttgaagaaaa agatttttcc
     9601 cgcattgttt tctatgaaga ggaatatgcc gctgcctgca agatagaagg ggcatggaag
     9661 attactctcc atgactccgg ccgtcggtcc tatttcgacg agaccgacct gctgaacggc
     9721 gagcggat
//
LOCUS       DAOQUE010000041         9725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_7833_length_9725_cov_4.435574, whole genome shotgun sequence.
ACCESSION   DAOQUE010000041 DAOQUE010000000
VERSION     DAOQUE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7833_length_9725_cov_4.435574"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PKH10_02235"
     CDS             <1..843
                     /locus_tag="PKH10_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNT26975.1"
                     /translation="LKKVALQRRGLVLVTGTTGSGKSTTLAAMIEHINANRTCHIVTI
                     EDPIEFLIRDKKSIVNQREVGLDTRSFSRALRSALRQDPDVIMVGEMRDLETIEIALT
                     AAETGHLVLSTLHTLDAAETVNRVVGAFPTHQQSQIRQQLASVLTAAVSQRLLKNKDG
                     RGRVPAVEVLLNTSLVKEIVLDLSRLKELPGIIERGHTNYGMQSFDQSIYQLFRDGHI
                     TQKEALDNATSPDDLVLRMKGVAISGDGDWSAFDDKGNQFGQKVEKNASVDSKTIDDE
                     FELG"
     gene            821..1246
                     /locus_tag="PKH10_02240"
     CDS             821..1246
                     /locus_tag="PKH10_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNT26976.1"
                     /translation="MTSSNSADTAACLRRALSLLARRDHFSTELVDKLVEKEFSREDA
                     QTALNKLRELRYLDDRRILEAYAAEMKRRKKGFLFFCKKLIEKKARSLFSDRELREVY
                     TVAEERAIAKALAAKMRWDRNEARRRLATRGFSADAANG"
     gene            1322..2602
                     /gene="glyA"
                     /locus_tag="PKH10_02245"
     CDS             1322..2602
                     /gene="glyA"
                     /locus_tag="PKH10_02245"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNT26977.1"
                     /translation="MKHIPSVDPEIAKIIEEEAERQEFGLELIASENFTSKAVMEAQG
                     SILTNKYAEGYPAKRYYGGCEVVDKAEQLAKKRACELFGAEYANVQPHSGSQANMGVY
                     FAALKPGDTILAMNLDHGGHLTHGSPVNFSGKLYKIVPYGVSKETGRIDYDELERLAV
                     EHRPQMILAGASAYPRTLDFPRFRAIADKVKAYFAVDMAHIAGLVATGHHPSPVPYAD
                     FVTTTTHKTLRGPRAGLILAKKEFEKILNSAVFPGMQGGPLMHIIAAKAVCLKEAMSP
                     EFKTYQGQVLKNAAAMADEFTKLGYNLVSGGTDNHLMLVDLRNKGKTGKIVQNVLDLA
                     HITVNRNMVPFDTESPFVTSGIRVGAPFITTRGLKEDDARLVIRLMDKVIMAIKTNEI
                     PKGGVDETVVPKAVLDEVTAEVKKICNRFPLIRK"
     gene            2605..3078
                     /locus_tag="PKH10_02250"
     CDS             2605..3078
                     /locus_tag="PKH10_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNT26978.1"
                     /translation="MTPSTRPPRPSWDEYFMEFARLAARRSSCLRRSVGAVLVRDKRV
                     LATGYNGVPTGITHCSETGCLREKLKVPSGERHELCRGLHAEQNAIIQAAHHGISIEG
                     STLYCTTHPCIICTKMLINAGIVGVVYAEGYADELSAEMLKESGITIKRYEDGAL"
     gene            3075..3536
                     /gene="nrdR"
                     /locus_tag="PKH10_02255"
     CDS             3075..3536
                     /gene="nrdR"
                     /locus_tag="PKH10_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNT26979.1"
                     /translation="MKCPFCGSDEDKVIDSRAIKDLAEIRRRRECITCGRRFTTYERI
                     EEVMPQVVKKNDVREPFDRSKIRRSIEIACRKRSISQEQIEQMVKEIERTVQELDRRE
                     ISSQVIGEQVMKHLRKMDKVAYVRFASVYREFKDVDDFIDQVQELGKDARK"
     gene            3562..4665
                     /gene="mnmA"
                     /locus_tag="PKH10_02260"
     CDS             3562..4665
                     /gene="mnmA"
                     /locus_tag="PKH10_02260"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNT26980.1"
                     /translation="MTSSRAVIAVGMSGGVDSSVAAALLVAAGHRVVGVTMAIWDGST
                     AVAEGSKHACFCAGEEEEIATAARVAERIGIPFHVIDLRQEYRAEVLDHFRREYLAGR
                     TPNPCVRCNHLMKFGFLLERARAQGLSFDRFATGHYARVVPGADRLFLKKAYDTAKDQ
                     SYFLQFLTRDQLGSVLFPLGEKTKGEVRETARRLGLPVAERPDSQDFIAGDYGILFSP
                     EEAPPGDIVNEKGALLGRHRGIVHYTVGQRRGLGVDGGKPLYVTAIDARNNRIVVTDK
                     DHLFGRGLVAGQVNWLGMDRPDRPFRAHARIRQKHSEAPATVIPRDDGTITVRFDEPQ
                     LSITPGQAVALYADDTVLAGATISQRTDEGADT"
     gene            4662..5303
                     /locus_tag="PKH10_02265"
     CDS             4662..5303
                     /locus_tag="PKH10_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26981.1"
                     /translation="MKRICWGFCLILLAAMPLAADGPEQPQGPEKDRPAAEEKGDEPV
                     ADGGRVYLKVGGGLLFDERRVVVEPAFGFGYRYTQGAFAVDLSVFNETIGPTVDYAGY
                     KEEQEYERNALRITMSRWIGLSFLWVAEPRAWNSFFAGGGLAIETLDLELASESSSQT
                     GLGVTLVAGWEFLRDRTVILFAQAEVDLPCYTARFYGDRFWVPSIVFVIGAGL"
     gene            5376..5451
                     /locus_tag="PKH10_02270"
     tRNA            5376..5451
                     /locus_tag="PKH10_02270"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5409..5411,aa:Phe,seq:gaa)
     gene            5550..8651
                     /locus_tag="PKH10_02275"
     CDS             5550..8651
                     /locus_tag="PKH10_02275"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT26982.1"
                     /translation="MKRQIVALLMFLTATTAAAGDIPWSNTDTGAIAERIPELSGFSV
                     DGVAVDLPRRLLTVRLTLPASLTLDERILDEMNRLLVSMLAQKKLPFRTLVILARRTG
                     QTEWRDIRSLLPTLPPVPRKEHEPVASEPNPYLGFPRSGTTGTAGALAGKTVYLSAGH
                     GWYYNGSAWTTQRGNTVGIVEDLSNTEIISYYLAPMLRNAGALVFTARESDRNAVSVT
                     VDDADATNLPANGAYEESDGWTESTGTGWGRLTTPFATGDAPFSAGHYRVTEPATGRW
                     ARWTPNLPTGGRYQIFVSFRPMSDRAPTARYEVRHAGGMTTVVVDQRHHGNTWFPLGE
                     FTFFGGYSVDKGSVTLLADTTAGGSYVIADAVRFGGGTGIIDRDGGVSGRPRWEEAAR
                     YHAQLLGAPATVYDTSDTDNSDDVTTRSRWAAWENESGWDDSVFISWHSNAYNGNARG
                     TSTYVYGPNDPGTGWEPAAQEGSEELATLLHNGVVAALRDGWDAAWPVSGSGLYSAWF
                     GELNPSYNNEMPACLIELAFHDNTTDSNALRDPRFRLIAARAATQAVIQYFADRDTLE
                     AVYPPDTPRMVRTTAIGPHEAIISWEAPPDSPRTLYGDAPTGYIVQRSADGFAFDDGV
                     EVEGATSFTAQDLYPYSVDYFRVIAYNDGGRSFPSPVVAVRPKSDGAQVLIVDGFERL
                     DRGLLVSQDLSSYGLGAVMRMFLERMNTFDYVRAHALALSDLSYSFDSVQKSALETID
                     LTVYDAVLWFTGEQSTADVTVTPAEQTHLCDYLDGGGILFISGSEYGWDLYHMGDATD
                     QAFFTDCLGLSYLGDDAGTYQAATVTGGEDVFNGSLSFDDGSFIYNADYPDLIEAAGD
                     GITFLGYGDGTGGGAGVVTGGDRKVAALGFPFETILDADTRRTLLGGILAWYDLTALE
                     EPVDEDVIESDNEISDDHAAPVDDGDADPDYGGGCAEGDYSFSDEDATVVDETTVDED
                     TVFSDDQSDQSDQPDQADQPDQTDQPDLYGLPDETTVDELIIGEDSDTAKGSSSGCGC
                     SVI"
     gene            complement(8653..>9725)
                     /locus_tag="PKH10_02280"
     CDS             complement(8653..>9725)
                     /locus_tag="PKH10_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HNT26983.1"
                     /translation="DGRPFAQYPRNVVRATLDHLKKQKIADTAKMLVELEFYVFDEVS
                     YATDFGHSYYKVQSPEGIGEEYYDQPRFGIHRGYHRQPPDDRHFALRNTVVATMEAIG
                     IPVKYHHHEVGAAQLEIELNFLPLLEAADGVTLAKWIIKSAAREHNLFVTFMPKPIYK
                     VAGSGMHVHQFLEKGGESLFPGKALYNLSPLAHSYLCGILEHSLTGSLLALTNPSTNS
                     YKRLVPGFEAPVNATFAKASRAAAIRIPGYLKGKDVRIEFRTGDATANTYYMLAAMVL
                     AGIDGIRRKADPVKLGFSDMKNEKSFPLDLNLVLDGLQKDRRYLLPAFPEQLLDLWIK
                     IKRDEATHVYNAPTPQEYELYF"
BASE COUNT         1886 a         3128 c         3032 g         1679 t
ORIGIN      
        1 ctcaagaagg tcgctctcca gcggcgcgga ctcgtgctcg tgaccggcac caccgggtcg
       61 ggcaagtcga cgacgctcgc cgcgatgatc gagcacatca acgcgaaccg cacctgccac
      121 atcgttacca ttgaggatcc catcgagttc ctgatacgcg acaagaaatc gatcgtgaac
      181 cagcgcgagg tcggactcga cacccgcagt ttctcgcggg cgctccggtc ggcgctccga
      241 caggatcccg atgtcatcat ggtcggcgag atgcgcgatc tcgagacgat agagatagcc
      301 ctcaccgccg ccgagaccgg gcatctggtc ctctcgacgc tccacacgct cgatgccgcc
      361 gaaacggtca accgcgtcgt cggcgccttc ccgacccacc aacagtcgca gatacgtcag
      421 caactcgcgt cggtcctcac cgccgccgtc tcacaacgcc tcctcaagaa caaggacggt
      481 cgcggccgcg tcccggcggt ggaggtcctc ctgaacacct cgctcgtcaa ggagatcgtg
      541 ctcgacctgt cgcggctcaa ggaactgccc ggcatcatcg agcgcggcca caccaattac
      601 ggcatgcagt cgttcgacca atcgatatac caactgttcc gcgacggtca cataacccag
      661 aaggaggcgc tcgacaacgc cacgagcccc gacgacctcg tcctgcgcat gaagggggtc
      721 gcgatatcgg gcgacggcga ctggagcgcg ttcgacgaca aggggaacca gttcggccag
      781 aaggtcgaga agaacgcatc ggtcgattcg aagacgatag atgacgagtt cgaactcggc
      841 tgacactgcc gcctgcctcc ggcgggcgct gtcgcttctg gcgcggcgcg accatttctc
      901 caccgaactg gtggataaac tcgtcgagaa agagttctct cgcgaagatg cgcagacggc
      961 gctgaataaa ctccgggaac tgcgctatct cgacgaccgg cgcatccttg aggcctatgc
     1021 cgccgagatg aaacggcgta aaaaaggatt cctcttcttc tgcaagaaac tcatcgagaa
     1081 aaaggcgcgg tcactttttt ccgaccgcga actgcgcgag gtctacaccg tcgccgagga
     1141 acgggcgata gccaaagcgc tcgccgcaaa gatgcgctgg gaccggaacg aagcgcggcg
     1201 gcgcctcgcc acgcgcggct tctcggccga cgcggcaaat ggttgacaaa cctttcccgc
     1261 cccgttagaa tcgcctccgt tcggaatctg aactttttgt ggaggaatat ccatgcttga
     1321 cctgaaacac atcccatcgg ttgaccccga gatcgcgaag atcatcgagg aggaggcgga
     1381 gcggcaggag ttcggccttg aactcatcgc cagcgagaac ttcacctcga aggcggtgat
     1441 ggaggcgcag ggatccatcc tgaccaataa atacgccgaa ggctaccccg ccaagcgcta
     1501 ctacggcggc tgcgaagtgg tcgacaaggc ggaacaactg gccaagaaac gggcatgcga
     1561 actgttcggc gccgaatacg ccaatgttca gccccacagc ggatcgcagg ccaacatggg
     1621 cgtctatttc gcggccttga agccgggcga caccatcctc gcgatgaacc tcgatcacgg
     1681 cggccacctg acccacggga gcccggtcaa cttctcgggc aaactctaca agatagttcc
     1741 ctacggcgtc tccaaggaga ccggccgcat cgattacgac gaactggaac ggctcgcggt
     1801 cgaacacaga ccgcagatga tactcgccgg cgcctcggcc tacccacgca ccctcgattt
     1861 cccgcggttc cgcgccatcg ccgacaaggt gaaggcctac ttcgcggtcg acatggccca
     1921 catcgcgggg ctcgtcgcca ccggccacca cccgagcccg gtcccctacg ccgacttcgt
     1981 gacgacgacc acccacaaga cgctccgcgg cccgcgcgcc gggctcatac tcgccaagaa
     2041 ggagttcgag aagatactca actcggccgt cttccccggc atgcagggcg gcccgctcat
     2101 gcacatcatc gccgccaagg cggtctgtct caaggaggcg atgagtcccg agttcaagac
     2161 ctatcagggg caggtgctga agaacgccgc cgcgatggcc gacgagttca cgaaactcgg
     2221 gtataacctc gtgtcgggcg gcaccgacaa ccatctgatg ctcgtcgacc tgcgcaacaa
     2281 agggaagacc ggcaagatcg ttcagaatgt actcgacctc gcccacatca ccgtcaaccg
     2341 caacatggtc ccgttcgaca ccgagagccc gttcgtcacg agcggcatcc gcgtcggtgc
     2401 ccccttcatc acgacccgcg gcctcaagga ggacgacgcg cgtctagtga tccggctgat
     2461 ggacaaggtc atcatggcga tcaagaccaa cgagataccg aagggcggcg tcgacgagac
     2521 ggtcgtcccg aaggcggtgc tcgacgaggt gaccgccgag gtgaagaaga tctgcaacag
     2581 attcccgctg atacggaagt gagcatgacc ccctccaccc ggcctccccg cccctcgtgg
     2641 gacgagtatt tcatggagtt cgcgcggctc gccgcgcggc gttcctcctg cctgcggcgc
     2701 agtgtcgggg cggtgctcgt ccgcgacaag cgggtgctcg cgaccgggta caacggcgtg
     2761 ccgaccggca tcacccactg tagcgagacc ggctgtctgc gcgagaaact caaggtgccg
     2821 tcgggtgaga ggcatgaact ctgccgcgga ctccacgccg aacagaacgc catcatacag
     2881 gcggcccacc atggcatatc catcgagggt tcgacccttt actgcacgac ccacccctgc
     2941 atcatctgca ccaagatgct catcaatgcc ggcattgtcg gcgtcgtcta cgccgaagga
     3001 tacgccgacg agctgtcggc cgagatgctc aaggaatcgg ggataaccat taaaagatac
     3061 gaggacgggg cgttatgaag tgtccgttct gcggcagcga cgaggacaag gtcatcgaca
     3121 gccgcgccat caaggacctc gccgagatac gccgacgacg cgaatgcatc acctgcggcc
     3181 gccgcttcac cacctacgag cggatagagg aggtgatgcc gcaggtcgtc aagaagaacg
     3241 atgtgcgcga acccttcgac cgctcgaaga tacgacggag catcgagatc gcctgccgca
     3301 agcggagcat atcgcaggag cagatcgaac agatggtcaa ggagatagaa cggaccgtgc
     3361 aggaactcga ccggcgcgag atatcgtcgc aggtcatcgg tgaacaggtc atgaagcatc
     3421 tccgcaagat ggacaaggtc gcctatgtcc ggttcgcgag cgtctaccgc gagttcaagg
     3481 atgtcgacga cttcatcgat caggtacagg aactcggcaa ggacgcccgt aagtgatctc
     3541 accggagcgg gggctaacgg catgacctct tcccgcgctg tcatcgcggt cggcatgagt
     3601 ggcggcgtcg actcatcggt cgccgcggcc ctgctcgtcg cggcgggaca tcgtgtcgtc
     3661 ggcgtcacga tggcgatatg ggacggttcg accgcggtcg ccgaggggtc gaagcacgcc
     3721 tgtttctgcg cgggcgagga ggaggagata gcgaccgccg cccgcgtcgc ggagcggatc
     3781 ggcatcccgt tccatgtgat agacctccgg caagaatacc gcgccgaggt gctcgaccat
     3841 ttccggcgcg aatatctcgc cgggcggacc ccgaacccct gcgtccgctg taaccacctc
     3901 atgaaattcg gctttctcct cgaacgggcg cgcgcgcagg ggctgtcgtt cgaccgtttc
     3961 gcgacgggtc attacgcccg tgtcgttccc ggcgccgatc gcctgttcct gaaaaaagcg
     4021 tacgatactg cgaaggatca gagttatttt ctgcagttcc tgacccgtga ccagttgggg
     4081 agcgtgctgt tcccgctggg cgaaaaaaca aagggcgagg tacgcgaaac ggcgcggcga
     4141 ctcggcctgc cggtcgcgga gaggcccgac agtcaggatt tcatcgcggg cgactacggg
     4201 atactcttct cgcccgaaga ggcgccgccc ggcgatatcg tgaacgaaaa gggggcgctg
     4261 ctcggccgcc accgcggcat cgtgcactac accgtcggcc aacggcgcgg gctcggggtc
     4321 gacggcggga aaccgctcta tgtgaccgcc atcgacgccc gcaacaatcg catcgtcgtg
     4381 accgacaagg atcatctctt cgggcgggga ctggtggccg gtcaggtgaa ctggctgggg
     4441 atggaccggc ccgaccgccc gttccgggcc catgcccgca tacggcagaa acactccgag
     4501 gcgccggcga cggtgatccc tcgcgacgac ggcacgataa cggtgcggtt cgacgaaccg
     4561 cagttgtcga tcaccccggg acaggcggtg gcgctgtacg cggacgatac ggtgctcgcc
     4621 ggtgcgacca tctctcagag aacagacgag ggagccgata catgaaacgg atctgctggg
     4681 gattctgcct gatcctttta gccgcaatgc cgctcgcggc cgatggaccg gaacagccgc
     4741 agggaccgga gaaggaccgg cctgccgcgg aagaaaaggg cgacgagccg gtcgccgacg
     4801 gcggtcgggt ctacctcaag gtgggcggcg gcctgctgtt cgacgagcgg cgcgttgtcg
     4861 tcgagccggc cttcgggttc ggctaccgct acacgcaggg cgcattcgcg gtcgatctgt
     4921 cggtattcaa cgaaacgatc ggccccaccg tcgactacgc cggctataag gaagagcagg
     4981 aatacgagcg caacgccctg cgcatcacga tgtcccgctg gatcgggcta tccttcctgt
     5041 gggtcgccga gccgcgtgca tggaacagtt tcttcgctgg cggcgggctc gcgatagaga
     5101 cgctcgacct cgaactggcg agcgagtcgt cgtcgcagac cgggctcggc gtcactctcg
     5161 tcgccggctg ggaattcctc cgcgaccgga cggtgatact cttcgcgcag gcggaggtcg
     5221 atcttccgtg ctataccgcg cggttctatg gcgaccgctt ctgggtcccg tccatcgtat
     5281 tcgtcatcgg cgccggtctc taacgccgga gcgaccgccg gtccccgaaa accttgacag
     5341 cccttgagag ataggtaata tcggcaacca ttggtgccca ggtagctcag tcggtagagc
     5401 gagggactga aaatccctgc gtgggcagtt caattctgtc cctgggcacc agtatttcct
     5461 caaaagacct ttttccgtga ccgctttgca atgcgcatcg cttcgtggta tggtgtttcc
     5521 gtcccgatca cccgtgcagg gaggcaccga tgaagcgaca gatagtggcg ctccttatgt
     5581 tcctgacggc gacgaccgcc gcggcaggcg acatcccctg gtcgaacacc gataccggcg
     5641 cgatagccga gcgcatcccc gaactgtcgg ggttctccgt tgacggcgtt gcggtcgacc
     5701 tcccccggcg cctcctgacg gtacgcctga ctctcccggc ctctcttacg ctcgatgaac
     5761 gcatcctcga cgagatgaac cgtctcctcg tcagcatgct cgcgcagaag aaactgccgt
     5821 tccggacgct cgtcatactg gcgcgccgca ccggccagac cgagtggcgc gacatccgct
     5881 ccctgctccc gacactcccg ccggtgccgc gcaaggagca cgaaccggtc gcctcagaac
     5941 ctaatccgta cctcggtttc ccgagatcgg gcacgaccgg caccgccggg gcgctcgccg
     6001 ggaagacggt ctatctgagc gccggtcacg gctggtacta caacgggagc gcatggacga
     6061 cccaacgcgg caacacggtc ggcatcgtcg aggatctttc caacaccgag atcatatcct
     6121 actacctcgc tccgatgctc cggaacgctg gcgctctcgt cttcacggcg cgggaaagcg
     6181 accgcaacgc cgtgtcggtc acggtggatg acgctgacgc gaccaacctg ccggcgaacg
     6241 gggcttacga ggaatcggac gggtggaccg aatcgaccgg taccggctgg ggacggctga
     6301 caacgccgtt cgcgaccggc gacgccccct tttcggcggg ccattaccgc gtgaccgagc
     6361 cagcgaccgg tcgctgggcg cgctggacac cgaatctgcc gaccggcgga cgctatcaga
     6421 tattcgtctc tttccgcccc atgagcgacc gcgctccgac ggcgcgctac gaggtgcgcc
     6481 acgccggcgg catgacgacg gtcgtcgtcg accagcggca tcacggcaac acctggttcc
     6541 cgctcggcga attcaccttt ttcggcggct acagcgtcga taaggggtcg gtcaccctcc
     6601 tcgccgatac gacggcgggc gggtcgtatg tcatcgccga cgcggtccgg ttcggcggcg
     6661 ggaccggtat catcgaccgc gacggcggtg tttcaggaag accgcgctgg gaagaggcgg
     6721 cacgctacca cgcccaactg ctcggcgctc ccgcgaccgt ttacgacacc tccgataccg
     6781 acaattccga cgatgtgacc acccgatcgc gctgggcggc gtgggagaac gagtcgggct
     6841 gggacgatag cgtattcatc tcatggcatt cgaacgccta caacggtaac gcgcgcggca
     6901 cctcgaccta tgtctacggg ccgaacgacc ccggcaccgg ctgggaaccg gcggcgcagg
     6961 agggaagcga ggaactcgcg acgcttctcc acaacggcgt ggtcgccgcc ctgcgtgacg
     7021 gatgggacgc cgcatggccc gtgtcgggct ccggacttta cagcgcgtgg ttcggcgaac
     7081 tcaatcccag ttacaacaac gagatgcccg cctgtctcat cgaactcgcg ttccacgaca
     7141 atacgaccga ctcgaacgcc ctacgcgacc cgcgcttccg gctcatcgcg gcccgggccg
     7201 cgacgcaggc ggtgatacag tacttcgccg accgcgacac gctggaggcg gtctatccgc
     7261 ccgacacgcc gcggatggtg cggacgaccg cgatcgggcc gcacgaggcg atcatatcgt
     7321 gggaggcgcc gcccgattcc ccgcgcaccc tctacggtga tgcgcctacc ggctacatcg
     7381 tccagcgaag cgccgacggg ttcgcgttcg atgacggcgt cgaggtcgag ggagcgacct
     7441 cgttcacggc acaggacctg tatccctact ccgtcgacta tttccgggtg atcgcctaca
     7501 acgacggcgg tcgctccttc ccctcgccgg tcgtcgcggt gcgtccgaaa agcgacgggg
     7561 cgcaggtgct catcgtcgac ggcttcgagc gactcgaccg tggtctgctc gtctcgcagg
     7621 acctttcttc ctacggtctc ggtgccgtca tgcggatgtt cctcgagcgg atgaacacct
     7681 tcgactatgt ccgcgcccac gctctggcac tcagcgacct ttcctattcg ttcgattcgg
     7741 tgcagaaaag cgccctcgag accatcgatc tcactgtgta cgacgcggtg ctttggttca
     7801 ccggcgaaca gtcgaccgcc gatgtgacgg taaccccggc cgaacagacg cacctgtgcg
     7861 actatctcga cggtggcggg atactcttca tctccggctc cgagtacggg tgggaccttt
     7921 accacatggg cgacgcgacc gatcaggcct ttttcaccga ctgtctcgga ctctcgtatc
     7981 tgggcgacga tgccgggacc tatcaggcgg cgaccgtcac cggcggcgag gatgtgttca
     8041 acggctcgct ctcgttcgac gacggctctt tcatctataa tgcggattac cccgacctca
     8101 tcgaggcggc gggcgacggc atcaccttcc tcggttacgg tgacggcacc ggcggcggcg
     8161 cgggggtcgt caccggcggt gaccggaagg tcgcggcgct cggattccct ttcgagacga
     8221 tactcgacgc cgacacgcgg cggacgcttc tgggcggcat cttggcttgg tacgacctga
     8281 cggctctcga ggagccggtc gacgaggatg tgatcgagag cgacaacgaa atctccgacg
     8341 accacgcggc accggtcgat gacggggatg ccgaccccga ctatggcggc gggtgcgccg
     8401 aaggcgatta ttccttcagc gatgaagatg caacggtcgt cgatgaaacg acggttgatg
     8461 aagacaccgt attttctgat gatcagtcgg atcagtcgga tcagccggat caggccgacc
     8521 agccggatca gaccgatcag ccggatctgt acggcctccc cgacgaaacg acggtcgacg
     8581 aactgatcat cggtgaagac agcgacactg caaaaggttc ttcaagcggt tgcggctgtt
     8641 cggtgatcta ggtcagaagt acagttcgta ttcctgcggc gtcggcgcgt tgtagacatg
     8701 ggtcgcctcg tcgcgtttta tctttatcca caggtcgagc aactgttccg ggaacgcggg
     8761 gagcaggtac cggcggtcct tctgcaggcc atcgagcacg aggttgagat cgagcgggaa
     8821 cgacttctcg ttcttcatgt cggagaaacc cagtttcacc ggatcggctt tgcgccggat
     8881 accgtcgatc cccgcgagca ccatcgccgc aagcatatag taggtattcg ccgtcgcgtc
     8941 gccggtacgg aactctatgc gaacatcctt tcccttcagg tagccgggga tgcggatcgc
     9001 cgcagcgcgc gaggccttcg cgaaggtcgc gttgaccgga gcctcgaagc ccggcacgag
     9061 ccgtttgtag gagttggtcg acgggttggt gagcgcgaga agcgacccgg tcagcgaatg
     9121 ttcgagtatg ccgcacagat acgaatgggc gagcggactc aggttataca gagccttgcc
     9181 ggggaagagc gactcgccgc ccttttcgag gaactggtgg acatgcatgc cggagccggc
     9241 gaccttgtag atcggtttgg gcatgaaggt gacgaacagg ttgtgctcgc gcgcggcgct
     9301 cttgatgatc cacttggcga gcgtcacccc gtcggccgcc tcgaggagcg gcaggaagtt
     9361 gagttctatc tccagttgcg cggcgccgac ctcgtggtgg tggtacttca ccgggatgcc
     9421 gatcgcctcc atcgtcgcga ccaccgtatt gcgaagcgcg aaatgacggt cgtccggcgg
     9481 ctggcggtga tagccgcggt ggatgccgaa acggggctgg tcgtagtatt cctcgccgat
     9541 cccctcggga ctctgcacct tgtaataact gtgtccgaaa tcggtcgcgt aggagacctc
     9601 gtcgaacaca tagaactcca attccacaag cattttggcg gtgtcggcga tcttctgctt
     9661 tttcaggtga tcgagcgtgg cgcggaccac attgcgcgga tactgcgcga agggtcttcc
     9721 gtcgg
//
LOCUS       DAOQUE010000042         9499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8053_length_9499_cov_4.257200, whole genome shotgun sequence.
ACCESSION   DAOQUE010000042 DAOQUE010000000
VERSION     DAOQUE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8053_length_9499_cov_4.257200"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..>443
                     /locus_tag="PKH10_02285"
     CDS             237..>443
                     /locus_tag="PKH10_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26984.1"
                     /translation="MRSLLLLMLSLVVLVACEDGTRRIIPLDTEAVADGDVIVTGDED
                     MLLVEGADDPLNDDGVPVNDDGVPV"
     assembly_gap    444..543
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <544..1485
                     /locus_tag="PKH10_02290"
     CDS             <544..1485
                     /locus_tag="PKH10_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26985.1"
                     /translation="NDDGVPVNDDGVPVNDDGVPVNDDGEPVNDDGEPVNDDGEVTPD
                     IDIDNAPCSPNDTRQIPCGINGAGLQSQICIGSDWQNQGICVDDDVCKNGEEQDIACG
                     INNDGIQSQDCVDGQWVNDGACVDDDVCENGTEQTIPCGDGGKGDQAQDCVDGQWVND
                     GDCVMPSQTGRWTCNGSKQCVPEYGYAACGNGTCDPKNGESAKSCPADCDKAAVNGKD
                     QNCSDIYDCAFYGWFESTPGYWGCGGYPKKCTVNKSTTYCDKTGYDYCYAGTTVGIET
                     DKTCSADCANTMLNQNAQTGCNNPVDCIFLDWPENNG"
     gene            complement(1492..2910)
                     /locus_tag="PKH10_02295"
     CDS             complement(1492..2910)
                     /locus_tag="PKH10_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT26986.1"
                     /translation="MNAPTPIPREEILHGRTLPLMIRLGWPVMLSSLLDTMYQLTDTF
                     WLGRLAGTESGTAVASLQVAFPFLWFLVAFFADFSVAGTALVSQHTGAGDKRTANEAA
                     GQVLSVVIIGGTLAALAGMLLFPVVMPLITDNAAIRDTARSYLLWYLPGLPFVFITAV
                     FRVLMAAAGNTFVPLMVVFTGNVINMALDPLFILGLGPVPAMGVQGAAIATVISSFIA
                     GLIALRLLMNGRNAIRLTFGDLVPRLHWIRRILKIGLPAAVGDSAEALGFVVLMALIG
                     RVESAREALGGYGIGNRLTSLIAIAIVGLGQGLSTIIGQNLGASRFDHARRAAGQGIA
                     ILFGVLAVETGIIILFREPLVRLFIPNDPGVIAEATRFLLYFGSSVPLFGLIRGITAA
                     FNAAGRNAPVLVIGLLRLWILRLPLAWLFAFGLGYGTPGLWFGMALSNFGAAIAAIIF
                     FTRFDWKRNLIEPTPGPEQAPE"
     gene            complement(2907..3365)
                     /locus_tag="PKH10_02300"
     CDS             complement(2907..3365)
                     /locus_tag="PKH10_02300"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005450628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNT26987.1"
                     /translation="MSREILSGLFLEVTASGRLLAATRITARPIDSGVTAMLDRIVDR
                     FLGRWRHGIHDPLDDDLFLFPETLDKWAPLLLRLKNTVGPGMTVTYGELGGPLGIHPR
                     TVGLVMARNPFPLLIPCHRVVGADGRLTGFSAEGGIDTKQRLLAAEMNPR"
     gene            complement(3355..4020)
                     /gene="pgsA"
                     /locus_tag="PKH10_02305"
     CDS             complement(3355..4020)
                     /gene="pgsA"
                     /locus_tag="PKH10_02305"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNT26988.1"
                     /translation="MKLRKKKEKANRNGFRRPKIFSKLRDFKKDVLTLPNLLTTVRIG
                     AVPLILWLLYQDSASSRIVAAIVYAIAGVTDYLDGYLARKYGEVTVTGKLLDPLADKF
                     IVLLTLVVLLDKHEVALLPVLLILAREFYIFGIRSIAVEHGLVISAGSGGKWKTTFQM
                     FAIPFLIITPSTTFALFGAEWDLPYWGNILLWISVFFALQSAAVYTLTLKRRLFESTH
                     HEP"
     gene            complement(4017..4646)
                     /locus_tag="PKH10_02310"
     CDS             complement(4017..4646)
                     /locus_tag="PKH10_02310"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNT26989.1"
                     /translation="MTGTGTANSSRRKRASLIALTLLGALTLTAQAKIYYYYDPINDS
                     YTFTNMCGDLRLCKPLFEEKGRVTHTKGFAYKPGMDTKYDSHIRAAADRYGLDFHLVK
                     SVIKAESQFDANARSSKGAQGLMQLMPDTARLLGVKNAYDPEQNILGGTRYLRDMLKK
                     FRNAEKALAAYNAGPAAVVYHDGVPPYQETKNYLVKIRRFYREYTGKDL"
     gene            complement(4600..5952)
                     /locus_tag="PKH10_02315"
     CDS             complement(4600..5952)
                     /locus_tag="PKH10_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26990.1"
                     /translation="MWDKISPYVNTGKKLKIKGDKLIAILLQNRFQGTLFGHAAMGTD
                     EIVFKDDQIYSSQSPSYYTFALQNALFSLASAAAETKATGRNGYEFLFEVVKGYPADM
                     LQKMLEPYKTENITMKPEVAQFSFLREALEPYAGKQSFSVVSLFMEQIHTLYFLLLTE
                     QAAVLKASAAEREYATEEEKRRAEEEKLQKDRATISMSEEWLDLDRYVKRAFKYESVF
                     SLFSWKEGDDLGGAHKIYLGFVQKLHPDRIQNAPERLREKANELLAVINSVYPLLKDD
                     ELRAKMLALTKKYGNIRTKERYEELLQVDELIIRADALFKLGEAGKAYKIYVEMYGRT
                     KVSWLLEKMIYAYYHNRRPEEDAAKGIRDGRRVTPEGKYMKLRDYIQRLRDFKSALPL
                     EILYILAEVYEKEHNIVMLRKTLQEILVLYPGESKAIGWLKKVDDWYRNRQQQQKKEG
                     "
     gene            complement(5981..7645)
                     /locus_tag="PKH10_02320"
     CDS             complement(5981..7645)
                     /locus_tag="PKH10_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HNT26991.1"
                     /translation="MTRKLLIRDLTLRDGQQSQFATRMSQRQVDRVIALFQKAHFYAM
                     EVWGGAVPDSIMRYLDENPWTRLESIKAGIGDVSRLTALSRGRNLFGYNPYPDEVIEG
                     FCRNAVKSGIGIMRIFDALNDLSNQASTIRFVKENGGLADCCVCYTVDPHFTRGDRVR
                     ALFSGKKLPSPIFTVDYFVAKARELEKMGADIISVKDMAGLIHPKTAGELTRGLKGAV
                     SVPIDLHTHCTPGYGLGSVLMAMVNGADIVDTVIMPFAGGPAAPAFELIQIFADKLGI
                     DTGVDLSVISDLQEELLAIRHELCGFDQYKLFPRLINPADYTLEPELNALFDAAIAAA
                     KKDDIPEVLRTTQAIERHFNFPAPDEMVRVAQVPGGMYTNMLSQLQQADLGHLLKEVL
                     ASIPQVRLDAGLPPLVTPTSQIVGIQAVNCIIDRNAGKPFYTNKSIQFVNLVKGVYGK
                     TPFPVDPAFRLKIAGTREEIPYDTAAHKKEENPTLPEYGGVRLANGEKEELLLELFPQ
                     VARNYLKKVREREWIERMERERDAQQQAADSANLPIFEGLRGNIIV"
     gene            complement(7671..8795)
                     /locus_tag="PKH10_02325"
     CDS             complement(7671..8795)
                     /locus_tag="PKH10_02325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT26992.1"
                     /translation="MKIALLGPDPSFKGGIGQYNHFLAEEILKEGHELLFIGFLRQYP
                     KFLYPPGTGDGAGTGPERHYTVERILSWDRPFSWVTAARHIEAFSPDVVVVPWWTFFW
                     YPWFAPLLNRLRNFKRVVIAHNASDHEDHGPRGAASRFATRRILATADRVIVQSVEEK
                     RHVAALGARSVAVVHHPVYPQFRSTVSASDARASFGIAPDAKVALLFGAVRPYKGADL
                     FAGAIAALPDITGIIAGELWDGALATRLRELAAAHPNLRLLDRYFDDDAGATLFAACD
                     VAVLPYRSVTGSGAAMAALGAGRPIIASDLPLFREILPAPLARSFAAGDVVDLSRALR
                     EFFSENNKLQSEEALAIICGNFGWDRLVREVSTAATAPTD"
     gene            complement(8770..>9499)
                     /locus_tag="PKH10_02330"
     CDS             complement(8770..>9499)
                     /locus_tag="PKH10_02330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT26993.1"
                     /translation="IELDADLIVNTDADNQYKGACIPELVRPILEKRADMVVGVRPIG
                     DIEHFSVVKKILQRIGSAVVRLASGTRVEDAPSGFRALSREAALRINVFSDYSYTLET
                     IIQSGLKNIVVATVPVEVNPPTRPSRLFRSTTGYVLRSLTTIARIFILYRSFRFLALS
                     GGIIFGVGFVLGLRFLYYLIEGTGDGHIQSLLLGAVLLIAGFQTFLIGIIADLIAANR
                     KMLEEIQYLQRKGSARHEDSAPRS"
BASE COUNT         1768 a         2700 c         3109 g         1822 t
ORIGIN      
        1 tagtcgccga tgatggcgcc ctcgtcggcg cggcgcagga tgtcggtggt cagggccctg
       61 aaatcgctgt gggatgttct tttgcgccgc gcgggcatgc tctcctccgg ctcgaccgaa
      121 gcgatgataa ccaatcgaat gaggaacaca aataattaag aaagtgcttg aattcccgtc
      181 gtcgttgcgt agaattcgag accgttgaca tacgatacga tcgtcaggag gttgccatga
      241 gatcgctgtt gctccttatg ctttccctcg tggtgctggt cgcctgcgag gacgggaccc
      301 gccgcatcat cccgctcgat acggaggcgg tcgcggacgg ggatgttatc gtaacgggcg
      361 atgaggatat gctccttgtc gagggggccg atgacccatt gaacgacgac ggcgtaccgg
      421 tgaacgacga cggcgtgccg gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnaacgacg atggcgtacc ggtgaacgac gatggcgtac cggtgaacga cgatggcgta
      601 ccggtgaacg acgatggcga gccggtgaac gacgatggcg agccggtgaa cgacgatggc
      661 gaggtcacgc ccgacattga tattgacaat gccccctgtt ctcccaatga cacgcgccag
      721 ataccgtgcg gcatcaacgg cgctggactc cagtcgcaga tatgtatcgg cagcgactgg
      781 cagaatcagg gcatctgcgt ggatgacgat gtctgcaaga acggcgagga acaggatatc
      841 gcctgcggca tcaataacga cggcatccag tcgcaggact gcgtcgacgg ccagtgggtg
      901 aacgacgggg cctgcgtgga cgacgatgtc tgtgagaacg gtacggaaca gacgataccg
      961 tgcggcgatg gtggtaaggg cgatcaggcg caggactgtg tcgacggcca atgggtcaac
     1021 gacggcgatt gcgtcatgcc gtcgcagacc ggtcgctgga cctgtaacgg cagcaaacaa
     1081 tgtgtgccgg agtacggata tgcggcgtgc ggtaacggca cctgcgaccc gaagaacggc
     1141 gagtcggcca agagttgccc cgccgactgc gacaaggcgg ccgtgaacgg caaggatcag
     1201 aactgctcgg atatctatga ttgcgccttc tacggctggt tcgagagcac tcccggctac
     1261 tggggctgcg gaggctatcc gaaaaaatgc accgtcaaca agagcaccac ctactgcgat
     1321 aagaccggat acgactattg ttatgccggg accacggtcg gtatcgagac cgacaagacc
     1381 tgtagtgccg actgcgccaa cacgatgctc aaccaaaatg ctcagaccgg ctgtaataat
     1441 ccggtcgact gcatcttcct cgactggccc gagaacaacg gctgatcccg gctattccgg
     1501 cgcctgttcg ggccccgggg tcggttcgat aagattcctt ttccagtcaa agcgcgtaaa
     1561 gaagatgatg gcggcgatcg ccgcgccgaa gttggaaagc gccatgccga accagagtcc
     1621 cggcgtcccg tagccgagcc cgaaggcgaa cagccacgcg agcggcaggc gcagtatcca
     1681 gaggcgcaga aggccgatga cgagcaccgg ggcgttgcgg cccgcggcgt tgaaggcggc
     1741 ggtgatgccg cggatgagcc cgaagagcgg caccgaactg ccgaagtaga gcaggaaccg
     1801 cgtcgcctcg gcgatgaccc ccggatcgtt cgggatgaac aggcgcacga gcggttcgcg
     1861 gaacagtatg atgatccccg tttcgaccgc gaggacgccg aagagtatcg cgatgccctg
     1921 tcccgcggcg cggcgtgcat ggtcgaagcg ggaggcgccg agattctgcc cgatgatggt
     1981 gctgagcccc tgcccgagcc cgacgatggc gatggcgatg aggctcgtga gccggttgcc
     2041 gataccgtag ccgccgagcg cctcgcgggc cgattcgacc cgtccgatga gcgccatgag
     2101 gacgacgaac ccgagcgctt cggccgaatc gcccaccgcc gccgggagcc ctatcttgag
     2161 tatgcgccgt atccagtgaa ggcgcggcac gagatcgccg aaggtcagac ggatggcgtt
     2221 gcgaccgttc atgaggagcc ggagcgcgat gagcccggcg atgaacgatg atatgaccgt
     2281 ggcgatcgcc gccccctgca cacccatggc gggtaccggg ccgagtccga ggatgaacag
     2341 cgggtccagc gccatgttga tgacattgcc ggtgaatacg accatgagcg gcacgaaggt
     2401 gttccccgcg gccgccatga ggacgcggaa caccgcggtg atgaacacga acgggagccc
     2461 cggcaggtac cagagcagat aggagcgggc cgtgtcgcgt atcgcggcgt tgtcggtgat
     2521 gagcggcatg acgaccggga aaaggagcat gccggcgagc gccgcgagcg tgccgccgat
     2581 gatgacgacc gaaagcacct gtcccgccgc ctcgttcgcg gtccgtttgt cgcccgcacc
     2641 ggtgtgttgg gaaacgagcg cggtgccggc gaccgagaaa tcggcgaaga aggcgacgag
     2701 gaaccagagg aacggaaagg cgacctgcag ggaggcgacg gcggtgccgc tctcggtgcc
     2761 ggcgagccga ccgagccaga aggtgtcggt aagttgatac atcgtgtcga ggagcgacga
     2821 gagcatgacc ggccacccga gccggatcat gaggggtagg gtgcgaccgt gcagtatctc
     2881 ttcgcgcggg atcggcgtcg gtgcgttcat cgggggttca tctccgcagc gagcaggcgt
     2941 tgtttggtgt ctatgccccc ttcggccgag aagccggtca gccgtccgtc ggccccgacg
     3001 acgcggtggc acgggatgag gagcggaaag gggttgcgcg ccatcaccaa gccgacggtg
     3061 cgcgggtgta tgccgagcgg gccgccgagt tctccatagg tgacggtcat ccccggcccg
     3121 accgtgttct tgagtcgtaa aaggagaggt gcccacttgt cgagcgtttc gggaaaaaga
     3181 aagaggtcgt cgtcgagcgg gtcgtggatg ccgtggcgcc agcgaccgag aaagcggtcg
     3241 acgatgcggt caagcatcgc ggtcacgccg ctatcgatgg gccgcgcggt gatgcgggtc
     3301 gcggcgagca gtcggccgga ggcggtcacc tcgagaaaga ggcccgaaag tatctcacgg
     3361 ctcatgatgg gtgctttcga acaggcgtcg cttgagcgtc agggtgtaga ccgccgccga
     3421 ttgcagggcg aaaaagacgc ttatccacag gaggatgttc ccccagtagg ggaggtccca
     3481 ttcggcgccg aacagggcga aggtggtcga gggtgtgatg atgaggaacg ggatggcgaa
     3541 catctggaag gttgtcttcc acttgccgcc cgatccggcg gagataacga gcccgtgttc
     3601 gaccgcgatg ctcctgatgc cgaagatgta gaactcacgg gcgaggataa gaagcaccgg
     3661 aagcagggcg acctcgtgtt tgtcgaggag caccacgagc gtcagcagga cgatgaactt
     3721 gtcggcgagc ggatcgagca gtttgccggt cacggtgacc tcgccgtatt tccgggcgag
     3781 atatccgtcg agatagtcgg tgacgccggc gatggcgtag acgatcgcgg ccacgatgcg
     3841 ggacgacgcc gagtcctgat agaggagcca gagtatgagc ggtaccgcgc cgatgcggac
     3901 ggtggtgagc aggttcggca gggtcagcac atccttcttg aagtcgcgca gtttggagaa
     3961 tattttggga cgccggaagc cgttccggtt cgccttctct ttcttttttc tcagtttcat
     4021 aggtccttgc cggtgtattc tcgatagaag cggcggatct tgaccagata gttcttcgtt
     4081 tcctgatacg gggggacacc gtcatgatag acgaccgccg cggggccggc gttgtaggcg
     4141 gcgagcgcct tttcggcgtt cctgaatttc ttcagcatgt cgcgcagata gcgtgtgccg
     4201 ccgaggatgt tctgttcagg gtcgtaggcg ttcttgaccc ccaacagtct cgcggtatcg
     4261 ggcatcaact gcatgagtcc ctgcgctcct ttcgatgaac gggcgttcgc gtcgaactgg
     4321 gattcggcct ttatcacact cttcaccaga tggaaatcga gtccataccg atcggccgcc
     4381 gcgcgaatgt ggctgtcgta cttcgtatcc atacctggtt tgtaggcgaa gccctttgta
     4441 tgggtcacac gccccttttc ttcgaacagc ggtttgcaga gtcgcagatc gccgcacata
     4501 ttggtgaagg tgtaggaatc gttgatcggg tcgtagtaat agtatatctt cgcctgcgcc
     4561 gtaagggtca gcgcgccgag aagcgtcagg gcgatgagac tagccctctt tcttctgctg
     4621 ctgttggcgg ttccggtacc agtcatctac ctttttcaac catccgatgg ccttgctctc
     4681 tccgggatag agtacgagta tctcctgcag ggtcttgcgc agcatgacga tattgtgctc
     4741 cttctcgtag acctcggcga gtatgtagag tatctcaagc ggaagagccg atttgaaatc
     4801 gcggagccgc tgtatgtagt cgcgcagttt catgtatttg ccttcggggg tgacgcgccg
     4861 cccgtcccgg atgcccttcg ccgcgtcttc ctcggggcgg cgattatggt agtaggcgta
     4921 gatcatcttc tcgaggagcc acgacacctt ggtccgaccg tacatctcga catagatctt
     4981 gtaggccttg cccgcttcgc cgagtttgaa cagcgcgtcg gcgcggatga tgagttcatc
     5041 cacctgcagg agttcctcat agcgctcctt ggtgcggatg ttgccgtact tcttggtgag
     5101 cgcgagcatc ttcgcgcgca gttcgtcgtc tttcaggagc gggtacacac tgttgatgac
     5161 cgcgagcagt tcgttcgcct tttcgcgcaa ccgttcgggt gcgttctgga tgcggtcggg
     5221 gtgcagtttc tgcacgaagc ccagatatat cttgtgcgca cctcccagat cgtcaccctc
     5281 cttccaactg aagagcgaga agaccgactc gtacttgaag gcgcgtttga cataccggtc
     5341 gaggtcgagc cattcctcgg acatggatat cgtggcgcgg tccttttgga gtttctcctc
     5401 ttcggcgcgc cgcttctcct cttcggtcgc gtactctcgc tcggcggcgc ttgccttcag
     5461 gaccgccgcc tgttcggtga ggagcaggaa atagagggtg tgtatctgtt ccatgaacag
     5521 cgaaacgacc gagaacgact gtttcccggc atagggttca agcgcctccc gcaggaagga
     5581 gaactgcgct acctccggtt tcatcgtgat gttctcggtc ttgtagggtt ccagcatctt
     5641 ctggagcatg tcggccgggt agcccttgac cacctcgaaa aggaactcgt agccattcct
     5701 gcccgtcgct ttggtctcgg ccgcggcgct cgccagcgag aagagggcgt tctgcagggc
     5761 gaaggtgtag taggagggcg actgggatga gtatatctga tcgtccttga agacgatctc
     5821 gtcggtgccc atcgcggcgt gcccgaagag cgttccctga aaacggttct gaagaagtat
     5881 ggcgataagt ttgtcgccct ttatcttcag ttttttcccg gtgttcacat acggagagat
     5941 cttgtcccac acggtccctt cccccagact atccttttct tcagacaatg atgttgcccc
     6001 gtaacccttc aaatatcggt agattcgcac tgtcggcggc ctgctgctgc gcatcccgct
     6061 cccgttccat ccgctctatc cattcgcgtt cgcgcacctt tttcagataa ttccgcgcca
     6121 cctgcgggaa cagttcgagg agcaactcct ccttttcgcc gttcgccagc ctgacgccgc
     6181 cgtattcggg cagggtcgga ttctcctcct tcttgtgcgc cgcggtatcg taaggtatct
     6241 cctcgcgggt cccggcgatc ttcagccgga acgcggggtc gaccgggaac ggggtctttc
     6301 cgtacacacc tttgacgaga ttgacgaact gaatggactt gttggtgtag aacggtttcc
     6361 cggcgttgcg gtcgatgatg cagttgaccg cctgtatgcc gacgatctgg cttgtcgggg
     6421 tgacgagcgg cggtaggccg gcgtcgagcc gcacctgcgg gatgctcgca agcacctcct
     6481 tgagcaggtg gccaagatcg gcctgctgga gttgcgagag catgttggtg tacatgccgc
     6541 ccggtacctg tgcgacgcgg accatctcgt cgggggcggg gaagttgaaa tggcgttcga
     6601 tcgcttgcgt ggtccgcaac acctccggta tgtcgtcctt tttcgccgcc gctatcgccg
     6661 cgtcgaacag cgcgttcagt tcgggttcca gcgtgtaatc cgccgggttg atgagccggg
     6721 ggaagagttt gtactggtcg aacccgcaca gttcgtggcg gatggccagc agttcctcct
     6781 gcaggtcgga tatcaccgag agatcgacgc cggtgtcgat ccccagtttg tcggcgaata
     6841 tctgtatcag ttcgaacgcg ggtgccgccg ggccgccggc gaacggcatg atgacggtgt
     6901 cgacgatgtc ggcgccgttg accatcgcca tcagcaccga cccgagtccg tagccggggg
     6961 tgcagtgggt atgcaggtct atcggcaccg acacggcgcc cttgaggccg cgcgtcagtt
     7021 cccccgccgt cttgggatgg atgagtcccg ccatgtcctt gaccgatatg atgtctgctc
     7081 ccatcttttc gagttcccgc gccttcgcga cgaaatagtc gacggtgaat atcggcgagg
     7141 ggagtttttt accggaaaag agcgcccgca cccgatcgcc gcgggtgaag tgcgggtcga
     7201 cggtgtagca gacgcaacag tcggccagcc cgccgttctc cttgacgaaa cgaatggtcg
     7261 aggcctgatt cgacaggtcg ttgagcgcgt cgaagatgcg catgatgccg atgccgctct
     7321 tcacggcgtt gcggcagaat ccctcgatca cttcgtcggg gtagggattg tagccgaaca
     7381 ggttgcgccc gcgcgacaat gccgtcagtc tcgatacgtc gccgatgccg gccttgatcg
     7441 attcgagccg cgtccacggg ttctcgtcga gatagcgcat gatcgaatcg ggtaccgcgc
     7501 cgccccagac ctccatggcg tagaaatggg ctttttggaa gagtgcgatg acccggtcga
     7561 cctgccgctg actcatgcgg gtggcgaact gcgattgctg accgtcgcgg agcgtaagat
     7621 cgcggataag gagtttgcgt gtcatggacc cgatatgcct cgtcgttgtt tcaatccgtg
     7681 ggggcggtcg ccgcggtcga gacctcccgt accagccggt cccagccgaa atttccgcaa
     7741 atgatagcaa gagcctcttc cgattgcaat ttattatttt cggaaaagaa ctcgcgaagg
     7801 gcgcgggaaa gatcgacaac gtcaccggcg gcgaaggatc gggcgagcgg cgcgggaagt
     7861 atctcgcgga acaacggcag gtccgacgcg atgattggtc ggcccgcccc gagcgccgcc
     7921 atcgccgctc ccgagccggt gacgctccgg taggggagca ccgcgacatc gcaggcggcg
     7981 aaaagcgtcg cccccgcgtc gtcgtcgaag tagcggtcga ggaggcggag gttggggtgc
     8041 gccgccgcga gttctcgcag acgggtcgct agagcgccgt cccacagttc ccccgcgatg
     8101 atgccggtga tgtcgggcag ggccgcgatc gccccggcga acagatcggc ccccttgtag
     8161 ggacggacgg cgccgaacag gagcgccact ttcgcgtcgg gcgctatgcc gaaagaggcc
     8221 cgcgcatcgg aagcgcttac cgtcgaacgg aactgcggat agacggggtg atggacgacg
     8281 gcgaccgacc gcgcgccgag cgccgccacg tgtctttttt cctcgaccga ctggacgatg
     8341 acgcggtcgg cggtggcgag tatgcgtcgg gtggcgaatc gcgacgcggc tccgcgcggg
     8401 ccgtggtctt catggtcgga ggcgttgtgg gcgatgacga cccgcttgaa gttgcggagg
     8461 cggttgagga gcggcgcgaa ccacgggtac cagaaaaagg tccaccacgg tacgaccacg
     8521 acatcggggg agaacgcttc gatgtgccgc gccgccgtca cccacgagaa aggacggtcc
     8581 cacgacagaa tgcgttcgac ggtgtagtgc ctctccggac ccgttccggc accgtcaccc
     8641 gttcccggcg ggtagaggaa cttcggatac tgccggagga acccgatgaa gagaagttcg
     8701 tgtccctctt tcagtatctc ttcggcgagg aagtggttgt actggccgat gccgcccttg
     8761 aacgacggat caggaccgag gagcgctatc ttcatgccgc gcggatccct tccgttgcag
     8821 gtactgtatc tcctcgagca tcttgcggtt cgccgctatg aggtcggcga tgatgccgat
     8881 gaggaaggtc tggaacccgg cgatgagaag caccgcgccc agcaggaggc tctgaatgtg
     8941 tccgtcgccg gtgccctcaa tgaggtagta caggaaccgg agcccgagca cgaagccgac
     9001 gccgaagatg atgccgccgc tcagcgcgag gaaccggaac gaacggtaca ggatgaatat
     9061 ccgtgcgatg gtcgtgagcg atcgcaggac atacccggtg gtcgaacgga agaggcgcga
     9121 cggccgggtc ggcgggttga cctccaccgg caccgtcgcg acgacgatgt tcttgagccc
     9181 gctctggatg atggtctcga gcgtatagga atagtcggag aatacgttga tgcggagcgc
     9241 cgcctcgcgg gacagcgcgc ggaacccgct cggggcgtcc tcgacccgcg tgcccgaggc
     9301 gagccgcacc actgcgctcc cgatcctctg caggatcttc ttgaccaccg agaaatgctc
     9361 gatgtcgcct atcgggcgga cccccaccac catgtcggcc cgtttttcga gtatcgggcg
     9421 gaccagctcg gggatgcacg cgcccttgta ctggttgtcg gcgtcggtgt tgacgatgag
     9481 gtcggcgtcg agttcgatc
//
LOCUS       DAOQUE010000043         9360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8204_length_9360_cov_4.702418, whole genome shotgun sequence.
ACCESSION   DAOQUE010000043 DAOQUE010000000
VERSION     DAOQUE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8204_length_9360_cov_4.702418"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /gene="yihA"
                     /locus_tag="PKH10_02335"
     CDS             <1..582
                     /gene="yihA"
                     /locus_tag="PKH10_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNT26994.1"
                     /translation="PPGPAPPMIEIRTATFLKSAAHPADLPPPTLPEYAFIGRSNVGK
                     SSLMNLLLGRRNLVKTSKTPGKTVTMNFFDINGAFRFVDLPGYGFAKRSKSEQEVWRR
                     TIESYLTRRDNLRLILLLIDGRHGPQPNDEQMIAFLDHHRLPWLPVFTKIDKLSTAEK
                     QRLRRGNPGAPCVSATTGEGKEETWAHIERYWG"
     gene            552..1091
                     /locus_tag="PKH10_02340"
     CDS             552..1091
                     /locus_tag="PKH10_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT26995.1"
                     /translation="MGAYRTILGLILLAAPVVTTAQQVIESVAAKIDDTTVITYSDIL
                     QEAALLNLENGAPLHTPLSRELKGKILDSLILRAILFFESRDRDLPLDDRTVQEMVAT
                     YEKLPYLGDLFKSFGISPLEFRMLVKKRLIVRLMMNDLLKRRFPPTAKPTEEEKNKAL
                     DEWFDSLRKKHRIITYSLP"
     gene            1101..1871
                     /locus_tag="PKH10_02345"
     CDS             1101..1871
                     /locus_tag="PKH10_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNT26996.1"
                     /translation="MTRIVTIVNQKGGVGKTTTAVNLAACLGALEKRVLLVDMDPQGN
                     ATSMSALTTPVGRTVYDMMGDPSLTAVYDTELPYLKVMPANRDLTGAEIELLDLEKRE
                     FVLRGILDRVKRKFDLIIIDSPPSLNILTVNNLAAADGLIVPIQCEYFALEGVSRILE
                     TIERIRPLNPHLAIDGIILTMYDKRVKLTFDVEQEVRRHFKKTVFETVIARNVKLSES
                     PSFGKPIILYDIASTGARQYLALAEEYLKRIGKGGTGG"
     gene            1864..2706
                     /locus_tag="PKH10_02350"
     CDS             1864..2706
                     /locus_tag="PKH10_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNT26997.1"
                     /translation="MADRKDKGSFGKLGRGLDELLPTSAGKPILTLRIDQILPSAKQP
                     RAYFDDEKLKELALSIKEHGLIQPIVVKKEGARYRIIAGERRYRAAKIAGIKELPAVL
                     YQGEKDYLIALTENLQRENLNPLEVAEAYGELMKRNGYTQQQLADQIGKSRPEISNYL
                     RLLSLSDRVKKLLATGAVSVGQVRPLTVLPAGEQDPLVERIVAEELSARQVEALTRRR
                     PATAPAKEEKERDDAFAPLETELFRKLQIPVRIKRRMRKIVVMFEFIKMKDFQNFINE
                     LKKK"
     gene            2687..3361
                     /locus_tag="PKH10_02355"
     CDS             2687..3361
                     /locus_tag="PKH10_02355"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT26998.1"
                     /translation="MNLRKNNFLFPLILLLLFPSLVFSFTVVIDAGHGGDEKGAVQGS
                     LIEKDLSLRLARKIQTRLAKGPGIRAVMTREEDRTLPIEERARAIAEAKADLFLSVHF
                     NIDVFMLAETRGLEIYYPADDLAKAPEEALSLYHRNNRSFAFGKMMRDRYLKSELFTV
                     WRHDLNMFAQRDLKIFRLTTTPGLLLEIGYLTSPDDRAHIENEQFLDDMAAFIAETVA
                     EQAGKK"
     gene            complement(3385..4365)
                     /gene="trpS"
                     /locus_tag="PKH10_02360"
     CDS             complement(3385..4365)
                     /gene="trpS"
                     /locus_tag="PKH10_02360"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018449646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNT26999.1"
                     /translation="MRSLSGIKPTGTLHIGNYFGAAKQFEELQKKGYDCFYFVADYHT
                     LNTTPDPGELTENSWGVVLDYLAFGLDPEKSTLFLQSQVPEVVELAFILGNVTPMGLM
                     QRAHSYKEKSDKGELINVGLFYYPLLMAADILLYDSDVVPVGQDQKQHVEMARDIAQK
                     FNQQYNSEVFKLPEPLIREDVAVVPGTDGRKMSKSYGNTIEMFASEKELKKQVMGIVT
                     DSTPLEQPKDPDTCNVFALYKLFATPQEQALMKDKYRAGNYGYGTAKKELLEKLMQFF
                     KPMREKREELDRNRYYVERVLREGAQRARTAAVEKIKKVKKVAGLVGNIY"
     gene            complement(4480..6156)
                     /locus_tag="PKH10_02365"
     CDS             complement(4480..6156)
                     /locus_tag="PKH10_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27000.1"
                     /translation="MKKTIIGGVLTAPLLVSFLIIGLAVLSACKENPQAPKDDALMDF
                     EIVPDSDQTEFPDHKETDSVDLDITEEATTDTDGNIWEDVDCVDAPQPWMAEGDPYDD
                     RDILYQYYGDFDVIVQDPENVRGLWNDKFVGYDTGSIKECTCADWVSSGACAEQFPFE
                     PIIVEERNKPDTPTYERKPLLGIFECDMLLTPPRQWELAWRYGADPKYNAENGKVLFP
                     MHSYNAWTFPYAVNAHLFDIASQQLITISPEYSNAYFNGRYVFMTLRDAAKNYEEPDK
                     NYYEPVPWSKLVYYDTVEHKYGYAWYGPDFYYGGSIHASDTYIIMSIQQLPDVSGGRV
                     VYTKIGDWQNWKELKFMTTSSQGFIDAGFPSMVGQYVAFYNWDAEVAFCDLEKGDPGC
                     FKVSRDDENGRMPKILDSKAVYYSAEDKTTKIFSLVKANITDPQAIKYTTIVSHDKVL
                     DIDDIDKDFLLYGKEIDKRSNGDPLMINCFYRFSDGKSFCMDDADHDAKIPTDYSYLP
                     GGHYLVYQSWYDLVLRDMECYCDQFPARCPYADYTPKKAKVLLQGSNERR"
     gene            complement(6353..>9360)
                     /locus_tag="PKH10_02370"
     CDS             complement(6353..>9360)
                     /locus_tag="PKH10_02370"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HNT27001.1"
                     /translation="DPTGTGDIETTDEGEFITAGNASNSNNYGEDATAGGADDEGAPS
                     NEDDKGDDAAREIVEADIYKVEGNILWVLNQYRGLVAIDVTEPTALKILGRSSFKGFP
                     FEMYVQEGRAYVMVTGLRYNPSEKSEDGGYYDASRTFSELIVVDIADPAKQKILGRYM
                     MEGTIIDSRQVGDVLYVAASETRYYWYYCDNAEERGKDQITLLSLNIADPKDIKKADE
                     EHLDGTGYALYVTDHSIYVAETNLYYWQDGYYTDGVPVHYFDISDPAGAIEKKGVIKT
                     RGYIGDRWKMHEKDGAFFAVTSTSQWWNGENAVESFDVTDPSNPKKISEFTFMTNQEL
                     HGARFDGNRLYAVTYFQQDPLHVIDITDPAKMKELGQLAVPGWSTHIEIRGTKLLTVG
                     IDDQDGWNAKVSMYDVADPTNPTEMTTVELGGAENGYSWSEATNDWKAFKIYDAIGLI
                     LVPTSEWNYNTYREVNKLHLIDFDLETGLTKRGSVESPGYVRRGVVVGDHIASVGDTT
                     VMMIDYSDRDNPEILSSLPVAYAVENLFSCAGKLCGTKGTSWYDSDIRLVTYDASKDY
                     PVNWSSDRVRGQYVYAMQNDAKAVHMFSYDSSYTYDEEKGETYQENVYATRFDLAEGK
                     DPKPAGSLKLELPPQDQEGEYWYWYGRAFAVAESKAAGMMSTRYDYYCLADGAMVDCA
                     TEGGSYDNYYYYNNSKLAVYDLRDLSKESAAPVLFDKEFRGVTYNNTVIARGDKLWAT
                     DCKLHAWDDEGRELLICYAQAVDVSDPAKPKLAEKYNIPGELVGMSDDGTIFYTLMRT
                     WIKTDEDGKRGDYYYSSNYLYHLNILKKSADGKKLTLVKVIPYHEEYTYTENTSEYLW
                     SSYLIQDKKVFLNTIQQTYHWEEQQCSYYSTTVERTSTVAAYDGATGAKIMEDVVPDG
                     IGMSGVDGGGVLIQRQTDAFYYYYYGSEGREYTYIAPDGTMTELALPEVSYYADYYYY
                     YNGNKAVRQGDNLVIARGWEGLTTVSLEP"
BASE COUNT         1907 a         2783 c         2623 g         2047 t
ORIGIN      
        1 ccaccgggac ctgcgccgcc catgatagag atacgcaccg ccaccttcct gaaaagcgcc
       61 gcccatcccg ccgacctgcc gcccccgacg cttcccgaat acgctttcat aggccgttcc
      121 aatgtcggca agtccagtct tatgaacctt ctgctcggtc gtcggaacct cgtcaagacg
      181 agcaagaccc ccggtaagac ggtgacaatg aacttcttcg acatcaacgg ggcgttccgg
      241 ttcgtcgatc ttcccggtta cgggttcgcc aaacgatcga aaagcgaaca agaggtctgg
      301 cgacggacca tagagtccta cctcacccgg cgcgataatc tgcggctcat cctcctgctc
      361 atcgacgggc gtcacggtcc ccagcctaac gacgagcaga tgatcgcgtt cctcgaccac
      421 caccggctcc cgtggctacc ggtattcacc aagatagaca aactctcgac cgctgagaag
      481 cagcgcctgc gccgcggcaa ccccggcgca ccgtgcgttt cggcgaccac cggcgagggg
      541 aaggaagaga catgggcgca tatcgaacga tactggggct gatactgctc gcggcgccgg
      601 tcgtcacgac ggcccagcag gtcatagaga gcgtcgcggc gaagatagac gatacgaccg
      661 tcatcaccta ctccgatatc ctgcaggagg cggcgctcct gaacctcgag aacggcgccc
      721 cactgcatac accgctctcg cgcgaactca aagggaagat ccttgactct ctcatcctcc
      781 gggctatcct gttctttgag tcacgggacc gtgatcttcc ccttgacgat cggacggtac
      841 aggagatggt tgccacctac gagaagcttc cctacctggg cgatctgttc aagagtttcg
      901 gcatctctcc gctcgagttc cgtatgctcg tcaagaagcg gctcatcgtg cgactgatga
      961 tgaacgacct cctgaaaagg cgttttccgc cgaccgcgaa accgaccgag gaggagaaga
     1021 acaaggcact cgacgagtgg ttcgactccc tgcgcaaaaa acaccgcatc ataacctact
     1081 ccctacccta gagggacccc atgacacgta tcgtcaccat cgtcaatcag aaaggcggcg
     1141 tcggcaagac gacgaccgcc gtcaatctcg ccgcctgcct cggcgcgctc gaaaagcgcg
     1201 ttctgctcgt cgacatggac ccgcagggca acgcgacctc aatgagcgcc ctgaccacgc
     1261 cggtcggtcg caccgtctac gatatgatgg gcgacccgtc gcttaccgcc gtctacgaca
     1321 ccgaactccc ctatctcaag gtgatgccgg ccaaccgtga cctcacgggc gccgagatag
     1381 aactgctgga cctcgagaag cgagaattcg tactgcgcgg catacttgat cgcgtcaaac
     1441 ggaagttcga cctcatcatc atcgactcac cgccctcgct caacatcctc accgtcaaca
     1501 acctcgcggc ggccgacggg ctcatcgttc cgatacagtg cgaatacttc gcgctcgaag
     1561 gggtgtcgcg gatactcgag accatcgagc gtatccgccc tctgaacccg catctggcca
     1621 tagacggcat catcctcacg atgtacgaca agcgggtgaa actcaccttc gatgtcgaac
     1681 aggaggtgcg gcggcatttc aagaagaccg tgttcgagac ggtcatcgcg cgcaatgtca
     1741 aactatcgga atcgcccagt ttcggcaaac ccatcatact ctacgacatc gcctcgaccg
     1801 gcgcgcggca gtacctcgcg ctcgccgagg aataccttaa acgcatcgga aagggaggga
     1861 caggtggctg acaggaagga caagggatcg ttcggcaaac tgggccgcgg actggatgag
     1921 ttgctgccga cctctgcggg caagccgata ctcacgctcc ggatcgatca gatcctgccg
     1981 agcgcaaaac agccgcgcgc ctatttcgac gatgaaaaac tgaaggaact cgcgctctcg
     2041 ataaaggagc acgggctcat ccagcccatc gtggtcaaaa aagagggggc gcgataccga
     2101 atcatcgccg gcgagcggcg gtaccgcgcc gcgaagatcg ccgggatcaa ggaactgccg
     2161 gcggtcctgt atcagggcga aaaggactat ctcatcgcac tgaccgagaa cctgcaaagg
     2221 gagaacctga acccgctcga ggtcgccgag gcgtacggcg aactgatgaa gcggaacggc
     2281 tatacccaac agcaactcgc cgatcagatc ggcaagtcgc gccccgagat atcgaactat
     2341 ctgcgactct tgtcgctttc cgaccgggtc aagaagcttc tcgcgaccgg cgcggtgtcg
     2401 gtgggacagg tgcgcccgct cacggtgctt ccggccggcg aacaggatcc gctcgtcgaa
     2461 cgcatcgtcg ccgaagaact ctcggcacgg caggtcgagg cgctgacccg tcggagaccg
     2521 gcgaccgccc cggcgaaaga ggagaaagag cgggacgatg cctttgcccc gctcgagacc
     2581 gaacttttcc ggaaactgca gataccggtg cgcatcaagc ggcggatgcg gaagatcgtc
     2641 gtgatgttcg agttcatcaa aatgaaggac tttcagaatt tcatcaatga acttaagaaa
     2701 aaataatttt cttttcccgt taattcttct gcttcttttt ccctctctcg tcttctcctt
     2761 caccgtcgtc atcgacgcgg gacacggcgg cgacgagaag ggcgcggtgc agggttcgct
     2821 catcgagaag gacctttccc tgcgactcgc ccgcaagata cagacccggc ttgccaaggg
     2881 gccgggcatc cgcgccgtca tgacccgcga ggaggaccgc acgctcccga tcgaagagcg
     2941 ggcccgcgcg atcgccgagg cgaaggccga cctgttcctc tccgtccact tcaacatcga
     3001 tgtcttcatg ctcgccgaga cgcgcggtct ggaaatatac tatcccgccg acgacctcgc
     3061 gaaggcaccc gaggaagccc tttcgctcta ccaccggaac aaccgctcct tcgcgttcgg
     3121 taagatgatg cgcgaccgct acctcaagtc ggaactcttc accgtctgga gacacgacct
     3181 caacatgttc gcacagcgcg atctcaagat attccggctg accacgacgc ccggacttct
     3241 gctcgagatc ggctacctga cctcccccga cgaccgcgcc cacatcgaga acgagcagtt
     3301 cctcgacgac atggccgcct tcatcgcaga gacggtggcc gaacaggcgg gaaagaaata
     3361 ggccggaaat agcccctcac ctgttcagta tatattgccg acgagcccgg cgaccttctt
     3421 gaccttcttt atcttctcga ccgccgcggt ccgcgcccgc tgcgcccctt cacggagcac
     3481 ccgctcgaca tagtagcggt tcctgtcgag ttcctcacgc ttctcgcgca tcggcttgaa
     3541 gaactgcatg agtttttcca acagttcctt cttcgccgta ccgtagccgt agttgccggc
     3601 gcggtacttg tccttcatca acgcctgttc ctgcggcgtc gcgaagagtt tgtagagggc
     3661 gaagacattg catgtgtcgg gatctttcgg ctgttcgagc ggcgtcgaat cggtgacgat
     3721 ccccatcacc tgctttttga gttccttttc cgacgcgaac atctctatgg tgttgccgta
     3781 acttttcgac atcttgcggc cgtcggtccc cggcaccacc gcgacatcct cgcggatgag
     3841 cggttcaggc agtttgaaga cctcgctgtt gtactgctgg ttgaatttct gggcgatgtc
     3901 gcgcgccatc tcgacatgct gtttctggtc ctgccccacc ggcaccacat ccgaatcgta
     3961 cagcaggatg tcggccgcca tcaacagcgg atagtagaac agtcccacat tgatgagttc
     4021 ccctttgtcg ctcttttcct tgtaactgtg ggcccgctgc atgagcccca tcggcgtcac
     4081 attgccgagt atgaaggcga gttcgaccac ctcggggacc tgcgactgaa ggaacagcgt
     4141 gcttttctca gggtcgagtc cgaaggcgag atagtcgagc acgacgcccc acgagttctc
     4201 ggtcagttcg cccgggtcgg gcgtggtgtt cagcgtgtgg tagtcggcga cgaaatagaa
     4261 gcagtcgtag cccttcttct gtagttcctc gaactgtttg gcggcgccga aatagttgcc
     4321 gatgtgcagg gtgccggtcg gcttgatacc cgaaagagaa cgcatcgtga cctccgaagt
     4381 gtttcagcgc gttcatcatt gcaaagcggg gccggttagt caagaaaaga acgggggaga
     4441 gaaaacgagt gtttctccag atcttttacg ccacaaattc tagcgtcttt cgttgctacc
     4501 ctgcagcaag actttcgctt tctttggcgt gtaatcggcg tagggacagc gggcggggaa
     4561 ttggtcgcag tagcactcca tgtcgcgcag gaccagatcg taccacgact gatagacgag
     4621 ataatgacca cccggcagat aggaatagtc ggtcggtatc ttcgcgtcat ggtcggcgtc
     4681 gtccatgcag aatgatttgc cgtcgctaaa gcgatagaaa cagtttatca taagggggtc
     4741 gccgtttgat cgcttgtcta tctccttccc gtaaagaaga aaatctttgt ctatgtcatc
     4801 tatatctaac actttgtcgt gcgaaacaat agtcgtgtac ttgatcgcct gcgggtcggt
     4861 aatgttggct ttgacgagag aaaagatttt cgtggttttg tcttcggcag agtaataaac
     4921 cgctttgcta tcaagaatct tcggcatccg tccattctcg tcatcccgcg ataccttgaa
     4981 acatccgggg tcccccttct cgagatcgca gaaggcaacc tcggcatccc agttgtagaa
     5041 ggcgacatat tgccccacca tcgagggaaa acccgcgtca atgaatccct gtgacgaggt
     5101 ggtcatgaac ttgagttctt tccagttctg ccagtcgcct atcttggtgt agacgacccg
     5161 cccccccgaa acatctggca attgctggat actcatgatg atatatgtgt cgctcgcgtg
     5221 gatcgatccg ccgtagtaaa agtccggtcc ataccacgca tagccgtact tatgctcgac
     5281 ggtatcgtaa tagaccaact tcgaccacgg cacgggttca taatagttct tgtcaggttc
     5341 ctcataattt tttgcggcat cacgaagtgt catgaaaaca tagcgtccat taaagtaagc
     5401 gttggagtac tcgggagaaa tggtgatcaa ttgttgagaa gcaatgtcaa agaggtgagc
     5461 atttacggca taaggaaagg tccatgcgtt ataagagtgc atagggaaaa gcactttacc
     5521 gttttctgcg ttatatttag gatctgcgcc gtaacgccaa gcaagttccc actgccgggg
     5581 aggggtcaaa agcatgtcgc attcaaatat tcccagaaga ggctttcttt cataggtcgg
     5641 cgtgtccggc ttattcctct cctcgactat tatcggctcg aatggaaact gttcagcaca
     5701 cgctccgctg gacacccaat cagcgcaggt gcactctttt atagaaccag tatcataacc
     5761 gacaaacttg tcattccaca gtccgcggac attctccgga tcctgtacaa tcacatcgaa
     5821 atcgccatag tattgatata aaatatcacg gtcatcgtat gggtcgccct cggccatcca
     5881 tggctgtggc gcatcgacac aatcgacatc ttcccatatg ttgccatcgg tatcggtcgt
     5941 agcctcttcc gtaatatcca aatcaacaga gtccgtttcc ttgtgatccg ggaactctgt
     6001 ttgatcggaa tcgggaacga tttcaaaatc catcagggca tcatccttgg gtgcttgggg
     6061 gttttctttg cacgcgctca acaccgccaa ccctattatg agaaaagaaa cgagtagggg
     6121 ggcggtcaac acgcccccga taattgtttt cttcattctt tagcacccac tccagtaaca
     6181 gtctcccgta tcataaccgg tacaggtcga attgcaatag gccgtgccac tgttccactg
     6241 gttaagagaa tcgggtcatg gtcatttctt ggctatgacg agaaaggtac ggaaataaaa
     6301 agaacggggg gcccgcaggc cccccgtgat gtttcgggaa ctcccgtgac gcttacggtt
     6361 ccagtgaaac ggtggtgagc ccttcccagc cacgcgcgat gacgaggttg tcgccctgcc
     6421 ggaccgcctt gttgccgttg tagtaatagt agtaatcggc gtagtaggag acctcgggca
     6481 acgcgagttc ggtcatcgtg ccgtcggggg cgatgtaggt gtattcgcgc ccttcgctac
     6541 cgtagtagta atagtagaag gcgtcggtct ggcgctggat aagcacgcca ccgccgtcaa
     6601 cgccgctcat gccgatgccg tcggggacca catcttccat gatcttcgcg ccggtcgcgc
     6661 cgtcataggc cgccacggtc gaggtgcgct cgacggtggt cgagtagtag gagcactgct
     6721 gctcttccca gtggtaggtc tgctgaatgg tgttcaggaa caccttcttg tcctgaatga
     6781 ggtaactgct ccagaggtat tcggaggtgt tctcggtgta ggtgtactcc tcgtggtacg
     6841 ggatgacctt gacgagcgtc agtttcttgc cgtcggcgct cttcttgagg atgttcagat
     6901 ggtacaggta gttgctcgag tagtagtagt cgccgcgttt gccgtcctcg tcggtcttta
     6961 tccaggtgcg catcagggtg tagaagatgg tcccgtcgtc gctcatgccg accagttcgc
     7021 ccgggatatt gtatttttcg gcgagtttcg gcttggccgg atcggacaca tcgaccgcct
     7081 gcgcgtagca gatgagcagt tcgcgccctt cgtcgtccca cgcgtgcagt ttgcagtcgg
     7141 tggcccacag tttgtcgccg cgtgcgatca cggtgttgtt gtaggtgacg ccgcggaact
     7201 ctttgtcgaa cagcaccggc gcggccgact ctttggaaag atcgcgcagg tcgtacaccg
     7261 ccagtttcga gttattgtag taatagtagt tgtcgtagga accgccctcc gtcgcgcaat
     7321 cgaccatggc gccgtcggca aggcaataat agtcgtagcg ggtgctcatc atgccggccg
     7381 ccttgctctc ggccaccgcg aaggcgcgac cgtaccagta ccagtattcg ccctcttgat
     7441 cctgcggagg cagttcgagt ttgagcgagc cggccggttt cgggtcctta ccctcggcga
     7501 ggtcgaagcg ggtcgcgtac acattctctt ggtaggtctc gcctttctcc tcatcatagg
     7561 tgtaggaact gtcgtacgag aacatgtgga ccgccttcgc gtcgttctgc atcgcgtaga
     7621 catactggcc gcggacacgg tcgctcgacc agttcaccgg gtagtccttc gacgcatcgt
     7681 aggtcacgag ccggatgtcg ctgtcatacc acgaggtccc cttcgtgccg cacaatttcc
     7741 cggcgcacga gaagaggttc tcgacggcgt aggcgaccgg cagactcgac agtatctcgg
     7801 ggttgtcgcg gtcgctatag tctatcatca tgacggtggt gtcgccgacc gaggcgatat
     7861 ggtcgcccac caccacgccg cgccgcacat agccggggct ctcgaccgag ccgcgcttcg
     7921 taagaccggt ctcgagatcg aagtcgatga ggtgcagttt attgacctcg cggtaggtgt
     7981 tgtagttcca ctcgctcgtc ggaacgagga tgagcccgat ggcgtcgtag atcttgaagg
     8041 ccttccagtc gttggtcgcc tcgctccacg agtagccgtt ctcagcgccg cccaactcga
     8101 cggtggtcat ctcggtcggg ttcgtcgggt cggccacatc gtacatggag accttcgcgt
     8161 tccagccgtc ctgatcgtcg atgccgacgg tgaggagttt cgtgccgcgt atctcgatat
     8221 gggtggacca gccggggacc gcgagttggc ctagttcctt catcttggcc ggatcggtga
     8281 tgtctatcac atgaagggga tcctgctgga aataggtcac ggcgtagagc cggttcccgt
     8341 cgaaccgcgc gccgtgcagt tcctgatttg tcatgaaggt gaactcgctt atcttcttcg
     8401 ggttcgacgg atcggtcaca tcgaacgact cgaccgcgtt ctcaccgttc caccactggc
     8461 tcgtggaggt caccgcgaag aaagcgccgt ccttctcgtg catcttccag cggtcgccga
     8521 tgtagccgcg ggtcttgatg acccccttct tctcgatggc gccggccgga tcgctgatgt
     8581 cgaagtaatg caccggcacg ccgtcggtgt agtagccgtc ctgccagtag tacaggttgg
     8641 tctcggcgac atagatgctg tggtcggtga catagagcgc gtatccggtg ccgtcgagat
     8701 gttcctcgtc ggcctttttg atgtccttcg gatcggcgat gttgagcgac aggagcgtta
     8761 tctgatcctt gccgcgctct tccgcgttat cgcagtagta ccagtagtag cgggtctcgc
     8821 tcgccgcgac gtagagcaca tcgcccacct gccgcgagtc gatgatggtc ccctccatca
     8881 tgtaacggcc gagtatcttc tgcttggccg gatcggcgat atcgacgacg atgagttccg
     8941 agaaggtgcg cgacgcatcg tagtagccgc catcctcgct tttctccgac gggttgtagc
     9001 gcagaccggt caccatgaca taggcgcgcc cctcctgcac atacatctcg aacgggaagc
     9061 ccttgaacga cgagcggccg agtatcttga gcgccgtcgg ttcggtcaca tcgattgcga
     9121 caaggccgcg atactggttg agcacccaga ggatgttccc ctcgaccttg tagatgtcgg
     9181 cctcgacgat ctcgcgggcc gcgtcatcgc ccttgtcgtc ctcgttgctc ggcgcccctt
     9241 cgtcgtcggc gcccccggcg gtggcgtcct ccccgtagtt gttgctgttc gaggcgttgc
     9301 cggcggtgat gaactcaccc tcgtcggtcg tctcgatgtc gccggtgccg gtcgggtcgt
//
LOCUS       DAOQUE010000044         9281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8299_length_9281_cov_4.172556, whole genome shotgun sequence.
ACCESSION   DAOQUE010000044 DAOQUE010000000
VERSION     DAOQUE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8299_length_9281_cov_4.172556"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PKH10_02375"
     CDS             complement(<1..1073)
                     /locus_tag="PKH10_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNT27002.1"
                     /translation="MKRGVGGRYEMQNIHGEMVKAFVPIPLPPVPPVDLSGNRQRALE
                     RALVALGRLDSISTLLPDPLLFLYAYVRREALLSAQIEGTQSSLSDLLLFELEETPGV
                     PVDDVREVSNYVAALDHGVARLKADFPLSNRLVREMHALLLHKGRGSEKTPGEFRHSQ
                     NWIGGTRPGNAHFVPSPAASIEDCMAAWERFLHDGTQPALIKAALAHVQFETIHPFLD
                     GNGRLGRLFIALILHHEHLLSQPLLYLSLYFKRQRSEYYRLLDVVRLEGDWEAWIDFF
                     LEGVAQTAGEAVSTAQRLFALHREDTERLQSMKGSSSALKVYTTMKERPMTTLSEASK
                     RSGLSFPAVAKGMNALMRAGIVR"
     gene            1278..2651
                     /locus_tag="PKH10_02380"
     CDS             1278..2651
                     /locus_tag="PKH10_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27003.1"
                     /translation="MRPLSALLPLAAALLLAACSNSIARPADSDAAAPAADDTISDGS
                     GGGDGERDEIAADAAAGDDLPVVDEEADAVPDADPFVTLERTTVQWGTPADDYLVDLA
                     ADGSGGYYVAGSSGGALPGCANAGAYDLFLIRFAADGTRAWSRELGTALDDHGYSIVR
                     GKEGLVSVGGVTFGVFDGAAGAGGLDLFIATWQADGTPAGLQQWGTAVDDRLSGMAGD
                     GSGGFILLSGDALSQRRADGSEVWSKPELLPFNHFYDLTTAPDGTLLLAGVEQYPATR
                     DRNNHCAVTAVDTEGALLWSRTTYSPEYCTAVALSPAGDIYAIGYADQYDGAPVEGDI
                     LLVKFNAAAEQQWTVPWGTEANDEGRNILTDQKGDIYLLGTTERIPPEHPAAVGRDIL
                     LLKFSQKGTPLWMAQWGSDQDDGPGGMFIDPQGRLVVAGYTAGSIDGNPSAGGVDMFI
                     SAITLPE"
     gene            2854..3060
                     /locus_tag="PKH10_02385"
     CDS             2854..3060
                     /locus_tag="PKH10_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNT27004.1"
                     /translation="MFPRNVGAFDRTIRILLGLDILIAGWYYEEWWGLLGLLPVLTAL
                     VGSCGLYTLFGVNTCRVKVAEEKK"
     gene            complement(3068..4162)
                     /locus_tag="PKH10_02390"
     CDS             complement(3068..4162)
                     /locus_tag="PKH10_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNT27005.1"
                     /translation="MTFSLTALFTAGLATFFTPCVLPLIPVYLAAVAGMERPGDRPSG
                     ARLFLRAAAFSLGIVIVFALFGAAAAALGGLIKTYNHYLLLFGGVLVLLFGLKFLGII
                     QIPLFDRIARLDESRFSSRLGLVNAFAMGLIFGAGWSPCVGPILGAALTFTATSGASA
                     ATGMFYLALYGLGFTAPFLATALFADRLLPILRRANRGLPLFEKLVGVALIAGSLYLF
                     GEAVNPPPPAAAAIAAPQAKLERFPLPDGLPALVEFYSEECPICKKMEPVVKRIFERC
                     DGNKVTLRKVDISKPENRSYAAAHRILGVPTFILFDVDRTEVARLVGLQTEDSIYQAI
                     ATLRGEECDGVGLLPRSEPVPVTGSACGTR"
     gene            4321..5457
                     /locus_tag="PKH10_02395"
     CDS             4321..5457
                     /locus_tag="PKH10_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT27006.1"
                     /translation="MKNVFFFALFLFAFYGVYTLLHWFFWRGLVAAFPLPLWARRGVI
                     VFFALMFLFPIANHASSALAGTLPCRGVAWIGFLWLGFLFYYLFVGGAFELAAFAVKR
                     FAEEELSRRALFIAASALSVMVIAYGFHEASQIRVRHHTLTTAKLPEFIAKIRIVQVS
                     DVHFSQLHGVAFADRLTALIAEQRPDLLVMTGDFLDRGLVDEEGIAAKWQALEAPLGK
                     YAVTGNHEFYNGIDHSVALIEKSGFTLLRNRGETRANNAFVIAGVDDPAAARFGEEPP
                     TAKRVLESLPSKPYRIFLRHQPKDDPETAGLFDLQFSGHTHNGQIWPFSLFVSIPFPM
                     RGGIYQQKEGGTTVVSSGAGTWGPPIRVLAPPDIVVVDLVRKVE"
     gene            5479..6849
                     /gene="lpdA"
                     /locus_tag="PKH10_02400"
     CDS             5479..6849
                     /gene="lpdA"
                     /locus_tag="PKH10_02400"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNT27007.1"
                     /translation="MEHLKLLIIGAGPGGYVAAIRAAQLGLSPVVVERSNDLGGTCLN
                     WGCIPAKALIRAAEAFEDAQEACRFGVHAEPSFVWSEVVAHAKGAVAKNNKGIEFLFK
                     KHNIRLIRGAARFTGPKEIAVGDDRYTADHIIIAAGSIVKDLPHITVDGDGVWSSDHA
                     LFCEKLPKSVAIIGAGAIGVEFAYIFSVFGVETHLIEALDRFVPLEDGEVSKELYKEF
                     RKRKIKCYPGTCVDKAEQGPEGMTLTLAGGKSVTVEKILSAVGRRPGSGSLGLDAAGI
                     ATDPRGFIPVNEHYQTTVPGVYAIGDLIATPMLAHTAEHEGSHAAEHIAGLHPHPIDY
                     RQNPGCTFCEPAIASVGLTEERAKELGVAYAVGKFPFSANGKAVASGATAGFVKVLIA
                     ADSHELLGAHIIGRGATDLIAEFLPALRLKAKAEDIVEAIHPHPTLCEASLEAVLDAL
                     GRVIHK"
     gene            6866..7456
                     /locus_tag="PKH10_02405"
     CDS             6866..7456
                     /locus_tag="PKH10_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNT27008.1"
                     /translation="MDAVTVIDFETTGLEFAKEDRIVEIGAVKWRDHRITGIYDALVN
                     PGRPIPAEVVRVHGITDSMVASAAPLDVAVPELLDLIGDDLVVMHNVEFDSGFLKAQM
                     KLLGIERELTYYDTLPAARKRFLGSPNYRLATLKNFLNLKQLGTMHRALADAYVTLQL
                     YLKLENLWAIDSRSQLEKQLRELTQRRAPGARVVTM"
     gene            complement(7466..8932)
                     /gene="glgA"
                     /locus_tag="PKH10_02410"
     CDS             complement(7466..8932)
                     /gene="glgA"
                     /locus_tag="PKH10_02410"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014936337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNT27009.1"
                     /translation="MKIAMISSECVPYAKTGGLADVVGALPQALAGLGHEVVVILPLY
                     GCIDQEKHRLSPCMEQLGVWMGNTLEWCAVHQATGDGKVPVYFIEHNKYFQRDGLYHD
                     AWFNDYPDNPRRFAFFSRAALQLLRDLYWMPDIVHAHDWQAATAVAYLKVWHWNDPLL
                     AKAAGILTIHNLAYQGVYGTEHYDYSGLRWEHFKPDIFEDHGRVNYLKGGIHFADRVN
                     TVSPTYAKETLSGPLSCGLAPFLSAKGDRYLGILNGVDYARWSPGNDRYLPLPYSADD
                     LAGKAAAKELLQRTLALAVDPTVPVIGIVSRFVAQKGLDVVASALDRILADMVVQFAV
                     LGAGDKVLEQYYGALAARYPGRVGAYIGYSDELSHIIEGGSDLFLMPSQYEPCGLNQI
                     YSLRYGTLPIVRATGGLEDTVEQYDEKTGAGTGFKFQYLSPEAVYDTVGWAVSTWYDR
                     PAHFAAMRREAMAKDFSWEESAKRYVELYRRALEDKKP"
     gene            9091..>9281
                     /locus_tag="PKH10_02415"
     CDS             9091..>9281
                     /locus_tag="PKH10_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNT27010.1"
                     /translation="MWFYEQYDEKASLGLKVRRKLHEEQSKFQKIAVFETEFFGNLLT
                     LDGLVMLCEKDEFVYHEMIT"
BASE COUNT         1679 a         2905 c         3008 g         1689 t
ORIGIN      
        1 tcgcgcacta tccccgcccg cataagagcg ttcatcccct tggcgacggc cgggaatgaa
       61 agaccgcttc gcttgctggc ctccgacaga gtggtcatcg gtcgttcctt catggttgta
      121 tagaccttga gcgccgaaga agatcccttc atcgactgaa gccgttcggt atcttcgcgg
      181 tgcaaagcaa aaagccgttg cgccgtcgag accgcttctc cggcggtctg tgcgacgcct
      241 tccagaaaga agtcgatcca ggcctcccaa tcgccctcga gccgtaccac atcgagaagt
      301 cggtaatatt cgcttcgctg gcgcttgaaa taaagactca gatacaagag cggctgagag
      361 agcagatgtt catgatgcag aataagcgcg atgaacagcc gtcccaaccg gccattgcca
      421 tcaaggaagg gatggatggt ctcgaactgg acatgggcga gcgccgcttt tatcagggcc
      481 ggttgcgtcc cgtcatgcag gaaccgctcc catgccgcca tgcaatcttc tatcgatgcg
      541 gccggagacg gcacgaagtg ggcattgccc ggccgtgttc cgccgatcca attctgggaa
      601 tgtcggaact ctcccggcgt tttttcgctg ccgcgtcctt tgtgcagcag caaggcatgc
      661 atctcgcgga ccagccggtt cgaaagaggg aagtctgctt tcaagcgggc cacgccgtgg
      721 tcaagcgccg ccacataatt ggaaacctcc cgcacatcgt cgacgggaac gccgggcgtt
      781 tcttccaatt cgaaaagcag gaggtcggaa agcgatgact gtgttccctc tatctgggcc
      841 gacaataatg cttcacggcg tacatatgcg tagagaaaca gtagcggatc gggtaacagc
      901 gttgaaatgc tgtccagccg tcccaacgcc accagtgccc gctcgagcgc tcgttgtcgg
      961 tttccgctaa gatccactgg cggtacgggc ggcaacggga tcggtacaaa agccttaacc
     1021 atctcgccgt gaatgttctg catctcatat cttccgccaa ctccccgttt catttgattg
     1081 ttctcctatc gtttaataac gaccgctgct attaagcaaa ataagcattt cgtttaataa
     1141 ggcaagtttt cttttcatac tatttaataa ggaacctaag accgtccgcc cgaaagcgcg
     1201 acgatcttcc gccgcaaaac tttacttcgc cgccactggt ctgttactgt gcctttcaac
     1261 cgcgaccgag gtgcccgatg agaccgctct ccgcactgtt gccgcttgcc gccgcgcttc
     1321 ttctcgccgc ctgctccaac agcatcgccc gccccgccga cagcgacgcg gcggcccccg
     1381 ccgccgacga caccatctcc gacggctccg gcggcggcga tggggaaagg gacgagatag
     1441 cggcggatgc tgccgccggg gacgatctgc cggtcgtcga cgaggaggcc gatgcggtgc
     1501 ccgacgccga tcctttcgtc acgctcgaac ggacgacggt gcagtggggc acgcccgccg
     1561 acgactatct ggtcgacctt gccgccgacg ggagcggcgg ctactatgtc gccgggtcga
     1621 gcggcggcgc gttgccggga tgcgcgaacg ccggggcgta tgatcttttt ctcatccggt
     1681 tcgccgcgga cggcacgcgt gcgtggagtc gggaactcgg caccgcgctg gacgatcacg
     1741 gctatagtat cgtgaggggg aaggagggac tggtctcggt gggcggcgtc accttcggcg
     1801 tgttcgacgg cgccgccggc gcgggaggtc ttgacctttt catcgcgacg tggcaggccg
     1861 acggtacgcc ggcagggttg cagcagtggg gcacggcggt cgacgaccgg ctctccggca
     1921 tggccggcga cggctcgggc ggtttcatcc tgctttccgg agatgcgctt tcccaaagac
     1981 gcgccgacgg ctccgaggtg tggagcaagc ccgaactgct gcccttcaac catttctacg
     2041 atcttacgac cgcccccgac ggcaccctcc tgctggcggg ggtcgaacaa tatcccgcca
     2101 cgcgcgaccg caataaccat tgcgccgtga ccgcggtcga tacggagggc gccctgttgt
     2161 ggagccggac gacctacagc cccgagtact gcacggcggt ggccctctcc cccgcgggcg
     2221 atatctacgc gatcgggtat gccgaccagt atgacggagc gccggtcgag ggcgatatcc
     2281 tgctggtcaa gttcaacgcg gcggccgaac agcagtggac ggtcccgtgg ggtaccgagg
     2341 ccaacgacga gggaaggaac atcctgaccg atcagaaagg ggacatctat ctcctcggca
     2401 cgaccgagcg gatcccgccg gagcatcccg ccgcggtggg ccgcgatatc ctgctgctca
     2461 aattctcaca gaaggggacg ccgctctgga tggcgcagtg gggtagcgat caggacgacg
     2521 gtccgggcgg catgttcatc gacccgcagg gacggctcgt tgtcgcgggc tataccgccg
     2581 gttcgatcga cggcaaccct tcggcgggcg gcgtcgacat gttcatttcg gcgatcaccc
     2641 tgccggagtg aacctgtaag agcgttgaac ccgcaacgaa gaccctcgat agtcaatagc
     2701 tgttccccgt tcccgcccgc cccgacccct tctttcccgg gaatattctc cgctttgatt
     2761 tcctttttct gtaaagaatc taatatcttg actctctagc cttcccccgc tatacttttc
     2821 cccgtaccgt tcaaccacac gaaggaggca accatgttcc ccagaaatgt cggcgccttc
     2881 gatcgcacca tccgcatcct gctcgggctc gacatcctca tcgccggctg gtactacgag
     2941 gagtggtggg gcctgctcgg cctcctcccg gtcctcaccg cgctcgtcgg ttcctgcggg
     3001 ctctatacgc tgttcggcgt caacacctgc cgggtgaagg tcgcggaaga gaagaagtag
     3061 gggcggttca ccgcgtcccg caggcgctcc ctgtgacggg gaccggttca ctgcggggga
     3121 ggagcccgac gccgtcgcat tcctcgccgc gcagggtcgc tatcgcctga tagatcgaat
     3181 cctcggtctg gagcccgacc agccgcgcca cctcggtgcg atcgacatcg aacagtatga
     3241 aggtcggcac gccgagtatc cggtgggcgg cggcgtagga tcggttctcc ggcttggata
     3301 tgtccacttt gcggagcgtc accttgttgc cgtcgcaccg ctcgaatatc cgcttgacga
     3361 ccggctccat ctttttgcag atagggcact cctcgctgta gaattcgacg agcgcgggaa
     3421 gcccgtcggg gagcgggaac cgctcgagtt tcgcctgtgg cgcagcgatg gcggcggcgg
     3481 ccggcggcgg cgggttcacc gcctcgccga agagatagag cgacccggcg ataagcgcga
     3541 cgccgaccag tttctcgaac agcggcaacc cgcgattggc gcgccgcagg atgggcagga
     3601 gccggtcggc gaacagcgcg gtcgcaagga acggggcggt gaacccgagc ccgtagaggg
     3661 ccagatagaa catcccggtg gccgccgagg cgcccgaggt cgcggtgaag gtgagcgccg
     3721 cgccgagtat cgggccgacg cagggggacc agccggcgcc gaagatcagc cccatcgcga
     3781 aggcgttcac gaggccgagg cgcgaggaga accgggactc gtcgagccgc gcgatacggt
     3841 cgaagagcgg tatctggatg atgccgagga acttgagccc gaacaggagc acgagcaccc
     3901 cgccgaacag gagcaggtag tggttgtagg tcttgatgag cccgccgagc gcggcggcgg
     3961 cggcgccgaa gagggcgaag acgatgacga tgcccagcga gaaggcggcg gcccgcagga
     4021 acagcctcgc gccggagggg cggtcgccgg ggcgctccat acccgccacc gcggcgagat
     4081 agaccgggat cagcggcagt acgcaggggg tgaaaaaggt cgcaagcccg gcggtgaaca
     4141 gcgcggtcag cgaaaaggtc atcggtctct ccgttcccga tagttccgat aggtataatg
     4201 cccgatcgcc tcaagtcaag cgggaaggga ggacccgacc ggcgcgacac gccgaataaa
     4261 ttgactggcg tcccgaaagc cgctaggatg accgcgtact gtttgtaacg ggcgggcatc
     4321 atgaagaatg tctttttctt cgcgctgttc ctttttgcct tctacggcgt ctatacgctc
     4381 ctccactggt tcttctggcg cgggcttgtc gcggcgttcc ccctgccgct ctgggcgcgg
     4441 cgcggggtca tcgtcttttt cgcgctgatg ttcctgttcc cgatagcgaa tcacgcgagc
     4501 agcgcgctcg ccgggacgct cccgtgccgc ggcgtcgcgt ggatcgggtt cctgtggctc
     4561 ggctttttgt tctactacct gttcgtcggc ggtgcgttcg aactcgccgc cttcgcggtg
     4621 aagcgcttcg ccgaagagga actgtcgcgc cgcgcgctct ttatcgccgc ttcggcgctc
     4681 agcgtcatgg tcatcgccta cggcttccat gaggcgtcgc agatacgggt gcgccaccat
     4741 accctgacga cggcgaaact gcccgaattc atcgccaaga tacgcatcgt gcaggtgagc
     4801 gatgtccatt tcagccagtt gcacggggtc gccttcgccg accgcctgac cgcgctcatc
     4861 gccgaacagc ggcccgatct tctcgtgatg accggcgatt tcctcgaccg cgggctggtg
     4921 gacgaggagg ggatcgccgc gaagtggcag gcgctcgaag cgccgctcgg caaatacgcg
     4981 gtgacgggca accatgaatt ctacaacggc atcgaccatt cggtcgcgct gatagagaag
     5041 tcgggcttca cgctgctgcg caaccgcggt gagacacgcg ccaacaacgc ctttgtcatc
     5101 gcgggggtgg atgacccggc cgcggcgcgg ttcggcgagg agccgccgac cgccaaacgg
     5161 gtgctcgagt cgctcccttc caagccctac cggatattcc tgcggcacca gccgaaggat
     5221 gacccggaga ccgcggggct cttcgatctc cagttctcgg gccacaccca caacgggcag
     5281 atatggccct tttcgctctt cgtctcgata ccgttcccga tgcgcggcgg catctatcag
     5341 cagaaagagg gcgggaccac cgtcgtgtcg agcggcgccg ggacctgggg gccgccgata
     5401 cgggtgctcg cgccgcccga tatcgtggtg gtcgatctgg tcaggaaagt tgaataacct
     5461 cttgcagcgg acaccgacat ggaacacctg aaactgctca tcatcggcgc cggtccgggc
     5521 ggctatgtcg ccgcgatacg ggcggcgcaa ctcggtcttt ccccggtcgt cgtcgaaagg
     5581 tcgaacgatc tgggcggcac ctgcctcaac tggggctgta tcccggcaaa ggcgctcatc
     5641 cgcgccgccg aggcgttcga ggacgcgcag gaggcgtgcc ggttcggcgt ccacgccgag
     5701 ccgtcgttcg tctggagcga ggtggtcgcc cacgcgaagg gggcggtcgc gaagaacaac
     5761 aaggggatcg agttcctctt caagaagcat aacatccggc tcatccgcgg cgcggcccgt
     5821 ttcaccggcc ccaaggagat cgcggtgggg gacgaccgct acaccgccga ccatatcatc
     5881 atcgccgccg ggtcgatcgt gaaggacctg ccgcatatca cggtggacgg cgacggtgtc
     5941 tggtcgagcg accatgcgct gttctgcgag aagttgccga aaagcgtcgc gatcatcggg
     6001 gcgggcgcca tcggcgtcga gttcgcctat atcttttcgg tgttcggcgt tgaaacgcat
     6061 ctcatcgagg cgctcgaccg gttcgtcccg ctcgaggacg gcgaggtgag caaggaactg
     6121 tacaaggagt tccgcaagcg gaagataaag tgttaccccg gtacctgcgt cgataaggcc
     6181 gaacaggggc cggaggggat gacgctcacg ctggcgggcg gcaagagcgt cacggtggag
     6241 aagatactct cggcggtcgg ccgccgcccc ggcagcgggt cgctcgggct cgatgcggcg
     6301 ggtatcgcga ccgatccgcg cgggttcatc ccggtgaacg aacactacca gacgacggtg
     6361 cccggggtgt acgccatcgg cgaccttatc gcgacgccga tgctcgccca caccgccgaa
     6421 cacgagggga gccacgcggc cgaacacatc gcggggctgc atccccaccc gatagattac
     6481 cggcagaatc ccgggtgcac cttctgcgaa ccggccatcg cgtcggtcgg gctgaccgag
     6541 gagcgggcga aggaactggg cgtcgcctac gccgtcggga aattcccgtt ctcggccaac
     6601 ggcaaggcgg tggcgtccgg ggcgaccgcc ggcttcgtca aggtgcttat cgccgccgac
     6661 agccacgaac tgctcggcgc ccacatcatc ggacgcgggg cgaccgatct catcgccgaa
     6721 ttcctcccgg cgctccggct gaaggcgaag gcggaagata tcgtagaggc catccacccg
     6781 catccgacgc tctgcgaggc gtcgctcgaa gcggtgctcg acgcgctcgg ccgcgtcatc
     6841 cacaagtaac ggggaaaaga cgaccgtgga tgcggtgacg gtcatagact tcgagacgac
     6901 gggactcgag ttcgccaagg aggaccgcat cgtcgagatc ggcgcggtca aatggcgcga
     6961 ccatcgcatc accggcatat acgacgcgct ggtcaacccc ggccgtccga taccggccga
     7021 agtggtgcgc gtccacggca tcaccgactc gatggtggcc agcgccgccc cgctcgatgt
     7081 cgccgtcccc gaactgctcg acctcatcgg cgacgatctg gtggtgatgc acaatgtcga
     7141 attcgacagc ggctttctca aggcgcagat gaaactgctc ggcatcgagc gcgaactgac
     7201 ctattacgat acgcttcccg cggcgcgcaa acggttcctc gggtcgccca actaccggct
     7261 cgcgacgctc aagaacttcc tcaatttgaa gcagttgggg accatgcacc gggcgctcgc
     7321 cgacgcctat gtcaccctgc aactctacct gaaacttgag aacctctggg cgatcgattc
     7381 gaggagtcaa ctggaaaagc aactgaggga gcttacccag cggcgcgcgc cgggggcgcg
     7441 ggtcgtcacg atgtgatatg gtcggtcagg gcttcttgtc ttcgagcgcc cgccggtaga
     7501 gttcgacata ccgtttcgcg ctctcttccc aactgaaatc cttcgccatc gcctcgcggc
     7561 gcatcgcggc gaaatgggcg gggcggtcgt accaggtgct caccgcccag ccgacggtgt
     7621 cgtacaccgc ttcgggcgag aggtactgga acttgaaacc ggtgccggcg ccggtcttct
     7681 cgtcgtactg ttcgaccgtg tcctcgagcc cgccggtcgc ccggacgatg gggagcgtgc
     7741 cgtagcggag cgagtatatc tggttaagcc cgcacggttc gtactgcgag ggcatgagga
     7801 acaggtcgct ccctccctcg atgatgtgcg agagttcgtc gctgtagccg atgtaggcgc
     7861 cgacgcggcc gggatagcgc gccgccagtg cgccatagta ctgttccagc actttgtcgc
     7921 ccgcgccgag gacggcgaac tgcaccacca tgtcggcgag gatgcggtcg agcgccgacg
     7981 cgaccacatc gagccccttc tgcgcgacga accggctgac gatgccgatc accgggaccg
     8041 tcgggtcgac cgcgagcgcc aaggtgcgct gaagcagttc cttcgccgcc gcctttccgg
     8101 cgaggtcgtc ggccgagtag gggaggggga ggtagcggtc gttcccgggc gaccagcggg
     8161 catagtcgac gccgttgagg atgccgaggt agcgatcccc tttcgcgctc aggaagggcg
     8221 ccagcccgca cgacaacggg ccggaaagcg tctctttcgc gtaggtcggg ctcacggtgt
     8281 tgacccggtc ggcgaagtgg atgcctccct tgaggtagtt cacgcggccg tggtcctcga
     8341 atatgtcggg cttgaaatgt tcccaccgga gcccgctgta gtcgtaatgt tcggtgccgt
     8401 acaccccctg ataggcgagg ttgtgtatcg tgaggatgcc cgccgccttg gcaagcagcg
     8461 ggtcgttcca gtgccagacc ttgaggtacg ccacggcggt cgccgcctgc cagtcgtggg
     8521 cgtggacgat gtcgggcatc cagtacaggt cgcgcaggag ttgcagggcg gcgcggctga
     8581 agaaggcgaa ccggcgcggg ttgtcgggat agtcgttgaa ccacgcgtcg tggtacagtc
     8641 cgtcgcgctg gaagtatttg ttgtgctcga tgaagtagac cgggaccttg ccgtcgcccg
     8701 tcgcctgatg cacggcgcac cattcgagcg tgttgcccat ccagaccccg agttgctcca
     8761 tgcaggggct cagacggtgt ttttcctgat cgatgcaccc gtagaggggc aggatgacga
     8821 cgacctcatg gccgagtccg gcgagcgcct gcggcagggc gccgacgaca tcggcgagcc
     8881 cgccggtctt cgcgtagggg acgcattcgg aggatatcat ggcgatcttc ataggggtct
     8941 cccgccggtg gtcatgttga tcacgggggt acggtatctt tctttgggcg gattacaatt
     9001 tttttgtgcc cgacgaaatt gcaaaacggg gcggttttcg ctatgctgtc ctgtttttcc
     9061 ggcccccgcc ccttttttac ggagacggtc atgtggttct acgaacagta cgacgagaag
     9121 gcgtcactgg gtctcaaggt ccggcgcaaa ctgcatgagg agcagagcaa gttccagaag
     9181 atagcggtgt tcgagaccga gttcttcggc aatctgctga cgctcgacgg gctggtgatg
     9241 ctgtgcgaga aggacgagtt cgtctaccac gagatgatca c
//
LOCUS       DAOQUE010000045         9219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8367_length_9219_cov_4.479921, whole genome shotgun sequence.
ACCESSION   DAOQUE010000045 DAOQUE010000000
VERSION     DAOQUE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8367_length_9219_cov_4.479921"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..670
                     /locus_tag="PKH10_02420"
     CDS             119..670
                     /locus_tag="PKH10_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcaF family extracellular polysaccharide
                     biosynthesis acetyltransferase"
                     /protein_id="HNT27011.1"
                     /translation="MSNTIDLSKFNNGWYKPGCILRRALWFIKGRVIINTYFPWPMWV
                     KRWTLRFFGAKLGHGVVIKPKVNIKYPWFLTVGDNSWIGEGVWIDDLGKVTIGANCVL
                     SQGAMLLTGNHDYKKESFDLIVGDITLEDGAWIGAQAVVCPGVTVRSHAILTVGSVAT
                     KELDAYGIYQGNPAAKVRERVIQ"
     gene            complement(920..2875)
                     /locus_tag="PKH10_02425"
     CDS             complement(920..2875)
                     /locus_tag="PKH10_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27012.1"
                     /translation="MARPAPLIILLSTLLVGCAAGKETVWPTPEHPEEEPNNDPPGAT
                     IIEEAVTYEGVIDDERPATSDTDHYRLLGHAGFIYRVEFETLDPDLLPLIEIADNGGL
                     LKEFRPPGNARSVVEFFEPAEGNLILRVSDEPTRRNTPETWEDFRYWLRVTKRWACEY
                     PASGDLAAGKPLAFDMEKPPAVPRLFTIDATAGHYAVAVASERLDRDKKLVAMDCVTG
                     EVIGGSDDRDPESGLTDPYWYEHFGAPRDMRLIVERWSDAAGDRAPDKKGDPTTLTLT
                     RHDPSHELEPNDIVEYANVCDPAGTAGELDAEQKVIAAVLQDDRDWFKHETTRGAVAR
                     FALSAGAAGEMQVSLGSSSTDMGGYTLLSIRSLAVSFDAAGVAEATAFLPFTGTLYLG
                     LQGSGMGYEFTYEEDEAIAALEGVGAWPLELPHCRGAYRTWRFPPFVNAVRLSLDSSD
                     GDIAWRIYNGRRYPLIELGAAGAGAENVWYLTRLSDETDILFAVEPSRCDAAATQEAT
                     LTVTEAPFMTEEPDLTTTDLVVEAVPGTTYIGWFDTDVPIIENRWTFTPGTDGTLFLM
                     TTPVKERGGATVDTVIRLYEDGNDTPIAQNDDMIEGISFSPYSFLRHPLKGGATYTIS
                     VKPFMDDSSNIAAMNIHANFGLDVRFE"
     gene            complement(2854..3354)
                     /locus_tag="PKH10_02430"
     CDS             complement(2854..3354)
                     /locus_tag="PKH10_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNT27013.1"
                     /translation="MTVPNPAPAGRHDRWLTALIVLVTALLIGYNLVMFYGQAYFLKG
                     RQVSSLLLADPQSGAEKEVVVPGGAVLLNFWATWCSSCVAELPVLTEAGIPVVGILKK
                     PVRADHLRDMKLPWENLVGSDDLFDEFMVSAVPVTMFLRDNRIVAVHTGPLTREVVRE
                     WLALPR"
     gene            complement(3361..3801)
                     /locus_tag="PKH10_02435"
     CDS             complement(3361..3801)
                     /locus_tag="PKH10_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNT27014.1"
                     /translation="MDRTSAEESTVVDVDLRKLFSETRTVAIVGLSDNPVRTSHRIAK
                     YLKNEAGYRIIPVNPTVAEVLGEKSYPDLLSIPADIVIDIVNVFRRSEHLEAVAREAV
                     QRGCRFFWSQLGVYSQKAEEVLRDAKLPFVMNRCIFVEHQNLFH"
     gene            complement(3850..5559)
                     /gene="argS"
                     /locus_tag="PKH10_02440"
     CDS             complement(3850..5559)
                     /gene="argS"
                     /locus_tag="PKH10_02440"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNT27015.1"
                     /translation="MKIIEKHLEERQGKRIRALLPQAPLDKLFVTRTGDEQFGDYQTN
                     FAMAAAKDLTMKPMDIAKRIVSEQGRSDLGELSIAGPGFINLTLSNDFIAEEVAKIGR
                     EPYDFSFLDRAGSVVIDYSSPNIAKPMHIGHLRSTVLGDSIARIYRYLGYTVVGDNHL
                     GDWGTQFGKLIVAYRRWLDRAAYEKSAIEELERIYVLFSQATESDPSLEEEARAELKK
                     LQDGDPENRKLWREFIDLSLAEYNRIYERMGVRFDTYHGESFFHDRMPAMLDLLIEKG
                     LAKESEGALVVFFDEKEKLHPCIVRKGDGAFLYATSDLACLQFKRETYTLNRAVYVTD
                     ERQQPHFKQIFRIARMLGWQERLDHVWFGIMRFGDDTFSSRAGNVIKLDELLNEAVRR
                     ARIVIEEKNPELPEDEKAAVAEMVGLGAVKYADLSQNRISAVSFEWDKALSFDGNTAP
                     YLQYTHARIRSIARKAAETGRPADPDAKIAITGPTERRLAVYLLGFGTVVQKAAENYR
                     PNLIADYLFELGQRFNTFYNANPVLKENDAVMQSRLALCLRTAHVVKEGLSLLGIGAP
                     ERM"
     gene            complement(5579..5908)
                     /locus_tag="PKH10_02445"
     CDS             complement(5579..5908)
                     /locus_tag="PKH10_02445"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNT27016.1"
                     /translation="MIRGIGIDIESVERFRNAEPGLLDRVLSSDDRAYVEKFADPAPH
                     IAGIWAAKEALVKAIGRKDLRFDAVSVRHEATGRPYLSYTPDEGTLHLSISHGGGQAV
                     AVVVWDA"
     gene            complement(5905..6624)
                     /locus_tag="PKH10_02450"
     CDS             complement(5905..6624)
                     /locus_tag="PKH10_02450"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNT27017.1"
                     /translation="MHKLGVNIDHVATLRQARGTRYPDPVFAALIAEQAGASNITLHI
                     REDRRHAQERDLELIRKSVSIKVNLEMAPTADLKAIALRHRPDSCTLVPEKRHELTTE
                     GGMDLLSDAYPGLKAYIAELRKAGIVVSLFIDPDEKQVTAACKLGARAVEFNSGRYAE
                     AKGDKEAAREIERIRQATVFALSNDLTAHVGHGIHYHNVRPLVEMQLLEEFNIGHAIV
                     ARAVMVGFERAVADMLALLEP"
     gene            complement(6647..>9219)
                     /locus_tag="PKH10_02455"
     CDS             complement(6647..>9219)
                     /locus_tag="PKH10_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27018.1"
                     /translation="EKIDEKSDIYSLGAVLYEILTGQPPHTGYTVFEILAKVLNDDII
                     DPQKRDDRIPRELGAIARKALKKKKEERYPSALAIAADIESYMSGGRVGAYEYSSWEL
                     LMRFILRNKTISGLATLIFLLSIFAAVLISGYYREALVEKQRAEDNEREVHYQLSQFL
                     TEKSQRAYQAKDLLSARMFAASALVHSPYNPYGTNRFTAPDQMRDEKALADLVEAHSA
                     LFQATAFGLATFAGVYLDQDYWTQSAVFSPDGRRLASGSSTGTIKLWDMSSKRLIASI
                     ANNGGVVYAVVFSPNGRYFVSAHEDKIIRFWDTDTRELIGSLAAHDDKVRAIAFSPDG
                     RFLASGSDDGTVKVWDAATKKNVAVFAGHRDKVISIAFSPDGRMLASGSFDKTIRLWD
                     VATKKNIAVLTGHDDWVPGIAFSPDGRILASVSNDRTVKLWDVGEKALLTTFTGHEEV
                     VLALAFSPDGSLLASAGGDRTIRLWSMTGKNLAMTFTAHNDTIGALAFSPDGRSLVSA
                     GYDRTLKLWDISENKNIVTLKGHGRSVSSLAYSSDGRFLATAAYDDTIRLWDLAENRQ
                     IAVLAGNGDKINAIAFSQKGDLLASGGWDKKARIWDVGTRELLWISPAHADTVWSVAF
                     SPDGKMLATGSADKTIKLWDLTGERKLLPALSGSGHGGVVFKVVFSADGRHLASASWD
                     NTAKLWDVGSKQLVRTLEGHTDILGGISFSPDDRLIASSSKDNTIKLWRSDTGELIRT
                     IEGHKAMINHVEFSSDGRFLLSTGDDRTARIWEVESGTGLQTIGLPEMGLWASFSRDG
                     KHFAVNSGNDVLIIPIDLDYWKRDPHELLKEAEKAAGMKLDGFTLHPMTPADFEAYRQ
                     "
BASE COUNT         1691 a         2811 c         2868 g         1849 t
ORIGIN      
        1 gaagcggacg ccttgcggag ctccgcgcca tacagcgcat ccgcagtcct cggcggaacc
       61 gtccggcccc ttgtcaaggg ggccttgacg gggtttgtaa aataacggga ggcaatagat
      121 gagcaacacg atcgatcttt caaagttcaa taacgggtgg tacaagccgg ggtgcatcct
      181 gcggcgggcg ctgtggttca tcaaggggcg ggtgatcatt aacacctatt tcccgtggcc
      241 gatgtgggtg aagcggtgga cgcttcggtt cttcggcgcg aaactggggc acggggtggt
      301 catcaaaccg aaggtgaaca tcaagtatcc gtggttcctg acggtcggcg acaactcgtg
      361 gatcggcgaa ggggtctgga tagacgacct cgggaaggtg acgatcgggg cgaactgcgt
      421 gctgtcgcag ggggcgatgc tgctcaccgg caaccatgac tacaagaaag agtcgttcga
      481 tctgatcgtg ggcgacatca cgctggagga tggggcgtgg atcggggcgc aggcggtcgt
      541 ctgcccgggg gtgacggtgc ggagccacgc catactgacc gtagggtcgg tcgcgaccaa
      601 ggagttggat gcctacggga tatatcaggg gaatccggcc gcgaaggtgc gcgagagggt
      661 cattcaataa ccggcccctg ccctgtcaag ggaagggaag aaagggatgg gtttaattga
      721 tagaagtttc ggcgagcgcg atgctcttcg ccgaccccat ttcttctttg taacggcaaa
      781 gaaaaaatgg gggaaagaag aaagccgtta ccagaagcgg acgccttgcg gagctccgcg
      841 ccatacagcg catccgcagt cctcggcgaa aaacaacctt ccccttcctt gtcaagggaa
      901 gggaattaaa gggaagggtt tactcgaacc tcacatccag tccgaaattc gcgtggatgt
      961 tcatcgcggc gatattgctc gaatcgtcca tgaagggctt caccgatatc gtgtaggtcg
     1021 cgccgccttt cagcggatgc cgcagaaaac tgtaggggga gaacgatatc ccctcgatca
     1081 tatcgtcgtt ctgggcgatg ggcgtgtcgt taccatcctc gtagagccgg atcaccgtgt
     1141 cgaccgtcgc gccaccacgc tctttcaccg gcgtcgtcat gaggaagagc gtcccgtcgg
     1201 tccccggcgt gaaggtccag cggttctcta tgatcgggac atcggtgtcg aaccagccga
     1261 tgtaggtggt gcccggcacc gcctcgacga cgaggtcggt cgtcgtgaga tcgggctctt
     1321 cggtcatgaa cggcgcctct gtcaccgtga gcgtcgcctc ctgcgtcgcg gcggcgtcac
     1381 accgcgacgg ctcgacggcg aacaggatgt cggtctcatc cgagagccgc gtgagatacc
     1441 agacattctc cgcgcccgcc cccgcggcgc cgagttctat cagcgggtag cggcggccgt
     1501 tgtagatgcg ccacgcgata tcgccgtcgg acgagtcgag tgacaaccgg accgcgttca
     1561 cgaaaggggg aaagcgccat gtccggtagg cgccgcggca gtgtggcagt tcgagcggcc
     1621 acgcccccac cccctcgagc gccgcgatgg cctcatcctc ctcgtaggtg aattcgtagc
     1681 ccatgccgct cccctgcagg ccgaggtaga gcgtcccggt gaacggcagg aaggcggtcg
     1741 cctccgccac gcccgccgca tcgaacgaaa cggcgagcga ccgtatcgag agaagcgtgt
     1801 agccccccat gtcggtcgag gaactgccga gcgacacctg catctcaccc gccgcaccgg
     1861 cgctcagtgc gaaccgcgcc accgcgccgc gggtcgtttc gtgcttgaac cagtcgcggt
     1921 catcctgcag gaccgccgcg atgaccttct gctccgcgtc gagttccccg gcggtcccgg
     1981 ctgggtcgca gacattggcg tattcgacga tatcgttcgg ttcgagttcg tgcgacgggt
     2041 cgtgccgcgt gagcgtgagc gtcgtcgggt cgcccttctt atcgggggcg cggtcgcccg
     2101 ccgcgtcgct ccaccgctcg acaatgagcc gcatgtcgcg cggcgcgccg aaatgttcgt
     2161 accagtaggg atcggtgaga ccgctctccg ggtcgcggtc gtcggagccg ccgatgacct
     2221 cgccggtcac gcagtccatc gcgaccagtt tcttgtcgcg gtccagccgt tcggaggcga
     2281 ccgccaccgc gtagtggccc gccgtggcgt cgatggtgaa aagacgcggg accgccggcg
     2341 gcttttccat gtcgaacgcg agcggtttcc ctgcggccag atcgcccgac gcgggatatt
     2401 cgcaggccca acgcttggtg acgcggagcc aataccgaaa gtcttcccac gtttccggcg
     2461 tattgcgccg ggtcggctcg tcgctcacgc ggaggatgag gttgccttcc gccggctcga
     2521 agaactcgac gacgctccgg gcgttaccgg gcgggcggaa ctccttgagc aggccgccgt
     2581 tatcggctat ctcgatgagc ggcaggaggt cggggtcgag cgtttcgaac tcgacgcggt
     2641 atatgaagcc agcgtgaccg aggagccgat agtggtcggt gtcgctcgtc gcgggccgct
     2701 cgtcgtctat caccccctcg taggtgaccg cctcttcgat gatggtggcg cccggcggat
     2761 cgttgttcgg ctcctcctcg ggatgttcgg gcgtcggcca gacggtctcc tttccggcgg
     2821 cacagccgac aaggagcgtg gaaagcagta tgatcagcgg ggcagggcga gccattcgcg
     2881 caccacctca cgcgtcaggg gaccggtatg gaccgcgacg atgcggttgt cgcgcaggaa
     2941 catcgtaacc gggacggccg acaccatgaa ctcatcgaac aggtcgtccg acccgacgag
     3001 gttctcccac ggcagtttca tgtcgcgcag gtggtcggcg cggaccggct ttttaaggat
     3061 gccgacgacc gggatccccg cctcggtgag caccggcagt tcggcgacgc acgacgaaca
     3121 ccaggtggcc cagaaattca ggagcaccgc gccgccgggg acgacgacct ccttctcggc
     3181 gccgctctgt gggtcggcga ggagcaggga cgacacctgc ctgcccttca ggaaataggc
     3241 ctgcccgtag aacatcacga ggttgtaccc tatgaggagg gcggtgacca gcacgatgag
     3301 ggcggtcagc caccggtcgt gacgcccggc gggcgccggg ttcgggacgg tcatgcggta
     3361 tcagtgaaaa aggttctggt gttcgacgaa tatgcagcgg ttcatcacga acgggagttt
     3421 ggcgtcgcgc aggacctctt cggccttctg gctgtagacg ccgagttgtg accagaagaa
     3481 ccggcacccg cgctgaaccg cctcgcgggc gaccgcttcg agatgctccg aacggcggaa
     3541 gacattgacg atgtctatca cgatgtcggc cggtatcgac agcaggtcgg gataactttt
     3601 ctccccgagc acctcggcga cggtcggatt gaccggtatg atgcggtacc ccgcttcgtt
     3661 cttgagatac ttggcgatgc ggtgcgaggt gcgcaccggg ttgtcagaca ggccgacgat
     3721 ggcgaccgtc ctcgtttcgc tgaaaagttt tctcagatcg acatcgacca cggtgctctc
     3781 ctccgctgaa gtgcggtcca tgttatcata cgccggaccg cttcgcaact aaaatctgat
     3841 aaagatgccc tacatacgct ccggcgcccc gatgccgagg agcgacagcc cctccttcac
     3901 cacatgggcg gtccgcagac agagggcaag gcggctctgc atcaccgcgt cgttctcttt
     3961 gaggaccggg ttcgcgttgt agaaggtgtt gaaccgctga ccgagttcga acagatagtc
     4021 ggcgatgagg ttgggacggt agttctcggc cgccttttgc accaccgtcc cgaacccgag
     4081 caggtacacc gcgagccgcc gctcggtcgg ccccgttatc gctatcttcg cgtcgggatc
     4141 ggcggggcgc cccgtttcgg ccgccttccg cgctatcgac ctgatgcggg catgggtgta
     4201 ctgcaggtag ggagcggtgt tgccgtcgaa tgagagcgct ttgtcccact cgaacgacac
     4261 cgccgatatg cgattctggc tgaggtcggc gtatttcacc gcgccgagcc ccaccatctc
     4321 ggcgaccgcc gccttctcgt cttcgggcag ttcgggattc ttttcctcga tgacgatccg
     4381 cgcgcggcgc accgcctcgt tcagcagttc gtcgagtttt atcacattgc ccgcgcggct
     4441 ggagaaggtg tcgtcgccga accgcatgat gccgaaccag acatggtcga gccgctcctg
     4501 ccagccgagc attcgcgcga tgcggaatat ctgtttgaaa tgcggttgct ggcgttcgtc
     4561 ggtcacatag acggcgcggt tgagcgtgta ggtctcgcgt ttgaactgga gacaggcgag
     4621 atcgctcgtc gcgtagagga acgcgccgtc gcccttgcgg acgatgcagg ggtgcagttt
     4681 ctccttttcg tcgaagaaca ccacgagcgc accttcagac tctttcgcca agcctttttc
     4741 gatgagcaga tcgagcatcg ccggcatccg gtcgtggaag aacgattcgc cgtgataggt
     4801 gtcgaagcgc acccccatcc gttcatagat gcgattgtac tcggccagag aaagatcgat
     4861 gaattcccgc cacagtttcc ggttctccgg gtcaccgtcc tgcagtttct tcagttcggc
     4921 acgcgcctct tcctcgagcg atgggtcgct ttcggtcgcc tgcgagaaca ggacatatat
     4981 gcgctccaat tcttctatcg cgctcttctc gtacgcggcc cgatcgagcc agcgacggta
     5041 ggcgacgatg agtttgccga actgcgtccc ccagtcgccg agatggttgt cgccgacgac
     5101 cgtgtacccg agataccggt aaatgcgggc gatgctgtcg ccgagcacgg tcgagcggag
     5161 gtggccgatg tgcatcggtt tggcgatgtt gggcgacgag taatctatca ccaccgatcc
     5221 ggcgcggtcg aggaacgaaa agtcgtaggg ctcgcggcct atcttagcga cctcttcggc
     5281 gatgaaatcg ttggagagcg tcagattgat gaagccgggg cccgctatcg aaagttcgcc
     5341 gaggtccgaa cggccctgct ccgacacgat gcgtttcgcg atatccatcg gtttcatcgt
     5401 gaggtctttc gccgccgcca tcgcgaagtt ggtctggtaa tcgccgaact gctcgtctcc
     5461 ggtcctcgtc acgaagagtt tgtccagcgg ggcttgcggg agtagtgctc gtattctctt
     5521 gccctgccgc tcttccagat gtttttcgat gatcttcatg cggtcctctt ccgttgcttc
     5581 aggcgtccca gacgaccacc gcgaccgcct gaccgccgcc gtgcgatatc gaaaggtgga
     5641 gcgtcccttc gtcgggagtg taggaaagat agggccggcc ggtcgcctcg tgccgcaccg
     5701 acaccgcgtc aaaccggagg tctttgcggc ctatcgcctt gacgagcgcc tccttggcgg
     5761 cccagatacc ggcgatgtgc ggcgcgggat cggcgaactt ctccacatag gcgcggtcgt
     5821 cggacgacaa cacccggtcg agcaggcccg gctccgcgtt gcggaaccgc tcgacgctct
     5881 cgatgtctat accgatgccg cgtatcatgg ttcgagcagg gcgagcatgt cggccaccgc
     5941 ccgttcgaac ccgaccatga cggcgcgggc gacgatggcg tggccgatgt tgaactcctc
     6001 gagcagttgc atctcgacga gcggccggac attgtggtaa tggatcccgt gaccgacatg
     6061 ggccgtcaga tcgttggaca gcgcgaacac cgtcgcctgc cggatacgct ctatctcgcg
     6121 cgccgcctcc ttgtcgccct tggcttcggc gtagcggccg ctgttgaact cgaccgcccg
     6181 cgcccccagt ttgcacgccg cggtgacctg tttctcgtcg ggatcgatga acaggctcac
     6241 gacgatgccc gccttcctga gttcggcgat gtaggccttg agcccgggat aggcgtccga
     6301 gaggaggtcc atcccccctt cggtggtgag ttcgtgccgc ttttcgggga cgagtgtgca
     6361 actgtcgggg cggtggcgca gggcgatcgc cttgaggtcg gcggtcggcg ccatctcgag
     6421 attgaccttt atcgaaacgc tcttccgtat aagttcgagg tcccgttcct gcgcgtggcg
     6481 acgatcctca cggatgtgga gcgtgatgtt ggatgcccct gcctgctcgg cgatgagcgc
     6541 cgcgaacacc gggtcggggt agcgcgtgcc gcgtgcctgc cgcagtgtcg cgacatgatc
     6601 gatgttgacc cccagtttgt gcatgaccat cacctcctcg ttccttctat tgacgatagg
     6661 cctcaaaatc cgcaggcgtc atgggatgca gggtgaagcc atccagtttc atgccggccg
     6721 ccttctccgc ttccttgagc agttcgtgag gatcgcgttt ccagtaatcg aggtctatgg
     6781 gaatgatgag gacatcgttg ccgctgttga ccgcgaagtg ttttccatcg cgggagaaag
     6841 atgcccacag ccccatctcg gggagcccga tggtctggag acctgttccg ctttcgacct
     6901 cccatatccg tgcggtgcga tcatcaccgg tcgaaaggag gaatcggccg tcggacgaga
     6961 actcgacatg gttgatcatg gccttatgtc cttctatcgt tctgataagt tcgccggtat
     7021 cgctcctcca cagtttgatg gtgttatcct tgctcgacga ggcgatcagg cggtcatcgg
     7081 gggaaaatga aatgccacct aagatatcgg tatgaccctc cagcgtccgg accagttgtt
     7141 tcgagccgac atcccagagt ttcgcggtgt tatcccaact cgccgaggcg agatgacggc
     7201 catcggcgga gaacacgacc ttgaacacca cccccccatg accggaaccg gaaagagcgg
     7261 ggaggagttt tctttccccg gtcaggtccc atagtttgat ggtcttatcg gcgcttcccg
     7321 ttgccaacat ctttccgtcc ggagaaaagg cgaccgacca caccgtatcg gcgtgcgccg
     7381 gggagatcca caggagttcg cgggtgccga catcccagat ccgcgccttt ttgtcccagc
     7441 cacccgatgc cagcagatca cctttctgcg agaaggcgat cgcatttatc ttgtcaccgt
     7501 tgcccgcgag cacggcgatc tgccggttct ccgcgagatc ccacagccgg atcgtgtcat
     7561 cataggccgc agtggccaga aaccgcccat ccgacgaata ggcgagcgag gagaccgatc
     7621 ttccgtggcc tttcagcgtg actatattct tgttctcgct gatatcccac agcttaagcg
     7681 tccgatcgta acccgccgag accaaggagc gaccgtcggg cgagaaagca agcgctccta
     7741 tggtgtcatt gtgcgcggtg aaggtcatgg caaggttctt tcccgtcatg ctccacagcc
     7801 gtatggtccg gtcaccgccc gccgaggcca acagtgagcc atcgggcgag aaagcaaggg
     7861 cgaggaccac ttcctcatga ccggtgaagg tcgtcagcag ggccttttct cccacatccc
     7921 acaacttcac ggtgcgatcg ttgctcaccg acgccagtat ccgaccgtcg ggcgagaagg
     7981 cgatcccggg cacccaatca tcatgaccgg tcaggacggc gatatttttc ttcgtagcga
     8041 catcccagag ccgtatcgtc ttatcgaaac tgcccgacgc aagcatccgg ccatcaggcg
     8101 agaaggcgat cgatatcacc ttatcgcggt gaccggcaaa aaccgcgaca ttttttttcg
     8161 tggccgcgtc ccacaccttc accgtcccgt catcgcttcc cgacgcaaga aaacggccat
     8221 cgggcgaaaa ggcgatggcc cgcaccttat cgtcatgtgc cgccagtgac ccgatcagtt
     8281 cccgagtatc ggtgtcccag aaccgtatga tcttatcctc atgagccgaa acgaaataac
     8341 gcccattagg cgaaaaaaca acggcgtaca cgactccgcc gttgttcgcg atagaggcga
     8401 tcagccgttt cgacgacata tcccagagtt taatcgttcc cgtgctgctt ccggacgcca
     8461 gtctccggcc gtcgggggaa aaaacggccg actgtgtcca gtaatcctga tcgagataga
     8521 cgccggcaaa ggtggccagc ccgaacgccg tggcttggaa cagcgccgaa tgggcctcga
     8581 ccagatcggc gagcgccttt tcgtccctca tctgatcggg agcggtgaaa cggttggtgc
     8641 catacgggtt ataggggctg tggaccaacg ccgaggcggc gaacatgcgg gcgctgagaa
     8701 ggtccttggc ctgataagcc cgttgtgatt tctcggtcag gaactgcgac aactggtagt
     8761 ggacctcccg ctcgttgtcc tcggcccgct gtttctccac cagcgcttcc ctatagtagc
     8821 cgctgatgag caccgcggcg aagatcgaca gcaggaatat cagtgtcgcg agaccgctga
     8881 tcgtcttgtt ccggagaatg aagcgcatga gcagttccca acttgaatat tcgtatgccc
     8941 cgacccggcc gccggacatg tacgattcga tatcggcggc gatggcaagc gccgacggat
     9001 aacgctcctc ctttttcttc ttcagcgcct tccgggctat cgcgccgagt tcgcggggta
     9061 tccgatcatc ccttttctgc ggatcgatga tatcgtcgtt cagcaccttg gcgaggatct
     9121 cgaacacggt atatccggta tggggcggct ggcccgtcag tatctcgtac aacaccgcgc
     9181 ccaaggagta gatgtcactt ttctcatcta tcttctcta
//
LOCUS       DAOQUE010000046         9212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8381_length_9212_cov_4.294747, whole genome shotgun sequence.
ACCESSION   DAOQUE010000046 DAOQUE010000000
VERSION     DAOQUE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8381_length_9212_cov_4.294747"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /gene="dnaK"
                     /locus_tag="PKH10_02460"
     CDS             <1..1364
                     /gene="dnaK"
                     /locus_tag="PKH10_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNT27019.1"
                     /translation="IDKKGKNMKIAVYDLGGGTFDISILELGDGVFEVLATNGDTFLG
                     GDDFDQRIIEWLVDQFRKESGIDLSKDAMALQRLKDAAERAKHELSSAMETTINLPFI
                     TADQTGPKHLNITMSRAKLENIVEDLAKGTLGPCEKVLKDAKVSASDINEVILVGGMT
                     RMPMIQRLVKEFFGREPHKGVNPDEVVAIGAAIQGGVLKGDVKDVLLLDVTPLSLGIE
                     TLGGVFTRLIPRNTTIPTKKTEVFSTAVDNQPAVTVRVFQGEREIAVENKLLGEFDLQ
                     DIPPAPRGMPKIEVSFDIDANGILNVSARDIGTGKQKNIVIKAGSGLSDDEIKRMVSE
                     AEKFKAEDEKRKTLVEARNNAEQLIYSTEKSFKEYGDKLSDEEKRDVEEKIADLRGVL
                     DKDDLEVIKGKTQALGQSAYKIAEKVYRGAGTEQGKDPGAEPSDDDKGDDKVVDADYE
                     PKK"
     gene            1381..2529
                     /gene="dnaJ"
                     /locus_tag="PKH10_02465"
     CDS             1381..2529
                     /gene="dnaJ"
                     /locus_tag="PKH10_02465"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNT27020.1"
                     /translation="MADLYGILGVRRDATLDEIKKAYRQLAIKYHPDRNKGDKAAEEK
                     FKEINQAYEVLSDAEKRKMYDQFGEDGIKGSPFSAGAGGFDFSDLFSGNFGGGNAGGG
                     LGDIFDEFFGGGARRRGRTAARQGSDLLVTVTLSFDEAYLGTRRDVKVARSGRCAKCA
                     GSGAEPGASRKTCPTCQGRGEVRVSHGFFQMAQTCPSCQGQGSIIEKPCKACQGTGYV
                     REERELSVSIPAGIDTGQKIRMASEGNAGANGGPRGDVYLNVQVKDHPLFHRDGNDIY
                     LELPVSYAQLTLGDTVEVPTMEGKVDLKVPSGTQPDTKMRLREKGFPAVQGRGRGNQF
                     VVLRLEVPRNISSKHRKIVEELKGFDGELKERPFLKDFAEKVKRTFSL"
     gene            2675..3040
                     /locus_tag="PKH10_02470"
     CDS             2675..3040
                     /locus_tag="PKH10_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27021.1"
                     /translation="MRSKSLVIGVLFFFLLCVGCTEEKPAVDVNSFATDCLKNCAMAF
                     NGCMKLSKSPEASKQCEVFFDKCKNDCSAKEKPAPVAAPAPAAEPAAAAAPAPAAAPA
                     PAAAPAPAPAPAPAPEAKQ"
     gene            3137..3409
                     /locus_tag="PKH10_02475"
     CDS             3137..3409
                     /locus_tag="PKH10_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT27022.1"
                     /translation="MSVTIEKLSDIEIQKRGIRQWPVWEKEISTFPWFYDSQEQCLFL
                     DGEVVVTDSDSGKKYHIRRGDFVTFPAEMSCTWEVRQPIKKHYRFE"
     gene            3416..4423
                     /locus_tag="PKH10_02480"
     CDS             3416..4423
                     /locus_tag="PKH10_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT27023.1"
                     /translation="MGRDRLMIAALDEGRLIIPDHRCFFLVRRAAVRTGKNSSRYIDL
                     ELTDGVSTVNGKVWEVTDDIERATETGSIIKVTNGRVAKYQNSLQVIIEDALPVSPEE
                     LDALPGIIPESKLGPAELMARWRALADGLDAGRRAVITEFEASGRVWDLFRSIPAGKS
                     MHHNYRRGLWEHSLQVTELARDIAARYEKRYPVSVPNIILGGLLHDVGKVFEFQVNAS
                     TSIVEKYSDRGRLLGHVYMGASFIEKILKKALPDDADLQMELLHIMLSHHGEYEFGSP
                     KKPKTMDALIIALADNLDANLDAVNIGFNGDMEDRWTKQIFSLQRPFYRTATTVSEDN
                     E"
     gene            4453..5454
                     /gene="gap"
                     /locus_tag="PKH10_02485"
     CDS             4453..5454
                     /gene="gap"
                     /locus_tag="PKH10_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNT27024.1"
                     /translation="MAQKIRVAINGFGRIGRMVFRIGFGRADMEFVTINDLTDAKTLA
                     HLLKYDSVAGIWPHEVKATETAIVVDGKEIPVTAIKDGAQLNYKDIDVVVESTGKYAK
                     KEKAEILRAAGAKKVLISAPANDSDITLVLGVNDKAYDPAKHHVISNASCTTNCYAPV
                     TYIVHKYWKIKKGFMTTIHSYTNDQRILDLEHKDLRRARAGALSMIPTTTGAAKAVGL
                     VIPELKGKFDGISIRVPTPNVSLVDAVYELESEATKEAVNAKFREYANGPLKGILAVS
                     DEELVSKDFNGNPHSGIVDAPYTQVMGGNLLKVLAWYDNEWGYSNRMIDVITKVLMK"
     gene            5574..5882
                     /locus_tag="PKH10_02490"
     CDS             5574..5882
                     /locus_tag="PKH10_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT27025.1"
                     /translation="MTALKKLMVPKKKIVDLCKRWQIREFSFFGSIVGDSFTEASDVD
                     VLIRFDRSTHLGLFEIEAIREELEALLGRPVDIVEMDAVKNPYRRAAILASHEVLYAA
                     "
     gene            5958..7484
                     /locus_tag="PKH10_02495"
     CDS             5958..7484
                     /locus_tag="PKH10_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27026.1"
                     /translation="MKRSVIRLLLVAVFLFSACVPSKNTPNDDDPCAGRCGMVNGVAC
                     GSCSYGLICNTENYCVEMAYDDIPVEEWPDIDQFSCADMKYYANVKAAGFPLKDTKGK
                     ITFCRPGCDTPTENDPQCVKNLWTWINWKRYQEYTTGADTLRECYPWPCELPDMKART
                     NLHECDRNLASQEYIADIGALYDLKIYKSTVGILMTNPHASKAMAYDVTKDAYTSIAH
                     SGHGVGYHYDRFVFYTTYEDYVNSDTGYGNTYIVTAKRNGADYKYEVIYDDAEHMSQF
                     NRPPFVGEKWVLLNVDHTGQGKIEVLYAKVDEWKWHPLKLGQAYEGNIVDNRLTFITD
                     QRQIFVCDLDKLPFDPATECLRIDRAGETAYQPRLNEENKPQLVYFNAAGDNNMTYVD
                     LSGEKPAYSSLPVTISEPQSTGAYPHQFKGDLILYDEAYMPLDNSRLDYKACFYRVDT
                     KKNYCPMKYTDPFLNRYDMGFNSFDGNYQLWKTPAGTIAVVRDIACYCEKEGVCPFEE
                     "
     gene            7565..8749
                     /locus_tag="PKH10_02500"
     CDS             7565..8749
                     /locus_tag="PKH10_02500"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNT27027.1"
                     /translation="MAIKSIRDLAIREKRVFIRTDFNVPMDKQGNITDTTRIEAAIPT
                     IRYAVEHNARVIVASHFGRPDGQRKPEYTMEPVAAKLAELMGIEVTFFEDCIGMGPQQ
                     MVREIKPGQVLVLENLRFYPGEGDNDGAFAKDLAKLCDVYINDAFGVSHRKNASVHAL
                     PLLMETKGMGFLMEKEITELGKLVNFKHGDGFAAILGGAKVSDKIGIIRSLLDSADAL
                     LIGGAMACTFLAAKGITLGRSLVEKDKVTVAAEILKGAEARKVRLLLPEDHVAALSMD
                     DTETQVFGNNDFPAEMIRFDIGPKTVKRYAAEVAKARTIFWNGPMGVFEKAPFAKGSV
                     ALAEAVGNSKAHTVTGGGDSVSMMNKAGIASKIGHISTGGGASLEFIQDKTLPGVEAL
                     KE"
     gene            8754..>9212
                     /gene="pyrG"
                     /locus_tag="PKH10_02505"
     CDS             8754..>9212
                     /gene="pyrG"
                     /locus_tag="PKH10_02505"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957317.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HNT27028.1"
                     /translation="MKQTKYIFFTGGVISSLGKGIASTTVGALLEARGITVNLLKFDP
                     YINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFCNITVGRNNNFTTGQIYDTVIT
                     KERKGEYLGKTVQVIPHITDEIKSKIRQLAPTADIIQVEIGGTVGDIESLP"
BASE COUNT         2119 a         2727 c         2732 g         1634 t
ORIGIN      
        1 gcatcgacaa gaaaggcaag aacatgaaga tagccgtcta cgatctcggc ggcggcacct
       61 tcgatatctc gatactcgaa ctgggcgacg gcgtgttcga ggtgctcgcc accaacggcg
      121 acaccttcct cggcggcgac gatttcgacc agcgcatcat cgaatggctc gtggaccagt
      181 tccgcaagga gtcgggcatc gacctgtcca aggacgccat ggcgctccag cggctcaagg
      241 acgcggccga gcgggcgaag cacgaactgt cgagcgcgat ggagacgacc atcaacctgc
      301 ccttcatcac cgccgaccag accggtccga agcatctcaa catcacgatg agccgcgcga
      361 aactcgagaa tatcgtcgag gatctcgcga aagggacgct cggcccctgc gagaaggtgc
      421 tcaaggacgc caaggtctcg gcctccgaca tcaacgaggt catccttgtc ggtggcatga
      481 cccgtatgcc gatgatacag cggctcgtca aggagttctt cggccgcgag ccgcacaagg
      541 gggtcaaccc cgacgaggtc gtcgcgatcg gcgcggcgat acagggcggc gtcctgaagg
      601 gcgatgtgaa ggatgtgctc ctgcttgatg tcaccccgct ctcgctcggc atcgaaacgc
      661 tcggcggcgt cttcacgcgg ctcatcccgc gcaacaccac tatcccgacc aagaagaccg
      721 aggtgttctc caccgcggtg gataatcagc cggcggtcac cgttcgcgtc tttcagggag
      781 aacgcgagat agcggtcgag aacaaactgc tcggcgagtt tgacctgcag gatatccccc
      841 cggcgccccg cggcatgccg aagatagagg tgtcgttcga catcgacgcg aacggcatac
      901 tcaatgtcag cgcccgcgac atcggcaccg gcaagcagaa gaacatcgtc atcaaggcgg
      961 gctccggcct gtcggacgac gagatcaaac ggatggtcag cgaggcggag aagttcaagg
     1021 cggaggacga gaagcgcaag acgctcgtcg aggcgcgcaa caacgccgaa caactcatct
     1081 actccaccga gaagtcgttc aaggagtacg gcgacaaact ctccgacgag gagaaacgcg
     1141 atgtcgagga gaagatagcc gacctgcgcg gcgtgctcga caaggacgat ctcgaggtga
     1201 tcaagggcaa gacgcaggcg ctcggccaga gcgcctacaa gatcgccgaa aaggtctacc
     1261 gcggcgccgg caccgaacag gggaaagacc ccggcgccga accgtcggac gacgacaagg
     1321 gcgacgacaa agtggtcgac gccgattacg aaccgaagaa atagacgggg ccgctgaccc
     1381 atggccgacc tgtacggcat actcggggtg cggcgcgacg ccacgctcga cgagataaaa
     1441 aaggcctacc ggcaactggc catcaaatac catcccgacc gcaacaaggg cgacaaggcg
     1501 gccgaggaga agttcaagga gataaatcag gcctacgaag tgctctccga cgccgaaaag
     1561 cggaagatgt acgaccagtt cggcgaggac gggataaagg ggtcgccctt ctcggccggc
     1621 gccgggggct ttgacttcag cgacctgttc agcggcaact tcggcggcgg caacgcgggc
     1681 ggcgggctcg gcgacatatt cgacgagttc ttcggcggcg gcgcgcggcg acgcggtcgg
     1741 acggccgcgc ggcagggaag cgacctgctc gtcaccgtga cgctctcgtt cgatgaagcg
     1801 tatctcggga cgcgtcgcga tgtcaaggtc gcccggagcg gtcggtgcgc gaaatgcgcc
     1861 gggtccggtg ccgaaccggg cgcaagccgc aagacctgcc ccacctgtca ggggcgcggt
     1921 gaggtgcgtg tgtcgcacgg ctttttccag atggcgcaga cctgcccgag ttgtcagggg
     1981 caggggagca tcatcgagaa accatgcaag gcgtgtcagg ggaccgggta tgttcgcgag
     2041 gaacgcgaac tgtcggtgag catccctgcc ggcatcgaca ccggacagaa gatccgcatg
     2101 gcgagcgagg ggaacgccgg cgcgaacggc gggccgcgcg gtgatgtcta tctgaatgtg
     2161 caggtgaagg accatccgct ctttcaccgc gacggcaacg acatctacct cgaactgccg
     2221 gtatcctacg cgcaactgac actcggcgat acggtcgagg tcccgacgat ggaggggaag
     2281 gtcgatctca aggtgccgtc cggtacgcaa cccgatacca agatgaggct ccgcgagaag
     2341 gggttcccgg cggtgcaggg gcgcgggcgc gggaaccagt tcgtcgtcct ccgcctcgag
     2401 gtgccccgca acatctcgtc gaaacaccgg aagatcgtcg aggaactgaa aggattcgac
     2461 ggcgaactta aggagcggcc gttcctcaag gatttcgccg aaaaggtaaa aaggaccttc
     2521 tctctataat cgttcttttc gtatactatt atttgacatc cgatcctttt ttcgctatag
     2581 tgcccccatc tgggtgctgg ggggcagggg ctttccagca ggtcgatcgg ggatggtcgg
     2641 gccgctgtcc cccatatcca aagaggggta ttgcatgagg agtaagagtt tagtgatcgg
     2701 cgtgttgttc ttcttcctgc tctgtgtcgg ctgcaccgag gaaaaaccgg cggtcgatgt
     2761 gaactcgttc gcgaccgact gtctcaagaa ctgcgcgatg gccttcaacg ggtgcatgaa
     2821 actctccaag tcgcccgagg cgagtaagca gtgcgaggtc ttcttcgaca aatgcaagaa
     2881 cgactgctcg gctaaagaga agccggcgcc cgtcgccgcc ccggcgcccg ccgccgaacc
     2941 cgccgccgcc gccgccccgg cgcctgccgc cgctcctgct ccggccgccg ctccggcgcc
     3001 cgctcccgcc ccggcgcctg ctccggaagc gaaacagtag tcgcgacgcg cgacccgctt
     3061 ataaaatttt gctttttact ccggcgacgg gtatagtccg cccgttcgtt atccatgctc
     3121 gtcttcggag gacctcatga gcgtcaccat tgagaaactg agcgacatcg agatccagaa
     3181 acggggcatc cgccagtggc ccgtctggga aaaagagata tcgaccttcc cgtggttcta
     3241 cgactcgcag gaacagtgcc tgttcctcga cggagaagtg gtcgtgaccg acagtgacag
     3301 cgggaagaaa taccatatcc ggcgcggtga tttcgtgacc tttcccgccg agatgtcctg
     3361 tacctgggag gtcaggcaac cgataaagaa acactaccgt ttcgagtagc gcgcgatggg
     3421 cagggaccga ctcatgatag cggcgctcga cgagggccgc ctcatcatcc ccgatcaccg
     3481 gtgcttcttt ctcgttcgcc gcgccgcggt gcggaccggc aagaacagtt cgcgctacat
     3541 cgatctcgaa ctcaccgacg gcgtatcgac cgtcaacggc aaggtatggg aggtcaccga
     3601 cgatatcgag cgggcgaccg agacgggaag catcatcaag gtgacgaacg gtcgcgtggc
     3661 gaaatatcag aattccctgc aggtcatcat cgaggacgcc ctgccggtgt cgcccgagga
     3721 acttgacgcc ctgccgggca tcatacccga atccaaactg gggccggcgg aactcatggc
     3781 gcggtggcgc gcgcttgccg acgggctcga tgcgggacgg cgcgccgtta tcaccgagtt
     3841 cgaggcgtcg ggtagagtgt gggacctttt ccgctcgata ccggcgggca agagcatgca
     3901 ccataactat cgccgtggac tgtgggaaca ttcgctgcag gtgaccgaac tggcccgtga
     3961 catcgcggcc cgctacgaga agcgctaccc ggtgagcgtc ccgaacatca tccttggtgg
     4021 cctgctccac gatgtcggca aggtgttcga gttccaagtg aacgcctcga cctccatcgt
     4081 cgagaaatac agcgaccgcg ggcggctcct cggccatgtc tacatgggcg cctcgttcat
     4141 cgagaagata ctcaaaaaag cgctgcccga cgatgccgac ctgcagatgg aactcctgca
     4201 catcatgctc tcgcaccacg gcgagtacga gttcgggtcg cccaagaagc cgaagaccat
     4261 ggacgcgctc atcatcgcgc tcgccgacaa tctcgacgcg aacctcgacg cggtgaacat
     4321 cggattcaac ggcgacatgg aggatcggtg gaccaagcag atattctcgc tccagcgccc
     4381 gttctaccgg acggcgacga cggtatcgga ggataacgaa tagtcttttc ttaagcaaaa
     4441 ggaggcatca caatggctca gaagatcaga gtggctatca acgggttcgg tcgcatcggt
     4501 cgcatggtgt tccgcatcgg gttcggccgc gccgacatgg agttcgtcac gatcaacgac
     4561 ctcaccgacg cgaagacgct cgcccacctg ctcaagtacg attcggtcgc gggtatctgg
     4621 ccgcacgagg tgaaggcgac cgagaccgcc atcgtcgtcg acgggaagga gatcccggtc
     4681 accgcgataa aggacggcgc ccaactcaac tacaaggata tcgatgtggt cgtcgagtcg
     4741 accggcaagt acgccaaaaa ggaaaaggcc gagatactcc gcgcggcggg cgccaagaaa
     4801 gtgctcatca gcgcgccggc gaacgattcc gacatcacgc tcgtgctcgg cgtcaacgat
     4861 aaggcctacg acccggcgaa acaccatgtc atctcgaacg cttcctgcac caccaactgc
     4921 tatgcgccgg tcacctacat cgtccacaaa tactggaaga taaagaaagg gttcatgacc
     4981 accatccact cctacaccaa cgaccagcgc atactcgacc ttgagcacaa ggacctgcgc
     5041 cgcgcccgcg ccggcgccct gtcgatgata cccacgacca ccggcgccgc caaggcggtc
     5101 gggctcgtca tccccgagct caaggggaaa ttcgacggca tctctatccg cgtcccgacg
     5161 cccaatgtgt cgctggtcga cgcggtctac gaactcgaat cggaagcgac caaggaagcg
     5221 gtcaacgcga agttccgcga atatgcgaac ggtccgctga agggcattct cgccgtttcc
     5281 gacgaggaac tggtatcgaa ggacttcaac ggcaacccgc acagcggcat cgtcgatgct
     5341 ccctacacgc aggtcatggg cggtaacctg ctcaaggtcc tcgcgtggta cgacaacgaa
     5401 tggggctatt cgaaccgcat gatcgatgtc atcaccaaag tgcttatgaa gtaaggtcgt
     5461 tcttccccgt tccttttttc acacaaaaag tgtcttggcc cgattttcag gacacctctt
     5521 gcaaaaaacg cttgacctgt taagatgaag cgatcgatga tgggagagaa ctcgtgacgg
     5581 cactcaaaaa actcatggtc ccgaaaaaaa agatcgttga tctctgcaag agatggcaga
     5641 tccgggaatt ctcctttttc ggttcgatcg tcggggattc cttcacggaa gcgagcgatg
     5701 tcgatgtgtt gattcgtttc gaccggtcga cgcacctcgg ccttttcgag atcgaggcga
     5761 tacgcgagga gttggaggcg cttttggggc gaccggtgga catcgtggag atggacgcgg
     5821 tgaaaaatcc ctatcgccgt gcggcgatac tggcctcgca cgaggtgctg tatgccgcat
     5881 aaggaccgcg atcttgccta tagccacatc ccggaactca tttcccggat agagccgctt
     5941 ctcccgaaaa agacgacatg aaacggtcgg tcatccggct actgcttgtt gccgtgttcc
     6001 ttttttccgc ctgcgtcccc tctaagaaca caccgaacga cgacgatccc tgcgccggtc
     6061 gctgcgggat ggtgaacggg gttgcctgtg ggtcctgttc gtacggtctt atctgcaaca
     6121 cggagaatta ttgtgtcgag atggcgtatg acgacattcc tgtggaggaa tggcccgaca
     6181 tcgaccaatt ctcatgtgcc gatatgaaat actatgccaa tgtcaaagcg gccggatttc
     6241 ctctcaagga cacgaaggga aagataacct tctgccgccc cggttgcgat accccgaccg
     6301 agaatgaccc gcaatgcgta aaaaatctct ggacatggat caactggaaa cggtaccagg
     6361 aatataccac aggggccgac accctgcgcg aatgttatcc atggccttgt gaattgcccg
     6421 acatgaaggc ccgcaccaac ctccacgaat gtgacaggaa tcttgcgagt caagaatata
     6481 tagccgatat aggggctcta tacgatctca aaatatacaa aagcaccgta ggcattctaa
     6541 tgaccaatcc ccatgcaagc aaggcgatgg cctacgatgt gacaaaggat gcctatacca
     6601 gtatcgcgca cagcggtcac ggtgtcggtt accattatga ccggttcgtc ttctacacaa
     6661 catatgaaga ttatgtgaat tccgataccg gctacgggaa cacctacatc gttacagcca
     6721 agagaaacgg ggccgattac aaatacgaag tgatctatga cgatgctgaa catatgtcgc
     6781 agttcaatcg ccctcctttt gtgggggaga aatgggttct gttgaatgtt gatcatacgg
     6841 gacagggcaa gatagaagtc ctttatgcca aggtggatga atggaaatgg catcccctga
     6901 aactggggca ggcatatgaa ggaaacatcg tcgacaaccg cctgaccttc ataaccgatc
     6961 agcgccagat cttcgtctgc gaccttgaca aactaccgtt cgacccggcc accgaatgcc
     7021 tgcgcataga ccgcgctgga gagacggcct atcaaccgcg attgaacgag gagaataaac
     7081 cccagttggt ctacttcaac gccgcaggcg acaacaacat gacctatgtg gatctgtcgg
     7141 gagaaaaacc ggcgtattcc tcgttgccgg tcactatatc cgaacctcaa agtacaggag
     7201 cctatcctca tcaatttaaa ggggacttaa ttctatacga tgaagcctat atgccgcttg
     7261 acaattcgcg attggactac aaagcttgct tctaccgcgt tgacacgaaa aagaattatt
     7321 gtccgatgaa gtatacggac ccattcttga atcgctatga tatggggttc aattcttttg
     7381 acggtaacta tcaactttgg aaaacgcctg ccgggacaat agctgtcgta agagatatcg
     7441 cttgttattg tgagaaagaa ggcgtctgcc cgtttgagga gtagcgtccc ccctaaaaat
     7501 tgactctttt tccccgccgc gctatagtcg cgcgaccacc gaacgccgtg tcaggaggaa
     7561 caccatggcc atcaagtcta tcagggacct cgcgatccgc gagaaacggg tattcatccg
     7621 caccgatttc aatgtgccga tggacaaaca ggggaacatc accgacacca cccgcatcga
     7681 ggcggccatc ccgacgatcc gttacgccgt cgagcataac gcgcgggtca tcgtcgcgtc
     7741 gcacttcggc cgtcccgacg gccagcgcaa gccggaatac accatggaac cggtcgcggc
     7801 gaaactcgcc gaactgatgg ggatcgaggt gaccttcttc gaggactgca tcggcatggg
     7861 cccccagcag atggtccgcg agataaagcc gggacaggtg ctcgtgctcg agaacctccg
     7921 tttctacccc ggcgagggcg acaatgacgg cgccttcgcg aaggatctcg cgaaactgtg
     7981 cgatgtctac atcaacgacg ccttcggggt gtcgcatcgc aagaacgcgt cggtccacgc
     8041 cctgccgctc ctcatggaga ccaaggggat gggcttcctg atggagaaag agataacgga
     8101 actcgggaaa ctggtcaact tcaagcacgg cgacggtttc gcggcgatac tcggcggcgc
     8161 gaaggtgagc gacaagatcg gcatcatccg gtcgctcctc gattcggccg acgcgctcct
     8221 catcggcggc gcgatggcct gcaccttcct cgcggcgaaa ggcatcacgc tcgggagatc
     8281 gcttgtggag aaggacaagg taacggtcgc ggccgagata ctcaaagggg ccgaggcgcg
     8341 caaggtccgg ttgctcctgc ccgaggacca tgtcgccgcc ctctccatgg acgacaccga
     8401 gacgcaggtg ttcggcaaca acgatttccc ggccgagatg ataaggttcg acatcgggcc
     8461 caagacggtg aagagatacg ccgccgaggt ggcgaaggcg agaacgatat tctggaacgg
     8521 cccgatgggg gtgttcgaga aggcgccgtt cgcaaagggg agcgtggcgc tcgccgaggc
     8581 ggtcggcaac tcgaaggccc ataccgtcac cggcggcggc gattcggtct cgatgatgaa
     8641 caaggcgggc atcgcgtcga agatcggcca catatcgacc ggcggcggcg cgagtttgga
     8701 gttcattcag gacaagaccc tgcccggcgt ggaggcgctc aaggaataac cccatgaaac
     8761 agaccaagta catcttcttc accggcggag tcatcagttc gctcgggaag gggatcgcct
     8821 cgaccaccgt cggcgcgctc ctcgaggcgc gcggcatcac ggtgaacctg ctcaagttcg
     8881 acccgtacat caatgtcgat cccggcacca tgagcccgtt ccagcacggc gaggtgttcg
     8941 tgaccgacga cggcgccgag accgatctcg acctcggcca ctacgagcgg ttctgcaaca
     9001 tcaccgtcgg ccgcaacaac aacttcacga ccggtcagat ctacgacacc gtcatcacca
     9061 aggagcgcaa gggcgaatac ctcggcaaaa cggtgcaggt gatcccgcac atcaccgacg
     9121 agataaagag caagatacgg caacttgccc cgaccgccga catcattcag gtcgagatcg
     9181 gcggtacggt cggcgacatc gagagcctgc cg
//
LOCUS       DAOQUE010000047         9077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8517_length_9077_cov_6.392707, whole genome shotgun sequence.
ACCESSION   DAOQUE010000047 DAOQUE010000000
VERSION     DAOQUE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8517_length_9077_cov_6.392707"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3093
                     /gene="mobF"
                     /locus_tag="PKH10_02510"
     CDS             <1..3093
                     /gene="mobF"
                     /locus_tag="PKH10_02510"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HNT27029.1"
                     /translation="GGQDLTFSAPKSVSVLAFCSEDPAVRRDMLQAHREAQRETIQWL
                     EKECAAVNIYKDRKISGTEKTGNLIVSSFTHDSSRPVRLPDGSHQIDPQLHTHAIVHN
                     MTKDRTGKWRAIAFRDFYTSKMLAGAIYHNALEKGLRDRGYETLRNNDTTFEVKGVPA
                     ALLERHSQRRTAIEAYAKKNGIDLSDPKQMAKVTKNTRVSKKEVDSDRLHASWRESGR
                     AYRLTREALASDRPPGPAVSSQAILRQATDHVSERKAVLTDRDILLESLRQNGGRHAT
                     TDLEAGIRKMKERGELIHTSDGRLATKDQLEAEHHLLTAVRAGKGAGTPLMKPEQVDF
                     SEYQRERGFELSAGQKSASEELLTTADRYIAIQGYAGAGKTTFLQHSAEQLEKRGACT
                     VRGFAPSAAAAAVLQKETGIKSQTLDSFLQEAKKPMPGSPGVAMRSREPGHREVWIID
                     ESSMLSSRKAAELVTAADRMNAQVVFLGDTKQLSAIEAGNPFEQMQKHGIRTVEVTEI
                     NRQQLSVRDAAKMEKAGYSPEEIAERRAKAGKYQEAVYDVYRGAMRTALEKVDVVELR
                     DARERGETLVKDYFKYRDADKHDPSKAKDTLIGTSRNDRIAELNGHIRDEMKNRGELQ
                     GDERRYTTLKSKGLTDAEARQPKNYRNGDIVQFRAADKDNGIEAGAYGTVTGRDERTG
                     TITVRLKTGRDLAVPLTVKEVHTRTMTPESKRDAAAYREGETVTFNRNYPSIGIKRGD
                     SFTIQTVQGDTVHLADKEGRLKEWTPARGPFGGYDLTVGQEKIRGPALSLYYKEAISL
                     QEGDRIRFTENIGHTEKTRTGQVKTREFVNGISAKVLEVGEGTVRIEYEGHTRTIDLS
                     NEKYQHLKYGYAATFHDLQGQTGNRILMEADTQKDKALLGEKTGLVGLTRGQHEAIVY
                     TDDRENLPGALGKKQGQEYAVKSEPERTPVTTALRHTVPPPDFKNMTADMRELATRKN
                     GASLHTADMRSLERPEYRLSVKDNARELEKGETPEKAKVPEQQKTPVKQREREISHDR
                     "
     gene            3162..3716
                     /locus_tag="PKH10_02515"
     CDS             3162..3716
                     /locus_tag="PKH10_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27030.1"
                     /translation="MADDRDLLLEEEDLPSPAPTPAPTSARNPQHADPVHDAKREELA
                     KRERQIQDLLSTIAAERNRDVKSLSNILDSNYKTSQQKQDQLLAAIETLRKRDILWVR
                     VGKGLGVVLALLFVLQAVVWLAAPKVAPATDKSIYDGLRIDTTSRPGEVWVYSENNSW
                     IKDMEQKVDDEKLRNGWCKALIKK"
     gene            complement(3896..4738)
                     /locus_tag="PKH10_02520"
     CDS             complement(3896..4738)
                     /locus_tag="PKH10_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27031.1"
                     /translation="MNTILPLSFSDFNLPGAEKVLAPLFNMAVQHRIELMCIGAAALP
                     IALRQQGATPRGRFTQDMDVSFAIRGWDEYFAFIESLLGLGLFHRAKETHRLYGPEKT
                     IVDLVPFGGVAGSRSSIKWPPENDTEMSVLGFDPVYTVSPEYLVDGRFTVRIPRPEGF
                     LVLKLFAWADRKERTRKDAEDIGEILYSCCYGEELYTRYAHLIDRPDFDFMACCAEIL
                     GIKIRDSVPTLTLPLAEILKQELAQETSSFIATLAGSLSYGESQRLTRELLRGLVGAD
                     SVSL"
     gene            complement(4707..5732)
                     /locus_tag="PKH10_02525"
     CDS             complement(4707..5732)
                     /locus_tag="PKH10_02525"
                     /inference="COORDINATES: protein motif:HMM:NF021469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HNT27032.1"
                     /translation="MKADKDHSQLLRKVATVLKQEHGILLTVKNAGQQPQATLLIKGR
                     KFILPLIIRPTFAGNAAFFALMERRRELGNKPLLLITERLSPVMLERLKKVEIYAADA
                     IGDLYVEGKDLYLWASGKKPTKESRTMGNRGRAFLPAGLKVLFALLTDKKLLAADYRT
                     IAQRTGVSLGAIGPILNDLATGHFLVQDDTGRRLMNTAMLTERWITAFAERLRPTLLM
                     GTYRPLKEPLNLKTLAFSKDIFLGNEAAAEHGEHLLKAGTITLYSKQDPIEIVKQLRL
                     IPDPQGTVELLKPFWDMDWFAETPEYQKGIVPYFLIYADLIASGYDRNFTAARSIHEH
                     YLAALVQ"
     gene            6795..>9077
                     /locus_tag="PKH10_02530"
     CDS             6795..>9077
                     /locus_tag="PKH10_02530"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNT27033.1"
                     /translation="MFSKAHAKHQRGSALISKLMAVSMTIVLSTGFIGSAGCSKTEKD
                     DETQKARLLSENIGSAADDYGYNVVVDNMGNVYLTGATQGILTDPNNLHDSLERILLG
                     DISGSRSEEDEDYDAYLIKYNADGSRAWIRQWGTVGDEGGYSLSIDPFGDIYVLGMTT
                     DDLWEENTGAWDLFVEKYSPAGERKWGMQWGSDKQDTGEGIATDINGAVFVVGNTLGV
                     FGEDDLSRNDGFITKISNTGVVQWTKQWNSTEDAAETAHGIALYGDYAFVVGDTTGTL
                     QTGNKIGGRDCTLMKFDKNAVSAPDWVWVKQWGTASDDFLYGIAMDRVGNIHVSGITA
                     GTIGAANIGGKDMFVQKYPANMTASSSATWTRQLGSTANENVSSVTVDIAGVVYVAGT
                     TLGNYDGNTNSGGSTDFFFTKFSSSGTKQWSHQKGVGGGLVMYTRSTGIDPAGRLYIT
                     GHTGGTQVGSGLYFNYDVFLYKYVDNQKNWVFTREFGTTTEDKAAASATDGSGNTYVT
                     GYTFGALQGTNLGGNDAFLIRYNSDGSKAWTRQWGSSALEGGAAIAIDADRNIVVAGV
                     TAGSLTDQHGQSLPYSGSYDLFVAKFSPDGSLLWMKEWGTTAEEWVYDVHVGKNGKIY
                     LTGYVGPSESRDGFITALSDHGSSAVVEWSQIWGTASYDTGYSVTTDSNNNIFVSGRT
                     AGTMSGCTSAGGTDNVLLKYDVAGTEQWHRQWGSSGTDHAYAVALDKLETALYVSGIT
                     TGVLGSANLGNSDAFLQKLDL"
BASE COUNT         2365 a         2387 c         2553 g         1772 t
ORIGIN      
        1 gggggacagg acctgacctt ttccgcaccg aaaagcgtca gtgtcctcgc cttctgcagt
       61 gaagatcccg cggtcaggcg ggacatgttg caggcgcacc gggaagcgca acgcgaaacg
      121 atccagtggc tggaaaaaga atgcgccgcc gtcaatatat ataaggacag gaagatctcc
      181 ggcaccgaaa agaccggtaa cctcatcgtc tccagtttca cccatgactc ctcccgcccc
      241 gtcaggctcc ccgacggctc ccatcagatc gatccgcagt tacataccca tgccatcgtc
      301 cataacatga ccaaagaccg taccgggaaa tggcgcgcca tcgccttccg cgacttttac
      361 acgagcaaga tgctcgccgg cgcgatctac cataacgccc tcgaaaaagg tcttcgcgac
      421 agaggctatg aaactctccg gaacaacgat accaccttcg aggtgaaagg ggtcccggcc
      481 gccttactcg aacggcattc ccaacgccgg accgccatag aggcctatgc gaagaagaac
      541 gggatagacc tctccgatcc gaaacagatg gcgaaagtga cgaagaacac ccgcgtcagc
      601 aagaaagagg tcgatagcga ccggctgcac gcttcctggc gcgaaagcgg ccgcgcatac
      661 cgcctgaccc gcgaagcgct cgccagcgat cgtccccccg gtccggcggt atcgagtcag
      721 gctatcctgc ggcaggcgac cgaccatgtt tccgagcgga aagcggtcct taccgaccgc
      781 gatatccttc tggagagcct tcggcagaac ggggggcgac atgccactac ggacctcgaa
      841 gccggcatca ggaagatgaa agagcgggga gaactgatcc acacgagcga tggccgcctc
      901 gccaccaaag accagttaga ggctgaacac cacctactca cggcggtccg cgccggcaaa
      961 ggggcaggaa ctccgctcat gaaaccggag caggtcgatt tcagcgagta ccagcgggaa
     1021 cggggctttg aactctccgc cggacagaag tccgccagcg aagaactcct taccaccgcg
     1081 gaccgctaca tagcgatcca agggtacgcg ggtgcgggga agaccacctt cctgcaacac
     1141 agcgccgaac agttggaaaa gcggggcgcc tgcacggtcc ggggctttgc gccgagcgcg
     1201 gccgcggccg ccgtactaca gaaggaaacc ggtataaaga gccagaccct cgactcgttt
     1261 cttcaggagg cgaagaaacc gatgccgggt tcccccggcg tagccatgcg gagccgggaa
     1321 ccgggccacc gggaagtatg gatcatcgac gaaagttcga tgctttcctc ccggaaagcg
     1381 gccgaactgg tcaccgccgc cgaccgcatg aacgcgcagg tcgtctttct gggcgacacc
     1441 aaacaactct ccgcgatcga agcggggaac cccttcgagc agatgcagaa acacggtatc
     1501 cgcacggtgg aagtcaccga gataaaccgt cagcaactct cggtacggga cgccgccaag
     1561 atggaaaaag cgggctattc cccggaagag atcgccgagc ggcgagcgaa agccgggaaa
     1621 tatcaggaag cggtctacga tgtttaccga ggcgccatga gaacggcgct cgagaaagtc
     1681 gatgtcgtgg aactccgcga cgcgcgggaa cgcggggaaa cactggtaaa agactacttc
     1741 aaataccgcg atgccgacaa gcacgatccc tccaaagcga aagacaccct catcggcacg
     1801 agccgcaacg atcgcatagc ggaactgaac ggccatatcc gcgacgagat gaagaaccgc
     1861 ggcgaactgc agggcgacga gcggcggtac accaccctga aaagcaaggg actgaccgac
     1921 gcggaagccc gtcaacccaa gaattaccgg aacggcgaca tcgtacagtt ccgggcggcc
     1981 gacaaagaca acggcatcga ggcgggggcc tacgggaccg tcaccggtcg cgacgagcgg
     2041 accgggacca taacggtgcg actcaagacc ggccgggacc tcgcggttcc cctgaccgta
     2101 aaggaggttc atacccgcac catgaccccg gaaagtaagc gggacgccgc cgcataccgg
     2161 gaaggggaga cggtaacctt caaccgcaac tatccgagta tcggcatcaa gcgcggcgac
     2221 tccttcacga tacagaccgt acagggagat actgtacatc tggcagataa agaaggccgc
     2281 ctgaaagagt ggaccccggc gcgcggcccg ttcggcggct acgatctcac cgtgggacag
     2341 gaaaagatac ggggccccgc cctgtcgctc tattacaagg aagctatctc cttacaggaa
     2401 ggagaccgca tccgtttcac cgagaacatc ggccataccg aaaagacccg caccggacag
     2461 gtaaagaccc gcgaatttgt gaacggcatc agcgccaagg tcctcgaagt aggggaaggg
     2521 acggtacgga tcgagtacga aggccatacc cgaaccatcg acctctcgaa cgaaaaatac
     2581 caacacctca aatacggcta tgccgccacc ttccacgatc tgcaggggca gaccggcaac
     2641 cgcatcctca tggaggccga cactcagaaa gacaaggcgc tcctcggcga gaagaccgga
     2701 ctggtcggtc tcacccgcgg ccagcacgaa gcgatcgtct ataccgacga ccgggagaac
     2761 ctccccggag ccctcggcaa gaaacaggga caggaatacg ccgtcaagtc cgaaccggaa
     2821 cggaccccgg taacgacggc ccttcggcat accgtgccgc cgcccgactt caagaacatg
     2881 accgccgaca tgcgggaatt ggcgacgcgc aagaacggcg cttcactcca taccgccgat
     2941 atgcggtccc tcgaacgccc ggagtaccgg ctctccgtca aggacaacgc gcgtgaactg
     3001 gaaaagggcg aaacacccga aaaagcgaag gttccggaac aacagaaaac gccggtcaaa
     3061 cagcgtgaac gcgaaatatc gcatgaccga tgaaattctt gaccttatac caccgggccg
     3121 gtaagttgcg ctgttttttg tgaactgaac ggagacccgc catggccgac gaccgcgatc
     3181 tgttgcttga agaggaagac ctgccttctc ccgctcccac tcccgctcct acttccgcca
     3241 gaaatcctca acacgccgac ccggttcatg atgcaaagag ggaagaattg gcgaagaggg
     3301 agaggcagat acaagacctg ctatcaacca tagccgccga gaggaatcgg gatgtaaaat
     3361 ccctctcgaa catcttggac agtaattata agacaagcca acaaaagcag gaccaactcc
     3421 ttgcagctat cgaaacgctg cgaaagaggg acattctttg ggtgagagta ggcaaagggc
     3481 tcggggtcgt gctggccctg ctgttcgttc ttcaggcggt cgtttggctg gccgccccga
     3541 aggtcgcccc ggcgactgat aaatcgatct atgatggttt gcggatcgac acaacaagtc
     3601 gccccggaga ggtatgggta tactcggaaa acaattcttg gatcaaagac atggagcaga
     3661 aggtcgacga cgagaagctc cgtaacggct ggtgcaaggc gctcatcaag aagtagggga
     3721 acaactcctt tttgacatcg ttggacccaa ctgcataacg gggcaaaatg tcaagagcag
     3781 tttttgctaa ctatctgtta gcacaagaaa agttcgattc ttgaacctcc agaaatcagg
     3841 gtagtttcaa cgccaatatc atgtcgcggt tgaggccgcc ggataatgat gtgtgtcaca
     3901 agctcacgga gtctgccccg acgagtccgc ggagcaactc tcgagtaagg cgctggctct
     3961 ccccgtatga aagcgacccg gcaagtgttg ctatgaaaga agatgtttct tgggccaatt
     4021 cctgcttgag tatttcggcc aacgggagcg tcaaggtcgg gacggagtcg cgaatcttga
     4081 tgccgagtat ttccgcgcaa caggccataa agtcaaaatc gggacggtct atcagatggg
     4141 catatcgtgt gtagagttcc tctccgtaac aacatgagta aagaatctcg ccgatatctt
     4201 ccgcgtcttt gcgcgttctt tctttccgat cggcccacgc aaaaagtttc aacaccaaga
     4261 agccttccgg ccggggaatg cgcacggtga atcggccatc gaccagatat tcgggagaga
     4321 cggtgtacac cgggtcaaag ccgagaaccg acatttccgt atcgttctcc ggcggccatt
     4381 tgatgctgga tcgagacccc gcgacaccac cgaaggggac cagatcgaca atggtttttt
     4441 ccggaccata caaccgatgt gtttctttcg cccgatgaaa cagaccaagc cccagaaggg
     4501 actcgatgaa ggcaaaatat tcgtcccatc cacgaatggc gaaggagaca tccatatcct
     4561 gcgtaaaacg accacgcggt gtcgcgcctt gttgcctaag cgcgatcggt aaggccgccg
     4621 cgccgatgca catcaattct atccggtgtt ggaccgccat gttgaataga ggagcgagaa
     4681 ccttctccgc cccggggaga ttgaagtcac tgaacgagag cggcaagata gtgttcatga
     4741 atactccgtg cagccgtgaa attgcgatca tatccgctgg cgatcagatc ggcatagatc
     4801 agaaaatagg ggactatgcc cttttgatat tccggcgttt ccgcaaacca atccatgtcc
     4861 cagaagggct tcagaagttc caccgttcct tgcgggtcgg gaatgagccg gagttgcttg
     4921 acgatctcga tcggatcctg tttcgagtag agcgtgatag tgcccgcttt cagaagatgc
     4981 tccccgtgtt ccgccgccgc ttcgtttcca aggaatatgt cttttgagaa cgccagcgtt
     5041 ttcaggttca gtggttcctt caaaggccgg tatgtcccca tgagaagtgt cggccgcaac
     5101 cgttccgcaa aagcggtgat ccagcgctcg gtgagcatgg cggtattcat caggcgtcgg
     5161 ccggtatcgt cctgtaccaa gaaatgaccg gtcgccagat cgttcaatat cggcccgatc
     5221 gcgccgagag agacaccggt ccgttgtgcg atggtacgat aatcggcggc cagcaacttt
     5281 ttgtcggtca gtagggcgaa caacaccttc agccctgccg gcaaaaaagc tcttccccgg
     5341 tttcccatag ttcgggattc ttttgtcggc tttttgcccg aggcccagag ataaaggtcc
     5401 ttgccctcga cataaagatc gccgatggca tctgcggcat agatctcgac cttcttcaat
     5461 cgttccagca taaccggaga aagccgttcg gtaatgagca agagcggctt gttgcccaat
     5521 tcgcggcgtc tctccatgag agcaaagaaa gccgcatttc cggcaaaggt cggtcgaatg
     5581 atcagcggca gaatgaactt ccgccccttg atcaaaagag ttgcttgtgg ctgttgcccg
     5641 gcatttttaa ccgttagaag tatcccatgt tcctgcttga gtactgtcgc cactttccgt
     5701 agcaactggc tgtggtcttt gtctgccttc atgttgtgtt cactgctact gaacattaat
     5761 gaactatgaa cagcataccc ttttcgggcg ggttgtcaag aacaaaaatg agcccgactg
     5821 cacaaaaaga caaacagtga tgccgaagtc gggtagggga tcgagcctgt aagacggatt
     5881 cctttcagcc caagtttaca taaagcatct tatgcgaatg gacaagaggc aagagattcg
     5941 gatcctcgca atccccttga aaactgccgt aaaatcagcg agtaaaacac ttgcggctcc
     6001 taaccgtcta taatcattaa ggtcggagtg tcgctcaaga aaaaattccc gaaacctgca
     6061 aagtgctcaa taatacaggg atatattttc atgtcagatt attgtgaaga aaaaatagaa
     6121 ctcacgagtc ctttgaaaaa attaggacaa acgatgagtt gtatgcaaga agagcacttc
     6181 ggaagagcag atcggagaag cggttgaccg gaaataaaag ttgaaaagaa tatggagccg
     6241 tgcttcaatc ccctcataaa gacatagcaa aattccggct atcggggata tagcttggtg
     6301 cggagtagtg tctttcatat cgggaggggc cgcgaaacaa cggccattct tctttcccgc
     6361 acgccccatg atccttcgga caaaaacata acttatggtg ggagaaagtc atgagtcgtc
     6421 cagtcgctat gctgtttttc gtcgtgtgcg ggtatctgtc gctcctttcg gcggccgaac
     6481 cgtgtccggc ggggtggacc tttaacgccg ccaacaatca ctgttacaac ctcgaagcga
     6541 ccgggtattt ttgcgcggaa gggagaggat gtcggtgtat actccttgat gcccacaaaa
     6601 aaagttcaaa aaaagagaat ccttttcgag tgagccggta tcatatggac atcggatgaa
     6661 cacgctcttt gttttgccta agagagttga aggtgagaat aatgtgtgaa ccagacatgt
     6721 tagagagaaa ttgaaagaga taaaaaaacg cgaaaaagta tttagtggtc gggaaaaata
     6781 aaaagaggtg agttatgttc agtaaagcgc acgcaaagca tcaaagaggt tccgccttga
     6841 tctcgaagct catggcggtt tctatgacta ttgttctttc gacagggttt atcggatcag
     6901 cgggttgctc gaaaacagaa aaggatgatg aaacacaaaa agcccggctt ctttccgaga
     6961 atatcggttc ggcggcggac gattatggtt ataatgttgt ggtcgacaat atggggaatg
     7021 tttacctcac aggtgctacc cagggaattt tgacggatcc caataatctc catgattctc
     7081 ttgaacgtat tctcctaggc gatatttccg gaagcaggtc tgaagaggac gaagattatg
     7141 atgcttattt gatcaagtac aacgccgacg gttcccgggc atggatcagg caatggggta
     7201 ctgtcggtga cgagggaggc tactccctat ctatagatcc ctttggggac atctatgttc
     7261 tcggcatgac aacggacgat ctgtgggaag aaaacaccgg tgcttgggat ctcttcgtcg
     7321 aaaaatattc gccggcgggc gagagaaaat ggggaatgca gtgggggagc gacaaacaag
     7381 acacgggaga agggatcgct acggatataa atggggccgt gtttgtcgtt ggcaacaccc
     7441 taggggtttt tggagaggat gacctatctc gcaacgacgg gttcataacg aagatcagca
     7501 ataccggggt tgttcagtgg acgaaacaat ggaactccac cgaggatgct gccgaaacgg
     7561 cgcatggaat agccctttac ggcgattacg ccttcgtcgt cggagacacg accggaacgc
     7621 ttcaaaccgg gaacaaaata ggcggtagag actgcacact gatgaaattc gataaaaatg
     7681 ccgtcagtgc tcctgattgg gtatgggtaa agcagtgggg cactgcttcg gacgatttct
     7741 tgtacggcat agcgatggac agggtgggga acatccatgt ttcgggtatc actgccggca
     7801 ccataggcgc cgccaatatc ggcgggaagg atatgttcgt tcagaaatat cccgccaaca
     7861 tgacggcaag cagttccgcg acatggacaa gacagttagg gtcaacggcg aatgaaaatg
     7921 tgagtagtgt taccgtcgat atcgcaggcg tggtttatgt ggcaggtacc actttaggaa
     7981 attatgacgg gaacacgaat tcgggaggat ccacagattt ctttttcacc aagttttctt
     8041 ccagcggaac gaaacaatgg tctcatcaaa aaggggtcgg aggaggactc gtgatgtaca
     8101 cgaggtccac cggtatcgat ccggcaggaa gactttatat caccggacat acggggggaa
     8161 cgcaggttgg ttccggatta tatttcaact acgatgtttt cctttataag tatgtcgata
     8221 atcaaaaaaa ctgggtgttc acacgggagt tcggaacgac gacggaggac aaagcggccg
     8281 cttcggcaac ggacggcagt gggaacacct atgttacggg atataccttc ggcgctcttc
     8341 aaggtacgaa tctcggagga aacgatgctt ttctgatcag atataattcg gacggcagca
     8401 aagcatggac acgacagtgg ggtagcagcg cgctggaagg cggcgcggcg atagccatcg
     8461 acgcggatcg taacatcgtt gtagccggcg tgacggcggg ctctcttacc gaccaacacg
     8521 ggcaatcatt gccgtatagc gggagttacg atctttttgt cgccaagttt tcgcccgacg
     8581 gttctttgtt gtggatgaaa gaatggggca cgacggcgga agaatgggta tacgatgtac
     8641 atgtcggcaa gaacggaaag atatatctca ccggatatgt cggtccttcg gaaagtcgcg
     8701 atggttttat tacggccttg tccgatcatg gttcttccgc cgtggtcgaa tggagccaaa
     8761 tatggggaac ggcttcttac gacacgggct attccgttac cacggatagc aataacaata
     8821 tcttcgtgag cggcagaaca gcgggaacca tgagtggttg cacgagcgcc ggagggaccg
     8881 acaatgttct gttgaaatat gatgttgcgg gtaccgagca atggcatcgg cagtggggaa
     8941 gcagcgggac ggaccacgcc tatgccgtag cgctcgacaa actcgaaacg gcgctgtatg
     9001 tatcggggat cacgacagga gtcttgggga gcgccaatct cggcaattcg gatgcctttt
     9061 tacagaaact tgatctg
//
LOCUS       DAOQUE010000048         9073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8523_length_9073_cov_4.245065, whole genome shotgun sequence.
ACCESSION   DAOQUE010000048 DAOQUE010000000
VERSION     DAOQUE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8523_length_9073_cov_4.245065"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..2303
                     /gene="clpA"
                     /locus_tag="PKH10_02535"
     CDS             30..2303
                     /gene="clpA"
                     /locus_tag="PKH10_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HNT27034.1"
                     /translation="MKLDRKLNNIILGAFYEAKQGKHEFVTPEHVLYSLLFSQEGIEI
                     IEGVGGNIEVLRGEVKKFLETMVPRVVSGELLQSYSFQHLLELAAHRASSVGKKQVEV
                     TDVVVSLFDCEESHAAYYLTRQGIEQFDVMKFISHGLGALSEEVAHEQEQADREAARP
                     PRKKERDREKERDEGDPKGGIESYAVDLTALAEKKALEPLIGRADILTRTMQVLCRKT
                     KNNPIVVGEPGVGKTAISEGLAQRIVAGTVPAPLKDHRMFRIDMGSMLAGTKYRGDFE
                     ARMKKVLSSLAQREKVIIVIDEIHTIVGAGAVSGGSMDASNLLKPILTTGRLKCIGST
                     THEEYRKYFEKDRALSRRFQKIDLPETTIEETVDILKGIRSRYEEYHAVKYTDTALRA
                     AAELSARYVTERFLPDKAIDLVDEAGAFTRISGEERKTIEVSDIERVVALVARIPEKT
                     VSSGEGEKLQGLEAALRRQIFGQDGAVHAVVEAIKRSRAGFGRPERPVASFLFIGPTG
                     VGKTELARQLARELGVTLHRFDMSEYQEKHTVSRLVGAPPGYVGYEEGGLLTEAIRKT
                     PHAVLLLDEIEKAHADIYNTLLQIMDYATLTDNAGRKADFRNVIIIMTSNAGAREIGK
                     AVPGFGERALDGGELDRAVERIFSPEFRNRLDRVVRFERLDLDIITDIVKKQVAEFAE
                     QLREKKVEFTVTAAAYRHLAERGFSKEFGAREVGRLIQEEVKDFFVDEVLFGRLRNGG
                     KARAIFRGGRFSIEVVS"
     gene            2307..3002
                     /gene="aat"
                     /locus_tag="PKH10_02540"
     CDS             2307..3002
                     /gene="aat"
                     /locus_tag="PKH10_02540"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNT27035.1"
                     /translation="MVYQITRDRIAFPDPRLAEEDGLLAIGGDLSVDRLLAAYRQGIY
                     PWYTDEYPILWWSPDPRFVLFPEELRVSRSIAKLLRHNDFRVTVNRAFRQVVRSCAKV
                     PRRGQQGTWITADMIAAYQRLFEKGHAVSVESWLGDELAGGLYGVIIGRCFFGESMFA
                     WEPNASKIAFVKFVEFLRENGIRVIDCQMRTAHLERFGGRFIPLAHFLDILSGGCDEP
                     MPSWPVSLWSEKT"
     gene            3065..6658
                     /locus_tag="PKH10_02545"
     CDS             3065..6658
                     /locus_tag="PKH10_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27036.1"
                     /translation="MKWFTMVAVPAMFTVLFLSCEGTKTTTQDKDISLGDEDSLLADE
                     SVTDEEEPEGEETVKDDGPVAKDDGPVAKDDGPVVTDDAPVVTDDGTVEPDEDIVVTD
                     DIVTDDVVTDDVVTDDVVTDDVVTDDAVTDDIQPDDDQIETFTIGWCNLQYPADATAT
                     QGDNVTFYGRVYIAGLTDLTAEVDTNAQVIGQLSGNPSSGVDAATWPSDGWLDLLPNP
                     DNETAVAIGNNDEYMLEVPVMADPGVMDITVRFSGDGGATWTYCNANREGSEGYNGTD
                     VENPYDPAKHGHLTVEAAYDDPCDGDNPCEEPNRSVCTDANTDGVAECACDENYTLDG
                     GDTCINEKFVDCAANPSKPLNSHDVITQVKVNYTDLDGWGDPEICTTWDCNDGYHWNE
                     DEEACLETLTIGWCNTQWPTDASWYRGEEVAVYGRLYIAGLTDQSLDVDEAPQVIVQV
                     GSSPTGTSATDWMYWGAMVPNPDSKLAPGIGEDEDEYQFIGAVTETLGDWDHLVRVSG
                     DSGATWTYCNANRPGEGYNGTDSDNPYDLTKNGHFTVTSPCGEGFCDEPNKSVCADSD
                     SEPFYTCSCDTHYNDDDGNGTCEADRQLVDCMNALPENAVWDEDLPIYEGLGKLWQTW
                     NDGWKPEADTCPWVCAATYHEEDGDCVSDAPRTVNCIDNKPETSHWVADGAYNGDGTV
                     GQTWTGTEWIPPADECPWECDTDYHYENSSEQCVTNTIGRLCTNTLPTGADWVVLAPY
                     DGEGNLIQTWVGPGDDDWTPDTDTCPWECTEPGYHENTEVNQCLLTYAIGWCKIQWPP
                     TFEGIRGSSGETVYSRLWVDGVTDMTAGPDPLPQIKAQLGAGPRDTDPSTWGDLWMPA
                     VPNGEYSPSSPDYSPNNDEYMAMPVLDMPGGLYDYAYRFSGDSGQTWTYCDLNGSSDG
                     YTTDQAGRLAVYECDASEDCTYEAGKDICVTDDTDPMYRMCVECLVTDDCAEGNVCDT
                     ASYTCVECLDNTDCTDEVGLPVCDTANHNCVECVEDADCVDPLAYCTVDNACEVPPIG
                     KFADWPLTTDGSPTNVATGVLAGSYSGGTGIGLITYGVNGAYASNWTTALSPDADDYF
                     QITVTPDTGVSSIVITSINYSERRSSTGIRTYEIQYSIASDFSDPVVLDTVTVPDDDL
                     TRSHALTDLSIAVDQGETLYLRFFGYAAEASGGTWRIGSNALKLEGRIYPE"
     gene            6779..8683
                     /locus_tag="PKH10_02550"
     CDS             6779..8683
                     /locus_tag="PKH10_02550"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNT27037.1"
                     /translation="MRNLVLFSACVALFLVVGCAEKKKEPSGEIVKKMVTAAGGGTVS
                     WADGSASVDIPAGALAVDTEITIEKKDKKDFPETEKLYSAVWNFGPAGLTFSKPIRMT
                     IKTDKAKGAGPVAVVAVLRNDAWHSKGYDGIHAAGDPIMAGDPIMAGDPIMAGDPIMA
                     GDPIMAGVGHFSSFVVYVQAGDDILTDSDIVTEEGPVENEDLPENDADELLSDDDGPG
                     LCVENETKSEGGDYLVCHSGEWIIKRETRQWGTAEQDIAYGIARSSDDAVYVAGLTNG
                     DLEGNLSAGLSDPFLTKWSSGGVMEWTVQWGTAATDEARAVAVAEDGSIYVSGGTRGA
                     LDGNTLAGDYDIFLTKWTADGVKEWTRQWGTGAFDQGRAIGVDGAGNIYVTGETLGKL
                     DGNVSAGGYDAFLTKWSPDGTKQWTRQWGSATYDYPMGLTVGGDGSVYVAGRADNAMD
                     GEPHAGGRDPFLTKWSSDGAKQWTRQWGSANDDQASAVSVSGAVLYIAGYTAGTLSDQ
                     VSKGDRDFFVSAFTDTGEFLWTLQEGEETEDTATAITVDTAGEIWITGYTRIGVNDNF
                     LARANDQGEMLSFLQWGGPQVTLVDQANGLTISSLGEIFVTGQTDGKMDDSPATTGGE
                     EIFLTRLVVE"
     gene            complement(8661..>9073)
                     /locus_tag="PKH10_02555"
     CDS             complement(8661..>9073)
                     /locus_tag="PKH10_02555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT27038.1"
                     /translation="LPFPTDYRNIKVRAVGFLPAADLPTLYSTADILLFPSQAESFGL
                     TPLEAASCGAVPVLFGVGPLAENTIDGVTGVVVPPFDTDFFAAKIAALLNDVDARDRL
                     SKAGHEHIMKEFDPETVAKNYFELFGKITRPPVG"
BASE COUNT         1931 a         2513 c         2956 g         1673 t
ORIGIN      
        1 ctgtaccgtc gaaaaggcgt gaggtggcca tgaagctcga tcggaaactc aacaacatca
       61 ttctcggcgc cttctacgag gcgaagcagg ggaaacacga gttcgtgacg ccggagcatg
      121 tcctgtattc gcttctgttc tcgcaggaag gcatagagat catcgagggg gtcggcggca
      181 acatcgaggt gctgcgcggc gaggtcaaaa agttcctcga aacgatggtg ccgcgcgtcg
      241 tgtctggcga actgctccag tcctattcgt tccagcacct gctcgaactt gcggcccacc
      301 gcgcgagttc ggtgggtaag aagcaggtcg aggtgaccga tgtggtcgtg tcgctgttcg
      361 actgcgaaga gagccacgcc gcctattacc tgacccggca ggggatcgag cagttcgatg
      421 tgatgaagtt catatcccac gggctcggcg cgctcagcga agaggtggca cacgagcagg
      481 aacaggccga tcgcgaggcg gctcgcccgc cacgcaaaaa ggagcgggac cgcgaaaagg
      541 agagggatga gggcgatccc aagggcggca tcgaatcgta cgcggtcgat ctcaccgcac
      601 tcgccgagaa gaaagcgctg gagccgctca tcggccgcgc cgacatactg acacgcacca
      661 tgcaggtgct ttgccgcaaa acgaagaaca atcccatcgt cgtcggtgag ccgggggtcg
      721 gcaagaccgc catcagcgag gggctcgctc agcgcatcgt cgcggggacc gtgcccgcgc
      781 cgctcaagga ccaccggatg ttccgtatag acatgggctc gatgcttgcc gggaccaagt
      841 accgcggcga cttcgaggcg cgcatgaaga aggtcctgag ttcgctcgcc cagcgagaga
      901 aggtcatcat cgtgatagac gagatacata ccatcgtcgg cgcgggcgcc gtgtcgggcg
      961 gctcgatgga cgcgtcgaac ctgctcaaac ccatcctcac caccgggcgg ctcaagtgca
     1021 tcggctcgac cacccacgag gagtaccgca aatatttcga aaaggaccgc gcgctcagcc
     1081 gccggttcca gaagatagat ctgcccgaaa cgacgataga ggaaacggtc gacatactca
     1141 aggggatacg ctcccgttac gaggagtacc atgcggtcaa gtacaccgat acggcgctcc
     1201 gcgcggcggc cgaactgtcg gcccgttatg tcaccgaacg gttcctcccc gataaggcga
     1261 tagacctcgt cgacgaggcg ggcgccttca cccgcatatc gggggaggag cgcaagacca
     1321 tcgaggtgtc ggacatcgaa cgggtcgtgg cgctcgtcgc gcggataccg gaaaagacgg
     1381 tgagttcggg tgaaggcgag aaactgcagg ggcttgaggc ggcgctgaga cgacagatat
     1441 tcgggcagga tggcgcggtg cacgcggtcg tcgaggcgat aaaacggtcg cgcgccggct
     1501 tcgggcggcc cgaacggccg gtggcgtcat tcctcttcat cgggccgacc ggcgtcggca
     1561 agaccgaatt ggcgcggcaa ctcgcccgcg aactcggcgt gacgctccac cggttcgaca
     1621 tgtcggagta tcaggagaaa cacaccgtga gccggcttgt cggcgcgccg cccggctatg
     1681 tgggctacga ggagggagga ctcctcaccg aggcgatacg caaaacgcca cacgcggtgc
     1741 tcctgctcga cgagatagag aaggcgcacg ccgatatcta caacacgctc ctgcagatca
     1801 tggactacgc gacgctcacc gacaacgcgg ggcgcaaggc cgatttccgt aatgtcatca
     1861 tcatcatgac ctcgaatgcc ggtgcgcgcg agatcggcaa ggcggtcccc gggttcggcg
     1921 agcgggcgct ggatggcggc gaactcgacc gtgccgtcga gcggatattc tctcccgagt
     1981 tccgcaaccg gctcgaccgc gtcgtgcggt tcgagcggct cgacctcgac atcatcaccg
     2041 atatcgtgaa gaagcaggtg gctgaattcg ccgaacagtt gcgagagaag aaggtcgaat
     2101 tcaccgtcac cgcggcggct taccgccatc ttgccgaacg cgggttctcg aaggagttcg
     2161 gtgctcgcga ggtggggcgg ctcatacagg aggaggtcaa ggacttcttc gtggacgagg
     2221 tgctgttcgg gcgtcttcgc aacggcggaa aggcccgcgc gatattccgt ggcggccggt
     2281 tctccatcga ggtggtctcc tgacccatgg tctatcagat aacccgtgac cgcatcgcct
     2341 tccccgaccc gcggctcgcc gaggaggacg gtctgctcgc catcggcggc gatctttcgg
     2401 tcgatcggct tcttgccgcc taccggcagg gtatctaccc gtggtacacc gacgaatatc
     2461 cgatactctg gtggagcccc gatccgcggt tcgtcctctt cccggaggaa ctcagggtgt
     2521 cacggtcgat cgctaaactc ctgcgtcata acgacttccg ggtgacggtc aaccgggcct
     2581 tccggcaggt cgttcgatcc tgcgccaagg tgccgcgccg cggacagcag gggacctgga
     2641 taacggccga catgatagcg gcctaccagc ggctgttcga aaaaggccat gccgtcagcg
     2701 tcgaatcgtg gctgggcgac gaactggcgg gagggcttta cggcgtcatc atcgggcggt
     2761 gtttcttcgg cgagtcgatg ttcgcgtggg agccgaacgc atcgaagatc gccttcgtca
     2821 agttcgtcga atttttgagg gagaacggca tccgcgtcat agactgtcag atgcgcaccg
     2881 cgcacctcga gcggttcggc ggccggttca taccgctcgc tcactttctc gatatacttt
     2941 ccggtggttg tgacgaaccg atgccctcct ggcccgtttc cctgtggtcc gaaaaaactt
     3001 gaagcgattt ttccgcacga tataatacgc cgtctttgca cgataaccga aagaggaggg
     3061 tatcatgaaa tggttcacga tggtcgctgt cccggcgatg ttcacggtcc tgttcctttc
     3121 ctgtgaaggg accaagacca ccacacagga caaggatata tccctcggcg atgaggactc
     3181 gcttcttgcc gatgagtcgg tgaccgacga ggaggagccc gagggcgagg agacggtcaa
     3241 ggatgacggc ccggtcgcca aggatgacgg cccggtggcg aaggacgacg gcccggtggt
     3301 gaccgatgat gccccggtgg tgaccgatga cggcacggtc gagccggacg aggatatcgt
     3361 ggtgaccgac gacatcgtca ccgacgatgt ggtgaccgat gatgtcgtta ccgacgatgt
     3421 ggtcactgac gatgtggtca ctgacgatgc ggtgaccgat gatatccagc ccgacgacga
     3481 tcagatagag accttcacga tcggctggtg taacctgcag tacccggcgg atgccacggc
     3541 gacacagggc gataatgtga ccttctacgg ccgcgtctac atcgcggggc tgaccgacct
     3601 gaccgccgag gttgatacca acgcgcaggt catcggccag ttgagcggca acccctcatc
     3661 gggcgtcgat gcggcgacct ggccatccga cggctggctc gacctgttgc ccaatccgga
     3721 caatgagacg gcggtagcca tcggcaacaa cgatgaatac atgctcgagg tcccggtcat
     3781 ggccgatccc ggtgtcatgg acatcacggt acgattctcg ggtgacggtg gtgccacctg
     3841 gacctactgc aacgcgaacc gtgaggggag cgaggggtac aacggcaccg atgtcgaaaa
     3901 tccgtatgac cccgcgaaac acggccacct gacggtcgaa gcggcctacg acgatccgtg
     3961 cgacggggac aacccctgcg aagaaccgaa ccgttcggtc tgcacagacg cgaacacgga
     4021 tggcgtcgcc gaatgcgcat gcgacgagaa ctatacgctc gacggtggcg acacctgcat
     4081 caacgagaag ttcgtcgact gcgccgcgaa cccgtcaaaa ccgctgaaca gccacgatgt
     4141 cataacgcag gtgaaggtga actacaccga cctcgacggg tggggcgacc ccgagatctg
     4201 caccacatgg gactgcaacg acggctatca ctggaacgag gacgaggagg cctgtcttga
     4261 gacgcttacg atcggctggt gtaacacgca gtggccgacc gacgcgtcgt ggtatcgcgg
     4321 cgaagaggtc gcggtctacg ggcgtctcta tatcgcgggg ctcaccgatc agagtcttga
     4381 tgtggacgag gcgccgcagg tcatcgtgca ggtcggttcg tccccgaccg gaacgagtgc
     4441 gaccgactgg atgtactggg gagcgatggt cccgaacccc gacagcaaac tggctcccgg
     4501 tatcggcgag gatgaggacg agtaccaatt catcggcgcg gtgaccgaaa cgctcggcga
     4561 ctgggaccac ctcgttcgcg tatcgggcga cagcggcgcg acctggacct actgcaacgc
     4621 gaaccgcccg ggcgaaggat ataacgggac cgactcggac aatccgtacg accttaccaa
     4681 gaacggccat ttcacggtga ccagcccgtg tggcgaaggg ttctgtgatg agccgaacaa
     4741 gtcggtctgc gccgacagcg acagtgagcc gttctacacc tgttcctgcg acacgcacta
     4801 caacgacgac gacggtaatg gtacctgcga ggccgacagg cagttggtcg attgtatgaa
     4861 cgccctgccg gagaacgccg tttgggatga ggatctgccg atctatgagg gactcggtaa
     4921 actgtggcag acatggaacg atggctggaa accggaggcg gacacctgcc cgtgggtatg
     4981 cgccgcgacc tatcacgaag aagatggcga ctgtgtttcc gatgcgcctc gaaccgtgaa
     5041 ttgtatcgat aataagcccg agaccagtca ctgggtcgct gacggtgcct acaacggtga
     5101 cggtacggtg ggtcagacct ggaccggcac cgaatggata ccgcctgccg acgaatgtcc
     5161 gtgggagtgc gacaccgact atcactatga gaactcgagc gaacagtgcg tgaccaacac
     5221 catcgggcgg ctctgcacca acacgcttcc gacgggtgcc gattgggtcg ttctcgcgcc
     5281 ctatgatgga gaagggaatc ttatccagac atgggtcggt cccggcgacg acgactggac
     5341 gcccgacacc gacacctgtc cgtgggaatg cactgaaccc ggttatcacg agaacaccga
     5401 ggtcaatcag tgtctcctga cctacgcgat cggctggtgt aaaattcagt ggccgcctac
     5461 cttcgagggt attcgtggaa gcagtggcga gacagtatac agtcgtttgt gggtggatgg
     5521 agtaaccgac atgacggccg gtcccgatcc cttgccccag ataaaggcgc aactcggcgc
     5581 cggtccgcgt gacaccgatc cgagcacttg gggtgatttg tggatgcctg cggtgccgaa
     5641 tggcgagtac tccccttcga gcccggacta cagtccgaac aacgatgagt atatggcaat
     5701 gccggttctc gatatgccgg gcggtctgta cgattatgca tatcgtttct cgggagacag
     5761 cggtcaaaca tggacctact gcgacctgaa cgggagtagc gatggctaca cgaccgatca
     5821 ggcgggcaga ctggcggtgt atgagtgtga tgccagcgaa gattgtacct acgaggcggg
     5881 caaggatatc tgcgtcaccg acgacaccga tccgatgtac cggatgtgcg tggagtgtct
     5941 ggtgacggat gactgtgcgg aaggtaatgt gtgcgatacg gcgagttata cctgcgtcga
     6001 gtgtttggat aacaccgatt gtaccgacga ggtcggtctg ccggtgtgtg acacggccaa
     6061 ccacaattgt gtcgagtgcg tcgaagatgc tgattgcgtc gacccgttag catactgcac
     6121 agttgataat gcctgtgagg tgccgcccat cggcaagttc gccgactggc cgttgacgac
     6181 cgatggatcg ccgaccaatg tagctacggg tgtgttggcc ggaagttaca gtggaggtac
     6241 aggcatcgga ttaataactt atggagtgaa tggggcatat gccagcaatt ggaccacagc
     6301 tctttcccca gacgccgatg attattttca gattaccgtc actcctgata ccggagtgtc
     6361 ttcaatagtc atcacctcaa tcaactattc ggagcgacgt tcttcaaccg ggatacggac
     6421 ctatgagatt cagtattcaa ttgcttctga tttttctgat ccggtggttc tggatacggt
     6481 tactgtccct gatgacgatc tgacgcgttc tcatgcgctg acagatttgt ctattgccgt
     6541 agatcaggga gagaccctat atcttcgctt cttcggctat gcggccgaag catctggtgg
     6601 cacttggcgt atcggatcaa atgctctgaa gcttgagggg cgcatctatc ctgaataagt
     6661 cctacttccc gaaaagttcg aagtaactct tctccgtcag aaaaaaattg caaagaggaa
     6721 gggagtcttt ataatcattc ctttgatgag ttgaaagcaa attcccatgg aggaagcaat
     6781 gcggaatctc gtgctgtttt cggcctgcgt ggccctgttt ctggtcgtcg gctgtgcgga
     6841 gaagaaaaag gagccgtcgg gcgagatcgt gaaaaaaatg gtgacggcgg ccggaggcgg
     6901 caccgtctca tgggccgatg gatcggcctc ggtcgatata ccggccggtg cgctggcggt
     6961 cgataccgag ataacgatag agaaaaagga caaaaaggat tttccggaga cggagaaact
     7021 gtacagcgcg gtctggaatt tcgggcccgc ggggctgacc ttttcgaagc cgatcaggat
     7081 gaccatcaag accgacaagg cgaagggagc ggggcctgtg gcggtcgtgg cggtcctgcg
     7141 caacgatgcg tggcactcga agggatatga cggcattcac gccgcggggg atccgatcat
     7201 ggcgggcgac ccgatcatgg cgggcgaccc gatcatggcg ggggatccga tcatggcggg
     7261 cgatcccatc atggccgggg tggggcactt ttcctccttt gtcgtgtatg tacaggccgg
     7321 ggacgatatc ttgaccgaca gcgatatcgt cacggaggaa gggccggtcg agaacgagga
     7381 tctacctgaa aacgacgcgg acgaactgct gtcggacgac gacgggcccg ggctttgtgt
     7441 cgagaatgag acaaagagcg aagggggcga ttatcttgta tgtcacagcg gcgagtggat
     7501 cataaaacgg gaaacgcgcc agtggggaac ggcagagcag gatatcgcct acggcatcgc
     7561 ccggtcgagc gatgacgcgg tctatgtggc cggtctcact aacggagatc tggaggggaa
     7621 tctctcggcc ggtctcagcg atcctttcct gacaaagtgg tcgagcggag gcgtcatgga
     7681 gtggacggtg caatggggaa ccgccgcgac cgacgaggcg cgcgccgttg ccgtcgcgga
     7741 agacggatcg atctatgtgt cgggagggac ccgcggtgcg ctcgatggga acaccttggc
     7801 gggcgactac gatatttttc tgacaaaatg gacggccgac ggcgtgaaag agtggacccg
     7861 tcagtggggc accggcgcat ttgaccaagg ccgggcgatc ggggttgacg gcgcggggaa
     7921 tatctatgtg accggagaaa cgctggggaa actcgacgga aatgtcagtg ccgggggcta
     7981 cgatgctttt ttgacgaaat ggtctcccga cggtaccaaa cagtggacgc ggcagtgggg
     8041 ttcggcgacc tatgattatc cgatgggtct gacggtcggc ggtgatggaa gcgtttatgt
     8101 cgcggggcgg gccgataacg cgatggacgg cgagccgcat gccggcggtc gcgatccctt
     8161 cctcacgaaa tggtcttccg acggtgccaa acagtggacg cggcagtggg gaagcgccaa
     8221 tgacgatcag gcgtcggcgg taagcgtgtc gggcgcggtg ctctacatcg ccggatatac
     8281 cgctggaacg ctgtccgatc aggtctcgaa gggggatcgg gactttttcg tctccgcctt
     8341 caccgatacc ggggagttcc tctggaccct gcaggagggg gaggagacag aagataccgc
     8401 caccgctatc acggttgata ccgcgggaga gatatggatc acggggtata cgcggatcgg
     8461 cgtcaacgac aacttcctcg cccgtgcgaa cgatcagggg gagatgcttt ccttcctcca
     8521 atggggcggc cctcaggtga ccctcgtcga tcaggcgaac ggactgacga tatcctctct
     8581 gggagagata ttcgtgaccg gtcagaccga cggcaagatg gacgattcac cggccactac
     8641 cggaggggag gagatatttc ttacccgact ggtggtcgag tgatcttccc gaaaagttcg
     8701 aagtaattct tggccaccgt ctcggggtcg aactctttca tgatatgttc atgtcccgcc
     8761 tttgacaggc ggtcgcgggc atcgacatcg ttgagaagcg ccgcgatctt cgccgcgaag
     8821 aagtcggtgt cgaagggagg gaccaccacg ccggtcacgc cgtcaatggt gttctcggcg
     8881 agcggcccca cgccgaagag taccggcacc gcgccgcagg atgccgcttc gagcggtgtc
     8941 aaaccgaaac tctcggcctg cgaggggaag agcaggatat cggcggtgct gtaaagagtc
     9001 ggcagatcag cggcggggag gaagccgacg gcgcggacct tgatgttgcg atagtcggtc
     9061 gggaagggga gtt
//
LOCUS       DAOQUE010000049         8962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8671_length_8962_cov_4.232177, whole genome shotgun sequence.
ACCESSION   DAOQUE010000049 DAOQUE010000000
VERSION     DAOQUE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8671_length_8962_cov_4.232177"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /gene="amrB"
                     /locus_tag="PKH10_02560"
     CDS             <1..1311
                     /gene="amrB"
                     /locus_tag="PKH10_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNT27039.1"
                     /translation="QPFVIVAPHAGFVYSGRVAAHAYAGLKPKQFDVVVILGTSHSFQ
                     NGVVALSPAAAFRTPLGTVPVDRAMTDRLARADKRFVQSAEIHGPEHSIEAQLPFLQE
                     RLGNFSMVPVLTATADRATLDRFAKELIAVIEASKKKVLIVVSTDLSHYHDYHTAAKM
                     DGETLHLISEGKWEELRGKIASGACEACGYHAFHLAARILEHYGVTSGTLVKAENSGD
                     VVQESRPGGVVGYGAVIFEKNKGGTVNEGSKPGAQTTTEERKYLLDLARKSIEHALRT
                     GKEFVPEKPAKGVLTADRAVFVTLKKHGDLRGCIGQLEARGPLYLAVAEMANSAAFHD
                     PRFTPVKQDEMRDIEIEISILTPPQRIDTYKKVVLKRDGVIARNGYRGGVFLPQVAEE
                     TGWDIDTFLGELCAQKAGLPRDCYKDNATQLSVFQVELFSEKNY"
     gene            complement(1318..1392)
                     /locus_tag="PKH10_02565"
     tRNA            complement(1318..1392)
                     /locus_tag="PKH10_02565"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1357..1359),aa:Glu,seq:ttc)
     gene            complement(1405..1480)
                     /locus_tag="PKH10_02570"
     tRNA            complement(1405..1480)
                     /locus_tag="PKH10_02570"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1445..1447),aa:Lys,seq:ttt)
     gene            1634..2215
                     /locus_tag="PKH10_02575"
     CDS             1634..2215
                     /locus_tag="PKH10_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT27040.1"
                     /translation="MKEHLKVEIVKMAKTLLNEGKDIRHITMNLIAKRMDITAPTLYH
                     YFRNKEEIIHLSLELIADEIVATFEQPFPASMDNEMRFKMTIVNLMSYLYRNIESFAL
                     AFSDVGIGYWGSGEIKMSDKIRKARSLLEEEVRRYYQSAKIPHDPLKGTYILLSLMMG
                     SLRYFRSQKIKNADIGREAEEVFSFFVKGLQKG"
     gene            complement(2216..3973)
                     /gene="uvrC"
                     /locus_tag="PKH10_02580"
     CDS             complement(2216..3973)
                     /gene="uvrC"
                     /locus_tag="PKH10_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNT27041.1"
                     /translation="MPIDQPSFGPPDPPHIPPEPGVYLFLDETGAVLYVGKARNLKSR
                     VRQYFAGHDERPLVPFLMRRVTDIRVIVVRDEREALVLERRLIKRHRPPYNIDLKDDK
                     SYPYLAFTDEKFPFLVVTRKPHLRYRYLRGPFTDAALLRRFRRALLACHPLRRCIKMP
                     AKACVYHQIGLCPAPCEGRIAADEYDQRVRRVIALIEGRDWREFGAAVTVEIERAAAA
                     LRFEKAAELRDILRLLPELGTRFGVESARRRAEDTFFFSNDGLTLFLTVGSYADGALG
                     DIYHFHHPLTPGEPLFYPAIASFYAHRPPPAALSCHPPEAVEADALERLLDAKVDILP
                     MRPEVRELLLRNHSHFKERLSRIGSAGPKARDALAAVVGAPVESILCLDISTLQGSHT
                     VAGAVWWEGGRFVTKHYRRFTIRTIEGQNDPAAIAEAASRFRAHWENGDWPKPDLVLV
                     DGGTTQVQAALREIGDGTALLGIVKDRRGDRGGEKLVAPTGEETLLDDGPFALLLKRI
                     RDEAHRFAITANRTKRKEAVADLLGGIPGIGPAREKVLIGQFRTVERIRSATREELRA
                     VPGLTQEAADAVWRRFHEG"
     gene            4177..4428
                     /locus_tag="PKH10_02585"
     CDS             4177..4428
                     /locus_tag="PKH10_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNT27042.1"
                     /translation="MTVQIYVGNLPFSATEGDLRSAFEKFGAVSSAKIVTDRDTGRAK
                     GFGFVEMADQAAADKAISSLDGADMGGRNIKVNIARPKK"
     gene            4563..5999
                     /locus_tag="PKH10_02590"
     CDS             4563..5999
                     /locus_tag="PKH10_02590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT27043.1"
                     /translation="MPDTTLPSILLVLDSAPEEPGAPNYQNAWIAELVAELGAACRLS
                     LFYPVLSVGGPFYRLRREERGGVERFVASIPYKYPSFAETYDNPEMDRLFASVIGERA
                     FDAVHILSLRNHSFGYPETAWRAGLPVVLSAFDGWLRCPCRYRTGCDGTERDAHRCRV
                     RLSNFVATPISFLVKRVEHALRAGGHHWWFEETGRYSAFYNRGPCERVGEEALAERDR
                     RSREMLPYVDAFHFPSEEFHKSFFSDLVPSQRAFFMGQGIPAALVVDSRPFEIPGGVR
                     FGFIGDIVPEEGLTELVEAFNALRERGLPNSLHLYGELYRNEGYVNRLRKECRNGAVV
                     FQGAVDPGRIGAVLDSFDVLVLPSRWQRPDSWLAAQAMARRKAVIAPAGTAAAELIRK
                     RRRGVVLKNLSARDLEQIFVDLEIDRKKLYYLMRIVEREQFLSTAGNARDLLARYRSL
                     MKRPARTEEKVLRRRLNRKRTERSRGAA"
     gene            5996..6682
                     /locus_tag="PKH10_02595"
     CDS             5996..6682
                     /locus_tag="PKH10_02595"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNT27044.1"
                     /translation="MTTTGKRVDIELTTLGKAFVIFILLLVLSFVIGLYLGREAGKRV
                     STPLDDASRDEQVSACNYKVQDLTTRLTALTAAAKEAGLMDGQGSVARGVRCAVPEKG
                     TVPAVAAIPEKEKEKKDEAKEGVKPIGPGNSSPGETAAAKVPPVVPEKTAPAACKFSL
                     QIYASKSKEEALAVQKRSKIQPLRLVEAEIDGAVWYRLRYGCFAEKGTAEAEIEGLMK
                     QGARPYLAKE"
     gene            6709..8712
                     /locus_tag="PKH10_02600"
     CDS             6709..8712
                     /locus_tag="PKH10_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27045.1"
                     /translation="MVRYVVALSMMLLFGALWGQEVAPVAPEGEPVTPLEEAIAPVSA
                     ETPPAEATPSAETPPAETPPEGTPPAEPSSPLMEIPPAPEPTPLPTPVPVAETPGSAE
                     SSAVASPPAEAAPVAEAAPAAEAVPSAEPSPEEKAEAEKKAEEEKKEKEKKKFVKGEI
                     SNIGARDIVYPYNSLGIGTGVTFIAQDIFLTLDPRFVYNKNDFNMLMAFHLPINIRMF
                     SFDLNRAGDDNFTLRSEDWDEWQDYFRIIRYVQYGRSEDNFYASIGSNNAQSIGHGTI
                     MKRYVPNFDPNFTKISAKLNYYGDYFGFESVLGDVTDGNMFGGLVFFKPFSGLAEDEN
                     DYMPRSFSIGFSFAGDRKAPTKIEYVDYVFGTPGGDTYTADGNDPRWPAGLVPRIEVD
                     GKGRPVVLEDTFLYLMGVDAEFKFFKNEYVDLKTYIDYSWMRTDPGRGGFTAGLLGRY
                     SPDEDRKHALRSRIEARAYNGRYAPSFFDTFYDIERFELLTNIGGEDGFRPDGRSKLL
                     RLDDQPDKFFFSLYAELTYSFIDWVTISMGIEQLRESLSIYAHLELPDLWIFKGMLSY
                     YQRGITKPETMVDTFDYSAMFRGVIRLQVLPILFINAYVGKQWAFWPPNKPRDDGLQG
                     HYLSAWDLGGDLEIGWEWGDDDEEEAEGTKDDEEKKEDEESIL"
     gene            8734..>8962
                     /locus_tag="PKH10_02605"
     CDS             8734..>8962
                     /locus_tag="PKH10_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27046.1"
                     /translation="MLRTTLCLIAMLCWLTAGAAEVYVNGVKADGLTAQEFKNCTVRF
                     DKKRTVHITAPDVKLLAEGTPTAPAKVAAQYF"
BASE COUNT         1764 a         2748 c         2819 g         1631 t
ORIGIN      
        1 cagccctttg tcatcgtcgc gccacacgcc gggttcgtct attcgggccg ggtggcagcc
       61 cacgcctacg ccggtctgaa gccgaaacag ttcgatgtcg tcgtcatact cggcacaagc
      121 cactcgttcc agaacggcgt cgtggcgctc tctcccgccg ccgcgttccg gacaccgctc
      181 gggaccgtgc cggtcgaccg cgcgatgacc gaccggctcg cgagggcgga taagcgtttc
      241 gtccaaagcg ccgagatcca tggaccggaa cacagcattg aggcgcaact cccgttcctg
      301 caggagcggc tcgggaactt ctcgatggtg ccggtgctca ccgcgaccgc cgaccgcgcg
      361 acgctcgacc ggttcgcgaa ggaactgatc gccgtgatcg aggcatcaaa aaagaaagtg
      421 ctcatcgtcg tgagcaccga tctgtcgcac taccacgatt atcacaccgc cgccaagatg
      481 gacggcgaaa cgcttcatct cataagcgag gggaagtggg aagagttgcg cggcaagata
      541 gcgtcgggcg cctgtgaggc gtgcggctat cacgcctttc acctcgcggc acggatactc
      601 gaacactacg gcgtgaccag cggtacgctc gtcaaagcgg agaactcggg cgatgtcgtg
      661 caggaatcgc gtcccggcgg ggtggttggc tacggcgccg tcatcttcga gaaaaacaag
      721 ggcggcacgg tgaatgaggg atcaaagccg ggtgcccaga cgaccactga ggaacggaaa
      781 tatcttcttg acctggcgcg caaaagcata gaacatgcct tgagaaccgg gaaggagttc
      841 gttcccgaaa aaccggcgaa gggggtactg accgccgacc gcgccgtctt cgtgacgctc
      901 aaaaagcatg gcgacctgcg cggctgcatc gggcaactcg aggcgcgcgg cccgctctat
      961 ctggcggtcg ccgagatggc gaactcggcc gccttccacg atccgcgctt tactccggtc
     1021 aagcaggatg agatgcggga tatcgagata gagatatcga tactcacccc gccgcagagg
     1081 atcgacacct acaaaaaggt ggtactcaag cgcgacggcg tcatcgcccg gaacggctat
     1141 cgcggcggtg tctttctgcc gcaggtcgcc gaggagaccg ggtgggacat cgacaccttc
     1201 ctcggcgaac tctgcgcaca gaaggcggga ctcccgcgcg actgctacaa ggacaacgcc
     1261 acccagctgt cggtctttca ggtcgaactg ttcagcgaga agaactactg aaaaaagtgg
     1321 cgaccccaac cggatttgaa ccggtgctac cgacgtgaaa ggccggcgtc ctaaccgcta
     1381 gacgatgggg tcgtcccaaa aaagtggtga gccggcgggg attcgaaccc aggaccacct
     1441 aattaaaagt caggtgctct acctactgag ctaccggccc acagcgggct gaaaatcgag
     1501 tcgctactat aatgggcggt cttccgttgt caaccttttt gaaaagagaa agggataaat
     1561 ttgttttttg tctttttatt gtataacatt agtcgttgag cgtccttaat tagatacatt
     1621 ttcggaggca gagatgaaag agcacctcaa agtggagatc gtgaagatgg ctaagaccct
     1681 cctcaacgag ggaaaggaca tccggcatat caccatgaac ctcatcgcga agcggatgga
     1741 catcaccgcg ccgaccctat atcactattt ccgcaacaag gaggagatca tccacctgag
     1801 tctcgaactc atcgccgacg agatagtcgc caccttcgaa cagccctttc cggcgagcat
     1861 ggacaacgag atgcgcttca agatgaccat cgtgaacctc atgagttatc tttacaggaa
     1921 catcgagtcg ttcgcgctgg ccttctccga tgttggcatc ggctactggg gttcgggtga
     1981 gataaaaatg agcgataaga tacgcaaggc ccgctcgctc ctcgaggagg aggtgcgccg
     2041 ttactatcag agcgccaaga taccgcacga tccgctcaag gggacctaca tcctgctgtc
     2101 gctcatgatg ggctcgctcc gctacttcag gagccagaag ataaagaacg ccgacatcgg
     2161 cagggaggcc gaagaggtct tctcgttctt cgtgaagggt ctccaaaagg gttgatcacc
     2221 cttcgtggaa ccggcgccac accgcgtcgg ctgcctcctg cgtcagcccc ggcaccgccc
     2281 gcaactcctc ccgcgtcgcg gatcgtatcc gctccactgt ccggaattgt ccgatgagca
     2341 ctttttcgcg cgccggtccg atacccggta tgccgccgag cagatcggcg accgcctctt
     2401 tccgcttcgt ccggttcgcc gttattgcga agcggtgcgc ctcgtcgcgt atccgcttga
     2461 gcaggagcgc gaatgggccg tcgtcgagga gcgtctcttc acccgtcggt gcgaccagtt
     2521 tctcgccgcc gcgatcgccg cgccggtcct tgacgatgcc gagcagggcg gtcccgtccc
     2581 cgatctcgcg gagcgccgcc tgcacctgcg tcgttccgcc gtccacgagc accagatcgg
     2641 gcttcggcca gtcgccgttc tcccagtggg cgcggaaccg gctcgccgcc tcggcgatgg
     2701 cggccggatc gttctggcct tcgatcgtcc gtatcgtgaa acgacggtag tgcttcgtca
     2761 cgaaccggcc tccttcccac cagacggcgc ccgccaccgt gtgggatcct tgcagggtgg
     2821 atatatcaag acagagtatg ctctcgaccg gcgcgccgac gaccgcggcg agcgcgtcgc
     2881 gggccttcgg acccgccgaa ccgatgcggg agagtcgctc cttgaagtgg ctatggttgc
     2941 gtaggagcag ttcgcgtacc tccggtcgca tcgggaggat atccaccttc gcgtcgagca
     3001 gtcgttccag cgcgtcggcc tcgaccgcct cggggggatg gcacgacagc gcggcgggcg
     3061 gcgggcgatg ggcgtagaac gatgcgatgg cgggatagaa caacggctcg cccggcgtga
     3121 gcgggtgatg gaaatgatag atgtcaccca gcgcgccgtc ggcgtaggag ccgacggtca
     3181 ggaagagcgt gagcccgtcg ttcgagaaga agaacgtgtc ctcggcccgg cgtcgcgccg
     3241 actcgacccc gaaccgcgtg ccgagttccg ggagcaatcg aaggatgtcg cgcagttcgg
     3301 ccgccttctc gaaccgtaga gccgccgccg cccgctcgat ctcgacggtg accgccgcgc
     3361 cgaactcgcg ccagtcgcgt ccctcgatga gcgcgatgac ccgacgaacg cgttgatcgt
     3421 actcgtcggc cgcgatgcgc ccttcgcacg gggcggggca caggccgatc tggtggtaga
     3481 cgcaggcctt cgcgggcatc tttatacagc ggcggagcgg atgacaggcg agcagggcgc
     3541 gccggaaccg gcgcaggagc gccgcgtcgg tgaagggacc gcgcaggtaa cggtaccgga
     3601 ggtgcggctt gcgcgtgacc accaggaacg ggaatttctc gtcggtgaag gcgaggtagg
     3661 gataactctt gtcgtccttg aggtcgatgt tgtacggggg gcggtgccgc ttgatgagcc
     3721 gccgctcgag cacgagcgcc tcccgctcgt cgcgcacgac gatgacgcgg atatcggtga
     3781 cccgccgcat caggaacggg acgagcggtc gctcgtcgtg ccccgcgaaa tactgccgga
     3841 cgcggctctt caggttgcgc gccttcccga catacagcac cgcgcccgtc tcgtcgagga
     3901 atagatagac gccgggctct ggggggatgt ggggcgggtc aggaggaccg aacgacggtt
     3961 gatcaatggg catggacgag gtccctctcc ggtgaacgct tgagcatacc ggagcggcgc
     4021 cggtaaagca agcgagggcg cgcgcttttc tcttgcaaat aatattggaa acttatactt
     4081 gctttttttt gataacggag tatgttccct tccgttttgg gttcttctcg gttgagagga
     4141 tccggttttt tctttttttt tattgaggta cagaccatga ccgttcagat ctatgttggc
     4201 aaccttccgt tcagcgcgac cgaaggcgat ctccgtagcg ctttcgagaa attcggcgcc
     4261 gtttcctcgg cgaagatcgt caccgaccgt gacaccggca gagccaaggg tttcggcttc
     4321 gtcgaaatgg ccgatcaggc cgccgccgac aaggcgatca gttctcttga cggcgccgac
     4381 atgggcggcc gcaacatcaa ggtcaacatc gcccgtccca agaaataatc attctcatta
     4441 tctcttgatc gtgggggcgg cgacgggccg ccccttcttg ttcggctctt ctttgacttt
     4501 cccctcttcc tctgatatca tcgcgccgtc gtcttttttg tcgttcatgc gaggagcgcc
     4561 gggtgcctga caccacactg ccttccatac tgctcgtact cgattcggcg ccggaagaac
     4621 cgggtgcgcc caactaccag aacgcgtgga tcgccgaact tgtcgcggaa ctcggcgcgg
     4681 cctgccgtct gtcgctgttt tatccggtcc tgtcggtcgg cgggcccttc taccgcctgc
     4741 gccgtgagga gcgcggcggc gtagagcggt tcgttgcctc gataccctac aagtatccat
     4801 ccttcgccga gacctacgac aaccccgaga tggaccggct gttcgcctcg gtcatcggcg
     4861 agcgtgcctt cgacgcggtc cacatccttt ccctgcgcaa ccactcgttc gggtatcccg
     4921 aaacggcgtg gcgcgccggt ctgccggtgg tgctaagcgc ctttgatggc tggctgcggt
     4981 gcccgtgccg ctatcggacg gggtgcgacg gaacggaacg ggacgctcac cggtgccgcg
     5041 tgagactgtc gaacttcgtc gccacgccga tatccttcct cgtcaagcga gtggagcacg
     5101 cgcttcgcgc cgggggccac cactggtggt tcgaggagac ggggcggtat tcggccttct
     5161 acaaccgggg accgtgcgaa cgggtgggag aggaggcgct tgcggagcgc gatcgacggt
     5221 cccgtgagat gttgccgtat gtcgatgcgt tccacttccc ctccgaggag ttccacaaat
     5281 ccttcttttc cgaccttgtc ccgtcccagc gtgcgttctt catgggtcag gggataccgg
     5341 ccgcgctcgt ggtcgacagc cgcccgttcg agatcccggg cggggtgcgt ttcggcttca
     5401 tcggcgatat cgtgccggag gaggggctca ccgaactggt ggaggcgttc aacgccctgc
     5461 gggagcgcgg tctccccaac agtctccacc tgtacgggga actataccgc aacgagggct
     5521 atgtgaacag actgcgcaag gaatgccgta acggcgcggt cgtttttcag ggggcggtgg
     5581 acccgggacg catcggggcg gtactcgata gtttcgatgt gctcgtgctc ccgtcccgct
     5641 ggcagcggcc cgattcgtgg cttgcggcac aggcgatggc gcggcgcaaa gcggtgatcg
     5701 cgcccgccgg gaccgcggcg gccgaactga taagaaagcg gcggcgcggt gtggtgctga
     5761 agaacctttc ggcacgcgat ctggagcaga tattcgtgga ccttgaaata gacagaaaaa
     5821 agttgtacta tctcatgcgc atcgtcgagc gggaacagtt cctctcgacg gcggggaacg
     5881 cccgcgacct gctggcgcgg taccgttccc tgatgaaacg acctgcgcgt accgaggaga
     5941 aggtattgag acgacggctc aaccgtaaac ggaccgaacg gtcacggggg gcggcatgac
     6001 gacgacgggc aaacgggtcg acatcgaact gacgacgcta ggcaaggcgt tcgtgatatt
     6061 catcctcctg ctcgtgctgt cgttcgtcat cgggctctat ctcggtcgcg aggcgggcaa
     6121 acgggtgtcg acgccgctcg acgatgcatc gcgcgacgag caggtgtcgg cctgcaacta
     6181 caaggtgcag gacctcacca cgcggctcac cgcgctcacc gccgccgcga aggaggcggg
     6241 gctcatggac gggcaggggt ctgtggcgcg cggtgtccgg tgcgccgttc ccgaaaaggg
     6301 gacggtaccc gcggtcgcgg cgatccccga aaaagagaaa gagaagaagg atgaggcgaa
     6361 ggagggggtc aagcccatcg gccccgggaa ctcttcgccg ggagaaaccg ccgcggcgaa
     6421 ggtgccgccg gtcgttcccg aaaagacggc gcccgccgcc tgcaagttct cgcttcagat
     6481 atacgcgagc aagagcaaag aggaggcgct cgcggtgcag aagaggtcga agatacagcc
     6541 gctccggctc gtcgaggcag agatagatgg tgcggtgtgg taccggctcc ggtatggctg
     6601 tttcgccgaa aaggggacgg ctgaggccga gatagagggg ctcatgaaac agggggcgcg
     6661 cccctatctg gcgaaggaat gaacatgaac acgggaagga gatacagcat ggtacggtat
     6721 gttgtcgcgc tatcgatgat gttgcttttc ggagcgctgt ggggacagga ggtcgcaccg
     6781 gtcgcgccgg agggcgagcc ggtgacccct ctcgaggagg cgatcgcgcc cgtttccgcc
     6841 gagacgcctc ccgccgaggc aacgccgtcc gctgagacgc cgcctgctga aacaccgcct
     6901 gagggaacgc cgcccgccga accatcctct ccgctcatgg agatcccgcc ggcccctgag
     6961 ccgacgccgc tcccgacgcc ggtaccggtc gccgaaacgc ccggctccgc tgaaagctcc
     7021 gccgtggcaa gcccgcccgc tgaagccgcg cccgtcgccg aggccgcgcc tgccgccgag
     7081 gccgtgcctt ccgccgaacc gtcgccggag gagaaggccg aagcggagaa gaaagcggaa
     7141 gaggagaaaa aggaaaaaga gaagaagaag ttcgtcaagg gcgagatatc gaacatcggc
     7201 gcccgcgaca tcgtgtaccc ctataattcg ctcggtatcg ggaccggcgt caccttcatc
     7261 gcgcaggaca tcttcctcac gctcgacccg cggttcgttt acaacaagaa cgacttcaac
     7321 atgcttatgg ccttccacct gccgataaat atccgcatgt tcagtttcga cctcaaccgc
     7381 gccggtgacg acaatttcac cctgcgttcc gaggactggg acgagtggca ggattacttc
     7441 cgcatcatca ggtatgtaca gtacggtcga tcggaggata acttctatgc ctccatcggt
     7501 tcgaacaatg cgcagtcgat cggccacggc accatcatga aacgctatgt gccgaacttc
     7561 gatccgaact tcaccaagat atcggcgaaa ctaaactact atggcgacta cttcggcttc
     7621 gaatcggtac tcggcgacgt gaccgacggc aacatgttcg gtggtctcgt gttcttcaag
     7681 ccgttctccg ggctggccga ggacgagaac gactacatgc cgcgctcgtt ctccatcggg
     7741 ttctcgttcg cgggcgaccg caaggcgccg accaagatag agtatgtgga ctatgtgttc
     7801 ggcacaccgg gcggcgacac ttacaccgcc gacggcaacg atccgcggtg gcccgccggg
     7861 ctcgtcccgc gcatcgaggt cgacggtaag ggacgccccg tcgtgctcga ggacaccttc
     7921 ctctacctca tgggggtcga tgccgagttc aagttcttca agaacgaata tgtcgatctc
     7981 aagacctata tcgactacag ttggatgcgg accgatccgg ggcgcggcgg cttcacggcc
     8041 ggcctgctcg gccgctacag cccggacgag gaccgcaaac acgccctgcg ttcacgtatc
     8101 gaggcccgcg cctacaacgg tcgctacgct ccttcgttct tcgacacctt ctacgacatc
     8161 gagcggttcg aactgctcac caacatcggc ggcgaggacg gcttccgccc cgacggtcgt
     8221 agcaaactgc tccggctcga cgaccagccc gacaagttct tcttctcgct gtacgccgaa
     8281 ctcacctact cgttcataga ctgggtcacg atatcgatgg gtatcgaaca gttgcgcgag
     8341 tcgctgtcga tctatgccca cctcgaactc cccgatctct ggatattcaa ggggatgctc
     8401 tcgtactacc agcgcggcat caccaagccc gaaacgatgg tcgacacctt cgactacagc
     8461 gctatgttcc gcggcgtcat ccgcctgcag gtgctcccca tcctcttcat caacgcctat
     8521 gtgggcaaac agtgggcatt ctggccgccc aacaagccgc gcgacgacgg tctgcagggg
     8581 cactacctct cggcgtggga cctcggcggc gatctcgaga tcggctggga atggggtgac
     8641 gatgacgaag aggaagcgga agggacgaag gacgacgagg aaaagaaaga ggacgaggaa
     8701 agcatactct agaccgaaaa ggagggctgc gggatgttgc gcacgacact ctgccttatc
     8761 gcgatgctat gctggctcac ggcgggcgcc gccgaggtct atgtcaacgg cgtcaaggcc
     8821 gacggcctga ccgcacagga gttcaagaac tgcacggtga ggttcgacaa gaaaagaacg
     8881 gtccatatca cagcgcccga cgtcaaactg ctcgccgagg gaacgccgac cgccccggcg
     8941 aaggtcgcgg cgcaatattt cg
//
LOCUS       DAOQUE010000050         8926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8698_length_8926_cov_4.116785, whole genome shotgun sequence.
ACCESSION   DAOQUE010000050 DAOQUE010000000
VERSION     DAOQUE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8698_length_8926_cov_4.116785"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PKH10_02610"
     CDS             <1..350
                     /locus_tag="PKH10_02610"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNT27047.1"
                     /translation="VLFQGRTYKMYRGMGSLGAMKQGSSDRYFQDQVREDQKFVPEGI
                     EGRVAYKGAVSETIYQLAGGLRSGLGYVGAKNLTELREKARFIRITPAGFKESHVHDV
                     TITKESPNYRAEH"
     gene            complement(360..2480)
                     /locus_tag="PKH10_02615"
     CDS             complement(360..2480)
                     /locus_tag="PKH10_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27048.1"
                     /translation="MKGSPGDLMRLFCAAFFVLLFFVWGTAGRFWTPLAVYAPSFHLL
                     LAGIVFVLLLFRAPLRRILPPLRDVAVARRYLLFPLAAFAATLLIGLFVFQGLPHIQD
                     GIHYRMMADQLAHGRMDHAMHPHYEFFRFLYLIPDGERIASLFLPGYPLFLAPFALFG
                     VAFLANPLLTAANVAIAGRIADDLYGPRVSTAAMALALLSPFLMIMGGTAMAHPFCAF
                     LTLTTVLFFLRARREGSGKRALIFAAVAGFSLGWLAFCRPQNAFLPWLLVLLLHNAHY
                     TGDLLTLRQDIYFNYSEPNDDCFGLGLGRACPNATWRPLPDDGFTLTAAVVVTYHRLA
                     PLIANTLGHPLLFLLIPCAFLFARGGRLRREMFLAAPFLATIGGHFLYYFDGNVFGPR
                     YYYETTFFIMILVALGLVSLADRIATAPRPHLFAAPLGGFIGAAYLFTAVVILPPLFD
                     SYAFGFWGVGRALADEAARQGIHNAAVFIQPEQYIGSGLVAMRHDDWDRNDIVYVRDL
                     GDRPNSALMHHYAPQGRKFYRALFTDIRTATVPPLLTPLAGPDLPPNYLVAEMEDKHY
                     PLRGVPDYCNEYPNRSDLSKYVSFPPPERIGVGFSKRAFFCRFTAPGQFYEFGQQFFL
                     AGRYSIDVVGVTGPEMGAFRVFIDDREIGRVDFTGERYEKVIRQVEATVAEGFRFMRL
                     EPEDAARDTYFLLDLVEFFRRPDE"
     gene            2619..3470
                     /locus_tag="PKH10_02620"
     CDS             2619..3470
                     /locus_tag="PKH10_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNT27049.1"
                     /translation="MSTFRAYRFTEVFLVAGFIFVGSFFAASDARALFGARPALFFLA
                     SYLVTLAVYAFNSWAGSSDDAANPRLAVVPDDRSPAVAAASLIAALALFLIVERRAAP
                     CAAALFVLSALYSYPRFGAKYLPRAGTALHILGGAVQFQIGWLMFGEIGVRSLALALY
                     FGLILSAGHVNHELIDLDADRAAGIRSGAVRYGAPGSIRLHIVICAAALALLIVAALR
                     GMATPVHLAPFAVASLLQMASAVALSLRAAPGQGDFLRHRRLYRILYALAGIVFLAVK
                     FGPSLGA"
     gene            complement(3456..5669)
                     /locus_tag="PKH10_02625"
     CDS             complement(3456..5669)
                     /locus_tag="PKH10_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27050.1"
                     /translation="MKFSRCDMVRAVAALFFTLLFFVWGTAGKFWRPFQLYVPWFYLL
                     FAGLTALFLLPPIRAAARSLSARIVPRRYILFPLLAFCVTLLINRLVFQGLPHIQDSI
                     NYLFMAEQFAAGRLDLPMHPYYEFFRFLYLIPDGEKIYSLFLPGYSLFLVPFAMIGIP
                     FIVNPLLTAANIFLLGRIADDLYGPRVSAAAMALAILSVFLMVMGGTFMAHPFCAALT
                     LGAVLCFLRARRAGSGKAALLYAAAAGFALGWLVFTRPQNALFLALPLALAALFEIRR
                     RGTVARGLVLTAAFLPWLIALLLYNAHYTGDPLLFKQDPYFNYSEPNEFCHRFGIGRG
                     CPHSNWTVLPEEGLTWGHAAYVTYRRLSPLILNTFGHPLLFLLIPCAFLFYRSGRPKK
                     ELFLAALFLFSAGGYFFFYFDGNVFGPRYYYETTFFLAILAALGLVALSDRIAAAPRS
                     HLFAAPLGGLVAAAYLSFAFIVLPDLLTIYSLGFWKVERSLSAEVERQGIHNAVVFID
                     REELIGSGFAVMRHDDWDRNDVIYVRDLGVRPNSALMHYYAAQGRKFYRARYEKIENN
                     ERPPEITPLTDIELPPNYVVAELEDKLYPLTGAPDYCNRYPNRPDLDRYSALPPPGEL
                     GIGFSKGALFCRFTAPGQFYDLGQHLAVAGRYSVEFVGVSGPEMGRFRAFVDGREIGI
                     VDFTGERYEKVTRRVEAELAAGSHRIRLEPETTGRDVYFLIDLVEFFTDAASRPE"
     gene            5894..8116
                     /locus_tag="PKH10_02630"
     CDS             5894..8116
                     /locus_tag="PKH10_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 repeat-containing protein"
                     /protein_id="HNT27051.1"
                     /translation="MGNESRNILKHQCGSNVIILVAALITAACGSGTKASSDDTNGMS
                     WQRISTGGQHTCAIQGDGKLFCWGHNSCGQLGDGTTEDKNTPTHVGSEDDWKSISVGE
                     EHTCGVNGRGELYCWGNNDHQQIGDGTFRDANSPVRIGTNGDWQNVSAGARHTCAVNM
                     SGELYCWGDGEAGQVGDGANEMNRSAPTRVGTESDWQKVSAGIAHTCAVKVSGELYCW
                     GSNLYGQLGDETTESKNTPVHIGVKSDWQSVSTGEKHTCGLKRSGELYCWGNNQKGQL
                     GDETTESKSTPVHIGVKSEWMSVSAGLSHTCAVTMSGEPYCWGQNWDGQLGDGTREDK
                     NTPTRIDFVGEWKSISAGWKHSCGERRDGWLLCWGENQSGQLGKGAFPDKNIPTHIGA
                     ASDWQGVSAGDGHTCGVKKSGELYCWGNAAHGPFTEITLPQDIPKSFDVKSDWQSISA
                     GMYHTCGVKNSGELYCWGDNRFGQLGDGTEENKGAPVRIGMDSDWQSVSARTWHTCGV
                     KKTGKLYCWGWNEYGRLGDGSEFTRNTPTRIGTAIDWDVVSAGEAYSCALKTNGELYC
                     WGHNFHGQLGDGTTEDRNTPTRIGMDSDWESVSAGERYSCALKSSGELYCWGNDESGL
                     PDIEDTGSTTPVRVGTARDWKSISAGMYHTCGVKGIGELYCWGENYFGQLGDGTREYK
                     DVPIRIGAESVWQSVSAGTYHTCGIQGTAELYCWGRNEYGQLGDGSMWEETPQQVEVN
                     "
     gene            complement(8169..>8926)
                     /locus_tag="PKH10_02635"
     CDS             complement(8169..>8926)
                     /locus_tag="PKH10_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT27052.1"
                     /translation="MGTIIFGVFLIATGNFTMDWRERWRTVAALSFVGLFLNTITFHL
                     ALNSISGTLVMIIENLSPLFVFFLSFTLAGVRPTWREVIALLLSLCGMLFIVYGKDTF
                     DFGGGDVNVGIIWAVLAGATFGWYAYFSAELVRPLRDDPVKVVQFLFKIFVISTVLMI
                     PFLVRDCPRPQKADEWFWLVEMGVFQSGGAYLCWNYALRDIPVNTASVLFLFTILFTA
                     LNELLFLSLPLNIFIIGGGILIIAAGRIITRNG"
BASE COUNT         1960 a         2448 c         3046 g         1472 t
ORIGIN      
        1 cggtgctttt tcagggacgc acctataaaa tgtaccgcgg tatgggttcg ctgggggcca
       61 tgaagcaggg gtcgagcgac cgctattttc aggatcaggt ccgcgaggac cagaagttcg
      121 tgccggaggg catcgagggt cgcgtcgcct acaaaggggc cgtttccgaa acgatatatc
      181 agttggcggg cgggctccgg tcggggctcg gctatgtcgg ggcgaagaac ctcaccgaac
      241 tgcgcgagaa ggcccggttc atccgcatca cccccgccgg gttcaaggaa tcgcatgtcc
      301 acgatgtcac gataaccaag gaatcaccga actaccgcgc cgagcactga gcccggtcgc
      361 tactcatccg gacgtcggaa gaactccacc agatcgagca ggaaataggt atcgcgcgcg
      421 gcatcttccg gttcgagccg catgaagcgg aacccctccg ctacggtcgc ctcgacctga
      481 cggatgacct tctcgtaccg ttcgccggtg aagtcgacgc ggccgatctc gcggtcgtcg
      541 atgaacaccc ggaaggcccc catctcgggg ccggtcaccc cgacgacatc gatggagtag
      601 cgccccgcga gaaagaactg ctggccgaac tcgtagaatt gccccggtgc ggtgaaccgg
      661 cagaaaaagg cccgctttga gaacccgacc ccgatgcgtt cgggcggcgg gaaggagaca
      721 tattttgaga ggtcgctccg gttcgggtat tcgttgcagt aatcggggac gccgcggagc
      781 gggtagtgct tgtcctccat ctcggcgacg agatagttcg gcggcaggtc gggtcccgcc
      841 agcggcgtga gcaggggggg caccgtcgcg gtccgtatgt cggtgaaaag ggcgcggtag
      901 aatttcctcc cctgcggcgc gtagtggtgc atgagggcgc tgttcgggcg gtcgccgagg
      961 tcgcggacat agacgatgtc gttgcggtcc cagtcgtcat ggcgcatcgc gacgagcccc
     1021 gagccgatgt actgctcggg ctgtatgaag acggccgcgt tgtggatgcc ctgtcgcgcc
     1081 gcctcgtcgg cgagcgcgcg gccgacgccc cagaacccga aagcgtagga atcgaagagc
     1141 ggcgggagta tcacgacggc ggtgaacagg tatgcggcgc cgataaagcc gccgagcggc
     1201 gcggcgaaga ggtgcggtcg gggcgcggtg gcgatgcggt cggccagcga gaccagaccg
     1261 agcgcgacga gtatcatgat gaaaaaggtc gtttcatagt agtaccgcgg cccgaagaca
     1321 ttgccgtcga aatagtaaag gaaatggccg ccgatggtcg cgaggaacgg cgcggcgagg
     1381 aacatctccc gccggagccg accgccgcgc gcgaaaagga aggcgcaggg gatgagcagg
     1441 aacaggagcg ggtggccgag cgtgttggcg atgagcggcg cgagccggtg gtaggtcacg
     1501 acgaccgcgg cggtgagggt gaacccgtcg tcggggagcg gccgccaggt cgcgttggga
     1561 caggcgcggc cgagcccgag cccgaaacag tcgtcgttcg gctcgctgta gttgaaatag
     1621 atatcctgcc ggagcgtcag gaggtctccg gtgtagtggg cgttatggag gagcaggaca
     1681 agaagccacg gcaggaacgc gttctgcggg cggcagaagg cgagccagcc gagcgagaac
     1741 ccggcgaccg cggcgaagat gagcgcccgt ttcccgctcc cttcgcggcg ggcccgcagg
     1801 aagaacagga cggtggtcag cgtcaggaag gcgcagaacg ggtgcgccat cgcggtgccc
     1861 cccatgatca tgaggaaggg tgaaaggagc gcgagcgcca tcgcggcggt cgagacgcgc
     1921 gggccgtaga ggtcgtcggc gatgcgcccg gcgatcgcga cattggcggc ggtgagcagc
     1981 gggttggcga ggaacgcgac gccgaaaagg gcgaacggcg cgaggaaaag cgggtatccg
     2041 gggagaaaga gcgaggctat ccgttcgccg tcggggatga ggtagaggaa gcggaagaat
     2101 tcgtagtgcg ggtgcatcgc atggtccatc cgaccgtgcg ccagttggtc ggccatcatg
     2161 cggtagtgga tgccgtcctg tatgtggggg agcccctgaa aaacgaaaag cccgatgagg
     2221 agcgtcgcgg cgaaggcggc gagcgggaac agaagatagc ggcgcgcgac ggcgacgtcg
     2281 cggagcggcg ggagtatgcg gcgcagaggg gcgcggaaca acagcaggac gaagacgatc
     2341 cccgcgagaa gaagatgaaa ggagggagca tagaccgcaa gcggcgtcca gaaccgcccg
     2401 gcggtgcccc agacgaagaa gagcaggacg aaaaaggcgg cgcagaaaag ccgcataaga
     2461 tcaccggggg accctttcat gacgccatca taccaaagag cggccgaagg tcaatcatcc
     2521 ccgcattgct ttttgaccgt cgaggggtag agtggcgtga cgggaacgaa accggccgtt
     2581 acgcggtcta ttaagggaac tgtccgggca cgaggcgtat gagtaccttt cgcgcatacc
     2641 gattcaccga ggtcttcctt gtggcgggct tcatcttcgt cggatcgttc ttcgccgctt
     2701 ctgacgcccg ggccctcttc ggcgcgcggc cggcgctctt tttcctcgcg tcgtacctcg
     2761 tgacgctcgc ggtctacgcc ttcaacagtt gggccgggtc ttccgacgac gccgccaacc
     2821 cgcggctcgc ggtcgtgccg gacgaccgct ctccggcggt ggcggccgcg tcgcttatcg
     2881 ccgcgctcgc gctcttcctg atcgtggagc ggcgcgccgc accctgcgcg gcggcgctct
     2941 ttgtcctgtc ggcgctctat tcctatcccc gcttcggggc gaaatacctc ccgcgcgccg
     3001 ggacggcgct ccacatcctc ggcggcgcgg tccagttcca gatcgggtgg ctcatgttcg
     3061 gggagatcgg ggtccgctcg cttgcgctcg ccctctattt cgggctcatc ctgagcgccg
     3121 gtcatgtcaa ccacgaactg atagacctcg atgccgaccg cgcggcgggg atccggagcg
     3181 gcgcggtccg gtacggcgcg cccggctcga tacggctcca catcgtcatc tgcgccgccg
     3241 cgctcgcgct gctcatcgtt gcggcgctcc gcggcatggc gacgcccgtc caccttgcgc
     3301 cgttcgccgt ggcgtcgctc ctccagatgg cgtcggcggt cgcgctgtcg ctccgcgcgg
     3361 cgccggggca gggcgatttc ctgcggcacc ggcggctgta ccgtatcctg tacgctttgg
     3421 cggggatcgt ttttctcgcg gtgaagttcg gcccgtcact cggggcgtga cgcggcgtcg
     3481 gtgaagaact cgacaagatc gatcagaaaa tagacatcgc ggccggtcgt ttccggttcc
     3541 agccgtatgc ggtgggaccc ggcggcgagt tcggcctcca cgcggcgggt caccttttcg
     3601 taccgttcgc cggtgaagtc gacgatgcct atctcgcgcc cgtcgacgaa tgcccggaac
     3661 cgtcccatct cggggcccga caccccgacg aattcgaccg aatacctccc cgcgaccgca
     3721 aggtgctggc cgaggtcgta gaactggccc ggtgcggtga accggcagaa gagcgccccc
     3781 ttcgaaaaac cgatacccag ttcgccgggg ggcggcaggg cgctgtagcg gtcgaggtcc
     3841 gggcggttgg ggtaccggtt gcagtagtcg ggcgcgccgg tgagcgggta gagtttgtcc
     3901 tcgagttcgg cgacgacata gttcgggggc agttcgatgt cggtcagcgg ggttatctcg
     3961 ggcggccgct cgttgttctc tatcttttcg tagcgggcgc ggtagaactt tcgtccctgc
     4021 gccgcgtagt agtgcatgag cgcgctgttc gggcggacgc cgagatcgcg gacataaatg
     4081 acatcgttcc ggtcccagtc gtcgtgccgc atcacggcga agcccgaccc gatgagttcc
     4141 tcgcggtcta tgaagacgac ggcgttatgg atgccctgcc gttcgacctc cgccgagagc
     4201 gaccgctcga ccttccagaa gccgagcgaa tagatggtga gcaggtcggg gagcacgatg
     4261 aaggcgaacg agagataggc ggcggcgaca agcccgccaa gcggcgcggc gaagaggtgc
     4321 gaccggggcg cagcggcgat gcggtcggaa agcgcgacga gcccgagcgc ggcgagtatc
     4381 gcgaggaaga aggtcgtttc atagtagtag cgcggtccga agacattgcc gtcgaaatag
     4441 aagaagaaat aaccgccggc cgaaaagagg aacagtgcgg cgaggaacag ttcctttttc
     4501 ggccgcccgg accggtagaa gaggaaggcg caggggatga gcaggaacag gagcgggtgg
     4561 ccgaaggtgt tgaggatgag cggcgagagg cggcggtagg tgacataggc ggcgtgcccc
     4621 caggtgagcc cctcttcggg gagcaccgtc cagttcgaat ggggacagcc gcggccgatg
     4681 ccgaaacggt ggcagaactc gttcggctcg ctgtagttga aataggggtc ctgcttgaac
     4741 aggagcgggt cgccggtgta gtgggcgttg tataaaagga gcgcgataag ccacggtagg
     4801 aaggcggcgg tgaggactag tccgcgcgcc accgtccccc ggcggcgtat ctcgaaaagc
     4861 gcggcaagcg caagcggcaa agcgagaaag agcgcgtttt gcggtcgggt gaatacgagc
     4921 cagccgagcg cgaagccggc ggcggcagcg tagaggagcg ccgcttttcc gctccccgcg
     4981 cggcgggccc gcaggaagca gaggacggcg ccgagcgtca gggcggcgca gaaagggtgc
     5041 gccatgaagg tgccgcccat caccatgagg aataccgaaa ggatcgcgag cgccatcgcg
     5101 gcggccgaaa cgcgcgggcc gtagaggtcg tcggcgatgc ggcccagcag gaagatgttt
     5161 gcggcggtca ggagcgggtt gacgatgaag gggatgccga tcatcgcgaa ggggacgaga
     5221 aagagcgaat agccggggag gaacagcgaa tagatcttct cgccgtccgg tatgagatag
     5281 aggaaccgga agaactcgta gtaggggtgc atcgggagat cgagccgccc cgccgcgaac
     5341 tgctcggcca tgaacaggta gttgatgctg tcctgtatgt gggggaggcc ctgaaagacg
     5401 agtcggttga tgaggagcgt cacgcagaag gcgagaagcg ggaagaggat atagcggcgc
     5461 ggcacgatgc gggcggagag cgaccgggcg gcggcgcgga taggcggcag gaggaagagg
     5521 gccgtcaacc cggcgaagag cagatagaac cacgggacat agagttggaa cgggcgccag
     5581 aacttcccgg cggtgcccca gacgaagaag aggagcgtga aaaaaagcgc ggcgaccgcg
     5641 cggaccatgt cgcaccgact gaacttcatg acgacatcat agcgaaccgc gcggagaggt
     5701 caacgataac ttaccgctgg gtgacgatgc gtcccaacgg ggtcaccaac ggggtcacac
     5761 ctaaaattta ataggttttg ctttctcgtt cctgctaacc accgctatcg gtcgctgatc
     5821 cgtcgcacaa gaaattcttg attcgcaacc cggataaaag taacatcgcg aggcaacagg
     5881 ggaggttggt tcggtgggga acgaatcgcg aaatatttta aaacatcaat gtggttccaa
     5941 tgtaatcatc cttgtggcgg cgctgatcac ggcggcttgc ggtagcggga caaaagcatc
     6001 ttctgatgat actaacggaa tgagttggca aagaatctct acgggcgggc aacacacctg
     6061 tgccatacaa ggtgacggca agcttttctg ttggggacac aattcctgcg gacaactcgg
     6121 cgatggaacc acggaagaca agaacacccc gactcatgtc ggctccgaag atgattggaa
     6181 gagcatctcc gtcggcgaag aacatacctg tggcgtgaac gggcgtggag aactgtattg
     6241 ttggggaaac aacgaccatc aacagatcgg tgatggtact tttagagatg caaatagtcc
     6301 tgttcgtatc ggtaccaatg gtgactggca gaatgtctcc gccggtgcgc gccacacctg
     6361 tgcggtgaac atgagcggtg aactctactg ttggggagac ggtgaagccg gtcaagtggg
     6421 tgatggcgcg aacgaaatga acagaagcgc cccgactcgt gtcggaacgg agagcgattg
     6481 gcagaaggtt tccgccggta ttgctcacac ctgtgccgta aaggtaagcg gcgaacttta
     6541 ctgctgggga agtaatctat acgggcaact cggtgatgaa actacagaat caaaaaacac
     6601 gccggtccac atcggcgtaa aaagcgactg gcaaagtgtc tccaccggtg aaaaacacac
     6661 ctgcggcctg aaaaggtccg gcgaactcta ctgctgggga aacaatcaaa agggacaact
     6721 cggtgatgaa actacagaat cgaaaagtac gccggtccac ataggcgtaa agagcgagtg
     6781 gatgagtgtc tctgccggtt tgagccacac ctgcgccgta acaatgagcg gcgaacctta
     6841 ctgctggggg cagaactggg atggacagct gggcgacggg acaagggaag ataaaaacac
     6901 gccgacccgg attgactttg taggagaatg gaagagcatc tccgccggat ggaaacactc
     6961 ttgtggcgag agaagagacg gatggctgct ttgctggggg gaaaatcagt ctgggcaact
     7021 tggtaaagga gccttccctg ataagaacat tcctacccac atcggtgccg caagcgactg
     7081 gcagggcgtt tctgccggag acggacatac ctgtggcgtg aaaaagtccg gcgaactcta
     7141 ttgctggggg aacgccgcgc acggtccctt cacagaaatt acgctacctc aagacatccc
     7201 aaaatctttc gatgtaaaaa gcgattggca gagcatctcg gccggcatgt atcacacctg
     7261 tggcgtgaaa aactccggcg aactctactg ctggggagac aaccgattcg gtcaactcgg
     7321 tgatgggacc gaggaaaaca aaggtgcccc ggtccgcatc ggcatggata gcgattggca
     7381 aagcgtctcg gcccgcacat ggcacacctg tggcgtgaag aaaacgggca aactgtattg
     7441 ttgggggtgg aacgagtacg gtcggcttgg cgacggatcc gaattcacca gaaacacgcc
     7501 gactcgcatc ggcaccgcaa tcgactggga tgtcgtctct gccggcgaag catactcctg
     7561 tgccctgaaa acgaacggcg aactgtactg ttggggacac aatttccatg gacaactcgg
     7621 cgatggaacc acggaagaca ggaatacgcc gacccgtatc ggcatggata gtgactggga
     7681 aagcgtttct gccggcgaaa gatactcctg tgccttaaaa agcagcggcg aactgtactg
     7741 ttggggtaat gacgaatccg ggttaccaga catagaggac acaggaagca ccaccccggt
     7801 ccgtgtcggt accgcaagag attggaagag tatctctgcc ggtatgtatc acacctgcgg
     7861 cgtgaaggga atcggcgaac tttattgttg gggagagaac tatttcggcc aactggggga
     7921 cgggacgcgg gaatacaaag atgttccaat ccgtatcggt gcggaaagcg tttggcagag
     7981 tgtctccgca ggcacctatc acacttgcgg catacagggc actgctgaac tgtactgttg
     8041 gggaaggaac gaatacggcc agttaggtga cgggagtatg tgggaggaaa cccctcaaca
     8101 agttgaagtc aattagcggg atcatagagg tcgcataaat cttaatagtt ctgcttctct
     8161 gattatccct acccgttccg cgtgataatg cgtcccgccg cgatgataag aatgccaccg
     8221 ccgatgataa agatattgag tggcagtgag aggaacagga gttcgttcag cgcggtgaag
     8281 agtatcgtga agaggaacag caccgacgcg gtgttgaccg gaatgtcgcg cagggcgtag
     8341 ttccagcaga gataggcgcc gcccgactgg aagaccccca tctcgacgag ccagaaccat
     8401 tcatcggcct tctgcggccg tggacagtcg cgcactaaaa acgggatcat cagcaccgtc
     8461 gaaatgacga atatcttgaa caggaactgc accaccttca ccggatcgtc gcgcagaggt
     8521 cgcaccaact cggccgaaaa gtaggcgtac cagccgaagg tcgccccggc gagcaccgcc
     8581 cagatgatgc cgacattcac atcgccgccg ccgaaatcga aggtgtcctt gccgtagacg
     8641 atgaagagca tcccgcacag tgataacagc agggcgatca cctcgcgcca cgtcggccgc
     8701 accccggcga gcgtgaacga gaggaagaag acgaacagcg gcgagagatt ctcgatgatc
     8761 atcacgagcg ttcccgatat cgagttcagc gcgagatgga aggtgatggt gttgaggaac
     8821 aaacccacga acgagagcgc cgcgacggtg cgccaccgtt cgcgccagtc catcgtgaag
     8881 tttccggtgg cgatgagaaa gacgccgaag atgatcgttc ccatca
//
LOCUS       DAOQUE010000051         8882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8746_length_8882_cov_4.075790, whole genome shotgun sequence.
ACCESSION   DAOQUE010000051 DAOQUE010000000
VERSION     DAOQUE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8746_length_8882_cov_4.075790"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PKH10_02640"
     CDS             complement(<1..856)
                     /locus_tag="PKH10_02640"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNT27053.1"
                     /translation="MNRKNLVLLAILALLIGWFLLKKHLDEQAIHRNIEQENRAVFEG
                     LAKEQITEVAITDTEKKITTTLAKQGDRWVVKEKNNFPADKALVDKVLETLPKFRYGT
                     QIGDQEEMPKTSYGFDKAVEVKVAGKTLVLGNPAGARLPIKVDGALYLSPSNERHVFA
                     RYDGEWRERKLFPGREPADVVGLKITLEGRAPVEVKLGENDEATVTGLDGADPQKAKS
                     LLSMVGGLRIGSFFDAPPPMTDEVEKDKKKEKIAKGTVTVSFKNGEAATFELVGQKVK
                     DKSDYLISK"
     gene            complement(858..2468)
                     /locus_tag="PKH10_02645"
     CDS             complement(858..2468)
                     /locus_tag="PKH10_02645"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNT27054.1"
                     /translation="MSKKKLDALIAIALVAGIALFVNILASYAFLRLDLTRGGIYTVS
                     DSTKKILADLPGTVQVKFYLSDELPPRVLPVKRDILDILKEYENYGKGKVELSVLVPE
                     KDPNIEEDAKKAGIEKVNINVIGKNKEEVQAVYLGIALYYQEKSEILPVVMGIKDIEY
                     ELTSAILKLTREKKDTIVFLNKEVRLPDDLDPQMRMQLQSQLPPSHSITKDTMAVAEA
                     LREQYEVREVQIKEGDPLPDGTTVLVIHEADNLTEWEKYLVDQFVLKGGSLVVLQSGI
                     KFQQGMMGQERRINYGNLLEAWGLKLQGNMVLDLYNFPALIPSGNMRYLQPYPFWIKV
                     GAKQMDAALPGYLRELGTLAFPFSSSIEIEQKEGLSYATVARSSGKSWEQRGAVMAMP
                     DQITQPQESEFKTFDLAVLASGTFPSAFTSGNLPPKADAASFLAKGEKPGRVFLAGTP
                     ELILDRNMRMFQSNGIFFINLVDYLTNSSDLAGIRSRDQGYVFIDAEVSDAMKEIIRW
                     VGILLMPLVVVIFGVIRMMMRNRLSGRA"
     gene            complement(2481..3194)
                     /locus_tag="PKH10_02650"
     CDS             complement(2481..3194)
                     /locus_tag="PKH10_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT27055.1"
                     /translation="MNNILTVTRKELLGYFNSAIAYIFFTLFLVILSFLFFRLFFLQG
                     IVSVNDFFGVMPWVFLIFVPAVTMKSWAEERKSGTMEVLLSLPVSDLELVVGKFLAGV
                     AFVGVALLLTLIVPTLVSLTGPLDWGPVVASYLGAILLAAAYVAIGNFVSSLTANQIT
                     AFLFTAVLLFLLLIIGTEPVFSVFPTLIGEVVRNFSLNYHFQSIGRGVIDSRDILFYL
                     TVIGLFVYYNIKSLEARSW"
     gene            complement(3191..4126)
                     /locus_tag="PKH10_02655"
     CDS             complement(3191..4126)
                     /locus_tag="PKH10_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT27056.1"
                     /translation="MTEMITVRGVTKRYDRITAVADASFTLRKGDILGFLGPNGAGKT
                     TAMKMIIGYLPADTGTIAVAGRDITEDPVKARNAIGYLPENNPLYQDMTVDEYLSFIA
                     AMRGLKDPAAAKKRAMERCAVDAVGHRIIGHLSKGYKQRVGLAQAILHDPEILILDEP
                     ANGLDPIQITEIRGLIRDLGREKSVILCSHILSEVEAVASRVVIINQGKIVADDTLDN
                     LKERSSGHNIFRVEFLTAKDERTVADALTKIPGVAAVSRVAGDTFRVKTKGERETGNQ
                     IFRAAVAQNWDLAQIHLESRSLENTFLSLIEGGNA"
     gene            complement(4186..4977)
                     /gene="panB"
                     /locus_tag="PKH10_02660"
     CDS             complement(4186..4977)
                     /gene="panB"
                     /locus_tag="PKH10_02660"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNT27057.1"
                     /translation="MKKMTLPALRDKKGKDPIAMVTCYDAAFARIAEAAGIETLLVGD
                     SLGMVIKGEESTLGVSVDEVAYHVRAVRRGAPAPFLIADMPFGSYQASVAQGVDNAVT
                     LMKAGAEAVKVEGGVAVVPLVERLTAVGIPVVGHVGLTPQSVNLFGGYRKRGITKREH
                     DEILNGALALDAAGAALIVLEMVPAELAARITAEVSAPTIGIGAGPDTDGQVLVLYDL
                     IGMDGSFNPSFLKKYLDLDRQVTDALTRYRDEVRTGRFPARRNKS"
     gene            complement(4974..5642)
                     /locus_tag="PKH10_02665"
     CDS             complement(4974..5642)
                     /locus_tag="PKH10_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27058.1"
                     /translation="MIRLLALIALCLSCAACSSEPAPAWQNEGVAQLANFMQRYLEGD
                     DRLAGIHFEKALEHISKTGDPTLVGRLYLMRCALGVASLIDTPCEEATRYAGDPANDA
                     YRAQMAGGAVDRKALPPQYRDLDAAFVERSVAKVNAALGDIDEPLSRCIGAGLAVSRG
                     LFDAATLELAARAASEQGWKRPLTAYLTKLRDLYAAQGDRQKETETQRRLDIVSGTGG
                     EKSE"
     gene            complement(5639..6583)
                     /locus_tag="PKH10_02670"
     CDS             complement(5639..6583)
                     /locus_tag="PKH10_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNT27059.1"
                     /translation="MARDTLQYKVGLFIVSTVALILATAVLVAYKKDLFADLATYRLL
                     SESGEGLSQGMPVLFSGFEIGKVSALELSEKGEVLITISVAQKHARWVRETSAFTLEK
                     PLVGSPRIVVKTEDTGSAELPADKIVPIKTIDDINEVIKKAQPLVERIQSVADNVDKM
                     TNEGSELNKIIADTRKITKMLSEKKSLLEMATGDKKGQETLMKTLEQVAAITATVDTT
                     VKRIDSMTAKADEGVFGDKGSLATVNNILLDIEAKFKRLDALIDNAVTMSADLQGTTQ
                     DINLLRKDVDETVSSLRQLLQDVRNSLPFDQKKEIKLP"
     gene            complement(6573..6995)
                     /locus_tag="PKH10_02675"
     CDS             complement(6573..6995)
                     /locus_tag="PKH10_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27060.1"
                     /translation="MMELATAEKGRYAPVSADIPLLSNLSVFRNIALIRQYHERLGRE
                     ESHRLTTEALERFGLCGIAEKRPYDLSERDLFLVKLLRAAQRKEALIVIDRPFVQIAG
                     LRSFEWIRKHLELLDFPGIDAIILDYEWNGERYGSDGA"
     gene            complement(7031..7822)
                     /locus_tag="PKH10_02680"
     CDS             complement(7031..7822)
                     /locus_tag="PKH10_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT27061.1"
                     /translation="MNAVSWIESVGAGALGRWRELHQLLGFASAVFRRLLSPSSYRPL
                     TVEIAVTQIYFTAVQLLGFFIVVSVVFGSLFIGMTLFVIKEIGLTGYIGDFIAGVVVL
                     EMAPIMTTLLIALRSGSAINAEMAVMKANRELDALEAFGVDIANYLFLPRIVAVMVSV
                     VLLSALFAIIVTASGLVFSAIIFDMSFNTYTELIAKAIEYRYFTIMVVKSAAFGFFIT
                     FIPLYYGSRAGYQLTAVPVAVLRGMVNVFIAIIIIEVLSLVARFL"
     gene            complement(7929..>8882)
                     /locus_tag="PKH10_02685"
     CDS             complement(7929..>8882)
                     /locus_tag="PKH10_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27062.1"
                     /translation="LGIPYEIDGINFHATVTVEAGTTIAVMPNEIVNVSENGALIMIG
                     TVAQRITVTSAKTTPKAGDWDYIGIYNTASEDNRFEYVDFKYGGSTYGIFWVEQGASV
                     SFDNCLFSDITGPGMEMKDVTLGSFTGNSFKKISEYPLVVTASVVPSLSPITTSDLTA
                     GKDRVHVVGGYTEVGGLWKELGIPYELEGLSFRESVTVEAGTTILIEPNEIINIAEGG
                     SLILDGTADKRITVKSSKTTPAAGDWNYFGFYNTSSNDNRFSYADIMHGGSGNYGQIW
                     VEQDATLTLEHVVFSEGDTCDIDADEDAILNVTDTTFVACP"
BASE COUNT         1559 a         2680 c         2822 g         1821 t
ORIGIN      
        1 ccttgctgat gagatagtcg ctcttgtcct tcaccttctg gccgaccagt tcgaaggtgg
       61 cggcctcacc gttcttgaac gagacggtga cggtcccctt ggctatcttt tcttttttct
      121 tgtccttttc cacctcgtcg gtcatcggcg gcggtgcgtc gaagaacgac ccgatacgga
      181 gcccgccgac catcgagagg agcgacttcg ctttctgcgg atcggccccg tcaagaccgg
      241 tcaccgtcgc ctcgtcgttc tcgcccagtt tcacctcgac gggggcgcgt ccttcgagcg
      301 ttatcttgag accgacgaca tcggccggtt ccctgcccgg gaacagtttc cgttcgcgcc
      361 attcgccgtc gtagcgggcg aagacatgcc gttcgttgct cggcgaaagg tagagagcgc
      421 cgtcaacctt gatggggagc cgcgccccgg cggggttgcc cagaacgagc gtcttgcccg
      481 ccaccttcac ctcgaccgcc ttatcgaagc cgtaggaggt cttcggcatc tcctcctggt
      541 cgccgatctg ggtgccgtaa cggaatttcg gcagggtctc cagcaccttg tcgacgagcg
      601 ccttgtcggc cgggaagttg ttcttctctt tgaccaccca ccggtcgccc tgtttggcga
      661 gggtggtggt gatcttcttc tcggtatcgg tgatcgcgac ctccgttatc tgctctttcg
      721 cgagtccctc gaagacggcg cggttctcct gttcgatgtt ccggtgtatc gcctgctcgt
      781 cgagatgttt tttcagcagg aaccagccga tgagcagagc cagtatagcc agcagaacga
      841 gatttttcct gttcatgtca cgctctccct gaaagtctgt tcctcatcat catccggatg
      901 acaccgaata tcacgaccac gagcggcatc agcaggatac cgacccagcg gatgatctct
      961 ttcatcgcat cggatacctc ggcgtcgatg aatacatagc cctgatcgcg cgaccggata
     1021 ccggcaaggt cgctcgaatt ggtgagatag tccacgaggt tgatgaagaa tatcccgttc
     1081 gactggaaca tccgcatgtt gcggtcgagt atcagttcgg gggtgccggc gagaaagacc
     1141 cgccccggct tttctccctt cgcgaggaag gaggccgcat cggccttcgg cggcaggttg
     1201 ccgctggtga aggcgctcgg gaaggtgccc gatgcgagca ccgcgagatc gaaggtcttg
     1261 aactcgcttt cctgcggttg cgtgatctga tcgggcatcg ccatcaccgc tccccgctgt
     1321 tcccaactct tgcccgatga acgggcgacc gtcgcgtagg agagcccttc tttctgctct
     1381 atctctatcg aggaggagaa cgggaaggcg agcgtaccga gttcgcgcag gtagcccggg
     1441 agcgcggcgt ccatctgctt cgcgccgacc tttatccaga aggggtaggg ctgcaggtac
     1501 cgcatgttac ccgacgggat gagcgccggg aagttgtaaa ggtcgagcac catgttgccc
     1561 tgcagtttga gtccccacgc ctcgagcagg ttgccgtagt tgatgcgccg ctcctgcccc
     1621 atcatcccct gctggaactt gatgcccgac tgcaggacga cgagcgaccc gcccttcagc
     1681 acgaactggt cgacgaggta tttctcccat tcggtcagat tgtcggcctc gtgtatcacg
     1741 agcaccgtgg tcccgtcggg cagggggtct ccctctttta tctggacctc gcgcacctcg
     1801 tactgctcgc ggagcgcctc ggcgaccgcc atcgtgtcct tcgtgatgga gtggctcggc
     1861 ggcaactggc tctgcaactg catccgcatc tgcgggtcga ggtcgtcggg gagccggacc
     1921 tccttgttga ggaagacgat ggtgtccttt ttctcgcgcg tcagtttgag tatggcgctc
     1981 gtcagttcgt actcgatgtc cttgatcccc atgacgaccg gcaggatctc gcttttttcc
     2041 tgatagtaca gcgcgatgcc gagatagacc gcctgcacct cctccttgtt cttgccgatg
     2101 acattgatgt tgaccttctc tatgcccgcc ttcttggcgt cctcctcgat gttcggatcc
     2161 ttttcgggaa cgagcaccga caattccacc ttgcccttgc cgtaattctc gtactccttg
     2221 aggatgtcga gtatgtcgcg tttgaccggc aggacgcgcg gcggcaactc gtcggaaaga
     2281 tagaatttca cctgcaccgt tcccggcaga tcggcgagta tcttcttggt cgagtcgctc
     2341 acggtgtaga tgccgccccg cgtgagatcg agccgcagga aggcgtagga ggcgagtatg
     2401 ttgacgaaca gcgctatgcc ggcgacgagt gcgatggcga tgagggcgtc cagtttcttt
     2461 ttactcatgg cgtcacctcc ctaccagctg cgcgcttcga gcgacttgat gttgtaatac
     2521 acgaacagcc cgatcaccgt gaggtagaac aggatgtcgc gcgaatcgat gacgccgcgc
     2581 ccgatgctct ggaaatgata gttcagcgag aagttgcgca ccacctcgcc gatgagggtc
     2641 gggaacaccg agaataccgg ctcggtcccg atgatgagca ggaggaacag gaggaccgcg
     2701 gtgaagagga acgccgttat ctgattggcg gtgagcgaac tcacgaaatt cccgatcgcg
     2761 acataggcgg cggcgagcag gatcgcgccg aggtacgacg cgacgaccgg gccccagtcg
     2821 agcgggccgg tgagcgacac gagcgtcggc acgatgagcg tgagcaggag cgcgacaccg
     2881 acgaacgcga cgccggcgag gaacttgccg acgaccagtt cgaggtccga caccgggagc
     2941 gacagaagca cctccatggt gccgctcttg cgctcttcgg cccacgattt catggtgacc
     3001 gccgggacga agatgagaaa gacccacggc atcacgccga agaaatcgtt caccgagacg
     3061 atgccctgca ggaagaacag gcggaagaac aggaacgaga gtatgacgag gaacagcgtg
     3121 aagaatatgt aggcgatggc cgagttgaag tagccgagca attccttgcg ggtgacggtc
     3181 agtatgttgt tcatgcgttg cctccttcga tgagtgacag gaaggtgttc tcgagagaac
     3241 gcgattcgag atggatctgc gcgagatccc aattctgcgc cacggcggcc cggaatatct
     3301 ggttgcccgt ctcgcgttcg cctttggtct tgacgcggaa ggtgtcgccg gccacgcggc
     3361 tgaccgccgc gacgcccggt atcttcgtca gcgcgtcggc gaccgtccgc tcgtccttcg
     3421 ccgtcaggaa ctcgacgcgg aagatgttgt ggccgctgct gcgctccttc aggttgtcga
     3481 gcgtgtcgtc ggcgacgatc ttcccctgat tgatgatgac cacgcggctc gcgaccgcct
     3541 cgacctccga gaggatgtgc gagcagagga tgacgctctt ctcgcggccc agatcgcgga
     3601 tgagcccgcg gatctcggtt atctgtatcg ggtcgagccc gttggccggt tcgtcgagta
     3661 tcaggatctc cggatcgtgc aggatggcct gcgcgaggcc gacgcgctgt ttgtaacctt
     3721 tcgagagatg cccgatgatg cggtggccca ccgcgtcgac cgcgcaccgc tccatcgccc
     3781 gtttcttcgc ggcggccggg tccttgagcc cgcgcatcgc ggcgatgaac gacagatact
     3841 cgtcaacggt catatcctga tagagcggat tgttctccgg cagatacccg atggcgttgc
     3901 gcgccttcac cgggtcctcg gtgatgtcgc gcccggcgac cgcgatggtg ccggtatcgg
     3961 cgggcagata gccgatgatc atcttcatcg cggtggtctt gccggcgccg ttcggaccga
     4021 ggaagccgag gatgtccccc ttgcggagcg tgaacgaggc gtcggcgacc gccgtgatgc
     4081 gatcgtaacg cttggtcacc ccgcgcaccg tgatcatctc agtcatgcga aaacctcctt
     4141 tttatgaata catacagcat agtacgccac ttaaacaacg aggcgtcagg atttattccg
     4201 ccgcgccggg aaccggccgg tccgcacctc gtcgcggtat cgcgtaagcg cgtcggtgac
     4261 ctgacggtcg aggtcgaggt attttttgag gaacgaggga ttgaacgatc cgtccatgcc
     4321 gatgaggtca tacaggacaa gcacctgtcc gtcggtgtcg gggcccgccc cgatgccgat
     4381 ggtgggggcg gacacctcgg ccgttatccg cgccgcgagt tcggcaggga ccatctcgag
     4441 cacgatgagc gcggcgcccg ccgcgtcgag cgcaagcgcc ccgttcagga tctcgtcgtg
     4501 ttcgcgcttc gttatgcccc gtttgcgata gccgccgaaa aggttcaccg actgcggggt
     4561 gagccccaca tggccgacga ccgggatgcc gaccgcggtg agccgttcga ccagcggcac
     4621 gaccgcgaca ccgccttcga ccttgaccgc ctcggcgccc gccttcatga gcgtgacggc
     4681 gttatcgacc ccctgcgcga ccgacgcctg ataggagccg aacggcatgt cggcgatgag
     4741 gaacggcgcc ggggcgccgc gccgcaccgc gcggacatgg taggcgacct cgtccaccga
     4801 gacgccgagc gtactttctt cgccctttat caccatgccg agactgtcgc cgaccagcag
     4861 ggtctcgatg ccggccgcct cggcgatccg cgcgaacgcg gcgtcgtagc aggtcaccat
     4921 cgcgatgggg tccttccctt ttttgtcccg cagggcgggg agcgtcatct tcttcattcg
     4981 cttttctctc ctccggtccc cgaaacgatg tcgagccgtc gctgggtctc ggtctctttc
     5041 tgccggtcgc cctgcgccgc gtagaggtcg cgaagtttcg tgaggtacgc ggtgagcggt
     5101 cgtttccagc cctgctcgga ggcggcacgt gccgccagtt cgagcgtcgc ggcgtcgaac
     5161 aggccgcgcg acaccgcaag ccccgcgccg atgcagcgcg agagcggttc gtcgatgtcg
     5221 ccgagcgccg cgttgacctt ggcgaccgac cgctcgacga aggccgcgtc gagatcgcgg
     5281 tactgcggcg gcagggcctt ccggtccacc gcgccgcccg ccatctgggc gcgataggcg
     5341 tcgttggcgg ggtcgccggc gtagcgggtc gcctcctcgc acggggtgtc gatgagcgac
     5401 gcgacgccga gcgcacaacg catcaggtac agtcgcccca caagcgtcgg gtcgccggtc
     5461 ttggagatat gttcgagcgc cttttcgaaa tggatgccgg cgagccggtc gtccccttcg
     5521 agatagcgtt gcatgaaatt ggcgagttgc gcgaccccct cgttctgcca cgccggggcc
     5581 ggctccgacg agcaggcggc acaggagaga cacagcgcga tgagggcgag taggcgtatc
     5641 atgggagttt tatctccttt ttctggtcga acggcagact gttgcgaaca tcctgcagga
     5701 gttgccggag cgaggagacg gtctcgtcca catccttgcg caggagattg atgtcctgcg
     5761 tcgttccctg caggtcggcg ctcatggtca ccgcattgtc gatgagcgcg tcgagccgct
     5821 tgaacttcgc ctcgatgtcg agcaggatgt tgttgacggt cgcgagcgag cccttgtcgc
     5881 cgaacacccc ctcgtcggcc ttcgcggtca tggagtctat ccgcttgacg gtggtatcga
     5941 ccgtcgcggt gatcgcggcg acctgttcga gcgttttcat gagcgtttcc tgtcccttct
     6001 tgtcgcccgt tgccatctcg aggagcgact ttttctccga aagcatcttc gttatcttgc
     6061 gggtgtcggc gatgatcttg ttgagttcgc tcccctcgtt ggtcatctta tcgacgttgt
     6121 cggcgaccga ctgtatccgc tcgacgagtg gctgggcctt cttgatgacc tcgttgatgt
     6181 cgtctatcgt ttttatcggg acgatcttgt cggcgggcag ttcggcgctc ccggtgtctt
     6241 cggtctttac gacgatgcgc ggactgccga cgagcggttt ttcgagcgtg aacgcactcg
     6301 tttcgcggac ccagcgggcg tgtttctggg ccaccgatat cgtgatgagc acctctcctt
     6361 tctccgacag ttcgagcgcc gagaccttgc cgatctcgaa gccggagaag aggaccggca
     6421 tcccctgcga caggccttcg cccgactccg acaggagccg gtaggtcgcc agatcggcga
     6481 acaggtcctt cttgtaggcg accagtacgg cggtggcgag gatgagcgcg acggtgctca
     6541 cgatgaagag tccgaccttg tattggaggg tgtcacgcgc catcgctacc gtaccgctcg
     6601 ccgttccatt cgtagtcgag gatgatagcg tcgatgccgg gaaagtcaag gagttccaaa
     6661 tgtttccgta tccattcgaa ggagcggagc cccgcgatct gcacgaaggg acggtcgatc
     6721 acgatgagcg cctccttccg ctgggcggcc cgcaggagtt tcacgaggaa caggtcccgc
     6781 tcgctcaggt cgtagggccg tttctcggcg atgccgcaca ggccgaaccg ttcgagcgcc
     6841 tcggtggtga gccggtgcga ctcctccctc ccgagccgct cgtggtattg gcggatgagc
     6901 gcgatattac ggaacaccga caggttggaa agaagcggga tgtcggccga taccggggca
     6961 tagcgcccct tttcggcggt cgcgagttcc atcatcgccg cgtcgagcgt cgcgtcgtcg
     7021 gcgaaggttc tcagagaaaa cgggcaacta atgacagcac ctcgatgatg atgatggcta
     7081 tgaacacatt gaccatgccg cgcaggaccg ccaccggcac cgccgtcaat tgatacccgg
     7141 cgcgcgagcc gtagtagagc gggataaagg tgatgaagaa cccgaacgcg gcgcttttga
     7201 cgaccatgat ggtgaagtaa cggtattcta tcgccttggc gatgagttcg gtgtacgtgt
     7261 tgaaactcat gtcgaagatg atcgccgaga acacgagccc gctcgcggtc acgatgatgg
     7321 cgaagagcgc cgacagcagg acgacgctta ccatcaccgc gacgatgcgc gggaggaaca
     7381 ggtagttcgc gatgtcgacg ccgaacgcct cgagcgcgtc gagttcgcgg ttggccttca
     7441 tcaccgccat ctcggcgttg atggccgacc cgctacgcag ggcgatgagg agcgtcgtca
     7501 tgatcggcgc catctcgagc accaccacgc cggcgatgaa gtcgccgatg tagccggtca
     7561 gcccgatctc cttgatgacg aacaatgtca tgccgatgaa gagcgagccg aagacgaccg
     7621 agacgacgat gaagaatccg agcagttgca cggcggtgaa gtatatctgt gtcacggcga
     7681 tctcgacggt gagcggccgg taactcgacg gcgagaggag ccgccggaac accgcggagg
     7741 cgaacccgag cagttggtgc agttcacgcc agcggccgag cgccccggcg ccaacgcttt
     7801 ctatccacga taccgcgttc atagccctcc cgggcgacat gccttccgaa cgagagggca
     7861 tactatcaga ggaagggcga ttcgtcaatg aaacggaggg aaggggagcg gaaggagacg
     7921 acgaccgcct aggggcaggc gacgaaggtc gtgtcggtca cattgagtat cgcgtcctcg
     7981 tcggcgtcga tgtcgcaggt gtcgccttcg gagaatacga catgttcgag cgtgagcgtc
     8041 gcgtcctgct cgacccagat ctggccgtag ttgcccgagc cgccgtgcat gatgtcggcg
     8101 taggagaacc ggttgtcgtt gctgctcgtg ttgtagaacc cgaaatagtt ccagtcgccc
     8161 gcggcgggcg tcgttttgct gctcttcacg gtgatgcgct tgtcggcggt gccgtcgagt
     8221 atcagcgagc cgccctcggc gatgttgatg atctcgttgg gttctatcag gatggtcgtc
     8281 cccgcctcga cggtgaccga ctctctgaaa ctaagtcctt cgagttcgta cgggataccg
     8341 agttccttcc agagaccgcc tacctcggtg tagccgccga cgacatggac gcggtccttg
     8401 ccggcggtca ggtcggaggt ggtgatgggc gacagggagg ggaccacgct ggcggtcacg
     8461 acgagcgggt attcggatat cttcttgaac gagttgccgg tgaaactgcc gagcgtgacg
     8521 tccttcatct ccatgccggg gccggtgatg tcgctgaaca ggcagttgtc gaaggagacc
     8581 gacgcgccct gctcgaccca gaatatgccg taagtgctgc cgccgtactt gaaatcgaca
     8641 tactcgaacc ggttgtcttc cgaagcggtg ttgtagatgc cgatgtaatc ccaatcaccc
     8701 gccttcgggg tggtcttggc gctcgtcacg gtgatgcgct gggcgacggt cccgatcatg
     8761 atgagcgcgc cgttctcgct gacattgacg atctcgttgg gcatgaccgc tatcgtggtc
     8821 cccgcctcga cggtgacggt ggcgtggaaa ttgatgccgt cgatctcgta ggggatgccg
     8881 ag
//
LOCUS       DAOQUE010000052         8835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8805_length_8835_cov_4.793736, whole genome shotgun sequence.
ACCESSION   DAOQUE010000052 DAOQUE010000000
VERSION     DAOQUE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8835
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8805_length_8835_cov_4.793736"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PKH10_02690"
     CDS             complement(<1..729)
                     /locus_tag="PKH10_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNT27063.1"
                     /translation="MKRTIFFIILLSIITYLNWDRIEPLLPLSAPAPHAVADFEAAKK
                     LLMEKVYYDHRETIYCGCPFDEKKQIDLDACGYTPRKDPQRAARLEWEHVVPAHAFGQ
                     SFAEWRDGHSDCADKKGRSFKGRRCARLTSNEFRRMEADMYNLYPAVGEVNGDRSNFS
                     MAEIPGEKREYGACDVEIEDRKIEPREAIRGLIARTYLYMDRAYPGRGVISDKNRKLF
                     DAWDALYPVTGWECERMRRSEAVQK"
     gene            complement(803..976)
                     /locus_tag="PKH10_02695"
     CDS             complement(803..976)
                     /locus_tag="PKH10_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27064.1"
                     /translation="MKNRIEKKRKITPKPTTPKCVEMMRAIRTALFADYLKNPALMKS
                     DLENIRKQYGLAS"
     gene            complement(966..1430)
                     /locus_tag="PKH10_02700"
     CDS             complement(966..1430)
                     /locus_tag="PKH10_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain protein"
                     /protein_id="HNT27065.1"
                     /translation="MKHGFRVYIDTSVFGGCSEKEFGVWSNALLSEISKGKKTAVISD
                     ITIKELSLAPREVRSVFEAIPEKHREIVALTKECEALAQEYIKEKAVSPKFEGDAYHI
                     AIATVCRADVLVSWNFKHMVNVRRIQRYNAVNLKHGYSMIDIRSPREVIDEE"
     gene            complement(1532..2743)
                     /locus_tag="PKH10_02705"
     CDS             complement(1532..2743)
                     /locus_tag="PKH10_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27066.1"
                     /translation="MNDLSDILEEFVCSEVVVIDKDGNKSACEGKIVCYNNGDAEFQT
                     RDCFNCSLVENARMTGKTKEGMAFEIRGVWCVDKFERHEVGNPVKYEARFSFEKAILT
                     ADDTLLPEFEDHVIVNLNTTGKREVRFTCGGKKCLLVFDHLRGIATLRADEQLGISSD
                     ILMLLSLAQRNVFFSPIRKTYNGTTLSKISIFPNKGLRSAHPMISRDAEKLAKFIENA
                     WPYYTKNQEEYNLPRLFQFYCLSIADEIVEVKFILASVFMEAFKFYWALNVGKKIRFP
                     ESGLIRGFRRSKGEGKYSFKDLLEEACTDVGCKKTETGFIDNRNALFHSGAFKYEQMN
                     SSESKKQIVPELFTLYRQIDEILLRLIGYAGPIYEYGKPDEPVIFPFGGAGGAEGEEP
                     KGSEGVRAQEL"
     gene            complement(2992..4197)
                     /locus_tag="PKH10_02710"
     CDS             complement(2992..4197)
                     /locus_tag="PKH10_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT27067.1"
                     /translation="MTRLFFWLAMMSLVFVLACEDKKPSNSVCGNKIIEGSEVCDKNL
                     KDCDKIDPNYISGTATCEADCNGYDVSACVESVCGNKIKEGVEVCEQDEQIDCGDIPD
                     QNYSAGTMVPCKDDCTGYVTTNCVEPICGDKVKEGVEVCEQDDQIDCGDMPDKNYMPG
                     TMASCKDDCTGYDESGCVISDADGLVNDVTIPDTTDIDMQDEDSFVSDAADDETNDMG
                     IDEDVIIPDEDTDFTIGTMSCNAEVCIDSTTNLAWSRARAPQSNMTQDAAVSYCNNLS
                     FDGADNWRLPNINELRSLIRGCLLTVTGGACAVIDPSCLSSSLACFSMETCAACVEAG
                     GSGEFGFYWDAGVWLGDGVMGLGIYFWSSSLVSGNQNEAWIVDFDYGTVRYQGKEKSA
                     WVRCVRGPL"
     gene            complement(4306..4791)
                     /locus_tag="PKH10_02715"
     CDS             complement(4306..4791)
                     /locus_tag="PKH10_02715"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNT27068.1"
                     /translation="MRSMVLAAVLFMAIPAFALNVACVDMKKVWENSKEIKAEKANLE
                     KVLKKTQEDLKKKEADLKALQEKALKGAQTMTDEAKQAMQEEFQKKYLEFQELYQSSQ
                     KMLQEKDYTAQSSFIEKVRKIATEIAKKKGYDLVLAKDNVLYMQDKYDITQDVIGVIN
                     K"
     gene            4960..7101
                     /locus_tag="PKH10_02720"
     CDS             4960..7101
                     /locus_tag="PKH10_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNT27069.1"
                     /translation="MHRILCLALFAGLLACSRGPIVAHQARPLPPYPETKRESLVETL
                     HGVEVADPYRWLESTEDPAAQAWTEAQNAFTMKYIEALPQYAPLKKRLAEIWDFDKMQ
                     PPVPRKGKLFFRKTTGLQNQPVLYLRENGVDAALIDPNTLSADGTTAMDWWYASPTGK
                     YIAYGLSEKGSEQSVLHLYDVATKKDVDTPIAGCRYAAVSFLPDETGFFYSRKLDGAT
                     PGEIDTDQSVRFHRIGDDPEKDEVIAKATLKEGIIVSGLDETGRWLLISEYQGSSGKA
                     RLVLYDAKGKTARPLVDNFDNIWEGDIFRGKIYLRTDRDGALNWKIVRVDAETLAWEE
                     FIPAHEKDILDAFLVTGDHIAVHYLHDVASQVVLFDLTGKRVATPALPTIGSVHGMAG
                     SPDEKELFLAFSSYAYPPAILRWTPAEGLTEFFRAAVPVDPSLLETTQVFYPAADGTK
                     IPMFLVHKKGIEKNGMNPTALVGYGGFNVSYPLHFSPANWGWLERGGIYAVANIRGGG
                     EYGEAWHRAGMLGAKETCFNDFADAMRWLIIEKYASPATIGIMGGSNGGLLTGALVTR
                     EPLLFSAALVAVPLLDMLRFHKFLIDRYWVSEYGSADEPEQFQWLYRYSPYHKVKQGE
                     RYPAVLLTAGEHDSRVHPLHAKKMAAALQWANTSKDPILLYVDTKAGHGQGKGTEMRI
                     AEAAMEWAFFMDRLGMATATASPEPSPASAP"
     gene            complement(7034..8524)
                     /locus_tag="PKH10_02725"
     CDS             complement(7034..8524)
                     /locus_tag="PKH10_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNT27070.1"
                     /translation="MADRRILLPLLFLLAFPLTGKDAAPDASSDGSVPDALSYTPAGE
                     DPVRTDVAREAEKAFYVKGTMIALRETASASGRKLLILQNGDIVNFTGVRLSDQGREW
                     MKVTRFTPKGGTVEGWVDSRYLSETKIEGKFSFLKTVDLTPFPKTKGYKENPPVKTRG
                     VYITVYSATPKNIRRYFNMAENGQINTFVVDVKNVEGEILFKSAAAEKHFPAANKRVV
                     YKTTPPFIAEARQKGIYLIARMTAFKDNYYALAYPDRGIMVPGTDELLRDRDKLVWVT
                     PHDRHYWEYLIDLSLEAAAMGFHEIQFDYIRFPDLNKEMDTRNALGESRPQAIQNFLK
                     YAYERLSKAKVYVSADIFGLVPSVADDMAIGQHWETISNVVDYICPMMYPSHYATGFA
                     GLKVPDSDPYKTIRQSALDSVRRNKKLSTPAIIRPWIQDFTAFWVKEHIPYGAKEVED
                     QIRALRELGIDEYLIWNPRNRYTPITVPKPVKAPETPSPSPSPAGP"
     gene            complement(8517..>8835)
                     /locus_tag="PKH10_02730"
     CDS             complement(8517..>8835)
                     /locus_tag="PKH10_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27071.1"
                     /translation="PQDPAKQCRCIELTEGLDYLAYRIGVSVAFGGPTTECGHDALVV
                     FSRTGVPEQYQLAVSTRCGYIKYPPTEKFPFGEPRAYFPSIVGGYIDRARSDGVDCEC
                     ADG"
BASE COUNT         1848 a         2597 c         2306 g         2084 t
ORIGIN      
        1 tttctgcacc gcctcgctgc ggcgcatccg ttcgcattcc cagccggtca ccggatagag
       61 ggcgtcccac gcatcgaata gtttccggtt cttgtccgat atgacgccgc gccccggata
      121 ggcgcggtcc atgtaaaggt aggtgcgggc gatgagtccc cggatcgcct cgcggggctc
      181 tatcttgcgg tcctctatct ccacatcgca ggcgccgtac tcccgtttct cccccggtat
      241 ctcggccatg gagaaattgg aacggtcgcc gttcacctcc cccaccgccg ggtagaggtt
      301 gtacatgtcg gcctccatgc gccggaactc gttgctcgtc agccgcgcgc accgccgccc
      361 cttgaacgaa cgaccttttt tatccgcgca gtcggagtgg ccgtcgcgcc attcggcgaa
      421 actctggccg aaggcgtggg cagggacgac atgttcccac tcgagacgcg cggcgcgctg
      481 cgggtccttc cgcggggtgt agccgcaggc gtcgaggtct atctgcttct tctcgtcgaa
      541 cggacaaccg cagtagatgg tctcgcggtg gtcgtaatag accttttcca tgagcagttt
      601 cttcgccgcc tcgaaatcgg ccaccgcgtg cggcgcgggc gcggagagcg gcaacagcgg
      661 ctcgatgcgg tcccagttga gataggtgat gatcgaaagg aggatgataa agaaaatggt
      721 ccttttcatg gggccattgt cttttattta cgaagggaag tcaactcgtc tgtttatata
      781 ggtgcgccca ccagaacccg cttcaggacg cgaggccgta ctgtttgcga atgttttcga
      841 ggtcagattt catcagcgcg gggtttttca aataatcggc gaaaagcgcc gttcttatgg
      901 cacgcatcat ctcgacgcat ttcggcgttg tcggtttcgg cgtgatcttc cgtttcttct
      961 caatcctatt cttcatctat cacctccctt ggtgaacgga tatctatcat cgaatagccg
     1021 tgcttgaggt taacggcatt gtatcgctgt atgcgtcgca cattgaccat gtgtttaaag
     1081 ttccaactta caaggacatc ggcccggcac accgttgcaa tggcgatatg gtatgcgtcg
     1141 ccttcgaact tgggactcac ggctttctct tttatgtatt cttgagcaag cgcttcgcat
     1201 tccttggtca gcgcaacgat ctcgcggtgt ttttccggaa tggcttcgaa taccgaccgg
     1261 acctcacgcg gggcaagact cagttccttg atggttatgt cggagatgac ggcggtcttc
     1321 tttcccttgc ttatctcgga aagcaaggcg tttgaccata cgccaaattc tttttcagaa
     1381 catccgccga ataccgaggt gtcgatgtag acacgaaaac catgtttcat cgggacactc
     1441 ctccctaacg aacaaaagca tgatagccta tcggtgggca agtgtcaagc gaagggcgtt
     1501 cacgattcac ttctcacgat ttctgccagt tctatagttc ttgcgccctg accccttctg
     1561 accccttcgg ctcctcccct tcggctcctc cggctccccc gaaaggaaag attactggct
     1621 catccggctt tccgtattca tatattgggc ctgcatagcc tattaagcga agaagaattt
     1681 catcaatttg acggtaaaga gtaaacaact ccggcactat ttgttttttc gactccgacg
     1741 aattcatctg ctcatattta aatgcaccgg agtgaaaaag tgcatttctg ttgtcaataa
     1801 aaccggtttc tgttttctta cagcctacgt cggtacaggc ttcttcaaga agatccttga
     1861 aactatattt cccttctccc ttggatcgcc tgaaccctct aatcaggcca ctctctggaa
     1921 agcgaatttt ttttccgaca ttaagagccc aatagaactt gaatgcctcc atgaagacgc
     1981 tggcaaggat aaactttacc tcaacgattt catcggcaat agagaggcag tagaactgga
     2041 atagtctagg aagattatat tcttcttggt tcttggtata ataaggccag gcgttttcta
     2101 tgaatttcgc aagtttttcc gcatctcttg aaatcatcgg gtgggcagat cgcaacccct
     2161 tgttaggaaa tatggatatc tttgaaagag tagttccgtt atatgttttc ctgattggag
     2221 aaaagaaaac gttcctttgc gccagcgaga gaagcatcag aatgtcagaa gagataccaa
     2281 gctgttcgtc tgctctcaac gtagcgatgc cccttaagtg atcaaatacc aacaaacatt
     2341 tttttccccc acaagtgaac cttacttctc tttttcccgt tgtgtttaaa ttaacgatga
     2401 catgatcttc gaattctggc agcaaagtat cgtcagcagt taaaatagcc ttttcaaaac
     2461 taaatctggc ctcgtatttt acaggattcc cgacctcatg tcgctcgaac ttatcaacac
     2521 accacacgcc tcttatttca aaagccattc cttcttttgt ttttccagtc atgcgagcgt
     2581 tttcgacaag agaacaatta aagcaatctc ttgtctgaaa ctctgcgtcc ccattattgt
     2641 aacaaacgat tttcccttcg catgccgatt tgttgccatc cttatcgatg acaaccactt
     2701 cagaacatac aaattcttcc agaatatcag acagatcatt catcttttac tccaccagcc
     2761 gcttcgtcta tcccatgaga ataactgacc ccaaagtttc gcttctcgca caacaatctc
     2821 cttgcgtcgc ttatgttcca ttccttcttt agcctgcact tgaaacatga tagcacctct
     2881 cgtggaaatt aactgagaag agtctgctgc aaagatttcg aattgtcaat atttggtatg
     2941 atctcgggac cttgctaaaa tcttgtcaag aacaattatg agaaagaaag tctacagcgg
     3001 ccccctaaca catctcaccc atgcggattt ctccttccct tgatacctta ccgttccgta
     3061 atcaaaatca acaatccatg cctcgttttg attgcctgat accagagaag aagaccaaaa
     3121 atagattcct aaacccataa caccatcacc aagccaaaca cctgcatccc aataaaaccc
     3181 gaactctcct gatcccccag cttcaacaca ggcagcgcat gtttccatag agaagcaggc
     3241 taaagaggaa gacaagcaac tggggtcaat taccgcacat gcacccccag taacagtcag
     3301 cagacagcct cttattagac tacgcaattc atttatgttg ggcaatcgcc aattatccgc
     3361 cccatcaaag gacagattgt tgcaataaga tacagcagca tcttgcgtca tgtttgactg
     3421 cggggcccgc gctcgtgacc atgcaagatt tgttgtgctg tctatgcaga cctcggcatt
     3481 gcaagacatg gttcctattg taaagtcagt gtcttcgtca ggtattataa catcttcatc
     3541 tatgcccata tcgttagttt catcatctgc cgcatccgaa acaaatgaat cttcatcttg
     3601 catgtcaatg tcagttgtat caggaatcgt tacatcatta accaaaccat cggcgtcaga
     3661 tataacacat ccgctttcat cgtatcctgt gcagtcgtct ttgcaggaag ccatcgtccc
     3721 gggcatataa tttttatcag gcatatctcc gcagtcgatt tgatcatcct gttcacaaac
     3781 ttccaccccc tccttaacct tgtctccgca aatcggttca acacaatttg ttgtcacata
     3841 acccgtacag tcatccttgc agggaaccat cgttccggca ctataatttt gatccggtat
     3901 atctccgcag tcgatctgtt cgtcttgttc acagacctcc accccttcct tgatcttatt
     3961 tccacacacg gactctacgc aggcagagac atcgtacccg ttgcaatccg cctcgcaggt
     4021 cgccgtgcca cttatgtagt tgggatcgat cttgtcgcag tcctttagat tcttgtcgca
     4081 gacctcagat ccttcgatga tcttgtttcc acacacggag ttggaaggtt ttttgtcctc
     4141 gcacgcaaga acaaacacca aagacatcat cgcaagccaa aaaaacaacc gcgtcatgtc
     4201 ggcctcctat ttttcccaga aagaacgacc gattgtaacg aattgaagag gtaaatgcaa
     4261 gaaagagggc gcgatggatc acgcccctac aaaaggggaa ggatcctatt tgttgatgac
     4321 gccgatgaca tcctgcgtga tgtcgtactt gtcctgcatg tagaggacat tgtccttggc
     4381 gagcacgagg tcatagccct ttttcttggc tatctcggtc gctatcttgc ggaccttttc
     4441 gataaaagag gactgcgcgg tgtagtcttt ttcctgcagc atcttctggc tcgactggta
     4501 gagttcctgg aattcgagat atttcttctg gaactcctcc tgcatcgcct gcttcgcctc
     4561 gtcggtcatc gtctgggcgc ccttcaacgc cttttcctgc aacgccttga ggtcggcctc
     4621 tttcttcttg aggtcctcct gcgtcttttt gagcaccttc tccagattgg ccttttctgc
     4681 ctttatctcc ttggagttct cccagacctt cttcatgtcg acacaggcga cattgagcgc
     4741 gaaggcgggg atcgccatga acaggacggc ggccagtacc atagatctca taggttcctc
     4801 ctcacgggta agatcgtcat ctcctccgtc gtgcgacggt cgggccatca tacccgctct
     4861 ttcccgaatt gcaacttgag catttttttt gccttgccgc ccgcttcgca ctagagtagg
     4921 agcggtcttc cgttcttatc tgatgaaagg aggcgcatca tgcaccggat cctttgcctt
     4981 gccctgttcg cggggcttct cgcctgttcg cgcggcccga tcgtcgccca tcaggcgcgg
     5041 ccgctccccc cctaccccga gacgaagcgc gagtcgctcg tcgagacgct ccacggcgtc
     5101 gaggtcgccg acccctaccg gtggctcgag agcaccgagg acccggcggc gcaggcgtgg
     5161 accgaggcgc agaacgcctt cacgatgaag tacatcgagg cgctcccgca gtacgcgccg
     5221 ctgaaaaaac ggctcgccga gatatgggat ttcgacaaga tgcagccgcc ggtgccgcgt
     5281 aaaggcaaac tctttttccg caagacgacc ggcctgcaga accagccggt cctctacctg
     5341 cgcgagaacg gcgtcgacgc cgcgctcatc gacccgaaca ccctgtcggc cgacgggacg
     5401 accgcgatgg actggtggta cgcctcgccg acagggaagt atatcgccta cggcctttcg
     5461 gagaaggggt ccgaacagtc ggtgctccac ctttacgatg tcgcgacaaa gaaggatgtc
     5521 gacaccccca tcgccgggtg ccgctacgcc gcggtgagtt ttctgcccga cgagaccgga
     5581 tttttctact cgcgcaaact cgacggcgcc actcccggtg agatagacac cgatcagtcg
     5641 gtgcgattcc accgcatcgg cgacgatccc gagaaggacg aggtcatcgc gaaggcgacg
     5701 ctcaaggagg gcatcatcgt ctcgggactc gacgagacgg ggcgctggct cctcatctcc
     5761 gaatatcagg ggtcctccgg caaggcgcgg ctcgtcctgt acgacgcgaa gggaaagacc
     5821 gcccgcccgc tcgtcgacaa tttcgacaac atatgggaag gcgacatctt ccgcggcaag
     5881 atatacctgc ggaccgaccg cgacggcgcg ctgaactgga agatagtccg ggtcgacgcc
     5941 gagacgctcg cgtgggagga attcatcccg gcgcacgaga aggacatact cgacgcgttc
     6001 ctcgtgaccg gcgaccacat cgcggtccac tatctgcacg atgtcgcctc gcaggtggtg
     6061 ctgttcgacc tgaccggcaa aagggtagcg acccccgcgc ttccgacgat cgggtcggtc
     6121 cacggcatgg ccggatcgcc cgacgagaag gaactgttcc tcgccttctc cagttacgcc
     6181 tatccgccgg cgatactgcg ctggacgccc gcggaggggc tcacggagtt cttccgcgcc
     6241 gcggtcccgg tcgatccctc ccttctcgaa acgacgcagg tcttctaccc cgccgccgac
     6301 ggcacgaaga taccgatgtt cctcgtgcat aagaaaggca ttgagaagaa cgggatgaac
     6361 cccacggcgc tcgtcggtta cggcggcttc aacgtcagtt atccgcttca cttctcgccc
     6421 gcgaactggg gctggctgga gcgcggcggc atctacgcgg tcgcgaacat ccgcggcggc
     6481 ggcgaatacg gcgaggcatg gcaccgcgcc gggatgctgg gcgccaagga gacctgcttc
     6541 aacgacttcg ccgacgcgat gcgctggctc atcatcgaga agtacgccag ccccgcgacg
     6601 atcggcatca tgggcggcag caacggcggg ctcctgaccg gcgcgctcgt cacccgtgaa
     6661 ccgctcctgt tctcggcggc gctcgtcgcg gtgccgctcc tcgacatgct gcggttccac
     6721 aaattcctca tcgaccgcta ctgggtcagc gaatacggct cggccgacga gcccgagcag
     6781 ttccagtggc tgtaccgcta ctcgccctac cacaaggtga aacaggggga acgctatccc
     6841 gccgtcctgc tgaccgccgg cgaacacgat tcgcgcgttc acccgctcca cgccaaaaaa
     6901 atggcggcgg ctcttcagtg ggccaatacc tcgaaggacc cgatactgct ctatgtggac
     6961 accaaagcgg gacacggtca ggggaaaggg accgagatgc gcatcgccga ggcggcgatg
     7021 gaatgggcct tctttatgga ccggctgggg atggcgacgg cgacggcgtc tccggagcct
     7081 tcaccggctt cggcaccgtg atcggggtgt agcggttgcg cgggttccag atgaggtatt
     7141 cgtcgatgcc gagttcgcgg agcgcccgta tctggtcctc gacctctttc gcgccgtaag
     7201 gaatgtgctc cttcacccag aaggcggtga agtcctgtat ccatggccgg atgatcgccg
     7261 gggtcgatag ttttttgttg cgccgcaccg aatccagcgc cgactgccgt atcgtcttgt
     7321 acggatcgct gtcgggtacc ttgagccccg cgaacccggt cgcgtagtgg ctcgggtaca
     7381 tcatcgggca gatatagtcc acgacattcg atatcgtctc ccagtgctgg ccgatcgcca
     7441 tatcgtcggc gacgctcggc acgagcccga agatgtcggc cgacacatag accttcgcct
     7501 tcgagagccg ctcatacgcg tatttgagga agttctgtat cgcctgcggc ctgctttctc
     7561 cgagcgcgtt gcgggtgtcc atctccttgt tgagatcggg gaagcgtatg tagtcgaact
     7621 gtatctcgtg gaaccccatc gcggccgcct cgagcgacag gtcgatgaga tactcccagt
     7681 agtgacggtc gtgcggagtg acccacacca gtttgtcacg gtcgcgcagg agttcgtcgg
     7741 tccccggcac catgatgccg cggtcggggt aggcgagcgc gtagtagttg tccttgaagg
     7801 cggtcatccg ggcgatgagg tagatgccct tctgccgcgc ctcggcgatg aagggtgggg
     7861 tcgttttata gacgacccgc ttgttggccg ccgggaaatg cttttcggcc gccgcacttt
     7921 tgaacagtat ctcgccctcg acattcttca catcgacgac gaaggtgttt atctgcccgt
     7981 tctcggccat gttgaagtag cggcggatgt tcttcggggt cgccgaatag acggtgatgt
     8041 agaccccgcg cgttttcacc ggcggattct ccttgtaacc ttttgtcttg gggaacggcg
     8101 tcaggtcgac cgtcttgagg aacgagaact tcccctctat cttggtctcc gagagatatc
     8161 gtgaatcgac ccatccctcg accgtgccac ccttcggggt gaagcgggtc accttcatcc
     8221 attcgcgtcc ctgatcgctg aggcggacac cggtgaagtt cacgatgtcc ccgttctgca
     8281 ggatgagcag tttgcgcccg ctggccgaag cggtctcgcg caacgctatc attgttcctt
     8341 tcacatagaa cgccttctcg gcctcccgcg cgacatcggt gcgcaccgga tcctctccgg
     8401 cgggggtgta actcagcgcg tcgggcaccg acccgtcgga tgacgcgtcg ggcgccgcat
     8461 cctttccggt gagcgggaac gcgagcagga aaagtagcgg gagcaggatg cgacggtcag
     8521 ccatcggcgc actcgcagtc gacgccgtcc gaccgcgcac ggtcgatgta gccgccgacg
     8581 atggaaggga aataggcccg cggttcgccg aacgggaact tctcggtcgg cgggtatttg
     8641 atgtagccgc accgggtgct cacggcgagt tggtactgct cgggaacgcc ggtgcgggaa
     8701 aagacgacga gcgcgtcgtg cccgcactcg gtcgtcgggc cgccgaaggc taccgagacg
     8761 ccgatacggt acgcgaggta atcgagtccc tcggtcagtt cgatacatcg gcactgtttc
     8821 gcggggtcct gcggg
//
LOCUS       DAOQUE010000053         8793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8868_length_8793_cov_4.320783, whole genome shotgun sequence.
ACCESSION   DAOQUE010000053 DAOQUE010000000
VERSION     DAOQUE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8868_length_8793_cov_4.320783"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PKH10_02735"
     CDS             <1..585
                     /locus_tag="PKH10_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT27072.1"
                     /translation="VSRGDFLAITGQSGSGKTTLLNIMGLLDRSTTGNLLVNGTDTAG
                     MDDEAITALRGRTLGFVFQFHHLLPALTILENVMMPMMADKGREMPGMRESATELLKR
                     VGLGERLKYKPDEVSGGQRQRAAIARALAMRPAVILADEPTGNLDSVSSAEVFTLLRE
                     INKADGTAFCIVTHDAAIAKTCDRIVEIVDGRLV"
     gene            659..2287
                     /locus_tag="PKH10_02740"
     CDS             659..2287
                     /locus_tag="PKH10_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNT27073.1"
                     /translation="MRWKEYRLLFVHLILCFAFALYGGGESDGRPVRVGVYENKPKIF
                     TDEQRNASGIFISILEEIARKEGWRVTYVWCEWFDCLELLSAGQIDLLPDVALSPDRS
                     RRFDFHKEEVVNSWSAVYAPSRREIERMADLHGHRLSVLKGSIQQTTLEEMAKGFGFA
                     ITFVEANSFEEAYRLVEEGQADAVVANHLSGDHFHRQYGLEKTPVVFQPVSLFFATTP
                     DTNGDLLAAIDKDLRAMKSEPESTYYKALWRWMDRPPAVIVPPHLVWGIGVVGGLLVL
                     AILFILLLRWQLRVRTRHLAEANERLRQAQKMDAVGRLAGVMAHDNNNMLTIILGCAE
                     MGISKSRPDDPARGYFQEIANAAKRSADITRQLLAFARKQETVPRSLDLNQSVDGMLK
                     MLRPLIRETIALDRELAADLWPVWMDPGQVEQVVANLCVNARDAIADAGRIVIETRNE
                     SFDKAHCGAHPDHIPGDFVRLTISDNGSGMTTEVLDHLFEPFFTTKGEEKGSGLGLST
                     VYGIVTQNGGFVTVESAPGRGTTFKVHLPRHRSG"
     gene            complement(2294..2914)
                     /locus_tag="PKH10_02745"
                     /pseudo
     CDS             complement(2294..2914)
                     /locus_tag="PKH10_02745"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
     gene            complement(2911..4386)
                     /gene="crtI"
                     /locus_tag="PKH10_02750"
     CDS             complement(2911..4386)
                     /gene="crtI"
                     /locus_tag="PKH10_02750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNT27074.1"
                     /translation="MKRIAVIGAGFGGLAASAILAHRGYAVEVFEQQSGPGGKAGDLR
                     LDGWRFDTGPSLFTMPAVFEKCFALAGGDLNAALTHEPMELITRYFWDDGSTLDAWQD
                     TDRFATEVEKKLGDGAEAVKRYLKRAKKIYDTTADIFLYGDPWDPLNIVRLSGWRALA
                     GLPFIAPFTTMDRFHRRYFKDERTIQLFDRYATYTGSDPYRAPATLAIIPHVEYAIGA
                     RNLRGGVTALVRAMHTLAEKNGAIFHFNAPVDRILKKNERAAGIVTGGADIPFDAVVS
                     NADVTHTYLSLLQSESEAADRYQRHEPSVSGVVFYWAMRETFPELTVNNIFFSADYRR
                     EFEEIFDRHRCGSDPTVYVNITSKFDAADAPAGGENWFVLVNAPYDDHQDWDIEVTRL
                     RDTVIAKVSRLIGRDIEPFIAHEQVRTPKILRELTNTNHGSIYDISSNDPMAAFRRQP
                     ARCADHPGVFFCGGSAHPGGGMPLATLSGMMAADAVTRRMK"
     gene            complement(4383..5057)
                     /locus_tag="PKH10_02755"
     CDS             complement(4383..5057)
                     /locus_tag="PKH10_02755"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HNT27075.1"
                     /translation="MSAYLAVNLCVIALPLLFSFDKRVAFWRKWPAALTAIVTVGAIY
                     LAWDSHATGAGHWAFNPAYVGDLRLAGLPIEEWLFFVTVPYACLFTYECIRVYLAPGR
                     VPFMRPVLAAAAVAAIILAFLSVGKGYAFLAFSGLFLVLVFYLVFLPRLTEERSFVVA
                     MGIFFLLFGIVNSVLTGIPIVTYDPAAITGLRIGTIPIEDFVYNFIMLAGHLAIYRTI
                     LDRRSA"
     gene            complement(5057..6112)
                     /locus_tag="PKH10_02760"
     CDS             complement(5057..6112)
                     /locus_tag="PKH10_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNT27076.1"
                     /translation="MKKYLAASKERTTAYLGRFLADKETELAPIGPRAAAVARDIHEF
                     CTRGKMIRAAMTLLGWEAAGGRPSDTVIPVAAAMEMIHAGLLIHDDVMDRDDFRRGKP
                     AFHRRYADALAHETKEPRHTGEALAICAGDVAFFLAAESVMTAAIPDPHGAAMRRTFF
                     RECVLVGLGQMDDVYLGARLAPVTVADILPVYRYKTARYTFSMPLILGAIAAGGDAKV
                     RDGLDAVGEKIGTLFQIKDDELGLFGEEADIGKPTGSDIREGKKTILFSLLTDMAHGT
                     DKERLSRTLGNPDIGSDDIAFVRTLAEKSGALGKVRAQAATIDGEARGLIAALTVGDE
                     VKRLLNELIDYNRDRGF"
     gene            complement(6109..6939)
                     /locus_tag="PKH10_02765"
     CDS             complement(6109..6939)
                     /locus_tag="PKH10_02765"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNT27077.1"
                     /translation="MAHNATKGKIFLKGSKTYFTSSIFFPPQVRADVFTLYAFLRVAD
                     DLVDCIPQRADEFYEMRRKWRIACTGTPAHDPVIDDFVALAERIGFEPEWTEAFFHSM
                     ELDLAKKEYNTLPETLEYVYGSAEVIGLYMMCIMGLPPESEHPARMLGRAMQYINFIR
                     DINEDIGLGRRYLPLRDSLLTSLLPDETHKKPDAFRRFITEEIERYLGWQKEAEAGFC
                     YLPRRYRIPVATASDMYAWTAATIRRDPFVVYERKVKPSRLRIVANIIRRFAAIRECP
                     "
     gene            7086..7220
                     /locus_tag="PKH10_02770"
     CDS             7086..7220
                     /locus_tag="PKH10_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27078.1"
                     /translation="MSRLLVVIFAMLLAGCGDNFDRDELMNGPRQPDGALDTDILITE
                     "
     gene            7224..7508
                     /locus_tag="PKH10_02775"
     CDS             7224..7508
                     /locus_tag="PKH10_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT27079.1"
                     /translation="MPRTSLILIFCLLAVTSLFLSAASKEEKHQVDPKTCIGCALCVQ
                     SCPSKAIVMKDGKAIIDPERCINCTLCVQKCPTKAIRGPTVKPAAEKKTK"
     gene            7562..7753
                     /locus_tag="PKH10_02780"
     CDS             7562..7753
                     /locus_tag="PKH10_02780"
                     /inference="COORDINATES: protein motif:HMM:NF025121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT27080.1"
                     /translation="MKKVQYIQQARCTGCRECLPVCPTKAIVLRTGKAVIDPEKCIDC
                     GLCVKACSFGAPVEREVKK"
     gene            7750..8013
                     /locus_tag="PKH10_02785"
     CDS             7750..8013
                     /locus_tag="PKH10_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT27081.1"
                     /translation="MRRLIRVLLLGGMLVSCGMDPSLDSPITINHDRCVGSTCRVCER
                     ACPYDAIHFYGPEDKPVVDPDKCTQCGICVRECPYQAITGRPQ"
     gene            8010..>8793
                     /locus_tag="PKH10_02790"
     CDS             8010..>8793
                     /locus_tag="PKH10_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27082.1"
                     /translation="MRRLAALMMLSALSVALYGGGEVTAGGYVPFMESKPTDPAAPGY
                     YFVTGHALYEPVDGFFIGGDLFFLHGFPEHDMTNLVETSYLLGRGRLLVQYDWPKVKI
                     GTVTHASISDDSGDYAKSITQGRATLGGYRRDHLHEELYLTAEPLEGLRFTASTGVVS
                     RSFAVDPEQGSGVIRDTDLFTYGDLSYEIFPALEPFAGVLYSDDLNASDAFDLLRFRG
                     GLRGGELFFDKRLHLSYALYYKREEAGSLNDKDRLALYIKGRW"
BASE COUNT         1702 a         2545 c         2856 g         1690 t
ORIGIN      
        1 gtgtcgcgcg gcgatttcct cgcgatcacc gggcagtcgg ggtcgggcaa gacgacgctt
       61 ctgaacatca tggggctcct cgaccggtcg acgaccggga acctgctggt caacggcacc
      121 gataccgccg gtatggacga tgaggcgata acggccctgc gcggccggac gctcgggttc
      181 gtcttccagt tccaccatct cctcccggcg ctcaccatcc ttgagaatgt gatgatgcca
      241 atgatggccg acaaggggcg cgagatgccc ggtatgcgcg agtcggcgac cgaactgctc
      301 aagcgggtgg ggctgggcga acggctgaaa tacaaacccg acgaggtatc gggcggccag
      361 cgtcagcggg cggcgatagc ccgggcgctc gccatgcggc cggcggtcat cctcgccgac
      421 gagccgaccg gcaatctcga cagcgtcagt tcagccgagg tcttcaccct cctgcgcgag
      481 atcaacaagg ccgacggcac cgccttctgc atagtcaccc acgatgccgc catcgcgaag
      541 acctgcgacc gcatcgtcga gatcgtggac gggcggttgg tttaggaaca ttccggaaac
      601 cttgcgattt tttttctgtc ggatagaatc tttagcggat caccgggtgg ggtgcctcat
      661 gcgttggaaa gaatatcgtc ttctctttgt tcacttgatc ctttgctttg ccttcgcact
      721 gtacgggggc ggggaatccg acgggcggcc ggtccgggtc ggggtctatg agaacaagcc
      781 gaagatattc accgatgaac agaggaatgc gtcgggtata ttcatctcga tactggagga
      841 gatcgcacgg aaagaggggt ggcgggtgac ctatgtgtgg tgcgagtggt tcgactgtct
      901 cgaactgttg agcgccggtc agatagacct cctgcccgat gtcgccctct cgcccgatcg
      961 atcgcgccga ttcgatttcc ataaggagga ggtggtaaac agttggtcgg ccgtttatgc
     1021 gccgagcaga cgggagatag agcggatggc ggacctccac ggtcatcgcc tttctgtgct
     1081 caaagggtcg atccagcaga cgacgctgga ggagatggcg aaggggttcg ggttcgctat
     1141 caccttcgtc gaggccaatt cgttcgagga ggcctaccgg ctcgtggagg agggacaggc
     1201 cgatgcggtc gtcgcgaacc atctttcggg cgatcatttc caccggcagt acggacttga
     1261 aaagaccccc gtcgtttttc agccggtctc gctcttcttt gcgaccaccc ccgataccaa
     1321 cggtgacctt cttgcagcca tagacaaaga tctgcgagcc atgaagtcgg aacccgaatc
     1381 gacctactac aaagcgctgt ggcgctggat ggatcgtccg cccgcggtga tcgttccgcc
     1441 gcatcttgtg tggggcatag gagtggtcgg gggcctgctt gtactggcga tacttttcat
     1501 acttctcctg cgctggcagt tacgggtccg gacacgccat ctggccgagg cgaacgagcg
     1561 gttgcggcag gcgcagaaaa tggacgcggt cggccgtctt gccggggtca tggcccacga
     1621 caacaataat atgctgacca tcatactcgg ctgcgccgag atggggatat cgaaaagcag
     1681 accggacgat ccggcccgcg gttactttca ggagatcgcg aacgccgcca agcgttcagc
     1741 cgatatcacc cgccaactgc tcgcctttgc ccgcaaacag gagacggtgc cccgatcgct
     1801 cgacctgaac cagagcgtgg atgggatgtt gaagatgctc cgaccgctta tccgcgaaac
     1861 catcgcgctc gatcgggaac tcgccgccga cctctggccg gtgtggatgg atccggggca
     1921 ggtcgaacag gtcgtggcga acctctgcgt caatgcgcgg gacgccatcg ccgatgcggg
     1981 gaggatcgtc atcgagaccc gcaacgagag ttttgacaag gcccactgtg gcgcccatcc
     2041 cgaccatatt cccggtgact tcgttcgcct gaccatcagc gataacggaa gcggcatgac
     2101 cacagaggtg ctcgatcatc ttttcgagcc gttcttcacc accaaggggg aggagaaggg
     2161 gagcggcctt ggactttcga ccgtttacgg catcgtcacg cagaacggcg gtttcgtcac
     2221 ggtggaaagt gcgccgggca ggggaacgac cttcaaggtc catctgcccc gccaccggtc
     2281 agggtaactt ccttcacgcc cagcgcaata ccgcgaagaa caccaactga atgatgaaat
     2341 accacgcggg gagaatggtc tttgtcttca tcttgaagag gcggtagata tagcctgcga
     2401 tggcggcggt gatgaaccac gcggcgtagt tctgaagcgg gatctccgcc gtatgccagt
     2461 tccagaagtc gagccgtatc gcgaccggct acatcgccca gtccaacccg gtggcgagcg
     2521 ccgcgacgac gagcgccgag gaaagcggtc gcaccgtcag tcgttccgag aggcggagcg
     2581 cccccagcac gacgatcacc cagttcgccc cgatgattag cggcacggca agtactttgg
     2641 gaccgagcgt cgcgccgtag tggtaggggc cgaagacaag gccggtcgca acccccagcg
     2701 cctcgagcgc gaaggtggcg gcgtaggtga gcgggaacca gaggagcatc tttttatcgt
     2761 cgcgcaccga cgggacgagc atgatgagcg tgaaagcaag gagcacgagc ggcgttatcg
     2821 acaggaaaaa tggtaaggtc tcggcgaaaa gatgccccgc gatgccaacg aggtagaaaa
     2881 tactgagcgc gatgatgaac cagtgcttct tcatttcatc ctccgcgtca cggcgtcggc
     2941 tgccatcatc cccgacaggg tcgcgagcgg catcccgccg cccgggtggg ccgaaccgcc
     3001 gcagaagaac actcccgggt gatcggcgca tctcgccggc tggcggcgga aggcggccat
     3061 cgggtcattg gaggagatgt cgtagatgga accgtgattg gtgttggtga gttcgcgtag
     3121 tattttcggg gtgcggacct gttcgtgggc gatgaagggc tcgatgtcgc gcccgatgag
     3181 ccgcgagacc ttcgcgatga cggtgtcgcg caggcgggtc acctcgatat cccaatcctg
     3241 atggtcgtcg tagggggcgt tcacgaggac gaaccagttc tcgccgcccg ccggtgcgtc
     3301 ggccgcgtcg aacttcgagg tgatgttgac atagacggtc gggtccgatc cgcatcgatg
     3361 gcggtcgaat atctcctcga actcgcggcg atagtcggcg gagaagaaga tgttgttgac
     3421 ggtcagttcg gggaaggtct cgcgcatcgc ccagtagaag acgacgcccg ataccgacgg
     3481 ttcgtgccgc tggtagcgat cggccgcctc cgactcgctc tggaggagcg agaggtatgt
     3541 gtgggtgaca tcggcgttgg agacgaccgc gtcaaagggg atgtccgcgc cgcccgtgac
     3601 gatgcctgcg gcgcgttcgt tcttcttgag tatccggtcg accggcgcat tgaagtggaa
     3661 tatcgcgccg ttcttttcgg cgagcgtatg catcgcccgg acgagcgccg tcaccccgcc
     3721 gcgcaggtta cgggcgccga tggcgtattc gacatgaggg atgatggcga gcgtcgctgg
     3781 agcgcggtag gggtcggacc cggtgtaggt ggcgtagcgg tcgaagagtt gtatcgtccg
     3841 ttcatctttg aaataacggc ggtggaagcg gtccatcgtc gtgaaggggg cgatgaaggg
     3901 gagtcccgcg agcgcccgcc aacccgagag acgaacgatg ttcagggggt cccacgggtc
     3961 cccgtagaga aagatgtcgg cggtggtgtc gtatatcttt ttcgcccgct tgagatagcg
     4021 ttttaccgct tcggcgccgt cccccagttt cttttcgacc tcggtggcga aacggtcggt
     4081 gtcctgccat gcgtcgagcg tcgagccatc atcccagaag tagcgggtga tgagttccat
     4141 cggttcgtgg gtgagcgccg cgttcaggtc gccgcctgcc agcgcgaagc acttctcgaa
     4201 gaccgccggc atcgtgaaga gcgacggtcc ggtgtcgaag cgccagccgt cgaggcgcag
     4261 gtcgcccgcc ttgccgccgg gaccggactg ctgctcgaag acctcgaccg cgtagccgcg
     4321 gtgggccagt atcgcagagg ccgcaagccc gccgaacccc gcgccgatga ccgcgatccg
     4381 cttcatgccg atctccgatc gaggatggtg cgatatatcg cgagatgtcc cgcgagcatg
     4441 atgaagttgt agacgaagtc ctctatcggg atggtgccga tgcgcaggcc ggtgatcgcc
     4501 gccgggtcat aggtgacgat gggtatgccg gtcagcaccg aattgacgat gccgaacagg
     4561 aggaagaata tccccatcgc gacgacgaac gatcgttcct cggtgagccg cggcaggaag
     4621 accagataga agaccagcac gaggaagagc cccgagaagg caaggaaggc atatcctttc
     4681 ccgacgctta gaaaggcgag gatgatggcg gcgaccgccg ccgccgcgag gacggggcgc
     4741 atgaagggga cacggcccgg tgcgagatag acgcggatac attcgtaggt gaagaggcac
     4801 gcatagggga ccgtgacgaa aaagagccac tcttcgatag ggagtccggc cagtcgcaga
     4861 tcgccgacat aggcggggtt gaatgcccag tgccccgcgc cggtcgcatg gctgtcccac
     4921 gcgaggtaga tggcgccgac cgtgacgatg gcggtgagag ctgcgggcca tttgcgccag
     4981 aaggcgaccc gtttatcgaa cgagaataaa agcgggaggg cgatgacgca gaggttgacg
     5041 gccaaatagg cgctcactag aatccccggt cgcggttgta gtcgatgagt tcgttcagga
     5101 gccgtttcac ctcgtcgccg acagtgagcg cggcgataag accgcgcgcc tcgccgtcga
     5161 tggtggccgc ctgcgcgcgg accttgccca gcgcgccgct cttttcggcc agtgtccgga
     5221 caaaggcgat gtcgtcggag ccgatatcgg ggttcccgag cgtccgcgac agccgctctt
     5281 tgtcggtgcc gtgcgccatg tcggtgagaa gcgagaagag gatggtcttc tttccctcgc
     5341 ggatgtcgga gccggtcggc ttgccgatgt cggcctcttc gccgaagagt cccagttcgt
     5401 cgtcttttat ctgaaagagc gtacctatct tttcgccgac ggcatcgagt ccgtcgcgta
     5461 ccttcgcatc gcctccggcc gcgatggcgc cgaggatgag cggcatggag aacgtatatc
     5521 gcgccgtctt gtagcggtat accggcagga tatcggcgac cgtcaccggc gcgagccgtg
     5581 cgccgaggta gacatcgtcc atctgaccca ggccgaccag cacacattcg cgaaagaagg
     5641 tccggcgcat cgcggcaccg tgcggatcgg gtatcgcggc ggtcatgacc gactcggcgg
     5701 caaggaagaa ggcgacatcg ccggcgcaga tcgcgagcgc ctcgccggtg tggcgcggct
     5761 ccttggtctc gtgggcaagc gcgtcggcgt agcggcggtg gaacgccggt ttgccgcgcc
     5821 ggaagtcatc ccggtccatc acatcgtcgt ggatgagcag tcccgcgtgg atcatctcca
     5881 tcgcggcggc gaccggtatg acggtgtcgc tcggtctgcc gccggccgcc tcccagccca
     5941 gcagtgtcat cgcggcgcgg atcatcttgc cgcgcgtgca gaactcgtgg atgtcacggg
     6001 ccaccgccgc agcgcgcggc ccgatggggg ccagttcggt ctccttgtcg gcgaggaatc
     6061 gcccgagata ggcggtggtc cgttccttgg aagcggcaag gtacttcttc atgggcactc
     6121 cctgatggcc gcgaagcggc gaatgatgtt ggcgactatc cgcagacgcg acggtttgac
     6181 cttgcgttcg tacacgacga aggggtcgcg ccgtatcgtg gcggcggtcc atgcatacat
     6241 gtcggaggcg gtcgccaccg gtatccggta gcggcgcggc agataacaga accctgcttc
     6301 ggcctccttc tgccagccga gatagcgttc tatctcttcg gtgatgaagc ggcgaaaggc
     6361 gtcgggtttc ttgtgcgtct catcgggcag aagcgaggtg agcaacgaat cgcgcaacgg
     6421 caggtaacgg cggccgagcc cgatgtcctc gttgatatcc cggatgaaat tgatgtactg
     6481 catcgcgcgg ccgagcatcc gtgccgggtg ttccgattca ggcggcaggc ccatgataca
     6541 catcatgtag agaccgatca cctcggcaga tccgtagaca tattcgagcg tttcggggag
     6601 ggtgttgtac tctttcttgg cgaggtcgag ttccatcgag tggaagaagg cctcggtcca
     6661 ctccggttca aagccgatcc gttcggcgag cgccacgaaa tcatcgatga ccgggtcgtg
     6721 tgccggggtc ccggtgcagg cgatgcgcca tttcctgcgc atctcataga actcgtcggc
     6781 ccgttggggt atgcagtcga ccagatcgtc ggcgacccgc agaaaggcat acagtgtgaa
     6841 gacatcggcg cgcacctgcg gcgggaagaa tatcgaactg gtgaagtatg tcttgctgcc
     6901 tttcaggaat atctttcctt tcgtggcgtt atgggccatg gcttcgttcc ctgaacagtg
     6961 agcgtgaggc gattatagta aaaagaagat cgattgcaaa atagaatcgc gatccgccgg
     7021 aacttttctc ttgcactttt cgtcagagaa ggtataacgc tccgaaatct gggcacggag
     7081 aacccatgag ccgcctgctg gtcgtgatct tcgcgatgtt gcttgccgga tgcggcgaca
     7141 acttcgatcg agacgaactc atgaacggcc cgcgccagcc cgatggggcc cttgacaccg
     7201 atatcctgat cacggagtga ccgatgccgc gcacgtcgct tattctgatt ttctgcctcc
     7261 tcgcagtgac ctcgttgttt ctatctgctg cctcaaaaga ggagaaacat caggtcgatc
     7321 cgaagacctg catcggctgt gccctttgtg tgcagtcctg tccatcaaaa gcgatagtga
     7381 tgaaggacgg caaagccatc atcgatcccg agaggtgtat caactgcacc ttgtgcgttc
     7441 agaaatgccc gaccaaggcg atccgtggac cgaccgttaa acccgcggct gaaaaaaaaa
     7501 cgaaataatt gacgcttccg gctccgagaa gtcttcgccg agacaagcca tcccccctgc
     7561 ggtgaaaaaa gtccaatata ttcaacaggc gcgctgtacc ggttgccgtg agtgcctccc
     7621 ggtctgtccg accaaggcta tcgtgctgcg gacggggaag gcggtcatag atcctgagaa
     7681 gtgcatcgac tgcggtctgt gcgtgaaggc ctgttctttc ggtgctccgg tcgagcggga
     7741 ggtgaagaaa tgagacgcct catccgcgtg cttctgctcg gcgggatgct tgtctcctgc
     7801 gggatggacc cgtctctcga cagcccgata acgataaacc acgaccgctg tgtcgggtcg
     7861 acctgccgtg tctgcgaacg ggcctgtccc tacgatgcga tacatttcta tggccctgag
     7921 gacaagccgg tcgtggaccc cgacaagtgt acccaatgcg gtatctgcgt ccgcgaatgt
     7981 ccatatcagg cgatcaccgg gaggccgcaa tgaggcgcct cgccgcgctg atgatgctct
     8041 ctgctctgag cgtggcactg tatggcgggg gtgaggtgac ggcaggcgga tatgttcctt
     8101 tcatggagtc gaagccgacc gacccggcgg cgcctggtta ctatttcgtg acagggcacg
     8161 ctctctacga gccggtcgat ggctttttca tcggcggcga tctgttcttc ttgcacgggt
     8221 tccccgagca cgatatgacc aacctcgtcg aaacgagtta tctgctcggt cgagggagac
     8281 tactggtcca gtacgactgg ccgaaggtga agataggcac ggtgacccac gcctcgatat
     8341 ccgatgattc gggcgactac gcgaaaagca tcacgcaggg acgcgcgacg ctcggaggct
     8401 accgtcgcga ccatctgcac gaagaactct acctgacggc cgagccgctg gaaggacttc
     8461 gcttcacggc ctcgacgggc gtggtctccc gttcgttcgc ggtcgatcct gaacagggga
     8521 gcggcgtcat ccgcgatacc gacctgttca cctacggcga cctttcctac gagatattcc
     8581 cggcccttga accgttcgcc ggcgttctct acagcgatga tctcaacgcg agcgacgcct
     8641 tcgacctgct ccggttccgg ggcggtctgc gtggggggga actgttcttc gacaagcggt
     8701 tgcatctctc ctatgccctc tattacaaga gggaagaggc tgggtcgctc aacgataagg
     8761 accgattggc gctctacata aaagggcgtt gga
//
LOCUS       DAOQUE010000054         8751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8918_length_8751_cov_4.812902, whole genome shotgun sequence.
ACCESSION   DAOQUE010000054 DAOQUE010000000
VERSION     DAOQUE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8918_length_8751_cov_4.812902"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1554)
                     /locus_tag="PKH10_02795"
     CDS             complement(118..1554)
                     /locus_tag="PKH10_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT27083.1"
                     /translation="MPRLWSLFFGCSFLFVLSCSTPEETAVDEMAVTDTKVADDTAMD
                     EETKDENDIGNEKDEMVEGEESPDLLDETVDLEGPDGSADTAPDEDTVQPDDVIPVGE
                     LIIEQIYLPGMNMGEAALIVGPDGTTVLIDVANDGHGPQILEAVTRRKGEAAIDWAII
                     THYHNDHIGGFDNLFGAGANQHLEVRKGMILRGFYDIGADMPGVEDFNEFCTLARGEL
                     AAQMVTLCTGVAEMPCGDASSRFPTSDCPGLLLGNLDMSDDDAAGALSYIPLGNGAKL
                     YFTHASGWVAQAGGAISAENNGITIGYGETDQENARSLGGVIKWGDFNYLFAGDTQGR
                     DIKIEGFIASHSAGLTITPGGAELLPSGAADVLHLSHHGLASSTAQEWVDWLFPDNGQ
                     GRNAVVGTTGIYVTSPAQAVLDRVVPRLGTGFVFATANAYTHGTSPQLIIANAAVTVE
                     VSQGGSQYVMVTGVGAGAITSGVYPTVP"
     gene            1709..3241
                     /gene="hutH"
                     /locus_tag="PKH10_02800"
     CDS             1709..3241
                     /gene="hutH"
                     /locus_tag="PKH10_02800"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNT27084.1"
                     /translation="MPLKKLLIDGETLTIRDVYEVACCDRPVGVAPAAAKKVEKCRRF
                     VDKLLTQDKPVYGINTGFGILSEVAIPKDHLDDLQMNLIRSHAIGIGDPFDRATVRAI
                     MLLRANVLAKGFSGVRLSTLEKLVEFINADIVPYIPQKGSVGASGDLAPLSHLALALC
                     GEGYCLGDDMKPVDTAGVLKKKKVTPIALKAKEGLALINGTQVMTAVGALTVERLFGL
                     SKLADISLALTVDAMLGTDRAFDEKVSRVRAHPGQFEVSRNVRKLIAGSTIRESHRCC
                     TKVQDSYSIRCAPQVHGAVRDALHHAVEVLTTELNSSTDNPLIFPDERETISAGNFHG
                     EPVALVMDYLAAAASELGNISERRIEQMVNPVFSQLPAFLVKDKGLHSGFMIAQVAAA
                     GLVSESKSLSFPASVDSIPTSAGKEDHVSMGTIAARKCAMVAENTACVLALEVLIACQ
                     ALDLRKPLKTSKALQAVHRKVRGAVPFMDTDRFLHPDFAKVRSLERELVAVAEKAAGP
                     LV"
     gene            complement(3249..5282)
                     /locus_tag="PKH10_02805"
     CDS             complement(3249..5282)
                     /locus_tag="PKH10_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNT27085.1"
                     /translation="MTSGRSFLSGLRLSDTQREAVTATDGPLLVFAGAGSGKTRVITC
                     RIAYLLGEKRVPPEEILAVTFTNKAAREMKERVASIVNDPSVTRRLTVCTFHALGLQI
                     LKREHKAIGFPAHFVVFSPYEQVELMKKVMEEEKISREQYSPYAVMSVVSRLKNDPDL
                     FNDQRFYLGNIRHGVARRLYEPYQRALKAAAAMDFDDLIMKPLDLFEQSEEIRKRYAG
                     KWRYLMVDEYQDTNRAQYRLIRELSSVHGNVCVVGDDDQSIYSWRGARVENILEFEED
                     FPGTRVVKLEENYRSVAEIVETAGRLIGFNRTRAEKKVFARRRAEEEKPITIHSVMDE
                     AEEADRVAREIASLSAAGLRYTDLAVMVRTNAQTLPFEKAFARQRIPYRVIGGQKFFE
                     NKEIKDLVAYLRILVRPDDEISLRRIINYPTRGIGTVAVEKLFEIAAVERSPALSVAE
                     RIGEYALLSPAQRDAIAKFSALYRGLVDEMACREPVPFAEHLIRTIGIEEAIHRSGES
                     DESVAIRLENIREFVSAVSYHHAERPAAGPREFWLDLINAVALIQSGDEDDKKKGVNI
                     ITAHSAKGLEFEVVFVPGFYQGGMPNRLALEEGNIEEERRLCYVAFTRARRRLIITIP
                     RMIKVRGRTLPTTASVFLSEAGLSMMSAAERREMAENYLQRIREKIEQDRPEE"
     gene            complement(5311..5760)
                     /locus_tag="PKH10_02810"
     CDS             complement(5311..5760)
                     /locus_tag="PKH10_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HNT27086.1"
                     /translation="MLSDLGSFGPIICLGLAACGSAIGCGLAGMTSHGVMSKTDEGHG
                     LFVALSAMPSSQTIYGFVLMILMSGKLSQLQDAGKPTAALFAIGVACGVALMLSAIYQ
                     GKACSSAILAVAKKPSIAGMSFAAPGIVESFAIFAMVGGIVLLQGLK"
     gene            5924..6502
                     /locus_tag="PKH10_02815"
     CDS             5924..6502
                     /locus_tag="PKH10_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT27087.1"
                     /translation="MRIFFSFFVLSLCFSLSLSCKKTPPDPALGQELLSALERGDESA
                     ARELIGRGADINARQAGTGYTPYVVAAKKCLTDPVRLLDKKGADMAVEVSSGSPVDRS
                     AFMNAAMAGCLDTIRYFVEEKKMDINTRHHVWNETILHKAVQGRDLTLARYAIERGVD
                     LNITDNNKRTALDLAKELGVVDITMLIENARK"
     gene            complement(6509..6895)
                     /locus_tag="PKH10_02820"
     CDS             complement(6509..6895)
                     /locus_tag="PKH10_02820"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT27088.1"
                     /translation="MIKRMLSRVNFEIAAQISHNGVDHPCAVQNLSLSGMYCVGGPDL
                     PMDSEVDVKLGLSSEHSAVSVAVKGKVARKDGDGQAFTFPHLDLDAFIFLRNIMVYNS
                     GNSDLIDKEYRDYLIWKSDKNEYIPG"
     gene            7018..7659
                     /locus_tag="PKH10_02825"
     CDS             7018..7659
                     /locus_tag="PKH10_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNT27089.1"
                     /translation="MMKIVESATIPALFVEQPDAKEHLDDISGALAAGLGLTTIHRVR
                     QVHGDRIVEDASGDGDGLIITKRGTGALIRTADCFPVVLADKDRALAGIFHCGWRGVE
                     LRLAAKGTRRLKELGARSLQAALFPGIERCCFRIGSELKDRFNDAGIPLETRGADLFA
                     DLGAAIVDQLAGEGVGEIEEVTRCTACTPGLFSYRRDRDDRRHATIVWLPAGS"
     gene            7769..8518
                     /locus_tag="PKH10_02830"
     CDS             7769..8518
                     /locus_tag="PKH10_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNT27090.1"
                     /translation="MLKDNPRVIIPNFFTSLNLLIGFIVVWLAIGGQYDRAAWYILLC
                     VIMDKMDGTAARAFDACSSFGIEFDSFSDFFSFGVAPAILILSFFQGQFNGNMEAVPL
                     SIKGACAVYILLTAIRLAKFNCADKEDKDFFYGLTSTQAGAMVAVLFLVARKHELWLL
                     RDETVMSGILLLFGVLMVSTFKYRKFKKRSARWLNILQSFVFLFLALLVLFHQWPEFI
                     LAVSVLYFVGAVIQTHFERPQGTDPDHEPPA"
     gene            8515..>8751
                     /locus_tag="PKH10_02835"
     CDS             8515..>8751
                     /locus_tag="PKH10_02835"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNT27091.1"
                     /translation="MTSLPLRQGIDRFDPSTIGRGKVALIANHTAVDRAGRSIVEVLR
                     DRGVKLSCIFSLEHGFFPVAQDMEAVRKEQKVRNI"
BASE COUNT         1649 a         2736 c         2601 g         1765 t
ORIGIN      
        1 agcacatcgc gttggcgcac ggcggctcgg tgtcggtcga aagcgaaccg gggaaaggat
       61 ccactttttc tctacaccta cctctttcct gaattcttac gaatagatac acgaccgtta
      121 cggtacggtc gggtagaccc ctgaggttat cgcgccggca cccacgccgg tcaccatcac
      181 atactgcgac ccgccttgcg acacctccac cgtcaccgcc gcgttcgcga tgatgagttg
      241 cgggctcgta ccgtgggtgt aagcgttggc ggtcgcgaaa acaaagccgg tcccgagccg
      301 cggcaccacc cggtcgagca ccgcctgcgc cggcgaggtg acatagatgc cggtcgtccc
      361 gaccaccgca ttacggccct gtccgttgtc cgggaacagc caatcgaccc actcctgcgc
      421 cgtcgaagag gcgagtccgt ggtgtgaaag atgcagaaca tcggcggcgc ccgacggaag
      481 caattcggcg ccccccggcg ttatggtaag cccggccgag tgcgacgcga tgaagccctc
      541 tatcttgata tcgcggccct gcgtgtcgcc ggcgaaaaga taattgaaat cgccccactt
      601 gatgacgccg ccgagactcc gcgcattctc ctgatcggtc tcgccgtagc cgatggtgat
      661 gccgttattt tctgccgata tcgcgccgcc ggcctgcgcc acccagccgc tggcgtgggt
      721 gaagtacagt ttcgcgccgt taccgagcgg gatgtaggag agcgcccccg ccgcatcgtc
      781 gtcgctcata tcgaggttgc cgagaaggag gccgggacag tcggaggtcg ggaagcgact
      841 gctcgcatcg ccgcacggca tctcggccac gccggtgcag agcgttacca tctgcgccgc
      901 aagttccccc cgcgccaagg tacagaattc attgaagtcc tcgacccccg gcatatcggc
      961 gccgatgtca tagaagcccc ggaggatcat cccttttcgc acctcgaggt gctggttcgc
     1021 tccggcgccg aagaggttgt caaagccgcc gatatggtcg ttgtggtagt gggtgatgat
     1081 cgcccagtct atcgccgcct cccctttccg ccgcgtcacc gcttcgagta tctgcggacc
     1141 atgaccgtcg ttggcgacat cgatgaggac cgtcgtcccg tcgggaccga cgataagagc
     1201 cgcctctccc atgttcatcc cgggcaggta tatctgttcg atgatgagtt cgcccaccgg
     1261 gatcacatcg tcgggctgga cggtgtcctc gtcaggcgcc gtgtccgccg atccgtccgg
     1321 tccctcaaga tcgacggtct cgtcgagcag gtcggggctt tcttccccct cgaccatttc
     1381 gtccttttcg ttgcctatgt cattttcgtc ctttgtctct tcgtccatcg ccgtgtcatc
     1441 agcgaccttc gtgtcggtga ccgccatctc atcgacggcc gtttcctccg gtgtactgca
     1501 cgacagcaca aaaagaaaag agcaaccaaa gaacaacgac caaagacggg gcatgccgac
     1561 ctcctgcgag taagtttttt cggatgatac cggaagagag gcgttttctc aatttttctt
     1621 cccagtaacg cattcctttc tttgctattt cttgattttg tgcgacgggg cactagactc
     1681 gcggcggata ctgtgaacgg aggcctccat gccgctgaaa aaactgctca tcgacggcga
     1741 aacgctgacc atccgcgatg tgtacgaggt cgcctgctgc gaccgccccg tcggcgtcgc
     1801 ccccgcggcg gcgaagaagg tcgagaagtg ccgccgcttc gtcgacaaac tgctcacgca
     1861 ggataaaccg gtctacggca tcaataccgg cttcgggata ctcagtgaag tggcgatccc
     1921 gaaagatcac ctcgacgatc tccagatgaa cctcatccgt tcccacgcca tcggcatcgg
     1981 cgacccgttc gaccgggcga ccgtccgcgc catcatgcta ctgcgcgcta atgtgctggc
     2041 caaaggcttc tccggcgtgc ggctctcgac gctcgagaaa ctggtcgagt tcatcaacgc
     2101 cgacatcgtc ccctacatcc cgcagaaagg atcggtcggc gcgtcgggcg accttgctcc
     2161 cctctcccac ctcgccctcg ccctgtgcgg cgaaggctac tgcctcggtg acgatatgaa
     2221 accggtcgat accgccggtg tgctcaaaaa gaaaaaagtg accccgatag cgctcaaggc
     2281 gaaagagggg ctcgccctca tcaacgggac gcaggtgatg accgccgtgg gggcgctcac
     2341 cgtcgagcgg ctcttcgggc tctcaaaact cgccgacatc tccctggcgc tcaccgtcga
     2401 cgcgatgctc gggaccgatc gcgcctttga cgagaaggtg agccgcgtcc gcgcccatcc
     2461 gggacagttc gaggtgtcgc gtaatgtccg aaaactcatc gccggttcga ccatccgcga
     2521 gtcgcaccgc tgttgcacga aggtgcagga ttcctacagt atccgctgcg cgccgcaggt
     2581 ccacggcgcg gtccgcgacg ccttgcacca cgccgtcgag gtgctcacga ccgagctgaa
     2641 ttcctcgacc gacaacccgc tcattttccc cgacgaaagg gagaccatat cggccggcaa
     2701 cttccacggc gaaccggtgg ctctcgtgat ggattatctc gccgccgccg cgagcgaatt
     2761 gggcaacatc tccgagcggc gcatcgagca gatggtcaat ccggtcttct cacaactgcc
     2821 ggcgttcctc gtgaaggaca agggactcca ctcggggttc atgatagcgc aggtcgcggc
     2881 ggcaggtctc gtgtctgaat cgaaatctct ctcgttcccc gcatcggtcg attcgatacc
     2941 gacctcggcg ggcaaggagg atcatgtgag catggggacg atagcggcgc gcaagtgtgc
     3001 catggtcgcc gagaacaccg cctgcgtgct cgccctcgag gtgcttatcg cctgtcaggc
     3061 cctcgatctc agaaagcctc tgaagacgag caaggcgcta caggcggtcc accggaaggt
     3121 gcggggcgcg gtccccttca tggataccga tcgtttcctg catcccgact tcgcgaaggt
     3181 gcggtcactc gaacgagaac tggtcgccgt cgccgagaag gccgccggcc cgctcgtttg
     3241 acgatcgatc attcctccgg ccggtcctgc tctatcttct cgcgaatgcg ttggaggtag
     3301 ttctccgcca tctcgcgccg ttcggcggcg ctcatcattg aaagccccgc ctccgacagg
     3361 aacaccgacg cggtggtcgg cagggtcctg ccacgcacct ttatcatgcg cggtatcgtg
     3421 atgatcagac ggcggcgggc ccgcgtgaag gcgacatagc agagacgccg ttcctcctcg
     3481 atgttcccct cctccagcgc gagacggttg ggcatgccgc cctgataaaa gccggggacg
     3541 aagaccacct cgaactcgag ccccttggcg ctgtgggccg tgatgatgtt gacccccttc
     3601 ttcttgtcgt cctcgtcgcc tgactggatg agcgccaccg cgttgatgag gtcgagccag
     3661 aactcgcgcg gacctgcggc gggccgttcg gcgtggtgat aggaaacggc gctcacgaac
     3721 tcgcggatgt tctcgagccg tatagcgacc gattcgtcgc tctcgccgct ccggtgtatc
     3781 gcttcttcga tgccgatcgt ccggataagg tgttcagcga aggggaccgg ttcacgacag
     3841 gccatctcgt cgaccaaccc gcgatacagg gcgctgaact tcgctatcgc gtcgcgctgg
     3901 gccggcgaga gcagggcata ctcccctatc cgttcggcca ctgagagggc tggcgacctc
     3961 tcgaccgccg cgatctcgaa cagtttttcg accgcgacgg tgccgatacc acgggtcgga
     4021 tagttgatga tgcggcgcaa acttatctcg tcgtcaggcc gcacgaggat gcgcaggtag
     4081 gcgacaaggt cctttatttc cttgttctcg aagaacttct gcccgccgat gacccgatac
     4141 gggatgcgtt gccgcgcgaa cgccttttcg aacggtaggg tctgcgcgtt ggtgcgcacc
     4201 atcaccgcaa gatcggtgta gcgcaggccg gccgccgaaa gcgacgctat ctcgcgggcg
     4261 acccgatcgg cctcttccgc ttcgtccatg accgagtgaa tagttatcgg cttctcctcc
     4321 tcggcgcgtc ggcgagcgaa caccttcttt tcggcccggg tccggttgaa cccgatgagc
     4381 cgccccgctg tctcgacgat ctcggcgacc gaccggtaat tctcctccag tttcacgacg
     4441 cgggtgccgg gaaaatcctc ttcgaactcg aggatgttct cgacccgcgc gccgcgccaa
     4501 ctgtaaatgc tctgatcgtc atcgcccacc acacacacat tgccgtggac cgacgaaagt
     4561 tcacggatga gccggtattg tgcgcggttg gtgtcctgat actcgtccac catgaggtat
     4621 cgccattttc cggcatatct tttccgtatc tcctcgctct gctcgaacag atcgagcggt
     4681 ttcatgatga gatcgtcgaa atccatcgcc gcggccgcct tgagcgcccg ctggtacggc
     4741 tcgtaaagcc gacgtgcgac accgtgccgt atgttgccga ggtagaatcg ctggtcgttg
     4801 aaaaggtcgg ggtcgttctt gagccgcgag acgaccgaca tcaccgcgta aggcgagtac
     4861 tgttcgcgcg atatcttctc ttcctccatc accttcttca tcagttcgac ctgctcatag
     4921 ggcgagaaga cgacgaagtg ggccgggaaa ccgatcgcct tgtgctcccg tttgagtatc
     4981 tggagaccga gcgcgtggaa ggtgcagacg gtgagccgcc gcgtgaccga cgggtcattc
     5041 actatcgagg ccacccgctc cttcatctcg cgcgccgcct tattggtgaa ggtgacggcg
     5101 aggatctcct cgggcgggac cctcttctcc ccgagcaggt aggcgatgcg gcaggttatg
     5161 acgcgggtct tgcccgatcc ggcacccgcg aagacgagga gcggcccgtc ggtggcggtg
     5221 accgcctcac gctgggtgtc gctcaggcgc agaccggaga ggaaggagcg accggaggtc
     5281 atggcgcgcg ccgcgtgacc cgggcggtgg ctacttgagg ccctgcaaaa ggacgatgcc
     5341 gccgaccatc gcgaagatcg cgaatgactc gacgatgccg ggagcggcga aggacatgcc
     5401 ggcgatcgag ggcttcttgg cgaccgcaag gatggccgag gaacaggcct taccttggta
     5461 gatggccgaa agcatgagcg cgacgccgca ggcgacgccg atggcgaaca acgccgcggt
     5521 cggcttgccc gcatcctgca gttgcgagag tttgccgctc atgaggatca tcaggacgaa
     5581 cccgtagatg gtctgcgaag acggcatcgc cgaaagcgcg acgaacaggc cgtgcccttc
     5641 gtcggtcttc gacatgacac cgtgcgaggt catgccggcg agaccacatc cgatggcgct
     5701 tccgcaggcg gcaagaccga gacagatgat gggaccgaat gaaccgagat ccgataacat
     5761 gccttcctcc ttaagtatct tcgtttgtgc caaaagaccc gttgacgagc cgagcatact
     5821 cggaaacacg ataaaagcaa aaaagggccg gcgaccgttc ttcttgcttt tcctcaggac
     5881 tcttctatca tcggaccgtt acgatgaccg atgaggtgcg ctcatgagga tctttttttc
     5941 gttttttgtt ctatcgctct gtttttccct gtcgctctca tgcaaaaaga cgcctcccga
     6001 cccggcgctc ggacaggaac tcctctcggc cctcgaacgg ggcgacgagt cggcggcgcg
     6061 ggaactcatc gggcgcggcg ccgacatcaa tgcgcggcaa gccgggaccg ggtacacccc
     6121 ctatgtcgtc gcggcgaaga aatgccttac cgatccggtg cgtctgctcg acaagaaggg
     6181 ggcagatatg gccgttgagg tctcgtcggg aagtccggtg gatcgatcgg ccttcatgaa
     6241 tgccgccatg gcgggatgtc tcgacacgat ccgttatttc gtcgaggaga aaaagatgga
     6301 catcaatacg cggcaccatg tctggaacga aacgatcctt cataaggccg tgcaggggcg
     6361 cgatctgacg ctcgcccgct acgccattga acgcggagtg gaccttaaca tcaccgacaa
     6421 caacaagagg acggccctcg acctggcgaa ggaactcggc gtcgttgaca tcacaatgct
     6481 tattgagaac gcgcggaagt aggcgggctc aaccggggat gtattcgttc ttgtccgatt
     6541 tccagatgag atagtcacgg tactccttat cgatgagatc ggagttcccg ctgttgtaga
     6601 ccatgatgtt acgcaggaag atgaaggcgt cgagatcgag gtgaggaaag gtgaaggcct
     6661 gaccgtcgcc gtccttgcgg gccaccttac ccttcaccgc gacgctgacc gccgaatgtt
     6721 ccgagctgag ccccagtttg acatctacct ccgaatccat cggaagatcg gggccgccga
     6781 cgcaatacat gccggaaaga ctgaggttct gcaccgcgca gggatggtcg acaccgttgt
     6841 ggctgatctg cgccgctatc tcgaaattga cgcgtgaaag catccgtttg atcatatcgc
     6901 ttgcctgtag cacaccataa cgcgaagcac tatacgaacc ggtgccgctt tgtcaacaga
     6961 aagtgtttga caagtgccac aacggttgat acggtcgcgc ggaccaccac ggggagcatg
     7021 atgaaaatag tggagtcagc gacgatcccg gccctgttcg tcgaacagcc cgacgcgaaa
     7081 gagcatctcg atgacatttc cggtgctctg gccgccggac tcgggctcac gacgatacac
     7141 cgcgttcgcc aggtgcatgg tgaccgtatc gtcgaggatg cttcgggcga tggtgacggg
     7201 cttatcatca cgaaacgcgg caccggtgcg ctcatccgga ccgccgactg tttcccggtc
     7261 gttctcgccg acaaggaccg ggcgctcgcc ggcatctttc actgcggctg gcgtggcgtt
     7321 gaattgcgtc tcgccgcgaa ggggacgcgt cgtttgaagg aactcggcgc ccgttccctg
     7381 caggccgcgc tgttccccgg catcgagaga tgttgtttcc ggataggttc cgaactgaag
     7441 gaccgtttca acgacgccgg gataccgctg gagacgcgcg gcgcagacct ttttgccgat
     7501 ctcggggcgg ccatcgtcga tcaactggct ggagaaggcg tcggcgagat agaggaggtg
     7561 acacgctgca ccgcgtgcac cccgggactg ttctcctacc ggcgcgatcg cgacgaccgg
     7621 cgccatgcga ccatcgtctg gctccccgcg ggctcgtagc ccttttttgt cgaaccggat
     7681 atctccgggg aaaagagctc gcttccttgc attttttttc tcgtgaacat ataagggtgg
     7741 ctgggaacct tacttttgag gggcaggcat gctgaaagat aatccgcggg tcatcatccc
     7801 gaatttcttc acgagtctca acctgctgat agggttcatc gtcgtctggc tcgccatcgg
     7861 cggccaatac gaccgcgcgg catggtatat cctgctgtgc gtcatcatgg acaagatgga
     7921 cggtaccgcg gcgcgcgctt tcgacgcctg ctcgagtttc ggcatcgagt tcgactcgtt
     7981 cagcgacttc ttctcgttcg gcgtcgctcc ggcgatactc atcctttcct tcttccaagg
     8041 gcagttcaac ggcaacatgg aggcggtgcc gctttcgata aagggggcct gcgccgtcta
     8101 catccttctc acggcgatac ggcttgccaa gttcaactgc gccgacaagg aggacaagga
     8161 tttcttctac gggctcacct cgacgcaagc gggggcgatg gtcgccgttc tgttcctcgt
     8221 ggcccgtaaa cacgaacttt ggctcctgcg ggacgaaacc gtcatgagcg gcatcctgct
     8281 tctgttcggt gtccttatgg tctcgacctt caagtaccgc aaattcaaga aaaggtcggc
     8341 tcgctggctc aatatcctgc agagtttcgt tttcttattc ctcgctctgc tcgtactttt
     8401 ccatcagtgg cccgaattca ttctcgcggt atcggtgctc tacttcgtcg gcgccgtgat
     8461 acagacccac ttcgagcgac cgcaggggac cgatcccgac cacgagcccc ccgcatgacc
     8521 tcgcttccgt tgcgacaggg gatcgaccgg ttcgacccct cgacgatcgg tcgcggcaag
     8581 gtcgcgttga tagccaacca caccgcagtg gatcgcgcgg gtcggtcgat cgtcgaggtg
     8641 ctccgcgacc gcggcgtcaa actgtcctgt atcttctcgc tggaacacgg cttcttcccc
     8701 gtcgcgcagg acatggaagc ggtgcggaag gagcagaagg ttcgcaacat c
//
LOCUS       DAOQUE010000055         8727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8950_length_8727_cov_4.764645, whole genome shotgun sequence.
ACCESSION   DAOQUE010000055 DAOQUE010000000
VERSION     DAOQUE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8950_length_8727_cov_4.764645"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKH10_02840"
     CDS             <1..356
                     /locus_tag="PKH10_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HNT27092.1"
                     /translation="NSVPYEDLRKKGGGEPSGKLRERIERAGTAQAERFRGKPNLVNA
                     RMAPADIEKFCVLAPAAETLLRAAIEKMGISTRGYAKLLKVSRTIADLAGAEHIDTPH
                     IAEAIQYYNRSVFNQ"
     gene            469..930
                     /locus_tag="PKH10_02845"
     CDS             469..930
                     /locus_tag="PKH10_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27093.1"
                     /translation="MKKYLVLMTVALVFAIVSAQTAPAPAKPAPAPTAAKPAPAKPAP
                     AAAATVAPAPAKVAPAPVAEVAAKPADLKKAQKAEMAELDKAQKTAKEELTKKQKEEQ
                     EAFEAEYKAKKEEMKKRHADEKKAMKETQDAEKKAMQEKHKAALDALKATK"
     gene            complement(1107..2489)
                     /gene="rlmD"
                     /locus_tag="PKH10_02850"
     CDS             complement(1107..2489)
                     /gene="rlmD"
                     /locus_tag="PKH10_02850"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNT27094.1"
                     /translation="MQKDRSQTRTVTVTVDAQDPSGAGAIHAFGKKILVTGIIAGERV
                     ALHIKDPREIYPEAELRRIVARSPHRVDPRCPVFGRCGGCTLQMLAYPEQLKWKRRVV
                     KDALGKMLEDRSVLVRPTIGMKEPWLYRNKVQYPVGRDGRFPIIGFYAPVSYRIVTNR
                     GCPIQHPEADLLKEALLAHLKEHRIEPYDERTGDGLVRTLMTRKGFATGQVVCILVLN
                     GRTLPAMEGFVAAARAAVPALTGVVVNVNTKKGRVILGRENRVVWGSGTLTDTLLGHT
                     FEISPNSFYQVNHAQAESLYRAALELAAIEKKDVVFDLYAGAGTITIPAAALAKEAWG
                     VELSPEAVADAERNAKRNGMENARFVAGDVVKETSRLIEKKIRPDIVILDPPRAGCDK
                     PLLLMLAAARPKRIVYVSCDPRTLARDLGILELHGYRVAEVQPVDLFPHTGHVEAVAL
                     LEPMGKRRGS"
     gene            2622..3383
                     /locus_tag="PKH10_02855"
     CDS             2622..3383
                     /locus_tag="PKH10_02855"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNT27095.1"
                     /translation="MFFCVDIGNTNTVVGITDHGKITARWRVETDRHATFDDLTAKFH
                     PLLTLAGIPREKVEKVIVSSVVPAWDHSWERFSREYLGREALFVGAGTRTGITVAVDS
                     PQEVGADRIVNAAAAIARHPGGALIVDSGTAITIDAVSPDHRYLGGAIMPGMLISIEA
                     LAARTAKLPRVHLDRPERAVGRSTAGGIQSGVYYGFAGMVDRVIEEMAAELDFTPRVI
                     ATGGLAGGLAAVSRRIEEYDRDLTLYGLDLIAAMN"
     gene            3393..4658
                     /locus_tag="PKH10_02860"
     CDS             3393..4658
                     /locus_tag="PKH10_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27096.1"
                     /translation="MWRRAQDPVQKKAILDQFLPLTRDDYVSAVIAFADDENDEVRSL
                     AFEKLKALDHAVLIKRVRPDMSRATVRTLARLCVADPNPQLIAALVNSKRLTWPVIKE
                     RWLSPNEELWRALIVNRSFILLSHPDREAVLAFLKTFSASLTAAYREQLGWVTPKELK
                     EFVVPSDEEPRPPEPPAPVKAPADKSAPVTPAGPVPPVTPIDAPEGDRLLIKGLDSFE
                     EVAEPSREDAVAALDEGQEINISGALFDTGQGVVTEDDEVVDLDSIDLEVPDFLLVED
                     PFAGMSKEEIAEHRKNIADIIDKLTMPQRMKLATVGNMEARKLLIKDPRRMVAMAVLS
                     NGGITPGEIAALAGNPATMQDVVEYIASSRNLCKNYMVKVALVTNPKTPIKTGMLFLD
                     VIRRADLKKIAESKNISPIIRNMAKKRIH"
     gene            4665..5849
                     /gene="xseA"
                     /locus_tag="PKH10_02865"
     CDS             4665..5849
                     /gene="xseA"
                     /locus_tag="PKH10_02865"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNT27097.1"
                     /translation="MDDRILSVSELVRALKGVVESNFGVVILRGEVSNLHTSLSGHTY
                     FTLKDETAQVRCALFKGQKAGDRTLVEDAAYLVWGRVSLYEARADITLIVSFFLPAGE
                     GTEALRIQRLKDKLQKQGLFDPARKKELPPLIEHIGVVTAAGGAAIRDIIRVGRDRYP
                     RLKITLYPALVQGDQAEEMLARGVEALQGIPGIEAIIVGRGGGAKEDLRAFNGERLAL
                     AVASCRVPVVAAVGHETDRTILDLVASHAVPTPSAAAELVTRPAADLLAEATGRAALM
                     GEQVRALLRDRQMRLDRLLLQIPRPAEWLQRLRLRLLGVSAALAAELRRIHRDAERRL
                     ADAVLRVEMRSPLRPLEQGYALIRQGGAAVGRLAALDGDRPFAVRFLDGETTAVVEKK
                     RS"
     gene            complement(5866..>8727)
                     /locus_tag="PKH10_02870"
     CDS             complement(5866..>8727)
                     /locus_tag="PKH10_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNT27098.1"
                     /translation="EGLVRFDGIRFVTYTEENTPAFASRHIMSLFVDHRGTLWIGTYN
                     GLVRHEAGRFSRLEVTPLPTAVFGLAGDRRGTLWIAAANGLFSLKDDLVTRYSVDDGF
                     PSDEMWSLAIDREQAIWVSSGRINLCRFTPDTRPACHAYPDLITKVYLDRRQRLWIGT
                     YAGSRLHQEPGPVVQYISPGVPLYAEAMHEDRDGNLWIGSREQELVKRSPRDRIFRND
                     VLHDPIGPVKSIFEDRERNLWVGTGGNGLHRFSDSRIAVYGQRNGLSTDIVLPVQRTA
                     SSGVVFGTYDGKLYRLNENELEPVPLPGVPPIMLALTLIEDRNGLLWVGTRGQGLIQV
                     PRHGPAREFKIPSEVRSVAALYEDRSGAIWMALRGDALLRWDGERFERFDDPKDFFAN
                     VHTIRESPGGDLWFASGAAGLAMRRGDTFIFGDSLFRDALPSRAIQTLHPDSRGLLWI
                     STNGSGLFIWNGERITPVKVPEGMSVSLVYNIIEDHIANLWMSTNRGILRLERSEVDL
                     YLRGSIPHLSPLVFTTSHGLKSAECVGGVQQTADRGSDGKLYFATVKGLAVIDPDNLP
                     TNIIRPPVRIEQVVVDNRPLSPAELEAPDLEKGIARLEVHYTALSFINPSYITFKYKL
                     EGFDPFWVSVGNNRDAYYTNLRPGDYTFRVIAANSDGIWNEEGASFSFRIRPLFYQTV
                     WFYFFLTGSVLLCGYLIFLLRVRAIRAQNRRLSSLVEEKTRDLTNVNERLREMSLIDP
                     LTGLRNRRYFSDILKAEAASYLRARTRVASGLDQRNIDQDKVWGVFLADADYFKQVND
                     RFGHDSGDLILTQMATLFKNAVRADDVVIRWGGEEFLVVLRNSVPDYLPAFAEKLRTL
                     VQNSFLRTAGGDLIRKTVSIGFVPLPFYRTDPERITIEQAVSIADLGLYYAKQNGRNR
                     SVHIEAGGRIADTAPEEMQKMLADLDASVRNGFFSIAEK"
BASE COUNT         1582 a         2767 c         2871 g         1507 t
ORIGIN      
        1 tcaactcggt cccgtacgag gacctgcgca aaaaaggggg cggcgaaccg tcgggtaagt
       61 tgcgcgagcg catcgagagg gcgggcacgg cgcaggcgga acggttccgc ggcaaaccga
      121 acctcgtgaa cgcccggatg gcccccgccg acatcgagaa gttctgcgtg cttgccccgg
      181 cggccgagac gctcctacgc gccgcgatag agaagatggg catctcgacg cgcgggtacg
      241 ccaaactact caaggtctcg cgtaccatcg ccgatcttgc gggggccgag catatcgaca
      301 ccccgcacat cgccgaagcg atacagtact acaaccgctc ggtgttcaat caataaggaa
      361 cggtgtgttt tcctttcgga taaaaatttg ccttttcaag aggtaaggca taccatcgtc
      421 cccgatgagc gttccgtttc aaaccttaga ccgatcaggg aggaatccat gaaaaagtac
      481 ctcgttctca tgaccgttgc gctcgtgttc gcgatcgttt cggcgcagac cgctccggcc
      541 ccggcgaagc ccgctccggc cccgaccgcc gcgaagcccg ctccggcgaa gcccgctcca
      601 gccgctgcgg ctaccgttgc cccggccccg gcaaaggtcg ctccggctcc cgtcgctgag
      661 gtcgccgcga agccggccga tctcaagaag gcgcagaaag ccgagatggc cgaactcgac
      721 aaagcgcaaa agaccgccaa ggaggaactc accaagaaac agaaagagga acaggaggca
      781 ttcgaggccg aatacaaggc gaaaaaagaa gagatgaaaa agcggcatgc cgacgagaaa
      841 aaagcgatga aagaaacgca ggatgccgaa aagaaggcaa tgcaagaaaa gcacaaggcg
      901 gcgctcgacg ccctcaaggc gacgaaatag tccctgtttc aaatcccgtc aaggcttcct
      961 cgcatgaatg ctccggcttc cggcctgacg acacggtgct acagtttcgt ctaattcggc
     1021 cttcagtgac aaggggccgg aaggtataaa atcccggtga acgacccttc gtcccccgtg
     1081 tcaaaggggg atggaagggg gatttttcac gatccccttc tttttcccat cggttccagc
     1141 agcgccaccg cctcgacgtg gccggtgtgc gggaacaggt cgaccggctg cacctcggcg
     1201 acgcggtagc cgtgcagttc gaggatgccc agatcgcggg cgagcgtccg ggggtcgcag
     1261 gaaacataga cgatccgttt gggacgcgcc gcggcgagca tgaggaggag cggtttgtcg
     1321 cacccggcgc gcggcgggtc gagtatgacg atgtcgggcc ggatcttttt ctcgatgagc
     1381 cgcgaggtct ccttcacgac atcgccggcg acgaaccgcg cgttctccat cccgttgcgt
     1441 ttcgcgttgc gttccgcgtc ggcgaccgcc tcgggcgaca attccacccc ccatgcctcc
     1501 ttggcgagag cggcggccgg tatcgtgatg gtccccgctc cggcgtaaag gtcgaacacg
     1561 acatcctttt tttctatcgc ggccagttcg agcgcggcgc ggtacagcga ctcggcctgc
     1621 gcgtggttga cctgatagaa cgagttgggc gatatctcga aggtgtggcc gagcagggta
     1681 tcggtcagcg tgccggaacc ccagacgacg cggttctcgc ggccgagtat gacgcgcccc
     1741 ttcttcgtgt tgacattgac gaccacgccg gtgagcgccg gtaccgccgc gcgggccgcg
     1801 gcgacgaagc cttccatcgc cgggagcgtc ctgccgttca gcacgaggat gcagacgacc
     1861 tgtcccgtcg cgaagccctt gcgggtcatg agcgtccgga cgagcccgtc gccggtccgc
     1921 tcgtcgtacg gctcgatgcg atgttccttg agatgggcga gcagggcctc cttcagcagg
     1981 tcggcctcgg gatgctggat gggacagccg cggttggtga cgatgcggta actgaccggc
     2041 gcgtagaacc cgatgatggg gaacctgccg tcgcggccga ccggatactg caccttgttg
     2101 cggtagagcc acggctcctt catcccgatc gtcggccgga cgagcaccga ccggtcctcg
     2161 agcatcttgc cgagcgcatc cttcacgacg cgccgtttcc atttcaactg ctcgggatac
     2221 gcgagcatct ggagcgtaca gccgccgcaa cgaccgaaga cgggacagcg cggatcgacg
     2281 cggtgcggcg accgcgcgac gatgcgccgt agctccgcct cggggtatat ctcgcgcggg
     2341 tccttgatgt ggagcgcgac ccgctcgccg gcgatgatgc cggtgaccag tatcttcttg
     2401 ccgaaggcgt ggatggcccc cgcgcccgac gggtcctgcg cgtcgaccgt gaccgtgacg
     2461 gtcctcgtct gcgagcggtc cttttgcatt cctgtctctc cggttgaccg gaaggcgtta
     2521 tcgtcaggaa cggcgctcgt gtcaaggatt cacgccggtt tcttgccttt tccccttccc
     2581 gcgttatagt ggggcatgac cgcgaactgg cgagggaccg catgttcttc tgcgtggata
     2641 tcggcaacac caacaccgtc gtcggcatca ccgaccacgg caagataacg gcgcgctggc
     2701 gcgtcgagac cgaccgccac gccaccttcg acgatctcac ggcgaaattc cacccgctcc
     2761 tgacgctcgc cggcatcccg cgggagaagg tcgagaaggt catcgtcagt tcggtcgtcc
     2821 cggcgtggga ccatagttgg gagcggttct cgcgcgagta tctgggtcgc gaggcgctct
     2881 ttgtcggcgc ggggacgcgg accgggataa cggtcgcggt ggacagcccg caggaggtcg
     2941 gcgccgaccg catcgtcaac gcggccgccg ccatcgcgcg tcaccccggc ggggcgctca
     3001 tcgtcgattc gggcaccgcg ataacgatag acgcggtctc tcccgaccat cgctatctgg
     3061 gcggcgccat catgccgggg atgctcattt ccatcgaagc tctcgccgcg cggaccgcca
     3121 aactgccgcg ggtccatctc gaccgccccg aacgggccgt cggacgcagc accgcaggcg
     3181 gcatccagag cggcgtctat tacgggttcg ccggcatggt ggaccgcgtg atagaagaaa
     3241 tggcggccga actcgatttc acgccgcgcg tcatcgcgac cggcggtttg gccggcgggc
     3301 tcgcggcggt gtcgcgccgc atcgaggagt acgaccgcga ccttacgctg tacgggctcg
     3361 atctcatcgc ggcgatgaac taggagaacg atatgtggcg ccgggcgcag gatcccgttc
     3421 agaaaaaggc gatactcgat cagttcctcc cgcttacccg cgatgactat gtgagcgcgg
     3481 tcatcgcctt tgccgatgac gagaacgacg aggtccgctc gcttgctttc gagaaattga
     3541 aggcgctgga ccacgccgtc ctgatcaagc gggtccgccc cgatatgtcg cgggccaccg
     3601 tccggacgct tgcgcggctc tgcgtcgccg acccgaatcc gcaactcatc gccgcgctcg
     3661 tgaactccaa gcggctgacc tggccggtga taaaggagcg gtggctgtcg ccgaacgagg
     3721 aactgtggcg ggccctcatc gtcaaccggt cgttcatcct gctctcccac ccggaccgcg
     3781 aggcggtgct cgcgttcctg aagaccttca gtgcgtcact caccgccgcc tatcgcgagc
     3841 aactcggctg ggtgaccccc aaggaactca aggagttcgt cgtcccgtcg gatgaggagc
     3901 cgcgtccccc ggagccgccc gcccccgtta aggccccggc ggacaagtcc gcgccggtca
     3961 cgccggccgg gccggtgccg ccggtcacgc cgatcgacgc tccggaaggc gaccggctgc
     4021 tcatcaaggg actcgattcg ttcgaagagg tggccgaacc gtcgcgcgaa gatgccgtcg
     4081 ccgcgctcga tgaggggcag gagataaata tctcgggcgc cctgttcgac accggtcagg
     4141 gggtggtgac cgaggatgac gaggtcgtcg acctcgattc gatcgacctc gaggtgcccg
     4201 acttcctgct cgtcgaggat cccttcgccg ggatgtcgaa ggaagagata gcggaacacc
     4261 gcaagaacat agcggatatc atagacaaac tgacgatgcc gcaacggatg aaactcgcca
     4321 ccgtcggcaa tatggaggcg cggaaactgc tcatcaagga tccccgccgc atggtggcga
     4381 tggcggtgct cagcaacggc ggcatcacgc cgggcgagat cgccgcgctc gcggggaacc
     4441 cggccaccat gcaggatgtg gtcgaataca tcgccagcag ccgcaatctc tgcaagaact
     4501 acatggtgaa ggtcgcgctc gtgacgaacc ccaagacgcc cataaagacc ggcatgctgt
     4561 tcctcgatgt catccgccgc gccgatctca agaagatagc cgaaagcaag aacatctcgc
     4621 cgatcatccg gaacatggcg aaaaaacgga tacactgacc gcccatggac gaccgtatcc
     4681 tctcggtctc cgaactggtg cgcgccctca agggggtcgt cgagtcgaat ttcggcgtcg
     4741 tgatactgcg cggcgaggtg agcaacctgc acacctcctt atcgggacac acctacttca
     4801 cgctcaagga cgagacggcg caggtccgtt gcgcgttgtt caaggggcag aaagcgggcg
     4861 accggacgct cgtcgaggac gccgcctatc tcgtctgggg ccgcgtgtcg ctgtacgagg
     4921 cgcgcgccga tatcaccctc atcgtgagtt tcttcctgcc ggcgggcgag gggaccgagg
     4981 cgctgcggat acagagattg aaggacaaac tgcaaaaaca ggggctgttc gacccggcgc
     5041 gcaagaagga gttgccgccg ctcatcgagc atatcggcgt cgtgaccgcg gcgggcgggg
     5101 ccgccatccg cgacatcatc cgtgtcggtc gcgatcgtta cccgcgactc aagataacgc
     5161 tctatccggc gctcgtgcag ggggatcagg ccgaggagat gctggcgcgc ggcgtcgagg
     5221 cgttgcaggg gatccccggc atcgaggcga tcatcgtcgg tcgcggcggc ggcgcgaagg
     5281 aggatctccg cgcctttaac ggcgaacggc tggcgctcgc cgtggcgtcg tgccgcgtgc
     5341 cggtcgtcgc ggcggtcggt cacgagaccg accggacgat cctcgatctc gtcgcgagtc
     5401 atgcggtgcc caccccttcg gcggcggccg aactggtgac ccgccccgcg gccgatcttc
     5461 ttgccgaggc gacggggcgc gccgcgctga tgggcgaaca ggtccgggcg ctcctgcgcg
     5521 accggcagat gcggctcgat cgcctgctgt tgcagatacc gcgtcccgcc gaatggctcc
     5581 agcgcctgcg cctgcggctc ctcggtgtgt cggccgctct ggcggcggaa ctccggcgca
     5641 tccaccgcga cgccgagcgg cgactcgccg acgccgtgct ccgtgtggag atgcgcagtc
     5701 cgctccgacc gctcgaacag gggtatgcgc tcatccggca gggaggcgcc gccgtcggtc
     5761 ggctcgccgc gctcgatggc gatcgtccgt tcgcggtgcg gttccttgac ggcgaaacga
     5821 cggcggtcgt cgaaaagaaa aggtcgtgaa ggtccggccg ggtcgttatt tctcagcgat
     5881 ggagaagaac ccgttcctga ccgatgcgtc cagatcggcg agcatcttct gcatctcctc
     5941 gggcgcggtg tcggcgatgc ggccgcccgc ctcgatgtgg acggagcggt tacgcccgtt
     6001 ctgctttgcg taatagagcc cgagatcggc gatggaaacg gcctgttcga tcgttatccg
     6061 ctccgggtcg gtgcggtaga agggaagcgg aacgaagccg atggaaacgg tcttgcggat
     6121 gagatcgccg ccggcggtcc gcaggaacga attctgcacg agcgtccgca gtttctcggc
     6181 gaacgcgggg agatagtcgg gaacgctgtt ccggagcacg acgaggaact cctcgccgcc
     6241 ccagcggatg acgacatcgt cggcgcgcac ggcgttcttg aagagcgtcg ccatctgggt
     6301 cagtatcagg tcgcccgaat cgtgtccgaa ccggtcgttc acctgtttga aataatcggc
     6361 gtcggcgagg aacacgcccc acaccttgtc ctgatcgata ttgcgttggt cgaggccgct
     6421 cgcgacgcgc gtccgggccc gcaggtaact cgccgcctcc gccttgagga tgtcgctgaa
     6481 gtaccggcgg ttccgcagcc cggtgagcgg gtcgataagc gacatctcgc ggagccgctc
     6541 gttgacgttg gtgaggtcgc gtgtcttctc ctcgacgagc gacgagagcc gccggttctg
     6601 cgcccgtatc gccctgacgc ggagcaggaa tatcagatag ccgcagagca gtaccgaccc
     6661 ggtgaggaaa aagtagaacc atacggtctg atagaacagc ggccggatgc ggaaggaaaa
     6721 ggaagccccc tcctcgttcc agatgccgtc gctgttcgcg gcgatgacac ggaaggtgta
     6781 gtcgcccggt ctcagattcg tgtagtatgc atcgcggttg ttccccaccg acacccagaa
     6841 gggatcgaac ccctccagtt tgtacttgaa ggtgatataa ctcgggttga tgaaactgag
     6901 cgcagtgtag tgcacctcaa ggcgcgctat ccctttttca aggtcgggcg cctccaactc
     6961 ggcgggcgag agcggccggt tgtcgaccac cacctgctcg atgcggaccg gcggtcggat
     7021 gatgttggtc ggcaggttgt cggggtcgat gaccgcgagc cccttgaccg tcgcgaaata
     7081 gagttttccg tcgctcccgc ggtcggcggt ctgctggacg ccgccgacgc attcggcgct
     7141 cttgagcccg tgcgaggtgg tgaacacgag cggcgaaagg tgcggtatgc tgccgcgcag
     7201 gtagagatcg acctcgctcc gttcgagccg cagtatgccg cggttggtgc tcatccagag
     7261 gttggcgata tggtcctcga tgatgttgta taccagactc accgacatcc cctccgggac
     7321 cttgaccggc gtgatccgtt cgccgttcca gatgaacagg ccgctcccgt tggtcgatat
     7381 ccacaaaagc ccccgactgt cggggtgcag ggtctggatg gcgcgcgagg gaagcgcatc
     7441 ccggaagagc gagtcgccga agatgaaggt gtcgccgcgt cgcatcgcca gtccggccgc
     7501 cccgctcgcg aaccacaggt cgccgcccgg cgactcacgg atggtgtgaa cattggcgaa
     7561 gaagtccttc gggtcgtcga accgttcgaa tcgttcgccg tcccagcgca ggagcgcgtc
     7621 accgcggagc gccatccata tagcgccgct ccggtcctca tagagcgccg cgaccgaccg
     7681 gacctccgac ggtatcttga actcgcgcgc cggtccgtgt cgcggcacct gtatcaggcc
     7741 ctgtccgcgc gttcccaccc acaagagacc gttgcggtcc tcgatgagcg tgagagcgag
     7801 catgatcggc gggacgccgg ggagcggcac cggctcgagt tcgttctcgt tcaggcggta
     7861 cagtttgccg tcataggtgc cgaacacgac gcccgatgag gcggtccgct gtaccggtag
     7921 gacgatgtcg gtgctgagac cgttccgctg gccgtagacg gcgatgcggc tatcggagaa
     7981 ccggtgcaga ccgttgccgc cggtgccgac ccagaggttc cgctcacggt cctcgaatat
     8041 tgacttgacc ggcccgatcg ggtcatggag cacatcgttg cggaatatgc ggtctcgcgg
     8101 tgatctcttg accaattcct gctcgcgcga gccgatccag agattcccgt cgcggtcctc
     8161 atgcatcgct tcggcgtaca gcgggacgcc gggagagata tactggacga ccggaccggg
     8221 ctcctgatgc aggcggctcc cggcgtaggt gcctatccag aggcgttgtc gccggtcgag
     8281 gtacaccttg gtgatgaggt cgggataggc gtgacacgct ggtcttgtat cgggagtgaa
     8341 gcggcagagg ttgatccgcc ccgaggagac ccatatcgcc tgttcgcggt cgatcgcgag
     8401 cgaccacatc tcgtcgctcg ggaatccgtc gtcgacgctg tagcgggtca cgagatcgtc
     8461 cttgagcgag aacaggccgt tggccgccgc gatccagagt gtcccgcgcc ggtcgccagc
     8521 gaggccgaac accgccgtcg ggagcggcgt cacctccagt cgcgagaagc ggcccgcctc
     8581 atgacggacg agcccgttgt aggtgccgat ccacagcgtg ccccggtggt cgacgaacag
     8641 actcatgatg tgacgcgagg cgaaggcggg ggtgttctcc tcggtgtagg tgacgaagcg
     8701 gatgccgtcg aaccggacga gtccctc
//
LOCUS       DAOQUE010000056         8698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_8983_length_8698_cov_4.936712, whole genome shotgun sequence.
ACCESSION   DAOQUE010000056 DAOQUE010000000
VERSION     DAOQUE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8983_length_8698_cov_4.936712"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..722
                     /locus_tag="PKH10_02875"
     CDS             234..722
                     /locus_tag="PKH10_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27099.1"
                     /translation="MKKIVVLFFFLTLSISCYATSLADTLSRGYGTPVTYWYWYAWQG
                     EDGNFYNGGCQPGSYTQVSTDSFVFQYYYQHGYAPISPLPAVALRTVGWDADTGYQHI
                     FIPTTGTDKLSHRDSNNGNYFWEHWSRQTDSANLQVNHYRRVLYLWNYISGPHTVNYC
                     NS"
     gene            843..1346
                     /locus_tag="PKH10_02880"
     CDS             843..1346
                     /locus_tag="PKH10_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27100.1"
                     /translation="MIIGFLLLLTIFGVSGFFLIQQNERLSEIEASVLRIEGRVSDVE
                     RNYDKVHQEGVPKILSRRDKEVDSLISSLVSRVEVIETRLTAMESKEEEEETKRKDIP
                     IEEVQHVTTFVEEKQDSYFSDILEKCFKKKKGEVLDPKEEIEYQMLWGLLNEEKRTEF
                     NAACQDN"
     gene            1372..2262
                     /locus_tag="PKH10_02885"
     CDS             1372..2262
                     /locus_tag="PKH10_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27101.1"
                     /translation="MGLLIALFLFCDFNENTRESEEAEKIKIIFYTTVSDKASDRVEY
                     NLFDVLMSFEPYVEYSIYFAAPLGKDDSRELQKEICLYNKNKELFFEYSKCANKANEK
                     HLNFRCDDIYLSGANSNEERDSLMSCIDDETEWKVLSSDQEKMLEFNVEKRFPLLLIN
                     NRPYSGGLRKDDYKKGFCEQMEPAPEPCRFVNEPKEQKLFVITDRRCQKCEGLSFAIT
                     SMLKEYYENMKVTTLYYQNDDGKKLYDEISQHISLLPAFCFSTPIDEDEMFFWMQDHF
                     VRVGKNYCLKDSLPQYDPRR"
     gene            2573..5686
                     /locus_tag="PKH10_02890"
     CDS             2573..5686
                     /locus_tag="PKH10_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HNT27102.1"
                     /translation="MKVKMVLIVLVVLFSFILHAEQGVMLGGTPYKGWEEGGNDFVVM
                     FNSLIDDQITLTSETSSENRQGDTCILESSFDLTGLHIPDDAELIEAYIIWMGAVDPT
                     KFNDPTDNQVHLKFVRDDAGYTWEDDIIVGDVAKAVGQDDQNDLFAFQSVKFSPKDGV
                     TTGCSPDSPGTNTTGEVAYFTYRKDITDFFAKIRDDNLASENPRKDGLAMFGKYTVSG
                     LDCTDHDYYKCRTTMVSTWSIILLYKSGSVRSKKIYLYPGFAWAQGETTTANVSGFIL
                     PKNPVLRITTNIAEGDPALTPKLDLPPEIVYIKGPDAMSTYQLTNECNPQVDMSFEIY
                     NSRSSIIGWKGDLMCVSGVAGGPDYFGIDNDTFLLDAENDINLQEHLKLGGTNFDVRI
                     SVNQDAILMNYLVVSVDTKSPAFDIPPEAQINFPEDREKHYCSCRKAEDPADAYCVTE
                     SGGYPMYYLVRVQNWGTNVAKNVIVVDDLSNLVEYIPGSTEIATKFEDTNNPQNGTDW
                     TVIPDLAGNGTDPNTKFPLSGAGYKVADSMDICNQATWTCTDTRLLRFKVKPKKLPKV
                     EVIPNIAVIKEEGSVDAYRSNRGLNLKLYRGTCQPLETCPEPKKEECGGVALEERECD
                     ENKPCPDGSICNMTTFTCGPDPNKICTSAKVSYALGINSPDSGDSPIIVPKGTTGLTL
                     GQFKLQAENCGQTKYYGFVSVRMEIKKDDAQVAVTNLSLVHDKDGNGVADPDDPVLIN
                     VPTPEATSVLFAIPEGTARLFTGTEVHHFIVTGDLGYNITKVPAAASVHLTIANKNSF
                     LFEDAGDARPDGGTIDFASFQVEPTDNYFIVTKGMNDPSVPPREELNRDIPMLQVRTK
                     ALDLANKIKRITVTVPTQEGYARFGQGIKDISLYVDTNGDGISDDLIQKISTFEEDDE
                     VTFEGDDIETRLDYDVGEEMFLIFQCTLNIKLDGTERAKIVIRKGAVIMDNTSKKVEG
                     LAVSSKEFYPSDDVQPTDGDIVPPASSSCDCSLIAGEGDEPPAWLVLLAAALAALFPF
                     LRRRTTA"
     gene            5754..>8698
                     /locus_tag="PKH10_02895"
     CDS             5754..>8698
                     /locus_tag="PKH10_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27103.1"
                     /translation="MSGFRTAFFVLFILLIAGAAGAKEIGPNVAVFKGQDLKWEDGAY
                     SYHVMFKSLLANLNTGDDGTGVNYNPQADACLDQSTYTLDMTKFPLDATIDRAFIVWT
                     GAQPTLKLTDFTDNQIRLDFAHNDNPAMNSGLDVIGRQSKLTDKQDFEFESIIENETV
                     ERGFFTYRVEVTDFFKELQERGRSLEISDGSVDGLSLYGDYTVSGLECTADTAYKDNS
                     TMVSSWAIILVFVSEEVTPKKLYLYDGFYRYFHKNSIIDITGFQFPDKPSIRLTLMVN
                     EGDPRLVNTNTASGGSLVPESLRIRGAGAGYEQLFNTCNFLDQSILYGTRNTPFYYTE
                     IFNSISSEFGFDATSGETCIGGTPPNILTDQMEYAIDVDTFYIDAAEEKWAAHFYKNG
                     DQISLDLGANQDAVLTNFLILSHDTRAGNYDIPNEDEKGFCSCAPEEGKVCFTQEFTY
                     LIKVQNWGDDIVGGVKVQDTLPAYVDYVPGTTEVASQITEDAKGNWLGADWTPIPDGP
                     GGEFPLKQPYTVADQMNYCELPAEECPGTHDRRLVRFKVKPKDNLPKTSVIDNVAVIT
                     DAAMKPYKTNTSIPLKLKSGTCLPSAECEAMDMNKCGGVSDYTPPDDGDVVPDADTGG
                     GPCTTKNVAIAYAMGKNSPFGDVVIPNPSNNLTLGQFTMVGTTASEESACTFNLVKVK
                     VKVDRDTAVTLQNLKLVEDANQNGRYDNGENVVAETANLASGYADFDVPSTKRAFKEN
                     KTHYFVIHGDASVAEGTTITPNTTFKAIIEGATSIDVGDDGTPTVANKRIDFALYMFE
                     PTGKSFIFSRGPKDPAVPATLAEINANIPVLQLRTKSKEEANSIKSLTFKVPSGSVLF
                     GEGLEKVSVWLDNGGDGTADTLLGEGSAEAGESEYKLTFTTPIAYTADQEKYLVIKAD
                     FNLSGDQKGSIQVKKAPGTLDSEVTIYSLPIDSKVFQACTDPNDPNCQTGGDEEEGGL
                     GRILEGVVRA"
BASE COUNT         2175 a         2374 c         2287 g         1862 t
ORIGIN      
        1 cgtcccgata gcgttccttt tcatcatcgg cgccgcgttc ctcctgcaag ttaaagaata
       61 aagaaatcag caggttgatt tgacagctaa ttgatcaaga tagctttttg cataaaatat
      121 cggatgattg cattctatat tatggtaaaa atatattgag aatactttat ttgcgttttt
      181 cctatggtac ctatgttact gcgggttcgg atttttttta agggaggttc ctcatgaaaa
      241 agattgttgt cctatttttt ttcttgacgc tttcaatttc ctgttatgcg actagcttgg
      301 cagacacatt gtcgagaggg tacgggactc ctgtcaccta ttggtattgg tacgcttggc
      361 agggagaaga cggcaatttc tacaatggtg ggtgtcaacc cggttcttac acacaagtat
      421 caacagattc cttcgttttc cagtattatt atcagcacgg ctatgccccg atatccccac
      481 tgcccgcagt tgctcttaga accgtaggtt gggatgccga taccggatac cagcacattt
      541 tcattccaac aacggggaca gataaactgt cgcatagaga cagcaacaac ggcaactatt
      601 tttgggagca ctggtctcgg cagacggaca gcgccaattt acaggtcaat cattatcgcc
      661 gtgttctcta tctttggaat tacatcagcg gtccccacac ggtgaattat tgtaactcgt
      721 aaacccgatt atttgttgca atgttttttt ttccggaccc gcaagagttt ttgttatgaa
      781 gaaggataag agacatttgt tttgcaaatc ttactcgtga aagaatgaat cgtttttcgt
      841 gtgtgatcat cggttttctt cttttattga caatttttgg tgtgtcaggt ttttttctca
      901 tacagcaaaa tgaaagattg agcgagatag aggcatccgt attacgcata gaaggaagag
      961 tgtctgatgt agaacgaaat tacgataagg ttcaccaaga aggtgtcccc aagattttgt
     1021 caaggagaga taaagaggtt gattccctga tatcctcgct cgtgagccga gtagaagtaa
     1081 tcgagacgag gctcactgcc atggagagca aagaagaaga ggaggaaacg aaaagaaaag
     1141 atatccctat cgaagaagtg caacatgtaa cgacttttgt tgaagaaaaa caagactctt
     1201 atttttctga tattttggaa aaatgtttta agaagaagaa gggagaagtt ctcgatccca
     1261 aagaagaaat agaatatcaa atgctctggg gattgttgaa tgaagaaaag cggacagaat
     1321 ttaatgcggc atgccaagac aactagggtc cttttaacga taatggccct tgtgggacta
     1381 ttgatcgcgc tgtttctttt ctgcgatttt aatgagaata cacgggaaag cgaagaagca
     1441 gaaaaaataa aaatcatttt ctataccaca gtttccgata aggcaagcga tagagtggaa
     1501 tataacttat ttgatgtgct aatgagtttt gagccgtatg tagaatactc gatatacttc
     1561 gctgctcctt tgggcaagga tgactcccga gaattgcaaa aagaaatttg cttgtacaat
     1621 aaaaacaaag aacttttttt cgaatattca aagtgtgcga ataaggcgaa tgaaaaacat
     1681 cttaatttta gatgtgatga catatacttg tcgggtgcca acagcaatga ggagagggat
     1741 tccctcatgt cttgtattga cgatgaaact gaatggaagg ttctttcgtc tgaccaagag
     1801 aaaatgctag aattcaatgt cgaaaaaaga ttcccgttgt tattgattaa caacaggccc
     1861 tatagtggtg gtctgcgaaa agatgattac aagaaaggat tttgcgaaca aatggagcct
     1921 gctccagagc cgtgccgttt tgtgaacgag ccaaaagaac aaaaactctt tgtgataacc
     1981 gatcgaagat gccaaaaatg cgaaggatta tcttttgcaa taactagtat gttgaaagag
     2041 tattacgaga acatgaaagt aaccacgctc tattatcaaa acgatgatgg caagaaatta
     2101 tatgacgaga tatctcaaca tattagcttg ttgccggcgt tttgtttctc aacaccgatt
     2161 gacgaagacg agatgttttt ttggatgcaa gatcactttg tgcgggtcgg gaagaattac
     2221 tgtctcaaag actcgttgcc gcaatatgat cctcgaagat aaggggaagg ttcccgcaat
     2281 tgtggggctg aacttttcat tgtcgggaca agagggcatg tccttgcttt ttcgtccttg
     2341 accttgacga ccttatcttt gctcaacttc atcgccgata ttttttctgt tccctcttga
     2401 ttttctcctc agttgcagtt taatatgagg ctgttggttt ggcatacggt tttcaaagag
     2461 ttgtttgtta atggcgactg acgacggaat gggaaagatt ggccatcaat ggagtttcgc
     2521 aagttgtttc ttatacgatc gatcttgcaa catatcgttt agggagaaac tgatgaaagt
     2581 taagatggtt ctcatcgtgc tcgtggttct attttccttc atccttcatg ccgagcaagg
     2641 tgttatgctc ggcggaacgc cctacaaagg gtgggaagag ggaggcaacg atttcgtcgt
     2701 catgttcaat tcgcttatcg atgatcaaat caccctgacc agtgaaacaa gctccgagaa
     2761 ccgacagggc gacacatgta ttctcgaaag ttcgttcgat ctgaccgggc tccacattcc
     2821 tgatgacgcc gaattgatcg aggcttacat catttggatg ggggcggtcg atccgaccaa
     2881 attcaacgat ccgaccgaca atcaggtcca cttgaaattc gtacgggacg atgccggcta
     2941 cacttgggag gatgacatta tcgtcggtga cgtggccaag gcggtggggc aagacgacca
     3001 gaatgacctt ttcgcattcc agagtgtcaa attttccccc aaggacggcg tgacgaccgg
     3061 ctgttccccc gatagccccg gaacgaacac caccggtgag gtcgcctatt ttacctaccg
     3121 caaggacatc accgacttct tcgcaaagat acgtgacgac aaccttgcgt cggaaaaccc
     3181 gcgcaaagac ggtcttgcta tgttcggcaa gtacaccgtg agcggccttg actgcaccga
     3241 ccacgattat tacaaatgcc gcaccacgat ggtctccacc tggtccatca tccttctgta
     3301 caaatcgggg agcgtccgtt ccaaaaagat atacctctac cccggcttcg cgtgggcgca
     3361 gggtgaaaca acgaccgcca atgtttcagg ctttatcctt cccaagaacc cggtgcttcg
     3421 tatcaccacc aacatcgccg agggcgatcc ggcgttgacg cctaaactgg acctgcctcc
     3481 cgagatcgtc tacatcaagg gccctgatgc gatgagcacc taccaactca ccaatgagtg
     3541 caatccgcag gtggacatga gtttcgagat atataactcc cggtcctcaa tcatcggctg
     3601 gaagggggat ctcatgtgcg tcagcggcgt ggcgggcggc cccgactact tcggcataga
     3661 caacgacacc ttcctgctcg acgccgaaaa cgatatcaac ctgcaggaac acctgaaact
     3721 gggcggcacc aacttcgatg ttcggatatc ggtcaatcag gacgccatcc tgatgaacta
     3781 cctcgtcgtg tcggtcgata cgaagtcgcc cgcgttcgat attccgcccg aggcgcagat
     3841 aaactttccc gaagaccgtg aaaaacatta ttgttcctgc cgcaaggccg aagacccggc
     3901 tgatgcctac tgcgtcaccg aatcgggcgg ctacccgatg tactacctcg ttcgcgtcca
     3961 gaactggggg accaatgtgg ccaagaatgt catcgtggtg gacgatctta gcaatctagt
     4021 ggagtacatc cccgggtcga ccgagatagc gaccaagttc gaggacacga acaaccccca
     4081 gaacggcacc gattggaccg tgatacccga tcttgccggc aacggcaccg atcccaacac
     4141 caagttcccg ctgtcgggcg ccggctacaa ggtcgccgac tccatggata tctgcaatca
     4201 ggcgacttgg acctgtaccg atacgcgact tctccgcttc aaggtgaaac cgaaaaaact
     4261 gcccaaggtc gaggtcatcc cgaatatcgc ggtcatcaag gaggagggga gcgtcgatgc
     4321 ctaccgcagt aaccgaggcc tgaatctgaa actttaccgc ggcacctgcc agccgctcga
     4381 gacctgcccc gaaccgaaaa aggaggagtg cggcggcgtg gcgcttgaag agcgcgagtg
     4441 tgacgagaac aagccctgcc ccgacggttc gatctgcaac atgaccacct tcacctgcgg
     4501 tcctgacccc aacaagatct gcaccagcgc caaggtctcc tacgcgctcg gcatcaattc
     4561 ccccgacagc ggcgacagcc ccatcatcgt acccaaggga acgaccggcc tgacgctggg
     4621 ccagttcaaa ctgcaggccg agaactgcgg ccagacgaaa tattacgggt tcgtctcggt
     4681 gcgcatggag ataaaaaagg acgatgcgca ggtggcggtg accaatctct cgttggtaca
     4741 cgacaaggat ggtaacggcg tcgccgatcc cgacgacccg gtgctcatca atgtgcccac
     4801 tcccgaggcc accagcgtcc tgttcgcgat acccgaagga acggcccgtc tgttcaccgg
     4861 caccgaggtg caccacttca tcgtgacggg agatctcggc tataacatca ccaaggtccc
     4921 ggccgccgct tcggtacatc tcaccatagc gaacaagaat agtttcctgt tcgaggacgc
     4981 cggcgacgcg aggcccgacg gcggcacgat agatttcgca tcgttccagg tcgagccgac
     5041 cgacaactac ttcatcgtca ccaaggggat gaacgacccg tcggtgccgc cgcgtgaaga
     5101 gctcaaccgc gacatcccga tgcttcaggt ccgcaccaag gcgctcgatc ttgccaacaa
     5161 gataaagcgc atcacggtga ccgttcccac gcaggagggg tacgcccggt tcggccaggg
     5221 catcaaggat atatcgctgt atgtcgatac caacggtgac ggcatctccg acgacctcat
     5281 ccagaagata tcgaccttcg aggaggacga cgaggtcacc ttcgagggtg acgacataga
     5341 gacccggctc gactacgatg tcggcgagga gatgttcctc atattccagt gcacgctcaa
     5401 catcaaactt gacgggaccg agcgggccaa gatagtcatc aggaagggtg cggtcatcat
     5461 ggataacacc agcaagaagg tcgaaggtct cgcggtgtca tcgaaggaat tctacccctc
     5521 cgacgatgtc caaccgaccg acggcgacat cgtgccgccc gcttcctcgt cctgcgactg
     5581 ctcgctcatc gccggagagg gcgatgaacc gccggcgtgg ctcgtcctgc tcgcggcggc
     5641 tctcgcggcc ctgttcccgt tcctgcgtcg ccgcacgacg gcctaggcgg gaccggcatg
     5701 cttttttcga tgatggacca tactacccaa catagccaaa aaagaggtgt cacatgagcg
     5761 gctttcgtac ggccttcttc gttcttttca tcctgctgat cgccggcgcg gcgggggcca
     5821 aggagatcgg tcccaatgtc gccgtgttca aggggcagga cctcaagtgg gaggacggcg
     5881 cctacagtta ccatgtcatg ttcaaatcgc tactcgccaa cctgaacacc ggggacgacg
     5941 ggaccggggt gaattacaac ccgcaggccg atgcgtgcct cgaccagagc acctacacgc
     6001 tcgacatgac caaattcccg ctggacgcga cgatagatcg cgccttcatc gtctggaccg
     6061 gtgcccagcc gacgctcaaa ctcaccgact tcactgacaa ccagatacgg ctcgatttcg
     6121 cgcacaacga taacccggcg atgaacagcg ggctcgatgt gatcggtcgc cagtcgaaac
     6181 tgaccgacaa gcaggatttc gagttcgaat ccatcatcga gaacgagacc gtcgaacgcg
     6241 gctttttcac ctaccgcgtc gaggtgaccg acttcttcaa ggaactgcag gagcgcggtc
     6301 gttcgctcga gatatcggac ggctcggtcg acggcctgtc gctctacggc gattacaccg
     6361 tttcgggcct tgaatgtacc gccgacacgg cctacaagga caattcgacc atggtatcga
     6421 gttgggccat catcctcgtg ttcgtttccg aggaggtcac cccgaagaaa ctctacctgt
     6481 acgacggctt ctaccgctat ttccacaaga attccatcat cgacatcacc gggttccagt
     6541 tccccgacaa gccctccatc cggcttaccc tgatggtcaa cgagggcgac ccgcggctcg
     6601 tgaacaccaa taccgcgtcg ggcggcagtc tcgtccccga gtcgctccgg atacgcggcg
     6661 ccggcgccgg ctacgagcaa ctgttcaaca cctgcaactt cctcgaccag tcgatacttt
     6721 acgggacgcg caatacgccg ttctattaca ccgagatctt caactcgata tcctctgagt
     6781 tcgggttcga cgccacctcg ggcgagacct gcatcggcgg aacgcccccc aacatcctga
     6841 ccgaccagat ggaatacgcc atagatgtcg acaccttcta catcgacgcc gccgaagaga
     6901 aatgggccgc ccatttctac aagaacggcg accagatatc gctcgatctc ggcgccaatc
     6961 aggatgcggt gctcaccaac ttcctgatac tgagccacga tacccgcgcc ggcaactacg
     7021 acatcccgaa cgaggacgag aaggggttct gctcctgtgc accggaagag ggcaaggtct
     7081 gcttcacgca ggaattcact tacctcatca aggtgcagaa ctggggcgat gatatcgtcg
     7141 gcggcgtcaa ggtgcaggac accctgcctg cctacgtcga ctatgtcccc ggcaccaccg
     7201 aggtcgcgtc ccagataacc gaggacgcga aaggcaactg gctcggcgcc gactggacgc
     7261 cgatacccga cggtcccggc ggcgagttcc cgctcaaaca gccctacacg gtcgcggatc
     7321 agatgaacta ctgcgaactg ccggccgagg agtgccccgg tacgcatgac cggcggctcg
     7381 tccgcttcaa ggtgaaaccg aaggacaacc tgcccaagac ctcggtcatc gacaatgtcg
     7441 cggtcatcac cgacgcggcg atgaaaccct acaagaccaa cacctcgata ccgctcaaac
     7501 tcaagagcgg cacctgtctc ccgtcggccg aatgcgaggc gatggacatg aataagtgcg
     7561 gcggcgtcag cgattacacg cctccggatg atggcgatgt cgttcccgac gccgatacgg
     7621 gcggcggccc ctgcacgacc aagaatgtcg ccatagcgta cgcgatgggc aagaacagcc
     7681 cgttcggcga tgtggtcatc cccaatccgt cgaacaacct gacgctcggc cagttcacca
     7741 tggtggggac caccgccagc gaggagagcg cctgcacctt caacctggtc aaggtcaagg
     7801 tcaaggtcga tcgtgacacg gcggtgaccc tgcagaatct caagctggtg gaggacgcca
     7861 accagaacgg ccgctatgac aacggcgaga atgtggtggc cgagaccgcg aaccttgcgt
     7921 cggggtacgc agatttcgat gtcccctcga ccaagagggc gttcaaggag aacaagaccc
     7981 actatttcgt catccacggc gacgcgagcg tggccgaagg gacgacgata accccgaaca
     8041 ccaccttcaa ggcgatcatc gaaggggcca cctccatcga tgtgggcgac gatggcacgc
     8101 cgacggtcgc gaacaaacgg atagatttcg ccctctatat gttcgagccg accggtaagt
     8161 ccttcatctt ctcccgcggc ccgaaggatc cggcggtgcc ggcgacgctg gccgagataa
     8221 acgccaacat cccggtcctg caactgcgca ccaagtcgaa ggaagaggcg aactcgataa
     8281 aatcgctcac cttcaaggtc ccgagcggga gcgtgctgtt cggcgagggg ctcgaaaagg
     8341 tgtcggtctg gctcgacaac ggcggcgacg gcaccgccga taccctgctc ggcgaaggaa
     8401 gcgccgaggc gggtgagagc gagtacaaac tgaccttcac aacgccgata gcctacaccg
     8461 ccgatcagga gaaatacctc gtcatcaagg ccgacttcaa cctatcgggc gaccagaagg
     8521 ggagcataca ggtcaagaag gcgcccggta cgctcgacag cgaggtcacc atctactcct
     8581 tgccgataga ttcgaaggtc ttccaggcct gcaccgaccc gaacgacccg aattgccaga
     8641 cgggcggtga cgaggaagag ggcggccttg gtcgtattct ggagggagta gtccgtgc
//
LOCUS       DAOQUE010000057         8572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_9151_length_8572_cov_4.521663, whole genome shotgun sequence.
ACCESSION   DAOQUE010000057 DAOQUE010000000
VERSION     DAOQUE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9151_length_8572_cov_4.521663"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKH10_02900"
     CDS             <1..336
                     /locus_tag="PKH10_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27104.1"
                     /translation="INREMGPFVLAMKNARDLARSGKEVVLVFEDKATALLFELKDPS
                     RLSTMLFNDLIKARMVIICRTCAMKTNAFFTLDQQKLDKIQVVGEPEMDGHVPMREYI
                     DRGFQIIVV"
     gene            498..2024
                     /locus_tag="PKH10_02905"
     CDS             498..2024
                     /locus_tag="PKH10_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT27105.1"
                     /translation="MKSNGTADCLCVRTGMYEKAFTVARPVKIFGEKGKESVLTQTGG
                     VTVTVAADEVTLKDLVIRAEAPGSYAVSAEGTETQKKEGLTIDSCQIEANDNSHGIKA
                     ANTTGIIIQNGIIIQNGIIEEESIKPIGVHLTDSTDISITGTEFQNNGGVHEDKASEF
                     NKSGASGSWSLVVQGGAEKSEISLKDSSFTDGNKEALLTTALRAENSDITVESAAVSG
                     FTMAYAFIDSTATVKGGTVKDIKATIAAMYASNTRLTLQKGTYMAITNVDKNATIDTS
                     GGGRGLALVNGSVLSATGIDVSDCEAQGILFENSSGTLNDLRINDNSQVGIWIQETAD
                     KKISGKKIVIEDSELEGNGGAGIFVVAKPEEVEDLSGIEIRNTAISATKLVQGKSVMA
                     DGLVVRSLSLHSEELDVVVLDNVTFENNTRTGAIFDGIASSTTPLTGIKTSEVKVKED
                     TTGENTENKYGIIIQNGKPPDGFQAGITENPFKQKDLNFMDAGSPLKIGTETVAIPAL
                     "
     gene            2057..3280
                     /locus_tag="PKH10_02910"
     CDS             2057..3280
                     /locus_tag="PKH10_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27106.1"
                     /translation="MRSYLWLILGLTTMLMIACDDGGMGDPNETDTGGDTGDTGDTGD
                     TGNDEDAGGELVEVIWSDQIGSKESDRASDIVFNNGEICVTGDAGGDIDGFNGNKGSS
                     DILFSCWDSAGYLIHTSMLGTDKWDNGSSIVSDEFGNYYITGKTAGSLDEDEYINTGL
                     GQYDAFLMKLQGGYPYPEWTKQWGTDGMDAVGALLIDSQGYVYVCGDMDEYQDQCFLI
                     KFSSYEEEVWKKNWGGDPFCTCNSVAADSNHNLYATGSMSDSGVFLTKITADGQISWL
                     KKFGPTVGENAYKVKIDSNDKIFVVGSSGLTKFDTDGTPLWTAENNGGDAIAFDDEGN
                     IYVVGSNESFLTKVAGDGNEVWRWEWGTGYTHGNAITIYSNDVYVAGTTMGSLPDNES
                     SGSYDVFIVKVRLLE"
     gene            3360..6170
                     /locus_tag="PKH10_02915"
     CDS             3360..6170
                     /locus_tag="PKH10_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT27107.1"
                     /translation="MKRIFSKLFLPFLLLIACAETSTDPLDNEATADADTYDFWKDGP
                     QRPYPVDWGTCSEGWEPKEEKDEEGNLLAKYCEPPTNWRPVAPVDWGDCPEGWEPMEE
                     KDEDGNLLAKYCEPTLCPDGEFAVIPAETGTEIIYVYEGESIQAAIDGAQDGAVIAIG
                     KGTFTEEISIMYSPSKAVTLWGACVEGTTIRSETPTTATMAAVISIRQANKEKLVTIK
                     NLTVTGRRIGVFVGSFSQVRLEQMAIEESTEMGLFAESEGKPTVNIDSVLIRGTLPRE
                     SDGMLGRGVYIQGNVTATLSRVLVEKNKEAGVAVGAFEGTHATFSATDLIVRDTLPQK
                     DNSGGEGIGIQVGNQDKVTVSFSRLLVERNIGAGIYASAPSPDSHIAIEKADDLIVRH
                     TRPDARDQTMGRGIEIEDNVTAFFSRVLIEKNKEVGVFILTTNSESFSTVTAANLVVR
                     DTEPQQVDQTAGRGIAVQGNVTATLSNTLVEKNTDVGFSVRVPLVATHAKITATNLIV
                     RDTFPQNDGTNGRGIIIEDNVTATFSHMLVERNSDAGVFMASFVSGTSPEITANDFIV
                     RDTLLQGSDQTGGTGIAIQKTGTATLVRGLVERNAGTGIYVSASSPESHITLKESTDL
                     LVRHTGDATENAGDLTFGRGIAIQDNVTATLSRTLVEKNREVGFFVSTATAGTHASVN
                     ATDLIVRDTLPQNDKTAGRGLVIQSMVSATFFRLLAEKNRDVGFFVSASTVGTHASVN
                     ATDIIVRDTESQESDKTGGRGINIQFAVDATMTNIQVENNREMGLLVAGQEASAQLTH
                     AEITDTRILECYGVENGCEEPFGHGLGVYEGSEVTLTDVVIDGNQNGLQMTNSKVFAG
                     ENCTLPLGDKEGKVACLHLLNNQTGLNAFGEPIEGYDRDEAFALERNGGTWYSGNETP
                     FSSAEEAIPQPMESTIDSGTKE"
     gene            6186..>8572
                     /locus_tag="PKH10_02920"
     CDS             6186..>8572
                     /locus_tag="PKH10_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27108.1"
                     /translation="MKRILFPVFFSLLLFGACAEKKETPTDAEPTDSEQPDSDDFFVV
                     GPKPPDWGTCTGGWEQKEQVNEETDRLEARWCEPPANWRPVAPVDWGTCPEGWEQKED
                     KDEDGNLLARYCDPVLPVECGAGEMMVLGFAECQPVGEDCPASDFADIPVAAGSPVFE
                     VAAGGSIQDAIDGAPDGAVISIGKGTFDEFVTIVDKNITLWGACVTETILASTTPMED
                     ETTDATVKIEGTDPEKKVVVRNLTITGENRRGIIIQNGITGDFNNLVLTKAKRAGITV
                     KNSTADLTRVLVQETLPGTSGRGHGVSIAGSLTTTLSQVSLMDNDGFGMGFSTDTEQK
                     GTLVATDVLVSGSGDTGVGLYDNVSATLTDILVRNSEFWGLSAVTFGENEQLSLNATR
                     IIIRDTNNGYGMALQNQENQIVGKVEANLEQVLIERNRLVGLSALTLAGASVEIKKMD
                     DIIVRDTLSDSTTQDGGIGVFLMGDVSVKEEGGHRILIDNNIEQGLLAYTKGLETTMF
                     LVLNDLAVRGTRLSESTWQFGQGIFIANHVVAAFSGVLLENNRTVGLHIATYEEDIEP
                     GSSCEVNVSDMLVRTTLVEEVDQTGGYGVGVNGNVEVLFERALIEENRSAGIVVVTET
                     AAANARVTGNSIIVRGTQPQKADELGGAGMSFANNVDVVMSKALVNENSAAGILTDTI
                     VAGARVNFRAEDIIVRDTLARQEGTFGRGMNFQNNTEAAIIRAFVDNNREVGIFAASA
                     DANRTTLFAGTDLMVSDTRARASDKQKGNGIIIQNGARAIITDTAVVRNREVGLM"
BASE COUNT         2260 a         2006 c         2440 g         1866 t
ORIGIN      
        1 atcaaccgag aaatgggacc cttcgtcctc gccatgaaga atgcgcggga cctcgcccga
       61 tcgggtaaag aggtcgtgtt ggtgttcgag gacaaggcga ccgctctgtt gttcgaactc
      121 aaggacccgt cgcggctctc gacgatgctc ttcaacgacc ttatcaaggc gcgtatggtc
      181 atcatctgtc gcacctgcgc gatgaagacc aatgccttct tcaccctcga ccagcagaaa
      241 ctggacaaga tacaggtggt cggcgagccg gagatggatg gtcatgtccc gatgagggag
      301 tatatagacc gcgggtttca gataatcgtc gtgtaaaaga tagaggtgct gctatgaagc
      361 gtttttcctt tttggtctcg gtcgttaccg cttgcgctgg gttttggctt ttgtcgtgcg
      421 ataaggcacc ttgcgacatg cccgctacct gcaaaaagtc tgtcgatgtc ttctctgtcg
      481 ccgaccttga aaaagagttg aaaagtaacg gcacggccga ctgtctctgt gttcgtacgg
      541 gaatgtacga aaaagcattc accgtcgccc ggccggtgaa aatattcggg gagaagggga
      601 aggaaagcgt tctgacccag acagggggtg ttaccgtcac ggtggcggcc gacgaggtga
      661 cgctgaaaga ccttgttatc cgggccgaag ctcccggaag ttatgccgtg tcggcggaag
      721 gcaccgagac gcagaagaaa gagggcctga cgatagattc ctgccagata gaggcgaacg
      781 acaacagcca cggcatcaag gcggccaaca ccaccggcat catcatccag aacggcatca
      841 tcattcagaa cggcatcatc gaagaggaga gcatcaaacc gataggcgtt cacctgaccg
      901 actcgaccga tatctccatc acggggacag agttccagaa caacggcggc gttcacgagg
      961 acaaggcctc cgaattcaac aagtcaggag cgtcgggcag ttggtccctt gttgtccagg
     1021 gcggcgcgga aaagagcgag atatctctga aggactcctc tttcaccgac ggcaacaaag
     1081 aagcattact caccaccgct ctgcgcgccg aaaatagcga catcacggtc gagagcgccg
     1141 ctgtttccgg ctttaccatg gcctatgcgt tcattgattc gaccgccacc gtaaaaggtg
     1201 gcacggtgaa ggacatcaaa gccaccatcg ccgccatgta cgctagcaac acccggttga
     1261 cgcttcaaaa agggacctat atggcgataa cgaatgtgga caagaatgct acgatagata
     1321 catccggcgg cggtcgcggt ctcgcactgg tgaatggttc agtgctttcg gctaccggca
     1381 tcgatgtctc tgactgtgag gcacagggaa tcctttttga gaactcttcg gggacgctga
     1441 atgacctgcg cataaatgat aacagtcagg tcggtatctg gatacaggaa accgcagaca
     1501 agaaaatcag cggaaaaaag atcgtcatcg aggactcgga actcgaaggg aatggtggtg
     1561 ccggcatttt cgtcgttgcg aagccggaag aagttgagga tctatcgggg atcgaaataa
     1621 ggaacaccgc catttctgcg accaaattgg tacagggaaa gagcgtaatg gctgacggcc
     1681 ttgtcgtgcg tagcctttcg cttcattcag aagaattaga tgtggttgtg ctcgacaatg
     1741 tgactttcga gaacaacacc cgcaccgggg cgatattcga cggcatcgca tcgtcaacga
     1801 cgcctttgac aggtataaag acgagcgaag tcaaggtaaa ggaggatacg actggagaga
     1861 acacggagaa caagtacggc atcatcatac agaatggaaa accccctgac ggattccaag
     1921 cagggataac cgagaacccg ttcaaacaaa aagatctcaa cttcatggat gccggttcgc
     1981 ccctcaaaat cggtacagaa accgtcgcga tacctgccct atgacgattt tataaatata
     2041 acactggaaa tctgccatga gaagttatct ttggttgatt cttggtttga caactatgct
     2101 gatgatcgcc tgtgacgatg gtggaatggg agatccgaat gagactgata caggagggga
     2161 cacaggagac acaggagata cgggagatac ggggaatgat gaagatgcgg gaggcgaatt
     2221 agttgaagta atatggtcag atcagatcgg atcaaaggaa tctgatcggg cctccgacat
     2281 tgtttttaat aacggagaga tatgtgttac cggtgacgcc gggggcgata tagatggttt
     2341 taatggaaac aaggggtcca gtgacatcct tttttcctgt tgggacagcg cgggatatct
     2401 gatacatacg agcatgttgg gcaccgacaa atgggataac ggaagctcaa tcgtgagcga
     2461 cgaattcggt aactattaca ttaccggtaa gactgccggg tcactcgatg aagatgaata
     2521 catcaatacg ggactaggac aatacgatgc tttcctaatg aaattacaag gcggatatcc
     2581 ctatcctgaa tggacaaaac aatggggaac agatggaatg gatgcggtgg gggctctttt
     2641 aattgactcg cagggatatg tgtatgtttg tggcgatatg gacgaatacc aggaccagtg
     2701 ttttttgata aaattcagtt cttatgagga agaagtctgg aaaaaaaatt ggggcggaga
     2761 tccgttttgc acctgcaact cagttgccgc cgacagcaat cataatttat acgctacggg
     2821 aagtatgagt gatagcggtg tgttcctaac aaaaataact gcggatggcc aaatatcatg
     2881 gttaaagaag tttggaccga ctgtgggtga gaatgcttat aaggttaaaa ttgacagtaa
     2941 cgataagatt tttgtggtgg gttccagtgg attaacaaaa tttgacaccg atgggacccc
     3001 gctttggacc gccgaaaaca atggcggcga tgcaatcgct tttgatgatg aggggaacat
     3061 ctatgtggtt ggttcaaacg agtccttctt aacaaaagtt gctggtgacg ggaacgaggt
     3121 atggagatgg gaatggggaa cgggatacac acatggaaat gcaataacga tttattcaaa
     3181 tgatgtgtat gttgcaggaa ctacaatggg gagtttacct gacaatgaaa gttcaggatc
     3241 ttacgatgtt ttcattgtaa aagtgagact gcttgaatga tctttcctag acaacgcccc
     3301 cattttttca tcgtaatttt gttatttttt tttgggggag tgattaaaag gaggcagtca
     3361 tgaaaagaat cttttccaag ctttttcttc ctttccttct actgatcgcc tgtgccgaaa
     3421 cgagtacgga tccgttggat aatgaggcga ccgccgacgc cgacacctat gacttttgga
     3481 aggatggtcc gcaacggccg tacccggtgg actggggcac ctgctccgag ggatgggagc
     3541 cgaaagagga gaaggacgaa gaaggtaacc tcctcgccaa gtattgtgag cctccgacaa
     3601 actggcgccc cgtcgcaccg gtggactggg gcgattgtcc cgagggctgg gaaccgatgg
     3661 aagagaaaga cgaggatggt aacctcctcg ccaagtactg cgagccgaca ctttgccctg
     3721 atggcgaatt cgccgtcatc ccggccgaga ccggtaccga gatcatctat gtttacgaag
     3781 gcgagtccat ccaggcggcg atagacgggg cgcaggacgg tgcggtcatc gcgataggga
     3841 aagggacttt caccgaagag atatccatca tgtactcacc atcaaaggcg gtgacgctgt
     3901 ggggtgcctg cgtggaaggg acgacgatta gatcggaaac gccaacgacg gcaacaatgg
     3961 cggcggtcat ttcaatccgg caggcgaata aagagaagtt ggtgacgata aagaacctta
     4021 ccgtgaccgg tcgcaggata ggcgtgttcg tgggatcctt ttcgcaggtt aggcttgagc
     4081 aaatggccat cgaagaatcc acggaaatgg ggttgttcgc tgaaagtgaa ggcaaaccca
     4141 cggtgaacat tgatagtgtc ttaataagag gaacactacc tcgagaaagc gacggaatgc
     4201 tcggaagagg agtttatatc caaggaaatg tgaccgccac cctttctcgc gtgcttgtgg
     4261 aaaaaaacaa agaggctggt gttgcagtag gtgcttttga ggggacacat gccacttttt
     4321 ctgccactga tcttattgtg cgggacacat tgcctcaaaa agataactcg ggaggcgagg
     4381 gtatcggaat tcaagtcggc aatcaagaca aggtgacggt ctctttttcc cgtttgcttg
     4441 tcgaaagaaa cataggtgcg gggatttatg ccagtgcgcc ttctcctgac tcgcatattg
     4501 cgattgaaaa agctgacgac cttattgtcc gccatacgag gccggacgca agagaccaaa
     4561 caatgggcag aggtatcgaa attgaagaca atgtgaccgc ctttttttcc cgcgtactta
     4621 tcgaaaaaaa caaggaggtc ggcgtcttta ttcttaccac caattccgaa tcgttttcaa
     4681 cggttactgc cgccaacctt gttgtccgcg acacagagcc gcaacaagta gaccaaacag
     4741 cgggaagggg cattgctgtc caaggaaatg tcacagcaac cctttccaac acactcgtgg
     4801 agaaaaacac agatgtcggc ttttctgtcc gagtgcctct tgtcgctact catgctaaga
     4861 ttactgctac caatcttatt gttcgcgaca cttttcctca aaatgacgga acgaatggca
     4921 gaggcattat tatcgaagac aatgtgaccg ccactttttc ccacatgctc gttgaacgaa
     4981 acagcgatgc cggtgttttt atggcctctt ttgtttctgg aacaagcccc gaaattacgg
     5041 cgaatgattt tattgtccgc gatacattat tgcaaggaag cgatcaaacc ggaggaaccg
     5101 gtatcgccat ccaaaaaacg ggaacagcca cccttgttcg cggactagta gaaagaaacg
     5161 caggcaccgg gatctatgtc agtgcttcct ctcctgaatc tcacataacg ctcaaagagt
     5221 ctaccgacct tcttgtccgc catacaggag atgcaactga aaatgcagga gacttaacat
     5281 ttggcagagg catcgctatc caagacaatg tgaccgccac tctttctcgc acacttgtgg
     5341 agaaaaaccg agaagtcggc ttctttgtct ccaccgctac cgctgggaca catgcaagcg
     5401 ttaatgcaac cgaccttatc gtccgtgata ctctgcctca aaatgacaaa acagccggac
     5461 gagggcttgt catacaaagc atggtgagtg ccaccttctt ccgattactc gcggagaaaa
     5521 acagagatgt cggctttttt gtcagcgcct ctaccgtcgg aacacatgca agcgttaatg
     5581 caaccgatat tattgtccgt gatacggagt cacaggaaag cgataaaaca ggaggccggg
     5641 gcatcaatat ccagttcgca gtagatgcca ctatgaccaa tatacaagtt gagaataacc
     5701 gagaaatggg cctgcttgtt gccgggcaag aggcttccgc gcaacttact catgctgaaa
     5761 ttactgatac tcgtattctt gaatgttatg gggtcgagaa cggctgtgaa gagcccttcg
     5821 gtcacggtct tggcgtttat gaaggatcgg aagtgactct taccgatgtc gtgatcgacg
     5881 gtaaccagaa cggcctgcag atgacgaact ccaaagtatt cgccggcgag aactgcaccc
     5941 tgccccttgg cgataaggag ggaaaagtgg catgccttca cctgctgaac aaccagaccg
     6001 gcctgaacgc attcggggag cccatcgagg gatatgaccg tgacgaggct tttgctctgg
     6061 agagaaacgg cggaacttgg tacagcggta acgagacgcc tttctcgagt gcggaagaag
     6121 cgatccctca gccgatggag agcacgatag atagcgggac gaaagagtaa gcaatggaga
     6181 cgactatgaa aagaattctc ttcccggtct ttttttctct tcttcttttc ggcgcctgtg
     6241 ccgagaaaaa ggaaacaccg accgacgccg agccgacaga cagcgaacag ccggacagcg
     6301 acgatttctt tgtcgttggc cccaaaccgc ccgactgggg cacctgcact ggcggctggg
     6361 agcagaagga acaggtcaat gaagagaccg ataggcttga ggcgcgttgg tgcgaaccgc
     6421 cggcgaactg gcgtcccgtt gctccggtgg attggggcac ctgccctgaa ggatgggagc
     6481 agaaagaaga caaggacgag gatggaaatc ttctcgcccg atactgtgac ccggtactgc
     6541 cggtcgagtg cggagccggc gagatgatgg tgctcggctt tgccgagtgt cagcctgtcg
     6601 gggaggattg ccccgccagc gatttcgccg acattcccgt cgcggccggc tcgccggtct
     6661 tcgaggtggc ggcgggcggt tcgatacagg acgcgataga cggcgcgccg gacggtgcgg
     6721 tcatctctat cggcaaggga accttcgacg agtttgtcac catcgttgac aagaacatca
     6781 ccctctgggg cgcctgcgtc acggagacca ttcttgcctc tacaacgcca atggaggacg
     6841 aaacgaccga tgcaaccgtg aaaatagaag gcaccgatcc tgaaaagaag gttgtggtca
     6901 ggaacctgac catcaccggc gaaaaccggc gcggcatcat tattcagaac ggcatcaccg
     6961 gggacttcaa caacctggtg ctgacgaaag ccaagagggc agggataact gttaagaata
     7021 gtaccgccga tctcacccga gttctggttc aggaaacact gcccggaacg agtggccggg
     7081 gtcacggagt ttccattgcg ggttccttga ccactacgct ttcacaggtt tctttgatgg
     7141 acaatgatgg ctttggaatg gggttttcca cggacaccga acaaaagggg acgcttgtag
     7201 cgaccgatgt ccttgtttcc ggttcaggag atacgggggt cggattatac gataatgttt
     7261 cagcaacact taccgacata ttggtgagga acagcgaatt ttggggattg tcggcagtaa
     7321 cttttggaga gaatgaacag ttgtccttga acgcgacacg catcattata cgcgacacga
     7381 acaacggcta tgggatggcg ttgcaaaacc aagaaaacca aattgtgggc aaggtggaag
     7441 ccaacttgga gcaggtgctg atagaaagaa acaggctggt cggactctct gccctgaccc
     7501 ttgccggagc aagcgtggag ataaagaaga tggatgacat catcgtccgt gacacgctgt
     7561 cggacagtac tacgcaggat ggaggtatcg gtgtcttctt gatgggggat gtttctgtta
     7621 aggaagaggg cggtcatcgt atccttattg acaacaacat cgaacaggga ctgctcgcct
     7681 acacgaaggg gcttgaaacg acgatgtttt tggttctgaa tgaccttgcg gtgcgtggta
     7741 ctcggctaag tgaatcgaca tggcagttcg gtcaagggat ttttatcgcc aaccatgtgg
     7801 tagcggcttt ttccggggtc ctgctggaga ataaccgtac cgttggctta catattgcca
     7861 cttatgaaga agacatagaa ccagggagtt cttgtgaagt caatgtctct gatatgttgg
     7921 ttcgcacaac gctggtggag gaagtggatc aaacgggagg atacggtgtt ggcgtgaatg
     7981 gaaatgtgga ggtattattt gaaagggcat tgatcgaaga gaaccgttcc gcgggcattg
     8041 tcgtcgtaac ggagactgcg gcggctaacg cccgggtcac cggcaactcg atcattgttc
     8101 gcgggacaca accccagaaa gctgacgagc tggggggtgc cggcatgtcg ttcgcaaaca
     8161 atgttgatgt tgtcatgtcc aaggcgctgg ttaacgagaa ttccgctgcg gggattctca
     8221 ccgacaccat cgttgctggc gccagagtga actttagggc ggaagatatc atcgtgcgcg
     8281 atacgctggc gagacaagag ggaacatttg gccgaggtat gaatttccag aacaacacgg
     8341 aggcggccat catccgtgcc tttgtggaca acaaccgcga ggttgggata ttcgccgcca
     8401 gcgccgacgc gaacaggaca acgctctttg caggcaccga cctcatggtc agcgacaccc
     8461 gggcgagggc gagcgacaag cagaagggca acggcatcat catccagaac ggtgcccgag
     8521 cgatcatcac cgatacggcg gtcgtgagga atcgcgaggt gggccttatg at
//
LOCUS       DAOQUE010000058         8538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_9200_length_8538_cov_4.902747, whole genome shotgun sequence.
ACCESSION   DAOQUE010000058 DAOQUE010000000
VERSION     DAOQUE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9200_length_8538_cov_4.902747"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKH10_02925"
     CDS             complement(<1..410)
                     /locus_tag="PKH10_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT27109.1"
                     /translation="MRSLPIIFLLIVAFIFSFDLSCSTSKKSSTVPPAPKPKILSEKK
                     KTPPPPPPAKEEPASFSYQTLYFDFDSAAILYRDSTHLDRLAEFLINNKEISLVIAGH
                     ADERGSFDYNLSLGEQRAIAIRSELQKRGINPERV"
     gene            626..850
                     /locus_tag="PKH10_02930"
     CDS             626..850
                     /locus_tag="PKH10_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27110.1"
                     /translation="MASLVPRRKKAAQKVTFYLSDDLFELYRNNRERAKILNMTIDFT
                     GDFHKWFLDQNNFVKQQLDQLVAVKQEESE"
     gene            913..2652
                     /locus_tag="PKH10_02935"
     CDS             913..2652
                     /locus_tag="PKH10_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNT27111.1"
                     /translation="MFRIRRLADPDFPAEKKVIQQVQEIIRRQFPLLNSDKVDRLPDQ
                     LNDPLKYGFRASLFIAEDRRAQVKGFALLFYAPDLRFHYLDLIAADSFKTTGGVGGAL
                     YQRVREEVGRVEGLGLFFECLPDDESVCLDRTSIPQNIARLKFYERFGARPIIGTAYE
                     APVTEKDKCPPFLVYDPVKEGRVLLRRDAKRVVQAILERCYGDLCSPEYISLIVTSFG
                     DDPVRIRKPRYSKGKKIPAPSLGTASPPFHLVMSARHAIHHIRERGYVESPARLESIL
                     KDVKETSLFNFLSEEEFSEAHIRRTHDDKMVDFIKNAASWMEKGETLYPYIFPVRNPL
                     KIPNDPHVQAGYYCIDTFTPITSDIWAAARSAANCSLTAARLVLDEGGVTYALVRPPG
                     HHAERKVFGGFCYLNNVAVVSDFLSPHGRVAILDVDFHHGNGQQEIFYERSDVLTVSL
                     HGHPSFAYPYFSGFANEKGRGQGEGYNLNYPLAETTSFAEYRTTLSKAIQHIKKFNPA
                     FLVVALGLDTAKGDPTGTWRFRDGDFFLMGEDIGNMNLPTVVVQEGGYRTLNIGLNVR
                     RFFEGMASSRAPE"
     gene            2654..3505
                     /locus_tag="PKH10_02940"
     CDS             2654..3505
                     /locus_tag="PKH10_02940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT27112.1"
                     /translation="MKDRDARKNDSTGRKKTGTISADLLVKNDIFSTTRTLAEIRLLQ
                     KEATLTHEEPYLFLVHALFGAGHHLTEKEALSIWKKVIRHRGVLVKLLERPVNLRAVA
                     IDWLSLQKKNDRPFSAVVVSRRMLSQLTRESRHDLLTGLPNRRYFFELAMRAMQSRSG
                     KYGMVAYIDLNGFKSINDRLGHAQGDALLCLFAETALKTLRSRDIIARIGGDEFAVFL
                     LENDHRKGRAVLMRLRSVFEKESSLISGELPVSFSFGIVTIGKHSISKALEEADARMY
                     REKVAGT"
     gene            3583..4383
                     /locus_tag="PKH10_02945"
     CDS             3583..4383
                     /locus_tag="PKH10_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNT27113.1"
                     /translation="MSMIIFTDIDATLIGHDDYRYEEIRPLMRRLIEHRIPVVLVSSK
                     TFAETVPLHRELGLTEPFIVENGGGIAIPEGHPMAAESDGREQNGFLLEPLGVPYATI
                     RRFVVSRRMKGKITGFGDLTVSEVARLTGLSEGAAAAAKERLFSEPFLLKDRDDLEFV
                     IGQASEAGLAVTTGGRFHHLIGAGIDKGSAMGTMLERYHRYGVGAGPVIALGDGPNDL
                     PMLARADIAVLIPRPGMAHEPFEHPRLMRAEHPAPRGWRESVEILMDN"
     gene            4395..5642
                     /locus_tag="PKH10_02950"
     CDS             4395..5642
                     /locus_tag="PKH10_02950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT27114.1"
                     /translation="MAKKRFFSAMRDATRREIEQLEQVDIVVGIPCYFSEASVTHVIR
                     MAAAGLHRYFGGMRALILASDGGSTDDTRETIEKVAMNYPNVHKISSIYRGIPGKGSA
                     MREILEAGVFLKAKAILFFDSDLKSINPEWVQNLAGPLFSGYDFVAPQYRRYKFDGTI
                     TNTIVYNLTRALFGARIRQPIGGDFAVSRTLAKHYLDQDVWETDVARFGIDIWMTITA
                     VTGGFLACQARLGAKVHGEKDPASDLSGMFREVVGTVFQLMEEHEAFWKREHPEIEVP
                     SFGNFVGAEVKPFEIDEAALIEYFKVGYQNFAGVWERIIGPEDFAAVKQLAGSDCCPL
                     RLPDEAWVRIVYDYACALRATPVQRFKLLSTMVPLYYGRVASLVHALKDRDGDGAEAF
                     FEEQAALFERMKPYLIEKWGKGG"
     gene            5644..6858
                     /locus_tag="PKH10_02955"
     CDS             5644..6858
                     /locus_tag="PKH10_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNT27115.1"
                     /translation="MSDFFQNGVIATLQRIGERPLADIETELTGFSRQRAVILLLPAL
                     YSEFEGAAMPRIVEELRGVPYLKKIVLSLDKADEKQFREVKERMSGLSAECRVVWQDD
                     PDIRAIYDELDASGFNVRVPGKGRSVWLTLGYILADHETESCAIALHDCDIVNYSREL
                     LARLVYPIVHPALNFEFSKGYYARGTDRLYGRVTRIFYTPLLRSLTKILGNRPFLDFL
                     DSFRYALSGEFACIGELARSIRISPTWGLEVSMLSEVYQQANVNRVCQVEILDTYEHK
                     HRELSKEDPSTGLSRMAEDIAQALFRVLSQDGYVMSDSFFRTILSTYIQESRKAIEKY
                     NGLASINGLAYDRNGEVSASEVFVESIKRAQEEFARDPIGIPLMSAWVRVRAAIPDLQ
                     DRLFSIVENGNR"
     gene            6855..8423
                     /locus_tag="PKH10_02960"
     CDS             6855..8423
                     /locus_tag="PKH10_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium transporter"
                     /protein_id="HNT27116.1"
                     /translation="MRPLDWAILSLYIAVILGMGFLIGRRQRDQREYYVAGGTVPWWQ
                     VGLSLVANQVSAISLIGAPAFIAVKSGGGLKWLQYEMAVPLAMIPIIVVLVPAYWRHR
                     GFTIYGYLEDRFGPVVRRAVSAIFLVSRSMGTGVALLATSYVTAVCLDMPLPLTIVLV
                     GVVSVLYTIWGGIRADIYSDIVQLAVLWLAAIACGVIIAIQIPEGGWGLSSWHERLAV
                     FELGANGVTDGANFSFWPMLFGGLFLYLSYYGCDQTQAQRLLCTAGPRESAKALALNG
                     VLRFPLVLTYCAVGVLMLPFMAAHPAFGETVAALPPDYLMPTFFLHHVPTGFLGLIVA
                     GVFAASMSSIDSAVNSLSAAAWNDILLAMRPSLNDLRDTVKVRWSRGIAAFWGGMAIL
                     FALWLGGGHDTVIELVNKIGSAFYGPVAAVFVFGILSSRSTARGALAGLVAGVGANIY
                     LWLFHADRVSWMWWNLVGFAVCMAVGMLMSSCGGTEKAQTERAEPRPWRMVWLLAGWF
                     AVIVLVGVLIERAW"
BASE COUNT         1767 a         2313 c         2501 g         1957 t
ORIGIN      
        1 acccgttcag ggttgatgcc ccgtttctgc agttcgcttc tgatggcgat cgcccgttgt
       61 tcgccgagcg aaagattgta atcaaaactg ccccgctcgt cggcgtgtcc ggcgatgacc
      121 agcgatatct ctttattgtt tatgagaaat tccgctaacc ggtcaaggtg ggtgctgtcc
      181 cggtaaagga tcgcggcact atcgaaatcg aaatagaggg tctggtagga aaaagaggcc
      241 ggctcttcct tcgcgggcgg tggtggtggc ggagttttct ttttttcaga caggattttt
      301 ggtttaggtg ccgggggtac ggttgacgat tttttcgaag tagaacagga gagatcaaaa
      361 gaaaaaatga aggctacgat gagtaaaaaa attataggta acgatctcat aactccttcc
      421 tcctattagt tttttcaaag accattttct tggtccacaa ttaagattat aacagcagag
      481 attattgatg tcaacaattg tagatatttt tgtctataag ttatagatat tatgtctatt
      541 gcaaagcgct gcaggtgttg acattggccc gattttcaat atgatgcgcc cgttagttcg
      601 ttgacctgtg agtgaggatg ttgacatggc cagtctggtt cccagaagga aaaaagcggc
      661 tcaaaaggtg accttttatt tgagcgacga cctgttcgaa ctctatcgga acaaccgtga
      721 acgggccaag atccttaaca tgaccatcga tttcaccggc gatttccaca aatggttcct
      781 cgaccagaat aacttcgtca aacagcaact cgaccagttg gtggcggtca aacaggaaga
      841 gtcggaataa aaaagtggct gcacctggat tttcttataa attttcatga agttcgcaaa
      901 gaggactctc gtatgtttcg catccgaaga ttagcggacc cagatttccc ggctgaaaaa
      961 aaggtcattc agcaggtaca ggaaattatc cggcgacaat tcccgctact taattcagat
     1021 aaggtcgatc ggttaccaga ccagttgaac gatcccctaa agtatggctt ccgcgcatcg
     1081 cttttcatcg ccgaggatcg ccgcgcgcag gtaaaaggtt ttgccttgtt attctacgct
     1141 cccgatctcc gttttcatta tcttgatctt attgccgccg attcttttaa gaccactggt
     1201 ggcgtggggg gcgcactgta tcaacgcgtc cgtgaggagg ttggaagggt ggaggggctc
     1261 ggactttttt ttgaatgtct gcccgatgac gagagtgttt gcctcgacag aacgagtatt
     1321 ccgcagaata tcgcacgatt gaaattctat gagcgattcg gagctcggcc cattataggc
     1381 acggcctatg aagcccccgt gaccgaaaaa gacaagtgcc ctcccttttt ggtctacgat
     1441 ccggtaaaag aagggcgcgt tcttttgcgt cgggacgcca aacgggttgt tcaggcaatt
     1501 ctggagagat gttatggcga cctatgttct ccagagtata tctctttgat cgtgacatca
     1561 ttcggagatg acccggtccg catacggaag ccgagatatt caaaagggaa aaagataccc
     1621 gcgccctcgc tgggaacagc gagcccccct tttcatctag tgatgagcgc ccgacatgcc
     1681 atccatcata tcagggagcg aggctatgtg gagagtccgg cccgattgga aagtatcctc
     1741 aaggatgtga aggagacatc gttgttcaat ttcttatcgg aggaagagtt ctccgaggca
     1801 cacatccgca ggacgcatga cgacaagatg gttgatttca ttaaaaatgc cgcttcgtgg
     1861 atggagaaag gcgaaacact ttacccctac atcttcccgg ttcgcaaccc tttaaagatc
     1921 ccgaacgacc ctcatgtgca ggccgggtac tattgtatag acacctttac ccctataact
     1981 tctgatatct gggcggcggc gcgctccgcg gcgaactgct ccctgaccgc ggcgcgtctg
     2041 gtgctcgacg aagggggtgt aacctatgcg ctggttcgtc ctccggggca tcacgccgag
     2101 cgaaaagttt ttggtggttt ctgctatctc aataatgtgg ctgttgtttc cgattttctt
     2161 tccccccatg gtagggtagc catccttgac gtcgattttc atcacggcaa tggtcaacag
     2221 gagatctttt acgaacgatc agatgtgctg accgtctctt tgcacgggca tccctcattc
     2281 gcatatcctt acttctcagg gttcgcgaac gaaaagggaa ggggacaagg tgagggatac
     2341 aatcttaatt atccattggc agaaacgacg tctttcgccg aatatcgaac gacgctctcc
     2401 aaggcgatcc aacatatcaa gaaattcaac cccgcctttc ttgtggtagc gctggggctt
     2461 gataccgcaa agggtgaccc gaccggcacc tggcgttttc gcgatggaga cttctttctc
     2521 atgggagagg atatagggaa catgaacttg ccgacggtcg ttgttcagga gggaggatac
     2581 cgcaccctca atataggact caatgtccgc cgattcttcg aaggaatggc ttcttcccga
     2641 gcaccggagt gatatgaagg atcgcgatgc tcggaaaaac gactcgaccg gaagaaaaaa
     2701 gaccggcacg atatctgcgg acctgttagt aaagaacgat attttttcaa caacgaggac
     2761 attggcggag atacgccttt tgcaaaaaga agcaacgctg actcacgaag aaccctatct
     2821 ttttctcgtg catgcgcttt tcggcgcagg tcatcatctc accgaaaaag aggcgctctc
     2881 catatggaaa aaagtgataa ggcatcgggg ggttttggtc aagttattgg aaagaccggt
     2941 aaatcttcgc gcagtggcga tcgattggct ttctctacag aaaaagaatg atcgcccctt
     3001 ttctgcggta gtggtctccc gaagaatgtt atcgcaactc accagagaaa gccgtcatga
     3061 cctgttgacg gggcttccca atagacgtta tttctttgaa ttggctatgc gagccatgca
     3121 atcgcggtcc ggaaaatacg ggatggtcgc ctatatcgat cttaacggat tcaaatcgat
     3181 caacgacaga ttggggcatg cgcaaggcga tgcactatta tgtcttttcg ccgaaacagc
     3241 ccttaagaca ttaagaagtc gcgacataat tgcccggata gggggagatg aattcgccgt
     3301 attccttttg gagaacgacc atcgaaaggg gcgggcggtg ctgatgcgtt tgaggtctgt
     3361 ttttgagaag gagtcgtcac ttatctccgg ggagctcccc gtttcgttct ctttcgggat
     3421 cgtgacgata ggtaaacatt ccatatcgaa ggcgctggaa gaggcagacg cgcgcatgta
     3481 ccgcgagaaa gttgctggaa cttgacatgc tttgaaaggg aagatactat taaaacaacg
     3541 agggacggct cagcctagac actttcaaag gggttcgatc gtatgagcat gatcatcttc
     3601 accgatatcg acgccacgct gatagggcat gacgattacc ggtacgaaga gatacgaccg
     3661 cttatgagga gattgatcga acaccgtatc ccggtggtgc tcgtttcttc caagaccttt
     3721 gccgagacgg tcccgctgca tcgcgaactg ggcctcaccg agcccttcat cgtcgagaac
     3781 ggcggcggta tagcgatacc cgaaggccat ccgatggcgg cggaaagcga cgggagagaa
     3841 cagaacggat tccttcttga gccgctcggt gtcccgtacg cgacgatccg gcgttttgtc
     3901 gtttcgcgcc gcatgaaagg aaagatcacc ggtttcggtg acctgaccgt atcggaggtg
     3961 gctcgactga ccgggctttc ggagggggcc gcggcggcgg cgaaggagcg cctgttctca
     4021 gagcccttcc tgctcaagga tcgggacgat ctcgaatttg tgatcgggca ggcatcagag
     4081 gcggggcttg ccgtcaccac cggcggtcgt tttcatcacc tcatcggggc ggggatagac
     4141 aaggggtctg cgatggggac gatgcttgag cggtaccacc ggtacggtgt cggtgccggt
     4201 ccggtcatag cgctgggcga tgggccgaac gatctgccga tgctcgcgcg ggccgacatc
     4261 gcggtgctta tcccgcgacc gggcatggcg catgagccgt tcgagcatcc ccggcttatg
     4321 cgggccgagc atcctgcccc gcgcggttgg cgggaaagcg tagagatatt aatggataac
     4381 tgataggagt tcccatggcc aagaaacgat ttttttcggc gatgcgcgac gcgacgcggc
     4441 gagagataga acaactggaa caggtcgata tcgtggtcgg catcccctgc tatttcagcg
     4501 aagcatcggt gacccatgtg atccgaatgg cggcagcggg tctgcaccgc tatttcggcg
     4561 ggatgcgggc gctcatcctt gcttccgacg gaggatcgac cgatgacacc cgcgagacga
     4621 tagaaaaggt cgcgatgaac tatcccaatg tccacaagat atcgtcgatc taccgcggta
     4681 tccccggcaa aggctcggcg atgcgcgaga tactggaggc gggggtcttt ctgaaggcga
     4741 aggcgatact gttcttcgac agcgacctca aatcgataaa cccagagtgg gtgcagaatc
     4801 tcgcggggcc gctcttctcg ggctacgatt tcgtcgcgcc gcagtaccgt cgttacaaat
     4861 tcgacggcac catcaccaac accatcgttt acaacctgac ccgtgccctg ttcggcgccc
     4921 gcatccgcca gcccatcggc ggcgatttcg cggtgtcgcg cacactggcc aaacattacc
     4981 ttgaccagga tgtgtgggag accgatgtcg cccgcttcgg tatcgatatc tggatgacga
     5041 taacggcggt caccgggggc tttctcgcct gtcaggcgcg gctcggggca aaggtgcacg
     5101 gagagaagga cccggcatcc gacctgtcgg gcatgttccg ggaggttgtc ggcaccgtgt
     5161 tccaactcat ggaggagcat gaggctttct ggaagaggga gcatcccgag atagaggtgc
     5221 catccttcgg gaacttcgtc ggggccgagg tgaagccgtt cgagatagat gaagcggcgc
     5281 tcatcgagta tttcaaggtg gggtatcaga actttgccgg tgtctgggaa cggatcatcg
     5341 gtccggaaga tttcgccgcg gtgaagcaac tggccggaag cgactgctgc ccgttgcggc
     5401 ttcccgacga ggcgtgggtc cgcatcgtgt acgactatgc ctgcgcgctc agggcgacgc
     5461 cggtccagcg gttcaagttg ctctccacga tggtgccgct ctactatggc cgggtggcga
     5521 gtctggtaca cgcactgaag gatcgtgacg gcgacggtgc cgaggcgttc ttcgaggagc
     5581 aggcggcatt gttcgagcgg atgaaaccgt atctgataga aaagtgggga aaaggagggt
     5641 aacatgtctg atttcttcca gaatggcgtc attgcgacgc tccaacgcat cggcgagcga
     5701 ccgcttgccg acatcgagac cgaactgacg ggattctcgc gtcaacgtgc ggtgatcttg
     5761 ctgttgccgg cgctctacag tgaattcgaa ggggccgcga tgccccgtat cgtcgaagaa
     5821 ctccggggcg tgccgtatct caagaagatc gtgctctcgc tcgacaaggc cgacgagaaa
     5881 cagttccgtg aggtcaagga acggatgagc ggcctatcgg ccgaatgtcg cgtcgtctgg
     5941 caggatgacc ccgacattcg ggcgatctac gacgaactcg atgcctccgg cttcaatgtc
     6001 cgggtgccgg gaaaggggcg ttcggtgtgg ctcacgctcg ggtatatcct cgccgaccat
     6061 gagaccgaat cgtgcgccat agcccttcac gactgcgata tcgtcaacta ttcgcgcgaa
     6121 ctgctggcgc ggctcgtcta tccgatcgtc catccggcgc ttaatttcga gttcagcaaa
     6181 gggtattacg cccgcggcac cgaccggctc tacggccggg tcacccgcat tttttatacg
     6241 ccgttgctcc ggtcgctcac caagatcctc ggcaatcgcc cctttctcga tttcctcgac
     6301 tctttccggt atgcgctgtc gggcgaattc gcctgtatcg gcgaactggc tcgctcgata
     6361 cggatatctc cgacctgggg actcgaggtc tccatgctct cggaggtcta ccaacaggcc
     6421 aatgtgaacc gcgtctgtca ggtcgagata ctcgacacct acgagcataa gcaccgcgaa
     6481 ctttcgaaag aggatccctc gaccggtctt tcccggatgg ccgaggacat cgcgcaggcg
     6541 cttttccgcg tccttagtca ggatggctat gtgatgtccg actcgttctt ccgcacgatc
     6601 ctttcaacct acatacagga atcgcgcaag gcgatagaga agtacaacgg acttgcttcg
     6661 ataaacggac tcgcctacga ccggaacggc gaggtctccg cgagcgaggt gttcgtcgaa
     6721 tcgatcaagc gggcgcagga agagttcgct cgcgatccga tcggcatacc gctcatgtcg
     6781 gcatgggtcc gggtccgcgc ggctataccc gacctgcagg accgcctgtt ctccatcgtc
     6841 gagaacggga accgatgagg ccgctcgact gggcgatact ttccctgtat atcgcggtca
     6901 tcctcggtat ggggttcctc atcggtcgcc gacagcgcga ccagcgtgaa tactatgtcg
     6961 ccggcggtac ggtgccgtgg tggcaggtgg ggctgtcgct cgtcgccaat caggtgagcg
     7021 ccatcagtct catcggcgcc cccgccttca tcgccgtgaa aagcggcggc ggactcaaat
     7081 ggctccagta cgagatggcg gtgccgctcg ccatgatacc gatcatcgtc gtgctcgttc
     7141 ccgcctactg gcgtcatcgc gggttcacca tctacggcta tctggaggac cggttcggtc
     7201 cggtggtccg acgcgccgtc agcgcgatat tcctcgtgag ccgctcgatg gggaccgggg
     7261 tcgcgctgct cgcgacctcc tatgtgacgg cggtctgtct cgatatgccc ttgccactca
     7321 ccatcgtgct tgtcggcgtg gtgtcggtcc tttacaccat ctggggcggt atccgggccg
     7381 atatctattc cgacatcgtt cagttggcgg tgctttggct cgccgcgatc gcctgcggcg
     7441 tcatcatcgc gatccagata ccggaaggag gctggggcct ctcgtcgtgg catgaacggc
     7501 tcgcggtgtt cgaactcggc gcgaacggag tgaccgacgg ggcgaacttc tcgttctggc
     7561 ccatgctgtt cggcggattg ttcctctacc tctcctacta tggctgtgac cagacgcagg
     7621 cccagcggct cctctgcacc gccggtcccc gcgaatcggc caaggcgctc gcgctcaacg
     7681 gcgtcctgcg ctttccgctc gtgctgacct attgtgccgt cggggtgctg atgctcccct
     7741 tcatggcggc gcatccggcg ttcggcgaga cggtcgccgc cctgccgccc gactacctca
     7801 tgcccacctt cttcctgcat cacgttccga ccggctttct ggggctcatc gtcgccggcg
     7861 tcttcgccgc ctcgatgtcg tcgatagatt cggcggtcaa ttcgctttcg gccgccgcct
     7921 ggaatgatat cctgctcgcg atgcgcccct cgctgaacga cctccgcgac accgtgaagg
     7981 tgcgttggtc gcgcggtatc gccgcgttct ggggcggcat ggcgatactg ttcgcgttgt
     8041 ggctcggcgg cggtcacgac acggtcatcg aactggtcaa caagatcggg tcggcctttt
     8101 acgggccggt cgcggcggtg ttcgtgttcg gcatactttc ttcgcgctca acggcgcgcg
     8161 gcgcgctcgc cggtctcgtc gccggggtcg gtgccaacat atacctctgg cttttccacg
     8221 ccgatcgcgt cagttggatg tggtggaatc tcgtcgggtt tgccgtctgc atggcggtcg
     8281 gtatgctgat gtcgtcgtgc ggcggcaccg aaaaggcaca gacggaaaga gcggagcccc
     8341 gaccgtggcg catggtgtgg ctgcttgcgg gatggttcgc ggtgatcgtg ctggtcggcg
     8401 ttcttatcga gcgggcgtgg taatggtcag tgagcccgga ccggcgtcgc aaccgccttt
     8461 cttcagcgcc cctcgatggt gatgtcgccc gataccgttt tcacgacgac ctgtttttgc
     8521 gcccctttct cttctgac
//
LOCUS       DAOQUE010000059         8479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_9285_length_8479_cov_4.142925, whole genome shotgun sequence.
ACCESSION   DAOQUE010000059 DAOQUE010000000
VERSION     DAOQUE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9285_length_8479_cov_4.142925"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1699)
                     /locus_tag="PKH10_02965"
     CDS             complement(<1..1699)
                     /locus_tag="PKH10_02965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNT27117.1"
                     /translation="MLKKDEIYRVFVEESREILAALETDLLDLEKDINNEETINRVFR
                     AVHTLKGGSGMVEFSVLTDLAHEWENVLARLRVGEIKASPRLISLFLEAVDTLKRLIE
                     QPPVRVPEALRAEIARTIDQLKRYHGVETVSDGPAKRPTDGREKFGEKYLNIVLKFRP
                     DIYLAGTDPLLLIKELIEQGEIVRIRCDTDALPPLDQLRPDTLYLSWELVLRTEKPLS
                     AIENIFIFVRDENTILLDDVSHRFKDGVDMLYADKKLGEILEEEGLVDRRDLEEAIGE
                     QKRAGEILLEKKKLDSRALDEVLRRQQRSKEIAQTSTLRVDTGKLDKLVNLVGEMVIG
                     VARMSQTVQSGPLAHNRDMNDTLDHLERISRDVQEQVMRVRMVPVEATFRRFQRVVRD
                     IASDLGKRINLYLSGIETEIDKTVVEHLDDPLKHLIRNAVDHGIESPEERVKRGKPAD
                     GSVWLRAYQQEGKIIVEVEDDGSGIDRERLVRKAKEAGIALSAEMTDQELYGLLFLPG
                     FSTARKVTELSGRGVGLDVVKRNIENLRGAIEVYSEKGSGTLFQLKLPLTLAIIDGMR
                     "
     gene            complement(1707..2087)
                     /locus_tag="PKH10_02970"
     CDS             complement(1707..2087)
                     /locus_tag="PKH10_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT27118.1"
                     /translation="MNGKTIMIVDDSATLRASVNFVLSEAEYTVIQAKDGMDGLAKLR
                     DLQASGTKLDMIITDINMPNLDGIGFITEIKKSDYRYVPILVLTTESENAKKMEGKAA
                     GAAGWLVKPFKPEQLLWVVKKFVR"
     gene            complement(2077..2373)
                     /locus_tag="PKH10_02975"
     CDS             complement(2077..2373)
                     /locus_tag="PKH10_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNT27119.1"
                     /translation="MANFRLSRDIAFLGGDLTVNAAEALHNFLLTLQANQGRLTVDLR
                     GAEAWDSSSIQLLFAFIKSRPPRTVVWRNMPPEMAADIRLMGFGNLLNEVSHER"
     gene            complement(2373..2684)
                     /locus_tag="PKH10_02980"
     CDS             complement(2373..2684)
                     /locus_tag="PKH10_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNT27120.1"
                     /translation="MLTYTIEKRGGTMLITTEGRIDNEGAAAFQEALDRAFEANLPRI
                     SFDLAKLSFINSAGIGKLLLFYKRVKNRNGTVVITALNDDVNALFKAIRLDKLMPIER
                     S"
     gene            complement(2823..3359)
                     /locus_tag="PKH10_02985"
     CDS             complement(2823..3359)
                     /locus_tag="PKH10_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT27121.1"
                     /translation="MLQEMIFAGFGGQGVLSMGKLIAYAAMKEGKEVSWMPSYGPEMR
                     GGTAYAMVNISDEQISSPVVHAYDVVVVFNLPSLKKFESKVKKGGILIWESSVIKEPP
                     TRTDIKVYGVPAIEKSGSVLNEVKVMNMVTLGALIKANPIVKPETLLEALKDTLPPRH
                     HNMIPINKQAIELGMSLV"
     gene            complement(3372..4163)
                     /locus_tag="PKH10_02990"
     CDS             complement(3372..4163)
                     /locus_tag="PKH10_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT27122.1"
                     /translation="MKEGYELIYQKPSTLLPRRMHYCPGCLHSTLHKLIMEVVEEMGI
                     KEKTIGVSPVGCSVFAYDYMDVDMQQAAHGRAAALATAIKRMHPDKYVFSYQGDGDLA
                     AIGTAETIHACNRGENFTFFFVNNGIYGMTGGQMAPTTLEGQKTSTSPYGRDPKKVGQ
                     PLKMAEMVSQLPGVAYSERLSLNNPGNVRKAKKTIRKAFEMQQQGLGTTFLEFASNCP
                     SGWKCTPVQSLDWIDKQMSPYFPLGVFKDVTTGVEKCSCADKQAK"
     gene            complement(4165..5247)
                     /locus_tag="PKH10_02995"
     CDS             complement(4165..5247)
                     /locus_tag="PKH10_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNT27123.1"
                     /translation="MADIKFMKGNEALAEAAIRAGMHAYFGYPITPQSEVVEYLAREE
                     PKHKYVLLQAESEVASANMLYGASGAGARVMTTTSSPGYSLMQEAVSYIACAELPTVF
                     ANVQRGGPGLGTIQASQGDYFQAVKGGGHGDYRNIVLAPNSVQEMVDLTVLAFDLADK
                     YRAPALILSDGAIGQMMEKVVLPEQRPYRTPESYRAEKPWATMGKTADRERNVITSLN
                     IQSEQMEKVNIRLQAKYKEIEEKEVRFEAYHADDAEIVIVAYGVTSRITKKVVDLARA
                     EKLKVGLIRPITLWPFPKKKLDELAKKPAVKFFLSVEMSCGQMVEDVQLVSNGRKPVH
                     FYGRQGGIIPTAEEVLDVIKKHLKEK"
     gene            complement(5272..5496)
                     /locus_tag="PKH10_03000"
     CDS             complement(5272..5496)
                     /locus_tag="PKH10_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT27124.1"
                     /translation="MAAKGFVEISVEGCKGCGLCVHYCPVKCLDLNMSDTNSYGLHYA
                     FLKEPDKCIGCANCGVICPDAAITVYRKKN"
     gene            5675..7486
                     /locus_tag="PKH10_03005"
     CDS             5675..7486
                     /locus_tag="PKH10_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNT27125.1"
                     /translation="MMNLPNRKRPDGTAIGMIYELIQNSYAANTGKLMKITVPGRGDI
                     TLTYETTGGKKLDTILTPEGNGLAYDYTGALLATETWSGETAGTVGRTYNNDFRVSAL
                     TVNGVATAYTYDNDGLLTGAGDLVVTRDPQSGLVTGTTLGALTTAQTYTSFGELAHYE
                     AGTLYSYDFTERDRLGRIVEKVETIDGLAKTYRYGYDSAGRLATVWENGFVTGTYGYD
                     QNGNRTTHGGLAATYDDQDRMMTYGDNAYTYTVNGDILTKTDTQGTPDTADDELTGYT
                     HDALGNLTGVTLPDGTAITYVYDGRNRLIAKRVNGTVDYRLIYAGQLAPVAKVDASGA
                     TIEQYIYGLGVNAPDYILKDSVNYRLVKDHLGTPRLVVNATTGVVVKRIDLDEFGNIV
                     AETGTFDIPFGFAGGIRDTDTGLTKFGARWYDPETGRWLEKEPLGFRGGMNFYSYARN
                     NPVNKVDRTGLAPGDFYSNYDDARKDQVVDLIFEYTERWKANNGPYEIGSEIYVAYRY
                     NEDTQACEMGFSYTTPHDDDKRDDEVSINYVDGIVAFTHMHPRGSSILDSPTDTRIAD
                     KDRIIINAIRQSGGSWFVESYDPSKPPNERYHIEEHK"
     gene            7455..8288
                     /locus_tag="PKH10_03010"
     CDS             7455..8288
                     /locus_tag="PKH10_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27126.1"
                     /translation="MRGITLKSINKRTGFLLIFILTMAFSCEKKPVEYGDNENRDQYT
                     KKKSIVLPQWNEKNEIPANPHDAMAYLMKNLDKETVGSLGKCLNEEDAATLTAHNLGL
                     WIRNKWKLWGGSPLLSYFRERGIDNPDVISYLIVKWLWREIKGLGYDINSDIEDARKK
                     YPQKLDSHIEKVYVKKVTSPLNSEEILESILEIVPELDICLNFQLLEKIPIKNEYNLS
                     FHCCKNKKLTPIAYTRYDEKCLAEHISSKVNISQTTLKKNGALEVNIKTKKGTPVVSP
                     K"
BASE COUNT         1785 a         2382 c         2471 g         1841 t
ORIGIN      
        1 cgcgcatgcc gtcgatgatg gcgagcgtga gtggcagttt gagttggaac agggtgccgc
       61 ttcctttctc ggagtagacc tctatggcgc cgcgcaggtt ctcgatgttg cgcttgacca
      121 cgtccagtcc gacgccgcgc cccgaaagtt cggtgacctt ccgggcggtc gagaaaccgg
      181 ggaggaacag gagcccgtac agttcctgat cggtcatctc ggcgctgagg gcgatgcccg
      241 cctccttcgc cttgcggacg agccgttccc ggtcgatgcc ggaaccgtca tcctcgacct
      301 cgacgatgat cttgccttcc tgttggtagg cgcggagcca cacgcttccg tcggccggtt
      361 tgccgcgttt cacgcgctct tccggtgatt ctatcccgtg atcgaccgcg ttgcggatga
      421 ggtgcttgag cgggtcgtcg aggtgttcga cgaccgtctt gtctatctcg gtctctatgc
      481 cggaaaggta gaggtttatc cttttgccga gatcgctcgc tatatcgcgc accacccgct
      541 ggaaccggcg aaaggtcgcc tcgacgggga ccatgcggac ccgcatgacc tgctcctgca
      601 catcgcggct gatgcgctcc agatggtcga gcgtatcgtt catgtcgcgg ttgtgggcga
      661 gcggccccga ctgtacagtc tgcgacatcc gcgcgacgcc gatgaccatc tcgccgacga
      721 ggttcaccag tttgtcgagt ttgcctgtgt cgacgcgcag ggtcgaggtc tgggcgatct
      781 ccttcgaccg ctgttgacgt cgcaacacct cgtcgagcgc ccgtgaatcg agtttcttct
      841 tctcgagcag tatctcgccg gcccgtttct gctcgccgat ggcctcctcg aggtcgcgtc
      901 ggtccacaag accctcttct tcgagtatct cgcccagttt cttgtcggcg tacaacatgt
      961 ccacgccgtc cttgaagcga tgcgagacat cgtcgagcag gatggtgttc tcgtcacgga
     1021 cgaagatgaa tatgttctcg atcgccgaga gcggcttttc ggtccggagc accagttccc
     1081 agctcaggta cagcgtatcg ggtcgcaact ggtcgagcgg cgggagtgcg tcggtgtcgc
     1141 accggatgcg gacgatctcg ccctgctcga tcagttcctt tatcagcagg agcggatcgg
     1201 tgccggcgag gtagatgtcg ggccggaact tcaggacgat gtttagatat ttctcgccga
     1261 atttctccct gccgtcggtc ggtcttttcg ccgggccgtc ggataccgtt tcgacaccgt
     1321 ggtagcgctt caactggtcg atggtgcgcg ctatctcggc ccggagcgcc tccggcacgc
     1381 ggacgggcgg ttgttcgatg agccgcttga gggtatccac cgcctcgagg aagagcgata
     1441 tgaggcgcgg ggatgccttt atctcgccga cgcggagtcg cgccagcaca ttctcccatt
     1501 cgtgggcgag atcggtcagg acggagaatt cgaccatgcc ggagccgccc ttgagtgtat
     1561 ggacggctcg gaagacgcgg ttgatggtct cttcgttatt gatatccttt tcgaggtcga
     1621 gcagatcggt ctcgagcgcg gccagtatct cgcgcgactc ttcgacaaaa acgcggtaga
     1681 tctcgtcctt tttgagcatg cccgtcctat cgaacgaatt tcttcacgac ccacagcaac
     1741 tgttccggct tgaagggctt aacgagccac ccggcggcgc cggccgcctt cccctccatc
     1801 tttttcgcgt tctccgactc ggtcgtgagg acgaggatgg gaacatagcg gtaatcgctc
     1861 ttttttatct cggtgatgaa cccgatgccg tcgagattgg gcatgttgat atcggtgatg
     1921 atcatgtcga gtttggtgcc cgatgcctgc aggtcgcgga gtttcgcgag accgtccatg
     1981 ccgtccttgg cctggatgac ggtgtattcg gcctccgaca ggacgaagtt caccgatgcc
     2041 cgcagtgtgg ccgagtcgtc cacgatcatg atggtcttac cgttcatggg aaacctcgtt
     2101 gagcagattg ccaaagccca tcagccggat gtcggcggcc atctccggag gcatgttacg
     2161 ccatacgacg gtccggggcg ggcggctttt gataaaagcg aacaacaact gtatggagga
     2221 actgtcccac gcctcggcgc cgcgcaggtc gacggtgagc cggccctgat tcgcctgtag
     2281 cgtgagcagg aaattatgaa gcgcctcggc cgcgttgacc gtcagatcgc cgccgagaaa
     2341 ggcgatgtcg cgggaaaggc ggaagttcgc catcaggacc tctctatcgg catcagtttg
     2401 tcgagccgta tcgccttaaa gagggcgttc acatcgtcgt tgagggcggt gatgacgacg
     2461 gtcccgttgc ggttcttcac ccgtttgtag aacagcagga gtttcccgat gcctgcgctg
     2521 ttgatgaagg agagtttcgc cagatcgaag gatatgcggg gcaggttcgc ctcgaacgcg
     2581 cggtcgagcg cctcctgaaa ggccgcggcc ccctcgttgt ctatccgtcc ctcggtggtg
     2641 atcagcatgg tgccgccgcg tttctctatc gtataggtca gcatcgtctc ctctcgtggg
     2701 tcgggacagg ggcgcatcgg ggcgcgccgg tccgcaactt gatggaacgg aagactatac
     2761 ccataaagaa tttttaggtc aacatttttg ggttctttgc ggtcggggcg gcgcggggga
     2821 cgctagacga ggctcatacc cagttctatc gcctgtttgt tgatgggtat catgttgtgg
     2881 tggcggggcg ggagcgtgtc cttgagcgcc tcgaggagcg tttcgggctt cacaatgggg
     2941 ttcgccttga tgagcgcgcc gagcgtcacc atgttcatga ccttgacctc gttcaggacc
     3001 gagcccgatt tctcgatggc cgggacgccg tagaccttga tgtccgtacg ggtcggcggc
     3061 tctttgatga ccgaactttc ccagatgagg atgccgccct tcttgacctt tgactcgaac
     3121 ttcttgagcg aggggaggtt gaagaccacg accacatcgt aggcgtggac gaccggcgac
     3181 gatatctgtt cgtcgctgat gttgaccatc gcgtaggcgg tgccgccgcg catctcgggg
     3241 ccgtacgacg gcatccaact gacctctttg ccctccttca tcgcggcgta ggcgatcagt
     3301 ttgcccatcg aaagaacgcc ctgcccgccg aacccggcga atatcatttc ctgtaacata
     3361 tctgcgcctc cttacttggc ctgtttatct gcgcacgaac acttctcgac gccggtcgtc
     3421 acatccttga agacgccgag cgggaagtag ggcgacatct gcttgtctat ccagtcgagc
     3481 gactgcaccg gcgtacattt ccagcccgac gggcagttcg aggcgaattc aaggaaggtg
     3541 gtgccgagtc cctgctgttg catctcgaac gccttgcgga tggtcttttt cgccttgcgg
     3601 acattgcccg gattgttgag cgagagccgt tcggaatagg cgacgccggg aagttggctg
     3661 accatctcgg ccatcttgag cggctgaccg acctttttcg ggtcgcgacc gtagggcgag
     3721 gtcgaggtct tctggccttc gagcgtcgtc ggggccatct ggccgcccgt catgccgtag
     3781 atgccattgt tgacgaagaa gaaggtgaag ttctcgccgc ggttgcaggc gtggatcgtc
     3841 tcggcggtgc cgatcgccgc gaggtcgccg tcgccctgat aggagaagac gtacttgtcg
     3901 gggtgcatcc gcttgatggc ggtggcgagc gctgcggcgc gaccgtgggc cgcctgctgc
     3961 atgtcgacat ccatgtagtc gtaggcgaag accgaacagc cgaccgggct cacgccgatg
     4021 gtcttctctt ttatgcccat ctcctcgacc acctccatga tgagtttatg gagcgtcgaa
     4081 tggagacagc cggggcagta gtgcatgcgg cgcggcagga gcgtcgaggg tttttggtag
     4141 ataagttcgt atccttcctt catcttattt ctccttcagg tgtttcttga tgacatcgag
     4201 cacctcttcg gcggtcggga tgatgccgcc ctgacggccg tagaagtgga ccggcttgcg
     4261 gccgttggaa acgagttgca catcctcgac catctgaccg caactcatct cgaccgacag
     4321 gaagaacttg acggccggct ttttcgccag ttcgtcgagt ttctttttcg ggaagggcca
     4381 cagcgtgatg gggcggatga ggccgacctt cagtttctcg gcccgcgcga ggtccacgac
     4441 cttcttggtg atgcggctgg tcacgccgta agcgacgatg acgatctcgg cgtcgtcggc
     4501 atggtaggcc tcgaaacgga cctccttctc ctctatctcc ttgtatttgg cctgcaggcg
     4561 gatgttcacc ttttccatct gctcagactg gatgttgagc gaggtgatga cattgcgctc
     4621 gcggtcggcg gtcttgccca tcgtcgccca cggcttttcg gcccggtacg attcgggggt
     4681 gcggtagggg cgctgttcgg gcaacacgac cttttccatc atctggccga tggcgccgtc
     4741 cgaaagtatg agcgccgggg cgcggtattt gtcggccaga tcgaacgcga gcaccgtcag
     4801 atcgaccatc tcctgtacgc tgttcggcgc gagcacgatg ttgcggtagt cgccgtggcc
     4861 gccgcctttc accgcctgaa agtagtcgcc ctgcgaggcc tgaatggtgc cgaggccggg
     4921 gccgccgcgc tggacattgg cgaagaccgt cggcagttcg gcgcaggcga tgtaggatac
     4981 cgcctcctgc atcagcgaat agccgggcga actggtcgtc gtcatcacgc gcgctccggc
     5041 gcccgaagcg ccgtagagca tgttggccga cgccacttcc gattcggcct gcaagaggac
     5101 atatttatgc ttcggctcct cgcgggcgag gtactccacg acctccgatt gcggggtgat
     5161 gggatagccg aaataggcgt gcatgccggc gcggatagcc gcttcggcga gcgcctcgtt
     5221 gcctttcatg aatttgatgt cagccactgc ttcctccttc tattcgttcc gttagttctt
     5281 cttgcggtac accgtgatcg ccgcgtcggg gcagatgacc ccgcagttgg cgcacccgat
     5341 gcacttgtcg ggctccttga ggaatgcgta gtgcagaccg taggagttgg tgtccgacat
     5401 gttcaggtca aggcacttga ccgggcagta gtggacgcag aggccgcacc ccttgcaccc
     5461 ttcgaccgag atctcgacaa aacctttcgc cgccattatt acctccgaaa gtataaggac
     5521 cataagaacc ctacgccggc caccctagca tgccggaaaa caaaatgcaa gccgagaggg
     5581 gtcagtcctt cattttacgg tttgagcaga aattttccat ttgcaaaaga gagcgaacgg
     5641 gctatgatga caacatcaaa gggggccagt cgtgatgatg aacttgccga acagaaaacg
     5701 ccccgacggt accgccatcg gcatgatcta tgaactcatc caaaatagct acgccgccaa
     5761 caccggcaaa ctcatgaaga tcaccgttcc cggcagaggc gacataacgc tcacctacga
     5821 aacgaccggc ggcaagaagc tcgacaccat cctcaccccc gaggggaacg gactcgccta
     5881 cgactacacc ggcgctctgc tcgccaccga gacatggagc ggagagacgg ccgggaccgt
     5941 gggccgcacc tacaacaacg acttccgcgt gagcgccctt accgtgaacg gcgtcgccac
     6001 cgcgtacacc tacgacaacg acggcctcct caccggggcg ggcgacctcg tcgtcacccg
     6061 cgacccacag agcggcctcg tgaccgggac gacgctcggc gccctgacca ccgcgcagac
     6121 ctacacctcg ttcggcgaac tcgcccacta cgaagcgggc acgctctaca gttacgactt
     6181 caccgagcgg gaccgactcg ggcgcatcgt cgagaaggtg gagaccatcg acggccttgc
     6241 aaaaacctac cggtacggct atgattcagc ggggcggttg gcaacggtgt gggagaacgg
     6301 cttcgtgacg ggcacctacg gctacgatca gaacgggaac cggacgacgc acggcggcct
     6361 tgcggcgacc tacgacgatc aggaccggat gatgacctac ggcgacaatg cttacactta
     6421 caccgtcaac ggcgacatcc tgaccaagac cgacacgcag gggacccccg acacggccga
     6481 cgacgaactg accggctaca cccacgatgc cctcggcaac ctgaccggcg tcacgctccc
     6541 cgacggcacc gcgatcacct atgtctatga cggcaggaac cggctcatcg ccaagcgcgt
     6601 gaacggcacg gtggattacc gcctcatcta cgccggacaa ctcgcccccg tggcgaaggt
     6661 tgacgccagc ggcgctacca tcgaacagta catctacggc ctcggcgtca acgcgcccga
     6721 ctacatcctc aaagatagtg tgaactaccg gctcgtcaag gatcacctcg gcacaccgcg
     6781 ccttgtcgtg aacgccacga cgggcgttgt ggtgaaacgc atcgacctcg atgagttcgg
     6841 gaacatcgtc gcggagacgg ggaccttcga catcccattc ggcttcgcgg gcggcattag
     6901 ggacaccgac accggcctga ccaagttcgg cgcgcggtgg tatgaccccg agacggggcg
     6961 gtggctggag aaggaaccgc tggggttcag gggagggatg aatttctata gttatgctag
     7021 gaacaatccc gtgaataagg tagatcggac cggactggca ccgggcgatt tctacagcaa
     7081 ttatgatgat gcgagaaaag atcaggtagt agacttgata tttgaatata ctgaaagatg
     7141 gaaggccaat aatggtccct atgaaattgg ttcagagata tatgttgcgt atagatacaa
     7201 cgaagatacg caggcatgcg aaatgggatt ttcttacacg acaccgcacg acgacgataa
     7261 aagagatgac gaggtaagca ttaattatgt tgatggaata gttgctttca cgcatatgca
     7321 tccacgaggt tcgagcattt tagattcacc aacggacact cgcattgccg ataaggatcg
     7381 aataataata aatgccataa ggcaatccgg aggttcttgg tttgttgaat cgtatgatcc
     7441 atcaaaacca cccaatgaga ggtatcacat tgaagagcat aaataaaagg acagggtttc
     7501 ttcttatttt tattctgaca atggcctttt cgtgcgagaa gaaacctgtt gaatatggcg
     7561 ataatgaaaa tagggatcag tacacgaaaa agaagagcat cgttttaccc caatggaatg
     7621 aaaaaaacga aatacccgct aaccctcacg acgcaatggc ctatcttatg aaaaatctcg
     7681 ataaagaaac cgttggttct ctaggcaaat gtcttaatga ggaagacgcg gccactctga
     7741 ctgctcacaa tcttggtctt tggataagaa ataaatggaa attatggggg gggtcgcctt
     7801 tgctttcata tttcagagaa agaggaattg acaatcctga tgtgatttca tacttgattg
     7861 tgaaatggct ctggcgcgag attaaaggtc ttggttatga tattaattca gacatagaag
     7921 atgctcggaa aaaatatcca caaaaattag attcgcacat agagaaagtc tatgttaaaa
     7981 aggtgacatc gcctctgaac agcgaggaga tattagaaag cattttggaa atagttcctg
     8041 aacttgatat atgtcttaat ttccaattgc tggaaaaaat acccattaaa aatgaatata
     8101 atctctcttt ccattgttgc aagaacaaaa aactaactcc aatcgcttat acccgttacg
     8161 atgaaaaatg cctagcagaa catatatcaa gcaaagtgaa tataagtcaa actacgctta
     8221 aaaagaatgg ggcattggag gtaaacatca aaacgaaaaa aggaacccct gttgtttctc
     8281 caaaatgaga gcacaaccat gaaccgtcca tcgtccgtcg gcgtctttga caacatgaaa
     8341 ggcctcggcg gcaagaaact cgccggcata accacctgtc ctctgtcaga tcatcatcgg
     8401 acagcgatat tcgatagtta atcgaaaggt tcgaaatcat cgggaacctg aaaatcgcgc
     8461 caaacaggtc tgtcaaaga
//
LOCUS       DAOQUE010000060         8390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_9406_length_8390_cov_4.953929, whole genome shotgun sequence.
ACCESSION   DAOQUE010000060 DAOQUE010000000
VERSION     DAOQUE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9406_length_8390_cov_4.953929"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKH10_03015"
     CDS             <1..638
                     /locus_tag="PKH10_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT27127.1"
                     /translation="FGLVVWALTSGAAGKTSVLTYTMPFWVMLIAWPVLRERPSPLQW
                     GGIALTFVAIALSQSGKRSGRIGTGLILMFLAVGCAELSNKVFQQYALPQYKELFLFA
                     VFFTAFLIATAALCKSRQGPFDKGPEGRNVPFTQEFFVGLAVGVPNLFSSYFLIQAFA
                     TLKASVVFPFYSAGSIALITVGGVALFGERPTRRELAAIAIVLAAIVLMGG"
     gene            716..2143
                     /locus_tag="PKH10_03020"
     CDS             716..2143
                     /locus_tag="PKH10_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNT27128.1"
                     /translation="MLLLFACLIILAVYSTKISSRFGVPLLLLFLAIGLIVGSDVLNL
                     FYFSDAGLTKKIADILLIFILFDGGFRTSKQQFDPVSAPASVLATFGVVLTAVLLGAL
                     IHLLLGFDLLYAMLVASIISSTDAAAVFMITKENPLKPRLSATLNVESAANDPMAILL
                     TLAFMGLATGTSALTARFLLDLLWQFSGGILFGLLFAYAARFLFDRLESENRGNYYVL
                     IVGVVLLTYGVADLAKANGIIAVFFMGYRLGNSEFAAKRGVSHFMEGIATFGNVSLFL
                     MLGLLAFPREFVHIWKEGLLVAALLIFAARPAAVFLSTASFGYSVKEKLFLSWGGIKG
                     AVPVVLATYPAAFNLDPHGMIFNLIFFVVFLSCLLQGMTLAPLARRLKLNAPKTPVSP
                     YTVEIHGTRKSDIDMFEFRIPRDAPCVGRKIAEISLGRDVLISSIIRDERIILPKGST
                     ELKENDILFILAHADRIKEISEKLQ"
     gene            complement(2160..3785)
                     /locus_tag="PKH10_03025"
     CDS             complement(2160..3785)
                     /locus_tag="PKH10_03025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNT27129.1"
                     /translation="MAEQIMAVPLGGLREVGKNCLLLIYHKKALMVDCGMGFPGNDYL
                     MDDDFFIPDFDALATLGVTLEGVVITHGHEDHIGAIPYLLRDHDVPVYLTEFPAGLLK
                     ERMGKVARYQNLVVLPAKRKAPIEVGPFTVRMLPVPHSIPEAKGLLIEAGPFKLLHTG
                     DFKCDGSGHSPFYKKVPEDIDMMFVDSTNIEREGVSKQEPELIPNLRRIIAGAKGRII
                     VTAFSSNIERIKNILAVAQETGRTVGFMGRSVRTYTRIAQQLGYLELPGSIVTDESQF
                     STIPDKKLLMIVTGSQAEPRSIMKRLSLDMLKAVPLKYGDTILFSSKIIPGNETAIGR
                     MMDSLVERGVNLFYEATANIHASGHGRRDDLLLAAREVNPSLIVPVHGHLRFIDHHCR
                     VLEKEGFSSRLISDGDVLVYTKKDSELRQRIDLQKRIVSNYSTELVDIETIRERKRLA
                     RSGGMTVVLSVNLIAGKLVTPPRFLSLGIASASAMKAIERALRDLIYDYFADKKNAAR
                     EPEEIEDDIRVIVRRYLTELTDKKPVVTVVVTE"
     gene            3970..6300
                     /locus_tag="PKH10_03030"
     CDS             3970..6300
                     /locus_tag="PKH10_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27130.1"
                     /translation="MKKILLLSLLSFAFLLGAAEVAAPRIAFKKGVPSFVTGLTVTLP
                     ADDTAVTRWLTRDFLPTYLPRTAAKPTLVRQVGHGDHRLFRYSYAVNGVPVEGAFTTV
                     ALRDGAVYRVTNSLGVIPSIDTTPVLSTADAARVAFTAQTGRSILSAPRHWAEKRIVR
                     HHGSWRLAWKVRLTPVSLADGRFYYIDARDGALLGGGNDTRFAEPTDMAKVFETNPKR
                     NKTPIEVELPWIDPTDGKLTSAPDGEGVRAIVAANCIDEGKTVEVTGYGTLAICTTTQ
                     IADKNVNGNFTYEDWDDGVAMKFDADDIYPEVSMYYHASKIYKFLRDLELDGFHYLSG
                     HNAGSANPSPLIVVANFQMPQQGGGLAPMDNAFFSPVQPGFTDIFFADFPYQGDMLVF
                     GQGTKTDFAYDGDVIYHEFGHAVEMATSGLSPTPYPDKYGFSNVPISINEGIADSFSF
                     IMSQDACLGEYASEGLAALAGYEKGEDGFYCMRRADNGRLVNEDFTGESHEDGLPFVG
                     MNWALYQEGLKNDLTREDFARLFIRMLLSFADPEASPQDYADIMLSEVENDPKFAPLK
                     DAVAALIEERGFNEKVRARDITRPVTYIMSGGTSTAYGAPTSSFTADVSGDEMKIAPA
                     YVQFYFDMPACMDTLTVTGRGVSTSQSGGTAPRYWLFVRKDEPVTYTIDDYPVVVALD
                     TVVEPVDNIYELKNLEAGARYYLHFVNVASSEGVVAYISANATRSSADQCVENPDDVV
                     PDDETTDELADADTVIVPDEADTKKGSSGGCSLILF"
     gene            complement(6305..7324)
                     /locus_tag="PKH10_03035"
     CDS             complement(6305..7324)
                     /locus_tag="PKH10_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNT27131.1"
                     /translation="MRKQLVVFGSFLFLFMVSCSAPVDEPVDEESFTPDTVVVDTAES
                     ENSLPETDPAVGETDDTDLFAPEEDALPDEGEVVEDGPMLDDAVTFDEDDIFVPDSDA
                     TSTCWEPEVLIAIDRTLSMRTAVDGVTKWDMARQAIAAIGEKFASGIYFGLEFFPKTV
                     EGCYTIAEIEAGSKVNNVQCLEGEVVVPPAYDNGELINSTMESAKLCRSSPIKSALLS
                     ARDFFVAHPPSPAERKQFVILLTDGKETCEKATLSCGAQELLAAGVKTYVVGFGGAVN
                     PDYLNHLVCAGGTAPDPNACLTGTSGCTEAYLGIDPLYFTAEDGDQLLLQLDEIAAKL
                     AECQS"
     gene            7540..>8390
                     /locus_tag="PKH10_03040"
     CDS             7540..>8390
                     /locus_tag="PKH10_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015206222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNT27132.1"
                     /translation="MKRIVGLAAVATAAAVVFFACKVGEVTKEAHENPDEGIVRFDDS
                     SVVQKIKELEKSAPVEKPADAVTTLGDEGRQNDKKSFKKEAQGSSRRSMDGAVFGSGG
                     IGTRGTGVGGGGSVGYGYGYGSITVHAYGRAAGAAVVQSYTYDTEQYEGKEEEGFRSA
                     LHDPLSTFSIDVDTASYANVRRFLTTDQLPPVDAVRIEELINYFSYRYPEPQGSDPFS
                     VTTALADCPWAPGHLLLQVGLKGKEIAAGKMPPANLTFLIDVSGSMNAPNKLPLLQKA
                     FSLLVKEL"
BASE COUNT         1524 a         2675 c         2504 g         1687 t
ORIGIN      
        1 acttcggcct cgttgtctgg gcgctcacca gcggcgccgc gggaaagacc tcggtgctca
       61 cctacaccat gccgttctgg gtgatgctga tcgcctggcc ggtgctgcgc gagcggccat
      121 cgccgctgca gtggggcggc atcgcgctca ccttcgtcgc catcgcgctg tcacagagcg
      181 gcaagaggag cggtcggatc gggaccgggc tcatcctcat gttcctcgcg gtagggtgcg
      241 ccgaactgtc gaacaaggtg ttccagcagt acgccctgcc gcagtacaag gaactcttcc
      301 ttttcgccgt gttcttcacc gcgttcctca tcgcgacggc ggcgctatgc aaatcccgtc
      361 aaggtccctt tgacaaggga ccggaaggaa gaaatgtccc tttcacacaa gagtttttcg
      421 tggggttggc cgtcggcgtg cctaacctgt tctcgagtta tttcctgata caggcctttg
      481 cgaccctgaa ggcctccgtc gttttcccgt tctacagcgc cggtagcatc gccctcatca
      541 ccgttggcgg cgtagcgctt ttcggcgaac ggccgacacg gcgcgaactg gccgccatcg
      601 ctatcgtcct tgccgccatc gtgctgatgg gcggctgaaa cggaaagagc cctttccttc
      661 ttgccttatc ggtacgcttc tgatagattt caccgatcga cccgaaggag acgagatgct
      721 tctgcttttc gcgtgcctga taatcctcgc cgtctactcg accaagataa gttcccgctt
      781 cggcgttccg ctcctgctcc tcttcctcgc gatcggcctg atcgtcggta gcgatgtcct
      841 caacctcttc tatttcagcg atgccggact gaccaaaaag atcgccgaca tcctgctgat
      901 cttcatcctg ttcgacggcg ggttccgcac ctccaaacaa cagttcgatc cggtctccgc
      961 ccccgcctcg gtactcgcca ccttcggcgt ggtcctcacg gcggtcctgc tcggcgcatt
     1021 gatacatctt ctcctcggct tcgatcttct ctacgcgatg ctggtcgcct ccatcatctc
     1081 ctcgaccgac gcggcggcgg tgttcatgat caccaaagag aacccgctca agccccggct
     1141 ttcggcgacg ctgaatgtgg aatcggccgc caacgacccg atggcgatac tgctgacgct
     1201 cgcgttcatg gggctggcga ccgggacctc cgccctgacg gcccggttcc tgctggacct
     1261 gctctggcag ttctccgggg gcatcctgtt cggccttttg ttcgcctatg ccgcgcggtt
     1321 cctgttcgac cgactggaat cggagaaccg cgggaattat tatgtgctga tcgtcggcgt
     1381 ggtactgctg acatatggcg tggccgatct ggctaaggcc aacggcatca tcgcggtctt
     1441 tttcatgggg tatcgtctgg gcaacagcga gttcgccgcc aaacgcgggg tcagtcattt
     1501 catggaaggc atcgcgacct tcggcaatgt ctcgctgttc ctcatgctcg gtcttttggc
     1561 ctttccccgg gaattcgtcc acatctggaa ggagggtctg ttggtcgcgg cgctcctgat
     1621 cttcgccgcg cggcccgcgg ccgtctttct ttcgaccgct tccttcgggt attcggtaaa
     1681 ggaaaaactg ttcctttcgt ggggaggcat caagggcgcc gtccctgtcg ttctggccac
     1741 ctaccccgcc gcgttcaatc tcgatccgca cgggatgata ttcaacctca tcttcttcgt
     1801 cgtattcctt tcgtgccttc tgcaggggat gacgctggct ccgctcgcac ggcggttaaa
     1861 actcaatgcg ccaaagaccc cggtttcgcc ctacaccgtc gagatccacg gcacgcggaa
     1921 aagcgacatc gacatgttcg aattccgcat accgcgggac gccccctgtg tgggcaggaa
     1981 gatagcggag atatcgctgg gccgcgatgt gctgataagt tccattatca gggacgagcg
     2041 catcattctc cccaaaggca gtaccgaact gaaggagaac gacatcctgt tcatcctcgc
     2101 ccacgccgac cggataaaag agatatcgga aaagttgcag tagcccccgt gaccggggct
     2161 cactccgtga cgaccaccgt cacgaccggc ttcttgtcgg tgagttcggt caggtagcgg
     2221 cgtacgatga cgcggatatc gtcctctatc tcctccggct cgcgcgcggc gttctttttg
     2281 tcggcgaaat aatcgtatat gagatcgcgg agcgcccgtt ctatcgcctt catggcgctc
     2341 gccgaggcga tgcccagaga gaggaagcgg ggcggggtga ccagtttccc cgcgatgagg
     2401 ttgaccgaca gcaccaccgt catgccgccg gagcgggcaa gccgtttccg ctcgcgtatc
     2461 gtctcgatgt ccaccagttc ggtgctgtag ttgctcacga tgcgtttctg caggtctatc
     2521 cgctggcgca gttccgagtc cttcttggtg tagacgagca catcgccgtc cgatatgagc
     2581 cgggaagaga acccctcctt ctcgagcacg cggcaatgat ggtcgatgaa acgcaggtgc
     2641 ccgtggaccg ggacgatgag cgaggggttc acctcgcgcg ccgcgagcag aaggtcgtcg
     2701 cgtcgcccgt ggcccgaggc gtggatgttg gcggtcgcct cgtagaagag gttcaccccc
     2761 ctctcgacga gcgaatccat catgcggccg atggcggtct cgttgcccgg tattatcttc
     2821 gacgagaaca gtatcgtgtc gccgtacttg agcggcaccg ccttgagcat gtcgagcgac
     2881 agccgtttca tgatggagcg cggttcggcc tgcgagccgg tcacgatcat gagcagtttc
     2941 ttgtccggta tcgtggagaa ctgcgactcg tcggtgacga tcgaaccggg cagttcgaga
     3001 tagccgagtt gctgggcgat gcgggtgtag gtgcggacgc tgcgacccat gaacccgacg
     3061 gtgcggcccg tttcctgcgc gacggcgagg atgttcttta tccgttcgat gttcgacgag
     3121 aaggcggtga cgatgatgcg ccccttcgct ccggcgatga tccggcggag gttcgggatg
     3181 agttccggct cctgcttcga gaccccttcc cgctcgatgt tggtcgaatc gacgaacatc
     3241 atgtcgatat cctcgggcac cttcttgtag aacggcgagt ggcccgagcc gtcgcacttg
     3301 aagtcgccgg tatggagcag tttgaacggc cccgcctcga tgagcagtcc cttcgcctcc
     3361 ggtatgctgt gcggcaccgg gagcatccgg acggtgaacg ggccgacctc gatgggcgct
     3421 ttgcgcttgg ccgggagcac cacgagattc tgataccgcg ccaccttacc catccgctcc
     3481 ttgagcaggc ccgccgggaa ctcggtgaga tagaccggca catcgtggtc gcgcagcagg
     3541 tacggtatcg ccccgatgtg gtcctcgtga ccgtgcgtga tgaccacccc ttcgagcgtg
     3601 acgccgagcg tcgccagcgc gtcgaagtcg gggatgaaga agtcgtcgtc catcagatag
     3661 tcgttgcccg ggaagcccat accacagtcc accatgagcg cctttttgtg gtagataagg
     3721 agcaggcaat tcttgccgac ctcgcgcaac ccgccgagcg gcaccgccat gatctgttca
     3781 gccatcgcac acctcgcgca caagcaaacg aacggcgcat cctagcacag gtcccgtccg
     3841 ggagcaatat aaacacgctt cccctactcc gccgatatca ctgagacatt aaccccggaa
     3901 tgttctttgc tcttttaaga gtcatgaggt gtctttgcgc gaaccactga tatataacgg
     3961 agcatcgtca tgaaaaagat cctgcttttg tcgcttcttt cctttgcgtt cctgctcggc
     4021 gccgccgagg tcgccgcgcc gcggatcgcg ttcaaaaagg gcgtcccctc cttcgtcacc
     4081 ggcctcacgg tcaccctgcc ggccgacgac acggcggtca cccgctggct cacccgtgat
     4141 ttcctgccga cctatctgcc ccgcaccgcc gcgaaaccga cgctcgtgcg gcaggtcggc
     4201 cacggcgatc accgcctgtt ccggtatagt tacgcggtga acggcgtgcc ggtcgagggg
     4261 gcgttcacca ccgtcgccct gcgtgacggc gcggtctacc gcgtgaccaa ctcgctcggc
     4321 gtgatcccct ccatcgacac gacaccggtc ctctcgaccg ccgacgccgc gcgcgtcgcc
     4381 ttcaccgcgc agaccggccg gtccatcctc tccgcgccgc gtcactgggc cgagaaacgg
     4441 atcgtgcggc accacggctc gtggcgcctc gcgtggaaag tgcggctgac cccggtgtcg
     4501 ctcgccgacg gccggttcta ctacatcgac gcccgcgacg gcgctctgct cggcggcggt
     4561 aacgacacgc ggttcgccga gccgaccgac atggccaagg tcttcgagac caaccccaag
     4621 cggaacaaga cccccatcga ggtcgaactc ccgtggatcg atccgaccga cggcaaactg
     4681 acctcggctc ccgacgggga aggggtgcgc gccatcgtcg ccgccaactg catcgacgaa
     4741 ggaaagaccg tcgaggtgac cgggtacggc acgctcgcca tctgcaccac cacgcagata
     4801 gccgacaaga atgtcaacgg caatttcacc tacgaggact gggacgacgg ggtggcgatg
     4861 aagttcgacg ccgacgacat ctatcccgag gtctcgatgt actaccatgc ctcgaagata
     4921 tacaagttcc tgcgcgacct cgaactcgac gggttccact acctgagcgg ccacaacgcc
     4981 gggtcggcca acccgagtcc tctcatcgtc gtcgccaatt tccagatgcc ccagcagggg
     5041 ggtggcctcg cgccgatgga caacgccttc ttttcgccgg tccagccggg gtttaccgac
     5101 atcttcttcg ccgatttccc ctatcagggc gacatgctcg tcttcggcca ggggaccaag
     5161 accgacttcg cctacgacgg tgatgtgata taccacgagt tcggtcatgc cgtcgagatg
     5221 gcgacctcgg gactttcgcc gacgccctac cccgacaagt acggcttctc gaatgtcccc
     5281 atcagcatca acgaggggat cgccgacagc ttctccttca tcatgtcgca ggacgcctgt
     5341 ctcggcgagt acgcctccga ggggctcgcc gcgctggcgg gctacgagaa aggcgaggac
     5401 gggttctact gcatgcgccg cgccgacaac ggtcgtctcg ttaacgagga cttcaccggc
     5461 gaatcgcatg aggacgggct ccccttcgtc ggtatgaact gggcgctgta tcaggagggg
     5521 ctcaagaacg acctgacccg cgaggatttc gcgcgcctct tcatacggat gctcctttcc
     5581 ttcgccgatc ccgaggcaag tcctcaggat tacgccgaca tcatgctctc cgaggtcgag
     5641 aacgacccga agttcgcccc gctcaaggac gccgtcgcgg cgcttatcga agagcgcggg
     5701 ttcaacgaga aggtccgggc ccgcgacatc acccgtccgg tcacctacat catgtcgggc
     5761 ggcacctcga cggcctacgg cgcaccgacc agttccttca ccgccgatgt gtctggcgac
     5821 gagatgaaga tagcccccgc ctatgtccag ttctacttcg atatgccggc ctgcatggac
     5881 acgctcacgg tcacgggtcg cggcgtctcg acctctcaat cgggcggcac cgcgccccgc
     5941 tactggctgt tcgtccgcaa ggatgagccg gtgacctaca ccatagacga ttacccggtc
     6001 gtcgtcgcac tcgacacggt cgtcgagccg gtggataaca tctatgaact caagaacttg
     6061 gaggcgggcg cccgctacta tctccatttc gtgaacgtcg cctcttccga aggggtcgtc
     6121 gcctacatct cggcgaacgc gacccggtcc agcgccgacc agtgcgtcga gaaccccgac
     6181 gatgtcgtgc ccgacgacga gacgaccgac gaactcgccg acgccgacac ggtcattgtc
     6241 cccgatgagg ccgacacgaa gaaaggatcg tcgggcggct gttcgctgat cttgttctga
     6301 tacgtcaact ctgacattcg gcgagtttcg ccgcgatctc atccagttgc agaagcagtt
     6361 gatcgccgtc ctcggcggtg aagtagagcg gatcaatgcc gagataggcc tcggtgcagc
     6421 ccgaggtgcc ggtcaggcag gcgttcggat cgggggcggt gccgccggcg cagaccagat
     6481 gattgagata gtcgggattc accgcgccgc cgaagccgac cacataggtc ttcacccccg
     6541 ccgcgagcag ttcctgcgcg ccgcaggaaa gcgtcgcctt ctcgcaggtc tccttgccgt
     6601 cggtcagcag gatgacgaac tgcttgcgct cggccggtga gggcgggtgg gcgacgaaga
     6661 agtcccgcgc cgagaggagc gcgcttttta tcggtgacga gcggcagagt ttcgcgcttt
     6721 ccatcgtgct gtttatcaat tcgccgttgt cgtaggcggg agggaccact acctctcctt
     6781 cgagacactg gacattgttc actttgcttc ccgcttctat ctcggcgatg gtgtagcacc
     6841 cttcgaccgt cttgggaaag aactcgaggc cgaaatagat accgctggcg aacttttcgc
     6901 cgatagcggc gatcgcctgt cgcgccatgt cccattttgt gacgccgtca acggcggtcc
     6961 gcatcgagag cgtccggtct atcgcgatga ggacctccgg ctcccagcag gtggaggtcg
     7021 cgtcgctgtc gggaacgaaa atatcgtcct cgtcgaaggt caccgcatcg tcaagcatgg
     7081 gaccgtcctc gaccacctcc ccttcatcgg ggagggcatc ctcctccggt gcgaacaggt
     7141 cggtatcgtc ggtctccccg acggcggggt cggtctccgg tagcgagttc tcgctctcgg
     7201 cagtgtcgac gaccacggta tcaggcgtga aactctcctc gtccaccggt tcatcgaccg
     7261 gcgcggagca ggagaccatg aacaagaaca ggaaagatcc gaacaccacg agctgttttc
     7321 tcatgacccc ctcccctcac tgaattcttt gaattatcta agattttccg ccgtaaagtc
     7381 aagagaaatc ttcgaagatg gcgttctgac cgataaggga gaacgcccct cccgttctcc
     7441 ctcacgcact gagacgattc ctgaaaaaaa gcgtggcact gtgggcttga agggcatcga
     7501 tggcgatgca agtgaacaac cgagatgatg gaggcggcca tgaaaaggat cgtagggttg
     7561 gcggcggtcg cgacggcggc ggccgtggtc ttcttcgctt gcaaggtggg cgaggtgacc
     7621 aaagaggcgc acgagaaccc cgacgagggg atcgtcaggt tcgatgacag cagtgtcgtg
     7681 caaaagatca aggagttgga aaagtcggcg ccggtcgaaa aaccggccga cgccgtaacg
     7741 accctcggcg acgaagggcg tcagaacgac aagaagagtt tcaagaaaga ggcgcagggt
     7801 tcgtcgcggc ggtcgatgga tggcgccgtt tttggctccg gcgggatcgg cacccgcggc
     7861 accggcgtag gcggtggcgg ctctgtcggg tatgggtacg gctatggttc gataacggtc
     7921 catgcttacg gccgggccgc cggcgcggcg gtcgtgcagt cgtacaccta cgacaccgaa
     7981 cagtatgaag ggaaagagga ggagggcttc cgctcggcgc ttcacgaccc gctctcgacc
     8041 ttctcgatcg atgtcgatac cgcgagttac gccaatgtgc ggcgctttct caccaccgat
     8101 cagttgccgc ccgtcgacgc ggtgcggatc gaagaactca tcaactactt cagttatcgt
     8161 tatcccgagc cgcaggggag cgaccccttc tcggtgacca ccgcgctcgc cgactgtccg
     8221 tgggcgccgg gtcatctcct cctgcaggtg gggctcaagg gaaaggagat cgcggccggg
     8281 aagatgccgc ccgcgaacct gaccttcctc atcgatgtgt cgggttcgat gaacgcgccg
     8341 aacaaactgc cgctcctgca gaaggccttc tctctgctcg tgaaggaact
//
LOCUS       DAOQUE010000061         8372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_9430_length_8372_cov_4.261392, whole genome shotgun sequence.
ACCESSION   DAOQUE010000061 DAOQUE010000000
VERSION     DAOQUE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9430_length_8372_cov_4.261392"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKH10_03045"
     CDS             <1..227
                     /locus_tag="PKH10_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27133.1"
                     /translation="RIGSDGSFLFTDRRFSPDRRYLAVMDGRIADLLLGTEDEEVSFE
                     SALPETLASRLTRAFPSHLSVPFAPLWRRD"
     gene            298..1695
                     /gene="ahcY"
                     /locus_tag="PKH10_03050"
     CDS             298..1695
                     /gene="ahcY"
                     /locus_tag="PKH10_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNT27134.1"
                     /translation="MKDHQVRDITLKDAGRKAIELAEIEMPGLMAVRERYSKAKPLKG
                     ARITGSLHMTVETAVLIETLKALGAEVRWASCNIFSTQDHAAAAIAASGTPVFAWKGE
                     TLEEYWECTLRALSFPKGQGPNLIVDDGGDATLMVHKGVEIEKNPKLLGKKTGNRELD
                     IVMCILAREHKKNPTKWRDMVKGLRGVSEETTTGVHRLYEMMKEGKLLFPAINVNDSV
                     TKSKFDNKYGCRESLADGIKRGTDVMIAGKMVVVCGYGDVGKGCAQSMRSYGARVIVT
                     EIDPICALQAAMEGYDVLTVEDVLPDADIFVTTTGNRDVITVDHMKKMKNMAIVCNIG
                     HFDNEIQVDALYALKGVRKINIKPQVDRIQFPDGHSILLLSEGRLVNLGNATGHPSFV
                     MSNSFTNQVLAQLDLWKTKYAIGVYTLPKKLDEEVARLHLAKLGVKLTKLTKSQAAYI
                     GVPVEGPFKSDHYRY"
     gene            1776..3542
                     /locus_tag="PKH10_03055"
     CDS             1776..3542
                     /locus_tag="PKH10_03055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNT27135.1"
                     /translation="MEMRWSLKELYPSFAAPAFKKDMERLAAEIVTLRRWTLTRLGKG
                     KNPVTTIEEYLKQRIALTTLNERLGVFANLSFAVNTSDDTAKTALEAIDRMLSETAED
                     RVKFAQWLAGVKNLDALAKRSKLIGEHLFHLREIQENTKHMLSEKEEVLFAKMRMTGS
                     TAWGSLQNTLTSTLMVPVKIGKKVEELPLPAARNLAYDPDKTVRKAAYEAELAAYAKV
                     DESSAACLNAIKGEVITTAALRGFDSPLAMTLHHSRMDKKTLDALLTAMRESLPQFRK
                     YLRKKAKLLGHKGGLPFYDLFAPVGKSDLRFTYYIAHQFIVRNFSSFSPELGAFADNA
                     FKKHWIDAEPRKGKVGGAFCYNIHPIGESRILSNFTGSFTNMTTLAHELGHAYHGHCL
                     KSESFLNSDYPMPLAETASIFCETIVTQAAIAAAPKRDVAAILENQLMGATQVIVDIY
                     SRYLFETALFAGRADSSVPVARLKTLMLNAQKEAYGDGLDKDTLHPYMWLCKPHYYDP
                     DHNFYNFPYAFGLLFAKGLYAEYLKRGDTFVKDYDRLLRETGKNDIATVAKLMRIDVR
                     KPDFWRGSLAIVGRDIDTFCAL"
     gene            3545..4057
                     /locus_tag="PKH10_03060"
     CDS             3545..4057
                     /locus_tag="PKH10_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNT27136.1"
                     /translation="MIEHLKKFIATGGYLGLMPVAPGTFGALWGVGLHLAGWCFLPKD
                     LLVPWLVAWLLLVSWQNHVLTPWAVEKWRGKARKEGDPGQFILDEIAGYLVVPIVFQV
                     DPFVAAIYGFLLFRVLDIIKIQPARWIDRNLHGSWGILLDDLVSGAYAGLLLHIAMLF
                     WSVERLLFWL"
     gene            4060..4338
                     /locus_tag="PKH10_03065"
     CDS             4060..4338
                     /locus_tag="PKH10_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNT27137.1"
                     /translation="MERKAYRYLVQGIVQGVGYRYFVQRNAEAIGLAGWVRNLPDGSV
                     EVYAVGTDEELARLEAALRHGPRHAEVTSLSVEKAPVDERPICFSVRS"
     gene            complement(4346..5944)
                     /locus_tag="PKH10_03070"
     CDS             complement(4346..5944)
                     /locus_tag="PKH10_03070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT27138.1"
                     /translation="MPERDISWVYYFVYPVLVAAAVYLVFIIFQYLRGLKDSPREIWL
                     VSISQVTEFVAWALMLSTLTLFLSSDVGLSDVMASNIVGGWNVSYTVLMFGVGALVDA
                     VGVKRVLVIGTVLAIGSRLIFAVSTNIYTLILFGFAVQAVSVAFLSPVITVAIKRYTK
                     SDTSAMGFAMFYTLMNVGFAIGGWLFDYIRNTLGEYGTTMVPVLGTMSTYRFIMLVAF
                     FLSIPAMIFISMLRDGVNLHDDGRVEITPPKKNEGAFFPALGKTIKDAFTDAYRIFID
                     VMKQPAFWKFMLLLTVLLGVNYVFFHFHYTFPKYGIRVLGEGAKVGSVYGMLNPVIVI
                     FFVPVIAWATRTWSPYLMITFGSFVSAFAVFLVTLPERVFMPLVDTVFGRLIWQVWLD
                     LDPALGPEKLSIYIGLTVFVGLFTVGEAIWSPRLMEYTARIAPEGQEASYMTLSMLPK
                     FISQPLVALMSGQLLANYVPVHDKIGPDGKAVLNEAGQVVQVVGDVSDHRMVWVWIAA
                     LAVTSPIGMLILRKWVNPKEPVKG"
     gene            6098..8011
                     /locus_tag="PKH10_03075"
     CDS             6098..8011
                     /locus_tag="PKH10_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT27139.1"
                     /translation="MGTNKKKRTEALVRSNADQMAQILDGLYQFIGLLDLDGTIRYLN
                     RAPVEAAGLPEDELLGLKFWDSFWWRGGDHELLKSDIRRAAAGEVIQRETPVLTKHGP
                     IVIEFNLYPIRDDDGRVVQMVAEGRNITERRKAELALRESERLLRQIIDLVPHFIFAK
                     DRDGRFILINRAVADNYGTTVEELTGKKDADFARSEEEVEHFRADDLAVIDSGTPKFI
                     PDEPITNAEGDIRSLTTVKIPFSFSGKKAVLGVSVDITERKRLWDEAQKVQRLEALGV
                     LAGGLAHDFNNLLSGIFGFIELARKVSKDPRTANYLDEALHSMARARGLTRQILTFAK
                     GGAPNCRTSFLQPLVLDTARFTLSGSLISFTADLSNDLPPCDHDPDQIGQVINNILLN
                     AQQAMPAGGTVEMAGRVVLVAEEDIPSLAPGRYIALSLSDHGIGIPAEILPRIFDPFF
                     TTKQKGSGLGLAVSHSIVRQHGGTITVRSEPGKGTTFTIYLPVSEKRMEPEVSPPAPA
                     DRTGSGRILLMDDEEMLLSLLSQMLERLGYATVCVRDGAVAVTAFTKAREAGTPFAAV
                     MLDLTVPGGMGGKEAAARIRHLDTDTPIFVFSGYADDPVMSSPLRYGFNDSLAKPFSF
                     EDLARLLSKHLMK"
     gene            8117..>8372
                     /locus_tag="PKH10_03080"
     CDS             8117..>8372
                     /locus_tag="PKH10_03080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT27140.1"
                     /translation="MGKVAFSTLSVIVPVYNEEENVAELHRRIVAALTGLPLDWEILY
                     VDDGSRDRSFDLLAEAAGDETRVKVLRFLRNYGQIVAIAAG"
BASE COUNT         1797 a         2644 c         2441 g         1490 t
ORIGIN      
        1 accggatcgg tagcgatggc tctttcctgt tcaccgatcg ccggttctct cccgaccgac
       61 gctatctcgc cgtgatggac gggcgtatcg ccgatcttct cctcggcacc gaggatgaag
      121 aggtctcctt cgaatcggcg cttcccgaaa cactcgcctc ccgtctgacc cgcgcgttcc
      181 cctcgcatct ttcagtcccc ttcgcgcccc tctggaggcg cgactgacct ttttgcttgc
      241 aatcgggccc cgtcccgtta tgatagcttc cgcattcata ccgatcgtgg aggtaacatg
      301 aaagaccatc aggtacggga catcacgctc aaggacgcag gccgcaaagc gatagaactc
      361 gccgagatcg agatgccggg actcatggcg gtgcgcgaac gctactcgaa ggcaaaacct
      421 ctcaaggggg cgcgcatcac cgggtcgctc cacatgacgg tcgagacggc ggtgctcatc
      481 gagacgctca aggcgctcgg ggctgaggta cgctgggcct cgtgcaatat cttctcgacg
      541 caggaccacg cggcggcggc gatagcggcc tccggtacgc cggtcttcgc atggaaggga
      601 gagacgctcg aagagtactg ggaatgcacg ctgcgggcgc tctcgttccc gaaaggacag
      661 gggccaaacc tcatcgtcga cgacggcggc gacgcgacgc tcatggtaca caaaggggtc
      721 gagatagaaa agaacccgaa actgctcggc aagaagacgg gcaaccgcga actcgacatc
      781 gtcatgtgca tcctagcccg tgaacacaaa aagaacccga ccaagtggcg cgacatggtc
      841 aaagggctgc gcggcgtcag cgaagagacc accaccggcg tccaccggct ctacgagatg
      901 atgaaggaag gaaaactgct ctttcccgcg atcaatgtca acgactcggt caccaagtcg
      961 aagttcgaca acaaatacgg ctgtcgtgaa tcgctcgccg acggcatcaa gcgcggcacc
     1021 gacgtgatga tcgcgggcaa gatggtggtg gtgtgcggct acggcgatgt cggcaagggg
     1081 tgcgcccagt cgatgcgctc ctacggcgcc cgcgtgatcg ttacggagat agaccctatc
     1141 tgcgctctgc aggcggcgat ggagggctac gatgtcctca ccgtcgagga tgtcctgccc
     1201 gatgcggaca tattcgtcac gacgaccggc aaccgcgatg tcatcaccgt cgaccacatg
     1261 aaaaagatga agaatatggc catcgtctgc aacatcggcc attttgacaa cgagatacag
     1321 gtcgacgcgc tttacgcact caagggtgtc cggaagatca acatcaagcc gcaggtcgac
     1381 cgtattcagt tccccgacgg tcactcgata cttctgctgt ccgaagggcg gctcgtaaat
     1441 ctcggcaacg cgacggggca tccctcgttc gtcatgtcga attccttcac caatcaggta
     1501 ctcgcccaac tcgatctttg gaagacgaag tacgcgatag gggtctacac gcttcccaag
     1561 aaactcgacg aggaggtggc gcggctccat ctggcgaaac tcggcgtgaa actgacgaaa
     1621 ctcaccaagt cacaggcggc ctacatcggc gtcccggtcg aaggaccctt caaaagcgac
     1681 cactaccgtt actgaaaccc tttttctgtt ttcttgcacc attccccgtc tcccgctata
     1741 ctgcgtcgtt atgaactcac acggggggga tccctatgga gatgcgctgg agcttgaaag
     1801 agttgtaccc gtcgttcgcc gcacccgcct tcaagaagga catggagcga ctcgccgccg
     1861 agatcgtcac cctgcgccgc tggaccttaa cgcggctcgg caagggcaag aacccggtga
     1921 cgaccatcga agaatacctg aaacagcgca tcgcgctgac gacgcttaat gagcggctcg
     1981 gcgtcttcgc caacctctcg ttcgccgtca acacctccga cgacaccgcc aagacggcgc
     2041 tcgaagcgat cgaccggatg ctctccgaga cggccgagga ccgcgtgaag ttcgcccagt
     2101 ggctcgccgg ggtgaagaac ctcgacgcgc tggcgaagag atcaaaactc atcggcgaac
     2161 atctcttcca cctgcgcgag atacaagaga acaccaagca catgctctcg gagaaggagg
     2221 aggtgctctt cgccaagatg cggatgaccg gctcgaccgc gtggggctcg ctccagaaca
     2281 ccctcacctc gaccctcatg gtgccggtga agatagggaa aaaggtggaa gagttgccac
     2341 tcccggcggc ccgcaacctc gcctacgacc ccgacaagac ggtgcgcaaa gcggcctacg
     2401 aggccgaact ggcggcctac gcgaaggtcg acgaatcgtc ggccgcgtgc ctgaacgcga
     2461 taaagggcga ggtcatcacc accgccgccc tgcgcgggtt cgacagtccg ctcgccatga
     2521 cgctccacca ttcgcggatg gataaaaaga cgctcgacgc gctcctcacg gcgatgcgcg
     2581 aaagtctgcc gcagttccgt aaatatctcc gcaaaaaggc aaaactgctc ggccataagg
     2641 gcggcctgcc gttctacgat ctcttcgcgc cggtgggaaa gagcgacctg cgtttcacct
     2701 actacatcgc ccaccagttc atcgtgcgca acttctcttc cttcagcccg gaactgggcg
     2761 cgttcgccga caacgccttc aagaaacact ggatagacgc cgaaccgcga aaaggcaagg
     2821 tgggcggcgc cttctgctac aacatccatc ccatcggcga gagccggatc ctgtcgaact
     2881 tcaccggcag tttcaccaat atgaccacgc tcgcccacga actggggcac gcctaccacg
     2941 gccactgcct gaagtccgaa tcgttcctca acagcgacta cccgatgcca ctggccgaga
     3001 ccgcgtcgat attctgcgag accatcgtca cgcaggcggc gatcgcggcg gcgccgaagc
     3061 gcgacgttgc cgcgatactc gagaaccaac tcatgggggc gacgcaggtc atcgtcgaca
     3121 tttatagccg ctatcttttc gagacggcgc ttttcgcggg ccgcgccgat tcgtcggtac
     3181 cggtcgcgcg gctcaagacc ttgatgctca acgcccagaa ggaggcctac ggcgacggac
     3241 tcgacaagga tacgctccat ccctacatgt ggctgtgcaa accgcactac tacgaccccg
     3301 accacaactt ctacaacttc ccctacgcct tcggactcct cttcgccaag gggctctacg
     3361 ccgaatacct gaagcgcggc gacaccttcg tgaaggatta cgaccggctc ctgcgcgaga
     3421 cggggaagaa cgacatcgcg acggtcgcca aactgatgag gatcgatgtc cgcaagcccg
     3481 atttctggcg cgggtcgctc gctatcgtcg gccgcgacat cgataccttc tgcgcgctct
     3541 agtcatgatc gaacacctga agaagttcat cgccaccggc ggctatctcg gccttatgcc
     3601 tgtcgcgccg gggaccttcg gagcgctctg gggcgttgga ctccatctcg cggggtggtg
     3661 cttcctgccg aaggacctcc tcgtcccgtg gctcgtcgca tggctcctcc tcgtctcgtg
     3721 gcagaatcat gtcctcacgc cgtgggctgt cgagaaatgg cgaggcaagg cgcgcaagga
     3781 aggcgacccg gggcagttca tcctcgacga gatcgccgga tatctggtgg tccccatcgt
     3841 ctttcaggtc gacccgttcg tcgcggccat ctacggcttt ttgttgttcc gcgtcctcga
     3901 catcatcaag atacagccgg cccgctggat agaccgcaac ctgcacgggt catggggaat
     3961 actcctcgac gatctggtga gcggcgccta tgccgggcta ttgctgcaca tcgccatgct
     4021 tttctggagc gtcgaacgac tgttgttctg gctatgatca tggaacggaa agcgtaccgg
     4081 tatctcgtcc aaggcatcgt acaaggggtg ggctaccgtt atttcgtaca gcggaacgca
     4141 gaggccatcg gtcttgccgg atgggttcgc aatctgcccg acggctcggt cgaggtgtac
     4201 gcggtcggca ccgacgagga attggcgcga ttagaagcgg cgctacggca cgggccgcgt
     4261 catgcggagg taacatccct ctctgtcgaa aaggcaccgg tcgacgagcg accgatctgt
     4321 ttttcagtgc gatcctgaag cagggtcacc ccttcaccgg ctccttgggg ttcacccatt
     4381 tcctgagtat cagcatcccg atgggcgagg tgacggcgag cgccgctatc cagacccaga
     4441 ccatccggtg gtcggacaca tcgcccacca cctgcaccac ctgccccgcc tcgttgagca
     4501 ccgccttgcc gtcggggccg atcttgtcat gtaccggcac atagttcgcg agcagttggc
     4561 ctgacatgag ggcaacgagc ggctgggaga tgaatttggg aagcatcgag agcgtcatgt
     4621 aactcgcttc ctgaccctcg ggagcgatgc gggcggtgta ttccatcagg cgcggcgacc
     4681 agatcgcctc gccgacggtg aatagcccga cgaagacagt aagcccgatg tagatggaga
     4741 gtttctcggg accgagcgcc gggtcgaggt cgagccatac ctgccagatg agccgaccga
     4801 acacggtgtc caccagcggc atgaagaccc gctcggggag cgtcacgagg aagacggcga
     4861 aggccgacac gaacgacccg aaagtgatca taaggtacgg gctccaggtc cgggtcgccc
     4921 acgcgatgac cggcacgaag aatatcacga tgaccgggtt gagcatcccg tagaccgagc
     4981 cgaccttcgc cccttcgccc aacacgcgga tgccgtattt cgggaaagtg taatggaagt
     5041 ggaagaagac atagttgaca ccgagcagta ccgtgaggag caacatgaac ttccagaaag
     5101 cgggctgttt catgacatcg atgaagatgc ggtaggcatc ggtgaaagca tccttgatcg
     5161 tcttaccgag cgcggggaaa aaggcccctt cgttcttttt gggcggcgtt atctcgacgc
     5221 gcccgtcatc gtgcaggttg accccgtcgc ggagcatcga gatgaagatc atcgccggta
     5281 tcgacaggaa gaaggcgacg agcatgatga aacggtaggt cgacatcgta ccgagcaccg
     5341 gcaccatcgt cgtgccgtat tcgcccagcg tattgcggat gtagtcgaac aaccacccgc
     5401 cgatcgcgaa gccgacattc atgagcgtgt agaacatcgc gaagcccatc gccgaggtgt
     5461 cagacttggt gtagcgcttg atcgcgacgg tgatgacagg tgacagaaag gcgaccgaca
     5521 ccgcctgcac cgcgaaaccg aacaggatga gcgtgtagat gttggtcgag acggcgaaga
     5581 taagccgcga accgatggca agcaccgtcc cgatgacgag cacccgcttc acgccgaccg
     5641 cgtccacaag cgcgccgacg ccgaacatga gcaccgtgta ggacacattc cagccgccga
     5701 cgatgttgct cgccatgaca tccgacaggc cgacatcgct cgacaggaaa agcgtgagcg
     5761 tcgagagcat gagcgcccat gcgacgaact cggtcacctg cgatatcgag acgagccata
     5821 tctcgcgggg cgaatccttc aggcctcgaa gatactggaa gatgatgaag acgagataca
     5881 cggcggcggc gacaagcacc ggatagacga aataataaac ccaactgata tcccgttccg
     5941 gcatgacgcg ctccgtaccc cgttagtggt ggtcgatgct agcagaaagg aaaaggggtg
     6001 tcaaatcggg cggcgacagt ttcccggaaa aactttacga cacgatggat ccccgctaga
     6061 atgccctcgc attcgtcgtg aggggggggg gaacaccatg ggcaccaaca agaagaaacg
     6121 gaccgaggcg ctcgtccgat cgaatgccga ccagatggcg cagatactcg acgggctcta
     6181 ccagttcatc ggtctgctcg acctcgacgg caccatccgc tacctcaacc gcgcgccggt
     6241 cgaggcggct gggctacccg aggacgaact gctcggcctc aagttctggg acagtttctg
     6301 gtggcgcggc ggcgatcacg aactactgaa aagcgatatc cggcgcgccg cagcgggcga
     6361 ggtcatccag cgcgagaccc cagtgctcac caagcacggc cccatcgtca tcgaattcaa
     6421 tctctatccg atccgggatg atgacgggag ggtggtacag atggtcgccg aagggcgcaa
     6481 catcactgag cggcgcaagg ccgaactggc cctgcgcgag agcgagcggc tcctacggca
     6541 gatcatcgat cttgttcccc acttcatatt cgccaaggac cgcgacggtc gattcatcct
     6601 catcaaccgc gcggtcgccg acaactacgg taccaccgtc gaggaactca ccgggaaaaa
     6661 ggacgccgac ttcgcgcgat cagaggagga ggtcgagcac ttccgggccg acgatctcgc
     6721 ggtcatcgac tcgggcaccc ccaagttcat ccccgacgag ccgataacga acgcggaagg
     6781 ggacatccgt tcactcacga cggtcaagat ccccttctcc ttctccggga agaaggcggt
     6841 gcttggcgtt tcggtcgaca tcaccgaaag gaaacggctc tgggatgagg cgcagaaggt
     6901 ccagcgactc gaggcgctcg gcgttcttgc gggagggctt gcgcacgact tcaacaacct
     6961 cctttcgggc atcttcggtt tcatcgaact ggcgcgcaag gtatcgaagg acccgcgcac
     7021 ggccaactac ctcgacgagg cactccactc gatggcgcgc gcgcgcggcc tcacgaggca
     7081 gatactcacc tttgccaagg gaggtgcccc caactgccga acctcgttcc tccaaccgct
     7141 cgtccttgat acggcacgat tcaccctctc ggggtcactg atctccttta ccgccgacct
     7201 ctccaacgac ctgcccccgt gcgaccacga ccccgaccag atcgggcagg tcatcaacaa
     7261 catcctgctc aatgctcaac aggcgatgcc cgccggcggc acggtcgaga tggcgggcag
     7321 ggtcgtcctc gtcgcggaag aggacatccc gtcgctcgcg ccgggtcgct acatcgccct
     7381 ttccctaagc gatcacggca tcggcatacc ggccgagata ctgcctcgca tattcgaccc
     7441 gttcttcacg accaaacaga aagggagcgg gctcggactc gccgtgtccc actcgatagt
     7501 gcgccagcat ggcggcacga tcaccgtgcg atccgagccg ggcaagggaa cgaccttcac
     7561 catctatctg ccggtctcgg agaaacgaat ggagcccgag gtatcgcctc ctgcacccgc
     7621 cgaccgcacc ggttcgggca ggatactcct catggacgac gaagagatgc tcctgtcgct
     7681 cctttcccag atgttggaac ggcttggata tgcgacggtc tgcgtcaggg acggcgcggt
     7741 cgccgttacc gctttcacaa aggcgcgcga agccggtacc cccttcgccg ccgtgatgct
     7801 cgatcttacg gtgccgggcg gcatgggcgg caaagaggcg gcggcccgga tacggcacct
     7861 cgataccgac accccgatct tcgtcttctc aggatacgcc gatgatcccg ttatgtcctc
     7921 ccccctccga tacgggttca acgacagtct ggccaagccg ttctctttcg aggatctcgc
     7981 gcgactcctc tcaaaacatc tgatgaaata acctcccgcc cctctttctg cctctcataa
     8041 gttatgttgc ttttcacccg cccctcgcat atcatacgac cgtatgtcgg gaaccccatc
     8101 gaaaggcggt aaacgcatgg gaaaggtcgc tttttcgacc ctgtcggtca tcgtgccggt
     8161 ctacaacgag gaggagaatg tcgccgaact gcaccggcgc atcgtcgcgg cgctcacggg
     8221 actcccgctt gattgggaga tactctatgt ggacgacggt tcgcgcgacc gcagtttcga
     8281 tctacttgct gaagcggcgg gcgatgaaac acgcgtgaag gtgctccgct tcctccgcaa
     8341 ctacggccag attgtcgcca tcgccgccgg ta
//
LOCUS       DAOQUE010000062         8359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_9453_length_8359_cov_4.164379, whole genome shotgun sequence.
ACCESSION   DAOQUE010000062 DAOQUE010000000
VERSION     DAOQUE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9453_length_8359_cov_4.164379"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="PKH10_03085"
     CDS             complement(<1..1280)
                     /locus_tag="PKH10_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27141.1"
                     /translation="MKRSILLVAILSVFTLHAQNYDVGNIAVVHGDASIASGLIGVLE
                     QAAGQQFYMTHGDLFDFLIIYTTFTPQINMQQGVPIVDAAQGIGRENVGFLYGPASKW
                     GSAGRLKGGVRMIHVDKYPDDPDANMAFPLAGLTSVELLAHEFSHYWLSAMDFKKEGA
                     ADPHTGLRGWEDGANQHWNHYFSSGPSVMYGSHIVDNGDGSFTFDPATPRKYGPLDQY
                     VMGLRTPEEVGSLFFLCSYADIASCLEGSPSLPTPHTGSIDTISNMYKHEVTMDDILR
                     AMGPRVPAAADAQHDFNAAFIIINLPGVEPFPNQLTRLETLRVRFQEWFSWATDGRGT
                     LCTELDGDCGNVEPTDDDVAPDDTVTDDIVTDTELPDDTVIDDALSDDVPVNDASQID
                     DELPDTAVIDNGGSSDDIVDTTGAADEVIVGEDEA"
     gene            complement(1292..2620)
                     /locus_tag="PKH10_03090"
     CDS             complement(1292..2620)
                     /locus_tag="PKH10_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27142.1"
                     /translation="MKKIVFACLLSAFFGLYAQNYDVGNIAVVHGGPDVAGGLIGVQE
                     KTAGLQFYMTHADIYDFMVVFTTYTPQMNMQQGLAIQYTVQGINREGAWSPYGPASAW
                     GSAGRLIGGTRMCHIDQYPDDPDVPISNNIISPFAGMSTVELLAHEFSHYWLMAIDFK
                     KEGMAENHTGLRGWEDGANQHWNSYAGSGPSVMYGSHVVDNGGGSFTQVFSLPRKYGH
                     LDQYVMGLRAPEEVDPILFICSSSDIEQCREGSPAIPAAKTSPDKTYNSGDMYLHTVT
                     IEDVIRAMGPRIPAAADAPKHWNVAFVIINLPGQDPFPNQITRLDTLRVRFQEWFTWA
                     TDGRATICTELDGDCGNVEPTDDDAVSDESVTDDIVTDTELPDDTVTDDTVTDDQPIV
                     DDVAPDTAVTDDAVADNGETPDEVVDESGNDGVVVGDEGDEGGCGCSLMF"
     gene            complement(2846..4489)
                     /locus_tag="PKH10_03095"
     CDS             complement(2846..4489)
                     /locus_tag="PKH10_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27143.1"
                     /translation="MAKVLKVRCGPGTVLTTKIKKALVPERDWSSSASVRHEKIIVMA
                     SVIFVAWALSFIWRTSFIAVDDCRYFCLFDDAMISLRNALNFVRGEGLVWNPGEYVEG
                     YTNTFMVLIMALGIFLFGSQGGVLSTQLLAVPTILLLSYCSREISFFLTKEKDEQTRV
                     LVGRSVFIMILCCYPLLYLSLMGMETGFLSLFFLFSILSLLRYMEKGLSRDIIMASFS
                     LSAAFLTRNDSLILAAPFIGYAFLRQRKLLPWVFWGTILLGSVVGLSLFRLTYYGEIL
                     PNTYFLKLGGIPLWARIDNGVRYTLLSLAISAPLFMAVLYDWLCYDRTWIKTPFFATI
                     ASAFLYQIYIGGDAWRLWRMILPVVPLLFMLFALSMISLKRYFFSLRYVLVPRVVFPI
                     VMLLVFNVNYLPEIFFLDSPFHVETNRHHVNIAIAISEVTSKNSSVGVISAGTIPYFS
                     DRHAIDFLGKSDRYIARLSPYLDKTPSSRIMRYTPGHNKYDLGYSIFKLRPTYIQQAE
                     YVFQDYTDKAQGVYVLKHYKGVPLLLLRNSPDVFWEKLN"
     gene            complement(4617..>6907)
                     /locus_tag="PKH10_03100"
     CDS             complement(4617..>6907)
                     /locus_tag="PKH10_03100"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNT27144.1"
                     /translation="SAAETYYKQESRTVCYLSAEFLLGPHLGNNLINLGLWDEVKAAV
                     AALGQDLETLLEQEEEPGLGNGGLGRLAACYMDSLATLNIPAIGYGIRYEFGIFDQEI
                     RDGWQTEVTDKWLRYGNPWEIARPEIAFDVKFGGHTEMYRDEADALRVRWVPSHVVKG
                     VAYDTMIQGYKTSTASLLRLFKAEAVESFDFSSFNVGDYYGAVNEKVSSENLTKVLYP
                     NDEPLAGKQLRLKQQYLFVSCALQDMIRIYLQRERTLDRFHEKYAIQLNDTHPSIGVA
                     ELMRLLVDEYWYPWEEAWEITRKTFCYTNHTLLPEALEKWPVPLFRDLLPRHLEIIYE
                     INARFLAGVRERYPNDDEMLRRLSLIDETGDRYVRMANLACVGSQAINGVAALHSELL
                     KRDVLYDFNRIMPEKFSNKTNGVTPRRFVVLANPGLAKLISGRIGEGWIRDLHELKKL
                     EEFAGDGAFRAAWREVKAANKRRLAMLLHERTGVEVDPESLYDVQVKRIHEYKRQHLN
                     LLHAVTLYNRIRRGETAGMTPRTVIIGGKAAPGYFMAKRIVKLVSSIAETVNNDPAAK
                     GLLTVVFFPDFNVKNAQFIYPAADLSEQISTAGKEASGTGNMKFSMNGALTIGTLDGA
                     NVEIREEVGAENFFLFGLQTEEVGELKRNGYRPQEYIENDDELREALDAIGGGLFSNG
                     DTELFRPLVDNLRYSDPYLLCADYRSYIAAQEAVGATYRDTGRWTRMAILNVARMGKF
                     SSDRAIREYCDDIWKVKPVLVD"
     assembly_gap    6908..6917
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7178..>8116
                     /locus_tag="PKH10_03105"
     CDS             7178..>8116
                     /locus_tag="PKH10_03105"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HNT27145.1"
                     /translation="MHSPRLATIAIVAVSVLFLATLATLSVFVHPSADDFCAAVRVRD
                     LGFWPAQSEIYRNWSGRYSSSLLIFFLRWTDSLPLYRLAHALLLLFSVFSCFFLFERL
                     LPVGTPRRVPLTGAGIFSFLFFAGTPSLVEAAYWLGGAEMYHFSTSLTLLLVGASLSP
                     RRILHERVDHFLLALCILIVAGSNEISMLLLIIILAAATLFRSLRAGRIDRRSAFLLG
                     LAALFSLGVILAPGNAARSLSYTHHHDILFSTGATVALLSALAPMWLMSPLLWICSLC
                     YLWWRRTGALSPSLPAHRRMPTIILFLLLLAGTVFPS"
     assembly_gap    8117..8126
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8127..>8359
                     /locus_tag="PKH10_03110"
     CDS             <8127..>8359
                     /locus_tag="PKH10_03110"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27146.1"
                     /translation="NITYAVFLLFLLYLLETVMLPWITTKFPTHTRFYGRAIFVALAF
                     FVLYSVVIASIPRQKEWSRDIEQYLAEPSRAVA"
BASE COUNT         1766 a         2486 c         2265 g         1822 t
ORIGIN      
        1 gcctcatcct cgccaacgat cacctcatcg gcggcgccgg tcgtgtcgac gatgtcgtcg
       61 gatgatccgc cgttatcgat aacagcggtg tcgggcaatt cgtcgtctat ctgggaggcg
      121 tcgttcaccg gcacatcgtc cgacagggcg tcgtcgatca ccgtgtcgtc cggcagttcg
      181 gtgtcggtca cgatgtcgtc ggtgacggta tcatcaggcg ccacatcgtc gtcggtcggc
      241 tcgacattcc cgcagtcacc gtcaagttcg gtgcacagtg tcccgcgccc gtcggtcgcc
      301 caactgaacc attcctggaa gcggacccgg agcgtttcaa gtcgcgtcag ctggttgggg
      361 aagggctcca cgccgggcag atttatgatg atgaaggcgg cgttaaaatc gtgctgggca
      421 tcggccgccg ccggtacgcg cggccccatc gcccggagga tgtcgtccat cgtcacctcg
      481 tgcttataca tattgctgat ggtgtcgatg gagccggtgt gcggcgtcgg aagagagggg
      541 ctcccctcca gacaactggc gatatcggca taactgcaca ggaaaaagag cgagccgacc
      601 tcctcggggg tgcggagccc catgacatac tggtcgagcg ggccatattt tcgaggtgtt
      661 gccggatcga aggtgaacga cccgtcgccg ttgtccacga tatgactgcc gtacatgacc
      721 gacggacccg aagagaaata atggttccag tgctgattcg cgccatcctc ccagccgcgc
      781 aggccggtgt ggggatcggc ggcgccctct ttcttgaaat ccatggccga aagccagtag
      841 tggctgaact catgcgcgag cagttccacc gaggtgagcc cggcgagcgg gaaggccatg
      901 ttggcgtcgg gatcatcggg atacttgtcg acatgtatca tccggacccc tcccttcagc
      961 cgaccggcgc tcccccactt cgaggcggga ccatacagga agccgacatt ctcgcgcccg
     1021 atcccctgcg ccgcatcgac gatgggaacc ccctgctgca tgtttatctg cggggtgaag
     1081 gtggtgtaga tgatgaggaa atcgaacagg tcgccgtggg tcatgtagaa ctgctgaccc
     1141 gccgcctgct ccaacactcc gatgagcccg ctggcgatac tcgcatcgcc gtggaccacc
     1201 gcgatgttac ccacatcgta gttctgggca tgaagcgtga acaccgacaa gatcgcgacc
     1261 agcagtatgg accttttcat ctatctcctc cctagaacat aagtgaacaa ccgcaccccc
     1321 cctcatcgcc ttcgtcgccg accaccacgc cgtcgttccc actttcatcc accacctcgt
     1381 cgggcgtctc gccgttgtcg gcgaccgcgt catcggtcac ggcggtgtcg ggagcaacat
     1441 catcaacgat cggctggtcg tcggtcaccg tatcatcggt cactgtatca tcgggcagtt
     1501 cggtatcggt aactatgtcg tcggtcacgc tttcgtcgga caccgcatca tcgtcggtcg
     1561 gttcgacatt gccgcagtcg ccgtcgagtt cggtgcagat cgtggcgcga ccgtcggtcg
     1621 cccaagtgaa ccactcctga aagcggacgc ggagcgtatc gagccgtgtg atctgattcg
     1681 ggaacgggtc ctgacccggc aggttgatga tgacgaacgc gacattccag tgcttcggcg
     1741 cgtcggccgc cgccgggatg cgcggcccca tcgcgcggat gacatcctca atggtcaccg
     1801 tatggagata catgtcgccg ctattatagg tcttatcggg agaggtcttg gccgccggta
     1861 tggcggggct tccttcacgg cactgttcga tgtcggagga ggaacagatg aagagtatcg
     1921 gatcgacctc ttcgggagcg cgcaagccca tgacatactg gtcgagatga ccgtatttgc
     1981 gcggaagaga gaacacctgc gtgaaggatc cgccgccgtt atcgacgaca tgactgccgt
     2041 acatgaccga cgggcccgaa ccggcgtaac tgttccaatg ctgattcgcg ccgtcctccc
     2101 aaccgcgcag gccggtgtgg ttctcggcca tcccctcttt cttgaaatct atcgccatga
     2161 gccagtagtg gctgaactcg tgcgcaagca gctcgaccgt actcatcccg gcgaagggcg
     2221 agatgatatt gttcgatatc ggcacatcgg ggtcgtcggg atactggtcg atatgacaca
     2281 tccgggtacc gccgatgagc cgaccggcgc tcccccacgc ggaggcggga ccgtacggcg
     2341 accatgcccc ttcgcggttt atcccctgca ccgtgtactg gatggcgagt ccctgctgca
     2401 tgttcatctg cggcgtatag gtcgtgaaga ccaccatgaa gtcgtagatg tcggcgtggg
     2461 tcatatagaa ttgcaggccc gccgtctttt cctgcacgcc gatgagtccg ccggcgacat
     2521 cgggcccacc gtggacgacc gcgatgttac cgacatcgta attctgggca tacaggccga
     2581 aaaaggccga aagaaggcac gcaaaaacga tctttttcat atcgaactcc gtgactaagg
     2641 ttccaacacg gtaggcatga tacctaaaat gaaaggaaaa agcaaaagga aaaaacgacc
     2701 aatgcctgta tgtgttcttc actatttccc atgtgcgacg gaaggaggcg atgtatgcga
     2761 tggcttcgtg gagtgatcta cggtcagcct catagatctc acaccgcccg cctcatgtct
     2821 cacgctcata ccgccccccc cctacctagt ttaatttctc ccaaaaaaca tccggggaat
     2881 ttctcaacag caaaagaggt actcctttgt aatgctttag gacatataca ccctgtgcct
     2941 tgtccgtgta atcttgaaaa acatactctg cctgctggat ataggtcgga cgcagtttaa
     3001 aaatagaata acccaggtca tacttgttat gaccgggagt atatctcatg attctgctgg
     3061 aaggggtctt atcaaggtaa ggggacaatc gagcgatgta tcgatctgat tttccgagaa
     3121 agtctattgc gtggcgatca gagaagtaag gaatcgttcc cgccgagata acacccacag
     3181 aagaattctt tgaagtaacc tcactaattg caattgcgat gttgacatga tgccgatttg
     3241 tttcaacatg aaaaggagag tcaagaaaga agatctccgg cagataattg acattaaaca
     3301 caagcaacat gactatagga aaaacaacac ggggaaccag aacatatcgc aaagaaaaga
     3361 aatacctctt caaagagatc atcgaaagag caaagagcat aaaaagaagc ggcaccaccg
     3421 gcaagatcat tcgccaaagt cgccaagcgt caccaccgat ataaatctgg taaagaaaag
     3481 cacttgctat tgtcgcaaaa aaaggggttt ttatccatgt tctatcatag caaagccagt
     3541 catataatac agccataaaa agaggagcac ttattgccaa ggacaagagg gtgtagcgaa
     3601 ctccattgtc gattcttgcc cataaaggaa taccgccgag ctttagaaaa taagtgttgg
     3661 ggaggatttc accgtagtaa gtcaacctaa aaagagacaa tcccaccaca ctgcccaaga
     3721 gaattgtccc ccaaaatacc caagggagaa gttttcgttg gcggagaaaa gcgtacccta
     3781 tgaaaggggc cgcaagaatc aaagaatcat tcctggtcag gaatgcggca ctcagagaga
     3841 atgatgccat gatgatgtcc cggctaagtc ctttttccat gtagcgaagg agagaaagga
     3901 tggaaaaaag aaaaaacaac gaaagaaaac cggtttccat tcccatcaag gataagtaca
     3961 aaagagggta gcaacacaaa atcattatga aaacagatct accgacaagg actcttgttt
     4021 gctcgtcttt ttcttttgtc agaaaaaagg atatctcccg agaacaataa gaaagtagaa
     4081 gtattgtcgg cacggcaagc agttgtgtgg acaagactcc tccttgggat ccaaaaagaa
     4141 aaattcccaa ggccatgata aggaccatga aggtgttagt gtatccctcg acatattcgc
     4201 cggggttcca gacaaggcct tcgccacgaa caaaatttaa tgcatttcta agggaaatca
     4261 ttgcgtcatc aaaaagacaa aagtaccggc aatcatccac agcgataaaa gaagttctcc
     4321 agataaaaga cagagcccac gcgacaaaaa taacagatgc catcacaatg attttttcat
     4381 gtcgcacgga ggcagaagac gaccaatctc tttccggcac aagggctttc ttgatttttg
     4441 ttgttaaaac cgtacctggc ccacacctga cttttaatac tttagccatc gagcacattc
     4501 tctaaattct atctatctgc atccctgatt cctgagctcc ccatttctct tcgctcccct
     4561 gattaaaata cggctctgtc cctaatcccc accgcgatca ttttttcagc cgaaagttaa
     4621 tccacgagga ccggcttgac cttccagata tcgtcgcagt actcgcggat ggcgcggtcg
     4681 gaactgaact tacccatccg ggcgacattg agtatcgcca tgcgggtcca gcgaccggtg
     4741 tcgcggtagg tcgcccccac cgcctcctga gcggcgatgt aggagcggta gtcggcgcag
     4801 aggagatacg ggtcgctgta gcgcaggttg tcgaccagcg gccggaacag ttcggtgtcg
     4861 ccgttcgaga aaagcccgcc gccgatcgcg tcgagcgcct cccgcagttc gtcgtcattt
     4921 tcgatgtatt cctgcggccg gtagccgttc cgcttgagtt cccccacctc ctcggtctgg
     4981 aggccgaaca ggaagaagtt ctcggccccc acctcctcgc gtatctcgac attggcgccg
     5041 tcgagcgtcc cgatggtcag cgcgccgttc atagagaact tcatgttgcc ggtccccgac
     5101 gcctccttgc ccgccgtcga tatctgttcc gacaggtcgg ccgccggata gatgaactgc
     5161 gcgttcttga cattgaagtc agggaagaag accacggtga gcaacccctt cgccgccgga
     5221 tcgttgttga ccgtttcggc gatggaactg acgagtttga cgatccgttt ggccatgaag
     5281 tagccggggg ccgccttgcc gccgatgatg acggtgcgcg gcgtcatgcc ggccgtctcg
     5341 ccgcgccgga tgcggttgta gagcgtcacg gcatgcagga gattcaggtg ctggcgtttg
     5401 tattcgtgga tccgcttgac ctgcacatcg tagagactct cgggatccac ctcgacaccg
     5461 gtccgttcat gcaggagcat cgcgagtcgc cgtttgttgg ccgccttcac ctcgcgccac
     5521 gcggcccgga aagcgccgtc gcctgcgaac tcctcgagtt tcttcaattc gtggagatca
     5581 cgtatccagc cctcccctat ccggccgctg atgagtttcg cgagccccgg gttggcgagg
     5641 accacgaacc ggcgcggcgt cacgccgttg gtcttgttcg agaacttttc gggcatgatg
     5701 cgattgaaat cgtacagcac atcgcgcttc agcagttcgc tgtggagcgc cgccacgccg
     5761 ttgatggcct gcgacccgac acaggcgagg ttcgccatcc ggacataccg gtcgcccgtt
     5821 tcgtcaatga gcgacaggcg ccgcagcatc tcgtcgtcgt tcgggtatcg ctcccgcacc
     5881 ccggcgagga aacgggcgtt tatctcgtag atgatctcga gatggcgcgg caggagatcg
     5941 cggaacagcg gcaccggcca tttctcgagc gcctcgggaa gcagggtgtg attggtgtag
     6001 cagaaggtct tgcgggttat ctcccacgcc tcttcccatg gataccaata ctcatccacc
     6061 agaagacgca tcaactcggc caccccgatg gagggatggg tgtcattcag ttgtatcgcg
     6121 tatttctcgt ggaagcgatc aagcgtccgt tcgcgctgga gatatattcg tatcatgtcc
     6181 tgcagggcgc aggagacgaa aagatactgc tgtttgaggc ggagttgctt gcccgccagc
     6241 ggctcatcgt tcgggtagag caccttggtg aggttctcgg aggagacctt ttcgttcacc
     6301 gcgccatagt agtcgccgac attgaacgaa ctgaaatcga acgactcgac cgcctcggcc
     6361 ttgaacaggc gcaggagact cgccgtgctc gtcttgtagc cctgtatcat cgtatcgtag
     6421 gcgacccctt tcaccacatg gctcggcacc cagcggaccc gcagggcgtc ggcctcgtcg
     6481 cggtacatct cggtatgccc gccgaacttg acatcgaacg ctatctcggg ccgcgcaatc
     6541 tcccacgggt tgccgtaccg gagccacttg tcggtcacct cggtctgcca accatcgcgt
     6601 atctcctgat cgaagatacc gaattcgtag cggatgccgt acccgatggc ggggatgttg
     6661 agcgtggcca acgaatccat gtagcaggcc gccaagcgcc cgagcccgcc gttgccgagc
     6721 cccggttcct cctcctgctc gaggagcgtt tcgagatcct gccccagtgc ggccaccgcc
     6781 gccttcacct cgtcccagag tccgaggttg atgaggttgt tacccagatg gggcccgagc
     6841 aggaattcgg ccgagaggta acagacggtg cggctctcct gcttgtaata ggtctccgcc
     6901 gccgatgnnn nnnnnnncgt tccgggacgc catcctggga aatttcgcct gatgacaggc
     6961 gagattgtcg agtatcgcct gcttgagcga cgccacatcg cggcctgtcc ggacaccggt
     7021 cgccgcctca cactgttcgt gatgtttctt ctttgtcatg accttcggac ctcccttccg
     7081 tgacaggtga gggtaacaca tccaagaaca aaaggcaaat cacttgacgg agcagccgtt
     7141 tcggcatacc atcggcgcga acacacgggg gtcattcatg cattcgccgc gcctcgcgac
     7201 catagcgatc gtcgccgtat cggtcctctt cctcgcgacg ctcgcgacgc tctccgtttt
     7261 cgtacacccc tctgccgacg atttctgcgc cgcggtccgg gtcagggatc tcggtttctg
     7321 gccggcacaa tccgagatat accgcaactg gtcgggccgc tattcctcct ccctgctcat
     7381 tttcttcctt cggtggaccg actcgctgcc cctgtaccgt ctcgctcacg cccttcttct
     7441 ccttttttcg gtcttttcct gttttttcct cttcgagaga ctcctgcccg tcggcacgcc
     7501 acggcgcgta cctctcaccg gggccggcat cttctccttt cttttcttcg ccgggacgcc
     7561 gagtcttgtc gaggcggcct attggctggg cggcgccgaa atgtaccatt tctcgacctc
     7621 gctcacgctc ctcctcgtcg gggcatcgct ttccccccgc aggatactgc atgaacgggt
     7681 cgatcacttc ctgctcgcgc tgtgcatcct tatcgtcgcg ggatcgaacg agatatcgat
     7741 gctcctgctc atcatcatcc tcgcggcggc gacgctcttc cgttccctgc gggcgggacg
     7801 gatcgaccgg cgaagcgcct tcctgctcgg cctcgcggcg ctgttctcgc tcggcgtcat
     7861 cctcgctccc ggcaatgcgg cgcggtcgct ttcctacacc caccaccacg acatcctgtt
     7921 ctcaacgggc gccacggtcg ccctgctctc ggccctcgcg ccgatgtggc tcatgagtcc
     7981 gctcctgtgg atctgttccc tttgctatct ttggtggcgt cgtaccgggg cactgtcccc
     8041 ctcgcttccg gcacaccgga ggatgccgac gatcatcctg ttcctcctgc tcctggcggg
     8101 taccgtgttc ccatcgnnnn nnnnnntcaa catcacctat gcggtcttcc tgctttttct
     8161 gctctacctg cttgaaacgg tcatgcttcc gtggatcacg accaaattcc cgacgcatac
     8221 acgcttttac ggtcgggcca tcttcgtcgc actggccttt ttcgtcctgt actcggtcgt
     8281 gatagcatcc ataccccggc agaaggaatg gagccgcgat atcgaacaat acctcgctga
     8341 gccctcccgt gccgttgcc
//
LOCUS       DAOQUE010000063         8321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_9513_length_8321_cov_4.103194, whole genome shotgun sequence.
ACCESSION   DAOQUE010000063 DAOQUE010000000
VERSION     DAOQUE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9513_length_8321_cov_4.103194"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..>8321
                     /locus_tag="PKH10_03115"
     CDS             89..>8321
                     /locus_tag="PKH10_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT27147.1"
                     /translation="MDVRGGVEPFLYHVVRGTQYEWRTNTNSPWVSFNTEITVIKKNS
                     PPYTCNSGTVDGTIVAYNDDDSTATDGRSIARWTADFTGIVAVLVTERYCAEHDVADT
                     GVFLEWRTLPIVATPCALCTDPDPAVEVGSVTTSDVFQSVATNLKAGDFVTFPVTPTQ
                     YYQWTTCGSPDPTYGTTLTLFFKNDAPIVDDTYTCTITAPATIGALGGTPVAFNNDGA
                     YEPPDLSCANGLSTMGWWVNPALTDKNPHIVAVLVNESVCRVHDDTYPPSGVTLSWRT
                     IDPPEQCDNCSRQYPVSIQKNPATKPDWSPVTNLMRGGDYAFFEVEESYVYEWSTCET
                     NLSGEYFDTQLTLRRDTCNGALLSYNNDNAIEGTYCDGNPQRSKFSTVQWYAPFTGTV
                     AILLSEKYCQNNYAPATTLKWRRYSASADCVTCIEDDPATTLVVEGYAGSYTAPVNIG
                     DVNLISNISDGYFARITNIVANATYEFTTCGTGGAATIDTQLTLRGAAALTDPNCSSP
                     LLAYNDNGDAEVLPVVADKDAFTFTSSVPHWLQNGTKVRFSASALPVGIAKDTDYYII
                     ERSNTTFKVAATSTSSTPVAFSTAGTDVLAHVICGQRSRLIWTAPADLNGKTVSISMH
                     DFPCVQSGLKNAPVRVKKLKGLRFTDNLDPAGTGDTVINDNTTGLFWWVPPAPLVLDW
                     QGALDHCATTLNSGSGYAGYKTWRLPTINDLVSIIDFNLQDPATSMPRITSLAEEKQH
                     WAWSSTTYIFGDPGDSSGASYHFAWTANLQDGRTYAEVKEPYAYEETTDDALVPDDDQ
                     IVAPAKTPVTLCVRDTSVIGVHRNFVAGGTSADPYVVGEQAPVTGWACDKSYYSDRAN
                     LGTPLPHLYGYVEVWTSPYTDPLPPFKHYPSQKIWTSPDDFLINRTSTNAEAANNCGL
                     APTGTGYNFSYDLTAHVEGPTGIYRRVQDELVLNGRRPVWPLLVYAYAVPLDGNGVPQ
                     RMGEAPGVKLWLSPQGVYFFGDKLLCGDGYKQIGEDCDDANSSNEDGCVTLPNDPYTG
                     VCTNASCGDGYIGTYGPVVEVCDKPTCAYPSSSYQAQCPGASDVECAADCQSLSDCDW
                     HCCNDQEKNGCQTSCTIYPSCSKDCTVNFSKVSQTKSCTPTCGVQTCEILYGLSGCTA
                     SCPGLSKQAECTITYDSESKTNSCSTSCSTSTQSCTPATQAGCPYGVGLTAPVESGNC
                     DHPYSGTISASKTNSCTPSSCTVDCMATCWATNCTPPGVGKTCTASCTPTCAVGGPTR
                     SCTRECTPGSLTAKPSYTCSKTLYGDDRPEECDGGDGGSGDLQECHELNAKYYDREVT
                     ADCNSDCRIATTVDLDDICPWCGNNIVEENYTIDTVPGGEVCDPTVPDSMYCINLNHP
                     TIPSFLDNGGVVANCATCAPAEDDIRDNCRWCGDNRFTDISPEMCDPTTTEDQNVYGG
                     PKIKCSVWGAKSGFVYYDDLLLTCPDNCTQYTADGNPSSGAGDPDNPQAYCHYCGDGV
                     LTPSKETCETQPLAVGDPYNIPPYIGKDCGDYGANNLTDPAAFPYWPSYTPPGYLNLA
                     ACVACGPSYDYTTCDYCGDGDINGPETCELGDWVRCVDITPNPGYYATNDGVTGDGSD
                     TVTYCNNSSNADRHCKEWCVGDPAVTGCPAEWICPYCGDGLYTSRAETCDPSSPIAGI
                     GGGVITCAVYWNNRADVPLDGIAMTSGELPKFHPYGDTLVECRPGCMNHYTQRTLCRY
                     CGDGLTQDDAIGKEECDPTDTSNTHYCKELDDTTVNPPKIFYPLFTVGCAPDCTLQDP
                     DDPSFTGCPRCGDGKYTADYTAAGHPELPLEKCDHNATDGFDSFGSREIYCTLWCSQD
                     EDATLRNCYDYPGATRRTVTCSETPKDCSLYEQSSEAAKKCRGCGDGENLLYNSDLVM
                     ALHFEEPSGTTLYDAAPGTAANAVPAAGAFSGVTLNQSGRRAQGKAISFTHSNNLRIN
                     DAAKLDLSGDMTFAFWFKQSAFYTPPIAGAPYPILVKGHGWESNYGFWMHNDGVNNII
                     KFSFHSAEVTGCGITTTAQDWTQWTHVAAVRSGQQLTMYVNGAQVNTISCATINPPVN
                     SHPITIGEDTPHPMHNNVNYGVTGYMDELFLYKRALSQPEVTFLVNRGASVAAEYEYC
                     DDGNVSNGDGCNSNCGIETDYVCTGSPSVCTVPSGLTCEAGWTYNPTTQKCYKYFSTA
                     RIFTAAQADCGTNSGNLVSMADATENSFVRTSVVDPASSGAQYWIGLRQPETAVGTVE
                     TYNAGSTYNKIYCGTTTTSLGETYSYNDTAFTWVAPVTGYYTFRVGTTTSSFDIYFAY
                     YDGYMGSYYTADAYGEGESETLSYQYMYAGYTNTIYVGGYNSADAGQFCLDIIYPYTS
                     EVPWEWTDGAAYVYGGWAGGQPDNAAAVQNCAYVPSGAGVTTWDDLACNSSIPYVCKK
                     GSVPSICGDGTKTPDEECDNGASNGPCGSCTSACKNQAFPPCGDGTLCAGETCEDNLP
                     GQPADWATGADAYDGCFEGCVAGYHPIGIHRAASTALNGISGWACDPDEWSQPVTVYV
                     ELRYTNQNASETCANDNPSSPTPPCTVAVQSYLDNFPVNKVSSAEVVAACGGSTDGGS
                     IHDFALSVSDFSNPALTVARSPYWAKVYAIDPAAPTDSRLKPLNDTPLHINICGDGIV
                     AEGEECDEGAAGFSDECDGCTSDCKVIPAYSCGDGVVCTNHGIIEECDDGVTNNSYDK
                     PCTEDCRINPCAYGSGWYFNVANDRCYAMTPG"
BASE COUNT         1758 a         2677 c         2306 g         1580 t
ORIGIN      
        1 tgccggtttg gcgtcctcgg agaacacgcc cgatccggcc gtgttctccg aggacgccat
       61 accggcatgg acctcttcgg atgcgaccat ggatgtcagg ggcggggttg agccgttcct
      121 ctaccatgtg gtcagaggca cccagtacga atggcgcacc aacaccaaca gcccgtgggt
      181 ctcgttcaac accgagatca ccgtgataaa gaaaaatagc ccgccctata cctgcaacag
      241 cgggaccgtc gacggcacga tagtcgccta taacgacgat gacagtaccg cgaccgacgg
      301 tcggtcgata gcccgttgga ccgccgactt caccggtatc gtcgcggtct tggtgacgga
      361 gcgatactgc gccgaacatg atgtggccga cacgggagtg ttccttgagt ggagaacgct
      421 gccgatcgtt gcgaccccct gcgccttgtg taccgacccc gatccggcgg tagaggtcgg
      481 ctcggtcacc acgagcgatg tgttccaatc ggtcgcgacc aacctcaaag ccggcgattt
      541 cgtcaccttt cccgttacgc cgacccagta ctatcaatgg accacctgcg gcagtcccga
      601 cccgacctac ggcacgaccc tgacgctgtt cttcaaaaac gacgcgccga tcgtcgacga
      661 cacctatacc tgcaccatta ccgccccggc gaccatcggc gcgctggggg gcacgccggt
      721 cgctttcaat aacgacggcg cctacgagcc gcccgatctc tcgtgcgcca acggtctgtc
      781 gaccatgggg tggtgggtga atcccgccct gaccgataaa aacccccata tcgtggcggt
      841 gttggtcaac gaatcggtct gccgcgtgca cgacgacacc tatccgcctt cgggcgtgac
      901 gctcagttgg cgcaccatag acccgcccga acagtgcgac aactgttccc ggcaataccc
      961 ggtgtccata cagaaaaacc cggcgaccaa acccgattgg agtccggtga ccaaccttat
     1021 gagaggcggc gactacgcct tcttcgaggt cgaagagagt tatgtgtacg aatggagcac
     1081 ctgcgaaacg aacctgtcgg gagaatattt tgatactcag ttgaccctgc ggcgcgatac
     1141 ctgcaatgga gcactactgt cctacaacaa cgataacgcc atcgaaggca cctactgcga
     1201 cggcaatccc caacgatcga aattctcgac cgtccaatgg tatgccccct ttaccggcac
     1261 cgtcgccata cttctcagcg agaaatactg ccagaacaac tatgcccccg cgaccacgct
     1321 caaatggcgc cgttacagcg cctccgccga ctgcgtcacc tgcatagagg acgatcccgc
     1381 cacgacgctg gtcgtggaag gatacgccgg ctcgtatacg gcgcccgtga atatcggcga
     1441 tgtcaacctg atcagcaata tctccgacgg ctacttcgcg cgcatcacca acatcgtggc
     1501 gaacgccacc tatgaattca ccacctgcgg caccggcggc gccgccacca tcgatacgca
     1561 actcaccctg cgtggcgcgg cggcattgac cgatccgaac tgctcttcgc cgcttcttgc
     1621 ctacaacgac aacggggacg cggaggtcct gccggtcgtc gccgacaagg acgctttcac
     1681 ctttacctcc tcggtccccc actggcttca gaacggtacg aaggtccgtt tcagcgcgtc
     1741 cgcgctcccc gtgggcatcg cgaaggatac cgattactac atcatcgagc ggagcaatac
     1801 gaccttcaaa gtggccgcca catcgacaag cagtaccccg gtcgccttct cgacggcggg
     1861 gaccgatgtt ctggcgcatg tcatctgcgg ccagagatcg cgccttatct ggaccgcccc
     1921 cgccgatctc aacggcaaaa cggtcagcat atccatgcat gacttcccct gtgtccagtc
     1981 gggcttgaag aacgcgccgg tccgcgttaa aaaactgaaa ggtctccgct tcaccgataa
     2041 tctcgacccc gcgggcaccg gcgacacggt catcaacgac aataccaccg ggttgttctg
     2101 gtgggtgccc cccgcgccgt tggtgttgga ttggcaggga gcgctcgacc actgcgccac
     2161 taccctcaac agcggaagcg gctatgccgg ctataagacc tggcggcttc ccaccatcaa
     2221 cgatctggta tccatcatcg atttcaatct tcaggatccc gccacgagca tgccgcggat
     2281 cacctccctt gccgaggaga aacagcactg ggcgtggtct tccaccacct acatcttcgg
     2341 cgacccgggc gatagttccg gcgccagtta ccatttcgcg tggaccgcca atctgcagga
     2401 cggccgcacc tatgccgaag tgaaagagcc ctacgcgtat gaagagacca cggacgatgc
     2461 cttggtcccc gacgacgatc agatcgtcgc ccccgccaag accccggtga ccctctgtgt
     2521 gcgtgatacc tcggtcatcg gcgtccacag gaatttcgtc gccggcggca cctctgccga
     2581 cccctatgtg gtcggagaac aggcgccggt caccggctgg gcctgcgata aatcctacta
     2641 cagcgaccgc gccaatctgg gcaccccgct tccccacctg tacggctatg tcgaggtgtg
     2701 gacctctccg tataccgatc ccctgcctcc cttcaaacac tacccttccc agaagatatg
     2761 gacctcgccg gacgacttcc tcataaaccg cacctcgacc aacgcggagg ccgccaataa
     2821 ctgcggtctg gcgcctaccg gtaccgggta caatttctcg tatgacctga ccgcccatgt
     2881 ggaggggccg accggcatct atcgccgggt gcaggacgaa ctggtcctca acggtcgccg
     2941 cccggtctgg cccctgctcg tctacgccta cgccgtgccg ctcgacggga acggcgttcc
     3001 tcagcgtatg ggagaagccc cgggcgtcaa actctggcta tcgccgcaag gcgtctattt
     3061 cttcggtgac aaactgctct gcggtgacgg atacaaacag atcggcgagg attgcgacga
     3121 cgcgaattcg agtaacgagg atggatgcgt gacattgccg aacgatcctt acaccggcgt
     3181 ctgcacaaac gcatcctgcg gcgacggcta tatcggaact tacgggccgg tggtcgaggt
     3241 gtgcgacaag ccgacctgcg cgtacccgtc gagttcgtac caagcacagt gccccggggc
     3301 ttccgatgtg gaatgcgccg cggattgcca gtccttgagc gattgcgatt ggcactgttg
     3361 caacgatcag gaaaagaacg ggtgccagac ctcttgtacg atatacccct cctgcagcaa
     3421 ggactgcacc gttaatttct ccaaagtatc gcagacaaag tcatgcacgc ccacctgcgg
     3481 cgttcaaaca tgcgaaatac tttacggact ttccggttgc accgcctcct gtcccggtct
     3541 gtcgaagcaa gcggaatgca ccatcaccta cgacagtgag tccaaaacta acagttgttc
     3601 gacctcctgt tctaccagca cgcaatcttg tacccccgcg acacaggccg gttgtccgta
     3661 cggggtcggt ttgaccgctc cggtggaatc cggaaattgc gatcatccgt attcggggac
     3721 aatttccgca agtaaaacga acagttgcac tccttcctcc tgcaccgttg attgcatggc
     3781 tacctgttgg gccaccaact gtacccctcc gggcgtaggc aagacctgca ccgcgagttg
     3841 taccccgacc tgcgcggtgg gaggtcctac ccgtagttgc acgagagagt gtactcccgg
     3901 ctcgctcacg gcgaaaccct cctacacctg ttcaaaaacc ttgtacggag acgatcgacc
     3961 tgaggaatgt gacggcggcg acggagggag cggcgatctt caagagtgcc acgaactcaa
     4021 tgcgaaatac tatgaccgcg aagtgaccgc cgactgcaat tccgattgcc gcatcgccac
     4081 cacggtcgat ctggacgata tctgtccgtg gtgcggcaac aatatcgtcg aggaaaatta
     4141 cacgatagat acggtcccgg gaggcgaggt atgcgacccg accgttcccg acagcatgta
     4201 ctgcatcaat ctgaaccacc ccacgatccc ttcgttcctc gacaatggcg gtgtggtggc
     4261 gaactgcgcg acctgcgccc cggccgagga tgatatccgg gataattgcc ggtggtgcgg
     4321 cgacaacagg ttcaccgaca tctccccgga gatgtgcgac ccgaccacga cggaagatca
     4381 gaatgtgtac ggcggcccga aaataaaatg cagtgtctgg ggcgccaaga gcggctttgt
     4441 ctactatgac gatctcctgc ttacctgtcc cgataactgc acccagtaca ccgctgacgg
     4501 taacccctcc tcgggagcgg gcgacccgga caatccgcag gcatactgcc attattgcgg
     4561 tgacggggta ttaactccga gcaaagagac ctgcgaaacg cagccgcttg ccgtgggtga
     4621 cccttacaat ataccgccct acatcggtaa agactgcggc gactatggcg ccaacaacct
     4681 gaccgatccc gccgccttcc cgtactggcc ttcctatacc cctcccggtt atctgaatct
     4741 cgccgcctgt gtcgcgtgcg gcccttcata cgattacacc acctgcgact actgcggcga
     4801 cggcgatatc aatggccccg agacctgcga gttgggcgat tgggtgcggt gcgtcgatat
     4861 cacgcccaac cccgggtact atgcgacgaa cgacggtgtt accggcgacg ggagcgacac
     4921 cgtcacctac tgcaacaaca gttccaatgc tgaccggcac tgtaaagaat ggtgcgtcgg
     4981 cgatccggcc gtgaccggtt gtccggcgga atggatctgc ccgtattgcg gggatggcct
     5041 gtacacctcg cgggccgaga cctgcgatcc ttcgtctccc atcgccggca tcggcggcgg
     5101 ggtgataacc tgcgccgttt attggaacaa ccgtgccgat gtgccgctgg acggcattgc
     5161 gatgacgagt ggagagttgc ccaaattcca cccgtacggc gatacgctgg tggaatgccg
     5221 cccgggctgt atgaaccact acacccaacg caccctgtgt cgctattgcg gcgatggtct
     5281 tacccaggac gatgcgatcg ggaaggaaga atgcgatccg acggacacca gtaataccca
     5341 ttactgcaag gaacttgacg acacaacggt gaatccgccg aagatattct atccgctctt
     5401 cacggtcggt tgcgcccctg actgtacgct tcaggatccc gacgatccct cctttaccgg
     5461 ctgtccgcgc tgtggcgacg gcaaatatac ggcggattac accgccgccg gacatcccga
     5521 actgcctctc gaaaaatgcg atcacaacgc caccgacggc tttgattcgt tcgggtcccg
     5581 cgagatatac tgtacgcttt ggtgctcgca ggacgaagac gcgacattga ggaattgcta
     5641 tgactatccg ggcgcgacgc gacggacggt gacctgcagt gaaacgccca aagactgctc
     5701 cctctacgaa cagtcgtccg aggccgccaa gaaatgccgt ggatgcggcg atggggaaaa
     5761 cctcctgtat aacagcgatc tggtcatggc tctccatttc gaggagccga gcggcaccac
     5821 gctgtatgac gccgcgcccg gtaccgccgc caacgcggtg cccgccgccg gcgctttttc
     5881 cggcgtcacg ctcaatcaga gcggtcgccg cgctcagggc aaggcgatat cctttacgca
     5941 cagcaacaat ctccggataa acgatgccgc caaactcgac ctttccggcg acatgacctt
     6001 tgccttctgg ttcaaacaat ccgccttcta tacgccgccc atcgcggggg cgccgtatcc
     6061 catactcgtc aagggacatg gctgggagtc gaactacggg ttctggatgc ataatgatgg
     6121 ggtgaacaat atcatcaagt tctccttcca ttcggctgag gtcaccggtt gcggcatcac
     6181 caccaccgca caggattgga cccaatggac ccatgtcgcg gcggtacgca gcggtcagca
     6241 actgaccatg tatgtgaacg gtgcgcaggt gaacacgata tcgtgcgcga ccattaatcc
     6301 gccggtgaac agccacccca tcacgatagg ggaggacact ccacatccga tgcataacaa
     6361 tgtgaattac ggcgtcaccg gttacatgga tgaactgttc ctctacaaac gggccctttc
     6421 tcagccggag gtcaccttcc tcgtcaaccg cggggcgtct gtcgccgcgg agtatgaata
     6481 ctgcgacgat ggcaacgtct ctaacggcga cgggtgcaat agcaactgcg ggatagagac
     6541 cgactatgtc tgtaccggtt ctccgtcggt ctgtaccgtg ccgagcggcc ttacctgcga
     6601 ggccggttgg acctacaacc ccacaacgca gaagtgttat aaatacttct cgacagcaag
     6661 aatcttcacg gccgcgcagg cggactgcgg caccaattca ggcaatctcg tttcgatggc
     6721 cgatgcgacc gagaattcct ttgttcgcac cagcgtcgtc gatccggcct cgtcgggcgc
     6781 tcaatactgg atcggcctcc ggcaacctga gacggcggtc ggcacggtcg agacctacaa
     6841 tgccgggagt acatataaca agatctactg cggcaccacc accaccagcc tcggcgaaac
     6901 ttattcgtac aacgacacgg ccttcacatg ggttgcgccg gtgaccggct actatacctt
     6961 cagggtcggg accaccacct cctccttcga catctacttt gcctattatg acggttacat
     7021 ggggtcttat tacaccgctg atgcttacgg cgagggtgag agcgaaacgc tctcctacca
     7081 gtatatgtac gcgggataca ccaacacgat ctatgtcggc ggatacaaca gcgccgatgc
     7141 cggtcagttc tgtcttgaca tcatctatcc ctacaccagc gaggttccgt gggaatggac
     7201 cgatggcgcc gcttatgtct atggcggctg ggccggcgga caacccgata atgccgccgc
     7261 cgtccagaat tgtgcttatg tccccagcgg tgccggtgtg accacatggg acgatctggc
     7321 ttgcaattcc tcgataccct atgtgtgcaa gaaagggagc gtcccgtcta tctgtggtga
     7381 cggcaccaag accccggacg aagagtgcga taacggtgcc tcgaatggtc cctgtggctc
     7441 ttgtacctcg gcctgtaaaa atcaggcgtt ccccccctgt ggcgacggta ccctctgcgc
     7501 cggagagacc tgcgaggaca atctgccggg acaaccggcc gattgggcga ccggcgccga
     7561 tgcctacgac ggctgtttcg aaggctgtgt ggcgggctat catccgatcg gtatccaccg
     7621 ggccgcctcg accgcgctta acgggatctc cggctgggcc tgcgatccgg acgaatggag
     7681 tcagccggta acggtctatg tggaattgcg ctacaccaac cagaacgcga gtgaaacctg
     7741 cgcgaacgac aatccgtcga gtccgacacc gccctgcacg gtggcggtcc agtcatatct
     7801 cgacaatttc cccgtgaata aagtatcctc cgcggaggtc gtcgccgcct gtggcggttc
     7861 gacggacggc gggagtatcc acgacttcgc cctttcggtc agcgacttct ccaacccggc
     7921 cttgaccgtg gccagatcgc cgtactgggc gaaggtgtac gccatcgatc cggccgcgcc
     7981 taccgatagt agacttaagc cgctcaacga tacgccgctg cacatcaaca tctgcggcga
     8041 cggtatcgtg gccgagggcg aggagtgcga cgagggcgcc gccggcttca gcgatgaatg
     8101 cgacggctgc accagcgact gcaaggtgat ccccgcctac tcctgcggcg acggcgtggt
     8161 ctgcaccaac cacggcatca tcgaggaatg cgacgacggc gtaacgaaca acagttacga
     8221 caaaccctgc accgaagact gccgcatcaa tccgtgcgcg tacggctccg gctggtactt
     8281 caatgtcgcc aacgaccgct gttacgcgat gacccccggc g
//
LOCUS       DAOQUE010000064         8297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_9547_length_8297_cov_4.607377, whole genome shotgun sequence.
ACCESSION   DAOQUE010000064 DAOQUE010000000
VERSION     DAOQUE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9547_length_8297_cov_4.607377"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKH10_03120"
     CDS             complement(<1..196)
                     /locus_tag="PKH10_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27148.1"
                     /translation="MLSLQNIRSAEAARSYFSKDNYYSKDTFRAEYFGEGARILSLLD
                     KEVTQERFVERPVDHDVRKRL"
     gene            complement(196..1881)
                     /locus_tag="PKH10_03125"
     CDS             complement(196..1881)
                     /locus_tag="PKH10_03125"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HNT27149.1"
                     /translation="MPAKTRTASDALQSRLSTAKTLVLALAFALMASLLFGMQAYKKF
                     TIEPERAIAKCYFQMWVLDRTEKECRVMEETFILTPDLIREKFGDRLQKVKTVLFIIL
                     GVGVVAFILFTFLFYKVFLLMDRGKTADVYLRGARKATSDELRGFLEKEGTASPITIG
                     GVPIVAGSETTHFLIAGATGVGKSVAIKELLDHARAARQRAIVYDPSGEFVEAYYREG
                     TDLILNPFDKRAPRWNIFEDIGSEFDYADVVQSLIPMPANTNDPFWVNAPRGVLESML
                     RNMKLRNNYSNRYLYETLTTRSISELHRELKGWEGAAYLDPSNDKTANSVRSVLSPYL
                     LPFKYLSDTGGDPFSLKKFIADDSIDRWVFIRVDMDQLNALRPLISLWLDLAITATLS
                     LEVKPSRRLWFVMDEFATLQKVSKVKDLLDKGRKYGACAILGMQNISQIKESYGKEGA
                     TSMVTNCKTWVIYNTPDSETAKYLAENIGNEEVLATKASQSESSDAKGGGSQSTTTNY
                     QTEKKLVVMPEEIMYLPNLAAFLKVSGDHPVALISLEWKARPTRAPKFIPRET"
     gene            complement(1905..2309)
                     /locus_tag="PKH10_03130"
     CDS             complement(1905..2309)
                     /locus_tag="PKH10_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27150.1"
                     /translation="MVRETLVSARIPEQIRERLKKESDLHGMSESAYLKTLLELHFGD
                     EDNRFITYEGRYLKALLERNTYNIYAIMEHLKRTKDPAWDGKEYHQAAKTKALKSEKD
                     CHAIALPDTPEKGEKDPTGPEASGKGNDNPDL"
     gene            complement(2835..3044)
                     /locus_tag="PKH10_03135"
     CDS             complement(2835..3044)
                     /locus_tag="PKH10_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27151.1"
                     /translation="MREKGTEAAVRTLEVGDYRAAKIHLDITFDRVKKEVEEMKKEKG
                     LSVQQMTHKPAHDSSVKKTQTGPEL"
     gene            complement(3288..4343)
                     /locus_tag="PKH10_03140"
     CDS             complement(3288..4343)
                     /locus_tag="PKH10_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNT27152.1"
                     /translation="MKPLLKDIYSQLDRAKLLAELRPELSGDHYILTCPSCGKREAYL
                     YLNSHLIICNRQNNCGYKKSLWDWTAARYGYGQKETFAHLARLAGVTLDDDEGGAHRD
                     PTTLLRNTQKACAKALFSIPDNPALRYLRDRRRWSEEEIRASGIGYHPGSEALRIALN
                     ADHTTEEIDALPLLATKLFSTKYRLIIPYHRDGVIAGFIGRLLEEDLDQSKYRFPKNT
                     KVPLVDFEPATEVTIVEGILDARLARVRGIRSVFASCTNQLSGAQLDTLTANRNVIKR
                     IRLLFDNDAAGRKGTFTSIVKLLGDGFTDIRVGELPEGSKDLEEYLTGTKKTTIEEIL
                     FLPYYEWLSLHYPVTVS"
     gene            5786..7369
                     /locus_tag="PKH10_03145"
     CDS             5786..7369
                     /locus_tag="PKH10_03145"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HNT27153.1"
                     /translation="MTKKKDPESKRLVSKTNEGYKAESIKIPKIVVEEERYNLEKDDS
                     LIKYEFNFIELPFFTKDDRIGKNVSKKYSFSGTSYMQVTPSQEEESGHKIPQDFDEKI
                     FYAILRLYRRYNRKRIVTTIYELLELAGAGVSTKDYDRVKESIFRLQGTSIKCSNILY
                     NAEDRKRMSDTQLLKILQYARIVKVEDISAKDRADYKERIKANMKEVVVVELSDFIER
                     NILAKGYLNYDAEKLIDIANATARKMYLLIEKWRGWEKSDIIRRSCRFLASRIPLSWE
                     DKNVSGSIKSIEKAAQELKIKGLIGDYQLSRAGGVKDTEIIFFFEGATASARAQAIRK
                     RNEEQIQETGHEGIQILDVTYDERQSTIFDSNGTAKDPDLLNEPSELAALIELVPAVS
                     RTKSLQALIARYLKEKGGEYVSSNIEYCRKNARDFGGMLPGALQKDFGEGIRLARAAE
                     EQERSVKKRAREQASNTEQERLAFEEWWRGLTDKEKAEVDIRAAEMAEAAGLSKTVMG
                     LATVANRVKVYKERKKKAL"
     gene            complement(7426..7938)
                     /locus_tag="PKH10_03150"
     CDS             complement(7426..7938)
                     /locus_tag="PKH10_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27154.1"
                     /translation="MPFDKNRLKRPNADKVLEKFISGATADVALKPKKGKEEQPQGKA
                     VKKSAVPRSSEKVKAKAGTKKKEKPTPQPLAPAPQEKATRKTPEPKIPAEPIPEKPQT
                     PSAADKTPVVSVVPSLQETPLVLPNDIKSGITLNLYHNLFKTFKALCHRDGKTPSDLF
                     NKFMNEYLGG"
     gene            complement(7925..>8297)
                     /locus_tag="PKH10_03155"
     CDS             complement(7925..>8297)
                     /locus_tag="PKH10_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNT27155.1"
                     /translation="FRNALIASDLLIIPVTPSPVDLWSTEDVFKMLEELRTAKDIKAG
                     ILLNMVQPGVSITTDVLEILGEYADKYSLFLFMSMIYFRIGFKEAFAEGCSVTEMKGE
                     KFAKASAEIQELFREVNHAVR"
BASE COUNT         1704 a         2094 c         2467 g         2032 t
ORIGIN      
        1 gaagccgttt cctcacgtcg tgatcgacgg ggcgttcgac gaaccgttcc tgcgtcacct
       61 ccttgtcgag cagcgacagg atgcgggccc cttccccgaa gtattcggcg cggaaggtgt
      121 ccttcgagta gtagttgtcc ttggagaagt aggaccgcgc cgcttccgcc gagcggatgt
      181 tctgaaggga aagcatcagg tctcgcgcgg tatgaatttc ggggcgcggg tcgggcgcgc
      241 tttccactcg agggagataa gggcgaccgg atggtcgccg gataccttga ggaaggccgc
      301 gaggttcggg agatacatga tctcctccgg catgaccacc agtttctttt cggtctggta
      361 gttggttgtg gtcgactgcg agccgccccc tttcgcgtcg gacgattccg actgcgatgc
      421 cttggtggcg agtacctcct cgttaccgat gttctcggcg agatatttgg ccgtctcgct
      481 gtcgggggta ttgtagatga cccaggtctt gcagttggtc accatcgagg tggccccctc
      541 tttcccgtag gattccttga tctgcgagat gttctgcatc ccgaggatgg cgcaggcgcc
      601 gtatttgcgg cccttgtcga ggaggtcctt caccttcgag accttctgga gcgtcgcgaa
      661 ttcgtccatg acgaaccaga ggcggcggga gggcttcact tcgagggaga gggtcgcggt
      721 gatcgcaagg tcgagccaga gtgagatgag gggacgcaag gcgttcagtt ggtccatgtc
      781 gacccggatg aagacccaac ggtcgatcga gtcgtcggcg atgaacttct tgagactgaa
      841 gggatcccct ccggtgtcgg agaggtactt gaacggcagg aggtagggcg agaggaccga
      901 ccggaccgag ttggcggtct tgtcgttgga gggatcgagg taggcggccc cctcccaccc
      961 cttcagttct ctatggagtt ccgagatcga gcgggtggtg agcgtctcgt agaggtagcg
     1021 gttcgagtag ttattacgga gtttcatgtt ccgcagcatt gattcgagga cgccgcgcgg
     1081 ggcgttcacc cagaaggggt cgttggtatt ggccggcatg gggatgagtg actggaccac
     1141 atcggcgtag tcgaattcgc tcccgatgtc ctcgaagatg ttccaacggg gagcccgttt
     1201 gtcgaacggg ttgaggatga ggtcggtacc ctcccggtag taggcctcca cgaattcacc
     1261 cgacgggtcg tagacgatgg cgcgctggcg ggcggcacgg gcatggtcga ggagttcctt
     1321 gatggcgacc gatttgccga cgccggtagc cccggcgatg agaaagtggg tcgtctcgga
     1381 tccggcgacg atggggacgc caccgatcgt tatcggggag gcggtcccct ccttctcgag
     1441 aaagccccgc agttcgtcgg aggtcgcctt acgggcgccc ctgaggtaga catcggcggt
     1501 tttcccccgg tccatgagga ggaatacctt atagaagagg aaggtgaaga gaatgaaggc
     1561 gaccacccct actccgagga ttatgaaaag gacggtcttt accttctgga gccggtcgcc
     1621 gaatttctcg cggatgaggt cgggggtcag gatgaaggtc tcctccatga cccggcattc
     1681 cttctccgtg cggtcgagga cccacatctg gaagtagcac ttcgcgatcg cccgttccgg
     1741 ttctatggta aacttcttgt aggcctgcat cccgaagagg agggaggcca tgagcgcgaa
     1801 ggcgagcgca aggacaagcg tcttggcggt agagaggcgg gactggagtg cgtcactggc
     1861 ggttctggtc ttggccggca tggggctctc ctttcgttat tgagttagag gtcggggttg
     1921 tcgttccctt taccggacgc ttcggggccc gtagggtctt tctccccttt ctccggtgtg
     1981 tcggggagcg ctatcgcatg gcagtccttt tcggacttga gcgctttcgt ttttgccgcc
     2041 tgatggtact ccttcccgtc ccatgcgggg tccttcgtcc gcttgagatg ttccatgatc
     2101 gcatagatgt tgtaggtgtt ccgttcgagg agcgccttga ggtagcgacc ctcataggtg
     2161 atgaagcggt tatcttcgtc gccgaagtgg agttccagaa gcgtcttgag ataggccgac
     2221 tcggacatgc cgtgcaggtc gctctccttc ttcagccgtt ctcggatctg ctcgggaatc
     2281 cgcgcgctga ccagtgtttc tctcaccatc tgttcctccg gtagacaatt gtagacaacc
     2341 ttgacaaaag cacgctaaca caccaagcta tagatgtcaa gattttagta tgttgacatt
     2401 tgtataccat atcttttctt ttttttcagc acggtagaca tttgttacac tcctgttttc
     2461 tatggtcaac atttgtctac cgctgtatac cgaaaggttg cgtggtgtgt gataattatg
     2521 ggaaagaggg ctagaggagg cgcaaagcga tctcctcgac gaccgaaaga ccggcgtagt
     2581 tccgggcggc gcagggcgac gaacgggacg aagcggcgtg ctggacgtgc gagtgtgggc
     2641 gcagcccacg ggaccggaaa acggacagaa cgatgacgct ccttgtaccg actatcggaa
     2701 cagaatgaag gtgctttttt actgatggtc ggtacatttc ggggccgttt ttgtccggag
     2761 gtatggaacg gaatacgacc ttttttgttt cgaggtaccg gacaaaaaag gtgtgtgttt
     2821 gtgaggagta tcggttagag ttcggggccg gtctgcgtct ttttgactga cgagtcgtgc
     2881 gccggtttgt gtgtcatttg ttgaacggaa agccccttct ctttcttcat ctcctcgacc
     2941 tctttcttta cacggtcgaa ggtgatatca agatgaattt tggcggcccg gtagtcccct
     3001 acttcgaggg tcctgacggc ggcttcggtc cctttctcgc gcaaaaaagt cttttatacc
     3061 gttacacgct tttcttcatt ttatagatct tcctgttccg cctttcttcc aaaaacatat
     3121 tttctcttgc tcgcctcttg ggaccatttc ttttcagaaa gaaggccggg ggagtccccg
     3181 ttttggcctg tttttgtttc tctcttttac gatctttcgt gttgttcgtc gattaaaatc
     3241 ggataacttc ttgtgacgag cttatgcttc tttttcgact aagaatgtta cgacaccgta
     3301 accggatagt gaagagaaag ccattcataa tatggtagga aaagaatctc ttcgatggtc
     3361 gtttttttgg ttccggtgag gtattcttca aggtccttgc tgccttcggg cagttcccct
     3421 acccggatgt cggtgaagcc gtcgccgaga agtttcacta tcgaggtgaa ggtccctttg
     3481 cggcccgcgg catcgttgtc gaagaggaga cggatccgct ttatcacatt ccggttcgcg
     3541 gtcagggtgt cgagctgcgc gccgctcaac tggttggtgc aggaggcgaa gaccgatcgg
     3601 atgccgcgta cgcgggcgag ccgcgcgtcg aggatcccct cgactatcgt cacttcggtc
     3661 gccggttcga aatcgacgag cggcactttc gtgttcttgg ggaaccgata cttcgactgg
     3721 tcgaggtcct cctcgaggag acggcctatg aaaccggcga tgacgccgtc ccggtgatac
     3781 gggatgatga gccggtattt cgtcgagaag agtttggtcg ccagaagcgg cagcgcgtcg
     3841 atctcttcgg tggtatggtc ggcgttgagc gcgatgcgca gcgcctcgga gccggggtgg
     3901 tatccgatgc cggaggcgcg gatctcctcc tcgctccagc ggcgccgatc acggagatac
     3961 cgcagggccg gattgtcggg aatactgaag agcgccttcg cgcaggcctt ctgcgtgttg
     4021 cgcagaagcg tcgtcggatc gcgatgcgcg ccgccctcgt cgtcgtcgag cgtgacgccg
     4081 gcgaggcggg cgaggtgcgc gaaggtctcc ttctggccgt agccgtaccg cgccgcggtc
     4141 cagtcccaga gggatttctt gtagccgcag ttgttctggc ggttgcagat gatgaggtgg
     4201 gagttgagat agaggtaggc ttcccgcttc ccgcaggagg gacaggtgag gatgtagtgg
     4261 tcaccggaga gttcgggccg cagttcggcc agaagtttcg cgcggtcgag ttgggagtag
     4321 atgtctttga ggagcggctt catgttccct ccggccgggc ctagtataca agaccacccg
     4381 gaaaaggcaa atcggttctc ccgttttcat agaagagaaa aatgaggcat tatcgcggca
     4441 gataatccgc cgttttctga tatgaaatca atatgttgta gactgtttct caataccgta
     4501 aaaagcgggg gcgccctctc gcgaaacagt cccgccgtag tgagtctcgg gtcgaaccac
     4561 gataccttcg cggaccgggc tcgtcggatg gatgtccggg tttcgaccgt tgccggactg
     4621 gtcgcgtggc ttcgtttcgg gtggagacac gataccgatc acaccctccc gaacagtttt
     4681 ccccgccggc cgcgggttgg agcggacggc gcgccgcgcg gtgccgaggt gtcatcgggt
     4741 ggaaacacgg tatcccgcag aagtcgcggc tttcttgggc cggttgattt ttttcctttt
     4801 tgttggaatt atttttgtgg ggttaagagg aaaaatgtag ttttgtgttt gtgccgaacg
     4861 gagtgaggct tttgtgtttt gggtatgggt gcgacgggtc gctatcccat cctttgttta
     4921 gagatggtgg tttgggtatg gttcgtggct ttgctgttta ggtgcctgct gtagctttag
     4981 cgtaagcagg cgggttgggt atgggagcga cgataggagc gatcccatcc gaattggggg
     5041 ggttctgttt tcgcgcgaaa acgcgacttc gcttcgctcc gtcgcatggt gtattctgcc
     5101 ttttgcattt cttcgctgtg cgacggaacg acgccaacct tggatcggag ccgtctccct
     5161 tcccctatcg cggcgccttg cgcctacgct acgctacggc ggcacaagac ggcgcctcgc
     5221 gcgctccgcg cgacaacggt cgccggctcc gccggcattg aattaaattt cgcgcgggat
     5281 cggaagttgg ttgtagttgt ttttttcttt tgattgttaa tatagttact atatagtata
     5341 ggatagcatc gggtggatac ctgatatttt atcgggtcga accacgatat cttatctgct
     5401 ggatacccga tacaagttct gatggaaccc cgagggcttt cgtgtggaaa cccgatagtg
     5461 gccggatgga accacgatat ttatcgggtg gaaactcgat agatgctcct tcctgtcttt
     5521 ttcttctttc ctgatcggct ggaatcctga tattttcggc tggaaacacg gtatccccta
     5581 tcctctttct gatggaaccc cgatgttttc tgccggaaac ccgataaaaa acttgcattt
     5641 ctgctggaga cacgatattg ttccttcaag gatggaggag gcagatcgtg tctttcaaaa
     5701 ctctcgctcg ctttaactac ttgttcattc atgtgtttta tgttgcttct gatggaacca
     5761 cgacactctg cgcgttgaaa aatcaatgac aaagaaaaaa gaccccgagt cgaagaggct
     5821 cgtctccaag acgaatgaag ggtacaaagc cgaatcgata aagatcccca agatcgtcgt
     5881 cgaagaggaa cgctacaatc tcgagaagga tgattctctt atcaagtatg agttcaattt
     5941 catcgagctc cccttcttta ccaaggatga ccgcatcggg aagaatgtct ccaaaaagta
     6001 ttccttctcg gggaccagtt acatgcaggt gaccccctct caagaagagg aatcggggca
     6061 caagatacct caggattttg acgaaaagat cttttatgcc atcctgcgac tttatcgccg
     6121 gtataatcgc aaacgcattg tgaccaccat ctacgaattg ctcgagttgg caggcgccgg
     6181 cgtctccacc aaggattacg atcgcgtaaa ggaaagcatt ttccgtttac aggggacgag
     6241 catcaaatgc agcaatatcc tctacaatgc cgaggaccgg aaacggatga gcgatacgca
     6301 actgctaaag atcctgcagt atgcccgtat cgtgaaggtc gaagatatca gcgccaagga
     6361 ccgcgccgat tataaggaac ggatcaaggc gaacatgaaa gaggtggtcg tcgttgaatt
     6421 gtcggatttc atcgagagga acatcctcgc caaaggatat ttgaactatg acgccgaaaa
     6481 gttgatcgac atcgctaatg cgacggcccg taagatgtat cttctcatcg agaaatggcg
     6541 cggttgggag aaaagcgata tcattcgacg ctcctgccgg ttccttgcga gccgcatccc
     6601 cctttcctgg gaggacaaga atgtctccgg ctccatcaaa tccatagaaa aggcggctca
     6661 ggaattaaag atcaaaggac tgatcggcga ttatcagttg agtcgtgcag gcggcgtcaa
     6721 ggacaccgag atcatctttt tctttgaagg agcgaccgcc tcggcccggg cacaggcgat
     6781 acggaaacgg aacgaggaac agatacagga aacagggcac gaagggatac agatccttga
     6841 tgtgacctat gatgaacggc aatcgaccat attcgactcg aatgggacgg caaaggaccc
     6901 cgatctcctc aatgaaccct cggaacttgc ggcgctcatc gaactggtgc cggcggtcag
     6961 cagaaccaag tccctgcaag cgctcatcgc ccggtatctg aaagaaaagg ggggcgaata
     7021 tgtctcttcc aatatcgagt attgccgaaa gaacgcgcga gacttcggag gcatgttgcc
     7081 cggcgcgctt cagaaggatt tcggtgaagg gatccgtctt gcccgggccg ccgaggaaca
     7141 ggaacgctcc gtgaaaaaaa gagcgcgcga acaagcatcg aacaccgaac aggagagatt
     7201 ggctttcgag gagtggtggc gtggcctcac cgacaaggaa aaagccgagg tcgacatacg
     7261 ggcggccgag atggctgagg cggccggtct ctcgaaaacg gtcatgggct tggccacggt
     7321 ggcgaaccgc gtaaaggtct ataaggaacg aaagaaaaaa gccctctgat ctcaccgata
     7381 actcaaaacg agcgctttac cttacataca tatgtatgta aagagttacc cgccgagata
     7441 ctcgttcatg aacttgttga acaggtccga aggcgttttc ccgtctcgat gacacagcgc
     7501 cttgaaggtt ttaaaaagat tgtgatagag attgagcgtg ataccgcttt tgatatcgtt
     7561 cggaaggacc aaaggcgtct cctgtaacga gggaacaacc gatactaccg gcgttttatc
     7621 ggcggcggaa ggcgtctgcg gcttttcggg aatgggctcc gcggggattt tcggttctgg
     7681 cgttttccgg gtggctttct cttgaggggc cggggccaaa ggttgaggag tcggcttttc
     7741 tttcttcttc gtcccggctt tcgcctttac cttttccgag gatcttggca ccgccgactt
     7801 tttcacggct ttcccttggg gttgctcctc tttccccttc ttaggtttaa gcgccacatc
     7861 ggcggtagcg ccggaaatga atttctccaa caccttgtcc gcattgggcc gcttcaacct
     7921 gtttttatcg aacggcatgg ttcacctccc tgaataactc ctgtatctcc gctgacgctt
     7981 tggcgaattt ctcccctttc atttcggtca ccgaacagcc ttccgcaaag gcctccttga
     8041 aaccgatacg gaaatagatc atggacatga agaggaatag cgaatatttg tcggcatact
     8101 cccccagtat ctcaagaaca tcggtcgtta tcgaaacacc cggctgtacc atattgagaa
     8161 ggatccccgc ctttatgtct ttggcggtcc gaagttcttc cagcatcttg aatacatctt
     8221 cggtgctcca gaggtcgacg ggactcggtg tgaccgggat gatgagcaga tcggaggcga
     8281 taagggcgtt tctgaac
//
LOCUS       DAOQUE010000065         8235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_9632_length_8235_cov_4.395599, whole genome shotgun sequence.
ACCESSION   DAOQUE010000065 DAOQUE010000000
VERSION     DAOQUE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9632_length_8235_cov_4.395599"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PKH10_03160"
     CDS             <1..660
                     /locus_tag="PKH10_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27156.1"
                     /translation="SDGYYDKGFATCLADCTGWDYDYCERICGNHQKDEGEACDGDSL
                     SCAEFNPVLYGGGTAACKEDCSGYDMSTCQSKCPTDNPFCRERGGKYWSDILVIESSA
                     DPIVACQSYGGRLPTISELRSLVQHCPALEPNGGCGVTDSCLAYAACYEGKGCEGCTD
                     SSQPAGYFSAFGDQIALPSISEMPSTFPMIWAIAFSLSNNQVSLYFEYPMSLFQIRCV
                     K"
     gene            780..2072
                     /locus_tag="PKH10_03165"
     CDS             780..2072
                     /locus_tag="PKH10_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNT27157.1"
                     /translation="MTTKLYFKDVTIFDGDAFFRGSLTTDGDRITRIVRNGEIPDGGT
                     PTAAGEAVLIMPGMVNLHTHVPMTLFKGVAEDLPLKDWLEKRIFPLEAKFISREMCRI
                     AGQWGMAEMLLAGVTAFADMYFFEEDIAAAAKEIGIKAAIGGGVINFTTPDGKSPDQM
                     IAYTVELAETYRDDPWIFPCCAPHSPYLTNENCLLTLAEISQKYRIPFHIHMAETRHE
                     QEAYQKQHGKREFERFDELGLLSDRFIAAHSVWVDDKEMDLMASRGVTVAHCPSSNLK
                     LGSGIAPVAAMVERGVNVAVATDGSASNNNLDVLKEAELAAKVQKGTAHDPTVLPAGM
                     VLKMVTANPGRALGNSFGRLIEGGAADIVQVRLDSVGIAPVHDPAAAIVYASSPADIA
                     AVWVNGRQTVKEGKLLTVDRAALGNEMDRLAAAVRRSL"
     gene            2069..4522
                     /locus_tag="PKH10_03170"
     CDS             2069..4522
                     /locus_tag="PKH10_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNT27158.1"
                     /translation="MRGGAPIPVLTRYRDLPYAKILVWFRGGSSVEAPEELGWAHLTE
                     HLLFKPRHAGATLAEFVESLGGSSNAFTSQDAVAVEATVRVEDVDRVLAHLSAVLSLP
                     LTAIARKDLDEERSVVIEELRMYRDEPTENLFNATMRNLYPGHPYGREIIGEEETLRD
                     ATGQTLERFLREKLSVRPFVVVAGGYRGAPAFSLPWRDAATPVTLTPWETARRFTLSH
                     RQKKAYFMAGWRLPPESGETLAAARLIHLIVHGMEGSRFYHELVYEHGTFDNMTVHLL
                     NGFDALSFLHSLACDPSREPARVARWMREWDRVEITREEVAKARETILSDESYAAEGV
                     GAVAETMARGQALYGDPARLERDHFWHLLHLSASELRAFKETHLSWDRAVAGAALPAE
                     NRFSFTTAAHRVAATTAKRAPGLKERPQASVRFRESDRAPFVSLYALKQGGAGGGLPG
                     KPGSLRLFWETLTASAEGLDRTGTDEFLDRHGIILDPIVGNNTSGIRLKMRDSSVREA
                     VDIFARVLRNRLHTEDLAQEKAHTLANLSLKGESPESLLRDHARRVMFAGTAYADPLE
                     GTVGSVAPLTLADLRATRRLCFASGRWGLGVSGAADRSFVEDIAAALPHHRGEATGDR
                     RSATVTLDDRVETVAIPEKDQIHIVRLFRAPGIYDADFETMRLIEQLLTGQRSPYFQA
                     LREEQGLVYHLDIWSMGGLREGLFALYAITSPAHRDRVMDRMTAALAPLRTGALAPDL
                     FAEAKNTLAFDHARSLTRNDFHALNLALEGALGLPRDHYLALPSLVSTLSVERMAACA
                     AAYLTNGIWLVSGGDNERE"
     gene            4509..5297
                     /locus_tag="PKH10_03175"
     CDS             4509..5297
                     /locus_tag="PKH10_03175"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNT27159.1"
                     /translation="MKENSWYQAAVPLDHPLDRYYRIPGFLGSAEIDGRQMLYFEKSD
                     ATARLLKGLDLALISEGDWEERWRDYFQPTPFAGFTVVPPWLKRQGDLIINPGRGFGT
                     GRHETTLLVAETLRDLLSDRSLKSLLDVGTGSGILAIAAKKMRPDLAVTAIDNDPDAV
                     ENAAENLARNGLAGSVRLSLTPLSRFRRPYDIVVANIISGTLIELSSELRKKTLSYLL
                     LSGILLTERDDLLPRLDTAGFRLLSSGVRGEWISLLFQRWHGKR"
     gene            5284..6183
                     /locus_tag="PKH10_03180"
     CDS             5284..6183
                     /locus_tag="PKH10_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT27160.1"
                     /translation="MGNDKLDRDSSTQVLNIQDNTELDFRKDRYILLEIYGRNIGKRR
                     DIEKDGIVVGRAEDCDLPLEDPSSSRRHLRITRQQKDFVVTDLKSTNGTYLNDVRLTE
                     PAVLKNGDRIKVGTTIFKFIWSDDMEADYLDQLYQYTIRDGLTGLYNKKNLLETLDNE
                     FQRCKRYSRQLSLMMMDLDHFKNINDTYGHLVGDRVLVMVAEVLKSYFRTADFIARFG
                     GEEFCVILPETPMSSAHLTAERIRMAVSNIKLDDNGREVTVTISIGIAAFDPAMEKPD
                     DLVALADKLLYKAKENGRNRVES"
     gene            6186..6617
                     /locus_tag="PKH10_03185"
     CDS             6186..6617
                     /locus_tag="PKH10_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNT27161.1"
                     /translation="MLKHLSSLFSPQFIKFCVVGGLGLVTDQTVFFLLSTIVDPASVL
                     VNFVWVVGYLLAVVQNYLINHYWTFKAQTSESRPSGRGLVVFLLVSLTSLIPRFIAYK
                     GVLVLFGVSQITMNLANLCGIVAGTVVNFFGSKFIVFAKKA"
     gene            6617..7369
                     /gene="dapF"
                     /locus_tag="PKH10_03190"
     CDS             6617..7369
                     /gene="dapF"
                     /locus_tag="PKH10_03190"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNT27162.1"
                     /translation="MLFHKYHAAGNDFIITDDPAVIPAQAPALCDRRTGIGADGILLH
                     RPAPDADAEMVIINADGSTAAMCGNGLRCFCRYLLEQRGTAKNSLRILTGAGTIDCLV
                     SRDNGDWSVTLDLGTATTTPSPDGTIVDIGNRHLVVRGGPGADTARDTASRLQEKFRG
                     DINVEVLTGLDERTRTVDLIVNERGAGFTRACGTGGGAVVAALHHDGLVATGDEWTLR
                     FPGGPLRYRRDGRGHIILRGDARFVFSGTTDG"
     gene            7362..>8235
                     /locus_tag="PKH10_03195"
     CDS             7362..>8235
                     /locus_tag="PKH10_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27163.1"
                     /translation="MAKPVEPADIERFLRLATGEEPFPQTEFLSQRLDLFRAVLSELA
                     AGPADPVRDDAVCERLVATLVFGNRFFDLASLHAFLKKCPRPEDGNAHDIYPAFRAAL
                     LRRIRDLFIATLTYHSPELDFLFCETLGEDRQQFLELLGHLFEQQRLYSLRISPSMQK
                     VLYGIADENVRHFVQFSNIHFLAFYLAALRTAAPVAPAHLDKWVSILLSPTTAPAMID
                     KILGALKIHPTVEFLLPLLLLSRDEQRSVVLSVLRTILERRPYLLRDHIPTLAFFLGR
                     AAASHFYDFHRIPQE"
BASE COUNT         1507 a         2824 c         2455 g         1449 t
ORIGIN      
        1 agcgacggct attacgacaa aggcttcgcg acctgcttgg ccgactgcac gggatgggat
       61 tacgactatt gcgaacggat ctgcggcaac caccagaaag atgaggggga ggcctgcgac
      121 ggcgattctc tatcgtgcgc ggagttcaac ccggtcctct acggcggcgg cacggcggcc
      181 tgcaaagagg actgctccgg ctacgatatg agcacctgcc agagcaaatg cccgaccgat
      241 aaccccttct gtcgggaacg cggcggaaag tactggagcg acatccttgt catcgagtct
      301 tccgccgacc cgatcgtcgc ctgccaatcg tatggtggtc gactgccgac catatcggag
      361 ctgcgttcgc tggtccagca ctgtccggcc cttgagccca acggcggatg cggcgtcacc
      421 gattcgtgtc ttgcttacgc cgcctgctac gagggaaaag ggtgcgaggg atgtaccgat
      481 tcctcacaac ctgcaggata tttctccgct ttcggcgatc agatagcgct tccgtcgata
      541 tcggagatgc cctcgacctt tcctatgatc tgggctatag ctttctcttt atcgaacaac
      601 caggtttcgc tgtattttga gtaccccatg agtctttttc aaatccgttg cgtcaagtaa
      661 cgaaatgatc cccgcccgcc atcgcgtgac tttctattga cttgtccgcg catccgctat
      721 aatcggcaga acccgccgtc gccggaggct atggcggaca agcccacacg gaggcaccca
      781 tgaccacgaa gttgtatttc aaggatgtga cgatattcga cggggacgcg ttcttccgcg
      841 ggtcgctgac gaccgacggt gaccgcatca ctcgcatcgt gcgaaacggc gagataccgg
      901 acggcggcac gccgacggcg gcgggcgaag cggtgctcat catgcccggc atggtgaacc
      961 tccacaccca tgtcccgatg acgctcttca aaggggtcgc cgaggacctg ccgctgaaag
     1021 attggcttga aaaaaggata ttcccgctgg aggcgaagtt catctcgcgc gagatgtgcc
     1081 gcatcgccgg tcagtggggc atggccgaga tgctcctcgc gggggtcacc gccttcgccg
     1141 acatgtactt tttcgaggag gatatcgccg ccgccgcaaa ggagatcggc atcaaggcgg
     1201 ccatcggcgg cggggtcatc aacttcacga cccccgacgg caaaagcccc gatcagatga
     1261 tagcgtacac ggtcgaactg gccgaaacat accgggacga tccgtggata tttccctgct
     1321 gtgcccccca ttcgccttat ttgaccaacg agaactgcct gctgacgctt gccgagatct
     1381 cccaaaaata ccgcatcccg ttccacatcc acatggccga gacgcgccac gaacaggagg
     1441 cctaccagaa acagcacggg aaacgcgagt tcgagcggtt cgacgagtta gggctcctgt
     1501 cggaccgctt catcgcggcg cacagtgtct gggtcgacga taaagaaatg gatcttatgg
     1561 cctctcgcgg cgtcacggtc gcccactgcc cgtcgagcaa cctcaaactc ggaagcggca
     1621 tcgcgccggt cgccgcgatg gtggaacgcg gcgtcaatgt cgcggtcgcc accgacggct
     1681 cggcgagtaa caacaacctc gatgtgctca aagaggccga actggcggcg aaggtgcaga
     1741 aagggacggc gcacgacccg accgtccttc cggcgggcat ggtcttgaag atggtgaccg
     1801 ccaaccccgg tcgcgcgctc ggcaactcgt tcgggcggct tatcgaaggg ggcgccgccg
     1861 atatcgtgca ggtgcgtctc gactccgtcg gcatcgcacc ggtccacgat cctgccgcgg
     1921 cgatcgtgta tgcctcctct cccgccgaca tcgcggcggt ctgggtgaac ggccgccaaa
     1981 cggtcaaaga gggaaaactc ctgacggtcg acagggccgc gctcgggaac gagatggacc
     2041 gtctcgccgc cgcagtgcgg agatcgttgt gaggggcggc gcaccgatac cggtgctgac
     2101 ccgctaccgc gatcttccct atgccaagat actggtctgg ttccggggcg gcagttcggt
     2161 cgaagcgcca gaggaactgg gctgggcgca tctcaccgaa cacctgctgt tcaagccgcg
     2221 ccatgcgggg gcgaccctcg ccgagttcgt cgagtcgctc ggcggcagca gcaacgcctt
     2281 cacctcgcag gacgccgtcg cggtcgaggc gacggtccgc gtcgaagatg tcgatcgcgt
     2341 gctcgcccac ctttcggcgg tgctctccct gccgctcacc gcgatagcgc ggaaggatct
     2401 cgacgaagag cgatcggtcg tcatcgagga gttgcggatg taccgcgacg aaccgaccga
     2461 gaacctgttc aacgcgacga tgcgcaacct ctaccccggc catccgtacg gtcgcgagat
     2521 catcggcgaa gaagagaccc tgcgcgacgc cacggggcag acgctcgaac ggttcctgcg
     2581 cgaaaaactt tcggtgcggc ccttcgtcgt cgtcgcgggc ggctatcgcg gcgcgcccgc
     2641 tttttctctg ccatggcgtg acgccgcgac acccgtcacg ctcaccccgt gggaaacggc
     2701 gcgacgcttc acgctgtcgc accgtcagaa gaaggcatac ttcatggccg gctggcggct
     2761 cccgcccgag agcggcgaaa cgctcgccgc agcgcgactc atccatctca tcgtacacgg
     2821 catggaagga agccggttct accatgaact ggtctacgaa cacggcacct tcgacaacat
     2881 gacggtgcac ctgctgaacg gcttcgacgc actctcgttc ctccacagtc tcgcctgcga
     2941 cccgtcgcgc gaaccggcgc gggtcgcccg ctggatgcgc gagtgggacc gggtcgagat
     3001 aacccgcgaa gaggtggcga aggcgcggga gaccatactg agcgatgaaa gttacgccgc
     3061 cgaaggggtc ggcgcggtcg ccgaaacgat ggcgcgcggt caggcgctgt acggcgaccc
     3121 cgcgcggctc gaacgggatc acttctggca cctcctccac ctttcggcga gcgaactgcg
     3181 cgcgttcaaa gagacacacc tttcgtggga ccgcgcggtc gccggggcgg cgcttcccgc
     3241 cgagaaccgg ttctcgttca ccaccgccgc acaccgtgtc gccgcaacga cggcaaaacg
     3301 cgcaccgggg ttgaaggagc ggccgcaggc gagcgtccgc ttccgggaga gcgaccgggc
     3361 cccgttcgtg tcactctacg ccctcaaaca ggggggcgcg ggcggcggac ttcccggtaa
     3421 gccggggtcg ctccgtctct tctgggaaac gctcaccgcg tcggccgaag gtctggaccg
     3481 caccgggacc gacgagttcc tcgaccgcca cggcatcata ctcgacccga tcgtcggcaa
     3541 caacacgagc ggtatccggc tcaagatgcg cgactcctcg gtgcgcgagg cggtcgacat
     3601 cttcgcccgg gtgctccgga accggcttca caccgaggat ctggcgcagg agaaggccca
     3661 cacgctcgcc aatctttcgc ttaaaggaga gtcgcccgaa tcgctcctgc gcgaccatgc
     3721 ccgccgggtg atgttcgccg ggaccgccta cgccgacccg ctcgagggga ccgtggggag
     3781 cgtcgccccg ctcacgctcg ccgacctgcg cgcgacccgg cggctctgct tcgcctcggg
     3841 ccggtgggga ctcggagtga gcggcgcggc cgaccgttcg ttcgtggagg atatcgccgc
     3901 cgcactgccc caccaccgcg gtgaggcgac cggtgaccgc cggtcggcga cggtgacgct
     3961 cgacgaccgc gtcgaaacgg tggcgatccc cgaaaaggac cagatacaca tcgtccgtct
     4021 gttccgcgcc cccggtatct acgacgccga cttcgagacg atgcggctca tcgagcaact
     4081 gctcaccggc cagcggtcgc cctactttca ggcgctccgt gaagagcagg gactcgtcta
     4141 tcacctcgac atctggtcga tgggcggcct gcgcgaaggc ctgttcgccc tgtacgccat
     4201 cacctccccg gcgcaccgcg atcgcgtgat ggaccggatg acggcggcgc tcgccccgct
     4261 caggaccggc gcgctggcgc ccgatctttt cgcggaggcc aagaacacgc tcgccttcga
     4321 ccacgcccgg tcgctcaccc gcaacgattt ccacgcgctc aacctcgcgc tcgaaggggc
     4381 gctcggcctg ccgcgcgacc actatctcgc tctgccctcc ctcgtttcca cgctctcggt
     4441 cgaacggatg gccgcctgcg ccgccgcgta tctcaccaac gggatatggc tcgtgtcggg
     4501 aggagataat gaaagagaat agttggtacc aagccgccgt cccgctcgat cacccgctcg
     4561 atcgctacta ccggataccg gggttcctcg gaagcgctga gatcgacggc aggcagatgc
     4621 tctatttcga gaagagcgac gcgacggcgc ggcttctcaa aggactcgac ctcgccctca
     4681 tcagcgaggg ggactgggag gagcgatggc gcgactattt ccaaccgacc cctttcgccg
     4741 ggttcaccgt cgtgccaccg tggcttaaac gacagggcga tctcatcatc aaccccggtc
     4801 gcggcttcgg caccggccgc cacgaaacaa cgctcctcgt cgccgagacg ctccgcgacc
     4861 tgctctccga ccgttcgctg aagagcctgc tcgatgtcgg caccggctcg gggatactcg
     4921 ccatcgccgc gaaaaagatg cgccccgacc tcgcggtcac cgcgatagac aacgaccccg
     4981 acgcggtcga gaacgccgcc gagaacctcg cacgcaatgg acttgccggt tcggtgcggc
     5041 tgtcgctcac gcccctttcc cgcttccgca ggccttacga catcgtggtc gccaacatca
     5101 tatcgggcac actgatagaa ctttcctcgg aattgcgtaa aaaaaccttg agttatcttt
     5161 tgctatcggg tattctgctc accgaacggg acgatctcct tccgcggctg gataccgccg
     5221 gtttccgcct gttgtcctcc ggcgtccgcg gggaatggat atctctcctg ttccagaggt
     5281 ggcatgggaa acgataaact cgaccgcgac tcatcgacgc aggtcctgaa cattcaggac
     5341 aacaccgaac tcgacttccg caaggaccgc tacatcctgc tcgagatcta cgggcgcaac
     5401 atcggcaagc ggcgcgacat cgaaaaggac ggcatcgtcg tcggccgcgc cgaggactgc
     5461 gatctcccgc tcgaggaccc gagttcctcg cggcgtcacc tgcggatcac gcgccagcag
     5521 aaggatttcg tggtcaccga cctcaagagc accaacggca cctacctcaa cgatgtccgg
     5581 ctgaccgagc cggccgtgct caagaacggc gaccgcatca aggtcggcac caccatattc
     5641 aagttcatct ggtccgacga tatggaggcc gactacctcg accagttgta ccagtatacc
     5701 atccgcgacg ggctcaccgg cctctacaac aagaagaacc tgctcgagac gcttgacaac
     5761 gagttccagc gctgcaaacg ctacagccgc caactgtcgc tcatgatgat ggatctcgac
     5821 catttcaaga acatcaacga cacctacggc catctcgtcg gcgaccgggt gctcgtgatg
     5881 gtcgccgagg tgctcaagag ttatttccgc accgccgact tcatcgcgcg cttcggcggc
     5941 gaggagttct gcgtgatcct gcccgaaacg ccgatgtcgt cggcccacct gaccgccgag
     6001 cggatacgga tggcggtgtc gaacatcaaa ctcgacgaca acggtcgcga ggtgacggtg
     6061 accatcagta tcggcatcgc cgcgttcgac ccggcgatgg aaaagcccga cgatctggtc
     6121 gcgctcgccg acaaactgct gtacaaggcc aaagagaacg gtcgcaaccg cgtcgagagt
     6181 tgaccgtgct gaaacacctc tcgtcgcttt tctcccctca gttcatcaag ttctgcgtgg
     6241 tcggcgggct cgggctcgtc accgatcaga ccgtcttttt cctgctgagc accatcgtcg
     6301 atccggcgag cgtgctcgtc aacttcgtct gggtcgtcgg ctacctgctc gcggtcgtgc
     6361 agaactacct catcaaccac tactggacct tcaaggcgca gacgagcgag agccgcccgt
     6421 cgggcagggg gctcgtcgtg ttcctgctcg tgagtctcac ctctctgata ccgcgcttca
     6481 tcgcctacaa gggggtgctt gtgctgttcg gcgtgtcgca gatcaccatg aacctcgcca
     6541 acctctgcgg catcgtcgcg gggaccgtgg tgaatttctt cgggtccaaa ttcatcgtgt
     6601 tcgcgaagaa agcctgatgc tgttccataa ataccacgcc gccggcaacg acttcatcat
     6661 caccgacgac ccggcggtga tccccgcgca ggccccggcc ctgtgcgacc gtcgcaccgg
     6721 catcggcgcc gacggcatac tcctccaccg cccggccccc gacgccgacg ccgagatggt
     6781 catcatcaac gccgacggct ccaccgccgc gatgtgcggc aacggcctgc gctgtttctg
     6841 ccgctatctc ctcgaacagc gggggacagc gaagaactcc ctgcgtatcc tgaccggcgc
     6901 cggcacgatc gactgcctcg tgtcacgcga caacggcgat tggagcgtca cgctggacct
     6961 cggcaccgcc acgacgacgc cctcccccga cgggaccatc gtcgacatcg gcaaccgcca
     7021 tctcgtcgtg cgcggcggcc ccggcgccga cacggctcgc gacaccgcca gccgtctgca
     7081 ggaaaagttc aggggcgaca tcaatgtcga ggtgttgacc ggtctcgacg agcgcacgcg
     7141 caccgtcgac ctcatcgtca acgagcgcgg cgcgggcttc acccgcgcct gcggcaccgg
     7201 cggcggcgcg gtcgtcgccg cgctccacca cgacggcctc gtcgccaccg gcgacgaatg
     7261 gacgctgcgg ttccccgggg gccctctccg ctaccgacgc gacggacgcg ggcatatcat
     7321 cctgcgcggt gacgcgcggt tcgtcttctc cgggacgacc gatggctaag ccggtcgaac
     7381 ccgccgacat cgaacggttc ctgcgtctgg cgaccgggga ggagccgttc ccgcagaccg
     7441 aattcctttc tcagcgtctc gacctgttcc gcgcggtcct gtcggaactc gcggccggcc
     7501 ccgccgatcc ggtccgtgac gacgcggtat gcgaacggct ggtcgccacg ctcgtgttcg
     7561 gcaaccggtt cttcgacctt gcctcgctcc acgcgttcct gaaaaaatgc ccgcgccctg
     7621 aggacggcaa cgctcacgat atctacccgg cgttccgggc ggcgctcctg cggcgcatcc
     7681 gcgacctgtt catcgcgacg ctgacctacc attcgcccga actcgatttc ctgttctgcg
     7741 aaacgctcgg cgaggaccgt cagcagttcc tcgaactctt gggacacctg ttcgagcagc
     7801 aacggctcta ctccctgcgc atatcgccga gcatgcagaa ggtcctttac ggcatcgccg
     7861 acgagaatgt ccgccatttc gtgcagttct cgaacatcca tttcctcgcc ttttacctcg
     7921 ccgcgctccg gaccgccgcg cccgtcgccc cggcacacct cgacaagtgg gtgtcgatcc
     7981 tgctgtcgcc gaccaccgcc cccgcgatga tcgacaagat actcggcgcg ctgaagatac
     8041 atccgaccgt cgagttcctc ctgccgcttc tccttctttc gcgtgacgaa cagcggagcg
     8101 tcgtgctgtc ggtcctgcgc accatcctcg agcggcgccc ttatctgttg cgcgaccata
     8161 tcccgactct cgccttcttc ctcggacgcg ccgctgcgtc gcatttttac gattttcacc
     8221 ggataccaca ggagc
//
LOCUS       DAOQUE010000066         8215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_9664_length_8215_cov_4.171691, whole genome shotgun sequence.
ACCESSION   DAOQUE010000066 DAOQUE010000000
VERSION     DAOQUE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9664_length_8215_cov_4.171691"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..868)
                     /locus_tag="PKH10_03200"
     CDS             complement(23..868)
                     /locus_tag="PKH10_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27164.1"
                     /translation="MRRCPMSFLVCSFLLMLGCGPENTVSPDHDDAVSDTASAADNDE
                     AIPDEGEVVYPDSSDDMIPDEQPEEVVDGAVDEMSEEMPDGTVDEAVDEIPDEVIDEQ
                     ADIDQGPQTYPAETPTSNKEGDIAANLTFYDHLGNVRKLEEFYLTKKVLWLAFLAYDC
                     PYSNQLKIDLPSVYKSEYVGKGFEIILIENGLLSGPQSSKEPAKIAALRDTMISDYGA
                     NADFVYGYLTNTEQMTFYKYLNQGYPVSVIIDADTMRIIEHLEGWDSSLTTDMEWAVS
                     TNLDE"
     gene            complement(896..3703)
                     /locus_tag="PKH10_03205"
     CDS             complement(896..3703)
                     /locus_tag="PKH10_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27165.1"
                     /translation="MRKILTCALLALGLVLIGGCDDGKKKDDTGVTGCGNDKIETGEL
                     CDGSSLAGITCEKLGFGTGPLFCEDDCLAFDTHMCGASLTCGDNIKNGVDVCDGSDLG
                     GQSCQTQGFSDGQLACLANCAGYDTSSCTGQILCGNNTVEGTEVCDGTQLNGATCESE
                     GFGPGKIACKGDCSGYDTTGCGEECQPQCGTRVCGPDPLCNESCGDCGAFEICSEEGQ
                     CVKTCDLDEITANTVVNIDLQTATVTGQISLNGAVMPDNTLNEYYERGHVIFFHKKSG
                     AALSLPVGITGIAQFSGTLFAGIYDIRYTPNTLDYQNVTPETQYVLAEEVDIAGAWQH
                     NYNLPTVTVSGTITKNGAQVPDNTLNEYYDRGSVRFTDKKSGDSFSVPIGITGSATFL
                     ATLFAGDYRVTLVPNGADYQNVLPDQNLILSENETLSADRVLSYDVGTASLSGTVTLN
                     GAQMPDNVLNEYYDRGYVRFTDIASGSTLSFPLGITGPANYTGEVFKGTYRVLLVPNG
                     ADYQDVLPDLMHELEQGVTFGGVSMRDFNVNTAEVKGVITLDGGQMPPNTLNEYNERG
                     YVRFTDTVSGGSISFPIGITGMAEYVADIYTGTYKVLLTPNGTDYQDVLPDMYKKLDD
                     ALSVTGDMVKHYPVETVSFTATVTLNGAQMPDNTLSQYNERGYLRFSNLGSGDSYSFP
                     LGVSGPAAVTVKVWKDDYQVILVPEGDDYQDVLPNLKLVLEKRRTITDGAPVSWNAST
                     VGVGGKVTLNGATMPNNTLNEYNDRGWLRFINKRSSDYLPASLGILGEATYSITLWAG
                     PYDISLAPNGDDYQNVLPDLLIPLRTGCYELGADCQSDKGDITGVWELIAEDPNWGVW
                     MFNFTQNGATVTGSGSNNFGQSAAVQNGTRSGDTISFKIQPYGEVAVKGTLLNGCAMT
                     GTFEDLSYGQGIIKWVGERIE"
     gene            complement(3791..5125)
                     /locus_tag="PKH10_03210"
     CDS             complement(3791..5125)
                     /locus_tag="PKH10_03210"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNT27166.1"
                     /translation="MKKVFGMFAVILFSGALLAAGTMQDLEREVTLISEKAQKSVAFI
                     KVVKQGRGGMGMDPFWDMLRDFGFRGQNPRFNPQQEGLGSGFVMNAKEGLLVTNNHVI
                     EGADKIEVTINKKTFPAKLVGTDPKTDIGLLRIEKFAAGDIESLPLADSDAVKVGSFA
                     IAVGNPFGLSQSVSFGIVSAKGRSNVNITEYEDFIQTDAAINPGNSGGPLLNIRGEVI
                     GMNSAIFSKSGGYMGIGFAVPSNMIKNITDQLRGGKKIKRAMMGVNIQDLTPELAKHL
                     GLPENQLGVLLSSVAKGSPAEKAGIRKGDVVVSFDGKPVESSAQLRNLVAFSAFDRKI
                     PVVVSRDGKQVTLALVLSERFEFEQGGDDEAQANDFGFGIVEKNGKVLVDRIEPQSIA
                     AMAGLREGDIIEEINRKPVRNQKEFNAAMGQAKSVLLLIDRKGRQFFVVINR"
     gene            complement(5196..5534)
                     /locus_tag="PKH10_03215"
     CDS             complement(5196..5534)
                     /locus_tag="PKH10_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNT27167.1"
                     /translation="MNDCIFCRIARGDIPAKKVIENDRFVVFHDISPLYKVHLLIVPK
                     RHIASVNDVDAASGEALAEIFGTAREAAEKVGVAGNGYRLTVNTGRDAGQVVHHFHMH
                     LLAGEPLRPL"
     gene            complement(5551..6249)
                     /locus_tag="PKH10_03220"
     CDS             complement(5551..6249)
                     /locus_tag="PKH10_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27168.1"
                     /translation="MTRCLHCGYENNNPSCCAGCGRGLTRPIAGPVCCGPDQAVSLVD
                     EMRQAVERVFPSDTSSSEEPFMARPAAAGIDVSRIAAESGLFIVGEARYEPLTGKETA
                     RGFFLETAVVIVVSLLLAFISKVWGHYGVVAATKVYLVSFMALSSATWLLFPYLFGSS
                     PLVALLFRFSLIRQERKSAQGDAAATLLLWFVSLSYSLIPLLFAEYLYLMVARDHYQP
                     LSFQLSDIRYMRAC"
     gene            complement(6246..7175)
                     /locus_tag="PKH10_03225"
     CDS             complement(6246..7175)
                     /locus_tag="PKH10_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27169.1"
                     /translation="MKGPFLIAGSLIALSFLHAEPSAQPGRAVPIAPQAAPLSAEPSV
                     PADLDARLKGAGLNWLQVRVNQSVARIIREKKVSLEELNEYLYLMAVPNFAYRGDAGE
                     ERLGNDMMGLLGTIKTGEDVEIMEMQWSEMAGMGLPFRKRIIDALLFPPDYFKDAIDL
                     TFFVNMHVLTARIGAALSVRPGIKPLYNSQQKFYRLKKESLQFIYIVNIPNVAYEMVY
                     TGEPLENVIKKYLQEVDLLFPLLERYEKRILSDKTLSYDGRYVKGKNVFIDLWFLHER
                     IKGSAGEAEALLEKALKHQPVEVSGSEKKGNSR"
     gene            complement(7172..7597)
                     /locus_tag="PKH10_03230"
     CDS             complement(7172..7597)
                     /locus_tag="PKH10_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNT27170.1"
                     /translation="MRCGRCLAACPFYRETRREEYSPRGRVVLTEKTGKPDTVRCFAC
                     HNCGTVCPYELSPLDRPGQKAGPDLSPIEATLFALVFYGVEPSSDHAADPRIRAWMAK
                     KGLIRDDAALLRAQRFPNGELFHPFLVMPGERDKEGRSI"
     gene            complement(7657..>8215)
                     /locus_tag="PKH10_03235"
     CDS             complement(7657..>8215)
                     /locus_tag="PKH10_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNT27171.1"
                     /translation="AVSVTDLFSTEMVNTGMFEVVERMQVAKILNEMGFQQTGVTDSS
                     AAVEVGKMLNTKLVVIGTIGKLGQKLVLTAKIVEVEKGKILFAEKEMANGEDDLVGAC
                     EELANKLVFNITGVKIKNRKTSRVQGTSAAPAPQTTAQPAPAPQPAPAGTKADPKKKS
                     DKKPVKEKDPVRKHSKEAMNELDDM"
BASE COUNT         1603 a         2504 c         2342 g         1766 t
ORIGIN      
        1 gggggcaggg gtgtctctgt cgtcattcat cgagattggt cgataccgcc cactccatat
       61 cagtcgtcag agaagagtcc cacccttcaa ggtgttcgat tatcctcatg gtgtcggcat
      121 ctatgatgac actgacggga tacccctgat taagatattt atagaaggtc atctgttcgg
      181 tgttggtcag atagccatag acgaaatcgg cgttggcgcc atagtccgag atcatcgtgt
      241 cgcgcagagc ggctatcttc gccggttctt tcgaagattg cggccccgag agcagaccgt
      301 tctctatcag gatgatctcg aacccttttc ccacatactc gcttttatag accgagggaa
      361 ggtctatttt gagttgattg ctgtaagggc aatcgtaggc gaggaatgcc agccacagga
      421 cctttttcgt caggtagaat tcctcgagtt tccgtacgtt cccgagatgg tcgtagaagg
      481 tcagattggc cgcgatatca ccctccttgt tcgatgtcgg cgtttcggcc ggataggttt
      541 gcggcccctg atcgatatcg gcttgttcgt caatcacctc gtcgggtatt tcatcgaccg
      601 cctcgtcgac cgttccgtcg ggcatctctt cggacatctc gtcaacggca ccgtcaacga
      661 cctcctccgg ctgttcatcc gggatcatgt catccgaact gtccggatag acgacttccc
      721 cttcatcggg gatcgcctca tcgttatcgg cggctgatgc cgtatcggac acggcgtcgt
      781 catggtctgg cgagaccgta ttctcaggcc cgcaccccaa catgagcagg aacgagcaca
      841 ccaaaaaaga catcggacag cgtcgcatcg atctctcctt acttttttta ctggttcact
      901 ctatccgctc gccgacccac ttgatgatgc cctgcccgta ggagagatcc tcgaaggtac
      961 cggtcatcgc gcaaccgttc agcagggtcc ccttcaccgc cacctcgcca tagggctgta
     1021 tcttgaagga tatcgtgtca ccgctccggg tgccgttctg aacagcggcg ctctgcccga
     1081 agttgttcga gccggaaccg gtcaccgtcg caccattttg cgtaaagttg aacatccaga
     1141 cgccccagtt cgggtcttca gcgatcagtt cccagacgcc ggtgatgtca cccttgtcgc
     1201 tctggcagtc ggcgcccagt tcgtaacagc cggtcctgag cggtatcagc agatcgggca
     1261 ggacattctg ataatcatcc ccgttgggag ccaaagagat gtcgtaggga cccgcccata
     1321 aggttatcga gtaggtggct tccccgagga tccccagcga ggccggaaga tagtcggagg
     1381 aacgcttatt gatgaagcgc agccaaccgc ggtcgttata ctcgttcagc gtattgttcg
     1441 gcatcgtcgc gccgttcaag gtcaccttgc cccccactcc gaccgttgag gcattccacg
     1501 ataccggcgc cccgtcggta atcgtccgcc gcttttccag caccagtttc aggttgggca
     1561 ggacatcctg atagtcgtcc ccttcaggaa ctaggatgac ctgatagtcg tccttccaca
     1621 ccttcaccgt caccgccgcc gggcctgata cgcccagcgg gaacgaataa ctgtcgccgc
     1681 ttccgaggtt cgagaaccgc aggtagccac gctcgttgta ctgactcagg gtgttgtcgg
     1741 gcatctgcgc accgttcagc gtcacggtag cggtgaagga taccgtttcg accgggtagt
     1801 gtttgaccat atcaccagtc accgacagcg catcgtcgag tttcttatac atgtcgggca
     1861 ggacatcctg ataatcggtg ccgttcggcg taaggagaac tttgtaggta ccggtgtaga
     1921 tgtccgcgac atactccgcc atgccggtta tcccgatcgg gaacgagatg ctcccgccgc
     1981 tcacggtatc ggtgaaacgg acatatcccc gctcgttata ttcgttcagc gtattcggcg
     2041 gcatctgccc gccgtcgagc gtgatgaccc ccttcacctc ggcggtgttc acattgaaat
     2101 cgcgcatcga gacgccgccg aaggtgaccc cctgctccag ttcgtgcatc agatcgggca
     2161 ggacatcttg ataatcggca ccgttgggaa caaggagaac gcggtaggtc cccttgaaca
     2221 cctccccggt atagttcgcc ggcccggtga tgccgagcgg gaaggaaagc gtggaacccg
     2281 aagcgatatc ggtgaatctg acatatccgc ggtcatagta ctcgttcagt acattgtcgg
     2341 gcatctgggc gccgttcaat gtcacggtcc ccgaaagcga agcggtaccg acatcgtagg
     2401 aaagcacccg gtcggcgctc agcgtctcgt tctcgctcag gatgaggttc tgatcgggca
     2461 ggacattctg atagtcggcg ccgttcggca cgagcgtgac gcgatagtca ccggcgaaca
     2521 gggtggcaag gaaggtggcg gagcccgtga tacctatcgg caccgagaaa gagtcgccgc
     2581 tttttttgtc ggtgaatctg acagagccgc ggtcgtagta ctcgttcagc gtattgtcgg
     2641 gcacctgcgc gccgttcttg gtgatggtcc cgcttaccgt gacggtcggc aggttataat
     2701 tgtgttgcca tgcgccggcg atatcgacct cctcggcaag cacatattgg gtctccgggg
     2761 tcacattctg gtagtcaagg gtattaggag tgtagcggat gtcatagata ccggcaaaaa
     2821 gtgtcccgct gaactgggcg ataccggtga tgccgacagg aagagacaag gcagctccgc
     2881 tttttttatg gaaaaaaatg acatggccgc gttcgtagta ttcgttcagc gtgttgtcgg
     2941 gcatcaccgc tccgttcagc gatatctggc cggtgacggt cgcggtctgc aggtcgatgt
     3001 tgacgacggt attggccgtt atttcatcga ggtcgcaggt cttgacgcac tgcccctcct
     3061 cgctgcatat ctcgaaagcg ccgcagtcgc cgcacgattc attgcagagc ggatcggggc
     3121 cgcagacccg ggtcccgcac tggggctgac actcttcgcc acagccggtc gtgtcgtagc
     3181 cggaacagtc tcctttgcag gcgatcttcc cgggaccgaa tccctctgac tcgcaggtcg
     3241 cgccgttcag ttgcgtcccg tcgcagacct cggtcccctc gacggtattg ttcccgcaga
     3301 gtatctggcc ggtgcaggac gaggtgtcgt agccagcaca gttggccaga caggcgagct
     3361 gcccgtcaga gaagccctgc gtctggcaac tctgcccgcc gagatcgctc ccgtcgcaga
     3421 catcgacgcc gttcttgatg ttgtccccgc aggtcagcga ggcaccgcac atatgggtgt
     3481 cgaatgcaag gcagtcgtcc tcgcaaaaga gcggtcccgt cccgaacccc agtttctcgc
     3541 aggtaatccc ggcaagactc gaaccgtcgc agagttctcc cgtctctatc ttgtcgttgc
     3601 cacagccggt cacgccggtg tcgtccttct tcttcccgtc gtcacagccg ccgatgagga
     3661 cgagccccag cgccaacagg gcgcatgtca ggattttccg catgatactc cctccaaaag
     3721 atcggtaaaa gaccccgaac ggaggaaggt taccctaaaa cagggaagat ttcaagagaa
     3781 gaccgccgtg ttagcggttt atgacgacga agaactgtcg ccccttgcgg tcgataagga
     3841 ggaggaccga cttcgcctgg cccatggccg cgttgaattc cttctggtta cggaccggct
     3901 tgcgattgat ctcctcgatg atgtcgccct cgcgcaaccc cgccatcgcc gcgatcgact
     3961 ggggttctat ccgatcgacc agcaccttgc cgttcttttc aacgatccca aatccgaaat
     4021 cgttggcctg cgcctcatcg tcaccgccct gctcgaactc gaatctctcg gaaagaacga
     4081 gcgcgagggt cacctgtttc ccgtcacggg aaacgacgac cggtatcttg cggtcaaagg
     4141 cgctgaaggc gacgagattg cgcagttgcg ccgacgactc gaccggcttg ccgtcgaacg
     4201 acaccaccac atcccccttc cttatgcccg ccttctcggc ggggcttccc ttcgcgaccg
     4261 acgagagcag aacgcccaac tggttttccg gcaagcccag atgcttggcg agttcagggg
     4321 tgaggtcctg tatgttgacc cccatcatcg cccttttgat cttcttgccg ccgcgcaact
     4381 ggtcggtgat gttctttatc atgttcgacg gcaccgcgaa cccgataccc atgtacccgc
     4441 ccgatttcga gaatatcgcg ctgttcatgc cgatgacctc gcctctgatg ttgaggagcg
     4501 gcccgcccga gttcccgggg tttatcgcgg cgtcggtctg gatgaaatcc tcgtactcgg
     4561 tgatgttaac attcgaccgg ccctttgccg acacgatgcc gaacgacacc gactgcgaca
     4621 ggccgaacgg attcccgacg gcaatggcga aactccccac cttgaccgcg tcggaatcgg
     4681 cgagcggcaa cgattcgatg tcgccggcgg cgaatttctc gatgcgcaac agaccgatgt
     4741 cggtcttcgg gtcggtgccc acgagtttag ccgggaaggt ctttttgttt atggtcactt
     4801 ctatcttgtc ggccccttcg atcacatggt tattggtgac gagcaacccc tccttcgcat
     4861 tcatcacgaa cccgctgccc aagccctcct gttgggggtt gaaccggggg ttctggccac
     4921 ggaagccgaa atcgcggagc atgtcccaga agggatccat ccccatgccg ccgcgtccct
     4981 gcttgaccac cttgatgaag gcaacgctct tctgcgcttt ttcgctgata agggtcacct
     5041 ccctttcgag gtcctgcatc gtaccggccg ccagaagcgc accggaaaag aggatgaccg
     5101 cgaacatgcc aaataccttt ttcatctttg acctctctat cctcacatga tcttcactta
     5161 caacacggag catcccgccc ttattccccg ccccgtcaga gaggccgcag aggctctcca
     5221 gcaagcagat gcatgtggaa atggtgcaca acctgcccgg cgtcgcgccc ggtgttgacc
     5281 gtgagtcgat atccgttccc ggccaccccg accttttcgg cagcctcgcg ggcggtcccg
     5341 aatatctccg caagggcctc tccggaggcg gcatcgacat cattgaccga ggcgatgtgc
     5401 cgcttgggaa cgatgagcag atggaccttg tagagcgggc tgatgtcgtg aaagacgacg
     5461 aaccggtcgt tctcgatgac ctttttcgcc gggatatcgc cccgcgctat gcggcagaag
     5521 atacaatcgt tcatgcccga cacctcctcg ttaacaggct ctcatatagc ggatatcgct
     5581 cagttggaac gaaagcggct gatagtggtc ccgcgccacc atgaggtaga ggtattcggc
     5641 gaacagaagc gggataagcg aataggagag cgatacgaac cagaggagga gggtcgccgc
     5701 ggcgtctccc tgtgcgcttt tccgttcctg ccgtatcagc gaaaagcgga acaggagcgc
     5761 caccagcgga gaagagccga acaggtacgg gaaaaggagc caggtggcgg aggagagcgc
     5821 catgaaggat accagataga ccttggtcgc cgccacgacc ccatagtgcc cccagacctt
     5881 cgagatgaag gcgaggagca gggagacgac gatgacgacc gccgtttcga ggaagaagcc
     5941 tcgggcggtc tctttaccgg tcaggggctc gtagcgcgcc tcaccgacga tgaacagacc
     6001 gctttccgcc gcgatacgac tcacatcgat gccggcggcg gcggggcggg ccatgaacgg
     6061 ttcctcgctc gaggaggtgt ccgaggggaa cacccgttcg accgcctgcc gcatctcgtc
     6121 gaccagagag acggcctgat cgggtccgca gcacacaggc cccgcgatcg gtcgggtcag
     6181 gccgcgaccg cacccggcgc aacaggaagg gttgttattc tcgtagccgc aatggaggca
     6241 ccgggtcatc gggaattccc tttcttctca gaaccgctca cctcgaccgg ttggtgcttc
     6301 agcgcttttt ccaacagcgc ctccgcctct ccggcggacc ccttgatcct ctcgtgaagg
     6361 aaccagaggt cgataaagac attcttgcct ttcacatagc gcccgtcgta ggaaagcgtc
     6421 ttgtcggaga ggatgcgttt ctcgtaccgt tccaagagcg ggaacaggag atccacctcc
     6481 tgcagatact ttttgatcac attctcaagc ggctcgccgg tatagaccat ctcataggcg
     6541 acattgggga tgttgacgat atagatgaac tggagcgatt cctttttgag gcgatagaac
     6601 ttctgctggc tgttatagag cggcttgatc ccgggccgca ccgaaagcgc cgcgccgatc
     6661 cgggccgtca ggacatgcat attgacgaag aaggtgagat ctatcgcgtc cttgaaatag
     6721 tcggggggga aaaggagcgc atcgatgatc ctcttacgga acgggagccc catgccggcc
     6781 atctcggacc actgcatttc catgatctcg acatcctctc ccgtctttat cgtccccaag
     6841 aggcccatca tgtcgttgcc gagccgctcc tctcccgcat cgccgcgata ggcgaaattg
     6901 ggcaccgcca tgagatagag gtactcgttg agttcctcca gcgacacctt tttctcgcgg
     6961 atgatacgcg cgaccgactg attgacgcgg acctgcagcc agttgagccc cgcccccttc
     7021 aagcgggcat caagatcggc gggcaccgac ggctcggccg aaagcggcgc ggcctgcggg
     7081 gcgatcggca cggcccggcc cggttgggcc gacggttcgg catggaggaa ggagagcgcg
     7141 atcagcgatc cggcgatcaa gaacggtcct ttcatatcga tctcccttct ttatctctct
     7201 ctccgggcat cacaaggaac gggtggaaga gttccccgtt cgggaagcgt tgcgcccgaa
     7261 ggagcgccgc atcgtcccgt ataagcccct ttttcgccat ccacgcccgt atccgggggt
     7321 ccgccgcatg gtcgctcgaa ggttcaacgc cataaaaaac aagagcgaac aaggtcgcct
     7381 cgatcgggga aagatccggc cccgccttct ggcccggacg atcgagcggc gaaagttcgt
     7441 agggacacac cgtaccgcag ttatggcagg cgaaacatct caccgtgtct ggcttccccg
     7501 tcttttcggt cagcacgacc cgtccgcggg gagaatactc ttcccgcctc gtctcgcgat
     7561 agaatggaca cgccgcaaga catcgaccac accgcacaca gcgtttggcg accggttcgt
     7621 cggttctggg gggttgtacg gcggacacag acaggatcac atatcgtcga gttcgttcat
     7681 cgcctctttc gaatgcttgc ggaccgggtc cttttccttc accggcttct tgtccgattt
     7741 ctttttcggg tccgccttgg tccccgccgg agctggttgc ggcgcgggag ccggctgggc
     7801 cgtggtctgc ggggcgggcg ccgcggaggt cccctgcacc cgggaggtct tacggttctt
     7861 tatcttgacg ccggtgatat tgaacaccag tttattggcc agttcctcgc aggcgccgac
     7921 caggtcgtcc tctccattcg ccatctcttt ttcggcgaaa agtatcttgc ctttctcaac
     7981 ctcgacgatc ttggcggtca ggaccagttt ctggcccaat ttgccgatgg tgccgatgac
     8041 gacgagtttg gtattgagca tcttaccgac ctcgaccgcg gccgaactgt cggtcacgcc
     8101 ggtctgctgg aaccccatct cattgaggat cttcgccacc tgcatccgct cgacgacctc
     8161 gaacatcccg gtgttcacca tctccgtgga gaacaggtcg gtgaccgaga ccgca
//
LOCUS       DAOQUE010000067         8137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_9771_length_8137_cov_4.384063, whole genome shotgun sequence.
ACCESSION   DAOQUE010000067 DAOQUE010000000
VERSION     DAOQUE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8137
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9771_length_8137_cov_4.384063"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PKH10_03240"
     CDS             <1..1125
                     /locus_tag="PKH10_03240"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT27172.1"
                     /translation="GNGILDTTPTPETCDGGMKACPEINGIYMFGMAPCIATCDGYDE
                     NYCKMSADCEGYPDYLSGADFNLCYFKGNYVPDTAQGQCYDNSAEMGAGCPAPGADFF
                     GQDAQYGGSGKYISMVVTDVVGDDYTGLQWHQMVPQLYSDCNSEMYCTWAQAVTYCEN
                     SMSGGYSDWRLPTDKELESIIEYSGIVPMVPEGVFTMFDAEYFWSSTENVANPTMSAW
                     ATYFAIGTSQPAPKTSQYKAICVRGTFFPTDTKTFTHEWRGGDEVYLDDQTGLMWTGD
                     YWYSDVVPVSDWTEALAYCETGEFAGYTDWRLPSINELGTTYDRTWATPPADFPGFSS
                     YAFWSSTSRPDAPTAAYYLTSGGSTGSQVKTLSGAVRCVR"
     gene            complement(1129..1968)
                     /gene="folE2"
                     /locus_tag="PKH10_03245"
     CDS             complement(1129..1968)
                     /gene="folE2"
                     /locus_tag="PKH10_03245"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNT27173.1"
                     /translation="MKDIQNQRDDRGLPVDMVGVSAIRYPITVKDKLRGMQETVAAVS
                     MSVELLPEFKGTHMSRFIEILNRYRGEITMGNIRDILKETRESLGSPKAMMSLEFPYF
                     IEKKSPVSEARSLFPVDVLFDGYGDGNGTSFILGIRVPVTTLCPCSQEISEFGAHNQR
                     SYITAYLSFDHFIWLEDIVDRLEDCASSEIYPLLKRPDEKYVTEKAFTRPRFAEDIVR
                     GAAELLLAEPHIHWFMAEATHLESIHTHNAYARIERFRDKRPILTAHLQVFKGFRQRT
                     GTY"
     gene            2103..3158
                     /locus_tag="PKH10_03250"
     CDS             2103..3158
                     /locus_tag="PKH10_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27174.1"
                     /translation="MKQMFITFAVLLLCVSCGNTIEMPTDVESDAGSDVGPDTEAEDD
                     AAIVVDEDVVKVPPARVMVQLTWKEGLKARAELDRPEGVAVDLDLHLIKKKGMDACGN
                     EVGADGRMCTVMMTPAGTTIESSTHDDCYWHDVGENAVYYDCSGCEGECRTIAWHAAF
                     VLDNRWGGDDYADPEVVILGPIADEEPKDGFPDVHPFNDEYLIVVNYNNCRDLTEPWD
                     GPQERCKEGGAYYNVHAKVEIFVDDKVAPQAGTQDDKSLSRTEFVIRPDEWIVIGAFI
                     WDGPLVSAVSGWPGDAIVRDVPVSHEKVCRFNSNRCRDLPIWDVAAYEEWVTAPDVPQ
                     DFFASGDGECYDYGERE"
     gene            3171..5252
                     /locus_tag="PKH10_03255"
     CDS             3171..5252
                     /locus_tag="PKH10_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27175.1"
                     /translation="MKNFLVTVSVMFLCLYGCKEVTSPPLTVAEPASWHVGPVVSEAA
                     ADGHYTAGDGTDLPFMRRDFVIRNTGKGAALRIYSINFLSPEGTVLSDALRSALFSIE
                     RSTVSDAHGTVVAGFMPVEENHFENRYPDGTAIVAPLCPLDSEGKCKKGFDAANHNTA
                     LTIRVTYRGLTTEQYAALAVDKLKSDGTYYVELCVNDYANPIESATCTQGNSFRIPIY
                     RSPQTPPKPFIGLEYSSPIGGYKDHRVIKDWVNMEVNLSCPRKEGADEMTCGKIISDT
                     VVSDETITAAEFKEKCCLADWKDWKDRYCIAYRWEMIETPTPLAPETRIGFGNIPGGG
                     EGTWYPACGEFDPTLASFKGHMITPTPEGKYYKVSIQAMTIDKQTQLESDIVEKIDTP
                     NIISAARVMVQLTWKEGLKTRAELDRPEGVAVDLDLHLIKKKGMDACGNEVGADGLMC
                     TVMSRPGLPIDTPTHDDCHWDDLGLNGEYLFTDCNGEFKTIGWHAVYDLDNRWGGGNY
                     ANPEIINLGSVIDQKNNSTGVHGKDGVLDVTPFDDEYLVMVQYNHCQSLQPGYDDAPC
                     QDGGAEYNVHAKVEIFVDGKLAPRAGTQDDKVLSRAEFVIRPYEWIVIGTFTWDGTLV
                     PSTPDWLGDAVVHDVPTTHEKVCRFRSAQCRNVTIWDHAIYETWVSAVTPFDDEFGGG
                     NGECYDYGERE"
     gene            5261..5686
                     /locus_tag="PKH10_03260"
     CDS             5261..5686
                     /locus_tag="PKH10_03260"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HNT27176.1"
                     /translation="MRRLLFLSVLLCAVHLMAQEPGGVTVSVSGLKVTGDLLVTLFDK
                     SDGFPGDYKKARDSRSVKVTAATMSVLFDKLPAGTYAIAVLHDENGNAVVDTGAFGIP
                     KEGIAVSNNITKFFRAPNFDEAKFAHDGKGRTLFIKIVY"
     gene            5824..7026
                     /locus_tag="PKH10_03265"
     CDS             5824..7026
                     /locus_tag="PKH10_03265"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT27177.1"
                     /translation="MKKKIVIAIVVIAAIGGIWYLSSRPAEVAVCRVETKDLNRRLVV
                     MGRVTPQATINVATTFPGRVKEVTAQENETVAKGSLLIALDEKELQANLKVAEAAVSQ
                     SRAKLEQIGGTEARVAEESLNDAKNKLSDAQKNFERDKQLFDKQALSQSEMQRSELAV
                     KTAQSQYDIAATRERSIAKGGNDYNLARAALEQAMANRDLARQRLTDARIYAPFEAMV
                     LSRTVEPGAVAQPGQTLMVVAAAKECYLAADIEERNLGMVHEGQHAIVSSESFPDRRF
                     DATVYQIARTVDPQRGTVRVKLAVAEKPEYLRADMTVSIDLDVEKITSALLVPAVAVS
                     GEGKGTAVLVLNDGRVEKRPVIAAEKENGKVRIVEGVNPGDLVVVPGDKSQPVEGMKA
                     VAKETGGC"
     gene            7026..>8137
                     /locus_tag="PKH10_03270"
     CDS             7026..>8137
                     /locus_tag="PKH10_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT27178.1"
                     /translation="MPFSVFIAFKYLLDEKRQTLFIMSACAIGVAVMVFLYGLISGLQ
                     RSLVDRTLGSQAHIILNANKEKPRPLAESGNDTAVMARVERGFDRPQMISDWPRRIEA
                     VAASSGIVAVAPRLTGAALLRRGDVTRPVGVSGIEPDSFTTIIPLAKSVVAGRYDLVG
                     DEIFVGKDLADDLGVTVGDRVALQTRGGAVNFLISAVFDLGNRSVNENQVFVSLRQAQ
                     ALLGAPGDINQIDTRVGEIFEAEKIARRLEKETGIETISWMEQNSQLLIALRSQSSSS
                     YLIQFFVMVAVALGIASVLVVSVVQKRAEIGILRAFGTKRSVVTRIFLIQGAVVGLLG
                     STVGCFIGTGLAMFFRDSAKNPDGTPRFPISYELSE"
BASE COUNT         1667 a         2274 c         2600 g         1596 t
ORIGIN      
        1 ggcaacggca tcctcgatac cactcctacc cccgagacct gtgacggcgg gatgaaggcc
       61 tgccccgaga tcaacggcat ctacatgttc ggtatggcgc cctgcatcgc tacctgcgac
      121 ggatacgatg agaattactg caagatgagc gccgactgtg agggctaccc cgactatctc
      181 tcgggcgccg atttcaacct gtgctatttc aaaggcaatt atgtgcccga cacggcgcag
      241 ggacagtgct acgacaacag cgcggagatg ggggccgggt gccccgctcc gggagccgat
      301 ttctttgggc aggatgcaca gtacggcggt tcgggaaagt acatctccat ggtggtcacc
      361 gatgtggtgg gggatgacta caccggcctg cagtggcacc agatggtccc gcagttgtac
      421 agcgactgca acagcgagat gtactgcact tgggctcagg cggtgaccta ttgcgagaac
      481 tcgatgtcgg gcggttacag cgactggcgc ctgccgaccg acaaggaact cgagagcatc
      541 atcgagtaca gcggcatcgt tccgatggtg ccggaaggcg ttttcacgat gttcgacgcc
      601 gagtatttct ggagttcgac cgagaatgtg gccaacccga cgatgagcgc gtgggcgacc
      661 tacttcgcga taggtaccag tcagccggcc cccaagacct cgcagtacaa ggcgatctgc
      721 gtgcggggga ccttcttccc gacggatacc aagaccttca cgcatgagtg gcgcggcggc
      781 gacgaggtgt atctcgacga ccagaccggt ctcatgtgga ccggcgacta ctggtacagc
      841 gatgtggtgc cggtgtccga ttggaccgag gcgcttgcgt attgcgagac gggcgagttc
      901 gccggctaca ccgactggcg tcttcccagc atcaacgaac tggggaccac ctacgaccgt
      961 acctgggcga cgccgccggc cgatttcccc ggattctctt cctacgcttt ctggtcctcg
     1021 acctcgcgtc ccgatgcgcc gaccgccgcc tactatctca cgagcggtgg ctcgaccggt
     1081 tcgcaggtga agaccctttc gggcgcggta cggtgcgttc ggtaaaaact aataggttcc
     1141 ggtccgctgt ctgaatccct tgaaaacctg cagatgcgcc gtaaggatcg gccgcttgtc
     1201 gcggaaccgt tcgatgcggg cgtaggcgtt gtgggtgtgg atgctctcga ggtgggtcgc
     1261 ttcggccatg aaccagtgga tatgcggttc ggcgagcagt agttcggccg cgccgcgcac
     1321 gatgtcctcg gcgaaacggg ggcgggtgaa agccttttcg gtgacatatt tctcgtcggg
     1381 ccgcttgagg agcggatata tctcggaact cgcgcagtcc tcgagtctgt ccacgatgtc
     1441 ctcaagccag atgaaatgat cgaacgagag gtaggcggtg atgtaggatc gctgattatg
     1501 cgcgccgaac tcgctgatct cctgcgaaca ggggcagagc gtcgtgaccg gcacgcggat
     1561 accgaggatg aaactggtgc cgttcccgtc gccgtagccg tcgaacagga catcgaccgg
     1621 gaaaagcgac cgcgcctcgg aaacgggcga tttcttctcg atgaagtagg ggaactcgag
     1681 cgacatcatc gccttgggcg atccgagact ctcgcgggtc tccttgagga tgtcgcggat
     1741 gttgcccatc gtgatctcgc cacggtagcg gttgagtatc tcgataaagc ggctcatgtg
     1801 ggtccccttg aattcgggga gcagttcgac gctcatcgat accgccgcga ccgtctcctg
     1861 catgccgcgc agtttgtcct tgaccgttat cgggtagcgg atggccgaga caccgaccat
     1921 gtcgaccggc agtccgcgat cgtcgcgctg gttctgtatg tccttcatcg gatcctccgt
     1981 cggtgcggtg cgcagtataa acggggtggc cgttttggca atataaattt aatgaaagaa
     2041 agcggttgac ttgcttttca agttgcgata gattttaccg tatcaggaag ggagcggtcc
     2101 ttttgaaaca gatgttcatc acttttgcgg tcctgttgtt gtgcgtctcc tgcgggaaca
     2161 cgatcgagat gccgaccgat gtcgaaagcg atgccggatc cgatgtcgga cccgatacgg
     2221 aagcggaaga tgatgcggcg atcgtcgtcg atgaggatgt cgttaaggtg ccgccggctc
     2281 gcgtcatggt gcagttgaca tggaaggagg gattgaaggc ccgcgccgaa ctcgatcgac
     2341 cggaaggggt ggcggtagat ctcgacctgc acctcatcaa gaagaagggg atggacgcct
     2401 gcgggaacga ggtcggcgcc gacggcagga tgtgtacggt gatgatgacg cctgccggaa
     2461 cgaccataga atcctctacc catgatgatt gttattggca tgatgtcggg gaaaacgcag
     2521 tgtattacga ctgtagcgga tgcgaaggag agtgtagaac gatcgcgtgg cacgcggcgt
     2581 tcgttctcga caaccgctgg ggtggcgacg actacgcgga cccggaggtc gtgatcctcg
     2641 gcccgatagc cgacgaggag ccgaaggacg gattccccga tgtgcacccg ttcaacgacg
     2701 agtatctgat agtggtgaac tacaacaact gtcgggacct gacggaaccg tgggacgggc
     2761 cgcaggagag atgtaaagaa ggaggggctt attacaatgt tcacgcgaag gtcgagatat
     2821 tcgtggatga taaggtggcg ccgcaggccg gcacgcagga cgataagtcg ctgagccgga
     2881 ccgagttcgt catccgtccg gacgagtgga tcgtcatcgg tgccttcatc tgggacggcc
     2941 ctctcgtatc ggccgtttcc ggatggccgg gcgacgcgat cgtccgcgat gttcccgtaa
     3001 gtcatgaaaa ggtctgtcgg ttcaactcga accgttgtcg tgatctcccg atatgggatg
     3061 tggccgctta tgaagagtgg gtgaccgcgc cggatgttcc gcaagacttt ttcgccagcg
     3121 gcgacggcga atgctacgat tatggtgaac gggagtaacg gtaagatacg atgaagaact
     3181 tccttgtcac ggtgtcggtc atgtttctct gcctgtatgg atgcaaggag gtaacatctc
     3241 cgcctctgac cgtggcggag ccggccagtt ggcatgtagg accggtggtc agcgaggcgg
     3301 cggccgatgg tcactatacg gcgggggatg gcaccgatct ccctttcatg cggcgtgatt
     3361 ttgttatccg aaacaccggg aaaggagcgg cgcttcgcat ttatagcatc aacttcctgt
     3421 ctccggaggg tacggtgctg tcggatgcgc ttcgttcggc ccttttttcg atagaacgat
     3481 ccacggtcag cgatgcgcac ggtacggtgg tcgcggggtt catgcctgtc gaggagaatc
     3541 attttgagaa ccgttatccc gacgggaccg ctatcgtcgc gccgctgtgt cctcttgact
     3601 cggaggggaa atgcaagaaa ggtttcgatg ccgcgaatca caataccgcg ctgaccatcc
     3661 gggtgactta tcgtggattg acgacggagc agtatgcggc gttggccgtt gacaaactca
     3721 aaagcgatgg gacctattat gtcgaattgt gcgtgaacga ttatgcgaat ccgatcgaaa
     3781 gcgccacctg cacgcagggt aactcgttcc gcattcccat ttatcgttct ccccagacac
     3841 ctcccaaacc gttcatcggc ctggaatatt cgagcccgat cggcgggtac aaggaccacc
     3901 gggtcatcaa ggactgggtc aatatggagg tcaacctctc ctgtccgcga aaagagggcg
     3961 ccgatgagat gacctgcggc aagatcattt ccgatacggt cgtgagcgat gaaacgataa
     4021 cagcggccga gttcaaggaa aaatgttgcc tagccgactg gaaggactgg aaggatcgct
     4081 attgcatcgc ctatcgctgg gagatgatag agacgccgac gcctctcgcg cccgagaccc
     4141 gtatcggttt cggtaacata ccgggcggtg gcgaaggcac atggtatcct gcctgcggag
     4201 aattcgatcc gacgctcgcc tcgttcaagg ggcacatgat caccccgaca cccgagggca
     4261 aatactacaa ggtaagtata caggcgatga cgatagacaa gcagacccag ttggaaagcg
     4321 atatcgtcga aaagatagac actcccaata tcatctcggc ggcccgcgtc atggtgcagt
     4381 tgacatggaa ggagggattg aagacccgcg ccgaactcga tcgaccggaa ggggtggcgg
     4441 tcgatctcga ccttcacctt atcaagaaaa aaggcatgga cgcttgcggg aacgaggtcg
     4501 gcgccgatgg actcatgtgc accgtcatgt cgcgccccgg cctcccgata gacacgccga
     4561 cccatgacga ctgccactgg gacgatctgg ggctgaatgg cgaatatctt ttcaccgact
     4621 gcaacggcga gttcaagacg atcggctggc atgccgtata cgaccttgac aaccgatggg
     4681 gtggcggtaa ctacgcaaat cctgaaataa taaacctcgg gtcggtgatc gatcaaaaga
     4741 ataacagcac cggcgtgcac ggtaaggacg gggttctcga tgtgacccct tttgacgacg
     4801 agtatctggt gatggtgcaa tacaaccact gccaaagcct tcagccgggt tatgatgatg
     4861 ctccgtgcca agacggtggg gcggaataca atgtccatgc gaaggttgag atattcgtgg
     4921 acggcaagtt ggcgccgcgg gccggtacgc aggatgataa agtgctgagc cgggccgagt
     4981 tcgtcatccg tccctacgag tggatcgtca tcgggacctt cacttgggac ggcacccttg
     5041 tcccttcgac accggattgg ctgggggacg cggtcgtcca cgatgtcccg acgacccacg
     5101 agaaagtctg tcggttccgt tcggcccagt gtcgcaatgt taccatatgg gaccatgcta
     5161 tctatgaaac atgggtgagc gccgtgacgc cgttcgatga tgagttcggc ggcggcaacg
     5221 gcgaatgcta cgattatggt gaacgggagt aggaggtctc atgcggcgtt tgttgttcct
     5281 ttccgttctt ctatgcgcgg ttcatctgat ggcgcaggag ccgggcggtg tcacggtgtc
     5341 ggtatcgggg ctcaaggtga cgggcgacct gctcgtgacg ctcttcgaca agtccgacgg
     5401 gttccccggc gattacaaga aggcgcgcga ttcccgcagt gtcaaggtga ccgccgccac
     5461 gatgagcgtc ctgttcgaca aactgcccgc cggaacctac gccatcgcgg tgctccacga
     5521 cgagaacggc aacgcggtgg tcgataccgg agccttcggg ataccgaaag aggggatcgc
     5581 ggtgtcgaac aacatcacca agttcttccg cgcccccaac ttcgacgagg cgaagttcgc
     5641 ccacgacggc aaagggcgca cactgttcat aaagatagtg tactgagacc ttcaccgcta
     5701 tctccttcgc cctgtatagg gagaaggggc ggggatgagg gtgttcatgg tgatattgac
     5761 ccgtttcatt gactcttccc tcgcttctct ctaatatcgc tttgaaccgt aacggagtgt
     5821 cccatgaaaa agaagatagt gatcgcgatc gtcgttatcg ccgccatcgg cggcatctgg
     5881 tatctctcgt cgcgccccgc cgaagtggcg gtctgccgag tcgagaccaa agatctcaac
     5941 cggcggctcg tggtcatggg gcgcgtgacg ccgcaggcga ccatcaatgt cgcgaccacc
     6001 tttccgggcc gcgtcaaaga ggtgacggcg caggagaacg agacggtggc gaagggttcg
     6061 ctcctcatcg cgctggacga gaaggaattg caggcgaacc tcaaagtggc cgaggcggcg
     6121 gtctctcagt cgcgcgccaa actcgaacag atcggcggga ccgaggcgcg cgtcgccgag
     6181 gaatcgctca acgacgcgaa gaacaaactt tccgacgcgc agaagaactt cgagcgcgat
     6241 aaacaactct tcgacaaaca ggcgctctcg cagagtgaaa tgcaacgctc cgaacttgcg
     6301 gtgaagaccg cgcaaagtca gtacgacatc gccgcgaccc gcgaacgctc catcgccaag
     6361 ggaggcaacg actacaatct cgcccgggcg gctctcgaac aggcgatggc caaccgcgat
     6421 ctcgcccgcc agcggctcac cgacgcccgc atctatgctc cgttcgaggc gatggtgctg
     6481 tcgcggaccg ttgaaccggg ggcggtcgcc cagccgggac agacgctcat ggtcgtcgcg
     6541 gcggccaagg agtgttacct cgccgccgat atcgaggagc gcaatctcgg catggtccat
     6601 gaaggacagc acgcgatcgt atcgtccgaa tcgtttcctg accgccgctt cgacgcgacg
     6661 gtctatcaga tagcgcgcac tgtcgatccc cagcgcggca ccgtccgggt gaaattggcg
     6721 gtcgccgaga agcccgagta tctgcgggcc gacatgaccg tctcgataga tctcgatgtg
     6781 gagaagataa cctcggcgct cctcgttccc gccgtcgcgg tgagcggcga ggggaagggg
     6841 acggccgtgc tggtccttaa cgacgggcgc gtcgagaagc gaccggtcat cgccgccgag
     6901 aaggagaacg gcaaggtccg tatcgtggag ggggtgaacc cgggcgatct ggtggtcgtt
     6961 cccggcgaca agtcgcagcc ggtcgaaggg atgaaggcgg tcgccaaaga gacgggaggc
     7021 tgttagtgcc cttttcggta ttcatcgcgt tcaaatatct gctcgacgag aagcggcaga
     7081 cgcttttcat catgtcggcc tgcgccatcg gcgtggcggt gatggtgttc ctctacgggc
     7141 tcatatcggg gcttcagcgg tcgctcgtcg accggacgct cggcagtcag gcgcacatca
     7201 ttctcaacgc gaacaaggaa aagccccggc cgctggccga gtctggcaat gatacggcgg
     7261 tcatggcgcg ggtcgagcgc gggttcgacc ggccgcagat gatctcagac tggccccgcc
     7321 ggatcgaagc ggtcgccgcc tcctccggca tcgtcgcggt cgccccgcgg ctgaccggcg
     7381 cggcgcttct gcggcgcggc gatgtgacgc gcccggtagg ggtgagcggc atcgaacccg
     7441 atagtttcac caccatcata ccgctcgcga aaagcgtcgt cgcggggcgc tacgacctcg
     7501 tgggcgacga gatattcgtc ggcaaggacc ttgccgacga cctcggcgtg acggtgggcg
     7561 accgggtggc gcttcagacg cgcggcggcg cggtcaactt cctcatcagc gcggtgttcg
     7621 acctcggcaa ccgatcggtc aacgagaatc aggtgttcgt ttccctgcgg caggcgcagg
     7681 cgctactggg tgcgccgggc gatatcaacc agatcgatac gcgggtcggc gagatattcg
     7741 aggccgaaaa gatagcgcga aggctcgaga aggagaccgg catcgagacg atatcgtgga
     7801 tggaacagaa ttcgcaactc ctcatcgcgc ttcgctcaca gtcgagttcg agttatctca
     7861 tccagttctt cgtgatggtg gcggtggcgc tcggcatcgc cagcgtgctg gtggtgtcgg
     7921 tcgtgcagaa acgggccgag atcggcatcc tgcgcgcctt cggcaccaaa cggtcggtcg
     7981 tgacgcggat attcctcatt cagggggcgg tcgtgggact cctcggctcg accgtcggct
     8041 gtttcatcgg gaccgggctt gcgatgttct tccgcgattc ggccaagaac cccgacggca
     8101 cgccgcggtt cccgatcagt tacgaacttt cagaata
//
LOCUS       DAOQUE010000068         8087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_9844_length_8087_cov_4.336653, whole genome shotgun sequence.
ACCESSION   DAOQUE010000068 DAOQUE010000000
VERSION     DAOQUE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8087
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9844_length_8087_cov_4.336653"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PKH10_03275"
     CDS             <1..429
                     /locus_tag="PKH10_03275"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNT27179.1"
                     /translation="YSKVRLLNGDTFFVPPASAERVYIVGEIARPGAYQITKPDFTVI
                     DLIAEAGGINPITAETSEIYLVRQYGGKFLYAQMNYYKILGGDIESDLRLQPGDRLIV
                     SPTGLASYNRIIQQLLPTFQLLESATGAVQNTISIENTLR"
     gene            complement(475..1848)
                     /locus_tag="PKH10_03280"
     CDS             complement(475..1848)
                     /locus_tag="PKH10_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27180.1"
                     /translation="MKNNRSFSTDADGAILRFKYYTLLFFLFMSPFAFSSFVNRVSPV
                     LPTATLLLLATTYVFGLFLLRTKKLFLVPSERVVLIGLFLPFLFSIPLVGANFLFHAD
                     SAYALYVSMKLPGRLANAVFFFSLFVLAMSFIGEYRSRPPESFARWYFAGTAVLVFFG
                     IWQWLHNYLNVPMFDLDTRSYMFGLRQNGFSYLVDRIRLTSLLDEPNFLVAFLIDGLL
                     LGPLLVTSRRYYAFGVVAPFLFVLLFSFSAGGYLNLLLLCGVVLLTPGFLPAKIKKKA
                     VAITIAALFLAGTIFYQQIFEFLFPILGRLDSLFNQTSTRIYMMVSTLRAAWDDPLPF
                     MIFGHGPGSVFYLNFKGLPMATSNSMYLDLFYETGLFGLLAALWMFSRLLKENISFGW
                     TRDPRVRWIYLLICHLAITSTYRADFFSSRFWSILILVLAFHHSFREGTSSSIATSEE
                     RRSPKPL"
     gene            complement(1845..2222)
                     /locus_tag="PKH10_03285"
     CDS             complement(1845..2222)
                     /locus_tag="PKH10_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27181.1"
                     /translation="MTFDAEGTPSAKGLMPGDSLKEGSVNEKLLHTHVFSGGVPPLLF
                     IDTPFFRDHMRSITGGDTIFLVLGNPPSEGKIPLPPDMIFSAVFATVRFGVTRQEDIR
                     RISDLVSESSGETKGKNGYLFLV"
     gene            complement(2305..3384)
                     /locus_tag="PKH10_03290"
     CDS             complement(2305..3384)
                     /locus_tag="PKH10_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27182.1"
                     /translation="MNKLLEYLAELLEVAPLDNAKDSSYISLSVESEYPYLATEIVKE
                     VLNAYMEKKKTWAVDNANQQQKLIEKVLQDVKVELDALSEQMARYQSEKKTVLPEIQL
                     SAIMEKLVDLDKETEGLRLKSKAIRESLQTLDRSPDEPVSVPLLLQDLSVQEAARALN
                     SLIMEEARQKQLKTDLHPDLQKLRQSVKDAHANLRTLLLKSLDTYENGTKILEAASKE
                     IGKGIAELPVNIMDVSSFRRRIAITEKLYAFLSEKLYETTITMNSEQRPIDTRCGDPG
                     CAEILSPGPRYIDIDLLLRPLHGAKCLGGHGDPPTDGGYGTGPVEISRVLILHAESDR
                     ARHCSCSHAPCAARALFDPAEIPRL"
     gene            4200..5075
                     /gene="rfbA"
                     /locus_tag="PKH10_03295"
     CDS             4200..5075
                     /gene="rfbA"
                     /locus_tag="PKH10_03295"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNT27183.1"
                     /translation="MKGIILAGGSGTRLYPITRTVCKQLLPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPEDLPRFRDLFGDGAQLGLSLSYAVQTAPRGLADAFIVGEKFIGRDRV
                     SLILGDNIFIGHGLRDYLDRAVARPDGATVYGYRVTDPERYGVVSFDAAGKAETLEEK
                     PQNPRSHYAVVGLYFYDNTVVEVAKQVKPSHRGEIEITDVNRHYLAQGKLHVEIFQRG
                     FAWLDTGTFDSLADAAMFVKAIEERQGLKIGCVEEVAYRMGFIDAGQLEKLAEPVRKS
                     GYGEYLLDILRHKRP"
     gene            5072..6139
                     /gene="rfbB"
                     /locus_tag="PKH10_03300"
     CDS             5072..6139
                     /gene="rfbB"
                     /locus_tag="PKH10_03300"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNT27184.1"
                     /translation="MRTPKNILVTGGCGFIGSNFIRYLLARKDFTGRIVNLDALTYAG
                     NPQSLADLAETHPDRYRFVHGDICDEAVVKRTFDEHAVDTVVHFAAESHVDRSISGPA
                     AFIRTNLVGTFTLLEAARAAWKTRTDVLFHHISTDEVYGSLGPTGRFLETTAYDPRSP
                     YSASKAGSDHLVSAYHHTYGLPVTISNCSNNFGPYQFPEKLIPLMILNLGEGKPLPVY
                     GDGGNVRDWLHVADHCAAIGEIIARGRAGETYNIGGNNEWKNIDIVHLLCDILAEQTG
                     KRKEEYTKLITFVADRPGHDRRYAIDCGKIERELGWRPTPDFRALLTATVRWYLDNPA
                     WIASVRTGEYRRWLDTHYGAR"
     gene            6144..7424
                     /locus_tag="PKH10_03305"
     CDS             6144..7424
                     /locus_tag="PKH10_03305"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: protein motif:HMM:NF019073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0036065 - fucosylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HNT27185.1"
                     /translation="MTGDRPVAIRFLHLVQDSPLMDGILARFDEMVAHNRYLFRTEKA
                     RYTFRNITRRDRVEVFAAADRELARVLADGDTYDVLVLHSLYAVDPDLLSVLPDRVRV
                     WWISWGYDLYGPHLIDTELFGERTAALRRELGRNGMGGFFKKGDLRDLGETLFFHPRP
                     FSLIHERLRKRLARRRETDAERGTRERTALHRAIGRITYCSTTLPEEYELLAPKAFFT
                     PRYIPFNYVTHDLFDAGTVESAPFCDGDGILIGNSATFENNHADVLEHLRRAPLPGGR
                     IVVPLSYGDPVYGDRITQLGRELFGDRFHPVLDFLPFPQYAALLRSCPTALFGHERQQ
                     ALGAVLIALWNGSTVFLSPTSVIYRHLKNLGVDVLSLSDDLPRRTAPTLLPRDTLLAR
                     RRIISREYSSATRVARMHAAIERIAREIPSTLAQ"
     gene            7511..>8087
                     /locus_tag="PKH10_03310"
     CDS             7511..>8087
                     /locus_tag="PKH10_03310"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNT27186.1"
                     /translation="MADTSLKEKTVSGVAWAAVDQFSNQFVLFMVSVFIARILSPEDY
                     GVIAIVMIFVSLSAILVNSGFGTAVIQRREISPELCSSVFFFNVTVSVLLYIALYLAS
                     PLIASFFDMPVLASVLPLLGIVIIINATYLMQVSLLTRELDFKKQSFVHLTATLVSGS
                     IGLSMAYAGYGVWSLVAQSLSNSLTTSIAYWI"
BASE COUNT         1847 a         2442 c         2143 g         1655 t
ORIGIN      
        1 tattcgaaag tccgactctt gaacggcgac acctttttcg tcccccccgc atcggccgag
       61 cgggtatata tcgtcggcga aatagcgcgc cccggcgcct atcagataac caagcccgac
      121 tttaccgtca tcgatctcat cgccgaagcg ggcggcatca accccatcac tgccgaaaca
      181 tcggagatct atctggtccg acagtacggc ggcaaattcc tctatgcgca gatgaactac
      241 tacaagatac tgggcggcga catcgagagc gacctgcgac ttcaaccggg cgatcgcctc
      301 atcgtatcgc ccaccggcct agccagttac aaccgcatca tccagcaact tctgccgacc
      361 ttccaactgc ttgagtcggc cacgggggcc gtccagaaca caataagcat agaaaacacc
      421 cttcgttaac gcccccactt cccagccatc catcagtcta gttattgcgt cgctctacaa
      481 tggctttggg gatcgtctct cttccgaagt agcgatcgaa gaagatgttc cctctctgaa
      541 actgtgatga aaggccagca cgagtatcaa aatgctccag aatctcgatg agaagaaatc
      601 ggcgcgatag gtcgatgtta tcgccagatg acagataagg aggtatatcc aacggacgcg
      661 gggatcccgg gtccaaccga aagaaatgtt ctccttcaac aagcgagaaa acatccacag
      721 agcggcaagc aggccaaaca ggccggtctc gtaaaaaagg tcgagataca tgctgttgga
      781 tgtcgccatc ggcaaccctt tgaaattaag atagaatacc gatcccggcc catgcccgaa
      841 gatcataaag ggaagtggat catcccacgc cgctcgcagg gtcgagacca tcatgtatat
      901 ccttgtgctg gtctggttaa aaagagaatc aagacgaccg aggatcggaa aaaggaattc
      961 gaatatctgt tggtagaaga tggttcctgc caggaaaagc gccgcgatgg ttatcgcaac
     1021 agcctttttt ttgatcttag ccggcaaaaa gcccggcgta aggagaacaa caccgcataa
     1081 aagtaagagg ttaagatacc cccccgccga aaaagaaaaa aggagcacga aaaggaacgg
     1141 tgccactacg ccgaacgcat aataacgacg agaggtcacc aggagaggtc cgagcaaaag
     1201 cccgtcgata aggaacgcca cgaggaaatt aggttcatca aggagagaag tgaggcgtat
     1261 ccggtcgacc agataagaaa acccgttctg gcgcaggccg aacatataag atctcgtgtc
     1321 cagatcgaac atgggcacat tcaaatagtt gtgaagccat tgccatatac caaagaaaac
     1381 caacaccgct gttcccgcga aataccaacg ggcaaaggat tccggcggtc gggagcgata
     1441 ctctcctatg aaagacatcg ccaagacaaa aagggagaaa aagaaaacgg cattggcgag
     1501 ccttccgggg agtttcatcg aaacatagag cgcataggca gaatcagcat ggaacaggaa
     1561 attggccccc accagaggta tcgagaaaag gaacggcagg aaaaggccta tcaggacgac
     1621 ccgttccgac ggcacaagaa ataacttttt tgttctcaac aggaagagac cgaacacata
     1681 cgtggtggca agcaggagta gcgtggcggt cggaagaacg ggagaaaccc tgttaacgaa
     1741 cgaagagaaa gcgaacgggg acatgaacag gaaaaaaagc agggtgtaat acttgaaacg
     1801 taggatggcc ccgtcagcgt cggtagaaaa ggacctgttg ttcttcatac aaggaaaaga
     1861 tacccgttct tacctttcgt ttcaccggaa ctttctgaga caagatcgct gatcctccgg
     1921 atatcctcct gccgggtgac accgaaacga acggtcgcga agaccgcgga aaatatcata
     1981 tccggtggaa gggggatctt tccctcggac ggaggattgc ccaacaccag gaaaatcgtg
     2041 tcgccaccgg tgatcgatct catatggtcc ctgaagaacg gcgtatcgat gaagagaagc
     2101 ggcgggacgc caccggaaaa gacatgggta tgcaggagtt tttcgttaac tgatccctcc
     2161 ttcagagagt ctccgggcat cagccccttc gccgaaggag ttccctccgc atcgaaggtc
     2221 atgagacgaa ctctacctcc ccctgacaat gacgcaaggg tgtctttgat atcccctatc
     2281 aattgttccc cccccatcgc caggtcaaag acgaggaatt tctgcggggt cgaagagggc
     2341 tcgcgccgca catggagcat ggctacacga acaatgtctc gcgcggtcgc tttctgcgtg
     2401 gagaatgaga acacgggata tttcaacagg tcctgttcca tatccaccgt cggtcggagg
     2461 atctccatga ccaccaagac atttagcacc atgaagagga cgaagaagaa gatcaatatc
     2521 aatgtatcgc gggcccgggg aaaggatttc agcacatccg gggtcgccac atcgagtatc
     2581 tatcggtcgc tgttccgaat tcatcgtgat ggtcgtttcg tagagtttct cggaaaggaa
     2641 ggcgtagagt ttttcagtga tggctatcct gcgacggaat gaggagacat ccattatgtt
     2701 caccggcagt tccgcgatac ccttgccgat ctctttcgat gcggcctcca gtatcttggt
     2761 cccattctca taggtatcga gacttttcag gaggagcgtg cgtaggttcg catgagcatc
     2821 cttcaccgat tggcgaagtt tctggagatc cggatgaaga tcggtcttga gttgcttttg
     2881 acgggcctcc tccatgatga gcgagttaag ggcccgggcc gcctcttgaa cggaaagatc
     2941 ctgcaacaac aacggcacgg aaaccggctc atcgggagac cgatcaagcg tctgcaacga
     3001 ttctcgaatg gcttttgact ttagtctcag accttcggtt tccttgtcga gatcgaccag
     3061 tttctccatt atggcggaaa gttgtatctc aggaaggacc gtttttttct cggattgata
     3121 tcgagccatc tgttcactaa gggcatcaag ctcgaccttg acatcctgca ggaccttttc
     3181 tatgagtttc tgttgttgat tggcgttatc caccgcccac gtcttttttt tctccatata
     3241 agcattgagg acctccttca ctatctccgt cgccagataa ggatactccg attcgacgga
     3301 tagcgagata taggaactgt cctttgcgtt gtccagcggc gccacctcca aaagttcggc
     3361 aaggtactcg agaagtttat tcatctcgaa ataagtcact gtaaaatgtg cgttttcagg
     3421 gatatcccct aactgtcgca cataaagggt cccgccgggc agggaacaat ccttttggaa
     3481 ttcgcagtcg aattcctgcc cgtttggatg cgtgacccgg taacgcccct ctcttcccac
     3541 ggttatcttt atatcccggt cgcgaaaatc ctcggggcat cgttcgagga tcatggatcc
     3601 atatgggaga cgaatccccc ggaaacgatc gagaaaataa aaaagcatat tattggacgt
     3661 tcgttgcggg aagaactgta gattgagggc cttgacggca ttttcaatga tcgccctgct
     3721 tcgtagtatg accgcttcgt tctctgtcga gttgatattg ttgttcataa gcatcatgat
     3781 gggagaaagc gtcatctgtt cgtccttttg ttctcccgcg taaaaagtgg aaacaatggt
     3841 gtacctcggc gtcactagga agaacgccac caaagaaaag agaagagaaa gggccgccgt
     3901 gatcgcaagt ggcttcgcat gaagggataa cagatcctta agtcgcgtga aggtcaccga
     3961 ctcctctatt tcgtgactca cgaccagaaa tcctcccgga acaaaaaaga gagcgctttc
     4021 ttaaaagttc aagcatcgtc gttgtagcat gtttcagcag aagcgtcaat ttttaacact
     4081 tttctatttg catcaacctc tttctcggga tctgccatgt catcatagat tgcttttttc
     4141 ttgcttctcg acttgcgctc cctattatcg tagagtactt ttttcgacgg agagagacca
     4201 tgaaaggcat cattctggcg ggcggcagcg gaacgcggct ctatccgatc acccggacgg
     4261 tctgcaagca actgctcccg gtctacgaca aaccgatgat ctattacccg ctctcgaccc
     4321 tcatgctggc ggggatccgc gatatcctga tcatctcgac cccggaggat ctgccgcgct
     4381 tccgggacct gttcggcgac ggcgcgcaac tgggactgtc gctctcctac gcggtgcaga
     4441 ccgcgccgcg cgggcttgcc gacgccttca tcgtggggga gaaattcatc ggtcgcgacc
     4501 gcgtttcgct gatactgggc gacaacatct tcatcggcca cggcctgcgc gactatctcg
     4561 accgggccgt cgcgcggccc gacggggcga ccgtctacgg ctaccgggtg accgatccgg
     4621 agcgctacgg cgtggtgtcg ttcgacgccg ccggcaaggc cgaaacgctg gaggagaagc
     4681 cgcagaaccc ccgttcccac tacgcggtgg tggggctcta cttctacgac aacacggtgg
     4741 tcgaagtggc gaagcaggtc aagccgtcgc accgcggcga gatcgagatc accgatgtca
     4801 accgccatta cctcgcgcag gggaaactcc atgtcgagat attccagcgt gggttcgcat
     4861 ggctcgatac cggcaccttc gacagtctcg ccgacgccgc gatgttcgtc aaggcgatcg
     4921 aggagcggca ggggctcaag atcggctgcg tcgaggaggt cgcctaccgc atgggcttca
     4981 tcgacgccgg gcaactggaa aaactcgccg aaccggtccg caagtcgggc tacggcgaat
     5041 atctgctcga tatcctgagg cataaacgcc catgagaaca ccgaagaaca tcctcgtcac
     5101 cggcgggtgc ggcttcatcg gaagcaattt catccgttac ctgctggcgc gcaaagactt
     5161 cacgggacgc atcgtcaacc tcgacgcgct gacctacgcc ggcaatccgc agagcctcgc
     5221 cgacctcgcc gaaacgcatc ccgaccgcta ccgttttgtc cacggcgaca tctgcgacga
     5281 ggcggtcgtg aaacggacgt tcgacgagca cgccgtcgac accgtcgtcc acttcgccgc
     5341 cgaatcgcat gtcgatcgct ccatcagcgg cccggccgcc ttcatccgga ccaatctcgt
     5401 cggcaccttc acgctcctcg aagcggcccg cgcggcatgg aagacccgca ccgatgtgct
     5461 gttccaccac atatcgaccg acgaggtcta cggcagtctc ggcccgaccg gccgctttct
     5521 cgagacgacc gcctacgacc cgcgcagtcc ctattcggcc tcgaaggcgg ggtccgacca
     5581 tctggtcagc gcctaccatc acacctacgg cctgccggtc accatctcca actgttcgaa
     5641 caacttcggc ccctaccagt tccccgaaaa actgataccg ctcatgatcc tcaacctcgg
     5701 cgaggggaaa ccgctcccgg tctacggcga cggcggcaat gtccgcgact ggctccatgt
     5761 cgccgaccac tgcgcggcga tcggagagat catcgcccgg ggacgcgccg gcgagaccta
     5821 caacatcggc gggaacaacg agtggaagaa tatcgacatc gtccatctcc tgtgcgacat
     5881 cctggcggag cagaccggga aacggaagga ggagtacacg aaactcatca ccttcgttgc
     5941 cgaccggccg ggccacgacc gccgttacgc gatagactgc gggaagatcg aacgcgaact
     6001 cggctggcgg cctacccccg acttccgcgc gctcctcacc gccacggtcc gctggtatct
     6061 cgacaacccg gcgtggatcg catcggtccg caccggcgaa taccgccgct ggctggacac
     6121 acactacggc gcacggtgag acgatgaccg gcgaccggcc cgtcgctatc cgtttcctcc
     6181 acctcgtgca ggacagcccc ctcatggacg gcatactggc tcggttcgac gagatggtcg
     6241 cgcacaaccg ctatctcttc cgcaccgaaa aagcgcgcta taccttccgg aacatcacgc
     6301 gccgcgaccg ggtcgaggtg ttcgccgccg ccgaccgcga actggcacgc gtactggccg
     6361 atggcgacac ctacgacgtg ctggtgctcc attcgctcta cgccgtggac cccgatctgc
     6421 tctccgtgtt gcccgaccgg gtgcgggtct ggtggatatc gtggggctac gatctctacg
     6481 gaccgcacct catcgacacc gaactgttcg gcgaacggac cgccgccctg cggcgcgaac
     6541 tcggccggaa cgggatgggg ggctttttca agaaagggga cctgcgcgat ctgggtgaga
     6601 cgctcttttt ccatccccgc cccttctcgc tgatccacga acggctgcgg aaacggctcg
     6661 cccgccggcg cgagaccgac gcggaacggg gaacgcgcga acggaccgcg ctccaccggg
     6721 cgatcggccg gataacctac tgctccacca cactccccga ggaatacgaa ctcctcgcgc
     6781 cgaaagcgtt cttcaccccc cgctatatcc ccttcaacta tgtcacccac gacctcttcg
     6841 acgcggggac cgtggaaagc gcgcccttct gcgacggcga cggcatcctg atcggcaact
     6901 cggccacctt cgagaacaac cacgccgacg ttctcgaaca cctccgccgc gcgccgctgc
     6961 ccggcgggcg catcgtcgtg ccgctcagtt acggcgatcc cgtctacggc gaccggatca
     7021 cgcaactcgg ccgggaactg ttcggcgacc ggttccaccc ggtgctggac ttccttccct
     7081 tcccgcagta cgcggcgctc ctgcgctcct gccccactgc cctgttcggc cacgaacggc
     7141 agcaggcgct cggtgcggtg ctcatcgcgc tgtggaacgg ctccaccgtt ttcctctcgc
     7201 ccacctcggt catctaccgc cacctgaaga acctcggcgt ggatgttctc tcgctctccg
     7261 acgacctgcc gcgccggacc gcgccaaccc tgcttccccg cgacaccctc cttgcccggc
     7321 gccgcataat atcccgcgaa tactcctccg cgacgcgcgt tgcccggatg cacgccgcca
     7381 tcgaacggat cgcacgggag atcccctcaa cactggccca atagggaacc gccaagaggc
     7441 gccttactgt tttcttgctt tacccatctt cccactgtta taatcgcgcg gcaacttgat
     7501 cgaggggtcc atggcggaca cttccctgaa agaaaaaacc gtatcaggcg tcgcatgggc
     7561 ggccgttgat caattctcta atcaattcgt cctcttcatg gtctccgtgt tcatcgcgcg
     7621 gatactttcg cccgaggact acggagtgat cgcgatcgtc atgatattcg tttccctttc
     7681 ggctatcttg gtcaacagcg gcttcggcac cgcggtgatc cagagacggg agatatcgcc
     7741 agaactttgc tcatccgtct tcttcttcaa tgtgaccgtt tcggtccttc tctatatcgc
     7801 actttacctt gcctcccccc tcatcgcctc atttttcgat atgccggtgc ttgcctcagt
     7861 cctccctctg ttgggtatcg taataatcat caacgcgacc tacctgatgc aggtctcact
     7921 tctcacgagg gaactcgact tcaaaaaaca atccttcgta cacctgacgg cgaccttggt
     7981 gtcgggctct atcggtcttt ctatggcata tgccggctac ggcgtctggt cactcgtcgc
     8041 gcaatcgcta tcgaattctc ttaccacatc gatcgcgtac tggatag
//
LOCUS       DAOQUE010000069         7898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_10138_length_7898_cov_3.915339, whole genome shotgun sequence.
ACCESSION   DAOQUE010000069 DAOQUE010000000
VERSION     DAOQUE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10138_length_7898_cov_3.915339"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PKH10_03315"
     CDS             <1..531
                     /locus_tag="PKH10_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27187.1"
                     /translation="LRGAILANIKEVASLRALYEPLLAEEPMNNIPYLIPYGLNLLFP
                     ALAPYEAMLQTALRTVLLGAVAIVTVWQIIRLARRRDEPFLVFLRIMVLVICFTSLRF
                     HSWYFIAVLPLFAAGTPRVWLRWGVWVFPLTLLFDIHNFVPRDSWVLLALLPPAVVLV
                     NLLFLFKLPERFAGRT"
     gene            528..2567
                     /locus_tag="PKH10_03320"
     CDS             528..2567
                     /locus_tag="PKH10_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27188.1"
                     /translation="MRRAIVVLAVSVTVLFFALLLAHGVVMPILFPEECSADRTIDAF
                     AAAPPPGALPDPAERADHEAAAALYRRKAAEAIGLHFLPATEPAVRAVGEDIVAERYR
                     LRRMIVTAAGPLEAPANLYLPADAPAPFPALVFVAGDGRGKGYAPYRRLAATLAASGI
                     AVFVLDLPGVGERAEGEEGIAALLSLGITVPGIAAREVLAAADWLRGLPGVDGGRIMA
                     AGHAEGGTAALYALAIEPRLSAGILLLSLDDNGAMIRERRISELSRVTIFPRRDIEQH
                     QLVGLVAPRPLLVVAGKQDRRRAAAATETVRKGRVTYRFLGAEEELDLALVDGSGPLL
                     PSHRAALYEFLRDHGLPAGRSEDETEPPSEGDTVVGIEAVPAGALAAFAARETAATRA
                     AIRAMRDGEGPLAYATRLAEFLRDLSGEGLFGPATHEAVVREERTVEKMKEEELSFYP
                     APDTVLSARLTGPLPPARAVIVVTGTRAAGRTARALWSEGLVVLEMELRRGSSDRLWG
                     IASTADPERRTAAAALVAGVPLAFLRAQDILSAAAYLRSRADRVTAGPIGLYADGPEA
                     TLAALLAADLSGELAPVVLTDLPITMAPGAAPVPGMALHAAPLLRLADIGDLISACAP
                     RRMMIGGARGPGDAAVDTVAIREQVWETRHLVVDTVPDRDRIVRFFVTGGTTPAP"
     gene            2656..4251
                     /locus_tag="PKH10_03325"
     CDS             2656..4251
                     /locus_tag="PKH10_03325"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNT27189.1"
                     /translation="MRRLVWLALSVLALAVVSCEEKKVSACLEECPAGWACNESTGLC
                     EEVSGDVDTVTDTDTDKDADTDKVPPDGNDTDDGGEVDDTLTDDDDTFIPDIDMDIDT
                     DGMACVPMTQETCPYTGPENTEGVGTCKAGKRICKEDGSGWTACSGQVTPLYDTCGDE
                     IDQDCNGTADDLDQDKDGVTECGGVDCCYTTEGEEGCPDPKNVYPGAPEIPNGLDDNC
                     NDEIDEGLYDCDGSVLDTSTDPMDFAKVIGLCATTTEQDTGWGVISAEFLLPIGTGTP
                     HAQSHGIFPSFGTVLTPKAGDRMAMLTTGFAANPFDSDKFGGKQMVAQSSAPADWLTL
                     NGNVFPAAPVCGGAGTAGSVNDPVMFKLRVRVPDAAKSFSVNVYFFSQEYVKYVCSDY
                     NDFFLTLLDSTYVAPVENPELANPTDKNLAMDGDGNPVGVNLAPAGLFKQCVNGTSDA
                     GTVITTCVDTNELAGTGFSSYGGTGWLTTRGNVKGGEVIELRFVIWDTGPSGSPDYIY
                     NSAVLIDNFQWQYEEYEPGTIES"
     gene            4255..6621
                     /locus_tag="PKH10_03330"
     CDS             4255..6621
                     /locus_tag="PKH10_03330"
                     /inference="COORDINATES: protein motif:HMM:NF018504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase PrkA"
                     /protein_id="HNT27190.1"
                     /translation="MEDTQQKKSEKRTRKRLKDIYAHHIVEVPGEEPRERLGALISLG
                     TRLRDRDRREPLSFDRFLRLAADRPELMFRDIFQLFHDMVHRYVRSGRDERSSDPESV
                     GFAGYDCDDLFVRGHDNPFFADRLFANRLMTLVNGFKKGLRDNHIILFEGPPGSGKST
                     FLNTILQRLEEYVRTAEGSYFKTYWRLNIERLGGYQNIERPLRRAADGAESPEMHEHL
                     DQMQDLDTRLPKKFLTFSCPSGDHPILQIPRSYRREFLDELIAKGSFKDKLFHRKEYE
                     WVFKGIPCAICRSLYDILLDILGDPLEVFNMICARQMHFNRQFGSGISIFNPGDPVYR
                     KPITNPRLQRLISDLLKNDGVEFIYSVLAKTNNGVYALMDIKENNIERLRDLHGIISD
                     GVHKVDLTEERVKSLFVGLVNPEDKVHYQDVKSFQDRIITVRIPYILDYNTEVAVYRN
                     KFGKAIDRLFLPGVLTNVAKIVVASRLEKDSPTIRAWIGDTDRYRKYMDADALLLKMD
                     LYTGHIPDWLSEEDVRKFDRAVRTDLLAESEQEGFKGFSGRQSLNIFAQLLTNYCEPG
                     TPITMEMVTRFFRQPGEPLFDEIPGDFLDSIEDLYDYGVLQEVRTALSFFNEDQISRD
                     IQNYLFAINFDIGAVEKNAATGDTVAVTEEYLHGLEGILLGADASERRRAEFREETHA
                     EYVTVTVTQEMKVEGKELRDTALYRTLFDRYTRNLRENALAPYAANENFRRAVTDLGT
                     AAFSVYDERIQRDVRELIRILRTKFRYTQEGARQIALYVIEKKLHEKY"
     gene            complement(6640..7431)
                     /locus_tag="PKH10_03335"
     CDS             complement(6640..7431)
                     /locus_tag="PKH10_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27191.1"
                     /translation="MKKHLFNGMLAAIVLIVAILIGWRYHAYLNYRETVKEELARPDT
                     ITEKFSKELASSPAPEVAPPAPTDLTAPPAAGLRETTDGAFQVKKSYTEHGRQVVVKS
                     AGDAKAYKKAGSKFAQVEPNWESPYNLSYQGLRKVNLYLRLHDKWPQEVIQQLNGAAV
                     TISGIVMPTGPVSNDGTMLEFWLANPIVVMAGCVFCNPPTFADLLHVETMSGLKPLKV
                     DREQLYTDTIPVTVTGRLTFGYRKTGDGVESLFQLEMRDWNIIER"
     gene            complement(7436..>7898)
                     /locus_tag="PKH10_03340"
     CDS             complement(7436..>7898)
                     /locus_tag="PKH10_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT27192.1"
                     /translation="TGTLLTETMLVGAVNDDQAHLAATARFIAALKPKKAYILVPTRP
                     PARLDIVLPTPDALAAAWQIFNAALPPDTAEMLTGHEGNAFSSTGDARADILAITAVH
                     PLRTANIESILAAAGASWDIVESLKNEGLVSEVTFGADRFIVRTLHTFTKG"
BASE COUNT         1393 a         2504 c         2579 g         1422 t
ORIGIN      
        1 ctgcgcgggg cgatcctggc caatatcaag gaggtcgcca gcctgcgggc cctgtacgag
       61 ccgcttctcg ccgaggagcc gatgaacaac ataccttacc tcatccccta cggactgaac
      121 ctgctgttcc ccgcgttggc cccgtacgag gcgatgctcc agaccgccct gcggaccgtg
      181 ctgctcggcg ccgtcgcgat cgtcacggtg tggcagatca tccgtctcgc gcggcgccgg
      241 gacgagccct tcctcgtctt cctgcggatc atggtgctcg tcatctgctt cacctcgctc
      301 cggttccaca gttggtattt catcgcggtc ctgccgctgt tcgcggcggg aacgccgcgg
      361 gtgtggctcc ggtggggcgt gtgggtgttc ccgctcacgc tcctgttcga tatccataac
      421 ttcgtgccgc gggattcgtg ggtgcttctg gcgctcctgc cgccggcggt ggtgctcgtc
      481 aatctcctgt tcctgttcaa actgccggag cggttcgccg ggcggacatg agacgcgcga
      541 tcgtcgttct tgcggtatcg gtaacggtcc tgttcttcgc cctcctgctg gcgcatggcg
      601 tcgtgatgcc gatcctcttc ccggaggagt gttccgccga ccgcacgata gacgcgttcg
      661 cggcggcacc gccgcccggc gctctgcctg atcccgccga acgcgccgac cacgaggcgg
      721 ccgccgcgct gtaccgtcgg aaggcggccg aggcgatcgg gcttcatttt cttcccgcga
      781 ccgagcccgc cgtccgtgct gtcggcgagg atatcgtcgc cgaacgctac cggctccggc
      841 gcatgatcgt gaccgccgcc ggaccgcttg aggcgccggc gaacctctat ctgcccgccg
      901 atgcgccggc gcccttcccg gctctcgtgt tcgtcgccgg ggacgggcgc ggcaagggct
      961 acgcccccta ccgtcggctt gccgctacgc tcgccgcgag cgggatcgcc gtgttcgtgc
     1021 tggacctgcc gggggtcggc gaacgggccg agggggagga gggtatcgcc gcgctgcttt
     1081 cgctcggtat caccgttccc ggtatcgcgg cgcgcgaggt gctcgcggcc gccgattggt
     1141 tgcgcgggct ccccggggtg gatggcggtc ggatcatggc ggccggtcac gcggaaggcg
     1201 ggaccgccgc gctctacgcg cttgcgatcg aaccgcgtct gtcggccggc atcctgttgc
     1261 tgtcactcga cgataacggt gcgatgatac gcgaacggcg tatttccgag ctttcccggg
     1321 tgacgatctt tccccgtcgc gatatcgaac agcaccagct ggtcgggctt gtcgcgccgc
     1381 gtccgttgct cgtcgtcgcc ggaaaacagg atcggcgccg cgccgcggcg gcgaccgaaa
     1441 cggtgcgcaa ggggcgcgtg acctaccggt tcctcggggc cgaggaggaa ctcgaccttg
     1501 ccctcgttga cgggagcggt ccgctgttgc cgtcgcaccg cgcggcgctg tacgaattcc
     1561 tgcgcgacca cggtctgccc gccggtcgtt ccgaggatga gacggagccg ccgtccgaag
     1621 gcgacacggt cgtcggtatc gaagcggtcc ccgccggtgc gcttgccgcc ttcgccgccc
     1681 gcgagacggc ggcgacccgt gcggcgatcc gcgcgatgcg tgacggggag gggccgctcg
     1741 cctacgctac ccggcttgcc gaattcctgc gtgacctgtc gggagagggg ctgttcggcc
     1801 cggcgaccca cgaggcggtg gtgcgggagg aacggaccgt ggaaaagatg aaagaggagg
     1861 aactctcatt ctatccagcg cccgatacgg tcctttcggc ccgtctgacc gggccgctcc
     1921 ccccggcgcg ggcggtcatc gtcgtgaccg ggacgcgggc ggcgggacga accgcccgtg
     1981 ccctgtggtc cgaagggctg gtggtgctgg agatggaact gcggcgcggg agttcggacc
     2041 gcttgtgggg gatagcgtcg accgccgacc ccgaacggcg tacggcggcg gcggcgcttg
     2101 tggccggggt gccgctcgct tttctgcggg cgcaggacat cctgtcggcg gcggcctacc
     2161 tgcgaagccg ggccgatcgg gtgaccgccg gcccgatcgg gctgtacgcc gacgggcccg
     2221 aagcgacgct cgcggcgctc ctcgcggctg acctgtcggg cgaactggcg cccgtggtgc
     2281 tgaccgacct tcccatcacg atggcgcccg gcgccgcgcc cgttcccggc atggcgcttc
     2341 atgcggcgcc gctcctgcgg ctcgccgata tcggcgatct catctcggcc tgcgcgccac
     2401 gccgcatgat gatcggcggc gcccgcgggc ccggcgacgc ggcggtcgat acggtcgcga
     2461 tacgcgagca ggtctgggaa acccgccatc tagtggtcga tacggtgccc gaccgggacc
     2521 gcatcgtccg cttcttcgtc accggcggga cgacgccggc gccctgaaaa agagtgcaag
     2581 gttctgtttg cgttttgaaa agagatatgc taccataccc cgtgttttac ggataactgt
     2641 cagtggggaa ccgcgatgag aagacttgtg tggctggccc tgtccgttct ggcgcttgcc
     2701 gttgtctcct gcgaggagaa gaaggtttcg gcgtgtcttg aggaatgtcc cgccgggtgg
     2761 gcgtgcaacg agagcaccgg actctgcgag gaggtgagcg gcgatgtcga caccgttacc
     2821 gacaccgata ccgacaagga cgccgacacc gacaaggtgc ctcccgacgg taacgatacc
     2881 gatgatggcg gcgaggtgga cgatacgctg accgatgacg acgacacctt catccccgat
     2941 atcgacatgg acatcgacac cgacgggatg gcgtgcgtcc cgatgacgca ggagacctgt
     3001 ccctacaccg gcccggagaa taccgaaggg gtcggcacct gcaaggcggg taaaaggata
     3061 tgcaaggagg acggatcggg gtggaccgcc tgttcgggac aggtgacccc gctctatgac
     3121 acctgcggcg acgagataga tcaggactgc aacgggaccg ccgacgatct cgatcaggac
     3181 aaggatggcg tcaccgaatg cggcggggtc gactgctgtt acaccaccga gggcgaggag
     3241 ggctgtcccg acccgaaaaa tgtctacccc ggcgctcccg agatacccaa cgggctggat
     3301 gacaactgca acgacgagat agacgaaggt ctgtacgact gcgacgggag cgttctcgac
     3361 acctcgaccg acccgatgga cttcgcgaag gtcatcgggc tgtgcgcgac gaccactgaa
     3421 caggacaccg ggtggggcgt catcagcgcc gagttcctgc tcccgatcgg caccgggacc
     3481 ccgcatgccc aaagccacgg catcttcccc tccttcggca ccgtgctgac cccgaaggcg
     3541 ggcgacagga tggcgatgct caccaccggg ttcgccgcca acccgttcga cagcgacaag
     3601 ttcggcggca aacagatggt ggcgcagagc agtgcgccgg ccgactggct cacgcttaac
     3661 ggcaatgtgt tcccggccgc gccggtctgc ggtggcgccg gcaccgccgg ctcggtcaac
     3721 gacccggtga tgttcaaact gcgggtccgg gtgcccgatg cggcgaagtc gttcagcgtc
     3781 aatgtctatt tcttctcgca ggaatatgtc aaatatgtct gctccgacta caacgatttc
     3841 ttcctgacgc tcctcgattc gacctatgtc gcgccggtcg agaaccccga actcgccaac
     3901 ccgaccgaca agaacctcgc gatggacggt gacggcaacc cggtcggcgt caatctcgcc
     3961 cccgccgggc tgttcaagca gtgcgtcaac gggacaagtg atgccgggac cgtcatcacc
     4021 acctgtgtgg acaccaacga actggcgggc accggtttca gttcctacgg cggcaccggt
     4081 tggctgacga cccgtggcaa tgtgaaggga ggcgaggtga tagaactgcg tttcgtgata
     4141 tgggacaccg gtccgtcggg cagtcccgac tatatctaca attcagccgt tctcatcgat
     4201 aacttccagt ggcagtacga ggaatacgag ccgggcacga tcgagtcata gggcatggaa
     4261 gacacccagc agaaaaaaag cgagaaacgg acgcgtaagc ggctcaagga tatctatgcg
     4321 caccatatcg tcgaggtgcc cggggaggag ccgcgcgaac ggctcggggc gctcatcagc
     4381 ctcggcaccc gcctgcgcga ccgcgaccgg cgtgagccgc tttccttcga ccggttcctg
     4441 cgcctcgcgg ccgaccggcc cgaactgatg ttccgcgaca tattccaact gttccacgat
     4501 atggtgcacc gctatgtccg gtcggggcgc gacgagcggt cgagtgaccc cgaatcggtc
     4561 gggttcgccg gctacgactg cgacgacctc ttcgtccggg ggcacgacaa ccccttcttt
     4621 gccgaccgcc tgttcgccaa ccggctcatg acgctcgtca acgggttcaa aaagggtctg
     4681 cgcgacaacc atatcatcct gttcgaaggc ccccccggca gcggcaagag cacctttctc
     4741 aacaccatcc tccagcgcct tgaggaatat gtccgcaccg cggagggaag ttacttcaag
     4801 acctactggc gcctgaacat cgagcggctc ggcggctacc agaatatcga gcggccgctc
     4861 cgccgcgccg ccgacggcgc cgaaagcccc gagatgcacg agcacctcga ccagatgcag
     4921 gatctcgaca cgcggttgcc gaagaagttc ctgaccttct cgtgtccgag cggcgaccac
     4981 ccgatactgc agataccgcg ttcgtaccgg cgcgaattcc tcgacgaact catcgcgaag
     5041 ggttcgttca aggacaaact gttccaccgc aaggagtacg agtgggtgtt caagggcatc
     5101 ccgtgcgcca tctgccggtc gctgtacgac atcctgcttg acatactcgg cgacccgctc
     5161 gaggtgttca acatgatctg tgcgcggcag atgcatttca accgccagtt cgggagcggc
     5221 atatccatct tcaacccggg cgatccggtg taccgtaagc cgataaccaa cccgcggctc
     5281 cagcggctca tcagcgacct cttgaagaac gacggcgtcg agttcatcta ttcggtgctt
     5341 gccaagacca acaacggcgt ttacgcgctc atggacatca aggagaacaa catcgagcgt
     5401 ctgcgcgacc tgcacggcat catcagcgac ggggtgcata aggtcgatct gaccgaggag
     5461 cgcgttaaat cgctgttcgt cgggctcgtg aaccccgagg acaaggtgca ctatcaggat
     5521 gtcaaatcgt tccaggaccg catcatcacc gtgcggatac cctacatact cgactacaac
     5581 accgaggtcg ccgtctaccg caacaagttc ggcaaggcga tagaccgcct gttcctgccc
     5641 ggggtactca ccaatgtcgc caagatagtg gtcgcctcgc ggctcgaaaa ggactcgccg
     5701 accatccgcg cgtggatagg cgataccgac cgttaccgga aatacatgga cgccgatgcg
     5761 cttctgctca aaatggacct ttacaccggg catatacccg attggctgtc ggaggaggat
     5821 gtccgcaagt tcgaccgcgc ggtgcgcacg gatctgctcg ccgagtcgga acaggagggg
     5881 ttcaagggct tctcggggcg tcagtcgctc aacatcttcg cacaactgct taccaactac
     5941 tgtgagccgg gcacgcccat cacgatggag atggtgaccc ggttcttccg gcagccgggc
     6001 gagccgctgt tcgacgagat accgggcgat ttcctcgatt cgatcgagga cctgtacgat
     6061 tacggggtac tgcaggaggt gaggacggcg ctctcgttct tcaacgagga tcagatatcg
     6121 cgcgatatcc agaactacct gttcgcgata aatttcgaca tcggcgcggt cgaaaagaat
     6181 gccgcgaccg gtgataccgt cgcggtgacc gaggagtatc tgcacggcct cgaggggata
     6241 ctgctcggcg ccgatgcgtc ggagcggcgc cgagccgagt tccgcgagga gacgcatgcc
     6301 gagtatgtca cggtgaccgt cacgcaggag atgaaggtgg aagggaagga actccgcgat
     6361 acggccctgt accggacact gttcgaccgc tacacccgca acctgcgcga gaatgccctc
     6421 gcgccgtacg ccgccaacga gaacttccgc cgcgcggtca ccgatctggg taccgccgct
     6481 ttttcagtgt acgacgagcg gatacagcgc gatgtgcgcg aactgatacg gatcctccgg
     6541 acaaaattca gatacacaca ggaaggggcg cgccagatcg ccctgtatgt catcgaaaag
     6601 aaactgcacg aaaaatactg agaaaagcgg ataacgcgct taccgctcta tgatgttcca
     6661 gtcgcgcatc tccaactgga agagcgattc gacgccgtcg cccgtcttgc ggtacccgaa
     6721 ggtgagccgc ccggtgacgg tgaccggtat cgtgtcggtg tagagttgtt cgcggtcgac
     6781 cttgagcggc ttgaggcccg acatcgtttc gacatggagg aggtcggcga aggtgggggg
     6841 gttgcagaag acgcagcccg ccatcacgac gatcgggttg gcgagccaga attccagcat
     6901 cgtgccatcg ttcgataccg gaccggtcgg catcacgatg cccgatatcg tcaccgccgc
     6961 gccgttcaac tgctggatca cctcctgcgg ccatttgtcg tggaggcgca ggtagaggtt
     7021 caccttgcgg agcccctgat aactgagatt gtagggcgac tcccagttcg gttcgacctg
     7081 cgcgaacttc gaccccgcct ttttgtaggc cttggcgtcg ccggcgcttt tcaccaccac
     7141 ctgccgcccg tgctcggtgt aacttttttt gacctggaat gcgccgtcgg tcgtttctcg
     7201 caggccggcg gcggggggcg cggtcagatc ggtcggtgcg ggaggggcca cctcgggagc
     7261 gggagaggag gccagttcct tactgaactt ctcggttatc gtgtcgggcc gcgccagttc
     7321 ctccttcacc gtttctcggt agttcaggta tgcgtggtaa cgccaaccga taaggatcgc
     7381 aacgatgagg acgatcgcgg cgagcatacc gttgaagagg tgttttttca tcggatcatc
     7441 ctttcgtgaa ggtgtggagg gtgcggacga taaagcggtc ggcgccgaag gtgacctcgc
     7501 tgacgagtcc ttcgtttttg agcgactcga cgatatccca cgaggcgccg gcggccgcga
     7561 ggatgctctc gatgttcgcg gtgcggagcg ggtggaccgc cgtgatggcg agtatgtcgg
     7621 cccgcgcgtc gccggtcgag gagaaggcgt tcccctcatg accggtgagc atctcggcgg
     7681 tgtccggcgg cagagcggcg ttgaatatct gccacgcggc ggcgagggcg tcgggggtcg
     7741 gcagaacgat gtcgagccgc gccgggggcc gcgtcgggac gaggatatag gcctttttcg
     7801 gcttcagcgc cgcgatgaat cgggccgtgg cggcaagatg cgcctgatcg tcgttgaccg
     7861 ccccgacgag catcgtctcg gtgagcaggg tccccgtg
//
LOCUS       DAOQUE010000070         7888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_10156_length_7888_cov_4.473637, whole genome shotgun sequence.
ACCESSION   DAOQUE010000070 DAOQUE010000000
VERSION     DAOQUE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10156_length_7888_cov_4.473637"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /gene="dinD"
                     /locus_tag="PKH10_03345"
     CDS             <1..504
                     /gene="dinD"
                     /locus_tag="PKH10_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HNT27193.1"
                     /translation="KRFEELTTEDEKRLFLRREMREHNKQLVEAAKNAGVIDPLDYAI
                     FQNHGYMGLYGGMTAKDIQRRKKLKEKDSILDHMGSTELAANLFRATQAEEKLKRDNI
                     KGKENANKTHFEVGKKVRKTIEELGGTMPEELPTPEKSVKELEKKGKKVKQLTARKKA
                     AKKPSKE"
     gene            726..1070
                     /locus_tag="PKH10_03350"
     CDS             726..1070
                     /locus_tag="PKH10_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27194.1"
                     /translation="MVKKIDFLTEYVRQEGARYYREISVDGGFWQVLSDTGLIAFSNL
                     GGFDETVFVLNLGKAEVTAHITVDKDLSAKAKFKDMSGGPQKTYPVKEQDGRCFVTIT
                     MKPGYGAVLVKK"
     gene            1170..2429
                     /gene="rocD"
                     /locus_tag="PKH10_03355"
     CDS             1170..2429
                     /gene="rocD"
                     /locus_tag="PKH10_03355"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNT27195.1"
                     /translation="MGCCCDKNCKEPKKLTSRELIEMEHHYGAHNYHPLEVVISRAEG
                     VWVWDPEGKKYLDCLSAYSAVNQGHRHPVIIKALKDQADVLTLTSRAFFNDRWPLFAK
                     KLCGYTGKDMVLPMNTGAEAVETAIKLTRRWGYEVKKVEKDKAEIIVMRENFHGRTTT
                     IVGFSTDPDAYNNYGPFTPGFVHVDYNDTGAVEKAITKNTVGVLVEPIQGEAGVFVPN
                     DGYMKKLREICTKHNVLLVADEVQTGFGRTGRKFCFMHDGIEPDIIIMGKALGGGVFP
                     VSAVAANKVIMGVFTPGSHGSTFGGNPLGCAVAMAALEVLDDEKLAERSAELGAYFMG
                     KLNELKTKTKKIKQVRGKGLLIGVVMDKDILSAAGEKARKYTTALKENGVLAKETHDW
                     IIRFAPPLVITKEEIDWAMGVISKVLV"
     gene            2438..3430
                     /gene="nagZ"
                     /locus_tag="PKH10_03360"
     CDS             2438..3430
                     /gene="nagZ"
                     /locus_tag="PKH10_03360"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNT27196.1"
                     /translation="MTSDLRHIKQAAGNALILSLAGPSLTPGEERFLREASPVGVILF
                     KRNIESLRSLELLCADLRRIDHLRIIAVDEEGGRVRRLPPGPYSLPSAQELAAMDDDA
                     FSEKVRALARELRRLGVTMDMAPVVDLRSGGEVSIVGDRAFSADPDEVARHAARYLSA
                     LRDEGILGVIKHFPGHGATTVDSHKELPRIQKPYADLAAEDLLPYRRLAGTARFVMAA
                     HILIPEIDAELPASISPAWGGILRRELGFDGILMTDDIEMHALDRWSPEEKTARFLAA
                     ESDILLVCSGTEEVMRAHWEALVYAAERGTATRRRLEKTGERVDGELFSLLGPR"
     gene            complement(3440..5290)
                     /locus_tag="PKH10_03365"
     CDS             complement(3440..5290)
                     /locus_tag="PKH10_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27197.1"
                     /translation="MLAGWSDAVSRIASLTERAPRSVLILFVFAATLIPRLYRAFTDM
                     GPVACDEIFQTIEVAHKLAFGRGFLYWEWSEGVRSYVLPGLLAGSYRLLDLVGVRDPY
                     MLAAGLKGVLALLHAGGWVFLFLFLAIFLTRFSALLVAVASGLLYAGIFASVRTLGET
                     VSMPFLLAALYFAARALDEDRGVRAVFAGLLAGIAFAIRFQTLFFSAALGLVLLLVAR
                     RRRAVVPFFVAGFAGILILTGLVDLFVWGGFLRSVVKYFDFNVLQDGASRFGRETWTY
                     YFTTFPKLYPWLLIVPALVVAVIGIFFRRTAMLVVPLAVYVLPHLMTPHKEVRFLFPV
                     YALFGLLALLGWERLSILAAPRHRPVAAVLAVAIALSSGLFHTRRIEPSWAQAHPLNN
                     AENMEPSFALGRIPDIKKGFVFDLQQDFSGGHAYFHANADIQYVRFIHEARPILFSEL
                     RRDLPGRYFAIRKRDEKHFAQYLPFLAKVAETTTWGIYRRDRMAMLEPEKISLARLTT
                     PTREGAAWDAPGTVRVWENGLRVSLDAVRNDGSLELSADNNDRYELSFIRGGMTVGYV
                     VAEPQRGKEGLVIHRLEVPAPAREQGYDEIVIMPSGGDGVFSVGHLLLER"
     gene            5420..6421
                     /locus_tag="PKH10_03370"
     CDS             5420..6421
                     /locus_tag="PKH10_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27198.1"
                     /translation="MADDRQERLFSLDREFLLFAPVYLALSYLNLAVKLWQTPAWFSL
                     LDRNHKLLLQFQYTNNEQSRIAQFYVPELFRQLFGLSVSHAYMLNRFLFLFLALFLFH
                     RYLRKWFSPAESFAGVLFLAAILPFTYMNCLQESAPQLFFLFVVALAAIRDERPFLLA
                     ATLFLGGMVNETMFILPAVYFFYHIRWERPVAMLQTVGRSLLVGVPIMLSLFPIRWFN
                     RDRPHLGGAWHWPDNWQGILRDAGRMFEAPHQAFYVYFVLLFGFFWLYAVLGYRRSPL
                     FLRRAFWLTPLFILAHLITGIIKESRQMVPLAYIIIPMALFFLLEKKGLAPTGRTDE"
     gene            6414..>7888
                     /locus_tag="PKH10_03375"
     CDS             6414..>7888
                     /locus_tag="PKH10_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27199.1"
                     /translation="MSDRPAIDRKTFVAFSFLVALLISFFKVYDDHDPDYVSPNERSR
                     FYAVHALAERGELRITPEFERFGRCLDIAKVGDEHYSDKAPLLALLAAPAYKLFLTVS
                     KDRAETEGTAYWFIKATTLAPFAVLLFWLICLFLLDRTGDRRFSLFAAFAALFATPVA
                     TYFGVLFSHALSATLLLATFLTARRATTAKSPPWFLATGALTGLTFLNEYQTAFALLL
                     MLILLLSRFRDTRKALWIIAGGLPFAALFFAYNTMLFGTPFSLGYGHLENKAFSEIHH
                     HGLYGITIPSGEAFVNLLFSPRIGFITLSPFLLFAFGSLYRPNGGPPRDRTTLDMLAV
                     AALHFLIIASFGFYGGGWAFGSRHLVAIVPFLTIPAALFVYERKSVLLHLAYLSLTLF
                     SVIIFTVANALRPLVEETIHNPLVNYYLLALRERYFVHSSLLSFLDVGNGASFLLYFA
                     VTIGLFLFLAYRAVPGPIRATVFFPAPLVACFFCTLLMLPYR"
BASE COUNT         1646 a         2582 c         2184 g         1476 t
ORIGIN      
        1 aaacggtttg aagaactcac caccgaagac gaaaaacgcc ttttcctgcg ccgcgaaatg
       61 cgggaacaca acaagcaact cgtcgaagcg gcgaagaacg cgggcgtcat cgatccgctc
      121 gactatgcca tcttccagaa ccacggatac atgggcctct acggcggcat gacggcgaaa
      181 gacatccagc gtcgcaagaa actgaaagag aaagacagca tccttgacca catgggaagt
      241 accgaactgg ccgccaatct attccgcgcg acgcaggccg aagagaagtt gaagcgcgac
      301 aacatcaagg gaaaagagaa cgccaacaag acccacttcg aggtcgggaa aaaagtgcgc
      361 aagaccattg aagaacttgg cgggaccatg ccggaagaac tgccgacacc ggagaaaagc
      421 gtcaaagaac tcgaaaagaa gggaaagaag gtcaagcaac tcacggcacg aaaaaaggcc
      481 gcgaaaaaac cgagcaagga atagtcacca tgcccggccg tgatgcggta tctaccgtcc
      541 gcgaacctga tatcgcccgt tttttcttct ttttttcccc tccgccgtta caacagataa
      601 ccataataaa aacaaacgac taactacctc ctcataaaaa attcattgac aatcggggct
      661 tcccccctac aatgcctccg ctatccgttt tttagtaacc gaatgtatca ccaaggggga
      721 acgacatggt gaagaagatc gattttttga ccgagtatgt gcgacaggag ggggcccgtt
      781 attaccgtga gatatcggtg gacggcgggt tctggcaggt actcagcgac accgggctca
      841 tcgccttttc gaacctcggc gggttcgacg aaacggtatt cgtgctcaat ctcggcaagg
      901 cggaggtcac ggcgcatatc accgtcgaca aggacctgtc ggccaaggcg aagttcaagg
      961 acatgagcgg tggcccgcag aagacctatc cggtcaagga acaggacggt cgctgtttcg
     1021 tcaccatcac gatgaagccg ggctacggcg cggtgctcgt caagaaatag ccggtcttcc
     1081 cgtaccttcc caaaattctt gccttaaacg ggttccctgc tagactcgcc tcgttccgtg
     1141 ttcaataact atttgaaagg agccgagtca tgggatgttg ctgcgacaag aactgcaagg
     1201 aaccgaagaa actgacctcg cgtgaactca tcgagatgga gcaccactac ggtgcgcaca
     1261 actatcatcc gctcgaagtg gtcatcagcc gtgccgaggg cgtgtgggtc tgggacccgg
     1321 aaggaaagaa atatctcgac tgtctgtcgg cctattcggc ggtcaatcag ggacaccgcc
     1381 acccggtcat catcaaggca ctcaaggatc aggccgatgt gctgacgctc acgagccgcg
     1441 ccttcttcaa cgaccgctgg ccgctgttcg cgaagaaact ctgcggatac accggcaagg
     1501 acatggtcct gccgatgaac accggcgccg aggcggtcga gaccgccatc aaactcaccc
     1561 gccgctgggg ttacgaggtc aagaaggtcg agaaggacaa ggccgagatc atcgtcatgc
     1621 gcgagaattt tcacggccgc accaccacca tcgtcggctt ctcgaccgat cccgacgcct
     1681 acaacaacta tggccccttc acgcccggct tcgtccatgt cgactacaat gacaccggcg
     1741 cggtcgaaaa ggcgataacg aagaacaccg tcggcgtact cgtggagccc attcagggcg
     1801 aggcgggcgt ctttgtcccg aacgacggct acatgaagaa actgcgcgag atatgcacca
     1861 agcataatgt cctgctcgtc gccgacgagg tacagaccgg cttcggccgc accggcagga
     1921 aattctgctt catgcacgac ggcatcgaac ccgatatcat catcatgggt aaggcgctcg
     1981 gcggcggcgt gttccccgtt tcggcggtcg cggcgaacaa ggtcatcatg ggcgtcttca
     2041 cccccggctc gcacggatcg acattcggcg gcaacccgct cggttgcgcc gtggcgatgg
     2101 cggcgcttga ggtgcttgac gacgagaaac tggccgaacg gtcggccgaa ctcggcgcct
     2161 atttcatggg caaactgaac gaactgaaga caaagaccaa gaagataaag caggtccgcg
     2221 gcaagggact cctcatcggg gtcgtgatgg acaaggatat cctttccgcc gccggcgaaa
     2281 aggcacgcaa gtacaccacc gccctcaagg agaacggcgt attggcgaaa gagacccacg
     2341 actggatcat ccgcttcgcg ccgccgctcg tcatcaccaa agaagagata gactgggcga
     2401 tgggcgtcat ctcgaaggtg ctcgtctagg caccccgatg acgagcgacc tgcggcacat
     2461 caagcaggcg gcgggcaacg cgctcatcct ttcgctcgcc gggccatcgc tcacacccgg
     2521 cgaagagcgg ttcctgcgtg aggcatcgcc ggtcggcgtc atcctcttca aacgcaacat
     2581 tgaatcgctc cggtcgctcg aactgctctg cgccgacctg cgtcgcatcg accatcttcg
     2641 catcatcgcg gtcgacgagg agggaggccg cgttcggcgc ctccctcccg gtccctactc
     2701 cctgccgtcg gcccaagaac tcgccgcaat ggacgacgat gcgttcagcg aaaaggtccg
     2761 ggcactcgcc cgtgaactgc ggcgactcgg cgtcacgatg gacatggcac cggtggtcga
     2821 cctgcgttcc ggcggggagg tgagcatcgt cggcgaccgc gccttttccg ctgaccccga
     2881 cgaggtggca cgccacgcgg cccggtacct ttcggcgctc cgcgacgagg ggatactcgg
     2941 cgtcatcaag catttccccg gtcacggcgc gacgacggtc gattcgcaca aagaattgcc
     3001 gcggatacaa aaaccgtatg ccgacctcgc agcggaggac ctgctcccct accgtcggct
     3061 cgccgggacg gcgcggttcg tcatggcggc ccacatactc atcccggaga tagatgccga
     3121 actgcccgcc agcatctctc ccgcgtgggg cggcatcctg cgacgcgaac tcggctttga
     3181 cggcatcctc atgaccgacg acatcgagat gcacgcgctc gaccgctggt cacccgagga
     3241 gaagacggca cgtttcctcg ccgccgagag cgacatcctg ctcgtctgct cgggaaccga
     3301 agaggtgatg cgggcccact gggaagcgtt ggtgtatgcc gccgaaaggg ggacggcgac
     3361 ccggaggcgg ctcgaaaaga ccggcgaacg ggtggatggc gaactattct ctcttctcgg
     3421 accgcgctga ccggcggcgc tacctttcca gcaacaggtg accgacgctg aaaaccccgt
     3481 cgcctcccga cggcataatg actatctcgt cgtagccctg ttcccgcgcc ggagcgggga
     3541 cctcgagccg gtggatgacg agcccctcct tgccccgttg cggctcggcg acaacgtagc
     3601 cgacggtcat gccaccacgg atgaacgata actcgtaacg gtcgttgttg tcggccgaca
     3661 gttccaacga accatcgttc cgtaccgcgt caagcgacac ccgcaggccg ttctcccaga
     3721 cccgcaccgt acccggggca tcccatgcgg ccccctcacg cgtgggggtc gtgagccgcg
     3781 caagcgatat cttttctggc tcaagcatcg ccatccggtc ccggcggtag ataccccacg
     3841 tcgttgtttc ggcgaccttc gcaaggaacg ggagatactg cgcgaagtgc ttctcatccc
     3901 ttttccgtat cgcgaaataa cgacccggga gatcgcggcg cagttcggaa aagaggatcg
     3961 ggcgcgcctc gtggatgaac cggacatact ggatgtctgc gttcgcgtgg aaataggcgt
     4021 ggccacccga gaagtcctgt tgcagatcaa acacaaaacc ttttttgatg tcgggaatgc
     4081 gaccgagcgc gaacgacggc tccatgttct cggcgttgtt cagcggatgg gcctgcgccc
     4141 acgacggctc gatgcgacgg gtatggaaca gtcccgagga gagggcgatg gccaccgcga
     4201 gcacggcggc gaccggccgg tggcgcggcg cggcaagtat cgagagccgt tcccatccaa
     4261 gtaacgccag aaggccgaag agagcgtaga ccgggaacag gaaacggacc tccttgtgcg
     4321 gggtcataag atgcggcagg acatagaccg cgagcggcac gaccaacatc gccgtacgac
     4381 ggaagaatat cccgatgacc gccacgacaa gcgcgggaac gatgaggagc cacggataca
     4441 gtttcgggaa agtggtgaaa tagtatgtcc atgtttcgcg accgaaccgt gatgcgccgt
     4501 cctgcaacac attgaagtcg aagtatttca cgaccgaccg gagaaatccc ccccagacga
     4561 acaggtcgac aagcccggtg agtataagta tccccgcgaa cccggccacg aagaacggga
     4621 ccaccgcccg gcgacggcgc gcgacaagca ggagcacaag accgagcgcc gccgagaaaa
     4681 aaagcgtctg aaaacggatg gcaaaggcga tacccgccag aagaccggcg aacacggctc
     4741 tcacgccgcg atcctcgtcg agagcgcgag cggcgaagta gagcgccgcc aacaggaacg
     4801 gcatcgacac cgtctcgccg agcgttcgca ccgacgcaaa gatgcccgca tacaacagtc
     4861 cggaggcgac ggcgacaaga agtgcactga accgcgtgag gaagatcgcg agaaagagga
     4921 acaggaatac ccagccaccg gcatgcagaa gcgcgagaac ccccttcagt cccgccgcga
     4981 gcatatacgg atcgcgcacc ccgaccagat cgagcaggcg atacgacccg gcgagaagcc
     5041 ccggcaggac ataggatcga accccttccg accactccca atacaggaag ccgcgcccga
     5101 aggccagctt gtgggccact tcgatcgtct ggaatatctc gtcacacgcg accgggccca
     5161 tgtcggtgaa cgcacggtag agccgcggga tgagcgtcgc cgcgaacacg aaaaggatga
     5221 ggaccgaccg cggggcgcgc tccgtaagcg aggcgatgcg cgacaccgcg tccgaccatc
     5281 ccgccagcat tttcgacacg gagcacctcg gccgaaagga aaaagaacac cctctaggtt
     5341 ctagttcctt tccttgacaa gtcaaaataa atcggtcaga taggtccggc gccgcaacgc
     5401 gtttcacggg gaccgacgca tggccgatga ccgtcaggaa agacttttct ccctcgatcg
     5461 ggagttcctg ctcttcgccc cggtctatct cgccctttcc tacctcaacc tcgcggtaaa
     5521 actctggcag acccccgcgt ggttctctct gctcgaccgc aaccacaaac tcctcctgca
     5581 gttccagtac acgaacaacg aacagtcgcg catcgcccag ttctatgtcc ccgaactgtt
     5641 ccgccaactg ttcgggctct cggtatccca cgcctacatg ctgaaccgct tcctcttcct
     5701 gttcctcgct cttttcctct tccaccgata cctgcggaaa tggttctctc ccgccgagag
     5761 tttcgccggg gtcctgttcc tcgccgcgat acttcccttc acctacatga actgcctgca
     5821 ggagagcgcg ccgcaactat tcttcctgtt cgtcgtcgcg ctcgccgcca tccgtgacga
     5881 gcgaccgttc ctgcttgccg caacactgtt cctcggcggc atggtgaacg agacgatgtt
     5941 catcctgccg gcggtttatt tcttctatca tatccgatgg gagcgcccgg tcgcgatgct
     6001 gcagaccgtc ggacggtcgc tcctggtcgg ggtgccgatc atgctctcgc tcttcccgat
     6061 ccggtggttc aatcgcgatc gcccccacct cggcggtgcg tggcattggc ccgacaactg
     6121 gcaaggcatc ctgcgcgacg ccggtcgcat gttcgaggcg ccccatcagg cgttctatgt
     6181 ttacttcgtc ctgctgttcg gctttttctg gctctacgct gtcctcgggt accgacggag
     6241 ccctctgttc ctgcggcgtg ccttctggct gacgccgctg ttcatactcg cccatctcat
     6301 cacgggcatc atcaaggagt cacgccagat ggtgccgctc gcctacatca tcatcccgat
     6361 ggcgctcttt ttcctacttg aaaaaaaggg cttggcgccg accggtcgca ccgatgagtg
     6421 accgtcccgc catcgaccgg aagaccttcg tcgccttctc gttcctcgta gccctgctca
     6481 tctctttctt caaggtctat gacgaccacg atcccgacta tgtgagtccc aacgagcggt
     6541 ctcgtttcta cgccgtccac gcgctcgccg aacggggcga actgcgcatc actcccgaat
     6601 tcgaacggtt cggtcgctgt ctcgatatcg cgaaggtagg cgacgaacac tactccgaca
     6661 aggcgcctct gctcgccctt ctcgctgccc ccgcctacaa actctttctg accgtttcga
     6721 aagatcgcgc cgagacggag gggaccgcct actggttcat caaggcgacg acgctcgccc
     6781 cctttgcggt actgctcttc tggctgatat gcctcttcct gctcgaccgg accggcgacc
     6841 ggcgtttttc gctctttgcc gcctttgccg ctttgttcgc cacgccggtg gcgacctatt
     6901 tcggcgtact gttctcccat gcgctctcgg cgacgctcct tctcgccact ttcctcaccg
     6961 cccgccgagc cacgaccgcg aaaagcccgc catggttcct cgcgaccggt gcgctgaccg
     7021 ggctgacctt tctcaacgaa taccagaccg cattcgccct cctgctcatg ttgatcctgc
     7081 tgctctcccg tttccgcgac acgcgaaagg ccctctggat catcgcgggc ggactcccgt
     7141 tcgccgcgct ctttttcgcc tacaacacga tgctgttcgg cacgccgttc tcgctcggct
     7201 acggccatct tgagaacaag gccttttcgg agatacatca ccacgggctc tacggcataa
     7261 cgatcccctc gggagaggcc ttcgtcaacc tgctcttttc cccgcgcatc ggctttatca
     7321 ctctgtcgcc tttcctgctg ttcgcattcg gatccctgta ccgcccgaac gggggaccgc
     7381 cccgcgaccg cacgacgctc gacatgctcg cggtggccgc cctacatttc ctcatcatcg
     7441 cctcgttcgg cttctatgga ggcggctggg ccttcggatc gcgccacctc gtcgctatcg
     7501 taccctttct caccataccc gcggcactgt tcgtgtacga aagaaaaagc gtcctcctcc
     7561 accttgccta cctctcgctc accctcttct cggtcatcat cttcacggtg gccaacgccc
     7621 tgcgaccgct cgtcgaggag actatccata atccgctcgt aaattactac ctcctagccc
     7681 tgcgcgaacg ctacttcgta cattcctccc tcctatcttt tctggatgtc ggtaacggcg
     7741 catcctttct cctctacttt gccgtgacca tcggtctctt ccttttcctt gcctaccgcg
     7801 ccgttcccgg gccgatcagg gcgaccgtct ttttcccggc gccgctcgtc gcttgtttct
     7861 tctgcacgct tctcatgctc ccctaccg
//
LOCUS       DAOQUE010000071         7740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_10402_length_7740_cov_4.119974, whole genome shotgun sequence.
ACCESSION   DAOQUE010000071 DAOQUE010000000
VERSION     DAOQUE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10402_length_7740_cov_4.119974"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PKH10_03380"
     CDS             <1..592
                     /locus_tag="PKH10_03380"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNT27200.1"
                     /translation="IEQTEALTVVDVNTGKFLGHGSLEQTVLRTNLEAVDEIAYQLRL
                     RNIAGIIVVDFIDMRHHANRQKVIRALETALEHDPAKNTVYQFTRLGLIQITRKRTSE
                     SALATLTEPCPYCTGSASILSRESVCFKIIRELVRQNRLYQTRRFHVEAHPDVVFALQ
                     QTFADEFAKVQKTHRLMVEVRSDGSLHREHFVVSAD"
     gene            complement(612..4289)
                     /locus_tag="PKH10_03385"
     CDS             complement(612..4289)
                     /locus_tag="PKH10_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27201.1"
                     /translation="MPLLTQATSVIDGFDPSVYGNVYAVALQVDGKILIGGDFTEAGG
                     ETRNNIARLNPDGTIDETFDPDANNEVRAIAVQTDGKILLGGTFTTIGGVARNRIARL
                     NGDGSLDTSFDPNANSEVLALAIRPDGKIMAGGGFTSIAGTAKQYLAQLNADGSVDIS
                     FEPPNIIAFVLSIVVQNDGKILAGGLHWGAGTRAVVRLNLDGSVDEAFTYSCSPNDDV
                     YALAVEPDGQILVGGVFSLLGGEEREHIGRVDTDGNADRFFDPNADDPILTIAMQADG
                     KILVGGVFTSVGGIARNNIARLNADGTVDESFDPEVAFGEVYAFAVQADGKILVGGIG
                     GIVRLNADGTLDANLNPDANGIVNGLALQVDGKILVGGEFTTIGGETKNHIARLNADG
                     TLDATFAPSVNSTVYAIALQSDGNMLLAGLFTSIGGTTRNRLARLNTDGSLDTAFDPN
                     ANDIVFGALVQPDGKIVVCGSFTTIGGITRNRLARLNADGSLDTTFDIGADNYISALA
                     LQSDGKILVGGAFATIGGQARSRIARVNADGTLDTVFSADASSFVRSIAVQADGKILV
                     GGAFSTIGGVARNYIARLHTDGSVDTVFNPGANGQVYPIVVRTNGKIVAGGNFTTLGG
                     MTRNHIAQLNSDGSLDTAFDPNADSYVWALAIQADGKVVAGGDFTTIGGETRNRLARL
                     SADEAALLEIAVSSDGTTVQWIRSGSGPEVHEVFFEGSSDGSTWSFLGVGTRISGGWE
                     LGGLSLPFYQQYQVRVRGKTYGGYMNDSTSFIESTRLFYLTCDIDHIYEGGACINSKE
                     VPCDEDNANPVNSSDTIANVTIHYTTDGGWELPADCAWTCDDGFFLNGESTACVYCLV
                     DGDCADGNACNGEELCDDLGLCNPGEPLVCADDNACTDDSCDPSAGCQYIDDDTNTCD
                     DGNDLTENDVCFEGDCAGTLMSGVCGNAIVIGALPYTTTGDTVTRPNSLETYGVGCAA
                     ATQATVDMVYELTVEEGIEYQIQVVPTDGGDLALNLLGVCGAGEACIDAANGGGAGET
                     EVIVGVAGQSGTVYIAIEGSGAYELSIGIVEQPDDDTVEPDDIIEPDEDAIGQDEVFT
                     DEVTLDDVVTDDILTDDILIDEEETDDAPVILDDGTPLADDTVTPDDGMPQDKDTPLP
                     DTDIDSVMPHCGDGTTNGPEFCDDGSLLNGTCGHCNATCTGYTACDDADNLLDDEDSS
                     LPDDIDTDKPKDEGCGCSLVF"
     gene            complement(4481..6319)
                     /locus_tag="PKH10_03390"
     CDS             complement(4481..6319)
                     /locus_tag="PKH10_03390"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27202.1"
                     /translation="MKRVFFVLFLVALVPCLLLALPTTFNLTVTKSGSGDGVLTDGLD
                     HLDCGSVCTYDYDTGTTVTVTATADPLSHFVEWSGACTGTLTTCNVTMDTPQTLDAKF
                     ACDDGNIRLLGTDCGKNGNGVAYEECVTGDWEEKCNDSDLCVAGEQYCDSGDLVLCEL
                     QDGGIYDWSTIDCDDDNECTNDVCDDVFGCVNGPTAEFTPCGDQTDTDCDGPNYCDGD
                     GACLDNFEVKDTPCGDNTDTVCDKPDTCDDAGQCLDNFEPDTTPCRDREIGHLCDAIE
                     YCDGFGGPCPEDKYLTANDICRPSIGTCDAPEKCDGYSDICPPEDYTATNGISCEDVY
                     DYTDEETCANGECLGTEIIGSCSDAYEATIFPYVLESTTVGRPSHLTTYGTNCPTNNA
                     PLGDVVVHVDMDGGVEYTISIERHGGWTGFIAIIPICSVIFTNATCLNTGGDTDSFTY
                     TPLIGGNATLVVESLSGTGDFTLTIEREETPQPDIDTVTPDDLLLDDTVTDEEEPDEV
                     VIDDVVTDEAMTDDILIDEEETDGTAIDEGEEEDIDDPPTGEGSEPDWAEAKPDVDTT
                     VADTTVVDNEIPDETGDELLGDEDAFIPGADTDKPVDGGCGCSLVF"
     gene            complement(6391..7527)
                     /locus_tag="PKH10_03395"
     CDS             complement(6391..7527)
                     /locus_tag="PKH10_03395"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNT27203.1"
                     /translation="MNDFIQKELDELAAAGLKRELKDFVPDGSALRGEFRGRRVVNFS
                     SNSYLGLDSHPALKKAAIAALEKWPVGASASRLITGTGPWVKELEERTAAWKKREAAL
                     YFGSGYAANVGIIAALADRDTDIFLDKLDHASIVDGAILSRAKIHRYRHNDIADLERL
                     LKRSTAARKLLVTDTIFSMDGDIAPLEDIVGLCRKYEALLIVDEAHASGVYGATGGGL
                     MEERGLAGKAFLEMGTFSKGLGAAGAYVAGDRATIDYLINRARSFIFTTATPPMVCAA
                     CAAAIDIVRSDEGTRLRERLQRHIATLRTVVPAAATPIVPIVLGDIPATMARYETLLE
                     KGLLGLPVRPPTVPEGTARLRLSLSAGHTTEEMAALVGALSTIN"
     gene            complement(7524..>7740)
                     /locus_tag="PKH10_03400"
     CDS             complement(7524..>7740)
                     /locus_tag="PKH10_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27204.1"
                     /translation="AGVVVSGYAPGEETADRNILHLPRLCNAPLLGVLPRILGLDTER
                     PGWLTPEAADALQERFDWPAILAACGA"
BASE COUNT         1504 a         2339 c         2279 g         1618 t
ORIGIN      
        1 catcgaacag accgaggcgc tcacggtggt cgacgtcaac accggcaaat tcctcggcca
       61 cggttcgctc gaacagacgg tgctgcggac caacctcgag gcggtcgacg agatcgccta
      121 tcaactgcgc ctgcgcaaca tcgccggtat catcgtggtc gacttcattg acatgcgtca
      181 ccacgccaac cgtcagaagg tcatccgggc gcttgaaacg gcgctcgaac acgatccggc
      241 caagaacacc gtctatcagt tcacgcgact cgggcttatc cagataacac gcaaacggac
      301 ctccgaaagc gcgcttgcca cgctcaccga gccgtgtccc tactgcaccg gctccgccag
      361 catactgtca cgcgagtcgg tctgcttcaa gatcatccgc gaactggtgc ggcagaaccg
      421 cctgtaccag acgcgccgct tccatgtcga ggcgcacccc gatgtcgtct tcgcgctcca
      481 gcagaccttc gccgatgaat tcgcgaaggt gcagaagacc caccggctca tggtcgaggt
      541 gcggagcgac gggtctctcc atcgggaaca tttcgtcgtg tcggccgact gatccgccgg
      601 ccgttatctc tctagaacac cagtgaacag ccgcaacctt cgtctttcgg cttgtcggtg
      661 tcgatgtcgt cgggaagaga ggagtcctca tcgtccaaaa ggttgtccgc gtcgtcgcac
      721 gcagtgtacc cggtgcaggt cgcattgcag tgtccgcagg tcccgttaag gagagagccg
      781 tcatcgcaga actccggtcc attggtcgtg ccatcaccgc agtggggcat gaccgagtcg
      841 atgtcggtgt cgggaagagg cgtatccttg tcctgcggca tcccgtcgtc gggggtcacg
      901 gtgtcatccg cgagcggggt gccgtcgtcg aggatgaccg gcgcgtcgtc ggtctcttcc
      961 tcgtcgatga gaatgtcgtc ggtgaggata tcgtcggtca ctacatcgtc aagcgtcacc
     1021 tcatcagtga aaacttcgtc ctgcccgatg gcatcctcat ccggttcgat gatgtcgtcg
     1081 ggttccaccg tgtcgtcatc gggctgttcc acgatgccga tcgacagttc atatgcgccg
     1141 ctaccttcta tcgcgatgta gaccgtgccg ctctgaccgg caacgccaac gatcacctcg
     1201 gtctcgcccg caccgccgcc gttcgccgcg tcgatgcatg cctcgcccgc gccgcagaca
     1261 ccgagcagat tcagggcaag atctccacca tcggtcggca ccacctgtat ctggtattcg
     1321 atgccctctt cgaccgtcag ttcgtacacc atatcgacgg tcgcctgcgt ggcggccgcg
     1381 cacccgacgc cataagtttc aagtgaattc gggcgagtaa cggtgtcgcc ggtcgtcgtg
     1441 tagggcaggg cgcctatcac gatcgcattg ccgcacactc cgctcataag cgttcccgcg
     1501 cagtcgcctt cgaagcagac atcgttctcg gtcaggtcgt tcccgtcatc gcaggtgttc
     1561 gtatcatcat cgatgtactg gcatccggcg gacggatcac acgagtcatc agtacaggca
     1621 ttgtcgtcag cgcagaccag cggttcaccc gggttgcaga ggcccagatc atcgcacaat
     1681 tcttcaccgt tgcaggcatt tccgtcggca caatcgccgt ccacgagaca gtaaacacag
     1741 gcggtcgatt cgccgttgag gaagaagccg tcatcgcagg tccacgcaca gtcggcgggt
     1801 agttcccatc caccgtcggt ggtgtagtgg atcgtgacat tggcgatggt gtcgctactg
     1861 ttcactggat tggcgttgtc ttcatcgcac ggcacttcct tcgagttgat gcaggcgccg
     1921 ccctcgtaaa tatgatcgat gtcacaggtg agataaaata accgcgtcga ctctatgaac
     1981 gatgttgaat cgttcatata tcccccgtat gtcttaccgc gtacccttac ctggtactgt
     2041 tggtaaaagg ggagggaaag cccgccaagt tcccagccac ccgatatcct agtgcctacg
     2101 ccgagaaatg accatgtgga cccgtcggaa gacccttcaa aaaagacctc atgcacctcc
     2161 ggtcccgagc cgctccttat ccattgcacg gttgttccat ctgaagagac cgcaatctcc
     2221 agcagagccg cctcatcggc gctcaaacgg gctaagcggt ttctcgtttc tcctccgatg
     2281 gtggtgaaat cgccacccgc tacgacctta ccgtcagcct gtatcgcaag agcccagaca
     2341 taggagtcgg cattcgggtc gaacgcggta tctagtgatc cgtcagagtt gagttgggct
     2401 atgtggttcc gggtcatacc tccgagcgtg gtaaagttgc cgcctgcgac gatcttcccg
     2461 ttcgttctaa caactatggg gtaaacctgt ccatttgcgc cggggttgaa aaccgtatct
     2521 acggagccgt ccgtgtggag ccgggcgatg tagtttcgcg ccacacctcc tatggttgaa
     2581 aaggcgccac ccacgagtat cttgccatcc gcctgtaccg ctatggaacg aacaaagcta
     2641 cttgcgtctg cgctaaaaac cgtatccagg gtgccgtccg cgttcacgcg ggcgatacgg
     2701 ctccgggcct gtccgccgat cgttgcgaag gcgccgccca ccagtatctt tccatcggat
     2761 tgcaacgcca gagcactgat atagttgtcc gcaccgatat caaaggtcgt gtccagtgag
     2821 ccgtcggcat taagacgggc caatcgattc cgcgtgatgc caccgatggt ggtgaaagag
     2881 ccacagacaa cgatctttcc gtcgggttga acgagcgctc cgaacacgat gtcgttcgca
     2941 ttcggatcga aagccgtatc caacgacccg tcagtattga gacgggccaa ccgattccgc
     3001 gtcgtacctc cgatagaggt aaatagccct gccaaaagca tgttaccgtc ggactgcagg
     3061 gcaatcgcgt aaaccgtgct gttcaccgac ggggcgaagg tcgcgtcgag agtgccgtcg
     3121 gcattgagcc gggcgatatg gttctttgtc tcgccaccga tagtagtgaa ctcgcccccc
     3181 accagtatct tgccatcaac ttgcagggcc aatccattga caatgccatt tgcatcagga
     3241 ttcaagttcg catcaagtgt gccgtcggca ttgagacgga cgatacctcc gatgcctccc
     3301 acgagtattt tgccatcggc ctgtacggca aaggcataga cctctccaaa ggccacttcg
     3361 ggatcaaagc tctcgtccac cgtgccgtcg gcattgaggc gggcgatatt gttccgggct
     3421 atgccgccca cagaggtgaa aacgccaccg accagtatct taccgtcggc ctgcatggct
     3481 atggttaaga tggggtcatc cgcattcggg tcgaagaagc gatctgcatt accatcggtg
     3541 tcaacccgac cgatgtgttc gcgttcttcc ccgcccaaca aggaaaacac tcccccgacc
     3601 agtatctgtc cgtccggttc taccgcaagg gcatagacat cgtcgttcgg gctacaggaa
     3661 taggtaaagg cttcgtccac ggagccgtca aggtttaatc ggactacagc ccgcgttccc
     3721 gccccccaat ggagccctcc tgctaggatc ttgccgtcat tttgtaccac gatagacagc
     3781 acaaaggcaa taatgttcgg gggctcgaaa ctgatgtcca ccgagccgtc agcgttgagt
     3841 tgtgccagat actgttttgc ggtaccggct atactggtaa atccgccgcc ggccattatc
     3901 tttccatcgg gtcgtatcgc cagagcgaga acttcactgt tggcattcgg gtcgaaggag
     3961 gtatccaaag aaccgtcccc gttgagacgg gcgatgcggt tccgcgccac cccgccgatg
     4021 gtggtgaacg taccccccag cagtatcttt ccgtctgttt gcacggcgat agcgcgaact
     4081 tcattattcg catcggggtc aaaagtttca tcgatggttc cgtcggggtt aaggcgagcg
     4141 atattgttcc gtgtctcgcc gccagcctcg gtaaaatctc ccccgatcag tatcttgcca
     4201 tcaacctgta gagcaacggc ataaacattg ccgtacacag acggatcaaa gccgtcaatg
     4261 accgaggtcg cctgagtcaa gagcggcatc aggaaaatca cgcagaaaaa aagagcggaa
     4321 aaaaaacctt tagccctgaa aaattccatt accgaacctc ctgtgtcggc actattgggg
     4381 atgacgagtc ttttatgttt cataacggag gaaaggatat cacctttctg aaaaaaaaca
     4441 accgtggtga aggtgtaaag agaaaatgag gaaagggtat ctagaacacg agcgaacaac
     4501 cacagccgcc gtcgaccggt ttgtcggtgt cggcgccggg gatgaaggca tcttcatcgc
     4561 cgagcagttc gtcgcccgtt tcgtccggta tctcgttgtc cacgacggtg gtatcggcga
     4621 ccgtcgtgtc gacatccggc ttggcctcgg cccaatcggg ctccgaacct tcgccggtcg
     4681 gcggatcgtc aatgtcttcc tcttcccctt cgtcgatggc cgttccgtcg gtctcttctt
     4741 cgtcgatgag gatgtcgtcg gtcatcgcct cgtcggtcac cacatcgtcg atgaccactt
     4801 cgtccggttc ctcctcgtcg gtgacggtgt cgtcgagcag aagatcgtca ggtgtcaccg
     4861 tgtcgatgtc gggctgaggc gtctcctcgc gctcgatggt cagcgtgaag tcaccggtgc
     4921 cgctgagcga ctcgaccacg agcgtcgcgt tgccgccgat gagcggcgta taggtgaacg
     4981 aatcggtgtc gccgccggtg ttgaggcagg tggcgttggt gaagatgacg ctacagatgg
     5041 ggatgatggc gatgaagccg gtccagccgc cgtggcgttc gatcgatatc gtgtattcga
     5101 ccccgccgtc catgtcgaca tgaacgacga catcgccgag cggcgcatta ttcgtcggac
     5161 agttggtgcc gtaggtggtc aggtggcttg gccgcccgac ggtggtgctt tcgagcacat
     5221 aggggaatat cgtcgcttcg taggcatcgg agcaggagcc gatgatctcg gtgccgaggc
     5281 attcgccgtt ggcgcaggtc tcttcgtcgg tgtaatcgta gacatcctcg caggagatac
     5341 cgttggtcgc ggtgtagtcc tcgggaggac agatgtccga atacccgtca catttttcgg
     5401 gggcatcgca ggtgccgatc gaggggcggc agatgtcatt cgccgtcagg tatttgtcct
     5461 cggggcaggg gccgccgaag ccgtcgcagt attcgatggc gtcgcagagg tggccgatct
     5521 cccggtcgcg acagggggtg gtgtcgggct cgaagttgtc gaggcactga cccgcgtcgt
     5581 cacaggtgtc gggtttgtcg cacacggtgt cggtattgtc gccgcacgga gtgtccttga
     5641 cctcgaagtt gtcgagacat gcgccgtcgc cgtcgcagta gttcggtccg tcgcagtcgg
     5701 tatcggtctg atcgccgcag ggggtgaact cggcggtggg gccgttcacg caaccgaaca
     5761 catcgtcgca cacatcgttg gtgcattcgt tatcgtcgtc gcaatcgatg gtcgaccagt
     5821 cgtatatgcc gccatcctgc agttcgcaca acaccagatc accgctgtcg cagtactgtt
     5881 cgccggcgac gcagagatcg gaatcgttgc atttctcctc ccagtccccc gtgacgcatt
     5941 cctcgtaggc gacgccgttc ccattcttcc cgcaatcagt gccgagcaac cggatattgc
     6001 catcgtcgca ggcgaacttt gcgtcaaggg tctggggggt atccatggtc acattgcagg
     6061 tcgtcaaggt gccggtgcag gcgccgctcc attcgacgaa atggctgagg ggatcggcgg
     6121 tcgcggtcac tgtcaccgtc gtgccggtgt cgtagtcgta ggtgcagacg ctgccgcagt
     6181 caagatggtc cagaccgtcg gtcagaacgc cgtcacccga accgctcttg gtgacggtca
     6241 gattgaaggt ggtgggcagg gccagcaaca gacacggaac gagcgcgacg aggaaaagaa
     6301 caaaaaacac ccttttcatg aacatcctcc ctttctctac ggataaaaaa catcttaatg
     6361 ggtcgatgct aataaagaac ggatcggatg tcaatttatc gttgacagag caccgaccag
     6421 cgccgccatc tcttcggtgg tatgtcccgc cgaaagcgac agtcgcagcc gcgcggtccc
     6481 ttcgggcacc gtggggggcc tgaccggcag accgagaagc cctttttcga ggagggtttc
     6541 gtagcgcgcc atcgtcgccg ggatatcacc cagcacgatc ggcacgatcg gcgttgcggc
     6601 ggccgggacg accgtgcgga gcgtcgcaat gtgacgctgc agccgttcgc ggagtcgcgt
     6661 tccctcgtcc gacctaacga tatcgatggc ggccgcgcag gcggcgcaga ccatcggcgg
     6721 ggtcgcggtg gtgaagatga aactgcgggc gcggttgatg agatagtcta tcgtggcgcg
     6781 gtcaccggcg acataggccc ccgccgcgcc cagccccttg gagaaggtcc ccatctcaag
     6841 gaacgctttc cccgcgagac cccgctcctc catcagcccg ccacccgtcg cgccatagac
     6901 ccccgaggcg tgcgcctcgt cgacgatgag gagggcctcg tattttctgc ataatccgac
     6961 gatatcttcc agcggcgcga tgtcgccgtc catgctgaag atcgtgtcgg tgaccagcag
     7021 tttccgcgcg gcggtcgacc gcttcagaag ccgttccaga tcggcgatat cgttgtggcg
     7081 atagcggtgt atcttcgccc gcgaaagtat cgcgccgtcg acgatggagg cgtggtcgag
     7141 tttgtcgagg aagatatcgg tgtcgcggtc ggcaagcgcc gcgatgatgc ccacattcgc
     7201 cgcgtagccc gatccgaaat agagcgccgc ctcgcgcttc ttccacgcgg cggtcctctc
     7261 ttccagttcc ttcacccacg gcccggtgcc ggtgatgagc cgcgacgccg acgcgccgac
     7321 cggccacttt tcaagcgccg cgatcgccgc ctttttgagc gccggatggc tgtcgagacc
     7381 gaggtaggaa ttcgatgaga aattgacgac ccgccgtccc cggaactcgc cgcgcagggc
     7441 cgagccgtcg gggacgaaat ccttcagttc ccgtttgagt cccgccgccg ccagttcgtc
     7501 gagctctttt tgtatgaagt cgttcatgcg ccgcaggccg cgaggatcgc gggccagtcg
     7561 aaccgttcct gtagggcgtc ggccgcttcg ggggtgagcc aaccgggccg ttcggtgtcg
     7621 agtccgagta tgcgcggtag caccccgaga agcggtgcgt tgcagaggcg cggcaggtgg
     7681 aggatgttcc ggtcggcggt ctcttcgccc ggcgcgtagc ccgacacgac gaccccggcg
//
LOCUS       DAOQUE010000072         7686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_10506_length_7686_cov_3.823221, whole genome shotgun sequence.
ACCESSION   DAOQUE010000072 DAOQUE010000000
VERSION     DAOQUE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10506_length_7686_cov_3.823221"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1297)
                     /locus_tag="PKH10_03405"
     CDS             complement(158..1297)
                     /locus_tag="PKH10_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNT27205.1"
                     /translation="MPVDAALITLRRELHEHPELAFQEIATRDILERFVTATVAGDRR
                     FRIIRCLETALIVEYRGADTREPFLLFRADMDALPLTEDPTHPVVSKRPGLMHACGHD
                     VHMTVLAALVARVVAHGPDRNVLFVFQPGEEGAGGAKRMLDAGLFDGYAVRAAFALHV
                     TDEHLLGEAATRTGVLFAMPREVDIAFTGTAGHAAFPQKAHDALFAATRFVQQVPASL
                     AKTLDPMRPFLFHVGRIAGGTARNIVADSCVLNATMRALDPVTMERGAAVVEETARAA
                     AAQAGCAYRIEALGEFLPVVCDGALVERFREICARTGTRFVESDTKLVGEDFGFFCAR
                     WPSLLFWLGTRREGEPPRALHTAALYPPDEAIARGAALFEALLNV"
     gene            complement(1377..2129)
                     /locus_tag="PKH10_03410"
     CDS             complement(1377..2129)
                     /locus_tag="PKH10_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27206.1"
                     /translation="MKVQGWVFVVSNPSAPELSRISFSVKQPATRVEELNRTKDGLPF
                     VLEYEVIVDHPQRYKELACRELTAAGKHEWKEWFRCSPVEAAMVIRKVIGEHGIVAES
                     LKRLEKTGRDEVAFPDRETMESVRPETVAPEEASRKDEDRARSEREKLHEELEKARQE
                     LAAQEKRPDEGPAVKELNIEVSSDIPERGFPWDYVALLVADTGCIYIAFSATGLFTAV
                     LAALLAVGLSALLAYLIWSYHEETKKRDATKK"
     gene            complement(2233..3456)
                     /locus_tag="PKH10_03415"
     CDS             complement(2233..3456)
                     /locus_tag="PKH10_03415"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNT27207.1"
                     /translation="MTSSKGTVAILCAGGPAPGINTVVGALSKSFLNDGWRVLGINYG
                     FKTIFTDKPDTVEMDHLLADRIFTVGGSFLKMSRHKPKDAEFKPDFFVRNDIRLLVTV
                     GGDDTASTANRLTKWLADQRVQVRNIHVPKTIDNDLPLPGRLPTFGYRSAQNEGTRIA
                     MTVYEDARTSGNWFVVSMMGREAGHLAWGIGNACHYPVIIIPEMFTKVEITFDRMIRM
                     MVGAIVKRKLLGMNYGAIAVSEGVFHFMTDDEIRSCGVQFTFDDHGHPELGNVSKAHI
                     FNALLGAKLKELGISVKSRPVEIGYEIRCNTPIAFDLEYCTALGVGVKRLYEQGATGC
                     IVVVGEDGTVKPLYLKDIAGSDGKVRTRMVDITTDDFRMLFDAMHSLKPADHAAAKKY
                     LPDPENYDLAKILGW"
     gene            3661..4857
                     /locus_tag="PKH10_03420"
     CDS             3661..4857
                     /locus_tag="PKH10_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT27208.1"
                     /translation="MRTKAIFGKCDAYDRTLIAKREGYYPYFKTITSEQDTEVYLANG
                     QKVLMLGSNSYMGLTNHPEVKAAAKKAIEKYGSGCAGSPFLNGTLDIHEELAAALAAF
                     VGKEAVLLYSTGFGVNQGVISTLTGRHDLIFMDALNHASIIEGSRLSFATVKRYEHND
                     MADLEKLLASTPREKGKFIVADGVFSMDGDILKLPALVELAEKYDAVVMVDDAHALGV
                     LGPKGDGTAAHFGLTDKVDLIMGTFSKSLASIGGFIAGDAQTIEFLKHHSRALMFSAS
                     MPPASAASALAALRVMEREPWRIEKLWRNTTMLREGLQKLGFNTGLSETPVIPFFVGS
                     DMNAFFMRKRLEEEGVFVNPVISPAVAKDQALIRLSLMSNHTEEQIAFALEKLALCGR
                     EIGLIP"
     gene            4854..5993
                     /locus_tag="PKH10_03425"
     CDS             4854..5993
                     /locus_tag="PKH10_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27209.1"
                     /translation="MIEVRAVTDRRSLGHFLDLPYRLYRHDPNWVPPLRFERKMFFDR
                     KKNPFFEHGEADYFVAYRDGVPVGRITAHIDHNYDAYHKTRHGFFGFFECENDPEAAR
                     VLIETAENWVKARRYERIIGPMNFTTNHDSPGFSTFGADQPPLLMNPHTMPYYPDLFR
                     TAGYVDEQMLNSWILEEPKALPQEFRNRDEKLCAKYGDRLKVRHIDLKRLRQDVDIIL
                     KIFNEAWCDNWGYVPMTDREIAAMADELKLIVDLPLTYLVFLDDEAVAFMVAMPDINR
                     ILIKNRDGSIFSRHFLKLILNRKKHIDRFRVMLLGTLKKYRLLGFDALMYHRLFHDGY
                     AAGYLTVEMSWILDTNKPMNNAAAHMGAHLYQQYLILAKPLGPTT"
     gene            5990..6979
                     /locus_tag="PKH10_03430"
     CDS             5990..6979
                     /locus_tag="PKH10_03430"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT27210.1"
                     /translation="MKEVLVTGATGFVGGHLTELLLAKGIRPRLLTRRRNDDVSRFEK
                     EGASIYVGEQGDTALLREATDGCDTVIHVAGATKRLIEQEYLDGNLRFTEALLAPLTG
                     GQRFILVSSQAAAGPSTGDRPVVESDPPRPLTWYGKSKLAAEEAVRKWGAEHGDKYLI
                     LRPCSVFGPNERDIYTYFQFMDKGILLLLGTGGKKISIIHVADLCDAIVAAAESTVTG
                     RTFFVANDVPVSWRELGETIRVAMGKKNVLRIAIPEWVAAPIGHAAGLAGRLMGRALP
                     VNGQKVIEMKQDAWICSNGAIKEALDWRPRLPLAEAVAQTVAWYRANGWLKQL"
     gene            6989..7561
                     /locus_tag="PKH10_03435"
     CDS             6989..7561
                     /locus_tag="PKH10_03435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNT27211.1"
                     /translation="MSETFIHPSSYIDDAVEIGVGTKIWFFCHVLTGSRIGRDCSFGQ
                     NCMIGPRALIGNGVRVQNNVSIYEGVVIEDDVFLGPSMVFTNVINPRSFIPRKDEYRK
                     TLLKKGCSVGANATIVCGVTIGEYAFVGAGAVVTKDVPAYALVYGSPAKQHGWVGRSG
                     NKIVFGADGTATDVSDGARYELKNGITRPL"
BASE COUNT         1534 a         2483 c         2258 g         1411 t
ORIGIN      
        1 ccttggtgaa ccagtatttg tcccactgcg gagcgacgaa ttgccacgcg aaccagatga
       61 tgatcgcgcc gataaggatc tttacggcat cggacatgtg catggatagt accctccgtt
      121 cggtgtcggg gcgttgtaac aaaaagccgt ggcaatgtca aacattcagg agcgcctcga
      181 aaagcgccgc gccccgcgct atcgcctcat cgggcggata aagggcggcg gtgtggagcg
      241 cacggggcgg ctcgccttcc cggcgggtac cgagccagaa aaggagcgac ggccaccggg
      301 cgcagaagaa cccgaaatcc tcccccacca gtttggtgtc cgactccacg aaacgggtgc
      361 cggtacgggc gcagatctcc cggaaccgct cgaccagagc cccgtcgcag acgaccggga
      421 ggaactcgcc gagcgcctcg atgcgatagg cgcacccggc ctgcgccgcc gccgcccgcg
      481 ccgtctcctc gacgaccgcc gcgccgcgct ccatcgtcac ggggtcgagc gctcgcatcg
      541 tggcgttaag gacacaggaa tcggcgacga tgttgcgcgc cgtaccgccc gcgatgcggc
      601 cgacatggaa taggaagggg cgcatcggat cgagcgtctt ggcgagcgat gcggggacct
      661 gctgaacgaa ccgggtcgcg gcgaacagcg catcgtgcgc cttttgcggg aatgcggcgt
      721 gccccgccgt gccggtgaag gctatgtcga cctcgcgcgg catcgcgaac aggacgcccg
      781 tccgcgtcgc tgcctcgccg agcaggtgtt catcggtgac atggagcgcg aaggccgccc
      841 ggacggcgta tccgtcgaag agtcccgcat cgagcatccg tttcgcgccg cccgccccct
      901 cttcgcccgg ctgaaagacg aacaaaacat tgcgatcggg cccgtgtgcg acaacccgtg
      961 cgacgagcgc ggcgagtacg gtcatgtgga catcgtggcc gcaggcgtgc atgagacccg
     1021 gtcgtttcga gacgaccgga tgcgtcgggt cctcggtgag cggcagggca tccatatcgg
     1081 cacggaacag gaggaacggc tcccgcgtat cggcgccccg gtactcgacg atcagcgccg
     1141 tctcgagaca ccggatgatg cggaaccggc ggtcgcccgc gacggtcgca gtgacgaacc
     1201 gctcaagtat atcgcgcgtg gcgatctcct gaaaagcaag ttccgggtgc tcgtgcaatt
     1261 cccttcggag ggtgatgagc gcggcatcta caggcatggc tttcctccat cggtcatcgt
     1321 gaccgttggc cctaccatac cctgaaagga cgcgcgacac aagaaagtcg gcgcgatcat
     1381 ttcttcgtcg cgtccctttt cttcgtttct tcgtggtagg accagatgag atacgccaac
     1441 aacgcgctga gacccacggc gagcagggcg gcgagcacgg cggtgaaaag cccggtggcc
     1501 gagaacgcga tgtatatgca gccggtgtcg gcgacgagga gcgcgacata atcccacgga
     1561 aaacctcttt cggggatatc agacgacact tcgatgttca actcttttac cgccggtccc
     1621 tcgtcggggc gtttctcctg cgccgccagt tcctgtcgcg ccttttccaa ttcctcgtgc
     1681 agtttttccc gttcgctccg cgcgcggtcc tcgtccttcc tgctcgcctc ctccggcgcc
     1741 accgtttccg gtcggacact ctccatggtc tcgcggtcgg ggaaggccac ctcatcgcgc
     1801 cccgtcttct cgagccgttt gagcgattcg gccacgatcc cgtgctcgcc gatgacctta
     1861 cggatgacca tcgccgcctc gaccggcgaa caccggaacc actccttcca ctcgtgcttt
     1921 cccgccgccg tcagttcccg gcacgccagt tccttgtatc gttggggatg atcgacgatg
     1981 acctcgtact cgaggacgaa cggcaggcca tccttggtcc tgttcagttc ctccacccgg
     2041 gtcgccggct gtttgaccga gaaacttatc ctgctcagtt caggcgcgga aggattggag
     2101 acgacgaaca cccagccctg gaccttcatg cgtcacctcc actcctcgta caatgaactt
     2161 ccccgagatc ggctaaaggt aatgataccc gatgcgggga gtgaagtcaa tcggtatctc
     2221 gaccggaagg attcaccagc cgagtatctt cgcaagatcg taattctcgg gatcgggcag
     2281 gtattttttc gctgccgcgt ggtcagcggg cttgagagaa tgcatcgcgt cgaagagcat
     2341 cctgaagtcg tcggtcgtta tatccaccat acgggtccgc accttgccgt cggaccccgc
     2401 gatgtccttg aggtacagcg gcttcacggt gccgtcctcc cccaccacaa cgatacagcc
     2461 ggtggccccc tgttcgtaga gacgcttcac gccgacgccc agcgcggtgc aatactcgag
     2521 atcgaacgct atcggcgtgt tgcaccgtat ctcgtacccg atctcgaccg gtcggctttt
     2581 gacactgatg ccgagctcct tgagtttcgc gccgagaagc gcgttgaata tgtgggcctt
     2641 gctgacattg cccagttcgg ggtgaccgtg atcatcgaag gtgaactgca caccgcagga
     2701 ccgtatctcg tcgtcggtca tgaagtggaa caccccctcc gagaccgcga tcgcgccata
     2761 gttcatgccg agcagtttcc gcttgactat cgcaccgacc atcatccgga tcatccggtc
     2821 gaaggttatc tcgaccttcg tgaacatctc ggggatgatg atgaccgggt agtggcacgc
     2881 attcccgata ccccatgcga gatgccccgc ctcacgcccc atcatcgaga cgacgaacca
     2941 gttgccgctc gtgcgggcgt cctcgtagac cgtcatcgcg atgcgggtcc cttcgttctg
     3001 ggcgctccgg tacccgaagg tcggcaggcg gccgggaagc ggcagatcgt tgtctatcgt
     3061 cttggggaca tgtatgttac gcacctgcac cctctggtcg gcgagccatt tggtgagccg
     3121 gttcgccgtg gacgcggtgt catcgccgcc gaccgttacg agcaatcgga tatcgttgcg
     3181 cacgaagaaa tcgggcttga actcggcgtc cttcggtttg tggcggctca tcttgaggaa
     3241 ggagcctccg acggtgaata tccggtcggc gagcagatga tccatctcga ccgtatcggg
     3301 cttgtcggtg aagatggtct tgaaaccata gttgatgccg agcacgcgcc agccatcgtt
     3361 caagaacgac ttggatagcg caccgaccac cgtgttgatg ccgggcgccg gaccgccggc
     3421 gcacagaatg gcgaccgttc ctttcgatga tgtcatgggt tcctccgttc gtaaaagagt
     3481 tccgttcaac gcgcccatgg tagcgtggag aaaagaaaaa agcaaatacc cgcggctctc
     3541 gccccgcgcg aagacccgct aaaaaattga ttcgtcgcgc cttgtgtgat aaggttccgc
     3601 gtccgctccg gagttccgcc gcgtcggcgg ggtccgcgca ccggcaaagg agaaatgccc
     3661 gtgagaacca aggccatctt cggcaaatgc gatgcctacg accgcaccct catcgccaaa
     3721 cgcgagggct actaccccta cttcaagacc atcacatccg aacaggacac cgaggtctat
     3781 ctcgccaacg ggcagaaggt gctcatgctc ggttcgaact cctacatggg cctcaccaac
     3841 catcccgagg tcaaggcggc ggccaagaag gcgatagaga aatacggttc gggctgcgca
     3901 gggtcgccgt tcctcaacgg cacgctcgac atccacgagg aactggcggc cgcactggcc
     3961 gccttcgtcg gcaaagaggc ggtgctcctc tactcgaccg gcttcggcgt caatcagggg
     4021 gtcatctcca cgctcaccgg acggcatgat ctcatcttca tggacgcgct caaccatgcc
     4081 tcgatcatcg aggggtcgcg tctgtcgttc gcgacggtca aacgctatga acacaacgac
     4141 atggccgacc tcgaaaaact gctcgcatcg acgccccgag aaaagggcaa attcatagtc
     4201 gccgacggcg tcttctcgat ggacggcgac atactgaaac ttccggcctt ggtcgaactc
     4261 gccgaaaagt acgacgcggt cgtgatggtc gatgacgccc acgcgctcgg cgtgctcggg
     4321 ccgaaaggcg acggcaccgc cgcccatttc gggctgaccg acaaggtcga cctcatcatg
     4381 gggaccttca gcaaatcgct tgcatccata ggcgggttca tcgccggcga cgcccagacg
     4441 atagagttcc tcaagcatca cagccgcgca ctgatgttca gcgcctcgat gccccccgcc
     4501 tcggccgcct cggcgctcgc ggcgctccgc gtgatggaac gggagccgtg gcgcatcgag
     4561 aaactgtggc gcaacacgac catgttgcgc gaaggactcc agaaactcgg gttcaacacc
     4621 ggcctctccg agacccccgt catccccttt ttcgtcggca gcgacatgaa tgcctttttc
     4681 atgcgcaagc ggctcgaaga ggagggcgta ttcgtcaatc cggtcatcag ccccgccgtc
     4741 gcgaaggatc aggcgctcat ccggctcagc ctcatgtcga accacaccga ggaacagatc
     4801 gctttcgccc ttgaaaaact ggcgctgtgc ggtcgcgaga taggtctgat cccatgatcg
     4861 aggtacgcgc cgtcaccgac cgccgctcac tcggccactt cctcgatctc ccttaccggc
     4921 tctaccgtca cgacccgaac tgggtgccgc cgctccgctt cgagaggaaa atgttcttcg
     4981 accggaagaa gaaccccttc ttcgaacacg gcgaggccga ctattttgtc gcctaccgcg
     5041 acggcgtccc ggtcggacgc atcaccgcgc atatcgatca caactacgac gcctaccaca
     5101 agacccgcca cggttttttc ggtttctttg aatgcgagaa cgatcccgag gcggcccgcg
     5161 tgctcatcga gacggccgag aattgggtca aggcccgcag atatgaacgg atcatcggcc
     5221 cgatgaattt caccaccaac cacgattccc ccgggttctc caccttcggc gccgaccagc
     5281 cgccgctcct gatgaacccc cacaccatgc cctactaccc cgacctgttc cgcaccgccg
     5341 gatatgtcga cgaacaaatg ctcaacagtt ggatactgga ggaaccgaag gccttgccgc
     5401 aagagttccg gaaccgcgac gagaaactgt gtgcgaaata cggcgaccgt ctgaaggtgc
     5461 gccacatcga tctcaagcgc ctgcggcagg atgttgatat catcctgaag atattcaacg
     5521 aagcgtggtg cgacaactgg ggctatgtgc cgatgaccga ccgcgagatc gcggcgatgg
     5581 ccgatgaact caaactcatc gtggacctgc cgctcaccta tctcgtcttc ctcgacgacg
     5641 aagcggtcgc cttcatggtc gcgatgcccg acatcaaccg gatactcatc aagaaccgcg
     5701 acggctcgat cttcagccgc catttcctca aactcatcct caaccggaag aagcacatcg
     5761 accgtttccg ggtgatgctg ctgggcaccc tgaagaagta ccgtctgctc ggtttcgacg
     5821 cgctcatgta ccaccgcctg ttccacgatg gctacgccgc cggatatctt accgtcgaga
     5881 tgtcgtggat actcgacacc aacaagccga tgaacaatgc ggcggcccat atgggtgccc
     5941 acctctacca acagtacctc atcctggcca agccactcgg cccgaccaca tgaaggaggt
     6001 gctcgtcacc ggcgccaccg gctttgtcgg cggccatctg accgaactgc tacttgcgaa
     6061 aggcatccgg ccccgcctgc tcaccagacg acgtaatgac gatgtttcgc gattcgagaa
     6121 ggagggcgcg agcatatatg tcggcgagca gggcgatacc gcgctccttc gcgaggcgac
     6181 cgacggctgc gacacggtca tccatgtcgc cggcgccacc aaaagattga tcgaacagga
     6241 gtatctcgac ggcaaccttc gtttcaccga ggcgctcctc gcgccgctga ccggcggaca
     6301 acgcttcatc ctcgtcagtt cgcaggcggc ggccggccct tcgaccggag accgaccggt
     6361 ggtcgagagc gatccgcccc gccccctcac ctggtacggc aagagcaaac tcgccgccga
     6421 ggaggcggtc aggaaatggg gagcggaaca cggcgacaag tacctgatac tgcgtccctg
     6481 ttcggtcttc ggtcccaacg agcgcgacat ctacacctac ttccagttca tggacaaagg
     6541 catactcctc ctgctgggga ccggcgggaa gaaaatctcg atcatccatg tcgccgatct
     6601 ctgcgacgcc atcgtcgcgg cggctgaaag caccgtgacg ggacgcacct tcttcgtcgc
     6661 caacgacgta ccggtctcgt ggcgcgaact gggcgagacc atccgcgtcg cgatggggaa
     6721 aaaaaatgtc ctgcgcatcg cgataccgga atgggtcgcc gcccccatcg gccacgcggc
     6781 ggggctcgcg gggcggctca tgggacgcgc tctgcccgtc aacggccaga aggtcatcga
     6841 gatgaaacag gatgcgtgga tatgcagtaa cggcgcgatc aaggaggcgc tcgactggcg
     6901 accgcgcctg ccgcttgccg aagcggtcgc gcagaccgtc gcgtggtacc gtgcgaacgg
     6961 ctggctgaaa caactctagg agaagaccat gagcgagaca ttcatccatc cgagcagtta
     7021 tatagacgat gctgtggaga tcggcgtagg caccaagata tggttcttct gccatgtgct
     7081 gaccggttcg cgcatcggcc gcgactgctc gttcggccag aactgtatga tcgggccgcg
     7141 tgccctcatc ggcaacggcg tccgggtaca gaacaacgtc agcatctatg agggggtcgt
     7201 catcgaggac gatgtgttcc tcggcccttc gatggtattc accaacgtga taaacccgcg
     7261 cagtttcata ccgcgcaagg acgaataccg gaagacactg ctcaaaaaag gctgctcggt
     7321 cggcgcgaac gccaccatcg tctgcggggt caccatcggc gagtacgcct tcgtcggcgc
     7381 aggcgcggtg gtcacgaagg atgtccccgc ctacgcgctt gtctacggct cgccggcgaa
     7441 acagcacggc tgggtcggtc ggtcggggaa caagatagtg ttcggggccg acggcaccgc
     7501 gaccgatgtt tctgacggcg cccgctatga actgaagaac ggcatcacga ggccgctgtg
     7561 aaaaagatag aactcatcga tctcgcggct cagtaccgcg cctacaaaga cgaattcgac
     7621 gccgccatcg gtgcggtctg ctcccgcgcc tcgttcatca tgggatccga ggtggccgaa
     7681 ctcgaa
//
LOCUS       DAOQUE010000073         7630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_10608_length_7630_cov_4.513399, whole genome shotgun sequence.
ACCESSION   DAOQUE010000073 DAOQUE010000000
VERSION     DAOQUE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10608_length_7630_cov_4.513399"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PKH10_03440"
     CDS             <1..865
                     /locus_tag="PKH10_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27212.1"
                     /translation="GTAYGKADIAVAKFDADGAPIWIKQFGSAEEDRAFSAVAIGTTL
                     YVGGSTTGKFLPGTVPQQEDGFIVEIDPDGNIVRSTLIGSEGVDRVTALFPGAADTLY
                     AAGTTTGAIHGNGSFGNTDGFLANYDLSLERRWTRQVGTSEADMMITGVAGPENILLA
                     GMTFGSFDDEINAGNYDALLLAFDLNGDRLFSRQHGNDGPDAFHGIAVAPDGSIVATG
                     SSFGPVFSQPATGGYEIITVRYDRSGRLLDTLQYGSAGDDGAYAAVANGAGATLTGVV
                     DMNYFLMDIKW"
     gene            948..2843
                     /locus_tag="PKH10_03445"
     CDS             948..2843
                     /locus_tag="PKH10_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27213.1"
                     /translation="MKRSVVPLMTALFCAPLAAALFGGGPYEVFDQGGTDHFLFGNTF
                     LADQRTIDGLSGDRQGDTCVEKSASALTIDMFFPDAIVDKAYLVWTGAVDPAKLEEPT
                     DHTVHLSFVREDGYGYEADIAAGAAPRHLGDTEDPFTFESVRFTTDVRVGCSETSPGT
                     VTAAELAYFTYRADATDFFERILRDNALTEAPMMPGQAVAGEYAVSGLDCTDDEIYRC
                     STLMVSNWAMLIVYRSEEIVPPKNIYLYPGFAFLKEESVDTRLEGLVLPADTPVRMTM
                     LSAEGDPALMTAREGSEMVAVRGNDGAVVPITGDCDPEDEAVNEVWDSRRSFFALSES
                     GAYCTAYDDEPYTFGLDIDRWELDPTIDTRLAGIFSEDGDTIDLRFRFAADEVLTNLV
                     VLAVERPVSDFDVPGADELRDCPCVPEGTRDIFCEGEPLYILLTVENWGETFAESVYV
                     RAEYDRAVFDYLPGSTEIATRFDRAGNGTNWQVFLDGPAGGFPLAEPNLIANTLPSSL
                     SAPDEALSYLIRFQLVPKTGLPKNTVGKVSAVISDTNGSYRTNHHLPLRLYPGACLQT
                     CTEKELRERCGGMTADNPQIYDPQEKEGDPPDADETPDLVPDDIIDPRATHPMSAQEP
                     GCALILF"
     gene            complement(2858..3838)
                     /locus_tag="PKH10_03450"
     CDS             complement(2858..3838)
                     /locus_tag="PKH10_03450"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNT27214.1"
                     /translation="MRYREGLNGGFFSAIQYAVADRGGLVGEKAEGLTAFEHGAPVTA
                     RTMFDLASLTKALFTAPACYLLFDTGLVRPDDTLDRFFPGQRPVPLLRLLNHTSGYPA
                     YLEFFRDTAAKRYDDRRAAVLARIAALRDIEPPIYSDLNFILLGFVLEKVTGEPLDAV
                     WARLLDDLNFPQEDIVFAKRPLLKQHTVATMFSKERGRLCHGEVEDENCWYLGSVTGH
                     AGLFGSAGSVASYLMRLLGRPWFRSALDRLGGAGFDRPQGLDSHYGRTAHARTVGHLG
                     FTGTAFLLDPEKEKAAVVLTNRTHPDPDKENWRDRIKAVRRELFEVLLPA"
     gene            complement(3843..4034)
                     /locus_tag="PKH10_03455"
     CDS             complement(3843..4034)
                     /locus_tag="PKH10_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007395922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNT27215.1"
                     /translation="MTLRKSRNKVIFGCLGGVSEYFGWDPTIVRVVYVLLSILSAAFP
                     GLLVYLILFILMPSADSEV"
     gene            4184..4792
                     /locus_tag="PKH10_03460"
     CDS             4184..4792
                     /locus_tag="PKH10_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HNT27216.1"
                     /translation="MKKWTVLMLCVAVIFSVSCASQSHKVTRTASDEAIDLSGRWNDT
                     DSRLVAEEMIRDVLSRAWIDDFNGANEGKKPVVIVGRIRNKSSEHIATDVFIKDLERE
                     LINGGRIKFVASKAERGELRSEKEDQQSNATEDSAKKLASETGADFMLQGTISSVTDA
                     VEGQKVVFYQVDLELVNIESNEKVWIGSKKIKKVVEQAKSKW"
     gene            4837..6246
                     /locus_tag="PKH10_03465"
     CDS             4837..6246
                     /locus_tag="PKH10_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27217.1"
                     /translation="MFQRGSIISIMLAAALFLVACGTSKAQYAGVDDKLRRGDYVSVV
                     KQISDLKEKEYGEKNRVLFYLDLGMLMHYAGQYQQSNELLTQAENAIEELYTVSISKE
                     AGAAIVNDNTREYSGEDYEDVYVNIFKALNYLALNDAEGAAVEAKKVHIKINKLKDKY
                     PIEDEEVKKQELPVFKESALAYYMSMLLYRMEKAWDDAEIDRKKLMEAMSGQKGVYIK
                     LSNPEEAITLEDEALATAIPEGKGVVSVVSFNGLGPEKMARILNVNTLGGGINISSRL
                     DDKKDGAKADVRALFQYPQMRIPIPLMPPGYNFTVEFPEMVSKRPMAHRIEVVVDGAQ
                     KMDLFPIENISRVAEVTFAQKIASTYVKTLIRAGVKFAAAVVASEAANKAGGGLVGAI
                     TRFGTSTIASATEKADLRLSYFFPGEAFAGELALDPGEHQVTVTYYYGGGVIHQEKKT
                     VTVKEGDLQVLETFYLGSR"
     gene            6248..7237
                     /locus_tag="PKH10_03470"
     CDS             6248..7237
                     /locus_tag="PKH10_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27218.1"
                     /translation="MKKLLFSLVLLGSFAAFAGDDCMALMQDPKGTYPGGAFLSAVGD
                     GDSLSGAQSRAMSGLSAIFEVTIKNELSVRESSETKGGKEDYRSSISDVSKIQTNQKL
                     MNVQFSENCTDKKTGKVYVIAYLDRAKTAPLYVEKIKDLDAQVEKALGRAKAEPEPIK
                     AFVFLRQAVEKARENEAQKNTLRIIAAPALAALKDSRPLSAVADLLYEKVRDLPVSIT
                     VQNDKDDRLKSAFDAAFKKMGFLETGAAEGKIRLNAVFTVEDAGLKNKYYNARWTLNI
                     TLADQTGKTILTRSENQRATAVSAADAIHRSFKDAEKAIYPILNKAIEALTAK"
     gene            7300..>7630
                     /locus_tag="PKH10_03475"
     CDS             7300..>7630
                     /locus_tag="PKH10_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27219.1"
                     /translation="MLFLLCAILSSSAVALILKYAEFRAYRRLPVVAANYLAAAILSV
                     LLAGGALIPDGVPDDLVATFLAEASAVFSGSGVFSLGGSIGWAVLWGVPAGLLYFLGL
                     ISIQQSIR"
BASE COUNT         1545 a         2350 c         2420 g         1315 t
ORIGIN      
        1 gggaacggcc tacggcaagg ccgacatcgc ggtcgccaag ttcgacgccg acggcgcacc
       61 gatatggata aaacagttcg gctccgccga ggaggaccgt gctttttcag ccgttgctat
      121 cggcacaacg ctctatgtcg gcggttcgac caccgggaaa ttcctgccgg gcaccgtgcc
      181 gcaacaggag gatggcttca tcgtcgagat cgaccccgac ggtaatatcg tccgttcgac
      241 gcttatcggc agtgaggggg tcgaccgggt cacggcgctg ttccccggcg cggccgacac
      301 cctctacgcg gccgggacca cgaccggcgc gatccacggc aacggttcgt tcggcaatac
      361 cgacggtttt ctcgcgaatt acgatctgtc cttggaacgg cgctggaccc ggcaggtcgg
      421 cacctccgaa gccgacatga tgatcacggg ggtcgccggg ccggagaaca tactgctcgc
      481 cgggatgacc ttcggatcgt tcgacgacga gataaacgcg gggaactacg acgcgctcct
      541 gctcgcgttc gacctgaacg gcgaccgcct gttcagccgc cagcacggca acgacggtcc
      601 cgacgcgttc cacggcatcg ccgtcgcccc cgacggatcg atcgtcgcca ccggctcatc
      661 gttcggcccg gtattttcgc aaccggcgac gggcgggtat gagatcatta cggtccggta
      721 cgaccgctcc ggccgcctgc tcgacaccct gcagtacggc tccgccgggg atgacggcgc
      781 gtacgccgcg gtcgcgaacg gcgccggcgc gacgcttacc ggggtcgttg acatgaatta
      841 tttccttatg gatatcaaat ggtaaccgtt tttttcgccc ggaccggaat ccttttgccc
      901 gcgccgggtc tcttatgggg tgagaaacgg atggagggaa aaggtcggtg aaacggtcgg
      961 tcgttccgct catgacggcg cttttctgcg ccccgctcgc ggccgccctg ttcggcggcg
     1021 gaccgtacga ggtgttcgat caggggggga ccgatcattt cctgttcggc aacaccttcc
     1081 tcgcggacca gcggaccatc gacggcctgt cgggagaccg gcagggcgac acctgtgtcg
     1141 aaaaaagcgc ttctgctctg accatagata tgttttttcc ggacgccatc gttgacaagg
     1201 cctatctcgt ctggaccggc gcggtcgatc cggcgaaact cgaagaaccg accgaccata
     1261 cggtccatct ttcgttcgtc cgggaggacg gatacggcta tgaggccgac atcgccgcgg
     1321 gcgccgcgcc gcgccatctc ggcgacacgg aggatccctt caccttcgag agcgtccgct
     1381 tcacgaccga tgtccgcgtc ggttgttccg agacctcgcc gggaacggtg accgccgccg
     1441 aactcgccta tttcacctac cgggccgatg cgaccgactt tttcgaacgg atactgcgcg
     1501 acaatgcgct gaccgaggcg ccgatgatgc cgggtcaggc ggtcgcgggc gaatatgccg
     1561 tgagcggact cgactgcacc gacgacgaga tatatcgctg ttcgacgctg atggtgtcga
     1621 actgggcgat gcttatcgtg taccgctctg aagaaattgt ccctcctaaa aatatctacc
     1681 tctatcccgg gttcgctttc ctgaaagaag agagcgtcga tacccgcctc gaagggctcg
     1741 ttcttccggc cgatacgccg gtgcggatga cgatgctctc cgcggagggc gatccggcgc
     1801 tcatgacggc gcgcgaaggg tccgagatgg tggcggtgcg ggggaacgac ggggccgtcg
     1861 tcccgataac gggcgactgc gacccggagg acgaggcggt caacgaggta tgggattcgc
     1921 gccgatcctt ctttgcgctg tccgagagcg gcgcgtactg taccgcatac gatgacgagc
     1981 cctacacctt cggcctcgac atcgaccgct gggagctcga cccgacgatc gatacgcggt
     2041 tggcggggat attttccgaa gatggcgaca ccatcgatct ccggttccgt ttcgccgccg
     2101 acgaggtgct gaccaacctt gtcgtgctcg cggtggaacg gcccgtgtcc gatttcgatg
     2161 tccccggcgc ggatgaactc cgcgactgtc cctgcgtgcc ggaagggaca cgcgacatct
     2221 tttgcgaagg agagccgctt tatatcctgc tgaccgtcga gaactgggga gagaccttcg
     2281 ccgagtcggt gtatgtccgg gcggagtacg accgggcggt ctttgactat cttcccggct
     2341 ccaccgagat cgcgacgcga ttcgaccggg cgggtaacgg cacgaactgg caggtgttcc
     2401 tcgacggccc ggcgggggga ttcccgctgg ccgaaccgaa cctcatcgcc aatacgctcc
     2461 ccagttccct ttcggcgccg gacgaggcgc tttcctacct gatccgcttt caactggtgc
     2521 cgaagaccgg tctcccgaag aacacggtcg ggaaagtaag cgccgtcatc agcgacacga
     2581 atgggtctta ccggaccaat catcacctcc cgttgagatt gtatccgggg gcgtgtctcc
     2641 agacctgcac cgaaaaagaa ctgcgggaac ggtgcggcgg catgaccgcc gacaacccgc
     2701 agatatacga tccacaggag aaggaaggag acccgccgga cgccgacgag accccggacc
     2761 ttgtccccga cgacatcatc gatccccggg cgacccatcc catgagcgcg caggaaccgg
     2821 ggtgtgcgct gatcctgttc tgatccgccc ggcgccttca ggccggcaga agcacctcga
     2881 acagttcgcg ccgcaccgcc tttatccggt cgcgccagtt ctccttgtcc gggtcgggat
     2941 gagtgcggtt ggtgaggacg accgccgcct tttccttttc cgggtcgagc aggaacgcgg
     3001 tgccggtgaa gccgagatga ccgaccgtcc gggcgtgggc ggtgcggccg tagtgcgaat
     3061 cgagcccctg cggccggtcg aatccggcgc cgcccagccg gtcgagggcg gagcggaacc
     3121 acggccgccc gagcagtcgc atgagatagg aggccacgct tcccgccgat ccgaaaagcc
     3181 ccgcgtgacc ggttaccgag ccgagatacc aacagttctc atcctcgacc tcgccgtgac
     3241 agagccgccc gcgctccttc gagaacatcg tcgcgacggt atgctgtttc agaagcggtc
     3301 gtttcgcgaa cacgatatct tcctgcggga agttcaggtc gtcgaggagc cgggcccaca
     3361 ccgcgtcgag cggttcgccc gtcacctttt cgagcacgaa cccgagcagg atgaagttga
     3421 ggtcgctgta gatcggcggc tcgatgtcac ggagcgccgc gatgcgcgcg agcaccgccg
     3481 cgcggcggtc gtcgtaccgt tttgccgcgg tgtcgcggaa gaattcgagg taagcgggat
     3541 acccggaggt atgattgagg agccgcagga gcggcacggg acgctggccc gggaagaagc
     3601 ggtcgagcgt atcgtcgggc cgaacgaggc ccgtatcgaa caggagatag caggcgggcg
     3661 cggtgaagag cgccttggtg agcgaggcga gatcgaacat cgtccgcgcg gtgaccggcg
     3721 cgccgtgctc gaaggcggtc agcccctcgg ccttctcacc gacgagtccg ccacggtcgg
     3781 cgaccgcgta ctggatcgcc gagaagaacc cgccgttcaa tccctcccga tagcgcacgc
     3841 cgtcagacct cgctgtcggc cgacggcatg aggatgaaca ggatgagata gacgagcagg
     3901 ccggggaagg cggcgctgag tatcgacagg agcacataga cgacccggac gatggtcggg
     3961 tcccagccga aatactccga tacgccgccg agacagccga agatgacctt gttgcgcgat
     4021 ttcctgagcg tcatgggcgc ctcctgttag ggtcgtgacg gcgtatcata gcaaaaaaac
     4081 gaccgccggt caaaaaccgt ttttcttgac aaaagaggcc cgaaaaaaca agatgtacgc
     4141 gttacggcgt tatacaccaa ccctgataca tggaggagac atcatgaaga aatggacggt
     4201 acttatgctt tgcgttgcgg tcatcttttc ggtatcctgc gcgtcccaga gccacaaggt
     4261 cacccgcacc gcgtccgatg aggcgatcga tctgagcggc cggtggaacg acaccgattc
     4321 ccggctggtc gccgaggaga tgatccgcga tgtgctgtcg cgcgcgtgga tagacgactt
     4381 caacggcgcg aacgaaggga aaaagccggt cgtcatcgtc ggccgcatcc gcaacaagag
     4441 ttccgaacac atcgcgaccg atgtcttcat caaggacctc gaacgcgaac tcatcaacgg
     4501 cggtcgcatc aagttcgtcg cgagcaaggc ggaacggggc gaactgcgga gcgaaaagga
     4561 ggaccagcag tcgaacgcga ccgaggactc ggccaagaaa ctggccagcg agacgggcgc
     4621 cgacttcatg ctgcagggga ccatcagttc ggtgaccgac gcggtcgaag ggcagaaggt
     4681 ggtgttctat caggtcgatc tcgaactcgt caacatcgag tcgaacgaga aagtgtggat
     4741 cggcagcaag aagataaaga aggtcgtcga acaggcgaag agtaagtggt agcaccgtcg
     4801 gtctcaccgt agttctcaca aacaagggag gttctcatgt tccagcgcgg gagtatcatc
     4861 tccatcatgc tcgcggcggc gcttttcctc gtggcctgcg ggacctcgaa ggcgcagtac
     4921 gcgggggtcg acgacaaact gcggagaggc gactatgtca gcgtcgtcaa gcagataagc
     4981 gacctgaagg aaaaggaata cggcgaaaag aaccgggtgc tgttctatct cgacctcggg
     5041 atgctcatgc actacgccgg ccagtaccag cagagcaacg aactgctcac gcaggccgag
     5101 aacgccatcg aggaactgta caccgtgagc atctcgaagg aggcgggcgc ggccatcgtc
     5161 aacgacaaca cgcgcgaata ctccggcgag gactacgagg atgtctatgt gaacatcttc
     5221 aaggcgctca actacctcgc gctcaatgac gccgaagggg cggcggtcga ggcgaagaag
     5281 gtccacatca agataaacaa attgaaggac aagtacccca tcgaggacga ggaggtcaaa
     5341 aagcaggaac tcccggtgtt caaggagagc gcgctcgcct actatatgag catgctcctg
     5401 taccgcatgg aaaaggcgtg ggacgacgcc gagatagacc gcaagaaact gatggaggcg
     5461 atgtcgggcc agaagggcgt ctacataaaa ctctccaacc ccgaagaggc catcacgctc
     5521 gaggacgagg cgctcgccac cgcgataccg gaaggcaagg gggtcgtgag cgtcgtcagt
     5581 ttcaacgggc tcggccccga gaagatggcg cggatactca atgtcaacac gctcggcggc
     5641 ggcatcaaca tcagttcgcg gctggacgac aagaaggacg gcgccaaggc cgatgtgcgg
     5701 gcgctgttcc agtatcccca gatgcggata ccgataccgc tgatgccccc cggctacaac
     5761 ttcacggtcg aattccccga gatggtgtcg aagcgcccga tggcacaccg catcgaggtg
     5821 gtcgtggacg gcgcgcagaa gatggacctg ttccccatcg agaacatatc gcgggtcgcc
     5881 gaggtgacct tcgcccagaa gatcgcgtcg acctatgtca agacgctcat ccgcgccggc
     5941 gttaagttcg ccgcggcggt cgtcgcttcc gaggccgcca acaaggcggg gggcgggctg
     6001 gtcggcgcca tcacccggtt cggcacgagc accatcgcaa gtgccaccga gaaggccgac
     6061 ctgcggctct cctacttttt ccccggcgag gccttcgcgg gcgaactcgc actcgatccg
     6121 ggtgaacatc aggtgaccgt cacctactac tacggcggcg gcgtgataca tcaggaaaag
     6181 aagaccgtca cggtcaaaga gggagatctg caggtattgg aaaccttcta tctggggagt
     6241 cggtgacatg aaaaaactgt tgttctctct cgtattgctc ggatcgttcg ccgctttcgc
     6301 cggcgatgac tgtatggcgc tcatgcagga cccgaagggg acctatcccg gaggggcgtt
     6361 cctttcggcg gtgggcgacg gcgattcgct ttccggcgcg cagtcccgcg cgatgtccgg
     6421 actttcggcc atcttcgagg tgaccatcaa gaacgaactt tcggtccgcg agagttccga
     6481 gaccaagggg ggcaaggagg attaccgctc ctcgatatcg gatgtctcga agatacagac
     6541 caatcagaaa ctcatgaatg tccagttcag cgagaactgc accgacaaga agaccggtaa
     6601 ggtctacgtc atcgcctacc tcgatcgggc caagaccgcg ccgctctatg tcgagaagat
     6661 aaaggatctc gacgcgcagg tcgaaaaggc gctcgggcgg gcgaaggcgg agccggagcc
     6721 gatcaaggcg ttcgtgttcc tgcggcaggc ggtcgaaaag gcgcgcgaga acgaggcgca
     6781 gaaaaacacc ttgcgcatca tcgcggcccc cgcgctcgcg gcgctcaagg attcgcggcc
     6841 cctttcggcg gtcgccgacc tgctgtacga gaaggtgcgc gatctgccgg tgtccatcac
     6901 cgtccagaac gacaaggacg accggctcaa gtcggcgttc gacgcggcgt tcaagaagat
     6961 gggtttcctc gagacaggcg ccgccgaagg gaagatacgg ctgaacgccg tcttcacggt
     7021 cgaggacgcc ggactcaaga acaaatacta taacgcccgc tggacgctca acatcacgct
     7081 cgccgaccag accggcaaga cgatcctgac ccgcagcgag aaccagcgcg cgaccgccgt
     7141 cagcgcggcc gacgcgatac accgctcgtt caaggacgcc gagaaggcga tctaccccat
     7201 cctcaacaag gccatcgagg cgctcaccgc aaagtgacgc cgcgcggggc gaccgccccg
     7261 taaagttgcg cttcgcggga ctttgcgcta ccctgcctca tgctgttcct cctctgtgcg
     7321 atactgtcaa gcagcgccgt ggcgctcatc ctcaaatacg ccgaattccg cgcgtaccgt
     7381 cggcttcccg tggtcgccgc gaactacctc gccgccgcta tcctgtcggt cctcctcgcg
     7441 ggcggcgcgc tcatccccga cggcgttccg gacgatcttg tcgccacttt tctcgccgag
     7501 gcgagtgcgg tcttttctgg gtccggcgtc ttttcgttgg ggggttccat cggttgggcg
     7561 gtgctctggg gcgtcccggc ggggctgctc tacttcctcg ggcttatcag catccagcag
     7621 agcatccgcg
//
LOCUS       DAOQUE010000074         7603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_10658_length_7603_cov_3.979200, whole genome shotgun sequence.
ACCESSION   DAOQUE010000074 DAOQUE010000000
VERSION     DAOQUE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10658_length_7603_cov_3.979200"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKH10_03480"
     CDS             complement(<1..330)
                     /locus_tag="PKH10_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27220.1"
                     /translation="MKRFVFGFCVLALFAACESGTTYKKFPSDNDQLAGGDELLNDDG
                     QLINDDGQLINDDGQLINDDGQIINDDGQVINDDGQVVTDDGEWPDGEWPDGSDGEWP
                     DGSDGEWP"
     gene            complement(342..1547)
                     /locus_tag="PKH10_03485"
     CDS             complement(342..1547)
                     /locus_tag="PKH10_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27221.1"
                     /translation="MTKYRLIALVPAIALLLMAGCDNTTILKPIPDNAPNDTDQTVVT
                     DDLATDDPVTDVPLTDADEQVPDGEWPDGEWPDGEWPDGEWPDGEWPDGEWPDGEWPD
                     SEWPDSDIEGACGSNANCKEQEYCAKPNGTCEAYLAGTCTVRPTEEECWMYSAIITVC
                     GCDNVTYQHPCFAQAAGVNVAYEGQCGTVTTCTTNEECGDFVAMHCQKAVGVCENGTG
                     VCVMPETGCAEIYAPVCGCDGNTYGNECTAHASFMNVAYEGECGGEEKFSTLSYYYDA
                     NTDEPPLATVIIYTADGTVTFEGADIVSREADNTYVYLRTTFYGPDGGGVVDLQLRLA
                     AGSQLPVTATLDGTNSYAQWTNFYGGGPIELIGNLYGAVTISQYQRNDNTITLIEMGG
                     KELTFEENR"
     gene            complement(1666..2172)
                     /locus_tag="PKH10_03490"
     CDS             complement(1666..2172)
                     /locus_tag="PKH10_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNT27222.1"
                     /translation="MTFKKGDLAVYPNHGVGEIVSIEEKALGADTVKCYVVNMVHSGS
                     KVFVPVDSASRMGLRNVVEKTSAATVYSCLHERNPLLLKMNWNKRYRYFQDKMRTGKA
                     TDVASVVSDLLYLKRKKGLSYGEEKMLHDAEEILATELSIAEKSDKEAIRKKIYRLVE
                     SSLKTPAR"
     gene            complement(2444..4429)
                     /locus_tag="PKH10_03495"
     CDS             complement(2444..4429)
                     /locus_tag="PKH10_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNT27223.1"
                     /translation="MARSLFALAMVAFPLMVGVSCSGGGGEAQDTDELTSDADTVPPL
                     ICRTPGVGPYPIVFTDVTDEIGFGETGLVVVGSVVTSADINNDGWPDLYISKGAKTRE
                     NSEVPTGLYRLFINDKGTGFTETTWTSGLFKRRDGEDGRVSSFVVWGDVNNDGNMDAF
                     NAAYFDADTDETGDQSSVFLGKGDGTFTIAPLHEFFTTYLDPVAGVSFLDYDRDGLLD
                     IFVGHHYGKYGYLNMAEQDTLHRGDGAGKFTHATDEAGLTTLKGTTDEIIKSGKNHKP
                     TWGVTACDVDGDGWTDLMTTTYGRGFNMFWRNKGGTFEDLSISSKFAEDEMLDYADDQ
                     WYLCYCKSHTDAGDYCADAASPSIDCTGLEEAWEPGFSDHPARLGGNSSATVCADIDG
                     DGDMDLLAVELAHWHIGQASDKTEILRNDGFPEKPFVRPGREATGILRTHVSGWNEGD
                     LGALADDIDNDGRIDLYVLSSDYPGTYALLYQQQADGTFAEKGSDAGVRVIRSHGATL
                     IDYDRDGDYDMVVGSSLMRWSASDVPPAPSQPWVKVLRNDTAPAANRFMFLLEGAGTV
                     DGTNRAAIGARITVKAGGKTFIREYQGGYGLFGFQNDPLIIVGAGEACVADEITVRWP
                     NKKGSSSTFNEIPANYVVRIHEKDGLSYQTPEEYAAR"
     gene            complement(4493..5548)
                     /gene="ribD"
                     /locus_tag="PKH10_03500"
     CDS             complement(4493..5548)
                     /gene="ribD"
                     /locus_tag="PKH10_03500"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNT27224.1"
                     /translation="MRTPLASLMARAVEIARNGGRAVYPNPMVGAVICDDEGNILSEG
                     WHRCCGGDHAEVDALKKLDRSATGLTMLVTLEPCNHFGRTPPCSHAIVAAGIKKVYIA
                     SHEPTEKACGGCDYLTSHGVAVEFVPGFEEELAELNRFFSATAKERRPWVTLKAATTA
                     DGFIAPPGGGRFAISSPEALVEGHRLRAGHMAIAVGAGTVNSDDPLLTVRLVEGPDPI
                     PVIFSARLALKPDRRVLDRAPIIFTAVADRHRVALLAARGCRIETLTPGAGSIGRALS
                     VLWERYRINSLLVEGGAALHTSFLAEGLVDEMVRFIAPLPLGEGIALAEPAEADRRFR
                     LHAVSPCGADVMQVWRR"
     gene            complement(5545..6543)
                     /locus_tag="PKH10_03505"
     CDS             complement(5545..6543)
                     /locus_tag="PKH10_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27225.1"
                     /translation="MRRPGGPYLPIFRIIPLSLLLYTVMLCAAELPVPAPAPDEALAP
                     SRREPGSGFFLSGTERGPRLETHLCGADAMRPGGQFYFFNKKKELCQRSVTYHPAAVA
                     IRLPRTAAPVSIELPWRLVDGEESILLYDPAAKFGELSDEEGRVLVEDEYGVLFDAAG
                     AVAAPRFLFRDGSWGPVVPLRELFLLQVQADRSFSVEVRSAADLSGATADIASFFGLS
                     AERIVLRSPGPHRATITGGGFSDMRIPMLVPDGKGDQFGRYRIEMRKSGPDRKGKRVR
                     RAELKFAAGAAIAETTLNGSFTLEITIRKEEMDPAEEEKLRLFLQQNLPRSYAEDR"
     gene            complement(6540..6902)
                     /gene="rplS"
                     /locus_tag="PKH10_03510"
     CDS             complement(6540..6902)
                     /gene="rplS"
                     /locus_tag="PKH10_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNT27226.1"
                     /translation="MVSPIIRDIEKRQMRVDHYFIKPGDKVSVHTRIIEGDKQRIQVF
                     TGDVIAINNGRANSRTTITVRKSSSGFGVERIFPLHSPLIEKIELINRGIVRRAKLYY
                     QRNRSGNAAKIKVKTDWR"
     gene            complement(6905..7489)
                     /locus_tag="PKH10_03515"
     CDS             complement(6905..7489)
                     /locus_tag="PKH10_03515"
                     /inference="COORDINATES: protein motif:HMM:NF021455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNT27227.1"
                     /translation="MERVSLALLHYPVRQGGRTITSSVTNFDIHDLARLAATYGLGAF
                     YLAHPDEGMRSLATEFVSHWFDGAGRLFNPDRTTALEKLHIVRTLDEAIADIERRHGE
                     RPFLLATSAVRRPKTLPWERLVALGDRPLLILFGTAEGISEELDDIIDGYADPIDPGT
                     GYNHLSVRSAASIFIDRLHSCKYKPLPFLRNEGV"
BASE COUNT         1386 a         2306 c         2370 g         1541 t
ORIGIN      
        1 gggccactcg ccgtccgaac cgtcgggcca ctcgccgtcc gaaccgtcgg gccattcgcc
       61 atcaggccat tcaccgtcat cggtaacgac ctgcccgtcg tcgttgatga cctgcccgtc
      121 atcattgatg atctggccgt catcattgat gagttggccg tcatcgttga taagttgacc
      181 gtcgtcgttg atgagttggc cgtcatcgtt cagcagttcg tcgccgccgg cgagttggtc
      241 attgtcggaa gggaactttt tgtatgtcgt gccgctctcg caggccgcga aaagggccag
      301 cacacagaat ccgaatacga acctcttcat ggtatcctcc gttatcggtt ctcctcaaag
      361 gtcagttctt ttcctcccat ctctatcagc gtgatggtgt tgtcgttccg ctgatactgc
      421 gatatggtca ccgcaccata cagattaccg atgagttcga tcggaccacc gccgtagaag
      481 ttggtccact gggcgtagga attcgtgccg tcgagcgtcg ccgtcaccgg cagttgacta
      541 ccggcggcaa gccgcaactg aagatcgacc accccgccgc cgtcgggccc gtagaaggtg
      601 gtccgcaaat agacataggt gttatcggcc tcacgactca cgatatcggc cccctcgaaa
      661 gtgaccgtcc cgtcggcggt gtaaatgatc accgtcgcaa gcggcggctc gtcggtgttc
      721 gcgtcgtagt agtaggacag ggtcgagaac ttctcttccc ctccgcactc cccctcatag
      781 gcgacattca tgaaactggc atgcgccgtg cactcgttgc cgtaggtgtt cccgtcacat
      841 ccgcagaccg gcgcatagat ctcggcacag ccggtctcgg gcatcacgca aacgccggtg
      901 ccgttctcgc atacgccgac cgctttctgg cagtgcatcg cgacgaaatc gccgcattct
      961 tcgttggtcg tgcaggtggt gacggtaccg cactggcctt cgtaggcaac attcactccg
     1021 gcggcctgcg caaaacaggg atgctggtag gtcacattgt cgcagccgca gacggtgatg
     1081 atggccgagt acatccagca ctcctcctcg gtcggtcgca ccgtgcaggt gccggccaga
     1141 taggcctcgc aggtcccgtt cggtttggca cagtattcct gctccttgca attggcgttg
     1201 cttccacaag cgccttcgat atcgctgtcg ggccactcgc tatcaggcca ctcaccgtcg
     1261 ggccactcac cgtcgggcca ctcaccgtcg ggccactcac cgtcgggcca ttcaccgtca
     1321 ggccattcac cgtcaggcca ttcaccgtcg ggcacctgct catcggcgtc ggtgagggga
     1381 acatcggtga cggggtcgtc ggtggccaga tcatcggtga caacggtctg atcggtgtca
     1441 ttcggcgcat tgtcggggat cggcttcaga atggtggtgt tgtcacagcc cgccatcagg
     1501 agcagggcga tcgccggcac aagcgcgatc aggcgatatt tcgtcatggg catcctcccg
     1561 aaatcaaaga ccggacgatt tagtactccc tgtgtgacgg ctttattata ccactattta
     1621 tttttttgca agtggtatgt tgtctgggga cgggagaaaa gcccttcagc gggccggggt
     1681 cttcagcgat gattcgacga ggcggtagat cttcttgcgg atcgcctcct tgtcgctctt
     1741 ctcggctatc gagagttcgg tggcgagtat ctcctcggca tcatggagca tcttctcttc
     1801 gccgtaggaa agcccttttt tccgtttgag gtacagcagg tcggacacca cggaggcgac
     1861 atcggtcgcc ttgccggtcc gcatcttgtc ctgaaaatag cggtaccgtt tgttccagtt
     1921 cattttgagc aggagcgggt tgcgctcatg caggcaggag taaacggtcg cggccgaggt
     1981 cttctcgacg acattccgca ggcccatgcg cgacgccgaa tcgaccggca cgaagacctt
     2041 tgaaccggaa tgcaccatgt tgaccacata acacttgacg gtgtcggcgc caagcgcctt
     2101 ctcctcgatg gacacgatct caccgacgcc atgattggga tagacagcca aatcgccttt
     2161 cttgaaagtc atcacgggaa cctccttcgc caagagatcc gtaaaaggga cgagagaacc
     2221 ttttgaatgt aacggcgaga gggtgcgaag tcaaggaatt tcttaaaata ctttgatttt
     2281 tcaatttgtt gcgctcgtta tataggccgt tatgtcatat aaaaagagag gtccgggaag
     2341 ggcaccgcct taaccgtgga cagctctttt atctttctct tcgggcagtt gactatttga
     2401 cggcataacg gatcgcttta ctttaaggta acggcgacga acatcagcgg gcggcatatt
     2461 cttcgggcgt ctggtaggaa agaccgtcct tttcgtgtat ccgcaccaca taatttgccg
     2521 gtatctcatt gaaagtactc gatgatcctt tcttgttcgg ccagcggacc gttatctcgt
     2581 cggccacgca ggcctcgcct gcgccaacga tgatgagcgg gtcgttctga aacccgaaaa
     2641 gcccataacc accttgatat tcacggataa atgtttttcc acccgccttg acggtgatac
     2701 gggcgcctat cgctgcccgg ttggtgccat cgaccgtacc ggctccttcg agaaggaaca
     2761 tgaagcggtt cgccgccggc gcggtatcgt tgcggagcac cttgacccac ggctgtgacg
     2821 gcgcgggcgg cacatcggag gcgctccaac gcatgagcga ggaaccgacg accatgtcgt
     2881 agtcaccgtc gcggtcatag tcgatgagcg tcgcgccatg ggaccggatg acgcggaccc
     2941 ccgcatccga gcccttctcg gcgaaggtgc cgtcggcctg ctgttggtac agcagagcat
     3001 aggtgccggg gtagtcggaa ctgaggacat agagatcgat gcggccgtcg ttgtcgatat
     3061 cgtcggcgag cgcaccgagg tccccttcgt tccaccccga gacatgggtc cgcaggatgc
     3121 cggtcgcctc gcggccgggg cggacgaacg gtttttcggg gaacccgtcg ttgcgcagta
     3181 tctcggtctt gtcggacgcc tgcccgatgt gccagtgggc cagttcgacc gcgagcaggt
     3241 ccatgtcgcc atcgccgtcg atgtcggcgc agacggtcgc cgaactgttg ccgccgagcc
     3301 gcgccggatg gtcgctgaag cccggctccc acgcctcctc gagtccggtg caatcgatcg
     3361 aggggctcgc ggcgtcggcg cagtagtcgc ccgcgtcggt gtgcgacttg cagtaacaga
     3421 gataccactg gtcgtcggca tagtcgagca tctcgtcctc ggcgaacttc gaggagatcg
     3481 agaggtcctc gaaggtgccg cccttgttgc gccagaacat gttgaagccg cgaccgtagg
     3541 tggtcgtcat gaggtcggtc cagccgtcgc catcgacatc gcacgcggtg acgccccagg
     3601 tcggtttgtg attcttcccc gacttgatga tctcatcggt ggtccccttg agcgtcgtga
     3661 gccccgcttc gtcggtcgcg tgggtgaact tcccggcgcc gtcgccgcgg tggagcgtat
     3721 cctgttcggc catgttgagg tagccgtatt tgccgtagtg atggccgacg aatatgtcaa
     3781 gcaggccatc gcggtcataa tcgaggaacg agacgccggc gaccggatcg aggtaggtcg
     3841 tgaagaactc gtggagcggg gctatcgtga aggtgccgtc gccctttccg aggaacacac
     3901 tgctctggtc gcccgtctcg tcggtgtcgg cgtcgaagta ggcggcgttg aaggcgtcca
     3961 tattcccgtc gttgttcaca tcgccccaga cgacgaacga ggagacgcgg ccatcctccc
     4021 cgtcgcgcct cttgaacagg cccgaggtcc aggtggtctc ggtgaaccca gtccctttgt
     4081 cgttgatgaa tagacggtag aggccggtcg gcacctcgga gttctcgcgg gtcttcgccc
     4141 ctttcgagat atagagatcg ggccagccgt cgttgttgat gtcggcgctc gtcacgaccg
     4201 agccgacgac cacgagtccc gtttcgccga acccgatctc gtcggtgaca tcggtgaaga
     4261 cgatcggata gggcccgacg ccgggggtgc ggcagatgag cggcggcacc gtatcggcgt
     4321 cggaggtcag ttcatcggta tcctgcgcct cgccgccgcc gccggaacag gataccccga
     4381 ccataagggg aaaagcgacc atcgcgaggg caaaaagaga ccgcgccatg acagatcctc
     4441 catcttttat gaacaggtca ccatcatacc gttaccgtga ggcaaagcaa ggttaccggc
     4501 gccagacctg catcacatcg gcaccgcacg gcgaaacggc gtgcaggcgg aagcgacggt
     4561 cggcttcggc cggctccgcc aacgctatcc cctcgccgag agggagcggc gcgatgaaac
     4621 ggaccatctc gtcgacaaga ccctcggcga ggaacgaggt gtggagcgcc gcgcctcctt
     4681 cgacaagcag cgaattgatg cggtatcttt cccaaagaac ggagagcgcg cggccgatgg
     4741 atcccgcccc cggagtcagc gtctcgatgc ggcacccacg cgcggcgagc agcgcaacgc
     4801 ggtggcggtc ggcgaccgca gtgaagatga tcggggcgcg gtcgagcacc cttcgatcgg
     4861 gcttgagcgc gagccgtgcg ctgaagatga ccggtatcgg gtcgggaccc tcgacgaggc
     4921 gcaccgtcag gagcgggtcg tcgctgttca ccgtaccggc gccgacggcg atggccatgt
     4981 gtccggcgcg cagacggtgt ccttcgacaa gcgcttcggg ggatgatatc gcgaaccggc
     5041 cgccgccggg cggggcgatg aaaccgtcgg cggttgtcgc cgccttgagc gtcacccacg
     5101 gccgccgttc tttcgcggtg gcggagaaga accggttgag ttcggcgagt tcctcttcga
     5161 accccggcac gaactctacc gcgacaccgt gcgaggtcag atagtcacag ccgccgcatg
     5221 ccttttcggt cggctcatgc gaggcgatat agaccttttt gatgcccgcc gcgacgatcg
     5281 cgtgactgca cggcggggtc ctgccgaaat ggttgcacgg ttcgagcgtc accagcatgg
     5341 tgagccccgt cgccgatcgg tcgagtttct tcagcgcgtc cacctcagcg tggtcgccgc
     5401 cgcaacaccg gtgccaccct tcggaaagga tgttcccctc gtcgtcgcat atcaccgcgc
     5461 cgaccatcgg gttggggtag acggcacgac cgccgttgcg ggctatctcc accgcccgcg
     5521 ccatgagcga ggcaagcggc gtcctcatct atcctccgcg taggaacggg gaagattctg
     5581 ttgtaggaag aggcgcagtt tctcttcctc cgccgggtcc atttcctctt ttcttatcgt
     5641 tatctccagc gtgaacgacc cgttgagcgt cgtttcggcg atggcggcac cggcggcgaa
     5701 cttcaactcc gcgcgtcgca ctctttttcc cttccggtca gggccgctct tccgcatctc
     5761 gatgcgatac ctgccgaact gatcaccctt cccgtcggga acgagcatcg gtatccgcat
     5821 gtcggagaat cctccaccgg tgatcgtcgc gcgatgaggt ccgggcgaac gcaggacgat
     5881 gcgttcggcc gagagcccga agaacgacgc gatgtcggcg gtcgcgcccg acaggtcggc
     5941 ggcgctccgt acctcgaccg agaaagagcg gtcagcctgc acctgcagga ggaacagttc
     6001 cctgagcggg acgaccggac cccacgagcc gtcgcggaac aggaagcggg gggcggcgac
     6061 ggcgcccgcc gcatcgaaca acacgccgta ctcatcctcg acgagcacgc gcccttcttc
     6121 gtcggacagt tcgccgaatt tcgcggcggg atcgtacagc aggatgctct cctcgccgtc
     6181 caccagccgc cacggcagtt cgatcgacac gggggcggcg gtgcggggca ggcgtatggc
     6241 gacggcggcg ggatggtagg tcacgctccg ctggcaaagt tcctttttct tgttgaagaa
     6301 atagaactgc ccaccggggc gcatcgcgtc ggcgccgcac agatgggtct cgagccgggg
     6361 gcctcgctcg gtgccgctca gaaagaagcc ggaccccggc tcccggcgcg acggagcgag
     6421 cgcctcatcg ggcgccggcg ccggtaccgg aagttccgcg gcgcacagca tgacggtata
     6481 gagcagaagc gacagaggaa tgatacggaa gatgggaagg tacggtccgc cggggcgtct
     6541 caccgccagt cggtcttgac ctttatcttg gcggcgttac cgctgcggtt gcgctggtag
     6601 tagagtttgg cgcgccggac gatgccgcgg ttgatcaatt ctatcttctc gatgagcggc
     6661 gaatggagcg ggaatatgcg ctcgacgccg aaccccgaag aggatttgcg taccgtgatg
     6721 gtggtgcggc tgttcgcgcg gccgttgttt atcgcgatga catcgccggt gaacacctgt
     6781 atgcgctgtt tgtcgccctc gatgatgcgg gtatggaccg acaccttgtc accgggcttg
     6841 atgaagtagt ggtccacgcg catctggcgc ttctcgatgt cgcggatgat cgggcttacc
     6901 atggtcaaac tccttcgttc ctcaaaaacg gaagaggttt atacttacaa gagtgaagtc
     6961 tgtcaataaa aatcgatgcg gcgcttcgga cactgagatg gttgtatccg gtaccggggt
     7021 cgatcggatc ggcataacca tcaatgatat catcaagttc ttcgcttatt ccctcggcgg
     7081 tcccgaacag gataaggagc ggtcggtccc ccagcgcgac gagtcgttcc cacgggaggg
     7141 tcttgggccg ccgcaccgcc gaggtcgcaa gcaggaaggg gcgttcgccg tgacgccgct
     7201 cgatgtcggc gatcgcctca tcgagtgtcc ggacgatatg cagtttttcg agcgcggtgg
     7261 tccggtcggg attgaacagt cgcccggcgc cgtcgaacca gtggctcacg aactcggtcg
     7321 ccagagagcg catcccctcg tcgggatggg cgagatagaa ggcgccgagc ccgtaggtcg
     7381 ccgcgagccg cgcaaggtca tggatatcga agttggtgac cgaactcgtg atggtgcgcc
     7441 ccccctgccg gaccgggtag tgcagaagcg cgagcgagac ccgttccatt ggtcacttgt
     7501 cgtcgtcgaa gaagttctcg ggcaggagcg ccagcagttc tggacggttg cggagcgtcc
     7561 gcatgagcgc gttctttttc cgccacgcct ctatcttcgc atg
//
LOCUS       DAOQUE010000075         7537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_10778_length_7537_cov_3.845362, whole genome shotgun sequence.
ACCESSION   DAOQUE010000075 DAOQUE010000000
VERSION     DAOQUE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10778_length_7537_cov_3.845362"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKH10_03520"
     CDS             <1..516
                     /locus_tag="PKH10_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27228.1"
                     /translation="LTPSLTYAHLLDEHGKIGAITRHSLYAAFLTDARYSLGGEEKDP
                     ELINLDTFIYGSSSAVTVRGYRSGALTGRTVYFTNNELRFHVLSIEEGLGLFPLMVKN
                     LQGAVFCDLGAGAGDLALRERSFIVSLGVEGKLLIHWWYHVPLMFKLGLARGLTEGGV
                     WNVYVAVGNSF"
     gene            585..1814
                     /gene="pepT"
                     /locus_tag="PKH10_03525"
     CDS             585..1814
                     /gene="pepT"
                     /locus_tag="PKH10_03525"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNT27229.1"
                     /translation="MHHGVLERFLRYIAIDTQSDENSETSPSSAKQFDLAKLLKKELE
                     EMGLKNVSLDEYGYVMAELPSNIDKKVPAIGFIAHMDTSPDMSGANIKPQTLKNYDGG
                     DITLNKGLGITLSVKEFPELKMYKRETLITTDGTTLLGADDKAGIAEIMTAMEYLVKH
                     PEIKHGPIKIGFTPDEEVGRGVDHFDVKKFGADLAYTMDGGMIGELEYENFNAAMASI
                     TIQGRNIHPGYAKDKMKNAIIIGMELNDLLPVDQRPEFTVGYEGFYHIMKFEGTVEKA
                     TVKYIIRDHDRAKFEAKKVFITRAVEFLDQKYGSGVVKLELKDQYFNMREKVEPVFHV
                     VETAKKAMELCGVEPHVVPIRGGTDGSRLSYMGLPCPNIFAGGHNFHGKLEYVVVGSM
                     EKAVAVILKIVELYAAK"
     gene            complement(1818..2654)
                     /locus_tag="PKH10_03530"
     CDS             complement(1818..2654)
                     /locus_tag="PKH10_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27230.1"
                     /translation="MTPGGPFVRIDRDEEVRWHRFTGEKVTDLRMRYPGSVPIVYHER
                     SDLLVAKSLSGDGIRLTIYRGGSGPVKEEVTYNTTADEILSACITDAEGVWVLHQGRY
                     ESEAVRQFIVSRTTARFSEWENYFLAGVEVSEQGTVIEKPVNIFCGRDDTELYLSTVL
                     YAGNDRRSVLRLPTLSVLQAYLYQFDPVEGLLSPVTAVITRGTGRIRDRYDAVSGRYY
                     LFQGGLLTIQEGWGFPEEHRYEGAEDALLAVKEGTVRLMLIERSGKKVRGRIAPLSMR
                     GD"
     gene            complement(2770..4140)
                     /locus_tag="PKH10_03535"
     CDS             complement(2770..4140)
                     /locus_tag="PKH10_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27231.1"
                     /translation="MKRLLIVGLCVLFTLPLFGKKILFMEPIIKDVGSESKITSEIEK
                     VFKKKYDYVSYEDVKSKLMSKLKKCGSKTDCWAEEAGDADFDYAALFLIKDDDGEVLV
                     RLMVIDIDGEDSVADEKENFDSARDITAGSVYKKLMKKALSKISKSISKSSSRDEDED
                     EDDGGASAKEREREKREAALREKERKEEERKQRLEEERQRREEELERKREEEERKRRI
                     AEEKRREKEERERARLEEERRRREQEREEENAKKRDQLERNAEKLEKARNIVLDMCSK
                     GKYNEAIQAILKVSQLKCECEEDAKVLALKSQLLNFNKIRDKILEGINLLNYSLILDN
                     LEAAKALDTEITPGGTEFSQKIDRIYAIGYYAKALDMEKKDNYVVANENYEKCLQKDP
                     DKAECKKWFDEKDKTVKKLFDKAKVMKSFNPTKAKDLFRSILKLVTAENEYYKQSEQE
                     LKSMEY"
     gene            4804..5157
                     /locus_tag="PKH10_03540"
     CDS             4804..5157
                     /locus_tag="PKH10_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT27232.1"
                     /translation="MKKEARFFKALADDTRLAILALLSWREELCVCDIMTVLELTQSK
                     ASRHLRYLTQSGILDDERRTVWVYYRISPDLSPLEAGIIRDVQNHFNSKGYAALKKKL
                     EVWLAKKGGSPLCKD"
     gene            5178..5411
                     /locus_tag="PKH10_03545"
     CDS             5178..5411
                     /locus_tag="PKH10_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNT27233.1"
                     /translation="MKKLQVLGTGCPKCKQLAMLTEEAAKELGIEYELIKVTDINEII
                     DFGVMSTPALVVDDVVKVAGKVPPVGELKKLIA"
     gene            5422..5898
                     /locus_tag="PKH10_03550"
     CDS             5422..5898
                     /locus_tag="PKH10_03550"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNT27234.1"
                     /translation="MHAKKIVTILLVLFAAAGVGYAIFGGKAPVQKDSPPETPGAVTA
                     SDGLTVYYFHTTFRCHSCLTIERLTHETLNEQFAVEQKSGAIRWRMVNYEEPANEHFI
                     DDYKLYTKQVILSAVKGGKEVKWKNLDEVWDLKRDEMRFKLYIADEIKKFRAENGL"
     gene            5895..6572
                     /locus_tag="PKH10_03555"
     CDS             5895..6572
                     /locus_tag="PKH10_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNT27235.1"
                     /translation="MTVATILGALWLGILTSISPCPLATNIAAVSYISRTIGDKRSVY
                     LSAALYTLGRMFAYLAIAFIVVQSLLSIPKVSIWLQIYMPKLLGPLLIVVGLFLVELI
                     SVNLPGTKSQRDLSKFGLAGAVLMGFLFALALCPVSAALFFGSLIPLAIQQSSPVLLP
                     LIYGIGTALPVIAFAVAISLGSAALATAFNAVTRWEKRLRIAFGVIIIVVGIYLTIVH
                     VFGIPFI"
     gene            6586..>7537
                     /locus_tag="PKH10_03560"
     CDS             6586..>7537
                     /locus_tag="PKH10_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNT27236.1"
                     /translation="MNWKSEIKPFGLILAVFFAAFFLPVGVHRFDNALTEALELVKWY
                     AREHVLLCLVPAFFIAGAIGVFVSQNAVMKYLGAGAKKWVAYLVASVSGTILAVCSCT
                     ILPLFAGIYKRGAGLGPAIAFLYSGPAISVLSIILTARILGFEIGVARAVGAIGFSIV
                     IGLIMAFFFRKEEAERTAGIQLPPEEARRPLYQNIVYFFLMVAILVFANWGRPDVQEG
                     AWWAIFQMKWIVTAVAAMLFGVVLVVWYALKAWKIVVTGVVAALFAFLFPHEPLFAFS
                     AGFIGMTWSASTADGESGEWMSASWGFAKQILPLLFFGVLL"
BASE COUNT         1532 a         2270 c         2046 g         1689 t
ORIGIN      
        1 ctcaccccgt cgctcaccta tgcccacctg ctcgacgaac acgggaagat cggtgccata
       61 acacgccatt cgctctacgc cgccttcctc accgacgcgc gctacagtct cggcggcgag
      121 gagaaggacc cggaactcat caatctcgac accttcatct acggctcttc gtccgccgtc
      181 acggtgcgcg gctaccgctc cggcgcgctc actgggcgca ccgtctattt caccaacaac
      241 gaactaaggt tccatgtact cagtatcgag gagggactcg ggttgttccc gctgatggtg
      301 aagaacctgc agggagcggt gttctgcgat ctgggcgccg gcgccgggga cctcgccctg
      361 cgtgaacgct ccttcatcgt atcgctcggc gtagagggta aactcctcat ccactggtgg
      421 tatcatgtcc cgctcatgtt caaactcggc ctcgcgcgcg ggcttaccga gggcggcgtc
      481 tggaatgtct atgtcgcggt gggcaacagt ttttgattga cttgccccgc tcgctctgct
      541 acggtggaac ggcacgacga caaaccactt cgacggaggc ttccatgcac cacggcgttc
      601 ttgaaaggtt cctgcgttac atcgccatcg acacgcagtc cgacgaaaat tcagagacct
      661 ccccctcctc ggctaaacaa ttcgacctcg ccaaactcct gaaaaaagaa ctggaggaga
      721 tggggctgaa gaatgtctct cttgacgagt atggctatgt gatggcggaa ctgccgtcca
      781 acatcgacaa gaaggtcccg gccatcgggt tcatcgccca catggacacg agcccagaca
      841 tgtcgggggc gaacatcaag ccgcagacac tgaagaacta tgacggtggc gacatcacgc
      901 tcaataaagg gctgggcatc accctgtcgg tcaaggaatt ccccgaactc aagatgtaca
      961 agagggagac gctcatcacg accgacggca cgacgcttct cggcgccgac gacaaggcgg
     1021 gcatcgccga gatcatgacc gccatggaat acctcgtgaa acatcccgag ataaaacacg
     1081 ggccgatcaa gatcggcttc acccccgacg aagaggtcgg gcgcggcgtc gatcatttcg
     1141 atgtcaagaa gttcggcgcc gacctcgcct acaccatgga cggcggcatg atcggcgaac
     1201 tcgagtacga gaacttcaac gcggcgatgg cgagcatcac cattcaggga cgcaacatcc
     1261 accccggcta tgccaaggac aagatgaaga acgccatcat catcggcatg gaactcaacg
     1321 atctcctgcc ggtcgaccag cgacccgagt tcaccgtcgg ctatgaaggg ttctatcaca
     1381 tcatgaaatt cgaagggacg gtcgagaagg ccaccgtgaa atacatcatc cgcgaccatg
     1441 accgggcgaa attcgaggcg aaaaaggtgt tcatcacccg cgcggtcgaa ttcctcgatc
     1501 agaaatacgg ctccggcgtc gtcaaactcg aactcaagga tcagtacttc aacatgcgcg
     1561 aaaaggtcga gccggtgttc catgtggtcg aaacagcgaa aaaagcgatg gaactgtgcg
     1621 gcgtcgaacc gcatgtcgtc ccgatacgag gcggtaccga tggttcgcgc ctctcctaca
     1681 tgggtctccc gtgccccaat atcttcgccg gcggtcataa tttccacggg aaactcgagt
     1741 atgtggtggt cggatcgatg gaaaaggcgg tcgccgtaat actcaagata gtcgaactct
     1801 acgccgcgaa atagccccta atcccctcgc atcgacaggg gggctatccg tccccgcacc
     1861 tttttaccgg agcgttcgat gagcatgagc cgcaccgtcc cctctttcac ggcgagcaac
     1921 gcatcttccg ctccctcgta acggtgctcc tcgggaaatc cccatccctc ctgtatcgta
     1981 agaagacctc cctgaaaaag gtagtaccgc ccagaaacgg cgtcgtatcg atcccgtatc
     2041 cgtcccgtcc cacgcgtgat gacggcggtg accggcgaca agagaccctc caccgggtcg
     2101 aactggtaga gatatgcctg caggaccgaa agggtcggga gacgcaggac gcttcgccgg
     2161 tcgttgccgg cgtagagaac ggtcgaaaga tacagttcgg tatcgtcccg accacagaag
     2221 atattcaccg gcttttcgat gacggtcccc tgctccgaca cctcaacgcc ggcaaggaaa
     2281 tagttttccc attcggagaa acgcgcggtc gtccggctca cgatgaattg gcgtaccgct
     2341 tcgctctcgt agcgcccctg atggaggacc cacacgcctt ccgcatcggt gatgcaggct
     2401 gacaatatct cgtccgctgt cgtattatag gtgacctcct ccttcaccgg accggagccg
     2461 ccgcgataga tggtcaagcg aatgccgtcg cccgaaagcg atttcgcgac aaggaggtcg
     2521 gatcgttcgt gatacactat cggcacgctg ccgggatacc gcatccgaag gtccgtcacc
     2581 ttttcgcccg taaagcgatg ccagcgcacc tcttcgtcgc ggtcgatccg cacgaaggga
     2641 ccgccgggcg tcatgatctg acaatcctcc tcgcaggata tcttttctcc ggaacaggac
     2701 cccaaaagcg ctacgaaaga aaagagaaga aggagatatc ggggaaaggg gcgaagcacc
     2761 gtgcgtgctt cagtattcca tcgacttaag ttcttgttcc gactgtttgt agtactcatt
     2821 ctcggccgtg accagtttca ggatcgaccg gaacaggtcc ttggccttcg tcgggttgaa
     2881 actcttcatg accttggcct tatcgaaaag tttcttgacg gtcttgtcct tctcgtcgaa
     2941 ccacttcttg cactccgcct tatcgggatc cttctggagg catttctcat agttctcgtt
     3001 cgccacgaca taattgtcct tcttttccat gtcgagcgcc ttggcatagt agccgatggc
     3061 gtagatgcgg tcgatcttct gcgagaactc ggtgccaccg ggggttatct cggtgtcgag
     3121 cgccttggcc gcctcgaggt tatcgagaat gagactgtag ttgagcaggt tgatgccttc
     3181 gagtatcttg tcgcgtatct tgttgaaatt gagcagttgg ctcttgagcg cgaggacctt
     3241 ggcgtcctcc tcgcactcgc acttgagctg gctgaccttg agtatcgcct gtatcgcctc
     3301 gttgtacttg cccttgctgc acatgtcgag gactatattg cgcgcctttt cgagtttctc
     3361 ggcgttacgt tccaattgat cacgcttttt ggcgttctcc tcttcccgtt cctgttcgcg
     3421 gcgacggcgc tcctcttcga ggcgggcccg ctcgcgctct tccttttcgc gccgcttttc
     3481 ctcagcgatg cggcgtttgc gctcttcctc ttcgcgtttc cgttccaatt cctcctcgcg
     3541 gcgctgacgc tcttcctcaa gccgttgttt gcgttcttcc tctttgcgct ccttctcgcg
     3601 caatgccgct tccctcttct cgcgctctct ttcctttgcc gatgcgccgc catcatcctc
     3661 gtcctcgtcc tcgtcacggg aagacgactt gctgatgctt tttgatattt tggaaagagc
     3721 cttcttcatg agtttcttat aaacactgcc cgctgtgatg tcgcgtgccg aatcgaaatt
     3781 ctccttctcg tccgcgacgg aatcctcacc atcgatatcg atgaccatca gccgcaccag
     3841 cacctcgccg tcgtcgtcct tgatgaggaa aagggcggcg taatcaaaat cggcatcacc
     3901 cgcctcttcg gcccaacagt cggtcttgct gccgcatttc ttgagtttgg acatcaactt
     3961 ggacttgacg tcctcatagg aaacatagtc gtatttcttc ttgaagacct tttctatctc
     4021 ggaggttatc ttgctctcac tgccgacatc cttgatgatg ggttccatga agagtatctt
     4081 cttgccgaac agcggtaggg tgaagagtac gcacagcccg acgatcaaaa gccttttcac
     4141 agccgctcct cctgacctaa atctcacaaa atgtctggac cattaaatat gcttttattc
     4201 ttttttcaag tttttgtcct tattctttca ttgttttttg tttttcccgc cacattccca
     4261 tacgagaaga tgtgtcgctc cctcacgcat aatgccgcga cagggactct cgcctcccct
     4321 gaagagagcc ttttcgatac gcaaccactt ttcctcctcc cggacaatga actcgacccc
     4381 ctcgacgagt tcatctccga tcctgaccga ccccagagcc cggagccgtt ctttttcatt
     4441 ccgcaccaag atattctgag ggatggtcgt gaccggggtc gaagaggtgt tctcggtggc
     4501 gccggagccg aaggatctac cgatgcccca aaaaacagat gacgcttctc ctctccgtgg
     4561 ctccgcctca ctttgccgac gcacatggat gagatagaga aagatcgaga atgagagcaa
     4621 aagaagaacg aggaaaagtg cgcgtgcccg gtcgcccgat ccgctcatcg gtctgctatc
     4681 aactccctcc attcacgaat accgccccct gataccagag aacggacgga aaagcaactt
     4741 gactttgttt ttctcaacct tatactcgca taaacggtta tttgatactc ggaggttttt
     4801 cccgtgaaga aagaggcgag atttttcaag gcactggccg acgatacgcg gctcgcgata
     4861 ctcgcgcttc tctcgtggcg cgaggaattg tgcgtctgtg acatcatgac ggtgctcgaa
     4921 ctcacccagt cgaaagcgtc gcggcacctg cggtatctca cgcagtcggg cattctcgat
     4981 gacgaacggc gcaccgtgtg ggtctattat cgtatctctc ccgatctgtc gcccctagag
     5041 gcggggatca tccgggatgt gcagaaccac ttcaactcga aaggctacgc cgcgctgaaa
     5101 aagaaactgg aggtgtggct tgccaaaaaa ggcggctcgc cgttatgcaa agactgacaa
     5161 caccgtagga ggcgacgatg aagaaactgc aggtgctcgg gaccggctgt ccgaaatgca
     5221 agcaactggc gatgctgacc gaagaggcgg cgaaggaact cggcatcgag tatgaactga
     5281 tcaaggtgac cgacatcaac gagatcattg atttcggcgt gatgtcgacc ccggcgctcg
     5341 tcgtggacga tgtggtaaag gtggcgggga aggtgccgcc ggtcggcgaa ctgaagaaac
     5401 tcatcgcctg aaaggttccc gatgcacgcg aaaaagatcg tgacgatact gctggtgctg
     5461 ttcgcggcgg cgggggtcgg gtatgccatt ttcggcggaa aagcgccggt gcaaaaggac
     5521 tcgccgcccg aaacgccggg cgcggtcacc gcatccgacg ggctgaccgt ctattatttc
     5581 cacaccacct tccgctgtca ttcctgcctt accatcgagc ggctgaccca tgaaacgctg
     5641 aacgaacagt tcgcggtcga acagaaaagc ggcgcgatcc gctggcggat ggtgaactac
     5701 gaagaaccgg cgaacgagca cttcatcgac gactacaaac tctacaccaa acaggtcatc
     5761 ctttcggcgg tgaagggggg caaagaggtg aagtggaaga acctcgatga ggtatgggac
     5821 ctcaaacgcg acgagatgcg attcaaactc tacatcgccg acgagataaa gaaattccgc
     5881 gcggagaacg ggttatgacc gtcgcgacga tactcggagc actgtggctc gggatactca
     5941 cctcgatcag cccctgtcca ctcgccacca atatcgcggc ggtctcctac atctcgcgca
     6001 ccatcggcga caagcggtcg gtctatctgt cggcggcgct ctacacgctg ggcaggatgt
     6061 tcgcctatct ggcgatcgcc tttatcgtgg tgcagagtct cctctcgata ccaaaggtgt
     6121 cgatctggct tcagatttac atgccgaaac tgctggggcc gctccttatc gtggtcgggc
     6181 tgttcctcgt cgaactcatc tcggtgaacc tgccggggac gaaaagccag cgcgatctct
     6241 ccaaattcgg ccttgccggc gcggtgctga tggggttcct cttcgccctt gcgctctgcc
     6301 ccgtttcggc ggcgctcttt ttcgggtcgc tcataccgct cgcgatccag cagtcgtcgc
     6361 cggtgctatt gccgctcata tacggcatcg gaacggcgct tccggtcatc gccttcgccg
     6421 ttgcgatctc actgggcagc gcggcgctcg cgaccgcatt caacgccgtc acccgctggg
     6481 agaagcggct acggatcgcc ttcggcgtca tcatcatcgt ggtcggcatc tatctgacca
     6541 tcgttcatgt gtttggaata ccgttcatct aaggggtcgg atataatgaa ttggaaaagt
     6601 gaaatcaaac cgttcggcct catcttggcg gtgttcttcg ccgccttttt cctgccggtc
     6661 ggcgtccacc ggttcgacaa cgcccttacc gaggcactcg aactggtgaa atggtatgcg
     6721 cgggagcatg tgctcctctg cctcgtcccc gcctttttca ttgccggggc catcggcgtc
     6781 ttcgtctcgc agaacgcggt gatgaagtat ctcggcgcgg gggcgaaaaa atgggtcgcc
     6841 tatctcgtcg cgtcggtctc ggggacgata cttgccgtct gttcctgcac gatactgccg
     6901 ctcttcgccg gcatctacaa acggggcgcg ggactcggcc ccgccatcgc attcctctat
     6961 tccggcccgg cgatcagcgt actgtcgatc atcctgacgg cgcgcatcct cggcttcgag
     7021 atcggcgtcg cgcgggcggt cggcgcgatc ggcttctcaa tcgttatcgg actcatcatg
     7081 gcattctttt tccggaaaga agaggcggaa cggaccgccg ggatacaact gccgcccgaa
     7141 gaggcgcggc gaccgcttta ccagaacatc gtttactttt tcctcatggt cgccatcctc
     7201 gttttcgcca actgggggcg gcccgatgtg caggaggggg catggtgggc gatattccag
     7261 atgaaatgga tcgtcaccgc cgtcgcggcc atgctgttcg gcgtcgtgct cgtcgtctgg
     7321 tatgccctca aggcgtggaa gatcgtcgtg accggcgtcg ttgcggcgct cttcgcgttc
     7381 ctttttccgc acgaaccgct cttcgccttc agcgccggct tcatcggcat gacatggtcg
     7441 gcctcgaccg ccgacgggga aagcggtgag tggatgtcgg catcgtgggg cttcgccaaa
     7501 cagattctcc ccctgctctt cttcggcgtg ctgctcg
//
LOCUS       DAOQUE010000076         7429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_10980_length_7429_cov_4.433143, whole genome shotgun sequence.
ACCESSION   DAOQUE010000076 DAOQUE010000000
VERSION     DAOQUE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10980_length_7429_cov_4.433143"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1679
                     /gene="rpoN"
                     /locus_tag="PKH10_03565"
     CDS             150..1679
                     /gene="rpoN"
                     /locus_tag="PKH10_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNT27237.1"
                     /translation="MTGLLKQEIVQRQIQTLNITQEMRLRIELLQKNLLDLREMLIRE
                     SEENPFLEIVEWGDESPGASATGNDGDEDESAPTPASGDRPERLGEENPRTMSDILAD
                     YDWRNHQEHSGNNFGDTRVRRRNDEPDENPIEETVSEKEPVTRRLDLQIAATEIPDRI
                     KETMIYMVYNLDDRGFLRDTDDDIASTLEIPVDEVREARAALRLLDPAGTGSFDLPDY
                     LRFIFRDPEQLAEPDISADLIRKLVDSPALLDALGKKDFARLCQELGIGKQQLGGILT
                     IMRKVAPYPFFGHETFTPEQVIPDLRVHLVEGEVVIEIERKFIPSIQLNRELYDQKVK
                     SIRDKQKRSLMRERFRAAEWLVKSLSERNKTLYEVAASLFNFQRAFLENGPDFLRPLT
                     LKDISEDIGRHQSTVSRLTNGKYAVTPFGIFELKSFFVKRINEESLATTNRHLETRLR
                     ELIAAEDPASPLSDEDLVRILSHEGVAIARRTIGKYRDKLRIPSARERRRDHEFLSGG
                     D"
     gene            1685..2641
                     /gene="hprK"
                     /locus_tag="PKH10_03570"
     CDS             1685..2641
                     /gene="hprK"
                     /locus_tag="PKH10_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNT27238.1"
                     /translation="MPSTTPVNIKQLIDTSDLSLTRENLDDQALEKREVKDSRVQVVG
                     LSIAGFTDHLYRERIQLLGKSENDYLNTLGDAERSARIESFLACHPVAVIFTDPVRPS
                     PEFNARADAHEVPLLSTRMVSSIFIDFIREHLNRLLAASATLHAQLLDVFGVGMLIMG
                     ESGVGKSETSLDLVMRGHKLIADDVVEIRFIPPDRLVGACNEMMRDLIEIRGIGLINV
                     QRMFGVTSVNYEKQIDCIINLVLWDQSATFEYERIGATTRHYEILGVKRPYYIIPVRH
                     GSNLSSLMEVIARDHMLRSQGVNVAAEFEQNLAKKLSEMGGA"
     gene            2641..3066
                     /locus_tag="PKH10_03575"
     CDS             2641..3066
                     /locus_tag="PKH10_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27239.1"
                     /translation="MFSFIFVTHGDLGASLVRTAEFIMNERFDDRCETFSLDYSMLAD
                     MDRIKELLERSVDRIVTKGHRAIVFVDIFGGSPSNVAFTFAKRDEVDILSGVNLPMVI
                     YAFEHRDAPVSIAELTAGILQSGDESLISAKKLLEKEHR"
     gene            3071..3340
                     /locus_tag="PKH10_03580"
     CDS             3071..3340
                     /locus_tag="PKH10_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNT27240.1"
                     /translation="MREHTVTLQYRKGIHARAASLFAKEAERFSSKVELEKNGVRINA
                     KSITGILMLKIPFGATFKIIVNGPDEDECLTTLVQLAESRFEEEL"
     gene            3340..5130
                     /gene="ptsP"
                     /locus_tag="PKH10_03585"
     CDS             3340..5130
                     /gene="ptsP"
                     /locus_tag="PKH10_03585"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNT27241.1"
                     /translation="MAPEKRPVLLPGEKIVEGIAIGKVFFIDKDFSLIPHLTLRGGQK
                     AREKERRRLTAAVKKCEKELVSIMEERHLPKEATSILEAHRMMLVDPELLRKIGETIM
                     DRAVNAEWAVLLVFDEMMAHLSSSDGDYYLRAKVADLEVIRDKLLFSLLGGAGRTSYI
                     KDLPTEDFVLCAHSLTLSDLSSLAKDPHLRGIALETPGGVSHLTIVLRSLGLPTVLGA
                     DGLLTEVVGCDEAVVDGLAGRVVLSPSPADKSAYLKKKQAYDDYFDRFLHNAGRPALS
                     RDGSMLHIGANIENVEDVEPSRRCGADFIGLFRTELMFLEKGDIPTEDDHYAVYYEML
                     HRAHPMEVTIRVFDFGGDKEGGIHATGAMGLRGIRFCFLYPDVFLPQVRGLIRAASLG
                     NLRILLPFVSAVDEIHEFKAILKEQAKELKLEKNLRHIKIGAMIELPAALFIAEMLSR
                     EVDFFSIGTNDLVQYLMAVERQDKALSQYFSHFHPAVLRALYNLHHIAKKARIDLSVC
                     GEMGGDPYFTLLFMGMGIHALSMTPLAIPMVKNIIRSSSVEEGRALLNRLLMTASEME
                     LRETLEKEMEQRYPDIFRKEWLDNLKKNGG"
     gene            5132..6034
                     /locus_tag="PKH10_03590"
     CDS             5132..6034
                     /locus_tag="PKH10_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNT27242.1"
                     /translation="MALTKLDTLLEMARGKKTSRLVVAAAEDEPVLEAVRDAMKEKII
                     VPILVGDAPKIKEIAAKVKLSLDGIEIIDEKSPAKSSKIAVQCIREGKGDIMMKGLVG
                     TADLLRAVLDKENGLRKGEIISHVALHEVKGYHKLLMVTDAALTPAPTFAQKVDMIHN
                     AAEVYHKLGIKEPKVAVLCAAETVSEKIEASVHAAMLTTMNRRGQIKGAIVDGPLALD
                     NAVSKEACEHKKIKSEVGGDADILVCPDIEAANILYKALNFLAGATTAGVLMGAKVPI
                     VLTSRADTDRSKLASIALAAAMEL"
     gene            6051..7121
                     /gene="buk"
                     /locus_tag="PKH10_03595"
     CDS             6051..7121
                     /gene="buk"
                     /locus_tag="PKH10_03595"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNT27243.1"
                     /translation="MRILAINPGSTSTKIAVFEENKEIFTKNLKHTVEELAPYKKIID
                     QYDFRRDVIVKELEAAKIPLSSITAVVGRGGLVKPIPGGVYRVSDALIADLKAERMGE
                     HASNLGGLIAREMARKIGPKVDSFIVDPVVVDEMEDIARISGIPELPRVSIFHALNQK
                     AVARRYAREQGKRYEELNLVVVHMGGGISVGCHKKGRVVDVNNALDGDGPFSPERSGG
                     VPVGQMVKLCFSGKYTIDDIKKKIKGKGGLVAYLGTNDAYEVEQRVQKGDKEAEHVYR
                     AMAYQIAKEVGALSTVLCGKIDAVILTGGVAYDKIFVGWLTERIGFLAPVVVYPGEGE
                     MTALAEAGYYAMKGELPIQDYV"
     gene            7174..>7429
                     /locus_tag="PKH10_03600"
     CDS             7174..>7429
                     /locus_tag="PKH10_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27244.1"
                     /translation="MLLLAIGCTTAPVDIDGPSPVSPAVAATPLSRVETPTEGTLLRG
                     SDPRMLMFRAFLDRAGIPVLFGVDGAGLFVLSVARDPTDVF"
BASE COUNT         1614 a         2342 c         2162 g         1311 t
ORIGIN      
        1 ctaggggaca taatcgcttt gcagttaggg ggacattttg actttgcttc aacacaaatg
       61 tcctcaataa ttttaacata agcaatattc ttgccgggga catccttttt cgctatagtc
      121 cccccgaaga gacggaggat gccgcttcaa tgaccggcct gctcaaacag gagatcgtac
      181 aacgacagat acagacgctc aacatcacac aggagatgcg cctgcgcatc gaactgctcc
      241 agaagaacct gctcgacctc cgtgagatgc tgatacgcga atcggaggag aaccccttcc
      301 tcgagatagt ggaatggggc gacgagtctc ccggagcgtc ggcgaccggc aatgacggcg
      361 acgaggatga gagcgctccc acgccggcat cgggagaccg tcccgaacgg ctgggcgagg
      421 agaacccccg gaccatgtcc gacatcctcg ccgactacga ctggcgcaac catcaggagc
      481 acagcggcaa caacttcggc gacacgcgcg tccggcgacg caacgacgag ccggacgaga
      541 acccgatcga ggaaacggta tccgagaagg agccggtcac ccggcgactc gacctgcaga
      601 tagccgcgac cgagatcccc gaccgcatca aggaaacgat gatctacatg gtctacaacc
      661 tcgacgatcg cggttttctg cgcgacaccg acgatgatat cgcctcaacg ctcgagatac
      721 cggtcgatga ggttcgcgag gcccgggcgg ccctgcgatt gctcgacccc gcggggaccg
      781 gctcgttcga cctgcccgat tatctgcgat tcatcttcag ggacccggaa cagctggcgg
      841 aaccggacat atccgcggac ctcatccgga aactggtcga ttcccccgcg ctgctcgatg
      901 cgctcgggaa aaaagatttc gcgcggctct gtcaggaact cggcatcggc aagcagcaac
      961 tcggcgggat ccttacgatc atgcgaaagg tggcccccta ccccttcttc ggccacgaga
     1021 ccttcactcc cgaacaggtc attccggatc tccgcgtcca tcttgtcgag ggggaggtgg
     1081 tcatcgagat cgagcggaaa ttcatcccct ccatacaact caaccgcgaa ctgtacgacc
     1141 agaaggtgaa aagcatccgg gacaagcaga aacgatccct catgcgcgaa cggttccgcg
     1201 ccgccgaatg gctggtcaag agcctttcgg aacggaacaa gacactgtac gaggtcgcgg
     1261 caagcctgtt caacttccag cgcgccttcc tcgagaacgg tcccgatttc cttcgtccgc
     1321 tcacgctgaa ggacatatcg gaggacatcg gacgccatca gtcgaccgtc tcacgactca
     1381 ccaacggcaa atacgccgtc acgcccttcg gtatattcga actcaaatcc ttctttgtca
     1441 aacgaataaa cgaggagtcg ctcgccacga ccaaccgcca cctcgagacg cgactcaggg
     1501 aactgatagc cgccgaggat ccggccagcc cgctttcgga cgaggatctc gtgcgcatcc
     1561 tctcccacga gggggtcgcc atcgcgcggc ggaccatcgg gaaatatcgt gacaaactgc
     1621 gcataccgtc ggcacgcgaa cggcggcgcg accatgaatt cctttcggga ggagactagc
     1681 ggccatgccc agcacgacac cggtcaacat caaacagctc atcgacacct ccgacctctc
     1741 gctcacgcgg gagaacttgg atgatcaggc cctcgaaaaa cgggaggtaa aggacagccg
     1801 cgtacaggtc gtcgggctct ccatcgccgg gttcaccgac catctgtacc gcgaacggat
     1861 ccaactgctc gggaaaagcg agaacgacta tctgaacacg ctcggtgacg cggaacggtc
     1921 ggcgcggata gaatcgttcc tcgcctgcca cccggtcgcc gtcatcttca ccgacccggt
     1981 gcgcccctcc cccgaattca acgcgcgagc cgacgcccac gaggtcccgc tcctctcgac
     2041 ccgcatggtc tcctccattt tcatagattt catcagggag cacctcaacc ggctgctcgc
     2101 cgcctccgcc accctgcacg cccaactcct cgatgtgttc ggcgtgggga tgctcatcat
     2161 gggtgagagc ggcgtcggca aatcggagac atcgctcgac ctcgtgatgc ggggacacaa
     2221 actcatcgcc gacgatgtgg tcgagatacg cttcatcccg cccgaccggc tcgtgggcgc
     2281 ctgcaacgag atgatgcgcg acctcatcga gatacgcggc atcgggctca tcaatgtcca
     2341 gcgcatgttc ggcgttacct cggtcaacta tgagaaacag atagattgca tcatcaatct
     2401 ggtcctctgg gatcagtcgg ccaccttcga atacgagcgg atcggtgcga cgacccgcca
     2461 ctacgagata ctgggggtca agcgccccta ctacatcatc ccggtccggc acgggagcaa
     2521 cctgagttcg ctcatggagg tcatcgcacg cgatcacatg ctcaggtccc aaggcgtcaa
     2581 tgtggccgcg gaattcgaac agaatcttgc gaagaaactg agtgaaatgg gaggtgcatg
     2641 atgttctctt tcatcttcgt gacccacggc gatctcggcg catcgctcgt ccgcaccgcc
     2701 gagttcatca tgaacgagcg gttcgatgac cggtgcgaga ccttctcgct cgattattcc
     2761 atgctcgccg acatggaccg catcaaggaa ctgcttgaac ggtcggtcga tcggatcgtc
     2821 accaaaggtc accgcgccat cgtgttcgtg gacatattcg gcggtagccc gtccaatgtc
     2881 gcgttcactt tcgccaaacg ggacgaggtc gacatcctct ccggcgtcaa cctgccgatg
     2941 gtcatctacg cattcgaaca ccgcgatgcc ccggtgtcga tagcggaact gaccgccggc
     3001 atactccaaa gcggcgacga aagcctcatc agcgcgaaga aacttctgga aaaggagcac
     3061 cgatgaccac atgagggaac acaccgtcac cctgcagtac cgcaaaggca tccacgctcg
     3121 cgccgcctcc ctgttcgcca aggaggcgga acggttctcc tccaaggtcg aactcgaaaa
     3181 gaacggggtg cgcataaacg ccaagagcat caccgggata ctcatgctga agataccgtt
     3241 cggcgccacc ttcaagatca tagtgaacgg ccccgatgag gacgaatgcc tcaccacgct
     3301 cgtccaactg gccgagtcgc gattcgagga ggaactgtga tggcgccgga aaaaagaccg
     3361 gtactcctgc caggcgagaa gatcgtcgaa ggcatcgcca taggcaaggt ctttttcatc
     3421 gacaaggatt tctcgctcat cccgcacctg accctgcgcg gcggacagaa ggcgcgcgag
     3481 aaggaacgac ggcggcttac ggccgcggtc aagaaatgcg agaaggaact cgtttccatt
     3541 atggaggagc gtcacctgcc caaggaggcg acctcgatac tggaggcgca ccgcatgatg
     3601 ctggttgacc ccgaactcct gcgcaagata ggcgagacca tcatggatcg cgcggtcaac
     3661 gccgaatggg cggtactgct tgtgttcgac gagatgatgg cgcacctctc ctcaagcgat
     3721 ggcgattatt accttcgcgc caaggtggct gatcttgagg tcatccgcga caaactcctg
     3781 ttctcgctgc tcggcggcgc cggacgcacc agttacatca aggacctccc caccgaggat
     3841 ttcgttctgt gcgcccatag tctgacgctc tccgacctca gttcgctcgc caaggatccg
     3901 cacctccgcg gcatcgcgct cgaaacgccc ggcggcgtct cccatctcac catcgtgctc
     3961 cgatcgctcg gcctcccgac ggtgctcggc gccgacggac tcctcaccga ggtggtcggt
     4021 tgtgacgagg cggtggtcga cgggctggcc ggacgggtcg tcctgtcacc ctcgccggcc
     4081 gacaagagcg cctatctcaa gaaaaaacag gcctacgacg actatttcga ccggttcctg
     4141 cacaatgccg gcagaccggc gctctcgcgc gatgggtcga tgctccacat cggtgccaac
     4201 atcgaaaatg tcgaggatgt cgagccgtca cgccgctgtg gcgccgactt catcggtctg
     4261 ttccgcaccg aactcatgtt cctcgaaaag ggcgacatac cgaccgagga cgaccattat
     4321 gcggtctatt acgagatgct ccaccgcgct catccgatgg aagtgaccat ccgtgtcttc
     4381 gatttcggcg gggacaagga gggcggcatc cacgcgaccg gcgcgatggg cctgcgcggc
     4441 atacgattct gtttcctgta tcccgatgtg ttcctgccgc aggtgcgcgg ccttatccgc
     4501 gccgcctcgc tcggcaacct gcgcatactc ctccccttcg tttcggcggt cgatgagatc
     4561 cacgagttca aggcgatact gaaggagcag gcgaaggaac tcaaactgga aaagaacctc
     4621 aggcacataa agataggtgc gatgatagaa ctgccggcgg cgctcttcat cgccgagatg
     4681 ctctcccgcg aggtcgattt cttcagcatc gggaccaacg atctcgtgca gtacctcatg
     4741 gcggtcgagc ggcaggacaa ggcgctttcg cagtatttct cccatttcca cccggcggtc
     4801 ctgcgggcgc tctacaacct gcaccacatc gcgaagaaag cccgcataga cctttcggtc
     4861 tgcggcgaga tgggcggcga cccttatttc acgctcctgt tcatgggtat gggtatccac
     4921 gcgctcagca tgacgccgct cgccatcccg atggtcaaga atatcatccg ctccagttcg
     4981 gtcgaggagg ggcgcgcgct cctcaaccgg ctcctcatga ccgcgagcga gatggaactg
     5041 cgcgagacgt tggaaaaaga gatggaacag cggtatcccg atattttccg caaggaatgg
     5101 ctcgataacc ttaagaagaa tggaggctga aatggcgctc acgaaactcg acaccctgct
     5161 tgaaatggcc cgcggcaaga agacatcccg cctcgtggtg gcggcggccg aggatgaacc
     5221 ggtgcttgaa gcggtccgcg acgcgatgaa ggagaagatc atcgttccca tcctcgtggg
     5281 cgatgcgccg aagatcaagg agatcgcggc caaggtcaaa ctgtcgctcg acggcatcga
     5341 gatcatcgac gagaagagcc ccgcgaagtc gagcaagata gcggtccagt gcatccgcga
     5401 agggaaaggc gacatcatga tgaaggggct cgtcgggacg gccgacctgc tgcgcgcggt
     5461 actcgacaag gagaacggcc tgcgcaaggg cgagatcatc agtcatgtcg cgcttcacga
     5521 ggtgaagggc tatcataaac tgctcatggt caccgatgcg gcgctcaccc ccgcccctac
     5581 cttcgcccag aaggtcgata tgatccataa cgccgccgag gtgtaccata aactcggcat
     5641 caaggagccc aaggtcgcgg tcctctgcgc cgccgagacg gtgagcgaga agatcgaggc
     5701 gtcggtccac gcggcgatgc tcacgacgat gaaccggcgc ggccagataa agggggccat
     5761 cgtcgacggc ccgctcgcgc tcgacaacgc cgtatcgaag gaggcctgcg aacacaaaaa
     5821 gatcaagagc gaggtggggg gcgacgccga catactcgtc tgccccgaca tcgaggcggc
     5881 gaacatcctc tacaaggcgc tcaacttcct tgccggcgcg acgacggcag gcgtgctgat
     5941 gggcgcgaag gtgccgatcg tgctgacctc gcgcgccgat accgaccgga gcaaactggc
     6001 ttcgatagcg cttgccgccg ccatggaact ttaaccgcag ggaggaagat atgcgcatcc
     6061 tcgccatcaa ccccgggtcg acctcgacaa agatcgcggt cttcgaggag aacaaggaga
     6121 tcttcaccaa gaatctcaag cacaccgtcg aggaactggc cccatacaag aagatcatcg
     6181 atcagtacga cttccggcgc gatgtcatcg tgaaagaact cgaggcagcc aagatcccgc
     6241 tttcaagcat cacggcggtc gtcggtcgcg gcgggctggt caaacccatc cccggcggcg
     6301 tctaccgcgt gagcgatgcg ctcatcgccg acctcaaggc ggagcggatg ggcgaacacg
     6361 cttcgaacct cggcggtctc atcgcccgcg agatggcgcg caagatcggc cccaaggtcg
     6421 attctttcat cgtcgacccg gtggtggtcg acgagatgga ggatatcgcc cgtatcagcg
     6481 gcatccctga actgccgcgc gtcagcatct tccacgcgct caaccagaaa gcggtcgccc
     6541 ggcgctatgc ccgtgaacag gggaagagat acgaggaact caacctcgtc gtcgtccaca
     6601 tgggcggtgg tatatcggtc ggctgtcata aaaaggggcg tgtcgtcgat gtcaacaacg
     6661 cgcttgacgg cgacggtccc ttcagccccg agcggagcgg cggtgtgccc gtcggccaga
     6721 tggtgaaact ctgcttctcg ggcaagtaca ccatcgacga catcaagaaa aagataaaag
     6781 gcaaaggcgg actggtcgcc tatctcggca ccaacgacgc ctacgaggtc gaacagcgcg
     6841 ttcagaaggg cgacaaggag gcggagcatg tctaccgcgc catggcctac cagatagcca
     6901 aggaggtcgg cgccctttcg accgttctgt gcgggaagat cgatgcggtc attctcaccg
     6961 ggggggtcgc ctacgacaag atattcgtcg gctggctcac cgagcgcatc ggtttcctcg
     7021 cgccggtggt cgtctacccc ggcgaaggcg agatgacggc gctcgctgag gcgggctact
     7081 acgcgatgaa gggtgaacta ccgatacagg actatgtctg aacgtgttcc tctgaatagg
     7141 cgccgtccga gcggcctgcc gctcgcggcc ctcatgctcc tgcttgcgat cgggtgcacc
     7201 acggcccccg ttgatatcga cggaccgtca cctgtgtccc ccgcggtcgc cgccacgccg
     7261 ctttctcgcg tggaaacgcc tacggaggga acactgctac gggggagcga cccccggatg
     7321 ctcatgttcc gcgccttcct cgatcgggcc ggcatccccg tccttttcgg cgtggatggt
     7381 gccggacttt ttgtgctctc ggtggcacgc gatccgaccg atgtcttcc
//
LOCUS       DAOQUE010000077         7427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_10983_length_7427_cov_4.157081, whole genome shotgun sequence.
ACCESSION   DAOQUE010000077 DAOQUE010000000
VERSION     DAOQUE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10983_length_7427_cov_4.157081"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2201)
                     /locus_tag="PKH10_03605"
     CDS             complement(<1..2201)
                     /locus_tag="PKH10_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27245.1"
                     /translation="MGKIIIEGDNSLENMEIRLDHLLFGEKEMEQEKNDFLSFEGLLD
                     ERYRDFPAYLISIGQLAKASALIKGQDEVEIFFLMILAGDEKTLVLRTLEKNREALGE
                     VFYQEMLCLAFLSADTEERKDIMASILQVPQILRTPLATLIVARFSDEPQSIAPGGSL
                     EQEAAVFLEALKAEQTDNIEKAFSDLLALFIRANHPRFLFEYLRSMVLRYDTIAPGSV
                     RQFVETVLRSPLAVSFASMKLLEFLYYWRNNLLDESEETVTQLAEATDSLFVLNAIAP
                     LLYKYGKWHLLGKFYKLVSRKTTGKTRIRYLELLADIYEHKLKMPEFATEIHKNIVEE
                     DPASCSISLSLVLSIYEENRQWDDLANLYRYLAEREKEPRLAAYYLFRGGEILFRELN
                     RPLDARPHLEHSLALNRSFEIIRLLSELYLRLQDYDAYIANLERELEYVGSDAERIAL
                     HERIADALITFKRAYTAAESHLDAILALRPDRIDTVKKLGKIYYTTRNWEKLVEINER
                     EVSLSENTTDIVNLLYKNATIYCNELHDDEKAERSFFQILSIVEHHIPSLLYLEKIYL
                     RRNAPSEIISIYHRLLQSATTDSDTKEFYLTRLAIIYREQGDLRQALDTFRLVEHLYP
                     DSTVARENLRLLSEEVEFREFTLDEFDNGELSSLLAIVNDHGKRGKTPPRWESSTPDL
                     YRYLWEQASLGTAGFPDEQMAPPERFLRQILEGRPTIDLLTKYADRPALLTL"
     gene            complement(2215..3747)
                     /gene="guaA"
                     /locus_tag="PKH10_03610"
     CDS             complement(2215..3747)
                     /gene="guaA"
                     /locus_tag="PKH10_03610"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNT27246.1"
                     /translation="MHRILVLDFGSQYNQLIARRIREMHVYCELHPYSVGIDFIKKFA
                     PDGIIFSGGPSSVTDPGAPDVPKELFDLGIPILGICYGMQLTAHKLGGELGSGHAREY
                     GRAMIEVTAPDSPLLRGVSKRTQVWMSHGDHVAQLPDGFSPIAKTDSIPFGAAGDDTR
                     RIYLVQFHPEVKHTLEGNRILSNFLFEVCRVTADWRMENFIETSVAKIRAAVGDKKVI
                     LGLSGGVDSSVAAALLGKAIGHQLTAIFVDNGLLRLHERDEVEKNFKDVVNLIVVDAT
                     DTFLSRLKGVEEPEKKRKIIGNTFIEVFDTEATKIAGADFLAQGTIYPDVIESSSKMN
                     GPSTTIKTHHNVGGLPDRMKLKLIEPLRDLFKDEVRELGLKLGLPPENVFRHPFPGPG
                     LAVRVLGEVTRERCDILRQADHIYIQEIRSAGLYGDIAQAFAVLLPVKTVGVMGDSRT
                     YEHVLALRAVKTDDFMTADWYDFPHDFLKKVSSRIINEVKRVNRVVYDISSKPPATIE
                     WE"
     gene            complement(3828..5465)
                     /locus_tag="PKH10_03615"
     CDS             complement(3828..5465)
                     /locus_tag="PKH10_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT27247.1"
                     /translation="MKIRSILLRNFLIIQKADLPLDDGLTAVTGETGSGKSLFVEAVK
                     LLLGGKAHKGLTGPWGKTGEIAAIIELEPCDRSLHEDLDTLGIEPEEDRTITLRRVIG
                     EKNILSLNGSPVAAQDLQRLFADRMEISSQFENRELFKPEYQLSVLDATALSDELKET
                     YAARWRDLRAIDEELEDLRRQDDPTRRDYLEFQIREMDEFRPQAGEEDRLKQKMLLLE
                     NRKKIEELLAKSDEGLHATVAAFSPTERALTDLSRIADLGDLPSRASSALIECRDLQR
                     TLAAFSAHMEPDGDDESDLRERHDRLNRLLMKHGVKDAAALIERHNAMKRELAELSRA
                     PERIEELIRRRRKVRDEAEKTALKMRKLRQKAVAPLEKRLTDHLLRFGMDRVVFKLDL
                     APSDDLCPAGLDTLRFLINTIGSGDLYGIKNLSGGELSRLLLALKLVDNDAGRFILFD
                     EIDANIGGEVAARAAEELKKNSRSNQILVVTHFPQTAARAGAHLVVEKSTSATAMESS
                     IRPVEGDERVRELARMMGDSSSKENLTAARKLLRAAL"
     gene            complement(5499..6743)
                     /locus_tag="PKH10_03620"
     CDS             complement(5499..6743)
                     /locus_tag="PKH10_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNT27248.1"
                     /translation="MNISFLEDLYKRRGGIKLGLGRVVEAFPTVKRREIPVYHVAGTN
                     GKGTAVHAIDHLLRGSGLCTGCFVSPHITDFNERILVDGRRISDATVERIFHELEQEI
                     GIFHELSFFEVTFLLAWRWWEEERVERAVVEVGLGGRLDATSVINWPKTDIITSIGLD
                     HTQILGRTAEEIATEKLGIVHRGDRLLLGPTIPDDLADRMERDALAAGACRVERATFT
                     PPDRFTAPSRASLSPEQRANLFLAHRAVTLTEGCDIAPDFSALSIPGRFQRLHDRIIL
                     DVAHNPPAVEALVRHLDAQHLRPHLLFGAMKDKDIGAMLGLLAPAVRDIRIVGLDEGN
                     DRGASTAEIVDRVPPALRGRLLPGTDAVMTMTTALETLRANGSTLLVTGSFFLLERFI
                     RFLSTPPARAILKDLQSPAAGV"
     gene            complement(6740..>7427)
                     /locus_tag="PKH10_03625"
     CDS             complement(6740..>7427)
                     /locus_tag="PKH10_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT27249.1"
                     /translation="AADAGCELADHSFSHDYRLSLRPQEDIVTDLKRNAFALRENTGM
                     TPRGFRAPGYNTSPAMLAALREMGYRYDASRLPSPFYHTAKQILIWLKRLAGKRSASF
                     VGSFVEGCGPQEPLMLEGGLVELPITTLFPPVGLPLIGTALIAFPAILAHLMVSRALK
                     RDIAHIEMHAIDFAETADGPALESLRGKQPDLAYGIERKIKRTRAAIRRFKDRGFTFE
                     TLSVVADRTA"
BASE COUNT         1291 a         2083 c         2499 g         1554 t
ORIGIN      
        1 agggtgagaa gcgccggtcg gtcggcgtac tttgtgagca ggtctatcgt gggacgacct
       61 tcgagtatct ggcgaaggaa ccgctcgggc ggcgccatct gttcgtcggg gaatccggcg
      121 gtcccgaggg atgcctgttc ccagaggtag cggtagaggt cgggggtcga cgattcccag
      181 cgcggcgggg tcttgcctct tttgccgtgg tcattcacga tggcgagcag cgaggagagc
      241 tcgccattgt cgaactcgtc gagcgtgaac tcgcggaact cgacctcttc tgagagcagg
      301 cgcaggttct cccgcgcgac cgtgctgtcg gggtacaggt gctccacgag ccggaaggtg
      361 tcgagcgcct ggcggaggtc gccttgctcg cgatagatga tggcgagccg tgtcaggtag
      421 aattccttgg tgtcggaatc ggtggtggcc gactgcagga gccggtggta gatggagatt
      481 atctcgctcg gggcgttccg acgcaggtat atcttctcga ggtacagcag gctggggatg
      541 tggtgctcca cgatcgagag tatctggaag aaggagcgct ccgccttctc gtcatcgtgc
      601 agttcgttgc agtagatggt ggcgttcttg tagaggaggt tcacgatgtc ggtcgtgttc
      661 tcggaaagag acacctcgcg ttcgtttatc tcgacgagtt tctcccagtt gcgggtcgtg
      721 tagtatatct tgccgagctt cttgacggtg tcgatgcggt cgggtcggag cgccagtatc
      781 gcgtcgagat ggctctcggc ggcggtgtag gcccgtttga aggtgatgag cgcgtcggcg
      841 atccgttcgt gcagcgctat ccgttccgcg tcgctgccaa catattcgag ttcccgttcg
      901 aggttggcga tgtaggcgtc gtagtcctgg agacgcaggt aaagttccga taggagccgg
      961 atgatctcga acgaccggtt gagtgcgagg ctgtgttcga gatgggggcg ggcgtcgagc
     1021 gggcggttga gttcgcggaa gagtatctcg ccgcctcgga acaggtaata ggcggcgagc
     1081 cgtggctcct tctcgcgctc ggcgaggtag cgatacaggt tggcgagatc gtcccactga
     1141 cggttctcct cgtagatgga gaggacgagc gagagactga tggagcagga ggccggatcc
     1201 tcctcgacga tgttcttgtg tatctcggtg gcgaattcag gcatcttgag tttgtgctcg
     1261 tagatgtcgg cgagcagttc gagataccgg atgcgcgtct tgccggtcgt cttacgggac
     1321 accagtttgt agaacttgcc gagcaggtgc catttgccgt atttgtacag gaggggggcg
     1381 atcgcgttga ggacgaacag tgagtcggtc gcttcggcca gttgcgtcac cgtctcttcg
     1441 ctttcgtcga ggagattgtt gcgccagtaa tagaggaact cgagcaactt catcgaggcg
     1501 aaagagacgg cgagcgggct gcgtagcacc gtttcgacga actgccgcac cgagccgggc
     1561 gctatcgtgt cgtagcgcag gaccatggag cggaggtatt cgaacaggaa acgggggtgg
     1621 ttggcacgga tgaagagcgc gagcaggtcg gagaaggcct tttcgatgtt gtcggtctgt
     1681 tccgccttga gcgcttcgag gaacaccgcc gcctcctgct ccaacgagcc gccgggtgcg
     1741 atgctctgcg gttcgtcgga gaagcgggcg acgatgagcg tggcgagcgg ggtgcggagt
     1801 atctgcggca cctgcagaat gctcgccatg atgtctttgc gttcctcggt gtcggccgac
     1861 aggaaggcga gacacagcat ctcctgataa aacacctcgc cgagcgcctc gcggttcttt
     1921 tcgagcgtcc ggagcacgag ggtcttctcg tcgccggcga gtatcatgag gaagaatatc
     1981 tcgacctcgt cctgtccctt gatgagggcg gatgctttgg cgagttgccc gatggaaatg
     2041 aggtaggcgg ggaagtcgcg gtaccgttcg tcgagcagac cttcgaacga gaggaaatcg
     2101 ttcttctcct gctccatctc cttctcgccg aacaggagat ggtcgagccg tatctccatg
     2161 ttctcgagcg agttgtcgcc ttcgatgatg atcttgccca tgatgccttc tcccttactc
     2221 ccactctatc gtggcgggcg gtttcgaact gatgtcgtag accacccggt tcacccgctt
     2281 tacttcgttg atgatgcgcg acgagacctt tttgaggaag tcgtggggga aatcgtacca
     2341 gtcggccgtc ataaagtcgt cggtcttgac ggcgcggagc gccagcacat gttcgtaggt
     2401 gcggctgtcg cccatgacgc cgacggtctt caccggtagg aggaccgcga aggcctgcgc
     2461 gatatcgccg tagagcccgg cggaccggat ctcctgaatg tagatatggt cggcctgccg
     2521 caggatgtcg caccgctccc tcgtcacctc accgagtacc cgcaccgcga gcccggggcc
     2581 ggggaaggga tggcggaaga cattttcagg cggaagaccg agtttgagcc ccagttcgcg
     2641 cacctcgtcc ttgaacaggt cgcgcagcgg ctcgatgagt ttcagtttca tccggtcggg
     2701 gagtccgccg acattgtggt gcgtctttat ggtggtggag gggccgttca tcttagatga
     2761 cgattcgatg acatcgggat atatcgttcc ctgcgcgagg aagtcggcac cggctatctt
     2821 cgtcgcctcg gtatcgaaga cctcgataaa ggtgttgccg atgatcttgc gtttcttttc
     2881 cggttcctcg acccctttga gccgggaaag gaaggtgtcg gtcgcatcga cgacgattag
     2941 gttaacgaca tccttgaaat tcttctcgac ctcgtcgcgc tcgtgaaggc gcaacaggcc
     3001 gttatcgacg aatatcgccg tcagttgatg gccgatggcc ttgcccagca gagccgccgc
     3061 gaccgaggaa tcgacgccgc ccgacaggcc gagaatgacc tttttatcgc cgaccgccgc
     3121 ccgtatcttc gcgacagagg tctcgatgaa gttctccatt cgccagtcgg ccgtcaccct
     3181 gcagacctcg aacaggaagt tcgacaggat gcggttcccc tcgagagtgt gcttcacctc
     3241 ggggtggaac tggacgaggt atatccgccg cgtgtcgtca ccggcggcgc cgaaggggat
     3301 cgaatcggtc ttggcgatgg gggagaagcc gtcgggcagt tgcgccacat gatcgccgtg
     3361 gctcatccat acctgcgttc tcttcgacac gccgcgcagg agcggcgaat cgggcgcggt
     3421 cacctctatc atggcccggc cgtactcgcg ggcgtgtccg ctccccagtt cgccgcccag
     3481 tttgtgggcc gtgagttgca tcccgtagca gatgccgagt atcgggatgc cgaggtcgaa
     3541 cagttccttc ggcacatcgg gggcgccggg gtcggtgacc gaactggggc cgcccgaaaa
     3601 gatgatgccg tcgggggcga atttcttgat gaagtcgatg ccgaccgaat aggggtgcag
     3661 ttcgcagtag acatgcatct cgcggatgcg acgggcgatg agctggttgt actgtgaacc
     3721 gaaatcgaga acgagtatcc ggtgcatgtt gcctccttgc ggcctgaggg gactctcgcg
     3781 gtcagggtcc tatgctagca cgccctgccg cttttttcaa cattatgtca gagcgcggcg
     3841 cggagaagtt tccgtgccgc cgtcagattc tccttggacg aggaatcgcc catcatacgg
     3901 gcgagttcgc ggacccgttc atcgccctcg acggggcgta tcgatgattc catcgcggta
     3961 gcggaggtcg atttctccac gaccagatgg gcgccggccc gcgccgctgt ctgtgggaaa
     4021 tgggtgacca cgagtatctg gttgctccgg gaattctttt tgagttcctc ggccgcccgc
     4081 gcggcgacct cgccgccgat gttcgcatct atctcgtcga agaggatgaa tcgccccgcg
     4141 tcgttgtcga ccagtttgag tgcgagcagg aggcgcgaaa gttcgccgcc ggagaggttc
     4201 ttgatgccgt acaggtcgcc cgagccgatg gtgttgatga ggaaccggag cgtgtcgagc
     4261 ccggcggggc agagatcgtc gctgggcgcg agatccagtt tgaagacgac acgatccatg
     4321 ccgaaacgga gcaggtggtc ggtcaatcgt ttctcaagtg gcgctaccgc cttctgtcgc
     4381 agtttccgca ttttcagcgc cgtcttttcg gcctcgtcgc ggaccttgcg ccgccgccgg
     4441 atcagctcct cgatgcgctc cggggcgcgg gagagttccg ccagttccct tttcatcgcg
     4501 ttgtgtcgct cgatgagggc ggccgcatct ttcacgccgt gtttcatgag cagacggttc
     4561 agacgatcgt gccgctcccg gaggtcgctc tcatcatccc cgtcgggttc catgtgtgcc
     4621 gaaaaggccg caagggtacg ttgcaggtcg cggcattcga tgagcgccga cgaggcgcgc
     4681 gaggggagat cgccgaggtc ggcgatacgt gacaggtcgg tgagtgcccg ttcggtcggg
     4741 ctgaatgccg ctaccgtcgc gtggagcccc tcgtcgcttt tagcgagaag ttcctctatc
     4801 tttttgcggt tctcaagtaa cagcatcttt tgcttcaggc gatcctcttc gccggcctgc
     4861 ggccggaact cgtccatctc ccgtatctgg aattcgaggt aatcgcgacg cgtcgggtcg
     4921 tcctgccgac gcagatcctc cagctcctca tctatcgcgc gcaggtctct ccatcgggcg
     4981 gcataggtct ctttcagttc atcgctcaag gcggtcgcgt cgaggaccga taattgatat
     5041 tcgggcttga aaagttctcg attctcgaac tgtgacgaga tctccatgcg gtcggcgaag
     5101 agtcgctgga gatcctgcgc cgccacgggg ctgccgttca gagagaggat gtttttttca
     5161 ccgatgacgc ggcggagggt aatggtccgg tcctcttccg gctcgatgcc gagcgtgtcg
     5221 aggtcttcat gcagagaacg atcgcacggc tccaactcga tgatcgccgc gatctcgccg
     5281 gtcttgcccc acgggccggt gagccctttg tgggccttgc caccgagcag gagtttcacc
     5341 gcctcgacga aaagactttt gccgctcccg gtctcgccgg tgacggcggt cagtccgtcg
     5401 tcgaggggaa ggtcggcctt ctgtatgatg aggaagttgc gtaagaggat cgaccgtatc
     5461 ttcaccgcgc gctcctgtgg accgggggac ggcccattct atacaccggc ggcgggcgat
     5521 tgcaaatctt taaggatggc gcgcgcgggg ggtgtggaaa ggaagcggat gaacctttcc
     5581 agcagaaaga aactgccggt gacaagtaac gtcgaaccgt tcgcccgaag agtttcgagc
     5641 gccgtggtca tcgtcatgac ggcgtcggtc ccgggaagca ggcgtccccg cagagcgggc
     5701 ggaacgcggt ccactatttc cgccgtcgat gcaccgcggt cgtttccctc gtcgagtccc
     5761 acgatgcgga tatcgcgcac cgccggagcg agaaggccga gcatcgcgcc gatatccttg
     5821 tctttcatcg cgccgaacag gaggtgggga cggaggtgct gggcgtcgag gtgccgcaca
     5881 agcgcctcga cggcgggggg attgtgggcc acatcgagaa tgatgcggtc gtggagccgc
     5941 tggaaacggc ccgggatgga aagcgccgag aagtcgggag cgatgtcgca gccttcggtc
     6001 agcgtgaccg cgcggtgggc aaggaaaagg ttcgcgcgct gttccggcga gagcgatgcc
     6061 cgcgacggcg ctgtgaaccg gtcgggtggc gtgaaggtgg cgcgttccac ccggcaggca
     6121 cccgccgcga gcgcgtcgcg ctccatacgg tcggcgagat cgtcggggat ggtcggaccg
     6181 agcaggagac ggtcaccccg gtggactatg ccgagtttct cggtcgctat ctcttcggcg
     6241 gtccggccca gtatctgggt atggtcgagt cctatcgagg tgatgatatc ggtcttgggc
     6301 cagttgatga cgctcgtcgc gtcaagccgt ccgccgagcc cgacctcgac cactgcccgt
     6361 tcgacacgct cttcctccca ccagcgccac gcgaggagga aggtcacctc aaaaaaggag
     6421 agttcgtgga atatccctat ctcctgctcg agttcgtgga atatccgctc gaccgtcgcg
     6481 tctgatatcc tccgtccgtc gaccagtatc cgttcattga aatcggtgat gtggggcgaa
     6541 acgaaacaac cggtgcacag gcccgaaccg cgaagaagat ggtcgatggc gtggacggcg
     6601 gtccctttgc cgttggtccc ggcgacatgg tagaccggta tctcgcgccg tttgacggtg
     6661 gggaacgcct cgacgacccg accgagtccc aattttatgc cgccgcgccg cttgtagagg
     6721 tcctcgagga acgatatgtt catgcggtac ggtcggcgac gacgctgagg gtctcaaagg
     6781 tgaagccacg atccttgaat cgtcggatgg cggcccgtgt ccgtttgatc ttccgctcga
     6841 tgccgtaggc gaggtctggt tgtttgccac gcagggactc gagcgccgga ccatcggcgg
     6901 tctcggcgaa gtctatcgca tgcatctcga tgtgagcgat atcccgtttc agggcacggg
     6961 agaccatgag atgggcgagt atcgccggga aggcgatgag cgccgtgccg atgagcggta
     7021 ggccgaccgg cgggaacagg gtggttatcg gcagttcgac gagaccgcct tcgagcatga
     7081 gcggttcctg cgggccgcat ccttcgacga aggacccgac gaaggaggcc gagcgcttcc
     7141 ccgcgagccg tttaagccag atgaggatct gcttggcggt gtggtagaaa ggagaaggga
     7201 gccgcgacgc gtcgtagcgg tagcccatct cgcgcaatgc ggcgagcatc gccggcgagg
     7261 tattgtagcc gggggcgcgg aagccgcgcg gggtcatgcc ggtattctca cggagggcga
     7321 aggcgttccg tttgaggtcg gtcacgatat cctcctgcgg ccggagcgac agacggtaat
     7381 cgtgcgagaa actatggtcg gccagttcgc agccggcgtc ggccgcc
//
LOCUS       DAOQUE010000078         7396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_11039_length_7396_cov_4.096717, whole genome shotgun sequence.
ACCESSION   DAOQUE010000078 DAOQUE010000000
VERSION     DAOQUE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11039_length_7396_cov_4.096717"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /gene="lysS"
                     /locus_tag="PKH10_03630"
     CDS             <1..298
                     /gene="lysS"
                     /locus_tag="PKH10_03630"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942911.1"
                     /note="class II; LysRS2; catalyzes a two-step reaction,
                     first charging a lysine molecule by linking its carboxyl
                     group to the alpha-phosphate of ATP, followed by transfer
                     of the aminoacyl-adenylate to its tRNA; in Methanosarcina
                     barkeri, LysRS2 charges both tRNA molecules for lysine
                     that exist in this organism and in addition can charge the
                     tRNAPyl with lysine in the presence of LysRS1; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNT27250.1"
                     /translation="TPEWVDRFELYIAGREIANAFSEQNDPEAQYEAFARQVEEKKKG
                     NDEAVDMDTDYIRALEYGMPPAGGEGIGIDRLVMLLTDAPSIRDVILFPLLRPE"
     gene            302..1606
                     /locus_tag="PKH10_03635"
     CDS             302..1606
                     /locus_tag="PKH10_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT27251.1"
                     /translation="MRFEFFVGLRYLWNTRRTRFLSLITVISVLGIAIGVAALVTVQS
                     IMDGLQNQMKRSILGSKAHALVSTAEGTVSDWQAAAAKVRAVPGVIGVTPLITTEVIV
                     SAREEVLGGIANGVDGATIGTVTLLPEQMAVEKSDIGCLTAPEQCPALVSRRTGDPGD
                     ALYDDLIGARDLPPVVIGREMARYFSVRPGDVLTVISPVGGGMGPTGPLPLSKNFRIV
                     GTFFTGMYEYDFNYLYLDLSDAQGFFSMEGLVSSVAVKVRDIYDIDRISTALREALGE
                     GYTVRNWKEMNRNIFNALLMEKIVMFLILGFIVLVASFNIVATLLMMVLGKVREVAIL
                     KTMGASRRMIMALFAVDGLVIGTVGTLLGLALGLGACVWLDGLELSFAKDVYYMTELP
                     VDMSAGLFAVVALSALLISFAAALYPSRYAARIRPADGLRYE"
     gene            1617..2918
                     /locus_tag="PKH10_03640"
     CDS             1617..2918
                     /locus_tag="PKH10_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT27252.1"
                     /translation="MKFETLVSIRYFRNTRRSGALSLVTVIAVIGMMLGVAALISVLS
                     VMDGFLSEMKEKILSSTAHAHLHKLVGVFDDHEKTARDIRSLDGVAGVTPSLNGEVMI
                     AANGKIVGSIANGIEIATIGEVVELPRQIVPGQGDLACLADLARCPAFAAARAEKDLL
                     DDDDEPPVPPLPIVIGVDMAEQIGVKPGDIVTVISPLGGGMGATGPVPVSRLFVVVAL
                     FRTGMYDYDSKFAYLSLYDAQDFFHAAGRISYLNIKMRDIYRVKEIGARIMDLVGGFP
                     YGIQDWQDMNKTTFKFLEMQKIVMFIILIAIIIVASFGIISTLVMLVMRKVPEVAILK
                     TMGADDGMIRRIFVLDGLLVGAAGTIAGMLLAVIVCLTLEQIEFPLAKDIYFFNKLPV
                     ALSPVSFGIVALCALIISFVATLYPAWKASRIPPAEGLRYE"
     gene            2928..3593
                     /locus_tag="PKH10_03645"
     CDS             2928..3593
                     /locus_tag="PKH10_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT27253.1"
                     /translation="MLELRNITKSYTIEGKTIPVLREVNLELAAGAQMSLIGRSGAGK
                     STLLSIIATIEQPDGGILRIDGKDTTAMPDTLLSRFRNTMIGTVFQFHHLLPEFTALE
                     NVAMPLLIGRRHQEAFGEARALLERVGLKDRAEHKPAEMSGGEQQRVSIARALVGRPR
                     ILLLDEPTGNLDEETGLAILDLVFSLAQEHGLTTIFVTHNKQFARRMATGYELKDGRL
                     WSL"
     gene            3581..5905
                     /gene="bamA"
                     /locus_tag="PKH10_03650"
     CDS             3581..5905
                     /gene="bamA"
                     /locus_tag="PKH10_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNT27254.1"
                     /translation="MVAVRASLLAFLVLVAGLAGAAEIKEVKAEGCARTECSLITKHF
                     EGKTALTEGEIDAILRSLYASKLYQHIEMRYELDTGALRVVVVEQPVITAVSVKGNSK
                     LDTDDIVKVIDIKRNQLVDADKIRGNEQKITELYKEKGYLMVRVAPDLTINEKSNEAT
                     LVFSIAEGERSRVRKITIAGNTFLTDEFIKERLNTQEEGLFSFFKDEGYKKELLEEDQ
                     ARIMYLYNEEGFVNVRVSRPLVAISPDRRDIDITFVVTEGDRYKINSVKMDGDILENG
                     EYPRYEPRLVKDQWYRHTELIADIEALKTFYGDEGFPFVTVLPDRAPIEPDKTLDVTY
                     RIQKGNKCNVERIEITGNDRSRDKVVRREMRLYEGELYTYTGQKRSELLIKRTGFYDE
                     VTITLKEGSQPDLVRVIVKVKERKSGTFNVGAGFSSFESFVFNARIDQNNFLGYGQTL
                     SLVSQFSAMRQEVNLSLFEPYFFDYDVFLNTSFYFVKFDYSSLNSSYYADYEDNRLGY
                     SLIFGYPLTDNLSISGGYRIDKVWLKGGAKNQMNFLFRDMLTSSLLARLTWDSRDDRM
                     FPTKGFYSSFSTEMGPRFLGSDDDILTLDFNFRWYQRLFWNLVYKVNLLTSWKKDFSG
                     NRLPVSERYRLGGIFSIRGYSYNSIGPSEELRVAESPSDPTSSLFPYTIGGDKQIIIN
                     NELEVPILKEMRLNLVAFLDVGNTFNEDENLFYIDNPGRNKYHLPLAMFWSAGFGIRW
                     VTPIAPLSFEWGFPITPRPGDPVYMFEFNIKNSF"
     gene            5916..7238
                     /locus_tag="PKH10_03655"
     CDS             5916..7238
                     /locus_tag="PKH10_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT27255.1"
                     /translation="MREKGTLILGAGLAGISTAYHLGDRDHLVIEKSDHPGGLCTSEH
                     TGGFTFDQTGHWLHLAREENRTLFSDLFPDDRVRIERKTHIFSHGVYTLYPFQSNTYG
                     LPPQVVRECVAGFVKALRDTDKSRARENFYEWCMAYLGEGIAKHFMIPYNSKLYTVHP
                     KQMASHWCDRYVPKPTLAEVIEGAVTAPDRKVGYNASFFYPAQGGIGEFPKRFFARTD
                     AARYLFNARPTALDIENRIVTLDGGEQVRYRHLVSSIPLRDLLNIVTGPFAPTAKEIA
                     ARMQVASVSYLNIALKKPAGHPGHWFYIPEEGFLPYRVGSFSNIHPPLAPAGRGSLYV
                     EYTHQGPFSGVDELMEESIRLLTAMGIIADDEEDIDFIDHRCIENGYVIFHREYFDDM
                     AAVSDIFGRHGVHLVGRYGRWTYNAMEDAVSDGRETAKKILSGQGGPA"
BASE COUNT         1458 a         2273 c         2252 g         1413 t
ORIGIN      
        1 gacgcccgaa tgggtcgacc gcttcgaact gtacatcgcg gggcgcgaga tagcgaacgc
       61 cttctcggag cagaacgacc ctgaggcgca gtacgaggcc ttcgcgaggc aggtcgaaga
      121 gaagaagaag ggtaacgacg aggcggtcga catggacacc gactatatcc gcgctctcga
      181 atacggcatg ccgccggcgg gcggcgaggg tatcggcatc gaccgtctgg tgatgctgct
      241 caccgacgct ccctccatcc gcgatgtcat cctgttcccg ctcctgcggc cggaatagcc
      301 gatgcggttc gagttcttcg tcgggctccg gtatctctgg aacacgcggc gcacccgttt
      361 cctgtcgctc atcacggtca tatcggtgct cggcatcgcg ataggggtgg cggcgctcgt
      421 gacggtgcag tccatcatgg acggcctgca gaaccagatg aaacgctcga tactcgggtc
      481 gaaggcccat gcgctcgtct ccaccgctga aggaacggtc tccgactggc aggccgccgc
      541 cgcgaaggtg cgcgccgtgc cgggggtgat cggagtgacg ccgctcataa cgaccgaggt
      601 catcgtttcg gcgcgcgagg aggtgctcgg cggcatcgcg aacggggtgg acggtgcgac
      661 gatcggcacg gtgaccctgc tccccgaaca gatggcggtc gaaaagagcg atatcgggtg
      721 cctgaccgcc ccggagcagt gccccgcgct cgtttcccgg cgcaccggcg atccgggcga
      781 cgccctgtac gacgacctca tcggcgcgcg cgacctgccg cccgtggtca tcggtcgcga
      841 gatggcgcgc tatttcagtg tgcgtcccgg cgatgtgctg accgttatca gcccggtcgg
      901 cggcggcatg gggcccaccg gtcctctccc gctgtcaaag aatttccgca tcgtcggcac
      961 cttttttacc gggatgtacg aatacgattt caactacctg taccttgatc tttccgacgc
     1021 gcaggggttc ttctcgatgg aggggctcgt gagttcggtg gcggtcaagg tgcgcgacat
     1081 ctacgatatc gaccggatct cgacggcgct ccgcgaggcg ctcggcgagg gttacacggt
     1141 gaggaactgg aaggagatga accgcaatat cttcaacgcg ctcctcatgg aaaagatcgt
     1201 gatgttcctc atactcggct tcatcgtgct cgtcgcgtcg ttcaacatcg tcgcgacgct
     1261 cctgatgatg gtgctcggca aggtgcgcga ggtcgcgata ctcaagacga tgggggcgag
     1321 ccgccgcatg atcatggcgc tgttcgcggt ggacgggctc gtcatcggca cggtcggcac
     1381 gctgctcggg cttgcgctcg ggctcggcgc ctgcgtctgg ctcgacggac tagagctctc
     1441 gttcgccaag gatgtgtact acatgaccga actcccggtc gacatgtcgg cgggactctt
     1501 cgcggtcgtc gctctttcgg cgctcctcat ctctttcgcc gcggccctgt atccgagccg
     1561 ctacgccgcc cgtatcagac cggccgacgg tctgcggtac gagtaggggg gaacgcatga
     1621 agttcgagac actcgtgagt atccgttatt tccggaacac tcgccggtcg ggagcgctgt
     1681 cgctcgtgac ggtcatcgcg gtcatcggga tgatgctcgg ggtcgccgcg ctcatatcgg
     1741 tcctttcggt gatggacggt tttctttccg agatgaagga gaagatactc tcctcgacag
     1801 cgcatgccca cctgcacaaa ttggtcggcg ttttcgacga tcacgaaaag acggcgcgcg
     1861 atatccggtc gctcgacggc gtcgcggggg tcaccccgtc gctcaatggt gaggtgatga
     1921 tagcggcgaa cggcaagata gtcggctcca tcgcgaacgg catcgagatc gcaacgatcg
     1981 gcgaggtggt cgaactgccg cgccagatcg tgccgggaca gggcgatctc gcctgtctcg
     2041 ccgaccttgc gcggtgcccg gccttcgcgg cggcgcgggc cgagaaggac ctgctcgacg
     2101 acgatgacga accgccggtg ccgccgctcc cgatcgtcat cggcgtggat atggccgagc
     2161 agatcggggt gaagccgggc gatatcgtca ccgtcatcag cccgctcggc ggtggtatgg
     2221 gggcgaccgg tccggtgccg gtgagccgcc tgttcgtggt ggtcgcgctg tttcgcaccg
     2281 gcatgtatga ttacgactcg aaattcgcct atctttcgct ctacgacgcg caggatttct
     2341 tccacgccgc cggtcgcatc agttacctca acatcaagat gagggacatc taccgggtca
     2401 aggagatcgg tgcgcggatc atggatctcg tgggcgggtt cccctacggc atacaggact
     2461 ggcaggacat gaacaagacc acattcaaat tcctcgagat gcagaagatc gtcatgttca
     2521 ttatccttat cgccatcatc atcgtcgcct cgttcggcat catctctaca ctcgtcatgc
     2581 tcgttatgcg caaggtgcct gaggtggcga tactcaagac gatgggggcc gacgacggga
     2641 tgatacggcg catcttcgtc ctcgacggtc tcctggtggg cgccgccggg acgatcgcgg
     2701 gcatgctgct cgcggtcatc gtctgtctga cgctcgaaca gatagagttc ccgctcgcca
     2761 aggacatcta tttcttcaat aaactgccgg tcgcgctgtc gcccgtcagt ttcggcatag
     2821 tggcgctctg cgcccttatc atcagtttcg tcgcgacgct ctacccggcg tggaaagcgt
     2881 cgcgcatccc gcccgccgaa gggctgcggt acgagtgagg tgacaggatg ctcgaactga
     2941 ggaacatcac gaaaagttac accatcgagg ggaagaccat ccctgtgctc cgcgaggtga
     3001 acctcgaact ggccgcgggg gcgcagatgt cgctcatcgg caggagcggt gccggaaaat
     3061 cgacactgct ctccatcatc gccacgatag aacagcccga cggcggtatc ctgcgcatag
     3121 acggcaagga tacgaccgcg atgcccgaca ctctgctcag ccggttccgc aacacgatga
     3181 tcggcaccgt gttccagttc caccacctcc ttcccgaatt cactgcgctc gagaatgtcg
     3241 ccatgccgtt gcttatcggt cgccgccatc aggaggcgtt cggagaggcg cgcgcgcttc
     3301 tggagagggt cgggctgaag gaccgcgccg aacacaagcc ggccgagatg tcgggaggcg
     3361 aacagcagcg cgtgtccatc gcgcgggcgc tcgtcggccg gcccaggata ctgctcctcg
     3421 acgaaccgac cggtaatctt gacgaagaga ccggactggc tatcctcgac ctcgtgttct
     3481 cgctggcgca ggagcacgga ctgacgacga tattcgtgac ccacaacaaa cagttcgcgc
     3541 ggcgcatggc gaccggttac gaactgaagg atggtagact atggtcgctg tgagggcatc
     3601 gcttctcgcc tttcttgttc ttgtcgcggg gctcgccggc gcggccgaga tcaaagaggt
     3661 caaggccgag gggtgcgcgc ggaccgaatg ttcgctcatc accaaacact tcgaaggcaa
     3721 gaccgctttg acggaagggg agatcgacgc catactccgg tcgctctatg cgtcgaaact
     3781 gtaccagcac atcgagatgc gctacgaact cgacaccggt gccctgcggg tcgtcgtcgt
     3841 ggagcagccg gtcatcaccg cggtgtcggt gaaaggcaac agcaaactcg acaccgacga
     3901 tatcgtcaag gtgatagaca tcaagaggaa ccagttggtg gatgccgaca agatacgagg
     3961 caacgaacag aagataaccg aactctacaa ggagaagggt tatctcatgg tgcgggtcgc
     4021 ccccgacctg accatcaacg agaaaagcaa tgaggcgacg ctcgtgttct ccatcgcgga
     4081 aggggagcgg agccgcgtcc gcaagataac gatcgccggc aacaccttcc tgaccgacga
     4141 attcatcaag gagcggctca acacgcagga ggagggcttg ttctcgttct tcaaggacga
     4201 aggatacaag aaggaactcc tcgaggagga tcaggcccgc atcatgtacc tctacaacga
     4261 ggagggcttc gtcaatgtcc gcgtttcgcg gccgctcgtc gcgatatcgc ccgaccgccg
     4321 cgatatcgac atcacctttg tggtgaccga aggcgaccgc tataagatca actcggtcaa
     4381 gatggacggc gacatcctgg agaacggtga atacccgaga tacgaaccgc gccttgtgaa
     4441 ggaccagtgg taccgccaca ccgaactcat cgccgacatc gaggcgctca agaccttcta
     4501 cggcgacgag gggttcccgt tcgtgacggt gctgcccgac cgcgccccga tagaacccga
     4561 caagacgctc gatgtcacct accgcatcca gaagggcaac aaatgcaatg tcgaacgcat
     4621 cgagataacg ggcaacgacc gctcgcgcga caaggtcgtc aggcgcgaga tgcgtctgta
     4681 cgagggggaa ctttacacct acacggggca gaaacggagc gaattgctca tcaaacgcac
     4741 cggcttttac gacgaggtca ccataacgct caaggagggc tcgcagccag atctcgtgcg
     4801 ggtcatcgtc aaggtcaagg agcgcaagag cggcaccttc aatgtcggcg ccggcttcag
     4861 ttcgttcgaa tcgttcgtgt tcaatgcgcg gatagatcag aacaacttcc tcggctacgg
     4921 gcagacgctc agtctcgtgt cgcagttctc ggcgatgcgg caggaggtga atctctccct
     4981 gttcgagccc tatttcttcg actacgatgt gttcctgaac accagtttct acttcgtcaa
     5041 gttcgattac tcctccctta attcctccta ctacgccgac tacgaggaca accggctcgg
     5101 ctacagtctc atcttcggct atccgctgac cgacaacctt tcgatatcgg gcgggtaccg
     5161 catcgacaag gtgtggctca aggggggtgc caagaaccag atgaacttcc tgttccgcga
     5221 catgctcacc agttcactcc tcgcccggct gacctgggat tcgcgcgacg accggatgtt
     5281 cccgaccaag gggttctatt ccagtttcag caccgagatg gggccgcggt tcctcggctc
     5341 cgacgacgac atactgacgc tcgacttcaa tttccgctgg taccagcgcc tcttctggaa
     5401 cctcgtctac aaggtcaatc tcctcaccag ttggaaaaag gacttctcgg gcaaccgcct
     5461 gccggtctcg gagcggtatc ggctcggcgg catattctcg atacgcggtt attcctacaa
     5521 ctccatcggc cccagcgagg agttgcgcgt cgccgaatcg ccgtcggacc cgacgagcag
     5581 cctgttcccc tataccatag gcggcgacaa gcagatcatc atcaacaacg aacttgaggt
     5641 gccgatactc aaggagatgc ggctcaatct cgtcgcgttc ctcgatgtcg gcaacacctt
     5701 caacgaggac gagaacctgt tctacatcga caacccgggg cgcaataaat accacctgcc
     5761 gctcgcgatg ttctggagcg ccgggttcgg catccgctgg gtcacgccga tcgcgccgct
     5821 ctcgttcgaa tggggcttcc ccatcacgcc gcggccgggc gacccggtct atatgttcga
     5881 gttcaatatc aagaactcct tctagcggga aggtcatgcg ggagaagggg acgctcatcc
     5941 tcggtgcggg gcttgcgggc atctcgaccg cataccatct cggtgaccgc gaccatctcg
     6001 tcatcgaaaa gagcgaccat cccggcgggc tgtgcacctc ggaacatacc ggcggcttca
     6061 ccttcgatca gaccggccac tggctccacc tcgcgcgcga ggagaaccgc acccttttca
     6121 gcgacctgtt ccccgacgat cgcgtccgca tcgagcggaa gacccatata ttctcgcacg
     6181 gcgtctacac gctctacccg ttccaatcga acacctacgg gttgccgccg caggtcgtcc
     6241 gggagtgtgt cgccggcttc gtgaaagccc tgcgcgacac cgacaagagc agggcgcggg
     6301 agaattttta cgagtggtgc atggcctatc tcggcgaggg catagcgaag catttcatga
     6361 taccctacaa ttcgaaactc tacaccgtcc acccgaaaca gatggcgtcg cactggtgcg
     6421 accgctatgt cccgaagccg acgctcgccg aggtcataga gggggcggtc accgcgcccg
     6481 accggaaggt gggctacaat gcgtcattct tctatccggc gcaggggggc atcggcgaat
     6541 tcccgaagcg cttcttcgcc cgcaccgacg ccgcgaggta tctcttcaac gcccggccga
     6601 cggcgctgga tatcgagaat cgcatcgtca cccttgatgg cggcgaacag gtacgctatc
     6661 gtcatctggt cagttcgata cccctgcgcg atctgctgaa catcgtgacc gggccctttg
     6721 cgccgaccgc gaaagagatc gcggcgcgga tgcaggtggc gtcggtcagt tatctcaata
     6781 tcgccctcaa aaagccggcg ggtcaccccg gtcactggtt ctacatcccc gaagagggct
     6841 tcctgccgta ccgcgtcggt agtttcagca acatccatcc gccgctcgcg ccggcgggac
     6901 gcggctcgct ctatgtcgag tacacccatc agggaccgtt cagcggcgtg gatgaactca
     6961 tggaagagag catccggctc cttaccgcga tgggcatcat cgccgacgac gaggaggaca
     7021 tcgacttcat agaccaccgc tgcatcgaga acgggtatgt gatattccac cgcgagtatt
     7081 tcgacgacat ggcggcggtg tcggacatct tcggtcggca cggcgtccat ctggtcggcc
     7141 gctacggccg gtggacctat aacgcgatgg aggatgccgt gtcggatgga cgcgagacgg
     7201 cgaaaaagat actttctggt cagggaggtc ccgcatgatg cgtatcaccg cgttcctgtt
     7261 cacgctcgga ctgtgcctcg cgctgtgcgc cgaggacccg gaggagccga atttccgtga
     7321 gggtttcgag aagagttgca acgccggcat cgccgaggcg tgcgccctgc tcggcgacat
     7381 gtatttcaaa ggggaa
//
LOCUS       DAOQUE010000079         7381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_11063_length_7381_cov_4.691373, whole genome shotgun sequence.
ACCESSION   DAOQUE010000079 DAOQUE010000000
VERSION     DAOQUE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11063_length_7381_cov_4.691373"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKH10_03660"
     CDS             <1..180
                     /locus_tag="PKH10_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27256.1"
                     /translation="VNDPYHNPDGTLNDCLICDEVNSGPVFKAVAGRNRRNTGLPSSM
                     CRPCAEVQPVFHYYW"
     gene            complement(189..3194)
                     /locus_tag="PKH10_03665"
     CDS             complement(189..3194)
                     /locus_tag="PKH10_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27257.1"
                     /translation="MERTMLLLGLLLLPFVLAGYEGTPIGGEPYEKWEEGAADYFVMF
                     NSLIDDQITLYGEGENRQADACVLESSFNLNSFHIPNDAIVVQAYLVWMGAVDPAKFA
                     EPTDNAVHLKFARDDLVWEKDIIAGDAAKTLGDTTDPFLFQSVQFTADVTTGCTETEV
                     GTTKTGEVAYFTYRKDVTDFFKKIQDDNLTSDNPLKDGLALFGKYTVSGLDCTEDDQY
                     KCNSTMVSNWSILLVYRSEKVKTKKIYLYPGLAYAQGEATTANVSGFSLPRDPLVRLT
                     AMTAGGDATNSIPELPPEFISLKGEMATSAYVLTNNCNPLVNMIYEVYNSNSSVVGWE
                     GDVMCVSGVVGGSDYFGIDNDTFLLDAEEDINLQEHLRLDGTNFDVRISVNQDAILMN
                     YLVVSVDTKSPAFDIPEEAKINFPYNREKHSCSCRKADDPEDAYCVTESLGYPMFFLI
                     RVQNWGTNVATNVTVIDNLDDRLEYVPLTTEMATRFEDPYYPGDGTDWTMIPDLPING
                     SDLNTRFPLSGAGYKVADMMAPCDRVTYTCTDTRLLRFKVKPKKLPTNEIIPNIAVIK
                     EEGSDIEYRTNSNNELKLYRGVCLPLETCPEPHRESCGGFYPGPPWQCSENMPCPDGS
                     VCDLTTHTCDPDPDKACTSAKVSYALGINSPDSGDSPIIVPKGTMGLTLGLTLGQFRL
                     QAENCYSTKYYNFVSVRMDIKKDDPQVAVSNLSLVYDKNGNGTVDPDDPVLVTVLTPE
                     PSSVLFAIPEGAARLFTGADIHYFIVTGDLGYNNTEIPAAASMHLSIPDKGSFLFEDA
                     GSPVAVGERVDFAEFQVEPTGNYFIVTKGLNDPPVPAMSELRKDNPILQLRTKAIDAP
                     NKIKRITVNIPVSATEYDTFGHGIRSLSLYGDTNGDGQPDGGPLAKISEFSDEETAVF
                     EGEQMESYLTFNAGEEKFLIINGDLSGLRCEKTRLEIKRGAVTLLDTSDALEGLPVQS
                     KEIPSPEDCGYRPDNDPPSPCCCACSVIW"
     gene            complement(3305..4198)
                     /locus_tag="PKH10_03670"
     CDS             complement(3305..4198)
                     /locus_tag="PKH10_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HNT27258.1"
                     /translation="MGKTGIPAMFDGFTIRRVYDEQKEIWYFSVIDIIRALIQQPDYN
                     AARNYWKVLKHRLAKEGSESVTKCNQLKLTSIDGKKYLTDVADAETLLRLIQSVPSPK
                     AEPIKLWLAKVGYERMQEMADPERSLDRARENWRKHGRSEKWIQQRMMSQETRNKLTD
                     YWKEHEVTKDEEFAILTNIIHQEWSDLSVKEHKALKGLKTQNLRDHMTEAELIFTALA
                     ELSTRQIAESMAATGIKENATAGKKGGRIAKRARLELEQKTGRPVVSGESFLPPGKKI
                     ERLGDHSSKQKKKAKKKPVKK"
     gene            complement(4308..5060)
                     /locus_tag="PKH10_03675"
     CDS             complement(4308..5060)
                     /locus_tag="PKH10_03675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011623778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT27259.1"
                     /translation="MKKIIMLGIMVALFAAGCQKGGAAPKKELSKEDLKTQMDKISYG
                     IGLDMGKNFKKNEMAINTDLFLKGTNDGLAGKDFLMSDEEFRTVMQEFQQDMMKKMQE
                     GNAKKAAGNREKGKKFLDDNKARPGVKVTESGLQYEVIKEGKGAQPKPEDTVTVHYVG
                     TLTDGTEFDSSIARGEPAKFQLNRVIPGWTEGVGLMNVGAKYRFVIPDNLAYGERQMG
                     DKIMPGSVLVFEVELLGIEKGAAAPAPKPVKK"
     gene            complement(5126..5770)
                     /locus_tag="PKH10_03680"
     CDS             complement(5126..5770)
                     /locus_tag="PKH10_03680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNT27260.1"
                     /translation="MQTACQKQIKPFLLWGVMTGAAFAMNLAVLPYRQALFPLLLFLL
                     PFFGAPVAILVGRLAWPASGLREFAWLSTAIVVLFPPAFFLYHFVVGGVVPAAPEASG
                     LLAALRKSAILAVVAALPEEFFFRGWLQQTAFAHLTRRLFLFITARNLLTSALFGVAH
                     AVAFQNPLSAATFFPSLLFGWLTERSGGSVIPSVLLHTVANLVLFLLLACLRTN"
     gene            5815..>7381
                     /locus_tag="PKH10_03685"
     CDS             5815..>7381
                     /locus_tag="PKH10_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27261.1"
                     /translation="MKYRKILVSVFWPTFIAFFVLAITNLVFIDYGAITAADIHKVAV
                     IVVAAILSLISLLVLISSREIYFEWQEYRKQATELPPIIQRLQAEIVTKDTLLAREKE
                     ILLKKHKKIEEAFAAQKRVNDDLERLQHAYENIIELNKTVWFMDSPLGVLELIADEAI
                     KAVEADRAVIIINDEKGAVSFVGTSVVTEQQREPLKLLIGQTAFNRPLISLLESDRLV
                     RLERASVPHPVAAFLACGAGADCFSFAIRFHKDLRGAAFIATRRDSGLIVRNLTLLEM
                     FFSIVSVYLERNELIGREHDFNDILSEQEKMSSLTHLIEGIARNISAPLNAVRLSREA
                     TETTINSLAAKYPDEVLLSRLRKMTERSFLSADAADRIVASIARKAAQDKVERADHLD
                     LNELVRAELTFLEADIEFQSGYERSTDLAENLPLIFGLYRDFSYLIDAHAYLAMHFLK
                     NNEETRRMLFRTRPTADGVFFSIGMSGGVTVDTLQKIFAGADRQHIARVNIPTLNQTI
                     AQIGITRQLSQEKD"
BASE COUNT         1483 a         2171 c         2083 g         1644 t
ORIGIN      
        1 gtcaacgacc cctaccataa ccccgacggg acgctcaacg actgtctcat ctgcgacgag
       61 gtgaattctg gacccgtctt caaggcggtg gcgggacgca accggcgcaa caccgggctc
      121 ccctcatcga tgtgccgtcc ctgcgcggag gtccaaccgg tattccatta ttactggtaa
      181 agggaccgct accagatcac cgaacaagcg caacagcacg gcgatggcgg atcgttatca
      241 ggccgatacc cacaatcctc ggggctgggg atttctttgg attggaccgg aagcccctcc
      301 aacgcatcgc ttgtgtccaa gagagtgacc gcacctcgct tgatctccag tcgtgtcttt
      361 tcacacctca atccgctcag atcaccattg atgataagga acttttcctc ccccgcattg
      421 aaggtaaggt aggactccat ttgctctccc tcaaaaaccg ccgtttcctc gtcactgaac
      481 tcggatattt tggcaagcgg tccgccgtcg ggttgaccgt caccattggt atctccgtat
      541 agagaaagag atcgaatgcc gtgaccaaag gtgtcgtatt cggttgcgga aacggggata
      601 ttcaccgtta tccgctttat tttattcggc gcatcgatcg ccttagtacg caactgtaat
      661 attgggttat ctttgcgcaa ctcgctcatc gccgggaccg gcggatcgtt caaccctttg
      721 gtgacgatga aatagttgcc ggtcggctcg acctggaact cggcaaaatc gacccgttcg
      781 ccgacggcga ccggtgatcc ggcatcctcg aacagaaaac tgcccttgtc cggtatgctg
      841 agatgcatcg aggcagcggc cggtatctcg gtattgttat agccgaggtc gcccgtcacg
      901 atgaagtaat gtatgtcggc gccggtgaac agacgggccg ccccctcggg gatggcgaac
      961 aggacgctgg acggttcggg ggtcaggacc gtgacgagca ccgggtcatc gggatcgaca
     1021 gtaccgttgc catttttgtc gtacaccagc gagagattgc tcaccgccac ctgcggatcg
     1081 tcctttttga tatccatgcg taccgaaaca aaattgtaat actttgtcga gtagcagttc
     1141 tcggcctgca atctgaactg gcccagcgtc aggcctagcg tcaggcccat cgtccccttg
     1201 ggcacgatga tggggctgtc gccactgtcg ggggaattga tgccgagcgc gtaggagacc
     1261 ttggcgctag tgcaggcctt gtctggatcg gggtcgcagg tgtgagtggt caggtcgcag
     1321 accgatccgt cgggacaggg catgttttcc gagcattgcc aaggaggacc aggataaaat
     1381 ccaccgcaac tttctctgtg cggttcggga caggtctcga ggggcagaca aacacctctg
     1441 taaagtttga gttcgttgtt gctgttggtt cggtattcaa tatcgctccc ctcttccttg
     1501 ataacagcga tattcggaat gatctcgttc gtgggcagtt ttttcggttt taccttgaag
     1561 cgcaaaagcc gcgtatcggt gcaggtatag gtcacgcggt cacacggcgc catcatgtcg
     1621 gcgaccttgt acccagcacc cgataaggga aatctggtat tcaggtcgct accattgata
     1681 ggcaggtcgg gtatcatcgt ccaatcggtg ccatcgccgg ggtaataggg atcctcaaat
     1741 ctcgtcgcca tctcggtcgt caagggaaca tattcgagtc ggtcatcgag attgtcgatg
     1801 accgtgacat tggtcgccac attggtgccc cagttctgta cgcggatgag aaagaacatc
     1861 gggtacccga gcgattcggt gacgcagtag gcatcctccg ggtcatccgc cttgcggcag
     1921 gagcaggaat gtttttcgcg gttatacggg aagtttatct tcgcctcctc cgggatatcg
     1981 aacgcgggcg atttcgtatc gaccgacacg acgaggtagt tcatcaggat ggcgtcctga
     2041 ttgaccgata tccggacatc gaagttggtg ccgtcaagtc tcagatgttc ctgcaggttg
     2101 atgtcctcct cggcatcgag caggaaggtg tcgttgtcta tcccgaagta gtcggagccg
     2161 cccaccacgc cgctgacgca catgacatcg ccttcccaac cgacgacgga ggaatttgaa
     2221 ttgtacacct cgtagatcat attaacgagc gggttgcagt tgttggtaag gacataggca
     2281 ctcgtcgcca tttcgccttt gagagagatg aattcgggcg gcagttccgg tatcgagttc
     2341 gtcgcatcgc ctccggcggt cattgcggta aggcggacga gcgggtctct cgggagcgag
     2401 aatcccgaaa cattggcggt tgtcgcctcg ccctgcgcat aggccaatcc tggatagagg
     2461 tatattttct tggtcttgac cttttccgat ctatacacca gcaggattga ccagtttgaa
     2521 accatcgttg aattgcattt gtactggtcg tcctcggtgc aatccagacc gctcaccgtg
     2581 tacttgccga acaacgcgag gccatccttc agtgggttgt ccgacgtgag gttgtcgtcc
     2641 tgtatcttct tgaaaaaatc ggtgacatcc ttgcggtagg taaaataggc gacctcgccg
     2701 gtcttcgtcg ttcccacctc ggtctcggtg catccggtcg tgacatcggc ggtgaactgg
     2761 acgctctgaa acaggaatgg atcggtagtg tcgcccagcg tcttcgccgc atcaccggcg
     2821 atgatgtcct tttcccaaac aagatcatca cgggcgaatt taagatgtac cgcgttatcg
     2881 gtcggctcgg cgaactttgc cggatcaacg gcgcccatcc agacaaggta cgcctgtacc
     2941 acgatggcgt cattggggat atggaaactg ttcaggttga aggaactttc aagaacgcag
     3001 gcatccgcct gacggttctc cccttcacca taaagtgtta tctgatcatc gatgagcgag
     3061 ttgaacatga cgaagtaatc ggcagcgccc tcttcccact tctcgtacgg ctcacctccg
     3121 atgggcgttc cttcgtatcc ggcgagcacg aacggcagga gaagcaatcc cagcaacagc
     3181 atcgtccgct ccatgggccc ctcccgtgtg gttcatcgtc ttttaggaaa aggtatcgcc
     3241 gttcgaaaga attgtcaagc gggaattatt ttcggaaatg cgttgtacgg aaagatacgg
     3301 acgattactt cttgaccggt ttcttcttcg ccttcttctt ttgcttggaa gaatgatcgc
     3361 ccaatcgttc gatctttttt cccggcggca ggaaactttc gcccgatacg accgggcgac
     3421 cggtcttctg ctccagttcg agccgcgccc gtttcgcgat gcggcctccc ttctttccgg
     3481 cagttgcgtt ctctttaatt ccggtcgccg ccatgctttc ggcaatttga cgagtcgaaa
     3541 gttcggcgag ggcggtaaaa atgagttccg cttcggtcat gtggtcgcgc aggttctgcg
     3601 tcttgagccc tttgagcgcc ttgtgctcct tgaccgacag atcgctccac tcctgatgga
     3661 tgatgttggt caggatcgcg aattcctcgt ccttggtgac ctcgtgctct ttccagtaat
     3721 cggtcagttt gttgcgcgtt tcctgactca tcatccgctg ttgtatccac ttttcgctac
     3781 ggccgtgttt gcgccagttc tcgcgggccc ggtcgagcga ccgttcggga tcggccatct
     3841 cctgcatccg ttcatagccg accttcgcga gccacagttt tatcggctcc gccttgggac
     3901 tcggcaccga ctggatgagc cgaagaagcg tctcggcatc ggcgacatcg gtgaggtatt
     3961 ttttaccgtc gatcgaagtc aatttcagtt ggttacattt tgtaaccgac tcgcttccct
     4021 ctttggcgag gcgatgtttt agaaccttcc agtagttccg ggcggcgttg tagtcgggct
     4081 gttggataag cgcccggatg atgtcaatga ccgagaagta ccatatctct ttctgttcat
     4141 cgtacacgcg gcgaatggtg aacccgtcga acatcgccgg tatgccggtc tttcccatgg
     4201 aataccccct tcggtctttt tctccatggg cttctatcgc cgttcgaaag gattgtcaag
     4261 cgggaactat tttcggaaat gcggttggag gagaacgata cggacgctta cttcttgacc
     4321 ggtttcggag cgggggcggc ggcgcctttt tcgatgccaa gcagttcgac ctcgaacacg
     4381 aggaccgagc cgggcatgat cttgtcgccc atctgccgct cgccgtaggc cagattgtcg
     4441 ggtatcacga agcggtactt cgcgccgaca ttcatgaggc ccacgccctc ggtccagccg
     4501 gggatgacgc ggttcaactg gaacttcgcc ggctcaccgc gcgctatcga actatcgaac
     4561 tcggtgccgt cggtcagggt gccgacatag tggaccgtca cggtatcctc gggtttcggc
     4621 tgggccccct tgccctcctt gatgacctcg tactggagac cgctctcggt caccttgacg
     4681 cccgggcggg ccttgttgtc atcgaggaac ttcttgccct tctcgcggtt gcccgccgcc
     4741 tttttcgcgt tcccttcctg catcttcttc atcatgtcct gttggaactc ctgcatcacc
     4801 gtgcggaatt cctcgtcgct catcaggaag tccttgcccg cgagcccgtc gttggtgccc
     4861 ttgaggaaca ggtcggtgtt gatggccatc tcgttcttct tgaaattctt gcccatgtcg
     4921 agcccgatgc cataggagat cttgtccatc tgcgttttga gatcctcttt gctcaattct
     4981 ttcttggggg ccgcgccccc cttctgacac ccagccgcaa acagcgcgac catgatcccc
     5041 agcatgatga tcttcttcat gatatccttc cctaaggaca gttgataacg gagccgacct
     5101 tatcagaaag gttgcggttt ggcaactaat ttgtgcggag gcaggcgagc aggaggaaca
     5161 gcacgagatt ggcgacggta tgcaggagca ctgagggtat caccgacccg ccgctccgct
     5221 cggtgagcca cccgaacagg agcgagggaa agaaggtcgc cgccgagagc gggttctgga
     5281 aggcgacggc gtgggcgacg ccgaacagcg cgctcgtcag caggttccgc gccgtaatga
     5341 aaaggaacag acggcgtgtc aggtgggcga aggcggtctg ttggagccag ccgcggaaga
     5401 agaactcctc aggcagggcc gcgacgaccg cgaggatggc gctcttgcgg agcgccgcga
     5461 gcaggccgga cgcctcaggc gccgccggca cgacaccgcc gacgacgaag tggtagagga
     5521 aaaaggcggg cgggaacagc acgacgatgg ccgtcgaaag ccaggcgaac tcgcgcaggc
     5581 cggatgccgg ccacgcaagg cgcccgacga ggatcgcgac gggagcaccg aagaacggca
     5641 ggaggaaaag caggagcggg aacagcgcct gacggtacgg gaggacggcg aggttcatcg
     5701 cgaacgcggc gccggtcatc accccccaca ggagaaaggg ttttatttgc ttttgacagg
     5761 ccgtttgcat aaactcctct tccgggacga ccgctatagt aaatggtggg cacgatgaag
     5821 tacagaaaaa tcctcgtttc ggtcttctgg ccgacattca tcgccttctt cgtcctcgcg
     5881 ataaccaatc tcgtcttcat cgattacggc gcgataacgg ccgccgacat ccacaaggtc
     5941 gccgtgatcg tcgtcgcggc gatactttcg ctcatatcgc tgctcgtcct catctcgtcg
     6001 cgcgagatat acttcgagtg gcaggaatac cggaaacagg ccacggaact gccgccgatc
     6061 atccagcgcc tgcaggccga gatcgtcacc aaggacacct tgctcgcccg cgagaaggag
     6121 atcctgctca agaaacacaa gaagatagag gaggccttcg ccgcccagaa gcgggtcaac
     6181 gacgacctcg aacggctcca gcacgcctac gagaatatca tcgaactcaa caagacggtc
     6241 tggttcatgg acagcccgct cggcgtgctc gaactcatcg ccgacgaggc gataaaggcg
     6301 gtcgaggcag accgcgccgt catcatcatc aacgacgaaa agggggcggt gtctttcgtc
     6361 ggcacctccg tcgtcaccga gcagcagcgc gaaccgctca aactccttat cggccaaaca
     6421 gcgttcaacc gcccgctcat atcgctcctc gagtccgacc ggctcgtgcg gctcgagcgc
     6481 gcttcggtac cgcacccggt agcggccttc ctcgcctgcg gcgccggcgc cgattgtttc
     6541 agcttcgcga tacggttcca caaagacctg cgcggcgccg ccttcatcgc cacccgccgc
     6601 gattcggggc tcatcgtcag gaacctcacg ctcctcgaga tgttcttctc catcgtctcg
     6661 gtctatctcg aacggaacga actcatcggc cgcgaacacg atttcaacga tatcctgtcg
     6721 gaacaggaga agatgtcctc gctcacccac ctcatcgaag gcatcgcgcg caacatcagc
     6781 gccccgctca atgccgtacg gctgagccgc gaggcgaccg agacgaccat caactcgctc
     6841 gccgccaagt atcccgacga ggtgctcctc tcccgtctgc gcaagatgac cgagcgatcc
     6901 ttcctgtcgg ccgacgccgc cgaccgtatc gtcgccagca tcgcccgcaa ggcggcgcag
     6961 gacaaggtcg agcgggccga ccatctcgac ctcaacgaac tcgtccgcgc cgagctgacc
     7021 ttcctcgagg ccgatatcga attccagagc ggctacgaga gatcgaccga tctggccgag
     7081 aacctgccgc tcatcttcgg gttgtatcgc gatttcagtt acctcatcga tgcccatgcc
     7141 tatctcgcga tgcatttcct caagaataat gaggagacgc ggcgtatgct gttccgcacg
     7201 cgccctaccg cagacggcgt attcttttcc atcggcatgt cgggcggcgt aacggtcgat
     7261 acgctgcaga agatattcgc cggcgcggac cgccagcata tcgcacgggt caacatcccg
     7321 acgctcaacc agacgatcgc gcagatcggt atcacgcgcc agttgtctca ggaaaaagat
     7381 a
//
LOCUS       DAOQUE010000080         7371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_11087_length_7371_cov_5.125478, whole genome shotgun sequence.
ACCESSION   DAOQUE010000080 DAOQUE010000000
VERSION     DAOQUE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11087_length_7371_cov_5.125478"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..706
                     /locus_tag="PKH10_03690"
     CDS             41..706
                     /locus_tag="PKH10_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27262.1"
                     /translation="MAYSTTGGITTPLVKIYTNHSDFQNQQSSWNPADTSWGIHSLMK
                     ETEVQIRTYIFGIGYVWLWYDTDSRTDSFKQFQISIYKNGGGDAMGSSGGAELLFSSD
                     LSAAAAMTEEEESPETIPERSVVTGGFDGIFPAIFRTTEPEDEDDTETTSVKVESNDK
                     GEAVALTVEWVDPAAGEEAKTLLEGMTVVAVNGIDLYDIGAVEEQVLYLVTEDIVSIT
                     FAE"
     gene            complement(661..2733)
                     /locus_tag="PKH10_03695"
     CDS             complement(661..2733)
                     /locus_tag="PKH10_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27263.1"
                     /translation="MKQIFGWFLFGILFSLSGCTGESSQYADTDTSTGDNDLFPSDDE
                     QGEETDDDDPFLYNDDGLRLPLCDPPCNEDEVCNPYGECICDSRKWFVGKYQGKCING
                     GEANKIFCNDHGLNYTARASFWKGGMLVLSKESDLLCALCQPGWFGERCDVPLDDYAD
                     ELPEGGYPNNPGGFRPVKPYCENNADCTEGLICNDKGYCMCEGNISFKDAGFESDVRQ
                     NLGIDAATPITGEMLTNMNSFLTDQAKNLEDLRCMPNLRELYLTMAQNLSSLEPIKGL
                     KHLQGFLLWGMDLSHLDLSPVTELDRLLRFHLAAIYGLPGSDTTFLRKMKSKKSMVHL
                     FLNFGKILFPVDLSFLQDYENLALLQLIFRGDQETMRPTPLSLLPYPEKLQSLGMEGR
                     NIQIDDPTVFLRMANLVSLVILEPGSNIGLSQFSYNEKLINLTMEGDMMDIPVRERFP
                     NTTFVSMRNAVEKLDEKIAGLDMGSLIYSSEFDTKPLFPAGIYAQALSDMNNLNTVYF
                     SPYYDIADIGWLKDKKYLSTVLLQPWCGEDCFPHDWDYPRNNSVYQDLLYLVDCTDYM
                     GGVRSLDDFVTNCKNGGSLCRKDDYLAYIQMPSGDLDMGRVLYFLNNPIDPDTEENNL
                     QFLSKYRVSVELEAEIAKKRVSLRQENFSSDELFHVDTFFSDPWPQSVGKADHSAKVI
                     ETISSVTR"
     gene            complement(2799..3866)
                     /locus_tag="PKH10_03700"
     CDS             complement(2799..3866)
                     /locus_tag="PKH10_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27264.1"
                     /translation="MRNMILFTLLFAFANAFAINDSPVYPCQGYKDLTNIDVYYWWQY
                     GGEGYINSESDFYSSRPSGNSTWCMIQLPDVLDSPQEYGEHWDYGYTYSHVLSLKLYG
                     NYRKSYRAVISPVSSSNLEIIEVFTECAFTYSVRKNAETTDTWALSEWTMTSALSPCS
                     GTFSPGGIFGMYNILDNIDKWVQVLYFWLWVDTEAREDSYKDFVVYIEKWDSGGPDSF
                     MGDQEAVKAMNRMLDDRPIVADIDLDTLKPGEKREYENLDKTIQGILNSTDEFETGTV
                     LSTAITKDGLYAGITIEQVAPQFADEKEVTFFNGKTIISVNGIAVVDFASLEELFKYF
                     SDGEVYEIDITDETGVVTTLH"
     gene            4133..4699
                     /locus_tag="PKH10_03705"
     CDS             4133..4699
                     /locus_tag="PKH10_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT27265.1"
                     /translation="MDIQDKDRNDEKALLERARRSDVAAFETLVRSHEDKLFSFALSI
                     TGGKDEAAYEILQETLIRAYRGIASFRGDCAFPNWLWRILKNNFERYKDREHAWEGKR
                     KGPGDTLEVSTEEQAIHEERLTNIRQLISLLSIDDQETLNLIEFQKLSLEEAAALTGI
                     SIEAFKSRLHRARERLARSVKKNKNLFL"
     gene            4735..5460
                     /locus_tag="PKH10_03710"
     CDS             4735..5460
                     /locus_tag="PKH10_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27266.1"
                     /translation="MDERCRRIDELLTDAMTRELSPEERAILDDHCAACPSCAGQYRA
                     LTAVRETLRRHRPEDDALSEDMRAALHRRLVEESARPERAVGALPFGPRWKLAGAMFA
                     LIMLTLCLFSFLATIPDGADEGTVVSRSVVNAGEPLTIRLTYTADRSLADVRVTVQLD
                     DGVFFHTDDETLRRLKEYVWKGDLKEGRNEIPFVVEVARTGTHRIRTAAAYDGMLHRH
                     EVELQAGPGTVAVIYRSFPERPL"
     gene            5457..5996
                     /locus_tag="PKH10_03715"
     CDS             5457..5996
                     /locus_tag="PKH10_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27267.1"
                     /translation="MRRLGVAALLLVPLFFLYAGKKDTLKSDITVEPGVYKKGWERDF
                     FRDLPPHDPPEESTDAVPVSPDADAMNHRRAVTAPRRMPTPTVTPEPVVEPAPGPATT
                     NPEEKGLDDTPPPPHEERRAPRRPPEPVILPDLPPEEKTPPATGTPAADPEKKRKERL
                     RELENKRKKGNFRDPKITY"
     gene            5974..6975
                     /locus_tag="PKH10_03720"
     CDS             5974..6975
                     /locus_tag="PKH10_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27268.1"
                     /translation="MIRRSLISLLLLCSVARGVETSWEGQVAVGAKYNSNVEYLYLID
                     RLSGDDLPRKEAFFALLNGDVSLVIGEEYTGKIGYSLVSESGLDRPELSRFDQYIAGS
                     FSHLFDDALLLDCSLVLHHAAERWPVPRQLSIDLFGWVTVLYDLNEHFSPYGSARFGY
                     YHDIDPFGDRRLDYFRGPAGGVEGGWYIYPAADTSYLKTGTGLDLNWLRDEEYDRFTD
                     RTGDVLGVSNKHLRITALVEGAFVDERVTAAVALRYVYQYWLGVDMFRYLDWEKRRIE
                     HTIYLQPELEYRFLEHYAVRVTGSVMRKLSNIGEHRDDYTDYSFLQFTGAASFSYTF"
     gene            7013..>7371
                     /locus_tag="PKH10_03725"
     CDS             7013..>7371
                     /locus_tag="PKH10_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27269.1"
                     /translation="MRIEMDAMLSPADAADRDEAATPDGDILVSLVTGGGAGIDRGTA
                     ILDAGDGAIVLGGVTDGELEPGKKKGGIDMFLARYEADRSRAWLRQIGSGGDDTLTAV
                     ARDAEGALYAVGHTYGAL"
BASE COUNT         1703 a         2086 c         2016 g         1566 t
ORIGIN      
        1 ttggggacaa tatgaactga actagcgcat ctggagccag atggcctaca gcacgaccgg
       61 cggcatcacg accccgctcg tgaagatata caccaaccac tccgacttcc agaaccagca
      121 gagcagttgg aaccccgccg acacgagttg gggcatccac tccctcatga aagagaccga
      181 ggtacagata cggacctata tcttcggcat cggttatgtc tggctctggt atgacaccga
      241 cagccgcacc gattcgttca aacagttcca gataagcata tataagaacg gcggcggcga
      301 cgccatgggg tcgagcggcg gcgcggaact gctcttttca tccgatcttt cagccgcggc
      361 ggcgatgacg gaggaggagg agagtcccga gacgatcccg gaacgaagcg tcgtcaccgg
      421 cgggttcgac ggcatcttcc cggcgatatt ccgcaccacc gaaccggagg acgaggatga
      481 caccgaaacg acatcggtca aggtggaatc gaacgataag ggggaggcgg tcgccctcac
      541 cgtcgagtgg gtcgatccgg cggcgggaga ggaggcgaag accctgctgg aagggatgac
      601 cgtcgtcgcg gtgaacggca tcgacctgta cgatatcggc gccgtcgagg aacaggtgct
      661 ctatctggtc accgaggata tcgtctcgat caccttcgcc gagtgatccg cttttcccac
      721 tgactgaggc catggatcgg aaaaaaaggt atcgacatgg aagagttcat cggaggagaa
      781 gttttcctgt cgaagagata ctcttttctt cgcgatctcc gcctccagtt cgacactgac
      841 cctgtacttt gaaaggaatt gcaggttgtt ttcttctgtg tccgggtcta tcgggttgtt
      901 caggaaatag agaactcgcc ccatgtcaag atcgcctgag ggcatttgaa tataagcgag
      961 ataatcgtct tttcggcaaa gagacccgcc atttttacaa ttcgtcacaa aatcatcaag
     1021 cgagcgaact ccgcccatgt aatcggtgca atcgacgaga tacaacaggt cttgataaac
     1081 agagttgttg cgcgggtagt cccagtcatg tggaaaacaa tcttcgccac accacggttg
     1141 caacaatacg gtcgaaagat atttcttgtc tttgagccaa ccgatgtcgg cgatatcgta
     1201 gtaaggagaa aaataaacgg tattgaggtt gttcatgtct gacagtgctt gagcataaat
     1261 gcctgcgggg aacagcggct tcgtgtcaaa ttcggaagaa tatatcaaac tacccatatc
     1321 gagccccgca atcttttcat caagtttttc cacggcattc cgcatcgaaa cgaaagtggt
     1381 attaggaaaa cgctctcgta cggggatatc catcatgtct ccttccatcg tcaaattgat
     1441 gagtttttca ttgtaggaga attgagaaag tccgatgttg ctgcccggtt cgaggataac
     1501 caaggacacg agatttgcca tacgaaggaa aacggttggg tcgtctatct gtatgttcct
     1561 tccttccatg ccaagcgact gtagtttctc gggatacgga agcaaggaaa ggggtgttgg
     1621 tcgcatggtc tcttggtctc ccctgaaaat taactggagc aacgcaaggt tttcgtaatc
     1681 ctgcaaaaaa gaaaggtcca cggggaaaag tattttacca aaattgagaa ataaatgcac
     1741 catgcttttc ttcgatttca ttttgcggag aaaggtcgta tcgcttcccg ggagaccata
     1801 tatggccgcc aagtgaaacc gtaaaagtct atctaattcg gtgaccggtg aaagatccaa
     1861 gtggctcaaa tccattcccc aaagcaaaaa accttgaaga tgctttaatc cttttatcgg
     1921 ttcgagagaa gagagattct gcgccatagt taaatacaat tcacgcaggt tgggcataca
     1981 gcgcagatct tcaaggtttt ttgcttggtc cgttagaaaa gaattcatgt tcgtaagcat
     2041 ttccccggtg atgggcgttg cagcatcaat gccgagattc tgtctcacat ccgactcgaa
     2101 tccggcgtct ttgaacgaga tattcccttc gcacatacag taacccttgt cgttacagat
     2161 caatccctcc gtgcaatcgg cgttgttttc gcagtagggt ttcaccgggc gaaatccgcc
     2221 gggattattg ggatagccac cctccggtaa ttcgtccgca taatcatcca gtggcacatc
     2281 gcaccgttcg ccgaaccaac cgggctgaca aagagcgcag agcagatcgc tctccttact
     2341 caaaacaagc attcctcctt tccagaaaga ggccctcgcg gtatagttca agccatggtc
     2401 attacagaaa atcttgtttg cctctccgcc attgatacat ttaccttgat actttccgac
     2461 aaaccacttc cggctgtcgc agatacattc gccgtaaggg ttacacacct cgtcctcgtt
     2521 gcatggtggg tcgcagagcg gaagacgaag gccatcgtca ttgtagagaa aagggtcgtc
     2581 atcgtcggtt tcttccccct gttcgtcgtc ggaaggaaaa agatcgttgt ctccggtcga
     2641 tgtgtcggta tctgcatact gagacgattc ccccgtacag ccggaaagag aaaacaatat
     2701 cccgaacagg aaccatccga atatctgctt catgccttta cctccgaata gtgaattccg
     2761 gggagaaggg tttatcctcc tccccgtaaa cgaaaaggtt aatgcaatgt tgtgaccacc
     2821 ccggtttcat ctgtgatatc aatctcatat acttcgccat cggaaaaata cttgaacagt
     2881 tcttccagag aagcaaagtc gaccacggcg ataccgttga ccgagatgat ggtcttgccg
     2941 ttgaagaagg ttacctcttt ctcgtcggcg aattgaggcg caacctgttc aatggtgatc
     3001 ccggcataca ggccatcctt ggttatcgcg gtacttaaca ccgttcccgt ctcaaattca
     3061 tcggtgctgt tgaggattcc ctgaatcgtt ttgtcgagat tctcgtactc ccttttttcg
     3121 ccgggtttca aggtatcgag atctatatcg gcaacaatcg gtctatcatc aagcatccga
     3181 ttcatcgcct taaccgcttc ttggtccccc atgaaactat ctgggccgcc tgaatcccat
     3241 ttttcgatgt agacgacaaa atctttatag gagtcttctc gggcttcagt gtcgacccac
     3301 aaccagaaat aaagcacctg tacccacttg tcgatgttgt ccaaaatgtt gtacatacca
     3361 aaaatgccgc cggggctaaa agtccccgaa cagggagaaa gagcactggt catggtccat
     3421 tcactcagcg cccaagtatc cgttgtctca gcattttttc gaaccgaata agtaaatgca
     3481 cactctgtaa aaacctctat gatttccaga ttgctcgaag aaaccggtga aatgaccgcg
     3541 cgatacgatt ttctgtaatt tccatagagc ttgagcgaaa gcacatgcga gtatgtgtat
     3601 ccatagtccc agtgttcgcc atactcttga ggagaatcaa gaacatcggg aagttgtatc
     3661 atgcaccaag tgctgttgcc gctcgggcga gaagagtaaa agtcactttc ggagttgatg
     3721 tacccttcgc cgccgtactg ccaccagtaa tacacatcga tgtttgtgag atccttgtat
     3781 ccctgacaag gataaactgg actgtcgttg attgcgaagg cgttcgcaaa ggcgaacaga
     3841 agcgtgaaaa ggatcatgtt tctcatggtc attctcccat agtgttgttt ttctcttttt
     3901 gtcaagcact cggttttgct tacgatttct gttcggcggc cctcgggatt taaataccgc
     3961 acaacaccgt atgcccattt aacaacatcc tcccttccgc cccataagat acggcatcgg
     4021 aagaaaagga ttcggggtaa aaagaaaaaa cttgaacggg cgcgaagaac cggtattatc
     4081 ggcggacggt acggcagggt gtcgtcatcg gagcgggaaa cgggataacc gattggatat
     4141 tcaagataaa gaccgcaacg atgaaaaggc gctcctcgaa cgggcgcgcc gcagcgatgt
     4201 cgccgccttc gagacgctcg tccgttcgca cgaagacaaa ttgttctcgt tcgccctctc
     4261 catcaccggc ggaaaggacg aggcggcgta cgagatacta caggagacgc tcattcgcgc
     4321 ctatcgcggc atcgcctcct tccggggcga ctgcgcgttc cccaattggc tgtggcggat
     4381 actgaagaac aacttcgaac gctacaagga ccgcgaacat gcgtgggagg ggaagaggaa
     4441 ggggcccggc gacacccttg aggtctccac cgaggaacag gccatccacg aggagcggct
     4501 gaccaacatc cggcaactca tctccctgct gagcatcgac gatcaggaaa cgcttaacct
     4561 catagaattc cagaagttat cgctcgaaga ggcggccgcg ctgaccggca tatcgatcga
     4621 ggccttcaag tcgcgactcc accgggcccg ggagcggctc gcgcggtcgg tgaaaaaaaa
     4681 taagaatctt tttctctaga cgatgtatct tatggggtga ccaaggagaa ggccatggac
     4741 gaacggtgcc gacggattga cgagttactg accgacgcga tgacgcggga actttctccc
     4801 gaagagcgcg cgatcctcga tgaccattgc gccgcctgcc cctcctgcgc cggacagtac
     4861 cgcgccctca ccgcggtgcg cgagaccctg cggcggcacc gccccgagga tgacgccctt
     4921 tccgaggata tgcgcgccgc gctccaccgg cggctggtcg aagagtcggc ccggccggaa
     4981 cgggccgtcg gcgctctgcc gttcggtccc cgctggaaac ttgccggcgc gatgttcgcc
     5041 cttatcatgc tgacgctctg cctgttctcc ttcctcgcga cgatccccga cggggccgac
     5101 gaagggaccg tcgtttcacg ctcggtcgtc aacgccggcg agccgctcac catccggctg
     5161 acctataccg ccgaccggtc gctcgccgat gtccgggtaa cggtccaact ggacgacggc
     5221 gtatttttcc ataccgacga cgaaacgctc cggcgcctga aggaatatgt ctggaaaggc
     5281 gacctgaagg aagggaggaa cgagataccc ttcgtggtgg aggtggcccg gaccggcacc
     5341 caccgcatcc gtaccgccgc cgcgtacgac gggatgctgc accggcacga ggtcgaactg
     5401 caggccgggc cggggaccgt cgccgtcatc taccgttcct tcccggaaag accgttatga
     5461 gacgcctcgg cgtcgcggcg ctcctgctgg tccccctgtt cttcctgtac gcggggaaga
     5521 aagatacgct caagagcgac atcacggtcg aaccgggcgt gtacaaaaaa gggtgggaac
     5581 gggacttttt ccgcgacctt ccgccgcatg acccgccgga agagagcacc gacgccgtgc
     5641 cggtatctcc cgatgccgat gcgatgaacc accggcgcgc cgtaacggcc ccccgcagga
     5701 tgccgacacc gaccgtcacc ccggaaccgg tcgtcgaacc ggcacccggc cccgccacga
     5761 cgaaccccga agaaaagggt ctcgacgata ctccgccccc tccgcatgag gaacggcggg
     5821 cgccgcgccg accgccggaa ccggtcatcc tgcccgacct gcccccggag gaaaagacac
     5881 ctcccgcaac cgggacgccc gccgccgatc cggaaaagaa acggaaagag cggctccggg
     5941 aactggagaa taaacggaaa aaagggaatt tccgtgatcc gaagatcact tattagtctg
     6001 cttctcctct gctcggtcgc gcgcggggtc gagacctcgt gggaagggca ggtggccgtc
     6061 ggcgcgaagt acaacagcaa tgtcgagtat ctctacctca tcgaccgtct ttcgggcgat
     6121 gatctgccgc gcaaagaggc gttcttcgcg ctcctgaacg gcgatgtatc ccttgtcatc
     6181 ggcgaggagt acaccggcaa gatcgggtac tcgctcgtct cggaatcggg actcgaccgg
     6241 cccgaactct cgcggttcga ccagtatatc gccgggagtt tctcccatct tttcgatgac
     6301 gcgctcctgc tcgactgttc gctggtgctc catcacgccg ccgaacggtg gccggtaccg
     6361 cgacaacttt ccatcgacct gttcggctgg gtcaccgtgc tgtacgacct taacgagcat
     6421 ttctccccgt atggctccgc gcggttcggg tactatcacg acatagaccc gttcggcgac
     6481 cggcggctcg actatttccg gggtccggcg gggggtgtcg aaggcgggtg gtacatctac
     6541 ccggcggccg acaccagtta cctcaaaacg gggaccgggc tggacctgaa ctggctgcgc
     6601 gacgaagaat acgatcgctt taccgaccgg acgggcgatg tgttgggggt cagcaacaag
     6661 cacctgcgca tcacggcgct ggtggagggg gccttcgtcg acgaacgcgt caccgcagcg
     6721 gtcgcgctcc ggtatgtcta tcagtactgg ctcggcgtcg acatgttccg ataccttgac
     6781 tgggaaaaac ggcgcatcga acacaccatc tacctgcaac cagaactcga gtaccggttc
     6841 ctcgaacatt acgcggtccg ggtgaccggc agcgtcatgc gaaaactatc caacatcggc
     6901 gagcatagag atgattacac cgattattcg ttccttcagt tcaccggggc ggcctctttc
     6961 tcttacactt tttagtgcga tcgccgccct gtgcatagcc tgcgaagcgc cggtgcgcat
     7021 cgagatggat gcgatgcttt cccccgccga cgccgccgat cgggatgaag cggcgacgcc
     7081 cgacggcgat atcctcgtct ccctcgtcac cggcggcggc gccggcattg accgcggcac
     7141 ggcgatactc gatgcgggtg acggggcgat cgtgttgggc ggcgtcaccg acggggaact
     7201 cgaaccgggt aaaaagaagg gggggatcga tatgttcctc gcccggtacg aggccgaccg
     7261 ttcgcgtgcg tggctccgtc agatcgggag cggcggcgac gacaccctga ccgccgtggc
     7321 gcgcgacgca gaaggggcgc tctacgccgt cggtcatact tacggcgcgc t
//
LOCUS       DAOQUE010000081         7326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_11175_length_7326_cov_4.010728, whole genome shotgun sequence.
ACCESSION   DAOQUE010000081 DAOQUE010000000
VERSION     DAOQUE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11175_length_7326_cov_4.010728"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKH10_03730"
     CDS             complement(<1..285)
                     /locus_tag="PKH10_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT27270.1"
                     /translation="MRIVLQGLERSFTDPSGNRRRVIAGVSADIAPGALVAVTGPSGA
                     GKTTFFNLISGLLLPDSGTITAGGIELAKLSERDRDRWRAKEVGYIFQTFN"
     gene            complement(295..669)
                     /locus_tag="PKH10_03735"
     CDS             complement(295..669)
                     /locus_tag="PKH10_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNT27271.1"
                     /translation="MKVCFPVEENKGLDSIVYNHFGSAPLFVNVDVDTLAVEVLQNSD
                     QHHDHGMCHPLQALAGHEIDIVVLGGIGAGALSKLNAAGIAVYKTGAATVKENVELVK
                     QKRLSLMSGTCGGHGHGGGCGH"
     gene            complement(666..1094)
                     /locus_tag="PKH10_03740"
     CDS             complement(666..1094)
                     /locus_tag="PKH10_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNT27272.1"
                     /translation="MPRPRKCRRVGRPPAFVRFKPQGIPFRSLRKVTLSLDEFEAIRL
                     ADHEGLEHASAAERMNISRPTFTRLIETARRKLAQAIVEGAALVIEGGAVDVEGDFCR
                     CPRCGGVREHRHGGHRERCGYCGTEHECNDTKPITEEERS"
     gene            complement(1094..1846)
                     /locus_tag="PKH10_03745"
     CDS             complement(1094..1846)
                     /locus_tag="PKH10_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNT27273.1"
                     /translation="MTLFLIIVFTVAGSLGSVGVAGLVLLFKERQLANLTGALIPYAI
                     GALLGAAFFGMIPHAMEHLPSDRVLPTVLFGIMGFYLLEKVTLWRHCHEHDHPCPVHT
                     RAGSMILIGDAFHNFVDGVAIGAAFLASIPVGITTAVAVIAHEVPQEVGDFAILLENG
                     YSRPRALAYNLLSSAVSLPGALAAYYLLPLVSSVVPYLLAIAAASFLYIALADLVPGR
                     RVAGGVKSLLWEIPLILTGMLTVVVIGEVIGH"
     gene            complement(1890..2789)
                     /locus_tag="PKH10_03750"
     CDS             complement(1890..2789)
                     /locus_tag="PKH10_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT27274.1"
                     /translation="MKELVVISGKGGTGKTSIAAALALLAERPVVADCDVDAADLHLL
                     LAPEVKERHEFISGHEAVIRAADCTGCGKCAERCRFDAIEKRVGADGRTDYKVDPVAC
                     EGCGVCVRFCPAHAIDSPDSFCGEWMVSETRIGPMVHARLAAAAENSGKLVSLVRKEA
                     RRVAEEQGRELIIVDGPPGIGCPVIASLTGAHWVLVVTEPTVSGEHDMARVLELAAHF
                     GIPAAVCVNKYDINLDAAVRIESVARDKGAFIVGRIPYDRAVTEAQILGKSVVETGGP
                     AAEAIQRLWGPLKRSLDIMSISA"
     gene            complement(2786..3652)
                     /locus_tag="PKH10_03755"
     CDS             complement(2786..3652)
                     /locus_tag="PKH10_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT27275.1"
                     /translation="MIIAVASGKGGTGKTTVAVNLARSLDRPVHLLDCDVEEPNANLF
                     LHGASERTEEVTLPVPVVDTAKCDGCNECGRFCQYHAIASFGSQALVFPELCHGCGGC
                     MLVCPHGAITETPRRIGVVEMARSGEVTALTGRLDVGVALAPPLIRALTRRIVPETTT
                     ILDAPPGTSCSVVTTVRSADAVVLVTEPTPFGLHDLTLAVDTVRALGVPFGVVVNRVG
                     VGDGRVHAYCAAERIPVLLDISDDRRIAEVYSSGRMMVEALPEYRALFGELWHAVEKL
                     AAKKKGSREAVR"
     gene            complement(3660..4019)
                     /locus_tag="PKH10_03760"
     CDS             complement(3660..4019)
                     /locus_tag="PKH10_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNT27276.1"
                     /translation="MKIACTTTGNDLQAPLDPRFGRAQKLIIFDTDTNAFSVIDNAGG
                     VSAAQGAGIQAAEAVVRAGAVALISGHIGPKAFRALRASNVKMYTTDAPTVAEALARY
                     KAGSLVEAKSADVNGHW"
     gene            complement(4016..4276)
                     /locus_tag="PKH10_03765"
     CDS             complement(4016..4276)
                     /locus_tag="PKH10_03765"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNT27277.1"
                     /translation="MPGRDGTGPMGQGAMTGGGFGPCGSGRGTGRGFFGRGAFCRGGG
                     RGAGMGMSGGGRGFRHWFRATGLTRWMRANMTPQPITPEEKK"
     gene            complement(4539..4733)
                     /locus_tag="PKH10_03770"
     CDS             complement(4539..4733)
                     /locus_tag="PKH10_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27278.1"
                     /translation="MSAVNISSVAGCPVPLGAGDASVPGAGSKMPSSRSSATNSVSSA
                     SGASPRSSRSSVAPAASFSS"
     gene            4756..5724
                     /locus_tag="PKH10_03775"
     CDS             4756..5724
                     /locus_tag="PKH10_03775"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT27279.1"
                     /translation="MPVAATFSVTYRCPLACHHCFFDKKPATAEGELTLPEIERIFDQ
                     LRDAGTLYLTLTGGEPFLRDDLLDIVAAARERRFAVSLLTSGYLCDDRTLDALAALWP
                     ESVQVSLYGPSGATHDDFTKVSGSFDRAIGTLRGLRDRGVAVTAAVSLNTVTAPTVRE
                     LAALMATERIPLSCNLTMLPARSGTTDPTALNLPEEELATLIADLAIPHGDRLAGKLP
                     DDPPCAAARSIVALDPYGTILPCHELDIPAGSLREKPFAELWRTESFENLRRIRFGDL
                     TDCPDCPYRAVCGRCPALALRTGGTITDHAALDCRTARVFKKVFDL"
     gene            complement(5877..6842)
                     /locus_tag="PKH10_03780"
     CDS             complement(5877..6842)
                     /locus_tag="PKH10_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNT27280.1"
                     /translation="MMRFILFPILLIALFGCTKEQPPVVQTESTPAKESVPPVDCRKF
                     MPPWGDEYWEDWGKAHGEQFEKWYMATMPVMKNITVADIEKCAHLDNIFVGFSKLDTL
                     APFAKMPNLRKLDLRFSAGIKDLTPLSGLKDLEELTIWGTGVTDLTPLKDLPKLRRID
                     AKMTQISDLKPVAAMKSLFALDVLMAPVTDIAPLADHPALAEVVLCSTKVPDVAPLYG
                     YAERITYLDLCNTLIPDVTILKKFKNAQKLKLWGLPIKDAKLFSEMKDLRYLDLRNTP
                     VSSLKPLEKLEKIETLIVAETKVSKKEIARFKKLRPAVNVVELVE"
     gene            complement(6859..>7326)
                     /locus_tag="PKH10_03785"
     CDS             complement(6859..>7326)
                     /locus_tag="PKH10_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27281.1"
                     /translation="LGIVALVMGLINIKDYFFFKKGVSLMIPESAKGKLYDRMRRILG
                     SKNTFLAFAGTVALAFFVNLIELGCTIGLPAIYTRVLSIQKIGTAAKYGYMALYNIYY
                     VIPLVLIVALFMFSMQKFRLDERHARVLKLISGILMLVLGLFLVFKPEWLVFL"
BASE COUNT         1440 a         2425 c         2112 g         1349 t
ORIGIN      
        1 gttgaatgtc tggaatatgt aaccgacctc cttcgcgcgc caccggtcgc gatcgcgttc
       61 ggagagtttc gcgagttcga tccctccggc cgttatcgta ccgctgtcgg gcaaaagaag
      121 tcccgatatc agattgaaaa aggttgtctt accggcgccc gacggccccg tgaccgccac
      181 cagcgccccc ggcgcgatgt cggccgagac gccggcgatg acgcgccgcc ggttccccga
      241 cgggtcggtg aacgaccgct caagaccctg caatactatc cgcatgacat tctcctagtg
      301 gccgcaccca ccgccgtgac cgtgaccgcc gcaggtgccg ctcatgagcg agagccgttt
      361 ctgcttgacc agttcgacat tctccttcac cgtcgcagcg ccggtcttgt acacggcgat
      421 acctgccgcg ttgagtttcg aaagcgcccc tgcgccgatg ccgccgagca cgacgatgtc
      481 tatctcgtgg cccgccaacg cctgtaacgg atggcacatg ccgtgatcat gatgctggtc
      541 gctgttctga agcacctcga ccgcgagcgt atcaacatcg acattcacga acagcggcgc
      601 agaaccgaag tggttgtaga cgatgctgtc gagtcctttg ttctcttcta cgggaaagca
      661 tactttcatg accgttcctc ctcggtgata ggtttggtat cgttgcattc gtgttccgtc
      721 ccacagtaac cacagcgctc acggtggccg ccgtgccggt gttcccgcac cccgccgcac
      781 cgcggacagc gacagaaatc gccctcgaca tcgaccgcgc cgccctcgat caccagcgcc
      841 gccccctcga caatggcctg cgccagtttg cggcgcgccg tctcgatgag tcgcgtgaag
      901 gtcggccgcg agatgttcat ccgttcggcc gccgacgcgt gctccagccc ttcgtggtcg
      961 gcgagccgta tcgcctcgaa ctcatcgagc gagagggtca ccttgcgcag gctacggaac
     1021 ggtatcccct gcggcttgaa acggacgaag gcgggcggac gacccacccg gcggcatttt
     1081 ctcgggcgcg gcatcagtga ccgatgacct cccctatcac aacaacggta agcatgccgg
     1141 tcaaaatgag cgggatctcc cagagaaggc tcttcactcc gccggcgacg cgccgccccg
     1201 ggacaagatc ggcgagcgcg atgtagagga aactggccgc cgcgatcgcc aggagatatg
     1261 gaacgaccga cgacacgagc ggaaggagat aataggcggc gagggcgccc ggcagcgaga
     1321 cggcgctcga aagcagattg taggcgaggg cgcgggggcg ggagtagccg ttctcgagga
     1381 ggatggcgaa atcgccgacc tcctgcggca cctcatgggc gatgacggcg accgccgtgg
     1441 tgatgccgac cgggatcgaa gcaaggaagg cggccccgat cgccaccccg tccacgaaat
     1501 tgtggaacgc gtcgcctatc aggatcatcg agccggcgcg ggtatgcacg gggcatggat
     1561 gatcatgctc gtgacagtgg cgccaaagcg tcaccttctc aagcagatag aaccccatga
     1621 tgccgaagag caccgtcggc aggacccgat cactcgggag atgctccatg gcgtgcggga
     1681 tcatcccgaa gaatgcggca ccgagcaggg ccccgatggc gtaaggaatg agcgcacccg
     1741 taaggtttgc aagttggcgc tccttaaaga gtagtacgag ccccgccaca ccgaccgaac
     1801 caagcgagcc cgcaacagtg aacacgatga tgagaaacaa tgtcatgtga agactccact
     1861 actccaaatt caaggtcacg ggggtcatgt tatgcactta tgctcataat gtcaagcgat
     1921 cgcttcaatg gaccccacaa ccgttgtatc gcctccgccg ccggaccgcc cgtctcgacg
     1981 acgcttttac ccagtatctg cgcctcggtg acggcgcgat cgtagggtat ccgccccacg
     2041 atgaacgccc ccttgtcccg cgcaacgctc tctatcctga ccgccgcgtc gagattgata
     2101 tcgtacttgt tgacgcagac cgccgccggt atcccgaaat gagccgcgag ttccagcacc
     2161 cgcgccatgt cgtgttcccc cgatacggtc ggctcggtca ccacgagcac ccaatgcgcg
     2221 ccggtaagcg acgcaatgac cggacacccg atgccgggcg gtccgtcgac gatgatgagt
     2281 tcgcggccct gttcttcggc tacgcgacgc gcctctttcc gcacgagcga caccagtttc
     2341 cccgaattct cagcggcggc ggccagccgc gcatggacca tcgggccgat gcgcgtctcc
     2401 gacaccatcc attcgccgca gaaactatcg ggggaatcga tagcatgcgc cggacagaac
     2461 cggacgcaga caccacaccc ttcgcaggcg accggatcga ccttgtagtc ggtccgaccg
     2521 tcggccccca ctctcttttc tatcgcgtcg aaccggcagc gttcggcgca cttcccgcag
     2581 ccggtgcagt cggcggcgcg gatgaccgcc tcgtgaccgc tgatgaattc atgccgctcc
     2641 ttcacctccg gcgcgaggag cagatggaga tcggccgcgt cgacatcgca gtcggcgacg
     2701 accggccttt cggcaagcag cgccagcgcc gccgcgatgc tcgtcttccc cgtccccccc
     2761 ttgccgctta tcactaccag ttccttcatc gcaccgcctc ccgcgaacct tttttcttcg
     2821 ccgcgagttt ttcgacggcg tgccacagtt cgccgaaaag cgcccggtat tcggggagcg
     2881 cctcgaccat catccggccg ctcgaataga cctcggcgat ccgtcggtca tcggaaatgt
     2941 cgagcaggac cggtatccgc tccgccgcgc agtaggcgtg gacgcgaccg tcgcccaccc
     3001 cgacgcggtt gaccaccacc ccgaacggga caccgagcgc ccgcaccgta tcgacggcga
     3061 gcgtcagatc gtgcaggccg aagggggtcg gctcggtgac gagcaccacc gcgtcggcgc
     3121 ttcggacggt cgtcacgacg gagcacgagg tccccggcgg cgcgtcgagt atcgtcgtcg
     3181 tctccggcac gatgcgccgg gtgagcgccc gaatgagcgg cggcgcgagc gccaccccga
     3241 catcgagccg ccccgtaagg gccgtgacct cgcccgaccg cgccatttca acgacaccga
     3301 tgcgacgcgg cgtttcggtg atggcgccgt gcgggcagac aagcatacaa ccgccgcatc
     3361 cgtggcagag ttcggggaat acgagcgcct gcgacccgaa cgaggcgatc gcgtggtact
     3421 gacagaaccg gccgcactca ttacagccgt cgcatttggc cgtatcgacg accggcaccg
     3481 gaagggtgac ctcctcggtc cgctccgaag caccgtgcag gaacaggttc gcgttcggtt
     3541 cttcgacatc gcagtccaac agatgcaccg gccgatcgag cgaccgcgcc agattcaccg
     3601 cgacggttgt cttcccggtc cctcccttcc ccgaagcgac cgctatgatc atttttttac
     3661 taccagtggc cgttcacatc ggccgacttc gcctcgacaa gcgatcccgc cttgtagcgc
     3721 gccagcgcct cggcgaccgt cggggcgtcg gtggtgtaca tcttgacatt gcttgcccgc
     3781 agggcgcgga acgccttcgg tccgatgtgt cccgatatca gcgcgaccgc gccggcccgg
     3841 accaccgcct cggccgcctg aatgccggcc ccctgcgccg ccgagacgcc gcccgcattg
     3901 tcgatgacgg aaaacgcgtt ggtgtcggtg tcgaaaatga tcagtttctg cgcgcggccg
     3961 aaccgcgggt caagcggcgc ctgcaggtcg ttaccggtcg tcgtacaagc gatcttcatt
     4021 tcttctcctc tggtgtaatg ggttgcgggg tcatattcgc ccgcatccag cgggtcagtc
     4081 cggtcgcacg gaaccagtga cggaacccgc gaccgccgcc gctcatgccc atccccgcac
     4141 cgcgaccgcc gccgcgacag aaagccccgc ggccgaagaa accgcgtccc gtacctctgc
     4201 cgcttccgca cggcccgaag ccgccgcccg tcatcgcacc ctgccccatg gggcccgttc
     4261 catctctacc cggcataaca acacctcctg tgaaagttcg ttggtcacga tttgcatatt
     4321 atgcacctat gttcataatg tcaagccgta tctcatcttt tttcttcgct atttgccgaa
     4381 agaacttaca atacacctgc ttgtttatta ataatctgtg gagaaaaact tctgattttc
     4441 gggcaaatca acagcatcgc atgtcgtccc ggcccgcaca tctcgtatcg cacctacccc
     4501 gaccggacga tactcatcga tcaccgcacc gctacgacct atgaactgaa cgacgcggcg
     4561 ggagcgacgc tcgaccggct cgaacggggc gaagcgccgc tggccgatga aacggagttc
     4621 gtcgccgaac tgcgcgaact cggcatcttc gacccggcgc cgggaacgct tgcgtcacca
     4681 gccccgagcg ggacgggaca accggcgacc gacgagatat tcaccgcact caaacgctac
     4741 ggcgaacagc actgcatccc ggtcgccgcc accttctcgg tcacctaccg ctgtccgctc
     4801 gcctgccacc actgcttttt cgataaaaaa ccggcgaccg ccgagggcga actgacactc
     4861 cccgagatag aacgcatctt tgaccaactg cgtgatgccg ggacgctgta tctcacgctc
     4921 accggcggcg agcccttcct gcgcgacgac ctcctcgaca tcgtcgcggc ggcccgggaa
     4981 cggcgtttcg ccgtatcgct actcacttct ggctatctct gcgacgaccg gacgctcgat
     5041 gcgctcgcgg cgctctggcc cgaaagtgta caggtgtcgc tctacggccc gagcggcgca
     5101 acgcacgacg atttcaccaa ggtctccggc tcgttcgacc gcgccatcgg cactcttcgc
     5161 ggcctgcgcg accgtggcgt cgccgtcacg gcggcggtat cgctcaacac cgtgaccgca
     5221 ccgacagtgc gcgaactggc cgcccttatg gcgaccgaac ggataccgct ctcctgcaac
     5281 ctcacgatgc ttccagcgcg gagcggcacc accgacccga cggcgctcaa cctgcccgaa
     5341 gaagaattgg ccacgctcat cgccgatctc gccatcccgc acggtgaccg tctggccgga
     5401 aaactgcccg acgatcctcc ctgcgccgcc gcgaggtcga tcgtcgcgct ggacccctac
     5461 ggcacgatac tcccctgcca cgaactcgat atccccgccg gttccctgcg cgaaaaaccc
     5521 ttcgccgaac tgtggcgcac cgaatcgttc gagaacctgc ggcgcatccg cttcggcgat
     5581 ctcaccgact gtcccgactg tccctaccgc gccgtgtgcg gtcgctgtcc cgccctcgcc
     5641 ctgcgaacgg gtggcacgat caccgaccac gccgcactcg actgccggac tgcccgagtt
     5701 ttcaaaaaag tgtttgactt ataaaaggat gacgtctata ctgggtccgt tgatgttcat
     5761 ctacgcggag gaacctcatg gctaagaaac cggtgatcgt gaagaaagaa acggtcaaac
     5821 cgaccgcgaa caccaaccag tgccagtact gcaccggcaa gcagatctgc tgaaggtcac
     5881 tcgaccagtt cgaccacatt caccgcagga cgcagtttct tgaagcgcgc gatctctttc
     5941 ttgctcacct tggtctcggc gacgataagc gtttcgattt tttctagttt ttccaatggc
     6001 ttgagtgaag agaccggcgt attgcgcagg tcgagatagc ggagatcttt catctccgag
     6061 aacaacttcg cgtcctttat cggaagcccc cagagtttca gtttctgggc gttcttgaat
     6121 ttcttcagga tcgtgacatc ggggatcaaa gtgttgcaca ggtcgaggta ggttatccgc
     6181 tcggcatagc cgtagagtgg tgcgacatcg ggcactttgg tcgagcagag gaccacctcg
     6241 gcgagcgccg gatgatcggc aagcggcgcg atatcggtga ccggcgccat gaggacatcg
     6301 agcgcaaaaa gacttttcat cgcggcgacc ggcttcaggt cgcttatctg cgtcatcttg
     6361 gcatcgatgc ggcgcagttt cggcaggtct ttcaacggcg tcagatcggt cacgccggta
     6421 ccccagatgg tgagttcttc aagatccttc agccccgaaa gcggcgtcag atcctttatc
     6481 ccggccgaaa aacggagatc gagtttccgg agattgggca tcttggcgaa cggcgcaagg
     6541 gtgtcgagtt tcgagaaacc gacgaagata ttgtcgagat gggcgcactt ctcgatgtcg
     6601 gcgaccgtga tattcttcat caccggcatc gtggccatat accacttctc gaactgctct
     6661 ccatgcgcct tgccccaatc ctcccagtac tcgtcgcccc acggcggcat gaatttcctg
     6721 cagtcgacgg gcggaacaga ttcctttgcg ggagtcgact cggtctgcac aacgggcggc
     6781 tgctctttcg tacaaccgaa caacgcgatc agaaggatcg ggaacaggat aaaacgcatc
     6841 atggtatcct ccttttcttt ataggaacac gagccattcg ggcttgaaga caaggaacag
     6901 gcccagcaca agcataagta tcccgctgat gagtttgagc acccgggcgt ggcgctcatc
     6961 gaggcggaac ttctgcatcg agaacatgaa gagggcgacg ataaggacga gcgggataac
     7021 gtaatagatg ttgtagagcg ccatgtaacc gtatttcgcc gccgtaccga tcttctgtat
     7081 cgacaggacg cgggtatata tcgccggcag accgatggtg cagccgagtt cgatgaggtt
     7141 gacgaaaaag gcgagcgcga cggtcccggc gaaggcaagg aaggtgttct tcgagccgag
     7201 gatacgccgc atccggtcgt agagtttccc tttggccgat tcggggatca taagactgac
     7261 gcctttcttg aagaaaaagt aatccttgat gttgatgagt cccatgacga gggcgacgat
     7321 accgag
//
LOCUS       DAOQUE010000082         7228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_11385_length_7228_cov_4.416004, whole genome shotgun sequence.
ACCESSION   DAOQUE010000082 DAOQUE010000000
VERSION     DAOQUE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11385_length_7228_cov_4.416004"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PKH10_03790"
     CDS             complement(<1..546)
                     /locus_tag="PKH10_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27282.1"
                     /translation="MKQLTATMLAGVFFFSSMTGMGQWSFSSFLHAKSFDLEDEDDLD
                     DESKDEDFDETPEETPKEEKKEESSSSGSGGLDDIDALMSDEPVKKAPAEKSSSEDLD
                     DDDAPADGRSLAFFYFFADSTAEKKAADIVRFVGQYLKERSEYRFFETEARLFNDFSY
                     RKLTDDIPKVEKLLDEGKTLYE"
     gene            717..989
                     /locus_tag="PKH10_03795"
     CDS             717..989
                     /locus_tag="PKH10_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27283.1"
                     /translation="MATDIIRTPEEARRFARVVLSDILAYNGPKIEKGLMEDNLFESL
                     RDELYEGELYYRSRVEKGLCDSTNFFNMAMVDLLIKAKGGLKTPLW"
     gene            983..1927
                     /locus_tag="PKH10_03800"
     CDS             983..1927
                     /locus_tag="PKH10_03800"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNT27284.1"
                     /translation="MVMCDGPISFTLTAGAAERADRLLADLLGVSRTRVQRNIRRQNI
                     LVNGRAVPQNSFARFAARDLIEADIEPLEELTLAPEPVPLDIVFENDLFLIVNKPAGL
                     VVHPAPGHPSGTLMNGVMHHLGAAAGGGVRPGLVHRIDKDTSGLLVVARRPDALEELA
                     ERFAAHDIVRSYTALVRGHPVEPVGTVRTGHARDPHNRLRFAPVEGAERTAVTHYETV
                     AAYPGVSLLRLRLETGRTHQIRMHLKHLNHPILNDDLYGGPCRIGRPAIDALLARHPR
                     QMLHAGELGFTVGGRSWRFEAPLPADMAAVVAALDNGG"
     gene            1945..2805
                     /locus_tag="PKH10_03805"
     CDS             1945..2805
                     /locus_tag="PKH10_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27285.1"
                     /translation="MERKKVLVFETSRTLMRAFELVLRGTVWVIVKATKEEAIFSAIE
                     SSSPDVLLMDRRALSPEMREALHALPIPVIYCAVKRDDDAAEPLFLPRPFSSEELFEA
                     LGTALAWEPALTAEDAPENGVDLSDEFDADEEPVILADPDDPEYVTDMPDEASPVVEA
                     SPVVEASPVVEVPPVVEEPPVVEEPPVVEAQPVVEEPPVPSSPVPSVPVVPSVPDLSL
                     AEKVEERVLAEVLPADIDIPEGSPMPDVIRAIIRRELREVMKEYFWEEAPQLMRQVVE
                     EEIKKVAAKQ"
     gene            2802..3248
                     /gene="ruvX"
                     /locus_tag="PKH10_03810"
     CDS             2802..3248
                     /gene="ruvX"
                     /locus_tag="PKH10_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNT27286.1"
                     /translation="MTAGRIVSLDIGVKMIGVAVADPLGIIATPLGAVRRGQAIADDI
                     AALKDILKSYEIARFVVGWPLKSDGTESENLPVVKGFEKKLKAAFPGVPVEHENELLS
                     TRVARERISHSPKGRTDKKSGRLDAAAAAVILESYLARHAVSAPGA"
     gene            complement(3297..6095)
                     /locus_tag="PKH10_03815"
     CDS             complement(3297..6095)
                     /locus_tag="PKH10_03815"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT27287.1"
                     /translation="MRSIIFVVSFFLLFLTSCDGGGGNNTNDNAVTGDTDTTTDGDAV
                     TGDELIADDDSLTGDDPATDSDAPQFTIAGFVQKGPFVQGSEITIQELDASLVPIGTS
                     YTTDTIDDFGSFGIARQMASNFIEVTASGFYFNEVVGSLSAAGIQFRVVADIGEAKPV
                     NINILTTIERERIKELIGKGDSFEEARAKAEQEILAIFNITGFETANFQEMDISKEGD
                     SNAILLAISAVLQQGNSEAQLSELISKITQDIKTDGTLDNETQRAKIVEGGIALDLAA
                     VRTNIEDRYTDLGLTDLTIPPFEDFVDSDGDGLINRFDFTAVFAPVTNADLAAVHTSN
                     AITVVLPPAIAEATATVDAGTLVVNGTESGATATVTDGDTIAIRLTAPTTHGETATAN
                     LTVSYDPYEVTGAFAVTAKSDKYFALEFTAVSNAELLQTYTSNEQTITLPATIADAAA
                     TLDNGVILVNGVAKGASATVVNGDTVAVRLNAPTTHGATATANLTVSYGSDEVSGAYA
                     ITARTDKYFTLSFTAKSDADLAQNYTSNEQTVTLPATIADAAATLDHGAIVVNGVAKG
                     ASATVVNGDKVAVRLSTVAYDTAVIGTLTINYLGNTVSGAFSVTAPRMLCGTETCADR
                     LIGLVWTKDEVNAGDGWGDARDSCLKLELGGKSDWRLPKVWEFRTIIRGCPADAETGG
                     SCGVIESCLTGNCMSIGSCSCTGGNTVFNEPSYIWAMNQPMQGGFWTGSVVTDDTAKV
                     WAALPANGPAIGMAGKTNPYTHARCVRSDTDVDEEYSDIYTDPVTGLQWTREAAYITG
                     PTTVTHCADLTLEGKDDWRLPTISELRTLVRDCPNLVTDGACGVTDSCLSGMTCLDIP
                     SCYSCGEGSAGTGPDDGYYWEPGVWIYQNDPKNIYLSSSTDPDNGNPWTLDFYNGSIR
                     INPGPATTGDFMIRCVRN"
     gene            complement(6245..7186)
                     /locus_tag="PKH10_03820"
     CDS             complement(6245..7186)
                     /locus_tag="PKH10_03820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT27288.1"
                     /translation="MTHERRPGESTGVVRATVPSPTPEDPALYRALSRWSRTIPENTV
                     AAISFLLLCAIAVIDLLTGYEVAFSLFYVAPVAITAWRSSIRTGLLLATLSAAVWAMA
                     DHWAGHEYSHWLVLVWNSAVRLSFFAIIALLIAKLHGNLILLDRLARTDPLTGALNGR
                     AFLELAGREIEQARRYGGTFAILFIDLDNFKAVNDRCGHAGGDALLCAIVDALRGTLR
                     ATDLVGRVGGDEFAVWLSHSSGDEARATALRLQRAVADAVAHSDCPTTLSIGAVSVPE
                     VGHDLTLDGLIKKADVLMYRVKNGSKNGLLLESWRPD"
BASE COUNT         1289 a         2194 c         2391 g         1354 t
ORIGIN      
        1 ctcgtagagc gtcttgcctt cgtcgagcag tttctcgacc ttggggatat cgtcggtcag
       61 tttgcggtac gagaagtcgt tgaacagccg cgcctcggtc tcgaagaagc ggtattccga
      121 ccgttccttg agatactggc cgacgaaacg gacgatgtcg gcggcctttt tctcggcggt
      181 gctgtcggcg aagaaataga agaaggcgag cgagcgaccg tctgcggggg catcgtcgtc
      241 gtcgaggtcc tccgaggaac tcttctccgc aggcgccttt ttcaccggct cgtcgctcat
      301 cagcgcgtct atatcatcaa gaccgccgga accggaagag gaactctcct ctttcttttc
      361 ttctttcggc gtctcttccg gggtctcatc gaaatcctca tccttgctct catcgtcgag
      421 atcgtcctcg tcctcgaggt cgaaactctt cgcgtgcaaa aaagaggaga acgaccactg
      481 acccataccg gtcatcgagg agaagaagaa cactcccgcg agcatggtcg ccgtcaactg
      541 tttcatcgtg ggtcctcccg gcgcagggcc gatcgttcaa atagttgccg gttgtgtaac
      601 aaaaaaaaga gggcgtgtca acattttata gccccggatc tttttgcaaa tatccggata
      661 atgggtataa tcggcccgtt cagcggtcat gaacggacgg cacaggaggc atggcgatgg
      721 cgactgacat catccggacg cccgaggagg cgagacggtt cgcgcgggtg gtcctctcgg
      781 acatactcgc ctacaacggt ccgaagatag agaaggggct catggaggac aacctgttcg
      841 aatcgcttcg cgacgaactc tacgaagggg aactctacta ccgttcgcgc gtcgagaagg
      901 ggctgtgcga ttcgaccaac ttcttcaaca tggcgatggt cgatctgctg ataaaggcaa
      961 aaggagggct caagacgccg ctatggtgat gtgcgacggg cccatatcgt tcacgctcac
     1021 cgcgggcgcc gcggaacggg ccgaccgcct gctcgccgac ctgctcggcg tgtcgcggac
     1081 ccgcgtccag cgcaacatcc ggcggcagaa catccttgtg aacggccgcg ccgtaccgca
     1141 gaattccttc gcgcgcttcg cggcgcgcga cctcatcgag gccgacatcg agccgctcga
     1201 ggagttgacg ctcgcgcccg aaccggtccc gctcgatatc gtgttcgaga acgatctgtt
     1261 cctcatcgtc aacaagccgg cggggctggt ggtccacccg gcgccgggcc acccgtcggg
     1321 gacgctcatg aacggcgtca tgcaccatct cggggccgcg gccggcggcg gggtccgacc
     1381 ggggctcgtc caccgcatcg acaaggacac ctcgggcctg ctcgtcgtcg cgcgccgccc
     1441 cgacgcgctc gaggaactcg ccgagcggtt cgccgcccac gacatcgtcc gttcctacac
     1501 ggcgctcgtc cgcggccatc cggtcgaacc ggtcgggacg gtgcggaccg gccacgcccg
     1561 cgacccgcac aaccggctcc ggttcgcgcc ggtcgagggg gcggaacgga cggcggtcac
     1621 ccactatgag acggtcgccg cctatcccgg cgtgtcgctg ctcagactgc gcctcgaaac
     1681 ggggcggacc caccagatac ggatgcacct gaaacacctc aaccacccga tactgaacga
     1741 cgacctgtac ggcggtccct gccggatcgg tcgcccggcg atagacgctc tgctcgcgcg
     1801 ccatccgcgc cagatgctgc acgccgggga actcggcttc acggtcggcg ggcggtcgtg
     1861 gcgcttcgag gcgccgctcc cggccgacat ggcggcggtc gtcgcggcgc tcgacaacgg
     1921 cggataaccg gaaggagata gcgtatggaa cggaaaaaag tgctcgtgtt cgagacctcg
     1981 cggacgctga tgcgcgcctt tgaactggtg ctccgcggca ccgtgtgggt catcgtcaag
     2041 gcgaccaagg aggaagcgat attctcggcg atcgagtcat cgtcgcccga tgtcctgctg
     2101 atggaccggc gggcgctctc ccccgagatg cgcgaggcgc ttcacgcgct tccgataccg
     2161 gtcatatact gcgcggtgaa acgcgacgat gatgcggccg agccgctgtt cctgccgcgt
     2221 cccttctcga gcgaagaact gttcgaggcg ctcgggacgg cgctcgcgtg ggaaccggcg
     2281 ctgaccgcgg aggatgcgcc ggagaacggc gtcgacctgt cggacgaatt cgatgccgac
     2341 gaggagccgg tgatactcgc cgaccccgac gatccggagt atgtcaccga catgcccgac
     2401 gaagcgtcgc cggtcgtcga ggcgtcgccg gtcgtcgaag cgtcgccggt cgttgaggtg
     2461 ccgccggtcg tcgaagagcc gccggtcgtc gaagagccgc cggtcgtcga agcgcagccg
     2521 gtcgtcgaag agccgccggt cccctcttct cctgtccctt ctgtccctgt tgtcccttcc
     2581 gtccctgatc tttcccttgc cgaaaaggtc gaagagcgcg tcctcgccga ggtgctcccc
     2641 gccgacatcg acatccccga gggctcgccg atgcccgatg tgatccgcgc catcatccgc
     2701 cgcgaactgc gcgaggtgat gaaggagtat ttctgggaag aggcgccgca actcatgcgg
     2761 caggtggtgg aagaggagat caaaaaggtc gcggccaagc aatgaccgct ggtcgtatcg
     2821 tttcgctcga catcggtgtc aagatgatag gggtggcggt cgccgatccg ctcggtatca
     2881 tcgcgacgcc gctcggcgcg gtgaggcgcg gtcaggcgat agccgacgat atcgcggcgt
     2941 tgaaagatat cctgaaaagt tatgagatag cgcggttcgt cgtcggctgg ccgctcaagt
     3001 ccgacgggac cgaaagcgag aacctgccgg tcgtgaaggg gtttgagaaa aaactgaagg
     3061 cggcgttccc cggcgtcccg gtggaacacg agaacgaact gctctcgacc cgcgtcgccc
     3121 gcgaacggat atcacactca ccgaaaggga ggaccgataa aaagagcggt cgtctcgacg
     3181 cggcggccgc cgcggtcata ctggaaagtt atctggcacg ccacgcagtg agtgcgcccg
     3241 gggcgtagaa cgccgtaacg gcgcatccat aatcgatttt cgagataagg gaaagatcag
     3301 ttgcggacgc agcggatcat gaagtcgccg gtggtggcgg ggccgggatt gatgcggatg
     3361 gatccattgt agaaatcgag tgtccatgga ttgccgttgt cgggatcggt ggacgaggag
     3421 agatagatgt ttttcggatc gttctgatag atccataccc ccggttccca gtaatagccg
     3481 tcatcgggtc ccgtcccggc cgatccttcg ccgcaagagt agcaggaagg gatatcgaga
     3541 caggtcatgc cgctgaggca ggaatcggtc acgccgcagg cgccgtcggt cacgaggttg
     3601 ggacagtcgc gcacgagggt ccgcagttcc gatatcgtcg ggaggcgcca gtcatctttc
     3661 ccttcgagcg tcaggtcggc gcagtgggtc accgtcgtgg gccccgtgat gtatgccgct
     3721 tcccgcgtcc actggaggcc ggtgaccggg tcggtgtaga tgtccgaata ttcttcgtcc
     3781 acatcggtgt cgctccggac acagcgggcg tgggtatagg ggttggtctt gcccgccatc
     3841 cctatcgcgg ggccgttggc cggaagcgcc gcccatactt tggcggtgtc gtcggtcacg
     3901 accgaaccgg tccagaaacc gccctgcatc ggctgattca tcgcccagat gtaggaaggt
     3961 tcgttgaaga cggtgttgcc ccccgtgcag gagcaggagc ctatgctcat gcagttcccg
     4021 gtcagacaac tttcgatcac gccgcaggac ccgcccgttt cggcgtcggc gggacagccg
     4081 cggatgatgg tgcggaactc ccacactttc gggagccgcc agtcgctctt gccgcccagt
     4141 tcgagtttca ggcaggaatc gcgcgcgtca ccccagccgt cgccggcatt gacctcgtcc
     4201 ttcgtccaga ccagcccgat cagacgatcc gcgcaggttt ccgtgccgca gagcatccgc
     4261 ggggccgtca ccgagaacgc gccgctgacc gtgttgccga gatagtttat cgtgagcgtg
     4321 ccgatgaccg ccgtgtcata ggcgaccgtc gagagccgca ccgccacctt gtcgccgttc
     4381 acaacggtcg ccgatgcgcc cttggcgacg ccgttgacga cgatcgcgcc gtggtcgagc
     4441 gtcgcggcgg catccgctat cgtggcggga agcgtgaccg tctgttcgtt ggaggtgtag
     4501 ttctgagcga gatcggcgtc gctcttcgcg gtgaaggaga gcgtgaagta tttgtcggtc
     4561 cgcgccgtta tcgcgtaggc gccgctgacc tcgtcggagc cgtaggagac ggtcaggttc
     4621 gccgtcgccg tcgcgccgtg ggtcgtcggc gcgttcagcc gcaccgccac cgtatcgccg
     4681 ttcacaacgg tcgccgatgc gcccttggcg acgccgttga cgaggatcac gccgttgtcg
     4741 agcgtcgcgg cggcatccgc tatcgtggcg ggaagcgtta tcgtctgctc gttggaggtg
     4801 taggtctgca ggagttcggc gttgcttacc gcggtgaatt cgagcgcgaa gtatttgtcg
     4861 ctcttcgccg ttaccgcgaa ggcgcccgtc acctcgtagg ggtcgtagga tacggtcagg
     4921 ttagccgtcg ccgtctcgcc gtgggtcgtc ggcgcggtga gccgtatcgc gatggtgtcg
     4981 ccgtccgtca ccgtggcggt cgcgccgctt tcagtaccgt tcacgacgag cgttccggcg
     5041 tccaccgtcg cggtcgcctc ggcgatggcg gggggaagca ccaccgtgat cgcgttggag
     5101 gtgtggaccg cggcgaggtc ggcgttggtg accggcgcga agaccgcggt gaagtcgaac
     5161 cggttgatga gtccgtcgcc gtcgctgtcg acgaaatcct cgaagggcgg tatcgtgagg
     5221 tcggtgagtc ccagatcggt atagcgatcc tcgatgttgg tccggaccgc cgcaagatcg
     5281 agcgcgatgc ccccctcgac tatcttggcc cgctgggtct cgttgtcgag cgtgccgtcc
     5341 gttttgatat cctgcgttat cttcgagatg agttcggaca gttgcgcctc gctgttcccc
     5401 tgctggagca ccgccgagat ggcgagcagt atcgcgttgc tgtcgccctc cttgctgatg
     5461 tccatctcct gaaagttcgc cgtctcgaag ccggtgatgt tgaagatggc gagtatctcc
     5521 tgctccgcct tggcgcgcgc ctcttcgaac gagtcgccct tgccgatgag ttccttgatc
     5581 cgttcccgct cgatggtggt gaggatgttg atgttgaccg gctttgcctc gccgatatcg
     5641 gcgacgacgc ggaactgtat cccggcggcc gagagggagc cgaccacctc gttaaagtag
     5701 aagccggagg cggtgacctc gatgaagttc gacgccatct gccgcgcgat cccgaacgaa
     5761 ccgaagtcgt cgatggtgtc ggtagtgtag gaggtgccga tcgggacgag cgacgcgtcg
     5821 agttcctgga tcgttatctc ggatccctgc acgaatggcc ccttctggac gaacccggcg
     5881 atggtgaact gcggggcgtc gctgtcggtc gccggatcgt cgccggtcag cgagtcgtcg
     5941 tcggcgatga gttcgtctcc cgtgacggca tcgccgtcgg tcgtcgtgtc ggtgtcgccg
     6001 gtaacggcgt tgtcgttcgt attgttaccg ccgccgccgt cgcaggaggt caggaacaac
     6061 aaaaagaaag aaacaacgaa aatgatcgat cgcatgggaa cctccatcgt gaaattcaga
     6121 tgcactaacg cgttgcgtcg agaaactatg cccgatggga ggggggtgcg tcaagaaaaa
     6181 aagaaaacat taagcggata tgggcggccg atttctttcg cgatcctgaa gatgcgagga
     6241 aagatcaatc gggtcgccac gattccagca gcagcccgtt cttggaaccg ttcttgacgc
     6301 gatacatgag cacatctgcc tttttgatca ggccatcaag cgtgagatcg tgtccgacct
     6361 cggggacact caccgcgccg atgctcaagg tggtcggaca gtcgctgtga gctaccgcgt
     6421 cggccacggc gcgttgcagg cgcagtgccg tcgcgcgcgc ctcgtcgcct gaactgtgcg
     6481 aaagccagac ggcgaactcg tcgccgccga cacggccgac aaggtcggtg gcgcggagcg
     6541 tgccgcgcag cgcgtcgacg atcgcacaga gcagcgcatc gcctccggca tggccacaac
     6601 ggtcgttgac cgccttgaag ttgtcgagat cgatgaacag gatcgcgaag gttccgccgt
     6661 aacggcgcgc ctgttcgatc tcgcgtcccg ctagttcaag aaaagcgcgg ccgttgagcg
     6721 cgccggtgag cggatcggtg cgtgcgagcc gatcgagcag gatcagattc ccgtgcagtt
     6781 tggctatgag cagggcgatg atggcgaaga aggagaggcg cacggcgctg ttccagacaa
     6841 gcaccaacca gtgggaatac tcgtggcccg cccagtggtc ggccatggcc cagaccgccg
     6901 cgctgagcgt tgcgagcagg agcccggtcc ggatcgagga gcgccatgcg gtgatggcga
     6961 ccggcgcgac atagaacagc gagaaggcga cctcgtagcc ggtgagcagg tcgatgacgg
     7021 cgatcgcgca gagtagcagg aaagagatcg cggcaacggt attctcgggt atggtgcgcg
     7081 accagcggga gagagcccgg tacagggccg ggtcttccgg tgtcggtgaa gggacggtcg
     7141 cgcgcactac tccggtcgac tctccgggtc gtcgttcgtg ggtcataggt ggtcccccgt
     7201 gcggaaatca ggggaccact atacctct
//
LOCUS       DAOQUE010000083         7208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_11425_length_7208_cov_3.841605, whole genome shotgun sequence.
ACCESSION   DAOQUE010000083 DAOQUE010000000
VERSION     DAOQUE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11425_length_7208_cov_3.841605"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="PKH10_03825"
     CDS             <1..1575
                     /locus_tag="PKH10_03825"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT27289.1"
                     /translation="TGADLAFFNTVPDLDGPIRHYLLVSSVRQGEDEWVLLPSLALAA
                     TQLYFDKVAKITYSTTGIEEIRVGELVIPTSYEGLSLINYYAKSDRFKLFSIADMAAA
                     DVKDKVVFVANTALGTWDQRVTPLEEFIPGIYVHAAMMNNILEGRFITAPFWSYLVSF
                     LFVLIPLFFIRFLYHRVPLWQGIAGTAIYSVLFFLFGYFSHKGGVVIYMATPLLFLWF
                     LMLLVYIGKYFFVDKQASFMRNAFAKYLDPKLLSQLTGDPDQIQLYGRKKEIVALFSD
                     IVGFTTLSESMPPEDLIKAINRLFTPATAAVLEHSGFLDKYMGDAMMALYGAPIEIAN
                     KEKEACRSAVKIMERMDIVRAEFKAEGKPDVRMGVGVNAGDAIVGNMGSEERFNYSAI
                     GDAVNTASRMEGLTRPYGVRIIASEKIFERAQDEFLFRHLDTVQVKGKKLGVRIFELM
                     GRLSDAGIEEKRKLKEQYEAALGPFIAGDMAKCLAACEKSTFPDDKAFAFLKDRAQEM
                     KESGEVYNGIWEFKSK"
     gene            complement(1576..1989)
                     /gene="arfB"
                     /locus_tag="PKH10_03830"
     CDS             complement(1576..1989)
                     /gene="arfB"
                     /locus_tag="PKH10_03830"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015150635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNT27290.1"
                     /translation="MLRITATVAIPLSEIEFTAIRAEGPGGQHVNRTSSAVQIRFDIE
                     ASSLPDEMKRRLRARRDHRITAAGVIIIKVQDERSLTRNKETALERLADLIRRAIAVP
                     KKRKPTKPTKSSVEKRIAGKKKRGETKRLRRLFAD"
     gene            complement(2184..2966)
                     /locus_tag="PKH10_03835"
     CDS             complement(2184..2966)
                     /locus_tag="PKH10_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27291.1"
                     /translation="MSEKTRNTFPGNIHRPTNIGKEIPSCRTIRRYMDLWKFKSIINK
                     KSLYFRRADKLEDEFEGSSGEWYARVVEEIRKSKSISIEIPAIITDVRKYLRASTYVN
                     CWRIEEVEDALMWRGYTKSNCAVAIETNVALLECSFPYNCATTSDIARGVVDYIDYEK
                     DPVDISDIRQPFVKKRLEYKSENELRLIYFKKKVFPVLKQKYSGVDGRWLPIDFKTLI
                     RKVIIKPQAPQCCEDAVRDILSNNGLDENSVTRSALEKKPVF"
     gene            complement(3102..6569)
                     /locus_tag="PKH10_03840"
     CDS             complement(3102..6569)
                     /locus_tag="PKH10_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27292.1"
                     /translation="MRIAFFFFCALFVLMFAACDDNKPNGTTDNDTTTPAVGSEGGPC
                     YPNGTCNDGLQCLSDICVEMPDALNDETTADEDEIATEDAPDKTESVIPDDDQQLIED
                     DILPDSDTYVAKCGNDNIDDGETCELGDTIDCALVPDKNFASGTTASCNNSCTGWLDI
                     VCTTCGNGAKDDGELCEKGTTLDCASIPGKSYETGTLATCNASCTGWNDKDVCTVETP
                     ITCNNGTFDDGSELCEAGFETSCADIPGMNYADSGNAVCNDTCTGWNDEVACTTCGNG
                     VKDGGETCEKGTIIDCGSIAGKNYETGTDATCNDTCTGWNDAAVCTLKPIETCDNGTY
                     DPGLEVCEKGLTTPCEEIPSANYAAVGTATCNDTCTGWNDATACTTCGNGAKDASEVC
                     DGSATLSCATVFGNYTGDTACLDDCTGYDITDCTAATRTYTCTDKPATGTAWNTVSSY
                     TQTWSGSAWLPVDDPTTEYNATASTTSCRYKCATNYTWNGTACAANTRTYTCSVKPAS
                     NTAWNTVSSYTQTWNGSEWMPVDDPLTEYNETGSATSCQYKCATNYTWNGSSCVAGTK
                     TYTCNAKPATGTVWNTVSSYIQTWTGTTWNPADDAVTDYNVTGSATSCRYKCDINYTW
                     SGSVCVANTRPYNCSVKPSIGTIWNTVSSYTQTWNGSEWMPVDDSTTEYNETGSATSC
                     QFVCATNYNWTGTECEAATKNFSCNAIPTANGVYNTAPSYSQTWSGTAWEPADDAVTE
                     YNATPSTTSCRFVCDAGYVWNSVTQTCPVADICSNGVVETGEECDTYDTTCNDISTAF
                     NYGVAPCSELCDVLDTNLCMYKSSFAGLISSIDVDNNENVYIVKNLYPSQGIFLYKYN
                     NAGSLVWNKSLQSVDEGGLYSPSYGAVDSQNKIYATSMIKAGTDQYTGVFLIKYNEDG
                     SEAWHTMTGTGDSLDEEVSAITIDASDNIYVAGKYSSQSKIFVYMFNSSGTVLRKVYL
                     DNLANHYADVAVDSAGLIYALGYNEQTCRMAIFDSNASPKCSYTWSTTTYSYQAITVD
                     SNGNPYVAGVHELAVGREAVLTKMTAACEISSHIGWDSGRYLNSFSVAFDVTADSDNN
                     FYMGGYTVVEGGNKDYFVAKWDVSGAMLWQYVDGEPAQEKLTTMALDNNKHLYIAGEE
                     IPYTKINNN"
     gene            6839..>7208
                     /locus_tag="PKH10_03845"
     CDS             6839..>7208
                     /locus_tag="PKH10_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNT27293.1"
                     /translation="MSGHGEGGPVKGLPENAYRELKAGEVYQPVMPAAENVQEVTRWS
                     LFMGLLMAAVFSAAAAYSGLKIGQVFEAAIPISIIAVGASAALRKKNVLGQNVIIQSI
                     GAASGVIVAGAVFTIPGIYIL"
BASE COUNT         1554 a         1979 c         1845 g         1830 t
ORIGIN      
        1 accggcgccg accttgcctt cttcaatacc gttcccgatc tcgacggtcc gatccgccac
       61 tacctcctcg tgagttcggt ccggcagggg gaggatgaat gggtgctcct gccatcgctc
      121 gcgctcgccg cgacacaact ctacttcgac aaagtggcga agatcaccta ctcgaccacc
      181 ggcatcgagg agatacgggt cggcgaactc gtcataccga ccagttacga ggggctttca
      241 ctcatcaact actatgcgaa gagcgaccgg ttcaagctgt tctcgatcgc cgacatggcg
      301 gcggccgatg tgaaggacaa ggtggtcttc gtagccaaca cggcgctcgg gacctgggac
      361 cagcgcgtaa cgccgctcga ggaattcatc cccggcatct atgtccacgc cgcgatgatg
      421 aacaacatcc ttgaagggcg cttcatcacc gccccgttct ggtcctacct cgtctcgttc
      481 ctcttcgtcc tcatcccgct cttcttcatc cggttcctct atcaccgtgt cccgctctgg
      541 cagggtatcg ccggtaccgc catctattcg gtactctttt tcctgttcgg ctatttctcg
      601 cataagggag gtgtcgtcat ctacatggcg accccgcttc tgttcctgtg gttcctgatg
      661 cttcttgtct acatcggcaa gtatttcttc gtcgacaaac aggcctcgtt catgcgcaac
      721 gcctttgcaa agtatctcga cccgaaactg ctctcgcaac tgaccggcga ccccgatcag
      781 atacaactct acggccgcaa gaaggagatc gtcgccctct tttccgacat cgtcggcttc
      841 acgaccctct ccgagtcgat gccgcccgag gacctcatca aggcgatcaa ccgcctcttc
      901 accccggcga ccgccgcggt gctcgaacac agcggtttcc tcgacaagta catgggcgac
      961 gcgatgatgg cgctttacgg cgcacccatc gagatcgcca acaaggaaaa ggaggcgtgc
     1021 cgttcggcgg tcaagatcat ggagcggatg gacatcgtcc gcgccgagtt caaggccgag
     1081 gggaaacccg atgtacggat gggggtcggc gtcaacgccg gcgacgccat cgtcggcaac
     1141 atgggctccg aggagcggtt caattactcc gccatcggcg atgcggtcaa cacggcgtca
     1201 cgcatggaag ggctgacccg tccctacggc gtccgcatca tcgcatcgga gaagatattc
     1261 gagcgggcgc aggacgaatt cctgttccgc catctcgaca ccgtgcaggt gaaggggaag
     1321 aaactgggcg tccgcatctt tgaactgatg gggcggcttt ccgacgcggg gatagaggag
     1381 aagcggaaac tcaaggagca gtacgaggcg gcgctcggcc cattcatcgc gggcgacatg
     1441 gccaaatgtc ttgccgcgtg cgagaagagc accttccccg acgacaaggc gttcgcgttc
     1501 ctcaaagatc gcgcccaaga gatgaaagag tcgggtgaag tctacaacgg catctgggaa
     1561 ttcaagtcga agtgatcaat cggcgaagag gcggcgcagc cgcttggtct cgccccgttt
     1621 cttctttccg gcgatcctct tttccaccga acttttcgtg ggcttggtcg gtttccgctt
     1681 cttggggacc gcgatagcgc gccggatgag atcggccagc cgttccagcg ccgtctcctt
     1741 gttgcgggtg agcgaccgct catcctgcac tttgatgatg atgacgccgg ccgccgtgat
     1801 gcggtggtcg cgccgggcgc gcagtcgccg cttcatctcg tccgggagcg acgatgcctc
     1861 gatatcgaac cgtatctgca ctgcgctcga tgtccggttc acatgctgac cgccggggcc
     1921 ttcggcgcgg atcgccgtga actcgatctc ggagagcggt atcgcgaccg tcgcggttat
     1981 gcgcagcatg ggacctccaa caaacaaggg acgatggtag cgcaacacgg aaggaaggtc
     2041 aaactaatat gtcagagaga taaaagagaa gaaaatcttg aatatagggg gtaaccttcg
     2101 cgcccggaga aaagagagga gatgaatcaa atgaaaaact agcccgtaat aaaaacaacc
     2161 aaaacaagag cacccaacca aatctaaaat acaggttttt tttctaatgc agatcttgtc
     2221 actgaatttt catcaagccc gttgtttgac aaaatatctc tgaccgcatc ttcgcaacac
     2281 tggggtgcct gtggtttaat gatcactttc cgaataagag ttttaaagtc aatagggagc
     2341 catcgtccat caacaccgga atacttttgt tttaagacgg ggaagacttt ctttttgaaa
     2401 tatataagtc ttaactcgtt ttccgattta tattccaacc tttttttgac aaatggttgc
     2461 cttatgtcag agatgtcaac ggggtctttt tcgtaatcaa tatagtcaac cacacctcgc
     2521 gctatatcgc tagtggtcgc acaattataa gggaatgagc attccaacag cgcgacatta
     2581 gtttcaatgg caacggcgca atttgatttt gtatacccac gccacattag agcatcttcc
     2641 acctcttcta tgcgccagca atttacatag gtagacgctc taagatattt tctaacatcc
     2701 gttattatgg cgggtatttc tatagagatg cttttgcttt ttcttatttc ttctacaact
     2761 ctcgcgtacc attctccgct tgatccttca aactcatcct cgagtttatc tgctcgacga
     2821 aagtacaggc tttttttgtt tattattgac ttgaatttcc acaaatccat atatcttctg
     2881 atcgttctac aagacggtat ttctttccct atattggtcg ggcggtgtat gtttccggga
     2941 aaagtgttcc gagttttctc tgacataata cctgcctctt ttgttttttc atccgattaa
     3001 aagtgcctat tgcgggaggt tctagagcaa attgatcggt cattccggaa caatcgactg
     3061 ttgccatgac cgtttttcaa tcattatcat ctcttccctt gtcagttatt gtttatcttc
     3121 gtgtatggta tctcttctcc tgcgatatat agatgcttgt tattgtctag cgccattgtg
     3181 gtcagttttt cctgcgctgg ctcgccatcg acatattgcc ataacatagc gccgctgaca
     3241 tcccacttgg ctacaaaata gtctttgttc ccgccctcga caacagtata tccccccata
     3301 taaaaattgt tgtcagagtc tgctgttaca tcaaaagcaa cgctaaacga attgagatat
     3361 ctgccactgt cccatccaat atgacttgat atttcacaag ctgctgtcat ttttgtaagc
     3421 acggcttctc tgcctactgc caattcatga accccggcga cataaggatt gccattgcta
     3481 tcgactgtta ttgcttgata gctataagta gttgtgctcc aagtgtagct gcatttaggg
     3541 gaagcattgg aatcaaaaat agccattctg catgtttgct cattgtagcc cagagcgtaa
     3601 attaatcccg cactgtcaac cgcaacatcg gcataatgat tggccagatt gtccaaatat
     3661 accttgcgca gtaccgtgcc cgaagaattg aacatgtata cgaagatctt gctttgtgat
     3721 gagtatttgc cagctacata tatgttgtct gaagcatcta tcgttatggc tgacacctct
     3781 tcgtctaaag aatcgcccgt ccctgtcatg gtgtgccacg cttctgaacc atcttcatta
     3841 tatttgatca agaaaactcc cgtgtattga tcggtaccgg ctttaatcat gcttgtagca
     3901 taaattttat tttggctatc tacggcacca taacttggag aatacaaccc tccctcatca
     3961 acgctttgca aagatttgtt ccaaacaaga gagccggcgt tattatactt atatagaaag
     4021 atgccctggc tgggataaag gtttttcaca atatagacat tttcgttgtt atccacgtct
     4081 atagaagata tcagaccagc aaaggagctt ttatacatac aaagatttgt gtccaaaaca
     4141 tcgcaaagtt cgctgcaagg agcaacgcca tagttgaatg ccgtgcttat atcgttgcaa
     4201 gtcgtgtcgt aggtatcgca ttcttcacct gtctcgacaa cgccgttgct acatatatcc
     4261 gcgaccggac atgtttgcgt cacgctgttc cagacatagc ccgcgtcgca gacgaaccgg
     4321 catgaagtcg tgctcggcgt tgcgttgtat tccgtcaccg catcatctgc cggttcccac
     4381 gccgtgccgc tccatgtctg cgaataagac ggtgccgtgt tgtagacgcc gttcgccgtc
     4441 ggtatcgcat tgcaactgaa attctttgtc gccgcctcgc actccgttcc tgtccagttg
     4501 tagttcgtcg cgcagacaaa ttggcaactg gtcgcgctac ccgtctcatt gtattcggtc
     4561 gtcgaatcat caacgggcat ccattcggag ccattccagg tttgcgtgta actcgacacc
     4621 gtgttccaaa ttgtaccgat agatggtttt accgaacagt tatacggccg cgtgttcgcc
     4681 acacagacag aaccactcca agtgtagttg atatcgcatt tatagcggca ggaagtggcg
     4741 ctgccagtaa cattataatc ggtcaccgcg tcatcggcgg ggttccaagt cgtgccggtc
     4801 catgtctgta tataactcga caccgtgttc caaacggttc cggtcgccgg tttcgcgttg
     4861 caggtgtagg tcttggttcc ggcaacacag gatgaaccgt tccaagtgta attcgtggcg
     4921 cacttgtatt ggcaactggt tgcactgccc gtctcgttgt attcagtcaa cggatcgtca
     4981 acgggcatcc actcggagcc attccatgtt tgcgtgtaac tcgacaccgt gttccatgca
     5041 gtgttgctgg ctggcttcac cgaacaggta taggtgcgag tgttcgcagc gcacgcggtg
     5101 ccgttccatg tgtagttcgt ggcgcatttg tagcgacagg atgtcgtact tgcggtcgcg
     5161 ttatattcgg tcgtcggatc gtcgaccggc agccatgccg aaccgctcca tgtctgcgtg
     5221 taactcgaca cggtattcca cgccgtgccg gtagcgggct tgtcggtgca agtgtaggtc
     5281 cgtgtggcgg cggtacagtc cgtaatgtcg tacccggtac agtcatcgag gcaagccgtg
     5341 tcgccggtgt agttaccaaa gacggtggcg caagacaggg tcgcggaacc atcgcaaact
     5401 tcgctggcat ccttggcgcc attgccgcaa gtggtgcaag ccgtcgcgtc attccagccg
     5461 gtgcaggtat cgttgcaggt cgccgttcca acggcggcat agttcgcgga cggaatctct
     5521 tcgcagggtg tggtcagtcc tttctcacag acctccaacc ccggatcata ggtgccgtta
     5581 tcgcaggtct ctatcggctt gagagtacag accgctgcat cgttccaacc ggtgcaggtg
     5641 tcgttgcagg tggcatctgt gcctgtttcg tagttcttcc ccgcgatgct cccgcagtct
     5701 atgatcgttc ccttctcgca ggtttcgccg ccgtccttga cgccgttgcc gcaggtcgtg
     5761 caagcgacct catcgttcca gccggtgcaa gtgtcattgc agacggcgtt gccactatcg
     5821 gcgtagttca tgcccggtat gtcggcgcag gatgtttcaa aacctgcttc gcacagttcc
     5881 gagccgtcgt caaaggtgcc attgttgcag gtgatcggcg tttcgaccgt acaaacatct
     5941 ttgtcgttcc atccggtgca ggaggcattg caggtcgcca gtgtacccgt ttcgtaactc
     6001 ttgccgggga tggatgcgca atccagagta gtgccttttt cgcataactc cccatcgtcc
     6061 tttgcgccgt tcccgcaggt ggtgcagacg atatcgagcc aaccggtgca ggaattgttg
     6121 caggatgccg tggtgccgga ggcgaaattc ttgtcgggaa caagggcgca gtctatcgtg
     6181 tcgccaagct cgcaggtttc accatcgtca atattgtcgt tcccgcattt agcaacatag
     6241 gtatcactat cgggcaggat gtcgtcttcg atgagttgct gatcgtcgtc cgggatcacg
     6301 ctttccgttt tgtcgggagc atcttcggtt gctatttcgt cttcgtcagc cgtcgtttcg
     6361 tcattcaagg cgtcgggcat ctcaacgcaa atgtcagaaa gacactgcag gccatcgttg
     6421 caggtgccgt tcgggtagca ggggccgccc tcggagccaa ccgccggagt ggtcgtgtcg
     6481 ttgtcggtcg taccgttcgg cttgttgtca tcgcatgccg cgaacatcag tacgaacaag
     6541 gcgcagaaga aaaaaaacgc gattcgcata atgaccccca tcgttctatt caccggacgc
     6601 cacattgtcg gatatagccg acatcaagtc aagataattt gggatatatc ctacagactt
     6661 tcgggcagtt gaacgctacg atgatcgcat gaacattcga ctcgaattcg ccaagcggtc
     6721 cgcgaaccca agaaacgcta cgagtgaccc ccggcgaccc taataattga caaattaagc
     6781 cggtacgcta ttgttctcca tgctggtttt cagaccaacc cacctatagg aggaattcat
     6841 gtcaggacac ggtgaaggcg gacccgtcaa gggactcccc gagaatgcct accgcgaact
     6901 caaagcgggc gaagtgtatc agccggtgat gccggcggcc gaaaatgttc aggaagtgac
     6961 gcgctggtcg cttttcatgg gtctgctgat ggcggcggtc ttctcggctg cggcggctta
     7021 ttccggcctc aagatcgggc aggtgttcga agcggccatc ccgatatcga tcatcgccgt
     7081 gggcgcctcg gcggcgctcc gcaagaagaa tgtgctcggc cagaatgtca tcatccagtc
     7141 gatcggcgcc gcctcgggcg tcatcgtcgc gggcgccgtg ttcacgatcc ccggcatcta
     7201 catcctgc
//
LOCUS       DAOQUE010000084         7204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_11432_length_7204_cov_4.381032, whole genome shotgun sequence.
ACCESSION   DAOQUE010000084 DAOQUE010000000
VERSION     DAOQUE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11432_length_7204_cov_4.381032"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1809)
                     /locus_tag="PKH10_03850"
     CDS             complement(<1..1809)
                     /locus_tag="PKH10_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNT27294.1"
                     /translation="MIFQELTDIFDHYRSDKDIFHDLMPFKVQEVLLVATLYDAFILE
                     RDGQLSEQIFGEYYQLNLSSAPRVTSAYSPDDAIRKLEQRKFDLVILMVGLDVRVPLA
                     LATEIKKRRDLPVVLLFNNSANLHQCPIDSPDLRDIDRIFVWNGYSKIFLAITKYIED
                     RMNVVNDTGLGMVPVILLIEDSIRFYSRYLPFLYSEVMRQTQRLIADENLDEMNKLLR
                     MRARPKILLATNYEEALQIFTQYRKALLAVISDVRFPREGTIDPQAGLTFCRSVKEEL
                     PDLPILIQSSESSNEPKAHLLGAGFINKNSETLAFDIRNFLMDRLGFGNFIFRDLQGR
                     EIGSAANMEELEHLIRTIPDESLLYHAARNHFSAWLTARGEMLFARIMRSAHAADFPS
                     PDGLRAFILSLFERVRAKKVKGRVINFDESVFGGASYIVRFANGSLGGKGRGIAFINN
                     LVEHIDLSKHIPDLPIRIPTTAVIGIDEYDRMIDAPALRNVIFRDTDHEHVKDLFLGT
                     ALSLDLRKKLKTFLSMVTCPLAVRSSGLFEDMLLQPFAGIYSTYIIPNNHPDLNVRFK
                     QLMDAIRLVYASVFSPRSRAYFDAVGYKIEEERMAVI"
     gene            complement(1806..3074)
                     /gene="hisS"
                     /locus_tag="PKH10_03855"
     CDS             complement(1806..3074)
                     /gene="hisS"
                     /locus_tag="PKH10_03855"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNT27295.1"
                     /translation="MSRYTAVKGMNDILPPESLRWSRMEEDLRLLATRLNYQEIRTPV
                     VESLELFQRGLGETSDVVQKEMYVFEDKNGNKLALKPEGTAGVVRAFIEHSIGIEKPG
                     PHKLFYIGPNFRYEKPQKGRYRQFHQFGLEIFGDPTPEMDAELIYAASMILDHFKVKG
                     EIRVNSIGCPACRPAYREKLVEYFRPKADELCGDCRRRIDSNPLRLIDCKVDAPRERW
                     RDIPIITDHLCPQCASDFERLKKALEIFHVPFVVDPFIVRGLDYYVKTAFEITADTGG
                     TQNAIAGGGRYDNLVAETGGPATPATGFAIGLERLLSVVPEDFYAPQPLAMGFALFDG
                     GIPALLHLTKHLSHHPVRYMAEYTPRKFKNALQKANKLGARHIFIFGEDEVKNSQVLY
                     KDLATGDQKTFGQHETMTIVSMLMDKEIEK"
     gene            3243..4064
                     /locus_tag="PKH10_03860"
     CDS             3243..4064
                     /locus_tag="PKH10_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27296.1"
                     /translation="MRPFLMILCLPVILWSCAENTPAPADADTATVDADTVVTDEAIS
                     DEAAPDEDELLPDDACVNLAVEDAFLEQIGGYDAYLAFRAVGMINEADVTTPVKLFQS
                     KFVALLPEHTGITLDQAVGFILEGERDEKAVLEMTVLADPGTKIYGSLTVVVLSKEWL
                     VSHKDDLFVDPVIEEAPAVQVMSVEAASQTVVRKCVVAMSAFDPEHPDLPAPGRMQVS
                     VCDNVDFSAEENLRIGVNAKLTEDVTTILALLGLTEAEELCSCADAATNEPVDCP"
     gene            complement(4061..5899)
                     /locus_tag="PKH10_03865"
     CDS             complement(4061..5899)
                     /locus_tag="PKH10_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNT27297.1"
                     /translation="MKKTCALILILFFALSCDDAKQTRPDADATDDTAVNDDVLDELL
                     TEDAPDEDTVPFDGDFEQIVVIPKEGIELTATVDITRWQERLFTVIEKLSFPAPADGN
                     TVKLFGEKMSIAQASVPFENDQHTILFFPGEFKAGDPLVIEATFSFEQSSSMGLRVWE
                     DDATDEKVVGPFTEPYFTPYWLIVPQSPFKTDKDNDANVPVEKVKLSVVVPGDEWQVM
                     GPGVTGTREGDVVTFTMDTPMPFYALSFAASPDHTYFSAGKSASGVEVIGAGFAREIE
                     NLKQIYPAGVATVDWMEEHLGPYEFGDKVGLVSIPDFGGGMEHVGVIYMGTDVLADYD
                     SGLFVTVHEIVHNWWGDNVRFADWPDFWVAEGFDEWTTNYNLMAVLEDADAFAARRAQ
                     YRSVAALLCSSADAVPLKFDADKDFMTLDMQIQTSYYYGAAFLEMVNKRLAGFNGQSL
                     LPLLKLWFEEKHLTTVTTEDFLDFLIAHTSDALNSTTYWNSLFDDWVYSAPCPTLKVS
                     NYTEDCYGFTLTHGGKGPDLFNLSVQFLEEDGSVIVEKSVELKKGESEIVGGYCDGPA
                     PARILIDPEWSYVFSLDTTGWKGPEIGYIDTAPAKRRYPLSPKERY"
     gene            complement(5917..6918)
                     /locus_tag="PKH10_03870"
     CDS             complement(5917..6918)
                     /locus_tag="PKH10_03870"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT27298.1"
                     /translation="MYVVTGASGHVGNTLIRMFVEQGKADQVVAMIHCNKEPESLKGL
                     PVKRVCGDICDYQTLVPLFKGADVVFHCAALISIVPGMYKKLNEVNYDGTLNVMRACR
                     EAGVRRLVHVASIEAIGDLCTGRPVTEADGFNPEHAMIEYGVTKANAALKVVEEAQKG
                     LDVVIVSPVGVVGPYDFKPSQMGKMVLDYAAKKLPAYPDGGFDFVDSRDVAKALLAAA
                     EKGHTGENYLVTGGHLSIPEMMAMLEEVSGIRKPLFKLPHWVMTVAALGSEALFHLFG
                     GNPIVTRGSLRILKSRLKVCGEKTRRELGVEPTPLKKTFADQIAFFREHGMLPPETE"
     gene            complement(6947..>7204)
                     /locus_tag="PKH10_03875"
     CDS             complement(6947..>7204)
                     /locus_tag="PKH10_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNT27299.1"
                     /translation="IQISTRPADFIETATAREDDEEQDKLWDEVIEYLKKNRSCSASM
                     LQRKFKIGYNRASRLVDQLEAAGIVGPGDGAKPRDVLLPED"
BASE COUNT         1327 a         2046 c         2338 g         1493 t
ORIGIN      
        1 gatgaccgcc atccgctctt cctctatctt gtagccgacc gcgtcgaaat aggcccgcga
       61 ccgcggcgag aagaccgacg cgtacacgag ccggatggcg tccatgagtt gcttgaagcg
      121 gacattgaga tcggggtggt tgttcgggat gatgtaggtg ctgtaaatgc cggcgaacgg
      181 ctgcaggagc atgtcctcga acaggcccga cgagcggacc gcgagcgggc aggtgaccat
      241 cgagaggaag gtcttcagtt tcttgcgcag gtcgagcgac agcgcggtgc cgaggaacag
      301 gtccttgaca tgttcatggt cggtgtcgcg gaatatgaca ttccgcaggg ccggcgcatc
      361 tatcatgcga tcgtattcgt cgatgccgat gacggcggtc gtcgggatgc ggatcggcag
      421 gtcggggatg tgtttcgaga ggtctatgtg ttcgacgagg ttattgatga aggcgatgcc
      481 gcgccctttg ccgccgagcg agccgttggc gaagcggacg atgtaactgg cgccgccgaa
      541 caccgattcg tcgaaattta tcacgcggcc cttgaccttt ttggcgcgca cccgttcgaa
      601 cagcgacagg atgaaggcgc gcaatccgtc gggcgaaggg aagtcggcgg cgtgggccga
      661 ccgcatgatg cgggcgaaca gcatctcccc gcgcgcggtg agccacgcgc tgaaatggtt
      721 gcgggcggca tggtagagca gcgattcgtc gggtatcgtg cggatgaggt gctcgagttc
      781 ctccatgttc gcggcgctgc cgatctctcg cccctgcagg tcgcggaata tgaagttgcc
      841 gaatccgagc cggtccatca ggaagttgcg gatgtcgaac gcgagcgttt ccgagttctt
      901 gttgatgaac ccggcgccga gcaggtgcgc cttcggctcg ttcgacgact cggaactctg
      961 gatgagtatc ggcagatcgg gcagttcctc cttcaccgac cggcagaagg tgagacccgc
     1021 ctgcgggtcg atcgttccct cgcgcgggaa ccggacatcg gatatgaccg cgagcaaggc
     1081 cttgcggtac tgtgtgaaga tctggagcgc ctcttcgtag ttggtggcca gcagtatctt
     1141 ggggcgcgcc cgcatccgca acagtttgtt catctcgtcg aggttctcgt cggcgatgag
     1201 ccgctgggtc tgccgcatca cctcggagta gagaaagggg agatagcgtg aatagaaccg
     1261 gatgctgtcc tcgatgagca gtatcaccgg caccatgccg agtccggtgt cgttgacgac
     1321 attcatccgg tcctcgatgt acttggtgat ggcgaggaat atcttggaat agccgttcca
     1381 gacgaatatg cggtcaatgt cgcgcaggtc gggcgagtcg atggggcact ggtggagatt
     1441 cgccgagttg ttgaacagca ggaccaccgg caggtcgcgc cgtttcttta tctcggtggc
     1501 gagtgcgagc gggacgcgga catcgagccc gaccatgaga atgacgaggt cgaatttgcg
     1561 ctgttccagt ttgcgtatcg cgtcgtcggg gctgtaggcg ctcgtgacgc gtggcgccga
     1621 agagaggttc aactggtagt actcgccgaa tatctgctcc gacagttggc catcgcgttc
     1681 gaggatgaag gcgtcgtaga gcgtcgcgac gagcaggacc tcctgcacct tgaacggcat
     1741 gaggtcgtgg aatatgtcct tgtcggaccg gtagtggtcg aatatgtcgg tgagttcctg
     1801 aaagatcatt tctctatctc tttatccatc agcatcgaga cgatggtcat cgtttcgtgc
     1861 tggccgaagg tcttctggtc gcccgtcgcg aggtccttgt agagcacctg actattcttc
     1921 acctcgtcct cgccgaagat gaagatatgg cgcgcgccca gtttgttggc cttctgcagg
     1981 gcgttcttga atttgcgcgg cgtatattcg gccatgtagc ggaccggatg gtgcgagaga
     2041 tgtttggtga gatggaggag tgccgggatg ccgccgtcga agagggcgaa acccatcgcg
     2101 agcggctggg gtgcgtagaa gtcttccggc acgaccgaca gaagccgttc aaggccgatg
     2161 gcgaaaccgg tggcgggggt cgccgggccg cccgtctcgg cgacgaggtt gtcgtagcgg
     2221 ccgccgcccg cgatggcgtt ctgcgtgccg ccggtgtcgg ccgttatctc gaaggcggtc
     2281 ttcacatagt agtcgagccc gcggacgatg aagggatcga ccacgaacgg gacatggaat
     2341 atctcaagcg cttttttcag ccgctcaaag tccgacgcgc actgcgggca gaggtgatcg
     2401 gtgatgatcg ggatgtcgcg ccatcgttca cgcggggcat cgaccttgca gtcgatgagc
     2461 cgcaggggat tgctgtcgat gcgccggcgg cagtcgccac agagttcgtc ggctttcggg
     2521 cggaagtatt cgacgagttt ctcgcgatac gcggggcggc aggcgggaca gccgatggag
     2581 ttcacccgta tctcgccctt caccttgaag tgatcgagta tcatcgaggc ggcgtagatc
     2641 agttcggcgt ccatctcggg ggtcgggtcg ccgaatatct caagcccgaa ctggtggaac
     2701 tggcggtaac gccctttctg cggtttctcg tagcggaagt ttgggccgat gtagaacagt
     2761 ttgtgcgggc ccggcttctc gatgccgatg ctgtgctcga tgaaggcgcg caccaccccg
     2821 gcggtccctt cgggcttgag cgccagtttg ttgccgttct tatcctcgaa gacatacatc
     2881 tccttctgca ccacatcgct cgtttcgccc agcccgcgct ggaacaattc cagcgattcg
     2941 accaccgggg tccgtatctc ctggtagttc aggcgcgtgg ccagcaggcg caggtcctct
     3001 tccatccggc tccagcggag cgattcggga ggcaggatgt cgttcatgcc cttgacggcg
     3061 gtgtagcggc tcatcggctc ctccatggtg ggaacggaga ggaatctagc atggaaaaaa
     3121 gaaagttaca agaaatcggc aggtcgtcgc tcgcgacggg gttgcttttt tgacgggagc
     3181 agggtatcgt tcgttcgtcc cgataaaaaa cggcgcgtta cgaacacccg gaggagggaa
     3241 ccatgcgacc ctttctgatg atcttgtgtc tacctgtcat tctatggtct tgtgccgaaa
     3301 atacccctgc gcccgcggat gccgacacgg cgaccgttga cgctgacacc gtcgtgaccg
     3361 acgaggcgat ctccgacgag gcggcgcctg acgaggatga acttctcccg gatgatgcct
     3421 gcgtgaacct cgccgtggag gatgcgttcc tcgaacagat cgggggctac gacgcctatc
     3481 tcgcattccg cgcggtcggc atgatcaacg aggccgatgt cactacgccg gtgaaactgt
     3541 tccagtcaaa gttcgtggcg ctcctgccgg agcatacggg gataacgctc gatcaggcgg
     3601 tcggcttcat tctggagggg gagcgggacg aaaaggcggt gctcgagatg accgtgcttg
     3661 ccgaccccgg tacgaagatc tacggcagtc tcacggtcgt cgttctgtca aaggaatggc
     3721 tggtctcaca taaggatgat ctgttcgttg atccggtgat cgaggaggcg ccggcggtgc
     3781 aggtgatgag cgtggaggcg gcatcccaga ccgtcgtcag aaaatgcgtc gtcgccatga
     3841 gcgcgttcga tccggagcat cccgaccttc ccgcgccggg gaggatgcag gtgtcggtct
     3901 gtgacaatgt cgatttctcg gcggaggaga acctgcgcat cggggtcaat gccaaactta
     3961 ccgaggatgt cactacgata ctcgcgcttc tcggcctcac cgaagcggag gaactctgct
     4021 catgcgccga tgcagcgacc aacgaaccgg tcgactgtcc ctaatatcgt tccttcgggg
     4081 atagcggata tcgtcgcttg gcgggcgccg tgtcgatgta cccgatctcg gggcccttcc
     4141 agccggtcgt gtcgagcgag aacacatagg accattcggg atcgatgagt atccgcgccg
     4201 gagccggacc gtcgcaatag cctcctacga tctcactttc cccttttttg agttcgacgc
     4261 ttttttcgac gatgacagag ccgtcctctt ccaagaactg gaccgacagg ttgaagagat
     4321 cgggaccttt gccgccatgg gtcaaggtga acccgtagca gtcctccgta tagttcgaga
     4381 ccttgagcgt cgggcacggt gcgctgtaga cccagtcgtc gaagagagag ttccaatagg
     4441 tggtggagtt gagtgcgtcg gaggtgtggg cgatgaggaa atcgaggaag tcctcggtgg
     4501 tgacggtggt caggtgtttc tcttcgaacc agagtttcaa aagcgggagg agcgactgtc
     4561 cgttgaagcc ggcgagccgc ttgttgacca tctcgaggaa cgccgcgccg tagtaatagg
     4621 aggtctgtat ctgcatgtcg agcgtcataa agtccttgtc ggcgtcgaac ttgagcggca
     4681 ccgcgtcggc cgacgagcaa aggagcgccg cgaccgaccg gtactgggcg cgccgcgcgg
     4741 cgaaggcgtc ggcatcttcg aggaccgcca tgaggttgta gttggtcgtc cattcgtcga
     4801 acccttcggc cacccagaaa tcgggccagt cggcgaaccg gacattgtcg ccccaccagt
     4861 tgtggacgat ctcgtggacc gtgacgaaca ggcccgaatc gtagtcggcg agcacatcgg
     4921 tccccatgta gatgaccccg acatgctcca tgccgccgcc gaagtcgggt atggagacga
     4981 gccccacctt atcgccgaac tcgtacgggc cgaggtgctc ttccatccaa tcgacggtcg
     5041 cgacgccggc ggggtatatc tgtttgagat tctcgatctc ccgcgcgaac ccggcgccga
     5101 tcacctcgac gcccgacgcg cttttcccgg cagagaagta ggtgtggtcg ggcgaggcgg
     5161 cgaacgagag ggcgtaaaag ggcatcgggg tgtccatcgt gaaggtgacg acatcaccct
     5221 cccgcgtgcc ggtgaccccg gggcccatca cctgccactc atcgccgggg acgacgaccg
     5281 agagtttaac cttctcgacc gggacatttg cgtcattatc cttgtcggtc ttgaaggggc
     5341 tctgcggcac gatgagccag taaggggtga agtagggttc ggtgaaggga ccgaccactt
     5401 tctcgtccgt cgcgtcgtcc tcccataccc ggagccccat cgaggatgac tgctcgaacg
     5461 agaaggtcgc ctcgatgacg agcggatcgc ccgccttgaa ttcgccgggg aagaacaaga
     5521 tggtgtgctg gtcgttctcg aaggggaccg aggcctgcgc gatggacatc ttctcgccga
     5581 agagtttgac ggtgttgcca tcggcggggg cggggaacga gagtttctcg atgaccgtga
     5641 agagtcgctc ctgccaccgg gtgatgtcga ccgtcgcggt gagttcgatg ccctccttcg
     5701 gtatcacgac gatctgctcg aaatcgccgt cgaaagggac ggtgtcctcg tcgggcgcgt
     5761 cttcggtcaa taactcatcg aggacatcgt cgttcacggc ggtgtcatcg gtcgcatcgg
     5821 cgtcgggccg cgtctgtttc gcgtcgtcgc aggacagcgc gaaaaaaagg atcaggatga
     5881 gggcgcaggt ctttttcatg acgggtctcc ggcggttcat tcagtttcgg gcggcagcat
     5941 gccgtgctcg cggaagaacg ctatctggtc agcaaaggtc tttttaagcg gggtcggctc
     6001 gaccccgagt tcccgtcgcg tcttctcgcc gcacaccttg aggcggctct tgaggatgcg
     6061 cagagagccg cgtgtcacga tgggattccc gccgaacagg tgaaaaagcg cttcgctgcc
     6121 gagcgccgcg acggtcatga cccagtgggg cagtttgaag agcggtttgc ggatgccgct
     6181 cacctcctcg agcatcgcca tcatctccgg tatcgagaga tggccgccgg tcacgaggta
     6241 gttctcgccg gtgtgtccct tttcggccgc cgcgagaagc gccttcgcca catcgcggga
     6301 atcgacgaaa tcaaagccgc cgtcgggata ggcgggcagt ttttttgccg cgtaatccag
     6361 taccattttc cccatctggg acggcttgaa gtcatagggg ccgacgacgc cgaccggcga
     6421 gacgatgacc acatcgagcc ccttctgcgc ctcttcgacc accttgagcg ccgcgttggc
     6481 cttggtgacg ccgtactcta tcatcgcatg ttcggggttg aacccgtccg cctcggtgac
     6541 cggccgaccg gtgcacagat cgccgatcgc ctctatcgac gcgacatgga cgagccgccg
     6601 cacccccgcc tcgcggcagg cacgcatgac attcagcgtc ccgtcgtaat tgacctcgtt
     6661 cagtttcttg tacatgccgg ggacgatgct gatgagcgcg gcacagtgga acaccacatc
     6721 ggctcccttg aacagcggca cgagcgtctg gtaatcgcag atgtcgccgc agacccgttt
     6781 caccggtagg cccttcagcg attccggctc tttgttgcag tggatcatcg cgaccacttg
     6841 atccgccttt ccctgttcga cgaacatcct gatgagcgtg ttgcctacat gtcccgatgc
     6901 gccggtgacg acatacatga gcgttctccg tacgaaaggt gaggggttaa tcttccggca
     6961 acagcacatc gcgcggcttg gccccgtcgc cggggccgac gatgccggcc gcctccaact
     7021 gatcgacgag ccgcgaggcg cggttgtagc ctatcttgaa cttgcgctga agcatcgagg
     7081 cggaacagga gcggttcttt ttcaggtatt cgatgacctc gtcccacaat ttgtcctgct
     7141 cctcgtcgtc ctcacgggcc gtagccgtct cgatgaagtc ggcgggacgg gtcgatatct
     7201 ggat
//
LOCUS       DAOQUE010000085         7176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_11497_length_7176_cov_3.856481, whole genome shotgun sequence.
ACCESSION   DAOQUE010000085 DAOQUE010000000
VERSION     DAOQUE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11497_length_7176_cov_3.856481"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PKH10_03880"
     CDS             complement(<1..593)
                     /locus_tag="PKH10_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27300.1"
                     /translation="MKQALRRTDIPVILLHNIDPRWEPPAVEEVLKDVKTMSDSLMAE
                     GHPVIDLPVRSPDIEKRLKPYDPEKHIVLNWCEEIPTQPKSEVDVVALLEELSYTYTG
                     SSSEVLALSWDKMKVKRILQEKGVSTPRGEIVSSEETDRWDLFPAIVKPSREHGSAGV
                     TADAVVMDRTALRTRIAYIEKKYKQPAIVEDFIEGRE"
     gene            927..1253
                     /locus_tag="PKH10_03885"
     CDS             927..1253
                     /locus_tag="PKH10_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012857891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNT27301.1"
                     /translation="MLAAILGGVTAERVLVYLSRFGEGYARGIARHFGIAPSPVQKQL
                     AKFEEAGILASVEKGRTLVYLWNPRYPFLAELQALLVKAYDYLTPEEKERYTLRTRPR
                     RRGKPA"
     gene            1250..1774
                     /locus_tag="PKH10_03890"
     CDS             1250..1774
                     /locus_tag="PKH10_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNT27302.1"
                     /translation="MKITAKTTIKDLALIVCGTLKKNGIDAVLTGGAVVSIYTGNRYR
                     SNDLDFISSASMKAIEAALAAIGFTKNKGRYFTHHDTELFVEFPSPPLSVGDRPVREF
                     NEITTRSGYLKLLTPTHCVMDRLAAFYFWNDRQSLEQAVMVARAQSVDMKEIGRWSKV
                     EKMTAKFEEFKAAV"
     gene            complement(1835..2128)
                     /locus_tag="PKH10_03895"
     CDS             complement(1835..2128)
                     /locus_tag="PKH10_03895"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HNT27303.1"
                     /translation="MDTRIKSDDVFGGAEIEFPVKFDLKLFVNGALPEKDSIAAVTAL
                     CGTLAVPAGKASVRPSQGGKYYCLTIPVTVDSKDIFDKLFAAVRDIPGVKMVL"
     gene            complement(2141..2872)
                     /locus_tag="PKH10_03900"
     CDS             complement(2141..2872)
                     /locus_tag="PKH10_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27304.1"
                     /translation="MTKWLPLLLICTFLAACDGTTVDDEFPDENGSTSDQNGSTSDEN
                     GSTTDGATNPDDPAYDNTYPDDQPWPDGQVADDEQPDNGETPDEGGEEDDPLRLYKSG
                     SRIRARFGETPDGAKQFMGWYDNQIGADCAFYQATDGQYRCIPTPILYANYYADSSCS
                     TPLFMVTVGCDAVASWGLISDTCSRPARVFQRGSVYGGSSGYVKSGTNCVSLDFSSYA
                     GIYTFYNAGQEYPLSSFQTMNISTE"
     gene            3028..4095
                     /locus_tag="PKH10_03905"
     CDS             3028..4095
                     /locus_tag="PKH10_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27305.1"
                     /translation="MLIPFDRNVLYVQKIPGRYLFSLLNDLFRHEKDISGYLFCVAPG
                     LVGYLFFKNSKMLSAAAFQFPGGAGMLQPISFRHFVEHEDIDIYANVVEEATILENLH
                     RFFASACLLHAPVTVVDAGKCLAALSEDSFSGMAGFQQGMVMNMAFYDRGRFRYFLYY
                     HPDTKSYAYENDEAVFTRYVELLPKLTPVLTTRDFRVKGTADGINDIVVSQQKDLIVN
                     LLLGYFDIFDILLQMLNDRQPPAQVAQSVNLMFDQLREKYDPLYRNIGFSPDTRSVNW
                     MDILDDRKYVPLQYRFEHYHLYIDEIWKRLLNLLVAAGGDAAVAEIREKITKYLSLVD
                     KDEREIKRMLYRLEQQCEKKG"
     gene            4127..5056
                     /locus_tag="PKH10_03910"
     CDS             4127..5056
                     /locus_tag="PKH10_03910"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNT27306.1"
                     /translation="MGLKIIVENDRSELSVHEFNAGLITAGRAPDNDLVLPERNVSRH
                     HFQLELLDKRIVIEDRGSYNASFVNGKEIAGKVEIAVGDIITVGDYSIYLDQDDEEEG
                     GASLSREIDASPKTAETDLLMAKSGVIGGRVFAIKGAETVIGAHAGADVYLGRPDIPD
                     VYAKIIFDGNIFLLIRGVRDKDHPLIVNGMKIESIDLRHGDEVQVADFLFDYIEKGQE
                     YNPYQYLIAADEERRRRLREDLDEKKIRSLRDEDEVTEVTKRAEKTRQVVRSRRSLLI
                     LLLLSAAVLLGVTILAKSGYLPIDLPSFFQSLF"
     gene            5075..6166
                     /gene="mtnA"
                     /locus_tag="PKH10_03915"
     CDS             5075..6166
                     /gene="mtnA"
                     /locus_tag="PKH10_03915"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNT27307.1"
                     /translation="MDGNTGLRAAYWEGDEYVILDQLELPGRVSHLRLTSPAEVREAI
                     VSMRLRGAPLIGAAASAGAALAFRGADAPLPRPVVASVCASLASARPTAVNLTVVLDE
                     MLAFYDAEVAGCASGAAQFALMQRRSLELHWRDRDRNRRMARHGADRLRRLRPGRKLR
                     VLTHCNTGALATCGYGTALGVIRALHEDGALEMVWVDETRPYLQGARLTAFECAEEGM
                     PHTVITDSTAAFLMARGLIDAVIVGADRMARTGDVANKIGTYGLAVSARHHGIPFLVA
                     LPVESFDPGAASGQAIVIEERSDDELLVCNGRRIAPVGTRGLHLGFDVTPGALVSAVV
                     TEHGVLEGPIQEEHLAVFMDHRARGGDRP"
     gene            6120..>7176
                     /locus_tag="PKH10_03920"
     CDS             6120..>7176
                     /locus_tag="PKH10_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27308.1"
                     /translation="MRFSWIIGLAAAIALETACASFDGATHDRAMVSQRTARELYLGD
                     WPKAVEAAQAAVDAAPDEAEVASFIVGEALYLAGDHEEAFPHLLRALRGRDPIAALAA
                     LYIIRDLYVSRRIASDIAATHPACANDLCRAVIAAEAWEWARVMQRPWDEHRLRTELH
                     APHAFRLFRAAGASAFRDLSGTTPEEEAYLAGAPFPTTDLRVVTFEEAGPSLNLRNYF
                     SPIGNAVFFAAGELSLPEDGPVTILPYITVPFRLYVDGKPVMERDADRFSLGLVAASA
                     RLTLAKGKHSVLLKLAPYLSEDAIFSIAFHNDGAALPETDADDPSTPKEGDDLFALHL
                     RLMIRDLLYGDVGMPQHL"
BASE COUNT         1306 a         2228 c         2239 g         1403 t
ORIGIN      
        1 atctcgcgcc cctcgatgaa atcctcgacg atcgccggtt gcttgtattt cttctcgatg
       61 taggcgatgc gggtgcgcag cgccgtgcgg tccatcacca ccgcgtcggc cgtcacgccg
      121 gcgctcccgt gctcgcgcga cggcttgacg atcgcgggga acaggtccca ccggtcggtc
      181 tcttcggagg atacgatctc gccgcgcggg gtggatacgc ccttttcctg aagtatccgc
      241 ttgaccttca tcttatccca cgagagcgcg agcacctcgg aggaagaccc ggtgtaggtg
      301 taggagagtt cttcgagcaa cgccaccaca tcgacctcgc tcttgggctg ggtcggtatc
      361 tcctcgcacc agttgaggac gatgtgcttc tcgggatcgt agggcttcag ccgcttctcg
      421 atgtcgggcg accgcaccgg cagatcgatg accggatgac cctcggccat cagcgagtcg
      481 ctcatggtct tcacatcctt gagcacctct tcgacggcgg gcggttccca gcgcgggtcg
      541 atgttgtgaa gaagtatgac gggtatatcg gtccggcgta acgcctgttt catgcagacc
      601 cccgcttgtc cgtaaagcca gtcgcaaggc cgggctcagc gtaacacgcg gaggttctac
      661 cgatgatctg tgttatgagt tttgaactgt gagcgtcatt cctactcata agaaaaacat
      721 ataaggttct ttttcctcat gtcaaatttt aatccgtatt ttttgaaaaa aaattcactc
      781 gatactctcc ggcgtctttt tttcgccgtt cccgacccct cctttttctg ctttccgctg
      841 gcgccgggag gcgcttcttt ttttgagctt gactttgcaa aatagaccgt tattatagtc
      901 catgtgaact actttggagg tcttccatgc ttgcggcgat cttaggcggc gtcacggcgg
      961 aacgcgttct tgtctacctc tcccgcttcg gcgagggcta cgcgcgcggc atcgcccggc
     1021 acttcggtat cgcgccgagt ccggtgcaga aacagttggc gaagttcgag gaggcgggca
     1081 tactcgcgag cgtcgaaaaa ggacggacgc tcgtctatct ctggaacccg cgctacccgt
     1141 tcctcgccga gttgcaggcg ctcctcgtca aggcgtacga ttacctgacg ccggaggaga
     1201 aggaacgcta cacactcagg acgcgaccgc gacgcagggg gaaaccggca tgaagataac
     1261 ggcgaagacc accataaagg atctggcgct catcgtctgc gggacgctca agaaaaatgg
     1321 catcgacgcg gtgctgaccg gcggcgcggt ggtgtccatc tacaccggga accgctaccg
     1381 ctcaaacgac ctcgatttca tctcgtcggc ctcgatgaag gctatcgaag cggcgctcgc
     1441 cgccatcggc ttcacgaaga acaagggacg ctacttcacg caccacgaca ccgaactgtt
     1501 cgtcgagttc ccctcgccgc ctctctcggt gggggatagg ccggtgcggg agttcaacga
     1561 gataacgacg cggagcggct atctgaaact gctgacgccg acccactgcg tcatggaccg
     1621 gctggctgct ttctatttct ggaacgaccg gcaatcgttg gagcaggcgg tcatggtggc
     1681 gcgggcacag tcggtggaca tgaaagagat agggcgctgg tcgaaggtcg agaagatgac
     1741 ggcgaagttc gaggagttca aggcggcggt gtgagaaatt tgtgcgggtt tttgtggggg
     1801 aagatcaaat cataagtcgt gggaagaggt gtgttcagag caccatcttg acgccgggga
     1861 tatcgcggac ggcggcgaag agtttgtcga agatatcttt tgaatcgacc gtcaccggga
     1921 tggtgaggca gtagtatttg cctccctgcg aggggcgtac cgatgccttt cccgccggga
     1981 ccgcgagcgt tccgcagagc gccgttaccg ccgctataga atctttttcg ggcagagcgc
     2041 cgttcacgaa gagtttcaga tcgaacttca ccgggaactc gatctcggcg ccgccgaaga
     2101 catcgtcgct ttttatccgc gtatccatga gcggaccgcc ttattcggtc gagatgttca
     2161 tcgtctggaa actcgacagc gggtattcct gcccggcgtt atagaaggtg tagatgccgg
     2221 cgtaggacga gaagtccagg gagacgcagt tggttcccga cttcacatag cccgagctcc
     2281 cgccatagac cgaaccgcgt tggaacaccc gagccggtcg cgagcatgtg tcggagatca
     2341 atccccacga cgcgaccgcg tcgcacccca cggtgaccat gaacagcggc gtcgaacagg
     2401 aggagtcggc gtaataattt gcgtagagaa tgggcgtggg gatacagcga tactggccgt
     2461 cggtggcctg atagaatgcg cagtcggcgc ctatctggtt gtcgtaccag cccatgaact
     2521 gcttcgcgcc gtcgggcgtt tcgccgaatc gggcgcggat gcgggatccc gacttgtaga
     2581 gccgcagggg gtcgtcctcc tcgccgccct cgtcgggtgt ctcgccgtta tcgggttgct
     2641 catcgtcggc gacctgtccg tcgggccacg gctgatcgtc gggatacgtg ttgtcgtacg
     2701 ccggatcgtc ggggttcgtc gcgccgtcgg tcgtcgagcc gttctcatcg ctcgtcgagc
     2761 cgttctggtc gctcgtcgag ccgttctcgt cggggaattc atcgtccacg gtcgtgccgt
     2821 cgcaggcggc cagaaaggtg cagattaaaa gcaacggaag ccacttcgtc atggtatcct
     2881 ccctcggtga aagacggtgg gagcatactc cgcccgccgc caataggcaa ggttaccccc
     2941 tccctcgcgc gcccgaacat ttaattttac aaaaggcgac gaatttagta taaaggccag
     3001 ccgaccgact tttttgagag agggtcaatg ctcatcccgt tcgaccggaa tgtactctat
     3061 gtgcagaaga taccgggccg ctatctcttt tcgctcctca acgatctgtt ccgccacgag
     3121 aaggacatat cgggctacct gttctgcgtc gcgccggggc ttgtcggcta tctgttcttc
     3181 aagaacagca agatgctgag cgccgcggcg ttccagttcc ccggcggcgc cggcatgctt
     3241 cagccgatat cgttccgcca cttcgtcgaa catgaggata tcgacatcta cgccaatgtg
     3301 gtcgaggagg cgacgatact cgagaacctc caccgtttct tcgcgtcggc ctgtctcctg
     3361 cacgcgccgg tcaccgtggt cgacgccggg aaatgtcttg cggcgctttc cgaggacagt
     3421 ttctcgggga tggcgggttt tcagcagggg atggtgatga acatggcctt ttatgaccgc
     3481 ggccggttcc gttatttcct gtactaccac cccgacacca agtcctacgc ctacgagaac
     3541 gacgaggcgg tgttcacccg ctatgtcgaa ctcttgccga aactcacgcc ggtgctcacc
     3601 acgcgcgatt tccgcgtcaa ggggaccgcc gacggcatca acgacatcgt ggtctcccag
     3661 cagaaggatc tcatcgtgaa cctcctgctc ggctatttcg acatcttcga catcctgctc
     3721 cagatgctca acgaccgcca gccgccggcg caggtcgccc agtcggtcaa cctgatgttc
     3781 gaccagttgc gcgagaaata cgacccgctg taccgcaaca tcggcttctc gcccgatacg
     3841 cggtcggtca actggatgga catactcgac gaccgcaagt atgtgccgct ccagtaccgg
     3901 ttcgaacact accatctcta catcgacgag atatggaaac ggctcctcaa tctgctcgtc
     3961 gccgccgggg gcgacgccgc cgttgccgag atccgcgaga agataacgaa gtacctgtcg
     4021 ctcgtcgata aggacgagcg cgagataaag aggatgctgt accggctcga acagcagtgc
     4081 gagaagaaag gataatgcgg ataccccgat aaggagaaca ggcgtcatgg ggctcaagat
     4141 catcgtcgag aacgatcgta gcgaactgtc cgtgcacgaa ttcaacgccg ggctcatcac
     4201 ggccggccgc gcgcccgaca acgatctggt cctgccggaa cggaatgtgt cgcgccacca
     4261 tttccagttg gaactcctcg acaagcgcat cgtcatcgag gaccgcggga gttacaacgc
     4321 cagtttcgtg aacggcaagg agatagcggg taaggtcgag atagcggtcg gcgacatcat
     4381 cacggtcggc gactacagca tctatctcga tcaggatgat gaagaggagg ggggcgcgtc
     4441 gctctcccgc gagatagacg cgtcgccgaa gaccgccgag acagacctgc tgatggccaa
     4501 gagcggcgtc atcggcggcc gtgttttcgc gatcaagggg gccgagacgg tcatcggggc
     4561 gcacgccggg gccgatgtgt atctggggcg tcccgacatc cccgatgtgt acgccaagat
     4621 catcttcgac ggcaacatct ttctgctcat ccgcggcgtt cgcgacaagg accatccgct
     4681 catcgtcaac ggcatgaaga tagagtcgat agacctgcgt cacggcgacg aggtgcaggt
     4741 ggccgatttc ctgttcgact acatcgagaa ggggcaggag tataacccgt accagtacct
     4801 gatcgcggcc gacgaggagc gccgtcgtcg gctccgcgag gacctcgacg aaaagaagat
     4861 acggtcgctc cgcgacgagg atgaggtgac cgaggtcacc aagcgggccg agaagacacg
     4921 gcaggtcgtc cggtcgcgcc ggtcgctgct catactcctg ctgctttcgg cggcggtgct
     4981 gctcggggtg acgatactcg cgaagagcgg gtacctgccg atagacctgc cctctttttt
     5041 ccagtctttg ttctgacacg ggagcgatag cccgatggac gggaacacgg gattgcgggc
     5101 cgcctactgg gaaggcgacg agtatgtgat cctcgatcag ttggaactgc ccggacgcgt
     5161 gagccacctg cggctgacga gtcccgccga ggtgcgcgag gcgatcgtat cgatgcgcct
     5221 gcgcggcgcg ccgctcatcg gcgcggccgc ctcggctggc gcggcgctcg cgttccgcgg
     5281 tgccgacgcg ccgcttccgc gcccggtcgt cgcgtcggtc tgcgcgtcgc tcgcttcggc
     5341 gcgtccgacg gcggtcaacc tgacggtggt gctggacgag atgcttgcgt tctacgacgc
     5401 cgaggtcgcc ggatgcgcct cgggcgccgc ccagttcgcg ctgatgcagc gccggtcgct
     5461 cgaactgcac tggcgcgacc gcgaccgcaa ccgccgcatg gcgcgccacg gcgccgaccg
     5521 gctccgccgc cttcggcccg gtcggaagtt gcgcgtgctg acccactgca acaccggcgc
     5581 gctcgcgacc tgcgggtatg gcacggcgct cggcgtgata cgggcgctcc acgaggacgg
     5641 cgcgctggag atggtgtggg tcgacgagac gcggccctat ctgcagggcg cccggctcac
     5701 cgcgttcgaa tgcgccgaag agggcatgcc gcacaccgtc atcaccgatt cgaccgcggc
     5761 cttcctcatg gcgcgtggct tgatcgacgc ggtgatcgtg ggcgccgacc ggatggcgcg
     5821 caccggcgat gtcgcgaaca agataggtac ctacgggctc gcggtgtcgg cgcgacacca
     5881 cggtatcccg ttcctcgtgg ccctgccggt cgaaagtttc gacccgggtg ccgcttcggg
     5941 gcaggccatc gtcatcgagg agcgttccga cgatgagttg ctcgtatgca acggtcgccg
     6001 catcgcgccg gtcggcaccc gcggcctcca tctcggtttc gatgtgacgc cgggcgccct
     6061 ggtgagcgcg gtcgtcaccg aacacggtgt cttggaagga ccaatacagg aggagcatct
     6121 tgcggttttc atggatcatc gggctcgcgg cggcgatcgc ccttgagacc gcgtgcgcct
     6181 catttgatgg cgcgacgcac gatcgagcga tggtgtccca gcggacagcg cgggaattgt
     6241 atctcggcga ttggccgaag gcggtcgagg cggcgcaggc cgccgtcgat gccgcgccgg
     6301 acgaggccga ggtcgcttcg ttcatcgtgg gcgaggcgct ctatctcgcc ggcgaccacg
     6361 aggaggcgtt cccgcatctt ctgcgtgccc tgcgcggacg cgacccgatc gccgcgctcg
     6421 ccgcgctcta catcatccgc gacctttatg tgagccgccg catcgccagc gacatcgccg
     6481 cgacgcaccc cgcgtgcgcc aacgacctct gccgcgcggt catagcggca gaagcgtggg
     6541 aatgggcccg cgtcatgcaa cggccgtggg acgagcaccg cctgcgaacg gaactgcatg
     6601 ccccgcacgc ctttcgcctg ttccgcgccg ccggcgcctc cgcattccgc gatctttccg
     6661 gtacgacgcc cgaagaggag gcctatctcg ccggcgcgcc gttcccgacg accgacctgc
     6721 gcgtcgtcac ctttgaggag gcggggccgt cgctgaacct gcgcaactat ttctcgccga
     6781 ttggcaacgc ggtctttttc gccgcgggcg aactctcgct cccggaggac ggcccggtca
     6841 ccatcctgcc ctatatcacc gtgccgttcc ggctctatgt ggacgggaag cccgtcatgg
     6901 agcgcgacgc cgaccggttc tcactgggac ttgtcgccgc ctcggcgcga ctcacgctcg
     6961 cgaagggaaa acactcggtg ctcctgaaac tggcgcccta tctttccgag gacgcgatct
     7021 tttcgatagc cttccataac gacggggcgg cgctccccga gaccgacgct gacgatccct
     7081 ccacgcccaa agagggcgac gatctgttcg cgcttcacct gcggctcatg atccgtgacc
     7141 tgctctacgg cgatgtcggc atgccccagc atctgc
//
LOCUS       DAOQUE010000086         7155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_11536_length_7155_cov_3.973380, whole genome shotgun sequence.
ACCESSION   DAOQUE010000086 DAOQUE010000000
VERSION     DAOQUE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11536_length_7155_cov_3.973380"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..917)
                     /locus_tag="PKH10_03925"
     CDS             complement(48..917)
                     /locus_tag="PKH10_03925"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT27309.1"
                     /translation="MPAERPYDLIIVGAGPAGVGAAVQAALYGARTLVIEKGCVGGRL
                     DLARRVDNFPWADEDTKRDGRHLCRRFKKWLVQTGVPTVNGTVERVAYSRGCFTLFCG
                     ENREYHASAVIVATGVEPEPWRVRGSVGAGDRVCRDWRMVPRKRGTAVAVIGGGETAF
                     DQACVLAERGHEVSVLIRGERPRAYPGLVAEAIRLGVVIVTKAAITSVRRDGRKILLR
                     AADGRSFECDHVLPAIGHRPRLPVLDRSARLKTGHGLWLAGDVREPVCRQAAIAFGDG
                     VRSAMLAWADITG"
     gene            complement(907..1458)
                     /locus_tag="PKH10_03930"
     CDS             complement(907..1458)
                     /locus_tag="PKH10_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HNT27310.1"
                     /translation="MREARYALLVIDAQNYFFDPASPAYLEGSAAILPRMNRLIAAAG
                     AAGAPVFFTLHRAPTAPGNLMAVRYKRLPRGTECEIWRGVTAPPKPILIEKEQYSAFH
                     RTGLAAQLRRRGIHDIVLCGAMTHLCVDTTARHGFMLGFKPVVVADACCSKTPAFHRA
                     ALLALGHGFAEITTTQGWLSHAR"
     gene            complement(1458..2396)
                     /locus_tag="PKH10_03935"
     CDS             complement(1458..2396)
                     /locus_tag="PKH10_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT27311.1"
                     /translation="MIVPVERDGRDRRILLKSGRPDLAEVYLAHMRGDRKYLVEFVDA
                     CAVAGGDRREKWVIIISTQFGCPAGCLMCDAGGGYLGDLTADEMLWEIRTVVEAHPEL
                     DPRACRKFKVQFARMGEPSLNEAVLDVIRRLGKEYPNCIPCVATLAVAGREAWFKELL
                     DLREGFRDLQLQFSMNTTDTALRDELMPIGKMSFDRIARYGEAFYRPGSRKPSLNFAV
                     SDSLPLDPGVVARTFDPERFVIKLTPLNPTQSARDNELVSLDGPGGDASLLDRRTEQF
                     RALGYEVVCSIGDWEENRIGSNCGQLAARSAREDIS"
     gene            complement(2454..2530)
                     /locus_tag="PKH10_03940"
     tRNA            complement(2454..2530)
                     /locus_tag="PKH10_03940"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2494..2496),aa:Met,seq:cat)
     gene            2671..3975
                     /locus_tag="PKH10_03945"
     CDS             2671..3975
                     /locus_tag="PKH10_03945"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNT27312.1"
                     /translation="MKRTTVFILAALIALPLFAAEPKKFKIAVMDVEDLSGTLDPNLL
                     KSATEYLRSKLVATGLYVVIDKTRQQEKLKGIIKDSKKESYQECFDKNCQIPLGQALA
                     ADNILRSSITFFGGMYSIATELVDLAKEATVAGLVADFDGSPKGMRDAINKIIDDMLA
                     QSKAPATVIKTAEPETLAVQFETATPGATLSVDGKEACRKIPCFVDVTKGTHSFELKA
                     AGHQTRKESVAITESRLIKWDLVANAPVVKPAPIVVQPKPQPVIVQPKPQPKIEPQPV
                     AKAEPAVAQGSSGQAPVEVKQEVPKPTEPEPEVNRPYLWWGVGTLGVAAAVFGVAWYF
                     SAEVGANKDKRDDLMRDYYSYPIAQRPEVYQKALDYEDKAKFNDTMRIVMFSVSGAAA
                     VAGTVLAIWQSDWSQSEPAPDAPKVSFYFDGSSVYAGASFSY"
     gene            3987..6965
                     /locus_tag="PKH10_03950"
     CDS             3987..6965
                     /locus_tag="PKH10_03950"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNT27313.1"
                     /translation="MNTTMTRMTIIAALAVFIASCEFVTNNDLLEQLKKDPPTGTADT
                     DSTADTDTTGTDDLLPDSDELTPDIDTVLTCGNNQLDTGEECDGNDAHCVDSPSGRTC
                     NNDTCECVAVCGNSIIEGDEECEAEDNIELCTEIDPANYSGGEAECDHETCKWIVSEC
                     VGMSLCGKENAVCGPGDDQDCTDIDPDTYVGGTATCNDDCDGWMTTDCSPKCTCGNGV
                     PESFINGCATTEECDDGNFADDDGCSSDCHWEAMVAVTDLAAERTTTEPAADIISFSW
                     TCVGPGVTGYILKKSRSPIANDADFDDAEVLHNDTISCTPGTVRTIDTEMDWYDGTWH
                     VAIRPVRNNTTGPLSNSVTVDTDGYMALAPEVGIQFGLVGYNAYLDRAVTLNNTSALV
                     DLIIDETTLPGDCSGKVTILSGEVSSAARVTVVPGNFHAVVLRYAPTETSSCTDGQFV
                     AKWLSHSDDGGATFTPGQASLPVSGNSGNTPPVFTDVYFNPNPVKTSEGTTAIMVQFE
                     DDNGLCDDGTIGNDIVEAVWDMRELGGGPREVMTNPSCSTDTHQYWYWKNIDVSSLTN
                     GIYVIPLTLTDKGDNTVRRNVSFAVYSGTILEVGTDKTYTSIAQPVIDAQAGDIVVVH
                     PGTYDSMEVANEDINPGGDAIIVYGIEGPGQTVIDLTDASHSGFELDSANSGFVIGGF
                     TVRNALTGAIWASATATTIKVTVTDCRFENNIKNTDGGAIFISGNLVDLTVAHSLFIG
                     NSAPSHTGGAIYASDVQNLLVVDTLFDGNTAQNGGAVLVEKPAQAVFDRCFFTDNTSS
                     TQGGAILIDGGAGIVELRNSIFAHNTSTYRGAAFYITNTVSTIPTVKIYNSTIADNTA
                     GTDAGGLYIERGDVTVKDTIIFANTAGTDLASQLYAVNENGVTVTVDNSVIGGAPVPV
                     IDPGNKINAPDGLWTNHQNLSDDPLFTATGNGAVRYRLQPGTPCLDVGSNGSTVPQYD
                     FLGYARQGAKNEIGAFEYLP"
BASE COUNT         1501 a         2345 c         2088 g         1221 t
ORIGIN      
        1 ccgcgtcgag atagtcttca tgcccaagat agacgaactg ccgggcttca accagtaata
       61 tccgcccacg ccagcatggc ggagcgcacc ccgtcaccga acgcgatagc cgcctgacgg
      121 cataccggct cgcggacatc gccggccagc cagagcccgt gccccgtctt gagccgggcc
      181 gaccggtcaa gcaccggcag tcgaggccgg tggccgatcg ccggaagcac atggtcgcac
      241 tcgaatgagc gaccgtcggc cgccctgagt agtatcttgc gcccatcgcg acgaacggag
      301 gttatcgccg ccttcgtgac gatcacgacg cccagccgga tcgcctcggc cacaagaccc
      361 ggataagccc gcggtcgttc cccccggata agaacggaaa cctcgtggcc acgctcggcc
      421 aaaacacagg cctgatcgaa cgctgtttcg cctccgccga tgaccgccac cgccgtcccg
      481 cgcttccggg ggaccatccg ccagtcgcga cagacgcgat cgcccgcgcc gaccgaacca
      541 cggacacgcc acggttccgg ctccacgccg gtcgccacga tgaccgccga cgcatggtat
      601 tccctgttct ccccgcaaaa gagggtgaaa cagccccggg aataggctac ccgctccacc
      661 gttccgttca ctgtcggcac cccggtctgc acaagccact ttttgaaccg gcggcagaga
      721 tgtctaccgt cccgtttcgt atcttcatca gcccacggaa agttatccac ccgccgcgcc
      781 agatcgagcc gaccgccgac acatcctttt tcgatgacga gcgtccgcgc cccgtacagc
      841 gcggcctgca ccgccgcccc taccccggca ggtcccgcgc caacgataat gaggtcatac
      901 ggtctttcag cgggcatggc tcagccatcc ctgcgtggtg gtgatctcgg cgaacccgtg
      961 cccgagcgcc agcaaggcgg cgcgatggaa cgccggggtc ttggaacagc aggcgtcggc
     1021 gacgacgacc ggtttgaagc ccagcatgaa cccgtgacgc gccgtggtat cgacgcacag
     1081 gtgggtcatc gcgccgcaca gaacgatatc gtggatgccg cgccttcgca gttgcgcggc
     1141 aagcccggta cggtggaaag ccgagtactg ttccttctct atcaggatcg gcttgggagg
     1201 ggcggttacg ccgcgccaga tctcgcactc cgtccccctc ggcaatcttt tgtagcggac
     1261 cgccatgagg ttccccggcg ccgtcggagc acgatgcaga gtgaaaaata ccggcgcgcc
     1321 cgcggcaccg gccgccgcta tcaaccggtt catccgcggg agtatggcgg cggaaccctc
     1381 aagataggcg ggggacgcgg gatcgaaaaa gtagttctgg gcatcgatga ccagcagtgc
     1441 gtagcgcgct tcccgcatca ggagatgtcc tcgcgggccg accgcgccgc cagttgaccg
     1501 cagttactgc ctatccggtt ttcctcccag tcgccgatgg agcagaccac ctcgtacccg
     1561 agcgcgcgga actgttcggt ccgcctgtcg agcagggacg cgtcaccgcc cggcccgtcg
     1621 agcgacacca gttcgttatc gcgggcgctc tgggtgggat tcagcggcgt cagtttgatg
     1681 acgaaccgtt ccggatcgaa ggtccgggcg accacgccgg ggtcaagcgg gagcgaatcg
     1741 ctgaccgcga agttcaacga cggtttccgc gatcccggtc gatagaacgc ctcgccgtac
     1801 cgggcgatcc ggtcgaacga catcttgccg atgggcatca gttcgtcgcg gagcgcggta
     1861 tcggtggtgt tcatcgagaa ctgcagttgc aggtcgcgga acccttcccg cagatcgagc
     1921 agttccttga accatgcttc ccgaccggcg acggccaagg tcgccacgca gggaatgcag
     1981 ttcggatatt ctttgcccag ccgccggatg acatcgagca ccgcttcgtt gagcgacggc
     2041 tcccccatcc gggcgaactg caccttgaac ttgcggcagg cccgcggatc aagttccggg
     2101 tgggcctcca cgacggtccg tatctcccag agcatctcgt cggcggtcag gtcgccgagg
     2161 tacccgcccc ccgcgtcgca catcagacaa ccggccggac agccgaactg cgtggagatg
     2221 atgatgaccc acttctcccg gcggtcgccc cccgcgacgg cacaggcatc cacgaactcg
     2281 accagatact tccggtcgcc ccgcatgtgg gcgagataga cctccgcgag gtcgggacgg
     2341 ccactcttca agagtatccg ccggtcacgc ccatcgcgtt cgactggaac gatcatgatc
     2401 cccccgacaa aaaatgggtc gaaccccgca gggctcaacc cgttgaaaac agatggtggc
     2461 gagggagtga ctcgaacact caaccctcgg gatatgaatc cgatgctcta accagttgag
     2521 ctacctcgcc acgaggcggt gccgataata tacgcccgga gatgtttgtc aaaaattttt
     2581 gcggtcccct catgagtgtc accagagaat ttgacaaaag agtacaagtc gataaagttt
     2641 tccatctatt cgctcttctg gaggatgtgg atgaaaagaa caaccgtttt tatccttgcg
     2701 gcactgatcg ccctgccgct cttcgcggcg gaaccgaaaa agttcaagat cgccgtcatg
     2761 gatgtcgagg acctttcggg aacgctcgac ccgaacctgc tcaagagcgc caccgagtac
     2821 ctgcgctcga aactcgtcgc gaccggcctg tatgtggtca tcgacaagac gcgccagcag
     2881 gagaaactta aagggatcat aaaggattcc aaaaaagaga gttatcagga gtgcttcgac
     2941 aagaactgcc agataccgct cgggcaggcg ctcgccgccg ataacatcct ccgctcctcc
     3001 atcaccttct tcggcggcat gtacagcatc gcgaccgaac tggtcgatct cgccaaggag
     3061 gcgacggtcg ccgggctcgt cgccgatttc gacggcagcc ccaaggggat gcgcgacgcg
     3121 ataaacaaga tcatcgacga catgctcgcg cagtcgaagg cgcccgccac ggtcatcaag
     3181 accgccgagc ccgaaacgct tgcggtccag ttcgagaccg cgacccccgg cgcgaccctt
     3241 tcggtggacg ggaaagaggc ctgtcggaag ataccctgtt tcgtcgatgt caccaagggc
     3301 acgcactcct tcgaactcaa ggcggcgggc caccagacgc gcaaggaatc ggtggcaata
     3361 accgagagcc gactcatcaa gtgggacctc gtcgcgaacg ccccggtcgt gaagccggcg
     3421 ccgatagtgg tccagccgaa gccgcaaccg gtgatagtcc aaccgaagcc gcaaccgaag
     3481 atagagccgc aaccggtcgc gaaggcggag cccgccgtcg cgcaaggctc tagcgggcag
     3541 gccccggtcg aggtgaagca ggaggtgccc aagccgacgg agcccgaacc ggaggtgaac
     3601 cgtccctacc tgtggtgggg cgtcgggacg ctcggcgtcg cggcggcggt gttcggggtc
     3661 gcttggtatt tcagcgccga ggtgggcgcc aataaggaca aacgcgacga tctcatgcgg
     3721 gactactatt cctaccccat cgcacaacgg cccgaggtct accaaaaggc cctcgattat
     3781 gaggacaagg cgaaattcaa cgacacgatg cggatcgtga tgttctcggt gtcgggcgcc
     3841 gcggcggtcg ccggcacggt gctcgccatc tggcaatccg actggtcaca gagcgagccg
     3901 gcgccggatg cgccgaaggt atcgttctat ttcgacggca gttcggtcta cgccggcgct
     3961 tcgttcagtt actgagggag ggaacgatga ataccacgat gacacggatg accattatcg
     4021 cggcgctcgc cgtttttatc gcctcgtgcg agttcgtcac gaacaacgac ctgctcgaac
     4081 aactcaagaa ggaccctccg accggcacgg ccgacaccga cagcacggcc gataccgaca
     4141 cgacgggcac cgacgatctg ttgcccgaca gcgacgaact gacccccgat atagacacgg
     4201 tgctgacctg cggcaacaac cagttggaca ccggagagga gtgcgacggc aacgacgcgc
     4261 actgcgtaga ctcaccctcc ggtcgtacct gtaacaatga cacctgcgag tgcgtcgccg
     4321 tttgcggcaa cagcatcatc gaaggcgacg aagaatgtga ggccgaggac aacatcgaac
     4381 tctgtactga gatagatccc gcgaactatt ctggtggtga ggcggagtgc gatcacgaga
     4441 cctgcaagtg gattgtatcg gaatgtgtcg gcatgagtct ctgcgggaaa gagaacgccg
     4501 tctgcggacc gggtgatgat caggactgca cggacataga ccccgacacc tatgttggcg
     4561 gcacggcgac ctgcaacgac gactgcgatg gttggatgac taccgactgc tccccgaagt
     4621 gtacctgcgg caacggcgta ccggaaagtt tcatcaacgg gtgcgccacc accgaggaat
     4681 gcgatgacgg caactttgcc gatgatgacg gctgttcgtc cgattgccat tgggaggcca
     4741 tggtggcggt gaccgacctt gcggccgagc gcacgaccac cgagcccgcg gccgatatta
     4801 tctccttctc gtggacctgc gtcgggcccg gcgtgaccgg ctatatcctg aaaaagagcc
     4861 gctcccccat cgcgaatgac gccgattttg atgacgcaga ggtcctgcac aacgacacga
     4921 tctcctgcac cccggggacg gtccgcacca tagataccga gatggactgg tacgacggta
     4981 cctggcatgt cgcgatacgc cccgtgcgta acaacaccac cgggcccctt tccaactcag
     5041 tgacggtcga taccgacggt tacatggcgc tcgctcccga ggtcggcatc cagttcggac
     5101 tggtcggcta caacgcgtat ctggaccgtg cggtgacgct gaacaacacc tcggcgctgg
     5161 tggatctgat catagacgag accaccctgc cgggtgattg ttccggtaag gtgaccatcc
     5221 tttccggcga ggtctcttcc gccgcgaggg tgacggtcgt cccgggcaac ttccacgcgg
     5281 tggtactgcg ttatgcaccg accgaaacat catcctgcac agacggacag ttcgtggcca
     5341 aatggctttc ccattccgat gacggcggtg ccaccttcac cccggggcag gcctctctgc
     5401 cggtcagcgg caattcgggc aacactccgc cggtattcac cgatgtctac ttcaacccca
     5461 accctgtgaa aacgagcgag ggaacgaccg ctatcatggt ccagttcgag gatgacaacg
     5521 gcctctgcga cgacggcacc atcggcaacg acatcgtcga ggcggtgtgg gacatgcgtg
     5581 aactgggcgg cggcccgcgc gaggtcatga ccaatccctc ttgctcgacc gacacccacc
     5641 agtactggta ttggaagaac atcgatgtct cgtctctcac caacggcatc tatgtgatac
     5701 cgctcacact caccgacaag ggagacaaca ccgttcgcag gaatgtatcc ttcgccgtct
     5761 attcgggcac catcctcgag gtgggcaccg acaagaccta tacctcgatc gcccagccgg
     5821 tgatcgatgc acaggccggc gacatcgtgg tcgtgcatcc cggcacctac gactccatgg
     5881 aggtcgccaa cgaagacatc aaccccggcg gcgacgccat catcgtctac gggatagagg
     5941 gacccggtca aacggtcatc gatctgaccg acgcctccca ctccgggttc gaacttgaca
     6001 gcgccaacag cgggttcgtc atcggcggct tcacggtacg caacgccctg accggcgcga
     6061 tatgggccag cgccaccgcg acgaccatca aggtgacggt gaccgactgc cggttcgaga
     6121 ataatatcaa aaataccgac ggcggcgcca tcttcatttc tggcaacctt gtcgatctca
     6181 cggtggcaca ctctttgttc atcgggaaca gcgctccgag ccataccggc ggggccatct
     6241 acgcgtccga tgtccagaac ctgctggtgg tcgacaccct gttcgacggc aacaccgcgc
     6301 agaatggcgg cgcggtactc gttgagaaac cggcgcaagc agtgttcgac cgctgtttct
     6361 tcaccgacaa cacctcttcc acccaaggag gcgctattct tatcgacggt ggcgcgggga
     6421 tcgtcgagct ccgcaattcc atctttgccc ataacaccag cacctaccgc ggcgcagctt
     6481 tttatatcac caataccgtg agcaccatac cgacggtcaa gatatacaac tccaccatcg
     6541 ccgacaacac cgccggcacc gatgcgggcg gcctctatat cgaacggggc gatgtgaccg
     6601 taaaggacac catcatcttc gccaataccg ccgggaccga tctggccagt caactctatg
     6661 ccgtcaatga gaacggggtc accgtcacgg tcgacaacag cgtgatcggc ggcgcccctg
     6721 tcccggtcat cgatcccggc aacaagataa acgcccccga cggtctctgg accaaccatc
     6781 agaacctctc ggacgatccg ctgttcaccg cgaccgggaa tggcgcggtc cgctatcgcc
     6841 tccagcccgg gaccccctgt ctcgatgtcg gatcaaacgg ctcgaccgta ccgcagtacg
     6901 atttcctcgg ctacgcacga caaggggcga agaacgagat cggcgcgttc gagtaccttc
     6961 cctgaccatc tcccctatct ccatgaccgg caccgccgaa ctcttcggtc tcaccagagc
     7021 gcaacttgag gagacgatga ccagcctcgg catgaaacca ttctgcggcc gtcagatatt
     7081 ccggtggatc tatcagggac tcgagaccga cctgctcaag atgaccgaca tcgccaagga
     7141 ggcacggaac tcgct
//
LOCUS       DAOQUE010000087         7150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_11543_length_7150_cov_5.255250, whole genome shotgun sequence.
ACCESSION   DAOQUE010000087 DAOQUE010000000
VERSION     DAOQUE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11543_length_7150_cov_5.255250"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKH10_03955"
     CDS             complement(<1..243)
                     /locus_tag="PKH10_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27314.1"
                     /translation="MRKILVWLCAPVALFLVACESNEPGGIRLHYSWVDSEGNDLTPP
                     DLTILHAWGKLKYGSETKTSEAVWMGDAAATLRFDNL"
     gene            complement(347..1744)
                     /locus_tag="PKH10_03960"
     CDS             complement(347..1744)
                     /locus_tag="PKH10_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27315.1"
                     /translation="MKNIICALLVVALFNGQLFAAGAAPTKAEQKSGSVESSAVTSPP
                     PAAPDSENSSPGQAGEPKKLLVQRIAGVEVDEAITRTVEETIVLNIGNRAGYSVVTAA
                     EMESAIQFTGQQAEMQCESSTECLVEIQKKLDCDTLIAGKVAKLGDELILSLNTINVN
                     DGSVGLRTTAQAKDIADLKMKLPQALDEILGIAQMKQLFQLKPGETLKLAVMPLAARG
                     VEQATSDALTAILSSELNSIQGVNVIGQDDINAMLAKVQTDAEMQCTDSLECVVEIGA
                     SLGLSKLITGAVGKVKDTWVISIQLIDTRRGEVENRVLESFDGEKDELRNAIKLAAYQ
                     IAGVDYTAKKGGVELTFNVKEAALQLGAEKGDVKESQYKQAELVPGRYSLRVIADPED
                     YLPLQTDIYIAPGANNVKNMTVPEQPTPWYKSWWFWTVVGVGVAGAAAATTTVLLLEK
                     KDDGFVNMPLNPEGR"
     gene            2188..3450
                     /locus_tag="PKH10_03965"
     CDS             2188..3450
                     /locus_tag="PKH10_03965"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HNT27316.1"
                     /translation="MGEFSESLLSVEASSYTELPLSDDLAWLKQRIGKFVKGGIYLIA
                     GQPGIGKSTLGIQMCLDLGRSGEKSLFILTEQSKEDLRKRATLMCSKWPAGKVKTALG
                     NIAPEENIYDIENLPVFLAHQVLNPSGKYAGVKFIVVDSIQGQGLASAATRKYKQIYE
                     FCRQCKASGITVLLVCHVTKKGDISGPKDLEHNVDCVIVMRKAMVYRPLFVPKNRFGP
                     AILKPVPVQMDKQTTELKLSKHTEMVSSVAKTYLGRAVGVSEAQAAVTLPSYGTHGKI
                     TAPGLPRREIEQLTTCISQIPEMEIEDLDYTIHCRLPGEKRYRALLGLPVCMALIASY
                     IHKDIPPYYLYLGEIDLLRKIRSVPIELIVDLLEAIKEGLVQKPIRLFCPKETADLIV
                     EEVEGVTIISCESLDDAVFKTWPEIKEG"
     gene            3474..4568
                     /locus_tag="PKH10_03970"
     CDS             3474..4568
                     /locus_tag="PKH10_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27317.1"
                     /translation="MSIIDHLREITRTSRDVLSQLNQKLQQIQQNRGGIEERTKRLRS
                     EFSQFNRQENEARGGQKKLQETIDRLNAEMDELEKKRKQLITDISNAREEFDSTKNAI
                     TEIERKRQEYHRSITKLERELEKVAEEEIAVDEKRARHQQELNSARATAIPLYLDDLN
                     GKLFRMVEQSHNRKCSKEQFERFKKARHENEDVAKLCESRDEWQRILKSIMVPAVKES
                     VERELATIESEIKKQFPGALEFSAGSGGEEFIADLYYYNDSKDRCVILLPIKGSECPD
                     EETNKDKARSSFVISLFLQLCQQLAVKPETGRFFVDGNDGYYRLVLADRKNPLPDLKE
                     NYLIIELPDGEVATLVMATLPSEIMEAING"
     gene            4561..5046
                     /locus_tag="PKH10_03975"
     CDS             4561..5046
                     /locus_tag="PKH10_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27318.1"
                     /translation="MGKALTSLSKELKIDLPNLVLHLASLGAPLEGIWPEVDKAWLAY
                     LVNIDWGKFGEKESKGKQEVDKEKEVKDEGALERAKIVFKLDRKSFYGARTVSKETMK
                     NHFFQDMPNFEKRMEELLELAYLLKDKEGKYALNMSKKKNIENMVAVYVEEKEAGSKG
                     P"
     gene            5262..5624
                     /locus_tag="PKH10_03980"
     CDS             5262..5624
                     /locus_tag="PKH10_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27319.1"
                     /translation="MFSYTLALVLVLDIAYIFCEKGLAQLLKTQSSPQQSVDSQLGIK
                     NDHGFVKTVSPTIQINGRNNTVILVDENSPGAIEMLERAYNNMKEEQDIDKALKAIQS
                     NDSVFDDDDFVVDQEGRK"
     gene            5621..6535
                     /locus_tag="PKH10_03985"
     CDS             5621..6535
                     /locus_tag="PKH10_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27320.1"
                     /translation="MSRLIFAAALIFFFGIPVILSADNNKNDNDRCLVSKTKSIGDLE
                     NIRSYCDELSKIRANITLGEKYSEEDNLERNKKAISYFLKAIDIIEKSSQKDEYKTWE
                     FISFKYMSYVYSNKKFSLDKFLEITKKAYGLSQEIGINDNPITYNMVVANYRKALALK
                     YMGKTEGDYGKYLRESILYSGLLVKSESNMHKTYTLDILARIANEFDQDCDKATEYYN
                     TISRDLLSLPQYQYVITNMINGYMSCAEKANKTLAKDNNRDEMLEKETCKKIKEWYMA
                     ALNISNDKKLKGKIADRLKNVCKKCSCK"
     gene            6567..6833
                     /locus_tag="PKH10_03990"
     CDS             6567..6833
                     /locus_tag="PKH10_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27321.1"
                     /translation="MGNKRTYDAIPPKYGKSKTAKRIISKQETGEDCYTTVLYFENGN
                     MKIETFVTESIEKGVELAKKYIYSEFDKDYHFKETTGSATCPFC"
     gene            complement(6968..>7150)
                     /locus_tag="PKH10_03995"
     CDS             complement(6968..>7150)
                     /locus_tag="PKH10_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27322.1"
                     /translation="CLLAYSFGGSITVTEATNTSAASGGSLAFVGADLLLYYPTETPV
                     GDISSKLSGITVCPKE"
BASE COUNT         2009 a         1533 c         1868 g         1740 t
ORIGIN      
        1 gaggttgtcg aaccggagcg tcgcggcggc gtcgcccatc cagaccgcct cggaggtctt
       61 ggtctcggag ccgtatttga gtttgcccca tgcgtggagg atcgtcaggt cgggcggcgt
      121 gaggtcgttc ccctcgctgt ccacccatga gtagtggagc cgtatgccgc ccggctcgtt
      181 gctctcgcag gcgacgagga acagcgcgac cggcgcacac agccagacga ggatctttct
      241 catgatctca cctcctcatc gggcgcgatg aactgcgccc ctacagtatg tgccctcgcc
      301 ctgttcaggg agagggatgg gtgagggtgt tcttgcacgc agagcctcac cgcccctccg
      361 ggttcagcgg catgttgacg aacccgtcgt ccttcttctc aagcaggagg acggtggtgg
      421 tcgcggcggc ggcgccggcg accccgaccc cgacgacggt ccagaaccac cacgatttat
      481 accacggggt gggctgttcg ggcaccgtca tgttcttcac attgttggcg cccggcgcga
      541 tgtagatatc ggtctggagc ggcagataat cctccggatc ggcgatgacc cgcagactgt
      601 agcgaccggg gaccagttcg gcctgcttgt actggctctc cttgacatcg cccttttcgg
      661 cgcccagttg cagtgccgcc tccttgacat tgaaggtcag ttccacgccg cctttcttcg
      721 cggtgtaatc gacgcccgcg atctggtagg cggccagttt gatggcgttg cgcagttcgt
      781 ccttctcgcc gtcgaaactt tcgagcacgc ggttctcgac ctcgccgcgc cgcgtgtcta
      841 tcagttgtat cgagatgacc caggtgtcct ttaccttgcc gacggcgccg gtgatgagtt
      901 tcgagaggcc gagcgacgcg ccgatctcga cgacgcactc gagactgtcg gtgcactgca
      961 tctcggcgtc ggtctgcacc ttcgcgagca tcgcgttgat gtcgtcctgc ccgatgacat
     1021 tgaccccttg aatggagttg agttccgacg aaagtatcgc ggtgagcgcg tcggatgtcg
     1081 cctgttcgac gccgcgcgcc gcgagcggca tgacggcgag tttcagcgtt tcccccggct
     1141 tcaactggaa caactgtttc atctgggcga tacccagtat ctcgtcgagc gcctgcggca
     1201 gtttcatctt cagatcggcg atatccttcg cctgcgccgt ggtccgcagt cccaccgagc
     1261 cgtcgttcac attgatggtg ttgagcgaca ggatcagttc gtcgcccagt ttggccacct
     1321 tgcccgcgat gagggtgtcg cagtcgagtt tcttctgtat ctcgacgagg cattcggtgg
     1381 acgattcgca ctgcatctcg gcctgctggc cggtgaactg gatggcgctc tccatctcgg
     1441 cggcggtgac caccgagtag ccggcgcggt tgccgatgtt gaggacgatg gtctcctcga
     1501 ccgtccgggt gatcgcctca tcgacctcga ccccggcgat acgctggacc agcagtttct
     1561 tgggttcgcc ggcttgtccc ggcgaagaat tctcggagtc gggagcggcg ggcggcgggc
     1621 ttgtcacggc ggagctttca acggagccgg atttctgttc cgcttttgtc ggcgcggcgc
     1681 cggcggcgaa gagttggccg ttgaacagcg caacgaccaa aagcgcgcag atgatgttct
     1741 tcatggaaat ctccctcatc tatgataata ttctcccacg ctccttcgat gcagacccga
     1801 aatcataacc ggttcgtttt aaatgtaaaa aaataaattc taaggtaggg tgaaaaaaaa
     1861 gaatcctttt ggatcggcgg ggtatcttat ggggtgactg aggaaatgcc tcaaccggct
     1921 ttatcaaacc tcaccccggg tcctccgccg taggctacgg cccgcaggcg gaccctctcc
     1981 atcgtggaga gggtgacaca gtccttctcc gtttcggaga aggtgaagga tgaggttgaa
     2041 gtggagaagg tgaagaggaa ggcaaagaaa aatgtatgaa tttcttgatt gcggattttt
     2101 gtgggcgtat tatagcggag tcagcaggga ggttgggcgt ctgaataagt ttaaagggga
     2161 agaatttctt tcaaacagga ggatttcatg ggtgagttca gcgaaagcct gttatcggtt
     2221 gaggcatctt cttataccga attgccttta tcagacgacc ttgcttggct taagcaaagg
     2281 atcggtaagt ttgtaaaagg cggcatatac ttaatagccg ggcagccggg tataggtaaa
     2341 tccacgcttg gaatacagat gtgccttgat ttggggcggt caggcgaaaa aagccttttc
     2401 atccttacag aacagtcaaa ggaagattta aggaaaagag ccactcttat gtgctcgaaa
     2461 tggcctgccg ggaaagtgaa gacggcactt ggcaatatcg ctccggagga aaacatttac
     2521 gacatagaaa atctaccggt gtttcttgcg catcaagttc tgaatccatc gggcaaatat
     2581 gctggtgtta agtttatagt ggttgattcc atacagggac aagggcttgc gtctgcagcg
     2641 acaagaaagt acaaacagat ttatgaattc tgtcgtcaat gcaaggccag cggaatcacc
     2701 gtgcttcttg tttgccatgt aaccaagaag ggagacattt cgggccccaa ggacctagag
     2761 cataatgtcg attgtgtgat tgtaatgcgt aaggccatgg tttatcgtcc gcttttcgtg
     2821 cccaaaaatc gcttcggacc agcaatcttg aagcctgttc cggtacaaat ggacaagcag
     2881 acaacggagc tgaagctatc caaacatacc gaaatggtta gtagcgtggc aaagacatac
     2941 cttggtcgcg ctgtcggggt gtcagaagcg caggcggccg ttactctgcc ttcgtatgga
     3001 acacatggaa aaattacggc tcccggactg cctcgcagag aaatcgaaca gcttacgact
     3061 tgcataagtc agattcccga gatggagata gaagatcttg actacacgat ccattgtcgt
     3121 ttgccgggag aaaaaagata tcgcgctttg cttggccttc ccgtttgtat ggcgctcatc
     3181 gcatcgtata tccacaagga catcccgcca tattaccttt atctcgggga aatagacctt
     3241 cttagaaaaa tccgctctgt gccaattgag ttaatcgttg accttcttga agctataaaa
     3301 gaagggttgg tgcaaaagcc catcagactg ttttgtccca aagaaacggc cgatcttatt
     3361 gtggaggaag tcgaaggcgt tacaattatt agttgcgaaa gccttgatga tgcagttttc
     3421 aagacttggc ccgagattaa agaaggctga ttgagagtgg cgggggggag aaaatgagta
     3481 taattgacca cctaagggaa atcacgagaa cttcccgcga tgtgttgtcg caactaaacc
     3541 agaaattgca gcagattcag caaaacagag gcggaattga ggagcggaca aaaaggttgc
     3601 gctctgaatt ctcccaattc aatcgtcaag agaatgaagc gaggggaggg cagaaaaaac
     3661 ttcaagagac tatagatagg cttaatgctg aaatggatga gttggaaaag aaaagaaagc
     3721 aactcatcac agatatctct aatgcaagag aagaatttga ttcaacaaag aatgcaatta
     3781 ccgaaatcga aaggaagcgc caagaatacc acagaagcat tacaaaactt gaacgtgaac
     3841 ttgaaaaagt cgccgaagaa gaaattgctg tagatgaaaa gcgcgcgaga catcagcagg
     3901 aattgaattc tgctcgggcg acagccatcc cattgtatct tgatgatctt aacggaaaac
     3961 tgttccggat ggttgagcaa tcacacaaca gaaaatgcag caaggagcag tttgagcgat
     4021 tcaaaaaagc ccgacatgag aacgaggatg tggccaaact ctgtgaatca agagatgagt
     4081 ggcaacggat actgaaaagc attatggttc cagcggtaaa agaaagtgtc gagcgtgaat
     4141 tggcgaccat tgaaagtgaa atcaaaaagc aatttccggg tgcgcttgag ttctcggcgg
     4201 gatcgggcgg cgaggaattt atcgcggatc tttattacta caatgacagc aaagatcgtt
     4261 gcgtgatttt gttgccgatc aaaggttctg aatgcccgga tgaagaaacc aataaagata
     4321 aggcgcgttc ctcttttgtg atttctttat ttctgcaact gtgccagcag ttagcggtaa
     4381 agcccgaaac cggtcgattc ttcgttgacg gcaatgatgg atattatagg ttggttcttg
     4441 cggacagaaa aaatcctttg cctgatttaa aagagaacta tttgatcata gagcttcccg
     4501 atggtgaggt tgccactctc gttatggcta cacttccaag tgaaataatg gaggccatca
     4561 atgggtaagg cactgacctc tctttccaaa gaactaaaga ttgatttgcc taaccttgtg
     4621 ttgcacctcg catcacttgg ggctccgtta gaaggtattt ggccagaagt tgataaggca
     4681 tggctggcgt atcttgtaaa tattgactgg ggaaagttcg gagaaaaaga gtcgaagggc
     4741 aaacaagagg ttgataaaga gaaagaggtg aaagacgaag gtgcccttga acgggcaaaa
     4801 atcgtgttca agcttgacag aaaatctttt tatggtgcgc ggactgtatc taaagaaaca
     4861 atgaagaatc atttttttca agacatgccg aattttgaga aaagaatgga agaattattg
     4921 gaattggcgt acttgctcaa agacaaagag ggcaaatatg ctttgaatat gtctaagaaa
     4981 aagaacatag aaaatatggt tgcagtttat gtcgaagaaa aagaagcagg aagtaagggg
     5041 ccgtgagttg taagatacca tatccagaag taatttccgc gaggtttttt aaacatggtt
     5101 cctcttaatc aacgagttta aagatgataa taaaaacagc cctcagtatg attcttacct
     5161 ttataataac taaaggtgtc cgggggttaa taaagtatcc caaaagaaat gtatgtaact
     5221 tctgttgctt atcaaaaaag agttcttcat ggtatacaaa gatgttttct tataccttgg
     5281 cgttagtttt ggtccttgat attgcctata ttttttgtga aaagggattg gcgcaattgt
     5341 tgaagacgca atcttcgccg caacaaagcg tggatagcca attgggaata aaaaatgacc
     5401 acgggtttgt aaaaaccgtc agtccgacaa ttcaaataaa cggacgaaac aatactgtta
     5461 ttcttgtaga tgagaacagc ccgggagcaa tagaaatgct ggagcgtgcg tataataata
     5521 tgaaagaaga gcaagatatc gacaaggcat taaaagccat tcagtctaat gattctgttt
     5581 ttgatgacga tgactttgtt gttgatcaag agggtagaaa atgagccgac tcatatttgc
     5641 cgcagcttta atttttttct tcggcatccc cgtcatatta tctgcagata ataataaaaa
     5701 tgataacgac agatgcttgg tttcaaaaac aaagagcatc ggagatttgg aaaacataag
     5761 aagctattgc gatgaacttt ctaaaattag ggcaaacatc acattgggcg aaaaatattc
     5821 tgaagaggac aatcttgaaa gaaacaagaa ggcaatatca tattttctaa aagctataga
     5881 tattatcgaa aaatcttccc aaaaagatga atataagaca tgggagttta tatcctttaa
     5941 atatatgtct tatgtatatt caaataagaa gttctctttg gataagtttc tagaaatcac
     6001 gaaaaaggca tatgggttaa gtcaggaaat aggtatcaat gataacccga taacttataa
     6061 catggtggtt gcaaattata ggaaagcatt ggcccttaaa tatatgggaa aaacagaggg
     6121 tgattatggc aagtatctta gagaatcaat attatactca ggtctgttgg taaaaagcga
     6181 atcaaatatg cacaaaacat acactcttga tattctggca aggatagcga atgaatttga
     6241 tcaagattgt gataaggcca cggaatatta taatactatc tctcgcgatc ttttgtcatt
     6301 gccacaatat cagtatgtta taacaaacat gattaacggt tatatgtcgt gtgcggagaa
     6361 agccaacaag accttggcca aagataataa tcgcgatgaa atgttagaaa aggaaacatg
     6421 caagaaaatc aaagaatggt atatggcagc attgaatatt agcaacgaca agaaattgaa
     6481 agggaagata gcagataggt taaagaatgt ttgcaagaag tgttcttgca agtaatcaaa
     6541 atcaactatt agccggaggt cgcccgatgg gaaacaaaag aacttatgat gcaatccccc
     6601 cgaagtatgg gaaatctaaa actgctaaaa ggataatatc aaaacaggaa acaggggagg
     6661 actgttacac aacggttctt tatttcgaaa atggcaatat gaagattgag acatttgtaa
     6721 ctgaaagtat tgaaaaaggc gttgagcttg ccaagaagta catatatagt gagtttgata
     6781 aggattatca ttttaaagag actactggca gtgcgacttg tcctttttgt tagttctttt
     6841 tggggatcca acggtatcag atctcaagaa gtgaataact gaccaatcgc gccgccccct
     6901 ctcttggcat ttaatccccc tcccagcacc cggcaaggag gcgcgcgatg aaggaatagg
     6961 gcaatcgtca ctccttcggg cagaccgtga tcccgctcaa tttactggag atatcgccca
     7021 ccggcgtctc ggtcggatag tacaatagaa gatcggcgcc gacgaaggcg agcgagccgc
     7081 cgctggccgc cgaggtgttc gtcgcctcgg tcaccgtgat cgagccgccg aacgaatagg
     7141 cgagcagaca
//
LOCUS       DAOQUE010000088         7028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_11815_length_7028_cov_3.941775, whole genome shotgun sequence.
ACCESSION   DAOQUE010000088 DAOQUE010000000
VERSION     DAOQUE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11815_length_7028_cov_3.941775"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PKH10_04000"
     CDS             complement(<1..1156)
                     /locus_tag="PKH10_04000"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNT27323.1"
                     /translation="MFILLYALLSAEENPYRTVVSEQAEETPATGATTVITEKEIEKK
                     QKGQVADLLRETPGIHVATSGGAGGITSLFIRGGKAGHALVLLDGVRLNDPLSVENAA
                     DLSDITADTVSRIEILRGPQAVIYGPDAMSGVVRIVTKRGSGHPALSLTGESGMVDLS
                     TDHIAVNHLRTGASLRGGTEDGDYAAAIYYRDTGGYSAAAERDGNIEEDRSRLLTLNG
                     RAHLFLSDHLTLGASVRAASADTDLDNGPGRYRDDINHTFASDRLLGRVSLDGSFFDG
                     RWLPSWSVAVSHHACDDHNAPDGTGDEWGFNHAGFRGETVEAAWRNDITALPLQRLRA
                     GLVFNAGFGGSDHRTDRERDITALDIERKDTYGLGIYLQDTLTPVEGLDLI"
     regulatory      complement(1197..1375)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1456..2874
                     /locus_tag="PKH10_04005"
     CDS             1456..2874
                     /locus_tag="PKH10_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HNT27324.1"
                     /translation="MERYVVKTSLLAVALLLFACAETKNTDTDHDTIVDGDDAVTDAD
                     IDTARVFTQRRILGEGGFFGISSSAGNDDYFIIGAHYAELSATPGENPSGKVYLYKKG
                     TIPTLFSDAETVLTHPDERPGAGFGYTIGGYCDLNCDGAADFAVSAHLDSVGDTPNCG
                     TIALFFGGDADPATAVLSLPDGVRRQSDVMGQSLVCFDIDGDGCDDLVAGGQNAGPND
                     TGLIAVWKGAAAGPAAEPAFYMEAQLAENKQYLGASLAVGDVDGDGTPDLVAGGWGLK
                     KDAAAANTGGVYLYKTGDDWSQGPSAVLFPDETVAQEVGFGSKVILFTVDGVTYIAVM
                     APDYNQEAGAVFIYYFASGAFRSHAMFKPSTYGDPVYGISDIAFSPDFGGKGKGALLI
                     GHKYYGADSTGAVTAVSFPEWTPVVITAEPPVSGDTFGAMVKDIGDINGDGLADFVVG
                     TPEHIEGDFETGTQPGGIVLLY"
     gene            2886..3587
                     /locus_tag="PKH10_04010"
     CDS             2886..3587
                     /locus_tag="PKH10_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27325.1"
                     /translation="MTKQKPVLCLLLFLVVSVCFSFSCSHDNENPSASSKQRKIPDST
                     ATPQTDTQEQDKNIPKTLDEAHALLEKQLPKEELAKIDAMKSEEEMNGFHFGLGMGLR
                     NGWDLWNGSPLSKHMNDLGFFHPDDMSGVILDTFWCKRHKKDFRLKERAEYYKTWWLA
                     VAKPPSTSMDPQDQSEIDWLMSLNIGNEKRPQIIHAGKSKKSKRWLVYEYDKGVYVPN
                     KELLEKIKKQEEYSR"
     gene            complement(3735..4595)
                     /locus_tag="PKH10_04015"
     CDS             complement(3735..4595)
                     /locus_tag="PKH10_04015"
                     /inference="COORDINATES: protein motif:HMM:NF023701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HNT27326.1"
                     /translation="MREKLINEEALLTVLRHLKRKDILSEVVIIGGSVLYLYRAHFAD
                     SDIPPYATKDLDLLLPEARNDLPQIDLPAILAECGFIGDASYTYTHPDIDIDILTPRH
                     ERCRGSRTFLKGYNTFALAEVHLDLLRDHTQSIRYGGLLVTVPRIEAFTCHRFLIAPE
                     RVDEPDRDKDHAVAVALADIVLSKKTLCDAFCHIAESRDRSSREALYRTLFRHKPALY
                     GVLSLFCLGRFIDAEKVEKGPERKALQVAEKVIRLEGLLAYHNPIYREVRNGLEPKMI
                     AAFAQEHKNR"
     gene            complement(4576..4932)
                     /locus_tag="PKH10_04020"
     CDS             complement(4576..4932)
                     /locus_tag="PKH10_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27327.1"
                     /translation="MSVIQHLLGDERSRLLRLIALWSEKLADPKYPQGRLYVKKAKGR
                     EYLYHCYYDRGRRMQKFISPRYDERSLDLQLLFRERTAVEKELRAMEENLAEIEVALN
                     AVNQKRRKDADAGKTH"
     gene            5101..6711
                     /locus_tag="PKH10_04025"
     CDS             5101..6711
                     /locus_tag="PKH10_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNT27328.1"
                     /translation="MELISQHVKGIMEECKRRARAAGLSFDNESLEYIVTNRDMIELS
                     PKVMIPTLYDYWVSDVEVLKEQGKYKLYPFNPYETVINSRPAISFYNDNNPDWLNVMI
                     FYHVLGHIDMFQNNILFKNTWTDDFVGQALADKRLIANLRSEYGRWVDYVIEFARGID
                     NITGYFSEMAEREYPAELDPPERLAFYFGPFLQEIVKLPTADYYRELERYNQLVDEKG
                     AAAEEMFFGEVRGRYPEFPAKYERHGMKRPARYRDLLQYVHDNSPFLKKEQNGWMRSI
                     LQIVRNTALYFQPQIRSKILNEGWASYWHEELFLRDERIIGHESDYARVNSGVTSISR
                     VGLNPYAVGLRLIRWVEELADKGKMSRRFQQIHDANLREAFNEKTGAGKKAIFALRDN
                     FSDFMLINTFVDQEFVDRFDLFVVGKRLDEARGVWQYYIKSRKADDYKQMLLEHLYHP
                     PVITVDEGRTTEKSLALVHQFEGKQLVKDFIGDTLIGIEFLWGGEVTLDTTEITVKKP
                     ETEGAEPQREYRRVRYHCSERKIVRETL"
     gene            6737..>7028
                     /locus_tag="PKH10_04030"
     CDS             6737..>7028
                     /locus_tag="PKH10_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNT27329.1"
                     /translation="MRTHTCGELRLTDAGKSATLSGWVVSKREFGPFIFIMLWDRYGQ
                     TQVVFEEPLLVEEAKKLSLEDVVRVEGFVRDRGDNRTDKYETGAIEVKAATIE"
BASE COUNT         1515 a         1988 c         2103 g         1422 t
ORIGIN      
        1 cgatgaggtc gagcccttcg accggcgtaa gggtgtcctg caggtatatc ccgagtccgt
       61 aggtgtcctt gcgctcgatg tcgagcgccg tgatgtcgcg ttcgcggtcg gtccggtggt
      121 cgcttccgcc gaaccccgcg ttgaagacga gcccggcacg gagacgctgg agcgggagcg
      181 cggtgatgtc gttgcgccac gccgcctcga ccgtttcgcc gcggaagcca gcgtgattga
      241 aaccccactc atcacccgtg ccgtcgggcg cgttatgatc gtcgcaggca tggtgggaga
      301 ccgcgacgct ccacgacggg agccaccgtc cgtcgaagaa cgacccgtcg agggagaccc
      361 gtccgagcag acggtcgctc gcgaaggtat ggttgatgtc gtcacggtat cgccccggac
      421 cattgtcgag gtcggtgtcg gcgctcgcgg cgcggaccga tgcgccgagc gtgaggtggt
      481 cggagaggaa cagatgggcg cggccgttca gggtaagaag gcgcgaacgg tcctcctcga
      541 tgttgccgtc gcgctcggcg gcggcggagt agccgccggt gtcgcggtag tatatcgcgg
      601 cggcgtagtc gccatcctcg gtgccgcccc ggagcgacgc gccggtccgc aggtggttaa
      661 cggcgatgtg atcggtcgaa aggtccacca tgccgctttc gccggtgagc gaaagggccg
      721 gatggccgct cccgcgtttt gtgacgatgc ggaccacccc gctcatcgcg tcgggaccgt
      781 agatgaccgc ctgcggcccg cgcagaatct cgatgcgtga caccgtgtcg gcggtgatat
      841 cggaaaggtc ggcggcgttc tcgaccgaca gcgggtcgtt gagtcgcacc ccgtcaagca
      901 ggacgagggc gtgccccgcc ttgccgccgc ggatgaagag cgaggtgatg ccgcccgcgc
      961 cgccggaggt cgccacatgg atgcccggcg tctcgcgcag gaggtcggcg acctgacctt
     1021 tctgcttctt ttctatttcc ttttcggtga tgacggtggt cgcgccggtc gcgggcgtct
     1081 cttcagcctg ttccgatacc acggtgcggt agggattctc ctcagcggaa agaagggcgt
     1141 acaacaagat gaacatgagg agaacgcgca ccggcctcat ccctccgcgg ggcagttacc
     1201 gtccgatatc ctccgcccgg gtattctggc ttgcggtcat cctacttgcc gccccttccc
     1261 gccggtctcc cggcagtggg cttctgcggc gttcgtcgcg cttacagctg cgggacagcg
     1321 ccggtcttgc accggcttcc ccgcgcggag aacgaaccga tacccctgta tcatacgggg
     1381 cgaccggtgt caagcgccgt cttgctttta tcgggcgcgg cgggtatgct tgagcggtcg
     1441 tcagggaggt atgcgatgga acggtatgtg gtcaaaacaa gtttgcttgc ggtggcgctc
     1501 ctcctgttcg cctgtgccga gaccaagaac accgacaccg accatgatac tatcgtcgat
     1561 ggcgacgatg cggtgaccga tgccgacatt gacacggcgc gggtcttcac ccagcggcgc
     1621 atcctcggcg aaggcgggtt cttcggcatc tcttcatcgg ccgggaacga cgactatttc
     1681 atcatcggcg cccactacgc cgaactttcc gcgacgcccg gcgaaaatcc gtcgggcaaa
     1741 gtgtatcttt ataaaaaagg aactattccg accctcttca gcgacgccga aacggtgttg
     1801 acccatcccg acgagcggcc cggcgccggg ttcggctaca ccatcggcgg ctactgcgat
     1861 ctcaactgcg acggcgcggc cgactttgcc gtctcggcgc acctcgactc ggttggcgat
     1921 acacccaact gcgggaccat tgcgctcttt ttcggcggcg acgccgatcc cgccacggcg
     1981 gtgctgtcgc ttcccgacgg ggtccgcagg cagtccgatg tgatggggca gtcgcttgtc
     2041 tgcttcgata tcgacggcga cggctgtgac gatctcgtgg cgggcggtca gaacgccggg
     2101 ccgaacgaca cgggcctcat cgcggtctgg aaaggggcgg cggcgggacc ggcggccgaa
     2161 ccggcgttct acatggaagc gcaactggcg gagaacaagc agtaccttgg cgcgtcgctc
     2221 gccgtcggtg atgttgacgg cgacgggacg cccgaccttg tggcgggcgg ctgggggctc
     2281 aagaaggatg cggcggcggc caataccggc ggtgtctacc tgtataaaac gggcgacgac
     2341 tggtcgcagg ggccgtcggc ggtgctcttc cccgatgaaa cggtggcgca ggaagtaggt
     2401 tttggaagca aggtcatcct tttcactgtt gatggagtga cttatattgc ggtgatggcg
     2461 cccgattata atcaagaggc tggcgcggtt ttcatctatt actttgcgtc tggcgctttt
     2521 agatcccatg cgatgttcaa accttcgacg tatggtgatc cggtatacgg catctccgat
     2581 attgcttttt cacccgactt tggcggaaaa gggaaggggg cgcttcttat cggacacaag
     2641 tactatggtg ccgattcgac cggcgcggtc accgcagtat cgttccccga gtggacgccg
     2701 gtggtcatca cggccgagcc gccggtatcg ggcgacacct tcggcgcgat ggtaaaggac
     2761 atcggcgata tcaacggtga cgggcttgca gacttcgtgg tcgggacacc ggaacatatc
     2821 gagggggact ttgagacggg gacgcagccg ggcggcatcg tccttttgta ctaggggggg
     2881 gatccatgac aaagcaaaag ccagttctgt gccttttgtt gtttctagta gtttctgttt
     2941 gctttagttt ttcttgttca catgacaacg aaaatccatc agcgtcgtca aagcaaagaa
     3001 aaatacccga ttcaacggca actcctcaaa ccgacacgca agaacaagat aaaaatattc
     3061 ctaagacttt ggacgaggcc catgctttgt tggaaaaaca acttcccaaa gaagaattgg
     3121 caaaaataga tgcaatgaag tctgaagaag aaatgaatgg tttccacttt ggtctcggta
     3181 tgggattgcg taatgggtgg gatctctgga acgggtcacc tttgtcgaag catatgaatg
     3241 acctcggttt tttccacccc gatgacatgt ctggagtgat tctggatact ttttggtgta
     3301 agagacataa aaaagacttt cgtcttaaag agcgcgccga atattataaa acctggtggt
     3361 tggcagtggc aaaacctccc tcaacctcga tggatccgca agatcaatcc gaaattgatt
     3421 ggttgatgtc tttaaacatt ggaaatgaga agaggccaca aataattcat gcggggaaaa
     3481 gcaagaaatc caaacgatgg cttgtctatg aatatgacaa aggagtctat gtgcccaaca
     3541 aagaattgtt agaaaagata aaaaaacaag aggagtattc tagataaagc atttaaaaca
     3601 aatgctcaac aagccgtcgc ggctgaaaat gtcagaaaac aaactgattg agagacacat
     3661 tttcgatttg aattgctttt gttttcggca atcgctatgc tgtacccggt gtaacgaaca
     3721 aggacgaggg tggactatcg gttcttgtgt tcttgggcga aagccgcgat catttttggc
     3781 tccagtccat tgcgcacctc tctatatatc gggttatgat acgcgagcaa tccttccaac
     3841 cggatgacct tttccgctac ctgtaacgcc ttccgttccg ggcctttctc gaccttttcc
     3901 gcatcgatga atctccccag acagaagagc gataagactc cgtataatgc cggtttgtgc
     3961 cggaaaagag tccggtacag cgcctcccgc gaagagcggt cgcgcgattc ggcgatgtgg
     4021 caaaatgcat cgcacagggt ctttttcgac agtacgatat cggcgagcgc gacggcgacg
     4081 gcatgatctt tatcgcggtc cggctcatca acccgttccg gcgctataag aaaacgatga
     4141 caggtaaagg cctcgatacg cggtaccgtg accagcagtc cgccataccg gatgctctgc
     4201 gtatggtcgc gcaaaagatc gagatgcact tcggccagcg caaaggtgtt gtaccctttc
     4261 aagaaagtgc gggaaccgcg acaccgctca tggcgcggcg tcagtatgtc tatatcgata
     4321 tcggggtgcg tgtaggtata ggatgcgtca ccgataaaac cgcattcggc aagtatcgcc
     4381 ggtagatcga tctgcggcag gtcgttccgg gcttcaggca acaggaggtc cagatccttc
     4441 gtcgcatacg ggggaatgtc gctgtcggca aaatgggcgc ggtacaggta aagcacgctc
     4501 ccaccgatga tcacgacctc cgataggata tccttgcgct ttaaatggcg gaggaccgtc
     4561 aggagggcct cttcgttaat gagtttttcc cgcatcggcg tccttcctcc gtttttggtt
     4621 caccgcattc agggccactt cgatctcggc gagattctct tccatcgccc gcaactcttt
     4681 ttctacggct gtccgttcac gaaagagcag ttgcaggtcg agtgaccgtt cgtcgtagcg
     4741 tgggctgatg aacttctgca tccggcggcc gcgatcatag tagcaatgat agagatattc
     4801 gcggcctttc gcttttttga catagagacg gccctgcggg tatttcgggt cggcaagttt
     4861 ttcggaccac agcgcgatca ggcggagcag acgcgaccgt tcgtcaccga gaagatgttg
     4921 aatgaccgac aaagcgacct ccgttatcac aaaaacaccg aatagagcat atacttgtga
     4981 taactatttg tcaagtgttt aatccggcga acaggccgtt attgcggaat tgagttgctt
     5041 ttatttttag cggttgctat gctgtacccg gtgtaacgaa caaggacgag ggccgttgcc
     5101 atggaactca tcagccagca tgtgaaaggc atcatggagg agtgcaaacg ccgcgcccgc
     5161 gccgccggcc tctccttcga caacgaatcg ctcgaataca tcgtcaccaa ccgcgacatg
     5221 atcgaactgt cgccgaaggt catgataccg acgctctacg actactgggt gagcgatgtc
     5281 gaagtgctca aggagcaggg gaaatacaaa ctctacccct tcaaccccta cgagacggtc
     5341 atcaactcgc gtcccgcgat ctcgttctac aacgacaaca accccgactg gctcaatgtc
     5401 atgatcttct accatgtcct cggccacatc gatatgttcc agaacaacat acttttcaag
     5461 aacacctgga ccgacgactt tgtcgggcag gcgctcgccg acaaacggct catcgccaac
     5521 ctgcggagcg aatacggccg ctgggtcgac tatgtcatcg aattcgcgcg cggcatcgac
     5581 aacatcaccg gctacttctc cgagatggcg gaacgcgaat atcccgccga actcgatccg
     5641 cctgagcggc tcgccttcta cttcggcccg ttcctgcagg agatcgtgaa actgccgacc
     5701 gccgactact accgcgaact ggagcggtac aaccaactgg tcgacgaaaa gggggcggcg
     5761 gccgaggaga tgttcttcgg cgaggtgcgc ggccgctatc ccgaattccc ggcgaagtac
     5821 gaacggcacg ggatgaagcg cccggcgcgt taccgcgacc tattgcagta tgtccacgac
     5881 aactcgccgt tcctcaagaa ggagcagaac ggctggatgc gctcgatact ccagatcgtc
     5941 cgcaacacgg cgctctactt ccagccgcag atacggtcga agatactcaa cgaagggtgg
     6001 gcgagctact ggcacgagga actgttcctg cgcgacgaac ggatcatcgg ccacgaatcg
     6061 gactatgcgc gggtcaactc cggcgtgacg agcatcagcc gcgtcgggct caacccgtac
     6121 gccgtgggac tgcggctcat ccgctgggtc gaggaactgg ccgacaaggg caagatgtcg
     6181 cgccgtttcc agcagataca cgacgcgaac ctgcgcgagg cgttcaacga aaagaccggc
     6241 gccgggaaga aggcgatatt cgccctgcgc gacaacttca gcgacttcat gctcatcaac
     6301 accttcgtcg atcaggaatt cgtcgaccgg ttcgacctgt tcgtggtcgg caaacggctc
     6361 gacgaggcgc gcggcgtctg gcagtactac atcaagagtc gcaaggccga cgactacaaa
     6421 cagatgctcc tcgaacacct ctaccatccg ccggtcatca cggtggacga ggggcgcacc
     6481 accgaaaaga gtctcgccct cgtccatcag ttcgagggaa agcaactggt caaggacttc
     6541 atcggcgata ccctcatcgg catcgaattc ctctggggcg gcgaggtgac actcgacacc
     6601 accgagataa cggtgaagaa gcccgagacg gagggagcgg agccgcagcg cgaataccgc
     6661 cgcgtccgct accactgctc cgagcgcaag atagtgagag aaacactctg agaacgaacc
     6721 acaggaggtc cggtacatga gaacacatac ctgcggagaa ctccggctga ccgacgccgg
     6781 aaaaagcgcg acgctgtcgg gctgggtcgt ctcgaaacgc gagttcggcc cgttcatctt
     6841 catcatgctg tgggaccgct acggccagac gcaggtggtg ttcgaggagc cgttgctcgt
     6901 cgaagaggcg aagaaactct cgctcgagga tgtcgtaagg gtcgaagggt tcgtccgcga
     6961 ccgcggcgac aatcgcaccg acaagtacga gaccggcgcg atagaggtga aggcggcgac
     7021 catcgaac
//
LOCUS       DAOQUE010000089         6949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_11990_length_6949_cov_3.729765, whole genome shotgun sequence.
ACCESSION   DAOQUE010000089 DAOQUE010000000
VERSION     DAOQUE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11990_length_6949_cov_3.729765"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..4012)
                     /locus_tag="PKH10_04035"
     CDS             complement(284..4012)
                     /locus_tag="PKH10_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNT27330.1"
                     /translation="MNINIFRPLYEFLGFPYVGPYFTMLLFSFFVAFPLTAFLAPRFS
                     LRSRRLLMLTAVAIVASLAGSKLFHLLFDGQWSRYATIYREQGLVPFLAVAVNPVSAG
                     QVFYGGFIVAYLVSYLFIRRVWREPTGCYGDIAAFPVALGIALARIGCFFAGCCFGLP
                     SKAFGVSFPAYTPAAAELYAQGITDSFMEETPPLIPTQLIESVAAFGIFFYLCWRLRD
                     AAKLPAGFFMTRFLVLYAVFRFLIEFLRADLRGGFLFLSTAQWISIVLLAVLWIVGRR
                     SRVLTGLAVLTVPLLVASPLMALPPEKQLQHYVAQHWSVREGLPHNTVADIARTPDGL
                     WWLASPAGLVRFDGARATVFGKEQIAGLDDSRFTRLAVGSDGALWAGTEGGDIVRLRK
                     GVFTRFAPPVGVPRGPVFSLVALSDEAWASVGGAAIRMIEGAAPAQSSPLGHVADILL
                     AADARGAVVAAAGNEMAPLGAERSERLIFSRLVGTAFSALAFAPDGALWVGTTAGEII
                     RFDGPTDTTGRPVRKPDGIAITTFAFDDAVVWAGTQGAGLLRVREGAVDLFAERHGLR
                     GQMVRALYRDADGALWVGTEEGLSRFSDGAATTYTTVDGLSSNLVYAILEDNDGTLWV
                     GTRGGGLNRFTDSRFTAFTTKEGLPSDLIGALALDSDGSVWVGTAGGLSAVRRGRVTE
                     PYTTDDGLAQNLVGAILRDREDRLWVGTVGGAVNRLQNNKKFRSYLPPRPPLTTLVSQ
                     IYEDRSGRLWIAAQFGLGLFSDGYLKIFDAAEGLVGRAALAIAEDGDMNLWVGTHRGG
                     LNLYRNGRFSALTALNGLPVEDVYAVLIDDDDRLWFSTDAGIWYAPRLALMAYLEKRG
                     PKPPLGMVGLTDGMKTLECTGGVQPAAIRSRSGLFYFPTLKGLVAIDPRKVPRPDEMP
                     RAYVAGLTAGDRTALPGADILLPVGTTAVSIDIGAVAAARTGKTQFRYRLNGGDGRWR
                     EGSDTTVRYSELAPGAYEFEVRARLKGDAWGPVGRIAFTIAGERPFPFVTVLIVTLLT
                     AFFIGTAAVIIRKKRKKRDLPPPPAAPAPVPGPEKRVETDPVEQDDVDATAGEDIFLA
                     HEEEVPDPTPVVDPGDRPRQRYERSRLDDDTAGRLVAMILDHLDREKPYKDPDITLAR
                     LADRIGLTPHLLSQLLNDRLNKNFNHFINDYRIDEVKKLLDTPSNRDKLLAIAYDAGF
                     RSKTTFNTMFKRSTGKTPSEYRRSLDGGEIIDSDDMPE"
     gene            4218..4694
                     /locus_tag="PKH10_04040"
     CDS             4218..4694
                     /locus_tag="PKH10_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27331.1"
                     /translation="MWGTREKMLVDRLEPVAASMGLTIIELELPHNTGGFLRIFLDGR
                     DGRSVTVDDCARFSPVASSFLDTQEGFDFRYHLEVSSPGLERPIRRWEEVPRFIGTRV
                     TVMLTEKVEGRKKIIGTLTACDTARDSFTILAEDGATVVVPRGMVKRMHVIWEGDK"
     gene            4694..6301
                     /gene="nusA"
                     /locus_tag="PKH10_04045"
     CDS             4694..6301
                     /gene="nusA"
                     /locus_tag="PKH10_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNT27332.1"
                     /translation="MDFNIKELIDQLVKIKGIERSVIIEIIKDALYKAVQKKLGSEAD
                     IDIIYDDATGELQAFYFREVVEKVEEEDIHISLEDAHELNPDAVVGDTLGIKMEATEL
                     GRIAAQVAKQVILQKIKAIESDNTFNEFKDRKNEIVTGTVRRYERGGLVVDIGKAEAF
                     LPFNQQIPSDKFKVNERIKALIFDVVKTLQGCNIILSRVNTLFLVKLFEIEVPEIADG
                     IVKIEGVARDPGHRSKIAVRSIDRDVDPVGACVGMKGSRIQNIVHELSGEKIDIVPWD
                     EDPVRYICNTLSPVDVSQIIIDEEEHSMDVIVSDEQLSVAIGRGGENVRLASQLTGWS
                     IDIQSESQMQMMLEEARRRLLFIDGVDESMADSFIKLGYTTLEDITTSTPETLSEIPN
                     VTTERAQSIIAQAKEMLEKGLDAVEMDKELEEKLNVPVATLKGVNEELAAYLSDRGYH
                     TLADVQSEADTESFAKNAELSLRRARQIRYSLQTHIDELNGKTVERRTGVLDADSFFD
                     FSKIEQPTEGQEEKKEEKDEKEGGGDK"
     gene            6273..6893
                     /locus_tag="PKH10_04050"
     CDS             6273..6893
                     /locus_tag="PKH10_04050"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HNT27333.1"
                     /translation="MKKKAAATNKGPSVRSCVACGEKGRKETLVRFVLLNDVVSPDWR
                     GTLEGRAVYACPTPDCIRKLYAAKRLPDAFLQGVPRFAVPREEILAWIRGQAVASLTH
                     FLSLARKSGVLTTGQNTIADAMERDGKAPAAVLMTVDTVDRTVREVTRLLPAGTPILR
                     WGDQASLGAAIGTRPVGVVALAASPITERIRHYGAMINLFSLSQEH"
BASE COUNT         1475 a         2109 c         2186 g         1179 t
ORIGIN      
        1 cggcaagaag attggcgatg gcgctccgtg cctgctggcg ggccacccac tcttcgatcc
       61 ggggcaccga gaggtatgcc acgatggcga tgagaacgag caccacgacc atctctatca
      121 gcgtgaagcc cttgcgagtt ttctgacaca tgagcacacc tcgctcgttc atgggtccat
      181 tacaactcta aaaaaaataa aaatcgtgcc gccttataat actaaaccat ataaatagag
      241 gggcggcgtt cgggattata aaactgaact tgtaactatt tgatcattct ggcatatcgt
      301 cgctatcgat tatttctcca ccatcgagcg agcgacggta ttccgatggg gtttttccgg
      361 tggatctttt gaacatggta ttgaaggtgg tcttcgagcg gaacccggcg tcgtaggcga
      421 tggcgagcag tttatcgcgg ttcgacggcg tatcgagcag ttttttgacc tcatcgatgc
      481 ggtagtcatt gatgaagtgg ttgaaattct tatttaaacg gtcattcagg agttgcgaga
      541 gcaggtgcgg cgtaagcccg atgcggtcgg cgagccgcgc caaagtaatg tccggatctt
      601 tatagggttt ctctctgtca agatggtcga gtatcatcgc gacgagccgt cccgccgtat
      661 cgtcgtcgag ccggctccgc tcgtaccgtt gccgcggacg gtcgcccgga tcgacaacgg
      721 gagtcggatc cggcacctct tcttcgtgag cgaggaagat gtcctcccct gccgtcgcgt
      781 ccacatcgtc ttgctcgacg ggatccgtct cgaccctctt ctccggtccg ggaaccggcg
      841 ccggtgccgc ggggggcggc gggagatctc tcttttttct tttcttccgg atgatcaccg
      901 cggccgtgcc gatgaaaaaa gccgtcagca gcgtcacgat caggaccgtg acgaagggga
      961 acggccgttc acccgctatg gtgaaggcga tgcggccgac cgggccccac gcatcgcctt
     1021 tcagccgcgc acgcacctcg aattcatagg cgcccggcgc gagttcggag tatcggaccg
     1081 tcgtgtcgga cccttcgcgc caccggccat caccaccgtt caggcgatag cggaactgcg
     1141 ttttaccggt gcgcgccgcc gcgaccgcgc cgatatcgat ggaaacggcc gtcgtaccga
     1201 caggcagaag gatgtcggcg ccggggagcg ccgtccgatc gccggcggtg agtccggcca
     1261 cataggcgcg cggcatctcg tcgggtcgcg ggaccttgcg cgggtctatc gccacgagcc
     1321 ccttgagcgt cgggaagtag aacaggccgc tccgcgaacg gatggccgcc ggctggaccc
     1381 cgccggtgca ttcgagcgtc ttcatcccgt cggtaagccc caccatgccg agaggcggct
     1441 tggggccgcg tttttcaaga tatgccatca acgcgaggcg cggggcatac cagatgccgg
     1501 cgtcggtcga gaaccagagc cggtcatcgt cgtcgatgag caccgcatag acatcctcca
     1561 ccggcaggcc gttcagcgcc gtgagtgcgg agaagcggcc gttacggtac aggttgagcc
     1621 cgccgcggtg ggtgccgacc cagaggttca tgtcgccgtc ctcagcgatg gcgagcgccg
     1681 cgcggccgac cagcccctcg gcggcgtcga atatcttgag atacccgtcg ctgaacagcc
     1741 cgagcccgaa ctgcgcggcg atccacaggc ggccggagcg gtcctcgtat atctggctga
     1801 caagcgtcgt gagcggtggc cgcggcggca ggtaggacct gaattttttg ttgttctgca
     1861 ggcgattcac cgcgccgccg accgtaccga cccagagacg atcttcccga tcgcgcaata
     1921 tcgcgccgac gaggttctgc gccagaccgt cgtcggtcgt gtagggctcg gtcacccgtc
     1981 ccctccgcac cgcggacaga ccgccggcgg tcccgaccca gaccgatccg tcactatcga
     2041 gcgcaagcgc cccgataaga tcgctcggca atccctcttt cgtggtgaac gctgtgaagc
     2101 ggctgtcggt gaaccggttg agcccgccgc cgcgcgtacc gacccacaag gtcccatcgt
     2161 tatcctcgag gatcgcgtag acgagattgc tcgacagacc gtccaccgtg gtgtaggtcg
     2221 tcgccgcgcc gtcgctgaaa cgggagagtc cttcctcggt gccgacccag agcgcgccgt
     2281 cggcgtcgcg gtaaagggcg cgcaccatct ggccccggag cccgtgccgc tcggcgaaca
     2341 ggtccaccgc accctcgcga acacgcagga gccccgcccc ctgcgtcccg gcccatacga
     2401 cggcatcatc gaacgcaaaa gtggttatcg cgatgccgtc cggtttgcgc accggtctgc
     2461 cggtggtgtc ggtcggaccg tcgaaccgta tgatctcacc ggcggtggtg ccgacccaca
     2521 gcgcgccgtc gggcgcgaat gccagcgccg agaaggcggt accgacgagc cgagagaata
     2581 tcaaccgttc gctccgttcg gcgccgagcg gcgccatctc gttccccgcc gccgccacca
     2641 ccgcgccgcg ggcgtcggcg gcaagcagga tgtcggccac atgtccgagc ggcgaacttt
     2701 gcgcgggggc ggccccttcg atcatccgta tcgccgcacc gccgacggat gcccatgcct
     2761 cgtcggagag cgcgaccagc gaaaagaccg gcccgcgcgg gacaccgacc ggcggcgcga
     2821 aacgggtgaa cacccctttg cggagacgaa cgatgtctcc cccctcggtg ccggcccaga
     2881 gagcgccgtc gctaccgacg gcgagacggg tgaaacggct gtcgtcaaga ccggcgatct
     2941 gctctttccc gaacacggtc gcacgagcgc cgtcgaaccg gacgagcccc gcgggcgagg
     3001 caagccacca gagcccgtcg ggggtccgcg cgatgtcggc gacggtgttg tgcggcaggc
     3061 cttcgcgtac gctccaatgc tgggcgacat agtgctgaag ttgcttttcg ggcggcaggg
     3121 ccataagcgg ggacgcgacc agcaggggca ccgtcaggac cgccagaccc gtcaggacgc
     3181 gggagcgtcg cccgacgatc cacagcaccg cgagcaggac gatagatatc cactgcgcgg
     3241 tggaaaggaa caggaagccg ccgcgcagat cggcgcgcag gaactcgata aggaaccgga
     3301 ataccgcgta caggacgagg aacctcgtca tgaaaaagcc tgcgggcagt ttcgcggcgt
     3361 cgcggagccg ccagcaaaga taaaagaata tgccgaacgc cgccaccgat tcgatgagtt
     3421 gcgtggggat aagcggcggg gtctcctcca tgaaggagtc ggtgatgccc tgcgcgtaca
     3481 gttccgccgc ggccggcgta taggcgggaa aagagacgcc gaacgccttg gagggaagcc
     3541 cgaaacagca accggcgaag aaacagccga tgcgcgcgag agcgatgcca agcgcgaccg
     3601 gaaaggccgc gatatcgccg tagcagccgg tcggctcgcg ccagacgcgc cggatgaaca
     3661 gatagctgac gagatacgcg acgatgaagc cgccatagaa cacctgtccg gcgctgacag
     3721 ggttcacggc gacggcaagg aatgggacaa gcccctgctc gcggtatatc gtcgcatacc
     3781 gcgaccactg tccgtcgaac agaagatgga acagtttcga gcccgccaga ctcgcgacga
     3841 tggcgaccgc cgtaagcatc aacaggcgcc tgcttctcaa tgaaaaacga ggcgcaagga
     3901 aggcggtcag cgggaacgcg acgaaaaagg aaaagagcag catcgtgaaa tagggaccga
     3961 catacggaaa gcccagaaac tcgtataatg gcctgaagat gttgatattc atcgtgattt
     4021 caaacggttc gccctttcct tgtcacggcg gccatattag cggaacagcg cgcacaagtc
     4081 aatttaatgg ataaaatctt gctttttcgc tccccgagtg ctagatatcg cccgtgattt
     4141 cgttcattgc gcctgattct cagtggtgat agagagcggg atgtcgagtc ccgctgtttt
     4201 tttatgtgga ggtcgccgtg tggggaacac gggaaaaaat gctggtcgat cgactggaac
     4261 cggtcgccgc ctcgatggga ctcaccatca tcgaactgga actgccgcac aacaccggcg
     4321 gttttttgcg catattcctt gacggccgcg acggtcggtc ggtgacggtg gacgactgcg
     4381 cgaggttctc gccggtggcg tcgagttttc tcgacacgca ggagggcttc gatttccgct
     4441 atcatctcga ggtgagttcg cccgggcttg aacggccgat acgccgctgg gaagaggtgc
     4501 cgagattcat cggcacgcga gtgacggtca tgctcaccga aaaggtggag ggacgcaaaa
     4561 agatcatcgg cacgctgacc gcctgcgaca ccgcgcggga ttcctttacg atactggccg
     4621 aggatggcgc gacggtcgtc gtgccgcgcg gcatggtgaa acggatgcat gtcatttggg
     4681 aaggagataa gtaatggatt tcaacatcaa ggaactcatc gaccaactgg tcaagatcaa
     4741 gggcatcgaa cgctcggtca tcatcgagat catcaaggac gcgctctaca aggcggtgca
     4801 gaagaaactc ggctccgagg ccgacatcga catcatatac gatgacgcca ccggcgaact
     4861 gcaggccttc tatttccgcg aggtcgtcga aaaggtcgag gaggaggaca tacacatctc
     4921 gctcgaggac gcgcacgaac tgaatcccga cgcggtcgtc ggcgacacgc tcggcatcaa
     4981 gatggaggcg accgaactgg gccgcatcgc ggcgcaggtc gccaaacagg tcattctgca
     5041 gaagataaag gccatcgaaa gcgacaacac cttcaacgag ttcaaggacc gcaagaacga
     5101 gatagtgacc ggcaccgtgc gccgttacga gcgcggcggg ctcgtggtgg acatcggcaa
     5161 ggccgaggcg ttcctgccct tcaaccagca gataccctcc gacaagttca aggtcaacga
     5221 gcgcatcaag gcgctcatat tcgatgtcgt gaagaccctg cagggctgca acatcatcct
     5281 ctcccgcgtc aacacgctgt tcctcgtgaa actgttcgag atagaggtgc cggagatagc
     5341 cgacggcatc gtcaagatag agggtgtcgc gcgcgatccc ggccaccgtt ccaaaatagc
     5401 ggtgcgctcg atagaccgcg atgtggaccc ggtcggcgcc tgcgtcggca tgaaaggttc
     5461 gcgcatccag aacatcgtcc acgaactgtc gggcgaaaag atagatatcg tgccatggga
     5521 cgaggatccg gtgcgctata tctgtaacac actctcaccg gtcgatgtga gccagatcat
     5581 catcgacgag gaggagcatt cgatggatgt catcgtgtcg gacgaacagt tgtcggtcgc
     5641 catcggccgc ggcggcgaga atgtgcggct cgcgagccaa ctcaccggct ggagcatcga
     5701 catccagagc gagagccaga tgcagatgat gctcgaagag gcgcggcgcc gcctgctgtt
     5761 catcgatggc gtggacgaat cgatggccga ttctttcatc aaactgggct ataccactct
     5821 tgaggacatc acgacctcga cccccgagac cctctccgag ataccgaatg tcaccaccga
     5881 acgggcgcag tccatcatcg ctcaggccaa agaaatgctc gaaaaaggtc ttgacgccgt
     5941 cgagatggac aaggaactgg aagaaaaact caacgtgccg gtcgcgacgc tcaaaggggt
     6001 caacgaggag ttggcggcct atctgtccga ccgcggctac catacgctcg ccgatgtgca
     6061 gagcgaggcc gacaccgaat cgttcgcgaa gaatgccgaa ctgtcgctcc gccgcgcacg
     6121 acagatacgc tacagtctgc agacccatat cgacgaactg aacgggaaaa cggtggaacg
     6181 acgcaccggc gtactggatg ccgatagttt cttcgatttc agcaagatag aacagcccac
     6241 ggaagggcag gaagaaaaaa aggaagaaaa ggatgaaaaa gaaggcggcg gcgacaaata
     6301 agggaccgag cgtccgctcc tgcgtcgcct gcggggaaaa gggccgcaag gaaacgctgg
     6361 tgcggttcgt cctgctgaac gatgtggtgt cccccgactg gcgcggcacg ctcgaaggcc
     6421 gcgcggtgta cgcctgtccg acgcccgact gcatccgcaa actgtacgcg gctaaaagac
     6481 tgcccgacgc cttcctgcag ggagtgccgc ggttcgccgt cccgcgcgag gagatactcg
     6541 catggatacg cggtcaggcc gtagcgtcgc tgacgcactt cctctctctg gcacgcaaaa
     6601 gcggcgtgct gaccacgggc cagaacacga tagcggacgc gatggagcgc gacggaaaag
     6661 cgcccgcagc ggtgctgatg accgtcgaca cggtcgatcg caccgtgcgc gaggtcaccc
     6721 gtcttctgcc ggccgggacc ccgatactcc gctggggcga tcaggcgtcg ctcggcgcgg
     6781 cgatagggac acgaccggtc ggcgtcgtcg cgctggccgc ctcccccatc accgaacgaa
     6841 tccggcacta cggtgccatg atcaatcttt ttagtcttag tcaggagcat tgaatgcgca
     6901 tcagcgagat cgccaccgaa ctgggaaaga cctccaagga gatcatgga
//
LOCUS       DAOQUE010000090         6930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12037_length_6930_cov_4.361745, whole genome shotgun sequence.
ACCESSION   DAOQUE010000090 DAOQUE010000000
VERSION     DAOQUE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12037_length_6930_cov_4.361745"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PKH10_04055"
     CDS             <1..758
                     /locus_tag="PKH10_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNT27334.1"
                     /translation="RLRGAGDLPVVTGKGSSNDVLAATALPNEGLTIKNPRRLVRIEM
                     SVEKMGPDPAEACGQRVEKKGGARVITVEHPEKPCAGGVLPSDTAATIYEDKDAPGIQ
                     KTAARWRSISDRSVLVRKVVSFVYDHITDKNYRHGTLSASETLEKRAGDCTEHAVLAA
                     ALLKALGVPTRMAYGLVLSDDGRFFFHNWILVHTGTGWTNADPTYDQFPADAARLFMT
                     SGGAGAAEREDLSLSILNILRGARFTVTGFSHE"
     gene            751..1647
                     /locus_tag="PKH10_04060"
     CDS             751..1647
                     /locus_tag="PKH10_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27335.1"
                     /translation="MNNGRPFIILFLLLIGGPLFADQERGDIEMLSIDARNAAKGGGA
                     VFSTFGQFGDNPALGSVEQRFFVGSSYLFTESFSFAAADNKMGAFAGGLMYDRHNKLN
                     TVKTNSAFQIVDWFMFGFNLKFLSGPFYPLQREVLSLTADIGVLFNIEDIAYIGASFN
                     DFVPIGDNLPMRVTGQCEFNLYERMFLLNIAGSYHIDHREETVPNRRPQAAYADFSTG
                     FEFRYAWFSSQIGVHTSSFARDLTFDDLLKTVGVGFYKEREIKKNKKGEDEEEDGGGV
                     YAGVYITRTFVTFAVTFVWEPK"
     gene            complement(1650..2636)
                     /locus_tag="PKH10_04065"
     CDS             complement(1650..2636)
                     /locus_tag="PKH10_04065"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNT27336.1"
                     /translation="MATLATQYAGLTLANPFIVSSSSLTNTIEKIHRCEEAGAGAVVL
                     KSLFEEQIYEERVRAADPDLGMTYHTEAAAYIHQMSMDLGPEDYLGLIRGAKKAAKIP
                     IIASLNCVSSEWWQDYALRIQKAGADALEANIGFLTHDPNAESAEIESRILAIVQGLL
                     QGLKIPLIVKIGPFFTNPMALIRKIDRLGAAGVVLFNRFYQIDIDVDSLELRSGYLLT
                     PREDVTLALRWIALLSGRTKLSIAGSGGIYSGEDAAKYLLAGADAVQVCSALYKHRVE
                     HLRTMTTELSSWMQKHDLASLDKFRGKLSQSGHGDPAAWERHQYIKALVGLE"
     gene            complement(2686..3417)
                     /locus_tag="PKH10_04070"
     CDS             complement(2686..3417)
                     /locus_tag="PKH10_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27337.1"
                     /translation="MRAFCFLMALFLAGSLYPAEEAGWKVTEEVLVNGSKVIKHSHFS
                     DHYIKVVNEADTGSTETIIDLQNDKITLVNHFQKSFQVSKLSDYLKFAEGIATGLKSG
                     GYANPEKIEPKIVFEKRGTDTVGPWQTVRSLVKVDGKEYAEIWVAPELKNSPVLEYRK
                     KYAALLPETLVKYRALDEKIREQAAAEGLIVRMIKIPLNKKLPKIDQKMVSLAPVALT
                     PAMLLIPKDYLDKTITPGANTTGAK"
     gene            complement(3440..4165)
                     /locus_tag="PKH10_04075"
     CDS             complement(3440..4165)
                     /locus_tag="PKH10_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27338.1"
                     /translation="MPKKISSLLFCVSLFLSVSILCGAEKEFSPVLTAKEKALPDTGT
                     AYVYVDHRTPRHIINDLRAKDVPVAFLFDQRQAAWLRDIIPLLKGTAPIIFVRDTPSD
                     MDLKRLRETIDFFNYEATLVHVRRNGTGKLPSLPMKHILTLDINTGKQGSRFVLRGAS
                     RPPETATLLSALDERDLAEDLSVARGMIFPGYRFRFLNIDEEVYPGTAGADRTILFSN
                     SEKNVQKFIEKQRLHATIPPATE"
     gene            complement(4175..5890)
                     /locus_tag="PKH10_04080"
     CDS             complement(4175..5890)
                     /locus_tag="PKH10_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27339.1"
                     /translation="MERMMNLTALFLSFILFTFACSEGTKKTNDNSALGDNDDLLGDS
                     DDLAVNDADFAATCGNGFVDGGEPCDGNTEFCIAIDPVLYNGGKAKCLADCSGWDTAT
                     CDEVPHECGNNVKEGPEACDGDTKNCVDIDANKYSGGKAKCLTDCSAYDTATCDEKLP
                     VCGNNEVETGEICDGGTMSCVSLGAYIGGTAACKNDCSAWDTATCITEEMCGNDVLNT
                     GETCDKGTNSATKIKNCVEIDPYAYSGGKAKCNTTCDGWDTATCEELPIDNDIVPTDD
                     AVTDNDNEVPDTDSSCIDACSPQGSERCFNETVQTCAMGWEGCLIWSNTTYCYLNTPA
                     QICEFSNGDAYCAYDCTSECTSGDRRCNPTYIDQVDDCVQGEDGCYYWAYMEDCAYWG
                     MICVDPGVAGCDYESTEVIDTIGSSANYYTYGDRLKGNYFSCTSSRTLTEIETYMSFN
                     GYLDITYAIYSSSSQDGTYSLLSQKTVSSNSGSGTGNWYSSGTVSVSLTSGYYYFIGV
                     FFGTTDDISYPDPTYYAAASLNASPLAFGSHLGGGESSSYASLPSTISSIDVTYGYYM
                     RLTTE"
     gene            complement(5975..6538)
                     /gene="rsmD"
                     /locus_tag="PKH10_04085"
     CDS             complement(5975..6538)
                     /gene="rsmD"
                     /locus_tag="PKH10_04085"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNT27340.1"
                     /translation="MPIRITSGSLKGRLLAVPRGAVIRPTGEKIRAALFNTLYALADF
                     GNRCFLDLCSGSGAVGIEAVSRGFRQAIFVDKDVKTLTHLRRQVEFLGITSQTLFLPA
                     DIFSISWADRLSLPVDVIFMDPPYAMGDSVAPLIRRCIERSIIAPDGIIVAETDRELP
                     EQLMGWSRRGKRYGETFLSFYRHHEKP"
     gene            complement(6553..6843)
                     /locus_tag="PKH10_04090"
     CDS             complement(6553..6843)
                     /locus_tag="PKH10_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT27341.1"
                     /translation="MAQKPAKKKPGNGEFNAFVAEGNRAIGEQRFSDAIHAYKDALKI
                     KPRAGKVYKFLGIAYASTGNAAGACENYRKYISLVPDAPDRAQVEDLIAACK"
BASE COUNT         1389 a         1937 c         2025 g         1579 t
ORIGIN      
        1 atcgtctccg tggtgccggt gatctgccgg tggtgaccgg aaagggctct tcgaacgatg
       61 tgctggcggc gacggctctc ccgaacgaag ggttgacgat caagaatccg cggcgccttg
      121 ttcggatcga gatgagcgtc gaaaaaatgg gacccgatcc cgcagaagcg tgcgggcaac
      181 gggttgaaaa aaaaggaggg gcccgcgtca tcacggtcga acatcccgaa aagccctgcg
      241 ccggcggtgt cttgccgagc gataccgctg cgaccattta cgaggacaag gacgctcccg
      301 gcattcaaaa gaccgcggcg cgctggcgtt cgataagcga ccggagcgtt ctggtcagaa
      361 aggtcgtctc tttcgtttat gatcacatca ccgacaagaa ctatcggcac ggcacacttt
      421 cggccagcga gacgctggag aaaagggccg gtgactgcac cgaacatgca gtgctggccg
      481 cggctctcct caaggcgctc ggggtcccga cgcggatggc gtacggactc gtcctctccg
      541 atgacggccg attctttttc cacaactgga tattggtcca caccggcacc ggttggacga
      601 acgccgaccc gacctacgac cagttcccgg ctgacgcggc gcgactgttc atgacgagcg
      661 gcggcgccgg cgccgcggag cgggaggatc tttcgctctc catcctcaac atccttcgtg
      721 gcgcccgttt tacggtcacg gggttctccc atgaataacg gcaggccgtt catcatactc
      781 tttctcctcc tgataggggg cccgctgttc gccgatcagg aacggggcga tatcgaaatg
      841 ctttccatag atgcccgtaa tgcggcgaaa ggtggcggcg cggtcttcag caccttcggg
      901 cagttcggcg acaatccggc tctcggttcg gtggaacaga gattcttcgt cggctcttcc
      961 tatctgttca ccgaatcgtt ctctttcgcg gccgccgaca acaaaatggg cgcgttcgct
     1021 ggcggtctta tgtacgaccg tcataacaag ctgaacacgg tcaagaccaa cagcgccttc
     1081 caaatcgtcg attggttcat gttcggcttc aatctcaagt tcctttccgg ccccttctac
     1141 cccctgcaac gggaggtact gagtctcacc gccgatatcg gcgtcctctt caacatagag
     1201 gacatcgcct atatcggcgc ctcgttcaac gatttcgttc ctatcggcga caatctgccg
     1261 atgcgggtga ccgggcagtg cgagttcaac ctatacgaac ggatgttcct gctcaatatc
     1321 gccgggtcct accatatcga tcatcgcgag gagacggtac ccaaccggcg tccgcaggcc
     1381 gcttacgccg acttttcgac cgggttcgag ttccggtacg cgtggttctc ctcgcagatc
     1441 ggcgttcaca ccagttcgtt cgcgcgcgac ctcaccttcg acgatcttct caaaacggtg
     1501 ggggtcgggt tttacaagga acgcgagata aagaagaaca agaaaggcga ggacgaagag
     1561 gaggacggtg gcggtgtcta tgcgggggtc tacatcaccc gcaccttcgt tactttcgcc
     1621 gtcacctttg tctgggagcc gaagtagcgc tactccaatc cgacgagcgc cttgatgtac
     1681 tgatgtcgtt cccatgccgc cggatcaccg tgcccgctct gggagagttt gccgcggaac
     1741 ttgtcgagag acgccagatc atgtttctgc atccatgagg agagttccgt cgtcatggtc
     1801 ctgaggtgct cgacccggtg tttataaagg gccgaacaga cctgtacggc gtcggcgccg
     1861 gccaacaggt atttcgccgc atcctcgcct gaatagatgc cgccagaacc ggcgatggag
     1921 agcttcgttc gtccggaaag gagcgcgatc cagcgcaggg caagcgtcac atcctcccgg
     1981 ggcgtaagca gatagccaga ccggagctcg agcgaatcca catcgatgtc tatctggtag
     2041 aaacgattga agaggacgac cccggccgct ccgagccggt ctatcttccg tatcagggcc
     2101 atcgggttgg tgaagaatgg cccgatcttg acgatcaacg gtatcttcag tccttgtaaa
     2161 aggccctgca cgatagccag gatgcgtgat tcgatctcgg cgctttcggc attgggatca
     2221 tgcgtcagga agccgatgtt cgcttccagc gcgtcagccc cggccttctg tatccggagc
     2281 gcgtagtcct gccaccactc ggaggatacg cagttgagac tcgcgatgat cggtatcttc
     2341 gccgcttttt tagcgccgcg gataagcccc agataatcct ccgggccgag atccatactc
     2401 atctgatgga tataggcggc cgcttccgtg tggtaggtca tgccgagatc cgggtctgcg
     2461 gcgcgcaccc gctcctcgta gatctgttcc tcaaaaagcg atttgagaac caccgcgcca
     2521 gcgcccgcct cttcgcaacg gtgtatcttt tcgatggtgt tggtgaggct ggagctgctt
     2581 actatgaacg ggttcgcaag cgtcaatccc gcatactgcg tcgccagtgt ggccatggcc
     2641 ggatcctccg taagatcata tgccttaaaa cgtcctatgg tcgagctact tcgcgccggt
     2701 ggtgttcgcc cccggggtga tggtcttatc aaggtaatct ttcgggatga ggagcatggc
     2761 cggcgtcagc gccacggggg caagcgagac catcttctga tcgatcttgg gcagtttctt
     2821 gttgaggggg atcttgatca tacggacgat aagcccttcc gctgccgcct gttcacgtat
     2881 cttttcgtcg agggcgcgat attttaccag cgtttcgggg agtaatgcgg cgtatttctt
     2941 tcgatactca aggaccgggc tgttcttaag ttcgggtgcg acccatatct cggcatattc
     3001 cttcccgtca acttttacga gagaacgaac ggtctgccat gggccaacgg tgtctgttcc
     3061 ccttttctcg aacacgatct tcggttcgat cttctcaggg ttggcgtacc cgccgctttt
     3121 gagtccagtt gcgatgcctt cggcgaactt cagatagtcg cttagttttg atacctgaaa
     3181 gctcttctga aaatgattga ccagcgttat tttgtcgttc tgcaggtcaa tgatggtttc
     3241 ggtgctaccc gtgtcggcct cgttgaccac ctttatgtag tggtcgctga aatgggaatg
     3301 tttgatcacc ttgctaccgt tgaccaaaac ctcttcggtg accttccacc cggcttcttc
     3361 ggcgggataa agcgatcccg cgagaaaaag agccatcaag aaacaaaagg cgcgcatggc
     3421 gttctccctc tttatccatc tattccgttg ccgggggtat tgtagcgtgt aatcgctgtt
     3481 tctcaataaa tttctggaca tttttctcgc tgttggagaa aaggatggtc cggtcggcac
     3541 cggcggttcc cgggtagacc tcttcgtcga tattcaaaaa acgaaagcga taaccgggaa
     3601 agatcatgcc ccgcgctaca gaaagatctt ccgcaaggtc acgttcatcg agtgccgaaa
     3661 ggagtgtggc tgtttcggga ggccgggaag cgccgcgtag gacgaaacgg gagccctgct
     3721 taccggtgtt gatatcgagc gtcaggatat gtttcatggg gagggagggg agtttccccg
     3781 ttccgttccg cctgacatga acgagcgtgg cctcataatt gaagaaatcg atcgtttccc
     3841 ggagtctttt caaatccata tctgaagggg tgtcgcgtac gaagatgatc ggcgccgtac
     3901 ctttgagcag gggaatgata tcgcggagcc aggccgcctg gcgctggtcg aaaaggaagg
     3961 cgaccgggac atcctttgcg cggaggtcgt tgatgatatg tcggggggtt cggtgatcca
     4021 catagacata cgcggtgccg gtgtccggta gcgccttttc cttcgccgtc agcaccggag
     4081 agaactcttt ctcggcaccg cacagtattg atacggaaag gaagagggat acgcagaaga
     4141 gcaaggagga gatctttttt ggcaagttcc gctcctactc ggtggtcaat cgcatatagt
     4201 agccgtaggt cacatcgatt gaggaaatgg tgctgggaag acttgcgtaa gagctcgatt
     4261 cgccgccgcc aaggtgcgat ccgaatgcga gcgggctggc gttaagcgat gcggcggcgt
     4321 aataggtggg gtcgggatag gagatatcat cggtcgtccc gaagaacacg ccgatgaaat
     4381 aatagtaccc gctggtcaaa ctcaccgata cggtgcccga actgtaccag ttgccggtgc
     4441 cgctcccgga gttggaagag acggtctttt gcgaaagcag actgtaggta ccgtcctgag
     4501 aggaggaaga atagattgcg taggtgatgt cgaggtagcc gttgaaagac atgtaggtct
     4561 ctatctcggt cagcgtccgc gaggaggtac aggagaaata gttccccttc aatcggtcgc
     4621 cgtaggtgta gtagttcgcg ctcgagccga tggtgtcgat cacttcggtc gactcgtagt
     4681 cgcaccccgc cacgccggga tcgacgcaga tcatccccca ataggcgcag tcctccatat
     4741 aggcccagta gtagcaccca tcctcgccct gtacgcaatc gtccacctga tcgatatagg
     4801 tcggattgca gcgtcgatcg ccgctggtgc actcgctggt acagtcgtag gcgcagtagg
     4861 catcaccgtt cgagaattcg catatctgcg ccggggtgtt gagataacag taggtggtgt
     4921 tcgaccagat aagacatcct tcccagccca tggcgcaggt ctggaccgtt tcattgaaac
     4981 accgctcgct tccctgcggc gaacaggcgt cgatgcacga ggaatcggtg tcgggcacct
     5041 cgttgtcgtt gtcggtcacg gcgtcgtcgg tcggtacgat gtcgttgtcg atcggcaatt
     5101 cctcgcaggt ggcggtgtcc caaccgtcgc aagtagtgtt gcacttggcc ttgccgcccg
     5161 aataggcgta cgggtctatc tcaacgcagt tcttgatctt cgtcgcgctg ttcgttccct
     5221 tgtcgcaggt ctcaccggtg ttgagcacat cgttcccgca catctcttcg gtgatgcagg
     5281 tggcggtgtc ccaggcggaa cagtcgttct tgcaggcagc ggtgccgccg atgtaagcgc
     5341 cgagtgatac gcaactcatg gtcccgccgt cacagatctc ccccgtttcc acctcgttgt
     5401 tgccacagac cggtaatttc tcatcacagg tggcggtgtc ataggccgag caatcggtaa
     5461 gacatttcgc cttcccgccg ctatatttat tcgcgtctat atcgacgcag ttcttcgtgt
     5521 cgccatcaca ggcctcgggt ccctccttga cattgttgcc gcattcgtgc gggacctcgt
     5581 cgcaggtggc ggtgtcccag cctgagcagt cggcgaggca cttcgctttc ccgccgttgt
     5641 aaagcacggg gtctatggcg atacagaatt cggtgttccc gtcgcagggt tcgccgccgt
     5701 cgacaaaacc gttaccgcag gtcgccgcga aatcggcgtc gttcaccgcg aggtcgtcac
     5761 tgtcgccgag cagatcgtcg ttgtcgccaa gggcgctgtt gtcgttggtc ttcttggtcc
     5821 cttcgctaca ggcgaaggtg aaaagaatga acgaaaggaa cagggccgtc aggttcatca
     5881 tgcgttccat ggcgtttcct cccacaataa aaaacggtga aagtatactc cgatgcgctc
     5941 tgcggagcaa ggaaatcttc aaaagggggc gtcttcatgg cttttcgtgg tgccgataga
     6001 aagagaggaa tgtctccccg tacctttttc ccctgcgtga ccagcccatg agctgctcgg
     6061 gcagttcgcg atcggtctcg gcaacgatga tgccgtcggg cgcgatgatg ctccgttcaa
     6121 tgcagcgtcg tatcaagggg gccacactat cgcccatggc gtacggaggg tccatgaata
     6181 tcacatctac gggaagcgac aaacgatccg cccatgagat cgaaaagata tcggcaggca
     6241 aaaaaagtgt ttgcgaggtg atacccagaa attccacctg tctgcgcagg tgagtcagtg
     6301 tctttacgtc cttatcgacg aatatcgctt gcctgaaacc ccggcttacc gcttctatac
     6361 cgacggcgcc gctgccggaa cagagatcga gaaagcacct gttcccgaaa tctgcgaggg
     6421 catacaatgt gttaaaaagc gccgcccgta tcttttctcc ggtcggtcgt atcacggcac
     6481 cacggggcac ggcaagcaag cggcctttca gggaccctga ggtgatgcgt atgggcataa
     6541 gcggaagccc cgctatttac aggcggcgat gagatcctcg acctgcgcgc gatcgggtgc
     6601 gtccgggacc agagagatat atttccggta gttctcgcat gcgccggcgg cgttcccggt
     6661 cgaggcatag gcgataccga ggaatttata aaccttaccc gcgcgcggct ttattttcaa
     6721 tgcgtccttg taggcatgta tggcgtcact gaaccgttgt tcgccgatcg cccgattccc
     6781 ttcagcaaca aaggcattga actccccatt ccccggtttc tttttggcag gtttctgcgc
     6841 cacgggcttc ggggcgctct ttacaggttc cgggcttgga gacgatggcg taggtttgat
     6901 atccggtgct ggcgccggag caggctccgg
//
LOCUS       DAOQUE010000091         6911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12083_length_6911_cov_4.140753, whole genome shotgun sequence.
ACCESSION   DAOQUE010000091 DAOQUE010000000
VERSION     DAOQUE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12083_length_6911_cov_4.140753"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..1902
                     /locus_tag="PKH10_04095"
     CDS             328..1902
                     /locus_tag="PKH10_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT27342.1"
                     /translation="MSLADIIVYFGGTGLGLAFVYIIWGYLKGLKRSPGDMWILFAYL
                     LVTYVAYSSQNVSLTLWLSADCGLGDIAAGTYITTWSIMLSMVGMVAGALVDTIGIRR
                     TLFFSVIFLLIARVSMVFLTNPLIVFIFGFVPYAIGFAIVSPVISVAIKRFTTKESAA
                     LGFGLFYVLMNGAFAVGGVFFDKVRGAYALKDAAGKIVNDNAGTMLFGAHFSTYQMLY
                     LFVLILTVVSVGLIFFVRSGVELDDDGNVVIKPLIDHGSGLAAVKSALVKTGQMIKSV
                     VSEKYFWIFITIITLTIFVRMVFFQLQYTFPKYGLRVLGLDAKIGTIYGVLNPVLIIF
                     LVPVVALLTKKISSYKLLVVGSIVSASSCFIVMIPPAAVSWLTDTVWGELVFVNWLGM
                     APTHEALMQNPPVPEYWPFIIFILVFTIGESIWSPKLMQFTAEIAPKGKEGTYLALSL
                     LPFFLAKLIAGPMSGILLNAYVPVGPNKEILGPYPDHSMIWFWIGAMAIITPIGLLLM
                     KGTFQKREESAAAAGH"
     gene            1974..3647
                     /locus_tag="PKH10_04100"
     CDS             1974..3647
                     /locus_tag="PKH10_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT27343.1"
                     /translation="MNAVETTLRAADIIVYAGLVGLAVAFFWIVWSYAKGLKDSPRDM
                     WILFTYKIIQYVAYAAMNPILILWLSKECGLGDIAAGNYIMAWSILLSLVGMVSGALV
                     DTIGIKRTLIFSVIFLVIARFFMSFVTHPVLIFFIGFVPFAIGFAMVAPVISVSIKRF
                     TTKEGATLGFGLFYVIMNAAYAIGGWLIDYFRTVFVERDAAGKIINENFGTMLFGMHF
                     STYQLLFILALALTVLSVACIFFIRDGIEVEEATDEQGKKSSKIVVKPSQDHGSGLAA
                     IKSAFLTMYGRIKSAVSENYFWIFILILTVTLFVRFIFFHLHYTFPKYGIRVLGEGAK
                     IGNIYGVLNPALIIFLVPLFAAITKKWRSFTMLVVGSAISSLSCFIAVIPGVYFSGLT
                     DSVLGEIVFIKWLGMAPDMPTLLANPPVPEYWSLIVFFTVFTIGEAIWSPRLMQFTAE
                     IAPKGKEGTYIALAVLPFFLAKFVVGPMSGMLVNAYTPMVDKIDPATGAVVLDAAGKA
                     VQVVGDLSQHYMVWVWIGGMAIVTPIGLLLLHGIYKKHMEEKTEPAKAH"
     gene            3740..5602
                     /locus_tag="PKH10_04105"
     CDS             3740..5602
                     /locus_tag="PKH10_04105"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT27344.1"
                     /translation="MKTLNFIAIFLVVTAVSCGTKVGGDPGSVGPDIDTVTDKTLDNY
                     GEEQIGYKNSSAARAAEEEKEQEDINGGSAPAEDESPGDGSYSDSATVSDQSSPVSDT
                     DNAWYPDEAVEEEPDEDMLLTDEEPLRVKSEEVTPILENPYFETATVDRSTFSIDVDT
                     GSYSLVRGYLKNGELPPASSVRIEEMINYFHMRYKQPEGDTPFRLYTELSTCPWNPKH
                     KLLMLGVQGQEVALADQPPANLVFLIDVSGSMTYSENKLPLLKKAFRMMASQMRDIDR
                     VAIVTYAGKEALVLDSTPGSEKQKIYAALDNLEGGGSTNGSGGIQLAYEVAAENFLPE
                     GNNRVILATDGDFNVGITDQNELVNFIAEKRETGVFLSVYGFGEAWDGGNYRDEQLEQ
                     LADNGNGIYFYIDGPEEARRAFLNGLSGSLLTIAKDVKLQVAFNKEQVKAYRLVGYEN
                     RVLANDDFNNDTVDAGELGAGLSVTAFYEIIPATSGEAVPEPQPGTVEEPDTDAEVSD
                     DDTILDETPVTGDTFVNVRIRYKDPDADTSQLLEHPLDADDIDRTVPSLKFLFAAGVT
                     EFSMLLRHSQYLPTRDIDELLKLAKAALPADAEGAIAEFIDLVELAKDHLDGQP"
     gene            5698..>6911
                     /locus_tag="PKH10_04110"
     CDS             5698..>6911
                     /locus_tag="PKH10_04110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT27345.1"
                     /translation="MTPALAFHLLTAVVAVIIAVIVVVYIKGLRASPRDLWLLYGTKV
                     LEYSAYAMINMTIVLYLTKNFGMGDVAAGTYFSVWSILINFFTIVIGAVTDTIGIKRT
                     LLLGTITLVITRFILALSGDIYTATVFGFMPYAFGLAVMAPVISVGIKKMTTKETAAM
                     GFALFYTLMNVGYAIGGWLWDKIRIGVAAGGGSWTVPGLGAALAPYEAIFFAGFLLTL
                     PSLLFLSLLRDGVELDDKGQVVFKPQPKLPGGIFTVAKNTAVNALTGTAKSLKSTWSD
                     PFFYKFLLIIFIVVFVRLVFFHLNSMTLPKYGLRLFGPGAKIGALYGVLNPVLVVILV
                     PLVGYFFKKVPSYRMLLIGTTVSALACFIGVMPGAWFEGLNGTLLSGIVFTEWLGVAG
                     DPHPAYWPLLIF"
BASE COUNT         1307 a         2191 c         1935 g         1478 t
ORIGIN      
        1 gaggtatcgc cctgatggac gccgctttcg tagggctggc tctgattgtc ggcgagcagc
       61 gacttggaga cgatgaagta atcgaacgcc ccctcgtagg tgaaggcggg ctgatcgtag
      121 aaggccccgg cggtcgcgct gatcatcagc agggaggcga tggtgacgat ctttttcatg
      181 gcacggtctc ctgacggtaa tgaccgtcgc tactgtacga ccccgtcacc gtgaagtcaa
      241 gggcgagatt ttaggccaaa aaactttctt tgccccgccg tctcgataca atgcccccga
      301 ttttccacat gccttggagg ttctcttatg agtcttgcgg acatcatcgt ctatttcggc
      361 ggcaccggtc ttggtctcgc attcgtctat atcatctggg gctacctgaa gggactcaaa
      421 cggtcgccgg gcgacatgtg gatactgttc gcctacctgc tggtgaccta tgtcgcctac
      481 tcgtcgcaga acgtatcgct gacgctgtgg ctctcggccg actgcggatt gggtgacatc
      541 gcggcgggga cctacatcac cacctggtcg atcatgctct cgatggtcgg gatggtggcg
      601 ggtgcgctgg tcgacaccat cggtatccgg cggaccctct ttttcagcgt catattcctg
      661 cttatcgccc gtgtgtcgat ggtctttctc accaatccgc tgatcgtttt catcttcggt
      721 ttcgtcccgt acgccatagg tttcgcgatc gtctcgccgg tcatatcggt cgccatcaag
      781 cgtttcacca ccaaggagag cgcggcgctc ggcttcggcc tgttctatgt gctgatgaat
      841 ggcgcgttcg ccgtcggcgg ggtctttttt gacaaggtac gcggcgccta tgcgctgaaa
      901 gacgcggcgg gaaagatagt caacgacaac gccgggacga tgctgttcgg ggcacacttc
      961 tcgacctatc agatgctgta tctgttcgtg ctcattttga ccgttgtgag cgtcggactc
     1021 atcttcttcg tccgttcggg ggtcgaactc gacgacgacg ggaatgtggt gatcaagccg
     1081 ctcatcgatc acggctcggg actcgctgcg gtcaaaagcg cgctggtcaa gacgggacag
     1141 atgataaagt cggtcgtttc ggagaagtat ttctggatat tcatcaccat catcaccctg
     1201 acgatattcg tccggatggt ctttttccag ttgcagtaca ccttcccgaa atacggattg
     1261 cgggtcctcg ggctcgacgc gaagatcggg accatctacg gcgtcctcaa cccggtgctc
     1321 atcatcttcc tcgtaccggt ggtggcgctc ctcaccaaga agatatcctc ctacaaactg
     1381 ctggtggtcg gatcgatcgt gtcggcctct tcctgcttca tcgtcatgat cccgcccgcg
     1441 gcggtgtcgt ggctgaccga caccgtctgg ggagaactgg tgttcgtcaa ctggctcggc
     1501 atggcgccga cccacgaggc gctcatgcag aatccgccgg tccccgaata ctggcccttt
     1561 atcatcttta tcctcgtgtt caccatcggc gagtcgatct ggtcgccgaa actcatgcag
     1621 ttcaccgccg agatcgcgcc gaaggggaag gaggggacct atctcgccct ctctctcctg
     1681 ccgttcttcc tcgcgaaact catcgccggc ccgatgtcgg ggatcctgct gaacgcctac
     1741 gtgccggtcg gcccgaacaa ggagatactc ggtccctatc ccgatcacag catgatctgg
     1801 ttctggatcg gcgcgatggc gatcatcacc cccatcggcc tcctgctcat gaaggggacc
     1861 ttccagaaac gggaagagag cgccgccgcc gcaggccact gaacaaaaaa aactttactc
     1921 cgccgcccgg cgtcggtatc ttccggcgac actttatctg acgggagtca cgaatgaacg
     1981 ctgttgaaac gaccctccgg gccgccgaca tcattgtgta tgcggggctg gtcggtctcg
     2041 cggtcgcctt tttctggatc gtgtggagtt acgcgaaagg actcaaggat tcgccgcgcg
     2101 acatgtggat actgttcacc tacaagatca tccagtatgt cgcctacgcc gcgatgaacc
     2161 ccatcctcat cctgtggctc tcgaaggaat gcgggctggg cgacatcgcc gccggcaatt
     2221 acatcatggc gtggtcgata ctgctgtcgc tggtcgggat ggtctcgggc gcgctggtcg
     2281 acaccatcgg catcaagcgg acgctcatct tcagcgtcat cttcctcgtg atcgcccgct
     2341 tcttcatgtc gttcgtcacc catccggtgc tgatattctt catcgggttc gtgccgttcg
     2401 ccatcggttt cgcgatggtg gcgccggtca tatcggtctc catcaagcgc ttcaccacca
     2461 aggagggcgc gacgctcggc ttcggcctgt tctacgtcat catgaacgcc gcttacgcga
     2521 tcggcggctg gctgatagac tacttccgca cggtgttcgt cgaacgcgac gccgccggca
     2581 agataatcaa cgagaatttc ggtacgatgc tcttcgggat gcacttctcg acctaccagt
     2641 tgctgttcat actggcgctg gcgctcacgg tactcagcgt tgcctgcatc ttcttcatcc
     2701 gcgacggcat cgaggtggag gaggcgaccg acgagcaggg caaaaaaagt tccaagatcg
     2761 tcgtcaagcc gagccaggac catggttcgg gactcgccgc gataaaaagc gccttcctca
     2821 ccatgtacgg tcgcatcaag agtgccgttt cggagaatta cttctggata ttcatcctca
     2881 ttctcaccgt gacactgttc gtccggttca tcttcttcca tctccactac acgttcccga
     2941 agtacggcat ccgcgtgctc ggcgaagggg cgaagatcgg caacatctac ggtgtcctca
     3001 accccgcgct catcatattc ctcgtaccgt tgttcgccgc gataacgaaa aaatggcgct
     3061 ccttcacgat gctcgtggtc ggctcggcga tatcgtcgct ctcctgcttc atcgccgtta
     3121 tccccggcgt ctatttctcg ggactcaccg attcggtcct cggcgagatc gtcttcatca
     3181 aatggctcgg gatggcgccc gacatgccga cgctgctggc caacccgccg gtccccgaat
     3241 actggtcgct catcgtcttc ttcaccgtct tcaccatcgg cgaggcgatc tggtcgccgc
     3301 ggctcatgca gttcaccgcc gagatagcac ccaagggcaa ggaggggacc tacatcgcgc
     3361 tcgcggtcct gccgttcttc ctcgccaagt tcgtcgtcgg tccgatgtcg gggatgctcg
     3421 tcaatgccta caccccgatg gtcgacaaga tcgacccggc gaccggcgcg gtcgtactcg
     3481 acgccgccgg gaaggcggtg caggtggtgg gcgacctttc ccagcattat atggtctggg
     3541 tctggatcgg cggcatggcg atagtcaccc cgatcgggtt gctgctcctg cacggcatct
     3601 acaagaaaca catggaagag aagaccgaac cggccaaagc acactagcgc cgtctttttt
     3661 acctcgtctt taataaaatc gaccttcccg gaacaccata cccccggtcc gttgcatgaa
     3721 accgagagtg gaggaaacca tgaagacatt gaattttatt gctatttttc ttgtcgtgac
     3781 cgccgtttcc tgcgggacga aggtgggtgg cgaccccgga tcggtcggcc ccgacatcga
     3841 tacggtgacc gacaagacgc tcgacaacta cggcgaggag cagatcggct ataagaacag
     3901 ttcggccgcc cgcgccgccg aggaagaaaa ggaacaggaa gacatcaacg gaggaagcgc
     3961 ccccgccgag gacgagtcgc ccggcgacgg ttcctacagc gacagcgcca ccgtttcgga
     4021 ccaatcatcg ccggtctccg acaccgacaa tgcgtggtat cccgacgagg cggtcgagga
     4081 ggagcccgac gaagatatgc tcctgaccga cgaagaaccg cttcgtgtga agagcgaaga
     4141 ggtgacgccg atactcgaga atccttactt cgagaccgcg acggtcgacc gatccacctt
     4201 ctcgatcgat gtcgacaccg gctcctattc gctggtgcgc ggctacctga agaacggcga
     4261 actgccgccc gcctcatcgg tccgcatcga ggagatgatc aactacttcc atatgcgcta
     4321 caaacagccc gaaggcgata cccccttccg gctctacacc gaactgtcga cctgcccgtg
     4381 gaacccgaaa cacaaactgc tgatgctcgg cgtacagggg caggaggtgg cgctggccga
     4441 ccagccgccc gccaacctcg tgttcctcat cgatgtgtcg ggctccatga cctattcgga
     4501 gaacaaactg ccgctcctca aaaaggcgtt ccggatgatg gcctcgcaga tgcgcgacat
     4561 cgatcgcgtc gccatcgtca cctacgccgg caaggaggcg ctcgtcctcg attcgacccc
     4621 cggtagcgag aaacagaaga tatacgcggc gctcgacaac ctcgaagggg gcggctcgac
     4681 caacggatcg ggcggcatcc aactcgccta cgaggtggcg gctgagaact tcctgcccga
     4741 gggcaataac cgcgtcatcc tcgcgaccga cggcgatttc aatgtcggca tcaccgacca
     4801 gaatgaactg gtgaacttca ttgccgaaaa aagggagacc ggcgtattcc tgagcgttta
     4861 cggtttcggt gaggcgtggg acggcggcaa ctatcgtgac gagcagttgg agcaattggc
     4921 cgacaacggg aacggcatct atttctacat cgacggtccc gaagaggcac gtcgtgcgtt
     4981 cctcaacggg ctgtcgggct cgctcctcac catcgccaag gatgtgaaac tgcaggtcgc
     5041 tttcaataag gagcaggtta aggcctaccg gctcgtcggc tacgagaacc gcgtactcgc
     5101 caacgatgac ttcaacaacg acaccgtcga tgcgggtgaa ctcggcgcgg gcctgtcggt
     5161 caccgccttt tacgagatca tccccgccac gagcggcgag gcggtccccg agccgcagcc
     5221 gggcaccgtc gaggagcccg acaccgacgc cgaggtgagc gacgacgaca ctatcctcga
     5281 cgagacgccg gtcaccggcg acaccttcgt caatgtccgc atccgctaca aggatcccga
     5341 cgccgatacg agtcaactgc tcgaacatcc gctcgatgcc gatgatatcg accggaccgt
     5401 gccgtcgctc aagttcctgt tcgcggcggg cgtcaccgag ttctcgatgc tcctgcgtca
     5461 ttctcagtac ctgccgaccc gcgatatcga cgaactgctg aaactcgcca aggcggcgct
     5521 cccggccgac gccgaggggg ccatcgccga gttcatcgac ctcgtcgaac tggcgaaaga
     5581 tcacctcgac ggccagccct gagcatcttt ctcttttccg cacctccgat ttatgttgcc
     5641 tctctccctt ttctctgcta gcatccaccg accacaaccc tgtacacgga ggccgtcatg
     5701 acccccgcgc tcgcgttcca cctgctcacc gctgtcgtcg ctgtcatcat cgccgtcatc
     5761 gtcgtcgttt acatcaaggg tctgcgcgcc tcgccgcgcg acctgtggct cctctacggc
     5821 accaaggtcc ttgaatattc ggcctacgcg atgatcaaca tgaccatcgt cctgtacctc
     5881 accaagaact tcgggatggg cgatgtcgcg gcggggacct atttctcggt atggtccatc
     5941 ctcatcaact tcttcaccat cgtcatcgga gcagtgaccg acaccatcgg catcaaacgg
     6001 acgcttctcc tcgggacgat aacgctcgtc atcacccgtt tcatcctcgc cctttccggc
     6061 gatatctaca ccgcgacggt cttcggcttc atgccgtacg ctttcgggct cgcggtgatg
     6121 gcgccggtca tatcggtcgg catcaagaag atgaccacca aggagaccgc cgcgatgggg
     6181 ttcgccctgt tctacacgct catgaatgtg gggtatgcca tcggtggctg gctgtgggac
     6241 aagatacgga taggcgtcgc ggccggcggc gggtcgtgga ccgtgccggg gctcggggcc
     6301 gcgctcgcac cctacgaggc gatattcttc gccgggttcc tgctgaccct gccgagcctg
     6361 ctgttcctct cgctcctgcg cgacggggtc gaactcgacg acaagggaca ggttgtcttc
     6421 aagccacagc cgaaactccc cggcggtata ttcaccgtgg cgaagaatac cgcggtcaat
     6481 gccctgaccg gtaccgcaaa aagtctcaag agcacctgga gcgacccctt tttctacaaa
     6541 ttccttctca tcatcttcat cgtggtcttc gtccggctcg tctttttcca tctcaatagc
     6601 atgacgctcc cgaaatacgg cctgcgcctg ttcggtcccg gggcgaagat cggcgcgctc
     6661 tacggcgtcc tcaatccggt gctcgtcgtg atactcgtgc cgctcgtcgg ctacttcttc
     6721 aagaaggtac cctcctaccg catgctcctc atcggcacca ccgtctcggc gctcgcctgc
     6781 ttcatcgggg tgatgccggg cgcatggttc gaagggctca acgggacgct cctgtcgggc
     6841 atcgtcttca ccgaatggct cggcgtcgcg ggcgacccgc accccgccta ctggccgctc
     6901 ctcatcttca t
//
LOCUS       DAOQUE010000092         6899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12102_length_6899_cov_4.295733, whole genome shotgun sequence.
ACCESSION   DAOQUE010000092 DAOQUE010000000
VERSION     DAOQUE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12102_length_6899_cov_4.295733"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PKH10_04115"
     CDS             complement(<1..618)
                     /locus_tag="PKH10_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27346.1"
                     /translation="MMIPSRLLVLTILALLFSSCEKLADLVTEEAPQRYLILYTNDEH
                     GHIWEPEGREKAVILDEMWREEERDCAGCIVIRVSGGDNYTGSAISSIFKGRSAAEAM
                     HDIGYQIAAVGNHEFDYGMTEFDENRTASKIKYLSANIIGKGMKPVFPASHILDREGS
                     RLAFIGVTTEELRQVSFPANLKDLKTVQALNAVQREIRRLKGEADT"
     gene            744..1325
                     /locus_tag="PKH10_04120"
     CDS             744..1325
                     /locus_tag="PKH10_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNT27347.1"
                     /translation="MQGTSTEHIVIIAGIGAVLLRRHARVRNIVLRLNDDGDPVATVP
                     HWVPYGEAIDFVISRRDWITRQQRERAAAPAAPPPLPSEPLSITREEARPLLERRIAE
                     LARRHDLVVTRVTVRAQRTVWGSCAVDGGININWRTARLPDALRDYVILHELAHLIYR
                     SHGPRYWRTLDRLVGGDARTLDRELRRWPLAAL"
     gene            1359..2402
                     /locus_tag="PKH10_04125"
     CDS             1359..2402
                     /locus_tag="PKH10_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT27348.1"
                     /translation="MYDKNGENGIAAMTLLLEGLLLGLSLGVTCIATCAPVYAPILMQ
                     TERGTLGALWAVVLMSAGRFLSYAAVGVAVGAIGAAAAGTLPIPTIAAVSYLFIALFL
                     IYTALIRGRAEKHGNCPAGLMSRFAGNPFVAGLVTGFSVCPPFIGAITRGFDTGGALG
                     GMLLFIGFFVGTTLYFIPMAILPLLAHRRLIRIAGIFFSLCAAFWFTLLGLDHFLDLR
                     IKAEMYLEDAYLVDYSQAELTLVDATGDPALTERAARFFPHARAMTASAERYAETLAA
                     LAPATNVLLLVTTTPDESVRTLAIEKRLNIVWAIAPRTDAGTVELRDFLATYIIKARQ
                     SRGFLFRLPLDPR"
     gene            2502..3761
                     /locus_tag="PKH10_04130"
     CDS             2502..3761
                     /locus_tag="PKH10_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27349.1"
                     /translation="MKKMVFLFSLFAAIALNAEINRPVYETGYESYAPLGNTPVLSQF
                     VTFEDAYWLPSVLPELGKGWVYINNNGQYGGVIFPLLERKLFMHVFVEYDPNEANYTR
                     NSPRQLVEIMRGVSFDTLGNDEYIAPLSNIFGFGFAGDVGTLSWGINLKLYADQYSRK
                     TTTPATTATLSNSTFRLEFTPSISMKRDEWRMDAGFNLLGQWISNETSNQTFVYPYDY
                     AGNAEFAIYGRMMYDITKYTTLMGNLSFGYAGASDKLDNDHVSLDNTYLVLKGGALIK
                     PVERLTLGSNFSFYHAWQGLNRTTSPGGVTTKTEDSGNLLVFNFTQTADFKPCDWFTL
                     KAGIGKNIFVNVSTDKVIVPAGTSTDDDYDNNFDIGKFIGFAIDYKEFNFTAVMNFDV
                     FTEGPYLLTGRAWANPMAFVASLNYQW"
     gene            3836..4039
                     /locus_tag="PKH10_04135"
     CDS             3836..4039
                     /locus_tag="PKH10_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015218234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27350.1"
                     /translation="MANLEVDRAVFSVGDLADDTEEKRYWLSCTPAQRLEAIETNRRI
                     IYGYLDTPPRLQRFFEVAELKAG"
     gene            4017..4421
                     /locus_tag="PKH10_04140"
     CDS             4017..4421
                     /locus_tag="PKH10_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008310868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27351.1"
                     /translation="MLNSKQVEYLLVGGYAVGYYGYPRATADIDVWIAVSEQNAGRIV
                     SALREFGFAVPDLKPELFLQKGRVIRMGVPPIRIEVITSASGVDFAECYSRRLVVEMD
                     GIPVSLIELTDLKRNKKAAGRPKDLADLAQLA"
     gene            complement(4432..5742)
                     /locus_tag="PKH10_04145"
     CDS             complement(4432..5742)
                     /locus_tag="PKH10_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNT27352.1"
                     /translation="MNAVAALKELQAARIETLSGGAPAAFAYGADATFRAPDTLFVVL
                     PATEEELVAAVRILVEHRIPMTPRGSGTGTTGGALPEGSAVLFTGRLTACRIDARDLI
                     AEVEPGVLTGDLKKEAEKQGLLYPPDPASHEYSTVGGNIATNAGGPSSLKYGTTRDHL
                     RKVRIVDGTGKVRELGAAVHKFSTGYFLPGMICGSEGTLGVVTRAWMKLVPYPRFASF
                     IEVVAPTFEDLATLRSLAPAALEYLDARAGELVTGSRQSYLLLRLEAFTEAELAEQER
                     RIAGRLTSAGRFFRMGRDKKEIWAVRELLSPLSYQLGERKTAQDIVLPFSRIPEFLQW
                     AEQLRVRLPAGMELFVFGHLGDGNLHVNLLSSQDLAVEAKRIQREIVSQTRQLGGLIS
                     GEHGIGRERREALGLFIDEPTRELMRDIKKLFDPYDLMNPGKVL"
     gene            complement(5739..6581)
                     /gene="lgt"
                     /locus_tag="PKH10_04150"
     CDS             complement(5739..6581)
                     /gene="lgt"
                     /locus_tag="PKH10_04150"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNT27353.1"
                     /translation="MRPTLIDLGWFSLPSYYTMGMLGVIVGFFLLDREAKRQGLDRRV
                     AFNAGVYAAIFGYFGARIQHIIFDGFFEIYLQKPIAMLYFWKGGLAFYGAIVLGSAAI
                     VFYLTVVRRPVMRYLDLFTLPCALGLVLGRIGCTLNGCCFGKVSDGPLSLTFIVNGLS
                     AKKQFTDTMLASLRDMPYPVIATQPIEAVLGFSLFLGLFFARKRVTKPGVMFGSWLIG
                     YAIIRFIIEIFRDDERGLFFGGLLSTSQIIAVLTIVLGVLLVLLPAEDERRSSDVQPE
                     EISR"
     gene            complement(6578..>6899)
                     /locus_tag="PKH10_04155"
     CDS             complement(6578..>6899)
                     /locus_tag="PKH10_04155"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNT27354.1"
                     /translation="FSELYKLVVLRGFSTLVTLVVLALVFVRTPHKLYPVIYAFIIAG
                     AAGNLIDRFAYGFVIDFIFWHLGDLYHHPVFNIADAAIVVAIGLVILETFFLRERKNG
                     QVAA"
BASE COUNT         1333 a         2172 c         2045 g         1349 t
ORIGIN      
        1 cgtatcggcc tctcccttga gccgccgtat ctcgcgctgg acggcgttca gggcctgcac
       61 ggtcttcaga tccttgaggt tcgccgggaa cgagacctgc cgcaattcct cggtggtcac
      121 tccgatgaag gcgagacggc ttccttcgcg atcgaggatg tgcgacgcgg ggaacaccgg
      181 cttcatcccc ttgccgatga tattggccga cagatacttt atcttcgacg cggtacggtt
      241 ctcatcgaac tcggtcatcc cgtagtcgaa ctcgtggttg cccactgccg cgatctgata
      301 gccgatgtcg tgcatcgcct cggcggcgga gcgccccttg aatatcgacg agatcgccga
      361 accggtgtag ttgtcaccac ccgatacccg tatcacgatg caccccgcgc agtcgcgctc
      421 ttcctctcgc cacatctcgt cgaggatgac cgccttctct cgaccttcgg gctcccagat
      481 gtgaccgtgt tcgtcattgg tatagaggat gaggtaccgt tgcggtgcct cttcggtgac
      541 cagatcagcc agtttttcgc aggaggagaa caagagggcc agtatcgtga ggaccagtag
      601 cctgctcggt atcatcatcc acccgcgata agggttcaac cacgcggcga tattaacttt
      661 tcccgcgaca aagtaaattt ttttgtcgaa ggttgtctta ccgttcgttc tccgttagaa
      721 tgcggaccaa aggaggatcc tccgtgcaag gcacctcgac ggaacacatc gtcatcatcg
      781 ccggcatcgg cgccgtgcta ctgcgccgcc atgcccgcgt ccggaacatc gtcctacgcc
      841 tcaacgacga cggcgacccg gtggcgacgg tcccgcactg ggtcccttac ggagaggcga
      901 tagatttcgt catctcgcgc cgcgactgga tcacccgtca gcaacgcgaa cgggccgccg
      961 caccggccgc accgccacca ctcccctccg aaccgctctc catcacgcgg gaggaggctc
     1021 gcccccttct cgaacggcgc atcgccgaac tcgcccggcg gcatgacctc gtcgtgacac
     1081 gtgtcaccgt cagggctcag cgtaccgtct ggggttcatg cgcggtcgac ggcggcatca
     1141 acatcaactg gagaacggcg cgactccccg acgccctgcg cgactatgtg atcctgcacg
     1201 aactggccca cctgatctac cgcagccacg gcccccgcta ctggcgcacc ctcgatcggc
     1261 tcgtcggcgg cgacgcgcgg acgctcgacc gggaactccg gcgctggccg ctcgcggcgc
     1321 tctgacattc ttgcaatacc gcggtccgcc gctatgatgt gtacgacaaa aacggagaga
     1381 atggtatcgc cgcgatgacg ctcttgctcg aaggtcttct tctcgggctt tcgctcgggg
     1441 tcacctgcat cgccacctgc gccccggtct acgcccctat ccttatgcag accgaacggg
     1501 gcacgttggg cgcgttgtgg gccgtggtgc tcatgagcgc ggggcgtttc ctcagttacg
     1561 ccgcggtcgg cgtcgccgtc ggggcgatcg gggccgccgc cgccgggacg ctccccatcc
     1621 cgaccatcgc cgccgtttcg tatctgttca tcgctctttt tctcatatac acggcgctca
     1681 tccggggacg cgccgaaaag cacggcaact gcccggcggg gctcatgtcc cgttttgccg
     1741 ggaacccgtt cgtcgcgggg ctcgtgaccg gtttctcggt ctgtcctccc tttatcggcg
     1801 ccatcacgcg cggcttcgat accggcggcg cgctcggcgg gatgctcctg ttcatcggct
     1861 ttttcgtcgg caccacgctc tacttcatcc cgatggcgat cctgccgctc ctcgcgcacc
     1921 gacggctcat ccgcatcgcc gggatattct tctcgctctg cgccgcgttc tggttcaccc
     1981 tcctcggtct cgatcatttc ctcgacctgc ggatcaaggc cgagatgtat cttgaggatg
     2041 cctatctcgt tgactattcg caggcggaac tgacgctggt cgatgcgacg ggcgatcccg
     2101 ccctgaccga acgggcagcg cggttctttc cgcatgcccg ggcgatgacc gcctccgcgg
     2161 aacgatatgc tgaaacactc gctgcgctcg ccccggcgac caatgtcctc ctgttggtga
     2221 cgacaacgcc tgatgagagc gtgagaacgc tcgccatcga gaaaagactc aacatcgtct
     2281 gggccatcgc cccgcgcacc gacgccggga cggtcgaact acgcgatttc cttgccacct
     2341 acatcatcaa ggctcgccaa agcagagggt tcctgttccg cctgccgctc gacccccggt
     2401 gaccgtgcgg aatcgcactg agggaaatct tgattttcca aagtaaatcc ttcaccatgg
     2461 ggacgggttc ttcttttatg tgacaaggga ggtttcaggt catgaaaaag atggtgttcc
     2521 tgttctcgct tttcgcggcc atcgcgctca acgccgagat caaccgcccg gtgtacgaga
     2581 ccgggtatga aagttacgcg ccgctcggca atacgccggt gctgtcgcag ttcgtcacct
     2641 tcgaggacgc ctactggctc ccgtcggtcc tgcccgaact cgggaagggc tgggtctaca
     2701 tcaacaacaa cggccagtac ggcggcgtca tcttcccgct ccttgaacgg aaactgttca
     2761 tgcatgtctt tgtcgaatac gacccgaacg aagcgaacta cacgcgcaac agcccgcggc
     2821 aactggtcga gatcatgcgc ggggtcagtt tcgacaccct cggtaacgac gagtacatcg
     2881 caccgctctc caacatcttc ggcttcggtt tcgcggggga tgtcgggacc ttgtcctggg
     2941 gcatcaacct gaaactctac gccgaccagt actcccggaa aacgaccacg cccgcgacaa
     3001 cggccaccct ttcgaattcg accttccgtt tggaattcac cccgtcgatc tccatgaagc
     3061 gcgatgaatg gcgcatggat gccggattca acctgctcgg acagtggata tcgaacgaga
     3121 catccaatca gaccttcgtg tacccgtacg attacgccgg caacgccgag ttcgcgatct
     3181 acggccggat gatgtacgat atcacgaaat acacgacgct gatgggcaac ctctccttcg
     3241 ggtacgccgg cgcgtcggac aaactggaca atgaccatgt gtcgctcgac aacacctatc
     3301 tcgtcctgaa gggcggcgcg ctgataaagc cggtcgaacg gctgacgctc ggcagcaact
     3361 tctctttcta ccatgcgtgg caggggctca accgcacgac ctcgcccggc ggcgtcacga
     3421 ccaagaccga ggattcgggc aacctgctgg tgttcaactt cacccagacg gccgatttca
     3481 agccgtgcga ctggttcacg ctcaaggcgg gcatcggcaa gaatatcttc gtcaatgtca
     3541 gcaccgacaa ggtcatcgtc ccggccggga ccagcaccga cgacgactac gacaacaatt
     3601 tcgatatcgg caaattcatc ggcttcgcga tagactacaa ggaattcaac ttcaccgcgg
     3661 tcatgaactt cgatgtcttc accgagggcc cgtacctgct caccggccgc gcgtgggcga
     3721 atccgatggc cttcgtcgct tccctgaatt accagtggta gtaccccttt tatcttttct
     3781 tgactcgcct agcgtctgaa tctatacttt ctctggtaca cggggcgtat tgtacatggc
     3841 aaatcttgaa gtagaccgag cggtcttcag tgtcggcgat cttgctgacg acaccgaaga
     3901 aaaacgctac tggctttcct gcacacccgc ccaacggctt gaggcgatag aaaccaatcg
     3961 acggataatc tatggttatc tcgacactcc cccccgactt caaagatttt ttgaggttgc
     4021 tgaactcaaa gcaggttgag tatctgctgg tcggcgggta tgccgtcggc tactacggat
     4081 acccccgcgc caccgccgat atcgatgtct ggatagctgt ttcggaacag aatgcggggc
     4141 gcatcgtctc ggcgctccgt gaattcggat ttgcggtgcc cgatctgaaa ccggagttgt
     4201 tccttcagaa gggccgggtc attcgcatgg gcgtcccgcc gatcaggata gaggttatca
     4261 cttcagcctc cggcgtcgat ttcgccgaat gttattcacg gcggcttgtt gtggagatgg
     4321 atggcattcc ggtatcgctt atcgaactga ccgacctgaa aaggaacaag aaggcggccg
     4381 gacggcctaa ggaccttgcc gatctcgctc aactcgcgtg atcttgttca cctagagcac
     4441 tttccccgga ttcatcaggt catacggatc gaagagtttc ttgatgtcgc gcatcagttc
     4501 gcgtgtcggc tcatcgatga acagaccgag tgcctcgcgc cgttcgcgac cgatgccgtg
     4561 ttctcccgat ataaggccac ccagttggcg ggtctggctg acgatctctc gctggatgcg
     4621 tttcgcctcg accgcgagat cctgtgagga aagcagattg acatgaagat tgccgtcgcc
     4681 gagatggccg aacacgaaca gttccatacc ggcgggaagc cgcacgcgca actgttcggc
     4741 ccactggagg aattccggta tccgcgagaa gggcagtacg atatcctgcg ccgtcttgcg
     4801 ctcgccgagt tgataggaaa gcggcgacag cagttcgcgt accgcccaga tctccttctt
     4861 gtcgcgtccc atgcggaaga accgccctgc ggaggtcaaa cgaccggcga tgcggcgctc
     4921 ctgttcggcg agttccgcct cggtgaaggc ctcgagccgg aggagcagat aactctgccg
     4981 cgagccggtc accagttcgc ccgcccgtgc atcgaggtat tcgagcgccg ccggggcgag
     5041 cgaacgaagc gtcgcgagat cctcgaaggt cggggcgacc acctcgatga aactggcgaa
     5101 gcgtgggtaa ggtaccagtt tcatccatgc gcgggtcacc acgccgaggg ttccctcgga
     5161 gccacagatc atgccgggga gaaagtagcc ggtcgagaat ttatggaccg ccgcccccag
     5221 ttcgcgcact ttcccggtgc cgtcgacgat ccgcaccttg cgcaggtggt cacgcgtcgt
     5281 gccgtatttg agcgacgagg ggccgccggc gttggtggcg atgttgccgc cgacggtcga
     5341 atactcatga ctcgccgggt caggaggata caagagtccc tgtttctcgg cctccttctt
     5401 gaggtcgccg gtcagcaccc ccggctcgac ctcggcgatg aggtctctcg cgtcgatgcg
     5461 gcaggcggtg agccgtccgg tgaacagcac ggccgatccc tcgggcaggg cgccgccggt
     5521 ggtgccggtg ccgctgccgc gtggcgtcat cgggatgcgg tgctccacga gtatccggac
     5581 cgcggcgacg agttcctcct cggtcgcggg taggacgacg aagagggtat cgggggcgcg
     5641 gaaggtggcg tcggcgccgt aggcgaaagc ggcgggggcg cctcccgaca gcgtttcaat
     5701 acgggctgct tgaagttctt tgagtgccgc gacggcgttc accgggatat ctcctcgggc
     5761 tggacgtcgg aagaccgccg ctcatcctcg gcggggagca ggacgagcag tacaccgagc
     5821 acgatggtga ggaccgcgat gatctgcgag gtcgagagca ggccgccgaa gaacaggccc
     5881 cgctcgtcgt cgcggaatat ctcgatgata aaacggatga tcgcgtaccc gataagccac
     5941 gaaccgaaca tgacccccgg ctttgtcacc cgcttccggg cgaagaacag cccgaggaac
     6001 agggaaaagc ccagcaccgc ctcgatcggc tgggtcgcga tgaccggata gggcatgtcg
     6061 cggagcgatg cgagcatcgt atcggtgaac tgtttctttg ccgacaggcc gttcacgatg
     6121 aaggtcaggg agagcggccc gtcactcacc ttaccgaaac aacagccgtt cagcgtacag
     6181 ccgatacggc cgagcacgag ccctaaggcg cagggcaggg tgaacagatc gaggtagcgc
     6241 atcaccggcc gcctcaccac cgtcaggtag aacacgatgg cggccgatcc gaggacgatg
     6301 gcgccgtaaa aggcgagccc ccccttccag aagtagagca tcgcgatcgg cttctgcagg
     6361 tatatctcga agaaaccgtc gaagatgatg tgctggatgc gcgccccgaa atagccgaat
     6421 atcgcggcgt aaacgcccgc gttgaaggcg acacgccggt cgagtccctg ccgtttcgcc
     6481 tcgcggtcga gcaggaagaa gccgacaatg acgccgagca tgcccatcgt gtagtaactc
     6541 ggcagggaga accagccgag atcgatgagc gtcggtctca tgcggcgacc tgcccgttct
     6601 tccgttcgcg gaggaagaag gtttcgagta tcacgagccc gatagcgaca acgatggcgg
     6661 cgtcggcgat gttgaacacc ggatgatggt acaggtcgcc cagatgccag aagatgaaat
     6721 cgatcacgaa cccgtatgcg aaccggtcga tgaggttccc cgccgcgccg gcgatgatga
     6781 aggcgtagat gaccgggtag agtttgtgcg gcgtccggac gaagacgagc gcgagcacaa
     6841 cgagcgtcac aagcgtcgag aagccgcgca ggaccaccag tttgtacagt tccgagaag
//
LOCUS       DAOQUE010000093         6893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12115_length_6893_cov_5.407575, whole genome shotgun sequence.
ACCESSION   DAOQUE010000093 DAOQUE010000000
VERSION     DAOQUE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12115_length_6893_cov_5.407575"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="PKH10_04160"
     CDS             complement(<1..1302)
                     /locus_tag="PKH10_04160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT27355.1"
                     /translation="MRRTAIILFLAALFLRALCIPFMHITYDEAAYSWTARDLALNGG
                     WLDLYGRDDLFFFPPLFNYIAALFIKLGIDRLYAVRLVTTLFSSGIPPLMYLLALRSG
                     LSERVGLFAAVLWIILPWGWYLSVAGMVETPWLCCLLLAVYFLQNAAERGSRRDALLS
                     ALAFAAALWMKETALGALPLFLFALRKDRSLLARWAGAALLLSLPLFLQSILPHRYDL
                     FFEITTPLLLWRSFEIDALLRNIGTLYGAGSFPWAGWDLSLSFLLLTVAILSIIAVPR
                     AEWRGRFLFRFAAGFFLIYLPFFALFPKKFHYYLLPVILLSCFFIARYLATRPRLAVA
                     YGLVAVLLAVPAFLKLADRSEEEWYREAFRVAMQEKTDARIGLTLPRKAEYIAERAGM
                     RPDIVPVEWVECAGNGDPCIFRNDFLLSDPMFLMMLYCRQWP"
     gene            complement(1309..2058)
                     /locus_tag="PKH10_04165"
     CDS             complement(1309..2058)
                     /locus_tag="PKH10_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNT27356.1"
                     /translation="MTLDALYDILLAAQFISAAFVFVALYFITVPYGRHTHTGWGPLV
                     PSRPGWVVMELPAVVVIAALFATGDITLVGTVFLCIWQLHYLQRTFLFPLLMRGGRGT
                     SVVIVLFSVMFNVMNGWVNGWYLYRRAAYDISWLWDPRFIVGLAVFIAGFVINLHSDH
                     VLRKLRRPGETGYKIPMSGLHRLVASPNYFGELLEWTGWAILTWSYAGLAFAVFTAAN
                     LVPRAHAHRKWYRATFPDYPVERKRILPFIY"
     gene            complement(2106..6596)
                     /locus_tag="PKH10_04170"
     CDS             complement(2106..6596)
                     /locus_tag="PKH10_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNT27357.1"
                     /translation="MAVDLVLLEIYQLSEMVSMNILNPLTQVGTLNISVANANEAMWN
                     SIISGASPLSAQQRITTYYFRVEHNIFIPLEDVSIDEPVRVDSILGKNNWIQGGFFND
                     YLENAAHAYYPIIGCTQLSAAQIMKKWNYPSTMNVDLTYIDDENVGSGEAIYVNDGRF
                     VCPDIGKKAPYDNDPTIDWGSILPFYDRMDHWASVCYSPTCNAAKRYLYNVAILFCPD
                     FGSEATSNPIARSLSVMPQFFRYSKDIASMPADDPLKISNIKRQMILGVPVQGETDGD
                     DGDSGHAFVVDGFDDTDSDNIKIHVNLGWHWPKDTRFWVNANDALGHDSGQGPLQSII
                     YNIFPETYCGNDQYAEECNDKDGVPFYKDKCPRVYNPENSNIYYCKDKDRDGFCEQPD
                     VLVQSDCSWSCINWVKANYYMEIDYSSVNYTSAPFKKGKTYKTNNQIDVEVREIEDWP
                     NTKKAKIYVSSKDFLLGFQLTGIYSSEGNYMGHIDAVERICNPEEGVEISGERFLFAK
                     ETYDDGQIKTSRNRPSDNCPSVYNPDQHINSLVSGMVGTACDIDKNGIDDTTQFDLFY
                     VEGQNALSYSESTSSTSSSCGSFSFPGTCFNVTAEEGAVSEVTGTNKIMDKNHPASPE
                     MYGAVYYCLCGTEKNEKWRECRHNSVEGKCGINMASPAGSPFDKEKNLYKWRPASTDK
                     SWDLFRGTKNNRLITKDITWSQHPTGAFTKGYSEKWYWQTDMEREGLNASQWYREFPY
                     TDPQPPYNTYLRSSQYMDDYYAIFPTVMLSFSPVTTSQTDKYVLDANGRPVSETQTAW
                     ANYNDTNFPDVNAGYFKNSRGNFSTNRFYPAIAHTVKEANLLHSKISFLRFIPFDTPF
                     KLHDTLLEQLMEGHWLDDPSGPVQDIPIQDEPWGRPGLVFDTQFDLAGDNILLKKLRL
                     PNGWMSFVLTQGGGFSSYVKNADGTYALKTIDSTGNMESAFAISGGSDFYFGAMSKKG
                     ENYYLAGNLQVTGFVTTAGYAYTLGSKFGKLSPTDTSGYAFSQLADLPSPSTSLKLFS
                     HNDVLYAIQSVDTENPKAVKIAKYNEGENLWEIVARIETTESVVVKSVIPQTTTVYFL
                     VNGTTSKVYEYNPALTENNVIERATLLASNVRMYDNSGLFVALNLETLKAPPVMAYNI
                     SAEGLVEEREVIIEGYNFDISSTVTNSYCLHEVAGTLKGGVEGNLACQPFTHPWYKSF
                     SIGTTVYSVAGKGDRLYVGTSNAIKVYDISDPNALTLKSTFTTNKIVYDLEVVDGDIM
                     YAATSKGLYKLSTANPDTLSIIGSFYSTGSYNYQYRIQLYNDKLYVGDDNGINIRDKE
                     TFTRLAYVNIGSTLDFAIANGELAMYWDDFWVSGLDIRDADTLTRKAWENPYCSTGEL
                     TTDHGAFYLSCDGYEYRFAGRPDTYIDFWELSGDIREMAENHLYNGWVYIPDGNKVKV
                     STNNEVSSYCGNGIVEPGEVCDGNSVYCEDLDPNEWNSGTATCKSDCSGYNTGDCYWS
                     GC"
BASE COUNT         1688 a         1718 c         1839 g         1648 t
ORIGIN      
        1 cggccactgg cgacagtaga gcatcatcag gaacatcggg tcactgagca ggaagtcgtt
       61 gcggaatatg cagggatcgc cgttcccggc gcattcgacc cattcgaccg gcacgatgtc
      121 cggtcgcatc cccgcccgct cggcgatata ctccgccttc cgcggcaggg tgagcccgat
      181 gcgggcatcc gtcttctcct gcatcgccac gcggaacgcc tcacggtacc actcctcctc
      241 ggaacgatcg gccagtttca gaaaggcggg cacggcaagc agtaccgcga caagaccgta
      301 cgccacggcg agccgcggcc gcgtcgccag ataccgcgcg atgaaaaagc aactcagcag
      361 gatcaccggc agaagatagt aatggaactt tttggggaac agcgcgaaga acggaagata
      421 gatgaggaag aacccggcgg cgaaccggaa caggaaacgc ccccgccatt cggcgcgcgg
      481 cacggcaatg atcgaaagta tcgcgaccgt caggagcagg aacgacagac tcaggtccca
      541 gcccgcccac gggaaggagc cggcgccgta gagggtcccg atgttcctca aaagagcatc
      601 tatctcgaac gaccgccaca aaaggagcgg cgtggtgatc tcgaagaaca gatcgtaccg
      661 gtgcggcagg atcgactgca ggaagagcgg cagagaaagc agaagcgccg cgcccgccca
      721 acgcgccaga agcgaccggt ccttccgtag cgcgaagagg aagagcggca gggcgccgag
      781 cgccgtttcc ttcatccaga gcgccgcggc gaaggcgagc gccgagagga gcgcgtcgcg
      841 ccgggatccc cgttcggcgg cgttctgcag gaaatagacc gcgaggagca gacagcacag
      901 ccacggcgtt tcgaccatgc cggcgaccga caggtaccac ccccacggca ggatgatcca
      961 gagcacggcg gcgaagagcc ccacccgttc ggagagcccc gatctgagcg caaggagata
     1021 catgagcggc ggtatgccgg aggagaaaag cgtcgtcacg agccgcaccg catagagccg
     1081 gtcgatgccg agtttgatga aaagggcggc gatatagttg aacagcggcg ggaagaagaa
     1141 caggtcgtcg cggccgtaca ggtcgagcca gccgccgttc agcgcaagat cgcgggcggt
     1201 ccaactgtag gccgcctcgt cgtaggtgat gtgcatgaac gggatgcaga gcgcccgcag
     1261 gaaaagcgcc gcgagaaaaa gtatgatcgc cgtccgtctc atcgcaccct agtagatgaa
     1321 gggaagtatc cgtttccgtt cgaccgggta gtcggggaag gtggcgcggt accatttccg
     1381 gtgggcgtgg gcccgcggca cgaggttcgc ggcggtgaac acggcgaagg cgagccccgc
     1441 gtaggaccag gtgagtatcg cccagccggt ccattcgagc agttcaccga agtagttggg
     1501 gcttgcgacc agccggtgca gtccgctcat cggtatctta tagccggtct cgcccggcct
     1561 gcggagcttc cgcaggacat ggtcgctgtg caggttgatg acgaacccgg caatgaagac
     1621 cgcgagcccg acgataaagc gcgggtccca cagccacgag atatcgtagg ccgcgcgacg
     1681 gtacagatac cagccgttca cccagccgtt catcacattg aacatcaccg agaacaggac
     1741 gatgaccacc gaggtgccgc ggccgccgcg catgagaagc gggaaaagaa aggtgcgttg
     1801 gagatagtgg agttgccaga tgcagaggaa cacggtgccg accagcgtga tatcgccggt
     1861 cgcgaacagc gccgcgatga ccaccaccgc cggcagttcc atgacgaccc agccgggccg
     1921 cgacgggacg agcggccccc agccggtgtg ggtgtggcgg ccatacggga cggtgatgaa
     1981 gtagagggcg acaaagacaa aggcggccga gatgaactgg gccgcgagca ggatgtcgta
     2041 cagggcgtcg agcgtcatga tgcctccgtg agcggtggtc ttggacccaa gcggcgccga
     2101 tcccgtcagc accctgacca gtagcagtca ccggtattgt agccggaaca atcgctcttg
     2161 caagtcgccg taccgctgtt ccactcgttc gggtcgagat cctcgcaata gacgctgttc
     2221 ccgtcgcata cctcgcccgg ctccacgatg ccgttcccgc agtaactcga cacctcgttg
     2281 ttcgtcgaga ccttcacctt gttaccgtca gggatataga cccagccatt gtacaggtgg
     2341 ttctccgcca tctcccgtat gtcgccgctc agttcccaga agtcgatgta ggtgtcgggc
     2401 cgcccggcga aacggtactc atacccgtcg caggagaggt agaacgctcc gtggtcggtc
     2461 gtcagttctc cggtcgagca gtacggattt tcccatgcct tccgggtgag ggtatcggca
     2521 tcgcggatat cgaggcccga tacccagaag tcgtcccagt acatggcgag ttcaccattg
     2581 gcgatggcga agtcgagggt gctgccgatg ttcacatagg cgagacgggt gaaggtctcc
     2641 ttgtcgcgga tgttgatgcc gttgtcgtcg ccgacataga gtttgtcgtt gtagagttgg
     2701 atgcggtatt ggtaattata ggaaccggtg ctgtagaagg aaccgatgat cgaaagcgtg
     2761 tcgggattgg ccgtgctgag tttgtagagg cctttgctcg tggcggcgta catgatgtcg
     2821 ccgtcaacga cctcaaggtc gtacacgatc ttgttggtgg tgaaggtgct cttgagtgtg
     2881 agggcattcg gatcggagat gtcgtagacc ttgatggcgt tactggtgcc gacatagagc
     2941 cggtcgcctt tcccggcgac gctgtagacg gtcgtgccga tggagaagga tttgtaccac
     3001 gggtgggtga agggttggca agcaaggttc ccctcaactc ctccttttaa tgttccggca
     3061 acttcatgca agcaataaga gttggtcacc gtactcgata tgtcgaaatt gtatccctct
     3121 atgataactt cgcgttcttc gacaaggcct tccgcgctaa tgttgtaggc catgacggga
     3181 ggtgctttca atgtctcgag atttaatgct acaaacaggc cggaattgtc atacatacgg
     3241 acattgcttg ccaatagagt ggctctctca atgacattgt tttccgtcaa tgccgggttg
     3301 tattcgtaga ctttgctcgt agtcccgttg acaagaaaat agactgtggt cgtttgaggt
     3361 atcacagatt tcaccacgac gctttccgtc gtttcaatac gagcaacgat ttcccatagg
     3421 ttttcgcctt cgttatattt cgcgattttc accgccttgg ggttttccgt atcgacggat
     3481 tgaatagcat aaagaacatc attatgtgaa aagagtttta gagatgttga cggagacggg
     3541 agatccgcta attgcgagaa tgcgtacccc gaagtatccg ttggggacaa tttcccgaac
     3601 ttcgatccca gcgtgtatgc atatcctgcg gtagtcacaa atccggtgac ttggaggttc
     3661 ccggcaagat agtagttttc ccccttcttt gacattgcgc cgaaataaaa gtccgagccg
     3721 ccggaaatcg caaaggcgct ttccatattg cccgttgaat ctatggtctt gagagcgtaa
     3781 gtaccgtcgg catttttgac ataggacgag aatccgcctc cttgcgtcaa aacaaaagac
     3841 atccatccgt tcggcaaacg gagttttttg agaagaatat tgtcgcccgc taagtcaaat
     3901 tgtgtgtcaa aaacgagacc ggggcgtccc catggctcgt cttgaatagg aatatcctga
     3961 acgggaccgg agggatcgtc caaccagtga ccttccatta gttgttccag taaggtgtcg
     4021 tgcagtttaa aaggtgtatc gaaggggata aatcgcagaa aagagatttt cgagtgaagg
     4081 aggttggcct cttttaccgt gtgggcaatg gcagggtaaa atctattagt tgagaagttc
     4141 ccgcgactgt tcttgaaata ccccgcgttc acatcgggga aattggtgtc attgtaattt
     4201 gcccatgccg tttgtgtttc cgaaacggga cggccatttg cgtcaagcac atatttgtcg
     4261 gtctgactcg tcgtaacagg agaaaaagac aacatcaccg taggaaatat cgcgtagtaa
     4321 tcatccatgt attgtgaaga acgaagatag gtgttatagg gtggttgagg atcggtgtaa
     4381 ggaaattctc tataccactg ggaggcgtta agtccttctc tttccatatc ggtctgccag
     4441 taccacttct cactgtatcc tttggtaaaa gcccctgttg ggtgttgtga ccaagtgata
     4501 tccttcgtga taagacgatt gttttttgtg cctctaaaaa gatcccaact tttgtccgtc
     4561 gatgcagggc gccacttgta aagatttttt tctttatcaa aaggagatcc tgcgggagaa
     4621 gccatattga tgccgcattt tccctctaca ctattgtgtc ggcactctct ccatttttca
     4681 ttcttttctg tgccacacaa acaataataa acggcaccgt acatttcagg tgaagcgggg
     4741 tggttcttat ccattatttt gtttgttccc gtgacctcgg aaacagcgcc ttcttcggct
     4801 gtgacattaa aacaggttcc cggaaaacta aatgagccac atgaagagga agttgacgaa
     4861 gtgctctctg agtaagagag agcgttttgt ccttcgacat agaacaagtc gaattgtgtc
     4921 gtatcgtcga tcccgttctt gtctatatcg caagccgttc ccaccattcc cgagacaaga
     4981 gagttaatat gctgatcggg attgtatacg gatggacaat tgtcagaagg tctattccgc
     5041 gatgttttga tttgcccatc gtcgtaggtt tcttttgcga acagaaatcg ttctcctgat
     5101 atctcaacac cttcttccgg attacagatt ctttcaactg cgtcaatatg tcccatataa
     5161 tttccttccg aagaataaat acccgtaagt tggaatccta aaaggaaatc cttagaagag
     5221 acatatatct ttgctttttt tgtgttcggc caatcttcta tttccctcac ttcaacatct
     5281 atctggttgt ttgttttata tgttttaccc tttttgaaag gggcagatgt atagttcacc
     5341 gatgaatagt caatttccat atagtaattc gcttttaccc agtttataca agaccaagaa
     5401 cagtcgcttt gtaccaaaac atccggttgt tcgcagaaac catctctatc tttgtcttta
     5461 cagtagtata tgttgctgtt ttctggattg tagacccggg ggcatttgtc tttgtagaaa
     5521 ggcactccgt ctttgtcgtt acattcttca gcatattgat cgttgccaca ataagtttct
     5581 ggaaaaatgt tatagattat agattgtaat ggcccctgtc cggaatcatg ccccagcgca
     5641 tcattggcat taacccaaaa tcgagtatct ttaggccagt gccagccaag attaacatgt
     5701 attttaatat tatcagaatc cgtgtcatca aatccatcca caacaaaagc atgaccgcta
     5761 tcaccatcat ccccatctgt ttctccttgc accggtactc cgagaatcat ttggcgctta
     5821 atgtttgata tttttagagg atcatctgcc ggcatcgacg caatgtcttt agaatatcta
     5881 aaaaattgcg gcataactga caatgatcta gcgatggggt tgctagtcgc ttcgctaccg
     5941 aaatcgggac aaaataatat tgcgacattg taaaggtatc ttttcgcggc attacaggta
     6001 ggggagtagc acacagaagc ccaatgatcc attctatcgt aaaaaggaag aatgctaccc
     6061 cagtctatgg tcggatcatt atcataggga gccttcttgc ctatatccgg acaaacgaaa
     6121 cgaccatcgt tgacataaat tgcctcaccg ctaccaacat tttcatcgtc aatgtatgtc
     6181 aagtcaacat tcatagttga agggtaattc cattttttca tgatctgtgc ggcagaaagc
     6241 tgggtacatc ctatgatagg gtaatatgcg tgcgcagcat tttctaagta atcattaaag
     6301 aagccacctt gaatccaatt gtttttaccg agaatagaat ctacacgaac gggttcatcg
     6361 atgctcacat cctctagtgg aatgaaaatg ttgtgttcca cccggaagta ataagttgtt
     6421 attctttgtt gggcggatag ggggcttgcc ccagaaataa tactattcca cattgcctca
     6481 ttggcatttg ccaccgatat gtttaatgtt cccacctgtg ttaacgggtt aagtatattc
     6541 atggaaacca tttctgacaa ctggtaaatc tctaaaagaa ccaggtctac agccaaactc
     6601 cgaatgatag attttataaa aggatgttcg gaagcgttag cctgggagcc atcgggcaaa
     6661 gacatcggct caaagaaaga tccagcaaag gacaacaact tgaaatcgcc agattctctt
     6721 gtcttgggta aaatgaaaaa tccttcggga cttaatggta tcacccatcc taataccaca
     6781 ccgttatact ctagggatga aatatcgtcc tgcgagatag aatattcgac atcattgtct
     6841 gggtccttat aagaattaat tagattattc cagtaaggtg cgcttgtcag aat
//
LOCUS       DAOQUE010000094         6880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12146_length_6880_cov_3.794579, whole genome shotgun sequence.
ACCESSION   DAOQUE010000094 DAOQUE010000000
VERSION     DAOQUE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12146_length_6880_cov_3.794579"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /gene="gatB"
                     /locus_tag="PKH10_04175"
     CDS             complement(<1..1406)
                     /gene="gatB"
                     /locus_tag="PKH10_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNT27358.1"
                     /translation="MTRYEAVIGLEIHVQIQTATKAFCRCEGDYNAPPNRNICPVCTG
                     APGALPVLSDEFVARAVRLCLALGARVNLLSTFDRKNYFYPDMPKNYQITQFFTPIGS
                     EGRLLVTGADGVERAIGIERIHMEEDTARSAHLGERTLLNFNRAGLPLIEVVSHPDLK
                     SGEEAAQYFKKIYDIAVKYLGICRGNMEEGNLRCDANISIRPAGSTALNTKTEVKNVN
                     SFAFIAKAIDHEIERQIAIVEAGGEVESETRLWNPKLKRTETMRKKSGRNDYRYFNEP
                     DLGPLRITAEFVEELRTTLPELPDDKRRRLREAYGINNEQLDLFIGYPAVCEYFERAS
                     SSATASFSTIAAWTSGELLRVEDKENFGAVQTPPEHIASLARLIDTKKVNGPQAKEIF
                     AIMRETGSDPEEIVKNDPRFTAIGDDETAAIAAAVVADHPAETEKYLGGRENLLGFFV
                     GQAMKRAKGKADPAKMSE"
     gene            complement(1393..2754)
                     /gene="gatA"
                     /locus_tag="PKH10_04180"
     CDS             complement(1393..2754)
                     /gene="gatA"
                     /locus_tag="PKH10_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNT27359.1"
                     /translation="METLGVKDIYGNIAAHNGALNAYLQVVPEERLKPGAGPLSGVCL
                     GVKDNIAVAGLQLTCGSKLLETYVAPYTATVVERLVAAGATVTGKLNLDEFAMGSATT
                     YSAYGDTKNPWDEERTPGGSSGGSAAAVAAKMCDIALGSDTGGSVRQPASYCGIYGYK
                     PTYGTLSRYGLTAFASSLDQIGILARRPGHIRELMQVAAGRDGRDATSIEAPSFDAQA
                     FDPAKMRIGVIAEYEKNMGPAVRPAYDRCIETLKKAGATIVPLSFPEYDTVISVYQVI
                     ATAEASANLARFDGIRYTARAEGCSFRETTAASRGRWFGAEVKRRILLGTWVLSRAYY
                     DSYYLRAAKVRTVILNRWKEFFGKVDMVLTPATAGTAFKRAEQPDTFALYLKDLFTIP
                     ANLTGMPAIAFPAGTHDRLPVGLQLTADLSRDGLLLDAAAWLEKNVMGFATIERGCRH
                     DAI"
     gene            complement(2754..3077)
                     /locus_tag="PKH10_04185"
     CDS             complement(2754..3077)
                     /locus_tag="PKH10_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27360.1"
                     /translation="MTKPSDDIRAVYELAALTGEKDELTAMEGHFERMFKHLADLSRV
                     PVERVEPYFTLPLRELPLAKDAPAPSGVEAAIRGTFTQEAEGFLVVQRVVNRGGDACV
                     EGEEE"
     gene            3246..4289
                     /locus_tag="PKH10_04190"
     CDS             3246..4289
                     /locus_tag="PKH10_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT27361.1"
                     /translation="MEAYRIPEPVTFENYPDLLAAALRAIPAGTEVVLDLDGVRLDTA
                     TKAFVRRLIAVRGDAVRVPEKYCEEILYPRPRAESQKKGSFRWARVLDRVGDAIFSLI
                     DRMVFFGAIFVDMLYYAAQSLWDRRHLLRGDTIRNAFHIGYKAVPIVVLLSFLIGLTI
                     SIQAAIQMAKLGGQSYIATLIGIAMFTELGPLVTAIIIAGRTGSAIAAEIGTMVVMEE
                     VDALRTMGIRPLKFMLVPKFWAFTFSMPILTLVACFAGILGGLIVAQVYQVPPSAFVA
                     EIVKVMRMKDIVWGVIKSLTFAWVILAIACYQGLHVRGGADAVGRATTDSVVISIFMV
                     VVIDAIYSLILYT"
     gene            4299..5258
                     /locus_tag="PKH10_04195"
     CDS             4299..5258
                     /locus_tag="PKH10_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNT27362.1"
                     /translation="MTLFLLVGGFLLLTIGASILVDGAASVAKRYGISNLVIGLTVVA
                     FGTSSPEMVVNVVSALRGTTDIAIGNVLGSNIFNILAILGLSALIRRLPVQESTIRGE
                     IPLSLVAALLLFPLGAPYLSLPLDGSDPSALGRIDGIILLLFFAYFLRYTFKNARTGT
                     ESDGSVKLFSLPVSVAMIVAGFAMLVFGGKFLVDGAVIVARHFGIAERVIGLTIVAAG
                     TSLPELATSMVAAYKKNADIAVGNIVGSNIFNIFLILGISAVIRPLPFSTGTTVDLSV
                     TALCSMLLFVLVYRRTDRRLGRRDGALLVAFYVAYVSYLTVGT"
     gene            5269..5991
                     /locus_tag="PKH10_04200"
     CDS             5269..5991
                     /locus_tag="PKH10_04200"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNT27363.1"
                     /translation="MVTAIYIAGRGRVGAALVRHFGRQAVPCRVLKRGWEKRPPRRGV
                     LFLAVPDAAVERICREVFPTTPKGLTLVHFAGGLPISRERTHLLHPFASMGPESEFGR
                     LWCLWHGPADGGFERFLNTHHFHVIRRDPIPGPGYHVAAVLAGNFPQYLFLAARDLLR
                     REGFSDDDSKRLLVQLVETSVLGVREGGVGALTGPAARGDRAVMEREIVFLGKTAPRL
                     AVLYRALSELIAQAVGHGKILD"
     gene            5972..6871
                     /locus_tag="PKH10_04205"
     CDS             5972..6871
                     /locus_tag="PKH10_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HNT27364.1"
                     /translation="MAKFSTDPILDRFAEQVYARLVRDLCTPLPESFREIPYGVSFKV
                     ILPGEKEFPVALYHSEKKGFSVVTQSERVRRLILGLLAPEALAGCDEAGKGDIFGPLV
                     TACFYLCDGSRGALELGVRDSKRMGDDEVLAAADELDRRSRRFFEVVRIMPKRYNELY
                     ADFKKKGRTLNHLLAWAHGKALSTLAGRHPDMRQVLVDQFSDAPALVATIRAAAPDAE
                     VEFRPRAEAHPAVALASVVARADYLRSLKKLSDETLKGEFALRSGSGADADARIAAIR
                     ARHGDAVFAGLAKLHFANFSRER"
BASE COUNT         1097 a         2208 c         2168 g         1407 t
ORIGIN      
        1 aactcggaca ttttggccgg atcggccttt ccttttgccc gtttcatcgc ctgcccgacg
       61 aagaatccga gcaggttctc cctgccgccg aggtattttt cggtctccgc cggatggtcg
      121 gctacgaccg ccgccgcgat ggcggccgtc tcatcatccc cgatagcggt aaagcggggg
      181 tcgttcttta ctatctcttc gggatcactg ccggtttctc tcataatggc gaagatctct
      241 ttcgcctgcg gcccgttcac ctttttcgta tcgatcagcc gggcgagcga cgcgatgtgt
      301 tcgggcggcg tctgtaccgc gccgaagttc tccttatcct cgacccgcaa cagttcgccg
      361 ctcgtccatg cggcaatggt ggagaaactg gccgtcgccg atgacgacgc ccgttcgaag
      421 tactcgcaga ccgccggata gccgataaag aggtccagtt gttcgttgtt gatcccgtag
      481 gcctctctca accgccgccg cttgtcgtcg ggcagttcgg ggagcgtcgt ccgcagctcc
      541 tcgacgaatt cggcggtgat gcgcaatggt ccgaggtcgg gctcgttgaa gtagcggtaa
      601 tcgttgcggc ccgatttctt gcgcatcgtc tcggtccgct tgagtttcgg gttccagagc
      661 cgcgtctcgc tctcgacctc cccgcccgct tcgacgatgg cgatctgccg ctctatctcg
      721 tggtctatcg ccttggcaat gaaggcgaac gagttgacgt tcttcacctc ggtcttggtg
      781 ttgagcgccg tgctgccggc cggccgtatc gatatgttcg cgtcgcagcg gaggttcccc
      841 tcctccatgt tgccgcggca gatgccgagg tacttgacgg cgatgtcgta gatcttcttg
      901 aagtactgcg ccgcctcctc gccgcttttg agatcgggat gcgacaccac ctcgatgagt
      961 ggcagtccgg cgcggttgaa gttaaggagc gtccgttcgc ccagatgggc cgatctggcg
     1021 gtgtcctcct ccatatggat ccgttcgatg ccgatggccc gttcgacgcc gtcagcgccg
     1081 gtcaccagca gccgcccctc cgaaccgatg ggggtgaaga actgcgttat ctggtagttc
     1141 ttcggcatat cggggtagaa gtagttcttg cggtcgaagg tcgaaagaag gttcacccgc
     1201 gccccgagcg cgaggcagag ccggaccgcc cgcgcgacga attcatccga cagcaccggc
     1261 aacgcccccg gcgccccggt gcagacgggg cagatgttgc ggttgggcgg cgcattgtag
     1321 tcgccctcgc accggcagaa ggccttggtc gccgtctgta tctggacatg tatctcgagc
     1381 ccgatgaccg cctcatatcg cgtcatgacg gcacccccgc tcgatggtcg caaagcccat
     1441 aacgttcttt tcgagccatg cggccgcgtc gagaagcagc ccgtcgcgtg aaaggtcggc
     1501 ggtcagttgc aggccgaccg gcaggcggtc gtgggtcccg gccgggaagg ctatcgccgg
     1561 catgccggtg agattggccg gtatggtgaa gagatccttg aggtagagcg cgaaggtgtc
     1621 gggctgttcg gctcgcttaa aggcggtccc cgccgtcgcc ggggtgagca ccatatcgac
     1681 cttcccgaag aactccttcc accggttcag gatcacggtg cgaaccttcg cggcgcgcag
     1741 atagtaggaa tcgtagtagg cgcgcgacag cacccaggtc ccgagcagta tccgccgctt
     1801 cacctcggcg ccgaaccatc tgccgcggct cgcggcggtc gtttcgcgga atgagcaccc
     1861 ttcggcgcgc gccgtgtagc ggataccgtc gaaccgcgcg agattcgcgc tcgcctcggc
     1921 ggtcgcgatg acctgataga ccgatatcac cgtgtcgtat tcggggaacg agagcggcac
     1981 gatggtcgcc cccgcctttt tgagcgtctc gatgcaccgg tcataggcgg gtcgcaccgc
     2041 cgggcccatg ttcttttcgt attcggcgat gacaccgatg cgcatcttcg cagggtcgaa
     2101 cgcctgcgca tcgaaggagg gtgcctcgat cgaagtggca tcgcgcccat cgcgtcccgc
     2161 cgcgacctgc atcagttcgc ggatgtggcc gggacgccgc gcgaggatgc cgatctggtc
     2221 gagcgaactg gcgaacgcgg tgagtccgta gcgcgaaagg gtaccgtaag tcggcttgta
     2281 accgtagata ccgcagtagg aggccggctg gcgcaccgac ccgccggtgt cgctcccgag
     2341 cgcgatgtcg cacatcttcg ccgcgacggc ggcggccgag ccgcccgaac tgccgcccgg
     2401 ggtgcgctct tcgtcccatg gatttttcgt gtcgccgtag gccgaatagg tggtggccga
     2461 ccccatcgcg aattcgtcca gattgagttt gcccgtcacc gtcgcgcccg ccgcgacgag
     2521 ccgctcgacc accgtcgcgg tatagggggc gacataggtc tcgagcagtt tcgaaccaca
     2581 ggtgagttgc aatcccgcga ccgcgatgtt gtccttcacg ccgaggcaga ccccggaaag
     2641 cgggcccgcg ccgggcttca gccgctcttc gggaacgacc tgcaggtagg cgttgagcgc
     2701 cccgttgtgg gccgcgatgt tgccgtatat atcctttacg ccgagcgttt ccatcactcc
     2761 tcctccccct cgacgcatgc gtcgccgccg cggttcacga cccgctggac gacgaggaat
     2821 ccctccgcct cctgcgtgaa ggtgccgcgg atcgccgctt cgacccccga cggggcgggc
     2881 gcgtccttgg caagcggcag ttcgcgcagc ggcagggtga agtagggttc caccctctcg
     2941 accggcacgc gggaaaggtc ggcgaggtgt ttgaacatcc tctcgaaatg tccttccatc
     3001 gcggtcagtt cgtccttttc gccggtgagg gcggcaagtt catacaccgc gcggatatcg
     3061 tcggatggct tggtcatggg aaggatgcct ccgtatcggt ctttcaacaa gatcgttgtt
     3121 accataccgc aacgggcgac gcgaggcaaa ttttcagcaa aataggggag tatcgggcgg
     3181 cgcgaccgga aaaaggagcg gataaaattg ttgaccggcg caccgctctc ggctagagtg
     3241 accccatgga agcctatcgg atacccgaac cggtcacctt tgagaactac cccgacctgc
     3301 tcgccgccgc cttgcgcgcc atccccgccg gtaccgaggt ggtgctcgac ctcgacgggg
     3361 tgcggctcga caccgctacc aaggcgttcg tgcgccggct catcgcggtg cgcggcgacg
     3421 cggtacgggt gccggagaag tactgcgagg agatactcta cccccgcccc cgcgccgagt
     3481 cgcagaaaaa agggtccttc cggtgggccc gggtgctcga ccgggtgggc gacgccatat
     3541 tctcgctcat tgatcgtatg gtctttttcg gcgccatctt cgtcgatatg ctctattatg
     3601 ccgcgcagtc gctgtgggac cggcggcacc ttctgcgggg cgacacgatc cgcaacgcct
     3661 tccacatcgg ctacaaggcg gtcccgatcg tcgtgctcct ctcgttcctc atcgggctca
     3721 ccatctcgat acaggcggcg atacagatgg cgaaactggg tggtcagtcc tacatcgcca
     3781 cgctcatcgg tatcgcgatg ttcaccgaat tggggccgct cgtcaccgcc atcatcatcg
     3841 cgggtcgcac cgggagcgcg atagcggccg agatcgggac gatggtggtc atggaggagg
     3901 tggacgcgtt gaggacgatg ggtatccgtc cgctcaaatt catgctcgtg ccgaaattct
     3961 gggccttcac cttttcaatg ccgatactca cgctcgtcgc ctgcttcgcc ggcatactcg
     4021 gaggactcat cgtcgcgcag gtctatcagg tgccgccgag cgcgttcgtc gccgagatcg
     4081 tcaaggtcat gcggatgaag gacatcgtct ggggcgtgat aaagagtctt accttcgcgt
     4141 gggtcatcct cgccatcgcc tgctatcagg gactccatgt ccgcggcggc gccgacgcgg
     4201 tcgggcgcgc cacgaccgac tcggtggtca tctcgatatt catggtggtc gtcatcgatg
     4261 ctatctattc gctcatcctc tacacgtagg agacatcagt gaccctcttc ctgctcgtcg
     4321 gaggattcct tctcctcacc atcggcgcct cgatcctcgt cgacggcgcc gcatcggtcg
     4381 ccaagcgcta cggcatttcg aatctcgtga tcggcctgac cgtcgtcgcc ttcggtacct
     4441 cgtcccccga gatggtggtc aatgtcgttt cggcgctcag ggggaccacc gacatcgcga
     4501 tcggcaatgt gctcggttcg aacattttca atatccttgc gatactcggt ctttcggcgc
     4561 tcatccgccg cctgccggta caggaaagca ctattcgcgg ggagatcccg ttgagcctcg
     4621 tggcggcgct tctcctcttc ccgctcggcg cgccctatct gtcgttgccg ctcgatggat
     4681 cagacccgtc ggcgctggga cggatcgacg gcatcatcct gctgctgttc ttcgcctatt
     4741 tcctgcgcta caccttcaag aacgcccgta ccggcaccga aagcgacgga tcggtgaaac
     4801 tcttcagcct tcccgtgtcg gtcgcgatga tcgtcgcggg attcgcgatg ctcgttttcg
     4861 gcggcaagtt cctcgtcgat ggagcggtca tagtcgcgcg ccacttcggc atcgccgaac
     4921 gggtcatcgg gctgaccatc gtcgcggcgg ggacctcgct ccccgaactg gccacctcga
     4981 tggtcgccgc ctacaagaag aacgccgaca tcgccgtcgg caacatcgtc ggctccaaca
     5041 tcttcaacat cttcttgata ctcggtattt ccgcggtcat ccgaccgctt cccttctcga
     5101 ccggcacgac ggtcgacctg tcggtgacgg cgctctgctc catgctgttg ttcgtgttgg
     5161 tctatcgccg cactgaccgc cggctggggc gccgcgacgg tgcgctgttg gtcgcgttct
     5221 atgtcgccta tgtttcctat ctgacggtcg ggacctgagg agatcgggat ggtgacggcg
     5281 atatacatag cagggcgcgg tcgggtcggc gcggcgctcg tacgccattt cggacggcag
     5341 gcggtgccgt gccgcgtctt gaaacggggc tgggagaaac ggccccccag gcgcggcgtt
     5401 ctgttcctcg cggtgcccga cgccgcggtg gaacggatat gccgcgaggt cttcccgacg
     5461 acgccgaagg ggttgacgct cgtccatttc gccgggggtc ttcccatctc ccgtgagcgg
     5521 acgcacctcc tgcatccgtt cgcttcgatg gggcctgaga gcgagttcgg gcggctctgg
     5581 tgcctgtggc acggtccggc tgacggcggg ttcgagcgat tcttgaatac tcaccatttc
     5641 catgtgatcc gccgcgatcc gataccggga cccggctacc atgttgccgc ggtgctggcg
     5701 gggaatttcc cgcagtatct tttcctcgcg gcgcgggatc tgctccggcg cgagggtttt
     5761 tccgacgatg attcgaaacg actgctcgtc caactcgtcg agacctcggt gctcggtgtc
     5821 cgggaggggg gcgtcggtgc gctgaccggt ccggcggcgc gcggcgaccg ggcggtcatg
     5881 gaacgggaga tcgtttttct gggaaagacg gccccccggc tcgccgtcct gtatcgtgca
     5941 ctgtcggaac tcatagcgca ggcggtgggg catggcaaaa ttctcgactg atccgatact
     6001 cgaccggttc gccgaacagg tgtatgcccg tctggtgcgg gatctctgta cgccgcttcc
     6061 cgaaagtttc agggagatac cctacggcgt ttccttcaag gtcatcctac cgggcgaaaa
     6121 ggaatttccc gtcgcgctct accactccga aaagaagggc ttcagcgtcg ttacgcagag
     6181 cgaacgggtc cgccgtctca tcctcggcct gctcgccccc gaagcgctcg ccgggtgcga
     6241 cgaggcaggc aagggcgaca tcttcgggcc gctcgtcacc gcgtgtttct atctttgcga
     6301 cggttcacga ggagcgctcg aactcggggt gcgcgacagc aagaggatgg gtgacgatga
     6361 ggtgcttgca gccgctgatg aactggaccg ccgttcccgg cgttttttcg aggtggtccg
     6421 catcatgccc aagcgctata acgaactgta cgcggatttt aaaaagaagg gccggacgct
     6481 caaccacctc ctcgcgtggg cccatggcaa ggcgctttcg actctcgccg ggcggcatcc
     6541 cgacatgaga caggtgctcg tcgaccaatt cagcgacgcc ccggcgctcg tcgcgaccat
     6601 acgcgccgcc gcaccggacg cggaggtaga gttccgtccg cgggccgagg cgcatcccgc
     6661 tgtcgctctc gcctcggtcg tcgcgcgggc cgactatctg cggtcgctca agaaattgtc
     6721 tgacgagacg ctcaagggcg aattcgccct gaggagcggc tccggcgccg acgccgacgc
     6781 gcgcatcgcc gcgatccgcg cgcggcatgg cgacgcggtg ttcgccggcc tcgcgaaact
     6841 tcactttgcc aacttctcgc gggagcgctg acatggaccg
//
LOCUS       DAOQUE010000095         6827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12260_length_6827_cov_4.045481, whole genome shotgun sequence.
ACCESSION   DAOQUE010000095 DAOQUE010000000
VERSION     DAOQUE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12260_length_6827_cov_4.045481"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="PKH10_04210"
     CDS             complement(<1..1475)
                     /locus_tag="PKH10_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNT27365.1"
                     /translation="MSVDEILTSILRGAAKILGCNSANLLIFNPARQRIKAQVGIIVS
                     GKEKELRGVEKFFGVSLADAEFTFSQARDTIIYRSWKERQVIEAANLVELAGGAFSSL
                     ILKGIDTLIGHHRFIVVPVAGPVNIYGVIVFEKNGAQPFSPQQREILIGYARRIGEII
                     ENDTRSSILQERRLESDEMLARRESAVQNQLLQIALGESAPALLLDTDFRITSCNDAT
                     LTLLGYAERDLIGRDIGTLFPPSQNIHTILNHQFLFFSDGRYEEAAAVLTNEGEHLPC
                     TIEALLLADEKGHVVGFMMLLRRAAEKRPVASRDELRRQERLASMGEMAAQLAHEIRN
                     PLLAIGATLESLSADRDAPILATLSHEIQRLDMILKDYLSLAVRQNASVARTDLSLAI
                     TEVRDLLQGMQRRGDKTIALGIPPELWTLADHEGMKHVLFNLMLNALEVSPSRATVTC
                     RATADDERITITVDDEGPGLPDGDGDEADDACERRFAADFVR"
     gene            complement(1593..3389)
                     /locus_tag="PKH10_04215"
     CDS             complement(1593..3389)
                     /locus_tag="PKH10_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27366.1"
                     /translation="MFFVKFLLPITAIVLFFACSSPQENPMVDGDQIVMTDHDQLDQA
                     DQSDPSDQTDDALPPTDDDTEISDDYVKPVTCGNNVTDTGEACDGDAKNCTDISGGYT
                     GGVAVCKEDCSGYDTTACQGVADDDNAGADDEMTDADSAPDLTPDVDMNVWPECTQDG
                     ECGSSTRACYQGICRDKCTLYINECDWKPSGNICSGGLCVECLKDGDCPGTRYTCDTT
                     KLICVDKPFDPSKTKIGVFYHTWHCPSATEVHDVSKCLEQNSWPDWPSDPNVQTSFWW
                     AEPQDGYYCLTNNTALLTKHAEMLRDMGTDFVFVDVTNHNYNTSSLCINPEGMIIQPF
                     TTLVNVWKNIPGAPKIVPWVPVSDCPTYFQPNQCTHPADTYIVYTLLDILKDTGMLFE
                     YQGKPLLLITENSTYPVSETKLATLSATYTIRKMWAFEGEGTEKWSYLERCDADPLET
                     QPCFQRISKMGGVSEQFPIATAYGADYMSHTSTATPKHQGKTFRKQFQTLLDNPEIPI
                     ATITGWNEWIVGRWKCGAPACDCGNAFDAEYGCFLDQYTYEYNRDIEPAKNDPLGDYY
                     YRLVQSCIALFRAGGRCDANHAGDLCCKDYSN"
     gene            complement(3478..4317)
                     /locus_tag="PKH10_04220"
     CDS             complement(3478..4317)
                     /locus_tag="PKH10_04220"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNT27367.1"
                     /translation="MRTAIRYFSGTGNTAWLADRLKEALEKRGSPVSVGLIGIDDDPR
                     VDEGPLTLLLCFPVYALDAPPLVQRWVERIPPGEGRAVVVIRSPGDPFFNGGTTRTMR
                     RLLERRGWRIVIERMIVMPPNVFYRASDDLAALLLAAARRRIEHLADDIKEGRTLLDD
                     TGSAVHWSSSAFNGLLARHGHRFGRDLRATAACTRCGLCVRECPAGTITMTDDGIRFG
                     DRCQVCLRCMARCPARAIFPLVYRSFPVRPYDLATLDAAGPEGKKPRNWFERRYRNYL
                     DRS"
     gene            complement(4338..5606)
                     /locus_tag="PKH10_04225"
     CDS             complement(4338..5606)
                     /locus_tag="PKH10_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT27368.1"
                     /translation="MCRERLGIVLITLVFFALSCSEEKETGDESACGGTCADIATAAE
                     LTAALAGDGSCLCLLEGTYEGEFLIKRPVTLKGEGAEKTIILSQSDTPLTVRGPNDVS
                     ISDLGLKLELATLAETPALRVEARLPGPEEPGDVIQNVAVSNIAVSSVNGGGIRVTDT
                     INFSLTNATITADAKGIVLLDMTVATLSGITTQECGRMGIAFANGAQGSVVNSTILRN
                     GYTGLWIQDTSKVSVTDSTIKDNALAGINIVNAKNILIENSEIASSKELTGTPGADGI
                     VVYTPELAGDPIMAGDPIMQRAFTLLNSTIKDHPRAALVFDGTEASTGKELRNLLFSG
                     VTIFRMDYGVVFQNGLAPESFGGDSEAVSEDYSDGYDMGTSDVDTMNGGSDAAAIGDA
                     EPASDGEMPDVDTPSSGDGTLDIPSEEEEF"
     gene            5791..>6827
                     /locus_tag="PKH10_04230"
     CDS             5791..>6827
                     /locus_tag="PKH10_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNT27369.1"
                     /translation="MKNFGIDIGSTFLTIHSLQQGGVVLRRRHGGRIFEHLSSFIAEC
                     DKDVRFSFTGKGAKEFAEMTGIEPVEESLAVLRGVERADLTREGFLRLIDIGGSSLTL
                     YAFKDGRIVDIVKNALCAAGTGLFLEEQAERLAIDIESTGEMGIDDPPLVASRCTVFA
                     KSDLIHHQQEGRSRDEMWAGLCKSLVISAMNTLFRGEDLYGPIVLIGGVSLNAEVVKW
                     LKKLYPRVEWIIPKHAEAFIAMGAAQVPGTPCDELDLTKKGLDRYVKKMPALTLHKSS
                     YPKLALPEVDPDGNEIRRHRPLDGAVRVALGLDIGSTSTKLVVLDAGSLHPLFDIYRK
                     TAGDPIDATRR"
BASE COUNT         1245 a         1963 c         2212 g         1407 t
ORIGIN      
        1 cgcacaaagt cggcggcgaa acgacgctcg caagcgtcgt ccgcctcgtc gccgtcaccg
       61 tcggggagtc ccggaccttc gtcgtccacg gttatcgtta tccgttcgtc gtcggcagtg
      121 gcccgacagg tgacggtcgc gcgggaaggg ctgacctcca gcgcgttgag cataaggttg
      181 aacaggacgt gcttcatccc ctcgtggtcc gcgagcgtcc agagttccgg cggtatgccg
      241 agcgcgatgg tcttgtcgcc gcgccgttgc atcccttgca ggagatcgcg cacttcggtg
      301 atggcgagcg agagatcagt acgggcgacc gaggcgttct gccgtaccgc gagcgagagg
      361 tagtccttga gtatcatatc gagccgctgt atctcgtggg agagcgtcgc gagtatcggg
      421 gcgtcacggt cggcggaaag tgattcgagc gtcgcgccga tggcgagcag gggattgcgt
      481 atctcgtggg caagttgtgc cgccatctcc cccatgctgg cgagccgttc ctgccggcgc
      541 aattcgtcgc gcgaggcgac cgggcgtttt tccgccgcgc gccgcaggag catcatgaaa
      601 ccgacgacat ggcccttctc gtcggcgagc aggagcgcct cgatggtgca ggggaggtgt
      661 tctccctcat tggtcaggac ggcagccgcc tcctcgtaac gaccgtcgga aaaaaagagg
      721 aattggtggt taaggatggt gtggatgttc tggctcggcg ggaacagtgt gccgatatcg
      781 cggccgatca ggtccctctc ggcgtacccg agaagcgtga gcgtcgcgtc gttgcaggag
      841 gtaatgcgga aatcggtgtc gagcaggagg gcaggcgccg attcgccgag cgctatctgc
      901 aataactgat tctgaacggc gctctcgcgg cgcgcgagca tctcgtcact ctccagccgc
      961 cgttcctgca ggatggaact gcgcgtgtcg ttctcgatga tctcgccgat gcggcgggcg
     1021 taacctatga gtatctcgcg ctgttgtgga ctgaagggct gggcgccgtt cttttcgaac
     1081 acaatgacgc cgtagatgtt caccggaccg gcgaccggca ccacgatgaa ccggtgatgc
     1141 ccgatgaggg tgtctatccc tttgagtatg agcgacgaaa aggcaccgcc ggccaattct
     1201 acgaggttgg ccgcctcgat gacctgccgt tccttccacg aacgatagat gatggtgtcg
     1261 cgcgcctgcg agaaggtgaa ctcggcgtcg gcgagcgaga cgccaaagaa cttttcgacg
     1321 ccgcgcagtt ccttttcctt acccgacacg atgatgccta cctgcgcctt gatgcgctgg
     1381 cgtgcggggt tgaagatgag caggttggcg gagttgcagc cgagtatctt ggcggcgccg
     1441 cgcaggatgg atgtcaggat ctcgtcaacc gacatccggg gatgcgttct ttcttcggtg
     1501 tgtggcgtca tcgttgccat ggcgaagcga ttatatgccc ggatgtatgc ttgtcaatga
     1561 ggcggttgtt tgtacgtcat aggaagcgaa ggtcagttcg aatagtcctt acaacaaagg
     1621 tcgcccgcgt ggttggcgtc gcatcggccg ccggcgcgga acagcgcgat gcaggactgg
     1681 accagccggt aatagtagtc gccgagcgga tcgttcttgg ccggttcgat gtcgcggttg
     1741 tattcgtagg tgtattgatc gaggaagcaa ccatactccg cgtcgaaggc gttcccgcag
     1801 tcgcacgccg gagcgccgca tttccaccgg ccgacgatcc attcgttcca gccggtgatc
     1861 gtcgcgatcg gtatctcggg attgtcgagc agcgtctgga actgtttgcg gaaggtcttt
     1921 ccctgatgtt tgggggtcgc ggtgctggtg tggctcatgt agtcggcgcc gtaagcggtc
     1981 gcgatcggga actgctccga tacgccgccc attttcgaga tgcgctggaa gcagggctgc
     2041 gtctcgagcg ggtcggcgtc gcaccgttcc agataactcc acttctcggt cccttcgccc
     2101 tcgaacgccc acatcttgcg gatcgtatag gtcgccgaaa gcgtcgccag tttcgtttcg
     2161 ctgaccggat aggtgctgtt ctcggtgatg aggagcaacg gcttaccctg atactcgaac
     2221 aacatccccg tgtccttgag gatatcgagg agcgtataga cgatgtaggt gtcggcgggg
     2281 tgggtgcact ggttcggctg aaagtaggtc gggcagtcgg agaccgggac ccacggcacg
     2341 atcttggggg cgcccgggat attcttccag acattcacga gcgtggtgaa gggctggatg
     2401 atcattccct ctggattgat gcagagcgaa ctggtgttgt agttgtggtt ggtcacatcg
     2461 acaaagacga agtcggtccc catgtcacgg agcatctcgg catgtttggt caacagcgcc
     2521 gtgttgttcg tcaggcagta gtaaccgtcc tgcggctcag cccaccagaa ggaggtctgg
     2581 acattcggat cggagggcca atccggccac gaattttgct caagacactt cgacacatcg
     2641 tgcacctcgg tcgccgaggg acagtgccac gtgtgataga agacgccgat cttggtcttg
     2701 gacgggtcga acggtttatc gacgcagatg agttttgtcg tgtcgcaggt atagcgggtg
     2761 cccgggcagt cgccgtcctt caggcattcg acgcacagcc cgccggaaca gatatttccc
     2821 gacggtttcc agtcacattc gttgatgtag agggtgcatt tgtcgcggca gatgccttga
     2881 tagcaggccc gcgtggagga gccgcattcg ccgtcctgcg tgcattcggg ccagacattc
     2941 atatccacat cgggcgtaag atcgggcgcc gagtcggcgt cggtcatttc gtcgtcggct
     3001 ccggcgttgt catcgtcggc gacgccctga caggcggtgg tatcgtagcc ggaacagtcc
     3061 tctttgcaca ccgcgacgcc gccggtgtag ccgccgctga tgtcggtaca gttcttcgca
     3121 tcgccgtcgc atgcctcgcc ggtatcagtg acattgttgc cgcaggtgac cggcttgaca
     3181 tagtcgtcgg atatctcggt gtcgtcatcg gtgggcggga gggcgtcatc ggtctgatct
     3241 gaggggtccg actgatcggc ctgatccaac tgatcatggt cggtcatcac gatctgatcc
     3301 ccgtcgacca tcgggttctc ttggggggaa gaacaggcga agaacagaac gatcgccgtg
     3361 ataggcaaca aaaacttcac aaaaaacatg ggtccctccc cggtcggttt tgggaaaaga
     3421 taactctgct cttttctttg gcaaggattt tctttttgat atcgttagca gagaaagcta
     3481 cgagcggtca agatagttcc ggtaacgccg ttcgaaccag ttccggggtt ttttcccctc
     3541 cggtccggcg gcgtcgagtg tggcgagatc gtaggggcgc accgggaaac tccggtacac
     3601 gagtggaaag atggcccgcg ccggacagcg cgccatgcag cgcagacaga cctgacaccg
     3661 gtcgccgaac cggatgccgt catcggtcat cgtgatggtg ccggcgggac attcgcgcac
     3721 gcagagcccg caccgggtgc aggcggcggt cgcccgaagg tcacgaccga accggtgtcc
     3781 gtggcgcgcc aacaggccgt taaaggcgga ggacgaccag tggacggcgc tccccgtgtc
     3841 gtcaaggagc gtccggccct ctttgatgtc gtcagcgagg tgttctatgc ggcgtcgcgc
     3901 cgccgcgagt agaagtgccg cgagatcgtc gctcgcccgg tagaacacat tggggggcat
     3961 cacgatcatc cgttcgatca cgatccgcca gccgcgccgt tcaagcagac gcctcatcgt
     4021 ccgggtcgtg ccgccgttga agaagggatc gcccggcgag cggatgacga cgaccgctct
     4081 tccttcaccc ggcggtatcc gctccaccca ccgctggacg agtggcgggg catcgagtgc
     4141 gtagacaggg aagcagagga ggagtgtgag cggaccttcg tccacccgag ggtcgtcatc
     4201 gatgccgatg agtccgacgg ataccggcga tccacgtttt tcgagggcct ctttcagccg
     4261 gtccgcgagc caagccgtat tgccggtccc cgaaaaatag cgtatcgccg ttctcatgcg
     4321 ccgatcaccg gcatacctca gaattcctct tcttcgctgg gtatatcgag tgtcccgtcg
     4381 ccggaggatg gggtgtcgac atcgggcatc tcgccgtcgg aggccggttc ggcatcgccg
     4441 atggccgccg cgtcggagcc gccattcatc gtgtcgacat cggaggtgcc catatcatag
     4501 ccgtccgagt aatcctcgct gaccgcctcg ctgtcaccgc caaaactctc gggggcgagg
     4561 ccgttctgga agacgacgcc gtagtccatc cggaatatcg tgactccgct gaacagcagg
     4621 ttgcggagtt ccttgccggt cgacgcttcg gtgccgtcga atacgagcgc tgcgcgcggg
     4681 tggtccttga tggtcgagtt gaggagcgtg aaggcgcgtt gcatgatcgg gtcgcccgcc
     4741 atgatcgggt cgcccgccag ttccggggtg tagacgacga tgccgtcggc gcccggcgtg
     4801 ccggtcagtt cctttgatga ggctatctca ctgttctcga tgaggatgtt cttagcgttg
     4861 acgatattga tgcctgccag cgcgttatcc tttatggtcg aatcggtcac gctgaccttg
     4921 gaggtgtcct gtatccagag accggtatag ccgttgcgca gaatggtcga gttgaccacg
     4981 cttccctgtg cgccgttggc gaaggcgatc cccatccggc cgcattcctg cgtcgtgatg
     5041 cccgacagcg tggcgacggt catgtctagg aggacgatcc ccttagcgtc ggcggtgatc
     5101 gtggcattgg tcaatgagaa gttgatggtg tcggtcacgc ggatgccgcc gccgttcacg
     5161 gaggaaacgg cgatgttcga tacggcgaca ttctggatga catcgcccgg ctcctcgggg
     5221 cccggcagtc tggcttccac ccgcagggcg ggggtctcgg cgagcgtcgc gagttccagt
     5281 ttgagaccga gatcggaaat actcacatcg ttcggacccc gcaccgtaag cggtgtgtcg
     5341 ctctgcgaga ggatgatagt tttttccgcc ccttcgcctt tgagggtgac cggtcttttg
     5401 atgagaaatt ccccttcata ggtcccttcg agcaggcaca ggcaggaccc gtcgcccgcg
     5461 agcgccgcgg taagttcggc cgcggtcgcg atgtcggcgc aggtgccgcc gcaggcgctt
     5521 tcatcgccgg tctccttctc ttcagagcag gagagagcga agaagaccaa agttatcagg
     5581 acgataccca accgctcacg acacatggca cacctccgtt aacgtcgttc ggcccctttg
     5641 gtgttcagta tgtcccgttt ttattgaaaa agcaaaaaca tccttcgggg gcagcggtgc
     5701 gcaatcgcac aattaaaaaa gttgaaatgg ccatgtgccg tcggtatagt gcccgcgctt
     5761 gtggttctct tcaccattaa tgaggttgag atgaagaatt tcggcatcga catcggatcc
     5821 actttcctga ccatccattc gctccagcag gggggcgtcg tcctccggcg tcgtcacggc
     5881 gggcgtatat tcgaacacct ctcctcgttc attgccgaat gcgataagga cgtacggttc
     5941 tcgttcaccg gcaagggggc gaaggaattc gccgagatga cggggatcga accggtcgaa
     6001 gaaagtctcg cggtgttgcg cggcgtggaa cgtgccgatc tgacacgcga ggggttcctg
     6061 cggctcatcg acatcggcgg ctcgtcgctg acgctctacg cgttcaagga cgggcgcatc
     6121 gtggatatcg tcaagaacgc actgtgcgcc gccgggaccg gcctgttcct cgaagagcag
     6181 gcggaacggc tcgctataga catcgagagc accggcgaga tggggataga cgacccgccg
     6241 ctcgtggcga gccggtgcac cgtcttcgcc aagagcgatc tcatccacca tcagcaggaa
     6301 gggcggtcgc gcgacgagat gtgggcggga ctctgcaagt cgctggtcat ctcggcgatg
     6361 aacacgctgt tccgcggcga ggacctgtac ggccccatcg tcctcatcgg cggggtctcg
     6421 ctgaatgccg aagtggtcaa gtggctgaag aaactgtatc cgcgggtcga atggatcatc
     6481 ccgaagcatg ccgaggcatt catcgcgatg ggagcggcgc aggtgccggg gacgccgtgc
     6541 gacgaactgg atctcaccaa aaaagggctc gaccggtatg tcaaaaagat gccggcgctc
     6601 accctgcaca agagttccta tccgaaactg gcgcttcccg aagtggaccc ggacggcaac
     6661 gagatacggc ggcaccggcc gcttgacggc gcggtccgtg tggcgctcgg gctcgacatc
     6721 ggttcgacca gcaccaaact ggtcgtgctc gacgccgggt cgctccaccc gctattcgac
     6781 atctaccgca aaacggcggg tgacccgata gatgcgacgc gccggat
//
LOCUS       DAOQUE010000096         6815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12284_length_6815_cov_3.677219, whole genome shotgun sequence.
ACCESSION   DAOQUE010000096 DAOQUE010000000
VERSION     DAOQUE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12284_length_6815_cov_3.677219"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PKH10_04235"
     CDS             <1..725
                     /locus_tag="PKH10_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNT27370.1"
                     /translation="RMYEKEGTTVSKYAADVALTRDNLKSEWPKGYGMVFWFGHGLDK
                     EVARTIWIDANDDGEVSNDELQQPLFIHSDDAPAIGAQSPAFVVAVSCEVGSAQTPWN
                     LTHALLTEGGAIGMIGSTNVTPGDMTDYGDFESELDTSTYGATNLGVFFFDELMNGAV
                     AAQAFAEVRRDVGTSPSIETYAGKMMLNYYGDPTLSLTSSAADAPDEELTPDEDTATD
                     ADSPDTDTAEKGSDGGCALMVL"
     gene            complement(743..1357)
                     /locus_tag="PKH10_04240"
     CDS             complement(743..1357)
                     /locus_tag="PKH10_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27371.1"
                     /translation="MPTAFRILSFLILSLVVSLLFADEAPPYRPYDRESANKQYVAKV
                     TIHEEDKDKNRYDARWVLTIYKKTAKGEEKLWTVPFFHRGYWGGALSDDGQVLVDAEQ
                     WYYHHEPAVIVYSKGKEVGRILGKDFGVPESKREKTESHELWLSENSFSTELRRENEF
                     VKTTDGYDYKIYTIDGNIHLIDCKTGKIKKTQKQEPETVPSSKK"
     gene            complement(1366..2868)
                     /gene="malQ"
                     /locus_tag="PKH10_04245"
     CDS             complement(1366..2868)
                     /gene="malQ"
                     /locus_tag="PKH10_04245"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNT27372.1"
                     /translation="MTTLHRSSGIILHPAALPGPGPIGTLGDAARRFVDWLATGKQGL
                     WQILPLTPTSYDGCPYNAYSAFAGNPWLIDPDLLVREGFIADAGPYRIPAPDPRRVNF
                     DMARDQSDAMLREAFLRFHESRERFEEFSAFRTANSHWLGDHALYAALRERFGTSCWH
                     EWPVEFRERRPAAMARLMHEAADDILRHQFAQWLFLRQWRSLRAYANERGVRIVGDLP
                     MFVAYESADVWAHQDLFLLKDGRPTVVAGVPPDYFSPTGQLWGNPLYDWKRMEEDGFD
                     WWKRRFAQMFALYDLVRIDHFRGFAASWAVPAGDTTAENGKWRKTPGRKLFDALLAQF
                     GELPVIAEDLGVISDDVVELRERFNFHGMKVLQFGFDAPTNAFLPHNYTTPNCAVYTG
                     THDNDTVAGWYRSLYEHDRREINRYLGLRWGDSPAWALIRLAHGSVARYAFTTLQDVL
                     GMGSEARFNTPGTVGLHNWTFRFTHDDIRDEAAWGLAELSWLYNRNEHSE"
     gene            complement(3025..4218)
                     /locus_tag="PKH10_04250"
     CDS             complement(3025..4218)
                     /locus_tag="PKH10_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNT27373.1"
                     /translation="MYHLLSFSGIFVFVFLAWLISEHRRRFPWRVVVWGIVIQVLFAL
                     FILRTAPGAWLFDLVNRVFSKMMQFTDAGSEFVFGGLLKEWSFALKILPTIVFFSSLF
                     TVLYHFGVLQKVVKGFAFLFRRLLGTSGAESLSAAANIFIGQTEAPLMIRPYVETMTR
                     SELMCVMSGGMATIAGGVMAAYVGMGVSAGHLMAASVMSAPAAIMFAKIMVPETGTPV
                     TVGGGTIDIPVVDRNAIEAAARGASEGLFLALNVAAMLLAFIAFVAMFNYFFGFFGVT
                     MEQVLGWAMAPLALCMGVPWDEAMRVGAFLGEKTILNEFVAYAHLSDAIKAGATELSP
                     RSVIIATYALCGFANLGSIAIQIGGIGTMAPSRRGEIAALGIKSMIAGTLASFVTANI
                     AGMLI"
     gene            complement(4294..4782)
                     /locus_tag="PKH10_04255"
     CDS             complement(4294..4782)
                     /locus_tag="PKH10_04255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT27374.1"
                     /translation="MEITHKVLLETNKGNITLGLYGKETPVTTANFVKYVEAGFYSKL
                     LFHRVIEDFMIQGGGMIVGLFPKKDLFPPIKLEIAPQIKHEKYTLSMARTSDPNSATC
                     QFFICTARCDSLDGQYAAFGTVLEGKEVVDAIAAVETHSVRHYDDVPVENVVIAKASI
                     IP"
     gene            4954..6561
                     /locus_tag="PKH10_04260"
     CDS             4954..6561
                     /locus_tag="PKH10_04260"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNT27375.1"
                     /translation="MSVPRIAVLIAAFFFIAACDGVKTIKPKDDDVLSGDDDSLLSDD
                     PATDDLTTDTAGTDDTVTDTNVNDDTITDTPPVDNDITPVTCGNGDIDLGEICDKSTG
                     TLANCTDINSDWYQGGKAYCQDDCLGWDTSTCEEVPHTCGNNTPEGPEVCDGGSTPCA
                     TLSPEKYQSGTAYCNGDCDGWLTDGCVEIEPSECKDGKVEGIEVCDGGPDGKANIADC
                     VNIDPLLYSGGKAYCNANCDGWLVETCTERPPECESTLNLASKDPYHYAKAIGICSGV
                     PKAEMLLPSGSTGANATGHALLAALGEVITPRGGDKMMVLSSGQAMDPPDNTSFDQQT
                     SSTAPADWYADNGNKYPSSPACGGTTGTTGNTNDGVMARFEVDVPSTARSFSFDIYFL
                     SVEYPSYVCSAYNDLFVALLDSSFTTTVETQKNPIDGNLAVYEGNAVGVNLAPAGLFT
                     QCTNTSGTGWEVTSCEGTEELAGTGFASNGGTGWLTVRGNVVPNETMTLRLAIWDLGD
                     HILDSMVLIDNFKWGTTNVTPGMTKAQ"
     gene            6573..6767
                     /locus_tag="PKH10_04265"
     CDS             6573..6767
                     /locus_tag="PKH10_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27376.1"
                     /translation="MPAPLPSRTGRLLRVIARLLLLAVAVLLIARAFTIPIDGDGTRE
                     ITLVRTNGSTFCQSCMGLYK"
BASE COUNT         1432 a         2160 c         1988 g         1235 t
ORIGIN      
        1 cgcggatgta cgaaaaagag ggaacgaccg tgtcaaaata cgcggccgat gtggcgctga
       61 cgcgcgacaa cctgaaaagc gaatggccca agggctacgg gatggtcttc tggttcggcc
      121 acgggctcga caaggaggtg gcgcggacga tatggatcga cgcgaacgac gacggagagg
      181 tatccaacga tgaactgcag cagccgcttt tcatccattc ggacgacgcc cccgccatcg
      241 gcgcgcagag ccccgcgttc gtcgtggcgg tctcgtgcga ggtgggcagc gcacagacgc
      301 cgtggaacct gacccatgcg ctcctcaccg agggcggcgc gatagggatg atcggctcga
      361 ccaatgtcac gccgggcgac atgaccgatt acggcgattt cgagagcgaa ctcgacacct
      421 cgacctatgg cgcgaccaac ctcggcgtct tcttcttcga tgaactgatg aacggcgcgg
      481 tggcggcgca ggcgttcgcc gaggtgcgac gcgatgtggg gacctcgccc tccatcgaga
      541 cctacgcggg gaagatgatg ctcaactact acggcgaccc gaccctgtcg ctcaccagtt
      601 ccgccgccga cgccccggac gaagaactca cccccgatga agacaccgct accgacgccg
      661 attcgcccga caccgacacc gccgaaaaag gatcggatgg cgggtgtgcg ttgatggtcc
      721 tgtaaagatt gtcagccaaa aatcactttt ttgaagaggg cactgtttcg ggttcctgtt
      781 tttgtgtctt ctttatcttt cctgtcttgc agtcaatgag atgaatattt ccatcgatag
      841 tgtaaatctt gtaatcatac ccatccgtcg ttttcacaaa ttcgttttcc cttctcaact
      901 cggtcgaaaa cgaattttca gatagccata attcatgaga ttccgttttt tctcgtttcg
      961 attccggcac accaaaatct ttgcccagta tccgcccaac ctctttccct ttgctgtaaa
     1021 cgatcactgc gggctcatga tgataatacc attgttcggc atctaccaaa acctgaccat
     1081 catcggataa tgctccgccc caataacctc tgtggaagaa tggcacggtc cataattttt
     1141 cttctccttt ggcggtcttt ttataaatcg tcaaaaccca tcgtgcatca taccgattct
     1201 tgtctttatc ctcttcatgt atggtaacct tcgcaacata ctgtttatta gcagattctc
     1261 tatcatatgg tctatagggt ggtgcctcgt cggcaaaaag taaacttacc accaatgaca
     1321 gaatcaaaaa cgatagaatg cgaaaggcgg taggcatctt ttatttcact cgctgtgctc
     1381 attccggttg tagagccacg agagttcggc gagtccccac gccgcctcat cgcggatgtc
     1441 gtcatgcgtg aaccggaagg tccagttgtg gagcccgacg gtccccggcg tattgaaccg
     1501 cgcctcagac cccatcccca gcacatcctg cagggtcgtg aaggcatacc gcgcgacact
     1561 gccgtgagcg agccgtatga gcgcccacgc cgggctgtca ccccaccgca gaccgagata
     1621 gcggtttatc tcgcggcgat cgtgctcgta cagcgaccgg taccagccgg ccaccgtgtc
     1681 gttgtcgtgc gtcccggtat agaccgcgca attcggggtc gtgtagttgt gcggcagaaa
     1741 ggcattcgtc ggtgcatcaa agccgaactg gagaactttc atcccgtgga aattgaaccg
     1801 ctcgcgcagt tccaccacat catcggatat gacgccgagg tcctcggcga tgaccggcag
     1861 ttcaccgaac tgggcaagga gcgcgtcgaa cagtttcctg cccggcgtct tacgccattt
     1921 gccgttctcg gccgtcgtgt cgcccgccgg caccgcccaa gaggcggcga acccgcggaa
     1981 gtgatcgatg cggacgagat cgtagagggc gaacatctgc gcgaaccgcc gcttccacca
     2041 atcgaacccg tcctcctcca tccgtttcca gtcgtacagc gggttgcccc acaactggcc
     2101 ggtcggcgag aaataatcgg gcggcacccc cgcgacgacg gtcggacggc catccttgag
     2161 caggaacagg tcctgatggg cccacacatc ggcgctctcg taggcgacga acatcggcag
     2221 gtcgccgacg atgcggaccc cccgctcgtt ggcgtaggcg cggagcgatc gccactgacg
     2281 caggaaaagc cactgggcga actggtgacg caggatatcg tcggccgctt cgtgcatgag
     2341 ccgcgccatc gccgccggtc gccgctcgcg gaactcgacc ggccactcgt gccagcacga
     2401 ggtgccgaac cgctcgcgga gcgcggcata gagcgcgtga tcgccaagcc agtgactgtt
     2461 cgcggtccgg aacgcgctga actcctcgaa ccgctcgcgg ctctcatgga accgcaggaa
     2521 cgcctcgcgc agcatcgcgt cgctctgatc ccgcgccata tcgaaattga cccggcgcgg
     2581 atcgggtgcc ggtatccggt acggcccggc gtcggcgatg aagccctctc gcacgagcag
     2641 gtcggggtct atcagccacg gatttccggc gaaggccgag taggcgttgt agggacagcc
     2701 atcataggag gtcggcgtaa gcggcagtat ctgccagagc ccctgcttgc cggtagcgag
     2761 ccagtcgacg aaccggcgcg ccgcatcgcc gagtgtgccg atgggaccgg ggcccggcag
     2821 ggccgcaggg tgaaggatga tgccgctgga acgatgaaga gtggtcatag tcctactcca
     2881 tacagagatc gttgccgtga ctttccaacc tcaccccgac cctctccatc gtgtggagag
     2941 ggagaattcg gtcgcctttt ttcccttctc cgcacgacgg agaaggtggc cgcaggccgg
     3001 atgaggttga agacaaaaag gcgttcatat cagcataccg gcgatattgg cggtcacgaa
     3061 gctggcgagc gtccccgcga tcatcgactt gataccgagc gccgctatct cgccgcgccg
     3121 cgacggggcc atcgtcccga tgccgccgat ctgtatcgct atcgagccga gattcgcgaa
     3181 gccgcagagc gcataggtgg cgatgatgac cgaccggggc gacagttcgg tcgcgcccgc
     3241 ttttatcgcg tcggaaagat gggcataggc gacgaactca ttgagtatcg tcttttcgcc
     3301 gagaaaggcg ccgacacgca tcgcctcgtc ccacggcacc cccatgcaga gagcaagcgg
     3361 cgccatcgcc cagccgagca cctgttccat cgtgacgccg aaaaaaccga aaaagtagtt
     3421 gaacatcgcg acgaacgcga tgaaagcgag cagcatcgcc gcgacattga gcgcgaggaa
     3481 cagtccctcc gacgcgccgc gcgccgccgc ctcgatggcg ttgcgatcga cgaccgggat
     3541 atctatcgta ccgccgccga cggtcaccgg cgtaccggtc tcggggacca tgatcttggc
     3601 gaacatgatc gcggcgggag ccgacatgac cgacgccgcc atcaggtgcc ccgcgctcac
     3661 ccccatcccc acataggccg ccataacgcc ccccgcgatg gtcgccatgc cgcccgacat
     3721 gacgcacatc agttccgagc gggtcatcgt ttcgacgtag ggccgtatca tgagcggtgc
     3781 ctcggtctgg ccgatgaaga tgttggcggc ggccgagagc gactcggcgc ccgaggtgcc
     3841 cagcaaccgg cgaaaaagga acgcgaagcc tttgaccacc ttctgcagga cgccgaagtg
     3901 gtagagcacc gtgaacagcg acgagaagaa gacgatggtg ggcagtatct tgagcgcaaa
     3961 ggaccattcc ttgagcaggc cgccgaagac gaactcgctc ccggcgtcgg tgaattgcat
     4021 catcttggaa aaaacgcgat tgacgagatc gaacagccat gcgccggggg cggtccggag
     4081 aatgaagagc gcgaagagca cctgtatgac gatcccccac acgaccacgc gccacgggaa
     4141 ccgcctgcga tgctccgaga tgagccacgc aaggaacacg aacacgaaga tgccggaaaa
     4201 actcaggaga tgatacatca cgctactccc cgacaggtcc ggaggcatta tagcggacgg
     4261 ggaacggaag gcaaatttaa ccggggggcg cgctcagggg atgatcgacg ctttggcgat
     4321 gacgacattt tcgaccggca catcgtcgta gtggcggacg ctgtgggtct cgaccgcggc
     4381 gatggcgtcc accacttctt tgccctcaag caccgtgccg aacgcggcgt attgcccgtc
     4441 gagcgagtcg caccgcgcgg tgcagatgaa gaactggcag gtggcactgt tcggatcgct
     4501 cgtgcgggcc atcgagagtg tgtatttctc gtgttttatc tggggggcga tctccagttt
     4561 tatcggcggg aacagatctt ttttcgggaa caggccgacg atcatgccgc caccctgtat
     4621 catgaagtcc tcgatgacgc gatggaagag cagcttgctg tagaaccccg cctcgacata
     4681 cttcacgaag ttcgccgtcg tgaccggcgt ttccttaccg tacaggccga gcgtgatgtt
     4741 acctttgttc gtttcgagca gtaccttgtg cgtgatctcc atagccgtcc tcctttcaaa
     4801 cgggtatcgg tagcgaaacc ataccgaccg tcaccggaaa aacaagaaaa aatagcaggt
     4861 ttcaccgctt tgacttgaca ccgtcacccg tatcgctatc atggcggcgg gtatcggagg
     4921 gttttgtaga cgcagaacat aacggaggga gccatgagcg taccgcgcat cgccgttctt
     4981 atcgccgcct ttttcttcat cgccgcctgc gacggcgtga aaacgataaa accgaaggat
     5041 gacgatgtcc tgtcgggcga cgacgactcg ctcctcagcg acgacccggc gaccgacgac
     5101 ctgaccaccg acaccgccgg gaccgacgac acggtcaccg acaccaacgt gaatgatgat
     5161 acgatcaccg acaccccgcc ggtcgacaac gacatcacgc cggtgacctg cggcaacggc
     5221 gacatcgacc tcggcgagat ctgcgacaag agcaccggga cgctcgccaa ctgcaccgat
     5281 atcaacagcg actggtatca gggcggcaag gcctactgtc aggacgactg tctcggctgg
     5341 gacacgagca cctgtgaaga ggtcccgcac acctgcggca acaacacccc cgagggaccg
     5401 gaggtgtgcg acggcggctc caccccctgc gccacgctca gccccgagaa atatcagagc
     5461 gggaccgcct actgcaacgg cgactgcgac ggctggctca ccgacgggtg tgtcgagata
     5521 gagccgtccg aatgtaagga cggcaaggta gaggggatcg aggtctgtga cggcggaccc
     5581 gacggcaaag cgaatatcgc cgactgcgtc aacatcgacc cgctcctcta cagcggcggc
     5641 aaggcctact gcaacgccaa ctgcgacggc tggctcgtcg agacctgcac cgagcggccg
     5701 cccgaatgcg agtcgacctt gaacctcgcc tcaaaggatc cctaccacta cgcgaaggcg
     5761 atcgggatat gttcgggggt gccgaaggcc gagatgctcc tcccgagcgg gagcaccggc
     5821 gccaatgcga cgggacacgc gctccttgcc gcgctcggcg aggtcataac gccgcgcggc
     5881 ggcgacaaga tgatggtcct ctcatcgggt caggcgatgg atccgccgga caacaccagt
     5941 ttcgaccagc agacctcctc gaccgccccg gccgactggt acgccgacaa cggcaacaaa
     6001 tatcccagtt ccccggcctg cggcggcacc accggcacga ccggcaacac caatgacggc
     6061 gtcatggcgc ggttcgaggt ggatgtccct tcgaccgccc gctccttcag tttcgacatc
     6121 tatttcctgt cggtcgaata tccctcatat gtctgttcgg cctataacga cctgttcgtc
     6181 gcgctcctcg acagttcctt caccacgacc gtcgaaacgc agaagaaccc gatcgacggg
     6241 aacctcgcgg tctacgaggg caacgcggtc ggcgtcaatc tcgccccggc gggactcttc
     6301 acccagtgca ccaatacctc gggcaccggc tgggaggtca cctcgtgcga ggggaccgag
     6361 gaactggccg gcaccggctt cgcatcgaac ggcgggaccg gctggctcac ggtacgcggc
     6421 aatgtcgtgc cgaatgagac gatgaccctg cgactcgcga tatgggatct cggcgaccat
     6481 atcctcgact cgatggtcct catagacaat ttcaagtggg gcacgacgaa tgtgaccccc
     6541 ggcatgacca aggcccagtg acggtacccg ccatgcccgc tcctctcccc tcccggaccg
     6601 gacgcctcct gcgggtcatc gcccgccttc tcctcctcgc ggtcgccgta ctgctcatcg
     6661 cacgggcctt caccatcccg atagacgggg acggcacccg cgagataacc ctcgtccgga
     6721 ccaacggctc gaccttctgt caatcctgca tgggacttta caagtgagac gctggctcgc
     6781 ccagttcctt ttccttttca tccagaaccc cttca
//
LOCUS       DAOQUE010000097         6783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12357_length_6783_cov_4.340517, whole genome shotgun sequence.
ACCESSION   DAOQUE010000097 DAOQUE010000000
VERSION     DAOQUE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12357_length_6783_cov_4.340517"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="PKH10_04270"
     CDS             <1..1060
                     /locus_tag="PKH10_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HNT27377.1"
                     /translation="GVYDGSYMRIYVDGTQRSAQQCASTAAPAQTDAAPPSGDTNPLY
                     IGYVLPGGYPYMSGYIDEVRIYKGKALSPAEILSDWDNRYIMEACDDAGCKADCSGPA
                     CTASTYTFTTVGSTTWAVPVDGNYQIEAWGANGGQYSSGRGIGGYAKGTTALSGTVYV
                     YVGGAGTYAAGSCTGGVNGGGYAYGYTSGAYASSGGGASHVATASGTLSSLVSNKTAV
                     KIVAGGGGGAGYYSTTAYVGGAGGGTTGGTGTTYSGYTTAGGGTQSAGGAAGTSSSYS
                     QAGSFGQGGNQTATSGNWNACGGGGGWYGGGSGAAVGSGGGGGSSYVTGLSNASTLSG
                     QDAGNSNNGKVVITRVCP"
     gene            1126..5454
                     /locus_tag="PKH10_04275"
     CDS             1126..5454
                     /locus_tag="PKH10_04275"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT27378.1"
                     /translation="MSSLKLSPLFLLLLFAALFTACEESPKKEDPFKQGSLWGPCYDD
                     GTCDAGLVCKEDVCWEASDSDLLTDDLIPDEEEDDAKTDTDKVDTDKVDTDTTDVDAK
                     DTDTTDDDVVTGPCAADPCAAVEHSDGVCTVDGDDYTCGCIEHYSWDGDNKLCAADIQ
                     RIDCVNIPDNAHGIGDNADGKFEQEWDGDSWEPVSFFCEWVCDDNYALNEDEDGCIGA
                     TRRVDCADIPDNAHGTGDNADGKFAQTWDGDSYEPATVTCTWACDLNYTWDAGNELCL
                     ADTRRADCTNIPANGHGTGDNADGKIDQTWGGSAWLPAADTCTWECDLNYTANGQVCD
                     ADTQRVDCTNIPANAHGTGDNSDGKFLQTWDGSDWLPGSVVCTWACDTNFTENPDGDA
                     CVADTRHAPCANIPDNAHGINDNDQGTFRQTWDGDSWEPATYACEWDCDEHFTRVNDL
                     CEADTQRVACTNIPANAHGTGYNFDGKFEQEWSGTEDMWLPETFECTWECDTNYTQNG
                     NACDANTREADCANTLPAHAGWSGDNENGTFTQTWSSADNDWLPTTFDCLWTCDENYS
                     LNAAEDGCDADERIVDCEGTPPEHASWSGDNADGKITQVWDGDSWEPAATTCTWACDE
                     NYSLNDTEDGCDPDTRRVDCENAIPEHAAYSGANDDGQFAQTWDGDSWEPATFECAWV
                     CAYQYEWNEGTELCDLRPVIYVDLNATDGANTGYSWADAFVDLQDALDSIVDGQEIWV
                     AAGIYTPTACPYYVTCTEKQKSFNLVNRRSDMIHYRLYGGFDGTETARDERDWAAHET
                     ILSGDMSGDDAWDDLNGAWTNREDNTYHVLLFDPLPIEDALTIDGFTVTGGEATGEEG
                     EQRDGGGFSLSNHTVTIRNLTFIGNSAAVNGNALSCNGCDVTVEDSYFYRNIFPDGTT
                     GGGTVSVSGTLSIARVTFEENRGPVGAVSFNAPTGGTLIVADTDFLDNSASVGTAAIS
                     GAGGAAILTGCAFIGNVGYAAGAVALNNVESAMLDACYFENNAASSIEPGNEGSAGAL
                     SVSEGSFSITDSQFIGNTAYRNAAISLYNIGNATVLGVDFIDNIGYTNGALGAPTTVL
                     TVGGCVFSGNEATSNEEGKWGNGGAISCGEGALTVTNSTFSGNSAFTGGVIQAHNAAL
                     SISNSSFDANVTHAPDQGGNVSADAGTAELLIEGSSFTNNLGAAVTAGGGQLVQIFGS
                     LFSGNHAVNSAAIAVQSGSSAIIADCRFIDNAADAPGEGFAGLGGAIMVNGMDDETGG
                     PVEGVDIWIANSVFAGNRANLWGGAIFSLLLHPVITSCTFHDNLDSGNDAIVFVMSDA
                     VIDNSVIWDKMSVLEGEVGPFNFGPSNPTFSYSDVRYSGGSGNDCGGGSDPCWVLPFG
                     TDGGGNIDADPLFVGSGDDPFDLQGTSPCIDAGSNALVPEGIDFDILGDARIQNTAVD
                     MGAYEQ"
     gene            5671..6057
                     /locus_tag="PKH10_04280"
     CDS             5671..6057
                     /locus_tag="PKH10_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT27379.1"
                     /translation="MLDVALIMPLLKKERRILAAYLLGSAVNGTLRPDSDIDIALMFL
                     PGKGMTGIERGELAAELSLILRREVDLGVISSGNLVYAREAILTGRRIFSRNDGIADL
                     YAATLLGLYAQFHYERREILHGYLAR"
     gene            6038..6469
                     /locus_tag="PKH10_04285"
     CDS             6038..6469
                     /locus_tag="PKH10_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNT27380.1"
                     /translation="MAILPDDVILNKAAVIERALRRMREEYAFDPELKSFTHIDAMTL
                     NIERAAQAAIDLAMHLVSRDRLGAPQTSGDAFRLLAEAGVVSPETLRAMTAMIAFRNI
                     AVHEYQEMDLSVLRNIAGDRWRSLVDYCAELGITITVDRGK"
BASE COUNT         1388 a         1985 c         2105 g         1305 t
ORIGIN      
        1 cggtgtctat gacgggtctt atatgcggat ctatgtggac ggtacgcagc ggagcgctca
       61 gcagtgcgcg tcgaccgccg cgcccgcgca gaccgatgcg gcgccgccgt cgggcgacac
      121 caatccgctc tacatcggct atgtgctccc cgggggctac ccgtatatgt cggggtacat
      181 cgacgaagtg cgcatctaca agggcaaggc gctttcgccc gccgagatcc tgtccgactg
      241 ggacaatcgg tacattatgg aggcgtgcga cgatgccggc tgtaaagccg actgttccgg
      301 cccggcctgt accgcctcga catacacatt caccacggtg ggctccacta cttgggcagt
      361 gcccgttgac ggcaattacc agatagaggc gtggggtgcg aacggaggtc agtatagttc
      421 cggtcgcggt atcggcggtt acgcaaaagg gacaacggcc ctatccggaa cagtatatgt
      481 ctatgtcggt ggcgcaggca cctacgcggc aggttcctgc accggagggg ttaacggtgg
      541 cggttacgca tacggctata cctccggcgc atatgcctca agtggcggcg gagcctccca
      601 tgtggctact gccagcggca ccctctcctc cctagtcagt aacaagaccg cggtaaaaat
      661 cgttgccggc ggcggcggtg gcgccggata ttattccacg acagcgtatg ttggcggtgc
      721 cggtggcggg accacaggag gaactgggac cacatactcg ggctatacaa cggcaggcgg
      781 agggacgcaa tcagccggag gtgccgcagg cacaagtagc tcttattctc aggccggatc
      841 ttttggacag ggaggcaatc agaccgcaac ctccggtaac tggaacgcct gtggcggcgg
      901 cggcggctgg tacggcggtg gatccggcgc ggcagtcggg agcggcggcg gtggtggctc
      961 gtcttatgtt accggcctca gcaacgcctc aacgctttcc ggccaggatg ccggcaattc
     1021 aaacaacgga aaagtcgtca ttacccgggt gtgtccgtag cgtagagtat gaaagggttt
     1081 aggtatattg tcgtttgttc aacaaccatc catgaggagg tatccatgtc ttcgctgaaa
     1141 ctgtcccccc tgttccttct gcttcttttc gcggcgcttt tcaccgcctg cgaggagagc
     1201 cccaagaaag aggacccgtt caaacagggt tcgctctggg gcccctgcta cgatgacggc
     1261 acctgcgacg cggggctcgt ctgcaaggaa gatgtctgct gggaggcgag cgacagcgat
     1321 ctcctgaccg acgacctcat ccccgacgag gaggaggatg acgcgaagac cgacaccgat
     1381 aaggtggaca ccgacaaggt cgataccgat acgaccgatg tcgatgcgaa ggacaccgat
     1441 acgaccgacg acgatgtcgt gaccggcccc tgcgccgccg acccctgcgc ggcggtcgaa
     1501 cattccgacg gtgtctgcac cgttgacggc gacgactata cctgcggctg tatcgaacac
     1561 tactcgtggg atggcgataa caaactgtgc gccgccgaca tccagcgcat cgattgcgtc
     1621 aatatccccg acaacgctca cggtataggt gacaacgccg atggcaaatt cgaacaggaa
     1681 tgggacggcg attcatggga accggtatcg ttcttctgtg aatgggtgtg cgacgataac
     1741 tacgcgctga acgaggatga ggacggttgt atcggtgcta cgcgccgagt ggactgcgcc
     1801 gatatccccg ataacgcgca cggcaccggc gacaacgccg acggcaagtt cgcgcagaca
     1861 tgggacggcg acagttacga gccggcgacc gttacctgta catgggcgtg cgacctgaac
     1921 tacacatggg atgccgggaa cgagctctgt ctcgccgaca cccggcgtgc cgactgtacg
     1981 aacatcccgg cgaacggtca cggtaccggc gacaatgccg acggaaagat agaccagaca
     2041 tggggcggct cggcgtggtt gcccgccgcc gatacctgta cttgggaatg tgacctcaac
     2101 tataccgcga acggtcaggt ctgcgatgcc gacacccagc gggttgactg cacgaacatc
     2161 ccggcgaacg cccacggcac cggcgacaac agcgacggca agttcctcca gacatgggac
     2221 ggctcggact ggcttcccgg ctcggtcgtc tgtacatggg cgtgcgacac caactttacc
     2281 gagaatcccg acggcgacgc ctgtgtcgcc gacacgcgcc acgccccctg cgccaatatc
     2341 cccgataacg cccacggcat aaacgataac gatcaaggca cctttcggca gacatgggac
     2401 ggcgacagtt gggaaccggc gacctacgcc tgtgaatggg attgcgacga gcattttaca
     2461 cgggtcaacg atctttgcga ggccgatacc caacgcgtag cctgcacgaa catcccggcg
     2521 aatgcccatg gcaccggtta caactttgac ggcaaattcg aacaggaatg gagcggcacg
     2581 gaggatatgt ggcttcccga gaccttcgag tgcacttggg aatgcgacac caactatacc
     2641 cagaacggca acgcctgcga tgcgaacacc cgtgaggcgg attgcgccaa cacccttccg
     2701 gcccacgcgg gttggagcgg ggacaacgag aacggcacct tcacccagac ttggagcagt
     2761 gccgacaatg actggctgcc gacgaccttc gattgtttgt ggacctgcga cgagaattat
     2821 tcgctcaatg ccgccgagga cggctgtgat gccgacgagc ggatcgttga ctgtgaaggt
     2881 acgccgcccg aacatgcctc gtggagcggc gataacgccg acggcaagat aacgcaggta
     2941 tgggacggcg acagttggga accggcggcc accacctgca cttgggcatg cgacgagaac
     3001 tattctctga atgacaccga ggacggctgt gaccccgaca cccgtcgcgt cgattgcgag
     3061 aatgctatac cggaacacgc ggcatactcc ggcgccaacg acgatggcca gttcgcccag
     3121 acatgggacg gcgactcgtg ggagcccgcc accttcgaat gcgcttgggt ctgtgcgtat
     3181 cagtacgaat ggaacgaagg gaccgaactc tgtgacctgc gtcccgtcat ctatgtggat
     3241 ctgaacgcga ccgatggcgc gaacaccggg tattcatggg ccgatgcgtt cgtcgatctg
     3301 caggacgcgc tggattcgat cgtcgacggt caggagatat gggtggccgc cggcatctac
     3361 acgccgaccg cctgccccta ctatgtcacc tgcaccgaaa aacaaaagag cttcaatctc
     3421 gtcaaccgcc gcagcgacat gatacactat cggttgtacg gcggcttcga cggcaccgaa
     3481 acggcgcgcg atgagcgcga ttgggccgcc catgagacca tcctgtcggg cgatatgagc
     3541 ggcgatgacg cgtgggatga cctgaacggc gcgtggacca accgggagga caatacctac
     3601 catgtcctgc tgttcgatcc gctcccgatc gaggatgctc tcaccataga cggcttcacc
     3661 gttaccggcg gcgaagcgac gggtgaggaa ggcgagcagc gtgacggagg aggtttttca
     3721 ctttcaaatc acacggttac gatccgcaac ctgaccttta tcggcaattc cgccgcggtg
     3781 aacggcaacg ccctctcctg caacggttgc gatgtcaccg tggaggatag ttatttctac
     3841 cgcaatatct tccccgatgg cacgaccggc ggcggcaccg tttccgtttc cgggacgctt
     3901 tcgatagccc gtgtcacctt tgaagagaac cgcggtcccg ttggcgccgt gtcttttaac
     3961 gccccgaccg gcggaacgct gatcgttgcc gacaccgatt tcttagacaa cagcgcttcg
     4021 gtcggcacgg ccgcgatatc gggcgccggc ggcgcggcaa tacttaccgg ttgtgctttc
     4081 atcggcaatg tcggttatgc ggccggcgcg gtggccctta ataatgtgga atcggcgatg
     4141 ctcgatgcct gctatttcga gaacaatgcg gcctcttcga tcgagccggg caatgaaggc
     4201 tccgccggcg ccctcagcgt gagcgaaggt tcgttctcca tcaccgattc gcagttcata
     4261 gggaacaccg cgtaccgtaa tgcggcgata tcgctttaca atatcggtaa cgcgacggtg
     4321 ctcggcgttg atttcatcga caacattggt tatacgaatg gcgctctcgg ggcgcccact
     4381 accgttctga cggtgggcgg ctgtgttttc tccgggaacg aggcgacatc gaatgaagag
     4441 ggtaaatggg gtaacggtgg cgcgatatcc tgcggggagg gcgccctgac cgtgaccaat
     4501 tcgaccttct ccggcaacag cgcctttacc ggcggcgtga tacaggccca caatgcggcg
     4561 ctttcgatat cgaattcctc gttcgacgcg aatgtgaccc acgctcccga tcagggaggt
     4621 aatgtttctg cggatgcagg aacggcggaa cttttgatag agggctcctc cttcacgaac
     4681 aacctcggcg cggcggtcac cgcgggcggc ggtcaactcg ttcagatatt cggctccctc
     4741 ttttccggga atcacgcggt caactcggcc gcgatcgcgg tgcagagcgg cagcagcgcc
     4801 atcatcgccg attgtcgttt catcgacaac gcggcggatg ctcccggcga aggcttcgcg
     4861 ggactcggcg gcgcgataat ggtcaacggg atggacgacg agaccggcgg cccggtagag
     4921 ggcgtcgata tctggatcgc caactcggtc tttgccggca atcgcgccaa tctgtggggc
     4981 ggcgcgatat tctcgctcct gctccatccg gtcattacca gttgtacctt ccatgataat
     5041 ctcgacagcg gtaacgatgc catcgtgttc gtcatgagcg atgctgtcat cgacaacagc
     5101 gtcatttggg acaagatgtc ggtccttgag ggcgaggtcg gtcccttcaa tttcggaccg
     5161 agtaatccga ccttcagtta cagcgatgta cggtatagcg gcggcagtgg caacgactgc
     5221 ggcggcggta gcgacccctg ctgggtcctg ccgttcggca ccgacggcgg cggcaatatc
     5281 gacgccgatc cgttgttcgt cggctcgggc gacgacccgt tcgacctgca aggcacttcg
     5341 ccgtgcattg acgccggaag caacgcgctg gtgccggaag gcattgattt cgacatcctc
     5401 ggcgatgccc ggatacagaa caccgcggtc gatatggggg cgtatgagca gtaggggcgt
     5461 gagtcgtgaa tcgtgaatgg cgagaagaaa aaccgaattg taggggcggg ttcgcccggc
     5521 aacaccctcc gtcgcttcgc gccacctccc tgcgacaggg aggcgatata cggaaggccc
     5581 cctgtcgcag ggggctccgc aacgcggcgg gggtgttaat tgtgtagggc gcaattcata
     5641 gcgccctgtt gaaagtgtag ggagttccca atgctcgatg tcgcgctgat catgccgttg
     5701 ttgaaaaagg agcgacggat actggcggcc taccttctgg ggagcgccgt gaatggtacc
     5761 ctgcgccccg acagcgacat cgacatcgcc ttgatgttcc tgccgggaaa aggcatgacg
     5821 ggcatcgaac ggggtgaact tgccgccgaa ctttcgctca tcctgcgacg cgaggtcgat
     5881 ctgggcgtca tttcttccgg caatctggtc tatgcccgcg aggcgatcct gaccggacga
     5941 cgcatattct cgcgtaacga tggcatcgcc gatctgtatg cggcgaccct gctgggactc
     6001 tacgcgcaat tccactatga acgacgggag atccttcatg gctatcttgc ccgatgatgt
     6061 gatacttaac aaggccgccg tcattgaacg ggcgctccgc cgtatgcggg aagagtacgc
     6121 attcgatccg gaactgaaga gttttaccca catcgatgcc atgacgctca acatcgagcg
     6181 ggcggcgcag gcggccatcg atctggctat gcatctggtg tcgcgagacc ggctgggggc
     6241 gccgcagacg agtggcgacg cgttccgcct gcttgccgaa gccggtgttg tatcgccgga
     6301 aacgcttcgc gccatgaccg ccatgatcgc attccgcaat atcgccgttc acgaatatca
     6361 ggaaatggat ctgtcggtcc tgcggaatat cgccggggat cggtggcgat cgctcgtcga
     6421 ttactgcgcc gagttgggaa taacgataac cgtagatcgt gggaagtgag tcgtgagtcg
     6481 tgaattgtgt gtttgtaggg gcgaaccggg gtgttaattg tgtaggggcg caatttatag
     6541 cgccctcttg aaatgacgag gaccgtatgt gcatagatgg atcgaaacca cacttttttc
     6601 ggaggttgac catgtggctt ctcaggaact ttttgacgct tgtcttcatg ttttcgctcg
     6661 ttttcctcgc cgcctgtgac ggcacgaaga cgacccccgg caccgatgcg ctcaccgacg
     6721 gtatcgcggc cgatgacgat ctcatcaccg acgacctcat ccccgatgaa gaggagccgg
     6781 atg
//
LOCUS       DAOQUE010000098         6765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12408_length_6765_cov_4.158569, whole genome shotgun sequence.
ACCESSION   DAOQUE010000098 DAOQUE010000000
VERSION     DAOQUE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12408_length_6765_cov_4.158569"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PKH10_04290"
     CDS             complement(<1..195)
                     /locus_tag="PKH10_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HNT27381.1"
                     /translation="MRMKDIDAIMKALPHRYPFLLVDRVLELEPEKKIRTLKNVTANE
                     PHFQGHFPFYPIMPGVLIVEA"
     gene            complement(192..1025)
                     /locus_tag="PKH10_04295"
     CDS             complement(192..1025)
                     /locus_tag="PKH10_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNT27382.1"
                     /translation="MLLKGKPAADAIIAGLASRGLPKLRLAVFHPAGDASAESYLKAK
                     EKALARLDFALDVVTVGRDTGADEFFALLDKANRDPGIHGIMVELPLPGGIPAHEVNR
                     RIDPAKDVDGVSYHNQGLLYATREEKLVPCTALGAVLLIEHYRIPVAGRRALIVGRSN
                     IVGLPLFKLLLNRDATVTVAHSRTPHLAKLVGGHDIVAVATGRPGLVRSVDVADGAAV
                     VDIGINVGPDGCLCGDFAPKDEKESERIHYSPVPGGAGVVTNAVMLRNLAECYHIQQG
                     R"
     gene            1161..2045
                     /locus_tag="PKH10_04300"
     CDS             1161..2045
                     /locus_tag="PKH10_04300"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNT27383.1"
                     /translation="MDPRFFQPLWDLMGIRGWNSYTVFEFLTLFLAYPLVYLVTRHIV
                     IERWKMFLNLSLMFYAGICGSKLFHILFDEQLSRFIGVIRNAGFLAFLRQEFLYPWKG
                     GQVFYGGILTAMAAGTLFGWFLFRKDRKLNILRTYDIIVFVFYEISAVGRIGCFLQGC
                     CYGAISETFGLVFPGRSAAASGLYRDGLLESSALATPPLIPTQLIEVCTSGSILIFLL
                     VKLPKAKQLWTGYYAWHALMFHAIARFCIEFLRIDRRGGLWLFSTSQWIAITVATVLI
                     IIRIRMLRKQRTIEASHV"
     gene            2038..4101
                     /gene="glyS"
                     /locus_tag="PKH10_04305"
     CDS             2038..4101
                     /gene="glyS"
                     /locus_tag="PKH10_04305"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNT27384.1"
                     /translation="MSDLLFEIGFEEFPPSFIRPAAEHLRSQMVEKMTAARIAPASVE
                     VFSTCSRIALKLGGFPAVQPELREKVQGAPKKVALGADGKLGKAGEAFLKKYNLTEYT
                     FEQSADGKGELITGWHVEPGRPVNDIIVETLEGLLSAMPFPKSMRWADGDLQFARPIR
                     WILCLIDDKPVERSFHGIAYGKSSFGHRFMAPGAVPVTFGTYVAALEKASVIVEHEKR
                     ALFIRAEVQRIARDLKGNDGVDEELVEEVANMTERPVAIVGTIPAKNADLPPELITLV
                     LKENQRYFTIYRSDRHELLPQFISFLNNAPTDPKVVVKGCEKVVAARLADAAFYYYDD
                     RKKDFAGLTEKLSGMMFQKELGSYRDKVERTAKLARFIGERYFGLDAKEAEAAAQAAL
                     LIKNDLITGVVFEFPELQGTMGRYYALNAGLDAVTAQALEEHYLPRFSGDRLPETVRG
                     AVLALADKTDTMVGGFMAGMKPTGAKDKFAIRRNAIGLLQIAAERGIALDIEELFAHA
                     AGLVTAHSAKLKLDAADLADFMKQRYYAIFPYETPVVKAAVEAGWRVPAEVKRRVATI
                     AKLVEEKDILEMVQLLKRSGNILKNAKDDEGRLIDEKKLTAAEEKELYAALAKVKKEV
                     EKTKDDLTAAHAIISMKPLIDRFFDKVLVMDEDPAVRGNRIALIRDIVTLVTARIGDI
                     SWLSM"
     gene            4116..4472
                     /locus_tag="PKH10_04310"
     CDS             4116..4472
                     /locus_tag="PKH10_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27385.1"
                     /translation="MATDARNIGKTIGKISVAISACAFLLGAGFAYWAGMTGDAKSAG
                     CGDPVSAIAIERYVLISSIISTATGALSYFLLAKQKIILRLFLTFVLGVVGFALLFFI
                     FLNFEDWGILYCYRHQ"
     gene            4520..5125
                     /locus_tag="PKH10_04315"
     CDS             4520..5125
                     /locus_tag="PKH10_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27386.1"
                     /translation="MTGNLRTTIILIALAVLGWYFWPSDGKEHPAGILAPDVPVQEDL
                     KAQPVIDHGEYTITGLAKFSLKARVLSAEPYWIDRGSGLVPVDLALGWGKMSDSAVID
                     QLDITQSVRFYRWNTKGSDYPIPKEEIISSSANMHLIPATKAIERRIKEAEKGDIVEF
                     SGYLARADRKDGFNWSSSMTRTDTGDGACEVVYVEHFRILP"
     gene            complement(5165..5959)
                     /locus_tag="PKH10_04320"
     CDS             complement(5165..5959)
                     /locus_tag="PKH10_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27387.1"
                     /translation="MKKVTKIMVLCGLMLVSLAFALVGCGGEDEGDKPGKILFKMVNP
                     GADAETTLAVRMLATSNPPLTGETTETILVGTKAGVGDIWVSQGIVKAGQPDDLEWVR
                     LTDTTNTELKSFEDYSLSPKEVPAGTYRSIKISLRNIFYYQTELASDRAVKYEILQTM
                     GSSEDPCDENDESWVKTNYFSDDGNHKLDENDKFEVVAPGEKVGGFTVEPGKTAVVSW
                     RWLMYGCKIFLIDKNGNLAFDCGVDDTEDECPPELKNMFDFVVEYQ"
     gene            6116..>6765
                     /locus_tag="PKH10_04325"
     CDS             6116..>6765
                     /locus_tag="PKH10_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27388.1"
                     /translation="MSIRRLFFVTASVLLFLAGCDNATTIPRDAAPGDHDTTAAVDED
                     DATGDDPLQNDDTGDGEPQSDDASPVERDDIDPGDHDLLSPDGEDGATGDDDAVGPDQ
                     ADDTIDDTIDDTTDDATDDASDDATDGTDTPLTDDGTTDDADTPLTDDGTSGDNDTPM
                     IDDTTMPDADVDTCVCSTGDCCDGCHFLSGTLCRPAVDLECDIPETCAGNDPDCPE"
BASE COUNT         1326 a         2170 c         1983 g         1286 t
ORIGIN      
        1 cgcctcgacg atgagcacgc ccggcatgat gggatagaac gggaaatggc cctgaaagtg
       61 cggctcgttg gccgtcacat tcttgagcgt ccgtatcttc ttctccggct caagttcgag
      121 cacgcggtcc acgagcagga acgggtaccg gtgcggcaac gccttcatga tcgcatcgat
      181 gtccttcatc ctcatctccc ctgttgtata tggtaacatt cggcaagatt cctcagcatc
      241 acggcgttgg tgaccacccc ggcgcccccg ggcaccggcg aatagtggat gcgctccgac
      301 tccttctcgt ccttcggcgc gaagtcaccg cacagacatc cgtcgggacc gacattgatg
      361 ccgatgtcca cgaccgccgc gccgtcggcg acatccaccg agcgtacgag ccccggacgc
      421 cccgtcgcca ccgcgacgat atcgtgcccg ccgaccagtt tcgcgaggtg cggcgtccgg
      481 ctgtgggcca ccgtgacggt cgcgtcgcgg ttcaggagca gtttgaagag cggcaggccg
      541 acgatgttcg accgcccgac gatgagcgcg cggcggcccg ccaccggtat ccggtaatgt
      601 tcgatcagaa gcaccgcccc gagcgccgta cacgggacga gtttttcctc gcgggtagca
      661 tagagcaacc cctgattgtg ataggatacg ccgtccacat cctttgccgg gtctatccgt
      721 cggttcacct catgagccgg tatgccgccg ggaagcggca gttcgaccat gatgccgtgg
      781 atgccggggt cgcggttcgc cttgtcgaga agcgcgaaga actcgtcggc gccggtatcc
      841 ctgcccaccg tgacgacatc gagcgcgaag tcgagccgcg cgagcgcctt ctccttcgct
      901 ttgaggtaac tttcggcgga cgcgtcgccc gcggggtgga acaccgcgag tctcagtttc
      961 ggcaggccgc gggatgccag gccggcgatg atcgcgtcgg ccgccggttt ccccttcaga
     1021 agcatcgctc acctcatcgg catggataat gaacgggacc gagtataacg cggcggcggc
     1081 cgattgcaag agaaagaccg ctccgcttga cttcaccgcc cgaacaccta tcatcggggg
     1141 gacaacaacg cgaggcgacg atggatccga gattctttca gccgctgtgg gacctcatgg
     1201 gcatacgggg gtggaattcc tacaccgtgt tcgagttcct gacgcttttc ctcgcctatc
     1261 cgctcgtcta tctcgtcacg cgccacatcg tcatagaacg gtggaagatg ttcctcaacc
     1321 tctcgttgat gttctatgcg ggcatctgcg gttcgaaact gttccacatc ctgttcgacg
     1381 aacaactctc ccgcttcatc ggggtcatcc gcaacgccgg gttcctcgca ttcctgcggc
     1441 aggagttcct ctatccctgg aaaggcgggc aggtcttcta cggcggcatc ctcaccgcga
     1501 tggcggccgg taccctgttc ggctggttcc tgttccggaa ggaccggaaa ctcaacatcc
     1561 tccgcaccta cgacatcatc gtgttcgtct tctacgagat atcggcggtc ggccggatcg
     1621 gctgtttcct gcaggggtgc tgttacggcg cgataagcga gaccttcggg ctcgtcttcc
     1681 ccggtcgttc ggccgccgcg agcgggctct accgggacgg gcttctcgag tcctccgcgc
     1741 tggcgacccc gccgctgata ccgacccaac tcatcgaggt ctgcacgagc ggctcgatac
     1801 tgatattcct gctcgtaaaa ctcccgaagg ccaaacagtt gtggaccggc tattacgcgt
     1861 ggcacgcgct gatgttccat gccatcgcgc ggttctgcat cgagttcctg cgcatcgacc
     1921 ggcgtggcgg tctgtggctg ttctccactt cgcagtggat cgcgatcacc gtcgcgacgg
     1981 tactcatcat cattcgcatc cgtatgctga gaaaacaacg caccatagag gcttcccatg
     2041 tctgacctgc tcttcgagat cggtttcgag gaattccccc cctccttcat ccgtccggcg
     2101 gccgaacact tgcggtcgca gatggtcgag aagatgaccg cggcgcgcat cgccccggcg
     2161 tcggttgagg tgttcagcac ctgttcccgc atcgccctga aactgggcgg ctttccggcg
     2221 gtccagcccg aactgcgcga gaaggtgcag ggggcgccca agaaggtggc gctcggcgcc
     2281 gacggcaaac tgggcaaggc gggcgaggcg ttcctcaaga aatataacct taccgaatac
     2341 acctttgaac agtcggccga cggcaaaggg gaactcatca ccggctggca tgtcgagccg
     2401 ggccgccccg tcaacgacat catcgtcgag acgctggagg gacttctttc agcgatgccg
     2461 ttcccgaaat cgatgcgctg ggccgatggc gacctgcagt tcgcgcgacc gatacgctgg
     2521 atcctctgcc tcatcgacga caagccggtg gaacggtcgt tccacggcat cgcctacggg
     2581 aagagctcgt tcggtcaccg cttcatggcc cccggcgcgg taccggtcac cttcggcacc
     2641 tatgtcgccg cgctcgaaaa ggcctccgtc atcgtcgaac acgagaaacg ggcccttttc
     2701 atccgcgccg aggtccagcg gatcgcccgc gacctcaaag ggaacgacgg cgtcgacgag
     2761 gaactggtcg aagaggtcgc caacatgacc gaacggccgg tggcgatcgt cggcaccatc
     2821 ccggcgaaga acgccgatct gccgcccgaa ctgatcacgc tcgtccttaa agagaatcag
     2881 cgctatttca ccatctaccg gtccgaccgg cacgaactcc tgccgcagtt catatcgttc
     2941 ctcaacaacg ccccgaccga tcccaaagtg gtcgtgaagg ggtgtgaaaa ggtggtcgcg
     3001 gcgcgtcttg ccgacgcggc gttctactac tacgacgacc gcaagaagga cttcgcgggg
     3061 ctcaccgaaa aactttccgg catgatgttc cagaaggaac tcggctccta ccgcgacaag
     3121 gtggaacgga ccgctaaact ggcgcgcttc atcggcgagc ggtacttcgg actcgacgcg
     3181 aaagaggctg aggcggcggc gcaggcggcg ctcctcatca agaacgatct catcaccggc
     3241 gtcgtcttcg agttccccga actgcaaggg acgatggggc gttactacgc cctgaacgcc
     3301 ggactcgacg ccgtgacggc gcaggcgctc gaagaacact acctgccccg cttctcgggc
     3361 gaccggctcc ccgaaacggt gcgcggcgcg gtgctcgcgc tcgccgacaa gaccgacacg
     3421 atggtcggcg gcttcatggc ggggatgaaa ccgaccggcg cgaaggacaa gttcgcgata
     3481 cggcgcaacg ccatcggcct tctccagata gcggccgaac ggggcatcgc tctcgacatc
     3541 gaggaacttt tcgcccatgc cgcgggcctc gtgacggcgc acagcgcgaa actgaaactc
     3601 gatgcggccg acctcgccga cttcatgaaa cagcgctact acgcgatatt cccgtatgaa
     3661 acgccggtcg tgaaggctgc ggtcgaggcg ggctggcggg tgccggccga ggtgaaacgg
     3721 cgcgtcgcca ccatcgcgaa actggtcgag gaaaaagata tccttgagat ggtgcaactg
     3781 ctcaagcgct ccggcaacat cctcaagaac gcgaaggatg atgaggggcg cctcatcgac
     3841 gagaagaaac tgacggcggc cgaggagaag gagctgtatg cggcgctcgc gaaggtgaaa
     3901 aaagaggtcg aaaaaacgaa ggatgacctc acggcggcgc acgcgatcat atcgatgaaa
     3961 ccgctcatcg accgcttctt cgacaaggtg ctcgtcatgg acgaagaccc cgccgtccgg
     4021 ggcaatcgca tcgcgctcat ccgcgacatc gtcactttgg tcaccgcccg catcggcgac
     4081 atctcgtggc tcagcatgta gcagggggtt attgaatggc gacggacgca agaaacatag
     4141 ggaaaacaat cggtaagata tccgtcgcaa tatcggcctg cgcgttcctg ctgggcgctg
     4201 gctttgcgta ctgggcaggg atgacagggg acgcaaagtc ggccggttgt ggcgaccccg
     4261 tttctgcgat agcgattgag cgttatgtcc ttatatcttc aataatttca acagcaaccg
     4321 gagctctctc atatttcctg cttgcaaagc aaaaaatcat tcttcgcttg tttcttactt
     4381 tcgtattggg cgtggtcggc tttgcattgt tgttctttat atttcttaat ttcgaagatt
     4441 gggggatact ttattgttac cgtcatcaat gatcatccgt cctgaaacac ctgccgacta
     4501 tccggcgata cgataaccga tgaccggtaa cctgcgcact accattatat taatagcgct
     4561 cgcggttctc ggctggtact tctggccatc ggacggcaag gaacaccccg ccgggatact
     4621 cgcccccgat gtgccggtgc aggaggatct caaggcccag ccggtcatcg accacggcga
     4681 atacaccatc accgggctcg ccaagttctc gctgaaggcg cgcgtcctgt cggccgaacc
     4741 ttactggata gaccgcggct cggggctcgt gccggtcgat ctcgcgctcg gctggggaaa
     4801 aatgtctgac agcgcggtca tcgatcaact ggacatcacg cagtcggtca ggttctaccg
     4861 ctggaacacc aaagggagcg actaccccat cccgaaagag gagatcatct cgtcgagcgc
     4921 caacatgcac ctcatcccgg cgaccaaggc gatagagcgg cgcatcaagg aggcggagaa
     4981 gggcgacatc gtcgagttct ccggctacct cgcgcgggcc gaccggaagg acggcttcaa
     5041 ctggtcgagt tcgatgaccc gcaccgacac cggcgacggg gcgtgcgaag tggtctatgt
     5101 ggagcatttc aggatactgc cgtgaacccg atgaggaccc cgtgaccggg gcgcccgcct
     5161 gccgctactg atattccacg acgaaatcga acatgttctt caactcggga gggcactcgt
     5221 cctcggtatc gtcgacgccg cagtcgaacg ccagattccc gttcttgtct atcaggaaga
     5281 tcttgcatcc gtacatgagc cagcgccaac tcaccacggc ggtcttcccc ggctcgaccg
     5341 tgaacccgcc gaccttctcg ccgggcgcaa ccacctcgaa cttgtcgttc tcatccagtt
     5401 tgtggttccc gtcgtccgag aaatagttgg tcttcaccca actctcatcg ttctcgtcgc
     5461 agggatcctc ggaactgccc atcgtctgca ggatctcgta tttcaccgcg cggtcggagg
     5521 cgagttcggt ctggtagtaa aatatgttcc gcagcgatat cttgatgctc cggtaggtgc
     5581 ctgccgggac ctccttgggc gacagcgagt aatcctcgaa cgacttcagt tcggtattgg
     5641 tggtgtcggt gagccgcacc cattcgagat cgtcgggctg tcccgccttc acgatcccct
     5701 gcgacaccca gatatcgccg acccccgctt tcgtgccgac caatatggtc tcggtggtct
     5761 cgccggtaag cggcgggttc gatgtcgcca gcatccgcac cgcgagcgtc gtctcggcgt
     5821 ccgcgcccgg attgaccatc ttgaacagga tcttccccgg cttgtccccc tcgtcctcgc
     5881 cgccgcaccc gacaagagcg aacgcgagag acacgagcat gagcccgcac aacaccatga
     5941 tcttcgtgac ctttttcatc ttgccccctc cctgaacggt taatgaccaa cgcgccatga
     6001 taccggcttc cagaggtcga agcaagaaaa aacggatact gccctgattt tttgatattc
     6061 ccgcgcgtcc gtagtagtat ctttcaactt atggtgttat cggtggaggg tattcatgag
     6121 catacgccgt ctttttttcg tcaccgcgtc cgttctgctg ttccttgccg gatgcgataa
     6181 cgcgaccact atcccccgtg acgccgcgcc gggtgaccac gacacgaccg ccgccgtgga
     6241 cgaggatgac gcgacgggcg atgacccgct ccagaacgac gatacgggcg acggtgagcc
     6301 gcaaagcgac gacgcctccc cggtcgaacg cgacgacatc gaccccggcg accatgacct
     6361 cctctccccc gacggcgagg acggcgcgac gggcgacgac gatgcggtcg ggcccgatca
     6421 ggccgacgac acgatcgacg acacgatcga cgacacgacc gatgacgcga ccgatgacgc
     6481 ctccgatgac gcgaccgacg gcaccgatac tcccctcacc gacgatggca cgaccgatga
     6541 tgccgatacg ccactcaccg acgacggtac gagcggcgac aacgacacgc cgatgatcga
     6601 cgacacgacg atgcccgacg ccgatgtcga cacctgcgtc tgctcgacgg gggattgctg
     6661 cgacggctgt catttccttt cgggcacgct ctgccgcccg gcggtcgatc tcgagtgcga
     6721 catccccgag acctgcgcgg gcaacgaccc cgactgcccc gaaga
//
LOCUS       DAOQUE010000099         6763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12414_length_6763_cov_4.700805, whole genome shotgun sequence.
ACCESSION   DAOQUE010000099 DAOQUE010000000
VERSION     DAOQUE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12414_length_6763_cov_4.700805"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4330)
                     /locus_tag="PKH10_04330"
     CDS             complement(<1..4330)
                     /locus_tag="PKH10_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27389.1"
                     /translation="MKFMVSKNILTKIGTGGGNLLRTLLIITIFPATIFSSTQGAAGT
                     GLPMAVKMRKFVTEKMILDVAVSAIGSSRTFTVPVSSLPGDMREQLSGKADSIKIEAD
                     LEVSNVYFARNVSLETLSDPAKRNRVYVLVNIKRPTSRQFANFLDEYGQIITEIYTPF
                     GSVELGDFSDYFSAMLLSMKSIDRDSAKSNAITEFPQLYVVMSTSFANGVLTVTPERA
                     ELKDRSNTDTFSNAIETSITEAFVSLDPIQYDLTEKIPTTYLWEHYQNCLDACTNEYE
                     KCMYCTDAERRYCEGQTTDPVWIEWCLGVYCAEGDPVYCAEEHNVTECENECKDRSVH
                     MTAQSLVFKTGPFQTPASPDHLLEANTFGSEFTFTTQNGSVTVVSCPAVSPKVSDAVN
                     VFSILDEKGPRLKELLQLSLSEDILEEIFAALLNRTVNQYDVFGKADISIRVRVKDAK
                     VMHDTHGNYLEVTLGDKITSDPAFLCSGSFSGKIRLRVTHNSSGALVLRPDIPASGDF
                     EFDVSNQEIIELFVDLGSYTDKLTKFLVDIPLDTMLGSFNELINGFIGGQNISKDAWF
                     HRVAGSGDAPNVIGDLVIGWYGEYPANLLKNYSILMANPDKDGKLNIDDLCPDTPELK
                     DPKHRCIYDDLDGDGIRDIADPRLFHLSVKKQKQHKSESTENENKRTPGPGHCLNWTW
                     QQNDTYGRTVEIAGAVTTRDIYAVANAKSYQSHYYCYCGQGGNKAKCPAETGDCGAGH
                     AAPKVAWQSLGGVQKTTWRAIHAQKENAPRTVKTTTQTAPYNRSWQWTTDMAEYKPYG
                     CADTEFKESRAAYNQAHDAGCYFNARLSAGFVRSADNAGYMSGQSINEPFFEGYFVTN
                     PAYPAFQHTALSLMTDKGRWTTSPDWNDKPLELIYWQTGWEDRQIPMCNVSPSLDYDA
                     LNQRYRDLIGKGRFNPDNIDPVDSLGWLKARDIFGIDRIGDKIVVEPEQPPVGWQSLD
                     KIGGMTYAFVKQGSAYALSVGSAALPLGAPKQVSGGRDHNFAALALFGNILYLAGNYV
                     AVVSPPPPGDLTPEFGATFHFENVLGRLIEHDDYWEYQSLVSFPANYQIENLSLAILN
                     DSLVAVVRATDGTLYLYRHDTDTNEWITEGSMTIPATPLFSSVKVVGEALYFAAHESN
                     ATTLYRYTTDAGVTLFATLDPAVLRNGIRFFGDAAGLLTAVAVENLESGTASAFTVAT
                     DGTVVPSLFDVDQQINLPDLFCLLEEGALLKGGTGNNGVCLPFTSPWYKSFSVGTTVY
                     SVAGKGDRLYVGTNSTIRVYDISDPNALVLKSTFTTNRRVYDLEVAEGDIMYAATSGG
                     IYKFNTANPDAISQLSFYSTPYNYQYRIQLYNDKLYVGDDNGINIRDKETFARLAYVN
                     LGSVVDFAITNGELALYWYAFWDEGVQVRDADTLTLKAYEYGYCSTGELTTDHGAFYL
                     ACDGYEY"
     gene            complement(4432..6045)
                     /locus_tag="PKH10_04335"
     CDS             complement(4432..6045)
                     /locus_tag="PKH10_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27390.1"
                     /translation="MEHFQFLAFPEQLPSVVKKQGHIPVVPLWFPGEDLDRIVPWLRR
                     REHPFALAFDRDRASVVPLARALTVADRLFLFSPDDAERARAARFAGALFLFSSGAML
                     SWPQWYRDHKVRPVVSGRPNWEAARGLDPLYLATTKRCIAEFGTCRARDGRIQQFTAC
                     DLRCRDKVRERQSVTAPFSLRPEYTHPPDAKALVAYDESVAPPVPRPAPLAEPVLNDA
                     WNYPWPLGRRILREAHKTGDTRFHVILHAEERRLYEDGLVAFWCYDRTMYDGEWLEPS
                     RATVTSGRLLLTFPRPVRKALFVQRYDEATKDLLKRARHQIYTMPSGRLFFTTPEKPK
                     GAEDLRPLRTPARRGSTRLTVVLDDPDRAAFFRIRDIDRVALLHDGRPVGKFFNHHLY
                     IPFPLKEEEARQIAAHPRLRGVIVRDRLMQKMFESLFEGRREVLLHPATPRAESEGAA
                     PSYLAGAEQVFSSIYTAEHRKDYHWRSLGLRTANRRGHTLFLRDTKVLQEGSLAELWV
                     DIIGADDATVRDLKMRAERYLKEKRVTVA"
     gene            complement(6051..6311)
                     /locus_tag="PKH10_04340"
     CDS             complement(6051..6311)
                     /locus_tag="PKH10_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HNT27391.1"
                     /translation="MSTEKRADLNLKIARLPIVDQCEGCQRIVEDAGAKVCGKYAEPA
                     FHWEDGQICLMATHINIKKEAAVAKKAVNPLKASKRAAGKKK"
     gene            6454..>6763
                     /locus_tag="PKH10_04345"
     CDS             6454..>6763
                     /locus_tag="PKH10_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015635977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNT27392.1"
                     /translation="MSAQRKVSPGTTVVHGIHGHHATTHDLVPPIHLTSTFVFKNADH
                     GAGCFAGTDEGYIYTRLGNPTLDLLGQKLAAIEGAEMGLTAASGMAAIAAVAMSFCRP
                     G"
BASE COUNT         1445 a         1851 c         1960 g         1507 t
ORIGIN      
        1 gatactcgta cccgtcgcag gcgagataga acgccccgtg gtcggtcgtc agttctccgg
       61 tggagcagta accgtactcg taggccttga gcgtgagcgt atcggcgtcg cgcacctgca
      121 ccccttcatc ccagaaggcg taccagtaga gggccagttc tccgttcgtg atggcgaagt
      181 cgaccaccga acccaagttc acataggcga gacgggcgaa ggtctccttg tcgcggatgt
      241 tgatgccgtt gtcgtcgccg acatagagtt tgtcgttgta gagttggatg cggtactggt
      301 agttgtacgg cgtgctgtag aacgacaact ggcttatcgc gtctgggttc gcggtgttga
      361 acttgtagat gccgccgctc gtcgccgcgt acatgatgtc gccctcggcc acttcgaggt
      421 cgtaaacgcg ccggttggtg gtgaaagtgc tcttgaggac cagcgcgttt gggtcgctga
      481 tatcgtaaac gcggatcgtg ctgttcgtcc cgacatagag ccggtcgccc ttcccggcta
      541 cgctgtagac ggtcgtgccg acggagaagg atttgtacca cggggaggtg aagggaaggc
      601 acacaccgtt gtttcccgtg ccgcccttca gaagagcgcc ctcttcgagc aggcagaaaa
      661 gatcggggag attgatctgc tgatcgacat caaagagaga tgggacaacc gtcccatccg
      721 ttgcgacggt aaatgctgaa gccgtcccgc tttcaagatt ctcaacggcc actgccgtca
      781 acagacccgc ggcatcgccg aaaaagcgga tcccgttgcg caggactgca ggatcgagtg
      841 tcgcgaaaag ggtcacgccg gcatcggtcg tgtagcggta cagagtcgtg gcattcgatt
      901 cgtgcgcggc aaagtaaagg gcctctccaa cgacttttac cgacgagaac agcggcgtgg
      961 ccggaatggt catcgagccc tccgtgatcc actcattggt gtcggtgtcg tgccggtaaa
     1021 ggtacagtgt tccatcggtg gcgcgcacta ccgctacgag agaatcgttc agtatcgcga
     1081 gcgaaagatt ctcgatctga tagttggcgg ggaaggagac aagggactga tactcccaat
     1141 aatcgtcgtg ttcgataagg cgaccgagta cattttcgaa atgaaatgtc gcgccgaatt
     1201 caggagttag atccccgggc ggcggcggcg agacaacggc gacatagtta ccggcgagat
     1261 agaggatgtt tccgaagagc gcaagcgcgg cgaagttatg atcacggcct cccgatacct
     1321 gcttgggcgc accgagcggt aatgcggcgg acccgaccga aagtgcgtaa gcgcttccct
     1381 gtttaacgaa ggcataggtc atgccaccta ttttatcgag cgattgccag ccgaccggcg
     1441 gttgttcggg ctctacgacg attttgtccc cgattctgtc gataccgaag atatcgcgcg
     1501 ctttcagcca ccccaaagaa tcgaccggat cgatattgtc ggggttaaag cgacctttgc
     1561 cgataagatc gcgatagcgt tgattgagcg cgtcgtaatc gagcgaaggg gagacattgc
     1621 acatgggaat ttgccgatct tcccatccgg tctgccaata gatgagttcc agcggtttgt
     1681 cgttccaatc gggagaggtg gtccaacgcc ccttgtcggt catcagcgaa agagccgtgt
     1741 gctggaaggc gggataggcg gggttcgtta cgaaataccc ttcgaagaac ggctcattga
     1801 tgctttggcc gctcatataa ccggcgttat cggcactgcg gacaaaaccg gcggaaaggc
     1861 gggcattgaa ataacagccc gcatcgtgtg cttgattgta tgccgcacgg gactctttga
     1921 attctgtatc ggcgcaaccg tagggcttat actcggccat gtcggtcgtc cactgccatg
     1981 agcggttgta gggggctgtt tgtgtcgtcg ttttaaccgt gcggggtgcg ttttctttct
     2041 gggcgtgaat ggcgcgccaa gtggtttttt gaacaccacc taaagattgc catgcaacct
     2101 tcggcgccgc gtgtcccgcg ccacagtcac ccgtttcggc gggacatttt gccttattcc
     2161 ccccctgacc acagtagcag tagtaatgcg attggtaact tttcgcattg gccaccgcgt
     2221 atatatcgcg ggtcgtgacg gcacccgcta tctctaccgt tcggccatag gtgtcattct
     2281 gttgccaagt ccaattcaaa cagtgtccgg gccccggcgt ccgtttgttt tcgttttccg
     2341 tcgattcgct tttgtgctgt ttctgttttt tcaccgaaag atggaaaagc cgcggatcgg
     2401 cgatgtcgcg gattccgtcg ccgtcgaggt cgtcatagat gcatcgatgt ttcgggtctt
     2461 ttaattccgg cgtatcggga cagagatcat cgatgttgag ctttccatct ttgtcgggat
     2521 tggccataag gatcgagtaa ttttttagaa gattggcggg atattcgcca taccaaccaa
     2581 tgacgaggtc gccaatcaca ttcggggcat caccgcttcc tgcgacacgg tggaaccatg
     2641 cgtcttttga aatattttgt ccgcctataa aaccattgat caattcgtta aatgaaccca
     2701 acatcgtatc aagaggaatg tcaaccaaga atttagtcag tttgtcggta taagaaccca
     2761 aatcgacgaa caattctatt atctcttgat tcgacacatc aaattcaaaa tcgccggaag
     2821 ccggtatatc gggacgcaaa accagcgcgc cggaactatt gtgcgtaacg cgcagtctga
     2881 tttttccact aaacgatcca ctgcataaaa atgccggatc ggatgttatt ttgtcgccca
     2941 aggtgacttc caaataattc ccgtgggtat cgtgcatgac cttcgcatct tttacacgaa
     3001 cgcgaatgct gatatcggcc ttcccgaaca catcgtattg attgactgtg cggtttaaga
     3061 gagcggcgaa tatctcttca agaatatcct cgctcaatga aagttgaagc aactccttta
     3121 gcctcggacc cttttcatct aagatgctaa aaacattaac cgcatccgat actttcggtg
     3181 acaccgccgg gcaacttacc accgttacgg aaccgttttg ggttgtaaag gtgaattcac
     3241 taccaaaggt gttggcttcc aataggtgat caggcgaggc aggtgtttga aagggacccg
     3301 tcttgaaaac aagactctga gcagtcatgt gaactgaacg atctttacat tcgttctcac
     3361 actcggtcac attgtgttct tctgcgcagt agacagggtc accctcggca cagtatacgc
     3421 ccaaacacca ttcgatccaa acggggtccg tagtttgacc ttcgcaataa cgccgttcgg
     3481 catcggtgca gtacatacac ttctcatatt cgttggtaca ggcatcgagg caattttggt
     3541 aatgctccca aagataggtt gtcggaatct tttcggtcag atcgtactgg atcggatcca
     3601 aggagacgaa tgcctcggtg atgcttgttt caatagcgtt cgaaaaagta tcggtattgc
     3661 ttcgatcctt cagttcggcc ctttccggcg ttaccgtcaa aaccccgttt gcgaaagagg
     3721 tgctcatgac aacatacaat tgaggaaatt ccgttatcgc gttactcttg gcactatccc
     3781 ggtcgatcga cttcatcgaa agtaacatgg cggagaaata atcgctgaag tcaccaagtt
     3841 caacagagcc aaacggtgta tagatctcgg taattatttg cccatattcg tcgagaaagt
     3901 tcgcaaactg acgactcgtc gggcgtttga tgttcaccaa cacatagact cgattccgtt
     3961 tggccggatc agaaagtgtt tcaagactta cattccgcgc aaaatagaca ttggacactt
     4021 cgagatcggc ctctatcttt atactatcgg ctttcccgga gagttgttcc ctcatatctc
     4081 ctggaagcga cgagacggga acagtaaaag ttcgcgaaga cccgatagca gaaaccgcca
     4141 catcgagaat cattttctcg gtaacaaatt tacgcatctt caccgccatg ggcaaaccgg
     4201 taccagcggc accctgcgtg gacgaaaaaa ttgttgcggg aaaaatcgtg ataattagca
     4261 gtgttcttaa gaggttaccc ccccccgtgc cgattttagt taaaatgttt tttgaaacca
     4321 tgaacttcat aacacacctc cccagatgaa tgaatagaca actcgacttt tacaaaatga
     4381 gattatcggt gtcaagattt ttttaggaca gtattgttgt aaagatgggg gttacgcgac
     4441 cgttacccgt ttttctttca ggtaccgttc ggcgcgcatc ttgaggtcgc gcaccgtcgc
     4501 gtcgtcggcg ccgatgatgt cgacccacag ttcggcaagg gaaccttctt gtaacacctt
     4561 ggtatccctc aggaacaagg tgtggccgcg ccggttcgcc gtccgcaggc cgagcgaccg
     4621 ccagtggtag tctttccggt gctctgcggt gtagatcgag gagaacacct gttccgctcc
     4681 cgcaaggtag gagggtgccg cgccttcgct ttccgcccgg ggcgtcgccg ggtgcaggag
     4741 cacctcccgt cgcccttcga agagcgactc gaacatcttt tgcatgagcc ggtcccgcac
     4801 gatgacgccg cgcaggcggg ggtgggccgc gatctgtcgc gcctcctctt ccttcaacgg
     4861 gaacgggata tacaggtggt ggttgaagaa cttgccgacc ggtcgcccgt catggaggag
     4921 cgcgacgcga tcgatgtcgc ggatacggaa gaatgctgcg cggtcgggat cgtcgagcac
     4981 gacggtcaac cgggtcgatc cgcggcgcgc cggggtacgg agcgggcgga ggtcttccgc
     5041 tcctttcggt ttttcgggtg tggtgaagaa cagccgcccc gaaggcatgg tatatatctg
     5101 gtggcgcgcc cgcttcagca gatctttggt cgcctcgtcg tagcgttgca cgaagagcgc
     5161 cttccgtacc gggcggggaa aagtgaggag cagccgtccg ctcgtcacgg tcgcccgcga
     5221 cggctccagc cactcgccgt cgtacatcgt ccggtcgtaa caccagaagg cgacgaggcc
     5281 gtcttcgtac agacgccgtt cctcggcgtg caggatgaca tggaaccggg tgtcgcccgt
     5341 cttgtgcgcc tcccggagga tgcggcggcc gagaggccac gggtagttcc acgcgtcgtt
     5401 gagcaccggc tcggcgagcg gcgcgggacg cgggacgggc ggcgcgaccg actcgtcgta
     5461 agcgacgagc gccttggcgt cgggcggatg ggtgtattcg gggcgcaatg agaacggcgc
     5521 ggtgaccgac tgccgttcgc gcaccttgtc gcggcaccgg agatcgcagg cggtgaactg
     5581 ctgtatgcga ccgtcgcggg cgcggcaggt gccgaactcg gcgatacagc gcttggtcgt
     5641 ggcgagatag agcggatcaa ggccacgagc cgcctcccag ttcgggcgcc ccgatacgac
     5701 cggccgcacc ttgtggtcgc ggtaccactg cggccacgag agcatcgcgc cgctggaaaa
     5761 aaggaacagt gcgccggcaa aacgggcggc tcgcgcccgc tcggcatcat ccggagagaa
     5821 caggaacagc cggtcggcga ccgtcagcgc ccgtgccaag gggacgaccg acgcacggtc
     5881 gcggtcgaag gcgagcgcga aaggatgttc gcggcgccgg agccacggga cgatgcggtc
     5941 cagatcctct ccggggaacc aaagcggcac gaccgggata tggccttgct tttttacgac
     6001 ggagggaagt tgttcgggaa aggcgagaaa ctggaagtgt tccaacggga ctacttcttc
     6061 tttccggccg cccgtttaga ggccttgagc gggttgaccg ccttcttggc gaccgccgct
     6121 tccttcttga tattgatatg ggtcgccatg aggcatatct gaccatcctc ccagtggaag
     6181 gccggctcag cgtacttgcc gcagaccttg gcgcccgcat cttccacgat gcgctggcag
     6241 ccttcgcact ggtccactat cggcagacgg gcgatcttga ggttgaggtc ggcgcgtttc
     6301 tcggtgctca tcgttatcct ccacgactta tcacacagcg gcgcgattaa acagaaaaag
     6361 gggcgattgt caataaaatg tttctttcgg aagaacttgc cctgcggcga aaagcggtta
     6421 gcgtaccggc ggtcactacc gtatggaggc accatgtcgg cacaacggaa agtttctccc
     6481 gggaccacgg tggtccacgg gatccacggc catcacgcga cgacccatga tctcgtcccg
     6541 cccatccacc tcacctcgac cttcgtcttc aagaacgccg accacggcgc cggctgtttc
     6601 gccgggaccg acgaagggta catctacacg cggctcggca acccgacgct cgacctgctc
     6661 ggccagaaac tcgcggcgat cgaaggggcc gagatggggc tcaccgccgc cagcggcatg
     6721 gcggccatcg cggcggtcgc gatgagtttc tgccgccccg gcg
//
LOCUS       DAOQUE010000100         6654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12674_length_6654_cov_4.587665, whole genome shotgun sequence.
ACCESSION   DAOQUE010000100 DAOQUE010000000
VERSION     DAOQUE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12674_length_6654_cov_4.587665"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PKH10_04350"
     CDS             complement(<1..746)
                     /locus_tag="PKH10_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT27393.1"
                     /translation="MRTRDLPLDITAGPVRATLLALAVPVIILNILKAGYNIVDMFWI
                     GRLGPDYLAGVSGSVFLVWAIHGLSHLITTGIAAGVSRNAGEGRDDRAAANAGRALFY
                     TVGAGAILTVLIYPIIPDLIAYVGMSPVATTAGVQYLEVMTMFTVVIFLLFSLHSIMI
                     ARGDTVMPVRVYGATFVLNILLSPVLMFGMGPFPRLETRGAAYATIVSYLVACVWFWL
                     LMRRRGWVSFRGAGPDGLIPLRRYLSLGYPV"
     gene            919..2403
                     /locus_tag="PKH10_04355"
     CDS             919..2403
                     /locus_tag="PKH10_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27394.1"
                     /translation="MKRIVCSCAVLAAIVLCTPALDAAKKKGKAKTKKPGTALVTGPK
                     KCDELEAEFKRKVNDLKAKWKLSDEGLDRSLDETERKLTDMDRNGAAELRRYAAELEG
                     QKKQELDAAKRDHEKAMAQFEKKDDGAKRSFDMEAERHRRKIADLERRKDESQKEMQR
                     IESVRDRLETERSIEEQRLLAEYEKVKLAAEDESRKYRKQVEDEYQKKFDELNAKLGE
                     WDAKEAEKRKETEGFDRQIEEFAMQTAQKLDAFQQEIEKKRAEAQAKFKDEELEKAEM
                     ELDMALAQESQRLNAEIERYRKERLEKKKEKEQQVKEFLATKDDFRKRNDRLVQMAQQ
                     YRDSQLEKRKAEEMKAQERWNNEKKRRDERYERRLQMEYREKRDALVKKQDQLSLEIE
                     QEKSGLVTLEGKFRQQTDSKEAAIESEKGRITERYKKTVAKIQDKYDMMEVEEKHTQE
                     LVRDQLEEKRDSLIATRNKNKADRQELLYKMKDDYEEQKGSCVK"
     gene            2525..2791
                     /gene="rpsO"
                     /locus_tag="PKH10_04360"
     CDS             2525..2791
                     /gene="rpsO"
                     /locus_tag="PKH10_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNT27395.1"
                     /translation="MLERVAKQGVITKFKTHEQDTGSPEVQIALLTTRINQLTEHFKF
                     HKKDNHGRLGLLKMVGKRAKLLRYLKDNHFTRYSDLIKELGLRK"
     gene            2849..5110
                     /gene="pnp"
                     /locus_tag="PKH10_04365"
     CDS             2849..5110
                     /gene="pnp"
                     /locus_tag="PKH10_04365"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNT27396.1"
                     /translation="MKTHHVESIVFEGTPLSLETGKYAKQADGAVWCRYGNSVLLATA
                     VAAKGDVAAEQDFFPLTVDYIEKFYAAGRFPGGFLKRESQPNTDEKLAARIIDRPIRP
                     LFPDWYRSETQLLVNLFSHDENCDPRVIAITAASAALMISDVPFLGPIGGVRVVLKDG
                     EYFINPTPQAQEGARLNIIMAANRDSILMVEGEAREATEDEIAEALARGHRATLPLIE
                     MQEALMAKVAPVKRQDPQNPDDAALRNFIELFAVDKLKEALAIREKLARYEAIKEVKT
                     SVGERVAESLAGGAQFLNADEPEKVQNRAKAIFEDVLKKTMRRMITEEGRRIDGRGPT
                     NIRPIWIETGVLPQVHGSAIFTRGETQSLGTITLGVGSDEQRVDTVLEETRKTFMLHY
                     NFPPFSVGEVGRLRAPGRREIGHGNLAERALKSFVPPKEEFAYTVRVVSEILESNGSS
                     SMASVCSGCLALMDAGVPFKKHVAGIAMGLIKEGDDYAILSDIMGDEDHLGDMDFKVA
                     GTKDGITAIQMDIKIHGLPQEVMRRALAQARDGRIHIIGEMEKALPEHRAELSPLAPK
                     LVTIHINPERIRDLIGTGGKNIKSIVEKTGAEIEVQQDGSVLIAAKDGAVLDAAIAMV
                     KSFTDEPEKGKTYEGVVTRIEDYGAFVEVMPGQTGLLHVSRIADKRIESVSDVLKLGD
                     TVRVIVVELEGAGKFKLSMKPSDFERDFTKDDGEEPRRERRDRKPHGDRDRDRGRDRD
                     RDRKPHGDRKDRS"
     gene            5070..5549
                     /gene="dut"
                     /locus_tag="PKH10_04370"
     CDS             5070..5549
                     /gene="dut"
                     /locus_tag="PKH10_04370"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNT27397.1"
                     /translation="MTVNPTATGKTAHNMTDGVTVKITAAPGLPLPSYQTAGSSGADV
                     RASTDTIIPPRGWAAVPTGLFCEIPSGWEIQVRSRSGLALKEGIFVLNSPGTVDSDYR
                     GEIKVILANLSDKEFAVKKGDRVAQLILAKIESARFVMADALSGTDRGTGGFGHTGT"
     gene            5656..>6654
                     /locus_tag="PKH10_04375"
     CDS             5656..>6654
                     /locus_tag="PKH10_04375"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNT27398.1"
                     /translation="MFADLRKKSGSLVIYLMFAAIIVTFVISFGPGDQGCRAQPNWVA
                     MVNGDTITQSEFRFVFNNYYDFYQRMIPEFNAQKAKEMNLSKSALDGLVEVMLLAQKA
                     EDLGFAVTDEEIRREITETPYFLKGEAFDRDQYTRFVQFQMGMTIAAYEEKVRRDLFA
                     EKLRRFLTDSADVSESEISQEFINNNESIDAQFLVISESRMKPEAREKIAQSVEEKEI
                     AELLAKDEARAKSFLADNKERWRKDDGTERPFDEVKSDIARELIVKDRIAALVKAEAA
                     KALSLFSGGSYKPELLKKDFPDWDLAVQEQTAIKRTARYIPDIGISADLVKSLFSLP"
BASE COUNT         1663 a         1977 c         1982 g         1032 t
ORIGIN      
        1 acgggataac cgagagacag atagcgccgg agcgggatca ggccgtcagg gccggcaccc
       61 cggaaggaga cccaaccacg ccgccgcatc aggagccaga accatacgca ggcaacgaga
      121 taagaaacga tcgtcgcgta ggcggcgccg cgcgtttcaa gccgggggaa aggccccatg
      181 ccgaacatca gcacgggcga cagcaggatg ttcagcacga aggtcgcccc gtagacgcgc
      241 accggcatga cggtgtcgcc gcgcgctatc atgatggaat ggagagagaa cagcaggaag
      301 atgacaacgg tgaacatcgt catcacttcg agatactgga cacccgcggt cgttgcgacc
      361 ggcgacatgc cgacataggc gatgaggtcc gggatgatcg gatagatgag taccgtgagt
      421 atcgcgccgg caccgacggt atagaaaagc gcccggccgg cattcgccgc cgcacggtcg
      481 tcacgcccct cccccgcgtt gcgcgagacg ccggccgcga tgccggtcgt gatgagatgc
      541 gacagaccgt gtatcgccca gacgaggaat accgagcccg acaccccggc cagatagtcg
      601 gggccgagtc tgccgatcca gaacatgtcg acgatgttgt atcccgcctt gaggatgttg
      661 agtatgatga ccggcacggc gagcgccaac agcgtagcgc ggaccggtcc cgcggtgatg
      721 tcaagcggca ggtctcgggt tctcatgaac agacgctccg gagaaagggt tgcggcatcc
      781 taactaaaag ttgaacgaat tcaagaataa aaacccgttt tggtgaccgg cgtatagatt
      841 tttgtggaaa ttgcgcggca tcgcgtatat tcttccgccg ttgacccaag agaaagaaaa
      901 cactgcggag gcatctccat gaagcggatc gtttgttcct gcgcggtact tgccgcgata
      961 gtgctctgca cccccgcgct cgacgcggcg aaaaaaaagg gaaaggcaaa aacgaaaaag
     1021 cccggcaccg cactcgtgac cggccccaag aagtgcgacg aactcgaagc ggaattcaaa
     1081 cgaaaggtga acgacctcaa ggcgaaatgg aaactcagcg acgagggact cgaccggtcg
     1141 ctcgacgaga ccgagaggaa actgaccgac atggaccgga acggtgcggc cgaactccgg
     1201 agatacgcgg cggaactgga agggcagaaa aaacaggaac tcgacgcggc gaaaagggac
     1261 catgagaagg cgatggcgca gttcgagaag aaggacgacg gcgccaaacg ctccttcgac
     1321 atggaggcgg agcgtcatcg ccgtaagatc gccgacctcg aacggcggaa ggacgagagc
     1381 cagaaggaaa tgcaacggat cgaatcggtg cgcgaccggc tggaaacgga acgctcgatc
     1441 gaagaacagc gactgctcgc cgagtatgag aaggtcaaac tggcggctga ggacgagagc
     1501 cgcaaatacc gcaagcaggt cgaggatgaa taccagaaga aattcgacga actcaacgcg
     1561 aaacttggcg aatgggatgc caaggaggcc gaaaaacgga aagagaccga gggattcgat
     1621 cgccagatag aggaattcgc catgcagacg gcccagaaac tcgacgcatt ccagcaggag
     1681 atcgagaaga agcgagccga agcgcaggcg aaattcaagg acgaagaact cgagaaggcc
     1741 gaaatggaac tcgacatggc gctcgcccag gagtcacaac gcctgaacgc cgagatagag
     1801 agataccgca aggagcggct cgaaaagaag aaggagaagg agcagcaggt gaaggaattc
     1861 ctcgccacca aggatgactt ccggaaacgc aacgaccgtc ttgtccagat ggcccagcaa
     1921 taccgcgaca gccagctcga aaagaggaaa gccgaggaga tgaaggcaca ggaacggtgg
     1981 aacaacgaga agaaacggcg cgacgaacga tatgagcggc gcctgcagat ggagtaccgc
     2041 gagaagaggg acgcgctcgt caagaagcag gaccaattgt ctcttgagat agaacaggag
     2101 aaaagcggac tcgtgacact cgaaggcaag ttccgtcagc agaccgacag caaggaggcg
     2161 gccatagaga gcgagaaggg gcgcatcacg gaacgctaca agaagaccgt cgccaagata
     2221 caggacaaat atgacatgat ggaggtcgag gaaaaacata ctcaggaact ggtgcgcgac
     2281 caactcgagg agaagcggga ttcactcatc gccacccgca acaagaacaa ggccgaccgg
     2341 caggaactcc tctacaagat gaaggacgac tacgaggagc agaaaggttc ctgtgtgaaa
     2401 taaggcgcgg ctccgcgggg gttcgtcgga gaaaaaataa agttgacttt ggcgctcttc
     2461 ccactataat gccgtttgta ctttgttgta ttgattgatt tttctgtttc caaggagaga
     2521 acagatgttg gaaagagtcg ctaagcaggg cgttatcacg aagttcaaga cgcacgagca
     2581 ggacaccggt tccccggagg tccagatcgc gcttcttacc acccgcatca accagctgac
     2641 cgaacatttc aagttccata aaaaggacaa ccacggccgc ctcggcctgt tgaaaatggt
     2701 cggtaagcgg gcgaaactct tgagatatct caaggacaat catttcaccc gctacagcga
     2761 tctgataaaa gaactcggtc tcagaaagta gcgcacactt tgtgtcccgc tcactatcct
     2821 gaaccacacg caaaacaatg aggtgatcat gaagacccat cacgtagagt ccatcgtatt
     2881 cgagggcacc cctctttcac tggagaccgg caagtacgcg aaacaggccg acggcgccgt
     2941 atggtgccgc tacggcaatt cggtcctgct cgcgaccgcc gtggccgcga aaggcgatgt
     3001 ggcggcggaa caggatttct tcccgctgac cgtcgattat atcgagaaat tctacgcggc
     3061 ggggcggttc cccggcggct tcctcaaacg cgaaagccag ccgaacaccg acgagaaatt
     3121 agcggcccgc atcattgacc gaccgatacg ccccctgttc cccgactggt accggagcga
     3181 gacgcaactg ctcgtgaatc tcttctccca tgacgagaac tgcgacccgc gcgtcattgc
     3241 catcaccgcc gcgtcggcgg cgctcatgat ctccgatgtc cctttcctcg gccccatcgg
     3301 cggggtccgc gttgtactca aggacgggga gtacttcata aatccgaccc ctcaggctca
     3361 ggagggggcc cggctcaaca tcatcatggc ggccaaccgc gactcgatac tcatggtcga
     3421 gggcgaagcg cgggaagcga ccgaggacga gatcgccgag gcgctcgccc gcggtcatcg
     3481 ggcgaccctg ccgcttatcg agatgcagga ggcgctcatg gccaaggtcg cgccggtcaa
     3541 gcgacaggac ccgcagaacc ctgatgacgc ggcccttcgc aatttcatcg aactcttcgc
     3601 ggtggacaaa ctcaaggagg cactcgccat ccgcgagaaa ctggcgcgct atgaggcaat
     3661 aaaagaggtc aagacctcgg taggtgaacg ggtcgccgaa tcgctcgcgg gcggggccca
     3721 gttcctgaac gcggatgaac ctgagaaggt ccagaatcgc gcaaaagcca tattcgagga
     3781 tgtcctcaag aagaccatgc gccgcatgat caccgaagag ggtcgccgca tcgacggtcg
     3841 cggccccacg aacatacgcc cgatctggat agagaccggc gttctgccgc aggtccacgg
     3901 atcggcgata ttcacccgcg gcgagaccca gtcgctcggc accatcacgc tcggggtcgg
     3961 ttccgacgaa cagcgggtcg acacggtgct tgaggaaacg cgcaagacat tcatgctcca
     4021 ctataacttc ccgccgttct cggtcggcga ggtcgggcgc ctccgggcgc cgggccgtcg
     4081 cgagatcggt cacggcaatc ttgccgaacg ggcgctcaaa tccttcgtgc cgcccaagga
     4141 ggagttcgcc tacaccgtcc gcgtggtttc cgagatactg gaatcgaacg gctcctcatc
     4201 gatggcaagc gtctgctccg gttgcctcgc gctcatggat gccggcgtcc ccttcaagaa
     4261 acacgtcgcg ggcatcgcga tggggcttat caaggagggc gacgattacg cgatactctc
     4321 cgacatcatg ggtgacgaag atcacctcgg cgacatggat ttcaaggtcg ccggcaccaa
     4381 ggacggcatc accgcgatac agatggatat caagatacac ggcctcccgc aggaggtgat
     4441 gcgccgggcc ctcgcgcagg cgcgcgacgg acggatccac atcatcggcg agatggaaaa
     4501 ggcgctaccc gaacaccgcg cggaactttc gccgctcgcc ccgaaactgg tcaccatcca
     4561 catcaacccg gagcggatac gcgatctcat cggcaccggc ggcaagaata tcaaatcgat
     4621 cgtcgagaag accggcgcgg agatcgaggt ccagcaggac ggctcggtgc tcatcgcggc
     4681 caaggacggc gcagtactcg acgcggccat cgcgatggtc aagtccttca ccgacgaacc
     4741 ggaaaagggc aagacctacg aaggggtggt cacccgcatc gaggattacg gcgcattcgt
     4801 cgaggtcatg ccgggacaga ccggactcct ccatgtcagc cgcatagccg acaaacgcat
     4861 cgagagcgtc agcgatgtcc tcaaactcgg cgacacggtc cgcgtcatcg tcgtcgaact
     4921 cgagggggcc ggcaaattca aactcagcat gaagccctcc gacttcgagc gcgatttcac
     4981 caaggacgac ggcgaggagc cgcgccgcga acggcgcgac cgcaaaccgc acggcgaccg
     5041 cgaccgcgat cgcggtcgtg accgcgaccg tgaccgtaaa ccccacggcg accggaaaga
     5101 ccgctcataa tatgaccgac ggcgtcaccg tcaagataac ggccgctccc ggcctgcccc
     5161 tcccctccta ccagaccgcc ggctcctctg gtgccgatgt ccgcgcgtcg accgacacca
     5221 tcataccgcc gcgcggctgg gccgccgtcc cgacggggct cttctgcgag ataccgtccg
     5281 gctgggagat acaggtccgt tcgcgatcgg gactcgcgct caaggaaggc atattcgtcc
     5341 tcaactcgcc cggcaccgtc gacagtgact accgcggtga gataaaggtc atcctcgcca
     5401 atctttccga caaagagttc gccgtaaaaa agggcgaccg cgtcgcgcaa ctcatcctcg
     5461 cgaagatcga atcggcgcgc ttcgtcatgg ccgatgctct ctccggcacc gatcgcggca
     5521 caggcggttt cggccacacc ggcacatagt agaactctcc cgcgatatcc acccaccgaa
     5581 aagttgcttt ttggcataaa cccgctagaa tgccccctgc ttgattagtt gtttagaaaa
     5641 gtcaagaggt tagtcatgtt cgccgatctg aggaaaaaaa gcggttctct cgtcatctac
     5701 ctgatgttcg ccgccatcat cgtcaccttc gtcatcagtt tcgggcccgg cgatcagggc
     5761 tgtcgcgccc agccgaactg ggtcgcgatg gtcaacggcg ataccatcac gcagtcggag
     5821 ttccgcttcg tattcaacaa ctattatgat ttctatcagc gcatgatccc agaattcaac
     5881 gcgcagaagg ccaaggagat gaacctctcc aagtcggcgc tcgacggcct cgtcgaggtc
     5941 atgctactcg cccagaaggc cgaggacctc ggctttgcag tgaccgacga ggagatacgc
     6001 cgcgagataa ccgagacccc ctatttcctg aagggagagg ccttcgaccg cgaccagtat
     6061 acccggttcg tccagttcca gatggggatg acgatagcgg cgtacgagga aaaggtgcgc
     6121 cgcgacctgt tcgccgagaa actccgccgg ttcctgaccg attcggccga tgtgtcggag
     6181 agcgagatat cgcaggaatt catcaacaac aatgaatcga tcgacgccca attcctcgtc
     6241 atatccgaga gccgcatgaa acccgaagcg cgcgaaaaga tagcgcagtc ggtcgaggag
     6301 aaggagatcg ccgaactgct cgccaaggac gaggcgcgcg ccaagtcgtt cctcgccgac
     6361 aacaaggagc gctggcggaa agatgacggc acggagcgtc cgttcgacga agtgaaaagc
     6421 gatatcgccc gcgaactgat cgtcaaggac cgcatcgccg ctctggtgaa ggccgaagcg
     6481 gccaaggcgc tctcgctctt ctccggcggt tcctacaaac cggaacttct caaaaaggac
     6541 ttccccgact gggacctcgc cgtacaggaa cagaccgcca tcaagcgaac ggcccgctat
     6601 atccccgaca tcggcatatc ggccgacctc gtgaagagcc tcttctccct gccc
//
LOCUS       DAOQUE010000101         6651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12683_length_6651_cov_4.278351, whole genome shotgun sequence.
ACCESSION   DAOQUE010000101 DAOQUE010000000
VERSION     DAOQUE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12683_length_6651_cov_4.278351"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKH10_04380"
     CDS             <1..338
                     /locus_tag="PKH10_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27399.1"
                     /translation="IDYEMIDKLQFGVRFDLNYGIYSLDAASGNYLTWYTGAGTTKVN
                     YFKNDVAQMRFGIGINLFAKEFEGVHSFAGFTFMMQPQVQIVNLTGTANDQYNYGFYS
                     IGLNGGAEF"
     gene            451..1092
                     /locus_tag="PKH10_04385"
     CDS             451..1092
                     /locus_tag="PKH10_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27400.1"
                     /translation="MKRMTLGIMAVTIVTCAWLLCSCSGKQETDRREEVQKMVEQGQY
                     APAKQLIIALRSNYPNDQKLLDLEHLCDAKIAEQKYNDIWKEAESKDDYQEWIKAMIR
                     IKEVENFNRDMVKEWIQKATTKAIDAGARQLNDEELLGLLNKLYMRYQVISDKDRLMH
                     ITLFHNDGRFPIKEWQDTFVSKYPELLDEKGNFVGYPRPEKPADTKAAKTPAK"
     gene            1117..2220
                     /locus_tag="PKH10_04390"
     CDS             1117..2220
                     /locus_tag="PKH10_04390"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNT27401.1"
                     /translation="MTLVTIRKIISSGEGIGWSDGVTYFVPGVIPGETVEILADRPVR
                     RARRATDLRVVEPVPLRKEPPCPLFGRCGGCDLLHMPYELQRETKLSILKDLFHQQKV
                     QLAIEPQFVPLPEFGLRTRTKVRILDGLPTFSRRASHERIPFDRCPLIHPALNDIVRT
                     DAAARKGGEISYEYAPRSGGWSPRDPMVEKWVKNESFCVSHGSFFQASEEGASALVDL
                     LERTLDEERPATMLDLFCGVGLFSRFAARRGVTVTGVELSESAAADFTANLGNGARFV
                     RADLERDTELPDADLVVADPPRTGLPERLATAIVSSGAGTVLLVSCDAATFVRDTERL
                     RRAGRFTLRSLTIVDQFPATRHLEVAALLKRET"
     gene            2282..2641
                     /locus_tag="PKH10_04395"
     CDS             2282..2641
                     /locus_tag="PKH10_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT27402.1"
                     /translation="MKRVLIVDDEVALVKSMKNFFQFKGVDALIALTGEEALEALKKE
                     EGIALVITDVIMPGISGIELLKRIKEMDPAIHVIVMTGMLSIENTVSSLQNGASDYLL
                     KPFSSLDYVWEVVKGYL"
     gene            complement(2632..3660)
                     /gene="plsX"
                     /locus_tag="PKH10_04400"
     CDS             complement(2632..3660)
                     /gene="plsX"
                     /locus_tag="PKH10_04400"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNT27403.1"
                     /translation="MYATIVVDAMGGDQGPVVPVTASLEVCRQNRLIHIILVGREPEI
                     WQALPAGGSLPRNLEIVHAPDRITMDDSPSAIVRKKSRSSFHVGMKLVAEKKGDAFFT
                     VGNTGAAVAVAYFISGALPGISRPALAVVFPTLSQRKVVLLDLGATMEIKPDTLLHFA
                     VLGEAFSHCITGTASPRVKLLSVGEEQAKGRPVIIEAADRIAEKLNYGGFVEGNRLFK
                     SPDADVVVTDGFTGNVSLKVIEGLSETIYDLFRAKLAPHRYWTKVPGFLFKALFGSTF
                     QRFDYSRYGSAMLLGINHLVGIGHGRSNVGAFKSGILTLARYADEGIQMKLAKRVAAT
                     VARTDRHR"
     gene            complement(3670..3855)
                     /gene="rpmF"
                     /locus_tag="PKH10_04405"
     CDS             complement(3670..3855)
                     /gene="rpmF"
                     /locus_tag="PKH10_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNT27404.1"
                     /translation="MATVKRKHSRARGRTRRAGHPKLEAAATRACPKCGETMMPHRIC
                     PSCGNYRGKDFKGDDGK"
     gene            complement(3878..4312)
                     /locus_tag="PKH10_04410"
     CDS             complement(3878..4312)
                     /locus_tag="PKH10_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNT27405.1"
                     /translation="MLLYLPDITESERRLAADELFFGESFAPGVTLRGYHLAVAHYRA
                     GDAISLKIAGDMALEASCDRCGAPLEVSVELDEPFILFPASAGEADYVYGDEAIELDP
                     FIHEAIVLNIPPKILCSEDCRGLCPTCGRNLNDAACGCKKND"
     gene            complement(4382..>6651)
                     /locus_tag="PKH10_04415"
     CDS             complement(4382..>6651)
                     /locus_tag="PKH10_04415"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT27406.1"
                     /translation="FCQEKGFEAIDFGVSITKKGFNIYLAGDPGTGKTSSVKKYLEKM
                     AQKMPVPDDICYVHNFDMPDEPRLILLPAGSGEVLVKDMADLLDYLQYQIPRQLESET
                     FEIKKSEVMRWYQEESERNYRGIEAEAAKLNFVIKSTQGGFVINPVVGGEPIGKEEFD
                     KLGEKEKETVRTHEEKLQEKLIAFFHRERVLEKEYKDKMTQLRQSMVRLIVAEPLGEL
                     RKRYKKNEQLKSHIESLEKHLLENFDDFFTYRDITEGKAEAQNAEMPDFREYKVNLLV
                     NNKNLTGAPVIYETNPTFQNIMGYFEYEEVHNSIFTDFTKMKPGALHYANGGFLIVQA
                     NDILKNYYAWTALKRSMRNRLLRMDDIDVDFRYRVNIFPKPEAVPLNVKLIMIGDDYL
                     YHLLYTYDDDFIRIFRVKADFESTTDITARNTEGLLRFIARVVQEDCSLDFDVTALRR
                     ILHYSSRQAEDQKKYRVGASDLVDIILESDFWARKDKRHLVDEAAVRKAIAIKESRHA
                     SIKKHYYESIDRDIILLDVRGEKVGMINGLAVYSVGDFSFGVPSRITARVFAGRKEII
                     NIDREVRLSGRIHDKGSLILAGFIGDLFAQDKTLSISASITFEQNYGGIEGDSASSTE
                     LYALLSALSDLPIKQSIAVTGSVNQMGDIQPIGGVNEKVEGFFEICKMKGLTGKQGVI
                     IPKQNIEHLNLNDDVCAAIKARKFHLYAVANICEGIEILTGVPSGKRLKNGRFTPGSV
                     FARVDEKIRRLSRVK"
BASE COUNT         1287 a         2032 c         1942 g         1390 t
ORIGIN      
        1 cgatagacta cgagatgata gacaaactcc agttcggcgt ccggttcgac ctcaactacg
       61 gcatatacag cctcgacgcc gcgagcggca actacctgac ctggtacacc ggcgcgggca
      121 ccaccaaggt caattatttc aagaatgatg tcgcgcagat gcgtttcggc atagggatca
      181 acctgttcgc caaggagttc gaaggggtcc actcgttcgc cgggttcacc ttcatgatgc
      241 aaccgcaggt acagatcgtg aacctgaccg gcacagccaa cgaccagtac aactacggat
      301 tctacagcat cggtctcaac ggcggcgccg agttctgata gcgtcttttc gatctttttc
      361 acccggcatc aaaacttttt ttgacatcgg ctccccgctc ccctatgata cgggatgttc
      421 gcttgtggtc tttagaggag gatgaacgac atgaaacgga tgaccttggg tatcatggcg
      481 gtcaccatcg tgacctgcgc atggcttctg tgctcctgtt cgggcaagca ggagaccgac
      541 cggcgcgagg aggtgcagaa gatggtggaa cagggacagt acgctccggc caaacaactc
      601 atcatcgccc tgcggagtaa ctaccccaac gaccagaagt tgctcgacct cgagcacctc
      661 tgcgacgcca agatcgccga acagaagtac aacgacatct ggaaagaggc cgagtcgaag
      721 gacgactatc aggagtggat caaggcgatg atccgcatca aggaggtcga gaacttcaac
      781 cgcgacatgg tcaaagaatg gatacagaaa gcgacgacca aggcgataga tgccggcgcg
      841 cgtcaactca acgatgagga attactcggg cttctgaaca aactctacat gcgctatcag
      901 gtcatatcgg acaaggaccg gctcatgcac atcacgctgt tccataatga cggtcggttc
      961 cccatcaagg aatggcagga cacttttgtc tctaagtatc cggaacttct cgacgaaaaa
     1021 gggaatttcg tcgggtatcc gcgccccgaa aaaccggccg ataccaaagc ggcgaagacc
     1081 cccgccaagt aaccgctctc cttttcggta acgaccttga ccctcgtcac catccggaag
     1141 atcatctctt cgggcgaggg catcggatgg agcgatggtg tcacctattt cgttcccggc
     1201 gtcattcccg gcgaaacggt tgagatactc gctgaccgac cggtccgacg ggcccgccgc
     1261 gccaccgatc tccgcgtggt cgaaccggtt ccgctccgca aagaaccgcc ctgtccgctc
     1321 ttcggccggt gcggtggttg cgacctcctg cacatgccgt acgaactcca acgcgagacc
     1381 aaactgtcaa tactgaagga ccttttccat cagcagaagg tccagctcgc tatcgagccg
     1441 cagttcgtcc cgctccccga gttcggtctg cgcacccgga cgaaggtgcg tatactcgac
     1501 ggcctgccga ccttcagccg acgcgcctct catgaaagga tccccttcga ccggtgtccg
     1561 ctcatccacc ccgctctcaa cgacatagtc cggaccgatg cggcggcaag aaagggcggc
     1621 gaaatatcct acgaatatgc gccccggtcg ggcggctggt cgccacgcga cccgatggtc
     1681 gagaaatggg tgaagaacga gtccttctgc gtttcgcacg gcagcttctt tcaggcatcc
     1741 gaggagggtg cctccgcact cgtcgacctt ttggaacgga cgcttgatga agaaaggccc
     1801 gccacgatgc tcgacctgtt ctgcggggtg gggctcttct ctcggttcgc cgcccgccgc
     1861 ggcgtcacgg tgaccggcgt ggaactttcg gaaagcgccg cggccgattt tacggccaac
     1921 ctcggtaacg gtgctcggtt cgtccgggcc gatctcgaac gagacaccga acttccggac
     1981 gccgatctcg tggtcgccga tccgccgcgg accggcctgc cggaaagact cgcgacggcg
     2041 atagtctcat ccggcgccgg aacggtgctc ctcgtctcct gcgacgcggc gaccttcgtg
     2101 cgcgacaccg aacggctccg gcgcgccggc cgcttcaccc tgcggtcgct cacgatcgtc
     2161 gaccagttcc ccgccacacg gcatctcgag gtcgcggcgc tcctcaaacg agaaacttga
     2221 caacacccca gcccctatta tcattcctct tagtttatgg aacgccttgt gaggggatcg
     2281 catgaaacgg gtgctcatcg ttgatgatga ggtcgctctc gttaaatcga tgaagaattt
     2341 cttccagttc aaaggggtgg acgccctcat cgccctgacc ggcgaagagg cgctcgaggc
     2401 actcaagaag gaggagggga tcgcgctggt cattaccgat gtcatcatgc ccggcatcag
     2461 cggcatcgaa ctgctcaagc gcatcaagga gatggacccg gccatccatg tcatcgtgat
     2521 gaccggaatg ctctcgatcg agaataccgt ctcctcgctc cagaacggcg caagcgacta
     2581 tctgctcaag cccttttcga gcctcgacta tgtgtgggag gtcgttaagg gctacctgtg
     2641 acggtccgtg cgggcgaccg ttgccgccac ccgcttcgcc aacttcattt ggatgccttc
     2701 atcggcatag cgcgccagcg tcagtatacc gctcttgaac gcaccgacat tcgaccgccc
     2761 gtgcccgata ccgaccagat ggtttatgcc gaggagcatg gcgctgccgt agcggctgta
     2821 gtcgaatcgc tgaaaggtcg atccgaacag agccttgaac aggaaacccg gcaccttcgt
     2881 ccagtaccgg tggggcgcca gtttggcgcg gaacaggtcg tatatggtct cggaaaggcc
     2941 ttcgatgacc ttgagcgaca cgttcccggt aaagccgtcg gtgacgacga catcggcatc
     3001 gggactcttg aacaggcggt taccttcgac gaagccgccg tagttgagtt tttccgcgat
     3061 gcgatcggcc gcctcgatga tgaccggccg tcctttggcc tgctcctcgc ccacgctgag
     3121 cagtttgacc cgcggggagg cggtaccggt gatgcagtgc gagaacgcct cgccgagcac
     3181 cgcgaaatgc aggagcgtgt cgggttttat ctccatcgtc gcaccgaggt ccagcagaac
     3241 gaccttgcgc tggctcaggg tggggaacac gacggcaagc gcgggccgcg atatgccggg
     3301 aagcgctccc gagatgaaat aggcgaccgc gaccgccgcg ccggtgttgc cgaccgtgaa
     3361 gaaggcgtcg cctttcttct cggcgaccag tttcatgccg acatggaacg acgaccggct
     3421 tttcttgcgg acgatcgccg aggggctgtc atccatcgtg atgcggtcgg gagcgtggac
     3481 gatctcgagg ttgcgcggca gggaaccgcc cgccggcaac gcctgccata tctccggttc
     3541 ccgcccgaca aggataatgt gtatcaggcg gttctggcgg cagacctcaa gcgacgcggt
     3601 caccgggacc accggtccct gatccccccc catcgcgtcg acgacaatgg tggcgtacat
     3661 gccgggcggt tacttcccgt cgtcgccttt gaaatccttg ccgcggtagt tgccgcagga
     3721 gggacagatg cggtgcggca tcatcgtctc accgcacttg gggcaggctc gcgtcgcggc
     3781 cgcttccagt ttcgggtgcc ccgcgcggcg ggtacgaccc cgggcccgcg aatgtttgcg
     3841 ttttaccgtc gccatgacag acctccgttg ctatcggtca atcgttcttt ttacagccgc
     3901 acgcggcatc gttgagattc ctgccgcatg tcgggcacag cccccgacaa tcctcggaac
     3961 agagtatctt cgggggtatg ttcaacacta tcgcctcgtg aatgaaagga tccaactcta
     4021 tcgcctcgtc gccatacacg taatcggcct ctcccgccga cgccgggaac aggatgaacg
     4081 gctcatccag ttcgaccgac acctcgagcg gcgcgccgca gcggtcgcac gatgcctcca
     4141 gcgccatgtc gcccgctatc ttgagcgata tcgcgtcgcc ggcgcggtag tgcgccaccg
     4201 ccaaatggta cccgcgcagg gtcacccccg gcgcgaacga ctcgccgaag aacagctcat
     4261 cggccgcgag tcgccgttcg ctctccgtga tatcgggcag atacagtaac acgcgaccct
     4321 cgcggacacc ggcaaaaaac gaagagggac tatattcaca aacgaggggg tgtcaagata
     4381 tctacttcac ccggctgagg cggcggatct tttcatcgac ccgtgcgaag acgctgccgg
     4441 gggtgaaccg gccgttcttg agccgtttgc cgctcgggac gccggtgagt atctctatcc
     4501 cttcgcagat gttggccacc gcgtagagat ggaacttccg cgcctttatc gccgcgcaga
     4561 catcgtcgtt gaggttgaga tgctcgatat tctgtttggg gatgatgacc ccctgtttgc
     4621 cggtgagccc cttcatcttg cagatctcga agaagccctc gaccttttca ttgacaccgc
     4681 cgatcggctg tatgtccccc atctgattga ccgatccggt cacggcgatc gactgtttga
     4741 tggggaggtc ggagagcgcc gacagaagcg cgtagagttc ggttgaactc gcgctgtcgc
     4801 cctcgatgcc gccatagttc tgttcaaagg tgatggaggc cgatatcgag agcgtcttat
     4861 cctgcgcgaa caggtcgccg atgaacccgg cgaggatgag cgagcccttg tcgtggatgc
     4921 ggcccgacag gcgcacctcg cggtcgatgt tgatgatctc cttgcgcccc gcgaaaacgc
     4981 gcgccgtgat gcgcgacggc acgccgaaac tgaaatcgcc gacactgtag acggcgagcc
     5041 cgtttatcat gccgaccttt tcgccgcgca cgtcgagcag gatgatgtcg cggtctatcg
     5101 attcgtagta gtgtttcttg atgctcgcat ggcggctctc ctttatcgct atcgccttgc
     5161 ggaccgccgc ctcgtcaacg aggtgccgtt tatccttgcg cgcccagaaa tcgctttcaa
     5221 ggatgatgtc caccagatcg gaggcgccga cgcgatattt cttctggtcc tccgcctgcc
     5281 gcgaggaata gtgcaggatg cgccgcagcg cggtcacatc gaagtcgagc gaacagtcct
     5341 cctgcacgac ccgcgcgatg aagcgcaaca gcccctcggt gtttcgcgcg gtgatgtcgg
     5401 tggtcgactc gaaatcggcc ttgacgcgga atatgcggat gaaatcatcg tcgtaggtgt
     5461 agagcagatg atatagatag tcgtcgccga tcatgatgag cttcacattg agcggcaccg
     5521 cctccggctt ggggaatatg ttgacgcggt aacggaaatc gacatcgatg tcgtccatcc
     5581 ggagcaggcg gttgcgcata gagcgtttga gcgccgtcca cgcgtagtag ttcttgagta
     5641 tgtcattggc ctgtacgatg aggaagccgc cgttcgcgta gtggagcgcg cccggcttca
     5701 tcttggtgaa gtcggtgaag atcgagttgt gcacctcctc atactcgaag tagcccatga
     5761 tgttctggaa ggtcgggttg gtctcgtata tcaccggcgc gccggtcagg ttcttgttgt
     5821 tgacgagcag gttgaccttg tactcgcgga aatcgggcat ctcggcgttc tgcgcctcgg
     5881 ccttaccctc ggtgatgtcg cggtaggtga agaagtcgtc gaaattctcg agcaggtgct
     5941 tctccagcga ctcgatgtgg ctcttgagtt gttcgttctt cttgtatctc ttgcgcagtt
     6001 cgccgagcgg ctcggcgacg atgagccgca ccatgctctg gcgcagttgg gtcatcttgt
     6061 ccttatattc cttctccagc acccgttcgc ggtggaagaa cgctatgagt ttctcctgca
     6121 gtttctcctc gtgggtccgg acggtctcct tttccttttc gcccagtttg tcgaattcct
     6181 ccttgccgat cggctcgccg cccacgaccg gattgatgac gaacccgccc tgcgtcgatt
     6241 tgatgacgaa attcagtttc gccgcctccg cctcgatgcc ccggtagttg cgctcgctct
     6301 cctcctgata ccagcgcatc acctcgctct tttttatctc gaaggtctcg ctctcgagtt
     6361 ggcgcggtat ctggtactgg agatagtcga gcaggtcggc catatccttg acgagcacct
     6421 cgcccgaccc ggcgggcaga agtatgagcc gcggttcgtc gggcatgtcg aagttgtgga
     6481 catagcagat gtcgtcgggt accggcatct tctgcgccat cttctcgaga tacttcttga
     6541 cgctcgaggt cttgccggtg ccggggtcgc cggcgaggta gatgttgaat cccttcttcg
     6601 ttatcgagac gccgaaatct atggcctcga agcccttttc ctgacagaat g
//
LOCUS       DAOQUE010000102         6622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12752_length_6622_cov_4.425765, whole genome shotgun sequence.
ACCESSION   DAOQUE010000102 DAOQUE010000000
VERSION     DAOQUE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12752_length_6622_cov_4.425765"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PKH10_04420"
     CDS             <1..191
                     /locus_tag="PKH10_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27407.1"
                     /translation="KVSTNNEVPSYCGNGIVEPGEVCDGNSVYCEDLDPNEWNSGTAT
                     CNSTCSGWNTGNCYWSGC"
     gene            376..1956
                     /locus_tag="PKH10_04425"
     CDS             376..1956
                     /locus_tag="PKH10_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27408.1"
                     /translation="MHYCIEIAGNDIDLVNEGDDTVLSEENGLAQDDAESISDYEGVD
                     CDPWVPDGDPYADIESAAMKWHGDFDVVATDPDNIRELWKGIYRYSTDSPDPRLVKPY
                     DRCAKDYPFEPAMVEDENNFPDTYCNTNDPKADIREFPCDTLIGPGRCWAMNWTMGSD
                     PQWYENNGKVIVHLFNPNNTFFVQGEGTYLFDIATRRMTRLAGQSHFINATNGRYAFF
                     STYDNAKQYNWPEKPEYYAGDKPYSLLTYYDTEENEYGIAYRKGWRGYFSDLRASERY
                     LIATIQDDLTVSGIHVEYTKIGEWDNWKPFTAHSDPSLNRIGPATFHDHYIAFYNWDF
                     VIQYCDLDIGSDSCKVISRYGEQEGKYNRNATLSRDGKRIYYDEGGDGYQNRAGTIIE
                     ADITDLNNITYREIAQHYNAIGVHDIDDRFLLFGKQTGLEEGKLSENPHYVACFHRFS
                     DGRNFCLENDHDSDLSGDVSKAVAQLWDKYLVYGNNSGALVLRDMKCYCNAYPDTCPY
                     EEYAPPRKGWKNLFGLIR"
     gene            2096..3685
                     /locus_tag="PKH10_04430"
     CDS             2096..3685
                     /locus_tag="PKH10_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT27409.1"
                     /translation="MMLMGDEAIAQGAIDAGVSGFYAYPGTPSTEIMEYAQHSKDAAA
                     LNIHRQWSANEKTAMESALGMSYCGKRAMVSMKHVGLNVAADPFVNSGVTGANGGIIV
                     VAADDPSMHSSQDEQDSRILAKFALLPIFEPANQQEAYDMAFAGFELSEKYKTPVMLR
                     LTTRLAHSRAGVAQKSRLSQKELKLPENKRQFVLLPGIARKQYKTLVANQAKFMEEAE
                     KSPFNRYIDGKDKSRGIIACGLAYNYLMENYPDGCPHPVIKLCQYPVPKKMVEKLVNE
                     TKEILVLEDGQPMVEEALRGLLDRDVKVKGRITGELPRDGELNPLFVRRALGMADICG
                     QPAPAIVAGRPPAFCVGCGHIDAYNAIKQVVTEGYGDGHVFADIGCYTLAALPPFEVV
                     NSCVDMGASITMAKGAADAGLFPACAVIGDSTFCHSGMTGLLDCVIENANVVIFILDN
                     SATAMTGGQDSHATGRLSEICKGLGVDEKHIRIVTPLKKNHDEMVQVIKEEIEHKGVS
                     VIIPTRECIQTLGKKNKKGGE"
     gene            3685..4269
                     /locus_tag="PKH10_04435"
     CDS             3685..4269
                     /locus_tag="PKH10_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNT27410.1"
                     /translation="MRSDIILSGVGGQGILSIAAVIGQAAISAGLHMKQSEVHGMSQR
                     GGDVQSHLRISDKEIFSDLVPHGGADMILSVEPMESLRYLPWLSKQGWIITNSQPFVN
                     IPNYPDAKAVIAEIEKQPKHIIVNADELAKAAGNSRGMNMVMAGAASLFIDIPQEKME
                     GAIEFLFGRKGPEIVKTNIAAFRAGREFAEKKRG"
     gene            complement(4274..5947)
                     /locus_tag="PKH10_04440"
     CDS             complement(4274..5947)
                     /locus_tag="PKH10_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNT27411.1"
                     /translation="MAEEHSVQMIFPDGKTEEIQLSPGAHLKTLADRFADRFDLPILG
                     AICDNRVVSLEGKVNTFKSIEFITMRDKNGRLIFLRSLLFVFIKAAKEMFPHAQLHIE
                     YGIGNGLYCYFEGLEWIRLEEVEDLEKKMREIVAQDLRFIRKEMPTPEAVRLFKEAGL
                     QDKVRLFSTRTDETSSVYYLGGLIDYFYGHLAYSTAAITDFRLQYYNRGIVLLIPDQN
                     DPTKVEAFKDQPKFFNIITENKKWLDILDLENCGQLNEAVRRGRAKEIVLVQESLHEK
                     KLAFIADEIHRRREVVRVVSIAGPSSSGKTTTAKRLAIQLRVNGFRPLLISLDNYFVD
                     RDKTPKDASGKHDFEGIDAIDVALFNEQVLRLLQGKGTKLQRYDFLTGKSARGETKYR
                     LPRNGVIIIEGIHGLNPQLFTDLNYDRTYKIYVSALANLNIDHHNRIPTTDCRLIRRM
                     VRDAQFRGHDAIETIDRWPSVRRGEERNIFPYQERADIMFNSSLVYELSVLRRYAEPL
                     LQKITPDRREHLEARRLLKFLSFFEPIEEGFIPFNSIIREFIGGSAFDY"
     gene            complement(6022..>6622)
                     /gene="bioB"
                     /locus_tag="PKH10_04445"
     CDS             complement(6022..>6622)
                     /gene="bioB"
                     /locus_tag="PKH10_04445"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNT27412.1"
                     /translation="GSTLRHFAAEKIPQRPCASLGILSADELRSLKAAGLKRYHHNLE
                     VSRNFFPQLCTTHTYDERVATVRAAQEAGLETCVGGIFGVGESVEDRIDLLFEIAELA
                     PDSVPLNFLVPIAGTPVGHLPPFDMFEAVKLIALARFILPDKDIKVGGGRIEVFGDSQ
                     VLVFLAGANSIITGDLLTVKGRRSADDIKLLADLGLTPT"
BASE COUNT         1428 a         1883 c         2005 g         1306 t
ORIGIN      
        1 tgaaggtctc gacgaacaac gaggtgccga gttactgcgg gaacgggatc gtggagccgg
       61 gcgaggtatg cgacgggaac agcgtctact gcgaggatct cgatccgaac gagtggaaca
      121 gcggtacggc gacttgcaac tcgacctgta gcggatggaa tacgggaaat tgctactggt
      181 cggggtgcta agatgagaaa gatatttact actaggggcg gcgtggccgc ccctttggtt
      241 ttttttctga tggttgcttg tgccccctcg aatccggcac cgacagacga cagtgcatct
      301 tcatgtgccg gtcggtgcgg aatggtgaac ggaattgcat gcggcccctg tggttacgga
      361 tatatttgca atgaaatgca ctactgcatc gagatagcgg ggaacgacat cgacctcgta
      421 aatgaaggcg acgataccgt gttgtcggaa gaaaatggtt tggcacaaga tgatgcggag
      481 tcgatttccg attacgaggg cgtcgattgc gatccgtggg tgcccgacgg cgatccctat
      541 gccgatatcg agtcggccgc aatgaaatgg catggcgatt tcgatgtggt ggcgaccgac
      601 cccgacaaca tccgggaatt atggaaaggg atttatcgtt atagcaccga ctctcccgat
      661 ccccgtctgg ttaaacccta cgaccgatgc gcgaaggatt acccctttga accggcgatg
      721 gtggaggatg agaataactt tcccgacacc tattgcaata ccaacgaccc caaggccgac
      781 atccgggaat tcccctgcga taccctgatc ggccccggtc gttgctgggc gatgaactgg
      841 accatgggga gcgatccaca gtggtacgaa aacaacggta aggtgatcgt gcatcttttc
      901 aatcctaaca acaccttttt cgtgcaaggt gaggggacct atctgttcga catcgcgaca
      961 cgacggatga cccgccttgc cggccagtca cactttatca acgccacgaa cggtcgctac
     1021 gcctttttca gcacctacga caacgccaag cagtacaatt ggcccgagaa accggaatat
     1081 tacgcgggcg acaaaccgta tagtttgctc acctactacg acaccgaaga gaacgagtac
     1141 gggatcgcct atcgcaaggg ctggcgggga tatttttccg atctgcgcgc cagcgagcgg
     1201 tatctcatcg ccacgataca ggacgatctg accgtaagcg gcatccatgt cgagtacact
     1261 aagatcggtg agtgggacaa ctggaaaccg ttcacggccc acagcgaccc atcgcttaac
     1321 cggatcggtc cggcaacctt tcatgaccat tatatcgcct tttacaactg ggatttcgtc
     1381 atacagtact gcgatctgga tatcgggagc gattcgtgca aagtgatatc ccgttatggt
     1441 gaacaggaag gaaaatataa ccgcaatgct acccttagcc gtgacggaaa acggatatat
     1501 tacgacgaag ggggggacgg ttatcaaaat cgtgcaggca ccatcataga ggccgatatc
     1561 accgatttga acaacattac ctatcgggag atcgcgcaac actacaatgc gataggagtt
     1621 cacgatatcg acgaccggtt cctgctgttt ggtaagcaga caggcctcga ggaggggaaa
     1681 ctcagcgaaa acccccatta tgtggcctgt tttcatcgat tttccgacgg caggaatttc
     1741 tgtctggaga acgatcacga cagcgatctt tcgggagatg tcagtaaggc ggtagcgcag
     1801 ttgtgggaca agtatctggt ctatggcaac aacagcggtg cactcgttct ccgcgacatg
     1861 aaatgttact gcaacgccta tcccgacacc tgtccgtatg aggaatatgc gccgccgcga
     1921 aagggctgga agaacttgtt cgggttgatc aggtagtttt tcttttcccc cttgtttctt
     1981 ctgtttttcc caaaaggtat aaaattttct tgaaaaacgg cgcctgccgg aagtagagtg
     2041 aaaggcaaga acgaacaaca acccggcatg gaggtgtgtc ttggccaaag agaagatgat
     2101 gctgatggga gacgaggcga tagcgcaggg cgccatcgac gccggtgttt ccgggttcta
     2161 tgcctacccc ggcaccccct cgaccgagat catggaatac gcccagcact cgaaggatgc
     2221 cgccgcgctg aacatccacc ggcagtggtc ggccaacgag aagacggcga tggagtcggc
     2281 gctcgggatg tcgtactgcg gcaaacgggc gatggtgtcg atgaagcatg tcgggctcaa
     2341 tgtcgcggcc gatcccttcg tcaactcggg cgtcaccggc gcgaacggcg gcatcatcgt
     2401 cgtcgcggcc gacgacccct cgatgcactc ctcgcaggac gagcaggaca gccggatact
     2461 cgcgaagttc gcgctcctgc ctatcttcga gccggccaac cagcaggagg cctacgacat
     2521 ggccttcgcc gggttcgaac tctcggaaaa gtacaagacg ccggtcatgc tccggctcac
     2581 cacgcggctc gcccacagcc gcgccggggt ggcgcagaaa agccgcctgt cccagaagga
     2641 actgaaactg cccgagaaca agcgacagtt cgtcctcctg ccggggatcg cgcgcaaaca
     2701 atacaaaaca ctcgtcgcga atcaggcgaa attcatggag gaggccgaga agagcccctt
     2761 caaccgctat atcgacggga aagacaaaag ccgcggcatc atcgcctgcg gactcgctta
     2821 caactacctg atggagaact accccgacgg ctgtccccat ccggtcatca aactctgcca
     2881 gtacccggtc cccaagaaga tggtggaaaa actggtgaac gagaccaaag agatactcgt
     2941 cctcgaggat ggtcagccga tggtcgagga ggcgttgcgc ggcctgctgg accgcgatgt
     3001 caaggtgaag ggccgtatca ccggcgaact gccgcgtgac ggcgaactca acccgctttt
     3061 cgtccgccgc gcgctcggca tggccgacat ctgcggccag ccggctcccg ccatcgtcgc
     3121 cggtcgtccc cccgctttct gtgtcgggtg cggtcacatc gacgcctaca acgccatcaa
     3181 gcaggtggtc accgagggtt acggcgacgg ccatgtcttc gccgacatcg ggtgctacac
     3241 cctcgcggcg ctcccgccgt tcgaggtggt caactcctgc gtcgacatgg gcgcatcgat
     3301 cacgatggcc aaaggggccg ccgacgccgg gctcttcccc gcctgcgccg tcatcggtga
     3361 ctcaactttc tgccacagcg gcatgacggg cctgctcgac tgcgtcatcg agaacgcgaa
     3421 tgtcgtcatc ttcatactcg acaactcggc taccgccatg accggcggtc aggactcgca
     3481 cgcgaccggc cggctcagtg agatctgtaa agggctcggc gttgacgaaa agcacatccg
     3541 catcgtgacg ccgctcaaga agaaccacga cgagatggta caggtcatca aggaggagat
     3601 cgagcacaag ggtgtctcgg tcatcatccc gacgcgcgaa tgtatccaga cgctcggtaa
     3661 gaagaacaag aagggaggcg aatgatgaga agcgatatca tactttcggg cgtcggcggt
     3721 cagggcatcc tctccatcgc ggcggtcatc gggcaggcgg caatatcggc gggcctgcat
     3781 atgaagcagt cggaggtcca tgggatgtcc cagcgcggcg gcgatgtcca gtcgcacctg
     3841 cgcatcagcg acaaagagat attctccgac ctcgtgccgc acggcggcgc cgacatgata
     3901 ctctcggtcg aaccgatgga atcgctccgc tacctgccgt ggctctcgaa gcaggggtgg
     3961 atcatcacca actcccagcc cttcgtgaac atcccgaact atcccgacgc aaaggcggtc
     4021 atcgccgaga tagagaaaca gcccaaacac atcatcgtca acgccgacga actcgccaaa
     4081 gcggcgggca actcgcgtgg catgaacatg gtgatggcgg gcgccgcgtc gctcttcatc
     4141 gacatcccgc aggagaagat ggaaggggcg atcgagttcc tcttcggtcg caagggcccc
     4201 gagatcgtca agaccaacat cgccgcgttc cgcgccggtc gcgaattcgc ggagaagaaa
     4261 cgcggctgat ccctcaatag tcgaacgccg aaccgccgat gaattcccgt atgatcgaat
     4321 tgaacgggat gaaaccctcc tctatcggtt cgaagaacga aaggaacttg aggagccgcc
     4381 gcgcctcgag atgttcgcgc cgatcgggcg ttatcttctg aaggagcggc tcggcgtacc
     4441 gacggagtac cgacaattcg tagacgagcg acgagttgaa catgatgtcg gcccgttcct
     4501 gatacgggaa gatgttgcgc tcctcaccac gccgcaccga cggccagcgg tctatcgtct
     4561 cgatggcgtc gtgaccgcgg aactgcgcgt cgcggaccat gcggcggatg agccgacagt
     4621 cggtcgtcgg gatgcggttg tgatggtcga tgttcaggtt ggcgagcgcc gagacataga
     4681 tcttgtaggt gcggtcgtag ttgagatcgg tgaagagttg cgggttgagc ccgtggatgc
     4741 cctcgatgat gatgacgccg ttcctcggca ggcgatattt cgtttctccg cgcgcgctct
     4801 tgccggtgag aaaatcgtac cgctggagtt tggtcccctt cccctgcagg agccggagca
     4861 cctgttcgtt gaagagagcc acatctattg cgtcgatacc ttcaaaatcg tgtttgccgc
     4921 tcgcatcctt cggggtcttg tcgcggtcca cgaaatagtt gtcgagactg atgaggagtg
     4981 gtcggaaccc gttgacgcgc agttgtatcg cgagccgttt ggcggtcgtg gtcttccccg
     5041 acgacgacgg cccggcgatg gaaacgaccc gcacgacctc gcggcggcgg tgtatctcgt
     5101 cggcgatgaa ggcgagtttc ttctcgtgga gcgactcctg caccagcacg atctccttgg
     5161 cgcggccgcg gcgcaccgcc tcgttgagtt gcccgcagtt ctcgaggtcg aggatgtcga
     5221 gccacttctt gttctcggtg atgatgttga agaacttcgg ctgatccttg aacgcctcga
     5281 ccttggtcgg gtcgttctgg tcggggatga gcagcacgat gccgcggttg taatactgca
     5341 ggcggaaatc ggtgatggcg gcggtcgagt aggcgaggtg accgtaaaaa taatcgatga
     5401 gcccgccgag atagtagacg ctcgatgtct cgtcggtccg cgtcgagaag agccgcacct
     5461 tgtcctgcag gcccgcctcc ttgaagagcc gcaccgcctc cggcgtcggc atctccttgc
     5521 ggatgaagcg gaggtcctgt gcgacgatct cgcgcatctt cttctcgagg tcctcgacct
     5581 cttcgagccg tatccactca agaccctcga agtagcagta gagcccgttg ccgatgccgt
     5641 attcgatgtg cagttgggcg tgcgggaaca tctccttggc cgctttgatg aagacgaaca
     5701 ggagcgaccg caaaaagatg agacgcccgt tcttgtcacg catcgtgatg aactcgatgc
     5761 ttttgaaggt gttgaccttc ccttcgagcg acacgacgcg gttgtcgcag atcgcgccga
     5821 gtatcgggag gtcgaagcgg tcggcgaacc ggtcggcgag cgtcttcaga tgggcgccgg
     5881 gcgaaagttg gatctcttcg gtcttcccgt cgggaaatat catctgaacg ctgtgttctt
     5941 cggccatcgg gcgctccttc agcaatggaa agtcagtata gcacaccgtc cggtcccttg
     6001 tcaaagagac cttgacggga tttatgtcgg tgtgagtccg aggtcggcga gaagtttgat
     6061 atcgtcggcc gatctgcgcc ctttgacggt gaggaggtcg ccggtgatga tcgagttggc
     6121 gccggcgagg aagacgagca cctgcgagtc gccgaacacc tcgatgcggc cgccgccgac
     6181 cttgatgtcc ttgtcgggca ggatgaagcg ggcgagggcg atcagcttca ccgcctcgaa
     6241 catatcgaag ggcggcagat ggccgaccgg cgtccccgct atcgggacga ggaagttgag
     6301 cggtaccgaa tcgggagcga gttccgctat ctcaaagagg aggtctattc tatcttcgac
     6361 cgattcgccg acgccgaaga tgccgccgac gcaggtctcg agtcccgcct cctgcgccgc
     6421 gcggaccgtc gcgacccgct cgtcgtaggt gtgggtggtg cagagctgcg ggaagaagtt
     6481 gcgcgacacc tcgaggttat ggtggtagcg cttcagtccc gccgctttga gcgatctcag
     6541 ttcatccgcc gacaagatgc cgagcgacgc acacggcctc tgcggtatct tttccgcggc
     6601 gaaatggcgc agggtacttc cg
//
LOCUS       DAOQUE010000103         6610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12789_length_6610_cov_4.264989, whole genome shotgun sequence.
ACCESSION   DAOQUE010000103 DAOQUE010000000
VERSION     DAOQUE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12789_length_6610_cov_4.264989"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PKH10_04450"
     CDS             <1..993
                     /locus_tag="PKH10_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27413.1"
                     /translation="PGLVAGLLGPVLSGTETMGGGLSPVAGIPSLGILWNAMKNDANV
                     NVMSTPHILTTDNEEAEIVVGDTIPFPSGNIISGTAGSQITYKREDVALKLKIKPQIN
                     ESGYMTLQVEQEVTELGPMTDYGFTTSKRAAKTIINAEDQQTVVIGGLMKDIVTEGES
                     KVPILGDIPVLGALFKYTKNKKEKVNLLIILTPHIVEGKEDFERIYKRKMREREEFAK
                     KFYGSIKDYERTLTLEKKRGALLAMARLLDKDKLREEEEKERIDRMNRRRGLMITPDG
                     AQKEIDLGRKPNGVVDDDPAMMPALPPEMPSAPEDALVPEPSPVEEVPSIEESR"
     gene            995..2686
                     /gene="gspE"
                     /locus_tag="PKH10_04455"
     CDS             995..2686
                     /gene="gspE"
                     /locus_tag="PKH10_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNT27414.1"
                     /translation="MRKQIGAIVVERGYATVAFVEEALRLQQGGDARKVGEILIDMGK
                     ASVEQIHLALAEQVGLTWGEQDLLSRQDPVLLQAFPIYLARRYRAVPLFAEEDRLLAV
                     AVDPEDLVLYNQFYLIYGKNVILLPVEEKKVTELINSAYSRLDTTEGSLNAEEFRDMG
                     TDEDAALPDLIDSNDEAPIIRFVNSMVAKAVKERSSDIHFESFEDEMLVRFRRDGKLY
                     VIQKVPKAAQAGLITRVKIMARLNIAEKRLPQDGNIKIRVAGNDIDFRVSTLPSVHGE
                     SVVLRILDKTTLQLSLDNCGFNQRDLKTMRQMIQKKHGIFLVTGPTGSGKTTTLYSAL
                     TEINSPDVKIITEEDPVEYQLKGINQIHINPKIGLTFAAGLRHILRQDPDIIMVGEIR
                     DKETADIAIQASLTGHLVFSTLHTNDAPSAFTRLLDMEVEPFLISSTVIGVLAQRLIR
                     KLCLHCREAYQPEAAILRELGLDPEEYRHHTFYRAVGCEKCSHTGYQGRMGIFELLVI
                     DDDIRRAVVNRQDASVIKKTAAAAGMLTLREDGAMKAVKGITSPEEILLRTQEDT"
     gene            2687..3910
                     /gene="gspF"
                     /locus_tag="PKH10_04460"
     CDS             2687..3910
                     /gene="gspF"
                     /locus_tag="PKH10_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HNT27415.1"
                     /translation="MALYAYRIMDRDGHERKGTIEGSSRNAVKAKFQADGWFVVDIRE
                     KASREGLSLFRTVFSMRRKKVAIEEVASMTRLLATLQKARIPLVESIAAVAQQQDNPR
                     LGEVLMLVKTKMQEGYSFSRAARDFPEVFSPLYCNMIAAGEESGATEAVLLRLADFLE
                     SQMDLRNKVRSAMTYPIILFVVAIMVVALLFTVVIPKISKMFEDVNMILPLQTRILIW
                     ISGTVGSYWWLILLVAVAGYAGFHRWKKTPAGEEKWNRLLITAPIFGRINRMVAISRF
                     AKTLSTLLTSGVPILAALDIVRKVIENRVIENAVAAARENIKEGESIAVPLKRSGEFP
                     PIVIQMIAIGEKSGELEEMLETLAGSYEKEVRYSLERLTATINPIMIVFLALVIGFII
                     FAVVMPILKINEAVG"
     gene            3939..4355
                     /gene="gspG"
                     /locus_tag="PKH10_04465"
     CDS             3939..4355
                     /gene="gspG"
                     /locus_tag="PKH10_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNT27416.1"
                     /translation="MLRNIMKALFRSRAASAGFSLLEIMVVMAIMAMIMGAVGVGFMN
                     YLDKAKVKQAAMDIKTISNALDLYKSEFGKYPDGLDALVQEKILKEKKVPKDPWTNEY
                     VYINPGSNNSEGFDLYSFGKDGREGGGDDITNWEEE"
     gene            4400..5209
                     /locus_tag="PKH10_04470"
     CDS             4400..5209
                     /locus_tag="PKH10_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNT27417.1"
                     /translation="MMRYGFTMIEMLAVLAFMVIIMGVSIPAVTNISRSEAASELGIF
                     QAFVKRTFQQAVRRNEYLRLVVDMQSGDYWVEKTETPFFLMTGQEQESYDKQNQRLLE
                     EYDESERSFGSLKAGDAATSRSLFDLLGAQGDDTTYDTEDLYHWENFVPERRNIRELL
                     KPEYQPVSDRRKVPSGLQWRQFFSYHTPEVLTPEEAEEKERKAMAYVYFFPQGRISPF
                     FLSIGEKEGEPLLHIAVDMYLGIRIGKGELDEEVKGLVQKSIEEDMDEEGK"
     gene            5206..5901
                     /locus_tag="PKH10_04475"
     CDS             5206..5901
                     /locus_tag="PKH10_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNT27418.1"
                     /translation="MTVALSMIPVRGESGFSLLEVTVAIAILAGLLISMARIVSHSYY
                     YTERVADIYLGTELARLKMHEIEEEIKKDGIPTADKEDEGDFDEQAYEGFKWHYSIKR
                     VHIPLPDVSGSDLSGVESGQAQAADLVGGAKSMIEDFIKEKIRKLTLEVFWDEGKKES
                     EKVTFVMFIPADTSAKEFQQGGAAVPGAQQRPGGGQQKWFNSPTGGGGLNPATRGPAF
                     RPGTTPLPAGGPR"
     gene            5898..6551
                     /locus_tag="PKH10_04480"
     CDS             5898..6551
                     /locus_tag="PKH10_04480"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HNT27419.1"
                     /translation="MRRRGFTLIEVLVATAILALIVSSVYFSFANLIEGRTRIRERAE
                     SLRRVYFAIDLMRRDIQNAYLTRNRGTPEETHITIFEGEEDSPVTHLTFASLNHVKMR
                     AGGKESDQTEIEYYGETVDGVPTLMRRESAWVDGFPEKGGNAFPILSDFRSIAIEFWD
                     AEDRTWLSSWNSEGTDRADTLPPKVKITAQVRDADGDRPEFLVESVVSIKMQKPFTF"
BASE COUNT         1543 a         1797 c         2016 g         1254 t
ORIGIN      
        1 cccgggctcg tcgcagggtt gctcggcccg gtcctgtcgg ggaccgaaac gatgggcggc
       61 gggctcagtc cggtggcggg tatcccgtcg ctggggatcc tgtggaatgc tatgaagaac
      121 gacgcgaatg tcaatgtcat gtcgacgccg catatcctta cgaccgacaa cgaagaggcg
      181 gagatcgtgg tgggcgacac tatcccgttc ccctcgggca acatcatctc gggcacggcc
      241 ggcagtcaga tcacctacaa gcgggaagat gtcgcgctta aactcaagat aaaaccgcag
      301 atcaacgaga gcgggtacat gaccctgcag gtggagcagg aggttaccga gcttggcccg
      361 atgaccgact acgggttcac cacatcgaaa cgggccgcca aaacgatcat caacgccgag
      421 gaccagcaga cggtggtcat cggtggactc atgaaggaca tagtgaccga aggggagtcg
      481 aaggtgccga tacttggcga tattcccgtt ctcggcgctc ttttcaagta caccaagaac
      541 aagaaagaga aggtcaatct gctcatcatc cttaccccgc acatcgttga gggcaaggaa
      601 gatttcgaac ggatatataa gcgaaagatg cgcgaaagag aggagttcgc caagaaattc
      661 tacgggtcca taaaggatta cgagcgcacg ttgaccctcg aaaagaaacg aggagcgctt
      721 cttgccatgg cgcgcctgct ggacaaggac aaactgcggg aagaggagga aaaggaacgg
      781 atagatcgca tgaatcgtcg gcgcgggctt atgatcactc ccgacggagc acagaaggag
      841 atagatcttg gtcgcaagcc gaacggcgtt gtggacgatg atccggcgat gatgcccgcg
      901 cttccccccg aaatgccgag tgcgcctgag gatgccctcg tgccggagcc ctcacccgtc
      961 gaagaggtcc cgtccatcga ggagagcaga tgagatgaga aagcagatcg gcgcgatagt
     1021 tgtcgagcgg ggatacgcga cagttgcttt tgtcgaagaa gcgctccgac tccagcaggg
     1081 gggtgacgcc cgcaaggtgg gggagatact gatcgacatg ggcaaggcat ccgtggagca
     1141 gatacatctc gccctcgcgg agcaggtggg gcttacctgg ggggaacagg atcttttgtc
     1201 ccgacaggat cccgtgcttt tacaggcatt cccgatctat cttgcccgtc ggtatcgagc
     1261 ggtgccgctc tttgcggaag aggatcgtct tctagccgta gcggtcgacc ccgaggacct
     1321 agtgctttat aaccagtttt atcttatcta tggaaaaaat gtgatcctgt tgccggtcga
     1381 agaaaagaag gtgaccgaac tcatcaatag tgcctacagc cgccttgata cgacggaagg
     1441 atcgttgaat gccgaggagt tccgggatat gggcaccgat gaggatgcgg cgctgcccga
     1501 cctcatcgat tcgaatgacg aggcgccgat catccggttc gtcaacagca tggtcgcgaa
     1561 ggcggtaaag gagcggtcga gcgatatcca tttcgagtcg ttcgaggacg agatgctcgt
     1621 ccggttccgg cgcgacggca aactgtatgt catccagaag gttcccaagg cggcgcaggc
     1681 gggactcatt acccgtgtca agatcatggc gcgcctcaac atcgccgaga aacggctgcc
     1741 gcaggacggc aatatcaaga tacgggtcgc cggtaacgac atcgattttc gtgtttcgac
     1801 gcttccctcg gtccacggcg aatcggtggt cctgcgtata ctcgacaaga cgacgctcca
     1861 gttgtcgctc gacaactgcg ggttcaacca gcgtgatctc aaaacgatgc gccagatgat
     1921 acagaaaaag catggcatct tcctcgtcac cggcccgacc ggttcgggaa agacgaccac
     1981 actttactcg gcgcttacag agataaactc acccgatgtg aagatcatca ccgaggagga
     2041 tccggtcgag tatcaactta aagggatcaa ccagatacat ataaacccca agatcgggct
     2101 caccttcgcg gcgggcttgc gtcacatcct gcggcaggat cccgatatca tcatggtcgg
     2161 ggagataaga gacaaggaga cggccgacat cgcgatacag gcgtccctca caggccatct
     2221 cgtcttttcg acgctccata ccaacgatgc gccgagcgcc ttcactcggc tcctcgacat
     2281 ggaggtcgag ccgttcctca tctcttccac ggtcataggc gtgctggccc agcggctcat
     2341 tcgcaaactc tgcctgcatt gccgcgaggc ctaccagccg gaggcggcga tactcaggga
     2401 actcggactc gaccccgagg aataccgaca ccacaccttt taccgggccg tcggatgcga
     2461 gaaatgttcg cataccggct atcagggccg tatgggcatc tttgaactac tcgtcatcga
     2521 tgatgatatc cggcgtgcgg tcgtcaaccg gcaggatgcc tcggtcatca agaagaccgc
     2581 ggcggcggcc ggcatgttga ccctccgcga agacggcgcg atgaaggcgg tgaaggggat
     2641 cacctcgccc gaagagatcc tgctcaggac gcaggaggat acctagatgg ccctatacgc
     2701 ctacaggatc atggatcgtg atggtcatga gcgcaaaggg acgatagagg gatcatcgcg
     2761 taacgcggtc aaggcgaaat tccaagctga tggctggttc gtcgtcgaca tacgggaaaa
     2821 ggcctcgcga gaagggcttt cccttttcag gaccgttttt tccatgcgcc gcaagaaggt
     2881 cgcgatagag gaggtcgcct cgatgacgcg gctcctcgcc acgctccaga aggcgcgcat
     2941 cccgcttgtc gagtctatcg ccgcggtcgc ccagcagcag gacaatccgc ggctcggcga
     3001 ggtgctcatg ctcgtcaaga ccaagatgca ggagggctat agtttttcgc gcgccgcacg
     3061 cgattttccc gaggtgttca gtccgctgta ctgcaacatg atagcggcgg gagaagaaag
     3121 cggcgccacc gaagcggtcc tgttacgact cgccgatttc ctcgagtcac agatggacct
     3181 tcgcaacaag gtccgttcgg cgatgaccta ccccattatt ctcttcgtcg tcgcgatcat
     3241 ggtcgtcgcg ttgcttttca cggtggtcat ccccaagatc tccaagatgt tcgaagatgt
     3301 caacatgatc ctgccgctcc agacgcgcat cctcatctgg ataagcggca cggtcggttc
     3361 ttactggtgg ctgatattgc ttgtggccgt cgccggatat gccgggtttc accgctggaa
     3421 aaagaccccg gcaggcgagg agaaatggaa ccgccttctc atcaccgccc ccatcttcgg
     3481 acgtatcaac cggatggtgg ccatatcacg cttcgccaag accctttcca ccctcctcac
     3541 ctcgggtgtt ccgatacttg cggcacttga tattgtccgc aaggtcatcg agaaccgcgt
     3601 tatcgagaat gccgtcgcag cggcgcgaga gaacatcaag gagggtgaat ccatcgcggt
     3661 cccgttgaaa cggagcgggg aattcccccc gatagtgata cagatgatag cgataggcga
     3721 aaaatcgggt gaactggaag aaatgcttga aacactcgcc ggctcgtacg aaaaggaggt
     3781 caggtattca ctcgaacggc ttacggcgac catcaacccc atcatgatag tgttccttgc
     3841 gctagtgatc ggattcatca tattcgcggt ggtcatgccg atactcaaga taaacgaagc
     3901 ggtcggttag agaggtataa cctgtgagga ggaaagacat gttgcgcaac atcatgaagg
     3961 cgttgttccg gtcacgcgcc gccagcgcgg ggttctcgct tctggagatc atggtcgtca
     4021 tggctatcat ggcgatgatc atgggggccg tcggggtcgg gttcatgaac tatctcgaca
     4081 aggcgaaggt caaacaggcg gcgatggaca tcaagaccat ctcgaacgcg ctcgacctgt
     4141 acaaaagtga gttcggcaag tatcccgacg gactcgatgc gctcgtgcag gagaagatcc
     4201 tcaaggaaaa gaaggtcccc aaggacccgt ggaccaacga gtatgtgtac atcaaccccg
     4261 ggtcgaacaa ctccgaggga ttcgatctgt actcgttcgg caaggacgga cgtgagggag
     4321 gcggggacga catcacgaac tgggaagaag agtagcggtt cgaaggcatg aggaacggaa
     4381 ggatcgcggg accacaatca tgatgcggta cggattcacg atgatagaga tgctcgcggt
     4441 gctggccttc atggtcatca ttatgggcgt atcgataccg gcggtcacca acatatcccg
     4501 ttccgaggcg gcgtcagaat taggcatatt ccaagccttt gtcaagagaa ctttccaaca
     4561 ggcggtccgc cgcaacgaat atctccgctt ggtggtggac atgcagagcg gcgattattg
     4621 ggtggaaaag accgagaccc cctttttcct catgacggga caggagcagg agtcctacga
     4681 caaacagaac cagcgcctgc ttgaagaata tgacgagagc gagcgatcgt tcggttctct
     4741 gaaggcgggc gacgcggcga cgagccgcag tctgttcgac ctcttgggtg ctcaaggtga
     4801 cgataccact tacgataccg aggatctgta tcactgggag aatttcgtgc ccgaacggcg
     4861 caacatccgc gaattgctta agccggaata tcaaccggtc tcggatcgca ggaaggtgcc
     4921 gtcaggtctt cagtggagac aattcttctc ctatcatacc ccggaggtgc tcacgccgga
     4981 agaggcggaa gagaaggagc gcaaggcgat ggcctatgtg tatttcttcc cgcaggggag
     5041 aatatcgccg ttctttcttt ccataggcga gaaggaggga gagccgttac tccacatcgc
     5101 ggtcgatatg tacctcggta tcaggatagg gaaaggcgaa cttgacgaag aggtcaaagg
     5161 tctcgtgcaa aaaagcatag aggaagacat ggacgaggaa ggaaaatgac cgtggcgctg
     5221 tcgatgatcc ctgtcagagg agaaagcggt ttttccctgc tggaggtcac cgttgccatc
     5281 gcgatactcg cgggactgct catatcgatg gcgcgcatcg tttcgcacag ttactactac
     5341 accgaacggg tcgccgacat ctacctcggc accgagctcg cgcgtctcaa gatgcacgag
     5401 atagaggagg agataaaaaa agacggcata ccgaccgccg ataaagagga cgaaggcgat
     5461 ttcgacgaac aggcatacga agggttcaag tggcactaca gcataaaacg ggtccacatc
     5521 ccgcttcccg atgtttcggg atcggacctg tcgggggtgg agagcgggca ggcgcaggcg
     5581 gccgatctgg tgggcggcgc caagtcgatg atagaggatt tcatcaagga gaagatacgg
     5641 aaactgacgc tcgaagtgtt ctgggacgag gggaagaaag aatcggagaa ggtgaccttc
     5701 gtcatgttca ttcccgccga cacgagtgct aaggagttcc agcagggcgg cgcggcggtg
     5761 ccgggcgcgc agcagcgccc cggcggtggc cagcagaaat ggttcaacag tccgacgggc
     5821 gggggcgggc tcaaccccgc gacgcgtggc ccggcctttc gtccgggaac gacgccgttg
     5881 ccggcggggg gaccgagatg agacgccggg gcttcacgct catagaggtg ctggtcgcga
     5941 ccgctattct cgcgctcatc gtgtcgtcgg tctatttttc ttttgccaac ctcattgagg
     6001 gccggacccg tatccgtgaa cgcgccgagt cgcttcgtcg cgtctacttc gcgatagatc
     6061 tcatgcgccg cgacatacag aacgcctacc tgacgcgcaa ccgcggcacg cccgaggaga
     6121 cgcacatcac catcttcgaa ggcgaagagg acagcccggt cactcacctg acctttgcgt
     6181 cgctcaacca tgtcaagatg cgggcgggcg gcaaggagag cgaccagacc gagatagaat
     6241 attacggcga gacggtggac ggcgtgccga cgctcatgcg tcgcgagagt gcgtgggtgg
     6301 acggtttccc cgagaagggc ggcaacgcct ttcccatatt gagcgatttc cggtcgatcg
     6361 ccatagagtt ctgggacgcc gaggaccgca cctggctttc ctcgtggaac agcgagggga
     6421 ccgaccgtgc cgatacgcta ccgcccaagg tgaagataac ggcgcaggtc cgtgacgccg
     6481 acggcgacag gccggagttc ctcgtcgagt cagtggtgtc gataaagatg cagaaaccat
     6541 ttactttcta aaacatgcga cagtgcggga gaaacatcgt tgatctgttc cgtcggcccc
     6601 gcggggtggc
//
LOCUS       DAOQUE010000104         6601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12810_length_6601_cov_4.806141, whole genome shotgun sequence.
ACCESSION   DAOQUE010000104 DAOQUE010000000
VERSION     DAOQUE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12810_length_6601_cov_4.806141"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKH10_04485"
     CDS             complement(<1..654)
                     /locus_tag="PKH10_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27420.1"
                     /translation="MFRPFVGSLLLAALTFLFGGCGLINAITTSTHESETISVATVWE
                     HDHVISGTLTVNAPLTIRACANIKMENGGSIVVRDNGSIIAIGSADCDTRFTSSKTIP
                     AAGDWDAIYIYNTASAGNAFEKVTFEYGGGTYGVLWVEDNASVRVDASTFAHIKSTAV
                     MIEDGATIGSFVGNSFKSIGGNPVWTSGNIVPSLSPVLTTDLTAAVNRIHIDGTTVTE
                     "
     gene            813..2009
                     /locus_tag="PKH10_04490"
     CDS             813..2009
                     /locus_tag="PKH10_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT27421.1"
                     /translation="MMFLLSIGGGAVALFLAVALLAKKERHLAENLLSITLLLQAFDL
                     FLRGYIEGQGYRSFPFLIGLDRSLPLLYGPILYLLAASAAGQIRRLTPIHLLHLIPFI
                     FHLLLMAPFYLKNQATRIFLQERYIPGVAMLEPSPVFLFGLKALVLLCYLIAAFAAMA
                     RYERLLREFYSDIERHALSWLRLLFLVPVAQCLILCGSLLFHLLYLPWFVRINVPGGY
                     YALHLLFAALAGMQLFRSPGRWRSTVDIERLLEPEPVEGTEAKEKYQKTRLEQGLAEE
                     YRERLMHHMEETRPYTDPLLTLPQLAKQVGIPAHHLSQVLNGLLGMNFYAFVNGYRIG
                     YAKELLGKADGPANVLEVAFAAGFNSKSAFNTYFRKETGMTPTEFRRQTIGRTEGGGG
                     EKKKAQ"
     gene            complement(2104..3441)
                     /locus_tag="PKH10_04495"
     CDS             complement(2104..3441)
                     /locus_tag="PKH10_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27422.1"
                     /translation="MTKYLTILAAMVLFVACNSGSSVKSDIDTPGDDINSDNDLINTE
                     ETTGSMDNEYLNPDNNESTDDEETTDLAENEVSDSTDDDVQTDNDIAISPWVSQWLEI
                     TTGDGHTCGIKTNNRLYCWGNNDAGQLGDWTTNEKRIPTKIGDDPWIKISAGSGYDYS
                     GYTQRSHTCGIKQDNKLYCWGGNDYGQLGGGTTDDKRIPTKIGDETWVNISAGYYYTC
                     GIKQDKKLYCWGSNYCGQLGDGTTEDKYTPTKIGDEAWAHITTGLNHTCGVKQDHKLY
                     CWGGNYYGQIGDGTTDDKLTPTKIGEDTWSDISAGGHYTIGLKTDDKLYYWGNIWSEQ
                     SGYETGLLTPIKIGDDAWTNITAGYRSICGTKPDNKLYCWGYGPLGNGTYDTQSSPTK
                     IGNAMWIKLAAGGSHICAIRTNNSLYCWGDNYYGQLGDGTTDDKFVPTPIAEP"
     gene            complement(3473..3787)
                     /locus_tag="PKH10_04500"
     CDS             complement(3473..3787)
                     /locus_tag="PKH10_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNT27423.1"
                     /translation="MAMRVNRTPTHPGEMLREEFLAPLGITQSGLAQILGVSFRTINE
                     LVNCRRELSVEMALKLARFFGTTPELWLNLQNQFDIHVLAERKGRQINGIKPLVQLAP
                     TR"
     gene            complement(3793..4074)
                     /locus_tag="PKH10_04505"
     CDS             complement(3793..4074)
                     /locus_tag="PKH10_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNT27424.1"
                     /translation="MIKTFASRETELLFVNGTSRRLPSDIVRVALRKLDYLNAAATVN
                     DLRVPPGNRLEALRGDRKGRYSIRINDQFRLVFRFESGDAYDVEIVDYH"
     gene            complement(4180..5577)
                     /locus_tag="PKH10_04510"
     CDS             complement(4180..5577)
                     /locus_tag="PKH10_04510"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNT27425.1"
                     /translation="MNAERKRLLYDLPLIPVPQAGEIAATRSYPEVRVIWNGTLMEVI
                     AVRGAVSIGHAEAADIVVDESVWDEPLHELIAVRDGVATLRLTRAMNGRIYTPKSSRE
                     VAAYLDEREETLELGPGCGAVLEFGPVTVAIRMTVREKVRPAPILEQVDTAALSLFAL
                     LFIIGLLLINAIVETPPIETDFVVVPIEGKFKPIPLDEVPVEEPEPVKKIILKRDNDG
                     LHDAAQARKALGEEGSVGSKTSRVANAQGSSRKGLEDKVTRSSGIVGALTSGARAFDR
                     IFGGGGLGAGLERTLGSVTGLSGVDQFGSGGLSTRGFGTGGGGNALAIGGFHTRGKGG
                     DGSTVGYGMDKGRIGGKTLANIDAGGGQAVIMGALDRAVIDFYVRQNLARVRWCYEKE
                     LSRNAALFGKIAINFTISKSGQVSTSKVERSTMQSEPVETCVASVIKKIRFPSPKGGG
                     IVVVTYPFVFKNVVN"
     gene            complement(5593..>6601)
                     /locus_tag="PKH10_04515"
     CDS             complement(5593..>6601)
                     /locus_tag="PKH10_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017659721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HNT27426.1"
                     /translation="IVVYAGAAGLVLPPTAGSEKKKILAAIDDLRAGGSTAGAQGIEL
                     AYKTAGDIFAAEANNRVILATDGDFNVGISDDATLVKMIEEKRNRGIFLSVVGFGMGN
                     YKDAKMEKLADKGNGNYAYIDGLLEAKKVFVKQLGGTLLTIAKDVKIQIEFNPKAVAS
                     YRLIGYENRRLAKEDFNDDKKDAGEIGAGHTVTAFYEIVPVGSEELPAVDPLRYQKQA
                     DASDAPTGELALVKLRYKEPKEDASRLLTRPVPVASRRVFTEASEDVRFAAAVVGFGM
                     LLKKSPYAGKFDWKAVIAAAKGAKGVDDEGYRAEFLRLAEKAELLSETAGRSADAGQE
                     H"
BASE COUNT         1168 a         2046 c         1889 g         1498 t
ORIGIN      
        1 ctcggtgacg gtcgtgccgt cgatgtggat gcggttgacc gcggcggtga ggtcggtggt
       61 gaggacggga gagagcgacg ggacgatatt acccgaggtc cagaccgggt tgccgccgat
      121 gctcttgaag gagttgccga cgaaactgcc tatcgtcgcg ccgtcctcga tcatgaccgc
      181 cgtgctcttg atgtgggcga aggtcgaggc gtcgacgcgg accgacgcgt tgtcttccac
      241 ccacagtacg ccgtaggtgc cgccgccgta ctcgaaggtg accttctcga aggcgttgcc
      301 ggccgacgcg gtgttgtaga tgtaaatggc gtcccagtcg cccgccgccg gtatggtctt
      361 ggaggaggtg aagcgggtgt cgcagtcggc cgagccgatc gcgatgatcg agccgttgtc
      421 gcgcacgacg atggagccgc cgttctccat tttgatgttc gcgcaggccc ggatggtgag
      481 cggggcgttg acggtgagcg tgccgctgat gacatgatcg tgctcccaca cggtcgcgac
      541 gctgatggtc tcgctctcgt gggtcgaggt ggtgatggcg ttgatgagcc cgcagccgcc
      601 gaacaggaag gtcagtgcgg cgagcaggag ggaaccgacg aacggtctga acatggggaa
      661 ctcctttttt aatgacggat aagatacggg gggcactata ggaataacgg cggcgaagtc
      721 aaatttccgc cccgtcgagg ggagggtgaa aacttgcaaa tcgcgagagg gacggtaccg
      781 ttgacgaaag aagatatcca aagggtggcg gcatgatgtt ccttctttcc attggcggcg
      841 gcgcggtggc gctgttcctc gcggtcgccc tgctcgccaa gaaggaacgg catcttgccg
      901 agaatctttt gtcgatcacg ctcctgttgc aggcgttcga cctgttcctg cgcggctaca
      961 tcgaaggcca ggggtaccga tccttcccgt tcctcatcgg gctcgaccgc tcccttcctc
     1021 tcctgtacgg gccgatcctc tacctgctcg ccgcgtcggc ggccgggcag atacgccgct
     1081 tgacgccgat ccatctgttg catctgatac cgttcatctt ccacctcctg ctcatggccc
     1141 cgttctacct gaagaatcag gcgacgcgca tattcctgca ggaacgctac atcccgggcg
     1201 tcgcgatgct cgagccgtcg ccggtcttcc tgttcgggtt gaaggcgctc gtgctcctct
     1261 gttatctcat cgccgccttc gccgcgatgg cgcgctatga acggctgctc cgcgagttct
     1321 attccgacat cgagcgccac gcgctgtcat ggctccgcct gctgttcctc gtgccggtcg
     1381 cccagtgcct catcctctgc gggagtctgc tgttccacct gctctatctg ccgtggttcg
     1441 tccggatcaa tgtccccggc ggctactatg cgctccacct gctgttcgcc gcgctcgccg
     1501 gcatgcaact gttccgctcg cccggacgct ggcggtccac cgtcgacatc gagcgtctgc
     1561 tcgaaccgga accggtcgag ggaacggagg cgaaggagaa atatcaaaag acccgtctcg
     1621 aacagggact cgccgaggaa taccgcgaac ggctcatgca ccacatggaa gagacgcggc
     1681 cctacaccga tccgctcctc acgctcccgc aactggccaa gcaggtcggc ataccggccc
     1741 accatctctc gcaggtcctc aacggcctct tggggatgaa tttctacgcc ttcgtgaacg
     1801 gttatcgtat cgggtacgcc aaggaactgc tcggcaaagc cgacggcccg gccaatgtgc
     1861 tcgaggtcgc gttcgcggcg gggttcaact cgaagtcggc gttcaacacc tatttccgca
     1921 aagagaccgg catgaccccg accgagttcc ggcggcagac gatcggccga acggagggag
     1981 gcggtggaga aaagaagaag gcgcaatgat gcaagaacaa gcaagaatat cgagcggtca
     2041 gagggaggtt gcccgttaaa gagcaaggat ttttgtccag agaataaaag ggattagcct
     2101 agatcaaggt tcggcaatag gggttgggac gaatttatca tcggttgttc cgtcgccaag
     2161 ttggccataa taattgtctc cccagcagta gagactgttg tttgtcctta tcgcgcagat
     2221 atggctgcct ccggccgcaa gtttgatcca catagcgttg cctatctttg tcggggaaga
     2281 ttgagtatca tatgtcccat tgccaagagg accatatccc cagcagtaca acttgttgtc
     2341 tggttttgtc ccgcagatgg aacgatatcc ggcagttata ttggtccatg catcgtcgcc
     2401 tatttttatg ggggttaaga ggcctgtctc atagccactt tgctcagacc aaatatttcc
     2461 ccagtagtac agcttgtcgt ctgtctttag cccgatggtg taatgtcctc cggctgatat
     2521 atcggaccat gtatcttcac ctatctttgt cggggtaagc ttgtcgtcgg ttgttccatc
     2581 accaatttgg ccataataat taccgcccca gcaatagagt ttatggtcct gttttacccc
     2641 gcaggtatga ttcagtccgg ttgttatatg agcccatgcc tcgtcaccta tctttgtcgg
     2701 ggtatattta tcttcggttg ttccatcgcc cagttgacca caataattgc ttccccagca
     2761 gtagagtttt ttgtcctgtt ttatcccgca ggtgtaataa tatccggctg atatgttgac
     2821 ccatgtctcg tcacctatct ttgtcgggat gcgtttatca tcggttgttc cgccgcccag
     2881 ttggccataa tcattaccgc cccagcaata gagtttgttg tcttgtttta tcccgcaggt
     2941 gtgactcctt tgggtataac cactgtagtc ataaccactt ccagctgata ttttgatcca
     3001 tgggtcgtcg cctatctttg tcggaatgcg tttttcgttg gttgtccaat cgccaagctg
     3061 gccagcatca ttatttcccc agcagtagag tctgttgttt gtttttatcc cgcaggtatg
     3121 accatctccg gtcgttatct caagccattg agagacccat ggagaaatcg ctatatcgtt
     3181 atctgtctgc acatcatcgt cggtcgaatc ggagacttcg ttttctgcca aatccgttgt
     3241 ttcctcgtca tcggtcgact cgttattgtc cggattgagg tattcgttat ccatcgaacc
     3301 agttgtttcc tcggtgttga tcagatcgtt gtctgaatta atatcgtctc ccggcgtatc
     3361 gatgtccgat tttacacttg aaccgctgtt acaggcgaca aacagcacca tggccgcaag
     3421 aatcgtaagg tacttcgtca tgaccagtcc ctcccccgag aaggtcgttc ttctatctcg
     3481 tcggcgcgag ttgcacgagc ggtttgatgc cgtttatctg gcgtcccttg cgctcagcca
     3541 gcacatgaat gtcgaactga ttctgcaggt taagccacag ttcgggggtg gtgccgaaga
     3601 atcgcgccag tttcagcgcc atctcgaccg aaagttcgcg gcggcagttc accaactcgt
     3661 taatggtgcg gaacgaaacg ccgagtatct gcgccagtcc gctctgcgtg atgccgagcg
     3721 gcgcaaggaa ctcctcgcgc agcatctcgc ccggatgggt cggggtcctg ttcacacgca
     3781 tggccatctc cctcaatgat aatcaactat ctccacatcg tacgcgtcgc cgctctcgaa
     3841 ccggaacacc agccggaact gatcgttgat tcggatgctg taccgaccct tccggtcgcc
     3901 gcgcaacgcc tcaagccgat ttcccggcgg cacgcggagg tcgttcaccg ttgccgccgc
     3961 gttgagataa tcaagtttcc gtaacgccac cctcacaata tcggacggca accggcggct
     4021 cgtcccgttg acgaacagca gttcggtttc ccgtgacgcg aaggtcttta tcatgtttcg
     4081 agtataacgc gtggcgtgct aatgtcaagg gggggggtca aaactttatg gtcgccgacg
     4141 gaaaaaaggg agatcaaaac aagttccggc ggcaggcggt cagttcacga cattcttgaa
     4201 gacgaagggg taggtgacca cgacgatgcc gcctcccttg ggtgagggga agcgtatctt
     4261 cttgatgacc gaggcgacac aggtctccac cggctcgctc tgcatggtac tccgttcgac
     4321 cttcgaggtc gatacctgcc cgctcttcga gatggtgaag tttatcgcta tcttgccgaa
     4381 aagagccgcg ttcctgctca gttccttttc gtagcaccag cggacccgcg cgaggttctg
     4441 ccgcacatag aagtctatga cggcgcggtc gagcgccccc atgatgaccg cctgtccgcc
     4501 gcccgcgtcg atgttggcga gcgtcttgcc gcctatccgc cccttgtcca tgccgtagcc
     4561 gacggtcgag ccgtcgccac ctttcccgcg agtgtggaag ccgccgatgg cgagcgcgtt
     4621 gccgccgccg ccggtgccga agccgcgtgt gctgagcccg cccgagccga actgatcaac
     4681 tcccgacagc ccggtcaccg acccgagcgt ccgttcgagc cccgcgccga gtccgccgcc
     4741 gccgaaaata cggtcgaacg cccgcgcgcc actggtgagt gcgccgacga tgcccgaact
     4801 gcgggtgacc ttgtcctcga gccccttgcg cgacgagccc tgcgcgttgg cgaccctgct
     4861 cgtcttcgat ccgacgcttc cctcctcgcc gagcgccttg cgggcctgcg ccgcgtcatg
     4921 gagcccgtcg ttgtcgcgct tgaggatgat ctttttcacc ggctccggtt cctcgaccgg
     4981 cacctcgtcg agcggtatcg gcttgaactt cccttcgatc ggcaccacga cgaaatcggt
     5041 ctcgatgggt ggcgtctcga cgatggcgtt gatgaggagc aggccgatga tgaacaggag
     5101 cgcgaagagc gacagcgcgg cggtgtccac ctgttcgagt atcggcgccg gccgcacctt
     5161 ttcgcgtacc gtcatgcgga tggccacggt gaccggcccg aactcgagca ccgcgccgca
     5221 ccccggtccc agttcgagcg tctcttcccg ctcatcgaga tacgccgcca cctcgcgaga
     5281 acttttcggc gtgtagatcc gtccgttcat ggcgcgggtc agccggagcg tcgccacccc
     5341 gtcgcgcacc gcgatgagtt cgtggagcgg ctcgtcccac accgattcgt ccaccacgat
     5401 gtcggccgcc tcggcgtgac cgatgctcac cgcgccgcgc accgcgatga cctccatcag
     5461 cgtcccgttc cagatgaccc ggacctccgg gtaactccgc gtcgccgcaa tctctcccgc
     5521 ctgcgggacc ggtatcaggg gaagatcgta cagcagtcgt ttcctttcag cgttcatgac
     5581 accctccttc ggttagtgtt cctgtccggc atcggccgac cgccccgccg tttccgaaag
     5641 cagttccgcc ttctcggcca gacgcaggaa ctcggcccga tacccttcgt cgtcaacccc
     5701 tttcgccccc ttcgccgccg cgatgaccgc cttccagtcg aacttcccgg catagggcga
     5761 ctttttcagc agcatcccga acccgaccac cgccgccgcg aaccggacat cctccgacgc
     5821 ttcggtaaag acgcggcgcg acgcgaccgg caccgggcgg gtcagcagtc tgctcgcatc
     5881 ttctttcggt tccttgtagc gcagtttcac gagcgcgagt tcgcccgtcg gggcgtcgct
     5941 cgcgtcggcc tgtttctgat accggagcgg atcgaccgcc ggcagttcct ccgaaccgac
     6001 cgggacgatc tcgtagaacg cggtgacggt gtggcccgcg ccgatctcgc ccgcgtcctt
     6061 cttgtcgtcg ttgaaatcct ctttggccag acggcggttc tcgtagccga tgagccggta
     6121 ggaggcgacc gccttggggt tgaactctat ctgtatcttg acatccttcg cgatggtgag
     6181 cagcgtgccg ccgagttgct tcacgaagac cttcttcgct tccagcaggc cgtcgatgta
     6241 ggcgtagttg ccgttaccct tgtcggcgag tttctccatc ttggcgtcct tgtagttccc
     6301 catcccgaag ccgacgaccg agaggaatat tccccggttg cgcttttcct cgatcatctt
     6361 caccagcgtc gcgtcgtcgg agataccgac attgaaatcg ccgtcggtcg cgaggatgac
     6421 gcggttgttc gcctccgccg cgaagatgtc ccccgctgtc ttgtaggcca gttctatccc
     6481 ctgcgccccc gccgtcgaac cgcccgcccg cagatcgtcg atggcggcga gtatcttttt
     6541 cttctcgctt cccgccgtcg gcgggaggac gagccccgcg gccccggcgt agacgacgat
     6601 c
//
LOCUS       DAOQUE010000105         6595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12824_length_6595_cov_3.696789, whole genome shotgun sequence.
ACCESSION   DAOQUE010000105 DAOQUE010000000
VERSION     DAOQUE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12824_length_6595_cov_3.696789"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1326
                     /locus_tag="PKH10_04520"
     CDS             1..1326
                     /locus_tag="PKH10_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HNT27427.1"
                     /translation="MLNDISGIIWGWPLIALLFGTHLFLTVRLRFIQRHIGTAIKLSF
                     SRKSEGSGEISHFGALTTALAATIGTGNIVGVATAVAVGGPGAVLWMWLTGIFGIATK
                     YGEAVLAVKYRRLLPDGSVAGGPMYALEDGLKSKWLGILFAAFTAIAAFGIGNMVQAN
                     SIAGLMGDTFGVPGWATGLVLTIATALVIIGGVRSIAKVCEKLIPFMVVVYFTGCLIL
                     LALGWRTIPDSLWLIVSSAFGGHAALGGFVGAALKETVRYGIARGLFSNEAGLGSAPI
                     VAAAAQTKNPVRQALVSSTGTFWDTVVVCLITGLVLVNSGRWTEGLNGAALTHAAFSQ
                     LGAFGPALLTFGLLTFVFSTILGWSYYGEKAIEYLFGHASVRPYRWLWVAMVMLGSTL
                     SLGAVWTFADISNGLMALPNLIALLLLNGVIVAETRRYLWTPGKLDSED"
     gene            1408..2472
                     /gene="ugpC"
                     /locus_tag="PKH10_04525"
     CDS             1408..2472
                     /gene="ugpC"
                     /locus_tag="PKH10_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNT27428.1"
                     /translation="MATVTFEKVVKRYSEQAVILRGIDLEVHDGEFLVLVGPSGCGKS
                     TLLRTLAGLERIEEGAIRIGDRPVGDLPPKDRDIAMVFQSYALYPHMTVRENMAFGLT
                     VRRMPAAEIARRVDEAAALLGITHLLDRLPKEMSGGQRQRVAMGRAIVRSPQVFLFDE
                     PLSNLDAALRTQMRIELKHLHRRLGATIIYVTHDQIEAMTLADRIVVLDKGVIQQIGT
                     PAELYDRPANTFVARFIGTPAMNLFDIEPRDGAIFGGAVRLPGTARRIVAGIRPSDLT
                     VATAPGGIRLDGTVDLVEALGATAVAHFRLDADRRITAELPAVAARGLAAGATVTLTA
                     APDRVHLFDGETGLRIADRA"
     gene            2469..4664
                     /locus_tag="PKH10_04530"
     CDS             2469..4664
                     /locus_tag="PKH10_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT27429.1"
                     /translation="MKRLTLFTLFLFLCIATLDAAPREITVWHSYRGTEETALAGLAE
                     EFNALHHDDIRVTLLAVPYDAFANKITSAVPRGNGPDAFIYAHERIGDWAASGVIEPL
                     GPYYTEHLGTRMLPVTIEAMRSGDKLWGIPLAFKSLVLFYRTDLIETPPVTTDELLAV
                     AERFTDRAANRYGIAFEAASAYFAAPFLTGFGGGFCLDEVASDTPCLDTPENAAALSF
                     IAEIVRERNVAPDEATGALVTHLFAEGRVPMAISGPWLMGELPTGLPYAVATLPVVSA
                     TGLPMRPLLTVEAYLIAKHRRTGIAAARAFGEFLSSEAAARQRALTGRQSVATLFAYD
                     SPAVRNDATLMLLKRQAEQALPMSNRPEMRMVWEPLATALRKVLRGADAPQHALAEAQ
                     RHYVLFTRPPAKAQSPAIFLATLLALAVVGFIYLWWRFRRERVIATMRRAPTPYLCLL
                     PAALSMFILVFIPFTVGTAVSFFAHRAGEWRFVGFANFVSILSAKDFPVTDPLNFYFT
                     LAVTVGWTAVNVALHVAIGLALAMLLRDQWLRLRGVYRILLIVPWAVPNYITALIWKG
                     MFNRQFGAVNGLLDLFGVEPVSWFSSFWTAFAANVATNTWLGFPFMMVTALGALQAVP
                     RELEDAAAVDGAGPLDRFRHVILPLLKPALLPAVILGSVWTFNMFNIIFLVSGGDPDG
                     ATDILISEAYKWAFQRQEQYGYAAAYATLIFIVLLLYSRFTEQRAGGAA"
     gene            4661..5509
                     /locus_tag="PKH10_04535"
     CDS             4661..5509
                     /locus_tag="PKH10_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNT27430.1"
                     /translation="MKDRSLTRVLLTHAALVAACVVTLYPVLWVVKMALTPAQGFSLS
                     VSPIPDAVSLQNFREVIGTVGPQGGWLFGRQFLNSVVISLATTVVGVTLATTAGYAFS
                     RFVFPGRDLGMKLFLVSQMFPGVVMAIPLYILLDNLHLLNSMTGLVLVYATTAIPFCV
                     WMLKGYFDTIPRDLEEAALIDGASQWTIFTRIVLPLARPAIVVTALFSFMTAWNEFIL
                     AATFLSSEQAFTLPVALQRYVGSFNTEWGHFAAGAIIVSLPVMLLFFFLQKHLIGGLT
                     AGGVKG"
     gene            5584..>6595
                     /locus_tag="PKH10_04540"
     CDS             5584..>6595
                     /locus_tag="PKH10_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27431.1"
                     /translation="MEVLMRNISSLAIAMALLFVACGEEPEKTPTDSNAATDDMSVSD
                     ADTAVPGDDFFATEATNSFRMEFKGLINAYDDMVASKQVKGIGSFEYSFDGEEYTPKG
                     DPEAYLSTIPTDYEDPALAGKEYLRMQLYAGPLEQGTSEAGNAWYTYDYINIGIPLDR
                     FTAAKSTAENIFALDDVTWANLMTVTLTARTDGKYFYRYCYTALPQRDPTDGKWFVDH
                     RNNESFAAGEDLVLWANITMGEKQAVTAENEETLCLYQSQTTELTKVEYEAEIAKSGT
                     EFGCEIPEKYTTAFADDRLLFTFIGEISDIANNETRLAYADYDLLIGGVGEPCFVVGD
                     VRY"
BASE COUNT         1124 a         2303 c         1956 g         1212 t
ORIGIN      
        1 atactgaacg acatcagcgg catcatctgg ggctggccgc tcatcgccct gctattcggc
       61 acccacctgt tcctcaccgt caggctccgc ttcatccagc gtcatatcgg cacggcgata
      121 aaactctcgt tcagcaggaa aagcgaaggt tcgggagaga tcagccattt cggcgcgctc
      181 accaccgcgc tcgccgccac catcggcacc ggcaacattg tcggcgtcgc gaccgccgtc
      241 gcggtgggcg gtcccggcgc ggtgctctgg atgtggctca ccggcatctt cggcatcgcc
      301 accaagtacg gcgaggcggt ccttgcggtg aaataccggc ggctcctccc cgacggctcg
      361 gtcgccggcg gcccgatgta cgcactggag gacgggctca agtcgaaatg gctcgggatc
      421 ctcttcgcgg ccttcaccgc gatcgccgcg ttcggcatcg gcaacatggt acaggcgaac
      481 tccatcgcgg gactcatggg cgacaccttc ggtgttccgg gctgggcgac cggtctcgta
      541 ctcaccatcg caacggcact cgtcatcatc ggcggggtcc gctccatcgc gaaggtctgc
      601 gagaaactga taccgttcat ggtggtggtc tatttcaccg gctgtctgat actgctcgcc
      661 ctcggctggc ggaccatccc cgattcgctg tggctcatcg taagttcggc cttcggcggt
      721 cacgcggcgc tcggcggctt cgtcggcgcg gcgctgaaag agacggtccg ctacggcatc
      781 gccagaggcc tcttctccaa cgaggcggga ctcggcagcg cccccatcgt cgcggcggcg
      841 gcacagacga agaacccggt gcggcaggcg ctcgtctcat cgaccggcac cttctgggac
      901 accgtggtgg tctgcctcat caccgggctc gtgctcgtca attcgggtcg ctggaccgaa
      961 gggctgaacg gcgcggcgct cacccatgcc gccttctcgc aactcggcgc gttcggcccg
     1021 gcgcttctca ccttcgggct cctcaccttc gtcttttcga cgatactcgg ctggtcctac
     1081 tacggcgaga aggcgatcga atatcttttc ggccacgcaa gcgtccgtcc ctaccgctgg
     1141 ctctgggtgg ccatggtgat gctcggatcg acgctctcgc tcggcgcggt atggaccttc
     1201 gccgacatct ccaacggact gatggcgctc cccaacctca tcgcgctgct tctgctgaac
     1261 ggcgtcatcg tcgccgagac gcgccgatat ctctggaccc ccggcaaact cgacagcgag
     1321 gattgatccg gtctcttttc ctttgacttg tctgcgtgga attgatacta tcctcgcaac
     1381 tgacatacaa cgaacgggag tactgcggtg gcgacggtaa cattcgagaa ggtcgttaag
     1441 aggtactcgg agcaggcggt catcctcagg ggcatcgatc tcgaggtgca cgacggcgag
     1501 ttcctcgtgc tcgtcggccc gtcggggtgc ggcaaatcga cgctcctgcg gacgctcgcc
     1561 ggactcgaac ggatagagga gggcgccata cgcatcggcg accgcccggt gggcgacctg
     1621 ccgcccaagg accgcgacat cgcgatggtg ttccagtcct acgcgctcta tccccacatg
     1681 acggtccgcg agaacatggc gttcggactg acggtccgca ggatgcccgc cgccgagata
     1741 gcgcgccggg tcgacgaggc ggccgccctg ctcggcatca cccacctgct cgaccggctt
     1801 cccaaggaga tgagcggcgg ccagcgccag cgtgtcgcga tgggccgcgc catcgtccga
     1861 agcccgcagg tgttcctgtt cgacgaaccg ctctcgaacc tcgacgccgc cctgcggacc
     1921 cagatgcgga tagaactcaa gcacctgcac cggcgactcg gcgccaccat catctatgtc
     1981 acccacgacc agatagaggc gatgacgctc gccgaccgca tcgtggtgct cgataaaggg
     2041 gtcatccaac agatcggcac gccggccgaa ctgtacgacc gtcccgctaa caccttcgtc
     2101 gcccgcttca tcggcacccc cgccatgaac ctgttcgaca tcgagccgcg ggacggggcg
     2161 atattcggcg gcgcggtgcg cctgccggga acggcccgcc ggatcgtcgc ggggatacgc
     2221 ccctccgacc tcaccgtcgc gacggcgccg ggcggcatcc gtcttgacgg cacggtcgat
     2281 ctcgtcgagg cgctcggcgc gaccgccgtc gcccacttcc ggctcgacgc cgaccgccgg
     2341 atcacggcgg aacttcccgc cgtcgccgcg cgaggacttg cggccggcgc gaccgtgacg
     2401 ctcaccgccg cacccgaccg ggtacatctt ttcgacggcg agaccggact gaggatagcg
     2461 gacagggcat gaaacgccta acccttttca cgctctttct tttcctgtgc atcgccacac
     2521 tggatgccgc cccgcgcgag ataacggtgt ggcattccta ccgcgggacc gaagagacgg
     2581 cgctcgccgg gctcgccgag gaattcaacg ccctgcacca cgacgacatc cgcgtcacgc
     2641 tcctcgcggt cccctacgac gccttcgcga acaagataac gagcgccgtg ccgcgcggca
     2701 acggccccga cgccttcatc tacgcccacg aacgaattgg cgactgggcg gcgtccggcg
     2761 tcatcgagcc gctcgggccc tactacaccg aacacctcgg gacccggatg ctaccggtga
     2821 ccatcgaggc gatgagaagc ggcgacaaac tctggggcat accgctcgcc ttcaagagtc
     2881 tcgtcctctt ttaccgcacc gacctcatcg agacgcctcc cgtgacgacc gacgaactcc
     2941 tcgcggtcgc cgaacgattc accgaccgcg ccgccaaccg ctacggcatc gccttcgagg
     3001 ccgcgtccgc ctacttcgcc gcccccttcc tgaccggttt cggcggcggg ttctgccttg
     3061 acgaggtcgc gagcgatacc ccctgcctcg atacccccga gaacgccgcg gcgctctcct
     3121 tcatcgccga gatcgtccgg gaacggaatg tggcgcccga cgaggcgacc ggcgcgcttg
     3181 tcacgcacct gttcgccgag gggcgcgtcc cgatggctat aagcggcccg tggctcatgg
     3241 gtgaacttcc gaccgggctc ccctacgccg tggcgaccct gcccgtcgtc agcgcgacgg
     3301 gactcccgat gcgaccgctc ctgacggtcg aggcctatct catcgccaag catcgccgca
     3361 ccggcatcgc ggccgcccgc gctttcggcg aattcctttc gtccgaagcg gcggcgcgac
     3421 agcgggcgct caccggccgc cagtcggtcg cgacgctgtt cgcctacgat tcaccggcgg
     3481 tccgtaacga cgccacgctc atgctcctca agcggcaggc cgaacaggcg ctcccgatga
     3541 gtaaccgccc cgagatgcgg atggtgtggg aaccgctcgc gaccgccctg cgtaaagtac
     3601 tgcgcggcgc cgacgcgcca caacacgcgc tcgccgaggc ccagcggcac tacgtgctct
     3661 tcacaagacc cccggcgaag gcccagagcc ccgcgatctt ccttgcgacg ctcttagcgc
     3721 tcgcggtcgt cggcttcatc tatctctggt ggcgcttccg acgcgaacgg gtcatcgcga
     3781 cgatgcgccg ggcaccgacc ccctacctct gtctcctgcc cgccgccctt tcgatgttca
     3841 tcctcgtctt catcccgttc accgtcggca ccgccgtctc cttcttcgcc caccgggcgg
     3901 gcgagtggcg cttcgttggg ttcgcgaact tcgtttcgat actttccgcc aaggactttc
     3961 cggtgaccga cccgctcaat ttctacttca cgctcgccgt gacggtcggc tggaccgccg
     4021 tcaatgtcgc actccatgtc gccatcggac tcgcgctcgc gatgcttctg cgcgaccagt
     4081 ggctgagact gcgcggcgtc taccggatac tgctcatcgt cccgtgggcc gtcccgaatt
     4141 acatcacggc gctcatctgg aaagggatgt tcaacaggca gttcggcgcg gtgaatggcc
     4201 tgctcgacct gttcggcgtc gagccggtat cctggttctc ctcgttctgg accgccttcg
     4261 ccgccaatgt cgcgaccaac acctggctcg gcttcccctt catgatggtg acggcgctcg
     4321 gcgccctgca ggcggtgccg cgcgaactcg aggacgccgc ggcggtcgac ggcgcgggac
     4381 cgctcgaccg gttccggcat gtgatactgc ccttgctgaa accggcgctt ctcccggcgg
     4441 tcattctcgg ttcggtctgg acattcaaca tgttcaacat catcttcctc gtgtcgggcg
     4501 gcgaccccga cggagcgacc gacatcctca tcagtgaggc ctacaaatgg gccttccagc
     4561 gacaggaaca gtacggctac gcggcggcct acgcgacact gatattcatc gtgctcctgc
     4621 tttactcgag atttaccgaa cagcgcgcgg gaggtgcggc atgaaggacc ggtcgttgac
     4681 ccgcgtactg ctgacccacg ccgcgctcgt cgccgcctgc gtcgtcacgc tctacccggt
     4741 gctctgggtg gtcaagatgg cgctcacccc ggcgcagggt ttctcccttt cggtcagccc
     4801 gatacccgac gccgtatcgt tacagaactt ccgcgaggtg atcggcacgg tcggaccgca
     4861 gggcgggtgg ctcttcggac ggcagttcct gaacagcgtc gtcatatcgc tcgccaccac
     4921 cgtcgtgggc gtcacgcttg cgacgaccgc cggatacgcc ttctcccggt tcgtgttccc
     4981 gggccgcgat ctcggcatga aactgttcct cgtttcgcag atgttccccg gcgtcgtcat
     5041 ggcgataccg ctctatatcc tgctcgacaa cctgcatctt ctcaactcga tgaccggcct
     5101 cgtgctcgtc tacgcgacca ccgcgatacc gttctgcgtc tggatgctca agggctactt
     5161 cgacacgata cccagggacc tcgaggaggc ggcgctcatc gacggcgcat cgcagtggac
     5221 gatattcacc cgcatcgtac tgccgctggc ccgcccggcg atagtggtca ccgcgctctt
     5281 ctcgttcatg acggcgtgga acgaattcat cctcgccgcg accttcctca gttccgagca
     5341 ggcgttcacg ctgccggtcg cgctccaacg ctatgtcggc agtttcaaca ccgagtgggg
     5401 ccatttcgcc gccggggcca tcatcgtgtc gctcccggtg atgctcctgt tcttcttcct
     5461 ccagaagcac cttatcggag gtctcaccgc cggcggcgtg aaggggtgaa cacctagttg
     5521 tccgcattca ctaattattc cattcaggac catgctttgc tgaaatgctt atgtatctat
     5581 ctgatggagg ttctcatgcg caacatctct tctctggcga tagcgatggc gctcctgttc
     5641 gtcgcgtgcg gcgaggagcc ggaaaaaaca ccgaccgaca gcaacgccgc cacggatgat
     5701 atgtccgtga gcgacgccga caccgccgtc ccgggcgacg atttcttcgc gaccgaggcg
     5761 acgaactcgt ttcgcatgga gttcaaggga ctcatcaacg cctacgacga catggtcgcg
     5821 agcaagcagg tgaaggggat cggcagtttc gagtactcgt tcgacggcga ggaatacacg
     5881 ccaaaaggcg accccgaggc ctacctcagt accattccca ccgactacga ggaccccgcg
     5941 ctggcgggca aggagtacct gcggatgcaa ctctacgccg ggccgctcga acaagggacg
     6001 agcgaagcgg gaaatgcttg gtacacctac gattacatca acatcggtat cccgctcgac
     6061 cgcttcacgg cggcgaaaag cacagcggag aacatcttcg cgctcgacga tgtcacctgg
     6121 gccaacctca tgacggtcac cctcacggcg cgcaccgacg gcaagtattt ctaccgttac
     6181 tgctacaccg cgcttcccca gcgcgacccg accgacggaa agtggttcgt cgatcaccgc
     6241 aataacgaga gtttcgcggc gggcgaggac ctcgtcctct gggcgaacat cacgatgggg
     6301 gaaaagcagg cggtgacggc cgagaacgaa gagacgctct gcctctacca gtcgcagacg
     6361 acggaactca ccaaggtgga gtacgaggcc gagatcgcga agagcggcac cgagttcggc
     6421 tgcgagatac cggaaaagta cacgaccgcc ttcgccgacg accgtctcct gttcaccttt
     6481 atcggcgaga tatcggacat cgccaacaac gaaacacggc tcgcctacgc cgactatgac
     6541 ctgctcatcg gcggcgtagg cgagccgtgt ttcgttgttg gcgatgtccg atatc
//
LOCUS       DAOQUE010000106         6582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_12870_length_6582_cov_3.967213, whole genome shotgun sequence.
ACCESSION   DAOQUE010000106 DAOQUE010000000
VERSION     DAOQUE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12870_length_6582_cov_3.967213"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..865)
                     /gene="fabG"
                     /locus_tag="PKH10_04545"
     CDS             complement(104..865)
                     /gene="fabG"
                     /locus_tag="PKH10_04545"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNT27432.1"
                     /translation="MLLQGKTALVTGASRGIGEAIACAFAREGADIVVHCYAHKSDPE
                     KNLRAEAETLAAKIRDMGRKAYLCGFDISDREMADTELKKLLAEVTPNILINNAGITA
                     DNLAMRMKPEEWERVININLSGAFWVTQALMRPLMKNGGCVVNIASVIGQMGNTGQAN
                     YAASKAGLIGMTKSLARELASKKVRVNAIAPGFIETAMTHAMTEEAKKALMDIIPLKE
                     TGKPEDIANAALFLCSDMARYITGEVLKVNGGMYM"
     gene            complement(959..1411)
                     /locus_tag="PKH10_04550"
     CDS             complement(959..1411)
                     /locus_tag="PKH10_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27433.1"
                     /translation="MRILAFLCSMMLYGPLIAEAPAPAVPAKPAPAPEKYSLTIVQYE
                     VSATPCAAENAMMGTITKKAGYACAAQKGRITINLTGAEAQKVTDFMNKEITVAVEGK
                     KDNYLLVRVKIPGEMDAVFMPVIGGSFVAGYDLDEKKTKGVLLLIYVV"
     gene            complement(1411..1818)
                     /locus_tag="PKH10_04555"
     CDS             complement(1411..1818)
                     /locus_tag="PKH10_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27434.1"
                     /translation="MSDLKERLAAQDREFAPLTSLLKRGFETDATLAPEVDIEAIIAG
                     TLKKLAPKRRRRLLIRFAFATTATALLFALIVPLYGPPNRPDAVPRLSFEDAERMLNT
                     NSLKEVTILYQSGRAEIETDAPVPMVWVRKGEN"
     gene            complement(1815..2315)
                     /locus_tag="PKH10_04560"
     CDS             complement(1815..2315)
                     /locus_tag="PKH10_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT27435.1"
                     /translation="MDRTTLEKLVRETRGWIFATAYHILRDRASAEDVMQETYLALYT
                     HYDTLDSKDAVYSWLKTVAINKALNAAKRNIFVELEDFVAEKIFSTPDGGERSLYADQ
                     MMERLQEGLDKLSPDHRAILLMREIEGLSYEDIAAKMKCSIGTVMSRLFYARQNIRKF
                     LERKNT"
     gene            complement(2374..3201)
                     /locus_tag="PKH10_04565"
     CDS             complement(2374..3201)
                     /locus_tag="PKH10_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27436.1"
                     /translation="MGTYFKVYHKRSRELVATFNEKQIVDFCISNKESIKKYIFETGG
                     QKDSMEWEVFALKYAPLFVQKAKEREEKERQVSDSVAIEEPSKLPWLLLAGVAGLMVA
                     IVAGALVYYYMTRDDAVRRVHDLSAPTEETPKPVEPASAAAPILPAKRDPAPGDTAFT
                     GKAPDDKTLSAAWGKVKKGLTASLSPADIKDEMDKYLPALETCFKERAQAGDRNLRGT
                     VNMKIRVAGNGAVLDVLFTDDKYRSTLFSDCIGNAIKGRSFRPFKSKEQIFTYYYEL"
     gene            complement(3218..4438)
                     /locus_tag="PKH10_04570"
     CDS             complement(3218..4438)
                     /locus_tag="PKH10_04570"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNT27437.1"
                     /translation="MGKFSSLLQKNAEEGNRFFFVFTEGKYKGGEFTVSRDAQIIIGR
                     DISCDVAIIDAKVSRKHARIWSNNGNTHIEDSNSTNGSLLNGKPLKAGEPIRLSPGDE
                     ITIGESTFTFTGAKGTDAKAEIRKPVASATPAQPAPQEIGDRPDAIPLPKRKADPATA
                     APAALDAALDSLTNDDERSIPRGVNVAKVSLEKRDLKAAMAQTRFDGSLHAKEGKLSK
                     IEPIELLKVLLQSSTVGILQVKATEPFAEKIDIRLGEKGVTGATSLTNKLFAEEKALT
                     RFLLAKDGDYHLKESEEPRKENMNQAIFDIFTEISSQKPLLTRYRKIASGKGLRFLIP
                     MTGKLSALNKKELDTVQFMANAVEVVAYLNMFPEHDDFILLSEILKYIDLGIIFGDNN
                     EEGGLPTPRDIMDV"
     gene            complement(4529..5233)
                     /locus_tag="PKH10_04575"
     CDS             complement(4529..5233)
                     /locus_tag="PKH10_04575"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNT27438.1"
                     /translation="MDIPILFEDNHLIAVDKPRGMPTQADRSDDPDLLTTVKEHLKER
                     YRKPGAAYLGMVHRLDRPVGGVVLFAKTSKAASRLAEQLRAGLFHKTYLAVVRGGPAP
                     SEARLEHHLLKDPSRNLVLVSAPGTPGSKRAILTYRVLATHGDHSLLSVELLTGRSHQ
                     IRVQLATVGHPIIGDEKYGPKSGAEHSLLALHAVSISFDHPVKHEPMRVSSPVPTEDR
                     WAPFRGTIDAHLLQGS"
     gene            complement(5237..>6582)
                     /locus_tag="PKH10_04580"
     CDS             complement(5237..>6582)
                     /locus_tag="PKH10_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HNT27439.1"
                     /translation="AGGTTVRVLILDSDAAGRFWALRSRGHDLILVGPDRAESASDGT
                     LRMEYDERQGSLALITPVGMTIPELSIGTPRDETTGLSRWRLPAWSAPLPVDPTLLGI
                     GALLEDGAAAMPGYDDSDWITWTGEPEPLEEFDILSGHAWYRATIDLPGTPTEGRLYI
                     EHASDIVGIYVNGVYVTTVSPVGTEIDSDGSERYRFADIAPLLHAGENLIAFRVEIWG
                     HGSFMWPRGKMAGFPGQMPSLGFDSLKGLWGSAYFRGKVNGSTATYDLASWRLRAATD
                     GELDSWHDTDSNELVWPKGERPATLPKGGMVWYRVPFTTTAILRDGFDAPPALVLKGR
                     NAKATIWINGILAGRWLSDEEWLCRGTWTRCLRSMWSVNEPDAIPVAPGLLFTNDSSN
                     TLTILFEDTSADTDTTGGTIEELFFGYAAEELTPDGDRSALRPLPRRELIVHLTD"
BASE COUNT         1144 a         1872 c         2074 g         1492 t
ORIGIN      
        1 gccggcgtcg gccgaaagtt tgttcgccgc gagttcgatg accttcttca tgatctcctg
       61 cttttccatg gtgcttcctc catactggtt acattagggt ttgctacata tacatgccgc
      121 cattgacctt gagcacttcg ccggtgatgt accgggccat gtcggaacac aggaacagcg
      181 cggcgttcgc gatgtcctcc ggtttccccg tttccttgag cgggatgatg tccatcagcg
      241 ccttcttggc ctcctcggtc atcgcatgcg tcatcgccgt ctcgatgaag ccgggggcga
      301 tggcgttgac acggaccttt ttcgatgcca gttcgcgggc gagcgacttg gtcatcccga
      361 tgagccccgc cttcgacgcc gcgtagttcg cctgtccggt gttccccatc tggccgatga
      421 ccgaggcgat gttgacgacg caaccaccgt tcttcatcaa gggccgcatg agcgcctgcg
      481 tcacccagaa cgcgcccgag agatttatgt tgatgacccg ttcccattcc tcgggcttca
      541 tcctcatcgc gaggttgtcg gcggtgatgc cggcgttgtt aatgaggatg ttcggcgtca
      601 cctcggcgag cagtttcttg agttcggtgt cggccatctc gcggtcggag atgtcaaagc
      661 cgcaaagata ggccttcctg cccatgtcgc gtatcttcgc ggcgagcgtc tcggcctcgg
      721 cgcgcaggtt cttctcgggg tcgctcttgt gggcgtagca gtggacgacg atatcggccc
      781 cttcgcgtgc gaaagcgcac gctatcgcct cgccgatgcc acgcgatgcg ccggttacga
      841 gagccgtttt cccttgcaat aacataatgt tatcctcctc aaagggtcag agaactgaca
      901 tgtcagtcca tctttattgc ataactaacg ggaagtcaac aaaattccag aaaaaaactc
      961 aaacgacata tatcagcagg agcacccctt tcgtcttctt ttcgtcgaga tcgtagccgg
     1021 cgacgaacga gccgccgatg accggcatga agaccgcgtc catctcgccc ggtatcttca
     1081 cgcggacgag aaggtagttg tcctttttcc cctccacggc gaccgttatc tccttgttca
     1141 tgaagtcggt caccttctgt gcctcggcgc cggtgagatt gatggtgatg cgtcccttct
     1201 gggccgcgca ggcatagccc gccttcttgg tgatggtccc catcatcgcg ttctcggcgg
     1261 cgcagggggt tgccgacacc tcgtattgca cgatggtgag cgaatacttc tcgggggccg
     1321 gggccggttt agccggaacg gcgggagcgg gcgcttctgc gatcaaaggg ccgtagagca
     1381 tcatggaaca aaggaacgcg aggatacgca tcagttctct ccctttctca cccagaccat
     1441 cggcaccggc gcatcggtct cgatctcggc gcggcccgat tggtagagta tggtcacttc
     1501 cttgagactg ttggtgttga gcatgcgctc ggcatcctcg aacgagaggc gtggcaccgc
     1561 gtcgggacgg ttcggcggcc catagagtgg aacgatgagg gcaaacaaga gggcggtcgc
     1621 tgtcgtcgcg aaggcgaacc gtatgagcag gcgcctgcgc cgtttcggtg cgagtttttt
     1681 cagcgttccg gcgatgatcg cctcgatgtc tacctcgggg gcgagcgttg cgtcggtctc
     1741 gaatccccgt ttcagcaggg aggtaagggg ggcgaactcg cgatcctgcg ccgccagtct
     1801 ttctttcagg tcgctcatgt atttttccgc tccagaaatt tccgtatgtt ctgtcgggcg
     1861 tagaacaggc gcgacattac ggtgccgata gaacacttca tcttcgcggc gatatcctcg
     1921 taggaaagtc cctctatctc gcgcatcagc aggatggcgc ggtggtcggg cgatagtttg
     1981 tcgagtccct cctgcaggcg ttccatcatc tggtcggcat acagcgaccg ttcgccgccg
     2041 tcgggggtcg agaatatctt ttcggcgacg aaatcctcga gttcgacgaa gatattccgt
     2101 ttggcggcgt tcagcgcctt gttgatggcg accgttttga gccaactgta gaccgcgtcc
     2161 ttgctgtcga gggtgtcata atgggtgtag agtgcgagat aggtctcctg catcacatcc
     2221 tcagcggagg ctcggtcgcg caggatgtga taggcggtgg cgaatatcca tccacgcgtc
     2281 tccctgacga gtttctcaag tgtcgttctg tccacgcaat gccccgtacc gacacctgtg
     2341 gcggtgtttt attcagagaa tatcaaagga tatctataat tcgtagtaat aggtgaagat
     2401 ctgttccttg ctcttgaacg ggcggaaact ccgccccttt atggcattcc cgatacagtc
     2461 ggagaacagg gtcgatcggt atttgtcgtc ggtgaagagg acatcgagca ccgcgccgtt
     2521 gcccgccacc cgtatcttca tgttgacggt gccgcgcagg ttgcggtcgc cggcctgtgc
     2581 ccgctccttg aagcatgtct cgagagcggg aaggtatttg tccatctcat cttttatgtc
     2641 cgctggggag agggaggcgg tcagcccctt tttgaccttg ccccatgcgg cggaaagtgt
     2701 cttgtcgtcc ggcgctttgc cggtaaatgc ggtatcgccg ggggctgggt cccgttttgc
     2761 cggcaggatg ggcgcggcgg ccgacgccgg ctcgaccggt ttaggggtct cttcggtcgg
     2821 cgcggaaaga tcgtggacgc gacggaccgc gtcgtcgcgg gtcatgtagt aatagacgag
     2881 cgcccccgct acgatggcga ccatgagtcc cgcgacgccg gcaagtagca accacggtaa
     2941 tttgctcggc tcctcgatcg cgacgctgtc ggaaacctgt cgctccttct cctcgcgttc
     3001 cttcgccttc tgaacgaaca ggggagcgta cttcagcgcg aagacctccc attccatgct
     3061 gtccttctgg ccgccggtct cgaagatgta ttttttgatt gattccttgt tgcttatgca
     3121 gaaatcgacg atctgcttct cgttgaaggt ggccaccaat tcccgggaac gcttgtgata
     3181 gaccttgaaa taagttccca cggtcgctcc tccttattta gacatccatg atgtcgcggg
     3241 gcgtcggcag gccgccttcc tcgttgttgt cgccgaagat gatcccgaga tcgatgtatt
     3301 tgagtatctc ggagagcagg atgaaatcgt cgtgttcggg gaacatgttg aggtaggcga
     3361 cgacctctac cgcgttggcc atgaactgca cggtgtcgag ctccttcttg ttcagcgcgg
     3421 agagtttgcc ggtcatcggt atcaggaaac ggagcccttt accggacgct atcttgcggt
     3481 accgcgtcag gaggggcttt tgcgaggata tctcagtgaa gatgtcgaat atcgcctggt
     3541 tcatgttctc cttgcgcggt tcttccgatt ccttgagatg gtaatcgccg tccttggcga
     3601 gaaggaagcg ggtcagggcc ttctcttcgg cgaagagttt gttggtgagc gaggtcgcgc
     3661 cggtcacgcc cttttcaccc agtcggatat ctatcttctc ggcgaagggt tcggtcgcct
     3721 tcacctgaag gatgccgacg gtgctggact gcaggaggac cttgaggagt tctatcggtt
     3781 ctatcttgga gagttttccc tccttggcgt gaagcgaacc gtcgaagcgg gtctgggcca
     3841 tggccgcctt gaggtcgcgt ttctcgagag agaccttggc gacattgacg ccgcgcggaa
     3901 tgctgcgttc gtcatcgttg gtgagactgt cgagcgccgc atcgagcgcg gcaggcgccg
     3961 cagtcgccgg gtcggccttc cttttcggca ggggtatcgc gtcgggacgg tcgcctatct
     4021 cctgcggcgc cggttgcgcg ggcgtcgccg aagcgaccgg ttttctgatc tccgcctttg
     4081 catcggtccc tttggcgccg gtgaaggtga aggtcgattc gccgatggtg atctcgtcgc
     4141 cgggggagag ccggatcggt tcgcccgcct tcagaggttt accgttcagc aaagaaccgt
     4201 tggtgctgtt cgagtcctcg atatgggtgt ttccgttgtt gctccagata cgggcgtgct
     4261 tgcgggagac ctttgcgtcg atgatggcga catcgcaaga gatgtcgcgc ccgatgatga
     4321 tctgggcgtc gcgcgaaaca gtgaactcgc cgcccttgta ttttccttcg gtgaacacga
     4381 aaaagaagcg attcccttcc tccgcgttct tttggagaag tgagctgaat tttcccatga
     4441 cccgggtctc ctacctcgcc gaatgatggg gaatcataac aaagcaaaaa atgttttcaa
     4501 gaaattcgag cggttattcc cgtttttttc aggagccttg taagaggtgc gcgtctatcg
     4561 ttccccggaa tggtgcccaa cggtcttccg tcgggacggg ggaagagacc cgcatcggtt
     4621 catgtttcac cggatggtcg aacgatatcg atacggcgtg cagggcgagg agactgtgtt
     4681 ccgcgccgct cttgggcccg tatttctcgt caccgatgat cggatggccg acggtggcga
     4741 gttgcacccg tatctgatgc gaccgaccgg tcaggagttc gaccgaaagg agtgaatgat
     4801 cgccgtgcgt cgcaaggacc cggtaggtca gtatcgcccg tttcgaccca ggcgtaccgg
     4861 gggctgagac caagacgagg ttccgtgaag ggtccttgag aagatggtgt tcgagccgtg
     4921 cctcggaggg cgccggtccg ccgcgtacga cggcgaggta ggtcttgtgg aacagaccgg
     4981 cgcgaagttg ttccgcaagg cgcgacgccg ccttcgatgt ctttgcgaac agcacgacgc
     5041 cgccgaccgg ccggtcgagc cggtgcacca tgccgagata ggccgccccc ggctttctgt
     5101 aacgttcttt gaggtgttct ttcaccgtgg tcaacaggtc cgggtcgtcg ctacggtccg
     5161 cctgtgtcgg catgccgcgc ggcttatcca ccgcaatgag atgattgtcc tcgaacagga
     5221 tgggtatgtc catcggtcag tccgtcagat ggacgatcag ttcccgccgg ggaagcgggc
     5281 ggagcgccga tcggtcgccg tcgggggtca actcctcggc ggcgtacccg aaaaagagtt
     5341 cttcgatggt gccgccggtc gtatcggtgt cggccgaggt atcctcgaag agtatggtca
     5401 gggtgttcga tgagtcgttg gtgaagagaa gacccggggc gaccgggatc gcgtcgggct
     5461 cgttgaccga ccacatcgaa cggaggcaac gggtccaggt gccgcggcaa agccattctt
     5521 cgtcggagag ccagcggccg gcgagtatgc cgttgatcca tatcgtcgct ttggcgttcc
     5581 tgcccttgag cacgagtgcc ggtggtgcgt cgaagccgtc acgcagtatc gccgtggtcg
     5641 tgaaggggac gcggtaccag accatgcctc ctttcggtag cgtggcgggg cgctccccct
     5701 tcggccagac gagttcgttg ctgtcggtat catgccagct atcgagttcg ccgtcggtcg
     5761 ctgcgcgcag gcgccacgag gcgaggtcgt aggtcgcggt cgatccgttc accttgccgc
     5821 ggaaataggc ggatccccag agtcctttga gggaatcgaa gccgagcgag ggcatctgtc
     5881 ccggaaaacc ggccatcttg ccgcggggcc acatgaaact gccatgtccc catatctcca
     5941 cgcggaaagc gataaggttc tcgccggcat gaaggagcgg tgcgatgtcg gcgaatcggt
     6001 accgttcgga gccgtcggag tcgatctcgg tgccgaccgg ggaaacggtg gtgacataga
     6061 cgccgttcac atagatgccc acgatatcgg aagcgtgttc aatgtacagc cgtccttcgg
     6121 tcggcgtacc cggaagatcg atcgtggccc ggtaccatgc gtgccccgaa aggatgtcga
     6181 attcctcaag cggttcgggt tcgccggtcc aggtgatcca gtcgctgtca tcgtacccgg
     6241 gcatcgctgc cgcgccgtcc tcgaggagcg cgccgatgcc gagaagggtc gggtccaccg
     6301 ggagtggagc tgaccaggcg ggcaagcgcc agcgcgagag accggtcgtt tcgtcgcggg
     6361 gtgtcccgat agagagttcc ggtatcgtca tcccgacggg agtgatgagc gccagcgatc
     6421 cttggcgttc atcgtactcc atgcgcaggg tgccgtcaga ggccgattcg gcgcggtcgg
     6481 gaccgacgag gataagatcg tgaccgcgtg accggagcgc ccagaacctt cccgccgcgt
     6541 cgctgtcgag gatgagcacg cgcaccgtcg tgccgcccgc cg
//
LOCUS       DAOQUE010000107         6495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_13088_length_6495_cov_3.966770, whole genome shotgun sequence.
ACCESSION   DAOQUE010000107 DAOQUE010000000
VERSION     DAOQUE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13088_length_6495_cov_3.966770"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..885)
                     /locus_tag="PKH10_04585"
     CDS             complement(79..885)
                     /locus_tag="PKH10_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT27440.1"
                     /translation="MSDRLANKIALITGAARGIGEATARLFHREEATVIVSDIRDDMG
                     KGVAAGLGTRAEYLHLDVRSEADWQKAATYLSTKYGGVDIVVNNAGITGFLETAGPHD
                     PEHCDLASWDEVHRVNLTGVMLGCKHAIALMRKRGGGSIVNISSRSGIVGIPGAAAYA
                     SSKAAVRNHTKSVALYCAEKNYAIRCNSIHPAAIMTPLWDAMLGEGAAREKALRGIEA
                     GIPMGKMGDPLDVAYGALYLASDESKYVTGTELHIDGGILAGAEATPRHD"
     gene            complement(962..1108)
                     /locus_tag="PKH10_04590"
     CDS             complement(962..1108)
                     /locus_tag="PKH10_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27441.1"
                     /translation="MAACRYILRTQLFKGRICPALEEASSKKVIDTIKGGSPLYIWTD
                     DRTK"
     gene            complement(1134..1760)
                     /locus_tag="PKH10_04595"
     CDS             complement(1134..1760)
                     /locus_tag="PKH10_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27442.1"
                     /translation="MGKIVKFRCGPISILGMVIVLLCCVQCDSARPGTDSALIYKQAF
                     DTAVEYMRQHNVRAIGEYKELRIYPDTTKKEYREIQCGRASPYEAALCREKVLDSPEH
                     KEKVEKFAQIGRKAALDREAMKSTPYVIAQCVKASAESRSRCEKGLTINHEYFSHIGD
                     EEKVTIVHFIKKPAGEVFLSDIETLLILDAAAKKVVGYYELSGGLSAQ"
     gene            complement(1782..3182)
                     /locus_tag="PKH10_04600"
     CDS             complement(1782..3182)
                     /locus_tag="PKH10_04600"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT27443.1"
                     /translation="MRRAGVAYLFPAPRRVALSNLAPHYFVNAFRDSHGLDLLFEGED
                     RSLIDRKRPNEFAVIFVGLSYEPSYLNFARYLHLHGIPILRSDRPDGKFPLIIAGGVA
                     PSLNPQPLLDICDAVLCGEAEPMHGDLTAILNDPAAAREVLPRLYYAAKPGKKPRIAQ
                     AAAGSFAVSSHPALTGEGNEFGGRTVLELDRGCVGTCHFCAARHLYGASREADRHRIT
                     HAAEEAFKRGKDIALLGTGLESVSYFDDLLDRAIAAGRTVSLSSVRIAGFTPQRASRL
                     AAAGVKTVTLAPESMVVATRKRIGKPLTDDELFAAFETAKAHHFKVKLYLMAGLPDSD
                     SAAEAQAIGAFFAELKDRRLTVPLSLSVTPFCPKPGTPFRGKALMPKKEYERFRDTVQ
                     RGAGKLMPQMPVEWFSWRESLLQTAFDRMSAAQGAAFLAVYADGGELRAAEKRSGIRL
                     EDAANETQKKGTAQSK"
     gene            complement(3179..4609)
                     /gene="ftsZ"
                     /locus_tag="PKH10_04605"
     CDS             complement(3179..4609)
                     /gene="ftsZ"
                     /locus_tag="PKH10_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNT27444.1"
                     /translation="MEQTKSMPLVRIGVVGVGGAGGNAINRIVDERDRLAREGGESVF
                     SEVTLIAANTDAQDLGASGAARRVQLGVNKTKGRGAGSCPEVGRESAEENRVDIANTV
                     DGIELLFITAGMGGGTGTGASPIIAEVARAEGALVVGVVTTPFRFEGKHKMKVAQAGL
                     DRLREQVDALVVIPNEKLLTTDGERLGVIESFRRSDEVLISAVRSVSTIIAKKSLINI
                     DFADLCHTMRGMGTAMIGIGSATGENAAVNAIKEALGNPLMSDVSIKGARRALLHIGG
                     MQIALCELDEAAKYLEGQLHEEANLIWGAAIDETADKIQVLVIAEASPATAPAVVRPA
                     DAALRNVPKNAQVSLLDMANAPAAAPQEAKAPPAPVEPPPLAIPSAAVARQALPEVKG
                     VFREKERPVRLDYSEKILVHLPDETPPDPNNKEIPAYLRPRLTSRQKNGEPPAPRPPA
                     PPRRQDERQTVLQTFTGAANNKNGDE"
     gene            complement(4628..5857)
                     /gene="ftsA"
                     /locus_tag="PKH10_04610"
     CDS             complement(4628..5857)
                     /gene="ftsA"
                     /locus_tag="PKH10_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNT27445.1"
                     /translation="MSKNESIITALDVGTSKVTTLVASRNAAGRIQFIGKGVARNPDG
                     MINGNVVNMEATTRAINESVTEADKICDVRIRSVLLGLSGEHISSLNSHGVFTVRGRE
                     VAQSDIDRVVEQSKNISLSGERRIVGTEMGEFVVDEQSGIKNPRGMAGRRLEVKTHVV
                     TASVAMIQNLVRSVTDAGLQVDEILVNGIASAESVLQEDEKQLGVALVDIGDGTADIV
                     VYKQGALVYTAVVPMGGRYITRDISIALRMPPAEAERVKCRFGCAEPKSVSPDEEIEV
                     SCIGDEEKKIIKAQFVAEVITPRVEEIMVNIRKKINEDIKDSFISSGIVLTGGTANLR
                     YIKNIAEDVLGLPVRIGRPAISDDGMGFAELLRDPSFASAVGMAYYHGRRGGVKNGSS
                     HGGLFGWLGRVMEKFFQ"
     gene            complement(5870..>6495)
                     /locus_tag="PKH10_04615"
     CDS             complement(5870..>6495)
                     /locus_tag="PKH10_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNT27446.1"
                     /translation="DHRVRWFDLDEHRIGLFIRSNGWIKECSVAARFPDRVHISVTEY
                     QPAIVVNRPEASGERGERLYSIWFADAEGAVFKKAFPCEGREALPLLFIEDEVDAGHT
                     RDVIRQALSVAEEWRPRADICRLRSIAYDVFDGFSLECEFPGRRIARVMIGDLAGDDT
                     LLRRRGRIFFESTEKLHERSLFAAEYLFDPHREDDALVAGRIVHYVP"
BASE COUNT         1155 a         2083 c         1925 g         1332 t
ORIGIN      
        1 tttagtgccc tccaccccgg tgttttgtca ccatcatata tgcgggaaca gcgccaccga
       61 agaaaaatag caaattcttc aatcatgtct gggcgtcgct tccgctccgg caagtatacc
      121 tccgtcgata tgcaactcgg tccccgtcac atacttcgat tcgtcgctcg ccagatacag
      181 agcgccgtac gccacatcga gcgggtcccc catctttccc atcgggatac ccgcctctat
      241 gccgcggagc gctttttcgc gcgccgcgcc ttcccccaac atcgcgtccc acaagggcgt
      301 catgatcgcc gccggatgga tggagttaca gcggatcgcg tagttctttt ccgcgcaata
      361 gagcgcgaca ctcttggtat gattccgtac cgccgccttg ctcgatgcgt aagccgccgc
      421 gccgggaata ccgacgatac cgctgcgcga cgagatgttc acgatactgc cgccgccccg
      481 cttccgcatg agcgcgatgg catgtttgca ccctagcatc acgccggtca gattgacccg
      541 atgcacctca tcccacgacg cgagatcgca gtgttccgga tcgtgcggac cggccgtttc
      601 gagaaaaccg gtgatcccgg cgttgttgac gacgatatcg acaccgccat atttcgtgct
      661 cagataggtg gccgcttttt gccaatcggc ctcggaacgg acatcgagat ggagatattc
      721 ggcccgcgta ccgagcccgg cggcaacgcc cttgcccata tcgtcgcgaa tatcgctcac
      781 gatcaccgtc gcctcttcgc ggtggaacaa acgggcggtc gcttcgccga taccacgcgc
      841 cgccccggtg atgagcgcta ttttattcgc cagtcgatcc gacatgcgat tcctccgttt
      901 gaaaaataac ggcgcagtgt atcaccggag aaaaacggag tcaactggaa agaaatactc
      961 actacttcgt gcggtcgtcg gtccagatgt aaagaggcga cccgcccttg atggtgtcga
     1021 tgaccttctt tgagctcgcc tcctcgagcg ccgggcagat gcgtccctta aagagttgtg
     1081 tccgtaagat ataccggcaa gccgccaagc gctgcaaacg atagagacga cgattactgc
     1141 gctgatagtc caccggagag ctcataatag cctacgactt ttttcgccgc ggcatcaaga
     1201 atcaagagcg tttctatgtc ggaaagaaat acctcgccgg caggcttttt tataaaatga
     1261 actatcgtta ccttctcttc gtctccaata tgagaaaaat actcatgatt aattgtaagt
     1321 cctttttcac atcgtgatct actctcggct gatgctttta cacattgagc aataacatac
     1381 ggtgtactct tcatcgcttc tctatcaagt gcggccttcc taccgatctg cgcgaatttc
     1441 tctaccttct ctttgtgctc tggcgagtca agaactttct ctcgacaaag agccgcttcg
     1501 taaggcgatg ccctaccaca ctgtatttcc cgatattcct tttttgttgt gtcaggataa
     1561 atacgcagtt ccttgtattc tccgatagct ctcacattat gttgtcgcat atattcaact
     1621 gcggtatcaa atgcctgttt ataaatcaac gccgaatccg tgccgggacg ggccgagtca
     1681 cattgtacac aacacaacag gacgataacc atcccaagaa tagaaatagg gccacatctg
     1741 aattttacta ttttacccat cgacacctcc cccggcttcc tctatttgct ttgggcggtt
     1801 cctttttttt gcgtctcatt cgctgcatct tccagccgta tccccgaccg tttctcggcg
     1861 gcgcgaagtt cgccgccatc ggcgtaaacg gcgagaaagg cggccccttg ggcggcgctc
     1921 atccggtcaa aagcggtctg caggagcgat tcgcgccacg agaaccactc gaccggcatc
     1981 tgcggcatca gtttccccgc accgcgctgg acggtgtcgc ggaagcgttc atattctttc
     2041 ttcggcatga gcgccttacc acggaacggc gtgcccggct tgggacaaaa gggggtcacc
     2101 gaaagcgaga gcggcaccgt cagtctgcga tctttcagtt cggcgaaaaa agcccctatc
     2161 gcctgcgcct cggcggcgct gtcactgtcg ggaagcccgg ccatgaggta gagtttcact
     2221 ttgaaatggt gcgccttggc ggtctcgaag gcggcgaaca gttcatcgtc ggtaagcggt
     2281 ttgccgatgc gcttgcgggt cgcgacgacc atgctctcgg gagcgagcgt caccgtcttc
     2341 accccagccg ccgcgagccg tgatgcccgt tgcggcgtga agccggcgat gcggaccgac
     2401 gagagcgaca cggtgcgccc cgccgcgatg gcgcggtcga gcaggtcgtc gaagtaggat
     2461 acgctctcaa gaccggtccc gagaagcgcg atatccttgc cccgtttgaa cgcctcctcc
     2521 gccgcgtgag tgatgcggtg gcggtcggcc tcacggctcg ccccgtaaag gtggcgcgcc
     2581 gcgcagaaat ggcaggtccc gacacagccg cggtcgagtt cgagtaccgt gcgaccgccg
     2641 aactcgttcc cctcgcccgt caacgcggga tggctgctca cggcgaaaga acctgccgcc
     2701 gcctgcgcga tccgaggctt tttccccggt ttcgccgcat agtaaagtcg cgggagaact
     2761 tcacgcgccg cagcggggtc gttaagtatc gccgtgagat caccgtgcat cggctccgct
     2821 tcgccgcaca ggaccgcgtc gcagatgtcg aggagcggtt gcggattgag cgacggcgca
     2881 acgccccccg cgataatgag cgggaacttc ccgtcgggac ggtcgctcct gagtatcggt
     2941 atgccgtgaa gatgcaggta cctcgcgaag ttcaggtagg agggttcgta ggagagcccg
     3001 acgaagatga ccgcgaactc gttcggtcgt ttccggtcga tgagcgaccg gtcctccccc
     3061 tcgaacagca ggtcgagacc gtggctgtcg cggaaggcgt tcacgaaata gtgcggggcg
     3121 agattggaga gcgcgacgcg gcgcggcgca gggaagagat aggcgacccc cgcgcgtctc
     3181 actcgtcgcc gttcttgttg tttgcggcgc cggtgaaggt ctgcaggacc gtctgccgct
     3241 catcctgtct ccgcggcggc gcgggcggcc gcggcgcggg cggctcgccg tttttctggc
     3301 ggctcgtcag gcgcggtcgc agataggccg ggatctcctt gttgttcggg tcgggcggcg
     3361 tctcgtccgg cagatggacc agtatctttt cggagtaatc gaggcgcacc ggccgttcct
     3421 tctcgcggaa cacccctttc acttcgggca gggcctgacg ggcgaccgcc gctgaaggta
     3481 tcgcgagcgg cggcggctcc acgggggccg ggggcgcctt cgcttcctgc ggtgcggccg
     3541 ccggggcatt cgccatgtcg aggagcgaga cctgcgcatt tttcggaaca ttgcgcaacg
     3601 ccgcatcggc ggggcgcacg acggcgggcg ccgtggccgg cgaggcctcg gcgatcacga
     3661 gcacctgtat cttgtcggcc gtttcgtcta tcgccgcgcc ccagatgagg ttggcttcct
     3721 cgtgcaactg accttcgagg tacttggcgg cctcgtcgag ttcgcagagc gctatctgca
     3781 tgccgccgat gtgcaggaga gcgcgacgcg ccccctttat cgagacatcg gacatgagcg
     3841 ggttgccgag cgcctccttt atcgcgttga ccgcggcgtt ctcgccggtg gccgagccga
     3901 tgccgatcat ggcggtcccc atgccgcgca tcgtgtggca gaggtcggcg aaatctatgt
     3961 tgatgagcga tttctttgca atgatggtgg ataccgaccg caccgcgctg atgagcacct
     4021 catcggaacg gcggaaactc tctatgacgc cgagccgctc gccgtccgtg gtgagcagtt
     4081 tctcgttcgg gatgacgaca agcgcgtcga cctgttcgcg tagccggtcg agccccgcct
     4141 gcgcgacctt catcttgtgt tttccttcga accggaacgg ggtggtgacg accccgacga
     4201 cgagcgctcc ctccgcgcgg gccacctcgg caatgatggg cgacgctccg gtcccggtgc
     4261 cgcctcccat accggcggtg atgaacagca gttcgatccc gtcaacggtg ttcgcgatgt
     4321 cgacgcggtt ctcctcggcc gactcgcggc cgacctcggg acacgaaccg gcgccccgcc
     4381 ccttggtctt gttgacaccg agttgcacgc gccgcgccgc gcccgacgcg ccgagatcct
     4441 gagcgtcggt gttcgccgcg atgagcgtca cctcgctgaa aacgctttcg ccaccctcgc
     4501 gggcgagacg gtcgcgctcg tcaacgatgc gattgatggc gttgccgccg gcgccgccga
     4561 cgccgacgac gcctatcctg acgagcggca tgcttttcgt ctgttccatg accgtcctcc
     4621 cgttacgcta ttgaaagaat ttttccatga ccctgccaag ccagccgaac agaccgccat
     4681 gcgacgagcc gttcttgacg ccgccacggc gcccgtggta gtaggccatg ccgacagccg
     4741 acgcgaacga cggatcgcgc aacagttcgg cgaagcccat gccgtcgtcg ctgatggcgg
     4801 gtcgcccgat gcggaccggc aggccgagca catcctcggc gatgttcttg atgtaccgca
     4861 gattcgccgt accgccggtc agcacgatgc ccgacgagat gaaactgtcc ttgatgtcct
     4921 cgtttatctt cttgcggatg ttgaccatga tctcctcgac gcgcggcgtg atgacctcgg
     4981 cgacgaactg cgccttgatg atcttcttct cctcgtcgcc gatgcaggag acctcgatct
     5041 cctcgtcggg actgacagat ttcggctcgg cgcacccgaa ccggcacttg acccgttcgg
     5101 cctcggcggg cggcatacgc agggcgatgg agatgtcgcg ggtgatatag cggccgccca
     5161 tcggcaccac ggccgtatag acgagcgccc cctgtttgta cacgacgatg tcggcggtgc
     5221 cgtcaccgat gtccacgagc gcgacgccga gctgtttctc gtcctcctgc aggaccgatt
     5281 cggccgacgc gataccgttc acgaggatct cgtcgacctg cagaccggca tcggtcacgc
     5341 tccgcacgag gttctgtatc atcgcgaccg aggcggtgac cacatgggtc ttgacctcca
     5401 gccgcctgcc cgccatgccg cgcgggttct tgatgccact ctgttcgtcc accacgaatt
     5461 cgcccatctc ggtgccgacg atgcgccgtt cgcccgacag gctgatgttc ttcgactgct
     5521 ccaccacgcg atcgatgtcc gattgcgcca cctcgcgtcc ccgcacggtg aacacgccgt
     5581 ggctgttcag cgaactgatg tgctctcctg acaggccgag cagtaccgag cggatgcgca
     5641 catcgcagat cttgtcggcc tcggtgaccg actcgttgat ggcgcgggtg gtcgcctcca
     5701 tgttgacgac attgccgttg atcatgccgt cagggttgcg cgccacccct ttcccgatga
     5761 actggatcct gccggcggcg ttccggctcg ccacgagcgt cgtgaccttg ctcgttccga
     5821 catccagcgc ggtgatgatg ctttcgttct tcgacatcgc tatccctcgt tacggtacat
     5881 aatgaacgat cctgccggcg acgagcgcgt cgtcctcccg gtgcgggtca aacagatatt
     5941 cggcggcgaa caggctccgc tcgtgcagtt tctcggtcga ctcgaagaat atccggccgc
     6001 gacgacgcaa cagggtgtcg tctcccgcga gatcgccgat catgacgcgg gcgatgcgac
     6061 ggccggggaa ctcgcactcg agcgagaagc cgtcgaacac atcgtacgcg atgctccgca
     6121 ggcggcagat atcggcccgg ggtcgccact cttcagcgac cgaaagcgcc tgccggatga
     6181 cgtcgcgggt gtggccggcg tccacttcgt cctcgatgaa caggagcggt agcgcctcgc
     6241 gaccttcgca cgggaacgcc tttttgaaca ccgccccttc ggcgtcggcg aaccagatgc
     6301 tgtagagccg ctcgccgcgc tcgccgctcg cctcaggccg gttgaccacg atggcgggct
     6361 gatattcggt gaccgagata tggacgcggt cggggaaacg cgcggcgacc gagcattcct
     6421 ttatccaacc gttcgaacgg atgaagagcc ctatccggtg ctcgtcaaga tcgaaccacc
     6481 ggacgcgatg atcga
//
LOCUS       DAOQUE010000108         6461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_13199_length_6461_cov_4.427724, whole genome shotgun sequence.
ACCESSION   DAOQUE010000108 DAOQUE010000000
VERSION     DAOQUE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13199_length_6461_cov_4.427724"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1481)
                     /gene="murF"
                     /locus_tag="PKH10_04620"
     CDS             complement(144..1481)
                     /gene="murF"
                     /locus_tag="PKH10_04620"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNT27447.1"
                     /translation="MLTHTMINEALARCGSSGRAAIGNFCAVTLDSRTARPGDIFCAF
                     KGERADGHAFVATLCAQGVLCIGSEPMTVTGYARVADVTRFLAVFARLKRDTLPAQVL
                     AITGSSGKTTTRQFAARMLALSGKSVHGTSGNLNNHLGLPLTILNAPEDREIIVLEMG
                     MNHAGEIAHLCAVADPDAVLITNIGFAHIGNFASRDGLAAAKLEIFDHSRGRVVADID
                     DPYIARWLAAHPERSATTYSAAGSAADLTVCDAGDGRMVLRWEGVEYAAPLPDLPDYM
                     VQNFAAAAALVLPYVASPAPLFGVFEDLALPPYRGERVVRGEQVFIADCYNANPDSML
                     RSIENFIIKADGGDHGGRYLVLGDMAELGNFSENFHRELVKKIKTLTVIDKTFLLGEE
                     FEKIADDFIEDERFLLFHSNKELAAALPPRGLFLVKGSRRNRLETIFEPVGGR"
     gene            complement(1481..2932)
                     /locus_tag="PKH10_04625"
     CDS             complement(1481..2932)
                     /locus_tag="PKH10_04625"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNT27448.1"
                     /translation="MRLLDIKRILAADIIADAATANGQEVSDIVVDSRRAAPGTVFFA
                     VPGTRFDGHDFVGQALGNGAAAAFGVRPMTVPGYVQVADIWTAVRAVAPVLYGDPQEH
                     LSLVGVTGTNGKTTVTYLLERMLSSVAPTGRIGTLGYRWGGAEVPAENTTPFPWTWYR
                     TLAAMRRDGVRFVASEVSSHALDQERIAGTRFDAAIFTNFTRDHLDYHASEEAYFAAK
                     RRLFDDHLRDGGLAVLNLDDPKGRELRARIGGARPVVTFAIADQSADLWVRRMTVRGA
                     GYDIVLSYRGADIAARLDLPGRFNVSNLLGALLAASPWMDVARAAALAKGVTVPGRME
                     RVGRRGRIFVDYAHSPDALEKVLAAAREFCGAGRLIVVFGAGGDRDRGKRPLMGAVAA
                     RYADTLIVTSDNPRTEKPEAIIDDITGGIGREQGVEVIVDRSAAIGRAVEVMRDEDVV
                     IVAGKGAEDYQIVGTEKRHFSDHEEIRRHLGEP"
     gene            complement(2932..5010)
                     /locus_tag="PKH10_04630"
     CDS             complement(2932..5010)
                     /locus_tag="PKH10_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNT27449.1"
                     /translation="MKREKRIFGAFAALVGIFFLVVLYRTYHIQIQRHEELDRYSSSS
                     EVVKKIKARRGAIYDHNGAVLAISEPRVDLAVDPNGIKMKKEMAAFLAHEAGLNESEV
                     FRTLGKKSNFRYLRKNVPYETVRAVYEERTATAARLRELERRRGRDDADESERKRLAR
                     WGADLNYVILVDGFNRIYPQGKMLSNVLGWVSKESGEGLGGIESSFNRYLSGGELQVR
                     RLYIPGSGEGALEIDKELESGQAGDIYLTIDSAIQAIAEEELDRMLEKKPALWGGAVV
                     MDPATGRILAMANSPGFDPEEYQKVPLEKRRNYAISSLFEPGSTMKAFTILAAMNEDL
                     VRDGEMFNGHNGVFKFGGRFINDHDKRGIMTLEDVVVHSSNIGTVLIVDRLPSEKMYD
                     YLVRFGFNERSGVQLPGESFKAIRPYQRWFPIGKANIAFGQGLLVNMVQMTRAMSALV
                     NGGVFWQPTIVDRIVDPATGRPLLDMVPSFTRMDLRHNVDKKMLAMLRAVVTKGTGGA
                     SQLKGIPVGGKTGTSQKYDPVKKAYSKEASVCSFLGFAPADNPKLVIMVVIDEPKGRE
                     FGGTVASPVFREIARRALPLFGVYAEKDAKDIAPFPRMAAEEAIVPITGGAEGTEEVS
                     ALEVVTIPMTEGMPVARAVYTLNQAGLDVVVSGSASGTVKRQFPKGGDSVLYGTSVVI
                     ESDESAEEEE"
     gene            complement(5020..5373)
                     /locus_tag="PKH10_04635"
     CDS             complement(5020..5373)
                     /locus_tag="PKH10_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27450.1"
                     /translation="MADLAPVRSSKKETILPVILEVRKVQAKLDLFSPPLLLFMAGTV
                     VLALFLTFDSYQKSYRNAQMVRISGAADTLRVDNFRKTELESRYLRLMSAPAVSEQAR
                     DLNMQQATKDRVLNF"
     gene            complement(5395..6306)
                     /gene="rsmH"
                     /locus_tag="PKH10_04640"
     CDS             complement(5395..6306)
                     /gene="rsmH"
                     /locus_tag="PKH10_04640"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNT27451.1"
                     /translation="MSISYRGHRPVLLADFSRMAPDQVSVYVDLTAGGGGHAEQIARR
                     YPAARLILIDRDKEAVERLREKFAGMERVTVVHGRAGEVDKWLFLLKLRKVDLIFADL
                     GVSSHQLDDPARGFNFKSEAPLDLRMDATGGETALDLIKRLTERELQDILSSYGEERE
                     SRTVARALKKAVTEGKRTVKEFRDAIAAVKRFRPARIDPATQTLMALRIAVNDELGEV
                     ERMLERAFVSLAPDGVLGVISFHSLEDRLVKRFFVDRRGQVPLPRPFDDLPPRPVTVL
                     KAVRPTEEEVLVNPRSRSAKMRVLVRN"
BASE COUNT         1106 a         2170 c         1965 g         1220 t
ORIGIN      
        1 ggtaggcgat gaaacgcggg tacagcagga gcgacaggcc gaacccggtg aagaccgcca
       61 tgaaggtgcg gaaggtgatg taactgacca catggaactg cgggaacgag gttatgagca
      121 ggtccttgag atagatgagc atgctaccgg cctcctacgg gttcgaatat cgtctccaga
      181 cggttgcgcc gcgatccttt gacgaggaac agcccgcgcg gcggcaaagc ggcggcgagt
      241 tccttgttag agtggaataa aagaaatctt tcatcttcga taaaatcatc ggcgatcttt
      301 tcgaactcct cgcccagcaa aaaagtctta tcaatgactg taagggtttt tatctttttt
      361 acaagttctc ggtgaaaatt ctctgaaaaa ttacctagtt cagccatgtc gccgaggaca
      421 agatatcggc cgccatgatc gccgccgtca gccttgatga tgaaattctc tattgatctc
      481 aacatgctat ctggatttgc gttgtagcag tctgcgataa acacctgctc gccgcgcacg
      541 acccgttcgc cgcggtatgg cggcagagcg agatcctcga agaccccgaa gagcggcgcg
      601 ggcgacgcga catacggcag gacgagcgcg gcggcggcgg cgaagttctg caccatgtag
      661 tcgggcaggt cggggagtgg cgccgcgtac tccacgcctt cccagcgcag gaccatgcgc
      721 ccgtcgccgg cgtcacacac cgtgagatcc gccgcgctcc ccgcggcgct gtaggtcgtc
      781 gcggaccgtt cgggatgcgc cgcgagccag cgcgcgatgt agggatcgtc gatatcggcg
      841 acgacccggc cccgcgagtg gtcgaatatc tcgagtttcg ccgcggcgag tccgtcgcgc
      901 gacgcgaagt tgccgatgtg ggcgaacccg atgttggtga taagcaccgc atcgggatcg
      961 gccacggcgc acaggtgcgc tatctcgccg gcatggttca tccccatctc gagcacgatg
     1021 atctcgcggt cttcgggcgc gttcaggatc gtcaggggca ggccgaggtg attgttgaga
     1081 ttgccgctcg tcccgtggac gctcttaccc gaaagggcga gcatccgcgc cgcgaactgg
     1141 cgcgtcgtcg tcttgcccga cgaaccggtg atcgcgagca cctgcgccgg gagcgtgtcg
     1201 cgcttgagcc gcgcgaatac cgcgaggaac cgcgtcacat cggcgacccg cgcatacccc
     1261 gtgaccgtca tcggctcact gccgatgcag agcacgccct gcgcgcagag cgtcgcgacg
     1321 aacgcgtgac cgtcggcccg ctctcccttg aacgcgcaga agatatcacc ggggcgcgcc
     1381 gtgcggctgt ccagcgtgac cgcacagaag ttcccgatcg cggcgcgtcc ggacgaaccg
     1441 caccgcgcga gcgcctcgtt gatcatggta tgggtcagca tcagggttcc cccaagtgtc
     1501 ttcgtatctc ctcatggtcc gagaaatgcc gcttctcggt cccgacgatc tgatagtcct
     1561 cggccccctt ccccgccacg atgacgacat cctcgtcgcg catgacctcg acggcccgtc
     1621 cgatggcggc actccggtcg acgatcacct cgacgccctg ttcccgtccg atgccgccgg
     1681 ttatgtcatc gatgatcgcc tctggctttt cggtgcgcgg gttgtcggag gtgacgatga
     1741 gcgtgtcggc ataccgcgcc gcgaccgctc ccatgagcgg ccgttttccc ctgtcgcgat
     1801 ccccgcccgc gccgaagacg acgatgagcc gccccgcgcc gcagaactcc ctagccgccg
     1861 cgagcacctt ttcgagcgcg tcgggggaat gggcataatc gacgaatatc cggccgcggc
     1921 gaccgacccg ctccatgcgg cccggcaccg tgaccccctt cgcgagagcg gcggcccgcg
     1981 cgacatccat ccacggcgac gccgcgagca gcgcgccgag caggttggat acattgaagc
     2041 ggcccggcag gtcgagtcgc gccgcgatat cggcgccgcg gtacgaaagg acaatatcat
     2101 aaccggcgcc gcgcaccgtc atccgcctca cccacagatc tgcgctctgg tcggctatgg
     2161 cgaaggtgac gaccggtcgc gccccgccga tccgcgcccg cagttcgcgc cccttcggat
     2221 cgtcgaggtt caggaccgcg agcccgccgt cgcgcagatg gtcatcgaac agccgccgtt
     2281 tcgcggcgaa ataggcctct tcgctcgcat ggtaatcgag atggtcgcgc gtgaagttgg
     2341 tgaatatcgc cgcgtcgaac cgcgttccgg cgatgcgctc ctgatcgagc gcgtgcgacg
     2401 acacctcgct cgccacgaaa cggacgccgt cgcgacgcat cgccgcgagc gtgcggtacc
     2461 aggtccacgg gaacggcgtc gtgttctcgg cgggcacctc ggcgccgccc cagcggtaac
     2521 ccagcgtgcc gatgcgaccg gtcggcgcga ccgacgacag catccgctcc agcagatagg
     2581 tgacggtggt cttgccgttc gtcccggtga caccgacgag cgaaaggtgt tcctgcggat
     2641 cgccgtagag taccggcgcg acggcgcgga ccgcggtcca gatatcggcc acctgcacgt
     2701 atcccggcac ggtcatcggg cggacgccga acgccgcggc cgctccattg ccgagcgcct
     2761 gtccgacgaa atcgtgtccg tcgaaccgcg tacccggcac cgcgaagaac accgttcccg
     2821 gtgccgcgcg gcggctgtcc acgacgatat ccgacacctc ttgcccgttc gcggtcgctg
     2881 catcggcgat gatgtcggcg gcgaggatcc gcttgatatc caacagccgc atcattcctc
     2941 ctcttccgct gattcgtcgc tttctatgac gaccgaggtg ccgtacagca cgctgtcgcc
     3001 ccctttcggg aactgccgtt tcaccgtccc tgacgccgat ccggatacca ccacatcgag
     3061 tcccgcctga ttgagcgtat agacggcgcg cgcgaccggc atcccttcgg tcatcgggat
     3121 agtgaccacc tcaagcgcag acacctcctc ggtgccttcg gcgcctcccg ttatggggac
     3181 aatcgcctct tcggcggcca tgcgggggaa cggtgctata tctttcgcgt ccttttcagc
     3241 gtacacgccg aacagcggca aggcgcggcg tgcgatctcg cggaacaccg gcgatgcgac
     3301 ggtcccgccg aactcacggc ccttcggctc gtcgatgacc accatgatga ccagtttggg
     3361 attgtcggcc ggcgcgaacc cgaggaagga acagaccgac gcctccttgc tgtacgcctt
     3421 cttgaccgga tcgtatttct ggctggtgcc cgtcttgccc ccgaccggga tgcctttcaa
     3481 ttgcgacgcg ccgccggtcc ccttcgtgac gaccgcgcga agcatcgcga gcatcttctt
     3541 gtcgacatta tgtcgcagat ccatccgcgt gaacgagggg accatatcga gcagcgggcg
     3601 gccggtcgcc ggatcgacga tgcggtccac gatcgtcggt tgccagaaca cgccgccgtt
     3661 cacgagcgcc gacatcgccc gcgtcatctg caccatgttg acgagcagcc cctgtccgaa
     3721 cgcgatgttc gccttgccga tggggaacca gcgctggtag ggacggatcg ctttgaacga
     3781 ctcgcccggc agttgcaccc ccgaccgctc gttgaagccg aaccgcacga gatagtcgta
     3841 catcttctcg gaagggagcc ggtcgacgat gagcactgtg ccgatgttcg acgagtgaac
     3901 gaccacatcc tcgagcgtca tgatgccgcg cttgtcgtgg tcgttgatga accgtccgcc
     3961 gaacttgaac acgccgttgt gaccgttgaa catctcaccg tcgcgcacga ggtcctcgtt
     4021 catcgcggcg agtatcgtga aggccttcat cgtggagccg ggttcgaaca aactggatat
     4081 ggcgtagttg cgccgctttt cgagcgggac cttctggtat tcctcggggt cgaagcccgg
     4141 gctgttcgcc atcgcgagta tgcgaccggt cgccgggtcc atgacgaccg caccgcccca
     4201 gagcgccggt ttcttctcga gcatccggtc gagttcctct tcggcgatcg cctgtatcgc
     4261 gctgtctatc gtcaggtaga tatcgcccgc ctgcccgctt tccagttcct tgtctatctc
     4321 gagcgccccc tcgccgctcc ccggtatgta gagccggcgc acctgcagtt cgccgcccga
     4381 caggtagcga ttgaacgaac tctcgatgcc gcccagcccc tcgccgctct ccttggagac
     4441 ccagccgagc acattcgaca gcatcttccc ctgcgggtag atgcggttga acccgtcgac
     4501 gagtatcaca tagttgagat cggcgcccca gcgcgcgagc cgtttgcgtt ccgattcgtc
     4561 ggcgtcgtcg cgtccgcgcc gccgctccag ttcccgcagg cgggcggcgg tcgccgtccg
     4621 ctcctcgtac accgcgcgga ccgtttcgta cgggacattc ttccggaggt accggaagtt
     4681 gctcttcttc ccgagcgtgc ggaacacctc ggactcgttg agccccgcct cgtgcgcgag
     4741 gaacgccgcc atctcctttt tcatttttat cccgttcgga tcgacggcga gatcgacgcg
     4801 cggctccgat atcgcgagca ccgcgccgtt gtggtcgtag atggcgccgc gccgcgcctt
     4861 tatcttcttg accacctcgg aggaggagga gtagcggtcg agttcttcgt gccgttgtat
     4921 ctggatatga taggtcctat agagtacgac gagaaaaaag ataccgacca gcgctgcgaa
     4981 ggcgccgaat atccgttttt cccgtttcat cagcccacat cagaaattca gtacgcgatc
     5041 cttcgttgcc tgctgcatgt tcaggtccct cgcctgctcc gacaccgcag gggccgacat
     5101 gagccgcagg tagcgcgact ccagttcggt cttgcggaag ttgtcgaccc gcaacgtgtc
     5161 ggccgccccg ctgatgcgga ccatctgcgc gttgcggtaa ctcttctggt aggagtcgaa
     5221 ggtcaggaac agcgcgagta ccaccgtccc ggccatgaac agcaggagcg gcggcgagaa
     5281 caggtcgagt ttcgcctgca ccttgcggac ttcgagaatg accggcagta tcgtctcctt
     5341 cttcgacgat cgcaccgggg cgagatcggc catgggtccc ctccttcgta tcgttcagtt
     5401 ccttaccagc acccgcatct tcgcgctccg cgaccgggga ttgacgagca cctcctcctc
     5461 ggtgggccgg accgccttca gcacggtcac cgggcgcggc ggcagatcgt cgaagggtct
     5521 cgggagcggc acctgacccc gacggtcgac gaagaaccgt ttcaccagac ggtcctcgag
     5581 cgaatggaac gatatgacgc cgagcacccc gtcgggggcg agcgacacga aggcccgttc
     5641 gagcatccgt tcgacctccc cgagttcgtc attcaccgct atgcgcaggg ccatcagcgt
     5701 ctgggtcgcc gggtcgatgc gcgccgggcg gaaccgtttg accgccgcga tcgcgtcgcg
     5761 gaactccttc acggtgcgct tgccttcggt caccgccttc ttgagcgccc gcgcgaccgt
     5821 ccgcgattcg cgctcctcgc cgtacgacga gagtatatcc tgcagttccc tttcggtgag
     5881 ccgtttgatg agatcaagcg ccgtctcccc gccggtcgcg tccatgcgca ggtcgagcgg
     5941 cgcctcgctt ttgaagttga atccgcgcgc cgggtcgtcg agttgatgac tgctcacgcc
     6001 gaggtcggcg aagataagat cgaccttgcg caacttgagg aggaacagcc atttgtccac
     6061 ctcgccggcg cggccgtgca ccacggtgac ccgctccatg ccggcgaact tctctctcag
     6121 ccgctccacc gcctctttgt cacgatcaat gaggatgagc cgcgccgccg gatagcgccg
     6181 cgcgatctgt tcggcgtgtc cgccgccccc cgccgtcagg tcgacatata ccgagacctg
     6241 atctggcgcc atccgcgaga aatccgcgag aagcaccgga cggtggccgc ggtatgagat
     6301 cgacatgagg catcacccct tctgttctag gttcttcgcc cggttgagta tatcgtatat
     6361 ctgttcgaac gacgccggcg ccgtcttctc gcgctccgcg agcgactctt tcgcccacac
     6421 ctcgatgcgg tcgatgcagc cgagaagcac cgtctccttg t
//
LOCUS       DAOQUE010000109         6442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_13245_length_6442_cov_3.702208, whole genome shotgun sequence.
ACCESSION   DAOQUE010000109 DAOQUE010000000
VERSION     DAOQUE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13245_length_6442_cov_3.702208"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2259)
                     /locus_tag="PKH10_04645"
     CDS             complement(<1..2259)
                     /locus_tag="PKH10_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT27452.1"
                     /translation="MRALFPSFIQERFLAGELHGAFRGGAMFVDVSGFTSMTEKLMQH
                     GKVGAEFISDILNSTFSPVIDAVYQSGGFITGFAGDAFTAVFPGEDILRPCHAGREIQ
                     RIFGIRSCHETPYGTFTISAQVGISFGDVHWGIPGTDYHRTFFFRGEAIDGCAAAEHH
                     ACSGDVLLDDRALAKSPEKAFSTGPTHDDAAGFHRIASWTDAVSAPVSRPEDPIRPEV
                     GRVFYPEQIYSQKLSGEFRDIVPVFISFQQPDGFEALDRFAAAVIETCDAFGGYFNLL
                     DFGDKGGNMLVLFGAPVSYENNVQRAVSFILAVKERGELPFRCGITQGEVYAGFVGSD
                     RRSTYTVLGDRVNLSARFMMKAAWGDIWLSRMVADKIADQYETGLIGEFQFKGKTEKT
                     PVFSLRGPKAAGDELAFEQAMIGRDAELDRATTALGPLAEGRNGGLLMIWGDPGIGKT
                     RFLAAVKDRLAGTAEVIVLSADSILRKSMNPFVNFFERFFDQGDADSPEEKKRRFEKR
                     WDAIIAKIPPSAQEVVAELSRTRSFLGALIGIEWKDSLYEKLDAKGRFENTLYAVKEF
                     LKALSLIHPVAIALDDVQWLDNDSREIVKILFRQTDRFPFALITLGRYEDDGTKTNIE
                     TDPGIARTEIPLDRLSPDSVALLAKTVLGAPIGDNLRSLIMQRSEGNPFFVEQICFYL
                     KNNNLLELRDDHFDAIGGDVEIPQGVNALLISRIDRLSQRLKEGVFTASVLGREVDVN
                     IFAALMSEHDVPT"
     gene            2375..2605
                     /gene="nrdD"
                     /locus_tag="PKH10_04650"
     CDS             2375..2605
                     /gene="nrdD"
                     /locus_tag="PKH10_04650"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNT27453.1"
                     /translation="MARHEPEEYAVEGTKQPDNSKKCELSCEVWSRVVGYYRPTGNWN
                     RGKQEEFKKRKPFVVNAVAFPGGPEPHIETPR"
     gene            2625..3464
                     /locus_tag="PKH10_04655"
     CDS             2625..3464
                     /locus_tag="PKH10_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27454.1"
                     /translation="MSLLKRSATVLVGRILLFIAIATFFWIPMIRHYAIRYRLLGGSW
                     WEHIHPAQFDWLLIGIFFFMTAICVFTIDLRKNLLILLIAALGGLSIEGWGTQTELWR
                     YYTRERPPLWIIPAWPTAALTIDSLSRLVDRRIPHIDAKWWNGAAWVFFGSFLALMIP
                     FTLPYLSYSMTIFSIAVIALVIVSLWAGTADRRTAVVIFVVGSALGWFLELWGTTRHC
                     WIYYTGQEPPLFAVLAHGAASVTFWRAHLIADGIITRLFPNYRGHWSRGAVAERNGSP
                     ISR"
     gene            complement(3382..4254)
                     /locus_tag="PKH10_04660"
     CDS             complement(3382..4254)
                     /locus_tag="PKH10_04660"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNT27455.1"
                     /translation="MLDWLTLRDPYIGGYGYNVFMWGGFAVWIWLTVRELSFLKEFWN
                     LFRIVFFSFVGAVLIPLSILLIQTALNGQLFNYTSGVSQLIFGIYTPEGFVKFFKFAF
                     KGFSITAGILTLSAILLFIRKDWRRFFYPFVYPFPLFSAIIRLGCLADGCCFGRPTDL
                     PIGMVFPPGSYASALHYKYGQIASRYAPSLPVHPVQVYISASMLLLFVAIVVMERRGI
                     RRDIIAGVCLVGYGVANFLIEFVRQESRIVFSLLTAGQVIEILVALIGIWVIRSALRP
                     PPGSSAPDSSGTTA"
     gene            complement(4264..5814)
                     /locus_tag="PKH10_04665"
     CDS             complement(4264..5814)
                     /locus_tag="PKH10_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNT27456.1"
                     /translation="MMTRVAKIGIFFIVITALTSYYMMKSADWLMKGGTYRLHAYLDD
                     ATGLLEDSEILIAGVAVGKIRTIELEGNRAKVTLEIYDKVALYKDAFLYKDMQSMLGT
                     AMLQLSAGEDTANPLKDGDWITHVDSQTGLNYTIGKAKSIANRAEGIVNNIGDFVERE
                     DFQKKMTELIDVLTKVSRSSAQNVEANLVLMRITLKNMADITGKVNANFDRNLAQMEL
                     LIKNTIAITDKANKLIGDNDQTISDSLAAVRDSLQALSGQIKDAQETLESAKATMKNM
                     EDITGRVKDGEGNLGKALKDEKLYDHLTNIAEKASDYADSTLGMKVHVDFHSEFRTLD
                     QNFQNQFALKLQPRDDRYYLIGITDDPGGRTTEKTTVTEITDPAETPTVRTVTEHETT
                     TEKSGIKFDLQIARIFGDWTLRSGIIESSGGVGVDYLPVKYVSLSSEIFDLTSGDTPN
                     LRLLTNVYPFRWTEKEPFNWLYLTGGADDILRNSQRDYFVGIGLTFTDNDLKGVIGAA
                     GGAAGAVK"
     gene            complement(5811..>6442)
                     /locus_tag="PKH10_04670"
     CDS             complement(5811..>6442)
                     /locus_tag="PKH10_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT27457.1"
                     /translation="KNLMGLQKPDRGEILYDGRDVVQMNERELLAMRRDIGYLFQEAA
                     LFDSLTVEENVAFPLEEMLRWDDRRAVKERVNEMLELVDLPEAHHKYPAELSGGMRKR
                     VGLARALATGPKIVLFDEPTTGLDPVLSQSIDELILKVNRTLGVTCLIISHDIQAAFH
                     IADTIAFLHDGVIEASGTPREMREARHPVLRLFMENAMDCHDNGTAVRS"
BASE COUNT         1187 a         1908 c         2042 g         1305 t
ORIGIN      
        1 cgtcgggaca tcgtgttcgc tcataagcgc cgcgaatatg ttgacatcga cctcgcggcc
       61 gagcaccgat gcggtgaaca ccccttcctt gagccgctgg gagagccggt cgatgcggct
      121 tatcaggagg gcgttgaccc cctgcggtat ctcgacatcg ccgccgatgg cgtcgaaatg
      181 atcgtcgcgg agttccagca ggttgttgtt cttgagatag aagcagatct gctctacgaa
      241 gaaggggttg ccctcgctcc gctgcatgat gagcgatcgc agattatcgc cgatcggcgc
      301 gccgagcacc gtcttggcga gcagggcgac cgaatcgggc gacagccggt cgagcgggat
      361 ctcggtacgg gcgatccccg gatcggtctc gatgttcgtt ttggtgccgt cgtcctcgta
      421 acggccgagc gtgatgagcg cgaaggggaa ccggtcggtc tgacggaaca ggatcttcac
      481 gatctcgcgc gagtcgttat ccagccactg cacatcgtcg agcgctatcg cgacggggtg
      541 gatgagcgac agcgccttca ggaactcctt gacggcgtac agggtgttct cgaaccggcc
      601 tttcgcgtcg agtttctcgt acaggctgtc cttccactcg atgcctatga gcgcaccgag
      661 gaacgagcgg gtacgggaaa gttcggcgac gacctcctgc gccgagggcg gtatcttggc
      721 gatgatggcg tcccaacgct tctcaaagcg ccgttttttc tcttcgggcg aatcggcgtc
      781 gccctgatcg aagaaccgct cgaagaagtt cacgaagggg ttcatcgatt tgcgaagtat
      841 cgagtctgcc gaaaggacga tgacctcggc cgtcccggcg agccggtcct tcaccgcggc
      901 gaggaaacgg gtcttgccga tgcccggatc gccccagatc atgaggagtc cgccattccg
      961 tccctcggcg agagggccga gcgcggtcgt ggcgcggtcg agttcggcgt cgcgaccgat
     1021 catcgcctgt tcgaaggcga gttcgtcgcc cgccgccttg gggccgcgca gagagaacac
     1081 cggggtcttt tcggtcttcc ccttgaactg gaactcgccg atgaggccgg tctcatactg
     1141 gtcggctatc ttgtcggcca ccatccggga aagccagatg tcgccccacg ccgccttcat
     1201 catgaaccgg gccgatagat tgacccggtc accgagcacc gtgtaggtcg accggcggtc
     1261 gcttccgacg aacccggcgt acacctcgcc ctgcgtgatg ccgcaccgga agggcagttc
     1321 accccgttcc ttgacggcga gtatgaaact gacggcccgt tggacattgt tctcataact
     1381 gaccggtgcg ccgaacagta cgagcatgtt gccgcccttg tcgccgaaat cgagcagatt
     1441 gaaatagccg ccgaacgcgt cgcaggtctc gatgacggcc gcggcgaacc ggtcgagagc
     1501 ctcgaagccg tcgggttgct ggaacgatat gaataccggg acgatatccc ggaattcccc
     1561 ggagagtttc tgggaatata tctgttcggg gtaaaagacg cgaccgacct cgggccgtat
     1621 cgggtcttcg gggcgggaca ccggcgcgga aacggcgtcg gtccacgatg cgatgcggtg
     1681 gaatccggcg gcatcatcgt gcgtcggtcc ggtagagaag gccttttccg gagacttcgc
     1741 gagggcgcga tcgtcgagca ggacgtcgcc ggaacaggcg tggtgctcgg ccgcggcaca
     1801 gccgtctatc gcctcgccgc ggaagaagaa ggtgcgatga tagtcggtcc cggggatacc
     1861 ccagtggaca tccccgaacg atatgccgac ctgcgcgctg atggtgaagg tgccatatgg
     1921 cgtttcgtgg caggatcgga taccgaaaat acgctgtatc tcgcggccgg cgtggcaggg
     1981 gcggaggata tcctcgcccg ggaacaccgc ggtgaaggcg tctccagcga agccggtgat
     2041 gaatccgccg ctctggtaga ccgcgtcgat gaccgggctg aaggtcgaat tgaggatgtc
     2101 cgatatgaac tccgcgccca ctttaccgtg ttgcatgagt ttctcggtca tcgaggtgaa
     2161 gccggaaaca tcgacgaaca tggcgccgcc gcggaacgca ccgtgcagtt ctcccgcgag
     2221 gaaccgttcc tgtatgaagg acgggaacaa cgcgcgcatg ggcggtataa gaccctccgt
     2281 gtcgtgacga tgaccgttga acggtagccg attgttcgtc aaatagcaag tttcatttcg
     2341 gttacgcttg acgggggcgt cccgttcgcg tacaatggct cgacacgaac cggaggagta
     2401 cgccgtggaa ggaacaaaac agcctgataa cagtaagaaa tgcgaactgt cctgcgaggt
     2461 ctggagccgg gtcgtcggct attaccgccc gaccggcaac tggaaccgcg gcaagcagga
     2521 ggaattcaaa aagcggaagc cctttgtcgt gaacgccgtc gctttccccg gcggtcccga
     2581 gccgcacatc gagacgccgc gctgaccgcg ccctcaccgc gccgatgtcc ctgctgaaac
     2641 gatccgccac cgtcctcgtc gggcggatcc tgctttttat cgccatcgcg accttcttct
     2701 ggataccgat gatccgccat tacgccatcc ggtaccgcct gctcggcggc tcgtggtggg
     2761 agcacataca cccggcgcag ttcgactggc tgctcatcgg catcttcttc ttcatgacgg
     2821 cgatctgcgt cttcacgata gatctgcgga agaacctgct gatactcctc atcgcggcgc
     2881 tcgggggact cagcatcgag gggtggggga cccagaccga actctggcgt tactacaccc
     2941 gcgaacggcc gccgctctgg ataatcccgg cgtggccgac cgcggcgctc acgatagatt
     3001 ccctgtcgcg cctcgtagat cgccggatac cgcatatcga cgcgaaatgg tggaacggcg
     3061 cggcgtgggt gttcttcggg tcgttcctcg ccctcatgat cccgttcacc ctgccgtacc
     3121 tgtcctattc gatgacgatc ttttccatcg ccgtcatcgc gctcgtcatc gtctccctct
     3181 gggctggcac cgccgaccga cggaccgcgg tggtcatatt cgtcgtcggg tcggcgctcg
     3241 gctggttcct tgaactgtgg gggacgaccc gacactgctg gatatactac acgggacagg
     3301 aaccgccgct gttcgccgtt ctcgcgcacg gcgccgcgtc ggtgaccttc tggcgggccc
     3361 acctcatcgc cgacggcatc atcacgcggt tgttcccgaa ctatcggggg cactggagcc
     3421 ggggggcggt cgcagagcgg aacggatcac ccatatcccg atgagtgcta ccagtatctc
     3481 gatgacctga ccggcggtga gcagactgaa gactatccgg ctttcctgcc gaacgaactc
     3541 gatgaggaaa ttggcgacgc cgtaaccgac gagacagacc ccggcgatta tgtcgcgccg
     3601 gatgccgcgc cgttccatga cgacgatggc gacgaacagc aggagcatgg aggccgagat
     3661 atatacctgc accggatgga ccgggagcga cggggcgtag cgcgaggcga tctggccgta
     3721 tttgtagtgg agcgccgacg cgtaactgcc gggcgggaac accatgccga tcggcaggtc
     3781 ggtggggcgg ccgaaacagc agccgtcggc cagacagccg agccggatga tggccgagaa
     3841 gagcgggaaa gggtagacga aggggtagaa gaaccgccgc caatccttcc ggatgaacag
     3901 gagtatcgcc gagagggtca gtatgcccgc cgttatcgaa aagcccttga aggcgaactt
     3961 gaagaatttg acgaaaccct cgggggtgta gatgccgaaa atgagttgcg aaacgcccga
     4021 ggtgtagttg aacagttgcc cgttcagcgc cgtctggatg agcagtatcg agagcgggat
     4081 gagcaccgcg ccgacgaatg agaagaagac gatgcggaac aggttccaga attcctttaa
     4141 gaaactgagt tcgcggaccg tcagccatat ccataccgcg aacccgcccc acatgaacac
     4201 attgtaaccg tagccgccga tgtaagggtc gcgcagggtc agccaatcga gcatcgcgcc
     4261 tcgttatttc accgcgcccg ccgcgccgcc cgccgcgccg atgaccccct tgaggtcgtt
     4321 gtcggtgaag gtgagcccga tgccgacgaa ataatcgcgc tgggagttgc gcaggatatc
     4381 gtccgcgccg ccggtcaggt agagccagtt gaacggttcc ttctctgtcc agcggaacgg
     4441 gtagacattg gtcagcagcc gcaggttggg ggtgtcgccg ctcgtgaggt cgaatatctc
     4501 ggaggagagc gatacatact tgaccggcag gtaatccacg ccgacgccgc cggaactctc
     4561 tatgatgccg ctgcggagcg tccagtcacc gaagatgcgg gcgatctgca ggtcgaactt
     4621 gatgccgctc ttctcggtcg tcgtctcgtg ctcggtgacg gtgcggacgg tcggggtctc
     4681 tgcggggtcg gtgatctcgg tgacggtcgt cttctcggtg gtgcgaccgc ccgggtcgtc
     4741 ggtgatgccg atgaggtagt accggtcgtc gcgcggctgg agtttcagcg cgaactggtt
     4801 ctggaaattc tggtcgagcg tccggaactc gctgtggaaa tcgacatgga ccttcatgcc
     4861 gagcgtagag tcggcgtagt cggatgcctt ttcggcgata ttggtcaagt ggtcgtaaag
     4921 tttctcgtcc ttgagcgcct tgccgaggtt cccttcgcca tccttgacgc gccccgttat
     4981 atcctccatg ttcttcatgg tcgccttcgc gctctcaagc gtctcctgcg cgtccttgat
     5041 ctgcccggaa agggcctgca ggctgtcgcg caccgcggcg agcgagtcgc tgatggtctg
     5101 gtcgttgtcg cctatcagtt tgttcgcctt gtcggtgatg gcgatggtgt tcttgatgag
     5161 cagttccatc tgggcgagat tgcggtcgaa gttcgcattc actttgccgg tgatatcggc
     5221 catgttcttg agtgtgatgc gcatcagcac aaggttcgcc tcgacattct gggccgacga
     5281 cctggatacc ttggtcagga catcgatgag ttcggtcatc ttcttctgga aatcctcgcg
     5341 ttcgacgaaa tcgccgatgt tgttgacgat gccttcggcg cgattcgcga tggatttcgc
     5401 cttgcctatg gtgtagttga gtcccgtctg actatccaca tgcgttatcc aatcgccatc
     5461 cttcaagggg ttcgctgtgt cctcgccggc actgagttga agcatggcgg tgccgagcat
     5521 cgactgcatg tccttgtaaa ggaacgcgtc cttgtagagc gccaccttgt cgtatatctc
     5581 gagcgtcacc ttggcgcggt tcccctcgag ttctatggtg cgtatcttgc cgaccgcaac
     5641 gccggcgatg agtatctcgg aatcctccag cagtccggtc gcatcgtcga gataggcgtg
     5701 caatctgtag gtgcccccct tcatgagcca gtcggcgctc ttcatcatgt agtaggaagt
     5761 gagcgccgtg atgacgatga agaagatacc gatcttcgct acccgcgtca tcatgaccgc
     5821 accgccgtgc cgttatcgtg gcagtccatc gcgttctcca tgaaaagtct cagtaccgga
     5881 tgccgcgcct cgcgcatctc acggggcgtg cccgacgcct cgatgacccc gtcgtgcagg
     5941 aacgctatcg tgtcggcgat atggaacgcc gcctgtatgt cgtggctgat gatgaggcag
     6001 gtcaccccga gcgtgcggtt gaccttcagg atgagttcgt cgatgctctg cgagaggacc
     6061 gggtcgagcc cggtcgtcgg ttcgtcgaac agcacgatct tggggccggt ggcgagcgcc
     6121 cgggcaagtc cgacccgttt acgcatgccg ccggagagtt cggcggggta tttgtggtgt
     6181 gcctcgggca gatcgaccag ttccagcatc tcgttcaccc gctctttgac ggcgcggcgg
     6241 tcgtcccagc ggagcatctc ctccagcggg aaggcgacat tctcctcgac agtgagggaa
     6301 tcgaacagcg ccgcttcctg gaaaagatac ccgatatcgc gccgcatggc aagaagttcc
     6361 cgctcgttca tctgcacgac atcccgtccg tcgtagagta tctcgccgcg atcgggtttc
     6421 tggagtccca tgaggttctt ga
//
LOCUS       DAOQUE010000110         6377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_13432_length_6377_cov_4.352578, whole genome shotgun sequence.
ACCESSION   DAOQUE010000110 DAOQUE010000000
VERSION     DAOQUE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13432_length_6377_cov_4.352578"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKH10_04675"
     CDS             complement(<1..288)
                     /locus_tag="PKH10_04675"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HNT27458.1"
                     /translation="MERKTVLETLSVLALAALVFHLWLGHAWLLYLATALLILALFPN
                     PLATLITKGWLKFSELLGAVMSRLIMTLIFFLFLTPLAALYRLFNRAAADHF"
     gene            complement(299..448)
                     /locus_tag="PKH10_04680"
     CDS             complement(299..448)
                     /locus_tag="PKH10_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNT27459.1"
                     /translation="MEFLKDIWGFLKERKKWWLLPMIVVLLLIGVLIVLSSGTAVAPF
                     IYTLF"
     gene            complement(458..2329)
                     /locus_tag="PKH10_04685"
     CDS             complement(458..2329)
                     /locus_tag="PKH10_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HNT27460.1"
                     /translation="MTRYILGISAFYHDSAAALIADGRIIAAAQEERFTRIKQDSHFP
                     LEAAKFCLSYAGVTVSELDAVVFYDKPLLKFERLLETYYTFAPKGLQQFLSFVPVWIK
                     EKLFLKKLIRDALKTLDPTGDPLKVKLLFPEHHLSHAASAFFPSPYEEAAILTIDGVG
                     EWATATIMHGKGNEIQPLKELRFPHSLGLLYSAFTYFLGFKVNSGEYKLMGMAPYGNP
                     SSPKIQEYRDKILTHLIDLRDDGSIWLNPDYFTYTTGLRMIDDKKWAALFGFPTRPVE
                     SHIEQHHCDLAFVIQQLHDEIVLKMAAEAKKLTGAKHLCLAGGSALNCVSNGKLLKSG
                     LFDNIWIQPAAGDAGGALGAALAVHYQYYGTPRTADGVNDAMQGAYLGPEYSELDTEL
                     MARKHKAVYHRIGDFEKLVDLVTDHLTHGKVVGWHQGRMEWGPRALGNRSILGDARNA
                     EMQKKLNLKIKYREGFRPFAPSVIAESSPEFFDLRQGSPYMLLIADVVKERRKTLPEN
                     YHELPLFDKLYMTRSDVQSITHLDFSARIQTVHKETNPRYHRLISRFKEKTGYGVIVN
                     TSFNVRGEPIVCAPEDSFACFMATEIDTLVIGDLLFIKEEQPDWNDREKWKRTFALD"
     gene            2495..3226
                     /locus_tag="PKH10_04690"
     CDS             2495..3226
                     /locus_tag="PKH10_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001921793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNT27461.1"
                     /translation="MKHATTLKLKDLVETYDRVMKVMEHDAAKAIKEDSRAYGGIIRA
                     AKGKMQEYITEELIHIAWDTELAKEAERLTVNSKKIRIPIQMEYVRKIKERTVRDYIL
                     GNIKNYYYGLSVDKHVFIDNKMLLGVECKAYTENAMIKRILVDFHLLKTKFPHLLCYL
                     FQLESQLGGDYSFAVRQPKGSTPTHTLMSYFSDVGLRVVTLLAGERKVDRPINKAQYF
                     KPLTVNRLEVAVEVLIDGFRECGVA"
     gene            3223..4437
                     /locus_tag="PKH10_04695"
     CDS             3223..4437
                     /locus_tag="PKH10_04695"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNT27462.1"
                     /translation="MNIIDLFAGCGGMSLGFHMAGFKSVVANEIDDWAGDTYEHNHPA
                     THLIRKDIREINDWDACLPPSFKARIDGVVGGPPCQGFSLSGNRDARDPRNSLFMEFV
                     RCLKHFMPRFFVMENVPGLLSMRTAQGNSVIEIILSECASAGYKATYSILNAAEYGVP
                     QLRERVFIIGIQKQYPYSKLRIFPQPQVPSEKQITVDLAIGDLPPIESGEGTEFQKYF
                     STPQNAYQEWARHNSAGVYNHVAMRHTQRLIDRFKVIQHGQSVADVPHEHSALKRGDP
                     KVKSGKVYSQNNMRVYGDAPSPTVAASFQSNFIHPHLNRNFTAREGARLQSFPDNYIF
                     KGKRTTMSWEKSLSQYQQIGNAVPPLLAKAIAKNIAQYFEKIDTIADDGKCFGSVQLS
                     LFSEKQEHSSVF"
     gene            complement(4461..4832)
                     /locus_tag="PKH10_04700"
     CDS             complement(4461..4832)
                     /locus_tag="PKH10_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27463.1"
                     /translation="MFGLILAVAAAFLFNLSERPASRSDDARERLLSIFSACAEAAKA
                     KPGPCQLYGKIQYVENFPDVTVKIVENFPDLKVKVVENFADKPGLWKLVQNFPDYKVK
                     VVENFPDIKIKFVENFPGCGK"
     gene            4960..>5909
                     /locus_tag="PKH10_04705"
     CDS             4960..>5909
                     /locus_tag="PKH10_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT27464.1"
                     /translation="MTEQKKTKKASASPKLMKRKSSPAETTIPQSEEKCRIIFDEAAE
                     GIVLMPINGTTLWLNKTFARMHGYDSPEQMAHLRLTDLDAPGTAALAPDRLQRLIAGE
                     TMAFDVEHYRKDGSTFFLNVSCKVIEIEGERYFLGLHQDISERKRAEESLRMAHDSLL
                     AIFEGIDEPVYVADTDTYEVLFVNRVLGSLLGTPGDKKCYEYLQGRHQPCPFCTNEKI
                     LGEYRGRSYVWEFRNEKNRRWYRCIDKAIPWPDGRTVRYEMAIDIDDRKRAEEAIIEN
                     EARLRALTDNIPGVVFQMSYTPEGMLGFQYLSEAAAMLFGP"
     assembly_gap    5910..5919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5942..>6377
                     /locus_tag="PKH10_04710"
     CDS             5942..>6377
                     /locus_tag="PKH10_04710"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT27465.1"
                     /translation="MVYQIDSGTDGSERRFTYLSAGVETLHETTAEEAFADPMRIYRQ
                     VHSDDIPLLAERESEASRDLSTMTAEIRIILPSGDTRWRLFTSAPRRQPDGHLIWDGI
                     ELDITDRKRIEEEREKLREQLAQSQKMESVGRLAGGIAHDFNN"
BASE COUNT         1503 a         1665 c         1807 g         1392 t
ORIGIN      
        1 gaaatggtcg gccgccgcgc ggttgaagag gcggtacagg gccgcgagcg gcgtcagaaa
       61 gaggaagaat atcagcgtca tgataaggcg cgacatgacc gcgccgagca gttcggagaa
      121 tttcagccat cccttcgtga tgagcgtcgc gagcggattg gggaatagcg cgaggatgag
      181 gagcgccgtc gcgaggtaaa ggagccacgc gtgtcccagc cacaggtgga acaccagcgc
      241 cgcgagggcg aggacgctga gcgtttcgag taccgtcttc cgttccaccg tcaactccct
      301 agaagagcgt gtagatgaag ggggcgaccg ccgttccgct cgacagcacg atgagtacgc
      361 cgatcagcag gagcaccacg atcatcggca ggagccacca ttttttccgt tctttgagaa
      421 agccccagat gtccttcagg aactccatcc tatcctccta gtcgagcgca aaggtccgtt
      481 tccatttctc gcggtcgttc cagtcgggct gctcctcctt tataaaaaga aggtcgccga
      541 tgaccagcgt atctatctcg gtcgccatga agcaggcgaa ggaatcttcc ggcgcacaga
      601 ctatcggttc gccgcggaca ttgaaactgg tgttgacgat gacgccgtag ccggtcttct
      661 ccttgaagcg gctgatgagc cggtggtagc gcgggttggt ctccttatgg accgtctgta
      721 tccgcgccga gaagtcgagg tgggtgatcg actggacatc gctgcgggtc atgtacagtt
      781 tgtcgaagag cggcagttcg tggtagttct ccggaagggt cttacgccgc tctttcacca
      841 catcggcgat gaggagcatg tagggcgaac cctgccggag gtcgaagaat tcaggtgaac
      901 tctcggcgat caccgagggg gcgaaggggc ggaacccctc gcggtatttt atcttgagat
      961 tgagtttctt ctgcatctcc gcgttgcgcg cgtcgccgag tatcgaccgg ttgccgagcg
     1021 cccgcggacc ccactccatg cgcccctgat gccagccgac gaccttgccg tgggtcaggt
     1081 gatcggtcac gaggtcgacg agtttctcga agtcgccgat ccggtggtag accgccttgt
     1141 gtttgcgcgc catcagttcg gtgtcgagtt ccgaatattc ggggccgaga taggcgccct
     1201 gcatcgcatc gttcaccccg tcggcggtgc gcggcgtgcc gtagtattga tagtggaccg
     1261 cgagcgccgc gccaagcgca ccacccgcgt cgccggcggc gggctgtatc cagatgttat
     1321 cgaaaaggcc gcttttcagc agtttgccgt tcgatacgca gttgagcgcc gatccgcccg
     1381 cgaggcagag atgcttcgcg ccggtgagtt ttttcgcctc ggccgccatc ttgaggacga
     1441 tctcgtcgtg gagctgctgg atgacgaagg cgaggtcgca gtggtgctgt tcgatgtggc
     1501 tttcgaccgg gcgggtcggg aagccgaaga gcgccgccca ctttttgtcg tcgatcatcc
     1561 gcaggccggt cgtgtaggtg aagtagtcgg ggttcagcca gatggagccg tcgtcgcgca
     1621 ggtcgatgag atgggtcaga attttatcgc ggtactcttg tatcttcggc gatgaggggt
     1681 tgccgtaggg ggccatcccc atcagtttgt attcgcccga gttgaccttg aagccgagga
     1741 agtaagtgaa ggcggagtac agcaggccca gagaatgcgg gaagcggagt tccttgagcg
     1801 gctgtatctc gttgcccttg ccgtgcatga tggtggcggt cgcccattcg ccgacgccgt
     1861 cgatggtgag gatggcggcc tcttcatacg gggaggggaa gaaggccgac gcggcgtggg
     1921 aaaggtggtg ttcggggaag agcagtttta ctttcagtgg atcgccggtg gggtcgagcg
     1981 ttttcagcgc gtcgcggatg agtttcttga ggaagagttt ctcctttatc cagaccggga
     2041 cgaacgagag gaactgttga agtcctttgg gcgcgaaggt gtagtaggtc tccagcaggc
     2101 gctcgaactt caggagcggt ttgtcgtaga agaccaccgc atcgagttcc gacaccgtca
     2161 ccccggcgta ggagaggcag aacttggccg cttcgagcgg gaagtgggaa tcctgcttga
     2221 tgcgggtgaa gcgttcttcc tgcgcggcgg cgatgatgcg gccgtcggcg atgagggcgg
     2281 cggcggagtc gtggtaaaag gctgaaatgc cgaggatgta gcgcgtcatg cgggctccct
     2341 aaagaccgtc gcgactatac tttggtctga ccgccgaggc aagaaaaagg ttgcgtgcgc
     2401 gaaagttgac tttgatttgt cggaaagata aagtgaacag tttttttgga aaagacactt
     2461 gtaaaaatga gaaaacatat gtggaggagt tgaaatgaag cacgccacga cactcaaact
     2521 aaaggacctc gttgaaacct acgacagggt catgaaggta atggaacatg atgccgcaaa
     2581 ggcaatcaaa gaagacagtc gggcgtatgg gggaattatc cgagccgcta aaggaaaaat
     2641 gcaagagtat atcacggaag agctcataca catagcttgg gacacagaac ttgcaaagga
     2701 agcggagaga ctcacggtaa actcgaagaa gatacgaatt cccatacaga tggagtacgt
     2761 tcgcaaaatt aaagaacgca ctgttcgaga ctacatcctt ggaaatatta agaattatta
     2821 ctacggtctt tccgtggaca agcatgtttt tatcgacaac aaaatgctat taggagtaga
     2881 atgcaaagcg tatactgaaa acgcaatgat aaaaaggatt cttgtcgact ttcatcttct
     2941 caaaactaaa tttccccatc ttttgtgtta tctttttcaa cttgaaagcc aacttggagg
     3001 agattattca tttgcagtac gccaacccaa aggaagtacg ccaacccaca cattaatgtc
     3061 atatttctct gatgtaggtc ttcgtgtcgt aacgcttctc gccggtgagc gaaaggtaga
     3121 ccgtccaatc aataaggctc aatattttaa gccgttgacc gttaatcgtc ttgaggttgc
     3181 agttgaagtg ttaatagatg ggtttcgcga gtgcggggta gcatgaacat catagatcta
     3241 tttgctggct gcggcggcat gagccttggg tttcacatgg cggggtttaa atcggttgtt
     3301 gccaatgaga ttgatgactg ggcgggggac acctatgaac ataatcatcc tgcaacccat
     3361 ctcatcagga aagatattcg agaaatcaat gattgggacg cttgtttgcc accatcattt
     3421 aaagctcgaa ttgatggtgt tgtcggtggg cctccttgcc aagggttttc gttaagtggc
     3481 aatcgcgatg cgcgcgaccc aagaaacagt ctctttatgg agtttgttcg atgcctcaaa
     3541 cactttatgc ctcgattctt tgttatggag aatgttcccg gtctgttgtc catgcggacc
     3601 gcgcaaggca attcagtaat cgaaatcatt ctttccgaat gtgcttctgc tggctacaaa
     3661 gcaacgtact ctattcttaa tgccgccgaa tacggcgttc ctcaacttcg cgaacgagtt
     3721 tttattattg gcatccaaaa gcaatacccc tattccaaat taagaatttt tccccagcca
     3781 caagttcctt cagaaaaaca gattactgtt gatctggcga taggtgatct tccgcctatc
     3841 gaatctggcg aaggaacaga gtttcagaaa tatttctcga cacctcaaaa tgcatatcaa
     3901 gaatgggctc gacataattc tgccggtgta tacaatcatg ttgcaatgcg ccacacgcaa
     3961 cgacttatag accgtttcaa ggttattcaa catggccaaa gcgtggcaga tgttccccat
     4021 gaacattccg ctcttaagcg tggagatcca aaagtaaaat caggcaaagt ctattcccag
     4081 aacaatatgc gtgtttatgg ggatgccccg tctcccactg tagcggcttc ttttcaaagc
     4141 aattttattc atcctcattt aaacaggaat tttactgctc gagaaggtgc tcgcctccag
     4201 tcttttcccg acaattacat ctttaagggc aagcgaacca ccatgtcatg ggagaaatcc
     4261 ttatcacagt accaacagat tggaaatgcc gttcctccgt tacttgcaaa agctatagca
     4321 aagaatattg cacagtattt tgagaagatt gacacaattg ccgatgatgg aaagtgtttt
     4381 ggctcggtcc aactttcgct ttttagcgaa aagcaagaac attcctctgt tttctgaaca
     4441 ccagcggaag ttacgaaccc ttacttcccg caccccggga agttctcgac gaacttgatc
     4501 ttgatatcgg ggaagttctc gaccaccttc accttgtagt cggggaagtt ctggacgagt
     4561 ttccagagcc ccggcttgtc ggcgaaattc tcgaccacct tcactttcag gtcgggaaag
     4621 ttctcgacga tcttcaccgt gacatcggga aagttctcga catactggat cttgccgtag
     4681 agttggcacg gccccggctt cgccttcgcc gcctcggcgc acgccgagaa gatggagagc
     4741 agccgctccc gcgcatcgtc cgaccggctg gccggccgct ccgacaggtt gaacaggaac
     4801 gccgccgcga ccgccaggat caaaccgaac atacgaaaca tgatccacct cccttacaga
     4861 ccgttcaact ataatgacgc aacatatcag gggcaagtaa aattgactcg tcttccctcc
     4921 ttggatacac ttcgtcgttc ttttcgggag agtatcctca tgaccgaaca gaaaaaaacg
     4981 aagaaagcat cggcctcccc caagttgatg aagcgaaaat cctcccctgc agaaacgacg
     5041 ataccgcaga gcgaggagaa gtgtcgcatc atcttcgacg aggccgccga aggcatcgtc
     5101 ctgatgccga tcaacgggac gaccttatgg ctgaacaaga ccttcgcgcg gatgcacggc
     5161 tacgacagtc cggaacaaat ggcccacctg cgtctgaccg atctcgacgc ccccggaacg
     5221 gccgctttgg cgcccgatcg actgcaacga ctgatcgcgg gcgagacaat ggccttcgat
     5281 gtcgagcact accgcaagga cggctcgacc ttctttctca atgtctcctg caaggtgatc
     5341 gagatcgaag gtgagcgata ttttctgggc ctgcatcagg acatatccga acgaaaacgg
     5401 gcagaagaat cgctccgcat ggcccacgat tcacttctcg cgatattcga aggcatcgac
     5461 gagccggtct atgtggccga cacggatacc tacgaggtgc tgttcgtcaa ccgggtgctc
     5521 ggatcgcttc tcggcacgcc gggcgataag aaatgctacg aatacctgca gggcaggcac
     5581 cagccgtgtc cgttctgcac caacgaaaag atactcggcg aataccgtgg cagatcgtat
     5641 gtatgggagt tccgcaacga aaagaatcgg cggtggtacc gctgtataga caaggcgatc
     5701 ccgtggcccg acggccgcac ggtccggtac gagatggcca tcgacataga tgaccgcaaa
     5761 agggccgaag aggcgataat cgagaacgag gcgcgcctga gggcgctcac cgacaacatc
     5821 cccggcgtgg tcttccagat gagctacacg cccgagggca tgctcggctt ccagtacctc
     5881 agcgaagcgg cggcgatgct cttcggcccn nnnnnnnnnc agcgacaatc taccgcacgg
     5941 catggtgtat cagatagatt caggcaccga tggttccgag cgtcgtttca cctatctcag
     6001 cgccggtgtc gagacactcc atgaaacgac ggcggaagag gcgttcgccg atccgatgcg
     6061 catctaccgg caggtacatt ccgacgacat ccccctgcta gccgaacggg agagcgaggc
     6121 gtcacgggac ctgtcgacca tgaccgccga gatccgcatt atcctgccat cgggcgacac
     6181 ccgctggcgc ctgttcacct cggccccccg acgccagccc gacgggcatc tgatctggga
     6241 cggcatagaa ctcgacatca ccgaccggaa acggatcgaa gaggagcggg agaaacttcg
     6301 ggaacaactg gcccagtcgc agaagatgga gtcggtgggc cggctggccg gcggcatcgc
     6361 gcacgacttc aacaata
//
LOCUS       DAOQUE010000111         6361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_13482_length_6361_cov_4.108468, whole genome shotgun sequence.
ACCESSION   DAOQUE010000111 DAOQUE010000000
VERSION     DAOQUE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13482_length_6361_cov_4.108468"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKH10_04715"
     CDS             complement(<1..324)
                     /locus_tag="PKH10_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27466.1"
                     /translation="MKRLLLVSLVLLPLVLPAKTFDLEADDEEEKAPATPAPAVTQPQ
                     QPAPATEGSIFVDDTAKTAPVEQQPTAGSEPAPTVTVTEEKKEEPTEEKEEERPLYFS
                     IAAKAD"
     gene            complement(334..1059)
                     /locus_tag="PKH10_04720"
     CDS             complement(334..1059)
                     /locus_tag="PKH10_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNT27467.1"
                     /translation="MRIVACSIVTLLLVACVPPYSPTGEKDPTAPQVATVVPSDGAVG
                     IAPGTVVSVTFTVAIDPTTLTPYSLRLKDDDGHAVIGPLTLSDDGLTASLVPAAPLSE
                     GKTYSVEVTRQIRDDNGIPLDIDGTDSLLLSTFSILATAPTVVSVIPADEATVATDLA
                     TVAVTFSESMDPATITPATFLLSDMQGTVSYDPDTLTARFDIEDPLIPKRDYTIFVAG
                     TVTDAAGIPMGEDVIVRFTTTEE"
     gene            1224..1661
                     /locus_tag="PKH10_04725"
     CDS             1224..1661
                     /locus_tag="PKH10_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27468.1"
                     /translation="MKKKLLVVIAGLFVFSLALSAEETTEPVKEKKQTEEKQKEETKN
                     SGEEQKLQTRERDRERAGQEENAEKKEMKQEQKQERKALKEEQKQEKKEMKQEAKKEM
                     KEGLKEEKKEMKKEQKKDCNEMKKKHKKEQMKGEENPERKPGN"
     gene            1679..2410
                     /locus_tag="PKH10_04730"
     CDS             1679..2410
                     /locus_tag="PKH10_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27469.1"
                     /translation="MKEMFRLTMTILLALVAILAAACSETKTTVPGTDDLTTDETVTD
                     NAVIDETVYDEDATGDKDDVFAEGPLSDDSPMVTDETVTDADTPQTDSILPDDVLTDG
                     EIPDEVMNDELLSDEDVHQGEGGIPDSDQIPVPLTCAEGYVALDITIYHFDENEQKVF
                     GVAGSFTTDPAGQPTTEGDPVQNVCYPVNSTVQIHLTANSGYTFSQWMKPNGSPANDV
                     TGTFPDFSVMLTTKGKRISAEFTVN"
     gene            complement(2426..4384)
                     /locus_tag="PKH10_04735"
     CDS             complement(2426..4384)
                     /locus_tag="PKH10_04735"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNT27470.1"
                     /translation="MSRYPFLLLCAVVFLIACDGTEKVPLKDTDAGSDVDIDADGATE
                     GQLPESDTPLPNDKDIDDTADDVETDGTDEGLDDGETDTDDATGDGLPIDDDIDDTPT
                     DDNKSDEDGDADIAGDGDGPIGPDQDIDTPDDTLDDTVTDILPDTDTEVDIAPDIDAD
                     TDAHICDPETTVECPYSGPSGTKNVGECKAGTKTCDLDGKGWSECAGEVVPIAEICDN
                     GKNDDCDGGTDEQVDEDGDGWFSCVDDCCDSVLACTYPKKVNPGAIEVPEDGIDNDCN
                     GETDENPRTACSSAAKFSGTTALDLINAIDICKTSTGGSWGIVGTPTLTRASGSGTID
                     ASNRQVAVMTQLGTHDSNVAIFPPTDDFINGTMAALSSGRARDANDPDPIAENTYEYY
                     DGTPPADFTAPYAGDLPQTDPDCPAGTGANDGVMLTVDLKVPTNAYGLSFLFRFFSHE
                     YWQFTCNAYNDFFIAMLDTGAAGIPADKNISFDSNGSYISVNSNAFFTVCQPKTGYPC
                     PDGTDPLAGTGWDEQEYDDELGWFYTGGATKWLATAAPVIPGETIKLRFVVWDTSDRK
                     LDSLVLVDNFKWNIEGSDPVDGDPGNDTDILPDGDSDTDVDTDVGPDADSTGPSTYEC
                     WDLNQNGTCDVADEDRSGDGFCTERDCG"
     gene            complement(4477..>6361)
                     /locus_tag="PKH10_04740"
     CDS             complement(4477..>6361)
                     /locus_tag="PKH10_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27471.1"
                     /translation="DSPVMTDDPTTPDEKPDADSDAWEDQDDDGDGIPNGVETPGGVK
                     VDTDNDDTPDYLDDDSDGDGIPDSVEGSGDADGDGVPNYRDTESDGDNILDATECPGQ
                     PCLDTDSDGTPDFLDTDADGDCLDDLYEGVKDPDGDDSPNYLDDDSDGDGILDEDEGC
                     FGGSPLDSDDDGYPNYLDHDSDNDGLSDAKEQELGTDHTLPDTDGDGFDDNTEVAAGS
                     DPLVKDPEFYEGQFYVVLPYQDPEKNDALKFSTQIKKVDLVITLDLSGSMSYARDNLK
                     TGINTTVVPGVAAAVPDPAFGLSSFAGIEDAAFFLNQPVTKDANAMTTAVENLGGAGG
                     GKVEAQYENLYQIATGTGFNGELLEVSLDFSEWVEDWYEHYADVVIPAVDCTGQLGSI
                     GGACFRPAALPIVMMITDENLYDINDDWVSDGGVLRIRYKWRDSKDQGHYASEVIAAM
                     NAKKAKFIGVVGEGVGDLLFDITEQYEEIADGTGSKGTNGQYFIFQTGYSGNGLSSEI
                     AAAVKELVENIAMDVTTTRTSVGNPYSIDTTQFIVDIIPSYSDPADSYASKDPTTFYQ
                     VKPGINIFFDVTFKNDIFEPTTTENTLFRAHIQVLGEGALLDERDVFIIVPGIKDDGG
                     IES"
BASE COUNT         1210 a         1777 c         2060 g         1314 t
ORIGIN      
        1 gtcggctttg gcggcgatgc tgaagtagag tggccgttcc tcctccttct cttcggtcgg
       61 ctcctctttt ttctcttcgg tgacggtcac ggtcggggcc ggctcggacc ccgcggtcgg
      121 ttgttgttcg accggcgcgg tctttgcggt gtcgtcgacg aagatcgatc cctccgttgc
      181 cggagctggt tgttgtggtt gggtaacggc cggagccggt gttgcgggtg ccttttcttc
      241 ctcgtcgtcg gcctcaagat cgaaggtctt cgccgggagc acaagcggga gaagcaccag
      301 cgatacgagc agtagtctct tcatgttccc ctcctactct tccgtcgtgg tgaagcggac
      361 gataacatct tctcccatcg ggatccctgc cgcatcggtc acggtgccgg cgacaaagat
      421 cgtgtagtcg cgtttgggta tcagcgggtc ctcgatgtcg aatcgggcgg tcagggtatc
      481 gggatcgtag gagacggttc cctgcatatc gctgagcagg aaggttgccg gggtgatggt
      541 ggccgggtcc attgattcgg agaaagtgac cgcgaccgtt gcaaggtcgg tggccaccgt
      601 cgcttcgtcg gcggggatca ccgaaacgac cgttggcgcg gtcgcgagga tggaaaaggt
      661 tgataggagc aaggagtcgg tcccgtcgat gtcgagcggt atgccgttgt cgtcccgtat
      721 ctgtcgcgtc acctcgacgg agtaggtctt tccctccgac aagggcgccg cgggtaccag
      781 acttgccgtc aggccgtcgt ccgatagggt gagtggaccg atcaccgcat gcccatcatc
      841 gtcctttagg cgcaaagagt aaggggtaag ggtcgtcggg tctatcgcga cggtgaaggt
      901 gaccgaaacg acggtgccgg gcgcgatgcc cacggcgccg tccgagggga caacggtcgc
      961 cacttgcggc gcagtcgggt ccttctcgcc ggtcggcgag tagggtggga cacacgcgac
     1021 aagtaaaagg gtcacaatac tgcatgcgac gatcctcatg gtgatcctcc cgaactggat
     1081 gtgaaccaaa tatatcgtag gataccggag agcaggaaaa agttccacaa aaaataggaa
     1141 tttcttgtgg aacttttatg cttcgccggt cgtcatacgg gcgtgatgag attccgccgt
     1201 gtcatgatga gaaggaggag accatgaaga agaagttgct cgttgttatc gctgggcttt
     1261 ttgttttttc tctggcgctt tcggccgagg agacgaccga gccggtgaag gaaaagaagc
     1321 agaccgagga gaagcagaag gaagagacca agaattcggg ggaggagcag aaactccaga
     1381 cccgcgaacg ggaccgtgag cgggccggac aggaagagaa cgccgaaaag aaagagatga
     1441 aacaggagca gaaacaggag agaaaggctc tgaaggaaga gcagaaacag gaaaagaaag
     1501 agatgaaaca ggaagccaag aaagaaatga aggaggggct caaggaagag aagaaggaga
     1561 tgaagaaaga gcagaagaag gactgcaacg agatgaagaa gaagcacaag aaggaacaga
     1621 tgaaaggcga agagaacccc gagcgcaagc cgggtaacta gaaaaaagga ggaggtcgat
     1681 gaaagaaatg ttccgcctga cgatgacgat cctgctggcg ctggtcgcga tactggccgc
     1741 cgcctgtagc gaaacgaaga ccacggtgcc gggcaccgat gatctgacga ccgatgagac
     1801 cgttaccgac aacgcggtga tcgacgagac cgtgtacgac gaggacgcca ccggcgacaa
     1861 ggacgatgtg ttcgccgagg gaccgctctc cgacgacagc ccgatggtga ccgatgagac
     1921 ggtgaccgat gccgatacgc cgcagaccga cagcatcctg cccgacgatg tcctgaccga
     1981 cggagagatc cccgacgagg tcatgaacga cgagctcctt tccgacgagg atgtgcatca
     2041 gggcgaggga ggcatacctg acagcgacca gataccggtg ccgctcacct gtgcggaagg
     2101 gtatgtcgcg ctcgatatca ccatctatca tttcgatgag aacgagcaga aggtgttcgg
     2161 cgtggccggc tccttcacca ccgatcccgc cggtcagccg acgaccgaag gggaccccgt
     2221 gcagaatgtc tgctatccgg tgaactcgac cgtgcagata cacctgacgg ctaacagcgg
     2281 gtacaccttc agccagtgga tgaaaccgaa cggcagtccc gcgaacgatg tcacgggcac
     2341 cttccccgat ttttccgtga tgctcacgac caaaggcaag cggatatccg ccgaattcac
     2401 cgtcaactga ccttttcccg caacttcacc cgcagtcgcg ttcagtacag aatccgtcgc
     2461 cgctccgatc ctcatcggcg acatcgcagg tgccattctg gttcaggtcc cagcactcgt
     2521 aggtggaggg gccggtcgaa tcggcgtcgg gcccgacatc ggtatcaaca tcggtatcgc
     2581 tgtcaccgtc gggcaggata tcggtgtcgt taccggggtc gccatcgacc ggatcggaac
     2641 cctcgatgtt ccacttgaaa ttatcgacga gcaccagcga atcaagtttg cggtccgagg
     2701 tgtcccagac gacgaagcgg agttttatcg tctctccggg gatgaccggg gcggcggtcg
     2761 cgagccattt ggtggcaccg ccggtataga accagccaag ttcgtcgtcg tattcctgct
     2821 cgtcccagcc ggtcccggcg agaggatccg tgccgtcggg gcagggatag ccggttttcg
     2881 gttggcatac ggtgaaaaag gcgttcgagt tgacgctgat ataggagccg ttgctgtcga
     2941 acgatatgtt cttgtcggct gggatgcccg ccgcgccggt gtcgagcatc gcgatgaaga
     3001 aatcgttgta ggcgttacag gtgaactgcc agtattcgtg agagaagaaa cggaacagga
     3061 acgagagtcc gtaggcgttg gtcggcacct tcaggtcgac ggtgagcatc accccgtcgt
     3121 tcgcaccggt gccggccggg cagtcgggat cggtctgcgg gagatcgccg gcgtaggggg
     3181 cggtgaagtc ggcgggcggg gtaccgtcgt agtattcgta tgtgttctcg gcgatcggat
     3241 cggggtcgtt cgcatcgcgg gcgcgccccg aagaaagcgc agccatggtg ccgttaataa
     3301 agtcgtcggt cggggggaat atggcgacat ttgagtcgtg ggtgcccaac tgggtcatca
     3361 ccgcgacctg tcggttgctg gcgtctatgg tcccggagcc actcgcgcgg gtgagggtcg
     3421 gggtgccgac gataccccac gagccgcccg tcgaggtctt gcagatatcg atcgcattga
     3481 tcagatcgag cgcggtggtg ccgctgaact tcgcggcgga agagcaggcc gtgcgcgggt
     3541 tctcgtcggt ctcgccgttg cagtcgttgt cgatgccgtc ctcgggcacc tcgatcgcgc
     3601 cggggttgac ctttttcgga taggtgcagg cgagcacgct gtcacagcag tcgtcgacgc
     3661 aggagaacca gccatcgccg tcctcgtcca cctgttcgtc ggtgccgccg tcgcaatcgt
     3721 cgttcttgcc gttgtcgcag atctcggcga tagggaccac ctcgcccgcg cattcgctcc
     3781 atcccttgcc gtcgagatcg caggtcttgg tccccgcctt gcattcgccg acattcttgg
     3841 tgcccgaagg tccggagtag ggacactcga ccgtggtctc cgggtcgcat atatgggcat
     3901 ccgtatcagc atcgatgtcg ggcgcgatgt ccacttcggt gtccgtatcg ggtagtatgt
     3961 cggtcaccgt gtcgtcgagc gtatcgtcgg gcgtgtcgat atcttggtcg ggaccgatcg
     4021 gtccgtcccc gtccccggcg atatccgcat cgccatcttc atcgctctta ttgtcatcgg
     4081 tcggggtatc gtcgatgtcg tcgtcgatgg ggagtccgtc gccggtcgcg tcatccgtat
     4141 cggtctcccc gtcgtcgaga ccctcgtccg ttccgtcggt ctcgacatca tccgcagtgt
     4201 cgtcgatatc cttgtcgttc ggcagaggcg tgtccgattc ggggagttgc ccctcggtcg
     4261 caccgtcggc atcgatgtcg acatcagagc cggcgtcggt atccttcaat ggtacctttt
     4321 ccgtcccgtc acaggctatc aaaaagacga ccgcgcacag caaaagaaag ggataacggg
     4381 acatgggcct ctccctcggc gatggatgaa ccgatggttt ataccacatt cggggcggta
     4441 ttgcaacaag aaatgtcagt ggatacgggg gaatggttac gattcgatgc cgccgtcgtc
     4501 cttgatgccc gggacgatga tgaaaacatc gcgctcgtcg aggagtgccc cttcgccgag
     4561 cacctgaatg tgtgcccgga acagcgtgtt ctcggtggtg gtcggctcga agatatcgtt
     4621 cttgaaggtg acatcgaaga agatgttgat ccccggtttc acttgataga aggtcgtcgg
     4681 gtccttactc gcgtaactgt cggccgggtc gctgtaggag gggatgatgt ccacgatgaa
     4741 ctgggtcgta tctatgctgt aggggttgcc gaccgaggtg cgggtggtgg tgacatccat
     4801 cgcgatgttc tcgacgagtt ccttgaccgc cgccgctatt tcggaggaga ggccgttgcc
     4861 cgaataaccg gtctggaaga tgaagtactg gccgttggta ccctttgatc cggtgccgtc
     4921 tgctatctcc tcatactgtt cggtgatgtc gaacagcagg tcgccgaccc cctcgcctac
     4981 cacgccgatg aacttcgcct ttttcgcgtt catcgcggcg atgacctcgc tcgcgtaatg
     5041 tccctgatcc ttgctgtcgc gccatttgta gcgtatgcgc aggacaccgc cgtcgctcac
     5101 ccagtcgtcg ttgatgtcgt agaggttctc gtcggtgatc atcatgacga tcggaagcgc
     5161 cgcggggcgg aaacaggcgc caccgatgga gcctaactgc ccggtgcagt cgaccgccgg
     5221 tatgacgaca tcggcatagt gttcatacca atcctccacc cattcgctga aatcgagcga
     5281 cacctcgagc agttctccgt tgaagcccgt gccggtcgct atctggtaaa ggttctcata
     5341 ctgggcctcg accttgcccc ctcccgcacc gccgagattc tcgaccgcgg tggtcatcgc
     5401 gttcgcgtcc ttggtgaccg gctggttgag gaagaaggcg gcgtcctcga tgccggcgaa
     5461 cgacgaaagc ccgaacgcgg ggtcggggac cgccgcggcg accccgggga ccacggtggt
     5521 gttgatgccg gtcttgaggt tgtcgcgggc gtaactcatc gatcccgaca ggtcgagcgt
     5581 tatcacgaga tcgaccttct ttatctgggt gctgaatttc agcgcgtcgt tcttctcggg
     5641 gtcctgatag gggagcacca catagaactg accctcgtag aattcggggt ccttcacgag
     5701 cgggtcggat ccggccgcga cctcggtgtt atcgtcgaaa ccgtcgccgt cggtgtcagg
     5761 tagcgtgtga tcggtgccga gttcctgctc cttcgcgtcg gagaggccgt cgttgtcgct
     5821 gtcgtggtcg agatagttcg ggtagccgtc atcatcgctg tcgagagggg aaccgccgaa
     5881 acagccctcg tcctcgtcga gtatcccgtc gccgtccgag tcgtcgtcga gatagttggg
     5941 gctatcatcg ccgtcggggt ccttgacgcc ttcgtagaga tcgtcaagac agtcgccgtc
     6001 ggcgtcggta tcgaggaaat cgggtgtgcc gtcgctgtcg gtgtcgagac agggctgacc
     6061 gggacattcc gtcgcgtcga ggatattgtc gccgtcactt tcggtgtcgc ggtagttggg
     6121 gacgccgtcc ccgtcggcgt cgccggatcc ctcgacgctg tcggggatgc cgtcaccgtc
     6181 cgagtcgtca tcgagatagt cgggggtgtc gtcgttgtcg gtgtcgacct tgaccccgcc
     6241 gggcgtctcg acgccgttcg ggatgccgtc gccgtcatcg tcctgatcct cccacgcgtc
     6301 gctgtcggca tcgggcttct cgtccggtgt cgtcggatcg tcggtcatca ccggtgagtc
     6361 g
//
LOCUS       DAOQUE010000112         6279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_13732_length_6279_cov_4.415006, whole genome shotgun sequence.
ACCESSION   DAOQUE010000112 DAOQUE010000000
VERSION     DAOQUE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13732_length_6279_cov_4.415006"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKH10_04745"
     CDS             complement(<1..376)
                     /locus_tag="PKH10_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT27472.1"
                     /translation="MTYHPKVVGKKCYLSPIRAEDAETYTRWMNDLEVQKNLIIPVPI
                     ITLDSEKEWIANIGKREDTVIFAIVEKKGNTLIGNAGLHQINLRNRSAEFGIVIGEKK
                     FWGKGFGTEATNLVLDYGFNVLN"
     gene            515..1489
                     /locus_tag="PKH10_04750"
     CDS             515..1489
                     /locus_tag="PKH10_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27473.1"
                     /translation="MRQLSFLPLFALIFLAACQEDTASRDKAAQSVMVKQTAAAMVEE
                     CPNGGVALDIGFDKNGNGTLDTEEILKSEVICNGKDGEPGQPGEKGDTGAKGATGATG
                     EKGDTGDQGLSGLDGAAGLPCWDLNGDGIEDIFEDVNSDLAWNALDCTGPQGETGTKG
                     DKGDPGEKGDKGDTGDQGEPGLSATGGWGSAYGNATAIFAKNGDLAWNAVGPMRGVTI
                     DTDKKIFTVTEAGVYHITYRLTVLKPMGLKRWASVALIVNGVEQTAARRSVAGGGEIA
                     TTALLDLPAGAMVTLRNVGATDLFPVDPPGGAGDPSADAPAIVFIKLN"
     gene            1501..2718
                     /locus_tag="PKH10_04755"
     CDS             1501..2718
                     /locus_tag="PKH10_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27474.1"
                     /translation="MLKHIMTLSLLSVLCAAAPLSAVESFRAPYTTVCVNSDGVITCE
                     EKTIQEIPLTYGEPVQIEVSYTNTCDATSCSTETPVLFTLTKSLPQDISPLTFLHTVR
                     TLSALPAAQNGTANSPYAAYRAYTVGAARRDYVVNVTLSQGATVLDTFDISAKEPRRL
                     MIDDDHPVLARLLDETRSPLTDDTPAGGLLFVPVKQDGTLEPVYEGLLLPQAVAADIV
                     DARQLNQLRDADLAKIATDPNAETAYLVRYQRAFRNAVATMGSSLMLVREVPDRAFST
                     ARLELDRAVVGGVRREAVGWIQTAGVEPFEAMSKNGRLSVTVENVGSVTAAYMVSVLD
                     CSSFVGVPIITRFVDVAAGAQSNFVFEIPLSGDTADQQACHVMLQSLTGRVFDDVELF
                     FTPSATDTTRVQY"
     gene            complement(2761..3129)
                     /locus_tag="PKH10_04760"
     CDS             complement(2761..3129)
                     /locus_tag="PKH10_04760"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT27475.1"
                     /translation="MTKKENKNQQPKAEIVEPIDPRPESELTLSFRTTDPIPYNFFPG
                     IARDPATGGLFVALDRPFPVGTRLNLSFLTESRRVESIAEVCWSHKLYNGSTPETKVR
                     LVDIDPEDRDLIEFYIREKK"
     gene            complement(3221..3754)
                     /locus_tag="PKH10_04765"
     CDS             complement(3221..3754)
                     /locus_tag="PKH10_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27476.1"
                     /translation="MKNPILFMPLLAALFLFVSCAGADAPGGGAALTTEDIAGIWEAG
                     PREFTKTCQGDDEIEEGEGSYLSIVALDATQVVIDLCYDRDCAEMEDDPRTLPFQGNR
                     AVYGPPGVYLVYDTPDCEIYMEIFRYDISFSSASTAVHRQHFKIMYEGTRCEDEEGVK
                     LEDCETEFSSDMWKIHG"
     gene            complement(3765..4967)
                     /locus_tag="PKH10_04770"
     CDS             complement(3765..4967)
                     /locus_tag="PKH10_04770"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT27477.1"
                     /translation="MKAFLVPLFLLTWAFFGCSSNGTGEENDAVDDGDDVPVIDDPTA
                     NEDASLNDTSLTDDQAILPDEDVPEDDADPPPDTLPTADQDTVSPADDGPDDDLLADG
                     EETDDDAGEENDDATTGDDTDQTGTLTEGEQKFPGAPADMGREGDFTVLDPLGNGETI
                     VVDAATRLVWQQNVAYNDTHTAAMAWGYCISLTYAGYKDWDLPTVHELATIYSYGTTA
                     FEYDEFFTGYSEGFWTDTIDHDNRRWVLYGTGKAEPAPSISDLLYARCVRRTEVPIFT
                     GVRFTYEKGSDNKTIIVDHLTGLQWTPYIYYDSIMWGGALTTCENTTHGGYDDWRLPG
                     VNEMRSIIDYGKYNPASNADYGYGSVIWTSAAYHVGERWIVDIKDGSQSPWESDGAFN
                     VLCVRDPE"
     gene            complement(5047..5562)
                     /locus_tag="PKH10_04775"
     CDS             complement(5047..5562)
                     /locus_tag="PKH10_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27478.1"
                     /translation="MLSLPADIFVEMPGQLAPFETPFDAPGLPAARCRFIFLGNRTKG
                     RYSSLLPFDYDFAALGWGLADYFPMGYANLYHIEKIRAFHPDPAQAPQARTDNRREPP
                     LYRPKGLGSRPEEGALYYVFRAPIVTEWGRDIIGLSFSEKRAMAMAERYVETYGRVAL
                     VGLLLVKVTWH"
     gene            complement(5566..5847)
                     /locus_tag="PKH10_04780"
     CDS             complement(5566..5847)
                     /locus_tag="PKH10_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNT27479.1"
                     /translation="MIRHVVMWKLLDAADAPRAKEALLALRGKIPLVRSLEVGVDVLR
                     TERSYDLCLIATFDSLADLAAYTDHPAHREVVAFMQKIREKAVAVDFEA"
     gene            complement(5844..>6279)
                     /locus_tag="PKH10_04785"
     CDS             complement(5844..>6279)
                     /locus_tag="PKH10_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT27480.1"
                     /translation="RLSGRRVIVQAPGSLLGRIPSDATLFPLARAPHDILFPRCSAIV
                     HHGGAGTTQTAVRAGRPSVVVPHLMDQFFWGDLLYRRGMAPPPIRRRRLTVEKLSHAI
                     RQAASDPAMSERAATIGAQVAAEDGVARAVELIEAHIKGGSR"
BASE COUNT         1211 a         1934 c         1896 g         1238 t
ORIGIN      
        1 ggttgaggac attgaacccg tagtcgagca cgaggttggt cgcctcggtc ccgaaccctt
       61 tgccccagaa cttcttctcg ccgatgacga tgccgaactc ggcggagcgg ttccgcaggt
      121 ttatctggtg aagccccgca ttgccgatga gggtgttccc tttcttctcg acgatggcga
      181 agatgaccgt gtcctcccgc ttgccgatat tggctatcca ctccttctcc gaatcgagcg
      241 tgatgatcgg caccgggatg atgaggttct tctgcacctc gaggtcgttc atccagcgcg
      301 tataagtctc ggcatcctcg gcgcggatgg gtgaaagata gcatttcttc ccgacaactt
      361 tgggatggta ggtcatgaca gcctccgtaa gtgatgcttc aggatacctc cggcaggaac
      421 atgaagcaag aaaatcagtt ctccctacct tctctttcct tgactcggaa cgccccgcac
      481 atacaatgca ccaccctttt tcggaggcct tttcatgcga caattatcgt ttcttcccct
      541 gttcgctctc atcttcctcg ccgcctgtca ggaagatacc gcctcgcgcg acaaggccgc
      601 gcaaagcgtc atggtaaaac agaccgccgc agcaatggtc gaagagtgtc ccaacggcgg
      661 ggtcgcgctc gatatcgggt tcgacaagaa tggcaacgga acgctcgata ccgaagagat
      721 actgaagagc gaggtcatct gcaacggcaa ggacggcgag ccgggccagc caggcgaaaa
      781 aggcgacacc ggcgcgaagg gggcgaccgg cgctaccggc gaaaaaggcg acaccggcga
      841 tcaaggtctc tccggccttg acggcgccgc cggactcccc tgctgggacc tgaacggcga
      901 tggcatcgag gatatcttcg aggatgtgaa cagcgacctt gcgtggaacg ccctcgactg
      961 caccggcccg cagggcgaga ccgggaccaa aggcgacaag ggcgatcccg gcgaaaaggg
     1021 cgacaaagga gataccggcg atcagggcga accgggactt tcggcgaccg gcggctgggg
     1081 aagcgcctac ggcaacgcga ccgctatctt tgcgaagaac ggcgatcttg cgtggaacgc
     1141 ggtcggcccg atgcgggggg tgaccatcga cacggacaag aagatattta ccgtgaccga
     1201 ggcgggcgtc taccacatca cctaccggct gacggtcttg aaaccgatgg gattgaaacg
     1261 gtgggcatcg gtcgcgctta tcgtcaacgg cgtggagcag acagcggcgc ggcggtcggt
     1321 cgcggggggc ggcgagatcg cgacgacggc gctgttggac ctccccgccg gggcgatggt
     1381 gacactgcgc aatgtcggag caacggacct gttcccggtc gatcccccgg gtggagccgg
     1441 cgatccttcc gccgacgccc cggccatcgt cttcatcaaa ctgaactgag ggagggatcg
     1501 atgttgaaac atatcatgac tctgtcgctc ctctctgtcc tgtgcgccgc agcgccgctc
     1561 tccgcggtcg aatctttccg cgcgccctat accaccgttt gcgtcaattc cgacggcgtt
     1621 atcacttgcg aagaaaaaac cattcaggag ataccgctca cctatggtga gccggtacag
     1681 atcgaggtca gctataccaa tacctgtgac gccacttcct gttcaacgga aacgcccgtt
     1741 cttttcacgc tcaccaaaag tctgccgcag gacatctcgc cgctcacctt cctccatacg
     1801 gtgcggaccc tctcggcgct cccggcggcg cagaacggca cggcgaattc cccctacgcc
     1861 gcataccgcg cctacacggt cggggcggcg cgacgcgact atgtggtgaa tgtaacgctc
     1921 tctcagggcg ccacggtgct tgacaccttc gatatctccg cgaaggaacc gcgccgcctc
     1981 atgatagatg atgatcatcc ggtccttgcg cggcttctcg atgaaacgcg ttctcccctt
     2041 acggacgata cgccggccgg cggcctcctc ttcgttccgg tgaaacagga cggtacgctc
     2101 gaaccggtgt acgaagggct ccttctgccg caggcggtag ccgccgatat cgtcgatgcg
     2161 cgacaactca accagttgcg cgacgccgat ctggcgaaga tcgcgacgga tccgaacgcc
     2221 gaaacggcct atctggtgcg gtatcaacgg gcgttcagga atgcggtcgc cacgatgggc
     2281 tcttcgctca tgctcgtgcg cgaggttcct gaccgtgcct tcagcaccgc gcgcctcgaa
     2341 ctcgaccgcg cagttgtcgg cggcgttcgg agggaggcgg tcggatggat ccaaaccgca
     2401 ggggtggaac cctttgaggc gatgagtaag aacggacggc tttcggtcac ggtcgagaat
     2461 gtgggctccg tgacggccgc gtatatggtc tccgtgctcg attgctcctc gtttgtcggc
     2521 gtaccgatca tcactcggtt cgtcgatgtt gcggcgggcg ctcaaagcaa cttcgttttc
     2581 gagatcccgc tgagcggcga caccgccgac cagcaggcgt gccatgtcat gcttcagtct
     2641 ttgacgggca gagtgttcga cgacgttgaa ctcttcttta cgccgagtgc caccgacacc
     2701 acgcgcgttc agtattgaag agattcttcc atcagttcag tgtcgtgggg ggtgcgggaa
     2761 ttatttcttc tcgcggatgt agaactctat caggtcgcgg tcctcggggt caatgtcgac
     2821 gagacgcacc ttcgtttcgg gggtggagcc gttgtacaac ttgtgactcc agcagacctc
     2881 ggcgatgctc tcgacgcggc gcgattcggt gaggaaggag agattcagtc tcgtgccgac
     2941 cgggaacggg cgatcgagcg cgacgaaaag tccgccggtg gcggggtcgc gggcgatgcc
     3001 ggggaagaag ttatacggga tggggtcggt ggtacggaag gagagggtca gttcgctctc
     3061 cggacgcggg tctataggtt ccacgatctc cgctttcggc tgctggttct tgttttcttt
     3121 cttcgtcatg aggccactcc cttatcatgg tcgtcgggtg accataccgc cgtgaaagtg
     3181 aaaagcaaga ggacggcgaa taattttttg ccgtcaccct tcaaccgtgt atcttccaca
     3241 tatcggagct gaattcggtc tcgcagtcct ctagtttcac cccttcctca tcctcgcacc
     3301 gggtcccctc gtacatgatc ttgaaatgct ggcggtggac cgccgttgag gcgctcgaaa
     3361 aggagatatc ataccggaat atctccatgt atatctcgca gtcgggcgtg tcgtagacca
     3421 gatagacgcc gggcggaccg tacacggccc ggttgccttg gaacgggagc gtccgggggt
     3481 catcctccat ctcggcgcag tcgcggtcat agcagaggtc aatgaccacc tgtgtcgcgt
     3541 ccaacgcaac gatgctgaga taagaaccct ccccttcctc tatttcgtca tcgccctgac
     3601 aggtcttggt gaactcgcgc gggcccgcct cccatatccc cgcgatgtct tcggtcgtaa
     3661 gcgcggcgcc gccacccggg gcgtccgccc ccgcgcagga aacgaagagg aacaacgccg
     3721 caaggagcgg catgaaaagg atcgggtttt tcatggcact ccccctactc cgggtccctg
     3781 acgcacagga cattgaacgc gccgtcgctt tcccacggcg attgcgaccc gtccttgata
     3841 tccactatcc aacgctcgcc gacatggtac gcggcggagg tccatatcac gctgccataa
     3901 ccgtaatcgg cattcgatgc ggggttatat tttccataat cgatgatgct ccgcatctcg
     3961 ttgacccccg ggagccgcca gtcatcatag ccaccgtgcg tggtgttctc gcaggtcgtc
     4021 agcgcgccgc cccacatgat ggaatcgtag tatatgtagg gcgtccactg aaggccggtc
     4081 agatggtcga cgatgatcgt cttgttgtcg ctccccttct cgtaggtgaa acgcaccccg
     4141 gtgaagatcg ggacctccgt ccgtctgacg caccgcgcat acaggaggtc gctgatcgag
     4201 ggggcaggct ccgcctttcc ggtgccatag agcacccatc gacgattgtc gtgatcgatc
     4261 gtatcggtcc agaacccctc tgaatagccc gtgaagaact cgtcgtactc gaacgcggtc
     4321 gtgccgtaac tgtaaatggt cgccaactcg tggaccgtgg gcagatccca gtccttgtag
     4381 cccgcgtagg tcagcgaaat gcagtagccc cacgccatcg cagcggtgtg ggtgtcgtta
     4441 tatgccacat tttgttgcca tacgagtcgg gtcgcggcat cgaccactat cgtctcgccg
     4501 ttgccgagag gatcgagcac cgtgaaatca ccctcgcgtc ccatatcggc cggggctccc
     4561 gggaactttt gttcgccttc ggtcagcgta ccggtctggt ccgtatcgtc cccggtcgtc
     4621 gcgtcatcgt tctcctcccc cgcatcgtca tccgtttctt caccgtcggc gaggaggtca
     4681 tcgtcggggc cgtcgtctgc cggtgacacc gtgtcttgat cagcggtcgg cagggtatcc
     4741 gggggaggat ccgcatcgtc ttccggcaca tcctcatccg ggaggatcgc ctggtcatcg
     4801 gtgagcgatg tgtcgttcag cgaagcgtcc tcattggcgg tcggatcatc gatgacgggg
     4861 acatcgtcac catcgtcgac cgcatcgttt tcctcgccgg tcccgttact tgaacaaccg
     4921 aagaatgccc atgtgagcaa gaacaggggg acaagaaacg ctttcacgga gtgttccctc
     4981 ctcatataga ccgtacccga tagtaacccg cccgaatgac gaatcaagaa attcctgtga
     5041 aggattctaa tgccaggtca ctttgacaag aagcagtccc acaagcgcga cacgaccata
     5101 ggtctcaaca taccgttcgg ccatcgccat cgcccgcttt tcgctgaacg acagccctat
     5161 gatgtcgcgg ccccattcgg tcacgatcgg ggcacggaac acatagtaga gcgccccttc
     5221 ctcggggcgg gagccgagcc ccttcggacg gtagagtggc ggttcgcggc ggttgtcggt
     5281 ccgtgcctgc ggcgcctgcg ccggatcggg atggaacgcc cgtatcttct cgatatggta
     5341 gagattggcg tagcccatcg ggaaatagtc ggcgagtccc cagccgagcg ccgcaaagtc
     5401 gtagtcgaat ggcagaagcg agctgtaccg ccctttcgtg cggttcccga gaaagatgaa
     5461 gcgacagcgc gccgccggga gaccgggggc atcgaagggg gtctcgaagg gtgccaactg
     5521 ccccggcatc tcgacgaaga tatcagccgg taacgagagc atgcctcacg cctcgaagtc
     5581 gaccgcgacc gctttttcgc gtatcttctg catgaacgcg accacctcgc ggtgggccgg
     5641 atggtcagtg tatgccgcga ggtccgcaag cgaatcgaag gtggcgatga gacaaaggtc
     5701 gtaagaacgc tcggtccgca acacatcgac gccgacctcc agcgaccgga cgagcggtat
     5761 cttcccccgg agcgcgagga gtgcctcctt tgcccgtggg gcatcggcgg catcgagcag
     5821 tttccacatc accacatgac ggatcatctc gaacctccct tgatatgtgc ctctatcagt
     5881 tccacggcgc gggcgacccc gtcctcggcg gcgacctgcg ctccgatggt cgcggcccgt
     5941 tcactcatcg ccggatcgct tgccgcctgc cggatggcgt gcgacagttt ttcgaccgtc
     6001 agccggcgac gacggatcgg cggcggcgcc atgccgcgcc ggtacaaaag gtcgccccag
     6061 aagaactggt ccatcaggtg gggcaccacg accgagggac gaccggcgcg aacagccgtc
     6121 tgtgtcgtcc ccgccccgcc gtgatgcacg atggcggaac agcgggggaa aaggatatcg
     6181 tgaggtgcgc gggcaagcgg aaaaagcgtc gcatccgacg ggatgcgccc cagaagcgat
     6241 cccggcgcct gcacgataac acgccgcccc gacagccgc
//
LOCUS       DAOQUE010000113         6244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_13842_length_6244_cov_3.818387, whole genome shotgun sequence.
ACCESSION   DAOQUE010000113 DAOQUE010000000
VERSION     DAOQUE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13842_length_6244_cov_3.818387"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKH10_04790"
     CDS             complement(<1..264)
                     /locus_tag="PKH10_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27481.1"
                     /translation="MTAFLEVLDTFKPKKNARAHAVELLTDIARRDSISPEAVLEKVG
                     ASELLVRPGHPLENFDELVKRLKALRYPLWTSKKERLNALCARL"
     gene            complement(264..1412)
                     /locus_tag="PKH10_04795"
     CDS             complement(264..1412)
                     /locus_tag="PKH10_04795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNT27482.1"
                     /translation="MKTYDILVIGGGSVGVPVALYLAQKGKKVAVVEKHASVGRGQNR
                     AAIGGIRATHSDPAKIKICQMSLDIVRKMEHEQGLHIDWHQGGYLFPVYDTERERSLK
                     NLLEVQHKFGLNIDWIDAEEVQRLCPGINPRGLRGGTYSPEDGFASPLKTAGAFYKLA
                     CDAGVDFYFYETALSFEKKDTKIVSLKTDKNTFSAGLIINAAGGDARKIGQLVGADLP
                     VYPDSHEGGITEPVQRFFEPMIVDIRSDGHSDNYYFYQAVGGQVIFCITPNPKHYGKD
                     IDNTSEFLPLCVRRMLELYPRLRNIRVRRTWRGLYPMTPDGFPIVGYTKETDNMLHLV
                     GMCGQGYMLGPGLGKVIAETICDNSKDYKFIFDQLSPYRAFTGTEMLK"
     gene            complement(1414..3483)
                     /locus_tag="PKH10_04800"
     CDS             complement(1414..3483)
                     /locus_tag="PKH10_04800"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT27483.1"
                     /translation="MGRIQEHPVLETKKETRQVSFTFDGAKMTGIDGEMISSALFAAG
                     ITRFSLHRKDGAPQGIFCANGQCSQCTVIVDGHAQKSCVTPLRDGMDIRTLDRLPALP
                     ADDEPLRNYEKKVVNCDVLVVGGGPSGLTAAIELAKLGLRIVLADDKDRLGGKLVLQT
                     HKFFGSEEDCYAGTRGYEIAKILEKELRSYKNVTIYPETSVVGIYKDRKAGLFVENRR
                     YLIVSFEGLIVSAGARERSIVFPGNDLPGVYGAGAFQTLVNRDLVRAAKKVLIVGSGN
                     VGLIAAYHALQAGINVAGIVEIAGEISGYKVHADKIRRMGVPIYLSTTVLSAAGNGTV
                     ERVTVAEVDKQWNPLLETARTFAVDTLLIAAGLTPADEFYDMAEHFEFKVVKAGDADE
                     IAEASSAMFGGRIAGLKMAKMLGEDVVIDDTWLKKAEVLKSKPGEVFPLDPVTLSDKF
                     KPIFTCLEEIPCNPCTTVCPKSAIKLKDKIGTIMDVPYLAGECIGCGICVAACPGLAI
                     TLARKVDEKTAEVMLPHEYIASFKVGDKVTLTDIDGKEIEKAEVVKLQQNKKYRTTLV
                     TVRMSVKNAAIAAGIRVQDEKETRPLPEPVLDHIPDNGIVCRCERVTLGEVVKFIKEH
                     KVRDINQLKQIRVGMGACGSKTCSVQFPRVFRAAGVDFSTVAEGSIRPLSVETPMSAL
                     INETEGE"
     gene            3634..4716
                     /locus_tag="PKH10_04805"
     CDS             3634..4716
                     /locus_tag="PKH10_04805"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNT27484.1"
                     /translation="MERKFRLVNDDHVYTFEEIAEKITNGGLLKSAYIWEPSLTDWVK
                     LKDYPAFAQIFSAYEAQAEESMKKALGTDDEAMERKQTRQLLSDVSKEEQKLAFQQRP
                     VVRWAITALACVAMLGSAIKVMYTLFQTKEGQELLGTVVEELPPMETIDLERVRMASG
                     VTKLDEVKGIAVKKVEKSREKELLAEILADIKREEAQEQAQAAAGQAAAKQEPPKKRA
                     GLFDKVSDEEIEQFRRSLLAKNAAVGSRKEIGKAGGAEKPVEVQEELSSKQISKVVKE
                     HSSSTIAYCYNRSLKLDASLSGKLEVTVSVLGSGRVAKVETNTQKFKGTNLEQCVKDM
                     IKKKWTFPQFNGTITEVTIPFLLSSE"
     gene            complement(4734..>6244)
                     /locus_tag="PKH10_04810"
     CDS             complement(4734..>6244)
                     /locus_tag="PKH10_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNT27485.1"
                     /translation="VDTILLNLGFEKSDWDRPANTLSGGWQMRLALARVLVREPDVIL
                     LDEPTNYLDIATIDFLASWLLNFEGQALLVSHDRDFLNRVVEETWEVYGGSVGIYRGN
                     YDYYVVEKEQRVAVLAKQREKQLDEIRGLQDFVDKNRYNAATAALAQSKLKQLEKIKG
                     SLIVLPRTPPTMTFRLPPPKRGGEIVAEMQGLCHRFGDLKVIDDYKRIITRRERIALL
                     GRNGFGKSTLMNILGGEIEPTAGACALGKDIEVSYFRQHEITLLPPDMTVIRFVESIA
                     GFDMMGKVKTLLGCFLFYEDDWDKKIAVLSGGEKVRLAFIRMILSPGNLLLLDEPTTH
                     LDIDSKEVLLRTLQDIDATIVFVSHDSHFINSLATSVVYFRGRCDMVNFPGTYQEYLN
                     MYGHDIIFDEAEDRRPVEGPVSKGKVDHALRKELRNKLNKLKKDIESTQKEIAKREEE
                     KMALAAKLAQGVGDIALQSKRLARLEEELLSLMSRWEESSAAFEKLSKESDG"
BASE COUNT         1155 a         1825 c         1962 g         1302 t
ORIGIN      
        1 caggcgcgcg cagagagcgt tcagccgctc ctttttcgag gtccagagcg gatagcggag
       61 cgccttcagc cgtttcacca gttcgtcgaa gttctccaac ggatgaccgg gcctgacgag
      121 cagttccgaa gcgcccacct tctccagcac cgcctcgggg ctgatgctgt cgcggcgcgc
      181 aatatcggtc agcaattcta cggcatgggc acgggcgttc tttttcggct tgaatgtgtc
      241 gagcacttcg aggaacgcgg tcattatttg agcatctcgg tgccggtgaa ggcgcggtag
      301 ggggagagct ggtcgaagat gaatttgtag tccttgctgt tgtcgcagat ggtctcggcg
      361 atcaccttgc cgagccccgg cccgagcatg tagccctgcc cgcacatccc gacgaggtgg
      421 agcatgttgt cggtctcctt ggtgtaaccc actatcggga agccgtcggg cgtcatcggg
      481 tagaggccgc gccaggtgcg gcggacgcgg atgttgcgga gccgcgggta gagttccaac
      541 atccgacgga cgcagagcgg caggaactcg gaggtgttgt cgatgtcctt gccgtagtgc
      601 ttcgggttgg gggtgatgca gaagatcacc tgcccgccga ccgcctgata gaagtagtag
      661 ttgtcgctat ggccatcgga gcggatatcg acgatcatcg gctcgaagaa gcgctggacc
      721 ggttcggtga tgcctccttc gtggctgtcg ggatagaccg gtagatcggc gccgaccagt
      781 tgcccgatct tgcgcgcgtc gccaccggcg gcgttgatga tgagacccgc cgagaaggtg
      841 ttcttgtcgg tcttgagcga gactatcttg gtgtccttct tctcgaacga gagggccgtc
      901 tcgtagaagt agaaatcgac cccggcgtcg caggcgagtt tgtagaaggc gccggcggtc
      961 ttaagcggcg aggcgaagcc gtcctcgggc gaataggtgc cgccccgcag gccgcgcgga
     1021 ttgatgccgg ggcataagcg ctggacctct tcggcatcta tccagtcgat gttgaggccg
     1081 aatttgtgct ggacctcgag caggttcttg agcgaccgct cccgttcggt gtcgtagacc
     1141 gggaacagat agccaccctg atgccagtcg atgtggagcc cctgttcgtg ctccattttg
     1201 cggacgatgt cgagcgacat ctggcatatc tttatcttgg ccggatcgct gtgggtggcg
     1261 cggatgccgc cgatcgcggc gcggttctgg ccgcggccga ccgacgcgtg tttctcgacg
     1321 accgccacct tcttcccttt ctgggcgaga tagagcgcca ccggtacgcc gaccgaacca
     1381 ccgccgatga cgaggatatc gtaggtcttc atgtcattct ccctccgtct cgttgatgag
     1441 ggcggacatc ggtgtttcga ccgacagcgg ccggatggac ccttcggcga cggtcgagaa
     1501 gtcgaccccc gccgcccgga agacgcgcgg gaactggacc gaacaggtct tcgagccgca
     1561 ggcccccatg ccgacccgta tctgtttgag ctggttgatg tcgcgtacct tgtgctcctt
     1621 gatgaacttc accacctcgc cgagcgtcac ccgctcgcag cggcagacga tgccgttgtc
     1681 gggaatatgg tcgagcaccg gttcgggaag cggccgggtc tctttttcat cctgcacccg
     1741 gatgccggcg gcgatagcgg cattcttcac gctcatgcgg acggtgacca gcgtcgtgcg
     1801 gtatttcttg ttctgctgga gtttgaccac ctcggccttt tcgatctcct ttccgtcgat
     1861 gtcggtgagc gtcactttgt cgccgacctt gaacgatgcg atgtattcgt gcggcagcat
     1921 cacctcggcc gtcttttcgt ccactttccg ggcgagcgtt atcgccagcc ccggacaggc
     1981 cgccacgcag atgccgcagc cgatgcactc gccggcgagg taggggacat ccatgatggt
     2041 gccgatcttg tccttaagtt ttatcgcgct tttcgggcag acggtggtgc agggattgca
     2101 ggggatctct tcgagacagg tgaatatcgg cttgaattta tcggagagcg tcaccggatc
     2161 gagcgggaag acctcgccgg gcttgctttt gagcacctcg gcctttttaa gccatgtatc
     2221 gtcgatgacc acatcctcgc cgagcatctt ggccatcttg agccccgcga tgcggccgcc
     2281 gaacatggcg ctcgacgcct cggcgatctc gtcggcgtcg cccgctttca ccaccttgaa
     2341 ctcgaaatgt tcggccatgt cgtagaattc gtcggccggg gtgagccccg ccgcgatgag
     2401 gagcgtatcg accgcgaagg tgcgcgccgt ctcgaggagc gggttccact gtttatcgac
     2461 ctcggcgacc gtcacccgtt cgaccgtgcc gttcccggcg gccgacagga ccgtggtgct
     2521 caggtatatc ggcaccccca tgcgccgaat tttatcggcg tggaccttgt agccgcttat
     2581 ctcgcccgct atctcgacga tgccggcgac attgatgccc gcctgcaggg cgtggtaggc
     2641 ggcgatgagg ccgacattgc ccgagccgac gatgagcacc ttcttggccg cccgcaccag
     2701 atcgcggttc acgagcgtct ggaaggcgcc cgcgccgtag acgccgggaa ggtcgttgcc
     2761 ggggaagacg atcgatctct cccgcgcccc ggccgacacg atgagccctt cgaaactgac
     2821 gatcaggtaa cgtcggttct cgacaaagag tcccgcctta cggtctttgt agatgcctac
     2881 caccgaggtc tcggggtaga tggtcacatt tttatatgag cgcagttctt tttcgagtat
     2941 cttggcgatc tcgtagccgc gtgtcccggc gtaacagtcc tcctccgagc cgaagaattt
     3001 gtgggtctgg agcaccagtt tgccgccgag ccggtccttg tcgtcggcca gcacgatacg
     3061 cagaccgagt ttcgccagtt cgatggccgc ggtgagcccc gagggaccac cgccgaccac
     3121 cagcacatcg cagttgacca ccttcttctc atagttgcgc agcggttcat cgtcggccgg
     3181 gagcgccggg agtcggtcga gcgtccggat atccatccca tcgcgcagcg gtgtgacgca
     3241 cgatttctgg gcatgaccgt cgacgatgac ggtgcactgc gagcactggc cgttggcgca
     3301 gaatatcccc tgcggcgctc cgtccttgcg gtggagcgag aagcgggtga tgccggcggc
     3361 gaagagcgcc gagctgatca tctcaccatc gatgccggtc atctttgcgc cgtcgaaggt
     3421 gaacgatacc tgccgtgttt cctttttcgt ttcgagtacc ggatgttcct gaatgcgacc
     3481 catgatatat ctcctcggaa agcattacct aatgccgaat gtaaacctat gcgccaccgc
     3541 tcaaaagtca ataaaagggc tgtgcggacg atttgtttga catcggcaac gaaatcaggc
     3601 aaaatcatta cactttgcct gcgaggatga ccgatggaac ggaagttcag actggtcaac
     3661 gacgaccatg tctacacctt cgaggagatc gccgaaaaga taacgaacgg tggacttctc
     3721 aagagcgcct acatctggga accgtctctc accgactggg taaaactgaa ggactatccc
     3781 gcctttgcgc agatattctc cgcctacgag gcgcaggccg aggagtcgat gaaaaaggcg
     3841 ctcggtaccg acgacgaggc gatggagcgc aagcagacgc gacaactgct ctccgatgtt
     3901 tcaaaggagg aacagaagct cgctttccag cagcggcccg ttgtgcggtg ggccatcacc
     3961 gcgctcgcct gtgtagcgat gctgggatca gcgatcaagg tgatgtacac gctgttccag
     4021 accaaggaag gacaggaact gctcggtacc gtcgtcgaag aattaccccc catggagacg
     4081 atagaccttg aacgggtccg catggcgtcg ggtgtcacga aacttgacga ggtgaagggg
     4141 atagcggtca aaaaggtcga aaagagccgc gagaaggaac tgctcgccga gatactcgcc
     4201 gacatcaaga gagaggaggc gcaggaacag gcgcaggccg ccgccggtca ggccgcggcg
     4261 aagcaggagc ccccgaagaa aagggcggga ttgttcgaca aggtatccga cgaggagatc
     4321 gagcaattcc gccggtcgct cctcgccaag aacgcagcgg tcggctcacg caaggagatc
     4381 ggtaaggcgg gtggcgccga aaagccggtc gaggtacagg aggaactgtc gtccaaacag
     4441 atatccaagg tggtcaagga gcattcgagc agcactatcg cctactgtta caaccgttcg
     4501 ctcaaactcg atgcgtcgct gtcgggcaaa ctcgaggtga ccgtcagcgt cctcgggagc
     4561 ggtcgggtcg ccaaggtcga aacgaacacg cagaagttca aggggaccaa cctcgaacaa
     4621 tgcgtcaagg acatgataaa gaagaaatgg accttcccgc agttcaacgg caccatcacc
     4681 gaggtgacga taccgttcct gctctcttcg gaatagttcc cgtccccttc ggcctacccg
     4741 tcgctctctt tgctgagttt ttcgaacgcg gcggagcttt cctcccaccg gctcatcagg
     4801 gagagcagtt cctcttcgag ccgggcgagc cgtttgctct ggagcgcgat gtcgccgaca
     4861 ccctgcgcga gtttcgccgc cagcgccatc ttttcctctt cgcgcttggc gatctccttt
     4921 tgggtactct ctatatcttt ctttagttta ttcaatttgt tgcgcagttc cttgcgcagg
     4981 gcgtgatcca cctttccctt cgagaccggt ccttcgaccg gtcgccggtc ctcggcctcg
     5041 tcgaagatga tgtcgtggcc gtacatgttc aggtattcct gataggtgcc ggggaagttg
     5101 accatgtcgc accggccgcg gaaatagacg accgaggtgg cgagagagtt gatgaaatgg
     5161 gaatcgtgcg agacgaagac gatggtggcg tcgatatcct gcagggttcg caggagcacc
     5221 tccttcgagt cgatatcgag gtgggtggtc ggctcgtcga ggagcaggag gttgccgggg
     5281 ctcagtatca tccggatgaa ggcgagccgc accttttcgc cgccgctcag caccgcgatc
     5341 ttcttatccc aatcgtcctc gtagaagagg aaacagccga gaagcgtctt cactttcccc
     5401 atcatgtcga acccggcgat ggattcgacg aagcggatga ccgtcatgtc ggggggcagg
     5461 agcgttatct cgtgctggcg gaagtaactt acctcgatgt ccttgccgag cgcgcaggcg
     5521 ccggcggtcg gctctatttc gccgccgagg atgttcatga gcgtcgattt gccgaacccg
     5581 ttgcggccga ggagcgctat ccgttcccgc cgcgtgatga tgcgtttgta gtcgtcgatc
     5641 accttgaggt cgccgaagcg gtggcagagt ccctgcatct cggcgactat ctcgccgccc
     5701 cgcttgggcg gcggcaggcg gaaggtcatc gtcggcgggg tgcgcggcag gacgatgagc
     5761 gaccccttta tcttttcgag ttgcttgagt ttcgactggg cgagcgcggc ggtggcggcg
     5821 ttgtatcggt tcttgtcgac gaaatcctgc aggccgcgta tctcgtcgag ttgtttctcg
     5881 cgctgtttgg cgagcaccgc gacccgttgc tccttttcga cgacatagta gtcgtagttg
     5941 ccgcggtaga tgccgaccga gccgccgtag acctcccagg tctcctcgac cacgcggttg
     6001 aggaaatcgc ggtcatgcga gacgaggagc gcctgtccct cgaagttgag gagccacgag
     6061 gcgaggaagt ctatcgtggc gatgtcgaga tagttggtcg gctcgtcgag gagtatcaca
     6121 tcgggctcgc ggacgagcac ccgggcgagc gccagccgca tctgccagcc gccgctcagc
     6181 gtgttggcgg ggcgatccca gtcgcttttc tcgaagccga gattgagcag tatcgtgtcg
     6241 accg
//
LOCUS       DAOQUE010000114         6234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_13876_length_6234_cov_4.903059, whole genome shotgun sequence.
ACCESSION   DAOQUE010000114 DAOQUE010000000
VERSION     DAOQUE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13876_length_6234_cov_4.903059"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>336)
                     /locus_tag="PKH10_04815"
     CDS             complement(<1..>336)
                     /locus_tag="PKH10_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27486.1"
                     /translation="HYTTAGGWESPADCAWSCDTDYALEGGTCINSKEVQCDEDNANP
                     VNSSDVIANVTITYTSAGGWAAPADCAWSCDTDFALEGGACINQKDVPCDTDNANPLN
                     SSDTIVNVT"
     assembly_gap    337..436
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<437..2882)
                     /locus_tag="PKH10_04820"
     CDS             complement(<437..2882)
                     /locus_tag="PKH10_04820"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNT27487.1"
                     /translation="MVRFRFLTMMLALLWCSGSLLADMILVRDGGDTRVNGTYTYNGL
                     YNGRDSYIYTSESNIFTWHLRWEPTTLCSANCWSIVDPSTGYIYYYSTAQQGNVPTAS
                     WKENAPYGIAPSVRVDVVASALKYNRTQLNESSLNNGSINGSITITLESSTIDFTGTV
                     DENYIDNGKASVTGTPAGLTAVITLVDTTHVSLSFTGSAPPPHDTDVTVTVAFANEAF
                     SNNKRSDVGLADGAPVRLNFIRKITVCKPSGCDYDTIAAAIAAAGEGDIIDIGEGTWG
                     ESGLTIDESLIFEGAGADETVIDAGDAGQISLVADTTMDLIFRGIHFRNGDAGDDLGG
                     AISFAGVTATNTLLIEDCKFSNNSANYGGGAVYVSKATTTIRRTLFFGNEASGATSHG
                     GALHIDQADTYIENSTFTGNIAAYTGGAIYVSALSVLTLMNDTIDANTSTSDLGGGIA
                     TTEGTIGSSNTIIAENIGYDIYAFTSITLNLSYSIYQETACSTSCTVVGSGTDSNNQV
                     DDPDVAPLAMNGGTTETMALLTGSPAIDAGTSASAPTDDQRKYTRDINKDIGAYEKMP
                     TQRIECTNIPVNAHGINNNADGKFEQTWNGDFWEPTTFTCEWACDTDFALEGGACINQ
                     KDVPCDTDNANPLNSSDTIVNVTIHYTTAGGWESPADCAWSCDTDYALEGGTCINSKE
                     VQCDEDNANPVNSSDVIANVTITYTSAGGWAAPADCAWSCDTDFALEGGACINQKDVP
                     CDTDNANPLNSSDTIVNVTIHYTTAGGWESPADCAWDCDIDYALEGGLCINSKEVQCD
                     TDNANPLNSSDTIVNVT"
     gene            complement(3022..4239)
                     /gene="lysA"
                     /locus_tag="PKH10_04825"
     CDS             complement(3022..4239)
                     /gene="lysA"
                     /locus_tag="PKH10_04825"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNT27488.1"
                     /translation="MNMIGGCDLRDIANRFGTPLYLYDAARIERNCHALRDAFNAHYP
                     ATDIFYAVKANGNPHLLRLIASCGTGADCAAPLEVTLAERAGIPREKIMYTGNYESRD
                     DLAAAARCGAVNLDDITAIGKLPAGFDGILSFRINPGEGGGSHPGIVTGGPDAKFGIP
                     IESAPAAYRLAVDRGFTRFGIHLMAGSNNRDPLFFARMAAILCHSAADIFRPLGILPE
                     YFDIGGGFGIPYAEDETPLDLDAMARAVAATLALCASQHGVTPPRLRIEPGRFIVGDA
                     GYLLARVTAVKKGLRRFVGLDAGMNTLIRPALYGSHHRVTVVGKETPAGMATLCGRIC
                     ENTDIFAADIPFPAVTEGDIVLFHDAGAYCASMAFPYNGRLRPAEVLCDNGKPFRIAR
                     AENEAEFMERYEA"
     gene            complement(4236..5582)
                     /gene="lysC"
                     /locus_tag="PKH10_04830"
     CDS             complement(4236..5582)
                     /gene="lysC"
                     /locus_tag="PKH10_04830"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HNT27489.1"
                     /translation="MKVIKFGGTSVGTADAIRQVEKIVRNAGPGSLTVVSAFSCITNA
                     LTRTITLMRQGNAPEALAELDGVEERHRAMIAELGLPAATACFTADTVDRVRGLIPAL
                     TALGDVTPRAADTILATGETLSSRIIVDHFALQGLDATHIDAREVLITDASFGAAQPD
                     TALIEEAVRCKLLPLLTRHRAVVAGGYIGATPQGVTTTLGRGGSDYSAALLAAAIGAD
                     ECQIWTDVDGIMTCDPRIIPAARTVPRLTYDEAAELAFFGAKVLHPLTIFPAVRKGIP
                     VVIRNTFAPDHPGTRVIAQNGNDRRLKAIAFQRGITVLHIRSNRMLGAYGFLAAVFTV
                     FRDHRTPVDLVTTSEVSISVTIDDTAHLDAITVDLMRLGEVSVEQGRAIVSLIGDGMR
                     ETAGIGARFFNTLRTVNVGMVSVGASEVNISIVIAERDLEEAVRLIHNEFFDGGAA"
     regulatory      complement(5661..5832)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5957..>6234
                     /locus_tag="PKH10_04835"
     CDS             5957..>6234
                     /locus_tag="PKH10_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27490.1"
                     /translation="MKGTGVTALLFLCLLLAVSCGDDGTKTIPDNAQVPDESAVTDDT
                     VTDDTVVDDTVTDDTVTDDTVADDTITDDTVIDDTVTDDTVVDNETPD"
BASE COUNT         1116 a         1730 c         2025 g         1263 t
ORIGIN      
        1 tcgtcacatt gacgatggtg tcggagctgt tgagcgggtt ggcgttgtcg gtgtcacacg
       61 gcacatcctt ctggttgatg caggcgccgc cttcgagggc gaaatcggtg tcgcagctcc
      121 atgcacagtc ggcgggggcg gcccaaccgc ctgctgaagt gtaggtgatc gtcacattgg
      181 caatgacatc ggagctgttg accgggttgg cgttgtcctc atcacactgc acttccttcg
      241 agttgatgca ggtgccgcct tccagcgcgt agtcggtgtc gcaactccac gcacagtcgg
      301 ccggtgactc ccaaccgcct gcggtggtat agtggannnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnntcgt cacattgacg atggtgtcgg agctgttgag cgggttggcg
      481 ttgtcggtgt cgcactgcac ttccttcgag ttgatgcaga ggcctccttc cagtgcgtag
      541 tcgatgtcgc agtcccatgc acagtcggcc ggtgactccc aaccgcctgc ggtggtatag
      601 tggatcgtca cattgacgat ggtgtcggag ctgttgagcg ggttggcgtt gtcggtgtca
      661 cacggcacat ccttctggtt gatgcaggcg ccgccttcga gggcgaaatc ggtgtcgcag
      721 ctccatgcac agtcggcggg ggcggcccaa ccgcctgctg aagtgtaggt gatcgtcaca
      781 ttggcaatga catcggagct gttgaccggg ttggcgttgt cctcatcaca ctgcacttcc
      841 ttcgagttga tgcaggtgcc gccttccagc gcgtagtcgg tgtcgcaact ccacgcacag
      901 tcggccggtg actcccaacc gcctgcggtg gtatagtgga tcgtcacatt gacgatggtg
      961 tcggagctgt tgagcgggtt ggcgttgtcg gtgtcacacg gcacatcctt ctggttgatg
     1021 caggcgccgc cttcgagggc gaaatcggtg tcgcaggccc attcgcaagt gaaggtggtc
     1081 ggttcccaaa agtcgccgtt ccaggtctgt tcaaacttgc cgtcggcgtt gttgttgatg
     1141 ccatgtgcat tgaccgggat gttcgtgcat tcgatacgct gggtcggcat cttctcgtaa
     1201 gcgccgatgt ccttgttgat gtcgcgggta tatttccgct gatcatcggt gggggcgctg
     1261 gcggaggtgc cggcatctat ggcgggactg ccggtcagga gtgccatcgt ctcggtcgtg
     1321 ccgccgttca tggcgagcgg ggccacatcg gggtcatcga cctgattgtt gctgtcggtg
     1381 cccgaaccga cgacggtgca ggaggtgctg caggcggtct cctggtaaat ggagtaggaa
     1441 aggttgagtg ttatcgaggt gaaggcgtag atatcgtagc cgatgttctc ggcgatgatg
     1501 gtgttgctcg aaccgatcgt tccttcggtc gtggcgatgc cgccgcccag atcggaagtg
     1561 gaggtgttcg catctatcgt gtcgttcatg agggtcagga ccgaaagggc gctgacatag
     1621 atcgcgcctc cggtgtaggc cgcgatattt ccggtgaagg ttgagttctc gatataggtg
     1681 tccgcctgat cgatatggag tgctccgccg tgggatgtcg ctccggatgc ttcattgccg
     1741 aagaaaaggg tgcgccggat ggtggtggtc gccttcgata catagacggc gccgccaccg
     1801 tagttcgcgc tattgttcga gaatttacag tcctcgataa gcaatgtgtt ggtggcggtc
     1861 acgccggcga acgatatcgc gccaccgagg tcgtcaccgg catcgccgtt gcggaagtgt
     1921 atgccgcgga agataagatc catcgttgta tcggccacga gggatatctg accggcgtca
     1981 ccggcatcga tgaccgtctc gtctgcccct gctccttcga agatgaggct ttcgtcgatg
     2041 gtcagtccac tctcgcccca tgttccttca ccgatatcga tgatgtcgcc ctctcccgcc
     2101 gccgcgatgg cggcggcgat ggtgtcatag tcacagccgc tcggcttgca gacagttatc
     2161 ttccggatga aattcagccg gaccggtgcg ccgtcggcga gcccgacatc ggatctttta
     2221 ttgttgctga aggcctcgtt ggcgaaagcg accgtgacgg tgacatcggt gtcgtggggg
     2281 ggcggtgcgc tgccggtgaa ggagagggaa acatgggtgg tgtcgaccag cgttatcacg
     2341 gcagtaaggc ctgccggcgt gccggtcacg ctcgccttgc cgttgtcgat gtaattctcg
     2401 tcgacagtgc cggtgaagtc gatggttgac gactcgagcg tgatggtgat agaaccgttt
     2461 atggagccgt tattcaggga cgattcgttg agttgggtgc ggttgtactt gagcgccgag
     2521 gcgaccacat cgacgcggac cgacggggcg atgccgtagg gggcgttttc cttccaactg
     2581 gccgtaggaa cattgccctg ttgggccgtg gagtagtagt agatatagcc ggtgctcgga
     2641 tcaacgatcg accagcagtt ggctgagcag agagtcgtcg gctcccaccg aagatgccag
     2701 gtgaagatgt tcgattcaga ggtgtagatg taagaatccc taccgttgta gagcccgttg
     2761 taggtgtagg taccgttcac ccgcgtgtcg ccgccgtcgc ggacgaggat catgtcggcg
     2821 agcagggatc cactacacca caaaagcgcg agcatcatgg tcaaaaacct gaaccgcacc
     2881 ataacaacct ccacctacgg taatttgcca cataagaaaa gcatcggcgt gacccacgca
     2941 aaaaacagac cagtgtatca tgcttataaa aagagtcaat agccctaata taattataat
     3001 gatttcgggt ggtatgaaag gttatgcttc gtagcgttcc atgaattccg cctcattttc
     3061 ggcccgggcg atacggaagg gtttcccatt atcgcacagc acctcggcgg gtcgtagacg
     3121 gccgttgtag gggaaggcca tggatgcgca gtaggcgccg gcgtcgtgaa agaggacgat
     3181 atcgccttcg gtgacggcgg ggaaggggat gtcggcagcg aagatgtcgg tgttctcgca
     3241 gatgcgaccg cagagcgtcg ccatgcccgc aggggtctcc ttgccgacga ccgtcacgcg
     3301 gtggtgtgag ccatagagcg ccgggcggat gagggtgttc atcccggcgt cgagtccgac
     3361 gaaccggcga agtccctttt tgaccgccgt gacgcgggca agaagatatc cggcgtcgcc
     3421 gacgatgaag cgccccggtt cgatgcggag ccgtggcggc gtgacgccgt gctgtgaggc
     3481 gcagagcgcg agcgtcgccg ccaccgcccg tgccatcgcg tcgagatcga gcggcgtttc
     3541 gtcctcggcg taggggatgc cgaacccgcc gcctatgtcg aaatattccg gcaggatgcc
     3601 gagcggtcgg aagatgtcgg cggcgctatg gcagaggatg gcggccatcc gcgcgaagaa
     3661 gagagggtcg cggttgttcg atccggccat gagatggatg ccgaaccggg taaagccgcg
     3721 gtcgaccgcg agccgatagg cggccggcgc gctctctatc ggtatgccga acttcgcgtc
     3781 gggtccgccg gtgacgatac cgggatgaga gccgcctcct tcgcccgggt tgatgcggaa
     3841 cgagagaatg ccgtcgaacc cggcgggcag tttcccgatc gccgtgatat cgtcgaggtt
     3901 caccgcgccg caccgcgcgg cggcggcaag gtcatcgcgt gattcgtagt taccggtgta
     3961 catgatcttc tcgcgcggta tgccggcccg ctcggcgagc gtcacctcga gcggcgcggc
     4021 gcagtcggcg ccggttccgc acgaggcgat gagccgcaaa aggtgcgggt tgccgttcgc
     4081 cttgaccgcg taaaagatgt cggtcgccgg ataatgcgcg ttgaatgcgt cacgcagggc
     4141 atgacaattc cgttcgatgc gcgcggcgtc gtatagatag agcggcgtgc cgaaacggtt
     4201 cgcgatatcg cgcaggtcgc atccgccgat catgttcatg cggcgcctcc gtcgaaaaat
     4261 tcgttgtgta tcaatcgcac cgcctcttca agatccctct cggcgatgac gatcgatatg
     4321 ttgacctcgc ttgccccgac gctcaccatg ccgacattga cggtgcgcag ggtgttgaag
     4381 aagcgggcgc cgatcccggc cgtttcgcgc atgccgtcgc cgatgagcga cacgatggcg
     4441 cgcccctgtt cgaccgacac ctctccgagt cgcatgagat cgaccgttat cgcatcgagg
     4501 tgtgcggtgt cgtcgatggt caccgatatg ctcacctccg aagtggtgac gagatccacc
     4561 ggcgtgcggt gatcgcggaa cacagtaaaa acggcggcaa ggaacccgta ggcgccgagc
     4621 atccggttgc tgcggatgtg gagcaccgtg atgccgcgct ggaaggcgat cgccttgaga
     4681 cgccggtcgt taccgttctg tgcgatgacc cgtgtgccgg gatggtcggg agcgaaggtg
     4741 ttgcggatga cgaccgggat gcccttccgg accgccggga atatcgtgag cgggtggagc
     4801 accttcgcgc cgaagaaggc gagttcggcc gcctcgtcgt aggtgagccg cggtacggtg
     4861 cgagccgccg ggatgatgcg gggatcgcag gtcatgatgc catcgacatc ggtccatatc
     4921 tgacattcat cggcaccgat cgccgccgca agaagcgctg ccgaatagtc ggacccgccg
     4981 cgtccgagcg tggtggtcac cccctgcggc gtcgcgccga tgtagccgcc ggcgaccacg
     5041 gcgcgatgtc gcgtgagcag cggcaggagt ttgcagcgta ctgcctcttc gatgagcgcc
     5101 gtgtcgggct gggccgcgcc gaacgaggcg tcggtgatga gcacctcgcg ggcgtcgata
     5161 tgcgtcgcgt cgagtccctg caacgcgaaa tggtcaacta tgatgcgcga ggagagtgtt
     5221 tcccccgtcg cgagtatggt gtcggcggcg cgcggagtga catcgcccag tgccgtaagc
     5281 gccgggatga gaccgcggac gcggtcgacg gtatcggccg tgaaacaggc cgttgcggcg
     5341 ggaaggccca gctcggcgat catcgcgcgg tgccgctctt cgaccccgtc gagttccgcg
     5401 agggcttccg gcgcgttgcc ttggcgcatc agcgtgatgg tgcgggtcag ggcattggtg
     5461 atgcaagaaa aggccgatac gaccgtcagc gagcccggcc cggcatttcg cacgatcttt
     5521 tcgacctgcc ggatggcatc ggccgtgccg accgaggtgc cgccgaattt aatgaccttc
     5581 atgaaacacg ctccctcatc ggaagttcac aaaaccgaaa gaggtctgag tgggtaaaag
     5641 aggccggaaa agcggtcgcg ctcttcagaa gctctccacg atcactcgtg acagccgcgg
     5701 ggattcgacc ccgtcgtcca acgcccgcac cggcggcccg ccgcgtcgtt tcggcgccga
     5761 tgcccctgct ccggcatcat cggggaccgc ccctccgccg gagtgcctgc tcggcgctcc
     5821 tcttccagaa cgtgggtacc ataacaggga agatagcgct gtcaatgatt ttttttctga
     5881 tgtcgggccg attgacattt gccgcttttc cctatataac aagttgtcgt ttcaagaaaa
     5941 aaaaaggagg aatatcatga aagggaccgg tgtgaccgct cttctgttct tgtgcctgtt
     6001 gcttgccgtt tcgtgcggcg atgatggcac gaagaccatt cctgacaacg ctcaggtgcc
     6061 cgacgagagc gccgtgaccg acgacaccgt gaccgatgac accgtcgtcg acgacacggt
     6121 caccgacgat accgtcaccg atgataccgt cgctgacgac accatcaccg atgataccgt
     6181 catcgatgac accgttaccg acgacaccgt ggtcgacaac gaaacgcccg acga
//
LOCUS       DAOQUE010000115         6179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_14048_length_6179_cov_7.465382, whole genome shotgun sequence.
ACCESSION   DAOQUE010000115 DAOQUE010000000
VERSION     DAOQUE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14048_length_6179_cov_7.465382"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1001)
                     /locus_tag="PKH10_04840"
     CDS             complement(234..1001)
                     /locus_tag="PKH10_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNT27491.1"
                     /translation="MTNHRIIGFCTLNRVGRTDTKQKEALTAYAAEKTLILEIVEGRY
                     SHKKKWKARTELVKLLEGLKPGDTLVTPTLSLLGLDSGDILSFIAELMKKKVDLHIVD
                     LGLVIAASGYSGDMIALFTLMTALTVPAKGKPGRKPGRPAKKKRGRPAKKKRGRPAKK
                     KPGRPPKKKKVGRPPQKKVGRPKTKLKKRGRKPARKKRVSSIQQQFNDKAFTIRKYLK
                     SQFPVQRIAEKLGLPYQPLLALLKKHPKLKGLVKKGR"
     gene            complement(1013..1291)
                     /locus_tag="PKH10_04845"
     CDS             complement(1013..1291)
                     /locus_tag="PKH10_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017865561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT27492.1"
                     /translation="MLKSDLIRRMEEKAQLSSRDADKFVNLLLDSLTSALLREERIEI
                     RGFGSFMTRHYGAYKGRNPRDGSSIKIGAKRLPHFKAGRDLTERVNNK"
     gene            1480..1773
                     /locus_tag="PKH10_04850"
     CDS             1480..1773
                     /locus_tag="PKH10_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNT27493.1"
                     /translation="MSLTKIELAELTYENMKVDKKQAAALVEMFFEEMKAGLLRDGEF
                     QISGFGKLEVKQKRSRKGRNPKTGEDLILEARKVVGFKHSLVLKGRLNSGKSA"
     gene            1792..2325
                     /locus_tag="PKH10_04855"
     CDS             1792..2325
                     /locus_tag="PKH10_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27494.1"
                     /translation="MWQKLISEKLKLLDAFLRSNSWNGRENEVVNLFAHHILIGSKMT
                     NELAPSQIGIEVAVPQVTGSRKKLVRKDLVIWPEPLMTVWTPGAIPAVIMEWKRDRWS
                     DTKDDIAWLKTFTKVYPGVLGYAVCAFIGRGRGVRYAVISNGRIIEETEFRTTSQDAL
                     GGANGDRSRGRPSEEVE"
     gene            complement(2406..2609)
                     /locus_tag="PKH10_04860"
     CDS             complement(2406..2609)
                     /locus_tag="PKH10_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27495.1"
                     /translation="MIPREVAPGVIILISEEDAWREFLFGIPAPNPLSSKSGHIVVKP
                     GDNALSLVPDLAVPKEAFCQEKH"
     gene            complement(2606..3307)
                     /locus_tag="PKH10_04865"
     CDS             complement(2606..3307)
                     /locus_tag="PKH10_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27496.1"
                     /translation="MSLVVAIQVESNDVILAADRRGTIGDPRSLVALDEGVSKLQKYG
                     TQAVIGIVGTPAAVITPIRLAQETIAANPAAHPLTVLTGTLKAHYHEHFGLRPYITEK
                     PLVDARPVAIVLYADRRPHGQGLFQLPSPNNFTAMSDDRPYLMAGVSQYALYLFQRLW
                     KQNFTIEEAKYLAAFLICETSKLDPKVGPTPDILSLTEAGIQRSSEDEIAQIMVENTR
                     RFDSFAESFRGRRPI"
     gene            3475..4602
                     /locus_tag="PKH10_04870"
     CDS             3475..4602
                     /locus_tag="PKH10_04870"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HNT27497.1"
                     /translation="MFRVLYQLLALELLLLRLLFRRRNALLFENLALRQQLAMFHVKM
                     KPARADDTTRSFFVALSRVWDDWVSVLVTVKPETVIDWQRRRFRSYWRAISAHAAGSG
                     KPRAFEIRRQTRKIYAENGWGASRIYSELLMLGYTERELSESTVARCLRAIRKKTGPT
                     ADQTQSWKTFLKNHRDGIAAMDFFTVPTVKFQLLYVFFVISHARRKIIHFACTLNPTA
                     EWVMQHLREAFPFDEAPRYLIFDRGSPFPSLRASIQAMGISPIMTGYQSPWQNGVAER
                     FVLNVRSDLLNRVIILNEKHLHRLMKEYVAYYNDDRCHLAVSRDSPAGREKETRTKKG
                     GTHRLAATRRFAAPLHLEESGITAIFQRFAAYPLTGKLHRK"
     gene            complement(4817..5629)
                     /locus_tag="PKH10_04875"
     CDS             complement(4817..5629)
                     /locus_tag="PKH10_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT27498.1"
                     /translation="MRKILFILALLGVTCVINAKEITIEAAITKVKGIAENKYCFSEE
                     SSGVKISMSYWDKLIPASCRANGFGKCKIMRDSMYYELRKELGKNPFWHVVIGIDGTP
                     LEFFVGVKDGKLVEGPNNPSHFSFCDSLKNSNFLQKLFFYAINGELRGVKKIAPNIST
                     KEINTVKENKTPLSLATESGHYDLVKFLVEEGADVNLKCPIVRAISSWHNDTLDYLLS
                     KGANINCTDNDDNIYPLDIARMNCNKYAIELLEKKSAKEKGDWKGKNDLCNF"
     gene            complement(5637..>6179)
                     /locus_tag="PKH10_04880"
     CDS             complement(5637..>6179)
                     /locus_tag="PKH10_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNT27499.1"
                     /translation="RWYDPETGRWLEKEPLGFNGSSNFYVYANNDPVNYVDRTGLRPV
                     EDRYDDYETALLNAYIDLLWAINFEYGACYQSNANTGERGTREWGVEIYFDKNSGKYL
                     YGVPYTTNPPYPEDKIPVTDSLPWFTLAGFAHTHPFSNDPSFSEDDINNANHRKPFPI
                     NFHTITQDPNGNWNITDYIR"
BASE COUNT         1433 a         1507 c         1640 g         1599 t
ORIGIN      
        1 ccgacccgac gaacggttaa ttgctgttgt tcatctttct cgcaaagccg atagttcccg
       61 gcggcaggcc gattgaccac gagagcaacg atactccgaa agaaaaagaa aggaccttgc
      121 atgagaggta ctccttaaga atatccgtat atcggccgac aagagaaaca ccatactcta
      181 ttgacaatga aaatatactc acatgaaaaa gaaagcaaga aaacgggaaa ggatcagcgc
      241 cctttcttca ccagcccctt gagtttcgga tgcttcttga ggagcgcgag taacggctgg
      301 taggggagac cgagtttctc ggcgatcctt tggaccggga actggctctt gagatacttg
      361 cggatggtga acgccttatc gttgaactgc tgttggatgg aacttacccg tttcttacgc
      421 gccggcttgc gtccccgttt tttcaacttg gtcttcggac gaccgacctt cttctggggt
      481 ggacgcccta cctttttctt cttgggcggc ctgcccggtt tcttcttcgc gggacgaccc
      541 ctctttttct tggccggacg accgcgtttc ttcttcgccg gtctgcccgg tttccggccg
      601 ggtttcccct tggccggtac ggtaagggcg gtcatgaggg tgaagagggc gatcatatct
      661 cccgagtaac ctgaagcggc gatgacgagg ccgaggtcaa caatgtggag gtcgactttc
      721 ttcttcatga gttcggcgat gaaagaaagg atgtcgccgg agtcgaggcc gaggagcgat
      781 agggtggggg tcactagggt gtcgccgggt ttcagccctt cgaggagttt cacgagttcg
      841 gtgcgggctt tccatttctt cttatgggaa tagcgcccct caacgatctc aaggatcaag
      901 gttttttccg ccgcataggc ggtaagggct tctttctgtt tcgtgtcggt tcttccgacg
      961 cggtttaagg tacagaagcc gataatgcga tggttggtca tgtgttttct ctctatttgt
     1021 tgttcacacg ctcggtgagg tcgcgccctg ctttgaagtg ggggagccgt ttggcgccga
     1081 tctttatcga ggaaccgtcc cgcggatttc tgcctttata ggcgccgtaa tgccgtgtca
     1141 tgaaactgcc gaagcctcgt atttctatcc gttcctcccg tagaagcgcg gaggtcagtg
     1201 agtctaacag gaggttcacg aacttgtcgg catcacggga actgagttgc gctttttctt
     1261 ccatgcgtct gataaggtcc gatttgagca tgcggggtct ccctaggtta gaaggatatt
     1321 ttattgatta tggggaggag tcaagaaatc atgggggaga ggggagggga ggggaaaccg
     1381 gaatcgttcc cggctgcgcc ggtttttttg acttgaatag ggtgggggaa tactatgccc
     1441 tgtctgtggt ggataacatc gacgcgaagg ggagatgtca tgagtctgac gaaaattgag
     1501 ttggcggagt tgacctacga gaacatgaag gtcgataaga agcaggcggc cgcgttggtc
     1561 gagatgttct ttgaggagat gaaggcgggg ctcctgcggg acggggaatt ccagatatcg
     1621 ggcttcggta aacttgaggt caaacagaag cggtcgcgta agggccgtaa tcctaagacg
     1681 ggcgaagacc tgatcctcga ggcgcggaag gtcgtgggct ttaagcatag tctcgtgctc
     1741 aagggaaggt tgaatagcgg gaaatccgcc taaataagcg gcgataagat gatgtggcag
     1801 aaactgatct ccgaaaaact caaattgctt gatgcgttct tgaggtcgaa ctcatggaac
     1861 gggcgagaga acgaggtagt caatctgttc gcacatcaca tcttgattgg ctcaaagatg
     1921 accaatgaac tggcaccgtc gcaaattggg atcgaggtgg ccgttccgca ggtgaccggt
     1981 tctcgaaaga aactggtccg aaaggatctg gtgatatggc cggagcctct gatgactgtt
     2041 tggacgcccg gagcgatacc agcggtgatc atggagtgga agcgtgaccg gtggagcgac
     2101 acaaaggatg acatcgcgtg gctgaagact ttcaccaagg tctatcccgg tgtcttggga
     2161 tatgcggtct gcgccttcat cgggcgcggg cgcggggttc gctatgcggt gatatcgaac
     2221 gggcgcatca tcgaagaaac agagtttcga acgacctccc aagatgccct aggcggggcg
     2281 aacggggacc ggtcaagggg tagaccttcg gaagaagtcg aataatatca aatagttacc
     2341 ttcgcataat atccattatg tcaacttgaa ggtgtttttc aagggtcccg aagctttgac
     2401 agaggtcagt gcttttcctg acagaaggct tctttgggga cggccagatc gggaacgaga
     2461 gacaaagcat tgtcgccggg cttaacgacg atatgccccg attttgacga aagcggattt
     2521 ggagcgggga taccgaacag aaactcgcgc catgcgtcct cctcgcttat gagaatgatc
     2581 acgcctggcg ccacctcgcg aggaatcata taggcctcct tcctctgaag gattctgcaa
     2641 agctatcgaa ccgtctagta ttctccacca tgatctgtgc tatttcgtct tcggaagatc
     2701 tttgtatgcc tgcttcggta agagataaga tatccggggt cggacccacc ttcggatcaa
     2761 gtttgctggt ttcgcagatc aagaacgctg ccagatattt cgcttcctcg atggtaaaat
     2821 tctgtttcca gagccgctgg aacaggtaga gcgcatattg ggacaccccg gccatgagat
     2881 aaggtctgtc gtcgctcatc gcggtgaaat tattcggcga tgggagttgg aacaatcctt
     2941 gcccatgcgg cctgcggtcg gcatagagga ctattgcgac aggccgagca tcgacaagcg
     3001 gtttctcggt tatataaggc ctgaggccaa aatgctcatg ataatgtgct ttaagagtcc
     3061 cggttaatac cgtaagcgga tgagctgccg ggttggcagc aatagtttct tgagcaaggc
     3121 gaatgggcgt aatgaccgct gccggcgtac caactatacc gatcaccgct tgcgtaccat
     3181 atttctgtaa tttcgatacg ccttcatcca atgcgacaag acttcgcgga tcgccaatcg
     3241 tacctctgcg gtcggcggcc aaaatcacat cattgctttc gacttgtatt gctactacca
     3301 aagacattga ggattctccc tttaatctcc tccaagagta tagtattcac tccggaacgg
     3361 tttgtcaaga atcctaccct aaacggaccc ccagtgaatc ctttcattcc ggcacctatt
     3421 gtctataatt cgctcaccaa tgcaagattt ttactacccg agaaagaggt tctcatgttt
     3481 cgcgtgctct atcaattgtt ggcgctcgaa ctgcttctgc tccgcttgct ctttcggcgg
     3541 aggaacgctc ttctgttcga gaatctggcg ctccggcagc agttggcgat gttccatgtc
     3601 aagatgaagc cggcgcgggc cgacgataca acccggtcgt tcttcgtggc gctcagccgg
     3661 gtatgggatg actgggtttc ggtgctggtg acggtcaagc ccgaaacggt gatcgactgg
     3721 caacggcgac ggttcaggag ttactggcgc gccatctccg ctcatgcggc aggtagcgga
     3781 aagccgcgcg ccttcgagat caggcggcag acacggaaga tctatgcgga gaacggttgg
     3841 ggcgcttccc gcatttattc ggagttgctg atgctcggct ataccgaacg ggagttgtcg
     3901 gaatcgacgg tcgcccggtg cctacgggcg atacggaaga agaccggtcc gaccgccgat
     3961 cagacgcagt catggaagac ctttctcaag aatcaccgtg acggtattgc ggcgatggac
     4021 ttcttcacgg tgcccacggt gaagttccaa ctcctgtatg tgttcttcgt catctcccat
     4081 gcgcggcgga agatcatcca tttcgcctgt acactcaatc ccacggccga atgggtcatg
     4141 caacatctcc gggaggcgtt cccgttcgat gaggcgccgc ggtatctcat cttcgatcgg
     4201 ggcagtccat tcccctcgct cagggcatca atccaagcga tggggatatc ccctatcatg
     4261 acaggttatc agtcgccgtg gcagaacggt gtcgcggaac ggttcgtgtt gaatgtccgt
     4321 tcggacttgc tgaatcgggt gatcatcctg aacgagaagc atctgcatcg gctgatgaaa
     4381 gagtatgtcg cctactacaa cgatgatcgg tgtcatctgg ccgtttcacg cgattcgcct
     4441 gcggggcgcg agaaagaaac acggacgaag aaaggcggaa ctcatcgcct tgccgcgact
     4501 cggcggtttg cagcaccgct acacttggaa gaaagcggca taacggctat ttttcagcgt
     4561 tttgccgcgt atccgttgac gggaaaactg catcgaaaat gacttagata cggccttttc
     4621 tcgggacgca tcattgtttt tccacaagac acctcctctc tttcgatcat catgagaaaa
     4681 atggtcggca acacggggtt ctgaccatag atcagcacgc ttttcagggt tcccgaggat
     4741 ttcgaacctt tcgatcaact atcgaaaatc actgtccgat gttgatttga cagaggacag
     4801 gttgaacttg agcaaatcag aaattacaaa gatcattctt tcctttccaa tccccctttt
     4861 cttttgcaga ttttttttcc agtagctcaa tcgcgtattt gttgcagttc attctggcaa
     4921 tatcgagcgg gtaaatgtta tcatcattat ctgtgcagtt gatatttgct cctttcgaga
     4981 gcagataatc cagtgtatcg ttgtgccaag aagagatggc ccttactatt ggacatttca
     5041 aattcacatc ggcaccttct tctacgagaa acttcacaag gtcataatgg ccgctctcag
     5101 tggcaagaga caatggggtc ttattctctt tgaccgtatt tatttctttc gttgagatgt
     5161 taggcgcgat cttctttaca cctcttaact ctccgttgat cgcataaaag aagagtttct
     5221 gtaagaaatt gctatttttc aaagaatcgc aaaacgaaaa atgtgatgga ttgttagggc
     5281 cctcaacaag ctttccatct ttgacaccaa caaagaactc caaaggagta ccatcaatac
     5341 cgataacaac atgccagaac ggatttttac cgagttcttt tctgagttcg taatacatag
     5401 aatcccgcat gattttacac ttcccgaaac cattcgctct acatgatgcc gggataagtt
     5461 tgtcccagta tgacatggat atctttaccc ccgaagactc ctcggaaaaa caatatttgt
     5521 tttcagctat ccctttgacc ttggtgatag ccgcctcaat cgttatttct tttgcgttta
     5581 tcacacaggt aacgcccaga agcgccaata taaataggat ttttctcatg acaacctcat
     5641 cgtatgtagt ctgttatatt ccaattcccg tttggatctt gtgttatcgt atggaaatta
     5701 atcgggaacg gctttcgatg gtttgcatta tttatatcat cttccgaaaa actcggatcg
     5761 ttgctaaatg ggtgtgtatg agcaaaacct gccagagtaa accacggaag actatccgtt
     5821 actggtatct tgtcttccgg atatggcgga ttggtagtgt agggaactcc gtataagtat
     5881 ttcccgctgt ttttgtcaaa gtagatttca acaccccatt ctcttgttcc tcgttctccg
     5941 gtgttggcgt ttgattgata acatgcccca tattcaaagt tgattgccca aagcaaatca
     6001 atataagcat ttagtaacgc cgtctcatag tcatcgtacc tgtcttcaac tggtcgcaat
     6061 cccgtcctgt ccacataatt caccggatca ttattggcat acacatagaa gttcgacgac
     6121 ccattgaacc ccagcggttc cttctccagc caccgccccg tctcggggtc ataccaccg
//
LOCUS       DAOQUE010000116         6169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_14083_length_6169_cov_3.997710, whole genome shotgun sequence.
ACCESSION   DAOQUE010000116 DAOQUE010000000
VERSION     DAOQUE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14083_length_6169_cov_3.997710"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="PKH10_04885"
     CDS             <1..1590
                     /locus_tag="PKH10_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT27500.1"
                     /translation="ALGRETARDLTALGFDVCEGYGMTETAPIITFPRLWKARIGAAG
                     QPLFPDSVRIEEGEVLVRGPHVMRGYWNRPEETAAIIRDGWLHTGDAGYLDHDGYLFI
                     TGRVKEIIVLSNGKNVNPEEVEDKLRRDFPAVAEAGVYLKEGALAAIIVPDPARVPPA
                     AKRDLRDYFRYEVIQRYNEKATPYKRLLHFTLSSDPLPRTRLGKLRRFQLPALGEEGK
                     RRRAAADAPDTAEYAAITRFISDETGKEPHPDDHIEIDLGLDSLSRLKLQEFIRASCG
                     VALRDGEIAGTVRTLAERVGAARGTVIEQSVGWKELLLRDEDIDLPRSAWFHAFLKNM
                     FRLWLYTYFRFRANGRENVPEPPFILAANHQSFIDGLFVVTPLKDDLVRETFFFAKGK
                     HFKAWWRRYLARRDNIIVMDEQRDLRDAISRMAAVLRKKKSIIIFPEGTRTRDGHIGN
                     FRETFAILAAELKVPVVPVAIRGAFEAFPRGRFIPRPWRSISVTYLPPVSPDGRSYQA
                     LATEVRDAIAAELGKTIAEVR"
     gene            complement(1634..2812)
                     /locus_tag="PKH10_04890"
     CDS             complement(1634..2812)
                     /locus_tag="PKH10_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27501.1"
                     /translation="MSIRLFTAMTAGLLAAFYSGCGGRSSGTDPSRDEDVIPSPDEYG
                     SGMACVDATVSYEKGINSPDSMGSTIIIPKGTKGRVVGQFNFAARNCAKNDFFYFVSL
                     QLKIQRDDPNIILKNIEMVYDENRNGAVDPDEPVVAHKEVIDSTGVLLVLDPQKSSFA
                     GSEEHYFVIRADVDFAIARVPGGASFRFIIESEESFSFSGAGVAKPDGAAINFADFLV
                     EPTENYFIVTRGDNDPVPPKIPVRTEVNKDIPVLQLRTKALDAANRIRRIRIGIPSDD
                     YVTFGTGIRSVSIYLDLGGDGILDPMIEEERVPIEKIDEFDDEFDVTFESEGLANKLH
                     YSEGQEIFLVVKAHFGLMSNDKAKIELNRGAVLLEDTSKKVEGLPVFSKEFYIVDLEE
                     "
     gene            complement(2921..3946)
                     /locus_tag="PKH10_04895"
     CDS             complement(2921..3946)
                     /locus_tag="PKH10_04895"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNT27502.1"
                     /translation="MDAFIRKTRDERRLIFEQVESRRKLPAYSIEKDLWLSLAVRELF
                     SMTAIGPHLTFKGGTALSKVWKIIDRFSEDVDLTIDRAALGFSGDTAPETAPSMKKRR
                     QRLDELKESCRRYVHGPLAEGLRQRLAEKLPSGESWDLVGAEDDPDGQTLLFQYPSCW
                     DTALARYVTPAVRLEFGARSDPWPASDRSITPMVAEEFPQLFTVMSCSVRALAAERTF
                     WEKAMLLHEENSRPLEKGHKRRMARHYYDLWALIRHGVDRSAVADEALFEAVAAHRRI
                     FFNWSWMDYDTLRKGSLRIVPLPDRMSAWQRDYDEMRREMFFDEPPTFADVIATVRAF
                     ENRSNHG"
     gene            complement(3957..4568)
                     /locus_tag="PKH10_04900"
     CDS             complement(3957..4568)
                     /locus_tag="PKH10_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HNT27503.1"
                     /translation="MVHAMQGLDTKILNRVRGKGKGSLVMPGDLLDLGTRRAIDVALH
                     RLVKKGVLRRLARGIYDLPRTHPELGLLMPSADEVAKALAGRDRTRLQPSGAYAANLL
                     GLSTQVPMKIVFLTDGPSKRVRIGKAEIILKRTTPKNMATAGTISGLIIQALRYLGRN
                     AVDETIIAQLRSKLDTAGKNRLSRDAALAPAWIGVIMRQLAAE"
     gene            4751..>6169
                     /gene="dnaE"
                     /locus_tag="PKH10_04905"
     CDS             4751..>6169
                     /gene="dnaE"
                     /locus_tag="PKH10_04905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNT27504.1"
                     /translation="MPFVHLHVHTVYSFLDGLTRPSALMKEAARLGMPAIAITDHGNM
                     HGVIDFYLSAFREVEDPATKKKKKERFIRPLIGMEAYIAGGSRKERVSQQDAFHMVLL
                     ARNREGYRNLMYLSSQSYLDGFYYKPRIDRELLKEHAGGLIATSACLAGEIPRALKDG
                     NKKRALQIASEYRELFGADNFFLELQVNGIKEQDEVNPLIMDIGRELDIPFVATNDVH
                     YLRSEHAEAQDILFCINEKKKVEDADRMHHESREFYLKSEEEMRRLFAPLCAEAVDNT
                     GFVAGRCELELELDKSYLPHYDTGGVPLTDYLIDGARRGLDRHIVEGRIPTAKRELYE
                     QRLAHESDIISAKGYQGYFLIVADFIGWAKRNGVPVGPGRGSGAGSLIAYCLDITEID
                     PIRYGLFFERFLNPERPSMPDFDVDFCQQKRERVIEYVTQKYGAENVAQIATFSKMKA
                     KAAIRDVARVLAIDLPTADRLAK"
BASE COUNT         1271 a         1944 c         1765 g         1189 t
ORIGIN      
        1 gcgctcggcc gcgaaacggc gcgcgatctc acggcgctcg gcttcgatgt ctgcgaaggc
       61 tacggcatga ccgaaacggc ccccatcatc accttcccgc ggctctggaa ggcgcgcatc
      121 ggcgcggcgg gccagccgct cttccccgac agcgtgcgca tcgaggaagg tgaagtgctg
      181 gtgcgcggtc cccatgtcat gcgcggctac tggaaccgcc ccgaagagac cgccgcgatc
      241 atccgcgacg gctggctcca taccggcgac gcggggtatc tcgaccacga cgggtatctc
      301 ttcatcaccg gccgcgtcaa ggagatcatc gtcctttcca atggcaagaa tgtcaatccc
      361 gaggaggtgg aggataaact gcgccgcgac ttcccggcgg tggccgaagc gggcgtctac
      421 ctgaaggagg gcgcgctcgc cgccatcatc gtccccgatc cggcgcgggt gccgcccgcc
      481 gcaaaacgcg acctgcgcga ctatttccgt tatgaagtga tacagcgcta caacgaaaag
      541 gcgaccccct acaagcgttt acttcacttc acgctgtcga gcgacccgct cccccggaca
      601 cggctgggga aactgcgccg cttccagttg ccggcgctcg gcgaggaggg gaaacggcgc
      661 cgcgcggcgg ccgacgcccc cgacacggcc gaatacgccg cgataacgcg cttcatctcc
      721 gacgaaacgg ggaaagagcc ccatcccgac gaccacatcg agatagacct cggactcgat
      781 tcgctctcgc ggctcaaact acaggaattc atccgcgcct cgtgtggcgt cgccctgcgc
      841 gacggcgaga tcgcggggac ggtgcggacc cttgccgagc gggtgggagc ggcacgcgga
      901 acggtgatcg aacagtcggt cggctggaag gaactcctcc tccgggacga ggatatcgac
      961 ctgccgcgca gtgcgtggtt ccatgctttc cttaaaaaca tgttccgcct gtggctctac
     1021 acctatttcc ggttccgcgc caacgggcgc gagaatgtgc ccgagccgcc cttcatactc
     1081 gcggcgaacc accagtcgtt catcgacggc ctgttcgtcg tgacaccgct taaagacgac
     1141 ctcgtgcgcg agaccttctt cttcgcgaag ggaaagcact tcaaggcgtg gtggcggcgc
     1201 tatctggcgc ggcgcgacaa catcatcgtg atggacgaac agcgcgatct gcgcgatgcg
     1261 atcagccgga tggcggcggt actgaggaag aaaaagtcga tcatcatctt ccccgaaggg
     1321 acgcggaccc gtgacggtca tatcggtaac ttcagagaaa cattcgcgat actggcggcc
     1381 gaactcaagg tgccggtggt gccggtggcc atccgcggcg cgttcgaggc gttcccgcgc
     1441 ggccggttca taccgcggcc gtggcgttcc atatcggtga cctacctgcc gccggtgagt
     1501 cccgacggcc gctcctatca ggcgcttgcc accgaagtgc gcgacgccat cgccgccgaa
     1561 ctcggcaaga ccatcgccga ggtccggtaa aagaattctc gtctactatc tttttgtact
     1621 tccgggaacc gagttattct tccagatcta caatataaaa ttcttttgag aaaaccggca
     1681 acccttcgac cttttttgat gtgtcctcca gcaacacggc cccccgattg agctcgatct
     1741 tcgccttgtc gttgctcatc aagccgaaat gcgccttgac gacgaggaat atctcctgcc
     1801 cttcgctgta gtgtaattta ttcgccaagc cctcgctttc gaaggtgaca tcgaactcgt
     1861 cgtcgaactc gtcgatcttt tcaatgggta cccgctcctc ctcgatcatg gggtcgagaa
     1921 tgccgtcgcc ccccagatcg agatagatcg ataccgaccg tatcccagtg ccgaaggtga
     1981 catagtcgtc ggaggggatg ccgatcctga tgcggcggat acggttggcg gcatcgagcg
     2041 ccttggtacg cagttgcagg acggggatat ctttattcac ctccgtacga acggggatct
     2101 tcggcggcac gggatcgttg tctccccgag taacgatgaa ataattctcg gtcggctcca
     2161 cgaggaaatc ggcgaagtta atcgccgccc cgtccggttt tgccacaccg gcacctgaaa
     2221 aggagaatga ctcctcgctt tcgataatga accggaacga agcgccgccc ggcaccctag
     2281 cgatcgcgaa atcgacatcg gcacggatga cgaaatagtg ctcctcgcta ccggcaaagg
     2341 aggacttttg cggatcgagg accagaagga cgccggtgga gtcgatgacc tctttgtggg
     2401 cgacaaccgg ctcgtcagga tcgaccgctc cgttcctgtt ctcgtcgtac accatctcga
     2461 tgttcttaag aatgatattg ggatcgtccc gctgtatctt caactgcagg gaaacgaagt
     2521 aaaagaaatc gttcttcgcg cagtttctcg ccgcgaaatt gaactgaccg acgaccctcc
     2581 ccttcgtccc tttcgggatg atgatcgtac ttcccatgct gtcgggcgag ttgatccctt
     2641 tttcgtagga aaccgttgcg tcgacgcatg ccatcccact gccgtactca tccggtgacg
     2701 gtataacatc ttcgtcgcgt gagggatcgg tcccggaaga tcgtccgcca cacccggagt
     2761 aaaacgccgc gagcagtccc gccgtcatgg ccgtaaaaag acgaatggac ataagccctt
     2821 cccctagaag taccgccgcc attttaaacc cttagtgaaa taaatgcaat agcccccgcc
     2881 ggaagatttt tcccaatgat tccgttcggg ggaatggttc tcaaccgtgg ttggatcggt
     2941 tctcgaacgc ccgcaccgta gcgatgacat ccgcgaaggt cggcggctca tcgaagaaca
     3001 tctcccggcg catctcgtca taatcgcgtt gccatgcaga catccggtcg ggcaacggca
     3061 cgatcctgag tgaacctttg cgcagcgtat cgtaatccat ccatgaccag ttgaaaaata
     3121 tccggcgatg cgccgcgacg gcttcgaaaa gcgcttcatc ggcgacggcg cttcgatcca
     3181 cgccatgccg tatgagcgcc caaagatcgt aataatggcg cgccatgcgg cgcttatgcc
     3241 ctttctccag cggccgggaa ttctcttcgt gcaacagcat cgccttttcc cagaaggtcc
     3301 gttcagccgc caaagcgcgc accgaacatg acatcacggt aaaaagctgc gggaactcct
     3361 ccgcgaccat cggcgtgatg gaacggtcgc ttgctggcca cggatcggag cgtgcgccga
     3421 attcaagccg cacggcgggc gtcacatagc gggcaagagc cgtatcccag caggagggat
     3481 actggaacag gagcgtctga ccgtcgggat catcctccgc accgacgaga tcccacgact
     3541 caccggacgg cagtttctcc gccaagcgtt gccgcagtcc ctccgcaagc ggaccgtgaa
     3601 cataccggcg gcacgactcc ttgagttcat cgagccgctg tcggcgtttt ttcatgcttg
     3661 gcgccgtttc gggagcggta tcgccgctga acccgagtgc cgcacgatct atcgtcaggt
     3721 cgacatcctc ggaaaagcga tctatgatct tccacacctt cgacagcgcc gtgccacctt
     3781 tgaaagtaag gtgtggaccg atggccgtca tcgagaacaa ttcgcgcacc gcgagcgaaa
     3841 gccagagatc tttttcaatg ctgtaggccg gaagtttccg ccgcgactcg acctgctcga
     3901 atatcaggcg gcgttcgtcg cgcgttttac gaatgaaagc gtccatcgtt tacccgttat
     3961 tccgccgcca gttgtcgcat gatgacgccg atccatgccg gggcgagcgc cgcatcgcgc
     4021 gacaaccggt ttttccccgc cgtatcaagt ttgctccgca actgagcgat gatcgtctca
     4081 tccacggcat tgcgccccag atagcgaagc gcttgaatga taagcccgct gatcgttccc
     4141 gccgtggcca tgttcttggg cgtggtccgt ttcaggatta tctcggcctt gccgatgcgg
     4201 acgcgcttgg aggggccgtc ggtcaggaat acgatcttca tcggtacctg tgtcgagagg
     4261 ccgagtaggt tggcggcata cgcgccggat ggttggagtc gtgtccgatc gcggcccgcg
     4321 agcgccttcg ccacctcgtc ggcagagggc atgagcagcc ccagttccgg gtgtgttcgc
     4381 gggaggtcgt agataccgcg cgcaaggcgg cgaagcaccc ctttcttgac cagccggtgg
     4441 agagccacat cgatggcgcg gcgggtgccg aggtcgagga ggtcgccggg catgacaagg
     4501 ctaccctttc ctttcccgcg gactctattt agtattttag tatcaagacc ttgcatggca
     4561 tgcaccataa aaatctgtaa gaaatagtat atcgtttttc ttacaaatca agataaactt
     4621 ggaaaataaa tgcgcgagca cccgccggaa gttttttccc aatgattccg ttcgggggcg
     4681 ctccggcccc tttacttgcc cggcgcgttc tatacaatag cccccgtccg acaaccgacc
     4741 ggggagtccc atgccttttg tccatctcca tgtgcatacc gtctacagtt ttctcgacgg
     4801 tctcacgaga ccttccgcgc tcatgaagga ggcggcgcgc ctcggcatgc ccgccatcgc
     4861 catcaccgac cacggcaaca tgcacggcgt gatagacttc tatctctcgg cgttccgcga
     4921 ggtcgaggat ccggcgacca agaaaaagaa aaaggagcgc ttcatacgcc ccctcatcgg
     4981 catggaggcc tacatcgccg gcggatcgcg caaggaacgg gtctcccagc aggacgcgtt
     5041 ccacatggtc ctgctcgccc gaaaccgcga ggggtaccgc aacctcatgt acctctcgtc
     5101 gcaatcctac ctcgacggat tctactataa accgcgcata gaccgcgaac tgctgaaaga
     5161 gcacgccggc gggctcatcg ccacctcggc ctgtctcgcc ggagagatac cgcgggcgct
     5221 caaggacggc aacaagaaac gggcgctcca gatagcgtcc gaataccgcg aactgttcgg
     5281 cgccgacaat ttcttccttg agttacaggt gaacggcatc aaggagcagg acgaggtcaa
     5341 cccgctcatc atggatatcg gccgggaact cgacataccc ttcgtagcga ccaacgatgt
     5401 gcactacctc cgctcggaac acgccgaggc gcaggacatc ctgttctgca tcaacgaaaa
     5461 gaaaaaggtc gaggacgccg accgcatgca ccacgaatca cgcgaattct acctgaagag
     5521 cgaggaggag atgcggcggc tgttcgcccc gctctgcgcc gaggcggtcg acaacaccgg
     5581 ctttgtcgcc ggacgctgcg aactggaact ggaactcgac aaaagttacc tcccccacta
     5641 cgacaccggc ggcgtcccgc tgaccgatta tctgatagac ggcgcgcggc gcgggctcga
     5701 ccggcatatc gtcgaggggc gcatcccgac cgcgaaacgg gaactgtacg aacagcggct
     5761 cgcccatgag agcgacatca tatcggccaa gggctatcag ggctacttcc tcatcgtcgc
     5821 cgacttcatc ggctgggcca agcgcaacgg cgtcccggtg ggtcccggcc gcggatcggg
     5881 ggccggctcg ctcatcgcct actgcctcga tatcaccgag atagacccga tacgctacgg
     5941 gcttttcttc gagcggttcc tcaatccgga gcgtccctcg atgcccgact tcgatgtcga
     6001 tttctgccag cagaaacggg aacgggtcat cgaatatgtc acgcagaagt acggcgccga
     6061 gaatgtcgcg cagatagcga ccttcagcaa gatgaaggcg aaggcggcca tccgcgatgt
     6121 cgcccgcgtc ctcgccatcg acctgccgac cgccgaccgg ctcgccaag
//
LOCUS       DAOQUE010000117         6141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_14168_length_6141_cov_4.976832, whole genome shotgun sequence.
ACCESSION   DAOQUE010000117 DAOQUE010000000
VERSION     DAOQUE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14168_length_6141_cov_4.976832"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PKH10_04910"
     CDS             complement(<1..1215)
                     /locus_tag="PKH10_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27505.1"
                     /translation="MRNIIVAIIAVLLLTTGHLYAQCDTHNCTAQISNASGATGDWST
                     VVVDLRAGQYVRFQVVAGVTYEWSTCGEFSPGFDTEITIKSSASGTYACSEKATAVAS
                     IDPATDVLTSTAHGLGNDTEGYFVATGFPTGLAPATSYYVVNRTADTFQVAVTPDGTP
                     IDISSAGTAVVFYSKTLSGTPVAGGYNDNDTGCAYGTMSTVSWKAPADGTVAVLVNES
                     TCTTNDLPFSSVDLKWRIACRECTGPALSGDPLQSSSSSLWSTVATDLEGGQHVSFDL
                     VAGTPYTWTTCLADPNDPDKPTFDTELSLFEGAVTCSGAPATLNGRILAYDDDDTACE
                     HGMSTLSWTPANDMEVAVLVTEKDATGICRTNGATDSNVSLRWRVNGCRNCTTTPDPA
                     VFSEDAIPAWTSS"
     gene            complement(1227..2021)
                     /locus_tag="PKH10_04915"
     CDS             complement(1227..2021)
                     /locus_tag="PKH10_04915"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT27506.1"
                     /translation="MRTLWFVCVLAVMSVVACGGKNEAVRDIEVVIDTDVVVLDEEPA
                     TDTDGGTDADIKPVAEGPSGEQQWFGVMPTGQSKCYDNEKEISCPEPGERFYGQDAQF
                     NQWKARSLLQNEDDGDVPPGTVFDDVTRLYWEQAYLSGVNWSQAQNYCSALSLAGKSW
                     RLPTPHELKSLIDYGSQTHPATDFPADTTKELGPCYADHTCDPGRACLEKDGKWRCYS
                     STPNDWFWSSMRVQDPLTAWVVYFHDGYLEYTARDNVYSVRCVSSL"
     gene            complement(2069..2485)
                     /locus_tag="PKH10_04920"
     CDS             complement(2069..2485)
                     /locus_tag="PKH10_04920"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HNT27507.1"
                     /translation="MERRGFTLMEIMVVMLIIAVLAAVAIPMYQDYTKKTKAAEAWEE
                     LSNIASLQEQIFTDLRQYDTDGSRLPAYGATFQGKYFSVEVNGTNVWTAAASVCFDGR
                     KPCSLTDGYDYMFTIDRSGYKTTKPNGANVYPGWKL"
     gene            complement(2500..2985)
                     /locus_tag="PKH10_04925"
     CDS             complement(2500..2985)
                     /locus_tag="PKH10_04925"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT27508.1"
                     /translation="MRTAVRGYTLVELLIVMAVVSILAATALVSYDEYARKSRVENLK
                     ARILDAAVAQERFFTARGRYTSVLSDLLDFGWKDPNPEGVRITTGVFLGVGVNQSFWV
                     AGHTDIDKTLDAYHECWFYPGRTPVTTARVANGFVQLWDDRKNQQTATVPYTTLDGSC
                     K"
     gene            complement(2990..3796)
                     /locus_tag="PKH10_04930"
     CDS             complement(2990..3796)
                     /locus_tag="PKH10_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27509.1"
                     /translation="MLRTIRHLLAAILLMAGLSANSIAAETTVNDSLALFPDLVIVGQ
                     VIAVDPFSVTIQNTRYTTTLTPQRLDALRARLTASKDDADSAFQKALRTIKVTDDSHM
                     QMVFYFDTVNQLFHDDINTYQQFLPLFYLFKSNIKELQGQSKDIIEKFSQMKADYFLT
                     IPFVKKLMKNAWFAAVFRIHPETKKIIYRHSRFYETYEELEEDAYARDNQIRKIVEKG
                     LTTTLTVAVVRGHESQWLKEDPHKKYRDGLKGGKGGKRYNTPAGTMGGGK"
     gene            complement(3808..>6141)
                     /locus_tag="PKH10_04935"
     CDS             complement(3808..>6141)
                     /locus_tag="PKH10_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27510.1"
                     /translation="WSRPTSGDECLLGKHDMTIPGDDDCEWLTFSSDDLLQDCFNFNA
                     PATATNGTDPGMTDYNKVLQGGVAANLAVQAGTCGTPPCYREHAACVWSSAAQCRIIY
                     TDNGAGPLVNFASIPPGDLVSRPAEPYIGYFDGISTANAVTILDYVYGYDSVDHNGDG
                     DLTQNRATELPALEKLSDPFVSGATIDLSYSILGAAVHSKPLAVYYSSSGATPTEADL
                     RVFYGANDGLLHCFDGVETAGHPNERWAYMPSIVRNEMPFILSGLDSVSFNSIIDGPM
                     TLFHVDSDVDGFIDYPGEAAFLIIAYRRGIGVNSAYTILDISDPDNPELVEHLSITGQ
                     SWSAPALFRMGGNGPYYLAFGGGYDPCVDDDSPSCGLPDNEADCVNDTQCDPGYKCDI
                     GLGKCRHQIKGNQIHIYEYNGNPSSPDFSHVKTFAHDPADDHLKWLVAPVAAEGVAVN
                     TEGPLFTKISGVYSGNVEMLYFVDVTGTVFRVYHNGTDWQLRTVMTMRAQPAPEFFNF
                     GQGVRSYYSFMPSPKFEQFTKMQIDHGGDGDMGTANDVFIVPVPLVTGNIVYPREAGV
                     PYEILVLYDRLPNPPGGTLPGGDVALTALYSSADFKEMTDRETTHDLPFRDGENTYDA
                     LYNGYRIDLRPNVFPGPPDPDTGVSASLEGEKNMAAPLVYYNWDEDTHALFATTYATQ
                     TGLNECSNSGESFLYCRNLPTGDTILLDDRYAKCAACNVIGLGKGLAQTPSVLKGIRQ
                     PNDEVVVVAVGADLKTESSELPPPPKTIKILKWYELY"
BASE COUNT         1234 a         1779 c         1816 g         1312 t
ORIGIN      
        1 cgaagaggtc catgccggta tggcgtcctc ggagaacacg gccggatcgg gcgtcgtcgt
       61 gcagttccgg cacccgttca cgcgccatcg gagagaaaca ttggaatcgg tcgcgccgtt
      121 cgtacgacag atcccggtcg catccttctc ggtgacgagt acggcgacct ccatgtcgtt
      181 tgcgggggtc caggaaaggg tggacatgcc gtgttcacag gcggtatcgt cgtcgtcata
      241 ggcgagtatc ctgccattca gggtggcggg cgcacccgaa caggtgaccg cgccctcgaa
      301 aagcgacagt tcggtatcga aagtgggttt gtccggatcg ttcggatcgg caagacaggt
      361 ggtccaggta tagggggtgc cggccaccag atcgaaactg acatgctgtc caccctccag
      421 atcggtggcc accgtggacc ataaggagga cgaactgctc tgtagcgggt caccgctcaa
      481 tgcgggaccg gtgcattcgc gacaagcgat acgccatttc agatcgacag aggagaacgg
      541 caggtcgttg gtcgtacagg tgctttcgtt caccaatacc gcaacggtgc cgtcggcagg
      601 cgctttccag gaaacggtgg acatcgtacc gtaggcgcat ccggtatcgt tatcgttgta
      661 ccctccggcg acgggcgtac cgctcagggt ctttgagtag aacaccacgg cggtcccggc
      721 cgaggagata tctatcggcg tgccgtccgg cgtcaccgcc acctgaaaag tgtcggcggt
      781 gcgattgacg acataatagc tggtcgccgg ggcaaggccg gtcgggaatc cggtggcgac
      841 gaaataacct tccgtgtcgt tcccgaggcc atgcgccgta ctggtgagca catcggtagc
      901 gggatcgatg ctcgccaccg cggtggcttt ttcagagcag gcataggtac ccgatgcgga
      961 agattttatc gtgatctcgg tatcgaatcc gggagaaaac tctccacagg tcgaccattc
     1021 ataggtaacg ccggctacga cctgaaagcg aacatactga ccggcccgca ggtcgacaac
     1081 gacggtggac cagtcaccgg tagcgcccga cgcattggat atctgtgccg tgcagttatg
     1141 ggtatcacac tgagcgtaca ggtgcccggt ggtcagaagc agcaccgcga taatggccac
     1201 gataatgttt ctcatgacac cctccctcaa agactcgata cacagcggac actgtacaca
     1261 ttgtcccgcg ccgtgtattc gagatatccg tcatgaaagt agaccaccca cgcggtaagc
     1321 ggatcctgca cgcgcatcga agaccagaac cagtcgttcg gggtcgagga gtagcatctc
     1381 catttgccgt ctttttccaa acaggcacgc cccgggtcgc aggtatgatc ggcataacag
     1441 gggccgagtt cctttgtggt atcggcggga aaatcggtcg ccggatgggt ctggctaccg
     1501 taatcgataa gacttttcag ttcgtgcggg gtcggtaggc gccacgattt cccggccaga
     1561 gaaagcgccg aacagtaatt ctgcgcttgt gaccaattaa cgccgctcaa gtatgcctgt
     1621 tcccagtaca gccgggtcac atcgtcgaac acggtgcccg gcggcacatc cccgtcgtct
     1681 tcattttgca ggagagatct cgctttccac tggttgaact gcgcatcctg cccgtagaag
     1741 cgttcgcccg gttcgggaca cgatatctcc ttttcgttgt catagcactt ggactggccg
     1801 gtcggcatca cgccgaacca ctgctgttcg ccgctcggcc cttccgcgac gggctttatg
     1861 tcggcatcgg tgccgccgtc ggtatcggtt gccggctcct cgtcaagaac cacgacatcg
     1921 gtgtcgataa cgacctcgat gtccctgacc gcttcgttct tgccgccgca tgcgaccacg
     1981 gacatgactg caaggacaca gacgaaccag agcgttctca tggcttcccc catcggatca
     2041 tttgttattt ttgcaagcgc ttttttcatc atagtttcca ccccggatag acattagccc
     2101 cgttcggttt ggtcgtcttg taccccgacc gatcgatggt gaacatataa tcgtacccgt
     2161 cggtgagact gcacggcttg cgaccgtcga agcacaccga cgcggcggcg gtccagacat
     2221 tggtgccgtt cacctcgacg ctgaaatatt tgccttggaa ggtcgccccg taggcgggga
     2281 gccgcgaccc gtcggtatcg tactggcgca ggtcggtgaa tatctgttcc tgcagggagg
     2341 cgatattgga cagttcctcc cacgcctcgg ccgctttggt ctttttcgta taatcctgat
     2401 acatcggtat cgcgacggcc gcgagcaccg cgatgatcag catcaccacc atgatctcca
     2461 tgagcgtaaa accgcgccgt tccatgctgt tatctcccgt catttgcatg atccatccag
     2521 ggtcgtgtag ggcaccgtgg ccgtctgttg gttcttgcgg tcgtcccaca actggacgaa
     2581 gccgttcgcc acgcgcgccg tcgtgaccgg cgtccggccg gggtaaaacc agcattcgtg
     2641 gtaggcgtcg agcgtcttgt cgatgtcggt gtgtcccgcc acccagaacg attggttgac
     2701 gccgacaccg aggaacactc cggtggtgat gcggacgcct tcggggttcg gatccttcca
     2761 cccgaaatcg agcaggtcgc tgagcacgga ggtgtagcga ccgcgcgcgg tgaagaaccg
     2821 ttcctgagcg accgccgcgt cgagtatccg cgccttgaga ttctcgacgc ggctcttgcg
     2881 cgcgtattcg tcgtaactca cgagcgcggt ggccgccaga atggaaacga ccgccatcac
     2941 gatgagcagt tcaacgagcg tgtagccgcg taccgccgtt ctcatgtcgt catttccctc
     3001 cacccatggt gcccgccggc gtattgtagc gcttaccgcc cttaccgccc ttgagcccgt
     3061 cgcgatattt tttatgggga tcctccttga gccactgcga ctcatgccca cgcacgaccg
     3121 ccacggtaag tgtcgtggtc agtccctttt caactatctt acggatctgg ttgtcccgcg
     3181 cgtaggcatc ctcctcaagt tcctcatagg tctcgtagaa ccgtgagtgt cgatatatga
     3241 tcttcttcgt ctcgggatgt atgcggaaca cggcggcgaa ccacgcgttc ttcatgagtt
     3301 tcttcacgaa ggggatggtg aggaagtaat ccgccttcat ctgggagaat ttctcgatga
     3361 tatccttgga ttgtccctgc agttccttga tgttgctctt gaacaggtag aacagcggca
     3421 ggaattgctg gtaggtgttg atgtcgtcgt ggaacagttg attgaccgta tcgaaataga
     3481 acaccatctg catgtggcta tcgtcggtca ccttgatggt gcgaagcgcc ttctggaagg
     3541 cgctgtcggc atcgtccttc gaagcggtca ggcgggcacg cagcgcatcg agccgctggg
     3601 gggtgagcgt cgtcgtgtat ctggtgttct gtatcgtcac tgaaaaggga tccacggcga
     3661 tcacctgccc cacgatgacg agatcgggaa atagagcgag agaatcattg acggtcgtct
     3721 ctgcggcgat cgaattcgcg gagagtcccg ccatcagcag tatcgccgca agaagatgtc
     3781 gtatcgtccg taacatatcc ttactcccta atataattcg taccacttga gtatctttat
     3841 cgtcttgggc ggcggaggca attccgagga ttcggtcttg agatcggcgc cgaccgcgac
     3901 caccacgacc tcgtcattcg gctggcggat acctttgagc accgacggcg tctgggcaag
     3961 cccctttccc aaccctatga cattgcacgc ggcgcatttc gcgtaccgat catcgagcag
     4021 gatggtgtcg ccggtcggca gattacggca gtacaggaac gattccccgc tattggagca
     4081 ctcgttaagg ccggtctgcg tggcataggt ggtggcgaac agcgcatgcg tgtcttcatc
     4141 ccagttgtaa tacacgagcg gtgccgccat attcttttct ccctctagac tcgccgagac
     4201 gccggtgtcg gggtccggcg ggccgggaaa cacattcggt cgcagatcga tgcggtaccc
     4261 gttgtaaaga gcatcatagg tgttctctcc atccctgaac ggcaggtcat gggtggtctc
     4321 ccgatcggtc atttctttga agtcggcgga cgaatagaga gccgtcagcg cgacatcgcc
     4381 ccccggaagc gtcccgcccg gcgggttggg caaccggtca tacagcacca atatctcata
     4441 gggtaccccc gcctcacggg gatagacgat gttgccggta acgagtggta ccggcacgat
     4501 gaacacatcg ttagcggtgc ccatgtcccc gtcgccgcca tggtctatct gcatctttgt
     4561 gaactgctcg aacttgggcg aaggcatgaa cgaatagtag gagcggacgc cctgaccgaa
     4621 attgaagaat tccggagccg gctgggcacg catcgtcatg accgtcctga gttgccaatc
     4681 ggtaccgtta tgatacacgc ggaagaccgt gccggtcaca tccacgaaat aaagcatttc
     4741 gacattgccg gaatagacgc cgcttatctt cgtaaaaagc gggccttcgg tgttcaccgc
     4801 gacgccttcg gccgccacgg gcgcgacgag ccatttcaaa tggtcgtcgg ccggatcgtg
     4861 ggcaaaggtc ttcacatgag agaaatcggg agaggacggg ttaccgttgt attcgtagat
     4921 gtgtatctgg ttccccttta tctggtgccg acacttaccg aggccgatgt cgcacttata
     4981 ccccgggtca cactgcgtat cgttcacgca atcggcctcg ttgtccggca gaccgcacga
     5041 ggggctatcg tcatcgacac aaggatcgta gccgccgccg aacgcgagat aatacgggcc
     5101 attaccgccc atacggaaca acgccggagc cgaccacgat tgtcccgtga tgctgaggtg
     5161 ttcgaccagt tccggattgt cggggtcgga tatgtcaagg atggtgtagg ccgaattgac
     5221 cccgatgccc cggcggtagg caatgatgag gaatgcggct tcaccggggt agtcgataaa
     5281 accgtccaca tcggaatcca catggaaaag cgtcatcgga ccatcgatga tcgagttgaa
     5341 ggaaacggag tcaagacctg aaaggatgaa cggcatctcg ttcctgacga tcgaaggcat
     5401 ataggcccac cgctcattgg gatgtcccgc cgtctctaca ccgtcgaaac agtgcagaag
     5461 cccgtcgttg gcgccataga acacccgcag atccgcctcg gtcggggtag cgccgctcga
     5521 agaataatag accgcaagcg gcttgctgtg gaccgccgcc ccgaggatgc tgtaagagag
     5581 gtcgatcgtc gctccgctga cgaagggatc gctcagtttc tccaaggccg ggagttcggt
     5641 ggcccggttc tgcgtgagat ctccgtcgcc attgtggtcg accgaatcgt agccatagac
     5701 ataatcgagg atggtaaccg cgttcgcggt gctgataccg tcgaaatacc ctatgtacgg
     5761 ttcagcggga cgggagacca gatccccggg cgggatagag gcgaaattca ccagaggccc
     5821 cgcaccgttg tcggtataga tgatgcggca ctgcgcggca ctcgaccaca cacaggccgc
     5881 atgctccctg tagcaggggg gcgttccgca ggtccccgcc tgtaccgcaa gattcgccgc
     5941 gacaccgccc tgcaggacct tgttgtaatc ggtcatgccc ggatcggtgc cgttggtcgc
     6001 ggtggccgga gcattgaaat tgaagcagtc ctgaagcagg tcgtcgctac tgaaggtgag
     6061 ccattcgcag tcatcgtctc ccggtatggt catatcatgc ttgccgagga gacattcgtc
     6121 cccgctggtc gggcggctcc a
//
LOCUS       DAOQUE010000118         6115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_14246_length_6115_cov_4.155776, whole genome shotgun sequence.
ACCESSION   DAOQUE010000118 DAOQUE010000000
VERSION     DAOQUE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14246_length_6115_cov_4.155776"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PKH10_04940"
     CDS             complement(<1..581)
                     /locus_tag="PKH10_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27511.1"
                     /translation="MLEHRRIPDADMFSFTHAGQPWVNWEWAHNILSALLWMHLGPAG
                     IIFFRLLTAFLLCLVLFALFRRLSDGDLFLSFSSVLTVFLLVQQRVSDRPQFFAYLLI
                     AAVVYLATTLQDIRSGRAAVLWGILVFLRNAHPSWVLAVLLVAVLVADRWLSRETHLT
                     GKETALLLFGLVSVVFATPQPLVAGHYLSLQFGP"
     gene            complement(706..2097)
                     /gene="asnS"
                     /locus_tag="PKH10_04945"
     CDS             complement(706..2097)
                     /gene="asnS"
                     /locus_tag="PKH10_04945"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNT27512.1"
                     /translation="MSYLLLKDLFKRHGECMNTAVSVAGWVRTLRVSKNLGFIELNDG
                     SFFSGVQIVFQDTLSNFEAVSKLGTGSAIAVTGTVVASPGKEQSFEIAATAIEIVGAC
                     DSDYPLQKKRHSFEYLRTIAHLRPRSNTFSAVFRVRSLLAHAIHSFFQDRGFVYVHTP
                     IITGSDCEGAGEMFRVTTLDPKSPPLTEEGKVDFSKDFFGKETNLTVSGQLAVENFCM
                     AFRDVYTFGPTFRAENSNTTRHASEFWMIEPEIAFAGIEEDMRLAEDMTKFIIRFVLE
                     RAPEEMKFFNAMIDKGLLERLEQVASSDFGRVTYTDAIDLLKKSGKTFDYPIEWGSDL
                     QTEHERYLTEEVFKKPVFVIDYPKEIKAFYMRQNDDGKTVAAMDMLVPGIGELIGGSQ
                     REERHDRLLRRIEELGLKKEDYWWYLDIRRFGGCPHAGFGLGFERAVMYLTGMTNIRD
                     VQPLPRTPKNAEF"
     gene            complement(2153..3871)
                     /locus_tag="PKH10_04950"
     CDS             complement(2153..3871)
                     /locus_tag="PKH10_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27513.1"
                     /translation="MPLPMRPLMPNRRNAFIIFFFVTLLVTGVLSYHEIGCDDHWWHV
                     ATGRLIRAEMRVPDVDVFSFTYAGSSWHNHEWGFSLFISLVWDYAGPVGFFLYRWLVL
                     FGIVAFLWGAVREHRVRAGASPPSLSFSFSLLFFILLVIQIRIAIRPHLLGYLFLSGL
                     VWRLQRPLVWKDRWLLLGQSLLFFAAWSFFHYSWVLGVVAVVTTIGDRYLRWKWGDGI
                     RFLKDMLRLARQYRWRVIAISSSLLILMTLLLFYGPLSFVLSHFFVLDNNEWKSYLYW
                     LSLHPFFAVTIPYYLLLILFSLRIIRTEPFYGFLVFIVAILPFRSSRFMTEFLIISLP
                     IMIHYLSEFLFDRDLRLLRRIPVVMLNAILAVALAESTVLMYRDWGVGVSTTKNPVWL
                     ADFMEEKGLAGNLYATNLSFHAYFMFRSWPEIKVFFDGRDMQVYPVYFIKDYLSLSFS
                     EIVTKYPVDYIVKDIAISGLDRHDAEFLAAAPSPYELVYFDQHWALFMKEEVIARYPA
                     IRSFRMLYPINLASERLMRQVVERGNLPILRGELAHYLTLVRQPEQQQLYDLMRAEIA
                     KYGPVD"
     gene            complement(3831..4946)
                     /locus_tag="PKH10_04955"
     CDS             complement(3831..4946)
                     /locus_tag="PKH10_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNT27514.1"
                     /translation="MIQEKIYAFFVERKGWIPLGFFLIFLFILVLLPHISAILLAAFF
                     TAYAVSPLVAFMNNRMRLPRSVATILLMILALLFLALLVFVILPAIFAQLADAVKRLP
                     LLAVSTVNWVYEKAYENGINISEYQNLTEEALLERLGTVVPSFQSMPQFMGTVFRQTF
                     SALFFLVDLLIYAVVTFFVSTRLPAVYDALVSLFPPARKEEMVEWLHKFDRVLAGFIR
                     GQLGVCFVLGSLYAFGLTVIGLPDGASLGILIGTLCFVPYIGIITGIIVASLLALSAG
                     GPLLFVKVLIVFAVIQMTDMIAITPNIVGRNVGVSPVFVIIALFAGAELAGFLGVLVA
                     VPTFAILKLIGTYFVEKYKASAIYNAPADAPTNAQPS"
     gene            complement(4948..5679)
                     /locus_tag="PKH10_04960"
     CDS             complement(4948..5679)
                     /locus_tag="PKH10_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNT27515.1"
                     /translation="MLLVSLCVALAVCASTLGAAECGAAGAVKVDKLEGTAKSSPDQQ
                     AWTPLKSGQEVPASHFVKTESASRLMLIFPDGSALKVGENSLVSLTEIVSNSSCERES
                     YKMKVFFGKIWSNVKKLVSKNEAGGFVVESKNAVAGVRGTSFGFVVETNGAGALMVEN
                     GKVEIAVPAKPAEPKKMNIQEWKKNRSKQEVAGPKEIGVQEWEKIVVEAMQMVKFSAD
                     GTLSKPVPIGAANADAWYRENAPVK"
     gene            complement(5728..>6115)
                     /locus_tag="PKH10_04965"
     CDS             complement(5728..>6115)
                     /locus_tag="PKH10_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27516.1"
                     /translation="VLGSSAGQAAQAHIDPVTGGTMAIDKAAKNASQKLVTDMVKSWT
                     QTLNNGEEFTLVVGKLPDSVAMFKAEQAVRANKLVTGVVVQSFEGGTATFRVMFRGDA
                     NGLAQSLADKFKATGIDGKKVVVEPK"
BASE COUNT         1510 a         1721 c         1751 g         1133 t
ORIGIN      
        1 ggcccgaact gtaacgagag gtaatgcccc gcgaccagag gttgcggggt cgcgaagacc
       61 acggaaacga gcccgaacaa caggagtgcg gtctcctttc ccgtcaggtg tgtttcgcgg
      121 gaaagccatc ggtccgcgac caacaccgcg acaagaagca ccgccagtac ccaggaggga
      181 tgcgcgttgc gcagaaagac cagtatcccc cagagcacgg cggcgcgccc cgacctgata
      241 tcctgcaggg tcgtcgcgag atagacgacc gcggcgatca acagataggc aaagaattgg
      301 ggtcggtcgc tgacgcgctg ttgcaccaga agaaataccg taaggaccga agagaaggaa
      361 aggaacaggt cgccgtccga aagacgacgg aacaaagcaa aaagcaccaa gcaaagcagg
      421 aaagcggtga gaagacggaa aaagatgata ccggcgggac cgaggtgcat ccacagcaac
      481 gccgaaagga tattgtgcgc ccattcccag ttgacccacg gctgtccggc atgggtgaaa
      541 ctgaacatat cggcatcagg tatccggcgg tgttccagca tgtccaaccc ctgtgcgagg
      601 tggacccaga aatcacagtt cggtataaga tatatcgtca acaccacaca gtacaaaaga
      661 aaagccgtgc cgagaaggag atactgaagg aaaggcctct tcccgtcaga actcggcatt
      721 cttgggtgtc cgggggagag gttggacatc ccggatattg gtcatgccgg tgaggtacat
      781 gacggcccgt tcgaaaccga gaccgaaccc ggcgtgcgga cagccgccga agcgacggat
      841 atcgaggtac caccagtaat cttccttttt cagaccgagt tcttctatgc ggcgcagaag
      901 acgatcatgc cgctcttcgc gttgagaacc accgatgagt tcgccgatgc cggggacgag
      961 catgtccatc gcggcgacgg tcttgccgtc gtcgttctgg cgcatataga aggcctttat
     1021 ctctttcgga taatcgatga cgaacaccgg cttcttgaag acctcttcgg tgaggtagcg
     1081 ttcatgttcg gtctggagat cgctccccca ctcgatcgga tagtcgaagg tctttcccga
     1141 cttcttgagc aggtctatcg catcggtata ggtgacgcgg ccgaaatcgg atgatgcgac
     1201 ctgttccagc cgctcgagca gccccttgtc gatcatcgcg ttgaagaact tcatctcttc
     1261 gggggcgcgc tccagcacga aacggatgat gaacttggtc atgtcctcgg ccagtcgcat
     1321 atcctcttcg atgccggcaa aggcgatctc cggctctatc atccagaact cggaggcgtg
     1381 gcgcgtggtg ttggaattct cggcgcggaa tgtcggaccg aaggtgtata catcgcggaa
     1441 cgccatgcag aaattctcga cggcaagttg gcccgacacc gtcaggttgg tctccttgcc
     1501 gaaaaagtcc ttcgagaagt ccaccttccc ttcttcggtg agcggcggcg acttcgggtc
     1561 gagcgtggtc acgcggaaca tctcccctgc cccttcgcaa tcggatccgg tgatgatggg
     1621 ggtatggaca tagacgaacc cgcggtcctg aaagaacgaa tggatggcgt gggcgagcag
     1681 gcttcgtact cgaaacaccg ccgagaaggt gttgctgcgc ggccgcagat gggcgatggt
     1741 ccgcaggtac tcgaaggaat ggcgcttttt ctggagcgga taatcgctgt cgcaggcgcc
     1801 gacgatctcg atggcggtgg ccgctatctc gaacgactgc tccttgccgg ggctcgcgac
     1861 caccgtgccg gtgacggcga tagcgctgcc ggtacccagt ttcgagaccg cctcgaagtt
     1921 ggaaagcgta tcctgaaaca ctatctggac acctgagaag aacgacccgt cgttgagttc
     1981 gatgaaaccg agattcttcg acacgcgcag ggtacggacc cagccggcga cagaaacggc
     2041 agtgttcata cattcgccat ggcgtttgaa aagatccttc agcaagaggt atgacatgaa
     2101 actccccctg tgggaaaaaa ccgtgacggt cagttatacc agagaacgac gattagtcaa
     2161 caggcccgta cttggctatc tcggcccgca tgaggtcata gagttgctgt tgttcgggtt
     2221 gccgcacgag cgtcagatag tgggcgagtt ccccccgaag gatgggcaga ttgccccttt
     2281 cgaccacctg ccgcatgagt cgctcgctgg caagattgat tgggtagagc atccgaaacg
     2341 accgtatcgc aggataccgt gcgatgacct cctccttcat gaacagggcc cagtgctggt
     2401 cgaaatagac cagttcatac ggtgaaggag cagccgcaag aaactcggcg tcatgtcgat
     2461 caagaccact aatagcaata tctttcacta tataatctac cggatatttc gtgacgatct
     2521 cagaaaaaga caatgacaga taatctttta taaaataaac aggatagacc tgcatgtccc
     2581 gaccatcgaa aaacactttt atttccggcc aggagcgaaa catgaagtaa gcgtggaaag
     2641 aaagattggt cgcatacaaa ttgccggcaa ggcctttctc ttccatgaag tcggccaacc
     2701 ataccgggtt ctttgtcgta cttacgccaa ccccccaatc tcgatacata agtacagtgc
     2761 tttccgccag agcgaccgca agaattgcgt taagcatgac aacagggatc cgacgcaaca
     2821 atcgaaggtc gcgatcaaac aagaattcgg acaaataatg gatcatgatc ggtaaggaaa
     2881 taatgagaaa ttccgtcatg aagcgactgc tcctgaacgg gagaatcgct acgataaaaa
     2941 ccaagaagcc gtaaaaaggt tcagttcgga tgatcctcaa agaaaagagt atcagaagca
     3001 gatagtacgg gatcgtcacc gcaaagaagg gatggagcga gagccagtac agataggatt
     3061 tccactcatt gttgtcaagc acaaaaaaat gagataaaac gaaagaaagg ggtccgtaaa
     3121 aaagaagcaa ggtcatcagg atcaaaagag aagaggaaat cgctatcacc cgccatcggt
     3181 attgtctggc gagacgcaac atgtctttca gaaagcggat cccgtcgccc cacttccatc
     3241 gcaggtatcg gtcccctatc gtcgttacca ccgcaacgac gccaagcacc catgagtagt
     3301 ggaaaaaaga ccacgccgcg aaaaataaaa gagactgacc aagtagtaac cagcgatcct
     3361 tccagacaag gggacgctgt agccgccaca caagcccgga aaggaagaga tatcccagaa
     3421 gatgcggtct aatggctatg cgtatttgta taacgagcaa gatgaaaaat aacagggaaa
     3481 aagaaaagga aagcgaaggt ggagacgctc cggcgcgaac tcgatgctct cgaaccgcgc
     3541 cccatagaaa ggccactatc ccgaataata ccagccaccg gtacaggaag aaaccgaccg
     3601 gcccggcgta atcccacacg agtgagatga agagactgaa accccactca tgattatgcc
     3661 acgatgaacc cgcgtaagta aaactgaaca catccacatc gggcacacgc atctcagccc
     3721 ttatcaggcg ccctgtggcg acatgccacc agtgatcatc gcacccgatc tcgtgatagg
     3781 aaagcactcc tgtcacgagc agggtgacga aaaagaaaat gatgaaagcg ttacgacggt
     3841 tgggcattag tgggcgcatc ggcaggggca ttgtagatgg cgctcgcctt gtacttctcg
     3901 acgaagtagg tcccgatgag tttcagtatc gcgaaggtcg gcaccgcgac gagcacaccg
     3961 aggaaaccgg ccagttcggc gccggcgaag agcgcgatga tgacgaagac cgggctgacg
     4021 cccacattgc gcccgacgat attcggagtt atcgcgatca tgtcggtcat ctgaatgacg
     4081 gcgaacacga taagcacctt gacgaaaagc agagggccgc cggcgctgag cgccaatagc
     4141 gacgcgacga tgatgccggt gatgatgccg atgtatggta cgaagcagag cgtgccgatg
     4201 aggatgccga gcgaggcgcc gtcgggcagg ccgatgaccg tgagcccgaa ggcatagagc
     4261 gaaccgagca cgaagcagac gccgagttgg ccccggatga acccggcgag cacccggtcg
     4321 aacttatgga gccactcgac catctcttcc ttccgggccg gagggaacag ggaaacaagc
     4381 gcgtcataga ccgccgggag ccgggtgctc acaaagaagg tcaccaccgc gtagatgagc
     4441 aggtccacga gaaagaagag cgccgagaag gtctggcgga acaccgtccc catgaactgg
     4501 ggcatcgatt gaaaactcgg caccaccgtg ccgagccgtt cgagtagcgc ctcttcggtg
     4561 agattctggt attcgctgat gttgatgccg ttctcgtacg ccttctcgta tacccaattg
     4621 acggtggata cggcgagcag cggcagtcgc ttcaccgcat cggcgagttg cgcgaatatg
     4681 gcgggaagta tcacgaagac aaggagcgcg aggaacagga gcgccaatat catgagcagg
     4741 atcgtcgcca cgctgcgcgg caggcgcatc cggttgttca tgaaggcgac gagcggcgat
     4801 acggcatagg cggtgaagaa ggcggcgagc aggatggcgc tgatgtgcgg caggagtacg
     4861 aggatgaaga ggaatatcag aaaaaaaccg agcggtatcc agcccttacg ctcgacaaaa
     4921 aacgcgtata tcttctcttg tatcatatca cttcaccggc gcgttctcgc ggtaccacgc
     4981 atcggcgttg gcagccccga tggggacggg tttggaaagc gtcccgtcgg cggagaactt
     5041 gaccatctgc atcgcctcga cgacgatctt ctcccactcc tgcaccccta tctccttcgg
     5101 tccggcgacc tcctgtttcg aacggttctt cttccattcc tgaatgttca tcttcttcgg
     5161 ctcggccggc ttggcgggca ccgcgatctc gaccttgccg ttctcgacca tgagcgctcc
     5221 ggcgccgttc gtctcgacca cgaagccgaa actggtgccg cgcaccccgg cgacggcgtt
     5281 cttcgactcg acgacgaagc cacccgcctc gttcttgctg accagtttct tcacattcga
     5341 ccatatcttg ccgaaaaaga ccttcatctt gtaactctcg cgttcacagg aggagttcga
     5401 cactatctcg gtcagactga cgagcgagtt ctcacccacc ttgagcgccg agccgtcggg
     5461 gaatatcagc atgagccggg aggccgactc ggtcttgacg aaatgggagg cgggcacctc
     5521 ctgccccgac ttcaggggcg tccatgcctg ctggtcgggc gacgacttcg ccgtcccttc
     5581 cagtttatcc accttcacgg cgccggcggc gccgcactcc gccgcgccga gcgtcgatgc
     5641 gcagacggca agggcgacgc acaaggaaac aaggagcata ttccgcacga tcccctcctt
     5701 caggaaaacc ttcggtgcca tgatagacta tttgggctcg accacaactt ttttcccgtc
     5761 gatgccggtc gccttgaatt tgtcagccag cgactgcgcg agcccgttgg cgtcgccgcg
     5821 gaacatgacg cggaaggtcg ccgtgccgcc ttcgaacgac tgcaccacca cgccggtcac
     5881 cagtttgttg gcgcgcaccg cctgttccgc cttgaacatc gcgaccgagt cgggcagttt
     5941 gccgaccacg agcgtgaact cctcgccgtt gttgagcgtc tgggtccacg acttcaccat
     6001 gtcggtcacc agtttctgcg acgcgttctt cgccgccttg tctatcgcca tggtgccgcc
     6061 ggtcaccggg tcgatgtggg cctgcgccgc ctgccccgcc gacgagccga gcacc
//
LOCUS       DAOQUE010000119         6110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_14258_length_6110_cov_3.987118, whole genome shotgun sequence.
ACCESSION   DAOQUE010000119 DAOQUE010000000
VERSION     DAOQUE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14258_length_6110_cov_3.987118"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1956
                     /locus_tag="PKH10_04970"
     CDS             274..1956
                     /locus_tag="PKH10_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27517.1"
                     /translation="MRLFTRCAMCVFALLPALLSANPTRELIGTAEGVNPFSARILPQ
                     GPETAYYNPALLLDVKDNVTISFSYLYQGLGITLFDRPAGSDILGDMNANDGFGSGIY
                     LGRVVRDGKPDRQLPFKAQPTGVLAGRGSESPEAHGYYATVGAVGAIIEEWLSLGFTM
                     ILPLTELQGQRSFFVDEREANFSNSLHFELYGDRLQGFSITFGLGGRVYWEWLTAGVG
                     VSLINSTQITAEAYVPDAARNEKDVLARSSIGLRATPHFAIAARPWEKLRIAATLHLS
                     SHNEIDSDIDMDFWRMQGTMENSVGQSAFSIVHAYLPLNLSLAFALGRFDLDGVGLTA
                     TLTGSYERWSDYRNRQNARPQDNLYWDYDLETEYTDEATGQPSTLYGGFVKETIPGFA
                     WKDIFKVAVGVAMDYEKNRWGLDVAFTPSPVPEQSGRTNYVDNHRLSFGGGYSRNWEI
                     GDYSFDTGVGVQCHYLFDRTQNKVTGVPATAAATKTGTGKSGTVVDEFPESVSDYDGT
                     HLVESDGFQTNNPGYPGYRSDGLLVSAGVWLTFYLDKKKEENPKDKDMEKKE"
     gene            1953..2981
                     /locus_tag="PKH10_04975"
     CDS             1953..2981
                     /locus_tag="PKH10_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27518.1"
                     /translation="MKKASVLFCALLFIVSCGDESTGPQTDTNTVPDESAVTDDTVTD
                     EAVVDEDAATGESDLPDESDLSDDAVDPSDQTIIPDDTTDGLLSDEETDGEVPDDTDP
                     DCQPGAYPEVCGDWAQQMIFTATAKVAGFDAATAEIRTLFRMTQRQQGGHILVDSKIC
                     HIVIENKTALNPIKILMPQSFADALIMLHKEADIELDGTYYQAPYWELRSIKQSILPA
                     DPGTYELPTEPTDPDVEDWDNDSIPGLRVNVNAGLSNGVTHIVEKSSSELWGTVKGTG
                     ETQTIDGTVHWTDKQVVLSTDNVLFKGGAQNVIKEEGPNIWRQKCIPANWTCTEVMAN
                     GDTLFPPL"
     gene            complement(2991..3590)
                     /locus_tag="PKH10_04980"
     CDS             complement(2991..3590)
                     /locus_tag="PKH10_04980"
                     /inference="COORDINATES: protein motif:HMM:NF025044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNT27519.1"
                     /translation="MDHGIIRQLVRSLRALPGVNQRQAERIAFTLLQDRTARAALDEI
                     VSTLSLTDPCPRCGWFIEREQPCANCGGEHSVICVVENFSDLLGIERTGQFAGSYHLL
                     GGLIAPLEGTLPEDLNAEPLRERVEAGGIAEVILALPATIEGEATIHYLHELLAPTGV
                     TLTRIARGIPSGSKVEYLDRKTVGNAIAERVKVSSGLSE"
     gene            complement(3598..5208)
                     /locus_tag="PKH10_04985"
     CDS             complement(3598..5208)
                     /locus_tag="PKH10_04985"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNT27520.1"
                     /translation="MQARRLFFAMVVIALAVFSCSSPEEDDPGTDDLGGADTAGNADD
                     IQNPDETGDDGATDALESDGDQLQTEELPDVESLIQCEPGKTDECYHGPSGTRDRGIC
                     KAGLATCGASGYWGPCEGEVLPQPEICHNDIDENCDGEDGTEDNTDDYDGDGYTYCTG
                     DCCEFTSECTNPTLVNPGAYDFPDNQIDDNCNDQVDEVVSCDTGLAEDSTDPVEMARS
                     IGICPPEEGKDYGLVTAEMLFPDGTTTSQAAQVPGADSQGNAITVDCTPGPPNPDSYA
                     ILPKFGSVLVPHEGERFFMMSSGIADDPIPPAAMSDQNISTETMCTRSNAPTDWYAAN
                     GSKFPSSPSCGAAGDPGKEPLNDPVMLQVKVKVPLNARSFSVDIYFSSREFPEYVCEY
                     NDFFVALLDSTYTSSDPNLQNPTDKNLAMDEAGNPVGINLAPAGLFRVCCQGGALQCG
                     YDPLHNYAQFCTLGPDELTGTGLYDGDDKNGATGWLVTKGNVVPGEEITLRFAIWDAL
                     DHVLDSEVILDNFRWYPVEVKPGTSIQQ"
     gene            complement(5232..5822)
                     /locus_tag="PKH10_04990"
     CDS             complement(5232..5822)
                     /locus_tag="PKH10_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27521.1"
                     /translation="MVHRSFFFLFIALAAVTGRAVEDSTEAKLFHNEGYFTHEQVRQG
                     HAEYDRTVAEMHTLRSSTGAAEFATAAKLITDLDRRMRNSNDLVQKLVAYYKQKWNQK
                     HYQGMDKKWKEETYAKHKAKITSLQVLIQHLGAAYNDWNAARNSTVGLKDRKKTLSIF
                     TRANGELSKALDGIAQTEAETFQALESELAAAKRMK"
     gene            complement(5825..>6110)
                     /locus_tag="PKH10_04995"
     CDS             complement(5825..>6110)
                     /locus_tag="PKH10_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNT27522.1"
                     /translation="HEKLDGSGYPHGKKGDDIGVVERILSLADMYDALTAADRSYKKA
                     LPREVALRILGDEVAAGKLDRRIFEVFRDHIDEIDAFVKERMQQHSGEAH"
BASE COUNT         1128 a         1915 c         1863 g         1204 t
ORIGIN      
        1 cgaaaaacga gagggacata atggcgcgac tcatgatagg cctccttgcc acacttattt
       61 cggataactc ccacaagttg ctagaatgat tactcagaaa aacctttttt gcaagaaaaa
      121 agggaccttc atatctccaa acaaacgaaa atctaaccgc atactgcttg ctcccattta
      181 tgacacctga gttcataaac agcacataaa ttcttgacta tcttttacct tttgataaca
      241 tcgcgccact gacaaacgac gatccgaggg atcatgcggc tctttacccg ttgcgccatg
      301 tgcgttttcg cacttctccc cgccctcctt tccgccaacc cgacccgcga actcatcggc
      361 accgccgagg gggtgaatcc cttcagtgcc cgcatactcc cgcagggtcc cgagaccgcc
      421 tactacaacc cggcactcct gctcgatgtc aaggacaatg tcaccatctc tttctcctac
      481 ctgtatcagg ggcttggcat aacgctcttc gaccgtcccg ccgggagcga catcctcggc
      541 gacatgaacg cgaacgatgg cttcggatcg ggcatctacc tcgggcgggt cgtccgcgac
      601 ggcaagccgg accggcaact tcccttcaag gcccaaccga ccggggtgct cgccggtcgc
      661 ggctccgaaa gccccgaggc ccatggctac tacgccacgg tcggcgcggt cggcgccatc
      721 atcgaagagt ggctctcgct cgggttcacg atgatactgc cgctcaccga actgcaggga
      781 caacgctcct ttttcgtcga cgagcgcgag gcgaacttct ccaattcgct ccacttcgaa
      841 ctgtacggcg atcgtctgca gggcttctcg ataacgtttg gtctgggtgg tcgtgtgtac
      901 tgggaatggc tcaccgccgg ggtgggggtg tcgctcatca acagtacgca gataacggcc
      961 gaagcctatg tccccgatgc cgcccgcaac gaaaaggatg tcctcgcccg ctccagcatc
     1021 ggcctgcggg cgacgccgca cttcgcgata gccgcccggc cgtgggaaaa actgcgcatc
     1081 gccgcgacgc tccatctctc ttcgcacaac gagatagatt ccgatatcga catggatttc
     1141 tggcggatgc agggaacgat ggagaactcg gtcggtcaga gcgccttctc gatcgtccac
     1201 gcctacctgc cgctcaacct ctcgctcgcc ttcgcgctcg gccggttcga cctcgacggc
     1261 gtggggctca ccgcgacgct taccggttcc tatgaacggt ggagcgacta ccgcaaccgg
     1321 cagaacgccc ggccgcagga caatctctac tgggactacg acctcgaaac ggagtatacc
     1381 gacgaggcca ccggtcagcc gtcgaccctc tacggcggct tcgtgaagga gacgataccg
     1441 ggcttcgcgt ggaaggacat ctttaaggtg gcggtcggcg tcgcgatgga ttatgagaag
     1501 aaccgctggg ggctcgatgt cgccttcacc ccgagcccgg tgccggaaca gagcggtcgg
     1561 accaactatg tggacaacca tcgtctctcg ttcggcggcg gctactcccg gaactgggag
     1621 ataggcgact actcgttcga taccggcgtc ggggtgcagt gtcactacct gttcgaccgg
     1681 acgcagaaca aggtgaccgg tgtgccggcg accgccgccg ccaccaagac cggcaccggt
     1741 aagagcggca cggtggtcga cgaattcccc gaatcggtga gcgactacga cggcacgcac
     1801 ctcgtggaat cggatggatt ccagaccaac aaccccggct accccggcta ccggagcgac
     1861 ggcttgctcg tcagcgcggg ggtatggctc accttctatc tcgacaagaa aaaagaggaa
     1921 aaccccaagg acaaagatat ggagaaaaag gaatgaaaaa agcgtctgta cttttctgcg
     1981 ctctgctctt catcgtttcc tgcggcgatg aatcgaccgg cccgcagacc gacacgaaca
     2041 ccgtccccga cgagtcggcg gtgaccgatg acaccgtgac cgacgaggcg gtggtcgacg
     2101 aagatgccgc gaccggagag tccgacctgc cggacgagtc ggatctgtcg gacgacgcgg
     2161 tcgatccgtc ggatcagacg atcatccccg atgacacgac cgatggactg ctatccgacg
     2221 aggagacgga cggcgaggtg ccggacgaca ccgatcccga ctgccagccg ggcgcctatc
     2281 ccgaggtctg cggcgactgg gcccagcaga tgatattcac cgccaccgcg aaggttgccg
     2341 ggttcgatgc cgcgaccgcc gagatacgga cgctgttccg gatgacccag cgccagcagg
     2401 gcggtcatat cctggtcgac tcgaagatat gccacatcgt catcgagaac aagacggcgc
     2461 tcaacccgat aaagatactg atgccgcagt cgttcgccga cgcgctcatc atgcttcaca
     2521 aggaggccga catagaactc gacggcacct actatcaggc gccttactgg gaactgcgct
     2581 cgataaagca gtcgatactc ccggccgacc ccggcaccta cgaactgccg accgagccga
     2641 ccgaccccga tgtcgaagat tgggacaacg acagcatccc gggcttgcgg gtcaatgtca
     2701 acgccggcct gtcgaacggg gtgacccaca tcgtcgagaa atcgtcgtcc gaactctggg
     2761 gcacggtgaa ggggaccggc gagacgcaga ccatcgacgg taccgtccat tggaccgaca
     2821 aacaggtggt gctctcgacc gacaatgtgc tgttcaaagg cggtgcgcag aatgtcatca
     2881 aggaggaggg acccaacatc tggcgacaga aatgcatccc ggccaactgg acctgcaccg
     2941 aggtcatggc gaacggcgac acgctcttcc cgccgctctg atacccgaag ttattccgac
     3001 agccctgaag acaccttgac ccgttcggcg atcgcgttgc cgaccgtctt gcggtcgaga
     3061 tattcgacct tcgaccccga cgggatgccc cgcgcgatgc gggtcagcgt gacgccggtc
     3121 ggcgccaaga gttcgtgcag gtagtgtatc gtcgcctccc cttcgatggt ggccggcagt
     3181 gcgaggatga cctccgcgat gccgcccgcc tcgacgcgtt ccctgagcgg ctccgcgttc
     3241 aggtcctccg gcagggtccc ctcgagcggc gcgataagcc cgccgagcag gtggtaggac
     3301 ccggcgaact gtcccgtccg ctcgatgccg agcaggtcgg agaagttctc gacgacgcag
     3361 atgacgctat gttccccgcc gcagttcgcg cagggctgtt cccgttcgat gaaccagccg
     3421 catcgcgggc aggggtcggt gagcgagagc gtcgagacga tctcatcgag agcggcgcgt
     3481 gccgtccggt cctgcaggag cgtgaacgcg atcctctccg cctgtcgctg gttcacgccg
     3541 ggcagagcgc ggagcgaccg gacgagttgc cggatgatgc catggtccat gaggcggcta
     3601 ttgctggatg ctcgtccccg gcttcacctc gaccgggtac cagcggaagt tgtcgagtat
     3661 gacctccgaa tcgaggacat ggtcgagcgc gtcccatatc gcgaaccgga gcgttatctc
     3721 ctcgcccggc accacattgc ccttcgtgac gagccaaccg gtcgcgccgt tcttgtcgtc
     3781 gccgtcgtaa agcccggtgc cggtcagttc gtcggggccg agcgtgcaga actgcgcgta
     3841 gttgtgaaga gggtcatacc cgcactggag ggcgccaccc tgacaacaga cgcggaacag
     3901 ccctgccggc gcgaggttga tgccgaccgg attgcccgcc tcgtccatcg cgaggttctt
     3961 atcggtgggg ttctggaggt tcgggtcgct tgaggtgtag gtcgaatcga ggagcgcgac
     4021 gaagaaatcg ttgtactcgc acacatattc ggggaactcg cggctcgaga agtagatgtc
     4081 gacgctgaac gagcgggcgt tcagcggcac cttcaccttg acctgcagca tcaccgggtc
     4141 gttgagcggt tccttgcccg ggtcgcccgc cgcgccgcag gagggggaac tcgggaattt
     4201 gctgccgttg gcggcatacc agtcggtcgg tgcgttcgag cgggtgcaca tggtctcggt
     4261 tgatatgttt tggtcactca ttgcagctgg cggtatcggg tcgtcggcga tgccggagga
     4321 catcatgaaa aagcgctcgc cctcatgcgg caccagcacg ctaccgaatt tcgggagtat
     4381 cgcgtaggaa tcgggattgg gcggacccgg tgtgcaatcc acggtaatgg catttccttg
     4441 agagtccgct cccggaacct gagccgcttg gctggtcgtc gtgccgtcgg ggaaaagcat
     4501 ctcggcggtc acgagcccgt aatcctttcc ctcctcgggc gggcagatgc ctatcgaccg
     4561 cgccatctcg accggatcgg tcgaatcctc ggcgagcccg gtgtcgcacg atacgacctc
     4621 gtccacctga tcgttgcagt tgtcgtctat ctggttgtcg gggaaatcgt acgcgccggg
     4681 gttgacgagc gtgggattgg tgcattccga ggtgaattcg caacagtcgc cggtgcagta
     4741 ggtgtacccg tcgccgtcgt agtcgtcggt gttatcctcg gtgccgtcct cgccgtcgca
     4801 gttctcgtct atgtcgttgt ggcagatctc gggttgcgga aggacctcgc cttcgcaggg
     4861 gccccagtag ccgctcgcgc cgcaggtggc gagccccgcc ttgcagatac cgcggtcacg
     4921 ggtgccgctc ggcccgtggt aacattcgtc ggtcttgccc ggctcgcact ggatgagact
     4981 ctccacatcg gggagttcct cggtctgtaa ttggtcgccg tcgctttcca gcgcgtcggt
     5041 cgcgccgtcg tcgcccgtct cgtcggggtt ctggatgtcg tcggcgttcc ccgccgtgtc
     5101 ggcgccgccg agatcgtcgg tgccgggatc gtcctcctcg ggagaggaac aggaaaagac
     5161 cgcgagagcg ataacgacca tcgcaaaaaa taaccgccgt gcctgcatgg tgacgcctcc
     5221 gtgccgcttt tttatttcat ccgttttgcc gccgccagtt ccgattcgag cgcctgaaag
     5281 gtctcggcct cggtctgcgc tatcccgtcc agcgccttcg agagttcccc gttggcgcgc
     5341 gtgaatatcg aaagtgtctt tttccggtcc ttgagcccga ccgtgctgtt gcgcgccgcg
     5401 ttccagtcgt tgtaggcggc gcccagatgc tggatgagca cctgcaggga ggtaatcttc
     5461 gccttatgct tggcgtaggt ctcctccttc catttcttgt ccattccctg atagtgcttc
     5521 tgattccatt tctgcttgta gtaggcgacc agtttctgca ccagatcgtt gctgttccgc
     5581 atgcggcggt cgaggtcggt gatgagtttc gccgccgtcg cgaactcggc ggcgccggtc
     5641 gaactcctga gcgtatgcat ctcggcgacg gtgcggtcgt actcggcgtg tccctgccgc
     5701 acctgttcgt gggtgaaata cccctcgttg tggaagagtt tcgcctcggt ggaatcctcc
     5761 accgcgcggc ccgttacggc tgccagtgcg atgaagagga agaagaagga acggtgtacc
     5821 atgctcagtg cgcctcccct gagtgctgtt gcattctttc tttcacgaac gcgtcgatct
     5881 cgtcgatatg gtcgcggaac acctcgaata tccgccggtc gagttttccc gccgccacct
     5941 cgtcgccgag tatgcgcagg gcgacctcgc gcgggagggc ttttttgtag gaacggtcgg
     6001 cggcggtcag cgcgtcgtac atgtcggcga gcgacagtat ccgttcgacg acgccgatat
     6061 cgtcgccttt tttgccgtgc gggtagccgc ttccgtcgag tttctcgtgg
//
LOCUS       DAOQUE010000120         6043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_14448_length_6043_cov_5.573480, whole genome shotgun sequence.
ACCESSION   DAOQUE010000120 DAOQUE010000000
VERSION     DAOQUE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14448_length_6043_cov_5.573480"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1464)
                     /gene="dnaA"
                     /locus_tag="PKH10_05000"
     CDS             complement(127..1464)
                     /gene="dnaA"
                     /locus_tag="PKH10_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNT27523.1"
                     /translation="MWKKIKEKLKASENDYLIKAFINPLRPIKESEEELVIGCPSQQI
                     ITLLEKNKLVHKISQVARDLVSPDIAVRFIVKKLDDLENIQPLLPLSEAVEEGTARGK
                     QNKQKSTPLSTEYTFSTFVAGPSNRDVYETALAVAKHPGTNRNPLVIYGGTGLGKTHL
                     LQAIGAFAASKNKAACYVPAQKLINEFYKAMSEKGMQKFQETLLSFDILLLDDIQFMK
                     TPGIQDEFFRIFNDYHAAKKQIVITSDRYPGEIREIDDRLKSRFEWGVTIEIRPPDLD
                     TRIAIVRKKTDLYGMAMDEEAVFFIAKNFKNNVRQIEGALKTLQISAEIVGRKEVTKD
                     FAIDRLRDMIRTKTHITIDEVMKMTATIMGIKVSDLCSKDRSKQISLSRQIAMFLSKK
                     YTGMTLVDIGQRFGRDHSTVISSISRIEKMALNDTNIRKIIEKLERELEDMKY"
     gene            complement(1580..2323)
                     /locus_tag="PKH10_05005"
     CDS             complement(1580..2323)
                     /locus_tag="PKH10_05005"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNT27524.1"
                     /translation="MTDPLISFDRMYDRDTVIAQRRGGYRFSVDALLLSWFAHEYAPA
                     RVTRTLELGAGSGIVSILLKRRGLGGTVDAIERQESLFSLLAHNVSLNHLEASVFPIL
                     KDIRMLSAFHERYDLVCFNPPYRAADSGRTAPDEERAAARHELFGSLDDFLRIGGAAL
                     KKKGRLFFVYPVARLAFACLSTAAHGLYPRQIVLVSESPADPPSLALFCCAPGDVRRS
                     KLDLSLVTMREKDGAESAVASKILHASSC"
     gene            complement(2316..2945)
                     /locus_tag="PKH10_05010"
     CDS             complement(2316..2945)
                     /locus_tag="PKH10_05010"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNT27525.1"
                     /translation="MDFFSPEEAAWKLRNGAVVAYPTETVYGLGAIIHETEAVLRLKQ
                     VKGILRTKPLSVLITKNRSSMLDELVTSFCPIAQRLAKFYWPGPLTIVHEARPSVPDL
                     ITGGSGFVGIRCSPHPGLNKLLDLVGHPIITTSANLAGKPPATNYEEVFAYFADRIDG
                     VLVFDEKQSEEKTPASNIPSTIVRVDAQKVEILREGAIKKEDLLRKIYD"
     gene            complement(2959..3447)
                     /gene="purE"
                     /locus_tag="PKH10_05015"
     CDS             complement(2959..3447)
                     /gene="purE"
                     /locus_tag="PKH10_05015"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNT27526.1"
                     /translation="MTHPTVLILMGSDSDWPVMKKAADILSEFGVIYEAHVSSAHRTP
                     ERTVALVTGFCGACIIVGAGAAAHLGGVVAAHTVKPVIAVPLNATGLGGLDALLSTAQ
                     MPSGIPVASMAIDGAKNAALFAVSILACSDKDLADRLAVYRVTMAREVKAKDARLQET
                     AK"
     gene            complement(3466..3855)
                     /locus_tag="PKH10_05020"
     CDS             complement(3466..3855)
                     /locus_tag="PKH10_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27527.1"
                     /translation="MEALAIGEASLAYRLISLSVSLAIANATDAGAITGLLDNVDNTL
                     HNGKTFLTAQNSGGLLTGEISGLFDSLLSCSAQVKEYAKNKNYDRLQKVRTCTEMIEG
                     SLSALTERFNRKEPASPPVTPVKKSGN"
     gene            complement(4007..4648)
                     /gene="rsmG"
                     /locus_tag="PKH10_05025"
     CDS             complement(4007..4648)
                     /gene="rsmG"
                     /locus_tag="PKH10_05025"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNT27528.1"
                     /translation="MAIDVSRETFRAALDSGLTELGLSLSEEVLQKSADLYALLLEWN
                     RVHNLTRISDPVEAAHRHFVESWASLLLPGLFDGVSSVADIGSGAGFPGLPLALARPD
                     TSFTLVEKAPKKAAYLSFAAASLKMENISIRCADARRLSDRFDLVTFRALTSDDRFVG
                     EMTPLLSPGGRLLLFLGPSQKIPSGLTGHERTIILSGKKTRFALLSIASTRSR"
     gene            complement(4638..>6043)
                     /gene="mnmG"
                     /locus_tag="PKH10_05030"
     CDS             complement(4638..>6043)
                     /gene="mnmG"
                     /locus_tag="PKH10_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNT27529.1"
                     /translation="IGEDSLPAGRLGEPAATFLTGALVAAGHTSLRLKTGTPVRLRGD
                     SIDFSSLEKQSGDDDFVPFSTATRDQLTRQVPCWITHTNERTHAIIRDNLHRSPLYGV
                     HKSIEGIGPRYCPSLEDKVVKFPHRERHQIFLEPEGWDQREYYPNGISTSLPYSVQEE
                     FLRTISGLEQAVITRPAYAIEYDAFDPRDLGRTLESKCMPGLFLAGQVNGTSGYEEAA
                     IQGLVAGINAAQRSLRNDEPPFVLERQEAYGGILIDDIVTKGIDEPYRMFTSRAEYRL
                     SLRDDNTAARLLEKGHRRGLLSGPRYVFERQRLDTEARLIAFLNDTVVSPTATVNTLL
                     SEFGEAPLIKPTSLGGLLRRPGFDLRMLARISPLPSADAEIWERAIISVRYAAYIDKE
                     MEERARTAQLTFVAIPSGFSFTGLPGISREIEEKLTRHRPGDLGQAARIPGVTPAAVS
                     ILFMHLKGRSEGGSRGD"
BASE COUNT         1312 a         1579 c         1805 g         1347 t
ORIGIN      
        1 tgtttatagt aacataatac tttatattta tttaatatac tatccattaa attcttcgta
       61 tcccttttat gtcttcgtgg gagttacaga taaacatttt tttaacaccg ctgttttgaa
      121 catttatcaa tatttcatat cctcgagctc tctttccagt ttttctatga tcttacggat
      181 gttagtgtca ttaagggcca ttttctctat gcgggagatg gatgatatga ccgtcgaatg
      241 atcgcggccg aaccgctgtc cgatatcgac cagggtcatg ccggtatatt ttttagaaag
      301 gaacatggct atctgacggg atagtgatat ctgtttcgag cggtcctttg agcagagatc
      361 ggataccttt atacccatga tggtggcggt catcttcatg acctcatcga tggtgatatg
      421 ggtcttagtg cggatcatgt cccgcaaacg gtcgatcgca aaatctttag taacctcctt
      481 gcgccccacg atctccgccg atatctggag ggtcttgagt gcgccctcta tctgcctgac
      541 attattctta aaattcttcg caataaagaa cacggcctcc tcatccatcg ccatgccgta
      601 caggtcggtc ttcttccgga caatggcgat gcgggtgtcc agatcgggcg gacggatctc
      661 gatggtgacc ccccactcga aacgggactt cagacgatca tcgatctccc gtatctcgcc
      721 ggggtaccgg tcggaggtga tgactatctg ttttttcgcg gcgtggtagt cgttgaatat
      781 ccggaagaac tcatcctgta tccccggtgt cttcatgaac tgtatgtcgt cgagcagcag
      841 gatgtcgaac gagagcaggg tctcttggaa tttctgcatc cccttctcgc tcatcgcctt
      901 gtagaattca ttgatgagtt tttgcgccgg tacataacag gccgccttgt tcttcgatgc
      961 cgcaaaagcg cctatagcct gcagcagatg ggtcttacct agaccggtgc cgccatagat
     1021 gacgagcggg ttgcgattcg tccccgggtg cttggcgacg gccaaagccg tttcgtaaac
     1081 atcccggttg ctagggccgg ccacgaaggt gctgaaggtg tattcggtgg aaagcggagt
     1141 gcttttctgt ttattctgtt taccgcgggc ggtcccctcc tccaccgcct ccgaaagagg
     1201 gagcagcggt tgaatgttct cgagatcgtc cagtttcttg acgatgaagc ggaccgcgat
     1261 atcgggggaa acgaggtcgc gggcgacctg tgatatcttg tggaccagtt tgttcttctc
     1321 gagtagtgtg atgatctgct ggctgggaca gccgataacc agttcctctt cactctcctt
     1381 gatggggcgg agaggattga tgaaggcctt gatgagataa tcgttctccg aggccttgag
     1441 tttctccttg atctttttcc acatgtggtc agtcggctcc tggtaacggt ccctgtggca
     1501 gtttccttat ctccctcttg cctgcacagt ctagtgatga ttttctcttt ttcaatttca
     1561 ttgaaagaaa aatcatcatt taacaagagg aggcatgtaa tatcttggag gcgaccgcgc
     1621 tttccgcccc gtccttctcg cgcatggtga cgagagaaag atcaagcttt gatctccgga
     1681 catcaccggg ggcgcaacaa aaaagtgcca gcgacggcgg gtcggcaggt gattcgctca
     1741 cgagaactat ctgccgcgga tagagcccat gggcagcggt cgacagacag gcgaaggcaa
     1801 gccgcgccac tggatagacg aaaaacaacc gccctttctt tttgagagcc gcgcccccga
     1861 tccggagaaa atcatccaac gagccgaaca actcgtgacg ggcggcggca cgctcttcgt
     1921 caggcgcggt cctgcccgaa tcggccgctc gatagggagg attgaaacag acaagatcat
     1981 atcgctcgtg aaaagctgat aacatcctga tatctttgag tatagggaag acactcgctt
     2041 cgagatgatt gagtgagaca ttgtgggcga gcaacgagaa gagggactcc tgtcgttcga
     2101 tcgcatcaac ggttccaccc agtccgcggc gcttgagaag tatcgagacg atgccgctcc
     2161 ccgcaccgag ttccaatgtc cgggtcaccc gcgccggcgc atattcgtga gcgaaccagg
     2221 acaacaaaag ggcgtccacc gagaaacggt atcccccgcg tcgctgtgcg atgacggtat
     2281 cgcggtcgta catacggtcg aaggagatca agggatcagt catatatctt gcgcaacagg
     2341 tcctctttct tgatggcgcc ctctcgtaat atctcgacct tttgcgcatc gacccgcacg
     2401 atggtggaag gtatgttgct cgctggggtc ttctcctcgg actgtttctc gtcgaaaaca
     2461 agcacgccgt ctatgcgatc ggcgaaatag gcaaagacct cctcgtagtt ggtcgcgggc
     2521 ggcttgccgg ccagattggc gctggtcgtg atgatgggat ggccgacaag atcgagcagt
     2581 ttattaagtc ccggatgagg ggaacaacgg ataccgacaa agccggaccc gcccgttatg
     2641 aggtcgggga cgctgggccg ggcctcgtgt acgatggtca gcggaccggg ccagtagaat
     2701 ttggcgagac gctgtgcgat ggggcagaag gaagtaacaa gttcgtcaag catgctggaa
     2761 cggttcttgg taatcaggac cgaaagaggt ttcgttctca ggatgccttt gacctgcttg
     2821 aggcgaagga ccgcctccgt ctcgtgaatg atggcgccga gaccgtatac cgtttcggtc
     2881 ggataggcaa cgaccgcgcc gttccgtagt ttccaggccg cctcttcggg agagaaaaaa
     2941 tccatcggcg ggccacacct atttcgctgt ttcctgtaga cgggcgtctt ttgccttgac
     3001 ctcccgggcc atggtgaccc gatagaccgc gaggcggtcg gcgagatcct tgtcggaaca
     3061 tgcgaggatg gataccgcga acagagcggc gttcttggca ccatcgatgg ccattgacgc
     3121 cacagggatg ccggagggca tctgcgcggt cgaaaggagc gcgtcgagtc cgccaagacc
     3181 ggtcgcgtta agcgggacgg cgatgaccgg cttgaccgta tgggccgcca ccacgccgcc
     3241 gaggtgggcg gcggctccgg cgccgacgat gatgcaggcc ccacagaagc cggtgacgag
     3301 agcgaccgtc cgttccggtg tccggtgagc cgaggagaca tgtgcttcat agatgacgcc
     3361 gaattccgag aggatgtcag ccgctttttt catgacaggc caatcggaat cacttcccat
     3421 gaggatcagg acggttggat gggtcatacc gctctccttt ttattttagt tgccgctttt
     3481 tttcaccggt gtcacgggcg gagacgcggg ctctttccgg ttaaatctct ctgtcagcgc
     3541 ggaaagggag ccttcgatca tttcggtgca ggtacgaacc ttctgaaggc ggtcataatt
     3601 cttgttcttg gcatactctt tgacctgtgc cgagcaggaa agcagggagt cgaaaagacc
     3661 gcttatctcg ccggtcaaca ggccgccgga gttctgggcg gtcaggaagg tcttgccgtt
     3721 atgcagagtg ttgtccacat tgtccagaag accggtgata gcgccggcgt cggtggcgtt
     3781 cgcgatggca agactgaccg aaagggagat cagacgatag gcgagcgagg cctctccgat
     3841 cgcgagagcc tccatccggc gcgtaagggg agcgggctct tccgctgaac agaaaagatg
     3901 agtaaggaag aacaatagcc caatgaacaa ggttgttttt cgcatgtttc gccctcctct
     3961 tgaacacgaa gaaacagggc gaggataaca gaaaagaaaa ggcctgtcaa cgcgaccggg
     4021 tagaagctat gctcaaaaga gcgaaccggg tctttttccc ggagagaata atggttcgtt
     4081 cgtggccggt gagcccggaa gggatctttt gggacgggcc aaggaacaaa agcagtcgac
     4141 caccgggaga aagcaggggg gtcatctcgc cgacgaatcg atcgtcggat gttaacgcgc
     4201 ggaaggttac gaggtcgaaa cggtcggaaa gtctccgggc gtcagcgcat cggatcgaga
     4261 tgttttccat tttaagtgag gcggcggcga aggagagata cgcggctttt ttgggagcct
     4321 tctcaacgag cgtgaaggaa gtgtcggggc gagccagtgc aagcggcaga ccggggaacc
     4381 ctgcgcccga accgatgtcg gcaacggaag agacaccatc gaaaagcccc ggcaggagaa
     4441 gcgaagccca cgattccacg aaatgacgat gcgccgcttc gacggggtcg ctgatgcggg
     4501 tcaggttgtg gacgcggttc cactcgagga ggagcgcata cagatcggcg cttttttgaa
     4561 ggacctcttc cgaaagagag aggcccaatt cggtcaaccc cgaatcgagc gcggcccgaa
     4621 aagtttcacg tgaaacatca atcgccacgg gaacctcctt cggaacgacc tttcagatgc
     4681 atgaagagga tcgagaccgc cgctggggtg acccccggta tccgagccgc ctgtccgaga
     4741 tcgccgggac ggtgacgggt caatttttcc tctatctcgc gcgatattcc cggcagaccg
     4801 gtaaaggaaa agccggacgg tatcgcgaca aacgttagtt gggcggtccg cgcccgttct
     4861 tccatttcct tatcgatgta agcggcataa cggaccgaaa tgatcgcccg ttcccagatc
     4921 tccgcgtcag ccgaaggaag aggagaaatg cgcgccaaca tccttagatc gaacccggga
     4981 cggcgcagaa ggccaccgag agaggtgggc ttgatgagcg gagcctcgcc gaattcggaa
     5041 agaagagtgt ttaccgtagc ggtaggggag acgacggtgt cgttcaaaaa ggcgattagt
     5101 cgagcctctg tatcgagtcg ttgccgctcg aagacatagc gtgggccgga tagaagtccg
     5161 cgccggtgcc ccttttcgag cagacgggcg gcggtgttgt cgtctcgcag agaaagacga
     5221 tattcggcgc gactcgtgaa cattcgataa ggctcgtcga tccctttggt gacgatatca
     5281 tcgatcagta tcccgccgta cgcctcctgc ctctcgagaa cgaaaggggg ctcgtcattt
     5341 ctgagagagc gttgtgccgc gttgatccca gcgaccaatc cctgtatcgc cgcctcttcg
     5401 tagcccgagg tgccgttcac ctgtcccgcg aggaacagcc ccggcataca tttactttcc
     5461 agcgttcgac cgagatcgcg tgggtcgaac gcgtcgtatt ctatggcata ggcagggcga
     5521 gtgatgaccg cctgttccag tcccgatatg gtccgcagga actcttcctg cacggagtaa
     5581 gggagactgg tcgaaatgcc gttcggataa tattcgcgtt ggtcccatcc ctccggctca
     5641 aggaagatct gatgtcgctc gcggtgaggg aacttcacca ctttgtcctc cagcgaaggg
     5701 cagtagcgcg gaccgatccc ttctatgctt ttgtgaacgc cgtaaagggg agaacggtgg
     5761 agattgtcgc ggatgatcgc atgggtccgc tcgttggtgt gggttatcca gcaagggacc
     5821 tgtcgggtca gctggtcgcg tgtcgcggtg gagaagggga cgaaatcgtc gtcgcctgat
     5881 tgcttttcaa gagacgaaaa atcgatggaa tcgccgcgca ggcgtaccgg cgtaccggtc
     5941 ttgagtctta aggaggtatg ccccgccgcg acgagtgcgc cggtaagaaa ggtcgctgcc
     6001 ggttcgccga gccgtcccgc ggggagagaa tcctcgccga tga
//
LOCUS       DAOQUE010000121         5932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_14789_length_5932_cov_4.079122, whole genome shotgun sequence.
ACCESSION   DAOQUE010000121 DAOQUE010000000
VERSION     DAOQUE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14789_length_5932_cov_4.079122"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKH10_05035"
     CDS             <1..540
                     /locus_tag="PKH10_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNT27530.1"
                     /translation="FAGAAVVHIEGYLVQDHALLEQALSIARQSGARVSLDLASHNVV
                     SENIEFLRSLAARRLIDILFANEEEAQAFAGRPPEEALPMMASTCPTVIVKLGKEGSL
                     VSRNGITTRIAAVPAPCLDTTGAGDLYAAGFLYGLLRELPSDRCGAIGSLLASRVICR
                     VGAKMDDREWGDIRRSLGI"
     gene            537..2993
                     /locus_tag="PKH10_05040"
     CDS             537..2993
                     /locus_tag="PKH10_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNT27531.1"
                     /translation="MTRILVLDVSAYLFRAYHALPPMHAPDGSPVNAIYGCAAMFLRV
                     AKRFGTFRAVAALDCGRDTVRRERYPDYKANRKELDEELRVQFAHVPEMLEALGLTAM
                     AVPGYEADDVIAAIRKAHPGDEVIVVSSDKDLMQLIDERTFLYDGVKERIVDAIAVAE
                     KFGVPPSKLRDLLAMSGDSSDNIPGLPGVGPKTAAKLLSEYADIEDIYAHLDALSPKL
                     RDKFVNFRDQLMLSRELVSLIPDIALPPLPAEPWIGPDPDRFRAFIERFGFRSLARHL
                     EVTRTPAQPISSTGTVPTDDGDNMPEGYLFADDGVIYLAMDGVIQETSAGELPVDRHW
                     FGFDLKAFIPAGIEDRHRLTDLQLAYFILDSGRHGYDIGEVIDYLGTAPIEGEGPFLT
                     LARHKAIRDAVMPQIQGDERRRELLYGIEMPHLAAVRDMERHGIAVDRERLVAMRDEY
                     TAKLADIERQAHEWAGTPVNLNSPKQLAELLFVKLGLPAGRKTKSGRSTDSDVLEALA
                     PYHPLPGLILEHRQFQKLLSTYLEPIAQKVSPDGRLRSTFILTHAATGRLASRDPNLQ
                     NIPVRTEEGRRIRSLFPAAPGHRLISLDYSQIELRILAALSQDEELLGAFRNGEDIHR
                     KTASAIFGVFPELVDDAMRRHAKAVNFGIIYGMQAFKLSQDTGVEIKFAQEYIDQYFR
                     FYTGVKRFIDATLAQARSDGWVETLHGRRRSVPELLSDNRITARAGERMAFNTVIQGT
                     AAEIMKRGVVQARAALAAAGIPAKLLLAIHDEIILESPADHADRAAQVAADALRSAGK
                     GLPVPLEVAISSAERWDLLD"
     gene            complement(3001..3444)
                     /locus_tag="PKH10_05045"
     CDS             complement(3001..3444)
                     /locus_tag="PKH10_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNT27532.1"
                     /translation="MLTNVRRTKKKEKPYTEVVTDQRNELEKDPIVRELIDLTNYQKP
                     KQRTECLKMKRPCLFVSCKYHLYLDVNPDTKSIKFNFPGKEVWELRETCSLDVADKGG
                     VTLEEVGAIMNLTRERIRQVEMRALQKLKTNGAKYNIKNLFFANK"
     gene            3723..4628
                     /locus_tag="PKH10_05050"
     CDS             3723..4628
                     /locus_tag="PKH10_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27533.1"
                     /translation="MRFSGMVAMLVLGGAMTLVISCASQQAESGDQSVAEKEIALYTE
                     GVKDKDVALLVKLFDLKEKYPEEIDEAVKARFNALYTNLPKNSLDSFKESPNQTLRVA
                     AGQSRLHLKNNYTSGNTLIFIREGGKLIINEVLPFNTERQIDLKPDTYEFIVVIDRKF
                     NNNKYLYTTTLNMTDGTIYVGEFNIASELNPSGDCPAGRVRDDDTCMKVEFRAFDNCA
                     PGTHLVDYLCCDEGYNFIMDGKCSRYTDKVDTVMCPAGYEEGGSGRCCPKGMKFIDGR
                     CQNPPPGWVPPDQPQAAPAEEKAAE"
     gene            4781..5779
                     /locus_tag="PKH10_05055"
     CDS             4781..5779
                     /locus_tag="PKH10_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNT27534.1"
                     /translation="MLLHATLFLIRFDLGGAGGDPLMPVQLLDDVSLAATTEKERPDP
                     ATAPPAPSEQEKIDPPAGQIVDIARPENEKRPEESRFLAKYDSTVPREKRSVFDKDIP
                     IRGKKLQRMALPPTAKVTPQRDELVTPGNAPEHREKKEELVRKGDIAGFDGTEGELSK
                     GAEKETPGRIDGDAPVDRKGQEAAELSVPLRYLPYFLGDQTMLTSPSNDYLKDIPEED
                     ETALNARRFLYADYYNRIKKAVSYYWTPGKVLMVHDPGGNIFGAQDRYTKIEAVLDER
                     GRIVDIQVTRPSGADVLDREAVDAFKAAAPFPNPPAPLLRNGRLAIQFGFYVSIDR"
BASE COUNT         1262 a         1903 c         1664 g         1103 t
ORIGIN      
        1 ttcgccggcg ccgccgtcgt tcatatcgag ggatatctcg tgcaggacca cgcgctcctc
       61 gaacaggcgc tgtcgatcgc ccggcaaagc ggcgcacgcg tctcgctcga tctcgcgagc
      121 cacaatgtcg tttccgagaa tatcgagttc ctccgttcgc tcgccgcgcg acggctcata
      181 gacatcctct tcgccaacga ggaggaggcg caggccttcg ccggtcgtcc gccggaagag
      241 gcgctcccta tgatggcctc gacctgcccc accgtcatcg tcaaactcgg caaggagggc
      301 tccctcgtct cccgcaacgg catcacgaca aggatcgcgg cggtgccggc cccctgcctc
      361 gacacgaccg gcgcgggcga tctgtatgcc gccggcttcc tctacggact cctgcgcgag
      421 ctcccgtcgg ataggtgcgg cgcgatcggt tctctcctcg cctcgcgggt catctgccgc
      481 gtcggcgcca agatggacga ccgcgagtgg ggggatatac ggagatccct cgggatatga
      541 cacgcatact cgtgctcgat gtctcggcct acctgttccg tgcctaccat gccctgccac
      601 cgatgcatgc ccccgacggg agcccggtca acgccatata cggctgcgcc gccatgttcc
      661 tgcgcgtcgc caaacggttc gggaccttcc gggcggtggc ggcgctcgat tgcggtcgcg
      721 acacggtgcg ccgcgaacgc taccccgatt ataaggcgaa tcgcaaggaa ctcgacgagg
      781 aactgcgcgt ccagttcgct catgtcccag agatgctgga agcgctcgga ctcaccgcga
      841 tggcggtgcc gggctacgag gccgacgatg tcatcgcagc gatacgcaag gctcaccccg
      901 gcgacgaggt gatcgtcgtc agttccgaca aggacctgat gcaactcatc gacgagagga
      961 cctttctcta cgacggggtg aaagagcgga tcgtcgacgc gatcgcggtg gccgagaaat
     1021 tcggggtgcc gccctcgaaa ctgcgcgacc ttctcgcgat gagcggtgac tcctccgaca
     1081 acatccccgg cctccccggg gtcggtccca aaacggcggc gaaattgctt tccgaatacg
     1141 ccgacatcga agacatctat gcccacctcg atgcgctttc accgaaacta cgggacaagt
     1201 tcgtgaactt ccgcgaccag ttgatgctgt cgcgcgaatt ggtctcgctc attcccgaca
     1261 tcgctctgcc gccactcccc gccgagccgt ggatcgggcc cgaccccgac cggttccggg
     1321 ccttcataga acgattcggc ttccgttcac tggcccgcca tctcgaggta acgaggaccc
     1381 ccgcacagcc gatatcgtcg accggaacgg tgccgaccga tgacggcgac aatatgcctg
     1441 agggctacct gttcgccgac gacggcgtta tctatctagc gatggacggc gtcattcagg
     1501 aaacgagcgc cggagagtta ccggtcgacc gccactggtt cggcttcgat ctcaaagcct
     1561 ttattcctgc agggatcgag gaccggcacc gcctgaccga tctgcaactt gcctacttca
     1621 tcctcgacag cggcaggcac gggtacgaca tcggggaggt catcgactac ctgggaacgg
     1681 cgcctatcga gggtgaaggt ccctttctca cccttgcccg ccacaaggcg atccgcgacg
     1741 ccgtcatgcc gcagatacag ggtgacgaac ggcgacgtga actcctatac ggcatagaga
     1801 tgccccacct tgcggcggtg cgagacatgg agcgccacgg gatcgcggtc gaccgcgagc
     1861 gacttgtggc gatgcgcgat gagtacacgg cgaaactcgc ggacatagaa cggcaggccc
     1921 acgaatgggc gggcacgccg gtcaatctga actcacctaa acagttggcc gaactcctgt
     1981 tcgtgaaact cgggctcccg gcgggacgca agaccaaaag cggccggtcg accgacagcg
     2041 atgtgctgga ggcgctggcc ccctatcacc ccctgccggg gctcatactc gaacaccggc
     2101 agttccagaa gctgctctcg acctaccttg agccaatcgc gcagaaggtc tcgcccgacg
     2161 gacgactccg ttcgaccttc atcctcaccc atgccgcgac cggtcgactc gccagccgcg
     2221 atccgaacct ccagaacatc ccggtccgca ccgaggaagg acggcgcatc cggtcgcttt
     2281 tccccgccgc acccggccat cgcctgatat cgcttgacta cagccagata gaactccgca
     2341 tcctcgccgc actttcgcag gatgaagaat tgctcggcgc cttccggaac ggcgaggaca
     2401 tacaccgcaa aacggcctcc gcgatcttcg gcgtattccc cgaactcgtg gacgacgcga
     2461 tgcgccgcca cgccaaggcg gtcaacttcg gcatcatcta cggcatgcag gcgttcaaac
     2521 tgtcgcagga caccggcgtg gagataaaat tcgctcagga atacatcgat cagtacttcc
     2581 gcttctacac cggcgtgaag cggttcatcg acgccaccct cgcacaggcc cgttctgacg
     2641 gctgggtgga gaccctgcac ggacggcgac gctcggtccc cgaactcctg agcgacaacc
     2701 ggataacggc gcgagcaggc gaacgaatgg cattcaacac cgtcattcag ggcacggcgg
     2761 ccgagatcat gaagcgaggc gtcgtgcagg cccgggccgc tctcgccgcc gccgggatac
     2821 cggcaaaact attgctcgcc atccatgatg agataatcct cgaatcgccc gccgatcacg
     2881 ccgaccgcgc cgcgcaggtc gccgccgacg cactccgcag tgccggcaaa ggacttccgg
     2941 tcccgcttga ggtcgcgata tcttcggctg aacgatggga tctgctcgac tgaacgacgg
     3001 ctacttattg gcgaagaaaa gattcttgat gttgtatttc gcgccattgg tcttgagttt
     3061 ttgcagggcg cgcatctcaa cctgacgtat cctctctcgg gtcaggttca tgatggcacc
     3121 gacctcctcg agggtgacac cgcccttgtc ggcgacatcg agcgaacagg tctcacgcag
     3181 ttcccagacc tctttaccgg gaaaattgaa cttgatcgac ttcgtatcgg gattgacatc
     3241 gagatagagg tggtacttgc aagagacgaa caggcatgga cgcttcatct tgaggcattc
     3301 ggtgcgttgt ttgggtttct gatagttcgt gaggtcgatg agttccctaa cgatagggtc
     3361 cttttccagt tcgttacgct ggtcggtgac cacctcagtg tagggcttct ccttcttttt
     3421 cgttctcctg acattggtca gcattaaaaa gcctcctttt ttgggtccgg aagagttgtg
     3481 agtcccggaa acttttcaca tcctaacgca ccacgggccg ctgtcaacaa gaaatttata
     3541 accaacctta tttattagac atattttttg ttttccaaaa aacagatttc aaataccttc
     3601 tcaccgtccc ctccgttaga aagatggaaa gtagaaaaaa atgtatcgta atgttgattt
     3661 taccgtcact catcgctacc atgagaccgt tgcagagttc cacagtttaa atggagggaa
     3721 acatgagatt cagcggtatg gttgcgatgc tggtattggg cggtgcgatg acgctcgtca
     3781 taagttgcgc gtcccagcag gccgaatcgg gcgaccagtc ggtggccgaa aaggagatcg
     3841 ccctgtacac cgagggggtg aaggacaagg atgttgctct cttggtgaag ctgttcgacc
     3901 tcaaggagaa gtatcccgaa gagatagatg aagcggtcaa ggctcgcttc aatgcgctct
     3961 acacgaacct gccgaagaac tcgctcgact ccttcaagga atcgccgaac cagaccctgc
     4021 gtgtcgccgc gggccaatcg cggttgcacc tcaagaacaa ctacacgagc ggcaacaccc
     4081 ttatcttcat ccgcgaagga gggaaactca tcatcaacga agtgctgcca ttcaacaccg
     4141 agcggcagat agatctcaag ccggacacct atgaattcat cgtcgtcatc gaccgcaaat
     4201 tcaacaacaa caaatacctc tacaccacca cgctcaacat gaccgacggc acgatctatg
     4261 tcggcgaatt caatatcgcg tcggaactga accccagcgg cgactgcccc gcaggccgcg
     4321 tacgcgacga cgacacctgc atgaaggtag agttccgcgc gttcgacaac tgcgcccccg
     4381 gcacccattt ggtcgattac ctttgctgcg acgagggata taattttatc atggacggca
     4441 aatgttcccg ctacaccgac aaggtcgaca cggtcatgtg tcctgccggt tacgaggagg
     4501 gcggatccgg ccggtgctgt cccaaaggga tgaagttcat cgacggtcgc tgtcagaacc
     4561 cccctccggg ctgggtgccg cccgatcagc cgcaggccgc ccccgccgaa gagaaagcgg
     4621 cggagtgacc ccggcctctc ctcttttgcg ccaaaaaaga acttcacaga acacttcttt
     4681 gatgctatga tcgcgtcatg gtcgatcgag atcgcaggca catcaaaaaa agaccgcagg
     4741 gcctgaccgc gccggcatct tgttctttct tgtcgtttcc gtgctcctgc atgcgacgct
     4801 cttcctcatc cggttcgacc tcggcggagc aggcggtgat ccgctcatgc ccgtacaact
     4861 gctcgatgac gtctctcttg ccgccacgac cgagaaggaa cggccggatc cagccaccgc
     4921 tccgcccgcc ccctcggaac aggaaaagat cgatcccccc gccggccaga tcgtcgacat
     4981 cgcacgcccc gagaatgaga aacgccctga agagtcacgg ttcctggcga aatacgacag
     5041 cacggtaccg cgcgagaaac gaagcgtctt cgacaaggac ataccgatac gcggcaaaaa
     5101 actccagcgg atggcgctcc cccccaccgc gaaggtgacg ccgcaacgcg acgaactggt
     5161 gacgccgggc aacgcccctg agcaccgcga gaagaaagag gagctcgtac gaaaaggcga
     5221 catcgccgga tttgacggta cggagggaga actgtcgaaa ggggccgaaa aggagacgcc
     5281 gggccggatc gacggcgacg caccggtcga ccgaaaggga caagaagcgg cagaactatc
     5341 ggtaccactt cgttacctgc cctacttcct tggcgaccag acgatgctca cctctccctc
     5401 gaacgactac ctgaaggaca tccccgaaga ggacgagacg gcgctcaacg cacgacgttt
     5461 cctctacgcc gattactaca atcgcataaa aaaggctgtg agttactact ggacgccggg
     5521 caaggtgctt atggtgcacg atcccggcgg gaacatattc ggcgcgcagg atcgctacac
     5581 caaaatagag gcggtgctcg acgaacgggg ccggatcgtg gacatacagg tgacccgtcc
     5641 gtcaggcgcc gatgtgctcg accgtgaggc ggtcgatgcc tttaaggcag ctgcgccctt
     5701 tcccaacccg ccggccccgc tcctgcgtaa cggacgactc gccatccagt tcggttttta
     5761 tgtcagcata gaccggtgat cgcatgagta cgccccccgt catcgtcatc atcccgtcgc
     5821 gtctgctcga tacccagcgc ccgatactcg gcgcatcctt caccgaacgg gccgccgtca
     5881 cgatcgcccg atactacggg gtcgcgacgg tctacgctcc gcatctcccc tc
//
LOCUS       DAOQUE010000122         5886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_14953_length_5886_cov_4.914423, whole genome shotgun sequence.
ACCESSION   DAOQUE010000122 DAOQUE010000000
VERSION     DAOQUE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14953_length_5886_cov_4.914423"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="PKH10_05060"
     CDS             <1..1043
                     /locus_tag="PKH10_05060"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNT27535.1"
                     /translation="QHSVKELRAIGIQPDILLCRCEAELTNDLREKIALFTNVKKEAV
                     FSATDVKNIYELPLVYARQGVDDIIAKYLNIWSRSAGLKKWETICERINSPKGTVRIA
                     VVGKYVDLKESYKSLNEALHHGGIEHHHKVELCYVDSEKLEKGEIGVEALENCDGILV
                     PGGFGNRGIEGKIKAARYAREKGIPYFGICLGMQIAVIEFARHVCGMEQAHSTEFDAG
                     TKYPVIDLMEEQKQLKKLGGTMRLGAYTCRIESDSHAHRAYQNDLISERHRHRLEFNS
                     HFEKDLTEKGLRITGRDRETGLVEIVELPGHPYFLGCQFHPEFKSKPFAPHPLFAAFV
                     GAAIAYRGRHGR"
     gene            1033..1272
                     /locus_tag="PKH10_05065"
     CDS             1033..1272
                     /locus_tag="PKH10_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27536.1"
                     /translation="MGDKIEMGASAAPAPLKGSPHLRIGSLEPSRHHQLTARNEMRMF
                     LLLFMLALALLVAALSRMADPVGAVLDFVQPLIGY"
     gene            complement(1310..1966)
                     /gene="rpe"
                     /locus_tag="PKH10_05070"
     CDS             complement(1310..1966)
                     /gene="rpe"
                     /locus_tag="PKH10_05070"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014195417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNT27537.1"
                     /translation="MVKIAPSVLSADFTHLADEIRSIEKSGADIVHLDIMDGHFVPNI
                     TFGPFIVKQIRALTKLPLDAHLMIENPGQYLQAFKDAGSDWITVHVETERHLHRTLQQ
                     IRAIGCKAGVSLNPHTGLDWMASVADLMDLVLVMTVNPGFGAQKYIPAAAAKIRDIAA
                     FRAKHKLPFDISVDGGINEKTAPEVISLGADILVAGNAFFSRLAAERPSIVKTLRDGK
                     "
     gene            complement(2053..3288)
                     /locus_tag="PKH10_05075"
     CDS             complement(2053..3288)
                     /locus_tag="PKH10_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNT27538.1"
                     /translation="MTIKGDSTSNNRLNRSQREPDGYTLILGDISHVINAAKRSTARS
                     INCIMTAAYWMIGQRMVEFEQKGKSRAAYGEEIIERLSIDLSQKFGRGFSRQNIQQMR
                     QFYLLYPPERICQTSSGELENSSRTMICQTPSGIFPAAYPDISLSELARLFPLPWSAY
                     VRLLAVRNELARRFYETEALRGGWSVRQLDRQINSQFYERTALSRNKAVMLTKGLKAL
                     PEDGVLPEEEIKDPYVLEFLDLKDEYSESDLEEALIRHLEKFLLELGGDFCFIGRQKR
                     LRVGDEWYRVDLLFFHRRLRCLVIVDLKLGKFTHADAGQMHLYLNYAREHWARPDENP
                     PVGLILCARNDESLARYTLENLPNKVMASEYRTALPDEKKLAAEIERTQCLLQNRKRI
                     VLASSEKKKRSRKASDIPR"
     gene            complement(3602..4723)
                     /locus_tag="PKH10_05080"
     CDS             complement(3602..4723)
                     /locus_tag="PKH10_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27539.1"
                     /translation="MTKHLAILVTLVMVACSNNENKIIKLVSVSVGGSHTCGLKPSGE
                     LYCWGDNHHGQIGDGTIDDKNSPTRVGIESDWGSVSARREDHTCGVKVSGSLYCWGDN
                     QWSELGDGTENDRATPTRIGTESDWASVSACDFSTCGVKKSGELYCWGNNWFGRLGDG
                     TTATRKTPTRIGTDSDWQNVSVGGFHTCGVKQSGELYCWGENSHGQIGDGTIDDKITP
                     TRVGTESDWLSVSAGEVHTCATKESSELYCWGDNHHGQIGDGTINDKNSPTRVGIESD
                     WQNVSVGGFHTCGVKQSGELYCWGENMFCQLGDGTLDGKTTPTRVGTESDWLSVSAGH
                     VHTCALKESGELYCWGSNSHGQLGDGSEWERTPQPVREP"
     gene            complement(4737..>5886)
                     /locus_tag="PKH10_05085"
     CDS             complement(4737..>5886)
                     /locus_tag="PKH10_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HNT27540.1"
                     /translation="QQVIEGQQSTVHDKQREADGFPRRIRLGRLTKPLPGKKAQVVVV
                     PTTTEPKFYHDDSVTEDGEGGETGGSGEGREGEVLGETPAEPQEGEGQGAGQGEGGEH
                     DLTNEAYDIGKVLTEKFHLPNIKDKGKKRSFNKYTYDLTDRNRGFGQLLDKKATMRRI
                     VETNIQLGNLDPDEPIRPEDLVVGPRDQVYRILSPEKDFETQAVVFFVRDYSGSMEGR
                     PTELVSLQHLLLYSWLMYQYQNNVITRFILHDTEAKEVPDFYTYYKSSVSGGTRCAPA
                     FHLVNRIIEIERLAVDYNIYVFYGTDGDDWDNDGAELIDEMEKMLATVNRVGITVTKS
                     SESDTVVERTIERSGLLKQKPKLIRLDTLSAPDADEPRLIEGIRKLVE"
BASE COUNT         1192 a         1717 c         1641 g         1336 t
ORIGIN      
        1 cccagcacag cgtcaaggaa ctgcgcgcca tcggcatcca gcccgacatc ctgctgtgtc
       61 gttgcgaggc cgaactgacc aacgatctga gagagaagat agcgctattc accaatgtca
      121 agaaagaggc ggtcttctcg gccaccgatg tcaagaacat ctacgaactg ccgctcgtct
      181 acgcacggca gggggtggac gacatcatcg cgaaatacct caacatctgg tcgcgctcgg
      241 cgggactcaa gaaatgggag acgatctgcg agcggatcaa cagcccgaag gggacggtac
      301 gcatcgcggt cgtcggcaaa tatgtggacc tcaaggagtc gtacaagtcg ctcaacgagg
      361 cgctccacca cgggggcatc gagcatcacc acaaggtcga actgtgctat gtggattcgg
      421 aaaaactgga gaagggggag atcggggtcg aggctcttga gaactgcgac ggcatccttg
      481 tccccggcgg gttcggcaac cgcggcatcg aggggaagat caaggcggcc cgctacgccc
      541 gcgagaaggg gataccctat ttcggcatct gtctgggcat gcagatagcg gtgatagagt
      601 tcgcgcgcca tgtctgcggc atggaacagg cccattcgac cgagttcgac gcggggacga
      661 aataccccgt catcgacctc atggaagagc agaagcaact gaagaaactc ggcggtacca
      721 tgcgtctcgg cgcctacacc tgccgcatcg agtccgactc gcacgcccat cgcgcctatc
      781 agaacgacct gataagcgaa cggcaccgtc accggctcga gttcaactcc catttcgaga
      841 aggacctgac cgaaaagggg ttgcgcatca ccggtcgcga ccgggagacg gggctggtcg
      901 agatcgtaga actgcccggc catccttatt ttctcggctg tcagttccac cccgaattca
      961 aatcgaagcc gttcgcgccg catccgctgt tcgccgcctt cgtcggcgcg gcgatagcct
     1021 atcgaggacg ccatgggaga taagatagag atgggtgctt cggcggctcc ggcgccgctc
     1081 aagggctcgc cccacctgcg catcggttcg ctcgaaccga gccgtcacca tcaactcacc
     1141 gcgcgcaacg agatgcggat gttcctgctc ctgttcatgc tcgcgctcgc cttgctcgtt
     1201 gccgcccttt cgcgcatggc tgacccggtg ggggcggtgt tggatttcgt ccagccgctc
     1261 atagggtact gaccgctttt tcccgaaaag accgttttga tccggggcgt tacttcccgt
     1321 cgcgcagggt cttcactatc gagggccgtt cggcggcgag acgcgaaaag aatgcgttcc
     1381 ccgcgacgag gatatcggcg ccgagggata tgacctcggg ggcggtcttt tcgttgatgc
     1441 cgccgtcgac cgagatgtcg aacggcagct tatgcttcgc gcggaacgcg gcgatgtcgc
     1501 gtatcttcgc cgccgccgcc gggatgtact tctgcgcccc gaagccgggg ttgaccgtca
     1561 tcacgagcac gaggtccatg agatcggcga ccgacgccat ccagtcgagc ccggtgtgcg
     1621 ggttgagcga tactcccgcc ttgcagccga tggcgcgtat ctgctgaagc gtccggtgaa
     1681 ggtgccgttc ggtctcgaca tggaccgtta tccagtcgct cccggcgtct ttgaacgcct
     1741 gcaggtactg gccggggttc tcgatcatga ggtgggcgtc cagcggcagt ttggtgagcg
     1801 cccgtatctg cttcacgatg aacgggccga aggtgatgtt cgggacgaag tggccgtcca
     1861 tgatgtcgag atggacgatg tcggcgccgc tcttctcgat ggagcggatc tcgtcggcga
     1921 gatgcgtgaa atcggccgaa agcacggatg gagcgatctt caccatgggc aactccctat
     1981 caaagacacc cgattatagc gccgccgacg gacaaggcaa taataaggaa aagcaaaaaa
     2041 ccaacatctc catcagcgag gaatatcaga agcttttctt gatctctttt ttttctccga
     2101 cgacgcaaga acaattcttt ttcgattttg gagcaggcat tgagttcgct cgatctccgc
     2161 ggctaatttc ttttcgtccg gcagggccgt tctgtactcc gaagccatca ccttattcgg
     2221 taagttctcc agcgtatatc gcgccaacga ttcgtcgttg cgggcgcaga ggataagtcc
     2281 cacaggcggg ttctcatcag gcctcgccca gtgttcgcgg gcataattca aatacaaatg
     2341 catttggccg gcatcggcat gagtgaattt gccaagtttg agatcaacga taacgagaca
     2401 gcggagtcgg cggtggaaaa agagcagatc aacacggtac cattcatcac cgacgcgaag
     2461 tcgtttctgg cgaccgataa aacaaaaatc tccacccaac tccagaagaa acttctctag
     2521 gtgccggatc aaagcttctt ccagatcgct ctcggaatac tcgtctttga gatcgagaaa
     2581 ctcaagcaca tacggatcct tgatctcttc ttcaggaagg actccgtcct ccggcaaggc
     2641 cttcagtcct ttggtcagca tgacggcctt gttccgggaa agcgccgttc gttcgtaaaa
     2701 ctgggaattg atctgtcggt cgagttgtcg caccgaccat cctccccgta atgcctcggt
     2761 ctcataaaat cgccgggcaa gttcgtttct taccgcgagt aatcggacat acgccgacca
     2821 cggcagagga aaaagacggg ccagttcgga aagcgatatg tccggatatg cggctggaaa
     2881 aatgccagac ggtgtctggc aaatcatcgt tcgggaagaa ttttctaatt cgccagacga
     2941 tgtctggcaa attcgttccg gcggataaag aagatagaac tgcctcattt gttggatgtt
     3001 ttggcgcgag aaaccgcggc caaatttttg cgagaggtca atcgacaatc gttcgatgat
     3061 ttcttcacca tacgccgcac gagactttcc tttctgttcg aattcaacca tgcgctgccc
     3121 aatcatccag taggcggccg tcatgatgca gtttatcgaa cgggccgtcg atcttttggc
     3181 cgcattgatg acatgtgaaa tgtcacccag gatcaaagtg tagccgtccg gctctctttg
     3241 tgacctatta agccgattgt ttgaagtgga atctcctttt atcgtcatgg ggcgatcttc
     3301 accaagagca actccctacc aaagacgccc gattatagca ccgctgacgg acaaggcaat
     3361 aaaagcagtt ctaatatcgc atcaaggacc atgagtgtcg tgtggttcat ttttcgattt
     3421 cggcgaaaag atacaacaca tagagcggca ttcgccgtat ctttcccgac atcccggtcg
     3481 gtgccaccga tacctgaaga tttggcgtga ttatcacgaa tgaaattata tggttgatct
     3541 tatctttggc taaaaagata ggggttcccg ggaaaaaatc tagggggggg gcacccgaca
     3601 atcacggctc tcttaccggt tgcggtgttc tctcccactc gctcccatcg ccgagttgac
     3661 catgactgtt actcccccaa cagtagagtt cgccgctttc ttttaaggcg caggtatgaa
     3721 catggccagc agaaacgcta agccaatcgc tctcggtccc cacgcgggtc ggagtggtct
     3781 tgccgtcaag agtcccatcg ccaagttgac agaacatgtt ctctccccaa cagtaaagtt
     3841 cgccgctctg tttcaccccg caggtgtgga atccgccgac cgagacattc tgccagtcgc
     3901 tctcaatccc cacgcgggtc ggtgagtttt tgtcgttaat agtcccatct ccgatctggc
     3961 cgtgatggtt atccccccaa cagtagagtt cgctgctttc ttttgtggcg caggtatgaa
     4021 cctcgccggc agaaacgcta agccaatcgc tctcggtccc cacgcgggtc ggagtgatct
     4081 tgtcgtcaat agtcccatct ccgatctgac cgtgactgtt ttctccccaa cagtaaagtt
     4141 cgccgctctg tttcaccccg caggtgtgga atccaccgac cgagacattc tgccagtcac
     4201 tgtctgtgcc gatgcgggtc ggggtcttcc tggtggcagt tgttccatcg ccgagacgac
     4261 cgaaccagtt atttccccaa cagtagagtt ctccactttt cttcacgccg caggtggaga
     4321 aatcacaagc cgagacgctt gcccagtcgc tctcggtgcc gatgcgggtc ggagtggccc
     4381 tgtcgttctc ggttccatcg ccaagttcac tccattggtt atctccccaa cagtacagtg
     4441 agccgcttac cttaacccca caggtgtgat cctctcttct agccgaaacg ctcccccagt
     4501 cgctctcaat ccccacgcgg gtcggtgagt ttttgtcgtc aatagtccca tctccgatct
     4561 ggccgtgatg gttatccccc caacagtaga gttcgccgct tggttttaat ccacaggtgt
     4621 gacttccgcc gacagagaca cttaccaatt tgataatctt gttctcgtta ttactacagg
     4681 caaccatcac cagtgttaca agaattgcga ggtgcttggt catgccctcc ccctaattat
     4741 tccaccagtt tccgtatccc ttcgatgagg cgcggttcgt cggcgtcggg cgccgagagc
     4801 gtatcgagcc ggatgagttt cggtttctgc ttgaggagcc ccgaccgctc gatggtccgt
     4861 tcgacgacgg tgtcgctctc ggaacttttc gtcaccgtga tgccgacgcg gttgaccgtc
     4921 gcgagcatct tttccatctc gtcgatcagt tcggcgccgt cgttgtccca gtcgtcgccg
     4981 tcggtcccgt agaagacata gatgttgtaa tcgaccgcga gccgctctat ctcgatgatg
     5041 cgattgacca gatggaacgc cggggcgcac cgggtgccgc ccgacacgct cgatttgtag
     5101 taggtgtaga aatcggggac ctccttcgcc tcggtatcgt ggaggatgaa gcgggtgatc
     5161 acattattct ggtactggta catgagccat gaatagagga gcaggtgctg gagactcacc
     5221 agttcggtcg gccgtccctc catcgagccg gagtagtcgc ggacgaaaaa gacgaccgcc
     5281 tgcgtctcga aatccttttc gggcgacagg atgcggtaca cctgatcgcg tggcccgacg
     5341 acgaggtctt cggggcgtat cggctcgtcc ggatcgagat tgcccagttg gatgttggtc
     5401 tcgacgatac ggcgcatggt cgccttcttg tcgagcagtt gtccgaagcc gcggttgcgg
     5461 tcggtcagat cgtaggtgta cttgttgaat gaccgttttt tccccttgtc cttgatgttg
     5521 gggagatgga atttttcggt gagcaccttg ccgatgtcgt aggcctcgtt cgtcaggtcg
     5581 tgctcgcccc cttcaccctg cccggccccc tgtccctcgc cctcctgcgg ttcggcgggc
     5641 gtctcgccga ggacctcgcc ctcccgccct tcgcccgagc cgcccgtctc gcccccctca
     5701 ccgtcctcgg tcaccgagtc gtcatggtag aacttgggct cggtcgtcgt cgggaccacc
     5761 acgacctgcg cctttttccc cggcagcggc ttggtgaggc ggccgagtcg gatgcggcgc
     5821 gggaagccgt cggcctcgcg ctgtttgtcg tgcactgtcg actgttgacc ttctatcacc
     5881 tgttgg
//
LOCUS       DAOQUE010000123         5862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15030_length_5862_cov_3.332185, whole genome shotgun sequence.
ACCESSION   DAOQUE010000123 DAOQUE010000000
VERSION     DAOQUE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15030_length_5862_cov_3.332185"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKH10_05090"
     CDS             complement(<1..291)
                     /locus_tag="PKH10_05090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT27541.1"
                     /translation="MRLVIQIPCYNEAEHLPATIADLPKKVVGIDEITVLVIDDGSKD
                     DTSGVARSLGAKVVRHTANRGLAAAFMTGLQTAIELDADLKVGTPVADALGLN"
     gene            428..2050
                     /locus_tag="PKH10_05095"
     CDS             428..2050
                     /locus_tag="PKH10_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT27542.1"
                     /translation="MMKKFFAVAGVLVLLSVWGCGDGKSAIEKKLEAEAIQKAEEAKK
                     AEEEKAKKAEEEKKKKYIDEDAKPLPPVLESATKKDWGKVKELLSSGTAIDTKDERGR
                     TILMLAAVQNNEELFDFLVAKGADTNARSLEGETPLLMAAKAGNKKMVDYLIEKGADP
                     NTKDNFGRTTLMYAAASGNVDLVKLFLGKGAELNALDKYNHNALVFAVRENRTEAAKF
                     LLGKGAQVNIVKPLTEEEKKALEEAKQKAEKERKIKEAVYDQTEEKRDKTVLFWAVEH
                     GNLELVKLLIAKGAPVLVKDVKKRVETIIYETGKGDIEKTQEVVEETIRTAYERGQKN
                     LLMYAAEGGHLEVLKFLVSKGAKIEETDGETVRHPLFYVVAQLKGTPEKEKRLAEVTK
                     YLIEEGKKMYKHKRRLSKQEIMQERAKQKNKAWWDKNIQKLQWKEEDRKKIDIEEYDY
                     EGWTLLALAVMNDHFDVFKVLIDAGAQVNRKERKFGKTALMYSRAIKNTEMEKILLEK
                     GAKEGIVFDVDEDARQERIKKEIEEDIKKGKL"
     gene            2064..2549
                     /locus_tag="PKH10_05100"
     CDS             2064..2549
                     /locus_tag="PKH10_05100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT27543.1"
                     /translation="MIFRTTVRPDDPQRIRDIVTSTGFFYDHEIAVAVELCEENLAKG
                     EEKSGYFFVFADDETTGRTLGYACYGEIACTRQRYDLYWMGVHADARGKGIGKLLMKE
                     TDKAIAARGGEIVYIETSSREKYLPTREFYRSTGCKLEAELKDFYDNGDNKVIYTRRV
                     R"
     gene            complement(2546..3364)
                     /locus_tag="PKH10_05105"
     CDS             complement(2546..3364)
                     /locus_tag="PKH10_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HNT27544.1"
                     /translation="MTAASKKRLSPAHRTELKKAFATLEESTFAAKLSQLTFGATDRT
                     TKNLPAAIVRLTEKVVGPALTAALSVAVRTLGKNGSDPEGPVARHLHKAMVALSGATG
                     GAAGLPGLIAELPLTTVLMLRRIAEIARKSGEDLSDPAARLACLEVFALSGIGAAPEA
                     VGSTYYAARIALHGTIAQAARELSGAGGKLAGHAFSGLVSKIAAKFGVNATRLAATRV
                     VPVAGAASGAVLNTLFMDHYQELAEAHFTIRRLERQYGADTVRAAYERLRKKKV"
     gene            complement(3361..4122)
                     /gene="dapB"
                     /locus_tag="PKH10_05110"
     CDS             complement(3361..4122)
                     /gene="dapB"
                     /locus_tag="PKH10_05110"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNT27545.1"
                     /translation="MATDNTRRIALVGACGRMGRAITRRLSDEGFSVLPIEASGHPAQ
                     GKDHGASIGTGPSGVTVTTLADPALASCRAVVDFGSPESFRQALDRALAAGIPFLSGT
                     TGLIDADRALMATAAKKIPLFHSANMSYGVAVIAEILPRLAQLTAGMEVAIVEAHHSR
                     KKDAPSGTALKLGEILIAGRGKNDVAIHALRGGDVVGDHTITFFGDAERIELTHRAHD
                     RDIFAAGAVRALAWLLDRKGPGHYSMQDIVKDTAR"
     gene            complement(4112..5131)
                     /locus_tag="PKH10_05115"
     CDS             complement(4112..5131)
                     /locus_tag="PKH10_05115"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNT27546.1"
                     /translation="MNMRISVVGAGSWGTVLASVLAEKNAEVSLWCREPELVEAIDRD
                     HENSLFLPGIPIHPKVKATNDLDAAVTNRDMIVMATPAQHTRAILSGASYRFLHGSQL
                     VIASKGIENGTLKLMHQVAEEIIPESMHRNIFVLSGPTFARELGMKMPTAAVVAGRYI
                     EGLNAAQEFFSLPYFRVYRSPDVVGVEVGGAIKNVIAIGVGIVEGMRLGRNSQSALMT
                     RAIAEITRLAVKIGANPFTISGLSGLGDLILTCTGELSRNRQVGIRIGHGEKLSDILA
                     GMTMVAEGVATSISAYQLAQKMEVTMPIVETVYRVLHEGLDVRTAMSALMGRSLKEEI
                     YGYGD"
     gene            5287..5547
                     /gene="rpoZ"
                     /locus_tag="PKH10_05120"
     CDS             5287..5547
                     /gene="rpoZ"
                     /locus_tag="PKH10_05120"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNT27547.1"
                     /translation="MARVTVEDCLEKIPNRLELIILSAKRTRQLLDKAAPQVPNTKKN
                     KEPIVALREVAAGKIGLARVEAKPKRPHFRRRRIGKYSGAQN"
     gene            5547..>5862
                     /locus_tag="PKH10_05125"
     CDS             5547..>5862
                     /locus_tag="PKH10_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27548.1"
                     /translation="MALFPGFNTNIRHKNKIFHIQTEVISTGDKHSVSTLVYLEGRIF
                     HAVKGVLPPAEAIHRETAALVIVKQHKEAIKKLISNQLAAADSAALPRGTINIVEFTE
                     LYQ"
BASE COUNT         1236 a         1701 c         1875 g         1050 t
ORIGIN      
        1 gttgagaccg agcgcgtccg cgacgggtgt gccgactttg aggtcggcgt cgagttcgat
       61 cgccgtctgc aggccggtca tgaaagcggc ggcgagcccg cggttggcgg tgtggcgcac
      121 caccttcgcg ccgagcgaac gggcgacccc ggaggtgtcg tccttcgagc cgtcatcgat
      181 gacgagcacc gtgatctcgt cgatgccgac caccttcttc ggcaggtcgg cgatggtggc
      241 ggggagatgt tcggcctcgt tgtagcaggg tatctgtatc accagtctca tccgacgctc
      301 cccatgcggt acgatacccc gttatctatg ttaaaaagag gtaaaaagaa attttttgaa
      361 aaatattttt tatatgttat ggtgccacaa aatgatcgta tgcacaacct gatttaagga
      421 gtgagccatg atgaaaaagt ttttcgcggt cgcgggcgtc cttgtcctgc tctcggtgtg
      481 gggctgcggc gacggcaaga gcgctatcga aaagaaactc gaagccgagg ctatccagaa
      541 ggcagaggaa gccaagaagg ccgaggagga gaaggcgaag aaggccgagg aggagaaaaa
      601 gaagaaatat atcgacgagg atgccaagcc gctgccgccc gtgctcgaat cggcgaccaa
      661 gaaggattgg ggcaaggtca aggaattgct ctcctccggt accgcgatcg acacgaaaga
      721 tgagcgtggt cgcaccatcc tcatgctcgc cgccgtccag aacaacgagg aactgttcga
      781 tttcctcgtc gcgaagggag cggataccaa tgcccgttct ctcgaggggg agacgccgct
      841 cctgatggcg gctaaggcgg gcaacaaaaa gatggtcgat tacctcatcg agaagggcgc
      901 cgatccgaac acgaaggaca acttcggtcg cacgacgctc atgtatgccg ccgcgtcggg
      961 caatgtcgat ctcgtgaaac tcttcctcgg caaaggggcc gagctcaacg cgctcgacaa
     1021 gtacaatcac aacgcgctcg tattcgcggt tcgcgagaac aggaccgaag cggccaagtt
     1081 cctgcttggc aagggggcac aggtcaatat cgtcaagccg ctgaccgaag aggagaaaaa
     1141 ggcgctcgaa gaggcaaagc agaaggccga aaaggagcgt aagataaagg aagcggtcta
     1201 cgatcagacc gaggagaagc gcgataagac ggtgctgttc tgggcggtcg agcacggcaa
     1261 cctcgaactg gtgaaactgc tcatcgcgaa gggagcgccg gtccttgtga aggatgttaa
     1321 gaaacgggtc gagaccatca tatacgaaac cggcaagggc gatatcgaga agacgcagga
     1381 ggtggtcgag gagaccatcc gcaccgccta cgaacgcggc cagaagaacc tgctcatgta
     1441 cgccgccgag ggcgggcatc tcgaggtgct taagttcctc gtttccaaag gggcgaagat
     1501 agaggagacc gacggcgaga cggtacgtca tccgctgttc tacgtcgtcg cgcagttgaa
     1561 ggggaccccc gaaaaggaga agagactcgc cgaggtcacg aagtacctca tcgaggaagg
     1621 caagaagatg tacaagcata aacgccgtct ttccaaacag gagatcatgc aggagcgcgc
     1681 caagcagaag aacaaggcgt ggtgggacaa gaacatccag aaactccagt ggaaagaaga
     1741 ggaccgcaag aagatagaca tcgaggaata cgattacgag gggtggaccc tgctcgcgct
     1801 cgccgtcatg aacgaccatt tcgatgtgtt caaggtgctc atcgacgccg gcgcgcaggt
     1861 caaccgcaag gagcgtaagt tcggcaagac ggcgctcatg tacagtcgtg ccatcaagaa
     1921 caccgagatg gagaagatcc tgctcgaaaa gggcgccaaa gagggcattg tgttcgatgt
     1981 ggacgaggac gcccggcagg agcgcatcaa gaaagagata gaggaagata tcaagaaagg
     2041 caaactctga tagccgcatc cccatgatat tccgcaccac cgtccgtccc gatgacccgc
     2101 agcgcatccg cgacatcgtc acctcgaccg gctttttcta cgaccacgag atagcggtcg
     2161 cggtcgaact gtgcgaggag aaccttgcga agggcgagga gaagagcggg tacttcttcg
     2221 tgttcgccga cgacgagacg acggggcgca cgctcggcta tgcctgctac ggcgagatag
     2281 cctgcacccg ccagcgctac gatctctact ggatgggggt ccatgccgac gcgcgaggca
     2341 agggtatcgg caaactgctc atgaaggaga ccgataaggc gatagcggcg cgcggcggtg
     2401 agatcgtcta tatcgagacc agttcacgcg agaaatatct gccgacccgc gagttctatc
     2461 gctccaccgg ctgtaaattg gaggccgaac tcaaggactt ctacgacaac ggcgacaaca
     2521 aggtcatcta tacccgccgc gtccgctaga cttttttctt ccgtaaccgc tcataggccg
     2581 cccgtaccgt atcggcgccg tactgacgct cgaggcggcg gatcgtgaag tgcgcctcgg
     2641 ccagttcctg atagtggtcc atgaagagcg tgttgagcac tgcgccactc gccgccccgg
     2701 cgaccgggac gacgcgggtc gccgcaagcc gcgtcgcgtt gacgccgaac ttcgccgcga
     2761 tcttcgagac gagtcccgaa aaggcgtgcc ccgccagttt gccgccggcg cccgaaagtt
     2821 cccgcgccgc ctgcgcgatc gtgccgtgca gggcgatacg tgcggcgtag taggtcgatc
     2881 cgaccgcctc gggggccgcg ccgatacccg aaagcgcgaa cacctcaaga catgcgagcc
     2941 gcgcggcggg gtcggagaga tcttccccgc tcttgcgcgc tatctcggcg atgcgccgga
     3001 gcatgagcac ggtggtgaga ggaagttccg ctatgagccc cggaagtccg gcggcgcctc
     3061 ccgtcgcccc cgagagggcg accatcgcct tgtggagatg gcgcgcgacc ggtccctccg
     3121 ggtcgctgcc gtttttgccg agcgtccgga ccgcgactga aagggcggcg gtgagcgccg
     3181 gcccgacgac cttttcggtg agccggacta tcgcggcggg aagattcttc gtcgttcggt
     3241 cggtcgcgcc gaaggtgagt tgcgagagtt ttgccgcgaa ggtgctttct tcgagcgtcg
     3301 cgaaggcctt tttaagttcg gtgcggtgcg cgggggagag ccgttttttt gatgccgccg
     3361 tcatcgggcg gtatccttga cgatgtcctg catggagtag tgtcccggcc ccttccggtc
     3421 gaggagccat gcgagagcac gaacggcccc ggcggcgaag atgtcgcggt cgtgggcgcg
     3481 gtgggtcagt tctatccgtt ctgcgtcgcc gaagaaggtg atggtatggt cgcccaccac
     3541 atcgccgccg cgcaacgcgt ggatggcgac atcgttcttt ccgcgacccg cgatcagtat
     3601 ctcgcctagt ttgagcgccg ttcccgaggg ggcgtccttc ttgcggctgt ggtgcgcctc
     3661 gacgatggcg acctccatac cggcggtgag ttgcgcgagg cgcggcagta tctcggcgat
     3721 gaccgcgaca ccgtaactca tgttggcgct gtggaagagc ggtatctttt tcgccgccgt
     3781 cgccatgagg gcgcggtcgg cgtcgatgag tcctgtcgtc ccggagagga acggtatacc
     3841 ggcggcgagc gcccggtcga gcgcttgacg gaaactttcg ggcgacccga agtcgaccac
     3901 cgcccggcac gaagcgagcg cggggtcggc gagtgtcgtc accgtgacgc ccgaggggcc
     3961 ggtgccgatg ctcgcgccgt ggtccttccc ttgcgccgga tggcccgacg cctctatcgg
     4021 cagaacggag aatccctcgt cggaaagccg ccgggtgatg gcgcggccca tgcggccgca
     4081 ggcgccgacc agagctatgc gtcgggtgtt gtcagtcgcc atagccgtat atctcctctt
     4141 tcaaactgcg ccccatcagt gcgctcatcg cggtgcgcac atcgagacct tcgtgcagga
     4201 cgcggtagac cgtttcgacg atgggcatcg tcacctccat cttctgggcg agttggtagg
     4261 ccgatatgct cgtcgcgacg ccctcggcga ccatcgtcat ccccgcgagg atgtcggaca
     4321 gtttctcgcc atgaccgatg cggatgccga cctgccggtt gcgcgacagt tcgccggtgc
     4381 aggtgagtat caggtcaccg agccccgaca gacccgatat ggtgaacggg ttggcgccta
     4441 tcttgaccgc gagtcgcgtg atctcggcta ttgcgcgggt catgagcgcc gactggctgt
     4501 tgcggcccag ccgcatcccc tcgacgatgc cgacgccgat ggcgatgaca ttctttatcg
     4561 cgccgccgac ctcgaccccg accacatcgg gtgaccggta gacgcggaag taggggagcg
     4621 aaaagaattc ctgagcggcg ttgagacctt cgatatagcg gcccgcgacg accgccgcgg
     4681 taggcatttt catgccgagt tcgcgggcga aggtcggccc cgaaaggacg aatatgttgc
     4741 ggtgcatgct ctcggggatg atctcctcgg ccacctgatg catgagtttg agcgtgccgt
     4801 tctcgatgcc tttcgacgcg atgaccagtt gactgccgtg caggaaccgg tacgaggcgc
     4861 ctgagaggat ggcgcgcgtg tgctgggccg gtgtcgccat gactatcatg tcgcggttgg
     4921 tcaccgccgc atcgagatcg ttggtcgcct ttaccttcgg gtggatgggt atgccgggca
     4981 ggaacaacga attctcgtgg tcgcggtcga tggcctcgac cagttcgggc tcgcggcacc
     5041 acagggagac ctcggcgttc ttctcggcca gtaccgaggc caggaccgtc ccccaactgc
     5101 cggcacctac cacgctgatg cgcatgttca tggtcgatcc tccatcatgt tccgccggat
     5161 ggcccgcagg gaacggggtc cagtgtaacg ggccgctttt ttttcgcaac taaaaatgtt
     5221 gcaaaaacgc tttttcccgc tatcatgacg ccgtaaccgt tttcgatatt ttcatgagga
     5281 ggcgtcatgg cgcgtgtcac ggtagaggat tgtctggaaa agataccgaa tcggctggaa
     5341 ctcatcatcc tgtcggccaa acggacgcgg cagttgctgg acaaggcggc cccgcaggtc
     5401 cccaacacta aaaagaacaa ggagccgatc gtggcgctcc gtgaagtggc cgccggcaag
     5461 atcgggctcg cccgggtcga ggcgaaaccg aaacgcccgc acttccgccg ccgtcgcatc
     5521 ggtaagtaca gcggcgcgca gaactgatgg cgctattccc cgggttcaat accaacatcc
     5581 gtcacaagaa taagatattc cacatacaga ccgaggtcat ctccaccggg gacaaacact
     5641 ccgtttcgac gctcgtatat cttgaggggc gcatattcca cgcggtcaag ggagtgcttc
     5701 cgccagccga ggcgatacac cgagagaccg ccgcgctcgt catcgtcaag cagcacaaag
     5761 aggcgataaa gaaactcata tccaaccaac tcgccgcagc cgactcggcg gcactgccgc
     5821 gcgggacgat aaacatcgtc gagttcacgg aattgtatca ag
//
LOCUS       DAOQUE010000124         5861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15032_length_5861_cov_3.594385, whole genome shotgun sequence.
ACCESSION   DAOQUE010000124 DAOQUE010000000
VERSION     DAOQUE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15032_length_5861_cov_3.594385"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKH10_05130"
     CDS             <1..201
                     /locus_tag="PKH10_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27549.1"
                     /translation="GRSPDRDLKDLVKKGYVKQIPREPYGGVYHYDETKRLVVTSSDN
                     LFKRADPDGEKEKAKKKETPKP"
     gene            224..799
                     /locus_tag="PKH10_05135"
     CDS             224..799
                     /locus_tag="PKH10_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNT27550.1"
                     /translation="MKGIKGTETEKNLLKSFAGESQARMRYDYFASAAKKEGYEKVAL
                     IFEETARQEKEHAKRFFKFLEGGDVEIVAAYPAGKIGTTAENLTGAMMGEHEEHTKLY
                     PAFAETAQKEGFPEIAAVWRNISNAEKHHEERYRTLLAEMEKGESFKKPAKTTWRCLN
                     CDFIHEGTEAPKNCPACAHPQAWFEELGKHW"
     gene            818..1186
                     /locus_tag="PKH10_05140"
     CDS             818..1186
                     /locus_tag="PKH10_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNT27551.1"
                     /translation="MTKRLEVYKCEICGNVVEMVHGAAGTLVCCGQNMKKMEEQTADW
                     KNEKHVPIAEAHEGATLVKVGSTAHPMVDDHYIEWIEIINGDYVNRKYLKPGDKPEAA
                     FYVPYADTLILREYCNKHGL"
     gene            1217..1726
                     /locus_tag="PKH10_05145"
     CDS             1217..1726
                     /locus_tag="PKH10_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNT27552.1"
                     /translation="MDKKLEKMINDQMNFEIYSAYIYLAMGAYFDAKGLTGFANWMKV
                     QWQEELFHAKKMYLYLLDKGGRPFYTAIPDPGKEWKSAKAAFEHALEHEKIVTSRINA
                     IKSAAMKLDDHATSVFYNWFVNEQVEEEKNVGEIIQKIEFVGEKGPGAYMLDKELAAR
                     VFVEPPLAE"
     gene            1739..2947
                     /locus_tag="PKH10_05150"
     CDS             1739..2947
                     /locus_tag="PKH10_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNT27553.1"
                     /translation="MTLSAIPIAPDIYWVGAIDWAVRNFHGYTTHRGTTYNAYLIIDK
                     KITLVDTVKKGFEHELLERIASVIDPSKIDYIISDHSEPDHSGTLREVVAAVKPEKVF
                     ASVVGQKTLARHFGDIGIPVTAVKDGYKLSLGKRTIAFMETRMLHWPDSMFSYLVEDQ
                     LLFSQDAFGMHLATAERFDDELSWDLLERMSSDYYANIITLYSPQVAKLLEKVAASGL
                     SFKMVAPDHGPIWRDMARFGKMVENYGKWSARELVRKAVILYDTMWHATEEMARYIAD
                     GIVAGGVKAEVMPLGSSSRSDVASMMIDAAALVVGSPTLNNNVYPSLADALTYLRGLK
                     FGTPVGAVFGSYGWSGEGTAQLKEYLASMNCAVIGEVKTQYVPDAGTLTACRDLGMQV
                     AAKVKELLPE"
     gene            2959..3117
                     /locus_tag="PKH10_05155"
     CDS             2959..3117
                     /locus_tag="PKH10_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNT27554.1"
                     /translation="MKKYVCTVCGYVYDPATGDPDSGVKPGTAWEDVPADWVCPVCGV
                     GKDSFEEQ"
     gene            complement(3121..3426)
                     /locus_tag="PKH10_05160"
     CDS             complement(3121..3426)
                     /locus_tag="PKH10_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27555.1"
                     /translation="MKKILFLIVAFVLFTGCAKEPSMVDVEKIIDEILGDRDSLLDDG
                     DTLLTEGDAAPADDDVLLTDGAPATDDVKKDADVAKDADALTADDLTPDDDTLLSDS"
     gene            complement(3438..4202)
                     /locus_tag="PKH10_05165"
     CDS             complement(3438..4202)
                     /locus_tag="PKH10_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27556.1"
                     /translation="MKRMMWIPCAALLFVATGVAAEPVPAAEPAPAAEPAPAAEPAPV
                     AEPAPAAEPAPAAEPAPAAEPAPAAEPAPAAEPAPGSVENSAVASPAVEEKKIDTAAT
                     EAPAKKEEPAAEGEKEIVRPYFGVAIASGVFGVLAIASGFYFDHLAQKSFTSYNDMTS
                     RSAIEEAAKSADFDRDAYVKKASQKYEEGRQHISSRNYSFIGGAIFLAAGVGLFFWTE
                     EKKPEEEEKKEEPVSDKKVSFVTAPDRFLVNFSASF"
     gene            complement(4211..5569)
                     /locus_tag="PKH10_05170"
     CDS             complement(4211..5569)
                     /locus_tag="PKH10_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT27557.1"
                     /translation="MFPLLIACDNEAKEWRLPEACQDRCAVVRGTDDLADAVANATDA
                     WNDCVCIDVATITTAVTVSKPLTIVGKGSSSRIVAPAGERGLLIRSDDVTLRDIEIAS
                     DTVALDIAQADGVALSGVRLVALPPAEGAVSFALAMKGGSITLTDTALTGSPDAATAA
                     GELAGSQTILNDVTMTGFGRQGLVVTGGAGLRWTGGGIADIRTENAALFVTGSSATLN
                     DIIIEKIAAPVTGSALYGGRGIVATDHAIIEATALTVRDCLSIGILIDGDAYGILDEA
                     VVTGNGMTGIWLQRSGDVPQLSLQDSTITGNRAAGIAAFRSCGLGIFDTRIAGTLSAQ
                     WHDALVGDGLTAYGVELDDADCVIRLERVVFADNFRAGFIADGAEGNAALAGLSFSGV
                     TVERSTIDAPGKYGIVIQNGTAPTGLSDGVTANPFDSADAALAAPLPIMTGTQDLFSV
                     YQ"
     gene            complement(5673..>5861)
                     /locus_tag="PKH10_05175"
     CDS             complement(5673..>5861)
                     /locus_tag="PKH10_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004685288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27558.1"
                     /translation="LIIALDRITLIDYKTGRVGHLAGKYREQMARYRAMLAGLYPGRS
                     VEAYLLFVDDPKRRVVAV"
BASE COUNT         1234 a         1788 c         1850 g          989 t
ORIGIN      
        1 ggccgttccc ccgatcgcga cctcaaggat ctcgtgaaga agggctatgt caagcagata
       61 ccgcgcgaac catacggcgg cgtctaccac tacgacgaga ccaagcggct ggtcgtcacc
      121 tcatcggaca acctgttcaa acgggccgat ccggacggtg aaaaagagaa ggcaaaaaag
      181 aaagagaccc cgaaacccta acatacggaa aaggaggatg cacatgaaag gtatcaaagg
      241 caccgagacc gaaaagaacc tgctcaaatc gttcgccggc gagtcgcagg cacggatgcg
      301 ttacgactat ttcgccagtg cggcgaagaa ggaaggttac gagaaagtgg ctctgatctt
      361 cgaggagacg gcgcggcagg agaaggagca cgcaaaacgg ttcttcaagt tccttgaagg
      421 cggggatgtc gagatcgtcg cggcctaccc ggccgggaag atcggcacga cggccgagaa
      481 cctcaccggc gccatgatgg gggagcacga ggaacacacg aaactctatc ctgccttcgc
      541 cgaaacggcc cagaaagaag ggttcccgga gatcgcggcc gtctggcgca acatctcgaa
      601 cgccgagaaa caccacgagg agcggtaccg cacgcttctg gccgagatgg agaagggcga
      661 gtccttcaag aagccggcta aaaccacttg gcgctgtctc aactgcgact tcatccacga
      721 agggaccgag gcgcccaaga actgcccggc atgcgcccat ccgcaggcat ggttcgagga
      781 actgggaaaa cactggtaac gataaggagg tgaggcgatg acaaaaagac tcgaggtgta
      841 caagtgcgag atatgcggca atgtggtcga gatggtccat ggcgcggccg ggacgctcgt
      901 ctgctgcggc cagaacatga agaagatgga agaacagacg gccgactgga agaacgagaa
      961 acatgtcccg atcgccgagg cgcatgaggg cgctaccctc gtcaaggtcg ggtcgaccgc
     1021 ccatccgatg gtcgacgacc actacatcga gtggatcgag atcattaacg gcgactatgt
     1081 gaaccgcaag tatctcaagc cgggcgacaa gcccgaggcg gcgttctatg tcccttacgc
     1141 cgacacgctg atactgcgcg aatactgcaa caaacacggc ctctgaagag gcggtaaaaa
     1201 gtaacggagg aacgccatgg acaaaaaact ggagaagatg atcaacgacc agatgaactt
     1261 cgagatatat tcggcctata tctacctcgc gatgggcgcc tacttcgacg cgaaggggct
     1321 gaccggcttc gcgaactgga tgaaggtgca gtggcaggag gaactgttcc acgcgaagaa
     1381 gatgtatctc tacctgcttg acaagggcgg acgccccttc tacaccgcga tccctgatcc
     1441 gggtaaggaa tggaagagcg cgaaggcggc cttcgaacac gccctcgagc acgagaagat
     1501 cgtcacctcc cgcatcaacg cgatcaaatc ggcggcgatg aaactggacg atcacgcgac
     1561 gagcgtcttc tacaactggt tcgtcaacga gcaggtcgag gaggagaaga atgtcggcga
     1621 gatcatccag aagatagagt tcgtcggcga gaagggcccc ggcgcctaca tgctcgacaa
     1681 ggaactcgcg gcgcgcgtgt tcgtcgagcc cccgctcgcc gaatagcggg gtggggtgat
     1741 gacgctctcc gcgataccga tagcgcccga catctattgg gtgggggcga tcgactgggc
     1801 ggtccgcaac ttccacggct acacgaccca tcgcggcacc acctacaacg cctatctcat
     1861 catcgacaag aagataacgc tcgtcgacac ggtgaaaaag ggcttcgaac acgaactgct
     1921 cgaacggatc gcatcggtga tagacccgtc gaagatcgac tatatcatct ccgatcactc
     1981 cgagccggac cactccggga cgctccgcga ggtggtcgcc gcggtgaaac ccgaaaaggt
     2041 cttcgcatcg gtcgtgggac agaagacgct cgcccggcac ttcggcgaca tcggcatccc
     2101 cgtcacggcg gtgaaggacg gctataaact gtcgctcggc aagcggacca tcgccttcat
     2161 ggagacgcgg atgctccact ggcccgacag catgttcagt tacctcgtcg aggatcaact
     2221 gctgttcagt caggatgcct tcgggatgca tctggcgacc gccgagcggt tcgacgacga
     2281 actgtcgtgg gacctgctcg aacggatgag ttctgattac tacgcaaaca tcatcacgct
     2341 ctactcgccg caggtggcca aactgctgga aaaggtcgcc gcctcgggac tttccttcaa
     2401 gatggtcgcc cccgaccacg gcccgatctg gcgcgacatg gcgcggttcg ggaagatggt
     2461 cgagaactac gggaagtggt cggcgcggga acttgtccgc aaggcggtca tcctctacga
     2521 cacgatgtgg cacgcgaccg aagagatggc gcgttacatc gccgacggca tcgtcgcggg
     2581 cggcgtaaag gccgaggtga tgccgctggg cagctcttcc cgcagcgatg tggcgagcat
     2641 gatgatagac gcggcggcgc tcgtcgtcgg gtcgccgacg ctcaacaaca atgtctatcc
     2701 ctcgctcgcc gatgcgctga cctacctgcg cggactcaag ttcggcacgc cggtcggcgc
     2761 cgtgttcggt tcctacggct ggagcggcga gggaacggcg caactgaaag agtatctcgc
     2821 ctcgatgaac tgcgcggtca tcggcgaggt gaagacacag tatgtccccg acgcggggac
     2881 gctcaccgcc tgtcgcgacc tcgggatgca ggtcgcggcc aaagtgaagg aacttttacc
     2941 cgaataatgg aggaacacat gaagaagtat gtctgcaccg tgtgcggtta tgtctatgat
     3001 ccggcgaccg gcgatcccga cagcggtgtc aagcccggca ccgcgtggga agatgtcccg
     3061 gccgattggg tgtgccccgt ctgcggggtc ggcaaggaca gtttcgagga acagtagggt
     3121 ctaactatcg ctgagcagcg tatcgtcgtc aggcgtcagg tcgtcggcgg taagcgcatc
     3181 ggcatccttt gccacatcgg cgtccttttt cacatcatcg gtggccggcg ccccatcggt
     3241 cagcaggaca tcatcgtccg ccggcgcggc gtcaccttcc gtgagcaggg tgtcgccgtc
     3301 atcgagcagg gaatcgcggt cgccgagtat ctcgtctatg atcttttcga catcgaccat
     3361 cgacggctcc ttggcacagc cggtgaacag cacgaaggcg acgatgagga acagtatctt
     3421 tttcatgacc gcctccccta gaatgacgcg ctgaagttga cgaggaaccg gtcgggcgcc
     3481 gtcacgaacg acaccttctt gtcggatacc ggctcctcct ttttctcttc ttcttcgggc
     3541 ttcttctcct cggtccagaa gaacaggccg acgcccgccg cgaggaatat cgcgccgccg
     3601 atgaacgaat agttgcgcga actgatgtgc tggcgcccct cctcgtactt ctggctcgct
     3661 ttcttgacat aggcgtcgcg gtcgaaatcg gcgcttttcg ccgcctcctc tatcgccgag
     3721 cgcgaggtca tgtcgttgta ggaggtgaaa ctcttctggg cgaggtggtc gaaatagaag
     3781 cccgaggcga tggcgagcac accgaagacg cccgatgcga tggcgacgcc gaaatagggt
     3841 cgcacgatct ccttctcgcc ttccgccgcc ggctcctcct tcttcgcggg cgcctcggtg
     3901 gcggccgtat ctatcttttt ttcttccacc gccgggcttg ccacggcgga gttctcaacg
     3961 gagccgggag cgggctcggc cgccggagcg ggctcggccg ccggggcggg ctcggccgcc
     4021 ggagcgggtt cagccgccgg agcgggctcg gccgccggag cgggctcggc caccggagct
     4081 ggttcagccg ccggagcggg ctcggccgcc ggagcaggtt cagccgccgg aacgggttcg
     4141 gccgcaacgc ccgtcgcgac gaaaagaagc gccgcgcacg ggatccacat cattcgtttc
     4201 atctcacacc tcactgatat acgctaaaaa ggtcctgtgt cccggtcatg atcggaagcg
     4261 gcgcggcgag cgcggcgtcg gcggaatcga agggattggc cgtcacgccg tcggaaagtc
     4321 ccgtgggcgc cgtcccgttc tgtatgacga tgccgtactt tcccggcgca tcgatggtcg
     4381 aacgttcgac cgtcacgccg gagaacgaca gcccggcaag cgccgcgttt ccttcggcgc
     4441 cgtcggcgat gaacccggcg cggaagttgt cggcaaagac gacccgctcc agccggatga
     4501 cacagtcggc gtcgtccagt tcgaccccgt aggcggtcag gccgtcgccg acaagggcgt
     4561 catgccactg tgccgagagc gtccccgcga tgcgggtatc gaatatcccg agaccgcagg
     4621 aacggaacgc cgcgataccc gcggcgcgat tccccgttat cgtggaatcc tgcagggaga
     4681 gttgcggaac atcgcccgac cgctggagcc agatgccggt catgccgttg ccggtcacga
     4741 ccgcctcgtc gagtatgccg tacgcgtcgc cgtcgatgag gatgccgatc gaaagacagt
     4801 cgcgcaccgt cagtgcggtc gcctcgatga tcgcatgatc ggtcgccacg atgccgcgac
     4861 cgccatagag cgcggaaccg gtcaccgggg cggctatctt ttcgataatg atgtcgttca
     4921 gcgtcgccga cgaaccggtc acgaagagcg ccgcgttctc ggtgcggata tcggcgatgc
     4981 cgccacccgt ccagcgcaac ccggccccgc cggtcacgac caacccctgc cgaccgaacc
     5041 cggtcatcgt gacatcgttg agaatcgtct gcgatcccgc cagctcgccg gcggcggtcg
     5101 ccgcgtcggg ggaaccggtc agcgcagtat cggtcagggt gatcgaacct cctttcatcg
     5161 caagggcgaa cgagacggcc ccttcggcgg gaggcaacgc aacgagccgc accccgctga
     5221 gcgcgacgcc gtcggcctgt gcgatatcga gcgcgacggt gtccgacgct atctcgatgt
     5281 ctcggagcgt cacatcgtcc gaccggatga gaagcccccg ctcccccgcc ggcgcgacga
     5341 tacggctcga cgaccctttc cccacgatgg taagtggttt cgagacggtg accgcggtcg
     5401 ttatcgtcgc gacatctatg cagacgcaat cgttccacgc gtcggtcgcg ttcgcgaccg
     5461 cgtcggcgag atcatcggtc ccgcggacga cggcgcagcg atcttggcac gcctcgggaa
     5521 gacgccattc ctttgcctcg ttatcgcagg cgataaggag cgggaacacg gccgcgcata
     5581 ggaaaataag tggtatcgtc gatggtcgca tgctcacctc gacgccatga tacccccgca
     5641 aaaagagaaa tcaaggaagt gtcgggacga attcagacgg cgaccacgcg ccgtttcggg
     5701 tcgtcgacga acagaaggta cgcctcgacc gaccggccgg ggtagagccc cgcgagcatg
     5761 gcgcggtacc gggccatctg ttcacgatac ttcccggcga ggtggccgac acggcccgtc
     5821 ttatagtcga tgagggtgat gcgatcgagc gcgatgatga g
//
LOCUS       DAOQUE010000125         5857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15049_length_5857_cov_4.122544, whole genome shotgun sequence.
ACCESSION   DAOQUE010000125 DAOQUE010000000
VERSION     DAOQUE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15049_length_5857_cov_4.122544"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PKH10_05180"
     CDS             <1..524
                     /locus_tag="PKH10_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27559.1"
                     /translation="QKAATLARLSTNTLGTSGAAINRTALYVKTDGFLVLTRGLSDSW
                     FGWNPATDTVLKKFDLPSRVTIAKVSNNQGVLSDINNPVSIAFTSTGQALVPNSGALA
                     PTNIPTFNCRKANNETIVYAARGTMFQVRVAVNSTNVINYELHIDDLGQHRICQAINS
                     ENFAGNGVEVLNI"
     gene            521..1072
                     /locus_tag="PKH10_05185"
     CDS             521..1072
                     /locus_tag="PKH10_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT27560.1"
                     /translation="MNGRGGFTLLEVMFSLLIFSAGMLAYVSYQARASAMLFETESTA
                     ISTAIAVEAAEELTSMTEDDFRILIETINPQPTTGWLTDTEFKAYSALFNFSIGPFDA
                     FGRALNGSGNGMFTRLFRVTTFSNLTNTAYEENSPMEVLRVIEIYVGWPNRGIVTKVC
                     DSGPGDAECNHLIVPIMKPIYYY"
     gene            1085..2113
                     /locus_tag="PKH10_05190"
     CDS             1085..2113
                     /locus_tag="PKH10_05190"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNT27561.1"
                     /translation="MVRRHGFTLIELMIAIVVSLFVIGTFLRFFSHAVRVEGRSTVKS
                     DITLKGNHILDTVESAIRLAGLGNSSLEFKNGAVILAASGVATNVDGGPLNSPASFTF
                     VSPWGGPVTKVAVIEGETPACRIYPQNNNAFTGDLNAVGTILLVNKANIFTAGYLRES
                     ATSIVTQTPFTYPDLSNYPNLLNVDAGLACAEMFPPHATILTGPNRAYTLTYDVTTQK
                     FRFFEAGATADIISMPREEIPFMVLQFLTEAEATGGEGELYTARLWTVAPADFSTIKA
                     VRFGFVMASRLPQDMNAALLTGIQYCMFGVCFTPPNSPDIQHKRYTAFSRVVYLRNID
                     SLKRHLNE"
     gene            2118..2717
                     /locus_tag="PKH10_05195"
     CDS             2118..2717
                     /locus_tag="PKH10_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27562.1"
                     /translation="MKKERTTIVSLFSRRRGMAVFIASLVLLAVAVLAMSTMTRISAT
                     SNLAGHTLAKKKLDLLADTALRVALGELTGQFIAASGSPSTMMVNGDVNNNNPALFRI
                     VADKRDGGNKPLFAYRAIARLLLIGDGNVTLPGMEFPVSNRNSCYDVELDTVEIVNIP
                     GTSVQTNIVQPTTGTLPTGYYYGQKKGLGVTTCFRKRNN"
     gene            2735..>5857
                     /locus_tag="PKH10_05200"
     CDS             2735..>5857
                     /locus_tag="PKH10_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27563.1"
                     /translation="MRPKILLIVMALVAVLAPLASRGAAQGESLKDDVFAMIATSEIL
                     NTMIVIDTSEQTNAFAYSGYIDTCNDAQSKLSKSISMCWQAYNDCAETNANLMCGASL
                     DCSSTLQKCNTLEANKVKLDNFCAMVQNEDRYGEPAVDEGVTDNAVCDPNVDPASKAC
                     RFIGPWTPNEMYDHDLCFYDWTADSGADIPNTDDYSYSQYGNCILVDPSDPTSGCKDM
                     KDLPDRRDWLCLSDDGSSVLASGLWLNWRFATSLDAIKILLADDHRFSYTPHRRGEVT
                     GVCRTRQWQPTGEGDQCWEYVNYATLSTEDKKTFQSMVRTQWVSTIKPGFEDNGKDYA
                     ACVGFAPVSTEGSIVSQPPTVPNAGCQQCFDRFGAATGCDEEKTYSFNNQPTDPPIVT
                     AIASNLRSDCRVFHCRTPRCRDDVETNCYAGLDPDPVNCPLGFYSEWDQDPEHCCDPV
                     GSGFDCVEEGGGGGGSYMPGPHKTTYVDREADFDNMEGVLTDDQFFSLAELEGGFSTD
                     VACVSDADCTVARVCNTGTNLCTGTSVACDTDDDCVFTLCDAATATCVGRSAWRVTDL
                     LPSENLLESVKVEMFYGCIGAMANTKFYEDIYTPSPTLVVWLPNAVPVSGCDTKGYKV
                     RIRLWVVHKGTGDGLASAQVDAQMKIAYIKADPSVLDVFNPAVIDYFEGDSWEQELED
                     PTSVVKEYECETTFYRMRSKVVSGGGGSCDTPQCDVAGGCSSYPSCVAKGNCTLPYPG
                     CTRKEIIAIGKDQWGSVTKSLCTWLCPDAPEYEDVWKCMGYFEANDTSPYPNGGTGPQ
                     GDTESTTYPPLDRVPCRPLENTVFECCRGLGAGATSFSSYYEILETFDTNTYKDIETG
                     SPTFGDSGNWKCGMAHFQEGIANNERRKTGGISVQITRGHINELPEGGTYLLNPILDS
                     FEWWGESGPKAPSPYKTSEDADGWYSAYTLPRAGSSAISASNISVFHTGENGTRDTAC
                     IYNIVQDLYGEDCDTCGMGCCMVDMGDIDYCSFPSFWIKVAKTDGGNLVLPLPAGHPL
                     RIDESPLTKDK"
BASE COUNT         1263 a         1744 c         1609 g         1241 t
ORIGIN      
        1 atcagaaggc tgcgaccctt gcacgactat ccacgaatac gctcggcacg agcggcgccg
       61 ccatcaaccg gacggcgctt tatgtcaaga ccgacgggtt cctcgtcctg acgcgaggac
      121 tcagcgattc ttggttcggg tggaatccgg cgaccgacac cgtacttaag aaattcgatc
      181 tgccgtctcg cgtgaccatt gccaaggtca gcaataacca aggggtcctg agcgatatca
      241 acaacccggt atccattgcc ttcacttcca cgggacaggc cttggtgccc aacagcggtg
      301 cgcttgcgcc gacaaatatc cccacattca actgccgaaa agcgaataac gaaaccatcg
      361 tgtacgcggc gagagggacc atgttccagg tgcgcgtggc cgttaactcg accaatgtca
      421 tcaactatga actacacatc gatgaccttg gtcagcatcg catctgtcag gccatcaatt
      481 ctgagaattt cgccggcaac ggagtggagg tgctgaacat atgaacggtc gcggcggttt
      541 cacgctactc gaggtcatgt tctccctgct catcttcagc gcgggcatgc tggcctatgt
      601 ttcgtatcag gcgcgcgcct cggcgatgct tttcgagacc gaatcgaccg cgatatcgac
      661 cgcgatcgcc gtggaagcgg ccgaggaact gacgagcatg accgaggatg actttcgcat
      721 cctcatcgag acgatcaacc ctcaaccgac gaccggctgg ctgaccgaca ccgagttcaa
      781 ggcctattcc gcgcttttca acttctccat aggcccgttc gacgccttcg gacgggcact
      841 caacgggagc ggcaacggca tgttcacccg cctgttccgc gtcaccacct tcagcaattt
      901 gaccaatacc gcctacgagg agaattcgcc catggaggtt ctccgcgtga tcgagatata
      961 tgtcggctgg cccaatcgcg gcatagtgac caaggtgtgc gattcgggtc ccggcgatgc
     1021 ggagtgcaac catctcatcg ttccgatcat gaagcccatt tattactact aggcagaggc
     1081 gataatggta cggcgacacg gtttcacgct catcgaactc atgatagcga tcgtcgtgtc
     1141 gctttttgtc atcggtacct tcctccgctt tttctcccat gcggtaagag tagagggacg
     1201 gagcaccgtg aagagcgaca tcacgctcaa gggcaatcat atcctcgaca ccgttgaatc
     1261 ggctatacgg ctcgcggggc tcggcaattc atcgctggag ttcaagaacg gcgcggtcat
     1321 cctcgcggct tccggcgttg caacgaatgt cgacggcggc ccccttaact cacccgcctc
     1381 cttcaccttc gtttcgccgt ggggcggccc ggtcaccaag gtcgccgtga tcgaagggga
     1441 aacgcccgcc tgtcgcatct atccgcagaa caacaacgcc tttaccggtg atctcaatgc
     1501 cgtcggcacc attcttctcg taaacaaggc gaacatattc accgccggat acctgcgcga
     1561 gagcgccacc tcgatcgtca cgcaaacacc gttcacttat cctgatctga gcaattatcc
     1621 caatctgttg aatgtcgatg ccgggcttgc ctgcgcggag atgttcccgc cgcatgctac
     1681 catcctgacc ggccccaacc gcgcctatac gcttacctac gatgtgacca ctcagaaatt
     1741 ccgattcttc gaggccggcg ccaccgcgga catcatctcg atgccgcgcg aggagatacc
     1801 gttcatggtc ctgcagttcc ttaccgaagc cgaggcgacc ggcggagagg gtgaactcta
     1861 caccgcccgc ctgtggaccg tcgcgccggc cgatttctcc acgatcaagg cggtccggtt
     1921 cggtttcgtc atggcctcgc gactcccaca ggacatgaat gccgcgttgc ttaccggtat
     1981 ccagtactgc atgttcggcg tttgtttcac gccaccgaac agtcccgaca tccagcataa
     2041 gcgctacacc gccttctcgc gggtggtata cctccgcaat atcgattctt tgaaaagaca
     2101 tctgaacgag tgatgatatg aagaaagaac gaacgaccat cgtttcgctg ttctcccgcc
     2161 gacgcggcat ggcggtcttt atcgcgtcgc tcgtcctgct cgccgttgcc gtcctcgcga
     2221 tgtccacgat gacccgcata tcggccacca gcaatctcgc gggccatacg ctcgccaaga
     2281 agaaactcga cctgcttgct gataccgccc tgcgggtcgc gctcggcgaa ctcaccggac
     2341 agttcatcgc cgcgagcggt tcaccctcga ccatgatggt gaacggagat gtgaacaaca
     2401 ataatccggc gctgttccgt atcgttgccg ataaacgcga cggcggcaac aagccgctgt
     2461 tcgcttatcg cgccatcgcg cggctcctgc tcattggtga cggcaatgtc accctgcccg
     2521 gcatggaatt cccggtctcc aaccgcaatt cctgttacga tgtcgaactg gacaccgtcg
     2581 agatagtgaa cattccgggt acctcggtcc agacgaacat cgttcagccg accaccggca
     2641 ccctgcccac cgggtattac tatggtcaga aaaaagggct cggggtgacc acctgcttca
     2701 gaaagcggaa taattgagga tgaaggagag atacatgagg ccgaagatct tactcattgt
     2761 catggcgctc gtcgcggtcc tcgccccact cgcctcgcgc ggcgcggcgc agggcgagag
     2821 tttaaaggac gatgtctttg cgatgatcgc cacctccgag atactgaaca ccatgatcgt
     2881 catcgatact tcggagcaga ccaatgcctt tgcctacagt ggatacatag acacctgcaa
     2941 tgatgctcaa tcgaaactca gtaaatcgat cagcatgtgc tggcaggcat acaatgattg
     3001 cgccgagacc aacgccaacc tgatgtgcgg tgcctcgctc gattgttctt cgaccctgca
     3061 gaaatgcaat acgcttgaag cgaacaaggt aaaactcgat aatttctgtg cgatggtcca
     3121 aaacgaggat cgttacgggg aacccgccgt ggacgagggg gtcacggaca atgcggtctg
     3181 cgatccgaat gttgaccccg ccagcaaggc gtgtcgcttc atcggcccgt ggacccctaa
     3241 tgagatgtac gatcacgacc tctgtttcta cgattggacc gcggacagcg gcgccgatat
     3301 tcccaacacc gatgactatt cttactctca atacggcaat tgcattctcg tggatcccag
     3361 cgatcccaca agcggttgca aagacatgaa agatcttccc gatcgacgcg actggctttg
     3421 cctttccgat gacggatcct cggtgcttgc ttcgggactt tggctcaact ggcgtttcgc
     3481 cacctcgctc gacgcgataa agatactgct cgccgacgac catcgtttct cttacacgcc
     3541 gcatcgtcgc ggcgaggtga ccggcgtctg ccggacccgg cagtggcaac cgaccggaga
     3601 aggggatcaa tgttgggagt atgtcaatta tgcgaccttg agcactgagg acaaaaaaac
     3661 cttccagtcc atggtgagaa cacagtgggt atcgaccata aaacccggat tcgaggataa
     3721 cgggaaagac tatgcggcgt gcgtcggttt cgccccggtt tccaccgaag gaagcatcgt
     3781 atcccagccc ccgaccgttc cgaacgccgg gtgtcaacag tgtttcgacc gtttcggtgc
     3841 cgctaccgga tgcgacgaag aaaagaccta ttccttcaac aatcaaccga ccgatccccc
     3901 catcgtgacc gcgatcgcgt cgaacctgcg gagcgattgt cgagtcttcc attgccggac
     3961 gcctcgatgt cgcgatgatg tcgagacgaa ttgctatgcc ggtcttgacc ctgaccccgt
     4021 caactgcccg ttaggttttt acagcgaatg ggatcaggat ccggagcatt gctgtgatcc
     4081 ggtcggcagt ggctttgact gtgttgaaga gggagggggc ggcggcggct cctacatgcc
     4141 cggtccccac aaaacgacct atgtggaccg ggaagccgat ttcgacaata tggagggggt
     4201 acttacggac gaccagttct tctctctcgc cgaactggaa ggaggatttt ccacggatgt
     4261 tgcctgtgtc agcgatgctg attgtaccgt tgcgcgcgtc tgcaatacgg gcacgaacct
     4321 ttgcaccggc acttcggtcg cttgtgatac cgacgatgac tgtgttttca ccctgtgcga
     4381 tgccgctacc gctacctgtg tcggtcgttc cgcttggcgc gtcacggatc ttcttccctc
     4441 ggaaaacctt ttggaaagtg tcaaggtcga gatgttctac gggtgcatcg gcgccatggc
     4501 gaacaccaag ttctatgagg acatctatac gccttccccc acgctcgtgg tgtggcttcc
     4561 caatgcggtc cccgtttccg ggtgcgacac caagggatac aaagtgcgca tccggttgtg
     4621 ggtcgttcac aagggtacgg gagacgggtt agcttcggcg caggtggatg cccaaatgaa
     4681 gatagcctac atcaaggcgg acccttcggt ccttgatgtt ttcaaccccg ccgttatcga
     4741 ttattttgaa ggtgattcct gggaacagga actcgaagat cctaccagtg tcgtaaaaga
     4801 gtacgagtgc gagaccacct tttaccgtat gcgctccaag gtggtgagtg gcggcggcgg
     4861 gagttgcgat acgccccagt gcgatgtcgc gggcggctgt tcctcttatc ccagttgtgt
     4921 cgcgaaaggc aactgtacct tgccgtaccc cggctgtacc cgcaaggaga tcatcgcgat
     4981 aggtaaggac cagtggggaa gcgtcaccaa gtcgctctgt acttggttgt gccccgatgc
     5041 gcccgagtac gaggatgtct ggaaatgcat ggggtatttc gaggcgaacg acaccagccc
     5101 ctatccgaat ggcggtaccg ggccgcaagg cgacacagag tcgaccacct accccccgct
     5161 ggatagagtg ccgtgccggc cgctcgagaa cacggtgttc gaatgttgtc gcggcctggg
     5221 ggccggggcg acgagttttt ccagttacta tgagatactc gagaccttcg acaccaacac
     5281 ctataaagac atagagaccg gttcgccgac ctttggagat tccggtaact ggaaatgcgg
     5341 catggcccac ttccaagagg gcatagcgaa caacgagcgc cgcaagaccg gcggcatcag
     5401 cgtgcagata acgagaggcc acatcaacga attgccggaa ggcggcacct atctactgaa
     5461 tcccattctt gattctttcg agtggtgggg agagtccggg cccaaggcac cgagtccgta
     5521 caagacctcg gaagacgccg atggctggta cagcgcctat actcttccgc gcgccggttc
     5581 ttcggccatc tccgcttcga atatctccgt ttttcacacc ggagagaacg gcacccgcga
     5641 caccgcctgt atctacaaca tcgtgcagga tctctacggt gaggactgcg acacctgtgg
     5701 catggggtgt tgcatggtgg atatgggaga catagattac tgtagtttcc cgagtttctg
     5761 gataaaggtc gccaagaccg acggcggcaa ccttgtcctg ccgcttccgg cggggcatcc
     5821 actccgaatc gatgagtctc ccttgacgaa agacaag
//
LOCUS       DAOQUE010000126         5852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15059_length_5852_cov_4.120062, whole genome shotgun sequence.
ACCESSION   DAOQUE010000126 DAOQUE010000000
VERSION     DAOQUE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15059_length_5852_cov_4.120062"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /locus_tag="PKH10_05205"
     CDS             <1..1880
                     /locus_tag="PKH10_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNT27564.1"
                     /translation="TSSKSSDRYLPLRLLAFCLSVGFACTRNTPATVDADILPKALND
                     HIRDYLEIVDSVGGRPLRDGRKLFLSRAGGEVRHLFVQEPDGSTRQLTRGPDAIDDYA
                     ASPDGSKILYLTSQGGNEQYDVHLLTVPDGATRPLLADPAVKTENPVWVDDRSFLYLS
                     NEVNKRDFYLYLYDLQTGTSRLLVEKKGVQAISDAMGADRILFHTILSNSSTVPYLLE
                     KGTARKLKGVQEDRLYRTIGFLRDGTMLIITDEGSDIEYLQIRDRGGKRSTVHAGPWP
                     VEQALIEKEGRDRAVFCTNEEGWSVCRLWHRGVIRELATGRGIVSLSDFTGGRLWYTL
                     SRPDAIPRPFVLDVAGGAPESFGITDARGVDIAAFVAPETRRAVSFDGLSVPYLLFLP
                     RGSTPPYRTITHYHGGPEGQARAGFNPMFQYYLKKGFAIAAPNVRGSTGYGRHYGALD
                     NYKLRMDAVSDAGAVMNDLVARGVSLPDRFIAMGGSYGGFMTVASMAQFPDRYRCGVD
                     VVGVVDFINFLKNTRSYRKALRETEYGPLEDEAFLHAISPTTMADRIKGKLFIAHGAN
                     DPRVPVSDAYILAEKLTAAGNKPEMLIFDDEGHGFRKKKNRIEFNERAASFINACAPL
                     Q"
     gene            2485..2793
                     /locus_tag="PKH10_05210"
     CDS             2485..2793
                     /locus_tag="PKH10_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT27565.1"
                     /translation="MAKKNTLIAKTKTDFLKVMKDGLAKHVALTNEQTKKVYDVFVQI
                     MKDTIASQNKLNLNGIGTFKVSNRKARMGRNPQTGKEIKIKASKTVGFRPTPSFKKEL
                     "
     gene            complement(2855..4480)
                     /locus_tag="PKH10_05215"
     CDS             complement(2855..4480)
                     /locus_tag="PKH10_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNT27566.1"
                     /translation="MARYLLPPVLFFCSYYGGAPLVIAMGVMALAAFLSRRSYAASVI
                     AAFLFATAGHVTAARDLLPMKETHEVDTADSEVIRLENGTVLRHDGTVIPRKGDEIAF
                     FDTAVVRDSGLPFLSSFEKVPERGLRKQLADLIRIRSDSTVLLALATGKRLFSADERA
                     AFLKTGTMHVVAISAFHIGLLFLLLHFVTRLLAFSPAFSPRGALLLSIAIKSLVLFWY
                     LSLTGWGAPTVRAALFLFLFDLFLVAGVPAHPFQMFLWSLIATAMILPRSMGSWSFIM
                     SALSVFTVIMVWRRASRSTLVSIVALTVAINFLLIPVSAELSGFLPLLAPLANLIVIP
                     FAGLMVLLLIPVQILFPFLPSVASFLLIPAEVTAHILTEDMRFLAALSDPTLVPLRQA
                     GVLWSALFYGLAAAVIFLRGWQRRVAFCALFLSVAPFVTAPFPENGVERIYSLPGNAY
                     CIREGRGVGRVVETQRYNFPAEYVQRKLDALPMSIERDVSRCGLMSVSSFHLRGPIPP
                     AARRDLAKRPRFSGASWHTVPEPSPLPPPEDRF"
     gene            4577..>5852
                     /locus_tag="PKH10_05220"
     CDS             4577..>5852
                     /locus_tag="PKH10_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27567.1"
                     /translation="MKKLLVVLLVLIAGAAFYLYDRTAGYREVRLCLVVSSDLGAGDR
                     AQTMARAVTAIRDLSRSCDGQTIVVDAGGLIGGDTAEWIVRTDPALPLPEAAFLTAAG
                     YRSVNLSPGYFRLGAWRILQLLQQSGVSAMTVNVAGGTPQRLEVDGISLTLYGLSLAP
                     DIAALPTGIFEGMTFQQPFGSSPFASGEQAGDLAILLLHSDSVSFAGASRMEPADLLS
                     SIHTQFPFLRLIFSDIPFPSRLSSDRSEGPRLISFTPGKPSLSVTDIRIIKHDGRSRG
                     IIAEMNTRSIDIARTPPDPQMRAVLSSFEEKVAAAAGQVVGTTLTGIDLARSRAGDNS
                     TLRLFHETLLAIAADAGHRADISIAIHPFSDMTRRPGAKIALSDVASMVPQDRFPVLI
                     KISGARLDDLLATATEAYPSDAVALFPFTYRIG"
BASE COUNT         1280 a         1840 c         1640 g         1092 t
ORIGIN      
        1 tgaccagttc gaagagttct gaccgatacc ttcccctacg gctcctcgcc ttctgccttt
       61 cggtggggtt cgcctgcacg cggaacaccc cggcgaccgt cgatgccgac atactcccga
      121 aggcgctgaa cgaccacata cgcgactatc ttgagatcgt cgattcggtc ggcggaagac
      181 cactgcgaga cgggaggaaa cttttccttt cccgcgccgg cggcgaggtg cgccacctgt
      241 tcgtgcagga gcccgacggc tcgacccgtc aactgacccg cggccccgac gcgatcgatg
      301 actacgcggc gagccccgac ggctcgaaga tcctctacct cacttcgcag ggcggcaatg
      361 aacagtacga cgtccatctg ctaacggtcc ccgacggcgc gacacggccg ctcctcgccg
      421 atccggcagt gaagaccgag aaccccgtct gggtcgacga ccgctccttc ctctaccttt
      481 cgaacgaggt caacaaacgc gacttctatc tgtaccttta cgatctccag accggcacga
      541 gcaggctgct cgtcgaaaaa aagggggtac aggccatcag cgacgcgatg ggcgccgacc
      601 ggatactttt ccataccata ttgagcaact cgtcgaccgt cccctatctg ctcgagaaag
      661 ggaccgcccg caaattgaaa ggcgtgcagg aggaccggct ctaccgtacg atcgggttcc
      721 tgcgcgacgg aacgatgctg atcatcaccg acgagggaag cgatatcgaa tacctgcaga
      781 tacgcgatcg cggcggcaaa cggagcacgg tccacgccgg cccctggccg gtggagcagg
      841 ccctcatcga aaaagaggga cgcgaccgcg ccgtcttctg caccaatgaa gagggatggt
      901 cggtctgccg cctctggcat cgcggtgtca tacgggaact ggcgaccgga cggggcatcg
      961 tgtcgctctc cgacttcacc ggcggccgcc tgtggtacac gctttcgcgc cccgacgcca
     1021 taccgcgtcc cttcgtgctc gatgtcgcgg gcggcgcacc cgaatcgttc ggcatcaccg
     1081 atgcgcgcgg tgtcgacatc gccgccttcg tcgcccccga aacgcgccgc gcggtctcct
     1141 tcgacggcct ttcggtgcca tatcttcttt tccttcccag gggatcgacg cctccgtacc
     1201 gcaccatcac gcactatcac ggcggccccg aaggacaggc acgcgccgga ttcaatccca
     1261 tgttccaata ctacctgaag aaaggtttcg ccatcgccgc gcccaatgtg cgcggctcca
     1321 ccggttacgg cagacactac ggcgcactcg acaactataa actgcgcatg gacgcggtca
     1381 gtgacgccgg cgcggtgatg aacgatctgg tcgcccgcgg tgtttcgctc cccgaccgct
     1441 tcatcgccat gggcggtagt tacggcggct tcatgacggt cgcttcgatg gcgcaattcc
     1501 ctgaccgcta ccggtgcggc gtggatgtgg tcggtgtggt cgatttcata aatttcctca
     1561 agaacacaag atcgtaccgc aaggcgctcc gcgagacgga gtacgggccc ctcgaggacg
     1621 aggcattcct ccacgcgatc agccccacca ccatggccga ccgaataaag ggaaaacttt
     1681 tcatcgccca cggcgccaac gatccgcgcg ttccagtgag cgatgcctat atcctcgctg
     1741 agaaactcac cgccgccggg aacaagccag agatgctcat attcgacgac gaaggacacg
     1801 ggttccggaa gaagaagaac cgcatcgaat tcaacgagcg cgccgcctcc ttcatcaatg
     1861 cgtgtgcgcc tcttcagtga actgaaagga cgagtacctt ttttctacgg atgcagacct
     1921 tcgaaagact tttttcattt ggcaaaaaaa gagtgcgtct tcaccaaaat atacattcct
     1981 cgctttgact agttcttatc tggtcttaca gaacgaaaaa gcctgttgga cacctgaagg
     2041 gaacggtcgg cattttccat agtgacatct ttttcataaa aaaaagcttt ttttctttca
     2101 tcgatcggat cggcgaaacg cctttattac gggtgttcta gaataacagg tctcgtcgcg
     2161 accctatttt tcaactccct ttttataccg acatcttata agagcagtaa tgacgcggcg
     2221 agaagaatgt ttaactcttg gaacccatat aataaaaggt cttcacgcat atcatatgcg
     2281 caacacattg taattactgc gtttttaatt ttcgaacttc tgttattgcg gaaatcaggt
     2341 gttgacaaaa acaataaaaa agaaaacaaa ctaccgaacc cccttatttt tttgattttt
     2401 ttaaagaaaa aggttgacaa atcttcctgt tctgctcata atgcttttgt cgtttagtaa
     2461 cctgagttat ggaggagaac atccatggcg aagaagaaca cgctgatcgc gaagacgaag
     2521 accgatttcc tcaaggtgat gaaggacggc cttgcgaagc atgtcgcgct gaccaacgag
     2581 cagaccaaga aagtgtacga tgtgttcgtt cagatcatga aggacaccat cgccagccag
     2641 aacaagctca accttaacgg catcggcacc ttcaaagtgt cgaaccgcaa ggcgcgcatg
     2701 ggccgcaacc cgcagacggg caaagagatc aagatcaagg cctccaagac cgtcggcttc
     2761 cgtcccaccc cgagcttcaa gaaggaactg taagaaagtc gttcctcgag aaccgttttt
     2821 cgccacctcc cggcaacggg aggttttttt ttacctagaa tcgatcctcg gggggcggga
     2881 gcggcgaggg ctccggcacc gtgtgccatg aggcgccgct gaaccgcgga cgcttggcga
     2941 gatccctgcg tgccgcggga ggtatcgggc cgcgcagatg gaacgaggag acgctcatca
     3001 gaccgcaccg actgacatcg cgttcgatcg acatcgggag cgcgtcgagt ttgcgctgga
     3061 catattcggc agggaaattg taccgctgcg tctccacgac gcgaccgacc cctcgtccct
     3121 cgcgaatgca gtaggcgtta cccggcaggc tgtagatgcg ttcgaccccg ttctcgggaa
     3181 aaggggccgt aacgaagggc gccaccgaaa gaaaaagcgc gcagaaagcg acgcgccgct
     3241 gccagccgcg aagaaagatg acggccgcgg ccagcccgta gaagagtgcg gaccagagta
     3301 ccccggcctg ccggagcggc accagcgtcg ggtcggacag cgcggcgagg aaacgcatat
     3361 cctcggtcag gatgtgcgcc gtcacctcgg cggggatcag gagaaatgat gcgaccgacg
     3421 gcaggaaggg gaacaggatc tgcacaggga tcaacagcaa caccatgaga cccgcgaacg
     3481 ggatgacgat gaggttcgcg agcggcgcaa gtaacggcag aaaacctgaa agttcggcag
     3541 agaccggtat caggaggaag tttatcgcca cggtcagtgc cactatggag acgagcgtcg
     3601 aacgggatgc cctgcgccac accatgatga ccgtgaacac cgaaagagcg ctcatgatga
     3661 aactccaact gcccatgctg cgcggcagta tcatcgcggt ggcgatgagc gaccacagga
     3721 acatctggaa cggatgcgcc ggcacgccgg ccacaaggaa aagatcgaac aggaacagga
     3781 acagcgcggc gcgcacggtc ggagcgcccc aaccggtcag ggaaaggtac cagaacagta
     3841 caagcgactt gatcgcgatg ctcaacagga gggcgcccct cggcgaaaac gcgggggaaa
     3901 aggccagcag acgcgtcacg aaatggagca ggaggaacaa gagaccgata tggaacgctg
     3961 atatcgcgac gacatgcatc gtaccggtct tgaggaacgc ggcccgctca tcggcggaaa
     4021 aaagccgctt gccggtcgca agcgccagca ggaccgtcga gtcgctccgg atacggataa
     4081 gatctgccag ctgcttgcga agaccgcgtt ccggcacctt ttcaaaggaa gagaggaacg
     4141 gcagtccact atcccgcacc accgcagtgt cgaagaaagc tatctcgtcc cccttccgag
     4201 ggatcactgt gccatcgtga cggaggaccg tgccattctc gagacggata acctcgctgt
     4261 cggccgtatc gacctcatgc gtttccttca tcggcagaag atcccgcgcc gccgtgacat
     4321 ggcccgccgt cgcgaacagg aaggccgcta tcaccgacgc cgcgtacgac ctccgggaca
     4381 ggaacgcggc aagcgccatg acgcccatgg cgatcacgag cggcgcaccg ccgtagtagg
     4441 aacagaagaa taggactggg gggagcaggt accgtgccat gcgcccatga taaaagaacc
     4501 gccgccattg tcaaaaagag tccgtctgga ctttttcgac tccgtcggta taccatgggg
     4561 acagaggagg agtcccgtga agaaacttct ggtcgtgctg cttgtcctta tcgccggagc
     4621 ggcgttctat ctttacgacc gtaccgccgg gtaccgggaa gtgcggctgt gtctcgtggt
     4681 gtcctcggac ctcggcgccg gtgaccgggc gcagaccatg gcacgcgccg tcacggcgat
     4741 acgggacctt tcccgctcct gcgacgggca gaccatcgtc gtcgacgccg gcggactcat
     4801 cggcggcgac accgccgaat ggatcgtgcg caccgatccg gccctccctc ttcctgaggc
     4861 cgcctttctt accgcggcgg gatatcgttc cgtgaacctt tcccccggct attttcgtct
     4921 cggcgcatgg cggatcctgc aactcctgca acagtcgggc gtctctgcga tgaccgtcaa
     4981 tgtcgccggc ggcacaccgc aacggctgga ggtcgacggc atctcgctga cgctgtacgg
     5041 cctttctctt gcccccgata tcgccgccct gcccaccggc atcttcgagg gcatgacctt
     5101 ccagcagccg ttcggctcct ctccgttcgc gtccggagag caggccggag accttgccat
     5161 ccttcttctc cactccgaca gcgtttcctt cgccggggcc tcgcgcatgg agccggccga
     5221 tcttctttcc tccattcata cccaattccc cttcttgcgc ctgatcttca gcgacattcc
     5281 cttcccctcc cggctttcgt cggacaggtc ggaaggtccc cgactgatat cctttacccc
     5341 cggaaagccc tctctctcgg tgaccgacat ccgcatcata aagcatgacg ggcgctctcg
     5401 cggcataatc gccgagatga acacccgctc catagacatc gcaagaacgc ctcccgaccc
     5461 gcagatgcgt gccgttctct cctcgttcga ggaaaaggtc gccgccgccg ccggacaggt
     5521 cgtcgggacg accctgaccg gtatcgacct tgcccggtca agagccggcg ataattcgac
     5581 cctgcgtctt ttccacgaaa cgcttctcgc catcgccgcc gatgccggtc atcgcgctga
     5641 catcagcatc gcgatccatc cattcagcga catgacgagg cggcccggcg caaagatcgc
     5701 tctttccgat gtcgcgagca tggtgccgca ggaccgcttc cccgtgctta tcaagatatc
     5761 cggcgcgcgt ctcgacgacc tccttgctac cgccacggag gcctaccctt ccgacgccgt
     5821 ggctcttttc cccttcacct accggatcgg ta
//
LOCUS       DAOQUE010000127         5851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15061_length_5851_cov_4.420807, whole genome shotgun sequence.
ACCESSION   DAOQUE010000127 DAOQUE010000000
VERSION     DAOQUE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15061_length_5851_cov_4.420807"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PKH10_05225"
     CDS             <1..942
                     /locus_tag="PKH10_05225"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNT27568.1"
                     /translation="RELFPPVYDTLWHGARSAFVTVMHGCDNHCSYCIVPSVRGGEVS
                     RTAETILEEIRALVASGIREVTLLGQNVNSYHDPAAGLDFTGLLYLIAGRSGLDRLRF
                     VTSHPRDLTPALAAAFAEIPSLMPYLHLPAQSGSDRILALMNRGYGVNEYQEKIAWVR
                     ERCPDISLSSDFIVGFPGETESEFEETLSLIGTVRYDTLFAFKYSPRPGTVAARWPET
                     LTDAERSARLDRLLHAQRAVMTAVRRRFLGRTVTVLIESRSPRGDTVMGRSEHNQIVH
                     VAGDPLPAEGSLVPVRITEVLDNTLRGDRIDGVKEDG"
     gene            939..1352
                     /locus_tag="PKH10_05230"
     CDS             939..1352
                     /locus_tag="PKH10_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNT27569.1"
                     /translation="MIRVRKTFSAHDFAIDVAPLVDVLFTLLIFFSLTSSFKQESGIK
                     VNLPEASASGAVEHIRRIEIVIPSAGAVRVGDRDMPVEEVAAHLGKIDTAERQGYIVV
                     IKGDAEASHGRVTTVLDTVRGLGFKNVAIATRSRR"
     gene            1349..1627
                     /locus_tag="PKH10_05235"
     CDS             1349..1627
                     /locus_tag="PKH10_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27570.1"
                     /translation="MNRWHLLVIGGLFLIVTGVVLVDIHRLRGEIARNREVIEKLTME
                     RSAAQQRMRELELRIERMKSDRQTREMTALRRYKMLRPDQYILHDAVD"
     gene            1611..3596
                     /locus_tag="PKH10_05240"
     CDS             1611..3596
                     /locus_tag="PKH10_05240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT27571.1"
                     /translation="MMRLIRSLPFLYAIPIVFIAISSSSRQSLSVQIVVWGLFVVLHL
                     YLALIERIALILPAFLPAVVYVADFVILRWGNHSPLIALLFAALPVVYARRPAHYGWY
                     VAASALSLALPGSLREARDLTVYVLFLLTAGAVYLFSREEIVRYLWGGGTARSGDHRS
                     PGASDHARQAVEDPYLALMKYCERTTLWDRIPVAVKVVELRGEEGIIVGAPSPFRLTG
                     LILLAIKNRCVLPCHSILEEKEFLPLIPPYNKRVYFPVGLFDPEGVPAVPEYVIVIDA
                     DYQGDKQLLVETFAPIRQDVIELLRMAATFDRVLTDRRRQEKLYRGAREMLDSFSREK
                     LFNASAWSVLNFVPDAATVLITEWRDGEHKGIIYRLEDDGTPLSLEKVAPLTATDMTD
                     PTTIQAMMLEGKVGKMAEIRDINRRRDKNQLFAELAFAELNRHAQMLTFLLQRHETPQ
                     GTLSVFLDSDREVDTQTKSDLLLLCRILSSALNNIEMYETVQNLSNIDALTGLYNRRY
                     LQERTETMTGEAARSGMPLSAIMLDIDHFKKVNDRYGHKAGDEVIRFLSRIIKRSVRK
                     VDVAARYGGEEFVLLLHNTTKEGALKVAEKIRETVQGSVVPADGNQLTITSSFGVSSF
                     PELVQRPEDLVKSADEALYESKEGGRNRVTLYMPKGS"
     gene            3610..4662
                     /locus_tag="PKH10_05245"
     CDS             3610..4662
                     /locus_tag="PKH10_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNT27572.1"
                     /translation="MKILVTGTAGFIGSHTARRLIDRGDTVVGLDSVNDYYDVTLKYG
                     RLAESGIAHDAVRPGETVQSAKFPNYRFIQLKLEERDALTALFEREKFDVVCHLAAQA
                     GVRYSLENPHAYIEANLVGFMNILENCRRYPVRHLVYASSSSVYGLNTAMPFSTSHAV
                     DHPVSLYAATKKANELMAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFLYTDAILAG
                     RPIEVFNHGNMQRDFTYIDDIVEGVVRVIDRPPAGDPSWDGARPDPASSHAPYRLYNI
                     GNSAPVKLTDFIEAIEQALGKKATRELLPLQPGDVPATYADVSDLTRDTGYRPATDIA
                     EGVRRFVAWYRAYYGR"
     gene            4635..>5851
                     /locus_tag="PKH10_05250"
     CDS             4635..>5851
                     /locus_tag="PKH10_05250"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT27573.1"
                     /translation="MVPRLLRPVTMLSREERSWVLYDAGNSAYSLIVVTVMFPLYFKG
                     TVASTLPPADATAYWGYANAGAALVLALISPLLGPLADMRGGKKPLFALFFLVGVVAT
                     ALLAIPLEGAWLWALGIFVLSRIGWGGSSFFYDSFLVDVSAPKRMHDVSSRGYAYGYI
                     GGTVPFLALVVWLFAVGGEGIPPHIAQAGFLLSALWWALFTVPMLRHVGQRYARPRSA
                     RPLRRSFEQLLRTLRTARRRRDAFLFLIAYFFYIDGVDTIIAMAAAYGVDAGLGAGTL
                     VGAILMIQIVAFPCAILYGRYATRYSARPLILTAIAVYLLITLIAFFLPDVAGPTGRA
                     ALFWVLSFLVATSMGGVQALSRSLFARLIPADRSAEFFGLFNIMGRFAAIMGPFLMGI
                     VASLAGHSRWGVVP"
BASE COUNT          982 a         1910 c         1842 g         1117 t
ORIGIN      
        1 cgtgagctgt tcccgccggt gtacgatacg ctgtggcacg gcgcccgttc ggccttcgtc
       61 acggtgatgc acgggtgcga caaccactgc agttactgca tcgtcccttc ggtgcgcggc
      121 ggcgaggtga gccggacggc cgagacgata ctggaagaga tccgcgcgct ggtcgcgtcc
      181 ggcatccgtg aagtgacgct tctcggccag aatgtcaaca gttaccacga ccccgccgcc
      241 ggtcttgact tcaccgggct gctgtacctt atcgctgggc ggtcggggct tgaccgcctc
      301 cggttcgtca cgagccaccc gcgcgacctc accccggcgc ttgccgcggc gttcgccgag
      361 ataccgtcgc tcatgcctta tctgcacctg ccggcccaat cgggatcgga ccggatactg
      421 gcgctcatga accgggggta cggcgtgaac gagtaccagg agaagatagc gtgggtccgc
      481 gagcggtgtc ctgacatctc gctgtccagt gacttcatcg tagggttccc cggagagacc
      541 gaaagcgaat tcgaagagac gctctccctt atcggaacgg tgcgctacga caccctgttc
      601 gccttcaaat actctccccg ccccgggacg gtcgcggcgc gatggcccga aaccttgacg
      661 gatgccgaga gatcggcgag acttgaccgg ctgttgcatg cccagcgcgc cgtgatgacc
      721 gcggtgcgac ggcggttcct cggcaggacc gtgacggtgc tgatagaatc gcgcagtccg
      781 cgcggcgaca ccgtcatggg gcggagcgag cacaaccaga tcgttcatgt ggccggcgat
      841 cccctgcccg ccgagggttc gctggtgccg gtgcgtatca ccgaggtgct cgataatacc
      901 ctgcggggcg acaggatcga cggcgtcaag gaggacggat gatacgggtt cgcaagacct
      961 tttcggcgca cgatttcgcc atcgatgtcg cgccgctcgt cgatgtcctg ttcacgctcc
     1021 tcatattctt ctcgctcacc agttccttca aacaggaatc ggggatcaag gtcaacctgc
     1081 cggaggcgag cgcgagcggc gcggtggagc atatccgccg catcgagatc gttatcccct
     1141 cggcgggcgc ggtccgtgtc ggtgaccgcg atatgcccgt cgaagaggtc gcggcgcatc
     1201 tgggaaaaat tgacaccgcg gagcggcagg gctacattgt cgtgataaaa ggggacgccg
     1261 aggcatcgca cgggcgggtg acgacggtgc tcgatacggt gcgaggactc ggtttcaaga
     1321 atgtcgccat agcgacaagg agcagacgat gaatcggtgg cacctgctag ttatcggcgg
     1381 gctgttcctg atcgtgaccg gagtggtgct ggtcgatatc catcgcctgc gaggcgagat
     1441 agcccgcaac cgcgaggtga tagagaaact gacgatggag cggtccgccg cgcagcaacg
     1501 gatgcgcgaa ctcgaactgc gcatcgagcg tatgaagagc gaccgccaga cccgcgagat
     1561 gacggccctg cggagataca agatgctgcg ccccgatcag tacatactcc atgatgcggt
     1621 tgattaggtc cctcccgttc ctgtatgcga tccctatcgt tttcatagcg atatcatcct
     1681 cttctcgtca gtcgctgtcg gtccagatag tcgtctgggg cctgttcgtg gtgctccacc
     1741 tgtacctcgc cctcatcgag cggatcgcgc tcatactgcc cgccttcctc ccggcggtgg
     1801 tctatgtggc cgacttcgtc atactgaggt ggggtaacca ttccccgctc atcgcgctcc
     1861 tgttcgcggc gcttccggta gtgtatgcgc ggcgtccggc ccactatggc tggtatgtcg
     1921 cggcctcggc cctttcgctc gccctgccgg gttcactgcg ggaggcgcgc gaccttaccg
     1981 tctatgtgct gttcctgctt accgcaggcg ccgtctatct gttcagccgg gaagagatcg
     2041 tccgctacct ctggggcggg ggcacggcgc gttccggcga tcaccggtcc cccggggcct
     2101 cggatcacgc gcggcaggcg gtcgaggacc cctatctcgc ccttatgaag tactgcgaac
     2161 ggacgacgct ctgggatcgc ataccggtcg ccgtgaaagt ggtggaactg cggggcgagg
     2221 agggcatcat cgtcggcgct ccgtcgccgt tccgcctgac ggggctgata ctgctcgcca
     2281 tcaagaaccg gtgcgtgctc ccgtgccatt cgatactgga ggaaaaggag ttcctgccgc
     2341 ttatcccgcc gtataacaag cgggtctatt tcccggtggg gctcttcgat ccggaaggag
     2401 tgcccgccgt ccccgaatat gtcattgtga tagacgccga ttatcagggc gataagcaac
     2461 tgctcgtcga gaccttcgcg ccgatcaggc aggatgtgat cgaactcctg cggatggcgg
     2521 cgaccttcga tcgggtgctc accgaccggc gccggcagga gaaactgtac cgcggcgcgc
     2581 gtgagatgct cgattcgttc agccgtgaga aactgttcaa cgcctcggcg tggtcggtgc
     2641 tcaacttcgt tcccgacgcc gcgaccgtgc tcatcaccga atggcgggac ggcgagcaca
     2701 agggcatcat ctaccggctc gaggacgacg ggacgccgct ttcgctcgag aaggtggcgc
     2761 cgctcaccgc taccgatatg accgatccca cgacgataca ggcgatgatg ctcgagggta
     2821 aggtcggcaa gatggccgag atccgcgaca tcaaccggcg ccgcgacaag aaccaactgt
     2881 tcgccgaact ggcattcgcg gaactgaatc ggcacgcgca gatgctcacc ttcctgctcc
     2941 aacgccacga gacgccgcag gggacgctct cggtgttcct cgactcggac cgcgaggtgg
     3001 acacccagac caaatcggac ctgctcctgc tctgtcgcat cctgtcgtcg gcgctcaaca
     3061 acatcgagat gtacgaaacg gtgcagaacc tttccaacat cgatgcgctg accggtcttt
     3121 acaaccggcg ttacctgcag gaacggaccg agaccatgac cggcgaggcg gcccgttccg
     3181 gcatgccgct gtcggccatc atgctcgaca tcgatcattt caagaaggtc aacgaccgct
     3241 acggccacaa ggcgggcgac gaggtgatac ggttcctgtc gcgcatcatc aagcggtcgg
     3301 tccgcaaggt cgatgtcgcg gcgcgctacg gcggcgagga gttcgtactt cttctgcaca
     3361 acaccaccaa agagggtgcg cttaaggtcg ccgagaagat acgcgagacg gtgcagggtt
     3421 cggtcgtccc ggccgacggc aaccaactca ccatcacctc gagtttcggc gtctcgtcgt
     3481 tccccgaact ggtccagcgc cccgaggacc tcgtcaagtc ggccgacgag gcgctgtacg
     3541 agtcgaaaga gggggggcgc aaccgcgtga cgctctatat gccgaaggga tcgtgagcgg
     3601 gaggtcagca tgaagatact cgtcaccggc accgccgggt tcatcgggtc ccacaccgcc
     3661 cggcggctta tcgaccgcgg cgacacggtg gtgggactcg acagcgtcaa cgactactac
     3721 gatgtgacgc tcaagtacgg gcggctcgcc gagagcggca tcgcgcacga cgcggtgaga
     3781 cccggcgaga ccgtgcagag cgcgaagttc ccgaactatc gcttcatcca gttgaaactt
     3841 gaagagcgcg atgccctgac cgccctgttc gagcgcgaga agttcgatgt cgtctgccac
     3901 cttgcggcgc aggcgggggt ccgctattcg ctggagaacc cgcacgccta catcgaggcc
     3961 aacctcgtcg gcttcatgaa catcctcgag aactgccgcc gctatccggt gcggcatctc
     4021 gtctatgcct ccagttcgag cgtctatggg ctcaacaccg cgatgccctt ctcgacctcg
     4081 cacgcggtgg accacccggt gagtctctac gcggcgacca agaaggcgaa cgaactgatg
     4141 gcgcatacct acagccacct gtacggtctg ccgacgaccg gcctgcgctt cttcaccgtc
     4201 tacggtccgt ggggacggcc cgacatggcg ctgttcctct acacagacgc gatactcgcg
     4261 gggcggccga tcgaggtctt caaccacggg aacatgcaac gcgacttcac ctacatcgac
     4321 gacatcgtcg aaggggtggt gcgggtcatc gaccggccgc ccgccggcga tccgtcatgg
     4381 gacggtgcgc ggcccgaccc ggcctcgtcg cacgccccgt accgtctcta caacatcggc
     4441 aacagcgcgc cggtgaaact gaccgacttc atcgaagcga tagagcaggc gctcggcaaa
     4501 aaggcgaccc gtgaactgtt gccgctccag ccgggcgatg tccccgccac ctacgccgat
     4561 gtgtccgatc tcacgcgtga caccgggtac cgacccgcca ccgacatcgc ggaaggtgtc
     4621 cggcggttcg tcgcgtggta ccgcgcctac tacggccggt gacgatgctt tcccgcgagg
     4681 agcgcagttg ggtcctctat gatgcgggga actccgccta ttcgctcatc gtcgtcacgg
     4741 tgatgttccc gctctatttc aaaggcaccg tcgcctcgac gcttccgccc gccgacgcga
     4801 ccgcctactg gggctacgcg aacgcggggg ccgcgcttgt cctcgcgctc atctcgccgc
     4861 ttctcgggcc gcttgcggat atgcggggcg gcaagaagcc gctgttcgcg ctgttcttcc
     4921 tcgtcggcgt cgtagccacg gcgttgctcg cgatcccgct ggagggcgcg tggctctggg
     4981 cgctcggcat cttcgtgctg tcgcgcatcg gttggggcgg ctcgtcgttc ttttacgatt
     5041 cgttcctcgt tgatgtgtcc gcgccgaagc ggatgcacga tgtctcctcg cggggctatg
     5101 cgtacggcta catcggcggt accgtgccgt tcctcgcgct cgtcgtctgg ctgttcgctg
     5161 tgggcgggga gggtatccca ccccacatcg cgcaggccgg gttcctcctg tcggcgctct
     5221 ggtgggcgct cttcactgtt ccgatgcttc gccatgtcgg ccagcgctat gcgcggcccc
     5281 gctcggcgcg accgctccgt cgcagtttcg aacaactcct ccggaccctc cggaccgccc
     5341 ggcggcggcg cgacgcgttc ctgttcctta tcgcctattt cttctacatc gacggcgtgg
     5401 acaccatcat cgcgatggcg gccgcctacg gggtggatgc ggggctcggc gccggcacgc
     5461 tcgtcggagc gatactgatg atccagatcg tcgcgttccc ctgcgccata ctgtacggcc
     5521 ggtacgcgac ccgttacagc gcccgaccgc tcattctgac agcgatagcg gtctatctcc
     5581 tcatcacgct catcgccttc tttctccccg atgtggcggg tccgacgggg cgcgccgccc
     5641 tcttctgggt gctttcgttc ctcgttgcca cctcgatggg gggggtgcaa gcactcagcc
     5701 ggtcgctctt cgcgcggctc atccccgccg accgatcggc agagttcttc ggtcttttca
     5761 acatcatggg gcggttcgcc gccatcatgg gcccgttcct gatggggatc gtcgccagtc
     5821 tcgcggggca ttcgcgctgg ggggtcgtcc c
//
LOCUS       DAOQUE010000128         5827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15153_length_5827_cov_4.150728, whole genome shotgun sequence.
ACCESSION   DAOQUE010000128 DAOQUE010000000
VERSION     DAOQUE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15153_length_5827_cov_4.150728"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PKH10_05255"
     CDS             complement(<1..289)
                     /locus_tag="PKH10_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27574.1"
                     /translation="MKRKELVLLMFAGAILFVLGACGSPESPYAPDDVIVLPDNDGNT
                     LPDSDKLVNDDGQLVNDDGQLINDDGQVINDDGQVIGDGDTPVGDSDTPVGD"
     gene            398..895
                     /locus_tag="PKH10_05260"
     CDS             398..895
                     /locus_tag="PKH10_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27575.1"
                     /translation="MEPRKERRFPDMSMHGVPPRSLSLPLVLAALAALVFVGWLAWRW
                     YNGVTPDKAVYEMMPTGKGSEEAAPKEPPPPEAPEPPVKVEIKSLAPAPATEKGLYTE
                     KEIERLQKKIETPSKKKNTYTNADLQKYRARPGTPDTQKVKKEIEWAEEPEGGDATRR
                     QNPSH"
     gene            complement(867..1325)
                     /gene="ndk"
                     /locus_tag="PKH10_05265"
     CDS             complement(867..1325)
                     /gene="ndk"
                     /locus_tag="PKH10_05265"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNT27576.1"
                     /translation="MQRERTLVLIKPGAIQRELIGEIITRFERKGIKIVGLKMLRMTE
                     KLVNEHYAHLREQPFFRDIKLSMMETPIVAIALEGYDIVRRVRTLSGATNPREAAPGT
                     IRGDYSISIRNNVVHASATIEEALDELQRFFEEGEVFSYQRINVMDFADE"
     gene            complement(1349..2407)
                     /locus_tag="PKH10_05270"
     CDS             complement(1349..2407)
                     /locus_tag="PKH10_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007420597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT27577.1"
                     /translation="MKTFRFALIGCGRIAKKHYEAIAAMPNASIAAVCDIVPERARST
                     GEKLGVPWYTDYHEMLEKETVDIVNILTPSGLHAEHTIDVVTRYGKEIVCEKPMALRL
                     PDADRMIRACDAKRVHLFVVKQNRYNLPVQKLREALEAGRFGRIALGTVRVRWSRDQR
                     YYDQDAWRGTWAMDGGVVVNQASHHIDLLLWMLGEPVSVMAKSGTYFSNIEVDDTALA
                     IVTFRSGALGLIEATTATRPDDLEGSLSVLGERGSVVIGGFAVNRMDTWRFVDEAPEE
                     SAAVVKEFSEMPPNVYGYGHQRFLEHVVDCLEHGKHALIDGIEGRKTIELFNAIYESV
                     ETGREVALHFEPRLGRRS"
     gene            complement(2404..3522)
                     /locus_tag="PKH10_05275"
     CDS             complement(2404..3522)
                     /locus_tag="PKH10_05275"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF024684.2"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNT27578.1"
                     /translation="MSARTLLVYGITGSIGTSTLKVVEAFPDRFSIIGCSAGSDRAAL
                     DAMTPRLPALRRSVCSTDAKELLTLLDLSPDLVVMALGGKSGWKITLEALRRGIPVAL
                     ANKESLVIAGYFLGRATTGDRTKVIPIDSEHAALMQLLRAVRPDHIRRVHLTASGGAL
                     LELSKKAMLDADAATALKHPVWSMGKKVTVDSATMVNKGLELMEAYWLFPVEPEQLGV
                     IVHPEVAVHATVDLIDGTSLWQAAVSDMAIPIAAALAYPDLLPMLDRFPRFAYPHDRA
                     LAFRAPDLDKYPLLALAMDLLRRKDYAGMVAYAIADETAVEAFLAGRIAVRGIHRTVE
                     RSVERFRGRSAPLDISALEDFIAEIETDAIRTVRETTP"
     gene            complement(3512..4327)
                     /locus_tag="PKH10_05280"
     CDS             complement(3512..4327)
                     /locus_tag="PKH10_05280"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNT27579.1"
                     /translation="MKHLKRYLTAVVAIPLAILLVWAAPAPLFLGVTMVFAYIAYSEF
                     VVMHKSRSAIVFLPAAALLMFAIYAYATAWHLFLLHIAAAFILIPLFALLGSDPIPDK
                     HQRLMIHLAGFLYLVIPFSLFSALRGYPDPTDGRKWFLFALVMPWVCDSAAFFVGSAI
                     GKHRLAPQISPNKSWEGAIAGLIASVAAGAAFSYIAFDGRYLIFSLVTAALASTAGQA
                     GDLVESLFKRGAGVKDSGTLFPGHGGILDRLDSVLFSVTVTYIALKYMEYHVG"
     gene            complement(4324..5181)
                     /locus_tag="PKH10_05285"
     CDS             complement(4324..5181)
                     /locus_tag="PKH10_05285"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNT27580.1"
                     /translation="MTFQELVFRLQRFYADRGCVLMQPYDLEMGAGTFHPATFLYALD
                     PRCINTVYVQPSRRPTDGRYGESPNRMQRFYQLQVLIKPSPADAQGLYLDSLRAIGIE
                     PKDHDIRFVEDDWESPTLGAWGLGWEVWLDGMEISQFTYFQQIGGLDLKPVPLELTYG
                     LERICMYLQEVDNIYDLKWNDTVRYGDIHKQDEVEFSTFNFKEAPVEDMFRFFDTYER
                     ISAELLAKGLPRPAYEFCIKASHAFNLLDARGAISVTERQRYIARIRTLACAAAQGYL
                     NFFASEEKK"
     gene            5360..>5827
                     /locus_tag="PKH10_05290"
     CDS             5360..>5827
                     /locus_tag="PKH10_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27581.1"
                     /translation="MNATNFLSVRGLLPLCLWLLWAPCVAAADSAESIVVWRVEPKTG
                     VTEKDADVVSAMVAMEVGRISGRQVIGETEMRSLMVGEEKKLSCGADDTACYAEIGAA
                     LGAPESIIGTLAKMGDYWILYLQRLNVRTASVLGRAEFKAKGDINLLVEGIPPA"
BASE COUNT         1122 a         1707 c         1941 g         1057 t
ORIGIN      
        1 tgtcaccgac cggagtatcg ctgtcaccga ccggggtatc gccgtcaccg atgacctgac
       61 cgtcgtcgtt gatgacctga ccgtcgtcgt ttatcaattg accgtcgtcg ttgacgagtt
      121 gaccgtcatc gttgacgagt ttatcgctgt ccggcaaggt attcccgtca ttgtcgggaa
      181 ggacgatgac atcatccgga gcgtaggggc tctcggggga gccgcaggca ccgaggacga
      241 aaaggatcgc gccagcgaac atcaggagaa caagttcttt tcttttcatg aggacaaccc
      301 cattttttcc gtggtggttc aacatgctag tcattgtaag aaaaacgcca ctgaagtcaa
      361 gcataaaaac ttgcgtggag aggggaggcg ctgtacgatg gaaccccgta aagaacggag
      421 gttccccgat atgtccatgc atggcgtccc gccgcgttcc ctgagtttgc cgctcgtcct
      481 cgcggcgttg gcggcgctgg tgttcgtcgg ctggctcgcg tggcggtggt ataacggcgt
      541 gacgcccgat aaggcggtgt acgagatgat gccgaccgga aagggatcgg aagaggcggc
      601 gccgaaagag ccgccgccac cggaggcgcc ggagccgccc gtcaaggtcg agataaaaag
      661 tctcgcgccg gccccggcga ccgaaaaggg cctgtacacc gaaaaggaga tagagcgcct
      721 gcagaaaaag atagagacgc cgtcgaagaa gaagaacacc tacaccaacg ccgacctgca
      781 gaaataccgc gctcgtcccg ggacgcccga tacgcagaag gtcaaaaaag agatcgagtg
      841 ggccgaagag ccggagggcg gggacgctac tcgtcggcaa aatccatcac attgatgcgc
      901 tggtaggaga atacctctcc ctcctcgaag aaccgttgca gttcgtcgag cgcctcctcg
      961 atggtggccg aggcgtgcac gacattgttg cggatggata tcgaatagtc gccccgtatc
     1021 gtgccgggtg ccgcttcgcg cgggttggtg gcgccgctca gggtgcggac gcgccgtacg
     1081 atgtcgtacc cttcgagcgc tatcgcgacg atgggggtct ccatcatgga aagttttatg
     1141 tcccggaaga acggctgttc gcgaaggtgc gcgtagtgtt cgttgacgag tttctcggtc
     1201 atgcgcagca tcttgaggcc gacgatcttg atccccttgc gttcgaaccg cgtgatgatc
     1261 tcgccgatga gttcgcgctg tatcgcgccc ggcttgatga ggaccaaggt ccgttcccgc
     1321 tgcatgacga cacctccgtg agtggttgtt aggatcttct gccgagccgt ggttcgaagt
     1381 ggagcgccac ctcgcgtccc gtctcgaccg actcgtatat cgcgttgaac agttctatgg
     1441 tcttgcgccc ctcgatgccg tcgatgagcg cgtgtttccc gtgctcgaga cagtccacga
     1501 catgttcgag gaaccgctgg tggccgtacc cgtacacatt cggcggcatc tcggagaact
     1561 ccttcacgac cgccgcgctc tcctcgggcg cctcgtcgac gaaccgccaa gtgtccatcc
     1621 ggttgacggc aaagccgccg atgacgaccg acccccgttc gccgagcacc gacagcgacc
     1681 cctcgagatc gtcggggcgc gtcgcggtgg tcgcctcgat gagtccgagc gcgccggaac
     1741 ggaaggtgac gatggcgagc gccgtgtcat ccacctcgat gttggagaaa taggtgccgc
     1801 tcttggccat gaccgatacc ggctcgccga gcatccagag caggaggtct atgtggtggg
     1861 aggcctggtt gacgacgacc ccgccgtcca ttgcccaggt gccgcgccat gcgtcctgat
     1921 cgtagtaccg ctggtcgcgg gaccaccgga cccgcacggt gccgagcgcg atgcgaccga
     1981 accgccccgc ctcaagcgcc tcgcgcagtt tctgcaccgg caggttgtag cggttctgct
     2041 tcacgacgaa caggtggacc cgtttcgcgt cgcaggcccg tatcatccgg tcggcgtcgg
     2101 ggagccgcag ggccatcggt ttctcgcaga cgatctcttt accgtaccgc gtgaccacat
     2161 cgatggtgtg ttcggcgtgc agaccgctcg gggtcagtat gttcacgatg tcaacggtct
     2221 ccttttcaag catctcgtgg tagtcggtgt accacggcac ccccagtttc tcgccggttg
     2281 accgcgcccg ctccggcacg atgtcgcaga cggcggctat ggaggcgttc ggcatggcgg
     2341 ctatcgcctc gtaatgcttc ttcgcgatgc gaccgcaccc gatgagggcg aaccggaagg
     2401 tcttcatggc gtcgtctccc gtaccgttcg aatggcatcc gtttctatct ctgcaatgaa
     2461 atcctcgagc gcggaaatgt caagaggagc cgaccgaccg cggaaccgtt cgacgctccg
     2521 ctcgacggtc cggtggatgc cgcgtaccgc gatgcggccc gcgaggaacg cctcgaccgc
     2581 cgtctcgtcg gcgatggcat aggcgaccat cccggcgtaa tctttgcgcc gcaggaggtc
     2641 catggcgagc gcgagaagag ggtatttgtc gagatccggc gcccggaagg cgagcgcccg
     2701 gtcgtgcgga taggcgaaac gggggaaccg gtcgagcatc ggcaggagat cgggataggc
     2761 gagcgccgcg gctatcggta tcgccatgtc gcttaccgcc gcctgccaga gactcgtgcc
     2821 gtcgatgaga tcgaccgtgg cgtgcaccgc cacctcgggg tggacgatga caccgagttg
     2881 ctccggctcg accgggaaga gccagtaggc ctccatcagt tcgagcccct tgttgaccat
     2941 cgtggccgaa tcgaccgtga ccttcttgcc catggaccag acggggtgtt tgagcgccgt
     3001 cgcggcgtcg gcgtcgagca tcgccttttt tgaaagttcc agcagggcgc cgcccgacgc
     3061 ggtgagatgg acccgccgga tatggtcggg ccgaacggcg cgcaggagtt gcatgagcgc
     3121 cgcgtgctcc gaatctatcg ggatgacctt cgtccggtcg ccggtcgttg cgcgaccgag
     3181 aaaatagcct gcgatgacga gcgattcctt gttggcgagc gcgaccggta tgccgcgtcg
     3241 cagggcctcc agcgttatct tccagcccga cttgccgccg agcgccatga cgacgagatc
     3301 gggggaaaga tcgagcaggg tcaagagttc cttcgcatcg gtggagcaga ccgaacgcct
     3361 gagggcgggg agccgggggg tcatcgcatc gagcgccgcg cggtcgctgc cggccgaaca
     3421 gccgatgatg gagaaccggt cgggaaaggc ctcgaccacc ttgagcgtgc tggtgccgat
     3481 ggagccggtg atgccgtaga cgagaagcgt tctagccgac atggtactcc atgtacttga
     3541 gggcgatata ggtcacggtg acgctgaaga gcaccgaatc gagccggtcg agtatgccgc
     3601 cgtgccccgg gaaaagcgtg cccgaatcct tgacgccggc gccccgtttg aacagcgact
     3661 cgaccagatc gcccgcctgc ccggcggtcg aggcgagcgc ggcggtcacg agcgagaaga
     3721 tgagatatct gccgtcgaag gcgatatacg agaacgcggc cccggcggcg accgacgcga
     3781 tgagcccggc gattgcgccc tcccaactct tattggggct tatctggggc gccagccggt
     3841 gtttgccgat cgcgctcccg acaaagaagg cggcgctgtc gcagacccac ggcatgacga
     3901 gcgcgaacag gaaccatttt ctgccgtcgg tcgggtcggg gtatccccgg agcgccgaaa
     3961 agagcgagaa ggggatgacg aggtagagga acccggcaag atggatcatc aggcgttggt
     4021 gtttgtccgg tatcggatcg gagccgagaa gcgcgaacag cgggataagg atgaatgcgg
     4081 cggcgatgtg gagaaggaac aggtgccatg ccgtcgcgta ggcatagatg gcgaacatga
     4141 ggagcgccgc cgcaggaagg aacacgatag cgctgcggct cttgtgcatc acgacgaact
     4201 cggagtaggc gatataggcg aacaccatcg tcacgccgag aaagagcggc gccggggcgg
     4261 cccagacgag cagtatcgcg agcgggatgg cgacgaccgc cgtgagatag cgcttgaggt
     4321 gcttcatttc ttttcctccg atgcgaagaa gttgagatag ccctgcgcgg cggcgcaggc
     4381 gagtgtccgg atgcgggcga tgtagcgctg gcgttcggtg accgatatgg cgccgcgcgc
     4441 gtcgagcagg ttgaaggcgt ggctcgcctt gatgcagaac tcgtaggcgg gacgcggcag
     4501 gcccttggcg agcagttcgg cgctgatgcg ttcgtaggtg tcgaagaagc ggaacatatc
     4561 ctcgaccggc gcctccttga agttgaaggt cgagaactcc acctcgtcct gcttgtggat
     4621 atcgccgtac ctcaccgtgt cgttccactt gagatcgtag atgttgtcga cctcctgcag
     4681 gtacatgcag atgcgttcga gcccgtaggt gagttccagc ggcaccggct tcaggtccag
     4741 cccgccgatc tgttggaagt aggtgaactg gctgatctcc atcccgtcga gccacacctc
     4801 ccagccgagt ccccacgcgc cgagcgtcgg gctctcccag tcgtcctcga caaagcggat
     4861 gtcatggtcc ttcggctcga tgccgatggc ccgaagactg tcgagataca gcccctgcgc
     4921 gtcggcgggg ctgggcttga tgagcacctg caactggtag aaccgctgca tccggttcgg
     4981 gctctcgccg taacgaccgt cggtggggcg gcgcgagggc tggacataga cggtgttgat
     5041 gcaccgcgga tcgagcgcgt agaggaaggt ggcgggatgg aaggtgccgg cgcccatctc
     5101 gaggtcgtag ggttgcatca agacacagcc gcggtcggcg tagaaccgct ggagccggaa
     5161 gacgagttcc tgaaaggtca gtttttccat cgggtacctc ttcgcgcagt ggcgcggcca
     5221 tactaacaca aagacggcgc aagtcaaaaa gaaagaagaa gccgaaaaaa ttgaaaaaaa
     5281 agcgctcgca cggtactctg tgccgcaacg ggcgtggagc gatgtcagag taacttaccc
     5341 accgggagat agcgacgata tgaacgcgac gaacttcttg agcgtgcggg gcctcctgcc
     5401 tctgtgtctc tggcttcttt gggccccctg tgtggcggcc gccgatagtg ccgaatccat
     5461 cgtggtctgg cgcgtcgaac cgaagaccgg cgtcaccgaa aaagatgccg atgtggtgag
     5521 cgcgatggtc gcgatggagg tggggcgcat atcgggcaga caggtcatcg gcgagaccga
     5581 gatgaggtcg ctcatggtgg gggaggaaaa gaaactctcg tgcggcgccg acgataccgc
     5641 ctgttatgcc gagatcggcg cggcgctcgg cgcccccgaa tcgatcatcg ggacgctcgc
     5701 caagatgggc gattactgga tactgtacct gcaacggctc aatgttcgca ccgcctcggt
     5761 gctggggcgc gccgagttca aggcgaaggg cgacatcaat ctacttgtcg agggtatccc
     5821 gccggcg
//
LOCUS       DAOQUE010000129         5807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15224_length_5807_cov_4.238178, whole genome shotgun sequence.
ACCESSION   DAOQUE010000129 DAOQUE010000000
VERSION     DAOQUE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15224_length_5807_cov_4.238178"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PKH10_05295"
     CDS             <1..332
                     /locus_tag="PKH10_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27582.1"
                     /translation="PAKVAAQYFVALTLSQAAAGTIRIVVNGTEADTVKPGEKAKIAE
                     ITKFLRPGPNKVLVMAGPEKVAVSVQVLIGTGKDNAGSLELTPAIDKQTGLSDKGLSE
                     TFDITAR"
     gene            346..1179
                     /locus_tag="PKH10_05300"
     CDS             346..1179
                     /locus_tag="PKH10_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT27583.1"
                     /translation="MRSLHLPILPTLFALLFVACATTETQTVSDETKGDSYYQLGIAA
                     FVKGDTVKAKLQITKAIEQAPLVPYYHNHLGLVYLQEKDFDRAQTCFEAAHKLDESYS
                     DALNNLGLLYLMKNDIPKAKEYFLRTIADQLYPYPHFAETNLGRALRMEKNYVEAEQH
                     IQRAIRLKGTHCDAYKELAMLYDEQGMNEKAVENYKKTIQYCPGHVEALYRGAVKLLV
                     LKDTALGTAWLKTCLEMERENTASIEIPFLRECANLARQYGVTLGKDTGDPRQQIDGG
                     Y"
     gene            1180..1686
                     /locus_tag="PKH10_05305"
     CDS             1180..1686
                     /locus_tag="PKH10_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27584.1"
                     /translation="METRTGLITFHRRTPKGGRYLLFAPEEGNIGLFARAEPFLLFDR
                     VEAVYVRRCDTLFLQEWTILDRSPLARHPERLVAAAYFAHLARCFTEGRGPEQAFVER
                     IPRLLEERIGPDTIVEAEVLWARAMGLRIDGDDRVGAVIHDHIGAAAYRLREQVTTVL
                     SRREESHA"
     gene            1679..2848
                     /locus_tag="PKH10_05310"
     CDS             1679..2848
                     /locus_tag="PKH10_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27585.1"
                     /translation="MHSGPMKGTAFITLVDQILRQRSRGRIVFTLRDNTKTLFFDNHA
                     VTGASSSDPSDYLGQYLVNEGAINLEQFNRAYQTQLETDVQMAQVLQLIGLAPVDKLR
                     ETIIVKIVDTAFIVSCWQEGTWEVDETYPTAVSGVEVRINIAELSSQLVRRQTDFAQI
                     LEMFNDLGDRPEVAMDGIDRERLKKLDQQVISFLAIGKTLRETLKAVPVHFYVLARHL
                     LSLYRDRMLRHGDGAPIAEVEIFDRLVRASGVARPQDTDLVASEEIAGVFRKAQEAMG
                     NKDYWKAASFYRMLCTVNPNNLVFKESLNNAEYHYVLHFYKEKFRPTAQIKKGTAPAG
                     GRCDPIEKKILDLVGEHTYVVRDLVGFFSDKFTEIKIINAIENLVRRGALVEAKV"
     gene            2845..3681
                     /locus_tag="PKH10_05315"
     CDS             2845..3681
                     /locus_tag="PKH10_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNT27586.1"
                     /translation="MKAAVIQSDCFLGDISRNLATHLRFAKMTAKDGATLVVFPELSL
                     SGYLLEDRVKDIALTAKQVTELFDGTGLPDIVIGVGFPERTRGAIYNAYALIRCGAKK
                     ADIIGIQRKVNLPTYGMFDEARHFRAGSSLRVHELPGGATAAVLICEDGWHPALPVAL
                     AQLPEGMPHLLVVPAASPSRGYRGERPSNLEGWDHAAEQFSRVYGAATLTAQRVGVED
                     SLIFAGGSTATDHNGTITRAALFTEETIIVDIDIEALSRLRAKAPVVSVRDREILRST
                     LA"
     gene            3674..4531
                     /locus_tag="PKH10_05320"
     CDS             3674..4531
                     /locus_tag="PKH10_05320"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNT27587.1"
                     /translation="MRRKTAGPLTLPPINGPLTADALVFFIRREVERAGFEGVVLGLS
                     GGIDSALVAFLAARAIGPRRVTGLMLPHRISSKGSVADAKMVVRTLGIRSHLLDISAA
                     VDGVERDLMTAPGIHRQGRLGNAMARVRMLALFDHSLAHRAMVLGTSNKSEIALGYAT
                     LFGDSASAVNPIGDLYKTDVYVLSRHLGVPRALLDKPPSADLWQGQTDEGEIGFRYAD
                     IDPVLHRLLDRRETPAALVARGADPVLVEFLSKKLLTTHYKRKIPVIAKLETRTFGVD
                     FLYAKDILS"
     gene            4509..5084
                     /locus_tag="PKH10_05325"
     CDS             4509..5084
                     /locus_tag="PKH10_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HNT27588.1"
                     /translation="MPKTSSRKKIALLGGKFDPPHLGHQLTIFLCLAKFGMDKVWLLP
                     SAAHPFGHTMSPLALRLKMCRLLALPWRGTGKVRILDDERRVDRRPVYTLDLVRHLKA
                     RYGDHDHHLVIGEDNWNARAEWKEFDELSKIVTPLVIGRGEGSAFPIALPDLSSTAVR
                     RAIADGAAWEHLVPAGVAEFVMRRGLYRPVA"
     gene            5168..>5807
                     /locus_tag="PKH10_05330"
     CDS             5168..>5807
                     /locus_tag="PKH10_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNT27589.1"
                     /translation="MRVWQWAVENFMNGELFFYVIPAVLLIVLILVIRLPDAEDRRDP
                     DADRDGKKDDDRSPVRPERAARHDTISLLPPERGREEPEVRVYYREPGSAEGSAVTGP
                     IPAEAPAAEVPPEAPRDWRDGLARTRGGFFSRLRSLFEASVVTDELVEKMEEILYSAD
                     LGVATVTSLMDEVRKARGALTTGDSVRDLLKDQIATILRTVEKPLVVPAQRPM"
BASE COUNT         1133 a         1845 c         1784 g         1045 t
ORIGIN      
        1 ccccggcgaa ggtcgcggcg caatatttcg tcgcgctgac gctctctcaa gcggcggcgg
       61 ggacgatacg gatcgtcgtc aacggcaccg aggccgatac cgtgaagccg ggagagaagg
      121 ccaagatagc cgagataacc aagttcctgc gcccgggacc caacaaggtg ctcgtgatgg
      181 ccggccccga aaaggtcgcc gtgtcggtgc aggtgctcat cggtacgggc aaggacaacg
      241 cgggcagtct ggaactcacg cccgcgatag acaagcagac ggggctttcc gacaaggggc
      301 tttccgagac cttcgacatc accgcacgat aggaggcgac accgcatgag atcccttcat
      361 ctcccgatcc ttccgacgct cttcgcgctc ctgttcgtcg cctgcgccac caccgagacc
      421 cagaccgtca gcgacgaaac gaagggcgac agttactacc aactcggcat cgcggcgttc
      481 gtcaagggcg atacggtcaa ggcgaaactc cagataacca aggcgatcga gcaggcgccg
      541 ctcgtcccgt actatcacaa ccacctcggg ctcgtctatc tgcaggaaaa ggatttcgac
      601 cgcgctcaga cctgtttcga ggcggctcac aaactcgacg agagttactc cgacgcgctc
      661 aacaacctcg gactcctcta cctcatgaag aatgacatac cgaaggcgaa ggaatatttc
      721 ctgcggacga tcgccgacca gttgtatccc tacccgcatt tcgccgaaac gaatctcggt
      781 cgcgcgctcc ggatggaaaa gaactatgtc gaggcggaac agcatatcca gagggcgata
      841 cggctcaaag ggacgcactg cgacgcctac aaggaactcg ccatgctcta tgacgagcag
      901 gggatgaacg agaaggcggt ggagaactac aagaagacca tccagtactg tccgggccat
      961 gtcgaggcgc tttatcgcgg ggcggtcaaa ctgttggtgc tcaaggatac cgcgctcggc
     1021 accgcgtggc tcaagacctg tctggaaatg gagcgcgaga acaccgcgtc gatcgagata
     1081 ccgttcctgc gtgaatgcgc caatcttgcc cgtcagtatg gcgtgaccct ggggaaggat
     1141 accggtgacc cgcgccagca gatagacggg gggtactgag tggaaacgcg caccggactc
     1201 atcacctttc accgccgtac ccccaagggc ggcagatacc ttttgttcgc gccggaggag
     1261 gggaatatcg gcctgttcgc ccgcgccgag ccgttcctgc tgttcgaccg cgtcgaggcg
     1321 gtctatgtcc gccggtgcga tacgctgttc ctgcaggagt ggaccatcct cgatcgctcg
     1381 ccgctcgccc gtcacccgga acggctggtc gctgccgctt acttcgccca cctcgcacgc
     1441 tgtttcactg aaggtcgcgg gccggaacag gcgttcgtcg agcggatacc tcgcctgctc
     1501 gaggaacgga taggtcctga taccatcgtc gaggccgagg tgctctgggc ccgggcgatg
     1561 ggactgcgga tcgacggcga cgaccgcgtg ggagccgtca tacatgatca catcggtgcc
     1621 gcggcgtatc gcctgcgcga acaggtgacg acggtccttt caagaaggga ggaaagccat
     1681 gcatagcggg cccatgaagg ggacggcgtt catcacgctc gtcgaccaga tactgagaca
     1741 gcggtcgcgc ggtcgcatcg tcttcaccct gcgagacaac acgaaaacac tctttttcga
     1801 caaccatgcg gtgaccgggg cgagttccag cgacccgagc gactacctcg gccagtacct
     1861 cgtcaatgag ggggccatca acctcgaaca gttcaaccgc gcctaccaga cccagttgga
     1921 gaccgatgtg cagatggcac aggtcctcca actcatcggg ctggcgccgg tcgacaaact
     1981 gcgcgagacc atcatcgtca agatcgtcga caccgcgttc atcgtgtcgt gctggcagga
     2041 gggtacttgg gaggtcgacg agacttatcc cacggcggtg agcggcgtcg aggtgcgtat
     2101 caatatcgcc gaactgtcgt cccaactggt gcggcggcag accgatttcg ctcagatact
     2161 cgagatgttc aacgatctcg gcgatcggcc ggaggtcgcc atggatggta tcgatcgcga
     2221 acgactcaag aaactcgacc agcaggtgat aagttttctc gccatcggca agacgctccg
     2281 cgagacgctg aaagcggtac cggtccattt ttatgtgctc gcccgacacc tcctttccct
     2341 gtaccgcgac cgcatgctcc ggcacggtga cggcgcgccg atcgccgagg tcgagatatt
     2401 cgatcggctc gtgagggcga gtggcgtggc gcggccgcag gacaccgatc tcgtggcgag
     2461 cgaggagatc gccggcgtgt tccgcaaggc gcaggaggcg atggggaata aggactactg
     2521 gaaggcggcc tccttctacc gtatgctctg taccgtgaat ccgaacaatc tcgtgttcaa
     2581 ggagtcgctc aacaacgccg aataccacta tgtgctccat ttctacaagg agaagttccg
     2641 gccgaccgcc cagataaaaa aaggtaccgc tccggcggga gggagatgcg acccgataga
     2701 gaagaagata ctcgatctgg tcggcgaaca tacctatgtg gtgcgtgatc tcgtcgggtt
     2761 cttctctgac aaattcaccg agataaagat aatcaacgcg atcgagaatc tcgtccgccg
     2821 tggcgctctg gtcgaggcga aagtatgaag gccgccgtca tccaatccga ttgttttctc
     2881 ggcgacatct cgcgcaatct cgccacccat ctgcggttcg cgaagatgac tgcgaaagat
     2941 ggcgcgacgc ttgtcgtgtt ccccgaattg tcgctgtcgg ggtatctcct cgaggaccgc
     3001 gtgaaggata tcgccctgac ggcgaaacag gtgaccgagc tcttcgacgg taccggcctg
     3061 cccgacatcg tcatcggtgt cggctttccc gaaagaacgc gcggcgccat ctataatgcc
     3121 tacgccctca tccggtgcgg cgcgaaaaag gcggatatca tcggcatcca gcgcaaggtg
     3181 aacctgccga cctacggcat gttcgacgaa gcgcgtcatt tccgcgccgg ttcttcgctc
     3241 cgcgtccacg aactgccggg tggcgcgacc gccgcggtgc tcatctgcga ggacggctgg
     3301 cacccggccc tgccggtggc gctcgcgcaa cttcccgaag ggatgccgca tctgctcgtc
     3361 gttcccgccg caagcccgtc gcgcggctac cgtggcgaac ggccgtcgaa cctcgaaggg
     3421 tgggaccacg ccgctgaaca gttttcccgc gtctacggtg ccgcgacgct caccgcccag
     3481 cgggtcggcg tcgaggacag tcttatcttc gcgggcggct cgacggcgac cgaccataac
     3541 gggaccatca cgcgggcggc actgttcacc gaagagacga tcatcgtcga catcgatata
     3601 gaggcgctct cgcgactccg cgccaaggcg cccgtcgtct cggtgcgcga ccgcgagatc
     3661 ctcaggagca cccttgcgta ggaagaccgc cggcccgctt accctgccgc cgatcaacgg
     3721 accattgacc gccgatgcgc tcgtcttctt catccggcgc gaggttgagc gcgcaggttt
     3781 cgaaggcgtc gtgctcggtc tttcgggcgg tatcgactcg gcgctcgttg cgtttctcgc
     3841 ggcccgcgcc ataggtcccc ggcgggtaac ggggctcatg ttgccccacc gcatctcgtc
     3901 aaaaggctcg gtcgccgatg caaaaatggt cgtccgtacc ctcggcatcc ggtcacacct
     3961 gctcgacata tcggcggcgg tcgacggcgt cgagcgcgac ctcatgaccg cgcccggcat
     4021 ccaccgacag ggacggctcg gcaacgcgat ggcgcgggtg cggatgctgg ctcttttcga
     4081 ccactctctg gcccatcgcg cgatggtcct cggcaccagc aacaaaagcg agatagcgct
     4141 cggctacgcg acgcttttcg gcgacagcgc ctcggcggtc aacccgatcg gtgacctcta
     4201 caagaccgat gtgtacgtcc tctcgcgtca tctcggcgtc ccgcgggcgc ttcttgacaa
     4261 gccgccgtcg gccgacctgt ggcaggggca gaccgacgaa ggggagatag ggttccgcta
     4321 cgctgacatc gatcctgtgc ttcaccgtct cctcgaccgc cgtgaaacgc ccgccgccct
     4381 tgtcgcgcgc ggcgccgacc cggtgcttgt agagttcctt tcaaaaaaac tgctcacgac
     4441 gcattacaaa cgcaagatac cggtcatcgc gaaactcgag acgcggacct tcggggtcga
     4501 ctttctctat gccaaagaca tcctctcgta agaagatcgc cctgctgggc ggcaaattcg
     4561 atccgcccca tctggggcat caactgacga tattcctgtg cctcgccaaa ttcggtatgg
     4621 acaaggtctg gctgctgccg agcgccgccc accccttcgg tcatacgatg agcccgttgg
     4681 ccctgcgtct caagatgtgt cgcctgctcg ccctgccgtg gcgcggcacc ggaaaggtgc
     4741 gcatcctcga tgacgagcgc cgggtcgacc ggcggccggt ctacaccctt gatctggtgc
     4801 gccacctcaa ggcgcgttat ggcgaccacg atcatcacct cgtcatcggg gaggataact
     4861 ggaacgcccg cgccgagtgg aaggagttcg acgaactttc gaaaatcgtc acgccgctcg
     4921 tcatcggccg cggcgagggt tcggcgttcc cgatagcgct tccggaccta tcgagcaccg
     4981 cggttcgacg ggccatagcg gacggcgcgg cgtgggaaca cctcgtcccc gccggggtgg
     5041 ccgagttcgt gatgaggcgg ggcctgtacc gtcctgtagc ataaggcgac gacctgtttt
     5101 ttattgacag gagcgcgccg tcgctttatc cttctccggt ggaaagtatt tcgaggggat
     5161 gttcgatatg cgcgtctggc agtgggcggt agagaatttc atgaacggcg aattgttctt
     5221 ctatgtgatc cccgccgtgc tccttatcgt gctcatcctt gtgatacggc ttcccgacgc
     5281 cgaggaccgc cgcgatcccg acgccgaccg cgacgggaaa aaggatgacg acagatctcc
     5341 ggtccggccc gaacgcgccg cccggcacga cacgatatcg cttctgccgc ccgaacgcgg
     5401 cagggaggag ccggaggtcc gggtttatta tcgtgagccc ggctccgctg aaggctccgc
     5461 cgtgacaggc ccgatacccg cggaagcccc cgccgcggag gtgccgccgg aggcgccgcg
     5521 tgactggcgc gacggtcttg cccggacgcg cggcggattc ttctctcggt tacggtccct
     5581 tttcgaagca agcgtcgtca ccgacgaact cgtcgagaag atggaagaga tactctacag
     5641 cgccgatctc ggcgtcgcga cggtgacctc cctgatggac gaggtgcgca aggcgcgcgg
     5701 cgcgctgacg accggcgatt cggtgcgcga cctgctcaag gaccagatag caacgatact
     5761 gagaacggtc gaaaaaccgc ttgtggtgcc cgcacagcgg cccatgg
//
LOCUS       DAOQUE010000130         5797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15258_length_5797_cov_4.786834, whole genome shotgun sequence.
ACCESSION   DAOQUE010000130 DAOQUE010000000
VERSION     DAOQUE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15258_length_5797_cov_4.786834"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKH10_05335"
     CDS             <1..716
                     /locus_tag="PKH10_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT27590.1"
                     /translation="FQLFDSEENMVRVDMSEYMEKHSVSRLIGAPPGYVGYDEGGQLT
                     EAVRRKPYSIVLLDEIEKAHPDVFNILLQILEDGRLTDNQGRTVSFRNTIVIMTSNLG
                     SDLLSGKGDTVSEKEREQVMARVRASFKPEFLNRIDDIVIFNPLGKKHLREIVGIMLS
                     DIAGRLAEREITLDFTDAAKDLVIEESFEPEYGARPIRRYLERVIETELATRIIKGEL
                     GDGDRITVDRKGDEFVWKR"
     gene            969..1544
                     /locus_tag="PKH10_05340"
     CDS             969..1544
                     /locus_tag="PKH10_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27591.1"
                     /translation="MKRATLLVLVGVLFLVPLQLSAADEKTVTVEIEKGVLFKITTES
                     FVPEKHKLTYSDTPANDEGRRCVIAIDGFPFFGDVGCCPPEIKVKSIALTIHGKNYTL
                     DSSFMYNPLFRSHRYVHFVRKGENIFMIRANFSDGGGSYCAEWYIGNGKSARTILTDD
                     EGLCGGFLPDEKEQKKEKKDGVIPPPRHSEE"
     gene            1587..1958
                     /locus_tag="PKH10_05345"
     CDS             1587..1958
                     /locus_tag="PKH10_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HNT27592.1"
                     /translation="MGKEFFSKPSTKEKRRALRREMTDAERALWSKLSNRQLGGLKFR
                     RQYGLGEYIADFYCPESRLVVELDGGQHYQEEGKEYDALRAQYMSAMGIRTIRFPNNE
                     VLANLAGVLAEIERSAKGPTA"
     gene            2189..2884
                     /locus_tag="PKH10_05350"
     CDS             2189..2884
                     /locus_tag="PKH10_05350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT27593.1"
                     /translation="MTMLRHIAILFLVFGLFACGEDGTGTTTDAAADLDAMAADAADD
                     ALAADDLLPDEDTAPLVTRRAVLETTLGDITIGLYGDAMPVTSGNFEAYIEEDFFTGL
                     IIHRVVPGFVIQGGGYDAELVAKETHDPIDFERNPAVRHVKYAISMARTANIDSATSQ
                     FFITLDAQPRLDYDSDDDFENKDKYPCAAFGIVTDGFEVVDAIGAVETSTQNGLTDVP
                     ITPVVITGTRFAD"
     gene            2917..3393
                     /locus_tag="PKH10_05355"
     CDS             2917..3393
                     /locus_tag="PKH10_05355"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016114652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNT27594.1"
                     /translation="MTIYDLPVKTIAGRETTLAEYRGQVLLIVNVASKCGFTPQYKGL
                     QALYEKYRERGFTVLGFPCNQFAGQEPGTSEEIAQFCEINFGVTFPLFEKITVNGKKA
                     HPLYAYLKEQAPGLLGSTAIKWNFTKFLVDRQGNVLKRFAPAEPPESLEQEIEKVL"
     gene            3427..4017
                     /locus_tag="PKH10_05360"
     CDS             3427..4017
                     /locus_tag="PKH10_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HNT27595.1"
                     /translation="MLDVLKRDVFFVKEHLGIFKAANNYDILDPETGAVVMECREENL
                     GFFTKLLRFTDYKTYTPFDVQVRTPDGQQVVRVTRGISIFLSKVQVFDEKDAYIGGFN
                     QRLFSIGGAFTVVDASGNPVCDLKGSWTGWDFRFLSGGVELAHVSKKWAGVGKELFTS
                     ADNYVLEISKSVAPDSAARQLILAAVMCIDMVLKER"
     gene            complement(4037..4642)
                     /locus_tag="PKH10_05365"
     CDS             complement(4037..4642)
                     /locus_tag="PKH10_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27596.1"
                     /translation="MHSQMLKVARSIIWLLIPLVVLGCKKEEEKKHALTIIPALEQLE
                     EDFCLEKNLYLQNVDHYRDMDKFLTKECPCRNDPAEDAKECDLCMKIASAYFPYHEAM
                     PLINCGSWCEKEWKEKVSQTNSPHLVQDQKGFIVLSFWSEDEYTCYYNIDRKCLRIDD
                     SPHDMATYKFALDAKTLKPIETAQTKELRELCIEKKKQKNP"
     gene            complement(4733..>5797)
                     /locus_tag="PKH10_05370"
     CDS             complement(4733..>5797)
                     /locus_tag="PKH10_05370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNT27597.1"
                     /translation="LREEWQVFDPLVKADLDILEKVREGDLFVVNRDRADTKWIVGFE
                     LDNGPYSFYLYDRASKKAEFLFTHQPELEKYKLATMKPVVIRSRDGLDLVSYLTLPVG
                     IEPKGLPLILYVHGGPWHRDGWGYDPGAQWFANRGYAVLQVNFRGSTGFGKNFVNAGN
                     GEWGVGKMQHDLTDAVQWAIKEGIADPKKVAIFGGSYGGYATLAGLTFTPDLYACGVD
                     IVGPSNLQTLMATIPPYWKPFKMQMLKRIGDVENDQEFNKKISPLFHVDVITVPLMIG
                     QGQNDPRVKVAESDQIVAAMRAKKIPVTYILYPDEGHGFARPENRMDFNGRAEQFLAQ
                     CLGGRAEPYQKKEGTTAQEK"
BASE COUNT         1253 a         1584 c         1713 g         1247 t
ORIGIN      
        1 cgttccagct gttcgactcc gaggagaaca tggtccgcgt cgacatgtcg gaatacatgg
       61 agaagcattc ggtgtcgcgg ctcatcggcg cgcctcccgg ctatgtcggc tacgacgaag
      121 ggggacaact gaccgaggcg gtgcgacgca aaccctacag catcgtgctg ctcgacgaga
      181 tagagaaggc gcatcccgat gtgtttaaca tcctcctgca gatactggag gacgggcggc
      241 tcaccgacaa tcaggggcgc accgtcagtt tccgcaacac tatcgtcatc atgacctcga
      301 atctcggaag cgatctgctc tcgggaaagg gcgacaccgt ttccgaaaag gaacgcgaac
      361 aggtcatggc gcgcgtgcgg gcatcgttca agccggagtt cctcaaccgc atcgacgata
      421 tcgtcatctt caacccgctc gggaaaaagc acctgcgcga gatcgtcggc atcatgctct
      481 ccgacatcgc gggacggctc gccgaacgcg agataacact cgacttcacc gacgccgcca
      541 aagatctggt catcgaggag agtttcgagc ccgaatacgg cgcgcggccg atccggcgct
      601 atctggaacg ggtcatcgag accgaactgg cgacgcgcat catcaaaggt gaactgggcg
      661 acggcgaccg tatcaccgtc gaccggaaag gcgacgagtt cgtgtggaaa cggtgacaac
      721 tcgcgcacaa caccctcacc cccgccctct ccctagacag ggcgaggggg tatatggaaa
      781 acgtcatcac ataaaaccct cacctcattc ctctccctcg atagggagag gaagtccgat
      841 cactccttct ccctgtggag ggagaaggtg ggatgagggt gttccggagg aaagtaccac
      901 gccatttctc ttgcttctca gccgcgcggc gatataattg tgacggtcat taagggagtg
      961 gggatgtcat gaaaagagca acactcttag ttctagtcgg cgtacttttc ttggttcctc
     1021 tccagttgag cgctgctgac gagaaaacgg tcacggtcga gattgaaaaa ggcgtgctct
     1081 tcaagatcac tacggaatcc tttgtccctg aaaagcataa actcacctat tctgacacac
     1141 ctgccaatga cgaaggcaga agatgcgtca ttgcgattga tggattcccg tttttcggcg
     1201 atgtggggtg ttgcccgccc gaaataaaag ttaaatctat cgcgctgacg attcatggca
     1261 agaactacac cttggattca tcgttcatgt ataatccatt gttccgcagt catcgctatg
     1321 tgcatttcgt ccgcaaggga gaaaacatat ttatgatccg tgcaaacttc tctgacggtg
     1381 gtgggtctta ctgcgcagag tggtatatcg gcaacgggaa gtccgccaga acaatcctga
     1441 cggacgacga gggcttatgc gggggatttc ttcccgacga gaaagagcag aaaaaagaaa
     1501 agaaggacgg ggtaatcccg cctccgcgtc attcggaaga ataacacgcg acgatatgat
     1561 cgggatgttt cgagggacat agtagagtgg gcaaggaatt cttctccaag ccaagcacca
     1621 aagaaaagcg ccgagccctt cgcagggaaa tgaccgacgc cgaacgggcg ttgtggagca
     1681 agttgagcaa ccgtcaactc ggcggcttga aattccgccg ccaatacgga ctcggcgaat
     1741 acatcgctga tttctattgc cccgaatcgc gattggtcgt ggagctggac ggcggccagc
     1801 attatcagga agagggaaag gagtacgacg cacttcgcgc acagtacatg tccgccatgg
     1861 gtatccgcac catacggttc ccgaacaatg aggtgcttgc gaatctcgcc ggggtgttgg
     1921 ccgagatcga gcggtcggcg aaggggccga cggcctaaca aaaaccctca cctcaatcct
     1981 ctccctcgac agggagagga agcccgatca ctccttctcc ctgtggaggg agaagggggg
     2041 atgagggtgt tgcggagaga ctccttcgct cttctccctg tggagggaga aggtcgggat
     2101 gagggtgttc cggggaaaga tgtctttccg tttctcttgc ttctccgccg cgcggcgata
     2161 tactcctttg ttcatcaggg aggcacccat gaccatgctc aggcatatcg cgatcctttt
     2221 tcttgttttc ggcctcttcg cctgtgggga agacggtacc ggaacgacga ccgatgccgc
     2281 cgccgacctc gacgccatgg ccgccgacgc cgcggacgac gcgcttgcgg cggacgatct
     2341 cctgcccgac gaggacacgg cgcctctcgt gacgcgccgc gccgttctcg aaacgacgct
     2401 cggcgacatc accatcggcc tctacggtga cgcgatgccg gtgaccagcg gcaatttcga
     2461 ggcctatatc gaagaggatt tcttcacggg actcattatc caccgcgtcg ttcccggctt
     2521 tgtgatacag gggggcggct atgacgccga actggtcgcg aaagagacgc acgacccgat
     2581 agatttcgag cgcaacccgg cggtcagaca tgtgaagtac gccatttcga tggcgcggac
     2641 cgcgaatatc gattcggcca cctcgcagtt cttcatcacg ctcgatgcac agccgcgtct
     2701 tgattacgat tcggatgacg atttcgagaa caaggacaag tacccgtgcg ccgccttcgg
     2761 tatcgtcacc gacggcttcg aagtggtcga cgcgataggg gcggtcgaga cctcgacgca
     2821 gaacggcctc accgatgtgc cgatcacgcc cgtggtcatc accggcaccc gtttcgccga
     2881 ctagagggat gtcagataac ggagggagat accgccatga ccatctacga tcttccggtg
     2941 aagacgatcg cggggcggga gacgacgctg gcggagtacc gtgggcaggt cctgctcatc
     3001 gtcaatgtcg cgagcaagtg cggtttcacg ccgcagtaca aagggttgca ggcgctctac
     3061 gagaagtacc gggagcgcgg ttttaccgta ctggggttcc cgtgcaatca gttcgcggga
     3121 caggagccgg ggaccagcga agagatagcg cagttctgcg agatcaattt cggggtgacc
     3181 tttccgctct tcgagaagat cacggtgaac gggaaaaagg cccacccgct ctacgcttac
     3241 ctcaaggaac aggcgccggg gcttctcggc tcgaccgcca tcaagtggaa tttcaccaaa
     3301 ttcctcgtcg accggcaggg gaatgtgctt aaacgcttcg ccccggccga accgccggaa
     3361 tcgctggaac aggaaataga gaaggtgctg tagggccgca gatattatct ttttggggag
     3421 gggaccatgc tcgatgtatt gaagcgcgat gtgttcttcg tcaaggagca tctcgggata
     3481 ttcaaggcgg cgaacaacta cgacattctg gacccggaga ccggggcggt ggtgatggaa
     3541 tgccgcgagg agaatctggg attcttcacc aagctgctgc gctttaccga ctacaagacc
     3601 tacacgccgt tcgacgtgca ggtcaggacg ccggatggac agcaggtggt ccgcgtgacg
     3661 cggggcatct ccatctttct gtcgaaggtg caggtgttcg acgagaagga cgcctacatc
     3721 ggcggcttca accagcggct tttctccatc ggcggcgcct tcacggtggt cgatgcgtcg
     3781 ggaaatccgg tgtgcgatct gaagggttcg tggacggggt gggatttccg gttcctttcg
     3841 gggggcgtgg agttggcgca tgtgtcgaag aagtgggcgg gggtcggcaa ggagctgttc
     3901 accagcgccg acaactatgt gctggagata tcgaagtcgg tcgcgcccga cagtgcggcg
     3961 cggcaactca tcctcgccgc cgtcatgtgc atcgacatgg tgctcaagga acggtgatag
     4021 gttgatttcg tagaaactac ggattcttct gttttttctt ttctatgcac aattcgcgca
     4081 attctttggt ttgggcagtt tcgattggtt tgagcgtttt cgcatcaagg gcaaatttgt
     4141 aagttgccat atcatgagga ctatcatcta ttctgaggca cttcctatct atattgtaat
     4201 agcatgtgta ctcgtcttcg ctccagaaag aaaggactat gaaccctttt tgatcctgca
     4261 caaggtgggg agagttggtt tgagacacct tctctttcca ttctttttca caccaactcc
     4321 cacaatttat gagaggcatg gcctcatgat agggaaaata tgcagaagcg attttcatgc
     4381 agaggtcaca ctctttggca tcttcagccg gatcgtttcg gcaagggcat tcttttgtta
     4441 aaaacttgtc catatcgcgg taatgatcga cattctgcaa gtaaaggttt ttctcgaggc
     4501 agaagtcctc ttctaactgt tcaagcgctg gaatgatagt aagggcgtgc tttttctctt
     4561 cctctttttt gcagcccaac acgacaagag gaataagtag ccagattatg cttcgcgcca
     4621 ctttgagcat ctgtgagtgc atgatatccc cccgaatatt cttcctaaac cagcgacagt
     4681 atgccgataa gttattcttt ttgcaagaat acggggggac gaagcgaaag atctacttct
     4741 cctgcgcggt ggtgccttct ttcttctggt acggttcggc acggccgcct aagcactgcg
     4801 caaggaactg ttcggcgcgc ccgttgaaat ccatccggtt ctcgggccgc gcgaacccat
     4861 gcccctcatc gggatagaga atgtaggtga ccgggatctt cttcgcccgc atcgccgcga
     4921 ctatctggtc cgactcggcg actttcacgc gcggatcgtt ctgaccctgc ccgatcatga
     4981 gtggcaccgt gatgacgtcg acatggaaga ggggcgatat cttcttgttg aactcctgat
     5041 cgttctccac atcgccgatg cgcttcagca tctgcatctt gaacggcttc cagtaaggag
     5101 gaatggtcgc catgagcgtc tggaggttcg acggcccgac gatatcgacg ccgcaggcat
     5161 agagatcggg ggtgaaggtg aggccggcga gcgtcgcgta gccgccgtag gagccgccga
     5221 atatcgccac cttcttcggg tcggcgatgc cctcttttat cgcccactgc accgcgtcgg
     5281 tgaggtcgtg ctgcatcttg cccacgcccc attcaccgtt cccggcgttc acgaagttct
     5341 tgccgaagcc ggtcgagccg cggaaattga cctgcaacac cgcgtagccg cggttggcga
     5401 accactgcgc gccggggtcg tagccccagc cgtcgcgatg ccacgggccg ccgtggacat
     5461 agaggatgag cggcagtccc ttcggttcga tgccgaccgg cagggtgagg taggaaacga
     5521 ggtcgagccc gtcgcgcgac ctgatgacga ccggcttcat ggtggcgagt ttatatttct
     5581 ccagttcggg ctggtgggtg aagagaaatt ctgccttctt ggaggcgcgg tcgtagaggt
     5641 agaacgagta gggaccattg tcgagttcga agccgacgat ccatttggtg tcggcgcggt
     5701 cgcggttgac gacgaagagg tccccttcgc gcaccttctc aaggatgtcg aggtcggcct
     5761 tgaccagcgg gtcgaacacc tgccactcct cgcgcag
//
LOCUS       DAOQUE010000131         5788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15293_length_5788_cov_4.126286, whole genome shotgun sequence.
ACCESSION   DAOQUE010000131 DAOQUE010000000
VERSION     DAOQUE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15293_length_5788_cov_4.126286"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..703)
                     /locus_tag="PKH10_05375"
     CDS             complement(23..703)
                     /locus_tag="PKH10_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27598.1"
                     /translation="MEQRMPLLLSFFLLVFAAVLLAPYFSDRSFRGRVTVRWDASSDS
                     IPPDVTVLMVPGRGYESLFADFLGETAWQTIADLFRKKAGIAVFPTGDILVVKKKGDA
                     MLSVALHHYLSRVSGVSYTFRQRGREWRLTKAPIILEEQTMIRTFVMHDEFSRDFPEF
                     YELIRPAMQWDWGLLHQLRPGDSISFMLRGVFDHKILIDLYDLTGLSVKSERYGQFSL
                     FKPPGCRA"
     gene            842..991
                     /gene="rpmH"
                     /locus_tag="PKH10_05380"
     CDS             842..991
                     /gene="rpmH"
                     /locus_tag="PKH10_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNT27599.1"
                     /translation="MMPTYRPSRVHRKKTHGFRRKMRTADGRKVLQSRRRKGRKRIAV
                     DIYKK"
     gene            1008..1373
                     /gene="rnpA"
                     /locus_tag="PKH10_05385"
     CDS             1008..1373
                     /gene="rnpA"
                     /locus_tag="PKH10_05385"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNT27600.1"
                     /translation="MGTFSFSRAERIRSRKEFQGLKSKGAVFKTRKLVFNFAATTGPS
                     RLGVVATKKMGNAVFRNRIKRWLREAFRLNKQAMARPMDLVIVPRVSELSFADIEQDL
                     RFFQQWHREKNEKNAHRAH"
     gene            1345..1557
                     /gene="yidD"
                     /locus_tag="PKH10_05390"
     CDS             1345..1557
                     /gene="yidD"
                     /locus_tag="PKH10_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNT27601.1"
                     /translation="MKRTLIALIEGYRRYLSPHLGRNCRFYPTCSAYTIEAIEKKGVA
                     KGLLLGAWRILRCNPWNPRFGHDPVK"
     gene            1567..3219
                     /gene="yidC"
                     /locus_tag="PKH10_05395"
     CDS             1567..3219
                     /gene="yidC"
                     /locus_tag="PKH10_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNT27602.1"
                     /translation="MNKNMILAVVLSVVVLYVWGEFFAPKPQPLPEQSGSVENSAVTS
                     PDNQSAKPQEKNDSALQQGPDAATQPGAASGSAVARPADGDLFRHGTLAVTAQNPAQN
                     GALNGVAMTMEYTSKGGRLTALPVTGEKYRNKKLDMLAALRDDAYFPEMSSVFAEEPS
                     YAVEEASGDAITFRYEQEGLIEWKTLRFSGDYLLTVSKKVMNNSVAPINWRPALRYRS
                     IHENKEVFSSYGKRFEFLVKTTSEGMKEPSSGEKLKEETQGRQIQWAGINYGFFLYAA
                     VGKEQEALTVDGVIDSKQNLTVLTAAHDEFTIAPGQTAEAVFTVFLGPKEVRLLEQAG
                     NDLRASASLGWFGFLAEPLLWILNFFYDFVKNYGIAIILLTILVKLLLWPLSDASYKS
                     MAKMKKLMPKVEELKKKYKEDKETLNKEIMLLYQKEGVNPLGGCLPILIQMPIYIALY
                     SMLNNAVELYNAGFLPFWLVDLSEKDPYYILPVALGGFMFLQQKMTPTQMDNQQAKIM
                     LYVMPVMFAGFMLFLPAGLNLYILANTLLGILQQWLVTKRYG"
     gene            3300..4673
                     /locus_tag="PKH10_05400"
     CDS             3300..4673
                     /locus_tag="PKH10_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNT27603.1"
                     /translation="MRLSLGLLSLLALFTIAACENDVHSLAPWSADSYYGDPEGYDWD
                     TDAAGMWDRRPNLAACSNEDVNLWILDKLWDMYLWYDHLADDIDFRVELEPGDFLGAV
                     KYKESDHFSYLSKKEDHDDYYGGRYYGFGFSFRKFPDYVVYVTLVIPGSNAETAGMRR
                     GQRIIAIDGKTVQEIEDNNLWSTVTSRPEDKKDIPLEYVVEEEGEEKTLTLYAGDVTA
                     PSIYKTDILDDDDRKVGYLMLKSFINSTNGELDTAFTAFKAAGVTDLVVDLRYNGGGW
                     LDGSEHLASLITGGELKDELFTRLTYNKKHQDYNSDYHFKDFAQSLGLQKVVFIATGG
                     TASASEVVINGLKPYDKVAIVGGKTYGKPVGMNPMSYCDYTLVPITFKLANADGYGDY
                     FTGMPADCEADDDVTHDFGDARETSLAAALSYLRDGTCPAAPAVYAWNPDEIDVRTVP
                     YRLGWLF"
     gene            complement(4693..>5788)
                     /locus_tag="PKH10_05405"
     CDS             complement(4693..>5788)
                     /locus_tag="PKH10_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor family
                     protein"
                     /protein_id="HNT27604.1"
                     /translation="EWPDGSDGEWPDGSDGEWPDGSDGEWPDGSDDEWPDWSDGEWPD
                     GSDGEWPDWSDGEWPDGSDSEWPDWSDGEWPDDDMVNSCGSNENCEETEYCAKPDGTC
                     MTFLAGTCEERPQMCPPLYAPVCGCDGNTYSSDCDAHAAGVNVDHAGECGTNPGCYSN
                     EECNDGSGMDPQFCLYETGVCTGPGTCTEIPAMCGDIYSPVCGCDMVTYDNECLANQA
                     GFSVMYEGVCKEEKFSTLSFYYDQSTMNAPEAKVVVVNGDATVEFPTADLMTRTPFTG
                     GVYFRTTFYGTTDDGSKIEFQLRAYSIGWSIPKTFSLDGTNNYARWTKTGNIVMGDLS
                     GDVTITQYARDNEIVTLMEVGGDMLTFTPN"
BASE COUNT         1230 a         1800 c         1684 g         1074 t
ORIGIN      
        1 ctggggatct cggcacccac cgtcaagcgc gacacccggg cggcttgaag agcgagaact
       61 gaccgtaccg ttcgcttttc accgaaagtc cggtaagatc gtagagatct atgagtatct
      121 tatgatcgaa caccccgcgc aacatgaacg atatcgagtc gccgggccgg agttgatgca
      181 acagccccca gtcccactgc atggccgggc gtatgagttc gtagaattcg gggaaatcac
      241 gcgagaattc gtcgtgcatg acgaaggtgc ggatcatcgt ctgttcctcg aggatgatgg
      301 gtgccttggt cagccgccat tcccgccccc gctgtcggaa agtataggag acccccgaca
      361 cgcgagacag ataatggtgc agagctaccg aaagcatcgc gtcgcccttt ttcttgacga
      421 cgaggatgtc tccggtcggg aacacggcga tacccgcctt tttgcggaac agatcggcta
      481 tcgtctgcca cgccgtttcg ccgaggaaat cggcgaatag cgactcgtac cccctccccg
      541 gcaccatgag caccgtcaca tcgggcggta tgctatcgga ggatgcatcc cagcggacgg
      601 tcacccgacc gcggaacgag cggtcggaga agtagggagc gagcagcacc gccgcgaaga
      661 cgagcagaaa gaatgagagc agtaacggca tcctctgttc cacgcggcgc tccaaaaacg
      721 aaagaccgca ggattgtaac ggccgtgcgg cggaaagtca aatgtactca tttttgttga
      781 caatcggacg gtcaccggta tattccttcc gtttttatgg ttcatatgaa ggagttgccc
      841 catgatgccg acatatcgtc cgagtcgggt gcaccgcaag aagacccacg ggttcagacg
      901 gaaaatgcgc accgccgacg gtcgcaaggt cctgcagtcg cgccgccgca aggggcgcaa
      961 acgcatcgcc gttgacatct acaagaagtg atcgatcgtc gcgctccgtg gggacctttt
     1021 ccttttcacg cgcagagcgg atacgatcgc gaaaagaatt tcagggactt aagtcaaaag
     1081 gggcggtttt caaaacgcgt aaactggttt tcaacttcgc cgccacgaca ggcccctcgc
     1141 gactcggcgt ggtcgccacg aaaaagatgg ggaatgcggt gttccgcaac cggatcaagc
     1201 ggtggctccg ggaggcgttc cgcctcaaca agcaggcgat ggcccggccg atggacctcg
     1261 tgatcgtgcc gcgcgtctcg gaactctcgt tcgccgatat cgagcaggac ctccgctttt
     1321 tccagcagtg gcacagggaa aaaaatgaaa agaacgctca tcgcgctcat tgagggatac
     1381 cgtcggtatc tgtcgcccca cctcggcagg aactgccggt tctatcccac ctgctcggcc
     1441 tataccatcg aggcgatcga aaagaagggg gtggcgaagg ggctcctgct cggcgcgtgg
     1501 cgcatactcc gctgtaaccc gtggaacccc cgtttcggac acgaccccgt gaagtgaggt
     1561 gatccgatga acaagaacat gatactggcg gtcgtcctct cggtcgtcgt cctctatgtc
     1621 tggggcgagt tcttcgcccc caaacctcag ccgctccccg aacagtccgg ctccgttgag
     1681 aactccgccg taacaagccc ggacaaccag agcgcgaaac cgcaggagaa gaacgactcc
     1741 gcgctccagc agggccccga cgccgccacg cagcccggcg ccgcctccgg ctccgccgtg
     1801 gcaaggccgg cggacggcga tctgttccgg cacggcaccc tcgccgtgac ggcgcagaac
     1861 cccgcgcaga acggggcgct gaacggcgtg gcgatgacga tggagtacac atcgaagggc
     1921 ggccgactca cggcgctccc ggtcaccggc gagaaatacc ggaacaagaa actcgacatg
     1981 ctcgcggcgc tccgcgacga cgcctatttc ccagagatgt cgtcggtctt cgccgaagaa
     2041 ccttcctacg ccgtcgaaga ggcgtcgggc gacgcgatca ccttccgcta cgaacaggag
     2101 ggactcatcg agtggaagac cctgcgattc tcgggtgact acctgttgac cgtttcgaaa
     2161 aaggtgatga ataactcggt cgccccgatc aactggcgcc ctgcgctccg gtaccgatcg
     2221 atacacgaga acaaagaggt cttcagttcc tacggcaaac ggttcgagtt cctcgtcaag
     2281 accactagtg aagggatgaa ggaaccgtcg agcggcgaaa aactgaaaga ggagacgcag
     2341 ggacggcaga tacagtgggc aggcatcaac tacggttttt tcctctacgc ggcggtcgga
     2401 aaagaacaag aggcgctaac ggtcgacggc gtcatagact cgaagcagaa tctcacggtg
     2461 ctcaccgcag cgcacgacga gttcaccatc gcccccggcc agacggcgga agcggtcttc
     2521 accgtattcc tcggccctaa ggaggtaagg ctcctcgaac aggcgggcaa cgatctgcgc
     2581 gcctcggcat cgctcggctg gttcggcttc ctcgccgagc cactcctgtg gatactcaac
     2641 ttcttctacg actttgtgaa gaactacggt atcgcgatca tcctgctgac catccttgtg
     2701 aaactcctcc tctggccgct ctccgacgca tcgtacaagt cgatggccaa gatgaagaaa
     2761 ctgatgccga aggtagagga actcaagaag aagtacaagg aggacaagga gacgctcaac
     2821 aaggagatca tgctcctcta ccagaaggag ggggtcaatc cgctcggcgg ctgtctcccg
     2881 atactgatac agatgcccat ctacatcgcg ctctactcca tgctcaacaa cgccgtcgaa
     2941 ctctacaacg ccgggttcct gccgttctgg ctcgtcgacc tgtcggaaaa ggatccctac
     3001 tacatcctgc cggtcgcgct cggcggcttc atgttcctcc agcagaagat gacgccgacc
     3061 cagatggaca accagcaggc gaagatcatg ctctatgtga tgccggtgat gttcgccggg
     3121 ttcatgctct tccttcccgc cggactcaac ctctacatcc tcgccaacac actcctcggc
     3181 atcctccagc agtggctggt cacgaagcgg tacggctgat cgaggacgcg cggcgcggca
     3241 ccttgctttc cggcccatat cgattagaat gcgaccgtca ctttagcggg aggtccccct
     3301 tgagactatc gctcggcctt ttgtcgttgc tggctctttt tacgattgcc gcctgcgaga
     3361 atgatgtcca ttccctcgcc ccgtggagcg ccgattcata ttacggcgac cccgagggct
     3421 acgactggga taccgacgcg gcggggatgt gggaccggcg cccgaacctc gcggcctgct
     3481 cgaacgagga tgtgaacctc tggatactcg acaaactctg ggacatgtat ctgtggtacg
     3541 accatctcgc cgacgacatc gactttcgcg tcgaacttga gccgggcgac ttcctcggcg
     3601 ccgtcaagta caaggaatcg gatcatttca gttatctctc gaagaaggag gaccacgacg
     3661 actactacgg cggtcggtac tacggcttcg ggttcagttt ccgcaagttc cccgactatg
     3721 tcgtgtatgt gacgctcgtc atccccggca gcaacgccga gacggcgggg atgcgccgcg
     3781 gccagcgcat catcgcgata gacggcaaga cggtgcagga gatcgaggac aacaacctgt
     3841 ggagcacggt gacctcgcgg cccgaggata agaaggacat cccgctcgag tatgtcgtcg
     3901 aagaggaggg tgaggaaaag acgctcacgc tctacgcggg cgatgtgacc gcgccgtcga
     3961 tttacaagac ggacatcctc gatgacgacg accggaaggt gggctatctc atgctcaaat
     4021 cgttcattaa ctcgaccaac ggcgaactcg acaccgcctt caccgcgttc aaggcggcgg
     4081 gggtgaccga tctggtcgtc gacctgcgct acaacggcgg cggctggctc gacggttcgg
     4141 agcatctcgc gagtctcatc accggcggcg aactcaaaga cgaactgttc acgcggctca
     4201 cctacaacaa gaaacatcag gactacaaca gcgactacca cttcaaggac ttcgctcaga
     4261 gtctcggcct gcagaaggtc gtcttcatcg cgaccggcgg caccgcgtcg gcctccgagg
     4321 tggtcatcaa cgggctcaag ccctatgaca aggtggcgat cgtcggcggc aagacctacg
     4381 gtaaaccggt cgggatgaac ccgatgagtt actgcgacta cacgctggtg ccgataacct
     4441 tcaaactggc caacgccgac gggtacggcg actacttcac cggcatgccg gccgactgcg
     4501 aggccgacga cgatgtgacg cacgatttcg gcgacgcgcg ggagacctcg ctcgccgcgg
     4561 ccctttccta tctccgcgac ggcacctgcc ccgccgcccc ggcggtctat gcgtggaacc
     4621 ccgacgagat agatgtccgg acggtcccct accggctcgg ctggctgttc tgacgataat
     4681 tcccgaaaaa gatcagttcg gcgtgaaggt cagcatatcg ccgccgacct ccatgagcgt
     4741 gacgatctcg ttatcccggg cgtactgcgt gatggtcaca tcgccggaaa gatcgcccat
     4801 aacgatattc ccggtcttgg tccagcgggc gtagttgttg gtgccatcga gcgagaatgt
     4861 cttcggtatc gaccagccga tcgaataggc gcgtagctgg aactctatct tgctcccgtc
     4921 atcggtcgtg ccgtagaagg tggtgcggaa ataaacgccg ccggtgaagg gggttcgcgt
     4981 catgaggtcg gcggtcggga actcgaccgt tgcgtcgccg ttcacgacga cgaccttggc
     5041 ctccggtgca ttcatcgtgc tctgatcata gtagaaagag agcgtcgaga atttctcttc
     5101 cttgcatacc ccctcgtaca tgaccgagaa ccccgcctga ttggccaaac attcattgtc
     5161 ataggtcacc atgtcacagc cgcagaccgg cgagtagata tcgccgcaca tcgccggtat
     5221 ctcggtgcag gtcccgggtc ccgtgcagac gcccgtctcg tacaggcaga actggggatc
     5281 catccccgaa ccgtcattgc actcttcgtt cgagtagcaa ccggggttcg tcccgcattc
     5341 ccccgcgtgg tcgacattga cccccgcggc atgggcatcg caatcgctgc tgtaggtgtt
     5401 accgtcacag ccgcagaccg gcgcatagag cggcgggcac atctggggcc gctcctcgca
     5461 ggtgccggcg aggaatgtca tgcaggtgcc gtcaggtttg gcgcagtatt cggtctcttc
     5521 acaattctcg ttactgccac aggaattcac catatcgtcg tcaggccatt cgccatcgga
     5581 ccaatcgggc cactcactgt cggagccgtc gggccactcg ccatcggacc agtcgggcca
     5641 ttcgccgtcc gaaccgtcgg gccattcacc gtcggaccag tcgggccatt cgtcatccga
     5701 accgtcgggc cactcgccgt ccgaaccgtc gggccactcg ccgtccgaac cgtcgggcca
     5761 ctcgccatcc gaaccgtcgg gccattcg
//
LOCUS       DAOQUE010000132         5775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15328_length_5775_cov_4.171853, whole genome shotgun sequence.
ACCESSION   DAOQUE010000132 DAOQUE010000000
VERSION     DAOQUE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15328_length_5775_cov_4.171853"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="PKH10_05410"
     CDS             complement(<1..144)
                     /locus_tag="PKH10_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490348.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNT27605.1"
                     /translation="MSEKKIRVLIAKPGLDGHDRGAKFVARALRDAGMEVIYTGLRQT
                     PEMI"
     gene            complement(156..1805)
                     /locus_tag="PKH10_05415"
     CDS             complement(156..1805)
                     /locus_tag="PKH10_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNT27606.1"
                     /translation="MSISEKHAKWRDGAYKKTIEKFPERKKEFRSSWGDMVEPVYVPG
                     DTCPNYEKLGFPGEYPFTRGVQPTMYRGKFWTMRQYAGFGTAEESNKRYKYLLEQGQT
                     GLSVAFDLPTQIGYDSDHAMSDGEVGKVGVAIDSLRDMETLFNGIPLGKVSTSMTINA
                     TASVLLSMYIAVAEKQGFKPDELDGTIQNDILKEYIARGTYRFPPKPSMRIITDIFDF
                     CSKSVPKWNTISISGYHIREAGSNAVQEVAFTLADGLAYVDAAIKTGLPVDTFAPRLS
                     FFFNAHNDLLEEVAKFRAARRLWARLMKERFNAGPKSQMLRFHTQTAGCTLTAQQPDN
                     NIVRVAIQTLAAVLGGTQSLHTNSRDEALALPTEESVRIALRTQQIVAYESGVADTVD
                     PLAGSYYIESMTDRIEEKAAAYIKRIDELGGMVAAIEKGYPQQEIQDAAYRYQKEIES
                     TERIVVGTNKFQIKEQPPKNLLKVDRSIQVAQIKKIEAIKAERDNDLVKRRLAEVKKA
                     AGGTDNLMPKILDAVKAYATLGEIVGEMVGVYGEHKESVVL"
     gene            1978..2673
                     /gene="cmk"
                     /locus_tag="PKH10_05420"
     CDS             1978..2673
                     /gene="cmk"
                     /locus_tag="PKH10_05420"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007069786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNT27607.1"
                     /translation="MTKLRIAIDGPAGAGKSSVSKLIAERLGLSYIDTGALYRCVAFE
                     MIMNDVALDDERGIRRILADLKITFKMEKGRNLVFLNGREVTAQIRTEQVGMLASTAS
                     ALPFVREALFEMQRRYAEKGGVVMEGRDIGTVIMPEADLKIFLTARPESRADRRQKDI
                     RAAGREDKPYEELLEEIKRRDDQDTQREACPLRPAEDAVLLDNSGMTLEETAAHIGEL
                     AEKKMGPGACRRS"
     gene            2658..3548
                     /gene="ispH"
                     /locus_tag="PKH10_05425"
     CDS             2658..3548
                     /gene="ispH"
                     /locus_tag="PKH10_05425"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNT27608.1"
                     /translation="MPKVVTATYAGVCFGVKRAVDILDKALETGREEGRPVLMFGPLI
                     HNPRFIESYRDRGVEIVDRSGIRDHAVIVIRSHGITRDEEEYLKTFSDLRIIDTTCPF
                     VQRIHRLVAERSRDGWAIVVLGDAAHAEVEGFVSRIDGPYFVHPPDFSDPALVALDRF
                     LHGHGKFFAVAQTTSQPDHFRRLLERMGSACREARCRFEYQNTVCDATFQRQETAREL
                     AHAMDAMVVIGGRNSSNTAKLFAIVSAENPRSFWIEAPADLTAADRETLRGVEAIGLT
                     AGASTPDDQIAELASFLEAL"
     gene            3545..4423
                     /locus_tag="PKH10_05430"
     CDS             3545..4423
                     /locus_tag="PKH10_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNT27609.1"
                     /translation="MIVLRADNPLHPDHFLRREPPIGLPLWMEGMLERADARAVAVVG
                     TRKPSLYGIELARNIAAAAARHGITVVSGLARGIDTEAHRAALNAGGRTIAVLGTGLD
                     NLYPEENIALAADIAKSGALLSQFEPGTRPLPRHFPLRNRTIALLADATIIVEAGERS
                     GSLVTGRHAIEAGRTVHVAGGEVDAKGFRGSYEFLKKNQGDPRVRLLSSLDDLFGALP
                     SPDAPPAAPAPIPDDLSPAARAVVEFLREHGEDAAFDEIVEHTGLPAHDLPALLLPLV
                     IRGVVAEMPGNRYTCH"
     gene            4440..>5775
                     /gene="topA"
                     /locus_tag="PKH10_05435"
     CDS             4440..>5775
                     /gene="topA"
                     /locus_tag="PKH10_05435"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNT27610.1"
                     /translation="MKLIVVESPAKAKTLSRYLKGFRVLASMGHIRDLPEDDFGVDVD
                     NEFSPTYVTIKGKEKTVKMIQKEAAGADEIYLGSDPDREGEAIAFHIAHFLKEKEKPI
                     HRVLFHEITKSGVLKAIDEKIELNEDLFNAQQARRILDRIVGYRLSPFLWKSVKKGLS
                     AGRVQSVALKFIVDREREIAAFVPEEYWNIKGLFRRDGGDTVDIALDKVQGQKPKMGN
                     EREALAIEKRIREATGWKIESIEQKRRKENPLPPFNTSALQQEASRVYHFTADRIMRV
                     AQSLYEGKEIGAEGPFGLITYMRTDSFRVSPEANARLREFVTKEYGDTYLCPFTRTYE
                     NKKGKIQDAHEAIRPTDVSYTPEKVKPYLTKEELNIYTLIWRRFVATQMKEAEFDQTV
                     VTVSDASKDLSFRKTGSVMVFDGYRKIWERGNGREETLPAFTENEPLALAEVS"
BASE COUNT         1081 a         1878 c         1771 g         1045 t
ORIGIN      
        1 aatcatctcc ggggtctgcc gcaggccggt gtagatgacc tccattccgg cgtcgcgcag
       61 cgcccgcgcc acgaatttgg cgccgcggtc gtgcccatcg agccccggtt tcgcgatgag
      121 tacccgtatc ttcttttcgc tcattgccgc ctcccttaca gtacgaccga ttctttgtgt
      181 tcgccgtaga ccccgaccat ctcgccgact atctcgccga gcgtcgcgta ggccttgacc
      241 gcgtcgagta tcttcggcat caggttgtcg gtgccgcccg ccgccttttt cacctcggcc
      301 agacgccgct tgacgaggtc gttgtcgcgt tccgccttta tcgcctctat cttctttatt
      361 tgcgccacct gtatcgagcg gtcgaccttg aggaggttct tgggcggctg ttcctttatc
      421 tggaacttgt tggtgccgac cacgatccgt tcggtcgatt cgatctcttt ctggtagcgg
      481 taggccgcat cctgtatctc ctgctgggga tagcccttct cgatggccgc gaccatgccg
      541 cccagttcgt cgatccgctt gatgtaggcg gccgccttct cctcgatgcg atcggtcatc
      601 gactcgatgt agtaggaacc ggcgagcggg tcgaccgtgt cggcaacgcc gctctcgtag
      661 gcgactatct gctgggtgcg cagcgcgatg cggacgctct cctcggtcgg gagcgccagc
      721 gcctcgtcgc ggctgttggt gtggagcgac tgggtgccgc cgagtaccgc cgcgagcgtc
      781 tggatggcga cgcggacgat gttgttgtcg ggctgctgcg cggtgagcgt gcagccggcc
      841 gtctgagtat ggaaccggag catctgcgac ttgggaccgg cgttgaagcg ttccttcatc
      901 aggcgcgccc agagccgccg cgcggcgcgg aacttcgcga cctcttccaa gagatcgttg
      961 tgggcgttga agaagaacga caggcgcggc gcgaaggtat cgaccgggag ccccgtcttt
     1021 atcgcggcgt cgacataggc aagcccgtcg gcgagcgtga aggcgacctc ctgcaccgcg
     1081 ttgctgcccg cctcgcggat gtggtagccc gatatcgaga tggtgttcca cttcgggacc
     1141 gacttcgagc agaaatcgaa gatgtcggtg atgatccgca tcgacggttt cggcgggaag
     1201 cggtaggtgc cgcgggcgat gtattccttg aggatgtcgt tctgtatcgt gccgtcgagt
     1261 tcgtcgggtt tgaagccctg tttctcggcc acggcgatgt acatcgacaa aagcaccgaa
     1321 gcggtcgcgt tgatggtcat cgaggtcgag accttcccca gcgggatgcc gttaaaaagc
     1381 gtttccatgt cgcgcaggga gtctatcgcg acccccacct tccccacttc gccgtccgac
     1441 atcgcgtggt ccgagtcgta gccgatctgc gtcggcaggt cgaaggcgac cgaaaggccc
     1501 gtctgcccct gttcgagcag gtatttgtag cgtttattcg attcctcggc ggtcccgaac
     1561 ccggcgtatt gacgcatcgt ccagaatttg ccgcggtaca tcgtcggctg gacgccgcgg
     1621 gtgaagggat attcgccggg aaaaccgagt ttttcgtagt tcggacaggt gtcgccgggg
     1681 acatagaccg gttccaccat atcgccccac gaactgcgga actccttttt ccgttcgggg
     1741 aacttctcga tggtcttctt gtaggcaccg tcgcgccatt tggcgtgttt ctcgctgatc
     1801 gacatcgcta tctccgtatg aaagtgaacg gccataccgg ggcgagtcta gaccggccgg
     1861 agacaaaaat caaaaaaata accttactat gtaaggtttc ctttttcctt ccttctatct
     1921 cttgatttcc cttatatgac ggctatactg cccccgattt ttttgacggg ggtcgccatg
     1981 accaaactgc gcatcgcgat agacggcccg gcgggtgccg gcaaaagttc ggtcagtaaa
     2041 ctgatcgccg aacgactggg gctttcctac atcgataccg gcgcactgta ccgctgcgtc
     2101 gccttcgaga tgatcatgaa cgatgtcgcg ctcgacgacg agcggggcat ccgccggata
     2161 ctcgccgatc tcaagatcac cttcaagatg gagaaaggca ggaatctcgt gttcctcaac
     2221 ggccgcgagg tcaccgccca gatacggacc gagcaggtcg gcatgctcgc ctcgaccgcg
     2281 agcgccctcc ccttcgttcg cgaggcgctg ttcgagatgc agcggcgata cgccgagaag
     2341 ggcggcgtcg tgatggaggg gcgcgacatc gggaccgtca tcatgcccga ggccgatctc
     2401 aagatattcc tcaccgcgag acccgagagc cgcgccgacc ggcgccagaa ggacatccgg
     2461 gcggcgggca gggaggataa gccgtacgaa gaactgctcg aagagataaa aaggcgcgac
     2521 gatcaggaca cgcaacgcga ggcatgcccg ctccgccccg ccgaagacgc ggtattgctc
     2581 gacaattcgg gcatgacgct cgaggaaacg gcggcacaca tcggcgaact ggccgaaaag
     2641 aagatggggc ccggcgcatg ccgaaggtcg tgaccgccac ctacgccggc gtctgcttcg
     2701 gcgtcaagcg ggccgtcgac atcctcgaca aggcgctgga gaccggccgc gaggaaggac
     2761 gcccggtgct catgttcggg ccactcatcc acaacccccg attcatcgaa agttatcgcg
     2821 accgcggggt cgagatcgtc gaccggtccg gcattcgcga ccacgcggtc atcgtgatac
     2881 ggtcgcacgg catcacccgc gacgaagagg aatatctgaa gaccttctcc gacctgcgca
     2941 tcatcgacac cacctgtccc ttcgtccagc ggatccaccg gctcgtcgcc gaacggtcgc
     3001 gcgacggctg ggccatcgtg gtgctcggcg acgcggcgca tgccgaggtc gagggcttcg
     3061 tgtcgcgcat cgacgggcca tacttcgtcc atccgcccga cttctccgat cccgcgctcg
     3121 tggcgctcga ccggttcctg cacggtcacg ggaaattctt cgcggtcgcc cagacgacga
     3181 gtcagcccga ccatttccgg cgactgctcg aacggatggg aagcgcctgt cgcgaggcgc
     3241 ggtgccggtt cgaataccag aacaccgtct gcgacgccac cttccagcgg caggaaacgg
     3301 cccgcgaact ggcccacgcg atggacgcga tggtggtgat cggcggccgc aacagctcga
     3361 acaccgccaa actcttcgcc atcgtcagcg ccgagaaccc ccgttccttc tggatcgaag
     3421 cacccgccga cctgaccgcc gccgaccgcg agaccctgcg cggtgtcgag gcgatcgggc
     3481 tgaccgcggg cgcatcgacc cccgacgacc agatagccga acttgcctct tttctggagg
     3541 cgttgtgata gtcctgcgcg ccgacaatcc cctgcacccc gaccacttcc tgcgccggga
     3601 gccgcccatc ggtctcccgc tctggatgga ggggatgctg gagcgggccg acgcccgcgc
     3661 cgtcgcggtt gtcggcactc gtaaaccgtc cctgtacggc atagaactgg cgcgcaacat
     3721 cgcggcggcg gccgcgcggc acggcatcac agtggtgagc gggctcgccc gcggcatcga
     3781 caccgaggcg caccgcgccg cgctgaacgc cggtggccgc accatcgcgg tgctcggcac
     3841 cggtctggac aacctctatc ccgaggagaa tatcgcgctc gccgccgaca tcgcgaagag
     3901 cggcgcgctc ctgtcgcagt tcgagccggg tacccggccg ctcccgcgcc attttccgct
     3961 tcgcaaccgc accatcgcgc tcctcgccga cgccacgatc atcgtcgagg cgggcgaacg
     4021 gagcggcagt ctggtgaccg gccgccacgc catcgaggcg ggccgcacgg tccatgtcgc
     4081 cggcggcgag gtggacgcga agggcttccg cggcagttac gagttcctga aaaagaatca
     4141 gggcgatcct cgggtgcgac tgctctcttc gctcgacgac ctgttcggcg ccctgccgtc
     4201 gcccgacgcg ccgcccgccg ctccggcacc gatacccgat gatctttccc ccgccgcacg
     4261 cgccgtcgtg gagttcctcc gggaacacgg tgaggacgcg gccttcgatg agatagtgga
     4321 acacaccgga cttccggcgc acgaccttcc cgcactcctg ctcccgctgg tgatacgggg
     4381 cgtcgtcgcc gagatgccgg gcaatcgata cacctgtcac tgaagcgagg tacccagaga
     4441 tgaaacttat cgtcgttgaa agtccggcca aggccaaaac gctctcccgc tacctgaagg
     4501 gcttccgcgt gctcgcctcc atggggcaca tccgcgacct gcccgaggac gatttcgggg
     4561 tcgatgtgga caacgagttc tccccgacct atgtcaccat caagggtaag gagaagacgg
     4621 tcaagatgat ccagaaggag gcggcgggcg ccgatgagat atacctcggc tccgaccctg
     4681 accgcgaagg cgaggccatc gcgttccaca tcgcccattt cctcaaggag aaggaaaagc
     4741 ccatacaccg cgtcctgttc catgagatca ccaaaagcgg cgtgctcaag gccatcgacg
     4801 agaagataga actcaacgaa gacctcttca acgcccagca ggcgcgacgg atactcgatc
     4861 gcatcgtcgg ctaccggctg tcgccgttcc tgtggaaatc ggtcaaaaag ggactctcgg
     4921 cgggccgcgt ccagtcggtc gcgctcaagt tcatcgttga ccgcgaacgg gagatagccg
     4981 ccttcgtccc cgaggaatac tggaacatca aggggctgtt ccgccgggat ggcggcgata
     5041 cggtcgacat cgcgctcgac aaggtgcagg gccagaaacc gaagatgggg aacgaacgcg
     5101 aggcgctcgc gatcgagaaa cgtatccgtg aggcgaccgg ctggaagatc gagtctatcg
     5161 agcagaaacg gcgcaaggag aatccgctcc cgcccttcaa cacctcggcg ctccaacagg
     5221 aggcatctcg agtgtaccac ttcacggccg accgcatcat gcgggtcgcc caatcgctct
     5281 atgagggcaa ggagatcggc gcggaaggcc cgttcggcct catcacctat atgcgcaccg
     5341 actcgttccg cgtgtcgccg gaggcgaacg cgagactgcg cgaattcgtc acgaaggaat
     5401 acggggacac ctacctctgc cccttcaccc gcacctacga gaacaagaaa gggaagatac
     5461 aggacgccca cgaggcgata cgccccaccg atgtctccta tacccccgaa aaggtcaaac
     5521 cctacctgac caaagaagaa ctcaacatct acaccctcat ctggcggcgg ttcgtcgcga
     5581 cccagatgaa ggaggccgag ttcgaccaga cggtcgtcac cgtcagcgac gcatcgaagg
     5641 acctgtcgtt ccgcaagacc ggctcggtca tggtgttcga cggctatcgc aagatatggg
     5701 aacggggcaa cggccgcgag gagacgctcc cggcgttcac cgagaacgag ccgctcgcgc
     5761 tcgccgaagt atcca
//
LOCUS       DAOQUE010000133         5767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15346_length_5767_cov_3.858718, whole genome shotgun sequence.
ACCESSION   DAOQUE010000133 DAOQUE010000000
VERSION     DAOQUE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15346_length_5767_cov_3.858718"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="PKH10_05440"
     CDS             <1..1198
                     /locus_tag="PKH10_05440"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNT27611.1"
                     /translation="IPSVLSLLRKPQPVIEVDYRKRHLLERFMYGVAFLISKNPKQVL
                     VVTLLAALVAALFIPRLTGDSNLARYYPAGSTVARSSEIVNEQFGGGQIVNVVVEGDI
                     KDPSILRKIDAFEKEMEKLPEVGKSTSIVRLIKMISRNLHKPGEPGYDAIPDSRGAVD
                     FYLTEYAKMADIQEIAHLVTADFRHAQIMVQINDEGSGTIKRVVAKVNELTAGDPAFR
                     IVGGSGYIFAALIDEVSKGQVTSLILSVIIVAILVMLLFRSFMAGFVASIPLALALVF
                     LFGIMGGAGIPLDIATAMLSSIMIGVGVDYTIHFLWRYREELRIGKSPEKAMRRTLRT
                     TGRGIVFNAVSVMLGFSVLILSVFMPIRFFGVLIVLAISTCLLGALFIMPAYCLVFRP
                     KFLEKR"
     gene            1210..2343
                     /locus_tag="PKH10_05445"
     CDS             1210..2343
                     /locus_tag="PKH10_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27612.1"
                     /translation="MARNAFLIFFAVLLCSAPLLAQENDEQVFYFFYSRSCPHCKEAH
                     PFVEELKKRHPELTFRDLEVSQSEENRALFKRKCDELKIESRGVPTFILGAKSVVGFR
                     KEVHDKMIEQMIHEHLNSEQTCGDRTDKLIHVPVFGTIDPQLVSLPSFTFILGLLDGI
                     NPCAMWVLMFLLTLLVNAGSRRKLIMIGSVFVASSAIVYFLFMTAWLNIFMFLGIKEY
                     VTIALGLVAVLMGLINMKEFFFFKKGVSLMIPESAKPKLFDKMRKVMNENDLWLAFAG
                     TVALAFFVNLIELACTIGFPAIYTRILSIQNIGTLPKYLYMALYNIYYVIPLGAIVLL
                     FVLTMGKYRFQEKHAKVLKLVSGSLMLALGLILVFKPDLLVFS"
     gene            complement(2340..3434)
                     /locus_tag="PKH10_05450"
     CDS             complement(2340..3434)
                     /locus_tag="PKH10_05450"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNT27613.1"
                     /translation="MRIDPLIAADIPLAFDFPDAAPDPGRTALIALGKSALFMVTRFF
                     LRHPSFRAVPGMAIVPEGLPRDDADLPSGLTVIASTHPHITGKSFAAGEALVEFVSAQ
                     AADRDTFIVLLSGGASALIEYGSDPAEFMRLNRELLRAGLPIQEMNRRRIALSGIKGG
                     KLPRFAPQADWTTFVMSDIPAPDGHRLVGSMPSWSPDEPRHHLVPVANGGLLHDRYAA
                     ELRRNNYRIAAEERFLTGTVGDWTRHIAAQELGPGEALLITGEPTLALTIATPGIGGR
                     MAHLALLLAERLDEGLTLHALSSDGIDGASPYAGVIFTPEDRSRFDRTLIADHLARFD
                     AYSFFEKYGCALKTGYTGVNLNDVVIVTRD"
     gene            complement(3437..5434)
                     /gene="priA"
                     /locus_tag="PKH10_05455"
     CDS             complement(3437..5434)
                     /gene="priA"
                     /locus_tag="PKH10_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNT27614.1"
                     /translation="MNTADRFVRLVIPRPGIEELYYRLEGDMKAGPGSLVEIDLRNET
                     IWAIVREVVAALPDELRDLACRPVRRVIGDTPLFHDPRETRFLQWLGDYYLYPFPKLV
                     KQIVAPFISPRRTLPRPSAPVPPCDTPPCTAMLDDDQRRVATAIRGRWRADDRRPVLL
                     FGVTGSGKSEVFADLCREVFDAGGQVLYLVPEVGLTPGTIAHLESRLGERAVIAHSYL
                     SPKKRFDAFSRACRGEAKLVVGTRSALLYPLSKVGLIIVDEEHDPSYKNMEPPYYHAR
                     DAAVMKASLLDIPVILGSATPSSESWHNAERGKYHLERLPRRANRRPLPAVRIFPYRG
                     DTYLPSELLDAVREGVARREQALFFVNRRGFSTIAVCRSCEAIQKCPRCEVALVYHKK
                     KGRLLCHHCNFSIAPDRCAACTGTDLSLEGIGIERFTETLRGLFPGASVVSIDRDSVP
                     DETALSTELAKIEDGTHDIVAGTVMISKGHNFPALRWVVIKHADFLLSLADYRAAERC
                     FQVVAQVAGRAGRFDIPGEVWAEAQKPEHYLWRHLPEYDFEGFIDEELAWRRALLLPP
                     FSRLAVVKVVASSEKGADETAKALYEELRGMTAGKKVRLLPPAAPPLQRVHNRYRRHI
                     VLSAASLRPLAPLLADLISRHGRRRGVTVTCDMDALSTVQV"
     gene            complement(5421..>5767)
                     /locus_tag="PKH10_05460"
     CDS             complement(5421..>5767)
                     /locus_tag="PKH10_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27615.1"
                     /translation="LFFLPSVAKDADPAEPLMAHLAKLGVNTEKKVLSTTGPGGELIL
                     IIGKERADESAPHLALYRSGLLPFRLSYGETTVTFGAYHKSVLPLAFPGIVEIREPAR
                     TVRYRFLRNEYR"
BASE COUNT         1039 a         1715 c         1929 g         1084 t
ORIGIN      
        1 catcccgtcg gtcctgtcgc tcctcaggaa gccgcagccg gtcatcgagg tcgactaccg
       61 caagcgccac ctgctcgaac ggttcatgta cggcgtcgct tttctcatat cgaagaaccc
      121 gaaacaggtg ttggtcgtga ccctgctcgc ggcgctcgtc gcggcgttgt tcatcccgcg
      181 cctcaccggc gattcgaatc tcgcccgtta ctacccggcc ggttcgacgg tggcgcgctc
      241 atcggagatc gtcaacgagc agttcggcgg cggccagatc gtcaatgtgg tggtcgaggg
      301 ggacatcaag gatccctcga tactgcgtaa gatagacgct ttcgagaaag agatggagaa
      361 attgcccgag gtcggcaaga gcacctccat cgtccggctc ataaagatga tatcgcgtaa
      421 cctgcacaag ccgggcgagc cggggtacga cgccataccc gacagccgcg gcgcggtcga
      481 tttctacctc accgagtacg cgaagatggc tgacatacag gagatagccc atctggtcac
      541 cgccgacttc cggcatgccc agatcatggt gcagataaac gacgagggct ccggcacgat
      601 aaaacgggtg gtcgccaagg tgaacgaact gacggcgggc gatcccgcct tcaggatcgt
      661 cggcgggtcg gggtacatat tcgcggcgct catcgacgag gtgtcgaaag gtcaggtgac
      721 ctcgctcatc ctgtcggtga tcatcgtcgc gatactggtg atgttgctgt tcaggtcgtt
      781 catggcggga ttcgtcgcct cgataccgct cgccctcgcg ctcgtgttcc tgttcggcat
      841 catgggcggc gccggcatac cgctcgacat cgccaccgcg atgctttcct ccatcatgat
      901 cggcgtaggg gtcgattaca cgatacattt cctctggcga taccgcgagg aactgcggat
      961 cggcaagtcg cccgagaagg cgatgcgccg gacgctccgc accaccggtc gcggcatcgt
     1021 gttcaacgcc gtgtcggtca tgctcggttt ctcggtgctg atcctgtcgg tgttcatgcc
     1081 gatacggttc ttcggcgtgc tcatcgtgct ggccatatcg acctgtctgc tcggcgcgct
     1141 gttcatcatg ccggcctact gtctcgtgtt caggccgaag ttcctcgaga agagataagg
     1201 aggtccgtca tggcgcgaaa cgcattcctg atattcttcg cggtgctttt gtgctcggcc
     1261 ccgcttctgg cgcaggagaa cgacgagcag gtgttctatt tcttctattc gcggtcgtgt
     1321 ccccattgca aggaggccca tcctttcgtc gaggaactca aaaaacgcca tccggaattg
     1381 accttccgcg atctggaggt gtctcagagc gaagagaacc gcgcgctgtt caagcgcaag
     1441 tgcgacgagc tcaagataga gagtcgtggc gtcccgacct tcatcctcgg cgcgaagtcg
     1501 gtggtggggt tccggaaaga ggtgcacgac aagatgatcg agcagatgat acacgaacat
     1561 ctgaacagcg aacagacctg cggcgaccgg accgacaaac tcatccatgt acccgttttc
     1621 ggcacgatag acccgcaact cgtcagcctg ccctccttca ccttcatcct cggcctgttg
     1681 gacggtatca acccctgcgc gatgtgggtg ctcatgttcc tgctcaccct gctggtcaat
     1741 gccggtagcc gccggaaact catcatgata gggagcgtct ttgtcgcctc gtcggccatc
     1801 gtctatttcc tcttcatgac cgcgtggctt aacatcttca tgttcctcgg catcaaggag
     1861 tatgtgacga tagcgctggg gctcgtcgcg gtcctcatgg gactcatcaa catgaaggag
     1921 ttcttcttct tcaagaaagg ggtcagtctc atgataccgg aaagcgccaa gccgaaactc
     1981 ttcgacaaga tgcgcaaggt catgaacgag aacgaccttt ggctcgcctt tgccggcacc
     2041 gtcgcgctcg cctttttcgt caatctcatc gaactcgcct gcacgatcgg gttccccgcc
     2101 atctatacga ggatcctttc catccagaac atcggtaccc tgccgaagta cctctacatg
     2161 gcgctctaca acatctacta tgtcatcccg ctcggtgcca tcgtgctcct gttcgtgctg
     2221 acgatgggca aataccgttt tcaggaaaag cacgcgaagg tgttgaaact ggtgtcgggc
     2281 tcactcatgc tggcgcttgg tctgatactg gtgttcaagc ccgacctgct ggtgttctcc
     2341 tagtcccgcg tcactatcac gacatcgttc agattgacac cggtgtagcc ggtcttcagc
     2401 gcacagccgt acttttcaaa aaaagaatag gcgtcgaacc gtgcaaggtg gtcggcgatg
     2461 agcgtccggt cgaaccggga ccggtcctcc ggcgtgaaga tgacgccggc atacggagag
     2521 gcgccgtcga tgccgtcgct tgaaagtgcg tggagcgtca gcccctcgtc gagccgctcg
     2581 gcgagcagaa gggcgaggtg agccatccga ccgccgatgc ccggcgtcgc gatggtgagg
     2641 gcgagcgtcg gctcgccggt gatgagaagc gcctcgcccg ggccgagttc ctgcgcggcg
     2701 atgtgccggg tccaatcgcc gaccgtgccg gtgaggaacc gctcctcggc ggcgatgcgg
     2761 tagttgttcc gtcgcagttc ggcggcgtag cggtcgtgca ggaggccgcc gttggcgacc
     2821 ggcacgagat ggtggcgcgg ctcatcgggt gaccacgagg gcatcgagcc gacaagacgg
     2881 tgtccgtcgg gggcgggaat gtcgctcatc acgaaggtgg tccagtcggc ctgcggggcg
     2941 aagcggggca gtttgcctcc cttgatgccc gacagcgcga tgcgccgccg gttcatctcc
     3001 tgtatcggga gtccggcacg gagcagttcg cggttgagcc gcatgaattc ggcgggatcg
     3061 gaaccgtatt cgatgagcgc cgaggcgccg cccgacagca ggacgatgaa ggtgtcgcgg
     3121 tcggcggcct gcgccgagac gaactcgacg agcgcctcgc cggcggcgaa actctttccg
     3181 gtgatgtgcg gatgggtcga ggcgatgacc gtgagccccg aaggcaggtc ggcatcgtcg
     3241 cgaggcagac cttccggtac gatcgccatg ccgggaaccg ctcggaagga ggggtgtcgc
     3301 aggaagaagc gtgtcaccat gaagagggcc gatttgccga gcgcgatgag cgcggtgcgc
     3361 cccgggtcgg gggccgcatc gggaaagtcg aaggcgagcg gtatgtcggc ggcgatgagc
     3421 ggatcgatgc gcatcgctac acctgcaccg tggaaagggc gtccatgtcg caggtgacgg
     3481 tgacgccgcg ccggcggccg tgtcgggaaa taagatcggc aaggagcggc gcgagtgggc
     3541 gcagactcgc cgccgaaagg acgatatggc ggcggtagcg gttgtggacc cgttggagcg
     3601 gcggcgcggc gggcggcagg agacgcacct tctttccggc ggtcatgccg cgcagttcct
     3661 catacagcgc ctttgccgtt tcgtcggccc ccttttcgga ggaggcgacc accttgacga
     3721 ccgccagccg cgagaaggga ggcaacagga gcgcgcgtcg ccaggcgagt tcctcatcga
     3781 tgaagccctc gaaatcgtac tcgggaaggt ggcgccagag gtaatgttcc ggcttctgcg
     3841 cctcggccca cacctcgccg ggtatgtcga accttccggc gcgtcccgcc acctgtgcca
     3901 cgacctgaaa gcaccgttcc gcggcgcggt aatcggcgag cgacagcagg aagtcggcgt
     3961 gcttgatgac cacccaccgg agcgcgggga agttgtgtcc cttgctgatc atgacggtcc
     4021 cggcgacgat atcgtgggtc ccgtcctcta tcttcgcgag ttcggtcgaa agggcggtct
     4081 cgtcggggac gctgtcacga tctatcgaaa cgaccgaggc gccggggaaa agtccgcgga
     4141 gcgtttcggt gaaccgttcg atgccgatgc cttcgagcga aagatcggtc cccgtgcagg
     4201 cggcgcaccg gtcgggggcg atcgagaagt tgcagtggtg gcagagcagg cggcctttct
     4261 ttttgtgata gacgagcgcg acctcacagc gcgggcattt ctggatcgcc tcgcaggaac
     4321 ggcagaccgc gatggtcgag aagccgcgcc ggttgacgaa aaaaagcgcc tgttcgcggc
     4381 gagcgacccc ttcacgcacc gcgtcgagca gttcggaagg cagataggtg tcgccgcggt
     4441 aggggaagat gcggacagcg ggaagcggtc gccggttggc gcgtcgggga agccgctcga
     4501 ggtggtattt gccccgctcg gcgttgtgcc acgattcgga cgatggtgtc gcgctcccga
     4561 ggatgaccgg tatgtcgagc aggctcgcct tcatcaccgc agcgtcgcgg gcgtgatagt
     4621 agggcggctc catgttcttg taactggggt cgtgctcctc atccacgatg atgagcccga
     4681 ccttcgagag cgggtaaaga agcgccgagc gggtcccgac gacgagtttc gcctcgccgc
     4741 ggcaggcgcg ggaaaaggcg tcgaagcgtt ttttgggact caggtaactg tgcgcgatga
     4801 ccgcccgctc gccgagccgc gactcgaggt gcgctatcgt gccgggggtg agtcccacct
     4861 ccggcacgag gtacagcacc tgtccgccgg cgtcgaacac ctcgcgacac agatcggcga
     4921 agacctcgct cttgccgctg ccggtgacgc cgaagagaag caccgggcgc cggtcgtcgg
     4981 cgcgccaccg tccgcggatg gccgtcgcga cgcgccgttg atcgtcgtcg agcatcgccg
     5041 tacacggggg agtgtcgcac ggcgggaccg gggcggaggg gcgcggcagg gtgcggcgcg
     5101 gcgatatgaa cggggcgacg atctgcttga cgagtttggg gaacgggtag agatagtagt
     5161 cgccgagcca ctggaggaac cttgtttcgc gcggatcgtg gaacagaggg gtgtcgccga
     5221 tgacgcggcg caccgggcgg caggcgaggt cgcgcagttc atccggcagt gcggcgacga
     5281 cctccctcac gatggcccag atggtctcgt tgcgcaggtc tatctcgacg agagaaccgg
     5341 gtccggcctt catgtcgccc tcgagccggt agtagagttc ctcgatgccg gggcgcggta
     5401 tgacgagccg gacgaagcgg tcagcggtat tcattgcgga ggaaacggta ccggaccgtt
     5461 cgcgccggtt cccgtatctc gacgatgccg gggaaggcga gcggcaggac gcttttgtgg
     5521 tatgcgccga aggtgaccgt cgtttcgccg tacgaaagcc ggaaggggag caggccgctt
     5581 cggtaaagag cgaggtgggg cgcactctcg tcggcccgct ccttgccgat gatcaggata
     5641 agttcgccgc cgggtccggt ggtcgagagc accttcttct cggtgttcac gcccagtttc
     5701 gcgagatgcg ccatgagcgg ctcggccggg tcggcatcct tggcgaccga cggcaggaaa
     5761 aagaggc
//
LOCUS       DAOQUE010000134         5766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15348_length_5766_cov_3.747505, whole genome shotgun sequence.
ACCESSION   DAOQUE010000134 DAOQUE010000000
VERSION     DAOQUE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15348_length_5766_cov_3.747505"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKH10_05465"
     CDS             complement(<1..194)
                     /locus_tag="PKH10_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27616.1"
                     /translation="MRNALLTVPFLFFLSLYAADIREPARAGQWYSADPQALRAQVAG
                     LFAQVPFDSTAKGLQPFIIVA"
     gene            complement(228..947)
                     /locus_tag="PKH10_05470"
     CDS             complement(228..947)
                     /locus_tag="PKH10_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT27617.1"
                     /translation="MTRYGIIILAVVAILCGPGCGMKSPEDERRLAEKAFKDALSGKD
                     CAREDFLAAEELLMKAREAVNAKRYDEAKKLFLSAKEKAEKIAAYVRSHPDECDPKVK
                     KEKVAQRTAGVDDGAISEETLSSKDPNMKFPVIHFEFNEYAVREEDKPLLKIMAEWMV
                     NFPAAAIRVEGHADERGSIDYNMSLGERRAQEVRDELMKLGVDAKRFQIVSYGEERPI
                     ASGQNEDAWFQNRRAEFVRVN"
     gene            complement(967..2280)
                     /gene="tolB"
                     /locus_tag="PKH10_05475"
     CDS             complement(967..2280)
                     /gene="tolB"
                     /locus_tag="PKH10_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNT27618.1"
                     /translation="MGLHFKYAVMFALLTALCGGTAHAQLKGEVRDGALVAYAIAAPA
                     FVHSGDTTGELARALTETLRRDLDLTGYFQVLDERSFIEAPTVPVDKVNFKNWLTVGA
                     KGLVKATLTGTDPIELEMQAFDVAGGKSILHKKYKASAKAIRKAPHGFVRDLVQALAG
                     IRLKFLSSRIAFVEKEAKTYKLMVADFDGADATAVVTSDKILTLPGWSADGTHLFYTA
                     YTTGEPHLYAVDIKSRKVSLVSDHPGLNTSASAAPDGKNIALRLSKDGNAEIYLMNLA
                     SKALTRLTSNIAIDTAPSFSPDGKEIAFVSNRSGNPHIYRLFVNDPARVERLTDQGKY
                     NQDPDYAPDGRYIAFTGRDEFFMFDIFLFDLQARTISRVTQKQGKNENPSFSPDGALM
                     VFSSDRTGKNGIWVTNLAGDRQRKIYGGTGEAITPSWSPEVTITQ"
     gene            complement(2253..3011)
                     /locus_tag="PKH10_05480"
     CDS             complement(2253..3011)
                     /locus_tag="PKH10_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNT27619.1"
                     /translation="MRSYIFAAPVFEAEKRHMKRFAAVSLLLHVGVAFAVMTIGAYLN
                     MAPKIAFESVETRLVKFGEKPRDKKLLPRVVKKEEVAAPKVGAANALKEPEKKKEPEK
                     SKEEPKKATDLNALLGEALKDIKKDARAEETKEGSPDGVRDGDVTDPAFAVKGSLYVR
                     QISALIRRNWKIPSLITPDLLATLKAEIYFRITPSGEVYDLQVVTPSGDNNFDSSVIE
                     AVKLTAKLPLPEDRQLKKYVLTEGLQWGFTSSTL"
     gene            complement(3008..3430)
                     /locus_tag="PKH10_05485"
     CDS             complement(3008..3430)
                     /locus_tag="PKH10_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNT27620.1"
                     /translation="MSMGVNGGKKRRPIAEINITPMVDIMLVLLIIFMVAAPMIEQGV
                     DLDLPQTETIPIENPKDKFILRIFKNEKIFIEKTEIPLKELETKLKANAKLQQDRELY
                     LYASQRLSYGYVVRVMAIVKKAGVPKINLVTDPLEEEG"
     gene            complement(3430..4158)
                     /locus_tag="PKH10_05490"
     CDS             complement(3430..4158)
                     /locus_tag="PKH10_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT27621.1"
                     /translation="MQMTSFLLDAIGNAATAISIVEVVKNSSLVVNLVLGGLVLLSVL
                     CWGVIIFKFIQHRRAIVATEQFIELFWKNMDFQEVYRRSDKLQDSPIATVFRAGYSEF
                     FETKKSWESKGITVVGSKVDELVANIERAMKRESQTRNQLLENSLTLLATTASSAPFI
                     GLFGTVWGIMDAFHIIGKTGNATLGTIAPAISEALIATAFGLAAAIPAAICYNYFTSN
                     IQKIEGESANITSDFLNSIKRSSV"
     gene            complement(4282..5076)
                     /locus_tag="PKH10_05495"
     CDS             complement(4282..5076)
                     /locus_tag="PKH10_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27622.1"
                     /translation="MTASRAVFFLFALSFLLSCASVPDTHQVLFPRGELPEKRLTLLA
                     PVLLNELKGVEDPSEVYDRNLYVGLIRFSGGFIEDVGRGMMARSRELRFSHQNRFSLV
                     IRESFAGMVRNILDARGTPYNLSDADPTPLLAVTERSLIDRYPEEGHDNTNLPRIAYD
                     IVPRTPFPADAALTGRLVVLPVVEFYYGHNGGWFNGQTFGCPAGIRFRVHVLIVDGDS
                     GEMFYRFSDEERYLVPKQYRMNIIEIEQYLLDARRALFDKLLRHFP"
     gene            complement(5118..5570)
                     /locus_tag="PKH10_05500"
     CDS             complement(5118..5570)
                     /locus_tag="PKH10_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27623.1"
                     /translation="MKKVTLLVALTFAAIMLSGCTTAYQAISQQYLTPLPVDYEIIGT
                     TMAESERTQVFWVEWAYLFHDEQADITNRGSFNAFLGGIIEDTKAAALYAALEKMPDA
                     DKLIEPRYKIEVTNFLIGKTVHVTVWAKAIKYLKSSPYIVSPAMAAAQ"
BASE COUNT         1098 a         1703 c         1715 g         1250 t
ORIGIN      
        1 gcgacgatga taaagggctg gagtcctttc gccgtgctgt cgaacggcac ctgcgcgaac
       61 agtcccgcca cctgcgcccg gagcgcctgc gggtcggcgg aataccactg cccggcgcgg
      121 gccggttcac ggatatcggc agcgtacagg gacaaaaaga agagaaaggg aacggtcagc
      181 agggcgttcc tcatggatca cctccctccc gaggtctcgc gataccgtca attgacgcgg
      241 acgaactcgg cgcgtctgtt ctggaaccac gcatcctcgt tctgccccga ggcgatgggc
      301 cgctcctctc cgtatgaaac gatctggaac cgtttggcgt ccacgcccaa tttcatcagt
      361 tcgtcgcgta cctcctgggc ccgccgctcg ccgagcgaca tgttgtaatc gatgctcccg
      421 cgttcgtcgg cgtgcccctc gacgcggatg gccgccgccg ggaagttcac catccattcg
      481 gccatgatct tgaggagcgg cttatcttcc tcgcgcaccg cgtactcatt gaactcgaaa
      541 tgaatgacgg ggaacttcat gttgggatcc ttcgacgaaa gcgtctcctc ggagatcgcc
      601 ccgtcatcca cgcccgccgt ccgctgggcg accttttcct ttttcacctt cgggtcgcat
      661 tcgtcggggt gggaccggac atacgcggcg atcttttcgg ccttttcctt ggcggaaagg
      721 aaaagtttct tcgcctcgtc gtacctctta gcattgaccg cctcgcgcgc cttcatcagc
      781 aattcctccg ccgcgaggaa atcctcgcgg gcgcagtcct tgcccgaaag cgcgtctttg
      841 aacgcctttt cggcgagtcg ccgttcgtcc tcgggcgact tcatgccaca gccggggccg
      901 cacagtatcg cgacgaccgc cagaatgatg atgccgtacc gggtcatatt cctcctcctt
      961 cggttattac tgggtgatcg tcacttcagg cgaccacgac ggcgtgatcg cctcgcccgt
     1021 tccgccgtat atctttctct ggcggtcccc ggcgagattg gtgacccaga tgccgttctt
     1081 ccccgtccgg tcggaagaga aaaccatgag cgcgccgtcg ggcgagaacg acgggttctc
     1141 gttcttccct tgtttctgtg tcacgcgcga tatggtccgc gcctgcaggt cgaacaggaa
     1201 gatgtcgaac atgaagaact cgtcgcgccc cgtaaaggcg atgtatcgac catcaggcgc
     1261 gtagtctgga tcctgattgt atttaccctg atcggtcaga cgctccaccc gtgccggatc
     1321 gttcacgaac aggcggtaaa tgtgcggatt gcccgaacgg ttcgatacga aggcgatctc
     1381 cttgccgtcg gggctgaaag atggcgcggt atctatcgcg atgttgctcg taagccgcgt
     1441 gagcgccttg gacgcgaggt tcatgaggta gatctcggca ttgccgtcct tggaaagacg
     1501 cagtgctatg ttcttgccgt cgggcgcggc cgaagcggag gtgttgagcc ccggatggtc
     1561 ggaaacgagc gagaccttgc ggcttttgat gtccaccgca tagaggtgag gttccccggt
     1621 ggtgtaggcg gtgtagaaca ggtgcgtccc gtcggccgac cagccgggaa gcgtgagtat
     1681 cttgtcgctc gtcacgaccg ccgtcgcgtc ggcgccatcg aagtcggcga ccatcagttt
     1741 gtaggtcttc gcctctttct ctacgaaggc aatgcggctc gaaaggaatt tgagccgtat
     1801 accggccagc gcctgcacga gatcgcgcac aaagccgtgc ggcgccttgc ggatggcctt
     1861 cgccgacgct ttgtacttct tgtggaggat gcttttaccg cccgccacat cgaatgcctg
     1921 catctcgagt tcgatcgggt cggtcccggt gagggtcgcc ttgacgagac ccttcgcgcc
     1981 gaccgtgagc cagttcttga agttcacctt atcgaccggc accgtgggcg cctcgatgaa
     2041 cgaccgctcg tcgagcacct gaaagtagcc ggtcaggtcc agatcgcggc gcagggtctc
     2101 ggtgagggcg cgcgccagtt ccccggtggt gtcaccacta tggacgaagg ccggcgcggc
     2161 gatggcgtac gcgacgagcg ccccgtcgcg cacctcgccc ttgagttgcg cgtgagcggt
     2221 ccccccgcac agcgccgtca gcaacgcgaa cattacagcg tacttgaagt gaagccccat
     2281 tggagtccct ccgtgagcac atatttctta agttgcctgt cttccgggag cggcagtttc
     2341 gccgtcagtt tcaccgcctc tataaccgaa ctgtcgaaat tgttgtcgcc tgacggcgtc
     2401 acgacctgca ggtcgtacac ctcgcccgac ggcgtgatgc ggaagtatat ctcggccttg
     2461 agcgtggcga gcagatcggg cgtgataagc gagggtatct tccagttgcg gcggatgagt
     2521 gccgatatct gccgcacata gagactgccc ttcaccgcga aggccgggtc ggtcacatca
     2581 ccgtcacgga caccgtcggg actcccctcc ttcgtttcct cggcgcgggc gtccttcttg
     2641 atgtccttca gtgcctcgcc gagcagggcg ttcaggtcgg tcgccttttt cggctcctct
     2701 ttgctttttt cgggctcctt ctttttctcc ggttctttca atgcgttcgc cgcaccgacc
     2761 ttcggagccg cgacctcctc ctttttcacg acacgcggca ggagtttttt gtcgcgcggc
     2821 ttttcgccga acttcacgag ccgcgtttcg accgattcga acgctatctt gggcgccatg
     2881 ttgagatagg cgccgatcgt catgacggca aaggcgacac cgacatggag caggagcgac
     2941 acggcggcga accgcttcat gtgccgcttc tcggcctcga agacgggcgc cgcgaagata
     3001 taggacctca tccctcctcc tcgagcggat cggtgacgag atttatcttg gggacccccg
     3061 ctttcttgac tatcgccatc acgcggacca catagccgta ggaaagccgc tggctcgcgt
     3121 agaggtacaa ctcgcggtcc tgctggagtt tcgcgttcgc cttgagtttc gtctcgagtt
     3181 ccttgagcgg tatctcggtc ttctctatga atatcttctc gttcttgaag atgcgcagga
     3241 tgaacttgtc cttcgggttc tctatcggga tggtctcggt ctgcggcagg tcgagatcga
     3301 ccccctgttc tatcatcggc gccgcgacca tgaagatgat gaggagcacg agcatgatgt
     3361 ctaccatcgg ggtgatattt atctcggcga tcggtcgacg cttcttgccg ccgttcactc
     3421 ccatgctcat cagaccgaac tccgctttat cgagttgagg aagtcgctcg tgatgttcgc
     3481 cgactcgccc tctatcttct gtatgttgct cgtgaagtag ttgtagcata tggctgccgg
     3541 tatcgccgcg gcgagcccga aggcggtcgc gatgagcgcc tccgatatcg ccggcgcgat
     3601 ggtgccgagc gtcgcgttgc cggtcttgcc gatgatgtgg aatgcgtcca tgatgcccca
     3661 cacggtgccg aacagcccga tgaagggggc cgaactcgcc gtcgtcgcaa ggagcgtaag
     3721 cgagttctcg agcaattggt tgcgggtctg cgactcgcgc ttcatggcgc gctcgatgtt
     3781 ggcgaccagt tcgtcaacct tcgacccgac gacggtgatg cccttcgact cccacgactt
     3841 cttggtctcg aagaactccg aatagccggc gcgaaacacg gtggcgatgg gtgaatcctg
     3901 cagtttgtcg ctccggcggt agacctcttg gaaatccatg ttcttccaga aaagttctat
     3961 gaactgttcg gtggcgacta tcgcgcggcg gtgctggatg aacttgaata tgatgacgcc
     4021 ccagcagagg accgacagca ggacgagccc gccgaggacg aggtttacca cgagcgatga
     4081 attcttcacc acctcgacga tcgagatggc ggtcgcggcg ttaccgatcg cgtcaaggag
     4141 gaacgaggtc atctgcatct ggaggctcct tcatgattac tttctcttac tcgtcatcgg
     4201 aaatgaacat aatacaaact ggcgggggag tcaaaaatat tccgttattt cgcaggaata
     4261 agcataaaga ccggtgcggt cctacggaaa atggcgaagc agtttatcga acagtgctct
     4321 tcgtgcatcg agcaggtact gttctatctc tatgatgttc atgcggtatt gtttcggcac
     4381 gaggtaccgt tcctcgtcgc tgaaccggta gaacatctcg ccgctgtcgc cgtcaacgat
     4441 gagcacgtgg acgcggaacc gtatccccgc cggacagccg aatgtctgac cgttgaacca
     4501 accgccgttg tgcccgtagt agaactcgac caccggcagt accaccagtc gccccgtgag
     4561 cgccgcatcg gccgggaacg gcgtccgcgg aacgatatcg taagcgatgc gcggaaggtt
     4621 ggtgttgtcg tgtccctctt cggggtaacg atctatcaac gagcgttcgg tcaccgcgag
     4681 caggggcgtc gggtcggcgt ccgacagatt gtacggggtc ccgcgggcat cgaggatatt
     4741 cctcaccata ccggcgaaag actccctgat gaccaacgag aacctgtttt ggtgactgaa
     4801 acgaagttcg cgcgatcggg ccatcatgcc gcgaccgaca tcctcgatga agccgccgga
     4861 gaaccggatg agaccgacat agagattgcg gtcgtagacc tccgacgggt cctcgacgcc
     4921 cttcagttca ttcaagagga cgggagcgag gagcgtcagc cgtttttcgg ggagttcgcc
     4981 ccgcgggaac agtacctgat gggtatccgg caccgacgcg cacgaaagaa gaaacgacag
     5041 agcaaagagg aaaaagaccg cacgcgatgc cgtcatgacg agctcaacat gtgaccgtca
     5101 aagaaaccaa gaggcggtca ctgcgcggcc gccatggcgg gagaaacgat gtagggagat
     5161 gacttaagat acttgatcgc cttggcccag acggtaacat gcacggtctt gccgatgagg
     5221 aagttggtga cctcgatctt gtagcggggc tcgatcagtt tgtcggcatc gggcatcttt
     5281 tcgagcgccg cgtagagcgc cgccgccttg gtgtcctcaa tgatgccgcc gaggaaggca
     5341 ttgaaggagc cacggttagt gatgtcggcc tgttcgtcgt ggaacaggta ggcccactcg
     5401 acccagaaga cctgagttct ttccgattcg gccatcgtgg tgccgatgat ctcataatcg
     5461 accggaagcg gggtcaggta ctgctgcgat atcgcctgat aggcggtggt gcagccggaa
     5521 agcatgatcg cggcaaaggt cagcgcgacg agcaaggtca cttttttcat agggatcctc
     5581 cttactaaaa aacaaatttc acatgacaat ccagacaacg gacgaccgtt acattaaaaa
     5641 aacgaccctg tcaatttttc ttggcgcccc gcttgacgca acggacaccg gcgagatcgc
     5701 cagtgttctt gctgaatatg taggccgaat agaagtgtat ggcccaggcg cggcgcatcg
     5761 cgtcgc
//
LOCUS       DAOQUE010000135         5763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15363_length_5763_cov_3.399089, whole genome shotgun sequence.
ACCESSION   DAOQUE010000135 DAOQUE010000000
VERSION     DAOQUE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15363_length_5763_cov_3.399089"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKH10_05505"
     CDS             complement(<1..257)
                     /locus_tag="PKH10_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27624.1"
                     /translation="MKIGRILFPAIAFVLAACASTPQEKLAEGDRYLTVNKEKARGYY
                     QSACDEGSMVGCHKAGLAWLSGDAPSPENEKGRALIDRACQG"
     gene            complement(361..1497)
                     /locus_tag="PKH10_05510"
     CDS             complement(361..1497)
                     /locus_tag="PKH10_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT27625.1"
                     /translation="MPRRRFLIFILLLCAALPAAAAKKGGGQGNEKLLFAVQAMDVSR
                     AQAAVKAGANINGADEDGWPFFITAVTSGSTEMINFFIARKVKLDLKGPDGKTALMHA
                     ISSGNTELALTLLQKGAGRETTDNTGKTPLMYAAEKGNVKVTQLLIKKKADPYKTDKE
                     GRNALAHAVENRQREVIKILKVFEISPDELATAVIKGDTVAARKLILKGANVNAPNYE
                     GRPPIMIAIEQNDKTMVRLLLDSKANPSIKNAKGHSLLMFCFASGKFDLAKMLLRYKA
                     SADLDFQYGKGRTALMIAIQRNDRELIRMLLERDMNLNIQDEDGNTALMLAVEKNMST
                     IIKDMLKKGADPDIKRGDGLYAELIAKARGYTYIGRLLRESSGK"
     gene            complement(1497..2909)
                     /locus_tag="PKH10_05515"
     CDS             complement(1497..2909)
                     /locus_tag="PKH10_05515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT27626.1"
                     /translation="MNDTASFGNTHAPDRVPEHLRGYNDWTIGAMTDLVNFAAITLEP
                     APLETIPSQVVSFLARPLFDLSFIGLFRFDAATAQWTVLASHGTGDIDLDEVPNFEGN
                     TLLEEDREIYSFFSILDEAFLVLVTRAPDRPVFSPSEYSFMSLFSTLFSSFYQMKKLA
                     KQVEEKIVEMSNLSASSKLIAGLRENTVTVEEVFLELADTLSLDAFALALCDETVEKP
                     HVVLARGIDEKDWDTLLAEIYTDEKHFPDEWEMFSLFDENLVLFGAFICRLAKQNPTL
                     YAIQRRVLDHTVAQVTTILSQRQFHTEALTDPLTGIANRRNIMKVLKERIRAARLDPL
                     SKLSVAMIDIDHFKKVNDTYGHPAGDAVLKAVAQAVASALRGTDLAARYGGEEFLVVM
                     QAGRKGAYHVSERIRRSIAKLRVKAGDHTLSVTASVGYAVCTGLTLSPEEIIARADAF
                     LYQAKEAGRDRTFGDRPEEA"
     gene            3072..>5763
                     /locus_tag="PKH10_05520"
     CDS             3072..>5763
                     /locus_tag="PKH10_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT27627.1"
                     /translation="MKRTLLALLILALAGCAATVHRTPVASVAAAPAPGTAPFCIPAV
                     RPTGPEAALLAEIFFGVGPRVGDIDRLLDAALAKYPDAYELHEMAAWWQTMLGDDRRA
                     VHHYMKAACDLASPRTDIYLHALRDALSTDEKRRFLPVLAVLSVEHPDPVLRPIAAAM
                     WSEILLDLGRVEEARAVTKGIGYITDWSVIGSFDNDQGKGFYEAYPPETEIDLAKRYK
                     GKVGEIGWMPVLPTRHDGLLSLQNLLYPGSWSTAYLLTHVRVDAETDARVVISTGNPT
                     AVWLNGAAVHANDRIDSFFYDTVSVPVRLAKGWNRLLVKSAAENRGWLFAARLTDATG
                     APLRGAIFSRTVADAALATPVAQPAPPAPEEELLPSIAQQNRQAFLQALSDQRNGLRK
                     KGRQAFISLYEGNGQNPLAMFFAAQGLIENEEEGRLIDLLNRALSPQGMPTAGFLAIR
                     GDFYRRKGLRDSAEDDYKAARALDPAAPAAYWGLNGLFRDRRWHKDRRDIVKAMRDAW
                     PDDADIVREEADCAEALGYFDEAERILVAAVALEPGRVAFLRKLATMARMRHDLPAAL
                     RWVGTLARLAPDNPAYPTEQAELLRRMKRYDEARKFLDHAAALAPSSPGAYQELGDLA
                     WEQGRREEALTQWKKALDRNPKNSSLAERVTYLSVGEKDIAERYLPSEAEMNARIAAA
                     KAVTPHPGAQAIIALDKAAVHINDDGSSIWYITDVKLVVNDAGRDMLISESLPFMGRL
                     KILKAYSLDPQGMRQEASSIRGGQIRFRQLDKGSVTVVQYIHYSPPPHFLDNHFLGQW
                     YFQTVSQQMLYSELRLIHNPKRELVFDIRAPVEERIETVEGLTMRTFTAVDRPPLMLE
                     PMAPPVSDFLDQMTISSIKGWEDYVNWERALLKNVFASTRE"
BASE COUNT         1003 a         1736 c         1893 g         1131 t
ORIGIN      
        1 ccctgacagg cgcggtcgat gagggcgcgc cccttttcgt tctcgggact cggcgcgtcg
       61 cccgaaagcc acgcgaggcc ggccttgtgg cagcccacca tcgagccctc gtcgcaggcg
      121 ctctgatagt agccgcgcgc cttttccttg ttgaccgtca ggtaacgatc gccttccgcc
      181 agtttctcct gcggcgtcga ggcgcaggcg gcgagcacga aggcgatggc agggaacagg
      241 atgcgtccga tcttcatggg tgaccccctc ggttgtcttc tttccacaaa cgaacggtga
      301 cagcatagcc ggaaagatat aaaaagcaaa atagcgtcgt tggaaagcgt ctctgtcgcg
      361 tcatttgccc gaactctccc gcaggagccg tccgatgtag gtgtacccgc gcgccttggc
      421 gatgagttcg gcgtatagtc cgtcaccccg ttttatgtcg ggatcggcgc cttttttcag
      481 catgtccttg atgatggtgc tcatgttctt ctcgacggcg agcatgagcg cggtgttgcc
      541 gtcctcatcc tggatgttca ggttcatgtc ccgttcaagg agcatccgga tgagttcgcg
      601 atcgttgcgc tgtatcgcta tcatgagggc ggtgcgtccc ttgccgtact ggaaatcgag
      661 gtcggcggac gccttgtagc gcaggagcat cttggcgaga tcgaacttgc cggaggcgaa
      721 gcagaacatg aggagcgagt gtcctttggc gttcttgatg ctcgggttcg ccttgctgtc
      781 cagcaggagt cgcaccatcg tcttgtcgtt ctgttcgatg gcgatcatga tgggcggacg
      841 tccctcgtag ttcggcgcgt tgacattcgc tcccttgagt atcagtttgc gcgccgcgac
      901 ggtgtcgccc tttatgacgg cggtcgccag ttcatcgggc gatatctcga acaccttgag
      961 tatcttgatg acctcgcgct ggcggttctc gacggcgtgc gccagtgcgt tgcgcccctc
     1021 cttgtcggtc ttgtaggggt cggccttctt cttgatgagc agttgcgtca ctttgacatt
     1081 gcctttctcg gcggcgtaca tgagcggggt cttgccggtg ttgtcggtgg tctcgcgccc
     1141 ggcccctttc tgcaggagcg tcagggcgag ttcggtgttc cccgacgata tggcgtgcat
     1201 gagcgccgtt ttgccgtcgg gccccttgag gtcgagtttc accttgcgcg ctatgaagaa
     1261 gtttatcatc tcggtggagc cgctcgtcac cgccgtgatg aaaaagggcc agccgtcctc
     1321 gtcggcgccg ttgatgttcg ctcccgcctt gaccgccgcc tgagcgcgcg agacatccat
     1381 cgcctgcacc gcgaagagca gtttttcgtt accctgaccg ccacctttct tcgcggcggc
     1441 ggcggggagc gcagcgcaca gcagaaggat aaagatgaga aaccgtcgcc ggggcatcat
     1501 gcctcctccg ggcggtcgcc gaaggtccgg tcgcgacccg cttccttcgc ctggtagaga
     1561 aatgcgtcgg cgcgggcgat gatctcctcg ggagacagcg tgagcccggt gcagacggca
     1621 tagccgaccg acgcggtgac cgagagtgtg tggtcgccgg ccttgacgcg cagtttcgct
     1681 atgctgcggc gtatccgttc cgatacatgg taggcgccct tccggcccgc ctgcatcacg
     1741 acgaggaact cttcgccgcc gtatcttgca gcgaggtcag tgccgcggag cgccgaggcg
     1801 acggcctgcg cgacagcctt gagtaccgcg tcgcccgccg gatgcccgta ggtgtcgttg
     1861 accttcttga aatggtcgat atctatcatc gcgacggaaa gtttggagag cgggtcaagc
     1921 cgcgcggcgc ggatgcgctc cttgagcacc ttcatgatgt tgcggcggtt ggcgatgccg
     1981 gtgagcgggt cggtgagcgc ctcggtgtgg aactgtcgct gggagaggat ggtggtgacc
     2041 tgcgcgacgg tgtggtcgag cacgcggcgc tgtatcgcgt agagcgtcgg attctgtttg
     2101 gcgaggcggc atatgaaagc gccgaaaagc acgaggttct cgtcgaacag agagaacatc
     2161 tcccactcgt cggggaaatg cttctcgtcg gtgtatatct cggcgagcag agtgtcccag
     2221 tccttctcgt cgatgccgcg cgcgaggacc acgtgaggct tttcgaccgt ttcgtcgcag
     2281 agcgcgagcg cgaaggcgtc gagcgagagc gtgtcggcga gttcgaggaa cacctcctcg
     2341 acggtgacgg tgttctcgcg aaggccggcg ataagtttcg aggaggcgga cagattggac
     2401 atctcgacta tcttctcctc gacctgcttg gcgagtttct tcatctggta gaacgaagag
     2461 aacagggtcg agaaaagcga cataaaagag tattcggagg gggagaacac ggggcgatcg
     2521 ggcgcgcgcg ttacgagcac gaggaatgcc tcgtcgagta tggagaagaa cgagtatatc
     2581 tcgcggtcct cctccagaag cgtattgcct tcgaagttcg gcacctcatc gagatcgatg
     2641 tcgccggtgc cgtgggaggc caggaccgtc cactgcgcgg tcgccgcgtc aaaacggaac
     2701 agtccgatga atgagagatc gaagaggggg cgggcgagga acgacacgac ctgtgacggt
     2761 atcgtctcca gcggcgccgg ctcgagcgtg atggcggcga aattgaccag atcggtcatc
     2821 gcgccgatgg tccagtcgtt gtagccgcgg aggtgttccg ggacacggtc gggagcgtgc
     2881 gtgttgccga aggaggcggt gtcgttcatg gcgtctcggc cgttgtttcg gggcactata
     2941 gcaaaaaggc gggcccattg caattttaga ggtacctaaa aaaactgccg cagggcggga
     3001 aaacaaaatt tgcgtgatat ggcgccgcgt gttatggtgt cgcgacatcc gttctctgag
     3061 agaggtgacg catgaaaagg accttgcttg cccttctgat cctcgcgctc gccggttgtg
     3121 ccgctaccgt tcaccggacc ccggtggcgt ccgtcgcggc ggcccccgcg cccgggaccg
     3181 ctcccttttg catacccgcc gtacgaccga ccggtcccga agcggctttg ctcgccgaga
     3241 tattcttcgg ggtgggaccg cgcgtcggcg atatcgacag gttgctcgac gcggcgctcg
     3301 ccaagtatcc ggacgcctat gaactgcacg agatggcggc gtggtggcag accatgctcg
     3361 gcgacgatcg tcgcgcggtc catcactaca tgaaggcggc ctgcgacctt gcgagccccc
     3421 gcaccgacat ctatctgcat gctcttcgcg acgccctgtc caccgatgag aagcggcggt
     3481 tcctgccggt gctggcggtc ctgtcggtcg aacaccccga ccccgttctc cgcccgatcg
     3541 ccgcggcgat gtggagcgag atcctgctcg acctcggtcg cgtcgaagag gcgcgggcgg
     3601 tcacaaaggg catcggctac atcaccgact ggtcggtcat cggttcgttc gacaacgatc
     3661 agggcaaggg attctacgag gcctatccgc cggaaacgga gatcgatctg gcgaagagat
     3721 acaagggaaa ggtcggcgag atcggctgga tgccggtcct gccgacgcgt catgacggtc
     3781 tgctgtcgct tcagaacctt ttgtatccgg gcagttggtc caccgcctat cttctcaccc
     3841 atgtgagggt cgatgccgag accgacgcga gggtggtcat cagcacgggc aatccgaccg
     3901 ccgtctggct gaatggcgcg gcggtccatg ccaacgaccg gatcgattct tttttctacg
     3961 atacggtgag cgttccggtg cggctcgcca aaggatggaa ccgtcttctc gtcaaatcgg
     4021 ccgccgagaa ccgcggctgg ctcttcgcgg cgcggctcac cgacgcgacg ggcgccccgc
     4081 tgcgcggcgc gatcttttca cggaccgtcg ccgatgctgc gctcgcgacg ccggtcgctc
     4141 agccggcgcc gcccgcaccg gaggaggagc tgttgccgtc catcgcccag cagaatcgac
     4201 aggcgttcct gcaggcgctc tccgatcaac gcaacggcct gcggaaaaag gggcgacagg
     4261 ctttcatctc cttgtacgag gggaacgggc agaatccgct cgcgatgttc ttcgccgcgc
     4321 aggggctcat cgagaacgag gaggagggcc gcctgataga tcttctgaac cgtgccctgt
     4381 cgccgcaggg gatgccgacc gccggattcc tcgcgattcg cggcgatttc taccggcgca
     4441 aggggctccg cgacagcgcc gaggacgatt acaaggcggc gcgagccttg gatcccgcgg
     4501 ctcccgcggc ttactggggg ctgaacggtc ttttccgtga ccgtcgctgg cacaaggacc
     4561 ggcgcgatat cgtgaaggcg atgcgcgacg catggcccga cgacgccgat atcgtccggg
     4621 aagaggccga ctgcgccgag gcgctcggtt atttcgatga ggcggaacgg atcctcgtcg
     4681 cggcggtcgc gctcgaaccg gggcgggtgg cattcctccg caaacttgcc acgatggccc
     4741 gcatgcggca cgatcttccc gccgcgctcc ggtgggtggg gacgttggcc cggctggccc
     4801 ccgataaccc tgcctatccg accgaacagg ccgaactcct gcggcgcatg aaacggtacg
     4861 atgaggcccg gaaattcctc gatcatgccg ccgcgctcgc tccgtcgtcc cccggtgcct
     4921 atcaggaact cggtgatctg gcgtgggagc agggacggcg cgaggaggcg ctcacccagt
     4981 ggaagaaggc gctcgaccgt aacccgaaga actcgtcgct cgcggagcgg gtgacctacc
     5041 tgtcggtcgg cgagaaggac atcgccgaac gctacctgcc ctccgaggcc gagatgaatg
     5101 cccgtatcgc cgcggcgaag gcggtgaccc cgcatcccgg agcgcaggcg atcatcgcgc
     5161 tcgacaaggc ggcggtccac atcaacgacg acgggtcgag catctggtac atcaccgatg
     5221 tgaaactggt ggtcaacgac gcgggccgcg acatgctcat cagtgaatcg cttcccttca
     5281 tggggcggct gaagatactc aaggcctatt cgctcgaccc gcaggggatg cggcaggagg
     5341 cgtcgtccat ccggggcgga cagatacggt tccgtcaact cgacaaggga tccgtgacgg
     5401 tggtgcagta catccactac agcccgccgc cccatttcct cgacaaccat tttctcggcc
     5461 agtggtattt ccagaccgtt tcccagcaga tgctctactc ggaactgcgg ctcatccaca
     5521 atcccaagcg ggaactggtg ttcgacatcc gcgcgccggt cgaggaacgg atcgagacgg
     5581 tggaggggct gacgatgcgg accttcacgg cggtggaccg cccgccgctc atgctggagc
     5641 cgatggcgcc gccggtgtcc gattttctcg atcagatgac catcagttcc atcaagggct
     5701 gggaggacta tgtcaactgg gaacgggcgc tccttaagaa tgtgttcgct tcgacccggg
     5761 aaa
//
LOCUS       DAOQUE010000136         5713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15513_length_5713_cov_4.444503, whole genome shotgun sequence.
ACCESSION   DAOQUE010000136 DAOQUE010000000
VERSION     DAOQUE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15513_length_5713_cov_4.444503"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKH10_05525"
     CDS             complement(<1..302)
                     /locus_tag="PKH10_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27628.1"
                     /translation="MNTKMLESLLATVLTKDQIQALREEIVAGARRLHKVVDENERAR
                     ARRDELLALMKRIDTFIETREGSPLVLRAIYSAAKEEIRRRVMPLIEEVLRQGEEEG"
     gene            complement(304..906)
                     /locus_tag="PKH10_05530"
     CDS             complement(304..906)
                     /locus_tag="PKH10_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT27629.1"
                     /translation="MRSVKKESRREQILSCAARIFAKKGYHATSISDICKRSDVARGT
                     IYLYFKNKRDIFDTLIKNFTGDIFRRISVFTPGEALQPQFNRNMCAVVDIITQNKDLT
                     KIMVSEAVGLDAEFDHQLILFYARLVEYVEGALTMLQKNGDISKHVNVRLLAYALVGA
                     IKEIAYQWALDGGNVLDMDPLIKTARQFNIQNFIAFTGES"
     gene            complement(991..3420)
                     /locus_tag="PKH10_05535"
     CDS             complement(991..3420)
                     /locus_tag="PKH10_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27630.1"
                     /translation="MRTARDTLVFLSFAAPIVAASFLLGGVYTATTYNLIVMRIVACL
                     FFAWAVWLSVSGRRLFVPLLVVPLLLIMGWALLHLLPLPPAIVGLLSPTARYFHAMAG
                     PEGWRPLTMSSPDTLYSIMRTATLVLFILMLQRTIEVERRGWRRLVTDTIVVTAALLF
                     AFGVFLKLTGVETFLGSTLRGPLLMHPTFINLNHAAAFFGVASLLSLATAVEADLARA
                     RVFYGALFFVNLLAVVMTLSRGGILAVVCAMVFFFALKLFKERTRVLLLPVLFSLVAV
                     ATAFYVGYNLIVAEFDLSRPDYFDKFLLLTTVGDYLADFWRVGSGLGSFMHVYPYYQG
                     DPGLFFIQLENEPVQFALEHGLPFALIVAALIVWFFVKYNRREHCRCGAAAILLFILM
                     HNTLDFNLHDLAILFPVAAVYLMASGSFELKGWPRRLALGAGMLAALGLLWGSRYGDD
                     LDFYRAKDMLPYDTLVRRYPADFKVPLRDALARYNDGRRERFLAALPFLSEAAAKAPD
                     YYHISYLTGSALLRLGSIEEGILLYVKAVDRAPERHLGRLLGDIYRHLGAAGIEPRMR
                     ELLPFGRPGARETVSRFLASVRSPELVGSFIAGREEEFFETAGQLYLSTGDTARLTDL
                     LDRVSALAGDWPVARRGRYLMLRGQAALRRKDPATALSLFRQGAALTGAFDDVLTLAQ
                     ATLSHEPDRCGELDAMLQEKALTDKGRLARYHRWKSDWLWRRGEMRESLKYLQRGADI
                     GGYPQWRIDGARRLASQGLWEEAVAELTHLKDSRKQVDVAAVDALIEEYRRRMERKRS
                     GLMQEMLLEKR"
     gene            complement(3434..4954)
                     /locus_tag="PKH10_05540"
     CDS             complement(3434..4954)
                     /locus_tag="PKH10_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27631.1"
                     /translation="MLVLLCALPLGALTLDARQVKDPQIQEQLTRDATLVPMEKGAVF
                     VPWLVDSQREPTYAVYRGEEFITDAETGRRVVLDPGDYVLYIGSGPLDTRIVFYVTVK
                     PERVTVVMPVWGALVVQVVDAYRNPLREGYQISDEETKLYYGAGSGTDETLGEKPLIW
                     ILRPGLYRVSKKGEAPDSYRNFVTVRLIERHASTVLLVFDEKTHQLLGGGEVAGDQQM
                     TQKGNWDLRLLLSGSLSFTNSGYMIEGAIAENSLAIGSALNAAALYDDDDWLYSARLD
                     LFEDLMMQDNRKVRNTRDMAKFDTSVIYRIEKWVGPYLSTRLNTRLFFDYRDYSKTGL
                     TYVGVERDGRRWQMRPGDILIAKTGSPTTVQEGLGFNFEYRWGTLFFLTARAGYGLKQ
                     DIAPFYYDESHDDLATPEYEIVRIDPFNFSHGPEFSIYFTLMPLSFLEIKEDFISLLP
                     VERPEQTYFSAETTVSIWISSFATIQYLFWVERQPFISQDIRGYHAVTVQLFYRVF"
     gene            complement(4977..5576)
                     /locus_tag="PKH10_05545"
     CDS             complement(4977..5576)
                     /locus_tag="PKH10_05545"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNT27632.1"
                     /translation="MRRTAATLGLVFCAALLFGGCFGSQAKKHYYRAYYRPQTARVVP
                     VPATARVKTLEIDKVYRRYNLVYRTSPYEIFYYPNHYWASRPEDMMTDLVYAHLKVAG
                     LFTDLIVKLDKTPDYEVTGEIREIDMFDSGDRWFAHLSMVLTLKDFKTGAVLAAHPFD
                     ERREVFGREPVHTVRAIGEIAEQEIERFIQHIAAELGKK"
BASE COUNT         1079 a         1804 c         1828 g         1002 t
ORIGIN      
        1 ccctcctctt cgccctgccg caggacctcc tcgatgagcg gcatcactcg ccggcgtatc
       61 tcctccttcg ccgcgctgta gatggcgcgc aggaccagcg gcgacccttc gcgcgtctcg
      121 atgaaggtat cgatgcgctt catgagcgcg agcagttcgt cgcggcgggc acgggcccgc
      181 tcgttctcgt cgaccacttt gtgcaggcgg cgcgcgccgg ccacgatctc ctcgcggagc
      241 gcctgtatct ggtccttggt cagcaccgtc gcaagcaggc tttcgagcat cttcgtgttc
      301 atgtcaggat tctcccgtaa aagcgatgaa gttctgtatg ttgaactgcc gggccgtctt
      361 gatgagcggg tccatgtcga gcacattccc gccgtcgagc gcccactggt acgcgatctc
      421 cttgatcgcc ccgacgagcg cgtaggcgag gagccgcaca ttgacatgtt tcgatatgtc
      481 gccgttcttc tggagcatgg tgagcgcccc ctcgacatac tcgacgagcc gcgcgtagaa
      541 caggatgagt tgatggtcga actcggcgtc gagccccacc gcttccgaca ccatgatctt
      601 ggtcaggtcc ttgttctgcg tgatgatgtc gaccaccgcg cacatgttgc ggttgaactg
      661 cggctggagc gcctcgccgg gcgtgaagac cgatatgcgc cggaagatgt caccggtgaa
      721 gttctttatc agcgtgtcga atatgtcgcg cttgttcttg aaatagaggt atatggtgcc
      781 gcgtgccaca tcggaccttt tgcagatgtc gctgatgctc gtcgcgtgat accccttctt
      841 ggcgaatatc cgcgccgcgc aggagagtat ctgttcccgg cggctttcct ttttcaccga
      901 acgcatgacc gctccttccc tcttttcatg ccgcatatcg tatcggcgcc ggccgaaaag
      961 tcaaacttgc cgcgccgggt cgcccgtcgc ctatcgcttc tcaaggagca tctcctgcat
     1021 aagaccggac cgtttccgct ccatccggcg gcgatattcc tcaatgagcg catcgaccgc
     1081 ggcgacatcg acctgtttgc gggaatcctt caggtgggtc agttcggcca ccgcctcctc
     1141 ccagagcccc tgcgaggcga gccggcgtgc gccgtcgata cgccactggg gatagccgcc
     1201 gatgtcggcg ccgcgttgca ggtatttgag cgattcgcgc atctcgccgc gccgccacag
     1261 ccagtcgctc ttccagcggt ggtaccgggc gagccgtccc ttgtcggtga gcgccttttc
     1321 ctgcagcatc gcgtcgagtt ccccgcaccg gtccggctcg tgagagagcg tcgcctgagc
     1381 gagggtcagg acatcgtcga acgcgccggt cagcgccgcc ccctgccgga acagcgacag
     1441 cgccgtcgcc gggtccttcc tgcgaagcgc cgcctgcccg cggagcatga ggtaacgacc
     1501 ccgccgcgcc accggccagt cgccggccag cgcggagacg cggtcgagca gatcggtaag
     1561 ccgcgcggtg tcgccggtcg aaaggtacaa ctgcccggcc gtctcgaaga actcctcctc
     1621 gcggcccgcg atgaaggagc cgacgagttc cggggatcgc accgacgcga ggaaccggga
     1681 aacggtctcc cgcgcgccgg gccgaccgaa cggcagcaac tcacgcatcc gcggctcgat
     1741 gcccgccgcg ccgaggtgcc ggtagatatc gccgagcagg cgtccgaggt gccgttccgg
     1801 cgcccggtcc accgccttca catagagcaa gatgccctcc tcgatcgagc cgagccgcag
     1861 gagcgccgag ccggtgagat aggagatatg gtagtagtcg ggcgccttcg ccgccgcctc
     1921 ggacaggaac gggagcgccg cgaggaaccg ttcccgcctg ccgtcgttat agcgggcgag
     1981 cgcgtcgcgg agcggcacct tgaagtcggc cggatagcgc cggacgagcg tgtcgtacgg
     2041 gagcatatcc ttcgcccggt agaagtcgag gtcgtcaccg taccgcgagc cccacaggag
     2101 gccgagcgcc gcgagcatcc cggcgccgag cgccagacgg cgcggccagc ccttgagttc
     2161 gaacgagccg ctcgccatga gatacaccgc cgcgacgggg aacagtatcg cgagatcgtg
     2221 caggttgaag tcgagcgtgt tgtgcatgag gatgaacagc agtatcgccg ccgcgccgca
     2281 ccggcagtgt tcgcgccggt tgtatttgac gaaaaaccac acgatgagcg ccgcgacgat
     2341 gagcgcgaac ggcaggccgt gctcgagcgc gaactgcacc ggctcgttct cgagttggat
     2401 gaagaacagg ccgggatccc cctgatagta cgggtagaca tgcatgaaac tgccaagtcc
     2461 cgaaccgacg cgccagaaat cggcgagata gtcgccgacg gtcgtcagga gcaggaattt
     2521 atcgaaataa tcggggcgcg acaggtcgaa ctcggcgacg atgaggttat agccgacata
     2581 gaaggcggtc gcgacggcga cgagcgagaa gagcaccggc aggagaagca ctctggtccg
     2641 ttccttgaag agtttgagcg cgaagaaaaa gaccatggcg cagacgaccg ccagtatccc
     2701 gccgcgcgac agcgtcatga cgaccgcgag caggttgacg aaaaagagcg cgccgtaaaa
     2761 gacccgggcc cgggcgagat cggcctcgac ggcggtcgcg agcgacagga gcgacgcgac
     2821 gccgaagaag gccgcggcat ggttcaggtt gatgaaggtc gggtgcatga gaagcggccc
     2881 gcgcagggta gagccgagga aggtctcgac acccgtcagt ttgaggaaca cgccgaacgc
     2941 gaagagaagc gcggcggtca cgacgatcgt gtcggtcacg agccgccgcc acccgcgccg
     3001 ttccacctcg atcgtccgct ggagcatgag tatgaacagc acaagcgtcg ccgtccgcat
     3061 gatcgagtag agcgtgtcgg gcgacgacat cgtgagcgga cgccacccct ccggacccgc
     3121 catcgcgtgg aaatagcgcg ccgttggcga caacagaccg acgatcgcgg gcggcagtgg
     3181 caacaggtgg aggagcgccc accccatgat gagcaggagc ggcacgacaa gcagcggcac
     3241 gaacagccgt cgccccgaca ccgacagcca cacagcccac gcgaagaaga ggcacgcgac
     3301 gatccgcatg acgatgaggt tgtaggtcgt ggcggtgtag acgccgccga gcaggaacga
     3361 cgcggcgacg atcggcgccg cgaacgaaag gaagacgagc gtgtcccggg ccgtgcgcac
     3421 gatcgtcacc ccctcagaac acgcgataga acagttgcac ggtgacggcg tgatacccgc
     3481 ggatgtcctg cgatatgaat ggttgacgct cgacccagaa caggtactgg atggtcgcga
     3541 aactcgatat ccagatggat acggtggtct cggcgctgaa ataggtctgc tcgggccgtt
     3601 ccaccggcag gagcgatatg aagtcctcct ttatctcgag gaacgagagc ggcatgagcg
     3661 tgaagtagat cgagaactcg gggccgtggg agaaattgaa cgggtctatc cggacgatct
     3721 cgtactccgg cgtcgcgagg tcgtcatgcg attcgtcgta gtagaacggc gcgatgtcct
     3781 gcttgaggcc gtacccggcg cgggcggtca ggaagaagag cgtgccccag cggtactcga
     3841 aattgaaccc gagcccctcc tgaacggtcg tcggcgagcc ggtcttcgct ataagtatgt
     3901 cgccgggccg catctgccag cgcctcccgt cgcgctccac ccccacatag gtcaaccccg
     3961 ttttcgaata gtcgcggtag tcgaagaaca gccgcgtgtt gagccgcgtc gacagataag
     4021 gccccaccca cttctcgatg cggtatatga ccgaggtgtc gaacttcgcc atgtcgcggg
     4081 tgttgcgcac cttccggttg tcctgcatca tgagatcctc gaacaggtcg agccgggcgc
     4141 tgtagagcca gtcgtcgtca tcatacagcg ccgccgcgtt aagggcgcta ccgatggcga
     4201 gcgaattctc ggcgatagct ccttcgatca tgtagcccga gttggtgaac gacagcgacc
     4261 ccgacagcag gagacgcagg tcccagttcc ccttctgggt catctgctga tcgccagcca
     4321 cttcgccgcc gccgagcagt tgatgggtct tctcgtcgaa cacgagcaga acagtgctcg
     4381 cgtgccgttc gatcaggcgc accgtcacga agttgcgata cgaatcgggc gcctccccct
     4441 tcttcgagac acggtacagc cccggtcgca gtatccagat gagtggcttc tcgccgagtg
     4501 tttcgtcagt gccggaaccg gcgccgtagt acagtttggt ctcctcgtcg cttatctggt
     4561 acccctcgcg gagcgggttg cggtacgcgt ccacgacctg cacgacgagc gcgccccaca
     4621 ccggcatgac caccgtcacc cgttccggct tcaccgtgac atagaagacg atgcgggtat
     4681 cgagcggccc cgacccgatg tagagcacat agtcgcccgg gtcgagcacg acccgccgtc
     4741 ccgtctcggc gtcggtgatg aactcctcgc cgcggtagac cgcgtaggtc ggctcgcgct
     4801 ggctatcgac gagccacggg acgaacaccg cccccttctc catcgggacg agcgtcgcgt
     4861 cgcgcgtcaa ctgctcctgt atctgtgggt ccttgacctg ccgggcgtcg agggtgagcg
     4921 cccccagcgg aagggcgcac agcagtacga gcacaaaaaa agatgcgggg acgcggctac
     4981 tttttaccaa gttcggcggc gatatgttga atgaatctct ctatctcctg ttccgctatc
     5041 tcgccgatcg cccgaacggt atgcaccggt tcgcgaccga acacctcgcg ccgttcgtcg
     5101 aaggggtggg cggcgagcac cgcgccggtc ttgaaatcct tgagggtgag caccatgctc
     5161 aggtgggcga accagcggtc gccgctgtcg aacatatcta tctcgcgtat ctcgccggtc
     5221 acctcgtagt cgggggtctt gtcgagtttc acgatgagat cggtgaacag gccggccacc
     5281 ttgaggtgcg cgtagaccag atcggtcatc atgtcctcgg gacgggaggc ccagtagtgg
     5341 ttggggtagt agaatatctc gtagggtgag gtgcggtaca cgagattgta gcggcggtac
     5401 accttgtcta tctcgagcgt cttcacgcgg gcggtcgccg gcaccgggac cacgcgggcc
     5461 gtctgggggc gatagtaggc gcggtagtag tgctttttcg cctgagaccc gaaacagccc
     5521 ccgaaaagaa gcgccgcgca gaagacaaga cccagtgtcg ccgccgtccg tctcatttcg
     5581 tatcctccga tccggtgccc tgcaggatcg ccgccggatt ctccatgatg atgcgggtga
     5641 actcgttgaa gttcatcatg ctctccgaaa gattgtccag cgacttgttg atgtcctcgc
     5701 ggctccgcac gag
//
LOCUS       DAOQUE010000137         5704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15544_length_5704_cov_4.265180, whole genome shotgun sequence.
ACCESSION   DAOQUE010000137 DAOQUE010000000
VERSION     DAOQUE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15544_length_5704_cov_4.265180"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2120
                     /locus_tag="PKH10_05550"
     CDS             <1..2120
                     /locus_tag="PKH10_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27633.1"
                     /translation="GLLKDAKSGEPVSGTIMLKEQKLQVPVDAAGRFVLSLQPGVYTV
                     ELAIVGFVPEEKNIEVTGNSDVIWELIPVERDRHFYGYHEKISDKTVAGISDLPGVTI
                     AAPRRRYDTGFGIAGSPSPENGYYIEGLPSPFAFHLLGRDTVIHPALVGTAELSYGGF
                     SPEYGDATGGMVSLTLRDPREDRLGGMFDLSLFGVSVLAEGPVTEHDLFAAAVSKGLT
                     DLFPRFFYGEGSDLLYNDNFDFTMLYLRKFSGGHRLRVYAFGSMDSLGFRSGDLMNGV
                     PEHVDLIAPSQFFTRLVADHAYSWKELDVEIDNRVLLSYENRHWQYGFAAGDDFGLSS
                     HVIEGQDVFHWRFDPQHKLSLGGAMRLGFFSPATDYVSLPLEGEPFAVRVARKIDDPG
                     YQPYLHPSLWASYLFSYAGFTVEPGLLFYADFHNKKHYELSADPRLTTAYRFDDTMEL
                     RLSGGLYSRRPDYEIASDRWGTEDLLAEHAVHAVLSFEKTFLGLYTVTAKGFYKYLYN
                     LIRRSNADPLEFNNLGEGYAAGGVLALKIDIADTFRGTVGYAYTASQRKESPAGEYRP
                     SDAEVPHSVTLSGRWTPAKEWALSGRFSLFSGAPYSRFSGAEFIDDGIVARFEPLPIL
                     DGNGRVIRNDARYPVLHELDLRGEYTFFLDHTILVLFAEMRGISGLFDQNAVGSLYNT
                     DFTKNTPLSSTPFSATVGIRGEF"
     gene            2131..3012
                     /locus_tag="PKH10_05555"
     CDS             2131..3012
                     /locus_tag="PKH10_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27634.1"
                     /translation="MRTYLPTLGLLVLLVAGLGCENNALDPAWKITGPRIIALQADKQ
                     VFSPGDTVTFSVLLAGVSATERASISVLWRLGNEVRELAADRVARYEIPDENAAADVD
                     IFGTAAWSAYRTAGHVVVELQATVRMNDGTTLPARKSLLLAKRSVALQYEAPLIDRIL
                     VAVPGHSGLSLPAGETVRLPAGSPDTIAIEATLADNTTPYGYRWYIENTLSDGDAEIL
                     SGGVDRTMKMKMPQLGATIVYLVVEDRSEGSLSKVFNGGIDAVSFFISYGTDDVDDAD
                     TLSSDETDTDQDQLLPD"
     gene            3292..4164
                     /gene="gspC"
                     /locus_tag="PKH10_05560"
     CDS             3292..4164
                     /gene="gspC"
                     /locus_tag="PKH10_05560"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HNT27635.1"
                     /translation="MRAEASSRRAGNKRTPEVVPQEEMIYFGSPKGTASLPNAFDLSM
                     RQIALASAESNEAFSQPHEVTIEEINALLDTPERCIPLPGFALLGTALAIPESESLIT
                     VASSKSSAGEKGGTPLIMVLRIDDSTDDGALLAAVRRNLVAFKTSAGIRCLGENVGKS
                     ASLVEAEKPSNKVGDAAGDQFNVQQTGPNSYRISRDDLNRATANLNNLASEARIVPDK
                     KESGFKIFSIRPGSIWQKIGVQNGDVVKSINGIDLSSPDKALEAYSRLRNSNKLSLDV
                     VRRGKKETLEYTVD"
     gene            4177..>5704
                     /locus_tag="PKH10_05565"
     CDS             4177..>5704
                     /locus_tag="PKH10_05565"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNT27636.1"
                     /translation="MRRGMLLILLVAWLSMAVAQQPNEMQVPIKLNPIQQQPLKNALK
                     GSIKKEKVKKKNDRKKEEKVTDDPATGTPPPVEGEGSSSMQATATGRSAEAGETIKVE
                     SKHITPMSKNTKLYLDFPDTDLLAVIKFFSEKTGRNFIVQERLAKAKITIMSPVPVTI
                     DEAWRTFLTLLSVNNISVVQDGKFLIIMNEKDTVTQGIAVVKEDKIPDVFKMVATILK
                     FRYVPAVEMEKVIKIFRDPSGATLIFDEKTIIMVDYAANIRKIRDLTRDLDMPAEADT
                     ARLHFIHLNHIAPSDAKKLLDEIFKDFVAKGGKQQKKDTTTPVLGGVATDEKGQPPSP
                     AEGQQSAPSSGDDAANAISSMYVQIVADDRNEQIIILANKATFELVLQVVKKIDREIE
                     GEGEIHVLKLQNAKAKDLVTTLNTLTKGAGKTQAKGQAKGTEVFEGEVNISANEATNS
                     LVVVSSLRDFRNLRKVVEQLDIRRKQVFVEAVIMEVKVSEGLEYGTSFAAGGFETTIG
                     G"
BASE COUNT         1290 a         1543 c         1626 g         1245 t
ORIGIN      
        1 ccggtctcct gaaggacgcg aagagtggag aacccgtcag tgggacgatc atgctgaaag
       61 agcagaaact gcaagtgccg gtcgacgccg ccggtcggtt cgtgctctct ctgcaaccgg
      121 gcgtctacac cgtcgaactg gctatcgtcg ggttcgtgcc cgaggaaaag aacatagagg
      181 tgaccggcaa cagcgacgtg atatgggaac ttataccggt cgagcgggat cgccatttct
      241 atgggtacca cgagaaaata agcgataaga ccgtggccgg catctccgat ctccccggtg
      301 tgacgatcgc ggcgccccgg cgtaggtatg acaccgggtt cggcatcgcc ggctctcctt
      361 cgcccgagaa cggatactac atcgaaggcc tgccgtcacc gttcgccttt cacctgctcg
      421 gtcgtgacac ggtgatacac cccgctcttg tcggtacggc ggaattgtcc tacggagggt
      481 tctcgcccga atatggtgat gcgaccggtg gcatggtatc actgacgctt cgcgacccgc
      541 gggaggatcg gcttggcggc atgttcgatc tcagcctgtt cggcgtttcg gttcttgccg
      601 aaggtccggt gaccgagcat gacctcttcg cggcggcggt cagcaaggga ttgaccgacc
      661 tgttcccgcg gtttttctac ggtgaaggga gcgaccttct atataacgac aacttcgatt
      721 tcacgatgct ctatctgcgc aaattctccg gtggtcaccg gttgcgggtc tatgccttcg
      781 gtagcatgga cagtctcggg ttccgatcgg gcgatcttat gaacggcgtg ccggagcatg
      841 tcgatctcat cgcgccatcg cagttcttca cccgtctcgt ggcagatcat gcctattcgt
      901 ggaaggagct cgatgtcgag atagacaacc gggtccttct gtcctacgag aaccgtcatt
      961 ggcagtatgg cttcgcggcc ggtgacgatt tcggcctctc ttctcatgtg atcgaaggtc
     1021 aggatgtctt tcactggcgg ttcgaccccc aacacaaact ttctctcggt ggcgcgatgc
     1081 ggctgggctt cttctctccc gcgaccgatt atgtttccct gccgttggaa ggagaaccgt
     1141 tcgccgtacg ggtcgcccga aagatagatg acccgggcta tcagccatac ctgcacccgt
     1201 cgttgtgggc ctcgtacctt ttctcgtacg cggggttcac cgtggaaccg ggactcctgt
     1261 tctacgctga tttccacaac aagaagcact acgaactatc agcggatccc cggctcacga
     1321 ccgcctatcg gttcgacgac accatggagc tccgcctctc gggtggtctc tattctcgcc
     1381 gtcccgacta cgagatcgca agtgatcggt ggggcaccga ggacctgctc gccgaacatg
     1441 cggtccacgc cgtactttct ttcgaaaaaa cctttctcgg cttgtacacc gtaacggcga
     1501 agggttttta caagtatctt tacaacctga tccgccgctc gaacgccgat ccgcttgaat
     1561 tcaacaatct gggagagggt tacgccgccg gcggagtgct cgcactcaag atcgatatcg
     1621 ccgacacctt ccggggaacg gtcggctatg cctataccgc cagccaacgc aaggaaagtc
     1681 ccgccgggga gtatcgtccg agcgatgcag aggtccctca ttccgtgacc ctttcgggac
     1741 gctggacgcc agcgaaggaa tgggcgctgt cgggtcggtt ctccctgttc tctggcgcgc
     1801 catattcccg tttttccggg gcggaattca tcgacgacgg tatcgtcgcc cgtttcgagc
     1861 cgctaccgat cctcgacggg aacggtcgtg tcatacgcaa cgatgcgcgg tatccggtcc
     1921 tccatgaact tgacctgcgc ggcgagtata ccttctttct cgaccacacg atactggttc
     1981 tattcgccga gatgcgtggg ataagcggtc tgttcgacca gaatgcggtc ggcagtctct
     2041 acaataccga ttttacgaag aacacccctc tttcctccac gccgttctcg gcgacggtcg
     2101 ggatacgagg ggaattttag ggggcgcgtc atgagaacct atctgccgac acttgggttg
     2161 ttggtgctcc tcgttgcggg gctcggttgc gagaacaacg ctcttgaccc cgcatggaag
     2221 ataacggggc cgcggatcat cgccctgcag gccgacaaac aggtcttctc tccgggcgac
     2281 accgttacct tctcggtgct tctcgccggt gtcagcgcga ccgaacgggc tagcatatct
     2341 gtgctctggc ggctcgggaa cgaggtcagg gaacttgcgg ccgaccgcgt cgcccgctat
     2401 gagataccgg acgagaatgc cgccgccgat gtcgacattt tcggaacggc cgcatggtcc
     2461 gcgtaccgga ccgccggaca tgtggtggtc gaactgcagg ctaccgtgcg catgaatgat
     2521 ggcacgacat tgccggcccg caaaagtctg ctgttggcaa aaagatcggt agcgcttcag
     2581 tatgaggctc ccctgataga ccgtatcttg gtggcggtcc cgggccacag tggattgtcg
     2641 cttcccgcag gggagaccgt tcgtctgcct gctggatcac ccgacaccat cgcgatcgag
     2701 gctacccttg ccgacaacac gacgccctac gggtatcgtt ggtatattga gaacaccctc
     2761 tcggacgggg atgccgagat attgagtggc ggggtcgatc gcaccatgaa aatgaagatg
     2821 cctcaattgg gggcgaccat cgtgtatctg gtggtggaag atcgatccga gggatcgctg
     2881 tccaaagtgt tcaatggcgg tatcgatgcc gtttcctttt tcatttcgta cgggacagat
     2941 gatgtcgatg atgccgatac actgtcttcc gacgagaccg atactgatca agaccaactt
     3001 cttcccgact gacctccttt gtgagctagg cgaggctctt tctgtcaaac tgcgattgac
     3061 attttgacaa aacaagagga ttctctcctt tatgtcaagc tattgttttg ttattattcg
     3121 ctttatttaa aggcacgatg attgcataaa agtggaccgt ttttttgttt ggagtttcgc
     3181 gtgagaaatc gagccggtct cgcagtggtt ttgttcgtgt cgaccgcttt tctcttcttc
     3241 tcggtgtacg ctctggcgat atcgttcgtc ctcaaggagg gggagggtga cgtgcgcgcc
     3301 gaggcttctt cccgcagagc gggaaataaa agaacgccgg aggttgtccc gcaggaagag
     3361 atgatctatt tcggttctcc taaaggaacc gcctcgcttc ccaacgcctt tgatctgagt
     3421 atgcgccaaa tagcactggc ctcggcggag agtaacgagg cattctccca accgcacgaa
     3481 gtgaccatcg aggagataaa tgctcttctc gacaccccgg agcgttgcat ccctcttccc
     3541 ggatttgcct tgctcggcac agccctcgct atccctgagt cggaatcgct catcacggtc
     3601 gcgtcttcaa agagcagcgc aggagaaaaa gggggaacac cccttatcat ggtgctgcgt
     3661 atcgacgata gcaccgatga tggtgccctg cttgcggcgg tccggcggaa ccttgtggct
     3721 ttcaagacat cagccggtat ccgttgcctt ggggaaaatg tgggaaagag cgcctcactt
     3781 gtcgaagccg aaaagccctc caataaagtg ggggatgctg ccggggatca attcaatgtg
     3841 cagcagacgg ggcccaactc gtatcgcatc agtcgggacg atcttaaccg cgccacggcg
     3901 aacctcaaca atctggcctc cgaggcgcgt atcgttccgg acaaaaagga aagcgggttt
     3961 aagatattct ccatccgccc cggaagcatc tggcaaaaga tcggggtcca gaacggtgat
     4021 gtggtcaaga gcataaacgg catcgatctt tcaagcccgg ataaggcgct tgaggcatac
     4081 tcccggctca ggaattccaa caaactaagt ttggatgtcg tcaggcgcgg taagaaggaa
     4141 acactggaat atactgtgga ctaaatagga gttgtcatgc gtagaggaat gctgttgata
     4201 ctgttggtcg cgtggttgag tatggcagtt gcccaacaac cgaacgagat gcaggtgccg
     4261 ataaaactca acccgatcca gcaacaaccg ttgaagaatg cgctgaaagg gagtatcaag
     4321 aaggaaaagg tcaaaaagaa aaacgaccgc aagaaagaag aaaaggtaac ggatgatcct
     4381 gccaccggta ccccgcctcc ggtcgaagga gagggctcct cttcgatgca ggccacggca
     4441 accggtagga gcgctgaagc gggtgagacc atcaaagtag aaagcaagca catcacaccg
     4501 atgagtaaga acaccaaact ctatcttgat tttccggata ccgacctgct cgccgtcatt
     4561 aaattcttca gcgaaaaaac gggacgcaac ttcatcgtgc aggaacggct tgctaaagca
     4621 aagatcacca tcatgtcgcc ggtcccggtg acgatagacg aggcgtggcg cactttcctc
     4681 actcttttgt cggtcaacaa catttcggtt gtgcaggacg ggaagttcct catcatcatg
     4741 aatgagaagg ataccgttac gcagggcatc gccgtggtca aggaggataa gatacctgat
     4801 gtgttcaaaa tggtcgcgac catcctcaaa tttaggtatg ttccggccgt ggagatggaa
     4861 aaggtcataa agatattccg tgacccgtcg ggcgcaacac ttattttcga cgagaagacc
     4921 attattatgg tcgattatgc tgctaacatc cgaaagatac gagatctaac ccgcgatctt
     4981 gatatgccgg ccgaggccga taccgcgcga cttcatttta tccacctgaa ccatatagcc
     5041 cccagcgatg ccaaaaaact gctggacgaa atattcaagg atttcgttgc caagggaggc
     5101 aaacaacaaa agaaggacac cacgacaccg gtattgggtg gtgtggcgac cgacgaaaaa
     5161 ggacagccgc cttctcccgc agaggggcaa cagtcggcgc cgtcgagtgg agatgatgcc
     5221 gcgaacgcca tttcctcgat gtatgtgcag atagtcgccg atgaccgcaa cgaacagata
     5281 ataatccttg cgaacaaagc gaccttcgaa ctggtcctgc aggtggtcaa gaagattgat
     5341 cgcgaaatag agggggaagg ggagatacat gttctgaaat tacagaacgc caaggcgaaa
     5401 gatctggtca ccacactgaa cactctgacg aaaggtgccg ggaaaacgca agcaaaggga
     5461 caggcgaaag ggaccgaggt cttcgaggga gaggtcaata tctcggccaa cgaggcgacc
     5521 aattcgctcg ttgtggtgag ttctctgaga gattttcgta accttagaaa agtggtcgaa
     5581 caacttgaca tccgccggaa acaggtattt gtcgaggcgg tcatcatgga ggtgaaggtt
     5641 tccgaaggac ttgaatacgg cacctcgttc gccgcgggtg gttttgagac gaccatcgga
     5701 gggg
//
LOCUS       DAOQUE010000138         5699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15566_length_5699_cov_4.586109, whole genome shotgun sequence.
ACCESSION   DAOQUE010000138 DAOQUE010000000
VERSION     DAOQUE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15566_length_5699_cov_4.586109"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PKH10_05570"
     CDS             <1..292
                     /locus_tag="PKH10_05570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT27637.1"
                     /translation="AGDMEGFGLAALEAAICGLPVVASEIDGIVDAVQEGKNGFLLPP
                     EEPELWAHRITGLLADPAGLRAAGEAAREFTKEHYSWERMVDGYEAVFKRFA"
     gene            294..1430
                     /locus_tag="PKH10_05575"
     CDS             294..1430
                     /locus_tag="PKH10_05575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT27638.1"
                     /translation="MRTLFDCAVVGGGPAGAAAALTLARRGRRVILLEKEPLPRSKPC
                     GGGMPPSVLSLLALPSIDGIVDRVHRLTFLYDGGSPVSHELAEPLLMVDRARFDHFLV
                     RTAGAAGAEVRDRTAVRSFGATGDHWEIGTSAGTFAARKIILTDGGQGRCAALAGFPP
                     NRRTEGLALACELPADRRRDELTVDLGLLPCGYLWIFPKGDRISLGVGITKRGGNHLQ
                     LFDKMAVFAGRQGLPFDRSLCRGHRVAIWDGARPLTRLDSFLLAGEAAFLADPLSAEG
                     IRPAVKSGIAAGEAVDASLGGDATAFSRYEERMRRELGREFAIAARIAKFFFLFPSLS
                     YRAAFFHPRTTAIMNEVFLGRVAYSDLAGKVLSTLARTVKRRPL"
     gene            1554..2504
                     /locus_tag="PKH10_05580"
     CDS             1554..2504
                     /locus_tag="PKH10_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT27639.1"
                     /translation="MKKILIIGAVGQIGSELTLRMRELYGNQNVIASTRKTPPSDKIK
                     ESGPFEFFDVMDRKKLEEVVDKYKVEYIVNMAAILSAVGEKDPMFAWDVNMNGLINIL
                     EVAREKKIKQILMPSSIAVFGPGTPRVNTPQDTVLKPTTMYGVTKVAGELLCDYYVRK
                     YGMDIRGLRYPGIISHETLPGGGTTDYAVAIYYEAIQHGKYTCFVKESTKLPMMYMPD
                     CLKATIDLFEADFSKLKHHSDFNVAGMSFTVGEMATEIRKHIPDFKIDYKPDFRQAIA
                     DSWPESIDDTAAREEWGWKPAYTLASMTVDMLTELKKKLK"
     gene            2517..3740
                     /locus_tag="PKH10_05585"
     CDS             2517..3740
                     /locus_tag="PKH10_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT27640.1"
                     /translation="MSLTKLNKALTAHVNGLKEKGTAKGAEMVITKVLPPKGANGPRY
                     IVEGYKQEFIKMNSNSYLGLSMHPAVIHAEEEGTKIYGVGPGAVRFISGTHKPHIELE
                     NRLAKFHGKESGMIFSSAYVTSLGVITPLVTETTVIVSDELNHNCIINAMKLARPAAK
                     MIYKHNDMADLEAKMNEAVGKGTRCVVVTDGVFSMRGDYCPFDQFVKICEKYNDKFED
                     GVTTVADDSHGVGAYGATGRGTEEVTGTKVDIIIGTLGKAFGVNGGYVVASKAVTDFL
                     RQTAPMYIYSNPITLGETVAVLKVLDILESEEGKKRIKHLAEMTARFEKGLVDNGYET
                     IPGPHPVTPLMVRDTQKTADIVKWLTDHGVLATGLNYPVVPKGSEEIRFQINADHTPA
                     DVDYVIDVLKKYKNR"
     gene            complement(3759..4847)
                     /locus_tag="PKH10_05590"
     CDS             complement(3759..4847)
                     /locus_tag="PKH10_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27641.1"
                     /translation="MTRRMLLFLGLFAVLTVACDNETKKGAVDEDLVADDDELAVTDD
                     DTVASDTTEETPDLAQDEIVFPDADGGFDPENSGYNIFFQIMTMDVFGQTSGMAFGLF
                     LKTAREDLVVEEEDLLAIDTCVFEAGGATPTPSCTSKEDCAPEQDCLPETDNNGAPIA
                     GSERCVTPDRESLDRGPVTITGFVGGPATFAYEPNDKIYKKDGTGDGSVPVDILAFDA
                     TYELSGEGMEDLAAFSGSVYFPPALKLTAPEPHTGTAFPIPAITVDPTKDLTVTWDGA
                     DPDGTIDISMTGESGSVVCRATDDGSFTMPADLIGQITFGTGFGAMANNLILDRKYSE
                     KMEGKSVTAGTFDTEQMMMIMVEPVTGW"
     gene            complement(4908..5495)
                     /locus_tag="PKH10_05595"
     CDS             complement(4908..5495)
                     /locus_tag="PKH10_05595"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNT27642.1"
                     /translation="MPKMILASASPRRREMLTLFSFPLEIDAPVVDETPIPGLAPAAL
                     AEMLAERKGSSVVGRRGDGDPVIAADTVVVSGGELLGKPRDTEDAERMLRLLSGTDHE
                     VVTGVALWWRGKKQVFSVTSTVTFKSLKEKEISDYIATGEPMDKAGAYAIQGIGGFMV
                     RSVTGSVSNVVGLPIAEVIAALQEMGFLRGLTIYT"
     gene            complement(5509..>5699)
                     /locus_tag="PKH10_05600"
     CDS             complement(5509..>5699)
                     /locus_tag="PKH10_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27643.1"
                     /translation="ASLLQDGGWPPVFIADEIFSFMDAERRAKAWSLLDELPIQTIST
                     FHREKEHRWGSAVQMIKL"
BASE COUNT         1130 a         1853 c         1626 g         1090 t
ORIGIN      
        1 cgcgggcgac atggagggtt tcggcctcgc ggcactcgag gcggccatct gcggtctgcc
       61 ggtcgtcgcc tcggagatcg acggcatcgt ggacgccgtg caggagggaa agaacggctt
      121 cctcctgccg cccgaggagc cggaactctg ggcccaccgc atcaccggac tgctcgccga
      181 cccggcgggg ctccgcgcgg cgggagaggc ggcgcgagag ttcacgaagg aacactattc
      241 gtgggaacgg atggtggacg gttacgaggc ggtcttcaag cggttcgcct gacatgcgga
      301 cgctgttcga ttgcgcggtg gtcggcggcg gtcccgccgg agcggccgcg gccctgacac
      361 tcgcccggcg cgggcgacgc gtcatcctat tagaaaagga accgttgccc cgttccaaac
      421 cgtgcggtgg cggcatgcct ccttcggtgc tctcgctcct cgctctccct tccatcgacg
      481 gcatcgtcga ccgggtccac cggctcacct tcctctatga cggcggttct cccgtttcac
      541 atgaacttgc cgagccgctc cttatggtgg accgtgcccg tttcgaccat tttcttgtga
      601 gaacggccgg cgcggccggc gccgaggtcc gcgaccggac cgccgtgcgt tccttcggcg
      661 ccactggcga ccactgggag atcggcacga gcgccggcac ctttgcggcg cggaagatca
      721 tcctcaccga cggcggacag ggaagatgcg ccgcactcgc cgggttcccg ccaaacagaa
      781 ggaccgaagg gctcgcgctc gcctgcgaac tgccggccga ccggcggcgc gacgaactga
      841 cggtcgatct cgggctccta ccgtgcgggt atctatggat cttcccgaaa ggcgaccgga
      901 tttcgctcgg tgtcggcatc accaaaagag gcggcaacca cttgcaactg ttcgataaaa
      961 tggctgtctt cgccgggcga cagggcctgc cgttcgatcg gtcgctctgt cgcggacacc
     1021 gtgtcgcgat atgggacggc gcccggccgc tcacccgtct cgattcgttc ctgcttgccg
     1081 gcgaggcggc gttcctcgcc gacccgctga gcgccgaggg gatacgcccc gcggtgaaaa
     1141 gcggcatcgc ggcgggcgag gcggtggacg cgtcgctcgg cggcgacgcg accgcctttt
     1201 cacgctacga ggaacggatg cggcgcgagt tggggcgcga attcgccatc gccgcgcgga
     1261 tcgcgaagtt cttcttcctg ttcccctccc tgtcgtaccg cgccgccttc tttcacccgc
     1321 gcaccacggc gatcatgaac gaggtgttcc tcggacgcgt cgcctactcg gacctcgccg
     1381 ggaaggtgct ttcgacgctc gcgcggacgg tgaagcgaag gcctctctaa aaaggaaccc
     1441 ctctctcttc tctcttcgtg ttttccttga caccgaacgg tgcaacggta tattcgcact
     1501 gtttttaaaa cggtcgttcg gcgattataa acctgttctt cgtggagaga cccatgaaaa
     1561 agatcctcat catcggtgcc gtcgggcaga tcggctccga actcacgctc aggatgcgcg
     1621 aactgtacgg caaccagaat gtcatcgctt ccaccagaaa gacccctccc tctgacaaga
     1681 tcaaggagag cggtccgttc gaattcttcg atgtcatgga ccgcaagaag ctcgaagaag
     1741 tggtcgacaa gtacaaggtc gaatacatcg tcaacatggc cgccatcctc tccgccgtcg
     1801 gcgagaagga cccgatgttc gcgtgggatg tgaacatgaa cggcctcatc aatatccttg
     1861 aagtggcccg cgagaagaag ataaagcaga tactcatgcc gagctccatc gccgtcttcg
     1921 gccccggcac gccccgcgtc aacaccccgc aggacaccgt tctcaagccg accaccatgt
     1981 acggcgtcac caaggtcgcc ggcgaactgc tgtgtgacta ctatgtccgc aaatacggca
     2041 tggacatccg cggcctccgt tatccgggca tcatcagcca cgagacgctg cccggcggcg
     2101 gcaccaccga ctacgcggtc gcgatatact acgaggcgat ccagcacggc aagtacacct
     2161 gcttcgtcaa ggagtcgacg aaactcccga tgatgtacat gcccgactgt ctcaaggcga
     2221 ccatcgacct gttcgaggcc gatttcagca aactgaaaca ccactccgac ttcaatgtcg
     2281 ccggcatgtc cttcaccgtc ggcgagatgg cgaccgaaat ccgcaagcat atacctgatt
     2341 tcaagataga ctacaagccc gacttccgtc aggcgatcgc cgattcgtgg ccggagtcga
     2401 tcgacgacac cgcggcgcgc gaagaatggg gctggaagcc ggcctacacg ctcgcctcga
     2461 tgacggtcga catgctgacc gaactcaaga agaaacttaa ataaaaggag cgaaccatgt
     2521 cactgacgaa actgaataag gcgctcaccg cccatgtgaa cggcctcaag gaaaaaggca
     2581 ccgcgaaagg cgccgaaatg gtcatcacga aggtccttcc gccgaaaggc gcgaacggtc
     2641 cccgctatat cgtcgaaggc tacaaacagg aattcatcaa gatgaactcc aactcctacc
     2701 tcggcctgtc gatgcacccg gcggtcatcc atgccgaaga ggaagggacc aagatctacg
     2761 gcgtcggccc cggcgcggtc cgcttcattt ccggtacgca taagccgcac atcgaactcg
     2821 agaaccggct cgcgaagttc cacggcaaag agtcgggcat gatattctca agcgcgtatg
     2881 tcacctcgct cggtgtcatc acccctctcg tgaccgagac gaccgttatc gtgtcggacg
     2941 aactgaacca taactgcatc atcaacgcga tgaaactggc ccgacccgcc gcaaagatga
     3001 tatacaagca caacgacatg gccgatctcg aggcgaagat gaacgaggcg gtcggcaaag
     3061 ggacccgctg cgtggtcgtt accgacggcg tcttctcgat gcgcggcgat tactgcccct
     3121 tcgaccagtt cgtcaagatc tgcgagaagt ataacgacaa gttcgaggac ggtgtcacca
     3181 ccgtcgccga cgattcgcac ggtgtgggcg cgtacggcgc gaccgggcgc ggcaccgaag
     3241 aggtcaccgg cacaaaggtc gacattatca tcggcacgct cggcaaagcg ttcggcgtca
     3301 acggcggata tgtggtcgca agcaaggcgg tcaccgattt cctgcgccag accgctccga
     3361 tgtacatcta ctccaacccg ataacgctcg gcgagacggt cgcggtcctc aaggtcctcg
     3421 atatcctcga gagcgaagag ggcaagaagc gtatcaagca tctcgccgag atgacggccc
     3481 gtttcgagaa aggcctcgtg gacaacggct acgagaccat ccccggcccg catccggtca
     3541 ccccgctcat ggtgcgcgac acccagaaga cggccgacat cgtgaagtgg ctcactgacc
     3601 acggtgtgct cgccaccggc ctcaactatc ccgtcgttcc gaagggttcg gaggagatcc
     3661 gcttccagat caacgccgac cataccccgg ccgatgtcga ttatgtcatc gatgtgctga
     3721 agaaatacaa gaaccgttga taccgttccc ggtagccctt accacccggt gaccggttcc
     3781 accatgatca tcatcatctg ctcggtatcg aaggtcccgg cggtcacgct ctttccttcc
     3841 attttttccg agtatttacg gtcaagtatc agattgttag ccatcgcgcc gaagccggtc
     3901 ccgaaggtta tctgaccgat gaggtcggcc ggcatcgtga aggaaccgtc atcggtggcg
     3961 cggcaaacga ccgaaccgct ctcgccggtc atcgatatgt ctatcgtccc gtcggggtcg
     4021 gcaccgtccc aggtcacggt gagatccttg gtggggtcga ccgttatcgc cggtatcggg
     4081 aatgcggtcc cggtgtgcgg ctcgggcgcc gtgagtttca gggcgggcgg gaaatagacc
     4141 gatccgctga acgccgcgag atcctccatc ccctcaccgc tcaattcgta ggtcgcgtcg
     4201 aacgcgagga tatcgaccgg gaccgacccg tcgcccgtgc cgtctttctt gtagatcttg
     4261 tcgttcggct cgtaggcgaa ggtcgccggc ccgccgacga acccggtgat ggtgaccggt
     4321 cccctatcga gcgattcgcg gtccggcgtc acgcaccgtt cgctccccgc tatcggggct
     4381 ccgttgttgt cggtctccgg caggcagtcc tgctccggtg cacagtcttc tttcgaggta
     4441 cacgacggtg tcggcgtcgc gccgcccgct tcgaacacgc aggtgtcgat cgccagcagg
     4501 tcctcctcct cgaccacgag atcctcgcgc gccgttttga ggaacaggcc gaacgccatg
     4561 cccgaggtct ggccgaacac atccatcgtc atgatctgga aaaagatgtt atagccggaa
     4621 ttctcggggt cgaaaccgcc gtcggcgtca ggaaacacta tctcgtcctg cgcgagatcg
     4681 ggcgtctctt cggtcgtgtc ggaagcgact gtgtcatcat cggtcaccgc caattcatcg
     4741 tcatcggcga caagatcctc gtcgaccgcg ccctttttcg tttcgttgtc gcacgcgacc
     4801 gtgagcaccg cgaaaagtcc caaaaaaagc aacatccttc tcgtcatggt ctcctcctct
     4861 ttacgaatgc attggaccct cggatatttc atgtaccgcc ttttttgtca agtatatatc
     4921 gtgagccccc ggagaaaacc catctcctgc agggccgcta tcacctcggc gatcggcagg
     4981 ccgaccacat tcgagaccga gcccgtgacc gatctcacca tgaagccccc tatcccttgt
     5041 atcgcgtacg cccccgcctt gtccatcggt tctccggtcg cgatatagtc tgatatctcc
     5101 ttttctttca atgacttgaa tgttaccgtg ctcgtcaccg agaacacctg tttcttacca
     5161 cgccaccaga gcgcgacacc ggtcacgacc tcatggtcgg tgccggacaa taaacggagc
     5221 attcgctctg catcctcggt atctctgggc tttcccagca attcaccgcc agacaccacc
     5281 accgtgtcgg ccgcgatgac cgggtcaccg tcaccacgcc gaccgacgac cgacgacccc
     5341 tttctttctg ccagcatctc ggcaagcgcg gcgggcgcaa gccccggtat cggggtctcg
     5401 tccaccactg gcgcatcgat ctcgagtggg aacgaaaaaa gggtcagcat ctccctgcgg
     5461 cgggggctgg cgctggcgag aatcatcttc ggcatccgtc atctctcctc acaatttgat
     5521 catttgcacg gcgctccccc accgatgttc tttctcccgg tgaaaggtgc ttatcgtctg
     5581 tatcggaagt tcatcaagta acgaccatgc cttcgcgcgc cgttcagcgt ccatgaacga
     5641 gaatatctcg tcggcgatga acaccggcgg ccacccgcca tcctgcagga gcgatgcct
//
LOCUS       DAOQUE010000139         5681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15631_length_5681_cov_5.132599, whole genome shotgun sequence.
ACCESSION   DAOQUE010000139 DAOQUE010000000
VERSION     DAOQUE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15631_length_5681_cov_5.132599"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1200)
                     /locus_tag="PKH10_05605"
     CDS             complement(22..1200)
                     /locus_tag="PKH10_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27644.1"
                     /translation="MRRCLPIIALLFLVAAACAPQGSPATAEGVRYFSVSFAPGTDTG
                     TPSAPLPETTEYAFAVTVEARDLWGDRVTGFSGEVEVSLLGATLRSYRRISLMNGTSD
                     PVTVRFGDGYRVERIAVTAIATEKTIVDAREIRLPTGPVGVSDPIYLISPRIPAVQGS
                     LASVDDGGPSRLEKRNLTIRGPYDTAAGRHRDLVVIAAIEGGFYLSETDCEEFCSIYL
                     YTHSTPYVEDGDDRAVVAPGMLLAEVNGSVFEYFGFTELSFPTFTPRRDGDGRILRDP
                     AAIPAPRDVTACVDAADDLCLEGHEAMLVTVTGIAVDDFDEQDEAWSLYSQFPLRTSA
                     GGVVMAQTVYTAPSFDPVAMKGVPIVSLTGLLKQHTSSRPSTWILVPRDGQDIVTGGM
                     "
     gene            complement(1200..3254)
                     /locus_tag="PKH10_05610"
     CDS             complement(1200..3254)
                     /locus_tag="PKH10_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNT27645.1"
                     /translation="MKSTCAISLLAALLAAGCLGESYGTAPEDPATPRELVLLHTTDS
                     HSKYFPFWIQPNMFDRAMGISSRNPHCWDLDWAGGAAWCDGAWIAEKGRFKFWEDGDY
                     IYLTEEEMTARKLVSEDVNRDGTCDIHDCGIVWDRDRDGTCSFPYDTATGSYRAADGT
                     VIDPADRIAFTAARNASEDVDRNGRCDDLDYRPELASTGGVARIATILRRIREERAAF
                     PVIHIDTGDAFQGAPQFNLFRGEVEMRALAELGIDAMVIGNHEFDNGTRFLVEAYERS
                     GGFPLLASNYLFDDRVSTGLAGILQPFIILHRGSLRIGVVGIGNDSSMTSMSDIGNKL
                     GFYGLDPVETAKLYVGLIRAQCDIVVIASHQGLEGDYELAAQVPGVDIILGGHHHVVL
                     DPPKVLTGPDGREVLIVHSGVDLKIVGELRVIVKDGRVAWHAYTVHPNTDAVAEDPAM
                     THALQPYQESLRLTQDLTTVVGYAKNMIRRTDPNGGDSPLGNLVTDAMMRHELVRAEI
                     AVTNSLGIRADIPAGDIRREKLYEVFPFENTITTMYLSGRELKDMFDYIARRSASRGC
                     VTQVQVAGLSVELDCSADTDLQERYNSYALTKELRIGDATVIADYELKLPHTLFKMAT
                     NDYMARGGSGFDMLLRNTTRIDTSIPIRDALVDLMREQGEIDPADFSSEKTGVRRITM
                     RN"
     gene            complement(3269..4876)
                     /locus_tag="PKH10_05615"
     CDS             complement(3269..4876)
                     /locus_tag="PKH10_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27646.1"
                     /translation="MLVLAYRWLLITGLLWCAGCNLDPDKSWFTDREATGDGDDIAID
                     GDQPAALSDDGPPLQDDAVSDTDTVVPDDAPVSLGVIGDIQQGTVAVDSPVSFEAVVT
                     AVEYVLDTEYAPTGIKGLFVSQAGLDTALPWSGIYVFIKTPAAVDAYARGDLLEISGT
                     YKEYYGASQVENATVGKLSSADIPAPALIADPSSIATPFALSNDEWLPTTAHGAAAEQ
                     WESVLIEVHDVTVTNEDLGHGQWEVTGGLVVDKKLFYYPGERSTGTRFKRITGILVYT
                     YDAFKIAPRDADDLELDDGTATDDDTVTTDDGPVTDSDQVVAGTIAQIQQGEVALDTT
                     VSFEAVVTAVEYALDTEYAPTGIQGLFVSQAGLDTALPWSGIYVFIKTPAAVDAYARG
                     DLLEISGTYKEYYGASQVENATVGKLSSADIPAPALIADPSSIATPFALSNDEWLPTT
                     AHGAAAEQWESVLIEVHDVTVTNEDLGHGQWEVTGGLVVDKKLFYYPGERSTGTRFKR
                     ITGILVYTYDAFKIAPRDADDVAPATR"
     gene            5059..>5681
                     /locus_tag="PKH10_05620"
     CDS             5059..>5681
                     /locus_tag="PKH10_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNT27647.1"
                     /translation="MKAFLVSLAFCSALLCLACVEDDCVYCGKDVSGGVTYDADAHKP
                     LVDKDTVKPDGQTVADQDTAEPDEELSDADTLVPDDNFVVADRMVLVPGDTFNMGCLD
                     VEDPACAETHSSPRHEVTLPDFEIDIYEVTKQEFEACIKAGGCKNDDGEPVQYVAYDA
                     DQNDYCVIYGVYDKDLPVNCVSWYGARAYCEQADEEERLGEHGEEDR"
BASE COUNT          967 a         1752 c         1914 g         1048 t
ORIGIN      
        1 tctttaggaa gagggggtcc atcacatgcc tcctgtcacg atatcctgcc cgtcgcgcgg
       61 cacgaggatc caggtcgagg gacgggaact cgtatgctgt ttgaggagcc cggtcagcga
      121 aacgatcggc acgcccttca tcgcgaccgg atcgaacgac ggggcggtgt agacggtctg
      181 cgccatgaca acgcctcccg ccgaggtccg gagcgggaac tggctgtaga gagaccaggc
      241 ctcgtcctgc tcgtcaaagt cgtccaccgc gatgccggtc accgtgacga gcatcgcctc
      301 gtggccttcg aggcagagat cgtcggccgc atcgacgcag gcggtgacat cacgtggcgc
      361 cggtatcgcc gccgggtcgc gcaggatgcg cccgtcgccg tcgcgtcgcg gcgtgaaggt
      421 ggggaaggag agttcagtga agccgaaata ctcgaacacc gagccgttca cctcggcgag
      481 gagcatgccg ggggccacca ccgcgcggtc gtcgccgtcc tcgacatagg gggtcgagtg
      541 ggtgtagagg tatatggagc agaactcctc gcagtcggtc tccgaaagat agaagccccc
      601 ctcgatcgcc gcgatgacca cgaggtcgcg atgtcgcccc gccgccgtgt cgtacgggcc
      661 gcggatcgtc agattgcgtt tctcgagtcg tgacgggccg ccgtcatcga cgctcgcgag
      721 actcccctgc accgccggta tgcgcggaga aatgagatat atggggtcgg atacgccgac
      781 cggaccggtc gggagccgta tctcgcgcgc atcgacaatg gtcttttcgg tcgctatcgc
      841 cgtgaccgcg atgcgttcga cgcggtatcc gtcgccgaac cggaccgtta ccgggtcgct
      901 cgtcccgttc atgagcgata ttcgccggta ggatcgaagc gtcgcgccga gcaggctcac
      961 ctcgacctcg cccgagaaac cggtcacgcg gtcgccccac aggtcgcgcg cttctaccgt
     1021 caccgcgaag gcgtattcgg tcgtttcagg gagcggggcg cttggcgttc cggtatcggt
     1081 gccgggcgcg aaagagaccg agaaatagcg tactccctcg gcggtggcgg ggcttccctg
     1141 aggcgcgcag gcggcggcga caaggaacag caacgcgatg atcggcaggc accttctcat
     1201 cagttcctca tggtgatgcg gcggaccccc gtcttttcgg acgagaaatc ggccgggtct
     1261 atctctccct gctcccgcat gagatcgacg agcgcgtcgc ggatcggtat cgaggtgtcg
     1321 atgcgcgtgg tgttgcgcag gagcatgtcg aaccccgagc cgccgcgcgc catgtagtcg
     1381 ttggtcgcca tcttgaacag cgtgtgaggc agtttgagct cgtagtccgc tatcaccgtt
     1441 gcgtcgccga tgcgcagttc cttggtcagc gcgtaggagt tgtagcgttc ctgcaggtcc
     1501 gtgtcggccg agcagtccag ctcgaccgag agcccggcga cctgcacctg cgtcacgcag
     1561 ccccgggatg ccgagcgacg ggcgatatag tcgaacatgt ccttgagttc ccggcccgag
     1621 agatacatgg tcgtgatggt gttctcgaag ggaaagacct catatagttt ctcgcgccgg
     1681 atgtcgccgg ccggtatgtc ggcgcggatg ccgagactgt tggtcaccgc gatctcggcg
     1741 cgcaccagtt cgtgccgcat catcgcgtcg gtcacgaggt tgccgagcgg cgagtcgccg
     1801 ccgttcggat cggtgcgccg tatcatgttc ttcgcgtagc cgaccaccgt cgtaagatcc
     1861 tgcgtgagtc tgagcgattc ctggtagggc tggagcgcgt gggtcatcgc cgggtcctcg
     1921 gcgaccgcgt cggtgttggg gtggacggtg taggcatgcc atgcgacgcg accgtccttc
     1981 acgatgaccc gcagttcccc gacgatcttg aggtcgacgc cgctgtggac gatgagcacc
     2041 tcgcggccgt cgggtcccgt gagcaccttc ggcgggtcga gcacgacatg gtggtggccg
     2101 ccgaggatga tatccacccc gggcacctgc gcggccagtt cgtagtcgcc ttcgagtcct
     2161 tgatggctcg caatgacgac gatatcgcac tgggcgcgga tgagaccgac atacagtttc
     2221 gccgtctcga ccggatcgag cccgtagaac ccgagtttgt tgccgatgtc gctcatcgag
     2281 gtcatcgagg agtcgttccc gatgccgacc accccgatgc gcagtgagcc gcgatgcagg
     2341 atgatgaacg gctggaggat cccggccagt ccggtcgata cgcggtcgtc gaacaggtag
     2401 ttcgaggcga gcagggggaa gccgcccgat cgctcgtagg cctcgacgag gaagcgggtg
     2461 ccgttgtcga attcatggtt gcctatcacc atcgcgtcga tgccgagttc ggcgagcgcg
     2521 cgcatctcga cctcgccgcg gaacaggttg aactggggcg ccccctgaaa ggcgtcgccg
     2581 gtgtcgatgt ggatgacggg gaaggcggcg cgttcctcgc ggatccggcg caggatcgtc
     2641 gctatgcggg cgaccccgcc ggtcgaggca agttcggggc ggtaatcgag atcgtcgcac
     2701 cggccgttgc ggtccacatc ctcgctggca ttcctcgccg cagtgaaggc gatacggtcg
     2761 gccggatcta tcaccgttcc gtcggcggcg cggtagctgc cggtcgcggt gtcgtagggg
     2821 aacgagcagg tcccgtcccg gtcgcggtcc cagacgatgc cgcagtcgtg gatatcgcag
     2881 gtcccgtcgc ggttcacatc ctccgacacg agtttacggg cggtcatctc ctcttcggtc
     2941 aggtagatat aatcgccatc ctcccagaac ttgaagcgtc ccttttcggc gatccacgcg
     3001 ccgtcgcacc acgcggcgcc gcccgcccag tcgaggtccc agcagtgcgg attgcgcgaa
     3061 gagatgccca tcgcgcggtc gaacatgttg ggttgtatcc agaaggggaa atacttggaa
     3121 tgcgagtcgg tggtatgcag gaggaccaat tcgcgcggcg tcgccggatc ttcgggcgcc
     3181 gtgccgtacg actcgccgag acagccggcg gcgagaagcg cggccagaag ggagatggcg
     3241 caggtgcttt tcatccccgc gaccctcctc accgggtggc cggcgcgaca tcgtccgcgt
     3301 cgcgcggggc gatcttgaat gcgtcgtagg tgtagacgag gatgccggtt atccgtttga
     3361 accgcgtgcc ggtcgagcgc tcgccgggat aatagaagag tttcttgtcc accacgagcc
     3421 cgccggtcac ctcccactgg ccgtggccga ggtcctcgtt ggtgaccgtg acatcgtgca
     3481 cctcgatgag cacgctctcc cactgctcgg ccgccgcgcc gtgggcggtc gtcgggagcc
     3541 actcgtcgtt gctcaacgcg aacggcgtcg cgatgctcga cgggtcggcg atgagcgcgg
     3601 gggccgggat gtcggcggaa ctgagtttgc cgacggtcgc gttctcgacc tgcgaggcgc
     3661 cgtagtactc cttgtaggtg ccgcttatct caagcaggtc gccacgggcg taggcgtcga
     3721 ccgcggcggg tgttttgatg aagacataga tgccgctcca cggcagggcg gtgtcgagcc
     3781 cggcctgact gacgaacagg ccctggatgc cggtcggcgc gtactcggta tcgagcgcgt
     3841 attcgaccgc cgtcaccacc gcttcgaacg aaacggtggt gtcgagcgcg acctctccct
     3901 gctggatctg cgcgatggtc cccgcgacga cctgatcgct gtcggtcacg gggccgtcat
     3961 cggtcgtgac cgtatcatcg tcggtcgcgg tcccgtcgtc gagttccaga tcgtccgcgt
     4021 cgcgcggggc gatcttgaat gcgtcgtagg tgtagacgag gatgccggtt atccgtttga
     4081 accgcgtgcc ggtcgagcgc tcgccgggat aatagaagag tttcttgtcc accacgagcc
     4141 cgccggtcac ctcccactgg ccgtggccga ggtcctcgtt ggtgaccgtg acatcgtgca
     4201 cctcgatgag cacgctctcc cactgctcgg ccgccgcgcc gtgggcggtc gtcgggagcc
     4261 actcgtcgtt gctcaacgcg aacggcgtcg cgatgctcga cgggtcggcg atgagcgcgg
     4321 gggccgggat gtcggcggaa ctgagtttgc cgacggtcgc gttctcgacc tgcgaggcgc
     4381 cgtagtactc cttgtaggtg ccgcttatct caagcaggtc gccacgggcg taggcgtcga
     4441 ccgcggcggg tgttttgatg aagacataga tgccgctcca cggcagggcg gtgtcgagcc
     4501 cggcctgact gacgaagagt cccttgatgc cggtcggcgc gtactcggta tcgagcacat
     4561 attccaccgc cgtcacgacc gcttcgaacg agaccggcga atcgaccgcg accgtcccct
     4621 gctggatatc gccgatgacg ccgagagaga ccggcgcgtc gtcggggacc accgtgtcgg
     4681 tgtcggacac cgcgtcatcc tgtaggggcg ggccgtcgtc ggacagagcg gccggttggt
     4741 cgccgtcgat cgcgatgtcg tcaccgtcgc ctgtggcctc gcggtcggtg aaccaactct
     4801 tgtcggggtc gaggttacag ccggcgcacc acagaagccc cgtgatgagt aaccagcgat
     4861 acgcaagaac cagcatggac accctctcca gcataacggt cacgccccat gctacgataa
     4921 aagggacgat aagtaaagtc cttaccgaca aaggaaagat gccgccgttt ccttgcaaaa
     4981 aaaacctgtt tcgttatcct tatctggcgg aaggtccccg gaaaagttct ttatgcgacc
     5041 gaggataggg aggaacggat gaaagcgttt cttgtgtctc tagcgttctg ttcggcgttg
     5101 ctgtgccttg cctgcgtcga ggacgactgc gtctactgcg gcaaggatgt gtcgggcggc
     5161 gttacctacg atgccgatgc ccataaaccg ctggtggaca aggataccgt gaaaccggac
     5221 ggtcagaccg tcgccgatca ggacacggcg gagcccgacg aggaactctc cgatgcggac
     5281 acgctggtgc ccgacgataa cttcgtcgtc gccgaccgca tggtgctcgt cccgggcgac
     5341 accttcaaca tggggtgtct cgatgtcgag gacccggcct gcgccgagac ccactcctcg
     5401 ccgcgccacg aggtgacgct tcccgatttc gagatagaca tctacgaggt gaccaagcag
     5461 gaattcgagg cctgcatcaa ggcgggtggc tgcaagaacg atgacggcga gccggtccag
     5521 tatgtcgcct atgacgccga ccagaacgac tactgcgtca tctacggcgt gtacgataag
     5581 gaccttccgg tcaactgcgt cagttggtac ggggcgcgcg cctactgcga gcaggccgac
     5641 gaggaggagc gtctcggtga gcatggcgag gaagaccgag a
//
LOCUS       DAOQUE010000140         5673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15660_length_5673_cov_4.514596, whole genome shotgun sequence.
ACCESSION   DAOQUE010000140 DAOQUE010000000
VERSION     DAOQUE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15660_length_5673_cov_4.514596"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PKH10_05625"
     CDS             complement(<1..525)
                     /locus_tag="PKH10_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27648.1"
                     /translation="MELPRYTLAVLFVLLLTVSCGPSDKPAADTEPATDTDEVTGETD
                     APADADKALPETDTVTGDDAGLDGDIVPDGDTKIDDDVTKDSDAAPDVDEIWANADDD
                     GDGIPNGKECAENPCEDTDDDGIADYLDEDTDGDGIPDSVEAPGGIPIDSDSDGTPNF
                     KDTDSDGDGIPDSVE"
     gene            complement(566..2578)
                     /locus_tag="PKH10_05630"
     CDS             complement(566..2578)
                     /locus_tag="PKH10_05630"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27649.1"
                     /translation="MKKTTVTAIILATMLALFLLNCGDESPNDPGNDDVAGSDDDAND
                     DDAKTDTGGTPDTKVDGDAPVTEGGGDYPPDTDNMTAWNELDDDSDGVKNGIEGTADT
                     DGDGTPNYLDLDSDGDGLPDGTEAPSGIPVDTDNDGPPDFLDTDSDNDGVLDGVEGTG
                     DADSDGIPNYRDPDADGDGILDGIECSEQPCTDTDTDTTPDYLDSDSDGDGVPDLYEG
                     NKDGDSDGAPNYRDTDSDGDGIPDNEECPATPCADSDNDGFPDFKDKDSDNDGLTDAK
                     ELEIGTDPHKADTDDDGVDDNTENAFGSDPKDPTSSIPENAYYYILPYNAPAVQDSLD
                     FQTNIQKADIMIMVDLSGSMGGEHANLKTGINTVVIDGIKAQIPGVAFSLAKFGTLED
                     DPYILAQGITTDAAAVQSAVNTIAECGGSSEAAYEALYQAMTGQGVTYECDGGGCNII
                     MAASTIPTSVPGWRDEALPIVIMCTDEELQDYASSCDTHSKAEALAAMNAINAKFIGI
                     NSGGGSTLTSNFQEISTSTGSLDGSGNAFNYTINSDGTGLSQQVVDAVVALSKGIQID
                     VNTIVKSVANDESVDTSAFVKAVVPNTADPINGFDGKNETTFFKVKPGTVVTFDVTFQ
                     NTTYDNQTTETKLFVANINVMGAGTLLDTRQVFILVPGIVDNGGDD"
     gene            2804..3913
                     /gene="serC"
                     /locus_tag="PKH10_05635"
     CDS             2804..3913
                     /gene="serC"
                     /locus_tag="PKH10_05635"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNT27650.1"
                     /translation="MEKRVHNFYAGPAALPTEVMKEAQAELLNFMGTGMSVMEISHRS
                     KEFDKVIQEAEADMLKILGLSSEQYTVLWLGGGATHQFIMLPMNTMEKTDTADYIVTG
                     QWAERAHEEGAYWGNAHIVATSKGEAKPYCRLPEIKKENMSDNAKYLHFTTNNTIYGT
                     QFWKFPEPKAGIPLISDMSSDFLSRTFDASKFDMIYAGAQKNIGPAGVTAVVIKKAFA
                     EKYCKKKLPKTLDYNVQIKKQSLFNTPPCFNIYMVGKVMKWILGKGGLAAVEAMNRKK
                     AELIYGAIDAHPEFYKGYVTVKEHRSWMNVNFNLPTPELDEKFVKEAKAEDMVGLKGY
                     RDLGGIRASLYNATTVESVEKLVAFMDKFYKANNK"
     gene            4041..4373
                     /locus_tag="PKH10_05640"
     CDS             4041..4373
                     /locus_tag="PKH10_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNT27651.1"
                     /translation="MNVQFKKGVLDLCVLAVVAEGDCYGYELVKRISENIEIAEGTIY
                     PLLRKFQNEGYCETYLVESSGGPPRKYYRLTPAGRRMRDQLITEWTKFSKSVDTIITS
                     NQRGRSNG"
     gene            4366..4944
                     /locus_tag="PKH10_05645"
     CDS             4366..4944
                     /locus_tag="PKH10_05645"
                     /inference="COORDINATES: protein motif:HMM:NF019618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HNT27652.1"
                     /translation="MDKRQYLARLRDSLAGLPLPEVEDIIRDHEEHFRIGAESGKSEA
                     DIAVSLGMPEEIARQYRSETPAGVPVVEGPVDRTKMWVAGAILIALNVTVLATPVVSL
                     FWLWLASWIVAWVAAILGAVIIVAGLVTIPVPILNFFASPFLVFFGTGALLLGIGGTM
                     LLSVVTPLAVRIIKWWVNFNIDLARRAGGVHA"
     gene            4937..>5673
                     /locus_tag="PKH10_05650"
     CDS             4937..>5673
                     /locus_tag="PKH10_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNT27653.1"
                     /translation="MRRKVFSALICLAFSGVFFAVAFGMFFNAAQASTALNQSGLAPL
                     FNNVTGLIREGMEPMRGCSVADFEGELKSIRISTITAGVRIETITDAMPVLDTDAGVN
                     CRRTGDTVEIRVPPFSLRKRHRSVNLRIPPTDSGDLTLFLPPEKRYDLEIKTISGDIH
                     AGDIAVGKLAVESKSGDITVRGLIGDLTASTVSGDITAELKELTLFAAAVSVSGDIRL
                     RLPGNESYDITSETVSGEIQMKASEEKG"
BASE COUNT         1066 a         1687 c         1718 g         1202 t
ORIGIN      
        1 ctcgacgctg tcgggaatgc cgtcgccgtc gctatcggta tccttgaagt tgggggtgcc
       61 gtcgctgtcg gagtctatcg gtatgccgcc cggcgcctcg acgctatcgg gtatcccgtc
      121 gccgtccgtg tcctcgtcga gatagtcggc gatgccgtca tcgtccgtat cctcgcacgg
      181 gttctccgca cactccttcc cgttcgggat cccgtcgccg tcgtcgtcgg cgttcgccca
      241 tatctcgtcg acatcgggcg cggcgtcgga gtctttcgtc acatcgtcgt cgatcttcgt
      301 gtcaccgtcc ggaacgatgt cgccatcaag gccggcatcg tctcccgtca cggtgtcggt
      361 ctccggaagc gctttgtcgg catcggcggg cgcgtcggtc tcgccggtca cctcgtcggt
      421 gtcggtcgcc ggctccgtgt cggccgccgg cttatcgctc ggcccgcagg aaacggtcag
      481 aagcagaaca aaaagaacgg caagcgtata tctcggcagt tccatgcgac tggctccctt
      541 gctgaacgga ccttttcctc tatccctagt cgtcgccgcc gttgtcaacg atgcccggca
      601 cgaggataaa gacctgccgc gtatcgagca gggtaccggc gcccatgaca ttgatgttgg
      661 cgacaaagag tttcgtctcg gtcgtctggt tgtcgtaggt cgtgttctgg aaggtgacat
      721 cgaaggtaac aacggtgccc ggcttcacct tgaagaatgt ggtttcgttc ttcccgtcga
      781 agccgttgat aggatcggcg gtgttcggga cgaccgcctt aacgaacgct gaggtgtcga
      841 cgctctcgtc gttcgcgacc gacttgacga tggtgttgac atctatctgt atccccttcg
      901 agagagcgac gaccgcgtcg acgacctgtt gcgacagccc ggtaccgtcg gagttgatgg
      961 tgtagttgaa cgcgttgccg ctcccgtcaa gcgagccggt actggtcgat atctcttgga
     1021 agttgctcgt cagcgtgctg cccccgcccg aattgatgcc gatgaatttc gcgtttatcg
     1081 cgttcatcgc cgcgagcgcc tccgccttgg aatgggtatc acaactactc gcgtagtcct
     1141 gcagttcctc gtcggtgcac atgatgacga tgggaagcgc ctcgtcgcgc cagccgggaa
     1201 cgcttgtcgg gatcgtgctt gccgccatga tgatattgca accgccgccg tcgcattcat
     1261 aggtgacgcc ctgaccggtc atcgcctgat agagcgcctc gtaagccgcc tcggacgaac
     1321 cgccgcattc ggctatcgtg ttgacagcac tctggaccgc cgccgcatcg gtcgttatcc
     1381 cttgggcgag tatatagggg tcgtcctcga gggtcccgaa cttcgcgagg ctgaacgcca
     1441 cgcccggtat ctgcgccttg atgccatcga tgacgaccgt attgatgccg gtcttgagat
     1501 tggcatgctc gccccccatc gaaccggaga gatcgaccat gatcatgatg tcggccttct
     1561 ggatgttggt ctggaagtcg agcgagtcct gcaccgccgg ggcgttgtag ggcaggatat
     1621 agtaataggc attctcgggg atgctgctcg tcggatcttt cgggtcgctc ccgaaagcgt
     1681 tctcggtgtt gtcgtccacc ccgtcgtcat cggtgtcggc cttgtgcggg tcggtgccta
     1741 tctcgagttc cttggcatcg gtcagcccat cgttgtcgct gtccttgtcc ttgaaatcgg
     1801 ggaagccgtc attgtccgaa tcggcgcagg gggtcgcagg acattcctcg ttgtcgggga
     1861 tgccgtcgcc gtctgaatct gtgtcgcggt agttcggcgc gccgtcgctg tcgccgtcct
     1921 tgttcccttc gtagagatcg ggcacgccgt cgccgtcgct gtcgctgtcg aggtagtcgg
     1981 gggtcgtgtc ggtgtcggta tcggtacagg gctgttcgga acactcgatg ccgtcgagga
     2041 tgccatcgcc atcggcgtcg gggtcgcggt agttcggtat cccatcgctg tcggcgtcgc
     2101 cggtcccttc gacgccgtcg agaacgccgt cgttatcgct atcggtgtcg aggaagtcgg
     2161 ggggaccgtc gttgtcggtg tcgaccggga taccgctcgg cgcttcggtg ccatcgggca
     2221 gaccgtcacc gtcgctgtca aggtcgagat agttgggcgt cccatcgccg tcggtgtcgg
     2281 cggtcccctc gatgccgttc ttgaccccgt cactatcatc gtcgagttcg ttccatgcgg
     2341 tcatgttgtc ggtgtcgggc gggtagtcgc cgcccccctc ggtgaccggg gcatcgccgt
     2401 cgacctttgt gtcgggcgtt cctcccgtgt cggtcttggc gtcatcgtcg ttcgcgtcgt
     2461 cgtcactgcc ggcgacatcg tcgttcccgg ggtcattcgg gctctcatcg ccgcagttca
     2521 acaagaacag cgccagcatc gtcgccagga tgatcgccgt aacggtcgtt ttcttcatgg
     2581 tatcctcctt ctggagatag ctccttttgt gtagttaccc gctcgcatgg ggattattat
     2641 aaaaatcact ttggtaaaag caaccataaa aaatcccgcg ggcgggatga ccgaccgaaa
     2701 aatgttgcaa aagggtatcg aacacgctac aattgaccga aagataggcg actcaggcaa
     2761 ggtgcggata gcgtaacttc ttttaatttt tggaggtcat cccatggaaa aacgggttca
     2821 caatttttat gcgggcccgg cggcgttgcc gaccgaggtc atgaaagagg cgcaggccga
     2881 actgctcaac ttcatgggca ccggcatgag cgtcatggag atcagtcacc ggtccaagga
     2941 gttcgataag gtcattcagg aggccgaggc cgacatgctg aagatcctcg ggctctcatc
     3001 ggagcagtac accgtgctgt ggctgggcgg cggggcgacc caccagttca tcatgctccc
     3061 gatgaacacg atggaaaaga ccgacaccgc cgactacatc gtgaccggtc aatgggccga
     3121 gcgggcccac gaagagggcg cctactgggg caatgcccat atcgtggcca cctcgaaggg
     3181 cgaggcgaag ccctactgcc gcctccccga gataaagaag gagaatatgt ccgacaacgc
     3241 gaagtatctc cacttcacga ccaacaacac catctacggc acccagttct ggaaattccc
     3301 cgaaccgaag gcgggcatcc cgctgatatc ggatatgtcg agcgacttcc tgtcgcgcac
     3361 cttcgacgcg tcgaagttcg acatgatcta tgccggcgcc cagaagaaca tcggccccgc
     3421 cggggtcacc gcagtcgtca tcaagaaggc gttcgccgag aaatactgca agaaaaaact
     3481 gcccaaaacg ctcgactata atgtccagat aaagaagcag tcgctcttca acaccccccc
     3541 ctgtttcaac atctacatgg tcggcaaggt gatgaagtgg atcctgggca agggcggcct
     3601 cgccgccgtc gaggcgatga accgcaagaa ggcggaactc atctacggcg ccatcgacgc
     3661 tcatcccgag ttctacaagg gctatgtcac cgtcaaggag caccggtcgt ggatgaatgt
     3721 gaacttcaac ctgccgacgc cggaactcga cgagaagttc gtcaaggagg cgaaggccga
     3781 agatatggtc gggctcaagg gataccgcga cctcggcggc atccgcgcca gtctctacaa
     3841 cgcgacgacg gtcgaatcgg tcgagaaact ggtcgcgttc atggacaagt tctacaaggc
     3901 caacaacaag taggccgccc aacccataac tcgcattgaa ttctacataa tacaaatagg
     3961 ttaataactt ttttcgccaa ctaccttgct ttatacggta gcgtgctgta tgttgtaccg
     4021 tgcttagtgt aggaggcagc atgaatgttc aattcaagaa aggggtcctt gacctgtgcg
     4081 tgctggcggt ggtggccgag ggcgactgct acggctacga actggtcaag cggatctcgg
     4141 agaacatcga gatcgccgag gggaccatct atccgctcct gcggaagttc cagaacgagg
     4201 ggtactgcga gacctacctc gtcgaatcga gcggcggacc gccgcgcaaa tattaccggc
     4261 tcaccccggc ggggcgccgg atgcgcgatc aactcatcac cgaatggacg aagttctcga
     4321 aaagcgtcga cacgataata accagcaacc aacgggggcg cagcaatgga taaaagacag
     4381 tatctggcac ggcttcgcga cagccttgcg gggcttcccc tgccggaggt cgaggatatc
     4441 atccgcgacc acgaggaaca tttccgcatc ggcgccgaat cgggcaagtc ggaggccgat
     4501 atcgccgtgt cgctcggtat gcccgaggag atcgcgcggc agtaccgcag tgagacaccc
     4561 gccggtgtcc cggtcgtcga gggaccggtg gaccgcacga agatgtgggt ggcgggggcg
     4621 atcctcatcg ccctcaatgt gacggtcctc gcgacaccgg tcgtgtcgct tttttggttg
     4681 tggctcgcct cgtggatcgt cgcgtgggtc gcggcgatac tgggagcggt catcatcgtc
     4741 gcggggctgg tgaccatacc ggtccctata cttaacttct tcgcctcgcc cttcctcgtt
     4801 ttcttcggca ccggggctct cctgctgggg atcggcggca cgatgctgtt gtccgtggtg
     4861 accccgctcg ccgtgaggat aatcaaatgg tgggtcaact tcaatatcga tcttgcccgc
     4921 cgggcgggag gggtccatgc gtaggaaagt gttctccgcg ctcatctgtc tcgccttttc
     4981 gggtgtcttt ttcgccgtcg ccttcgggat gttcttcaat gcggcgcagg catccaccgc
     5041 cctcaaccag agcgggctcg cacctctttt caataatgtg acggggctta tccgcgaagg
     5101 gatggagccg atgcgcgggt gttcggtcgc cgactttgaa ggcgagctca agagcatccg
     5161 catctccacc atcaccgccg gggtacggat cgagaccatc accgacgcca tgccggtgct
     5221 cgacaccgac gccggggtca actgtcgccg caccggcgac acggtcgaga tacgggtccc
     5281 gccgttcagt ctgcgaaaac gccaccgttc ggtcaatctg cgcattccgc cgaccgactc
     5341 gggcgacctg acgctcttcc tgccaccgga gaaacggtac gacctggaga taaagacgat
     5401 atcgggcgat attcacgccg gggatatcgc ggtcgggaaa ctggcggtcg agagtaaatc
     5461 gggcgacatc accgtcaggg gactcatcgg cgatctgacc gcatcgaccg tatcgggcga
     5521 catcaccgcc gaactgaagg aactgacgct cttcgccgcc gccgtgtcgg tatcgggcga
     5581 catacggctc cgtctgccgg gcaacgagtc ctacgatata acctccgaaa cggtatcagg
     5641 cgagatacag atgaaagcgt cagaagagaa agg
//
LOCUS       DAOQUE010000141         5667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15680_length_5667_cov_4.544726, whole genome shotgun sequence.
ACCESSION   DAOQUE010000141 DAOQUE010000000
VERSION     DAOQUE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15680_length_5667_cov_4.544726"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /locus_tag="PKH10_05655"
     CDS             <1..1703
                     /locus_tag="PKH10_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNT27654.1"
                     /translation="EEYRILPHEKTELFVAKRGYYEAQYRDRFKKDPFVTDLVLFMCR
                     EEGVTVEEETLLRLVAGRAGCDYVVPDPLKIDSQLVTSIISLPYGRSHFVLPLYEEDG
                     AVVIAMADPFRMEIVDQLRSAAGRPVVPVVASPSSIEKILGDLFGFKRSIKAAAKERT
                     RDAYASIEQLTSIVASGQLDDKHIVNAVDYMLRYAVEQGASDIHIEPKRDETVIRFRL
                     DGVMQTIYNFPIQAHAPFLSRLKMLARMDIAEKRRPQDGRIKLLLDEAQEVELRASTV
                     PAVYGEKMVLRILESGHFVRPLEEIGFEEAQLDAWRGGMQKGYGMLLVTGPTGSGKST
                     TLYSTLKELAKPDVNIVSIEDPIEIVIEDINQIGVNTKAGITFSSALRHILRQDPDII
                     MVGEIRDKETADNAVQAALTGHLVFSTLHTNDTASTVERLIDLGVEPFLLSSMLNGVL
                     AQRLVRKLCTACSFDRDMTEEEKIALKLPIESRYRIKGSEGCVKCRYTGYKGRTAIME
                     YMPVSGKIGRLIANRATTDEIRQNAVLDGMVTLRDAAIRKLAGGATSFEEIVRALYFE
                     "
     gene            complement(1734..2948)
                     /locus_tag="PKH10_05660"
                     /pseudo
     CDS             complement(1734..2948)
                     /locus_tag="PKH10_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(3066..4175)
                     /locus_tag="PKH10_05665"
     CDS             complement(3066..4175)
                     /locus_tag="PKH10_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017756673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT27655.1"
                     /translation="MTAHDEGSMLETILGYMSKIEGVRKIDAVLILLSEMGRVLVNAD
                     RCTLWLLDTQKKELWTKVAHGIDRVHMPAGTGIAGAAVGSGETVIVNDPYRDPRFNQE
                     IDRKTGYTTRSILVVPIKNSRGEVLGAFQTINKQTAAGRFEESDIKYLKLASTYAGEA
                     IEAAKLNEEIEETQRDVIFALAEAGETRSKETGNHVQRVSEYCRSLALLACLPKKEAE
                     LLKLAAPMHDIGKIAIPDAILLKPGRLEPEEFAVMKTHAESGYTILKGSSRPILKAAA
                     TVAREHHERYDGTGYPRGLKGKEIHIFGRICAIADVFDALGTDRVYKKAWPIEKIVEE
                     FTAQRGAQFDPALIDLFLGRLDEFTAIRERFRDEV"
     gene            4271..>5667
                     /locus_tag="PKH10_05670"
     CDS             4271..>5667
                     /locus_tag="PKH10_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNT27656.1"
                     /translation="MRAWSLLFVIPLLFSCGGKSASAVCAGISVTTDGNATEELYLRA
                     SFNNWSLSTPMSFRAGRWEVELSLPPGDHAYAFYDRKKEEWRLDEENPLARYDNGKRY
                     SRLIVPDCRYPRLEVVDRPVADDSSLSLTVRYLPGASGAGPDLKKSMVILSGEAVTPS
                     YDGTAKLFTLAANGLAPGKYTALFTIRDTDGFAATPLFVPVWVEQEPFDWRDAVIYQI
                     MTDRFVNGDPVNDGPVAGVDKKANWQGGDLRGIIEKIEENYFTDLGVTALWLSSPLRN
                     TQGAWKGMGGDTRLYAAYHSYWPVATGWSDLTPLPGLASPVEPHFGDEADLRELVKKA
                     HDRGIRVLFDIVPNHVHAESWLWETYRYDDWFNEAPAGLPRNALGGYTCGWERPEECW
                     FTDYLPDIDHRNAAAQDALIEHYLWLARTFDADGFRVDAVRLMNRDLTVTLRYFIERE
                     AAAGGTPFFMVGETFT"
BASE COUNT         1003 a         1742 c         1806 g         1116 t
ORIGIN      
        1 tggaggaata tcggatcctg ccgcacgaga agacggaact gttcgtcgcc aagcgcgggt
       61 actacgaggc ccagtatcgc gaccgcttca aaaaagaccc gttcgtgacc gaccttgtgc
      121 tcttcatgtg ccgcgaggag ggggtgacgg tcgaggagga gacgctcctg cggctcgtcg
      181 cgggccgcgc cggctgcgac tatgtcgtgc ccgacccgct caagatagat tcgcaactgg
      241 tcacctcgat catatcgctg ccctacggcc gttcccattt cgtcctcccg ctctatgagg
      301 aggatggcgc cgtcgtcatc gcgatggccg acccgttcag gatggagatc gtcgatcagt
      361 tgcggtcggc cgccggtcgg ccggtcgtgc cggtggtcgc ttcgccgtcg tccatagaga
      421 agatactcgg cgacctgttc ggcttcaagc ggtccatcaa ggcggccgcc aaggagcgga
      481 cccgcgatgc ctacgcctcg atagagcagt tgacgagcat cgtcgcgagc ggtcaactcg
      541 acgacaaaca catcgtcaac gcggtcgact acatgctccg gtacgccgtg gagcaggggg
      601 cgagcgacat ccacatcgag ccgaagcgcg acgagaccgt catccggttc cggctcgacg
      661 gcgtgatgca gacgatatac aacttcccga tacaggcgca cgccccgttc ctgtcgcggc
      721 tcaagatgct cgcccgcatg gacatcgccg agaagcggcg cccgcaggac ggccgcatca
      781 aactcctgct cgacgaggcg caggaggtcg aactgcgcgc gagcaccgtg cccgccgtgt
      841 acggcgagaa gatggtcctg cgcatcctcg aatcgggcca cttcgtgcgg ccgctcgaag
      901 agatagggtt cgaagaggcg caactggatg cgtggcgcgg cggcatgcag aaagggtacg
      961 gcatgctact cgtcaccggc ccgaccggga gcggtaagtc gaccacgctc tactcgacgc
     1021 tcaaggaact cgccaagccc gatgtcaaca tcgtctccat cgaggacccc atcgagatcg
     1081 tgatagagga catcaaccag atcggggtga acaccaaggc gggtatcacc ttctcgtcgg
     1141 cgctccgcca catcctgcgt caggaccccg acatcatcat ggtcggcgag atacgcgaca
     1201 aggagacggc cgacaacgcg gtgcaggcgg cgctcaccgg gcacctcgtg ttctcgacgc
     1261 tccacaccaa cgacaccgcg agcacggtgg agcggctcat cgacctcggc gtcgagccgt
     1321 tcctcctctc ctcgatgctc aacggcgtgc tcgcccagcg gctggtgcgg aaactctgca
     1381 ccgcctgttc gttcgaccgc gacatgaccg aagaggagaa gatcgccctg aaactcccca
     1441 tcgagagccg ttaccgcatc aagggctccg aggggtgcgt caagtgccgt tacaccggct
     1501 acaaggggcg caccgccatc atggagtaca tgccggtgtc gggcaagata gggcggctca
     1561 tcgcgaaccg cgcgacgacc gacgagatac ggcagaacgc ggtgctcgac ggcatggtca
     1621 ccctgcgtga cgcggcgata cggaaactcg cgggcggcgc gacatcgttc gaagagatcg
     1681 tgcgggcgct ctatttcgag taagatacct ccccggccgg ggaaaagagg cggtcagccc
     1741 ttcatcgctt cctttatgac ctcggcgaga cgccggatgc cttcgacatt cttctctttc
     1801 gaggcgaacg agaagttgag ccgcatcgtg tcgcgtcccg acccgtcgca gtggaaaacg
     1861 ctgccgagca cataggcgac attcttctct atcgccttgg ggaacagttt ctcggcgtcc
     1921 attccgttgg gcagggtgac gaacaggaac agcccgccct cagggcaggt ccacgagacc
     1981 cccttcggca tgtgttcctt gagggcggcg agcatcgcgt cgcgtttctc gcggtacatc
     2041 gcgatgaccc gctggaggtt ggcgtcgaaa tgtccctgat tcaagtagag cgccccgatc
     2101 ttctggacga acgaggaggt gcagaggtcg gccgcctgct tgacggtcac gaacttgttg
     2161 aggaggtcct tgctgtcgat gattcagccg atgcggaagc cggggacgaa tatcttcgag
     2221 aaggtgccga gcacgacgac ctgttctttg ccgtcgagtt tgtagagcat cggcgggttc
     2281 ttgccctcga agcgcagttc gcggtacggg ctgtcctcga cgacgagcac atcgtactcg
     2341 tgggcgagcg ccagtatctc tttgcgtcgc gattcgggca tcgtgatgcc ggccgggttc
     2401 tggaaatcgg ggacggtata gatgaatttc ggctttttcc ccgccttttt cagttcctcg
     2461 agtttcgcct tcaaaaggtc ggtgcgcata ccgttctcgt cgagtttgat gccgtgcatc
     2521 tcggccccgt aggccgaaaa ggcctgcagg ccgccgagat aggtggggag cccgcaaacg
     2581 atcgcgtcgc ccgggtcgat aaggacgcga ccgaggaagt cgagcgcctg ctgggaggag
     2641 gtcgtgatga tgatgttctc catcgccagt tccagccctt cgtccttgcg gtatttctcg
     2701 ataagcgcct tgcgcaggcc gttgtcgcct tcggtcatgc tgtactggca cgcggcggcg
     2761 ccctcttttt cgagcacctc gaccgagagt ttcttgagga tgtcgagcgg gaacgactcg
     2821 ggggcgggca gcccgccggc gaatgagatg atctcggggc gctgggtcag tttcagcagt
     2881 tcgcggatgg ccgatttctt ggcgctcttc gcgctgcggg agaggatgtt ctgaaggtct
     2941 ttgatcatga gcgtctcctt ttatgaacag ttgaggaaca ctgctttcaa agagtccggg
     3001 cacctcaagc ctatgccccg agggagagga tgtcaaaaaa ataagaggtg atcgtcgcgg
     3061 gccggtcaga cctcgtcgcg gaaccgttcg cggatggcgg tgaactcgtc aaggcggccg
     3121 aggaagaggt ctatcagtgc cgggtcgaac tgggcgccgc gctgggccgt gaattcttcg
     3181 actatcttct cgatgggcca cgccttttta taaacgcggt cggtgccgag cgcgtcgaac
     3241 acatcggcga tggcgcagat gcggccgaat atgtgtatct ccttcccttt gagtccgcgc
     3301 gggtaccctg tgccgtcgta ccgttcgtgg tgttcgcggg cgacggtcgc cgccgccttc
     3361 aggatggggc gcgacgagcc tttgagtatc gtgtagccgc tctcggcgtg ggtcttcatg
     3421 acggcgaatt cctccggttc gagccgtccc ggcttgagca gtatcgcgtc gggtatcgcg
     3481 atcttgccga tgtcgtgcat gggagcggcg agtttgagca gttcggcctc ctttttgggc
     3541 agacaggcga ggagcgcgag cgaccggcag tattccgata cccgttggac atggttgccg
     3601 gtctccttcg accgggtctc tcccgcctcg gcgagcgcga agatgacatc gcgctgggtc
     3661 tcctctatct cctcgttgag tttcgccgcc tctatcgcct cgccggcgta ggtcgaggca
     3721 agtttgaggt acttgatgtc gctttcctcg aaccggcctg cggcggtctg cttgttgatg
     3781 gtctggaagg cgccgagcac ctcgccgcgg ctgttcttta tcgggaccac gaggatgctg
     3841 cgggtggtgt agccggtctt ccggtctatc tcctgattga agcgggggtc gcggtacggg
     3901 tcattgacga tgaccgtttc gccgcttccg acggcggccc cggcaatgcc ggtgccggcc
     3961 ggcatgtgga cgcggtcgat gccgtgcgcc accttggtcc agagttcctt cttctgcgtg
     4021 tcgagcagcc acagcgtaca gcggtcggcg ttgacgagca cgcgtcccat ctccgaaaga
     4081 agtatgagca ccgcatctat cttgcggacc ccctctatct tcgacatgta gccgagtatc
     4141 gtctccagca tcgatccttc gtcgtgagcg gtcatgggtc ccctcccgta aagaagaccc
     4201 atcgtatcgc gggtcggttg gcttttcaag attttatcgt tatactcgat cgtccgatcg
     4261 gagggtcccg atgcgcgcat ggtccctgct gtttgtcatc ccgttgcttt tttcctgcgg
     4321 ggggaaaagc gcttccgccg tttgcgcggg gatatccgtc accaccgacg ggaacgccac
     4381 cgaggaactc tacctgcgcg cctcgttcaa taactggtcg ctctcgacgc cgatgagttt
     4441 ccgcgctggt cggtgggagg tcgaactgtc cctgccgccg ggtgaccacg cctacgcttt
     4501 ttacgaccgg aaaaaggagg agtggcggct cgacgaagaa aatccgcttg cccggtacga
     4561 caacggcaaa cgatacagcc ggctcatcgt tcccgactgc cgctacccgc gcctcgaggt
     4621 ggtcgatcgt ccggtcgccg acgattcgtc gctctcgctg acggtccgct accttcccgg
     4681 ggcgagtggc gcgggccctg acctgaagaa gagcatggtg atcctttctg gggaggcggt
     4741 caccccttcg tatgacggca ccgcgaaact tttcaccctt gccgcgaacg gtcttgcgcc
     4801 cgggaagtac accgccctgt tcacgatccg cgacaccgac gggttcgccg cgacaccgct
     4861 gttcgtgcct gtctgggtcg aacaggagcc gttcgactgg cgcgacgcgg tgatctacca
     4921 gatcatgacc gaccgcttcg tgaacggcga tcccgtgaac gacggcccgg tcgccggggt
     4981 cgacaagaag gcgaactggc aggggggcga cctgcgcggt atcatcgaaa agatagagga
     5041 gaactacttc accgatctgg gcgtcaccgc actttggctt tcatcgccgc tccgcaacac
     5101 gcagggggcg tggaagggaa tgggaggcga cacgcggctc tacgccgcct atcattccta
     5161 ttggccggta gcgaccggat ggagcgatct tacgcctctg ccggggctgg cgtcgcccgt
     5221 cgagccgcac ttcggcgatg aagcggacct gcgcgaactg gtgaaaaagg cgcacgaccg
     5281 cggcatccgc gtactgttcg atatcgttcc caaccatgtc cacgccgaaa gctggctctg
     5341 ggagacctac cgctacgacg attggttcaa cgaggcgccc gccggtctgc cgcgcaacgc
     5401 gctggggggc tatacctgcg gctgggaacg gcccgaggag tgctggttca ccgactatct
     5461 gcccgacatc gaccaccgca acgcggcggc gcaggacgcc ctcatcgagc attacctgtg
     5521 gctcgcgcgg accttcgacg cagacgggtt ccgcgtcgat gcggtacggc tgatgaaccg
     5581 cgacctcacc gtgacgctcc gttacttcat cgagcgcgag gcggcggcgg gaggcacccc
     5641 gttcttcatg gtcggcgaga ccttcac
//
LOCUS       DAOQUE010000142         5654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15722_length_5654_cov_4.564565, whole genome shotgun sequence.
ACCESSION   DAOQUE010000142 DAOQUE010000000
VERSION     DAOQUE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15722_length_5654_cov_4.564565"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /gene="trmD"
                     /locus_tag="PKH10_05675"
     CDS             complement(<1..599)
                     /gene="trmD"
                     /locus_tag="PKH10_05675"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNT27657.1"
                     /translation="MRIDILTLFPEYFDGPLSVSMMKRAVEKGLADFHLHQIRDHGKG
                     KRRQVDDAPYGGGAGLVMMAEILLNAYESVPKEKRCRSFLLSPRGKKFDQNMARELAA
                     GCDQIVLIAGHYEGIDQRFMDLTGIEELSLGDFVLTGGEPAAAAVVDAVVRLIPGVLG
                     NEGSIEQESFSLPLLEHDHYTMPREFRGLKVPKVLFSGD"
     gene            778..1785
                     /locus_tag="PKH10_05680"
     CDS             778..1785
                     /locus_tag="PKH10_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27658.1"
                     /translation="MNKFTLVLLSALIALTAAVSCKFEPPIEEKLNAYIQSYLYAVRD
                     GKTDEVYNTMLSAKMKEVFTLEDWNEYVSRVYKGKIGQELRTAYATIVKEKSTGEFIE
                     ATGRTSNMGRMGNVMNQDETLIFQVHVVNENGQFKVEQSEDMSALQEEKDAQKRLDEL
                     LNNYQGLVKLEDVMVEPLPQRPGWALVSGTILNASSDLDMVRVGVKIRLKDSAGDIIY
                     AQKFFPVLDVRYEGLRTSVLPGDSKLFQTPIKDIPPEWDKNQPPDFQFYLIDGTVISE
                     EDLKKEIVDRDKMRKLVEETRKGDEEARRQMNEQWDKEKRLKAKIKAMQERSKAKEEK
                     K"
     gene            1879..2187
                     /locus_tag="PKH10_05685"
     CDS             1879..2187
                     /locus_tag="PKH10_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27659.1"
                     /translation="MTLLAVLLFLSAEAFADRDLYIIWLPSNAVKKAEKVYTIPLDLD
                     GAVKKVKQRMAQDPLIRSDVLLNEDGFRIHAFYNLRQTGAWRKMYLIEEDSRVTARFF
                     "
     gene            2190..2263
                     /locus_tag="PKH10_05690"
     tRNA            2190..2263
                     /locus_tag="PKH10_05690"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2222..2224,aa:Gln,seq:ctg)
     gene            2277..2351
                     /locus_tag="PKH10_05695"
     tRNA            2277..2351
                     /locus_tag="PKH10_05695"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2310..2312,aa:Glu,seq:ctc)
     gene            complement(2365..>5654)
                     /locus_tag="PKH10_05700"
     CDS             complement(2365..>5654)
                     /locus_tag="PKH10_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT27660.1"
                     /translation="LVSVEGVTVLDTGLGNGEFLVTGDLRVDDTLFTYTFPPVGTLYE
                     ALTGIMHYTWNNAKLEPRSLDDMVVATATDDGMATDDAVSTDADQSDPTDQSDQSDQD
                     AEVPDTDAGGEEEIFCAELIPPAEGLCAATAGSDDLLLRGVVLGHDKIYRGGEVLVGA
                     AGDIACVGCDCSAESGYAGATVVECAAGVISPALINAHDHLGYTYNAPGNWGTERFDH
                     RHDWRLGIRDHTKIPVPGSATVEQKQWGELRNVMAGTTAIAGSGGATGFLRNLDQNYF
                     NTEGLAFADVQYNTFPLGDSGGTLLASGCGYPSIDPATVLNNDCYLPHVSEGIDAEAR
                     NEFLCLSSSAGGGRDLTEANSAFIHLVGLKAQDALILAQNGTALIWSPRTNIALYGNT
                     APVTLYRTLGVNIGMGTDWTASGSMNMLRELKCADRLNVVFYGGFFTARDLWRMATIN
                     NATALRLAALTGSLQVGKVADLAIFDGAGYDDDPYLAIIDANVDGVALVLRGGEPLYG
                     DAAVMAVVPGGQSGCEALTVCTVPKSVCVERETGKTYAQLAAANSSAYALFSCGEPSG
                     EPTCTPLRTVSDDARPYNGPTMEDLDGDGIANGSDNCPMLFNPIRPVDNGVQGDADND
                     GVGDLCDPTPLGADPTVDPDDKDGDGIPNASDNCPYDANIDQLDDDGDGAGNVCDPCP
                     ESPNPMYGSCGIIPIYDLKDGTVLPGNKVETRGVVTALNGASFFLQVPEADHDPVIGY
                     AFSGIYVYSSLTAPAVGDIVTVVGTLTNYFGLLELEAISSITVESSGNDLPAPVIVDP
                     ADIATGGQYADDHNAVLVTVEGVTVLDNALGYGEFLVTGDLRVDSQLYFYTMPEVGVA
                     YDAITGVLFFAFDNSKILPRGADDMVKTPVTCGSDTCEDWEECQVDTCVLKTGRCDGD
                     GDCAEPTPTCNLATHYCEADGLTVLNGDFELWTNGTTPDSWTADSGVGVAKETTITHG
                     GSAASVKLTRNSTTNSNTDFVSDFVAIAENTAYNITFHYYDNDANGRGTLYYSFYDAS
                     KAQIGSTVYPGIYTTDQVDWQTITASPTSPAGSAYLTIGTRVYQQTGGTATGGSVYLD
                     DVTVTIP"
BASE COUNT         1068 a         1701 c         1782 g         1103 t
ORIGIN      
        1 tggtcgcccg agaagagcac cttgggcacc ttgagcccgc ggaactcgcg cggcatggtg
       61 tagtggtcgt gttcgaggag cgggagcgag aacgactcct gttcgatcga tccctcgttg
      121 ccgagcactc cggggatgag ccgcaccacc gcgtcgacca ccgccgccgc cgccggctcg
      181 ccgccggtga ggacgaaatc gccgagcgat agttcctcga tgccggtcag gtccatgaag
      241 cgctggtcta tcccttcgta gtggcccgcg atgaggacga tctggtcgca cccggccgcc
      301 agttcgcgcg ccatgttctg gtcgaatttc ttgccgcgcg gcgaaaggag gaacgagcgg
      361 catcgcttct ccttcggcac cgactcatag gcgttcagca gtatctccgc catcatgacg
      421 agtcccgcgc cgccgccgta cggggcgtca tccacctgac gacgtttccc cttgccgtgg
      481 tcgcgtatct ggtggagatg gaaatcggcg agccccttct cgaccgcccg cttcatcatg
      541 ctcacggaga gcggcccgtc gaaatactcg gggaacaatg tcaggatgtc gatcctcatg
      601 aggaggacta tcgccggagc gacccgcaaa gtcaagagtt ttcggcgcgc catatcacac
      661 gcccggcccg aaaataattt ctttactccg ttgaaatatc cgctatagtg atttctatcg
      721 ttgtctcttt gaaaggtgga actatttgaa tagtatggtc gtttttcgga ggaacgcatg
      781 aacaagttca ccctggtcct tctttcggcg ctcatcgcgc tcaccgcggc ggtatcgtgc
      841 aagttcgagc cgcccatcga agagaaactc aacgcctaca tccagagtta tctctacgcc
      901 gtccgcgacg gcaagaccga cgaggtctac aacacgatgc tttcggcgaa gatgaaagag
      961 gtcttcacgc tcgaggactg gaacgagtat gtgtcccgcg tctacaaagg caagatcggt
     1021 caggaactgc gcaccgccta cgccaccatc gtgaaggaga aatcgaccgg cgaattcatc
     1081 gaggcgaccg gacgcacctc caacatgggc cgtatgggca atgtcatgaa tcaggacgag
     1141 acgctcatct tccaggtgca tgtggtcaac gagaacggcc agttcaaggt cgagcagtct
     1201 gaggacatgt ccgctctgca ggaagagaag gacgcccaga aacggctcga cgaactgctc
     1261 aacaactatc agggactcgt caaactcgag gatgtgatgg tcgagccgct accgcagcga
     1321 cccggctggg cgctcgtctc gggcaccatt ctcaacgcga gttccgatct cgatatggtc
     1381 cgcgtcggcg tcaagatccg cctcaaggac tcggcgggcg acatcatcta cgctcagaag
     1441 ttcttcccgg tgctcgatgt ccgctacgaa gggctccgca cgagcgtcct gcccggcgac
     1501 tcgaaactgt tccagacccc gatcaaggac atcccgcccg agtgggataa gaaccaaccg
     1561 cccgacttcc agttctacct gatcgacggc accgttatat cggaagagga tctcaagaag
     1621 gagatcgtcg accgcgacaa gatgcgcaag ttggtcgagg agacccgcaa gggggacgaa
     1681 gaggcgcgcc gccagatgaa cgaacagtgg gacaaggaaa agcgtctcaa ggcgaagatc
     1741 aaggcgatgc aggagcgcag caaggcgaaa gaggaaaaga aataattttc cttggatgct
     1801 ggaataatca gtgatactgg gtggttatgc ggtcgtatcg gctattaagt tgacaggggt
     1861 atcgctttgt ggtacagaat gacgctgctc gcggttcttt tatttttgag tgctgaagcg
     1921 ttcgccgatc gcgatctgta catcatctgg ctcccttcga acgccgtcaa gaaagcggag
     1981 aaggtctaca ccatcccgct tgacctcgac ggcgcggtga agaaggtgaa acagcggatg
     2041 gcgcaggacc cgctgatccg ttccgatgtc ctcctcaacg aggacgggtt ccggatccat
     2101 gcgttctaca acctgcgaca gaccggcgct tggcgcaaga tgtacctcat agaggaggac
     2161 agccgcgtca ccgcgcggtt cttctgagat ccgggatcat ctaatggcag gattacggat
     2221 tctgactccg tcagtctagg ttcgactcct agtcccggat ccattttttt atagacgacc
     2281 ccatcgtcta gtggtcagga caccggcctc tcacgtcggg aacaccggtt caaatccggt
     2341 tggggtcgcc agttttctcc ccgttcaggg gatggttacc gtaacgtcat caaggtagac
     2401 agatccgccg gtcgccgtgc cgcccgtttg ttgatagacg cgggtgccga tcgtgagata
     2461 ggcggatcct gcaggcgagg taggcgaggc cgttatggtc tgccaatcga cctgatcggt
     2521 ggtgtagatc ccggggtaga cggtggatcc tatctgtgct tttgatgcgt cgtagaagga
     2581 ataatagagc gtaccgcgac cgttcgcgtc gttgtcgtaa taatggaagg ttatgttgta
     2641 ggcggtgttc tcggcgatcg ccacgaaatc ggaaacgaaa tcggtgttgc tgttggtcgt
     2701 gctgttcctc gtcagtttca ccgacgccgc gctcccgccg tgcgtgatgg tcgtctcctt
     2761 cgcgacgccg acgccgctat cggcggtcca tgaatcgggc gtcgtcccgt tggtccacag
     2821 ttcgaagtca ccgttgagca ccgtcagccc gtcggcctcg cagtagtggg tggcgaggtt
     2881 gcaggtcggc gtcggttcgg cgcagtcgcc atcaccgtcg caacgcccgg tcttgagcac
     2941 acaggtatcg acctgacatt cctcccagtc ctcgcaggtg tcgctaccgc aggtcaccgg
     3001 ggtcttcacc atatcgtcgg ctccgcgcgg caggattttt gagttgtcga acgcgaagaa
     3061 tagcacgccg gtgatcgcat cataggcgac cccgacctcg ggcatcgtat agaagtagag
     3121 ttggctgtcg acgcgcagat cgccggtgac gaggaactca ccgtaaccga gcgcgttgtc
     3181 gagcaccgtg accccttcga ccgtcacgag gaccgcgttg tggtcgtcgg cgtactgtcc
     3241 gcctgtcgcg atgtcggcgg gatcgacgat gaccggcgcc ggcagatcgt tgcctgaact
     3301 ttcgaccgtg atcgaggata tcgcctccaa ctcgagcagg ccgaagtagt tggtcaacgt
     3361 cccgacgacc gtcacgatgt cgccgaccgc cggtgcggtg agcgaactgt agacatagat
     3421 gccgctgaag gcgtagccga tcaccgggtc atggtccgcc tcggggacct gcaggaagaa
     3481 actggcgccg ttgagcgcgg tcaccacgcc acgcgtctcg accttgttac ccggcagaac
     3541 ggtgccgtcc ttgagatcgt agatggggat gatgccgcac gaaccgtaca tcgggttagg
     3601 actctcggga caggggtcgc agacatttcc ggcaccgtcg ccgtcgtcat cgagttgatc
     3661 gatgttggcg tcgtacgggc agttgtcgga ggcgttcggt atcccgtcac cgtccttgtc
     3721 gtcgggatcc accgtcggat cggcaccgag cggcgtcggg tcgcagaggt cgccgacgcc
     3781 gtcattgtcg gcatcgccct gcaccccgtt gtcgaccggg cgtatcggat tgaaaagcat
     3841 ggggcagttg tcgctcccgt tcgcgatacc gtcgccgtca aggtcttcca tcgtcggtcc
     3901 gttgtacggt cgagcatcgt cactcacggt ccgcaacggc gtgcaggtcg gttcgcccga
     3961 cggctcgcca caggaaaaaa gagcgtacgc ggaagagttc gccgcagcga gttgtgcata
     4021 ggtcttgccg gtctcgcgct cgacgcagac cgacttcggg acggtgcata ccgtcaaggc
     4081 ctcgcatccg ctctgtccgc ccggcacgac cgccatgacc gccgcgtcgc cgtagagcgg
     4141 ttcgccgccg cgcaggacga gcgccacgcc atcgacattg gcatcgatga tcgcgaggta
     4201 cggatcgtcg tcgtacccgg caccgtcgaa tatcgcgagg tcggcgacct tgccgacctg
     4261 tagcgagccg gtgagcgcgg cgagccggag cgcggtggcg ttgttgatgg tcgccatgcg
     4321 ccacagatcg cgcgccgtga agaacccgcc gtaaaagacg acgttcaggc gatcggcgca
     4381 tttgagttcg cgcaacatgt tcatcgagcc ggacgcggtc cagtcggtcc ccatgccgat
     4441 gttgacgccg agcgtgcggt aaagcgtcac cggcgcggtg ttgccgtaga gtgcgatgtt
     4501 ggtgcgcggg gaccagatga gcgccgtccc gttctgggcg aggatgaggg catcctgcgc
     4561 cttgagaccg acgaggtgga tgaaggcgct gttggcctcg gtgaggtcgc ggccgcctcc
     4621 ggcggaactc gaaagacaga ggaattcgtt gcgcgcctcg gcgtctatcc cctccgagac
     4681 atggggcagg tagcagtcgt tgttgagcac cgtcgccggg tctatggagg ggtagccgca
     4741 accgctcgcg aggagcgtgc cgccgctgtc accgagcggg aatgtgttgt attgcacatc
     4801 ggcgaaggcg agcccttcgg tattgaagta gttctggtca aggttgcgga ggaacccggt
     4861 cgcgccgccc gaaccggcga tggcggtggt ccccgccatc acattgcgca attcccccca
     4921 ctgcttctgc tcgacggtcg cgctgcccgg gaccgggatc ttggtgtggt cgcgtatccc
     4981 caatcgccag tcgtggcggt ggtcgaaccg ttcggtcccc cagttcccgg gggcgttgta
     5041 ggtgtacccg agatgatcgt gcgcgttgat gagcgccggg gatatgaccc ctgcggcgca
     5101 ctcgacgacc gtcgcgccgg cgtaccccga ctcggccgag cagtcgcacc cgacgcaggc
     5161 gatgtcgccc gccgcgccga cgagcacctc gccgccacgg tatatcttat cgtggccgag
     5221 caccacgccg cgcaacagca ggtcgtcgga accggcggtc gccgcgcaca gtccctcggc
     5281 aggcgggatc agttcggcgc aaaagatctc ctcctcgccg ccggcatccg tgtcggggac
     5341 ctcggcgtct tgatcggact ggtccgactg atcggtcggg tcggactggt cggcgtcggt
     5401 cgataccgcg tcatcggtcg ccatgccgtc gtcggtcgcc gtcgcaacga ccatatcgtc
     5461 cagcgagcgc ggttcgagtt tcgcattgtt ccatgtgtag tgcatgatgc cggtaagcgc
     5521 ctcgtagagc gtcccgaccg gcgggaaggt gtaggtgaac agcgtgtcgt cgacccgcag
     5581 gtcccccgta acgaggaact cgccgttgcc aagacccgtg tcgagcaccg tcaccccctc
     5641 taccgacacg agga
//
LOCUS       DAOQUE010000143         5647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15745_length_5647_cov_4.090129, whole genome shotgun sequence.
ACCESSION   DAOQUE010000143 DAOQUE010000000
VERSION     DAOQUE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15745_length_5647_cov_4.090129"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1354
                     /locus_tag="PKH10_05705"
     CDS             74..1354
                     /locus_tag="PKH10_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNT27661.1"
                     /translation="MKKFKILVFPCGSEIGLEIHRSLRYSAHLDLYGGSSVDDHGRFV
                     FEKYIGGIPFIDSPDVISSLKETIVTYGIDAIYPTMDKVIWKLKAHEEELGCKVISSC
                     LKTTEMCLSKKKTYALLADKTKTPKIFGSLGDVEEYPIFIKPDIGYGTRGVFKAESRE
                     KAEGFLSDKKKEDHVLSEYLPSDEFTVDCFTNRKRELMFVGPRKRWRIVNGISVNTTI
                     EKDEDNEFVNMARLINDTLEFRGAWFFQVKKDKKGHMTLLEVASRLGGSSSLYRGRGI
                     NFALLSVYDAFNVDVEILANQYGIEMDRALDNKYKLDLEYSTIYVDYDDCLLIDNKVN
                     HELLSFLFKALNQKKKIFLLSKHEGDLTASLKTNRLEGIFDRVIQIGKNERKVDHLDP
                     SGAIFIDDSFAERKAVSRQYGIPVFSPDMIETLI"
     gene            1371..2435
                     /locus_tag="PKH10_05710"
     CDS             1371..2435
                     /locus_tag="PKH10_05710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNT27662.1"
                     /translation="MRILHTYCLNYNLGDYALGIGVKNLLRQHLDVDLIGETNLQGTL
                     FNSYYIEEVVNKRYDLLVIGGGGVIHGAHWPNGWFWLIKEENIRKIKIPFIVYGVGYN
                     YFKDEGGIPEIGRSHLKETIKRAAYFSLRNDGSVDRFKEQMGIEVPEVPDPGFHMNLN
                     KTYSCPEKASYVIVQLANDKPAHRFGSDENKARFIADMRAVVSRIAKDHKVIFAPHVL
                     DDVSLSQEIAGGIENAFVWDFSKYAFDNVAESIAYYQDAHYVLAMRGHGQILPMAFNV
                     PVISIENHPKHSGLMRRLGLENYNVSLREDGFRDKLTETIRHLETNKDSYVKKLLSKN
                     AEMLKDSQDAFEVIRTRILS"
     gene            2444..3454
                     /locus_tag="PKH10_05715"
     CDS             2444..3454
                     /locus_tag="PKH10_05715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT27663.1"
                     /translation="MIKVSINCLAYNHEKYIKDALDGFLSQKTDFEYEVLIHDDASTD
                     KTADIIREYEKRYPHIIKPIYQTENQYSKGIRPTFAYNLPRAQGRYIALCEGDDYWTD
                     PLKLQKQVDLLEKDPAVAVCAHNYDIRTMRPDGSSFMERPLCRIAARSYSTAEIIAKG
                     MFVKTASLVFRRALIEEMLADIEYHLGFRVGDYPLLFFLSTKGPLHVLEESMSVYRKN
                     LSSAWNPLDARKKFLVSIETYERLLSRYPDFRKELERRISVCRMFLLKSLLDTGDLSQ
                     FLETLRKDIAASSSPSYACICHLAYFLIVRYLVDAISFGIRKVRRIGAILFSLFLPRG
                     SA"
     gene            3451..4278
                     /locus_tag="PKH10_05720"
     CDS             3451..4278
                     /locus_tag="PKH10_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT27664.1"
                     /translation="MIELLLSSLRTPERALNRFLERNDDPLRGLALLVVEQIPDRQDA
                     PLSEEGSSSAPQTGRLRVLSYRERGLSLSRNRALDNASGDICLLSDDDVRYRPDLADI
                     LSRAFEENPEADIITFQATDPDGRPLKNYPRRATRLNILSASRVSSIEIAFRHSSVLK
                     SDLRFDTLFGLGAKYPIGEEYVFVTDALKRGLTVIHLPIPILTHPLESSGRKHDAKTT
                     FARGAMLARVFGWAALPLDLVFALKKLPLYRKDRPFMSYLSSLWSGSWDYLKKRTSF"
     gene            4361..5434
                     /locus_tag="PKH10_05725"
     CDS             4361..5434
                     /locus_tag="PKH10_05725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT27665.1"
                     /translation="MKVSILIPTYQESRYIGRCLESLVANDYDFAGSDSEILVIDGHS
                     TDGTRDIVTELVREHPFIRLIDNPDTVQVVGLNVGLRIAAGDIIVRCDAHSEYPHNYI
                     SELVKWHEKGLAANIGGCWITMPGDDGLRAQAIASVMRHPLGVGFTYRTVQKGRERFV
                     DTVPFGSWRRETLEKFGHFDEEFTRAQDLEHNVRLRQQGQKILMLPWLRIRYFARETF
                     DKAASMFYQYGYWKNKVNKKYGTLCSLRQLIPPLFVLGFFSLLIATIVSFVISSPFAS
                     AIASFLAFYSGVYLTAVSISSLFIIARHKDPCRRSFRFFTLLVCAFVATHFSYGIGYL
                     HGFIEIVLFNRKQLDSRLKDNTR"
     gene            5538..>5647
                     /locus_tag="PKH10_05730"
     CDS             5538..>5647
                     /locus_tag="PKH10_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27666.1"
                     /translation="MKIAVVGSGYVGLVSGACLADFGLTVICMDIDEKKIA"
BASE COUNT         1382 a         1494 c         1437 g         1334 t
ORIGIN      
        1 gatattcccc gccatcaccg atgaggaaat cgagaataca tgccggataa taaggccata
       61 gtggattgat gtcatgaaaa aattcaagat tctggttttt ccctgcggat cggaaatagg
      121 tctagagata caccggtctt tgagatattc tgctcacctt gacctctacg gcggcagtag
      181 tgtagatgat catgggaggt tcgtgtttga aaaatatatc ggcgggatcc ccttcataga
      241 ttcccccgat gtcatttctt ccttgaaaga aacgattgtc acctacggta tcgatgcgat
      301 ctaccccacc atggacaaag tgatttggaa attaaaagca catgaggaag aactgggatg
      361 taaggtgatc tcttcttgtc tgaagaccac ggagatgtgt ctttccaaga aaaagaccta
      421 tgctttgctt gcggacaaaa caaaaacgcc aaagattttc ggatcattgg gcgatgttga
      481 agaatacccg atattcatta aacccgatat cggatatggg acaagagggg tgttcaaggc
      541 cgagtcaagg gaaaaagcgg aagggttcct gtcggacaag aaaaaagaag atcacgtgct
      601 ctcggaatat cttccttccg atgagttcac ggtggactgt ttcaccaaca ggaaaagaga
      661 attgatgttc gtcggaccga ggaagagatg gcgtatcgtc aatgggataa gcgttaatac
      721 cacgatcgag aaggacgagg ataacgaatt cgttaacatg gcgcggttga tcaacgacac
      781 gctggaattc agaggagcat ggttctttca agtgaaaaaa gacaagaaag gacatatgac
      841 actccttgaa gttgcatcac ggctcggcgg ctcctcttct ctgtatcggg gcagagggat
      901 caactttgcg ctcttgagcg tgtacgatgc attcaatgtt gatgtggaga tactagccaa
      961 ccagtacgga atagaaatgg atcgggcctt ggacaacaag tacaaactcg atctagagta
     1021 ttcaacgata tatgtcgact acgacgattg cctgttgatc gacaataagg tcaatcatga
     1081 attgctctct tttcttttta aggcgttgaa ccagaaaaaa aagatattct tgctctcgaa
     1141 gcacgaaggc gacctgacgg catcgttgaa aacaaatcgc ttggaaggca tttttgaccg
     1201 ggtcatccag atcggaaaga acgaaaggaa ggtggatcac cttgacccat cgggggccat
     1261 tttcatagat gattcttttg ccgaaagaaa ggcggtgtcg agacagtatg gcattccggt
     1321 cttttcgcct gacatgatag aaacactgat ctgaacattg aggcgttgtc atgaggatcc
     1381 tgcacaccta ctgtcttaat tataatcttg gcgattatgc cctcgggatc ggcgtcaaga
     1441 atcttttgag acagcatctt gatgttgacc tgatcggtga gaccaatctg caggggacgc
     1501 tgttcaacag ttattacatc gaagaggtgg tcaacaagag atatgatctg ctggtgatcg
     1561 gtggcggcgg ggtgatccac ggcgcgcatt ggcctaacgg atggttctgg ctgatcaaag
     1621 aggagaacat acgcaagatc aagatccctt tcatcgtata cggtgtcgga tacaactact
     1681 ttaaggacga agggggcata ccggagatag gcagatcgca tctaaaagaa accatcaaaa
     1741 gggccgcgta tttctcactt cgcaacgatg gcagtgttga tagattcaaa gaacaaatgg
     1801 gaatagaggt gccggaagta ccggatcccg ggttccatat gaacctgaac aaaacctatt
     1861 catgtcccga aaaagcgtcg tatgttatcg ttcagcttgc caacgataaa cccgctcacc
     1921 gtttcgggag cgatgagaac aaggcgagat tcattgccga tatgagggcg gtggtctccc
     1981 ggatcgcaaa ggatcataag gtgatattcg ccccccatgt tttggatgac gtttctcttt
     2041 cgcaagagat agccggcggc atagagaacg cctttgtgtg ggatttctcg aaatacgctt
     2101 ttgataatgt cgccgagtcc atagcatact atcaggatgc gcactatgtg ttggctatgc
     2161 gagggcatgg ccagatactc cccatggcat tcaacgtgcc ggtcatatcg atagagaatc
     2221 acccgaaaca ttcgggactt atgagacgat tggggctgga aaactacaat gtgtcgttgc
     2281 gggaggatgg cttcagggac aaacttacgg agacgatacg gcatcttgaa acgaacaagg
     2341 atagttatgt caaaaagtta ttgtcaaaga acgcagagat gctgaaagat agccaagatg
     2401 cgtttgaggt cataagaacc cgcatattaa gttaaggaag ggtatgatca aggtcagcat
     2461 taattgcctt gcctacaatc acgaaaagta catcaaagat gcgttggatg gttttttgtc
     2521 acaaaagacc gacttcgaat acgaggtgtt gatccacgat gatgcctcga ccgataagac
     2581 cgccgacatc atccgcgaat acgagaagag gtacccgcat atcatcaagc cgatctatca
     2641 gacggagaac caatactcaa agggtattcg ccccactttt gcctacaatc ttcctcgagc
     2701 acaaggcaga tacattgccc tttgcgaagg ggacgactac tggaccgacc cgctgaaact
     2761 gcagaaacag gtcgatctcc tggagaagga tcccgccgtg gccgtctgtg cgcacaacta
     2821 cgatatacgc accatgcggc ccgacggttc gtccttcatg gagcgaccat tgtgccgcat
     2881 cgccgcccgc tcgtattcga cagcggagat catagcgaaa gggatgttcg tcaagaccgc
     2941 ctcgctggtc tttcgccgcg cccttatcga ggagatgctc gccgacatag agtaccacct
     3001 cggttttcgc gtcggcgatt atcccctact ctttttcctt tcgacaaaag ggccgctcca
     3061 tgttctcgag gaaagcatgt cggtctatcg caagaacctc tcaagcgcat ggaaccccct
     3121 tgatgccagg aagaaattcc ttgtttcgat agagacgtac gaaagactgc tttcccgtta
     3181 tcccgacttt aggaaggaat tggagcgccg gatatcggtc tgtcgcatgt tcctgctgaa
     3241 gagccttctc gacaccgggg atctctccca attccttgag accctcagaa aggatatcgc
     3301 cgcttcctcc tccccttcct acgcgtgcat ctgccatctg gcatacttcc tgatcgtacg
     3361 gtatctcgtg gacgcgatct catttggcat aagaaaggtc cggcgtatcg gagccatcct
     3421 cttttcactt tttctgccca gagggtccgc atgatagaac tgctcctctc ttctcttcgc
     3481 acaccggaac gggcattgaa caggttcctt gagcggaatg acgatcctct tcgaggactc
     3541 gccttgctgg tcgtggagca gataccggac cggcaggacg ccccattgtc ggaagaagga
     3601 tcttcctccg cgccccagac cggtaggctc cgggtgctga gctatcgtga aaggggtctg
     3661 tccctgtcgc ggaaccgcgc tttggacaat gcttcggggg acatctgtct cctgagcgac
     3721 gacgatgtgc gctatcgtcc cgatctcgcc gacatactgt cccgcgcatt cgaggagaac
     3781 cccgaagcgg acatcataac ctttcaggcg accgaccccg atggcaggcc acttaagaac
     3841 tacccccgcc gtgcgacccg cctgaacatc ctgagcgcct cccgtgtctc ctccatcgag
     3901 atcgcgttcc gacactcatc ggtgctcaaa tcggatctgc ggttcgacac ccttttcggc
     3961 ctcggcgcga aatatccgat cggcgaagaa tatgtctttg tgaccgatgc gttaaaacgc
     4021 gggctcaccg tcatccatct tcccataccg atcctcaccc accccctcga aagttcgggg
     4081 agaaaacatg acgcgaagac cacctttgct cgcggcgcga tgcttgctcg ggtttttggc
     4141 tgggccgctc ttccgctcga tcttgttttt gcgttaaaaa aactgcctct ctacaggaaa
     4201 gaccgtccct ttatgtcata tctctcctcc ctgtggtccg gatcttggga ctacctgaaa
     4261 aaaagaacat ctttttgaag attccctccc taaacttgtc ttttgctccc gaaagcgtat
     4321 agtgggagca ccttaagtgg tgagaggcgt cgcaagacac atgaaagtat caatacttat
     4381 accaacttat caggagtcga ggtatatcgg tcgctgcctt gagagtctcg ttgccaacga
     4441 ctacgacttc gccgggtccg attccgagat cctggtgata gacggtcatt cgaccgacgg
     4501 cactcgcgac atcgtcaccg aacttgtccg cgaacacccc ttcatccgcc tgatagataa
     4561 tcccgacacc gtacaggtcg tggggctcaa tgtggggctc aggatagcgg ccggcgacat
     4621 catcgtccgg tgcgacgccc attcggaata tccccacaac tatatctcgg aactggtgaa
     4681 gtggcacgag aaaggccttg cggccaacat cggcggctgt tggataacga tgccgggcga
     4741 cgacggcctg cgggcccaag ccatcgcatc ggtcatgcgc cacccgctcg gtgtcggctt
     4801 cacctaccgc accgtccaga aagggcgcga acgcttcgtg gacaccgtgc cgttcggctc
     4861 gtggcggcgt gagacgctcg agaagttcgg tcatttcgac gaagagttca cccgtgcgca
     4921 ggacctcgag cataatgtgc gtctccgcca gcagggccag aagatactga tgctcccgtg
     4981 gctccgcatc cgctatttcg cccgcgagac cttcgataag gcggccagta tgttctacca
     5041 gtacggctac tggaagaaca aggtcaacaa aaaatacggc accctctgca gcctgcggca
     5101 acttatcccg cccctgttcg tgctcgggtt cttctcgctc ctcatcgcca ccatcgtatc
     5161 cttcgtgatc tcctccccgt tcgcgtcggc gatcgcctcc ttccttgcct tctactccgg
     5221 cgtttatctt accgccgtct cgatatcctc tcttttcatc atcgcccgtc acaaagaccc
     5281 ctgtcgccgc tccttccgct ttttcacctt gctcgtctgc gccttcgttg ccacccattt
     5341 cagttacggc atcggttatc tgcacgggtt catcgagata gtgcttttca atcgcaagca
     5401 gcttgattcc cgactgaagg acaacacccg ctgacggtcc gcacgccatc cgaaatatcc
     5461 cctgaatcct tgacctcgcc ggtcaaatcg ctacagtgct tcgcggcata ggattctctt
     5521 gtgtaaggag gaacaacatg aagatcgcgg tcgtcggctc cggctatgtc gggctcgttt
     5581 ccggcgcctg tctcgccgat ttcggcctta cggtcatctg catggacatc gacgagaaga
     5641 agatagc
//
LOCUS       DAOQUE010000144         5625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15820_length_5625_cov_4.963375, whole genome shotgun sequence.
ACCESSION   DAOQUE010000144 DAOQUE010000000
VERSION     DAOQUE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15820_length_5625_cov_4.963375"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKH10_05735"
     CDS             complement(<1..192)
                     /locus_tag="PKH10_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27667.1"
                     /translation="MKRAARIATALFFAIFVWVAVGVFSALFVYGGRLSFAIYDSEFF
                     SVTPEKVEITGIARLTREEI"
     gene            complement(189..1091)
                     /locus_tag="PKH10_05740"
     CDS             complement(189..1091)
                     /locus_tag="PKH10_05740"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNT27668.1"
                     /translation="MILTRYTRIAVVMGGLSPEREVSLDSGTRVKDALVQGGYTAVTP
                     VDMGRDIDVVLRDLRPEVVFNALHGAYGEDGRLPGLLDIIGIPYTGSGVTASAVAMDK
                     ILTKRVARTLGIPLAADTVVTAVSGLAAPMPYPFVFKDPLNGSSHGVFIVRDEAGWRD
                     ALAESTGKRMLVEEYVKGREITVAVLGDAVLGDVEIVPAKEFYDYEAKYKDENTRYIP
                     DPDYDPATREAMRRAALDIHRTLGCRGATRTDFIVTPERHIMLEINTHPGMTGHSLVP
                     MAAAKRGVDYLGLIERLLTEALAV"
     gene            complement(1104..1943)
                     /locus_tag="PKH10_05745"
     CDS             complement(1104..1943)
                     /locus_tag="PKH10_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNT27669.1"
                     /translation="MKLKKRAALTRFLTIRPRDAVCDLYFPESIDDLVRFTVLERDYL
                     LLGGGSNIIAGAVRKPVITFALLEGSSVTEDIDRRQVAVSMPAWTRTGALVDYLVANG
                     LSGLEFMAGIPGTVGGAIVGNAAPKGHSWEGVVRKVIYVKEGSVLSAEPRFAYRRTLD
                     MPEPPYILLAAEIVLTKDTVAKVRGRVKGFLKQRLAVPYPSAGSLFKNPERSSAGELL
                     ETAGCKGLAVGDAALYEKHANIVVNRGDAAYADFVALRDEAIARVKKMHGVQLEPEVK
                     FWQ"
     gene            complement(1943..3292)
                     /locus_tag="PKH10_05750"
     CDS             complement(1943..3292)
                     /locus_tag="PKH10_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNT27670.1"
                     /translation="MHIHFMGAGGSGVSGLMLIARSMGHLVSGCDQHRTGYLDMLERQ
                     GVPVTTGHDQAHLAGVDLLVCSSAVPNGHMEVMAARMKGIPVIRRGDLLAKILEGRAV
                     IGIAGAHGKTSTAWLTGRLLRRFVVPASLYSGGRSQGTSSVVAGDPWVVELDESDGSI
                     FNIRPQTLVITNLELEHAEYYGTEQEMLRRFRSHLALIGHRRFVIGRGYPVSDRLFEE
                     FGGLTFPTRAEIAAKREVRGGRDCRFLCDGGNWTLDFHGTPSDLGTAREPAYVIQNRA
                     AALLAAYTHLRDIGRRPEPVPLEFWDTVPPVERRFERKGSFRGLALIDDYAHHPSEVQ
                     ALMDRAGVEFGRYLMVFQPHRHTRFDRFMGEFAKVLESAPALLILPVYGAGEPRGTKG
                     SRELYEILKQKGMRSVHYADTLGEAAVTISAHNFDWVRAIITVGAGDVNDIFGMLEE"
     gene            complement(3292..4392)
                     /gene="murG"
                     /locus_tag="PKH10_05755"
     CDS             complement(3292..4392)
                     /gene="murG"
                     /locus_tag="PKH10_05755"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNT27671.1"
                     /translation="MNGNKGMTILLAGGKTGGHLFPGIAVAEELRARGVPVAFVGTRG
                     GLEEQELPARGFELDIIRAGGLKGKGLFDTLRNLAVLPFALLRSWLIIRRRRAGAVVS
                     LGGFAAGPVALAARLSGRRVFVMEQNTVPGITNRIVGRFADRIFLSFPDIRGVFPPTR
                     AVLTGNPVRREVIEGAPRELGDGRQVLAVFGGSQGAHSLNEMLMHLARAYPGDLGNYF
                     VFHQTGAQDLDAVRRVYAESGIAAEAVPFVRDVGAVYRAAAVIVCRAGATTIAELAAL
                     RRPAVYVPFPFAADNHQYHNARFMTERGGGALVEDAGTVEERGERLRAALKELAERRE
                     EWSRRMAAELPGERADRRIADEILAPTMAGGA"
     gene            complement(4400..5482)
                     /locus_tag="PKH10_05760"
     CDS             complement(4400..5482)
                     /locus_tag="PKH10_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HNT27672.1"
                     /translation="MSQAVRFYFGLFFLLVLFGMVMVFNIKVFVVDRPDAQVFSIFRS
                     QAVNLVIAAIAFIAAYLVNVDIIRGSIKYLLLFIILLLLATFVIGTEVNGAKRWINLH
                     FMMVQPSEFAKLVVIFYLAEVICNKRDRLGLWKEVIYPFSLVSAIVLVIAVEDLGTAL
                     IIFSTSLVLMLMGGMRVKYFAVLLSFALLVVTVLILMKPYRLERLRVWQDPWKYKQAQ
                     GYQQVQSEVALGRGGMTGVGFGNSQRKLRYLPEASTDFIFAIIGEEFGFVGSTALIGA
                     FLAFFLLGGYFVLSRYDPFSFYLTAGIVLMLGIQAAINLGVVTSTLPNKGVPLPFISY
                     GGSSLIVSSLMVGFLLNVVVSEGRGY"
BASE COUNT         1056 a         1903 c         1746 g          920 t
ORIGIN      
        1 tatctcttcg cgggtaaggc gcgctatgcc ggttatctcg accttttcgg gcgtcaccga
       61 gaagaattcg ctgtcgtaga tcgcgaaaga caggcggccg ccgtacacga agagggccga
      121 gaacaccccg acggcgaccc acacgaagat cgcgaagaaa agcgccgtcg ctatgcgtgc
      181 cgcccgtttc ataccgcaag cgcctccgtg agcaagcgtt cgatgagccc cagataatca
      241 acgccccgtt tcgccgctgc catcgggacg agcgaatgac ccgtcatccc ggggtgggta
      301 ttgatctcga gcatgatgtg acgttccggc gtcacgatga agtcggtgcg ggtcgccccg
      361 cggcacccga gcgtccggtg gatgtcgagc gccgcgcgcc gcatcgcctc ccgcgtcgcc
      421 ggatcgtaat cggggtcggg gatgtaacgg gtattctcgt ccttgtattt cgcctcgtag
      481 tcatagaatt ccttcgccgg gacgatctcc acatcaccga gcaccgcgtc gccaaggacc
      541 gcgaccgtta tctcgcgccc cttcacatac tcctcgacga gcatccgctt gcccgtgctt
      601 tcggcaagag cgtcgcgcca acccgcctcg tcgcgaacga tgaagacgcc gtgactcgac
      661 ccgttcagcg ggtccttgaa cacgaagggg tacggcatcg gcgccgcgag tcccgacacg
      721 gccgtcacga ccgtatcggc ggcgagcggt atgccgagcg tgcgcgccac ccgtttggtc
      781 agtatcttgt ccatcgcgac cgccgacgcg gtgacgcctg acccggtgta ggggatgccg
      841 atgatgtcga gcagtccggg aagacggccg tcctcgccat aggcaccgtg gagcgcgttg
      901 aacaccacct cggggcggag atcccgcagg accacatcga tgtcgcgccc catgtcgacc
      961 ggcgttacgg cggtgtagcc gccctgcacc agcgcatcct tcacccgcgt tcccgaatcg
     1021 agactgacct cgcgttccgg cgacaggccg cccatcacga ccgcgatgcg ggtgtacctc
     1081 gtcagtatca tggtctcact ccctcactgc cagaacttca cttccggctc cagttggacg
     1141 ccgtgcatct tcttgacccg cgcgatcgcc tcatcgcgca atgcgacgaa gtcggcatag
     1201 gcggcgtcac cccggttcac cacgatgttc gcgtgcttct cgtacagcgc cgcgtcgccg
     1261 accgcgagcc ccttgcaccc cgccgtttcg agcagttctc ccgccgaaga cctttccggg
     1321 ttcttgaaga gcgagccggc cgacgggtac ggtaccgcga gacgctgttt caggaaaccc
     1381 ttcacccgcc cccgcacctt cgcgacggtg tccttcgtca gcacgatttc ggcagcgagc
     1441 aggatgtagg gcggttccgg catgtcgagg gtgcgacgat aggcgaaacg cggctcagcg
     1501 gagagtacgc tcccctcctt gacatagatg accttccgga cgacgccttc ccacgaatgt
     1561 cccttcggcg cggcgttacc gacgatcgcg ccgccgacgg tgcccgggat gcccgccatg
     1621 aattcgagcc ccgacaggcc gttcgcgacg aggtagtcga caagcgcccc ggtgcgggtc
     1681 cacgccggca tcgagacggc gacctgccgc cggtcgatgt cctcggtgac gctactccct
     1741 tcgaggagcg cgaaggtgat gaccggcttt cgcaccgcgc ccgcgatgat gttcgagccg
     1801 ccgccgagca ggagatagtc ccgttcgagc accgtgaaac ggacgaggtc gtctatcgat
     1861 tcggggaagt agaggtcgca caccgcatcc cgcggccgta tggtgaggaa ccgggtgagt
     1921 gcggcgcgct ttttcagctt catcactcct ccagcatccc gaagatatcg ttcacatcac
     1981 ccgcgccgac cgtgatgatg gcgcggaccc agtcgaaatt gtgcgccgat atggtcaccg
     2041 ccgcctcgcc gagcgtatca gcgtaatgga ccgaccgcat ccccttctgc ttcagtatct
     2101 cgtacagttc acgcgacccc ttcgtgccgc gcggctcgcc ggcgccgtac accggcagga
     2161 tgagcagggc gggcgccgat tccagcacct tcgcaaattc gcccatgaag cgatcgaacc
     2221 gggtgtgacg gtgcggctgg aagaccatca ggtaccgccc gaactccaca ccggcacggt
     2281 ccatgagcgc ctgcacctcg gatgggtgat gtgcatagtc atcgatgagc gcgagaccgc
     2341 ggaacgaacc tttcctctcg aagcggcgct ccaccggcgg cacggtgtcc cagaactcga
     2401 gcggcaccgg ctcgggccgt cgcccgatat cgcgcaggtg agtgtaggca gcgagcaggg
     2461 ctgcggcgcg gttctgtatc acataggccg gttcgcgcgc ggtaccaaga tcgctcgggg
     2521 taccgtggaa atcgagcgtc cagttgcccc cgtcgcatag gaaccggcag tcgcgtccgc
     2581 cgcgcacctc ccgtttcgcc gcgatctccg cgcgggtcgg gaaggtgagc ccgccgaact
     2641 cctcgaacag gcggtcgctg accggatagc cgcgcccgat gacgaagcga cgatggccga
     2701 tgagggcgag gtgactgcgg aaccggcgga gcatctcctg ctcggtcccg taatattcgg
     2761 catgctcaag ttcgaggttg gtgatgacga gcgtctgggg ccgtatgttg aatatcgaac
     2821 cgtcgctctc gtcgagttcg accacccacg gatcgcccgc gacgacgctg ctcgtcccct
     2881 gcgaccgccc gccgctgtag agcgacgcgg gaacgacgaa gcggcgcagg agtcggccgg
     2941 tcagccacgc ggtgctcgtc ttaccgtgcg cgccggcgat gccgatgacc gcgcgacctt
     3001 cgagtatctt cgcgagaaga tcgccgcgcc ggatgaccgg tatccccttc atgcgggccg
     3061 ccatgacctc catatgcccg ttgggcaccg ccgaggaaca gaccagcaga tcgacacccg
     3121 ccagatgggc ctgatcgtgg ccggtcgtca ccggtacccc ctgccgttcc aacatatcga
     3181 gatagccggt ccggtgctgg tcgcacccgc tcaccagatg ccccatcgat cgcgcaatga
     3241 gcatcagccc gctcaccccg ctcccgcccg ctcccatgaa gtggatgtgc atcacgcgcc
     3301 tcccgccatc gtcggcgcga gtatctcgtc ggcgatgcga cgatcggccc gttcgcccgg
     3361 cagttccgcc gccatgcgcc gggaccattc ctcgcggcgt tcggcgagtt ccttcagcgc
     3421 cgcgcgcagg cgctcgcccc gctcctcgac cgtcccggcg tcctccacca acgcgccgcc
     3481 gccccgttcg gtcatgaaac gggcgttgtg gtactggtga ttgtcggcgg cgaaggggaa
     3541 cgggacatag accgccggtc tccggagcgc cgcgagctcc gctatcgtcg tggcgccggc
     3601 acggcacacg atgaccgccg ccgcccggta caccgcaccg acgtcgcgca cgaacgggac
     3661 cgcctcggcg gcgatgcccg actcggcgta gacgcgacgc accgcatcga ggtcctgcgc
     3721 gccggtctgg tggaacacga aatagttccc gaggtcgccg gggtatgcgc gggcgaggtg
     3781 catcagcatc tcgttgagcg agtgcgcccc ctgcgagccg ccgaacacgg cgagcacctg
     3841 ccggccgtca cccagttcgc gcggcgcccc ttcgatgacc tcgcgccgca ccggattacc
     3901 ggtcaacacg gcacgcgtcg gggggaaaac cccccggata tcgggaaaac tgaggaatat
     3961 gcggtcggcg aaacggccga cgatacggtt ggtgatgccg gggacggtgt tctgctccat
     4021 cacgaagacc cggcggccgc tcaatcgcgc cgcgagcgcg accggcccgg ccgcgaaacc
     4081 gccgagcgac accaccgcgc cggcgcggcg tctgcggatg atgagccacg accgcagaag
     4141 cgcgaaaggc aggaccgcga ggttccggag cgtatcgaag agacccttgc ccttgagccc
     4201 gccggcacgg atgatgtcga gttcgaaccc gcgcgcgggc aattcctgtt cctccaatcc
     4261 gccgcgggtg ccgacgaagg cgaccgggac gccgcgcgcc cgcaactcct cggcaacggc
     4321 gataccgggg aacaggtgcc cccccgtctt gccgcccgcc agtaatatcg tcatgccctt
     4381 gttcccgttc atagcgtact cagtaccccc gcccctcgct gaccaccaca ttgagcagga
     4441 accccaccat cagcgacgag acgatcaggg aactcccgcc gtaactgatg aaggggagcg
     4501 gcacaccctt gttgggaagc gtcgaagtga ccactccgag gttgatggcc gcctgtatcc
     4561 cgagcatgag cacgatgccc gcagtgagat agaacgaaaa gggatcgtag cgcgacagga
     4621 cgaaatagcc gccgagcagg aaaaaggcaa ggaaggcgcc gatgagcgcg gtcgacccga
     4681 caaagccgaa ctcctcgccg atgatcgcga agatgaagtc ggtggacgcc tcgggaaggt
     4741 aacgcaattt tcgctggctg ttgccgaatc cgacgcccgt catgccaccg cggccgagcg
     4801 cgacctccga ctgcacctgc tgataaccct gcgcctgttt gtatttccac gggtcctgcc
     4861 agaccctgag ccgctcgagc cggtatggtt tcatgaggat gagcaccgtg acgaccagaa
     4921 gcgcgaacga gagaaggacc gcgaagtatt tgacccgcat accgcccatg agcatcagga
     4981 cgagcgaggt cgagaagatg atgagcgccg taccgaggtc ctcgaccgcg atgacgagca
     5041 cgatggccga cacgagactg aagggataga tgacctcctt ccagagcccc agccggtccc
     5101 gtttgttgca gatgacctcc gcaagataga atatcacgac gagtttggcg aactcggagg
     5161 gttgcaccat catgaagtgc agatttatcc accgtttcgc gccgttcacc tcggtgccga
     5221 tgacgaaggt ggcgaggagc aggaggatga tgaacaggag cagatacttt atcgaaccgc
     5281 ggatgatatc gacattcacg agataggcgg cgatgaaggc gatcgccgcg atgaccagat
     5341 tgaccgcctg cgaccggaat atcgagaaca cctgcgcgtc gggccggtcg acgacgaaca
     5401 ccttgatgtt gaacaccatc accatgccga agagcaccag caggaaaaaa agtccgaagt
     5461 agaagcggac cgcctgcgac atcgcctaca tcccctcttc gtccttcagt atcttcacca
     5521 ctcggacgaa atccttgccg cgctccttgt agttctcgta ccaatcgaaa ctggcgcagg
     5581 cgggggagag cagcaccgtg ccgcttcccg ccgcgagccg gaagg
//
LOCUS       DAOQUE010000145         5613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15863_length_5613_cov_4.189277, whole genome shotgun sequence.
ACCESSION   DAOQUE010000145 DAOQUE010000000
VERSION     DAOQUE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15863_length_5613_cov_4.189277"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PKH10_05765"
     CDS             complement(<1..691)
                     /locus_tag="PKH10_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27673.1"
                     /translation="MDSEGNDLTPPDLTQLFAWGELKYGAETKTTEAVWMGDDDATLR
                     FTGLPYGVSMSLTLTMRAADTDGDDSDIVGLDDVPEVRTDNIVYYCTSQAFSLTRGTV
                     TDVNNACVMQEGPAAGEGNKPGAPVMTLSYTDDDDKETTIDTATEFKTPKKIIAIEFT
                     APNDNFDTVVVANDTGFALGRKEYPKPEKANGAYRIESHDLDDGLPVTTDGSRTIALK
                     LRNALGFESLAA"
     gene            complement(872..2860)
                     /locus_tag="PKH10_05770"
     CDS             complement(872..2860)
                     /locus_tag="PKH10_05770"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNT27674.1"
                     /translation="MIGRFIEFGKHLIEVSRYDPEIDLDRWREQLFTVIYPLTWVVGL
                     AACVGTLHSAFAEDRYVYPLLAVSAYLLVMGIMGLRRLGVRTRMTIALLVFFSSVFYQ
                     SSPLIGYLFLFSFPPLVATLVSFRAALSAIMANILLFILLSYHFASMDLDLSRFGVGS
                     IDQWILYGIVFLFLDIIITACFGLLTKGLAQIIVLERDSRRMIAEKERRLEEEILRSA
                     RMRESLSETERKAAFYISHDRLTTLPNREAFMQRVGVEIMRARNRGQVFSIASVGIDK
                     FKHINSIYGPSGGDAVLVAFTERLRTLVRDNDVIGRTGDDRFMLLFSDLARVEDVLAV
                     LAKLWETIERPFQVIGQEIRISASIGLCFFPHDAKSPEDLLKNSETAMFAIKEAGGGA
                     HRFFDAQLNTDMTERINLERMLRTAVTQDQFVPFFQPKVDRYGYLLGMEALIRWHAPT
                     GLIMPDRFIPLAEQNGIILDIGERVLYRACRQNRDWQEQGLVPKKVSVNLSPFEFRHP
                     DLVRSIESTIHKSGLDPMWLELEITESGIARNEQDAIVKLAQLHDMGISLSIDDFGTG
                     YSALSRLRDYPIDTLKIDKSFIDNIPQDKRSVTIVISLIKLAHNLGFKVVAEGVERPE
                     QLEFLVLQGCDQFQGYFFSKPLSAEEFEKKLQPQVIMS"
     gene            complement(2857..4230)
                     /gene="purB"
                     /locus_tag="PKH10_05775"
     CDS             complement(2857..4230)
                     /gene="purB"
                     /locus_tag="PKH10_05775"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNT27675.1"
                     /translation="MQELRALSVLDGRYAKQVEELRDIFSEYGLIRRRVLVETLWLEF
                     LLGDLGLAPATAGDIEAIRQVAAGCNLEAAKLVKEYEKKTNHDVKAVEYFVKEQLAAA
                     GLARLGEWTHFACTSEDINNAAYALMLRDGRDALLGRIEELRDALADRAAQWRAVPMM
                     ARTHGQPASPTTVGKELVVFASRLDREIAKLRDWRPEAKLNGATGNFNAHAAALPGID
                     WIAASERFLRERLDVTPLLYTTQINPYHHIAELLQALVRVSRTVTDIDRDVWDYISFG
                     YFRQRTVAGEVGSSTMPHKVNPIDFENSEGNCGVAVALMEHLSAKLLVSRFQRDLSDS
                     TTLRDLGALFGHLLIALKSTLKGLGKLELDETTLRADLDANPELLAEAIQTVMRRYGE
                     ERPYERLKELTRGERVSREALAAFIDTLAKVPEKERKRLRALTAADYTGLATALTDRY
                     LAGRGKP"
     gene            4347..4422
                     /locus_tag="PKH10_05780"
     tRNA            4347..4422
                     /locus_tag="PKH10_05780"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4380..4382,aa:Val,seq:tac)
     gene            4439..4515
                     /locus_tag="PKH10_05785"
     tRNA            4439..4515
                     /locus_tag="PKH10_05785"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4473..4475,aa:Asp,seq:gtc)
     gene            4596..>5613
                     /locus_tag="PKH10_05790"
     CDS             4596..>5613
                     /locus_tag="PKH10_05790"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNT27676.1"
                     /translation="MHFVSSCITDIGRRRSNNEDWLLEWPDRGLYVVADGMGGGKHGE
                     VASHLAVEAVEQFLTEHFPVVQSFRLTRSEENRRQVMDLVADAARHANTAVYEEAKKS
                     RSAGKMGTTLTGLLLVDTEGFLFHVGDSRLYLIRNAMAIQLSADHTMQRDYQETYGAV
                     RGIDAGLSNTLTRAVGLASSVEVDVRAIDVRVNDKLLLCTDGVHRYISPGRIEEIAHH
                     LSVPGVDAAEERRFLDSEVKRLADEVHEAGAEDNLSIILVAARAAEPETAVAERFLDL
                     FSVARALPLFARFEDAQIERVVHAAEVRTLKKYDIIQFPLNPEGELLVMLEGQVSVLR
                     RSRQI"
BASE COUNT         1021 a         1642 c         1850 g         1100 t
ORIGIN      
        1 tcgcggcgag cgattcaaag ccgagggcgt tgcggagttt cagcgcgatg gtgcgagaac
       61 cgtcggtggt gacgggaagc ccgtcgtcaa ggtcgtggct ttcaatgcgg taagcgccgt
      121 tcgccttctc cggtttggga tattctttcc ggcccagcgc gaacccggtg tcgttggcga
      181 cgaccacggt gtcgaagttg tcgttcggtg cggtgaattc tatcgcgatg atctttttcg
      241 gggtcttgaa ttcggtggcg gtatcgatgg tggtctcttt atcatcgtcg tcggtgtaag
      301 aaagggtcat gaccggggcg cccggcttgt tcccttcgcc ggctgccggt ccttcctgca
      361 tgacgcaggc gttgttgaca tcggtcaccg tgccgcgggt cagggagaat gcctgcgagg
      421 tgcagtaata gacgatattg tcggtgcgca cctcgggaac atcgtcaagc ccgacgatgt
      481 cggaatcgtc gccgtcggtg tcggcggcac gcatcgtgag cgtcagggac atcgagacgc
      541 cgtaggggag cccggtgaag cggagcgtcg cgtcgtcgtc gcccatccag accgcctcgg
      601 tggtcttggt ctcggccccg tacttcagtt cgccccacgc gaacagttgg gttaggtcgg
      661 gtggcgtgag gtcgttcccc tcgctgtcca cccacgagta gtggagccgg atcccccccc
      721 ggttcgttgc tctcgcaggc gacgaggaac agcgcgacca acacgaccga taaccacgcg
      781 atacggttca tgatgccacc ccgcagacga atattttctt tgaagcattg tatcactttt
      841 tcgtcgggaa gcaaaaaaac agaagggcga attacgacat gatcacctgc ggctggagtt
      901 tcttctcgaa ctcctccgcg gagagcggtt tgctgaaaaa atagccttgg aactggtcgc
      961 atccctgcag gacgaggaac tccaactgct cgggtcgctc gaccccttcg gcgaccacct
     1021 tgaacccgag gttgtgcgcc agtttaatga gcgagatgac gatggtcacc gaccgtttgt
     1081 cctgcgggat gttgtcgatg aaactcttgt ctatcttgag tgtgtcgatg gggtaatccc
     1141 tgaggcgcga gagcgccgaa tacccggtgc cgaaatcgtc tatcgagagc gagatgccca
     1201 tgtcgtgcag ttgcgcgagt ttcacgatgg cgtcctgttc gttgcgcgcg atgccgctct
     1261 cggttatctc gagttcgagc cacatcgggt cgagcccgct tttatgtatc gtgctttcga
     1321 tggagcggac gaggtcgggg tgacggaact cgaacggcga gagattgacc gacacctttt
     1381 tgggaacgag cccctgttcc tgccaatcgc ggttctggcg gcaggcgcgg tacagcaccc
     1441 gttcgccgat gtcgaggatg atgccgttct gctcggcgag cggtatgaag cggtcgggca
     1501 tgatgagtcc ggtcggggcg tgccagcgga tgagcgcctc catgccgagc aggtagccgt
     1561 agcggtcaac cttgggttgg aagaagggga cgaactggtc ctgtgtgacg gcggtacgga
     1621 gcatccgttc gagattgatg cgttcggtca tgtcggtgtt gagttgcgcg tcgaagaacc
     1681 ggtgggcgcc gccgcccgcc tccttgatag cgaacatcgc ggtctcggaa ttcttcagaa
     1741 gatcctcggg tgatttggcg tcgtggggga agaaacagag cccgatgctc gccgatatgc
     1801 ggatctcctg cccgatgacc tggaacgggc gctcgatggt ctcccagagt ttggcgagca
     1861 cggcgagcac atcctcgacc cgcgcgagat cggagaacag gagcatgaag cggtcgtcgc
     1921 cggtgcggcc gatgacatcg ttgtcgcgca cgagcgtccg gagccgttcg gtgaaggcga
     1981 cgagcaccgc gtcgccgccg gacggaccgt atatgctgtt gatgtgcttg aacttgtcta
     2041 tgccgaccga ggcgatcgaa aagacctgtc cgcggttgcg cgcccgcatg atctcgacgc
     2101 cgacccgctg catgaacgcc tcgcggttag gcagggtggt gagccggtcg tgcgagatgt
     2161 aaaaggcggc cttgcgttcg gtctcggaga gcgattcccg catccgggcg ctccgcagta
     2221 tctcctcctc gagccgccgc tccttttcgg cgatcatccg gcgcgaatcg cgctcgagga
     2281 cgatgatctg cgcaagcccc ttggtcaaca ggccgaagca ggcggtgatg atgatgtcga
     2341 ggaacaggaa cacgatgccg tacagtatcc actggtcgat cgacccgacg ccgaaccggc
     2401 tcaggtcgag gtccatcgag gcgaaatggt aagagaggag tatgaacagc aggatgttcg
     2461 ccatgatcgc cgacagcgcg gcgcggaaac tcacgagcgt cgcgacgagt ggcgggaacg
     2521 agaaaaggaa cagatagccg atcagaggcg aactttggta aaagacgctg gagaagaaga
     2581 caagcaacgc gatcgtcatg cgggtacgga cgccgagccg tcgcaacccc atgatcccca
     2641 tgacgagcag ataggccgat accgcgagga gcggatagac gtaacgatct tcggcgaagg
     2701 cgctgtgcag ggtgccgacg caggcggcga gtcccacgac ccaggtgagc ggatagatga
     2761 cggtgaacag ttgttcccgc caacggtcga ggtctatctc ggggtcgtaa cggcttactt
     2821 cgatgaggtg tttcccgaac tcgatgaacc gtccgatcat ggtttccccc gcccggcgag
     2881 gtaccggtcg gtgagcgccg tcgcgaggcc ggtgtaatcg gcggcggtca gggcccgcag
     2941 ccgcttccgc tctttttccg ggaccttggc gagcgtatcg atgaaggccg cgagcgcctc
     3001 gcgggacacc cgctcaccgc gtgtcagttc cttcagccgt tcgtagggcc gttcctcgcc
     3061 ataccgccgc atcaccgtct gtatcgcttc ggcgagcagt tcagggttcg cgtcgaggtc
     3121 ggcgcgcagg gtcgtctcgt ccagttccag tttgccgagt cctttcagcg tacttttgag
     3181 ggcgatgagg aggtggccga acagcgcgcc gagatcgcgg agcgtcgtcg agtcggaaag
     3241 gtcgcgttgg aaccggctga cgagcagttt cgccgaaagg tgctccatca gcgcgacggc
     3301 gacgccgcag ttcccttcgc tgttctcgaa atctatcggg ttgaccttgt gcggcatcgt
     3361 cgaggagccg acctcgccgg cgacggtccg ctggcggaag tagccgaatg aaatgtagtc
     3421 ccagacatcg cggtcgatgt cggtcaccgt ccgcgaaacg cggacgagtg cctgcaggag
     3481 ttcggcgatg tggtgatagg gatttatctg ggtcgtgtag aggagcgggg tcacatcgag
     3541 tctttcgcgc aggaaccgtt ccgacgcggc gatccagtcg atcccgggta gagcggcggc
     3601 atgggcgttg aagttgccgg tcgcgccgtt cagtttcgcc tcggggcgcc agtcgcgcag
     3661 tttcgctatc tcgcggtcga gccgggaggc gaagacgacc agttccttgc cgacggtggt
     3721 cgggctcgcc ggctggccat gggtgcgagc catcataggg acggcgcgcc actgcgccgc
     3781 gcggtcggcc agcgcgtcgc gcaactcttc gatccggccg agcagcgcgt cgcgaccgtc
     3841 gcggagcatc agggcgtagg cggcgttgtt gatatcctcc gaggtgcagg cgaaatgggt
     3901 ccactcgccg agccgcgcga gtcccgccgc ggcgagttgt tctttgacga aatattccac
     3961 cgccttcaca tcgtggttgg tcttcttctc atattctttg acgagtttcg ccgcctcgag
     4021 gttgcatccc gccgcgacct gccggatcgc ctcgatgtcg ccggcggtcg ccggggcgag
     4081 tcccagatcg ccgagcagga actcgagcca gagcgtctcg acgagcacgc gccgccgtat
     4141 caggccgtat tccgaaaaga tgtcgcgaag ttcctcgacc tgtttcgcat agcgaccgtc
     4201 gaggaccgaa agggcgcgaa gttcctgcat gccatacctc ggtcagatgg gcgcatcata
     4261 acataaaaag aggaaaagtc cacccgcgcg cgataaaaaa taagttgaca aaacggcgtt
     4321 ctccgataac aatggcgccg ttggtcgggt gcttagctca gctggtagag catcggtttt
     4381 acacgccggt ggtcataggt tcgagtcctg tagcacccac catttggttc tttgaggcgc
     4441 ggtcgtagtt aagttggtta taacgccggc ctgtcacgtc ggaggccgcc ggttcaagtc
     4501 cggtcgaccg cgccattttt ctttttcccc gcaagccaaa agttgacttt gccatccccg
     4561 tacgatattt ttccgggcac gaaaagggag cgctcatgca cttcgtctcc tcatgcatca
     4621 ccgacatagg ccgccggcgc tccaacaacg aggactggct gctcgagtgg cccgaccggg
     4681 ggctctatgt ggtcgccgac ggcatgggcg gcggcaagca cggcgaggtg gcctctcatc
     4741 tggcggtcga ggcggtcgaa cagttcctca ccgaacactt cccggtcgtg cagtcgttcc
     4801 gcctgacccg gagcgaggag aaccgccggc aggtcatgga tctcgttgcc gatgcggccc
     4861 gccacgccaa cacggcggtg tacgaggagg cgaaaaagag ccggagcgcc ggcaagatgg
     4921 ggaccacgct gacaggcctg cttcttgtcg atacggaggg gttcctgttc catgtgggcg
     4981 actcgcgact ttacctcatc cgcaacgcga tggcgataca actctcggcc gaccatacca
     5041 tgcaacgcga ctatcaggag acctacggcg ccgtgcgcgg gatagacgcc ggtctttcga
     5101 acacgctgac ccgcgccgta gggctcgcat cgtcggtcga ggtcgatgtc cgcgccatcg
     5161 atgtccgcgt caacgacaaa ctactgctgt gcaccgacgg ggtgcatcgc tatatctcgc
     5221 ccggtcgtat cgaggagatc gcgcatcacc tctcggtgcc gggggtcgac gccgccgagg
     5281 agcggcggtt cctcgacagc gaggtgaagc ggctcgccga cgaggtccac gaggcggggg
     5341 ccgaggacaa cctctcgatc attctcgtcg cggcacgcgc ggccgagccg gagacggcgg
     5401 tcgcggagcg gttcctcgat ctgttcagcg tcgcgcgggc gttaccgctt ttcgcccggt
     5461 tcgaggacgc ccagatagag cgggtggtcc acgccgccga ggtgcgcacc ctcaagaagt
     5521 acgacatcat ccagttcccg ctcaaccccg agggggaact gctcgtcatg cttgaaggtc
     5581 aggtgtcggt gctacgccga tcgcgccaga tag
//
LOCUS       DAOQUE010000146         5608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15887_length_5608_cov_6.781740, whole genome shotgun sequence.
ACCESSION   DAOQUE010000146 DAOQUE010000000
VERSION     DAOQUE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15887_length_5608_cov_6.781740"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            535..>5608
                     /locus_tag="PKH10_05795"
     CDS             535..>5608
                     /locus_tag="PKH10_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008483355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27677.1"
                     /translation="MKVSSHVMLFLFATLFSFYVYASQTNYQYQVTGNEISVEFYGNQ
                     ALFSNGAGHETPGYPDMRHFNVYFLLPDDYVPSNISVSLDNVESENILGEWEVPPVKP
                     ETMYVDGQPVEYMPDKTNVVHGKNMIAYSRNNYIFGSFLGSYVITSANRYNILRVTIY
                     PFDYNPFTKELKKLTSGTLTVSLDTNNIIDIPHDLSLTKRKLLSELSDMVVNSNHIVV
                     RTQSFIRPLLSFIPTIQAINGNTNDRYYIVTTEDVVQNSLYLGKFIASKKNRGMTVYL
                     VTEEKTRKDGIIIESSGWGQVGLVGDSAANALRSYLKAGYPPKYLDIDYLLLIGNPHP
                     GTCVRRAGMLTNPEDDKNPDNSPADTLTCLNEGQDAGTLPMKRINMFSNSEWEGYCSN
                     WNWAMGRCDGVWYPANDVRRSTPSDLYYAELSNVLVGHTIKDPWDLDNDGLVGENELK
                     IDLRNDNELINLDIKPDIMVGRIPIYTRNRFPPAISGPDYESLDRVFSEIIKYENESK
                     HSVSQWRNNMFLPMDQLSEKHPGWYSAEITMDSLPATIDFKYTIYDEQYFNEATGLNT
                     KEYDNNFNDCDNMGTSYKTCAMPVVTGNDLCDITKIGVSHYIPCLLAAGDIGAQLQEC
                     DGISWVNNGTCFDNGSENKNLDASIPILDFNCICTIDWTAYRSYLGNPIQDSTASDPL
                     HGNIDQECVDAPCELTYDHYDFGLVFSNAHGGRDSTGGIITSTHVTNHPKDRRSIWFT
                     QSCQNGWPEDPRNLGTRLLYNSSVVVVAGTRNMQYDSNCFDEIKDAFDCFSTDDPTVE
                     SGDTFAYFFFKSLTDESLSDRITAGQAYRKMHNVIVGDNHRRNRIQFNMYGDPSLGLD
                     TYSKKGNDSDGDGVIDEFDPCPHNAAVTRWGQDLDGDGVSDVYDSDDDGVADICDNCL
                     GLPNPYVKADKEIIFGARINANTIGQTTIYNGEYYWQPDHDLDGVGDVCDTQSDYIEP
                     KSFHGSYKDNPSTKIRTQNEYAVFYVKAESFQSDKDLRATTRKCWVNNDRKNRWGDPG
                     LCTTEKEIYDIENCKHTFGYSHGSDPNAYFDFKETWREISWSKNVGYNAETVLSSHWY
                     QYNYPAWDESKPMQGERANDTGYLHIKGNIEGKKPVSMELYWNWRDDMVYDDETYAKS
                     KVETPRNISGFPWAEDSAVAWELNDFYVVLSSGVKGPGAIYQSNNVINPEFFRIDGTT
                     GQPVPRFARTARLQYDPIPLTYYNFSIRDIPLKEDKYIIDQYHYFEKRLSELVVDKRE
                     AGGALIQKWKVSTGGNIDITYKRKSSSMLAMVPSIDGNDYGIEKRPLVEGGATYHLVR
                     SLSSSFGDWQDLGEITVPNNETNFEIEAYYHDGQDLYVTTKYLTGNVASVTVKELYRV
                     PGPIILICEGGFLPVHTPIKLADVSPDMEHITIHALGEKKYLLGSDSTGMKLYELTET
                     ELIAISTPTGIGVRDFYNAAVVDGALYLAGGGNPSTEYKDVWKYSSSQGWQQLASGLN
                     ADLYKVFIAGTDGILYLTSQIPINGNIVDQITMTYDGGSVSVGKITLADFSSTAHEYC
                     LYDDGALLKGGIGSYETCQPFIRPWYKSFSIGTTVYSVAGKGDRLYVGTNSTIRVYDI
                     SDPNAMVLKSTFTTNRRVYDLEVAEGDIMYAATSGGIYKFNTANPDALSQLSFFSTPY
                     NYQYRIQLYNDKLYVGDDNGINI"
BASE COUNT         1697 a         1191 c         1310 g         1410 t
ORIGIN      
        1 ccgagcgagt acgcgcaacg atgaaacggg aacgcggggc ctacggcctc aacgggagcg
       61 ggagcggacc ctggcttgtc ccgctatcgt gtccggcgac ttgtccggca tagctttcag
      121 cgaagccgga agttagacga agacggaagc gaagcgacgg cggaaagggt ggggagaagg
      181 gggcaagtga attttttgtc aagatgtacc ttttttcttg acttctcttc aagagagatt
      241 tctcatacgg tcaatatgac atatcgaaac cagcgggtat ctgcgcatgg ggatggtgcg
      301 gaaagactcc ttgccgagac gggacggcaa aggtctcggg cggaaaaata aaatagttct
      361 gtacaatatc ggagagttac gatcaagatt aaaaaaagca gaatcctttt cccgcacact
      421 cgtctcttat ggatgtcaag gggaagaggt agatcgcgtg cggagaagtt tagcgggaac
      481 catgcataga cacgggggca aaaccatcaa caacggagat agagggagga gaagatgaaa
      541 gtttcatcac acgtcatgct ttttttgttt gcgaccctgt tctcttttta tgtctatgcg
      601 tcacagacga actatcagta tcaagttacg ggcaacgaga tttctgtaga attttatggc
      661 aatcaagcac ttttcagtaa tggcgcagga cacgaaactc caggatatcc cgatatgcgt
      721 catttcaatg tttacttctt gttgcctgac gattatgtgc cctcaaacat ttcggtatcg
      781 ttggacaatg ttgagagtga aaatatacta ggcgaatggg aagtgcctcc cgtcaaaccc
      841 gaaacaatgt atgttgacgg gcaaccggtg gaatatatgc ccgataaaac aaatgtcgtt
      901 catggaaaaa acatgattgc atattcaaga aacaactaca tctttggctc ttttcttggt
      961 agttatgtta tcacctcggc aaacagatac aatattttaa gagtcacgat ttatcctttc
     1021 gattataatc catttacaaa agaattgaag aaattaacat ctgggacact gacggtttcc
     1081 cttgatacga acaatataat tgacattccc cacgacctct ctttaaccaa gagaaaactt
     1141 ttatcggaat tgtcggatat ggtcgtaaat agcaatcaca ttgttgtaag gactcagagc
     1201 tttattaggc cattgctgtc tttcattccg actatccaag ccataaatgg caataccaac
     1261 gacagatact acattgtgac gacagaagac gtggtccaga acagcctata ccttggtaaa
     1321 ttcattgctt caaagaaaaa ccggggcatg acggtctatc ttgtaacaga agaaaaaact
     1381 cgaaaagatg gaatcattat cgagagtagt ggatggggac aagtaggtct agtaggtgac
     1441 agcgcggcca acgccttaag atcatatcta aaagcgggct accccccaaa atatttagat
     1501 atagattacc ttctcttaat aggaaatcct catccgggca cctgtgttcg aagagcagga
     1561 atgctcacaa atccagaaga tgataagaac cctgataata gccccgcgga caccctgact
     1621 tgtttaaatg aggggcaaga cgccggcacc ttgcctatga aaagaataaa catgttttcc
     1681 aatagtgaat gggaaggata ttgcagtaac tggaattggg ctatgggaag atgcgatggc
     1741 gtttggtatc ccgctaacga tgttagaaga tcgacgccgt ctgatttgta ttatgctgaa
     1801 ttgtcaaatg ttcttgtcgg gcacaccata aaagatccat gggatctgga taatgacggc
     1861 cttgtgggag aaaacgagct caagatagac ttaagaaatg acaacgaatt aattaatctc
     1921 gatattaaac ctgacataat ggtagggaga atcccaatat atacgagaaa tcgcttccct
     1981 cccgcaatct ctggaccgga ttacgaaagt cttgatcgtg tgttttcaga aattattaaa
     2041 tatgagaatg aatcgaaaca tagtgtctcg cagtggcgta ataacatgtt tttgccgatg
     2101 gatcagttaa gcgaaaaaca cccgggatgg tactcagcag aaataaccat ggacagtctt
     2161 cccgccacga ttgattttaa atatacgata tatgatgagc aatatttcaa cgaggcaacc
     2221 gggcttaata ctaaggagta tgacaacaac tttaatgatt gcgataacat gggcacatca
     2281 tataaaacat gcgccatgcc tgttgttaca ggaaatgatc tttgtgatat cacgaaaatt
     2341 ggtgtcagcc attatattcc atgtcttttg gccgcaggtg atatcggcgc tcaattgcaa
     2401 gaatgtgatg gcataagttg ggttaataat gggacatgtt tcgacaacgg aagcgagaac
     2461 aaaaatcttg acgcaagcat acccattctt gattttaact gtatatgtac tatagattgg
     2521 actgcatata gaagttatct ggggaatccg atacaagaca gcactgcttc ggaccctttg
     2581 catggaaata tagaccaaga atgtgtagac gcgccctgtg agttgaccta tgatcattat
     2641 gatttcggcc ttgttttttc taacgctcat ggtggacgag actctactgg cggtataata
     2701 acaagcaccc atgttactaa tcatcccaag gacaggagat caatatggtt tactcagtca
     2761 tgccaaaatg gatggcccga agatccgcga aatctgggca caaggctatt gtacaactca
     2821 tctgttgttg ttgttgctgg tactcgtaat atgcaatatg attctaattg ctttgatgaa
     2881 ataaaggatg ctttcgactg tttttctacc gatgacccaa ccgtcgaaag cggcgatact
     2941 tttgcctatt tcttttttaa atccttaacc gacgagtctc tttctgatcg cataacagct
     3001 ggtcaggcat atcggaagat gcataatgtt attgttggtg ataatcatcg aagaaaccga
     3061 attcaattca atatgtacgg agacccgtct ttgggattgg acacctattc gaaaaaaggg
     3121 aatgattccg atggcgacgg ggtcattgat gaatttgatc catgtcccca taatgctgcg
     3181 gtgacccgtt ggggacaaga ccttgatggt gatggggttt ctgatgtcta cgattccgac
     3241 gatgatgggg tggccgatat ttgcgacaac tgcctcggtt tgccgaaccc ctatgttaaa
     3301 gcagataaag agataatctt cggagcaaga ataaacgcaa ataccatagg gcaaactacc
     3361 atttataacg gtgaatatta ctggcaaccg gatcacgacc tcgatggtgt cggcgatgtt
     3421 tgcgatactc aaagtgatta tattgaacca aaatcatttc atggctcata caaggacaat
     3481 ccatcaacga aaatcagaac acaaaacgaa tacgcggtat tttatgtaaa agcggaaagt
     3541 tttcagagcg ataaggacct aagagccacc acaagaaagt gctgggtaaa taacgataga
     3601 aaaaacagat ggggagaccc gggattatgt acgacagaaa aggaaattta tgatatagaa
     3661 aactgcaaac acacctttgg ttatagccac gggagcgatc ccaatgcata ttttgacttt
     3721 aaagaaactt ggcgagaaat atcgtggtca aaaaatgttg gttataacgc ggaaaccgtc
     3781 ttgtcatcac attggtatca atacaattat cccgcatggg atgaatcaaa acccatgcaa
     3841 ggagaaagag cgaacgacac aggatatctt catataaaag ggaatatcga agggaaaaaa
     3901 cctgtatcta tggagttgta ttggaactgg cgagacgaca tggtgtatga tgatgagact
     3961 tatgcgaaat caaaggttga aactcccaga aacatatccg gattcccgtg ggcagaagat
     4021 tctgcggtag catgggaact taacgatttc tatgtagtcc tctcttcggg agtaaaagga
     4081 ccgggagcaa tatatcagtc taacaatgtg attaaccccg aattcttcag gatcgatggc
     4141 acaacaggac aacccgttcc tcgatttgct cgaacagcca gattgcaata tgatcctatt
     4201 cccttgactt actataactt cagcatccga gatatccccc taaaggaaga caaatacatt
     4261 attgaccaat atcactactt cgaaaaacgt ctttcagaac tcgtggtgga taaacgagag
     4321 gcgggaggag cgctgataca gaaatggaaa gtatcaacag gcggcaacat tgatattacc
     4381 tataaaagaa aatcttcatc aatgctagcc atggtcccaa gcattgacgg caatgattac
     4441 ggcatcgaga aaagaccctt ggtggaaggt ggcgctactt atcacttagt gaggagcctg
     4501 tcctctagtt ttggcgattg gcaagacttg ggtgaaataa cggttcccaa caatgagacc
     4561 aatttcgaaa tagaggcata ttatcacgac gggcaagacc tctatgtcac gacaaagtac
     4621 ctaacgggca atgtcgcgag cgttacagta aaggaactgt atagggtgcc gggacctatc
     4681 atattgattt gtgaaggggg ctttcttccc gtccatacgc ctatcaaact tgccgatgta
     4741 tcgccggaca tggaacacat aaccatacac gcccttggcg agaagaaata tctcctagga
     4801 agcgattcga caggcatgaa gttgtatgaa cttaccgaaa cagaacttat tgccatctct
     4861 acgcccacag gtatcggcgt tcgcgatttc tacaatgcgg ctgtggttga cggtgcactg
     4921 tatcttgcag gagggggcaa tccttccaca gagtataagg atgtgtggaa atattcctcc
     4981 tctcaaggat ggcaacaatt ggcctcagga ctgaatgccg atctttataa agtatttatc
     5041 gctgggactg acggcatatt gtatcttacc agccagattc cgataaatgg aaatatcgtg
     5101 gatcaaatca cgatgactta tgatggtggc tcggtttccg tagggaaaat cacgcttgcc
     5161 gatttttctt caacagccca cgagtactgt ctctacgatg atggcgccct tctcaagggt
     5221 ggtattggtt catatgagac ctgccaaccc ttcatccgcc catggtacaa gtccttctcc
     5281 atcggcacga cagtctacag cgtcgccggg aaaggcgacc ggctctatgt cggtaccaac
     5341 agcacgatcc gcgtctatga catcagcgat ccgaacgcga tggtcctcaa gagcactttc
     5401 accaccaacc ggcgcgttta cgacctcgaa gtggccgagg gcgacatcat gtacgcggcg
     5461 accagcggcg gcatctacaa gttcaatacc gccaatcccg atgcgctgag tcagttgtcg
     5521 ttcttcagca cgccgtacaa ctaccagtac cgcatccaac tctacaatga caaactctat
     5581 gtcggcgacg acaacggcat caacatcc
//
LOCUS       DAOQUE010000147         5581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_15999_length_5581_cov_3.651104, whole genome shotgun sequence.
ACCESSION   DAOQUE010000147 DAOQUE010000000
VERSION     DAOQUE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15999_length_5581_cov_3.651104"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..990)
                     /gene="miaA"
                     /locus_tag="PKH10_05800"
     CDS             complement(124..990)
                     /gene="miaA"
                     /locus_tag="PKH10_05800"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNT27678.1"
                     /translation="MIVLLGPTASGKSAYAVTLAKEIGGEVIGVDAVQIYRDLVIGSA
                     APTAEERGPVPHHLIGLLDLSEDINAGRYIELARPVIEEVVARGKTPILVGGTNFYVD
                     AFLTGLSPVPELDEEKKDSFAERCAPVATATLYAELRAADPRWAVAISSPNDRQRILR
                     GLEVYHLTGTPLSVWNEKPREKGWGGQYRKIALSVEREVLNERIVARTKKMLAGGLLD
                     EVAAIHAKGFTPANCRVLGSIGYREAFDHLDGRIASKEELEELIVIHTRQLAKRQMTW
                     LKSDPEIAWIAP"
     gene            complement(987..2711)
                     /gene="mutL"
                     /locus_tag="PKH10_05805"
     CDS             complement(987..2711)
                     /gene="mutL"
                     /locus_tag="PKH10_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNT27679.1"
                     /translation="MGIVKRLPEQLIDQIAAGEVIERPASVVKELVENALDAGATDII
                     VEVARGGREMIRVADDGSGMGQEDLFMALERHATSKLSDAQGLSRIATMGFRGEALPS
                     IASVSVFSLASALAHGDGFRVAARGGVMTESGPVSRPKGTEATVGRLFFNLPARRKFL
                     KSEEREFAHIKELIQRLAVTHPSIAFRLIHNERTALSFAAATSVADRAAEVWKAGRDE
                     TTLVEETAGHTAGTLLLGSPYRSFAAPATVTVNRRIVSDRRVNAVIYRVIRESVGGDH
                     RPVYALSISLPPEEVDVNVHPAKSEVRFRDESAVFALIEALFRKGLAALRTGERSPEL
                     FPGAPTSPAHAALPRIAPSARPTGTELFSPPLPLTGVADTTPASFSPAIRITGYRVLG
                     TLFDTYIAVEMGDALYLIDQHASHERIVYTRLREAMEKKSGLTQLTLLAEPVHLSPVE
                     MAVWREHREMFADIGLTLEELDDETLVIRGVPALSLDPSWPSLVQDLLADIREQGFSS
                     AWDEKFLSIAADRACKRAVRAGRSLAPEEIERLIADINASEVLTCPHGRPFFFVVSKR
                     DIERGIGR"
     gene            complement(2713..4011)
                     /locus_tag="PKH10_05810"
     CDS             complement(2713..4011)
                     /locus_tag="PKH10_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27680.1"
                     /translation="MNGPGDTDAKRTAIAIVSFLAVIFALVSVFLWLHGEKRDYHLYL
                     LGDDLRTTPRLLARIVSRDGYLVPDARIELDGRPTPGMIFDATHPFRTVTLTIGGTAY
                     EYEADFGRMLEKSARRLPDAWFTEKEIQEAEAAVRRIATASKRLYVVPAAIRLFGEQP
                     NDITLFCLTDAGPCTDDRVIIGASSFALRNGIGTGTLTPSFQAEIMARFPDDTVVPLL
                     FPYPGKMFSLEKEDDRWVARTLVDMNNIHIDCWRGDRWEVTDVVTVPPTGRPLPSHYA
                     ACDRIQVSGDSHDPGGNFLVRAAGATPRDISDPYFRALLDDLDRHRPDAAAILLDRYP
                     AAFFRRLNIIFSSTEDEQAFEARKKDRLAIVWWLIAAVAAAGIALFSVSSYRRFTTVV
                     GDEEEPITLSRRQQKLIVIAASAALALFFVVLLSVLRNLA"
     gene            4279..4740
                     /locus_tag="PKH10_05815"
     CDS             4279..4740
                     /locus_tag="PKH10_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNT27681.1"
                     /translation="MSFESTRVKEILKDYAPEKGNLMAVLRELQKEGGNHLSDETLKE
                     VVRTIGIPLNRLYGFVSFYTMLSTAPKGRHIIRVCKDGPCNFAGGWATAELLEKELDI
                     EMGETTEDGRFTLEHTACLGTCTAAPAIMIDDETYANVTPEKLGEILKKYK"
     gene            4751..>5581
                     /locus_tag="PKH10_05820"
     CDS             4751..>5581
                     /locus_tag="PKH10_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNT27682.1"
                     /translation="MNYNAKILSADKDHVLLKNVGKIDPMSIDAYVKAGGYEGLKKAL
                     AMDPKKVIDEVSESGLRGRGGANFPTGRKWTFTAGAKADQKYIICNADEGEPGTVKDR
                     ILMEGDPHQMIEGIVIGAYAIGATKGFIYIRGEYRKSIETIEKAIADAEKKNFLGKGI
                     LGSKFDFELRVVEGGGAYVCGDETALIESIEGKRGEPRMKPPFPPVVGLWGKPTIVNN
                     VETLANVPHIIKNGAAWFLKKGPAGSPGTKIFMLVGDVNEPGVVETTFAQQPMRVFRG
                     V"
BASE COUNT         1041 a         1651 c         1914 g          975 t
ORIGIN      
        1 agagggaagg gaagatattc aagatataca tcaaatcgaa ccagaacatc gcggtgcgta
       61 agtcggagga ggaggggctc atccacttcg acttcttcgt gccgacgatg gagtgacggg
      121 tccttacggc gctatccacg ctatctcagg atcactcttg agccacgtca tctggcgttt
      181 ggcgagttga cgggtatgga tgacgatgag ttcttcgagt tcttccttcg acgcgatgcg
      241 gccgtcgagg tggtcgaacg cctcgcggta accgatggaa ccgagcacgc ggcagttggc
      301 cggggtgaag cccttggcgt ggatggcggc gacctcgtcg agcaggccgc ccgcgagcat
      361 ctttttcgtc cgggcgacga tccgctcgtt gagtacttcc cgttcgacgg agagcgctat
      421 cttcctatac tggccgcccc aacctttctc ccgcggtttt tcattccaca ccgagagcgg
      481 cgtgccggtc aggtgataca cttcaaggcc gcgcaggatc cgctggcggt cgttgggcga
      541 cgatatggcg acggcccagc gcggatcggc ggcgcgtagt tccgcgtaca gggtcgccgt
      601 cgccaccggg gcgcaccgtt cggcgaacga atcttttttc tcttcgtcaa gttcggggac
      661 cggcgagaga ccggtcagaa aggcgtcgac ataaaaattt gtcccgccga cgaggatggg
      721 ggttttcccc cgggcgacaa cctcttcgat gaccggacgg gcgagttcga tgtatctccc
      781 ggcgttgata tcttcggaaa ggtcgagcag gccgatgagg tggtgcggga caggtcctcg
      841 ttcttcagcg gtcggggcgg ccgagccgat gacgaggtcc cggtatatct gcaccgcgtc
      901 gacgccgatc acctcgccgc cgatctcctt cgcaagcgtg acggcatagg cgctcttgcc
      961 cgatgcggtc gggccgagga ggacgatcat ctgccgatcc cgcgctcgat gtcgcgtttt
     1021 gaaacgacga agaagaaggg cctgccgtgc gggcaggtga gcacctcgga ggcgttgatg
     1081 tcggcgatga gccgctcgat ctcctcgggg gcgagcgaac gaccggcgcg caccgcccgt
     1141 ttgcaggcgc ggtccgccgc gatgctcagg aatttctcgt cccatgccga cgagaagccc
     1201 tgttcgcgga tgtcggcgag caggtcctgc acgagggagg gccacgaggg atcgagcgac
     1261 agggccggga cgccgcgtat gacgagcgtc tcgtcgtcga gttcttcgag cgtgaggccg
     1321 atgtcggcga acatctcgcg atgctcgcgc cagaccgcca tctcgacggg ggagaggtgc
     1381 accggctcgg cgaggagcgt cagttgggtg agcccgcttt tcttctccat cgcctcccgc
     1441 agacgggtat agactatccg ttcgtggctc gcgtgctggt cgatgaggta gagcgcgtcg
     1501 cccatctcga cggcgatgta ggtgtcgaag agcgttccga ggacacggta ccccgtgatc
     1561 cgtatcgcgg gggagaacga agcgggggtg gtgtcggcca cgccggtcag cgggagcggc
     1621 ggcgagaaga gttcggtccc cgtggggcgg gccgacgggg cgatgcgtgg aagagcggcg
     1681 tgagcggggg atgtcggtgc gcccgggaag agttcgggcg accgttcgcc ggtacggagc
     1741 gccgcaagcc ccttgcggaa cagcgcttcg atgagcgcga agaccgccga ttcgtcgcgg
     1801 aaccgcacct cgctcttggc cgggtggaca ttcacatcga cctcttcggg ggggagcgat
     1861 atcgacagcg cgtagaccgg ccggtggtcg ccgccgaccg attcgcggat gacacggtag
     1921 atgacggcgt tgacgcgtcg gtcggatacg atgcggcggt tgaccgtcac cgtcgcgggg
     1981 gcggcgaagg agcggtaggg cgacccgagc aggagcgtcc cggcggtgtg gccggccgtc
     2041 tcctcgacga gcgtcgtctc gtcgcgtccc gccttccaga cctccgccgc gcggtcggcg
     2101 accgaggtcg cggcggcgaa ggagagcgcc gtgcgctcgt tgtgtatgag ccggaaggcg
     2161 atcgacggat gggtcaccgc gagccgctgg atgagttcct tgatgtgggc gaactcgcgt
     2221 tcctccgatt tgaggaactt gcggcgcgcc ggcaggttga aaaagagccg gccgacggtc
     2281 gcctcggtcc ctttcgggcg gctgaccggt ccgctttcgg tcatcacccc gccacgagcg
     2341 gcgacccgga agccgtcgcc gtgcgcgagc gccgaggcga gcgaaaagac gctgaccgag
     2401 gcgatggagg ggagcgcctc gccgcggaaa cccatcgtgg cgatgcgcga gagtccctgc
     2461 gcgtcggaga gtttgctcgt cgcgtggcgt tcgagcgcca tgaacaggtc ctcctgcccc
     2521 atgcccgagc cgtcgtcggc gacccgtatc atctcgcgac cgccgcgggc gacctcgacg
     2581 atgatgtcgg tcgcgccggc gtcgagcgcg ttctcgacga gttccttgac caccgatgca
     2641 gggcgttcga tgacctcgcc cgccgctatc tggtcgatga gttgttcggg gagtcgtttg
     2701 acgatgccca tgttacgcga ggttcctcag taccgatagt aataccacga aaaaaagcgc
     2761 gagcgcggcg ctcgccgcga tgacgatgag tttttgctgg cgccgcgaga gcgttatcgg
     2821 ctcctcctcg tcgccgacga ccgtcgtaaa gcggcggtac gaggagaccg aaaataacgc
     2881 gatgcccgcg gcggcgaccg cggcgatgag ccaccagacg atcgcgagcc gatccttctt
     2941 gcgcgcctcg aacgcctgct cgtcttcggt gctcgagaag atgatgttca gccgtcgaaa
     3001 gaaggcggct gggtaccgat cgaggaggat cgcggcggcg tcgggacggt ggcggtcgag
     3061 gtcgtccaga agcgcgcgaa agtaggggtc ggagatatcg cgcggcgtcg cgccggcggc
     3121 gcggacgagg aagttgccgc ccgggtcgtg cgagtcgccc gacacctgta tccggtcgca
     3181 ggcggcgtag tgggacggca ggggtctccc cgtcggcggg accgtgacca catcggtcac
     3241 ctcccagcgg tcgccgcgcc agcagtcgat atggatgttg ttcatgtcga cgagcgtgcg
     3301 ggcgacccag cggtcgtcct ccttttcgag cgagaacatc ttcccggggt aaggaaaaag
     3361 gagcgggacg acggtgtcat ccgggaaccg cgccatgatc tccgcttgga acgatggggt
     3421 cagcgtccct gtgccgatgc cgttacggag cgcgaacgac gacgcgccga tgatgacccg
     3481 gtcgtcggtg cagggacccg catcggtgag gcagaagagg gtgatgtcgt tgggctgttc
     3541 gccgaacagt cggatggcgg cgggaacgac atagagccgt tttgatgctg tagcgatgcg
     3601 gcgaacggcc gcctccgcct cctgtatctc cttctcggtg aaccatgcat cggggaggcg
     3661 tcgcgcgctc ttttcaagca tccgaccgaa gtcggcctca tattcgtatg cggtgccgcc
     3721 gatggtcagg gtgacggttc ggaaggggtg cgtcgcgtcg aagatcattc ccggggtcgg
     3781 tcgaccgtcc agttcgatgc gggcgtcggg cacgaggtag ccgtcgcgcg acacgatccg
     3841 ggcgaggagt cgcggcgtcg tccgcaggtc gtcgccgagc agatacaggt ggtaatcgcg
     3901 tttttcgccg tgcagccaga gaaatacgct gacgagcgcg aagatgaccg cgagaaagga
     3961 tacgatggct atcgccgtcc gtttcgcatc ggtgtcgccc ggtccgttca tcgagaggtc
     4021 ctctccgcag gatggggcat tataggtgtc ccgccggcgg gaggcaattt tttacttttc
     4081 cgcgggaaag aagagaaagc gtgtcgaccg gatcgtcggt gcgcgccgcg gttccttgct
     4141 aaatatcctt aatgttctta gtgtgttgat ctttgaagag ccatccctct ctaaaaatgc
     4201 caaaaaaaat tgaaaacggg gctgtcgccg cttatagtgc ctgtgccgaa aacctttgtt
     4261 ccacataagg aggaatgcat gagttttgaa tcgacgaggg tcaaggagat cctcaaggac
     4321 tatgcgcccg aaaagggcaa cctgatggcg gtgttgcgcg aactccagaa ggagggcggc
     4381 aaccacctgt ccgatgaaac cctcaaagag gtggtccgga ccatcggcat cccgctgaac
     4441 cggctctacg gcttcgtgtc gttctacacg atgctctcga ccgcgccgaa gggacgccac
     4501 atcatccgcg tctgcaagga cggcccctgc aacttcgcgg gcggctgggc gacggccgaa
     4561 ctgctcgaga aggaactcga catcgagatg ggcgaaacga ccgaggacgg ccgtttcacg
     4621 ctcgagcaca ccgcctgcct cggcacctgc acggcggcgc cggcgatcat gatcgacgat
     4681 gagacctacg ccaacgtgac tcccgagaag ctcggcgaga tcctgaagaa gtacaagtaa
     4741 gggggacgta atgaactaca acgcgaagat actttccgcc gacaaggacc atgtgctcct
     4801 caagaacgtc ggcaagatcg atccgatgag catcgacgcc tacgtcaagg cgggcggtta
     4861 tgaaggactc aaaaaggcgc tcgcgatgga cccgaagaag gtcatcgacg aggtgagcga
     4921 atcgggactg cgcggccgcg gcggcgccaa tttcccgacc ggccgtaaat ggaccttcac
     4981 cgccggcgcg aaggccgacc agaaatacat catctgcaac gccgacgaag gcgaaccggg
     5041 caccgtgaag gaccgcatcc tgatggaagg cgacccgcac cagatgatcg aagggatcgt
     5101 gatcggcgcc tacgccatcg gcgcgaccaa gggcttcatc tacatccgcg gcgaataccg
     5161 caaatcgata gagaccatcg agaaggcgat cgccgacgcc gagaagaaga acttcctcgg
     5221 taaaggcatc ctcggctcca agttcgattt cgagctccgg gtggtcgaag gcggcggcgc
     5281 gtatgtctgc ggcgacgaga cggcgctcat cgaatcgatc gaagggaagc gcggcgagcc
     5341 gcgcatgaaa cccccgttcc cgccggtggt gggcttgtgg ggcaaaccga ccatcgtcaa
     5401 caacgtcgag accctcgcca acgtcccgca catcatcaag aacggcgcgg cgtggttcct
     5461 caagaagggt ccggccggtt cgccggggac caagatattc atgctcgtcg gcgatgtcaa
     5521 cgagcccggc gtggtcgaga ccaccttcgc gcaacaaccg atgcgcgtgt ttcgcggcgt
     5581 g
//
LOCUS       DAOQUE010000148         5565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16064_length_5565_cov_4.178040, whole genome shotgun sequence.
ACCESSION   DAOQUE010000148 DAOQUE010000000
VERSION     DAOQUE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16064_length_5565_cov_4.178040"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PKH10_05825"
     CDS             <1..680
                     /locus_tag="PKH10_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27683.1"
                     /translation="ARLIEVTIESGTFISTPVVGGNCYEIEGTWDTICVPDCGDKVCG
                     SDGCGGVCGDGCEEGKQCNAEGTGCIDCTPVSAKDIVGDPEMANLYVGGLVDNIGTSV
                     ADSFYLEFWGAQEAGTYDLTGTNYVDCPQCVLVVQDLGDTALQKVFFQHSGDLVIDDL
                     VVDEEDWMTGASRGHLDGVRLIEVVINGETYESTPVAGGACLQIVSAAWDTMPEETDE
                     DAILPDN"
     gene            complement(699..2498)
                     /locus_tag="PKH10_05830"
     CDS             complement(699..2498)
                     /locus_tag="PKH10_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27684.1"
                     /translation="MRGVMFLFAFLSFLLVSCDNQVAQRPEAHSDQDTVGPDDIGDFP
                     LLADEWQNDVPPTESEAPLADQDPFMPPDDTTDNASDDTADTVPDDIPDAVPDVATDE
                     TTDHGPDETTDEVPDEVPDEMNDETSDSMPEEDPDLDEAVIDDAPVEDDPLSDDATVL
                     PDLDLVPFDEDMAPDLDILPEFDTPPETDWSFPDIDLPLPEYDMLPEFDILPEYDLLP
                     EPDDVLPESDLLPEADIVTPDPDQPLPDPDTVTPDIDIVPDACTVDGDCAFGYRCDTT
                     TSPRACLFASTCANDFDCQTYQTCQVVGNWKECRLDLSDSCETDADCAPGEVCETVIL
                     GYKVCRSMNKCLTSEECAANEVCDWTGSYYDCVAVCTVDTDCAFGYQCIAGTPYNRCE
                     YANECQSDADCPAFRTCEPSGNWMQCILSLGGGLCLNDTNCDPSEYCDLSLGFFGTCK
                     SRDQCLIDADCGDNMKCEFNGTYYECVPTNPKQCLFDFQCPTDWICAQNVCTPQYAGT
                     CTEIEGLWNVLVSTALFFTTGSSYEFIPKDGCDGSIKQEGGTVSSGTFAQTSAFNYDI
                     TMLLFFNCQASITLNAIMQVTCSSGSATLIRSN"
     gene            2617..2692
                     /locus_tag="PKH10_05835"
     tRNA            2617..2692
                     /locus_tag="PKH10_05835"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2650..2652,aa:Gly,seq:gcc)
     gene            2716..2789
                     /locus_tag="PKH10_05840"
     tRNA            2716..2789
                     /locus_tag="PKH10_05840"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2748..2750,aa:Cys,seq:gca)
     gene            2811..2899
                     /locus_tag="PKH10_05845"
     tRNA            2811..2899
                     /locus_tag="PKH10_05845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2845..2847,aa:Leu,seq:taa)
     gene            2934..4049
                     /locus_tag="PKH10_05850"
     CDS             2934..4049
                     /locus_tag="PKH10_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNT27685.1"
                     /translation="MNDPRLDKLAKVLVHYSTRVKKGEFVFVRADEVAAPWVVAVARE
                     ATRAGAHVEVMLSSQEATEARLKYASDEELKTGNYMLEKMLERADVWLTAWAARNVKV
                     NAAIPAERLKLDAQGQGGWRKIYSDRMASGKLRWCGTQFPTYADAQEANMSLEDYEDF
                     VYGAGLLDKDDPAAAWKKVSESQERWVKWLDTKRSLHIVSEGTDITVGVAGKKWINCD
                     GRVNFPDGEVFTSPEDTNINGHITFSFPGIYAGKEIEGIRLEVKDGLVVKATARKGEE
                     LLQALLKTDEGASRFGEVAVGTNYGIQRFTRNMLFDEKIGGTVHMALGNSMAEAGGKN
                     HSAIHWDMLCDMRQGGTITADGKLFYKDGKFIDEVLV"
     gene            complement(4051..4398)
                     /locus_tag="PKH10_05855"
     CDS             complement(4051..4398)
                     /locus_tag="PKH10_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27686.1"
                     /translation="MYEFGKMLGWVNIALLLAAVAIFPARKFLKPPSGLLAFLRRIHP
                     WAAGLLVVSAMTHSFIVWGVPQFNSGQPLLGAIILAMLLAWVGPKLKLPLWFKLHLVM
                     PIVVIALLVWHLW"
     gene            complement(4407..4634)
                     /locus_tag="PKH10_05860"
     CDS             complement(4407..4634)
                     /locus_tag="PKH10_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNT27687.1"
                     /translation="MFTLEELAKFDGKEGRPAYVAINGIVYDVTGVKAWAKGEHGNGK
                     PGTDITPLITTKSPHGDKVTKNLKAVGKLKK"
     gene            complement(4755..5435)
                     /locus_tag="PKH10_05865"
     CDS             complement(4755..5435)
                     /locus_tag="PKH10_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27688.1"
                     /translation="MKRVLLFAAILIISTALCAAGEEQKKETEKEKDRSFLLALSVPF
                     LDYTYIKEQDNFFAYGDDLLSLGLELTSLDTAFGFRLTDMFYLMGRFGFGIKWAGATG
                     ERPRPQNSFLIFGVGPRLAFEARNLLVNVGIYLNYRYDDGQQVQMGPVETGINYGEDR
                     GHTLALEGYFGVEYLVLDNFAVGGKLSLGYLHIWREEAIHDEDDFVRKTEFNGVDLGI
                     HISTSIFF"
BASE COUNT         1151 a         1488 c         1785 g         1141 t
ORIGIN      
        1 cggcgcgtct catagaggtg accatcgaga gcggtacctt catatcgacc ccggtcgtcg
       61 gcgggaactg ctacgagatc gaggggacat gggacaccat ctgtgttccc gactgcggcg
      121 acaaggtctg cggctctgac gggtgcggcg gcgtgtgcgg cgacggttgc gaagaaggca
      181 aacagtgcaa cgccgaaggt accggatgca tcgactgtac gccggtgagc gctaaggata
      241 tagtcggcga tcccgagatg gccaacctct atgtcggcgg tctggtcgac aacatcggga
      301 cctccgtcgc cgattcgttc tatctcgaat tctggggtgc acaggaggcg gggacctacg
      361 atctgaccgg caccaactat gtcgattgcc cacagtgcgt ccttgtcgtg caggatctcg
      421 gcgacacggc gctccagaag gtcttcttcc agcattccgg cgatcttgtt atcgacgatc
      481 tggtcgttga cgaggaggat tggatgaccg gcgcctcgcg tggacacctt gacggcgtgc
      541 gactgataga ggtcgtcatc aatggcgaga cctacgaatc gaccccggtg gctggcggtg
      601 cctgtctgca gatcgtttct gcggcgtggg atacgatgcc ggaagagacc gacgaggacg
      661 cgatacttcc cgataactga tcggcccggg ttcccgggtc agttgctccg tatcagcgtt
      721 gcgctaccgc tcgagcaggt gacctgcatg atggcgttca gggttatcga ggcctgacag
      781 ttgaagaaca acagcatggt gatgtcatag ttgaaggccg aggtctgtgc aaaggtgccg
      841 ctcgaaacgg tacctccctc ctgttttatg gaaccgtcgc agccgtcctt cgggatgaat
      901 tcgtaggagg aaccggtggt gaaaaagagc gccgtcgaaa cgagaacatt ccaaagcccc
      961 tctatctcgg tgcaggttcc ggcgtactgc ggcgtgcata cattctgggc gcagatccag
     1021 tcggtagggc actggaagtc gaacaggcac tgtttagggt tcgtcggcac acattcgtaa
     1081 taggtcccat tgaattcgca tttcatgttg tcgccgcagt cggcgtcgat aaggcactgg
     1141 tcacgcgatt tgcaggtccc gaaaaagccg agtgaaagat cgcagtattc gctcgggtcg
     1201 cagttcgtgt cattcaggca gaggccgccg ccgagtgaga ggatgcactg catccagttg
     1261 cctgacggtt cgcaggtccg gaaggcgggg caatcggcgt cgctttggca ctcgttggcg
     1321 tactcgcacc ggttgtaagg ggttccggcg atgcattggt agccgaacgc gcagtcggtg
     1381 tcgaccgtgc agaccgcgac gcagtcgtag taggagccgg tccagtcaca gacctcattc
     1441 gcggcgcatt cttcgctcgt gaggcatttg ttcatggagc ggcagacctt gtagccgaga
     1501 atgaccgtct cgcagacctc gcccggcgcg cagtcggcat cggtttcgca ggagtcgctg
     1561 aggtccaagc gacattcctt ccagttgccg accacctgac aggtctggta ggtctgacag
     1621 tcgaaatcgt tggcgcaggt gctcgcaaag aggcaggcgc gaggtgatgt cgtggtatcg
     1681 cagcggtatc cgaaggcgca gtcgccgtca acggtgcatg cgtcgggcac aatgtcgatg
     1741 tcgggtgtga cggtatcggg gtcggggagc ggttggtcgg gatccggcgt cacgatgtcg
     1801 gcctccggca ggaggtcgga ttcggggaga acatcgtccg gctcaggcag gaggtcgtac
     1861 tcgggcagga tatcgaattc ggggagcata tcatactcgg ggagcggaag gtcgatatcg
     1921 gggaaggacc agtcggtctc aggcggggta tcgaattccg gaaggatgtc gaggtcgggg
     1981 gccatatcct cgtcgaaggg aacaaggtcg agatcgggca gaacggtcgc gtcatcggag
     2041 agcgggtcat cttcgacggg agcatcgtcg atgaccgcct catcgaggtc gggatcctcc
     2101 tccggcatgc tgtcgctggt ctcgtcgttc atctcgtcgg gaacctcgtc gggaacctca
     2161 tcggtggtct cgtccggtcc atggtcggtc gtttcgtcgg tcgccacatc ggggaccgcg
     2221 tcggggatat catcgggtac ggtgtcggcg gtgtcgtccg acgcgttgtc ggtggtgtcg
     2281 tctggcggca tgaaggggtc ctgatcggcg aggggagcct cgctctcggt cggagggacg
     2341 tcgttctgcc actcgtcggc aagcagaggg aagtcaccga tgtcgtcagg gcctacggtg
     2401 tcctggtcgc tgtgcgcctc ggggcgctgc gccacctgat tatcgcagga aacgagcaga
     2461 aacgataaaa aggcgaacaa gaacatcacc ccacgcatgg cgctcccctt ttgttgtatg
     2521 ggcatcctga ttgtacttaa aggtttagcg tttgcaagga gataaaattt gttgacagcg
     2581 gtcgaaaagt cggtataaaa aaagcgcttg tgctgagcgg gaataactca gtggctagag
     2641 tgcaaccttg ccaaggttga ggtcgagggt tcaaatccct tttcccgctc catttttttt
     2701 cttcttctta tccggggcgc cttggccaag tggtaaggca gaggtctgca aaatctccat
     2761 ctccggttcg aatccggaag gcgcctccat ccttgccaga tagttgttgt gccggagtgg
     2821 cggaattggc agacgcaagg gacttaaaat ccctcggggc gcaagccctg caccggttcg
     2881 accccggtct ccggcaccat catgcaagaa acctttagca taggaggatc atcatgaacg
     2941 atccgagact cgacaaactg gcgaaggtgc tcgtccacta ttcgacccgc gtgaaaaagg
     3001 gagagttcgt ttttgttcgc gccgacgagg tcgcggcgcc atgggtggtc gcggtggccc
     3061 gcgaagcgac ccgggcgggc gcccatgtcg aggtgatgct ctcgtcgcag gaggcgaccg
     3121 aggcgcgtct caagtacgca tccgacgagg aactgaagac cggcaactac atgctggaga
     3181 agatgcttga gcgggccgat gtgtggctga cggcatgggc ggcgcgcaac gtgaaggtca
     3241 acgcggcgat accggccgaa cggctcaaac tcgatgcgca ggggcagggc ggctggcgca
     3301 agatatattc cgatcggatg gcgtcgggga aactgcgctg gtgcggcacc cagttcccga
     3361 cctatgccga cgcgcaggag gccaacatga gtctcgagga ctacgaggat ttcgtctacg
     3421 gcgcgggtct gctcgacaag gatgatccgg cggccgcatg gaagaaggtc agcgagtcgc
     3481 aggaacgctg ggtgaaatgg ctcgacacga agagatcgct tcacatcgtc tccgagggga
     3541 ccgacatcac cgtcggcgta gcgggcaaga agtggataaa ctgcgacggc cgggtcaact
     3601 tccccgacgg cgaggtattc acgagccccg aggataccaa tatcaacggg catatcacct
     3661 tcagttttcc cggtatctat gcggggaaag agatcgaggg gatacggctc gaggttaaag
     3721 atgggctggt cgtcaaagcg acggcgcgga aaggcgagga actcttgcag gcgcttctca
     3781 agaccgacga gggggcgtcg cgattcggcg aggtggcggt cgggaccaac tacggcatcc
     3841 agcgcttcac gcgcaacatg ctcttcgacg agaagatcgg cggcaccgtc cacatggcgc
     3901 tcggcaattc gatggccgag gcgggcggga agaaccattc ggcgatccat tgggatatgc
     3961 tctgcgatat gcgacagggc ggcacgataa cggccgatgg gaaactcttc tacaaggacg
     4021 gcaagttcat cgacgaagtg ctcgtctagt tcaccacaaa tgccagacaa gtagcgcgat
     4081 cacgacgatc ggcatcacga ggtgcagttt gaaccagagc ggcagtttga gtttcgggcc
     4141 gacccacgcc agaagcatcg cgaggatgat cgcgccgaga agcggctgac cgctgttgaa
     4201 ctgcggcacg ccccagacga tgaacgaatg ggtcatcgcc gaaacgacga gtagccccgc
     4261 cgcccacgga tggatgcgtc gcagaaaggc gaggaggccg ctgggcggtt tcaggaattt
     4321 ccgggccggg aagatagcga ccgccgcgag gagcagggcg atgttcaccc agccgagcat
     4381 tttaccgaat tcgtacatga tctccctcac ttcttgagct taccgaccgc cttgaggttc
     4441 ttggtcacct tgtcaccgtg gggtgacttc gtggtgatga gcggggtgat gtcggtgccg
     4501 ggtttaccgt taccatgttc gcccttggcc cacgccttga cgccggtcac atcatagacg
     4561 ataccgttga tggcgacata ggcggggcgt ccttccttgc cgtcgaattt ggcgagttct
     4621 tcgagggtga acaccttttc ggccgtcgca ggcgccggtt tcgccggttc gaccggtttc
     4681 ttttcttggg caatgacggt gaatgccgta aaggcgacaa ggacgagaat aagtcttttc
     4741 atgcggtcct ccttttaaaa aaatatgctg gtgctgatat gtatcccgag atcgacgccg
     4801 ttgaattcgg tcttgcggac gaaatcgtcc tcgtcatgaa tggcctcttc tctccatatg
     4861 tgcaaatagc ccagagataa ttttccgccg acggcgaagt tgtcgagtac cagatattcg
     4921 acgccgaagt acccttcgag tgcgagggtg tgccctcgat cctcgccata gttgattccg
     4981 gtctcaaccg gtcccatctg gacctgctga ccgtcgtcat agcgatagtt caggtatatc
     5041 cccacattga ccagtaagtt gcgtgcttcg aaggcgagcc gcggtccgac tccgaatatc
     5101 aggaatgaat tctgtggccg tggccgttcg ccggtggcgc ctgcccactt gatgccgaaa
     5161 ccgaagcggc ccatcaggta gaacatgtcg gtcagccgga agccgaaggc ggtgtccaaa
     5221 gaggtcagtt ctagaccgag cgaaaggaga tcgtcgccgt aagcgaaaaa gttatcctgc
     5281 tccttgatat aggtatagtc gaggaagggg accgaaagag caagaagaaa cgagcggtcc
     5341 ttttccttct ccgtttcctt tttttgttcc tctccggcgg cgcagagggc cgtcgagatg
     5401 atcaggatag cggcgaagag caacacccgt ttcataaggg ctccctcgga tggtccgtaa
     5461 cacccgttca tgatacccga tgagtgcccg gattcaattt ttccaaaaat tgttgaagca
     5521 aagtcaaaat gtccccctaa ctgcaaagcg attatgtccc ctagg
//
LOCUS       DAOQUE010000149         5563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16076_length_5563_cov_4.260893, whole genome shotgun sequence.
ACCESSION   DAOQUE010000149 DAOQUE010000000
VERSION     DAOQUE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16076_length_5563_cov_4.260893"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PKH10_05870"
     CDS             complement(<1..1177)
                     /locus_tag="PKH10_05870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT27689.1"
                     /translation="MSPERKRAVAVAAVFLCSLVAYGAIAWWTYRFPIISYDEAAYSA
                     TARRLATEGGWLRLYGSGDLFFFPPLFNYLAGGLIMLGIERLAAVRFVAVLAGAGVNA
                     FFFALVRRHVGDRAAWIAVLWAFFMPIKASYSAVGQVELPMLCAVLAACYVAFSDIEE
                     RWRAAGTAVLLAAGVWIKETAIGFIPLFVVVWFIRGDRRLAVRVGVLSLLLLSPLFAQ
                     SFFPHEYDLFYEVTTPFIRFSGLQPDMMLMNLLRQFAVDPLRLFGFGWAIAAAFVVLT
                     VFSFAHLRRRSVWADPFLAFSVLGVILWGSFFLYFPRKFDYYLIPLSMLLLVPVSCFL
                     AKRTVLAKVATGTMIALSLWALWGFVSFRDETAALRDILARAADEMPGARVAMTLPRS
                     "
     gene            complement(1179..2228)
                     /locus_tag="PKH10_05875"
     CDS             complement(1179..2228)
                     /locus_tag="PKH10_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT27690.1"
                     /translation="MSIVAVIMAGGSGKRFWPLSREDKAKQSLALFSDRTLLGETITR
                     LLPLTDNILVVSSAKQRRHIEADIAAFPGVRAIFEPVGRNTAPCIALALRAIGEFWGG
                     DDRVAVLPADHHIADPEEFRATMTRCLAVLEKHDGIGTIGMRPTYPETGYGYIHIGAS
                     LADGVFAVERFEEKPKRERAEEFVRSGRYLWNGGIFLFRASTMRRELKTQVPDIMTAV
                     ETLPDPIAVTKEQYGAIRSISIDYAVMEKTALPICCALGDFGWSDVGGWASYRDLLPK
                     DEHGNAIRGPAHLIDCRDNLVVNLTARPIFLLKKQGQLVVATDDALLCADLADHQEVR
                     RVTEYLEKHGLAALL"
     gene            complement(2264..2350)
                     /locus_tag="PKH10_05880"
     tRNA            complement(2264..2350)
                     /locus_tag="PKH10_05880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2314..2316),aa:Leu,seq:caa)
     gene            complement(2390..2794)
                     /locus_tag="PKH10_05885"
     CDS             complement(2390..2794)
                     /locus_tag="PKH10_05885"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HNT27691.1"
                     /translation="MAKPVIPVTAAIIVRRGKVFIARRADKGDTRGKWEFPGGKIEPG
                     ETPQECLARELCEEFGICVTVGDLFVEHEHDYGARVVRLMVFRVTTVTGRFVPTEHSA
                     MAWVPPAELLSYDLAPADIPVAERLAAVPRKN"
     gene            2908..3693
                     /locus_tag="PKH10_05890"
     CDS             2908..3693
                     /locus_tag="PKH10_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT27692.1"
                     /translation="MDTVQKKKERSNLEGKAFLNLHKRVETAIMDYDMIHPGDHIMVG
                     LSGGKDSSLLLALLARKKIETTNDFKLSAIFVRMGFTGDDEKLAYLKDYAMSLGVPFH
                     AIEEPIFEAFKKEKKKPCYVCSRNRRIAMFRYAKEIGANVIAFGHHRDDFMQTLLLNI
                     LYGSCVETMKPNNPFFGGEFRVIRPMLYIDEKMITAEVKRRSIEVFPQGCPFSCMSER
                     AYVKELITEAHKRHRRVRQSIFRSMFTVRPEYLLKPPSRKSML"
     gene            3704..4777
                     /gene="wecB"
                     /locus_tag="PKH10_05895"
     CDS             3704..4777
                     /gene="wecB"
                     /locus_tag="PKH10_05895"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012666568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNT27693.1"
                     /translation="MKVLVSIGTRPEFIKMVPVLRELKKRGVENHFLYTGQHRDLCRP
                     LFDFFAIHPDTDLDIMKENQPIAHITSSILTRLGTVLDTVEPDIVIVHGDTSTTLASS
                     LAAFYKKIPVAHVEAGLRSHDRYSPFPEEMNRVLTDRLASHHFAPTTLNRDNLLREGI
                     DPATIFVTGNTVIDTVKLVAAKIKAEKKKQVLITAHRRENFGEPMEHICRAIAELARL
                     FPDRAFLYPVHPNPNVRKTTDALLTGISNVRLVEPLDYIAFITEMASSELVLTDSGGI
                     QEEAPAFGIPVVVMRRETERTEGVEAGTLILAGTAQSDIIAAAKRLLTDSAYYASFAN
                     ATNPYGDGHAAERIVQKLFEISF"
     gene            complement(4785..>5563)
                     /locus_tag="PKH10_05900"
     CDS             complement(4785..>5563)
                     /locus_tag="PKH10_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27694.1"
                     /translation="TDDTVVDNETPDDDAAPSYNFNGKWAMLNFTTSNVETDLGGILG
                     TQKSTSTTSSYFLVELTDNGDGSVGAVGTACETLIDSGSNLLTVTISDNYIAHVKPMI
                     WDFTTILDRGGVGFDVTASDILTLNGVKDLADPLNDPLPTDKSDTHIYDQDDDTNPGM
                     TVNCTGNIPMVFNGPGTMYTIQRTVNTMVGESKDDMRIEGALAWESEQVVLEASNSVL
                     LNDKTVTPDLANSWFKMVRLIDQNWTCAEIKANKDTLFPQ"
BASE COUNT         1197 a         1761 c         1596 g         1009 t
ORIGIN      
        1 ccgatcgcgg cagggtcatg gcgacgcgcg cccctggcat ctcgtcggcg gcccgcgcaa
       61 ggatgtccct cagcgcggcg gtctcatcac ggaacgaaac gaagccccag agcgcccaga
      121 gcgaaagcgc gatcatcgtc ccggtagcca cctttgcgag gaccgttcgt ttcgcgagga
      181 aacaggagac agggaccagc aagagcatcg acagcgggat aaggtagtaa tcgaatttgc
      241 gcgggaagta gaggaaaaag gatccccaca ggatcacccc gaggaccgaa aaagcaagga
      301 acggatccgc ccaaacgctt cttcttctca aatgtgcgaa cgaaaacacc gtcaggacaa
      361 caaaggccgc agcgatcgcc caaccgaacc cgaacaggcg gaggggatcg acggcgaact
      421 gacggagaag attcatgagc atcatgtcgg gttgcagccc cgaaaaacgg atgaacgggg
      481 tggtcacctc gtagaaaagg tcatactcat gcgggaagaa actctgcgca aaaagcgggg
      541 agagcaggag gagcgacagc actccgacgc gcacggccag tcgccgatcc cctcgtatga
      601 accatacgac gacgaacagc gggatgaacc cgatcgcggt ctccttgatc cagacccccg
      661 ccgctaggag aacggcagta ccggcggcac gccaccgctc ttcgatatcg gaaaaggcca
      721 catagcaggc ggcaagtacc gcgcacagca tcggcagttc cacttgtccc accgctgaat
      781 aggatgcctt gataggcatg aagaaggccc acaggaccgc gatccatgcc gcgcgatcgc
      841 cgacatgccg ccgcaccaga gcgaaaaaga aggcgttcac cccggcgccc gcaaggaccg
      901 cgacgaaacg caccgccgca agccgctcaa tgccgagcat gatgagcccg ccggcgagat
      961 agttgaagag cggcgggaaa aagaacagat caccgctccc gtagagccgt agccaaccgc
     1021 cctccgtcgc gaggcgtcgc gccgtcgccg aataggccgc ctcgtcgtaa ctgatgatcg
     1081 ggaaccggta ggtccaccac gcgatcgcac cgtaggcgac aagcgaacag aggaacactg
     1141 cggcgaccgc gaccgcccgt tttctttcgg gactcatatc ataacagcgc ggcgagcccg
     1201 tgcttttcga gatattcggt cacccgccgc acctcctgat gatcggcgag gtcggcgcag
     1261 agcagggcgt cgtcggtagc caccaccagc tgcccctgtt tcttcaacag gaatatcggc
     1321 cgggcggtca ggttgacgac cagattgtcg cggcaatcga tgaggtgagc cggtccacgg
     1381 atagcgttgc cgtgttcgtc cttcggcaac agatcgcggt aactcgccca gccgccgaca
     1441 tcgctccagc cgaaatcgcc gagagcgcaa cagatcggca gagcggtctt ttccatcacc
     1501 gcgtagtcta tcgagatgga gcggatggcg ccgtactgtt ccttggtgac cgcgatggga
     1561 tcagggagcg tttcgaccgc cgtcatgatg tcgggaacct gcgtcttgag ttcgcgtcgc
     1621 atggtcgagg cacggaacag gaatatgccg ccgttccaga gatacctgcc gcttcttacg
     1681 aattcctcgg ctcgttcgcg cttcggtttc tcttcgaacc gttcgaccgc gaacaccccg
     1741 tcggccaacg acgcgccgat gtggatatag ccgtagccgg tctcgggata ggtcggacgc
     1801 atcccgatgg tgccgatgcc atcgtgtttt tcgagcaccg ccagacaccg tgtcatcgtc
     1861 gcacggaact cttcgggatc tgcgatgtgg tgatcggcgg gtagcaccgc cacccgatca
     1921 tcgccgcccc agaactcccc tatcgcccga agcgcaagcg cgatgcaggg cgccgtgttc
     1981 cggccgaccg gctcgaagat ggcgcggaca ccgggaaagg cggcgatatc cgcttcgatg
     2041 tggcgacgct gtttggcgga cgaaacgacg aggatattat cggtcagcgg caggaggcgc
     2101 gttatcgtct cgccgagcag ggtccggtcg gaaaaaaggg cgagcgactg tttcgccttg
     2161 tcctcgcgcg aaagcggcca gaaccgcttg ccgctcccgc ccgccatgat aacggccacg
     2221 atcgacatcg ccgcaccccc tatcggtcgc tgttagaaaa gactggtgcc ggaggtcgga
     2281 cttgaaccga cacggtgtca ccaccaccgg attttgagtc cggcgcgtct gccaatttca
     2341 ccactccggc actgataacg ggggcattct atggcccgac ggggggttgt caattttttc
     2401 gcgggacggc ggcgagccgt tcggccacag gtatgtcggc aggcgcaaga tcataggaga
     2461 gcagttcagc gggggggacc cacgccatgg cgctgtgctc ggtcggcacg aaacggcccg
     2521 ttaccgttgt gacgcggaac accatgagcc ggaccacccg cgcaccgtaa tcgtgctcgt
     2581 gttcaacgaa cagatccccc accgtcacgc agattccgaa ctcttcgcac agttcgcggg
     2641 caagacattc ctgcggcgtc tcgcccggct ctatcttgcc accggggaat tcccacttgc
     2701 cacgggtatc gcccttgtcg gcgcggcgcg cgatgaatac cttcccgcgc cggacgatga
     2761 tcgcggcggt gacgggtatg accggtttcg ccatctgttc ctcctttcgc ggcagtatgg
     2821 caccacgatg gatcttttgc aatttttacc tctcctttac aaatcatcgc cgctccggta
     2881 taagtgtgac gtaacacacg gggtcgcatg gataccgtac agaaaaagaa ggaacgatcc
     2941 aacctggagg ggaaggcgtt cctcaacctc cacaagcggg tcgagaccgc catcatggat
     3001 tacgacatga tacaccccgg tgaccatatc atggtggggc tgtcgggcgg caaggattcg
     3061 tcgctgctac tggcgctgct cgcccgcaag aagatagaga ccaccaacga cttcaagctc
     3121 tccgccatat tcgtccggat gggattcacg ggcgatgacg agaaactggc ctatcttaaa
     3181 gattacgcca tgtcgctcgg cgtccccttc cacgccatag aggagccgat attcgaggcc
     3241 ttcaaaaaag agaagaaaaa gccctgctat gtctgttcgc gcaaccgccg catcgcgatg
     3301 ttcagatacg ccaaggagat cggcgccaat gtcatcgcct tcggtcacca ccgcgacgac
     3361 ttcatgcaga cgctcctgct taacatcctc tacggctcct gcgtcgagac gatgaagcca
     3421 aacaacccct ttttcggcgg tgagttccgc gtcatccggc ccatgctcta catcgacgaa
     3481 aagatgataa cggccgaagt gaagcgccgc agtatagagg tcttcccgca ggggtgcccc
     3541 ttctcgtgca tgtcggagcg tgcctatgtg aaggaactca tcaccgaggc gcacaaacgg
     3601 caccgccgcg tccggcagag catattccgg tcgatgttca ccgtgcggcc cgagtacctg
     3661 ctcaagccgc ccagccgcaa aagcatgctg taggagaaaa gccatgaagg ttctcgtatc
     3721 gatcggcacc cgccccgaat tcatcaagat ggtgccggtc ctccgcgaac taaaaaaacg
     3781 cggcgtcgaa aaccatttcc tctacaccgg ccagcaccgc gatctctgtc ggccactgtt
     3841 cgatttcttc gccattcacc ccgataccga cctcgatatc atgaaggaga accagccgat
     3901 agcccatatc acgagttcca tcctcacgcg gctcggcacg gtgcttgaca ccgtcgagcc
     3961 cgatatcgtg atcgtccatg gcgacacctc caccaccctt gcctcgtcgc tcgccgcctt
     4021 ctataaaaag atcccggtcg cccatgtcga ggcgggactg cgcagccatg accgctactc
     4081 accgttcccc gaggagatga accgggtgct caccgaccgg ctcgcttcgc accacttcgc
     4141 cccgacgacg ctcaatcgcg acaacctcct gcgcgaaggg atcgacccgg cgacgatatt
     4201 cgtcaccggc aataccgtca tcgacaccgt gaagctcgtc gcggcgaaga tcaaggcaga
     4261 aaagaagaaa caggtgctca tcaccgcaca ccggcgcgag aacttcggcg aaccgatgga
     4321 gcacatctgc cgcgcgatag ccgaactggc gcgccttttc cccgaccgcg cgttcctcta
     4381 cccggtccac ccgaacccga atgtccgcaa gacgaccgac gcgctgctca ccggcatcag
     4441 taatgtccgc ctcgtcgagc cgctcgacta tatcgccttc atcaccgaga tggcgtcgtc
     4501 cgaactggtc ctcaccgatt ccggcggcat tcaggaggag gcgcccgcct tcggcatccc
     4561 cgtcgtcgtg atgcggcgcg agaccgaacg gaccgaaggg gtcgaggcgg ggacgctcat
     4621 cctcgccggg accgcgcaga gcgacatcat cgcggcggcc aagcggctcc tcaccgactc
     4681 cgcttactac gcctccttcg ccaacgcgac caatccctac ggcgacggcc acgccgccga
     4741 acggatagtg caaaaactgt tcgagatatc tttctgaaac gggcctactg cgggaacagc
     4801 gtatccttgt tcgcctttat ctcggcgcag gtccagttct ggtcgataag acggaccatc
     4861 ttgaaccaac tgttggccag gtcgggcgtc acggtcttgt cattcaaaag gacactgttc
     4921 gacgcctcga gaaccacctg ttcgctctcc catgccaacg cgccttcgat acgcatgtcg
     4981 tccttgctct cccccaccat ggtattgacg gtgcgctgaa tagtatacat cgttccgggg
     5041 ccgttgaaga ccatcgggat attaccggta cagttgaccg tcatgccggg attggtgtcg
     5101 tcgtcctgat cgtagatatg tgtatcgctc ttgtcggtcg ggagcgggtc gttgagcgga
     5161 tcggcaagat ccttaacccc gttgagggtc aggatgtcac tcgcggtgac atcgaacccg
     5221 acgccgccac ggtcaagtat cgtcgtgaag tcccagatca tcggcttgac atgggcgatg
     5281 tagttgtcgc tgatggtgac ggtcagcagg ttcgatccgc tgtctatcag ggtctcacag
     5341 gcagtaccga ctgctcccac actaccatca ccgttatcgg tcaactcgac cagaaaataa
     5401 gaactggtgg tcgaggtgct cttctgcgtg ccgagtatcc cgcccagatc ggtctccaca
     5461 ttcgaggtgg tgaagttgag catcgcccat ttaccgttga agttgtagga aggagcggca
     5521 tcatcgtcgg gcgtttcgtt gtcgaccacg gtgtcatcgg taa
//
LOCUS       DAOQUE010000150         5563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16077_length_5563_cov_4.094953, whole genome shotgun sequence.
ACCESSION   DAOQUE010000150 DAOQUE010000000
VERSION     DAOQUE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16077_length_5563_cov_4.094953"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKH10_05905"
     CDS             complement(<1..405)
                     /locus_tag="PKH10_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HNT27695.1"
                     /translation="MHRTMRASLDDLFLFLDGSPTMFHATVSIIGRLEKAGFSRLEEG
                     DEWSLKSGAGYFVVRNDSAVIAFRIGRKSPWESGFMMAGAHTDSPLLKIKPEAESMGK
                     NVVRVATEIYGGPIVATWLDRNLAVAGRVMVKG"
     gene            complement(422..1954)
                     /locus_tag="PKH10_05910"
     CDS             complement(422..1954)
                     /locus_tag="PKH10_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27696.1"
                     /translation="MGRSCVWRLILWATLASSLPLAGENDGVVEEKDTVSPVTTITEK
                     PESPTLERSALFRFTASEPVSRYECRLDDELWHRCTGEERYEALSEGTHTFTVRAIDR
                     AGNVEAKPTVFEWEIPYQFVHVSTGYQHTCAVNAGGKPHCWGNNNAQQLGISDRQESF
                     YPVEPRNLIAWWRKVSAGRFHTCGIGGKDRSDQRVYCWGSNGSGQLGVGDERSGFVPM
                     EVAGGYDDWEQMTAGISHTCGIRTDGGKGTLYCWGENGAGQVGDPSYQRALSPRPVAG
                     DLRGWRYVSGGFSHTCGLREDGGENRVYCWGANGDGQLGDGSTEKRRAEPVEVGGGHV
                     DWESVSAGYYHTCGIRNEQGVRRIYCWGSAGSGQLGTGDTAKRTTPTPIAGDNDSWLS
                     LTAGANHTCAIRQEGEGRAIYCWGDNRYGQSGLGRLDRVAAPQKVKSDHADWQQMSAG
                     ETHTCAIREHKKRGYLYCWGRNQFGQLGDGTDETRTTPVEITGMEQKKAAASAKRTDS
                     HP"
     gene            2110..2442
                     /locus_tag="PKH10_05915"
     CDS             2110..2442
                     /locus_tag="PKH10_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HNT27697.1"
                     /translation="MRELCRLKEIFRAIYVFETTLKKRFRLSLNEALALCALEQGEKN
                     SRELAQEIGISFSRMSRVLGSLEEKGLIKRTFGADDRRKMIFSLSHEGRRKIAELHDH
                     RPALPLLP"
     gene            2533..3789
                     /locus_tag="PKH10_05920"
     CDS             2533..3789
                     /locus_tag="PKH10_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT27698.1"
                     /translation="MAEEKEKKVPGPEEDRAPAEPAETTGTTDPSDQPEGEGTGSDTP
                     VPTEEGIPTSPEEAGSPSGTQALPTTDPSAPPQDIFEAIRRQDKEAVVEFLNKKVNLS
                     LINEEDKTPLLCAAEIGALEIVRLLIDHGAALEAKAKDRRTPLMLAISNAHPAVAAYL
                     LQKGADVNVHSITHYTPIMQAAAKGYLDLMREMIPKADKINTQNDEGRTALMIAARFG
                     HRDIIDLLLARNASLQIADKNGNGAISFAETAELKAYLTEKLEEEKRRGETEKEEKKR
                     KKREAHRKVPRPQLLLLALLAFLVMGFGSFSYFYYKTKIKNAIVEIPPFVREAAGIVS
                     HGYCKKTVECRNRKESFMTLCKEQVREFFAAQLVEQGTDLCDNTKAAQCSDCLATLPC
                     NKATQLSPELLKKECLLCLKVCARKD"
     gene            complement(3786..4214)
                     /locus_tag="PKH10_05925"
     CDS             complement(3786..4214)
                     /locus_tag="PKH10_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT27699.1"
                     /translation="MRTARGATLIEMMIVLVILGILSSTAMPMYADYASRSRTSEVPL
                     SLKKIIEAQITFKETNETATFASSIASLGWSTSGDDPATPDLAEGSFYIFFADTMDTC
                     NMGAMPLEGLALAVVRAGVSVPSAWEGVCMDEKMSIYLLP"
     gene            4360..5343
                     /gene="galE"
                     /locus_tag="PKH10_05930"
     CDS             4360..5343
                     /gene="galE"
                     /locus_tag="PKH10_05930"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNT27700.1"
                     /translation="MRILVTGGAGYIGSHLVKLLGEKHKHTITVLDNLSTGHRPAVLY
                     GDLVVTDLAETKAVEDLLTHGRFDAIIHFAAHIVVPESVADPLKYYTNNTLNTMNLIR
                     LCVKHGIGKFIFSSTAAVYGEPDDGVVSETSLLSPINPYGMSKLMSERILLDTARAHP
                     DRFRAVILRYFNVAGADPDGRIGQSFPDATHLIKVAAQAALGKRSQVNIFGTDFPTPD
                     GTGIRDYIHVTDLAHLHIRALDHLASGGDTDIFNCGYGHGYSVREVLGTMKRVSGADL
                     RVIEAPRRAGDPAKLTADNTKVRRAWGWEPRYDSLELICRTALDWERDRTY"
     gene            complement(5337..>5563)
                     /locus_tag="PKH10_05935"
     CDS             complement(5337..>5563)
                     /locus_tag="PKH10_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27701.1"
                     /translation="VSPGELAYYARRIDVLCITSREESMPVVVWEFKGVCPILSFAVG
                     DIAAHLPPEAILKDDDDFVLLLSEMGGRVQ"
BASE COUNT         1237 a         1736 c         1496 g         1094 t
ORIGIN      
        1 acccttcacc atcacgcggc cggcaacggc aaggttgcgg tcgagccagg tcgccacgat
       61 ggggccgccg tatatctcgg tcgcgacgcg gacgacattc ttgcccatgc tctcggcttc
      121 gggctttatc ttgaggagcg ggctgtcggt gtgcgccccg gccatcatga agccgctttc
      181 ccacgggctt ttacggccga tacggaacgc gatgaccgcc gaatcgttgc ggacaacgaa
      241 gtagcccgcg ccgctcttca gcgaccactc gtcgccttct tcgagtcttg agaaccccgc
      301 cttttcaaga cggccgatga tcgaaacggt cgcgtggaac atggtcgggg atccgtcgag
      361 aaaaaggaac aggtcatcca gcgaagcacg catcgttctg tgcatgggtc ctccttttaa
      421 cttatgggtg actgtcggtc cttttagcgc tcgccgccgc ttttttctgt tccatacccg
      481 ttatctcgac gggggtcgtt cgggtctcat cggtgccatc gcccagttgt ccgaactgat
      541 tgcgccccca acagtagaga tatcctcttt tcttgtgttc gcggatggcg caggtatggg
      601 tctctccggc cgacatctgt tgccaatcgg catggtcgct tttcactttt tgcggggcgg
      661 ccacgcgatc cagcctgccc agccccgatt gcccgtaacg gttatcgccc cagcaataga
      721 tcgctcggcc ttcaccctcc tgacggatag cgcaggtgtg gttggcgccg gcggtcagtg
      781 aaagccacga atcgttatcg ccggcgatag gggtcggcgt cgttctcttg gcggtatcgc
      841 cggtcccgag ttgccccgac ccggcgcttc cccagcagta tatcctccgt acgccctgct
      901 cgttgcggat gccgcaggtg tggtagtagc ccgcggagac cgattcccaa tcgacatggc
      961 cgccgccgac ctcgaccggc tcagcgcgtc tcttttcggt cgatccgtca ccgagttgcc
     1021 cgtcaccgtt ggcgccccag cagtagaccc gattctcgcc cccatcctcc ctaagcccgc
     1081 aggtatggga aaatcccccc gacacataac gccacccccg caggtcaccc gccaccggac
     1141 gcggagagag ggcccgctgg taggaggggt cgccgacctg tccggcgccg ttctctcccc
     1201 agcagtaaag cgttcccttc ccgccatcgg tccggatgcc gcaggtgtgc gagataccgg
     1261 cggtcatctg ctcccagtca tcatacccac ccgccacctc catcggcacg aagccggacc
     1321 gctcgtcacc aacccccaac tggccgctcc cgttactccc ccagcagtac acgcgctgat
     1381 ccgaccggtc cttaccaccg ataccacagg tgtgaaaacg accggcgctc acttttcgcc
     1441 accaggcaat gagattccgc ggttcgaccg gataaaaact ctcctgccga tcggagatac
     1501 cgagttgctg ggcgttattg ttgccccagc aatggggttt acccccggca ttcaccgcgc
     1561 aagtatgctg atatcccgtc gagacatgca cgaactgata cggtatctcc cattcaaaaa
     1621 cggtcggttt cgcctcgaca ttccccgccc ggtcgatggc gcgcaccgtg aaggtgtgcg
     1681 tgccctcgga aagggcctcg tagcgctctt ccccggtaca gcgatgccac agttcatcat
     1741 caagtcggca ttcatagcgc gacacgggtt ccgaggcggt gaacctgaaa agtgccgagc
     1801 gctcaagtgt cggggactcc ggtttttcgg tgatcgtcgt gaccggcgac accgtatcct
     1861 tttcctcaac gaccccgtcg ttctcgcccg caagaggaag agaactcgca agcgtcgccc
     1921 aaagaataag tcgccatacg caggaacgac ccattaaata cacaccaccc tgtatgaacg
     1981 atccgggggc actgtagcat aaatcatttt tttttcaaac agatcgggcg gaaatctctt
     2041 gacaacctca ctgcaggcca ttaccgtgcg ttggtaaggt gccggtctgt tcaatgcggc
     2101 ggagggatca tgcgggaact atgcaggctc aaggagatat tccgggccat ctatgttttc
     2161 gagacgacgc tcaagaaacg gttccgtctt tcgctcaacg aagcactcgc tctttgtgcg
     2221 ctggaacagg gtgaaaagaa cagccgcgaa ctcgcgcagg agatcggcat ctcattttcc
     2281 cgtatgtccc gcgttctcgg ttcgctggag gagaaagggc tcattaaaag gaccttcggc
     2341 gccgatgacc ggcgcaagat gatattctct ctttcccatg agggtcgacg gaagatagcc
     2401 gagctgcacg accatcgacc ggcacttccc ctactgccct gacccgcacg gaaaaatagg
     2461 caacgatttt ctagatttcg ggcataactg ttgctataat gcaccccagc attcaacttt
     2521 tcgggggcga gaatggcaga ggaaaaagaa aagaaggtac ccggaccgga agaggatcga
     2581 gcgcccgcgg agccagcgga gacgaccggg acaacggatc cctcggatca acctgaaggc
     2641 gagggaacgg gatcggacac tccggtcccg acggaagagg gtatccccac ctcaccggaa
     2701 gaggccggat cgccatcggg cacccaggcg ctccccacca ccgatccctc agcacctccg
     2761 caggatatct tcgaggcgat acgccgtcag gacaaagagg cggtcgttga attcctgaac
     2821 aagaaggtca atctctccct catcaacgaa gaggacaaga ccccgctgtt gtgcgccgcc
     2881 gagataggtg cgctcgagat cgtccggctc ctcatcgatc acggcgccgc gctggaggca
     2941 aaggcgaaag acaggcgcac cccgctcatg ctggccatca gcaacgcgca tcccgcagtg
     3001 gctgcatacc tcctccagaa aggggccgat gtcaatgtcc actcgataac gcactacacg
     3061 cctatcatgc aggcggcggc caagggctat ctcgacctca tgcgcgagat gatccccaag
     3121 gcggataaga taaacacgca gaatgacgaa gggcgcaccg cccttatgat cgcggcccgg
     3181 ttcggacatc gcgacatcat cgatcttctc cttgcccgca acgcctcttt gcagatagcc
     3241 gacaagaacg gcaacggtgc gatatccttc gccgagaccg ccgaactgaa ggcgtacctc
     3301 accgagaaac tcgaagagga gaaacgacgc ggcgagaccg aaaaggaaga gaagaaacgc
     3361 aaaaagaggg aggcgcaccg caaggtaccg cgcccccaac tgctcttgct ggctttactg
     3421 gcgtttctgg tcatgggttt cggctcgttc tcttactttt attacaaaac aaagatcaag
     3481 aacgccatcg tcgagatacc gccattcgtg agagaggcgg ccggtatcgt ttcacacggt
     3541 tactgcaaga aaacggtcga atgtcgcaac cgcaaagaaa gtttcatgac cctctgtaaa
     3601 gaacaggtca gagaattctt tgccgctcaa cttgttgaac aaggaactga tttgtgcgat
     3661 aacaccaagg cggcgcagtg tagcgactgc ctcgcgaccc tcccctgcaa caaggcaacg
     3721 caactgtcac ccgaactgct gaaaaaagaa tgccttttgt gtctgaaggt ttgcgcgcga
     3781 aaggactaag gcaacaagta gatagacatt ttctcgtcca tacagacacc ttcccatgcg
     3841 ctggggactg aaactccagc tcggacgacc gcaagggcca acccttctag gggcatcgcc
     3901 cccatgttac aggtgtccat cgtatcggcg aagaagatat agaaggaacc ctccgcgaga
     3961 tcgggggtcg cggggtcatc tccgctggta ctccacccca gagaggcgat actactggca
     4021 aaggtcgcag tctcattggt ttccttaaag gtgatctgcg cctcgatgat ctttttcagg
     4081 gaaagaggaa cctctgaagt gcgggaacga gaggcataat cggcatacat gggcatcgcc
     4141 gtcgaggaaa gtataccaag gatgaccaag acgatcatca tctctatcag ggtagcaccc
     4201 cgtgccgtgc gcatgactct cctccctaaa aaaacaacca tccggttgga tgatagcaaa
     4261 aaaaagaccg aagtcatcaa aaaatctgat atttcttgtt gtcttaaaca tcttttggcg
     4321 tatattgggg tagttatcgt catgttcgag gtggtatcca tgcgtatact cgtgaccggc
     4381 ggcgccggat acatcggcag ccatcttgtt aaattgcttg gggaaaaaca caagcatacc
     4441 atcaccgtcc ttgacaacct ctctaccggc caccgccccg cggtcctcta tggcgacctc
     4501 gtggtaacgg accttgccga gacaaaggcg gtcgaagatc ttttgacgca cggtcgtttc
     4561 gatgccatca tccatttcgc cgcccatatc gttgtacccg aatctgtcgc cgatcctctg
     4621 aaatactaca cgaacaacac tctcaacacc atgaacctga tacggctctg cgtgaaacac
     4681 gggatcggta agttcatctt ctcttcgact gcggcggtct acggtgaacc ggacgacggc
     4741 gtggtctcgg aaacgagcct tctctctccc atcaacccct acggcatgag caaactgatg
     4801 agcgagcgga tactcctcga taccgcccga gcccaccccg accgcttccg ggcggtcatc
     4861 ctgcgttact tcaatgtggc cggcgccgac cccgacggca ggatcggtca gagttttccc
     4921 gatgccaccc acctcatcaa ggtggcggcg caggcggctc tcggcaaacg atcgcaggtc
     4981 aacatattcg gcaccgattt ccccacccct gatggcactg gcattcgcga ttacatccat
     5041 gtgaccgacc tcgctcacct tcatatccgg gcgctcgacc accttgcgtc gggaggcgac
     5101 accgacatct tcaactgcgg ttacggccat ggatacagcg ttcgggaggt gctgggcacc
     5161 atgaaaaggg tcagcggcgc cgatctccgg gtgatcgagg caccccgccg cgccggtgat
     5221 ccggcgaaac tcaccgccga caacaccaag gtgcgccgcg catggggatg ggaaccccgc
     5281 tacgactcgc tggaactcat ctgccgcacc gcactcgatt gggaacggga ccggacctac
     5341 tgaacccgtc cccccatttc cgacagcaac agaacgaagt catcatcatc tttcaatatc
     5401 gcttcgggcg gcaggtgagc ggcgatatcg ccgacggcga aggaaagtat cggacaaacc
     5461 cctttgaatt cccagaccac caccggcatg ctctcttccc gcgaggtgat gcacagcaca
     5521 tcgatccgac gggcataata ggcaagttcc ccggggctca cca
//
LOCUS       DAOQUE010000151         5504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16286_length_5504_cov_4.467976, whole genome shotgun sequence.
ACCESSION   DAOQUE010000151 DAOQUE010000000
VERSION     DAOQUE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16286_length_5504_cov_4.467976"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1731)
                     /locus_tag="PKH10_05940"
     CDS             complement(<1..1731)
                     /locus_tag="PKH10_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNT27702.1"
                     /translation="MDWKTIAFEVFGGLGLFLLGMKIMSEGMQKVAGDRLRKVLGFLT
                     QNRFMAILVGMTVTAVIQSSSATTVMTVGFVNAQLRTLTQAIGVILGANIGTTVTGWL
                     VSLAVVKYAMVIIGIGVLLRFAGHNETVRYSGEAISGLGLLFLGMTTMSDGLAPLRDS
                     PGFVHFFTMVDGVTYPSILLGDCRGTVTNIAVQSSSATIGIAIALATQGLLTITGAVS
                     LILGDNIGTTITALLASIGTNHHAKRAAFAHTMFNVFGVVIILIIFFPFVALVNTFIP
                     QDADLTIRTAEEAARYGMAIGAKPFIGPHVAMAHTMFNVTNVIFFTPLIGFLAFMVRK
                     IVPEPKAKPSKGVRQFVHIHYGLVETPALGILESEKELYAMAERVKKNSKRIRDILGG
                     KREAGTAAEKIEKNEELIDEYRMAITEFLLSLAQRSLSREDAAKVGNYITCAHNLEKY
                     ADYVTNMTRAYLKMKSDNLILSDAARTSLYDIVSDIEEFYEATIVPLSGVEVDAKNFL
                     ATATTRKREIKERVRAAKMGHFERLQAGTCKGEASMAYVDILDNIDGMVSQVHNIAET
                     LTLSKFSGTSA"
     gene            complement(1854..3584)
                     /locus_tag="PKH10_05945"
     CDS             complement(1854..3584)
                     /locus_tag="PKH10_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNT27703.1"
                     /translation="MDWKTVAFEVCGGLGLFLMGMHILSEGMQKVSGDRLRRILGFLT
                     QNRFMAIFVGLSITSVIQSSSATTVMAVGFVNASLLSLEQAIGVILGANIGTTITGWL
                     VSLSIVQYAMVIIAAGVLLKFIGRNETVRYSGEAIFGLGILFLGMNLMSDGLAPLRDD
                     KEFVSFFTKVDGRTYSSVFIGVLIGTVATALVQSSSATLGIIIALASQGLLSFPAAIA
                     LVLGTNIGTTVTALLASIGTNYHAKRAAAAHILFNFFGVIIVLIVFYPFLRMVDLFLP
                     GDPDMVIRTAEEAAKYGFGIGAKPLAGVHIALAHSLFNITNVVIFTPLIAFLAFVVKK
                     IVPEPKAKLPKTPHQFAHIHYGLIATPALGIIESEKELYAMAERVKKNSKRIRDILGG
                     KREAGTAAEKIEKNEELIDEYRMAITEFLLSLAQRSLSREDAAKVGNYITCAHNLEKY
                     ADYVTNMTRVYLKMKSDNLVLSDAARTSLQDIVNDIEEFYESTIVPLSGVEVDAKNFL
                     ATATMRKKEIKDRIRAAKMGHFQRLQEGTCRGEASMSYIDILVNVDGMVSQVHNIAET
                     LTLSKFSGIA"
     gene            complement(3695..4396)
                     /locus_tag="PKH10_05950"
     CDS             complement(3695..4396)
                     /locus_tag="PKH10_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27704.1"
                     /translation="MRVLLVIVVLTCLALPLCAQVPEELPDTPTLVSKLRAKYAPIYK
                     KYTGFETTRDFTTKTYDPETGELLSTARTVVRKKEYFYKEPDSTVISHEENGKKLDPG
                     DYDTTEIEPFWPLFDDKSAEHYDFRVSGTATIEGRPCIMLEVTPKGNDYRHFKGNAYI
                     TTDTVELLMIEGRPTKTHWAMKEFSVKYLYEPVSGGYCMKKGWFTARVYVFLVRPDRR
                     HVYEVTGRETKPLVK"
     gene            4530..>5504
                     /locus_tag="PKH10_05955"
     CDS             4530..>5504
                     /locus_tag="PKH10_05955"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HNT27705.1"
                     /translation="MRFFYLMILVVGFIGACDGGAVNGEKDDKDVTDIEDGTFDQDIV
                     EVEDDLLPDDDGPVPQALEGPVITLNDNDKAPLAAVGTLTTDIPAVVRVTITAGDESR
                     VAEVLGEPAVEHRFPVLGLFPGVKNSIAVAVRAPDGPWATVGDPVTLDAPALPDDLPP
                     IDITVNTPDRREQGWTLLNISHSTGTALVDFTYGCLIVALDREGRVGWYFRDGGDCYD
                     FGRFSDGRMFVQFTKRIIALDMLGTVSAAWHPVGEAAPYTFSVPIAVDDFHHVVTELP
                     SGNIAILANERRDIADWPTSETDPDAPLADTGIRADEIVELTKEGTEVG"
BASE COUNT         1097 a         1499 c         1735 g         1173 t
ORIGIN      
        1 cgcggaggtt cccgagaact tcgaaagcgt gagcgtctcg gcgatgttgt ggacctgtga
       61 gaccatgccg tcgatgttgt cgaggatatc gacataggcc atcgaggcct ctcccttgca
      121 ggtgcctgcc tgcagccgct cgaaatgtcc catcttggcg gcacgcaccc gttcctttat
      181 ctccctcttg cgcgtcgtgg cggtggcgag gaaattcttc gcgtcgacct cgacgccgct
      241 gagcgggacg atggtcgctt cgtagaactc ctcgatgtcg ctcacgatat cgtacagaga
      301 ggtgcgcgcc gcgtcggaaa ggataaggtt gtcactcttc atcttgaggt aggcgcgggt
      361 catgttggtg acatagtcgg cgtatttttc gaggttgtga gcgcaggtga tgtaattgcc
      421 gaccttcgcc gcgtcttcgc gggacagcga acgttgcgcc agcgacagga ggaactcggt
      481 gatcgccatg cggtattcgt cgatgagttc ttcgttcttc tctatcttct cggccgctgt
      541 gcccgcttcc cgttttccgc cgaggatatc gcggatgcgc ttcgagttct tcttgacccg
      601 ttcggccatg gcatagagtt ccttttccga ctcaaggatg cccaaagcag gtgtttcgac
      661 cagcccgtaa tggatatgaa cgaactggcg gacccccttt gacggtttcg ccttcggctc
      721 gggcactatc ttcctgacca taaaggcgag gaagccgata agcggcgtga agaagatgac
      781 gttggtgaca ttgaacatgg tgtgggccat ggctacatgg ggcccgatga agggcttcgc
      841 gccgatcgcc atgccgtagc gcgccgcctc ctcggcggtc cgtatcgtca gatcggcgtc
      901 ctgcgggatg aaggtgttca cgagcgcgac gaagggaaag aaaatgatca ggatgatgac
      961 aacgccgaac acattgaaca tcgtatgggc gaaggcggcc cgtttggcgt ggtgattggt
     1021 cccgatgctc gccaacagcg ccgtgatggt ggtgccgatg ttgtcgccca atatcagtga
     1081 gaccgcgccg gtaatagtga gcaggccctg cgtggcgagc gcgattgcga taccgatggt
     1141 ggccgaactc gactggactg cgatgttggt caccgtgccg cggcagtcac cgagaaggat
     1201 actggggtag gtcaccccgt cgaccatggt gaagaaatgg acgaacccgg ggctgtcgcg
     1261 caaaggggcg agtccgtcgc tcatcgtggt catgccgaga aagagcaggc cgagtcccga
     1321 tatcgcttcg ccactgtagc gaacagtctc gttgtgaccg gcgaagcgca acaggacgcc
     1381 gataccgatg atgaccatcg cgtatttgac caccgcgagc gacacgagcc agccggtgac
     1441 cgtggtgccg atgttggcgc cgagtatgac gccgatcgcc tgcgtgaggg tcctgagttg
     1501 cgcgttgacg aacccgacgg tcatgacggt ggtggccgaa ctcgactgga tgaccgccgt
     1561 gaccgtcatg ccgaccaata tcgccatgaa gcgattctgt gtcaggaagc caagtacctt
     1621 gcggagccgg tcgcccgcga ccttctgcat gccttccgac atgatcttca tgccaagaag
     1681 gaacagacca agtccgccga agacctcgaa cgctatcgtt ttccagtcca catgtctctc
     1741 cactcttctg tcagataagg gtcagcgttc cgttgataca tgtttaagga aggattagca
     1801 actaaatgga gggaggggat tgttaaaaaa cggttaattt ctcgctccgg tattcaggcg
     1861 atacccgaga atttggagag cgtgagcgtt tcggcgatat tgtggacctg cgagaccatg
     1921 ccgtcgacat tgacgaggat atcgatatag gacatagaag cctctcctct acaggttcct
     1981 tcctgaagcc gctggaaatg gcccatcttc gcggcgcgga tacgatcctt tatctccttt
     2041 ttacgcatcg ttgccgtagc gaggaaattt tttgcgtcaa cctcgacccc gctcaacggg
     2101 actatggtcg attcgtagaa ctcctcgata tcatttacga tatcttgcag agaggtgcgc
     2161 gctgcatcgg agaggacgag gttgtcactc ttcatcttca ggtaaacgcg ggtcatgttg
     2221 gtgacatagt cggcgtattt ttcgaggttg tgagcgcagg tgatgtaatt gccgaccttc
     2281 gccgcgtctt cgcgggacag cgaacgttgc gccagcgaca ggaggaactc ggtgatcgcc
     2341 atgcggtatt cgtcgatgag ttcttcgttc ttctctatct tctcggccgc tgtgcccgct
     2401 tcccgttttc cgccgaggat atcgcggatg cgcttcgagt tcttcttgac ccgttcggcc
     2461 atggcataga gttccttttc cgactctatg atacccagcg cgggggtcgc gatgagaccg
     2521 tagtggatgt gggcgaactg atgcggcgtc ttgggcagtt tcgccttcgg ttcaggaact
     2581 atcttcttca cgacgaaggc gaggaaggcg atgagcgggg tgaagatgac gacattggtg
     2641 atgttgaaca gcgaatgggc gagcgcgata tggacgccgg ccagtggctt ggcgccgatg
     2701 ccgaaaccgt acttcgccgc ctcttcagcg gtccgtatca ccatgtcggg gtcgcccggc
     2761 aggaagaggt cgaccatgcg aaggaagggg tagaagacga tgaggacgat gatgaccccg
     2821 aagaaattga acaagatatg cgcggcggcg gcccgtttag cgtggtaatt ggtcccgatg
     2881 ctcgctaaca gtgccgtgac ggtggtgccg atattggtgc cgagcaccag cgcgatggct
     2941 gccgggaagg aaagcagccc ctgtgaggcc agcgcgatga tgatgccgag cgttgccgaa
     3001 ctcgactgga caagggccgt cgccaccgtg ccgatgagaa ccccgatgaa tacgctggag
     3061 taggtgcggc cgtcgacctt ggtgaagaag gaaacgaact ccttgtcatc gcgcagaggg
     3121 gccagaccgt cgctcatgag gttcatcccg aggaacagga tgccgagtcc gaatatcgcc
     3181 tcgccgctgt agcgcaccgt ttcgtttcgc ccgatgaact tcagaaggac gccggcggcg
     3241 atgatgacca tcgcgtactg tactatcgac agcgacacga gccagccggt gatggtggtg
     3301 ccgatgttgg cgccgaggat gacgccgatc gcctgttcga gcgacagcag gctcgcgttg
     3361 acgaagccga ccgccatgac ggtggtggcc gaactcgact ggatgaccga agtgatggaa
     3421 agcccgacga agatcgccat aaagcgattt tgtgtgagga agccgagtat cctgcggaga
     3481 cggtcgcccg agaccttctg catgccctcc gacaggatat gcatgcccat gaggaacagg
     3541 ccgagaccgc cgcagacctc gaacgctacc gttttccagt ccataccccg cctcttttgt
     3601 gaagagcaag tttgttttcc gttaataccc ggttaagaaa cgattagcaa taaaagcggc
     3661 tcggaaaggg gagaaaaatc ttaggtgcct gatatcactt gactaggggt tttgtctcgc
     3721 gaccggtcac ctcgtagaca tgacggcggt cggggcggac gaggaacaca tagacgcggg
     3781 cggtgaacca gcccttcttc atgcagtagc caccggaaac cggctcgtag aggtatttca
     3841 ccgagaactc cttcatcgcc cagtgcgttt tggtcggtct tccctctatc atcaggagtt
     3901 cgacggtgtc ggtcgtgatg taggcgttac ccttgaagtg gcggtagtcg ttccctttcg
     3961 gggtcacttc gagcatgatg caggggcgcc cctcgatggt ggcggtcccc gagacgcgga
     4021 agtcgtagtg ctccgcgctc ttgtcgtcga aaagcggcca gaacggctct atctcagtgg
     4081 tgtcgtagtc gcccggatca agtttcttgc cgttctcctc gtgactgatg acggtgctgt
     4141 cgggttcttt gtagaaatat tcctttttcc gcacgacggt gcgggcggtc gagaggagtt
     4201 cgcccgtctc gggatcgtag gtcttggtcg tgaaatcgcg ggtggtctcg aacccagtgt
     4261 attttttgta aatgggggcg tatttcgccc gcagtttcga gacgagcgtt ggggtgtcgg
     4321 gcagttcttc ggggacctgt gcgcagagcg gcagggcgag gcaggtgagg acgacgatga
     4381 cgagcaggac gcgcaaaagg acactccgca aaaaaaccgt cgcgattata gcggctctgt
     4441 tgcgggaggc aagaaaagaa gagcccgggt gattgacaaa aacatcgttc ggtcttatcc
     4501 tgcctctgtt ctgatgagcg gagagccaga tgcgcttttt ttatctcatg atccttgtcg
     4561 tcggtttcat cggtgcctgc gacggcggcg ctgtcaacgg ggaaaaggac gacaaggacg
     4621 taaccgacat agaggacggg acctttgatc aagatatcgt cgaggtggag gacgacctgc
     4681 tccccgatga cgatgggccg gtaccgcagg cgctcgaagg tccggtgata acactgaacg
     4741 acaatgataa ggcgcctctc gcggcggtcg gcacactcac gaccgatatt ccggccgtgg
     4801 tccgcgtcac gataacggcg ggcgacgaga gccgcgttgc cgaggtgctg ggcgaaccgg
     4861 cggtcgagca ccggttcccc gtactggggc tcttcccggg cgtcaagaac agcatcgccg
     4921 tcgcggtacg cgcccctgac gggccgtggg ccaccgtcgg agacccggtc acgctcgatg
     4981 ctccggcgct tcccgatgat cttccgccga tcgatatcac cgtgaacacg ccggatcgtc
     5041 gcgagcaggg atggacgctt ctcaacatct ctcactcgac aggcaccgcg ctggtcgatt
     5101 tcacctacgg atgcctcatc gtcgctcttg atagggaggg gcgggtcggc tggtactttc
     5161 gtgacggcgg tgattgttac gatttcggcc ggttcagcga tggtcgcatg ttcgtccagt
     5221 tcaccaagcg gatcatcgcg ctcgacatgc tcggcacggt gagcgccgcg tggcacccgg
     5281 tcggcgaggc cgcgccctac accttcagcg tgccgatagc ggtggacgat tttcatcatg
     5341 tggtgaccga actgccttcg gggaacatcg cgatactcgc gaacgaaagg cgggatatcg
     5401 ccgactggcc cacgagcgag accgatcccg atgcaccgct cgccgatacc ggcatacggg
     5461 ccgacgagat cgtcgagttg accaaggagg ggaccgaggt cggg
//
LOCUS       DAOQUE010000152         5493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16339_length_5493_cov_4.453292, whole genome shotgun sequence.
ACCESSION   DAOQUE010000152 DAOQUE010000000
VERSION     DAOQUE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16339_length_5493_cov_4.453292"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1580
                     /locus_tag="PKH10_05960"
     CDS             147..1580
                     /locus_tag="PKH10_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27706.1"
                     /translation="MMPIRVGLFCSFLLFVFAGASLFAQEGENEKQAEKGFRLSDTAV
                     MNVHFTLAPEFESNINNVSTRSYYEDHRDSANNYTHAETKDLVLHLSPGIRAKIDDKF
                     KTLAMAFLINYNLYTGLDDKKSFEKLTTLSALHFDTDILGEFNKDGNVVVRALNNLKR
                     NSNLDVLTIENGLHTNLLEDFSLSVYVKNSEETLSLKLSSGIQLNYFEESLFSPYNFW
                     SSRSNAYGQWKFLPKTAAFFNASFTYQDYFNADPVSRHDQRSMPLSVFVGVLGQITPK
                     ISLKLLGGYANSFSKEMHHDGLAGAEFVYKHSENTLFNLGYLRSLSPVAVYQYYSMDK
                     AYLGFTQKLLHSRLLLKFDASYSHLAYGKNVKYPLENNPAQTRVDDNDTPNDASDDIT
                     TVTTVTQKSVDREDHLIRIEPMVSYNILHWLGISLKYSFETKQTPFYATTRITRSGGG
                     VPPSDVSYHLYYDYMDHRVLLSLAADY"
     gene            1588..2253
                     /locus_tag="PKH10_05965"
     CDS             1588..2253
                     /locus_tag="PKH10_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNT27707.1"
                     /translation="MRLASRGLLIIIIATAMTATMGCTLKFARDEAASPAARSSSSVM
                     AERVPDEVDAASLQPGIIGAGDLLQITVYGEKDLTNLYQVSPEGVILFPFIGEITAKG
                     MSNFSLAERIAEKLKDGYVKNPQVHILVKEFNSRRLFVLGQVKNAGKFSIVHDMSIIE
                     AIALAGGFTNLADIDNVVVTRRDETGKEVRYVLNVDAIVSGKTNNFYLLPGDIVFVRE
                     RFF"
     gene            2261..4405
                     /locus_tag="PKH10_05970"
     CDS             2261..4405
                     /locus_tag="PKH10_05970"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNT27708.1"
                     /translation="MEARDLDIKQLAILLLKHRYLFIAVIVVVLTVTAAYLYKAPRIY
                     RATAVIEIGPRSENILGRRVESITSSGADYYWANKEYAATQYEIIKSRAVSSKVIESL
                     SQENVLDLLGIRQDEDASAEDIKNIDTVSILQGKISVDPQKNSNIVRIGLSDTDPEVA
                     AFLANSVANSYIEFNIEKNYLATRDAAKWLSEQSINLKKQLEESEITLFNFKKENEVL
                     ASAFEQRQAMLAQTITEISSKLTEKQIYQKALNAKIEELSSLEFADTEEFFTLDLFRD
                     KPVIQNMKLQYLEIRRKLREKAILYGDQHPDISALRSDEKNIRAALLGELKGILEGYR
                     VEGKTLDNEIRKLQGMLQESQKEALKLNKLEIDYNKLKREVETNKKLYDIVLERTKEA
                     DLHTLMRANNIRVIDRALVPKGPVSPRKNLVLLVGLIIAVLSALTAVTAAEFFDTRLR
                     DVEDLERIVGRNILGIFPSFDIEDPGKIPEIAFEGSQHSPAVESLRTIRTNIRLAHPD
                     VNAKSILVTSSLSQEGKTTVSANIAVSFALAGRRTLLVDTDMRKPRVHKLFGIDNKEG
                     ISTHMLGEKGIEQVIRRDVYQGLDVLLCGPIPPNPAEMMESKRFRDMTERLRDLYDII
                     IFDSPPIIAVSDAAILSSMIDGVVVVVKIRQVSRDILKRSVSQLTKSNALLLGAVVNN
                     LDLKGGGRYGSYYYYYHEKYRYYGEAQEKKPA"
     gene            4402..5049
                     /locus_tag="PKH10_05975"
     CDS             4402..5049
                     /locus_tag="PKH10_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNT27709.1"
                     /translation="MNHAAFGIPLLLCLVAISCGPRLHVRHPVAPGDTYFSWVTWYGD
                     DFKGKKTAGGEPYDPLSFTCAADGFPFDTLLEVRDLDGGGAVVVRVTDRPGKNVVDLT
                     PKAFRLLSAEQAGRLRGRVTVVGLPGENSLLRRNETGAFQTFFTVQVGAYSTLENARA
                     FIDSLGIAEAYIYLEQGDRSLYRVRAGRFADRESAENFQKERMRGHESVVVEVSE"
     gene            5060..>5493
                     /locus_tag="PKH10_05980"
     CDS             5060..>5493
                     /locus_tag="PKH10_05980"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNT27710.1"
                     /translation="MLFPLFLFADDVLSQILRSEATVTSFDPTLRIGIVQSVDTLNID
                     GDAQMSDTDGRALGAIQGACRIVPVATGKISVCDRTYPAPVTFTPKFGFMSVAGKMFR
                     GKVEVVLSEKGLTAVNALHIEEYVRGVINKEIMPGWHIEAKKA"
BASE COUNT         1275 a         1499 c         1521 g         1198 t
ORIGIN      
        1 gacggcgccg gcaccgacac ccccggcgga ccgactggga cgcccgcgaa caccggggga
       61 gatacggggg cgcccacgga taccatgcct gtgttcatag agccgagtcc cgagaaatga
      121 agtagatatc cggtgagatc ttatttatga tgcccatacg cgttggtctc ttttgttcct
      181 tccttttgtt cgtcttcgcc ggcgcttctt tgttcgcgca ggaaggggaa aatgaaaagc
      241 aggccgaaaa gggattccgc ctttccgata cggccgttat gaatgttcac tttacgttag
      301 ccccggagtt cgaatcgaac atcaataatg tttcaacacg ctcctattac gaggatcatc
      361 gtgactcggc gaataactat acccacgcgg agaccaagga tcttgtcctg catctttccc
      421 cgggtatacg cgcaaagata gatgacaaat tcaaaaccct ggcaatggca ttcctcatca
      481 actacaacct gtacacgggt cttgatgaca agaaaagttt cgagaaactc acgacacttt
      541 cagcgctcca tttcgatacc gatatcctcg gcgagttcaa caaggacggc aatgtcgtcg
      601 tccgtgcgct caacaacctc aagcggaatt ccaatctcga tgtgttgacc atagaaaatg
      661 gacttcatac gaacctcctg gaggatttct cactgtccgt atatgttaag aacagcgagg
      721 aaacgctctc cttgaaattg tcctccggca tccaactcaa ttatttcgag gagtcgctgt
      781 tcagccccta taatttctgg agttctcgga gcaatgccta cgggcaatgg aagttcctgc
      841 cgaaaacggc ggcctttttc aatgcctctt tcacctatca ggattatttc aatgccgatc
      901 ccgtttcgcg tcatgaccag cgttcgatgc cgctgtcggt ttttgtcggc gtgcttggcc
      961 agatcacccc taagatatcg ctgaaacttc tgggagggta cgcgaattcg ttctccaagg
     1021 agatgcacca cgacggtctg gcgggggcgg agttcgtgta caagcattcc gagaacacgc
     1081 tcttcaacct tggttacctg cgctcccttt ctccggtggc ggtctatcag tactactcga
     1141 tggataaggc ctatctggga ttcacgcaga aattgctgca tagccgtctt ctcctcaaat
     1201 tcgatgcgag ttattcgcac cttgcctatg gcaagaatgt caaatatccg cttgagaaca
     1261 atccggctca aacacgggtc gacgataacg atacacccaa cgatgcatca gacgatatca
     1321 ctacggtcac tacggtcacc cagaaaagcg tcgaccgcga ggatcatctc atccgtatcg
     1381 aaccgatggt gtcttataac atccttcact ggcttggtat ctcgctcaag tattcctttg
     1441 aaacgaagca gaccccattc tatgctacga cgaggatcac gcgcagtggg ggcggtgtgc
     1501 cgcccagtga tgtttcctac catctttact acgattacat ggaccatcgc gttctgctga
     1561 gtctggcggc ggattattga tgtgatcatg cggctcgctt cacgaggcct cctcatcatt
     1621 attatcgcaa cagcgatgac cgcaacaatg ggctgtaccc ttaaattcgc ccgcgacgaa
     1681 gccgccagcc cggccgcccg atcttcctcc tcagttatgg ccgaacgggt tcccgacgag
     1741 gtggacgcgg cctccctgca gcccggtatc atcggcgccg gcgatctgct ccagataacg
     1801 gtctacggag agaaggatct cacgaacctg tatcaggtga gtcccgaggg ggtcatactt
     1861 tttcccttta tcggcgaaat aacggcgaaa gggatgagca acttctctct ggcggagcgg
     1921 atcgccgaga aactgaagga cggctatgtc aagaatccgc aggtgcacat actggtgaag
     1981 gagttcaaca gccgccgtct gttcgtgctc gggcaggtca agaacgcagg taagttctca
     2041 atagtccacg atatgagcat cattgaagcg atcgcgctcg ccggaggatt caccaacctc
     2101 gctgatatcg ataatgtcgt ggtcacgcgc cgcgacgaaa cgggaaaaga ggtgaggtat
     2161 gtgttgaatg tggacgcgat agtgtctgga aaaacaaata atttttactt gttgccgggc
     2221 gatatcgtat tcgtccggga acgcttcttc taggttgccc atggaagcgc gcgaccttga
     2281 tatcaagcag ctggccatcc tgctcctgaa gcaccgctat ctcttcattg ccgtcatcgt
     2341 ggtcgtgttg accgtcaccg ccgcctatct gtacaaggcg ccgcgcattt accgagcgac
     2401 cgcggtcatc gagatcggcc cgaggtccga gaacatactg gggcgtcgcg tcgaatcgat
     2461 aacctcgtca ggggccgatt actactgggc aaacaaggag tatgcggcga cgcaatacga
     2521 gataatcaaa agccgtgcgg tcagttcgaa ggtgatcgaa agtctctcgc aggagaatgt
     2581 tctggatctt ctcggcatcc gtcaagatga ggatgcttca gccgaggaca tcaagaatat
     2641 cgacacggtc tccatcctgc agggcaagat atcggtcgac ccgcagaaga actcgaacat
     2701 cgtgcggatc gggctctcgg ataccgatcc cgaggtcgcc gccttccttg ccaacagcgt
     2761 agcgaactcg tacatagagt tcaacataga aaagaactat cttgcgaccc gcgatgcggc
     2821 caagtggctt tccgagcaga gtatcaatct caagaaacag ttggaggagt ccgagataac
     2881 gcttttcaac ttcaagaaag agaacgaggt gctggcgtcg gcattcgagc agcgacaggc
     2941 gatgttggct cagacgatca ccgaaatatc gagcaaattg acagagaagc agatatatca
     3001 gaaggcgctc aacgcgaaga tagaggagct ttcctcgctc gagttcgctg ataccgagga
     3061 attcttcacg ctcgatctgt tccgcgacaa gccggtgata cagaacatga aactccagta
     3121 cttggagata cggcgaaaac tgcgcgagaa agcgatcttg tacggcgatc agcatcccga
     3181 tatatccgcg ctccgatcgg acgagaagaa catccgggcg gcgcttttgg gcgaactgaa
     3241 aggtattctt gagggctacc gggtcgaagg aaagacgctg gacaacgaga tacgcaaact
     3301 gcagggtatg ctacaggaaa gccagaaaga ggcgttgaaa ctgaataaac tggaaataga
     3361 ctataacaaa ctgaaacggg aagtggagac caacaagaaa ctctacgata tcgtcctcga
     3421 gcggaccaaa gaggccgacc tgcacacgct catgcgggcg aataacatcc gggtcatcga
     3481 tagggcgctg gtcccgaaag ggccggtgtc gccgcgcaag aatctcgtcc tgctggtcgg
     3541 gctcatcata gcggtccttt cggcgctgac ggccgtgacg gcggcggagt tcttcgacac
     3601 gcgcctgcgc gatgtggagg atcttgagcg tatcgtggga cgcaatatac tcggcatctt
     3661 tcccagtttc gacatagagg atcccggcaa gatacccgag atcgcctttg agggttctca
     3721 acattctccc gcggtcgaat cgctccggac gatacgcacg aatatccggc tcgcccatcc
     3781 cgatgtgaac gcgaaaagca ttcttgtcac cagttcgctc tcacaggaag gtaagacgac
     3841 cgtttccgcc aatatagctg tgagttttgc gcttgccgga cgccggaccc tgctggtgga
     3901 taccgacatg cgcaaaccgc gagtccacaa actcttcggt atcgacaaca aagagggcat
     3961 atcgacccac atgttgggcg aaaagggtat cgagcaggtc atccggcggg atgtctatca
     4021 ggggcttgat gtccttttgt gtggccccat cccgccgaat ccggccgaga tgatggaatc
     4081 aaaaagattc cgcgatatga ccgaacgact ccgcgatctc tatgatatca taatctttga
     4141 ctcgccgccg ataatcgccg tttcagacgc ggccatcctt tcctccatga tagatggcgt
     4201 ggtggtggtg gtcaagatac ggcaggtgtc acgcgacata ctcaagcgct cggtctctca
     4261 gctgaccaaa tcgaacgcac tgctgctcgg agcggtcgtg aacaatctcg atctcaaggg
     4321 cggcggccgg tacggttcct actactatta ttaccacgag aaataccgct actacgggga
     4381 agcgcaggag aaaaaacctg catgaaccat gcggcgttcg gcataccgtt acttctgtgc
     4441 cttgtcgcga tctcctgtgg tccgcggctc catgtgcgtc acccggtggc gcccggcgat
     4501 acctatttca gctgggtcac ttggtatggc gacgatttca agggtaaaaa gaccgccggt
     4561 ggcgagccgt atgacccgct ctcattcact tgtgccgccg atggattccc gttcgacacg
     4621 ctactcgaag tgcgcgacct cgatgggggc ggtgcggtgg tcgtacgggt taccgaccga
     4681 ccgggcaaga atgtggtcga tctgacgcca aaggccttcc gtctgctttc ggcggaacag
     4741 gcggggcgcc tgcgcggtcg agtgacggtg gtgggcctgc cgggcgagaa cagtctgctc
     4801 cgtcgtaacg aaacgggggc gtttcagact ttcttcacgg tgcaggtcgg cgcctattca
     4861 accctggaga acgcgcgcgc tttcatcgat tctctcggca tcgccgaggc ctacatctat
     4921 cttgagcagg gcgaccggtc tctctaccgg gtgcgtgccg gccggttcgc cgaccgtgag
     4981 agcgcggaga acttccagaa ggagcggatg aggggccatg aatcggtcgt cgtcgaggtc
     5041 agcgagtagt ctcgctcttt tgctgttccc gctcttcctt ttcgccgatg atgtcctttc
     5101 gcagatactt cggtctgaag cgaccgttac ttcgttcgac ccgaccctcc gcatcggcat
     5161 cgtgcagagc gtcgatacac tgaatatcga cggggatgcc cagatgtcgg acaccgacgg
     5221 tcgagcactc ggcgcgatcc agggagcatg tcgtatcgtc ccggtcgcaa cggggaagat
     5281 atcggtctgc gacaggacct atccggcgcc ggtgaccttc acgccgaagt tcggtttcat
     5341 gtcggtcgcg gggaagatgt tccggggaaa ggtcgaagtg gtcctttcgg aaaaaggact
     5401 gacggcggtc aacgcactgc atatcgaaga gtatgtccgc ggtgtcatca acaaggagat
     5461 catgccgggg tggcatatcg aggcgaagaa ggc
//
LOCUS       DAOQUE010000153         5482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16367_length_5482_cov_4.500645, whole genome shotgun sequence.
ACCESSION   DAOQUE010000153 DAOQUE010000000
VERSION     DAOQUE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16367_length_5482_cov_4.500645"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PKH10_05985"
     CDS             <1..492
                     /locus_tag="PKH10_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT27711.1"
                     /translation="HAGIAVKGVDTHGLAQRGGIVVSHLRIGDEVYSPLIPEGDTDIA
                     VALERHEALRALNGFVKDGGTLVWYDTVWQPLEVRVGATPEVGRETVAEECERRTVKN
                     VRVRVDDLPDTRMQNIALMAAVCREGLIPGIKKEHYEAAMGDLMEGRMLEKNRALFTR
                     LIG"
     gene            498..2819
                     /locus_tag="PKH10_05990"
     CDS             498..2819
                     /locus_tag="PKH10_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNT27712.1"
                     /translation="MQHEWVQILDMVAEGCFSAAGRRHIHDLAPLSRGEAEREFRLIG
                     ETATLFDTGVAIFNYDLDRVSGVVERFRRDESLEPMDYRVVGDLLEQLRRAKQEVGRQ
                     TWVREFALLFAPFEPDVGLEMTIKQTVDKKGEITSDASPELARIRRHIADTHQLIGRE
                     IKRMIADPAVGGMLQEDYFTIRDDRYVLPFKTQFKRVMNGVVHNLSRSGQTAFLEPLA
                     LVELNNRLSLLASHEQNEILKVLRELRGLLGRRIDHVEACVGLALRLESLYVRQRWMR
                     SYDCAVPEFTDEGATADEVWYPPVLAALKKGTVRNAFRFKEGERIMVISGPNAGGKTV
                     ALKSFHTVVELARRGIPVPAKRARIPFFDEVFLVLGDQQDAAEGESSFSAHLRNLAAI
                     AHRAGERSLVLVDEIGTGTDPQQGGAIARAFLEFLRDRRSFTVVTSHLAEVKAIALED
                     PAFVPVAMGFDEKNERPTYRFMYHLVGGSNALALVRRMDFPPELVRLLENLLSSRDGS
                     IEPLLNRLRQKEQELDEEQERISALKAETVKQKEEIAALERKLAQKERTFEQERMKML
                     KKLMELEEAQLRRRVEETDAKNAAKRIGIVRKEKEALAQAIEREKAKADETPGVPLAE
                     AEGVRVGETVVYDRLTKIKGSLSALKGAKAEVTTGGKSVLVPVERLIVVGEKRKKRAV
                     AAPLDTGAACFEKCDVRGMTVEDACALVEKTVDGAYAAKSIAVAVVHGHGTGALKKGV
                     RSYLEQLAGRYHFVWRSAPTEQGGDGTTLVEFS"
     gene            2910..4601
                     /locus_tag="PKH10_05995"
     CDS             2910..4601
                     /locus_tag="PKH10_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding EGF-like domain-containing
                     protein"
                     /protein_id="HNT27713.1"
                     /translation="MRWLMIMSIILMAFACDEGSQKKYPDMGSLTDSDAIAAVCGDGT
                     VAGGEVCEPDDTADCATVKPDTQGTATCLVDCSGWDTTACVGADECAAEPGICGDPEY
                     AACENTDEGYQCTCSNGYIPTSDGKLCVPRYDLEGPIHHGDILLIVNEDADGSNQAYI
                     GTMPDTFLTLAMPRTGTLPSDTVDLPPVRVRDRVLPLPPHATPDLRLGIARAIEPSPD
                     PEVGEVRTFWTSDLSTNLRYQIEAEALHVGTHCIVWGQIPVVVSSTTAQSLGKEFDNT
                     IYELITTNFYEASDVDQNGKITILFVDTDGQAGGYFSPYELYDLEDSNKADMIYIEQM
                     IAQWGGSTYAADVVAHEFTHLVHNNRDDLVEKKPNDNMWMWEGLSTSGEHVYRGFQRD
                     YKSTYNDSTTVAEGLSVTYWDYYGAEIEANYALTYLFHQYLRIQLGQGNAFYRELIES
                     LNNGPTDYDDLIHEYIGEELDLAQMLTGFRVALITNEKTGLYGFKGETGFTGFERKYW
                     TGNGGNEFYLRGGGALQIRLTQPFRAPKDAEKTVRFIGIHSGTVTKDTREDYENE"
     gene            4672..>5482
                     /locus_tag="PKH10_06000"
     CDS             4672..>5482
                     /locus_tag="PKH10_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27714.1"
                     /translation="MRALMLALLVLVAAGLNAERVYHADGRSFDMPPVDGVTALTGPA
                     AAVPDGAAIKRWRAGEARYLITRGTGGLPVYRYGRGYVVARPELFWRGAGDVGVLAER
                     YGLTLVEILPGYNLYKFTVNGDAVVTARRITEEGFGWAFPNLVREVQPRLALPQDALY
                     ADYQWALHNRGFYNDAYGDPVETLKRADIRFEDALRFLMKVNFEPAKYATKVAIMDSG
                     VVTTHPDLVGDGGKMDPGYDAIRDVEGGDPHIPDGASGYELATYAHGTDCAG"
BASE COUNT         1097 a         1655 c         1793 g          937 t
ORIGIN      
        1 cacgcgggga tcgcggtcaa gggggtcgac acccacggac tcgcccagcg cggcggcatc
       61 gtggtgtcgc atctgcgcat cggcgatgag gtatattcgc cgctcatccc cgaaggcgac
      121 accgacatcg ccgtggcgct cgaacggcat gaggcgctcc gggcgctgaa cggctttgtg
      181 aaggatggcg ggacgctcgt ctggtacgac accgtctggc aaccgctcga ggtgcgcgtc
      241 ggcgcgacgc cggaagtggg gcgcgaaacg gtcgcggagg aatgcgaacg ccgcacggtg
      301 aagaatgtcc gggtacgggt cgacgatctg cctgacacgc ggatgcagaa tatcgccctc
      361 atggcggccg tatgcaggga ggggctcatc cccggcatca aaaaagagca ctacgaagcg
      421 gcgatgggcg atctgatgga ggggcggatg ctcgagaaga accgcgccct tttcactcgc
      481 ctgatcgggt gaccctcatg cagcacgagt gggtccagat actcgacatg gtcgccgaag
      541 ggtgcttctc tgcggcgggt cggcgccata tccatgacct tgccccgctg tcgcgcggcg
      601 aggccgagcg ggagttccgg ctcatcggcg agaccgccac actctttgac acgggtgtcg
      661 cgatattcaa ctatgacctt gaccgggttt ccggtgtcgt ggaacggttc cggcgcgacg
      721 agtcgctcga accgatggat taccgtgtcg tcggcgatct gctcgaacaa ttgcgacgcg
      781 cgaaacagga ggtgggacgg cagacctggg tgcgtgagtt cgccctgctg ttcgcgccct
      841 tcgagcccga tgtggggctc gagatgacca tcaagcagac ggtcgataaa aagggggaga
      901 taacttccga cgccagtccc gaactcgccc gcatccgtcg gcatatcgcc gatacccacc
      961 aactcatcgg gcgggagata aagcggatga tcgccgaccc ggcggtcggc ggcatgttgc
     1021 aggaggacta tttcacgata cgcgacgacc gctatgtcct gccgttcaag acgcagttca
     1081 agcgggtgat gaacggggtc gtccataacc tgtcacggtc gggacagacc gcgttcctcg
     1141 agccgcttgc gctcgtcgaa ctcaacaacc ggctgagtct cctcgcatcg cacgaacaga
     1201 atgagatact caaggtgctc cgcgagctgc gcggtctcct cggccgccgc atcgaccatg
     1261 tcgaagcgtg cgtcggcctc gcgctccggc tcgaatcgct ctatgtccgg caacgctgga
     1321 tgcgctcata cgactgcgcg gtgcccgaat ttaccgacga gggggcgaca gccgacgagg
     1381 tctggtatcc accggtgctc gcggcgctga agaagggaac ggtgcgcaac gccttccggt
     1441 tcaaggaggg tgaacgcatc atggtcatca gtggccccaa cgcgggcggc aagacggtcg
     1501 cgctgaagag ttttcacacc gtggtcgagc tggcgcggcg cggcatcccg gtgccggcga
     1561 aacgggcgag gatacctttt ttcgacgagg tgttcctcgt gctcggcgat caacaggacg
     1621 ccgccgaggg ggaatcgtcg ttctcggctc acctgcgcaa cctcgccgcc atcgcccacc
     1681 gggcgggcga gcggtcgctc gtgctcgtgg atgagatcgg gaccggtacc gacccgcagc
     1741 agggcggggc catcgcccgg gcctttctgg aattcctgcg cgaccgccgt tcattcaccg
     1801 tcgtcacctc gcaccttgcc gaggtcaagg cgatagcgct cgaggatccc gccttcgtgc
     1861 cggtcgcgat ggggttcgat gaaaagaacg aacggccgac ctaccggttc atgtatcatc
     1921 tggtcggcgg cagcaacgcg ctcgcgctcg tccgccggat ggattttccg ccggaactgg
     1981 tccgcctgct cgagaatctg ctttcatcgc gtgacggctc catcgaaccg ctcctcaacc
     2041 gtctgcggca gaaggagcag gaactcgacg aggagcagga gcgtatctcg gcgctcaagg
     2101 ccgaaacggt caagcagaag gaagagatcg cggcgctcga acggaaactg gcgcagaaag
     2161 agcgcacctt cgaacaggaa cggatgaaga tgctcaagaa actcatggaa ctcgaggagg
     2221 cgcagttgcg ccgccgggtc gaggagaccg acgcgaagaa cgcggcgaaa cggatcggca
     2281 tcgtacgcaa ggaaaaagag gccctcgcgc aggccatcga acgggaaaag gcgaaggcgg
     2341 acgagacgcc cggggtgccg cttgccgagg ccgaaggagt gcgggtcggt gaaacggtcg
     2401 tgtatgaccg gctgacgaag ataaaaggat ctctttcggc gctcaagggg gcgaaggccg
     2461 aggtgacgac gggtggaaag agcgtgctcg tgccggtcga gcggctcata gtggtcggtg
     2521 aaaagaggaa aaaacgggcg gtcgcggctc cactggatac cggagcggcc tgttttgaga
     2581 aatgcgatgt gcgcggtatg acggtcgagg atgcctgcgc gctcgtcgaa aagacggtgg
     2641 acggcgccta tgcggcgaaa agcatcgcgg tcgccgtcgt tcacgggcac ggtaccggcg
     2701 cgctcaaaaa aggggtccgg agttatctgg aacagttggc cgggcggtac catttcgtct
     2761 ggcggagcgc tccgaccgag cagggagggg acgggactac gctcgtagaa ttctcttaac
     2821 tatttgatat gattatttta aaaaacaagg aggggtgttt gcatcttctt gagtgggtga
     2881 ggacagaaca ccttgaacgg aggtatccca tgcgttggct gatgataatg tcgatcattt
     2941 tgatggcatt cgcttgcgac gaaggatcgc agaagaaata ccccgatatg gggtcgctca
     3001 ccgacagtga cgcgatcgcg gcggtatgtg gcgatggtac ggtcgccggc ggcgaggtgt
     3061 gcgagcccga tgacaccgcc gattgcgcga cggtgaagcc ggatacgcag gggaccgcga
     3121 cctgcctcgt cgattgttcg ggatgggata cgaccgcctg tgtcggtgcc gacgagtgcg
     3181 ccgcggagcc ggggatatgc ggcgatccgg agtacgccgc ctgtgagaac accgacgagg
     3241 gctaccagtg cacctgttcg aacggctaca tccctacgag cgacggtaaa ctctgcgtgc
     3301 cgcgctacga tctcgaaggt ccgatacacc acggcgacat ccttctcatc gtgaacgagg
     3361 acgccgatgg cagcaatcag gcctacatcg gcacgatgcc tgacactttc ctgacgctcg
     3421 cgatgccccg gaccgggacg ctcccgtcgg acacggtcga tctgccgccc gtcagggtcc
     3481 gcgaccgcgt cctgccgctc ccgccgcacg ccacccccga cctgcggctc gggatagcgc
     3541 gggcgataga gccgtcgccc gatccggagg tgggcgaggt ccgtaccttc tggacctccg
     3601 acctcagcac caatcttcgc taccagatag aggccgaggc gctccatgtc gggacccact
     3661 gcatcgtctg ggggcagata ccggtggtgg tctcctcgac gaccgcgcaa tctctcggca
     3721 aggagttcga caacaccata tacgaactca tcacgaccaa tttctacgag gcctccgatg
     3781 tcgaccagaa cggcaagata acgatactgt tcgttgacac cgacgggcag gcgggcggct
     3841 atttcagccc gtatgaactg tacgacctcg aagattcgaa caaggccgac atgatctaca
     3901 tcgagcagat gatcgcccag tggggcggtt ccacctacgc ggcggatgtc gtggcgcacg
     3961 agttcaccca cctcgtccac aacaaccgcg acgatctcgt ggagaaaaaa cccaacgaca
     4021 acatgtggat gtgggagggg ctgtcgacga gcggtgaaca tgtctatcgc gggttccaac
     4081 gcgactacaa gagcacctac aacgattcga cgacggtggc cgaagggctg tcggtcacct
     4141 actgggacta ctacggtgcc gagatagagg ccaattatgc gcttacctac ctgttccatc
     4201 agtacctgcg catccagttg ggacagggga acgcgttcta ccgcgaactc atcgagagcc
     4261 tcaacaacgg cccgaccgat tacgacgacc tgatacacga gtatatcggg gaagaactcg
     4321 atctcgccca gatgctcacc ggtttccgcg tcgcgctcat caccaacgag aagacagggc
     4381 tctacggctt caagggggag accgggttca ccggtttcga gcgcaaatac tggaccggca
     4441 acggtggcaa cgagttctat ctgcgcggcg gcggcgccct gcagatacgg ctcacccagc
     4501 cgttccgcgc cccgaaggac gccgagaaga ccgtgcggtt catcggcatc cacagcggca
     4561 ccgtgaccaa ggacacgcgc gaggattacg agaacgagtg atattcttgc catttctgag
     4621 cggttcggct aatctgacac ccgatatcga acattcgcgg agggatatct catgagagcc
     4681 ctgatgttgg ccctgctggt ccttgttgcc gccggtctga acgccgagcg ggtctaccat
     4741 gccgacggtc gttccttcga catgccgccg gtcgacggcg tcaccgcgct caccgggccc
     4801 gccgccgccg ttcccgacgg cgcggccatc aaacggtggc gggctgggga ggcacggtat
     4861 ctcatcaccc gcgggacggg tggcttgccg gtctaccgct acggccgggg ctatgttgtc
     4921 gcgcgtcccg aactgttctg gcgcggcgcc ggcgatgtcg gggtgctcgc ggaacggtac
     4981 ggtctcacgc tcgtcgagat actgcccggc tacaacctct ataaattcac ggtgaacggc
     5041 gatgcggtcg tcacggcgcg ccgcatcacc gaggaagggt tcggctgggc cttcccgaac
     5101 ctcgtccgcg aggttcagcc gcgtcttgcc ctgccgcagg acgcgctcta cgccgactac
     5161 cagtgggcgc tccataaccg tggcttctat aacgatgcct acggcgatcc ggtcgagacg
     5221 ctgaaacggg ccgacatccg gttcgaggat gccctgcggt tcctgatgaa ggtcaatttc
     5281 gaaccggcga agtacgcgac caaggtcgcc atcatggact cgggggtcgt gacgacccac
     5341 cccgacctcg ttggcgatgg cggtaagatg gaccccggct acgacgcgat acgcgatgtg
     5401 gagggcggcg acccgcacat ccccgacggg gcgagcggct acgaactggc gacctacgcg
     5461 cacggcaccg actgcgcagg ta
//
LOCUS       DAOQUE010000154         5474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16406_length_5474_cov_6.284739, whole genome shotgun sequence.
ACCESSION   DAOQUE010000154 DAOQUE010000000
VERSION     DAOQUE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16406_length_5474_cov_6.284739"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PKH10_06005"
     CDS             complement(<1..859)
                     /locus_tag="PKH10_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27715.1"
                     /translation="MKVGVMHIVVKGIVWPIQIIRRKVFDGNTPESLTGGRIFRINYC
                     KFFFFYTQGLVEFLFVFRHPVSRHIKLCISGIFPIVSDHPEHDPILGRYPLIFFLIII
                     PRRNQEAYGHIDEVVQIEIDRFGKGRFLGARRFRFVHLEFDAIGIRGPFGQTGRHRIL
                     SVFGRGIFDGRYRGLVSAQASLGTVRLFDLNTGTVRYMNDKIAFAPTIIFPVRLYHGL
                     GPRTRCFSVAHIHGIGSRRGILDQNRRVRDSVTLNLCFNASGHRQMSGDRKKKGILGA
                     AGDRHGILAV"
     gene            complement(947..1528)
                     /locus_tag="PKH10_06010"
     CDS             complement(947..1528)
                     /locus_tag="PKH10_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27716.1"
                     /translation="MDNLLRLSVAFIEKILFPIQPMHTLEIPSAQLLIVRQSADMKRI
                     FPLDGHDLLFRISRHYFLRRGRAAATAAARGGSRGTSERTIVRTDKTVPHVNQFVPVY
                     GYKGGFCRSVYPVPETILSVPGKAYGLLWFGESRGLIGDAPVHHEIVLRGFESVRYLG
                     QRFSVFELYRSGTKISWCPSRIIDQTVGHIGDP"
     gene            2651..>5474
                     /locus_tag="PKH10_06015"
     CDS             2651..>5474
                     /locus_tag="PKH10_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNT27717.1"
                     /translation="MYIKNSRFETALDTSGTIYVPSKDGTEVYVFDREGYHLQTLDAL
                     SGTVRYTFDYDLTCTETINGNSYDTRAVSSITDRFGRTTTITRDVDCAATAITSPNGV
                     VTYLQTDAQGNLSSVSEPNSASVRHAMTYYSDQKGLLQSIIDPKEGVKRVYYTDEGMF
                     EREIDALGYQKTLLQSSSNPAVKMFFSAEGIKTEYKTTDNGKEVTFADGTKNVQEHKD
                     GGDVVTYADGTKVTTKKTMDDRLGGSAEHVTSHTVATGDIDAYTMTVDREVDMTDTDE
                     VPDDDVPLVFKFDKITDTVVKNGRTNTVTLTNNYDGTYTEEYLAPAPAQHTRSFTALF
                     DDKGRLLERSSSGVTEPVEFAYYDAVNDPEGAKYKIGAITQGTENDTRRKILVTYYNE
                     TNSGTGAFKGAVSSVSRLVDVDGSTEKFLTTLYYYDSHGWGSSITELDENDDPIGTIE
                     YGYDANGNVISVTPMNDVDHFFFYNALDRADYYQPPAISGVQMKTHYAYDKDGRVSDI
                     IKPDDKWIHFRYATMSDGYTGNDLTGKLQAVLVDNVEKVTYTYESTGGKKLSTITTAD
                     DTSTLTYGYNGALLASEAWSGAVIGTVTRTYNTDFKVTKLTVAGTEIDYTYTSDPAGL
                     LTVAEDLSIGRDEDTGRVTTTTLGSVGTEISYDADYGDLYSYDISGSATDYGYAIDER
                     DNLGRITERTESIGGTPTTYTYTYDEAGRLESVYDGTTTKSYEYDENGNRETYLENST
                     PVATATYDDQDRMLTYGSNTYTYTLNGDLYTKVAGGNTTTYTHDPFGNLTSVTLPNST
                     VISYVYDGRNRLLARKTNGTVDYKLIYAGQLAPVAKLDGNNSVLETYVYATGSNSPDY
                     IINGGVNYRVVKDHLGSMRMIVRATDGAVVKSVKYDEFGKVLTESGAFDTIFGFAGGI
                     RDTDTGLTKFGARWYDPETGRWLEKEP"
BASE COUNT         1440 a         1391 c         1520 g         1123 t
ORIGIN      
        1 cgacggcgag tatgccatgg cggtccccgg cggcgcccag tatgcccttc ttctttcgat
       61 cgccggacat ctgcctgtgg cccgaagcat tgaagcaaag gttaagagtt acactatcgc
      121 ggacacggcg gttctgatcg aggatcccgc gacgggaccc gatcccgtgg atgtgagcaa
      181 cggaaaaaca acgggttctt gggccgagac cgtgatagag acgaacgggg aagatgatgg
      241 tcggggcgaa cgcaatctta tcgttcatat accggacggt accggtattg agatcgaaaa
      301 ggcggacggt accaagggaa gcctgagcgg aaacgaggcc acgataacgg ccgtcgaata
      361 taccgcgacc gaaaacggac aggatgcgat gccggcccgt ttgcccgaag ggaccgcgta
      421 tacctattgc gtcgaactca aggtggacga agcggaagcg gcgggcgcca agaaaacgac
      481 cttttccaaa ccggtctatc tctatttgga caacttcatc gatgtgcccg taggcttctt
      541 ggttccgtcg gggtattatg ataaggaaaa agatcaatgg gtagcgtccg aggatgggat
      601 cgtgctccgg gtggtcggaa actatgggga agatcccact tatgcacaac ttgatatgac
      661 gggagacggg gtggcggaaa acgaacagga actcaacgag gccttgggta tagaaaaaga
      721 agaacttgca ataatttatt cgaaatatcc ggcctccggt aagactttct ggcgtgttcc
      781 cgtcaaacac ttttcgcctt atgatctgaa ttggccatac tatgcctttg acaacaatgt
      841 gcatgacccc gaccttcatc tcgcggctgg cagtaagccg aaagagaagc catgcgtaaa
      901 atactcgtct gtcattgatt gtgagaatgg cactttcgga gaaactttac ggatcaccga
      961 tgtgaccgac agtttgatct ataattcggg acggacacca ggagatcttc gtaccagaac
     1021 gatagagttc gaagacagaa aagcgttgtc cgagatatcg gacactttca aagccgcgca
     1081 gtactatctc atggtggacg ggtgcgtcgc cgatcaagcc gcgagattcg ccgaaccaca
     1141 gcagcccata cgcctttcct gggacggaga ggattgtttc gggaacagga taaacggaac
     1201 gacaaaagcc acccttatag ccatatactg gtacgaattg gtttacatgg ggaaccgttt
     1261 tatcagttcg aacaatcgtt ctttcggaag tccctcggga acctcctcgg gcggcggcgg
     1321 tggcggcggc tcttcctctt ctcagaaaat aatgccggct tatgcggaaa agaagatcgt
     1381 gcccgtcaag cgggaatatt cttttcatat cggcggattg tctaacgatc agaagctggg
     1441 cggatggaat ttcgagagta tgcatcggtt ggatagggaa aaggatcttc tctataaagg
     1501 cgacggagag acgcaacagg ttgtccacga gaactcgatg atagatgtgt tctgtagggt
     1561 aggagagaat gagtcattta ttcttcggga gggcattcct cctgaatcgg caaaagcttt
     1621 ttgttcgacg gggattggca gttttgccgg atatgcaacg agcggtggtg gtagcatagt
     1681 ggggcttata ggcttaaccc aaaacggttc ttcctgcata ataactccat accccgccaa
     1741 ccccaatgaa aatggcgtca ttctcagaca ccacaaggat gggactctct atgaaatgac
     1801 ctcatcgggc aagatattct cttataagga tggaaaatac acacatcttg cgggaagtcc
     1861 ctcgggcgaa gataggccgc tctccggtaa cgatgcccga aatattctta cgggttctgg
     1921 ctttttggca tcattcgagg ttctggaaga taaaacgatc tacttctttt ttgtgaataa
     1981 cgactactcc aatgtataca aagtatatcc tgatggaagt ttcgtcaggc tcaacccgaa
     2041 aaacgctggt agttggactt cttctccaat gagtaaccca tcgtataaag aagattttcc
     2101 cttgtccgat gccgacatgt tcaccttccc gttatacttc aacacacatt atgcgaagaa
     2161 cgggaaaata atctttgctc ttacatatga ggggtgcgct ttcgaaatag atccctctac
     2221 caatatagcc aggagatctc tgggggatcg ttgttattcg agcagttaca attcttggaa
     2281 cggggtactc tcttatgaag gtcagaatgc tgtcggctca aacggttcag attcttggac
     2341 gaagggattt ttcatcagca acatcggctc tctctctgat ggtacgatac tgtatatggt
     2401 aaggttgaaa tatcctcacg agggaacagc aaccataaaa aaaagacatg tcgtcgcggt
     2461 tagatacgat gaagcaaaac aacaagatat catgagtcgc tttgccgggg acaacgcgct
     2521 tctcctgcct tccgagcccc tctccggccc cgtactcgaa acgggcatct tagacacttc
     2581 cttggtggtc gatcccacgg atcatgtgtt ttttgcgata ggcggggcga gcacttaccg
     2641 ccggaatttc atgtacataa agaatagtcg tttcgagacg gcactcgata cttccggcac
     2701 gatttatgtt ccctctaaag acgggacgga ggtctatgtc tttgaccgag agggatatca
     2761 tctgcaaacg ctcgatgcgt tgtccggaac ggtgcggtat acattcgact atgatcttac
     2821 ctgtaccgaa acaataaacg gcaatagtta cgacacgcgt gcggtaagtt ccatcaccga
     2881 ccgattcgga agaacgacga ccataacccg cgatgtcgac tgcgcggcga ccgccattac
     2941 ttcgcctaat ggggtcgtca cctatcttca gaccgatgca caagggaacc tttcctccgt
     3001 gagtgaaccg aattccgcct ccgtgcgtca cgcgatgacc tattattcag atcagaaggg
     3061 cctgttgcag agcatcatcg atccgaagga gggcgttaag agggtatatt acaccgacga
     3121 agggatgttt gaacgggaga tagacgctct cggctatcag aaaacgctgc tccaatcgtc
     3181 gagtaacccc gccgtaaaga tgttcttttc ggccgaaggg ataaagaccg aatacaagac
     3241 gacggacaac ggcaaagagg tgaccttcgc cgacggtacc aaaaatgtac aggaacacaa
     3301 agatggcggg gatgtcgtca cctacgccga cggaacgaag gtcaccacga aaaagaccat
     3361 ggacgaccgg ctcggcggca gtgcggaaca tgtcacctcg cacaccgttg cgacgggcga
     3421 catcgacgcc tacacgatga ccgtcgaccg cgaggtcgat atgaccgata ccgacgaagt
     3481 tcccgacgac gatgtgccgt tggtattcaa gttcgacaag ataaccgata ccgtggtcaa
     3541 gaacgggcga acgaataccg ttacccttac gaataattac gacgggacct ataccgaaga
     3601 gtatcttgct cccgcgccgg cgcaacacac cagaagtttc accgctcttt tcgacgataa
     3661 agggagattg ctggaacggt cctcgtccgg cgtgacggag ccggtcgagt tcgcgtacta
     3721 tgacgcggtg aacgatccgg agggggcaaa atacaagatc ggcgccatca cgcagggaac
     3781 ggaaaacgac acccggcgga agatactggt cacctattac aacgagacca acagcggtac
     3841 gggcgccttc aagggggctg tcagttcggt ctctcgtctg gtcgatgtgg acggaagtac
     3901 ggaaaaattc cttaccactc tttactatta cgatagccac ggatggggta gcagtatcac
     3961 ggaacttgat gagaacgatg acccgatcgg caccatcgaa tatgggtacg acgcaaatgg
     4021 caatgtgata tcggtcacgc cgatgaacga tgtcgatcat ttcttcttct acaatgcgct
     4081 cgaccgggcc gactactatc aaccgccagc gatctccggc gttcagatga agacccatta
     4141 cgcgtacgac aaggacggca gggtaagcga catcatcaag ccggacgaca aatggatcca
     4201 tttcagatac gcgacgatga gtgacgggta caccggtaat gacttaaccg gcaaattgca
     4261 ggcggtcctt gtggacaatg tggaaaaagt gacgtatact tatgaatcga ccggcgggaa
     4321 aaagttatcg acgataacga cggcggacga cacgagtacg ctgacctacg gatacaacgg
     4381 ggcgttactt gcaagcgagg catggagcgg agcggtgatc ggcacggtga cgcggaccta
     4441 caacaccgac ttcaaggtga cgaaactcac cgtggccgga acggagatag attacaccta
     4501 cacctccgac ccggcggggt tgctgaccgt ggcagaagac ctgagcatcg ggcgggacga
     4561 agatactggc cgcgtcacga cgacgacgct gggttcggtc ggcacggaga tctcctacga
     4621 cgccgattac ggggacctct actcgtacga catttccgga agcgcgaccg attacggata
     4681 cgccattgat gaacgggaca acctcggccg gataaccgag cggaccgaaa gcatcggcgg
     4741 tacaccgacg acctacactt acacctacga cgaagcgggc cgacttgaat cggtgtacga
     4801 cggcacgacg acgaaaagtt acgaatacga cgagaacggg aaccgggaga cctatctgga
     4861 gaacagcact ccggtagcca cggcgaccta cgacgatcag gaccgtatgc tgacctacgg
     4921 gtccaacact tacacctaca cgctgaacgg cgacctctac accaaggtcg ctggcgggaa
     4981 cacgaccacc tacacccacg accccttcgg caacctgacg agcgtgacct tgccgaacag
     5041 cacggtgata tcctatgtct acgacggccg caatcggctc ctcgcgcgga agaccaacgg
     5101 gaccgtcgac tataaactga tctatgcggg acagttggcg ccggtggcga aactcgacgg
     5161 gaacaacagc gtgctggaga cctatgtcta tgcgacgggg agcaacagcc cggattacat
     5221 catcaacggc ggggtcaact accgcgtggt gaaggatcat ctcggctcga tgaggatgat
     5281 agtgcgcgct acggatggtg cggtggtcaa gagcgtcaag tacgacgagt tcggaaaggt
     5341 gctcacagaa agcggagcat tcgacacgat cttcggcttc gcgggcggca tcagggacac
     5401 cgacaccggt ctcaccaaat tcggcgcgcg gtggtatgac cccgagacgg ggcggtggct
     5461 ggagaaggaa ccgc
//
LOCUS       DAOQUE010000155         5448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16519_length_5448_cov_4.294827, whole genome shotgun sequence.
ACCESSION   DAOQUE010000155 DAOQUE010000000
VERSION     DAOQUE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16519_length_5448_cov_4.294827"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2467
                     /gene="rpoB"
                     /locus_tag="PKH10_06020"
     CDS             <1..2467
                     /gene="rpoB"
                     /locus_tag="PKH10_06020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNT27718.1"
                     /translation="EVRDVHKTHYGRICPVETPEGPNVGLIVSLTTFAAINEFGYIET
                     PYRVIEKDKATGKSKVTDKVIYLDAIKEQEHRIVPSSVSVDKNGLLMEDLVNARDKGD
                     IVRVRSEEITLMDLAPVQIVSVSAALVPFLEHDDANRALMGSNMQRQAVPLVRAEAPL
                     VGSGMEQFVARDSGYTVYAKRGGEVVKVDATHVVIKVDPAETEEAIDVDIYNLSKYRR
                     SNQNTAVDQRPVVNVGDTIKKGDVIADGPAIDRGELALGRNVLIAFMPWSGYNFEDSI
                     LVNERLLHEDAFTSIHIEEFVCEARDTKRGPEEITADIPNAPDEVLRKLDESGIIRIG
                     QKVRAGDILVGKVTPKGETVLSPEEKLLRAIFGEKAGDVKDSSLYVRPGVEGVVIDAR
                     IFARKGTKKDNRMKEQEKEQFDTLKRARDEEIRIIEVHTYEMIRKLLIGKTTDAPVTH
                     EKGRSLIKKGEKLTADGVSKIPESYLENVSVSDRNAMKKIKESILAMRRKIETVKKIY
                     DTKTKQISKNDDLAPGVIKSVKVFVAIKRKLSVGDKMSGRHGNKGVVSLLLPEEDMPY
                     MADGRTVDIVLNPLGVPSRMNIGQILEVHLGLTARRIGEKINAHLAQNWPSKKLKDEL
                     KKLLDPSPTVAKIIDEADDDDVRSFIRSMKRGIHTASPVFDGAKDADIHNLLEYADQP
                     LDGKVTLFDGRTGDPFESDVTVGIMYFLKLHHLVDEKVHARSTGPYSLVTQQPLGGKA
                     QFGGQRLGEMEVWALEAYGAAYSLQEFLTVKSDDVEGRKKLYESIVRGKHKYDIGVPA
                     SFSVLVKEMQSLCLDVTLVEENR"
     gene            2495..>5448
                     /gene="rpoC"
                     /locus_tag="PKH10_06025"
     CDS             2495..>5448
                     /gene="rpoC"
                     /locus_tag="PKH10_06025"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNT27719.1"
                     /translation="MKDIFKIFERPKSSFDFKAMEVALASPEKILEWSYGEVKKPETL
                     NHRTFKPEKDGLFCAKIFGPVNDFECLCGKYKRMKYKGMVCEKCGVEVIESKVRRERM
                     GHINLASPVAHIWYVKTSPFYVGTILGISNAHLNSVIYCEGYLVLESKIPGVKKGEYL
                     EEETYHEYLEKFGEDALVAETGAAPVRKLLESENLDLVTLKAELETAITQTGDKSFIA
                     KQQKRLNVVNKFLRSNIRPRDMVLTILPVLPPDLRPLVPLDGGRFATSDLNDLYRRVI
                     NRNNRLKKLMDLGAPDVIIKNEKRMLQEAVDALFDNTKVHRPIQGPSGRPYKSLSDML
                     RGKQGRFRQNLLGKRVDYSGRSVITIGPELKLHQCGLPKIMALELFKPFIYNKLQDRG
                     FASTVKGAKQMVENGEDIVWEILEEVVREHPVLLNRAPTLHRLGIQAFEPILIESKAL
                     RLHPLVCTAFNADFDGDQMAVHVPLSVEAQLEARVLMMSTNNILSPASGAPIIVPSQD
                     IVLGLYYLTTERPNAKGEGKYFYSEKEVLMARDAGAVEWQTMLFVRIDGTIHRTTPGR
                     VLLWRVTPREIGFDMINRQFKKSNWSDILDLSYRRSGGKATVIFADKLKNLGYEFSTR
                     AAYSIALSDIKVPKEKWEIIEETEREILEIEQHYQGGGLSKGERYNQIVDLWDRANER
                     VTSKMMEQLKEKEVVHEKWGKVTIPSDNPIYVMADSKARGSKDQIRQLGGMRGLMAKP
                     SGEIIETPIISNFKEGLDSLDYFISTHGARKGSADTALKTANSGYLTRRLVDVAQDVV
                     VVEDDCGTTMGVYAEPLLNDSGEIISPLRERVLGRIVMDDVEDPETGEIIIESGEMID
                     EEKAEKITALGIDSIYIRSTLTCRSKYGVCAKCYGRDLSRGHLINVGEAVGIIAAQSI
                     GEPGTQLTMRTFHVGGVASRTSEKSSHEAKRGGTIRFKDIKLLAKHKDKAAAKDKSRK
                     DGLFVAMNRNGR"
BASE COUNT         1294 a         1494 c         1616 g         1044 t
ORIGIN      
        1 cgaggttcgt gatgtccata agacccatta cggacgcatc tgtccggtcg agacgccgga
       61 gggtccgaat gtcggtctta tcgtttcact caccaccttc gcggccataa acgagttcgg
      121 ctatatcgaa acgccgtatc gcgtgataga aaaggacaag gcgaccggca agagcaaagt
      181 gaccgacaag gtcatctacc ttgacgccat caaggagcag gagcatcgga tcgttccctc
      241 ctcggtgtcg gtcgacaaga acggtctgtt gatggaagat ctggtcaatg cgcgcgataa
      301 gggcgacatc gttcgcgtgc gcagcgaaga gatcaccctc atggaccttg ctccggtgca
      361 gatcgtttcg gtatcggcgg cgcttgttcc cttcctagaa catgatgatg cgaaccgtgc
      421 cctcatgggc tcgaacatgc aacggcaggc ggtcccgttg gtgcgcgccg aggcgccgct
      481 tgtcggttcc gggatggaac agttcgtcgc ccgtgattcg ggctacaccg tgtacgccaa
      541 acggggcggc gaggtggtca aggtcgatgc cacgcatgtc gtcatcaagg tcgatccggc
      601 ggagaccgaa gaggccatag atgtcgatat ctataatctc tccaaatacc ggcggtcgaa
      661 tcaaaacacc gccgtggatc agcgccccgt ggtgaatgtg ggcgatacca tcaagaaagg
      721 tgatgtcatc gccgacgggc cagccatcga tcgcggcgag ttggcgctcg ggcgcaatgt
      781 gcttatagcc ttcatgccgt ggagcggcta caatttcgag gattcgatat tggtgaacga
      841 acgactgctc cacgaggatg ccttcacctc catccacatc gaggaattcg tctgcgaggc
      901 ccgcgacacc aagcgcggtc ccgaagagat aacggccgat atcccgaatg cgcccgacga
      961 ggtgctccga aaactcgacg agagcggcat catccgcatc ggtcaaaagg tccgggcggg
     1021 cgatatcctc gtgggaaagg tcacgccaaa aggcgagacg gtcctgtcgc cggaagagaa
     1081 actgctccgc gccatcttcg gcgagaaggc gggcgatgtg aaagacagct ctctctatgt
     1141 ccgacccggt gtcgaagggg tcgttatcga tgcccgcatc ttcgcccgga aagggaccaa
     1201 gaaggacaac cggatgaagg agcaggaaaa agagcagttc gatacgctca agcgggcgcg
     1261 tgacgaagag atacgcatca tcgaggtaca cacctacgag atgatccgca aactgctcat
     1321 cggcaaaacg accgatgcgc ccgtcacgca tgaaaagggc cgctctctca taaagaaagg
     1381 ggagaaactg accgctgacg gcgtgtcaaa gataccggaa tcctacctgg agaacgtcag
     1441 cgtttccgat cgcaatgcca tgaagaagat caaggaatcg atcttggcga tgcgccgcaa
     1501 gatcgagacc gtgaagaaga tatacgacac caagaccaaa cagatatcca agaacgatga
     1561 cctggcgccc ggcgtcatca agtcggtcaa ggtcttcgtc gccatcaagc gcaaactgtc
     1621 cgtcggcgac aaaatgtcgg gccgtcacgg taacaagggc gtcgtgtcgc tcctgttgcc
     1681 tgaggaggat atgccataca tggcggatgg ccggaccgtc gatatcgttc tcaacccgct
     1741 cggcgttccc agccgcatga acattggtca gatactcgag gtccatctgg ggctgacagc
     1801 gcgccgtatc ggcgaaaaga tcaacgcgca cctcgcccag aactggccca gcaagaaact
     1861 caaggacgag ctcaagaaac tgctcgaccc ctcgccgacg gtggcgaaga tcatcgacga
     1921 ggccgatgat gacgatgtcc gttcgttcat ccggtcgatg aagcggggta ttcataccgc
     1981 ttctccggtc ttcgacggcg cgaaggatgc cgatatccac aatctgcttg aatatgctga
     2041 tcaaccgctg gacggcaagg tgacactgtt cgacgggcgc accggtgacc ctttcgagtc
     2101 cgatgtcacg gtcgggataa tgtatttcct gaaattgcac cacctcgttg atgaaaaggt
     2161 ccatgctcgg agcacggggc cctattcgct ggtcacccag caaccgctcg gtggcaaggc
     2221 ccagttcggc gggcagcgac tcggcgagat ggaggtctgg gcgctcgagg cctatggcgc
     2281 tgcctactcg ctgcaggaat tcctgacggt caagtcggac gatgtcgagg ggcggaaaaa
     2341 actctacgag tcgatcgtgc ggggcaagca caaatacgat atcggtgtcc cggcctcatt
     2401 ctccgtcctt gtgaaggaga tgcagagcct ctgcctcgat gtgacgcttg tggaagagaa
     2461 ccgttaagca acagttcgga aggaggagcc cgtggtgaaa gatattttca agatattcga
     2521 gcggcccaag agcagtttcg atttcaaggc gatggaggtc gcactcgcgt cacccgagaa
     2581 gatcctcgag tggtcctacg gcgaggtgaa gaagcccgaa acgctcaatc accgcacctt
     2641 caagccggag aaggacggcc tgttctgcgc caagatcttc ggtccggtga acgacttcga
     2701 atgcctgtgc ggtaagtaca agcggatgaa gtacaaagga atggtctgtg aaaaatgtgg
     2761 cgtcgaggtc atcgaatcga aagtgcgtcg cgagcggatg gggcacatca acctcgcctc
     2821 tccggtcgcc cacatctggt acgtcaagac atcacccttc tacgtcggta cgatattggg
     2881 tatcagcaac gcccacctga actcggtcat ctattgcgag ggctatctgg tccttgaatc
     2941 gaagatcccc ggcgtgaaga aaggggagta tctggaagag gaaacctacc acgagtacct
     3001 tgagaaattc ggtgaggatg ccctcgttgc cgagacaggc gccgctcccg tgagaaaact
     3061 gctcgagagc gagaacctgg atctcgttac gctgaaggcg gaactggaaa cggccatcac
     3121 gcagacgggc gacaaaagtt tcatcgccaa gcagcagaag cgcctgaatg tggttaacaa
     3181 gttcctgcgc tccaacattc gtccacgcga catggtcctg accatcctgc cggtcctgcc
     3241 gcccgacctg cggccgctcg tgcctcttga tggtgggcgg ttcgcgacaa gcgacctgaa
     3301 tgacctttat cgtcgtgtca tcaaccggaa taaccgtctc aaaaaactga tggacctcgg
     3361 tgcccccgat gtcatcatca agaacgagaa gcggatgttg caggaggcgg tcgacgcgtt
     3421 gttcgacaac accaaggtcc atcgcccgat acaggggccg agcggtcgcc cctacaagtc
     3481 gctttccgac atgttgcgcg gcaaacaggg ccggttccgc cagaatctgc tcggtaagcg
     3541 cgtcgactac tcgggtcgct cggtcattac catcggtccc gaacttaagc tacatcagtg
     3601 cgggcttccg aagatcatgg cgctcgaact gttcaaaccg ttcatctaca acaagcttca
     3661 ggatcgtggc ttcgccagta ccgtcaaggg cgccaaacaa atggtcgaga acggcgagga
     3721 tatcgtgtgg gagatccttg aagaggtggt gcgtgaacat ccggtacttc tcaatcgcgc
     3781 gccgaccctg catcgactcg gcatacaggc cttcgagccc atacttatcg aatccaaggc
     3841 gctccgactt catccgctcg tctgtaccgc gttcaacgcc gactttgatg gtgaccagat
     3901 ggcggtccat gtaccgcttt cggtcgaggc gcaactcgag gcacgcgttc tgatgatgtc
     3961 caccaacaat atcctttccc cggcatcggg tgcgccgata atcgttccgt cgcaggacat
     4021 cgtgttgggg ttgtattatc tcaccaccga gcgtccgaat gcgaagggcg agggcaagta
     4081 tttctactcc gaaaaggaag tgctcatggc gcgtgatgcc ggcgcggtcg aatggcagac
     4141 gatgctgttc gtgcgtatcg acggcaccat acaccggacg acgccgggcc gtgttcttct
     4201 ctggcgggtc accccgcggg agataggttt cgacatgata aatcggcagt tcaagaagag
     4261 caactggtcc gatatcctcg atctgagtta tcgtagatcg ggtggtaagg cgaccgttat
     4321 cttcgccgat aaactcaaga acctcggata cgagttctcg acgagggcgg cttattcgat
     4381 agcgctttca gacataaaag tgcccaagga aaaatgggag atcatagagg agaccgagag
     4441 ggagatcctc gaaatagaac agcattatca gggtggcgga ctcagtaaag gcgaacgcta
     4501 caaccagatc gtcgatctct gggaccgtgc caacgaacgc gtcacctcca agatgatgga
     4561 gcagctaaag gaaaaggagg tggtgcatga gaagtggggg aaggtaacga taccgtctga
     4621 caatcccata tatgtcatgg ccgattcgaa ggcgcgcggg agcaaggacc agatccggca
     4681 gctcggcggt atgcgcggac tcatggcgaa accgtcggga gagatcatcg aaacgccgat
     4741 catctcgaac ttcaaggaag gtctcgattc gctcgattac ttcatatcga cgcatggcgc
     4801 ccgcaagggt tcagccgaca ctgcactcaa gaccgcgaac tccggttacc tcacccgccg
     4861 tctggtcgat gtcgcgcagg atgtggtcgt ggtcgaggat gattgcggta ccacgatggg
     4921 cgtgtacgcg gaaccgcttc tgaacgattc gggggagatc atctcgccgc ttcgggagcg
     4981 ggtgctggga cgcatcgtga tggatgatgt cgaggatccc gaaacgggag agatcatcat
     5041 cgagtcaggc gaaatgatag acgaggagaa ggccgagaag ataacggcgc tcggtatcga
     5101 ctccatatac atccgctcaa cgctcacctg tcgttcgaag tatggtgttt gcgccaaatg
     5161 ctatggtcgc gatctttcga ggggccacct gatcaatgtc ggcgaggctg tcggtatcat
     5221 agcagcccag tcgatcggtg agccgggtac ccagttgacg atgcgtacct tccacgtcgg
     5281 cggcgtcgca tcccgcacct ctgaaaagtc ctcgcatgag gcgaagcggg gcgggacgat
     5341 acgcttcaaa gacatcaaac tgttggcaaa acacaaggac aaggccgccg cgaaggataa
     5401 gtcccggaag gacggcctgt tcgtcgccat gaaccgtaac ggtcgtat
//
LOCUS       DAOQUE010000156         5440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16544_length_5440_cov_5.016527, whole genome shotgun sequence.
ACCESSION   DAOQUE010000156 DAOQUE010000000
VERSION     DAOQUE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16544_length_5440_cov_5.016527"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="PKH10_06030"
     CDS             <1..1514
                     /locus_tag="PKH10_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27720.1"
                     /translation="PFAHQPQANGRIMTGGGPSRRVFLALLVPAACIVLLSFLRPLTT
                     GSIKVDENYLSGVPLAAAILLFVVSLAIFTLGYFRVVTRTPGHDLSALQTKHLAQLLI
                     VVSFFMLPMLSNDIFSLIAYGDTALRGNDPFTDPLALQRSQFFPFVGSAWATAPFAYG
                     PLSLYLSMFAVHFGGNIIGALAVYKLIIALFCLLFVETVSRIPGTGDGRYNLLALVTC
                     APVLWLQCAGQSHNDAASAALLAVGILLLMRHRPVAGFMFAVLAGAFKYSAFLAIPAM
                     IAAIWVTARPLRERVWILLASTLVTLATLSLLFLSIGSDSRALLFQTEFLSSKKPSKS
                     IVDTVTEVIIVSSALWERGLPASGERNDVYQSLEDRKTAIWRVAIPFMYLFSALLAFL
                     VLRRTRRDPGAFFATAAKIATIIICFASPVFQPWYFAAFLPLFAFLKDREWIAWSAII
                     LGYSNLMNIMHVIDRESSWYLAVPPFFVITTGILFFAYFRQRFLAPVTEDDSR"
     gene            1511..3097
                     /locus_tag="PKH10_06035"
     CDS             1511..3097
                     /locus_tag="PKH10_06035"
                     /inference="COORDINATES: protein motif:HMM:NF015836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27721.1"
                     /translation="MSRLVSFFSAPLLSPELKQRYGHYLLVLLALCLLTAWTTQGYLH
                     ADEYFQTIEFASYKLGITKAESLPWEFHSRIRPWLQPGIYYLILKAFSFVGIADRFVQ
                     VLLLHFLSAFLSIATLLYFGTVAFRWFRTEQEQGWLMRVLCFTFFVPLLFVRNSSEVF
                     STACFMTAATILLRRLSPDRRTDDPGFKAWEMIVPGVLCGLAFEFRYSSAFLVLFFCL
                     WLLWVARLRFLLLTTLALSSFLPILVCIRIDRWGYGDWVFAPWNYFASNILDKRMEHF
                     GTSPFFAYLYLLIGNIGPHISLLLVVAIFLCWYRHPRHVVTWVTLPYFVFHSLLSHKE
                     DRFMFPLLFLWLAAAVLAVATSQGRPVAWLSRVFQEKNRRYWNFVWYTNLAIVAFLAL
                     YPITVVPQLKMQRHLYHHHAGDTIYKFADYDPYIRKELFFSFYRPPRLRIEQLPDTAQ
                     LESIARGADRPLYVATLLPGLTEHSDYLRHHATPEFRSFLFCRESFAPVSRKLREFFP
                     HHLCPVLYRIEPTNTGPTDR"
     gene            3152..4078
                     /locus_tag="PKH10_06040"
     CDS             3152..4078
                     /locus_tag="PKH10_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT27722.1"
                     /translation="MMRDDAPQSPRASEMGQQQQLSDIKEIRKLVVQSLNVANALDIS
                     LRALNTKVNQKDKSELNKSFFLLVMLGVVVVLSFFFYFRAEVTHFKEKLGTAAKQNEY
                     LQKDIKELKERLATSENTDIKAFNLYLAFKEGDPDQAIKKYAEFNLSALSRLERFVIE
                     TEVNLIKQKAAINKYEEGVTLFNRKSYLAAVERFEESLKISGVGKHVPSLFYYTSTAY
                     YRANEIEKAAIAFERFLFVNTEKGLTKDKAELLLGVCYERMKQYERAINFYQQVLKDN
                     KYSRFGPTIRDRMRNLQKKIDKQKNMDDKRAS"
     gene            4145..4729
                     /locus_tag="PKH10_06045"
                     /pseudo
     CDS             4145..4729
                     /locus_tag="PKH10_06045"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
     gene            4774..>5440
                     /locus_tag="PKH10_06050"
     CDS             4774..>5440
                     /locus_tag="PKH10_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNT27723.1"
                     /translation="MHDTDTARRSSPHAPLPPRFSYPDDARWQACEGKRYAETVWQRE
                     LAGMWPAEGAVSPEAVTLAAACGVKWLATDEGILFRSVGAVDKKQVLYRPWRFRERDH
                     ECALFFRDKYLSDLIGFSFAKAETGQAVESFLNHVRSIRHAVPDTGQDPAITVALDGE
                     NAWENYPDNGRDFLHGLCKTLADAPDIATETFSGFMATNPSDAYPTLPSLHSGSWIQS
                     DYQI"
BASE COUNT         1063 a         1786 c         1328 g         1263 t
ORIGIN      
        1 ttcctttcgc tcatcagccg caggcgaacg ggaggatcat gaccggagga ggtccttccc
       61 gccgggtttt tctcgccctg cttgttccag ccgcatgcat agtacttttg agtttccttc
      121 gtccccttac caccggatcg atcaaggtgg acgaaaatta cctttcgggc gttcccctcg
      181 ctgccgccat cttgctgttc gtcgtatcgc tcgcgatctt caccctcggc tattttcgcg
      241 tagtgacgcg gaccccgggc catgacctct cagcattaca gaccaaacac ctcgctcaat
      301 tactcatcgt cgtctctttt ttcatgctcc cgatgctttc caacgatatt ttcagcctca
      361 tcgcctatgg cgacaccgcg cttaggggca atgatccctt caccgatccg ttggcgctcc
      421 aacggtccca gttcttcccc ttcgtcgggt ccgcatgggc gaccgcccct ttcgcctacg
      481 gtccgctgag tctgtatctc tcgatgttcg cggtccattt tggtgggaac atcatcggcg
      541 cgctcgcggt ctacaaactg atcatcgccc tgttctgtct cctgttcgtt gaaacggtct
      601 cccgcatacc gggcaccggg gacggtcgct acaacctgct cgcgctcgtt acctgcgccc
      661 cggtactctg gctccagtgc gccggacagt cgcacaacga tgcggcatcc gccgcgcttc
      721 tcgcggtcgg catccttctc cttatgcgcc accgtcccgt cgccggcttt atgttcgccg
      781 tgcttgccgg cgcattcaaa tactccgcgt tccttgccat ccccgccatg atcgccgcca
      841 tatgggtgac cgcccgcccc ctgcgggaac gggtgtggat cctgctcgcc tcaacgctcg
      901 ttacgctggc gaccctgtcc cttctgttcc tttcgatcgg cagcgactcg cgcgccctcc
      961 tgttccagac cgaattcctc tcgtcgaaaa aaccgagcaa gtcgatcgtg gataccgtca
     1021 ccgaggtgat catcgtctcc tcggcgctct gggaacgggg cctacccgcc tctggagaac
     1081 gcaatgatgt ctaccagtcc ctcgaagatc gtaaaacggc catctggcgc gtcgcgatac
     1141 cgtttatgta cctcttctcc gctctcctcg cctttcttgt cctgcgccgg acccgcaggg
     1201 atcccggcgc ctttttcgcg accgcagcca agatagccac tatcatcata tgctttgcct
     1261 ctcccgtatt tcaaccgtgg tatttcgccg ccttcctgcc attgttcgct ttcctgaagg
     1321 accgggaatg gatcgcatgg tcggcgatca tcctcggcta cagcaacctc atgaacatca
     1381 tgcatgtcat cgatcgcgaa agctcttggt atctggccgt gcctcccttt ttcgtcatca
     1441 ccaccggcat attgtttttc gcctatttca ggcaacgatt cctcgcaccg gtaacggagg
     1501 acgatagccg atgagccggc tggtctcatt tttctcggca ccactccttt cgccggaact
     1561 caagcaacgc tacggccact atctgctcgt tctactcgcg ctctgtcttc ttaccgcgtg
     1621 gaccacgcag ggctatcttc acgccgacga gtatttccag accatagagt tcgcgagtta
     1681 caaactgggt atcaccaagg cagagtccct gccctgggaa ttccactccc ggatccgacc
     1741 gtggctccaa cccggcatct attacctcat ccttaaagca ttctccttcg tcggcatcgc
     1801 cgatcgtttc gtacaggtac tgcttcttca tttcctctcg gcattcctga gcatcgccac
     1861 cctgctttat ttcggcacgg tcgccttccg ctggttccgc accgagcagg aacaggggtg
     1921 gttgatgcgc gtgctctgtt tcaccttttt cgttcctctt ctgttcgtcc gcaactcctc
     1981 tgaggtcttc tcgaccgcct gtttcatgac ggcggccacg atactgttaa gacgcctttc
     2041 tccggaccga cgcaccgacg atcccggatt caaggcctgg gaaatgatcg ttccgggggt
     2101 tctgtgcggc ctcgcattcg agttccgcta ctcgagcgcc ttccttgtac tgttcttctg
     2161 cctctggctc ctctgggtcg cccgacttcg cttcctcctg ctgaccacgc ttgcgctttc
     2221 ctcgttcctt cccatcctgg tctgcatccg catcgaccgc tggggatacg gcgattgggt
     2281 ctttgccccg tggaactatt ttgcctccaa tatcctcgat aagcggatgg aacatttcgg
     2341 cacttcccct ttctttgcct acctttacct tctgatcggc aatatcggtc cccacatatc
     2401 gcttcttctg gtcgtcgcca tcttcctctg ctggtaccgt cacccgcgcc atgtggtcac
     2461 ttgggtgacg cttccttact ttgtgttcca ttctctcctg agccacaagg aggatcgctt
     2521 tatgttcccg ctcctctttc tctggctcgc cgccgcggtc ctcgcggtcg ccacctctca
     2581 gggcagaccg gtcgcatggc tttcgcgggt attccaagaa aagaaccggc gctactggaa
     2641 cttcgtctgg tacaccaacc tcgcgatcgt cgcctttctc gcactctatc ccataacggt
     2701 cgtcccgcaa ctcaagatgc agcgccacct ttaccatcac cacgccgggg acacgatcta
     2761 caagttcgcc gactacgatc cctacatacg caaggaactt ttcttctcct tctatcgccc
     2821 accacgcctt cgtatcgagc aactgcctga tacagcgcag cttgaatcga tcgcccgcgg
     2881 cgccgaccga cctctctatg tggcgaccct tctccccggc ctcactgaac actccgacta
     2941 ccttcggcac catgccactc cggaattccg ttccttttta ttctgtcgcg agtccttcgc
     3001 gccggtctcg agaaaacttc gcgagttctt cccccatcat ctctgtccgg tactttaccg
     3061 tatagaaccg acgaatactg ggccgactga tcgctagaaa cttgaaataa ttatatttct
     3121 tgctatcatt gctcgtcata aagaggaaag catgatgcgc gacgatgccc cccagagccc
     3181 ccgggcgtcc gagatggggc aacaacaaca attaagcgat atcaaggaga tacgcaaact
     3241 cgttgtgcag tcgctcaacg tcgccaacgc actggacatc agtcttcgcg ccctcaatac
     3301 caaagtgaat cagaaggaca aatcggaact caataaaagt tttttcctac tcgtcatgct
     3361 cggcgtggtc gtcgtcctat ccttcttttt ctacttccgc gccgaggtga cccacttcaa
     3421 ggagaaactc gggaccgccg caaagcagaa cgaatacctc cagaaggata taaaggaact
     3481 caaggagcgc ctcgcgacct ccgaaaacac cgatatcaag gcctttaacc tctacctcgc
     3541 cttcaaggag ggagatcccg atcaggcgat aaaaaaatac gccgaattca acctctcggc
     3601 cctctcgcga ctcgagcgat tcgtcatcga gaccgaagtg aacctcatca aacagaaggc
     3661 ggccatcaac aagtacgagg aaggggtaac gctctttaac cgcaagagtt atctcgcggc
     3721 ggtcgaacgg ttcgaggaaa gtctcaagat atcgggcgtc ggcaaacatg tgccgagcct
     3781 cttctactac acctccaccg cctactaccg ggcgaacgag atagagaagg cggcgatcgc
     3841 ctttgagcga ttcctgttcg tcaataccga aaagggactc accaaagaca aggcggaact
     3901 gctgctcggc gtctgttatg aacggatgaa acagtacgag cgggcgatca atttctacca
     3961 gcaggtactc aaagacaaca aatactcgcg attcggaccg accatccgtg atcgtatgcg
     4021 caacctgcag aagaagatcg ataaacaaaa gaacatggac gataaacggg catcgtaaca
     4081 tctctcgccg acggagacgc gcctcatgac cgagcggccc atcgccctgc tcttcctttg
     4141 gcatatgcac cagcccttct acaaggacgc ccagaacggc tcctacctca tgccatgggt
     4201 acgactccac gccacgaagg gctactatga catacctcag gtcatggaac gatacggcat
     4261 caagggctgc gtgaatgtgg tcccctcgct catcgagcag atcgaggatc acctgcagga
     4321 ccgggtgacc gaccgatgga agacgctcac catcaagcgc cccgaggaga tgagcgagga
     4381 ggagaaggcg ttcgtcatcc gcaacttctt catgctctgc tgggaccgac tcgtgaagac
     4441 ctccccccgt tactgggaga tactctccaa acgcgagcgg ttcgtgggga aactctcgtg
     4501 gaccgagatg acccggttct tctcggacga cgaattgcgt gacctgcagg tcctcttcaa
     4561 tctcaaatgg ttcggcttca ccgcgcgtga acgatacccg cttctccgga aactcgacga
     4621 gaaggaccgc agcttcaccc acgacgagaa gatggacctc ctcagaacgc aggagacggt
     4681 gcttcgtgag atactgccgc tgtatcgccg cctcgctgat gaggggatca tcgagctggc
     4741 cttaccccct tctaccatcc catcctgccg cttatccacg acaccgatac ggcccgccgt
     4801 tcatcgccgc acgcaccgct tccgccacga ttctcctacc ccgatgacgc ccggtggcag
     4861 gcctgcgagg ggaagcggta cgccgaaacg gtctggcaac gagaacttgc aggcatgtgg
     4921 ccggcggagg gagcggtctc gcccgaagcg gtcaccctcg ccgcggcctg cggcgtgaag
     4981 tggctggcga ccgacgaagg gatactcttc cgttcggtcg gcgcggtcga caagaaacag
     5041 gtgctctatc gaccgtggcg tttccgggaa cgcgatcacg aatgcgccct gttcttccgt
     5101 gacaagtacc tttctgatct catcgggttc tcgttcgcga aagcggagac ggggcaggcg
     5161 gtggaaagtt tcctgaatca cgtgcgctcc atccgtcatg ccgttccaga taccggccaa
     5221 gaccccgcta tcaccgtcgc tctcgatggc gagaacgcgt gggagaatta tcccgacaac
     5281 ggtcgcgatt tccttcacgg actctgcaag acgctcgccg atgcccctga tatcgccacc
     5341 gagaccttct cgggcttcat ggcgacgaat ccttcggatg cctacccgac gcttccttcg
     5401 ctccatagcg gctcatggat acagagtgat taccagatat
//
LOCUS       DAOQUE010000157         5435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16563_length_5435_cov_3.889591, whole genome shotgun sequence.
ACCESSION   DAOQUE010000157 DAOQUE010000000
VERSION     DAOQUE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16563_length_5435_cov_3.889591"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PKH10_06055"
     CDS             complement(<1..1277)
                     /locus_tag="PKH10_06055"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNT27724.1"
                     /translation="MSRKILPWSIFSVISILWAIEGGGDEFLYLHTASFDFHDGVPFV
                     RVHVADLPAPARLTGAFSAACGSQRSVGDAAEVRADAYGPAQVRYRVAVEELTKGQLA
                     RYRDILEKTSAELGKPVELFSVGGLFSISDRRIDNREYYVTLAGAFDLAAATAAQEKL
                     REDLPARRILVIPVLTAPSKATLSVGGPASRISCDRFVRVTASAPLKVGSFSFPAKSV
                     LFLYASAEGAIGLVAEEDVEKLLYRILPGEMFASAPLETLKAQAVAARSDIFMQLGKR
                     HAADPFHICSEVHCQKLQWGDQKIPQKFVDAVDATRGEILLYRDLYVARAPYSSSCGG
                     HTESNRAVWFSAEQEYLTGVWDADIPTKLDLTRATDVRKFLEGKDGDCGITLNKRFRW
                     ERTFTDQRMDELLASYGVGHILELTPVERGSSGR"
     gene            complement(1243..3561)
                     /locus_tag="PKH10_06060"
     CDS             complement(1243..3561)
                     /locus_tag="PKH10_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNT27725.1"
                     /translation="MRTSFFLFILTVVGPLVAAVPDCLNKEPVTEQCLNEAYEAGEEE
                     SYLEFFPRVSFREKEAANTRRVLALYRTGREADIPVALKQLPDKRRRTLLMQYLSFRA
                     AFSTKRWKDAMITLQKIAVEHPAFYRERRLRCLEGDMLLAQRKWNDAASRYDACLRDG
                     KNESAAFSRLIAKEKGKGPDEALLADYLAFLEEKEHTAQRQQVRDRLLALRRSKGIPT
                     EGTPLFSRWFSILRREGLADEFYCEQITGMGYPVSLEAARYLMDKKRYADAITVMDRE
                     IAKCGTDECRYAYGWEKFRILSWKGAPAEAGNYLIWLANTLSGQRRERALFHAAVSLI
                     EAFDTGTARPLLEETVFKNPQSPFFYQSLYRLGLIYLMEGKEFFAYLLWGNYLFAPQK
                     VKKKGFNAGDAIATMESLTLFYDRLHNYYLFSGANDRECALPEEGDCSVKEFISYYDF
                     LYYHLGDTGKLTREKTPAFSNDQRRSRWRESIRSFDDDPYERLVTAAEKAPIDIRSGE
                     LVENLSFYGRHRILDGLLFYERYARSILSPPGGRKGATGDPAAHFVMSDLALPIYWAA
                     EKHARIIDSIYDAIKDSLRFAPQYGDTENWKILYPVPHIDAVLRLSEEFALSPALIYA
                     IMRAESFYRDWVVSGAGAIGLMQIMPSTFQKIALQGGIKVNDPYNPYESLKAGVWYIS
                     KLLRRFDGNIALAVASYNAGPKRVSQWYSQFGTLPAMSFIELIPYYETRNYVKRVIRF
                     YEIYSYLYEGNFYHLDLGVPFGVKEDPAVVDF"
     gene            complement(3648..>5435)
                     /locus_tag="PKH10_06065"
     CDS             complement(3648..>5435)
                     /locus_tag="PKH10_06065"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNT27726.1"
                     /translation="RNNTVNNLRLWQARAATEFDLASFNSGDYMAAVENKNRSENISK
                     VLYPNDNVLAGKLLRLRQQYFFVSATLQDILYHFKRAQNDLHELPDRIAIHLNDTHPV
                     IAIPELMRILMDQEGLEWDDAWGIVRRTFAYTNHTILPEALEKWDVDIIGPLLPRQLQ
                     IIYEINRRFVNGLRDMGRFDDNAIAQMSIVEDRDPKMVRMANLAIVGSHSVNGVSQLH
                     TDILRNDIFRDFCRWEPELFNNKTNGITPRRWLKKANPFLSDIITARIGDRWVTDLED
                     LRRIEPHAQQAEFRELWHEAKLLAKRHLARHIKEEYGVEVNPAALFDVQIKRLHEYKR
                     QLLNVLHVITLYNRIKADPGREMVPRVVLFGGKAAPGYQVSKLIIKLINSVGRTINND
                     PEVGDRLRVVFLKNYSVSLAEKVIPATDLSEQISTAGYEASGTGNMKFMLNGALTIGT
                     LDGANVEMAEEVGMENIFIFGLRAHEVKDLKKRGYNPREYYDKDPELRQVLDMLNGDR
                     FTPGEPGVFQFLFQNLVLYSDHYCLLADYRSYVETQEKAAALFLDQDEWTRRSILNTA
                     RSGKFSSDRTIRQYADEIWKVSPVIIPDE"
BASE COUNT         1079 a         1596 c         1631 g         1129 t
ORIGIN      
        1 cgccccgaag agccccgctc gaccggtgtc agttcgagta tgtgaccgac gccatacgag
       61 gcaagcagtt catccatccg ctgatcggtg aatgtccgtt cccaccggaa ccgcttattc
      121 aaggtgatac cgcaatcgcc gtcctttcct tcaaggaatt ttcgcacatc ggtcgcccgg
      181 gtcaggtcga gtttggtggg gatatcggcg tcccatacgc cggtgaggta ttcctgctcg
      241 gcagagaacc agaccgctcg attgctttcg gtgtgaccac cgcaggatga ggaatagggt
      301 gcgcgggcca catacaggtc gcgatacagc agtatctcac cccgcgtcgc atcgaccgcg
      361 tcgacgaatt tttgcggtat cttctggtcg ccccactgga gtttctggca gtggacctcc
      421 gaacagatgt ggaacgggtc ggcggcgtgg cgtttgccga gttgcatgaa gatgtcgctg
      481 cgggccgcga ccgcctgcgc cttgagcgtc tccagcggcg ccgatgcgaa catctcgccc
      541 ggcaggatgc ggtacaggag tttctcgaca tcctcctcag ccacgagccc gatcgccccc
      601 tcagcggagg catacaggaa cagaacgctt ttcgccggga acgagaacga tccgaccttc
      661 aacggggccg aagcggtcac tcgcacgaag cgatcgcagg agatgcggga cgcggggcct
      721 ccgaccgaaa gcgtcgcctt cgaaggagcg gtgaggacgg ggatgaccag tatccggcgc
      781 gccggaaggt cctcacggag tttttcctgc gcggcggtcg ccgccgcaag gtcgaacgcc
      841 cccgcgagcg tcacatagta ttcgcggttg tcgatacgac ggtcgctgat ggagaacaga
      901 ccgccgacac tgaacagttc gaccggtttc cccagttctg cggaggtctt ttcgagtatg
      961 tcgcgatagc gcgccaactg tcccttggtc aattcttcga ccgcgacgcg atatcggacc
     1021 tgcgccggac cgtacgcatc ggccctgacc tcggccgcat cgccgaccga ccgctgggaa
     1081 ccgcaggcgg cgctgaacgc tccggtgagc cgcgccggcg ccgggagatc ggcgacatgc
     1141 acccgcacga agggaacgcc gtcatggaaa tcgaacgacg ccgtgtgcag gtagaggaac
     1201 tcgtcgcctc ctccttcgat cgcccacaga atcgaaataa cgctaaaaat cgaccacggc
     1261 aggatcttcc ttgacaccga acggcacccc cagatcaaga tgataaaaat tcccttcata
     1321 caggtaagaa tatatttcgt agaaacggat gaccctcttc acatagtttc gcgtttcgta
     1381 atatgggatg agctctatga acgacatcgc gggaagtgtt ccgaactggc tgtaccactg
     1441 cgagaccctc ttggggccgg cattgtagga ggcgaccgcg agcgcgatgt tcccgtcgaa
     1501 gcgccggagc agttttgaga tataccacac acccgctttg agcgattcat acgggttgta
     1561 gggatcgtta accttgatgc cgccctgcag cgctatcttc tggaaggtgc tcggcatgat
     1621 ctgcatgagt ccgatggccc ccgctccgga cactacccag tcacgataga aactctcggc
     1681 ccgcatgatg gcatagatga gcgccggcga aagcgcgaac tcctcggata ggcgcagtac
     1741 cgcatcgata tgggggaccg ggtaaagtat cttccagttc tcggtgtcgc cgtactgggg
     1801 cgcgaaacgc agggaatcct ttatcgcgtc atagatgctg tcgatgatac gggcatgctt
     1861 ctccgccgcc cagtagatcg ggagggcaag gtcactcatg acgaaatgtg ccgccggatc
     1921 gcccgtcgca cccttccggc cgcccggagg agacaggatg gaacgagcat atcgttcgta
     1981 gaacaagagc ccgtcaagga tgcgatggcg cccgtagaag gaaaggttct cgaccagttc
     2041 gcctgatcgg atgtcgatcg gcgctttctc cgctgcggtg acaagccgct catagggatc
     2101 gtcgtcgaaa ctccggatcg attcgcgcca gcggctgcgc cgctggtcat tcgagaaggc
     2161 cggcgtcttt tcgcgggtca gtttcccggt atctccgaga tgataataga gaaaatcgta
     2221 gtaggatatg aactccttga ccgaacagtc gccctcctcg ggaagcgcgc attcgcggtc
     2281 attcgcgccg ctgaaaagat aatagttgtg cagtcggtcg taaaagagtg tcagcgattc
     2341 catggtggcg atcgcgtcgc cggcgttgaa cccttttttc ttgaccttct gcggcgcgaa
     2401 caggtagtta ccccacagga gataggcgaa gaactccttt ccctccatga gatagataag
     2461 gccaagtctg tagagcgatt ggtaaaaaaa agggctctgc gggttcttga aaaccgtttc
     2521 ctcgagcagg gggcgcgccg tccccgtgtc gaatgcctct atgagcgata ccgcggcgtg
     2581 gaacagtgcc cgctcacgcc gctgccccga aagggtattg gcgagccata tcaggtaatt
     2641 ccccgcctcc gccggtgccc ctttccatga aaggatgcgg aacttctccc acccataggc
     2701 gtaccggcat tcatcagtac cacacttggc gatctcacgg tccatcaccg ttatcgcgtc
     2761 ggcgtaccgt tttttgtcca tcaggtagcg ggccgcctcc agcgaaacgg ggtaccccat
     2821 gccggttatc tgctcgcagt agaattcgtc ggcaagtccc tcgcgtcgaa gtatcgagaa
     2881 ccagcgggaa aagagcggcg ttccttccgt cgggatcccc ttcgaccggc gcaacgcaag
     2941 caggcggtcg cggacctgct ggcgctgggc ggtgtgctcc ttctcctcga gaaaggcgag
     3001 atagtctgcc agaagcgcct catcgggccc tttccccttc tcctttgcta tcaggcggct
     3061 gaaggcggcc gattcgttct tgccgtcgcg gagacaggcg tcgtaccgtg acgcggcatc
     3121 attccacttg cgttgagcga gaagcatgtc tccctcgagg caccggaggc gccgttcccg
     3181 atagaaagcg gggtgctcga ccgcgatctt ctgaagcgtg atcatcgcat ccttccaccg
     3241 cttggtcgaa aaggcggcgc ggaaggaaag atactgcatc aagagcgtgc gtcgccgctt
     3301 atcgggcaac tgcttgagcg ccaccggtat gtcggcctca cgaccggtac ggtagagggc
     3361 gagcacccgt cgggtgttcg ccgcctcctt ttcgcggaac gagacccgcg ggaagaactc
     3421 gagatacgac tcctcctcgc ctgcttcgta tgcctcgttg aggcactgct cggtcaccgg
     3481 ctccttgttg agacaatcgg gaacggcggc caccaacgga ccgaccaccg tgaggatgaa
     3541 taggaaaaag gatgttcgca tcgctccgcc ctcgcttact ccgttgaagt atagcgaacc
     3601 gcgattcgat gtaaaagaaa ttttggattt cgaagcgaat atcttctcta ttcgtcgggg
     3661 atgatgaccg gactgacctt ccatatctcg tcggcatact gacggatagt gcggtcggag
     3721 gagaatttgc cggaccgggc ggtattgagt atcgagcgcc gcgtccactc atcctgatcg
     3781 aggaagagcg ccgccgcctt ttcctgcgtt tcgacatagg agcggtaatc ggcgagcagg
     3841 cagtagtggt cgctgtacag cacgaggttc tggaacagga actggaacac gcccggttcg
     3901 ccgggggtga accggtcgcc gttcaacata tcaagcacct gtcgcaattc cgggtcctta
     3961 tcgtagtatt cgcgcgggtt gtagccccgt ttcttgaggt cctttacctc gtgggcccgg
     4021 agaccgaaga tgaagatgtt ctccatcccc acctcctcgg ccatctcgac attggcaccg
     4081 tcgagcgtgc cgatggtcaa cgcgccgttc agcatgaact tcatgttccc ggtgcccgac
     4141 gcctcgtaac cggcggtcga tatctgctcg gaaagatcgg tggcggggat gaccttctcg
     4201 gcgagcgaca ccgaatagtt cttgaggaag accaccctca gccggtcgcc gacctccggg
     4261 tcgttgttga tcgtccggcc gaccgaattg atgagcttga ttatcagttt cgagacctga
     4321 tagccggggg ccgccttacc gccgaacagc acgacacgcg gcaccatctc gcgaccggga
     4381 tcggccttga tgcggttgta gagcgttatg acatgaagta cattgagcag ttgacgcttg
     4441 tactcgtgga gccgcttgat ctgcacatcg aacagggcgg cggggttcac ctcgaccccg
     4501 tactcctcct tgatatgccg ggcaagatgc cgtttggcga gcagtttcgc ctcgtgccag
     4561 agttcacgga actcggcctg ttgcgcgtgc ggctctatac gccgcagatc ctcgagatcg
     4621 gtcacccatc ggtcccctat ccgcgcagtg atgatatcgg agaggaacgg gttggccttc
     4681 ttgagccagc ggcgcggcgt gatgccgttg gtcttgttgt tgaacagttc cggctcccag
     4741 cggcagaaat cgcggaagat atcgttgcgc agtatgtcgg tatgcaattg cgagacgccg
     4801 ttgaccgaat gggaaccgac gatcgcgaga ttcgccatcc gcaccatctt ggggtcacgg
     4861 tcctcgacga tggacatctg cgctatcgcg ttgtcatcga accgccccat gtcgcggagc
     4921 ccgttgacga accggcggtt tatctcgtag attatctgca gttggcgcgg caggagcggg
     4981 ccgatgatat cgacatccca tttttcgagc gcctcaggca ggatggtgtg gttggtgtag
     5041 gcaaaggtcc gcctgacgat gccccacgca tcgtcccact cgagcccctc ctgatccatc
     5101 aggatgcgca tcagttccgg tatcgcgatg accggatggg tgtcgttgag gtgaatggct
     5161 atccgatcgg gcaattcgtg caggtcgttc tgcgcccgct tgaagtgata gagtatgtcc
     5221 tgcagggtgg ccgagacgaa gaaatactgc tggcgcagac gcaggagttt cccggcgagc
     5281 acattgtcgt tgggatagag cactttggag atattctccg accggttctt gttctcgacc
     5341 gccgccatat aatcgccgct gttaaacgag gcgaggtcga attccgtagc ggcgcgcgcc
     5401 tgccacagtc tcaggttgtt gaccgtgttg ttgcg
//
LOCUS       DAOQUE010000158         5424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16601_length_5424_cov_4.027193, whole genome shotgun sequence.
ACCESSION   DAOQUE010000158 DAOQUE010000000
VERSION     DAOQUE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16601_length_5424_cov_4.027193"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKH10_06070"
     CDS             complement(<1..209)
                     /locus_tag="PKH10_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT27727.1"
                     /translation="MLLLKMAFRNLLRQRRRSLLTGITMLVGYVLFAINTGFSQGAWG
                     MMIDLVTRDHTGHAQIHAKGYLDRPS"
     gene            complement(213..950)
                     /locus_tag="PKH10_06075"
     CDS             complement(213..950)
                     /locus_tag="PKH10_06075"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNT27728.1"
                     /translation="MRKLLFLPLAFFAFTLFAAEPTVEEIVKKSDELYRSATSYAEME
                     MQIETPDWKRNMRLKAWTEGMKKTFITILEPKKDAGIGTLKIDTEMWNYFPKINKVMK
                     IPPSMMMGSWMGSDFTNDDLVKDSTLLDDYTYKLIAGPDATKYYIELTPKAKTATVWG
                     RIVIVANKGDYLPVAQEFYDEKGVKVRVMSFREVKVMGGKKIPTVMELQSLTKPGNKT
                     TIIYKEILFDVLYDPDTFTLRNLQKQR"
     gene            complement(947..1579)
                     /locus_tag="PKH10_06080"
     CDS             complement(947..1579)
                     /locus_tag="PKH10_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT27729.1"
                     /translation="MDFPPKEKRDQRRERGCQTRQRIIDTAAAIIIEEGRPGLTTRAI
                     CEKAGIGKGSLYHHFTDTHELVIEIVNHFMDHYFGAMTAQEFESVDDFFLKFGHQAIA
                     QISTSYKIRERFMPFVVEMVEDEHFIAETMEKNGAMQRALIGKLSALAGGTVPPAVLA
                     DAVLALFTLLEGTESLMQFTRDEKRFLAMWERVAKLLADHVKQNTIRSKK"
     gene            complement(1667..2080)
                     /gene="vsr"
                     /locus_tag="PKH10_06085"
     CDS             complement(1667..2080)
                     /gene="vsr"
                     /locus_tag="PKH10_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch endonuclease Vsr"
                     /protein_id="HNT27730.1"
                     /translation="MVDSLSKEKRSWNMSRVRGKDTRPEIVVRSLLHRSGFRFSLHRP
                     DLPGKPDIVLPKYRTAIFVHGCFWHRHTGCADATTPKTHTDFWTNKFTGNIKRDCKNK
                     KLLKKSGWRVLTVWECETLNPDKLSRKLKRFIETS"
     gene            complement(2081..2914)
                     /locus_tag="PKH10_06090"
     CDS             complement(2081..2914)
                     /locus_tag="PKH10_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNT27731.1"
                     /translation="MRFGKNQEIRFGTTNGAYSLVTDREHYDSQITLTDYEAELVVGH
                     FGANNIHVGRVQLNPQTAAKPFRHYPDGTSIELNLVYPKEEKTELRLYISSKSGFKPE
                     GGEIWFVFLRNGDIWIGSLTESEWRKEAADIKKDENDDLYQNTIYDPDPILTQKIKER
                     EVYVRDRELAIKRMEMAGFSCEYDEEHKLFISRFSRKNYLEAHHLIPIGLQAKFTTAL
                     DSLDNIFCLCPYCHRAVHHAEESKARKILEKLAEKRPVLETYRLDIHDLFNLYAVEEI
                     V"
     gene            complement(2911..4161)
                     /gene="dcm"
                     /locus_tag="PKH10_06095"
     CDS             complement(2911..4161)
                     /gene="dcm"
                     /locus_tag="PKH10_06095"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNT27732.1"
                     /translation="MLKYKELKLALEEFLLEDFPHAEGDEIEAIVTHWLQTPSKKVPF
                     LTASMHEYCVSRVQSLLAESEEERFLIKQPVPAYRFSLPIEWTNIPFPPVQHPSFTFI
                     DLFAGIGGFRIAMQEQGGKCVFSSEWDPHAQKTYEANFGEVPYGDIRKINEKEIPDHD
                     ILCAGFPCQPFSLAGVSARNALHTAHGFACETQGTLFFDIVRIVKEKRPRVLFLENVK
                     NLVGHDEGKTFSVIRNTIESIGYSFSYKVIDASPLVPQKRVRCYMVCLRDGKGPFDFP
                     VISGPPIPLRTILEKEVDDSYTISAKLWQGHKRRTKNNLARGTGFTAFCADLDKPANT
                     LVARYGKDGKECLIPQKSKTPRMLTPRECARLQGFPESFIIAPGKTTAYRQFGNSVAV
                     PVIRLIAEEIATHLRQNKKERTKK"
     gene            4379..4501
                     /locus_tag="PKH10_06100"
                     /pseudo
     CDS             4379..4501
                     /locus_tag="PKH10_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005026533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            complement(4529..4951)
                     /locus_tag="PKH10_06105"
     CDS             complement(4529..4951)
                     /locus_tag="PKH10_06105"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNT27733.1"
                     /translation="MPRKEIVKGSPQEWLTFARSDLKLAKAGKVSGVLLSSLCFHAQQ
                     AAEKALKAVLVLKRKEFPPTHNIKVLVDLLPGSAGFPDKLQQASLLTMYAVNTRYPGD
                     LYHISQKDYAEALSLAGAVVRWAAAEVRAAGAKNGLFS"
     gene            complement(4941..5252)
                     /locus_tag="PKH10_06110"
     CDS             complement(4941..5252)
                     /locus_tag="PKH10_06110"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT27734.1"
                     /translation="MSERLLNTIIRQIVALVAPRRIILFGSAARGDMKKNSDIDLMIV
                     LSDGAAKKKSARILYTALETEGVPFDIVPVTEEDLARHGDTPGLVYRSALREGRVLYA
                     A"
BASE COUNT         1138 a         1417 c         1460 g         1409 t
ORIGIN      
        1 gacggccggt cgaggtagcc cttggcatgt atctgggcgt ggccggtatg gtcgcgggtg
       61 acgagatcga tcatcatccc ccacgccccc tgactgaagc cggtattgat ggcgaagagc
      121 acatagccca ccagcatcgt gatgccggtc agcaggctcc gccgccgctg gcgcaaaaga
      181 ttccggaatg ccattttcag gagtaacatc ggctacctct gcttttgcag gttacgcagg
      241 gtgaaggtgt cggggtcgta tagcacatcg aacagtattt ccttatatat gatggtggtc
      301 ttgttccccg gcttggtgag cgactggagt tccattaccg tcggtatctt cttgccgccc
      361 atcactttga cctcgcggaa actcatcacg cggaccttca cccctttctc gtcgtagaat
      421 tcctgcgcca ccggcaggta gtcgcccttg ttcgcgacga tgacgatgcg cccccagacg
      481 gtggcggtct tcgccttcgg ggtgagttcg atgtagtact tggtcgcgtc gggtcccgcg
      541 atgagtttat aggtataatc gtccaacagc gtcgaatcct tcaccagatc gtcgttggtg
      601 aagtcgctcc ccatccacga acccatcatc atcgagggcg gtatcttcat gactttattg
      661 atcttcggga agtagttcca catctcggtg tcgatcttga gcgtcccgat cccggcgtcc
      721 tttttgggct ccagaatcgt gatgaaggtc ttcttcatcc cctcggtcca cgccttgagc
      781 cgcatgttgc gtttccagtc gggcgtctct atctgcatct ccatttcggc gtaactggtc
      841 gccgagcggt agagttcgtc gctcttcttg acgatctcct ccaccgtcgg ctcggcggcg
      901 aaaagggtga acgcgaagaa ggcgagcggc aggaacaaca gttttctcat ttcttgctcc
      961 gtatggtgtt ctgtttcaca tgatcggcaa gcagtttcgc gacccgttcc cacatcgcga
     1021 ggaaccgttt ctcgtcgcgg gtgaactgca tgaggctctc ggtcccttcg agcagggtga
     1081 agagggcgag caccgcgtcg gcgagcaccg cgggggggac cgtgccgccc gccagcgcgc
     1141 tcagtttgcc gatgagcgct cgttgcatgg cgccattctt ttccatcgtc tcggcgatga
     1201 agtgttcgtc ctcgaccatc tcgacgacga aaggcatgaa gcgttcccgt atcttgtagg
     1261 aggtgctgat ctgggcgatc gcctgatggc cgaatttcag gaagaagtcg tcgacgctct
     1321 cgaactcttg cgcggtcatc gcgccgaagt agtggtccat gaagtggttc acgatctcga
     1381 tgacgagttc gtgggtgtcg gtgaagtggt ggtagagact tcctttgccg atgcccgctt
     1441 tctcgcagat ggcgcgggtg gtgaggccgg ggcggccctc ttcgatgatg atggcggcgg
     1501 cggtatcaat gatgcgttgc cgcgtctggc agccgcgttc ccgtcgttga tctctttttt
     1561 ctttcggtgg aaaatccatc gtttcctcaa ctttaccgag cgcccagtcc agttctttta
     1621 gcagaaatga aaaaggatgt caaggggaaa ttggcaattt atatctttac gaagtctcga
     1681 tgaatctctt tagttttctt gaaagcttat cgggattgag agtttcacat tcccagacgg
     1741 taaggactct ccagccggac tttttcaata gctttttgtt cttgcaatct cttttgatgt
     1801 tgccggtgaa tttattggtc cagaagtcag tatgggtttt cggcgtagtt gcatcagcgc
     1861 atccagtatg ccggtgccaa aagcaaccat gaacaaaaat ggcggttcta tatttcggca
     1921 gaactatgtc gggttttccc ggcaggtcgg gcctgtggag gctgaatcga aagcctgatc
     1981 ggtgcaaaag gctacgaaca actatctccg gcctcgtgtc tttgccgcga acgcggctca
     2041 tgttccagct tcgtttttcc ttgctgagag aatcgaccat tcagacgatt tcttcaacgg
     2101 catacaggtt aaaaaggtca tggatatcaa ggcggtaagt ttccaacacg ggtctttttt
     2161 cggcaagttt ctcgagaatc tttcttgcct tcgactcttc tgcatgatgt accgctcggt
     2221 gacagtaggg gcaaaggcag aagatgttat cgagagaatc cagtgctgtc gtgaatttcg
     2281 cctgtagtcc gatggggatc aaatgatggg cctcgaggta gttctttctg ctgaagcggg
     2341 aaatgaaaag tttgtgttcc tcgtcatatt cgcaggaaaa accagccatc tccatccgtt
     2401 ttatcgccag ctctctgtct cttacatata cttcacgctc ttttattttc tgtgtaagta
     2461 tggggtcggg atcatatatt gtattctgat agagatcgtc attctcatct ttcttgatat
     2521 cggccgcttc ttttcgccac tctgattcag tcagggaacc aatccagata tcgccgtttc
     2581 tcagaaagac aaaccagatt tcgcctccct ccggtttaaa tcccgatttg gatgaaatgt
     2641 aaagtcgaag ttccgttttc tcctctttgg gatagacgag attgagttcg atagaggttc
     2701 catcagggta gtgacgaaaa ggctttgcgg ctgtttgcgg gtttaattga actctgccga
     2761 catggatgtt gtttgcgccg aaatgtccaa cgaccagttc ggcttcataa tcagttaaag
     2821 tgatttggga atcgtagtgt tcccggtcgg ttaccaatga ataagcacca ttggtcgttc
     2881 cgaacctgat ttcctgattt ttcccgaatc tcattttttt gtcctctctt tcttgttttg
     2941 ccggagatgc gtggctattt cctccgctat caggcggata accgggacgg caacagaatt
     3001 accgaactgg cgataagcag tcgttttccc gggagcgata atgaacgact cggggaagcc
     3061 ttgaagtctg gcgcattccc gaggggtgag catcctcggg gttttcgact tctgcggtat
     3121 cagacactcc ttcccgtcct tgccgtacct tgcgacaagc gtgttggccg gtttgtccag
     3181 atcggcgcag aatgcagtaa agccggtacc acgcgcaaga ttgttttttg tgcgtcgttt
     3241 atgtccctgc cagagttttg ccgagattgt gtacgagtca tcaacctctt tttcaagtat
     3301 agtgcgaagc ggtatcggcg ggccggaaat aacaggaaag tcgaatggcc ctttgccgtc
     3361 gcgtagacaa accatataac aacggacgcg cttctgggga actaagggac tggcatcaat
     3421 cactttgtaa gaaaagctgt aaccgatgga ttcgatggtg ttgcgaatga cggaaaatgt
     3481 cttgccttcg tcgtgtccaa caaggttttt cacattctca aggaatagca cccgggggcg
     3541 tttttctttg acgattctta ctatgtcaaa aaacaaggtt ccctgcgtct cgcaggcgaa
     3601 cccatgggca gtgtggaggg cgttgcgcgc agacacaccg gcaaggctga acggctgaca
     3661 ggggaatccg gcgcagagaa tatcatggtc gggaatctct ttctcattaa ttttgcgaat
     3721 gtcgccgtat ggcacttcgc cgaagttggc ttcgtatgtt ttctgtgcgt gaggatccca
     3781 ctcacttgaa aacacgcatt tcccgccctg ttcctgcatg gctattctga atccgcctat
     3841 cccggcgaac agatcgatga aggtgaagga tgggtgctgg acggggggga aggggatatt
     3901 tgtccactcg atgggcaggg aaaatcgata tgccggcaca ggctgtttta tgagaaaacg
     3961 ctcttcctcg ctttccgcaa gaagagactg gacgcgggaa acgcaatact catgcatcga
     4021 cgctgtcagg aacggaacct tttttgacgg cgtttgcaac cagtgggtga ctatggcttc
     4081 aatctcatct ccctcggcat gaggaaaatc ctccagaaga aactcttcga gcgccaattt
     4141 caactctttg tatttcagca tcggactctc cattctgttc aatctcgtct tggcattttc
     4201 tcgatgcctc tgaaagatac tttttgcgag gttgagaatc aagaattttc cttgattcga
     4261 gaaatctcca accgttggtt agagaaatca gttgagcaga ctcccagact tgcatttctt
     4321 caccgtcgcg ctaaagttcc cttgtctttt tgaaggataa gtaaggaggt tttctgcaat
     4381 ggacatcccg cggatattca acatcactga aagcgcccac cgcatccaca accccttcac
     4441 ccccgacaag tacgccaccc tcggcgccgc cctgcgcatg gaacggggga cccgcatcct
     4501 cgtgaaacgg tgaagtgaga cggaaaatct aggagaaaag accgttcttc gcgccggccg
     4561 cgcgaacctc cgcggcggcc catctgacga ccgcgccggc cagtgacaac gcctcggcgt
     4621 aatccttctg cgatatgtga tagagatcgc ccggataccg cgtattcacc gcatacatcg
     4681 tgagaaggga tgcctgttga agtttatcgg gaaacccggc cgaaccgggc agaagatcga
     4741 ccagaacctt gatgttgtgc gtcggcggga actccttccg tttcagcacc agcaccgcct
     4801 tgagcgcctt ttcggccgcc tgctgtgcgt ggaaacaaag cgaggaaaga aggacgccgc
     4861 ttaccttgcc cgcctttgcg agtttgaggt cgcttctggc gaaagtaagc cactcctgcg
     4921 gagagccctt tacgatctct ttacgcggca tagagcaccc gtccctcgcg gagtgcggag
     4981 cgatacacca ggccgggggt gtcgccgtga cgcgccaagt cctcttcggt caccggcacg
     5041 atgtcgaacg gaacgccttc cgtttccagc gcggtgtaga ggatacgcgc gcttttcttt
     5101 tttgcagcgc cgtcggaaag gacgatcatc aggtcgatgt cgctgttctt cttcatatcg
     5161 ccgcgggccg cgctgccgaa caggatgatg cggcgcggcg cgaccagcgc cacgatctgg
     5221 cgaatgatgg tgttgagaag ccgttcactc actttcatgc cgccagtatg cccgataacg
     5281 gacaaaaaag caagcgcaac atagaactgc caccactggt tgcaaaaatc agttgagtcg
     5341 atatccgcca cttgcatttc ttcgccgttg cgctaaagtc ctcttgtctt tttgtcggat
     5401 aaacaaggag ctcctctgcc atgg
//
LOCUS       DAOQUE010000159         5416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16631_length_5416_cov_4.462227, whole genome shotgun sequence.
ACCESSION   DAOQUE010000159 DAOQUE010000000
VERSION     DAOQUE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16631_length_5416_cov_4.462227"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /gene="map"
                     /locus_tag="PKH10_06115"
     CDS             complement(<1..561)
                     /gene="map"
                     /locus_tag="PKH10_06115"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNT27735.1"
                     /translation="MRKGEIIIKTPEQIAGIARAGVLTGRLFDELRSYIKPGVTTLQI
                     DAFADRFIKKNGGKPTFKSVENYHHATCVSLNNEVVHAIPRKDRVVQRGDIVKVDCGV
                     TLDGFIGDRTMTYLMPGVSDEARRLVEVTRECLNIGIGQMLVGNRIGDIGAAIQEYAE
                     SHGYAVVKEYVGHGTGIHLHEEPSVPH"
     gene            complement(562..2952)
                     /gene="pheT"
                     /locus_tag="PKH10_06120"
     CDS             complement(562..2952)
                     /gene="pheT"
                     /locus_tag="PKH10_06120"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNT27736.1"
                     /translation="MLLSLSWIREYVDLEGITPEDIVKRLALSTAEIESAHWVGRHFE
                     QVKAARIEKIEKHPNAEKIRLATIFDGKDRATVVCGAPNIEEGQVVPYAPLGTVLPGD
                     FEIKPAKIRGVESCGMLCSAKELGLGDDHSGILQLDRKVELGTPLSTLYGPADLIIEI
                     ENKTVNHRPDLWGHYGYARELRTIFNRPWKKKLEFTGVKPDKKEEELSIEFATDKALH
                     YIGVKMSGIKIGPSPDWLRARIEAIGHRSINNIVDITNFVMFETGHPLHAFDRRDIAG
                     STIIVRNASAGEKFTTLDGVERVLLERDIVIADKDKAVALGGVMGGLHSEVESDTTEI
                     IIESALFDPATIRRTANRLDLRTDAAQRFEKAMWVENSWLAVQRCIELIKELVPGAVV
                     TSEAAHRDASGKYGFTGSISTSCDRIRAMLGVRRDDLPDARIEGILLLLDFEVKREGD
                     RLVIPVPPHRRSKDVSLEADIVEEVGRIHGYDNIMPRAPLFPMTPPPRNGLLDKEERL
                     RDVLVREFGACEIMTYSFLSDNEHAAFPLPDNEAVRAPSLKETPWLRHTLTPGMLKAL
                     AVNHRQYERFTLFEFGRVFRRGGENRRLAVLIRDDRDIFRMTKRIVEAFMAELKVPAF
                     QFERAKKESVPFHEDVALHPGRGALVRAMGKLVGMCGEVHPALLEQWDVRGRAGIMEF
                     DIDLLGSLPAKSEKYKPIPRFPATFFELTVLTSERTESAAVGTVAYKTIDKKIFSGCE
                     VVSSYKGPGVPEGQMSLSLRVTLAAPDRTLTSDEMKQAQEALIAAFAKQGWPLKGA"
     gene            complement(2964..3980)
                     /gene="pheS"
                     /locus_tag="PKH10_06125"
     CDS             complement(2964..3980)
                     /gene="pheS"
                     /locus_tag="PKH10_06125"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNT27737.1"
                     /translation="MKKRIEKMRGEFTTALQAAAVPEDAEKVRVAFLGKKGVLTEVLK
                     GMKDLTPDERREVGALSNALKDEFERLLAARAAELGRGEIEKKLAAEWEDISWPSGLS
                     RGAAHPIAVIRRRVKEIFVSMGFEVLDGPEMEEETNNFDKLNIPPDHPARDAQDTFWL
                     DNGMLLRTHTSPVQVRGMLSHTPPFKFIAPGKVYRNEAVDASHEHTFHQIEGMVVDKN
                     ISVAHLIYSMKTLLTGIFEKEVEVRLRPGFFPFVEPGFELDIKCLICGGKGCSVCKQS
                     GWLELMPCGMVHPVVLRNGGIDPEEFSGFAFGLGLERLVMMKYGIDDIRLFHGADLRF
                     FTQF"
     gene            4190..4483
                     /locus_tag="PKH10_06130"
     CDS             4190..4483
                     /locus_tag="PKH10_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27738.1"
                     /translation="MRNAFFNGVFLFFFSLLFSTEFRAIVQTDGKLWYAVFLSLSFSQ
                     EGMDAERLDPELLQVSDPYLSIREAMYIRNRLEEILDDDEEDEDAWEENGDEV"
     gene            4452..5141
                     /locus_tag="PKH10_06135"
     CDS             4452..5141
                     /locus_tag="PKH10_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27739.1"
                     /translation="MHGKRTVMRCSVCAIILLTALSLGATDPGLEQMTRYLKGKRISV
                     ETLEKDYSCHPLVDALAKGLQQVKDLEKAVRDARDNTRVAALFPRMSAWAKYRSDDRL
                     YLYRQNNISVGKDYVTVGPDDNNTTIGDINAFEVGGKLEFDLARLLYNTDLIKFSEQE
                     QRLHQFRTELVEKMTYAYYYVAVVKALTLNGVETPPEVLLPAEIAAKKLAVWFYDLTG
                     LRLDRCAATQP"
     gene            5120..>5416
                     /locus_tag="PKH10_06140"
     CDS             5120..>5416
                     /locus_tag="PKH10_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27740.1"
                     /translation="MRRHTALIALALLCACGRFSMPDSEYLYFEIVSPPEDRVWYPEE
                     TMTIVFNEEIASDTVAGAVTLSYEGDPAHGTTVTASGDTLKIAGLLPDATITVTA"
BASE COUNT         1016 a         1738 c         1566 g         1096 t
ORIGIN      
        1 atggggcacc gacggctcct cgtgcaggtg gatgccggta ccatgaccga catactcctt
       61 gacgaccgcg tagccgtgcg attcggcgta ctcctgtatc gccgcaccga tgtcgccgat
      121 gcggttgccg acgagcatct gtccgatgcc gatattaaga cattctcgcg tcacctcgac
      181 gagccgccgc gcctcgtcgc tcacccccgg catcaggtag gtcatcgtcc ggtcgccgat
      241 gaaaccgtcg agcgtcacgc cgcagtcgac cttgacgata tcaccgcgct gaacgacgcg
      301 atccttgcgc gggatggcgt ggaccacctc gttattgagc gagacgcagg tggcatgatg
      361 gtaattctcc accgacttaa aggtcggttt gccgccgttc tttttgatga accggtcggc
      421 gaaggcatct atctggagcg tcgtcacccc tggcttgata taacttctca gttcgtcgaa
      481 gagtcgcccg gtcaacaccc ccgcccgcgc gataccggct atctgctccg gcgtctttat
      541 gatgatctcg cccttgcgca ttcaggcccc tttgagcggc cagccctgtt tcgcgaacgc
      601 cgcgatgagt gcctcctgcg cctgcttcat ctcgtcgctc gtgagcgtcc ggtcgggcgc
      661 agcgagcgtc acgcgaagcg aaaggctcat ctgcccctcg ggaacacccg gccccttgta
      721 ggaactcacg acctcgcacc cggagaatat cttcttgtcg atcgttttat aggccaccgt
      781 gccgaccgcc gccgattcgg tccgttccga cgtcagcacg gtgagttcaa agaaggtcgc
      841 ggggaatctc ggtatcggct tgtacttctc gctcttggcc ggcagggaac cgagcaggtc
      901 gatatcgaac tccatgatgc cggcgcgccc cctgacatcc cattgttcga gcagggcggg
      961 atgcacctcg ccgcacatgc ccaccagttt tcccatcgcc cggaccagcg cgccacgccc
     1021 cgggtgaagc gccacatcct catggaaggg caccgattcc tttttcgccc gttcgaactg
     1081 gaacgcgggg accttcagtt cggccatgaa cgcctcgacg atccgcttgg tcatccggaa
     1141 gatgtctcta tcgtcgcgga tgagcaccgc caaccggcgg ttctcgccgc cgcgacggaa
     1201 cacccgaccg aactcgaaca gcgtgaagcg ttcgtactgg cgatgattca ccgccagcgc
     1261 cttcagcatc cccggcgtca gcgtatgccg caaccacggg gtctccttga gcgacggcgc
     1321 gcgcaccgcc tcgttgtcgg gcaaagggaa cgccgcatgc tcgttgtcgg agaggaagga
     1381 gtaggtcatg atctcgcagg cgccgaattc gcgcaccaat acatcgcgga gccgctcctc
     1441 cttatcgagc agtccgttgc gcggcggcgg tgtcatcggg aacagcggcg cgcgcggcat
     1501 gatgttgtcg tacccgtgga tgcgccccac ctcttcaacg atatcggcct caagtgacac
     1561 atccttactg cgacggtgcg gcggcaccgg gatgacgagc cggtcccctt cccgcttcac
     1621 ctcgaaatcg aggagcagga gtatcccctc gatccgtgcg tcgggtagat cgtcgcggcg
     1681 cacgccgagc atggcccgga tacggtcgca tgaggtcgat atcgaaccgg tgaagccgta
     1741 cttcccgctc gcatcgcgat gggccgcctc gctcgtcacg accgcaccgg ggacgagttc
     1801 ctttatcagt tcgatgcacc gctggacggc aagccacgaa ttctcgaccc acatcgcctt
     1861 ctcgaaccgc tgggccgcgt cggtacgcag gtcgagccgg ttggcggtgc ggcgtatcgt
     1921 tgccggatcg aagagggccg attcgatgat gatctcggtc gtatcagact cgacctcgct
     1981 gtggaggccg cccataacgc caccaagcgc gaccgccttg tccttatcgg cgataacgat
     2041 atcgcgttcg agcagtacgc gctcgacgcc gtcgagcgtc gtgaacttct cgcccgccga
     2101 cgcattccgc acgataatgg tcgatccggc tatgtccctg cggtcgaagg cgtggagcgg
     2161 gtgtcccgtt tcgaacatca cgaagttggt gatgtccacg atgttgttta tggagcggtg
     2221 accgatcgcc tcgatacggg cgcgcaacca gtcgggcgac ggaccgatct tgatacccga
     2281 catcttgacg ccgatgtagt ggagcgcctt atcggtagcg aactcgatgg aaagttcttc
     2341 ctctttttta tcgggcttga cgccggtgaa ctcgagtttc tttttccacg gccggttgaa
     2401 tatcgtccgc agttcgcggg cgtaaccgta gtgaccccag aggtcgggcc gatggttgac
     2461 cgtcttgttc tctatctcga tgatgagatc ggccggaccg tagagcgtcg agagcggcgt
     2521 accgagctcc accttccggt cgagttgcag gatgcccgaa tgatcgtcgc caagccccag
     2581 ttccttcgcc gaacagagca tgccgcaact ctcgacgccg cgtatcttgg ccggctttat
     2641 ctcaaagtcc ccggggagca ccgtgccgag cggcgcgtag ggaacgacct gcccctcctc
     2701 gatgttcggg gcgccgcaga ccaccgtcgc gcgatccttt ccgtcgaata tcgtcgcgag
     2761 tcgtatcttc tcggcgttcg gatgcttctc tatcttctcg atgcgggcgg ccttgacctg
     2821 ctcgaagtga cgaccgaccc agtgggccga ctctatctcg gcggtcgaga gcgcgagtct
     2881 tttcacgata tcctcgggcg ttatcccctc aagatcgaca tattcccgta tccatgaaag
     2941 cgacagcaac atcgtagcct cctttagaac tgcgtgaaga aacggagatc ggcgccgtgg
     3001 aagaggcgga tgtcgtcgat cccgtacttc atcatgacca gtcgttcgag cccgagcccg
     3061 aaggcgaacc cggagaactc ctcagggtcg atgccgccgt tacgcaggac cacggggtgg
     3121 accataccgc acggcatcag ttcgagccaa ccgctttgtt tgcagaccga acaacccttg
     3181 ccgccgcaga tgaggcattt gatgtcgagt tcgaaccccg gctcaacgaa cgggaagaac
     3241 ccggggcgca ggcgcacctc gacctctttt tcgaagatac cggtcagcag ggtcttcatg
     3301 gagtatatca gatgggcgac cgatatgttc ttgtccacca ccatcccttc gatctggtgg
     3361 aaggtgtgct cgtggcttgc gtcgaccgcc tcgttacgat agaccttgcc gggggcgatg
     3421 aacttgaacg gcggggtgtg cgacagcatg ccgcgcacct gcaccggcga ggtatgggtc
     3481 cgcaggagca tcccgttgtc gagccagaag gtgtcctgcg cgtcgcgcgc cggatggtcg
     3541 ggcgggatat tcaacttatc gaagttattt gtctcctcct ccatctcggg accgtcgagc
     3601 acctcgaagc ccatcgagac gaatatctcc ttgacgcgcc gacggatgac ggcgatcggg
     3661 tgcgccgcgc cgcgtgaaag cccactcggc cacgagatat cctcccactc ggcggccagt
     3721 ttcttctcta tctcgccgcg accgagttcg gccgcccgcg ccgcaagcag cctctcgaac
     3781 tcatccttga gcgcgttcga gagcgctccg acctcgcgcc gctcgtcagg ggtaagatcc
     3841 ttcatccctt tgagtacctc ggtcagcacc cctttcttgc cgaggaacgc cacgcgcacc
     3901 ttctcggcat cttcgggcac cgcggccgcc tgcaacgcgg tcgtgaactc gccgcgcatc
     3961 ttctcgatcc tcttcttcat ctcggactcc gtcgcataga ccgacaaaac gccgtaacga
     4021 taccacagtc gcgcgatatt tcaaacaaat aacccaagaa ctttttcacg gtaatgccgg
     4081 gggggcggga ctcgttcaca aataatttct tttcttcggc gttttttttc ttgacttcat
     4141 ttttttcgtc aaagtagtat cgcgccggtc gtccgggttg tgggatacta tgcggaacgc
     4201 tttcttcaac ggcgtatttc tttttttctt ctcgctactt ttctccaccg agttccgcgc
     4261 catcgtacag accgacggca aactctggta cgcggtcttt ctctcgctct cgttctcgca
     4321 ggaaggcatg gatgccgagc ggctggaccc cgaactattg caggtcagcg acccctatct
     4381 ttcgatacgc gaggcgatgt acatcaggaa ccggttggaa gagatactcg atgatgacga
     4441 ggaggatgaa gatgcatggg aagagaacgg tgatgaggtg tagcgtctgc gcgatcatcc
     4501 tgcttaccgc cctgtcgctc ggcgcgaccg atccggggct cgagcagatg accagatacc
     4561 tcaaggggaa gaggatctcc gtcgagacgc tcgagaagga ctacagttgc cacccgctcg
     4621 tcgatgccct ggccaagggg ctccagcagg taaaggacct cgaaaaggcg gtgcgcgacg
     4681 cacgcgacaa cacgcgggtc gccgcgctgt tccccagaat gtcggcgtgg gcgaaatacc
     4741 ggagcgacga ccgactctac ctgtaccggc agaacaacat cagcgtcggc aaggattatg
     4801 tcaccgtcgg tcccgacgac aacaacacga ccatcggcga catcaacgcc ttcgaggtgg
     4861 gcggtaaact cgagttcgat ctcgcccggc tcctgtacaa caccgacctc atcaagttca
     4921 gcgaacagga acagcgcctg caccagttcc gcaccgaact ggtcgagaag atgacctacg
     4981 cctactacta tgtcgcggtc gtgaaagcgc tgacgctgaa cggcgtcgag acgccgccgg
     5041 aggtgttgct gcccgccgag atagcggcga agaaactggc ggtatggttc tacgatctca
     5101 ccggactgag gctcgaccga tgcgccgcca cacagccctg atagcgctcg ccctcctctg
     5161 cgcctgcgga cgcttctcga tgcccgacag cgagtacctc tatttcgaga tcgtgtcgcc
     5221 gcccgaagat cgggtatggt acccggaaga gacgatgacc atcgtcttca acgaggagat
     5281 agcgtccgac accgtcgccg gcgcggtgac cctgtcttac gaaggcgacc cggcccacgg
     5341 gaccaccgtg accgcctccg gcgacaccct caagatagcc ggtctcctgc ccgacgcgac
     5401 gatcaccgtc accgcg
//
LOCUS       DAOQUE010000160         5405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16684_length_5405_cov_3.768037, whole genome shotgun sequence.
ACCESSION   DAOQUE010000160 DAOQUE010000000
VERSION     DAOQUE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16684_length_5405_cov_3.768037"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKH10_06145"
     CDS             <1..295
                     /locus_tag="PKH10_06145"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341719.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HNT27741.1"
                     /translation="AAAHHLVRVEVETATLAEVKAALAARADIIMLDNMDQTTIRKAI
                     ALIGGRAVTELSGGVTLEKIPALSRLGADLISVGALTHSAPSVDINLTLTTGK"
     gene            295..1203
                     /locus_tag="PKH10_06150"
     CDS             295..1203
                     /locus_tag="PKH10_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27742.1"
                     /translation="MLSPRLSIAFFALFLAAVLVTGIFWIFLSDRHGFTAADEPLIAE
                     VLNREFRPGDIIFPKPDWDIGFTRYLRDGITDISYTLDAFTKEELSDLNNNGVNVLWF
                     VVDSEERWGDIRDRFALKEQGRYPAGEAFVIKGQPSGDAVLKLFDFTDDLAQAREVYL
                     TDARGGREACSWERDRWKCGRQEWQYVGPQRTLMAGRWQKANWAHPRTERTLHVVFEN
                     TVGASVLVIGSSFLERAYREENGKPVRVTVTVDGRELLKYDNENIKKLYRHRIALPEG
                     AKMIDLSFWVKFDGARHFVFNGYLGT"
     gene            1200..2867
                     /locus_tag="PKH10_06155"
     CDS             1200..2867
                     /locus_tag="PKH10_06155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT27743.1"
                     /translation="MTLHRFDKWDWVLSLALALVAALAVMLTSHLVGLVRDEGFYMRT
                     AETTADYIQFSLDSIGRGQFAEPFSKRTITRFFEFNSEHPTFVKNLFGLSHFIFYKKL
                     KLLSFVDSIRLVAALFAAATAFLIYLFGRRFFSRAVAIAAPPLFFTMPHIFFHSHLAC
                     FDVPILFFWTATFILYAIYLVERRTGLAFLIAVLFGFAMATKHNVYFIPPLLALLWLA
                     MHVLHYRSLEPRHKGFVGFFRAVPLPLWLMPTVSLPVFFLSWPWLWYDTVKRFGNYLA
                     FHTYHVNYTNYYFGQELSVAPFPFSYPWGMTFFTTPVPQLVCFFAAIALFLCWMFGRG
                     RTPVERHIGALLLIGALFPIFLIALPSVPIFGGIKHFFTGQPILLTAGLAVVLGGAAE
                     LFRRWPKVKEQAVQGMVVAAALFVLVIPNAKFVMHGAGFFNALIGGAQGAAEHYMQRN
                     FWGYDILPLCPVLNAVAPRGAKVYVMSYAEGLNWNSFLYLKREGHIRNDIQGTNELRD
                     ADFAFFFYEKQNESVLYDLYREFGTVRPLELSETDSVYFSALFGRVQ"
     gene            2864..3934
                     /locus_tag="PKH10_06160"
     CDS             2864..3934
                     /locus_tag="PKH10_06160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNT27744.1"
                     /translation="MTYLFTNPQVKRWKTLFFISFLFNCFLVVALYVEAGEEEQDRLS
                     DPPADTIGVVAPQAESLPAKERSAGPVVGEPIPVWLTITENFYNAFTADKDIARHAEH
                     FDFPRLAEILSVYVSRVLMWDLILHKDVMKGDTLSFVFRMVPAEEMKQREDMPDPVEI
                     LAVSYFSNRYNKFIDLFSYYAASKKRRNFYCADGRLLERVLKNPPIRDHVAVITLFDE
                     YPPKHEGVDFKAPTGTPVYATFDGRITRADWQTRFNGYSLELTAKNGALVAKYLHLAE
                     RMADEGQEIKAGEIIAKSGSTGKAAVPHLHYQINAGIRGKALDPFKHHETYFEYLKGD
                     ELRKFQETVVDYKRILKKVVGG"
     gene            4056..>5405
                     /locus_tag="PKH10_06165"
     CDS             4056..>5405
                     /locus_tag="PKH10_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27745.1"
                     /translation="MKRSCFLTASLVLLMASSLLLAQEQMIVMPIAAKKTNERKMADD
                     VFENFTMLFSSIKGVQAVSYEKELSPKDAARCKDDLKCMLKVAKEADAAYYLLSKISS
                     DSIMVYLMSGAGKKLGTEDVEYSKDADEEDIAAKMLAATKKLVDKYTGSGSSSSSSSA
                     RSSRGSTSKTYTYSEVKEEIKRGMDFYRKGDLSGAEESFYRAANDMNCKCSQNDDAKK
                     HYENIVNIKKGLSAADDSVSSQDYGKAERILKTILDLDTQVREAGYKLMSFKKDNVER
                     VIYRKPNSDDVAAVDSIHNQFKRKIEDVRKWRLEQQASVDKWVNDRIKAREAALAERE
                     KKLQDFVAMSKEAEKALKEKVQGLKYQWEKDDSSLEQEIVNLESQITMIEQREKGVVK
                     AANKGRENAQKKEYEALDKKNDEIRKKIQAEKEAYYNKQKEWEDAEGKKVTARIADIE
                     "
BASE COUNT         1185 a         1576 c         1533 g         1111 t
ORIGIN      
        1 cgccgcggcg caccatctgg tccgggtcga ggtcgagacc gccaccctcg ccgaggtgaa
       61 ggcggcgctt gcggcccgcg ccgacatcat catgctcgac aacatggatc agacgacgat
      121 acggaaagcg atcgctctca tcgggggacg cgccgtgacc gaactgtcgg gcggcgtgac
      181 gctcgaaaag ataccggccc tctcgcggct cggcgccgac ctcatatcgg tcggggcgct
      241 gacccattcg gccccgtcgg tcgacatcaa tctgaccctc accaccggga aataatgctg
      301 tcacctcgtc tttccatcgc gttcttcgcg ctgttcctcg cggcggtact cgtgaccggc
      361 atcttctgga tcttcctttc cgaccggcat ggattcacgg cggccgacga gccgctcatc
      421 gccgaggtgc tcaaccgcga gttccggccg ggagacatca tcttcccgaa acccgactgg
      481 gacataggct tcacccgata cctgcgcgac ggcatcaccg acatatccta cacgctcgac
      541 gccttcacca aggaggaact gagcgatctc aacaacaacg gcgtcaatgt cctgtggttc
      601 gtggtcgact ccgaagagcg ctggggagac atccgcgacc ggttcgcgct caaggaacag
      661 gggcgctatc cggcgggaga ggcattcgtc atcaagggac agccttcggg tgacgcagtg
      721 ttgaaactct tcgatttcac cgacgacctc gcgcaggcga gggaggtgta cctgaccgat
      781 gcccggggag gtcgagaggc gtgctcgtgg gagcgcgacc gctggaaatg cgggcggcag
      841 gaatggcagt atgtcgggcc ccagcggacg ctcatggcgg gccgctggca gaaggcgaac
      901 tgggcccatc cgcgcaccga gcggacgctc catgtggtct tcgagaacac cgtcggggcg
      961 tcggtgctgg tcatcggctc ctccttcctc gaacgggcct accgcgaaga gaacggcaag
     1021 ccggtccgcg tcaccgtcac cgtcgatggc cgggaactcc tgaaatacga caatgagaac
     1081 atcaagaaac tgtaccgcca ccgcatcgcg ctcccggaag gggcgaagat gatcgacctc
     1141 tcattctggg tgaagttcga cggggcgcgt catttcgttt ttaacggata cctcggcaca
     1201 tgacactgca tcgtttcgac aagtgggact gggtcctgtc gctcgcgctc gctttggttg
     1261 cggcgctcgc cgtgatgctc acctcgcatc tggtggggct cgtccgtgac gaagggttct
     1321 atatgcggac ggcggagacg accgccgatt acatccagtt ctcgctcgac agcatcggtc
     1381 gtggtcagtt cgcagaaccg ttcagcaaac ggaccatcac ccgctttttc gaattcaaca
     1441 gcgaacatcc aaccttcgtc aagaacctgt tcggcctgtc gcacttcatc ttctacaaaa
     1501 aactcaaact gctctcgttc gtggactcga tacggctcgt ggcggccctt ttcgccgcgg
     1561 cgaccgcctt tctcatttac cttttcggcc gccgcttctt ctcgcgggcc gtcgccatcg
     1621 ccgcgccgcc gctcttcttc accatgccgc acatcttctt ccacagccat ctcgcctgct
     1681 tcgacgtgcc gatcctcttc ttctggacgg ccaccttcat cctctacgcg atctacctcg
     1741 tcgagaggcg gaccggactc gccttcctca tcgcggtcct cttcggtttc gcgatggcga
     1801 ccaagcacaa cgtctacttc atcccgccgc tgctggcgct tctgtggctc gcaatgcatg
     1861 tccttcatta ccgctccctc gagccgcgcc acaagggttt cgtcgggttc ttccgcgccg
     1921 tgccgctacc gctctggctc atgccgacgg tatcgctgcc ggtcttcttc ctttcgtggc
     1981 cgtggctgtg gtacgacacc gtcaaacggt tcggcaacta tctcgcgttc cacacctatc
     2041 atgtcaacta taccaattac tacttcgggc aggaactctc ggtcgcgccg ttccccttct
     2101 cctacccgtg ggggatgacc ttcttcacca ccccggtacc gcaactggtc tgctttttcg
     2161 ccgctatcgc gctgttcctc tgctggatgt tcggtcgcgg ccgaacgccg gtcgaacgtc
     2221 atatcggcgc gcttctcctt atcggcgcgt tgttccccat cttcctgata gcccttccaa
     2281 gcgtcccgat attcggcggc atcaaacatt tcttcaccgg tcaaccgatc ctgctcaccg
     2341 cggggctcgc ggtggtgctc ggtggcgcgg cggaactatt ccgccggtgg ccgaaggtga
     2401 aggagcaggc ggtgcagggg atggtcgtcg cggcggcact gttcgtcctc gtcatcccca
     2461 acgcgaaatt cgtcatgcac ggcgccggct tcttcaatgc cctcatcggc ggcgcgcagg
     2521 gtgcggccga acactatatg cagcgcaatt tctgggggta cgacatcctg ccgctctgcc
     2581 cggtgctcaa cgccgtcgcg ccgcgcggcg ccaaggtcta tgtgatgagt tacgccgaag
     2641 ggctcaactg gaactcgttc ctctacctca agcgggaggg gcacatccgg aacgacatac
     2701 aggggaccaa cgaactgcgc gacgccgatt tcgcgttttt tttctatgaa aaacagaacg
     2761 aaagcgtact atacgacctg taccgcgaat tcgggacggt gcgaccgctc gaactctcgg
     2821 agaccgattc ggtctatttc tcggctcttt tcgggagggt gcagtgacct atctgttcac
     2881 caatcctcag gtcaaacgct ggaagacact ctttttcata tcgttcctct tcaattgctt
     2941 cctcgtggtc gcgctctatg tcgaggccgg cgaagaagaa caggatcggc tgtccgatcc
     3001 cccggcggat acgatcggtg tcgttgctcc acaggccgag tcgttgcctg cgaaagagcg
     3061 tagtgcgggg ccggtcgtcg gcgagccgat accggtctgg ctcaccatca ctgagaattt
     3121 ctacaatgcc ttcacggccg acaaggatat cgcgcgccac gcggagcatt tcgattttcc
     3181 gcgactcgcc gagatccttt cggtctatgt cagccgcgtg ctcatgtggg atctgatcct
     3241 gcataaggat gtcatgaagg gtgataccct ctcgttcgtg ttccgcatgg tccccgccga
     3301 agaaatgaaa caacgcgagg acatgcctga tccggtcgag atactggcgg tcagttattt
     3361 ctcgaaccgc tacaacaagt tcatagacct tttctcctat tatgcggcat cgaagaaacg
     3421 caggaacttc tactgtgcgg atggtcgcct gttggagcgg gtcttaaaga acccgccgat
     3481 tcgcgaccat gtcgccgtca taaccctgtt cgacgagtat cccccgaaac acgagggtgt
     3541 tgacttcaag gctcctaccg gtacgccggt ctacgccacc tttgacggtc gtatcacccg
     3601 cgccgattgg cagacccgct ttaatggcta ttcgctggag ctcaccgcca agaacggcgc
     3661 gctggtcgcc aagtatctac atcttgccga gcggatggcc gacgaaggac aggagattaa
     3721 agcgggagag ataatcgcca aaagcggtag caccggaaag gcggcggtgc cccatttgca
     3781 ctaccagatc aacgcaggca ttcgcggcaa ggcgttggac cccttcaaac accacgaaac
     3841 ctatttcgag tatttgaaag gtgatgaatt gagaaaattc caagaaacgg tagtggatta
     3901 caaaagaata cttaaaaaag tggttggtgg ttgacgatat tatgtataaa aagtttgact
     3961 ttttgccatt cgatggatat cctgctcgac gaagaggcaa tgaagattga aaagatgact
     4021 agttttaatg agataagttg ctcaaggagg gtctcgtgaa aagatcatgc ttccttaccg
     4081 cgtctttggt gttgttaatg gcctcgtcgc tacttttggc gcaggagcag atgatcgtca
     4141 tgccgatcgc cgccaaaaaa acgaacgaga gaaagatggc ggatgatgta ttcgagaatt
     4201 tcaccatgct cttttcctcc ataaagggcg ttcaagcggt cagttatgag aaagaactgt
     4261 cgcccaagga cgccgcgaga tgcaaggatg accttaaatg catgctcaag gtcgccaagg
     4321 aggccgacgc ggcttattat ctcctgagca agatctcgtc ggacagcatc atggtctatc
     4381 tcatgagtgg tgccggcaag aaactcggca ccgaggatgt cgaatacagc aaagatgccg
     4441 atgaagagga tattgcggcg aagatgctgg cagccactaa gaaactggtt gacaaatata
     4501 ccggttccgg ctcaagcagt tcttcctcgt ccgcgagatc gagtcgcggc agcacctcca
     4561 agacctacac ctattccgag gtcaaggaag agataaagcg gggcatggat ttttatcgca
     4621 agggcgacct gtcgggtgcc gaggaatcgt tctaccgcgc cgccaacgac atgaactgca
     4681 agtgcagcca gaatgacgac gccaagaagc actacgagaa catcgtcaac atcaagaaag
     4741 ggctctcggc cgccgacgat tcggtttcct cgcaggacta cgggaaggcg gagcgtatcc
     4801 tcaagacgat actcgatctc gatacgcagg tccgtgaggc ggggtataaa ctgatgtcct
     4861 tcaaaaagga caatgtggaa cgcgtcatat accgtaaacc caacagcgac gatgtggcgg
     4921 cggtcgattc gatacacaac cagttcaagc ggaagataga ggatgtgcgt aaatggcgcc
     4981 ttgagcaaca ggcctcggta gacaaatggg tgaacgaccg catcaaggcg cgcgaggcgg
     5041 cccttgccga gagagagaag aaacttcagg acttcgtggc catgtccaag gaggccgaga
     5101 aggcgctcaa agaaaaggtg cagggactca agtatcagtg ggagaaggac gattcgtccc
     5161 ttgagcagga gatcgtcaac ctcgaaagcc agatcaccat gatcgaacaa cgcgagaaag
     5221 gcgttgtgaa ggctgcgaac aaagggcgcg agaacgcgca gaagaaggaa tacgaagcgc
     5281 tggacaagaa aaacgacgag atccgcaaaa agatacaggc ggaaaaggag gcctactaca
     5341 acaagcagaa ggagtgggag gacgctgagg gcaaaaaggt gacggcgaga atagccgaca
     5401 tcgag
//
LOCUS       DAOQUE010000161         5353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16898_length_5353_cov_4.587203, whole genome shotgun sequence.
ACCESSION   DAOQUE010000161 DAOQUE010000000
VERSION     DAOQUE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16898_length_5353_cov_4.587203"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PKH10_06170"
     CDS             <1..536
                     /locus_tag="PKH10_06170"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNT27746.1"
                     /translation="RFTCRRCGACCIGDGSVFLLPDDVRAIARHLGIPLQETVDRYTE
                     FLILELRDEQQGYLYLPYLTLRKDEKRACLFLRDKLCSIEPAKPAQCRETPFVEEFFT
                     DAEWRETLRRDCPALRAMPQDRLPRPEDFPGAPLRERAYLDLLREHRYSLEEILGVTL
                     PGPRLVFDTEQDTHAPR"
     gene            559..1011
                     /locus_tag="PKH10_06175"
     CDS             559..1011
                     /locus_tag="PKH10_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27747.1"
                     /translation="MKQDQYRMRPEQAASRIILWGLILSLLASCATMKKPDAEPVNDP
                     CKKLYHDALYGKKEELAGGILLPGGLLLTSSTIYFIPFGATKHDDALLGFGWFALIAG
                     PTAVIAGIVTLIDGNSRVKEWNESCMAQAGDDRYCLQYLGAPPSLPFP"
     gene            1306..>5353
                     /locus_tag="PKH10_06180"
     CDS             1306..>5353
                     /locus_tag="PKH10_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNT27748.1"
                     /translation="MNKKSLFRSRVVVLTRLWGIIFFLLVSNVVSAAEALFPWDYSGI
                     SYFIDPLPFQDVAGNQHAILRGMNGGITLYQNMDAVPLNPFQDYAVGSSAAFASYNNN
                     CGPVATFNVMRYYGITRGPGNVDMSNDSTAASILYAMLDTNSSSCNGLTGPFPGTHPY
                     KIVDFLSATMIGYDVYRKVPLPFSAYSPNREKEMEVFNTIALPLSRGNPVMALITPIG
                     GGLHWVSITGFDTNAYSSLSGSNTRVILGNNDEMAWDEFVSALQFLYDDAWFLFAPIL
                     DACGISPFTFIYAIRPEDDHWDLTISDISIDLPESESDRYTDPINMIPLSYYRDVCSD
                     NFRTSYPNKKHKYLSFTYEKRTALKAWLTCYFNGVEMESGSVFTEGYNFSTPEPLPCE
                     AQKIHANLNANLFTGKNYLRCAWLERDKFTTPYSLVWSGDCNLAPPFLVRDKTFGVRF
                     LLHTSDNDYLPSDIGDEYVAEYRPAMLVKRWLLDSSANCPADGDGDHWFAKYLCTETD
                     TQPCDCDDERLTCRDSDEDGFCDTLAYSNGCSPYDIDWHEPIDNCPGIPNADQADSDL
                     DNVGDVCEDEDSDGVYDITDNCLGVYNPMVPISGEVAFGNLGLDNTANGAFLNPVTRL
                     YTKTVYSPANFFTVPTYYWQPDHDLDGDGDACDLDTAYVTPLEVNSSMHISVSGSNKL
                     YTMNDYLTLKVQATTNTTTNKIVPSTNRYCWVTKQEYNTKPSLWGTPGYCSTYAPPAL
                     GWPLNRLTAFGYSHGSEIEPVRLNKTVWNEISWSSDSGRVLTANRETYHDEENDAYDL
                     SFFPNLIGNLKIPLSYPFQNLSVNLTSPITPLHWNWKTDVVTDKPEYSGNVFSGSEPI
                     MGHPVSPPLANNDFFFAFSSGARDVNSALNYLNDVSCASADNCNNKVNSLYFRINPSA
                     YPNYELKQFARSIRMMSEPMGISYLQFMTGPLGSDWVLDFWKRHHVRLPDFSLDPKLG
                     SYAVRRWEMDSSIVVSLPKYHDERMVILIPGEEGFTYGIRPDDDPNTPATFHIVVNYP
                     SDPADWRPIGFFELSHADYEILAAAQHNGDFYALLKEAGDIRLVKASLANSETAEWQV
                     EMLALAPAELTDPTIHWIGDMLHILGITEDGMGLHRFENGAFVPANSTLLPPTRDFYN
                     AYDSGDALYLAGGKKNNLTTGIPTYLKDIWRYTAAAGWVKLADNLNTELLKTLIRVDG
                     DTLFLANQMIASGNMTQRVIVDLAAPIGSNTSYDMVEVHGLTPEEVPYYCLNEMDTIL
                     KGGTLVDGFCQPFSHPWYKSFSIGTTVYSVAGKSDRLYVGTNSTIRVYDISNPNAMVL
                     KSTFTTNRRVYDLEVAEGDIMYAATSGGI"
BASE COUNT         1275 a         1478 c         1257 g         1343 t
ORIGIN      
        1 accgcttcac ctgccgccgc tgcggcgcct gctgtatcgg cgacggctcg gtattcctgc
       61 tacccgacga tgtccgcgca atcgcgcgcc acctcggcat tcccctgcag gagacggtgg
      121 accgctacac cgagttcctc atcctcgaac tgcgcgatga gcagcagggc tacctctacc
      181 tcccctacct gacgctccgc aaggacgaaa agcgcgcgtg ccttttcctg cgcgataaac
      241 tctgctccat agaaccggcg aagccggccc agtgccgcga gacccccttc gtcgaggagt
      301 tcttcaccga cgccgaatgg cgcgaaacgc tccggcgcga ctgcccggcg ctccgcgcga
      361 tgccgcagga tcgccttccg cgtcccgagg acttccccgg cgcaccgctc cgggaacggg
      421 cctacctcga cctcctgcgc gaacaccgct attcgctcga agagatactc ggcgtcaccc
      481 tccccggccc gcggctcgtc ttcgacaccg agcaagacac gcacgcccct cgataagatg
      541 acgggttggt atgaccgcat gaaacaggac cagtatcgta tgcgccccga acaggcggct
      601 tcccgcataa tcctttgggg gctgatcctc tctcttctcg cctcatgcgc taccatgaaa
      661 aaaccggatg ccgaaccggt caacgatccc tgcaaaaaac tctaccatga tgcgctttat
      721 ggcaaaaaag aagaactagc cggcggcatc ctgctccccg gtggattgtt gctcaccagt
      781 tcgactattt atttcattcc gtttggggcg accaaacatg acgatgcctt gctcggcttt
      841 ggctggttcg ccctcatagc gggacctact gcggttatcg caggcatcgt gacgcttata
      901 gacggcaatt cccgcgtcaa ggagtggaac gaatcctgta tggctcaagc gggcgatgat
      961 cgttattgcc ttcaatatct tggcgctccc ccctctttac ctttccccta gatctttttt
     1021 ctcataacga ttttatctga cctgtttccg ccctaggccg attttcgtgc aaaacttcgg
     1081 tagatctctc gtcgaaacaa aaaaaagacc caacaaaaac aatacggtaa gagaaaaaac
     1141 aggcaggggt gcatcttttc cccgatacga tgtctcttat gagcagattt aggagaagaa
     1201 agttttgttg acaagaaata ccccgattta tttagaaagg cataatagaa acaagaagtt
     1261 gcgctcagag aaacaaatct ttaatgtcaa gttggggaga atgttatgaa caagaaaagt
     1321 ctatttaggt ctcgcgtcgt tgttttgact cgattgtggg gaatcatttt ttttcttctt
     1381 gtgtctaatg tcgtgtcggc agcagaagca ttgtttcctt gggattattc cggtattagt
     1441 tatttcatcg atccccttcc atttcaagat gttgcaggga accagcacgc tattctgcgt
     1501 ggaatgaacg gcggcatcac tctttatcag aatatggatg ctgttcctct caaccccttt
     1561 caagattatg ccgttggctc tagtgcggct tttgcttctt acaataacaa ctgcggtcct
     1621 gttgccacat tcaatgtgat gcgttattat ggcatcactc gaggaccggg taatgttgac
     1681 atgtcgaatg acagcaccgc cgcttcgatt ctttatgcca tgctcgacac gaattctagc
     1741 agttgcaacg gtctcacggg accatttccg ggaacacatc cttacaaaat agtggatttt
     1801 ctttctgcga ccatgatagg ctatgatgtt tatcgaaaag tccctttacc tttcagtgct
     1861 tactcgccga atcgtgaaaa agaaatggag gtttttaaca ccatagctct tccactgagt
     1921 aggggaaatc ctgttatggc cttgattact cccattggcg gcgggcttca ctgggtatca
     1981 ataaccggct ttgataccaa cgcttacagc agcctgtctg ggtcaaacac gcgcgttatt
     2041 ttgggtaaca atgatgaaat ggcgtgggac gaatttgttt ccgctctaca gtttctctat
     2101 gacgatgcct ggtttctgtt cgcccctata ttggatgcct gtggcatatc gccgttcact
     2161 tttatctatg ccattcgccc cgaagacgat cactgggatc ttactatctc agatatatcg
     2221 atagaccttc ccgaaagcga aagcgaccgt tatactgacc caataaatat gataccattg
     2281 agttattatc gagatgtttg ctccgataat tttcgcacaa gctatcctaa caaaaaacac
     2341 aaataccttt cttttactta tgaaaaaaga accgctctca aagcttggtt gacctgttat
     2401 tttaacggcg tcgagatgga atccggaagc gttttcacgg aggggtataa tttttcaacg
     2461 cccgaaccac ttccctgtga ggcgcaaaaa attcatgcca accttaatgc caaccttttc
     2521 acggggaaaa attatttgcg gtgcgcgtgg ctagaaagag ataagtttac cactccttac
     2581 tcgcttgttt ggagtggtga ttgtaatctt gctccgcctt ttttagtgcg agataaaact
     2641 ttcggcgttc gtttcctcct tcatacttcc gataacgatt accttccttc agacatcggc
     2701 gatgagtatg ttgccgagta caggcccgcg atgttagtaa aaaggtggct tctagactcc
     2761 tctgcgaatt gtcccgccga cggagacggg gatcactggt tcgcaaaata cctttgcacc
     2821 gaaacggata ctcaaccatg cgattgtgat gacgagcgat tgacctgtcg tgattcggat
     2881 gaagacggct tctgcgacac gcttgcctat tcgaatggat gcagtcccta cgacatagat
     2941 tggcatgagc cgatcgacaa ttgtcccggc attcccaatg ccgaccaagc cgactccgac
     3001 ctcgataatg tcggcgatgt ttgcgaagac gaagattcgg atggcgtgta tgacatcacc
     3061 gataattgtc tcggcgtgta taatccgatg gtcccgataa gcggcgaggt cgcgtttggc
     3121 aatctcggtc tcgataatac cgcgaacggc gcttttctta atcccgtcac gcggctctac
     3181 accaaaacag tttatagtcc ggccaacttc tttacggtgc cgacctatta ttggcaaccg
     3241 gatcacgatc tcgacggcga cggtgatgcc tgcgatctcg ataccgctta tgtcacacca
     3301 ctggaagtaa attcatcgat gcacataagc gtttcgggaa gcaataaact ttacaccatg
     3361 aacgactacc tcacactcaa ggtgcaggca acgacgaaca ccacgacaaa caagatcgtt
     3421 cccagcacta accgatattg ttgggtcaca aaacaagagt ataatacgaa gccttctctt
     3481 tggggaactc ccgggtactg ctcgacttac gctcctccgg cgctgggatg gccgcttaat
     3541 cggttaaccg ctttcggtta tagtcatgga agtgagatcg aacctgtgcg actcaacaag
     3601 acggtgtgga acgaaatttc atggtccagc gattccggtc gggttttgac cgccaaccgc
     3661 gaaacatatc atgacgagga aaacgacgct tatgacctct cctttttccc caatctcatc
     3721 ggaaatctca aaataccact ctcttaccct ttccaaaatc ttagtgtcaa cctcacttca
     3781 ccgataactc cgctccattg gaactggaaa accgatgttg tgaccgacaa gcctgaatat
     3841 tccgggaatg ttttttccgg atcagaaccc atcatggggc atcctgtttc tccccctctc
     3901 gcaaacaacg atttcttctt cgctttttcg agcggtgcgc gcgatgtcaa ctctgccctt
     3961 aattatctca acgatgtttc gtgcgccagc gcggacaatt gtaacaataa ggtaaattct
     4021 ctttatttcc gcatcaatcc ttccgcatat ccgaactatg aattaaaaca gttcgcccgc
     4081 agtatacgca tgatgagcga gccaatgggg atctcttacc tgcaatttat gaccggccct
     4141 ctgggatccg attgggtact cgatttctgg aaacgccacc atgtccgcct tcccgatttc
     4201 tcgctcgatc ccaaactggg gagttatgca gtgcgtcgct gggaaatgga cagttccata
     4261 gttgtctcgc ttccgaagta tcacgacgag cgtatggtca tcctgatccc cggcgaagaa
     4321 gggttcactt acggcataag gcccgacgat gatccgaaca ctcccgcgac ctttcatatc
     4381 gtcgtgaact atccgagtga ccccgctgat tggcgcccga tcggtttctt cgaactatcc
     4441 catgccgatt acgaaatact tgcggcggcg caacataatg gcgattttta tgcgctcttg
     4501 aaagaagcgg gcgatattcg gttggtaaaa gcatctctcg caaatagtga aaccgccgag
     4561 tggcaggtcg agatgttggc tcttgccccc gccgagctga ccgatccgac gatccattgg
     4621 ataggcgata tgctgcatat cctcggcatc acagaggacg gtatggggct ccaccgtttt
     4681 gaaaacggcg cttttgttcc cgcaaattca acgcttctgc ccccgactcg cgacttttat
     4741 aacgcctatg attcagggga cgccctgtat ctcgccgggg ggaaaaagaa caacttaacg
     4801 accggcatcc caacttatct taaagacatc tggcgctaca ccgccgccgc cggctgggtg
     4861 aaactcgccg ataatctcaa caccgaactc ctcaaaacac ttattcgcgt agatggggac
     4921 acgctttttc ttgctaatca aatgatcgcc agcggcaaca tgacccagcg ggtgatcgtc
     4981 gatcttgcag cgccgatagg gagcaacacc tcctacgata tggtggaagt gcatggtctt
     5041 accccagaag aagtgccata ctattgcctg aacgagatgg atacgattct caagggcggc
     5101 acgctcgttg acggtttctg tcagcccttc tcccatccgt ggtacaagtc cttctccatc
     5161 ggcacgaccg tctacagcgt ggcgggtaag agcgaccgtc tctatgtcgg gacgaacagc
     5221 acgatccgcg tctacgatat cagcaacccg aacgcgatgg tcctcaagag caccttcacc
     5281 accaaccggc gggtctatga cctcgaagtg gccgagggcg acatcatgta cgcggcgacc
     5341 agcggcggca tct
//
LOCUS       DAOQUE010000162         5352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16901_length_5352_cov_5.656409, whole genome shotgun sequence.
ACCESSION   DAOQUE010000162 DAOQUE010000000
VERSION     DAOQUE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16901_length_5352_cov_5.656409"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5352
                     /locus_tag="PKH10_06185"
     CDS             <1..>5352
                     /locus_tag="PKH10_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HNT27749.1"
                     /translation="FIAGVSGYWRFSVYSTFDAVIDLYEGACTSYIDCEDYSGANATD
                     WMEYPLTAGQVYTVVVDKYSTSDSGTYTLDIMPPTDRGKKAFSWIDDTDTTYTNWDTN
                     EPSGGTEGCVEMKIATTTNAKWNDLACNSSSVKLAYTCETTGNILALQNKEAITELDL
                     SNRGLTSLEGISAFTGLQALNASYNNLSNVSYLAENISVTTLNVSHNPIVDVDAFLQL
                     PNLQQVTISHSSDEDDIRIISINPGENLEDFIYRIVITAGIPLPLDTYTELSSLLVHY
                     RFGVSYGGKYTRNAASGYEDLYWGFSPTDFKSVSYSDWPFGVAAEFDGNDQMIVFNHP
                     DDYDTDHMLTMDAFSTGSDVTEDFSISLWVEPDNYGWERIFDHCMNMKCDETNLSIAY
                     NKDPDYPSNPPLLAFYPSIDNQYEMSLTNLSNLYSGWIHYVYTYDASSTIAAIYINGD
                     LIISGGSNIDNTANEVKYFTIGKNSRSSFDEFRIHASVLTQKEVQDLYYFETCPDGMD
                     HIGPDGACQSTCGDGELAPDEECDDGANKNNTDGCYDNCKLGSVPRGFLDVTDKTNIE
                     GWSCDPEDWNATITITLKFYDNDGVLRTTASSSGTPSEFSVVDRPDLITNGDVCNGTI
                     SHGFKYNATTIIEQMETAGYPKPYTVRAFASNIFTFPSGLESVELPRSDPLADYVVVF
                     CGNTIIEDATEQCDDGNAITEVCPYGVETCTVCDATCQNIAGDTSYCGDGVKDADNGE
                     ICDDGASNGTYGHCNSLCSGLGERCGDGIPQYEDGEFCDDGDGNNQNSCKNDCTLNIC
                     GDNVEYTGVEQCDDGRNGNNCDGCLDNCTQHINTCGDGYFCGTEECDDGNTDNTDGCV
                     EDCLLATCGDGFIRAGVEQCDDGILNGVYGYCNDTCSGIGEHCGDGIVNGTEICDDGT
                     NNGEYDYCNSDCSGDGPYCGDSIVNGEEECDDQNAIDSDDCTSECEFNVCGDGILKTS
                     SSDPLSIEECDDGNTSNADTCADNCQQFRISVSRPDDYFPTYYVGQRQKLRVEVTPPR
                     EGVPVYMTRQRYHNGNTVTQIWDTPRFTDANGRILETIDPIPSSMVGAYSGEEFRAGA
                     ADAANYAGPLSYTISASKTENCGDLTVSHTQWIEPDGVNPSTGEFSSSWDETAQTFMP
                     QTSDCVWGCADGYALEKSAGAYLTPADLLDLRNEVDRFWRVKVIMKHLAGAADMAGQS
                     WEDLSNRYLYLYKMCSSVELKHILKEHDPMQANTYPDSYYEKSLKLLLSDLNGIAAAG
                     FIRLIHDGDRYLAYDPLSNMGYPEGTEITLYHLDEPLFAERVAEMNSLLDKHLYSILF
                     NNEELFYTNRLVLEAIFFDWDTNHNVYYPVIPFGHPKGSEVCLDQKMAECRKQTASLF
                     KLTHIEGTTTYVPEYYYYYYSDNKVTDVVAEYEDGEWIVPACEWVCKPGTVKASTGDL
                     SQCVPESDIDFEDAKLKSCVLNAITQMGNSEQKSYGEWVLSVCRTCEESEDGCDPTTQ
                     AVCAIVEGAQTAQQVAALLGVNLILQPSSPGKIYLADVKKIKKLDCHYDWNAPEKTIP
                     AMGTIEPLSNLTSEYIRDIEGLQHFTALEDLILSGQSVIDLTPLRDIRRIDYVHVPFG
                     AKICGEETFVVLRNFDLDETDENALRTQNMVSYRSHPENGGLLEDGIGFTFGFHSITN
                     DSDFKTIFLSDPYQGKEEDLNFKNAKVYGCDSTSTQGYVTEIYHATEDPENPGNVIGE
                     RKQSWVVCSNSDQYFPAFTFRKAVSYDSFEDYAETECPQSTTDGIWHYVKVELGLSHT
                     "
BASE COUNT         1414 a         1339 c         1347 g         1252 t
ORIGIN      
        1 ttcatcgccg gggtaagcgg ctactggcgt ttcagcgtct attcgacctt cgacgcggtg
       61 atcgacctct acgagggcgc ctgcacctcc tacatcgact gcgaagacta ctccggcgcg
      121 aacgcgaccg actggatgga atatcccctt accgcagggc aggtatatac cgtcgtggtt
      181 gacaagtaca gcacgagcga ttcgggaacc tacacgctcg acatcatgcc gccgaccgat
      241 cgcggcaaaa aggccttctc atggatagac gataccgaca cgacctacac caactgggac
      301 acgaatgaac cgagcggcgg caccgaaggg tgcgtcgaga tgaagatagc gacgacgacc
      361 aacgccaagt ggaacgacct cgcctgcaac agttcatcgg tgaaactcgc ctacacctgc
      421 gagacgacgg gtaacatcct cgcccttcag aacaaggagg cgatcaccga actcgatctc
      481 agcaaccggg gactcaccag tttggaaggg ataagtgcct tcaccggttt gcaagctctg
      541 aatgcgtcat acaataatct ttccaatgtc tcctatctgg ccgaaaacat ctctgtcacc
      601 acgctgaatg tttcccataa cccgatcgtc gatgtggatg cgttccttca attgcccaat
      661 ctgcaacagg tgacgatcag ccatagttcc gatgaggacg atatcagaat catttcgata
      721 aatccgggcg agaatctgga ggactttatc tatcggatag ttattacggc gggcatccct
      781 cttccactcg atacttatac agaactttct tctcttttgg ttcattatcg cttcggcgtg
      841 tcttacggtg gcaaatatac cagaaatgcg gcgagcggat atgaggattt atactgggga
      901 ttttctccca cggatttcaa atcggtttct tattccgact ggcctttcgg tgtcgcggct
      961 gaattcgacg gaaacgatca aatgatagtg ttcaatcatc cggacgacta cgatactgac
     1021 cacatgctaa caatggatgc tttcagtaca gggtcagatg taacggagga tttctccatc
     1081 agtttatggg ttgaacccga taattatggg tgggaaagaa tttttgacca ctgtatgaac
     1141 atgaaatgcg atgagacaaa tttatcgata gcctataaca aagaccccga ttatccttcc
     1201 aatcctcctt tgttggcatt ttacccgtcg atagacaatc aatatgagat gtctttgacg
     1261 aatctttcga atctgtattc gggatggatt cattatgtgt atacctacga tgcctcttct
     1321 accatagctg cgatatatat aaacggggat ctcattatca gcggcggaag taatattgat
     1381 aataccgcaa acgaagtcaa atacttcact ataggaaaaa acagtcgttc atcttttgat
     1441 gaattccgga tacatgcttc ggtcttgacg caaaaggaag tacaagacct ctattacttt
     1501 gagacatgtc cagatggtat ggatcacatc ggacccgacg gtgcctgcca gagtacctgc
     1561 ggtgatggcg aactggcccc tgacgaagaa tgcgatgacg gcgcgaacaa aaacaatact
     1621 gacggttgct acgacaactg caaactcggt agtgtgccga gaggatttct tgatgttacg
     1681 gataaaacaa acatcgaagg atggtcgtgc gatccggaag attggaacgc aacaataacg
     1741 ataacattga aattctacga taatgacggc gttcttcgta ctacggcttc ttcttcagga
     1801 acaccgtcgg aattttcggt ggtagataga cccgacctga tcactaacgg tgatgtctgt
     1861 aatgggacca tatctcatgg attcaaatac aatgccacga ctatcattga gcagatggaa
     1921 actgccggtt atcctaagcc atacacagtg cgtgcttttg ccagcaacat ttttaccttc
     1981 ccctccgggt tggaatctgt cgaacttccc cgtagcgatc ctctcgccga ctatgtagtg
     2041 gtgttctgcg ggaatacaat aatcgaagac gctacagaac aatgtgacga cggcaatgcc
     2101 ataaccgaag tttgtccata cggtgtcgaa acctgcactg tctgcgatgc gacatgtcaa
     2161 aacatcgcgg gggacaccag ttactgtggt gacggtgtta aagacgccga taatggagag
     2221 atttgtgacg atggcgccag caatgggacc tatggacatt gcaattcact ctgttccggt
     2281 ttgggagaac ggtgcggtga cggcatcccg cagtatgaag acggagaatt ttgcgatgac
     2341 ggagacggga acaaccaaaa ctcctgcaag aacgactgca cattgaatat ttgcggcgac
     2401 aatgtcgaat acaccggcgt tgaacaatgt gacgacggac ggaacgggaa caattgcgac
     2461 ggctgcctag acaactgtac gcagcatatc aacacctgtg gcgacgggta tttttgtggc
     2521 acggaagaat gtgacgacgg caataccgac aacaccgacg gttgtgttga ggactgtctg
     2581 ttagccactt gcggggacgg ctttattcgg gcaggagtcg aacaatgtga cgacggcata
     2641 ttgaacggtg tttatggtta ctgcaacgac acttgttccg gcatcgggga gcactgcggc
     2701 gacggtatcg taaacgggac tgagatttgc gatgatggca ccaataacgg cgaatacgat
     2761 tactgtaaca gtgattgctc cggtgatggt ccttactgtg gtgatagtat agtcaacggc
     2821 gaagaagaat gcgacgatca aaatgccatt gattccgatg attgtacaag cgaatgcgag
     2881 ttcaatgtct gcggtgacgg catcctgaaa acttcttctt cagatcctct ttccatcgag
     2941 gaatgcgatg acggcaacac cagtaatgcc gatacctgcg ccgacaactg tcagcaattc
     3001 cgcatatcgg tatctcgtcc cgacgattat ttcccgacct actatgtcgg tcagcgacag
     3061 aaattgaggg tggaagttac acctccgcga gaaggtgttc ccgtctacat gacccgccag
     3121 cgttaccaca acggcaatac cgttacgcag atctgggaca cgccaagatt taccgacgcc
     3181 aacggtcgga tcctcgaaac catagacccg ataccgtcaa gcatggtcgg cgcttattcc
     3241 ggtgaggagt tccgtgccgg tgccgccgat gccgccaact acgccggccc cttgagttat
     3301 acgatatccg cttccaagac ggaaaactgc ggcgatttga ccgtctctca tacgcagtgg
     3361 atcgagccgg acggcgtaaa tccatccacg ggagagtttt cttcgtcttg ggatgagacc
     3421 gcgcaaacct tcatgccgca gacttcagat tgtgtatggg gttgtgccga cgggtatgct
     3481 cttgaaaaga gcgccggggc atatctgacg ccggccgacc ttctcgacct gagaaacgaa
     3541 gttgatcgtt tctggcgggt caaagtgatc atgaagcatc ttgcgggagc cgccgatatg
     3601 gcgggacaat cttgggagga tctgagcaac aggtatcttt atctctacaa aatgtgttct
     3661 tcggtcgaac tgaagcacat tctcaaggaa catgatccga tgcaggcgaa cacttatccc
     3721 gattcatatt acgagaaatc tttgaaacta ttgctttccg atctcaacgg aatcgcggcc
     3781 gccggattca tcaggctgat acacgacgga gatcgctacc ttgcctacga tccattatcc
     3841 aacatgggct atcccgaagg caccgagatc acgctctacc accttgatga accgctcttt
     3901 gccgagcgtg tcgccgaaat gaattccctg ctggacaagc acctttattc catccttttc
     3961 aacaacgaag agcttttcta taccaaccgc ttagtgctgg aggctatatt cttcgattgg
     4021 gatacgaatc ataatgtata ctaccccgtg atcccattcg gacatcccaa aggcagcgag
     4081 gtatgcctcg accaaaagat ggcggaatgt cggaaacaaa cggcttctct tttcaaactc
     4141 acccacatag aaggcaccac cacctatgtg ccggagtact actattatta ctattccgac
     4201 aacaaggtca ccgatgtcgt cgccgagtac gaagacggtg aatggatcgt tcctgcctgc
     4261 gaatgggttt gtaagcccgg tacggtaaag gcgagcacgg gagatttatc gcagtgtgtg
     4321 cctgaatccg atatcgattt cgaagatgcc aaactcaaat cctgcgtact gaacgccatc
     4381 acccaaatgg gaaattccga gcagaaaagt tacggcgaat gggtgttgag tgtttgccgt
     4441 acctgtgaag aaagtgaaga tggttgcgat ccgaccacac aggccgtttg cgcgatagta
     4501 gaaggggcac agacggcgca acaggtggcg gcattattgg gggtcaatct gattcttcaa
     4561 ccatcctctc cgggaaaaat ctatctagcc gatgtcaaaa agatcaaaaa acttgactgt
     4621 cactacgatt ggaacgctcc cgaaaagacg atcccggcaa tgggaaccat cgagcctctc
     4681 tccaacctga ccagtgaata catccgagac atcgaagggc ttcagcattt cacggcgctc
     4741 gaagacctca tcctttcggg gcagagtgtc atcgacctga cgccgcttcg cgacatccgg
     4801 cggatcgatt atgtacatgt gccgttcgga gcaaagatct gcggggagga gacttttgtc
     4861 gtcctgcgca acttcgatct cgacgagaca gatgaaaatg cgttgcgcac acagaacatg
     4921 gtttcctaca ggagccatcc ggaaaatggc ggattgctcg aagacggtat cggtttcact
     4981 ttcgggttcc acagcatcac caacgactcc gatttcaaga ccattttctt gagcgatccc
     5041 tatcagggga aagaagagga tctgaatttc aaaaacgcaa aggtctatgg gtgcgacagc
     5101 acgagcaccc aaggatatgt cacggagatc taccatgcca cggaagaccc cgaaaatccg
     5161 ggcaatgtga tcggcgagag aaaacaatcg tgggtagtct gttccaatag cgaccagtat
     5221 tttcccgcct tcactttccg taaggccgtg tcttacgatt cgtttgagga ttatgccgaa
     5281 acggaatgtc ctcaatccac aaccgatggt atctggcatt atgtgaaagt ggaactgggt
     5341 ctttcgcata cc
//
LOCUS       DAOQUE010000163         5349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16916_length_5349_cov_4.906687, whole genome shotgun sequence.
ACCESSION   DAOQUE010000163 DAOQUE010000000
VERSION     DAOQUE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16916_length_5349_cov_4.906687"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="PKH10_06190"
     CDS             complement(<1..1651)
                     /locus_tag="PKH10_06190"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNT27750.1"
                     /translation="MGILGQSNFRARRWFGKITKRVKDIPDLLQVQKSSFVQFLQKDA
                     APSERTVTGLESAFRSVFPITSFNNAIELTFGGYSLGEPLYDVEECKLRGTTYEIPLK
                     IKVALASYEVDQNGVRKGLNDIKEQDIHFGAIPAMTDWCTFVINGTERVIVNQLHRSP
                     GTFFDYDKMRSAAGNDVFVSRLVPYHGSWLDFEFDSKNLLYVKIDRKKKFPATVFLKA
                     WGYSKEDLVSSYYFPERFVINKGEIFQVLDHEKDAEQLKEQKVPVDLYDKSGALIVQE
                     GKNFSSAVLLKLKKLGIKDIAIDPQHLMGKRLFKPVADKNGTRIADVNDEITPTLLDE
                     ILKRGVQRIETIYIENMKYSSHLSDTLKIDKVSTREEALTEIYKIMVPSNPVNIDVAE
                     AYFNNLFFNSENYDLSAVGRLRMNHKFGEQVPLTNRLLRREDVLNTVRYLLNLTDGRG
                     IVDDIDHLGNRRVRLVGELLEDVFKNGLLRMAHSIKEKLAGAAIESLTPYEVIGYKQV
                     TAVLKEFFAGGQLSQFMDQTNILSEMTHKRRLSALGPGGLAR"
     gene            complement(1787..2158)
                     /gene="rplL"
                     /locus_tag="PKH10_06195"
     CDS             complement(1787..2158)
                     /gene="rplL"
                     /locus_tag="PKH10_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNT27751.1"
                     /translation="MATKAEVKEFIKGMTVLELAELIKELETELGVSAAAPVAVAAAA
                     PAAAAAEAQTEFTVVLKSAGAKKLEVIKEIRALIPTLGLKEAKDLVDGAPKTVKEGVT
                     KDEANKIKEKLVAVGAEIEIK"
     gene            complement(2195..2731)
                     /gene="rplJ"
                     /locus_tag="PKH10_06200"
     CDS             complement(2195..2731)
                     /gene="rplJ"
                     /locus_tag="PKH10_06200"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNT27752.1"
                     /translation="MEKARKEQLVAELKKLLSGASSCIAVDFGNVPMTTFTPLRKDCA
                     KNGVKFVVVKNTLARIAVKETAYAGMTEFFKGMTSLVVTTMDDQTVGAKLLKKYAEKD
                     KDKGFVVKGGMIDGRVLSAQEVKILSEMPGKTELQAKLLGTMQAVPQKFVQLLAAVPQ
                     SFLRVLVAHKEKMETAGN"
     gene            complement(2852..3559)
                     /gene="rplA"
                     /locus_tag="PKH10_06205"
     CDS             complement(2852..3559)
                     /gene="rplA"
                     /locus_tag="PKH10_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNT27753.1"
                     /translation="MTTRQGKRIADVAKTYDHAKKYHFSEAFDLMQKAASKKFDETVD
                     LAINLGIDPKKSDQMVRGSCVYPHGLGKKVVVLAIVPPDKVEEATKAGADFVGGDDMI
                     EKIQSESWVDYDKLLTTPAMMGKVGKLGKVLGRRGLMPNPKVGTVTNDIAEAIKMAKA
                     GKVEFKVNKAGVLCVPIGKVSFGSQKLQENAEELFRSLVKLKPSTAKGAFIKKISLSS
                     TMGPGVKIDENDLLEKV"
     gene            complement(3572..3994)
                     /gene="rplK"
                     /locus_tag="PKH10_06210"
     CDS             complement(3572..3994)
                     /gene="rplK"
                     /locus_tag="PKH10_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNT27754.1"
                     /translation="MAKKIIANIKLQIPAGQANPAPPVGPALGQHGVNIMEFCKQYNA
                     KTQAQKGFIIPVIITVYADRSFSFVTKTPPTSQLLLKAADKEKGSGEPNKKKIGAVSR
                     KAVEDIAKTKLEDLNTTDLESAKRSVEGTAKSMGITIE"
     gene            complement(4010..4570)
                     /gene="nusG"
                     /locus_tag="PKH10_06215"
     CDS             complement(4010..4570)
                     /gene="nusG"
                     /locus_tag="PKH10_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNT27755.1"
                     /translation="MKWYAIHTQSGQENKLRNAILKIVEDRKMQDHFGEIIVPEVEIE
                     EKKGDKKRKVRKNLYPGYVFIQMHYSDEAWLLIKSVAFIRKPAFVGEKKRFDKNAGIK
                     PTPITAVEIERIRKMMQDGEIKKVHEISFEKGETILIKDGAFAGFKAVIDDIKEGKEK
                     LDVLVNIFGRSTPVELSFSQVEKVED"
     gene            complement(4594..4977)
                     /gene="secE"
                     /locus_tag="PKH10_06220"
     CDS             complement(4594..4977)
                     /gene="secE"
                     /locus_tag="PKH10_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNT27756.1"
                     /translation="MKNSKIILVSYAVFFALMTYFAVSLSDAVLSIRSIDLADYLLAG
                     FLPLKNVVGIVIAVATTWFLWAYRQGTYRSQLDEVIEELKKVTTPTKDETRVTTISVF
                     VFVGIMIVVFFVLDLIWANVMLYIY"
     gene            complement(5016..5091)
                     /locus_tag="PKH10_06225"
     tRNA            complement(5016..5091)
                     /locus_tag="PKH10_06225"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5056..5058),aa:Trp,seq:cca)
     gene            complement(5104..5280)
                     /gene="rpmG"
                     /locus_tag="PKH10_06230"
     CDS             complement(5104..5280)
                     /gene="rpmG"
                     /locus_tag="PKH10_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNT27757.1"
                     /translation="MASKKKAGNRIKLTLVCSECKRKNYTTTKNKKNTADKVVLQKYC
                     PHDRKHTEHKEGKI"
BASE COUNT         1055 a         1558 c         1363 g         1373 t
ORIGIN      
        1 cgcgcgctag accaccggga ccgagtgccg acagacgccg cttatgggtc atttcggaca
       61 ggatgttggt ctgatccatg aactgggaaa gttggccgcc ggcgaagaac tctttgagca
      121 ccgccgtcac ctgtttgtat ccgatgacct cgtagggcgt gagcgactct atcgccgcac
      181 cggccagttt ctcttttatg ctgtgagcca ttcgcagaag accgttcttg aacacatcct
      241 cgagaagttc gccgaccaga cgcacacgac ggttcccgag gtggtcgatg tcgtccacga
      301 tgcctcgacc atcggtaagg ttcaacaggt aacgcaccgt gttgagcacg tcttcgcggc
      361 gcaggagtcg attggtcagc gggacctgtt caccgaattt atggttcatt cgcagacgac
      421 cgaccgcact gagatcgtag ttctccgaat taaagaacaa attattgaaa taggcctcag
      481 cgacatcaat gttcaccgga ttggagggaa ccatgatctt atatatctcg gtcaaagcct
      541 cctcacgggt cgacaccttg tctatcttca gcgtatccga aagatgcgag gagtacttca
      601 tgttctcgat gtagatggtc tctatgcgct gaacgccccg cttgagtatc tcatcgagaa
      661 gcgttggggt gatctcgtcg ttgacatcgg cgatccgcgt gccgtttttg tccgcgaccg
      721 gcttgaacag tcgtttcccc ataaggtgct ggggatcgat ggcgatatcc ttgatgccca
      781 gtttcttgag tttcagcagg accgcgctgg agaagttctt gccttcctgc acgatgaggg
      841 cacccgactt gtcgtaaaga tcgaccggga ccttttgctc cttgagttgt tccgcgtcct
      901 tctcgtggtc caacacctga aagatctcgc ctttgttgat gacgaatctt tccgggaaat
      961 agtaactcga aacgagatcc tcttttgagt agccccatgc cttaaggaaa acggtggccg
     1021 ggaatttctt tttgcggtct atcttgacat acagcaggtt cttggagtcg aactcgaaat
     1081 cgagccatga accgtggtac gggaccaacc gggaaacgaa cacatcgttg ccggcggccg
     1141 aacgcatctt gtcgtagtcg aaaaaggtcc ccggagagcg gtgtaactga ttgacgatga
     1201 cccgctccgt accgttgatg acgaaagtgc accagtcggt catggcggga atggccccga
     1261 aatggatatc ctgctccttg atgtcgttca gccctttacg gacgccgttc tggtccacct
     1321 cgtaggaggc gagcgccacc tttatcttga gaggtatctc ataggtggtg ccgcgcagtt
     1381 tgcattcctc aacatcatac agcggttcgc cgagcgaata tccgccgaag gtgagttcga
     1441 tggcgttatt gaaactggtg atcgggaaga ccgagcgaaa agccgactcc agaccggtga
     1501 ccgtccgttc ggacggcgcc gcgtcctttt gcaggaactg cacgaaggaa ctcttttgga
     1561 cctgaagaag gtcggggatg tcctttaccc gtttcgttat cttcccgaac caccgacgag
     1621 ccctgaaatt agactgaccg aggataccca ttgcaacctc tcttgcgtgt tagttgccga
     1681 tatgcgtccc tgtacacaaa gtcaaagaac aaaaatacgg taagacagca tacaccgctg
     1741 tcttaccata caaaacatat gaactgtccc gtaacaccgg gacgagttac tttatctcga
     1801 tctcagcgcc gaccgcgacc agtttctctt ttatcttgtt cgcctcgtcc ttggtcacgc
     1861 cttccttcac cgtcttgggc gcgccatcga ccagatcctt cgcttccttg aggcccagcg
     1921 tcgggatcag cgcgcggatc tctttgatga cctcgagctt cttggcgccc gccgacttca
     1981 gaacgaccgt gaattcggtc tgagcctcag ccgccgccgc cgccggggcc gccgccgcta
     2041 ccgcgaccgg cgccgccgcg ctgacgccca gttcggtctc aagttccttg atgagctcgg
     2101 ccagttcgag gaccgtcatt cccttgatga attctttcac ttccgctttg gtagccatga
     2161 tctttctctc ctttgaaaaa agttgtaacg ctcgttaatt gcctgctgtt tccatctttt
     2221 ctttgtgagc cacgagtacc cgcaggaacg actgggggac cgccgcaaga agttgcacga
     2281 acttctgcgg gaccgcctgc atcgtaccta agagtttcgc ctgaagttcc gtcttcccgg
     2341 gcatttcgga aaggatcttg acctcctgtg cggaaagaac ccttccatcg atcatgccgc
     2401 ctttgacaac gaaccccttg tccttgtcct tctctgcgta cttcttcagg agtttcgcgc
     2461 cgaccgtctg gtcgtccatc gtggtgacga cgagcgaggt catccccttg aagaactcgg
     2521 tcatgccggc ataagccgtc tctttgaccg cgatcctcgc gagggtgttc ttgacgacga
     2581 cgaatttgac gccgttcttg gcgcaatcct tacgaagcgg ggtgaaggtg gtcatgggca
     2641 cattgccgaa atcgaccgcg atgcatgacg aagcgccgga aagaagcttt ttcagttccg
     2701 cgaccaactg ctcctttctc gctttttcca tctaacctct cctcttgttc tgtcgctggc
     2761 ccgcgaagac agaaacaact tgtctccggc aggagattaa gccggcagtg ccggcacccg
     2821 ccttctcaga ccaggatcgt tccaactctt attacacctt ttccaacaga tcgttctcgt
     2881 cgatcttcac gccgggcccc atggtgctcg acagcgagat cttcttgata aaagctcctt
     2941 tggcggtcga cggtttcaac ttgaccagac ttctgaacag ctcttcggcg ttctcctgaa
     3001 gtttctggga gccgaaactc accttgccga tcggcacgca gagcacaccg gccttgttga
     3061 ccttgaactc gaccttgccc gccttcgcca ttttgatcgc ctcggcgatg tcgttggtca
     3121 ccgtcccgac cttaggattg ggcatcagac cacgacgacc gagaaccttg cccagtttac
     3181 cgaccttgcc catcatggcg ggggtcgtca gcagtttatc gtaatcaacc cagctctcgc
     3241 tctgtatctt ttctatcata tcgtcgccgc cgacgaaatc ggcgccggcc ttcgtcgcct
     3301 cctctacctt atcgggcggt acgatggcaa gaacgaccac tttcttaccg agcccatgcg
     3361 ggtagacaca ggacccgcgg accatctggt cagacttttt ggggtctatg cccaggttga
     3421 tggcaaggtc caccgtttcg tcgaattttt tcgaagcggc tttctgcatg agatcgaaag
     3481 cctcgctgaa gtgatatttc ttcgcgtgat cgtaggtttt cgccacatca gcgatccttt
     3541 tgccttgtcg cgtcgtcatg atgtcgtctc cctattcgat ggtgatgccc atgcttttcg
     3601 cggtcccttc caccgaccgc ttggccgact cgaggtcggt cgtattaaga tcctccagct
     3661 tggtcttggc gatatcttcg accgccttgc ggctcactgc gccgatcttc tttttattcg
     3721 gctctcccga tcccttctcc ttgtcggccg ccttgagtag aagttgcgag gtcggcgggg
     3781 tcttggtcac gaacgagaag gaacggtcgg catacaccgt gatgatgacg gggatgatga
     3841 atcctttctg agcctgcgtt tttgcgttat actgcttgca gaactccatg atgttgacac
     3901 cgtgctgacc gagcgcgggg ccgaccgggg gagcggggtt cgcctgtccg gcgggtatct
     3961 gcagtttgat gttcgcgatg atcttcttcg ccatgggatt ctcctttggt taatcctcga
     4021 ctttttccac ctgggaaaaa ctcagttcga caggcgtcga ccgcccgaat atgttcacga
     4081 gcacatccag tttctctttt ccttctttga tgtcatcgat gaccgccttg aagccggcga
     4141 acgctccgtc ctttatcagg atggtctccc ctttctcgaa ggatatctcg tgcactttct
     4201 ttatctcgcc gtcctgcatc atcttgcgga tccgttctat ctcgaccgcc gtgatgggcg
     4261 tcggcttgat ccccgcgttc ttgtcgaaac gcttcttctc tccgacgaaa gccggtttac
     4321 ggatgaaagc gacgctcttt atgaggagcc aggcctcatc agagtaatgc atctggataa
     4381 agacataacc ggggtagagg ttcttcctca ccttgcgctt cttgtccccc ttcttttcct
     4441 ctatctccac ctcgggaact atgatctcgc cgaaatggtc ctgcattttg cgatcttcga
     4501 cgatcttcag gatcgcgttg cggagtttgt tctcctgccc cgattgggta tggatcgcgt
     4561 accatttcat gttataaccc ttccggtcgt ccctcagtaa atatacagca tgacattcgc
     4621 ccagatcaga tcgagcacga agaaaacgac gatcatgatg ccgacgaaca cgaagaccga
     4681 tatggtcgtg acccgggtct catccttggt gggcgtcgtc actttcttga gttcctcgat
     4741 cacctcatca agttgcgaac ggtaggttcc ctgccggtat gcccacagga accaggtcgt
     4801 cgcgaccgca atgacgatgc ccacgacatt cttgagcggc agaaagcccg ccagcaggta
     4861 gtcagcaagg tctatcgaac gaatgctgag caccgcatcg ctgagcgaaa cggcaaaata
     4921 ggtcatgagc gcgaaaaata ccgcgtagga aacaaggatg atcttgctat ttttcatggc
     4981 cacagctccc cgatccatcg ttcaccgtca caaaatggca ggccaggcag gactcgaacc
     5041 cgcagccctc ggttttggag accgatgctc taccaattga gctactgacc tgcatgcgca
     5101 ccgtcatatc ttgccctctt tatgctcggt atgcttgcgg tcgtgcgggc agtacttttg
     5161 gagaacgacc ttatccgccg tgttcttctt attcttggtc gtcgtgtaat tcttgcgttt
     5221 gcactccgag cagaccaagg tcaacttgat gcgatttccc gccttttttt tggaagccat
     5281 gttcgtatcc tcttgttact cgattatctt tgtcaccacg ccggcgccca ccgtgcggcc
     5341 gccctcccg
//
LOCUS       DAOQUE010000164         5347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_16925_length_5347_cov_4.707105, whole genome shotgun sequence.
ACCESSION   DAOQUE010000164 DAOQUE010000000
VERSION     DAOQUE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16925_length_5347_cov_4.707105"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..416
                     /locus_tag="PKH10_06235"
     CDS             117..416
                     /locus_tag="PKH10_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27758.1"
                     /translation="MRTARSVACVWRSEASGRTTLAPFVGALRGTEFSLLTAGRSVRF
                     TARNQLSGLGEIYIGVKAKGFLPAKEVLKEEEEAKKKAEESKKKEEEKSAEPVTP"
     gene            413..1123
                     /locus_tag="PKH10_06240"
     CDS             413..1123
                     /locus_tag="PKH10_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT27759.1"
                     /translation="MIRYGIALAWTFLYSVFGHFVWIVHLFVPGGLSQWFFRNYGRGL
                     FALCGIRLTVEGLEHIDRSRRYLITANHNSLFDIPALMATLPLDVKYFAKRELARVPV
                     FGPMLVLYRHVLVNRESRRGAVQALEAAKKTLARWSLGIFPEGTRSLDGKVHQFKTGG
                     LTLAAEAGVPVLPVAIEGTGRLLPKGALEVRSGTIHIKVFPPVPPEEARDRKQLAERL
                     ETTIRSFIETDTHGGSNV"
     gene            1116..2447
                     /gene="lat"
                     /locus_tag="PKH10_06245"
     CDS             1116..2447
                     /gene="lat"
                     /locus_tag="PKH10_06245"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HNT27760.1"
                     /translation="MSEKRSLTPRQVHAVLDTYILADGYPMVVDLMKSRGSYMYDARN
                     RRTYLDFFSFFASMPIGYNHPKTIKGKYVGDLFFSALHKPSNSDFYTVAYAEFVRTMG
                     EIAMPKWARKMFFIDGGALAVENALKTAFDWKVRKNFAKGVKKELGTKVLHFKQAFHG
                     RTGYTLSLTNTADPRKTMYFPKFDWPRFDNPKLVFPLTPANIKDAAAREAKVIRDIEA
                     VCKKDGADIAALIIEPIQAEGGDNHFRAEFLRELRRLADKYDFLLVYDEIQTGCGLTG
                     KMWAYEHFGVAPDILCFGKKFQTCGIMVGDRVLSVPKNVFAEESRINSTWGGNLVDMV
                     RSSQYLKIIRDEKLVDNAAKMGKVLQAGLMTLAKKHDAVTNVRGRGLFCAFDLPDRDM
                     RNEFLELCREKERLIVLPCGERSVRFRPFLDVDEVAIADGMERMEKVLKKL"
     gene            complement(2501..3094)
                     /gene="coaE"
                     /locus_tag="PKH10_06250"
     CDS             complement(2501..3094)
                     /gene="coaE"
                     /locus_tag="PKH10_06250"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNT27761.1"
                     /translation="MGNKLLAITGNIATGKSYVAEILRRDHDFAHVNADEIGHRVLRE
                     PPVRDAIREAFGDTALSRGEVDRRALGAIVFGDPKKLATLGDITHPRIIGIARAEVAA
                     LIEAGHRVLFEAAVLFEAGWDREFSTILLTVCHETEQIDRVMRRNACSMDEAITVIRS
                     QIPQDEKLDRATYVIDTTHGPRAFKRTLELIVKDITG"
     gene            complement(3084..3374)
                     /locus_tag="PKH10_06255"
     CDS             complement(3084..3374)
                     /locus_tag="PKH10_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27762.1"
                     /translation="MHRIAPFILVALLLGPLSCEIKEKLPDDAVVLEQIIFIDEVQQG
                     IPQTIQMPNGTVVTYKMPVKLADGDIIKIREMPGEPPFYIKVKLQQRGAHGK"
     gene            3527..4363
                     /locus_tag="PKH10_06260"
     CDS             3527..4363
                     /locus_tag="PKH10_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27763.1"
                     /translation="MRKIIALLGVSLLVAFSCSAKHEAQKTIDKYYDLIKAKSYEAAW
                     EMVDEESKELVEQETWLNDVRAVNSVSMSLKMLPAQMDKEGTRAVVMTEYTGALAARN
                     KMPDAQIRFYAVKAGDKWGINLQDRIEEIKKQRAYEAIEIPIDDTLIKTAQQYKDKII
                     VENVRNGAVQYENGLEQYMMDATITNKSDKPFSYIGVAVKFMDDTNTKVLMDKVFFLI
                     YTRQVQGIYPLKPGEKRDVIIPGYAADDIFERTGVEWTGKLQWEVEAVKLATPEELIE
                     LE"
     gene            4364..4966
                     /locus_tag="PKH10_06265"
     CDS             4364..4966
                     /locus_tag="PKH10_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27764.1"
                     /translation="MNLKDKIEEVAILVQRLDRAFTFYFTGFEKKPPLKMYERLQREV
                     KKLHDVVDGLKNSAERFTVQTLLSRFASYRIKWDRGMRDIEEGRIRPGAAFFGGVSKD
                     PKRLVAGEEGTGTAAPDLDEIARGAEKMERLDAQLGDAAKRYVALARAHLGKVYDPAA
                     IKQTLEASLPEVRRKYGDEVDFEVYFDGDKVRVRPRSLKG"
     gene            complement(4976..>5347)
                     /gene="mltG"
                     /locus_tag="PKH10_06270"
     CDS             complement(4976..>5347)
                     /gene="mltG"
                     /locus_tag="PKH10_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNT27765.1"
                     /translation="RDEQPRIAAVFLNRLRKGMKLQSDPTVIYGKLPAFNGNLTRTDL
                     ETDHPHNTYTRQGIPPTPIAAPGETAIRSVLEPATTDELFFVATGGGAHHFSVTYEEH
                     SAAVEHYQVKELRTPFVWKKR"
BASE COUNT         1079 a         1531 c         1713 g         1024 t
ORIGIN      
        1 aggtcgggag gaatctggca tgagtgcgtt ctttcaggag gcattggagg ttgcgtccct
       61 cgacgagatt cgttctgtac aggcggagcg gctgcggaag acggttcgcc acgtgtatga
      121 gaacagcgag gtctgtcgcc tgcgtctgga ggagcgaggc atcgggccgg acgacgctcg
      181 ctcctttcgt tggcgcgctt cgcggcaccg agttctccct gctcaccgcc ggtcggtcgg
      241 tccgcttcac ggcgcgtaac cagctgtcgg ggctcgggga gatatacatc ggggtgaagg
      301 ccaagggctt cctgccggcg aaagaggtct tgaaggaaga ggaggaggcg aagaagaaag
      361 cagaggagtc gaagaagaag gaggaagaaa aaagcgcgga gccggtgacg ccatgatacg
      421 ctacggcatc gcgctcgcat ggacctttct ctattcggtg ttcggtcatt tcgtctggat
      481 cgtccacctg ttcgttcccg gcgggctgtc gcaatggttc ttcaggaatt acgggcgggg
      541 gctcttcgcc ctctgcggca tccggctcac ggtcgagggt ctcgaacaca tcgatcgttc
      601 gcgacgctac cttataaccg cgaaccacaa cagtcttttc gacataccgg cgctcatggc
      661 gaccctgccg ctcgatgtga agtatttcgc gaaacgcgaa ctggcacgcg tcccggtgtt
      721 cggtccgatg cttgtcctct accggcatgt gctcgtgaac cgcgagagcc gccgcggcgc
      781 ggtgcaggcg ctcgaagcgg cgaaaaagac gctcgcccgg tggagtctcg gcatattccc
      841 cgaagggacg cgcagtctcg acgggaaggt ccaccagttc aagaccggcg gcctgacgct
      901 cgccgccgag gcgggggtcc cggtcctgcc ggtcgccatc gaggggaccg gccgcctgtt
      961 acccaagggg gcgcttgagg tccggagcgg cacgatacac atcaaggtgt tcccgccggt
     1021 gccccccgag gaggcgcgcg accgcaaaca gttggcagaa cggctcgaaa cgacgatccg
     1081 ctcattcata gaaaccgata ctcacggagg cagcaatgtc tgaaaaacgg tcccttaccc
     1141 cgcgtcaggt gcacgcggtg ctcgacacgt acatcctcgc cgatggttat ccgatggtgg
     1201 tcgacctgat gaaaagccgc ggcagttata tgtacgatgc ccggaaccgc cggacctatc
     1261 tcgatttttt ctcgttcttc gcgtcgatgc ccatcgggta caaccacccg aagaccataa
     1321 agggaaagta tgtcggcgac ctgttcttct cggcgctcca caaaccgtcg aacagcgatt
     1381 tctacaccgt cgcctacgcc gaattcgtcc ggaccatggg cgagatagcg atgccgaagt
     1441 gggcgcgcaa gatgttcttc atcgacggcg gcgcgctcgc ggtcgagaac gccctcaaga
     1501 cggcgttcga ctggaaggtc cgcaagaact ttgcgaaagg cgttaagaaa gaactcggga
     1561 cgaaggtact ccatttcaag caggcgttcc acggccgcac cggctacacg ctctcgctca
     1621 ccaataccgc ggatccgcgt aaaacgatgt atttcccgaa gttcgactgg ccccggttcg
     1681 acaacccgaa actggtgttc ccgctcacgc cggcgaacat caaggacgcc gcggcccgcg
     1741 aggcgaaggt gatccgtgac atagaggcgg tctgtaaaaa ggacggagcc gacatcgccg
     1801 cgctcatcat cgagccgata caggcggaag ggggcgacaa ccatttccgc gccgagttcc
     1861 tgcgcgaact gcggcggctc gccgataaat acgacttcct gctcgtgtac gacgagatac
     1921 agaccggatg cgggctcacc ggcaagatgt gggcgtatga gcatttcggt gtcgcgcccg
     1981 acatactctg ttttggcaag aagttccaga cctgcggcat catggtcggc gaccgggtgc
     2041 tgtcggtgcc caagaatgtg ttcgcggagg agtcgcgcat caactcgacc tggggcggca
     2101 atctcgtcga tatggtccgg tcgtcgcagt atctcaagat aatccgcgac gagaaactgg
     2161 tggataacgc cgcgaagatg ggcaaggtgt tacaggcggg gctcatgacg cttgccaaga
     2221 agcacgatgc cgtcaccaat gtccgcggtc gcggcctgtt ctgcgcgttc gacctgccgg
     2281 atcgcgatat gcgcaacgag ttccttgaac tctgccggga aaaggagcgt cttatcgtcc
     2341 tgccgtgcgg cgagcgatcg gtccgtttcc ggcccttcct cgatgtcgat gaggtcgcca
     2401 tcgccgacgg catggagcgg atggaaaagg tgctgaaaaa gctctgaatt catcccgtcg
     2461 ttcggcaccc ctctccgggc aagagaggga cgggggtgag ttatcccgtg atatctttga
     2521 ctatcagttc cagcgtcctt ttgaaggcgc gtggcccgtg ggtcgtgtct atcacatagg
     2581 tggcgcggtc cagtttctcg tcctgcggta tctgcgaccg tatcacggtg atcgcctcgt
     2641 ccatcgaaca ggcgttgcgc cgcatcacgc ggtctatctg ctccgtttcg tggcagacgg
     2701 tcagcagtat cgtggagaat tcgcggtccc agcccgcctc gaacaggacc gccgcttcga
     2761 acagcacccg atgccccgcc tcgatgagcg ccgccacctc ggcgcgggcg ataccgatga
     2821 tacgtgggtg ggtgatatcg ccgagcgtcg ccagtttctt cgggtcgccg aacacgatcg
     2881 cgccgagtgc ccgacggtcc acctcgccgc gcgaaagggc cgtgtcgccg aacgcttcgc
     2941 gtatcgcgtc gcggaccggc ggttcgcgca ggacccggtg gccgatctca tcggcgttga
     3001 catgggcgaa gtcatggtca cgccgcagta tctcggcgac ataactcttg ccggtcgcga
     3061 tgttgccggt gatggcgagc agtttatttc ccatgggctc cccgctgttg cagtttcacc
     3121 ttgatgtaaa aagggggttc tcccggcatc tcccgtatct tgatgatgtc gccgtcggcg
     3181 agtttcaccg gcatcttgta ggtgaccacc gtgccgttgg gcatctggat ggtctgaggg
     3241 atgccctgct ggacctcatc gatgaatatg atctgttcga gcacgacggc gtcatcaggc
     3301 agtttctcct ttatctcgca ggagagcggg ccgaggagca gggcgacgag gatgaatggc
     3361 gcgatacggt gcatggcggg cctctttccg tggtgtatgg tcggcccatt ataggacggt
     3421 cgcggaaaga ccgcaaattt tattgacttg acttttcgtc aatcttatac tgacccccat
     3481 gcaaggcgtt ggcgatcaca acacatatcc acgaaggggt tctacaatga gaaagatcat
     3541 cgcgttactg ggcgtttcgt tgctcgtcgc gttctcctgc tcggcgaagc atgaggcgca
     3601 gaagacgatc gacaagtatt acgatctcat caaggcgaaa agttacgaag cggcatggga
     3661 gatggtcgac gaagaatcga aggaactggt ggaacaggag acctggctga atgatgtgcg
     3721 cgccgtcaat tcggtctcga tgtcactcaa gatgttgccc gcccagatgg acaaggaggg
     3781 aacgcgcgcg gtcgtcatga ccgaatacac cggcgctctg gcggcccgca acaagatgcc
     3841 cgatgcgcag atacggttct atgccgtcaa ggccggcgac aaatggggca tcaacctgca
     3901 ggaccgcatc gaggagatca agaagcaacg tgcctacgag gcgatcgaga tcccgataga
     3961 cgatacgctc attaagacgg cccagcagta caaggacaag atcatcgtag agaatgtccg
     4021 caacggtgcg gtccagtacg agaacgggct cgaacagtat atgatggacg ccaccatcac
     4081 caacaagagc gataagccct tctcctacat cggcgtcgcg gtgaagttca tggacgacac
     4141 caacaccaag gtgctgatgg acaaggtctt cttcctcatc tacacccggc aggtgcaggg
     4201 gatatacccg ctcaagccgg gcgagaagcg cgatgtcatc atccccggtt acgcggctga
     4261 cgatatcttc gagaggaccg gcgtggagtg gaccggcaaa ctccagtggg aggtcgaggc
     4321 ggtcaaactc gccacccccg aggaactcat agaactcgaa tagatgaacc tcaaggacaa
     4381 gatcgaagag gtcgcgatac tcgtccaacg gctcgaccgg gcctttacct tctacttcac
     4441 cggtttcgag aaaaaaccgc cgctcaagat gtacgaacgg ctccagcgcg aggtgaagaa
     4501 actgcacgat gtggtggacg gcctcaagaa ttcggccgaa cggttcacgg tgcagacgct
     4561 tctgtcgcgt tttgcctcct accgcatcaa gtgggaccgc gggatgcgcg acatcgagga
     4621 ggggcgcatc cggccgggcg cggcgttctt cggcggggtc agtaaggacc cgaagagact
     4681 cgtggcgggc gaagagggga cggggacggc ggcccccgac ctcgacgaga tagcccgggg
     4741 tgccgagaag atggagcggc tcgatgcgca actcggcgac gcggcgaaac ggtatgtggc
     4801 gcttgcccgc gcccatctcg gcaaggtgta cgaccccgcc gcgataaagc agacgctcga
     4861 ggcgagtctg cccgaggtgc gccgcaaata cggcgatgag gtcgatttcg aggtctattt
     4921 cgacggcgac aaggtgcgcg tcaggccgag gtcgttgaag gggtaggcgg tccgcctacc
     4981 gttttttcca gacgaacggg gtccggagct cctttacctg atagtgctcg accgcggcgc
     5041 tgtgttcctc gtaggtgacc gagaagtggt gcgcgccgcc gccggtcgcg acaaagaaaa
     5101 gttcgtcggt ggtggccggt tcgagaacgc tccggatggc tgtttcgccg ggggcggcga
     5161 tcggcgtcgg cggtatcccc tgccgcgtgt aggtattgtg ggggtggtcg gtctcgagat
     5221 cggtgcgggt cagattgccg ttgaaggcgg ggagtttgcc gtatatcacg gtcgggtcgg
     5281 actgcagttt catccctttg cgcaggcggt tcaggaacac ggcggcgatg cgcggctgtt
     5341 cgtcgcg
//
LOCUS       DAOQUE010000165         5250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_17360_length_5250_cov_4.477767, whole genome shotgun sequence.
ACCESSION   DAOQUE010000165 DAOQUE010000000
VERSION     DAOQUE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17360_length_5250_cov_4.477767"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..248)
                     /locus_tag="PKH10_06275"
                     /pseudo
     CDS             complement(36..248)
                     /locus_tag="PKH10_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            408..1328
                     /locus_tag="PKH10_06280"
     CDS             408..1328
                     /locus_tag="PKH10_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNT27766.1"
                     /translation="MTAAVRRFMPVEPGGKIRVVATSSPFDTTLFNTGISFLERHGMR
                     PAYDDAVFARTGYLAGEPERRVAQMRDAFRDPESEAVWTARGGYGALQMLPLLTPHVA
                     EIRKHPKVFIGFSDATAIHSFLIDRCGFVTVHGPNITTLGQIERSSQRQVVRLLTGEP
                     DALRIAARQMITVRPGKAEGVVKGGNLSTLASLVGTPWEPDLKGSILFLEDVAEVPYR
                     VDRLVTQLRLAGKFAGVRGLVLGDFTYNDRRAERSVDPAMIVASSGLPASVPVMANFP
                     AGHGVKNMAFLLGAVATLDTGAKTLSYQVR"
     gene            complement(1333..3096)
                     /locus_tag="PKH10_06285"
     CDS             complement(1333..3096)
                     /locus_tag="PKH10_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27767.1"
                     /translation="MRSWTWLMLAFLCLPLFSLQAQDTAEEDAAESEEEAEEETAAVE
                     PAPASEPAPATEPAPVPKSEPAPAVAEEPAPEEGSAKVLPPKGDATEEEAVVAAEPPA
                     EQTDGLKVSGGKPIKFFELYGFFSVNHFFTYNLFMNGNNPYLPTTPLANDTERGEKIL
                     SWAMLKLHLEPIINVSETMEIHSKLTLMGSRLIGTDDAVAQTQENGLLPGTQWAVDPS
                     IRVEGLWGTLDTPIGELRVGRMPFHWGLGILYNDANRVDTDTPGDYLDRVLFLVPIMG
                     FKIIPFFDYISEGTMVRYNDHFIDASSRDDGWQLGLMFTMQEDDPAVLEEKLLKENTV
                     YEFGALLMYAWKSDASTLIDAGTDTERYVLTEQDSRIFTFDIWGNLYYKNFSLKAELA
                     FLDGKLGRYQTADLAGRIDAQMFGAALEAQWKYIPRKLHFTLLGGVATGDKASGVQGD
                     SVNLPGNAIDAALGLDTKVENFRFHRDYNFNSFIWREALGRFTSGYYASFDTQYYFLD
                     EISMRGGLTYSGAWFAKQTPGGKGTALALEPYLGATYEGESGLRAGLTFQIAVPFDGL
                     NTVARNTTVLFDIHTYMGFVF"
     gene            complement(3128..3553)
                     /locus_tag="PKH10_06290"
     CDS             complement(3128..3553)
                     /locus_tag="PKH10_06290"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27768.1"
                     /translation="MRVEQISVLLRNEPGVLSKILESLSRAQVNILGLTINETANFGE
                     LRIVTDKTAQAKEILREMAVSYNVVKIIVVRLDHKPGELLKIAHLMSRNDVNIDYIYT
                     LASADKGALIAVKTWNMAETERILEENHFEMIDLEKFAF"
     gene            complement(3666..5207)
                     /locus_tag="PKH10_06295"
     CDS             complement(3666..5207)
                     /locus_tag="PKH10_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27769.1"
                     /translation="MKKTLIGGGLAAPILLLAIATIISCDSSKPDQDADATGHDGDIV
                     ADKEYSLVDQDEVVVESEVIPDDSVLVPDSDLDTDCPPLIEAQFPFYKDDGTIHFCRP
                     GCDTPTEKDPQCVTNLWKEANLKLATDHPEADCYPYPCEMSNLKPMTKEEVESQFTTI
                     PMHECDLKLGSAGWDTDSTSGQIKHWNLSGGKAGFVMYKVSLDNRVYPTFKRYFTYDP
                     ATKKYTVQSPGDYGFVYHQGSALLYFRDARSHELSGNHYIGYFSSNGAYRVVYPKPAL
                     YIIYTPTLNEKWIFANIQETQNGPTKMMYAKAGEWKWTALGTGVAYYPDLKGDLLSFY
                     DSTEKGFLCDLSKKPASVDSCRKVNREGEVARDIVFDKENDGIAAYTNTNKVFLLRYD
                     KDPIEYTELFSDYTGKSDQFLGFIVNMLRGDMLMYTEAYFYGGSTYGGRLCFYRLDTK
                     QRSCMNKMDKDKLYGEDVVYPYGDAEFEGDHVLYQQLNSSPLILRDMKCYCEKEGVCP
                     FEGMK"
BASE COUNT          997 a         1503 c         1558 g         1192 t
ORIGIN      
        1 ggacggcgcc ggccccgcgg cccgccctat tgagccgcgc ccgaggcgcc gccttccgac
       61 tggagttcgg tcacgatgac ccgttcaccg aagatgttct cgcgcccgta agccgaccac
      121 tcgtcggcat attcgcccat gttggaactg ggtgtgatgg gataaatggc cgccacctcg
      181 gtcagcgcgt aactcgcatg ggtcgccgcc atgttgccgt caatggcctt catgatcttt
      241 tgtgacatgt attcctcctg tggaaaaaag gttatgtgta cggcgcctca tatcacgaag
      301 ggcgtatgtt tgcaaatttt tagggccccg ctattttttc gatgtaatgt ggaaaaaccc
      361 ttcgaatatg ctatagaatg tgacgactgt gacagggagg tgccgtgatg acggcggccg
      421 taagaagatt catgccggtc gagcccggcg ggaagatccg tgtggtggcg acctcgtcac
      481 cgttcgatac gacgcttttc aacaccggta tctcctttct ggagcgccac gggatgcgcc
      541 cggcatacga tgacgcggta ttcgcgcgga ccggctatct cgccggtgaa ccggagcggc
      601 gggtcgccca gatgcgtgac gccttccgcg atccggagtc cgaggcggtc tggaccgcgc
      661 gtggcggcta cggcgccctg cagatgttgc cgctcctcac cccgcatgtc gccgagatac
      721 ggaaacaccc caaggtattc atcggttttt ccgacgcgac ggccattcac tcgttcctga
      781 tagaccgttg cgggttcgtg acggtgcacg gcccgaacat cacgacgctc ggccagatag
      841 agcgttcttc ccagcgacag gtggtccgcc tcctcacggg cgagcctgac gcgctccgta
      901 tcgcggcgcg gcagatgata accgtaaggc cgggaaaggc cgaaggggtg gtgaaggggg
      961 gtaatctctc gacgcttgcc tcgctcgtcg gcacaccgtg ggaacccgat ctcaagggga
     1021 gcattctctt ccttgaggat gttgccgagg tgccctaccg cgtcgatcgg ctcgtcacgc
     1081 agttgcggct cgccggtaaa ttcgccgggg tacggggtct cgtgctcggc gatttcacct
     1141 ataacgaccg ccgggccgaa cgaagcgtgg acccggcgat gatcgtcgcg tcgagcgggc
     1201 ttcccgcctc ggtgccggtc atggcgaact tccccgccgg gcatggcgtg aagaacatgg
     1261 cctttctgct tggcgccgtc gccacactgg ataccggggc gaagacgctt tcttatcagg
     1321 tccgctgata ccttagaaca cgaaacccat gtaggtgtgg atgtcgaaca ggacggtggt
     1381 gttgcgcgcc acggtgttga gcccgtcgaa gggtaccgcg atctggaagg tgagtccggc
     1441 gcgcaggccg ctctcacctt cgtaggtggc gccgaggtac ggttcgagcg cgagcgccgt
     1501 tcccttaccg ccgggggtct gcttggcgaa ccacgcgccc gagtaggtca gaccgccgcg
     1561 catgctgatc tcgtcgagga aatagtactg ggtatcgaaa ctcgcgtagt agcccgaggt
     1621 gaaccggccg agggcctcgc gccagatgaa cgaattgaag ttgtagtcgc ggtggaaccg
     1681 gaaattctcg accttggtat cgagtccgag cgccgcgtct atcgcgttcc cgggcagatt
     1741 caccgaatct ccctgcaccc cgctcgcctt gtcaccggtc gccacgccgc ccagcagggt
     1801 gaaatggagt ttgcgcggga tgtatttcca ctgcgcctcg agcgcggcgc cgaacatctg
     1861 ggcgtcgatg cggccggcga ggtcggccgt ctggtagcgc cccagtttcc cgtcgaggaa
     1921 cgccagttcg gccttcagcg agaagttctt gtagtacagg ttgccccaga tgtcgaacgt
     1981 gaatatgcgg gaatcctgct cggtcagcac atagcgttcg gtgtcggtgc cggcgtcgat
     2041 cagcgtcgag gcgtcgcttt tccacgcgta catgaggagc gcaccgaact cgtagacggt
     2101 attctccttg agcagtttct cctcgagcac cgccgggtcg tcctcctgca tcgtgaacat
     2161 gagtcccagt tgccagccgt cgtcgcgcga ggaggcgtcg atgaagtggt cattgtagcg
     2221 gaccatcgtc ccttcgctga tgtagtcgaa gaagggtatg atcttgaatc ccatgatcgg
     2281 cacgaggaac aggacgcggt cgagatagtc gcccggggtg tcggtatcga cgcggttggc
     2341 gtcgttatag agtatgccga gtccccagtg gaacggcatg cgccccacgc gcagttcgcc
     2401 gatgggggtg tcgagcgtcc cccagagtcc ctcgacccgt atcgagggat cgaccgccca
     2461 ctgcgtaccg ggaagcagtc cgttctcctg tgtctgcgcc accgcgtcgt cggtgccgat
     2521 gagccgactc cccatgaggg tgagtttcga gtgtatctcc atcgtctcgg atacattgat
     2581 gatcggttcg aggtgcagtt tcagcatggc ccacgagagt atcttctcgc cgcgttcggt
     2641 gtcgttggcg agcggggtgg tcggcaggta ggggttgttg ccgttcatga aaaggttgta
     2701 ggtgaagaag tgattgacgc tgaaaaagcc gtagagttcg aagaatttta tcggcttgcc
     2761 gccgctcacc ttcagtccgt cggtctgttc ggcgggcggt tcggccgcga cgacggcctc
     2821 ttcctcagtg gcgtcgccct tcggcggcag gaccttcgcg gatccctcct ccggggcggg
     2881 ctcttcagcg accgcggggg ccggttcaga tttgggaacc ggcgcaggtt cggtggccgg
     2941 cgcaggttcg gaggccggtg ccggttcgac agcggcggtc tcctcttcgg cctcctcttc
     3001 gctttcggcg gcatcttcct ccgccgtgtc ctgcgcctgc agggagaaga gcgggaggca
     3061 gaggaacgcc agcatcagcc atgtccatga acgcatatga cctcctttcg ggatatccgg
     3121 gcagtgatta aaacgcgaat ttctcaagat cgatcatctc gaagtggttc tcttcaagta
     3181 tccgttcggt ctcggccatg ttccaggtct tgacggcgat gagcgccccc ttgtcggccg
     3241 aggcgagcgt gtagatgtag tcgatgttca catcgttgcg gctcatcagg tgggcgatct
     3301 tcagcagttc gcccggcttg tggtcgagcc gcaccacgat gatcttcacc acattgtagg
     3361 aaacggccat ctccctgagt atctccttgg cctgcgccgt cttgtcggtg acgatgcgca
     3421 gttcgccgaa attcgccgtt tcgttgatgg tgaggcccag tatgttgacc tgcgcgcgac
     3481 tgagactttc gagtatcttg ctgagcactc ccggctcgtt gcgcaacagg accgagatct
     3541 gttccacccg catggtttcc tcccttacaa agcgtgtcgc gtcaccatag cattataccg
     3601 gggaggtgtc aaaaaaattc aggaaggatg ggggaggggg agcggttagg gccggcggcg
     3661 gtcgatcact tcatcccctc gaacgggcag acaccttctt tttcacagta acacttcatg
     3721 tcgcggagaa tgagcgggga actgttcagt tgctggtaaa gaacatgatc cccctcgaac
     3781 tcggcatcgc catagggata gaccacatcc tctccgtaca gtttgtcttt atccatttta
     3841 ttcatacagg aacgctgttt cgtgtcgagg cgatagaagc ataaccgccc gccgtaggtg
     3901 ctcccgccgt agaaatacgc ctcggtgtac atcagcatat cgccccgcag catattcacg
     3961 atgaagccga ggaattggtc agatttgccg gtgtagtcgg aaaacaattc cgtatattcg
     4021 atggggtctt tgtcgtagcg caagagaaaa actttattcg tgttcgtgta ggcggcgatc
     4081 ccgtcattct ctttgtcgaa aactatgtcc cgcgccacct ctccttctcg gttcaccttc
     4141 cggcaggaat caaccgaggc cggttttttc gaaaggtcac acaggaatcc tttttcggta
     4201 ctatcgtaga aagagagaag gtctcccttc aggtcgggat aatacgccac gcccgtcccc
     4261 agcgctgtcc atttccactc gcccgccttc gcgtacatca tcttcgtggg accgttttgt
     4321 gtctcttgga tgttggcaaa aatccatttt tcgttgagcg ttggtgtata aatgatgtag
     4381 agcgctggct tgggatacac cactcggtaa gcgccattgg aagaaaaata accgatataa
     4441 tgattgccgc tcaactcgtg agagcgggca tccctgaaat acagaagtgc gcttccttga
     4501 tgatatacaa aaccataatc tcccgggctc tgaacggtgt atttcttcgt tgcagggtcg
     4561 taggtaaaat atctcttaaa ggtggggtac acccgattgt ccagcgatac cttatacatc
     4621 acaaaccccg cctttccccc gctcagattc caatgcttga tctggccact agtggaatcg
     4681 gtatcccatc cggcagaccc taatttaagg tcgcactcat gcatggggat ggtcgtgaac
     4741 tgcgattcaa cctcttcctt cgtcatcggc ttcagattac tcatctcaca cggataggga
     4801 taacaatctg cctcaggatg atcggtggca agtttcagat tcgcctcttt ccaaagattt
     4861 gtgacacatt gcgggtcctt ctcggtcggg gtgtcacacc cggggcggca gaagtgaatg
     4921 gtgccgtcat ccttgtagaa ggggaactgc gcctcaatga ggggggggca gtcggtgtca
     4981 agatcgctgt cgggcacaag aacagagtcg tccggtatca cctcgctctc aacaacgacc
     5041 tcgtcctgat cgacaagaga gtattctttg tctgccacga tatctccgtc atgccccgtc
     5101 gcgtcggcat cctgatctgg tttcgacgag tcacaagaga ttatcgtcgc tatagccaac
     5161 aagagtatgg gggcggctaa gccgcccccg ataagtgttt tcttcattct ttagcaccca
     5221 ctccagtaac agtctcccgt atcataaccg
//
LOCUS       DAOQUE010000166         5247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_17374_length_5247_cov_4.094183, whole genome shotgun sequence.
ACCESSION   DAOQUE010000166 DAOQUE010000000
VERSION     DAOQUE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17374_length_5247_cov_4.094183"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="PKH10_06300"
     CDS             complement(<1..1772)
                     /locus_tag="PKH10_06300"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNT27770.1"
                     /translation="MKRLTTMLTMSLLPLLATASEADLVIPNLTPAQNNLLLLGFVVC
                     FLGLGFGLWQYLKVKNIRAHKAMLDVSQTIFETCKTYLLQQGKFLIILEVLIGICIAF
                     YFGYLQHNGFGTVLLILLWSVVGILGSYGVAWFGIRMNTLANSRMAFSALERKPIKLL
                     NIPLDAGMSIGVVLVCVELIMMLIILLFVSREAAGACFIGFAIGESLGASALRVAGGI
                     FTKIADIGSDLMKIVFKIKEDDPRNPGVIADCTGDNAGDSVGPTADGFETYGVTGVAL
                     ISFIVLAVADVSQQATLLTWIFVMRILMVITSIVAFWLNRAITAATQSGKDDIDFEHP
                     LTVLVWVTSIVSILVTFAASYVMIGDLPNNLWITLSVIISCGTLAAAVIPEFTKIFTS
                     PKSKHVLETVNASREGGASLNVLSGLVAGNFSAFWQGMVFFALMFAAYYASGFGLGEL
                     MIFPSIFAFGLVAFGMLGMGPVTIAVDSYGPVTDNAQSVYELSLIETMPGIDKEIEKD
                     FGFKPDFEKAKYYLEANDGAGNTFKATAKPVLIGTAVVGATTMIFSLILVIRNTLGID
                     PQVILSELNPYTILGFICGGAVIF"
     gene            1952..3607
                     /gene="pgi"
                     /locus_tag="PKH10_06305"
     CDS             1952..3607
                     /gene="pgi"
                     /locus_tag="PKH10_06305"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNT27771.1"
                     /translation="MFPRSNPRLTAAWRELEAHAATMSTVSLRDLFVDDPSRAGRFSR
                     WLEDILVDCSKNIITDETLRLLCALAAECGVKEARDAMFAGERINETEGRAVLHVALR
                     NRSDRPMTVDGVDVMPAVKRVLGQMRDFSEQVIGGAWKGYSGRCITDIVNIGIGGSDL
                     GPVMVCAALRPYWKGGITPHFVSNIDGTHIIETLKGLNPETTLFIVASKTFTTQETMT
                     NAETARRWLLDKAKDEKAVARHFVAVSTNAKEVARFGIDTANMFEFWDWVGGRYSLWS
                     AIGLSIACTIGADRFEELLAGAHAMDEHFRVEPFERNIPVILALLGVWYNNFFGAHSE
                     AILPYDQYLHRFAAYFQQGNMESNGKAVDRNGLPVTYETGPIIWGEPGTNGQHAFYQL
                     IHQGTRLIPCDFIAPAISHNPVGDHHRKLLANFFAQTEALMMGKTADEVRAEMERAGK
                     SADEIERVLPFRVFSGNRPTNSILCHKIDPRTLGILIAMYEHKIFTQGVIWNIYSFDQ
                     WGVELGKQLANRILPELEGDEPVTGHDCSTLGLINAYKQLRKG"
     gene            3614..4180
                     /locus_tag="PKH10_06310"
     CDS             3614..4180
                     /locus_tag="PKH10_06310"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT27772.1"
                     /translation="MRPFLLLLLAPLFLAAADTSYDLTDGTVLDRATGLRWQRGTADR
                     PMTYRDAERYCRDLSLEGRDDWRLPELSELRTLIVGCPGSRPGGACPVDEGSGGCGGD
                     GCLHSKCYDKNNCACAEGKGPGEGGRYQATGVWRGAESRFWSATGRDDCPGSSWRVNF
                     LKGAVGYDERNNLNAVRCVREERNGAKR"
     gene            4177..5019
                     /gene="nfo"
                     /locus_tag="PKH10_06315"
     CDS             4177..5019
                     /gene="nfo"
                     /locus_tag="PKH10_06315"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNT27773.1"
                     /translation="MKYLGAHVSAAGGVEMAPRNAHAIGATAFALFTRNQKQWSPKPL
                     TAENIAAFRAACVELNFGPDQILAHDGYLINLGHPEEEGLEKSRAAFLDEMKRCELLG
                     IELLNFHPGSHLGKIPEDECLSRIATSLNIAHERVPGVTTVIENTAGQGTNIGHRFEH
                     IAAIIDRVEDKTRVGVCIDTAHTYAAGYDIREAEGYERMWRDLDRIVGLRYLRGMHIN
                     DSKKALATRVDRHDHLGAGTLGIDFFRRLMRDDRLDGIPLILETPDESKWAEEIALLR
                     GFAS"
     gene            5016..>5247
                     /locus_tag="PKH10_06320"
     CDS             5016..>5247
                     /locus_tag="PKH10_06320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT27774.1"
                     /translation="MNTPLVSAVVAVHNGERYIRAAVESLLADHLRDIEVVVVEDGST
                     DGTRDILSCIDDDRLTVVDNGGNLGVATSFNRA"
BASE COUNT         1048 a         1603 c         1692 g          904 t
ORIGIN      
        1 cagaagatga ccgcgccgcc gcagatgaag ccgaggatgg tgtaggggtt gagttcagag
       61 aggatgacct gcgggtcgat gccgagcgtg ttcctgatga cgaggatgag cgagaatatc
      121 atcgtggtcg cgccgaccac cgcagtgccg atgagcaccg gcttggcggt cgccttgaag
      181 gtgttgcccg cgccgtcgtt cgcctcgagg tagtatttcg ctttctcgaa gtcgggcttg
      241 aagccgaaat ccttctctat ctccttgtct atgccgggca tcgtttcgat gagcgacaat
      301 tcatagaccg actgggcatt gtcggtgacc ggaccgtagg aatcgaccgc tatggtgacc
      361 gggcccatgc cgagcatgcc gaacgcgacg agtccgaagg cgaagatgga ggggaatatc
      421 atgagttcgc cgagcccgaa gccgctggcg taataggcgg cgaacatgag cgcgaagaag
      481 accatgccct gccagaaggc gctgaagttg cccgccacga gccccgacag cacattgagc
      541 gacgcgccgc cctcacggga ggcgttgacc gtctcaagga catgtttcga cttggggctc
      601 gtgaatatct tggtgaattc ggggatgacg gccgccgcga gcgtgccgca tgaaatgatg
      661 accgacaggg ttatccagag gttgttcggc agatcgccga tcatcacata actcgccgcg
      721 aaggtgacga gtatcgagac gatcgaggtg acccagacga gcaccgtcag cggatgctcg
      781 aagtcgatgt cgtccttgcc cgattgggtc gccgcggtta tcgcgcggtt cagccagaag
      841 gcgacgatcg aggtgatgac catgaggata cgcatgacga atatccatgt cagcagggtc
      901 gcctgttggg aaacatcggc gaccgccagc acgatgaagg atatgagcgc gacgccggtg
      961 acgccgtagg tctcgaagcc gtcggcggtc gggccgaccg aatcgcccgc gttgtcgccg
     1021 gtgcagtcgg cgatgacgcc gggattgcgc gggtcgtcct cttttatctt gaagacgatc
     1081 ttcatgaggt cggagccgat gtcggcgatc ttggtgaaga tgccgcccgc gacgcggagc
     1141 gccgaggcgc cgagcgattc gccgatggca aaaccgatga agcaggcgcc cgccgcctca
     1201 cgggagacga acagcaggat gatgagcatc atgatgagtt cgacgcagac gagcacgacg
     1261 ccgatggaca tgccggcatc aagcgggatg ttgagcagtt ttatcggttt gcgctcgagc
     1321 gcgctgaagg ccatgcggga gttggccagc gtgttcatcc ggatgccgaa ccacgcgacg
     1381 ccgtaggagc cgagtatgcc gaccaccgac cacaggagta tcaacaggac ggtgccgaag
     1441 ccgttgtgct ggagataacc gaaatagaac gcgatgcata tcccgatgag cacttcaagg
     1501 atgatgagga acttgccctg ctggagcaga taggtcttgc aggtctcgaa gatggtctgc
     1561 gagacatcca gcatcgcctt gtgggcgcgg atgttcttga ccttgagata ctgccacaga
     1621 ccgaagccga gtccgaggaa acagacgacg aacccgagca ggagcaggtt gttctgcgcg
     1681 ggggtcaggt tcgggatcac gagatcggcc tcgctcgccg tcgcgaggag cgggagcagg
     1741 gacatcgtta acatcgtggt gagccgtttc atggggtatc ctccgtggtt caagaatggt
     1801 ctcttccaaa ccgccgcaac tttagcgaaa gtcggtgtaa aaagtcaaga aaacggttcg
     1861 tgcgtcaaag aagcggcgct tccattgacc cgtcggcctt ttatcgctat agtggagcgt
     1921 ccgtgaacga ccttttgtgg agggtatgcc catgttcccg cgtagcaatc cccgcctgac
     1981 agccgcgtgg cgcgaattgg aggcccacgc cgccacgatg agcacggtct ccctgcgcga
     2041 cctgttcgtt gacgacccgt cccgcgccgg tcgattctcg cgctggctgg aggacatcct
     2101 cgtggactgc tcgaagaaca tcatcaccga cgagaccctg cgcctgctct gcgcgctcgc
     2161 cgctgaatgc ggcgtgaagg aggcccggga cgcgatgttc gcgggagaac ggatcaacga
     2221 gaccgaaggc cgcgcggtgc tccatgtcgc cctgcggaac cggtcggacc gcccgatgac
     2281 ggtggacggc gtcgatgtca tgcccgcggt gaaacgcgtg ctcggccaga tgcgcgattt
     2341 ttccgaacag gtcatcggcg gcgcgtggaa aggatactca ggccggtgca tcaccgatat
     2401 cgtgaacatc ggcatcggcg ggtcggatct cggcccggtc atggtctgcg cggcgctccg
     2461 gccttactgg aaggggggta tcacgcccca tttcgtatcg aacatcgacg gcacccatat
     2521 catcgagacg ctgaaagggc tgaatcccga gacgacgctc ttcatcgtcg cctcgaagac
     2581 cttcacgacg caggagacga tgaccaacgc cgagacggcg cgccgctggc tactcgacaa
     2641 ggcaaaggac gaaaaggcgg tcgcccggca tttcgtcgcc gtttcgacca atgcgaagga
     2701 ggtcgcgagg ttcgggatag ataccgccaa catgttcgaa ttctgggact gggtcggcgg
     2761 ccgctattcg ctctggtcgg ccatcggtct gtccatcgcc tgcacgatag gggccgatcg
     2821 gttcgaggaa ctgctcgccg gcgcccacgc catggacgag catttccgcg tggagccgtt
     2881 cgagcgcaat atcccggtca tcctcgccct gctcggcgtc tggtacaaca acttcttcgg
     2941 cgcgcacagc gaggcgatac tgccctacga ccagtacctg catcggttcg ccgcctattt
     3001 ccagcagggt aacatggaat cgaacggcaa agccgtcgac cgcaacggtc tgccggtgac
     3061 ctacgagacc gggccgatca tctggggtga gccggggacc aacggccagc acgccttcta
     3121 tcaactcatc catcagggga cgcggctcat cccgtgcgac ttcatcgccc ccgccatcag
     3181 ccacaatccc gtcggcgacc accaccgtaa actgctcgcg aacttcttcg cgcagaccga
     3241 ggcgctcatg atgggcaaga cggctgacga ggtccgcgcc gagatggagc gggccgggaa
     3301 gagcgccgat gagatcgagc gggtcctgcc gttccgcgtc ttttcgggca accggccgac
     3361 caactcgata ctctgtcaca agatcgaccc gcggacgctc ggcatcctga tagcgatgta
     3421 cgagcacaag atatttacgc aaggggtcat ctggaacatc tacagtttcg accagtgggg
     3481 ggtcgagctg ggcaaacaac tggcgaaccg gatactgccc gaactcgagg gggacgagcc
     3541 ggtcaccggt cacgattgct cgacgctggg actcataaac gcctacaaac agttgcgcaa
     3601 agggtagggg ccgatgcgac cgttcctgct cctcctgctc gcgccgctgt tcctcgcggc
     3661 cgccgatacc tcttatgacc tgaccgacgg caccgtgctc gaccgcgcca ccgggctccg
     3721 gtggcaacgt ggcacggccg atcgtccgat gacctatcgc gatgcggagc ggtactgccg
     3781 cgacctgtcg ctcgaaggcc gcgacgactg gcggctgccc gaactttccg aactgcgaac
     3841 gctcatcgtc gggtgcccgg gaagccgtcc cggcggcgcc tgtccggtcg acgaggggag
     3901 cggcgggtgt ggtggcgacg gatgccttca ctccaaatgt tacgacaaga acaactgcgc
     3961 ctgcgccgaa ggaaaggggc ccggcgaggg gggccggtat caggcgaccg gcgtctggcg
     4021 cggggcggag agccgtttct ggtccgccac cgggcgtgac gattgccccg gcagttcgtg
     4081 gcgcgtcaat ttcctcaaag gggcggtcgg atacgacgaa cgcaacaacc tcaacgcggt
     4141 gcgctgtgtc cgtgaagaac ggaacggggc gaaacgatga agtacctcgg cgcccatgtc
     4201 agcgcggcgg gcggcgtcga gatggcgccg cggaacgctc atgccatcgg ggcgaccgcc
     4261 tttgcgctct tcacccgcaa ccagaagcag tggagcccga agccgctgac ggccgagaac
     4321 atcgcggcgt tcagggcggc ctgcgtcgaa ctgaacttcg gccccgacca gatactggcg
     4381 catgacggct atctcatcaa cctcggccat cccgaggaag aaggcctcga aaagtcgcgc
     4441 gccgcgttcc tcgacgagat gaagcggtgc gaactgctcg gcatcgaact cctcaacttc
     4501 catcccggca gtcacctcgg taagataccc gaggacgaat gcctctcccg tatcgccact
     4561 tcgctgaaca tcgcccatga acgggtgccg ggggtgacga ccgtcatcga gaacacggcg
     4621 gggcagggga ccaacatcgg ccaccggttc gaacatatcg ccgcgatcat cgaccgggtg
     4681 gaggataaga cgcgtgtcgg cgtctgcatc gacaccgccc acacctacgc cgccggctat
     4741 gacatccgcg aggcggaggg gtacgagcgg atgtggcgcg acctcgaccg catcgtcggg
     4801 ctccgctacc tgcgcggcat gcacatcaac gattcgaaaa aggcgctcgc gacccgtgtg
     4861 gaccgccacg atcacctcgg cgcggggacg ctcgggatcg actttttccg ccgtctcatg
     4921 cgcgacgacc ggctcgacgg catcccgctc atactcgaaa cgcccgacga gtcgaaatgg
     4981 gccgaagaga tagcgctcct gcgggggttc gcgtcatgaa tacaccgctc gtgtcggccg
     5041 ttgtcgccgt ccacaacggc gaacgctaca tccgcgccgc cgtcgagagc ctgctcgccg
     5101 accatctgcg cgacatcgag gtggtggtgg tcgaggacgg ttcgaccgac ggcacccgcg
     5161 atatcctttc ctgcattgac gacgaccggc tcaccgttgt cgacaacggc ggcaatctcg
     5221 gggtcgcgac ctcgttcaac cgtgcca
//
LOCUS       DAOQUE010000167         5245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_17388_length_5245_cov_3.890173, whole genome shotgun sequence.
ACCESSION   DAOQUE010000167 DAOQUE010000000
VERSION     DAOQUE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17388_length_5245_cov_3.890173"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2691)
                     /locus_tag="PKH10_06325"
     CDS             complement(<1..2691)
                     /locus_tag="PKH10_06325"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27775.1"
                     /translation="MDIDGEDGLIDGSLEEDTTPSSDTDDPAPDDDTEKVACSLSFAG
                     EIGGPAFDVATAGDILLVARGQGGLYLYDASDPQYPSLLSHIQSNFIVRGICAADGIA
                     YLVGTDDSYSSGALQIVDIHDPEHPKAIGARPLTEIAFKVAVANGHAFVANSVAGLAV
                     IDVSTPSAPGEPLFVATSGSAGNVHLIDSVAYVSHENGLAIIDATDPTAPKSLFSLLV
                     PRARGLAVRNGYAYVAGDANGLTILDISSPKSPNIKKTVGAFGYAADVAIDGDHAYVA
                     SDDQGLVVYDISEPLGPRFVGWKDTPGDAVAIALSNGHAFLAADYAGVRIVDLKEVST
                     PVEVAALTAPVAAFAVKVAGDFAYVADYEGGLKVVDIRDPARPVGIAASRDIGFASWD
                     IELMGNIAVASDYYRGGVHLFDITEPADPVRTGWAVSGQSERNYGRGFSLVGDLAYLS
                     GNGLAIFDLSDPAEPTLLSATVFGNNTSDVAVRGGYAFLVDWDGLLWVADISNPYAPR
                     FVTDLQVGEDSFALTRRGDRLFVADRVSGVTIVDIADPTAPAVIRSTPLADGAKSVVF
                     KDALAFVTSNETLRVFLLDDDKLTKIAELELPDYAYGMTIDDTHAYIADSGAGLQIVR
                     YECSGVEPVEEPDDIPDDIMPDEDHELPDDVDLLPDDAPVYPCQGTILSSFGGQTVDM
                     AGRPGTLFALNSPRGIAAYDVTDPAHPAPLSFHALPFRYQAVSIAVTDHYLLAAGDYD
                     DELFLVDASDPSLLTDVGTIPIQGGPEDILVNDHYLYVSLAGYGIQILDIADPTAPVE
                     LATLALPADPGQLALDGDTLFTVTSGQLNTYEGMLAIDVSDRTDPRLVGTFFLPYAQS
                     IAAADGVVYLEAADPYNARLVTVDANDPSHSTETDRFEGEG"
     gene            2684..3094
                     /locus_tag="PKH10_06330"
     CDS             2684..3094
                     /locus_tag="PKH10_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27776.1"
                     /translation="MSTRLSSSPGVAGWLLSAQAHSKKSKSKGGSNRGRIIVLNMLAS
                     ADIYLFRFTLSAGLLIVNMGFSCFQHFGEETVKIKNGPIGKLKTPSPGRWRVHRRENL
                     FGGLCHESQENRWKKYPRKDGRLKRKRGAMLKVC"
     gene            complement(3100..3507)
                     /locus_tag="PKH10_06335"
     CDS             complement(3100..3507)
                     /locus_tag="PKH10_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27777.1"
                     /translation="MRYVIGILTVLATTALFGGGAFAEAIDKEGNSSTWTFFQKDKAG
                     AAAAQAEAIKKLKEQGHAKVSSPASTGMDHGIYVVIFTAYEHKGEARWSHGFGIHAKS
                     EKEAEKAAVANLKKKDKFWKPDYGYDVKETKNF"
     gene            complement(3574..4245)
                     /gene="queC"
                     /locus_tag="PKH10_06340"
     CDS             complement(3574..4245)
                     /gene="queC"
                     /locus_tag="PKH10_06340"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNT27778.1"
                     /translation="MKSVVLFSGGLDSTTALAIAIARGDEVHPLTISYGQRHRVEIEK
                     ARRTLDRYRLADRAREISFDLSFLHSSALTDRSLAVPRHTDDADAIPITYVPARNLIF
                     LSLAAAYAEDIGAERIIIGVNAVDFSGYPDCRPAFIEAFERAVRSGTKAGDRLAVEAP
                     LIALSKKEIVEWGVRLGVDHSLTHSCYDPASDGAACGTCDSCRLRLKGFAEAGIVDPV
                     RYVAR"
     gene            complement(4242..>5245)
                     /locus_tag="PKH10_06345"
     CDS             complement(4242..>5245)
                     /locus_tag="PKH10_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNT27779.1"
                     /translation="PHLPSPSRFRGIAIEPSLPAVTDTLILADPLLLFDGPFLRNLTV
                     NFDPPVVACDPATLEPIGFARLRAADLSDDFFDDPWPRLREFVDRRQDTRVAVIAARY
                     FAVRAVADLAAAEQYLLRSLVHKGDDIVERNLTRPISRFLTSWTANYRVSPNLWTLLA
                     MLLGITAALLPLLNGSLEGIVPPFLLCAAAIVSAVDGETARLTFRTSRQGGILNYYSS
                     RLVVWAYFLTITLLSRDIPWAYFPGIVFSFLYPLLVIRYDRYRHRAEAPGGPVRRIER
                     SIRRHFSIHTVALIVLFCAIAGVLPLAARAALPLAATGAMLAMYEAIAGKLERRKRP"
BASE COUNT         1025 a         1478 c         1799 g          943 t
ORIGIN      
        1 cccttcgcct tcgaaacgat cggtctccgt ggagtgcgag ggatcgttcg catccaccgt
       61 caccagtcgc gcgttgtaag gatccgcggc ttccagatag acgacgccgt cagccgccgc
      121 tatcgattgc gcgtagggga ggaagaaggt gccgacaagg cggggatcgg tgcggtcgct
      181 cacatcgatg gcgagcatcc cttcataggt gttcaactga ccgcttgtca cggtgaaaag
      241 ggtatcgcca tcgagggcga gttgcccggg gtcggccggc agggcgaggg tcgccagttc
      301 gaccggcgcg gtcggatcgg cgatgtcgag tatctggatg ccgtagccgg cgagagagac
      361 atagaggtaa tgatcgttca ccagtatatc ctcggggcct ccctgtatcg ggatggtccc
      421 gacatcggtc aggagagacg ggtcggatgc gtccacgaga aagagttcgt catcgtaatc
      481 tccggcggcc aacagatagt ggtccgtgac ggcgatagag acggcttggt agcgaaaagg
      541 aagcgcgtga aaggaaaggg gcgccgggtg ggcgggatcg gtgacatcgt acgcggcgat
      601 accgcgcggt gaattgagtg cgaagagggt gccgggcctg cccgccatat cgaccgtctg
      661 cccgccgaag gaggagagga tcgtgccctg acagggatag accggcgcgt cgtcgggcag
      721 gaggtcgaca tcgtccggga gttcgtggtc ctcatccggc atgatgtcgt cggggatgtc
      781 gtcgggctcc tcgaccggct cgacgccgct gcattcgtag cgcacgatct ggagtccggc
      841 ccccgaatcg gcgatgtagg cgtgcgtgtc atcgatcgtc atgccgtagg cgtaatcggg
      901 cagttcaagt tcggcgatct tcgtgagttt gtcatcgtcc agaagaaaaa cccgcagggt
      961 ctcattgctg gtcacgaagg cgagggcgtc cttgaatacg acacttttcg cgccgtcggc
     1021 gagcggcgtg ctccggatga ccgcgggggc ggtcgggtcg gcgatgtcga cgatggtcac
     1081 cccggagacg cgatcggcga caaagagacg atcgccgcgc cgtgtgagcg cgaaggagtc
     1141 ttccccgacc tgcaggtcgg tgacgaagcg gggggcgtac gggttcgaga tatcggcgac
     1201 ccagaggagc ccgtcccagt cgaccaggaa cgcgtacccg ccgcgcacgg ccacatcgct
     1261 ggtgttgttg ccgaaaactg tggcggaaag cagcgtcggc tctgccggat cgctcagatc
     1321 gaatatggcg agcccgttcc ctgacaaata agcgaggtcc ccgacgaggg aaaagccccg
     1381 tccgtaattt ctttcggatt gcccggagac cgcccaccct gtccgtacgg gatcggcggg
     1441 ctcggtgatg tcgaacaggt gaacgccgcc gcggtagtaa tcggaggcga cggcgatgtt
     1501 ccccatcagt tcgatgtccc atgaggcgaa gccgatatcg cggctcgccg ctatcccgac
     1561 ggggcgcgca gggtcgcgta tgtcgacgac ctttagacct ccttcataat cggccacata
     1621 ggcgaagtcg cccgcgacct tgacggcgaa ggcggccacc ggtgcggtca gcgcggcgac
     1681 ctcgaccggc gtgctcacct ctttcaggtc cacgatgcgg acaccggcgt agtcggcggc
     1741 gaggaaggca tgcccgttcg acagggcgat cgccacggcg tcgccggggg tgtccttcca
     1801 tccgacgaat ctcgggccaa gcggttccga gatgtcgtac acgacgagcc cctgatcgtc
     1861 gctcgccaca taggcgtgat cgccgtcgat ggcgacatcg gcggcatagc cgaacgcgcc
     1921 cacggtcttt ttgatgttcg gcgatttggg agatgagatg tccagtatcg tcaggccatt
     1981 ggcatcgccg gccacatagg catagccgtt acggacggca agcccgcggg cccgcggcac
     2041 cagaagcgag aacagggatt tgggggcggt cggatcggtc gcatcgatga tggccagacc
     2101 attctcatgg gaaacatacg cgaccgaatc gataagatgg acattcccgg cgctcccgct
     2161 cgtcgcgacg aatagcggtt ccccgggcgc ggagggggtg gagacatcta tgaccgccag
     2221 tccggcgaca ctgttcgcga caaaggcgtg gccgttcgcg accgcgacct tgaaagcgat
     2281 ctcggtcagt ggccgggcgc cgatggcctt ggggtgctcc gggtcatgga tgtcgactat
     2341 ctgcaacgcg ccggaggaat aagagtcgtc ggtgccgacg agataggcga tcccgtcggc
     2401 ggcacagatc ccccgcacga tgaagttgct ttgtatgtgg ctcaggaggg agggatactg
     2461 tggatcgctc gcgtcgtaga gatacaggcc tccctgtccg cgagcgacaa ggaggatatc
     2521 ccccgcggtc gcgacatcga acgccggtcc gccgatctct ccggcaaagg aaagggaaca
     2581 ggcgaccttt tccgtatcgt catcgggcgc cgggtcgtcg gtgtcggaag agggggtggt
     2641 gtcttcttcg agggatccgt cgatcagacc atcttctcca tcgatgtcca cgcggttatc
     2701 ttcgtcaccc ggggtggcag gatggctact ttccgcgcag gcgcatagca aaaaaagcaa
     2761 gagcaaaggc ggcagcaaca ggggaaggat catcgtcctc aacatgctcg cctccgcaga
     2821 tatctacctg tttcggttca ccttatcagc aggtcttttg attgtcaata tggggttttc
     2881 ctgctttcaa catttcggtg aggagacggt taaaatcaaa aacggtccta tcgggaaatt
     2941 aaaaacgcca tcgccggggc gctggagggt gcatcgtcgg gaaaaccttt tcggaggcct
     3001 ttgtcacgag agtcaagaaa accggtggaa aaaatatccc agaaaagatg gacggttgaa
     3061 aagaaagcgg ggggcgatgt tgaaagtttg ttgaaaaagt cagaagttct tggtctcttt
     3121 gacatcgtac ccgtagtcgg gtttccagaa cttgtctttt ttcttcaggt tggcgaccgc
     3181 cgccttttcg gcctctttct cggactttgc gtgaatgccg aacccgtggc tccaacgcgc
     3241 ctcgcctttg tgttcgtaag cggtgaagat gacgacatag atgccgtggt ccatgccggt
     3301 gctcgccggg gacgagacct tcgcatggcc ctgttccttg agtttcttga tggcctcggc
     3361 ctgcgccgcc gccgcgcccg ccttatcctt ctggaagaag gtccaggtcg aggagttgcc
     3421 ctccttgtct atcgcctctg cgaaagcgcc gccgccgaaa agcgcggtcg tggcaagcac
     3481 cgtcaggata ccgataacat accgcatgac atcctccttg ttttccgaca ccgagcgata
     3541 ttggcacaac gcgccggtaa agtcaatagc gccttaccgc gcgacatacc gtaccgggtc
     3601 gacgatcccc gcttcggcga accccttgag tcgcaggcgg catgagtcgc aggtcccgca
     3661 ggcggcaccg tcggatgcgg ggtcgtagca actgtgggtg agcgagtggt cgacccccag
     3721 ccgcacgccc cactcgacga tctccttttt tgagagggcg atgagcggcg cctcgaccgc
     3781 aagacggtcg cccgcctttg tgccggaccg gaccgcccgt tcgaacgctt cgatgaaggc
     3841 ggggcgacag tcggggtacc cggagaaatc gaccgcgttg accccgatga tgatgcgttc
     3901 cgcaccgata tcctcggcgt aggccgccgc gagggaaaga aagatgagat tgcgggccgg
     3961 cacataggtg ataggtatcg cgtccgcgtc gtcggtatgg cgcggcaccg cgagcgagcg
     4021 gtcggtcagc gcggaggagt gaaggaagga gagatcgaac gatatctcgc gggcgcggtc
     4081 ggcgaggcgg taacggtcga gcgtgcggcg cgccttctcg atctcgaccc ggtgtcgctg
     4141 gccgtaactt atcgtcagcg ggtgcacttc gtcgcctcgg gcgatggcta tggcgagcgc
     4201 ggtggtcgag tcgagtccgc cgctgaagag cacgacgctt ttcacgggcg tttcctccgt
     4261 tccagttttc ccgcgatcgc ctcatacatg gcgagcatgg cgccggtcgc ggcgagcgga
     4321 agtgccgcac gcgccgcgag aggaagtacc ccggctatcg cgcagaaaag gactatgagc
     4381 gcgacggtgt ggatgctgaa atgtctgcgg atcgatcgtt cgatgcgccg caccggcccg
     4441 cctggagcct cggcgcggtg gcgatagcgg tcgtaacgga tgacgagcag ggggtaaaga
     4501 aaagagaaga cgatgccggg gaaataggcc cacggtatgt cgcgggagag gagggtgatg
     4561 gtgaggaaat aagcccacac tacaagacgg ctcgaataat agttcagtat gccgccctgc
     4621 cgcgaggtgc ggaaggtgag ccgcgccgtc tcgccgtcga ccgccgagac gatcgcggcg
     4681 gcgcagagca ggaacggggg aactatacct tcgagtgacc cgttaaggag ggggagcaac
     4741 gcagcggtga tgccgagaag catcgcgagg agcgtccaga ggttcgggct cacgcggtaa
     4801 ttggcggtcc acgaggtcag aaaacggctt atcgggcggg taaggttccg ttcgacgatg
     4861 tcatcgccct tgtggacgag cgaccggagc aggtattgtt cggccgccgc gagatcggcg
     4921 acagcacgca cggcgaagta gcgggccgct atgaccgcca cgcgggtgtc ctgccgccgg
     4981 tcgacgaatt cgcgcagacg cggccacgga tcgtcgaaga aatcgtcgct caggtcggcg
     5041 gcgcggagcc gggcgaagcc gataggttcg agcgtcgcag ggtcgcaggc gacgaccggc
     5101 gggtcgaagt tgaccgtcag gttgcgaagg aacggtccgt cgaagagaag gagcgggtcg
     5161 gcgaggatga gcgtatcagt taccgcgggg agagagggct cgatcgcgat gccgcgaaat
     5221 cgggagggag aggggagatg cggag
//
LOCUS       DAOQUE010000168         5218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_17507_length_5218_cov_4.056169, whole genome shotgun sequence.
ACCESSION   DAOQUE010000168 DAOQUE010000000
VERSION     DAOQUE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17507_length_5218_cov_4.056169"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PKH10_06350"
     CDS             <1..635
                     /locus_tag="PKH10_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27780.1"
                     /translation="PRTKITGWIKDLRDETTYPIDAHGYRENSWGRWAFNQGGWDFGT
                     HSDVRSKVMFGWQTYHFKSTRLDYLDLGFIDNGEYKLINFRADMGQLGWKHDLWSFDP
                     VARQCVPRDTTLIAKNADYRVDAYITIGQGQVPMLSSATSTVEKYVIMIQIPWFKGTI
                     TRLSDNTVIATFEGRGGGEFSTERAAEGTTNITDADCAAWGAKFSVPFPQ"
     gene            674..1996
                     /locus_tag="PKH10_06355"
     CDS             674..1996
                     /locus_tag="PKH10_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27781.1"
                     /translation="MKRALFGVFGAMAALALLWSCESGVKTPGNDQTTVADQDIAGGD
                     TVLVDIDDAEQEDFDRPCTGDETACSGDIYLWCNNGDFSEIDCAEFNKVCDPALGCVT
                     PTQDDTPPTDTDVVVDTDGDTIVIPDDDGEMPEGDDAGPSDEDLFPLDTADDPTVPDD
                     APIVVEDDAVVPDTDTDIVTDTPVPDVDVDADVDTDAPPPACTLSSTWPNTKPSEWTS
                     YLTLRLSGIINDADDADPDYAFLAAVSARLKGVTHNLSDNNGMFFRDLLDGSTPVVVE
                     SSMGGLTWPVSGQLASIWVSQTFVVVDDLLAWKETATAEGLTGVEVEGVYQVAVFEVW
                     IEIIGSSSQATRMECTRGISALNEAEDAYDGAMFFCVDTNTSWAIGETMKMMEYARML
                     DQDADILAALNEGLDPSDPDYLTDVCTCYEQDGVTPMDCDDMKAEFGL"
     gene            2111..3910
                     /gene="lepA"
                     /locus_tag="PKH10_06360"
     CDS             2111..3910
                     /gene="lepA"
                     /locus_tag="PKH10_06360"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNT27782.1"
                     /translation="MTKNIRNFSIIAHIDHGKSTLADRLMEMTGALSAREKRDQFLDD
                     MELEKERGITIKARAVTLPYKFEGETYELNLIDTPGHVDFAYEVSRALSSCEGALLVV
                     DATQGVEAQTLANVYMALDHDLEIVPVINKIDLQSAEIERARHEIEDIIGIDTSDAAL
                     VSAKTGQGVPELLEKLIKRVPPPREQAPGETRALIFDSWFDPYLGVRLLIRVFDGEFK
                     MGDKIWLMYSEKEYEILEIAREMPFMTKVPVLGTGQVGIIAAGIKNIKEVRIGDTVTL
                     AKRRAPEPLKGFKHMKQMVFCGIFPVESSRYEELKTAMEKLALNDSSFSYEQENSSAL
                     GFGFRCGFLGMLHLDIVKERLEREFDLELITTAPSVSYKVITAKGEELYIQNPDKLPP
                     VQHIDHIEEPFVKIAIHTPAEYIGPLVKLCQDKRGRQTNLHYLTPDRVMIEYAIPLAE
                     IVYDFYDKLKSISRGFASMDYEFLDYEVSDLVKLDILINGDPVDALSVIVFKDSAYQM
                     GRSLTQKMRKVIPRQMYEVAIQAAIGSRVLARESVKAFRKDVTAKCYGGDVTRKRKLL
                     EKQKEGKKRMKQVGMIEIPQEAFLAVLNAQETE"
     gene            3926..4825
                     /gene="lepB"
                     /locus_tag="PKH10_06365"
     CDS             3926..4825
                     /gene="lepB"
                     /locus_tag="PKH10_06365"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNT27783.1"
                     /translation="MLFKILVALTAMYFVLDAILARRGRRVFPFFKPKTVERLIERRR
                     QIEWILKCRDIPEEKRRIAQELVSSATTAIDDFKKKGHADLMAIAAQVDVAFAKHLRP
                     FKRNGFMEFVDSVWVVVLIALCIRFFLFESFRVPTGSMVPNIYIGDMLFVNKYIYGLR
                     PPFTKLHFFRFKEPTRGEVVIFMYPENPSQDFVKRVIGLPGDRIELRGKRVVVNGVEV
                     RRERLGLFNYVESRGGEREAEWFIETNQDGVSYQVLYSQGADIDHSGFLFDCKFCDSE
                     FTVPDDALFVMGDNRDVSLDSRF"
     gene            4951..>5218
                     /locus_tag="PKH10_06370"
     CDS             4951..>5218
                     /locus_tag="PKH10_06370"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNT27784.1"
                     /translation="MLAYAVTGAQWGDEGKGKIVDVLAEKMEAVVRFQGGNNAGHTIV
                     IDGRKHVLHTIPSGIFHAGVLNFIGNGVVLDPEEFLAEVAGLAAG"
BASE COUNT         1150 a         1546 c         1566 g          956 t
ORIGIN      
        1 ggccacgcac caagataacg ggctggatca aggatttgcg cgacgagacc acctatccga
       61 tagatgctca cggctaccgc gagaattcgt gggggcgctg ggccttcaat cagggtggct
      121 gggatttcgg cacccactcc gatgtgcggt cgaaggtgat gttcggttgg cagacctacc
      181 atttcaaatc gacgcggctc gactatctcg atctcggctt catcgacaac ggcgagtaca
      241 aactgatcaa tttccgcgcc gatatgggac aactcggctg gaagcacgat ctctggtcgt
      301 tcgacccggt cgcccggcag tgcgtcccgc gcgacacgac gctcatcgcc aagaacgccg
      361 actatcgcgt cgatgcctac atcaccatcg gtcaagggca ggtgccgatg ctttcttcgg
      421 cgacatccac cgtcgagaag tatgtcatca tgatacagat accgtggttc aaagggacca
      481 tcacgcggtt gtctgacaac accgtcatcg ccaccttcga ggggcgcggc ggcggcgaat
      541 tctcgaccga acgggcggca gaggggacca cgaacatcac cgacgccgac tgcgccgcgt
      601 ggggcgcgaa gttctcggta ccgttcccgc agtaggatat cggagagtaa caaccgagta
      661 ggggggatgc gtcatgaaaa gagcattgtt cggggtcttc ggcgcaatgg cggcgctggc
      721 cttactctgg tcctgcgaga gcggcgtgaa gacaccgggt aacgaccaga cgacggtcgc
      781 cgatcaggac atcgccggag gcgacacggt gcttgtcgat atcgacgatg cggagcagga
      841 ggatttcgac cgtccctgca ccggcgacga gaccgcctgc agcggcgaca tctacctctg
      901 gtgcaacaat ggcgatttca gcgagataga ctgtgcggaa ttcaacaagg tctgcgatcc
      961 ggcgctcggc tgtgtcacgc cgacgcagga cgatacgccg ccgaccgaca ccgatgtggt
     1021 cgtggatacc gacggtgaca ccatcgttat ccccgacgat gacggagaaa tgccggaagg
     1081 cgacgatgcg ggaccttccg acgaggatct gttcccgctc gataccgccg acgatccgac
     1141 cgtgcccgac gacgctccga tcgtcgttga ggacgatgcg gtcgtccccg acaccgatac
     1201 cgatatcgtc accgacaccc cggtacccga tgtcgatgtc gacgccgatg tcgataccga
     1261 tgcgccgccg ccggcctgca cgctgagcag cacgtggccg aacaccaaac ccagcgagtg
     1321 gacctcctat ctcacgctcc ggctgagcgg gatcatcaac gatgccgatg atgccgaccc
     1381 cgactacgcg ttccttgcgg cggtcagcgc acgactcaag ggagtcactc acaatctgag
     1441 cgataataat ggtatgttct tccgcgacct gttggatggt agcaccccgg tggtggtcga
     1501 aagcagtatg gggggactca cctggccggt gtcggggcaa ctcgcctcga tctgggtcag
     1561 ccagaccttt gttgtcgttg acgacctgtt ggcatggaaa gaaaccgcca cagccgaggg
     1621 gctgaccggc gtcgaggtcg aaggggtcta tcaggtcgcc gtcttcgaag tgtggataga
     1681 gatcatcggt tcgagcagtc aggcgacccg catggaatgc actcgcggga tctcggcgct
     1741 taacgaggcg gaggacgcct acgacggggc gatgttcttc tgcgtcgaca ccaacacctc
     1801 gtgggcgata ggggagacga tgaagatgat ggagtatgcc cgcatgctcg atcaggacgc
     1861 cgacatactc gcggcgctca acgaggggct cgacccgagc gaccccgact atctcaccga
     1921 tgtctgcacc tgctacgagc aggacggcgt caccccgatg gactgcgacg acatgaaggc
     1981 cgaattcgga ctgtagttat accccaatca ttccgcgcgc gaaccccaaa taattgttgc
     2041 ttttcgtccg ccccatgcta tagtcggccc ggtgtaacgg aacggggata gatacccggg
     2101 ctttgacaac atgaccaaaa acattcgtaa cttctcgata atcgcccaca tcgaccacgg
     2161 caaatccaca cttgccgacc gcctcatgga aatgaccggc gccctttcgg cccgtgaaaa
     2221 gcgcgaccag ttcctcgacg acatggaact ggaaaaagag cgcggcatca ccatcaaggc
     2281 gcgcgccgtc accctgccgt acaaattcga gggcgagacc tacgaactca atctcatcga
     2341 cacccccggc cacgtggact tcgcctacga ggtcagccgc gcgctctcat cctgcgaagg
     2401 agcgctcctc gtggtcgacg ccacgcaggg ggtcgaagcg caaacgctcg ccaatgtcta
     2461 catggcgctc gatcacgacc ttgagatcgt gccggtcatc aacaagatag acctccagag
     2521 cgccgagata gaacgggcgc gccatgagat agaggacatc atcgggatag acaccagcga
     2581 cgcagcgctt gtttcagcca agacggggca gggggtgccc gaactgctgg agaaactcat
     2641 caaacgggtg ccgccacccc gggaacaggc accgggcgaa acgcgcgcgc ttatcttcga
     2701 ctcgtggttc gacccgtatc tcggcgtccg gcttctcatc cgcgtgttcg acggtgagtt
     2761 caagatgggc gacaagatat ggctcatgta ttccgagaag gagtacgaga tactcgagat
     2821 agcccgcgag atgcccttca tgaccaaggt gccggtcctc ggcacggggc aggtcggcat
     2881 catcgccgcc ggcataaaga acatcaagga ggtccgcatc ggcgacacgg tgacgctggc
     2941 gaaaagacgc gcgccggaac cgctcaaggg cttcaagcac atgaagcaga tggtcttctg
     3001 tggcatattc ccggtcgagt cctcccgcta cgaggagctc aagaccgcga tggagaaact
     3061 ggcgctcaac gacagttcct tctcctacga gcaggagaac tcctccgcgc tcgggttcgg
     3121 gttccggtgc gggttcctcg ggatgcttca cctcgacatc gtgaaagagc ggctggaacg
     3181 cgagttcgac cttgaactga tcacgaccgc cccgtcggtc tcctataagg tgatcacggc
     3241 gaagggggag gaactctaca tccagaatcc cgacaaactg ccaccggtcc agcatatcga
     3301 ccacatcgag gaaccattcg tcaagatagc gatacatact ccggccgaat acatcgggcc
     3361 gctcgtgaaa ctctgtcagg acaagcgggg gcgccagacc aacctgcatt acctcacccc
     3421 cgaccgggtg atgatagaat acgcgatacc gctcgccgag atcgtgtatg atttctatga
     3481 caaactgaaa agtatcagcc gcggcttcgc ttcgatggac tacgaattcc tcgactacga
     3541 ggtctccgat ctcgtcaaac tcgacatcct catcaacgga gacccggtcg acgcgctgtc
     3601 ggtcatcgtg ttcaaggatt cggcctacca gatggggcgg tcactcaccc agaagatgcg
     3661 taaggtcatc ccgcgccaga tgtacgaagt ggcgatacag gcggcgatcg ggagccgggt
     3721 gctcgcccgc gaatcggtca aggcgttccg caaggatgtc accgcgaaat gctacggtgg
     3781 cgatgtcacc cgcaaacgca aactgctcga aaaacagaaa gaaggcaaga aacggatgaa
     3841 acaggtcggc atgatagaga taccgcagga ggcgttcctc gcggtcctca acgcgcagga
     3901 aactgagtag gggagaaacg gaaagatgct attcaagata ctcgtcgcgc tcactgccat
     3961 gtacttcgtc ctcgacgcga tacttgcccg ccgcggccgc cgcgtgttcc cgttcttcaa
     4021 gccgaaaacg gtcgagcgcc tcatcgagcg ccgtcgccag atcgaatgga tactcaaatg
     4081 tcgcgatatc ccagaggaaa agcgacggat agcgcaggaa ctcgtctcct cggcgaccac
     4141 ggccatagat gacttcaaaa agaaaggtca cgccgatctg atggcgatcg cggctcaggt
     4201 ggacgtggca ttcgccaagc acctgcgtcc cttcaagcgg aacgggttca tggagttcgt
     4261 cgattcggtc tgggtggtgg tgctcatcgc cctctgcata cggttcttcc tgttcgaatc
     4321 gttccgcgtc ccgaccgggt cgatggtgcc caacatctac atcggcgaca tgctcttcgt
     4381 gaacaagtac atctacggcc tgcgcccgcc gttcaccaaa ctgcacttct tccgtttcaa
     4441 ggaaccgacg cgcggcgagg tggtgatatt catgtacccc gagaacccct cgcaggattt
     4501 cgtgaaacgg gtcatcgggc ttcccggcga tcggatagaa cttcgcggga agcgggtcgt
     4561 cgtgaacggg gtcgaggtcc ggcgcgagcg gctcggcttg ttcaattatg tcgagagccg
     4621 cggcggcgaa cgcgaggccg aatggttcat cgagaccaat caggacgggg tctcctatca
     4681 ggtcctctac agtcagggag ccgatatcga tcactccggg ttcctgttcg actgcaaatt
     4741 ctgcgattcg gaattcacgg tgcccgacga tgctctcttc gtgatgggcg acaatcgcga
     4801 tgtgtccctc gattcgaggt tctagggctt cgtcccgatg gagaacctca agggacgcgc
     4861 ctcgatgata tggttctcgg tcagtatgca ggaagggttc caccttgacc ggatgggaga
     4921 gttcataaaa tgagaacggg agataacggt atgctcgcct atgcggtcac cggtgcccag
     4981 tggggcgacg agggaaaggg gaagatcgtc gatgtgctcg ccgagaagat ggaggcggtc
     5041 gtccgttttc agggcggcaa caacgccggc cacaccatcg tcatcgacgg cagaaagcat
     5101 gtgctccaca ccataccgtc ggggatattc cacgcgggag tgctcaactt catcggtaac
     5161 ggcgtggtgc tcgatccgga ggaattcctt gccgaggtcg cggggctcgc ggcgggtg
//
LOCUS       DAOQUE010000169         5200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_17601_length_5200_cov_3.610884, whole genome shotgun sequence.
ACCESSION   DAOQUE010000169 DAOQUE010000000
VERSION     DAOQUE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17601_length_5200_cov_3.610884"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PKH10_06375"
     CDS             <1..450
                     /locus_tag="PKH10_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27785.1"
                     /translation="KIFCRVDLGMQYTTGGNAYNYITDARWIYDDIDPETLYGWVLPA
                     GKDKYYHLLPLEDPFFSTTFLFKMNFRIFEYFQFGGHASVGYRHKHYLTAAKGDALIR
                     EPGFIPGLDSEETPWLGTTVVAQRSGGRILSEANVIVGWGFYLSGMF"
     gene            456..1874
                     /locus_tag="PKH10_06380"
     CDS             456..1874
                     /locus_tag="PKH10_06380"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNT27786.1"
                     /translation="MLSGKTILFVRVCGTGVSSAALFAQRMGARVLGTDSAYYPPISD
                     SLRNAGIAENPLADTIAIIEREKPDIIVVGNALWGKSPEAAYTLSCGIPYYSMPSFLE
                     EFLIPGHRSVVVAGTHGKTTTTTLIAEFLATVGKKPSYMIGGIPLFSMTNAAFEPSGH
                     FVIEGDEYDTAFFDKGPKFLHYRPDIGIITSIEYDHADIYASLEAIFESFRKFTAIIT
                     KRCILNIDFPNNKKLLTLFPRELFFTYSVTDPSADLFARRTGSEGRLMKFRVTVNGRE
                     GEEFKSPLFGDQNLMNLAALMAFIHNELSALDHATVAGAIAGMKGVKRRQEFLGHLNG
                     APVYSDFAHHPTAVRYTFDSFRSLFGHKRYIVVFDPATNSNARNIFEAEYAKLFADAD
                     LFFIGKPAKATGLSEEERFNPERLCDAINRVKGYPAARFMPEADQIVEALRAEADKES
                     VVIAMSNGGFGGFFGKLAPYLS"
     gene            2067..2978
                     /locus_tag="PKH10_06385"
     CDS             2067..2978
                     /locus_tag="PKH10_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27787.1"
                     /translation="MRKVLILLALVVVSLALSAEEKKAKLAVFDTTDKSKKFSKERLI
                     ASAEFIRGEIIASNQFIVIPKERQDKILIKEQKKESYKECYDQKCQIPLGQALSADTI
                     VVPTITLFDGIYTLRLEMIDLAKEATVNGATVDFTDQKNFRDSARKAVQKLLGLEVTE
                     TVKEEVRPKEEETRPVREDNRYPGENFDASKFSEPILDDWVFFFQDKKGKYYYNRRTI
                     LIHERRSLLLDPKERIEEVSAWVRIVNSSKVKEEKRYSIACESREVHPDGYGYASHDI
                     PITPESFLDYLHSKICSEEKQERGDFE"
     gene            2975..3427
                     /locus_tag="PKH10_06390"
     CDS             2975..3427
                     /locus_tag="PKH10_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27788.1"
                     /translation="MTITLSGDLSSLAAWYAAIIATLALLVQIVQHIINERVRVKVMY
                     GVNYVLSTNPSQKLVRIDILNIGRRPVTISAVSAFPKERTDKIIVLPESLDPLELLEG
                     KSKSYYFPQEDGLLDKVDCFIVNDMIGKSFKVKYRGDHRDQKVRGMHS"
     gene            complement(3510..4532)
                     /locus_tag="PKH10_06395"
     CDS             complement(3510..4532)
                     /locus_tag="PKH10_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNT27789.1"
                     /translation="MSRAAGTVAFTTTIPVEVILAAGRRPLDLNNIFISDPRAAELVE
                     KAEAAGFPRSCCAWIKGLFSVLRDRHRELGITAFVAVTEGDCSNAKVLKELIEREVGL
                     PSTFFSFPQDRSVAALKSEMEKLAAFLGTTPADAEKSRQALRPIRRSLEELDELSWRY
                     PGLVTGADNHRWLVSASDFNGDPAAFEKELHALLADKKAKTPEKRRDLLPLAYLGVPP
                     IVDLYPFIEEKGAIVVFNEMQREFSMPGEFPGLAEQYAAYTYPYAAEYRFSKAINEIR
                     RRGVKGVIHYVQSFCHRQLEDIILRRMLEEAGMKIPVLTLEGDKPQRVLDGRTRTRLE
                     AFIEML"
     gene            complement(4529..>5200)
                     /locus_tag="PKH10_06400"
     CDS             complement(4529..>5200)
                     /locus_tag="PKH10_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNT27790.1"
                     /translation="NQANWKTFEAKPFVGGTNTAHDPDGVGWIKREQWDAAKWDGDDS
                     WIRDIAATVVAIGEVGMDLHYDFSPPDRQEKVFRRMVELAIELRKPLVIHGRKAEGRI
                     FDMLSEYEEMDDRVLFHCYTGDVATARRIFDRNWSISLPGILTFPDAEEMREVLMMAP
                     AGQICFETDSPYLAPVPHRGKVNTPAHVTDIYRFAAQLTGLPLPVWEERAQQCFERLF
                     GGVTG"
BASE COUNT         1156 a         1461 c         1450 g         1133 t
ORIGIN      
        1 aagatattct gccgggtcga cctcgggatg cagtacacca ccggcggcaa cgcctacaac
       61 tacatcactg acgcccgctg gatatacgat gacatcgacc ccgagacgct ttacggctgg
      121 gtcctgccgg ccggcaagga caaatattac cacctcctgc cgctcgagga tcccttcttc
      181 tcgacgacct tcctcttcaa gatgaatttc cggatattcg aatatttcca gttcggcggt
      241 cacgcctcgg tcggttaccg tcataaacat tacctgaccg ccgcgaaagg cgacgccctc
      301 atccgcgagc ccggatttat tcccggcctc gattcagaag agacgccttg gctcggcacc
      361 acggtggtcg ctcagcgctc tggcggacgg atactgagcg aggccaatgt cattgtcggc
      421 tgggggttct atctctccgg catgttctga ccgatatgct ttccggtaaa acgatcctat
      481 tcgtacgtgt atgcggcacc ggcgtcagtt cggcggcgct ctttgcccaa cggatgggag
      541 cccgcgtcct cggtaccgat tcggcctact acccgcccat cagcgactcg ctcaggaacg
      601 ccgggatcgc cgagaacccg ctcgccgata ccatcgcgat catcgagcgg gaaaagccgg
      661 acatcatcgt ggtcggcaac gcgttgtggg gaaaaagccc cgaagcggca tacaccctct
      721 cgtgcggcat accgtactat tcgatgccgt cattcctcga agaattcctg ataccgggac
      781 accgctcggt cgtcgtcgcc ggtacccacg ggaagaccac caccaccacg ctcatcgccg
      841 aattccttgc caccgtgggc aagaagccgt cgtacatgat cggcggcata ccgctcttct
      901 cgatgaccaa cgccgctttc gagccgtcgg gccatttcgt gatagagggc gacgaatacg
      961 acaccgcctt tttcgacaag gggcccaagt tcctccatta ccgtcccgac atcggcatca
     1021 tcacctccat cgaatacgac cacgccgaca tctacgcgtc gctcgaggcg atattcgaaa
     1081 gtttccggaa gttcaccgcc atcataacga aacggtgcat cctcaatatc gatttcccga
     1141 acaacaagaa actgctgacc ctcttcccgc gcgaactttt cttcacctac tcggtgaccg
     1201 acccgtcggc cgacctgttc gcccgccgca ccggttccga ggggcgtctg atgaaattcc
     1261 gcgtgacggt gaacggcagg gaaggggagg agttcaagag tccgcttttc ggcgaccaga
     1321 acctcatgaa tctcgcggcg ctcatggcct ttatccacaa cgaactgtcc gcccttgacc
     1381 atgcgacggt cgccggtgcc atcgccggga tgaaaggggt gaagcggcgg caggaattcc
     1441 tcggccacct caacggtgcg ccggtctaca gcgatttcgc ccaccatccg acggcggtcc
     1501 gctatacctt cgattcgttc cgcagtcttt tcggtcacaa acgatatatc gtcgtgttcg
     1561 atccggcgac caattcgaac gcccgcaaca tcttcgaggc cgaatatgcg aaactcttcg
     1621 ccgacgccga ccttttcttc atcgggaaac cggccaaggc gacgggactc agcgaggagg
     1681 agcggttcaa tcccgaacgg ctctgcgacg ccatcaaccg cgtgaagggc tatccggcgg
     1741 cgcggttcat gcccgaggcc gaccagatag tcgaggcgct gagggcagag gccgataagg
     1801 agagcgtcgt catcgcgatg tccaacggcg ggttcggcgg ctttttcggg aagttggcac
     1861 cgtatctgtc gtagagacgc cccgatctct tcctcgccct gtgaagggag aggatcgagg
     1921 tgagggtgtt ctgaaaaccc tcatccggcc tgacggccac cttctccctc gataggtaga
     1981 aggttctggc cgccgcatgt tttcttgata tcttatttcg cctacgctac aatcggcgtg
     2041 ttttagggtc ggttagggga gttcttatgc gaaaggttct gattcttctt gcgttggtgg
     2101 tcgtcagtct cgcactgtct gccgaagaga aaaaggcgaa acttgccgtc ttcgatacaa
     2161 ccgacaaatc aaagaagttc agcaaggaac ggctgatcgc gtcggccgag ttcatccgtg
     2221 gagagataat cgcatccaat cagttcatcg tcatcccgaa agagcggcaa gacaagatac
     2281 ttataaagga acagaaaaaa gaatcgtaca aggaatgcta cgaccaaaag tgtcagatac
     2341 cgctcggtca ggcgctgtcg gccgacacga tagtcgttcc gacgataacg ctgttcgacg
     2401 gcatctacac gctccggctt gagatgatag acctcgccaa agaggcaacg gtgaatggcg
     2461 ccaccgtcga cttcaccgac cagaagaatt tccgggactc ggcgcgaaag gcggtacaaa
     2521 aactcctcgg actcgaagtg acggaaacgg taaaggaaga agtgcgtccg aaagaggaag
     2581 agacaagacc cgtcagagaa gacaaccgct atcccggcga gaacttcgac gccagcaaat
     2641 tctcagaacc catcctcgac gactgggtgt ttttcttcca ggacaagaaa gggaaatatt
     2701 attataatag gagaacaatc cttatacatg agcgccgctc tctattatta gatcctaaag
     2761 agagaataga agaggtcagt gcttgggtca gaattgtgaa ttcgagcaaa gtaaaagaag
     2821 aaaagagata ctctatagca tgcgagagta gagaggtgca tcccgatggg tatggctatg
     2881 cttctcatga catcccgata acaccagaga gttttttgga ttatctgcat tcaaaaatat
     2941 gctctgaaga aaaacaagag aggggagatt ttgaatgaca ataactctta gcggcgatct
     3001 ttcttctttg gcagcatggt atgctgcaat tatagctact ttggcgttgc tggttcaaat
     3061 cgttcaacac attatcaatg aacgcgtcag agtgaaggtg atgtatgggg ttaattatgt
     3121 tctctcaacg aacccctctc agaaacttgt ccgcattgac attctgaaca ttgggagaag
     3181 gccggtaact atttctgccg tgtctgcctt tcccaaagaa agaaccgata aaattatcgt
     3241 tttgcccgaa tcgcttgacc ctttagaatt gcttgaagga aaaagcaaat catattattt
     3301 cccacaggaa gacggccttc ttgataaggt agattgcttc atcgttaatg acatgatagg
     3361 aaagagtttc aaggttaagt accggggtga tcatcgtgac cagaaggtga gaggcatgca
     3421 ttcatagata ctgacggagg ctgggtcgca ctacagtttc ttgcctcttt tttaatgtat
     3481 catcctgtta attctgggtt cttatttttt tacagcatct cgataaaggc ctctagccgc
     3541 gtccgggtcc tgccgtcaag cactcgttgc ggcttgtcgc cctccagcgt cagcaccggt
     3601 atcttcattc ccgcctcctc cagcatccgt cggagtatga tatcctccag ttgccggtgg
     3661 cagaacgact gaacatagtg gatgacgccc ttaacgccgc gccgtcgtat ctcattgatg
     3721 gctttggaga agcggtactc ggcggcgtag gggtaggtgt aggcggcgta ctgttccgcg
     3781 aggccgggga attcgcccgg catcgagaat tcccgttgca tctcgttgaa gacgacgatc
     3841 gccccctttt cctcgataaa aggatagaga tcgacgatag ggggaacgcc gagataggca
     3901 agcggtagaa gatcgcgcct tttttcaggg gtctttgcct tcttgtccgc gagtagcgca
     3961 tgcagttcct tttcgaatgc ggcggggtcc ccgttgaaat cggatgcgct caccagccag
     4021 cggtgattgt cggcgccggt gacgagcccc ggataccgcc acgagagttc gtcgagttct
     4081 tccagcgacc tgcggatcgg ccggagcgcc tgtcgggact tttcggcatc ggcgggcgtc
     4141 gtgccgagga aggcggcgag tttttccatc tcgcttttga gcgccgccac ggatcggtcc
     4201 tgcgggaacg aaaagaaggt cgatgggagc ccgacctccc gttcgatgag ttccttgagc
     4261 accttcgcgt tggaacaatc gccctcggtc accgcgacaa aggcggtgat gccgagttca
     4321 cggtgacggt cgcgcaggac cgagaacaga ccctttatcc acgcacagca actgcgggga
     4381 aatccggcgg cctccgcctt ttcgacaagt tcggcggcgc ggggatccga tatgaagata
     4441 ttgttgagat cgagcgggcg gcgaccggcg gcgagtatca cctcgaccgg gatggtggtg
     4501 gtaaaggcga ccgttccggc ggcacggctc atccggtgac cccgccgaag agccgctcga
     4561 agcattgctg ggcccgttct tcccataccg gcaacggcag gccggtcagt tgggccgcga
     4621 accggtatat atcggtgaca tgggcggggg tgttgacctt gccccggtgg gggaccggcg
     4681 cgagataggg ggaatcggtc tcgaaacaga tctgacctgc gggggccatc atgagcacct
     4741 cgcgcatctc ctcggcgtcg gggaaggtca gtatgccggg gagactgatc gaccagttgc
     4801 ggtcgaatat acggcgcgcc gtcgcgacat cgccggtata acagtggaaa agaacgcgat
     4861 catccatttc ctcgtattca ctgagcatat cgaatatccg gccctccgcc ttgcgcccgt
     4921 ggatgaccag cggcttgcgc agttcgatgg ccagttcgac catccggcgg aatacctttt
     4981 cctgccggtc gggcggggag aagtcgtaat ggaggtccat gccgacctcg ccgatggcga
     5041 ctacggtagc ggcgatgtcc cgtatccacg agtcgtcgcc gtcccatttc gccgcgtccc
     5101 attgttctcg ctttatccaa cctacgccat cggggtcatg tgcagtgttc gtgccgccca
     5161 caaatggttt agcttcgaag gttttccagt ttgcctgatt
//
LOCUS       DAOQUE010000170         5165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_17757_length_5165_cov_3.715068, whole genome shotgun sequence.
ACCESSION   DAOQUE010000170 DAOQUE010000000
VERSION     DAOQUE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17757_length_5165_cov_3.715068"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="PKH10_06405"
     CDS             <1..1207
                     /locus_tag="PKH10_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNT27791.1"
                     /translation="GRKFHRMSLGGLHDEAEIRGHRRTYIGALPGKIIEGIKHCGTVN
                     PVFMLDEIDKIGKDFRGDPSSALLEVLDPEQNFSFTDNYLGLPYNLSEVFFIATANWE
                     ETIPAPLKDRMEIITLSGYTDLEKTEIAKRYLIPRQIDNNGLTDKDLKFADDAVQFLI
                     RRYTREAGVRELERKVGAICRKSITLKTSGKKIAHRVTPKLVEQYLKLPPYSEESFLR
                     DERIGVVTGLAWTMHGGTTLKIEVNWMEGTGQLILTGQLGDIMKESARIALSYIKSNR
                     EAFGLPADFSLDKKDIHIHFPAGAVPKDGPSAGITITTALLSLFLKKTVNPGVGMTGE
                     ISLTGKVLPIGGLREKLLAAKRYGLAEVILPTDNQALHKSIPAEIRESVAATFVSDYH
                     EVFSKLFVS"
     gene            1217..2128
                     /gene="thiL"
                     /locus_tag="PKH10_06410"
     CDS             1217..2128
                     /gene="thiL"
                     /locus_tag="PKH10_06410"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNT27792.1"
                     /translation="MLDNREWRFIRELMTLDDPKRPFIGDDCAVWNDHHLLVTTDHMA
                     EGIHFDLSFMPPDAVGWRLMAANASDILAMGGRPSHFTLNLAIPADRQETARGIIEGI
                     KRFAKAHGIICIGGDTTAANALFLGATMFAPLPTPPWRRSGARPGDRIFLAGYPGLSR
                     AGLLRLQRGEQGGEATRRFLYPDPFAQPPVTGAPGAAIDISDSLVSELTLLAEASGVS
                     MDIAAERIPAHPEVAAAARELQVPLDEILLSSGEEFFLIVCSPHAQPEMFEIGTVTER
                     RDHALFVTRDGRPLDTAALPVWDHFGR"
     gene            2052..2513
                     /locus_tag="PKH10_06415"
     CDS             2052..2513
                     /locus_tag="PKH10_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNT27793.1"
                     /translation="MPFSSPATAGRWILLLCLCGTISAAENFCGYTPKATVAILSEGK
                     KSYSFLAAVADRPELHRRGLMGCAALPPKQGLLFIFDDLRERFFWMHDTMIPLAILYI
                     GDDGRIVSIKEGKPGSDATIPSGHPVRYALEVNLKEGLAVPVGATVKISFD"
     gene            complement(2510..2893)
                     /locus_tag="PKH10_06420"
     CDS             complement(2510..2893)
                     /locus_tag="PKH10_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNT27794.1"
                     /translation="MGFLSSLWDTIKPKREIVPVELNPDNFQPSIQNSPVPVLVDFYS
                     NTCMPCRQMAKTVTKFATDFQGRVRVGAFNTANDTQGKILVQFKVRAVPTLVMFNKGK
                     PVETFVGLTGYLKLAEALEKIENGG"
     gene            complement(2906..4459)
                     /locus_tag="PKH10_06425"
     CDS             complement(2906..4459)
                     /locus_tag="PKH10_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT27795.1"
                     /translation="MRGGRPVDIAFTGGGVVRGGDFLPAFRMPAVSYDLYLSREVFVS
                     FGPDMTVAEKREIAAHAGGELVRPVARTEGFWLVSLPYPSIGLVDRIADMPGVRRVEP
                     DTYRPMAWRADDPDDPLFPHQWHLENRGQVGRTGEDAKVAAAWRYLYDAGLEPGRGIR
                     VGVIDDGFDLAHTDLAGAFLAGIDLFDGDDLPMIGQGDLHGTAVAGVLAARVGNGIGV
                     AGACGGCLVIPVRVSAELINGVTEIDAFNYLLDREVDIISNSWGPADHGGPMDMSEPL
                     KEIIAWAAVHGRGGRGVTILFAAGNGNEDISDPHSFDGYAANPWVVAVGAVNASGVRT
                     VYSDWGRDLDILAPSCDTDLEGYADPFDPYKIRDGIWTTDNTGFSGYAPGDHTATFCG
                     TSSAAPLAAGVAALLLSAAPSLTREELYGLLTGTADKVSPRDARYDGTGFSERYGFGR
                     IDALAAIEELCAQKACEGAPADTEEPIEPLGPEVDFVENLNTVSDEDLPLVTERPVTT
                     GGGCSFTAL"
     gene            complement(4578..>5165)
                     /locus_tag="PKH10_06430"
     CDS             complement(4578..>5165)
                     /locus_tag="PKH10_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27796.1"
                     /translation="DTSVLLKFGTFTYGTYRTDVRAADIDEVEVINSVGNMYYRAKLP
                     QGDLSEIDIRDLVWWKERKAAGASDADALCLADPTDADDDGNADGYAWTTDDYGPVRG
                     AKIKCARDGSNNIIQKYDTKEDFKQYLEDSYARFCPAYRLVRTLSKITGQGEGSQAKS
                     DPSNYKVTVIETTLDQLNAFISDHFGTAVFYANFI"
BASE COUNT         1014 a         1731 c         1509 g          911 t
ORIGIN      
        1 cggccgcaag ttccaccgca tgagtctcgg agggctccat gacgaggccg agatacgcgg
       61 ccaccggcgc acctacatcg gcgccctgcc cggcaagatc atcgagggga tcaagcactg
      121 cggcaccgtc aacccggtct tcatgctcga cgagatagac aagataggca aggatttccg
      181 cggcgacccc tcgtcggcgc tcctcgaggt gctcgatccg gaacagaact tctcgttcac
      241 cgacaactat ctcgggctcc cctacaatct ttccgaggtc ttcttcatcg ccaccgccaa
      301 ctgggaagag acgataccgg cgccgctcaa ggaccgcatg gagatcatca ctctgtcggg
      361 ctacaccgac ctcgagaaga ccgagatagc caaacgctac ctcatcccgc gtcagataga
      421 caacaacggg ctgaccgaca aggacctcaa gttcgccgac gacgccgtcc agttcctcat
      481 ccggcgttac acccgcgagg cgggggtccg cgaactggaa cgcaaggtcg gcgccatctg
      541 ccgcaagagc atcacgctca agacctcggg caagaagata gcgcaccgcg ttaccccgaa
      601 actggtcgaa caatacctca aactaccccc ctattccgaa gaatcgttcc tccgcgacga
      661 gcgtatcggc gtggtcaccg gcctcgcatg gacgatgcac ggcggcacga cgctcaagat
      721 agaggtcaac tggatggagg ggaccgggca actgatactg actggacaac taggtgacat
      781 catgaaggaa tctgcccgca tagccctttc ctacatcaag agcaaccgcg aggcgttcgg
      841 gcttcctgcc gacttttcgc tcgacaagaa ggacatccac atccacttcc cggcgggggc
      901 ggtgcccaag gatggcccgt cagccggcat cacgataacg acggcgctcc tgtcgctctt
      961 cctcaaaaag acggtcaatc ccggtgtcgg gatgaccggc gagataagtc tcaccggcaa
     1021 ggtgctcccg atcggcggtc tgcgcgaaaa actgctcgcg gccaagcgct acggacttgc
     1081 tgaggtcatc ctgccgaccg acaatcaggc cctccacaag agcatacccg ccgagatacg
     1141 cgagagcgtc gcggcgacct tcgtatccga ctaccacgaa gtcttctcga aactgttcgt
     1201 ctcctaggcg cccgtcatgc tcgacaaccg cgagtggcgc tttatccgcg aactcatgac
     1261 gctcgatgac ccgaagcgcc ccttcatcgg cgacgactgt gccgtctgga acgaccatca
     1321 cctcctcgtc acaaccgacc atatggccga agggatacat ttcgatctct ccttcatgcc
     1381 gcccgacgcg gtcggctggc ggctcatggc ggctaacgcg agcgacatcc tcgcgatggg
     1441 cgggcgacct tcgcatttca cgctcaacct cgccatcccg gccgaccggc aggagacggc
     1501 gcgcggcatc atcgaaggca tcaaacgttt cgccaaggca cacggcatca tctgcatcgg
     1561 cggcgacacg accgccgcga atgcgctgtt cctcggcgcc acgatgttcg cccccctgcc
     1621 caccccgcca tggcgccgct ccggcgcacg cccgggtgac cgcatcttcc tcgcgggata
     1681 cccggggctc tcgcgggccg gactgctccg gcttcaacgc ggagaacaag gcggagaagc
     1741 aacgcgccgt ttcctctacc ccgacccgtt cgctcaaccg ccggtcaccg gcgcacctgg
     1801 cgcggcgata gacatcagcg actcgctcgt ttcagaactc accctcctcg ccgaggcgag
     1861 cggcgtttcg atggatatag cggccgaacg gataccggcg caccccgagg tcgccgccgc
     1921 cgctcgtgaa cttcaggtcc cgctcgacga gatcctgctt tcgagcggcg aggagttctt
     1981 ccttatcgtg tgctcacccc atgctcaacc ggagatgttc gagataggta ctgtcaccga
     2041 acggagggat catgcccttt tcgtcacccg cgacggcagg ccgctggata ctgctgctct
     2101 gcctgtgtgg gaccatttcg gccgctgaga acttctgcgg ctacaccccc aaggcgaccg
     2161 tcgccatcct gtcagaaggc aaaaagagct actcgttcct cgccgccgtc gccgaccggc
     2221 ccgaactgca ccgtaggggg ctcatgggct gcgccgcgct cccgccgaaa caggggctcc
     2281 tcttcatctt cgacgacctg cgggaacggt tcttctggat gcacgacacc atgataccgc
     2341 tcgcgatact ctacatcggc gatgacggtc gtatcgtatc gataaaagag ggaaagcccg
     2401 gttcggacgc cacgataccg agcggtcatc cggtccggta cgcgctcgag gtcaatctga
     2461 aggaagggct ggcggtgccc gtcggagcaa cggtgaagat atcgttcgac tagccgccgt
     2521 tctctatctt ctcaagcgct tcggccagtt tcaggtaacc ggtgaggccg acgaaggtct
     2581 cgaccggttt ccccttattg aacataacga gcgtcgggac cgcgcgcacc ttgaactgca
     2641 caagtatctt cccttgcgtg tcgttggcgg tattgaaagc gccgacccgc accctgccct
     2701 gaaaatcggt ggcgaacttg gtcaccgtct tggccatctg gcggcacggc atgcaggtgt
     2761 tcgaatagaa atcgacgagc accggcaccg gcgagttctg tatcgacggc tggaagttat
     2821 cggggttcag ttcgaccggc acgatctccc gtttcggctt gatcgtatcc cacagcgatg
     2881 aaagaaagcc catgagcgtc ctccgttaga gcgcggtgaa agagcaccca ccgccggtcg
     2941 tcaccggtcg ttcggtcacg agcggcaggt cttcgtcgct gactgtattc aggttctcta
     3001 cgaagtcaac ctccggcccc aacggctcga tcggctcttc ggtatcggcg ggcgcacctt
     3061 cgcaggcctt ttgcgcacaa agttcctcga tggcggcgag ggcgtcgatc cggccgaacc
     3121 cgtaccgttc gctgaagccg gtcccgtcgt accgcgcatc acgcgggctc accttgtcgg
     3181 cggtcccggt caaaagaccg tacagctcct cgcgcgtcag cgaaggtgcg gccgacagca
     3241 ggagcgccgc cacacccgcc gcgagcggtg cggcgctgga ggtgccgcag aaggtagcgg
     3301 tatgatcgcc cggcgcatag ccgctgaagc cggtgttgtc ggtcgtccag atgccgtcgc
     3361 gtatcttgta ggggtcgaac ggatcggcat acccttccag atcggtgtcg caactgggcg
     3421 cgagtatgtc gagatccctt ccccagtcgc tgtagacggt ccgcaccccc gaggcgttca
     3481 ccgcgccgac cgcgacgacc catgggttcg cggcatagcc gtcgaaacta tgagggtcgg
     3541 agatgtcctc gttgccgttg ccggcggcga acagtatcgt cacgccgcga ccgccccgtc
     3601 cgtgaacggc ggcccacgcg atgatctcct tgagcggttc gctcatgtcc atcggcccgc
     3661 cgtgatcggc ggggccccaa ctgttcgata tgatgtccac ctcccggtcg agcagatagt
     3721 tgaacgcatc tatctcggtg acgccgttta tcagctcggc actcacccgc accggaatga
     3781 cgaggcaccc gccgcaggcg cccgccacgc cgatgccgtt accgacccgc gccgcgagca
     3841 cgccggcgac cgccgtgcca tgcaggtctc cctgaccgat catcggcagg tcgtccccgt
     3901 cgaacaggtc gatgccggcg aggaacgccc cggcaaggtc ggtgtgggca aggtcgaaac
     3961 cgtcgtcgat gacacccacc cgtatcccgc gccccggctc aagcccggcg tcgtaaagat
     4021 accgccacgc cgccgcgacc ttggcgtctt cacccgtacg gccgacctga cccctgttct
     4081 cgagatgcca ctggtgcggg aagagcgggt cgtcggggtc atcggcacgc cacgccatcg
     4141 ggcgatacgt gtcgggttcg acccggcgaa cccccggcat atcggcgatg cggtcgacaa
     4201 gcccgatcga cggatacggc aagctgacca gccagaagcc ctcggtacgc gccaccggcc
     4261 tgacgagttc accacctgcg tgcgccgcga tctcgcgctt ttccgccacc gtcatatccg
     4321 gtccgaacga cacgaaaacc tcgcgggaaa gatagaggtc gtaggacact gcgggcatcc
     4381 ggaacgccgg taagaagtca ccgccccgca caacaccacc accggtaaag gcgatatcga
     4441 ccgggcgacc gccgcgcatc agcgtctcag cccgcaatat cgacgggaat atcaggaaaa
     4501 ggagcagaac gatatcggcc caaaaagaaa gggagggccc gaaggccccc cctgcaagaa
     4561 gcatagaact gtaagaccta gatgaaattc gcatagaaca ccgccgtccc gaaatgatcg
     4621 gagatgaagg cgttcaactg atcgagcgtc gtttcgatga ccgtcacctt gtagttcgac
     4681 gggtcggact tcgcctgact cccttcgccc tgaccggtga tcttgctgag cgttctcacc
     4741 agacgatagg cggggcagaa gcgggcatag gaatcctcaa ggtactgctt gaaatcctcc
     4801 ttggtgtcgt acttctggat gatgttgttg ctgccgtcac gcgcacactt gatcttggcg
     4861 ccacggaccg ggccgtaatc atcggtggtc cacgcatagc cgtcggcatt gccgtcatca
     4921 tcggcatcgg tcgggtcggc gaggcacagc gcgtcggcgt cggaggcgcc cgccgctttg
     4981 cgttctttcc accacacgag gtcgcggatg tcgatctcgc tcaggtcgcc ctgcggcagt
     5041 ttcgcgcggt aatacatgtt gccgaccgaa ttgatgacct cgacctcgtc gatatcagcc
     5101 gcccgcacat cggtacgata ggtgccgtag gtaaaggtac cgaacttcag caggacgctc
     5161 gtgtc
//
LOCUS       DAOQUE010000171         5095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18091_length_5095_cov_3.975794, whole genome shotgun sequence.
ACCESSION   DAOQUE010000171 DAOQUE010000000
VERSION     DAOQUE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18091_length_5095_cov_3.975794"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PKH10_06435"
     CDS             complement(<1..982)
                     /locus_tag="PKH10_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNT27797.1"
                     /translation="MRISFFVLLLCGVVIFFVVPLSAASPMPRFGNLFLSVVYDTDEE
                     EEGGGEEEDESSRDREEDDEDEEAPAKDEFTEKKPKDEFALEQRSEEVYEYKEPEIVV
                     AKRPTFLYLESSLDSRKIFEIFKGDELIVIEEQEGWVLVEFLGKQGWVERSDVSSTTY
                     AVYRLFCDLSVGLAYSPGLENYKMLGNYSVSVFYGLHEYFMFGGEFKALSVDSDVLYT
                     GGGGVIRGHIPYLESKRSRTVLSVSGGYFRMPETPMYKGAGSRPGIITFEGGYFSTAL
                     EHVVRVSDPVYIGGGFEFMYAAGEGKDGTGQTLHRDFWTVGGRIKVMFNIW"
     gene            complement(1072..3582)
                     /gene="gyrA"
                     /locus_tag="PKH10_06440"
     CDS             complement(1072..3582)
                     /gene="gyrA"
                     /locus_tag="PKH10_06440"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNT27798.1"
                     /translation="MVEQTNIVNVNEKIKPVSIEQQMKTSYIDYAMSVIVGRSLPDVR
                     DGLKPVHRRVLFAMKELGCTYTAPYKKSARIVGDVIGKYHPHGDQAVYQTVVRMAQDF
                     SMRYLLVDGQGNFGSIDGDEAAAMRYTEARMARIAGELLADIDKDTVTMLDNYDGSLK
                     EPEVLPARIPQLLINGTSGIAVGMASNIPPHNLTEVVKALIHFIEHPGASVADLMNFV
                     KGPDFPTAGLILGRGGIRQAYETGRGIVRMRSRATIEEGTKANDRPRIIIHELPYQVN
                     KAQTIIQIAELVKDKKIEGIHDIRDESDRRGIRVVIELKKDAIGDIVLNQLYKMTNLQ
                     TSFGINMLAICNGVPRTLNLRDVLNYFLMHRRDVVTRRTRFELDEAEKRAHILEGLKK
                     ALDVIDKVIAIIRKSPSTDEARESLMAKFGFSKVQASAILDMKLSRLTGLEREKIEAE
                     LAELHKKIKWYNEILANEKTLLSVIKNELKEIMERYGDERRTEIVDEAGDIDIEDLIP
                     NEEMVVTHTTENYIKRTPLAQYRKQKRGGKGINAINPKENDFVKDIFIASNHTLLMIF
                     TSYGKVYWVKVYQLPEAGRGSRGKPIINMVQVEKDERVMEILPIHEFVDGKFVFMATK
                     HGTVKKTDLMEFAIQRANGKKAINMLEGDELIGVAITSGDNEVILASRNGLSIRFPEK
                     QVRAMGRAATGVRGMLLEKADEVMAMEVVEPEKTLLSVTDRGIGKRTPFEDYRVQSRG
                     GKGIITIKTSDENGFVVGVLKVSGTDEAMLITSNGQAIRIPASGISVIGRNTKGVRLF
                     RVAEGEKVVSVTKIGEPSDNGAADDESDDEGGSEDAEK"
     gene            complement(3598..>5095)
                     /locus_tag="PKH10_06445"
     CDS             complement(3598..>5095)
                     /locus_tag="PKH10_06445"
                     /inference="COORDINATES: protein motif:HMM:NF036694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNT27799.1"
                     /translation="IREGLTAVLSVKMPDPKFSSQTKDKLVSSEIQTIVSNVVAEYLT
                     LYLDENPDIAGAIIDKILEAAIAREAARKAREMTRRKGALENTTLPGKLADCQERDPA
                     KSEIFIVEGDSAGGSAKQGRDRSNQAILPLRGKILNVEKARLDKLLKSEAVLTVVAAL
                     GTGIGKDEFNIEKLRYHKIIIMTDADVDGAHILTLLLTLFYRYMPDIIARGYLYVAQP
                     PLYGITRGKKVSYVRDEREFQEYLINTTGTEGVAVSFGKKKEGRLEEGKLRSFIKKLV
                     VLNDNLTRTEFLYDSAVVEAVASIGGIEEADFKTEKALSDKFEKVRAFIERDYPDRLP
                     VRMEFEENERYNELKLKIFTTHSGVEKQTLIDYRYIKNSEIILIGRLCGEVAAFGAAP
                     YTVTVSDKEKGTEEVRGVYPRWRDLLKATLDHAREGQKIQRYKGLGEMNPEQLWETTM
                     NPGNRTLLKVSADDAAEADRIFDILMGEIVEPRRRFIEENALNANIDV"
BASE COUNT          906 a         1520 c         1462 g         1207 t
ORIGIN      
        1 cccaaatgtt gaacatcact ttaatgcgac cgcccacggt ccagaagtca cggtgaagcg
       61 tctggccggt accgtccttg ccctctccgg ccgcgtacat gaattcgaag ccgccaccga
      121 tatagaccgg atcggagacg cggacgacat gctccagcgc cgtggagaaa tagccgccct
      181 cgaaggttat gatgccgggg cgtgaaccgg ctcccttgta catgggcgtt tcgggcatcc
      241 tgaaatagcc gccggaaacg gaaagcaccg tccgcgaccg tttgctctcc agatacggga
      301 tatggccccg gatgacaccg ccgccacccg tgtagagtac atcgctgtcg accgacagcg
      361 ccttgaactc accgccgaac atgaaatact cgtgaagacc atagaacacc gatacgctgt
      421 agttgcccag catcttgtaa ttctcgagtc cgggcgaata ggccagacca actgaaaggt
      481 cgcagaacag tcgatagacc gcgtaggtcg tggaggaaac atcgctccgt tcgacccagc
      541 cctgcttgcc gaggaactcg accagcaccc acccctcctg ttcctcgatc acgatcagtt
      601 cgtcaccttt gaatatctcg aatatctttc gggaatccag cgaactctcc aggtagagga
      661 aggtcggcct cttcgcgacg actatctccg gttccttgta ttcgtacacc tcttccgagc
      721 gttgttcgag cgcgaactcg tctttcggct tcttttcggt gaattcgtct tttgcggggg
      781 cttcctcatc ctcgtcatct tcctcccggt cacgggaact ttcgtcctcc tcttcgcccc
      841 caccttcctc ctcttcgtcg gtgtcgtaca cgaccgaaag gaaaaggttg ccgaaacggg
      901 gcattggcga tgcggccgac agcgggacca cgaaaaagat cacgaccccg cagagaagaa
      961 ggacaaaaaa agagatcctc acgacgactc ctgttatcgg cggtttttca gttcgctata
     1021 gtataggaaa agaaccgtaa aatcaagatt tggaagaagc ggggccggct tttatttctc
     1081 ggcatcctcg ctaccgccct cgtcgtccga ctcatcgtcg gcggcaccgt tatcagacgg
     1141 ttcccctatc ttggtgacgc tgacgacctt ttctccctcg gcgacgcgga acaggcgcac
     1201 ccccttggtg ttgcggccga tgacgctgat gccggaggcc gggatgcgta tcgcctgtcc
     1261 attgctcgtg atgagcatcg cctcgtcggt gccgctcacc ttcaacacac cgaccacgaa
     1321 cccgttctcg tccgaggtct ttatcgtgat gatacccttg ccgccgcgcg attgaacacg
     1381 ataatcctcg aacggcgtcc gcttaccgat gccgcggtcg gtgaccgaca ggagcgtctt
     1441 ttcgggctcg accacctcca tcgccatcac ctcatcggcc ttttcgagca gcatgccccg
     1501 cacaccggtc gcggcacgcc ccatggcgcg gacctgcttt tcggggaacc gtatcgagag
     1561 gccgttgcgc gacgcgagga tcacctcgtt gtcgccgctc gttatcgcca cgccgatgag
     1621 ttcgtcgcct tcgagcatgt tgatggcttt cttgccgttg gcgcgctgga tcgcgaactc
     1681 catgagatcg gtcttcttga ccgtgccgtg tttggtcgcc atgaacacga acttgccgtc
     1741 gacgaattca tggatgggaa gtatctccat gacccgctcg tccttttcga cctgcaccat
     1801 gttgatgatg ggcttgccgc gactgccgcg ccccgcctcc ggcaactggt agaccttgac
     1861 ccagtagacc ttgccgtaac tggtgaagat catgagaagc gtatggttgc tcgcgatgaa
     1921 gatatccttg acgaagtcgt tctccttggg atttatagcg ttgatccctt tacccccccg
     1981 cttctgtttg cggtactggg ccagcggcgt ccgtttgata tagttctcgg tggtatgcgt
     2041 gaccaccatc tcctcgttgg ggatgaggtc ctcgatgtcg atgtcgcccg cctcgtcaac
     2101 tatctcggtg cgccgctcgt cgccgtagcg ttccatgatc tccttgagtt cgttcttgat
     2161 gaccgacagg agcgtctttt cgttggcaag tatctcgttg taccacttta tcttcttgtg
     2221 tagttcggcg agttcggcct ctatcttttc gcgttccaac ccggtgagcc gcgacagttt
     2281 catgtcgagt atggccgacg cctgtacctt cgagaagccg aatttcgcca tcaacgattc
     2341 gcgcgcctcg tctgtcgaag gcgatttgcg gatgatcgct atcaccttgt ctatgacatc
     2401 aagcgctttt ttgaggccct cgaggatgtg ggcgcgtttc tcggcctcat ccagttcgaa
     2461 ccgggtgcgg cgggtcacca catcgcgccg gtgcatcagg aaataattca gcacatcgcg
     2521 caggttgagc gttcgcggca cgccgttgca aatggccagc atgttgatgc cgaaactggt
     2581 ctggagattc gtcatcttgt agagctggtt gagcacgatg tcgccgatcg cgtccttctt
     2641 tagttctatc accacgcgga tgccgcgccg gtctgattcg tcgcggatat catggatgcc
     2701 ctcgatcttt ttatccttga ccagttcggc gatctggatg atggtctgcg ccttgttgac
     2761 ctgatagggc aactcgtgga tgatgatgcg cggtcggtcg ttcgccttgg tcccctcctc
     2821 tatcgtggcg cgggagcgca tcctgacgat gccgcgtccc gtctcgtagg cctgccggat
     2881 gccaccgcga ccgaggatga gaccggcggt cgggaaatcg ggacctttga cgaagttcat
     2941 cagatcggcc acgctcgcac cgggatgctc gatgaaatgg atgagcgcct tgaccacctc
     3001 ggtaaggtta tgcggcggga tattcgacgc catgccgacg gcgatgccgg aggtgccgtt
     3061 gataagcagt tgcggtatcc gggccggcag gacctcgggc tccttgagcg agccgtcgta
     3121 gttatcgagc atcgtgaccg tgtccttgtc gatgtcggcc agcagttcac cggcgatacg
     3181 ggccatgcgc gcctcggtgt aacgcatcgc ggccgcctcg tcgccgtcga tcgaaccgaa
     3241 gttcccctgt ccgtcgacga gtaggtagcg catcgagaaa tcctgcgcca tgcggaccac
     3301 cgtctggtag accgcctgat cgccgtgcgg gtgatattta ccgatgacat cgccgacgat
     3361 gcgcgccgat ttcttgtacg gcgcggtgta ggtgcatccc agttccttca tcgcgaacag
     3421 cacccgccgg tggaccggct tgagcccgtc gcgcacatcg ggcagggacc gtcccacgat
     3481 gacgctcatc gcgtaatcga tgtaggaggt cttcatctgc tgttcgatgc tgaccggctt
     3541 tatcttctcg ttgacattga cgatattcgt ttgttctacc atggatgtct cccgacctta
     3601 cacatctatg ttcgcgttga gggcattctc ttcgatgaag cggcggcgcg gctcgacaat
     3661 ctcgcccatc agaatgtcga atatccggtc cgcctcggcc gcgtcatcgg ccgagacctt
     3721 gagcagggtg cggttccccg ggttcatggt cgtttcccat aactgttcgg gattcatctc
     3781 accgagacct ttgtagcgct gtatcttctg accttcgcgg gcgtgatcga gcgtcgcctt
     3841 gagcaggtcg cgccaacggg gatagacccc acgcacctct tcggtcccct tttccttgtc
     3901 cgaaacggtc accgtgtagg gggcggcgcc gaacgcggcg acctcgccgc acaggcggcc
     3961 gatgaggatt atctcggagt tctttatgta acggtagtcg atgagtgtct gtttctcgac
     4021 cccgctgtgg gtggtgaata tcttgagctt caattcgttg tagcgctcat tctcctcgaa
     4081 ttccatgcgg accggcaggc ggtcggggta atcgcgttcg atgaaggcgc gcaccttttc
     4141 gaacttgtcg gaaagcgcct tttcagtctt gaaatcggcc tcttctatgc cgccgatgga
     4201 ggcgaccgcc tcgaccaccg ccgaatcata aaggaattcg gtgcgggtca ggttgtcatt
     4261 gagcaccacc agttttttga tgaaggagcg gagtttcccc tcctccagac gcccttcctt
     4321 ctttttgccg aaggagaccg cgaccccctc ggtgccggtg gtattgatga gatattcctg
     4381 gaactcccgt tcgtcgcgga cataggagac cttcttgccg cgcgtgatgc cgtagagcgg
     4441 tggttgggcg acatagagat agccgcgcgc tatgatgtcc ggcatataac ggtagaagag
     4501 cgtcagaagg agggtcagga tgtgggcgcc atcgacatcg gcgtcggtca tgatgatgat
     4561 cttgtggtag cggagtttct cgatgttgaa ctcgtccttg ccgatgccgg tgccgagcgc
     4621 cgcgacgacg gtgagcaccg cctcgctctt gagcagtttg tcgagccgcg ccttttcgac
     4681 attgagtatc ttgccgcgca ggggcagtat cgcctgattg gagcggtcac gcccctgctt
     4741 ggccgagccg ccggccgaat cgccctcgac gatgaatatc tccgatttgg ccgggtcgcg
     4801 ttcctgacag tcggccagtt taccggggag cgtcgtgttc tcgagcgctc ccttgcgccg
     4861 cgtcatctcg cgcgctttgc gggccgcctc gcgggcgatg gccgcttcga gtatcttgtc
     4921 gatgatggcg ccggcgatgt cggggttctc gtcgagatag agggtcagat attcggccac
     4981 gacattggaa acgatggtct gtatctcgct ggagaccagt ttgtccttgg tctgggaact
     5041 gaatttcgga tcgggcatct tgaccgacag gaccgctgtg agcccttcgc ggata
//
LOCUS       DAOQUE010000172         5088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18127_length_5088_cov_4.331810, whole genome shotgun sequence.
ACCESSION   DAOQUE010000172 DAOQUE010000000
VERSION     DAOQUE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18127_length_5088_cov_4.331810"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PKH10_06450"
     CDS             <1..1070
                     /locus_tag="PKH10_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27800.1"
                     /translation="DLDPKILQNSYDYILTPYCWDTFSSEFPIYSLTGSTLSFYSSTA
                     SPYDGGDFIYDLDSRKLTRIGRGMGVSPFSNGEYFFSRTTDYSTVWEESYEEVVFYYH
                     FGNKTYGNAWKMPQKLSYVRNIVASDQYIAMNLVPLTGNRYEPLRGYYTKIGEWDKWV
                     ELNFGTYPSYGIGKPEIQGSYLVSYNYIDNLLRVFFCDLAKGESSCRIVSKAGEIARK
                     PIWGAKNTFYYSVEEENDGATVYRSTIGFVDGLGTKLISLPIFSSDKYMQVYQASEDF
                     LLYLKYIKEGSPFDIYDVCYYRFSDKKHFCLEDGINSDIEYQAPYFYDKWIVFQSKYD
                     LIIRDMDGYCQEHPENCPFEP"
     gene            1212..2435
                     /locus_tag="PKH10_06455"
     CDS             1212..2435
                     /locus_tag="PKH10_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNT27801.1"
                     /translation="MRPATSIVFMIALFATPFLYALEPVPAGATQDLIEKKEQAEEKK
                     AEDEKVEEKKPEEKKEKPRNDYIIIPAIYYTPETSVAFGLSVLFYYRPPNQLLERRPA
                     VIQPTVIYTIRDQIILILGGENYLDTDQKWYFYYRVEGSKYPDKFWGIGDDAPRWAEE
                     DFTAWYGLIDITARYKVWGDLGIGLMYDFAGYDMRDMDDYTKETDPEGRKVPSPLRSG
                     TVPGSKGGYYNSLGPLLQYDSRDRVSWPSEGIYATLSLLPYHEYTGSSADFYKGELDF
                     RWYYSIIEGHVFSLQWDTVLSEGDVPFTMMPKLGGRDKLRGLPEGRYRDRNMTLLQLE
                     MRIPVVWRFGAVFFASAGNVWDRFEDFHADRITYGGGLGVRLTVDKDEKVNARADLGI
                     TKEGWAIYFYLMEAF"
     gene            2435..3655
                     /locus_tag="PKH10_06460"
     CDS             2435..3655
                     /locus_tag="PKH10_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT27802.1"
                     /translation="MERRRFDVVIVGAGPAGLMAAAHLPAGVKAIVLEKGTRPGRKLL
                     ISGSGQCNLTAVEDIAEFERHYGRNGRWLRPALRNFTNRDLVLFFERRGVPCLCTAEG
                     KYFPASLRAADILDALRSGLRRTGMLLQGDRPVERIVPVERWLVIQAGGDTYETRAVV
                     VATGGKSYPQTGSTGDGQRLAAALGHTIVPLRPALAPVVPVRHPLAPLAGNSCEDAGL
                     TVWRGGRKVHAAQGAMLITHTGFSGPLILDAARYLAPDDILRISFMTVPRDRFLRDIV
                     AEVRGRGAVSVKSMLKGAALTKNLREALIRQAGVDPDGRAADLSRDRLAALADAFCAF
                     PVTVRSLGGFETAMATAGGVSLDEVDPKTMMSKIVPGLFFAGEVLDIDGDTGGYNLQA
                     AFSTGALAGRSIKI"
     gene            complement(3670..>5088)
                     /locus_tag="PKH10_06465"
     CDS             complement(3670..>5088)
                     /locus_tag="PKH10_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT27803.1"
                     /translation="VILATAFLLVSSRRISRDIPLSRPFVFPLLFLSVPLGFVWYELF
                     PLRALLARHFESGTFPTNAAIASFLMLNLIAIIAAWLADRIPRHIGIYQARHDLFTAS
                     FRTRAAAATTHRELLRLFNTTLTSSFPEIRDLRYILFSEDDDHLPEAGLLRTCDPRLE
                     EGLIVEWFSDGSEDHLLRHAAPSGLLEEECDRIGADLVIPLRDKGMLFGILAINGVGI
                     NAAVARTIATIASIAADHYLRLSLLSVILHKERQIRETEYFAETGNMVSLIAHEIRTP
                     LTSVMLNLEVLKESLQKEGPIDPEYLDIAHREVKRLNETVEKMLLFGRNIKLEPTDGS
                     FDDLFADLRRTYATASVRFDAFPEGRFHLDWDRLRYLTINLVNNALQAIEQSGRPGEV
                     TVAVREEDGELILDVADTGPGIPEEKLSRIFDPFFTTRKEGNGLGLAICRKIARLMEG
                     TIELAVSRPGETRFTLTIPTEK"
BASE COUNT         1070 a         1398 c         1561 g         1059 t
ORIGIN      
        1 cggatctcga ccccaaaata ctccagaact cctatgacta tattctgacg ccgtattgtt
       61 gggatacctt ctcttccgag ttcccgatat attctttgac ggggtcgaca ctctctttct
      121 attctagtac cgcatcgcca tatgatggtg gtgattttat ctacgatctt gattccagaa
      181 aacttacgcg aattggacga ggaatggggg tttctccttt ttctaacgga gagtactttt
      241 tttctagaac aactgactac tctacagtgt gggaagaaag ttatgaagaa gtagttttct
      301 actatcactt tggaaataag acttacggga acgcatggaa gatgcctcag aaactctcgt
      361 atgtgcgaaa cattgtcgct agcgatcagt atatagccat gaatcttgtg cctctcacgg
      421 gcaatcgcta tgaacctctg cgcggatact acactaagat tggcgagtgg gacaaatggg
      481 tcgaattgaa tttcgggacc tacccatcct atggtatcgg aaagccggaa atacaaggaa
      541 gttatttggt ttcctataac tacattgaca atctcttacg agtgtttttc tgtgatctcg
      601 ccaaaggcga atcgagttgc aggatcgtga gcaaagcggg agagatcgcc cgtaaaccga
      661 tatggggggc caagaacact ttttactact ccgttgaaga ggaaaacgac ggagcaacag
      721 tttatcgaag cacaatagga ttcgttgatg gtctaggcac aaagctgatt tctttgccta
      781 ttttctcatc ggacaaatat atgcaggtat accaagcaag cgaagatttt cttctctatc
      841 ttaagtatat aaaagaaggt agtcctttcg acatatacga tgtttgttat tatcgttttt
      901 ccgacaaaaa acatttctgt ctggaagacg gcattaacag cgatattgaa tatcaagccc
      961 cgtattttta cgacaaatgg atcgtttttc agtcaaaata cgacttgata atacgagaca
     1021 tggacggcta ctgtcaggag catcctgaga attgcccgtt cgagccgtaa tcgcatccaa
     1081 ctaaaatcat tagactagcc gcttctatct gtcgcaatac tcaaatagcg ccataacccc
     1141 cgcatctctt taaaatcatt gatttcaggt tccatgtccg tatagtgagc ccactttttg
     1201 ctcggagtgc catgcgtccc gcgaccagta tcgtctttat gatcgctctt tttgctacgc
     1261 cgttcctcta cgccctcgag ccggtccccg ccggcgccac gcaggatctc atcgagaaga
     1321 aggagcaggc cgaggagaag aaggccgagg acgagaaggt cgaggagaag aagccggaag
     1381 agaaaaaaga gaagccgcgc aacgactaca tcatcatccc cgccatctac tacacccccg
     1441 agacatcggt cgccttcggt ttgtcggttc tgttctacta ccggccaccc aaccaactgc
     1501 ttgagcggcg ccccgccgtc atccagccca ccgtcatcta caccatccgc gaccagatca
     1561 tcctgatact cggcggcgag aactacctcg acaccgacca gaaatggtat ttctactacc
     1621 gcgtcgaggg aagcaagtac cccgacaagt tctggggcat cggcgacgat gcgccgcgat
     1681 gggccgaaga ggatttcacc gcgtggtacg gactcatcga catcacggcg cgttacaagg
     1741 tgtggggcga tctcggcatc ggtctcatgt acgatttcgc cggctacgac atgcgcgaca
     1801 tggatgacta caccaaggag accgaccccg aggggcgcaa ggtgccatcg ccgctccgga
     1861 gcgggacggt gccgggaagc aaggggggct attacaacag cctcgggccg cttctgcagt
     1921 acgacagccg cgaccgtgtc tcgtggccct cggaaggtat ttacgcgacc ctttcgctcc
     1981 tgccgtacca cgaatacacc ggcagttcgg ccgatttcta taaaggcgaa ctcgacttcc
     2041 ggtggtacta cagcatcatc gagggccatg tgttctcgct tcagtgggac acggtgctct
     2101 ccgagggcga tgttcccttc acgatgatgc cgaaacttgg cggccgcgac aaactgcggg
     2161 ggctccccga gggacggtat cgcgaccgta acatgacgct tctccaactt gagatgcgca
     2221 tcccggtcgt ctggcggttc ggcgccgtat tcttcgcatc ggcagggaat gtctgggacc
     2281 ggttcgagga ttttcacgcc gaccggataa cctacggcgg cgggttggga gtgcggctca
     2341 cggtcgacaa ggatgaaaag gtcaacgcgc gggccgacct cggtatcacc aaggaggggt
     2401 gggcgatcta tttctatctc atggaagcgt tctgatggaa cggcgccggt tcgatgtggt
     2461 catcgtcggc gcgggcccgg cgggactcat ggcggcggcc catcttccgg cgggtgttaa
     2521 ggcgatcgtg ctcgaaaagg gtacgcgccc cgggcgcaaa ctgcttatct ccggttccgg
     2581 gcagtgcaac ctcactgcgg tcgaggatat cgccgaattc gaacggcatt acggtcggaa
     2641 cggtaggtgg ctccggcctg ccctgcgcaa cttcaccaac cgcgaccttg tcctcttctt
     2701 cgagcggcgc ggcgtgccct gtctttgcac cgccgagggc aaatattttc ccgcctccct
     2761 gcgggcagcc gacattctcg acgcgttgcg gagtggactg cggcgcacgg gaatgcttct
     2821 gcagggggat cgtccggtcg aacggatcgt gccggtcgag cgatggttgg tcatacaggc
     2881 gggaggcgac acctatgaaa cgcgtgccgt cgtcgtcgcg acgggcggaa agagttaccc
     2941 gcagaccggg tcgaccggcg acggtcaacg gctcgccgcc gcgctcggcc atacgatcgt
     3001 gccactccgc cccgcgctcg cgccggtcgt gccggtacgg cacccgctcg cgccgctcgc
     3061 cgggaattcc tgtgaggatg ctggtctcac ggtatggcgc ggcggcagga aggtccacgc
     3121 ggcgcaaggc gcgatgctca tcacccacac cggtttctcg gggccgctca tactcgacgc
     3181 ggcccgttac cttgcgccgg acgacatcct gcggatatcg ttcatgaccg ttccccgcga
     3241 ccgtttcctg cgcgacatcg tcgctgaggt gcgtggcagg ggcgccgtct cggtgaagag
     3301 catgctgaaa ggggcggcgc tgaccaagaa cctgcgcgag gcgctcataa ggcaggccgg
     3361 cgtcgacccc gacggtcggg cggccgacct ttcgcgtgac cggctcgcgg cgctcgccga
     3421 tgctttttgc gcgtttcccg ttacggtgcg atcgctcggc ggcttcgaga cggcgatggc
     3481 gacggcgggg ggcgtgtcgc tcgacgaggt cgatccgaag acgatgatgt cgaagatcgt
     3541 gccgggactc ttcttcgcgg gcgaggtgct cgacatcgac ggcgacaccg gcggctacaa
     3601 cctgcaggcg gccttctcga ccggcgcgct cgccggaagg tcgatcaaga tataaaaaaa
     3661 aggaaggatt tatttttccg tcgggatggt aagcgtgaac cgggtctcgc cggggcgcga
     3721 caccgcgagt tctatcgttc cttccatgag ccgcgctatc ttgcggcaga tggcgaggcc
     3781 gagcccgttg ccctccttac gggtggtgaa gaaaggatcg aatatccgcg atagcttctc
     3841 ttcgggtata ccggggccgg tgtcggcgac atcgaggatc agttcaccgt cctcttcgcg
     3901 gacggcgacg gtcacctcgc cgggtctgcc cgactgctcg atcgcctgca gggcattgtt
     3961 gacgaggttt atcgtcaggt agcgcaggcg atcccagtcg agatgaaagc gcccctccgg
     4021 gaaggcgtcg aatcgcaccg aggcggtcgc gtaggtgcgg cgaaggtcgg cgaataggtc
     4081 gtcgaacgag ccgtcggtcg gctcgagttt tatgttgcga ccgaacagga gcatcttctc
     4141 gaccgtctcg ttgagtcgtt tcacctcgcg gtgggcgatg tcgagatatt ccgggtctat
     4201 cggtccctct ttctggagcg attccttcag cacctcaagg ttcaacatca ccgaagtgag
     4261 cggtgtgcgt atctcatggg cgatgaggga taccatgttg cccgtctcgg cgaaatattc
     4321 ggtctcgcgt atctgtcgtt ccttgtggag gatgaccgac agcagcgaga gccgcagata
     4381 atggtcggcg gctatcgacg cgatcgtcgc gatggtgcgg gcgacggcgg cgttgatgcc
     4441 gacgccgttg atggcgagta taccgaaaag catgcccttg tcgcggagcg gtatcacgag
     4501 atcggcaccg atgcggtcgc attcctcctc cagcaggccg gacggggcgg cgtgacgcag
     4561 gagatgatcc tccgaaccgt cggagaacca ttcgacgatg agcccctcct cgaggcgcgg
     4621 gtcgcaggtc cggagcaatc ccgcctcagg aaggtggtcg tcgtcctcac tgaagaggat
     4681 gtagcgaagg tcccgtatct cggggaagga agaggtgagc gtcgtattga aaaggcgcag
     4741 aagttcgcgg tgggtggtcg cggcagcggc gcgggtgcgg aacgatgcgg tgaaaaggtc
     4801 gtgccgggcc tgatagatgc cgatgtggcg cgggatgcgg tcggcaagcc acgcggcgat
     4861 gatcgcgatc aggttgagca tgaggaagga ggcgatggcg gcgttcgtcg gaaaggtgcc
     4921 tgattcaaaa tggcgggcga gcagggcgcg taacgggaac agttcatacc agacaaagcc
     4981 gagagggacc gaaaggaaga ggagggggaa gacgaagggg cgagacagcg ggatgtcgcg
     5041 ggatatgcgg cgggaggaaa cgagcaggaa ggcggtcgca aggatcac
//
LOCUS       DAOQUE010000173         5058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18299_length_5058_cov_4.675195, whole genome shotgun sequence.
ACCESSION   DAOQUE010000173 DAOQUE010000000
VERSION     DAOQUE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18299_length_5058_cov_4.675195"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKH10_06470"
     CDS             complement(<1..346)
                     /locus_tag="PKH10_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27804.1"
                     /translation="MKTKTLFFALMALLSFCTCTEKNEAKTDNEVVSDTDSATSDADA
                     VTGDELLSDADTWDFWKNGPRPPDWGPCTGGWEQKEQYNEETGRLEARWCEPPANWRP
                     AAPVDWGTCPEGW"
     gene            complement(368..2563)
                     /locus_tag="PKH10_06475"
     CDS             complement(368..2563)
                     /locus_tag="PKH10_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT27805.1"
                     /translation="MCKLRVLFLSILFLVLGACTEKSTTSTDDAATGDTDVAGDELLS
                     DADTYDFWKDGPQRPHPVDWGTCPEGWEPKEEKDEDGNLLAKYCEPILPPDDTECEPG
                     YFAMFGSTECQRIGDPCPEGEFADIPTDAGTEIIYVSEGESIQAAIDAAQDGAVIAIG
                     KGTFTEKESYPGDWSDGNPRDAAISISSYPSKSITLWGACPEKTILRSDTPPLEGLFS
                     AIIMIKQYDPSSRVLVRDITVSGNRIGIAVKASSSNEDNFLVRRTSLVELENVAIVDA
                     KETGLYIRGSARVFFTNGLIRNTVPDDWGLGMGIDIDESASLVASRLIVEKSRSTGIK
                     MDSSDEVFGSLLIARDLIVRDTLSRESDQGNTNGEGIIIGNHVTADLSRVLIEQNRNF
                     GIIAVNASGVKQKITAKDIIIRDTLPEESSSISGFGLAIADSDELYDTGATEVKINNA
                     LIEKSHSVGLYAGSLEGGETWLMATDLIVRDTYPNMADSGVGSGMELSDNSTIVLERT
                     LIERNKTFGMAAYTEKENSYVDLFVIDLRVLDTESQQSDYRWGDGLVLMDNVTAEINR
                     TILKNNREIGMLINNSSLFGSPLVTLRNVLISNTNIRKCYELKLDCGYAPDMPFGHGL
                     GIYDGAEAFLSNVVVDGNQHGLQIKNAKVFTGENCSLPFGDEEGKTICVHILDNETAI
                     NAFDLPADYDVFAAFANTSTWYEGNGTPFSGDPEDVPEPPDPTNNPMEE"
     gene            complement(2612..4423)
                     /locus_tag="PKH10_06480"
     CDS             complement(2612..4423)
                     /locus_tag="PKH10_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27806.1"
                     /translation="MRALRSFVLASLVLVAVVSCGPDDKKEGVQGSYDLPASCENSCA
                     EVSTVVALDKELKSGTAACVCVRRGTYEGAFTVGHPVTVIGEKDNTSILTQEGGVTVT
                     VAADSVTLQDLVIRTAGKKTTGILAEGSWETQKLKDLKIDSCTVEATDGTGVKINNAE
                     GIIVQNGIIVQNGIVVQGDAVDQTDQGVGLSVSDSKTVTVSGGSITVNGGRSAAAFSV
                     TTGIIIQNGIIVQNGIIVQNGTTPISLLAAGVEDPPYSYGIYLKDCAEVSISNASFVN
                     DGISVTGANSWSLVALSSNSVSVASSSFNGADKPNTTACYAAASTIAINLSTVSRFTK
                     GGYVFKEGSIATMTGGELSSLKGDVAVYGSDSTITMTGTEILDIEPPSGAGSGGRGIA
                     LVSGAVIDAQGITVQNCRSSGIIFDDSFGTIAGATVTGNKYAGVWVQDAVATGTDKIS
                     ITNSEFDENDFVAVYAIDSAGIEIKDTTLSQSVLKERNEIPMGDGLVFFGEALATAVD
                     AIVLDNVDFDDNGRAGAIFDSGRINEPELIGVTANDLTVTGTVTDGKYGIIIQNGKPT
                     TAFDIERKANNAFAEIDANFTGTLAVFGEGQKQSVSQ"
     gene            4668..>5058
                     /gene="rpsF"
                     /locus_tag="PKH10_06485"
     CDS             4668..>5058
                     /gene="rpsF"
                     /locus_tag="PKH10_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNT27807.1"
                     /translation="MKEKHFESTVILKPEGGSEAVRQFADRVNGIVEGMNGKIVSFQS
                     WGEKKLAYEIKKSMKGHYVYFDYVGTGALVAELERNFNNWEHVLKFMSARHNDETDPE
                     QLRANAKGVSILGAAGRSVRSGRSSWAG"
BASE COUNT         1086 a         1458 c         1303 g         1211 t
ORIGIN      
        1 cccatccctc gggacaggtg ccccaatcca ccggagcggc gggacgccag ttggctggcg
       61 gctcgcacca gcgcgcctca aggcgcccgg tttcttcgtt gtactgctcc ttctgctccc
      121 agccgccggt gcagggaccc cagtcgggag gccgaggccc attcttccag aaatcccatg
      181 tgtcagcgtc ggagagtagt tcatcaccgg tcacggcatc ggcgtcgctc gtagcgctgt
      241 cagtgtcgct taccacctca ttgtcggtct ttgcttcgtt tttttcagtg caggtgcaaa
      301 acgaaagcaa agccatcagc gcgaaaaaga gcgtcttcgt tttcatgatt ttcctcccct
      361 ttattcgcta ttcttccatc gggttattgg tcggatccgg aggctccggg acatcctcgg
      421 gatcaccaga aaaaggcgtg ccgttacctt cgtaccaagt actggtgttg gcaaaggcgg
      481 caaagacatc gtagtcggcg ggaagatcga aggcgttgat cgcggtctcg ttatcaagga
      541 tatggacaca tatggttttc ccttcctcat caccgaacgg cagggagcag ttctcgccgg
      601 tgaagacctt cgcgtttttt atctgtaacc catgctgatt cccgtcgacg accacattgc
      661 tgaggaatgc ttcggcacca tcatatatgc caagcccatg tccgaaaggc atatcgggag
      721 catacccaca atcaagtttc aactcatagc acttgcggat attggtattt gagatcagaa
      781 catttctaag tgttaccaac ggactcccga ataaagagga attattgatc aacataccga
      841 tctctcgatt attttttaaa atggtcctgt ttatctcggc ggtgacattg tccataagaa
      901 ccagaccgtc cccccatcga tagtcgcttt gttgagattc ggtgtcaagc actcgaagat
      961 ctatcacaaa aagatcgaca tatgagttct ctttttcagt gtatgctgcc atgccgaagg
     1021 tcttattacg ctcaataaga gttcgttcca atacgatggt cgaattatcg gacaactcca
     1081 ttcctgagcc cacgccgctg tccgccatgt tcggataagt atccctcacg atcaggtcgg
     1141 tggccatcag ccaggtctcc cctccctcta acgatccggc ataaagaccc acggaatggc
     1201 ttttctcaat aagggcgttg tttattttta cctctgtcgc acccgtatcg tacaactcat
     1261 ccgaatcggc aatggccagt ccaaagcccg aaattgaact gctttcctcg ggcaaggtgt
     1321 ctcgaattat gatatctttg gctgttatct tttgttttac cccagaggca ttgaccgcta
     1381 taattccaaa gtttcggttc tgctcgatca atactcgtga gagatctgcg gtcacatggt
     1441 tacctataat gatcccttct ccattggtat ttccctgatc cgactcacga gacaaggtat
     1501 cgcgcacgat caagtcccgg gcgatcaaga gcgagccgaa gacttcgtca gaagaatcca
     1561 tttttattcc cgtcgatctg cttttttcaa cgatgagacg cgaggcaacc aaggaggcac
     1621 tctcatcgat atcgatcccc atgcccaggc cccagtcatc gggcaccgtg ttgcgtatca
     1681 acccgttggt aaaaaaaacg cgggccgagc cccgaatata caatccggtt tccttagcat
     1741 caactatggc gacattttca agttcgacca aggaggtcct tcttaccaag aaattatctt
     1801 cgttcgacga acttgcctta acggctatcc ctatgcgatt ccccgaaaca gtaatgtccc
     1861 ttaccagaac tctgctggaa gggtcatatt gttttatcat gatgatcgcg gaaaaaagcc
     1921 cctctaacgg cggtgtatcg gaccggagaa tcgttttctc cggacaggct ccccataggg
     1981 taattgactt cgaggggtat gaggaaatgc ttattgccgc gtcacgagga ttgccgtcgc
     2041 tccaatctcc cggatacgat tctttctctg taaaggttcc ctttccaatc gcaatcaccg
     2101 caccgtcctg cgccgcgtct atcgccgcct gaatggactc cccttcggac acataaatta
     2161 tctcggtccc ggcatcagtt gggatgtcgg cgaattcgcc ttcgggacac gggtcgccta
     2221 tccgctggca ttcggttgag ccaaacatcg cgaaatagcc cggctcgcat tcggtatcat
     2281 caggtggcag tatcggttcg cagtacttgg cgagcagatt gccatcttca tctttctctt
     2341 cctttggttc ccatccttcg gggcaggtgc cccagtcgac cggatgcggc cgttgcggac
     2401 catccttcca gaagtcgtaa gtgtcggcat cggagagcag ttcatcgcca gcaacatcgg
     2461 tgtcgccggt ggccgcatcg tctgttgacg tcgtgctttt ttcggtgcaa gcgccaagca
     2521 ccaaaaacag tatagaaaga aagagaaccc ggagtttaca catgaaaaga cctcctctcc
     2581 tcggtcgata tgttacgcgg atctcttgtt gctactgaga gacactctgc ttctgtcctt
     2641 caccgaatac cgcaagagta ccggtaaagt tggcatcgat ctccgcaaag gcattgttcg
     2701 ctttgcgttc gatgtcaaac gctgtggtcg gtttgccatt ctggatgatg atgccgtatt
     2761 tgccgtccgt gaccgttccg gtgaccgtca ggtcgttggc ggttacgcct ataagttccg
     2821 gttcgttgat ccgaccggag tcgaatatcg cgccggcgcg accattgtcg tcgaaatcca
     2881 cattgtccag cacgatcgcg tcaacggcgg tggcaagcgc ctcaccgaag aagacaaggc
     2941 catcgcccat cgggatctca ttcctttcct tcagaacgct ctgggaaagc gtggtgtctt
     3001 ttatttcgat gccggccgaa tctatcgcat agacggcgac gaaatcgttc tcgtcgaatt
     3061 cggagttggt gatcgatatc ttatcggtgc cggtcgccac cgcgtcctgc acccaaactc
     3121 cggcatactt gttaccggtg acggtggcgc ccgctatcgt gccgaacgaa tcgtcgaaga
     3181 tgatgccgct cgaccggcag ttctgcaccg ttatcccctg cgcatcgatg accgcgccgc
     3241 tgacgagcgc aatgccgcga ccacccgatc ccgcgccgga gggcggctcg atgtcaagta
     3301 tctcggtacc ggtcatcgtg atggtcgaat cgctgccgta cacggcgaca tctcccttga
     3361 gcgacgagag ttctccgccg gtcattgttg cgatggagcc ctctttgaac acatagccac
     3421 ccttggtgaa gcgagagacc gtcgaaaggt ttatagctat ggtcgaggcg gcggcataac
     3481 acgccgtcgt atttggcttg tcggcaccat tgaaagaaga ggatgccacc gagaccgaat
     3541 tgctcgacag cgcgaccagc gaccaagagt tggcgccggt cacggaaatg ccatcattca
     3601 cgaacgaggc gttggatatc gatacctcgg cgcaatcctt cagatagatg ccgtacgaat
     3661 acggggggtc ttccaccccg gcggcgagaa gagatatcgg cgtcgtccca ttctgcacga
     3721 tgatgccgtt ctgcacgatg atgccatttt gtatgatgat accggtcgtg acactgaagg
     3781 cggccgccga tctgccgccg ttaacggtaa tggaaccgcc cgaaacggtc accgtcttgg
     3841 agtcggacac cgacagtccc accccctgat cggtctggtc caccgcatcg ccctgcacca
     3901 cgataccgtt ctgcacgatg atgccgttct gcacgatgat cccctcggcg ttgtttatct
     3961 tcacgccggt gccatcggtc gcctcgaccg tacaggaatc tatcttgagg tctttcagtt
     4021 tctgggtttc ccaactgccc tccgcgagta tgccggtcgt ctttttgcca gcggtccgga
     4081 tgacaaggtc ctgcagggtg accgagtcgg ccgccaccgt gacggtcacg ccgccctctt
     4141 gggtaaggat gctggtgttg tccttctcgc cgatgaccgt caccgggtgt ccgacggtga
     4201 aggctccctc gtaggtgccg cgcctgacac agacacaggc ggcggtgccg ctcttcagtt
     4261 ccttgtcgag cgccacgacc gtcgagacct ccgcgcagga attctcgcac gacgccggca
     4321 ggtcgtaaga accctgcacc ccctcctttt tatcgtccgg gccgcacgaa acgaccgcaa
     4381 caaggacaag ggacgcgaga acgaaagaac ggagcgcgcg catgatatcc tccttagttg
     4441 taagggacgc ttggaactcg cggtgattct accttaaacg gtgttgaaat caaactttat
     4501 tccttaccgt tctctctgac ctttcaacct gttatctcca ccgacagtaa aaaatcttgc
     4561 caaccggtct attctgacta tatttcatcc gttttcgctc gttgaacaac cggagcgttc
     4621 tccttgttca attgaaggat tggactttat acccgtaagg aggaaccatg aaagagaagc
     4681 atttcgagtc caccgtcatc ctcaagcccg agggcggctc ggaggcggtc agacagttcg
     4741 ccgaccgcgt caacggcatc gtcgagggga tgaacggtaa gatcgtgtca ttccagtcgt
     4801 ggggcgagaa gaaactcgcc tacgagatca agaaaagcat gaaaggccac tatgtctact
     4861 tcgactatgt gggcaccggc gcgctcgtcg ccgaacttga gcgcaatttc aacaactggg
     4921 agcatgtgct caagttcatg tcggcccgtc acaacgacga gaccgaccct gaacagttgc
     4981 gcgcgaacgc caagggggtg tcgatcctcg gcgccgcagg ccgttcggtc cgttcgggcc
     5041 gatcctcgtg ggcgggcc
//
LOCUS       DAOQUE010000174         5058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18302_length_5058_cov_3.762143, whole genome shotgun sequence.
ACCESSION   DAOQUE010000174 DAOQUE010000000
VERSION     DAOQUE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18302_length_5058_cov_3.762143"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="PKH10_06490"
     CDS             complement(<1..933)
                     /locus_tag="PKH10_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27808.1"
                     /translation="MRIELVLLALLAMIALIGCKGNEATPEEDQDINDADISPCDTDL
                     TDSDTPSDCDGCAFPDDDADITCGSLTEEFFADMPVEWEAYFTLKMSGVINDVDSQDF
                     DMAFLAKVGVVLQGTTYNLADSYGVYMRDEVQDTEGNAHPAVVAVGIGNLTWLVADKL
                     ASIRLGQTAILVEDLLAWKAEQEADGYGDVTLSGTVQVIVFESWIEIVTSIDQRTRME
                     CVRGLSAMNGEETAYEGKMYFCWQENTQWAVGEQMKMMQYSKMIDDPVALLAVMNEGL
                     TSEDPEYRTDLCRCWEQDGVTPMDCEAMKAEFGLD"
     gene            1095..2240
                     /locus_tag="PKH10_06495"
     CDS             1095..2240
                     /locus_tag="PKH10_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNT27809.1"
                     /translation="MTEHFFASCPRGLETVLSDELAALGAGGVRLDEGGVHFEGDLSL
                     CYRANLHSRIASRILWRISVSDYHAEQDIYDTASALPWEAWFAVTRTIRVNMVGIKCP
                     LKSLNFAGLRIKDAVCDRFRRLTGERPSVDTANPDIRIHGLLEESRMTLSLDTSGDTL
                     FKRGVRRHTGAAPLRENLAAGILALAGWKPGIPLLDPMCGSGTFLIEAAQMALNIAPG
                     ISRSFAFQKLINFDAKSWKDISAQAIAAEREEGTLPIFGSDISAEALETARQNLEEAG
                     LAEAVTLNRADVREVTAPAAEGILVANLPYGERMGDKAALQELYPQLGDALKKRFSGW
                     DAYLFTADLQMAKSIRLATSRRIPLFNGALECRLFAYKMVAGRNQKS"
     gene            complement(2237..2428)
                     /locus_tag="PKH10_06500"
     CDS             complement(2237..2428)
                     /locus_tag="PKH10_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27810.1"
                     /translation="MCPLRTPTNIAVIVSFFALFIVLDIARRFVKDPLAAKVLRIVDT
                     ALYVAAFLFVASLLLRRFL"
     gene            complement(2432..3004)
                     /locus_tag="PKH10_06505"
     CDS             complement(2432..3004)
                     /locus_tag="PKH10_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT27811.1"
                     /translation="MARNFCCEILPEIMKRWSMRALSPDPLPNYDITAICEAARYAPS
                     CFNEQPWRFMVATVPIDLERFRSFLAPKNRRWAGVAPVLLLIIAKKNFAHNGKPNRWS
                     SFDTGTAWGYLTLEAWRRGIVAHGMGGFDAEKVRSELKIPDDHDIIAMAALGYPGDPA
                     KLAEEFRESEKPGERRPLPDTLLSPDHFMR"
     gene            complement(3013..4755)
                     /locus_tag="PKH10_06510"
     CDS             complement(3013..4755)
                     /locus_tag="PKH10_06510"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNT27812.1"
                     /translation="MKCPVFVTILMAFFLAGATDLPVLSKMVSPLQRIGVIDEQTPLI
                     VNGDETDYEIYQSAVGIMVDGSLCTASLIDPEVLLTAGHCVYLYEGNQLIIDAMSTPE
                     NVTVKAGANIMYGGTTIAKAVEVIKHSSWEGDLAWNAIDLALIHLDRKVTTLDPFPVR
                     VAPSEQEDDTGVIVGYGITSTGGYDSGVHRYGNTTILDLVSGTKALIEIGDPAGACQG
                     DSGGPFLTRQFDKDVVSGVASFVTGECSATSGSYYTWVLQYRDWIESVVVDLTGHGFV
                     SEEICGDDNDICDPGEQTPCDQVDAHYAGTTNAICNDGCYWWDTSACIPNCGDGYVML
                     PELCDGSGTDCASLGQYQSGTSAPCKNDCTGYDTSVCTATDCGDGVKEGNEFCDTQIT
                     SCETLGTYPENYYARCNDACTGYLIEDCTGGLIICGNGTIDPFEECDDSNTTSGDGCS
                     SKCKNEVTPDVDTVSPDDTDVDADAAVPVDEDGIVLPDAPTDDDVVAVDETPVPDEVS
                     PDTIAVVDDDAATGPECGNGILEEGEECDDGNMKPADGCNPDCSLPDVTPDVDTKPFR
                     MVASDGCSCAVISF"
BASE COUNT         1022 a         1591 c         1445 g         1000 t
ORIGIN      
        1 atcgaggccg aactccgcct tcatcgcctc gcagtccatc ggcgtcacac cgtcctgttc
       61 ccagcagcgg cagagatcgg tgcgatactc ggggtcttcg ctcgtcaacc cttcattcat
      121 caccgcgaga agtgcgaccg ggtcgtcgat catcttggag tactgcatca tcttcatctg
      181 ctcgccgacc gcccactgag tattctcctg ccaacagaaa tacatcttgc cctcgtaggc
      241 ggtctcctcg ccgttcatcg ccgacaggcc acgtacacac tccatacggg tccgttgatc
      301 gatcgaagtg actatttcta tccaagactc gaacacgatg acctgcacgg tccccgataa
      361 ggtcacatcg ccgtaaccat cagcttcctg ttccgctttc cacgccagca ggtcctccac
      421 gaggatcgcg gtctgcccaa gccggatcga agcaagtttg tcggcaacga gccaggtcag
      481 attgccgatg cctaccgcga cgacggcggg atgggcattc ccctcggtat cctgcacctc
      541 atcgcgcata taaacgccgt aactgtcggc caaattgtaa gtcgtcccct gaaggaccac
      601 cccgaccttg gcaagaaaag ccatatcgaa atcctggctg tcgacatcat tgatgacccc
      661 cgacatcttg agtgtgaagt atgcctccca ctcgacgggc atatctgcaa aaaactcttc
      721 ggtcaacgaa ccgcaggtga tatcagcatc atcatccggg aaagcgcatc catcgcaatc
      781 cgacggggtg tcactgtctg tcagatcggt atcacaggga gagatatccg catcgttgat
      841 atcctgatcc tcttccggcg tcgcttcgtt acccttgcat ccgatgaggg cgatcatcgc
      901 caacagagcg agaagaacaa gttcgattct catggcattc cccgttttgt ggactttaga
      961 tctgggacaa ttgtaacaaa ctcaatttct attgcaatag gagggaccga cttcttttag
     1021 cgatgacatt accttgactc gttcgcccat atcccctact atcgcccaca gtgaaaacgg
     1081 aaccggtacc cttgatgacc gaacatttct tcgcatcatg cccgcgcgga ttggagacgg
     1141 tcctcagtga cgaactggct gcgctcggcg ccggcggcgt tcgcctcgac gagggaggcg
     1201 tccacttcga gggcgacctg tcgctctgct accgtgccaa cctccacagc cgcatcgcga
     1261 gccgcatcct gtggcggata tcggtcagcg actatcacgc cgaacaggac atctacgaca
     1321 ccgcgtcggc cctgccgtgg gaggcatggt tcgccgtgac gcgcaccatc cgcgtcaata
     1381 tggtcggcat caagtgcccg ctgaaaagtc tgaatttcgc cggtctgcgg ataaaggacg
     1441 ccgtttgcga ccggttccgc cgtctcaccg gcgagcggcc gagcgtcgac acggcgaacc
     1501 ccgacatccg catccacggc ctgctggaag agtcgcgcat gacgctgtcg ctcgacactt
     1561 cgggcgatac gctgttcaaa cggggcgtcc ggcggcacac cggcgccgcc ccactccgcg
     1621 agaatctggc ggcgggcatc ctcgcgctcg ccggctggaa gccgggcatc cccctcctcg
     1681 acccgatgtg cggcagcggc acctttctca tcgaggcggc gcagatggcg ctcaatatcg
     1741 cgccgggcat ttcacgcagt ttcgccttcc agaagttgat taattttgac gcgaagagtt
     1801 ggaaggatat ctccgctcaa gcgatagcgg cagaaaggga agagggtacc ctgcccatct
     1861 tcggcagcga catctcggcc gaagcgctgg agacggcgcg acagaatctc gaagaggcgg
     1921 gactcgccga ggcggtgacg ctgaaccgcg ccgatgtccg cgaggtgacc gcaccggcgg
     1981 cggaagggat actcgtcgcc aacctcccct acggcgagcg gatgggcgac aaggcggcgt
     2041 tgcaggaact gtatcctcag ttgggcgacg cgctgaaaaa gcggttcagc ggctgggacg
     2101 cctatctttt caccgccgac ctgcagatgg cgaagtccat caggctggcg acctcgcgcc
     2161 gcatcccgct gttcaacggg gcgctcgaat gccgcctgtt cgcatacaag atggtggccg
     2221 ggcgcaatca gaagagctag aggaaccgcc gcaacagaag cgacgcgacg aacaggaacg
     2281 ccgcgacata gagcgcggtg tcgacgatgc gaagcacctt cgccgccagg gggtccttga
     2341 cgaagcgccg cgcgatgtca agcacgatga acagcgcgaa gaacgatacg atgacggcga
     2401 tgttggtcgg cgtccgtagc ggacacatga actacctcat aaagtggtcg ggagacagca
     2461 gggtatcggg aagcggcctg cgctcgccgg gcttctcgct ctcgcggaac tcctctgcca
     2521 gtttcgccgg gtcgcccgga tagccgagcg ccgccatggc gatgatgtcg tgatcgtcgg
     2581 gtatcttgag ttcgctgcgc actttctcgg cgtcgaaacc gcccatgccg tgggcaacga
     2641 tgccacgccg ccacgcctcg agcgtgagat acccccacgc cgtcccggtg tcgaacgacg
     2701 accagcggtt cggtttcccg ttgtgggcaa agttcttctt tgcgatgatg agcagaagca
     2761 ccggagcaac acccgcccat cgacggttct tcggcgcgag gaacgaccgg aaccgttcaa
     2821 ggtcgatggg gaccgtcgcg accatgaagc gccacggctg ctcattgaag cacgaggggg
     2881 catagcgcgc cgcctcgcag atggcggtga tatcatagtt cgggagcggg tcgggcgaca
     2941 gggcacgcat cgaccagcgc ttcatgatct ccggcagtat ctcgcagcag aagttccgcg
     3001 ccatgagcgg cctcagaagg aaatgaccgc gcacgaacag ccgtcggagg cgaccatccg
     3061 gaacggcttc gtatcgacat cgggcgtgac atcgggcagg gagcagtctg ggttgcaccc
     3121 gtcggccggc ttcatgttgc cgtcatcgca ctcctccccc tcttcgagta tgccgttccc
     3181 gcactcggga ccggtcgcag catcgtcgtc cacgaccgcg atcgtgtcgg gcgacacctc
     3241 gtcggggacc ggtgtctcat cgaccgcgac gacatcatca tccgtcggag cgtcggggag
     3301 aacgatcccg tcctcatcga caggcacggc ggcatcggca tcgacatcgg tatcgtcggg
     3361 cgaaaccgta tcgacatccg gcgtgacctc gttcttgcat ttcgaggaac agccgtctcc
     3421 cgatgtcgtg ttgctgtcgt cgcattcctc gaacgggtcg atagtgccgt tgccgcagat
     3481 gatgaggccg cccgtgcagt cctcgatgag gtagccggtg cacgcatcgt tgcaccgggc
     3541 gtaatagttc tcaggatagg tgccgagcgt ctcgcaactc gttatctggg tatcgcagaa
     3601 ctcgttacct tccttgacgc cgtcgccgca atcggtcgcc gtgcagaccg aggtgtcgta
     3661 cccggtgcag tcgttcttgc agggggcgct cgtccccgac tggtactgac cgagactcgc
     3721 acagtcggtc ccgctaccgt cgcaaagctc gggcaacatc acatatccgt caccgcagtt
     3781 gggtatgcac gccgaggtgt cccaccagta gcacccatcg ttgcagatcg cgttggtggt
     3841 gccggcgtag tgtgcatcaa cctgatcgca gggcgtctgc tcgcccgggt cgcagatgtc
     3901 gttgtcgtcg ccgcagatct cctcgctcac gaacccgtga ccggtgagat ccacgaccac
     3961 cgactctatc caatcgcgat actggagcac ccatgtgtaa taactgccgc tcgtggccga
     4021 acactctccc gtcacgaacg aggcgacgcc cgacacgaca tccttatcga actgccgggt
     4081 caggaacggg ccgcccgaat cgccctgaca ggcccccgcc ggatcgccga tctcgatgag
     4141 tgccttggtg ccgctcacca gatcaaggat ggtggtgttg ccatagcgat ggacgcccga
     4201 atcatagccg ccggtgctgg tgatgccgta gccgacgatg acgccggtat cgtcctcctg
     4261 ctcactgggc gcgacccgga ccgggaacgg atcgagcgtc gtcaccttgc ggtcgagatg
     4321 gatgagcgca agatcgatcg cgttccatgc aagatcgccc tcccaactcg aatgtttgat
     4381 gacctcgacc gccttggcga ttgtcgtacc gccgtacatt atgttcgctc ccgccttcac
     4441 ggtgacattc tcgggcgtcg acattgcatc gatgatcaac tgattgccct cgtacaggta
     4501 cacgcagtgt cccgccgtga gcagcacctc ggggtcgatg agactcgcgg tgcagagcga
     4561 cccgtccacc atgatgccga cggcgctctg gtatatctcg taatcggtct cgtccccgtt
     4621 gacgatgagt ggggtctgct catcgatcac gccgatcctt tgcagggggc tgaccatctt
     4681 cgagagaacg gggagatctg tcgcccccgc aagaaaaaag gccatgagga tcgtaacgaa
     4741 aaccgggcat ttcatgtgga ccatctccct acattgtctc tatcaagatg accattctat
     4801 cctgttctct ttgaaaagca aactcaccat cctttcaaaa gaagcaaccc ggcaaccgaa
     4861 catttttcct aaacaaactc gatagtttta caaatgaact gacatgtcag tctagttttc
     4921 ttgacttcgc ttttcttttc cgctatcatc ggcccgttac ctgatcgttg atccgttgaa
     4981 agggaggaga ggatgatcga aacaaaggta cggaacacta ttacggcgac cgccgacctt
     5041 atcaccgaga tgttctcg
//
LOCUS       DAOQUE010000175         5050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18344_length_5050_cov_4.321922, whole genome shotgun sequence.
ACCESSION   DAOQUE010000175 DAOQUE010000000
VERSION     DAOQUE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18344_length_5050_cov_4.321922"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1488)
                     /gene="murA"
                     /locus_tag="PKH10_06515"
     CDS             complement(202..1488)
                     /gene="murA"
                     /locus_tag="PKH10_06515"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNT27813.1"
                     /translation="MEYFDIHGGGPVTGEVTLSGNKNEALPVLAATLLTDEPVVLRNV
                     PDIIDVRIMLEIMERLGVSVDRPEPHRLVICARGVTDSPLDRELCARIRTSFLFVGPL
                     LSRWRRVEVPLPGGDIIGRRRLDTHFLGFTQLGALCEISGEGYRFSSKGLRGAEIHLD
                     EASVTGTENILMAAVCAEGRTVIHNAACEPHVQGLISFLNAMGARIDGAGTNRLTIEG
                     VRSLAGADHTIGPDHIEAGSFIALAAVTGGSLTVKDAAPQHLHTIRRIFQTVGIETRV
                     NGQDIVVPPRQGMSVRKDLHNAVPRIDDGPWPSFPSDLMSIAIVAATRAEGTLLFFEK
                     MFESRLYFVDRLIQMGAQIILCDPHRVVVSGPSVLYGATLESPDIRAGMALLIAAAAA
                     QGRSRIHNIRQIDRGYERIEEKLRKIGITIDRCKLK"
     gene            1614..1689
                     /locus_tag="PKH10_06520"
     tRNA            1614..1689
                     /locus_tag="PKH10_06520"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1647..1649,aa:Ile,seq:cat)
     gene            1849..2631
                     /locus_tag="PKH10_06525"
     CDS             1849..2631
                     /locus_tag="PKH10_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNT27814.1"
                     /translation="MLEELKKLKKLQKCDELIFQRETVLDALPEENERLEQQIRELGN
                     KLTDLRGGVSLHEDERRKREDILNRGEEKLKGITGKQSAIRNKEEYNALLREIDNIKR
                     FNKEISEEVSEIDREIEIKKQELELIEQENLKKIQEAKQKLAKNLEQIKSIEHEVEKK
                     YQERDKLAATIRPAILRKYERILESSSNGRVIAETENYICRGCNMTLPPQLYNNVLKA
                     ERIETCPNCQCILIPTEGRKVDKKSGPVVRDTAERPSEEEDE"
     gene            2636..2989
                     /gene="rnpB"
                     /locus_tag="PKH10_06530"
     ncRNA           2636..2989
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKH10_06530"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3075..3782
                     /gene="pyrH"
                     /locus_tag="PKH10_06535"
     CDS             3075..3782
                     /gene="pyrH"
                     /locus_tag="PKH10_06535"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNT27815.1"
                     /translation="MSRRILLKISGEALLAPDGSPICTSFLDFLAGEVGAVTAKGIQV
                     AIVVGGGNIFRGLQESGKTGIDRATGDYMGMLATVINALALQAAFEKSGLPTRVQSAI
                     EVNKIAEPYIRRRAIRHLEKGRIVIFGAGTGNPFFTTDTAAALRAVEIGADLLIKATK
                     VDGLYDKDPAKHKNAAFIKKATFHEAIEKGYKVMDASALTLCAENELAIKIVNIYTPG
                     NILSATISDEVGSLITV"
     gene            3808..4371
                     /gene="frr"
                     /locus_tag="PKH10_06540"
     CDS             3808..4371
                     /gene="frr"
                     /locus_tag="PKH10_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNT27816.1"
                     /translation="MFDQKTFFDHLKEKFQKSLDSYKMELAAFRTGKASPAILDSVHI
                     NYYGQKTPLNQAASISSPDARMLVVQPWDKTQLKEIETAIRSANLGLNPINDGHFIKV
                     PIPSLTEERRKEIVKQIHQATERFRVSQRHIRRDAIDEIKKAQKDKAITEDDEKKYTE
                     LVEKELKNFLKSLDEMAAKKEKEVMEI"
     gene            4374..>5050
                     /locus_tag="PKH10_06545"
     CDS             4374..>5050
                     /locus_tag="PKH10_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNT27817.1"
                     /translation="MTGPVLGIDLGTTTTIASVIRDGIPEVIPVEGGSRFLIPSVVHF
                     GENGEVLSVGTEAKKQKLPSPTRTVFSIKRIIGRTFSHPDTEVAIMRFPFPIVEGSND
                     SVLLNIGRKSYSPQEVSSFVLQKAIEYAHRHLGEPTDRVVITVPANFNEAQRRATQNA
                     GKLAGLDVLRIINEPTAAALAYGFGNTANERVAVYDLGGGTFDISILETRNKVLDVLS
                     TGGNSFLG"
BASE COUNT         1193 a         1524 c         1459 g          874 t
ORIGIN      
        1 cgccttgcgc gccagttccg acttgctcaa aagaagtttc tctcgatact ttttcagcat
       61 gttctttacc atgtccctct ccttgatcgg ggtcgttttc caaggccgat ggtaacatgc
      121 gcgttttgca aaatcaagat ttttttgctt aatttcatat cattgttata taactatatg
      181 ccactgatat aaaaaacata gtcatttcaa cttacaacgg tctatagtta tgcctatctt
      241 tctcaatttt tcctctatcc gctcgtaccc ccggtctatc tgccggatgt tgtggatgcg
      301 gctccgcccc tgcgcggcgg cggcggcgat cagaagcgcc ataccggccc ggatgtcggg
      361 agattcgagc gtggccccgt aaagaactga gggaccgctg acaacgaccc ggtgaggatc
      421 gcagaggatg atctgagcgc ccatctgaat aagccggtcc acgaagtaca ggcggctctc
      481 gaacatcttc tcgaaaaaga gaagcgtccc ctcggcgcgg gtcgccgcca ctatggctat
      541 ggacatgagg tcggagggga acgagggcca cggaccgtcg tcgatccggg gaacggcgtt
      601 gtgaaggtct ttcctcaccg acatgccttg tcgtgggggc accacgatat cctgcccgtt
      661 cacccgggtc tcgatgccga cggtctggaa gatgcgcctg atggtatgga gatgctgcgg
      721 ggccgcgtcc ttaacggtca gggagccgcc ggtcaccgcg gcgagcgcga taaaactgcc
      781 cgcctcgata tgatcggggc cgatggtatg gtcggcaccg gcgagcgacc gcacgccctc
      841 aatggtcagc cggttggtcc ccgctccgtc gatacgcgcc cccatggcgt tgaggaacga
      901 gatgagcccc tgcacatgag gctcacaggc ggcgttgtgt atgaccgtcc gtccctcggc
      961 gcatactgcg gccatcagga tgttctcggt ccccgtcacg ctcgcctcat cgagatgtat
     1021 ctcggcaccg cgaagacctt tcgatgagaa ccgataccct tctccgctta tctcgcagag
     1081 cgcaccaagt tgtgtgaaac cgaggaaatg ggtatcgagg cgacgacgtc cgatgatgtc
     1141 accgcccggg agcgggacct cgacccggcg ccagcgggaa aggagcggcc ccacgaacag
     1201 gaacgaggtc cggatgcggg cacagagttc ccggtcgagg gggctgtcgg tcacgccgcg
     1261 ggcgcagatg accagtctgt gcggctcggg gcgatccacc gaaacgccga gccgttccat
     1321 gatctccagc atgatccgca catcgatgat gtcggggacg ttgcggagaa cgaccggctc
     1381 gtcggtcaac agggtcgccg cgaggaccgg gagcgcctcg ttcttgttac ccgaaagggt
     1441 cacctctccg gtgaccggtc caccgccgtg tatgtcaaaa tattccatac aaggacacct
     1501 gtctcaatga ccatgaaaaa gggacacgaa acaatagtgg taatagtgac aaagtcaatt
     1561 ttaacaaaaa atttgatttt tcattttttg ccactagagt gggcccgaat tcggggccta
     1621 tagctcagtt ggtagagccc ccggctcata accggatggt cggaggttcg actcctcctg
     1681 ggcccaccat tatttacgga ggcgggcgat ctggctgctt ttttcacata cgacgatacc
     1741 ctcagaggtt atcccccctc ttcggcactt gtcttcaccg gcaagtgctt tttttttgcc
     1801 tcgacctcag gatctgtgcg atgatcaacc actgcaaagg agacacccgt gcttgaagaa
     1861 ctgaagaaac tgaagaaact tcagaaatgc gacgaactga tattccaaag ggaaacggtc
     1921 cttgacgccc ttccggaaga gaacgagcgg ctcgaacagc agatccgcga gttagggaac
     1981 aaactgaccg accttcgggg cggcgtctcc ctgcacgagg acgagcgacg gaaacgggag
     2041 gatatcctga accgcggcga ggagaaactc aaaggcatca ccggcaagca gtcggccata
     2101 cgcaacaaag aggagtacaa cgccctcctg cgcgagatag acaacatcaa gcgcttcaac
     2161 aaggagatat cggaggaggt atccgagata gaccgcgaga tagagataaa gaaacaggaa
     2221 ctggaactca tcgaacagga gaacctcaag aagatacagg aggcgaaaca gaaactcgcc
     2281 aagaacctcg aacagatcaa gagcatcgag cacgaggtcg agaagaaata tcaggagcgg
     2341 gacaaacttg ccgccaccat ccgaccggcc atactccgca aatacgagcg catactggaa
     2401 agttcttcca acggccgcgt catcgcggag accgagaact atatctgccg cggctgcaac
     2461 atgaccctgc cgccgcaact ctacaacaat gtgctcaagg ccgaacggat cgagacctgc
     2521 cccaactgcc agtgcatcct gataccgacc gaggggcgca aggtcgataa gaaaagcggc
     2581 ccggtcgtcc gcgacaccgc ggaacggccc tccgaggaag aagacgaata gcggggaagt
     2641 tgcacgaccg accgccggcg cgagccggag gaaagtccgg actccacaga gcgggaggct
     2701 ggctaacggc caggagaggc gactctacgg aaagtgcaac agaaagatac cgcccggcat
     2761 gaccgggcaa gggtgaaatg gcgaggcaag agctcaccgg gagcgcggtg acgcgcttcg
     2821 cacggcaaac cccctccgga gcaagatcga ataggggcgc gttccgcctt cgggcggata
     2881 agggttgctc gcccgagacg cccgggttga tcgctcgagg cggccggtaa cggtcgtccc
     2941 agaggaatgg tcggtctcga cagaatccgg cttatgtgcg acttcccctt ttcctgccgc
     3001 ttatgaaaac ttgaaaaagc attccttatc atatagggtg ggtggacctt actagacgca
     3061 gaagggaggc ccccatgtcc cgacgcatac ttctgaagat aagcggcgag gcgttactcg
     3121 cccccgatgg ctcccccatc tgcacctcgt tcctcgactt tctcgcgggc gaggtcggcg
     3181 ccgtgaccgc gaaggggata caggtcgcca tcgtggtcgg cggcggcaac atcttccgcg
     3241 gacttcagga atcgggaaaa acgggcatcg accgcgcgac cggcgactac atggggatgc
     3301 tcgccaccgt gataaacgcg ctcgccctgc aggcggcgtt cgagaaaagc ggcctgccga
     3361 cccgcgtgca gtcggccatc gaggtcaaca agatagccga gccctacatc cgccgccgcg
     3421 ccatccgcca cctcgagaag gggcgcatcg tgatattcgg cgccggcacc ggcaacccct
     3481 tcttcaccac cgacaccgcc gccgccctgc gcgccgtcga gatcggggcc gatctgctca
     3541 tcaaggcgac caaggtggac gggctctacg acaaggaccc ggcgaagcac aagaacgccg
     3601 cgttcatcaa aaaggcgacc ttccacgaag cgatcgagaa gggctacaag gtgatggatg
     3661 cctcggcgct cacgctgtgc gccgagaacg aactcgccat caagatcgtg aacatctaca
     3721 cgccgggcaa catattgagc gcgacgatct ccgatgaggt cggcagtctg ataaccgtgt
     3781 aggctgacag catagcagga ggcatccatg ttcgaccaga agaccttttt cgatcacctg
     3841 aaggaaaagt tccagaagtc gctcgactcc tacaagatgg aactcgccgc cttccgcacc
     3901 ggcaaggcgt cgcccgccat cctcgacagt gtccacatca actactacgg tcagaagacg
     3961 ccgctcaacc aggcggcctc catatcgagt cccgacgccc gcatgcttgt cgtccagccg
     4021 tgggacaaga cccagttgaa ggagatagag accgccatcc gcagcgcgaa tctcggactc
     4081 aacccgataa acgacggcca cttcataaag gtgccgatcc cgtcgctgac cgaggagcgc
     4141 cgcaaggaga tcgtcaaaca gatacatcag gcgaccgaga ggttccgcgt gagccagcgc
     4201 cacatccggc gcgacgcgat cgacgagata aagaaggccc agaaggataa ggcgatcacc
     4261 gaggacgacg agaagaaata taccgaactg gtcgagaagg aactcaagaa cttcctcaag
     4321 tcgctcgacg agatggccgc caagaaggaa aaagaggtca tggagatctg atcatgacgg
     4381 gaccggtgct cggcatagac ctcggtacga cgacgaccat cgcttcggtc atccgtgacg
     4441 gcatacccga ggtgatcccg gtcgaaggcg gttcgcggtt cctgataccc tcggtcgtcc
     4501 atttcggcga gaacggcgag gtgctgagcg tcgggaccga ggcgaagaag cagaaactcc
     4561 cctcccccac ccgcaccgtg ttctccatca aacgcatcat cgggcggacc ttctcgcacc
     4621 ccgacaccga ggtggcgatc atgcgcttcc ccttccccat cgtcgaggga agcaatgact
     4681 cggtgctcct caacatcgga cggaaaagct actcgccgca ggaggtttcg tccttcgtac
     4741 tccaaaaggc tatcgaatac gcgcatcgac acctcggcga accgaccgac cgcgtcgtga
     4801 taacggtacc cgcgaacttc aacgaagcac agcgacgcgc cacgcagaac gccgggaaac
     4861 tggcgggact cgatgtgctc cgcatcatca acgagccgac cgccgcggcg ctcgcctacg
     4921 ggttcggcaa caccgcgaac gaacgggtag ccgtctacga cctcggcggc ggcaccttcg
     4981 atatatcgat actcgagacg cgcaacaagg tgctcgatgt cctctccacc ggcggtaact
     5041 cgttcctcgg
//
LOCUS       DAOQUE010000176         5043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18375_length_5043_cov_4.143945, whole genome shotgun sequence.
ACCESSION   DAOQUE010000176 DAOQUE010000000
VERSION     DAOQUE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18375_length_5043_cov_4.143945"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..715)
                     /locus_tag="PKH10_06550"
     CDS             complement(86..715)
                     /locus_tag="PKH10_06550"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNT27818.1"
                     /translation="MFTGIIEKTGKVERLTASGPERTLTLDNPFGNDIKSGDSIAVDG
                     VCLTVTAFDAKSMSFFISAETVKKTLAAHYKPGRSVNLERALAFGGRLDGHIVTGHVD
                     TVSRIASVKKVGAGVELAVTIPPEFGHLTVDRGSIAVNGISLTIAEMRSEAAVVSLIP
                     ETQARTSFADGVKAGDAVNLEFDILGKYAARLLAPHLRDDKLRALIEKL"
     gene            852..4565
                     /locus_tag="PKH10_06555"
     CDS             852..4565
                     /locus_tag="PKH10_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNT27819.1"
                     /translation="MTNGSAHRLVISLARFGEKSPRSRPDGNARHFHVSRESRDRYRF
                     EERIFTLSGNVVFPDYAAVRRFAHAMNEKKDLVKHPEQAVKAGHLNAMALIDEVLHHI
                     VRIYRQQVAPTLLTEALSFLRERLGDDTDAAIRRFAEEFPPLPVYRGQMSITEYLAGN
                     CGDEPALEVELEELLMLHIANRNPAFKRYEELFSDEPLRRTTRYSELTAAFGEFLAQK
                     PAFGPDGQKLLDLLMSPAGAHPDSLADQLRFIRERWGLRFLGDRFLKVLSGGDLLREE
                     EKPYFPFEGRGEVITGESLRGGYIPGQWRDHEYEAFSPDRVWMPNLVMIAKNAFVWLD
                     QLSRQYGRHLTRLDDIPEDELKKLARWGFTGLWLIGVWERSTASRTIKQIMGNPEAAA
                     SAYSLHDYTIAQELGGEEALATLRDRAGRYGIRLASDMVPNHMAIDSRWVHHHPDWFL
                     QAKEPPFPSYTYNGPNLSTEEHLGIYIEDRYYDKTDAAVTFKRVDFRHDDVRYIYHGN
                     DGTSYPWNDTAQLDYTRADVREAVIQTILHVARQFPVIRFDAAMTLAKRHYQRLWFPE
                     PGTGGDIPSRTEYALTRDQFNERIPNEFWREMVDRVQQEQPDTLLLAEAFWLMEGYFV
                     RTLGMHRVYNSAFMNFLKKEENDQYRLSVRNVLEFDPDVLHRFVNFLNNPDEKTAVEQ
                     FGKDDKYFGVSLLMATMPGLPMFGHGQVEGFTEKYGMEYRKAYWNESVDHQLVQRHER
                     ELFPLLRKRYLFAGVENFRLYDFFLHTGRVDENVYAYSNRRDDERALILFNNKFSETS
                     GWIRTSVGFLDKGSGQVVQRTLVEGLGLTPGAGRYLLLRDHITGLEFIRRVDDLAETG
                     LFVTLGAYKYQAFLDLREVIETAAEPWGSLHDLLNGAGVPDIRRALTMLTVAPLHAAF
                     RRLANPATFKALIAARGAKGPVKMKATTRKLLLQLAVHHRSFLDELRLLHGGAGDVDI
                     LAGLFAHDLAAFIELPTVDLAPLHPPAQEWLTSVVGDDVSALLMMFGWTLTARLSQIT
                     LDRNDGPTSNEILRKYLLEEVFLTLFREMGRGERHADMEQRHIRAFVYYQNWWRQYPQ
                     DDLCHAMMKDVFADENMHRILQVNEYRGIRYFNKEAFEHFVNGLFVMAVLDILRSDLT
                     PAKKKKELATRTTLARRFLDAMPLSEYRVDKLLEVIKNPVKVPLTRDRKEEEGGWLIG
                     EPVFVPLTEPKRPVKKKKTPAKTKKTTAKRKKT"
     gene            4562..4795
                     /locus_tag="PKH10_06560"
     CDS             4562..4795
                     /locus_tag="PKH10_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27820.1"
                     /translation="MRFIAALIALLLAAAMTYYYITGSWPGMSKDPLKDASKKAAIEG
                     GPITDPQMAKDRVEGMLKKSEEAQRKAIEDSTQ"
     gene            4832..>5043
                     /locus_tag="PKH10_06565"
     CDS             4832..>5043
                     /locus_tag="PKH10_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27821.1"
                     /translation="MKRFIIIGVAALLVLTACAGTKTGDDPAPENDENATDTTVNDTT
                     VTDTAATDTAVPDDTVTDETTTDSDVV"
BASE COUNT         1019 a         1665 c         1464 g          895 t
ORIGIN      
        1 cgggacgaca aactgcgcgc cctcatcgag aaactatagc cacgagcgtt acggtcctga
       61 acatggggcc tcctcccgac cggggctata gtttctcgat gagggcgcgc agtttgtcgt
      121 cccgcaggtg cggggcgagg agccgcgccg cgtatttgcc gaggatgtcg aattcgaggt
      181 tcaccgcgtc ccctgccttc acgccgtcgg cgaacgaggt ccgcgcctgc gtttcgggga
      241 tgagcgatac gaccgccgcc tcgctgcgca tttcggctat cgtgagcgag atgccgttca
      301 ccgcgatgga gccgcgatcg accgtaagat ggccgaattc cggcggtatc gtcaccgcca
      361 gttcgacccc cgcgccgacc tttttgaccg aggcgatgcg cgacacggta tcgacatggc
      421 ccgtcacgat gtggccatca agccgcccgc cgaacgcgag cgcccgttcg agattgaccg
      481 accgccccgg cttgtagtgc gccgcgagcg tctttttcac cgtttcggcc gatatgaaga
      541 aactcatact tttcgcgtcg aacgccgtca ccgtcagaca gacaccgtcc acggcgatgc
      601 tgtcgccgct cttgatgtcg ttcccgaacg ggttgtcgag cgtgagcgtc cgctccggtc
      661 ccgacgcggt caaccgttcg accttacccg tcttttcgat gatgccggtg aacatagttc
      721 cttctctttg gtgcacgcgg cgcactatat ccgactagcg agcactttgc aagttattcg
      781 cccgccgcaa cattgacttg tccaccgctt tgcggtagtg ttcgccgttg caaatgaaca
      841 cggggtcccg catgacgaac ggttccgctc accgactggt catatcgctt gcgcgcttcg
      901 gcgaaaaaag cccccgctct cgtcccgacg ggaatgcgcg ccatttccat gtgtcgcgcg
      961 agagccgcga ccgctaccgg ttcgaggaac ggatcttcac cctgtcgggc aatgtggtct
     1021 tccccgacta cgccgcggtc cgccggttcg cccacgcgat gaacgagaaa aaggacctcg
     1081 tcaaacaccc cgaacaggcg gtgaaggcgg ggcatctcaa cgcgatggcg ctcatcgatg
     1141 aggtgcttca ccatatcgtg cggatttacc gccagcaggt ggcgccgacc ctgctcaccg
     1201 aggcgctctc ctttctgcgc gaacggctcg gcgacgacac cgacgcggcg atccgccgct
     1261 ttgccgagga gttcccgccc ctcccggtct accgcggcca gatgagcatc accgaatacc
     1321 tcgcagggaa ttgcggcgac gagccggcgc tcgaagtgga actggaagaa ctgctgatgc
     1381 tccatatcgc gaaccgcaac cccgccttca aacgctatga ggaacttttc agtgacgagc
     1441 cgctccgccg caccacccgg tacagcgaac tcactgcggc gttcggcgaa ttcctcgcgc
     1501 agaaaccggc attcggtcct gacgggcaga aactgctcga ccttctcatg tcgccggcag
     1561 gcgcccaccc cgattcgctc gccgaccagt tgcgcttcat ccgcgagcgg tggggactcc
     1621 ggttcctcgg cgaccggttc ctgaaggtgc tcagcggcgg cgacctgctc cgcgaggagg
     1681 agaaacccta ctttccgttc gagggtcgcg gcgaggtgat caccggtgaa tcccttcgcg
     1741 gcggatatat ccccggccag tggcgcgacc atgaatacga ggccttctca cctgatcgcg
     1801 tctggatgcc caacctcgtg atgatcgcca agaacgcctt cgtctggctc gaccagttgt
     1861 cccgccagta cggccggcat ctcacccggc ttgacgacat ccccgaggac gaactgaaaa
     1921 aactggcccg ctggggcttt accggcctgt ggctcatcgg cgtctgggag cggtcgaccg
     1981 cgagtcgcac cataaaacag atcatgggga accccgaagc ggccgcatcg gcctattcgc
     2041 tccacgacta cacgatagcg caggaactcg gcggcgagga ggcactcgcg accttgcgtg
     2101 accgggctgg gagatacggc atccgcctag ccagcgacat ggtccccaac cacatggcga
     2161 tcgattcgcg ctgggtccac caccaccccg actggttcct gcaggcgaag gagcccccct
     2221 tcccctccta cacctacaac ggcccgaacc tctcgaccga ggagcatctg ggcatctaca
     2281 tcgaagaccg ctactacgac aagaccgacg cggcggtcac cttcaagcgg gtcgatttcc
     2341 gccatgacga tgtccgctat atctaccacg gcaacgacgg caccagttac ccgtggaacg
     2401 ataccgcgca actcgactac acccgcgccg acgtccgcga ggcggtcatc cagaccatcc
     2461 tccatgtggc gcggcagttc ccggtgatcc ggttcgacgc cgcgatgacg ctcgccaaac
     2521 ggcactacca gcggctctgg ttcccggagc cggggaccgg cggcgacata ccgtcgcgca
     2581 ccgaatacgc cctgacccga gaccagttca acgagaggat accgaacgaa ttctggcgcg
     2641 agatggtcga ccgcgtccag caggaacagc ccgacaccct cctgctcgcc gaggcgttct
     2701 ggctcatgga gggctacttc gtccggacgc tcgggatgca ccgcgtctat aacagcgcat
     2761 tcatgaactt cctgaagaaa gaggagaacg atcagtaccg cctctcggtc cgcaatgtgc
     2821 tcgagttcga tcccgatgtg ctccatcggt tcgtcaattt cctcaataac cccgacgaga
     2881 agacggcggt cgagcagttc ggcaaggatg acaagtactt cggcgtctcc ctcctcatgg
     2941 ccacgatgcc cggcctgccg atgttcggcc acggacaggt cgagggattc accgagaaat
     3001 acggcatgga atatcgcaag gcctactgga acgagtcggt cgatcaccaa ctcgtccaac
     3061 ggcacgaacg ggaacttttc ccgctcctcc gcaagcggta tctgttcgcc ggcgtcgaga
     3121 acttccgtct ctacgacttt ttccttcata ccgggcgggt cgacgagaat gtctacgcct
     3181 actcgaaccg ccgggacgac gagcgggcgc tcatcctgtt caacaacaag ttctccgaga
     3241 cctcgggctg gatacgtacc tcggtcgggt tcctcgacaa ggggagtggg caggtggtcc
     3301 agcgaacgct cgtcgagggg ctcggactta ctcccggcgc aggacgctac ctgctgttgc
     3361 gcgaccatat caccggcctt gagttcatcc gacgggtgga cgatctggcc gagacggggc
     3421 ttttcgtcac gcttggtgcc tacaaatatc aggcattcct cgacctgcgc gaagtcatcg
     3481 aaacagccgc cgagccgtgg ggctccctcc acgacctcct gaacggcgcg ggggtccccg
     3541 atatccgccg ggcgctcacg atgctcaccg tggcgccgct ccacgccgcc ttccgccggc
     3601 tcgcgaaccc ggcgaccttc aaggcgctca tcgcggcgcg cggcgccaaa ggaccggtga
     3661 agatgaaggc gacgacccgg aaactgcttt tgcaactcgc cgtccaccat cgctccttcc
     3721 tcgacgaact gcggctcctc catggcggcg ccggcgatgt cgatatcctt gcggggctct
     3781 tcgcccacga tctggccgcc ttcatcgaac tgccgaccgt cgacctcgcg ccgctccatc
     3841 cgccggcgca ggaatggctg acctccgtcg tcggtgacga tgtgtcggcg ctcctcatga
     3901 tgttcggctg gacgctcacg gcgcggcttt cgcagataac gctcgaccgc aacgacggcc
     3961 ccacctcgaa cgagatactg cggaaatacc tgctggaaga ggtctttctg acgcttttcc
     4021 gggaaatggg acgcggcgag aggcacgccg atatggaaca gcggcacatc cgcgcattcg
     4081 tctactatca gaactggtgg cgtcagtatc cacaggacga cctgtgccat gcgatgatga
     4141 aggatgtctt cgccgacgag aacatgcacc gcatcctgca ggtgaacgag tatcgcggca
     4201 tccgctattt caacaaggag gcgttcgagc atttcgtgaa cggcctgttc gtgatggcgg
     4261 tgctcgacat tctccggagc gacctgaccc cggcgaaaaa gaagaaggaa ctcgcaactc
     4321 gcacgacgct ggcacggcgc ttcctcgacg caatgccgct ttctgagtac cgcgtggata
     4381 agttattgga agttattaaa aatcccgtta aggtcccttt gacaagggac cggaaggaag
     4441 aggagggtgg gtggcttatc ggtgaaccgg tgttcgtgcc actcaccgaa cccaagcgcc
     4501 ccgtaaagaa aaagaagacc cccgcaaaaa cgaagaagac caccgccaag aggaaaaaga
     4561 catgagattc atcgcggcgc tcatcgcgct tctgctcgcg gcggccatga cctactacta
     4621 catcaccggt tcgtggcccg gcatgtcgaa agaccccttg aaggacgcct cgaaaaaggc
     4681 cgccatcgag ggcggcccga tcaccgatcc gcagatggcc aaagatcgcg tcgagggaat
     4741 gctgaagaaa agcgaagagg cgcaacgaaa ggcgatagag gactcgaccc aatagcctgt
     4801 gcatgatcct taaaccatgg gagggaacac catgaaacgg ttcatcatca tcggtgtggc
     4861 ggcactgctc gtcctgaccg cctgcgccgg gaccaagacc ggcgatgacc ccgcgccgga
     4921 gaacgacgag aacgcgaccg acacgactgt caacgacacg actgtcaccg atacggcggc
     4981 caccgacacc gccgtaccag atgacaccgt caccgacgaa acgacgaccg acagcgatgt
     5041 cgt
//
LOCUS       DAOQUE010000177         5015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18516_length_5015_cov_4.836492, whole genome shotgun sequence.
ACCESSION   DAOQUE010000177 DAOQUE010000000
VERSION     DAOQUE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18516_length_5015_cov_4.836492"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..308
                     /locus_tag="PKH10_06570"
     CDS             15..308
                     /locus_tag="PKH10_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT27822.1"
                     /translation="MADLTPQERTIVRDILAARLPGVPVHAFGSRVNGTAKPYSDLDI
                     ALVTDTPLPVAQVAELAEAFAVSDLPFRVDIVEWSRIAEDFRAVIAARYEKLQ"
     gene            325..1230
                     /locus_tag="PKH10_06575"
     CDS             325..1230
                     /locus_tag="PKH10_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT27823.1"
                     /translation="MMAPSLPFLGEIAALATACCWVISSLSFQTAGRKVGSLPVNLIR
                     IVLGFLFLSVYLTVVRGTPVPLDATPHAWGWLTLSGVAGFLFGDLFLFKSFVVVGART
                     AMLVMSSVPVLTTVIAYLFMGETIGPIGMIGMAVTIAGIALVVAKRDPAESAARPQWG
                     RGVLYAFGGAFGQAVGLVLSKYGMGAMDPFAATQIRVITGVLGFVALVTYLGAWGRVA
                     AALRDRRSLAFIATGAFFGPFLGVSLSLLSVQYTATGIASTIMSTTPVLIIAPAIVLF
                     KEKVSWREVMGAAVAVGGVALLFWR"
     gene            1310..2479
                     /locus_tag="PKH10_06580"
     CDS             1310..2479
                     /locus_tag="PKH10_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HNT27824.1"
                     /translation="MDQKERLIETFATFTHELMDVVLSNKSFIDINGLSIESPEEAHL
                     YLKNYGYDLDNAVHAEQVRSILNEAKSFIQTYLLEDPEGQTPTLVIPPNVLWEDDPRN
                     FLLMASDRTRSITQRWACAVLRVAHTITHVENDLSKYFFQGIKKQIFARFMTHLNVNN
                     KGEYFIGRGPNQIRLKLFEMKSEKTKESLIMKLLHKKENVTADVFDRVGVRIVTNDKL
                     DVLLVLKYLAENHVISFANIKPSRTRNNLIDLEKFLEGLVRFKDEDLTNWSEEQVREY
                     FKPYLVFDKEKAREQEELIRENNPYSSTQYTSIQLTARQLITIQDPFSINLAYKFFFP
                     FEIQILDHESFVESRTGRASHDEYKKSQIVSVRQRVLENVLRYHARKKWQEVRPV"
     gene            2504..3151
                     /locus_tag="PKH10_06585"
     CDS             2504..3151
                     /locus_tag="PKH10_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNT27825.1"
                     /translation="MRSWMVMFIIALCAATLGGAEKGGIAKKEGTMEKRLNEKKILMI
                     IAPEKFRDEEFAVPHAHFAALGAQVTVASTRKGTATGMFGAKAEATATLAEVKATDYD
                     AVVFVGGAGVPTVRAEPRAVEIAKEAKGLPVLAAICWAPTILAKAGVLAGKKATVWKG
                     DDAEYGMTTDKMLEKNGAKFTGEEVTVDGNIVTGNGPAAAQKFAEAIEKRLSGGK"
     gene            complement(3155..3634)
                     /locus_tag="PKH10_06590"
     CDS             complement(3155..3634)
                     /locus_tag="PKH10_06590"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNT27826.1"
                     /translation="MFTDAVPVGRFARIIKGDGTLLFKPFLRDADFLGTVKHLFHRTF
                     GTEFEITYIREVAKGFHVRIAEVEKPFEAEYLVGEEFTLPRAELLGRSQDALIGTAVV
                     REDGSPVGTVTDLALTPEYPLLTVTGPKKRIELPFTPAVARLEGDRIVLVREDALLN"
     gene            complement(3711..3941)
                     /locus_tag="PKH10_06595"
     CDS             complement(3711..3941)
                     /locus_tag="PKH10_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNT27827.1"
                     /translation="MKELIRHIVSHLVDNPDQIQVDEIDGEQTSVIELRVAKEDLGKV
                     IGKEGRIAKAMRVILSASTSKDRKKSILEIVE"
     gene            complement(3974..4234)
                     /gene="rpsP"
                     /locus_tag="PKH10_06600"
     CDS             complement(3974..4234)
                     /gene="rpsP"
                     /locus_tag="PKH10_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNT27828.1"
                     /translation="MSVSIRLSRLGGKKKPFYRIIAVDKRKKRDGDCLERLGHYDPTR
                     TPVEIVMDVEKINRWIANGAQCSETVASLMKKVAAKAKKAEA"
     gene            complement(4263..>5015)
                     /locus_tag="PKH10_06605"
     CDS             complement(4263..>5015)
                     /locus_tag="PKH10_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019543388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNT27829.1"
                     /translation="VDEAMMREIADIRRLLDPVEVLFVADAMTGQEAVNVSKEFHERV
                     GLTGVVLTKMDGDARGGAALSIHAVTGAPVKFVGVGEKTDQLELFHPERIAGRILGMG
                     DILSLVEKAQEKVDTDKAVKLQKQMLSDRFNLEDFLAQMKMMKQMGPMENLLGMLPGV
                     GGALKQVKGKVDLDRELRRIEAIVQSMTRAERLNPEVLDASRRRRIAAGSGTRVQDIN
                     QFMKQYLEMKKMMKRLKTMGLGSLMKGLKGIG"
BASE COUNT          912 a         1605 c         1524 g          974 t
ORIGIN      
        1 gctcgaatcg cgcaatggct gaccttaccc cgcaggagcg gaccatcgtc cgcgacatac
       61 tcgccgcccg cctgccgggg gtgccggtcc acgccttcgg ttcgcgtgtg aatggtaccg
      121 cgaagcctta ctccgacctc gatatcgcgc ttgtcaccga cacaccgctt ccggtcgcgc
      181 aggtggcgga actggccgaa gccttcgccg tgtcggacct gccgttccgc gtggacatcg
      241 tggagtggtc gcggatagcc gaagatttcc gcgccgtcat cgcggcgcgc tacgagaaat
      301 tgcagtgacc ctcgtgaccg caccatgatg gctccttccc tccctttcct cggcgagata
      361 gcggcactcg cgaccgcctg ctgctgggtg atatcgtcgc tgtcgttcca gacggcgggt
      421 cgcaaggtcg gctcgctgcc ggtcaatctc atccgcatcg tgctcgggtt cctgttcctt
      481 tcggtctatc tgaccgtcgt gcgcggcacg ccggtgcctc ttgacgcgac gccgcatgcg
      541 tggggctggc tcaccctctc cggcgttgcg ggctttcttt tcggcgacct ctttcttttc
      601 aagtccttcg tcgtcgtcgg cgcgcgcacc gcgatgctcg tcatgtcgtc ggtcccggtg
      661 ctcacgacgg tcatcgccta cctgttcatg ggagagacca tcgggccgat cgggatgatc
      721 ggcatggcgg tcacgatagc cggcatcgcg ctcgtggtcg ccaagcggga tcccgcggag
      781 agtgcggcgc gaccgcagtg ggggcgcggg gtgctctatg ccttcggcgg cgcgttcggg
      841 caggcggtgg ggcttgttct ttccaaatac ggcatggggg cgatggatcc cttcgcggcg
      901 acgcagatac gggtcatcac cggcgttctc gggttcgtcg cgctcgtcac ctacctcggc
      961 gcgtggggcc gggtggccgc ggcgctccgc gaccggcggt cgctcgcctt catcgcgacc
     1021 ggcgccttct tcgggccgtt cctcggtgtg tcgctgtcgc tcctgtcggt ccagtacacc
     1081 gcgaccggca tcgcctcgac catcatgtcc accacgccgg tgctcatcat cgcgccggcg
     1141 atagtgctct tcaaggaaaa ggtctcgtgg cgcgaggtca tgggggcggc ggtcgccgtg
     1201 ggcggcgtgg ccctcctttt ctggcgctga cccggccggg gaacgggccc tttttgcatt
     1261 ttgcctttct ctttgctaga atggtcgtcg tccgcaacga ggtggcccga tggatcagaa
     1321 agaacggctc atagagacct tcgcgacctt cacgcacgaa ctgatggatg tcgtgctttc
     1381 caacaagtcc ttcatcgaca tcaacggcct gtccatcgaa tcgcccgagg aggcgcacct
     1441 ctacctcaag aactacggct acgatctcga caacgccgtc cacgccgaac aggtccgctc
     1501 gatactgaac gaggcaaaat cgttcatcca gacctacctg ctcgaggatc ccgagggaca
     1561 gaccccgaca ctcgtcatcc cgcccaatgt cctgtgggag gacgacccgc gcaacttcct
     1621 cctcatggcc tccgacagga cgcggagcat cacccagcgc tgggcctgcg ccgtgctccg
     1681 ggtcgcccac accatcaccc atgtcgagaa cgacctgtcg aaatacttct ttcaagggat
     1741 aaaaaaacag atattcgccc gcttcatgac ccacctcaat gtcaacaaca agggggagta
     1801 tttcatcgga cgcggcccca accagatacg gctgaaactc ttcgagatga agagcgagaa
     1861 gaccaaggaa agcctcatca tgaaactcct gcacaaaaag gagaatgtga cggccgatgt
     1921 gttcgaccgg gtcggtgtcc gcatcgtgac caacgacaaa ctcgatgttc tgctcgtgct
     1981 caagtacctc gccgagaacc atgtcatctc gttcgcgaac atcaagccgt cgcggacccg
     2041 caacaacctg atagacctcg agaagttcct cgagggactc gtccgtttca aggacgagga
     2101 cctcaccaac tggagcgagg agcaggtgcg cgagtacttc aagccctacc tcgtgttcga
     2161 caaggagaag gcgcgggagc aggaggaact catccgtgag aacaaccctt attcctccac
     2221 gcagtacacg agcatccaac tgacggcgcg gcaactcatc acgatacagg atcccttcag
     2281 catcaacctc gcctacaaat tcttcttccc gttcgagata cagatactcg accacgaatc
     2341 gttcgtcgag agccgcaccg gccgcgcgag ccacgacgaa tacaaaaaaa gccagatcgt
     2401 ctcggtgcgc cagcgggtgc tcgagaatgt cctgcgctac catgcccgca agaaatggca
     2461 ggaagtacgg cccgtctaga tagcaaggaa cagggaggat gccatgagat cgtggatggt
     2521 gatgttcatc atcgcgctgt gcgccgcgac gctcggcggc gctgaaaagg gaggaatagc
     2581 gaagaaggag ggcaccatgg aaaaaaggct gaacgaaaaa aagatcctga tgatcatcgc
     2641 gcctgagaaa ttccgcgacg aggagttcgc ggtgccgcac gcccatttcg ccgcgctcgg
     2701 tgcgcaggtg accgtcgcaa gcacgaggaa gggtaccgcc accggcatgt tcggggcgaa
     2761 ggccgaagcg accgccacgc tcgccgaggt gaaggcgacc gattacgacg cggtggtgtt
     2821 cgtcggcggg gcgggcgtgc cgaccgtccg ggccgagccg cgcgccgtcg agatcgcgaa
     2881 ggaggcgaag gggctccccg tcctcgccgc gatatgctgg gcgccgacga tactggccaa
     2941 ggcgggggtg ctcgccggca agaaggcaac ggtgtggaag ggcgatgacg ccgaatacgg
     3001 catgaccacc gacaagatgc tcgagaagaa cggcgcgaag ttcaccggcg aggaggtgac
     3061 ggtcgacggc aacatcgtca ccggcaacgg tccggcggcg gcgcagaaat tcgccgaggc
     3121 gatagagaaa agactgtcag gcggcaaata aaggtcagtt cagaagcgcg tcctcgcgaa
     3181 cgagtactat ccggtccccc tcaaggcgcg ctaccgccgg ggtgaacggc agttcgatcc
     3241 tctttttcgg gccggtcacg gtcaacagcg gatattccgg ggtgagcgcg agatcggtga
     3301 cggtcccgac gggcgatccg tcctcccgca cgaccgccgt cccgatgagc gcatcctgcg
     3361 agcggccgag cagttcggcc cgcgggagcg tgaattcctc gccgacgaga tattcggcct
     3421 cgaacggttt ttcgacctcg gcgatacgga catggaaccc cttcgctact tctctgatat
     3481 aggtgatctc gaactcggtg ccgaaggtgc ggtggaagag gtgcttcacc gtcccgagga
     3541 agtcggcgtc gcgcaggaag ggtttgaaca gcagcgtgcc gtcccccttg atgatgcggg
     3601 cgaaacggcc gacgggcacg gcatccgtga acatagcatc tctcctgctc aaaaaaggcc
     3661 gccagcggcg gcgtgtggcg cgcggccgat accggtcgtc cgccccggac ctactcgacg
     3721 atctcgagta tgctcttttt ccggtccttg ctcgtcgagg cgctcaggat gacgcgcatg
     3781 gccttggcga tgcgcccctc cttcccgatg accttaccga gatcctcctt ggcgacccgc
     3841 agttcgatga ccgaggtctg ttcgccgtcg atctcgtcga cctgtatctg gtcggggttg
     3901 tccaccagat gcgacacgat gtgccggatc agttctttca tgaccttctc ctccctccgt
     3961 agaaaacgat cggttacgct tcggcctttt tcgccttggc ggccactttc ttcatcagcg
     4021 acgcgacggt ctcggagcac tgcgcgccgt tggcgatcca gcggtttatc ttctcgacat
     4081 ccatgacgat ctcgaccggg gtgcgggtcg ggtcgtagtg gccgagccgt tcgagacagt
     4141 ctccgtcccg tttcttgcgt ttgtcgacgg cgatgatgcg gtaaaagggt ttcttcttgc
     4201 cgcccagccg ggaaagtctg atgctgacgc tcatgcgaat gttcctccta tgcgcatggt
     4261 tgctatccga tgcccttgag acccttcatc agcgagccga gccccatggt cttcaatcgt
     4321 ttcatcatct tcttcatctc gaggtactgt ttcatgaact ggttgatgtc ctgcacccgc
     4381 gtgccgctcc cggccgcgat acggcggcgg cgcgacgcgt cgagcacctc ggggttcagc
     4441 cgttcggcgc gggtcatcga ctggacgatc gcctcgatgc ggcgcagttc gcggtcgaga
     4501 tcgaccttgc ccttcacctg tttgagcgca ccgccgacgc ccggcaacat gccgagcagg
     4561 ttctccatcg ggcccatctg cttcatcatc ttcatctggg cgaggaaatc ctcgaggttg
     4621 aaccggtcgg agagcatctg cttctgcagt ttcaccgcct tgtcggtatc caccttctcc
     4681 tgcgccttct cgacgagcga gagtatgtcg cccatgccga gtatgcgccc cgcgatccgt
     4741 tcggggtgga acagttccag ttgatcggtc ttctcgccca cgccgacgaa cttgaccggc
     4801 gcgccggtca ccgcgtgtat cgagagcgcc gcgccgccgc gcgcgtcgcc gtccatcttg
     4861 gtcaggacga cgccggtgag cccgacgcgc tcgtggaact ccttcgacac attgaccgct
     4921 tcctgtcccg tcatcgcgtc ggcgacgaac agcacctcga cggggtcgag cagccgtcgg
     4981 atatcggcga tctcgcgcat catcgcctcg tcgac
//
LOCUS       DAOQUE010000178         5012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18535_length_5012_cov_3.468832, whole genome shotgun sequence.
ACCESSION   DAOQUE010000178 DAOQUE010000000
VERSION     DAOQUE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18535_length_5012_cov_3.468832"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PKH10_06610"
     CDS             complement(<1..393)
                     /locus_tag="PKH10_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT27830.1"
                     /translation="MHILFLHGFAAGGAVWEPYRSEFPDARMPTLQCSGDGEPILPEL
                     DEPAILVGWSMGAMLAVELLRRQPQFVKGLVLVSSSPSFVVSDVFPEGKAPSAVRELR
                     DAITKGDPKGLQGFQKQLFTAAEIRDGWL"
     gene            577..3114
                     /locus_tag="PKH10_06615"
     CDS             577..3114
                     /locus_tag="PKH10_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27831.1"
                     /translation="MHRITVLIMVLGLTASLFAAAPSAIKKAQVRYDRGTPVMVTGLQ
                     VPLKADAATVEKFVKNEFFPAFKLGKDISLVPFRTVTIDGTAIFKYGHAYKGWPVEGA
                     YTTVSVKNGKAYRVNNGLGAIDLDLSNLVSPDKAVRAAMAKRFAKQPAALPKHLAEKV
                     IINIGGAYLPAYRFSFMPSSLADNRYYYVNAKTGAFIKMHDRVMYADDDTLLSDDPMT
                     ALDLAKVFTKNPISTPDLVEVELPWVAPADDADLAAEARGFLTAKKDSSDIRKIKAFN
                     CPNKDEKLNLEPIVGYAIMLPICTPTQLANKVSNGSFIYDDCKGDQTFVEANMREDKI
                     DKCAEISMYYHASKIYQYIRQLDTGFEYLSGNKADAPLNVIGNFQMVDMNDLGAIIGG
                     TAKLAAMDNAFFSPDNPMIAQFFETYGIKGDLLVFGQGSFADFGLDGDVVYHEFGHAT
                     VYTTGLDSGAFLDKYGMNGEPGALHEGFGDTFSFIISDDPCTGEYASDGIVKWANSQG
                     GFVEMDKYDDGEKEYWCMRYAEHEYKVIEDWEGEVHWDGQPHLAANWAIYKLAQKDQI
                     GGTTLEEQKAAFTKLLLKTLYALGTSIATHELWADTLLSEIDNDDAYKTHKAHIEQIL
                     TDFNYFTEIRARDGSKTIERLFQDAAVDPEGGSSPLGGGGTSIQITEGEEQIPIAPGY
                     LQIYYTVPEDFEFSALRVTATVAASGGSDPMGGTSEPDLHVWARKTDPIEYTIESSSS
                     SVVLVSAKYDADIEKDTAKNGWYLTGVEPGEKYYLQFVNFGFGGATVTSIAIAGENYV
                     PATDEDVIGADEDTIAATDEEEVDTVVVPDTGTVTKKDDGCGCSLVF"
     gene            3269..4318
                     /locus_tag="PKH10_06620"
     CDS             3269..4318
                     /locus_tag="PKH10_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27832.1"
                     /translation="MHPARWVILFGLMLFFAAACDEDGLAGKNSLVKLSEEPAGGACP
                     QGGTKVESGLDLDGDGELDLDEVRNVQYFCRDAGCTITDNGDGTKTIACDGDSSVTVK
                     DGADGAGCAMTDNEDGTYTITCDGVSVIVSDASGDGAHKTLIVLEEAPGDTCPNGGQK
                     VTVGIDRDDDGTLDTDEVTSTRYLCNGADGHDSLTTIDPLTPGEDCPAGGHLIRNGVD
                     TDDDGTLDAGEVVSEVAVCNGADGNGAMTEVSPLDPGEGGCAAGGYLFRTGVDANGNG
                     ALDTGEYDEVAVCHGEKGDPGEDGVCAGNHPPLIDAYDDRRVVAVCHGEKGDPGEDGV
                     CAGNHPPVIVSIDVP"
     gene            4367..>5012
                     /locus_tag="PKH10_06625"
     CDS             4367..>5012
                     /locus_tag="PKH10_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27833.1"
                     /translation="MTVNADDEDGDALSYEAIGGYASIAAGAEPNQIDVTPRALGGPF
                     SFAVIVSDGCQVAMGDFVIGEVWDETTPPFIDNSDIWFEDITGTSVTVRWEKAWDDWS
                     AIQDLEYKVVYALDPDEIDDIDLIELGGGTIAMDWTVDVDAAPIAGLTEGQQYYATVL
                     VRDEYHNMSQYITAPFTMPDVTAPTTGGDISFSDITETSFTVSWDSASDNLSDLS"
BASE COUNT         1054 a         1610 c         1538 g          810 t
ORIGIN      
        1 gagccacccg tcgcgtatct cggcggcggt gaagagttgt ttctggaaac cctgaagccc
       61 cttcggatcg cctttcgtta tcgcatcgcg cagttcgcgg acggcggacg gcgccttgcc
      121 ttcggggaag acatcgctca cgacgaacga cggcgacgac gagacgagca caagcccttt
      181 gacgaactgc ggttgacgcc gcaacagctc gaccgcgagc atcgccccca tcgaccagcc
      241 gacgagtatg gcgggttcgt cgagttcggg aagtatcggt tcgccatcgc cggagcattg
      301 aagcgtcggc atacgggcgt cggggaattc ggaacggtac ggctcccaga ccgcgccgcc
      361 cgccgcaaaa ccgtggagga acaggatgtg cataccatac agtccgcttt tgaccagtgg
      421 cgagtatagc acattccgcg acgagggcaa ctaattaaga tcagggcggt cgcgcacgtc
      481 catttaaaaa agagaggtag ccgttgactt tgtctccgac gccgctatat aatcgccgga
      541 atgggaaagt gtccaccaac gactaggagg tcgaacatgc acagaatcac cgttctcatc
      601 atggtcctcg ggctgacggc gtcgcttttc gccgccgcgc cgagcgcgat caaaaaggcg
      661 caggtccgct acgaccgcgg cacgccggtg atggtcaccg gtctgcaggt cccgctcaag
      721 gccgatgccg cgacggtcga gaagttcgtg aagaacgaat tcttccccgc cttcaaactg
      781 gggaaggaca tctcgctcgt cccgttccgc accgtcacga tcgacggcac cgccatcttc
      841 aagtacggtc acgcctacaa gggctggccg gtcgaaggcg cctacaccac cgtttcggtc
      901 aagaacggca aggcctaccg ggtcaacaac gggctcgggg ccatcgatct cgacctgtcg
      961 aacctcgtgt cgcccgacaa ggcggtccgc gccgcgatgg cgaaacggtt cgcgaaacag
     1021 cccgccgccc tgcccaagca cctcgccgaa aaggtcatca tcaacatcgg cggcgcctac
     1081 ctgccggcct accgcttctc cttcatgccc tcttcgctcg ccgacaaccg gtattactat
     1141 gtcaacgcga agaccggcgc tttcatcaag atgcacgatc gcgtgatgta cgccgacgac
     1201 gacacgctgc tgtcggacga cccgatgacg gcgctcgatc tcgcgaaggt cttcacgaag
     1261 aacccgatat cgacccccga cctcgtcgag gtcgaactgc cgtgggtcgc cccggccgac
     1321 gacgccgatc tcgccgccga ggcgcgcggg ttcctcaccg cgaagaagga cagcagcgac
     1381 atccgcaaga tcaaggcgtt caactgtccc aacaaagacg agaaactgaa tctcgagccg
     1441 atcgtcgggt acgcgatcat gctgccgatc tgcaccccga cccaactcgc gaacaaggtc
     1501 tccaacggca gtttcatcta tgatgactgc aagggcgacc agaccttcgt cgaggcgaac
     1561 atgcgcgagg acaagataga caagtgcgcc gagatatcta tgtactacca cgcgtcgaag
     1621 atctatcagt atatccgcca actcgacacc ggtttcgagt acctctcggg caacaaggcc
     1681 gacgcgccgc tcaatgtcat cggcaacttc cagatggtcg acatgaacga cctcggcgcc
     1741 atcatcggcg gcaccgcgaa actcgccgcg atggataacg ccttcttcag ccccgacaac
     1801 ccgatgatcg cccagttctt cgagacctac ggcatcaagg gcgacctgct cgtcttcggg
     1861 caggggtcct tcgccgactt cggtctggac ggcgatgtgg tctatcacga gttcggccac
     1921 gccacggtct acaccaccgg tctcgactcg ggcgctttcc tcgataaata cggcatgaac
     1981 ggagaaccgg gcgcgctcca tgagggcttc ggcgacacct tctcgttcat catatcggac
     2041 gacccgtgca ccggcgaata cgcctccgac ggcatcgtga aatgggccaa ctcgcagggc
     2101 ggcttcgtcg aaatggacaa atacgacgac ggcgagaagg aatactggtg catgcgctac
     2161 gccgagcacg agtacaaggt catcgaggat tgggagggcg aggtccactg ggacggtcag
     2221 ccgcaccttg cggccaactg ggccatctac aaactggcgc agaaggacca gataggcggc
     2281 acgacccttg aggaacagaa ggcggccttc acgaaactgc tcctgaagac gctctacgcg
     2341 ctcggcacct cgatagccac ccatgaactc tgggccgaca cccttctttc cgagatcgac
     2401 aatgacgacg cctacaagac gcacaaggcc catatcgagc agatattgac cgacttcaat
     2461 tacttcaccg agatccgcgc ccgcgacggc tcaaagacga tagagcgcct cttccaggac
     2521 gcggcggtcg atccggaggg cgggtcaagc ccgctcggcg gcggcggcac ctcgatccag
     2581 ataaccgagg gcgaggaaca gataccgata gcccccggct acctccagat atactacacc
     2641 gtgcccgagg acttcgaatt ctcggcgctc cgcgtgaccg ccaccgtggc cgcgagcggc
     2701 ggctccgacc cgatgggcgg caccagcgaa ccggacctgc atgtgtgggc gcgcaagacc
     2761 gacccgatcg agtacacgat agaaagttcc tcgtcgagcg tcgtgctcgt ttcggccaaa
     2821 tacgacgccg acatcgagaa ggacaccgcg aagaacgggt ggtacctcac cggcgtggaa
     2881 ccgggcgaga agtactacct gcagttcgtg aatttcggct tcggcggcgc gacggtgacg
     2941 agcatcgcca tcgccggcga gaactatgtc ccggcgaccg atgaggatgt catcggcgct
     3001 gacgaggaca ccatcgccgc gaccgacgag gaagaggtcg acacggtcgt cgtccccgac
     3061 accggcacgg tgaccaagaa ggacgacggt tgcggttgct cgctcgtatt ctgatcgatc
     3121 ggcatcctgt tcccatttcc acaggacggc acggggttcc cgtgccgtcc tttttttatc
     3181 cggtgaaaat ttgtgttttt ccgacgggat aatagaatcg tcccgtcgtt cgtcacgaag
     3241 tcacattcac tgaggagagg aggttatcat gcatcctgca cgctgggtga tcctgttcgg
     3301 cctgatgctt tttttcgccg ccgcctgcga tgaggacggt ctcgcgggga agaactcgct
     3361 cgtcaaactg agcgaagagc cggccggcgg ggcctgtccg cagggcggca ccaaggtcga
     3421 gagcggtctc gatctcgacg gtgacgggga actcgacctc gacgaggtca ggaatgtcca
     3481 gtatttctgc cgtgacgcgg gatgcaccat caccgacaac ggcgatggga ccaagacgat
     3541 cgcctgcgac ggggacagca gcgtcaccgt gaaggacggc gccgacggcg ctggctgcgc
     3601 catgaccgac aacgaggacg gtacctacac catcacctgc gacggcgtgt cggtgatcgt
     3661 cagcgacgct tcgggcgacg gcgcccacaa gacccttatc gtgctcgaag aggcacccgg
     3721 ggacacctgt ccgaacggcg gccagaaggt gaccgtcggc atcgaccgcg acgatgacgg
     3781 tacgctcgat accgacgagg tgacctccac gcgctacctc tgcaacggcg ccgacggcca
     3841 cgactcgctg accacgatag atccgctgac cccgggcgag gactgccccg ccggcggaca
     3901 cctgatacgc aacggcgtcg acaccgacga cgacggcacg ctcgatgcgg gcgaggtcgt
     3961 gagcgaggtg gcggtctgca acggagccga cggcaacggc gcgatgaccg aggtctcccc
     4021 gctcgacccg ggcgagggcg gttgcgcggc gggcggctac ctgttccgga ccggcgtcga
     4081 cgcgaacggc aacggtgcgc tcgatacggg cgaatacgac gaggtggcgg tctgtcacgg
     4141 cgagaagggc gacccgggcg aggacggcgt ctgcgccggc aaccacccgc cgctcatcga
     4201 tgcttacgat gaccggcggg tggtggcggt ctgtcacggc gagaagggcg acccgggcga
     4261 ggacggcgtc tgcgccggca accacccgcc ggtcatcgta agcatcgatg tcccgtagcc
     4321 agacggtcta caggaagaca cctaccggct cgacatacca tacatcgtga cggtgaacgc
     4381 cgacgatgag gacggcgacg ccctctccta tgaggcgatc ggcggctacg catccatcgc
     4441 cgcgggcgcg gaaccgaacc agatcgacgt gacgccgcgc gcgctcggcg gccccttctc
     4501 gttcgccgtg atcgtcagcg acgggtgtca ggtggctatg ggcgacttcg tcatcggcga
     4561 ggtgtgggac gagacgaccc cgcccttcat cgacaacagc gatatctggt tcgaggacat
     4621 caccggcacg agcgtcaccg tccgctggga aaaggcatgg gacgactgga gcgcgataca
     4681 ggacctcgaa tacaaggtgg tctatgcgct cgacccggac gagatagacg atatcgacct
     4741 tatagaactg ggcggcggca cgatcgccat ggactggacc gtcgatgtcg atgccgcgcc
     4801 gatagcggga ctcacggaag gccagcagta ctacgcgacg gtgctggtcc gcgacgagta
     4861 ccacaacatg agccagtaca tcaccgcccc cttcacgatg cccgatgtga ccgcgccgac
     4921 caccggcggc gacatctcgt tcagcgatat caccgagacc tcgttcaccg tctcgtggga
     4981 cagtgcgagc gacaatctca gcgacctgtc gg
//
LOCUS       DAOQUE010000179         4998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18610_length_4998_cov_4.337042, whole genome shotgun sequence.
ACCESSION   DAOQUE010000179 DAOQUE010000000
VERSION     DAOQUE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18610_length_4998_cov_4.337042"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PKH10_06630"
     CDS             complement(<1..423)
                     /locus_tag="PKH10_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNT27834.1"
                     /translation="MARKKSVTQKKAPDAKQLVEKLCHKKRSGWDGISVAEEREIMEF
                     AEDYKRFLDDSKTEREAVVTIGRIARRGGYRPIGEATKKDSRLFIDFHGVSCVLAQIA
                     DPKKFAEGFLMVGAHIDTPRLDLKGAPLYEAEDMAYLKT"
     gene            616..1200
                     /locus_tag="PKH10_06635"
     CDS             616..1200
                     /locus_tag="PKH10_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27835.1"
                     /translation="MTYLPLSYLLGSRAKVDILRILVSKQNYTGRKIATLSSTNPNSN
                     KKALDFLLGTGLVSREHIGNAYHYRLNENHILHDAVLNLFKEEERTISRIMKTAGSYV
                     EEYAPKTLAGFVTYLRSDVVLTIIAHSLSVPEMKEYIAEKTGFNVQIKVIDTLNLSES
                     DVVEFVDRNYFWNNYKTILKMIHSRDIVKDFFNI"
     gene            1210..1626
                     /locus_tag="PKH10_06640"
     CDS             1210..1626
                     /locus_tag="PKH10_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT27836.1"
                     /translation="MNKKNIRILVLDDEENILSSLKRAIEARGYTVIVKSKPEEAIDY
                     IRNNIVHVALLDILMPNMDGITVLNIVKEISGLTQVIMMSAYSTIDRIVSALESGAND
                     FLLKPFDNIDELISVIDLSVQKLDRWKKVLSESGAI"
     gene            1629..2909
                     /locus_tag="PKH10_06645"
     CDS             1629..2909
                     /locus_tag="PKH10_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNT27837.1"
                     /translation="MTRHPSSRARLTLISLHDLSLPPTFAADFVTALIGAFEREKGQA
                     IGHSTRVTLLSHLIGQEMRLPRETQKELFFSSLLHDIGGISTAGHIWKRLTEVPDIFG
                     QKTDFRIFFHSARGYAVLSRFPTLRKIAEVVYTHHEFYDGSGFPQGLKGDDIPLLARI
                     IRIADTTDIILRLHEIQNGEEIKSFLDISAGEEFDPQSYERFVSLLGRPDTLPLLLDE
                     NLVRERFREICQSMTDQYYFSASDSLNRFFEMAAMLIDNVTTIGKQHSLRVAEFAEQI
                     ALSLKLDQSLILQMRWAALLHDIGKVTSNRMIYTKSEKLSDIEWRAIRAHPEKGYETL
                     NAVSGLQEIAFYVLHHHENYDGSGYPEGLSGEKIPLASRILRVADAFEAMTSERIYHR
                     KRDWQMALRELRENAGKQFDPRIVDITVDAYQIR"
     gene            2890..4377
                     /locus_tag="PKH10_06650"
     CDS             2890..4377
                     /locus_tag="PKH10_06650"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNT27838.1"
                     /translation="MRTRYVEAYMKVVLAGYNVDAEILRELKEKSRWDADNVTPETLS
                     AAYARISRDPRDIEELRADSRTATDKARRSNETIIFGLGHASVAEHAVFNLDIIGVSR
                     LAAEAVQQFRLAAYTEKSQRYITLDGDFVTPPGITAAGLEKKFRAAVAFQNDTYRELY
                     AALKTRLFKKHADLLATKNGERTVDGWAKEDARYVVGLATATQFGMTVNARELEHVLR
                     RLRADRRGEIRTLAEALHAPLAALSPSIIKYARPTGYDTDRVEELALLAQRTLSQATG
                     SSGELVRLVAHTDDPDLALCAALLFRYGGVAYDAARAAAGAMSAADRRELVRTALSQR
                     SCWDRVDRAFEAMEFSFELIVSATNYAQLKRHRMTTQLVQDYEPALGVTVPPNIIEIG
                     MEKEFQAAIARGEECYATLAGKIPEERDYILTNAHRRRVLIKLNARELYHFAALREDE
                     HAQWDIRETARLMREAAERVAPLTMMMLCGKSDFDARRTEILGTE"
     gene            4383..4721
                     /locus_tag="PKH10_06655"
     CDS             4383..4721
                     /locus_tag="PKH10_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor protein"
                     /protein_id="HNT27839.1"
                     /translation="MLPDWAKEFGASITVCDENGILLYLNEKARSIFETPENAPLVGK
                     SMFDCHKQASQEMIRAMMATKKPHVYTIEKKGVKKLIYQAPWFENGVFKGLVELSMEL
                     PAEVPPHRVR"
     gene            complement(4718..>4998)
                     /locus_tag="PKH10_06660"
     CDS             complement(4718..>4998)
                     /locus_tag="PKH10_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27840.1"
                     /translation="PFVADGSRSYYCADCLKEFNAEKKRGTVKQVTDPKGRRRYEYIC
                     DVCLRLRRAGTPPRKDRDRLICGECAEKEARERKASGRKRVVIAKKGA"
BASE COUNT         1064 a         1573 c         1407 g          954 t
ORIGIN      
        1 ggtcttgaga taggccatgt cctccgcctc gtagagcggc gcacccttga gatcgagccg
       61 cggcgtgtcg atgtgggcgc cgaccatcag gaacccttcg gcgaactttt tcggatcggc
      121 tatctgggcg agcacgcagg agaccccgtg gaaatcgatg aagagccggg agtccttctt
      181 ggtcgcctcg ccgatgggac ggtagccgcc gcgacgggcg atacgaccga tggtgacgac
      241 cgcctcgcgc tcggtcttgc tgtcatcgag gaaccgcttg tagtcctcgg cgaactccat
      301 tatttcgcgc tcctcggcga cagagatgcc gtcccagccc gaccgtttct tatgacacag
      361 tttttcgacc agttgcttgg catcgggcgc ttttttctgg gtgacgcttt tcttgcgggc
      421 catcgcggtc ctcctcccgg ttcaccggtg ataaaagtgg ttacggaacc cgatactagt
      481 gcaaagtgcg gcgaaacaca aacttttccg gaaccggctc tgggaagagt tgcgtttttt
      541 gtataatttt agtggacttt ctgtatcttt gtgttatatt cgttttactt ttattgtccg
      601 atcgtggagg gctagatgac ctatctgccg ctttcgtatc ttctcgggtc ccgcgcgaag
      661 gtggatatcc tgaggatcct cgtgagcaag cagaactaca ccggtcgcaa gatcgcgacg
      721 ctttcgagca ccaacccgaa ctcgaacaag aaggctctcg acttcctgct cggtacgggg
      781 ctcgtgagcc gcgagcatat cgggaatgct tatcactacc ggctcaacga gaaccacatc
      841 ttgcacgatg cggtactcaa ccttttcaaa gaggaggaac ggaccatcag ccgcatcatg
      901 aagaccgccg gatcctatgt cgaagaatac gcccccaaga cgctggccgg cttcgtgacc
      961 tacctgcgaa gcgatgtggt gctcacgatc atcgcccact ccctttcggt gccggagatg
     1021 aaggaataca tcgccgaaaa gaccggattc aatgtgcaga taaaggtgat agacaccctc
     1081 aatctttccg aaagcgatgt cgtcgagttc gtggatcgca actacttctg gaacaactac
     1141 aaaacgatcc tcaagatgat acactcgcgc gacatcgtaa aagatttctt caacatatag
     1201 ggggcgacga tgaacaagaa gaatatccgc atactggtcc tcgacgacga ggagaacatc
     1261 ctctcctcgc tcaagcgcgc catcgaggcg cgcggttaca cggtcatcgt caaatcgaag
     1321 ccggaggagg cgatcgacta tatccgcaat aacatcgtgc atgtcgcgct cctcgacatc
     1381 ctcatgccca acatggacgg catcacggtg ctcaatatcg tcaaggagat cagcggactc
     1441 acgcaggtca tcatgatgag cgcctacagc acgatagacc gcatcgtttc ggcacttgaa
     1501 agcggcgcga acgatttcct gctcaaacca ttcgacaata tcgacgaact catctcggtc
     1561 atcgacctgt cggtgcagaa actcgatcgc tggaaaaagg tgctttccga gtccggagcc
     1621 atctgaccgt gacccgtcac ccctcctctc gcgcacggct caccctcata tcgctccacg
     1681 atctcagtct gccgcccact ttcgccgccg atttcgtcac ggcgctcatc ggcgccttcg
     1741 agcgcgaaaa ggggcaggcc atcgggcata gtacgcgggt cactcttctt tcccatctga
     1801 tagggcagga gatgcgcctg ccacgggaga cccagaagga gcttttcttc tcctcgctac
     1861 tgcacgatat cggcggcatc tccaccgccg ggcacatctg gaaacggctc accgaggtcc
     1921 ccgacatctt cggtcagaag accgatttcc gcatattctt ccattcggcg cgcgggtatg
     1981 ccgtcctgag ccgattcccc actctccgca agatagcgga ggtggtctac acccatcacg
     2041 agttctacga tggttcgggc ttcccgcagg gactcaaggg cgacgacatc ccgctccttg
     2101 cccgcatcat acggatcgcc gacaccaccg acatcatcct gcgtctccat gagatacaga
     2161 acggagagga gataaagagt ttcctcgaca tcagcgccgg agaagagttc gaccctcagt
     2221 cttacgagcg gttcgtctcc ctgctcgggc gaccggacac gctgccgctt ctgctcgacg
     2281 agaacctcgt gcgtgaacgg ttccgggaga tatgccagtc gatgaccgac cagtactatt
     2341 tctcggcctc cgattcactt aaccggttct tcgagatggc ggcgatgctc atcgacaatg
     2401 tgacgaccat cggcaaacag cattccctgc gggtcgccga attcgccgag cagatcgccc
     2461 tgtcgctcaa acttgaccag agcctgatcc tccagatgcg ctgggcggcg ctcctgcacg
     2521 acatcggcaa ggtgacaagc aaccgcatga tctacaccaa gtcggagaaa ctttccgaca
     2581 tcgagtggcg cgccatccgc gcccaccccg aaaagggcta cgaaacgctc aacgccgttt
     2641 cgggtctgca ggagatagcg ttctatgtcc tgcatcacca cgagaactac gacggttccg
     2701 gctatcccga gggactgtcg ggagaaaaga taccgctcgc ctcgcgcatc ctgcgggtcg
     2761 ccgacgcgtt cgaggcgatg acctccgaac ggatatacca ccgcaagcgc gactggcaga
     2821 tggcgctccg cgaactgcgc gagaacgccg gcaagcagtt cgacccccgc atcgtcgata
     2881 tcaccgtcga tgcgtaccag atacgttgag gcatacatga aggtcgttct cgccggctac
     2941 aatgtcgatg ccgagatact gcgcgaactg aaggaaaaat cgcgctggga cgccgacaat
     3001 gtgacgcccg aaacgctttc ggcggcctac gcccgcatat cgcgcgatcc ccgcgacatc
     3061 gaggaactgc gtgccgattc gcgcaccgcg accgacaagg cgcgcaggtc gaacgagacc
     3121 atcatcttcg gacttggcca cgcgtcggtc gccgaacacg ccgtgttcaa cctcgacatc
     3181 atcggcgtct cgcggctcgc cgccgaggcg gtccagcagt tccggctcgc cgcctatacc
     3241 gagaaatcgc agagatacat caccctcgac ggcgacttcg tgacaccgcc cggcatcacg
     3301 gcggctgggc ttgaaaaaaa gttccgcgcg gcggtcgcct ttcagaacga cacctatcgc
     3361 gaactctacg cggcgctcaa gacccgtctt ttcaaaaagc acgccgatct cctcgcgacg
     3421 aagaacggcg aacggacggt cgacggctgg gcgaaagagg atgcccgcta tgtggtgggt
     3481 ctcgccaccg cgacgcagtt cggcatgacc gtgaacgccc gggaactgga gcatgtcctg
     3541 cgccgcctcc gcgcagaccg gcgaggcgag atacgcacgt tggccgaagc gctccatgcc
     3601 ccgcttgcgg cgctgtcgcc ttccatcatc aagtacgcga ggccgaccgg ctacgacacc
     3661 gaccgcgtcg aagaactggc ccttcttgcg cagagaaccc tgtctcaagc gaccggaagt
     3721 tcaggtgaac tcgtgcggct cgtggcgcac accgacgatc cggatctcgc cctctgcgcg
     3781 gccttgctgt tccgctacgg cggcgtcgcc tacgacgcgg cccgcgcggc ggcgggcgcg
     3841 atgagcgccg cggatcgacg ggaactcgtc cggacggcgc tctctcagcg ctcctgctgg
     3901 gaccgtgtcg accgcgcctt cgaggcgatg gagttctcct tcgaattgat cgtgagcgcc
     3961 accaactacg cgcaactcaa acgccaccgg atgacgacac aactcgtgca ggactatgag
     4021 cccgcgcttg gtgtcaccgt gccgcccaac atcatcgaga taggaatgga aaaggaattc
     4081 caggcggcga tcgcccgcgg cgaggaatgt tacgcgacgc tcgccgggaa gatacccgaa
     4141 gagagggact acatactcac caatgcccac cggcgccggg tgctcatcaa actcaacgcc
     4201 cgtgaactat accacttcgc cgccctgcgc gaggacgaac acgcccagtg ggatatccgc
     4261 gaaacggcgc gtctcatgcg cgaagcggcc gagagggtcg cgcccctcac gatgatgatg
     4321 ctgtgcggca agagcgactt cgacgcacgg cgcaccgaga tattaggaac ggagtaacga
     4381 ccatgcttcc tgattgggca aaggaattcg gcgcctcgat caccgtctgc gacgagaacg
     4441 ggatactgct ctatctcaac gaaaaggcgc ggagcatctt cgaaacgccg gagaacgcgc
     4501 cgcttgtcgg caagagcatg ttcgactgtc acaaacaggc atcgcaggag atgatccggg
     4561 cgatgatggc gaccaagaag ccccatgtct acacgataga gaagaagggc gtcaaaaaac
     4621 tcatctatca ggcgccgtgg ttcgagaacg gcgtcttcaa aggactcgtt gaattatcga
     4681 tggaactgcc ggccgaagtg ccgccgcacc gggtccgcta ggcgcctttt ttcgctatca
     4741 ccacccgttt gcgaccgctc gccttccgtt cgcgcgcctc tttctcggcg cattcgccgc
     4801 agatcaaccg gtcgcggtcc tttctcggcg gggtccccgc tcgccggagc cggaggcaga
     4861 catcacagat gtattcatag cgccgacgtc ccttgggatc ggtcacctgc ttgacggtgc
     4921 cgcgcttctt ctcggcgttg aattccttga ggcagtcggc gcagtagtaa ctgcgcgagc
     4981 cgtcggcgac gaagggga
//
LOCUS       DAOQUE010000180         4977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18724_length_4977_cov_4.739130, whole genome shotgun sequence.
ACCESSION   DAOQUE010000180 DAOQUE010000000
VERSION     DAOQUE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18724_length_4977_cov_4.739130"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /locus_tag="PKH10_06665"
     CDS             <1..1828
                     /locus_tag="PKH10_06665"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT27841.1"
                     /translation="GMQIESDTPEVWDMRKTALNLMLSEHSGDCEAPCTIACPAHAQV
                     EEYVREGKKGNLLEALKLIKERIPLPMSIGRVCPRFCEKECRRNVVDKEPVAINDFKR
                     LAADMHYDSYMEEMKPFTGKKVAIVGAGPAGLSVAYFLRLNGVASDLYDKMPQPGGML
                     RYGIPEYRLPKGILDKEIAHFAKMGGIAIHCNKELGKDLAIEDLQKKYDAVAITVGSW
                     KSSAAGVEGEELAVGGIQYLEKIALAGWKGDNPGKTIVIGGGNTAMDCLRTSVRLGSK
                     EVTCFYRRTEAEMPAEKIEIHEAKEEGVKFEFLVAPVKLRKENGRLVLTSQRMTLGEP
                     DASGRRKPVPVPNSDFDTVADTVIAAIGQKTIAPKGVKANKWGDVEVSKENCRMEGSI
                     YAAGDCVSGPATVVEAVAGARRTALAILAQIESKEFKEPYLLNVSRGKWQSLKTDDLV
                     FLKDPIERKRTPQRLIPEHDRVTTFKEVSLTYTQAEITKEGERCIECSCTAKGDCSLK
                     KHSETYTAKPDAMVGEKPNFGYDVRHPEIILDRGKCIKCGVCVKVCGEVVNQNLLGFK
                     FRGFGTHVDTAFSQPLPSQLCRDCGKCIGECPVGALDWKLKK"
     gene            1912..2373
                     /locus_tag="PKH10_06670"
     CDS             1912..2373
                     /locus_tag="PKH10_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT27842.1"
                     /translation="MTRENKTAKYDRIVAQLEELYLKTPDPIARMATASALLKGKFKY
                     FFWVGFYRLVGGELTVGPYQGALACQVLKKHTGVCWAAIDRGETVLVPDVEAFPGHIA
                     CDSRSRSEVVVPLRDRDGRIWAVLDVDSDELDSFDADDVAGLKKVAALIDP"
     gene            complement(2407..4317)
                     /locus_tag="PKH10_06675"
     CDS             complement(2407..4317)
                     /locus_tag="PKH10_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNT27843.1"
                     /translation="MISPHHSSFKMAFFILPLLWLSACSYDEPEGVECVTAFDCGDGL
                     VCEDGFCVESPDQSGGTDTETIVTDKATDEEAEAMPEADVSDEAIEQDAPIDDDQFND
                     TSSDPDEDEIPQDTLASDNDDIDGTGPLGDEDIEAGTEDDVTIKDDALPLDDTLPFDD
                     GPLPDIDQNSDTTPPTVTLTSPAANETGVGIATTVAATFSEPMNALTLTPTTMTVKQD
                     ATPVNGAVSYDSGSMTATFTPASVLAYDTTYTVTVTTGAQDEAGNGLTVDHVFSFTTA
                     VEEINECAPDGKGPCDDNGDAAATCNDTLGGYECSCSWGFEFLTGSCRNIDECTEVSH
                     DCPSNAHCADATPGFTCICNDYYTGGGYDCTFCNTAGQCGAACAACGSGAPYCRDYGN
                     GTSACVQCLEEGHCGDGTPHCLTSNNTCVQCRDNNDCNTGAGEVCSIVTHTCAANVCG
                     DGVPGGLIDAFTETFEGGLPNYSNGDWDTSSTQKHTGSYSLKSGSVPDDGGTSSVSML
                     RYTDGQICFWYAGQSESFDKFRVYINGSKLFETSGDKRTWSEQCLPTTAGFQTLEFEY
                     YKDTSNYVGWDAFYVDDIRFHEATTEQCEAGGQTMNCDAINKCGTVPCNNTCDGFSFD
                     SCSDPDQWGSCS"
     gene            complement(4350..>4977)
                     /locus_tag="PKH10_06680"
     CDS             complement(4350..>4977)
                     /locus_tag="PKH10_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27844.1"
                     /translation="DDTVTDDTVTDDTVTDDTMTDDTVTDDTATDDAVDIEAVDEQPD
                     EDTAVTCESVTMGFDGTWTDPRGNNWTLSCTGTGCINWIYSHAIYDNRAAHEPPTNAE
                     TSTAYLVSPEYHRKSGCSLTITYDYRFQENATTADSLKLEVCQGSCSSWTTVKTWTSF
                     TSSIYGESTDLSAHVTPGSAFKVRWAHYSPKVTTHYTAWVDNFEMDGI"
BASE COUNT          977 a         1452 c         1539 g         1009 t
ORIGIN      
        1 cgggatgcaa atcgagtccg ataccccgga agtgtgggat atgcgcaaaa cggcgctcaa
       61 cttgatgctt tccgagcaca gcggcgactg cgaagcgccc tgcaccatcg cctgtccggc
      121 ccatgcgcag gtcgaggagt atgtccgcga aggaaagaaa ggcaacctgc tcgaggcgct
      181 caaactcatc aaggagcgga tcccgctccc gatgtcgatc ggtcgcgtct gtccgcggtt
      241 ctgcgaaaag gagtgccgcc gcaatgtcgt cgacaaggaa ccggtcgcca tcaacgactt
      301 caaacggctc gccgccgaca tgcattatga ttcctatatg gaagagatga agccgttcac
      361 cggcaagaag gtcgccatcg tcggcgccgg tcccgccggt ctgtcggtcg cctatttcct
      421 gcggctgaac ggcgtcgcgt cggatctcta cgataaaatg ccccagccgg gcggcatgtt
      481 gcgctacggt atccccgaat atcgcctgcc caaggggata ctcgacaagg agatcgccca
      541 tttcgccaag atgggcggca ttgccatcca ctgcaacaag gaactcggca aggatctcgc
      601 catcgaggac ctgcagaaga agtacgacgc cgtcgcgata acggtcggct cgtggaagtc
      661 gagcgcggcg ggtgtcgagg gggaggaact cgccgtcggc ggcatccagt acctcgaaaa
      721 gatcgcgctc gccggctgga aaggcgacaa tcccggcaag accatcgtca tcggcggcgg
      781 caacaccgcg atggactgcc tgcgcacctc ggtgcggctc ggctccaaag aggtcacctg
      841 tttctaccgc cggaccgagg ccgagatgcc ggccgaaaag atagagatcc atgaggcgaa
      901 ggaagagggg gtcaagttcg aattcctcgt cgcgccggtg aaactgcgca aggagaacgg
      961 gcggctcgtc ctcaccagcc agcggatgac gctcggcgag cccgacgcct cgggccgtcg
     1021 caagccggtc ccggtcccca acagcgattt tgacaccgtc gccgacaccg tcatcgccgc
     1081 catcggccag aagacgatcg cgccgaaagg agtgaaggcc aacaaatggg gcgatgtcga
     1141 ggtgagcaag gagaactgcc gcatggaagg cagtatctac gcggcaggcg actgcgtcag
     1201 cggtcccgcc accgtcgtcg aggcggtcgc cggggcgcgc cggacggcgc tggccatact
     1261 tgcgcagata gagagcaaag aattcaagga accctatctg ctgaacgttt cgcgcggcaa
     1321 gtggcagagc ctcaagacag acgatctcgt gttcctcaag gacccgatcg aacgcaaacg
     1381 gacgccgcag cggctcatcc cggaacacga ccgcgtgacc accttcaagg aggtatcgct
     1441 gacctacacg caggccgaga taaccaagga gggcgaacgg tgcatcgaat gttcctgcac
     1501 cgcgaagggc gactgttcgc tcaagaaaca cagcgagacc tataccgcca agcccgacgc
     1561 gatggtcggc gaaaagccga acttcggcta cgatgtgcgg caccccgaga tcatcctcga
     1621 tcgcggcaag tgcatcaagt gcggcgtctg tgtgaaggtc tgtggcgagg tggtcaacca
     1681 gaacctcctc ggcttcaagt tccgcggctt cggcacccat gtcgacacgg cattctccca
     1741 gccgctcccg tcgcaactct gccgcgactg cggcaagtgc atcggcgaat gtcccgtcgg
     1801 cgccctcgac tggaaattga agaagtaacc gattattttt aaccgcaaaa ctcgacaact
     1861 tctcccgatg tatttatagt tccttgttca ttgacggcaa gggaacgcgc catgacccgc
     1921 gagaacaaga ccgcgaagta tgaccgcatc gtcgcccaac tggaggaact ctacctcaag
     1981 acgcccgacc cgatcgcacg gatggcgacg gcttccgcac tactcaaagg gaagttcaaa
     2041 tatttcttct gggtcggatt ctaccggctc gttggcggtg aactgacggt cgggccgtat
     2101 cagggggcac tcgcctgtca ggtgcttaaa aaacacaccg gcgtctgctg ggccgcgata
     2161 gatcgcggcg agacggtgct cgtccccgat gtcgaggcgt tccccggtca catcgcctgc
     2221 gacagccgtt cgaggtccga ggtcgtcgtg ccgctacgtg accgcgacgg tcgcatctgg
     2281 gcagtgctcg atgtcgattc ggatgaactc gattcgttcg acgccgacga tgtcgccgga
     2341 ttaaagaaag tggcggcgct tatcgatccg taagattttt tctgttatat cccggggaaa
     2401 acatggtcag gaacagctac cccactggtc gggatccgaa caactgtcaa agctgaagcc
     2461 gtcacaggtg ttgttgcacg gaacggtgcc gcatttgttt attgcgtcgc agttcatggt
     2521 ctgtcctccc gcttcgcatt gttcggtcgt agcttcatgg aatcggatat cgtccacata
     2581 gaaggcgtcc cagccgacat agttcgacgt gtccttatag tactcgaact ccagggtctg
     2641 gaagccggcg gtcgtgggga ggcattgttc gctccatgtc cgtttgtccc ccgaagtttc
     2701 gaagagcttg gagccgttga tgtacactct gaatttatcg aacgattcag attgtcccgc
     2761 gtaccagaaa catatctggc catcggtata acgaagcatt gataccgaag atgttccacc
     2821 atcgtccggt acggaacccg atttgagcga gtaggacccg gtatgtttct gggtgctcga
     2881 agtatcccag tcaccgttcg aatagttcgg caggccccct tcgaaggtct cggtgaatgc
     2941 gtcgataagg ccgccgggca cgccgtcgcc gcagacattt gccgcgcagg tatgggtcac
     3001 gatgctgcag acctcgcctg cgccggtgtt gcaatcatta ttgtcgcgac actgaacgca
     3061 ggtgttgttc gaggtgaggc agtgcggcgt gccgtcgccg cagtggccct cctcgaggca
     3121 ctgaacgcag gcactggtcc cgttcccgta gtctcgacag tagggggcgc ccgatccgca
     3181 ggcggcgcag gccgccccgc actgtcccgc ggtattgcag aaggtacagt cgtagccacc
     3241 tcccgtgtag tagtcattac aaatgcaggt gaatccgggt gtcgcgtcgg cgcaatgggc
     3301 gttcgaggga caatcgtggc ttacttcggt acattcatcg atgttgcggc aggatccggt
     3361 caggaactcg aacccccacg aacaggagca ttcatagccg cccagcgtgt cgttgcaggt
     3421 cgccgcagcg tctccgttgt cgtcgcaagg tcctttgccg tcgggggcgc attcgttgat
     3481 ctcctcgacg gccgtcgtga aggagaaaac atgatcgacc gtcaagccgt tgcccgcctc
     3541 gtcctgtgct ccggtcgtca ccgtgacggt gtaggtggta tcgtaggcga gcacggatgc
     3601 cggcgtgaag gtggcggtca tgctgccgga atcgtacgag accgcaccgt tgacgggggt
     3661 cgcgtcttgt ttgaccgtca tcgtggtggg ggtcagggtg agggcattca tgggttccga
     3721 gaaggtcgcc gccaccgtgg tcgcgatgcc gacgccggtc tcgttcgccg cgggggaggt
     3781 cagggtgacc gtcggcgggg tcgtgtcgct gttctgatct atgtcgggca gagggccgtc
     3841 atcgaaagga agagtgtcat cgagagggag ggcgtcatcc ttgatcgtca cgtcgtcttc
     3901 ggtgcctgct tcgatgtcct cgtcgcccaa cggtcccgtg ccgtcgatgt catcgttgtc
     3961 ggacgctagg gtgtcttgcg gtatctcatc ctcgtccggg tccgacgagg tgtcgttgaa
     4021 ttggtcatcg tcgatcggtg cgtcctgttc gatcgcttcg tcactcacgt ccgcttccgg
     4081 catcgcttcg gcctcctcgt cggtggcctt atcggtgacg atcgtttcgg tgtccgttcc
     4141 gccgctctgg tcgggagact cgacacagaa gccgtcctcg cagacaagac cgtcaccaca
     4201 atcgaacgcc gtgacgcatt ctactccttc cggttcatcg tatgagcagg ccgacaacca
     4261 caataagggg aggataaaaa aggccatctt gaacgacgaa tgatgtggtg atatcatggg
     4321 gtcctccctg aacgaaacct ctgttcctat catatgccgt ccatctcgaa gttgtccacc
     4381 cacgcggtgt agtgtgtcgt caccttcggc gaatagtggg cccaacggac cttgaaggcc
     4441 gagccggggg tgacatgcgc cgaaaggtcg gtcgattcac cgtagatgga ggatgtgaag
     4501 gaggtccagg tcttcacggt cgtccatgag gaacaggatc cctgacagac ctccagtttc
     4561 agtgaatcgg cggtggtcgc gttctcttgg aaccggtagt cgtaggtgat cgtgagggag
     4621 cagccgcttt tccggtgata ttccgggctg accagatagg cggtgctcgt ttcagcgttg
     4681 gtcggtggct cgtgcgcggc gcgattgtcg taaatagcgt gagagtatat ccagttgatg
     4741 cacccagtgc ctgtgcaact cagcgtccaa ttgttgcccc gcgggtcggt ccatgtgccg
     4801 tcgaagccca tcgtcacact ctcgcatgtt accgcggtgt cctcgtcggg ctgttcgtca
     4861 acggcttcga tgtcgaccgc gtcgtcggtc gcggtgtcgt cggtcacggt gtcgtcggtc
     4921 atagtgtcgt cggtcacagt gtcgtcggtc acagtgtcgt cggtcacagt gtcgtcg
//
LOCUS       DAOQUE010000181         4971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18753_length_4971_cov_3.222945, whole genome shotgun sequence.
ACCESSION   DAOQUE010000181 DAOQUE010000000
VERSION     DAOQUE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18753_length_4971_cov_3.222945"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKH10_06685"
     CDS             <1..464
                     /locus_tag="PKH10_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNT27845.1"
                     /translation="SVSGSAVTSPTGDFIVWKKDGRPSYQLASVVDDEHFAVTHIVRG
                     ADLRDPTAMQMILAGALGYTYFPRVRFLHHPLLVDRDGRKFSKSDGSLSLRALREGGV
                     TAAALYRWVAGTLGVTAKVAQAQELLDACDENRLMALDISVRGEAPIPVAL"
     gene            complement(461..1054)
                     /locus_tag="PKH10_06690"
     CDS             complement(461..1054)
                     /locus_tag="PKH10_06690"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNT27846.1"
                     /translation="MIEIPRRGSPVAHPIATVNWPDFPACCSASFRISWDGERIVLLF
                     EVRDLAVRAVETADNGPVWHDRCVEWFLSPCGESYYNMEWNAAGTMLCQHGPSRRDRV
                     FLPPERLALVERRSSLGPGPIPLTTGPLDWSLEVKIPLSFFGDDARRISPGATVRGNF
                     YLCGDDLPDKQYLSCFPIRSERPDFHRPECFGPLRFL"
     gene            complement(1051..2088)
                     /locus_tag="PKH10_06695"
     CDS             complement(1051..2088)
                     /locus_tag="PKH10_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT27847.1"
                     /translation="MQRNEATDRIYQLEKELLDLRENAASFEDLIMSSPIGVYRTTPD
                     GRVLVANQALAELMGYASVKDLLQQNISEQGYAIDDYRSIFKETIERLGRVTGFESAW
                     RRRDGSLIYVREYARVVRDESGAVLYYEGTIENITEKTKTETLLKESLRQIRRTFQEL
                     VETLSEAMNIRDPYTADHEKRVAALSYAIAVELGHSDGFANILRTAALLHDIGKIYVP
                     AELLSRPGDLRKEEMDLIKVHAEAGWEILKNVSFDQPIAEMIRQHHEKLDGSGYPRGL
                     KGDEIMLEARILSVADIVEAFSSHRPYRPAFGVPQALDQIEHMAAEGKLDRKVVAACL
                     RLFREKGFVLP"
     gene            complement(2158..3114)
                     /locus_tag="PKH10_06700"
     CDS             complement(2158..3114)
                     /locus_tag="PKH10_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT27848.1"
                     /translation="MKRTNAHIYLFAVLSMCFWAMSFVWVKIVYKYYEPLTTITLRLL
                     IASLLLWTIAPLFRVPLAIRKGDGKWFLLLAFTEPFCYFLGEAFGMKYVSPAVGSIIV
                     STIPLFCLLPGIFLLKERLSITNIGGIIISFCGILMMIHVRGYGFGQSPDAPLHGVLL
                     MFFAVFSAVGYSMLIKRLADRYHAVMLVAWQSFLGAIYFLPFFYVFDHDKFRAVTPTG
                     ELIGALLTLSVFASVLAFLFFMTGMRLANTLALVIQRIGIAKANVFANLIPVMTAVFS
                     YFVLAEAFTAQKLGGMALVIGGLFLSGTNTGKRTDADGVKRP"
     gene            complement(3183..>4971)
                     /locus_tag="PKH10_06705"
     CDS             complement(3183..>4971)
                     /locus_tag="PKH10_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27849.1"
                     /translation="MTGGPFMFGITVNDGCQTVTGSFTIDQVALSNLAGEEYLYLLSD
                     TDMFFIIWPQENDGEVIGETNINFPIGLEMNPVDGKIYTFDADTGYIIELDPDNGPAP
                     VVEVQYGDEIYDITFGPDGTLYAVAYNGIITIDMTTGEIEIFCDDGYYEWAASDSITY
                     SNGYLYTKAYGEEIFRTNIVTGVVSHVSGLNFPDIGEYDWCYQEKGLDYNFKDGKFYS
                     TMYDDNSGDHFLTVIDPATWVATRVMYIPPDFTGLAVPALDRTAPAEVEDLAIDEDQS
                     GCHHAKLTWTDPDDADLDFVMVSWTPNGPAEPVVVQEGYGEAIIIDDIDPETEYTFTV
                     RTVDTEGNMSEGVTFTVTSPACPVIAAPTHPLYLLTGAGSDTKGLLYTLDPLTLETTF
                     IGDTGLEATAGLAINPVDGKMYVTSNENGDLYEIDPATAEATLIGAIGVGNIPDITFG
                     ADGTLYGWDRGSNDLITIDLTTGAATIVGDSGIGSWAVGLAFDGDTLMLKSYDDDSDE
                     NMYTVDTTTGAVTLVSAISGIIVFPDNFYWLGNVLAIDDNDYYYTVQRDDYPGGNSYL
                     YAIDPDDNKATFLGDLGIIGVSALEFDKH"
BASE COUNT          981 a         1418 c         1587 g          985 t
ORIGIN      
        1 tctccgtctc aggctccgcc gtgacaagcc cgacaggcga cttcatcgtc tggaaaaagg
       61 acggccgccc ctcgtaccaa ctcgcctcgg tggtcgacga tgagcatttc gccgtcaccc
      121 atatcgtccg tggcgccgac ctgcgcgacc cgacggcgat gcagatgatc ctcgccggtg
      181 cgctcgggta cacgtatttc ccgcgggtcc gcttcctgca tcacccgctg ctggtggacc
      241 gcgacgggcg caaattctcg aaatcggatg gctcgctctc gctccgggcg ctccgcgagg
      301 ggggcgtcac cgccgcggcg ctgtaccgct gggtcgccgg aacgctcggc gtgaccgcga
      361 aggtggcgca ggcgcaggaa ctgctcgacg cctgcgatga gaaccgtctc atggctctgg
      421 acatctccgt tcgcggggag gccccgatac cggtcgcgct ctagaggaac cgcagggggc
      481 cgaagcactc gggccggtgg aaatcgggcc gttcgctccg tatcgggaag caggaaagat
      541 attgtttgtc cggcagatcg tcgccgcata gataaaaatt accccggacg gtcgcgccgg
      601 gagatatccg gcgggcatcg tcgccgaaga aggagagcgg tatcttgacc tccagcgacc
      661 agtcgagcgg gcccgttgtc agcggtatcg gccccggacc gagcgacgag cggcgctcca
      721 cgagggcgag ccgttccggc gggaggaaca cccggtcgcg tcgcgacgga ccgtgctggc
      781 agagcatcgt ccctgccgcg ttccattcca tgttgtaata gctttctccg catggcgaca
      841 ggaaccattc gacacaccgg tcgtgccaca ccgggccgtt gtcggccgtt tcgaccgccc
      901 gcaccgccaa gtctctcacc tcgaacaaca gcactatccg ttcgccgtcc cacgatatgc
      961 ggaacgaggc ggaacagcag gccgggaagt cgggccagtt gaccgttgcg atggggtggg
     1021 cgaccgggga gccgcggcgg ggtatctcta tcacggaagg acgaatccct tttcgcggaa
     1081 cagacgcaga caggcggcga ccaccttccg gtcgagtttc ccttcggccg ccatgtgctc
     1141 tatctggtcg agcgcctgcg ggacaccgaa cgcggggcgg tagggacggt gcgacgagaa
     1201 ggcctcgact atgtcggcga ccgacaggat gcgcgcctcg agcatgatct cgtcgccttt
     1261 cagaccgcgc gggtagccgg agccgtcgag tttctcgtgg tgctggcgta tcatctcggc
     1321 tatcggctgg tcgaacgaca cattcttgag tatctcccac ccggcctcgg catggacctt
     1381 gatgagatcc atctcctcct tgcgcagatc gccggggcgc gaaagcaact cggccgggac
     1441 atagatcttg ccgatgtcat gcaggagcgc cgcggtccgc aggatgttcg cgaacccgtc
     1501 cgaatggccc agttcgacgg cgatggcgta cgaaagggcc gccacccgtt tctcgtggtc
     1561 ggcagtgtag gggtcgcgga tgttcatcgc ctcggagagc gtttcgacca gttcctgaaa
     1621 ggtgcgccgt atctggcgga gactctcctt gaggagcgtt tcggtcttgg tcttctcggt
     1681 gatgttctcg atggtccctt cgtagtagag taccgcgccg ctttcgtcgc ggaccacccg
     1741 tgcatattcg cggacataga tgagcgaccc gtcccgacgg cgccacgccg actcgaaacc
     1801 ggtcacccgg ccgagccgct cgatggtctc cttgaagatg gagcgatagt cgtcgatggc
     1861 gtagccctgc tcgctgatgt tctgctggag caggtcctta acgctcgcgt accccatgag
     1921 ttcggcgagc gcctgattgg cgacaagtac gcggccgtcg ggggtggtgc ggtacacgcc
     1981 gatgggggag gacatgatga ggtcttcgaa cgacgcggcg ttctcgcgca ggtccaataa
     2041 ctctttttcg agttggtata tccggtcggt cgcttcgttc ctttgcatcg cgtgaccttc
     2101 acaaattgtc gccagtataa cagaaaagag cgaccgcgca agataaattt cgaggcgtca
     2161 cggccgcttg accccgtcgg cgtccgttcg tttgcccgta ttcgtccccg aaaggaacag
     2221 cccgccgatg acgagcgcca tgccgccgag tttctgcgcc gtgaacgcct cggcaaggac
     2281 aaagtaggag aacacggcgg tcatgaccgg tatgaggttg gcgaacacat tggccttggc
     2341 gatgccgata cgctgtatca ccaaggccaa tgtgttcgcc aacctcatac cggtcatgaa
     2401 gaacaggaag gcgagcaccg aggcgaacac ggaaagcgtg agcagggcac cgatgagttc
     2461 gccggtcggg gtgacggcgc ggaacttgtc atggtcgaac acataaaaga acggcagaaa
     2521 gtagatcgca ccaagaaaac tctgccatgc gacgagcatc accgcatggt atcggtcggc
     2581 gagtcgcttg atgagcatac tgtagccgac cgccgagaat accgcgaaga acatgaggag
     2641 cacgccgtga agcggcgcgt cgggcgactg gccgaacccg tagccccgca catgtatcat
     2701 catgagtatg ccgcagaaag agatgataat gccgccgata ttggtgatcg agaggcgttc
     2761 cttgagaagg aatatccccg gcagcaggca gaagagaggt atggtagaga ctattatgga
     2821 gccgacggcc ggggaaacat atttcatccc gaaggcctcg ccgagaaagt agcagaacgg
     2881 ctcggtgaat gcaagcagga ggaaccattt cccgtcgccc ttccgtatcg cgagcggtac
     2941 ccggaagagc ggcgctatcg tccagagcag gagcgatgcg atgaggagtc gcagggttat
     3001 cgtggtcagc ggctcatagt acttgtagac tatctttacc cagacgaacg acatcgccca
     3061 gaagcacatc gacaggacgg cgaacagata gatgtgagcg ttcgttctct tcacgatact
     3121 cctcgttccg gcggcctatc atggccgccg gaacggggaa gtcaaattaa atcggggaag
     3181 gtctagtgct tgtcgaactc gagggccgag acgccgatga taccgaggtc gccgaggaag
     3241 gtcgccttgt tgtcatccgg atcgatggcg tagagataac tgttaccgcc ggggtagtcg
     3301 tcgcgctgta ccgtgtagta atagtcgttg tcgtcgatgg cgagcacatt gccgagccag
     3361 taaaagttat cgggaaaaac tattatgccg gagatcgctg agacgagagt gacggctccg
     3421 gtggtcgtgt ccaccgtgta catgttctcg tcggagtcgt cgtcgtagga cttcagcatg
     3481 agcgtgtcgc cgtcgaacgc aagcccgacc gcccaagagc cgatgccgga gtcgccgacg
     3541 atggtcgcgg cgccggtggt cagatcgatc gtgatgagat cgttactgcc tctgtcccag
     3601 ccatagagcg taccgtcggc cccgaaggtg atgtcgggga tgttgccgac gccgatcgcg
     3661 ccgatgagcg tcgcttcggc ggtcgcaggg tctatctcgt acaggtcgcc gttttcgttc
     3721 gaggtgacat acatcttccc gtcgaccgga ttgatggcca gaccggcggt cgcttcaaga
     3781 ccggtgtcgc cgatgaatgt cgtctcgagc gtcagaggat cgagcgtgta gagaagtccc
     3841 ttggtgtcac tgccggcgcc ggtgaggaga tagagcgggt gcgtgggggc ggcgatgacc
     3901 ggacatgcgg ggctggtcac ggtgaaggtg acgccttcag acatgttgcc ttcggtatcg
     3961 acggtgcgca cggtgaaggt gtattcggtc tcgggatcga tgtcgtcaat gatgatcgct
     4021 tccccgtacc cttcctgtac gacgacgggt tcagccgggc cgttcggcgt ccaactcacc
     4081 atgacgaagt cgaggtcggc atcgtccgga tcggtccagg tcagtttggc atgatggcaa
     4141 ccggattgat cttcgtcgat cgcgaggtcc tcgacctcgg ccggggcggt ccggtcgagc
     4201 gcggggacgg ccaggccggt gaagtctggc gggatgtaca tcacgcgggt cgccacccaa
     4261 gtcgccggat cgatcacggt gaggaaatgg tcgccagaat tgtcgtcata catggtggag
     4321 tagaatttgc cgtccttgaa gttatagtcg aggccttttt cctgatagca ccaatcgtac
     4381 tcgccgatat cggggaaatt gagtccgctt acatgggaaa cgacaccggt gacgatattc
     4441 gtcctgaata tctcttcccc gtaggccttg gtgtagagat agccgttgct gtaggtgatg
     4501 ctgtcggagg cggcccattc atagtagcca tcgtcgcaga atatctcgat ctcgccggtg
     4561 gtcatatcga tggtgatgat gccgttatag gcgacggcgt acagggtacc gtcggggccg
     4621 aaggtgatgt cgtatatctc gtcaccgtat tgcacctcga cgaccggcgc cgggccattg
     4681 tcgggatcta actcgataat ataaccggtg tcggcatcga aggtgtatat ctttccatca
     4741 accggattca tctcaagtcc tatcggaaag ttgatgttcg tttcgccgat gacctcaccg
     4801 tcattttcct gcggccagat gataaagaac atgtcggtat ccgaaagcag atacaggtac
     4861 tcttcgcccg ccaggttgct gagggccacc tgatcgatgg tgaaggatcc cgtgacggtc
     4921 tgacagccgt cattgaccgt aatgccgaac atgaaggggc cgccggtcat c
//
LOCUS       DAOQUE010000182         4953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18846_length_4953_cov_3.856064, whole genome shotgun sequence.
ACCESSION   DAOQUE010000182 DAOQUE010000000
VERSION     DAOQUE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18846_length_4953_cov_3.856064"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..2446
                     /locus_tag="PKH10_06710"
     CDS             83..2446
                     /locus_tag="PKH10_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27850.1"
                     /translation="MRRELTAFFIFSMAILAVELSFTKVFSVILWYHFGFLIISTAML
                     GFAVAGVYLAVRGERVRAVPASLMTLAGAATWCAYALMTRVAAVSEALFPSRMLSSVK
                     MVEMVVIVVLLFLPFFFLGLTVARVIAVRRRDAGLYYGANLVGSAAGALLFLFLFDHF
                     PGDIAVPLTVLLMFAAGLVIAERPRERAVAAVSAALTLPFLFIPGAFPLDPPRDKMLG
                     FVKDPVAAVSYTGWSSLSKVDFVRDPDEHIAPGCGLWGISERFMAEGRPWPARTGIVI
                     DSWAYTSMIHYPQDLSFYDYLPTTFAFALRDYDSSLHIGAGGGMDLLAARHYGVRSVQ
                     GVEINPVIVRTIAEKFGDQAGGIYNGGMPGVEIFVDEGRHFVERADRSWDLIQLSGVD
                     TYSSTQAGAFALSENNLYTVEAFLSYYRKLNEGGMLTMTRWFAPDEELNLRYELRLLN
                     IAREALLRLGAGVNGNIFYFVSQNYTLLTIKKGGFSEEEIERGAAFIEKNRFIPIIVP
                     GRSFAWSKRFEDFLYAAPRDFDHMVADHPYNVEPPTDDKPFYFEIRRFSTLLSGMLNP
                     VAPFNELSGQTILLAVLTELAVLGILLILLPLRSLARRGAVRPAPSDIAYFSLIGLAF
                     MLIEIALSQKLVLYLGHPVYALSVVVFSMLLFSGIGSVACQRLSATRRRSALLFLPVV
                     IAVVLFLLPFITEATLHSGFPVRFLIALLLGGLPAFFMGFPFPAGILRSGEREVPVFW
                     GVNGFFSVVASVVAVQISINLGFTAVFVLALGSYVAASALYLRRREG"
     gene            complement(2450..>4953)
                     /locus_tag="PKH10_06715"
     CDS             complement(2450..>4953)
                     /locus_tag="PKH10_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002963659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT27851.1"
                     /translation="GKGANLAEMSRIGLPVPPGFTITTEVCTYYYANRRTYPKVLDAQ
                     VKANLLFSYDKRTEPFESVAERIEALRRQHPQDPDLAKALAFVLTAQQDHARAAELIE
                     SIPDKNDPELFFLLGLIAKARKDLQNAESLFSSGYIASQTKSNKLAFAIAELKMMQGD
                     VDGAVAFLNRIISASPYYIKAYLKKAEALAYEKKNIGDAITFLASIDANVLSQADETE
                     KAVYYALLGDMHYRNGATAEAIESYLRVVALDKENIRYLADLAKIYQETKQSSAALDY
                     YEKALAVDNRYIPAIIGKAEVFIRLKRYNDAFLEIAKIDITNLNDANQLLRLANLFHQ
                     QGEREKAMQLYERAITADPSLIDAYLGRVFLYLEMNMYDEIRKLLASIGELNRESHSY
                     YLIQGILNHRDGNYKKAEESFRKSLELNRSEDPRVFLFYGRFLYDRQNFPQASEYFAK
                     AVKISPDDPEYRVALARSLEKEKKYQDMLPLLDPVEGNQKVHAQAFQLRAEALMQTGD
                     LDNALKLIDRAIAIDAKNAYFFYKKADILFHREEMNPAISVIETALLLDINSFESYVL
                     FSKILLKKGDFKTAVEKLEEAARIDATSQQVYLLKGILLKNLDNYPEALRNFRKIRNN
                     PELEKEALLEIAECYLNLEKTDEALKYFSKALKAGNKTALRFLGRIYYDKGKLDKAAE
                     YYRQSLKVFPDDHEPLKQLGYIFKERGRHTTARSYFKRYIKFLRAGDPERAMIEDEIY
                     YSEKNIGTKKLSKIKKEEAEEESVGDDEFGEDAEGKTEEAKKLYMEAVSLAEADKKEA
                     KRILRKVMKTVPANDKYHIKARKLLKKIQEDEEEY"
BASE COUNT          791 a         1393 c         1465 g         1304 t
ORIGIN      
        1 atgagcgacg cggacttcgc ctttaccgga tagggtcgcg tgcctacgag ttccgttaac
       61 caccgccgga ggtgataccg atatgcgccg tgaactgaca gcctttttta tcttttcgat
      121 ggcgatactc gcagtggagc tttccttcac caaggtgttc tcggtcatcc tctggtatca
      181 tttcggtttc ctcatcatca gcaccgcgat gctcggcttc gcggtcgcgg gcgtttatct
      241 tgccgttcgc ggcgagagag tccgtgccgt cccggcatcg ctcatgaccc tcgcgggggc
      301 tgcgacctgg tgcgcctatg cgctcatgac ccgcgttgcg gcggtgagcg aggcgttatt
      361 cccgtcacgc atgctgagca gcgtcaagat ggtggaaatg gtcgttatcg tcgtacttct
      421 gttcctgccc tttttcttcc tcgggcttac ggtcgcgcgc gttatcgcgg tccggcggcg
      481 cgacgccggt ctttattatg gcgcgaatct ggtcggttcg gccgccggtg cgctactctt
      541 cctgttcctt ttcgaccact ttccaggcga tatcgcggtg ccgctgaccg tgctacttat
      601 gttcgctgcc ggacttgtta tcgccgaacg tccgagagaa cgcgccgtgg ccgccgtgag
      661 tgccgcgctg acgcttccgt tccttttcat cccgggagca ttcccgctcg atccgccacg
      721 agacaagatg ctcgggttcg tgaaggatcc ggtcgccgcc gtttcttata ccgggtggag
      781 ttctctttcc aaggtcgatt tcgtgcggga tcccgatgag catattgccc ccggctgtgg
      841 tctgtggggt atatctgaac ggttcatggc cgaaggtcgt ccctggcccg cgcggaccgg
      901 catcgtcatc gattcgtggg cctacacctc catgatacat tatccgcagg acctctcctt
      961 ctatgactac ctgccgacca ccttcgcctt tgcgcttcgc gactacgatt cttctctgca
     1021 tatcggcgcg ggcggtggca tggacctgct cgcggctcgt cactacggag tgcggtcggt
     1081 gcagggggtc gagataaacc cggtcatcgt gcgtacgatc gccgaaaagt tcggcgatca
     1141 ggcgggcggc atctacaacg gtggtatgcc cggggttgag atcttcgtcg acgaaggccg
     1201 acatttcgtc gaacgggccg accgctcgtg ggatctcatc caactgtcgg gggtcgacac
     1261 ctattcctcg acgcaggcgg gggcctttgc cctgtcggag aacaacctct ataccgtcga
     1321 ggcattcctt tcctactacc ggaaactgaa cgaggggggg atgctcacga tgacccgctg
     1381 gttcgcgccg gacgaggaac tgaaccttcg ctacgaactg cgcctgctca atatcgcgcg
     1441 agaggcgttg ctccgactcg gcgccggcgt gaacgggaac atcttttact tcgtatcgca
     1501 gaactacacg ttgcttacca tcaagaaagg tggtttctcg gaagaggaga tcgagcgtgg
     1561 tgcggccttc atagaaaaga accgttttat tccgatcatc gtgccgggtc gctcgttcgc
     1621 gtggagcaag agatttgagg atttcctgta tgccgcgccg cgcgatttcg atcatatggt
     1681 ggctgaccac ccgtacaatg tcgagccgcc gaccgacgat aagccgttct atttcgagat
     1741 acgccgtttc tcgacgcttc tttcggggat gctcaatccg gtggcacctt tcaacgaact
     1801 ctcggggcag accatcctgc tcgcggtgct taccgaactt gcggtgctcg gcatcctgct
     1861 catactcctg ccgctcaggt ccctcgcccg acgcggcgcg gtacgcccgg caccttccga
     1921 tatcgcctat ttctcgctca tcggccttgc cttcatgctg atagagatcg cgctgtcgca
     1981 gaaacttgtg ctctatctgg ggcatccggt ctatgcgctc tcggtggtcg tcttctcgat
     2041 gctccttttt tccggtatcg gtagcgttgc ctgtcagcgt ctttcggcga cgcgacgacg
     2101 gtcggcgctg ctgttccttc cggtggtgat agcggtcgtg cttttcctgt tgcccttcat
     2161 caccgaggcg accctgcaca gtggcttccc ggtacgcttt ttaatagcgt tgctgctagg
     2221 cggcctgccc gcctttttca tgggcttccc gttccccgcc gggatattgc ggtccgggga
     2281 gcgcgaagtg ccggtgttct ggggcgtgaa cggattcttc tcggtcgtcg cctcggtggt
     2341 cgcggtacag ataagcatca atctcggatt cacggcggtg ttcgtgctgg cgttaggttc
     2401 gtacgtggcg gcatcggcgc tctatctgcg ccgacgcgag gggtgaccgc tagtattcct
     2461 cttcgtcttc ctgtatcttt tttagcagct ttcgcgcctt gatatgatat ttgtcgttgg
     2521 cgggcaccgt cttcatgacc ttgcggagta tccgcttcgc ctctttttta tcggcttcgg
     2581 cgagcgatac cgcttccata taaagtttct tcgcctcttc ggtctttccc tccgcgtcct
     2641 cgccgaactc gtcgtcgccg acgctttcct cttcggcctc ttccttcttt atcttggaga
     2701 gttttttcgt gccgatgttc ttctcgctat agtatatctc atcctctatc atcgctcgtt
     2761 cgggatcccc ggctctcaag aattttatat agcgtttgaa atatgagcgg gccgtggtgt
     2821 ggcgcccccg ttccttgaag atgtatccca gttgtttcag gggttcgtgg tcgtcgggga
     2881 acaccttgag cgattggcgg taatactcgg ccgccttatc gagtttgcct ttgtcgtagt
     2941 agatgcgacc gaggaaacgg agcgccgtct tgttgcccgc cttgagcgct ttgctgaaat
     3001 atttcagcgc ctcgtcggtc ttctcgagat tgagatagca ttcggcgatc tcgaggagcg
     3061 cctccttttc gagttcgggg ttgttccgta tcttccgaaa gttccggagc gcctcggggt
     3121 agttgtcgag attcttgagg agtatcccct tgagaaggta gacctgttgg ctcgtcgcgt
     3181 ctatgcgtgc cgcctcttcc agtttctcga ccgccgtctt gaagtctcct ttcttgagga
     3241 gtatcttgga gaacagaacg taactctcga aactgtttat gtcgagcaga agtgccgtct
     3301 cgatgaccga tatcgccggg ttcatctctt cccggtggaa gaggatgtcg gcctttttat
     3361 agaagaagta ggcgttcttc gcgtctatcg cgatggcccg gtcgatgagt ttgagcgcat
     3421 tgtcgaggtc gccggtctgc ataagcgctt cggcgcgcag ttggaacgcc tgtgcgtgaa
     3481 ccttctggtt gccctcgacc ggatcgagaa gcgggagcat gtcctggtat ttcttctcct
     3541 tttcaaggga cctggcgagc gccacgcgat attcgggatc gtcgggcgat atcttgaccg
     3601 ccttcgcgaa atactcggac gcctggggaa aattctggcg atcgtacagg aaccgcccgt
     3661 agaataggaa cacgcgggga tcctcggaac gattgagttc caatgacttt cggaacgatt
     3721 cctcggcctt tttgtagttg ccgtcgcggt ggttcaatat gccttgtatg aggtaataac
     3781 tgtgcgattc gcggttgagt tccccgatgc tcgcaagtag tttgcgtatc tcgtcataca
     3841 tgttcatctc gaggtaaagg aatacgcggc cgaggtaggc gtcgatgaga gaaggatcgg
     3901 cggtgatggc ccgctcgtag agttgcatgg ccttttcccg ttccccctgc tggtggaaca
     3961 ggttggcgag tcgtagcagt tggttcgcgt cgttgaggtt cgtgatgtct atcttcgcga
     4021 tctcgaggaa tgcgtcgttg tatctcttca gacggatgaa gacctctgcc ttgccgatga
     4081 tggctgggat gtatcggttg tccacggcga gtgctttctc gtaataatca agcgcggcgg
     4141 acgactgttt ggtctcctga tagatcttgg cgagatcggc gaggtatctg atgttctcct
     4201 tgtcgagcgc gacgacccgc aggtaactct ctatcgcctc ggcggtcgcg ccgttccggt
     4261 aatgcatatc gccgagcagg gcgtaataga ccgctttttc ggtctcgtcg gcctgtgaga
     4321 ggacattggc gtcgatggat gcgaggaacg ttatcgcatc gccgatgttc tttttctcgt
     4381 aggcgagcgc ctccgccttc ttcaggtaag ccttgatgta gtagggactc gccgagatga
     4441 tgcggttgag gaacgcgacg gcgccatcga catcgccctg catcatcttg agttccgcta
     4501 tggcgaaggc cagtttgttg ctttttgtct gcgaggcgat gtagccggaa ctgaagagtg
     4561 actcggcgtt ctggaggtcc ttgcgcgcct ttgcgatgag tccgagcagg aagaaaagtt
     4621 ccgggtcgtt cttgtcgggg atcgattcta tcagttcggc cgcccgtgcg tgatcctgct
     4681 gtgccgtgag aacgaaggcg agtgccttgg cgaggtcggg atcctgagga tgctggcggc
     4741 gcagcgcctc gatgcgctcg gcgaccgact cgaacggttc ggtcctcttg tcgtatgaga
     4801 acaggagatt cgccttcacc tgcgcgtcca gcactttcgg gtaagtgcgc cggttcgcgt
     4861 agtaatacgt gcagacctca gttgtgatgg tgaaccccgg aggcaccggc agcccgatgc
     4921 ggctcatttc cgccaggttg gcacccttgc cgc
//
LOCUS       DAOQUE010000183         4936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18934_length_4936_cov_4.994059, whole genome shotgun sequence.
ACCESSION   DAOQUE010000183 DAOQUE010000000
VERSION     DAOQUE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18934_length_4936_cov_4.994059"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..838)
                     /locus_tag="PKH10_06720"
     CDS             complement(107..838)
                     /locus_tag="PKH10_06720"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT27852.1"
                     /translation="MMQKQGKREITTAYTSQQANIYDQSRFTKPQGVCVHQREWTTLR
                     TTLHLVKKEGRVLEVGCGTGRLLVEVQRLGYHVDGADASPDMLDKLKEKLGPDGSDTS
                     FWIAPADKLPCSNASYDLTYAIRLLNQTESPEYALGVVGEMIRVTKPSGYVLVEFVNE
                     WRPRWGMGKRKTTRLKPSEVAKAGIASGASPMAYYGNFFLSMQAFHYSPAFLLPLVNG
                     LDKLFSVLFPRFCSRCYVLFKKDAT"
     gene            complement(835..2070)
                     /locus_tag="PKH10_06725"
     CDS             complement(835..2070)
                     /locus_tag="PKH10_06725"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT27853.1"
                     /translation="MAVMFIMMVKYTHEPLDERVMKYDLVTWILFACYFFLSIKSIVQ
                     YFSKKDSILLSLSLFLFVVVFLSIFSQIPQMSLLKIVGFVWGIFTCYVLVRESEHPLY
                     EFLFGVLAFYVVAGTISFSYPEISVKEWTTLHQGITRHPQYYATLLSPLIALSVIRYL
                     KGFREKKQTAMSLFLLFFGMVQLFYTYSRTGMFSSFLAVAVTIVIMMYRERQRSIFLK
                     VLLILTLLLIPVLMKYDELTQAVSLIMTKEIKHEDAFDYSPEQFGIEDVVETRQGLID
                     FSYENFQEHPIVGIGFGVPSEIAYLKIASDDLFNIPLSAPIEKGNIFSTVLEENGLIG
                     FFFFLIFLFTLLRRIFSADYPDIALMVSAFLVNMGEMAFFSTNGPGPYIWLMMFSGLR
                     VPKMKTENIDNDHLIRRGE"
     gene            complement(2217..3548)
                     /locus_tag="PKH10_06730"
     CDS             complement(2217..3548)
                     /locus_tag="PKH10_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNT27854.1"
                     /translation="MPHAFLTNIKERFNALLSSPTRRRLASGAFWGGVAAAGARFVTV
                     ITSFFLARILGQIGFGEYGMITSTAIMVGSFAGMGIGLTVTKHVAEFKSKDPDRVGRL
                     LALSSIVTMASALIYGVAFVALAPWLAERTLAAPHLAPMLRISAISVAFSVVNGVQVS
                     SLAGVEAFRAKSHIMVGTGLLQTVLVVIGAWLWGLEGAVWAMAVGMLLTVIVTRWVVS
                     REWHRRNIRLLWREARREWRILLDFSLPAFLNSLLIGPVYWACNAFLANEPNGYAELG
                     IFNAAAQWQGAILFLPWLFITAMIPVISEKYGDGDVTGSLKVMADMMKLIVWAVVPIA
                     AVLSLSSPWVMSGYGDTFTAGYWTMVLLVATGVFNGIEAPIQHFAVASGQVWARLALY
                     GLWAAVLLAASWFMVKWGAEGLAGARLFSAVFHFVLFYLFIVTHQKKVTSC"
     gene            3865..>4936
                     /locus_tag="PKH10_06735"
     CDS             3865..>4936
                     /locus_tag="PKH10_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27855.1"
                     /translation="MKKLLVMLSVLIATAALYALNVWPQRNFDSARTGRTPGVGDIVT
                     PELKWTYFTGGSSSDATLRFDENSAQPFVFTMGGHVVRKGVNDTAVWDTGAVGLQNVF
                     GLFDVNGDGAREVFTYGTDTLRILDGATGNELFSHYLQNPAVWALADVDNDGDLELLL
                     RPRRAVGGLKVSEIADDLTNPTVKWEVTSGLPAWATRPVFGDLDGNPATKEMIFDSST
                     NNGRLWILNAATGQLLRYRSAKMTVGGFSGGLRLIGDFDTDPQQEYLFSGGTALYTDN
                     GSIQISVYEHLTDTVQWHYEYGAGTSDVKLDIVPESVADLDGDGNKEVVLSVYNDTLE
                     LVLVNGVRVDRDEDGINLPNRWV"
BASE COUNT         1329 a         1245 c         1273 g         1089 t
ORIGIN      
        1 gcgtcaagaa gcaattgcat gaccttgagt ggtatctgga tatgccccga aacattgttc
       61 tcgaacccct tctggcccga taccaccgag acatagtgcc cgatcttcat gtcgcatcct
      121 ttttgaatag gacataacaa cgggagcaga agcggggaaa caacacggaa aacaatttat
      181 caaggccatt gaccagaggc aataaaaacg ccggggaata gtgaaacgcc tgcatactca
      241 aaaagaaatt gccgtagtag gccatcggtg aggctccgct cgctattcct gccttcgcca
      301 cttctgatgg ttttaaccgg gtggtctttc gcttgcccat gccccaacga ggacgccatt
      361 catttacgaa ttcgaccaac acatagccac tcggcttggt cactcggatc atttcgccca
      421 ccacgccaag ggcgtactcc ggcgattcgg tttgattcaa caatcgtatg gcgtaggtaa
      481 gatcataaga agcattgcta cagggcaatt tgtcggccgg tgcgatccaa aaagaggtgt
      541 cgctaccgtc cgggccgagt ttctccttca gtttatcgag catgtcgggc gaggcgtcgg
      601 caccgtcaac atggtaacct aacctttgca cttctacaag caagcgtcct gttccacagc
      661 caacctctaa aacacgcccc tcttttttca caagatgtaa tgttgttcgt aaagtggtcc
      721 attctctttg atgaacacag actccttgtg gtttagtaaa tcgactctgg tcataaatat
      781 tcgcttgttg ggaagtgtat gccgttgtga tctccctttt cccttgcttc tgcatcattc
      841 acctcgtctt attaggtggt cattgtcgat gttttctgtt ttcatctttg gaacacgaag
      901 gccgctgaac atcataagcc agatataggg ccctggtccg ttcgtcgaaa aaaaggccat
      961 ctcgcccatg ttcacaagaa aggcactcac catgagtgca atgtcgggat agtcggctga
     1021 aaaaatccgg cgcaacaatg tgaaaaggaa gatcaaaaag aagaagaaac caatcaatcc
     1081 attttcttca agtaccgtcg aaaagatgtt ccctttttca ataggagcgc tgagagggat
     1141 gttgaataaa tcgtcacttg ctatcttcaa ataagcgatc tccgagggaa cgccgaaacc
     1201 gatgccgacg atgggatgtt cctgaaagtt ttcgtaggag aaatcgatta gcccttgtcg
     1261 agtttcgaca acatcctcta ttccaaattg ctcggggctg taatcaaaag catcctcatg
     1321 ttttatttcc ttggtcataa ttagggatac tgcctgagtt aattcatcat atttcatcag
     1381 gaccggtata agcaagagag tgagaatcaa caataccttt agaaagatag atcgttgccg
     1441 ttcacgatac atcatgatga caatcgtcac cgcgacagcg aggaaagagg aaaacattcc
     1501 cgtacgcgaa taggtgtaaa acagttggac catgccgaaa aaaagaagaa aaagagacat
     1561 tgccgtctgt tttttttccc gaaatccctt taaataccta ataacagaaa gtgcgataag
     1621 aggggaaaga agggtggcgt aatattgagg atgtcgggtt atcccttgat gaagagtggt
     1681 ccattccttg acacttattt ctgggtagga aaaggagatg gtgcccgcaa cgacataaaa
     1741 agcaaggacc ccaaaaagaa attcatacaa tggatgttcc gattcccgca caagaacata
     1801 acaggtgaaa atgccccaaa cgaaccccac tatcttaagc agagacattt gcggtatttg
     1861 actgaaaatt gaaagaaaaa ccacgacaaa taaaaacaag gaaagagaaa gcaagatgct
     1921 atcttttttt gagaaatatt gaacgatact tttaatcgac agaaaaaaat agcatgcaaa
     1981 aaggatccaa gtcactaggt catatttcat cacccgttca tcaagcggtt cgtgggtgta
     2041 tttcaccatc atgatgaaca tcactgccat taattgccag tacttcgagc ggagcgatat
     2101 cagaaaaaga atcccgaaaa tgagatagtt caccggttga taggtgaact ggaaaagcgt
     2161 caatatcgaa aggatcacta tcggggtaaa aaaacgaacg accgacgact cctgtttcag
     2221 cacgaggtga cctttttctg gtgggtgact atgaaaagat agaaaagaac aaaatggaat
     2281 actgccgaga aaaggcgcgc gccggccaat ccctcggcgc cccacttcac catgaaccag
     2341 ctcgccgcta acaacaccgc cgcccagagg ccatagagcg caagacgcgc ccagacttga
     2401 ccgctcgcaa cggcaaaatg ttgtatcggt gcctcgatgc cgttgaatac tccggtggcg
     2461 acgagcagga ccatcgtcca ataacctgcg gtgaaggtgt cgccgtaccc gctcatgacc
     2521 catgggctgg aaagagaaag aacggcagcg attgggacca ctgcccacac gatgagtttc
     2581 atcatgtcgg ccatcacctt caggctgccc gtcacatcgc cgtcaccgta cttctccgat
     2641 atcacaggga tcatcgccgt aatgaaaagc cacggaagga acagtatcgc cccctgccac
     2701 tgagccgcgg cattgaagat gcccagttcg gcatatccat tcggctcatt cgcaaggaag
     2761 gcattgcagg cccagtagac ggggccgatg agcagggaat tgagaaaagc gggcaggctg
     2821 aaatcgagca atatccgcca ctccctcctc gcctcgcgcc agaggagacg aatgttgcgt
     2881 cggtgccatt cccgcgaaac cacccatcgc gttacgatga ctgtcagcaa cattcccact
     2941 gccatcgccc agaccgctcc ttcaagaccc caaagccaag caccgatcac gaccaatacg
     3001 gtctgcaaca accccgtgcc taccattata tgagactttg cgcggaaggc ctcgaccccg
     3061 gccagagaac tcacctgtac accattgacc acgctgaacg cgacggagat ggcgctgatc
     3121 cttaacatcg gggcaaggtg cggcgccgca agcgtccgtt cggcgagcca aggggcgagc
     3181 gccacaaagg cgacaccata gataagcgcg ctggccatcg ttactatcga tgaaagtgcg
     3241 agaagtcgtc cgaccctatc agggtctttg cttttgaatt ctgcgacatg tttggtgacg
     3301 gtcaggccga tacccatccc ggcgaagcta ccgaccatta tggctgtact tgttatcata
     3361 ccgtactcac cgaatccgat ctgtccgagg atgcgggcaa gaaagaagga ggtgatgacc
     3421 gtcacgaatc gcgcgccggc tgctgcgaca cctccccaaa aagcaccgct cgccagacgg
     3481 cgtcgcgtcg gggaggaaag aagcgcatta aatcgttctt ttatattggt aaggaaagcg
     3541 tgcggcatga aaccccgttc aataacttgc gagattataa ggccgagcca tttcaaagca
     3601 agaaaatggg ggaagtgaag ggtgaagagt gagcggtgag cagtgaacgg tgaatccgaa
     3661 ttgtaggggc gatcccatcg ggacgccctg ttgaaaaaca tacatcatgt cggtttgaca
     3721 atcgagcggt ttcaaaagtt gctttttacg gatataccgg accctaagtg tccaaatata
     3781 tttttgatat aataggttga ctgtatcctc aatgtgaata gaatgggggt atgttctttg
     3841 gaattttatg ggatggagga tacgatgaaa aagttacttg ttatgttgag cgtgctgatc
     3901 gcgacagcgg cgctctacgc gcttaatgtg tggccgcaga ggaacttcga ctctgcccgt
     3961 accggccgga cgccgggggt cggggacatc gtgacgccgg aactcaaatg gacttacttc
     4021 accggagggt catcgtccga tgcaacgctc aggttcgatg agaattcggc gcaaccgttc
     4081 gtcttcacga tgggcggtca tgtggtccgg aaaggggtca atgacacggc ggtgtgggac
     4141 accggcgcgg tgggcctgca aaatgtcttt ggcctgttcg atgtgaacgg tgacggggcg
     4201 cgcgaggtgt tcacctacgg caccgatacc ctgcggatac tcgacggcgc gaccggcaac
     4261 gaactctttt cgcactatct acaaaaccct gccgtctggg cgctcgccga tgtggacaac
     4321 gacggtgatc ttgaattgtt gcttcgccct aggcgggcgg tcggcggatt aaaggtctcg
     4381 gagatcgcgg atgacctgac caaccccacg gtaaaatggg aagtgacctc cggccttccg
     4441 gcatgggcga cccggccggt attcggcgac ctcgacggta atcccgcgac caaagaaatg
     4501 atcttcgaca gttcaaccaa caatggtcgg ctgtggatac ttaatgccgc tacggggcaa
     4561 ctgctccgct atcgtagcgc taaaatgacg gtcggtggat tcagcggcgg attacgactc
     4621 atcggagatt tcgatactga tcctcaacaa gaatatcttt tctccggcgg aacggcgctc
     4681 tataccgaca acggttcgat ccagatctcc gtgtacgaac atctgaccga tacggtgcag
     4741 tggcattacg agtacggtgc gggaacctct gatgtgaaac tggacatcgt tcccgaatcg
     4801 gtggccgatc tcgacggcga tggcaacaaa gaggtggtgc tgtcggtcta caacgacacg
     4861 cttgagttgg tgctggtgaa cggtgtccgc gtggaccgcg acgaggatgg catcaacctg
     4921 ccgaaccgtt gggtga
//
LOCUS       DAOQUE010000184         4936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18937_length_4936_cov_3.707232, whole genome shotgun sequence.
ACCESSION   DAOQUE010000184 DAOQUE010000000
VERSION     DAOQUE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18937_length_4936_cov_3.707232"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PKH10_06740"
     CDS             <1..763
                     /locus_tag="PKH10_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNT27856.1"
                     /translation="DHHRETAQTGRGGQQPLMSGGLLILRESDNELALFKPAGLSSEF
                     KGDTDGVSAKALVAKRHPGADPKLPHRLDRITRGILLVCLSQKAIAFQNEQIKAGRWE
                     KYYLARVAAPTGKSPLEFIGEHMAYLKEVKGRAKLVHAGGKPSRLEILGAAPAPEMPE
                     RWHLLVRLLTGRFHQIRVMCAGMGLPLAGDPLYDPERRDPAEFYLEHIILKYTPFDTK
                     QPTTLFLPKLAERGPIAPELSELLAMIVEREPLFI"
     gene            complement(756..2771)
                     /locus_tag="PKH10_06745"
     CDS             complement(756..2771)
                     /locus_tag="PKH10_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNT27857.1"
                     /translation="MNRPAYQTPRWLLGALFLFWGVNSDMPVYGLTAALLVEGSYLIK
                     RKWELTRDDFIRVSDVSSFIMLGSILLIYLTNEPWQIMRRFVIWLPIIHLPLLLSQLY
                     STSDRIVIGTRLGLRKGREPHAHRPFNLTWIYAALLVFSAAAANSRAVWFFPFLVLFA
                     AWALFNYRGRRYPAALWTGGMAIVAVVSLFTALSLNNAFLYSQDRLMEYYQLWFARFY
                     ADPFKSDTNLGSLSAFKASSRIVMRVRPGDGPGAPYYLTDGVFDTYAVTSWYNRDPRS
                     RPVPLDENARWVLREPTPPVEARIDISAWLPRNKGVLPLPQGAHLVSRLNAAGVKELS
                     LGPVLVEEGPELLDYTAHYLPGADHAAPPSDRDLMVPTDELPGLDAAIAEMTPATGDP
                     RAIIDAIDHFFLFRFTYSLDAVPYAKQRTAVSNFLMNQRRGHCEYFATATVLLLRRLG
                     IPARYKTGFLVDEWSPIEETYVVRERHGHAWATAWVNGTWLDLDTTPPGWIVTESLAA
                     GPLERVRDLLAYLGYRYEKFRQLKDERVNQALLAGIVILSVILLYRIYAQRRRKEAGA
                     GDLTDLPRYDTPGKDSPFNEVAELLATVGIERLDHETYGRWARRIDEVRRGRGDANAP
                     FVDEEFVALLALHERYRFDPRGLTAKEKERFIASAAAWLERHRPPSR"
     gene            complement(2768..4093)
                     /locus_tag="PKH10_06750"
     CDS             complement(2768..4093)
                     /locus_tag="PKH10_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNT27858.1"
                     /translation="MRRRLYIWYRRTERFSRYLRERLTPVGMLVWVLFFAALIFGLNT
                     QKSMVFQILAILFSLLTLSRLADRSFSPRLAVRRHLPKYGAVGTPLRYPVTVRNLGDR
                     AEKGLTLRETPEDPTPSFEEWRDTPEPGEEERNRFDRLMRYYRWEWLIGKRIGATFEP
                     TELPTLAPGETRDIELTLTPTRRGVVRLAGLRAERSDPFGIWKRGLVVPADGRLTVLP
                     RLYPVNAPDFCGHRRYHPGGVPSAHKTGDSEEFTQVREYRPGDPLRKIHWRSTARTGM
                     LTVKEFQNEYFARVGIILDTFLKRSFSAVLEEAVSVAASYVARGGSDDEMVDLMFVGA
                     DAYSFTGGRGVASTDRMLEILAGVEPCRDRPFDDLLRVVAERAALLCGAVVVLTGWDD
                     RRQRLLETLDAARVEHLALLVTDNAATVPGAPPARVLEVGRIAEGLRRL"
     gene            complement(4090..>4936)
                     /locus_tag="PKH10_06755"
     CDS             complement(4090..>4936)
                     /locus_tag="PKH10_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNT27859.1"
                     /translation="AAFFSGGHVLLEDYPGTGKTTLAKTLARSIDIPFRRIQFTPDLL
                     PSDILGVSVYNQHDGKFEFREGPVFTNILLTDEINRASPRTQSALLEAMAEGQVSTDG
                     VRRPLPEPFFVIATQNNIESRGTYPLPESQMDRFMMRLSMGYIARDEEVAVMSAQGIE
                     HPLKQVRPVSDAAEVTAVLRAVRSVAISEEMKRYIVDLTAATRAAEGIALGASPRASI
                     ALMRTAQALALFDGTGFVTPDYVQEMAIPVLAHRLFLDPQARFAGLSAEAVVTRVLQG
                     IPAPV"
BASE COUNT          886 a         1529 c         1724 g          797 t
ORIGIN      
        1 tgaccaccat cgagaaactg ctcaaacagg ccgaggcggc cagcaaccgc tgatgtccgg
       61 cggactgctt attctcaggg aaagcgacaa cgaactggcc ctcttcaaac cggcggggct
      121 gtcaagcgag ttcaagggcg acaccgacgg cgtatcggcc aaggcgctcg tcgccaaacg
      181 acaccccggc gccgacccga aactccccca ccggctcgac cgcatcaccc gcggcatact
      241 ccttgtctgt ctctctcaga aggcgatagc gttccagaat gaacagataa aggcgggccg
      301 ctgggagaaa tactatctcg cccgcgtcgc cgcgccgacg gggaaaagtc cgttggaatt
      361 cattggtgaa cacatggcat atctgaaaga ggtgaagggg cgcgcgaagt tggtccacgc
      421 cggcgggaaa ccgtcgcggt tagagatact cggcgcggcg ccggcgccgg agatgcccga
      481 aaggtggcac cttctcgtcc ggttgctcac cgggcggttc catcagatac gggtgatgtg
      541 cgccgggatg gggttaccgc tcgcgggcga tccgctctac gacccggagc ggcgcgaccc
      601 ggccgaattc tacctcgaac acatcatcct taaatacacg ccgttcgaca ccaaacagcc
      661 gacgaccctc ttcctcccca agctggcgga acggggaccg atcgcccccg aactatcgga
      721 acttcttgcg atgatagtgg aacgggaacc gctgttcatc tagatggcgg ccggtggcgt
      781 tcgagccatg ccgccgccga tgcgatgaag cgttccttct ctttggccgt caacccccgc
      841 ggatcgaagc ggtaccgttc atggagcgcg aggagcgcca cgaactcttc gtccacgaag
      901 ggggcgttcg cgtcgccgcg cccccgccgc acctcgtcga tgcgccgcgc ccagcggccg
      961 taggtctcat gatcgagccg ctcgatgccg accgtcgcga ggagttccgc cacctcgttg
     1021 aacggtgaat ccttgcccgg cgtgtcgtac cgcgggagat cggtcagatc cccggccccc
     1081 gcctcctttc gtcggcgttg cgcatagatg cggtacagga ggatgacgga aagtatcacg
     1141 atgccggcga gtagcgcctg attgacccgt tcgtccttga gttgccggaa cttctcgtag
     1201 cgatagccga gatacgccag caggtcgcgc acccgttcca acgggccggc ggcgagcgac
     1261 tcggtcacga tccagccggg tggtgtcgtg tcgagatcga gccatgtccc gttcacccag
     1321 gccgtcgccc aggcatgacc gtgccgttcc cgcaccacat aggtctcctc gatgggactc
     1381 cactcgtcca cgaggaaccc ggtcttgtag cgggccggta tgccgagccg ccgcaggaga
     1441 agcaccgtgg cggtcgcgaa gtattcgcag tggccccggc gctggttcat caggaaattc
     1501 gacacggcgg tccgctgttt ggcatacgga acggcgtcga gcgagtaggt gaaacggaag
     1561 aggaagaaat ggtcgatcgc gtcgatgatg gcgcgcgggt cgccggtcgc cggcgtcatc
     1621 tcggctatcg cggcgtcgag ccccggcagt tcgtcggtcg gcaccatgag gtcgcggtcg
     1681 gagggcggcg ccgcgtggtc ggcgccgggc agatagtgcg cggtgtaatc gagcagctcg
     1741 gggccctcct cgacaagcac cggtccaagt gaaagttcct tgactcccgc cgcgttgagt
     1801 cgcgacacga ggtgcgcccc ctgcgggagc gggagcacac ccttgttccg gggaagccac
     1861 gcgctgatgt cgatacgcgc ctcgaccggc ggcgtcggtt cgcggagcac ccagcgggcg
     1921 ttctcgtcga gcggtacggg gcgggaacgg ggatcgcggt tataccacga ggtgaccgcg
     1981 taggtgtcga agacgccgtc ggtgaggtag taaggggcgc cggggccgtc gccgggacgg
     2041 acccgcatca cgatgcgaga agaggctttg aaggccgaca gcgagccgag gttggtgtcg
     2101 ctcttgaacg ggtcggcgta gaaccgggcg aaccagagtt gataatactc catcagccgg
     2161 tcctgcgaat agaggaacgc attgttgagt gagagcgcgg tgaaaagaga tacgaccgcg
     2221 acgatcgcca tgcctccggt ccagagcgcc gctgggtagc ggcggccgcg gtagttgaag
     2281 agggcccacg ccgcgaaaag cacaaggaac gggaagaacc ataccgcgcg gctgttggcg
     2341 gcggcggccg aaaaaacgag gagcgcggcg tatatccagg tgaggttgaa cggccggtgg
     2401 gcgtgtggtt cgcgaccctt gcgcagaccg agccgcgtcc cgatgacgat gcgatcgctc
     2461 gtcgagtaaa gttgggagag caggagcggc aggtggatga tcgggagcca gatgacgaac
     2521 cgccgcatga tctgccacgg ctcgttggtg aggtagatga gcaggatgga tccgagcatg
     2581 atgaacgaac tgacatcgct cacccggatg aaatcgtcac gggtgagttc ccatttccgt
     2641 ttgatgaggt atgagccctc gacgagtagc gccgccgtga gaccgtagac cggcatgtcg
     2701 ctgttcacac cccagaagag gaacagtgcg ccgagcagcc agcgcggtgt ctggtaggcg
     2761 gggcggttca tagccgtctc aagccctccg ctatcctgcc gacctcgagc acccgcgccg
     2821 gcggcgctcc cggcaccgtc gcggcattat cggtgacaag aagcgcgagg tgttccaccc
     2881 gcgccgcatc gagcgtttca agcaggcgtt gccgccggtc gtcccacccg gtcagcacca
     2941 ccaccgcgcc gcacaggagc gcggcccgct cggcgacgac gcgcaacagg tcgtcgaacg
     3001 gccggtcgcg gcacggctcc accccggcga ggatctcgag catccggtcg gtgctcgcga
     3061 cgccgcgtcc gcctgtgaac gagtaggcat cggcgccgac gaacatgagg tccaccatct
     3121 cgtcatccga cccgccgcgg gcgacatacg aggcggcgac cgacaccgcc tcctcgagca
     3181 ccgcgctgaa cgaacgtttc aggaaggtgt cgagtatgat gccgacgcgc gcgaaatatt
     3241 cgttctggaa ctccttgacg gtgagcatcc cggtgcgcgc cgtcgaacgc cagtgtatct
     3301 tgcggagcgg atcgccgggg cggtactcgc ggacctgcgt gaactcctcg gaatcgccgg
     3361 tcttatgggc cgacggcacg ccgcccggat ggtagcgacg gtggccgcag aagtcggggg
     3421 cgttcaccgg gtagaggcgt ggcagaacgg tgagccgtcc atcggcgggc acgacgagcc
     3481 cccgcttcca gataccgaac ggatcgctcc gttcggcccg cagaccggcg agccggacga
     3541 cgccgcgccg cgtcggggtg agcgtcaact cgatgtcgcg cgtttcgccg ggagcgagtg
     3601 tcggcagttc ggtcggctcg aaggtcgcgc cgatccgttt gcctatgagc cactcccacc
     3661 ggtagtaacg catgagccgg tcgaaccggt tgcgttcttc ctcgcccggt tcgggggtgt
     3721 cgcgccattc ctcgaacgag ggggtcgggt cctccggtgt ctcgcggagc gtcagcccct
     3781 tctcggcgcg atcaccgagg ttccggacgg tgaccgggta gcggagcggc gtgccgacag
     3841 cgccgtattt ggggagatgg cggcgcacgg cgagtcgcgg ggagaaggaa cggtcggcga
     3901 gccgcgagag cgtaaggagg gaaaagagta tcgcgagtat ctggaacacc atgctcttct
     3961 gggtgttcag cccgaagatg agcgcggcga agaagagcac ccacacgagc atgccgacgg
     4021 gggtgagccg ttctcggaga tagcgcgaga accgctcggt gcggcggtac cagatgtaga
     4081 ggcggcgtct cataccggcg ccgggatccc ttgcaggacg cgggtgacga ccgcttcggc
     4141 cgacagcccg gcgaaccggg cctgcgggtc gaggaacagg cggtgagcga ggaccggtat
     4201 ggccatctcc tgcacatagt cgggggtcac aaagcccgtg ccgtcgaaaa gggccagcgc
     4261 ctgcgcggtc cgcatgaggg cgatggaggc gcgcgggctc gccccgagcg cgatcccttc
     4321 ggcggcgcgg gtggcggcgg taagatcgac gatgtagcgt ttcatctctt cgctgatggc
     4381 gacgcttcgg accgcgcgga ggaccgccgt cacctcggcg gcgtcgctga ccggtcgcac
     4441 ctgcttgagc gggtgctcga taccctgcgc cgacatgacc gccacctcct cgtcgcgcgc
     4501 gatgtagccc atcgagagcc gcatcatgaa ccggtccatt tgcgattcgg ggagcgggta
     4561 ggtgccgcgc gactcgatgt tgttctgcgt cgcgatgacg aagaacggct cggggagcgg
     4621 ccgccggacg ccgtcggtgc tcacctgccc ttcggccatc gcctcgagga gcgccgactg
     4681 ggtgcggggg ctcgcgcggt ttatctcgtc ggtgagcagg atgttggtga atacgggccc
     4741 ttcgcggaac tcgaatttgc cgtcgtgctg gttatagacc gatacgccga gtatgtcgga
     4801 ggggagcagg tcgggggtga actggatgcg gcggaacggg atgtctatcg agcgggcgag
     4861 cgtcttggcg agcgtcgtct tgccggtgcc ggggtagtcc tccagcagga catggccgcc
     4921 gctgaaaaag gcggcg
//
LOCUS       DAOQUE010000185         4927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_18978_length_4927_cov_3.856527, whole genome shotgun sequence.
ACCESSION   DAOQUE010000185 DAOQUE010000000
VERSION     DAOQUE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18978_length_4927_cov_3.856527"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PKH10_06760"
     CDS             <1..714
                     /locus_tag="PKH10_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNT27860.1"
                     /translation="HPMTFAMVGMAGFFTAAAKTPISTVIMVSELTGNYELLLPSMWV
                     CAFAFLLTHGRTIYRSQIPSRALTLPHLGAVSTEKIDALAVAEVFHRTRRFVRISRHH
                     TLHEIMALTENSRQRIFPVVDDAGVLLGSFRIGQFFHAVQEDDPSTQTADDLLDDRAF
                     FIYENDSIGHALKILRNNDLEEILVVPADGSRQVLGILTTADILLAYHRNITPKPAQP
                     TGPAAPPANPDDRDTSAPS"
     gene            792..1565
                     /locus_tag="PKH10_06765"
     CDS             792..1565
                     /locus_tag="PKH10_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27861.1"
                     /translation="MKKILALFILSAAWSLGAAERPVFYFFHSENCPHCHEAKPFMAE
                     LKKKYPDIEFRELEVSRDLVNREILLKKAQQLGISRIGVPLFIIGKTYISGFKKGDFE
                     KKITDLIDAQLTGSSIKVTTPPSGLTIMQKEAIDIDEAIGRCYAQGPAAIPDCLKKGK
                     KRLEDLSLVIAGQVTAILEKMMIDGRMVPQKAAYLDQQALWKKQVEAEAAIDEAFCRA
                     HNATNCEGLALYLTVQHLQGRVQKLLGDLQSAQSLPAGR"
     gene            complement(1541..2305)
                     /locus_tag="PKH10_06770"
     CDS             complement(1541..2305)
                     /locus_tag="PKH10_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNT27862.1"
                     /translation="MEHSTGRTKKMGQKENGGDLDAATRRALTAHLGSFVTDHKRDLI
                     EHALAERTDHLALALEDIYQPHNASACLRTCECFGVQNIHIIEGRNRYEVNPDVVMGS
                     VKWLTLHRYREPDRDNTAVCISRLRELGYQVIATSPRADGYTPDDLPLDRPAAILFGT
                     EEMGLSTGALADADLSLRIPMYGFTESLNISVSVAVALSHLMRRLRASDVSWRLSEER
                     REALRLAWYRKVVSRSAAIEDAFLAAYGGQRPAGRL"
     gene            2418..3452
                     /locus_tag="PKH10_06775"
     CDS             2418..3452
                     /locus_tag="PKH10_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HNT27863.1"
                     /translation="MRRSFGSDNHAGVHPAVFDALRRVNDGDVHAYGHDAYTVDVARR
                     VASFFGPQAETFFVFNGTAANVISIRTACHPHQGVICAETAHINTDECGAPEWNAGVK
                     LLTVPTPDGKLTPALVERRLGGRGDEHRIQPRLVSISQTTEVGTCYTPAELETLSVFC
                     HGHGLYLHIDGARLSNAAATLNSTLGEAAAGADLISFGGTKNGLMAGEAVIVTDPALA
                     TGMLFLRKQSMQLSSKMRYLAAQFEAFLTDDLWLANARHANAMAQLLSKGIVRFPDLE
                     LAWPTQANGVFVRMPAELVEPLQKEYFFYLWDETVPIARLMCHFNTTEKDVRGFLAVV
                     ERLSGARTVR"
     gene            complement(3427..4896)
                     /locus_tag="PKH10_06780"
     CDS             complement(3427..4896)
                     /locus_tag="PKH10_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNT27864.1"
                     /translation="MNIPAVVSVLAAIVGIAVSFMILPLFVAILYGETLACHVFAWVM
                     SGGLFLCGATYVAMRHLARYDKRPRGLSPRDGFVTVTLSWVFVSLFGALPFLFSGTIP
                     SLADAYFETMSGFTTTGASILTDIESLPRSLLFWRALTQWLGGMGIVVLVVAVIPLLG
                     IGGVQLLEAEAPGPSVDKVAPRIKDTAKLLWLLYLGFTVLETVLLWVGGMSPYDALTH
                     TFTTLSTGGFSVRNASLGAYPGAYLQWVVTIFMLIGGTSFSIFFLLYRGRLGDIWRNS
                     EWRAYLLIFLLTTLFIVVELSTRGIYDSFGDDVRHAAFQVASIMTTTGFATADFSLWP
                     HFSQMVIFFLMFVGGCAGSTGGGIKVIRLVALFKLSVNEMRYLLHPRGVFTLTLDGRP
                     VRKDLMYGIVGFFFLYVFFVLITTLVVATANYDLSTSLTTALATVGNIGPGLSLIGPM
                     ANYAHFPDYVVWCLSFAMMAGRLEIYTVFVIFSGLFWRR"
BASE COUNT         1084 a         1543 c         1448 g          852 t
ORIGIN      
        1 catccgatga ccttcgcgat ggtcgggatg gcgggattct tcaccgcagc ggcaaagacc
       61 cccatatcga ccgtgatcat ggtgagcgaa ctgaccggga actacgaact gcttctgccc
      121 agcatgtggg tctgcgcctt cgcgttcctg ctgacgcacg gccggaccat ctaccggagc
      181 cagataccct cgcgggcact gaccctgccg cacctcggcg cggtctccac cgagaagatc
      241 gacgctctcg cggtcgccga ggtctttcac cgcacccgcc gtttcgtgcg gatatcgcgg
      301 caccacaccc ttcatgagat catggcgctc acggagaatt cccgtcagcg gatattcccg
      361 gtcgtggacg acgcgggcgt gttgctcggc tcgttccgca tcgggcagtt ctttcacgcc
      421 gtgcaggaag acgatccctc gacccaaacg gcggacgacc tgctcgacga ccgcgccttt
      481 ttcatctatg agaacgattc gatagggcac gcgctcaaga tattgcgcaa caacgatctc
      541 gaggagatcc tggtcgttcc cgccgacgga tcgcggcaag tgctggggat actgacgacc
      601 gccgatatac tgctggccta ccatcggaac ataacgccta aaccggcgca gccgaccgga
      661 ccggccgcgc caccggcgaa ccccgacgat cgcgacacct ctgctccatc ctagactcgg
      721 cacaaacttg acaaaaagcg gtccccttcc ttaggatgtg ccgtgtccaa gaactcttca
      781 tggaggggga tgtgaaaaag atccttgctt tgttcatcct ttcggccgcg tggtccctgg
      841 gcgccgccga acggccggtg ttctacttct tccattcaga gaactgcccc cactgccatg
      901 aggcgaagcc cttcatggcc gagctgaaga agaaataccc cgacatcgaa ttccgggaac
      961 tggaggtatc gcgcgacctc gtcaaccgcg agatactgct gaaaaaggcc cagcaactcg
     1021 gtatatcgcg catcggtgtt ccgcttttca tcatcggcaa gacctatatt tccggtttca
     1081 agaaaggcga tttcgaaaaa aagataaccg atctcatcga cgcacagttg accggctcat
     1141 cgatcaaggt caccactccc ccatccggtt tgaccatcat gcaaaaggag gcgatcgaca
     1201 tcgatgaagc gataggccgc tgctacgcgc agggtcccgc cgcgatcccc gactgtctca
     1261 aaaaaggaaa aaaacggctg gaagacctct cgctcgtcat cgcgggacag gtgacggcga
     1321 tactcgaaaa gatgatgatc gacggcagga tggtcccgca gaaggcggcc taccttgatc
     1381 agcaggcgct ctggaagaag caggtggagg ccgaagcggc catcgacgag gccttctgcc
     1441 gggcccataa tgcaacgaac tgcgaggggc tcgcgctcta tctgaccgtc caacacctgc
     1501 agggccgggt ccagaaactg ctcggcgatc tgcagtccgc tcagagccta ccggccggac
     1561 gctgaccgcc gtaggcggcg agaaaggcat cctctatcgc ggcgcttctc gacaccacct
     1621 tccggtacca tgccagccgc aatgcctctc gacgctcttc gctgagccgc cacgagacat
     1681 ctgaagcacg taaccggcgc atgaggtgcg aaagagcgac cgcgaccgac accgatatgt
     1741 tgagactttc ggtgaagccg tacatcggta tcctgagcga caggtccgca tccgcaagtg
     1801 cccccgtcga cagccccatc tcctcggtcc cgaacagtat cgccgcgggg cgatcgagcg
     1861 gcaggtcatc cggcgtatag ccatcggcac gcggcgaagt ggcgatcacc tgatatccga
     1921 gttcccgcag gcgtgagata cagacggcgg tgttgtcgcg atccggttcg cgataccggt
     1981 ggagcgtaag ccatttgacc gaccccatca ccacatcggg attcacctcg tagcggttcc
     2041 gcccctcgat gatatggatg ttctgcacgc cgaaacattc acaggtccgc agacaggccg
     2101 aggcgttatg gggctggtag atgtcctcga gcgcgagcgc cagatggtcg gtccgctctg
     2161 caagtgcgtg ctctatcagg tcgcgtttat ggtcggtcac gaacgaaccg aggtgcgcgg
     2221 tcagcgcccg gcgcgtcgcg gcgtcgagat cgcctccgtt ctctttctgt cccatcttct
     2281 tcgttctccc tgtcgaatgt tccatcctac cgcgtcacgc cccgtttggc aagaaagtgc
     2341 cgtagaacag accaacgggt tgcttttttt ctcccgaagg catacgatga cgctgtaaaa
     2401 ggccagcgga ggatgccatg cgacgcagtt tcggttcgga caaccacgcc ggggtccacc
     2461 ccgccgtttt cgatgccttg cggcgcgtga acgatggcga tgtccacgcc tacggccacg
     2521 atgcatacac cgtcgatgtc gcccgaaggg tcgcgtcctt tttcggcccg caggcggaga
     2581 cattcttcgt cttcaacggc accgccgcga atgtcatctc gatacggacc gcctgccatc
     2641 cccatcaagg cgtcatctgt gccgaaacag cccacatcaa caccgacgag tgcggcgcac
     2701 cggaatggaa cgccggggtg aaactgctca ccgtcccgac ccccgacggc aaactgaccc
     2761 ccgcccttgt cgagcggcgt ctcggcggcc gcggcgacga gcaccgcatc cagccacgcc
     2821 tcgtttcgat atctcagacg accgaggtgg gcacctgtta tacgccggcc gagcttgaaa
     2881 cgctcagcgt cttctgtcat ggtcatggcc tctaccttca catcgatggc gcgcggctct
     2941 ccaacgcggc ggcgacgctg aactcgaccc tcggcgaggc ggcggcggga gccgatctta
     3001 tctccttcgg cggcaccaag aacggactca tggcgggcga ggcggtcatc gtcaccgacc
     3061 cggcgctcgc gaccgggatg ctcttcctcc gcaaacaatc gatgcagctt tcttcaaaaa
     3121 tgcgttatct cgcggcgcag ttcgaagcat ttctgaccga tgatctctgg ctcgcgaacg
     3181 cccgtcacgc caacgcgatg gcgcaactgc tttcaaaggg gatcgtgcgc ttcccggacc
     3241 tcgaactcgc gtggccgaca caggcgaacg gcgtattcgt caggatgccc gccgaactcg
     3301 tcgaacccct gcagaaagaa tattttttct atctctggga cgaaacggtc ccgatagcgc
     3361 ggctcatgtg ccacttcaac acgaccgaga aggacgtgcg gggattcctt gcggtcgtgg
     3421 aacggcttag cggcgccaga acagtccgct gaagataaca aagacggtat atatctcgag
     3481 acgtcccgcc atcatcgcga acgacagaca ccacacaaca taatcgggga agtgggcgta
     3541 gttggccatc ggccctatca gcgagagacc gggaccgatg ttgccgacgg tcgcaagggc
     3601 ggtggtaagc gaggtagaaa gatcgtagtt cgccgtcgcg accacgaggg tcgttatgag
     3661 gacgaaaaac acatagagga aaaagaagcc gacgatgccg tacatgaggt ccttgcgcac
     3721 cggtcgaccg tcaagcgtca gggtgaacac gccgcgcgga tggagtaggt agcgcatctc
     3781 gttgacggag agtttgaaaa gcgccacaag gcggatgacc tttatgccgc cgccggtcga
     3841 tccggcacaa ccaccgacga acatgaggaa gaagatgacc atctgtgaaa aatggggcca
     3901 gagcgagaag tcggcggtcg caaaccctgt cgtcgtcatg atggaagcga cctgaaaggc
     3961 ggcgtgacgg acatcgtcgc cgaacgagtc gtatatgccg cgggtcgaca gttctaccac
     4021 gatgaacagg gtggtcagca aaaagatcaa caggtaggcc cgccattcac tgttgcgcca
     4081 tatatccccc agacgccccc ggtataacag gaagaatatc gagaaactgg tgccgccgat
     4141 aagcatgaag atcgtcacca cccactgcag ataggcaccg ggataggcgc cgagcgaggc
     4201 gttgcgcacc gagaacccac cggtcgaaag agtggtgaag gtgtgggtca gcgcatcgta
     4261 gggactcatg ccgccgaccc agagcaacac cgtctccaag accgtgaagc cgaggtacag
     4321 gagccacagg agtttcgccg tatccttgat gcggggagcg accttatcga ccgaaggacc
     4381 gggcgcctcg gcctcgagaa gttgtacgcc gccgatgccg aggagcggga taacggcgac
     4441 caccagcacg acgataccca tgccccccag ccactgggta agggcccgcc agaagagcaa
     4501 ggatcgaggg agcgactcga tgtcggtcag gatcgaggca ccggtcgtcg tgaacccgga
     4561 catggtctcg aagtaggcgt cggccagcga tggtatcgtc cccgagaaca gaaaaggtaa
     4621 tgcgccgaac agcgaaacga acacccacga gagcgtcacg gtcacgaacc catcgcgcgg
     4681 cgaaagtccg cgggggcgct tgtcgtaccg ggccaagtga cgcatcgcga cataggtcgc
     4741 gccacacagg aacagccccc ccgacatgac ccaggcgaag acatgacacg cgagggtctc
     4801 gccgtagagg atcgcgacga aaaggggcag tatcatgaag gaaacggcga tcccgacgat
     4861 cgccgccagc acgctgacga cggccggtat gttcatgccg ctccgccgaa aagcgtttcc
     4921 atccgcg
//
LOCUS       DAOQUE010000186         4908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_19084_length_4908_cov_4.130229, whole genome shotgun sequence.
ACCESSION   DAOQUE010000186 DAOQUE010000000
VERSION     DAOQUE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19084_length_4908_cov_4.130229"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKH10_06785"
     CDS             <1..275
                     /locus_tag="PKH10_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HNT27865.1"
                     /translation="RKGEKSFHFIEIMGCPGGCVGGGGQPIPTSWKIKAERAAAIYKE
                     DDSREMRKSHVNPGITQIYKEFLEKPNSHKAHELLHTHYHARDNIR"
     gene            423..2396
                     /locus_tag="PKH10_06790"
     CDS             423..2396
                     /locus_tag="PKH10_06790"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT27866.1"
                     /translation="MIDLERRHIIREQWARFWNGEAVDTAVIHPYLLASWERSRALGV
                     PRHIERLPVASSGELINRIHESRRMLGVAVPYMKKIISFMNDPESIIMITNGQGIVLE
                     LFGSPEMLARDTAPKLGSRLREEDIGTNGVGTALIEDRTLDIWAGEHYLEALHYWYAS
                     ADTIHDKEGKILGCLCLICRWDLVNAHSLGLVGAVAGAIERQLHIEDALAEKTVAMQK
                     QQAIVGSIAEGIVVIDGDEKIREINPSARRMLGLGPEDHAGRSIYTVIPDGINFFKIM
                     ADGANIHDREAVLSLRDRRKLHCAVSAALIQSEGAVGGMVITLKEAKSLHQLAGKIAG
                     SRAYLSFTDIIGTSQALQEAVKLARTAAKSRSNVLLLGESGTGKELFAQSIHNFSDRA
                     PHSFVAVNCGALPRDLVESELFGYEGGAFTGSKKEGRPGKFELADEGTIFLDEIGDMP
                     LEAQVNLLRVLQSSEVVRVGGTYPKKIDVRVIAATNHDLKKAVEQKAFRDDLYYRLNV
                     LAITIPPLRERVDDIEALALHFAKRSAHAMNKPTPAVTPEAMRLLKSYPWPGNIRELE
                     NVLERAVNIMERPEITPVELPDHLRAAGTERSPLPEGPSLKAREYETILRMLEETGGN
                     LRAASARLGIARGTLYNKVVKYRIDLEKFRSNG"
     gene            2600..4507
                     /locus_tag="PKH10_06795"
     CDS             2600..4507
                     /locus_tag="PKH10_06795"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNT27867.1"
                     /translation="MRLLTKFILTTVAIFLMLAIALSAVSTLWINQNTIRDAQHRVEV
                     AIRWAREAFNQKNRDAHQVLAVAAQNELLRDFLDNPRDPKLAAAVQEKFNNFRLQYHM
                     DLLNIVSADGTVLLRTRPPFNSGDDISDNPFIRHVLLNGKSIEGTIVLSRSALAREGE
                     SLLERAVDATGEPNGMFTGTAVPVMRGGKLIGIISSGILINGNNDFIDTIRDAVSYGD
                     YYKGRSVGTATIFMGDKRIATNVVGNKAGRATGTAASAEVADMVLLRGRSWSGRAWVV
                     DAWYLSYYEPLRDPDNDLIGMLYVGELEEKYRDIRFKAVASVVSVIFVAMLLSIAMFI
                     LIARSILEPVGKLRDATARLIKGERSARVTEEFGGEMGDLTRSFNNMADDIEKHTAEI
                     ERKQCELAALNNELEMTNRNYIDMLGFISHELKNPLASAVLSLHTVKDGYLGELNDKQ
                     KKGLDNVAESLDYFKELIRSYLDLSKLEKGDYVINRRNTALKNAVIDPAIQGLTEEIR
                     RHHMTLDIGVHDDTVVDADPDLLRIVYDNLIGNAVKYGREGGAIRLREKVRDGRQVFS
                     VYNEGAGIPREKMDMLFKKFSRIYSQDTAGKRGSGLGLYACREIVRKHGGDIWAESEP
                     GKWVEFSFEIG"
     gene            4520..>4908
                     /locus_tag="PKH10_06800"
     CDS             4520..>4908
                     /locus_tag="PKH10_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT27868.1"
                     /translation="MTRKILIADDDVQMVSALRTLLENVGFAVTVTHHSKEVLELTRR
                     ERPDLILLDVLFAGMPGPDGFEVSRKIAKDAELRTTPVIILSGVKRVIASDFTVEPDE
                     EWLPVAAFLDKPVRPDQLLKEIERLLS"
BASE COUNT         1049 a         1482 c         1510 g          867 t
ORIGIN      
        1 tccgtaaggg cgagaagtcg ttccacttca tcgagatcat gggctgtccc ggcggttgcg
       61 tcggcggcgg cggccagccg atcccgacat cgtggaagat caaggcggaa cgcgcggcgg
      121 ccatctacaa agaggacgac agccgcgaga tgcgcaagtc gcatgtcaac cccggcatca
      181 cccagatcta caaggagttc ctcgagaagc cgaacagcca caaggcgcac gaactcctcc
      241 acacccatta ccacgcccgc gacaacatcc gctagtattc agtttttcgt atttacaggg
      301 gcggcccgct gtggccgccc tttttttgtc gcgcacatgc ggcattttcg gaaggatttc
      361 ttgcttcgcc gcccgggaac ggataggatg ggcggcgtga ggaccgtgat gaatagggac
      421 gcatgataga tctcgaacgc cgccatatca tccgcgaaca gtgggcgcgc ttctggaacg
      481 gtgaagcggt cgacaccgcc gtcatccatc cgtacctgct cgcctcgtgg gaacggagcc
      541 gcgcgctggg tgtgccgcgc catatcgaac ggctgccggt cgcctcgtcg ggcgaactga
      601 tcaaccgcat ccacgaaagc cgccggatgc tcggcgtcgc ggtcccctac atgaagaaga
      661 tcatctcctt catgaacgac cccgagagca tcatcatgat caccaacggg caggggatcg
      721 tgctggaact gttcgggtcg ccggagatgc tggcgcgcga cacggcgccg aaactgggga
      781 gccgcctgcg cgaagaggat atcgggacca acggcgtcgg cacggcgctc atcgaggacc
      841 ggacgctcga tatctgggcg ggggaacact atctcgaggc gctccactac tggtacgcga
      901 gcgccgacac catccacgac aaggagggga agatactcgg ctgtctctgc ctcatctgcc
      961 ggtgggatct cgtcaacgcc cattcgctgg ggctcgtcgg cgcggtcgcc ggggcgatag
     1021 agcggcagct gcacatcgag gatgcgctgg ccgaaaagac cgtggcgatg cagaaacagc
     1081 aggcgatcgt cgggtccatc gccgaaggga tcgtggtcat cgacggcgac gagaagatac
     1141 gggagatcaa tccgtcggcg cggcggatgc tcgggctcgg gccggaggat catgccggcc
     1201 ggtcgatata caccgtcatc cccgacggca tcaatttctt caagatcatg gccgacggcg
     1261 ccaatataca cgaccgcgag gcggtgctgt cgctgcgcga ccgacggaaa ctccactgcg
     1321 ctgtgtcggc cgcgctgata cagagcgaag gggcggtcgg cggcatggtc atcacgctca
     1381 aggaggcgaa atcgctccat cagctggcgg gcaagatcgc cggttcgcgc gcctatctgt
     1441 ccttcaccga catcatcggc acctcgcagg cgctgcagga ggcggtgaaa ctggcccgga
     1501 cggcggccaa gagccgctcc aatgtgctcc tcctcggcga aagcggcacc ggcaaggaac
     1561 ttttcgccca gtcgatacac aatttcagcg accgcgcgcc tcattcattc gtcgcggtca
     1621 actgcggcgc attgccgcgc gatctggtgg agagcgaact gttcggctac gaagggggcg
     1681 ccttcaccgg ttcgaagaaa gaggggaggc cggggaagtt cgaactggcc gacgagggga
     1741 ccatcttcct cgacgagatc ggcgatatgc cgctcgaggc gcaggtcaac ctcctgcggg
     1801 tcctccagag ttccgaagtg gtccgtgtgg ggggcaccta tcccaaaaag atagatgtcc
     1861 gcgtcatcgc cgccaccaac cacgatctca aaaaggcggt cgaacagaag gcgttccgcg
     1921 acgatctcta ctaccggctc aatgtcctcg ccatcaccat tccgccgttg cgcgaacggg
     1981 tggatgacat agaggcgctc gcgctccatt tcgccaagcg ctcggcccac gccatgaata
     2041 agccgacccc cgccgtcacc cccgaggcga tgcgtctgct caagtcctat ccgtggcccg
     2101 gcaacatccg cgaactggag aatgtattgg aacgggcggt caacatcatg gagcgccccg
     2161 agatcacccc cgtcgagctg cccgatcacc tgcgtgccgc cggtaccgaa cggtctccgc
     2221 ttcccgaagg tccctcgctc aaggcgcggg agtacgagac gatactccgg atgctcgaag
     2281 agaccggcgg taatcttcgc gccgcttcag cacggctggg catcgcgcgc ggcaccctct
     2341 ataataaggt ggtcaagtac cggatcgatc tcgaaaagtt ccggagcaac ggctgatctc
     2401 aaagcgttca atttttgaac atgctcaatt gaacatgtgt atattggata caatatgaag
     2461 atgggcgctt tttagagaaa gtccccgctc ggaaaaaata taatccaata gttcaatata
     2521 gttacaaaac aaaaatcttt gttttggtct ttggcatggc ttttgtgcta tgtatcatgt
     2581 tttttgacga ggtgatgtga tgcggctcct gaccaagttc atcctgacaa cggtggcgat
     2641 cttcctgatg ctcgccatcg ccctctcggc ggtcagtacc ctctggataa atcagaatac
     2701 gatacgcgac gctcagcacc gggtcgaagt ggcgatccgc tgggcgcgcg aggcattcaa
     2761 ccagaagaac cgcgacgccc atcaggtgct cgcggtggcg gcgcagaatg aactgctccg
     2821 cgatttcctc gataaccccc gcgacccgaa actggccgcc gcggtgcagg agaagttcaa
     2881 caacttccgg ctccagtatc acatggacct tctcaacatc gtgtcggccg acggcacggt
     2941 gctcttgcgg acgcggccgc ccttcaatag cggcgacgat atctccgaca atccctttat
     3001 ccgtcatgtg cttctgaacg ggaagagcat cgaggggacg atcgtgctgt cgcggtcggc
     3061 gctggcgcgc gaaggggaga gtctgctcga acgggcggtc gacgcgaccg gcgagcccaa
     3121 cgggatgttc accggcaccg cggtcccggt gatgcggggc gggaaactca tcgggatcat
     3181 ctcgtcgggt atcctcatca acggcaacaa cgacttcata gacaccatcc gcgacgcggt
     3241 atcctacggc gactactaca aagggcgttc ggtcggcacc gcgacgatct tcatgggcga
     3301 caagcggatc gccaccaatg tcgtcggcaa caaggcgggc cgggcgaccg gcaccgcggc
     3361 gtcggccgag gtcgccgata tggtgctcct gcgcggccgg tcgtggtcgg ggcgggcgtg
     3421 ggtggtcgat gcgtggtacc tctcctacta tgaaccactc cgcgaccccg acaacgacct
     3481 catcggcatg ctctatgtcg gggaattgga ggaaaaatac cgcgacatcc gcttcaaggc
     3541 ggtggcgagc gtggtgtcgg tcatcttcgt cgcgatgctg ctcagtatcg ccatgttcat
     3601 cctgatcgcg cgctccatcc tcgaaccggt cgggaaactg cgcgacgcca cggcgcgcct
     3661 gataaaagga gagcggtcgg cgcgggtgac cgaggaattc ggcggcgaga tgggcgatct
     3721 gacccggtcg ttcaacaaca tggccgacga catcgagaag cacaccgccg agatcgaacg
     3781 gaaacagtgc gaactggccg cgctcaataa cgaactcgag atgaccaacc gcaactacat
     3841 cgatatgctc ggcttcatca gccacgaact gaaaaacccg ctcgcgagcg cggtcctgag
     3901 cctccatacc gttaaggacg gctatctggg cgaactcaac gataaacaga agaaagggct
     3961 ggacaacgtc gccgagagtc tcgactactt caaggaactg atccgcagtt atctcgatct
     4021 ttccaaactc gagaagggcg actatgtcat caaccgccgc aatacggcgc tcaagaacgc
     4081 ggtgatcgac ccggcgatac agggactgac cgaagagatc cgccggcacc acatgacgct
     4141 cgatatcggc gtccacgacg acacggtggt cgacgccgac cccgatctgc tccggatcgt
     4201 ctacgacaat ctgatcggta acgcggtcaa atatggccgc gagggcggcg cgatccgtct
     4261 gcgcgaaaag gtgcgggacg gccggcaggt attcagcgtc tacaacgaag gggcgggaat
     4321 accgcgcgag aagatggata tgcttttcaa gaaattctcg cgcatctatt cgcaggatac
     4381 cgccgggaaa cgcggttcgg ggctggggct gtacgcctgc cgcgagatcg tccgcaagca
     4441 cggcggcgat atatgggccg aatcggagcc cggcaagtgg gtcgagttca gtttcgagat
     4501 aggataagag gtgagagata tgactagaaa gatccttatc gccgacgacg atgtccagat
     4561 ggtcagcgcg ctgcgcaccc tgctggagaa tgtcggattc gccgtcacgg tgacccacca
     4621 ttccaaagag gtgctggaac tgacccggcg cgagaggccc gacctgatac tgctcgatgt
     4681 cctgttcgcc gggatgccgg gccccgacgg gttcgaggtg tcgcggaaga tcgccaagga
     4741 cgccgaattg cggaccaccc cggtcatcat tctgagcggc gtgaaacggg tcatcgcgag
     4801 cgatttcacg gtggagcccg acgaggagtg gctgccggtc gccgcgttcc tcgacaaacc
     4861 ggtccgcccc gatcaactgc tcaaagagat cgaacggtta ctttccga
//
LOCUS       DAOQUE010000187         4902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_19108_length_4902_cov_4.281205, whole genome shotgun sequence.
ACCESSION   DAOQUE010000187 DAOQUE010000000
VERSION     DAOQUE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19108_length_4902_cov_4.281205"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKH10_06805"
     CDS             <1..431
                     /locus_tag="PKH10_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27869.1"
                     /translation="KKYWPIAITVMLSFFLLIGFQHHYFSPLHETGGNSHLAFRTGER
                     ELKQSAYFHVLKESGGAPIRIFADGWWLWQPFLYFATNNSSVSILELREFSKPVKGDY
                     VVTFAGERLDLAIQKEPDSYEKRWTELDYAGRSILAIYRL"
     gene            complement(411..2642)
                     /locus_tag="PKH10_06810"
     CDS             complement(411..2642)
                     /locus_tag="PKH10_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27870.1"
                     /translation="MFGAGLWDGGIISPGDTDIFLEFYHMRGFTARELLAGNIPLWNP
                     HIYSGAPFLMDPETGIFYPLNWVFLFLPTPLAINTSVILHLTLLGTLTSLWIRRLGND
                     PVVSLMAGSLAMFGGAYFMHTYAGHLGILSTAAWTPLLLLSLDRNAEEWRKNWFFAGL
                     FAAGMSFLAGHLQYVIISATAAALYALFLAAERRQVRTLWCVLCWYGGGVFLGAAQAF
                     PAVDGLSESYRSALTDSYLASFSFHPENLLTLIAPDFFGNMDTVPYWGKEYLWEQSIF
                     LGVGSLFLIIYGIRDRSGKLLALAAFLVLLIALGAHTPLFAVTSRYVPFLNKLRGHGK
                     FIFGATILLVPLMARGAFRLREERPSWLTVGLVAGSALVAGGAAYAVGNAASLEEWRA
                     LMWSLGPGDGRFLWYGDPAIFNDAAFIERAQGSAAVSLYGVAGKLLLIALFVAAMRYR
                     REAFYAILCLVVLDLLFFARSYGRTFPQEQMRSPDLEAPYVRELGDDRIYSDFAPNRV
                     MIYGLYDANGYNTKVALYRYKRFSDTFFVPSYWTLLSDGSVVGPARIADLLRLRYLNA
                     WERGGVTRYKVRGEPLPRVLLVDRFEVHRREEDIFKVLTNTSFDPRHSVILEKDPAIK
                     PLADGMTGVVKVVGGDTDTLIIEAELSSPRILLVTDGYSRHWRATPLEGSAQSDYEVM
                     PADYIFRAVPLAAGSHRLLLEYAPLSFTIGAWTAFLSWSLLFVLAFLYPLSRSGRSKA
                     VDG"
     gene            2832..3794
                     /locus_tag="PKH10_06815"
     CDS             2832..3794
                     /locus_tag="PKH10_06815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT27871.1"
                     /translation="MMKISVIVPCYNEQEVLRETCRRIKDVLASNGLTDHEMLFVNDG
                     STDGTPLLLREFAERDRAVKVIHFSRNFGHEAATAAGIQHCSGDIAFLLDADLQDPPE
                     LIPEMIALWRKEKSNVVYGVRKSREGETFLKKLTSKLFYRFFNAISDVPFPIDTGDFR
                     LIDHKVIAEFKRLGERKMYVRGLLTWVGFKQIPFFYERQKRFAGTTKYNYRKLLRLSF
                     DIIFSFTKRPLKLALELGAASVIISLLLLLYAFVSRFIAPLPGWASTIIIIIFFGGVQ
                     LLTIGVIGEYIAIIFDEVKRRPQYIIDELMNLDAPVDKDRSNRR"
     gene            complement(3943..>4902)
                     /locus_tag="PKH10_06820"
     CDS             complement(3943..>4902)
                     /locus_tag="PKH10_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27872.1"
                     /translation="PLHEWSSIFSLGPLRYSQYYILFYLFSALMLWAVWESRRTRPFY
                     ALVTLLLLVNAYLHARFAADVALVGGVLAVPLIAERMPGTPVRPLLYALVFAVAMALG
                     VRSMQQLNYHWGVGIDPDKVPLHAGEFIRDHDLAGNFFGIYSGSTDFLMAYAYPRVKV
                     AVDIRVPGLYPYEFASRYWNVTNEKDLRDHVLSLPVDYIVLGRPDILSYQTHEIHVER
                     ILLSEGWALYHFDDRYALYGSPRIRDTVALMPFRIISRWNSDVREIGAAVAAGRFDEL
                     AGELERLKKYTAERGGFYREVLMTLYRQPFLGNEQKELLEEMF"
BASE COUNT         1252 a         1428 c         1300 g          922 t
ORIGIN      
        1 caaaaaaata ctggccgatc gccattaccg tcatgctttc cttttttctc ttgattggtt
       61 tccagcatca ttatttctct cccctccatg aaaccggagg caactcccac ctcgcattca
      121 gaacagggga aagagaactt aaacaatcag cctacttcca tgtgcttaaa gagagcggcg
      181 gcgctccgat aagaatattc gctgacgggt ggtggctctg gcaaccattc ctgtattttg
      241 ccacgaacaa ctcttcggtt agcatattag agttgcgaga gttcagtaaa cccgttaaag
      301 gtgactatgt tgttactttt gccggggaac gacttgatct ggcgatacag aaagaacctg
      361 actcctatga aaagcgctgg acggagctcg attatgccgg aagatcgatt ctagccatct
      421 accgcctttg agcgaccgga ccgggacaac ggatacagaa aagcaagcac gaaaagcaac
      481 gaccaagaaa gaaaagcggt ccacgcaccg atggtgaacg aaagcggagc atattcaagc
      541 aggagacgat ggctcccggc cgcgagcggt accgcgcgga agatatagtc ggccggcatg
      601 acctcgtagt cgctctgcgc gcttccctcc agcggggtcg cccgccagtg ccgactgtat
      661 ccgtcggtca ccagcagtat ccgcggagag gaaagttcgg cctcgatgat cagcgtatcg
      721 gtatcgccac ccaccacttt taccacacca gtcatcccat cggcgagcgg ttttatcgca
      781 ggatcttttt ccaaaataac gctatgcctt ggatcaaatg aggtgttggt gagcacctta
      841 aagatatcct cctcccgcct gtgaacttcg aaacgatcca ccagcagcac acggggtagc
      901 ggttctcctc gtaccttgta gcgcgtgaca cctcccctct cccatgcatt cagataccga
      961 agcctaagca gatcggcgat ccgagccggt cccaccacgg aaccatcaga caatagagtc
     1021 cagtaggatg gaacaaaaaa ggtgtccgaa aatctcttgt aacgatacag cgccaccttg
     1081 gtattgtacc cattggcatc gtacagacca tagatcatca cccgattcgg cgcaaaatca
     1141 ctgtatatcc tgtcatcgcc gagttctcgg acatatggcg cctccagatc gggagaacgc
     1201 atctgttcct gcggaaaggt ccggccatag gagcgggcga agaacagaag gtccaacacc
     1261 acaagacaca ggatcgcgta gaaagcttct ctacgataac gcatcgcggc aacgaacagg
     1321 gcgatcagaa gcagttttcc ggcgacgccg taaagcgaca ctgccgcgga tccctgcgcc
     1381 cgctcgatga acgccgcatc attaaatatc gccgggtcac cgtaccacaa gaaccgaccg
     1441 tcgccgggac ccagggacca catgagggcg cgccattcct caagcgaagc ggcattgccg
     1501 accgcatacg ccgctccgcc ggcgaccaaa gctgaacccg cgacgagacc gacggtaagc
     1561 cacgagggac gctcttcccg gagtcggaag gcaccgcgcg ccataagcgg gacaagcaat
     1621 atcgtcgcgc cgaatatgaa cttgccgtgt ccacgtaatt tgttgaggaa cggcacatac
     1681 cgcgaggtga cggcgaacag tggagtatgc gctcccagcg cgatgagcag gacaaggaac
     1741 gccgccaacg ccaagagttt gccggaccgg tcgcgtatgc cgtagatgat cagaaaaaga
     1801 gagccaacac ccagaaagat cgactgctcc caaagatact ctttgcccca atacggcacc
     1861 gtatccatat tgccgaaaaa atctggggcg atgagagtaa gaagattttc gggatgaaac
     1921 gaaaaagagg caaggtacga atcggtcagt gcggagcgat aggattcgga aagaccgtca
     1981 accgccggga aggcctgcgc cgcccccaga aacacgccgc caccgtacca gcagagtaca
     2041 caccagaggg tccgaacctg tcgcctctcc gcagcgagga atagggcata cagcgcggcg
     2101 gccgtcgccg aaatgatgac atactgcagg tggccggcaa ggaaagacat acccgccgcg
     2161 aaaagaccgg caaagaacca gttctttcgc cactcctcgg cattccggtc aagtgacagg
     2221 agaagaagcg gggtccatgc ggcggtcgag aggataccga gatgcccggc ataggtgtgc
     2281 atgaaatagg caccaccgaa catcgcaagc gatcccgcca tgagcgagac cacagggtcg
     2341 ttccccagtc gccggatcca cagggaggtc agggttccca aaagcgtcaa gtgcaagatc
     2401 accgaggtat tgatcgccaa cggcgtcggc agaaaaagga atacccagtt gagcggataa
     2461 aatatgcccg tctccgggtc cataagaaat ggggcaccac tgtagatatg cggattccaa
     2521 agaggtatgt tcccggcgag caattcgcgc gcggtgaagc cccgcatgtg gtagaactcg
     2581 agaaaaatgt cggtgtcacc cggcgagatg atgccgccat cccagagtcc ggcgccaaac
     2641 accgcgcaag ccatcgtcgc gaaaatcacg accgtggccg atacgataag ggcgggaaaa
     2701 gcacattctc gcaaaaggtg atcctcggaa gtggcgtacc aattgtagat gaggcgaccg
     2761 gaaaagcaag gttaacttga ttttttggcc catttttcgc atacttccca tcataccgtg
     2821 tgaggagcac gatcatgaag atatcggtca ttgtaccctg ttacaacgaa caggaggtat
     2881 tgcgagagac ctgccgccgc atcaaggatg tgctggccag caatggcctg accgaccacg
     2941 agatgctctt cgtcaacgac ggaagcaccg acggcacccc ccttctgctc cgcgaattcg
     3001 ccgaacggga ccgtgcggtc aaggtgatac atttctcgcg caatttcggc cacgaggcgg
     3061 ccaccgccgc aggcatccag cactgctccg gggacatcgc cttcctgctc gacgccgatc
     3121 tgcaggaccc gccggaactc atccccgaga tgatcgccct ttggcgtaag gaaaaaagca
     3181 atgtcgtgta cggggtccgt aaaagtcgcg agggggagac ctttctcaaa aaactgacct
     3241 cgaaactctt ctatcgcttt tttaacgcca tttccgatgt ccccttcccc atcgacaccg
     3301 gcgacttccg actcatcgac cacaaggtca tcgccgaatt caaaagactc ggcgagcgga
     3361 agatgtatgt acgcggactt ctgacctggg tcgggttcaa gcagataccg ttcttctacg
     3421 agcgccagaa acggttcgcc gggacgacga aatacaacta ccgaaaactg ctccgactct
     3481 ccttcgacat cattttctcc ttcactaaac gaccactcaa actggcgctc gaactcggcg
     3541 ccgcgtcagt gatcatctcg ttgcttcttc tcctctatgc ctttgtcagc cgcttcatcg
     3601 caccgctccc cggctgggcc tcgaccatca tcatcattat ctttttcggc ggcgtacaac
     3661 ttctgactat cggcgtcatc ggtgaatata tcgccattat tttcgacgag gtgaaacggc
     3721 gaccgcagta catcatcgac gagttgatga atcttgacgc acctgtcgac aaggaccggt
     3781 cgaacagacg ataatgtcca tcttttgaac gagcaacagc ggttccccta ctggtggttc
     3841 cggagacgcg atcggatact taaccccatg attcctatcc atcaatatgg cctcagtctc
     3901 atcgctcatg cgcggaagtg aggcgccatt cattcggcgg gatcagaaca tctcttcgag
     3961 cagttctttt tgttcgttac cgaggaacgg ctgtcgatag agcgtcatca gcacttcgcg
     4021 ataaaaaccg cctctttcgg cggtatactt tttaagcctt tcaagttctc ccgccaactc
     4081 atcaaaccgc cccgccgcaa ctgccgcgcc gatctcgcgc acatcgctgt tccagcggct
     4141 gatgatgcgg aacggcatca atgcgaccgt atcgcggatg cggggagatc cgtacaaggc
     4201 ataccggtcg tcgaaatgat acagcgccca tccctccgag agcagtatcc gctccacatg
     4261 tatctcgtgg gtctgatacg aaaggatgtc ggggcgacca agcacgatgt aatcgaccgg
     4321 caacgaaagg acatggtccc gcagatcctt ttcattggtg acattccaat accgggaggc
     4381 gaactcgtac ggatagaggc ccggaacgcg gatgtcgacc gccaccttga cccgcggata
     4441 ggcataggcc atcaggaaat cggtggagcc cgaatagatg ccgaaaaaat tccccgcgag
     4501 atcatggtcg cgtatgaact cgcccgcatg caaggggacc ttgtcgggat ctatgccgac
     4561 accccaatga tagttgagtt gctgcatcga cctcacgccg agggccatcg caacggcaaa
     4621 gaccagcgcg tagaggagcg gtcgcaccgg cgtaccgggc atccgctccg cgatgagcgg
     4681 caccgccagt acaccaccca cgagcgcaac atcggcagcg aaacgggcat ggagatacgc
     4741 attgacgagc aggagcagtg tcacaagcgc ataaaaaggc ctggtacggc ggctttccca
     4801 taccgcccag agcatgagtg cgctgaaaag atagaacagt atataatact gactgtaccg
     4861 aagaggcccc agcgaaaaga tagaactcca ctcatggaga gg
//
LOCUS       DAOQUE010000188         4897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_19132_length_4897_cov_4.266212, whole genome shotgun sequence.
ACCESSION   DAOQUE010000188 DAOQUE010000000
VERSION     DAOQUE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19132_length_4897_cov_4.266212"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..1055
                     /locus_tag="PKH10_06825"
     CDS             312..1055
                     /locus_tag="PKH10_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT27873.1"
                     /translation="MSYLKSRGFRPAKNEPEEKNIAEKDAFQEQGQRLIDRLASHPYF
                     IIGTVAAVIVTVVIAILVADKWSESRSAKARSFVDAMALWEGKGTEGTAPKDETARLK
                     EALAKFAEAEKTLQGSFMGDTAAFYQAKIHYRLKEFDSAIKLFQRLQDSSKIPTDIRF
                     GAYEGEAYCHLDRDDPASAVAVWERYYALKNAPLYRDFALYYIGTAYEKMNDAAKAVE
                     YFKRLKAEFPESPLVSKVADRLPEDRKPS"
     gene            1066..2238
                     /locus_tag="PKH10_06830"
     CDS             1066..2238
                     /locus_tag="PKH10_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27874.1"
                     /translation="MIRIPLLAIFLLLVAASLGAEELVINWAVPLKNEFASIKKREFA
                     APLYRDGLIYVALRDGRLMVFGRDGRKAGERRFTGGFVVPPVEIAGGVLVCESHTAHL
                     LENGSLRDRWAVAGRSPVVARPISQPEGLYLQFLEHTVYLVDPADGAVKANYTFYGES
                     QLSYLMLSQPVAYEDKRVFGFSNGQVFFFLYRKDSYGVDEILPFYKYQTGEIQVGAEK
                     RDFYDIFSLLPGSEGMLLFSNGEAGGSIRVSNGAVTKTGELRNLTLKERADGTVLAYG
                     EAGALVLAKDGTVERRLFSSPSFVTGIVEAGSYALVTEMHGVVSLYLADLSRKLATVR
                     IPQGVSNGGVWGDGAFYLLSDMGTLYSFGVVKKEKDQKILEKSAEPDMKGDSSRTK"
     gene            2257..2532
                     /locus_tag="PKH10_06835"
     CDS             2257..2532
                     /locus_tag="PKH10_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT27875.1"
                     /translation="MNKSDLINVLTQRANLSKRNAEQFVNLMVDAMIDAMRKEERIEI
                     RGFGSFVIRHYDQYEGRNPRTGQKIHIAGKRLPFFKAGKDLLQRVNI"
     gene            2587..>4897
                     /locus_tag="PKH10_06840"
     CDS             2587..>4897
                     /locus_tag="PKH10_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNT27876.1"
                     /translation="MLRHYINSNDGNEILAVGSVNGDGFVHKVELFGMGTDGAVPAVL
                     KAARAGDVLIHNHPGGDIRPSDADIGIASEAAEKGVGSYIVDNGVTRIFPIVRWMPVR
                     DEEFHPVAPEEVAAVFAEKGRIAASYPEFEFRPPQSEMALAATEAVNGGSMLVVEAGT
                     GTGKSFSYLVPLLLYVAMNEGRKAVVSTSTIALEEQLSGKDIPFLQEHLGLSHIPVAV
                     LKGRQNYLCLRKYDLYRRGTLELPFDGRNGRSASLDDLDAWIGLPHDGSKSAMNTAIE
                     SEVWGEICCDEHSCDRAKCRFFSKCFFFRSRRQANFAQILLVNHHLLMADIALRDEEE
                     HAAGIIPPYDILVIDEAHNLFKAAVSFLGESASLSGILRSLKRLFSNDRGTGLLARLI
                     DAYAEPDTVRDLERLAGEIAAFVPFYIHSIVPECMERFKGAKENYLSLDDLAMRGEMR
                     EQFLRVTQFLGAFCERLGPVLDRIGTRLSDDPLSRSREEDMIASLLTETRGTLRRLEN
                     AKEFLDLFFTSADTDGAVFWGEKVSATNLRLSVTPLDIQRIFVRSIYEKVRSVLFTSA
                     TIATGPGPEGFDFFKRETGLDAVQRPTRYLLLPSCFDYRTALQGFVVSDLPEPNEADF
                     EEASVTAVADLIRASNGGALVLFTSVRHREAAKKMLAGFPLPIITQSDSAMGVVMRRF
                     RGEVDSSLLATDTFWEGVDIKGDALRNLIMVRLPFRYPSHPFILRFMKRLEEQTGQDG
                     FSVFTLPHAVLKFKQGIGRLIRTKNDRGVA"
BASE COUNT          931 a         1494 c         1565 g          907 t
ORIGIN      
        1 cgggtggtcg ctgtcgcccg tcccgttgtc gccggtcgcc agttcgtcat tcggggcgtt
       61 cgtgttctcc tgcgagcagg aaagggtgag cgccatgagc gccgtcacgg cgatcgcaag
      121 gaacttcgtc atgcggggtc ctcctcccag tgggccgtta ggtccggata cgacaacgag
      181 tatacggaca atgagagcgg aaggcaagga gaaagatgca gtataaataa ttgacaagaa
      241 tggccttatt ctttatatag gggttcgttc gtcgtgatat cgatcgcgtt cgttgagcat
      301 ggaggtgttg catgtcttat ctcaaatcgc gtggcttcag accggccaag aatgagcccg
      361 aggaaaagaa tatcgccgaa aaggacgcct ttcaggagca ggggcagcgg ctgatagacc
      421 ggcttgcctc gcacccctat ttcatcatcg gcacggtcgc ggcggtcatc gtcacggtcg
      481 tgatcgcgat actggtggcc gacaagtggt cggaatcgcg gagcgcgaag gcgcggtcct
      541 tcgtcgacgc gatggcgctc tgggaaggga aagggacgga agggacggcc ccgaaggacg
      601 aaacggcccg gctcaaggag gcgttggcga agttcgccga ggcggaaaag accctgcaag
      661 gctccttcat gggcgacacc gccgccttct atcaggccaa gatacactac cggctcaagg
      721 agttcgactc ggcgataaaa ctgttccagc gcctgcagga ttcgtcgaag ataccgaccg
      781 atatccggtt cggcgcctac gagggggagg cctactgcca cctcgaccgt gacgacccgg
      841 cgagcgccgt cgcggtatgg gagcgttact acgcgctgaa gaacgcgccg ctctaccgcg
      901 atttcgcgct gtattacatc ggcaccgcgt acgagaagat gaacgacgcg gccaaggcgg
      961 tcgagtattt caagcgactc aaggcggagt tccccgaaag cccgctcgtc tccaaggtgg
     1021 ccgaccgtct tccggaagac cggaagccct cgtgacggcc gctcgatgat acgaatcccg
     1081 ctcctcgcca tctttcttct gctcgtcgcc gcatcgctcg gcgccgagga gttggtcatc
     1141 aactgggcgg tcccgctgaa aaacgagttc gcctcgataa agaagcgtga attcgccgcg
     1201 ccgctgtacc gcgacgggct catctatgtc gctctgcgcg acggccggct catggtcttc
     1261 gggcgcgacg gccgtaaggc gggcgagcgc cgcttcaccg gcgggttcgt cgttcctccg
     1321 gtcgagatcg ccggcggggt gctcgtctgc gaatcgcata cggcgcacct gctcgagaac
     1381 ggctccctgc gcgaccgttg ggccgtggcg gggcgttcgc cggtggtcgc ccggcccatc
     1441 tcccagcccg aggggctcta cctgcagttc ctcgaacata cggtctatct ggtcgacccg
     1501 gccgatgggg cggtgaaggc gaactacact ttctacggcg aaagccaact ttcctacctg
     1561 atgctttccc agccggtcgc ctacgaggac aagcgggtgt tcggcttttc caacggtcag
     1621 gtgttctttt tcctgtaccg caaggactcc tacggggtgg acgagatact gcccttctac
     1681 aagtatcaga ccggcgagat acaggtcggc gccgaaaaac gcgattttta cgacatattc
     1741 tcgctcctgc ccggatcgga ggggatgctc ctcttctcga acggcgaggc gggcggatcg
     1801 atacgggtca gcaacggcgc ggtgaccaag acgggcgaac tgcgtaatct cacgctgaag
     1861 gaacgggccg acgggacggt ccttgcgtat ggcgaagcgg gggcgctcgt actcgccaag
     1921 gacggcaccg tggagcgtcg gctgttctca agtccctcat tcgtcaccgg catcgtcgag
     1981 gcggggtcct acgcgctcgt taccgagatg cacggggtcg tttcactgta tcttgccgat
     2041 ctttctcgga aactcgccac ggtcaggatc ccgcagggcg tttcgaacgg cggggtgtgg
     2101 ggggacggtg ccttctacct cctctccgac atggggacgc tctattcgtt cggggtggtg
     2161 aaaaaagaaa aagaccaaaa aatacttgaa aaaagcgcag aaccggatat gaaaggcgac
     2221 agttcacgaa ccaagtaaag acgactggag gagtggatga acaaatcgga ccttatcaat
     2281 gtgctgaccc agcgggcgaa cctttccaag cggaacgccg aacaattcgt caaccttatg
     2341 gtggatgcga tgatcgacgc gatgcgcaag gaagagcgca tagagatccg tggtttcggc
     2401 agtttcgtca tccgtcatta cgatcagtac gagggtcgta acccgcggac cggccagaag
     2461 atacacatcg ccggcaaacg gcttcccttc ttcaaggcgg gcaaggacct cctgcagagg
     2521 gtcaacatct aacgccccat gccttctttt tcgccagagg acttcctgct cccctcggcg
     2581 atagaattgc tgagacatta cataaactcc aatgacggca acgagatact cgcggtcggg
     2641 tcggtgaacg gggacgggtt cgtccacaag gtcgaactgt tcggcatggg gaccgacggg
     2701 gcggtcccgg cggtgctcaa ggcggcgcgg gccggtgatg tgctcatcca caatcacccg
     2761 ggcggcgaca tccgcccctc cgatgccgac atcggcatcg cctccgaggc ggccgaaaaa
     2821 ggtgtcggct cctacatcgt cgacaacggg gtcacccgca tctttcccat cgtgcggtgg
     2881 atgccggtcc gcgacgagga gttccacccg gtcgcgccgg aggaggtcgc cgcggtattc
     2941 gccgagaagg ggcggatagc cgcatcctac cccgaattcg agttccgccc gccgcagtcc
     3001 gaaatggcgc tcgcggcgac cgaggcggtc aacggcggga gcatgctcgt cgtcgaggcg
     3061 gggaccggca ccggcaagag tttctcctat ctcgtgccgc tcctgctcta tgtggcgatg
     3121 aacgaggggc gcaaggcggt cgtgagcacc tcgaccatcg cgctcgagga gcagttgtcg
     3181 ggcaaggaca tcccgttcct gcaggagcat ctcgggctct cgcatatccc ggtcgcggtc
     3241 ctcaagggac ggcagaacta tctgtgcctc cgcaaatacg acctctaccg gcgcgggacg
     3301 ctcgaactgc ccttcgacgg caggaacggt cgcagcgcat cgctggatga cctcgacgcg
     3361 tggatcggcc tgccgcacga cgggtcgaag agcgccatga acaccgcgat agagagcgag
     3421 gtgtggggcg agatctgctg tgacgagcat tcgtgcgacc gcgccaagtg ccggttcttc
     3481 tccaaatgct tcttcttccg gtcgcgccgg caggcgaact tcgcgcagat actgctcgtc
     3541 aaccaccacc tcctgatggc cgacatcgcg ctccgcgacg aggaggagca tgctgcgggc
     3601 atcataccgc cctacgacat actggtgata gacgaggcgc acaacctctt caaggcggcg
     3661 gtcagtttcc tcggcgagtc ggcgagtctg tcgggtatac tgcggtcgct gaaacggctc
     3721 ttcagcaacg atcggggcac cgggctcctc gcgcgactca tcgacgccta tgccgagccc
     3781 gatacggtcc gcgacctcga acggctggcc ggcgagatcg ccgcgttcgt cccgttctac
     3841 atccactcga tcgtccccga atgcatggag cggttcaagg gggccaagga gaactatctt
     3901 tcgctcgacg atcttgcgat gcgcggcgag atgcgtgaac agttcctccg cgtgacgcag
     3961 ttcctcggcg cgttctgcga acggctcggg ccggtgctcg accgcatcgg gacgagactt
     4021 tccgacgatc ctctcagccg gtcgcgcgag gaggacatga tagcgtcgct tctcaccgag
     4081 acgcgtggca ccctgcgccg tctcgagaac gcgaaggagt tcctcgacct gttcttcacg
     4141 agcgccgaca ccgacggcgc cgtcttctgg ggagaaaagg tctcggcgac caacctgcgg
     4201 ctgtcggtca cgccgctcga catccagcgg atattcgtca ggagcatcta cgagaaggtg
     4261 cggagcgtcc tcttcacctc ggcgaccatc gccaccgggc cgggaccgga ggggttcgat
     4321 ttttttaagc gcgaaacggg tctcgacgcg gtccaacgcc cgacgcgcta cctgcttctc
     4381 cccagttgct tcgattaccg gaccgccctg cagggcttcg tggtgtcgga cctgccggag
     4441 ccgaacgagg ccgatttcga ggaggcgagc gtcacggcgg tggccgacct catccgcgcc
     4501 tcgaacggcg gcgcgctcgt gctgttcacc tcggtcaggc accgcgaggc ggcaaagaag
     4561 atgctcgcgg ggttcccgct tccgataatc acgcagagcg attcggcgat gggggtggtg
     4621 atgcggcggt tccgcggcga ggtcgattcg tcgctccttg ccaccgatac cttttgggag
     4681 ggggtggaca tcaaagggga cgccctgcgc aatctcatca tggtgcgcct gccgttccgg
     4741 tatccgtccc acccgttcat cctgcggttc atgaagcggc tcgaggagca gaccgggcag
     4801 gacgggttct ccgtctttac cctgccgcac gcggtcctca agttcaagca ggggatcggg
     4861 cggctcatcc gcacgaagaa cgaccggggc gtggcgg
//
LOCUS       DAOQUE010000189         4888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_19194_length_4888_cov_4.953652, whole genome shotgun sequence.
ACCESSION   DAOQUE010000189 DAOQUE010000000
VERSION     DAOQUE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19194_length_4888_cov_4.953652"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKH10_06845"
     CDS             complement(<1..187)
                     /locus_tag="PKH10_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNT27877.1"
                     /translation="MEARGIPRGRLRVLLKRGEVERLARGLYRLTSVEPSEMETVAMV
                     AAAVPGGIVCLLSALQMQ"
     gene            complement(792..1709)
                     /locus_tag="PKH10_06850"
     CDS             complement(792..1709)
                     /locus_tag="PKH10_06850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT27878.1"
                     /translation="MRDSRRAITLIILTFNEEKHIERCIKNAQAVASEVFLVDCCSTD
                     RTRAIAEGLGAKVVDHAWPGNHAAQLNWALENLPITTPWVMRLDADEYLTDELAAEIN
                     AVLPTMSSEMSGVYFKRRVYFMGRWIKHGGYYPTFLLRLWRHKKVVCEQRWMDEHMKV
                     VEGTSVTLDHDFVDDNLNYLPWWTAKHNGYSSREAVELLNHEHHFMQADVLGNGGDGS
                     QEKTKRKLKENYYSRLPLFLRALIYWKYRYFLKLGFLDGVPGLIWHFLQGFWYRFLVD
                     AKIYQVERYAKDRGVSMKEAIKEVLGIEL"
     gene            complement(1699..2610)
                     /locus_tag="PKH10_06855"
     CDS             complement(1699..2610)
                     /locus_tag="PKH10_06855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT27879.1"
                     /translation="MENKKPGICPVCLSKAYFWKEKTSKKIRYRIEKCDNCGHAFVNP
                     RPSFDEIAAFYSNSEQNKKEYTVTEETIINERKKQHEETGDAHDLIKEIENTNTNKEC
                     KAFLDVGCGSGFFSKEATEKGFDVVSLELSSGKRAIAKKLLGKEPLATSFEDYDSEQR
                     FSVILMSQILEHVVDINLWIRKANWLLAREGVLVISLPNFNSVFRFFLQEKDPFICPP
                     IHLNFFNSKSIKLLLEKHGFRIKKIENISRIPSSVLEKRIPGLFRTTVPAWTLFANIL
                     LKIMNLFHMGMMINVFAVKKENVDNER"
     gene            complement(2649..3419)
                     /locus_tag="PKH10_06860"
     CDS             complement(2649..3419)
                     /locus_tag="PKH10_06860"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNT27880.1"
                     /translation="MKYVQSSTVGVFPQVKELLASGRKVLFSGTPCQVAGLRNFIGDD
                     AEGLTTCDLVCHGVPSPGVFARYMEEQEKKYEAKTRGYSFRHKKTGWNFMEVDQVFSE
                     GRSYHSMDWADPFMHGFLKNILLRPVCYRCPFYPVPRIADLTLADYWSVATKYPKYDD
                     DKGTSLVLIHSKKGDGLLHAGNALIVHRTDIAHGIAHNAHLNHPAAEPACRKEFFEAF
                     RGGSFSVAASVYIKKGEVLRRKIRRWIKKTIWRLRFNK"
     gene            complement(3416..3820)
                     /locus_tag="PKH10_06865"
     CDS             complement(3416..3820)
                     /locus_tag="PKH10_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT27881.1"
                     /translation="MYLFTGEKKDCCGCMACVPVCPVNCITVAVDEEGFEYPVRDAAR
                     CTNCGKCTAVCPVVNKPEAIPTEGFPAAYAAWSRDAETRRNSSSGGVFPELAKRVLSD
                     GGVVFGAAFSDDFHTVHHIKMEDPGDLPRLQG"
     gene            complement(3821..>4888)
                     /locus_tag="PKH10_06870"
     CDS             complement(3821..>4888)
                     /locus_tag="PKH10_06870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNT27882.1"
                     /translation="FHYANNYGAVFQSYALYAYLRSIGHEVEVIDYQSDDIINGGSFR
                     CPFSRTAIKADIYTLYIKWRKFRKISTEDHAKTAFEEFRNKFLTISNRRYTSFAGLKS
                     DPPLYDAYVCGSDQIWNPPIHVGVDPAYYLDFAPTGRKRIAYAASFGRNDIEPEYRAE
                     MARLLGKMDALSVREKNGADLVEMLTGREARWMPDPTLLLDDYTAITAPVPESGHLFT
                     YILREKEWVEKANRLVADKLQASIVDPHEKKMSPTEWVGCLKQAKFVVTNSFHGTVFS
                     IIFRKPFVTVGLTGKKAPLNDRMLSLLGRIGLEGRFLAAYDEAALARLVDAPIDWDAA
                     HSRLKEWRAEAHRYLTEALGG"
BASE COUNT         1091 a         1420 c         1133 g         1244 t
ORIGIN      
        1 cctgcatctg aagcgccgaa agcagacaga cgatccctcc cggcaccgcg gcggccacca
       61 tcgcgaccgt ctccatctcg cttggttcca ccgatgtcaa ccggtacaga ccgcgcgcca
      121 atcgttcgac ctctcccctc ttgagcagaa cacgcaggcg tccccgggga atgccgcgcg
      181 cctccatatc cgaaggacgg aacaacgcag gcatgttttt tggaaaagtc gttccgttac
      241 ccaccggtgc ccccttctcg atcactcaat atgtcgacag ttgcgtctat atgccgacat
      301 tctgttcacg gaatcccgtt aaacaaaacg atatcgttat cttatatttt tctttctcta
      361 tgtcaaactt attgtgtttc cccgccgagg atccgactaa aaccctcatc cctgccttct
      421 cccaatgacc tcaccggtct tcgcctttgg cttcgcaccg gcaagcccct tcccgtcccc
      481 ttatctctta cctcatcctg tctccttctc ccaatgacct caccccgtcc cctctccttg
      541 aaaaatgaga ggggctgctg tttccgccga ggaccgcgga tgcgcgaatg cgcggagctc
      601 cgcaaggcgt ccgcttctta attaaaaaaa cgcttcttgc ttccttcttg tcgttacaca
      661 agaaggaaga cgccgacagc gtgcgcagca ggcgaggcgg aaagatcgca ttgcgctcgc
      721 cgaaactgct attaattaaa cccttccctc ttactgcctt cccttgacaa ggaaggcgaa
      781 ggtgtgttcc cctacagttc aatccccaac acctccttta tcgcctcctt catggatacc
      841 ccgcgatcct tcgcgtacct ctccacttga tatatcttcg catcgacgag gaaccggtac
      901 cagaacccct gcaggaaatg ccatatcagc cccggcactc catcaaggaa tccgagtttt
      961 aagaaatagc ggtatttcca atatatcaaa gcgcgcagaa agagtggcag acgcgaataa
     1021 taattctctt tgagtttccg tttggtcttc tcctgtgacc cgtcgcctcc gttgcccaac
     1081 acatcggctt gcatgaaatg atgttcgtgg ttgagcagtt ccaccgcctc acgcgacgaa
     1141 tagccgttgt gcttcgccgt ccaccacggc agatagttca ggttatcatc aacgaagtcg
     1201 tggtcgagcg tgaccgatgt cccttccact accttcatgt gctcgtccat ccaccgctgt
     1261 tcgcagacca ctttcttatg ccgccacagc cgcagaagga aggtcggata gtagccgccg
     1321 tgctttatcc accggcccat gaaatagacg cgccgcttga aatagacgcc gctcatctcg
     1381 ctggacatcg tcggcagtac ggcgtttatc tcggccgcca gttcatcggt cagatactcg
     1441 tcggcatcga gccgcatgac ccacggcgtc gttatcggca gattctccag cgcccagttg
     1501 agttgcgccg catgattccc cggccacgcg tgatcgacga ccttcgcgcc gagtccttcg
     1561 gctatcgccc gcgtccggtc ggtcgaacag caatcgacga ggaacacctc cgacgccacc
     1621 gcctgcgcat tcttgatgca ccgctcgata tgtttctctt cgttgaaggt caggatgatg
     1681 agagtgattg ctcgtcgact atctctcatt gtcaacattc tcctttttga cggcgaagac
     1741 atttatcatc attcccatgt gaaacaaatt cattatcttt agcaagatgt ttgcaaacaa
     1801 ggtccatgca ggcaccgttg ttctgaaaag accaggaatt cttttctcaa gcacagaaga
     1861 gggtattcga gatatgtttt cgattttctt tattctaaag ccgtgtttct ccagcaataa
     1921 ttttatgctc ttagaattaa aaaaattcaa atgtatcgga ggacagataa agggatcttt
     1981 ttcttggagg aaaaatctaa atacgctatt aaaattggga agagaaatga ccaaaacacc
     2041 ttcacgggcc aacagccaat ttgcctttcg aatccagaga tttatatcta caacatgctc
     2101 cagaatctga ctcataagta tcactgaaaa cctttgttca gagtcataat cctcaaacga
     2161 ggtagccaaa ggttctttgc caagaagttt tttagcgata gctctttttc ctgaagacag
     2221 ttctagagat acaacatcaa aacctttttc tgtagcctct tttgaaaaga atccagaccc
     2281 acatcccaca tccaaaaagg ccttgcattc tttgttggta tttgtatttt ctatttcctt
     2341 tatcaaatca tgcgcatcgc ccgtttcttc gtgttgtttt tttcgctcat ttataatagt
     2401 ttcttcggtc accgtatact cttttttgtt ctgttcagaa tttgagtaaa aagccgcaat
     2461 ttcatcaaaa gaaggtcggg ggttaacaaa tgcatgccca caattgtcac atttttctat
     2521 cctgtatctg atcttcttgc tggtcttttc tttccaaaaa tatgctttag ataaacatac
     2581 tggacaaatg cctggttttt tgttttccat aaatctttct ctcttaggat tctatggtca
     2641 tgggtttttc atttattaaa acgcagtcgc caaatagttt tctttatcca tcgtcttatt
     2701 tttcttctca ggacctctcc ttttttaata tatacggaag ccgccacgct gaaggaaccg
     2761 cccctgaacg cctcaaagaa ctcctttcgg caggccggtt cggcggcggg atgattcaga
     2821 tgggcattgt gggcgatgcc gtgggcgatg tcggtccggt gaacgatgag cgcgttcccc
     2881 gcatgaagca ggccgtcgcc tttcttcgag tgtatcaata ccagcgaggt ccctttgtcg
     2941 tcatcatact tggggtattt cgtcgcgacg ctccagtagt cggcaagcgt caggtcggcg
     3001 atgcgcggca cggggtaaaa aggacaccgg taacagaccg ggcgcagaag aatgtttttt
     3061 agaaaaccgt gcatgaacgg atcagcccaa tccatcgagt ggtatgatct tccttccgag
     3121 aaaacctgat cgacctccat gaaattccaa ccggttttct tatgtcggaa ggaatatcct
     3181 ctcgtttttg cttcgtattt cttctcctgt tcctccatgt atcgtgcgaa cactccgggc
     3241 gacggaacgc cgtgacacac cagatcgcag gtggttagtc cttcggcatc atcgccgatg
     3301 aagttccgca gaccggccac ttgacaggga gtcccggaaa agagcacctt ccgccccgag
     3361 gctagcaact cctttacctg cgggaacacc ccgaccgtcg aactctgcac atacttcacc
     3421 cttgtagccg tggcagatcg ccgggatctt ccattttgat atgatgaacc gtgtgaaagt
     3481 catcgctgaa cgccgcgcca aagaccactc cgccgtcgct caagactctc ttcgccagtt
     3541 cggggaacac cccgcccgac gaactgttcc tcctcgtttc ggcgtctcta ctccacgcgg
     3601 cataggcggc ggggaaccct tcggtaggta tcgcctccgg tttattcacc accggacaga
     3661 cagcggtaca tttcccgcag ttcgtacagc gggccgcatc gcgcaccgga tattcgaacc
     3721 cctcttcgtc caccgctacc gtgatgcagt tcacgggaca gaccggcaca caggccatgc
     3781 atccgcaaca atctttcttt tcgccggtga acagatacat ctaacccccc aacgcctccg
     3841 tcagatagcg gtgtgcctcg gcgcgccatt ctttcaagcg ggaatgagcg gcatcccaat
     3901 cgataggcgc atccaccaac cgggcaagcg ccgcctcatc gtatgccgca aggaatcgcc
     3961 cctccagccc gatccgtccc aacagcgaca gcattctatc gttcagcggc gccttcttgc
     4021 cggtcagacc gaccgtcacg aagggcttgc ggaagatgat gctgaagacc gtaccgtgga
     4081 acgaattggt caccacgaat ttcgcctgtt tgaggcagcc gacccattcg gtgggcgaca
     4141 tctttttctc atgcgggtcc actatcgacg cctgcaactt atcggcgacc agtcggttcg
     4201 ccttttcgac ccattccttc tcgcgcagga tgtaggtgaa gaggtgtccg ctctccggga
     4261 ccggcgcggt aatggcggtg taatcatcca acagcagggt cgggtcgggc atccaccgcg
     4321 cctctctgcc ggtaagcatt tcgacaaggt cggcaccgtt cttttcgcgt accgacaggg
     4381 catccatctt tcccagcagt cgcgccattt cggcgcggta ctccggctcg atatcgttcc
     4441 ggccgaaact ggcggcgtat gctatccgct tgcgtcccgt cggagcaaag tccagatagt
     4501 aggcaggatc aacaccgaca tgaatgggcg ggttccatat ctggtcactg ccacagacat
     4561 aggcgtcata aagcggcgga tcgctcttca acccggcgaa cgaggtatac cgtctgttcg
     4621 atatcgtcag aaatttattg cggaattctt cgaacgccgt ctttgcgtgg tcttcggtcg
     4681 atatcttgcg gaacttccgc catttgatgt agagggtgta gatgtcggcc ttgatggcag
     4741 tacgggaaaa cggacaacgg aacgatccgc cgttgatgat gtcgtccgat tggtaatcga
     4801 tgacctcgac ctcatgcccg atcgatctca ggtaggcata cagcgcatag gattgaaaga
     4861 ccgcgccgta gttgttggcg tagtggaa
//
LOCUS       DAOQUE010000190         4886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_19201_length_4886_cov_5.470710, whole genome shotgun sequence.
ACCESSION   DAOQUE010000190 DAOQUE010000000
VERSION     DAOQUE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19201_length_4886_cov_5.470710"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="PKH10_06875"
     CDS             <1..1290
                     /locus_tag="PKH10_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27883.1"
                     /translation="YYREDGSIHFCRGCDTPTAKDPQCAQNLWKEANLKLTTDHPEAD
                     CYPYPCEMGNLKPMTKEEVETQYTTIAMHECDLMLANYGWDTDSTRGQIKHWNLSEGK
                     VGFVMYKVDIDITQYITKRKFFLYDTASQKYTAISPGDYGFTYQKNNAIVYLRDSRSL
                     DHATTHMYVGYFSTDGTYRVVFDKPIYSILYSPALNEKWAFANINFAKNGVSTMYYAK
                     VGEWKWTALGTGVAYYPDLHGDLLAFYDNNIKGYVCDLNKSPKSLSDCIVVNRDTEQV
                     RNMQFDRDHPGIFYYSRDFNQAITRVDMTEGAGKWVYEDVITDFSDATKGVAYSLVVQ
                     QVKNNTILYLEIEDFNGTEGGGLACFYRIDTKKRYCMKKMENDPQYGEWVDFPYGHSE
                     FEDHWYLYQKHVNSPLILRDMKCCCEKEGICPFEGMK"
     gene            1287..2435
                     /locus_tag="PKH10_06880"
     CDS             1287..2435
                     /locus_tag="PKH10_06880"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HNT27884.1"
                     /translation="MRAARVATGIYIHVPFCVSRCAYCDFFSTVPEDDSLKAAYGDRV
                     VTHLSLELARMRAGDLVSLYLGGGSPSTMPPSFFTALGAALRNAGIEPAALEFTVEAN
                     PADLSARSLDLFAATGVNRLSLGVQAADDAVLAAMGRRATRATLKKILPPAREKFGDL
                     SYDIIYGFGKRPRDLAAELAWLFDLAAPDHLSAYCYTRPHRRAAPPIAPEDTAIEEEE
                     AIARFLTTRRFRRYEVSNWAQRGLESVHNRLYWSWDRYIGIGAGAHGFEPREGIRYRY
                     PDSVAAFIRRPAPVVERLPRETLMREFTMLALRTAEGVPLAKFSRTFGISLPKLLSPV
                     TRARFLDTGFARLSATRLRATNRGLSFLNTLTAAIFDDIERHERDHAG"
     gene            2425..3036
                     /locus_tag="PKH10_06885"
     CDS             2425..3036
                     /locus_tag="PKH10_06885"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNT27885.1"
                     /translation="MRDDIDTRYRAAGLAVIGVDEAGRGPLCGPVVVAAVHLLAPVAG
                     LDDSKKLSEKQRESLVPEILRNSIWQVYSVLPEVIDNKNILHATLWGMGRAAAKVHEK
                     LAGDKIVLIDGNRLIKRFPREEAIVKGDGKSASIAAASILAKVHRDRIMTRWALIYPQ
                     YGLAEHKGYPTDSHYAALARFGPTPIHRRSFRLVKQPEQGELF"
     gene            complement(3033..3419)
                     /gene="trxA"
                     /locus_tag="PKH10_06890"
     CDS             complement(3033..3419)
                     /gene="trxA"
                     /locus_tag="PKH10_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNT27886.1"
                     /translation="MVATRRRHMENLTKETFKQKVFDYEKNKEWKFAGERPAIVDFYA
                     DWCGPCKAVAPVLADLAAQYDGKVDIYKVNTDQEQELAQVFGIRSIPSILFIPMEDKP
                     QMAMGALPKSAFVDTIENLLLKKPAA"
     gene            3559..4416
                     /locus_tag="PKH10_06895"
     CDS             3559..4416
                     /locus_tag="PKH10_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27887.1"
                     /translation="MPINIPPFWAKVEHREEKGSVIVNGWSDRSATEALENAQKRAKK
                     VFDLVMSGKKLGEYDYSTLPIREQILETVTHDGRQVAVISRNRYGAAVLNTEKVLFAD
                     VDFKPFSAGGFFKALALLFNPGKKKELAETQRREEIARVSSWGEKNRDHPFRLYRTFA
                     GMRLLFTGRTYDPKDTETIRVLESLGSDPLYVKLTKNQSCFRARLTAKPWRIGLDRPP
                     AAFPFLNNREGGKYRHWLDDYLEKSARYRCCDIVNEGWHRPTDPEIRKIVELHDRLTG
                     VSAKDLPLA"
     gene            4547..>4886
                     /locus_tag="PKH10_06900"
     CDS             4547..>4886
                     /locus_tag="PKH10_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27888.1"
                     /translation="MGRWLFLLLFLLIGCSAPEEEDIDTDEGISDGALDTDGLPDFDN
                     ADAPAFCNENDKIDCFGTKLYQCVKGVWTLLTDCAVNNMKCVKPPSGPASCVVKMCGD
                     GGVDTDIGEVC"
BASE COUNT         1056 a         1466 c         1434 g          930 t
ORIGIN      
        1 tactaccggg aggacggctc catccatttc tgccgcggat gcgacacgcc gaccgcaaag
       61 gatccgcaat gcgcacagaa tctttggaaa gaggcgaatc tcaaactgac caccgatcat
      121 cccgaggcag attgctaccc gtatccgtgc gagatgggta atctgaaacc gatgacgaaa
      181 gaggaagttg aaacccaata cactacgatt gccatgcatg aatgcgacct tatgcttgcc
      241 aactacggct gggacaccga ctccacccgc ggtcagatca agcattggaa tttgagcgaa
      301 gggaaggtgg ggttcgtgat gtataaggtg gacatagata taacgcagta tatcacaaaa
      361 aggaaattct tcctctatga caccgcatct caaaaatata ccgcgataag tcccggagat
      421 tatggcttta cttatcagaa aaacaacgcc atagtttatt tgcgggatag tcgaagcctc
      481 gatcatgcga caacccatat gtatgttggt tattttagca cggacggcac ctaccgggtg
      541 gttttcgaca agccgatcta ttccatactc tactctcctg cgctcaacga gaagtgggcc
      601 ttcgcgaaca tcaattttgc gaagaatggt gtctcgacca tgtactacgc caaggtaggc
      661 gaatggaagt ggacggcgct gggaacggga gttgcctact atcccgatct tcacggcgat
      721 ctgcttgctt tctacgacaa caatatcaaa ggatatgttt gtgatctgaa caagagtccg
      781 aagtcactga gcgattgtat cgtggtgaat cgagacacgg aacaggtgcg caacatgcaa
      841 ttcgaccgtg accatccggg tattttctat tattcccgcg acttcaatca agcgatcact
      901 cgtgttgaca tgacggaagg agcggggaag tgggtttacg aagatgtcat caccgatttt
      961 tccgatgcga ccaaaggcgt ggcctattca ctggtcgtgc agcaagtgaa gaataacact
     1021 atcctgtacc ttgagataga agatttcaat ggaacggaag ggggaggcct tgcctgtttc
     1081 taccgcatcg acacgaagaa acgctactgc atgaaaaaga tggagaatga cccccaatac
     1141 ggggaatggg ttgacttccc ctacggtcac tcggaatttg aagaccactg gtatctctac
     1201 cagaaacacg tgaactcccc cctcatcctg cgcgacatga agtgttgctg tgagaaggaa
     1261 ggcatctgcc cgttcgaggg gatgaaatga gggcagcgcg agtcgccacc ggcatataca
     1321 tacatgttcc cttttgcgtg tcgcggtgcg cctactgcga tttcttctcg acggtgccgg
     1381 aagatgactc gctcaaagcg gcctacggcg accgcgtcgt aacgcacctg tcgctcgaac
     1441 tggcgaggat gcgcgccggc gatctcgtgt cgctctacct cggcggcggt tccccctcga
     1501 cgatgccgcc ctccttcttc accgcgctcg gcgccgccct gcggaacgcc gggatagagc
     1561 ctgcggcgct ggaattcacc gtcgaggcga atccggccga tctctcggcg cggtcgctcg
     1621 acctgttcgc cgccaccggc gtcaaccggc tctcgctcgg cgtacaggcg gccgacgacg
     1681 cggtgctcgc cgcgatgggg cgccgcgcga cgcgggcgac gctgaaaaag atactgccgc
     1741 ccgcccgtga aaagttcggc gatctctcgt acgatatcat ctacggcttc gggaagcggc
     1801 cgcgcgacct cgcggcggaa ctcgcgtggc tgttcgacct cgccgcgccc gaccatcttt
     1861 cggcctactg ctatacccgg ccgcaccgcc gcgccgcgcc gcctatcgcg ccggaggata
     1921 ccgccatcga agaggaggag gccatcgccc gcttcctgac cacccgccgg ttccgtcgct
     1981 acgaggtgag caactgggcg caacgcggtc ttgaaagcgt ccacaaccgg ctctactggt
     2041 cgtgggaccg ctacatcggc atcggcgccg gggcgcatgg cttcgaaccg cgcgaaggga
     2101 tccgttaccg gtatcccgac tcggtcgccg ccttcatccg gcgtcccgca ccggtcgtcg
     2161 aacggcttcc ccgggagacg cttatgcgcg agttcacgat gctcgccctg cggaccgccg
     2221 agggggtgcc actcgccaag ttctcaagaa cattcggcat atcgttgccc aaacttcttt
     2281 cgccggtgac gagggcgcgg tttctcgaca ccgggttcgc ccgtctgtcg gcgacccgtc
     2341 tgcgggcgac caaccgcgga ctttcctttc tcaacacgct caccgccgcc atcttcgacg
     2401 acatcgaacg ccacgagagg gaccatgcgg gatgatatcg acacccgcta ccgcgccgcg
     2461 ggactcgccg tcatcggcgt ggacgaggcg gggcgcggcc cgctgtgcgg cccggtcgtc
     2521 gtcgcggcgg tccatctcct cgcgccggtc gcggggctcg acgattcgaa gaaattatcg
     2581 gaaaaacagc gcgagtcgct cgtgcccgag atactgcgca actcgatctg gcaggtctat
     2641 tcggtcctgc cggaggtgat agacaacaag aatatcctcc acgcgacgct ctgggggatg
     2701 gggcgcgccg ccgcgaaggt ccatgaaaaa ctcgcggggg acaagatcgt cctcatcgac
     2761 ggcaaccggc tcatcaagag attcccgcgc gaagaggcga tcgtgaaggg ggacggcaag
     2821 agcgcgagca tcgcggccgc ctcgatactc gccaaggtgc accgcgaccg tatcatgacc
     2881 cgctgggcgc tcatataccc gcagtacggg ctcgccgagc acaaaggcta tccgaccgac
     2941 agccactacg ccgcgctcgc ccggttcggg ccgacgccga tacaccggcg cagtttccgg
     3001 ctcgtgaaac agccggaaca gggggagttg ttctaggccg ccggcttttt caacagcaag
     3061 ttctctatcg tatccacaaa ggccgacttg gggagcgccc ccatcgccat ctgcggcttg
     3121 tcctccatcg ggatgaagag gatggagggg atggagcgga tgccgaagac ctgcgccagt
     3181 tcctgctcct gatcggtgtt caccttgtag atgtccacct tgccgtcata ctgggcggcg
     3241 aggtcggcga gcaccggggc gaccgccttg cagggaccgc accagtcggc gtaaaagtcg
     3301 acgatggcgg gacgctcgcc cgcgaacttc cattccttgt tcttctcgta gtcgaacacc
     3361 ttctgcttga aggtctcttt cgtcagattt tccatgtggc gcctccgtgt tgctaccagc
     3421 aagcaagttt atagggacgg ttctcgggaa gtcaaggaga cggtaaagaa ttttcaaaaa
     3481 gaaatatctt gcttcgaggc gacacaaaag taaaatcagg aggttcctgg gaaaaaggtt
     3541 gaacggaggg aggtcgtcat gccgataaac atcccgccct tctgggcgaa ggtagagcac
     3601 cgcgaggaaa agggttcggt catcgtgaac ggctggtcgg accgttcggc gaccgaggcg
     3661 ctcgagaacg cccagaagcg ggccaagaag gtgttcgacc tcgtcatgtc gggcaagaaa
     3721 ctgggcgagt acgactactc gacgctcccc atccgcgagc agatactcga aacggtcacc
     3781 cacgacggca ggcaggtcgc cgtcatatcg cgcaaccgtt acggcgcggc ggtactcaac
     3841 accgaaaagg tcctgttcgc cgatgtcgat ttcaagccgt tctccgccgg cggattcttc
     3901 aaggcgctcg cgctcctgtt caacccgggg aagaaaaagg aactcgccga gacccagcgg
     3961 cgggaggaga tcgcccgggt ctcatcgtgg ggcgagaaga accgcgacca tcccttccgg
     4021 ctctaccgca ccttcgccgg gatgcggctc ctgttcaccg gccgcaccta cgacccgaag
     4081 gacaccgaaa cgatacgggt gctcgagtcg ctcggctccg acccgctgta tgtgaaactc
     4141 accaagaacc agtcgtgttt ccgcgcacgg ctgaccgcca agccgtggcg catcggactc
     4201 gaccgcccgc ccgccgcctt cccgttcctc aacaaccgcg agggaggtaa gtaccggcac
     4261 tggctcgacg actatctgga gaaaagcgcc cgctaccgct gttgcgacat cgtgaacgag
     4321 gggtggcacc ggcccaccga tcccgagata cggaagatcg tcgaactgca tgaccggctg
     4381 accggcgtat cggcgaagga tttgccgctc gcgtaagtcg cctcgaccct tggatactca
     4441 caaagaatat cttgattctg gattcgggtg tgagtagaat cgatcggtca tcaaaggaag
     4501 agccgcatca atgcaaacca tagagatcac cagaacggag gccgccatgg gtcggtggtt
     4561 gtttcttctg ctttttcttc ttatcggctg tagcgcaccg gaggaagagg acattgacac
     4621 ggacgaaggg ataagtgacg gtgcgctcga caccgatggc ctgcccgact tcgataacgc
     4681 cgacgcgccc gccttctgta acgagaacga caagatagac tgtttcggca cgaaacttta
     4741 ccagtgcgtc aagggggtct ggacccttct taccgactgc gccgtcaata atatgaaatg
     4801 cgtgaagccg ccgagcggcc ccgcctcctg tgtcgtcaag atgtgcggcg acggcggggt
     4861 cgacaccgac atcggagagg tctgcg
//
LOCUS       DAOQUE010000191         4859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_19330_length_4859_cov_4.579933, whole genome shotgun sequence.
ACCESSION   DAOQUE010000191 DAOQUE010000000
VERSION     DAOQUE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19330_length_4859_cov_4.579933"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PKH10_06905"
     CDS             <1..901
                     /locus_tag="PKH10_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27889.1"
                     /translation="YRLIAARLQIPPSCQLKEKEDKIVEIDKKIQNALDEFDKADKSK
                     QGENESVKMKNEDEDRKFLVEVEKEYQKKFDELNQRLSEFDSKESLKKEDLTKFDKEI
                     EEYMSKNAEEMGKIQEALEKERTDLEKAYNEKKAAAQTDFETKYKTKLDELNAKKTEI
                     DAKIASWDADTDKMKQEKEAALTAENKAGDAKKELAKIDKDRKAEKAKLEKEQKKVTG
                     DLAAHEKGKEEAQMNAIMAIDMEFEAKFADVDTRQVKDNDRLNKLNNDSVKRNSRRRK
                     DTKRVSSDSLRTRTPTARTSKNS"
     gene            1036..1938
                     /locus_tag="PKH10_06910"
     CDS             1036..1938
                     /locus_tag="PKH10_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27890.1"
                     /translation="MKERLAKEIEDITMKIEQVNAKWAKDQEDLRVKQQEAADKFTAN
                     WKERSEALEKSYAEKKDAVVKKYEKIETDEKNANKDMKTKVEQDIDALLKEKNRRKES
                     RQTLLNTEKSTWEDQKKKWAADKEQRKVDLDKFKVTSDEEIKAERTRIIEKKRDIEKK
                     YEETIKAINDEETSQIQAQFKDVSKEVSKLEVTPSKMTIDIEAMKADTLARQGLLYLQ
                     KEDLIASRRAFVNALYLDRESRTAIDGLKAIETTAKSMYWKAYGLRDTNKKDAQKILE
                     TLIKTLMPSSEYFIKAKTALEEIK"
     gene            1966..2151
                     /locus_tag="PKH10_06915"
     CDS             1966..2151
                     /locus_tag="PKH10_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27891.1"
                     /translation="MVCCKKAPKKTAKKVVKKAVKKVVKKVAKKPVKKVVKKVAKKPV
                     KKVAKKVAKKTVKKAKK"
     gene            complement(2220..3620)
                     /locus_tag="PKH10_06920"
     CDS             complement(2220..3620)
                     /locus_tag="PKH10_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27892.1"
                     /translation="MRTSLPAILAFILLGGYQTLPAQEEAPAKVAPPAPLEERLQAIE
                     QQMRDTEAEQQRLLEENRGLDAKRPEPSSPEKKEKEPIAFAPYGFIEMIGWANDSLFV
                     GNDLPLYVSDRGQSTTGVTAKGSRLGTKILFPRLEAVTLTATLEIDFVANMADTGYAE
                     SYPGIRLRHALMDLSKTWGDSTLGFKAGQTWATALPNVWPALINPACGWGIGNLWQRM
                     PLAEIYFTQRFAETFAFTAQVAAARAMTGASANRNSFLEVNIDAGDASHIPQLHGQLS
                     LSGRFSDIDLLFAVGGAWGRENYKGGVYLNKKDIRHIGGMTDVGLVVSSLKLSHQYAE
                     VAGKFFWGQNLDTFGVFGGSLITEDIDATTRRVTGSQRALGWWAQLTMKPLKELALHV
                     GYSSEDPDEKQSGATPLYFNNDALWVSAFYTFFERLTLGFQWIQMQTHDIKLTGVTER
                     RSMTGNSIMGNVKLTF"
     gene            3751..4383
                     /locus_tag="PKH10_06925"
     CDS             3751..4383
                     /locus_tag="PKH10_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT27893.1"
                     /translation="MQVFRLKAAEGHHETMVAVHDDRQLIRAAKNGDIPSFETLVRRH
                     HRTLYRFALGITHGDEQEAADILQEGLVKAFLNIGRFEERSSFSSWLWRIIRNEFLDR
                     LRRERIETVPLEEGEGPARGAPQDTPESDLVNEERRRALLRLVAALPEKYSEIVILVE
                     LMELSYEDAADYLGIPVGSVRSRLSRARERLTGLVEKNMELFGAGPRHTG"
     assembly_gap    4450..4459
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4460..>4859
                     /locus_tag="PKH10_06930"
     CDS             <4460..>4859
                     /locus_tag="PKH10_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27894.1"
                     /translation="ALPDDFEPMLHRRIVEAAATRPEPVPRWTAFLRPVLVSVAFVAV
                     FAVGIWVGRSAPEKIEESRPVAQQVEPQSLAPQTVIAKTVVKRGEPVTIRLVYDSIKD
                     IDDVRFTIVLDDGVKFRSGDPEIAATGKLE"
BASE COUNT         1276 a         1235 c         1504 g          834 t
ORIGIN      
        1 ctatcgtctt attgctgcgc gtctacaaat tccgccatct tgccagttaa aagagaaaga
       61 ggataagatc gtcgagatag acaagaagat acagaacgcg ctcgatgagt tcgataaggc
      121 cgataagtcc aaacagggag agaacgagtc ggtcaagatg aagaacgagg atgaggatcg
      181 caaattcctc gtcgaggtcg aaaaggagta ccagaagaaa tttgacgagc tcaatcagcg
      241 cctgtcggaa ttcgattcga aggagagtct caaaaaagag gatctcacga agttcgacaa
      301 agagatagaa gaatatatgt cgaagaacgc cgaagagatg ggaaagatac aggaggcgct
      361 ggagaaagag cggaccgatc tggaaaaggc ctataacgag aagaaggctg cggcacagac
      421 cgatttcgaa accaaataca agacgaaact cgacgaactt aacgcgaaga agaccgagat
      481 agacgcgaag atcgcgtcct gggacgccga taccgataag atgaaacagg aaaaagaagc
      541 ggccctcacc gccgagaaca aagcggggga cgcgaagaag gaactggcca agatagacaa
      601 ggatcgcaag gccgagaagg cgaaactgga aaaggaacag aagaaggtga cgggcgatct
      661 ggcggcgcac gaaaaaggta aggaagaagc gcagatgaac gccatcatgg cgatcgacat
      721 ggagttcgag gcgaaattcg cggatgtcga cacccggcag gtcaaggata acgaccggct
      781 caacaaactc aacaatgatt ccgtaaaaag aaactcgagg agaagaaaag atacgaagag
      841 agtttccagc gattccttaa ggacaaggac gcctaccgca agaacatcga aaaactcata
      901 gagtccgccc agaaggagcg cgatgcgaag atcgcgaaac ggaaagagga ccgcgaaaga
      961 cttgcgcagg gatggtccgg tgatcgcgag aaggcgaagc aggcgctcga ccggaagttg
     1021 gaacccgacc gcaagatgaa ggaacgcctc gccaaggaga tcgaagatat cacgatgaag
     1081 atcgaacagg tcaacgcgaa atgggcgaaa gaccaagagg atctgcgcgt aaaacagcag
     1141 gaggcggccg acaagttcac cgccaactgg aaggaacgca gcgaggcgct tgagaagagc
     1201 tatgccgaaa agaaggacgc ggtcgttaag aaatacgaaa agatagagac cgacgagaag
     1261 aacgcgaaca aggacatgaa gacaaaggtg gagcaggaca tagatgcgct cctgaaggaa
     1321 aagaatcgcc gcaaggaaag tcgccagacc ctgctcaata ccgaaaagag tacctgggag
     1381 gatcagaaga agaaatgggc cgccgacaag gaacagcgga aggtcgatct tgacaagttc
     1441 aaggtgacct ctgatgagga gatcaaggcg gaaaggaccc gtatcatcga gaagaagagg
     1501 gatatagaga agaagtacga agagaccatc aaggccatca acgacgaaga gacatcccag
     1561 atacaggcgc agttcaagga tgtgtcgaaa gaggtctcaa aactggaggt gaccccgtcg
     1621 aagatgacta tcgacatcga ggcgatgaag gcggataccc tcgcacgcca aggactcctg
     1681 taccttcaga aggaggatct tatcgcgtcg cgtcgcgcct ttgtcaacgc cctctatctg
     1741 gaccgcgaga gccggacggc catcgacggt ctcaaggcga tagagaccac ggcgaaatcg
     1801 atgtactgga aagcgtacgg gctccgcgac accaacaaga aggacgccca gaagatactg
     1861 gagaccctca taaagaccct gatgccgagt agtgagtatt tcataaaggc gaaaacggct
     1921 ctcgaggaga tcaagtaacc cggtaaccaa gaaatggagg aacccatggt gtgctgcaaa
     1981 aaggctccca agaagacggc gaagaaggtc gttaagaagg cggtgaagaa ggtcgtcaag
     2041 aaagtggcga agaagccggt gaagaaggtc gtcaagaaag tggcgaagaa gccggtgaag
     2101 aaggtcgcca agaaagtggc gaagaaaacc gtcaagaaag ccaagaagta gtctctcgat
     2161 ccccgctaca tcatctttgg gacgcctctt ttgcggggcg ttcttttgtc ttgaaaggtt
     2221 cagaaggtaa gttttacatt acccatgatg gagttgccgg tcatagatct tctttcggtg
     2281 acgccggtga gtttgatgtc gtgtgtctgc atctgtatcc actgaaagcc gagggtgagg
     2341 cgctcgaaga aggtatagaa cgcggagacc cagagcgcat cgttgttaaa ataaagaggc
     2401 gtggccccgg attgcttttc atcaggatcc tccgaactgt agccgacatg cagggcgagt
     2461 tccttcagcg gcttcatggt gagttgcgcc caccagccca gagcgcgttg gctgccggtg
     2521 acgcggcggg tcgtcgcgtc gatgtcctcg gtgatgagtg aaccgccgaa cacgccgaag
     2581 gtgtcgagat tctgtcccca gaagaatttc cctgccacct ccgcgtactg gtgtgacagt
     2641 ttcagcgagg agacgacgag cccgacatcg gtcatgccgc cgatgtgtct gatgtctttc
     2701 ttgttgaggt agaccccgcc tttgtaattc tcgcggcccc acgcgccgcc gacggcgaag
     2761 agcaggtcga tatcggaaaa gcgtccgctc agggagagtt gtccgtgcag ttgcgggatg
     2821 tgcgatgcgt cgccggcgtc gatattaacc tcgaggaacg agttgcggtt agccgacgcg
     2881 ccggtcatgg cgcgcgccgc ggcgacctgt gcggtgaagg cgaaggtctc ggcgaatcgt
     2941 tgggtgaaat atatctcggc gagcggcatt cgttgccaga ggttcccgat accccagccg
     3001 caggcaggat tgatgagagc gggccataca ttgggaagcg ccgtggccca agtttgccct
     3061 gccttgaagc cgagcgtcga gtctccccag gtcttgctga gatccatgag ggcatgacgg
     3121 aggcggatgc cggggtaact ttcggcatag ccggtgtcgg ccatgttggc gacgaaatcg
     3181 atctccagcg tcgcggtcag cgtgaccgct tcaagacgcg ggaacagtat cttggtcccg
     3241 agccgggagc ccttggcggt gacgccggtg gtactctgcc cccggtcgga cacatagagc
     3301 ggcagatcgt tgccgacgaa aagagagtca ttcgcccagc cgatcatctc gatgaagccg
     3361 taaggcgcga acgctatcgg ctctttttcc tttttttccg gcgaggaggg ctcaggcctc
     3421 ttcgcgtcaa ggcccctgtt ctcttcgagg agtcgttgct gttctgcctc ggtatcgcgc
     3481 atctgctgct cgatcgcctg gaggcgctcc tccaacggcg cgggtggcgc gaccttcgcg
     3541 ggcgcctcct cctgcgccgg gagggtctga tagcccccca ataggataaa ggcgaggatg
     3601 gcgggtaacg atgttctcat ctgaagacct ccctgaagac aacggaacgg cccatgctac
     3661 aaaaaagaaa aaggcaagtc aagagcggga aaagaggccg tggcatccgc cgttttgttg
     3721 acaaggggaa gcggcccgat atgcttcggt atgcaggtct tccgcctaaa ggccgctgag
     3781 gggcaccacg aaaccatggt cgctgttcat gacgatcgac aactgatacg ggccgccaag
     3841 aacggcgata ttccttcctt cgagacgctc gtgcgtcgcc atcaccgcac gctctaccgg
     3901 ttcgccctcg gcataacgca tggcgatgaa caggaggcgg ccgatatcct gcaggagggg
     3961 ctcgtcaagg ccttcctcaa tatcggtcgg ttcgaggagc gttcgagttt ctcctcgtgg
     4021 ctttggcgca tcatccgtaa tgaattcctt gaccgcttac ggcgcgaacg gatcgagacg
     4081 gtcccgcttg aagagggaga aggaccggcg agaggcgccc cgcaggatac ccccgaaagc
     4141 gacctcgtga acgaggaacg gcgccgggcg cttctccgtc tggtcgccgc gctccccgaa
     4201 aagtattcgg agatagtgat acttgtcgaa ctcatggaac tttcctacga ggatgcggcg
     4261 gactatctgg gtatcccggt cggatcggtc cggagccgtc tgtcgcgggc gcgggaacga
     4321 ttgaccggac tggtcgagaa aaatatggaa cttttcgggg cgggcccccg tcacacgggt
     4381 tgaaggaggc cggtatgata gacgaacgta acaaacaggc ggaacgactc gtcaccggtc
     4441 tcctttcttn nnnnnnnnna ggcgctacct gacgacttcg agccaatgct ccaccgccgc
     4501 atcgtggaag cggcggcgac ccggccggag ccggtgcccc gctggacggc gttcctccgt
     4561 cccgtccttg tctcggtcgc cttcgtcgcc gtgttcgccg tcggtatctg ggtcggccgg
     4621 tcggccccgg aaaagatcga agaaagcaga ccggtggcac aacaagttga gccccagagc
     4681 cttgccccgc agacggtgat cgccaagacg gtcgtgaagc gcggcgagcc ggtcaccatc
     4741 cggttggtgt atgattctat aaaggatatc gacgatgtcc gcttcaccat cgtccttgac
     4801 gatggcgtca agttccggtc gggcgatccg gagatagccg ccaccggaaa acttgaatg
//
LOCUS       DAOQUE010000192         4843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_19420_length_4843_cov_5.141186, whole genome shotgun sequence.
ACCESSION   DAOQUE010000192 DAOQUE010000000
VERSION     DAOQUE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19420_length_4843_cov_5.141186"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKH10_06935"
     CDS             complement(<1..469)
                     /locus_tag="PKH10_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT27895.1"
                     /translation="MNLFDIIVIGGGHAGIEAAAAATRLGARTLLVTLRIDSIGEMSC
                     NPSIGGQAKGQIVREIDLLGGLMGEAADFSAIQYRVLNRSKGPAVRSTRSQNDRLLYK
                     KFLNDRLLSFPLLTIWQDEATGLLTEKGNITGALLRTAGEVTAGAVVVTAGTFL"
     gene            complement(581..1936)
                     /locus_tag="PKH10_06940"
     CDS             complement(581..1936)
                     /locus_tag="PKH10_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNT27896.1"
                     /translation="MRLAERLGLTIAAPATATGGAIAIIRISGPTTVDVLKKLTGRDR
                     FVPRRATLCSLDLGPRAASEQAIVLYFAAPASYTGEEMGELHLHGGAGTMEEALSRLT
                     ELGATPATPGEFSFRAVVNGKMTLAQAATIPALIATQDRLQADIARRAAYGGLFEQAA
                     VPLLTQWDELDTIANATVDFPEQVTEHLPLAKISVLIEETEHLVIPALENTQRFDRAA
                     TPRILIAGRPNVGKSLLFNRLLGLDRAIVSPEPGTTRDYLTALWPLPFPAVTVELCDT
                     AGLRDIASGIEKDGITRTLTLSEVSEHIILLFDGSDAPTAADIAARALFAPKKPLVFA
                     NKADIARHPAAESMAPDLFISALTGEGLPDMISLLAGRITALLPDTALPILLHAERRR
                     VAERLCAALEELRLLLSAGDLALLVVAISRCRTLLGELVGRSDDPDLYERVFSSFCLG
                     K"
     gene            2048..2779
                     /locus_tag="PKH10_06945"
     CDS             2048..2779
                     /locus_tag="PKH10_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNT27897.1"
                     /translation="MLFDAHIHLSEIPKVEQGRPLPASVLTSAHDPEDPSLSLGDPAV
                     LCSYGLHPLWLDAPGRSLETLVKLSASGQISAVGEFGFDFYHGRDREKEKKQRDFFHA
                     QCVIAERYRLPVVLHARRGMAELFNETTLLKKLSAVIFHCYPGTAEEGQAFLKRGVNG
                     FFSFGTPIIKNYPRAIAALKRLPLDRILFETDAPFQPPHGREMSLPIDIVTVYEKGSE
                     LLSIPRPELEHIVWGTAQGIFPGRL"
     gene            complement(2774..3589)
                     /locus_tag="PKH10_06950"
     CDS             complement(2774..3589)
                     /locus_tag="PKH10_06950"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNT27898.1"
                     /translation="MPNDGSTSSLSTERSFVPLLDLLLFYRSRRILLIWSLFIFLIFF
                     MIMLLVLSFFTNFVSSQIQFVIPAPAEEDSPLWHLLFWFLNGMFLVCLRAASFFLSFL
                     PAYVLASPFLQFLSISAEALYHGKKLKDDAFPDIDEVLGDLKDGSVTAILCFGVTVIA
                     IIADFIPFLGALICAVMLLSASAFLVVRFPSSRRGWDLRQTIEWIIGHPWDSARIGMF
                     PPLMATVPILSPFLVATMFPFLIIYGALNLDLIEGSRRKKTSVAQTMIVEEDS"
     gene            complement(3552..4001)
                     /gene="tsaE"
                     /locus_tag="PKH10_06955"
     CDS             complement(3552..4001)
                     /gene="tsaE"
                     /locus_tag="PKH10_06955"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNT27899.1"
                     /translation="MKERRQKFLSRSEEETERIGRGLAAALSPGTLITLEGALGSGKT
                     VLAGAIAEGRGVPRTQVQSPTYVILRQYTTGNILIYHWDLYRIATIDELETADFPELL
                     RDRSALTIIEWAGLFPSLWGHHAPRVEITLAEGADHAERRIDLLFVD"
     gene            complement(3998..>4843)
                     /locus_tag="PKH10_06960"
     CDS             complement(3998..>4843)
                     /locus_tag="PKH10_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNT27900.1"
                     /translation="SAEVPSNILSSLLEETDYLACDPAIDAHKGTNGRLLVIGGDASK
                     IGASFIAAEAFLASGGGLATVAVPDKHLLKLAGRYPGIMLTTTEEARAHPNRWDAILC
                     GPGLHNFSPELENFLFRARARLVLDAGIFDHADASFFKRFRQREILFTPHPGELARVM
                     ARMGTEGRWDVLVDAFPLGPRQVLYAKNAASMIRHKKQTVIVPHGARALSFGGSGDAL
                     AGIFASFALRFSDLLQAAVNGALLHRRAGILLETEFSPSFHRISNLIEQIGSAFSCFS
                     EETRL"
BASE COUNT         1206 a         1289 c         1418 g          930 t
ORIGIN      
        1 gaagaaaggt cccggcggtc actaccaccg ctcccgccgt cacctctccg gcggtccgta
       61 gaagcgcgcc cgttatgttc cctttttccg tcagcaaacc ggttgcctca tcctgccata
      121 tcgtaagcag gggaaaagaa agaagacggt cgttcaggaa ttttttgtac aaaagacggt
      181 cgttctgtga tcgggtcgaa cggaccgccg gtcccttgct ccggttaagc acgcgatact
      241 ggatcgccga aaaatcggcc gcctctccca tcaggccgcc aagcagatcg atctcgcgga
      301 cgatctgccc tttcgcctgt ccaccgatcg acgggttgca cgacatctcg ccgatcgaat
      361 cgatccgaag agtgaccagc agagtgcgcg ctccgagccg tgtagccgcg gccgccgcct
      421 ctatcccggc atgaccgccg ccgatgacga taatatcgaa taaattcata tacctccgtc
      481 gccgaccgcc atatttgccc tgtaacgcgc gaaaacggat cggcctaggc cttggtatgc
      541 atcataaata aaaagagcga aaagtaaaat ggagagaagg tcatttgccg agacagaacg
      601 acgagaatac ccgttcgtaa agatcggggt catcgctccg cccgacaagc tcgcccaaaa
      661 gggtccgaca gcgagagatc gcgacaacaa ggagcgcaag atccccggcg gaaaggagta
      721 agcgcaattc ttcaagcgcc gcgcaaagtc gttcggcgac cctccgtcgt tcggcatgaa
      781 gcaggatcgg caaagcggta tcagggagaa gagccgtgat ccgcccggca agaagagaga
      841 tcatgtccgg gagcccttcg ccggtaagag cggagatgaa aaggtcgggt gccatgctct
      901 cagccgcagg atgacgagcg atatccgctt tgttggcaaa gacgagaggt tttttcggcg
      961 caaaaagggc gcgggcggcg atatcagcgg cggtcggggc atcagaccca tcaaaaagaa
     1021 ggataatatg ctctgaaacc tcgctcaaag ttagagttcg cgtaattcca tctttctcga
     1081 tacccgacgc gatatcgcgc agaccggcgg tgtcgcacaa ttcgaccgtg acggcaggga
     1141 aaggaagagg ccacaaggcg gtcagataat cgcgcgtggt ccccggttcg ggagagacga
     1201 tcgcgcgatc aagaccaagg aggcgattga aaagaagcga ttttccgaca ttgggtcgtc
     1261 ccgcgatgag gatacgcggt gtcgcggcac gatcgaatcg ctgtgtgttc tcgagcgcag
     1321 gaatgaccag atgctcggtc tcttcgataa gaaccgatat ctttgcgaga ggaagatgtt
     1381 ccgtgacctg ttcgggaaaa tcgaccgtag cgttcgcgat agtgtccagt tcgtcccatt
     1441 gtgttaggag cgggaccgcc gcctgctcaa aaagtccgcc ataggcggcg cgccgcgcga
     1501 tatcggcctg caggcggtct tgggtcgcga tcagagcggg gatcgtggcg gcctgtgcga
     1561 gggtcatttt gccgttcacg acggcgcgga acgaaaactc gccgggtgtg gccggcgttg
     1621 caccaagttc ggtgagacgg gagagcgctt cctccatcgt gccagcacca ccatgaaggt
     1681 gaagttcgcc catctcttcg cccgtgtaac tcgccggagc ggcgaaataa aggactatcg
     1741 cctgttcaga agcggcgcga gggcccagat caagagaaca aagagttgct cggcgaggga
     1801 cgaaacgatc gcgaccggtc agttttttta ggacatcgac ggtcgtggga ccgcttatcc
     1861 ggatgatagc gatcgcgcca ccggtggcgg tcgcgggcgc cgcgatggta agtccgagtc
     1921 gctcggcaag tcgcacggga acctcctgtt gcccgccgca tgataccgat aacgagcctg
     1981 cttgcaacga tttcttgact cacgcttcga tctcggagac gatacgcaaa gaaaagagga
     2041 agaacctatg ctgttcgacg cccacataca cttaagcgag atcccgaagg ttgaacaggg
     2101 ccgcccgttg ccggcgtcgg tacttacctc ggcgcatgac ccggaagacc cgtctctttc
     2161 gctgggagac cccgcagtgc tttgttccta cggtctccat cccctctggc ttgatgctcc
     2221 gggacgttcg ttggaaacac tagtaaaact ttcagcttct ggtcagatat ccgcagtagg
     2281 ggagttcggg ttcgattttt atcatggtcg ggatagggaa aaggaaaaaa aacaaagaga
     2341 tttctttcat gcccaatgtg tcatcgctga acggtatcgt ctcccggtgg ttcttcatgc
     2401 cagacgcggg atggctgaac tgttcaatga aacgacgctc ctgaaaaaac tttccgcagt
     2461 aatattccat tgttatcccg gtactgctga ggagggtcag gccttcctga agcggggagt
     2521 gaacgggttt ttcagtttcg ggacaccgat cataaaaaac tatccgcgag ccatcgcggc
     2581 attgaagagg ctacccttgg atcgcatcct tttcgagacc gatgctccgt tccagccacc
     2641 gcatggtcgg gagatgtccc tccccatcga tatcgttacg gtctatgaaa aaggatcgga
     2701 attattgagt atacctcggc cagaacttga gcatatcgtg tggggtaccg cgcaaggcat
     2761 ctttcccggg agattatgaa tcctcctcca cgatcatcgt ctgcgcaact gatgtctttt
     2821 tacgccgaga cccttctatg agatcaagat tcagcgcccc gtagatgatg aggaaaggaa
     2881 acattgttgc tacaaggaac gggctcagga tcggtacggt ggccataaga ggagggaaca
     2941 tcccgatccg ggctgaatcc caaggatgac caatgatcca ctcaatggtc tgacgtagat
     3001 cccatccccg tctcgaagag gggaaacgga cgacaagaaa tgccgaggcg gaaagaagca
     3061 tcacggcgca gatcaaggcg cccagaaaag gtatgaaatc ggcgatgatc gcgatgaccg
     3121 tgacgccaaa gcaaagtatc gcggtgacgg agccatcctt gaggtcgccc aacacttcat
     3181 cgatgtcagg aaaggcgtca tccttcaact ttttaccatg gtacagggcc tcggccgata
     3241 tagaaaggaa ctgcagaaag ggggaggcga gcacataggc cggcaggaat gaaagaaaaa
     3301 aagaggcggc tcgaagacag acaaggaaca tgccgttcaa gaaccagaac agaaggtgcc
     3361 agagtggcga atcttcttcg gcgggggcgg gtatcacgaa ctgtatctgc gaagagacga
     3421 agttggtgaa aaaagagaga acgagaagca tgatcatgaa gaaaatcaga aagatgaaaa
     3481 gactccatat caggaggata cgccgggaac gatagaagag aagaaggtcc agtaacggta
     3541 caaaggaccg ctcagtcgac aaagaggagg tcgatccgtc gttcggcatg gtccgctcct
     3601 tccgcaagag ttatttcgac gcggggagcg tggtgccccc agagagaggg aaacaaccca
     3661 gcccactcga taatggtcag tgccgagcga tcccgcaaca gttcgggaaa gtcggctgtc
     3721 tccaactcgt caatcgtcgc tattcgatag agatcccaat gatatatcaa gatattgccc
     3781 gtcgtatact gacgaaggat aacataggtc gggctctgga cctgtgtacg gggaaccccc
     3841 cgaccttcgg cgatagcacc ggcgagcacc gtcttacctg aaccgagtgc tccttcaagc
     3901 gtgatgaggg ttcccgggga aagcgcggcg gcaaggcccc gaccgatccg ttcagtctct
     3961 tcctcggaac gggaaagaaa tttctgtcgt ctctccttca caggcgggtc tcctcggaaa
     4021 aacaggaaaa ggcggaaccg atctgctcga taaggttaga aatgcggtga aagctcggtg
     4081 agaattcggt ttcaagcagt atgccggcgc gtcgatgcaa aagcgcgccg ttcacggccg
     4141 cctgcagaag atcagaaaag cgcaaggcaa aggaggcaaa gatccccgca agggcatcac
     4201 cggacccccc gaaggaaagc gcgcgcgcgc cgtgaggaac gataaccgtt tgttttttat
     4261 ggcgtatcat ggatgccgcg tttttggcgt agagcacctg tcgtggtccg agggggaacg
     4321 cgtcaaccaa gacatcccat cgtccctctg ttcccatgcg tgccataacg cgtgccagtt
     4381 caccggggtg tggtgtaaag aggatctctc tctggcgaaa acgcttgaaa aaagaagcat
     4441 ccgcatggtc gaagatacct gcatccagca caagacgagc acgggctcta aataagaaat
     4501 tctccagttc aggagagaag ttatgaagac ccggaccaca aaggatcgcg tcccatcggt
     4561 tcgggtgcgc gcgggcctct tcggtcgtcg tgagcatgat gccggggtat cggcccgcca
     4621 gtttcagaag gtgcttgtcg gggactgcga cggtcgcaag tccgccgcct gacgcgagga
     4681 acgcctcggc ggcaatgaaa gaggcaccga tctttgaggc atctcccccg atgacaagta
     4741 gccgtccatt cgttcctttg tgtgcgtcga tagccggatc gcaggcgaga taatcagttt
     4801 cttcaagcag agacgacaaa atattagacg gcacctccgc cga
//
LOCUS       DAOQUE010000193         4842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_19425_length_4842_cov_4.508878, whole genome shotgun sequence.
ACCESSION   DAOQUE010000193 DAOQUE010000000
VERSION     DAOQUE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19425_length_4842_cov_4.508878"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PKH10_06965"
     CDS             <1..392
                     /locus_tag="PKH10_06965"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT27901.1"
                     /translation="DGRKYPWGNTPEASCDYAVMDDSNAGGNGCGTDGTMPVGSKPNG
                     VSPYGAYDMIGNVWEWVNDWYASDYYASSPTNDPTGPETGANRVLRGGAWNRYSDINL
                     RASYRNLDPPEYYYLDYRGFRCAMDAE"
     gene            409..1443
                     /locus_tag="PKH10_06970"
     CDS             409..1443
                     /locus_tag="PKH10_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27902.1"
                     /translation="MTKYLMILVAAMLIVACGDEGTKGPAMGSKGGDCYGNGTCDGDL
                     VCMSNVCVDPTETPDKDTAVNDEAPDDELDELTDVDDEQIVDNEEDDTDSLVNDDDKL
                     DPEIAEEFNKPCYNPFIVAEIFAKKCVEYTKYTVNGLECGITVNMTEVGPMQTLECTT
                     KEKLDKLKEQIIEDKPENWIDSTKLEVYNQVSKLYFSEKYGEISYKLIDKPDPILSNN
                     DTVEGYITEFTFGGKDFNIVDVYRDKDTIPLIISDKNGTQAELIENAVIEIYDVNYPI
                     LTIQWEYGYCFYPPKDNLTRDFGVMGTYVLVNAKKAYDETNAKNEANQCDKVHEEQFE
                     EYKEKVKKEN"
     gene            complement(1596..2972)
                     /locus_tag="PKH10_06975"
     CDS             complement(1596..2972)
                     /locus_tag="PKH10_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27903.1"
                     /translation="MKKTLFDIGLLVYGAAAYFGYLVVAVFVLKDHYDIGLYLFSVPF
                     LYAIFRVLSARIKRASLLTAIYLALPFFVSVYLSLTGRPAVWEITGILPVTVLLSYSL
                     RSEKSSSPLFTRLLFPVWALFFLLVLFLPTVNYLNNRDLPRALQLLLSIYTPAVTLST
                     LAVFLRFVIETASLNMALNNFRLFIHGARIDRVVFDSADFLSLSQTNLSGIVTGPAMS
                     REQFLKKVDLLNREFVSTEGGVDAMKRDAKFSRTLPDGTELTLAPLALLIREGFLTDD
                     LPPAAEDPKRSYVALAEGRRVIGYYVVDRFDPTANRTMIDVLQEKYTVPVVIVGGEEL
                     KKRFPGALIVPRAGDITLDNRDLLVTQEPPENSPAIVMAWGKKNVDAGDIFLADPAML
                     NIMRLVLVLGGVRGRLYRSIAVSSIPFLLPLFLSIYRLHIPQINAVAVLIMILLTMTQ
                     TFRSMKAE"
     gene            complement(2992..3609)
                     /locus_tag="PKH10_06980"
     CDS             complement(2992..3609)
                     /locus_tag="PKH10_06980"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNT27904.1"
                     /translation="MQRIPVHPRTPHKRTVETVADALGSGGVALIPGDTSYLLAVRIG
                     KKSAIEKLGRIKDRKKKFYSVMFRDFSELARYAEVTDRQFATVKRFLPGPYTFILKAS
                     REVPKIMLENRKEIGIRIPDLPFIRELIAACGEPLIVSTAKGGNDEEEEFFTDPDSET
                     PPWLHLVDIIADAGYQPAELTSIIRLENDRYEVVRAGKGPVEEFS"
     gene            complement(3614..>4842)
                     /locus_tag="PKH10_06985"
     CDS             complement(3614..>4842)
                     /locus_tag="PKH10_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HNT27905.1"
                     /translation="LGPTSETEALRKVSLLFSCGEPPTRDDLAHIPRYEQLKRLAENY
                     RNNPAGALFILQHYANEKDPVAVETLLHLLIDEQRIDEAETLVAEIRGSAAATSDIVQ
                     SLILRVEKRNTEEKELLRRALLPEISRGEPYPALRLMEMAGADNQRARVFLQNLLAAL
                     GVPGYRPAEQEWQRLSALREELILAQANFRPLDGKLRSLAQLLAQARVAERKEERRFE
                     PLSSSTHRDILTLLEKLSLALRIDPPSAFFDPSLIGPLTIGLANLPVLVFGKGATVVD
                     RKRLAFLIATNLFLIGSGVFDGHQEKGVTALTARLGEILAMPDKARAPFVRTLKKKQQ
                     PDVTAALAELDGTSEQDIAGFGERLYLASLLYAFSVIPDIGVVFALNGESKGNLDAPS
                     SRSAQMFDFALNAFLG"
BASE COUNT         1170 a         1129 c         1539 g         1004 t
ORIGIN      
        1 ccgatggacg gaaatacccg tggggtaata cgccggaagc gtcatgcgat tatgcggtca
       61 tggatgactc gaacgccggc ggcaacggtt gtggaaccga cggaacaatg ccggtgggga
      121 gcaagccgaa tggcgtatcg ccctacggtg cttacgacat gatagggaat gtgtgggaat
      181 gggtgaacga ctggtatgcc tcggattact atgcttcgtc gcctacaaac gaccctacag
      241 ggccagaaac tggtgctaac cgcgtgttgc gcggtggggc gtggaaccgc tacagcgaca
      301 tcaacctgcg ggcgtcctac cgcaaccttg atcccccaga atactactac ctcgattatc
      361 gcggattccg gtgcgcaatg gatgctgaat aaaataaggg aggaaactat gaccaagtat
      421 cttatgatcc ttgtggcggc aatgttgata gtcgcctgtg gcgatgaggg gacgaaaggc
      481 ccggcgatgg ggtcgaaggg gggtgactgt tacgggaacg gtacctgcga cggcgacctc
      541 gtctgcatgt ccaatgtgtg cgttgatccg accgaaacgc cggacaagga cacggctgta
      601 aatgatgaag caccggatga tgaactggac gagttgacag atgttgatga tgagcaaatt
      661 gttgataatg aagaggatga tacggattca ttagtgaatg atgatgacaa attagatcca
      721 gaaatcgcag aagaattcaa taaaccttgt tataatccct ttatagtcgc agaaatattt
      781 gcaaaaaaat gtgttgaata cactaaatat actgttaatg gtcttgaatg tgggataacg
      841 gttaacatga ccgaagttgg cccaatgcaa acgttggaat gtacaacaaa agaaaaatta
      901 gataagttaa aagaacaaat aatagaagat aaacccgaaa attggataga tagtactaaa
      961 ttggaagtat ataatcaagt ttccaaactt tatttctcag agaaatatgg tgaaatatca
     1021 tataaattaa tagataaacc cgatccaata ctttctaaca atgatacagt tgaaggatat
     1081 ataacagaat tcacgttcgg agggaaagat ttcaacatag tagatgtata cagagataaa
     1141 gatacaatac cactaataat ttctgataaa aacggaactc aagcagaatt aattgaaaat
     1201 gcggttattg aaatatatga cgttaattac ccaattttaa ctatacaatg ggaatatggt
     1261 tactgtttct accccccaaa agataatcta acaagagatt tcggtgttat gggaacatat
     1321 gttttagtca atgcaaaaaa agcatacgat gaaactaatg caaagaatga agcaaatcaa
     1381 tgtgataagg ttcatgagga acaatttgaa gaatataaag aaaaagttaa aaaagaaaat
     1441 tagttttttt caagggggat tgagggggtt tgccccattc cgacgcgttg aaaacccacc
     1501 cagccctccc ttgacagggc gggatagcgt cgtcccgctg tcaaggggga acgagggggt
     1561 ttgtgcgatc caaggcggcg tagccgggtg aggtattatt ccgctttcat gctccggaag
     1621 gtctgcgtca tggtgagcag gatcatgatg aggaccgcga ccgcatttat ctgcggtata
     1681 tgcagccggt atatcgaaag gaacagcggc aacaggaagg ggatcgacga cacggcgata
     1741 ctgcggtaga ggcggcccct gacgccgccg agcacgagca cgagccgcat gatgttgagc
     1801 atcgcggggt cggcgaggaa gatgtcgccg gcgtccacat tcttcttgcc ccatgccatg
     1861 acgatggcgg ggctgttctc cggcggttcc tgcgtgacga gcagatcgcg gttgtcgagc
     1921 gtgatgtccc cggcgcgcgg gacgataagg gcgccgggga accgcttctt gagttcttcg
     1981 ccgcctacga tgaccaccgg taccgtatat ttttcctgca ggacatcgat catcgtccgg
     2041 tttgcggtgg ggtcgaagcg gtcgaccaca tagtagccga tgacgcggcg tccttccgcc
     2101 agtgccacat aggaccgctt ggggtcttcg gcggcgggcg gcaggtcgtc ggtcaggaaa
     2161 ccctcacgga tgagaagggc gagcggcgcg agcgtcagtt cggtgccgtc ggggagcgtc
     2221 cggctgaatt tcgcatcccg cttcatggcg tccacgccgc cttcggtcga tacgaactcg
     2281 cggttgagga gatccacctt tttcaggaac tgttcgcgtg acatcgccgg gccggtcacg
     2341 atgcccgaaa gattcgtctg cgagagcgaa aggaaatcgg ccgaatcgaa caccacccgg
     2401 tcgatgcggg cgccgtgtat gaacagccgg aaattgttga gcgccatgtt gagactcgcg
     2461 gtctctatca cgaagcggag gaacaccgcg agggtggaga gggtgacggc gggagtgtag
     2521 atggagagga ggagttggag cgcgcggggc aggtcgcgat tattgaggta gttgacggtc
     2581 gggaggaaca gtacgaggag gaagaagagc gcccagaccg gaaagagcag tcgcgtgaag
     2641 agcggcgaag aactcttttc ggagcgcaga gaataagaca ataagacggt gaccggtagt
     2701 atcccggtga tctcccagac cgccggccgc ccggtcagcg acaggtagac cgatacaaag
     2761 aaggggagag caagatagat cgcagtgagg agcgacgccc gttttatccg ggcgctgagg
     2821 acccggaata tggcgtaaag gaagggaacg gaaaaaagat agagtccgat gtcataatgg
     2881 tccttgagca cgaaaacggc gacgacgagg tagccgaaat aggcggcggc gccgtagacg
     2941 agcaggccga tgtcgaacaa ggtctttttc atcggttgct tccgggatgc gtcatgagaa
     3001 ctcctcgacc ggtcctttgc cggcgcggac gacctcgtaa cggtcgttct ccaaccggat
     3061 gatgctggtc agttcggcgg gctgataccc ggcgtcggcg atgatgtcca cgagatggag
     3121 ccagggcggg gtctcgctgt ccggatcggt gaagaattcc tcctcttcgt cgttcccccc
     3181 cttggcggtc gagacgatga gcggctctcc gcacgcggcg atgagttcgc gtatgaaggg
     3241 gagatcgggg atgcgtatgc cgatctcctt gcggttctca agcatgatct tgggcacctc
     3301 gcggctcgcc ttgaggatga aggtgtaggg acccggaagg aatcgtttga ccgtggcgaa
     3361 ctgccgatcg gtgacctcgg cgtagcgggc gagttccgaa aagtcgcgga acatgaccga
     3421 atagaatttc ttcttacgat ctttgatgcg gccaagtttt tctatggcgc ttttctttcc
     3481 gatgcggacg gcgagcaggt aggaggtgtc accggggatg agcgcgacac cgcccgaacc
     3541 gagcgcatcg gcgacggtct ctacggtccg tttgtgcggg gtacgaggat ggacggggat
     3601 gcgttgcatg gcgttatccg agaaaagcgt tgagggcgaa gtcgaacatc tgtgcggagc
     3661 gggaagaggg ggcgtcgaga tttccctttg actcgccgtt cagggcgaac accacgccga
     3721 tgtcaggtat aaccgagaag gcataaagca gagatgcgag gtagagccgt tcaccgaagc
     3781 cggcgatatc ctgttcggag gttccgtcca gttcggcgag cgcggcggtc acgtcgggtt
     3841 gctgtttctt tttcagcgta cggacaaagg gggcgcgggc cttgtcgggc atggccagta
     3901 tctcgccgag tcgcgcggtg agtgcggtaa cgcccttttc ctgatgaccg tcgaagacgc
     3961 cactgcctat cagaaagaga ttcgttgcga tgaggaacgc cagtcgcttg cgatcgacga
     4021 cggtcgcgcc tttaccgaac acgagaaccg ggaggttggc gagtccgata gtaagggggc
     4081 cgatgagcga cgggtcgaag aaggccgacg ggggatcgat cctcaaagca agcgagagtt
     4141 tttcgagcag ggtgagaatg tcgcgatgtg tcgaggagga gagcggttcg aaccgccgtt
     4201 cctccttccg ttcggcgacc cgcgcctgcg caagcagttg cgcgagcgaa cggagtttgc
     4261 cgtccagagg ccgaaagttc gcctgagcga gaatgagttc ttcgcggagc gccgacagcc
     4321 gttgccattc ctgttcggcg gggcgatatc ccggcacacc gagcgcggca agcaggttct
     4381 ggaggaatac ccgcgcgcgc tggttgtcgg cgccggccat ttccatgagt cgcaaggcgg
     4441 ggtagggttc tccgcgcgag atttcgggga gaagggcgcg gcgcaggagt tccttttcct
     4501 cggtgttcct tttctcgacg cgcagaatga gcgactgtac gatgtccgat gttgcggcag
     4561 cgcttccgcg tatctcggcg acgagcgttt cggcctcgtc gatccgttgc tcgtctatca
     4621 agagatgaag cagcgtctcg accgcgacgg ggtctttctc gttggcatag tgttgaagga
     4681 tgaacagggc gccggccggg ttgttacgat aattctcggc aagtcgtttg agttgttcgt
     4741 agcgggggat gtgtgcgagg tcgtcgcgtg tcggcggttc accgcaggag aagagaagcg
     4801 agaccttgcg gagcgcctcg gtctcggagg tcggacccaa gg
//
LOCUS       DAOQUE010000194         4832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_19483_length_4832_cov_4.342474, whole genome shotgun sequence.
ACCESSION   DAOQUE010000194 DAOQUE010000000
VERSION     DAOQUE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19483_length_4832_cov_4.342474"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="PKH10_06990"
     CDS             complement(<1..1719)
                     /locus_tag="PKH10_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27906.1"
                     /translation="MSSKGMFENRVAFLLPFLCSLFLLFACGGAEGTVENGIQPLVEV
                     SPGIDGKPTADAKVVAPFDLGTVIRQVKYAFRAEGDTFTGGDLGYAVAAQKGGAFTVT
                     PRHEPMGELAVTGAPVRFETVSLSDGAVTTAQDGHLTIARGAVTEHLRNSEDGVEQSW
                     AFAARPTGTDDLVIRVAVSGMAYIGETEKGLHFAEESGIGVRYGHAKWIDNDKKETAI
                     AAVFRDGHIELTVPRDLIDGSSFPAVLDPGIGAEFGMDNPVAVPNTGTNYYPAVAFDG
                     TNYLVVWQDSRSGTYDIYGARVAKNGAVLDPTGIVISNAANQQYYPAVAFNGTDYLVV
                     WEDLRSGSTYDIYGARVAKNGTVLDTGGIVISNATNNQEWPSVASDGTDFMVVWHDYR
                     SGSTYDIYGARVTAAGVVSDPSGIVVSNVANQQIYPAIDHDGTNYLVVWMDLRSGGSY
                     DIYGARVASGGTVLDTTGIMISNAANSQEVPALAFNGTNYLVLWHDSRSGGLDIYGAR
                     VATNGTVLDAAGFTISAAANSQMYPAVASDGSGYFVAWMDVRNGSNYDIYGARIDTDG
                     NVLDVSG"
     gene            complement(1815..3566)
                     /locus_tag="PKH10_06995"
     CDS             complement(1815..3566)
                     /locus_tag="PKH10_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT27907.1"
                     /translation="MKVCIGALVLFISVSLLAVDACYYGAGKKFCMTRVDDTVSYRGG
                     APMKLTGELGIRFADGTSEAARRKLLSLYLLSAVGDIEGLPQYVRARPAPGGDVFAIS
                     RRLVESGRVKWAQPVWYERPHLLATTPNDTYYGEAWHHGMIHSPEAWDIVTGDPRVVL
                     GIVDCGTDTQHPDLFSHMLMGNSFASNEPYVDPDPSGTNLTAYSRAHGTAVAGVATAT
                     GNNGFGVVGVCWDCGLLPVKYFGNEMIIPNDRKLAALTWTVDNGAWVINNSWLINQDK
                     DGDTCKLNIPYDNYAGEAVAYGKTNGRGGRGTVMVFGAGNSACDTQWNDNFKNLHGDI
                     LVVSALRSNGEITDYSNYGIYIDISAPSGFDTVDNKGLVTTDWTVAGMGYNPHFMNDL
                     PDQSYTKFFNGTSGAAPVVTGAIGLMLSVAPYLTHAEVIACVKQSAAAGTQGCIHGSD
                     EECYGAGVLDVQAMVENAKNGLCGGTPECDGPEDCGEGECWNGICVEAGTVTDDDVMP
                     DDTVPDEAVTDDAVEDDVDATTGADDMVQPDQPTVDDTPVTDEPTPDADGQNDETPGT
                     DAIDPVNDDNGCGCSLL"
     gene            complement(3594..>4832)
                     /locus_tag="PKH10_07000"
     CDS             complement(3594..>4832)
                     /locus_tag="PKH10_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27908.1"
                     /translation="DGLFYVVLPYQAPEDVTRTLLFSTDFSRVDIGIMLDLSGSMEQE
                     LNNLRDEIQTKIVEGIPAELPGVDIAFGFLHFQHYQQNLMFQVDQMITTDGAKVVTAV
                     NQYTADDVWGGVEPMVEVLYQASTGEGLTANYLQEASFGGGYTTNAINLPPTDCTGEA
                     GTIGGMCFRTFALPIFIMITDEKFPVIGIQGTASCPAQSSYFQDPEGCWDPNFMGHSF
                     DEAISAMNSVNAKFIGIDSGFKCDGTEDENGNCDSTYSPTDEAKNDFLMVADATASLD
                     GNGTAFLYHTADSTGNGLSDQIADAVKQLTTFIEKDINTVAESDESCNDISAAAFIVS
                     SKPNKADPDNGYASKDDTTFYKVQPGTEVYFDVHFHNDFCRNTELQPKVYKARIRVLG
                     EGAFLSSREVQVIVPGSQAE"
BASE COUNT          939 a         1517 c         1368 g         1008 t
ORIGIN      
        1 ccccgacaca tcaagcacat tgccgtcggt atctatccgg gcaccgtaga tgtcgtagtt
       61 gctgccgttc ctgacatcca tccacgccac gaaataaccg ctcccatcgg atgcgaccgc
      121 cggatacatc tggctgttgg ccgccgccga tatggtgaat ccggccgcat cgagcaccgt
      181 cccgttcgtg gcgacccgcg cgccgtagat atcaagaccg ccactccggg aatcgtgcca
      241 taacacaaga taattcgttc cgttgaaggc aagggccggc acttcctgac tgttggcggc
      301 attggatatc atgatgcccg tggtatcaag caccgttccc ccagaagcca cccgtgcgcc
      361 gtagatgtcg taggaacccc cgcttcgtag atccatccac acaaccaggt aattggtccc
      421 gtcatggtcg atcgccggat atatctgttg gttcgccaca ttcgacacca cgatgccgga
      481 ggggtcggaa accacgccgg cggccgtgac ccgcgcaccg tagatgtcgt aggtgcttcc
      541 gctccggtaa tcgtgccaca ccaccatgaa atcggtccca tcggaggcga ccgacggcca
      601 ttcctgattg ttggtcgcgt tcgatatcac gatgccgcca gtatcaagta ccgtcccatt
      661 cttggcgacc cgcgcaccgt aaatgtcata agtgctacca ctccggagat cctcccacac
      721 gacgagatag tcggtcccgt tgaaggcgac cgccggataa tattgctgat tggccgcgtt
      781 cgatatcacg atgccggtcg ggtcaagcac cgccccgttc ttggcgaccc gcgcgccgta
      841 gatgtcataa gttccgctcc ggctatcctg ccacaccacg agatagttgg tgccgtcgaa
      901 ggcgaccgcc gggtagtagt tcgttccagt attcggcacc gccaccgggt tatccatgcc
      961 gaactcggcg ccgatgccgg gatcgagcac cgccgggaac gatgaaccgt cgatgagatc
     1021 gcgcggcacc gtcagttcga tgtgcccgtc gcggaacacc gccgctatcg ccgtctcttt
     1081 cttgtcgttg tctatccact tcgcatgacc gtagcgcacc ccgatgccgc tctcttcggc
     1141 gaagtgaagc cccttttcgg tctcgccgat gtaggccatc cccgacaccg cgacccggat
     1201 gacgagatca tccgttccgg tgggacgtgc cgcgaatgcc caactctgct ccacgccatc
     1261 ttccgaattg cgaagatgct cggtgaccgc gccacgcgct atcgtcagat gaccatcctg
     1321 tgccgttgtc acggctccat cgctcagcga aacggtctcg aaccggaccg gcgcgccggt
     1381 gacggcgagt tcgcccatcg gctcatggcg cggcgtcacg gtgaaggcgc cgcctttttg
     1441 cgccgcgacc gcgtagccga gatcgccacc ggtgaaggta tcgccctccg ccctgaaagc
     1501 atatttgacc tgccggatga ccgtgccgag atcgaacggc gccacgacct tcgcatcggc
     1561 cgtcggtttt ccatcgatcc ccggagagac ctccacgagc ggctggatac cgttctccac
     1621 cgttccttca gcgccgccac aggcgaaaag aaggaaaagg gaacaaagga acgggagcaa
     1681 aaacgccacc cgattctcga acataccttt gctactcatg ggaagatcct cctcatttgt
     1741 tggcaagcac ccttcgataa ggtcccctat tgaatcacca tttgaacgat ttgtcaaaaa
     1801 gtatggggaa ataatcagag aagcgaacag ccgcagccat tgtcgtcgtt caccggatcg
     1861 atggcgtcgg tgccgggcgt ctcatcgttc tgtccgtcgg cgtcaggggt cggctcgtcg
     1921 gtcaccggcg tatcatcgac ggtcggctga tcgggctgaa ccatatcgtc cgctccggtc
     1981 gttgcatcca catcgtcctc gaccgcatca tcggtcacgg cctcatcggg cacggtgtcg
     2041 tcaggcatca catcgtcatc ggtgaccgtt cccgcctcga cgcagatgcc gttccagcac
     2101 tccccttcgc cgcaatcctc gggaccatca cactccggcg ttccgccgca caggccgttc
     2161 ttcgcgttct ccaccatcgc ctgcacatcg agtacgccgg cgccgtaaca ttcttcgtcg
     2221 cttccgtgta tacagccctg cgtaccggcg gcggccgact gtttcacgca ggcgatgacc
     2281 tcggcatgcg tcagataggg ggcgaccgac agcatcagcc ctatcgcacc ggtcacgacc
     2341 ggagcggcac ccgaggtgcc gttgaagaac ttggtgtaac tctggtcggg cagatcgttc
     2401 atgaagtgcg ggttataccc catgccggcg acggtccagt cggtggtcac gaggcccttg
     2461 ttgtcgacgg tgtcgaagcc ggaaggcgcc gagatgtcga tatagatgcc gtagttcgag
     2521 tagtcagtga tctcaccgtt ggaacggagt gccgacacga cgaggatgtc gccgtgcagg
     2581 ttcttgaagt tgtcgttcca ctgcgtatcg caggccgaat tgccggcccc gaagaccatg
     2641 accgtgccgc ggccgccgcg accattcgtt ttaccgtagg cgaccgcctc gccagcgtaa
     2701 ttatcgtacg ggatgttcag tttgcaggta tccccgtcct tgtcctgatt gatgagccac
     2761 gaattattga tgacccacgc gccgttgtcg accgtccagg tgagcgccgc gagtttgcgg
     2821 tcgttgggga tgatcatctc gttgccgaag tacttgaccg gcaagagtcc gcagtcccag
     2881 cagaccccca cgacaccgaa cccgttgttc ccggtcgccg tggcgacccc ggccaccgct
     2941 gtgccgtgag cccgcgaata ggcggtaagg ttcgtcccgc tcggatcggg atcgacatag
     3001 ggctcgttgc tggcgaaact gttccccatg agcatatggg agaacaggtc gggatgttgc
     3061 gtatcggtgc cacaatcgac gataccgagc accacgcgcg ggtcgccggt cacgatatcc
     3121 cacgcctcgg ggctgtgtat cataccatga tgccatgctt cgccgtagta ggtatcgttc
     3181 ggtgtggtcg caaggagatg cgggcgttcg taccataccg gttgggccca tttcaccctg
     3241 ccgctctcga caagccgccg ggatatcgcg aacacatcgc ctcccggtgc gggccgcgcc
     3301 ctgacatact gcgggagccc ctcgatatcc ccgaccgcgc tgaggagata cagcgagagg
     3361 agtttcctcc gcgccgcctc cgatgtcccg tcagcgaaac ggatacccag ttcaccggtg
     3421 agtttcatcg gcgcaccccc gcggtaggag acggtgtcgt ccacgcgggt catgcagaac
     3481 ttcttgccgg caccataata gcaggcgtcg accgcaagaa gcgagaccga tataaaaaga
     3541 actaaagcgc ctatgcatac tttcatcgcc ttcctccgca taactgttcg tttttactct
     3601 gcctgcgagc cggggacaat gacctgcacc tcgcgggacg agaggaatgc accctcgccg
     3661 agcacccgta tccgcgcctt atacaccttg ggttgcaact cggtattgcg gcagaaatcg
     3721 ttgtgaaaat ggacatcgaa atagacctcg gtgccgggct ggaccttgta aaaagtggta
     3781 tcgtccttgc tcgcgtagcc gttatccgga tcggctttat tcggttttga cgaaacgatg
     3841 aaggcggcgg cgctgatatc gttacacgac tcatccgatt cggcgacggt atttatgtcc
     3901 ttctctatga aggtggtcag ttgtttcacc gcatcggcta tctggtccga aaggccgttg
     3961 ccggtcgaat cggcggtatg gtagaggaac gcggtgccgt tgccgtcaag cgaggcggtc
     4021 gcgtcggcca ccataagaaa atcgttcttg gcctcgtcgg tcggcgagta ggtcgaatcg
     4081 cagttgccgt tctcgtcctc cgtgccgtcg cacttgaagc cggaatcgat gccgatgaac
     4141 tttgcattga cgctgttcat cgcactgatc gcctcgtcga acgagtgccc catgaaattc
     4201 gggtcccaac atccttcagg atcctggaaa taactggatt gcgccggaca actcgccgtg
     4261 ccctgtatgc cgatgaccgg gaacttctcg tcggttatca tgatgaatat cggcagtgcg
     4321 aaggtgcgga aacacatgcc gccgatggta cccgcctcac cggtacagtc ggtcggtggc
     4381 aggttgatgg cgttggtcgt gtaaccgccg ccgaaagatg cttcttgtag atagttcgcc
     4441 gtcagccctt cgccggtcga cgcctgataa agcacctcca ccatcggctc gacgccgccc
     4501 caaacatcgt cggcggtgta ctggttgacc gcggtcacga ccttcgcgcc atcggtcgtt
     4561 atcatctgat cgacctggaa catcaagttc tgttgataat gctggaaatg gaggaacccg
     4621 aaggcgatat cgaccccggg caattccgcc ggtatcccct cgacgatctt cgtttgtatc
     4681 tcgtcgcgta ggttgttgag ttcctgctcc atcgagcccg acaggtcgag catgatgccg
     4741 atatccaccc gcgagaaatc ggttgagaac agaagcgtcc gggtcacatc ctcaggcgcc
     4801 tggtagggta acacgacata gaaaagcccg tc
//
LOCUS       DAOQUE010000195         4801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_19663_length_4801_cov_3.983565, whole genome shotgun sequence.
ACCESSION   DAOQUE010000195 DAOQUE010000000
VERSION     DAOQUE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19663_length_4801_cov_3.983565"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="PKH10_07005"
     CDS             complement(<1..1130)
                     /locus_tag="PKH10_07005"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNT27909.1"
                     /translation="MERSIPCGILDTDTADHALPEIDEMLDTDDVPDEEPDGWPEFEN
                     DEDVPREPFIVQWGSVANEAGVAAVAAVDGAVYVTGGTTGDIGSNSGQGSSDTFLSKL
                     GPSREVLWTRQWGTSNFDQGLTLTMGSVGEVYLAGSRDGVVDPFTGAGIGQMFISRFT
                     KDGEEEWTREWGSLSQGYQEYPASATVDRQGDIYVTGWTYGTIGAAGNQGLSDIFLSR
                     FAPDGAERWSVQWGSGLIDVGYGVVAAGEAGLYVTGWTYGKLDLDRSVFGGNDVFLAR
                     VNASGGVLWTRQWGSYEEDKSFAIAVGSDGGVFLAGQTSGSLGSEFPNKGLSDNFLVK
                     YSASGERLWLYQWGSAGNDVAYAVAVAPDGKVYVSGYTEGSLG"
     gene            complement(1199..2230)
                     /locus_tag="PKH10_07010"
     CDS             complement(1199..2230)
                     /locus_tag="PKH10_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27910.1"
                     /translation="MTRFEKNGKTGTIATAVSNSVLALLFVFLYPVVSFAERFWAEGT
                     IGTLFDSNIEHLSIVDLLTMGERRGVFLRNAALGGVAFGRDDAFSASVSLAAETGLDV
                     PERSRLNSYADVGWHLEPRSDVSLDLVFGLHHTAEDFLSMRNMFLDAFITGDLFWDIE
                     DAHAWYGALKTGYYLGFDEEMRYLTGPMFAFESGYFHYPTDATDYVKPSVGVEGYFFR
                     PEVLSSCEGTLSVNNSFIKPTAGFEGKYDLAPVFFKGSLRYSFMAWLASDRWWGGRSK
                     RRMEHVPSANGQVLWEITEHFALSLNYSYRYIFSNLGSEEGDYIDYTMDRHLVTLQIT
                     GRYEGERKK"
     gene            complement(2172..2714)
                     /locus_tag="PKH10_07015"
     CDS             complement(2172..2714)
                     /locus_tag="PKH10_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27911.1"
                     /translation="MGRLTSACLLLFLPLICLSKAVPELKSTVRILEGVVAPDWEERF
                     LPPAEPSATLTTPRESADADILNTTPPLPRKTRAVTPVKNDRRPVADLIRPDPEAEPS
                     PSPRQEDAPRSRAVEKIVKGAVAPAHHERTRQTTVPPVSKIDKEEETGDDSIEKRKKE
                     RRDTLREERKDRNYRNRRIE"
     gene            complement(2698..3402)
                     /locus_tag="PKH10_07020"
     CDS             complement(2698..3402)
                     /locus_tag="PKH10_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27912.1"
                     /translation="MGWLGRALDPAAEQDLREHCARCRSCAEEFAIAQKMAQGMADLK
                     ARPVAIPEDFDATLHIKLVERQQGRSREGKGRSLWGGLRWGVAALGIVVALAVVLIAV
                     RQGPSSEEEGVLISRAEVSAGRPITIELAYEASQDIEQVTVTIDLDEGLSFHSSVPEI
                     SSRRMLAWTGPLVKGSNTIPFVVTVDRVGVREIRTSASYEGAVHRHRVVLAADGLTVV
                     IAQYRLRSEVVHGSLN"
     gene            complement(3476..4039)
                     /locus_tag="PKH10_07025"
     CDS             complement(3476..4039)
                     /locus_tag="PKH10_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT27913.1"
                     /translation="MKQERYAELVRRSIRGEVAAFEELIGKYKQGLYAYLFGMTGGDH
                     AAAADILQEAFLKAFLNISRFRGEASFMTWLWRIARNEFIDYCASPKTSKNLSVDDLP
                     EKELAQESDFRDSLHQRDRGEHLRRLIGSLSPEHREVLELVDLQELRYDEAARIVGIS
                     EGALKSRLFRARERLFALVLENRELFS"
     gene            4159..4719
                     /locus_tag="PKH10_07030"
     CDS             4159..4719
                     /locus_tag="PKH10_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNT27914.1"
                     /translation="MNLTETLLLATSLSMDAFAVAIAAGVGSPRADRRVAGTIALFFG
                     GFQCLMPVIGFFVAGAFYGYICHFDHWIACGLLVLIGGRMIYEAATGDGCPRSVDLGP
                     RSLLVLALATSIDALAAGVGLTFLCSSIWLPALTIGVVTFAFSFVGFLFGRRLGCAFG
                     RKMEILGGLTLCLLGLKILGEGLIDG"
BASE COUNT          948 a         1544 c         1370 g          939 t
ORIGIN      
        1 ccgagcgatc cttcggtgta gcctgagaca tacacctttc cgtcgggagc cacggcgacg
       61 gcataggcga catcgttacc cgcgctcccc cattgataaa gccagagtcg ctcaccggat
      121 gccgagtatt tgacgaggaa gttgtccgac agacctttgt tggggaattc gcttcccagg
      181 ctcccactgg tctgccccgc gagaaatacc ccgccgtcgg accccaccgc tatcgcaaag
      241 gacttatctt cttcgtacga cccccactgt ctcgtccaga ggactccccc ggaggcgttg
      301 acccgcgcga ggaagacatc gttcccgccg aaaacgctcc gatcgagatc gagcttcccg
      361 taggtccagc cggtcacata gagcccggcc tctcccgccg caacgacacc gtacccgaca
      421 tcgatgagtc cgctccccca ctggacgctc cagcgttcgg cgccgtcggg agcgaaccgg
      481 gaaaggaaaa tgtcgctcaa cccctgattg cccgccgcac cgatcgttcc gtaggtccaa
      541 ccggtcacat agatatcgcc ctggcgatcc acggtcgccg aggcggggta ttcctgatat
      601 ccctgcgaca gagaacccca ctcgcgggtc cattcttcct caccgtcctt ggtaaagcgg
      661 gaaatgaaca tctgaccgat accggcaccg gtgaagggat cgacgacacc gtcacgcgac
      721 ccggcgaggt agacctcgcc aaccgaaccc atcgtgaggg taagtccttg atcgaagtta
      781 ctcgtccccc actgccgcgt ccagagaacc tctcgtgacg gacccagttt agaaaggaat
      841 gtgtcgctcg aaccctgccc gctgttgctg ccgatgtcgc ccgtggtacc gccggtcaca
      901 tagaccgccc cgtcgaccgc ggcgaccgcc gcgacccccg cctcgttcgc cacggacccc
      961 cactggacga tgaacggctc gcgtggcaca tcctcgtcgt tctcgaactc gggccaccca
     1021 tcgggttcct cgtcgggaac atcatcggtg tcgagcatct cgtcgatctc cggcaaggca
     1081 tgatcggccg tgtcggtatc gagtatgccg cagggtatcg aacgttccac cggtcccgca
     1141 caagacaggg ccagcatgag ggacaggagc cccggaaacg tcacggccaa ggatcgtctc
     1201 atttcttccg ttctccttcg taccgtcccg ttatctgaag cgtgaccaga tgacggtcca
     1261 tcgtgtagtc gatgtagtcc ccctcctcac tgccaagatt cgagaatatg taccggtagg
     1321 aataattgag cgacaaggcg aaatgttccg ttatctccca gagcacctgc ccgttcgccg
     1381 aagggacatg ctccatgcgg cgtttggagc ggccacccca ccagcggtcg ctcgccagcc
     1441 aggccatgaa tgaatagcgc agagagccct tgaagaacac gggagcgaga tcgtacttgc
     1501 cctcaaagcc cgccgtcggc ttgatgaacg agttgttgac cgagagggtc ccctcgcatg
     1561 atgaaagcac ctcgggccga aagaagtatc cttcaacgcc gacggaaggt ttgacatagt
     1621 cggtagcgtc ggtcgggtaa tggaaatagc cgctctcgaa cgcgaacatc gggccggtca
     1681 gatagcgcat ctcctcgtcg aaacccagat agtaccccgt cttgagcgca ccgtaccacg
     1741 catgtgcatc ctcgatatcc cagaacaggt caccggtgat gaaggcatcg aggaacatgt
     1801 tccgcatcga aaggaaatcc tcggcggtat ggtgcaggcc gaagacgaga tcgagggaga
     1861 catcggacct cggttcgaga tgccacccga catcggcata ggaattgagc cgcgatcgct
     1921 ccggcacatc gagccccgtt tctgccgcaa gcgagaccga ggcggaaaag gcatcgtcgc
     1981 gcccgaaggc gacaccgccg agcgcggcat tgcgcagaaa aacgcctcgt cgctccccca
     2041 tcgtcaggag atcgactatc gatagatgct ctatattact gtcgaaaaga gtcccgatgg
     2101 tgccctccgc ccagaagcgc tcggcaaagg agacgacggg gtaaagaaag acgaaaagaa
     2161 gcgcaagaac actattcgat acggcggttg cgatagttcc ggtctttccg ttcttctcga
     2221 agcgtgtcac gccgttcctt ctttcgtttt tctatgctat cgtcccccgt ttcctcttct
     2281 ttgtcaattt ttgagacggg ggggacggtc gtttgccttg ttcgttcgtg atgcgccggc
     2341 gccacggctc ccttgacgat cttttccacg gcacgggaac ggggggcatc ttcctgacgt
     2401 ggcgatggcg agggctccgc ctcggggtcc ggtcgtataa gatcggcgac ggggcgacga
     2461 tcgttcttga ccggcgtgac ggcccgggtt ttcctaggga gcggcggtgt agtattcagg
     2521 atatcggcat cagcactttc ccgtggcgtg gtgagggtcg cggaaggttc ggccggagga
     2581 aggaaccgtt cttcccagtc gggcgcgacg accccctcaa ggatcctgac ggtacttttg
     2641 agttccggta ccgccttgga aagacagatg aggggaagga acagaagaag gcatgcgcta
     2701 gttaagcgac ccatgcacca cctcggagcg gagccggtac tgggcgatca ccaccgtcaa
     2761 gccgtcggcg gcaagcacga cacggtgccg atgcaccgcc ccttcatagg aggcgcttgt
     2821 ccgtatctcc ctgacgccga cgcgatcgac cgtcacgacg aacggtatcg tgttgctccc
     2881 cttgacgagc ggaccggtcc acgcaagcat gcggcgggag gatatctcgg ggacggagct
     2941 gtggaaggaa agtccctcgt caagatcgat ggtgaccgtc acctgttcga tatcctgcga
     3001 cgcctcatag gcgagttcta tggttatcgg tcgcccggcc gagacctcgg cgcgcgagat
     3061 aaggacccct tcctcctccg acgacggtcc ctgccgaacc gcaatgagaa cgaccgccag
     3121 cgccaccacg atgcccagcg ccgcaacgcc ccagcgcaat ccgccccaga gtgaacgccc
     3181 tttcccttcg cgcgaacggc cctgttgcct ctcgaccaat ttgatgtgaa gcgtggcatc
     3241 gaaatcctcg gggatggcaa cgggacgggc cttcaggtca gccatcccct gcgccatctt
     3301 ctgcgcgatc gcgaattcct cggcgcacga gcgacagcgg gcgcagtgtt cccgcaggtc
     3361 ctgttcggcg gcgggatcga gggcacgacc cagccagccc accaggagtt cacgatattt
     3421 agcacacgcc gacatcagcg aacgctcctc tcagtacgga taatagaccg accattcatg
     3481 aaaaaagttc cctgttttcc aacaccaacg cgaaaagccg ctcgcgcgcg cggaagagcc
     3541 gcgacttgag cgccccttcc gatatcccca cgatacgggc cgcttcgtca taccggagtt
     3601 cctgcagatc gaccagttcc aacacctcgc gatgttcggg agaaagcgat ccgataaggc
     3661 gacgtaggtg ctcgccccgg tcccgttggt gaagcgaatc gcgaaaatcg ctctcctgcg
     3721 ccaattcctt ttcgggcagg tcgtcgaccg aaagattttt cgatgtcttg gggctcgcac
     3781 agtaatcgat gaactcgtta cgagcgatgc gccacagcca ggtcatgaat gacgcctcgc
     3841 cccggaatct cgatatgttc aagaacgctt tgaggaacgc ctcctgcagg atgtcggcgg
     3901 cggcggcatg atcgccgccc gtcatgccga aaagataggc gtacagcccc tgtttgtact
     3961 ttccgatgag ttcctcgaag gcggcgacct cgccccgtat cgagcgacgc acgagttccg
     4021 cgtatcgttc ctgcttcatg gaagcgttgt acccccatcc gatccttttt tcaactattc
     4081 cggtgaacgg acagaaaatt tgcaaacgga ccggcggacg ctagaatgct cagtcaccga
     4141 acgcacggga gcgcatccat gaacctcacc gaaacactcc ttcttgcgac cagcctttcg
     4201 atggacgcct tcgcggtcgc catcgccgcc ggcgtcggct caccgcgcgc cgaccgacgt
     4261 gtcgccggca ccatcgccct gtttttcggc ggctttcagt gcctcatgcc ggtcatcggc
     4321 ttttttgtcg ccggggcttt ctacggatac atctgccatt ttgaccactg gatcgcctgc
     4381 gggctgctcg ttctcatcgg cggtaggatg atctacgaag cggcgaccgg cgacggctgt
     4441 ccgcgctcgg tcgatctcgg accccggtcg cttctcgttc tcgcgctcgc cacctcgata
     4501 gacgcgctcg ccgccggtgt cgggctcacc ttcctctgct cctcgatatg gctccccgcc
     4561 ctgaccatcg gtgtcgtcac tttcgccttt tccttcgttg gtttcctgtt cgggcgacga
     4621 cttggctgcg cgttcggcag aaagatggag atactgggcg ggctgacctt gtgcctgttg
     4681 ggactaaaga tactggggga agggcttatc gacggttagc cgttacttgc cgcacccgcc
     4741 gcctttcggc atggcgccgg gtgcggcaag taacggctaa ccgccgataa gcccttcccc
     4801 c
//
LOCUS       DAOQUE010000196         4785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_19762_length_4785_cov_3.693658, whole genome shotgun sequence.
ACCESSION   DAOQUE010000196 DAOQUE010000000
VERSION     DAOQUE010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19762_length_4785_cov_3.693658"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..927)
                     /locus_tag="PKH10_07035"
     CDS             complement(64..927)
                     /locus_tag="PKH10_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004349284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNT27915.1"
                     /translation="MSKIVVLAKQVPDTSNIRGNAMKEDGTVNRAALPTVFNPEDLNA
                     LETALQIKDRTGREVVLLTMGPLKAVEIIKQALYMGADAGYLLTDKKFAGSDTWATSY
                     ILAEAIKKIGGVDLVVAGRQAIDGDTAQVGPQVAEKLGFDQVTYVTALNEVTDTTVTV
                     ERDGEFQTEVIRARSPLLLTVTSAANTPRYPSARQMLRYFKADIKENIPAEEQAEYER
                     NGWIIPTLTFADIPGINEAHIGLKGSPTKVNAIRDIAVQGGDLKMYDPRLDGVKALVR
                     DLMKQYVEVQS"
     gene            1274..1867
                     /locus_tag="PKH10_07040"
     CDS             1274..1867
                     /locus_tag="PKH10_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT27916.1"
                     /translation="MHAMTGRETRNSEEKGLAARAAGGDIRAFEELIMAERPRLYSFA
                     LAMSGGNHADAADVLQEAFIKAFVNIRRFRGDSSFGTWLWRIVHNEFLNYRVALRSRP
                     IRQEGDDETVSRLPSDHPGPAESLIAAERRAKVLELVALLPPKYREAIVMIDIREQPY
                     DETAELLGISMSALKTRLMRAREKFSELVRKRKEHFI"
     gene            1880..2602
                     /locus_tag="PKH10_07045"
     CDS             1880..2602
                     /locus_tag="PKH10_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27917.1"
                     /translation="MKPCERTIQLVAASMGRDLERDEALFLKEHAAVCPECRAHLAAS
                     DTAIGILKGGLVPQEIDTPPLFNERLRERLRERAGHPSWYRYLPQIRFRRLELGFLAA
                     LLLLAVGAVTFFYFHEDAPQPQLVTQKEVSTDTPLSIDLEYLAVHPIAEVTVTIELDE
                     GVVFHSDYREIADARSHTWKGRFNEGINSIPFMVTVEKPGIREIRTRADIGDWRHEHR
                     ITLTSNGKMLTMAMYRLPKRRI"
     gene            2607..2972
                     /locus_tag="PKH10_07050"
     CDS             2607..2972
                     /locus_tag="PKH10_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27918.1"
                     /translation="MRSVLLLLTLLPATALLWADGSGVTSRVRISSNETNRGWEQTFF
                     DAVALAGMTRLSDEPADALPMPDFDTPDTAGQDRAKKVSAPPPRRTVEYHIIQEESEQ
                     GDRSRKVKKRVVIEPPTKP"
     gene            2988..3773
                     /locus_tag="PKH10_07055"
     CDS             2988..3773
                     /locus_tag="PKH10_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNT27919.1"
                     /translation="MNTPAATGFDIGSRTGKAVVIDSAGALLHTATVETVGNAIRTFD
                     DLRALLPPDLPDDLPSAATGYGRAAIEGRVRRTATEISCHAIGALRRLPGAATVIDIG
                     GQDAKVIILEEGRIRDFAMNDKCAAGAGRFLEVMVPRLGLTLESFSRLDVAHIEPLPL
                     SATCTVFAESEIVSLMASGADPVVLASSVAAMAARMAALLAAPLHARPPFFMTGGVSR
                     IVPVRHHLSLILGHPVETSDDASLSGAHGAAIMALRDGALPFR"
     gene            3838..4782
                     /locus_tag="PKH10_07060"
     CDS             3838..4782
                     /locus_tag="PKH10_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNT27920.1"
                     /translation="MKKVSLLIICSVLLCFSCEENKKLPLNDDIAVDDGDTIVYDNDQ
                     KESELPDVTDNAPVDDAVSPTDEDGLLSDEDLITKTTCLETGEGCAGNEICLYDWQLA
                     GYYCEDACDPLVTDDCPEGLWCEPVAGDTKSGCFLPVIIAGRVFDLDEIGYPAIEGAR
                     VSAMDQKDGSATDVSVTDSTGHYAIPLSLIRQRNGMPLADEVYIMRAAAKDHAPYPGP
                     GQVALPILMENCTLMGDGYYVSSGFLDIGLAALAEEAQGGFIVSGSLSETTSGVLVIA
                     RCATAPCPYTYTGAEGDFTIYNVLPGDYEIVKSPSAPV"
BASE COUNT          895 a         1549 c         1408 g          933 t
ORIGIN      
        1 gttccagcgt cacattgcgg agccgttcgg tgccggcccg cttttctatg aataccgccg
       61 ttttcatgac tgcacctcga catactgctt catcagatcg cggacgagcg ccttgacgcc
      121 gtccaatcgc gggtcgtaca tcttgagatc tcccccctgc accgcgatat cgcgtatcgc
      181 gttcaccttg gtcggacttc ccttgaggcc gatatgcgcc tcattgatgc cggggatgtc
      241 ggcaaaggtg agcgtcggga tgatccagcc gttccgttcg tattcggcct gttcttcagc
      301 cgggatgttc tctttgatgt cggccttgaa gtagcggagc atctggcgcg ccgaagggta
      361 gcgcggggta ttggcggccg aagtgacggt gaggaggagc ggggatcgcg ccctgatgac
      421 ctcggtctgg aattcgccgt cccgttcgac ggtgacggtc gtgtcggtca cctcgttcag
      481 cgcggtcaca taggtcacct gatcgaagcc gagtttctcg gccacctgcg gccccacctg
      541 cgccgtgtcg ccgtctatcg cctgccggcc agcgacgacc agatcgacgc ccccgatctt
      601 ctttatcgcc tcggcgagga tataactcgt cgcccaggta tcggacccgg cgaatttctt
      661 gtcggtcagc agatacccgg cgtcggcacc catgtagagc gcctgcttga tgatctcgac
      721 cgccttgagg ggccccatcg tgaggagcac gacctcgcga ccggtcctat ccttgatctg
      781 gagcgccgtt tcgagcgcgt tcaggtcctc gggattgaag acggtgggca gggcggcccg
      841 gttgacggtg ccgtcctcct tcatcgcgtt gccccggata ttcgaggtgt ccgggacctg
      901 cttcgccaag actacgattt tactcataat tcctccatga ctgtcataaa aaaatgacac
      961 ttcggtctag ttcctaacaa ataccaccgc cgatgtcaac cattttttct gtgacccccg
     1021 gcaggcggcg cctgtcataa gggcgagagg atgttacgga gggaccgccc gtgaaggaca
     1081 tcgttcgtgt agtgcccgcg atggtactct tcatgatctt catcgccgtt tatctggtgg
     1141 ccgaacactt cctcggcgca ccactcccct agcccccgct ccaccgtgac gggatatttt
     1201 tcaccgaaag ttgcagtgtc ctttcttttt cgtaacatcg gaacagcggc aactgtcaaa
     1261 accgatagag gatatgcatg cgatgacggg gcgcgagacc cggaacagcg aagagaaggg
     1321 actggccgca cgggcggccg gcggcgatat tcgggctttc gaggaactta tcatggctga
     1381 acggccgcgc ctttattcgt tcgcactcgc catgagcggc ggcaaccacg ccgatgccgc
     1441 cgatgtcctg caggaggcct tcatcaaggc cttcgtgaac atccgccggt tccgcggcga
     1501 cagttcgttc ggcacctggc tgtggcgcat cgtccacaac gaattcctga attaccgcgt
     1561 cgcgctccga tcgcgaccga tccggcagga aggcgacgac gagacggtct cccgcctgcc
     1621 gagcgaccat cccggcccgg ccgaatcgct catagccgcc gaacggcgcg ccaaggtgct
     1681 cgaactggtc gcgctcctgc ctcccaaata ccgcgaggcg atcgtcatga tagacatccg
     1741 ggaacagccg tacgatgaga cggcggaact gctcggcata tcgatgagcg ccctcaagac
     1801 ccggctgatg cgggcccgcg agaagttctc ggaactggtg cgaaaaagaa aagaacactt
     1861 tatatgatgc gaggtcgcca tgaagccatg tgaaaggacc attcaactcg tcgccgcgtc
     1921 catggggcgc gatctggagc gtgacgaggc gctctttctc aaggagcacg ccgctgtctg
     1981 tcccgaatgc cgcgcacatc tcgcggcatc ggacaccgcg atcggcatcc tgaagggcgg
     2041 actggtcccg caggagatcg acacaccgcc gcttttcaat gaacggctcc gcgaacggct
     2101 ccgcgagcgg gcggggcacc cctcgtggta ccgctacctg ccgcagatac ggttccgccg
     2161 tctcgaactg ggctttctcg cggcgcttct cctgctcgcg gtcggcgcgg tgaccttttt
     2221 ctatttccac gaggacgcgc cgcagccgca actggtgacc cagaaagagg tctcgaccga
     2281 cacgccgctt tcaatagacc ttgaatacct tgccgtccac cccatcgcgg aggtcacggt
     2341 gaccattgaa ctcgacgagg gggtggtctt tcacagcgac taccgcgaga tagccgacgc
     2401 ccgttcccat acatggaaag gtcgtttcaa cgagggaatc aattccatac cctttatggt
     2461 gacggtcgag aagccgggga tccgcgagat acggacccgc gccgatatcg gggactggcg
     2521 tcacgagcac cgcatcaccc tcacctcgaa cgggaagatg ctcaccatgg ccatgtaccg
     2581 gcttcccaag cggaggatct gacccgatgc gctccgttct tcttcttctc accctgcttc
     2641 cggccaccgc cctcctgtgg gctgacggat cgggagtgac cagccgcgtc cgcatttcca
     2701 gcaacgaaac gaacaggggc tgggaacaga cctttttcga cgccgtcgcc ctcgccggca
     2761 tgaccagact ttcggatgag ccggccgacg cgctcccgat gcccgatttc gatacgcccg
     2821 ataccgccgg acaagaccgg gcgaaaaagg tgtccgcgcc gccgccccgc cggacggtcg
     2881 aataccacat cattcaggaa gaatctgagc agggcgaccg gtcacgcaag gtgaagaaac
     2941 gggtcgtcat cgagccgccc acaaaaccgt gagaccagcg taaccccatg aacacccccg
     3001 ccgcaacagg ttttgacatc ggttcgcgca ccggcaaagc ggtggtgata gacagcgccg
     3061 gtgccctgct tcacactgcg acggtcgaaa cggtcggtaa cgcgatccgg accttcgatg
     3121 atctacgcgc cctcctaccg cccgacctgc ccgacgatct tccctccgcg gcgaccggct
     3181 acggacgggc cgcgatcgaa gggcgggtgc gtcgtaccgc gaccgagata tcctgccacg
     3241 cgatcggcgc cctgcgtcga ctccccggcg ccgccaccgt catagacatc ggcggacagg
     3301 acgccaaggt catcatcctt gaggaaggcc ggatccgcga tttcgcgatg aacgacaaat
     3361 gcgccgccgg cgcgggaaga ttcctcgaag tgatggtgcc ccgtctcgga ctcacactgg
     3421 agagtttttc ccgactcgat gtcgcgcata tcgagccgct tcccctctcg gcgacctgca
     3481 cggtattcgc cgaatcggag atcgtgtcgc tcatggcgtc gggcgccgat cccgtcgtgc
     3541 ttgcatcatc ggtcgccgct atggcggcac gcatggcggc cctgctcgcc gcccctctgc
     3601 acgcccgccc gcccttcttc atgaccggcg gcgtctcccg catcgttccg gtccgccacc
     3661 acctgtcgct tatactgggt catccggtcg agacctccga cgacgcatcg ctttcggggg
     3721 cgcacggcgc ggcgatcatg gcgcttcgtg atggcgctct cccttttagg tagacttctt
     3781 cccgtcagtg tgctagtatc tctccgttcc ggatcattct tgctcaggag gggtcccatg
     3841 aagaaagtat cgcttcttat catctgttcg gttcttctgt gtttttcctg cgaagagaac
     3901 aaaaaactac cgctgaacga cgatatcgcg gtggacgacg gcgacaccat cgtctacgac
     3961 aacgaccaaa aggagagtga actccccgat gtcaccgata acgctcccgt tgacgacgcg
     4021 gtctccccga ccgatgagga cggcctgctc tccgatgagg acctcatcac caagaccacc
     4081 tgtctcgaaa cgggtgaagg atgcgccggg aacgagatat gcctttacga ctggcaattg
     4141 gcaggatact actgcgagga cgcctgcgat cctctagtga ccgacgattg ccccgaagga
     4201 ctgtggtgcg aaccggtcgc gggcgacacg aagagcggct gtttccttcc cgtcatcatc
     4261 gcggggcggg tgttcgacct cgatgagatc ggttacccgg ccatcgaggg agcgcgcgtc
     4321 agcgcgatgg accagaagga cggctcggcg accgatgtgt cggtgaccga tagcaccggc
     4381 cactacgcga taccgctctc gctcattcgg caacgcaacg ggatgccgct agcggacgag
     4441 gtctatatca tgcgggccgc cgccaaggac cacgccccct atcccggacc ggggcaggtc
     4501 gccctgccga tactcatgga gaactgcacg ctcatgggcg acggttacta tgtatcgagc
     4561 ggtttcctcg acatcgggct cgccgctctc gccgaggagg cgcaaggcgg gttcatcgtg
     4621 tcgggatctc tttccgagac gacgagtggt gtgctggtca tcgcccgttg cgcgaccgca
     4681 ccgtgccctt acacctacac cggcgccgag ggcgatttca ctatctacaa tgtcctgccg
     4741 ggcgactacg agatagtgaa atcgccctcg gcgccggtgt aggtg
//
LOCUS       DAOQUE010000197         4746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_19966_length_4746_cov_4.028565, whole genome shotgun sequence.
ACCESSION   DAOQUE010000197 DAOQUE010000000
VERSION     DAOQUE010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19966_length_4746_cov_4.028565"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PKH10_07065"
     CDS             <1..826
                     /locus_tag="PKH10_07065"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT27921.1"
                     /translation="WHNFLFNQMLSQAFDNYVSPEILGWLRETGGDALRSDSAERRDI
                     TILFSDVAGYTTLSNALDADTIMRSLRFYLDRMLAIIQRHNGYVDKINGDGLMVLFGA
                     PKHSDHHAEEAVTTAREMQEEVARMQEEWRRFTDRPLRIRVGVASGRVFVGNLGGTGH
                     IGYTAIGQDVNLAARLESASEIGGILISPECYAKLERKPIAWPRTVHLKGYADDLAVH
                     QIPPLAALVPDPTGAGRILLSRESYEELKEKPAGAFMEIPGETGPRQVFRVDTAQG"
     gene            1083..2339
                     /locus_tag="PKH10_07070"
     CDS             1083..2339
                     /locus_tag="PKH10_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27922.1"
                     /translation="MRNILILFTALVIGFSLAAEEKKPKIAVMEIEDKSGRIDKEFLE
                     NAAEALRAELIATDKFIVISKDRQRKAMIKGAKKESWKECYDQSCRIELGQALTADNI
                     LSGIITYFGKKYTLSIEMIDLAKEATIKGASADFDATEEVSIAKAVRETIAVVVGVAS
                     KRINAVQSVDGGQGESKYASMVNKEKREKEKLDAAWAEVRDIALDRNIPKEKRVTVLN
                     EFLNDFPKNNDHEEKAEELIDILDDDDEPEMVWAQKEADEKRKGTYEYSLLEGTEWFA
                     FRIEAGSHGGGGMLSFFTLRWKWFYWEILRGGGAYGGPGLLGSSAFYGLAGGSFGVPI
                     RFSNVLEHEMRLGVGFYGGAMNIETGEGYSSYSYEGLASMGLEIGYVWHISKCCSLHG
                     GLETYLSLWPHWDPTAIMVFVGMRFR"
     gene            2430..2915
                     /locus_tag="PKH10_07075"
     CDS             2430..2915
                     /locus_tag="PKH10_07075"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNT27923.1"
                     /translation="MVDVRSIPDIHFFCAGASEGKTELTSFDGALIKAGIGNTNLIKM
                     SSILPPRSTEVKAVAIPPGSFLPLAYAAITSTEPGETISAAVAIAIPDDRSLNGVIME
                     HHNHAPLEYVEAKVREMAEESMEMRGIKKFTVRSKGVEAKVVHAATAFAAVCLWYKDL
                     M"
     gene            complement(2907..>4746)
                     /locus_tag="PKH10_07080"
     CDS             complement(2907..>4746)
                     /locus_tag="PKH10_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNT27924.1"
                     /translation="AIALRGLTIAEFPDGAVGIRRMLAEILAAKRGERTRESMANYSE
                     TLLAERFFFMRLASGSVGGKGRGLSFIEHVLHRAGLDAAVPGLNIRLPRTAIIGTDEF
                     HLFVDGIGQQRILQMNDEGAVRSLFLRTPLTLTLRQRLRVLLGKVTGPLAVRSSGLFE
                     DSLSQPFAGIYETYFLPNSHPDPEVRLRQLEEAIRLVFASVYSPLARSYFDSVQYEME
                     EEKMAVIIQELVGRRRGDRFYPHISGVAQSYNYYPFAKMKPEDGIALLAVGLGKAVVD
                     GEQVFRFCPRYPKARNQSLKDMLNASQRYFYALDMRKSDADLLEGEMATLVRPEVIEA
                     EQDGMLDMCVSTYDGAQNLKKPGLHAAGPRIVDFSYILEYKQVPLAGAIQTTLDIMKH
                     ALGTPVEIEFAVDLDEADNGAPTLYLLQVKPLVRNTADHHIDPATLDRSNLFLYTTRG
                     MGNGIVEDVRDIVYISPDRFDRLKTEAMAVEIAAINKVLKAENRRYILIGPGRWGSSD
                     RFLGVPVDWSAISQTRVIVEAGLSDFSIDASLGSHFFHNITSMNIGYFTVAAHSEEDL
                     IDWKWLAAQPLVAEKQYARHVRLERPVLVRMDGRSGIAAIGKPPLH"
BASE COUNT         1025 a         1322 c         1478 g          921 t
ORIGIN      
        1 gtggcataac ttcctgttca accagatgct ctctcaggcc ttcgacaact atgtgtcacc
       61 cgagatactc ggctggcttc gcgaaacggg cggcgacgcg ctccggtccg attcggccga
      121 acggcgtgac atcacgatac tcttctccga tgtcgccggc tacacgacgc tctccaacgc
      181 gctcgacgcc gacaccatca tgcgctcact ccggttctac ctcgatcgca tgctggccat
      241 catccagcgg cacaacgggt atgtggacaa gataaacggc gacgggctca tggtgctgtt
      301 cggcgcgccg aaacactccg atcaccacgc cgaggaggcg gtcaccacgg cgcgggagat
      361 gcaggaggag gtggcgcgga tgcaggagga gtggcgtcgc ttcaccgacc ggcccctgcg
      421 gatacgggtc ggcgtcgcga gcgggcgggt gttcgtcggt aacctcggcg gcaccgggca
      481 tatcggatac accgccatcg gacaggatgt caacctcgcg gcgcgactcg agagcgcctc
      541 ggagataggc ggcatcctca tatcgcccga atgctacgcc aaactggagc ggaaaccgat
      601 cgcatggccg cgcacggtgc atctcaaagg gtatgccgac gacctcgcgg tccatcagat
      661 accgccgctc gccgcactcg tccccgaccc gaccggcgcc gggcgcatcc tgctgtcgcg
      721 cgaaagttac gaggaactca aggagaaacc ggcgggcgcc tttatggaga taccgggcga
      781 gacgggtcct cgacaggtct tcagggtcga taccgcccaa ggttgacttt tcaaaaacac
      841 ccaccggccg cgtggcgcgc cacctctcat tacttcctcg ccctgtgaag ggagaggatc
      901 gaggcttgcc ggggcgtagc cacaggcgaa gaccggtgag ggtgttcttt gtaaaccctc
      961 atccgccgct atcgcggcac cttctccctc tacaggtaga aggtatatgt aaggtacagc
     1021 gtttttcttg aactggcggt cggtttcggt tacactgcag catcttcgac ggggaggctt
     1081 tcatgcggaa catcttgatt ctttttacgg cgttggtcat cggtttctcg ctggcggccg
     1141 aagagaagaa accaaaaatc gccgtcatgg agatagaaga caagtccggc aggatcgata
     1201 aagaattcct tgagaacgcc gccgaggcgc ttcgcgcgga actcatcgcg acagacaagt
     1261 tcatcgtcat ctcaaaggac cgtcagcgaa aggcgatgat aaaaggagca aaaaaggaaa
     1321 gttggaaaga gtgttacgat caatcgtgcc ggattgaact cggtcaggca ctgaccgcag
     1381 acaatatact gagcggaatc atcacttatt tcggtaagaa gtacactctc tcaatagaga
     1441 tgatagacct cgcaaaggag gcgaccatca agggggcttc ggccgatttt gacgccaccg
     1501 aagaggtgag catagcaaaa gcggtcaggg agaccatcgc cgtcgtggtt ggggtagcgt
     1561 ccaagcggat aaatgcggtg caaagtgttg acggcggtca gggagagagc aagtacgctt
     1621 ccatggtgaa caaggaaaaa agagaaaagg aaaaactcga cgcggcatgg gctgaggtcc
     1681 gcgacatagc ccttgacaga aacataccta aagaaaagcg ggtaacggta cttaatgagt
     1741 tcctgaacga ttttcccaag aacaacgatc acgaggaaaa agcggaagaa ctgatagaca
     1801 tccttgatga tgatgacgaa ccggaaatgg tgtgggccca gaaagaggcg gatgagaaaa
     1861 gaaagggaac ctatgaatac tctctgctgg aagggactga atggttcgcg ttcagaatag
     1921 aagcaggcag tcatggtggt ggcgggatgc tttcattctt tactctgcga tggaaatggt
     1981 tttattggga gatactgaga ggagggggag catacggggg cccgggtttg ctggggagtt
     2041 ctgcttttta cgggttggct ggtgggtctt ttggcgtacc aatacgattc tcaaatgttc
     2101 tagagcatga gatgagattg ggagtcggtt tttacggtgg agcgatgaac attgagacag
     2161 gcgaaggtta ttcttcttat agttacgaag ggctcgcctc tatgggccta gaaatcggtt
     2221 atgtttggca cattagcaaa tgttgctctc tgcatggagg gctagaaaca tacctttctt
     2281 tgtggcctca ctgggatcct accgctatca tggtttttgt cggtatgagg ttccggtaaa
     2341 cgacttttca tttgtaaatc acaaaacttt tgactctctc ccgttcgtgg ataccatcgg
     2401 tgcgacaacc tttctgacgg aggatgacga tggttgatgt gcggagcatt cccgacatac
     2461 acttcttctg tgccggagcg agcgaaggaa agacggaact gacctcgttc gacggcgcgc
     2521 tcatcaaggc gggcatcggc aacaccaacc tgataaagat gagcagcatc ctgccaccgc
     2581 gctccaccga agtgaaagcg gtcgcgatac cgcccggcag tttcctgccg ctcgcctacg
     2641 ccgccatcac ctcgaccgaa ccgggtgaga cgatatcggc cgccgtcgcg atcgcgatac
     2701 cggatgaccg gtcgctcaac ggcgtcatca tggagcacca caaccacgcc ccgctcgaat
     2761 atgtcgaggc caaggtccgc gagatggccg aggaatcgat ggaaatgcgc ggcatcaaga
     2821 aattcaccgt ccgcagcaag ggtgtcgagg cgaaagtggt ccacgccgcc accgccttcg
     2881 ccgccgtgtg tctgtggtat aaggatctaa tgtagcggcg gtttccctat cgccgcgatg
     2941 cccgaccgac cgtccatcct gaccagcacc ggtctttcca gccgcacatg ccgcgcgtac
     3001 tgtttctcgg cgacgagcgg ttgcgccgca agccatttcc agtcgataag gtcctcttcg
     3061 gaatgggccg ccacggtaaa gtagccgatg ttcatcgagg tgatgttgtg gaagaagtgc
     3121 gatccgagcg aggcgtctat cgagaaatcg ctgagccccg cctcgacgat gacgcgggtc
     3181 tgcgatatcg ccgaccagtc gaccggcacg ccgaggaagc ggtccgacga accccagcgc
     3241 cccgggccga tgaggatgta acgccgattc tcggccttga gcaccttgtt gatggctgcg
     3301 atctcgaccg ccatcgcctc ggtcttcaag cggtcgaaac ggtcggggct gatgtagact
     3361 atgtcgcgca catcctcgac gatgccgttc cccatccccc gcgtcgtgta gagaaagagg
     3421 ttcgaccggt cgagcgtcgc cggatcgatg tggtggtcgg cggtgttgcg gacgagcggt
     3481 ttgacctgca ggagatagag cgtcggcgcg ccgttgtcgg cctcgtcgag atcgacggcg
     3541 aactctatct cgaccggcgt accgagcgca tgtttcatga tgtcgagcgt cgtctgtatc
     3601 gcaccggcga gcgggacctg cttgtattcg aggatgtagg agaaatcgac gatgcgcggc
     3661 ccggcggcgt gaagccccgg ctttttgagg ttctgagcgc cgtcgtaggt cgatacgcac
     3721 atatcgagca tgccatcctg ctccgcctcg atcacctcgg gacgaacgag cgtggccatc
     3781 tcgccttcga ggagatcggc gtcgcttttg cgcatgtcga gcgcgtagaa atagcgctgt
     3841 gaggcgttca gcatatcttt gagcgactgg ttgcgcgcct tggggtaccg cgggcagaaa
     3901 cggaacacct gctcgccgtc gaccaccgcc ttgccaagac ccaccgcgag gagcgcgatg
     3961 ccgtcctccg gcttcatctt ggcgaaggga tagtagttgt aggattgcgc gacgcccgag
     4021 atgtgtgggt agaagcggtc gccgcgccgc cggccgacca gttcctggat gatgaccgcc
     4081 atcttctcct cttccatctc gtactgcacc gaatcgaagt aggagcgggc gagcggcgaa
     4141 tagaccgacg cgaacacgag tcgtatggcc tcctcgagtt ggcgcaggcg cacctcggga
     4201 tcggggtggc tgttggggag gaaataggtc tcgtagatgc cggcgaacgg ttgcgagagc
     4261 gagtcctcga acagacccga cgacctgacc gcgagcgggc cggtgacctt gccgaggagg
     4321 accctcaggc gctggcgaag ggtgagcgtc aacggagtgc gcaggaacag cgaccggacc
     4381 gccccttcgt cgttcatctg caggatgcgt tgctggccga taccgtccac gaagaggtgg
     4441 aactcgtcgg tgccgatgat cgccgtgcgc ggcaggcgga tattgagccc ggggaccgcc
     4501 gcgtcgagcc cggcgcgatg gagcacatgt tcgatgaacg aaaggccgcg ccccttgcca
     4561 cccaccgacc cgctcgcgag ccgcatgaag aagaaccgtt cggcaagcaa cgtttctgaa
     4621 taattcgcca tcgattcgcg ggtccgttcc ccgcgcttgg cggcgagtat ctcggcgagc
     4681 atgcggcgga taccgacggc gccgtcgggg aattccgcta tcgtcaggcc gcgcagggcg
     4741 atggcg
//
LOCUS       DAOQUE010000198         4738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_20022_length_4738_cov_4.142430, whole genome shotgun sequence.
ACCESSION   DAOQUE010000198 DAOQUE010000000
VERSION     DAOQUE010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20022_length_4738_cov_4.142430"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2695)
                     /locus_tag="PKH10_07085"
     CDS             complement(<1..2695)
                     /locus_tag="PKH10_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNT27925.1"
                     /translation="MNGSLLMTASAGAGKTYQLTKVVRGLIAGDPALIVAVTFTRAAA
                     AEMEKRILDRIAGEDTPPVERLRLLMRAAQVRYSTIDALFHQFLATEDRAPQMADDHE
                     KDLIKALADERFFTRPEVIDATEEILIAARILKITPERLTCELDNDRDALAAWNCPIG
                     LLDELKAKQARIYAAYEKLRKEVTAVGAVTAGNLLKQVVIPLSRSLDKIDLSKALFLK
                     HDIAGVKVSAADQKLAAYTKLRALYPPMRRLVAEYVVNTARLRTALLKRFSALRAESV
                     ETEKRRLDRRYFEDVLRELIALDGSDSPDRPELSARLYERGYHRTAHLLLDEFQDTSA
                     IQMELLRPLLEDIAGDIGENGAGERSLFFVGDWKQSIYRWRGADPERLQRWLDPLRRS
                     GQLPTEKLLYNWRSTPLLIGFFNELTKELFAGNESAGDTQEAPPEEERRDPYTGLSSV
                     AVIPVSCGSGDGELYERLVAEIRRRREETGCAWGDITVLCRSHAHLAKVAKGLAAAGE
                     GEEPVGTSDLTGRELLSLREGTALYLALAAIFSGGEEFVERALPELGQSDLTDTVHRL
                     IADAARWPAPHRFSALAAALRELAPRFPRTIVETLWDEAERYFDRPDTGDATDFLRYL
                     LAVGNLVTVPEGEHSDRVKLATMHSAKGLEFPHVFVLWKDTNDRNGPVTDPDDGCLLD
                     LNKVALEFLATGPIPGATRIAGTAEEHRDMNAAETANLLYVAATRAKRSLTIVVRANK
                     EGGLTGFGEKMRRAAQQPLPGFERTDLGHRHDYGKAGPMDADHEDLSLVTLGPVATMP
                     DEDDIDPALRSADIEAGIARGLRIHAALGGLSPRLDMPKRADLTPEERKVLEKFLRDP
                     AVREILSRPGTVLCEQHLSDRNTFGVVDRLIIEIRRLKNRQY"
     gene            complement(2692..>4738)
                     /locus_tag="PKH10_07090"
     CDS             complement(2692..>4738)
                     /locus_tag="PKH10_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNT27926.1"
                     /translation="VTGDELARLDRIAPEHMERIAIFFATMGTFRRELATEGLFYAPF
                     EEAAFADLTLRDNELFVGLPVMTPTHERFISRIPHDRRFIDAPLFGPHMPQGTPDYDS
                     ALALIRRLGIGEERATTGRLELTELNERPAVRALIAAEIERFLQGGEGGNKGQLLIVP
                     LDETLSFYLWQLLFRPLGDRVNFVPWLPFSHFVAAHRLIMAIRERRPLDGVRRELAAE
                     LTARWRDLDAAERAAFEKAIALIDDMTRLGPLMGAERETLSLHLVASAKLHLRGDRKA
                     PVQVVGLGNATGVPYRRALILPMDRDIFPRRPFNGPFLNLVHLPRIHKTQFESDDLTL
                     RQFLALGESAHLAARYDTGAGAAPSPHFAFLSVEFGMKPVKRLMAAAPFIAPSGDPAI
                     KNSDEMRERLANYPWSFSSLPLFLSCPFRFVIERKDFFNIEPPACFEGEDHVNMVIGQ
                     FLHDLFASLKGVDKPIDRWRQLFEERWESNAEISTKVPDRAVRKALVHAYLNDIAAWE
                     RESGAAVLFSNAVTDTELPLEATFGGYRLTGRLDRVQERNGRKLVADLKYKESMPAVC
                     KEGLIAEAADERGLHDHFQLLFYAQLLIENGRAKEDELDAAYICLRSGDRGNYIVELP
                     AGEIERRRETLDALARRLDRTIAQERFAPNHKARACSYCPYKALCLRPDLYFVGRPE"
BASE COUNT          791 a         1506 c         1532 g          909 t
ORIGIN      
        1 cgtactgccg gtttttcaaa cgccttatct cgatgatgag tcgatcgact acgccgaagg
       61 tgttccggtc cgacaggtgc tgttcgcaga gcaccgtgcc gggacgagaa agtatctcgc
      121 gcaccgccgg gtcgcgcagg aatttttcga gcactttacg ctcttcgggc gtcaggtcgg
      181 cacgcttggg catatcgagt ctcggtgaaa gtccgccaag cgccgcatgt atccgcagac
      241 cgcgcgcaat gcccgcctcg atgtcggccg accggagcgc cgggtcgata tcatcctcat
      301 cgggcatcgt ggcaacgggg ccaagtgtca ccagcgacag atcctcgtgg tcggcatcca
      361 tcggtccggc tttcccgtaa tcgtgccggt ggccaaggtc ggtcctttcg aaccccggca
      421 gcggctgttg cgcggcgcgg cgcatctttt cgccgaaacc ggtcagcccc ccctccttgt
      481 tcgcgcgcac gacgatggtg agcgatcgtt tggcccgcgt cgcggccaca tagagcaggt
      541 tggccgtctc ggcggcgttc atgtcgcggt gctcttcggc cgttccggcg atacgggtcg
      601 cccccggtat cggcccggtc gcaaggaatt cgagcgccac cttgttcagg tcgagcagac
      661 agccgtcatc cgggtccgtc accggcccgt tgcggtcgtt cgtatccttc cacagcacga
      721 agacatgtgg gaattcgagc cccttggcgc tgtgcatcgt cgccagtttc acccggtcgg
      781 agtgctcccc ctcgggcacg gtgacgaggt tccccaccgc gaggagatac cgcaggaaat
      841 ccgtcgcatc gccggtatcg ggacggtcga agtagcgttc cgcctcgtcc cagagcgtct
      901 cgactatggt gcgcggaaag cgcggggcga gttcgcgcag tgccgcggca agcgccgaga
      961 accggtgggg cgccggccac cgggccgcat cggcgatcag acgatgcacg gtgtcggtaa
     1021 ggtcactctg gccgagttcc gggagcgctc gctcaacgaa ctcttctccg ccgctgaata
     1081 tcgccgcaag cgccagatag agcgccgtcc cctcgcgcag ggaaagcagt tcgcgcccgg
     1141 tgaggtcgga ggtgccgacc ggctcttccc cttcgcccgc cgcggcaaga cctttcgcca
     1201 ccttcgcgag gtgggcgtgg gatcggcaca ggacggtgat gtcgccccat gcgcacccgg
     1261 tctcttcccg tcggcgccgt atctcggcaa cgagccgctc gtacagttcc ccgtcaccac
     1321 tcccgcagga caccgggatc accgcgaccg acgaaagccc ggtgtacggg tccctccgtt
     1381 cctcttcggg cggcgcctcc tgtgtatcgc ccgcgctctc gttcccggcg aaaagttcct
     1441 ttgtcagttc gttgaagaac ccgatgagga gcggggtcga gcgccaattg tagagaagtt
     1501 tctcggtcgg cagttgtccc gacctccgga gcgggtccag ccagcgttgg agccgttccg
     1561 gatcggcgcc gcgccagcga taaatggatt gtttccaatc gcctacgaaa aagagcgacc
     1621 gctcccccgc gccgttctcg ccgatgtcgc ccgcgatatc ttccagaagc ggtcgcaaca
     1681 gttccatctg tatcgccgag gtgtcctgaa attcgtcgag gagcaggtgg gcggtccggt
     1741 ggtaaccccg ttcgtagagc cgcgccgaga gttcgggacg gtcggggctg tcggacccgt
     1801 ccagagcgat cagttcgcgc agcacatcct cgaaataacg gcggtcgagt ctccgttttt
     1861 cggtctcgac cgactcggcg cgcagagcgc tgaagcgttt gaggagcgcg gtccggagcc
     1921 gcgcggtgtt gacgacatat tcggcaacga gccgccgcat cggcggatag agcgccctga
     1981 gtttcgtata ggcggcgagt ttctggtcgg cggctgacac cttgaccccg gcgatgtcgt
     2041 gcttgaggaa cagcgccttg gaaagatcga tcttgtcgag cgatcgcgac agcggtatga
     2101 caacctgttt gagtagattg cccgccgtca ccgcgccgac cgccgtgacc tctttccgca
     2161 gtttctcgta agcggcgtat atgcgtgcct gcttggcctt gagttcatca aggagtccta
     2221 tcgggcagtt ccacgccgcg agtgcgtccc gatcgttgtc gagttcgcag gtcagtcgtt
     2281 ccggcgttat cttgagtatg cgcgccgcga tgagtatctc ctcggtggcg tcgatcacct
     2341 ccgggcgggt gaagaagcgt tcgtcggcaa gcgccttgat gaggtccttt tcatggtcgt
     2401 cggccatctg cggcgcccga tcctcggtcg cgaggaactg gtggaacagc gcgtctatcg
     2461 tggaatagcg gacctgcgcc gctcgcatga gaagacgcag tctctcgacc ggtggggtat
     2521 cctcgcccgc gatcctgtcg agtatccgtt tctccatctc ggccgccgcc gccctcgtga
     2581 aggtcaccgc gacgatgagc gccgggtctc ccgcgataag gccgcgcacc accttggtca
     2641 actggtaggt cttccccgcg ccggccgagg cggtcatcag gagacttccg ttcattccgg
     2701 cctcccgacg aagtagaggt cgggtcgcag gcagagcgcc ttgtagggac agtaggaaca
     2761 cgcccgggcc ttatggttcg gcgcaaagcg ttcctgcgcg atggtccggt ccagccgccg
     2821 cgcgagcgca tccagcgttt cccgtcgccg ttcgatctcc ccggccggga gttcgacgat
     2881 atagttcccc cggtcaccgc tccgcaggca gatataggcg gcgtcgagtt catcttcttt
     2941 cgcacgaccg ttctcgataa ggagttgggc atagaaaagc agttgaaaat gatcgtgcag
     3001 gcccctctcg tcggcggcct cggcgataag cccctccttg cagacggctg gcatgctctc
     3061 cttgtatttg agatcggcaa cgagtttccg cccgttgcgt tcctgcaccc ggtcgagccg
     3121 cccggtaaga cgatacccgc cgaaggtcgc ctcgagcggc agttcggtgt cggtcaccgc
     3181 gttcgagaaa aggaccgcgg caccactttc acgctcccac gcggcgatat cgttcaggta
     3241 agcgtggacc aacgccttgc ggacggcgcg gtcggggacc ttcgtcgata tctcggcgtt
     3301 gctttcccac cgttcttcga acagttgtcg ccaccggtcg atcggcttgt cgaccccttt
     3361 gagcgacgcg aaaagatcgt gcaggaactg gccgatgacc atgttgacat ggtcctctcc
     3421 ctcgaaacag gcgggcggtt ctatattgaa gaagtctttg cgctcgatga cgaaacggaa
     3481 gggacacgaa aggaacagcg gtagcgacga gaacgaccac gggtagttcg cgagtctctc
     3541 tctcatctcg tcgctgttct tgatggcggg gtcgcccgac ggcgcgatga acggggccgc
     3601 cgccatgagt cgttttaccg gcttcatgcc gaattcgacc gacaggaacg cgaagtgggg
     3661 cgacggcgcg gcgcccgccc cggtgtcata gcgcgccgcg aggtgggcgc tctcacctaa
     3721 ggcgaggaac tggcggagcg tgagatcgtc ggactcgaac tgcgtcttgt ggatgcgcgg
     3781 cagatgaacg aggttgagga acggtccgtt gaacgggcgt ctcgggaaga tgtcgcgatc
     3841 catcggcagg atgagggccc gccggtaggg aacgccggtc gcgttcccca gtccgaccac
     3901 ctgcaccggg gccttccggt cgccgcgcag gtgcagtttc gccgacgcga cgaggtggag
     3961 agaaagcgtc tcccgctccg ctcccatgag cggtccaagt cttgtcatgt cgtcgatgag
     4021 cgctatcgcc ttctcgaagg cggccctttc ggcggcgtcg agatcgcgcc aacgtgccgt
     4081 gagttcggcc gccagttcgc ggcgcacccc gtcgagcggt cgcctctccc gtatcgccat
     4141 gatcaggcgg tgggcggcga cgaaatgcga aaagggaagc cacggaacga agttcacgcg
     4201 gtcgccgaga ggccggaaaa ggagttgcca caggtagaat gacagggtct cgtcgagtgg
     4261 cacgatgagg agttgcccct tgtttcctcc ttccccccct tgcaggaacc gctcgatctc
     4321 ggcggcgatg agcgcccgca ccgcggggcg ctcgttcaac tcggtcagtt ccagccgacc
     4381 ggtggtcgcc cgctcttcgc cgatgccgag gcgacggatc agcgcgagcg ccgaatcgta
     4441 gtcgggcgtc ccctgcggca tgtgcgggcc gaacagcggc gcatcaatga atcggcgatc
     4501 gtgcggtatg cgggaaatga accgttcgtg cgtcggggtc atgaccggca ggccgacgaa
     4561 cagttcatta tcgcgcagcg tcagatcggc gaaagccgcc tcttcgaacg gcgcgtagaa
     4621 cagcccctcg gtggccaatt ccctgcggaa ggtccccatc gtcgcgaaga agatcgctat
     4681 ccgctccata tgttcggggg cgatgcggtc gagtcgcgcg agttcgtcgc cggtgacc
//
LOCUS       DAOQUE010000199         4721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_20116_length_4721_cov_3.763395, whole genome shotgun sequence.
ACCESSION   DAOQUE010000199 DAOQUE010000000
VERSION     DAOQUE010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20116_length_4721_cov_3.763395"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PKH10_07095"
     CDS             complement(<1..986)
                     /locus_tag="PKH10_07095"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HNT27927.1"
                     /translation="MCGIVAYTGPGEGIPLVIEGLSRLEYRGYDSAGLAFFHEGRIVI
                     ERAAGKLDKLKAKVGADQPSSHAVIGHTRWATHGRPTEINAHPHVSGKTALVHNGIIE
                     NYLELREYLTGKGMNGFYSDTDSEVIAHLINHFYKGSVPEALAEAVAMIEGSYALAIL
                     HEDHPGEIYLARRSSPLVAAVSSEGGFAASDIPAVLKHTRDFVFLEDDDIAVLTSAGV
                     TVMDRSLKKVERKVHHIDWSAGAAEKGGYRHFMLKEINEQDRAVMDTLRGRLNRAQAD
                     IMLFDGDTRFFKGRKKIFLVACGTSWHAALVGKYYIEGLARVPVEVDLASEFR"
     gene            complement(1071..1694)
                     /locus_tag="PKH10_07100"
     CDS             complement(1071..1694)
                     /locus_tag="PKH10_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27928.1"
                     /translation="MASSQNTMVFSSLSAEEERQLLEFQKEAFYFTGEDFANFKRWQE
                     FYNLFFLYHVRSGDGETTVAQRRSMEPARLMLYMPSLFDRFKGTDLFSAQKLVFDQNE
                     ARDLSWFREREMILSVFAFEGERIRFVHEKASFVIELRHKGFFKKLGKLIAATPAFQN
                     HVYLLFLISVLLLKKDRFASSKLFDPFRAEELEKAHLYYQKLADRIG"
     gene            complement(1703..2983)
                     /locus_tag="PKH10_07105"
     CDS             complement(1703..2983)
                     /locus_tag="PKH10_07105"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNT27929.1"
                     /translation="MRTLIRNGKIVNPRAGKSFVGSILIEDGVVTKIAAGDMNAAGAE
                     AIDATGLSVIPGLIDMHVHFRDPGFEYKEDLVSGLEAAAAGGFTAVCTMPNTSPACDN
                     IGTIEYQLKKARTEGNGVRLFPVAAASKGLKGETMAPIGELKEAGAVAISDDGKPISD
                     SSLLLRVMQYAATFGLTYLSHAEDLSLAKGGQMNESYFSTLYGLPPIPSVAESIVVAR
                     ECLMAQYLGLPVHICHVSTKAAVETIAHFKKLGVKVTAETAPHYLTLTDEAVGSYDTN
                     TKVNPPLRSESDRKALLAALKDGTIDAIATDHAPHHERDKNVEFNTAPSGMVGLETAV
                     PLLLKTVQEGHISFDRFVELFTAGYGILGIEGGVIAEGKRADLTLIAPDYQWTIDKNM
                     FRSRSRNTPFHGFHVTGAVFRTIVGGKTVFSRTY"
     gene            complement(2980..3894)
                     /locus_tag="PKH10_07110"
     CDS             complement(2980..3894)
                     /locus_tag="PKH10_07110"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNT27930.1"
                     /translation="MKHLLGIENLNRDQILHILETAVSFEEINQRQIKKVPTLKGISV
                     INFFAEPSTRTRMSFEVAEKRLSADTYNIAVSGSAITKGETLADTALNLQAMAPDCLV
                     IRHAVSGTPHMLAQIMDCSVVNAGDGFHEHPTQALLDAYTIQKKKKRLDGLKIALLGD
                     IAHSRVARSNIHLLTKTGSQVVLAGPATMMPRDVEKLGVTVTGDPKEAIKDADVVMML
                     RIQEERESGLMYPDRREYAKFFCLDDKLLAHARPDAIVMHPGPANRGVEIEDHIMDGD
                     RSVILEQVANGVAVRMAVLYLLLGGSNS"
     gene            complement(3922..4473)
                     /gene="pyrR"
                     /locus_tag="PKH10_07115"
     CDS             complement(3922..4473)
                     /gene="pyrR"
                     /locus_tag="PKH10_07115"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNT27931.1"
                     /translation="MKRKGLKVTKVVMNAEEMEKTLNRMVHEIIERVGARPHVAIVGI
                     RTRGVHIAERILDKIERAEKVKVASGTIDIALYRDDFFKGLASPEVGPSNLNFDVDGA
                     NIVLVDDVLYTGRTVRAAIDALMDYGRPASIMYAALVDRGHRELPIQPDFIGKMVQTT
                     RDEAVAVLLKEVDDEDKVVVGER"
     gene            complement(4486..>4721)
                     /locus_tag="PKH10_07120"
     CDS             complement(4486..>4721)
                     /locus_tag="PKH10_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27932.1"
                     /translation="ATKKNDETAVRSMLTSAFVEYGVRKTGQDCFYTRDESKMVTIKC
                     SYDAPVAIFGKLMHVYRMKIEVSKREEINKYFN"
BASE COUNT          853 a         1431 c         1430 g         1007 t
ORIGIN      
        1 cggaactcgc tcgcgaggtc cacctcgacc gggacccggg caagtccctc gatataatac
       61 ttgccgacga gcgccgcgtg ccacgaggtg ccgcaggcga caagaaatat ctttttgcgc
      121 cctttgaaga accgggtatc gccatcgaac agcatgatgt cggcctgcgc ccggttgagc
      181 cgcccgcgca gggtatccat cacggcgcgg tcctgctcgt tgatctcctt gagcatgaag
      241 tggcggtatc cccccttttc cgccgccccg gcgctccagt cgatgtggtg tacctttcgc
      301 tcgacctttt taagtgaccg gtccatgacc gtgacgcctg cgctcgttag caccgcaatg
      361 tcgtcgtcct cgaggaacac gaaatcgcgg gtgtgcttga gcaccgccgg aatgtctgag
      421 gcggcgaagc ctccttcgga cgacacggcg gcgacgagcg gcgaagagcg gcgcgcgagg
      481 tatatctcgc cgggatggtc ctcgtgaagg atggcgagcg cgtaactccc ctctatcatc
      541 gcgaccgctt cggcgagcgc ctcgggaacg ctccccttgt agaaatggtt gatgaggtgg
      601 gcgatgacct cactgtcggt gtcggaatag aagccgttca tccccttccc ggtgaggtat
      661 tcgcgcagtt cgaggtagtt ctcgatgatg ccgttgtgga cgagcgccgt tttgcccgac
      721 acatgggggt gtgcgtttat ctcggtcggg cggccgtggg tcgcccagcg ggtgtggccg
      781 atgacggcgt gagacgaggg ctggtcggcg ccgaccttcg ccttaagttt atcaagttta
      841 ccagcggccc gctcgataac gatgcgaccc tcatggaaaa aggcgaggcc ggccgagtcg
      901 tagccgcggt actcaagccg tgagagtcct tcgatgacca gcggtatccc ctcgccgggt
      961 ccggtgtagg ccacgatgcc gcacatggag agccaccctc atttgcaaaa atagcagtgg
     1021 attataccta aagcgcgccc gcaagcaaaa ataaatttca gcggaaaaag tcacccgatc
     1081 cggtcggcca gtttctggta gtagagatgg gccttttcga gttcctctgc gcggaatggg
     1141 tcgaagagtt tcgacgaggc gaaccggtcc tttttgagca ggaggaccga tatgaggaac
     1201 agcagataga catggttctg gaaggcgggg gtcgccgcta tgagtttgcc cagtttctta
     1261 aaaaagcctt tgtgccggag ttcgatgacg aacgacgcct tctcgtggac gaaacggatg
     1321 cgctccccct cgaaggcgaa gaccgacagt atcatctcgc gctccctgaa ccaactcagg
     1381 tcgcgcgcct cgttctggtc gaacacgagt ttctgcgccg agaaaagatc ggtacccttg
     1441 aagcggtcga agagcgacgg catatagagc atgaggcgcg ccggctccat cgaacggcgc
     1501 tgagctaccg tcgtctcccc gtcacccgac cggacatggt aaaggaaaaa gagattgtag
     1561 aactcctgcc accgcttgaa gttcgcgaag tcctcgccgg tgaaataaaa ggcctctttc
     1621 tggaattcga gcagttgccg ctcttcctcc gccgaaaggg aggaaaagac catcgtgttc
     1681 tgcgaagagg ccatgcgaac tcctagtagg tgcgggagaa caccgtcttc ccgccgacga
     1741 tggtgcggaa caccgcgccg gtcacatgga agccgtggaa cggggtattg cgggaacggg
     1801 agcggaacat gttcttgtct atcgtccact ggtaatcggg cgcgatgagc gtgaggtcgg
     1861 cccgcttccc ttcggcgatg acgccacctt cgatacccag tatgccgtac ccggcggtga
     1921 acagttcgac gaaccggtcg aaggatatat ggccctcctg taccgtcttg agcaggagcg
     1981 gcaccgcggt ctcgagcccg accatccccg acggcgcggt attgaattcg acattcttat
     2041 cgcgctcgtg gtgcggcgcg tggtcggtcg ctatcgcgtc gatggtgccg tccttgagcg
     2101 ccgcgaggag tgccttgcgg tcactctccg agcggagcgg cgggttgacc ttggtgttgg
     2161 tgtcgtacga gccgaccgcc tcgtcggtga gcgtcaagta atgcggcgcc gtttcggcgg
     2221 tcaccttgac gccgagtttc ttgaagtggg cgatcgtctc gaccgccgcc ttggtggaga
     2281 catggcagat gtggaccggc aggccgagat actgggccat caggcattca cgcgccacaa
     2341 cgatggattc ggcgaccgac ggtatcggcg gcagaccgta gagcgtcgag aaataacttt
     2401 cgttcatctg gccgcctttc gcaagcgaga ggtcctcggc gtgcgagaga taggtcagcc
     2461 cgaaagtggc ggcgtactgc atgacgcgga gcaggagcga actgtcgctt atcggcttgc
     2521 cgtcgtccga tatcgccacc gcgcccgcct ctttgagttc cccgatgggg gccatggtct
     2581 cgcccttgag cccttttgag gccgccgcga cagggaacag ccgcacgccg ttaccttcgg
     2641 tacgcgcctt tttcaactgg tattctatgg tgccgatgtt gtcgcacgcg ggactcgtgt
     2701 tgggcatcgt acagaccgcc gtgaagccgc cggccgccgc cgcttcgagt cccgacacaa
     2761 ggtcttcctt gtactcgaag cccggatcgc ggaaatggac atgcatatcg atgagcccgg
     2821 gtatgaccga cagcccggtc gcgtctatcg cctcggcgcc ggcggcattc atgtcgccag
     2881 ccgctatctt cgtcaccacg ccatcctcga tgagtatcga gccgacgaag ctctttccgg
     2941 cccgcgggtt gactatcttg ccgttcctga tgagcgttct catgagttcg atcctcccag
     3001 cagaagatac aataccgcca tgcgcaccgc cacgccgttg gccacctgtt cgagtatcac
     3061 cgaacggtcg ccgtccatga tgtgatcctc tatctcgacg ccacggttgg ccggccccgg
     3121 gtgcatcacg atggcgtcgg gcctcgcatg cgcaagcagt ttgtcgtcaa ggcagaagaa
     3181 cttcgcgtat tcccggcggt cgggatacat gagcccgctc tctcgctctt cctgtatgcg
     3241 gagcatcatg acgacatcgg catccttgat cgcctctttc gggtcgcccg tcactgtgac
     3301 gccaagtttc tcgacatcgc gcggcatcat cgtcgccgga ccggcgagca ccacctgtga
     3361 accggtcttc gtgagcaggt ggatgttcga acgggcgacg cggctgtggg cgatgtcgcc
     3421 cagaagcgct atcttgagcc cgtcgagccg ctttttcttt ttctgtatcg tataggcgtc
     3481 gagcaatgcc tgcgtcgggt gctcgtggaa cccgtcaccg gcgttcacca ccgagcagtc
     3541 catgatctgg gccaacatgt gcggcgtccc cgataccgcg tggcgtatca ccaggcaatc
     3601 gggggccatc gcctgcaggt taagcgcggt atcggcgagc gtttccccct tggtgatcgc
     3661 gctacccgac acagcgatgt tgtaggtgtc ggccgacagc cgtttttccg ccacctcgaa
     3721 cgacatacgg gtgcgggtcg acggctcggc gaagaaattg atgacgctga tccccttgag
     3781 cgtcggcacc ttctttatct ggcgctgatt tatctcctcg aacgacaccg ccgtttcgag
     3841 gatgtggagg atctggtcgc ggttcaggtt ctcgatgccc agcagatgct tcatgaccta
     3901 tcgtctcctt tctgtgaaaa cttaccgttc gccgacgacg accttgtcct cgtcgtcgac
     3961 ctccttgagg agcaccgcga ccgcctcgtc gcgcgtggtc tggaccatct tgccgatgaa
     4021 atcgggctgg atgggaagtt cgcggtgacc acggtccacc agcgccgcgt acatgatgga
     4081 cgcgggacgg ccgtagtcca tgagcgcatc tatcgcggcg cgcacggtgc ggccggtgta
     4141 gagcacatca tccacgagta cgatattggc gccgtccaca tcgaagttaa ggttcgatgg
     4201 cccgacctcg ggactcgcga ggcccttgaa gaaatcgtcg cggtagagcg cgatgtctat
     4261 ggtgcccgac gcgaccttga ccttctccgc ccgctctatc ttgtcgagta tccgctcggc
     4321 gatgtggacg ccgcgcgtgc ggatgccgac gatggcgaca tgcggccgcg cccccacccg
     4381 ttcgatgatc tcatgcacca tgcggttgag cgttttttcc atctcttcgg cattcattac
     4441 gaccttggtg accttgagcc ccttgcgttt catcggtcgc tccgttcagt tgaagtattt
     4501 attgatctct tcgcgtttcg agacctcgat cttcatgcgg tacacatgca tcagcttgcc
     4561 gaagatcgcc accggcgcat cgtaggagca cttgatggtc accatcttgc tctcgtcccg
     4621 ggtatagaag cagtcctgcc ccgttttccg taccccgtac tcaacgaacg cgcttgtcag
     4681 catggagcgc accgccgtct cgtcgttctt cttggtggcg t
//
LOCUS       DAOQUE010000200         4701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_20250_length_4701_cov_4.192639, whole genome shotgun sequence.
ACCESSION   DAOQUE010000200 DAOQUE010000000
VERSION     DAOQUE010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20250_length_4701_cov_4.192639"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PKH10_07125"
     CDS             <1..922
                     /locus_tag="PKH10_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27933.1"
                     /translation="DALHAFLSTCDRHRFDVLLAAGYEGQSVFQHIVDRDRRDAMIAA
                     YPLELTAPTDDNPFFFNMLKPSALLQYRGIEKEGPLNTNLGAVRNLFILLGIVTVLAI
                     LLVIVPILSAMERKSAAMLRHPWSVYFAAIGLGFMLIEIALIQKFTVFLGHPTYSLLV
                     VLCTILLFTGFGSWLAGRLKGKLKPVHIFILLIAVVTAAGGVNMFVLPHLSGLPLTVR
                     ILYSAGVMAAIGTVLGMPFPTGMASLEGEQKEWGPWLWGINGALGVVSSVLATALSLF
                     FGISVTFFVGVAAYLLALAACIAAVGVRRP"
     gene            complement(985..1713)
                     /locus_tag="PKH10_07130"
     CDS             complement(985..1713)
                     /locus_tag="PKH10_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27934.1"
                     /translation="MKNQINYNLSPFSNYILRLFLLLLIIVPINVYSGKKHTYLLENG
                     VECHGEPAGETSPKIDLKEQGILGEKMENIQKMFGKTEMFDPNSSSAISPEVLCYASE
                     DDKDKTLLVIESGPMGGFGRTVSSYAIYADKGDFPHYQRCLKTKKVTRNISTQNGISL
                     STTKDNAKNILGSKITCDVKGHLLYWYDKKIVMSEEQYQKHVRGHENDIPQPSNFYFR
                     SAFILLEYRDIYIKKISVGAFEST"
     gene            complement(1741..2703)
                     /locus_tag="PKH10_07135"
     CDS             complement(1741..2703)
                     /locus_tag="PKH10_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNT27935.1"
                     /translation="MEKNAKIYVAGHTGLVGGALWRDLQKKGYTNLVGRTLAELDLRD
                     RRTVDAFFATEKPEYLLLAAARVGGIVANNTYRADFIYDNLLIELNLIDAAHKHGVKK
                     LLFLGSSCIYPKLAPQPMTEPALLTGELEYTNEPYAIAKIAGIKLCESYNIQHGTNFI
                     AVMPTNLYGPGDNFDLQTSHVMPAMIRKFHEAKQRGDKAVTLWGTGTPKREFLYVDDL
                     ADACVFLMEKVNFADLSKGMKEVRNTHINIGTGVDLTIRELAELVKKTVGFTGDILWD
                     TTKPDGTPRKLLDVSKLQSLGWKPKTMLEEGVRKAYDWYCANKA"
     gene            complement(2708..3850)
                     /gene="gmd"
                     /locus_tag="PKH10_07140"
     CDS             complement(2708..3850)
                     /gene="gmd"
                     /locus_tag="PKH10_07140"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNT27936.1"
                     /translation="MTKTALITGITGQDGAYLADLLLEKGYTVHGIKRRSSLFNTDRI
                     DHLYQDRHKRAVKFFLHYGDLTDATNLIRIVQEVQPDEIYNLAAQSHVKVSFETPEYT
                     ANADALGTLRLLEAIRILGLEKKTRFYQASTSELYGLVQETPQKETTPFYPRSPYAAA
                     KLYGYWITVNYREAYGMFACNGILFNHESPVRGETFVTRKITRAAVRIALGLQKTLYL
                     GNLGAKRDWGHAKDYVRAMHLMLQHDTPEDFVIATGRTTEVREFVRMAFDGIGITVEF
                     DGEGARETGVVAEIDEERLIAAGGKKGAVKKGATVVKVDPRYYRPTEVDLLVGDASRA
                     RKKLGWKPEHTLEELVQEMVTADMAEARRDELLKEHNYRIRLSEGD"
     gene            4089..>4701
                     /locus_tag="PKH10_07145"
     CDS             4089..>4701
                     /locus_tag="PKH10_07145"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNT27937.1"
                     /translation="MKRSLLAMAMLLLLTGVLGADTPYPLLPDPGSRLNIPEFLFGGW
                     ATDPLGPTGEELQPFIVNGQETNYQMYKGVVGLILNSSMGQSLCTATLIDPEVLLTAG
                     HCVYLKENGVIVMNAVNNPGSITVKSGANIMYGGTTIAQAEQVVKHDTWTGDINSYGG
                     LNADIALIKLDRQITDVEYYGIRTDPEENIGDPGIIVGYGITKT"
BASE COUNT          868 a         1373 c         1337 g         1123 t
ORIGIN      
        1 cgacgcgtta cacgccttcc tttccacctg cgaccgccac cggttcgatg tcctgctcgc
       61 ggcggggtac gagggacagt cggtgttcca acacatcgtc gaccgcgacc ggcgcgacgc
      121 gatgatcgcc gcctacccgt tggaactcac cgcgccgacc gacgacaacc ccttcttctt
      181 caatatgctc aaaccctcgg cgcttctcca ataccgcggc atagagaaag aaggcccgct
      241 caacaccaac ctcggcgcgg tgcgcaacct tttcatcctg ctcggcatcg tgacggtgct
      301 cgcgatcctg ctcgtcatcg tgccgatact ctcggcgatg gaacggaaaa gcgcggcgat
      361 gctgcgccac ccgtggagcg tttacttcgc cgccatcggg ctcggcttca tgctcatcga
      421 gatagcgctc atccagaagt tcaccgtctt cctcggccac ccgacctaca gtctgctcgt
      481 ggtcctctgc acgatactgc tgttcaccgg cttcggcagt tggctcgcgg ggcggctgaa
      541 agggaaactg aagccggtcc atatcttcat cctgctcatc gcggtggtga cggcggcagg
      601 cggtgtgaac atgttcgtac tgccgcacct ttcggggctg ccgctcacgg tgcggatact
      661 ctacagcgcc ggggtgatgg cggccatcgg tacggtgctc gggatgccgt tcccgaccgg
      721 catggcgtcg ctcgaaggcg aacagaagga atgggggccg tggctgtggg ggatcaacgg
      781 ggcgctgggc gtcgtctcat cggtcctcgc gacggcgctg tcgctctttt tcgggatatc
      841 ggtgaccttc tttgtcgggg ttgcggccta cctgctggcg ctcgccgcct gcatcgccgc
      901 cgttggggtc aggcggccgt aatatctaac gattttctgg atattgagaa ccaaccaccc
      961 gtctctctct tctcttttac accttcatgt cgattcaaaa gcgccgaccg agattttttt
     1021 aatgtagata tctcggtact ccagaaggat gaatgccgat ctaaaataga aatttgacgg
     1081 ttgaggaatg tcgttctcat gacccctaac atgtttttga tattgttctt ctgacatgac
     1141 gatctttttg tcgtaccaat aaagcaaatg acctttaaca tcgcatgtga ttttactgcc
     1201 caagatgttt ttggcattgt cctttgtggt agaaagagaa attccatttt gcgtcgagat
     1261 gttccgtgtg actttttttg tctttaagca tctttggtaa tgtggaaaat cgcccttgtc
     1321 agcataaatg gcataactgg aaactgttct gccaaacccg cccatgggtc cagactcaat
     1381 caccaaaagg gttttatctt tatcgtcttc cgaggcatag cataacactt ctggactaat
     1441 ggcactggaa gagtttggat caaacatttc tgttttacca aacatctttt gtatgttctc
     1501 cattttctcg cccaaaatac cttgttcctt taaatctatt ttgggggaag tctcgcccgc
     1561 aggttccccg tggcactcta ccccattttc aagcaaataa gtgtgctttt tacccgaata
     1621 aacattgatg ggtacgatga ttagaagtaa caaaaaaaga cgcaggatat aattagaaaa
     1681 aggagacaag ttataattaa tctggttctt catgggccac ccccgcccga ttttatgtcg
     1741 tcaggcttta ttagcgcagt accagtcgta ggctttccgg acaccttctt caagcattgt
     1801 cttgggtttc cagccgagcg actgcagttt cgagacatcg agcagtttgc gcggcgtccc
     1861 gtcgggcttg gtcgtgtccc agaggatatc gcctgtgaag ccgaccgtct ttttcaccag
     1921 ttcagccagt tcgcgtatcg tgagatcgac gccggtgcca atattgatat gggtgttgcg
     1981 cacctctttc atccccttcg acagatcggc gaaattcacc ttctccatca ggaagacgca
     2041 ggcgtcggcg aggtcatcca catagaggaa ttcgcgcttc ggcgttccgg tcccccagag
     2101 cgtcaccgcc ttatcgccgc gctgtttcgc ctcgtggaac ttccgtatca tcgcgggcat
     2161 cacatgactc gtctgcaggt cgaagttgtc gccgggtccg tagagattgg tcggcattac
     2221 cgcgatgaag ttggtcccgt gctggatatt gtacgattcg cagagtttga tccccgctat
     2281 cttcgcgatg gcgtagggtt cgttggtgta ttcgagttct cccgtgagta gcgccggttc
     2341 ggtcatcggc tggggcgcca gtttggggta gatgcacgac gagccgagga agagcagttt
     2401 cttcaccccg tgtttgtggg cggcgtcgat aagattcagt tcgatgagca ggttgtcgta
     2461 gatgaagtcg gcgcggtagg tgttgttcgc gacgatgccg ccgacgcggg ccgccgcgag
     2521 gagcagatac tccggctttt cggtggcgaa gaaggcgtcg accgtccgcc ggtcgcgcag
     2581 gtcgagttcg gcgagcgtcc ggccgacgag gttcgtgtag cccttcttct gcaggtcacg
     2641 ccacagcgcc ccgccgacca gcccggtgtg ccccgcgaca tatatcttgg cgttcttttc
     2701 catcgatcta gtccccttcg ctcaggcgga tccggtagtt gtgttccttg agcagttcgt
     2761 cgcgccgcgc ctcggccatg tcggcggtga ccatctcctg caccagttct tcgagcgtat
     2821 gttccggctt ccagccaagt tttttgcgcg cccgcgacgc gtcgccgacg agcagatcga
     2881 cctcggtcgg ccggtagtag cgcgggtcga ccttcaccac cgtcgccccc ttcttgaccg
     2941 cccccttctt gccgcccgcc gcgatgagtc tttcctcatc gatctccgcc accaccccgg
     3001 tctcgcgtgc cccctcgccg tcgaactcga ccgttatccc gatgccgtcg aacgccatcc
     3061 gtacgaattc gcgcacctcg gtggtgcgac cggtcgcgat gacgaaatcc tcgggcgtgt
     3121 cgtgctggag catcaggtgc atggcgcgga cataatcctt cgcgtggccc cagtcgcgct
     3181 tcgccccgag gttgccgagg tagagcgtct tctgtaggcc gagcgcgata cggaccgcgg
     3241 cgcgcgttat cttgcgggta acgaaggtct cgccgcggac cggcgattcg tggttgaaca
     3301 gtatcccgtt gcaggcgaac atcccgtagg cctcgcggta gttcaccgtt atccagtagc
     3361 cgtacagttt cgcggcggcg tagggactgc gcgggtagaa gggtgtcgtc tccttctgcg
     3421 gcgtctcctg cacgagcccg tagagttccg aggtcgacgc ctgatagaac cgggtcttct
     3481 tctcgaggcc gaggatgcgg atcgcctcaa ggagccgcag ggtgccgagc gcgtcggcgt
     3541 tcgcggtgta ctcgggcgtt tcgaacgaca ccttgacatg cgactgcgcc gcgaggttgt
     3601 atatctcgtc gggctggacc tcctgcacga tgcggatgag gttggtcgcg tcggtcaggt
     3661 cgccgtagtg gaggaagaac tttaccgccc gcttgtgccg gtcctgatag agatggtcga
     3721 tgcggtcggt gttgaacagc gacgaccgcc gcttgatgcc gtggaccgtg tatccctttt
     3781 cgagaagaag gtcggcgaga taggcgccgt cctgcccggt gatgccggtg atgagtgccg
     3841 tcttggtcat gggatattcc ccctattcgg ttgctcgggc gattatagcg cgtcgcccct
     3901 tttaggaaag ttttccctgc gtttctcttt tctatcgaac cgctgttagt tgttaacata
     3961 ttgatataaa tgatgatatg gtcacggaac gccctttgct caacaaagag gttgcaaaga
     4021 ggcgtgatgg aacttatact gtccctcgct ggtctaatga ctgtggaacg cgttagagga
     4081 ggtatcttat gaaacgatcg ttgctcgcga tggcgatgct gctgcttctt accggtgtcc
     4141 tcggtgccga taccccgtac cccctgcttc ccgatccggg atcgcggctc aatatccccg
     4201 agttcctgtt cggcgggtgg gcgaccgacc cgctcggtcc gaccggcgag gaattgcagc
     4261 cgttcatcgt caacgggcag gagaccaact atcagatgta caagggggtc gtcggcctca
     4321 tactcaactc ctcgatgggg cagtcgctct gcaccgcgac gcttatcgac ccggaggtcc
     4381 tcctcacggc gggtcactgc gtctatctca aggagaacgg ggtcatcgtg atgaacgcgg
     4441 tgaataaccc cggttccatc accgtcaagt cgggcgccaa catcatgtac ggcggcacca
     4501 cgatcgcgca ggccgagcag gtggtcaagc acgacacttg gaccggcgac atcaactcct
     4561 acggcgggct gaacgccgac atcgcactca tcaaactcga ccgccagata accgatgtcg
     4621 agtactacgg catccgcacc gatcccgagg agaacatcgg cgatcccggc atcatcgtcg
     4681 gctacggcat caccaagacc g
//
LOCUS       DAOQUE010000201         4691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_20320_length_4691_cov_4.132657, whole genome shotgun sequence.
ACCESSION   DAOQUE010000201 DAOQUE010000000
VERSION     DAOQUE010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20320_length_4691_cov_4.132657"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PKH10_07150"
     CDS             complement(<1..710)
                     /locus_tag="PKH10_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27938.1"
                     /translation="MSRRAIAIVAVALMLVAAPVYITARSHIIDHPKPVVETIAAYPR
                     PEFARVMSLGFNAMMADFLFIKAAYYFGTHYVTDRTYTLLGRMIEVIARLNPDLKIAI
                     LFGDAAISSMRTPEAITEANRLLDIGHELYPDDWNFVFRKGMNYYLYLDDMERAYPFL
                     YKGARMPGAPEKAYWLVTKAMTKGGGYHLAYDYTKDQWEQAKDKHMRDMLETRLRFYT
                     ELIILSEAAQRFLEKEGRS"
     gene            complement(703..1617)
                     /locus_tag="PKH10_07155"
     CDS             complement(703..1617)
                     /locus_tag="PKH10_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT27939.1"
                     /translation="MQVSVFIPFQNERDNIAEVIERVERGLARAGVTGELVMVDDGSD
                     DGGDAVVENIAREKPWVRLLRHRRNLGLTRAMKTGFAACTGDIIVFLPSDLESHPDED
                     IPALLAGFRDGVDIVCGRRKHRRESKIFLSWMYNEISYALFGIRLHDMNWIKAFRRAC
                     VADMELRSDWHRFMVQILHLKGWKAVEVPVEWHPRRSGRSHFGFMRIPISFFDALVVR
                     FLITFTKSPMRLFGSFGGLQMITSVAIIVWMLYMTFAFNDSVFRNRPLLLFTVGMFLS
                     GTIFLFMGFLAELVVGLKDDIDRRDRRE"
     gene            complement(1620..1937)
                     /locus_tag="PKH10_07160"
     CDS             complement(1620..1937)
                     /locus_tag="PKH10_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HNT27940.1"
                     /translation="MSTLNGSQRKHLRGLAHALRPVVLVGLKGFTPELARSLDTALLA
                     HELIKVKFLDHKDEKKDIAERMAAASGAEIAGIIGNIAILYRPHPDPAKRRIALDKKT
                     TED"
     gene            complement(1934..2860)
                     /locus_tag="PKH10_07165"
     CDS             complement(1934..2860)
                     /locus_tag="PKH10_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNT27941.1"
                     /translation="MGLDRYVNTFNRYLRERFGERVARISFDTGIPCPWGKCVFCDNR
                     VFVPPTAIVPEGHGWEARFVAQRERAAARYGARKFIAYFQSGTSTAGDPERLGALYRR
                     AAHLDGVVGLSLSTRPDHVSEEIVRRILAAVPSPDTDIWMEIGLQSIHPRSLAFLNRG
                     HDAADYDRAVEMIHRVGGDRIKVSAHLIAAIPGETTDDLLETVRHATAHPAVRGVKIH
                     HLQVYRETPLAALFEREPFGLPGEEEYIALLGDILRALPPRIVVMRLFTDAPDDRLIA
                     PRWRSGTQELLRKLEEHCEKHTIRQGNNGVPA"
     gene            2968..3043
                     /locus_tag="PKH10_07170"
     tRNA            2968..3043
                     /locus_tag="PKH10_07170"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3001..3003,aa:Thr,seq:cgt)
     gene            3187..4155
                     /locus_tag="PKH10_07175"
     CDS             3187..4155
                     /locus_tag="PKH10_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27942.1"
                     /translation="MKHLAVMLAVAFIALSLAAQEKKPKVAVMEMHDLSGKLDKAMLE
                     TATEVLRSELVATNRFVVISKERQRKETVKEAKKESWKECYDQSCRIELGQALSADSI
                     LSTTITLFGGTYTITAELVDLAKEATVAGAKAQFDGTEKELGRAITEITGRLVGKEAR
                     PAAEASPAVVAPSAGRSADLRAEELYNKGRDLEATDRERALELYRDAMELVPTGSRYH
                     ERAKERVIKLTAAPTVREGNYEVVGYNPNGSQYRGSLTIKRQGDLYYLTWYIGNNTFN
                     GKGYLKGKTLTIDWGEQYPVIYTVQENGTLKGTWANGTASETLTPK"
     gene            complement(4184..4537)
                     /locus_tag="PKH10_07180"
     CDS             complement(4184..4537)
                     /locus_tag="PKH10_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27943.1"
                     /translation="MPDLTDILILLALNLLLWTPLLILKHPIFIVTERLPRLRGDFPA
                     GVSWLFLVFVRKGFDSAELRRHEEVHFRQWRRYSPLVMGALYLGSSLYWLIRTRSLWG
                     AYWHNRFEVEARKAP"
     gene            complement(4525..4677)
                     /gene="cutA"
                     /locus_tag="PKH10_07185"
     CDS             complement(4525..4677)
                     /gene="cutA"
                     /locus_tag="PKH10_07185"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HNT27944.1"
                     /translation="MKTRRDLFPQIERAMAELHPYEVPELVALPIEMGGAPYLKWLDE
                     ECVCPT"
BASE COUNT          899 a         1394 c         1534 g          864 t
ORIGIN      
        1 gaacggcctt ccttctcgag aaatcgctgg gccgcctccg agagtatgat gagttcggtg
       61 tagaaccgca ggcgggtctc cagcatgtcg cgcatgtgtt tgtccttcgc ctgttcccac
      121 tggtccttcg tgtagtcgta ggcgagatgg tagccgcccc ccttcgtcat cgccttggtg
      181 acgagccagt aggccttttc gggcgccccc ggcatccgcg cccccttgta gaggaacggg
      241 taggcccgct ccatgtcgtc gaggtagagg tagtagttca tgcccttgcg gaacacgaag
      301 ttccagtcat cggggtagag ttcatggccg atgtcgagca ggcggttcgc ctcggtgatc
      361 gcctccggcg tccgcatcga tgatatcgcc gcgtcgccga acagtattgc tatcttgagg
      421 tcgggattga gccgcgcgat gacctcgatc atccggccga ggagcgtgta ggtgcggtcg
      481 gtgacatagt gggtgccgaa gtaataggcc gccttgatga acaggaaatc ggccatcatc
      541 gcattgaagc cgagcgacat gacgcgggcg aactcggggc gggggtaggc ggctatcgtc
      601 tcgacgacgg gtttgggatg atcgatgatg tgggatcgcg ccgtgatgta gaccggcgcc
      661 gcgacgagca tgagggcgac ggcgacgatg gcgatggcgc gcctactcac ggcggtccct
      721 ccggtcgatg tcgtccttca gccccacgac gagttcggcg agaaagccca tgaacaggaa
      781 tatggtaccc gacaggaaca tgccgacggt gaacagcagg agcgggcggt tgcggaacac
      841 agagtcgttg aaggcgaagg tcatgtagag catccagact atgatggcga cgctcgtgat
      901 catctgcagg ccgccgaacg atccgaacag ccgcatcggc gattttgtga aggtgatgag
      961 gaacctcacg acaagcgcgt cgaagaacga tatcggtatc cgcatgaacc cgaagtgact
     1021 ccgaccgcta cggcgcgggt gccactcgac cggcacctcg accgccttcc accccttgag
     1081 gtggagtatc tggaccatga accggtgcca gtcgctccgc agttccatgt cggcgacgca
     1141 cgcgcggcgg aacgccttta tccagttcat gtcgtgcagg cgtatcccga agagggcata
     1201 cgatatctcg ttgtacatcc aactgaggaa tatcttcgac tcgcgacggt gtttgcgtcg
     1261 accgcagacg atgtcgaccc cgtcgcggaa cccggcgagc agggccggta tgtcctcgtc
     1321 gggatgcgac tcgagatcgc tcggcaggaa aacgatgatg tcgccggtgc aggcggcaaa
     1381 accggtcttc atcgcgcggg taagcccgag attgcggcgg tggcgcagga gacgcaccca
     1441 cggcttctcg cgggcgatgt tctcgacgac cgcatcgcca ccgtcgtccg aaccgtcgtc
     1501 caccatgacg agttcgcccg tgaccccggc gcgggcgagt ccccgttcga cccgttcgat
     1561 gacctcggcg atgttgtcgc gctcgttctg gaacggtata aagaccgata cctgcatggt
     1621 cagtcctccg tcgtcttctt gtcgagcgcg atgcgccgtt tcgcagggtc ggggtggggg
     1681 cggtacagta tcgcgatgtt gccgatgatg ccggcgatct cggcccccga cgcggccgcc
     1741 atccgttcgg cgatgtcctt cttctcatcc ttgtgatcga ggaacttcac ctttatcaat
     1801 tcgtgggcga ggagcgccgt gtcgagcgac cgcgccagtt cgggggtgaa ccccttcagc
     1861 ccgacgagga cgaccggcct gagcgcatgc gccaatcccc tcagatgttt ccgttgcgat
     1921 ccgttcaggg tgctcatgcc ggtactccgt tgttgccctg acgaatggta tgtttctcgc
     1981 agtgttcttc aagttttctc aaaagttcct gcgtccccga ccgccagcgc ggcgcgatga
     2041 gccggtcgtc cggcgcgtcg gtgaacaggc gcatcacgac gatccggggc ggcagggcgc
     2101 gaaggatgtc gccgaggagc gcgatatact cctcctcgcc ggggagtccg aacggctccc
     2161 gctcgaacag cgcggcaagc ggggtctccc ggtacacctg caggtggtgg atcttgaccc
     2221 cccgcacggc ggggtgggcg gtggcatgcc ggacggtctc tagcaggtcg tcggtcgtct
     2281 cgccgggaat ggcggcgatg agatgggccg acaccttgat gcggtcgccg cctacccggt
     2341 ggatcatctc gaccgcgcgg tcgtagtccg ccgcatcgtg cccccggttc aggaaggcga
     2401 gcgaccgtgg gtggatgctc tggagcccga tctccatcca gatatcggtg tcgggggagg
     2461 ggaccgccgc caagatgcgg cggacgatct cctcgctcac gtggtcgggg cgcgtcgaaa
     2521 gggagagccc gaccaccccg tccagatggg ccgcgcgacg gtacagcgcg ccgagtcttt
     2581 cggggtcgcc ggcggtcgag gtgccactct ggaagtaggc gatgaatttg cgggcgccgt
     2641 accgggccgc ggcccgctcc ctctgcgcga cgaaccgcgc ctcccatccg tgcccctccg
     2701 gcacgatggc ggtcggcggc acgaacacgc gattgtcgca gaatacgcat ttcccccacg
     2761 gacaggggat gccggtatcg aaactgatgc gggcgacccg ctcgccgaac cgttcccgca
     2821 ggtagcgatt gaaggtgttc acatatcggt caagacccat cggcggaggt tccttctgaa
     2881 ataataccca cgactccaca atataaacaa gagcgtctcc gcgtcaagtt tgacaaccat
     2941 tattttttga ttaccatgcc tcgcggcgct gatgtagctc agtcggtaga gcagctgatt
     3001 cgtaatcagc aggtcgacgg ttcaagtccg tccatcagct ccattgattt caacggcttc
     3061 gctccggaag gggcgaggcc gtttctgttt cttgttttgt cctgttctgt tttgtgcttg
     3121 cgcctgaaat cttgtaatcg tgacgcaatt cagctatcat cgagcgttct gatgatgggg
     3181 atcgacatga aacatcttgc cgtgatgctg gcggtcgcgt ttatcgcgct ttcgctggcg
     3241 gcacaggaaa agaaaccgaa ggtcgcggtc atggagatgc acgatctgtc ggggaaactc
     3301 gacaaggcga tgctcgaaac ggcgaccgag gtgttgcgca gtgaattggt ggcgaccaac
     3361 cgcttcgtcg tcatatcgaa ggagcgccag cgcaaggaaa cggtgaaaga ggcgaaaaag
     3421 gaaagttgga aggagtgcta cgaccagtcg tgccgcatcg aattggggca ggcgctgtcg
     3481 gccgacagca ttctttcgac caccatcacg ctcttcggcg gcacctacac gataaccgcc
     3541 gaactcgtcg atctcgcgaa agaggcgacg gtggcggggg ccaaggcgca gttcgacggc
     3601 accgaaaagg aactgggccg cgcgatcacc gagataacgg gccgtcttgt gggcaaagag
     3661 gcgcgtccgg cggccgaggc gtctcccgcc gtcgttgcgc cgtcggccgg gaggtcggcg
     3721 gacctgcgcg ccgaggaact ttacaacaag ggacgcgatc ttgaggcgac cgaccgcgaa
     3781 cgggcgctcg aactttaccg cgatgcgatg gaactggtac cgaccggaag ccgctaccat
     3841 gagcgggcca aggagcgggt gatcaaactg accgccgcgc cgacggtgcg cgaaggcaac
     3901 tatgaggtgg tcggatacaa cccgaacggg tcgcagtacc gtggatctct gaccatcaag
     3961 cggcagggcg atctttacta cctgacctgg tacataggga acaacacctt caacgggaaa
     4021 ggctatctga agggcaagac cctcaccatc gattggggcg aacagtaccc ggtcatctac
     4081 acggtgcagg agaacggcac cctcaagggt acttgggcca acggcaccgc ctcggaaacg
     4141 ctgacgccga agtgaggcga cggggcctcc tcagccgatc ccctcacggg gccttgcgcg
     4201 cctccacctc gaaacgatta tgccagtacg cgccccagag actgcgggtg cggatgagcc
     4261 agtaaagcga cgagccgaga tagagcgccc ccatgacgag tggcgagtac cgccgccact
     4321 gccggaaatg gacctcttcg tgccgccgga gttcggcgct gtcgaacccc ttgcgcacaa
     4381 agacgaggaa gagccacgaa acgccggcgg ggaaatcgcc ccggagccgg ggcagtcgtt
     4441 ccgtcacgat gaagatcggg tgcttgagga tgaggagcgg ggtccagagg aggaggttga
     4501 gcgcaaggag gatgaggatg tcggtcaggt cgggcatacg cactcctcat ccagccattt
     4561 caggtagggg gcgccgccca tctctatcgg cagggcgacc agttccggca cctcgtacgg
     4621 gtgcagttct gccatcgccc gctctatctg cgggaagagg tcgcgccgcg tcttgatgag
     4681 gcagagcgtc t
//
LOCUS       DAOQUE010000202         4673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_20456_length_4673_cov_5.033997, whole genome shotgun sequence.
ACCESSION   DAOQUE010000202 DAOQUE010000000
VERSION     DAOQUE010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20456_length_4673_cov_5.033997"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="PKH10_07190"
     CDS             <1..1094
                     /locus_tag="PKH10_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT27945.1"
                     /translation="IAHDFNNMLSVILGHTEMLFDRLKSDDPLLTDLRKIHKAAERSA
                     DLTRQLLAFARKQTIAPQVLDLNATVAGMLDMLKRLIGEDIDLAWMPGREAGRVRMDP
                     SQIDQILANLCVNARDAIVDTGKITIETGDARFDEKYCATHAGYMPGDYVLLAVSDNG
                     CGMDTVTVGQLFEPFFTTKDRGKGTGLGLSTVYGIVRQNNGFINVYSEPGRGTTFRIY
                     LPLCTERPPVEPKTEAPVATVGGGETILLVEDETMIRDIARKMLEILGYTVISAATPG
                     EAIRLAREHAGTIHLLFTDVVMPEMNGRELARNLLSLYPDMKRLFMSGYTANVIAHHG
                     VLDHGVHFLQKPFSMNDLAHKVREALAGS"
     gene            1280..2389
                     /locus_tag="PKH10_07195"
     CDS             1280..2389
                     /locus_tag="PKH10_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT27946.1"
                     /translation="MKRYLEAQIEKDLTKKMVFLGGPRQVGKTTIAKRLLGGKKGYLN
                     WDIAEDRDRILKRELPDTPLLVFDEIHKYRLWRAFLKGLFDRDAATRRILVTGSARLD
                     LYRHGGDSLQGRYHYLRLFPLSAAELGMKTPKELATLLTLGGFPEPFLSGSETEARRW
                     SREYRTRLVRDEVANLEQLRDLDAMERLMLRLPELVASPLSANALREDLQVSHKAVSH
                     WLDIFERLYAIVRIAPFGGPAIRAVKKERKHYHFDWTLVPDPGKRFENMVAIHLLKYA
                     AYREDTEGYDMEVRYFRDVDGREVDFVLLQDRKPILFVECKYGDDDIARGLRYLSAKY
                     PKVPAYQIHFEGKKDYLTPEGIHVCPAINLLREWI"
     gene            complement(2364..2903)
                     /locus_tag="PKH10_07200"
     CDS             complement(2364..2903)
                     /locus_tag="PKH10_07200"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNT27947.1"
                     /translation="MCEKKLTGFPAVVGKRPTILILGSMPGERSLTMREYYGHPQNSF
                     WRIMGTLCGAVPELPYEQRLAALKRHGIALWDVLKHCERIGSGDGDIRRESEVPNAIG
                     DLLRKHPTIRAVALNGGKARQAFRRHIWPTLPDAVTARLTILELPSTSPACAMISYKE
                     KLHRWREVERFLRSTRAEG"
     gene            complement(2910..3731)
                     /locus_tag="PKH10_07205"
     CDS             complement(2910..3731)
                     /locus_tag="PKH10_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27948.1"
                     /translation="MAKKRISDLDAQQKARAISNYLFLAFFFFVALVTICATMSGVVL
                     ADSKMYKNIMKISIAVMLLAGFVIKIVGPKIIMYFIKDKLIDEAILANHQVIADETTI
                     KKISWTDAIMSNKGRGSQIRGSLFAGIVMLDIIDALANVYSGEFYILFGTAARFEITN
                     RIFSLLQVSDFFNWKTNTLILLIAVCTSIVFVFFSLKSEEKKYFVLGLGVFFADVVWL
                     SIKDQRVSVVLHLVIFFVTFLNLIGFASQVRNGETEDDRQSADIKTIDNDSFKEF"
     gene            3878..>4673
                     /locus_tag="PKH10_07210"
     CDS             3878..>4673
                     /locus_tag="PKH10_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HNT27949.1"
                     /translation="MAIILDGSHLTIEQLVAIARNGEKVELCPKALERIKLCRAMLEE
                     KVAKREIMYGVNTGIGEFSEVVLADDQIEQFQRWLVYNHSAGIGDPAPLEHVRAAMAA
                     RINVHAHGNSGCRPEIPQTFVAMLNKGVTPFVCKKGSVGACGDLAPMSQIALTLLGEG
                     KAYYKGELLPAKTAFEKAGIAVPGLKARDGLAAINGSNLLTSISALTLYDADRWLKQA
                     EIAASMSLEALLANMKPYHTKLHELRGFAGAVRSAAAIRKCVAGSDL"
BASE COUNT         1066 a         1418 c         1249 g          940 t
ORIGIN      
        1 gcatcgcgca cgacttcaac aatatgctgt cggtcatcct cggccacacc gagatgctgt
       61 tcgaccggtt gaagtccgac gatccgttac tgaccgacct gcgcaagata cacaaggcgg
      121 ccgaacgatc ggccgacctc acgcggcaac tgctcgcctt cgcccgcaaa cagacgatcg
      181 ccccgcaggt gctcgatctc aacgcgacag tggcgggaat gctcgacatg ctcaagcgac
      241 tcatcggcga ggacatcgat cttgcgtgga tgccgggcag ggaggccggc cgggtgcgca
      301 tggacccctc gcagatcgac cagatactgg caaatctctg cgtgaatgca cgtgacgcca
      361 tcgtcgacac cggaaaaata acgatagaga cgggggacgc ccgattcgac gagaagtact
      421 gcgcgaccca cgccggatac atgcccggcg actatgttct gctcgcggtg agcgacaacg
      481 gctgcgggat ggacaccgtg acggtcgggc aacttttcga gccctttttc accacgaagg
      541 atcgcgggaa agggaccggt ctcggacttt ccaccgtcta cggcatcgtc cggcagaaca
      601 acggcttcat caatgtctac agcgaaccgg ggcgcggcac caccttccgg atatatctgc
      661 ctctctgcac cgagcggccg cccgtggagc cgaagacgga ggcaccggtc gcgacggtcg
      721 gcggcggcga gacgatactg cttgtcgagg acgaaacgat gatccgtgac atcgcccgca
      781 agatgctcga gatcctcggc tacacggtga tatcggccgc gacgccgggc gaggcgatac
      841 ggctggcgcg tgaacacgcc ggcacgatac atctcctgtt caccgatgtg gtcatgcccg
      901 agatgaacgg gcgcgaactc gcccggaacc tgctatcgct ctaccccgat atgaaacggc
      961 tcttcatgtc gggctacacc gccaatgtca tcgcccacca cggggtgctc gaccacgggg
     1021 tgcatttcct gcaaaagccg ttctcgatga acgatctggc ccacaaggta cgcgaggcgc
     1081 tcgccggatc gtaagggatc gctccacctc acgccaccgg cttaactttt ccgcatagga
     1141 tatcatcgca caagcgggac tggccaataa cattcgcgcg agttttttca cccataggca
     1201 aaaatactcg cgagtttttt tgacttcagg caaaattact cgtatcctgt gccggtagcg
     1261 aaatgggagg aaaactgaca tgaaacggta tcttgaagcg cagatcgaaa aagacctgac
     1321 gaaaaagatg gtctttctcg gcgggccacg acaggtcggc aagaccacca tcgccaagcg
     1381 cctgctcggc ggcaaaaagg gttacctcaa ctgggacatc gccgaggacc gcgaccggat
     1441 actgaaacgg gaactacccg acacgccgct cctcgtgttc gacgagatac acaaataccg
     1501 cttgtggcgc gcctttctca aaggactctt cgaccgcgac gccgccacgc gccgcatcct
     1561 cgtcaccggc agtgcgcggc tcgacctcta ccggcacggc ggcgattcgt tgcagggacg
     1621 ctaccactat ctgcggctct tcccgctgtc ggcggccgaa ctcggcatga aaacgccgaa
     1681 ggagttggca acgcttctga cgctcggcgg cttccccgaa ccgttcctgt cgggctccga
     1741 gaccgaagcg cgccgctggt cgcgcgaata ccgaacgcgg ctggtgcgtg acgaagtggc
     1801 gaatctcgaa cagctccgcg atctcgacgc gatggaacgg ctcatgctac ggcttcccga
     1861 actggtcgcc tctccgcttt cggccaacgc cctgcgcgag gacctgcagg tgagtcacaa
     1921 ggcggtgtcg cactggctcg atatcttcga gcggctctac gccatcgtcc gcatcgcccc
     1981 cttcggcggt ccggccatcc gggcggtcaa aaaggagcgc aagcactacc atttcgactg
     2041 gacgctcgtc cccgatcccg gcaagcggtt cgagaatatg gtggcgatcc atctgctgaa
     2101 atatgccgcc taccgcgagg acaccgaggg ctacgacatg gaggtgcgct acttccgcga
     2161 tgtggacggg cgcgaggtcg acttcgtcct gttgcaggac cgcaagccga tacttttcgt
     2221 cgagtgcaag tacggcgatg acgatatcgc gcggggtctc cggtaccttt cagcaaaata
     2281 tccgaaggtg cctgcctatc agatccattt cgaggggaag aaggattatc tgacccccga
     2341 agggatacat gtctgcccgg cgatcaacct tctgcgcgag tggatctgag gaaccgttcc
     2401 acttcacgcc agcgatgcaa cttctcctta taagatatca ttgcgcaggc ggggctcgtc
     2461 gagggaagtt ccagtattgt gaggcgcgcg gtgaccgcgt cggggagcgt cggccagatg
     2521 tggcggcgga acgcctgccg cgccttgccg ccgttgagcg ccaccgcccg tatcgtcggg
     2581 tgtttcctca acaggtcgcc gatggcgttg ggcacctcgc tttcacggcg gatgtcgccg
     2641 tcgcccgagc cgatccgttc gcagtgcttg aggacatccc acagcgcgat gccatgccgt
     2701 ttcagggcgg cgagtcgctg ttcatacggc agttcgggca ccgcgccgca aagtgttccc
     2761 atgatgcgcc agaaactatt ttgcgggtgg ccgtaatatt cgcgcatcgt cagcgatcgc
     2821 tcgcccggca tcgaaccgag tatcaggatg gtcggccgct tgccgacgac cgccgggaaa
     2881 ccggtgagtt ttttctcgca cataccggtt cagaattctt taaaactatc gttgtcaatg
     2941 gtcttgatat ccgcggactg acgatcatct tctgtctcgc catttcgaac ttgggaagca
     3001 aagccaatta ggtttaaaaa tgtcacaaaa aagatcacca agtgaagaac aacgctcact
     3061 ctttgatctt ttatagaaag ccaaaccaca tctgcaaaaa aaacgcccag ccctaaaaca
     3121 aaatattttt tctcctctga cttaagagag aaaaacacaa atacgataga agtacaaaca
     3181 gcaattaaaa ggattaaggt gtttgttttc cagttaaaaa aatcagatac ttgcaaaaga
     3241 gagaaaatcc ggtttgtaat ttcaaatctt gccgccgtgc caaagagaat ataaaattca
     3301 ccgctataaa cattggccaa cgcatctata atgtccagca taactattcc tgcaaaaaga
     3361 cttcctctta tttggcttcc tctcccctta ttggacatta tcgcatctgt ccacgatatc
     3421 ttctttatag tagtctcatc agctattact tggtgatttg cgagtattgc ctcgtcaatc
     3481 agtttgtctt tgataaaata catgattatt ttggggccaa caatctttat tacaaaaccc
     3541 gcaagcaaca taacggctat agatattttc atgatgtttt tgtacatttt tgaatcggct
     3601 agtacgacac ctgacattgt agcgcaaatt gtcacgagag caacgaaaaa gaaaaaagca
     3661 aggaacagat aattagaaat tgccctcgcc ttctgctgtg catccaaatc gcttattctc
     3721 tttttggcca tacggccccc atttttcccc acacatcggc atgttacgat tccgccggtt
     3781 ccttgtcaag aaagcggtca ttttccttgc ctctcctttc cttactaata taatcgcccc
     3841 cggtcggtag tgaataacct cttttaggga gatccccatg gccatcatcc tcgacggcag
     3901 ccatctgacg atcgaacaac tcgtcgccat cgcccgcaac ggcgaaaagg tagaactctg
     3961 ccccaaagca ctcgaacgga taaaactctg ccgcgcgatg ctcgaagaaa aggtcgccaa
     4021 gcgcgagatc atgtacggcg tcaacaccgg catcggcgaa ttctccgaag tggtcctcgc
     4081 cgacgaccag atagaacagt tccagcggtg gctggtctac aaccactcgg ccggcatcgg
     4141 cgaccccgcc ccactggagc atgtccgcgc cgcgatggcg gcccgcatca atgtccacgc
     4201 ccacggcaac tccggctgcc gccccgagat accgcagacc ttcgtcgcga tgctgaacaa
     4261 aggcgtcact ccgttcgtct gcaagaaagg gtcggtcggc gcctgcggcg acctcgcccc
     4321 gatgagccag atagcactga cgctcctcgg cgagggcaag gcctactaca aaggtgaact
     4381 gctcccggcc aagaccgcct tcgaaaaggc gggcatcgcg gtccccggcc tcaaggcgcg
     4441 cgacggactc gccgcgataa acgggtcgaa cctcctcacc tcgataagcg cgttgaccct
     4501 ctacgacgcc gaccggtggc tcaaacaggc cgagatcgcc gcgtcgatgt cgctcgaggc
     4561 gctccttgcc aacatgaagc cctatcatac aaaactccat gaactgcgcg gctttgcggg
     4621 cgccgtccgc tcggccgctg cgatacggaa atgcgtcgcc gggtccgacc tcc
//
LOCUS       DAOQUE010000203         4652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_20574_length_4652_cov_3.640418, whole genome shotgun sequence.
ACCESSION   DAOQUE010000203 DAOQUE010000000
VERSION     DAOQUE010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20574_length_4652_cov_3.640418"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2056)
                     /locus_tag="PKH10_07215"
     CDS             complement(<1..2056)
                     /locus_tag="PKH10_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HNT27950.1"
                     /translation="MRKCLLTALAALFLLTACDLNEAGGIRLKYSWTDAEGNDITPPD
                     TTLLHAWGELAYGTEIKTTGPAWLGGDDAKIRFDNLPYGVAMTLELTIRAADAPEKKE
                     IAPAERQDNVAYYCTSPAFKLTRGKVTVVENACIMTPAAGVDPETGKPTAPALKVYDP
                     DGKELTEEEGITRHTTVTLCYRALNATTVHIANDAGFEGATTGTIPEATGISPCPAGY
                     HAHPDWSLGDPPDPANDRAQRIVFIKASDAAGYETKPASRKVFIDNKAPIIVNQGISP
                     AAANGTSKVAIAISFHEPVQSVVLDAGDLTFTRTGDQNDTQLFIYEYQVTPATVEKDY
                     TFTVIATDLAGNPATIPLTDTLTIDKTLPDLTATIEKNDTAVEAGTTLYLKDKDTLTV
                     ALTLTEAPKETPRVNLGTYQLTCTQGADTEHYDCALTIDKDTHTEGIADLTVAFEDAA
                     GNPYAAPLKAGIRLDYTAPVLTLDFNKTAFNANDTIRINVTSTEELAGEPVVTIPAAS
                     MTLSSPTQVSGFSWLYEMSPSLFNDDTYTILADGSDKAGHPGTVDAHFSVDSSIPDLE
                     TGSVDRAKAGIGITYTVDFKTTEPLVGTGGVPVVKANNITLACAKNGGDTIFRCTRTV
                     GAEETDATVYGISTTLTDAAGNSRTVTIGSVMADKVLPSAVAGTAVLSIDAGADCGLT
                     QTQ"
     gene            complement(2247..3815)
                     /locus_tag="PKH10_07220"
     CDS             complement(2247..3815)
                     /locus_tag="PKH10_07220"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT27951.1"
                     /translation="MTKYFIILTALVLMIGCDNGDPKSPIDDTVVKDDTAVTDDVADS
                     EQPDEIADTDTATENTVVADDAEPDGKPDLDVPATCGNGVIDSGEICDGGSVYCGDLD
                     EKWAFGTTYCNDTCSGYDTYECQEPYADDDDTADPDEDEATDEEAEDDNVIPDSDEQI
                     DEDSVDIQPDPDMVDEDTYDDWVPDTADVQPDLDMVDEDLVDEDTVDVDIAPDADTVV
                     SGVLCTGQTKCYDNTAEMTCPTAGNDFYGQDSQYASLGCCTPRDYTVSGTTPNDIVTD
                     NVTGLIWQRTLPATYDGCTGESGTKCTWQEAIDYCDGLTYAGYTDWRLPSRKELATLP
                     EYGRDDPAIDTTIFPGTQSDWHWASSSSAGSTGSAWFVHFSLAVEVFQDKASTYYARC
                     VRGDTLSNSAFTEETVSGKIIVTDTVTGLIWTKEYSGTVTWENALSHCESLNYGGYTD
                     WRLPNIEELKTLLDDTIYSPASTFPEMPSSSFWSSSSLLFSNDNAWFVSFNNGAVSFD
                     PKTNINRHATRCVR"
BASE COUNT          863 a         1292 c         1378 g         1119 t
ORIGIN      
        1 cctgcgtctg tgtcagtccg cagtcggctc cggcgtctat cgagaggacc gccgtccccg
       61 ccacggcaga cggcaatacc ttgtcggcca tcaccgagcc gatggtgacg gtccggctgt
      121 tccccgccgc gtcggtgagc gtggtactta tcccatagac cgtcgcgtcg gtctcctcgg
      181 cccccacggt gcgggtacaa cggaatatcg tgtcgccgcc gttcttggca caggcgaggg
      241 ttatgttgtt cgccttgacg actggtacgc cgccggtgcc cactaacggc tcggtcgttt
      301 tgaagtcaac ggtgtaggtt atcccaatgc ctgccttggc gcggtcaacg cttccggtct
      361 ccagatcggg gatggaggag tccacagaga agtgggcatc gacagttccg ggatgtcccg
      421 ccttgtccga tccatcggcc aagatagtgt aggtgtcgtc gttgaatagc gatggactca
      481 tttcatacag ccacgagaaa ccggagacct gcgtaggcga tgaaagggtc attgatgctg
      541 cgggaatggt gacgactggt tctccggcaa gttcttccgt gctggtgaca ttaattctta
      601 tcgtgtcgtt cgcattaaaa gctgttttgt taaaatcgag ggtgagtacc ggtgcggtgt
      661 agtcgagacg gatgcccgct ttgagggggg cggcgtaggg gttgcccgct gcgtcctcga
      721 aggcaacggt gaggtcggcg atcccttcgg tgtgggtatc tttatctatg gtgagggcgc
      781 agtcgtagtg ttcggtgtcg gccccttggg tgcaggtgag ttgataggtg ccgagattca
      841 cgcggggggt ctctttgggc gcttcggtga gggtcagcgc tacggtgagg gtgtctttgt
      901 ccttgaggta gagggtggtc cccgcttcta cggcggtatc attcttttct atcgttgcgg
      961 tgaggtcggg gagcgttttg tcgatggtga gggtatcggt gagggggatg gtcgccgggt
     1021 tacccgctag gtcggtggcg atgacggtga aggtgtagtc cttttcgacg gtggcggggg
     1081 tcacttggta ttcgtagatg aagagttggg tgtcgttctg gtctccggtc cgcgtgaagg
     1141 tgaggtcgcc ggcatcgagc acgacgctct ggaccggttc atggaaactt atggcgatgg
     1201 ccactttgct cgtgccgttc gcggcggcgg gggagatgcc ttgattgacg atgatgggcg
     1261 ctttgttgtc gatgaatact ttccgggagg ccggcttggt ctcatagccc gctgcgtcgg
     1321 atgccttgat gaatacgatc cgctgggcgc ggtcgttggc cgggtcgggg ggatcgccga
     1381 gcgaccaatc gggatgggca tggtatccgg cgggacaggg ggatatgccg gtcgcttcgg
     1441 ggatcgtccc ggttgtcgct ccttcgaagc cggcgtcgtt ggcgatgtgt acggttgtcg
     1501 cgttcagcgc acggtagcag agggtcacgg tggtgtggcg cgttatcccc tcttcttcgg
     1561 tcagttcctt gccgtcgggg tcatagacct tcagggcggg agcggtcggt tttcccgttt
     1621 cggggtcaac gcccgccgcc ggggtcatga tgcaggcatt ctcgacgacg gtcactttac
     1681 cccgcgtcag tttgaatgcg ggggaggtgc agtagtaggc cacattgtcc tgtcgttcgg
     1741 cgggggcgat ctctttcttt tcgggggcat cggcggcgcg gatcgtgagt tcgagcgtca
     1801 tcgcgacacc gtaggggaga ttatcgaacc ggatctttgc atcgtcgcca cccagccacg
     1861 ccgggccggt cgtctttatc tcggtgccgt aggcgagttc tccccatgca tggaggagcg
     1921 tcgtatcggg cggggtgatg tcgttaccct ccgcgtcggt ccacgaatac ttcaggcgga
     1981 tgccgcccgc ctcgttcaga tcgcaggcgg tcagtagaaa gagcgccgca agcgctgtca
     2041 gtaagcactt tctcatggtg aattcacccc tcaggtgatc gatgttattt ccaataacga
     2101 tggattatac ttcagggaaa atacaaatca aaaaaaatag taggggaaga ggaagaccgg
     2161 tgaggggagt tgagcaggct ttgcgagttg agggagtaat ccggagccca cttccttaga
     2221 aagcgggcta aaaataaaaa ccacggtcat cgtacacatc gggtagcatg gcggttgatg
     2281 ttcgtcttag ggtcgaagct gacggcaccg ttgttgaaac taacaaacca tgcgttgtcg
     2341 ttggagaata gtaacgagga ggacgaccag aaagaactgg agggcatctc cgggaatgtg
     2401 ctggcggggc tgtagatggt gtcgtctagc agcgttttca attcttcgat attcggaagc
     2461 cgccagtctg tgtagccacc gtaattcagg ctctcgcagt gactaagtgc attttcccat
     2521 gttaccgttc cgctgtattc tttggtccag atgagtcccg tgacggtgtc ggtcacgata
     2581 attttacctg ataccgtctc ttcggtgaag gcgctgttag acagtgtgtc acccctcaca
     2641 caacgggcat aataggtact cgccttatct tggaagacct caacggcgag gctgaaatgc
     2701 acaaaccatg cgctgccggt actacccgcg ctagacgagg aggcccagtg ccaatccgat
     2761 tgcgtgccgg ggaatatggt ggtatctatc gccggatcat ctcttccata ttccggcaat
     2821 gttgccaatt ctttacggct cggcaatcgc cagtcggtgt aaccggcgta agtcagcccg
     2881 tcgcagtaat ctatcgcctc ctgccaagta catttcgtgc cgctctcgcc ggtgcatccg
     2941 tcgtaggtcg ccgggagggt cctctgccat ataagccccg tcacattgtc ggtgacaata
     3001 tcgtttggcg tagtgccgct cacggtgtaa tcgcgcggtg tgcaacaccc cagcgaggca
     3061 tattgtgaat cttggccgta gaagtcgttc cccgccgttg ggcaggtcat ctccgccgtg
     3121 ttgtcgtagc acttcgtctg tccagtgcag aggacgccgg agacaaccgt gtcggcatcg
     3181 ggcgcgatat ccacatccac cgtatcttcg tcaaccaagt cttcatcaac catatcaagg
     3241 tccggctgta catccgccgt gtcgggcacc cagtcatcgt aggtgtcctc gtccaccatg
     3301 tccgggtcag gctgaatatc aacgctatct tcatcgatct gctcgtcact gtcgggaatg
     3361 acattatcgt cttcggcctc ttcgtcggtt gcttcgtcct cgtcgggatc ggcggtgtcg
     3421 tcatcatcgg catagggttc ctgacattcg tatgtgtcat aaccggaaca ggtgtcattg
     3481 caataggtcg ttccaaaggc ccatttctca tcgagatcgc cgcagtaaac actaccgccg
     3541 tcgcagatct cgcccgaatc gatcaccccg ttaccgcagg tagcaggaac atcaagatcg
     3601 ggcttcccgt cgggctcggc atcatcggcg acaacggtgt tttcggtcgc ggtatcggta
     3661 tcggcgattt cgtccggttg ttcgctgtcc gccacatcgt cggtaacggc agtatcatcc
     3721 ttcaccacgg tgtcgtcaat cgggctcttg gggtcgccgt tatcgcaccc gatcatcagc
     3781 accagcgccg taaggattat gaagtatttg gtcatgggtc cccccttctg tggaaaagaa
     3841 cgaccaattt taccttttct tgagggagaa atcaaaatat attataggga ttttcctagc
     3901 ggggacatat cctgtccccg tatctccctc tccccggagc ccccgcctgc gaccgagggg
     3961 cggaaagagg ccatcagttg gctctactca aaggcgagga cctgtctgag gcaccgaagc
     4021 gccatcagcg cggaggtggc gagttccaca gcctcctctt gacgccccgg tccggccccg
     4081 ccggcggttg ctttggcggg cctaaaagcg gctttcttct ttcccctctt tcttctttac
     4141 cgccataaag aagaaagggg gtcggcgaag agcatcgcgc tcgccgaaac tctccccatc
     4201 cccgaagggc tacccacccc ccggcatacg caggacgccc ccctccgggg gcggttcggc
     4261 ctccggccaa cggcagcgac cagcggactc cgtagtacgc cgtcaggggg cagaatcccc
     4321 ctcttcttaa tgaatgattc cctcaacatt attaatctgt tccacattat atccacctca
     4381 acacctacaa tttcattacc ctatgggcaa catttatatt tacaaatact gcccacctgc
     4441 atataatgtc cccatgaaga acggagaccc cgccatctta accgccctct ctttcgtgag
     4501 caaactgctc aacgaggaag ggctcgctgt tctgcaaata tcctcctcgt ctcttcatat
     4561 tccttcctca aacaccacca tcaacttcct ctccttatca tacctgatcc cttccctgag
     4621 cgaggatatc ctcgcgctcc tccggcccca tc
//
LOCUS       DAOQUE010000204         4627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_20723_length_4627_cov_4.536308, whole genome shotgun sequence.
ACCESSION   DAOQUE010000204 DAOQUE010000000
VERSION     DAOQUE010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20723_length_4627_cov_4.536308"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..221
                     /gene="acpP"
                     /locus_tag="PKH10_07225"
     CDS             6..221
                     /gene="acpP"
                     /locus_tag="PKH10_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010019442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNT27952.1"
                     /translation="MINGADAAQPDAGKITETTSFIEDLNADSLDVVDFVMELEGAFS
                     IEIPDEDVEKIKTVGAAVEYISGKVNA"
     gene            258..1493
                     /gene="fabF"
                     /locus_tag="PKH10_07230"
     CDS             258..1493
                     /gene="fabF"
                     /locus_tag="PKH10_07230"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNT27953.1"
                     /translation="MRRVVITGVGIVSPVGNNATATWNGLIEGKSGIAPITQFDATNF
                     STQIAGEVKDFQPVAHGIDEKEAKRLDRYQHFCLATAIEAVRDSGIDFEKEDRERAGA
                     SIGIGFGGILSFENEHAKYMQNGPRRISPFIIPQMIANLGPGYVSIRFGLKGPQACPV
                     AACATGTFGVIEGYRLIKEGLADIMVSGGVEAAISPMSVGGFCSMKALSTRNDQPTKA
                     SRPFDAGRDGFVPAEGGAALLLEELEHAKARGAKIYAEVIGWGSSSDAYHVTQPAPGG
                     EGAFRAMKYALASAGLKPEQVQYINAHGTSTPFNDKLETAAIKQLFGDHAKKLKISST
                     KSMSGHLLGGAGSFEAAVTALSIHNQKIHGTMNYETPDPECDLDYVPNKALSLNITYA
                     MSNSFGFGGVNGVLVLGKY"
     gene            1511..2494
                     /locus_tag="PKH10_07235"
     CDS             1511..2494
                     /locus_tag="PKH10_07235"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNT27954.1"
                     /translation="MTTFSRILGFGHYLPAKVMTNADFEKMVETSDEWIRTRTGIRER
                     HFTEDGETASTMAAEAARRVIEKSGIDKNDIDAILVATVTADMHYPSTAALVQRTLGL
                     TRHIPAFDIGAACAGFVFGITTADSFIKSGLYRTILVIGTENLSKFTDMQDRNTCVLF
                     GDGAGATILQASDAPGILAAHLATDGNFSHLIEFPAGGSRIPATHETVEKRLHYTRME
                     GQEVYKQAVKDMSEAALESMKIAGLSLDQVSWIIPHQANTRIIETVGKRLNFPMEKVY
                     QNIDHVGNTSAASIPIALSEMEEKGLLKHGQNIVLTALGSGLTYGSVAFCY"
     gene            2659..3576
                     /gene="fabD"
                     /locus_tag="PKH10_07240"
     CDS             2659..3576
                     /gene="fabD"
                     /locus_tag="PKH10_07240"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNT27955.1"
                     /translation="MTKIVVFPGQGSQVVGMGKDLYENSSDARAIFDAADAALGFSIT
                     KICFEGPAEDLMLTKHTQPAILTVSYALWNELSKRHPIDGVKYMAGHSLGEYTALVAS
                     GAVDFAEAVRAVHQRGSFMQDAVEFGKGSMAAVIDVPRDTVYKICDESGHIVSPANFN
                     SPKQIVISGIAEGVDWVVNKLKTENKKAMKLKVSAPFHSELMKPAETRMKEVLWGMNL
                     NGFRTEVISNVTARPFTSIEEIRPLLTSQICGAVNWEDSILFAHRSGVTELIEVGPGN
                     VLAKLAGQIVPDMKTRNVANWTDIQNFGA"
     gene            complement(3596..3856)
                     /locus_tag="PKH10_07245"
     CDS             complement(3596..3856)
                     /locus_tag="PKH10_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27956.1"
                     /translation="MKKITFSLVVAILSFFLLSGCDLKGCLESCDENSDCLTQYNCYD
                     VSGRGKVCIPDECGGCFSSDQTCYYYQDPSDSTSCTFTYCGY"
     gene            complement(3955..>4627)
                     /gene="nth"
                     /locus_tag="PKH10_07250"
     CDS             complement(3955..>4627)
                     /gene="nth"
                     /locus_tag="PKH10_07250"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNT27957.1"
                     /translation="TMSRKSSLSPRDTVAILTRLRRWAASLEYAPVIDLMASQGDTPW
                     RILAATMLSARTKDETTAAAARRLFAIAENPEKTLTVPVAKLEKLVFPVGFYQVKAAR
                     LHELARAVIDRHGGKVPRTLDELTALPGIGIKTATLVQIKAFGIDEICVDTHVHRIAN
                     MWGIVNTKQPDDTRPALKKVVPKKHWRSVNQDLVTLGQTICKPQKHYCDRCPVSDLCP
                     VSTIR"
BASE COUNT         1005 a         1433 c         1338 g          851 t
ORIGIN      
        1 ctgcaatgat taacggcgcc gacgccgccc aacccgacgc cggcaagatc accgagacca
       61 cctcgttcat cgaagacctc aacgccgact cgctcgatgt cgtcgatttc gtcatggaac
      121 tcgaaggggc attcagcatc gagatcccgg acgaggatgt cgagaagatc aagaccgtcg
      181 gcgccgccgt ggaatacatc agcggcaaag tgaacgccta agaacggcac caccgcacaa
      241 gcataaggga ggcccggatg cgtagagtgg tcatcaccgg tgtcggtatc gtcagccccg
      301 tcggtaacaa cgccacggcg acctggaacg gactcatcga agggaaaagt ggcatcgcgc
      361 cgatcaccca gttcgatgcc accaatttct ccacccagat agcgggtgag gtgaaggatt
      421 tccagcccgt tgcccacggc atagacgaga aagaagcgaa acggctcgac cggtaccagc
      481 acttctgtct cgcgacggcg atcgaggcgg tccgcgacag cggtatcgac tttgagaaag
      541 aggaccgcga acgcgccggc gcctccatcg gcatcggctt cggcggcatc ctttcgttcg
      601 agaacgagca tgcgaaatac atgcagaacg gcccgcgccg catatcgccc ttcatcatcc
      661 cgcagatgat agcgaacctc ggccccggct atgtctcgat acgcttcgga ctcaagggac
      721 cgcaggcctg cccggtcgcc gcatgcgcca ccgggacctt cggcgtcatc gagggctatc
      781 gcctcatcaa ggaagggctc gctgacatca tggtgtcggg cggggtcgag gcggccatca
      841 gtccgatgtc ggtcggcggc ttctgctcga tgaaggcgct atcgacccgc aacgatcagc
      901 cgaccaaggc atcgcgtccc ttcgatgcgg gccgtgacgg cttcgtcccg gccgaaggag
      961 gcgcagcgct cctactggag gaactcgaac acgccaaggc gcgcggcgcg aagatctacg
     1021 ccgaggtgat cggttggggg tccagttccg acgcctacca tgtcacccaa cccgccccgg
     1081 gcggcgaagg ggcgttccgc gcgatgaagt acgcgctcgc ctcggccggc ctcaaaccgg
     1141 aacaggtgca gtatatcaac gcccacggca cttcgacccc cttcaacgac aaactcgaaa
     1201 cggcggcgat caagcaactt ttcggtgacc acgcaaagaa actcaagata agttcgacca
     1261 agtcgatgag cggccacctg ctcggcggcg ccggctcgtt cgaagcggcg gtcaccgcgc
     1321 tttccattca caaccagaag atccacggca ccatgaatta cgaaacgccc gaccccgagt
     1381 gcgatcttga ctatgtcccc aacaaggcgc tgtcgctcaa catcacttac gcgatgtcca
     1441 attccttcgg cttcggcggc gtgaacggcg tcctcgtgct cggcaaatac tgaagggaga
     1501 tcacccaatc atgacgacct tctctcgcat cctcgggttc ggccactatc tcccggcgaa
     1561 ggtgatgacc aacgccgact tcgagaagat ggtcgagacc tccgacgaat ggatccgcac
     1621 ccgcaccggc atccgcgagc gccatttcac cgaggatggc gagaccgctt ccaccatggc
     1681 ggccgaggcg gccagacggg tcatagaaaa aagcggtatc gacaagaacg acatcgacgc
     1741 catcctcgtc gcgaccgtca ccgccgacat gcactacccc tcgaccgcgg cgctcgtcca
     1801 gcggacgctc ggactgaccc gccacattcc cgccttcgac atcggtgccg cctgcgccgg
     1861 gttcgtgttc ggcatcacca ccgccgattc gttcatcaag agcggcctgt accggaccat
     1921 cctcgtcatc ggcaccgaga atctctccaa attcaccgac atgcaggatc gcaacacctg
     1981 cgtcctgttc ggcgacggcg cgggcgcgac gatacttcag gcgagcgatg ctcccggcat
     2041 cctcgcggcc catctcgcca ccgacggcaa tttcagccac ctcatagaat tccccgccgg
     2101 ggggtcgcgc ataccggcga cccatgaaac ggtcgaaaaa cgcctccatt acacccggat
     2161 ggagggacag gaggtctaca aacaggcggt caaggacatg tcggaagcgg cgctcgaatc
     2221 gatgaagatc gcagggcttt ctctcgatca ggtgagttgg atcatccccc atcaggcgaa
     2281 cacccgtatc atcgagacgg tcggcaaacg actcaacttc cccatggaga aggtctacca
     2341 gaacatcgat catgtcggca acacctcggc cgcgagcata ccgatagccc tctctgaaat
     2401 ggaggaaaag ggacttctca agcatggaca gaacatcgtg ctgaccgccc tcgggtcggg
     2461 actcacctac ggctcggtcg ccttttgcta ctagacccga atgtcatttt cttctgtaac
     2521 ataccgataa caaagagata tttattttct ttacctattg actttctttg cgtttttccg
     2581 tatcatcgca actagactgg cgtgtcagtc cagtatcgag accccggtgt ttgacaaaca
     2641 ggttttcgga gacgaaagat gaccaagatc gtggtgttcc cgggtcaggg ttcccaggtg
     2701 gtcggtatgg gcaaagacct ctatgagaac agcagcgatg cccgtgcgat attcgacgcg
     2761 gccgacgcgg cgctcggttt ttccatcacc aagatatgct tcgaaggccc cgccgaggac
     2821 cttatgctca ctaagcacac acagccggcg atactgaccg tgtcatacgc gctctggaac
     2881 gagctttcga aacgacaccc tatcgacggg gtgaaataca tggccggtca ctcgctgggg
     2941 gaatacacgg ctctcgtcgc ctccggcgcg gtcgacttcg ccgaagcggt ccgcgcggtc
     3001 catcagcgcg gttcgttcat gcaggatgcg gtcgagttcg ggaagggctc gatggcggcg
     3061 gtcatcgatg tcccgcgcga caccgtgtac aagatatgtg acgaatcagg ccatatcgta
     3121 agccccgcca acttcaactc gcccaaacag atcgtcatat cggggatcgc cgagggtgtc
     3181 gattgggtcg tcaacaaact caagaccgag aataagaagg cgatgaaact caaggtcagc
     3241 gcgccgttcc acagcgaact gatgaaaccg gccgagacgc ggatgaagga ggtgctttgg
     3301 gggatgaacc tgaacgggtt ccgcaccgag gtcatatcga atgtcaccgc acgccccttc
     3361 acttcgatcg aagagatccg cccgctcctc acctcacaga tctgcggcgc ggtcaattgg
     3421 gaggactcga tattgttcgc ccatcgcagc ggcgtcaccg aacttatcga ggtcggcccc
     3481 ggcaatgtat tggctaaact cgccggtcag atcgttcccg acatgaagac ccgtaatgtc
     3541 gccaactgga ccgatatcca gaatttcggc gcgtaatacc ggccagtccc gtctctcaat
     3601 acccgcaata agtgaaggtg caggaggtcg aatcgctcgg atcctggtag tagtaacagg
     3661 tctggtcgga actgaaacaa ccaccacact catcggggat acacactttt cccctgccgg
     3721 agacatcata gcagttgtac tgggtaagac agtcgctgtt ctcatcgcaa ctctcaagac
     3781 agcctttgag gtcacatccg gaaagcagga agaacgaaag gatcgctacg acgagggaaa
     3841 aggtgatctt tttcatggtc tcctcccgtg gtgatggtcc gatcggtcac cggtgcaccg
     3901 tccgagtgac ccgaagcgtc acaggataac cggaagcccg gtcgaaagca atttttaacg
     3961 gatcgtcgaa acggggcaga gatcgctgac cggacagcgg tcgcagtagt gtttctgcgg
     4021 tttgcagatg gtctggccga gcgtcacgag gtcctgattg accgaccgcc agtgcttttt
     4081 cggcaccacc ttcttgagcg cggggcgggt gtcgtcgggc tgcttggtgt tcacgatgcc
     4141 ccacatattc gcgatgcggt ggacatgggt gtcgacgcag atctcgtcga tgccgaaggc
     4201 ctttatctgg acgagcgtcg ccgtcttgat gccgatgccg gggagcgcgg tgagttcgtc
     4261 gagcgtccgg ggcactttcc cgccgtgccg atcgatcacg gcgcgggcga gttcatggag
     4321 ccgcgccgcc ttcacctgat aaaagccgac cgggaagacg agtttctcga gcttcgccac
     4381 cggcaccgtg agcgtctttt cgggattctc ggcgatggcg aagagtcgcc gcgccgccgc
     4441 cgcggtcgtc tcatccttgg tgcgggccga gagcatcgtc gcggcgagta tccgccacgg
     4501 cgtgtcgccc tgcgaggcca taagatcgat gacgggggcg tactcaagcg atgcggccca
     4561 ccggcggagc cgcgtgagga tcgccacagt gtcgcgcggc gaaagactac tttttctgga
     4621 cattgtc
//
LOCUS       DAOQUE010000205         4622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_20755_length_4622_cov_4.145172, whole genome shotgun sequence.
ACCESSION   DAOQUE010000205 DAOQUE010000000
VERSION     DAOQUE010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20755_length_4622_cov_4.145172"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="PKH10_07255"
     CDS             complement(<1..1668)
                     /locus_tag="PKH10_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT27958.1"
                     /translation="MDYSKTLNLPVTDFPMKANLPVKEQDILKKWEKIDIYRKVLDKN
                     RDRKSFVMHDGPPYANGHIHQGTVLNKILKDIVVKYMNMAGKHATFVPGWDCHGLPIE
                     LKAAERLGGKKDAMAPGDFRRACRAYAQEFIDIQRNEFKRLGVFADWEHPYITMTAGF
                     ESVIAHEFAKVIGSGSLLKAKKPVYWCMHCETALAEAEVEYHDHASPSIYVKFPVPAG
                     LPELPGEKVNFVIWTTTPWTLPANLAIALNPGFTYAAVKVRGEALIVADDLKDSFLKA
                     IGESGAPTLATFPADRFTGMAARHPFMDRDSVILYGDHVTLEAGTGCVHTAPGHGQED
                     YELGRKHGLDTYAPVKSDGSFTDEIPHWGGMKVTKANPQIVQYLFDHGFLLNKPGEMI
                     SHSYPCCWRCNSPIIFRATPQWFVSMDDTGLRAQALKNLEPVEFVPAWGKARISGMIE
                     NRPNWCVSRQRLWGSPIIAFHCRDCNEQIVDAGLAHKVADLFREHTSDIWYEKETAHF
                     LPKDFACPKCGSKNIGKETDILDVWFDSGVTWAAVLDGSKGLEFPVVF"
     gene            complement(1912..2274)
                     /locus_tag="PKH10_07260"
     CDS             complement(1912..2274)
                     /locus_tag="PKH10_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HNT27959.1"
                     /translation="MSELKALARENRKNPTKAEERIWYELLQNKQMLGHRFLRQKPIG
                     QFIADFYCAELKLVIEIDGQSHDYKLEHDEERTIELNKRGITVFRYTNDEVLQNLEAV
                     RADLERRITSLSVSLSKI"
     gene            complement(2506..3618)
                     /locus_tag="PKH10_07265"
     CDS             complement(2506..3618)
                     /locus_tag="PKH10_07265"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT27960.1"
                     /translation="MKASSKIKIGLVVAILLSVVFVKVYMIIQEKNEISTLIWKLQDP
                     LNDNFVLLRDIAEFGPAAVEAGPIILKSYDEDEKRKNDPFAGSFCKICAVRTLGYIGY
                     KEATEKLLGLLDDDQNWVMVYVTVESLGRLRAQEAIPALTNISRKHWFRTVRDAAVKA
                     LKYIDTGDSTIYPRSNNFPHEYFSFEDLEEVTARSSPIPEETKKLCRKAIEEKMTDPT
                     AIDRAKMDEILQKVTSVVDGNPNQRYYIDKTSKGIKVDGGYMIGTDHGEFGGHVLFVT
                     ERGEIELIARDHPIAIVTSPIFGIVVASGYRHLGQHFGVLFKVFKNDKNEWQTQRWFA
                     LPGAAVSMDVLKDGLYINAYGGDVVIRSDGSIMECD"
     gene            3929..>4622
                     /gene="mltG"
                     /locus_tag="PKH10_07270"
     CDS             3929..>4622
                     /gene="mltG"
                     /locus_tag="PKH10_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNT27961.1"
                     /translation="MRTILIVNVILLLIIGGYFLFKYRQYQEFQQTPIEIADPTTVTI
                     EKGMSWKGVAKKLFESGLIAEERYFYYLVKEKKWDKLLKAGEYEFQGSLLPEVVARTI
                     TEGKVKLYTVTIPEGFNKYDLAALLARLPWVRDGADFLKYCDAPRILRELGIPEATTC
                     EGLLFPSTYKFPRNVGVEEIIAVMDKRMQEVLAKYRDRMKQQGVKPYQVLALASVIEK
                     ESGLRDEQPRIAA"
BASE COUNT          921 a         1328 c         1265 g         1108 t
ORIGIN      
        1 gaacaccacc gggaactcga gccctttcga gccgtcgagc accgccgccc aggtcacccc
       61 cgagtcgaac cagacatcga ggatatcggt ctccttgccg atgttcttgg acccgcactt
      121 cggacaggca aagtccttcg gcaggaagtg ggccgtctcc ttctcgtacc agatatcgga
      181 ggtatgttcg cggaacaggt cggccacctt gtgggccagt cccgcgtcga cgatctgctc
      241 gttgcagtcc ctgcagtgga aagcgatgat cgggctaccc cagagccgct ggcgcgacac
      301 gcaccagttg gggcggttct cgatcatccc cgatatgcgc gccttccccc acgccgggac
      361 gaactcgacc ggctcgaggt tcttcagcgc ctgcgcccgc aggccggtgt cgtccatcga
      421 gacgaaccac tgcggcgtcg cgcggaatat gatggggctg ttgcaacgcc agcagcaggg
      481 gtaactgtgc gatatcatct cgcccggttt gttgagaagg aacccgtggt cgaagaggta
      541 ttgcacgatc tgcgggttgg ctttggtcac cttcatcccg ccccagtgcg gtatctcgtc
      601 ggtgaaactg ccgtcgcttt tgaccggcgc ataggtgtcg agcccgtgtt tccgccccag
      661 ttcgtagtcc tcctgcccgt gacccggcgc ggtgtggaca cagccggtgc ccgcctcgag
      721 cgtgacatgg tcgccgtaga gtatgaccga atcgcggtcc atgaaggggt ggcgcgccgc
      781 catcccggtg aagcggtcgg ccgggaaggt ggcgagcgtc ggcgcacccg actctcctat
      841 cgccttgagg aaactatcct tcaggtcgtc ggcgacgatg agtgcttcgc cgcggacctt
      901 caccgccgcg taggtgaacc cgggattgag cgcgatggca aggttcgccg gcagggtcca
      961 cggcgtcgtg gtccatatca cgaagttgac cttctccccc ggcagttcgg gcagtcctgc
     1021 cggcacgggg aacttcacat agatggaggg ggatgcgtga tcgtggtact ccacctccgc
     1081 ctcggcgagc gccgtttcgc agtgcatgca ccagtagacc ggttttttcg ccttgaggag
     1141 cgaccccgac ccgatcacct tcgcgaattc gtgcgcgatg accgactcga acccggcggt
     1201 catcgtgatg taggggtgct cccagtcggc gaagaccccc agtcgcttga attcgttacg
     1261 ctggatgtcg atgaactcct gcgcgtaggc gcggcaggcg cggcggaaat cacccggcgc
     1321 catcgcgtcc tttttgccgc cgagtcgctc ggccgccttg agttctatcg gcagaccgtg
     1381 gcagtcccag ccggggacga aggtggcgtg tttccccgcc atattcatgt acttgacgac
     1441 gatatccttg agtattttgt tgagcaccgt tccctgatgg atgtggccat tcgcgtacgg
     1501 cggaccatcg tgcatcacga aacttttcct gtcgcggttc ttatcgagaa ccttccggta
     1561 aatatcgatc ttctcccatt ttttgaggat atcctgttcc ttcaccggca gattggcctt
     1621 catcgggaaa tcggtcaccg ggagattgag tgtcttactg tagtccattc gtaggtctcc
     1681 atatccgcta taaaaagcag gcgggattgt agcaggcggg cgggaagtgt caagtaaaca
     1741 ccctcagtca acaccctcag tcggctttgc cgacagctcc ctgcgacagg gagccggttc
     1801 cggttaggcc ccctgatgca gggggctccg cgaagcggtg ggggtgttgg ttcaaggcac
     1861 accctccgtc acttcgtgac acctccctgc gacagggagg ccatgcggat ttcatatttt
     1921 cgacagggag actgaaaggg aggtgatgcg gcgttcgagg tcggcgcgga ccgcttcaag
     1981 gttttgcagg acttcgtcgt tggtgtagcg aaagaccgtg atgccgcgct tgttgagttc
     2041 gatggtgcgt tcttcgtcgt gctccaactt gtagtcatgg ctctggccgt cgatctcgat
     2101 caccagtttc agttcggcgc agtagaaatc ggcgatgaat tggccgatgg gtttttgtct
     2161 gaggaagcga tgaccaagca tttgtttgtt ctggaggagt tcgtaccata tccgttcttc
     2221 ggccttcgtc gggtttttgc ggttctcgcg ggccagtgct ttcagttcgg acatgaagcg
     2281 gaacattatc tcacaccctc aatcgtcctt cgccgacagc tccctgcgac agggagcctg
     2341 tgcggtttgg ccaacacaca ccctcaggcc gttggccagc tccctgtgac agggagccag
     2401 ttcggtttgg ccccctgata cagggggctt gcgggggtgt tttggtccaa cgcacacccc
     2461 ccgtcactgc atgacacctc cctgcgacag ggaggcaacc attctttagt cgcattccat
     2521 aatcgaacca tcgctgcgta taaccacatc cccgccgtaa gcattgatat agagcccatc
     2581 ttttaacaca tccatagaaa cggccgctcc gggaagagca aaccaccttt gagtctgcca
     2641 ctcatttttg tcatttttga acaccttaaa caaaacgcca aaatgttgcc ccaagtgtcg
     2701 ataaccggaa gccaccacta tgccgaatat tggtgaagtt acaatggcga ttgggtgatc
     2761 ccttgcaatc aattcaattt cgcccctttc cgtgacgaat agcacatgtc cgccaaattc
     2821 gccatgatcc gttccgatca tatatccgcc atcaactttg attcccttgc ttgttttatc
     2881 aatgtaatat cgttgatttg gattgccgtc aacgacagag gtaacttttt gcaaaatctc
     2941 gtccattttt gccctatcga tggccgtggg atctgtcatt ttttcttcga ttgccttacg
     3001 acaaagtttt ttggtctcct ccggtattgg cgatgacctt gccgttactt cctctagatc
     3061 ttcgaaagag aaatactcgt gtggaaaatt attggatctc gggtagatgg ttgaatctcc
     3121 ggtatcaata tatttcaatg cttttacagc cgcgtccctg accgttctga accagtgttt
     3181 ccgggaaata tttgtaagtg ctgggatggc ctcttgtgcc cgtaatcttc ctagactttc
     3241 aaccgtcaca tataccatga cccaattctg atcatcatca agaaggccta acaatttctc
     3301 ggtcgcttct ttatagccaa tatatcccag tgtccttacc gcacatattt tgcaaaaaga
     3361 acccgcgaat gggtcattct ttcttttctc atcttcatcg taactcttga gaatgatcgg
     3421 gccggcttcg actgctgcgg gtccgaattc ggcaatatcc ctcaacaaca caaagttgtc
     3481 attcaagggg tcttgcagtt tccatatgag ggttgatatc tcatttttct cttgaatgat
     3541 catatacact ttaacgaaaa caacagaaag aagaatggcg accacaagtc ctattttgat
     3601 ttttgaactc gccttcatgg ttctcccccg ccagaaataa cttgttcatt ctaccgcatg
     3661 gaagaatcat tgcaagaaag aatgtaagtg ttgccgggat gagcgtgttt acctccctgt
     3721 cgcagggagg tggcgcgaag cgccggtggg tgtgggtcgc acaacacccc tcctgcctcc
     3781 cctgcatcag gggagcatcc ttgcaggccc cctgtcgcag ggggctccgc ggagcggtgg
     3841 gggtgttttt cggcctccct gttttctttg catcctcttt ttttttctta taatgcgtcc
     3901 cgtaccatga gcgggcggag gcgagaccat gcggaccatc cttatcgtca atgtcatcct
     3961 gttgcttatc atcggcggct atttcctgtt caagtaccgg cagtatcagg agttccagca
     4021 gaccccgatc gagatcgccg atccgaccac cgtgaccatc gaaaagggga tgtcatggaa
     4081 gggggtcgcc aaaaaactct ttgagagcgg gctcatcgcc gaggagcgtt atttctatta
     4141 cctcgtcaag gagaaaaagt gggataaact gctcaaggcc ggcgagtatg agttccaagg
     4201 gtcgctcctg cccgaggttg tcgcccgcac catcaccgaa ggcaaggtca aactctacac
     4261 tgtcaccatc cctgaagggt tcaataagta cgacctcgcg gcgctccttg cgcggctccc
     4321 gtgggtccgc gacggcgccg atttcctgaa atactgcgac gcgccgcgca tcctgcgcga
     4381 actcggcatc cccgaggcga cgacctgcga ggggctcctg ttcccctcga cctacaaatt
     4441 cccgcgcaat gtcggcgtcg aagagatcat cgcggtcatg gacaaacgga tgcaggaggt
     4501 gctcgcgaag taccgcgacc ggatgaaaca gcagggggtc aagccctatc aggtgcttgc
     4561 gctcgcctcg gtcatcgaga aggaatcggg gctccgcgac gaacagccgc gcatcgccgc
     4621 cg
//
LOCUS       DAOQUE010000206         4614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_20789_length_4614_cov_4.661110, whole genome shotgun sequence.
ACCESSION   DAOQUE010000206 DAOQUE010000000
VERSION     DAOQUE010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20789_length_4614_cov_4.661110"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PKH10_07275"
     CDS             complement(<1..897)
                     /locus_tag="PKH10_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNT27962.1"
                     /translation="MPRSLVFFLLMALMVASGPLSAAKKKFTPDEGSEVKQEEAKETK
                     EAKEPTADKKGKAVKKKKKTASEAQPTAPEPELKPVRLPPAEKKAPVTEPASAPAPAP
                     APAPAPAPVPAPAPVPAPAPVPAPAPAPAPAPAPAPAPAPAPTPAPAAKSDQATKGDP
                     VVLSGVVLDADTKKAVIGAKITTNAFPYPALTAKEGSFKYPMKLKAGTYTLEVAAPGY
                     KTLSLSLEVVTGMKKLPMTIWLALDSSPYRSLAEHPDGKPFEPVGVRGAEKALAARSA
                     NVDPSGEGDTPKPGIHLITGLLK"
     gene            1112..1552
                     /locus_tag="PKH10_07280"
     CDS             1112..1552
                     /locus_tag="PKH10_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27963.1"
                     /translation="MSHNYLCPRCRVALYDQASNKIVLTGKLRGIHFEVTGTFELNHQ
                     KDEFGGTIRDCPQVSVKKGARVDFFCPHCGFDLSPAYDQELTELIHVDETGGEKAFFI
                     SKISGKEMSFLISKETKEVIRSYGKDKDDYMNRFVEYFHLWNKF"
     gene            complement(1568..1795)
                     /locus_tag="PKH10_07285"
     CDS             complement(1568..1795)
                     /locus_tag="PKH10_07285"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HNT27964.1"
                     /translation="MQQYKVVPAQEIEKRLHRTVKEHETLGEQVDELSRKEVITPEDE
                     MELKRLRKLKLYKKDMIAYLKSMLQGGAKPE"
     gene            complement(1812..2531)
                     /locus_tag="PKH10_07290"
     CDS             complement(1812..2531)
                     /locus_tag="PKH10_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27965.1"
                     /translation="MRGSVLVWDNSSGEAFFQWCSDDRVVARVERYERRRFLTDFPPV
                     LEQIVATTPVDADTVVYFGAGPGSFTALKTGAAMLGGLLYARGVTRVRLISSLDILSL
                     RTTVPCGAPCVALIPYRGDEVFLSVAVESGTGKRCYPIHPRAVPFAELDAILDRFAGM
                     GGVVIAGGDLPVAVERSLSARSGTLTRRYPSPELSVERLDGVPRLGTVEIGSEPLMLR
                     YMVAPADLPAGDREEYYVASN"
     gene            complement(2534..4144)
                     /gene="rseP"
                     /locus_tag="PKH10_07295"
     CDS             complement(2534..4144)
                     /gene="rseP"
                     /locus_tag="PKH10_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNT27966.1"
                     /translation="MLKFLLALVLFGLVIFVHELGHFLAARFFRVKIFVFSLGFGSAL
                     VSWRRGETEYRLSAVPLGGYVKMLGENTAEEEIPPEDLPRSFSAKEWWQKVIIVFAGP
                     LTNIVFAVLIYFVVSFFNHTSDATLVEFISPGGPAAQTALREGDRVLAINGRRTAVWE
                     DLQNALPLPVAGACPPIEVLVARWPSGDTETVPVSPEKRRYADVFGEESDRCVMGIAA
                     LPRDTRVAFAGPIEGLKNGDIVRSVDGRTVTRWYEMRTLLTADSAHHLIVYRDGKEEA
                     VSLTAEEATQVAALIRHGGMLVGEVEPDSVAQKAGVENGDLIVAVNGVTLAAPHEFVS
                     EVKKVKEGGSVRLSLYRGGESMEKEFVLSFDSKENQYTGMTESSIRWGARFLFSYDLE
                     PTQARREAPLLYSARYAVEETGNIAVMTVKGMYYLLTNKLPAKAIGGPILVFDISQKA
                     AARGKKVFLSVMAMISVNLGILNLLPVPVLDGGHAVMYTWEGLTRRKIPLRVKEWSLR
                     AGVALLMALMIFAMFNDISRYISIFSGG"
     gene            4296..>4614
                     /locus_tag="PKH10_07300"
     CDS             4296..>4614
                     /locus_tag="PKH10_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNT27967.1"
                     /translation="MSYQVIARKWRPARFDEVVGQDQVTGTLRYATKEGRVAPVVLFT
                     GIRGTGKTTLARILVKALNCADPQADGEPCNRCESCREITAGRSPDVLEIDGASNNSV
                     DDVR"
BASE COUNT          972 a         1378 c         1347 g          917 t
ORIGIN      
        1 cttcaggagg ccggtgatca aatggatacc cggcttcgga gtatcccctt cccccgaagg
       61 atcaacgttg gcggatcggg cggcgagcgc cttttccgca ccacgcacgc ccaccggctc
      121 gaacggttta ccatcggggt gttccgcgag agatcgatac ggggaggaat cgagagctag
      181 ccatatcgtc ataggcaatt tcttcatccc ggtcacgacc tcgagagaaa gtgagagcgt
      241 tttatatccg ggtgccgcga cctccagcgt ataggtaccc gctttcagtt tcatgggata
      301 tttgaaggat ccctctttcg ccgtcagcgc cgggtaagga aaggcgttgg ttgttatttt
      361 cgcgccgatg accgcttttt tggtgtcggc gtcgagaacg acacccgaaa ggaccaccgg
      421 atcacctttc gtggcctgat cagacttagc ggccggagcc ggagtcgggg caggggcagg
      481 ggcaggggcc ggggcagggg ccggggccgg ggccggagcc ggagcaggga ccggagccgg
      541 agcagggacc ggagccggag cagggaccgg agccggagca ggggccggag caggggccgg
      601 agcaggggcg gaagcaggtt ctgttacagg cgcctttttc tccgcaggcg gcaaccttac
      661 cggtttcagt tccggttccg gagcggtcgg ttgcgcctct gaagcggtct tcttcttttt
      721 ctttacggcc tttccctttt tgtcggcggt cggctctttt gcctccttgg tctctttcgc
      781 ctcttcctgt ttgacctcgc ttccctcgtc gggcgtgaac tttttcttgg cggcggagag
      841 gggaccgctg gcgaccatca aggccataag gagaaaaaac actaatgaac gcggcatact
      901 atcctccgta cccgatatta cgatacctga tgtccttttt tgcgcggtac cataatcaat
      961 ggacagggtc tggtataaca cttcttgaga ctcatgtcaa aaaaataaag gaacgggatg
     1021 ctttgttccc gtcagggatt tttcttgctt tgagggaggt taatccatac catgtccgtg
     1081 agttgaactt ttgggagaga ggaggttctt catgtctcac aattatctgt gcccccggtg
     1141 ccgcgtcgcg ctgtatgatc aggcatcgaa caagatcgtg ctgaccggca aactgcgagg
     1201 tatccatttc gaggtgaccg gcaccttcga attgaatcat cagaaggacg agttcggggg
     1261 aacgatccgc gattgcccgc aggtctcggt caaaaaaggc gcccgtgtcg attttttctg
     1321 ccctcactgt ggattcgatc tttcccctgc ctacgatcag gaactgaccg aactcatcca
     1381 tgtggatgag accggcggag aaaaggcttt cttcatcagc aagatatcag gaaaggagat
     1441 gtccttcctc atctcgaagg agaccaagga agtgatccgt tcatacggca aggacaagga
     1501 cgactacatg aatcgattcg tggagtattt ccacctgtgg aacaagttct gacctgttga
     1561 cgcgccgtta ttccggtttt gcgccgcctt gtagcatgct tttcaggtag gcgatcatat
     1621 cttttttgta caatttcagt ttgcggagtc gcttcaattc catttcgtcc tccggggtga
     1681 tgacctcctt gcgggagagt tcgtccacct gttcgccgag cgtctcatgc tccttcaccg
     1741 tccgatgaag ccgcttttcg atctcctgcg cggggacgac cttgtactgc tgcatggcac
     1801 acctccttgc gtcagttact tgcgacataa tattcctcac ggtcacccgc cggcagatcg
     1861 gccggggcga ccatataccg aagcatgagc ggctcggaac cgatctcgac cgtcccgagg
     1921 cgaggcaccc cgtcaagccg ttccacggac agttccggcg acggatagcg acgggtcaag
     1981 gttccggatc gcgcggaaag ggatctctcg acggcgacgg gcagatcgcc accggcgatc
     2041 acgacccctc ccatacctgc aaaacgatcg agaatggcat ccagttctgc gaacggcacc
     2101 gcccggggat gtatcgggta acatcgcttg ccggtaccgc tttccacagc gacggaaagg
     2161 aacacctcgt cgccacggta cgggatgagc gccacgcagg gcgcaccgca cggaacggtc
     2221 gttcgaagtg aaaggatgtc cagcgaagag atcaagcgca ccctcgtcac gccccgggcg
     2281 tagaggagcc ctcccaacat cgccgctccg gtcttgagcg cggtgaagga gccgggaccg
     2341 gcgccgaaat agaccacagt gtcggcatcg accggtgtcg tcgcaacgat ctgttcgagc
     2401 acgggcggga aatcggtcag gaaccttcgc cgttcatatc gttcgacccg cgcgaccacc
     2461 cgatcgtccg aacaccactg aaaaaaggcc tcgcccgacg agttgtccca gaccagcacc
     2521 gatccccgca tccctagccg ccgctgaata tagatatgta gcgcgatatg tcgttgaaca
     2581 tagcgaagat catgagcgcc atcaagaggg cgacgcccgc gcggagcgac cattccttca
     2641 cgcggagcgg tatcttccgt cgcgtcagac cttcccaggt gtacatgacg gcgtgaccgc
     2701 catcaaggac cggcaccggc aacagattga gaatgccgag gttgacgctt atcatcgcca
     2761 tgaccgagag aaagaccttc ttgccccgcg ccgccgcctt ctgcgatatg tcgaacacca
     2821 gtatcggccc gccgatcgcc ttggcgggaa gtttattggt caggagatag tacatccctt
     2881 taacggtcat caccgcgatg ttgccggtct cttccacggc atagcgcgcc gaatagagaa
     2941 gcggcgcctc cctccgggcc tgcgttggct caaggtcata tgagaaaagg aaacgggcgc
     3001 cccaccggat gctgctttcg gtcatgccgg tatactggtt ctcctttgag tcgaaggaaa
     3061 ggacgaactc cttttccatg ctttccccgc cgcggtagag cgacaatctt accgaaccac
     3121 cctctttgac cttcttcacc tcgctgacga actcgtgggg cgcggcgagt gtcaccccat
     3181 tcacggcgac gatgagatca ccgttctcga cgcccgcctt ctgggcgacc gaatcgggtt
     3241 cgacctcgcc aacgagcatc ccgccgtgcc gtatcagcgc ggccacctgc gttgcctcct
     3301 cggcggttaa agataccgct tcctcctttc cgtcacgata gacgatcagg tgatgggcgc
     3361 tgtcggcggt cagaagtgtg cgcatctcat accaacgggt caccgtcctc ccgtcgaccg
     3421 atcgcacgat gtcgccgttc ttcaagccct cgatcggccc agcgaacgcc acgcgggtat
     3481 cgcgcggcaa ggccgctatc cccatcacgc atcgatcgct ctcttcgccg aacacatcgg
     3541 catatcgccg tttttcggga gagaccggca ccgtctcagt atctcccgac ggccagcggg
     3601 cgaccagcac ctcgatagga ggacacgcac cggcgaccgg tagaggaagc gcgttctgca
     3661 ggtcctccca taccgccgtc cgccgtccgt tgatggcgag gacgcggtcc ccttcccgca
     3721 atgcggtctg agcggcgggc ccaccggggg agatgaactc gaccagggtc gcgtcgctgg
     3781 tgtgattgaa aaaggaaacg acgaagtaga taagcaccgc gaagacgatg ttcgtcaacg
     3841 ggccggcaaa gacgatgatc accttctgcc accactcctt cgccgagaaa ctgcgcggca
     3901 ggtcctcggg agggatctcc tcctcggcgg tattctcgcc gagcatcttg acatagccgc
     3961 cgagcgggac cgccgaaagc cggtattcgg tctcgccgcg acgccaactg accagcgccg
     4021 agccgaagcc gagcgaaaag acgaagatct tgaccctgaa gaaccgcgcc gcaaggaagt
     4081 gtcccagttc atggacgaag atgacgagtc cgaacagaac gagcgccaga aggaacttga
     4141 gcataactct ccgatatccg attacctgaa gtacagtata gcgacaaccg gagggaagga
     4201 aagattaccg gccaaaaaag ttatcgaaag agtttgcaaa accgctcatt tgtggataat
     4261 gacctgacga aactggaaag aaaggggaac tgtccatgag ttatcaggtc atcgcgcgga
     4321 aatggcgtcc cgcccggttc gacgaggtgg tagggcagga tcaggtgacc ggcaccctgc
     4381 ggtacgctac caaagagggg cgcgtcgcac cggtcgtcct gttcaccggt atccgcggca
     4441 ccggcaagac aacgctcgcc cgcatcctcg tcaaagcgct caactgcgcc gatccccagg
     4501 ccgatggcga accgtgcaac cgctgcgaga gttgccgcga gataacagca ggacgtagcc
     4561 ctgatgtgct cgagatagac ggcgcgtcga acaactcggt ggacgatgtc cgca
//
LOCUS       DAOQUE010000207         4594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_20905_length_4594_cov_4.061467, whole genome shotgun sequence.
ACCESSION   DAOQUE010000207 DAOQUE010000000
VERSION     DAOQUE010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20905_length_4594_cov_4.061467"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PKH10_07305"
     CDS             complement(<1..650)
                     /locus_tag="PKH10_07305"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNT27968.1"
                     /translation="MWELIESNKRRSLWLFFALFLVLMVLGGLIGASFIEPYGAWWGV
                     GLAAALSLILGAVAYWAGDDIVMSVGRATLVGKESSPQLYNIVEEMQIAAGFGRMPEV
                     YIVADDSMNAFATGIRPEKSAIAVTVGLLENLSRDELQGVVAHEMSHIANRDVLFMTF
                     SGVLLGSITMLSEGFLRGFAFTGGDSRRSKLESSSRSHPTIMIAALMFALLAPLLAR"
     gene            complement(682..1254)
                     /locus_tag="PKH10_07310"
     CDS             complement(682..1254)
                     /locus_tag="PKH10_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNT27969.1"
                     /translation="MWSILVFLALLVAVVVGVFLFGVAIYNGLVGLRNRVKNGWSQID
                     VQLKRRHDLIPNLVETAKGYMQHERETFIRVTEARAKAMGAGAGSPREVGQAKSGLSD
                     ALAKFLLTVESYPELKANENFLRLQEELTSTENRIAYARQAYNDDVLMLNNKIEMFPS
                     NIVAGMFQFHKAEFFQIEDVTERKAPQVKF"
     gene            complement(1259..1657)
                     /locus_tag="PKH10_07315"
     CDS             complement(1259..1657)
                     /locus_tag="PKH10_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNT27970.1"
                     /translation="MKKDMVVLELDRVEVDHCLVCGGLWLDAGELELLAGTPDKATAL
                     AILFRDLSDTAGETARPCPICGAKMMKQATATEPPLVIDRCPAGHGVWFDDGELHALF
                     SCLKCGAENRVFSLLQDMFAARIQTSVKGG"
     gene            complement(1709..2296)
                     /locus_tag="PKH10_07320"
     CDS             complement(1709..2296)
                     /locus_tag="PKH10_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNT27971.1"
                     /translation="MVFINKVAKEVNVKIVYYGAGLSGKTTNIEYIYNKVDPNKRGKL
                     ISLKTETERTLFFDFMPVDIGKVGDMSVKVHLYSVPGQVFYDHSRKLILKGTDGIVFV
                     VDAQVERMDANVESFSNLKKNLLMNSILFEKIPLVIQYNKVDLPNAAPVPDMQKLFNP
                     QGRYPELIASALSGEKVFDTFKAIVRMVIRTIPLS"
     gene            complement(2296..2772)
                     /locus_tag="PKH10_07325"
     CDS             complement(2296..2772)
                     /locus_tag="PKH10_07325"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNT27972.1"
                     /translation="MQVTEQIAAFLAEKKKALSLSDMIVLESTNGFILASTATGEAMD
                     LESLGSLTSGGLTAFDMLSTLFAAGQISYLLMETAEKNFLLQRVEENYFLLAVAPKQV
                     RAGYLRLRVEKILPDLAALVRRFCEENAVSIGDIDIDEITASLDAQFDELLKGGDR"
     gene            complement(2871..3887)
                     /locus_tag="PKH10_07330"
     CDS             complement(2871..3887)
                     /locus_tag="PKH10_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27973.1"
                     /translation="MKVSRLFFAVACVILLAACGDNATNTVPDNAATDNAVPDETVND
                     EIAVDDIPVIDETPDEDEAEDTAPVEFDVCCGDEATDDMSDDLVDEDTEQPDEGHPLL
                     DPFVGTWAEKLVLKSNTHTGIGDVPSTTTRYKLGHISIENGELKIEQKICKIDNTTVD
                     PISGEGHTIFYQPFCDIYWYWRPGELSNPKPDITVTDNGGTITFVENRSWELRGMHKM
                     DNVETDLMPTAADDARIFDHDADDKPAFTIGFTSSLIKGDIYFTQRLSHELTGTVVSP
                     GRIEGSVNWTDSQYTVDSTNPTLKGQKESTILNDQSTFIYVKVADAFTCEEVYGQKDT
                     LFAN"
     gene            complement(3908..>4594)
                     /locus_tag="PKH10_07335"
     CDS             complement(3908..>4594)
                     /locus_tag="PKH10_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27974.1"
                     /translation="LQSDNGQIELQNKTQVWGWDYEDDDQRYLGSAGSMTYGYEPLRV
                     GGGISYAFDIGHDYKLAPVLGGMWHHWSTYENRHGTKPTDKWRETASVNAGLHLKHEE
                     RQVGFDFQFVQSPVPEQTGRETYVDNHRLIWAAGWTEYFEFEKVTVAGGVQAQMQWLL
                     YHTTMKDLSRGEDAGGIVDEFPDDANNYMDPDVPFASAQGLQTNNPGFPGFRSGGFLV
                     GTSLFVKILY"
BASE COUNT          871 a         1384 c         1357 g          982 t
ORIGIN      
        1 cgggcgagaa gcggcgcaag cagggcgaac atcagcgcgg cgatcatgat cgtcgggtgc
       61 gatcgtgaag aggactcgag tttactgcgg cgcgaatcgc cgccggtgaa ggcgaatccg
      121 cgcaggaacc cctcgctcag catcgtgatg gacccgagca ggacccccga aaaggtcatg
      181 aaaaggacat cgcgattggc gatatgggac atctcgtgcg ccacgacccc ctgtagttcg
      241 tcgcgggaaa gattctcgag aagcccgacg gtcaccgcga tcgccgattt ctcggggcgg
      301 ataccggtcg cgaaggcgtt catcgagtcg tcggcgacga tgtacacctc gggcatccgg
      361 ccgaaaccgg ccgctatctg catctcctcg acgatattgt acagttgggg actcgattcc
      421 tttccgacga gcgtcgcgcg accgaccgac atcacgatgt cgtcgccggc ccagtaggcg
      481 accgcgccga gtatcaggga gagcgccgcg gccagaccga caccccacca cgcgccgtac
      541 ggctcgatga acgacgcgcc tatcagcccg ccgagcacca tgagaacgag gaacagcgcg
      601 aaaaagagcc acagcgatcg acgcttgtta ctttctatga gttcccacac cgccggtatc
      661 ccccgtcgag gcgcgcggcc gtcagaactt cacctgcggc gccttccgct cggtgacatc
      721 ctctatctgg aagaattcgg ccttatggaa ctggaacatc cccgcgacga tgttcgacgg
      781 gaacatctct atcttgttgt tgagcatgag cacatcatcg ttataggcct gtctcgcata
      841 ggcgatgcgg ttctcggtcg aggtgagttc ctcctgcagg cgcaggaagt tctcgttcgc
      901 cttgagttcg ggataacttt cgaccgtgag caggaacttg gcaagcgcgt cggaaagccc
      961 cgatttggcc tgccccactt cgcgcggaga accggcaccg gcgcccatcg ccttggcgcg
     1021 cgcctcggtg acgcggatga aggtctcgcg ttcgtgctgc atatagccct tcgccgtctc
     1081 cacgagattg gggatgagat cgtgccgtcg cttcaactgc acatcgatct gcgaccaccc
     1141 gttcttcacg cggttcctga gccccacgag accgttgtag atggcgacgc cgaaaagaaa
     1201 gacgccgacg acgaccgcca cgagaagagc gaggaacaca aggatcgacc acaccatgtc
     1261 agcctccttt tactgaggtt tgtatccgtg ccgcgaacat gtcctgtaga agcgagaaca
     1321 cccggttctc cgcgccgcac ttaagacagg agaagagcgc atgcaactct ccgtcgtcga
     1381 accagacgcc gtgaccggcg gggcaccggt ctataacaag cggcggctcc gtggcggtcg
     1441 cctgtttcat catcttcgcc ccgcagatcg ggcaggggcg cgccgtttcc ccggcggtat
     1501 cggaaaggtc acggaacagt atcgcgagcg ccgttgcctt atcgggggtc cccgccagca
     1561 gttccagttc accggcgtcg agccacagcc ctccgcagac aagacagtgg tccacctcga
     1621 cgcgatcgag ttcaagcacc accatatctt ttttcacgca taccgggcac cggagcatcg
     1681 atcggtacct cgccgttcct gttaggacct atgacagggg aatggtgcgt atcaccattc
     1741 tcacgatcgc cttgaatgta tcgaatacct tctcccccga gagggccgag gcgatgagtt
     1801 ccgggtacct gccctgcggg ttgaaaagtt tctgcatatc cgggaccggc gccgcgttgg
     1861 gcagatcgac cttgttgtac tgtatcacga gcggtatctt ctcgaacagt atcgagttca
     1921 tgagcaggtt cttcttgagg ttggagaaac tctccacatt ggcgtccatg cgctccacct
     1981 gcgcgtcgac gacgaacacg atgccgtcgg tccccttgag gatgagtttg cgcgaatggt
     2041 cgtagaacac ctgtccgggc accgagtaga ggtgcacctt gacgctcatg tcgcccacct
     2101 tgccgatgtc gacaggcatg aagtcgaaaa agagcgtccg ctcggtctcg gtcttgagcg
     2161 atatgagttt cccgcgcttg ttcggatcga ccttgttgta gatgtattcg atgttggtgg
     2221 tcttgcccga caggccggca ccgtagtaga cgatcttgac attgacctct ttggcgacct
     2281 tgttgatgaa caccattacc ggtcgccccc tttgagcaac tcatcgaact gggcgtccag
     2341 cgaggccgtt atctcgtcta tgtcgatgtc gccgatgctc accgcgttct cttcgcagaa
     2401 ccggcggacc agcgcggcga ggtcgggcag tatcttttcg acccgcaggc ggagatagcc
     2461 ggcgcgcacc tgtttgggcg ccaccgcgag caggaaatag ttctcctcca cgcgctggag
     2521 cagaaaattc ttttcggcgg tctccatgag caggtaggat atctggccgg cggcgaagag
     2581 cgtcgagagc atgtcgaacg ccgtcaggcc gccgctcgtg agcgagccga gcgactcgag
     2641 atccatcgcc tcgcccgtcg cggtggaggc gaggatgaac ccgttagtgc tctccagcac
     2701 gatcatgtcc gaaagcgaga gcgccttttt cttttcggct aggaacgccg ctatctgttc
     2761 agtcacctgc atgccggcct caacggttga cgatggggga gcagggcatg gtagcgtatc
     2821 ggatggtttt tttcaatttt tttgccgatt ctttgcggcc cggcgaccgg tcagttcgcg
     2881 aacagcgtgt ccttctggcc gtagacctct tcgcaggtga aagcatccgc cactttcaca
     2941 tagatgaagg tgctctgatc gttgaggatc gtgctctcct tttgtccttt aagggttgga
     3001 ttggtcgagt cgacggtata ctggctgtcg gtccagttga cgctcccctc gatgcgcccc
     3061 ggagacacga cggtgccggt caactcgtgc gaaaggcgtt gggtaaaata gatgtcaccc
     3121 ttgatgagcg acgatgtaaa accgatggta aaagccggtt tgtcgtcggc atcgtggtcg
     3181 aatatgcggg catcatccgc tgcggtcggc atcagatcgg tctcgacatt gtccatcttg
     3241 tgcatgccgc gcagttccca tgaacggttc tcaacaaagg ttatcgtgcc accgttgtcg
     3301 gtgacggtga tgtcgggttt cgggttcgac aactcaccgg gccgccaata ccagtagata
     3361 tcgcagaacg gttggtaaaa gatggtgtgg ccttcaccgc taatcgggtc aaccgtagtg
     3421 ttgtctatct tgcagatctt ctgctctatc ttgagttcgc cgttctcgat cgatatatgc
     3481 cccaatttat agcgggtggt ggtcgaagga acatcgccga tacccgtgtg ggtgttgctc
     3541 ttgagaacga gtttctcggc ccaggtcccc acgaacgggt caagcagggg gtgcccctcg
     3601 tcgggctgtt cggtgtcctc gtcgacaagg tcgtcgctca tatcgtccgt cgcctcgtcg
     3661 ccgcaacaga catcgaactc taccggggcg gtgtcctccg cctcgtcctc atccggcgtc
     3721 tcgtcgatca ccgggatgtc gtccaccgct atctcgtcgt tcaccgtctc atcgggcacc
     3781 gcgttgtcgg tcgccgcatt gtcggggacc gtgttggtcg cattgtcgcc gcaggcggcc
     3841 agcagtatca cgcacgctac ggcgaagaac aaccgggaca ctttcatggg acgctccttc
     3901 ataacggtca ataaagtatc ttcacgaaca gactggtgcc tacgaggaac ccgccgctcc
     3961 ggaacccggg aaagccgggg ttgttggtct gcagaccctg cgccgaggcg aacgggacat
     4021 ccggatccat gtagttgttg gcgtcatcgg ggaattcatc gacgataccc cccgcatcct
     4081 cgccgcgcga aaggtccttc atcgtggtgt ggtacaaaag ccactgcatc tgggcctgca
     4141 caccgcccgc gacggtgacc ttctcgaact cgaagtattc ggtccacccg gcggcccaga
     4201 tgagccggtg gttatccaca taggtctcgc gccccgtctg ttcggggacc ggcgactgga
     4261 cgaactggaa atcgaacccg acctgtcgct cctcgtgttt gaggtgcaga cccgcgttca
     4321 cgctcgccgt ctcgcgccac ttgtcggtcg gtttggttcc gtggcggttc tcgtaggtgg
     4381 accaatggtg ccacatgccg ccgaggacgg gggcgagttt ataatcgtgt ccgatgtcga
     4441 aagcatacga tatcccgccg ccgacgcgga gcggttcgta cccgtaggtc atcgaaccgg
     4501 cgctcccgag atagcgttga tcgtcgtctt cgtagtccca gccccacacc tgcgtcttgt
     4561 tctgcagttc tatctggccg ttgtccgact gcag
//
LOCUS       DAOQUE010000208         4584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_20966_length_4584_cov_3.451976, whole genome shotgun sequence.
ACCESSION   DAOQUE010000208 DAOQUE010000000
VERSION     DAOQUE010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20966_length_4584_cov_3.451976"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..1286
                     /locus_tag="PKH10_07340"
     CDS             327..1286
                     /locus_tag="PKH10_07340"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNT27975.1"
                     /translation="MRETYGRFESASGGAIAYTVWSPDDGSAVRRVMQVAHGMAEHAG
                     RYRHFAEFLTRHGFAVYAHDHRGHGRSVLPGEPRGFFAERDGWDAVVDDAREMTRIAK
                     ERHPGLPLFLLGHSMGSFIARSYAIRFGGELSGLLLSGTAGLPPWGEKWAVLAIVRFC
                     SAVWGIRTPCSFLHNHSLDTYNRPFVLPGSNSRYHWLSRDPAVAHGFDADPLCGGVFP
                     AAFFRDLADGMLFMSDPAEVTKMPKELPTLFFSGDRDPIGRNGAGVREAYHLFLGAGM
                     KDVTMTLYPGSRHEPLNEIDREKVFDDILAWLDVQRASHPSNS"
     gene            complement(1256..2341)
                     /locus_tag="PKH10_07345"
     CDS             complement(1256..2341)
                     /locus_tag="PKH10_07345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNT27976.1"
                     /translation="MTVPPDIPRQRRQTRWRTLFFITFGALLIVAAMNLFCPLRGPDT
                     APTPVTPGAPATPAAPIDPLVTTPSPATPQTAPEKARPDAYALSFLLAASFYNTFADN
                     EEVARLAQETGIRKLAERLSAHVARNLVWNLDMRKEVYPGDELDLVFRVVPPEEQAKR
                     ADTPDEIELLAMRYRSVKLAKEFRIYRFKASDRQFPAYYNADGTPVEKALKRSPLKDW
                     IQITSLLKDRRPRHDGIDFKAPVGTPVYAPWGGIVEKTNWKTRYNGYSLLARLDTSPA
                     VQMILLHLDKVHLKPGQRFAAGDHIADVGNTGRSFGPHLHYQLQHEARKIIDPIKYHG
                     TVTAKLPAGDAGAFLKTVQEFDGWLAR"
     gene            complement(2438..3154)
                     /locus_tag="PKH10_07350"
     CDS             complement(2438..3154)
                     /locus_tag="PKH10_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27977.1"
                     /translation="MRVNSAIIAALLAATVFLSCEQVISLDRSLSQAEGSADGEKDST
                     DEGAAGGNPAEDIAEGADSPSGDMYSDSVAASDADNAVPMSDDYMNGDGSEDELATDS
                     ATYPDSASSDGTGMSDADTAESNDDDPTAVFSVGDCGCGDSPAYEPICCDGVTTVFNT
                     CFANCINVHTGQCATRTDGPCAAAIDNDDSEVSDDDAVTGTCGCVPTDMNAWCCDGGA
                     RYISQCTATCECGGDITPCR"
     gene            complement(3144..4322)
                     /locus_tag="PKH10_07355"
     CDS             complement(3144..4322)
                     /locus_tag="PKH10_07355"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNT27978.1"
                     /translation="MNTKHLSNAELIAICRQEGEPSPETIEHLAACDECAARLEARLR
                     NEPMLPASSDHDAAIDRIARISFDHLDEARPGLFAMLFTLPRLAVGAAAAALILIAAY
                     VGIGAPATVEEEKIADADAPAPRIAPTLGATPRLFPVGTTIAHGRATILVKGPASLAA
                     DGAEELRLAKGTVEVSIEKGNDFRIHTADRYLVRVLGTRFTLDTDGTKLAVTVAEGLV
                     EVIDGRTNDSIALSGGMAHTFTGETKEIATVAAKQPRAVRPAPGVDTAADEQPAATSF
                     LRNGRDALKTQDATAAMDWFEKELAGGPEKDKALFEIVRLHERDGRFDDMLRVLKAHN
                     DIVDGDTVFHEELLIKACKAEVKTGETTFPSCKKYLRVFPEGYKNGEIRELLENGDAR
                     "
     gene            complement(4319..>4584)
                     /locus_tag="PKH10_07360"
     CDS             complement(4319..>4584)
                     /locus_tag="PKH10_07360"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNT27979.1"
                     /translation="NLPSENEDDLSPAATLLGAERREQVRVTLGRISTEKRMALVLFE
                     MEGLTLREIAAMLKVPLQTVWSRVLNGRKDMAKMLTRAYGVAS"
BASE COUNT          695 a         1379 c         1556 g          954 t
ORIGIN      
        1 cggagcggat gatcatcgcg tcggcggcgg ggaccgccgc gagcaggtcc ttcgggtcct
       61 tgtagttctc gagtttgatg agttcgtagt tcgcctcttt gcagacgacc gcgatcttgt
      121 cgaccgcctc tttgacgaat gctttctcgg tagcgagcag gatcttcttc atggcttctc
      181 ctcctaaggt gggtggttga tcggtcacgc ggtccgaatc tagcatgccg cgttcggcat
      241 tgtcaacttt aaccgttccg cctcatcttg tctttttttg tcgggtgtgc tacatgagga
      301 gcgccgatag gcgaagggag tgcgacatga gagagacata tggtcgattc gagagcgcca
      361 gcggcggcgc tatcgcttac acggtatggt ctcccgatga cgggtcggca gtgcgtcgcg
      421 tcatgcaggt ggcgcacggc atggcggagc atgccgggcg ataccgtcac ttcgccgagt
      481 tcctcacccg gcacgggttc gcggtctacg cgcacgatca ccgggggcat ggtcgttcgg
      541 tgcttccggg tgagccgcgc ggctttttcg ccgaacgcga cgggtgggac gccgtggtgg
      601 acgacgcccg cgagatgacc cgcatcgcga aggaacgcca tccgggtctc cctcttttcc
      661 tgctggggca cagcatgggg tcgttcatcg cccgctccta tgcgatacgg ttcggcggcg
      721 agttgtcggg gctcctcctt tccggcaccg ccggtctgcc gccatgggga gagaaatggg
      781 cggtgctcgc catcgtccgc ttctgttcag cggtgtgggg catccgcacg ccctgttctt
      841 tcctgcacaa ccattcactt gacacctata accgcccgtt cgtcctcccc gggtcgaaca
      901 gccggtatca ctggctgtcg cgcgatcccg cggtggcgca cggcttcgat gccgacccgc
      961 tctgcggcgg cgtattcccg gccgcattct ttcgcgatct tgccgacggg atgttgttca
     1021 tgagcgaccc ggctgaggtg acgaagatgc cgaaagagct cccgacactt ttcttctcag
     1081 gcgaccgcga ccctatcggt cgcaatggtg cgggggtccg cgaggcgtac catctctttc
     1141 tcggagcggg gatgaaagat gtgacgatga ccctttatcc cggatcgcgg cacgagccgc
     1201 tgaacgagat cgaccgggaa aaggtgttcg atgatatcct tgcgtggctc gatgttcagc
     1261 gagccagcca cccgtcgaat tcctgaacgg tcttcaggaa cgccccggcg tcccccgccg
     1321 gtagtttcgc ggtcacggtg ccgtgatatt tgatcgggtc gatgatcttg cgggcctcat
     1381 gctggagttg gtagtggaga tgcgggccga acgatctacc ggtgttgccg acatcggcga
     1441 tgtggtcgcc cgccgcgaaa cgctgtccgg gcttcagatg gaccttgtcg aggtgcagga
     1501 gtatcatctg gaccgccgga gaggtgtcga gccgggcgag aagcgaatag ccgttgtagc
     1561 gggtcttcca gttggtcttt tcgacgatgc cgccccacgg ggcatagacg ggcgtgccga
     1621 ccggcgcctt aaagtctatc ccgtcgtggc gcgggcggcg gtccttgagg agcgaggtta
     1681 tctgtatcca gtccttgaga ggtgatcgtt tcagggcctt ttcgaccggg gtgccgtccg
     1741 cgttgtagta ggcggggaac tgtcggtcgg aggccttgaa acggtagatg cggaactcct
     1801 tggcgagttt cacggaccgg tagcgcatcg cgagcagttc tatttcgtcg ggggtgtcgg
     1861 cccgtttcgc ctgttcctcg ggcgggacga cgcggaacac gaggtcgagt tcgtcgcccg
     1921 ggtagacctc cttgcgcatg tcgaggttcc agacgaggtt gcgcgccaca tgggccgaga
     1981 gccgttcggc cagtttccgg atgccggtct cttgggcgag ccgggcgacc tcttcgttgt
     2041 cggcgaaggt gttgtagaac gaggcggcca gaagaaaaga aagagcgtag gcgtccggtc
     2101 ttgctttttc aggcgcggtc tgcggcgtgg cgggtgaggg ggtggtgacg agcgggtcga
     2161 taggagcggc cggcgtggcc ggtgccccgg gtgtgaccgg cgtcggcgcc gtatcggggc
     2221 cacgaagcgg gcagaagagg ttcatcgcgg cgacgatcag gagcgcgccg aaggtgatga
     2281 aaaaaagcgt tcgccagcgg gtctgccgtc tctggcgcgg gatgtcgggc ggcacggtca
     2341 tatcgctccg gtgcgtcgcg tgggaataac aacgggcgca tcatgcccgt tcgaaaaaag
     2401 gaaagccaga aatttttaag aaaatattac ccgggtctca acggcaaggg gttatgtcgc
     2461 cgccacattc gcaggtcgcg gtgcactggc tgatgtagcg agccccgccg tcgcagcacc
     2521 acgcgttcat gtcggtcggg acacaaccgc aggtccccgt cacggcgtcg tcgtcggaca
     2581 cttcgctgtc gtcgttgtcg atcgccgcgg cgcaggggcc gtcggtgcgg gtggcgcact
     2641 ggccggtgtg gacattgatg cagttggcga agcaggtgtt gaataccgtc gtgacaccgt
     2701 cgcagcagat cggttcgtac gcggggctgt cgccgcaccc gcagtcaccg acggagaaca
     2761 cggcggtcgg gtcgtcgtca ttcgattcag cggtgtcggc atcgctcata ccggtgccgt
     2821 ccgaggaggc gctgtcgggg taggtggcgc tgtcggtcgc cagttcgtcc tccgagccgt
     2881 cgccgttcat gtagtcgtcg ctcatcggga ccgcgttgtc ggcatcgctc gcggcgaccg
     2941 agtcgctgta catgtcgccg ctcggtgaat cggccccttc ggcgatatcc tcggccggat
     3001 tccctcccgc cgcgccctcg tcggtggagt ccttttcgcc gtcggcgctt ccctcggcct
     3061 gcgataaact ccggtcgagc gatatgacct gttcgcagga taggaagacc gtcgccgcca
     3121 gcagcgccgc tatgatcgcg ctgttaacgc gcatcgccgt tctccaacag ttcgcgtatc
     3181 tcgccgttct tatacccttc ggggaaaacg cgcaagtatt ttttgcacga agggaacgtc
     3241 gtctcaccgg tcttcacctc cgccttgcag gccttgatga gcagttcttc gtggaacacc
     3301 gtgtcgccgt cgacgatgtc gttgtgtgcc ttgagcaccc tcagcatgtc gtcgaaccgc
     3361 ccgtccctct catggagccg gacgatctcg aagagcgcct tgtccttctc ggggccgccg
     3421 gcaagttcct tttcgaacca gtccatggcc gccgtcgcgt cctgtgtctt gagggcgtcg
     3481 cgaccgttcc ggaggaagga tgtggcggcg ggctgttcgt ccgcggcggt gtcgaccccc
     3541 ggcgccggtc tgacggcgcg cggctgtttc gcggcgaccg tcgcgatctc tttcgtttcg
     3601 ccggtgaagg tatgtgccat gccgcccgaa agcgcgatcg aatcgttcgt gcggccgtct
     3661 atcacctcga cgagcccttc ggcgaccgtc acggcgagtt tcgtcccgtc ggtgtcgagc
     3721 gtgaagcggg tgccgagcac acggacgagg taccgatcag cggtgtggat gcggaaatcg
     3781 ttccctttct ctatggatac ctcgacggtg cctttcgcaa gccgcaactc ctcggcgccg
     3841 tcggcggcca gcgaggcggg gcccttcacg aggatcgtcg cccggccgtg ggctatcgtc
     3901 gtgccgacgg ggaagaggcg cggggtcgcg cccagcgtcg gagcgatccg gggcgccggg
     3961 gcatcggcgt cggcgatctt ctcttcttca acggtcgcgg gggcgccgat cccgacatag
     4021 gcggcgatga gtatcaacgc cgccgccgcc gctccgaccg cgagtcgtgg cagggtgaag
     4081 agcattgcga agaggcccgg gcgcgcctcg tcgagatggt cgaaactgat ccgcgcgatg
     4141 cggtctatcg ccgcgtcatg gtcggacgat gcagggagca tcggctcgtt gcgcaaccgc
     4201 gcctccagac gggcggcgca ttcatcgcag gccgcgaggt gctcgatggt ctccggcgag
     4261 ggttcgccct cctgccgaca gatcgcgatg agttcggcgt tggagaggtg tttcgtgttc
     4321 atgacgctac cccgtatgcc ctggttaaca ttttcgccat gtccttgcga ccgttcagga
     4381 cgcgcgacca gaccgtctgg agggggacct tgagcatggc cgctatctcg cggagggtga
     4441 gcccctccat ctcgaacagc acgagcgcca ttcgtttttc cgtgcttatc ctgccgagcg
     4501 tcacgcggac ctgttcgcgg cgttcggcgc cgagcagggt cgccgccggt gacaggtcat
     4561 cctcgttctc ggaagggagg ttgg
//
LOCUS       DAOQUE010000209         4580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_20989_length_4580_cov_5.025856, whole genome shotgun sequence.
ACCESSION   DAOQUE010000209 DAOQUE010000000
VERSION     DAOQUE010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20989_length_4580_cov_5.025856"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..1438
                     /locus_tag="PKH10_07365"
     CDS             254..1438
                     /locus_tag="PKH10_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT27980.1"
                     /translation="MYQRPLFDKVIKRLKEPRKFIQVLAGPRQVGKTTLITQVCERLS
                     TPYAYASADGAENHLWIEQQWQAARLRLAGSPKEEFLLVLDEIQKVPGWTDAIKRLWD
                     EDSREKRAIKLVILGSSPLLIKSGLSESLGGRFELIRCTHWGFAEMRDAFGIDLDHYL
                     FFGGYPGTAGLIGDEKRWRSYVREALIETTISKDVLLLNRIDKPALLRTLFLLGAGYS
                     GRILSYTKMQGQLQDAGNTTTLAHYLSLLDGAGMLTGLEKYATQGVRRRGSSPKLQVY
                     NNALFSAVRGDTFTEVRHDPERWGRTVESAVGSFLQNEATARDLSLFYWRESDAEVDF
                     VLTKGDRLAAIEVKSGAVRERPAGLDMFVAMTKKKVRPYLVGSGGIPLDEFFSIDLDA
                     LF"
     gene            complement(1487..2026)
                     /locus_tag="PKH10_07370"
     CDS             complement(1487..2026)
                     /locus_tag="PKH10_07370"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT27981.1"
                     /translation="MRSILGMMIVVVLIACGGDDGSDRLPDNSQPDIDTEQSDADTAP
                     VWVTDKTYAGLEWSPLPENEMTWQEAEDHCENMGGRLPNISELRKVIINCPGSTYGGA
                     CEVSDPDCLDSSCYSTKCSCDGFATTYSAFGDAKAAVWLWSSSPRVGYDHYAWGVNFY
                     NGNVSFSNKYSNNRVRCVR"
     gene            complement(2049..3128)
                     /locus_tag="PKH10_07375"
     CDS             complement(2049..3128)
                     /locus_tag="PKH10_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27982.1"
                     /translation="MELAEYPDYDYVPCAEREEEVRHNKAQLGDPWNYDLLSGITYLT
                     GTLDVQSDSTGDKDFTKLTGLRCIDGDMRIWYTDELESLHGLESLIRIGGNLDIEANK
                     ALSDISALSGITGTKGKVDLYGCPLMESLEGLHNIEKAQTISIETMNALKDLNGIRGL
                     QSVGTLVIYTATQLNTLAGLENVNKIGCVLQIVNTNISSLQGLSGLESMGTDCEDAVI
                     ILGNNHNLSSLEGMDNLETITGTFQLISNSILADTRGLEKITGINGDIIIDNNDSLKA
                     LTGLENLKTITESIRITGNLNLMDITALYNLESFGSDLGRDLVIINNPKLPTCQCEHL
                     RSLLMAKGWNGFYDFTGTDGTASCE"
     gene            complement(3804..>4580)
                     /locus_tag="PKH10_07380"
     CDS             complement(3804..>4580)
                     /locus_tag="PKH10_07380"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT27983.1"
                     /translation="LLGDMYFKGEGGPVNTAKARNAFEKACGGGAAHGCNMRAFMWLK
                     GIASKVDKKKAYDHFLDYCDDGNGEACLNLGLVLTDGDGQPVDLKRASEAFDRACFNG
                     SHEGCYRLAGMLVSGKGIAVNKKRAREYLQKACDWGNGDACGMLAFMWYNGEGGDLNK
                     DKARIYNEKACGKQRSIACFNLAVYYYQGEEGSANKERARQLFSRACELGNNDGCFLV
                     GAMHFTGEGGPVDIENAKKFVKKACTNKIEDACLFLKNNF"
BASE COUNT          979 a         1309 c         1230 g         1062 t
ORIGIN      
        1 cgccgtgacc gacaacgccg ctgccgacac cggcggcgcg accgacgagg atgcgctctg
       61 ggaagaggag gaggatatcg gttgcgggtg cgcgcttatc gggtgatctt tccctagacg
      121 atgcgcgtat cgtccgcgtc tctcctcgcc cgaaataaat cttgacttcc ttgcccgtgt
      181 cgggcatatt tactcaatgc gttgagcaga aatactcatt aggttgagta atttattctg
      241 acggaggcgg aacatgtatc aaagaccgtt gttcgataaa gttatcaagc ggttaaaaga
      301 accgaggaag ttcatccaag tcctcgcggg accgcgtcag gtcggcaaga caacgctcat
      361 aacgcaggta tgcgaacggc tttcgacccc gtatgcttat gcatcggccg acggcgcgga
      421 gaatcaccta tggatcgaac aacagtggca ggcggctcgc ctccggctgg ccggatcgcc
      481 gaaagaagag ttcctgctcg ttctggacga gatccagaag gtgccgggat ggaccgatgc
      541 cataaagcgt ctgtgggatg aggactcgcg tgagaaaagg gccatcaaac tggttatcct
      601 cggttcttcg cctttgctca tcaagagtgg tctgagcgag agcctcggag gtcgcttcga
      661 actcatccgg tgtactcatt ggggctttgc cgaaatgcgc gatgcgttcg ggatcgatct
      721 agatcactat cttttcttcg ggggatatcc gggaacggcc gggctcatcg gcgacgaaaa
      781 gcggtggcgc agttatgtcc gcgaagcgct gatagaaaca accatctcga aggatgtcct
      841 gctcctgaac cggatagaca aaccggccct cttgcgcacg ctgttccttc tcggagccgg
      901 gtattcgggc cgcatccttt cctacaccaa gatgcagggg cagttgcagg atgccggcaa
      961 caccacgacg ctggcgcact atctctccct cctcgacggg gcgggaatgc tgaccggcct
     1021 ggaaaagtat gcgacacagg gggtgcgccg tcggggatca agtcccaagt tgcaggtgta
     1081 taacaacgct cttttttccg cggtgcgggg cgataccttc accgaggtgc gccacgaccc
     1141 ggaacggtgg ggacgcaccg ttgaatcggc ggtgggatct tttttgcaga acgaggcgac
     1201 cgcgcgcgac ctgagtcttt tttactggcg cgaaagcgac gccgaggtcg attttgtatt
     1261 gaccaagggg gaccgtcttg cagcgatcga ggtgaaaagc ggcgccgtga gggaacgccc
     1321 ggcgggattg gatatgttcg ttgccatgac gaagaaaaag gtcagaccct acctagtcgg
     1381 aagcggaggc ataccgcttg acgagttctt ctcgatcgat ctggatgcgt tgttctgagc
     1441 ggaaccccgg cctcctagca tgccccgagg cgacggtcgg cgccggtcat cttacacaac
     1501 gcacccgatt gttgctgtac ttattgctga aactcacatt gccgttatag aaattaacac
     1561 cccacgcgta gtggtcgtag cccacacggg gcgaagagga ccagagccag accgccgcct
     1621 tggcgtcacc aaacgccgag tatgttgtgg caaaaccgtc acaagagcat tttgtgctgt
     1681 aacaactact atccagacaa tcgggatcgg agacctcgca tgcgcctcca taggtgctac
     1741 cgggacagtt gatgatgacc tttcgcagtt cactgatgtt cggcagtcgg cctcccatgt
     1801 tttcgcagtg gtcctcggcc tcttgccatg tcatctcgtt ttccggcaag ggcgaccatt
     1861 cgagtccggc gtaggtttta tcggtgaccc atacgggggc tgtgtccgca tcggactgct
     1921 cggtgtcaat atcgggctgg gagttgtcgg ggagtcgatc gctcccgtca tccccgccac
     1981 aggcgatgag taccaccact atcatcattc ccaagatcga tctcacgacg gcccgctctc
     2041 ttgtgctctc attcacaact cgcggtccca tcggtccccg tgaagtcgta gaagccattc
     2101 cacccctttg ccatcaacag ggagcggaga tgttcgcatt ggcaggtcgg caacttggga
     2161 ttgttgatga tgaccagatc tctccccaga tcgctcccga atgattccaa gttataaagt
     2221 gcggtgatgt ccatgagatt caggttcccg gtgatccgga tcgattcggt tatcgttttc
     2281 agattctcaa gtcccgtcag tgcttttaga gaatcgttat tatcaatgat gatgtcaccg
     2341 ttgatccctg tgattttttc aagaccacgc gtgtccgcaa gaatagaatt tgaaataagc
     2401 tgaaaggttc cggttatggt ttcgagattg tccatcccct ccaaagaaga taggttatga
     2461 ttgttcccta gaatgatcac ggcgtcttcg caatctgttc ccatgctttc aaggcccgat
     2521 aagccctgaa gggaagaaat atttgtatta acgatttgta aaacgcatcc tattttgtta
     2581 acattttcta gcccggcaag cgtgttaagt tgagttgccg tgtatatcac aagagttcct
     2641 acagattgca atcctcgaat gccgttcagg tctttcaatg cattcattgt ttctattgaa
     2701 atagtttgcg ctttttcgat attgtgtaag ccttccagag attccatgag aggacatcca
     2761 taaaggtcca ctttcccttt tgtccctgtt atgcccgata atgccgatat gtcagaaagg
     2821 gctttgttcg cttctatatc caaattcccc cctatccgta tcaaactctc cagcccatgc
     2881 aaactttcca gttcatcggt gtaccagatg cgcatatcgc cgtctatgca ccggagcccc
     2941 gtcagtttgg tgaaatcctt gtcccctgtc gaatcgcttt gcacatccag cgttcccgtc
     3001 agataggtga tccccgataa gagatcataa ttccacgggt cacccagttg cgctttgtta
     3061 tggcgaacct cttcttcccg ttcggcacaa ggaacataat cgtagtcggg atattccgca
     3121 agttccacga catcgtcccg cgtatcttcc ggacaggagg cctgccacga ttgcgcgccg
     3181 gcaacctccg ttacacaacg gatactcccc gtgggcgccg tcgtaaagta acgaccgatg
     3241 ctcgcatagg cgaaatctat accccaatag gtaagcgatt caccgggagt cgatgtccgg
     3301 gtccagaacc atgtgcgatc cccgaaaacc gagtaacctc cgtcctctct tttctcgcac
     3361 cccttgcacg atttgtccca attctgccac gattccgaag aaacccctat cgcgcaagaa
     3421 ccctcccata cagtgtgttc acaacccttt accaaggtgc gaagctccca aataaacggg
     3481 agacgaccct ctctagggca gaaatgtgcc gcctcctcat aggtgctaca gcagaacggt
     3541 tcgtcccacc aaaggagatc cccccggcgt cgccatcctt cacattgagg atcgggaccc
     3601 acgacgacat cagcgtccgg tacctcgtca aactcgggga caatatcgct ctcggacagg
     3661 tggtcggtgt cgcttgttcc gtaagaagaa ttgtgacagg aaaaagaaaa aagaagagat
     3721 agagcgaaaa gacacgagaa cgaaaaccgc acgaacggcc agccgcgtga aggtccggtt
     3781 ctcaagatcg gaagcccccc ctcctagaaa ttgttcttca ggaacaggca ggcgtcctct
     3841 atcttgttgg tgcacgcctt tttgacgaac ttcttggcgt tctcgatgtc gacggggccg
     3901 ccttcgccgg taaaatgcat ggcgccgacg aggaaacagc catcgttgtt gcccagttcg
     3961 caggcacggg agaacagttg gcgcgcccgt tccttgttgg cgctcccctc ttcaccctga
     4021 tagtagtaca ccgcgagatt gaagcaggcg atggatcgct gtttgccgca cgccttttcg
     4081 ttgtagatgc gcgccttgtc cttgttgagg tcgcccccct cgccgttgta ccacatgaac
     4141 gcgagcatgc cgcaggcgtc gccgttgccc cagtcgcacg ctttctgcag atattcgcgc
     4201 gccctcttct tgttcaccgc gatccccttg ccgctcacga gcatgccggc aagccggtag
     4261 cacccctcgt gggagccgtt gaaacaggct cggtcgaacg cctcggaggc ccgcttgagg
     4321 tcgaccggct ggccgtcccc gtcggtcagc acgagaccga ggttgaggca cgcctcgccg
     4381 ttcccgtcgt cgcagtaatc gaggaaatgg tcgtacgcct tctttttgtc cactttgctc
     4441 gcgatgccct tgagccacat gaaggcgcgc atgttgcacc cgtgggccgc gccgccgccg
     4501 cacgcctttt cgaaggcgtt ccgcgccttg gcggtgttga ccgggccgcc ttcccctttg
     4561 aaatacatgt cgccgagcag
//
LOCUS       DAOQUE010000210         4568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_21070_length_4568_cov_4.980722, whole genome shotgun sequence.
ACCESSION   DAOQUE010000210 DAOQUE010000000
VERSION     DAOQUE010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21070_length_4568_cov_4.980722"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKH10_07385"
     CDS             <1..204
                     /locus_tag="PKH10_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27984.1"
                     /translation="LFRSPCDRHGAQDHHHHEGSHRFLHKKDTNGPAWSPMCYPSFGM
                     PQYIPTVGVMQKIRLKFFLFISE"
     gene            complement(220..1140)
                     /locus_tag="PKH10_07390"
     CDS             complement(220..1140)
                     /locus_tag="PKH10_07390"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNT27985.1"
                     /translation="MSDPRIIITLGDVNGIGPEILLKFHKMRPDLPILCVGDRTALLY
                     WSRELGLPFHFEVIDLNVRYHPEPRTVSAVAGAAAAQAVERGYEEAKNRGLPLVTLPL
                     SKEAVALSRSGFTGHTELLATLDGKTPDEVVMFLTGPRLSVVPLTRHIPLSAVSQALT
                     VERVVRQVTVARAWFGERHGRAPKIWLAGLNPHAGEGGRIGVEEVSTLVPALIELRRN
                     GIDIEGPLPADTMFATGPAAGVDLFFGCYHDQVLAPFKLLHFDEGINATLGLSVLRAS
                     PDHGTAFPIAGKSVASEKSFAAAVDWALPH"
     gene            complement(1137..2462)
                     /locus_tag="PKH10_07395"
     CDS             complement(1137..2462)
                     /locus_tag="PKH10_07395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNT27986.1"
                     /translation="MPLFAVILLLFLFSCDDAAVSKKPDPLVFISIERNEGIQQTTLY
                     LDSGAVNRVFATVDASFALHADDLAAVSEAVAALDLEKECTEPTGDTDTPLLTYRRGT
                     VTTTCVVRSLKAALDSLLLIPERTMLWPFRPQNEPHPIGHTILSFQDYSPLSGDEYFH
                     PGVDILMPENSVVYNILPGRVVKYDYYDLSGGEAQDRMYFEVVVETQNGLTIQYHHID
                     PDSVSDTVKDAKQSGEVLPAGAEIGHIVSWPVVETGYSNEPFHHLHLNIFTRDLLPLN
                     GLQMLIAHPDTIPPVIESVTLVNGAVSAVLDPAALTADFHVIFEGYDMLDDNVWPLPP
                     RRYTVTVKDGKGKSLLERNSYDYLAALSTKKEDFACDYYLCGAAGMSTKGDYGPRAMF
                     VNVTAFDEAGAAGAPIAANELGNGERTLTLTACDEYDNCATKKLGMVIP"
     gene            complement(2501..2716)
                     /gene="rpsU"
                     /locus_tag="PKH10_07400"
     CDS             complement(2501..2716)
                     /gene="rpsU"
                     /locus_tag="PKH10_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNT27987.1"
                     /translation="MKKAKSIGPLEVSVTDDLERAIKILKKKMALEGLFKELKKRRHY
                     EPPSIRDRRKREEAERRRRKNLRKKRV"
     gene            complement(2814..3329)
                     /locus_tag="PKH10_07405"
     CDS             complement(2814..3329)
                     /locus_tag="PKH10_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNT27988.1"
                     /translation="MKSNRSPGTLRRSVEERMDTVRDIIEFLAARRETLSVAESCTGG
                     LVAAAIVAIPGASRVFTEGIVAYADEVKTARLRVEPATIERHGAVSIETVRAMLAGLS
                     ADARIAVSGIAGPDGGTAVKPVGTVVIGARYRDEERIVLHCFSGDRAVVRQAAVREAF
                     TLLRALLAPTS"
     gene            3423..>4568
                     /locus_tag="PKH10_07410"
     CDS             3423..>4568
                     /locus_tag="PKH10_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT27989.1"
                     /translation="MASTNGRRSMSPVGAMLLLLFFMFAMPSASAAEEKRYKLAVMEI
                     EDRSGKFSKEVISNATEYLRSKMVSSNKYVVISKDRQQKQLIKEEKKESHKECYDQSC
                     RIQLGQALSADTILAGSITKFGGMFTLTVEIIDLAKEATIQGAEAEFENMEKGLKTAI
                     EEIAEQLITGRKRSSVQKAAANASVAATPVRAGAPANDLEGRYDQARDLERTDKGRAI
                     EIYEDIMDTAEKGSKYYLRAEKRIAELKGGAKPAAAASNPAAPRSTRYTVAENKVTDP
                     KTGLVWQRDNASNLNFEAAENYCRSLNLDGEMGWTLPTIDELRTLMSGCPNSAPGGSC
                     KVTNSCRSSGCASDPVCSCGPQGGPGEKGFYWEPGVWRYTGSSFGTFW"
BASE COUNT          908 a         1339 c         1449 g          872 t
ORIGIN      
        1 ctcttccgct ccccatgcga ccgtcacggc gcacaggatc atcaccatca cgaaggttct
       61 catcgtttcc tccataaaaa agacactaac ggccctgcat ggtcacccat gtgttatcct
      121 tccttcggta tgccacagta tataccgacc gttggtgtaa tgcaaaagat tcgcctgaaa
      181 tttttcctgt ttatatccga gtagttagga ggtcttcggt tagtgtggaa gtgcccagtc
      241 gaccgcggcg gcgaaactct tttcggaagc gacgctcttc ccggcgatgg ggaaggcggt
      301 cccgtggtcg ggcgaggccc ggaggacgga gaggccgagc gtcgcgttga tcccttcgtc
      361 gaagtggagg agtttgaagg gcgcaagcac ctgatcgtgg taacagccga agaagagatc
      421 gaccccggcg gccgggccgg tcgcgaacat cgtgtcggcc ggaagcggcc cctcgatgtc
      481 gatgccgttc cggcgcagtt cgatgagcgc cgggacgagc gtcgagacct cctcgacgcc
      541 gatgcggccc ccctcgccgg cgtgcgggtt cagccccgcg agccatatct tcggcgcgcg
      601 accgtgccgt tcgccgaacc atgcacgggc gaccgtcacc tgccggacga cccgttcgac
      661 cgtgagtgcc tgcgacaccg ccgagagagg tatgtggcgg gtgagcggca cgaccgaaag
      721 gcgggggccg gtcaggaaca tcacgacctc gtcgggggtt ttcccatcga gtgtcgcaag
      781 cagttcggtg tgtcccgtga agccggagcg cgagagggcg accgcctcct tggagagcgg
      841 gagcgtcacg agcggcaggc cacggttctt cgcctcctcg taaccacgct caaccgcctg
      901 cgccgccgcc gctccggcga cggccgagac cgtgcgcggt tcaggatggt agcggacatt
      961 gagatcgatg acctcaaagt gaaaaggaag tccaagttca cgcgaccagt acagaagcgc
     1021 cgtccggtca ccgacgcaga ggatcggtag gtcgggacgc attttatgga acttgagcag
     1081 tatctccggg ccgatgccgt tgacatcgcc gagcgtgatg atgatgcgcg ggtcactcat
     1141 gggatgacca ttcccagttt cttggtcgcg cagttatcgt actcgtcgca ggcggtcagc
     1201 gtgagggtcc gttcgccgtt tccgagttcg ttggccgcta tcggtgcacc ggcagccccg
     1261 gcctcgtcga acgccgtgac attcacgaac atcgcgcggg gaccgtaatc acccttcgtg
     1321 ctcataccgg cggcgccgca gaggtagtag tcgcaggcga agtcctcctt tttcgtcgag
     1381 agggcggcca gatagtcgta actgttccgc tcaagcaggg acttcccttt cccgtccttc
     1441 accgtcacgg tgtaacggcg cggcgggagc ggccagacat tgtcgtccag catatcgtag
     1501 ccttcgaaaa tgacatggaa gtcggccgta agcgccgcag ggtcgagcac cgccgaaacg
     1561 gcgccgttga ccagcgttac tgattcgatg accggcggga tggtgtcggg gtgtgctatc
     1621 agcatctgca gaccgttcag cggcaggagg tcgcgcgtga agatattcag gtgcaggtga
     1681 tggaacggtt cattgctgta accggtctcg accaccggcc acgagacgat gtgaccgatc
     1741 tcggctccgg ccgggagcac ctcgccgctt tgcttggcat ccttcaccgt gtcgctcacg
     1801 ctgtcggggt cgatgtggtg gtactggatg gtcaggccgt tctgcgtctc gacgaccacc
     1861 tcgaaataca tccggtcctg cgcctcgccg cccgaaagat cgtagtagtc gtacttcacc
     1921 acacgccccg gcaggatgtt gtacacgacc gagttctccg gcatgaggat gtcgacgccg
     1981 gggtggaaat attcgtcgcc cgaaagtggc gaatagtcct gaaaactcag aatggtatga
     2041 ccgatcggat gtggctcgtt ctgcggtcta aagggccaca acatggttct ctccggtatc
     2101 agaaggagcg agtcgagagc ggccttcagc gatctcacca cgcaggtcgt cgtgaccgtg
     2161 ccgcgccggt aggtgaggag gggcgtgtcg gtgtcgccgg tcggctccgt gcactctttc
     2221 tcgaggtcga gcgccgcgac cgcttcgctt accgccgcga ggtcgtcggc gtgtagggcg
     2281 aacgatgcgt cgactgtcgc gaagacgcgg ttcaccgctc cgctgtcgag atacagggtc
     2341 gtctgctgga tgccctcgtt ccgttctatc gatatgaaga cgagcgggtc aggttttttc
     2401 gatacggcgg catcatcgca ggaaaaaaga aaaagaagga gtatcaccgc gaacagaggc
     2461 accacgacct cccgtgagaa gcccggtatg cggggaaagg ctacacgcgt tttttgcgca
     2521 gattcttgcg gcgacgccgt tcggcctctt cgcgtttacg gcggtcacgg atgctcggcg
     2581 gctcgtaatg acgccgtttc ttgagttcct tgaacagacc ttcgagcgcc atcttcttct
     2641 tgaggatctt gatcgcgcgt tccagatcgt cggtcaccga aacttcgaga gggccgatgc
     2701 tcttcgcttt cttcatgctt ggttcaccac ctttcatagc gggcttacgg gttaataaaa
     2761 cagcacaaaa cggccccact gtatccagaa aacggccgag cgcaagaatt tttttatgag
     2821 gtcggcgcca gcagggcgcg gagcagggtg aacgcctcgc ggacggcggc ctgccggacc
     2881 acggcgcggt cgccggaaaa gcagtgaagg acgatccgtt cctcgtcacg gtatcgcgcg
     2941 ccgatgacga cggtaccgac cggttttacg gcggtgccgc cgtcgggccc cgcgatgccc
     3001 gacaccgcga tgcgggcgtc ggccgaaagt cccgcgagca tcgcccgtac cgtctcgatg
     3061 ctcaccgcgc cgtggcgttc gatggtggcg ggctcgaccc ggagtcgcgc cgttttgacc
     3121 tcatcggcgt aggcgacgat accttcggtg aacactcgcg acgcccccgg tatggcgacg
     3181 atcgccgccg cgacaagacc gccggtgcaa ctctcggcga ccgagagcgt ttcgcgccgt
     3241 gccgcaagaa attcgatgat gtcgcgtacc gtgtccatcc gttcctcgac cgatcggcgg
     3301 agtgtaccgg gggaacgatt gcttttcaac ttttcacggt cgtggtattt ttttgacttc
     3361 gccagtcacg gggataaaat cgcttccgct gcgtccggtt gataagatgt ggagggtgca
     3421 aaatggccag cacgaatggt cgtcgatcga tgtccccggt cggcgcgatg ctcctgttgc
     3481 tgtttttcat gttcgcgatg ccttcggcgt cggcagccga ggagaagaga tacaaactgg
     3541 ccgtcatgga gatcgaagat cgatcgggca agttctccaa ggaggtcatc agcaacgcga
     3601 cagaatacct gagaagcaag atggtatcga gcaataaata tgtggtcatc tcgaaggacc
     3661 gtcaacagaa gcagctcata aaggaggaaa agaaggaaag ccacaaggag tgctatgacc
     3721 agtcgtgccg catacaactc ggtcaggcgc tctccgccga taccatactc gctgggagca
     3781 tcaccaaatt cggcggcatg ttcacgttga ccgtagagat catcgatctc gccaaggagg
     3841 cgacgataca gggagccgag gcggaattcg agaacatgga aaagggcctc aagacggcta
     3901 tagaggagat cgccgaacaa ttgataacgg gcagaaagcg ctcctcggtc cagaaggccg
     3961 ccgcgaacgc gtccgtcgcc gccacacccg tccgtgccgg ggctccggcg aacgacctcg
     4021 aaggacggta cgatcaggcg cgggacctcg agcggaccga taaggggcgg gcgatcgaga
     4081 tatacgaaga tatcatggac acggcggaga agggaagcaa atactaccta cgcgccgaga
     4141 aacggatcgc cgaactgaag ggcggcgcga agcctgccgc ggcggcttcg aatccggcgg
     4201 cacctcgcag tacccgctac acggtcgccg agaacaaggt gacggatccg aagacgggac
     4261 tcgtctggca acgcgacaac gccagcaatc tgaacttcga ggccgcagaa aattactgtc
     4321 ggtcgctcaa tctcgacggc gagatgggct ggacgctccc gacgatagac gaactgcgta
     4381 cgctgatgtc gggatgcccc aactcggccc ccggcggctc ctgcaaggtc acgaattcct
     4441 gccggtcgag cgggtgtgcc tccgatccgg tctgctcgtg tggccctcag ggtggacctg
     4501 gggaaaaagg gttctattgg gagcctggcg tctggcgtta taccggcagt tctttcggta
     4561 ccttctgg
//
LOCUS       DAOQUE010000211         4538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_21256_length_4538_cov_3.853892, whole genome shotgun sequence.
ACCESSION   DAOQUE010000211 DAOQUE010000000
VERSION     DAOQUE010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21256_length_4538_cov_3.853892"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2210
                     /locus_tag="PKH10_07415"
     CDS             <1..2210
                     /locus_tag="PKH10_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27990.1"
                     /translation="NHFRARKKIEEAGSPEGVAPTATTTAPASKTRYVRLPEFSTEDN
                     EHYRRAGRRNLTILALGALAIILTLWALWGGGTSGVDKMLKDPEATLWPLSVADFERR
                     SDEMRAIERRFPNEDAPRKALFYLTAFALLDYHLHEHKDILSQVRFYYTLSTQKDPSM
                     EQILRYIEGDPAISSLTREHALDSAVPGNAADLAAFTPRLQGPVTRSNLRTAWYDALL
                     LYRAARYDESLGIAEAMLTEFPALELPQKLRVAAIARKAVKQDAIADSTRALPDEQVR
                     QLTGTLARWRGESEERHLLSEAYVALGAAAHDPLLEREGFYLGCPGRPFCADILNRFM
                     NGGRTEDAKRMALYVREKKGTARNADDLKLVMLSSYRNGDHSNCFFAFKEIQQFFPAV
                     AQDAEVLRTAAICCEKENYLEEALALYEKIAATPRTPEQAAKIAEIRYLLSRDAQSAQ
                     ELKTLAEKYPDSATILNAHLSVLLRTDNRPEIQRALDRLYSLTGPDGRLTVIQRYLQA
                     GLTMRAVKLLMENRGRRDIDERLYDLYNAYFLFSDADAVAGKGAFDSTPFWKELREHY
                     AALSPETAETIGEKLEKLAPPPSQRCVPALLYLKAEAFRQSGDRPKTFSMIEGVLECD
                     RTYLPGLILAAEMAFYQGDRARALKAVKYLIEQAPFLSPGKLIYHNYLVLLLAELYIT
                     SGNEKKGLTVLTQDLSTAYPFGKREREKLQDIYDKSKLVLKKRIEQLVAERFR"
     gene            2227..>4538
                     /gene="leuS"
                     /locus_tag="PKH10_07420"
     CDS             2227..>4538
                     /gene="leuS"
                     /locus_tag="PKH10_07420"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNT27991.1"
                     /translation="MEYRPSELEPKWQKKWMDDRVFSYLGKSSDKPKYYMLEMFPYPS
                     GRIHMGHVRNYSIGDVVARYYVRRGYNVLHPIGWDAFGLPAENAAIEHKTAPAVWTYK
                     NIDNMKSQLKKLGFSYDWEREIATCRPEYYRWEQLFFIEMWEKGLVYKKESEVNWCDK
                     CATVLANEQVEEGQCWRCDSTVTKKKIDQWFFKITAYADELLTELDNLPGWPEHVKEM
                     QRNWIGKSEGAYIDFTTETGGKLTVFTTRPDTLMGVTFVSMAVEHPLADEILATAKDK
                     KEMAAFIEEVKSFHAANVRDDNYEKKGYFTGRYLTHPVTGAKVPLYFANFVLAGYGTG
                     VVMAVPAHDQRDFEFAQKYGLPVKVVIEPKDKKLDLPLTEAYTEPGVMTDSGEFTGTP
                     SDEGKKRVSQWLVAHDKGSVGINFRLRDWGISRQRYWGCPIPMVKCAKCGMVPVKKSD
                     LPVRLPEDVRFSGVNSPIKSMPSFYETTCPTCGGKAMRETDTMDTFVESSWYHLRYCD
                     PHNDKAPFDKGEAAYSMPVDQYIGGVEHAVMHLLYTRFFTKVLSDLGYVPFREPVKNL
                     LTQGMVCMESYKEGDEYLYPEEVEWRDGKPFSTKDGAPVTVGRIEKMSKSKKNVIDPD
                     KMVAKYGADTARLFILFASPPEKDLEWSADGVEGSSRFIARVWNIVTDNLDLIRTFPD
                     DPTEGGPFADLFRKTHQTIQKVRDEIERFHFNTGIAAIMELTNAAYKVKPATDEEKRV
                     FAFALRTIVGLLNPFAPHLTEELHRLCGGA"
BASE COUNT          988 a         1476 c         1306 g          768 t
ORIGIN      
        1 ccaaccattt ccgagcgcgt aagaagatcg aggaggcggg ctcgccggaa ggtgtcgctc
       61 ccacggccac gaccacggca cccgcatcga agacacgcta tgtgcggctt cccgaattct
      121 ccacggagga taacgaacac taccggcgtg ccggacgacg caacctgacg atactcgcgc
      181 tcggcgcact ggccatcata ctgacgctgt gggcgctctg gggaggcggc acgagcggtg
      241 tcgacaagat gctgaaagac cctgaggcga cactctggcc tctctcggtc gccgatttcg
      301 agcgacggag cgacgagatg cgcgccatcg agcggcgatt cccgaacgag gacgcgccgc
      361 gtaaagcact cttctatctg accgctttcg ccttgctcga ttaccacctg cacgaacaca
      421 aggacatcct ttcacaggtc cgattctact acaccctctc gacccagaag gatccgtcga
      481 tggaacagat cctccgttat atcgaggggg atcccgccat ctcatcgctc acccgcgaac
      541 acgctctcga ctccgcggtg ccggggaatg cggcggacct cgccgccttc acgccgcgac
      601 tgcagggccc cgtcacgcgg tcgaatcttc gcaccgcatg gtacgacgcg ctgcttctct
      661 accgcgcggc gcggtacgac gagtcgctag ggatcgccga ggcgatgctc accgagttcc
      721 cggcgctcga actgcctcag aaactgcgcg tcgccgccat cgcgcggaaa gcggtgaagc
      781 aagacgcgat cgccgactcg acacgggcac ttcccgatga acaggtgcga caactcaccg
      841 gcacactggc ccggtggcgc ggcgaaagcg aagagcgaca tctcctttcg gaagcctatg
      901 tggcgcttgg cgccgcggcg cacgacccgc ttctcgaacg ggaagggttc tatctcggct
      961 gtccgggcag acctttctgc gccgatatcc tcaaccggtt catgaacggg gggcgcaccg
     1021 aggacgccaa acggatggcg ctctatgttc gcgagaaaaa ggggacggcg cgcaatgccg
     1081 atgacctgaa actcgtcatg ctttcctcgt ataggaacgg cgaccacagc aactgttttt
     1141 tcgccttcaa ggagatccag cagttctttc ccgcagtcgc gcaggatgcc gaagtgcttc
     1201 ggaccgccgc catctgttgc gagaaagaga actatctcga ggaggcgctc gccctttacg
     1261 agaagatagc cgccaccccc cggacccccg aacaggcagc caagatagcc gagatacgct
     1321 acctcctgtc gcgcgacgct cagtcggcgc aggaattgaa aacgctcgcc gagaaatatc
     1381 ccgactcggc gaccatcctg aacgcccact tgtcggtact cctgcggacc gataaccgac
     1441 cggagatcca acgcgcgctc gaccggctct actcactgac cggccccgac ggacgactca
     1501 ccgttatcca gcgctacctg caggcggggc tcacgatgcg cgccgtcaaa ctcctcatgg
     1561 agaatcgtgg ccgacgcgat atcgacgagc ggctctacga cctctataac gcctacttcc
     1621 tcttctccga tgccgatgcg gtcgccggga aaggggcttt cgattccacc cccttctgga
     1681 aggaactgcg cgaacactac gcggcgcttt cccccgaaac ggccgagacc atcggcgaga
     1741 aactcgagaa actggcccct cccccctccc agcgatgcgt tcccgcgctt ctgtacctca
     1801 aggccgaggc gttccgtcag agcggcgacc gaccgaagac cttctcgatg atagaggggg
     1861 tactcgagtg cgatcgcacc tacctccccg ggctgatact cgccgccgag atggccttct
     1921 atcagggcga ccgggctcgc gccctcaagg cggtcaagta tctcatcgaa caggcgccct
     1981 tcctgtcgcc cggcaaactc atctaccaca attaccttgt gcttctgctc gccgaacttt
     2041 atatcacctc cggcaacgag aagaaggggc tcaccgttct cacgcaggac ctttccaccg
     2101 cctatccgtt cggtaaacgg gaacgggaga aactgcagga catatacgat aaatcgaaac
     2161 tcgtgctcaa aaagcgcatc gagcaactcg tcgccgagcg cttccgctag acaggaggca
     2221 atccccatgg agtatcgtcc ctcggaactg gaaccgaaat ggcagaaaaa atggatggac
     2281 gaccgcgtct tttcctatct cggcaaatcg agcgacaagc cgaaatacta catgctcgag
     2341 atgttcccct acccctcggg gcgcatccac atgggccatg tccgcaacta ttcgatcggc
     2401 gatgtggtgg cgcgctacta tgtccgtcgc ggctacaatg tgctccatcc catcggctgg
     2461 gacgcgttcg gtctgccggc cgagaacgcg gcgatcgagc ataagaccgc ccccgccgtc
     2521 tggacctaca agaacatcga caacatgaaa agccagctga agaaactggg cttttcctac
     2581 gactgggagc gcgagatcgc gacctgccgc cccgaatatt accgctggga acagctgttc
     2641 ttcatcgaga tgtgggagaa ggggctggtc tacaagaagg aatcagaggt caactggtgc
     2701 gacaagtgcg cgacggtgct ggccaacgaa caggtcgagg agggccagtg ctggcgctgc
     2761 gattcgaccg tgacaaaaaa gaagatagat cagtggttct tcaagataac ggcctacgcc
     2821 gacgaactgc tcaccgaact cgacaacctc cccggctggc cggaacatgt caaggagatg
     2881 caacgcaact ggatcgggaa gagcgagggg gcctacatcg acttcacgac cgaaacgggc
     2941 gggaaactga ccgtcttcac cacgcggccc gacacgctga tgggggtcac cttcgtgtcg
     3001 atggcggtcg aacatccgct ggccgacgag atactggcga cggccaagga caaaaaagag
     3061 atggccgcct tcatcgagga ggtcaaatcg ttccacgccg ccaatgtccg cgacgacaac
     3121 tacgagaaga aaggctactt caccggcaga tacctcaccc acccggtgac cggcgccaag
     3181 gtgccgctct acttcgccaa cttcgtgctg gcgggctacg gcaccggcgt ggtgatggcg
     3241 gtcccggcgc acgaccagcg cgatttcgag ttcgcgcaga aatacggcct gccggtcaag
     3301 gtggtcatcg agccgaagga taagaaactc gatctgccgc tcaccgaggc ctacactgaa
     3361 ccgggcgtga tgaccgactc gggcgagttc accgggacgc cctccgatga gggcaagaag
     3421 agggtgtcgc agtggctcgt cgcccatgac aaagggtcgg tcggcatcaa cttccgcctc
     3481 cgcgactggg gcatctcgcg ccagcgctac tggggctgtc ccatcccgat ggtgaaatgc
     3541 gcgaagtgcg gtatggtgcc ggtgaagaag agcgacctgc cggtgcggtt gccggaggat
     3601 gtccggttct ccggcgtcaa ttcgccgata aaatcgatgc cgtcgtttta cgagaccacc
     3661 tgccccacct gcggcggcaa agcaatgcgc gagaccgaca cgatggacac cttcgtcgaa
     3721 tcgtcgtggt accacctgcg ctactgcgat ccgcataacg acaaagcccc gttcgacaag
     3781 ggcgaggcgg cctattcgat gccggtggac cagtacatcg gcggggtcga acacgcggtg
     3841 atgcacctcc tctacacccg ctttttcacc aaggtgctct ccgacctcgg ctatgtgccc
     3901 ttccgcgaac cggtgaagaa cctcctcacg caggggatgg tctgcatgga gtcctacaag
     3961 gagggcgacg aatatctcta ccccgaagag gtcgaatggc gcgacgggaa gcccttcagc
     4021 acgaaggatg gcgctccggt gaccgtcggc cgcatcgaga agatgtccaa atcgaagaaa
     4081 aatgtcatag accccgacaa gatggtggcg aaatacggcg ccgacacggc gcgcctgttc
     4141 atcctgttcg cctcgccgcc cgaaaaggac ctcgaatggt cggccgacgg cgtcgagggt
     4201 tcttcccgct tcatagcccg cgtctggaac atcgtcaccg acaacttgga cctcatccgg
     4261 accttccccg atgacccgac cgaaggcggt ccgttcgccg acctattccg caagacccac
     4321 cagacgatac agaaggtgcg cgacgagata gagcgattcc acttcaacac cggcatcgcc
     4381 gcgatcatgg aactcaccaa cgccgcctac aaggtgaagc cggcgaccga cgaggagaaa
     4441 cgggtattcg ccttcgccct gcggaccatc gtcgggcttc tgaatccctt cgccccccac
     4501 ctcaccgagg aactccaccg cctctgcggc ggcgccga
//
LOCUS       DAOQUE010000212         4522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_21349_length_4522_cov_3.906873, whole genome shotgun sequence.
ACCESSION   DAOQUE010000212 DAOQUE010000000
VERSION     DAOQUE010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21349_length_4522_cov_3.906873"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PKH10_07425"
     CDS             <1..988
                     /locus_tag="PKH10_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNT27992.1"
                     /translation="CATACVGLVVTQRWHGRFTLDGLSGIQKFHRVPTPRIGGIVEGA
                     ELWFVPILNPDGYIYSHTTDRMWRENRRLRVDGVYGVDINRNYDAAWAANGYANGTEP
                     FSEPETRALRDLVTTPEGATALTDLPRGILTYHSYTQVLLYPWSSTSDPAPTRDLMRA
                     TAENMAALMLDSGGETYGVKQTSELYGVPIYGECTEWFYNATGQGTGFTVELRPATVE
                     GGGFQLDPIFIEPTARESIVAALYFIETIMNDSLDIDTDANGDGVIDYLEIPDAAPDE
                     TPDDDVLLDESDEPLMTDDAGPATDDAPLPADDDRRPAPHPATGGCSLTTLG"
     gene            complement(998..1423)
                     /gene="rpiB"
                     /locus_tag="PKH10_07430"
     CDS             complement(998..1423)
                     /gene="rpiB"
                     /locus_tag="PKH10_07430"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNT27993.1"
                     /translation="MFIAIASDHGGFALKKHLAALLEKEGHMVIDRGCPDESSVDYPD
                     LAALVAQDILVGNAEFGILVCGTGIGMSIMANRHPGVRAALVHRDDYAKLAREHNDAN
                     VICLGGRFVSKEDAERFVKIFLSTPFEGGRHQRRVEKLG"
     gene            complement(1545..2468)
                     /locus_tag="PKH10_07435"
     CDS             complement(1545..2468)
                     /locus_tag="PKH10_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT27994.1"
                     /translation="MTFHGIAPGERPLIIPVFITHRGCTNRCAFCDQEAIAGAGALPE
                     EVPGIVEKYRNWSAGDRPVELSFYGGSFTALPVRELGEYLQAGTELIARGLVGALRCS
                     TRSDTINAGMAKLLTSSGFTVVELGAQTMSDRLLTEMERGHTADDTRRAVRMLKEAGL
                     TVVLQFMSGYPDETPQDVATTCRAVAEMRPDAIRIYPFLPLAGTKIAERLQCKEARFD
                     PVAAIERAADIFLAAQESGIPTIRIGLPKENLVETPYPDNLAQVVVAEALVRLAKKGE
                     REFEIPAAWETSVAIAKKSGRWPQEAKVVFS"
     gene            2610..3908
                     /locus_tag="PKH10_07440"
     CDS             2610..3908
                     /locus_tag="PKH10_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27995.1"
                     /translation="MRHFVMSVSFFFVMLAMACASPEEDALCSFDEDCGDTALYFCDQ
                     QTGQCREKAGMTDTDDIGGGDEEKDESDMNDIEDGDETTESGLPDETVDETGSDALPD
                     NDTPSQWLDPVTGLTIDIEIDQLTLAWQNPTMAGLTDIKVVRNMIAFPQNIYDGDLLY
                     EGLDQTYADTSVDSGKSHFYSVFACYGELGCSEPAVMGGIPCYDRMDLVFVMDVSTSM
                     DYILSDLENEIGEVWNAVSQKIETTPRMGLTVFVDDVKVVNDGSTPYATVEQIKGDFH
                     SWYQHTSSNEQTQSSATNYDWPENSLDGLALTAKNFQWRDPAKALRIIILATDDTFRE
                     KPASFSSGIAAQHTYDETVALLQEGRIRVAAFAAKLGGSTGTADVQPGFFTTYNGKEP
                     IPAATGGEVFFIDDVKNGTLSLVDAITGFIETERCVAGER"
     gene            3925..>4522
                     /locus_tag="PKH10_07445"
     CDS             3925..>4522
                     /locus_tag="PKH10_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27996.1"
                     /translation="MNRFIAALLDEAIGRFEETRWKADLLLAALFRQRHIGKRDRALL
                     RELFFTYLRRRYLVESFMRAGLTRPDAVERAQGTTGVSGDLFHSFPPIYRDAAVARYG
                     EGADAALAWLNGRAPTTIRANVRRAGRDELRKRLATEGIRCLPTPVAPNGLIVTDGAS
                     QLTRSPLYEKGFFEMQDESSQIAVELVPGGAKTLLDLCA"
BASE COUNT          842 a         1523 c         1394 g          763 t
ORIGIN      
        1 ctgcgcgacc gcgtgtgtcg gtctcgtcgt cacgcagcgc tggcacggcc gcttcaccct
       61 cgacgggctc agcggcatcc agaagttcca tcgcgttccg acgccccgca tcggcggcat
      121 cgtggaaggg gccgaactgt ggttcgtccc gatactgaac cccgacggct acatctacag
      181 ccacaccacc gaccggatgt ggcgcgagaa ccgccggtta cgcgtcgacg gcgtgtacgg
      241 ggtcgacatc aaccgcaact atgacgccgc gtgggccgcc aacggctacg cgaacggcac
      301 ggagcccttt tccgagcccg agacccgcgc gctccgcgac ctcgtgacga cgccggaggg
      361 agcgacggcg ctcaccgatc tgccgcgcgg catactcacc tatcacagtt acacgcaggt
      421 ccttctctac ccgtggtcgt cgacctccga tccggcgccg acccgcgacc tcatgcgggc
      481 gaccgccgag aacatggcgg cgctcatgct cgacagcggc ggggaaacct acggtgtcaa
      541 gcagacctcc gaactctacg gcgtgccgat atacggcgaa tgtaccgagt ggttctacaa
      601 cgcgaccggg caggggaccg gcttcacggt ggagttgcgc cccgcgaccg ttgaaggggg
      661 cgggttccaa ctcgatccga tcttcatcga gccgacggcc cgcgagagca tcgtcgccgc
      721 gctctatttc attgaaacga tcatgaacga ctcgctcgac atagacaccg acgcgaacgg
      781 cgacggcgtg atagactatc tggagatacc cgacgcggcg cccgatgaga cgcccgatga
      841 cgatgtcctg ctcgacgagt ccgatgaacc gctcatgacc gacgatgcgg gaccggccac
      901 cgacgacgcg ccgctcccgg cggacgacga ccggcgcccc gcgccgcacc ccgcgaccgg
      961 cggctgttcg ctcacgacgc tggggtgatc cccgctctta cccgagtttc tcgacgcggc
     1021 gctggtggcg ccccccttcg aacggcgtgg aaaggaagat cttcacgaac cgttcggcat
     1081 cttccttcga gacgaagcga ccgcccagac agatgacatt ggcgtcgttg tgttcgcgcg
     1141 ccagtttcgc gtaatcgtcg cggtggacaa gcgccgcccg gacgccgggg tggcggttgg
     1201 ccatgatgga catgccgatg ccggtgccgc agacgaggat gccgaactcg gcgttcccga
     1261 cgaggatgtc ctgcgcgacg agcgccgcga ggtcggggta gtcgaccgaa ctctcgtcgg
     1321 gacagccgcg gtcgatgacc atgtgtccct ccttttcgag aagcgcggcg aggtgttttt
     1381 tcagcgcgaa accgccgtga tccgacgcga tggcgatgaa catggatgcc tccataaaac
     1441 cattcaaccg ttcactccct cgccccgttc ggggagaggg tcggggtgag ggtgttccat
     1501 agacctcacc gaatatccac gcagggcgtg ccgttggcgc gcccctacga aaacaccacc
     1561 ttcgcctctt gcggccaccg accgcttttt ttcgcgatgg cgacactcgt ctcccacgcc
     1621 gcgggaattt cgaactcgcg ctcccctttc ttcgccagtc tcacgagcgc ctcggcgacc
     1681 accacctgcg cgagattgtc gggatagggc gtctcaacga ggttctcctt cggcaggccg
     1741 atccgtatcg tcgggatgcc cgactcctgc gccgcgagaa agatgtcggc cgcgcgttct
     1801 atcgccgcga ccggatcgaa ccgcgcctct ttgcactgga gccgctcggc tatcttcgtg
     1861 ccggcaagcg gcaggaaggg atagatgcgt atcgcgtcgg gacgcatctc ggcaacggcc
     1921 cggcaggtcg tcgcgacatc ctgcggcgtc tcgtcggggt agccgctcat gaactggagc
     1981 acgaccgtca gccccgcctc tttgagcatc cgcaccgccc ggcgcgtgtc gtcggcggtg
     2041 tgcccgcgct ccatctcggt gaggagccta tcggacatcg tctgtgcgcc gagttccacc
     2101 accgtgaagc cgctcgacgt gagcagtttc gccatgccgg cgttgatggt gtcggaacgg
     2161 gtcgaacagc ggagagcccc gacgagtccc cgcgcgatga gttccgtgcc cgcctggaga
     2221 tattcgccca gttcgcgcac cggcagggcg gtgaaactgc cgccgtagaa cgacagttcg
     2281 accggacggt cgcccgccga ccagttgcgg tacttttcca cgatgccggg gacctcttcg
     2341 ggaagcgctc ccgcccccgc tatcgcctcc tgatcgcaga aggcgcaccg gttcgtacag
     2401 ccgcggtggg tgatgaagac cggaatgatg agcggtcgct cacccggcgc gatgccgtga
     2461 aaggtcattc agttccctcc ctataaggac cggaaaggtt gtatcagccg tcgcgccaag
     2521 agtcaaaaac tttcatcgcc gcgtacggat tattgctttt tacggacggc gcccctatac
     2581 ttgcgtcata tcgtcggtcg gagggcgcca tgagacattt tgtcatgtcg gtatctttct
     2641 ttttcgttat gctggcgatg gcatgcgcca gcccggagga ggacgcgctc tgctccttcg
     2701 acgaggactg cggcgacacc gcgctctatt tctgcgacca gcagaccggc cagtgccgcg
     2761 aaaaggcggg catgaccgac accgacgaca tcggcggcgg ggacgaggaa aaggacgaaa
     2821 gcgacatgaa cgacatagag gacggggacg aaaccacaga gtcaggtctg ccggacgaga
     2881 ccgttgacga gaccggctct gatgctcttc ccgacaacga caccccgtcc cagtggctcg
     2941 atccggtcac ggggctgacc atcgacatcg agatagacca actgaccctc gcgtggcaga
     3001 acccgacgat ggcggggctc accgacatca aggtggtgcg taacatgatc gccttcccgc
     3061 agaacatcta cgacggcgac ctgttgtacg aagggctcga ccagacctac gccgacacct
     3121 cggtcgactc gggcaaaagc cacttttaca gcgtgttcgc ctgctacggc gaactcgggt
     3181 gcagcgaacc ggccgtgatg ggcggcatcc cctgctacga ccggatggac ctcgtgttcg
     3241 tcatggatgt ctcgacgagc atggactata tcctctccga cctcgagaac gagatcggcg
     3301 aggtctggaa cgccgtgtcg cagaagatag agacgacgcc gcgcatgggg ctcaccgtat
     3361 tcgtggatga tgtgaaagtg gtcaacgacg gctccacccc ctacgcgacc gtcgaacaga
     3421 tcaagggcga tttccattcg tggtaccagc acacctccag caacgagcag acgcagtcga
     3481 gcgccaccaa ctacgactgg cccgagaact cgctcgacgg actcgcgctg accgccaaga
     3541 acttccagtg gcgcgatccc gccaaggcac tgcgcatcat catcctcgcc accgacgaca
     3601 ccttccgcga aaaaccggct tccttctcga gcggcatcgc ggcccagcat acctacgatg
     3661 aaacggtcgc cctcctgcag gagggccgca tccgcgttgc cgccttcgcc gcgaagctcg
     3721 gaggatcgac cggcaccgcc gatgtccagc ccgggttctt caccacctat aacggcaagg
     3781 aaccgatccc ggcagcgacg ggcggcgagg tcttcttcat agacgatgtg aagaacggga
     3841 cgctgtcgct tgtcgacgcg ataacgggat tcatcgagac cgaacgctgc gtcgccggcg
     3901 aacgctagcg acggggaccg gacggtgaac cgcttcatcg ccgcactgct cgacgaggcg
     3961 atcgggcgtt tcgaggagac ccgttggaag gccgacctgc tcctcgcggc cctgttccgt
     4021 caacgccaca tcggcaaacg cgatcgagcc ctcctgcgcg aacttttctt tacctacctc
     4081 cgccgccgct atctcgtcga gagtttcatg cgcgccggac tcacccgccc cgacgcggtg
     4141 gaacgggcgc aggggacgac cggcgtatcg ggcgacctgt tccactcctt tccgccgatc
     4201 taccgcgacg ccgcggtggc gcggtacggc gaaggggccg acgcggcgct cgcgtggctc
     4261 aacgggcgcg ccccgacgac gatacgcgcc aatgttcgcc gcgccggacg tgacgaactg
     4321 agaaaacgcc tcgccaccga gggtatccgg tgccttccga cgccggtcgc accgaacggg
     4381 ctcatcgtga ccgacggagc gtcgcaactg acccgctcgc ccctctacga aaaggggttc
     4441 ttcgaaatgc aggacgaatc gtcgcagatc gcggtcgaac tcgtccccgg cggtgcgaaa
     4501 accctgctcg acctttgcgc gg
//
LOCUS       DAOQUE010000213         4520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_21365_length_4520_cov_4.073908, whole genome shotgun sequence.
ACCESSION   DAOQUE010000213 DAOQUE010000000
VERSION     DAOQUE010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21365_length_4520_cov_4.073908"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKH10_07450"
     CDS             complement(<1..743)
                     /locus_tag="PKH10_07450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT27997.1"
                     /translation="MSRSLLWRILMVLMPILLAGFYFFLLLYQAERVAITDDARFYMD
                     AARSYGDYLEAGWNGFKYFDRGFIDRYWQNNHEHPSFAKLLMAGGYLVHKKFGILRET
                     VAYRVGISALAAVLLLFLFSFVRRAVSFRAAVFAALFFMFLPRTFFHARVATLDFTVG
                     ATCFIFVYCYWRGLTSRSWAWMTGVAFGFALASKLNAPFMVLPVLVHYCVVKWGDLKR
                     WPVRTIFAPQFVSMLLLSLPVFFALWPWL"
     gene            complement(740..1633)
                     /locus_tag="PKH10_07455"
     CDS             complement(740..1633)
                     /locus_tag="PKH10_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT27998.1"
                     /translation="MKFSSRTPYNIAFVFLMLTAIWSVFSFFSYHTAPLPERMPGPLL
                     DILSREVRPGEPIYLADQRFDLFVGSHPQYNIFPGGGSNAPKAARAARFFLVSGASMP
                     DDFDDIEGFSVTVAAEAEGMTLWRFDRTDGALAEYLLSDAFPEGLTIRSSAFPDGARY
                     RNGEFVIGPMAWERAQIKSGEFGPARRTALSVHPLDGADKWIELTVDPARWRAEVILL
                     GYGIASSGDCKGQCPPVTVTVTQGERTRSFETRDMVWQQAELREFAADLPLTIRIVAE
                     KAGKRHFYCDLIFRTAGEVTP"
     gene            complement(1630..2904)
                     /gene="serS"
                     /locus_tag="PKH10_07460"
     CDS             complement(1630..2904)
                     /gene="serS"
                     /locus_tag="PKH10_07460"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNT27999.1"
                     /translation="MFDGRLIMEQKDRVLASMHKRNFKDIHLIEAAAELIERRKRIIT
                     ESETARAQQNKRSKDMPQVMKSGTPEEKKAIQEDLKALAEQVKAFQPRVKAVEEELDA
                     VLLAIPNLLADDAPEGGEENAVEVSVWGEKPQFDFTPKEHDDLAKGLFDPERGAKLAG
                     ARFSVLWGKLATLERVLIQFMLDIQTRERGYTEVLTPLLVNRATMTATGQLPKFEEDL
                     FKTNDDLFLIPTAEVPVTNLHRDEILREEDLPRSYCAYTPCFRREAGSYGKDTKGYIR
                     QHQFNKVELVKFTRPEDSDAELMKLLRDAEEILRRLGLHYRVVRLAAQDQGFSSSKTF
                     DIEVWLPGQGRYREISSCSSFTDYQARRGNIRFKRAGAKATELCHTLNGSGLAIGRTL
                     IAIVENYQRADGTIVVPEALRPYMGCEVITPA"
     gene            3051..3509
                     /locus_tag="PKH10_07465"
     CDS             3051..3509
                     /locus_tag="PKH10_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNT28000.1"
                     /translation="MHQKLLLHLLLHSGKLLEERIKRALRPYGIHQGQARILNALNCY
                     GERSQIEIARGLHIERATATIMLQKLETAGLIKRTAHDRDGRVIVVSLTPKGKKAAEK
                     VDAVWQEIEEKLLATLGDLPRDTMEDALLTIRNAFGGKSPAFKITKEEGQ"
     gene            3506..>4520
                     /locus_tag="PKH10_07470"
     CDS             3506..>4520
                     /locus_tag="PKH10_07470"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT28001.1"
                     /translation="MRKMLVCFLASLVLWSVSCDNGTQRVPTDETALNDVDTPLADEL
                     VADDYTDAPITETGSDADEVAVVLPYPIVDTGQTHCYDDHTGIICPSTGAAYFGQDAQ
                     YIGNPPSYTVSNDGLTVLDNVTGLTWQRSPDTNGDGIIDASDKLTHEEALAWPATVNA
                     ASYGGFNDWRLPTIKEQYSLINFNGQDPSGLMSNDTSSITPFIDDTVFGFAYGDTAAG
                     ERIIDSQYASSNLYVDTTTVGDLLFGVNFADGRIKGYGLTMPNGDKKFFFVTLVRGNP
                     DYGINDFEDNDDGTITDHATGLMWMKDDSGEGMNWAAALAYAQTKNAESYLGYNDWRL
                     PHVK"
BASE COUNT          961 a         1345 c         1400 g          814 t
ORIGIN      
        1 cacagccacg gccacaacgc gaagaatacg ggtagtgaca ataggagcat cgacacgaac
       61 tgcggcgcga atatcgtgcg gaccggccac cgtttgaggt caccccactt cacgacgcag
      121 tagtgtacaa gcaccggcag gaccatgaaa ggcgcgttca gtttcgaggc gagcgcgaag
      181 ccgaaggcga ccccggtcat ccacgcccag gaccgcgagg tgagcccgcg ccagtagcag
      241 tagacgaata taaagcaggt cgccccgacg gtgaagtcga gcgtcgccac gcgggcgtgg
      301 aaaaaggttc gcggcaggaa catgaagaag agcgccgcga acacggcggc ccggaacgaa
      361 acggcgcgac ggacaaaact gaagaggaac agcaggagta cggcggcgag agcgctgatg
      421 ccgacgcgat aggcgaccgt ctcgcgcagg atgccgaatt tcttgtggac aagatagccg
      481 cccgccatca gcaatttcgc gaacgaggga tgttcgtgat tgttttgcca gtaacggtcg
      541 atgaagcccc ggtcgaagta cttgaaaccg ttccagcccg cttcgaggta gtcgccgtaa
      601 cttctcgccg cgtccatata gaaccgcgca tcgtcggtga tggcgacccg ctcggcctga
      661 tagaggagca ggaagaaata gaacccggcg agcagtatcg gcatcaatac catgaggata
      721 cgccagagga gcgaccggct catggcgtca cctccccggc ggtccggaat atcaggtcgc
      781 agtagaaatg gcgtttgccc gccttttccg caacgatgcg tatcgtgagc ggcaggtcgg
      841 cggcgaattc ccgcagttcg gcctgttgcc agaccatgtc acgggtctcg aaggagcgcg
      901 tccgctcacc ttgcgtcacc gtcaccgtca ccggcgggca ctgtcccttg cagtcgcccg
      961 aggaggcgat gccgtaaccg agcaggatca cctcggcgcg ccagcgggcc ggatcgacgg
     1021 tcagttctat ccatttgtcg gcgccgtcga gcgggtggac cgagagggcg gtgcgacgcg
     1081 ccggaccgaa ctcgccgctt ttgatctgcg cccgctccca tgccatcggg ccgatcacga
     1141 actcgccgtt gcgatagcgg gcaccgtcgg gaaaggccga cgagcggatc gtgagcccct
     1201 ccgggaaggc atcggacagc agatattcgg ccaaagcgcc gtcggtgcga tcgaagcgcc
     1261 acagcgtcat tccctcggcc tccgcagcga ccgtgacgga aaagccctcg atatcatcga
     1321 aatcgtcggg catcgaggcg ccgctgacga ggaagaaacg ggcggcacgg gccgctttcg
     1381 gggcgttcga cccgccaccc gggaagatgt tgtattgcgg gtggctcccg acgaacaggt
     1441 cgaaccgctg gtcggcaaga tagatcggct caccgggacg cacctcgcgc gaaaggatgt
     1501 caaggagcgg gccaggcatc cgttccggaa gaggcgcggt atgataggag aagaaactga
     1561 agacgctcca gatcgccgtc agcatcagga atacgaacgc gatgttgtag ggtgttcggg
     1621 aagagaactt catgcggggg tgatgacctc gcagcccata tacggacgaa gcgcttcggg
     1681 cacgactatg gttccatcgg cgcgctggta attctcgacg atcgcgatga gtgtccggcc
     1741 gatggcgagc cccgaaccgt tgagcgtgtg gcagagttcg gtcgcctttg ctcccgcccg
     1801 tttgaagcgg atattgccac gccgcgcctg atagtcggtg aaggaactgc acgacgatat
     1861 ctcgcggtac cgcccctgtc cggggagcca cacctctatg tcgaaggtct tggacgacga
     1921 gaagccctga tcctgagcgg cgagccgcac gacgcggtaa tgaagcccca ggcggcgcag
     1981 tatctcctcg gcgtcgcgaa gcagtttcat aagttcggcg tccgaatcct cgggccgcgt
     2041 gaacttgacg agttcgacct tattgaactg gtgctggcgg atgtagccct tggtgtcctt
     2101 gccgtaactc cccgcctcgc gccggaaaca cggggtgtag gcgcagtagg aacgcggcag
     2161 gtcctcctcg cgaaggatct cgtcgcggtg gaggttggtc accggcacct cggcggtcgg
     2221 tatcaggaaa agatcgtcgt tcgtcttgaa caggtcctct tcgaacttgg ggagttgccc
     2281 ggtcgccgtc atcgttgcgc ggttgaccag aagcggcgtc agcacttcgg tatagccccg
     2341 ttcgcgggtc tggatgtcga gcatgaactg gatgagcact cgttcgagcg tcgccagttt
     2401 tccccacagc acgctgaacc gcgcgccggc aagtttcgct ccccgctcgg ggtcgaacag
     2461 ccctttggcg agatcgtcgt gctccttcgg ggtgaagtcg aactgcggct tctcgcccca
     2521 taccgacacc tcgacggcgt tctcctcgcc cccttcgggc gcgtcgtcgg cgagaagatt
     2581 cggtatcgcg aggagcaccg cgtcgagttc ctcttcgacc gccttgacac ggggctggaa
     2641 cgccttcacc tgctcggcga gcgccttaag atcctcctgt atcgccttct tctcctcggg
     2701 ggtgccgctc ttcatgacct gcggcatatc cttggaccgt ttgttctgct gggcgcgggc
     2761 cgtttccgat tcggtgatga tccgtttgcg ccgctcgatc agttcggccg ccgcttctat
     2821 cagatggata tccttgaagt tccgcttgtg catcgaagcc agaacgcgat ccttctgctc
     2881 catgatgagt ctgccgtcga acatcgccgt tctcccttaa agttcataga aataacgggc
     2941 gcttcatcat agtgggcggg gggttctttg caagttttta taaggcaagg cattttcttg
     3001 acaaacaaca agagcgccga tattgttagc gggctaacaa tcgaggtgcg atgcaccaga
     3061 aacttcttct tcacctgctg ctgcacagcg gcaaactgtt ggaagaacgg ataaaacggg
     3121 cgctgcgtcc ctacggcatc catcaggggc aggcgcggat actcaatgcg ctgaactgtt
     3181 acggcgagcg gtcgcagatc gaaatcgccc ggggattaca catcgagcgg gcgaccgcga
     3241 cgatcatgct ccagaaactc gaaacggccg ggttgataaa aaggacggca catgaccggg
     3301 acgggcgggt catcgtcgtt tctctgaccc cgaaaggaaa gaaggccgcc gaaaaggtgg
     3361 acgcggtgtg gcaggagatc gaagagaaac tgctggccac cctcggcgac ctgccgcgtg
     3421 acacgatgga agatgctctg cttacgatac gaaacgcctt cggaggaaaa agtcccgcgt
     3481 tcaaaataac caaggaggaa ggacaatgag aaagatgctc gtgtgttttt tggcatctct
     3541 ggtgctctgg tcggtctcat gcgacaacgg cacgcaacgg gtccccaccg atgagacggc
     3601 cttgaatgat gtcgatacgc cgcttgccga cgaactcgtg gccgatgact atacggacgc
     3661 cccgatcacc gagaccggaa gcgacgccga tgaagtcgcc gtcgttctac cgtatccgat
     3721 cgtcgatacc gggcaaactc actgctacga cgaccatacc gggatcattt gcccgagcac
     3781 gggggcggca tatttcggtc aggatgctca gtatatcggc aacccgccga gctacacggt
     3841 cagcaacgac ggactgacag ttcttgataa tgtcaccgga ttgacctggc aacgcagccc
     3901 ggacacgaat ggagacggca tcatcgacgc tagcgacaaa ctgacgcatg aggaggcgct
     3961 cgcatggccc gcgaccgtca acgccgcgag ctatggcggt ttcaacgact ggcgcctgcc
     4021 gacgatcaag gagcagtact cgcttatcaa cttcaacgga caggatccga gcggcctcat
     4081 gagcaacgac acctcgtcca tcacgccgtt cattgacgac acggtattcg gctttgcgta
     4141 cggcgacacg gcggcgggcg agcggatcat cgactcgcag tacgcctcga gcaacctgta
     4201 tgtggacacc acgactgtcg gggatctcct gttcggcgtc aacttcgccg acggccgcat
     4261 caagggatac ggcctcacaa tgccgaacgg ggacaagaag ttcttcttcg tcacgctcgt
     4321 tcgcggcaac cccgactacg gcatcaacga ttttgaggac aacgatgacg gcacgatcac
     4381 cgaccacgcc accggcctca tgtggatgaa ggacgacagc ggcgagggga tgaactgggc
     4441 agcggcgctc gcctacgctc agacaaaaaa cgccgagagc tacctcggct acaacgactg
     4501 gcgcctgccg catgtcaagg
//
LOCUS       DAOQUE010000214         4507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_21442_length_4507_cov_5.377583, whole genome shotgun sequence.
ACCESSION   DAOQUE010000214 DAOQUE010000000
VERSION     DAOQUE010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21442_length_4507_cov_5.377583"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4492)
                     /locus_tag="PKH10_07475"
     CDS             complement(<1..4492)
                     /locus_tag="PKH10_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNT28002.1"
                     /translation="MAPRAGEGTKVELRTLRIVQGGSSIEMAGGAASLGGCMDAARVG
                     GDGECLRRLERRSGGVTEWFDNTESGIEQGFDIAEPLSDDGGTLTVEMQVVAGSARGE
                     GGKVTLLSDGTAVASLHSLFIVDAKGAKREGTITAQGKTIFLTVEVTGLSYPLTIDPL
                     YSGTPDRTLEVDQAGAAFGSPVASAGDVNGDGYGDVIVGASMYDNGQSNEGVAFVYHG
                     SATGISTTYARLLEVNSGAAYFGSSVSGAGDVNGDGYGDVIVGAPYYDNGQDNEGAVF
                     IYHGSASGIGATAVRTLESGQAEAHFGMAVSGAGDVNGDGYSDIIVGARDYDDGQADE
                     GAAYIYHGSASGIGASYARLLQSDQTDAYFGFSVSGAGDVNGDGYSDVVIGARNYQSG
                     AGQTDEGAIYIYHGSSSGIGTSYARMIQAEQISAYLGDSVAGAGDVNGDGYSDIIAGA
                     PGYDNGQTDEGVAFVYHGSSSGIGTTYNTILQSDQADAQMGGSVAGAGDTNGDGYADV
                     VVGANYYDFGQTNEGRAFIYHGSATGINTTAAVTVESDQTNAQLGGSVAGAGDVNGDG
                     YSDIIVGAAGYDGGQTDEGAAFVYHGAASGIGTAYNQLLEVDQASASFGYSVAGAGDV
                     NGDGYGDIIIGAYKYDNGETDEGAAFIYHGSASGIGTAYARRLESDQASVFFGYSVAG
                     AGDVNGDGYGDIIVGSYFYDDAEGAAFIYHGSAAGIGASYNARIEENLALDQFGSSVA
                     GAGDVNGDGFGDIIVGAPYYTNGNTNEGAIYIYHGSSGGINTTYARRIESNVVWAEFG
                     SSVASAGDVNGDGYSDVIVGAHKYSNGHSEEGAIYIYHGSATGIGTTHDQRIESNLAD
                     AWFGHSVSGAGDVNGDGYGDIVAGAPRYDNGQTNEGVVLLYHGSASGIGTSYAVRLEQ
                     NQADLLFGWSVAGAGDVNGDGYADIIIGTPWYDSAQTNEGAAYIYHGSSSGIVGAHVR
                     RLDPLNQTGAYFGWSVAGAGDVNGDGYSDILVGAQWYDQGQSDEGAAFVYHGNNGEAL
                     RMTGQQKKGTTAVSPQGKIGDDDTLTLSVFARSFMGRSKAKMLYEVKAHGAPFNYTGF
                     QRAGAWTDTTLAGTTLTTGIDLDKALYHWRVRYQFMDGSMSRWFVFDRSAGVYTPDLR
                     VLKGQGETCSAGSECGSGFCADGFCCDLACGGSDTTDCQACSVTAGASVNGTCKVLGS
                     AHTCRDAVDVCDAAEVCNGFDTACPENVFAPTGTPCASDDLYCTGDETCTGGSCGSEG
                     YPCETDYFCDEDIDDCINERQAPCDTNNDNPANSHDTIANVTITYTTAGGWTAPADCA
                     WTCDTNYAEEGGLCINSKEVPCDTDNGNPAHSTDTIANVTITYTTAGGWTSPADCAWT
                     CDTDYILEGGLCINSKEVPCDTNNDNPANSSDTIANVTITYTSAGGWTAPADCAWTCD
                     TNYAEEGGLCINSKEVPCDTDNGNPAHSTDTIANVTITYTTAGGWTAPADCAWTCDTN
                     YAE"
BASE COUNT          767 a         1511 c         1326 g          903 t
ORIGIN      
        1 cctcggcgta gttcgtgtcg caggtccacg cacagtcggc gggagcggtc cagccacccg
       61 ccgtcgtgta ggtgatcgtc acattcgcga tggtgtcggt gctgtgcgcc ggattgccgt
      121 tgtcggtgtc acatggcacc tccttcgagt tgatacagag gccaccctcc tcggcgtagt
      181 tcgtgtcgca ggtccacgca cagtcggcgg gagcggtcca gccgcccgcc gaggtatagg
      241 tgatcgtcac attcgcgatg gtgtcgctgc tgttggccgg gttgtcattg ttggtgtcgc
      301 acggcacttc cttcgagttg atgcagagac cgccttcgag gatatagtcg gtgtcgcagg
      361 tccacgcaca gtcggccggt gaggtccagc cgccggccgt cgtgtaggtg atcgtcacat
      421 tcgcgatggt gtcggtgctg tgcgccggat tgccgttgtc ggtgtcacat ggcacctcct
      481 tcgagttgat acagaggcca ccctcctcgg cgtagttcgt gtcgcaggtc cacgcacagt
      541 cggcgggagc ggtccagccg ccggcggtcg tgtaggttat cgtcacattc gcgatggtgt
      601 catggctatt cgccgggtta tcattattgg tgtcgcatgg cgcctgtctt tcattgatac
      661 agtcgtcgat atcctcgtcg cagaaatagt cggtctcgca gggatatcct tccgatccgc
      721 atgagccccc cgtgcaggtc tcgtcgccgg tgcagtagag gtcgtcgctg gcgcaagggg
      781 tcccggtggg ggcgaaaaca ttctcgggac aggcggtatc aaagccgttg cagacctcgg
      841 cggcgtcaca gacatctacg gcgtcgcggc aggtgtgcgc gctaccgagc accttgcagg
      901 tcccgttcac gcttgctccc gccgtcacgc tgcatgcctg acagtcggtg gtatcgctgc
      961 cgccgcaggc cagatcacag cagaacccgt cggcgcagaa accgctaccg cactcgctgc
     1021 ccgccgagca ggtctcccct tgtcccttca gcacgcgcag gtcgggcgta tagacgccgg
     1081 cgctccggtc gaacacgaac caacgcgaca tagagccgtc catgaactgg taccgcaccc
     1141 gccagtgata cagcgccttg tcaagatcga tgccggtggt cagcgtggtc cccgccagcg
     1201 tcgtatcggt ccacgcgcct gccctctgga agccggtgta gttgaagggc gccccgtgcg
     1261 ccttcacctc gtagagcatc ttcgctttgc tcctccccat gaaggagcgg gcgaacaccg
     1321 acagggtcag cgtgtcgtcg tccccgatct ttccctgcgg agagacggct gtcgttcctt
     1381 tcttctgctg tcccgtcatc cggagcgctt cgccgttgtt gccgtggtac acgaaggcgg
     1441 caccttcatc gctctggcct tgatcatacc actgagcccc gacaaggata tcgctgtagc
     1501 cgtcgccatt cacatcgccc gccccggcga ccgaccagcc gaaataagcc cccgtttgat
     1561 tcagcgggtc gagtctccgg acatgggccc ctacgatacc gctcgacgaa ccatggtaaa
     1621 tgtaggcggc cccttcgttg gtctgcgcgc tgtcatacca cggggtgccg atgatgatat
     1681 cggcgtaacc gtcgccgttc acatctcccg cccccgcgac cgaccagccg aaaagcaggt
     1741 ccgcttggtt ttgttcgagt ctgacggcat aggatgtgcc gatgccgctc gccgaaccgt
     1801 ggtagaggag gacgacccct tcattggtct gaccattgtc gtagcgaggt gcaccggcga
     1861 cgatgtcgcc gtagccatcg ccattcacat cgcccgcgcc ggagaccgaa tggccaaacc
     1921 aggcgtccgc cagatttgat tcgattctct ggtcgtgggt cgtgccgata ccggtcgccg
     1981 atccatggta gatatagatg gctccttcct cgctgtggcc gttggaatat ttatgcgcgc
     2041 cgacgatgac atcgctgtaa ccgtcgccgt tcacatcgcc cgccgaggcg accgatgacc
     2101 caaactcggc ccacaccaca ttcgactcga tcctgcgggc ataagtcgtg ttgatgccgc
     2161 ccgaagaacc atggtagatg taaatggctc cttcgttggt atttccgttg gtgtagtaag
     2221 gagccccgac gatgatgtcg ccgaagccat cgccgttcac atcgccggcg ccggcgaccg
     2281 aagagccgaa ttgatcgagc gccagattct cttcaattct ggcgttgtaa ctcgcaccga
     2341 tgccggccgc cgagccgtgg tagatgaacg cggccccctc ggcgtcgtcg taaaaatagg
     2401 acccgacgat gatgtccccg tatccgtcgc cgttcacatc gcccgctccg gcgaccgagt
     2461 agccgaaaaa gacgctggcc tgatccgatt cgagtctccg ggcataggcc gtcccgatac
     2521 cgctcgccga tccgtggtag atgaacgcgg ccccctcgtc ggtctcaccg ttgtcgtatt
     2581 tgtatgcccc gatgatgata tcgccatagc cgtcgccatt cacatcgccc gcgccggcga
     2641 ccgagtagcc gaaagaggcg ctcgcctgat ccacctcgag cagttggtta taggccgtcc
     2701 cgatgccgct cgccgcgcca tgatacacga acgcggcacc ttcgtcggtc tgtccgccat
     2761 cgtaaccggc tgccccgacg atgatgtcgc tgtagccgtc gccgttcaca tcgcccgctc
     2821 cggcgaccga gccgcctaac tgggcattcg tctgatccga ctcgacggtg acggcggcgg
     2881 tcgtgttgat gccggtcgct gaaccgtggt agatgaaagc gcgtccctcg ttggtctggc
     2941 caaaatcgta atagttcgcc ccgacgacga catcggcgta cccgtcgccg ttcgtatcgc
     3001 ctgcgccggc cacggaaccg cccatttggg cgtccgcctg atctgattgg aggatggtgt
     3061 tgtaggtcgt tccgatgccg ctggaggacc cgtgatacac gaaggcgacc ccttcgtcgg
     3121 tctgtccgtt gtcatagccc ggtgcgccgg cgatgatgtc gctgtagccg tcgccgttca
     3181 catcgcctgc tccggcgacc gaatcgccga gatacgctga tatctgctct gcctggatca
     3241 tgcgggcgta agaggtcccg ataccggagg aggaaccgtg atagatatag atggcccctt
     3301 catcggtttg gccggcgccg ctttgatagt tccgcgcccc gatgacgaca tcgctgtagc
     3361 cgtcgccgtt cacatcgccc gcgcccgaga ccgagaagcc aaagtaggca tctgtctgat
     3421 ccgattggag caggcgggca taggaggcac cgatgccgct cgccgatccg tggtaaatat
     3481 aagcggcccc ttcatcggcc tgcccgtcgt catagtcgcg cgctccgacg atgatatccg
     3541 agtacccatc gccgttcaca tcgcccgcac ccgaaaccgc catcccgaaa tgagcctccg
     3601 cctgacccga ttcgagagtg cggacagcgg tcgcaccgat accactcgcc gaaccatgat
     3661 agatgaacac ggccccttcg ttgtcctgcc cgttgtcata gtagggtgcc ccgacgatga
     3721 catcgccgta tccgtcgcca ttcacgtcgc ccgcgccaga gaccgaacta ccgaaatagg
     3781 ctgccccgga attcacttcc aacagacggg cataggtcgt cgagatgccg gtcgccgaac
     3841 cgtgatacac gaacgcgacc ccttcgttgc tctgcccgtt gtcgtacatg gaggcgccga
     3901 cgatgacatc gccgtagcca tcgccgttca catcgcccgc agaggcgacc ggcgaaccga
     3961 aggcggcgcc ggcctgatcc acctccagtg tccgatccgg cgtgccgctg tagagggggt
     4021 ctatcgtgag cgggtaggaa agccccgtca cctcgacggt cagaaaaatg gtctttccct
     4081 gcgctgtgat ggtcccttcg cgcttcgctc ccttcgcgtc gacgatgaac aggctatgca
     4141 gggaggccac cgccgtgccg tccgacagga gcgtcacctt ccccccttcg ccgcgcgcgc
     4201 ttcccgcgac gacctgcatc tcgaccgtca gcgtcccgcc atcgtccgag agaggctcgg
     4261 cgatatcgaa cccctgttct atgccgcttt cagtattgtc aaaccattcc gtcaccccgc
     4321 cggagcgccg ctccagccga cggagacatt cgccgtcgcc gccgacccgt gccgcgtcca
     4381 tgcacccgcc gagcgatgcc gctccgcccg ccatctcgat cgacgagcca ccctgcacga
     4441 tgcggagcgt ccgcagttcc actttcgtcc cctcccccgc gcgcggcgcc accgagaagc
     4501 gtccctc
//
LOCUS       DAOQUE010000215         4496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_21514_length_4496_cov_3.593560, whole genome shotgun sequence.
ACCESSION   DAOQUE010000215 DAOQUE010000000
VERSION     DAOQUE010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21514_length_4496_cov_3.593560"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="PKH10_07480"
     CDS             <1..1276
                     /locus_tag="PKH10_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT28003.1"
                     /translation="TGDITASLGLFEWVDLGLAVPVVLFQNGDGWNGGEKLPPATLGD
                     IRFAPRARIYRTEDRFFSIAVVPVLAFPTGQFLHELSGAAGFTFSPTLALSIRSKWVC
                     GGLNFLYRMTQYDHVIGATHIADQFGIKAQVTGYAIPETLSFSGEWSFASTASKTFGE
                     KDESPMEAALAASWHAPHDLTLTVGGGAGIMSGFATPLFRVFAGLTWILEQEKETTPP
                     EKPAVEETPAAEKKPATAPSPVPVTEKKPEPAPQQVTPKKPMVVELGAKPEPKKPEEK
                     KPVPPPQVEQKKTAPAVAPVPARPTPIPPLPVKKMEPRRGTQMTEVVFFQDNSTTFTD
                     ESRVKLEKINLLLSSNYTLKIRIEGHADRNEKPEMGLKRAQAIKLWLIDRDIESSRIQ
                     TMGIGADEPAASSDIEADRARNRRVTFWVLDK"
     gene            1324..2256
                     /locus_tag="PKH10_07485"
     CDS             1324..2256
                     /locus_tag="PKH10_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019275154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNT28004.1"
                     /translation="MSSQKFRLVTRSDFDGLVCGAIFKELDLIDEVKFVHPKDMQDGL
                     VEITGRDITTNLPYVEGVHLCFDHHVSETLRLTMRRQNHVIDPDAPSAARVVYNHYHG
                     KKTLPRITEEMMAAVDKADAAQFSINDILNSERWVTLNFLMDPRTGLGRFRDFRISNY
                     DLMILLIDLVRTKTIDEILGHPDVKERSDLYFEHQDLFRAMIQRAAKVHKNLIYIDLT
                     RETVIYAGNRFVPYALFPYCNISVTRMWGLKKEKVVFSVGKSIFNRTSKANIGEILLA
                     YGGGGHRNAGTCQIEIDRADAVEKELLEAFTQTA"
     gene            2338..3315
                     /locus_tag="PKH10_07490"
     CDS             2338..3315
                     /locus_tag="PKH10_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNT28005.1"
                     /translation="MEHAPLNERIERTATAIRALKNEIQKIIVGQEEMIEALVMGIIA
                     EGHILLEGVPGLAKTTAVRAFAAATDLDFKRIQFTPDLLPADIMGSRIFNMEKRDFEI
                     KQGVVFSNVILADEINRAPAKVQSALLEAMQERQVTIGEKTMRLPDPFIVLATQNPIE
                     QEGTYQLPEAQIDRFMFKVLIDYPTVDEELRIIERHEKREFDKALARIADRESIKEWR
                     SAVDLIYTDDRIKKYVVSLVFETRNPRKNDLKQLIEIGASPRASLYLVLGARIRALIE
                     GRGFVTPDDVKEVAYPVLRHRIIPSFEAEAEKLTTDRIIERVLNQVEVP"
     gene            3319..4182
                     /locus_tag="PKH10_07495"
     CDS             3319..4182
                     /locus_tag="PKH10_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNT28006.1"
                     /translation="MLQEELLKQIRLIDLKVRRQVESSLQGSYHTLFKGHGLEFQEVR
                     EYVEGEDVRLMDWNVTARMGRPFVRVYREERELTVMVVVDVSASTLFGRAWSGREVAA
                     TLGAALAFSAIRNNDKAGLILFSDRIEGYIPPRRGRNHLLTIIRALLTTPAKGRRTDP
                     DIPFRFINKAMKKKSLLFFLSDMQFGPLPDSAAVAARRHEIVPVAIFDRMSYAPERNA
                     FVRTRDPESGMARVLDLSVSAEGRWDHVRKEKEDAFKRIGSLPIWIENQAEFINDLVL
                     GFKKMQRKGAR"
     gene            4179..>4496
                     /locus_tag="PKH10_07500"
     CDS             4179..>4496
                     /locus_tag="PKH10_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28007.1"
                     /translation="MRRYLLIVLLPILLFAEDITPQLEKKEIFLGEPVILFVPLKPGK
                     ELSAFPKSDDANFALVKTEPAGERVQLTLSAIETGTLNTPPLILRIDGTIRSDVRLPR
                     DAVV"
BASE COUNT          986 a         1489 c         1275 g          746 t
ORIGIN      
        1 gaccggcgac atcaccgcct cgctcggcct attcgaatgg gtcgacctcg gactcgcggt
       61 cccggtcgtc ctcttccaga acggcgacgg gtggaacggc ggcgaaaaac tgccgcccgc
      121 gacgctcggc gacatccggt tcgccccccg cgcccgcatc taccggaccg aggaccggtt
      181 cttctcgatc gccgtcgtgc cggtacttgc attcccgacc gggcagttcc ttcacgaact
      241 ctccggcgcc gccggcttca ccttcagccc gacgctcgcc ctgtcgatac gatcgaaatg
      301 ggtctgcggc ggcctgaact tcctgtaccg tatgacgcaa tacgaccatg ttatcggcgc
      361 cacccacatc gccgatcagt tcggcatcaa ggcgcaggtg accggctacg cgataccgga
      421 gacgctctcc ttctcggggg aatggtcgtt cgcctcgacc gcctcgaaga ccttcggcga
      481 aaaggacgag tcgccgatgg aggcggccct cgcggcctcg tggcacgcgc cgcatgacct
      541 caccctgacc gtcggcggcg gcgccggcat catgagcgga ttcgccaccc ccctcttccg
      601 cgtgttcgcc ggtctcacct ggatactgga gcaggaaaaa gagaccacgc cgccggaaaa
      661 accggcggtc gaagagacac cggcggccga aaagaagccg gccaccgcac cgtcaccggt
      721 gccggtgacg gagaaaaaac cggaaccggc cccgcagcaa gtgaccccca agaaaccgat
      781 ggtggtcgaa ctgggcgcga aaccggagcc gaaaaaaccc gaggagaaga aaccggtgcc
      841 accgccgcag gtagagcaga aaaaaacggc gcccgccgtc gctcccgttc cggcgagacc
      901 gacaccgatc ccgcccttgc cggtcaagaa gatggaaccg agaagaggga cccagatgac
      961 cgaggtcgtc ttctttcagg acaacagcac caccttcacc gacgaatcgc gggtcaaact
     1021 cgagaagata aatctcctcc tttcctctaa ctacaccctc aagatacgca tcgaaggaca
     1081 cgccgaccgt aacgagaaac cggaaatggg cctcaaacgg gcgcaggcca taaaactctg
     1141 gctcatcgac cgcgacatcg agtcttcgcg catacagacg atggggatcg gcgccgacga
     1201 accggcggcg agcagcgaca tcgaggccga ccgggcgcgc aaccggcgag tcaccttctg
     1261 ggtgcttgat aagtgagaca cctttttacc ggagatatga ccatcactta aggaggagac
     1321 atcatgtcgt cccagaagtt ccgcttggtg acccgtagcg atttcgacgg gctggtgtgc
     1381 ggcgcgatat tcaaagaact tgacctcatc gacgaggtga agttcgtcca ccccaaggac
     1441 atgcaggacg gcctcgtcga gataacgggc cgcgacatca ccaccaacct cccctatgtg
     1501 gagggcgtgc acctctgctt cgaccaccat gtcagcgaga ccctccgtct gacgatgcgc
     1561 cgccagaacc atgtcatcga tccggacgcc ccctcggccg cccgcgtcgt ctataaccat
     1621 taccacggta aaaagaccct tccccgcatc accgaggaga tgatggcggc ggtcgacaag
     1681 gccgacgcgg cgcaattctc catcaacgac atactcaact ccgaacggtg ggtgacgctc
     1741 aatttcctca tggatccgcg taccgggctc ggccggttcc gcgatttccg catcagcaac
     1801 tacgacctta tgatcctgct catcgatctc gtccgcacca agaccatcga cgagatactc
     1861 ggccaccccg acgtcaagga acggagcgac ctctatttcg aacatcagga cctgttccgc
     1921 gccatgatac agcgcgccgc caaggtgcac aagaacctca tctacatcga tctcacccgc
     1981 gagacggtca tctacgcggg caaccgcttc gtcccctacg cgctgttccc ctactgcaac
     2041 atcagcgtga cccggatgtg gggactcaag aaggaaaagg tcgtgttcag cgtcggcaag
     2101 tcgatattca atcgcacctc caaggcgaac atcggcgaga tcctgctcgc ctacggggga
     2161 ggcggccacc gcaacgccgg cacctgccag atagagatcg accgcgccga cgccgtcgag
     2221 aaggaactgc tggaagcctt cacccagacc gcgtagtggt acgcgaccca caatgccggg
     2281 aataaaacag caccacacac ctgttcttgt gccgtcaccg atgtaaggag gtatatcatg
     2341 gagcacgccc cgctgaatga acggatcgag cggaccgcga ccgccatccg cgccctgaaa
     2401 aacgagatac agaagatcat cgtggggcag gaggagatga tagaggcgct cgtcatgggc
     2461 atcatcgccg aggggcatat cctcctcgaa ggggtaccgg ggctcgccaa aacgaccgcc
     2521 gtccgggcct tcgccgccgc gaccgatctc gatttcaagc ggatccagtt cacccccgat
     2581 ctgctccccg ccgacatcat ggggagccgc atcttcaaca tggagaaacg ggacttcgag
     2641 ataaaacaag gcgtcgtgtt cagcaatgtc atcctcgccg acgagataaa ccgcgccccc
     2701 gcgaaggtcc agtcggcgct cctcgaggcg atgcaggagc ggcaggtcac catcggcgag
     2761 aagacgatga gactgcccga tcccttcatc gtgctcgcga cgcagaaccc catcgaacag
     2821 gaggggacct accagctccc cgaggcccag atagaccgtt tcatgttcaa ggtgctcatc
     2881 gactacccca ccgtcgacga ggaactgcgc atcatcgagc ggcacgagaa acgagagttc
     2941 gacaaagcgc tggcccggat cgccgaccgc gagtccatca aggagtggcg ctcggcggtc
     3001 gacctcatct acactgacga tcgcatcaag aagtatgtgg tgtcactggt attcgagacc
     3061 cgaaaccctc gcaagaacga cctgaagcaa ctcatcgaga tcggcgccag cccgcgcgcc
     3121 agcctctatc tcgttctcgg cgcccgcatc cgcgctctca tcgagggacg cggcttcgtg
     3181 acccccgacg atgtcaagga ggtcgcctat ccggtcctgc ggcaccgcat catacccagt
     3241 ttcgaggccg aagcggagaa actcacgacc gaccggatca tcgagcgggt gctcaatcag
     3301 gtggaagtcc cctgagccat gttgcaggaa gagctcctca aacagatacg gctcatcgac
     3361 ctcaaggtgc gtaggcaggt cgaatcgtcc ctgcaggggt cctaccacac cctgttcaag
     3421 gggcacggcc tcgaatttca ggaggtccgc gagtatgtcg agggagagga tgtccgactg
     3481 atggactgga atgtcacggc gcgcatgggc cggcccttcg tccgggtcta ccgcgaagag
     3541 cgggaactca ccgtgatggt cgtggtggat gtctccgcct ccacattgtt cggtcgcgcg
     3601 tggtcgggcc gcgaggtcgc agcaacgctc ggcgccgcac ttgccttctc ggcgatacgg
     3661 aacaacgata aggcggggct gatactattc agcgaccgca ttgagggata catcccgcca
     3721 cggcgcggcc gcaaccacct gctcaccatc atccgcgccc ttctcaccac gccggccaaa
     3781 gggcggcgga ccgaccccga catccccttc cggttcatta acaaggcgat gaagaagaaa
     3841 tcgctcctct tcttcctttc cgacatgcag ttcggccccc ttcccgatag cgccgccgtt
     3901 gcggcgcgtc gccatgagat cgtgccggtc gccatcttcg accggatgag ttacgcaccc
     3961 gaacgcaacg ccttcgtccg tacccgcgac cccgaaagcg gcatggcgcg ggtgctcgac
     4021 ctttcggtga gcgccgaggg tcggtgggac cacgtcagaa aagaaaagga agatgccttc
     4081 aaacggatag gttccctgcc gatatggatc gagaatcagg ccgaattcat taacgatctc
     4141 gttctcggct tcaaaaagat gcaacgaaag ggggcgcgat gaggcgatat ctgctcatcg
     4201 ttcttcttcc cattcttctg ttcgccgagg atatcacgcc gcagttggag aaaaaggaga
     4261 tcttcctcgg cgaaccggtc atactcttcg tgccgctcaa gccggggaaa gagctctcgg
     4321 ccttccccaa gagcgacgat gccaacttcg cccttgtcaa gaccgagccg gcaggcgaac
     4381 gggtgcaact cactctctcg gcgatcgaga ccggcacgct gaacacccct cccctcatcc
     4441 tgcgcatcga cgggaccatc cgctcggacg tgcggctgcc tcgcgatgcc gtggtg
//
LOCUS       DAOQUE010000216         4480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_21636_length_4480_cov_4.663503, whole genome shotgun sequence.
ACCESSION   DAOQUE010000216 DAOQUE010000000
VERSION     DAOQUE010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21636_length_4480_cov_4.663503"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PKH10_07505"
     CDS             complement(<1..590)
                     /locus_tag="PKH10_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28008.1"
                     /translation="MYAERSFFAIAVRAAILLIPLMFLLGCGKASYPAADADATTFSD
                     TDLTDTGTLLSDEDDPVAEEEPLPDDERSDDLTDTALLSDETGDDLLSDADAPEGHRI
                     FVWTLMHAASEEVEEGMFDGRNYPAVTPFEGRLWLFGGEKKRDAWRSDDGVSWTLMAT
                     EVAPSFDGNRSVVVKDDLLWTVDTAGVWNSADGVEWS"
     gene            729..1928
                     /locus_tag="PKH10_07510"
     CDS             729..1928
                     /locus_tag="PKH10_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT28009.1"
                     /translation="MKDRFGAIVSFLGLKRSIVGLLTMVVLVGLGEKMAERFLPLYLI
                     ALGASPLIPGLLNGLDNLLSALYSYPGGWLTEKLGYKRALAVFNLVAMGGFLIVIAFP
                     HWIAVIVGSVFFLSWTALSLPATMELVSAVLPKNKRTMGVSVHSLVRRIPMALGPVIG
                     GVLIDLYGVTTGVRLSFTVAFILGAVSLVVQQGMIEERREEKEPATGGLRSFIALMPK
                     ELKALLASDILIRFCEQMPYAYLAIWAMQSPQGARVSGTEFGILTAIEMVVALLIYVP
                     VAYLADKGEKKPFVLITFFNFTIFPVILLFSTGFTMLVVAFVVRGLKEFGEPTRKALI
                     MDLAPEGRKAATFGSYYLVRDTVVSLAAFAGAWLWQVSPAVNLLTAAACGALGTILFA
                     VTGKERV"
     gene            1998..2807
                     /locus_tag="PKH10_07515"
     CDS             1998..2807
                     /locus_tag="PKH10_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28010.1"
                     /translation="MKSSVVFMLCLLSMLFIACGGEENDPVDSEPVNDDATTPDNDTA
                     GTGTLVFAGVANGTAPMTGIVGLKEVIAASQTNLMHGTGLELWVTEISVSQGLVADGV
                     ADTFVWEKIGAGDAIKPIPEYSFTHDSLPIGEYQSIKMVFKNSIVMTAVYHDDQTTEI
                     EMPSSLDQGSCGDETLITQYFSPNGNHALEGGVFHVQSAGEKSSAFTISEGKTTTIKW
                     QLGKPGVQLTDCSFEWVDANGNGSWDCGVDSAQNAQCSVPNVMFYFTVEEE"
     gene            complement(2814..3782)
                     /locus_tag="PKH10_07520"
     CDS             complement(2814..3782)
                     /locus_tag="PKH10_07520"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT28011.1"
                     /translation="MLNPDGWDPKNHAALTAFLDAEAKRPGRKVAVFDWDNTVIKNDI
                     GDAAFIGLLDRGMVATPDDWRRTNPYLTDAAVQALKKGATPELIFTIYNSMKTPDGSL
                     AFAGHDPKITEAAYAWLGHLLAGNTPDAIRRASREIFERQLANPIGATRRIGNDTIAD
                     YIRIYPQIADLIGGLERSGFEVWVVSATPKYIIDPCVAMVGIPQERVIGIENVVGPDG
                     KLTYDLIGCGPYGNDSRIISFRDGKRCWINETIFGMTSGDPLLPNPDPEKRPRFGAGD
                     SDGDISFLEDTTGLRLFIDKGRPLDVQKEARANKDGRWLIQEPFIG"
     gene            3940..>4480
                     /locus_tag="PKH10_07525"
     CDS             3940..>4480
                     /locus_tag="PKH10_07525"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNT28012.1"
                     /translation="MKQVTMMIVAAVLAVSFTLLGAEEPKKEEAVKEEVKKEEPKKEE
                     SLVPEWIKNIKLKGDFRFRYQYQNFEGKTEADSFERNRLRYRARIGLEAKVNDWAKVN
                     FGIASGSYESKITADQLAAGDDVKASLGQDLRSTNQTFDDFFSHKPVWIDYAFAEFTP
                     WSWLTLTGGKMKNPFHTTSE"
BASE COUNT          857 a         1312 c         1383 g          928 t
ORIGIN      
        1 gaccactcga cgccgtcggc ggagttccac acccccgccg tatcgaccgt ccagaggaga
       61 tcgtccttca ccacgaccga gcggttgccg tcgaaagagg gggccacctc ggtcgccatg
      121 agcgtccatg acactccgtc gtccgaacgc cacgcgtcgc gtttcttctc gccgccgaaa
      181 agccacaatc ttccctcgaa cggggtgacg gcgggatagt tgcgaccgtc gaacatcccc
      241 tcctccacct cttcgcttgc ggcatgcatc agggtccaca cgaagatgcg atgaccctcc
      301 ggcgcatcgg cgtccgacag gagatcgtcg ccggtctcat cggaaaggag cgcggtgtcg
      361 gtaaggtcat cggaccgttc atcgtcaggg agcggttcct cctcggcgac ggggtcatct
      421 tcgtcggaaa gaagcgttcc ggtgtcggta aggtctgtgt cggaaaaagt cgtcgcatcg
      481 gcatcggcgg cgggataaga ggccttcccg caaccgagga ggaacataag cggtatcagc
      541 aggatcgccg cccggaccgc tatcgcgaaa aaagagcgtt ccgcgtacat cccgtctccc
      601 ctctgttccg ttcgatgcct ctttgtacca tacccttcgg taaaagtcca gaccgttctc
      661 cgcaccgaaa agttgccatt gttcgtccgg atgctatgct tgcggcggcg ttcagagagg
      721 gcaagcgaat gaaggaccgg ttcggggcga tcgtttcgtt cctcgggctc aagcggagca
      781 tcgtcggcct gctgacgatg gtggtgctcg tgggactcgg cgagaagatg gccgaacggt
      841 tcctgccgct ctacctcatc gcgctcggcg cttcgccgct catcccggga ctcctcaacg
      901 ggctcgacaa tctcctctcg gcgctctatt cctatcccgg cggctggctg accgaaaaac
      961 tgggctacaa acgggcgctt gcggtcttca atctcgttgc gatgggcggc tttctcatcg
     1021 tcatcgcctt cccgcactgg atcgcggtca tcgtcgggtc ggtcttcttc ctgtcgtgga
     1081 cggcgctgtc gctccccgcg acgatggaac tggtgtcggc ggtgttgccc aagaacaagc
     1141 ggacgatggg ggtatcggtc cattcgctgg tccgccgcat cccgatggcg ctcgggccgg
     1201 tcatcggcgg ggtgctcatc gacctgtatg gcgtgaccac cggcgtccgc ctttcgttca
     1261 ccgtcgcgtt catcctcggc gcggtgtcgc tcgtcgtcca gcaagggatg atcgaggaac
     1321 ggcgcgagga aaaagaaccg gcgacgggcg gtctgcggtc gttcatcgca ctgatgccga
     1381 aggagttgaa agcgctcctc gcctccgata tcctgatacg tttctgcgaa cagatgccct
     1441 acgcatacct cgccatctgg gcgatgcaga gtccgcaggg tgcccgcgtg agcggcaccg
     1501 aattcggcat cctcacggcg atagagatgg tcgtcgcgct tctcatctat gtgccggtcg
     1561 cctatctcgc cgacaagggg gagaagaagc cgttcgttct cataactttc tttaatttca
     1621 cgatattccc ggtcattctc ctcttctcga ccggctttac gatgctggtg gtcgcgttcg
     1681 tcgtgcgcgg cctcaaggag ttcggcgagc cgacccgcaa ggcgctcatc atggacctcg
     1741 cccccgaggg acgcaaggcg gcgaccttcg gttcttatta tctcgtccgc gacaccgtcg
     1801 tatcgctcgc cgcctttgcc ggtgcgtggt tgtggcaggt gtcgccggcg gtcaatctcc
     1861 tgacggcggc cgcctgcggc gcgctgggaa ccatcctttt tgcggtgacg gggaaggaga
     1921 gggtctgatc agcgaaaact tgccttttat atcgggaaag cgccgactat actggtcact
     1981 taccttggga gggtattatg aaaagttctg ttgtttttat gctgtgcctg ttatcgatgc
     2041 tgttcatcgc ttgcgggggt gaggagaacg atccggtcga tagcgaaccg gtcaacgacg
     2101 atgcgacaac acccgacaac gataccgccg gcaccggcac gctggtcttc gccggcgtgg
     2161 ccaacggtac ggcgcccatg accgggattg tcggactgaa agaggtcatc gccgcgtcgc
     2221 agaccaacct catgcacggc accggactgg agctgtgggt caccgagata tcggtctcgc
     2281 agggactcgt cgccgacggg gtcgccgaca ccttcgtctg ggagaagatc ggagcgggcg
     2341 acgcgatcaa gccgataccg gaatactcgt tcacccatga ttccctgccg ataggcgaat
     2401 accagagcat caagatggtc ttcaagaact ccatcgtgat gacggcggtc taccacgacg
     2461 accagaccac ggagatcgag atgcccagtt ctctcgatca gggttcctgc ggcgacgaga
     2521 cgctcatcac gcagtatttc tcgccgaacg gcaatcacgc gctcgaaggc ggggtattcc
     2581 atgtgcagtc ggcgggcgag aaatcgagcg ccttcaccat cagcgaggga aagaccacga
     2641 cgatcaaatg gcagctgggc aagccgggcg tacagctgac cgactgttcc ttcgaatggg
     2701 tcgacgcgaa cggcaacggt tcgtgggatt gcggggtcga cagcgcgcag aacgcccagt
     2761 gcagcgtccc gaacgtcatg ttctacttca cggtcgaaga ggagtgatct ccgttaccct
     2821 atgaacggct cctgaataag ccatctgcca tccttgtttg cgcgcgcctc tttctgcaca
     2881 tccagcggcc gtcctttgtc gatgaaaagg cgcagaccgg tcgtatcctc aaggaaactg
     2941 atgtcgccgt cggaatcgcc cgcgccgaag cggggccgtt tttccgggtc aggattcggc
     3001 aggagcggat cgccggaggt catgccgaag atcgtttcgt ttatccagca tcgcttcccg
     3061 tcgcggaaac tgattatgcg gctgtcgttc ccgtagggtc cgcagccgat gaggtcgtag
     3121 gtgagtttcc cgtcggggcc gacgacattc tcgatgccga tgacccgttc ctgcgggatg
     3181 ccgaccattg cgacgcaggg gtcgattatg tatttcggcg tcgccgagac gacccacacc
     3241 tcgaaaccgc ttcgttccag tccgccgatg agatcggcga tctgcggata gatgcggata
     3301 tagtcggcga tggtgtcgtt cccgatgcgg cgggtcgcgc cgatcgggtt tgccaattgt
     3361 cgttcgaata tctcgcggga tgcccgccgt atcgcgtcgg gagtgttccc ggcaaggaga
     3421 tggccaagcc acgcgtaggc cgcctcggtt attttcggat cgtgtcctgc aaaagccagt
     3481 gacccgtcgg gggtcttcat cgagttgtag atcgtgaaga tgagttcggg agttgcccct
     3541 tttttgagcg cctgcactgc cgcgtcggtg agataggggt ttgtccggcg ccagtcgtcg
     3601 ggggtcgcga ccatgccgcg atcgagcagg ccgatgaagg cggcgtcgcc gatatcgttc
     3661 ttgatgacgg tgttgtccca gtcgaacacc gctaccttac ggccgggtcg tttcgcttcg
     3721 gcatcgagaa acgcggtcag cgccgcgtga tttttcgggt cccatccgtc ggggttgagc
     3781 atatcattct ccgtatccgc gggtcgaatc tagcagaatc gcgatcggaa cacaagaacg
     3841 gatgatttga cagagcattc accacgggtt aatactccgc taacattcgc cgggtatcca
     3901 tcaggccgtg catggtggat atttaatatg gaggaccgaa tgaaacaggt gacaatgatg
     3961 atcgtggccg ccgtgttggc ggtgagtttt acgctcttgg gggccgagga gccgaagaaa
     4021 gaagaagcgg tcaaggaaga ggtaaagaag gaagagccga agaaggagga atcgttggtg
     4081 cccgagtgga tcaagaacat taaactcaag ggcgacttcc ggttccgtta tcagtaccag
     4141 aatttcgaag gcaaaacaga agcggattcg tttgagcgca accggctccg gtaccgggcg
     4201 cgcatcggtc tggaggcgaa ggtcaacgac tgggcgaagg tcaacttcgg tatcgcgtcg
     4261 ggttcctacg agagcaagat aacggccgac cagttggccg ccggtgacga tgtcaaggcg
     4321 tcgctcgggc aggatctccg gtcgaccaac cagaccttcg acgatttctt cagccacaaa
     4381 ccggtctgga tagattacgc cttcgccgaa ttcacgccgt ggagttggct cacgctcacg
     4441 ggcggcaaga tgaagaaccc gttccacacc acctccgaac
//
LOCUS       DAOQUE010000217         4469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_21732_length_4469_cov_3.743543, whole genome shotgun sequence.
ACCESSION   DAOQUE010000217 DAOQUE010000000
VERSION     DAOQUE010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21732_length_4469_cov_3.743543"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PKH10_07530"
     CDS             complement(<1..318)
                     /locus_tag="PKH10_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28013.1"
                     /translation="MRVFLVILMAFFLMFMVSCPSKKAPPITVNIPADSAKLKAHVEY
                     LAGLHPTRSSNNEDSHEAAAKYIEEQFAAVGCAVTMQRYTIDAGEFRNIICRYGQADA
                     PRII"
     gene            complement(322..855)
                     /locus_tag="PKH10_07535"
     CDS             complement(322..855)
                     /locus_tag="PKH10_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28014.1"
                     /translation="MNPKEKIKELALSFSNISSEDENDTGPVGYLIGAMYCLQKAIDL
                     GHKDGLLIEDDDNYRLELQEVAKKVAENNDPREVWGTRKRNDKMQGVWLAGCYFNMAM
                     QKIDAVRERIGIEAKKNRDLELVYNDVNCLKHHDPQIDPTRKKKGLLPGRTATIDNVI
                     GAIKFLREHFQRKGVLG"
     gene            complement(859..1671)
                     /locus_tag="PKH10_07540"
     CDS             complement(859..1671)
                     /locus_tag="PKH10_07540"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNT28015.1"
                     /translation="MNMIRYAVVLAVLCPVLLCTCSKKDSFAPPLDRAMIAEKEESAG
                     ARKSIGGQTVASDVQERRLIKTGTVTFRTDSVSATGRQVRDMLKTHNGYVAQEDEYGH
                     GGSKSFTIIARIPAASFDRFVEELEKALGPFDARSIGVQDVTAEFVDTEARLKAKKEV
                     EKNYLDILSRAKTIEETLAVQKLLGEVRGEIESVEGRLKLLQDQTAYSSLTISFYERS
                     VTGFSFLGDVKDAFKAGWTGFLQVLVGIAYAWVFIIIGVGIVVYRKVRRKKG"
     gene            complement(1734..2885)
                     /locus_tag="PKH10_07545"
     CDS             complement(1734..2885)
                     /locus_tag="PKH10_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28016.1"
                     /translation="MLYIVIDGALFMVKEGRPVPVPNGFIAEREKEAERQKGLTDWRN
                     AQREQQDPYFNAATIWGASRGRYTGRWRFHSAAVTRDGSICYTMKNALVTALFRYDPA
                     VNEDRRLFHKSDFHEEGMTALPDGRIVVALRDEKGVVDLALFDGEMKFDGYLTGGDSI
                     DQLPTASLADGDRVLYSSRGIARTEQGVVAALGPSSLFSVAVARREVTEVATEPEKDL
                     LLPKEGADGSLFYIRSSYEAPFRPGLLQTFKDALLFPVHLLFAIFGFLTVFVKLFDRR
                     PPKPLGPQAVPPQESAWRTILGKAVDTRTVEKNAGKGEGPALVPRSWELVRRSPDGAE
                     EVIARNVAAFDLASDGTVYFTNGYKLYSFDGKDIKTVLKEDLMERVFCA"
     gene            complement(2900..4075)
                     /locus_tag="PKH10_07550"
     CDS             complement(2900..4075)
                     /locus_tag="PKH10_07550"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT28017.1"
                     /translation="MRYLLLLLLLLAVASTMGDSGQRVTKLAVMEIEDLSGTLDKKIL
                     ENAAEALRGELVASNKFVVISKDRQQKQAVKEMKKESWKECYDQSCRIQLGQALTADT
                     ILTGKITLFGKSYTLMVELIDLAKEATIKGAKVDFDGTENGLADAVRNVAVEITGVGT
                     IRKKVDTEGDARACAMAKEKDTHAMWSVYIEEFPKGACAFEAKSRIKELAATVKAPAA
                     QPAEPEKKAEAKPSNMVANRYKLYPEMVLDTKHKLLWQRRRSNMPMTHDNAVKYCQDL
                     YIGDYGHWRLPTIGELKTIIHGCQSASDACPVHDGCLSGKCYNAKCYCWHGGGPGEDG
                     YFWQKDVWHGGTTFWSSSILQGSSSATHAWFADFATGKISNIGRYNKYDVRCVKKVK"
     gene            complement(4179..>4469)
                     /locus_tag="PKH10_07555"
     CDS             complement(4179..>4469)
                     /locus_tag="PKH10_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008859876.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNT28018.1"
                     /translation="IYTGLRQTPESIANAAVQEDVDAVGLSILSGAHNTLLPKVVELL
                     KAKGAGDILVFGGGVIPEDDIPGLKAQGIAAVFTPGTPSDQFIKFIKEHIRK"
BASE COUNT          864 a         1374 c         1188 g         1043 t
ORIGIN      
        1 aatgatgcgc ggtgcgtcgg cctgcccata ccggcagatg atgtttcgga attcaccggc
       61 gtcaatcgta taacgttgca ttgtaacagc gcagcccacc gccgcgaact gttcttcgat
      121 gtactttgcc gccgcctcgt gcgagtcttc gttattggaa ctccgcgtcg gatgaagtcc
      181 cgcgaggtac tcgacatggg ctttgagctt cgcgctgtcg gccggaatgt tgaccgtgat
      241 cggtggcgct ttcttgctgg ggcaggaaac cataaacatt aaaaagaaag ccatcaagat
      301 gacgaggaaa actctcatga cttatccaag gactcctttt cgttgaaagt gttctctcaa
      361 aaactttatg gcacctatca cattgtcaat tgttgcggtc ctccccggca aaagaccttt
      421 ctttttcctt gttgggtcta tttgagggtc atgatgtttt aggcaattca catcgttata
      481 aaccagttcc aaatccctat tcttttttgc ttcaatccct attctttctc tcacggcgtc
      541 aatcttctgc atggccatat taaaataaca accggcgagc caaacacctt gcattttatc
      601 atttcttttt cttgtgcccc agacctctct tggatcgttg ttctctgcta cttttttggc
      661 aacttcttgt aattctagtc ggtagttgtc atcgtcttct atcagtagac catctttgtg
      721 tcccaaatcg atggcctttt gtagacaata cattgcacca ataagatatc caacaggacc
      781 agtatcattt tcatcttcac tagagatgtt cgaaaatgaa agagccaatt cttttatttt
      841 ctcttttgga ttcatgggct accctttctt ccgccgcact ttacgataga cgacgatgcc
      901 gacaccgatg atgatgaata cccacgcgta ggcgatgccg acgagcacct gcaggaaccc
      961 ggtccacccc gccttgaagg catccttcac atcaccgagg aaggagaacc ccgtcacact
     1021 gcgctcgtag aacgatatcg tgagcgaact gtaggccgtc tggtcctgca acagtttcag
     1081 ccgcccctcg accgattcta tctcgccgcg cacctcgccg agcagtttct ggaccgcgag
     1141 ggtctcctct atcgtcttgg cccgcgacag gatatcgaga tagttcttct cgacctcctt
     1201 cttcgccttc aggcgcgcct cggtgtcgac gaactcggcc gtcacatcct gcacgccgat
     1261 ggaacgcgca tcgaacggcc ccagcgcctt ttcgagttct tcaacgaagc ggtcgaacga
     1321 tgcagccggg atgcgggcga tgatggtgaa actcttggaa ccaccgtgcc cgtactcatc
     1381 ctcctgcgcc acatagccgt tgtgggtctt gagcatatcg cggacctgcc gtccggtggc
     1441 cgacaccgaa tcggtgcgga aggtgaccgt cccggtcttg atcaggcgtc gctcctgcac
     1501 atcggacgcc acggtctgac cgccgatgct tttcctcgca ccggccgatt cttccttctc
     1561 ggcgatcatc gcccggtcga gcggcggcgc aaaagaatcc ttcttcgagc aggtacaaag
     1621 cagtaccgga caaagcaccg caagaacgac ggcataacga atcatgttca tggtatcacc
     1681 cccgtgtatt tccgatatcg gcctgcccat tttcttcgat tcgctactga aagtcaagcg
     1741 cagaaaacgc gctccatcag gtcttcttta aggaccgtct ttatatcttt gccgtcgaac
     1801 gaatagagtt tgtatccgtt ggtgaaatag accgtcccgt cggaggcgag atcgaaggcg
     1861 gccacattcc gagcgatcac ctcctccgcg ccatcgggcg aacgacggac cagttcccat
     1921 gagcggggca ccagcgccgg tccttccccc ttcccggcgt tcttttcgac cgtgcgggta
     1981 tcgaccgcct tgccgaggat ggtgcgccat gcgctctcct gtggcggcac cgcctgcggg
     2041 ccgagcggtt tcggcgggcg ccggtcgaac agtttcacga acaccgtgag aaaaccgaaa
     2101 atggcgaaca ggagatgcac cgggaacagt agagcgtcct tgaacgtctg aaggagaccg
     2161 ggacggaagg gcgcttcgta ggacgaccgg atgtagaaaa gcgagccatc ggccccctct
     2221 ttcggcagaa gcaggtcttt ctccggttcg gtggccacct cggttacttc ccgccgtgcg
     2281 accgcgaccg aaaaaaggga gctcggccca agcgccgcaa ccacgccctg ctcggtgcgg
     2341 gcgatcccgc gcgacgaata caggactcga tcaccgtccg cgagagacgc cgtggggagt
     2401 tggtcgatcg aatcgccgcc ggtcaggtag ccgtcgaact tcatctcccc gtcgaacagc
     2461 gccagatcga cgacaccctt ctcgtcgcgc agggccacca cgatacgacc gtcgggaagc
     2521 gccgtcatcc cctcttcgtg aaaatcgctt ttgtggaaca ggcgccggtc ctcattgacg
     2581 gcagggtcat atctgaacag cgcggtcacg agcgcgttct tcatcgtgta acatatcgag
     2641 ccgtcacgcg tcacggcggc ggaatgaaaa cgccaccggc cggtatagcg tccccgcgac
     2701 gcaccccata tcgtcgccgc gttgaaataa gggtcctgtt gttcgcgctg ggcattgcgc
     2761 caatcggtga ggcctttctg ccgctcggcc tctttctccc gttcggcgat gaacccgttc
     2821 gggaccggga ccgggcggcc ctccttcacc atgaaaagcg cgccgtcgat aacgatgtag
     2881 agcatgccgg acacgggaac tacttcacct tcttcacgca acggacatcg tacttgttgt
     2941 agcgtccgat attgcttatc ttgccggtcg cgaagtcggc gaaccacgca tgggtggcgc
     3001 tgctgctccc ctgcaggata gaggatgacc agaaggtggt cccgccgtgc cacacatcct
     3061 tctgccagaa atagccgtcc tcgccggggc cgcccccgtg ccagcaataa catttcgcgt
     3121 tgtagcactt accgctcaga cacccgtcat gcaccggaca ggcatcgctg gcgctctggc
     3181 acccgtgaat gatcgtcttc agttcgccga tggtcgggag ccgccagtgg ccgtaatcgc
     3241 cgatgtagag gtcctgacag tatttgaccg cgttgtcgtg ggtcatgggc atattcgacc
     3301 gccttctctg ccacagcagt ttgtgtttcg tgtcgagcac catctccggg tacagtttat
     3361 agcggttggc gaccatgttc gacggcttcg cctcagcctt tttctccggc tcggcgggtt
     3421 gcgccgccgg cgccttgacg gtcgccgcca actccttgat gcgcgatttt gcctcgaagg
     3481 cgcaggcccc tttcgggaac tcttcgatgt agacgctcca catcgcgtgg gtatccttct
     3541 ctttcgccat ggcgcaggcc cgagcgtcgc cctcggtgtc gacctttttc ctgatcgtgc
     3601 cgacgccggt tatctccacc gcgacattcc gcaccgcgtc ggcgagtccg ttctccgtgc
     3661 cgtcgaaatc gaccttcgcc ccctttatcg tcgcctcctt ggcgagatcg atgagttcca
     3721 ccatcagagt gtaggacttg ccgaacaggg ttatcttgcc ggtcaggatg gtgtcggccg
     3781 tcaacgcctg ccccaactgg atgcggcacg actggtcgta acactccttc cacgactctt
     3841 tcttcatctc cttgaccgcc tgtttctgct ggcggtcctt ggagatgacc acgaatttgt
     3901 tcgacgcgac cagttcgccg cgcaacgctt cggccgcgtt ctccaggatc tttttgtcga
     3961 gcgtgcccga cagatcctcg atctccatga ccgcgagttt cgtgacccgt tgccccgaat
     4021 cgcccatggt cgacgcgacc gcaagcagta acaggagaag aagaaggtac ctcatgcgca
     4081 ccccgcagtt ttggacttaa ttgatgaggt tatttttcat atttttcttt gaaagtcaaa
     4141 agaaaaacag ggaaattgtc aggcgggacg gatgccgttc acttcctgat atgttccttg
     4201 atgaacttga tgaactggtc gctcggggtg ccgggggtga agaccgccgc gatcccctgc
     4261 gccttgagcc ccggaatatc gtcctcgggg atgacgccgc cgccgaacac aaggatatcg
     4321 cccgcccctt tcgccttcag gagttcgacc accttcggca ggagcgtgtt gtgggcgccc
     4381 gacagtatcg agagtcccac cgcgtcgaca tcttcctgca ccgccgcgtt cgctatcgat
     4441 tcgggggtct gccgcaggcc ggtgtagat
//
LOCUS       DAOQUE010000218         4445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_21894_length_4445_cov_3.557403, whole genome shotgun sequence.
ACCESSION   DAOQUE010000218 DAOQUE010000000
VERSION     DAOQUE010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21894_length_4445_cov_3.557403"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKH10_07560"
     CDS             <1..420
                     /locus_tag="PKH10_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28019.1"
                     /translation="YPMGHGLGVYATSEEAAPSLMLKNVVVDRNQNGIQMQYAKVFAG
                     TDCTLPMGSKKETPVCVHVLNNSVGLNVFDPPAGYDRDAAFALQREGGTWYNGNDTPF
                     SSDKEEVSQPPDPQQAPAPSVLENMPSLFLFLTSLDS"
     gene            417..644
                     /locus_tag="PKH10_07565"
     CDS             417..644
                     /locus_tag="PKH10_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28020.1"
                     /translation="MNRSRLAIFFFFCGTVCLYIVGHETPINAFELPEDYDMYAAFAE
                     SDTWYEDNRGPISSEKQATGESVDASEMKNP"
     gene            653..3406
                     /locus_tag="PKH10_07570"
     CDS             653..3406
                     /locus_tag="PKH10_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT28021.1"
                     /translation="MRKDLFLFFLLSWVICSCAGKSAAPKDHDLAGPETDAILNDEDL
                     SVADELLPDDAPPVVSDPRPPDWGDCTGGWEQKEQINEQTGRLEARWCEPPADWRPAA
                     PPDWGDCPEGWEPKEEKDEAGDLLAKYCEPILPPEETVCPPGYFALFGSTECQRIGAE
                     CPAGEFADIPADAGTEIVYVSEGGSIQAAIDAAPEGAVIALGKGTFDEIVTIVNKGVT
                     LWGACVEETILTKKTHADAGGYALLNLEGNDATGTVVVRDLSVSDTASENLWGISVDG
                     GTAELKGVSIDRATIGIFVAWKAHLYAENVLIADMMSEEDGTKGMGLWLYQAEATLAR
                     TVIERTRWFGILADHFDSGRESVLTATDLIVRDTRSRVSDKTAGYGITLQGNTRATLS
                     RVLLERNREIGLFAIGTAIDATIEFSAADLIVRGTEPMEYLGTDGAGIHLQGYVTATL
                     DRTLVADNRTVGLSALAKPPTQVQLVTRDLIVSDTSSQQSDKNFGLGMDLQENVTADM
                     TRTALIRNRSMGLSVHTINNTRQAILQMTDAFVYGTLPQESDLTGGRGIQLENHVSAL
                     FRRVVITNNADIGIFVDTPAAGSEATVTIEDGVISDTVAQASDGMGGRGLNIQNETTA
                     DLARVVMERNRDVGLFIVTVNEGTHASLTAADLIVRDTRSRGNDGTAGRGMSLQNNVA
                     ATLGRVLVERNRELGMLVATSEQGVALTLDATDLVVRDTRSQDSDKQWGAGMLLQDGV
                     SGTIRRAFVDGNREAGIIITGDPAAGGAPQMDLIDIVISHTAIRECFELGIDCGYAPE
                     AAFGYGMVAQGTDGTTAVTLTDVAILDNETGFQIKGARVFAGDTCTLPFGLEEGKTAC
                     IHLVDNETALNAFSLPEDYDPNAAFAEFRTWYDGNELNFSGDPQAVPEPPDPTDPFED
                     GGK"
     gene            3463..>4445
                     /locus_tag="PKH10_07575"
     CDS             3463..>4445
                     /locus_tag="PKH10_07575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNT28022.1"
                     /translation="MKQDLLFSILAAQNGMIKPEDLIEIGSAWVANQKKGLKERLCER
                     LSREDIELLEKLVTRSLAKCGGDVDRSIQALHADDLLFKTLGGAIVVDVEPGKKEPCA
                     APVFCEKVTIESPDRYSMKVEVKRGGIGRIFVALDEHIGRNIAIKELLCEATDEGTVE
                     EKFSTQASKSLRARFLREARVTGQLEHPAIVPVYEIGQRLDGACYYTMRYVKGSTLLD
                     AIRKRTELSERLKLLPHFHDLCNAVAYAHSKGVVNRDIKSENVMIGEFGETVVLDWGL
                     AKVKGQEDIGAERIEEGIQLIKDSDVGKTTIGHAIGTPAYMPPEQWRDVALV"
BASE COUNT          935 a         1282 c         1428 g          800 t
ORIGIN      
        1 taccccatgg gacacgggct aggggtttac gcgacatcgg aagaggcggc gccgtcgctg
       61 atgctgaaga atgtagttgt cgacagaaac cagaacggta tacagatgca gtatgcgaag
      121 gtgttcgccg gaacggactg tacccttccc atgggcagta aaaaagagac gccggtctgc
      181 gttcatgtcc tcaataactc tgttggcctg aatgtcttcg atccccctgc gggctacgac
      241 agggatgcgg ccttcgccct gcaaagggaa ggcggcacat ggtacaacgg gaacgatacg
      301 cctttctcga gcgataaaga ggaggtctct caaccgccgg atccccagca ggcgcccgca
      361 ccctcggtgc tagaaaacat gccttctctt ttcctcttcc ttacttcctt ggattcatga
      421 accgttctcg gcttgcgata ttcttttttt tctgcggcac ggtctgtctc tacatcgtgg
      481 gccacgagac cccgataaac gcctttgaat tgcccgaaga ctacgacatg tatgccgcct
      541 tcgcggagag cgacacatgg tacgaggaca accggggccc gatatcgtcc gaaaagcaag
      601 ccaccggcga atcggtcgat gcatcggaaa tgaaaaaccc ctgaggttcg ggatgaggaa
      661 ggatcttttt cttttctttt tgctctcttg ggtcatttgt tcctgcgccg ggaaaagcgc
      721 ggcgccgaag gaccatgacc tcgcggggcc cgaaaccgac gcgatactga acgacgagga
      781 tctttccgtc gccgatgagc tgctccccga cgatgcgcct cccgtggtga gcgatccgcg
      841 tccgcccgat tggggcgact gcaccggcgg gtgggaacag aaagaacaaa tcaatgaaca
      901 gaccggtcgc cttgaggcgc gctggtgtga accgcccgcc gactggcgtc ccgccgcgcc
      961 gcccgactgg ggcgattgtc ccgaaggatg ggagccgaag gaagagaagg acgaagcggg
     1021 ggatctgctc gccaaatact gcgagccgat actgccgccc gaagagaccg tgtgcccgcc
     1081 gggctacttc gccctgttcg gttcgaccga atgccagcgg ataggggccg agtgtcccgc
     1141 gggcgaattt gccgacattc ccgccgacgc cgggaccgag atagtgtatg tctccgaggg
     1201 aggatccatt caggcggcga tagacgcggc gccggagggc gcggtcatcg cccttggcaa
     1261 ggggaccttc gacgagatcg tcaccatcgt caacaagggg gtgacgctgt ggggcgcctg
     1321 cgtcgaagag accatcctga ccaaaaagac ccatgcggat gccggggggt atgcgttgct
     1381 gaacctagag gggaacgacg ccaccggaac ggtcgtcgtg cgcgaccttt ccgtttccga
     1441 caccgcttcc gagaacctgt gggggatcag cgttgacggt ggcacggccg aactgaaagg
     1501 ggtctcgata gaccgggcca cgattggcat cttcgttgcg tggaaggccc acctgtacgc
     1561 cgagaatgtg cttattgccg acatgatgtc tgaagaggac ggtacgaagg ggatggggct
     1621 ctggctctat caggccgaag cgacgctagc ccgcaccgtt attgagcgaa cgcgctggtt
     1681 cgggatactt gccgaccact tcgattcggg gagggagagc gtattgaccg cgaccgacct
     1741 catcgtccgc gatacccggt cgcgggtgtc cgacaagacc gccggctacg gcatcacgct
     1801 tcaaggcaac acccgcgcca cgctgagccg cgttcttctg gagcgcaacc gggagatcgg
     1861 gctgttcgct atcggcactg cgattgacgc cactatcgaa ttctcggccg ccgacctcat
     1921 cgtgcgggga acagagccta tggaatacct cggcaccgac ggcgccggga tacatctgca
     1981 ggggtatgtc accgccacgc tcgaccggac gctcgtcgcg gacaaccgca ccgtcggcct
     2041 gtccgcgctc gccaagccgc cgacgcaggt acagctggtc acgagggatc tcatcgtctc
     2101 ggataccagt tcccagcaga gcgataagaa cttcggactg gggatggatc ttcaggagaa
     2161 tgtgaccgcg gacatgacgc ggacggcgtt gatacggaac agaagcatgg gcttgtcggt
     2221 ccatacgatc aacaacaccc ggcaggcgat cctgcagatg accgatgcct ttgtgtacgg
     2281 cacccttccg caggaaagcg acctgaccgg cggccggggc attcaactgg agaaccatgt
     2341 ttccgcgctg ttccgccggg tcgtcatcac gaacaacgcc gatatcggca tctttgtcga
     2401 tacgcctgcc gcgggatcgg aagcgacggt caccatcgag gatggggtca tcagcgatac
     2461 ggtggcgcag gcaagcgacg gtatgggggg aagagggctc aacatacaga acgaaacgac
     2521 cgccgacctc gcgcgggtag tgatggagcg caaccgtgat gtggggctct ttatcgtcac
     2581 ggtcaacgag gggacgcatg cttcgttgac ggccgccgac ctcatcgtgc gcgatacccg
     2641 atcgcgcgga aacgacggca ccgccggcag agggatgtcc ctgcagaaca atgtcgcggc
     2701 gacgctcggg cgggtccttg tggagcggaa ccgggaactg ggcatgctgg tcgccacaag
     2761 cgaacagggc gttgcgctca ccttggacgc gaccgatctc gtcgtccgcg atacgcggtc
     2821 acaggatagc gacaagcagt ggggcgcggg gatgctcctg caggacggcg tcagcggtac
     2881 catccgcaga gcgttcgtgg acggcaaccg cgaagcgggc atcatcatca cgggcgatcc
     2941 tgcggcgggt ggcgcgccgc agatggacct gatagatatc gtcatctcgc ataccgcgat
     3001 ccgcgagtgt tttgaacttg ggatcgattg cgggtacgcg ccggaggccg cctttggata
     3061 cggcatggtg gcgcagggga ccgacgggac caccgcggta acgctcaccg atgtggctat
     3121 cctcgataac gagaccggct ttcagataaa gggtgcgcgg gtctttgccg gcgatacctg
     3181 tacccttccc tttggtcttg aagaggggaa gaccgcctgt atccacctcg tcgacaatga
     3241 gaccgcgctc aacgcgttca gtctgcccga agactatgac cccaatgccg cctttgccga
     3301 gttccggaca tggtatgacg gcaacgaact taatttttcc ggtgatccgc aggcggtgcc
     3361 ggagccgccc gaccccacgg acccctttga ggacggcggg aaatagttga ttgtcgatgg
     3421 gtgttggggt atcctgaagg cccttaaggg atagagggcg tcatgaaaca agacctgctg
     3481 ttctcaattc tcgcggctca gaacggtatg ataaagccgg aggaccttat cgagataggg
     3541 tccgcgtggg tggcgaacca gaaaaaaggg ctcaaggaac ggctctgcga gcggctgagc
     3601 cgcgaagaca tcgaactgct ggaaaaactg gtgacccgct cgctggcaaa gtgcggcggc
     3661 gatgtcgacc ggtccataca ggcgttgcat gccgacgatc tcctcttcaa gacgctcggc
     3721 ggcgcgatcg tcgtggatgt cgaaccgggg aagaaagagc cctgcgcggc tccggtgttc
     3781 tgtgaaaaag tgaccatcga gagccccgac cgctattcga tgaaggtcga ggtcaaacgc
     3841 ggcggtatcg ggcggatatt cgtcgcgctg gacgaacaca tcggccggaa tatcgcgata
     3901 aaggagttgc tctgcgaagc gaccgacgag ggcacggtgg aggaaaagtt cagtacgcag
     3961 gcttcgaagt cgttgcgcgc ccggttcctc cgcgaagcgc gggtgaccgg ccaactggag
     4021 catcccgcca tcgtgccggt gtacgagatc ggtcagcgtc tcgatggcgc ctgctactac
     4081 accatgcgct atgtcaaggg gagtaccctt ctggatgcga tacggaaaag gaccgaactt
     4141 tcggaacggc tgaaactcct gccgcatttc cacgatctct gcaacgccgt cgcctatgcc
     4201 cacagcaagg gggtcgtcaa ccgcgacatc aagtcggaga atgtgatgat cggcgagttc
     4261 ggcgagacgg tggtgctcga ctgggggctt gccaaagtga agggacagga ggatatcggc
     4321 gccgagagga tcgaggaagg catccaactt atcaaggact ccgatgtggg aaagaccacc
     4381 ataggacacg ccatcggcac tcccgcctac atgccgcccg aacagtggcg cgatgtcgcg
     4441 ctggt
//
LOCUS       DAOQUE010000219         4443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_21903_length_4443_cov_3.926846, whole genome shotgun sequence.
ACCESSION   DAOQUE010000219 DAOQUE010000000
VERSION     DAOQUE010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21903_length_4443_cov_3.926846"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1924
                     /locus_tag="PKH10_07580"
     CDS             <1..1924
                     /locus_tag="PKH10_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28023.1"
                     /translation="DGVSWAKVGDWQPVVSAPPGGYCEPLGDGLYGPVAAPVGDRLIA
                     LGGHFHDSCYFQASLPGYSVGWSQYYYADTYTTYATFRAGSFSSHAIPDIDINNTWYR
                     VFGSYGAAVPFDGDLWVFAGQWQNSDTVQQDIGPGAGPPQVWPGGLTPLPGIAVTADG
                     IHWEPAPRRLPLSQMYGFGAASFNDAVWLIGGNGGNAIWRGSYVSLPGGDPEAECILG
                     AYPGGSLAVDSNEGAELLRGYTSAKTLYVQGSKVSDLSALHCLKEVEDLYWGSQMGPG
                     DDPAVTAFLGLTGLETITSRLYAYYTSLSSLSDFPALRLIGKEAIVYYGTAETFTIDM
                     LTVAVPELTIAGSDTLATIDLQRANAGSTIKVNGNTALRTILWPLLEEVRSVSFYNNP
                     LLETITLPSLRSIESLGLQDNPVLVSIEGFPDVVSLPMLTINDCPAIENFELTSRLTT
                     VPYLYVKGDYDIGMPPLFPALTSVDDTLLLSGIFPVEMGTFLAPQATVAALRIDHLSG
                     PPDLSFLAGARVGAFYCYYCKMPDLTGIVAQEEMSYLYIKDSDFFTSLAPLADLRRVT
                     NELYLFSNTALADIAPLMAVEEVPATLSFLIKVNPALSSADVQRVVDHFVQDLGLPPE
                     QVDVGYNCDACPPLIP"
     gene            2001..2900
                     /locus_tag="PKH10_07585"
     CDS             2001..2900
                     /locus_tag="PKH10_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28024.1"
                     /translation="MERLALLLFIAALGLCGACENSTMKLVDADTAQNDTAAVTDDDT
                     AQSDEVNNEANDGTGDEDLTPSDGILPDEDDLLPDGDGQPCVGYAADCGAGFYCLTEI
                     GDCQGIGVCTPVWTEECPLLLDPVCGCDGSTYTSDCDAAASGMALSHNGKCPTPATST
                     LTYNNTVEPWPGGANILEGSASFVSSPVDPAEVFTVPAPFETNKSFVTFVIYKQNNSP
                     EHLLVTLSRVEMGGTLPVTVTLQGPPDNAGLSRAERYYDTGIFGHLTGDVTITAFDEG
                     GMPGAPVTLVLSGDALTFSWVDQ"
     gene            complement(2944..3639)
                     /locus_tag="PKH10_07590"
     CDS             complement(2944..3639)
                     /locus_tag="PKH10_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28025.1"
                     /translation="MSIVIDDIKKMAALVGSLSRPAVFVGGAVAVLYTDRPEFSEMRA
                     TKDIDLVFKIASLTQLETVREELVRAGFSHVIGSPVVCRFAFERILVDVMALDAVGWA
                     PGNHWFKRGFNRATPYSLDGAALSLLPFPYYLASKFDAFLGRGMRDAYASHDLEDIVF
                     VLDGRKNFAQELIGYDDREVISFLRTNMEKLMTDERLKEAVLGHLPFRFQEERFKRLQ
                     QSIALFCDVGNGG"
     gene            complement(3636..4145)
                     /locus_tag="PKH10_07595"
     CDS             complement(3636..4145)
                     /locus_tag="PKH10_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28026.1"
                     /translation="MKQHNGMRPLDIVVLLKITGMKEPWFLKDVADSLRISRSEVSES
                     VQRSAMAGLLAADKKTVMRAALKEFLIHGIRYVFPQVPGTLTRGVPTAHSAKPLSDEI
                     GGGDAFVWPHIDGAVRGQAITPLYPTVPDACLADERLYELLALVDALRVGRARERELA
                     VKELEARLT"
BASE COUNT          877 a         1400 c         1273 g          893 t
ORIGIN      
        1 cgacggcgtt tcatgggcaa aggtcggcga ttggcagccg gtcgtctcgg ctcctccggg
       61 cgggtactgc gagccgctcg gcgacgggct gtacgggccg gtcgcggcgc cggtcggcga
      121 ccggttgatc gcgctcggcg gccacttcca cgattcgtgc tatttccaag cgtcgctccc
      181 cggctattcg gtcggctggt cccaatacta ctacgccgac acctacacga cctatgcgac
      241 cttccgcgcc ggatcctttt cttcgcacgc gatacccgat atcgacatca acaatacctg
      301 gtatcgcgtg ttcgggtcgt atggcgccgc cgtgccgttc gacggggacc tgtgggtatt
      361 cgccggacag tggcagaaca gcgacacggt ccagcaggat atcggtcccg gcgcgggacc
      421 gccgcaggtc tggccgggcg gcctgacgcc gcttcccggc atcgcggtca ccgccgacgg
      481 catccactgg gaaccggcgc cgcgcagact gccgctgtcg cagatgtacg ggttcggcgc
      541 ggcgagtttc aacgacgccg tctggctgat cggcggcaac ggcggcaacg cgatatggcg
      601 cgggtcgtat gtctctctgc cggggggcga tccggaggcc gaatgcatcc tcggcgccta
      661 tccgggaggg tcccttgcgg tagattcgaa cgagggtgcg gaacttttga gaggatacac
      721 ctccgcgaaa accctctatg tgcaaggttc caaggtctcc gatctttcgg cgctccattg
      781 tctgaaagag gtcgaagatc tgtactgggg atcgcagatg ggccccggcg acgacccggc
      841 cgtgacggca ttcttgggct tgaccggact cgaaacgata acttcccgcc tgtatgcgta
      901 ttatacctct ctttcctcgc tcagtgattt tcccgctctc cgcctcatcg gtaaggaggc
      961 gatcgtttac tatggcaccg cggaaacctt cacgatcgat atgctgaccg tcgcggtgcc
     1021 tgaactcacc atcgcaggga gcgacacgct ggccaccatc gacctgcaaa gagcgaatgc
     1081 cggttctacc atcaaagtga acgggaacac ggcgttacga acgatactat ggccgcttct
     1141 cgaagaggta aggtccgttt cgttttataa caaccctctt ttggagacga taacgctccc
     1201 ctctttgcgg tctatcgaat ctctgggact gcaggataac cccgtgctgg tatcgataga
     1261 gggctttccc gatgttgttt ctctccccat gctgaccatc aacgactgcc cggctatcga
     1321 gaattttgaa cttacctctc gcttgacgac cgtgccctac ctctatgtga aaggcgatta
     1381 tgacatcggt atgcccccgt tgttccccgc gcttacctcg gtcgatgata ccctgttgct
     1441 ttcggggata ttccccgtcg aaatgggaac ttttctcgcc ccgcaggcga ccgtcgcggc
     1501 gctcaggata gaccatctct ccggcccgcc cgatctttcg ttccttgcgg gggcccgcgt
     1561 cggcgctttt tactgctact actgcaagat gcccgacctg acgggtatcg tcgcgcagga
     1621 ggagatgagt tacctctaca tcaaagacag cgactttttc accagtctcg ccccgctcgc
     1681 cgacctgcgc cgggtgacca atgaacttta tcttttcagc aacaccgcgc tggccgatat
     1741 cgcgccgctc atggcggtcg aagaggtccc ggcgacactc tctttcctca ttaaggtcaa
     1801 ccccgccctc tcttcggccg atgtccagcg ggtcgtcgac catttcgtac aggacctcgg
     1861 gcttccgccg gaacaggtcg atgtcgggta caactgcgat gcctgcccgc cgctgatccc
     1921 ctgaagtcat ctctttactt tccccgtccg ttcggctatc atcggaaggt cttccccggt
     1981 ttttttaagg agatatcgtc atggaacgcc tcgccctgct ccttttcatc gccgcgctcg
     2041 gcctctgcgg tgcctgcgag aacagcacga tgaaactggt cgatgccgac accgcgcaga
     2101 acgacaccgc ggcggtgacc gacgacgata cggcgcaaag cgacgaggtg aataacgaag
     2161 cgaacgacgg gacaggggac gaagacctga ccccctccga cgggatactg ccggatgagg
     2221 acgacctgtt gcccgacggc gacggacagc cctgcgtcgg ctacgcggcc gactgcggcg
     2281 cgggcttcta ctgcctcacc gagatcggcg actgtcaggg gatcggcgtc tgcacgccgg
     2341 tctggaccga ggagtgtccc ttgctcttgg atccggtctg cggctgtgac ggcagtacct
     2401 acacgagcga ctgcgatgcc gccgccagcg gcatggcgct ttcccacaac gggaaatgtc
     2461 ccaccccggc gacctcgaca ctgacctaca acaacaccgt cgagccgtgg ccgggcgggg
     2521 cgaacatcct cgaagggagc gcctcgttcg tctcttcgcc ggtcgatccg gcggaggtct
     2581 tcacggtccc cgcgccgttc gagacgaaca agagtttcgt caccttcgtc atctacaagc
     2641 agaacaattc gcccgaacat ctgctcgtca ccctgtcgcg tgtcgagatg ggcggtacgc
     2701 tcccggtgac cgtcactctg cagggtccgc ctgacaacgc gggtctttca cgggcggagc
     2761 ggtactatga tacggggatc ttcggccatc tgacgggcga tgtcaccatc accgcgttcg
     2821 atgaaggggg tatgcccggg gcgcccgtca cgctcgtgct gtcgggcgac gcgctgacct
     2881 tttcgtgggt ggatcagtag aattccgcca gccaaccggc ggagaagcaa gagaaattag
     2941 gcctcatcca ccgttgccca catcgcaaaa aagagcgata ctttgttgca gccttttgaa
     3001 gcgttcctcc tgaaagcgga acggcagatg ccccagaacg gcctccttca gccgctcatc
     3061 cgtcatcaat ttttccatat tcgtgcgaag gaaagatatc acctcgcggt cgtcataccc
     3121 gatcaactcc tgcgcgaagt tcttgcggcc gtcgagcacg aaaacgatat cctccagatc
     3181 atggctcgca taggcatcgc gcatcccacg cccaaggaaa gcatcgaact ttgacgcgag
     3241 gtaatacggg aacggtaaca gcgacagcgc ggcgccatca agcgaataag gcgtggcacg
     3301 attgaatccc cgcttaaacc agtggttgcc cggcgcccac cccaccgcat cgagtgccat
     3361 cacatcgacc agtatccgct cgaaagcgaa gcggcatacc accggagaac cgatcacatg
     3421 ggaaaacccc gctctcacca actcttcgcg caccgtctca agttgagtaa gcgatgcgat
     3481 cttaaaaaca aggtcgatat ctttggtcgc ccgcatttcc gagaattccg gccggtcggt
     3541 atacagaacg gcgaccgcgc cgcccacgaa cactgcgggc cgcgacaggg aaccgaccag
     3601 cgccgccatt tttttgatat cgtcgatcac gatgctcatg tcagtcgcgc ctccaattcc
     3661 tttaccgcca gttcccgttc ccgtgcacgg ccgacccgaa gcgcgtcgac gagcgcgaga
     3721 agttcgtaca gccgctcgtc ggccaaacag gcgtcgggaa cggtgggata gaggggcgtt
     3781 atcgcctgtc cacgcaccgc gccgtcgata tgaggccaga cgaacgcatc gccgccgccg
     3841 atctcgtcac tgagcggctt ggcgctgtgc gccgtcggaa cgccccgcgt cagggtaccg
     3901 ggaacctgcg ggaagacata gcggatcccg tggatcagga actccttcaa cgcggcgcgc
     3961 atcacggtct tcttgtcggc ggccagcagt ccggccatcg cgctacgctg aaccgattcg
     4021 ctgacctcgc tacgactgat gcgcaggctg tcggcgacat ctttcaggaa ccacggttcc
     4081 ttcatgccgg tgatcttcaa caagaccacg atgtcgagcg gtcgcatacc attgtgttgt
     4141 ttcatggtca cctcaatttc gcaattcgca aaccgcgaat caagaaccat gatagccgct
     4201 cagtaaagat agtcaagtaa aaaattagat aaaataatta gttatcagtc ggccaatcat
     4261 atcgccgcgc ggcggagaag caagagaaac ggtggtggcg ggagcgcggg agatgaatcg
     4321 gtagcggcgg ctacttcttc accggcttgg gagcgggatt gacgggggcg gtcgcggtcg
     4381 gtaatagatc caactttccg agtttgttga agtaatactc agataaaccg aacaggaccc
     4441 cat
//
LOCUS       DAOQUE010000220         4436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_21948_length_4436_cov_3.267975, whole genome shotgun sequence.
ACCESSION   DAOQUE010000220 DAOQUE010000000
VERSION     DAOQUE010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21948_length_4436_cov_3.267975"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="PKH10_07600"
     CDS             <1..1615
                     /locus_tag="PKH10_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28027.1"
                     /translation="IADGVECPDQPCRDTDSDGTPDHRDSDSDDDGIPDLIEGSGDRD
                     DDGLPDYRDTDSDGDGIPDSVECPAQPCVDSEEDGTPDHLDTDSDGDGLSDEQEAALG
                     TDPTKEDSDDDGVSDLAEFSYGSDPLDDTDTIPADDFYVVLPYAPEEEVLRTLDFGTA
                     IQKADILFLLDLSGSMSGEMTNIKNNLTGTIIPGIEAAIPDAAIGLATFEDWRGIHDD
                     TEHPSFDDHIYTVVQTPTADTGTITTAISGLSYLSNGGQEPQIEALYQAATGTGFSGK
                     FDYLYVSAPYHWEATFYPEIPTKNCAGAAGDIGGACFREQTMPIFIMMTDEAFTAAVT
                     NPNIFVWDDTAPYELPHTRDEAIAAMNAIQAKFVGISSFVSLGWNYDPKSDLEAVGVG
                     TGSVDGSSVPFFYEIGDDGAQMSLQIVEAITELTQQVRMDVTTLATPLANPQNVDTTG
                     FIKAVTPLSADPTDGYDTKNDTVFYGVRPGIEVTFEVVFENDVYEPTGPDATLFRAQI
                     AVLGDGALLDTREVLIIVPGKIENSGGPQ"
     gene            1649..2569
                     /locus_tag="PKH10_07605"
     CDS             1649..2569
                     /locus_tag="PKH10_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HNT28028.1"
                     /translation="MSAYAPWMLFGAIVAAIFVIDFFFINRNAHEIRVRESLIVTIAI
                     NIVVALFAFYVRSIKGDEAMLQFFTGYVVEYALSVDNLFVFLVLFSLFKVPERYRHRV
                     LFYGILFAIFARMAFIFLGVAVVNRFHWMLYLLGAFLVYTGVKLFFEKQDDERTVMDG
                     WFFRQVSRFVPLKKELCEHCFFVREEGRWLATPLFMCLLAVEMSDILFAFDSVPAILT
                     ISRDSFVIYTSNLFAILGLRSLFFALSGALRYFRFLNYGLAIILTFIGVKILVEEWWR
                     VPIGLALGFVGLVLGATVLLSLMFPARKEA"
     gene            complement(2573..3427)
                     /gene="lipA"
                     /locus_tag="PKH10_07610"
     CDS             complement(2573..3427)
                     /gene="lipA"
                     /locus_tag="PKH10_07610"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNT28029.1"
                     /translation="MSDHDTPRKKTTVLSPEIHAVKRVLRRHHLHSVCESAHCPNIAD
                     CFGKGTATFLVLDNTCTRDCRFCNISHAEQLAPPDPEEPENIARAVAELGLRYVVITS
                     VTRDDLPDGGAGHLARTIAAVHARSEGTCIEVLTSDMQGDPAALAIIADARPDVFNHN
                     VETVPRLYETVRPQADYRRSLRVITFMKERRLATKSGFMVGLGESDEELRTLLDDLYA
                     AGCDIVTVGQYFRPSHAHLPVDRDLTEEEFEKLRAYGISVGLREIYAGRFVRSSFNAA
                     ELFSATLR"
     gene            complement(3420..>4436)
                     /gene="thiI"
                     /locus_tag="PKH10_07615"
     CDS             complement(3420..>4436)
                     /gene="thiI"
                     /locus_tag="PKH10_07615"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HNT28030.1"
                     /translation="DMTETTSILALIPGISSFSPAQKLPIDASWEDIEQAALPLADRA
                     IAEGRTVFRTTVTRSNKKYPSTSIELQKRLAASVLKARDGRFTVSMTRPNFTLEMEID
                     GDAIYLFSQRIAGTLGLPVGCAGEMLTLLSGGIDSPVAAYLMMRRGARSHYISFHSPP
                     YTTEESMRKLHDLAATLRRFQGEGSRLYIVPFIDLQLLVRARAIESYRTILFRRLMFR
                     LAEHLALRDRYTALVTGESLSQVASQTIENITAIGDAVRTLPVLRPLIAYEKQETIAV
                     ARRIGTFDISIRPCPDSCTAFLPRNPATRGKLDLVHAQEEKLLPDIDALIEAALAGTV
                     TTDV"
BASE COUNT          786 a         1318 c         1451 g          881 t
ORIGIN      
        1 catcgccgat ggtgtcgaat gccccgacca accgtgccgg gacaccgatt cggacggcac
       61 gcccgaccac cgcgacagcg acagcgacga tgacggaatc cccgacctta tcgagggttc
      121 gggcgatcgt gatgacgacg gtcttcccga ctatcgcgat accgacagcg acggggacgg
      181 tatccccgac agcgtcgagt gccccgcgca gccctgcgtc gattctgagg aagacggcac
      241 gcccgaccat ctcgacaccg acagtgatgg tgacggtcta tccgacgagc aagaggcggc
      301 gcttggcacc gacccgacca aggaggatag cgatgacgac ggggtgagcg atctcgccga
      361 gttctcgtac gggtccgatc cgctcgacga tacggacacc atacccgccg acgatttcta
      421 tgtcgtgctt ccctatgccc ccgaggagga agtgctccgg acgctcgatt tcggcaccgc
      481 catccagaag gccgacatcc tcttcctgct cgacctctcg ggctcgatga gcggcgagat
      541 gacgaacatc aagaacaacc tcaccggcac gatcataccc ggtatcgagg cggcgatccc
      601 cgacgccgcg atcggtcttg ctaccttcga ggactggcgc ggcatccacg acgacacgga
      661 acatccgtcg ttcgatgacc acatctacac ggtcgttcag acgccgaccg ccgataccgg
      721 cacgataacg acggcgatat cggggctctc ctatctctcg aacggtggtc aggaaccgca
      781 gatcgaggcg ctctatcagg cggcgaccgg caccggtttc tcggggaagt tcgattacct
      841 ctatgtcagt gcgccgtacc actgggaagc gaccttctac ccggagatac ctaccaagaa
      901 ctgcgcggga gcggcgggtg acatcggcgg cgcctgtttc cgcgagcaga ccatgccgat
      961 attcatcatg atgaccgacg aggccttcac tgcggcggtg acaaatccga atatctttgt
     1021 gtgggacgat acggcgccct acgaactgcc ccacactcgc gacgaggcga tagcggcgat
     1081 gaacgcgata caggcgaagt tcgtcggcat cagttctttc gtgtcgctcg gctggaacta
     1141 cgatcctaag tccgacctcg aagcggtcgg cgtcggtacc ggatcggtcg atggctcgag
     1201 tgttcccttc ttctacgaaa taggcgatga cggtgcgcag atgtcgctcc agatcgtcga
     1261 ggcgatcacc gaactgaccc agcaggtacg gatggatgtc acgacgctcg cgacgccgct
     1321 cgccaatccg cagaacgtag atacgaccgg gttcatcaag gcggtgacgc cgctgtccgc
     1381 cgaccctacc gacggctacg ataccaagaa cgacacggtc ttctacgggg tgagaccggg
     1441 tatagaggtg accttcgagg tggttttcga gaacgatgtc tatgaaccga ccggtcccga
     1501 tgcgacgctg ttccgcgccc agatagcggt gctcggcgac ggcgcgcttc tcgatacccg
     1561 cgaggtgctc atcatcgtgc cgggcaagat agagaacagc ggcggcccgc agtgaatgtc
     1621 ttgagccacc attgagagag gtcccttgat gtccgcctat gccccgtgga tgctattcgg
     1681 cgcgatagtc gccgcgatat tcgtcatcga tttctttttc atcaaccgta acgcccacga
     1741 gatacgggtc cgcgaatcgc tcatcgtcac catcgccatc aacatcgtcg tagccctgtt
     1801 cgccttctat gtccgttcga tcaaaggcga cgaggctatg ctccagttct tcaccggcta
     1861 tgtcgtcgag tatgcgctgt cggtcgacaa cctgttcgtg ttcctcgtgc tgttctcgct
     1921 gttcaaggtg ccggagcggt accgccaccg cgtgctgttc tacggcatcc tcttcgcgat
     1981 attcgcacgg atggccttca tcttcctcgg ggtcgcggtc gtcaaccggt tccactggat
     2041 gctctacttg ctcggcgcgt tcctcgtcta taccggcgtc aaactcttct tcgagaagca
     2101 ggatgacgag cgcaccgtca tggatggctg gttctttcgg caggtgagcc gcttcgtccc
     2161 gctcaagaag gaactctgcg agcactgctt cttcgtgcgc gaggagggga gatggctcgc
     2221 gacgccgctt ttcatgtgtc tgctcgccgt cgagatgagc gacatcctct tcgccttcga
     2281 ctcggtgccg gcgatactca cgatatcgcg cgacagtttc gtcatctata catcgaacct
     2341 ctttgcgata ctggggctcc gatcgctctt cttcgcgttg tcgggggctc tgcgctattt
     2401 ccggttcctc aactacggac tcgcgatcat cctcaccttc atcggggtga agatactcgt
     2461 ggaggagtgg tggcgggtcc ccatcgggct cgcgctcggc tttgtcgggc tcgtgctggg
     2521 cgcgacggtg ctcctttcgc tcatgttccc cgcccgcaag gaggcgtgac cgctaccgga
     2581 gcgttgccga aaagagttcg gcggcgttga aggaactgcg gacgaaccgt ccggcgtaga
     2641 tctcgcgcag gccgacggag atgccgtatg cgcggagctt ctcgaactct tcctccgtca
     2701 gatcgcgatc gaccggcagg tgtgcgtggg aggggcggaa gtactgcccg acggtgacga
     2761 tgtcgcatcc ggcggcatac aggtcgtcca gcagcgtgcg cagttcctcg tcgctttccc
     2821 cgagcccgac catgaacccc gattttgtcg cgagcctccg ttctttcatg aaggtgatga
     2881 cccgcaggga gcggcggtag tcggcctgcg gacggaccgt ctcatagagg cgtggcactg
     2941 tttcgacatt gtgattgaac acatcgggac gggcgtcggc gatgatcgcg agcgcggcgg
     3001 ggtcgccctg catatcggag gtgagcacct cgatacatgt tccttcggac ctcgcgtgga
     3061 cggcggcgat ggtccgagcg aggtgcccgg ccccaccgtc gggaaggtcg tcgcgggtca
     3121 cagaggtgat gacgacatag cgcagtccca gttcggcgac ggcgcgggcg atattctccg
     3181 gctcctcggg gtcgggtggg gcgagttgtt cggcgtgtga gatgttgcag aaacggcagt
     3241 cgcgggtgca ggtgttgtcc agtacgagaa aggtggcggt cccttttccg aagcagtcgg
     3301 ctatgttcgg acagtgggcc gattcgcaga ccgaatggag atgatggcgg cgtagcaccc
     3361 gtttgacggc gtgtatctcg ggcgacagga ccgtcgtctt cttgcggggg gtatcatggt
     3421 cagacatcgg tcgtcacggt gccggcgagg gccgcttcga tgagtgcgtc gatgtcgggc
     3481 aggagttttt cctcctgtgc gtggacgagg tcgagtttgc cccgggtcgc cgggttgcgt
     3541 gggaggaagg cggtgcagga gtcggggcag gggcgtatcg agatgtcgaa ggtgccgatg
     3601 cgtcgcgcga ccgcgatggt ctcctgtttt tcgtaggcga tgagcgggcg gagcaccggg
     3661 agcgtccgca ccgcgtcgcc gatggcggtg atgttctcga tggtctggct tgctacctgc
     3721 gagagcgatt caccggtgac aagcgccgta tagcggtccc gcagggcgag gtgctcggcg
     3781 aggcggaaca tgagtcgccg gaacaggatg gtgcggtacg attcgatggc gcgcgccctg
     3841 acgaggagtt gcagatcgat gaacggcacg atgtagagcc gcgaaccctc gccctgaaag
     3901 cggcggagcg tcgcggcgag atcgtgcagt ttgcgcatgc tctcctcggt cgtgtaggga
     3961 ggcgaatgga aactgatgta gtgcgaccgg gcgccgcgcc gcatcatgag gtacgcggcg
     4021 accggcgaat cgatgccgcc cgacaggagc gtgagcatct cgccggcgca accgaccggg
     4081 agcccgagcg tccccgcaat ccgttgcgag aaaaggtaga tggcgtcgcc gtctatctcc
     4141 atctcgagcg tgaagttcgg tcttgtcatc gagacggtga agcgcccgtc gcgcgccttg
     4201 agtaccgacg cggcgagccg tttctgcagt tcgatggagg tggagggata cttcttgttc
     4261 gaccgggtga cggtggtgcg gaacacggtg cgcccttcgg cgatggcgcg gtcggcgagc
     4321 gggagcgccg cctgctctat gtcctcccac gacgcgtcga tgggcagttt ctgcgcgggc
     4381 gagaaactcg atatgccggg gatgagggcg aggatggagg tcgtctcggt catgtc
//
LOCUS       DAOQUE010000221         4420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_22039_length_4420_cov_5.118213, whole genome shotgun sequence.
ACCESSION   DAOQUE010000221 DAOQUE010000000
VERSION     DAOQUE010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22039_length_4420_cov_5.118213"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1619)
                     /locus_tag="PKH10_07620"
     CDS             complement(33..1619)
                     /locus_tag="PKH10_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT28031.1"
                     /translation="MKQILFLATVTILLLACKEGATPVSVPDIDHLLPPPPTQETAKG
                     KSVDEKTLPVAEAEKSTFEPVDISACVNMLEKKGLITKSDYEYRSKGVGGSNTVKYST
                     HLRGYLKLFSYSRQFIPDKKVESSDDVALNFVFGREEIHALGECLEKNPQNHKRNKIY
                     QFKIALADNGKVKLIKNTNIPHGCLKEYLEKVTFPELWLKKNEKYRRLEITVKIKKEY
                     EYTYPFLIAAEKKERNVQKCLIEKCKSRKDVLNEMLILSAELGDTENIKTLVDAGAEV
                     NAPSRVWMFYGSALFSAALNGQTEAIHTLLEKGADINATNGLGQTILSKLKNSCERIC
                     KDQSCPCQHEEMIKYLLAHGAQAEKWYFPPNTIDPFWNSVASKQLNSMKEPPLYDQAI
                     KEGQESYRLLWLRSFHPPVIIKIEKNAEAYSLYLKVRDESCPGKLAVDKKVDLSEKDW
                     LAFKESLTKANYWKLSEDKSPQGTDGSEWILEAKTVDRYHVVIRWSPDSRGESRGTKE
                     FAELGLFILKLTGQTFEPIY"
     gene            complement(1641..3671)
                     /gene="ligA"
                     /locus_tag="PKH10_07625"
     CDS             complement(1641..3671)
                     /gene="ligA"
                     /locus_tag="PKH10_07625"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNT28032.1"
                     /translation="MKILTQRDYEELVQELSRASTAYHTLDAPVMSDVDYDMKMRELR
                     AIETEHPDWKVKHSPTMRVGGPLRDGFKKVTHPRRMTSLEDAFTAEEVRAFFERLKME
                     LPRGFIVEQKIDGLAVSILYRNGRFVQAATRGDGEIGEEVTQNVATVKNIPLMLDAAQ
                     VPAEFEVRGEVYLPEASFQKLNADRLAAGDAPFANPRNAAAGSLRQLDPKVTAGRDLK
                     FFAYGIGMDGAPPVATQSALHDFLRRLGFSTPDFTLVDTIDHLAPVIEKTIAARGELG
                     YDIDGLVIKLDSFAEQEIAGFLTRTPRWAIAYKLPAQEKSTILEKVDWQVGRTGVVTP
                     VARLKKIELMGTMVENATLHNMEEIERKGLYLGAEVFVRRAGDVIPEVVSTANATYSP
                     QELAAFSLAGDNVPSFVIIPPKECPECHTPLTRDADKVALRCPNPACPARVVESIIHF
                     ASRKGFNIDGLGDKQVEFFHEKGWLKSVADIFRLKDWSMWLMGQKGWGEKSVYNLLDA
                     IEKAKTVKFQNMLFALGIPEVGEFMAGELAKRFTLDELMRATEAELLTIDGVGGVVAQ
                     AIVAYFGNEVNRREIERMRADGLNIVYPEKVAFDATLAGLVFVITGELSKPRDEFKQL
                     IESKGGRVTGSVSKKTSYVLAGIDPGSKLDKAKELGIPVLDEAGFNALIAGR"
     gene            complement(3668..3868)
                     /locus_tag="PKH10_07630"
     CDS             complement(3668..3868)
                     /locus_tag="PKH10_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HNT28033.1"
                     /translation="MECANRQKNISRCTCTYEGCPRHGICCECIAYHNRMGEFPACFF
                     SKEKERTYDRTYENLVRDREGR"
     gene            4030..>4420
                     /locus_tag="PKH10_07635"
     CDS             4030..>4420
                     /locus_tag="PKH10_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28034.1"
                     /translation="MKHLRNGLVLFFLCTTFLLGAQEPSETDAPAATGPAPASEAVVA
                     ESAPPAESPVPALFAAEEQVPFDKAGSIMAIDLNLRTRYGLFPDLPGFRQALLFKTSR
                     GFELALTIEENGSIVDRRMAITEQQVTD"
BASE COUNT          899 a         1230 c         1184 g         1107 t
ORIGIN      
        1 aaagtcagcc gtcagccgaa agactattaa agtcagtaga taggttcgaa agtttgcccg
       61 gtgagcttca aaatgaaaag tcctaattcg gcaaattctt ttgtgccacg gctttcgcca
      121 cgactgtcag gagaccaacg gataaccaca tggtatctgt caacggtctt cgcttcgagt
      181 atccactcag atccgtcggt gccctgtgga gatttatctt ctgatagttt ccaatagttg
      241 gctttggtta aactttcttt gaatgcaagc caatcttttt ccgaaagatc aactttcttg
      301 tcaaccgcca atttcccggg gcaagattca tcgcgcactt tgagataaag agaatatgct
      361 tcagcgttct tctcaatctt gattattacc ggtggatgaa aacttcttag ccacaagagc
      421 cgataacttt cttgtccttc ctttattgct tggtcataca gggggggctc tttcatgcta
      481 ttaagttgct tggaagccac gctgttccaa aaaggatcta tggtattggg aggaaaatac
      541 catttttcgg cttgggcgcc atgcgccaag aggtatttga tcatttcctc atgttggcaa
      601 gggcacgatt gatccttgca gattctttcg catgaattct ttaacttaga gagtatcgtt
      661 tgccccaatc cattggttgc atttatgtcc gctccttttt ccagaagagt gtggatggct
      721 tcagtttggc cattcaatgc cgcagaaaat aaggcagaac cataaaacat ccataccctt
      781 gacggtgcat tgacttctgc ccctgcgtct accagcgtct tgatgttttc ggtgtctcca
      841 agttctgcgg aaaggatcaa catctcatta aggacatctt tgcggctttt gcatttctca
      901 ataagacact tctgaacatt tctttctttc ttttctgccg cgatcaggaa gggataggta
      961 tattcatatt ctttcttaat ttttaccgtt atttccagcc tcctgtactt ctcgttcttt
     1021 ttcaaccata gttccgggaa agttaccttt tcaagatact ctttcaggca accatggggt
     1081 atgtttgtgt tctttataag cttaactttg ccattatcgg ccaaagcgat tttaaattgg
     1141 tatatcttat ttctcttatg attctgtgga tttttctcta ggcattcacc aagagcatga
     1201 atctcctcgc ggccaaaaac aaaattaagg gccacatcgt cactgctctc aactttcttg
     1261 tcaggaatga attggcgcga atatgaaaat aattttaagt atccccgaag atgggttgaa
     1321 tacttgaccg tattcgaacc gcctaccccc ttggatcggt attcataatc cgattttgtg
     1381 ataagaccct tcttttcaag catattgaca caggccgaaa tgtccaccgg ctcaaaagtc
     1441 gacttttcag cttcggctac aggaagagtt ttctcatcga cgctttttcc tttggcagtc
     1501 tcttgtgtcg gaggcggtgg caagaggtgg tcaatatcag gaacagatac cggcgttgct
     1561 ccctctttgc aagcgagaag aagaattgtt accgtggcga ggaaaagaat ttgtttcata
     1621 cgcccccttc atctacaaaa tcatctgccc gctatgagcg cgttgaagcc cgcttcgtcg
     1681 aggaccggga tgccgagttc tttggctttg tcgagtttcg agccggggtc tattcccgcc
     1741 agcacataac tcgttttttt cgacaccgat ccggtgacgc ggcccccttt cgattcgatg
     1801 agttgcttga actcgtcgcg cggtttcgag agttcaccgg tgatgacgaa gacaagtccc
     1861 gcgagcgtcg catcgaacgc caccttttcg gggtagacga tgttgaggcc gtcggcgcgc
     1921 atccgctcta tctcgcggcg gttcacctcg ttgccgaagt aggcgactat cgcctgcgcc
     1981 accaccccgc caaccccgtc gatggtcagc agttccgcct cggtcgcccg catcaactca
     2041 tcgagcgtga agcgtttcgc cagttcgccc gccatgaatt cgcccacctc ggggatgccg
     2101 agggcgaaga gcatgttctg gaacttgacc gtctttgctt tttctatcgc gtcgaggaga
     2161 ttgtagaccg acttctcacc ccatcctttc tgccccatga gccacatcga ccagtccttc
     2221 agccggaaga tgtcggcgac cgacttgagc cacccttttt cgtggaagaa ctcgacctgc
     2281 ttgtcgccga gtccgtcgat gttgaatccc ttacgcgagg cgaagtgaat gatgctctcg
     2341 accacgcggg cggggcaggc ggggttggga cagcggagcg ccaccttgtc ggcatcgcgg
     2401 gtgagaggcg tatggcattc ggggcattct ttaggcggaa tgatcacaaa agagggcaca
     2461 ttgtctccag caagggaaaa ggcggcaagt tcttggggcg agtaggtcgc attcgccgtc
     2521 gagacgacct ccggtatcac gtcgccggcg cgccgcacga agacctcggc gccgagataa
     2581 agccccttgc gttcgatctc ttccatgttg tggagcgtcg cgttctcgac catcgtcccc
     2641 atgagttcta tctttttcag ccgtgcgacc ggggtcacga ccccggtgcg gcctacctgc
     2701 cagtcgacct tttcgaggat ggtgctcttc tcctgcgccg ggagtttgta ggcgatcgcc
     2761 cagcgcggcg tccgggtgag aaagccggcg atctcctgtt cggcgaagga gtcgagtttt
     2821 atcacgagcc cgtctatgtc gtagccgagt tccccgcgcg ccgcgatggt cttttcgatg
     2881 accggcgcaa ggtggtcgat ggtgtcgacg agggtgaagt cgggggtcga gaaaccgagg
     2941 cggcgcagga agtcgtggag cgccgactgg gtggcgaccg gcggcgcgcc gtccataccg
     3001 atgccgtagg cgaagaattt gaggtcgcgc cccgccgtca ccttcgggtc gagttgccgc
     3061 agacttccgg cggcggcgtt gcgcggattg gcgaagggcg catcaccggc ggcaagccgg
     3121 tccgcattca gtttctggaa actcgcctcg gggaggtaga cctcgccgcg cacctcgaat
     3181 tcggccggca cctgtgccgc gtcgagcatg agcgggatat tcttcaccgt cgcgacattc
     3241 tgcgtcacct cttcgccgat ctcgccgtcg ccgcgcgtag cggcctgcac gaaccggccg
     3301 ttgcggtaga ggatcgacac ggcaagaccg tctatcttct gctcaactat aaagcctctc
     3361 ggcagttcca tcttgagccg ctcgaagaaa gcgcggacct cctcggcggt gaaggcatcc
     3421 tccagcgagg tcatacggcg gggatgggtc acttttttga acccgtcgcg caacggtccg
     3481 ccgacacgca tcgtaggaga atgcttgacc ttccagtcgg gatgctctgt ttcgatggcg
     3541 cgcagttcgc gcatcttcat atcgtagtcg acatcgctca tcacgggagc gtcgagcgtg
     3601 tggtaggcgg tcgatgcgcg gctgagttct tggaccagtt cttcgtagtc gcgctgcgtg
     3661 agtatcttca tcgcccctcg cggtcgcgca cgaggttctc gtaggtccgg tcgtaggtcc
     3721 gctctttctc cttggagaag aaacaggcgg ggaattcgcc catgcggttg tgataggcga
     3781 tgcactcgca acagataccg tggcgcggac acccctcata ggtgcaggtg caccgcgaga
     3841 tgttcttttg tctgttcgca cattccatga gaggaacctc ccgaaaacga ccctgtcctc
     3901 ctggttgtat cagatgcccg cgatcaagtc aatgggagcg ccgagcgaca caaaatagtt
     3961 gcaaagccct gaacgacgct ataccatcgt atccgcagaa aataactgcc gacggggttc
     4021 gtgatggaga tgaaacactt acggaacggt ctggttttgt tctttctctg caccaccttt
     4081 ctgctgggtg cgcaggaacc gtccgaaacc gatgccccgg cggcgaccgg acccgcgccc
     4141 gcctccgaag cggtcgtggc agagtctgcg ccccccgccg aatcgccagt cccggcgctc
     4201 tttgccgccg aggaacaggt gcccttcgac aaggcgggat cgatcatggc gatcgatctg
     4261 aacctccgca cccgctacgg actcttcccc gacctgcccg gtttccggca ggcgctcctc
     4321 ttcaagacat cgcgcggctt tgaactggcg ctcacgatcg aggagaatgg ctctatcgtc
     4381 gaccgacgca tggcgataac cgaacaacag gtgaccgatc
//
LOCUS       DAOQUE010000222         4417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_22059_length_4417_cov_4.300779, whole genome shotgun sequence.
ACCESSION   DAOQUE010000222 DAOQUE010000000
VERSION     DAOQUE010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22059_length_4417_cov_4.300779"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PKH10_07640"
     CDS             complement(<1..843)
                     /locus_tag="PKH10_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28035.1"
                     /translation="MKAIFTACALIIGAMLMTACGGYEDQYLNKLYSKESRTWAINQI
                     IKEKDKIENKEKAIKKLLSLDQTPDIPMALGAVGAIGDPIAVPRIGEIVDGALKVLNV
                     RNLKTLESAAMALGMIGDPSAAPILGKYFTIQTPEGLAAGQREKPESVAKRAAIEALA
                     KMPEAGKQFLPQILQVFASKYEDFGTKYTTAGVIGEFGDPSAVAPLVTALFYEEQGYS
                     LFPEARKSIIRLGKYAENAIMKAYKNEDVSVNEILDKYREKALKQFCPEYLDPEKKKK
                     GECSK"
     gene            1056..1907
                     /gene="speD"
                     /locus_tag="PKH10_07645"
     CDS             1056..1907
                     /gene="speD"
                     /locus_tag="PKH10_07645"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNT28036.1"
                     /translation="MKLYGFNNLTKTLSFNIYDVCYAMTREDRRAYIQYIDEEYNSDR
                     LTGILEQVSEIIGSNVLNISKQDYDPQGASVVVLISEEKVVQGGKLEGSIYEGTTDCA
                     DNGEGGDNGDNGEGGDVTMHLDKSHLTVHTYPESHPDSGISTFRVDIDVSTCGRISPL
                     KALNFLIDNFESDIINIDYRVRGFTRVLDDRKIFIDHKIHSIQNFIHPDTLDAFHCVD
                     LNVYQDNIFHTKMILRDFDLDNYLFGVSKRQINSMEKRKIKQRLTREMNEIFYGRNLP
                     SMPKLFI"
     gene            complement(1908..2042)
                     /locus_tag="PKH10_07650"
     CDS             complement(1908..2042)
                     /locus_tag="PKH10_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28037.1"
                     /translation="MVMQNEWWQGAVCVELSFMDAGRPGYHDQGDEDEGKKKCAGTHR
                     "
     gene            2091..3698
                     /locus_tag="PKH10_07655"
     CDS             2091..3698
                     /locus_tag="PKH10_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HNT28038.1"
                     /translation="MFGYLPYWTNDTRHLRYSLLSDIAFFSCELGVNGALGDCHGWPA
                     AAPIAEAHGYGVKVHLVVTGFDKATVLDLAKSDGQKNTFFAETYAKVHDAGADGLNID
                     FEISSGDDSTVIAQFFNDLADYFHSRDASLEVSAALWAVDWQDTFSIGSMTGVDLFFV
                     MAYDYHWKGGDPGPVSPLISQSPWPTGGICVTRSYDDYAAKLGDTGLERLILGFPYYG
                     YDWPTTSDAVPGTRTANGVAALYENIDMDGAIYDTASRTVYRAYQDGGWHQRWFDTPA
                     TFAEKLAFVNDNPSGGAGMWALNYDGASDEFWHEIARAFTDDVPGPPDDPITVTAFPF
                     AHDGNTYRYRSDSFDAYICDNQPDSAGINEEGPETVYELTIDTAGTLTATLTARAGEG
                     ATDREDLDLHLLWTLSSGDCLTRAHVSFNEHLPAGVYYLVVDSYVTADGVRKGGPYRV
                     ELSFSPDETPDDGMPDTDTPNGDDTDELLTDGFGTDDATTPNDTDLPAGSDTADDSET
                     TTGDEPLSDGTTVPPPPADGCGCSLPG"
     gene            3799..>4417
                     /locus_tag="PKH10_07660"
     CDS             3799..>4417
                     /locus_tag="PKH10_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28039.1"
                     /translation="MKMKHKILYAAGVVAMLALLAGDIIFFSRLDLIKDDASIINLSG
                     TVMGGIQRAIGTELGGRNADPLLAETDKALTLLFDHARSTGNGELAAATDEIKKEWAR
                     LKELLLVHRAEASPEIRENIIIIGERLWANTAETLHIVQRDTERRMKLFYLLIPALIA
                     MVIILVSCSLMTRLYLRLRIRHLSTHDRTTDTLNPPAFAYILQQHL"
BASE COUNT          919 a         1426 c         1197 g          875 t
ORIGIN      
        1 cttggaacac tcgcccttct tctttttctc agggtcgaga tattcggggc agaactgctt
       61 gagcgccttt tcacggtatt tgtcgagtat ctcgttcacc gagacatcct cgttcttgta
      121 agccttcatg atggcgttct cagcgtactt gccgagccgg atgatcgact tgcgcgcctc
      181 ggggaacagg ctgtagccct gttcctcgta gaacaacgcg gtgacgagcg gagcgaccgc
      241 ggaaggatcg ccgaactcgc cgatgacgcc cgccgtggtg tatttggtgc cgaagtcctc
      301 gtacttcgag gcgaagacct gcagtatctg cggcaggaac tgcttgcccg cctcgggcat
      361 cttggcgagc gcttcgatgg cggcgcgctt ggcgaccgac tcgggctttt cacgctggcc
      421 ggcggcgagt ccttcggggg tctggatggt gaaatacttg ccgagtatcg gcgcggcgct
      481 cgggtcaccg atcatgccca gcgccatggc ggccgattca agcgtcttga gattgcgcac
      541 attgagcacc ttgagcgccc cgtcgacgat ctcgccgatg cgcgggacgg cgatcgggtc
      601 tccgatcgcg ccgacggcgc cgagcgccat cggtatatcg ggggtctgat cgagactcag
      661 gagtttcttg atggctttct ccttgttttc gatcttatcc ttttccttga tgatctggtt
      721 gatcgcccaa gttcttgatt cttttgaata aagcttgttc aggtactggt cctcgtaacc
      781 gccgcacgcg gtcatgagca tcgcgccgat gatcagggcg cacgcggtga agatcgcttt
      841 cataagacac ctctcattag gtcaataaag ccgacatttc agaggcactg taacagaaaa
      901 aagacaaaat tcaatctttt tcgacatcgt tttttttaat cgttttttgt tgaaaaaaag
      961 gcgccgccgt ctatgctgtc gctggtatcg atcgtttttt cgacactaca caggagggtc
     1021 ccgtgaaggt caacaagatc gccccccaca gaaaaatgaa actctacggc ttcaacaacc
     1081 tcaccaaaac tctttccttc aacatatacg atgtctgcta cgcgatgacc cgcgaggacc
     1141 gccgcgccta catccagtac atcgacgagg aatacaactc cgatcgcctt accggcatcc
     1201 tcgagcaggt atccgagatc atcggctcaa atgtcctcaa catctcgaag caggactatg
     1261 atccgcaagg ggcgagcgtc gtggtgctca tctcggagga aaaggtggtg cagggaggga
     1321 aactagaggg ttccatctac gaaggaacga ccgactgcgc cgacaacggc gaaggcggcg
     1381 ataacggcga caacggtgag ggcggcgatg tgacgatgca cctcgacaag agccatctca
     1441 cggtccacac ctaccccgaa agccaccccg acagcggcat atcgaccttc cgcgtcgata
     1501 tcgacgtgtc gacctgcggc cggatatctc ctctgaaggc gctcaacttc ctcatcgaca
     1561 acttcgagtc ggacatcatc aacatagatt accgggtccg cgggttcacg cgggtccttg
     1621 atgatcgcaa gatattcatt gaccacaaga tacactcgat acagaacttc atccatcccg
     1681 acacgctcga cgccttccat tgtgtcgacc tcaatgtcta tcaggataac atcttccaca
     1741 ccaagatgat cctgcgcgat ttcgatctcg acaactatct gttcggcgtc tcgaagcggc
     1801 agataaactc tatggagaaa cgcaagatca aacagcggct cacccgcgag atgaacgaga
     1861 tattctacgg tcgcaatctc ccctcgatgc cgaaactgtt catctagtca tcgatgcgta
     1921 ccggcgcatt tcttcttccc ctcatcttcg tccccctgat cgtgatagcc gggacgaccg
     1981 gcgtccatga acgagagctc gacgcacacc gccccctgcc accactcgtt ctgcatgacc
     2041 atgccgccgt cgtcccgcgc ggaacgcccg ccccgatact gacaaagcgg atgttcggct
     2101 atctccccta ctggacgaac gacacgagac acctgcgcta ttcgcttctt tccgacatcg
     2161 ccttcttctc gtgcgaactg ggcgtgaacg gcgcgctcgg cgactgccac ggctggcccg
     2221 ccgccgcccc gatcgccgag gcgcacggct acggcgtaaa ggtccatctc gtcgtgaccg
     2281 ggttcgacaa ggcgaccgta ctcgacctcg cgaagagcga cgggcagaag aacacctttt
     2341 tcgcggagac atacgccaag gtgcacgatg cgggggccga tggactgaat atcgacttcg
     2401 agatatcgag cggcgacgac agcaccgtca tcgcgcagtt cttcaacgac ctcgctgact
     2461 atttccacag ccgcgacgca tcacttgagg tgtcggctgc tctctgggcg gtcgactggc
     2521 aggacacctt ctccatcggc tcgatgaccg gggtggacct tttcttcgtc atggcctacg
     2581 attaccactg gaaggggggc gatccgggac cggtcagccc cttgatatcg caatcgccgt
     2641 ggccgacggg aggcatttgt gtgacgcggt cctacgacga ctacgccgca aaactgggcg
     2701 atacgggact cgaacgattg atcctcgggt tcccctacta cgggtacgac tggccgacga
     2761 cctccgacgc ggtgccgggg acgaggaccg ccaacggggt cgcggcgctg tacgagaaca
     2821 tcgacatgga cggcgcgatc tacgataccg cgtcgaggac ggtctaccgc gcctatcagg
     2881 acggcggctg gcatcaacgc tggttcgaca cccccgccac ctttgccgag aaactcgcct
     2941 tcgtcaacga caatccttcg ggcggcgccg ggatgtgggc gctgaattac gatggcgcga
     3001 gcgatgagtt ctggcacgag atagcccgcg ctttcaccga tgatgttccc ggtccgcccg
     3061 atgacccgat caccgtgacc gcctttccct tcgcccacga cggcaacacc taccgctacc
     3121 gttccgattc gttcgacgcc tacatctgcg acaaccaacc ggacagcgcc gggataaacg
     3181 aggagggccc cgaaacggtc tacgaactga cgatcgatac cgccggtacg ctgaccgcga
     3241 cgctcaccgc cagagcaggc gaaggcgcga ccgaccgcga ggatctcgac ctgcatctcc
     3301 tgtggacgct ctcctccggc gactgcctca cccgcgccca tgtgtcgttc aacgaacacc
     3361 ttccggcggg cgtctactac ctcgtcgtcg attcgtatgt cactgccgac ggtgttcgca
     3421 agggcggccc ctaccgcgtc gagctgagct tctctcccga cgaaacaccc gatgacggca
     3481 tgcccgacac cgataccccc aacggcgacg acaccgatga actcttgacg gacggtttcg
     3541 ggaccgacga cgccacgacg ccgaacgata cggacctccc cgccggatcg gacacggccg
     3601 acgacagtga gacaacgacc ggcgacgaac ctctctccga cggaacgacc gtacccccac
     3661 cgcccgccga cgggtgcgga tgctcactcc ccggttaagg taactcgttc attccccgaa
     3721 caatttgttg acatgacccc ggaactttgc taacaatatg cgtcgggtcc ttttgcggtt
     3781 catcagggga gtggcgccat gaagatgaaa cacaagatcc tctacgcggc gggcgtagtg
     3841 gccatgctgg cccttctcgc cggggacatc atcttcttct ccagacttga tctcatcaaa
     3901 gacgacgcct cgataatcaa cctctccgga acggtgatgg gcggcatcca gcgcgccatc
     3961 gggaccgagc tcggcggccg gaacgccgac ccgctcctcg ccgagaccga caaagcattg
     4021 accctcctgt tcgatcatgc gcggtcgacc ggcaacggcg aactcgccgc agcgaccgac
     4081 gagatcaaga aggaatgggc gcgcctgaag gagttgctcc tcgtccaccg cgccgaggcg
     4141 tcgccggaga tacgcgagaa tatcatcatc atcggcgaac ggctctgggc gaacaccgcc
     4201 gagacgctcc atatcgtaca gcgcgatacc gaacggcgca tgaaactctt ctatctcctg
     4261 atccccgcgc tcatcgcgat ggtcatcatc ctggtatcct gttctctcat gacccggctc
     4321 tatctgcggc tccggatacg ccatctttcg acacacgacc ggaccaccga cacactcaac
     4381 ccccccgcct tcgcctacat cctgcagcag catctcc
//
LOCUS       DAOQUE010000223         4416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_22065_length_4416_cov_3.792479, whole genome shotgun sequence.
ACCESSION   DAOQUE010000223 DAOQUE010000000
VERSION     DAOQUE010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22065_length_4416_cov_3.792479"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2254)
                     /locus_tag="PKH10_07665"
     CDS             complement(<1..2254)
                     /locus_tag="PKH10_07665"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNT28040.1"
                     /translation="MLHIFGAAEHNLKHIDVEIPHNAFTVVTGVSGSGKSSLVYDTIF
                     HESMRRFMGTFSTYTRQYLRKVHRPEVKEITGLPPAVSVDQRSGVTSPRSTVGTLSGL
                     YDLLRLLFARFGNAPEGVRPNRSLFSFNSPEGACPICGGLGVEDSLDLDKLIADPTKT
                     LRGGAFVITTPSGYIMYSQVTMAVLDEVCRAHGFSVDIPWRDLTDEQKRIVLYGSEKI
                     KIPYGKHPLESRMKWTGIKAKPREEGYYRGILPVMEEILKRDRNASILRFVRSVPCAA
                     CHGSRLNETARSVTVAGRSIAQCADMTVKEFAAWCAEFAAVQRHEALVAITAAVARQA
                     RHFTALGIDHLPLARGAASLSGGELQRVRLATQLENRIRGILYILDEPSAGLHPADHA
                     RLMDILTGLRDAGNTVVAVDHNLVAARHADHLIDLGPGPGDAGGEILFAGAPADILNP
                     AYREKSPTARALVQEKEGYPKPEATVPDNANYFWVRGAAKNNLRSVNASFLHKGFTVV
                     TGVSGAGKSSLVECLVEQLEALPSAERPVAAVRFIDQSPIGRNPRSNPATYTKLSDEL
                     RKRFAALPAATERGLTASHFSFNLTGGRCEACEGAGVRELGLHFLGRSEVVCDVCNGA
                     RFSEKVREVTLNGKNIAEVLDLSISDAARFFADDRPLARYLDILCELGLGYITLGQPA
                     TTLSGGEAQRLKLAAALAKKGKGNDLYIFDEPTAGLHPADVALFAEAVGRLIARGNSV
                     IAVTHDLRLIG"
     gene            complement(2269..2700)
                     /locus_tag="PKH10_07670"
     CDS             complement(2269..2700)
                     /locus_tag="PKH10_07670"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HNT28041.1"
                     /translation="MRSTLFAIIIFAFALTAAQATTTLTVKVWGLRKQGQVFVYLYNQ
                     DDGFPTSPEKAFKTITLPVRESDTLFAKFEGLAPGDYAVAVMHDENGNGQIDTNWIGI
                     PKEGLGVSNNAKGVMGPPKFKDAKVTVPAGKKELSIPVKYR"
     gene            2815..3246
                     /locus_tag="PKH10_07675"
     CDS             2815..3246
                     /locus_tag="PKH10_07675"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNT28042.1"
                     /translation="MSDTHSDICREGIVKGYADGRVRVLITVQSACASCHAKGACTSL
                     DMSEKVIDARADGPFEIGEKVKVAVAGRYGWTAVLYAFVLPMVILFTLFFTCLSTGSG
                     ETQAALSGLAGIAIYFLLIRIFRDRLFGRMEFHVERITNCQ"
     gene            3265..4143
                     /locus_tag="PKH10_07680"
     CDS             3265..4143
                     /locus_tag="PKH10_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNT28043.1"
                     /translation="MILYPVIMLGAVAGIAAILLFLTAKKFHVEEDPRIDKVAELLPG
                     ANCGGCGYPGCRGLAEAIVKAADKGDIGGLSCPPGGSAAMKAIGEVLGMAVAESEPMV
                     AVLRCGGTHEKAPRTLKYDGAPKCVIAHTVFAGEKGCPNGCLGLGDCVTVCAFGAMYM
                     DEKTGLPVIDPAKCVACGACVKACPRKIIEMRPKGRKDRRVWVNCVNTEKGAAAMKYC
                     KAACIGCGKCVKECPEKVQAITLQNNLAYIDPKKCIACGKCVLVCPTKAILATFEPPK
                     PKPVESQSAEAPKATV"
     gene            4179..>4416
                     /locus_tag="PKH10_07685"
     CDS             4179..>4416
                     /locus_tag="PKH10_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HNT28044.1"
                     /translation="MNLKTFRIGGVHPPENKLSAGVPIEAAALPKRAFIHLGQHLGAP
                     AKPVVQKGDKVRVGTLIGTGEGFISAAVHSSVSGT"
BASE COUNT          780 a         1375 c         1435 g          826 t
ORIGIN      
        1 tgccgatgag ccgcaggtcg tgggtcaccg cgatgacgct gttgccacgc gcgatgagtc
       61 tcccgaccgc ttcggcgaag agcgccacgt cggcgggatg gagcccggcg gtcggttcgt
      121 cgaagatata gaggtcgttc ccttttccct tcttcgcgag cgcggcggcc agtttcaacc
      181 gctgcgcctc gccgcccgag agggtggtgg cgggctggcc gagggtgata tatcccagac
      241 cgagttcgca cagaatgtcg aggtaccgcg cgagggggcg gtcgtccgcg aagaaacgcg
      301 ccgcgtcgct gatggataga tcgagcactt cggcgatgtt cttcccgttc agcgtcactt
      361 cgcgcacttt ttcactgaac cgcgcgccgt tgcagacatc gcacaccacc tcggaacggc
      421 cgaggaagtg gagccccaac tcgcgcacac cggccccttc gcacgcctcg caacggccac
      481 cggtgaggtt gaaggagaag tggctcgcgg taagcccgcg ctccgtggcg gcggggagcg
      541 ccgcgaaacg cttgcgcagt tcgtcggaga gtttggtgta ggtcgcgggg ttgctgcgag
      601 gattgcggcc gatgggcgac tggtcgatga accggaccgc cgcgacgggc cgctccgcag
      661 aggggagcgc ctccaactgc tcgacgagac attcaacgag cgacgatttc ccggcgccgc
      721 tcaccccggt cacgacggtg aaccccttat gcaggaacga tgcgttgacg gaacgcagat
      781 tattcttcgc cgcgccgcgc acccaaaaat aattcgcgtt gtccggcacc gttgcctccg
      841 gcttggggta gccctctttt tcctgcacca gcgcacgggc cgtggggctc ttttcgcggt
      901 atgcggggtt caggatatcg gcgggggcgc cggcgaagag tatctcgccc cccgcgtcgc
      961 cggggccggg gccgaggtcg atgagatggt cggcgtggcg cgccgcgacg aggttgtggt
     1021 cgaccgcgac gacggtgttc ccggcgtcgc gcagccccgt gaggatgtcc atcagccgcg
     1081 cgtggtcggc cgggtggagc cccgccgagg gttcgtcgag gatgtagagt atcccgcgga
     1141 tccggttctc gagttgcgtg gcgagccgca cccgctgcag ttcgcccccc gaaagcgacg
     1201 ccgcgccgcg cgcaagcggc aggtggtcga tgccgagcgc cgtgaaatgt ctcgcctgtc
     1261 gcgcgacggc ggccgttatc gcgacgagcg cctcgtgccg ttggacggcc gcgaattccg
     1321 cgcaccacgc agcgaattct ttcaccgtca tatcggcgca ctgcgcgatg gagcgtcccg
     1381 cgaccgtcac cgaccgcgcc gtttcattca gccgcgaacc gtgacaggcg gcgcacggga
     1441 ccgaccggac gaagcggagg atgctcgcgt tgcggtcgcg cttcagtatc tcctccatca
     1501 ccgggaggat gccgcggtag taaccctctt cgcgcggttt cgccttgatg ccggtccact
     1561 tcatccggct ctcgagcggg tgtttgccgt acggtatctt tatcttttcc gagccgtaga
     1621 ggacgatccg tttctgttcg tcggtgaggt cccgccacgg gatgtcgacc gaaaaaccgt
     1681 gggcgcggca cacctcgtcg aggaccgcca tggtgacctg cgaatacatg atgtagcccg
     1741 acggcgtcgt gatgacgaag gcgccgccgc gcagcgtttt ggtcgggtcg gcgatgagtt
     1801 tgtcgaggtc gagcgaatct tcgaccccga gcccgccgca gatcgggcag gccccttcgg
     1861 gcgagttgaa cgaaaagagc gaccggttgg gtcgtactcc ctccggtgca ttcccgaagc
     1921 gggcgaacag cagccgcagg aggtcgtaga gccccgagag cgtcccaacg gtcgaacggg
     1981 gcgaagtgac gccgctgcgc tgatcgaccg acacggcggg gggcagacct gtgatctcct
     2041 ttacctcggg gcggtggacc ttgcggaggt actggcgggt ataggtcgag aaggtcccca
     2101 tgaaacggcg catgctctcg tggaatatcg tgtcatagac gagcgatgac ttaccactac
     2161 ccgaaacacc cgtcacgacg gtgaaggcgt tatggggtat ctccacatca atatgcttga
     2221 ggttgtgttc tgcggccccg aagatatgaa gcatgcaccg atctttcgtt atcgatattt
     2281 caccggtatc gacaactctt tcttccccgc cggaacggtg actttcgcgt ccttgaattt
     2341 cggtggcccc atgaccccct tcgcgttgtt cgacacgccg agtccctcct tggggatacc
     2401 gatccagttg gtgtctatct gcccgttccc gttctcatcg tgcatcaccg caacggcata
     2461 gtcgcccggc gcaagtcctt cgaatttggc aaaaagggtg tcgctctccc tgaccggcag
     2521 ggtgatggtc ttgaaggcct tttcaggact cgtcgggaag ccgtcgtcct gattgtacag
     2581 atagacgaac acctgcccct gtttcctgag accccacacc ttgaccgtga gcgtggtggt
     2641 cgcctgcgcc gccgtcaggg cgaaggcaaa gatgatgatc gcgaaaagtg tcgaacgcat
     2701 ggggttcctc cctcggcatc ttaaattacc ggaggtatag ccgttttgtt gacaaagggc
     2761 aatttattta gtagcacggt tccgcattgc gtgcgcatta ccgaacgaat ttacatgagc
     2821 gatacacaca gcgatatctg ccgcgaaggg atcgtgaagg gatacgccga cggtcgcgtc
     2881 cgcgtcctca tcaccgtcca gagcgcctgc gcctcctgcc atgcgaaggg ggcctgcacc
     2941 tcgctcgaca tgtccgaaaa ggtcattgac gcccgcgccg acggcccgtt cgagatagga
     3001 gagaaggtga aggtcgccgt cgcgggtcgc tacggctgga ccgcggtcct ctacgccttc
     3061 gtcctgccga tggtcatcct tttcaccctc ttttttacct gtctttcgac cggaagcggc
     3121 gaaacgcagg cggctctgtc ggggttggcc ggtattgcca tctactttct gctgatccgc
     3181 atcttccgcg accgcctgtt cggccggatg gagttccatg ttgaacgcat caccaactgt
     3241 caatagaggt gtcctgtgag tgccatgatc ctgtacccgg tcatcatgct cggcgcggtc
     3301 gccggtatcg cggcgatcct gctgttcctg acggccaaga agttccatgt cgaagaggat
     3361 ccgcgcatcg acaaggtggc cgaactgctt cccggcgcca attgcggcgg ctgcggctac
     3421 cccggctgtc gcggccttgc tgaggcgatc gtcaaggcgg ccgacaaggg cgatatcggc
     3481 gggctttcct gccctcccgg cggctcggcg gcgatgaagg cgatcggcga ggtgctcggg
     3541 atggcggtgg ccgagagcga gccgatggtg gccgtgctcc gctgcggcgg cacccacgaa
     3601 aaggcgcccc gcacactgaa gtacgacggc gctcccaagt gcgtcatcgc tcataccgtc
     3661 tttgccggcg aaaaaggctg tccgaacggc tgcctcggac tcggcgactg cgtgacggtc
     3721 tgcgcgttcg gcgcgatgta tatggacgaa aagaccgggc tcccggtcat agatccagcg
     3781 aaatgcgtcg cctgcggcgc ctgtgtcaag gcctgtcccc gcaagatcat cgagatgcgt
     3841 cccaagggac ggaaggatcg ccgcgtctgg gtgaactgcg tgaataccga aaagggcgcc
     3901 gccgcgatga agtactgcaa ggcggcctgc atcgggtgcg gcaagtgcgt gaaggaatgc
     3961 cccgagaagg tgcaggcgat aacgctccag aacaacctcg cctacatcga cccgaagaag
     4021 tgcatcgcct gcggcaagtg cgtgctcgtc tgccccacca aggcgatact cgccaccttc
     4081 gaaccgccca agccgaaacc ggtcgagtct cagtcggccg aagcgcccaa ggcgacggtc
     4141 tgaccgatgg aacaacaacg ggacaaagga gatatgtcgt gaatctcaag accttcagga
     4201 tcggcggcgt tcatccgccg gagaacaaac tgtcggcggg ggtccccatc gaggcggccg
     4261 ccctgccgaa acgggccttc atccatctcg gccagcacct cggcgcaccg gcgaaaccgg
     4321 tggtccagaa gggcgacaag gtgcgggtgg ggacgctcat cgggacgggc gagggcttca
     4381 tctcggccgc cgtccattcg tcggtgtcgg gcacca
//
LOCUS       DAOQUE010000224         4415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_22073_length_4415_cov_5.709174, whole genome shotgun sequence.
ACCESSION   DAOQUE010000224 DAOQUE010000000
VERSION     DAOQUE010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22073_length_4415_cov_5.709174"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3067)
                     /locus_tag="PKH10_07690"
     CDS             complement(<1..3067)
                     /locus_tag="PKH10_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28045.1"
                     /translation="MKGGGYECFRVVAPPLIVLFLFFLLSLSLEPLQASTTGAGGSGK
                     PLLVKMRKDLFEKIILNKLNENLGTIPLDSQGLVTAKLVATDVLLGRETFSRIKEFYV
                     AEDILFPSEINDEPLNEKNQMLIFIKFSDISSGSLEIDRYYNDFFKTFSYSPMYGSLN
                     YDENAPTMFVTAALMLMPDTNDPSKKIWRTILLHLQITDLLDMLAPMTNLESVIFSSL
                     ANKDIPCVDLISMNQSFPVQGLTIESGNAELAISPPSSFALESQGIAANVTVGTEPIA
                     YDSAPILPAGSYEEIQQAGTLEEIHFSNDIFTRIFSEKLNNAVNDSGAPIRIEDGREG
                     VFFKFYRNENTPSGGYLGFDLHVDGTLCIASFDILFNISKDSDGSLKMVKSDCVWQIN
                     NLGCEILYDIIAGFIPGSTDACDLIDSVANSSLTIDISSLATTFINKVVNEGYERVIY
                     TYRVEIVPGFNFFSDVVNDLVLDIQGQNPFSPNYASAPYYARWNWMFADNDQDGWINI
                     DDVCPFSRDNAICDRDGDFLRDDVDEKVYYTVADLYPSKRDETPANKQCSLASGEWGF
                     LRLCHYDVTVHHNETIKIDSSIGLKETWNGDPTDQTYLSSYHCYCGSDDLSRQDCNPS
                     GACGLNHAKPNEIRNGDYSNQLSWQPVNRAPNRLYTDTPCEGSGSILPYRKRNAITGT
                     CAADSSVAWEWQEDLAIIYNVTSIAEWDDVNRLSLYGGRTQKEYFTEVDPIFHAARFS
                     HGPARTFEQSAYTVGFGSDEKINPSYFENSRVTGDEVIDYNILNSNFMPNWASSKAEM
                     VYLMKWFTQEPRNTVAGKFNHWHYPHELMHDLIYPGLLDPSGPVSEPGFLTPDYFYRA
                     SLVADHLQLSKNPVQPGRQAIVATASGPAVYYWKESSHFLSPAHSGAYETVGKPIRNG
                     VDMRYASSAQKSGVLYAAGSLVRSMEPSIDDDPFASYNFAPIFGKIVDEGNNFNYISL
                     ASIPANGIRTLRLGTMNMTLYVLTYGSNNHLAVFSYDENGDVWNMRSTTI"
     gene            complement(3159..>4415)
                     /locus_tag="PKH10_07695"
     CDS             complement(3159..>4415)
                     /locus_tag="PKH10_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28046.1"
                     /translation="DEPSIGLHAADTEKLISILRNLRDQGNNVIVVEHDRDIMEASDI
                     IVDLGPGAGRKGGELLYAGPAKGIGAVKRSLTGRYLSGELAIPAPKKRRKPSGWIRLT
                     GARTNNLKNIDVAVPLGVLCAVTGVSGSGKSSLIMETLLPMLEGKPAPAKRFSVTGTL
                     DEFLEIDQSPIGQTPRSNPATYTQAFGPIRDLFAQLPESRARGYLPGRFSFNVKGGRC
                     ERCEGAGLIRIEMNFMPDTYVTCPDCGGKRFNPDTLEIKYKGKSIYDVLEMEVDEAVD
                     LFQPFPAIRRKLELLHNIGLGYIALGQPAHTLSGGEAQRVKLSKELAKKKADRTLYIL
                     DEPTTGLHFDDIRKLLVILNRLVDRGASVLIIEHNPDIIKCADHIIDLGPQGGGKGGE
                     IVACGTPEEVARSARSLTGKMLKNIL"
BASE COUNT         1054 a         1092 c         1192 g         1077 t
ORIGIN      
        1 gaatcgtcgt actccgcata ttccatacat cgccgttctc gtcataggaa aaaaccgcta
       61 aatggttgtt ggaaccataa gtcagcacat agagcgtcat attcatcgtg ccaagtcgga
      121 gggtgcggat tccgttagcc ggaatggatg caagtgatat atagttgaaa ttgttcccct
      181 cgtctacaat cttgccaaaa atcggcgcaa agttgtagct cgcgaatgga tcgtcgtcaa
      241 ttgacggttc cattgaacgc acaagcgatc cggcggcata caaaacaccc gatttttgcg
      301 cggacgaggc atacctcata tccacgccgt tgcggatcgg tttacctacc gtttcgtatg
      361 cgccgctatg tgctggcgaa aggaaatgag aactttcctt ccagtaatat accgccggac
      421 cgctcgcagt cgcaacgatt gcctgacgcc ccggttgcac cggatttttg ctcaattgga
      481 gatgatcggc tacaagggaa gcgcgataaa aataatcggg cgttaaaaac cccggctcgg
      541 aaacaggacc gctggggtcg agaagacccg gatagatcaa gtcatgcatg agttcatgcg
      601 gataatgcca atgattgaat ttccccgcca cggtatttct cggttcttgc gtgaaccatt
      661 tcatgagata taccatctcc gcttttgacg aagcccagtt gggcataaaa ttgctattta
      721 aaatattata atctatgacc tcatcgccgg taacccgcga gttctcgaag taggatgggt
      781 tgatcttttc gtcgcttccg aaacccacgg tatatgccga ttgttcgaaa gttcgtgcgg
      841 ggccatggga gaagcgggcg gcgtgaaaga tgggatcaac ttcggtaaaa tactccttct
      901 gggttcgccc gccgtaaaga gagagccggt tcacatcgtc ccattcggca atggatgtga
      961 cattgtatat aatggcgaga tcttcctgcc attcccacgc gacagagcta tctgccgcgc
     1021 aggttcccgt aattgcattg cgcttgcgat agggtaagat agaaccggaa ccctcgcagg
     1081 gggtgtcagt gtagagacgg ttcggagccc ggttgaccgg ttgccaagag agttggttgc
     1141 tataatcccc gtttcttatc tcgttcggtt ttgcgtgatt aagtccgcag gccccgctgg
     1201 gattacaatc ttgtcgggaa agatcgtcgc ttccacagta acagtggtaa ctcgagaggt
     1261 aggtctggtc ggtcggatcg ccgttccatg tttctttcag accgatggag gaatcaattt
     1321 tgatcgtttc gttatgatga acggtgacat cgtaatggca aaggcgaagg aaaccccact
     1381 ctccactagc gagggagcat tgtttgttgg cgggggtctc atcccgtttc gatggataaa
     1441 gatcggcgac ggtgtaatag accttttcgt cgacatcgtc gcgcaaaaaa tcgccgtcac
     1501 gatcacagat ggcgttgtct cgggaaaagg gacaaacatc atcaatgttt atccagccgt
     1561 cttgatcgtt atctgcgaac atccaattcc agcgagcata atacggagcc gaagcgtagt
     1621 tgggggaaaa gggattttgc ccctgtatgt cgagaacaag atcattcacg acatccgaaa
     1681 agaaattaaa tccgggtaca atttcgacac gataagtgta tatgactcgt tcgtatcctt
     1741 cattaacaac tttattgata aaagttgttg ccaatgagga gatgtctatg gtaagggaac
     1801 tgtttgcgac agagtcgata agatcgcacg catcggtgct acccgggatg aatcccgcaa
     1861 tgatatcgta aagaatttcg caccccaagt tgttgatttg ccagacgcag tcacttttga
     1921 ccattttaag ggagccatca gaatctttac ttatgttgaa taggatgtcg aacgaagcga
     1981 tgcataatgt gccatccaca tgcaaatcaa atcccagata accgccagaa ggcgtattct
     2041 cgttccgata gaacttaaaa aaaacgccct cacgaccatc ttctatgcgt ataggagctc
     2101 cgctgtcatt gacagcatta ttcaattttt cgctgaatat tcgcgtaaaa atatcattcg
     2161 agaaatgtat ttcttcaagc gtaccggctt gttgaatttc ttcatagctt ccagcaggca
     2221 aaatgggagc agaatcataa gcaataggtt cggttcccac cgtaacatta gcggcgatgc
     2281 cttgcgattc gagcgcaaaa gaagaggggg gggaaatcgc caactcggcg tttccgcttt
     2341 ctattgtcaa cccttgaacg gggaaagatt gattcatcga gatcaaatca acacagggga
     2401 tatctttgtt cgccaaagaa gaaaaaataa cgctttctaa gttggtcata ggtgctagca
     2461 tatcaagcaa gtcagttatc tgaagatgca agagtatagt gcgccatatt ttcttgcttg
     2521 gatcgttggt gtccggcata agcataagtg ccgctgtaac gaacatggtg ggggcatttt
     2581 cgtcataatt aagggaccca tacatagggg aataagaaaa agttttgaaa aagtcattgt
     2641 aatatcgatc aatctccaac gaaccggaag atatatcaga aaactttatg aaaataagca
     2701 tttgattttt ttcgtttaag ggttcgtcgt tgatttctga ggggaaaagg atatcttcgg
     2761 caacataaaa ctctttaatt cgactaaagg tttctcgtcc cagaagaaca tcggtcgcga
     2821 cgagttttgc ggtgacgagt ccttggctat caagcgggat agtcccgagg ttttcgttga
     2881 gtttgttaag tattatcttc tcgaaaagat cttttctcat ctttacgagg agcggttttc
     2941 ccgaaccacc cgctccagtc gtcgatgctt gtaagggctc aagagaaaga gagagcagaa
     3001 agaagagaaa aagaacgatt aggggggggg caaccacacg gaaacactca taacccccgc
     3061 ctttcataat gacctccctt gaaagtgatg atatcccacg caaaaagaat ctaccatgtg
     3121 ggaggatgag agtcaagagg gaaagagagg gatttttttc acaggatatt cttcagcatc
     3181 ttgccggtga gcgaccgggc gctccttgcc acctcttccg gcgtgccgca ggcgacgatc
     3241 tcgccgccct tgccgccgcc ctgcgggccg agatcgatga tatggtcggc gcacttgatg
     3301 atgtcggggt tatgctcgat gatgaggacc gacgcccccc ggtcgacgag ccggttcagg
     3361 atgacgagca gtttcctgat gtcgtcgaaa tggagccccg tcgtcggctc gtcgaggatg
     3421 tagagcgtcc ggtcggcctt cttctttgcg agttccttcg acagtttcac gcgctgcgcc
     3481 tcgccgcccg acagcgtgtg ggccggctgg ccgagcgcga tgtagcccag cccgatgttg
     3541 tggaggagtt ccagtttgcg ccgtatcgcc ggaaagggct ggaacaggtc gaccgcctcg
     3601 tccacctcca tctcaagcac atcgtagatg ctttttccct tgtactttat ctcgagcgtg
     3661 tcgggattga accgcttgcc gccgcagtcg gggcaggtga cataggtgtc gggcatgaag
     3721 ttcatctcga tgcggatgag tcccgccccc tcgcaccgct cgcaccgacc gcccttgaca
     3781 ttgaacgaga accggcccgg caggtagccg cgggcccgcg attcgggcag ttgggcgaac
     3841 aggtcgcgta tcgggccgaa cgcctgcgtg taggtcgccg ggttactgcg cggtgtctgg
     3901 ccgatgggcg actggtctat ctcgaggaac tcgtcgagtg tgcctgtcac cgagaatctc
     3961 ttcgccggcg ccggtttccc ttcgagcatc gggagaagcg tctccatgat gagcgacgat
     4021 ttgcccgaac ccgacacgcc ggtcaccgcg cacagcaccc cgagcgggac ggcgacatct
     4081 atgttcttga ggttgttggt ccgcgcgccg gtcagccgta tccagccgga gggcttgcgc
     4141 cgctttttcg gcgccggtat cgccagttcg ccggaaaggt atctgccggt gagcgatcgt
     4201 ttcaccgcgc cgatgccctt cgccggtccg gcatagagca gttcgcctcc cttgcgtccc
     4261 gcaccgggac cgagatcgac gatgatgtcg gaggcctcca tgatgtcgcg gtcgtgttcg
     4321 acgacgatga cattgttccc ctgatcgcgc aggttgcgca ggatcgatat cagtttctcg
     4381 gtgtcggcgg cgtgcaggcc gatggagggc tcgtc
//
LOCUS       DAOQUE010000225         4412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_22094_length_4412_cov_3.837044, whole genome shotgun sequence.
ACCESSION   DAOQUE010000225 DAOQUE010000000
VERSION     DAOQUE010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22094_length_4412_cov_3.837044"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PKH10_07700"
     CDS             <1..1184
                     /locus_tag="PKH10_07700"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNT28047.1"
                     /translation="REKYPVSYGTTLFVKDGDRVKEGQVLAEWDPYNTPIIAETEGFV
                     KYDDIDNGVTVREELDAQTGLSKIVVINSRDPKKLPKIMITDAAKKKAKKIDRGEALY
                     HLPVNAYIEVKDGEEITAGKIVAKVPREARKTKDITGGLPRISDLFEARRPKTPAIVS
                     EIDGYITISTETKGAKNKTMITVTPEQGEQRAYLVPKEKHINVHDGDFVEAGESLIDG
                     SIDPHDVLRIKAETEVAKFLVQEVQKVYKLQGVPINDKHIEVIVRQMLRKVRIVDPRD
                     TAFMKGELIEKEILEAANLKVENEGKEPARWETALLGITKASLNTESFISAASFQETT
                     KVLTNASVERKVDTLRGLKENIIIGKRIPAGTGFPKYHEAHYEVVVLDQANETPDEQE
                     R"
     gene            1373..1744
                     /gene="rpsL"
                     /locus_tag="PKH10_07705"
     CDS             1373..1744
                     /gene="rpsL"
                     /locus_tag="PKH10_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNT28048.1"
                     /translation="MPTINQLIRKGREAVRYKSKCPALVACPQRKGVCVRVYTTTPKK
                     PNSALRKVARVRLTSGFEVTCYIPGEGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTH
                     DTAGVDGRRQSRSKYGAKRPK"
     gene            1759..2229
                     /gene="rpsG"
                     /locus_tag="PKH10_07710"
     CDS             1759..2229
                     /gene="rpsG"
                     /locus_tag="PKH10_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNT28049.1"
                     /translation="MPRKAFISRREILPDAKFGEVLIQKFINKVMWDGEKSVAEKIVY
                     SSLDKAAKGKDGGPVEVFRRALENVRPKVMVKSTRMGGANYQVPTEVTLEKSQSIGMK
                     WIITAARGRSEKGMVERLAAEFDDAYNKRGNAYKKMDETHKMAEANKAFAHFKW"
     gene            2251..4335
                     /gene="fusA"
                     /locus_tag="PKH10_07715"
     CDS             2251..4335
                     /gene="fusA"
                     /locus_tag="PKH10_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNT28050.1"
                     /translation="MAVKEKNIALSDIRNIGIMAHIDAGKTTTTERILFYTGVSYKMG
                     EVHEGTAVMDWMDQEQERGITITSAATTCYWKDHRVNIIDTPGHVDFTIEVERSLRVL
                     DGAIAVFCAVAGVQPQSETVWRQADRYHVPRIAFVNKMDRVGADLDNVISMMRERLGA
                     NAVAIQEPIGSEDKFVGVVDVIDRIAYYYDNDMLGAQYRIEKEIPSHLIERVEKARER
                     LVEAISEFDEDLMRSYLDGRDIDAATIRKVLRKGVLSSSIFPVLCGSAFKNKGIQLLL
                     DGVIDYLPAPDDLPPVKGVHPRTGKDVFRRPEDKELGAYVFKIMSDPYVGELNYIRVY
                     SGEIQNGQLVYNATVDQQERIGRLLRMHANKREDVDIIRAGNIGAVVGLKYGATGSTL
                     CDKAHQIVFEPMTFPEPVISIAIEPKTKADEEKLGYSLQKLSKEDPSFKVSTNAETAQ
                     TIISGMGELHLEVLVERLRREFKVEANVGKPQVSYRESVPRPVRHEYKQVKQTGGRGQ
                     YAHVVLNLEPLPRGGGLQFEEKIKGGVIPQEYFNSVKKGVEAAATSGRYGFPVIDLRV
                     TLVDGSAHEVDSSDIAFRIAASACFREAMERSDSCILEPIMAVEIIIPEEYLGDVLAD
                     LRSRRGEVEGMSDGRGSRVKMVRAQVPLSEMFGYSTDLRSCSQGRGTYTMQFAFYREI
                     PEQIKTKLFPYY"
BASE COUNT         1108 a         1151 c         1334 g          819 t
ORIGIN      
        1 aacgagagaa atacccggtc agttacggca cgaccctgtt cgtcaaggat ggtgatcgcg
       61 taaaggaggg acaggtcctc gccgagtggg atccctacaa tacgccgata atcgccgaga
      121 ccgaaggttt cgtcaaatac gacgacatag ataacggtgt cacggttcgc gaagaactcg
      181 atgcgcagac cggtctttcg aagatagtcg tcataaactc gcgcgacccc aagaaactgc
      241 cgaagatcat gataaccgat gccgcgaaga aaaaggcgaa gaagatagat cgtggcgagg
      301 ctttgtacca cctcccggtc aacgcctaca tcgaggtgaa ggacggcgaa gagatcaccg
      361 ccggtaagat cgtcgccaag gtgccgcgcg aggcccgcaa gaccaaggac atcaccggcg
      421 gtctgccgcg catatccgat cttttcgagg cgcgtcggcc caagacgccg gccatcgtca
      481 gcgagataga cggctatatc accatatcga ccgagaccaa aggagccaag aacaagacga
      541 tgataacggt cacgcccgag cagggtgaac agcgcgccta tctggttccc aaggaaaaac
      601 acatcaatgt gcatgacggc gatttcgtgg aggccggcga atcgctcatc gatggttcga
      661 tagacccgca cgacgtcctg cgcataaagg cggagaccga ggtcgcgaag ttcctcgtgc
      721 aggaggtcca gaaggtctat aaacttcagg gcgtcccgat aaacgacaag cacatcgagg
      781 tcatcgtgcg tcagatgctc cgcaaggttc gtatcgttga tcctcgtgac accgccttca
      841 tgaaaggcga actcatcgag aaggagatac tggaggcggc gaacctgaag gtggagaacg
      901 aaggcaagga accggcgcgt tgggagaccg cgctccttgg catcaccaag gcctctctta
      961 atacggagag cttcatatcg gccgcatcgt tccaggaaac gaccaaggtc ctgaccaacg
     1021 ccagcgtcga gcgcaaggtc gatacgctac gcggactcaa ggagaacatc atcatcggta
     1081 agcggatccc cgccggtacc ggtttcccca agtaccatga ggcgcattac gaggtggtcg
     1141 tgctggacca agcgaacgag acccccgatg aacaggaacg ataacgcaaa ggaacagttg
     1201 gcttaaaaga gctcttttct ctttggcggc gctttagagc gccgcttttt ttttcttgta
     1261 gttatagtaa gttacctttg ctcgggaaaa aatctttgac aggtgaggac ccgacactac
     1321 aatcgccacc gttttttgcg caacctaaca ggagagacag gggagaacaa ggatgccgac
     1381 gatcaaccag ctgatcagaa aaggaagaga agcggtccgt tacaagagca aatgtccggc
     1441 gttggtggcg tgtccccaac ggaagggagt gtgcgtgcgc gtctacacga cgacgcccaa
     1501 aaaacccaac tcggcccttc gcaaggtggc tcgtgtgcgc ctgacgagcg gcttcgaggt
     1561 gacctgctac atccccggtg aggggcataa cctgcaggaa cacagcgtgg tcctcgtgag
     1621 aggcggtcgc gtcaaggacc ttcccggcgt tcgttatcac atcgtcagag ggacccacga
     1681 taccgccggg gtcgacggtc gtcgtcagag ccgttccaag tacggcgcta agagacctaa
     1741 gtaacggagg gatagatcat gccgagaaaa gcgttcatat cgcgcagaga gatacttccc
     1801 gacgccaagt tcggagaggt cctcatccag aaattcatca acaaggtgat gtgggatggc
     1861 gagaaatcgg tcgccgagaa aatagtatat tcctcccttg ataaagcggc caaaggtaag
     1921 gacggcggcc ctgttgaggt gttccgccgc gcgcttgaga atgtgcggcc caaggtcatg
     1981 gtgaaatcga cccgcatggg tggtgccaac tatcaggtac cgacggaagt tacgctcgag
     2041 aaaagccaat ccatcgggat gaagtggatc atcaccgcgg cgcgcggacg ttccgagaag
     2101 ggtatggtcg aacggcttgc ggccgaattt gatgatgcct acaacaagcg tggcaatgct
     2161 tacaagaaga tggacgagac gcataagatg gccgaggcta acaaggcctt cgctcacttc
     2221 aagtggtaac gacgggacag atatagaccc atggccgtga aggaaaagaa catagcgctt
     2281 tcagatatcc ggaacatcgg tatcatggcg catatcgacg ccggcaagac cacgacgacc
     2341 gagcgcattc tcttctacac gggcgtttcc tacaagatgg gcgaggttca cgaaggcacg
     2401 gcggtcatgg attggatgga tcaagaacag gagcgcggta tcacgataac aagcgccgcg
     2461 accacctgtt actggaagga ccatcgcgtc aatatcatcg ataccccggg ccatgtcgat
     2521 ttcactatag aggtcgagcg gtcgctgagg gtccttgacg gtgccatcgc ggtcttctgc
     2581 gcggtcgccg gcgttcagcc gcagtcggaa acggtctggc gtcaggccga ccggtaccat
     2641 gtgccgcgta tcgcgttcgt caacaagatg gatcgcgtcg gggccgacct cgacaacgtc
     2701 atctccatga tgcgggaacg cctcggcgct aacgcggtgg cgatacagga accgatcggg
     2761 tctgaggaca agttcgtcgg cgtcgtcgat gtcatcgacc gtatcgctta ctactacgac
     2821 aacgacatgc tgggcgcgca atatcgcatc gagaaagaga taccttcgca ccttatagag
     2881 cgcgtggaaa aagcccgcga aagattggtc gaagcgatca gtgaatttga cgaagacctg
     2941 atgaggtcgt atctcgatgg tagagatatc gacgcagcga ccatcaggaa agtgttgcgc
     3001 aaaggggtgc tttcctcatc gatattccct gtcctgtgcg gttcggcatt caagaacaaa
     3061 ggcattcagc ttctgcttga tggcgtcatc gattatttgc cggcgccgga tgatcttccc
     3121 ccggtcaaag gagtacaccc gagaacgggc aaagatgtgt ttcgccgccc agaggacaag
     3181 gagttgggcg cctatgtgtt caagataatg agcgacccct atgtgggaga gctcaactat
     3241 atccgtgtct attccggaga gatacagaac gggcaactgg tctataacgc gacggtcgat
     3301 cagcaggaac ggatcggtcg ccttctgcgc atgcacgcga acaaacgaga ggatgtcgac
     3361 atcatccggg cggggaacat cggcgcggta gtgggactca aatacggggc aaccggttcg
     3421 acgctctgcg acaaagcgca ccagatagtg ttcgaaccga tgaccttccc ggaaccggtc
     3481 atctcgatcg cgatagagcc gaagaccaag gcggacgagg agaaacttgg ctattctctg
     3541 cagaagctct cgaaagagga tcccagtttc aaggtgagca ccaacgcgga aacggcgcag
     3601 accatcatat cgggaatggg cgagctgcat ctggaggtcc ttgtcgagcg gctccgccgg
     3661 gaattcaagg tggaggccaa tgtcggcaag ccgcaggtga gctatcggga gtccgtcccg
     3721 agaccggtca ggcatgagta caaacaggtt aagcagaccg gtggcagagg gcaatacgcg
     3781 catgtcgttt tgaatttaga gcccttgccg cgcggtggcg gccttcaatt cgaggaaaag
     3841 ataaagggcg gcgtcatccc gcaggaatat ttcaattccg tgaagaaggg ggtcgaagcc
     3901 gccgctacaa gcgggagata cggtttcccc gtcatcgacc tgagagtgac gctggtcgat
     3961 gggtcggccc acgaggtcga ttcttcagac atcgcgttcc gtatcgcggc ctcggcgtgt
     4021 ttccgagagg cgatggaacg gagcgactcc tgcatactcg aaccgatcat ggcggtcgag
     4081 atcatcatcc ccgaggagta tctgggggat gtgctcgccg atctgcgctc gcgccgtggc
     4141 gaggtcgaag gtatgagcga cggtcgtgga tcccgggtca agatggtccg tgcacaggtc
     4201 ccgctgtcgg agatgttcgg ttactcgacc gatctgcgtt cctgttcgca ggggagaggg
     4261 acctacacga tgcagttcgc tttttatcgc gagatacccg agcagatcaa gacaaaactg
     4321 ttcccatatt attaactgaa cgaggaagga ggaggagaga tggcgaagga gaaattcaac
     4381 aggacgaagc cgcatgtgaa tgtggggacg at
//
LOCUS       DAOQUE010000226         4404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_22147_length_4404_cov_3.650724, whole genome shotgun sequence.
ACCESSION   DAOQUE010000226 DAOQUE010000000
VERSION     DAOQUE010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22147_length_4404_cov_3.650724"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..1143
                     /locus_tag="PKH10_07720"
     CDS             31..1143
                     /locus_tag="PKH10_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28051.1"
                     /translation="MKVPMSMIAALCMASLLAVACSNSAKNVTPDNGGVGDGDTLSTD
                     AENALPDTGVDAGPDIGPDADPDAVIGEDDDLFAGDDIGADDIAETDDIPSDADSGAA
                     DDGTTPDDDALLSDETTPDADADIDTDTVEPIIGRYLVSIDNRTDATRLLKVDIDTGE
                     GTEVCVLPAPYNGYNYNSLTFNRAGYLFAHCRGVSGEVSQIHRIDPCTCEVTVLGDSG
                     FSSIPGITADRVNGLFGIETTQDLFLTIDTATGAGTSIGPLGVDFGTSGATWSDEDDT
                     VYGINGTDDSLYVIDPDDGVATFLIAITGVDLGTVGVELNPFDGVIYACTDDGILYAV
                     DPITGIATAIGTGMGHQTSCSNLGAPWTEVTCLEEL"
     gene            complement(1156..2016)
                     /locus_tag="PKH10_07725"
     CDS             complement(1156..2016)
                     /locus_tag="PKH10_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNT28052.1"
                     /translation="MKRYIAGIDLGGTKIYTAVASKNGAILAEVKLPTGATRSKMAIF
                     ADIVRSVEMACEKAGIATKDIAAIGIGIPGPIDHATGTVRVCPNIPSWKKVPVRDLLA
                     ERFGCPVHVENDARVAGLAEALRGEGIGLSRVFYTTVSTGIGGAIILDGKIYHGATGV
                     AGEIGQTRLPDGTIFEQAAAGPAIKRLFGIDPEDIPALLRTGDKRAQAALEHLTRLIG
                     LWLANVSTLLNPDLIVIGGGLANLGPCFFTPVRRHIRDNAFSESAKVKIVRAALGERS
                     GVIGALELCR"
     gene            2160..2549
                     /gene="secG"
                     /locus_tag="PKH10_07730"
     CDS             2160..2549
                     /gene="secG"
                     /locus_tag="PKH10_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNT28053.1"
                     /translation="MFTVFAVLQVIISVLMITVILLQEGKKGMGAIFGGSSSSIFGAR
                     GAANILAKATTVLAILFFINSVWLAHLSSGQKSVVDKVAPEMTSTKTEEPAPVPAPSP
                     VEAPAPAPAEAPAPAPTPAPAPAPAQQ"
     gene            2599..2683
                     /locus_tag="PKH10_07735"
     tRNA            2599..2683
                     /locus_tag="PKH10_07735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2633..2635,aa:Leu,seq:aag)
     gene            complement(2702..3397)
                     /locus_tag="PKH10_07740"
     CDS             complement(2702..3397)
                     /locus_tag="PKH10_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT28054.1"
                     /translation="MTMTIAQVFDVVRAVKRPLCVFDIDSTLMNTGSRSHRILREAAQ
                     EAFPELAPFADKLCSCNMAYHLTDDLRRLGFDNEKELAAVDRFWAARFFTDAYVLYDL
                     PYPGAVDYVRDLHREGVFIYYLSGRDEPNMGHGTRESFQRHGFPLDDRTTMHLKPDFA
                     MNDLLFKRDAFADIQAMGHTTVAIFENEPANANAFKEHFPGAAVFLIRTITSNNPAPL
                     RTDVIPFDSFEDR"
     gene            complement(3397..>4404)
                     /locus_tag="PKH10_07745"
     CDS             complement(3397..>4404)
                     /locus_tag="PKH10_07745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT28055.1"
                     /translation="LRTVYIVLYAVNMYFQSYGAVSIVKVNANWFGLTERGTFSGIFG
                     IMIASGIYLAFDVTGSIIASPLVAGQGFNGGDALWWVFLSPAVLLLFIFAVEWFLLRD
                     RPAQAGFSDFETGDASAGEDLDERLSPLQLYKRILTHPVILTIAAIEFCTGVLRNGVM
                     HWFPIYAKEQITAGVTAGFGFMLDHWGLLLMVAGIIGGNVAGLVSDKLFQSRRAPAAL
                     FLYGALVLCTAAMLASLANGWLLGALVFFMSVAVIGTHGLLSGTATMDFGGRKGAATA
                     VGVIDGFVYLGTGFQSLSLGYITTRDWSFWPVFLLPFGVAGMLLLLRIWNAKPGRAGG
                     H"
BASE COUNT          868 a         1366 c         1369 g          801 t
ORIGIN      
        1 catcgatctg gtaaagaggg aggaggcatc atgaaagttc cgatgtcgat gatcgcggcg
       61 ctgtgcatgg catcgctctt ggctgtcgct tgctccaata gtgcgaaaaa tgtcacgccg
      121 gacaacggtg gtgtcggcga tggcgacacc ctgtcgaccg atgccgagaa cgcgttgcct
      181 gacaccggtg tcgacgcggg tcccgatatc ggcccggacg ccgaccccga cgccgtgata
      241 ggggaggatg acgatctttt cgccggcgac gacatcggcg ccgacgatat cgcggagacc
      301 gatgatatcc cctccgacgc cgacagcggc gcggcagatg acgggacgac gcctgatgac
      361 gacgcgcttt tgagcgatga aacgaccccc gacgccgatg ccgacattga cacggacacg
      421 gtagagccga tcatcggtcg ctatcttgtg agcatcgaca atcgcaccga tgcgacgcgt
      481 ctgctcaagg tcgatatcga caccggggag gggaccgagg tctgtgtcct gccggcgccc
      541 tataacggct ataactacaa ctcactcacc ttcaaccgcg ccggctatct cttcgcccac
      601 tgccgcggcg tgagcggcga ggtctcgcag atccaccgca tcgacccatg cacctgcgag
      661 gtgacggtac tcggcgattc tgggttctcc tcgatccccg gcatcacagc tgaccgcgtc
      721 aacggattgt tcggtatcga gacgacgcag gacctgttcc tcaccatcga taccgcgacc
      781 ggcgccggga cgagcatcgg cccgttgggg gtcgatttcg gcacatcggg cgccacttgg
      841 tccgacgagg acgacaccgt ctacggtatc aacggtaccg acgatagttt gtatgtcatc
      901 gatcccgacg acggagtggc gaccttcctt attgccatca cgggggtcga tctcgggacg
      961 gtgggggtgg aactgaaccc gttcgacggg gtgatatacg cctgcaccga tgacggtatc
     1021 ctctacgccg tcgacccgat caccggcatc gcgaccgcca tagggaccgg catgggacac
     1081 cagacctcct gttccaatct cggtgcgccg tggaccgagg tcacctgtct ggaagaactc
     1141 tgaccgatcg ggccttcacc ggcacagttc cagcgcgccg ataacccccg atcgctcacc
     1201 caacgccgcc ctgacgatct ttaccttcgc gctttcgctg aaggcattat ccctgatatg
     1261 ccgcctgacc ggtgtgaaaa agcaggggcc gaggttcgcg agtccgccgc cgatgacgat
     1321 gagatcgggg ttgaggagcg tcgagacatt ggccagccat agaccgatga gacgggtgag
     1381 gtgctcgagc gccgcctgcg cccgcttgtc acccgttcgg agaagcgccg ggatatcctc
     1441 ggggtcgatg ccgaagagcc gtttgatggc gggaccggca gccgcctgtt cgaagatggt
     1501 cccgtcgggg agccgtgtct ggccgatctc gcccgcgacc cctgtcgcac cgtgatatat
     1561 cttcccgtcg aggatgatgg cgccgccgat gccggtcgag acggtggtgt agaatacccg
     1621 tgaaagaccg atcccctcgc cgcgcagtgc ctcggcgagc cccgcgaccc gcgcgtcgtt
     1681 ctcgacatgg accgggcacc cgaaccgttc agcgagcagg tcgcgcaccg gtaccttctt
     1741 ccacgagggg atgttggggc acacccgcac cgtgccggtc gcgtggtcga tggggccggg
     1801 gatgccgatg ccgatggcgg caatatcctt cgtggcgatg cccgcctttt cgcaggccat
     1861 ctcgaccgac cggacgatgt cggcgaatat cgccattttc gaccgtgtcg cgccggtcgg
     1921 cagtttcacc tcggcgagga tggcgccgtt cttcgaagcg acggcggtgt atatcttcgt
     1981 gccgccgagg tcgatgccgg cgatatatcg cttcatgggg cgctccgttg gtggatacat
     2041 cgccgcactg tatcagaacc gcgccgcttt ttccagagcg tccggagaaa aatgttgaca
     2101 acacccgaaa aaacgctata catcgccggt gttttatgaa cctcccgcag ggagccagaa
     2161 tgttcacggt atttgcggtg ttgcaggtga tcatatcggt cctcatgatc accgttatcc
     2221 tgttgcagga aggtaagaag ggcatgggcg cgatattcgg cggctcgtcg agttccatct
     2281 tcggcgcccg cggcgcggcg aacatcctcg ccaaggcgac cacggtgctg gccatcctct
     2341 tcttcatcaa ctcggtctgg ctggcccatc tgtcgagcgg tcagaagtcg gtcgtcgaca
     2401 aggtcgcgcc cgagatgacc tcgacgaaga ccgaggagcc cgctccggtc ccggctccct
     2461 ctccggtgga agccccggct ccggctccgg cggaagcgcc cgctcccgct ccgaccccgg
     2521 ctccggctcc ggctccggca caacagtaag cgttaacggt ccccggttct tttacaggtg
     2581 atcatatcac cgaaaggtgc agaagtggtg gaattggcag acacgcaaga ctaaggatct
     2641 tgtgccgcaa ggtatggggg ttcgactccc cccttctgca ccatcttttt tttctaaagg
     2701 atcagcgatc ttcaaaagag tcgaacggaa tgacatcggt gcgcaggggc gctggattgt
     2761 tcgatgttat cgtccgtatc aggaacaccg ccgccccggg aaaatgctct ttgaacgcgt
     2821 tcgcattggc cggctcgttc tcgaatatcg ccaccgtcgt gtggcccatc gcctgtatgt
     2881 cggcgaaagc gtcgcgcttg aatagcaggt cgttcatcgc gaagtctggc ttgagatgca
     2941 tcgtcgtccg gtcgtcgagc gggaaaccgt gacgctggaa cgactcgcgg gtgccatgtc
     3001 ccatgttcgg ttcgtcgcgt cccgacagat agtaaatgaa taccccctcc cggtgcaggt
     3061 cacggacata gtccaccgcg ccggggtagg ggagatcgta caggacatag gcgtcggtga
     3121 agaagcgggc ggcccagaac cggtcaaccg ccgccagttc cttctcgttg tcgaagccga
     3181 gccgtcgcag gtcgtcggtg agatggtacg ccatgttgca ggagcagagt ttatcagcga
     3241 acggcgcgag ttcggggaac gcctcctgtg ccgcctcccg gaggatgcgg tgactgcgcg
     3301 agccggtatt catgagcgtc gagtcgatgt cgaagacgca gagcggcctc ttaaccgccc
     3361 gaacgacatc gaacacctgt gctatggtca tcgtcatcag tggcctcccg cccgcccggg
     3421 cttggcgttc catatgcgga ggagcaggag catccctgcg acaccgaacg gcaggaggaa
     3481 gaccggccag aaggaccagt cgcgagtggt tatatagccg agcgacagcg actggaaccc
     3541 ggtgccgaga tagacaaagc cgtcgatgac cccgaccgcc gttgccgcac ccttccgacc
     3601 gccaaaatcc atcgttgcgg tccccgatag gagcccatgc gtcccgatga cggcgaccga
     3661 catgaagaac acaagcgcgc cgagtagcca gccgttggcg agcgacgcca gcatcgcggc
     3721 ggtgcagaga acaagagcgc cgtagaggaa cagcgccgcc ggtgcgcgcc gcgattggaa
     3781 gagtttatcc gacaccagcc ccgccacatt gccgccgatg ataccggcga ccatcaggag
     3841 caggccccag tggtcgagca tgaagccgaa cccggccgtc actcccgccg tgatctgttc
     3901 cttggcgtat atcgggaacc agtgcatgac gccgttgcgc aggacgccgg tgcagaattc
     3961 tatcgccgcg atggtcagta tcaccggatg ggtcagtatc cgtttgtaga gttgaagcgg
     4021 cgaaagtctc tcgtcgagat cctccccggc actcgcgtcg cccgtttcga agtcgctgaa
     4081 gcccgcctgc gccgggcggt cgcgcagaag gaaccactcg acggcgaaga tgaaaaggag
     4141 caacaccgcc ggcgagagga agacccacca cagcgcgtcg ccgccgttaa aaccctgccc
     4201 ggcaaccagc ggcgaggcga tgatggaccc cgtgacatcg aaagcgaggt agatgcccga
     4261 ggcgatcatg atgccgaaga tgcccgaaaa ggtaccgcgt tcggtgaggc cgaaccagtt
     4321 ggcgttcact ttgacaatgg agacggcacc ataactctgg aaatacatgt tgaccgcata
     4381 gagcacgata tagacagtgc gcag
//
LOCUS       DAOQUE010000227         4398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_22196_length_4398_cov_4.289892, whole genome shotgun sequence.
ACCESSION   DAOQUE010000227 DAOQUE010000000
VERSION     DAOQUE010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22196_length_4398_cov_4.289892"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PKH10_07750"
     CDS             <1..585
                     /locus_tag="PKH10_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28056.1"
                     /translation="SPDGSVSARATLILRGNYSSYIRQTLRTRKEADRFFQGLVNRLF
                     QGGTLIGAEYGDIEDIERPLTITLTADASNLLVRQGDRKRLRIPLDMLRADMIKLAER
                     VLPMELGIPGLTEVTVSVSLPPKTKVLSAPEKFRSDGPCFSVERTAQGGPNALTVTTR
                     YRKECTMLAPADYPAFRAAAQEVVSRQEDYIIFK"
     gene            complement(578..1129)
                     /locus_tag="PKH10_07755"
     CDS             complement(578..1129)
                     /locus_tag="PKH10_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28057.1"
                     /translation="MSQELIAVISLLEGALYQETKNLWDLCAERYGTKEVQLFQHPSF
                     TFQGGSVDTAKLPEMRKQFSRFAAKVKPFTLSVRKMGNAGKEMLYFEVERTEELAGTH
                     LMANAFLDIFCDRTIEEYLPERWHPHVALAMHDLSERGFTELWEDFGAVTYAFDQVIN
                     NLMLIRFLPDGDIEVLEKAKLTI"
     gene            complement(1133..2587)
                     /locus_tag="PKH10_07760"
     CDS             complement(1133..2587)
                     /locus_tag="PKH10_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT28058.1"
                     /translation="MRKDFRDIFRLTAFCVSACALLISCEGTFVRDGATPSGGDPSSG
                     DPYGSIFEQQEGTVEQGADHDYNGSTDTTYYPDDTYSDEVLSSDSSGGYSDGDPAPHS
                     DTATASDSYASSDSSVVPDSDPGSSEDGPEIPGESDIQPGQVTASEWNDLNNWEEWIA
                     LLDDEFSFYETAWGFVTRDRIPVQVVDGAGSPIADAETELSCEQEGIVWRARTDVAGH
                     ADLFSGVAGVPAAGPCKVVARYYEKSASSAKINSPVTTPIALTLNTVEQERDLLDLML
                     LFDTTGSMSDELEYIKAEMKDVILRVKENEAKQLRVRLSLNFYRDEGDEYVVLPFPFS
                     EDIDEAVTILAAQSANGGGDMPEAVHTAFLSAVDHHEWSAEARMRLLFWIHDAPPHEF
                     PEVIESLQGTIARAAAKGIRIIPVAASGADKNEEFVARSFDILTGGTYVFITDDSGIG
                     GEHGEPSGEVEAGVEYLNDLIVRLIGEYLSGENR"
     gene            complement(3093..4175)
                     /locus_tag="PKH10_07765"
                     /pseudo
     CDS             complement(3093..4175)
                     /locus_tag="PKH10_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
BASE COUNT          766 a         1335 c         1390 g          907 t
ORIGIN      
        1 tcgcctgacg gctcggtatc ggcgcgcgcg acgctgatcc tgcgcggcaa ctactcgtcc
       61 tacatcaggc agacgcttcg tacccgcaag gaggccgacc ggttcttcca ggggctcgtc
      121 aaccggctct ttcagggcgg gacgctcatc ggtgccgagt atggcgacat cgaggacatc
      181 gaacggccgc tcacgataac gctcacggcc gacgcgtcga atcttctggt gcggcagggg
      241 gaccggaagc ggctacgcat accgctcgat atgctgcgcg ccgacatgat aaaactggct
      301 gagcgggtcc tgccgatgga actcggcata ccggggctca ccgaggtgac ggtctccgtt
      361 tctctgccgc ccaagacgaa ggtgctctcg gcccccgaga aattccggag tgacggcccc
      421 tgtttcagcg tggaacggac ggcgcagggc ggcccgaacg cccttaccgt gacgacccgc
      481 taccgcaagg agtgcaccat gctcgcgccg gccgactacc cggcgttccg cgccgcggcg
      541 caggaggtcg tcagccgtca ggaagactac atcatattca aatagtcagt ttggcctttt
      601 ccaacacctc gatgtcgccg tcggggagaa agcgtatcag catgaggttg ttgatgacct
      661 gatcgaaggc gtaggtcacc gcgccgaagt cctcccagag ttcggtgaag ccacgctccg
      721 acaggtcgtg catcgccagc gccacatgcg ggtgccagcg ttccgggagg tactcctcga
      781 tggtgcggtc gcagaagata tcgagaaagg cgttcgccat gagatgggtc cccgccagtt
      841 cctcggtccg ttcgacctcg aagtagagca tctccttccc ggcgttcccc atcttgcgca
      901 ccgacagcgt gaaaggcttc accttcgccg cgaaccggga gaactgtttt cgcatctcgg
      961 gcagtttagc ggtatcgacc gagccgccct gaaaggtgaa ggatggatgc tggaacagtt
     1021 gcacctcctt cgtgccgtac cgctcggcgc agaggtccca gagattcttg gtctcctgat
     1081 agagcgcccc ttcgaggagc gatatgaccg cgatgagttc ctgagacatg agttagcggt
     1141 tctctcccga aaggtattcg ccgatgaggc ggacgatgag atcgttcagg tattcgaccc
     1201 ccgcctcgac ctcgccgctc ggttcgccgt gctcgccgcc gatgcccgag tcgtcggtga
     1261 tgaagacata ggtgccgccg gtgagtatgt cgaaggaacg ggcgacgaac tcctcgttct
     1321 tgtcggcgcc gctcgcggcg accgggatga tccggatacc tttcgccgcc gcgcgggcga
     1381 tggtgccctg cagactttct atgacctcgg ggaattcgtg gggcggcgcg tcgtgtatcc
     1441 agaagagcag gcgcatccgc gcctcggcgc tccactcgtg gtggtcgacg gcgctgagga
     1501 acgcggtgtg gaccgcctcg ggcatgtcgc cgccgccgtt ggccgattgc gcggcgagta
     1561 tcgtcaccgc ctcatcaata tcctcgctga acgggaaggg gagtaccaca tactcgtcgc
     1621 cttcgtcgcg atagaaattg agcgacaggc ggacgcgcaa ctgtttcgct tcgttctcct
     1681 tgacccgcag gatgacatcc ttcatctccg ctttgatgta ctcgagttcg tcgctcatcg
     1741 agccggtggt gtcgaacagg agcatgaggt cgagcaggtc gcgttcctgt tccacagtgt
     1801 tcagcgtcag cgctatcggc gtggtcacgg ggctgttgat cttcgccgag gaggcgctct
     1861 tttcgtagta gcgggcgacc accttgcagg ggccggccgc cgggaccccg gcgacgccac
     1921 tgaaaagatc ggcgtgaccc gccacatcgg tgcgggcgcg ccagacgatg ccttcctgct
     1981 cgcacgatag ttccgtttcg gcgtcggcta tcggtgaacc ggccccgtca acgacctgca
     2041 ccgggatgcg gtcacgcgtc acaaaacccc atgcggtctc gtagaaggag aattcgtcgt
     2101 ccagcagggc gatccactct tcccagttat tcaggtcgtt ccattcgctt gcggtgacct
     2161 gccccggctg tatgtcgctt tctcccggta tctccgggcc atcctccgaa gatcccgggt
     2221 cgctgtcggg taccaccgat gagtcactgg atgcgtagga gtcacttgcg gtcgcggtat
     2281 cgctatgggg ggccggatca ccgtccgaat agcctccgga agagtcggag gagagcactt
     2341 cgtcgctgta ggtgtcgtca gggtagtagg tggtgtccgt cgaaccattg tagtcgtgat
     2401 ccgccccctg ctcgaccgtt ccttcctgtt gttcgaagat actgccgtag gggtctccgg
     2461 acgaagggtc gcctcccgat ggcgtcgcgc cgtcgcgcac aaaggtccct tcgcaggaga
     2521 tcagcagggc gcacgccgaa acgcagaacg ccgtcaggcg gaaaatgtcg cggaagtctt
     2581 ttctcatgac ccgcctcctc aatgtcaagg ggtaacgaca ctattatggt gcgaggagcg
     2641 ggatactttt atcaaatggc ggataaaaaa gtagtaacca tacattatca cttgtctatt
     2701 tcttattggg cggttatggc gtcgcccgcc tgcaggtcgg cgaggcggtc ggagtattcc
     2761 tttttgagcg cggcggcccg ttcgagcgcc ttttcgtact cttcggcgaa ggcggcgcgc
     2821 ttctgctcgt aaccgttctt gaagtcggcg agtctctgtt cgtacccgtt gaccaatgcg
     2881 atccgtctct gctcgtagcc gttcttgaat tcggagacct tcagatcgta ggccgcttca
     2941 tactctttct ttgtttcggc gacccgtgcg agcgcggcgt cgtactcctc tttccattgg
     3001 gctatgcggg caccgatgag cgtctttctc tcgtcgatgg cggcggcgag ccgttccttg
     3061 aactttgcga actcctcctg atagtaggcg accatcgcct gttcgtactg ctccctgagt
     3121 tcgccgacca gttccttcac cggcacgatc ttttcgatgc gccacgcggt ggatccgcaa
     3181 aaggcaaagc cgtcccggag ttctcccttt cgtgcgttgt tgagcgcctc ggatatgcag
     3241 tattgcacct cgcgggcgtc gcacgattcg agacagcgcc acggacagtg gaaatccttg
     3301 cgctcgccgc gttcgacctc cttgaggaag tcgttgcgga tggctcgtcc cggcatcccg
     3361 accgggcttt ttatgatggt gatgtcatcc atcgcgcagt cgatgtaggc ctccttgaaa
     3421 cggatgtcag catcgcattc gtcggttgcg acgaagcggg tcgccatctg gacgcctgcg
     3481 gcgccgagtt ggaggatgcg ccggatatcg tcgcctgtat agacaccccc tcccgcgatg
     3541 accggtacgg ggcggtcgaa acgatgcgag aactcctgta cggcggccac cacttcggga
     3601 actatcttat cgagggcgta ggccgaatcg cccaactgtt ctatcttgaa gccgagatga
     3661 ccgcccgcct cggggccttc gaccacgacc gcgtcgggga tgtcatggta attcttctcc
     3721 cacgaccgga agatcagttt cgcggcgcgc cccgacgaaa cgatggggac caccttgacc
     3781 ttcgcggcgc gcagttgagt cagagggata tcctttatcg ggaggccggc gccgaggaag
     3841 acgatgtcgg gcttctcctc gatgacgacg cgcagcatat cctgaaaatt gttgaccgcc
     3901 accatcagat tgacgccgat gacgccggtg gagcgttcgc gtgccgagcg tatctcgcgg
     3961 cgcagggcgc ggatgttggc ctcgcgaccg ttgtgccggt aatcgggctc catgagccct
     4021 atggcgttgg cggcgatgac gccgatgccg ccttcgttcg cgacggcggc ggccagtccg
     4081 gcgagcgata tgccgacccc catgcctccc tgcacgaggg gtagggtggc ggtcgagtca
     4141 ccgatggtca gcacgggcat cgagaagaaa cgcatgtgcg actccgaaca tcgttagggg
     4201 acgactctat actgaagagt gagcgataag caataaaaac ggaaagatgg ggagggttat
     4261 tcggcgggtg cttccgcggg tgccgggctt gccccggcgg agccttcggc ggagacgggt
     4321 tcggcggcag gggccggttc cggagcgggg gcagggcttg ccccggcgga gccttcggcg
     4381 gagacgggtt cagccgcc
//
LOCUS       DAOQUE010000228         4389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_22262_length_4389_cov_3.831564, whole genome shotgun sequence.
ACCESSION   DAOQUE010000228 DAOQUE010000000
VERSION     DAOQUE010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22262_length_4389_cov_3.831564"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="PKH10_07770"
     CDS             <1..1342
                     /locus_tag="PKH10_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28059.1"
                     /translation="YEKKTDYKDLQKKLASANKTLQNFEKSRESFIKAKTDPVEKEIA
                     SLNKSLDTSFKETKGKIDKETSEFKAKKDGERKGIEKKIAAIQKQKKDFQKKFDSMQK
                     AVMADRDKKVKQIEGRQKARDDQWAAEGKKRRAAFEAELDKKKKQLAALEKQMSDGAK
                     QSDKLREGLGKKIEQLKIDAEKKVRKIEEDSVKKAQQMDVQQEKDRQSVYARYEGLYK
                     QDRAKIEKEMKDIEAEMKRLVKERDGEQVRLKTTIEKLEKDIVEKRDQWEKAAKGRQA
                     SFEGELAKAGAREAAAKKEYDKRKSQIERDFLARLDGTAKKVVDKSANSSKKEYDIER
                     NRDVESTAVTKEINNLRIAVYVERAKGKMTDGDFKEARKLLYKALYYDRENAQLKAAF
                     AEMSKSVAAMFADASRLMTDDPDQAKSILRKLTKNLPASDAYFIKAKFLLLDEE"
     gene            1368..2264
                     /locus_tag="PKH10_07775"
     CDS             1368..2264
                     /locus_tag="PKH10_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28060.1"
                     /translation="MLRILLLCVMVSMGGCIAFKKDVDLAKAECLAENSAKQKVMEER
                     LRADIAALTDRLRKVEEKLNLEKTVQENKINLSFSTLDELKGTIRDISNRIDAIDVGA
                     NRSTADLAATVKRVAEQSDALAKEVALLKAEWEDQRPVEHVTIDKGGRVRLPEDAERA
                     FNELAKMSTDAALAVAVRDGWATYLRKFPGRHDCEAVFFTGETYVAEKSWNLAIEQFR
                     RIDTDHKGCPKHELSYIRVAQALLVLNKKDYARKVVLGMREIFPTTEYPKQVAELEKK
                     LGLPSRDAKKGDKKEKQQEKKK"
     gene            2261..3430
                     /locus_tag="PKH10_07780"
     CDS             2261..3430
                     /locus_tag="PKH10_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28061.1"
                     /translation="MRPKPLLLVAAVLLALATGAGCAAQIDREVPPEDAIFSVSDMKL
                     LDGGRYLLLLSSNFDRAYDYGRVSVYDLEQRKVVSSLLVGSLGGRMMLVDQETRLYVS
                     TREENRIHRIALMRDPQGLPWLVYDGDGTLDGASRGTRKEPYAMATAEDDSILLVSHL
                     RGGEVMAFLRGEGMEGDMEAGIFDTDPGVTDIILDDLHRVFLTVHRSAGFIGVLRLTA
                     RDDLSVSLALSRLPLPLPSVGVDIRDITASLVEPSVYYLTYRNRDADTGTETPMVLKT
                     ELVGTEGGFALEHRWSTALAGELGESALVPCGDGGEYLFTASPSGGSVSVIEATSGTV
                     VGKVSLDDCSPYQLFSRVDDPKKRLFVGCFSQSRVAVLKADCAADDFMTISETIP"
     gene            3427..>4389
                     /locus_tag="PKH10_07785"
     CDS             3427..>4389
                     /locus_tag="PKH10_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28062.1"
                     /translation="MRYYLFLFLLPVFWSLVSCDSEPEKRYLKMPVEARVVQKTLTCL
                     DGSSGYVALVTDNTDKGGRLKLLNGCTGMVDEEFSDGSEGDGGGLSVGAVTAGFDLLD
                     RGDGIIAAVAIPAERVLMMKRILPGFVYAQSEEESIPLAIMPRVVHAVAGFFLVYGDA
                     GPDDGWVLVDAEGTVFPQSLTTRFSDIACNGLYCLALEPEGGVPYLIEVQLVAAEPHL
                     TASATQLPIAIRSLAPLSGVSAIDGERFALWNRTVLTVVGGETPQVQGGFGIHGDIRI
                     TAVAAAHYIADRPYHALSDASSSQRRVIFEVEPDEDSLFEPTDSL"
BASE COUNT          995 a         1190 c         1422 g          782 t
ORIGIN      
        1 ctacgagaag aagaccgact acaaggacct gcagaagaaa ttggcgtccg ccaacaagac
       61 cctgcagaat ttcgagaaga gcagggagtc gttcataaag gcgaagaccg acccggtcga
      121 aaaggagata gcatcgctga acaaaagcct cgacacctcc ttcaaggaaa cgaaggggaa
      181 gatcgacaag gagacatcgg agttcaaggc caagaaagat ggtgagcgga aggggataga
      241 aaagaagata gccgccatcc agaaacagaa gaaggacttc cagaaaaaat tcgactccat
      301 gcagaaagcg gtcatggccg accgcgataa gaaggtgaaa cagatagaag ggcgccagaa
      361 ggcccgcgac gaccagtggg cggccgaggg gaaaaagcgg cgcgccgcgt tcgaggccga
      421 actcgacaag aagaagaagc aacttgccgc gctcgaaaag cagatgagcg acggagcgaa
      481 gcagtccgat aaactgcgcg aggggttggg caagaagata gaacaactca agatcgacgc
      541 cgagaaaaag gtccgcaaga tcgaagagga ttcggtcaag aaggcccagc agatggatgt
      601 gcagcaggaa aaggatcgac agtccgtcta cgccagatac gaggggctct acaagcaaga
      661 ccgcgccaag atcgagaagg agatgaagga tatcgaggcc gagatgaagc ggctggtgaa
      721 agaacgcgac ggcgagcagg tccggctgaa gacgacgata gagaaactcg aaaaggatat
      781 cgtcgagaaa cgcgaccagt gggaaaaggc ggcgaagggc cggcaggcga gtttcgaagg
      841 agaacttgcc aaggcgggcg ctcgcgaagc ggccgcgaag aaagaatatg acaagcggaa
      901 gagccagata gagcgcgatt tccttgcgcg tctcgacggg accgccaaaa aggtcgtcga
      961 caagtcggcg aactcatcga agaaggagta cgacatcgaa cggaaccgcg atgtcgagtc
     1021 gaccgccgtg accaaggaga tcaataatct gcgcatcgcg gtgtatgtgg agcgcgccaa
     1081 gggcaagatg accgacggtg acttcaagga ggcgcggaaa ctcctgtata aggcgctgta
     1141 ctatgatcgg gagaatgcgc agttgaaagc cgcattcgcc gagatgagca agtcggtcgc
     1201 ggcgatgttc gccgacgcga gccgcctgat gaccgacgat cccgatcagg cgaagagcat
     1261 cctgcggaaa ctcaccaaga acctgcccgc cagcgacgca tacttcatca aggcgaagtt
     1321 cctgctgctc gatgaagaat agcgttcttc cccgcggggt ccttcctgtg ctccggatac
     1381 tcctgctgtg tgttatggtg tcaatgggcg gttgtatcgc attcaaaaaa gatgtcgacc
     1441 ttgccaaggc cgaatgcctt gccgagaact cggcgaaaca gaaggtcatg gaggagcggt
     1501 tacgcgccga tatcgccgcg ttgaccgatc gcctgcggaa ggtggaagag aaactgaatc
     1561 tagagaagac cgtgcaggag aacaagataa acctttcgtt ctcgaccctc gacgagttga
     1621 aagggaccat ccgggacatc agcaaccgta tcgacgcgat agatgtcggg gcgaaccgtt
     1681 cgacggccga tctcgccgca acggttaagc gcgtggccga acagagtgat gcgctcgcca
     1741 aggaggtggc gctccttaag gccgaatggg aggaccagcg ccctgtcgag catgtcacga
     1801 tcgataaggg cggtcgggtc cgtcttcccg aggacgctga acgcgctttc aacgaactcg
     1861 ccaagatgag caccgatgcc gcgttggcgg tcgcggtgcg tgacgggtgg gccacctacc
     1921 tgcggaagtt cccgggccgc catgactgcg aagcggtctt tttcaccggc gagacgtatg
     1981 tcgcggagaa gtcgtggaat ctcgccatag agcagttccg ccgcatcgat accgatcata
     2041 agggctgtcc caagcacgaa ctgagttata tccgcgtggc gcaggcactg ctcgttctga
     2101 acaaaaagga ctatgcccgg aaggtcgtgc tggggatgcg cgagatattc ccgaccaccg
     2161 aataccccaa gcaggtggcc gaactcgaaa aaaagttggg tctgccgtcg agggatgcga
     2221 agaaaggcga taagaaagag aaacagcagg aaaaaaagaa atgagaccaa agcccctctt
     2281 gctcgtagcc gccgtactcc ttgccctcgc aacgggggcg gggtgcgccg cgcagataga
     2341 ccgcgaggta ccgcccgagg atgcgatctt ttctgtgagt gacatgaaac tccttgacgg
     2401 cggccgctac ctgttacttc tttcgagcaa cttcgatcgt gcctacgatt atggccgtgt
     2461 ctcggtgtac gacctcgaac aaaggaaggt cgtatcctcc ctgctcgtgg gatctctcgg
     2521 cggccgcatg atgctggtcg atcaggagac acgactgtac gtgtcgaccc gagaggagaa
     2581 ccgcatacac cgtatcgccc tgatgcgcga tccgcaggga ctcccgtggt tggtgtacga
     2641 tggtgacggt acgcttgacg gcgcttcgcg gggaacgcga aaggagccgt acgcgatggc
     2701 gaccgccgag gacgacagca tcctgctggt cagccacctg cgtggtggcg aggtgatggc
     2761 ctttcttcgc ggtgagggca tggaagggga catggaggcg gggatcttcg ataccgatcc
     2821 gggggtcacc gacatcattc tcgacgatct gcaccgggtc tttctgacgg tccacagatc
     2881 ggcggggttc attggcgtcc tgcgcctgac cgcgcgggat gatctgtcgg tctcgcttgc
     2941 gctttcgcgg ctgcccttgc cgttgccgtc ggtgggggtc gacatccgcg acatcaccgc
     3001 ctcgctcgtc gaaccgtcgg tctattacct gacctaccgg aaccgtgacg cggataccgg
     3061 cacggaaacg ccgatggtcc ttaagacgga actggtcggt acggagggag gttttgcgct
     3121 ggagcaccgg tggtccacgg cgcttgccgg tgaattgggg gaatcggcgc tcgttccctg
     3181 cggagacggc ggtgagtacc tcttcaccgc ttcgccgagt ggcgggagcg tcagcgtgat
     3241 cgaagcgacg agcggcacgg tcgtcgggaa ggtctcgctt gacgattgtt cgccctatca
     3301 gctgttctcc cgggtggatg acccgaaaaa acggctgttc gtcggctgtt tctcgcagag
     3361 cagggtggcg gtattgaagg ccgattgtgc cgccgatgac ttcatgacga tcagtgagac
     3421 gataccatga gatactacct ttttttgttc cttctgcccg tgttctggtc gctcgtttcc
     3481 tgcgacagtg aaccggagaa gcgttatctt aagatgccgg tcgaggcgcg ggtcgttcag
     3541 aagaccctga cctgtctgga cggttcttcc ggctatgtgg cgctggtgac cgacaacacc
     3601 gacaagggag gacggttgaa actcctgaac ggatgcaccg gcatggtcga cgaggagttc
     3661 tcggacggtt ctgaagggga cggaggaggg ttgtcggtcg gtgcggtgac cgccggtttc
     3721 gacctgctgg atcgggggga tggtatcatc gccgccgtcg cgatcccggc ggaacgggtg
     3781 ctcatgatga aacgcatcct gcccggcttc gtctacgcgc agagcgaaga ggaatcgatc
     3841 cccctcgcca tcatgccgcg tgtcgtgcat gcggttgccg gtttcttcct cgtgtacggt
     3901 gacgccggtc ctgacgatgg ctgggtgctt gtcgacgcgg agggaacggt gttcccgcag
     3961 tcgctgacga cccggttctc cgatatcgcc tgcaacggac tctattgcct cgcgttggaa
     4021 ccggaagggg gcgtgcccta tcttatcgag gtccaactgg tcgccgccga gccgcatctt
     4081 accgcttcgg cgacccaact accgatcgcc atccggtcgt tggccccgct ttccggggtg
     4141 tccgcgatcg acggggagcg tttcgcgctc tggaaccgga ccgtcctgac cgttgtgggg
     4201 ggcgagaccc cgcaggtgca aggcggtttc ggtatccatg gcgacatccg gatcaccgcg
     4261 gtcgcggcgg cgcattacat agcggaccgt ccgtatcatg ccctttcgga tgcctcttcc
     4321 tcccagcgcc gggtcatttt cgaggtcgag cccgacgagg acagcctctt cgaaccgacc
     4381 gactcgctg
//
LOCUS       DAOQUE010000229         4312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_22817_length_4312_cov_4.972046, whole genome shotgun sequence.
ACCESSION   DAOQUE010000229 DAOQUE010000000
VERSION     DAOQUE010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22817_length_4312_cov_4.972046"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKH10_07790"
     CDS             <1..349
                     /locus_tag="PKH10_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNT28063.1"
                     /translation="YGMGSTTWEVTHKVTLRYGIQGIIGACFLGLGRALGETMAVTFV
                     IGNAHRISPSLYDAGNSIASTLANEFAEASEPIYLHSLIYLGLVLLVMTFFIQLAAQW
                     WVARLKKSMGAGL"
     gene            346..1194
                     /gene="pstA"
                     /locus_tag="PKH10_07795"
     CDS             346..1194
                     /gene="pstA"
                     /locus_tag="PKH10_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNT28064.1"
                     /translation="MKKADNARRRETLDIAMRVVSGAASLIGIFFLGWILWTVARQGL
                     AAFNIDFFTELPAPPGVEGGGLANAIAGTAIITLLATLMGVPLGLFTGVYLSEFGKST
                     RFGGMVRFTMNITMGVPSIIVGLFVYTLLVLPFKHFSAYAGAVALAIIMLPVVARTTE
                     DMLNLVPNALRESALAIGMPRWRMIIAILFRAARSGLLTGILLAVARVSGETAPLLFT
                     AMSSPYWMKGLAEPTANLTVTIFNYAMSPFDDWKQLAWGASLFIMATVLGVNILARTL
                     LRERTK"
     gene            1224..1988
                     /gene="pstB"
                     /locus_tag="PKH10_07800"
     CDS             1224..1988
                     /gene="pstB"
                     /locus_tag="PKH10_07800"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014028703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNT28065.1"
                     /translation="MADPTPKISVRGLDFFYGDHQALFSNDIDIAPNRVTAVIGPSGC
                     GKSTHLRVYNRIFELYREQRVKGAVLLNGENIFAPTVKLLDLRRRVGMIFQKPTPFPM
                     SVFENVAYGIRLHYAVSKSELDGRVEEALKGAALWEEVRDLLNRPGTALSGGQQQRLC
                     IARAIAVEPEVLLMDEPTSAIDPIATAKIEELVEGLKGKYTIVIVTHNMQQAARISDF
                     TAFFFKGYIIEFGATKQIFTNPHNKKTEEYITGRFG"
     gene            2000..2674
                     /gene="phoU"
                     /locus_tag="PKH10_07805"
     CDS             2000..2674
                     /gene="phoU"
                     /locus_tag="PKH10_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNT28066.1"
                     /translation="MTMRIQRDIAKLKQKLLEMSVRVEEVFSRTIRCVKERDAAAARL
                     LIDGDRDIDLAEVDLEEECLKTLALNQPVASDLRLLVTVLKINNDLERIGDLAVNIAE
                     RVLTLANHTEVPLPFDFNRMSGIAQVMLHRSIEALVNLDKTTAYEVLAQDREIDTIHR
                     EMYRTASKEMCAKPEQIEFLLHGIAVSRYLERIADHVTNIAEDVIYLVDGEIVRHHTE
                     EGPRPT"
     gene            2671..>4312
                     /locus_tag="PKH10_07810"
     CDS             2671..>4312
                     /locus_tag="PKH10_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNT28067.1"
                     /translation="MRRRLIWRLFPAFAAVVVTAIFLFSFTAFRIFDHFHLARVTEEL
                     KAQAALAEPILRTAPDRQQAARELGERTGTRFTVILPDGTVAADSAEDPAQMANHGDR
                     PEVKSAFAGLVGVSSRYSPTLQKNLLYVALRVREEGRTVFILRAAKPLARVKEAVMPI
                     LGVILFAALLIATAAVVASYLVARWMSIPLRYLQEGAGRFAMGELDRRLPLPETEEFA
                     QLARSLNAMAEELGDRIRTITGQRNELETLLTSMEEALVVVNGDGLVTRLNRAAGALF
                     HVAPDEAAGKPAIAIVRNRALNDLIGEVLATGQAGQREVELYDSDGRVLMAHGTIVPG
                     AERTALFVFNDVTRLKKLENIRKEFVANVSHELRTPITSIKGYVETLRDGAINDPANT
                     RAFLDTIARQSDRLGAIIEDLLALSKIERETEQGGIVRQPTALRGVMEAAVQPFRERA
                     AARGIRIVTTGDGGLTIPANGALLEQALVNLIDNAVKYSSDNGQITMEARRTGAEATI
                     SVADTGVGIPSEHLPRLFERFYRVDKARSRTVGGTGLGL"
BASE COUNT          852 a         1355 c         1348 g          757 t
ORIGIN      
        1 ctacggcatg ggctcgacga cctgggaggt gacgcataag gtgacgctac gctacggcat
       61 tcaggggatc atcggcgcct gcttcctcgg actcggccgc gcgctcggcg agacgatggc
      121 ggtcaccttc gttataggta acgcacaccg catctcgccg tcgctctacg acgcaggtaa
      181 ctctatcgcc tcgacgctcg ccaatgagtt cgccgaggcc tcggagccga tatacctcca
      241 ctccctcatc tatctgggat tggtgcttct cgtgatgacc ttcttcatcc aactcgccgc
      301 ccaatggtgg gtcgcccgcc tgaagaaaag tatgggggcg gggctatgaa gaaggccgac
      361 aacgcgcggc gccgcgagac gcttgatatc gcgatgcgcg tcgtttccgg ggccgcgtcg
      421 ctcatcggta tcttctttct cggctggata ctgtggaccg tcgcgcggca gggacttgcg
      481 gcgttcaaca tcgatttctt caccgaactg ccggcgccgc cgggcgtcga agggggcggg
      541 ctcgccaacg ccatcgcggg aacggcgatc atcacgctcc tcgcgaccct catgggcgta
      601 ccgctcggac tcttcaccgg cgtctatctc tcagagttcg gcaagagcac ccggttcggc
      661 gggatggtcc ggttcactat gaacatcacg atgggcgttc cttccatcat cgtgggactc
      721 ttcgtctaca cgctgctggt gcttcccttc aaacacttct cggcctacgc cggcgccgtt
      781 gcgctcgcca tcatcatgct accggtcgtc gcacggacga ccgaggacat gctgaacctt
      841 gtccctaacg ccctgcgcga atcggcgctc gccatcggga tgccgcggtg gcgcatgatc
      901 atagcgatac ttttccgagc cgcccggtcg gggctcctca ccggcatcct gctcgccgtg
      961 gcgcgggtat cgggcgagac cgccccgctc ctgttcaccg cgatgagcag tccctactgg
     1021 atgaaagggc tcgccgaacc gaccgcgaac ctgaccgtca cgatattcaa ctacgccatg
     1081 agcccgttcg acgactggaa acaactggca tggggagcct cgcttttcat catggcgacg
     1141 gtgctcggcg tgaacatact ggcccgcaca ctgctgaggg agaggacgaa atgagcgatg
     1201 tgaaactgcc gctgtatggc gagatggccg acccgacgcc aaagatatcg gtgcgcgggc
     1261 tcgatttctt ctacggcgac catcaggcgc ttttttccaa tgacatagac atcgccccga
     1321 accgcgtgac cgcggtcatc ggcccctcgg ggtgcggcaa atcgacccat cttcgcgtct
     1381 ataaccgcat cttcgaactg taccgcgaac agcgggtgaa gggagcggtc ctgctgaacg
     1441 gcgagaacat cttcgcgccg acggtgaaac tgctcgatct ccggcggcgg gtcgggatga
     1501 tattccagaa accgacgccg ttcccgatgt cggtcttcga gaatgtcgcc tacgggatac
     1561 ggctccacta cgcggtatcg aaaagcgaac tcgacggccg cgtcgaggag gcgctcaagg
     1621 gagcggcgct ctgggaggag gtgcgcgatc ttctcaacag accggggacg gcgctgtcgg
     1681 gcggccagca acagcggctc tgcatcgcgc gggccatcgc ggtcgagccg gaggtgctcc
     1741 tgatggatga accgacgagc gcgatagacc ccatcgcgac cgccaagata gaggaactgg
     1801 tggagggctt gaagggaaaa tacactatcg tcatcgtgac gcacaacatg cagcaggcgg
     1861 cccgcatcag cgacttcacc gcctttttct tcaagggcta catcatcgag ttcggggcga
     1921 ccaagcagat attcaccaac ccgcacaaca agaagaccga agaatacatc accggacggt
     1981 tcggataagg gaggaagcca tgacaatgcg gatacagcgc gacatcgcga agctgaaaca
     2041 gaaacttttg gagatgagtg tgcgggtcga agaggtgttc tcgcgcacca tccggtgcgt
     2101 caaggagcgg gatgcggcgg cggcccggct cctcatcgat ggcgaccgcg atatcgacct
     2161 cgccgaggtc gatctggaag aagaatgtct taagacgctc gcgctcaacc aaccggtggc
     2221 gagcgacctg cgcctgctcg tcacggtact caagataaac aacgatctcg agaggatcgg
     2281 cgatctggcg gtcaacatcg ccgagcgggt gctgacgctc gccaatcata ccgaggtgcc
     2341 gctgccgttc gatttcaacc ggatgtcggg catcgcgcag gtgatgctcc accgctccat
     2401 cgaggcgctc gtgaacctcg acaagacgac cgcgtacgaa gttcttgcgc aggaccggga
     2461 aatagatacc atacaccgtg agatgtatcg gacggcgtcg aaagagatgt gcgcgaaacc
     2521 ggagcagata gagttcctgc tgcacggtat cgcggtgtcg cgctatctcg aacggatcgc
     2581 cgaccatgtg accaacatcg cggaggatgt catctacctc gtggacggag agatcgtgag
     2641 acaccacacc gaagaaggac cgagaccgac atgaggcgac ggctcatctg gcgattgttc
     2701 cccgccttcg cggcggtcgt cgtgacggcg atattccttt tttctttcac cgcgttccgt
     2761 atcttcgacc atttccacct cgcccgggtc accgaggaac tgaaggcgca ggcggcgctc
     2821 gccgagccga tattgcgcac ggcacccgat cgccagcagg cggcccggga actgggtgag
     2881 cggaccggca cccggttcac cgtcatcctg cccgacggca ccgtagcggc cgattctgcc
     2941 gaggatccgg cgcagatggc caaccacggc gaccggcccg aggtgaagag cgccttcgca
     3001 gggctggtcg gcgtctcctc ccgctacagt ccgaccctgc agaagaatct gctgtatgtc
     3061 gccctgcggg tgcgcgagga gggtcggacg gtattcatcc tgcgcgccgc caaacctctt
     3121 gcgcgtgtca aggaggcggt catgcctatt cttggcgtga tcctgttcgc cgccctgctc
     3181 atcgcgaccg cggcggtggt ggccagttac ctcgtcgcac gatggatgag cataccgctc
     3241 cgttatctgc aggagggggc cgggcggttc gcgatgggcg aactcgaccg gcgactgccg
     3301 ctccccgaga ccgaggagtt cgcgcaactg gctcggtcgc ttaacgcgat ggcggaggaa
     3361 ctcggcgacc ggatacggac gataacaggg caacgcaacg agttggaaac gcttctcacc
     3421 agtatggagg aggcgctcgt cgtcgtgaat ggcgacgggt tggtgacccg gctcaaccgc
     3481 gccgccggag cgctgttcca tgtcgctccc gacgaagcgg cgggcaaacc ggccatagcc
     3541 atcgtccgca accgtgcgct gaacgacctc atcggcgaag tgctggcgac cgggcaggcg
     3601 ggccaacgcg aggttgaact gtacgacagc gacggacggg tgctcatggc tcacggcacc
     3661 atcgtgccgg gagcggaaag aacggctctt ttcgtcttca acgatgtgac gcgtctcaag
     3721 aaactggaga acatccgcaa agaattcgtc gccaatgtgt cgcacgaact gcgcactccg
     3781 ataacctcga tcaagggcta tgtcgagacg ctccgcgacg gagccatcaa cgatcccgcg
     3841 aacacccggg ccttcctcga caccatcgcg cgccagagcg accggctcgg cgccatcatc
     3901 gaggatctgc tcgcactatc caagatagag cgggagaccg aacagggcgg catcgtacgt
     3961 cagccgacgg ctctgcgggg ggtgatggag gcggcggtcc agcccttccg cgaacgggcg
     4021 gccgctcgcg gtatccggat cgtcacgacg ggcgacggcg ggctgaccat ccccgcgaac
     4081 ggcgcgctcc tcgaacaagc gctcgtcaac ctcatcgaca acgccgtcaa atattcaagt
     4141 gataacggac agataaccat ggaagcgcgg cggactggcg ccgaggcgac catcagcgtc
     4201 gccgataccg gcgtcggcat cccgtcggaa catctgccac gactgtttga gcggttctat
     4261 cgcgtcgaca aagcgcgcag tcgcaccgtc ggcggcaccg ggctggggct tg
//
LOCUS       DAOQUE010000230         4256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_23219_length_4256_cov_4.762438, whole genome shotgun sequence.
ACCESSION   DAOQUE010000230 DAOQUE010000000
VERSION     DAOQUE010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23219_length_4256_cov_4.762438"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1717
                     /locus_tag="PKH10_07815"
     CDS             <1..1717
                     /locus_tag="PKH10_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HNT28068.1"
                     /translation="PDVDIDIKKVHEPYADGEEQWFVETVIGNIAKMKGDRLPSSCFN
                     ADGSFPTATTKFEKRGVAIKLPKWISANCISCNQCSFVCPHAAIRPVLVNAEEKAGAP
                     KSFETIKPKGKAFEGYEYRMQVYPMDCQGCGNCADVCPAPKKALDMVFFDEIAEVEAQ
                     NNDFSIHIPVRDTIMKADSVTGSQYRQPLFEFSGACAGCGETPYVKLLTQLFGDRMVV
                     SNATGCSSIYGGTAPTTPYCTNKEGCGPAWANSLFEDNAEHGYGMALAIRQKRERIEM
                     LMKKLLELKCDCGDDCDCGDDCPCELAPETKKLFEEWIANKKSAEKTRELAPKIEEAL
                     TREACDDPMVDELLELADYFVKKSVWIIGGDGWAYDIGYGGLDHVLASGEDVNVLVLD
                     TEVYSNTGGQASKASQFGQVAKFAASGKKMRKKDLGLISMTYGYIYVASVAMGANQNQ
                     TLKAMIEAEQYDGPSIVIAYSPCINHGIDMTHSQKEMKLAVETGLWPLYRYNPTLKKE
                     GKNNFQLDMKEPTRDVQEIINSEVRFKTLEKQFPDEAKKLHQLLREDLKDRWERLKKL
                     AEHGF"
     gene            1717..3453
                     /locus_tag="PKH10_07820"
     CDS             1717..3453
                     /locus_tag="PKH10_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28069.1"
                     /translation="MIHERIAESSRPAGNSGPFFAAGWPHHLLLIFVSVFIAFYKLSF
                     GDLWTHLAQGRYILDHHTVPSTDVFSYTYFGKPWANWEWLHNLMTALCWEQLGPNGLI
                     LLRMLLLCGIALALYRLLRVYLASPLGSFALTCFSLVLLQQRVADRPHFFSYLCLALL
                     LLLLSRPRDSWRGPLLAVVALLIVWRNSHPSWVLGLAILAAWYGDTLLSSKDGPLRRS
                     WRLPILLVIGAVAIFLLTPIPIDLSAYLSLQSGETDVPEWRSLFFLGPALLNGYYLLF
                     FLFSALLLAAAVRSFRPRPFYTLLAVAMLANAYWHFRFIPEAVMLGMPLIAMALARTT
                     FPTPSPRRAALWALLLCTLTAFAAEHFRDDFNRKRGLGIDDTMVPLHAADFMKEHSLG
                     GNVYGHYWISMDFLMGYLYPAVKVAVDIRVPGLYPYDFARRFWGIESVNAFRAEVAPL
                     PVDLILLGHQETRTAGHIDRDLERMLLDEGADLVYFDRSSALLRTPRMADRTLRPFTV
                     LTRWNTDAAALERIAGDPALFARAVEELRMLRERTAGRDDFYREVLQGLYAKAPLSEE
                     QRHALEELYGER"
     gene            complement(3456..>4256)
                     /locus_tag="PKH10_07825"
     CDS             complement(3456..>4256)
                     /locus_tag="PKH10_07825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNT28070.1"
                     /translation="IGGEMQGTLNASLAQRQDVTTWIRQAVNNALEGMISFNTDARNG
                     DRYRILVEEEFSEGRRIPGGRVLYASYEGKVAGHREAFWYEESDPRSAYNAHYTVEGK
                     ALASSGMRLPVDRIHVTSTFGYRIHPITGRRGMHNGVDYGGRVGDPVYAVASGTVVTA
                     AQDPYSGKKIFLRHIDGTETCYLHLSAIGVRQGAPVKGGQLIGRVGNTGRSTGAHLHF
                     GIRQKGQWVNPLRIKMIAAPTLTGERFDRFKGQMRDIRALLEKTAASG"
BASE COUNT          777 a         1443 c         1232 g          804 t
ORIGIN      
        1 ccccgacgtg gatatcgaca tcaagaaggt ccacgagccc tacgccgacg gcgaagaaca
       61 gtggttcgtc gagacggtca tcggcaacat cgccaagatg aagggcgacc gcctgccgtc
      121 gtcgtgcttc aacgccgacg gttcgttccc gaccgccacc accaaattcg agaagcgcgg
      181 cgtcgcgata aaactgccca aatggatctc cgccaactgc atctcctgta accagtgctc
      241 gttcgtctgc ccgcacgccg cgatacggcc ggtactggtg aacgccgagg agaaggcggg
      301 ggctccgaag agtttcgaga ccatcaagcc caaaggcaag gcattcgagg gttacgagta
      361 ccgcatgcag gtctacccga tggactgcca gggctgcggc aactgcgccg atgtctgccc
      421 cgcacccaag aaggcgctcg acatggtctt cttcgacgag atagccgagg tcgaggccca
      481 gaacaacgac ttctcgatac atatcccggt gcgcgacacc atcatgaagg ccgactcggt
      541 caccggttcg cagtatcgcc agccgctctt cgagttctcg ggcgcctgcg ccggttgcgg
      601 cgaaacaccg tatgtaaaac tgctcaccca gctgttcggc gaccggatgg tcgtctccaa
      661 cgcgaccggc tgttcgtcga tctacggcgg caccgccccg accaccccgt actgcaccaa
      721 caaggagggg tgcggtccgg cctgggccaa ctcgctgttc gaggacaacg ccgaacacgg
      781 gtacggcatg gcgctcgcca tccgccagaa acgggaacgc atcgagatgc tcatgaagaa
      841 actgctcgaa ctcaagtgcg actgcggtga cgattgcgac tgcggcgacg actgtccgtg
      901 cgaactggcg cccgaaacga agaaactgtt cgaggaatgg atcgcgaaca agaagagcgc
      961 cgaaaagacc cgcgaactgg cgccgaagat agaggaggcg ctcacccgcg aggcgtgcga
     1021 cgacccgatg gtggacgagc tgcttgagtt ggccgactat ttcgtcaaga agtcggtctg
     1081 gatcatcggc ggcgacggct gggcctacga catcggctac ggcgggctcg accatgtcct
     1141 cgcctcgggc gaggatgtga atgtcctcgt gctcgacacc gaggtctatt cgaacaccgg
     1201 cggtcaggct tcgaaggcgt cgcagttcgg gcaggtggcc aaattcgccg cctccggcaa
     1261 gaagatgcgc aagaaggatc tcggcctgat atccatgacc tacggctaca tctatgtcgc
     1321 ctcggtcgcg atgggggcca accagaatca gacgctcaag gcgatgatcg aggccgaaca
     1381 gtacgacggc ccctccatcg tcatcgccta cagcccgtgc atcaaccacg gcatcgacat
     1441 gacccactcg cagaaggaga tgaaactcgc ggtcgagacc gggctgtggc cgctctatcg
     1501 ctataacccg acgctcaaaa aggaaggaaa gaacaacttc cagctcgaca tgaaggagcc
     1561 gacccgcgat gtacaggaga tcatcaacag cgaggtccgc ttcaaaacgc tcgagaaaca
     1621 gttccccgac gaggcgaaga aactgcatca actcctgcgc gaggatctca aggatcgctg
     1681 ggagcgtctc aagaaactcg ccgagcacgg gttctgatga tccacgaacg gatcgccgaa
     1741 agttcaaggc ccgctgggaa cagcgggcct ttttttgcgg ccggatggcc ccaccatctg
     1801 ttgctcatct tcgtttccgt cttcatcgcc ttttacaaac tctctttcgg cgacctttgg
     1861 acccatctcg cacaggggag gtacatcctc gatcaccata ccgtaccatc caccgatgtg
     1921 ttcagctaca cctatttcgg caagccgtgg gccaactggg aatggcttca caacctcatg
     1981 accgccctct gctgggaaca gctcggcccc aatggtttga tactcttgcg catgctcctt
     2041 ctctgcggga tcgccctcgc cctctaccgg ctcctgcggg tatatctcgc ctcaccgctc
     2101 ggctcgttcg cgctgacctg tttttctctc gtgctcctgc aacagcgggt cgccgatcgc
     2161 ccccacttct tctcctacct ctgcctcgcc ctcctgctgc tccttctctc gcgtccccgg
     2221 gactcgtggc gcggtcccct cctcgccgtc gtcgcgctgc ttatcgtctg gcgtaacagc
     2281 cacccgagtt gggtgctcgg cctcgcgatc ctcgcggcat ggtacggcga caccctgcta
     2341 tcgtcaaagg acggtcccct gcgccgttcg tggcgcctgc cgatccttct ggtcatcggc
     2401 gccgtcgcca tattcctgct cacgccgata cccatcgacc tttcggccta tctctcgctc
     2461 cagagcggag aaacggatgt cccggaatgg agatcgctct tctttctcgg tccggcgctt
     2521 ctcaacggct actacctgct tttcttcctc ttctcggccc ttctgctcgc cgccgccgta
     2581 cgatccttca ggccgagacc gttctacacc ctgctcgcgg tcgccatgct cgcgaacgcc
     2641 tactggcatt tccgcttcat tcccgaggcg gtcatgctcg gcatgccgct catcgccatg
     2701 gcgctcgcac gaaccacgtt cccgacgccc tctccccgtc gggccgcgct gtgggcgctt
     2761 cttctgtgca ccctgaccgc cttcgccgct gagcatttcc gcgacgactt caaccggaaa
     2821 cggggactcg gcatagacga caccatggtg ccgcttcacg cggccgactt catgaaggaa
     2881 cactctctgg gcggcaatgt gtacggacac tactggatat ccatggactt cctgatgggc
     2941 tacctctacc ccgccgtgaa ggtcgccgtc gacatccgcg tgccggggct ctacccgtac
     3001 gatttcgccc ggcggttctg gggcatcgag tcggtgaacg ctttccgcgc cgaggtcgcc
     3061 ccgttgccgg tcgacctcat cctgctcggt catcaggaga cccgcaccgc cggccacata
     3121 gaccgcgacc tcgaaaggat gcttctcgac gagggggcgg atctcgtcta cttcgaccgg
     3181 tcgagcgccc tcttacgcac cccgcgcatg gccgaccgga cgctccgccc cttcaccgtg
     3241 cttacccgct ggaacaccga cgccgcggcg ctggaacgga tagcgggcga cccggcgctc
     3301 ttcgcgcggg ccgtcgagga actacgcatg ctcagggaac ggaccgccgg acgcgacgat
     3361 ttctaccgcg aggtgctgca gggtctctac gccaaagcgc cgctcagcga agagcaacgg
     3421 cacgcgcttg aggaacttta cggcgaaaga tagcgtcagc ccgaagcggc ggtcttttcc
     3481 agcagggcgc ggatgtcgcg catctgtccc ttgaaccgat cgaaccgttc gccggtcagc
     3541 gtcggcgcgg cgatcatctt gatgcggagc gggttcaccc actgcccttt ctgccggatg
     3601 ccgaaatgga ggtgtgcccc ggtgctccgg ccggtgttac cgacgcggcc gatgagttgc
     3661 cctcctttca ccggcgcacc ctgccgcacc cctatcgcgc tgagatgcag atagcaggtc
     3721 tcggtcccgt ctatgtgcct gaggaagatc ttcttccccg aatagggatc ctgcgcggcg
     3781 gtcaccacgg taccgctcgc caccgcgtag accggatcgc cgacgcgccc gccgtaatcg
     3841 acgccgttgt gcatcccccg gcgaccggtg atggggtgga tgcggtaccc gaaggtcgag
     3901 gtgacatgga tgcggtcgac cggcaggcgc atgccgctcg acgcgagcgc cttgccttcg
     3961 acggtgtagt gcgcgttata ggccgacctc gggtcggact cttcgtacca gaacgcctcg
     4021 cggtgaccgg cgaccttccc ctcgtaggaa gcgtacagca cccggccgcc cgggatgcgg
     4081 cgcccttcgg agaactcctc ctcgacgagt atccggtacc ggtcgccgtt gcgggcgtcg
     4141 gtgttgaacg atatcatccc ctcgagcgca ttgttgaccg cctgccgtat ccaggtggtc
     4201 acatcctgcc gctgggcgag cgatgcgttg agcgtcccct gcatctctcc gccgat
//
LOCUS       DAOQUE010000231         4221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_23505_length_4221_cov_3.831493, whole genome shotgun sequence.
ACCESSION   DAOQUE010000231 DAOQUE010000000
VERSION     DAOQUE010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23505_length_4221_cov_3.831493"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="PKH10_07830"
     CDS             <1..1484
                     /locus_tag="PKH10_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT28071.1"
                     /translation="SEEGRERSELPWLLRRGDIYYEKKWYAADLANAEGLVERYRHHP
                     VAYYYLSDSWQGLGNTAKAAAVRQKAVRILPNYQPTLSRLADLYRELGKIPELLTTLR
                     HLLAIDPHHLGFNRMLGEAYLADGRWERALQIFQRALERNPEAAELWALAGDAAMLSG
                     ERVTAVTAFRRAWELAPESREYAERLDSVGETASDFFARYALSDGAVDEKIAAYRKAR
                     DTYPQKYAIVYDEGLQHLHYSGSVRSRFRMTIALNNEEGVREFATVTNYGRVVSARVV
                     KPDGTVIPAWRADAKNLYFLETAPGDVIDFVIESLQGPRSWLGGTDFRWFFASEGVYN
                     LHSRLVILAPKDLNLLFHLQGPATRTEPEDKDGAVIVFETRGTYQPSREEAMPPFMDE
                     VPFLAYTTVPSWEDFSRWQAVFLREQTGNSAEIERQVMALVQGKDADGRVQALRDWVA
                     RAIGYLSDDRGIDKVKPEKTTAVFTQRSGDCKDKALLLKLMLS"
     gene            1539..2282
                     /locus_tag="PKH10_07835"
     CDS             1539..2282
                     /locus_tag="PKH10_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28072.1"
                     /translation="MRGLPSMQFDHAVVYIPPQEGVATGYFVDPTASYDHYRALNPEI
                     EGTDAFVVDERTGRYEFVPLLSGLTASVALDIGPDGAAKLTLTGAAASNGRFRHAADG
                     DPFSYFSSLITRLSGAPVTVSQCTLASDAYAEPLEVTCAAQPFVPALAAALFGKLVEP
                     SERRYAALLGDRVGSWKVTVRGVAGPDTVWSVQNDLFEWRATPIEGGGVELTMELRRT
                     RIEPKEYAAFRSAVAVVMEKERALKGVQP"
     gene            2279..3889
                     /locus_tag="PKH10_07840"
     CDS             2279..3889
                     /locus_tag="PKH10_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT28073.1"
                     /translation="MTKVVSTFSAKGGTGTSVMTVNLAVYLAQKGKKVLLVDAAPNGG
                     TLHTYLNIPSWEVSRAVPDFFSILPLIDTDYPNLKFFSSLRSPQKGGNVSEYLVKWET
                     ELKKGAFDIMLIDMGSHLDHDLVATAQLSDFNILFVGADPVSIEKANYLFRLLFSTQL
                     EALVQRLDFNSVLQNVQRTRSEFLFSARNLLLALSEEAPKMRRHFAEMVHRLRIGVVF
                     NGVRTSADAELKDIYPLVIRNYYGFDLKSLGDVLFSDVFFSSSQGSAPMVVSERGGEF
                     IEMLDTIAGRLLSLISKRDPLQSAPFLPLTYYEMLGVQRGSSAQDIRAQYEKLRAVYF
                     SQSPLMREMFDTDNLFVYARLLESVYQNLMDPEIKREYDMSGGAVPETLEQSLPEGFD
                     LREVIRKYQRQKKSAHKLIKRDVFGREADPVPGTEEPVERREVNELFERYRGRTLTGA
                     DLKAFREELGITLKMVAEKTRISQTLLQAIESDDPHRMPAPAYLKGFLKSYCRTLRLS
                     DDNTERVVADIAERAVRPVAKEEGPTHA"
     gene            3882..>4221
                     /locus_tag="PKH10_07845"
     CDS             3882..>4221
                     /locus_tag="PKH10_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNT28074.1"
                     /translation="MLERFGDYQLIHKIATGGMAEIFLAKHVHSPATSMPIAIKRILR
                     NFSANQTFIKMFLAEARIICNIVHENIVRIYDFGKADESYYIAMEYVFGQNLGQLTNK
                     YLQKGKILPVE"
BASE COUNT          802 a         1354 c         1296 g          769 t
ORIGIN      
        1 catcggagga gggacgcgaa cgatccgaac tgccgtggct cctgcgccgc ggcgatatct
       61 attacgagaa gaagtggtac gccgccgacc tcgcgaacgc cgagggactg gtggagcgct
      121 accggcatca cccggtggcc tattattacc tttccgattc ctggcagggg ctggggaata
      181 ccgccaaggc ggccgcggta cgtcagaagg cggtccggat actgcctaac taccagccga
      241 cgctctctcg gctcgccgat ctgtaccgtg agctggggaa gatccccgaa ctccttacga
      301 ccctccgcca cctgttggcg atagacccgc accatctggg gttcaaccgg atgctcggcg
      361 aggcctatct ggcggacggt cgctgggaac gggccctgca gatcttccaa cgggcgctcg
      421 aacggaaccc cgaggcggcc gaactctggg cgcttgcggg cgacgccgcg atgctctccg
      481 gcgaacgggt caccgcggta acggcgttcc gacgtgcgtg ggaactggcg cccgagtcgc
      541 gcgaatacgc cgaacgactc gattcggtgg gggagacggc ttccgatttc ttcgcgcggt
      601 acgccctgtc cgacggggcg gtcgacgaga agatagcggc ctaccgcaaa gcgcgcgaca
      661 cctacccgca gaaatatgcc atcgtctatg acgagggact tcagcacctg cactacagcg
      721 ggagcgtccg ttcacggttc cgcatgacca tagcgctgaa caacgaggag ggggtgcgcg
      781 agttcgcgac ggtcaccaac tacggtcgcg tggtgtccgc ccgcgtcgtg aagccggacg
      841 gcacggtgat accggcatgg cgcgccgacg cgaagaacct ctattttctc gaaacggcgc
      901 cgggtgatgt catcgatttc gtcatcgagt cgttgcaggg gccgcgttcg tggctcggcg
      961 ggaccgattt ccggtggttc ttcgcctccg agggggtcta taatctccac agccgcctgg
     1021 tgatactcgc cccgaaagat ctcaacctcc tgttccatct gcagggaccg gcgacccgca
     1081 ccgagccgga ggacaaggac ggcgcggtca tcgtgttcga gacccgcggc acctaccagc
     1141 cctcccgcga ggaggcgatg ccgcccttca tggacgaggt gcccttcctc gcctatacca
     1201 ccgttccttc gtgggaggat ttcagccgct ggcaggcggt gttcctccgc gagcagaccg
     1261 ggaactccgc cgagatagag cggcaggtca tggcgctcgt gcagggcaag gatgccgacg
     1321 gccgggtgca ggcgctccga gactgggtcg cgcgggccat cggatacctt tcggacgatc
     1381 gcggcatcga caaggtcaaa ccggagaaga ccaccgcagt gttcacccag cggtcgggtg
     1441 actgcaagga caaggcgctc ctgctcaaac tcatgctctc ctaagccggc atcgagtccc
     1501 ggtacgcact ggtgaaaagc agggcgggag gcgcgctcat gcgcggtctg ccctccatgc
     1561 agttcgacca tgcggtcgtc tacatcccgc cacaggaggg cgtggcgacc ggctatttcg
     1621 tcgatccgac ggcgagttac gaccactacc gcgcgctgaa ccccgagata gaggggaccg
     1681 acgccttcgt cgttgacgaa aggaccggtc ggtacgagtt cgtgccgctc ctgtcggggc
     1741 tgacggcctc cgtcgcgctc gatatcggtc ccgacggcgc ggcgaaactg acgttgaccg
     1801 gcgcggccgc gtccaacggc aggttccgtc atgccgccga cggcgacccg ttctcctatt
     1861 tctcctcgct cataacgcgg ctgtccggcg ccccggtcac ggtctcccag tgcaccctcg
     1921 caagcgatgc gtacgccgag ccgctcgaag tgacctgcgc ggcccagccc ttcgtcccgg
     1981 cgcttgccgc cgctctgttc ggcaaactgg tcgaaccgtc cgagcggcgc tatgcggcgc
     2041 ttctgggcga tcgcgtcggc agctggaagg tcacggtgcg cggtgtcgcc ggccccgata
     2101 cggtgtggtc ggtacagaac gacctcttcg aatggcgcgc caccccgatc gaggggggcg
     2161 gcgtggaact gacgatggaa ctgcgccgga cgcgcataga gccgaaggag tatgcggcct
     2221 tccgttcggc ggtggcggtg gtgatggaaa aggaacgggc cctcaagggg gtacagccat
     2281 gacgaaggtg gtatcgacat tctcggccaa gggcggcacc ggcacctcgg tgatgacggt
     2341 caatctcgcc gtctatctcg cgcagaaggg caaaaaggtc ctgctcgtcg atgcggcccc
     2401 caacggcggc acgctacaca cctatctcaa catcccgagt tgggaggtct cccgcgccgt
     2461 tcccgacttc ttctcgatac tgccgctcat agacaccgac taccccaacc tcaagttctt
     2521 ctcgtccctc cgctccccgc agaagggagg gaacgtttcg gagtatctgg tgaaatggga
     2581 gaccgaactt aagaagggcg cgttcgatat catgctcatc gacatggggt cccacctcga
     2641 tcacgatctc gtggcgaccg cccaactgtc cgatttcaac atcctgttcg tcggcgccga
     2701 cccggtcagc atcgagaagg cgaactatct gttccgcctg ctgttctcga cccagttgga
     2761 ggctctggta cagcggctcg acttcaattc cgtattgcag aatgtccaga ggacccgatc
     2821 ggaattcctc ttctcggcgc gaaatcttct gctcgccctt tcggaggagg cgcccaagat
     2881 gcgccgtcat ttcgccgaga tggtgcaccg tctgcgcatc ggtgtcgtct tcaacggcgt
     2941 gcggacctcg gccgatgccg aactcaagga catctacccg ctcgtcatcc gcaactacta
     3001 cgggttcgac ctcaagagtc tcggcgatgt cctgttctcc gatgtgttct tctcctcttc
     3061 gcagggctcc gccccgatgg tggtgagcga gcgcggggga gagttcatcg agatgctcga
     3121 caccatcgcg gggcggctcc tgtcgctcat atcgaagcgc gatcctttgc agtcggctcc
     3181 cttcctcccg ctcacttact acgagatgct cggcgtccag cgcggttcga gcgcgcagga
     3241 catccgcgcc cagtacgaga aactccgcgc cgtctatttc tcgcagagtc ccctcatgcg
     3301 cgagatgttc gacaccgaca acctgttcgt ctacgcccgg cttctcgaga gcgtctatca
     3361 gaatctgatg gaccccgaga tcaagcgtga gtacgacatg agcggcgggg cggtccccga
     3421 gacactcgaa cagtcgcttc ccgaaggttt cgacctgcgc gaggtcatcc ggaaatacca
     3481 gcggcagaaa aaaagcgccc ataaactcat aaagcgcgat gtgttcggtc gcgaggccga
     3541 cccggtcccc ggcaccgaag agccggtcga gcggcgcgag gtgaatgaac tgttcgagcg
     3601 gtaccgcggt cgtacgctga ccggcgcgga cctcaaggcg ttccgggagg aactgggcat
     3661 aacgctcaag atggtggccg aaaagacccg catatcccag acgctcctgc aggcgataga
     3721 aagcgacgat ccgcaccgga tgcctgctcc ggcttatctc aagggcttct tgaagagtta
     3781 ttgccggacg ctccgtcttt ccgacgacaa caccgaacgt gtcgttgccg acatcgcgga
     3841 gcgggccgtc cggcccgtgg cgaaagagga gggccccacc catgcttgag agattcggcg
     3901 actaccaact catccataag atagcgaccg gcggcatggc cgagatattc ctcgccaaac
     3961 atgttcattc gcccgccacc agcatgccta tcgcgataaa gcggatcctg cggaatttca
     4021 gcgccaatca gacattcatc aaaatgttcc ttgccgaggc gcggatcatc tgcaatatcg
     4081 tccacgagaa catcgtccgg atatacgatt tcgggaaggc tgacgagtcg tactacatcg
     4141 ccatggagta tgtattcggc cagaaccttg gccagctcac caacaaatac cttcagaagg
     4201 gcaagatact tccggtcgag g
//
LOCUS       DAOQUE010000232         4163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_23949_length_4163_cov_3.628286, whole genome shotgun sequence.
ACCESSION   DAOQUE010000232 DAOQUE010000000
VERSION     DAOQUE010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23949_length_4163_cov_3.628286"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1820)
                     /locus_tag="PKH10_07850"
     CDS             complement(18..1820)
                     /locus_tag="PKH10_07850"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNT28075.1"
                     /translation="MRFIAVFLCAVGVLCGAVACKESPKTYELDFAFPEGDATVDEDT
                     LVTDVDTDTAPTVEEGIRSLLEEHLLFTNGVGVAVGVEDLRTGRRYAVAAGKSDTGEG
                     AALTADHLFRVASITKTFVAATVLRLMDNQKVKLSDTLDRWYPDYPEADRITVRMLLN
                     HTSGISDYIVVGEPEAIIARTALMPRLFEPGEGWSYSNTNYVLLGRIIEQVTGKTLGE
                     VLADEVFARGELTVTTLEKEQGLLGDLAAGHLYEENAPAPYTGLNPAWADGGIVSSVS
                     DLAHFAARLFGGWIISSAALDDMLTMVSTGSGGSYGLGLFSFESDAAKSGYGHNGSLV
                     NFNGLWAYFPQMRTAFAIQANYPTVMDSAFLLERIVALVANAEKAPVVDTCDHRDPRP
                     DTPAPDHELVRFKGIVHGIDAEDPATGAAHYYRTGETGGENLFCAEYAFRSDEGETPS
                     LYLIEECPEKRRLYTGETRVRRFELFIPLTLLAAAKESGEGTIESPLIDYSSIEYWYD
                     EATQRVTKRCQNGVPAGGGTMARVETCLDRNTDFSAGEEVRLFAYLPLSRDIEEEKQC
                     RCYNASGGVVSCPAIEDLFDCAVPEGYFAAAGDT"
     gene            1968..2441
                     /locus_tag="PKH10_07855"
     CDS             1968..2441
                     /locus_tag="PKH10_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28076.1"
                     /translation="MPKFFSLLLVLSVVACSGIDDIPYKGLFVNGSSYTLAIAPSGGQ
                     SWEPFTLAPGDEKRITVDEDRVLFTVSPAVVVWVFNEKESTVTFYDRDPNSDDDTLYC
                     LDGATACDGVTAIYYCNGGFWKRRPCADACVDLGYAAAVGCGYSSNAGKDQCFCE"
     gene            2393..3826
                     /locus_tag="PKH10_07860"
     CDS             2393..3826
                     /locus_tag="PKH10_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HNT28077.1"
                     /translation="MRLQFERREGPMLLRITLLALILSALPLAAADGDDGEKKEEPAK
                     FIFGSYGRVQPSTDLEGGSGRHTNIVSHGSRLEQPTYLELDLVYDFANKPDETDFHLV
                     TTLAFGESLFHDTGVFAALSTIRNLFIRAEGVGYKPLSLWAGSRMYRGDDIYLLDLWP
                     LDDVNIYGAGAGLRYTDTEVDIHFGLNRLETDWQVQKREVPSDTFGTETVEWMDRQRI
                     IVGAKATQYFLRKENSDFALKAKLFAEMQSIGSGTRISERSVNAATEEDRVTYPADIG
                     WSVGAQFGALGLGRNGFAHVMFKYSGGLAAYGTLTEPFGYDIDRKTTTAQEFLTALTG
                     NFEFWRMSLMIGSYARYFTDADPNRYDNDDGWEFILAVRPHIHIHDSLAFGAEVSHQL
                     RKPNGIAPDTAEQTLPMITKLSLFPIVFKGDSLSYGRPQVRLVYTVSFVNGDARATFP
                     EGDKRRDRQIQHFLGIGAEWWFNTEYR"
     gene            3827..>4163
                     /locus_tag="PKH10_07865"
     CDS             3827..>4163
                     /locus_tag="PKH10_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNT28078.1"
                     /translation="MKYLLSVCSLLLMACVDTGKVSGDIPITSNVTDWRDEVIYQLIT
                     DRFANGDINNDHNADPSNLAAWHGGDFQGIIDRIPYLKELGVTTLWISPVVKNVEEDA
                     GIAGYHGYWT"
BASE COUNT          763 a         1346 c         1278 g          776 t
ORIGIN      
        1 accatccgca ggtgggctta ggtgtcgccg gccgccgcga aatagccctc cggcaccgcg
       61 cagtcgaaca ggtcctcgat cgcgggacac gataccacgc cgccgctcgc gttgtagcag
      121 cggcactgct tctcctcctc gatatcgcgt gaaagcggca gataggcgaa caggcgcacc
      181 tcctcgcccg ccgaaaagtc ggtgttgcgg tcgaggcagg tctcgacacg ggccatcgtc
      241 ccgccgccgg cgggaacgcc gttctggcac cgcttggtca cccgctgggt cgcctcgtcg
      301 taccagtatt cgatgctgga atagtctatc agcggcgact ctatcgtacc ctcgccgctt
      361 tccttcgcgg ccgcgaggag cgtcaggggg atgaagagtt cgaaccgcct gacccgcgtc
      421 tcgccggtgt agagccgccg cttctcggga cactcttcga tgagatacag actgggcgtt
      481 tccccctcgt cggaacggaa ggcgtattcg gcgcagaaca ggttctcgcc gccggtctca
      541 ccggtccggt agtagtgggc ggcgccggtc gcggggtctt cggcgtcgat gccgtgaacg
      601 atgcctttga accggaccag ttcatggtcc ggggccggcg tatcgggacg cggatcgcgg
      661 tggtcgcagg tgtcgacaac cggcgccttc tcggcgttcg cgacgagcgc gacgatccgt
      721 tcgagaagga acgcgctgtc catcaccgtc gggtagttcg cctgtatcgc gaaggcggtc
      781 cgcatctgcg ggaaataggc ccacagtccg ttgaagttca caagcgaccc gttgtggccg
      841 tagccgctct tcgccgcgtc gctttcgaac gagaaaagcc ccagcccgta cgagccgccc
      901 gatccggtcg agaccatcgt gagcatatcg tccagcgccg cgctcgatat gatccagccg
      961 ccgaacaggc gcgccgcgaa atgggcgaga tcggagacgc tcgacacgat accgccgtcg
     1021 gcccacgccg ggttgagtcc ggtgtagggc gccggcgcat tctcctcgta caggtgcccg
     1081 gcggcgaggt cgccgaggag cccctgctcc ttttccagcg tggtgaccgt cagttcgccc
     1141 cgcgcgaaca cctcatcggc gagcacctcg ccgagcgttt tgccggtcac ctgttcgatg
     1201 atgcggccaa ggagcacata gttggtgttc gagtacgacc acccctcgcc cggctcgaac
     1261 agccggggca tgagcgcggt acgggcgatg atggcctccg gctcgccgac gacgatgtag
     1321 tcgctgatac cggaggtgtg gttgaggagc atccgtaccg tgatgcggtc ggcctccgga
     1381 tagtcgggat accaccggtc gagcgtgtcg gagagtttta ccttctggtt atccatgagc
     1441 cgcaggaccg tcgccgcgac gaaggtcttg gtgatgcttg cgacgcggaa gaggtggtcg
     1501 gccgtcagcg ccgccccttc accggtatcg ctttttccgg cggcgaccgc gtaacgcctg
     1561 ccggtgcgca ggtcctcgac gccgaccgcg acgccgacac cattggtgaa caacaggtgc
     1621 tcctcgagaa gcgatcgtat cccctcctcg accgtcggcg cggtgtcggt atcgacatcg
     1681 gtgacgaggg tgtcctcatc gaccgtcgcg tcgccttcgg gaaaggcgaa gtcgagttcg
     1741 taggtcttcg gggactcctt gcaggcgacc gcgccgcaca ggaccccgac cgcacacagg
     1801 aacaccgcaa taaaccgcat gatcgctttc ccctttataa aaacctcact tattatatac
     1861 aggtcgcggc ggatatcgag aaaagttttt agcccctctt atcttgcatt tttctccggg
     1921 tacgggtact atcggtgccg gtcatgcgct gtgagggagc atccgacgtg ccgaagttct
     1981 tttcgctact acttgttctt tcggtcgtcg cctgttcggg catcgacgac atcccctaca
     2041 agggtctctt tgtcaacggt tcctcctaca cccttgcgat agccccctcc ggcggccaga
     2101 gttgggagcc gttcacgctc gcgccgggcg acgaaaagcg gataaccgtg gacgaggatc
     2161 gggtgctgtt caccgtgtcg cccgccgtcg tcgtctgggt attcaacgaa aaggagagca
     2221 ccgtcacctt ctacgatcgc gacccgaaca gtgacgatga cacgctgtac tgtctcgatg
     2281 gagcgaccgc ctgcgacggg gtcaccgcga tctattactg caacggcggc ttctggaaac
     2341 ggcggccctg tgccgatgcc tgcgtcgatc tcggctatgc cgcggccgtc ggatgcggtt
     2401 acagttcgaa cgccgggaag gaccaatgct tctgcgaata acgctcctcg ccctcatcct
     2461 gtccgccttg ccgctcgccg cggctgatgg cgacgacggg gagaaaaagg aggaaccggc
     2521 gaaattcatc ttcggttcct acggtcgggt ccagccatcg accgatctcg aaggcggctc
     2581 cggcaggcat accaacatcg tatcgcacgg ctcgcggctc gaacagccga cctatctgga
     2641 actcgacctc gtctatgact tcgcgaacaa gcccgatgag accgatttcc acctcgtcac
     2701 gacgctcgcg ttcggcgaat cgctctttca cgacaccggc gtgttcgccg cgctgtccac
     2761 catccgcaac ctcttcatcc gcgccgaggg ggtcggctac aagccgcttt cgctctgggc
     2821 cgggagccgc atgtaccgtg gcgacgacat ctacctgctc gatctctggc cgctcgacga
     2881 tgtcaatatc tacggcgccg gggcgggact gcgttatacc gataccgagg tggatatcca
     2941 cttcgggctg aaccggctgg agaccgactg gcaggtgcag aagcgggagg taccatcaga
     3001 caccttcggc accgaaacgg tcgagtggat ggaccgccag cggatcatcg tcggggcgaa
     3061 ggcgacccag tacttcctgc gcaaggagaa ctccgacttt gcgctcaagg cgaaactgtt
     3121 cgccgagatg cagagcatcg gctccgggac ccgcatcagc gaacggagcg tcaacgcggc
     3181 gaccgaagag gatcgggtca cctaccccgc cgacatcggc tggtcggtgg gcgcgcagtt
     3241 tggcgcgttg gggctggggc gcaacgggtt cgcccatgtc atgttcaagt attcgggcgg
     3301 gctcgccgcc tacgggacgc tcactgagcc gttcggctac gacatcgacc gcaagaccac
     3361 cacggcgcag gaattcctga cggcgctcac cgggaatttc gagttctggc gaatgagcct
     3421 gatgatcggg tcctacgccc gttacttcac cgacgccgac ccgaacaggt acgataacga
     3481 cgacggctgg gagttcatcc tcgcggtgcg tccccatatc cacatccatg actcgctcgc
     3541 cttcggggcc gaggtgtcgc accaactgcg caaaccgaac ggcatagcgc ccgacaccgc
     3601 cgaacagacc ctgccgatga taacgaaact gtcgctgttc cccatcgtat tcaagggcga
     3661 ttcgctctcc tacggccggc cgcaggtgcg gctcgtctac accgtcagtt tcgtgaacgg
     3721 cgacgcccgc gccaccttcc ccgagggcga caaacggcgc gaccggcaga tacagcactt
     3781 cctcggcatc ggcgccgagt ggtggttcaa cacggagtac cggtagatga agtatctgtt
     3841 gtcggtatgc tctcttttgt tgatggcctg cgtcgacacc ggcaaggtat ccggcgacat
     3901 ccccatcacc agcaatgtca ccgactggcg cgatgaggtg atataccaac tcatcaccga
     3961 ccggttcgcg aacggcgaca tcaacaacga ccacaacgcc gacccgtcga acctcgccgc
     4021 gtggcacggc ggcgattttc aagggatcat cgataggata ccctacctca aggaactcgg
     4081 cgtcacgacg ctctggataa gcccggtggt gaaaaatgtc gaggaggacg ccggcatcgc
     4141 gggctatcac ggctactgga cgc
//
LOCUS       DAOQUE010000233         4157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_23996_length_4157_cov_3.783033, whole genome shotgun sequence.
ACCESSION   DAOQUE010000233 DAOQUE010000000
VERSION     DAOQUE010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23996_length_4157_cov_3.783033"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PKH10_07870"
     CDS             complement(<1..848)
                     /locus_tag="PKH10_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNT28079.1"
                     /translation="MDGYLSLVQFFFARYSFLAFPARMLAVIIVAYLLFFIGRHYILK
                     AIGYMVRRSSIDWDDLLFEKKVFHALIHLIPILVVHNAAYAFGGLEAAVRRLTSAAIV
                     LVMALFFSRLVAVLQALYNRTDGAQERPIKGLFQILSIAIYIVALILMAAVLMGKSPV
                     LILSGLGAMTAVLMLVFKDAILGFVASLQMSVSHSIRVGDWISVPQYGADGEVADVSL
                     YALRVRNWDNTITSIPTQKFLEDSFTNWRGMKESGARRISRSIPLDMNSVRFLSEAEI
                     AAIRAN"
     gene            complement(852..3233)
                     /locus_tag="PKH10_07875"
     CDS             complement(852..3233)
                     /locus_tag="PKH10_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28080.1"
                     /translation="MRHLFAVITGAALLLLGVSCSSGGSESCPAGYHWDDATHQCEPD
                     TSEPDNEQDIDTATDDSLPDDAVDELADTLVPDDDVYTGPCILAREGDAVAFDVKLHA
                     VTIGTITVGGVDDDAHVAGELWGKNEATGSDFKIATLVPSLSGTVIEFPAGTYKLFYR
                     GPKSAPTDPDNTMVDMDKKLTVTAAATLNIDLPLYVLQGSVTKNGAAFPALAAGEDTA
                     TSITFTSGSYSFTVPYAEFGAFTRVMPAGTYTVSFTGHLAGGAPLFTGRVYLGDTALK
                     LTEALSEPIDIVTTTVAGPVVMDGATVDSGVLAIVRNPPFESISLALISDLGADQNYS
                     AEVIANEGITYVVTYLSDIADYPYSFQKIAEWKDLAADSGSVALDMGRIHGTISLRTA
                     GGPFPALTSCTVDEPLCTRGKLKVMSIAAGTTTIKDLGISGDDYAYEALVLRRQRVCA
                     DIDCTTYDYAPLSFSLIFDGYFNDVAGMTNYLPFSVRLNNNGTENFQFTDGSGGFTTD
                     MTLDLVVVPTQVSGTVTYNGAALPETDDDLLFLRDVDTQTETPVARLKDLTGGAYSFF
                     APAASYHVVYKGASLLGYEQKGVVNTDLTVGDTDITAKIVDIGTAKMGLDLTVNGLSL
                     AELLTAYPAIDRYDFSAANDKNTLGSSVITATQAEPHPFFLVLRSPSWDLYLNLHVKE
                     GSDDSIFRFPIAQLVDVSADTVVQGDLKAVAFTGNLTANGAAVTGAASSRGFIELSGT
                     GSRAKAFFPPIGTQATRFFLAPQTYNTPTPQITVDDGFDVSLKIDAPCILVEE"
     gene            complement(3313..>4157)
                     /locus_tag="PKH10_07880"
     CDS             complement(3313..>4157)
                     /locus_tag="PKH10_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT28081.1"
                     /translation="LTVSAQALGIAQASFDEAVRYANEREQFGKKIIHFPAVFQMLKK
                     MQAEIETSRLLLYRTSLAVDYYDMYERKEHKGEDVKAPFKEAKALADFLTPLSKFTIT
                     ELANKASYDAIQIHGGTGYMKDFPVERLYRDARITNIYEGTTQLQVVAAIGTITSGLF
                     FKWMEAAKEIKADELSDAKKELIRHIARFAEVTERMKKVENKDFLDYVAGYLVELASI
                     IYRQYLFLPVAERHPEKRDLFKFFRNESEARIDHLIHQCDLAHRTYGEKISDLKNDLL
                     KKHE"
BASE COUNT          801 a         1203 c         1331 g          822 t
ORIGIN      
        1 tcattggccc gtatcgccgc gatctccgcc tcgctcagga agcgcaccga gttcatgtcc
       61 agcggtatgg agcggctgat gcggcgcgca ccgctctcct tcataccgcg ccagttggtg
      121 aaggagtctt caaggaactt ctgtgtcggt atcgaggtga tggtattgtc ccagttgcgg
      181 acccgaagcg cgtagagcga cacatcggcg acctcgccgt cggcgccata ctggggcacc
      241 gatatccagt cgccgacacg tatggagtgg ctcacactca tctgcagact cgcgacgaac
      301 ccgagtatgg cgtccttgaa caccagcatc aggacggcgg tcatcgcgcc gagccccgac
      361 aggatgagca ccggcgactt ccccatcagc accgccgcca tgagtatcag cgcgacgatg
      421 tagatcgcga tggaaagtat ctggaacagc ccctttatcg gccgctcctg cgccccgtcg
      481 gtcctgttgt agagcgcctg caggaccgcg acgagccgcg agaaaaatag cgccatgacc
      541 agcacgatgg cggccgaggt aaggcggcga accgccgctt cgagcccgcc gaaggcgtag
      601 gcggcgttat ggacgaccag tatcggtatg agatggatga gcgcgtggaa cacctttttt
      661 tcgaacagca ggtcgtccca atcgatagaa ctgcggcgca ccatgtaacc gatcgccttg
      721 agaatgtagt ggcgaccgat gaagaaaagc aggtaggcga cgatgataac cgccagcata
      781 cgcgccggga aggccaggaa ggaataccgg gcgaagaaga actggacgag cgaaaggtac
      841 ccgtccatcc cctactcctc gaccaatatg cagggggcat ctatcttcag cgagacatcg
      901 aagccgtcgt caacggtgat ctgcggggtc ggcgtgttgt aggtctgcgg tgcgaggaag
      961 aaccgcgtcg cctgcgtacc gatcggcggg aagaaggcct tggcgcggct cccggtcccc
     1021 gaaagttcga tgaagccgcg cgacgaggcg gccccggtga ccgcggcacc gttcgccgtg
     1081 aggttgccgg taaaggcgac cgccttaaga tcgccctgca cgaccgtgtc ggccgacaca
     1141 tcgaccagtt gcgctatcgg gaaacggaat atcgaatcgt cgctcccctc cttcacatgc
     1201 aggttgaggt agaggtccca actggggctt ctcagcacga gaaagaaggg atgcggctcg
     1261 gcctgcgtcg cggtgatgac ggacgagccg agcgtgttct tgtcgttcgc cgccgagaag
     1321 tcgtaacggt ctatcgccgg gtaggcggtc aggagttccg cgagcgacag gccgttcacg
     1381 gtgaggtcga gtcccatctt cgccgtccct atgtctacta tcttggctgt gatgtcggtg
     1441 tcgccgacgg tgagatcggt gttgaccacc cctttctgtt cgtagccgag gagcgacgca
     1501 cctttataga cgacatgata cgaggcggcg ggtgcaaaga acgaataggc gccccccgtc
     1561 aggtccttga ggcgcgccac cggcgtttcg gtctgggtat cgacatcgcg caggaaaagc
     1621 aggtcgtcgt cggtctccgg aagcgccgcg ccgttgtagg tgacggtgcc cgacacctgc
     1681 gtcgggacga ccaccagatc aagcgtcatg tcggtggtaa agccaccgga gccgtcggtg
     1741 aactggaaat tctcggtccc gttgttattc agccggacgc tgaacggcag gtaattggtc
     1801 atgccggcga catcgttgaa atagccgtcg aatatgagcg agaaggagag cggcgcgtag
     1861 tcgtaggtcg tgcagtcgat atcggcgcag accctctggc gccggagaac gagcgcctcg
     1921 taggcgtaat cgtcgcccga tatgccgagg tccttgatcg tcgtggtgcc ggcggcgatg
     1981 ctcatcactt ttaacttgcc gcgcgtgcaa agcggttcgt ccacggtgca ggaggtcagc
     2041 gccgggaagg ggccgcccgc cgtccgaagc gagatggtgc cgtggatacg ccccatgtcg
     2101 agcgcgaccg aaccgctgtc ggcggcgaga tccttccatt cggctatctt ctggaaactg
     2161 tagggatagt cggcaatgtc gcttagatag gtgaccacat aggtgatccc ctcgttggcg
     2221 atgacctcgg cgctgtagtt ctggtcggcg cccagatcgg atatcagggc gagcgatatc
     2281 gactcaaagg ggggattgcg cacgatggcg agtacgccgc tgtccaccgt cgcgccatcc
     2341 atcacgaccg ggccggccac cgtggtggtc acgatatcta tcggttcgct caacgcctcc
     2401 gtgagtttca gcgccgtgtc accgaggtag acgcggccgg tgaagagcgg ggcgccgccc
     2461 gccagatggc cggtaaaact caccgtgtag gtcccggcgg gcatgacccg ggtgaaggcg
     2521 ccgaactcgg catacggcac ggtgaaggag taactgccgg aggtgaaggt tatcgaggtc
     2581 gccgtgtcct cccccgccgc aagcgcggga aaggccgccc cgttcttggt gacgctcccc
     2641 tgcaggacat agagcggaag atcgatgttg agcgtcgcgg cggccgtgac ggtcagtttc
     2701 ttgtccatgt cgaccatggt gttgtcggga tcggtcggcg cgctcttggg tccgcggtag
     2761 aacagtttat aggtccccgc gggaaattcg atgaccgtgc cggaaagcga cgggacgaga
     2821 gtcgctatct tgaaatcgct gccggtcgcc tcgttctttc cccacagttc acccgcaaca
     2881 tgggcgtcgt catcgacgcc gccgaccgtt atggtgccga tggtcaccgc gtgtagtttc
     2941 acatcgaacg ccaccgcgtc gccttcccgc gcgaggatgc aggggccggt gtagacatcg
     3001 tcatccggca cgagcgtgtc ggcgagttcg tcgaccgcat cgtccggcag ggagtcgtcg
     3061 gtcgccgtgt cgatatcctg ttcgttatcg ggctcgctcg tgtcgggttc gcactggtgg
     3121 gtcgcatcgt cccagtggta accggcggga cacgactcgc taccgcccga ggagcaggaa
     3181 acgccgagca gcagcagcgc cgcgcccgtg ataacggcaa aaagatgacg catgcggacc
     3241 tccatcgttc cgttcaagga tgggccattc tagaaccttc ggggacggaa gtcaatgagg
     3301 gaacgggaga acctattcgt gttttttcaa caggtcgttc ttgaggtcgg atatcttctc
     3361 gccgtaggtg cggtgagcaa gatcgcactg atggatgaga tggtcgatgc gcgcctccga
     3421 ctcgttacgg aagaacttga agaggtcgcg tttttccgga tgccgctcgg cgaccggcag
     3481 gaacaggtac tgacggtaaa tgatcgatgc caactcgacg agatagccgg cgacataatc
     3541 gagaaagtcc ttattctcga ccttcttcat ccgctcggtc acctcggcga agcgtgcgat
     3601 gtgacgaatg agttcctttt tcgcgtcgga cagttcgtcg gccttgatct ctttcgccgc
     3661 ctccatccac ttgaagaaaa gcccggaggt gatcgtccct atcgccgcca cgacctgcaa
     3721 ctgcgtggta ccctcgtaga tgttggtgat gcgggcatcg cggtaaagcc gttcgaccgg
     3781 gaagtccttc atgtaaccgg tgccgccatg tatctgtatc gcatcgtagg aggccttgtt
     3841 ggccagttcg gtgatggtga acttggaaag cggcgtaagg aagtcggcga gcgccttggc
     3901 ctctttgaat ggcgccttga catcctctcc cttgtgctcc ttccgctcat acatatcgta
     3961 gtagtcgacc gctagactgg tacggtagag tagcaaccgc gaggtctcta tctcggcctg
     4021 catctttttg agcatctgga agaccgcagg gaagtggatg atcttcttgc cgaattgttc
     4081 ccgttcgttg gcgtatctga ccgcttcgtc gaaggatgcc tgcgcgatgc cgagcgcctg
     4141 cgccgacacg gtgagtc
//
LOCUS       DAOQUE010000234         4134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_24166_length_4134_cov_4.308899, whole genome shotgun sequence.
ACCESSION   DAOQUE010000234 DAOQUE010000000
VERSION     DAOQUE010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24166_length_4134_cov_4.308899"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PKH10_07885"
     CDS             complement(<1..732)
                     /locus_tag="PKH10_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HNT28082.1"
                     /translation="MRPLIPFIFSFFVLTVTGCFPTREGGPDTAVRPDDGTVLVEQDA
                     PTVDGEGLSPDDDEIPDLPDLSDASDTPDASDGSDLPDDTVTDDTPLPDEDLPPVAVF
                     FDDQVRARTLAELGRATEQVRFVSYTFSDDGVLAALNALHGKGVGVRGVTGTTITAGA
                     PLFPVRTYAESGDSGIVHTKFFVVDGATSLLSSSNIAYQDLRNFLVIIRDDPALAAAL
                     EEEFRQLDEEGRGTAKTRHCDPSCPT"
     gene            879..1991
                     /gene="gcvT"
                     /locus_tag="PKH10_07890"
     CDS             879..1991
                     /gene="gcvT"
                     /locus_tag="PKH10_07890"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNT28083.1"
                     /translation="MSQKTPLFDTHRELGATMGDFGGWNMPLWYKTGQLKEHVATRTA
                     CGLFDICHMGEFIVTGNDTLAFWDHLLTNAVTSMTDGQAMYNFMLNEQGGVVDDCIVY
                     RFSATRWMLVVNAGTQDGDHEWLKAHATGDVQIENIGPATGKLDLQGPTAPKVIAKLV
                     GREKLADLKFFRFIPEIMIGGVKCLLSRTGYTGEVGFEIYCPMGDTKKLWDILITVGA
                     EFGITPAGLGARDTLRLEAGLPLHGHEIAPGIPAVGTPWEFAINYEHEFIGKDALLAN
                     KGNLFVYPVIVEGRRKPGAHAQVLADGAACGEVVSDILAVSLDKKPAAFIRTTRELKS
                     GDTVTIRNEQGGSFTAQVMDNPLWRGTSRKKMDAFL"
     gene            2025..2429
                     /gene="gcvH"
                     /locus_tag="PKH10_07895"
     CDS             2025..2429
                     /gene="gcvH"
                     /locus_tag="PKH10_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNT28084.1"
                     /translation="MANYLVPGDLRYSKTHEWIKDLGGNKFRVGISDYAAKHIGDVTY
                     VELPQVDDEIGKEESGCVIETVKSSEDVYNSITGKVTAENGDILNDNPETINNDCYGE
                     GWLYEIQAASADDFKALMSPADYEKFLAELGD"
     gene            2497..3849
                     /gene="gcvPA"
                     /locus_tag="PKH10_07900"
     CDS             2497..3849
                     /gene="gcvPA"
                     /locus_tag="PKH10_07900"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNT28085.1"
                     /translation="MRYLPNTPAEVKEMLAAIGVNSIRDLFTCVPKNVLLKEPPALPA
                     AMSEMEIERHMAELGGKNEGAAMLSFLGGGCYRHYAPSLIDQMLLRSEFYTAYTPYQP
                     EASQGTLRAIFEFQTMIANLFSMDVSNASMYDGATAAAEAVMMALRNDRKKRTRIVVA
                     STLHPHYIQTLRTYLEGFTGEVVLMEFDQQTGRVADRELAKIDKDTVAVLFQYPNWFG
                     VIEDIHAIAKAAKAVEAWLIPAVIETTALGILTAPGELGADIAVGEGQPLGIPVNYGG
                     PGVGLFTTRTDHVRKMPGRLVGKTADREGKECFVLTLSAREQHIKREKATSNICSNQG
                     LMALAASMYLATMGKEGLRKVALTNLKRRDCLVKALAKTKAKLAFTGPCYNEIALKLP
                     MKADAVIDKMAAEGVLAGVPASRYYPALDDILITATTEFHSCADAGRFATTLAKVIGG
                     "
     gene            3851..>4134
                     /locus_tag="PKH10_07905"
     CDS             3851..>4134
                     /locus_tag="PKH10_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28086.1"
                     /translation="MSYTSNGFTDIKFHEEVLFERSRPGRAAYSLPAEDLTGAEDLLP
                     ADLRRKGLDLPELSEIDVVRHYTRLSTYNFSIVGIVNVPVRSLPVRVVVDV"
BASE COUNT          840 a         1292 c         1305 g          697 t
ORIGIN      
        1 ggtcgggcac gacgggtcgc agtggcgcgt cttcgcggtc ccgcggccct cctcgtcgag
       61 ttgccggaat tcctcttcga gcgcggcggc gagcgccggg tcgtcgcgga tgatgacgag
      121 gaagttgcga aggtcctgat aggcgatgtt ggatgacgac aggagcgagg tcgcgccgtc
      181 caccacgaag aatttggtgt ggacgatgcc gctgtcgccg ctctcggcgt aggtgcgcac
      241 cgggaagagc ggcgcgcccg ccgtgatggt ggtgccggtg acgccgcgca cgccgacccc
      301 cttcccgtgg agcgcgttga gcgccgccag tacgccgtcg tcggaaaagg tgtaggaaac
      361 gaagcggacc tgctcggtgg cgcgccccag ttcggcgagc gtccgggccc gcacttgatc
      421 gtcgaagaat acggcgaccg gcggcaggtc ctcgtcgggg agcggggtgt cgtcggtcac
      481 ggtgtcgtcg ggcagatcgg acccgtcgga tgcgtcgggt gtatccgacg cgtcggacag
      541 atcgggcagg tcgggtattt cgtcatcgtc gggagagaga ccctccccat cgacggtcgg
      601 tgcatcctgt tcgacaagaa cggtgccgtc gtcgggccgg acggcggtgt cgggtccccc
      661 ctcgcgggtg ggaaaacagc cggtcacggt caagacgaaa aaggaaaaaa tgaaaggtat
      721 caatggtctc acgcttgctc cccgtgcaca ccgaccgggc gaccataccc cgttcgggcc
      781 cgccggtcaa tactaacggc ggttgaacgg taaaaatctt gcaaaacggg tttcgctccg
      841 ttataagagg cgacgaaccg aaagaaaagg agcgtcccat gtcacagaaa acacccttgt
      901 tcgacaccca ccgggaactc ggcgccacga tgggcgactt cggcggctgg aacatgccgc
      961 tctggtataa gaccggtcaa ctcaaggagc atgtcgcgac ccgcaccgcc tgcggcctgt
     1021 tcgatatctg ccacatgggc gagttcatcg tcaccggcaa cgatacactc gccttctggg
     1081 accacctgct caccaacgcc gtcacctcga tgaccgacgg gcaggcgatg tacaacttta
     1141 tgctgaacga gcagggcggc gtcgtggacg actgcatcgt ctaccgcttc agcgcgaccc
     1201 gctggatgct cgtggtgaac gccgggacgc aggatggcga tcacgaatgg ttgaaagcgc
     1261 acgcgacggg cgatgtgcag atagagaaca tcggcccggc gaccggcaaa ctcgacctgc
     1321 aggggccgac ggcgcccaag gtgatagcga aactggtcgg cagggagaaa ctggccgacc
     1381 tcaaattctt ccgcttcatc cccgagatca tgatcggcgg ggtgaaatgc ctgctgtcgc
     1441 gcaccggcta caccggcgaa gtgggtttcg agatctactg cccgatgggc gacaccaaaa
     1501 aactgtggga catcctcatc acggtcggcg ccgagttcgg catcaccccg gcgggactcg
     1561 gggcgcgcga caccctgcgg ctcgaagcgg gactcccgct ccacggtcac gagatagcgc
     1621 ccggcatccc ggcggtcggc accccgtggg agttcgccat caactacgaa cacgaattca
     1681 tcggtaagga tgcgctcctt gccaacaagg gtaacctgtt cgtctatccg gtcatcgtcg
     1741 agggccgccg caaacccggt gcgcacgctc aggtccttgc cgacggcgcg gcgtgcggcg
     1801 aagtggtcag cgacatcctc gccgtttcgc tcgataaaaa gccggccgcc ttcatccgga
     1861 cgacccgcga actgaagagc ggcgacacgg tgacgatacg gaacgagcag gggggctcct
     1921 tcaccgcgca ggtgatggac aatccgctct ggagagggac ctcgcgcaag aagatggacg
     1981 cttttttgtg atagtatggg caactgatca acgaggagaa gaacatggct aactatctgg
     2041 tacccggcga tctccggtac agcaaaacac atgaatggat caaggacctc ggcggcaaca
     2101 agttccgggt cggcatcagc gattacgccg cgaaacacat cggcgatgtg acctatgtcg
     2161 aactgccgca ggtcgatgac gagatcggca aggaagaaag tgggtgcgtc atcgagacgg
     2221 tcaaatcgtc cgaggatgtc tacaactcga taacgggtaa ggtgaccgcc gagaacggcg
     2281 acatcctcaa tgacaatccc gagaccatca acaacgactg ctatggcgaa gggtggctgt
     2341 acgagataca ggcggcttcg gccgacgatt tcaaggcgct catgagcccc gccgattacg
     2401 agaaattcct cgcggaactg ggcgactagg cgcccttccc cgcgtacggt ccaccgggga
     2461 gggcaccgct ctcccctttt ttttacggag gccccgatgc gatatcttcc gaacacaccg
     2521 gccgaggtga aggagatgct cgccgccatc ggcgtcaact cgatccgcga cctgttcacc
     2581 tgcgtcccga agaatgtcct gctcaaagag ccgcccgccc tgcccgccgc gatgagcgag
     2641 atggagatcg agcggcacat ggccgaactg ggcgggaaga acgaaggcgc cgcgatgctc
     2701 tcgttcctcg gcggtggctg ttaccgccac tacgccccct cgctcatcga tcagatgctc
     2761 cttcgcagcg agttctacac cgcctacacc ccttaccagc ccgaggcgag tcaggggacc
     2821 ctgcgcgcca tattcgagtt ccagacgatg atcgcgaacc tcttttcgat ggatgtctcc
     2881 aacgcctcga tgtacgacgg cgcgaccgcc gcggccgagg cggtgatgat ggcgctccgc
     2941 aacgatcgca agaaacggac ccgcatcgtg gtcgcctcga cgctccaccc gcactacatc
     3001 cagacgctca ggacctacct cgagggcttc acgggcgagg tggtcctcat ggaattcgac
     3061 cagcagaccg gccgggtcgc cgacagggag ctggcgaaga tagataagga taccgtcgcg
     3121 gtgctgttcc agtatccgaa ctggttcgga gtgatagagg acatccatgc catcgccaaa
     3181 gcggccaagg cggtcgaggc gtggctcatc ccggcggtca tcgagaccac cgcactgggc
     3241 atcctgaccg ctcccggcga actgggcgcc gacatcgccg tgggcgaggg ccagccgctc
     3301 ggcatcccgg tcaactacgg cgggccgggc gtgggactgt tcaccacccg gaccgaccat
     3361 gtccgcaaga tgccgggccg gctcgtcggc aagacggccg accgcgaagg gaaggaatgc
     3421 ttcgtcctga cgctttcggc gcgcgaacag catatcaaac gcgagaaggc gacctcgaac
     3481 atctgctcca atcagggact catggcgctc gccgcctcga tgtacctcgc gacgatgggg
     3541 aaggaaggtc tgcgcaaagt ggcgctcacc aaccttaagc ggcgcgattg cctcgtaaag
     3601 gcgctcgcga agaccaaggc gaaactcgcg ttcaccggtc cttgttacaa cgagatagcg
     3661 ctgaaactgc cgatgaaggc cgatgcggtg atcgataaaa tggcagccga aggggtgctc
     3721 gccggcgtcc cggcgtcacg ctactatccg gcgctcgacg acatcctcat cacggcgacg
     3781 accgaattcc attcctgcgc cgacgccggg cgtttcgcca ccaccctcgc gaaggtcata
     3841 ggaggctgac atgagttata catccaacgg cttcaccgat atcaagttcc acgaggaggt
     3901 cctcttcgag cgctcgcgcc ccggtcgtgc cgcctattcg ctgcccgccg aggacctgac
     3961 cggtgccgag gacctgcttc cggccgacct gcgccggaag gggctcgatc ttcccgaact
     4021 ctccgagatc gatgtggtgc ggcactacac gcgcctctcg acctacaact tcagcatcgt
     4081 tggaatcgtg aatgttccgg ttaggtcctt gcctgtcagg gttgtggttg atgt
//
LOCUS       DAOQUE010000235         4127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_24239_length_4127_cov_4.555747, whole genome shotgun sequence.
ACCESSION   DAOQUE010000235 DAOQUE010000000
VERSION     DAOQUE010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24239_length_4127_cov_4.555747"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /gene="tig"
                     /locus_tag="PKH10_07910"
     CDS             <1..1252
                     /gene="tig"
                     /locus_tag="PKH10_07910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNT28087.1"
                     /translation="EKELSVTFPASEVAERLRNTFKRLSLTVTIPGFRPGKIPAAVIQ
                     QRYGKAVREETEQHLLESGLQEIAEKEKLRPFRYADINKSGPVADGAPFSFSCRVEVF
                     PDTGATVQELTVEWTPIPLREHLIDEEIESFRRRQVSFTAADRPAQEKDRATITFEGS
                     VDGEKRPGLSGADVPVLLGEKNFLPEIETALAGRRAGDKFDLTVNFPETFHAKDLAGK
                     AVDFAIEVRSIEEPHLPELTDELVAQHEPKAKTVAELRDQIRRDIERYLDELNIEQKR
                     HLTLDRYLKEYPFDLPPSLLSSEIAVREKEHLEKDPGAALSDEDKQKMARDAETAIRR
                     FILLSQLAETLSVTVSERDIEREIEKEAARYHIPVEHYKRYLDQGRWEQKRLALRDER
                     VIDKLVEKARFIEKPLDESRKEQP"
     gene            1261..1860
                     /gene="clpP"
                     /locus_tag="PKH10_07915"
     CDS             1261..1860
                     /gene="clpP"
                     /locus_tag="PKH10_07915"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNT28088.1"
                     /translation="MKHRPLSSLVPIVVEREGQSERAYDIYSRLLKDRIIFISGEITN
                     DLADLVVAELLFLESQDPAQDIQLYINSPGGLVTAGFAIYDTMQYVKPPVTTICIGQA
                     ASMGAILLASGTPGKRFALPNARVMIHQPLGGFHGQASDIEIHAKEILSIKERIIDIM
                     AKHTGADKKNLRRDMERDHFLSPEEAKAYGIIDQVITKK"
     gene            1857..>2306
                     /locus_tag="PKH10_07920"
     CDS             1857..>2306
                     /locus_tag="PKH10_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HNT28089.1"
                     /translation="MTSRNRRDTPIRCSFCGKPQEELMPNGRIIAGPGGVFICSDCVT
                     LCGEIVRDESAEPSTEGRAHLPLPSEIKRELDLHVIGQEYAKKVLSVAVYNHYKRIHR
                     GAAAADEVEIQKSNILLAGPTGCGKTLLARTLARTLNVPFAIADATTL"
     assembly_gap    2307..2316
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2317..3109
                     /gene="clpX"
                     /locus_tag="PKH10_07925"
     CDS             <2317..3109
                     /gene="clpX"
                     /locus_tag="PKH10_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNT28090.1"
                     /translation="NILLLLLQNCGFDVERASRGIVYIDEVDKIARKSDSPSITRDVS
                     GEGVQQALLKIIEGTVASVPPAGGRKHPQQEYIKIDTSNILFIAGGAFVGLDQIISRR
                     IGGSQLGFGRTPKKKTETAPDILAHLQPEDLLKFGLIPEFIGRFPVVGHLHELDESDL
                     LRILTEPKDALVKQFRALLNMDDITLTVHEEALREIVRQTATLKTGARGLRRVMEKMM
                     LDIMYAAPEEKDRVREIVITKAVVESGEPPLKILRHSTKKREEGA"
     gene            3323..>4127
                     /gene="rho"
                     /locus_tag="PKH10_07930"
     CDS             3323..>4127
                     /gene="rho"
                     /locus_tag="PKH10_07930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNT28091.1"
                     /translation="MNLQELKNLKNEAIVKMADDLGIENASSKRSQELIFEILQKLAD
                     RNEAITADGVLQILPDGFGFLRSPRYNYLPGPDDIYVPPAMIKRYYLRTGMTVEGDIR
                     SPKEGERYFALQKINSIFDETELDKIKHVPLFDNLTPFYPTEKIHLETRKNSVMSMRI
                     MDLLVPIGKGQRGLIVAPPRTGKTVLLQDIAHSVTANHPEVKLIVLLIDERPEEVTDM
                     MRSVAAEVVSSTFDEPATRHVQVAEMVLEKAKRMVEFKYDVVILLDSVTR"
BASE COUNT          964 a         1363 c         1159 g          631 t
ORIGIN      
        1 cgagaaggaa ctttcggtca ccttcccggc atccgaggtc gccgagcggc tccggaacac
       61 cttcaagcga ctctccctga cggtcaccat ccccggcttt cgtcccggca agataccggc
      121 ggcggtcatc cagcagcgct atggcaaggc agtgcgcgaa gagaccgaac agcacctgct
      181 ggaaagcggc ctgcaggaga tcgccgaaaa ggagaaactg cgcccgttcc gttacgccga
      241 catcaataag agcggcccgg tcgccgacgg cgcccccttc tcgttctcgt gccgcgtcga
      301 ggtgttcccc gacaccggcg cgacggtgca ggaactcacg gtggaatgga cccccatccc
      361 gctccgcgaa cacctcatcg acgaagagat cgaatcgttc cgccgccggc aggtatcctt
      421 cacggcggcc gaccggccgg cgcaggaaaa ggaccgcgcg accatcacct tcgaagggag
      481 cgtggatggc gagaaacggc ccggactctc cggcgccgat gtgccggtgc tgctgggcga
      541 gaagaatttc ctccccgaga tagaaacggc gctcgcgggg cgtcgcgccg gcgacaagtt
      601 cgacctcacg gtcaacttcc cggagacctt ccacgccaag gacctcgccg gtaaggcggt
      661 cgatttcgcc atcgaggtcc ggagcatcga ggaaccgcac ctgccggaac tcaccgatga
      721 actcgtcgcc cagcacgagc ccaaggcgaa aacggtcgcc gaactccgcg accagatacg
      781 gcgcgacata gaacgctatc tcgacgaact gaacatcgaa cagaaacggc accttaccct
      841 cgaccgttac ctgaaagaat atccattcga cctgccgccc tcgctcctga gttccgagat
      901 agcggtccgc gagaaggaac atctcgaaaa ggatcccggc gccgcccttt cggacgagga
      961 caagcagaag atggcgcgtg acgccgagac cgcgatacgc cggttcatcc tgctttcgca
     1021 actggccgag accctgtcgg tcaccgtatc ggagcgtgac atcgagcgcg agatagaaaa
     1081 agaggcggcc cgttaccaca tccccgtcga acactacaag cgttatctcg atcagggccg
     1141 ctgggaacag aaacggctcg ccctgcggga cgaacgggtc atcgacaaac tcgtcgaaaa
     1201 ggcgcgattc atcgaaaagc cgctggatga gagcaggaaa gaacaaccgt gaggataccc
     1261 atgaaacacc gacccctgtc atcgctcgtc ccgatcgtcg tcgaacggga gggccagtcg
     1321 gagcgggcat atgacatcta ctcgcgcctg ctcaaggacc gcatcatctt catcagcggc
     1381 gagataacca acgatctcgc cgatctcgtc gtcgccgaac tgctgttcct cgaatcgcag
     1441 gacccggcgc aggacatcca actgtacatc aacagccccg gcggactcgt caccgcgggc
     1501 ttcgccatct acgacaccat gcagtatgtc aaaccgccgg tcaccaccat ctgcatcggg
     1561 caggcggcga gcatgggggc gatcctgctg gcgagcggca cccccggcaa gcggttcgcg
     1621 cttcccaacg cccgcgtcat gatacaccag ccgctcggcg gctttcatgg acaggcgagc
     1681 gacatcgaga tacacgccaa ggagatactc tccatcaagg agcgcatcat cgacatcatg
     1741 gccaaacata ccggcgccga caagaagaac ctccgccgcg acatggaacg ggaccatttc
     1801 ctgtcgcccg aagaggcgaa ggcctacggc atcatcgatc aggtgataac gaagaaatga
     1861 cctcgcgcaa ccgccgggac acccccatcc gctgctcctt ctgcggcaaa ccgcaggagg
     1921 aactcatgcc caacggccgc atcatcgcgg ggccgggcgg cgtattcatc tgcagcgact
     1981 gcgtgacgct ctgcggcgag atcgtccgcg acgaatcggc cgaaccgtcg accgaaggcc
     2041 gcgcgcacct gccgctcccc tccgagataa agagggaact cgatctccat gtcatcgggc
     2101 aggaatacgc caaaaaggtc ctgtcggtcg cggtgtacaa ccattataaa aggatacatc
     2161 ggggcgccgc ggcagccgat gaggtcgaga tacagaaaag caacatcctg ctcgccggtc
     2221 ccaccggctg cggcaagacc ctgcttgccc ggacgctcgc ccggacgctc aatgtgccgt
     2281 tcgccatcgc cgacgccacc acgctcnnnn nnnnnngaac atcctgctcc tgcttttgca
     2341 gaactgcggc ttcgatgtcg agcgtgcctc gcgcggcatc gtctacatcg acgaggtcga
     2401 caagatagcg cgcaagtccg acagcccctc gataacccgc gatgtgtcgg gcgaaggggt
     2461 acaacaggcg ctcctcaaga tcattgaagg gaccgtcgcc agcgtcccgc ccgcaggcgg
     2521 ccgcaagcac ccccagcagg aatacatcaa gatagacacg agcaacatcc tgttcatcgc
     2581 cggcggcgcc ttcgtcgggc tcgaccagat catatcgcgc cgcatcggcg gctcgcaact
     2641 cgggttcggc cggacgccga agaaaaagac ggagaccgcc cccgacatcc tcgcccatct
     2701 tcagccggag gatctgctca agttcgggct catccccgaa ttcatcggac ggttcccggt
     2761 cgtcggtcac ctgcacgaac tggacgaatc cgaccttctg cgcatactca ccgagcccaa
     2821 ggacgcgctc gtcaagcagt tccgcgccct gctgaacatg gacgacatca cgctcaccgt
     2881 gcacgaggag gccctgcggg agatcgtgcg gcagaccgcg acgctgaaga ccggcgcgcg
     2941 cggcctgcgg cgcgtcatgg aaaagatgat gctcgacatc atgtacgcgg cccccgaaga
     3001 aaaggaccgc gtgagagaga tagttatcac caaggcggtc gtcgagtcgg gcgaaccgcc
     3061 gctcaagata ctcagacact caaccaaaaa aagagaggaa ggcgcgtagc caccctgttc
     3121 cgaccagtga aaaaaacacg gaaaatactt gacagcgcgc ccgggcgcga taggatgacc
     3181 ccgtacttac ggcgtatctc cataaggtcc acccccaacg gcaacaaacc gaacggtgcc
     3241 ttgaacgcac atgccgacac gcgaagtgtt aagaacgcat gacgagaaca accagcatac
     3301 ctgacaaggt aaggagcatt gtatgaatct tcaagaactc aagaacctca agaacgaagc
     3361 catcgtgaag atggcggacg acctcggtat cgagaacgcg tcttcaaaac ggtcgcagga
     3421 actcattttt gagatcctgc agaaactggc cgaccgcaac gaggcgatca ccgccgacgg
     3481 cgtcctacag atactgcctg acggtttcgg cttcctgcgc tcgccgcgct acaactacct
     3541 cccgggcccc gacgacatct atgtcccgcc ggcaatgatc aagcgctact acctgcgcac
     3601 cggcatgacg gtcgagggcg acatccgcag cccgaaggag ggtgaacgct atttcgccct
     3661 gcagaagata aacagcatct tcgacgagac cgaactcgac aagataaaac atgtcccgct
     3721 gttcgacaac ctgaccccct tctacccaac cgagaagata catctcgaga cgcgcaagaa
     3781 ttcggtcatg tcgatgcgca tcatggacct gctcgtgccg atcggtaagg gccagcgcgg
     3841 cctcatcgtc gcaccgccgc gcaccggcaa gacggtcctg ctgcaggaca tcgcccacag
     3901 cgtcaccgcc aaccatcccg aagtgaaact catcgtgctc ctcatcgacg aacggcccga
     3961 agaggtcacc gacatgatgc ggtcggtcgc ggccgaggtc gtctcctcga ccttcgacga
     4021 accggccacc cgccatgtgc aggtcgccga gatggttctt gaaaaggcca aaaggatggt
     4081 cgagttcaaa tacgatgtgg tgatactgct cgattcggtc acccgtc
//
LOCUS       DAOQUE010000236         4126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_24247_length_4126_cov_4.351511, whole genome shotgun sequence.
ACCESSION   DAOQUE010000236 DAOQUE010000000
VERSION     DAOQUE010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24247_length_4126_cov_4.351511"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PKH10_07935"
     CDS             <1..744
                     /locus_tag="PKH10_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNT28092.1"
                     /translation="PGLWGGLFASYLLIVSIYAFNSWAGLRDDAANPRLAAAGLTSGR
                     DYAVVSALTLATALTIFAFLRPAAVPYALGIFFLWGIYSFPRYGAKYVPFAGTIVHVA
                     VGMVQFQQGWALIGKPDDRSLFLSLYFALILAAGHVNHELIDHDADKTAGIASGAVRF
                     GPRLWTALHLAITLGALCLLLLLSFFGLFSFVWAAPFVTASALQSLSAATLVPTPYAS
                     RRMIVHRTRYRLCFLGAGLAFLMIRLTAS"
     gene            complement(731..2149)
                     /locus_tag="PKH10_07940"
     CDS             complement(731..2149)
                     /locus_tag="PKH10_07940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT28093.1"
                     /translation="MKIALVQPWPRDPLPGPLPASIGEGSEVMPPLGLLYLAGYLRER
                     SSHEPFVLDAWAERLNDADIVTRLHKAAPDIIGITAMTHTLRDALTVARAAKRAFPQV
                     PVVMGGPHPTLYPRETLALSGVDMVLAGEGERPLAMLLDAILSGGRLEEVPGLGWKKD
                     GVIVVNDECWSADPLDDLPFPAFDAVPVERYGSVLAVRRPVMSMVTSRGCPYRCSFCD
                     RPLLKGSRWRAHSAERVVAEMERLAGLGVREIALYDDTFTVDRQRVLDICRMKRERSP
                     SLLFSVRTRTDLVDEDVLAALAAAGCVAMHYGIESGSPPVRERIGKGSGIEEAITVCD
                     LTRRYGMETLAYFMIGNPGETPEDIAATERLIERLSPDYLHLTVLMPFPGTPIYREML
                     GKGRLAEDVWRRFAIDPSRDIEPPLWEEHLSRAQLLACRRRIYRRFYGRPGYILSQLR
                     GVSSLRELGAKAIAGIRLLTMR"
     gene            complement(2157..>4126)
                     /locus_tag="PKH10_07945"
     CDS             complement(2157..>4126)
                     /locus_tag="PKH10_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28094.1"
                     /translation="PFQMIFAISLTAAFSILFTGLGALLANRLRRRIILVMTMTGALF
                     FVSFLLISLGTLLPKSVMFAVTLDNVLALLPGGMVASIPLAAAAVAAPLVLSGGLAGG
                     FYLTILEEDRPLLRWLVAGIAVGFFVGYAGTAFLMGLTGIWNLILVVSALAAVGFLKP
                     LRFALAAFALIVTVLVADPDRYLFDALGREPLIWSQGGKPSRVAGFWSPYARLDFYDV
                     GEERLAGLYNGIQWWLTGDTTHDLSVRRELYRHIAGDVLIIGSGGGHGLLSLAGASSI
                     TAVELDPGVIETLKGPLAGYNDGVYNRVDTYAMEGRAFLDLTDRRFDVVVYEAPEFVF
                     ANSEKSFVSMENYLYTLEGIGRAFERLRPGGTLIVYHTYGLIPTERFVKAYPPGTRWA
                     VFRAEAELIRKVQVAFAVASREEATIERWREVFLRGGAEELSTTPAFAASLAAAAPVT
                     DDRPLLHFRDWRQALPFIVSALLVAVLLGLMLYRRTPRRLPIFFALVGVGFIMTELAV
                     INLLRSLLGGYVETTGVALGSLMLSSAVGALFHERMRSKWLVPLCIAGFVVMFLLLRY
                     PPVAAPWIVKVLWIGAAVALPGAVTGVFFPAGLAAGAAAESSYFYAVDTVATALGFLV
                     FYLVILAAGFTGAIATALLCYLVAARIFSRS"
BASE COUNT          797 a         1381 c         1271 g          677 t
ORIGIN      
        1 ccgggcctct gggggggtct tttcgcctcg tatctgctca tcgtttccat atacgcgttc
       61 aacagttggg ccgggctgcg cgatgacgcc gccaaccccc ggctggcggc ggcgggactt
      121 acctcgggcc gcgattatgc ggtcgtctcg gctctaacgc tcgcaacagc gttgactatc
      181 ttcgccttcc tgcggcccgc cgccgtcccg tacgcactgg gcatcttctt cctgtggggc
      241 atctattcgt tcccacggta cggtgcaaaa tatgtcccgt tcgccgggac catcgtgcat
      301 gtcgccgtcg gcatggtcca gttccagcag ggctgggcgc tcatcggcaa gcccgacgac
      361 cggtcgctat tcctttccct ttatttcgcg ctcatactcg ccgccggtca tgtgaaccac
      421 gaactgatag atcacgatgc cgacaagacg gcgggcatcg caagcggcgc ggtccggttc
      481 gggccacggc tctggacggc gctccacctc gcgataacgc tcggcgccct ttgtcttctg
      541 ctcctgctgt cctttttcgg gctgttctct tttgtctggg cggcgccttt cgtgacagcc
      601 tctgcccttc agtcgctttc ggccgcaacg ctcgtgccga cgccctacgc ctcacggcgg
      661 atgatcgtcc accgtacccg ttaccgtctt tgttttctcg gcgccggatt ggcgttcctg
      721 atgatacgcc tcaccgcatc gtgaggagcc ggatcccggc gatcgctttc gcccccagtt
      781 cgcgcaacga agacacgccg cgcaactgtg aaaggatata cccggggcga ccgtagaatc
      841 gacgatatat ccggcgacgg cacgccagca gttgggcgcg ggaaagatgt tcttcccaca
      901 atggcggttc gatatcacgc gacgggtcaa tcgcgaatcg ccgccacaca tcctccgcta
      961 atcgcccttt tcccagcatc tctcgataga tcggcgtacc cgggaacggc atcaacaccg
     1021 tcagatgcag gtaatcgggc gacagccgtt cgatcagacg ctcggtcgcc gcgatatctt
     1081 ccggcgtctc ccccgggttc cctatcatga aataggcgag cgtctccatc ccgtaccgtc
     1141 gcgtcaggtc acaaaccgtt atcgcctctt cgataccgct ccctttccct atccgctccc
     1201 tgaccggcgg cgatcccgat tcgatgccgt agtgcatcgc gacacacccc gcggcggcaa
     1261 gcgcggcgag cacatcctca tcaaccagat cggtacgggt ccggacgctg aaaaggagcg
     1321 acggcgaccg ctccctcttc atgcggcaga tgtcaagcac ccgttgccga tcgacggtga
     1381 aggtgtcgtc atacagcgcg atctcgcgaa cgccgagtcc ggccagccgt tccatctcgg
     1441 cgaccacccg ttccgcggag tgggcgcgcc accggctccc cttgaggagc gggcggtcac
     1501 agaaggaaca ccgataggga cagccgcgcg aggtgaccat gctcatcacc gggcggcgta
     1561 cggcgagcac cgacccgtac cgttcgacag gtaccgcatc gaacgcgggg aacggcaggt
     1621 catcgagtgg atcggccgac cagcattcat cgttcacaac gatgaccccg tcttttttcc
     1681 agccgagtcc cggcacctct tcgagtcgcc cgccggacaa gatcgcgtcg agtagcatcg
     1741 cgagcggtcg ctctccttca cccgcgagga ccatatcgac gcctgacagc gcgagcgtct
     1801 cccgtgggta gagcgtcggg tgcggacctc ccatgacgac gggaacctgc gggaacgccc
     1861 gtttcgcggc ccgtgcgacg gtgagagcgt cacggagcgt atgggtcatc gccgttatac
     1921 cgatgatgtc gggcgccgcc ttatgcaatc gtgtcacgat gtcggcatcg ttcagccgtt
     1981 ctgcccatgc gtccagcacg aacggctcat gcgatgaccg ttcccgcaga taacccgcca
     2041 ggtacagcaa tccaagcggc ggcatgacct cggaaccttc ccctatcgac gcaggtaagg
     2101 gccccggcag tggatcccgc ggccacggct ggacgagcgc tatcttcatg acggcctcac
     2161 gaccgcgaga agatgcgtgc cgcaacgaga tagcagagca gggccgtcgc gatggcgccc
     2221 gtgaaaccgg cggcgagtat gaccagatag aacacgagga acccgagcgc ggtcgccacc
     2281 gtgtccaccg cgtagaaata ggaggactcg gcggcggccc cggcggcgag gccggcgggg
     2341 aagaaaaccc ctgtgacggc gccggggagc gcgaccgccg cgccgatcca caggaccttg
     2401 acgatccacg gcgccgcgac cggcggatat cgcaggagca ggaacatgac cacgaaaccg
     2461 gcgatgcaga gcggcaccag ccacttcgac cgcatccttt cgtggaacag cgcgccgacg
     2521 gccgaggaaa gcatgagaga accgagtgcg acaccggtcg tttcgacata accgccgagc
     2581 agtgagcgca ggaggttgat gaccgcgagt tcggtcatga tgaagccgac cccgaccagc
     2641 gcgaagaaga tcggcaggcg acgcggcgtc cggcggtaca gcatgagtcc gagcaacacc
     2701 gccaccagca aggcgctcac gatgaacggc agggcctgtc gccagtcgcg gaaatggaga
     2761 agcggccggt catcggtcac cggcgcggcg gcggcgagcg acgcggcgaa tgcgggggtc
     2821 gtcgacagtt cctccgctcc gccccgcagg aacacctcgc gccaccgttc gatcgtcgcc
     2881 tcctcgcgcg aagcgaccgc gaaggccacc tgcaccttcc gtatcagttc ggcctccgcg
     2941 cggaacaccg cccaccgcgt accgggggga taggccttca cgaaccgttc ggtcggtatg
     3001 agtccatagg tgtgataaac gatgagcgta ccgcccggcc gcaaccgctc gaaggcgcga
     3061 ccgataccct ctagggtgta gaggtagttc tccatgctca cgaaactttt ctcggagttg
     3121 gcgaacacga actcgggcgc ctcatagacc acgacatcga accgccggtc ggtgagatcg
     3181 agaaaagcgc gcccctccat cgcgtaggta tccacgcggt tgtagacgcc gtcgttgtat
     3241 ccggcgagcg gacctttgag cgtctctatg accccggggt cgagttctac cgccgttatc
     3301 gaggatgcgc cggccaagga gagcaggccg tgaccgccac ccgatccgat gatcagcaca
     3361 tcgccggcga tatgacggta cagttcccgc cggaccgaca gatcatgcgt cgtgtcgccg
     3421 gtgagccacc actggatgcc gttgtagagc cccgccagcc gttcctcgcc gacatcgtag
     3481 aaatcgagcc gcgcgtaggg cgaccagaat cccgcgacgc gggagggctt tccgccctga
     3541 ctccagatga gcggttcgcg ccccagtgcg tcgaacagat accggtcggg atcggcgaca
     3601 aggaccgtta caatgagcgc gaacgccgcg agcgcgaacc ggagcggctt caggaagccg
     3661 accgccgcaa gcgcggaaac gacgagtatg aggttccata tccccgtcag tcccatgagg
     3721 aacgcggtcc cggcatagcc gacaaagaac ccgacggcga tccccgcgac caaccaccgc
     3781 aggagcggcc ggtcctcctc gagtatcgtg agatagaacc cgccggcgag tccgcccgac
     3841 agcacaagcg gcgcggcgac ggccgccgcc gccagcggta tgctcgccac catgccgccc
     3901 ggcaggagcg cgagcacatt atcgagcgtc acggcgaaca tgacgctttt ggggagcagg
     3961 gtaccgagcg atatgaggag gaacgaaacg aagaacagcg cgccggtcat cgtcatgacc
     4021 aaaatgatcc ggcggcgcag gcgattcgcc aaaagcgcac cgagaccggt gaacaggatg
     4081 gagaaggcgg ccgtcagcga tatcgcgaat atcatctgga acggga
//
LOCUS       DAOQUE010000237         4122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_24282_length_4122_cov_4.089009, whole genome shotgun sequence.
ACCESSION   DAOQUE010000237 DAOQUE010000000
VERSION     DAOQUE010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24282_length_4122_cov_4.089009"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PKH10_07950"
     CDS             <1..844
                     /locus_tag="PKH10_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28095.1"
                     /translation="DNTAWEHLRRTRLFLDEHLKTHPDTPPETREEALRAIYRAEGSD
                     WFWWYGPQFQTENDYLFDRLFRRHLRRVYAVLGAPHPTALDLPIKRSAGVTDNLTLPI
                     TTISPEITGRTDDFYGWAGAGLFDNTRRPGGVMYHGVRVVDRLWFGFDRDHLYFRVAL
                     AAPPVTQEHKRSAIRFYITDGMSLDLQLPIARFEKAFFPVSLNNEHGRLREIVDAGTA
                     AFFDVLDLRLSIDKCGLPRGGAVSFHGKVISSDGMECERIPEVGTIPIPVPDRRATMR
                     FWDV"
     gene            866..1885
                     /locus_tag="PKH10_07955"
     CDS             866..1885
                     /locus_tag="PKH10_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNT28096.1"
                     /translation="MQGMIRRDPVSHRWVIFAPEKAPILTHGSSEEETLLPAENPDSC
                     PFCPGNEATTGSEILRYNHFPHLNNGWSLRVFPARNPLLRVEEDARPQGKGIYDAMHR
                     VGAHEVVVETARHGGAIDMLSPEEFRDVFLAWRDRSADLQGDRRFKYLMIFKNRGKEA
                     GGTVNHHYSELVAFPFLPSFVESRFRHAADYFAFRRRCVVCDIVDQELADGSRLVEEN
                     EEFAAFCPYAGRFPFEVWIVPKRHLLYFSDTPHETLLALTRIYLAVLRRIRVALGAPA
                     YNVVVYNGPSNYDDFTKNPLQFFHWHIEIIPRICGMNALPLGGGGYINPTLPEDAAAF
                     LREQG"
     gene            complement(1892..2926)
                     /locus_tag="PKH10_07960"
     CDS             complement(1892..2926)
                     /locus_tag="PKH10_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28097.1"
                     /translation="MRKHFLFITLVALFLMVACGDDTKTTTDNNVTPDDEVADDVVVD
                     ETVTDDTTIDETVTDTVATDNAVTDETVTDETGDDILNDNEQPDEDMAPDPLVQHLVG
                     VWAQKVELFADGNVPVVNDVVLRTDKIVRIIIKEEGGKAVLADRPQTCYMETVIVEGN
                     SIAKTITTYFPEKFRNYFFYIPPEDLKDPKPALEYSGTPDNFSFKMNRWWEMRGAYFQ
                     GENGTTPFDIETEMITETDDPRIFDHDEDGKAGMTFEIKSSIASGLIWGVIKSYMEVT
                     STSAVNDRIEGSSLWQQYEMVIDVDNPLFAGDRTIQPHTEGNTMVMVRIDPADDCAAI
                     NEKKDTLFKK"
     gene            complement(2936..>4122)
                     /locus_tag="PKH10_07965"
     CDS             complement(2936..>4122)
                     /locus_tag="PKH10_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28098.1"
                     /translation="GSYETKSMMDHPDFLNFYTLLAGNMSWFDGWLAGALYVELPASQ
                     LTGQVPYYPDERDQFFGNSLHFELFGDRLESFHLVMALAGKVGDWLSLGAGATVGVQA
                     VADIGVYVADASSPEYLLFDPAIAVHALPLFAPHFGFVITPIDDLAITGTLHLPNLNF
                     DFNLDVDIQVRGFDSLYDDDPNNPGEKLKWLDSGLRTNYGNDPLRFSLGVMYSFPKVE
                     GWRISLIANALYTMWSQYRDRHAERPLDKWHDTVKPSFGVNVASEDREFGFDWTYHMT
                     PVPDQTGRSNYVDNDRIAFAIGYTEFFKFEKFTLAGGVSFQTQVLLKRTTIKTRTEEG
                     YASVTDAPYNSVVDEYPEMTDPEGEVYPGSVGFQTNNPGYPGYWTRGVLFGSGIFAKF
                     IF"
BASE COUNT          813 a         1278 c         1193 g          838 t
ORIGIN      
        1 ggacaacacg gcatgggaac atctgcgtcg gacgcgcctg ttcctcgacg aacacctgaa
       61 gacccacccg gacactccgc cggagacccg cgaggaggcg ctccgagcca tttaccgtgc
      121 cgagggaagc gactggttct ggtggtacgg cccgcagttc cagaccgaga acgactacct
      181 gttcgaccgc ctcttccgcc gccatctgcg ccgcgtctat gccgtgctcg gtgctccgca
      241 tccgacggcg ttggacctcc cgataaagcg gtcggccggg gtcaccgata accttaccct
      301 gccgataacg accatatcgc ctgagatcac ggggcggacc gacgattttt acggctgggc
      361 aggggctggc ctgttcgaca acacgcggcg gcccggcggt gtcatgtacc acggcgtccg
      421 cgtggtcgac cgcctttggt tcgggttcga ccgcgaccat ctctatttcc gagtggcgct
      481 cgccgcgcct cccgtcacgc aggaacacaa acgatcggcc atacgcttct atatcaccga
      541 cggcatgagt ctcgacctgc aactgccgat agcgcggttc gaaaaggcat tcttccccgt
      601 ttcgctgaac aacgaacacg gcaggttgag agagatcgtg gacgccggta ccgcagcgtt
      661 cttcgatgtc ctcgacctcc gcctttccat cgacaaatgc ggcctgccac gcggcggcgc
      721 cgtgtcgttc cacggcaagg tcatctcgtc ggacggtatg gaatgcgaac ggataccgga
      781 ggtcggcacc atcccgatac cagtgcccga tcggcgggcg accatgcgat tctgggatgt
      841 ctgaagaacg gaaaggaggc cacccatgca gggaatgata cgccgggacc ccgtttcgca
      901 ccgctgggtg atcttcgccc ccgaaaaagc ccccatcctg acgcacggat cctccgagga
      961 ggagaccctg ctccccgccg agaaccccga ctcctgcccc ttctgtcccg gcaacgaggc
     1021 gacgaccggc agtgagatcc tgcgctacaa ccacttccct cacctcaaca acggctggtc
     1081 gctccgcgtg ttcccggcgc gcaacccgct gctacgcgtc gaggaggacg cgcgaccgca
     1141 gggcaagggc atttatgacg ccatgcaccg cgtcggcgcc cacgaggtgg tggtcgagac
     1201 cgcccgccac ggcggggcga tagacatgct gtcaccagaa gagttccgcg acgtgttcct
     1261 cgcgtggcgc gaccgctcgg ccgatctgca gggcgaccgg cggttcaaat acctcatgat
     1321 cttcaagaac cgcggcaaag aggcgggcgg cacggtcaac caccactact ccgaactggt
     1381 cgccttcccg ttcctcccct cgttcgtcga gtcgagattc cggcacgctg ccgattattt
     1441 cgcgttccgg cgccggtgcg tcgtctgcga catcgttgat caggaactcg cggatggatc
     1501 ccgtctcgtc gaggagaacg aagaattcgc cgccttctgc ccctacgcgg ggcgcttccc
     1561 cttcgaggtg tggatcgtgc cgaaacggca tctgctctac ttcagcgaca ctccccacga
     1621 gacgcttttg gcgctcaccc gcatctatct ggcggtcctg cgccgtatcc gggtcgctct
     1681 cggggccccc gcctacaatg tcgtggtgta caacggaccg tcgaactacg acgatttcac
     1741 caagaacccg ctccagttct tccactggca catcgagata attcctcgga tatgcggcat
     1801 gaacgccctg ccgctcggcg gcggcggcta catcaatccg acccttccgg aggatgcggc
     1861 cgccttcttg agagaacagg gctgacggcc tctatttttt gaacagcgta tctttcttct
     1921 cgtttatcgc ggcgcaatcg tccgccggat cgatcctgac catgaccatc gtgttccctt
     1981 ctgtatgcgg ctggatggtc cgatcgcccg cgaagagcgg attgtcgaca tcgatgacca
     2041 tttcgtactg ctgccacagc gaggagccct ctatacgatc attcacggcg gaggtagagg
     2101 tcacctccat gtagctcttg atgactcccc agatgagtcc cgaagcaatg gatgatttta
     2161 tctcgaaggt cattcccgcc ttgccgtcct catcgtggtc gaagatgcgc gggtcatcgg
     2221 tctcggtgat catctcggtc tcgatatcga agggggtggt gccgttctcg ccttggaagt
     2281 aggcaccgcg catctcccac cagcggttca tcttgaagga gaagttgtcc ggggtaccgc
     2341 tgtattcgag cgccggtttc ggatctttga ggtcttcggg cggaatgtag aagaaatagt
     2401 tgcggaactt ctcggggaaa taggtggtga tggtctttgc tatggaattg ccctcaacaa
     2461 tgaccgtctc catgtaacag gtctgcggcc gatcggcgag gaccgccttg cctccctcct
     2521 ccttgatgat gatgcggacg atcttatccg tgcgcaggac cacatcattg accaccggca
     2581 cattgccgtc ggcgaagagt tcgaccttct gcgcccagac accgaccaga tgctgcacga
     2641 gcggatcggg ggccatgtcc tcatcgggtt gctcgttgtc attcaggata tcatcgccgg
     2701 tctcgtcggt cacggtctca tcagtgaccg cgttatcggt cgcgaccgta tcggtcacgg
     2761 tctcgtcgat cgtcgtgtcg tcggtcaccg tttcatcgac gaccacatca tcggccacct
     2821 catcgtccgg ggtcacattg ttgtcggtcg tcgtcttcgt atcatcacca caggcgacca
     2881 tcaggaacaa cgccacgagg gtgatgaaca ggaaatgttt tctcatctcg gcctcctaaa
     2941 aaatgaattt cgcgaatatg cctgaaccga acagaacgcc tcgggtccag tagcccggat
     3001 aaccggggtt gttggtctgg aacccgaccg aaccgggata cacctcccct tcgggatcgg
     3061 tcatctcggg gtattcgtcc acgacgctgt tatagggtgc gtcggtcacc gaggcgtaac
     3121 cttcctcggt acgggtcttg atcgtggtgc gtttgagcag gacctgtgtc tggaacgaca
     3181 ctccgcccgc cagcgtgaat ttctcgaact tgaagaactc ggtatagccg atcgcaaagg
     3241 cgatgcggtc gttatccaca tagttcgagc gaccggtctg atcgggcacc ggggtcatgt
     3301 ggtaggtcca gtcgaacccg aactcgcggt cctccgaggc gacattgacg ccgaacgacg
     3361 gttttacggt atcgtgccac ttatcgagcg gtcgttcagc atggcgatcg cggtactgcg
     3421 accacatggt atagagcgca ttcgcgatga gcgagatacg ccacccctcg accttgggga
     3481 acgaatacat gaccccgagc gagaatcgaa gcggatcgtt gccgtagttg gtccgcaacc
     3541 cggaatcgag ccatttgagt ttttcgcccg ggttattcgg gtcgtcgtcg taaagggaat
     3601 cgaacccgcg cacctgtatg tcgacatcaa ggttgaaatc aaagttgaga ttcggcagat
     3661 gcagcgtgcc ggttatcgcg agatcatcga taggtgtgat aacgaacccg aaatgaggag
     3721 cgaaaagcgg caaggcatgt accgctatcg ccggatcgaa cagcagatac tccgggctcg
     3781 atgcgtcggc gacatagacg ccgatatcgg cgacggcctg cacaccgacc gtggcaccgg
     3841 caccgagcga aagccaatcg cccaccttgc cggcgagcgc catgacgagg tggaaactct
     3901 cgagccggtc accgaacagt tcgaaatgga gtgagttgcc gaagaactgg tcgcgttcgt
     3961 cggggtaata gggcacctgt ccggtaagtt ggctcgccgg caattcgaca tagagcgcgc
     4021 ccgcaagcca gccgtcgaac cacgacatgt tgcccgccag cagggtgtaa aagttaagaa
     4081 aatcaggatg atccatcatg ctcttcgttt cataactacc ac
//
LOCUS       DAOQUE010000238         4113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_24357_length_4113_cov_4.185313, whole genome shotgun sequence.
ACCESSION   DAOQUE010000238 DAOQUE010000000
VERSION     DAOQUE010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24357_length_4113_cov_4.185313"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PKH10_07970"
     CDS             complement(<1..1062)
                     /locus_tag="PKH10_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNT28099.1"
                     /translation="MKCFFCQIPVPDNEPSCAACHRDLAKACPACALLYEREANFCMA
                     CGGKLSDRYAGAAKTVPPAPAKHAKSAPKRSLNERPQHITLTEIIIPDEPPPNGKDIE
                     IDLGSLEESLHRRLAAEAEQERHPVATPTRAPNETIPPIDTAPEYEEDTALTGELSPQ
                     ASEITFHEPERSLSPYLSHNTYPTSLQEISDAITAQIHDPVIPTLFTAIGKRITVSNG
                     GFFTLEAPAGTGKGHFTAAVEQFVAQNPLYDTNIVVSTVNPFDFDYTIFIHLVRSVLN
                     IKTVDQQQVRAKIDKYFSDILPAGKRESLTALLCLNFTPVQVKLPKSDLDYLLAFMCY
                     WSARNKPILWVVDNAGQVNL"
     gene            complement(1120..1806)
                     /locus_tag="PKH10_07975"
     CDS             complement(1120..1806)
                     /locus_tag="PKH10_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28100.1"
                     /translation="MASWHRRVGVRTALAIILTTITALTVFLYRESYGARASFCDDCA
                     AETTVTETGTLRYADGHTIELRNRYHLLQQVKRIDDRLRITGLITGEIDRRLSAFLSH
                     DRFDISIATDGIEDLCPQHLSPLYPASARRVLFLLPSGAVYAGKGWLGSFCDGRLSCR
                     YRTEYITADGFEYTVACAGTLPDRTELRIMLRAFFDLERGWFRSVGGTVRSARETLES
                     WWVIGEEMII"
     gene            complement(1832..3175)
                     /gene="dnaB"
                     /locus_tag="PKH10_07980"
     CDS             complement(1832..3175)
                     /gene="dnaB"
                     /locus_tag="PKH10_07980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNT28101.1"
                     /translation="MKQLITLPHNLEAESSLLGCLLLKGGLYGTVEEHGLIPADFWET
                     RHQNIFRAIQDVFRSGRTPDLVTVSDELTKRGELEFVGGIPFLSDLMEAAQLTAEAAL
                     KDHAGIVADKSLHRQLIKICEDNASLSYRQERDFDFIVNQTTNELIQLSIKRNARSVV
                     KLDKLIDEELGALDQRVKHGAILQGITSGYPQLDNLTTGFKPGEMVVVAGRPGMGKTS
                     FALNMAASMARQGATVLFFTIEMPSSQLVRRIISQECLINAKNFTTGKIEEDEYERLW
                     REVDTLSKLPFYIDESSYLTMADVRSKAKKIRVEAGKLDAVFIDYIQLMHDPSYDKTP
                     DKVREIEHISHNIKLFAKDIGIPVITLAQLSRKIEERKEGVPMLSDLRDSGAIEQDAD
                     VVIFIHRNIGKESQRDLTSILVKKNRNGQQGEVKLRFLPHYTKFAPIDETMEEEN"
     gene            complement(3172..3684)
                     /gene="rplI"
                     /locus_tag="PKH10_07985"
     CDS             complement(3172..3684)
                     /gene="rplI"
                     /locus_tag="PKH10_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNT28102.1"
                     /translation="MKIILTKDIDKLGNAGDVVRVKDGYGRNYLVPQGFALPANEKNI
                     KELEFNRRMIQKKIDGEYGKARELGEKIAAVDISIAKKVGEEGKLFGSVTTREIADAL
                     KEKGYEIDHRHIILDGPIKKSGVYEVEVKLFRELKGKVKLWVVALEEDAAPEAEPAPE
                     TATGEKTATE"
     gene            complement(3695..3919)
                     /gene="rpsR"
                     /locus_tag="PKH10_07990"
     CDS             complement(3695..3919)
                     /gene="rpsR"
                     /locus_tag="PKH10_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNT28103.1"
                     /translation="MVTYRRRKVCRFCANAEIPIDYKHPEVLLNYITERGKIIPRRIS
                     GICAKHQRDVAREIKKSRTIALLPYTVTGR"
BASE COUNT          665 a         1158 c         1361 g          929 t
ORIGIN      
        1 cagattgacc tgcccggcgt tatcgaccac ccacaggatc ggcttattgc gcgccgacca
       61 atagcacatg aaggcaagca ggtaatcgag atcgctcttg ggcagtttga cctgcaccgg
      121 ggtgaagttg aggcagagga gcgccgtcag cgactcccgt ttcccggcgg gaagtatatc
      181 ggagaaatat ttatctatct tggctcgcac ctgttgctgg tcgacggtct tgatgttgag
      241 caccgaccgg acgaggtgga tgaatatcgt ataatcgaaa tcgaacggat tgacggtgct
      301 gacgacgatg ttcgtatcgt acagcgggtt ctgggcgacg aactgttcca ccgcggcggt
      361 gaagtggccc ttgccggtgc cggcgggcgc ctcgagtgtg aaaaaaccgc cattcgatac
      421 ggtgatgcgt ttgcctatcg ccgtaaagag cgtggggatg accggatcat gtatctgcgc
      481 cgtgatggca tccgatatct cttggagcga agtggggtag gtattgtggg acagataggg
      541 gctcagcgag cgttccggtt cgtggaaggt tatctcgctc gcctgcgggg agagttcgcc
      601 ggtgagcgcc gtgtcctcct cgtactcggg agcggtatcg atcggcggta tggtctcgtt
      661 cggggcgcgg gtcggcgtcg cgacgggatg ccgttcctgc tccgcttcag ccgccagccg
      721 ccggtggagc gactcctcca gcgaccccag atctatctcg atatccttgc cgttcggcgg
      781 cggctcgtcg gggatgatga tctcggtcag cgtgatgtgc tgcggccgct cgttgagaga
      841 tcttttcggg gcgctcttgg cgtgtttcgc cggtgccggc ggaacggtct tggcggcgcc
      901 ggcgtagcgg tcggacagtt tgccgccgca ggccatgcag aaattcgcct cgcgctcata
      961 caggagcgcg caggcggggc aggccttcgc gagatcgcgg tggcaggcgg cgcaggacgg
     1021 ttcgttgtcc ggcaccggta tctggcagaa aaagcacttc atgcactaca gacaagtgtc
     1081 caatacaggg cgcgatggta tcgtttaacg ggtcgtttgt caaataatca tttcctcgcc
     1141 gatgacccac caactttcca gcgtttcccg tgcgctccgg acggtcccgc cgaccgagcg
     1201 gaaccacccg cgttcgaggt cgaaaaaggc gcggagcatg atgcgcagtt cggttcggtc
     1261 gggtagggtg ccggcgcagg cgacggtgta ttcgaacccg tccgcggtga tatattcggt
     1321 gcggtagcgg cacgaaaggc gaccgtcgca gaagctgccg agccacccct ttccggcgta
     1381 cacggcgccg gagggcagga ggaacagcac ccgtcgagcg cttgccggat acaacgggct
     1441 caggtgctga gggcacagat cctcgatgcc gtcggtggcg atggagatgt cgaaccggtc
     1501 gtgggaaagg aacgcgctca ggcgccggtc gatctcgccc gttatcaggc cggtgatgcg
     1561 caaccggtcg tcgatgcgtt ttacctgctg gagaagatgg taccggttgc gtagttcgat
     1621 ggtgtggccg tcggcatagc gcagggtgcc cgtttcggtc accgtggtct cggcggcgca
     1681 gtcgtcgcag aaagaggcgc gcgcgccgta cgattcgcga tacaggaaca cggtaagggc
     1741 cgtgatggtc gtcaggatga tcgcgagtgc ggtccggacg ccgacgcggc ggtgccacga
     1801 cgccatatcc tctcctccgg gggaacgagg atcagttctc ttcttccatc gtttcgtcta
     1861 tgggagcgaa cttggtgtag tggggcagga aacggagttt gacctctccc tgctgaccgt
     1921 tgcggttctt cttgacgagt atcgaggtga ggtcgcgttg cgattccttg ccgatgttgc
     1981 ggtgtatgaa gatgacgaca tcggcgtcct gctcgatcgc gccgctgtcg cgcaggtctg
     2041 agagcatcgg gaccccctcc ttgcgctcct ctatcttgcg gctcaactgg gcgagcgtga
     2101 tgaccgggat gccgatatcc ttggcgaaca gtttgatgtt gtgcgatatg tgttcgatct
     2161 cgcggacctt gtccggtgtc ttgtcgtacg aagggtcgtg catgagttgg atgtagtcga
     2221 tgaatacggc gtcgagtttc ccagcctcga cgcgtatctt cttggccttc gaacggacat
     2281 cggccatggt gagataggac gattcgtcga tgtagaaggg gagttttgaa agggtgtcaa
     2341 cctcgcgcca gagccgttcg tactcgtcct cctctatctt gccggtggtg aagttcttgg
     2401 cgttgatgag gcattcctgc gatatgatgc gccgcacgag ttgcgaggag ggcatctcga
     2461 tggtgaagaa gagcaccgtc gccccctgcc gggccatcga ggccgccatg ttgagggcga
     2521 aactggtctt gcccatgccg ggccgtcccg cgacgacgac catctcgccc ggcttgaacc
     2581 cggtggtgag gttatcgagt tgcgggtagc ccgaggtgat gccctgcagt atcgcgccat
     2641 gcttgacgcg ctggtcgagc gcgccgagtt cctcgtcgat gagtttgtcg agtttaacga
     2701 ccgaccgggc gttgcgcttg atgctcagtt ggatgagttc gttcgtggtc tggttgacga
     2761 tgaagtcgaa atcgcgctcc tgccggtagg aaagcgacgc gttgtcctcg cagatcttga
     2821 tgagttggcg gtggagcgat ttgtcggcga cgatgccggc gtggtccttg agcgccgcct
     2881 cggcggtgag ttgtgccgcc tccatcaggt cggaaaggaa cgggatgccg ccgacgaact
     2941 cgagttcacc ccgcttggtc agttcgtcgc tgacggtcac cagatcgggg gtgcgccccg
     3001 accggaacac atcctgaatg gcgcggaata tgttctggtg gcgcgtctcc cagaaatcgg
     3061 cggggatgag cccgtgctcc tccacggtgc cgtagagccc gcccttcagg agcaggcagc
     3121 cgagcaacga ggattcggcc tcgagattgt gggggagcgt gatgagttgc ttcattcggt
     3181 cgcggtcttt tccccggtcg cggtctcggg ggccggctcg gcctcgggcg ccgcgtcctc
     3241 ctccagagcg accacccaca gtttcacctt gcccttgagt tcgcggaaca gtttcacctc
     3301 gacctcgtac acgccgctct tctttatcgg gccatcgagg atgatgtggc ggtgatctat
     3361 ctcgtagccc ttctccttga gcgcgtcggc tatctcgcgg gtggtgaccg acccgaagag
     3421 tttgccctct tcgccgacct tcttggcgat gctgatgtcg accgcggcga tcttctcgcc
     3481 gagttcgcgc gccttgccgt actcgccgtc tatcttcttc tgtatcatgc ggcggttgaa
     3541 ctcgagttcc ttgatgttct tctcgttggc cggcagagcg aaaccctgcg ggacgagata
     3601 gttgcgaccg tacccgtcct tcacccgcac gacatcgccg gcgttgccga gtttgtcgat
     3661 gtccttggta agaatgatct tcatggcgtc ctccctagcg tccggtcacg gtgtagggca
     3721 acagggcgat ggtgcgcgac ttctttatct cgcgggccac atcgcgctgg tgtttcgcgc
     3781 agatgccgct gatgcggcgc gggatgatct tgccgcgctc ggtgatgtag ttcagcagga
     3841 cctcggggtg cttgtaatct atcggtatct ccgcgttcgc gcagaaacgg cagacctttc
     3901 ttctccggta agtgaccatg ggtttctcct tttatttaca gttcaatgtt ctcttcttcg
     3961 acatcctgcg acttcttcag actgctctga agtttcgcgt tgtattcgcg gttctcgtcg
     4021 accggcggct cggccggggc ggcgggcgcc gcaggccgtt cggtccgttc gggccgatcc
     4081 tcgtgggcgg gccgccattc gggcatgcgg tcg
//
LOCUS       DAOQUE010000239         4101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_24462_length_4101_cov_4.514582, whole genome shotgun sequence.
ACCESSION   DAOQUE010000239 DAOQUE010000000
VERSION     DAOQUE010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24462_length_4101_cov_4.514582"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2872
                     /locus_tag="PKH10_07995"
     CDS             <1..2872
                     /locus_tag="PKH10_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28104.1"
                     /translation="SSLMTELDQKKARKAEIDAKYEAKKAEQINKVEDQIGSVENKYA
                     EKLAKVEDQVQKEAEAERVRLQAELDKKAQEWSTKEEGFAKELAKLGQEESAIAQQFD
                     SQVDKLNQMLSDIDEKYAKLMQPYSTDSIIEKMNQEIAELDNKKVEEVNKLQEMLDAI
                     RSAKINETQSFQDRKAETKNKAELSKIEREHQANMRKIEMDEGALDRKLKFTEDSANR
                     SIQTKRSTYERQIAQNKEKLDKFDAEKAKETIDINKKIKVIGGEKAKALATHQKKVNE
                     AMRSQKVAAAKHDKEMKAGAVDINKKVAAVLGSVKQRQAAVLGEKAAELKALKEAKAG
                     IVQKIDDEAKAEKGKSTAEVAEIDKKMKDIRKEIKGWEKRSSKVDPAKMNEMRKPFQD
                     KIASLEAQKKALVAEKNQKMKGINDAVKQKIADIAKEKATVIGETKGFDALLKAEMKK
                     IAEKYAAQKSALRADEKKLAGEKAEKAAALTKEKAVLSKEKAALEKGFAKNIKKDIAA
                     LSAERAKEFKKLNGELSVVNKALAKHEEEKDMLLAQAVEAIDMAYDAKSTELDMKNVA
                     ENKRLQSENDKFTKTKKAEKAQAEKAYKTFLAEKERFRKNIDKSVADAQKERDRKLSD
                     RKKQRDNEGKKWEQEKENRKKAFEARTASTRQKIADLTKEVEKLSNDVAVINADWAKK
                     LDDTKVKNQAEGQKKEKAWTDRQQKETDLYEEGKRKITEKYEQMEIKEKNEREKQKQT
                     MMAQRDKLLAEKEKRKEKRQGILAKEKEAWEKKQAKSKDDEQKLRDEIERSKSKREED
                     ASQDKEDARKKKAAIDTEYQKRLDKIAEEELKVIQSRYKEAYKIESKRDLELTATTKN
                     IEAKLAEVLAKNGLGKLNENDLSGARDAFVQALYLNKNDKTAKEGLNSINSKAKAIYW
                     EAFGIKESDKTKAKKMLQQLLKSLLATDEYFLRSRALLEEIK"
     gene            2916..3734
                     /locus_tag="PKH10_08000"
     CDS             2916..3734
                     /locus_tag="PKH10_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28105.1"
                     /translation="MLPLLFVLCSFPVFAATSTASYTDLYRVFHMPLSEVPGWLKKNK
                     GDRLKDLFLARYHLPLTARGAMQRVPLDIARSYNEGYQYATLYIEAPKKETFFFLLSG
                     ILKGTIAVNGEQKGTLSPAHPFDHSTIELTLEPGVYSVLFAFEPLSCDGQFILLTDRA
                     ITYASRGFTRNFRSTLKVREHPCPKRELAEWLYERRIFPFSQDDDHTYAATRTVDDPE
                     ATVENDFCEKTPLLVLLRHAKDRKAREMLSGIGFDEKALEWWDLFLGKGKKEDP"
     gene            3731..>4101
                     /locus_tag="PKH10_08005"
     CDS             3731..>4101
                     /locus_tag="PKH10_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28106.1"
                     /translation="MSDRRWNIMTTVVAIAALVIAFIAIVLVHRVGPVADDVSVLYDR
                     LDTLETQIEELREQKVGEGAKIKDVFSVAAMEEQMRRLRAEVDALKKGGGAESAVAVM
                     QSRQEEMLKGTNKAYFNTWKGV"
BASE COUNT         1283 a          927 c         1207 g          684 t
ORIGIN      
        1 gtcttccctc atgaccgaac tcgatcagaa aaaagcgcgt aaggccgaga tcgatgccaa
       61 atacgaagcg aagaaggccg aacagatcaa caaggtagag gaccagatag gatccgtcga
      121 gaacaagtac gcagaaaagc tcgccaaggt cgaggatcag gtccagaaag aggccgaggc
      181 cgaacgggtg cgcttgcagg ccgaacttga caagaaggcc caagaatggt cgaccaagga
      241 agagggattc gccaaggaac ttgctaaact ggggcaggag gaatcggcga tagcgcaaca
      301 gttcgattca caggtcgaca agttgaatca gatgctttcg gacatcgacg agaagtatgc
      361 gaaactcatg caaccctaca gcaccgattc gatcatagag aagatgaatc aggagatcgc
      421 cgaactcgac aacaagaagg tcgaagaggt aaataagctg caggagatgc ttgatgccat
      481 cagaagcgcg aagataaacg aaacgcagtc gttccaggac aggaaggccg agacaaagaa
      541 taaggccgaa ctctccaaga tagagcgcga acatcaggcg aacatgagaa agatagaaat
      601 ggacgagggg gcgcttgacc gtaagttgaa attcaccgag gattcggcga atcgtagtat
      661 tcagaccaaa cgctccacct acgaacggca gatagcccag aataaggaga aactggacaa
      721 gttcgatgcc gaaaaggcga aggaaaccat agacataaat aaaaaaataa aggttatcgg
      781 tggagaaaag gcgaaggctc ttgctaccca ccagaaaaag gtcaacgagg cgatgcggtc
      841 gcagaaagta gcggcggcta agcatgacaa ggagatgaaa gcgggcgcgg tcgacatcaa
      901 caagaaggtg gcggcggtcc ttggatccgt caagcaacga caggcggcgg tcctcgggga
      961 aaaggcggcc gaattgaagg cgctgaaaga ggcgaaggcg ggcatagtgc agaagataga
     1021 tgacgaggca aaagcggaga agggtaagag cacggcagag gttgccgaga tcgacaagaa
     1081 gatgaaggat attcgcaagg agatcaaagg ctgggaaaaa aggagcagca aggtcgatcc
     1141 ggccaagatg aacgagatgc gcaaaccctt ccaggacaaa atagcctcgt tggaagcgca
     1201 aaagaaggcg cttgtcgccg aaaagaacca gaaaatgaaa ggcatcaatg atgccgtcaa
     1261 gcaaaagata gccgatatcg ccaaggaaaa ggcgacggtc ataggcgaga ccaagggttt
     1321 cgatgcgctt ctgaaggcgg agatgaagaa gatagcagaa aaatatgccg ctcagaaaag
     1381 tgcgttgcgc gccgatgaaa agaaactcgc tggcgagaaa gcggagaagg ctgcggccct
     1441 gacaaaggag aaggccgtgc tttcaaaaga gaaggctgcg ctcgaaaaag gattcgccaa
     1501 gaacataaag aaggatatcg cggcactttc ggccgaacga gcgaaggagt tcaaaaaact
     1561 gaacggagaa ttgtcggtcg ttaacaaggc gctggcaaag catgaagagg aaaaggacat
     1621 gctcctggcc caggctgtcg aggcgataga catggcctat gatgctaaat cgaccgagct
     1681 tgacatgaaa aatgtcgccg agaacaagcg cctgcaatcc gagaatgata agttcacaaa
     1741 gacgaagaag gccgaaaagg cccaggcgga aaaggcgtac aagaccttcc tcgccgaaaa
     1801 ggagcgtttc aggaagaata tcgacaaatc ggttgctgat gcacagaaag aacgcgaccg
     1861 gaagctctct gatcgcaaga agcaacggga caatgaaggc aagaagtggg aacaggaaaa
     1921 ggagaatcgc aagaaggcgt tcgaggcgcg caccgccagt acccgtcaga agatagctga
     1981 tctcaccaaa gaggtcgaga aactttctaa cgacgtcgcc gtgatcaacg ctgactgggc
     2041 gaagaaactc gacgatacca aggtcaagaa tcaggccgaa ggccagaaaa aggaaaaggc
     2101 gtggaccgat cgccaacaga aagagaccga cctttacgaa gagggtaagc gaaagatcac
     2161 cgaaaagtac gagcagatgg agataaagga aaagaacgag cgcgagaagc agaaacagac
     2221 gatgatggcc cagcgcgata aacttctcgc cgaaaaggag aagaggaaag agaaacggca
     2281 gggtatcctt gcgaaggaaa aggaggcttg ggaaaagaag caggcgaagt cgaaagatga
     2341 cgagcaaaaa ctccgcgacg agatagagcg gtcaaaatca aaacgcgagg aggacgcttc
     2401 ccaggacaag gaagatgcgc gtaagaagaa ggccgcgatc gacaccgagt accagaagag
     2461 actcgataag atagcggaag aagaactcaa ggtcatccaa tcgcgttaca aggaggccta
     2521 taagatcgag tccaagcgtg acctagagct taccgcaacc acaaagaaca tagaggcgaa
     2581 actcgcagag gtgctcgcga agaacgggct cgggaaactg aacgagaacg atctttccgg
     2641 tgcccgcgat gccttcgtgc aggccctata tctcaataag aacgacaaaa cggcgaaaga
     2701 gggtctgaac tctatcaact cgaaggctaa agccatctac tgggaggcct tcggtatcaa
     2761 agagtccgac aagaccaagg caaagaagat gttgcagcaa ttgctcaaga gtctgcttgc
     2821 gaccgacgaa tatttccttc gatcccgcgc gttgctcgaa gagatcaaat aaccatccgc
     2881 ggatgaaagg tcttataggg aggggagcac gcaccgtgct ccccttgctt tttgtcctct
     2941 gttcttttcc ggtctttgcc gcgacatcca ccgcgtcgta tactgatctg taccgcgttt
     3001 tccatatgcc gctctcggag gtccccggct ggttgaaaaa gaataagggc gatcgtctaa
     3061 aagatctttt tcttgcgcgt tatcatcttc ctttgaccgc gcgtggcgcc atgcaacgcg
     3121 tcccgttgga tatcgcgaga agttataacg aggggtacca atacgccact ctctatatcg
     3181 aagcgccgaa aaaagagacc ttttttttcc tgctctccgg aatactcaaa ggaacgatcg
     3241 ctgtgaacgg cgaacagaag ggcacgcttt cgccggcgca tccctttgac cattcgacaa
     3301 tagaattgac cctggaaccc ggtgtctatt ccgtgctttt tgcgtttgaa cctctttcgt
     3361 gcgacggaca attcattctc ttgacggatc gggccataac atacgcctct cggggtttta
     3421 ctaggaattt tcgctcaacc cttaaggtca gggaacatcc gtgcccgaaa cgggaacttg
     3481 ctgaatggct ctatgagagg aggatctttc ccttctcgca ggacgatgac catacttacg
     3541 cggcaacgcg caccgtggat gatcccgagg ccaccgtcga gaatgatttc tgtgaaaaga
     3601 cacccctgtt ggtccttttg cgacatgcga aagatagaaa ggcgcgagag atgctcagcg
     3661 gtataggttt cgatgaaaag gcgctcgaat ggtgggatct attcttgggc aaggggaaga
     3721 aggaggatcc atgagcgata gaaggtggaa cattatgacg accgttgttg cgatcgccgc
     3781 gttggtcatt gcattcatag ctatcgtcct ggtgcatcgg gtcgggcctg tcgccgacga
     3841 tgtgagcgtg ctttacgacc gtttggatac tcttgagacg cagatcgaag agttgcgcga
     3901 gcaaaaggtc ggggagggag caaagatcaa agatgtcttt tccgtcgcgg cgatggaaga
     3961 gcagatgcgc cggcttcgcg ccgaggtcga tgcgcttaaa aaaggagggg gcgccgagag
     4021 cgccgtggcc gtgatgcagt ctcggcagga agagatgctc aaggggacga acaaggccta
     4081 tttcaacaca tggaaaggag t
//
LOCUS       DAOQUE010000240         4074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_24703_length_4074_cov_4.112963, whole genome shotgun sequence.
ACCESSION   DAOQUE010000240 DAOQUE010000000
VERSION     DAOQUE010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24703_length_4074_cov_4.112963"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKH10_08010"
     CDS             <1..542
                     /locus_tag="PKH10_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT28107.1"
                     /translation="SLAEHFAVRSFTAPEVVTSTDGTAKYRFTLDDGAIIESVLIPEK
                     ERLTLCVSTQVGCPLGCLFCRTGSLGFTRDLTTREIIGQLWAVQRQVREAGKRVTNVV
                     FMGMGEPLLNLANTVDTIRIMLDDLGFNLSNRRVTVSTIGIPARIDELGGQVAVNLAL
                     SLHATTDVKRAALMPATKR"
     gene            663..884
                     /locus_tag="PKH10_08015"
     CDS             663..884
                     /locus_tag="PKH10_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28108.1"
                     /translation="MKIARSISAKVNLIPFNSFTGAPFHPSGTKATAAFQQVLWDHGM
                     MAIIRRSRGDEHLAACGQLGYVPLSEDDE"
     gene            1015..2403
                     /locus_tag="PKH10_08020"
     CDS             1015..2403
                     /locus_tag="PKH10_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28109.1"
                     /translation="MRIKSVLFFCLSLFLISCGSSYLFKIDTYAQQATLIGKTHKHKG
                     TDFCIVSDKTYFLARVPNSAGNTVEDETALYIVDNNGDDEDVILGEFYASRIAPSHDG
                     NLIVAGNIPSPNNSENDLSFLAKYDPAGYPIWGKTLKLGDIQENMMRVAKYAEGIVSL
                     AVGDDDSIYVVSQIKEGKNDGPPVYRFWDDFVVAKFDKNGELLWKKKEGSKNTHDQVT
                     AITIDNGVLYVAGITEGNLYAEHGGNSCETSMSNARYAAEVAVPCKEIFIASFSTQDG
                     SRLKQNQYTGEKIYNEVRSVAVGNGKIAVAGQTGPHAAVHYFSTETFEHLSTIFFDYT
                     SLRYEEMKKDDEEEKTHYSEVRSIAFDGNNFHVAGVVSEDQGIYYGNEYLATRSRDMW
                     DVSHVEQYQPRKMFVYKIDGNGGVVDSVFYKLWFTSPKSPKMIFHEGKRYILGDLFFW
                     GESKSNFIVIKK"
     gene            complement(2412..3839)
                     /locus_tag="PKH10_08025"
     CDS             complement(2412..3839)
                     /locus_tag="PKH10_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNT28110.1"
                     /translation="MRYALMLMVFLTFSPFLHAASIIWEVTVERDDPAIRDAFGALDV
                     RSAYRGPDGRWRYLVEATQEELSFLRSLPVRIAPRVPLAEEPETPVVEYRDADGVLAD
                     IRALEERYAGRAKVYTIGTSFEGRDILAVRISAAPSKNLPDKNEILIVGMHHAREWIA
                     VEVPLRLAEFVLEQYDHNPRIRAIVDDGELWIAPMLNEDGFRYTLSDDRNWRKNRRDN
                     GDGSFGVDPNRNYDASWGGKGASTKTSSDVYCGTEPFSEGESAAVRDLIDPANGMVDD
                     MLGFLDLHSYSQLILYPYGNTYEVSPREKELAAIADRMSAIIEGETGTYYEPGKSSSL
                     YIATGGSSDWFHLAYEFRNTFIVEMRPTGNVLNGFELDAAQIPATARENVAAALYFIE
                     ATAQGTDDIDTDVNGDGVIDYFAGCGKGRCEMIGLSEEKEENDDDLAADADASYGTDE
                     SMVLTDADAPEPRRDGSGGCSLILL"
     gene            complement(3859..>4074)
                     /locus_tag="PKH10_08030"
     CDS             complement(3859..>4074)
                     /locus_tag="PKH10_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNT28111.1"
                     /translation="MKLLAPGGVLASFSCSGAVTMDRFRQTIAFAAKDAGREVRILHH
                     LHQGSDHPVRVSVPETEYLKGVLGVVG"
BASE COUNT          887 a         1149 c         1153 g          885 t
ORIGIN      
        1 actcgctcgc ggagcatttc gcggtacgat cgttcaccgc acccgaggtc gtcacctcca
       61 ccgacggcac cgcgaaatac cgcttcacac tcgacgatgg cgcgatcatc gaatcggtgc
      121 tcattcccga aaaggagcga ctgacgctct gcgtctcgac gcaggtcggc tgtccgctcg
      181 gctgcctgtt ctgccgcacc ggctcactcg gcttcacccg tgacctgacg acccgcgaga
      241 tcatcggcca actctgggcc gtccagcgac aggtgcgcga agcgggcaag cgggtcacca
      301 atgtcgtctt catggggatg ggcgagccgc tcctcaacct cgcgaacacc gtcgacacca
      361 tccgcatcat gctcgacgac ctcggcttta acctctcgaa ccgccgcgtg accgtcagca
      421 ccatcggcat ccctgcgagg atcgacgaac tgggagggca ggtcgcggtc aatctcgcgc
      481 tgtcgctcca tgcgacgacc gatgtgaaac gggcggcgct gatgccggcg accaagcgct
      541 gaccgctgaa ggaactgctc gcggccatcc ggcgcttccc gctgccgccg cgcaaacgga
      601 tactcatcga atatgtcctg ctggcgggcg ttaacgactc ccccgccgag gcgcaggaac
      661 tggtgaagat agcccggagc atcagcgcaa aggtcaacct gataccgttc aactcgttca
      721 ccggcgcgcc gttccacccg tcgggcacca aggcgaccgc ggcgttccag caggtcctgt
      781 gggaccacgg catgatggcg atcatcagga ggtcccgcgg cgacgagcat ctcgccgcct
      841 gcggccaact cggctatgtg ccgctgtcag aggatgatga gtgaacagcc gccgcctatc
      901 tttgagtaaa aacttgcttt cctttttatt tttcttatgc ttttgtttaa cattgatttt
      961 gtttgttttt ttcacaggtt aagtcaggat gttaacaccg agggaggtta tctcatgaga
     1021 atcaaaagcg tactgttttt ttgtctgtct ttgttcctta tttcttgcgg gagttcttac
     1081 ctcttcaaga tagacaccta tgctcaacag gcaacattga tcggcaagac ccacaagcat
     1141 aaagggaccg atttttgtat agtttctgac aaaacctact tcctcgcacg tgttcctaac
     1201 agcgcaggaa ataccgtaga agacgaaacg gcactttata tcgttgataa taacggagat
     1261 gacgaagatg tcattttagg ggaattctac gcttcaagga tagccccctc gcatgacgga
     1321 aatcttattg tggctggcaa tatcccctct cccaataaca gcgagaacga cctctccttt
     1381 ttggccaagt atgatccggc gggctatcct atttggggga aaacactgaa actgggagat
     1441 attcaagaaa acatgatgcg tgtcgccaaa tacgctgaag ggatagtctc cttagcggtg
     1501 ggagatgacg attccattta tgtcgtatct cagatcaagg aagggaaaaa tgacgggccg
     1561 ccggtctatc ggttctggga cgatttcgtg gttgccaaat ttgataaaaa cggcgagttg
     1621 ttgtggaaga aaaaggaggg gtcaaagaat acgcatgatc aggtaaccgc catcaccata
     1681 gataacggtg ttctctatgt ggctggtatt acagaaggca atctgtatgc tgaacacggt
     1741 ggaaattctt gtgaaacatc gatgtcgaac gctcgttatg cggcggaagt agccgtcccc
     1801 tgtaaagaaa tttttattgc ctctttctcc acacaggacg gtagccggtt aaagcaaaat
     1861 caatacaccg gggaaaaaat ctacaacgag gttcggtccg tcgctgttgg caacggaaag
     1921 atagccgttg ccggccagac agggcctcac gcggctgtgc attatttcag cactgaaacc
     1981 ttcgagcatc tttcgaccat tttttttgat tatacctctc tccggtacga ggaaatgaaa
     2041 aaagatgatg aggaggaaaa aacacactat tcggaggtac ggagcatcgc tttcgacggc
     2101 aataattttc atgtcgccgg agttgtatcg gaagatcagg gaatctatta cgggaatgaa
     2161 tatttagcca ccagaagcag agatatgtgg gatgtctcgc atgttgaaca atatcaaccc
     2221 agaaagatgt ttgtttataa gatcgatggt aacggaggcg ttgttgacag tgttttctac
     2281 aaattgtggt ttacctcgcc caaatctcct aaaatgattt ttcatgaggg gaagagatac
     2341 atattgggcg acctcttttt ctggggagaa agcaaaagca atttcattgt tataaaaaaa
     2401 tagtgccgtt gtcagaggag gatgagtgaa cagccgcccg aaccgtcgcg gcgcggctcc
     2461 ggagcgtcgg catcggtgag gaccatcgac tcgtcggtgc cgtaggaagc gtcggcgtcg
     2521 gccgcgaggt cgtcgtcgtt ttcctccttc tcttccgaca gccctatcat ctcacagcgt
     2581 cctttgccac accccgcgaa gtagtcgatg acgccgtcgc cgttcacatc ggtgtctatg
     2641 tcgtcggtcc cctgcgcggt cgcctcgatg aagtagagcg ccgccgcgac attctcgcgg
     2701 gctgtcgccg gtatctgcgc cgcgtcgagt tcgaagccgt tcagcacatt cccggtcggc
     2761 cgcatctcga cgatgaaggt gttgcggaat tcgtaggcga gatggaacca gtcggacgac
     2821 ccgccggtgg cgatgtagag ggaactcgac ttgcccggtt cgtagtatgt gccggtctcg
     2881 ccctcgatga tggcggacat ccggtcggcg atcgcggcga gttccttttc gcgcggcgac
     2941 acctcgtagg tgttgccgta gggatagagg atgagttgcg aataactgtg tagatcgagg
     3001 aatccgagca tgtcgtccac catgccgttc gccggatcga tgaggtcgcg caccgccgcg
     3061 ctctccccct ctgagaacgg ctcggtcccg cagtagacat cggacgaggt cttcgtgctc
     3121 gcccccttgc cgccccacga ggcatcgtag ttgcggttcg gatcgacgcc gaacgatccg
     3181 tcgccgttgt cgcgccggtt cttgcgccag ttacggtcgt cactcaaagt atagcggaac
     3241 ccgtcctcgt tgagcatcgg cgctatccac agttcgccgt cgtcgacgat ggcgcggatg
     3301 cgcgggttgt ggtcgtactg ttcgagcacg aattcggcga gccgcagcgg cacctcgacc
     3361 gctatccatt cgcgggcgtg gtgcatgccc actatcaata tctcgttctt gtcgggcagg
     3421 ttcttcgacg gggcggcgct gatacggacg gcgagaatgt cgcgtccttc gaacgaggtc
     3481 ccgatggtat agaccttggc acgccccgcg taccgttcct ccagcgcgcg gatgtcggcg
     3541 agcacgccat cggcatcacg atattcgacc accggcgtct cgggctcctc ggcgagcgga
     3601 acacgcggcg cgatacggac cgggagtgag cgcaggaaag acagttcctc ctgcgtcgcc
     3661 tcgaccagat accgccaccg accgtcggga ccgcggtagg ccgaccggac atcgagcgcg
     3721 ccgaacgcgt cgcggatcgc cgggtcgtcc cgttcgaccg tcacctccca gatgatcgag
     3781 gcggcatgaa gaaacgggga aaaggtcagg aacaccataa gcattaaagc gtatcgcatg
     3841 actcctccgt tcatgacatc atccgacaac gccgagcact cctttgagat actcggtctc
     3901 ggggaccgac acccgcaccg ggtggtcgct cccctgatga agatggtgca ggatgcgcac
     3961 ctcgcgcccc gcatccttcg ccgcaaaggc gatggtctga cggaaccggt ccatcgtcac
     4021 ggcgcccgaa caggagaaac tggccagcac gccgccgggg gcgagcagtt tcat
//
LOCUS       DAOQUE010000241         4056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_24850_length_4056_cov_4.348163, whole genome shotgun sequence.
ACCESSION   DAOQUE010000241 DAOQUE010000000
VERSION     DAOQUE010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24850_length_4056_cov_4.348163"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2563)
                     /locus_tag="PKH10_08035"
     CDS             complement(<1..2563)
                     /locus_tag="PKH10_08035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNT28112.1"
                     /translation="MPKDLNEKPPEGRPVSPCDTLDVPEADDYMGFETLGSPGAMPVL
                     SSVSEDEEAITPETPGRYQTKQELGHGGIGRVLLVFDAHAGRDVALKEILPGILANKD
                     SDGRTPSSPSRVIHARFLREARVTGQLEHPSIVPVYEIGRHPDGSCYYTMRLVKGITL
                     SRAIAGCVDLEERLKLLTHFHDMCNALAYAHSRGVVHRDLKPDNVMIGEFGETVVLDW
                     GLAKVRGKKDHGARSLEKKILLLKESEESQTICGRVLGTPAYMPPEQAMGEIENIDER
                     SDIYSLGAVLYEILTGARPYEGGSPYEVVSKVVQGEPTAINLLEPSAPADLVAIAQKA
                     MSRKKERRYGQVVEMTRDIKNYLAGGRVSAYRYGPLELLGKFFRHHKALGVLSLVILL
                     LLVVGSGLLYSEYLRSQRSLLVSKALWYADRSRKSEQEKDYGASRVFASAALIHNPLV
                     KEGPFQIGKVEQAHPEVLPLYADINSRLYKSRLSMWAELERILPVNGPVWGAAFSPDG
                     RYLATVGPAKEGPSLVLWDTVAGTPHGSLSGFAGTAATVAFSPDGAMIAAGTQGGRIV
                     LWDAKTLQQKKGLSGHSGMVFAVAFSPDSQRLVSAGLDGTVRMWQVASGKESFAVKAH
                     PDGALCVAFSPDGKTVASGGWDKTVALWNAQNGRKLRVMTEHSDAVSAVAFATNGRAV
                     ASGGHDRSVMLWNLITDKRISLGTHRRQVTGLSFSRDGTLLASVGSDNATKIWDTRRE
                     ELALQMESHRKALNAVSFFGDSMLATGGNDKLVRLWRIHPGRSLVSYAGHTGRVTAVR
                     LIPNENLLLSVAEDGTIRFWDILNGLEILQLAVPPGIRALDVSRDGRYFAAAGMDT"
     gene            complement(2669..3508)
                     /gene="prmC"
                     /locus_tag="PKH10_08040"
     CDS             complement(2669..3508)
                     /gene="prmC"
                     /locus_tag="PKH10_08040"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNT28113.1"
                     /translation="MPQRVAEVIRAATERLNGATETPRLDAEILLAHVRGVDRSELLI
                     GYGGEVSDQDQARFDALVARRLTREPVAYIVGRKYFFEDEFFVDRRVLIPRPDTEHLV
                     EEALRLLRYRPEARILDLCCGSGCIGLTLARFTDAEVTFADLSPDALAVARLNTERLF
                     PGKGRRFHFIESDIFTSVAGTFDFIAINPPYLTVAETDALAGTPGAHEPRMAFDGGPD
                     GFAVSRRILADARRFLKPGGHLLMELGFLGAPMARTARTELRLVDIVRDYAGIERVAV
                     FAT"
     gene            complement(3513..>4056)
                     /locus_tag="PKH10_08045"
     CDS             complement(3513..>4056)
                     /locus_tag="PKH10_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010322670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNT28114.1"
                     /translation="GGVHRVQRVPVTEAQGRVHTSAITVAVLPEADEIEDYPIDEKDL
                     KIDTYRASGAGGQHVNKTDSAIRITHVPTGIVVAMQEERSQIQNRIRAMALLRSKLLQ
                     AEIEKRDRETAAQRKTLVGSGDRSEKIRTYNFPQNRCTDHRIGITLYRLGEIMEGSLD
                     MVIDPLITFQNAELLKSSLS"
BASE COUNT          777 a         1284 c         1215 g          780 t
ORIGIN      
        1 tggtgtccat gccggcggcg gcgaaatagc gaccgtcccg cgagacgtca agcgcacgga
       61 taccgggagg tacggcaagt tgcagtatct ccaatccgtt caggatatcc cagaaacgga
      121 tcgtcccatc ctcggccacc gacaggagaa gattctcgtt cggtatcagc cgcactgccg
      181 tgacccgtcc ggtgtgcccg gcgtaggaaa cgagcgacct gccgggatgt atccgccaga
      241 ggcggaccag tttatcatta ccgccggtgg cgagcatcga gtcgccgaag aacgaaacgg
      301 cgttcagcgc cttgcgatgg ctttccatct gaagcgccag ttcttcccga cgcgtatccc
      361 atatcttggt cgcgttatcg gacccgaccg aggcgagaag cgtgccgtcc cgcgagaacg
      421 aaagccccgt gacctgccgc cgatgggtac cgagcgagat ccttttgtcg gtgataaggt
      481 tccagagcat caccgaacgg tcgtgacctc ccgaggcaac ggcccggccg ttcgtcgcga
      541 aggcgaccgc cgagaccgcg tcgctgtgct cggtcatgac gcgcagtttt ctgccgttct
      601 gcgcgttcca gagcgctacc gttttgtccc agccgcccga cgccaccgtt ttcccgtcag
      661 gagaaaaggc gacgcacagg gcgccatccg ggtgcgcctt gaccgcgaac gattctttgc
      721 ccgaagcgac ctgccacatc cggacggtgc catccaaccc cgcggacacc aatcgttggg
      781 aatcgggcga gaaggctacg gcgaacacca tcccgctgtg gcccgacagt ccttttttct
      841 gctgaagcgt ctttgcatcc catagcacta tcctccctcc ctgtgtccca gcggctatca
      901 tcgcgccgtc aggcgagaac gcgacggtcg ccgccgtacc cgcaaagccc gaaagcgaac
      961 cgtgcggcgt tccggcaacg gtgtcccaga gcacgaggga tggcccctcc ttggcgggcc
     1021 cgaccgtcgc aaggtaccga ccgtcgggcg aaaaggcggc accccacacg gggccgttca
     1081 ccggtagtat cctctccagt tccgcccaca tactgagccg cgacttatac agacgcgaat
     1141 tgatgtcggc atacagcgga agcacttcgg gatgtgcctg ctcgaccttc ccgatctgga
     1201 acggaccttc cttcacgagc gggttatgga tcagagcggc cgacgcgaac acccgcgagg
     1261 caccatagtc cttttcctgt tcgctcttgc gcgatcggtc ggcataccaa agcgccttgc
     1321 ttacaagcag ggagcgttgc gacctcaggt attcactata caggagtccc gaaccgacga
     1381 ccagcaacag caggatgacg agtgacagga caccgagcgc cttatggtga cggaagaact
     1441 tgcccagcag ttcgagcggc ccgtatcgat aggcgctgac ccgaccgccc gcaagatagt
     1501 tcttgatgtc ccgcgtcatc tcgacgacct gcccgtaacg acgctctttt ttccggctca
     1561 tcgccttttg ggctatcgcc accagatcgg cgggggccga cggctccagc aggttgatgg
     1621 cggtcggttc cccctgaacg accttggaga cgacctcgta gggcgacccg ccctcatacg
     1681 gacgtgcacc ggtcagtatc tcgtacagca ccgcaccgag cgaatagatg tcggaccgct
     1741 cgtcgatatt ctctatctcc cccattgcct gttcgggcgg catataggcg ggagttccga
     1801 gcacccgacc gcagatggtc tggctttcct cgctttcctt caggagcagt atcttctttt
     1861 cgagacttcg cgcgccgtgg tcctttttgc cccgcacctt cgcaagaccc cagtcgagca
     1921 cgaccgtttc accgaactcg cctatcatca cattgtcagg cttgaggtcg cgatggacga
     1981 ccccccggct gtgggcatag gcgagcgcgt tgcacatatc atgaaagtgg gtcaggagtt
     2041 tcaaccgctc ctcgagatcc acgcaccccg caatggcacg ggacaatgtg atacccttga
     2101 cgagtcgcat agtgtagtaa caggaaccgt cggggtggcg gcctatctcg tagaccggca
     2161 cgatggaggg gtgttccaac tggccggtca cccgcgcttc gcgcaggaaa cgggcatgga
     2221 tgacgcgcga agggctgctg ggggtccgcc cgtcggaatc cttgttcgcc agtatgccgg
     2281 ggagtatctc tttcagcgcg acatcgcgac cggcatgggc gtcgaacacc agcaggacgc
     2341 gacctatgcc gccatgtccc aactcctgct tggtctgata gcgccccggc gtttcagggg
     2401 ttatcgcctc ctcgtcctcc gaaacggacg acaggaccgg catcgcgccg ggacttccca
     2461 gcgtctcgaa gcccatgtag tcatccgctt caggcacatc gagagtatcg cagggactta
     2521 ccgggcggcc ctccggcggt ttttcgttca ggtccttcgg catgagatac gcacccctct
     2581 ccggtgatca gatctccgcg tcttttccct acccttgtga acggggaatt ttaacgaaac
     2641 aatagggggg aagcaaattt attcacgact atgtcgcgaa caccgcgacc cgctctatcc
     2701 ccgcatagtc tcttacgatg tcgaccaacc gcagttcggt ccgcgcggtg cgcgccattg
     2761 gcgcgccgag aaagccgagt tccatcagca ggtggccgcc cggcttgaga aaacggcgtg
     2821 catcggcgag gatacggcgc gaaacagcaa agccgtcagg gccgccgtcg aacgccatac
     2881 ggggctcatg ggcgcccggt gttccggcaa gggcatcggt ctcggcaacg gtgaggtatg
     2941 gcggattgat cgcgatgaaa tcaaaggtcc ctgcgaccga tgtgaagata tcgctttcga
     3001 tgaagtggaa ccgccgcccc ttcccgggga agagtcgttc ggtgttgaga cgcgccaccg
     3061 cgagggcatc gggcgacaga tcggcgaaag tgacctccgc gtcggtgaac cgggcgagag
     3121 tcagaccgat acagccgctc ccacagcaga ggtcgagtat ccgggcctcg ggacggtaac
     3181 gcaggagacg caacgcctct tcgacgagat gttcggtatc ggggcgcggg atgaggacgc
     3241 gtcggtccac gaagaattca tcctcgaaaa aatacttgcg accgacgata taggcgaccg
     3301 gttcgcgagt gagtcgccgt gcgacaagcg catcgaacct tgcctgatcc tgatcggaca
     3361 cctccccgcc gtagccgatc aagagctcgc tccggtcgac cccgcggacg tgggcaagca
     3421 gtatctcggc atcgaggcgc ggcgtttcgg tcgccccgtt cagccgctcg gtggcggcgc
     3481 ggatgacctc tgccacgcgc tgcggcatcg cgttaactca gactgctttt aagcagttca
     3541 gcgttctgga aggtgatgag cggatcgatg accatatcga gacttccctc catgatctcg
     3601 ccgaggcgat agagggtgat cccgatacga tggtcggtac aacggttctg cggaaaattg
     3661 taggtccgta tcttttcgct acgatcgccc gaaccgacaa gcgttttgcg ctgggccgcc
     3721 gtttcgcggt cgcgtttctc tatctcggcc tgaagcagtt tcgagcgcaa aagcgccatc
     3781 gcccggatgc ggttctgtat ctgcgagcgt tcctcctgca tcgccacgac gatgccggtg
     3841 gggacatggg taatgcggat ggccgaatcg gtcttgttga catgctggcc gccggcgccg
     3901 ctcgcgcggt aggtgtcgat cttgagatcc ttctcgtcta tcggatagtc ttcgatctcg
     3961 tcggcctcgg gaaggacagc cacggtgata gccgaagtat ggacacgccc ctgcgcctcg
     4021 gtgaccggca cccgttggac acggtgaaca cctcct
//
LOCUS       DAOQUE010000242         4054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_24872_length_4054_cov_3.938985, whole genome shotgun sequence.
ACCESSION   DAOQUE010000242 DAOQUE010000000
VERSION     DAOQUE010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24872_length_4054_cov_3.938985"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..865)
                     /locus_tag="PKH10_08050"
     CDS             complement(29..865)
                     /locus_tag="PKH10_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28115.1"
                     /translation="MSRAALLSGILLFFRVAMATIRFGDQFPEELMSALATDPRTAAV
                     TEDMTLEVTPDGFILIISDEGIMRRLPVANTTADDVLNVIETMREEITVIRKAREQKS
                     EPPKAEKAPPEVEAPKKTEEPKKEEKPVVEWFAFADPDSRFYIGLTLSPEEAGAGGIE
                     FSGGVAFLRFGLNAKKGLNVKLAGAPSLEWEAYGVLAQIDVLRVTEVTFSTGLEVFLY
                     RLNNRIFERDQLFIGVAYKFLFMVVGVRAAVSPSDIVIQTEGKSYRTDQVRGVFFFSF
                     AF"
     gene            complement(862..1635)
                     /gene="deoC"
                     /locus_tag="PKH10_08055"
     CDS             complement(862..1635)
                     /gene="deoC"
                     /locus_tag="PKH10_08055"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNT28116.1"
                     /translation="MHDHDTILARAKELADLTGAGPERYAVIAANLVSVWEPPRLTAQ
                     SVPDLMDHTLLAFDATEKGIAQLCADAARHRFKAVCVNPVWVGTARVMRERLGARFAI
                     ASVVDFPLGASIDAARRAEAAAAITDGATELDLVIGIGLLRSGHLREVYEGIRCATGL
                     GGYLKAILETSALNEAEKLDAAILAVLAGADMLKTSTGVNGKATPDDVRLLRTVAGTA
                     IGVKAAGGIRDRATLERMVAAGADRVGSSASCGIMEEWG"
     gene            1774..2124
                     /locus_tag="PKH10_08060"
     CDS             1774..2124
                     /locus_tag="PKH10_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNT28117.1"
                     /translation="MRIEDFIRERERQNGVTLDFAGPVTKRFFSLDTQCYRDGALPVK
                     TKELIGLIASLVLRCDDCINYHLLRCRTEGVTDAELDEALSIGIIVGGSITIPHIRRA
                     VDGWKEMAEKDLSL"
     gene            2218..>4054
                     /locus_tag="PKH10_08065"
     CDS             2218..>4054
                     /locus_tag="PKH10_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28118.1"
                     /translation="MKKWVLSLIATAALTLFCAACDNNENEGLIDNGEATGDEDQDVG
                     ADKDVDIPKGDLDHDVDTDTENDVLPPTDNDIPSPWDDKDEDGDGIKNGFEGTGDTDS
                     DGTPDYLDDDSDGDGLPDSTEAPGGIPVDTDSDGTPDFQDTDSDNDGIPDGVEGTGDA
                     DSDGTPNYRDKDSDDDGLLDSIEAGGDPTIPLDTDSDGTPDYLDPDCDKDGIPDGVEG
                     LKDADLDGIPNYLDDDSDGDGVIDSEECPSLPCFDSDGDDLPDFKDKDSDNDGLSDAS
                     EKDCPILGKNSRFFADTDEDGYSDLAEEAVGSDLCDAAQGVKDIVEFYFVLPYQDPEK
                     TDVLTFAPTVQKADIHFNVDTTGSMSGEIDNLKSGLSTIMTEAKNRVADSAFGVSHFE
                     DFPACDFGGSSDQPWELLQSPTTDAGTAQAGVDKLTLGSGNDFPESGYESLYQLATGA
                     GGTFNLSGDDGCGGSCSASHTIPVASCGGLLGGACFRLGSIPIAIHVTDATSHVPADY
                     GLTYHSRDEAVTALNALGVRTITVQSGSDGDTTTQLNDISNSTGARVPVCAFKTGESS
                     WRCGAEQCCTLNGGATAPSGGVCTLRYQIPEDGTSLGSSVVDGVDA"
BASE COUNT          782 a         1383 c         1227 g          662 t
ORIGIN      
        1 gagccggcgc cggatgaggg aagaacggtc agaacgcaaa actgaagaag aacaccccgc
       61 gcacctgatc ggtgcggtaa cttttccctt cggtctgtat gacgatgtcg ctcggcgaga
      121 ccgcggcgcg gacaccgacc accatgaaca ggaacttgta ggcgacgccg atgaacaact
      181 ggtcgcgctc gaagatacgg ttgttgagcc ggtacaggaa cacctccagc ccggtcgaga
      241 aggtcacctc ggtgacccgc aggacatcga tctgcgcgag caccccgtat gcctcccatt
      301 cgagactcgg cgctcccgcc agtttgacat tgagtccttt cttcgcgttg agaccgaacc
      361 gcaggaacgc gaccccgccc gagaactcga taccgcccgc ccccgcctcc tcgggcgaca
      421 gagtgagccc gatgtagaac cggctgtcgg ggtcggcgaa ggcgaaccat tcgacgaccg
      481 gcttctcctc ctttttcggt tcttcggtct tttttggcgc ctcgacctcc gggggggctt
      541 tctcggcctt gggcggctcg cttttctgct cgcgagcctt ccggatgacc gttatctctt
      601 cgcgcatcgt ctcgatgaca ttgagcacat cgtcggcggt tgtgttcgcc accggcagac
      661 gacgcatgat accttcgtca ctgatgatga gtatgaaccc gtcgggtgtc acctcaagcg
      721 tcatgtcctc ggtgaccgcc gccgtgcgcg gatcggtcgc gagcgcgctc atgagttctt
      781 cggggaactg gtcaccgaac cggatcgtcg ccatggccac gcggaagaag aggaggatgc
      841 cggagaggag cgcggctcgg ctcatcccca ctcctccatg atgccgcacg aggcgctcga
      901 cccgacacga tcggcacccg ccgcgaccat ccgttcgagc gtcgcacggt cacggatgcc
      961 gccggccgcc ttgacgccga tcgccgtacc cgcgacggtc cgcagaagcc gcacatcgtc
     1021 gggcgtggcc ttgccgttca cgccggtcga tgtcttgagc atgtcggccc cggcgagcac
     1081 cgccagtatc gccgcgtcga gtttttccgc ttcgttgagc gccgaggtct cgagtatcgc
     1141 cttgagatac ccaccgagcc cggtcgcgca ccgtatccct tcgtagacct cgcgcagatg
     1201 gccggacctg agcaggccga tgccgatgac cagatcgagt tcggtcgccc cgtcggtgat
     1261 ggccgctgcc gcctcggccc gccgcgccgc gtcgatggat gcgccgagcg ggaaatcgac
     1321 gaccgacgcg atggcgaacc gcgccccgag ccgttcccgc atcacccgcg ccgtccccac
     1381 ccagacgggg ttcacgcaga ccgccttgaa acggtggcgc gccgcatcgg cgcagagttg
     1441 cgctatcccc ttttcggtcg cgtcgaaggc gaggagcgtg tgatccatga ggtcgggaac
     1501 gctctgcgcg gtgaggcgcg gcggctccca gaccgacacg aggttcgccg cgatgaccgc
     1561 gtaccgttcc gggcccgcgc cggtcaggtc ggcaagttct ttcgcccgcg ccagtatcgt
     1621 gtcgtgatcg tgcatgccgc ccaccctcac aagggaccgt ccggtggcga ttatagccgc
     1681 ccgttcggac aagaaaagaa aatattgttg catccgcgga cctcccgggg taccatgcgc
     1741 cccggcagta tccaccggaa cgggagtgtc gccatgcgca tcgaggactt catcagggaa
     1801 cgggagcgcc agaacggggt cacgctcgat ttcgcgggcc ccgtcaccaa acggttcttc
     1861 tcgctcgata cccagtgtta ccgcgacggc gctctgccgg tgaagacaaa ggaactcatc
     1921 gggcttatcg cctcgctcgt cctgcgatgc gacgactgca tcaactacca cctcctgcgg
     1981 tgccgcaccg aaggggtgac cgatgccgaa ctcgacgagg cgctttcgat agggatcatc
     2041 gtcgggggca gcatcaccat cccgcatatc cggcgggcgg tcgacggctg gaaggagatg
     2101 gcggagaaag acctctcgct ctgacccgcc gctatatttt tcgtcccctc ttgccttccg
     2161 gacccttttc gatacaatcc tccccatgac aacggatgac atcgtgcgga gcgaaccatg
     2221 aagaaatggg tgctttccct catcgcgacg gcggccctga cgcttttctg cgccgcctgc
     2281 gacaacaacg agaacgaagg cctgatcgac aacggcgagg cgaccggcga cgaggatcag
     2341 gatgtcggcg ctgacaagga tgtcgacata ccgaaagggg atctcgacca tgatgtcgac
     2401 accgacaccg aaaatgatgt cctccccccg accgacaacg acatacccag tccgtgggac
     2461 gacaaggacg aggatggcga cgggataaag aacggcttcg agggaacggg cgacaccgac
     2521 agcgacggca cccccgacta tcttgatgac gacagcgacg gggacggtct ccccgacagc
     2581 accgaggcgc cgggcggcat accggtcgat accgacagtg acggcacccc cgatttccag
     2641 gacaccgatt cggataacga cggcatcccc gacggcgtcg aggggaccgg cgacgccgac
     2701 agcgacggta ccccgaacta ccgggacaag gacagcgacg acgacggcct gctcgacagc
     2761 atcgaggcgg gcggcgaccc gaccatcccc ctcgacaccg acagtgacgg tacccccgac
     2821 tatcttgacc ccgactgcga caaggatggc atccccgacg gcgtcgaagg gctcaaggat
     2881 gccgatctcg acggcatacc gaactatctc gacgacgaca gcgacggtga cggcgtaatc
     2941 gacagcgagg aatgcccctc tctcccctgc tttgacagcg acggcgacga ccttcccgac
     3001 ttcaaggaca aggattcgga caacgacggg ctttccgacg cttcggaaaa ggactgtccg
     3061 atcctcggca agaactcgcg attcttcgcc gacaccgacg aggacgggta cagcgacctc
     3121 gccgaggagg cggtcggctc cgatctctgc gatgcggcac agggggtcaa ggacatcgtg
     3181 gaattctact tcgtcctccc ctaccaggac ccggaaaaga ccgatgtact caccttcgcc
     3241 ccgacggtcc agaaagccga catccacttc aatgtcgaca ccaccggctc gatgagcggc
     3301 gagatagaca acctcaaatc gggtctttcg accatcatga ccgaggcaaa gaaccgcgtc
     3361 gccgacagcg cgttcggcgt gtcccatttc gaggatttcc ctgcctgcga cttcgggggt
     3421 agcagcgacc agccatggga actcctccaa agccccacca ccgacgccgg cacggcgcag
     3481 gcgggggtcg acaaactcac gctcggcagc ggcaatgatt tccccgagtc ggggtacgaa
     3541 tcactctatc aactcgccac cggcgccggc ggcacattca acctttccgg cgacgacggc
     3601 tgcggaggca gttgtagcgc gagtcacacc ataccggtcg ccagttgcgg cggccttctc
     3661 ggcggcgcct gcttccgtct cggctccatc cccatcgcca tccatgtgac cgacgccacg
     3721 agccatgtcc ccgccgacta cggactcacc taccactcgc gcgacgaggc ggtgaccgcc
     3781 ctcaacgcgc tcggcgtccg caccatcacc gtgcagtcgg gctccgacgg cgatacgacc
     3841 acacaactga acgacatctc gaactcgacc ggcgcacggg tgccggtctg cgccttcaag
     3901 acgggcgaat cgtcatggcg ctgcggcgcg gagcagtgct gcacgctgaa cggcggcgcg
     3961 accgcgccgt cgggcggcgt ctgcaccctg cgctaccaga tacccgagga tggcaccagc
     4021 ctcggttctt cggtggtcga cggggtcgac gcca
//
LOCUS       DAOQUE010000243         4048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_24935_length_4048_cov_3.432256, whole genome shotgun sequence.
ACCESSION   DAOQUE010000243 DAOQUE010000000
VERSION     DAOQUE010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24935_length_4048_cov_3.432256"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="PKH10_08070"
     CDS             <1..1793
                     /locus_tag="PKH10_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT28119.1"
                     /translation="PPGPAGARRCAEPLADAPEKERGGRMTTTRRLLGYLWREKRLIG
                     GGILFTALMGLVEVGTGTILKMLTDGIEEISRFFKHGGEGIIEIPLKVKIPFPGSKGR
                     FKLLDTVISGADEVYRAMLYLALAVIILYLLKAVFEYTREICMNSAIQRILRRFRSEV
                     FAKLLGLPLSFHDRSRSGDLVSRVTYDVTTLERVLELFIEIARTAVYMLVFLPVMFVI
                     NAKITLFTMLFFPLSWLLITQFSRRIRRVGRDISETVGDYTAVMQDRTRTLRTIKGLS
                     AEATERSLFGALVERNYRLFVKMILLKYLMKPSNELIGMIGVSFVFIYFAGRLVSGDT
                     TLGNVVLYLYLMQQAYKPVKKVAAAFGELYNTLVCTGRIFSLFDESDEKQGGTAAIPA
                     DGALEIERLSFRYEGMPALFVGLSCRFPQGVTALTGSSGSGKTTLCALIQRFYEPAVG
                     TVTYGGTPLADMPLADWRRLVAYAGPDSALFAGTVAENLKYGNLTADEATLAAVTAVL
                     DIADDMLNAPAGEGGRLLAQGQREKAVLARAILRRPRILLIDELCDGMDDRGLDALFG
                     ILRDTPLVIIVSKSRPVLDRAAHLIRLNPS"
     gene            complement(1780..>4048)
                     /locus_tag="PKH10_08075"
     CDS             complement(1780..>4048)
                     /locus_tag="PKH10_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNT28120.1"
                     /translation="ENTFTMGEIVVTGERIANIEQAGTTTVITGEQLEERGVKTLDEA
                     LELVPGVQTQRHTKGFARIRLRGYEQDKVMILVDGVPMNDVYSTDIDLSSIPVRNIAK
                     LVVNRGVSSVLYGTDGAVGTVNVVTRKPDKPFVDAKIEYGVPDSTSISVAHGNAIDAF
                     YYWITGTYDEYGGFTPSATLTPEKRLQWFNKLIRYDRFGLTYDEVTIPAKEQYIFDDG
                     KWDHEHHRKGTFGLKGGWRITPDIETGLSSSLFIYSGRTNTYQSTCSSDYNVAKEKWT
                     VNSRPWFTDDITHLKDFALRNRAFVYPFLYRVTVSPYLDARWGDLSLKANLWYFRNHA
                     EQEGYATTDHRYPKDLTLLFTDKDDPTLYEPFRDIKDYGSLGLRLLPTYRFTKDYRMN
                     FAIHYRYDTFAADEGAVSATLSPTITATAGGTDPYPNTRIGAHTFYLAGEHELRAGDL
                     RLTAGLSYDMQAIDHFRVREGKEYGAAYIVKPDPIILGTRDAFNPAIGFTYDAVKDLL
                     LLRGAASVRARFPNLGEYAKVASAEFDKGLKPERAYNANAGVEFFFLDRKLSIRSDYF
                     ISLLDDRIAKISRDDPPVNIEKMVSQGIDTVITADFKHLAGMVDLGGSLTHSFVHARN
                     YDHSPEEQVNKGKYIEFTPSHQVLAELRIAVNTPHDIFRRTALSIWGSWSYGELIYTM
                     KEAPEEYAPFDTRYWEASTLHNPLFLNARISQELWEYFEFSFTAKNLLDDYASHPFNP
                     GPGRSFYLEAAFSWD"
BASE COUNT          719 a         1162 c         1331 g          836 t
ORIGIN      
        1 ggccgcccgg tcctgccggt gctcgccgtt gcgccgaacc gctggcagac gctcccgaaa
       61 aagaaagggg aggaaggatg accaccacgc ggcggctact gggttatctc tggcgcgaaa
      121 aacggctcat cggcggcggc atcctcttca cggcgctgat ggggctggtc gaggtcggga
      181 ccggcacgat actcaagatg ctcaccgacg gcatcgagga gatcagccgc tttttcaagc
      241 acggcggcga agggatcatc gagataccgc tcaaggtgaa gataccgttc cccggcagca
      301 aggggcggtt caaactgctc gataccgtca tatcgggggc cgacgaggtc taccgcgcga
      361 tgctctacct cgcgctggcg gtcattatcc tgtaccttct caaggcggtg ttcgaataca
      421 cccgcgagat ctgcatgaat tcggcgatac agcggatcct gcgccgattc cgctccgagg
      481 tgttcgccaa actgctcggg ctgccgctct cgttccacga ccggtcgcgc agcggcgacc
      541 tcgtttcacg ggtcacctac gatgtgacga cgctggaacg ggtgttggaa ctcttcatcg
      601 agatagcgcg cacggcggtc tatatgctcg tcttcctgcc ggtgatgttc gtcatcaacg
      661 cgaagataac gctcttcacg atgctcttct tcccgctgtc gtggctcctc atcacgcagt
      721 tctcgcgcag gatacggcgc gtcggccgcg acatcagcga aacggtgggc gactacacgg
      781 cggtcatgca ggaccggacc cgcaccctgc ggaccatcaa gggactgtcg gccgaggcga
      841 cggagcggag cctgttcggc gcactcgtcg agcggaacta ccgtctcttc gtgaagatga
      901 tactcctcaa gtacctcatg aagccgtcga acgaactgat cgggatgatc ggcgtttcgt
      961 tcgtcttcat ctacttcgcg gggcggctcg tgtcgggcga caccacgctc ggcaatgtcg
     1021 ttctttacct ctatctcatg cagcaggcct acaaaccggt caagaaggtg gcggccgcgt
     1081 tcggtgaact ctacaatacg ctcgtctgca ccggccggat attcagcctt ttcgacgaaa
     1141 gcgacgagaa gcagggcggc accgccgcca taccggccga cggagcgctg gagatcgaga
     1201 ggttgtcgtt ccgctacgag gggatgccgg cgctcttcgt tggcctgtcg tgccgtttcc
     1261 cgcagggcgt tacggcgctc accgggtcgt cggggtcggg gaagacgacg ctctgcgccc
     1321 tcattcagcg cttctatgaa ccggcggtcg ggacggtgac ctacggcggg acgccgcttg
     1381 ccgatatgcc gctcgccgac tggcgacggc tggtcgccta tgccggaccc gacagcgccc
     1441 tttttgccgg gacggtcgcc gagaacctga agtacggaaa tcttacggcc gatgaggcga
     1501 cgcttgccgc cgtgaccgcc gtgctcgaca tcgccgacga tatgctgaat gccccggcgg
     1561 gcgaaggggg acggttattg gcgcagggtc agcgcgaaaa ggcggtgctg gcgcgggcga
     1621 tcctacggcg gccgagaata ctgctcatcg acgaactctg cgacggaatg gacgaccgcg
     1681 ggctcgatgc actcttcggc atcctgcgcg acacccccct cgtcatcatc gtctcgaaaa
     1741 gccgcccggt gctcgaccgc gccgctcacc ttatcagact taatcccagc taaacgccgc
     1801 ttcgaggtag aaactgcgcc ccggacccgg gttgaagggg tgggatgcgt agtcgtcgag
     1861 gaggttcttc gccgtgaaag agaattcgaa atattcccag agttcctgcg atatccgcgc
     1921 attgagaaag agcgggttgt ggagcgtcga tgcctcccag tacctcgtgt cgaacggggc
     1981 gtattcctcg ggggcctcct tcatggtgta gatgagttcg ccgtaactcc aactgcccca
     2041 gatagacagc gcggtgcggc ggaagatgtc gtgcggggtg ttgaccgcga tccgcagttc
     2101 ggcgagcacc tgatgcgacg gggtgaactc gatgtacttc cctttgttga cctgttcttc
     2161 gggggaatgg tcgtagttgc gggcatggac gaacgagtgg gtgagcgaac cgcccagatc
     2221 gaccatcccg gccagatgct tgaaatcggc ggtgatgacg gtgtcgatcc cctgcgagac
     2281 cattttctcg atgttcaccg gcgggtcgtc gcgacttatc ttggcgatac ggtcgtccag
     2341 cagggatatg aaataatcgc tacggatgct gagtttccgg tcgaggaaga agaattccac
     2401 cccggcgttg gcgttgtagg cccgttccgg cttgagccct ttgtcgaatt ccgccgaggc
     2461 gaccttcgca tactcgccga ggttggggaa tcgcgcccgg accgacgcgg cgccccgcag
     2521 gagcaggaga tccttcaccg cgtcgtaggt gaacccgatg gccgggttga atgcgtcgcg
     2581 ggtgccgagg atgatcggat cgggcttgac gatgtaggcg gcgccgtact ctttgccttc
     2641 acggacgcgg aaatggtcga tcgcctgcat gtcgtaggaa agaccggcgg tcaggcgcag
     2701 atcgcccgct cgcagttcgt gttcgccggc gaggtagaag gtatgcgcgc cgatccgggt
     2761 gttcgggtag gggtctgtgc cgccggcggt cgccgttatt gtcggggaaa gggtcgccga
     2821 tactgctcct tcgtccgcgg cgaaggtgtc gtagcggtag tggatcgcga agttcatccg
     2881 gtaatccttg gtgaaccgat aggtgggaag gagccggagt ccgaggcttc cgtaatcctt
     2941 tatgtcgcgg aagggttcat agagcgtcgg gtcgtccttg tcggtgaaga gtagggtgag
     3001 gtccttggga tagcggtggt cagtggtggc gtacccctcc tgttcggcgt ggttgcggaa
     3061 ataccagagg ttcgccttga gcgagagatc gccccagcgg gcgtcgaggt acggcgagac
     3121 cgtcacgcgg tagaggaagg gatagacgaa agcgcggttc cgcagtgcga aatccttgag
     3181 gtgcgtgatg tcgtcggtga accacggacg gctattgacg gtccatttct ccttggcgac
     3241 attgtagtcg ctcgaacagg tcgactggta ggtgttggtc cgccccgaat agatgaacag
     3301 actcgacgaa agccccgttt cgatatcggg cgttatccgc cagccaccct tgagtccgaa
     3361 ggtccccttg cggtggtgtt cgtgatccca cttcccgtcg tcgaagatat actgctcctt
     3421 cgccgggatg gtcacttcat cgtaggtcag gccgaagcgg tcgtagcgga tgagtttgtt
     3481 gaaccactgg agccgtttct cgggggtgag cgtcgccgag ggggtgaagc cgccgtactc
     3541 gtcgtaggtg ccggtgatcc agtagtaaaa agcgtcgatg gcgttgccgt gagcgaccga
     3601 tatcgatgtg ctgtcgggga cgccgtactc gatcttcgcg tcgacgaagg gcttgtccgg
     3661 tttccgggtg accacattga ccgtgccgac ggcgccgtcg gtcccgtaga gcaccgagga
     3721 gacgccgcgg ttgacgacaa gtttcgcgat gttccggacc ggtatcgagg aaaggtcgat
     3781 atcggtcgag tagacatcgt tcatcgggac cccgtcgacg aggatcatca ccttgtcctg
     3841 ttcgtagccc cggagccgga tgcgggcaaa acctttggtg tggcgttgtg tctgaacgcc
     3901 gggtaccagt tccagcgcct cgtcgagcgt tttgacgccg cgttcttcga gttgttcgcc
     3961 cgtgatgacg gtcgtcgttc ccgcctgttc tatgttggct atccgttcgc cggtgacgac
     4021 gatctcgccc atggtgaagg tgttctca
//
LOCUS       DAOQUE010000244         4030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_25090_length_4030_cov_3.707421, whole genome shotgun sequence.
ACCESSION   DAOQUE010000244 DAOQUE010000000
VERSION     DAOQUE010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25090_length_4030_cov_3.707421"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="PKH10_08080"
     CDS             <1..1882
                     /locus_tag="PKH10_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNT28121.1"
                     /translation="DDKLYLPVYNMHLLYKYRFEEGYFPRCSSLRSSVWETTKEKLSR
                     EIEQVAEKILQLYAERAVEQGRPLPTSGEMVERFAAEFPYRETMDQSRSISELEQDLS
                     SGRIMDRLLCGDVGFGKTEVAMRGCMVTAAAGRQAAVLVPTTVLGFQHWQTFTERFRN
                     FPVRVELLSRFQSAAEQKKILVGLKEGSVDVVIGTHRLLSKDVEFRDLGFLVVDEEHR
                     FGVGQKERIKEMKKGVATLSMTATPIPRTLQLSLLGVRDISFIRTPPRERKPVRTHVV
                     EYSEEIVREAILREMERDGQIYFLHNRIESLSTMKRLLEKLVPDLKIAVAHGRMEEDQ
                     LEQVMLDFTARRFNLLLATSLIESGIDIPLVNTIVINRADTFGLAQLYQLRGRVGRWN
                     REAFAYLMVPSFKTLTKDAYDRLSVIKRFDRLGSGYDVAMEDLNIRGAGNILGLSQTG
                     KLKGVGYDLYLEMLRRRIEFLRTGQTGETRDIEVKTDLPAFIPETYIEDAETRVSFYR
                     KLADTERPEELGWVRHLLAEMFGPVPPEVENLFTLNEMRTLARKARVVKMDLSEGRMT
                     LTFSATATPKSMDDLFEAVDILRGKFVPPSGVQVPASTPDEWRKALERFGKVFGGTPT
                     YR"
     gene            complement(1891..2979)
                     /locus_tag="PKH10_08085"
     CDS             complement(1891..2979)
                     /locus_tag="PKH10_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28122.1"
                     /translation="MVEIPKFSEYYPSIESMNAQQRIFYKDLETKLNRGEFQDVDGNI
                     SYLFVYVYTLLAKWRNAGFESLHEFLIYLSELYKNEQKFSNYCLFWAHDCLLGQQQYE
                     EFLEKTEPKELFGTSTHQSNLRLNIQKKLNLEANPIDILLMVGGRKSKILSNNQALYK
                     EKIIEIFDQFANPHGGWFSIFDKWSDTSKLYSHSLFNGVPIMESPKLTFGIAAFYSMN
                     GNLEEIKKLSKEAENRVREELGIPQVGQGWVSETELFRKLESSFTQTQVLQHGQPPWL
                     GKQHFDIWFPNWKIAVEYHGQQHYEPVEFFGGVDAFNKNRERDERKANLAKRHGVKMF
                     VVNANDDQAELVKSIIGHIKNRKVLPPR"
     gene            complement(2981..3637)
                     /locus_tag="PKH10_08090"
     CDS             complement(2981..3637)
                     /locus_tag="PKH10_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28123.1"
                     /translation="MSGLAETISCSKKPSIFLDYSVIDHLFRIQEGSYNGPHSSALQS
                     LRYLAEKRGIDVWISEITPVEMLLGTEKIELDAKKKEYVAKKDKAKLSILKSMGVRTL
                     GYPCSKFDDDYSRLDLSFRLAEEESGIADEVEKQLLTIPGVSCGDARQVVSCAFPFDS
                     EKTEIHPRIEWFVAEDLRLIKAINGQIKSGKLGEIAHLKFGCTETFLNSYLAMQVGRG
                     "
     gene            complement(3723..>4030)
                     /locus_tag="PKH10_08095"
     CDS             complement(3723..>4030)
                     /locus_tag="PKH10_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28124.1"
                     /translation="KDGSYTASYSIGLVQLATTGKCGYHSEAGLPVAQDGESFSFSIL
                     NDPTCELIFRDLVITPNEECSQIVVDSTIEGCVSCTEGCGCGGGSETCTQSFTLNRE"
BASE COUNT          947 a         1138 c         1000 g          945 t
ORIGIN      
        1 cgacgacaaa ctctacctgc cggtctacaa catgcacctc ctctacaagt accggttcga
       61 ggagggctat ttcccgcgct gttcgagcct ccgttcctcg gtctgggaga cgaccaagga
      121 gaaactgtcg cgcgagatcg aacaggtggc cgagaagata ctgcaactct acgccgaacg
      181 ggcggtcgaa caggggcggc cgctcccgac ctcgggcgag atggtcgaac ggttcgccgc
      241 cgagttcccc taccgcgaaa cgatggatca atcccgcagc atctccgaac tggaacagga
      301 tctctcctcg ggccgcatca tggaccgtct gttgtgcggc gatgtcggct tcggcaagac
      361 cgaggtggcg atgcgcggat gcatggtgac ggcggcggcg ggtcgacagg cggcggtgct
      421 cgtccccacg accgtactcg gcttccagca ctggcagacc ttcaccgagc ggttccgcaa
      481 tttcccggtc cgcgtcgaac tcctttcgcg tttccagtcg gccgccgaac agaaaaagat
      541 actcgtcggg ctgaaggagg gctcggtcga tgtggtcatc ggcacccacc gcctcctttc
      601 caaggatgtc gagttccgcg atctcggctt cctcgtggtc gacgaggagc accgcttcgg
      661 cgtcgggcag aaggagcgca tcaaggagat gaaaaagggg gtggcgacgc tctcgatgac
      721 cgcaacgccg ataccgcgca ccctgcagtt gtcgctcctc ggcgtccgtg acatatcgtt
      781 catccgcacc ccgccgcgcg aacggaagcc ggtgaggacc catgtggtcg agtacagcga
      841 ggagatcgtc cgcgaggcga tactgcgcga gatggagcgc gacggacaga tatatttcct
      901 ccacaaccgc atagaatcgc tctcgacgat gaagcgcctg ctcgaaaaac tggtgcccga
      961 cctcaagata gcggtcgccc acggccgcat ggaggaggac cagttggaac aggtgatgct
     1021 cgacttcacc gcgcgccgct tcaatctcct gctcgccacc tcgctcatcg aatcgggcat
     1081 cgacataccg ctcgtcaaca ccatcgtcat caaccgcgcc gacaccttcg ggctcgccca
     1141 actctaccag ttgcgcggcc gcgtcggcag gtggaaccgc gaggccttcg cctatctcat
     1201 ggtcccctcc ttcaagacgc tcaccaagga cgcctatgac cggctgtcgg tcatcaaacg
     1261 gttcgaccgg ctcgggagcg gctacgatgt ggcgatggag gacctgaaca tacgcggcgc
     1321 gggcaatatc ctcggcctgt cgcagaccgg caaactcaag ggggtcggct atgacctgta
     1381 cctcgagatg ctccgccgcc gcatcgagtt cctccgcacc ggtcagaccg gcgagacgcg
     1441 cgacatcgaa gtgaagaccg atctccccgc cttcatcccg gagacctaca tcgaggacgc
     1501 cgagacgcgg gtcagtttct accgcaaact cgccgacacc gaacggcccg aggaactcgg
     1561 ctgggtgcgc catctgctgg cggagatgtt cggcccggtg ccgcccgagg tggagaatct
     1621 cttcacgctg aatgagatgc ggacgctcgc gcggaaggcg cgggtggtga agatggacct
     1681 gtcggaaggc cggatgacgc tcaccttctc ggcgaccgcc acacccaaga gcatggacga
     1741 cctgttcgag gcggtcgata tcctgcgcgg gaagttcgtg ccgccgagcg gcgtgcaggt
     1801 accggcgtca acgccggatg agtggagaaa ggcgctggag cggttcggca aggtgttcgg
     1861 gggaacgcct acttatcggt gaagaaaaat ttatcgaggt ggcaacacct tacgattctt
     1921 gatgtgccct attattgact tgacaagttc agcttgatca tcatttgcgt ttactacaaa
     1981 catcttcacg ccatgccttt ttgccaaatt tgccttccgc tcatctcgtt ctctgttttt
     2041 attaaaggca tccactccac caaagaactc aacaggttca taatgttgtt gcccgtgata
     2101 ctcaaccgca attttccaat tagggaacca aatatcaaaa tgctgcttgc cgagccatgg
     2161 aggttgtccg tgctggagaa cctgagtttg tgtaaatgac gattccaact tgcgaaatag
     2221 ctctgtttca gaaacccagc cctgtcctac ctggggaatt cccaattctt ctctcactcg
     2281 attctctgcc tctttagaca gcttcttgat ttcctccaga ttgccattca tcgaataaaa
     2341 agccgctata ccaaaagtca acttgggcga ttccattatt ggcacgccat taaaaaggga
     2401 atgtgaataa agcttggatg tgtcactcca tttatcgaaa atagagaacc accccccatg
     2461 cggatttgca aactgatcga aaatctcgat tatcttctct ttgtagagtg cttgattatt
     2521 cgaaagtatt tttgattttc tcccccccac cataagcaag atatcgatag gatttgcttc
     2581 aagatttaat ttcttttgta tgttcagacg aagattagat tgatgcgttg aagttccgaa
     2641 tagttctttg ggttctgttt tttccaagaa ttcttcgtat tgttgttgcc ccaacaaaca
     2701 atcatgagcc caaaacagac agtagttgga gaatttttgc tcgtttttat ataactcaga
     2761 caaatagatc agaaactcat gaagactttc aaaccctgca ttcctccatt tggcgagcaa
     2821 tgtatacaca tagacaaaca gatatgaaat gttaccatca acatcttgaa actcgcctcg
     2881 attcagtttt gtttcaagat ccttgtaaaa aatgcgttgt tgtgcattca tcgactcaat
     2941 ggacggatag tattcgctaa atttcggtat ttccaccata ttatcctctt ccaacttgca
     3001 tggcgagata tgaatttaga aaggtttctg tgcaaccgaa cttcaaatgg gcaatttctc
     3061 ccaacttgcc agatttaatt tggccattaa tagccttaat caatctaagg tcctccgcca
     3121 caaaccactc tattcttgga tgaatttccg ttttctcgct atcaaaagga aatgcacagg
     3181 aaacgacttg tcttgcatcg ccgcaagata cgccgggaat agtcaaaagt tgcttctcca
     3241 cttcatctgc aatgcccgat tcctcctccg ccaacctgaa agacagatca agtcgagaat
     3301 agtcatcatc aaatttacta caggggtacc ccaaagttcg cacacccatg ctttttagaa
     3361 tagatagctt cgccttgtcc tttttggcaa catattcttt tttcttggca tcaagttcta
     3421 tcttctcggt accgagcaac atttcaacag gcgttatttc agatatccag acatcgatac
     3481 ctctcttttc agccagatat cgcaaggatt gcagtgctga agaatggggg ccgttgtaac
     3541 tcccttcttg gattcggaaa agatggtcaa taacagaata atctaaaaaa attgaaggct
     3601 tttttgaaca agaaattgtt tctgccagac cgctcaccta ctaccttctc agtgaatgac
     3661 ccctgaagat acccccgccg ggaatgtgaa gcaagaaatt cttcagcccc gcccgaccac
     3721 cttcactccc ggttcagagt gaaactctgg gtgcaggttt ccgagccacc gccgcagccg
     3781 catccctccg tgcaggacac gcacccctcg atggtgctgt ccacgacaat ttgcgagcac
     3841 tcctcattcg gcgtgatgac gagatccctg aagatcaact cgcaggtggg atcgttgagg
     3901 atagagaagg agaaagactc cccatcctga gcgaccggca ggcccgcctc gctgtgatag
     3961 ccgcattttc ccgtagtggc gagttgcacc agcccgatgc tgtaacttgc cgtgtacgat
     4021 ccgtctttcc
//
LOCUS       DAOQUE010000245         4006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_25311_length_4006_cov_4.500633, whole genome shotgun sequence.
ACCESSION   DAOQUE010000245 DAOQUE010000000
VERSION     DAOQUE010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25311_length_4006_cov_4.500633"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PKH10_08100"
     CDS             complement(<1..528)
                     /locus_tag="PKH10_08100"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNT28125.1"
                     /translation="MKGILIGIFAITAVLFAVSCGSDGPETTDKDMSVSDTTQPDIDY
                     FGQGTSPEMVDIPAGDFQMGCNEAVDYQCYENESPYHAITLGAYKIGKYEVTVGEYQQ
                     CVTNGACNNSGDHLHYYTNTDSGYCNLGAEGKGNHPMNCVTWYGAKAYCEWIGGRLPT
                     EAEWELAARGTDERLY"
     gene            complement(663..1697)
                     /locus_tag="PKH10_08105"
     CDS             complement(663..1697)
                     /locus_tag="PKH10_08105"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT28126.1"
                     /translation="MLKGTNSLLSVAVMFALLALSCDNESTRERPRGTEGGPCYDDDT
                     CDGNLVCFEGMCFAPGAVSDKDALADDAAIPDIDHGTGTPGEMVDVPAGEFWMGCNEE
                     IEGPCQVSEYPYHAVTLSAYRIGRYEVTVGEYQKCIVSGACTNAGKDDHYYTFTESSF
                     CNIGTTGKDDHPANCVTWNGANAYCAWVGGRLPTEAEWEKAARGTDGWRCPWGNAGVS
                     CYYAVMDDENSGSEGCGTGGTLPVGSRPEGVSPYGAYDMIGNVTEWVADWFGEDYYAS
                     SPATDPPGPETGEWRVVRGGAWLDVDGKLVGYDEPLRASDRGAAYPGNFSLDIFPDPL
                     RDAVGFRCAE"
     gene            1839..3017
                     /locus_tag="PKH10_08110"
     CDS             1839..3017
                     /locus_tag="PKH10_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNT28127.1"
                     /translation="MTNSFRDLWPLDKERIHLNHGSFGACPTAILEHQRRLRDEMEAS
                     PTDFFCRRSPDLLTAVRIELSRFINADAEGMTFVPNATAGVNAVIRSHHWKKGDEVLT
                     TKLLYPACRNTLAFIAEEFGVKVNMVDIPYPPRSADDIVARIVAAATPNTRLALIDHV
                     TSSTALVLPIGRIVAELKKKGIPTLVDGAHGPGMTALDLTTIGAAWYTGNCHKWLCTP
                     IGSAFLWVALEEREQTYPLSISNYFYTPEGRAGFRAAFDWTGTADLTPFLCIGEGIRF
                     MGGLLPGGWDELRHRNNGLAREMAPIVATALKTTAPELGELNGSMITIPIPGLPYSFE
                     ANGLHPLMNRLYARHGIEAMITCIPDGRSALRISAQIYNGPADYERLAKALHNELFNG
                     "
     gene            3082..3696
                     /locus_tag="PKH10_08115"
     CDS             3082..3696
                     /locus_tag="PKH10_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28128.1"
                     /translation="MPLVLLFLIAIPVLTSAQDAAGGPWKEERSDENLVIHSRAVPGT
                     DIREVKATAIMTGTIKEAVDIIFDRKNHPGNMPYIKKSIVLSKDEKCDVSYNIVSPPV
                     ASNRDYIVRSCADVVSRYETKLWWETATHPDHPETDDNVRVKINKGYYIFRQVEPDKL
                     LVDYYIYTDPGGSLPIFVKNIANRTGVSDVLESLSKTILKRRKK"
     gene            3698..>4006
                     /locus_tag="PKH10_08120"
     CDS             3698..>4006
                     /locus_tag="PKH10_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28129.1"
                     /translation="MFLLDIFRPPLDDRKRTFFSHAAFMSFEEFVWGVFVLAEVVLRR
                     ELQASPFTITLFAILNPASSLFSVYFSQVLAAHPDRIYRHIRSVAVLTRLPLVLFFLW
                     H"
BASE COUNT          826 a         1339 c         1039 g          802 t
ORIGIN      
        1 ataaagacgt tcatcggttc ccctggcagc cagttcccat tcggcctcgg ttggcaatct
       61 gccgcctatc cattcgcagt aagccttcgc gccgtaccaa gtcacgcagt tcattgggtg
      121 atttcctttc ccttccgcgc cgaggttgca gtatccgctg tcggtgttgg tgtaatagtg
      181 gaggtggtcg ccgctgttgt tgcaagcacc gttggtgacg cactgctggt attcgccgac
      241 cgtcacttcg tacttgccga tcttgtaggc gccgagcgtt atcgcgtggt agggagattc
      301 attttcataa cattgatagt ccaccgcctc gttgcagccc atctggaaat ctccggcggg
      361 gatgtccacc atttctggag aagtaccttg cccaaagtaa tcgatgtcag gttgtgtcgt
      421 gtcgctcact gacatatctt tgtcggtagt ctcgggtcca tcgctgccgc aggaaacggc
      481 gaaaagaacc gccgtaatcg caaaaatgcc gatcaagatg cctttcatgc ttcccctcct
      541 ttcgtcgtgc cacaaaacag tgtcggttgc gaaaaaatca tcgtctcatt ggacgaatag
      601 tagacgaata ttagacgaat agcaagtaac cagttgcatt atctcgagaa taaccgctta
      661 tatcactccg cgcaccggaa cccaacggcg tcgcgcagag gatcgggaaa gatatccagc
      721 gagaagttcc cgggatatgc ggcccctcga tcagaagccc gcaaaggttc gtcatatcca
      781 acgagtttac cgtcaacatc aagccatgcc ccgccgcgca cgacacgcca ctcaccggtc
      841 tccggccccg gaggatcggt cgcgggcgat gaggcataat aatcttcgcc gaaccaatcg
      901 gccacccatt ccgtcacatt ccctatcatg tcgtaagcac catagggaga aacgccttcc
      961 ggcctgctcc ccaccggcaa ggtgcctcca gtaccgcatc cctcgctacc ggaattctca
     1021 tcatccatga cagcatagta gcaggaaaca ccggcgttac cccacggaca cctccagcca
     1081 tcggtcccgc gcgccgcctt ctcccattcg gcctcggtcg gcagacgccc gccgacccac
     1141 gcacagtaag cattcgcccc attccaggta acgcaattcg ccgggtgatc atctttcccc
     1201 gtcgtgccga tgttacagaa cgacgactct gtgaaggtat agtaatggtc gtccttcccc
     1261 gcgttggtac acgcgccgct gacaatgcac ttttgatact cgcccacggt cacctcgtat
     1321 ctaccgatcc tgtaggcaga gagtgtcacc gcatggtagg ggtactcgga gacctgacag
     1381 gggccctcga tctcctcatt acaccccatc cagaactctc cggcgggaac atcgaccatc
     1441 tcgcccggcg tgccagtacc gtggtcgatg tcgggaatgg ccgcatcatc ggcgagcgcg
     1501 tccttatcgg atactgctcc cggagcaaag cacatcccct cgaaacagac aaggtttcca
     1561 tcgcaggtgt cgtcatcgta gcagggaccg ccttccgtcc cccttggccg ctcccgcgtg
     1621 ctttcattgt cgcacgatag ggcgagaagc gcaaacatca cggcgaccga caagagcgag
     1681 ttcgttcctt tcagcacgga gtacctccca acagatcacg aacccatgtt agcacgctat
     1741 gagaataatt caagcggccc cgccggcatt ccctctcttc ttgcaaagcg acgccggttg
     1801 tggcagtatc gcccagaacg ggaagtacgg agtccgccat gaccaattcg ttcagagacc
     1861 tctggccgct cgacaaagag aggatacacc tcaaccacgg ctcgttcggc gcctgtccga
     1921 ccgcgatatt ggagcaccag cgccgcctgc gggacgagat ggaggcatcg cccaccgatt
     1981 tcttctgccg ccggtcgccc gacctgctca ccgccgtccg catcgagtta tcccgtttca
     2041 tcaacgcaga cgccgagggg atgaccttcg tccccaacgc caccgccgga gtgaacgcgg
     2101 tgatccgttc gcatcactgg aaaaaaggcg acgaggtgtt gacgacgaaa ctgctgtacc
     2161 ccgcctgccg caacactctc gccttcatcg ccgaagagtt cggcgtcaag gtgaacatgg
     2221 tcgacatccc ctacccgccc cggtcggccg acgacatcgt ggcgcgcatc gtcgcggcgg
     2281 cgaccccgaa tacccggctc gccctcatcg accatgtgac cagttccacg gcgctcgtgc
     2341 tccccatcgg ccgcatcgtc gccgaactga agaaaaaggg catcccgacg ctcgttgacg
     2401 gcgcccacgg ccccggcatg accgcgctcg acctcacgac gatcggcgcc gcgtggtaca
     2461 ccggcaactg ccacaagtgg ctctgcacgc cgataggttc cgcttttctc tgggtcgcct
     2521 tggaagagcg cgaacagacc tacccgctct cgatatcgaa ctatttctac actcccgaag
     2581 gacgcgccgg cttccgcgcc gcgttcgact ggaccggcac cgccgacctg acgccgttcc
     2641 tctgcatcgg cgagggaatc cgcttcatgg gcgggctcct gcccggcggc tgggatgaac
     2701 tacgccatcg caacaacgga ctggcgcggg agatggcgcc gatcgtcgcc acggcgctca
     2761 agacgacagc gcccgaactg ggcgaactca acggttcgat gatcaccatt ccgataccgg
     2821 ggctccccta ttcgttcgag gcgaacggac tccatccact catgaaccgg ctctatgccc
     2881 gtcacggcat cgaggcgatg atcacctgca tccccgacgg ccgctccgcc ctgcgcatat
     2941 cggcgcagat atacaacgga ccggccgact acgagcggct cgctaaggcg ctgcacaacg
     3001 aactttttaa cggttgacct tcttctttac tttctttttc ttttttgata cagtcgccca
     3061 aaactcacga gaggtgcgac gatgccgctt gtcctgcttt tcctcatcgc gatcccggtc
     3121 cttacctccg cccaagacgc cgccggcggc ccgtggaagg aggaacggag cgacgagaac
     3181 ctcgtcatcc acagtcgcgc cgtccccggg accgatatcc gcgaggtgaa ggcgaccgcc
     3241 atcatgaccg gcaccatcaa agaggcggtc gacatcatct tcgaccgcaa gaatcacccg
     3301 gggaacatgc cgtacatcaa aaagtccatc gttctgtcga aggacgagaa atgcgatgtg
     3361 tcctacaaca tcgtgtctcc cccggtcgcg agcaaccgcg actacatcgt taggtcctgc
     3421 gccgacgtgg tgagccgtta cgagaccaaa ttatggtggg aaacagcgac ccaccccgat
     3481 caccccgaga ccgacgacaa tgtccgcgtc aagataaaca aggggtacta catcttccgt
     3541 caggtggaac ccgacaaact gctggtggac tactatattt ataccgaccc gggcggttcg
     3601 ctccccatat tcgtcaagaa catcgccaac cggaccggcg tgtccgatgt gttagaaagc
     3661 ctctcaaaga ccattctgaa acggcggaag aaataaggtg ttcctcctcg acatcttccg
     3721 gccgccgctc gacgaccgca aacggacctt tttctcccac gccgccttca tgtcgttcga
     3781 ggagttcgtc tggggggtct tcgtgctggc cgaggtcgtc ctgcgacgcg agctgcaggc
     3841 aagccccttc acgataacgc tcttcgcgat actcaacccc gcctcgtcgc tcttttctgt
     3901 ctacttctca caggtgctcg cggcgcatcc cgaccgcatc taccgccaca tccgctcggt
     3961 cgcggtgctg acccgcctgc cgctcgtcct gttcttcctc tggcac
//
LOCUS       DAOQUE010000246         3998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_25384_length_3998_cov_4.151408, whole genome shotgun sequence.
ACCESSION   DAOQUE010000246 DAOQUE010000000
VERSION     DAOQUE010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25384_length_3998_cov_4.151408"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKH10_08125"
     CDS             <1..289
                     /locus_tag="PKH10_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28130.1"
                     /translation="DTPQPDDATEGDIPDETFPDDTMPDDAYPDDAFPDGPSDIDDQS
                     DLEPDVPSDFDFAGSDEIATDGTVTADEDTVVVPDSGKRAISTGCSCSLMF"
     gene            370..2802
                     /locus_tag="PKH10_08130"
     CDS             370..2802
                     /locus_tag="PKH10_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28131.1"
                     /translation="MRNMLLATAALAIILCTGASSAADGDERWVSMNPGLPGSSDGVS
                     AAVRYGDQIYIGGMFTFVGDTAAEKIARLDLSTGKWYPLAGGMSDGVDALAVGPDGTL
                     YAGGRFYQADGITVHHVARWDGTAWHALGNGLSDPVRALAVAPDGTLYAGGIFSATYD
                     GLKVNNIARWDGTAWHAMAGGTDNIVNELTVDADGTLYAGGLFGKAGGITARYVAKWQ
                     NGAWAPLGGGIDDGYVYGLAIDAQGALYVGGSFSKAAGVTVNHIAKWDGATWSPLGMG
                     TNAVIYSVKTATNGDLFVGGEFTEAGGVTVNRVARWNGTSWNALDTGMADEFIRDLVV
                     VPDGSVYAFGEFHFAGGVIAEHIAKWDGGVWSAIGSGINNWVHAIAVAPDGTVYAGGR
                     FSTTANSLANRIAQWDGTQWSPLGDGVDDTIFALAVDGAGTLYAGGDFITADGITVNH
                     IARWDGSSWSALDDGVNAPVLALAVDAGGDLYAGGSFMKAGDLTVGRVAKWDGVAWSA
                     LDTGTTSVVHALALHGVDLYAGGEFYHTLDEQGNDLFPADSIARWDGAAWNAVGDADT
                     FVDGAVYAVAVDGNGNLYIAGKVYPFNEWDGNIYVAKWNGAAWSLLGNGFVGDIPVFL
                     RALTVDGNGSLYAGGMFLRSNGVTVNHISRWDGAVWRPLGSGTNGLLYALAFDPKEDA
                     LYAGGLFKTAGGKTSPYVAKYLTGDVDIVVPDDDTAEADPVDDGTVIDNDTAVPDTDE
                     SDADTAQLDIDESDADIVQPDETTDETVDESADDEPTTDTDSPITTHDSLLTDTDPAK
                     PINEGCSCSLMF"
     gene            complement(2809..3618)
                     /locus_tag="PKH10_08135"
     CDS             complement(2809..3618)
                     /locus_tag="PKH10_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNT28132.1"
                     /translation="MEGSQHCEMNRLFVEQFADGELPFKESAEMSAHIASCDECRAHF
                     DATRRLRKILRATAAAGRLTAIERGGLEKLIADTAARHRTLRERIADLFASPYPLVAL
                     SASVALVAVIGVFLHQLLTVERSADLIVREILHIHEATLPDEFSTAGDIETVVKRGLD
                     LPAARVGRLVANNAVTKARFGTIGTRPMASLHVAGPAGNGTLLVARHNDALRKIFADS
                     DCLGADRCHARKRTMGDTDLLFWEEGEGDYLFVSKDRGMMNGMVQLVSADR"
     gene            complement(3596..>3998)
                     /locus_tag="PKH10_08140"
     CDS             complement(3596..>3998)
                     /locus_tag="PKH10_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNT28133.1"
                     /translation="FRVLKNNWLNRVSRDSARREIPDSDLRRTDEDEGFIPSAVGEEG
                     IAGDPILRDRVTAAFSQLPEEFREVCYLVDVEGYAYDETARLLNIPIGTVMSRLHRSR
                     EALKAKLLREAGELRIVPFRKEEKDGRIAAL"
BASE COUNT          724 a         1195 c         1330 g          749 t
ORIGIN      
        1 cgacaccccc cagcccgacg acgcgaccga aggcgacatc cccgacgaga ccttccccga
       61 cgataccatg cccgacgacg cttaccccga cgatgccttc cccgacggtc cttcggatat
      121 cgacgatcag tccgacctcg aacccgatgt gccgagcgac ttcgacttcg ccgggagcga
      181 tgagatagcg accgacggta cggtcaccgc cgacgaggac accgttgtcg tccccgacag
      241 cggcaaacgg gccatttcga ccgggtgcag ttgttcgctt atgttctgaa gcgaaagatc
      301 cctttgacaa tcctcccgat ttctgattag atcccgcaga atatctttca ttgaaccggg
      361 ggcagggcca tgcggaacat gcttcttgcg acggctgcgt tggctattat tctctgtacc
      421 ggtgcttcgt cggcggccga tggcgatgag cggtgggtgt cgatgaaccc cggccttccg
      481 gggagcagtg acggggtgag cgccgcggtc cggtacggcg atcagatcta tatcggcggc
      541 atgttcactt ttgtcgggga tacggcggcg gagaagatcg cgcggctcga tctctcgacg
      601 ggaaaatggt atccgctcgc cggcggcatg agcgacggcg tcgacgcgct tgcggtcggt
      661 cccgacggca cgctgtacgc aggcggcagg ttctatcaag ccgacggcat cacggtgcat
      721 catgtcgcca gatgggacgg caccgcgtgg cacgcgctcg gcaacgggct atccgatccg
      781 gtccgtgcgc ttgcggtcgc tcccgacggt acgctctacg ccgggggcat cttttcggcg
      841 acctacgatg gcctgaaagt gaacaatatc gcccgatggg acggcacggc gtggcacgcg
      901 atggcggggg gcacggacaa tatcgtcaac gaactgacgg tcgacgcgga tggcacgctc
      961 tatgccggcg gattgttcgg caaggcgggc ggcatcacgg cgagatatgt cgcgaagtgg
     1021 cagaacggtg catgggcgcc gctcggcggc ggcattgacg acgggtatgt gtacgggctg
     1081 gcgatcgatg cgcagggcgc actgtatgtc ggagggagtt tttcgaaagc ggcgggagtg
     1141 accgtcaacc atatcgcgaa atgggatggc gcgacatgga gtccgcttgg tatggggacg
     1201 aacgccgtga tctattccgt aaagaccgca acgaacggcg atctctttgt cggcggcgaa
     1261 ttcacggagg cgggcggcgt cacggtcaac cgggtcgccc gctggaacgg tacatcgtgg
     1321 aacgctttgg atacggggat ggccgacgag ttcatccgcg atctggtggt cgttccggac
     1381 gggtcggtct atgccttcgg tgaatttcat ttcgcggggg gcgtcatcgc ggagcatatc
     1441 gcaaaatggg acggcggcgt atggagtgcg atcggttccg gcataaacaa ttgggttcat
     1501 gccattgccg tcgcgcccga cggtacggtc tatgccggag gacgattctc gacgacggcg
     1561 aattcgcttg cgaaccgtat cgcgcaatgg gacggtaccc agtggagccc gctcggtgac
     1621 ggggtggacg atactatctt cgcgcttgcg gtcgatgggg cgggcaccct gtatgcgggg
     1681 ggcgatttca tcacggccga cgggatcacg gtgaaccata tcgcccggtg ggacggcagt
     1741 tcgtggagcg ctttggacga tggcgtgaac gctccggtgt tggcgcttgc ggtggatgcc
     1801 ggcggggatc tttacgccgg aggcagtttt atgaaggcgg gagatctcac ggtcggccgt
     1861 gtcgccaagt gggatggtgt tgcgtggagt gcgcttgata ccggaacgac ctcggtcgta
     1921 catgcgctgg ctttgcacgg cgtcgacttg tacgcaggcg gcgaattcta tcatacttta
     1981 gacgagcaag gtaacgacct tttcccggcc gatagcatcg cccggtggga tggcgccgca
     2041 tggaatgccg tcggcgacgc ggacaccttc gtggacggcg ctgtgtatgc cgtcgctgtc
     2101 gacgggaacg gcaacctcta catagcgggg aaggtctatc ccttcaatga gtgggacggg
     2161 aatatctatg tcgccaagtg gaacggcgcg gcgtggtctt tactggggaa cggttttgta
     2221 ggcgatatcc ccgtcttttt acgagccctt accgtcgacg ggaacggatc tctctatgcc
     2281 ggcggcatgt tcctgcggag taacggagtg accgtgaacc atatctcccg gtgggacggc
     2341 gcggtgtggc ggccgctggg aagcggcacg aacggactgc tctacgccct tgcgttcgac
     2401 ccgaaggagg atgcgctcta cgccggcggg ttgttcaaga cggcgggggg aaaaaccagc
     2461 ccctatgtgg cgaaatatct tacgggagat gtggatatcg tcgtacccga tgacgacacc
     2521 gccgaggccg atccggtgga cgatggcacg gtgatcgata acgacactgc cgttcccgac
     2581 accgacgaat cggacgccga tactgcgcaa ctcgacatcg acgaatcaga cgccgatatt
     2641 gtgcaacccg acgagacaac tgatgaaacg gtcgacgagt cggccgacga cgaaccaacc
     2701 accgacaccg actcacccat cacgactcac gactcacttc tcacggacac cgatcccgcc
     2761 aagccgatca acgaaggctg cagttgttcg cttatgttct gatggtggtc agcggtccgc
     2821 gctgacgagt tggaccatgc cgttcatcat gccacggtcc ttggaaacga acaggtaatc
     2881 gccttccccc tcttcccaga acagcaggtc ggtgtcgccc atcgtccgct tgcgggcatg
     2941 acagcggtcg gcgccgaggc agtcggaatc ggcgaatatc ttgcgcaggg cgtcgttgtg
     3001 ccgcgcgacg agcagggtgc cgttgccggc cggaccggcc acatggagcg acgccatggg
     3061 acgggtcccg atggtgccga accgcgcctt ggtcaccgcg ttgttcgcga caagacggcc
     3121 gacccgcgcc gcgggcagat caaggccgcg tttcaccacc gtttcgatat cgcccgccgt
     3181 ggagaactcg tcgggcagcg tggcctcgtg gatatgcagt atctcgcgca cgataaggtc
     3241 ggcggagcgt tcgacggtca gcaactggtg caggaacacc ccgatgaccg ccacgagcgc
     3301 taccgacgcc gacagggcga ccagcgggta cggcgaagcg aacaggtcgg ctatccgctc
     3361 gcggagcgtc cggtggcggg cggcggtgtc ggcgatgagt ttttcgagac cgccgcgctc
     3421 tatcgcggtc agccgcccgg cggcggccgt cgcccgcagg atcttcctga gccgtcgcgt
     3481 cgcatcgaaa tgggcgcggc actcgtcgca cgaggcgata tgggccgaca tctcggcgct
     3541 ttccttgaaa ggcagttcgc cgtcggcgaa ttgctcgaca aagagccggt tcatctcaca
     3601 gtgctgcgat ccttccatcc ttctcctcct tccggaacgg cacgatgcgc aattcgcccg
     3661 cctcgcgcag gagtttcgcc ttgagcgcct cccggctgcg gtgcaggcgc gacatgaccg
     3721 tcccgatggg tatgttcagc agtcgggccg tttcgtcgta cgcgtacccc tcgacatcga
     3781 ccagatagca gacctcccgg aactcttcgg gcaactgcga aaaggcggcg gtgacccggt
     3841 cgcgcaggat cggatcgccc gctatcccct cctcgccgac cgccgacggg atgaatcctt
     3901 cgtcctcgtc ggtgcgccgc aggtcgctgt cgggtatctc gcgccgcgct gagtcccgcg
     3961 acacccggtt gagccagttg ttcttgagga cgcggaac
//
LOCUS       DAOQUE010000247         3985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_25499_length_3985_cov_4.172265, whole genome shotgun sequence.
ACCESSION   DAOQUE010000247 DAOQUE010000000
VERSION     DAOQUE010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25499_length_3985_cov_4.172265"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PKH10_08145"
     CDS             complement(<1..615)
                     /locus_tag="PKH10_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28134.1"
                     /translation="MRPFHDDPLFTVRDERGLASFLGVPDISTLASSPVDLICEAFSR
                     LPYENLTKVIKRSDVIAPSSAQRLPDEVIADHLRYGTGGTCFSLTAALIAVLSRFGVA
                     AWPVLADRRYGTDTHCAVLLTLNGAPHLVDPGYLIHRPVALPDDMARRYENRFNTVEL
                     APAGPQRVDLFTLSGADRRYRLTYKTEAVDGTAFAAAWQRSFAWE"
     gene            complement(612..1253)
                     /gene="pth"
                     /locus_tag="PKH10_08150"
     CDS             complement(612..1253)
                     /gene="pth"
                     /locus_tag="PKH10_08150"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNT28135.1"
                     /translation="MPFLLVAGLGNPGFRYEKTRHNIGFRFVDAVQETAGFPPFSYEK
                     RFLAYMSRLDLEGKRILLLKPDTYMNLSGQAVRAVTDYFGISLDETVVVHDDLDIPFG
                     TVKMKTGGGGAGHRGIESLLQHLNGDAFARIRLGIGRPSEGLNGKDYVLLPFTAAEED
                     AVRRLWRPVWPDLFRALAAQGFAMAANRFANRSCLPPPPAPDPEQEPSAGEQG"
     gene            complement(1262..1981)
                     /locus_tag="PKH10_08155"
     CDS             complement(1262..1981)
                     /locus_tag="PKH10_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNT28136.1"
                     /translation="METVGMKVFPRTPGKKYVKGARREGLIPINVYGKLLKENIVGSV
                     ERGKIVKALRSPKGHNVLVSLETEDGKAVLAIPYRIDINPVKNIIDHIDFMAIKEDEP
                     ITVQVPLLKKGRSQGEIVGGRVLQVISEVVVRCRPADIPESLMVDVTPYDIGDRVMVN
                     DLPYPPGVTPMYRENVPAIVVNKGRGQMADEVAADGAAKPAEGAAAEAKPGEAAAAPA
                     ADKKGADDKKDAGKGDKGKKK"
     gene            complement(2005..2079)
                     /locus_tag="PKH10_08160"
     tRNA            complement(2005..2079)
                     /locus_tag="PKH10_08160"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2045..2047),aa:Gln,seq:ttg)
     gene            complement(2085..2948)
                     /gene="ispE"
                     /locus_tag="PKH10_08165"
     CDS             complement(2085..2948)
                     /gene="ispE"
                     /locus_tag="PKH10_08165"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNT28137.1"
                     /translation="MKDTFRSPAKINLRLDIVGRDETTGYHFLEMVLAPVSLSDEITV
                     EDADRTTIITEGCAERIPSEKNIVHRVIRAVEEAVGAPLPPLKVTIRKNIPTGAGMGG
                     GSGNGAAMLEYLDRRFTLHLGPERLMTIGARIGSDIPFFLARSPAVVTGFGEKVAPVR
                     LTAFPFPTLVVHPGFPVDTRQAYGLWDKKMLTKRGPVDTNIARVSVLRSLTEWVDFIR
                     NDLEAPVLEAYPALQVVRRRLTEEGAARVFMTGSGSAMVALFADDTACGAAERCLAGE
                     YPFVRKVKVLT"
     gene            complement(2959..3447)
                     /gene="coaD"
                     /locus_tag="PKH10_08170"
     CDS             complement(2959..3447)
                     /gene="coaD"
                     /locus_tag="PKH10_08170"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNT28138.1"
                     /translation="MERGKKRAIYPGSFDPITFGHIDIAQRSLKIFDQLIIAIGDNRN
                     KDPLFSKAQRIAQINEIFSDNDAVQVMAFDGLLVDLARQLEVFTIIRGLRAVSDFEFE
                     FQLSSFNRKMEPRVESFFMMTSEKYFYISSSMVRQVASLGGDISDFVPPNVIADLKNH
                     RR"
     gene            complement(3510..>3985)
                     /locus_tag="PKH10_08175"
     CDS             complement(3510..>3985)
                     /locus_tag="PKH10_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNT28139.1"
                     /translation="VQGRAGIHAFWSLDAAGSAEMLKLLPFCAVAVAASLFHRELDLM
                     SLGEETAAARGVDPARVSLLLFALCSFAVAGVVAVTGPIGFIGLMAPHIARLIVGPAH
                     RFLGPATLFTGAALLTLCDTAARTLIAPAEIPVGVLTALLGGPFFLWLLLRRTQH"
BASE COUNT          743 a         1243 c         1271 g          728 t
ORIGIN      
        1 ctcccacgcg aacgaccgtt gccacgccgc ggcgaacgcg gtgccgtcca ccgcctcggt
       61 cttgtaggtg agccggtagc gacggtcagc gccgctcagc gtgaacaggt cgacccgttg
      121 cggaccggcg ggcgcgagtt ccaccgtgtt gaaccggttc tcataacgcc gcgccatgtc
      181 atcaggcaag gcgaccggac ggtggataag atagccgggg tccaccaagt gcggcgcacc
      241 gttcagagta aggagcaccg cgcagtgggt atcggtgccg taccggcgat cggcgagtac
      301 cggccacgcc gcgacaccga atcgcgacag gaccgcgatg agcgccgcgg tcagcgaaaa
      361 gcaggtgccg ccggtaccgt accggagatg atcggcgatg acctcgtcgg gaagacgctg
      421 ggcgctggac ggcgctatga catcgctccg ctttatcact ttggtcagat tctcgtaggg
      481 caggcgggaa aaggcctcac agatcagatc gaccggagaa gaggcaaggg tcgaaatatc
      541 gggaacgccg aggaacgagg caagtccccg ctcgtcgcgc accgtgaaga gcgggtcgtc
      601 gtggaacggc ctcatccctg ttctccggcg gacggttctt gctccggatc cggcgcaggc
      661 ggcggaggca gacaggagcg gttggcgaac cggttggccg ccatggcgaa cccctgcgcg
      721 gcaagggcac ggaacaggtc gggccatacc gggcgccaga gccgcctcac cgcgtcctct
      781 tcggcggcgg tgaaggggag aagcacatag tccttgccgt tcagcccctc ggaggggcgg
      841 ccgatgccaa gccggatgcg ggcgaaggcg tcgccgttca ggtgctggag gagcgactct
      901 atcccgcggt gtcccgcgcc acccccgccg gtcttcatct tgaccgttcc gaacgggatg
      961 tcgagatcgt cgtggaccac caccgtttca tcgagcgata tgccgaaata gtcggtgacg
     1021 gcgcggaccg cctgtcccga aaggttcatg taggtgtccg gtttcaggag cagtatccgc
     1081 ttcccctcga gatcaagccg cgacatatac gcgaggaatc tcttctcgta ggaaaacggg
     1141 ggaaaaccgg cggtctcctg caccgcatcg acgaaacgga accctatgtt gtgtctggtc
     1201 ttttcgtaac ggaacccggg gttgccgagt cccgctacca gcaggaaggg catggagggc
     1261 gttatttctt tttcccttta tcgcctttgc cggcatcctt cttgtcatcg gcgcccttct
     1321 tgtcagcggc cggagcggcg gcggcttcac cgggcttcgc ctcggcggcg gcgccttcgg
     1381 ccggcttcgc ggcgccgtcg gcggccactt catcagccat ctgaccgcga cctttgttga
     1441 cgacgatggc cgggacattc tcgcgataca tcggcgtgac gccgggggga tacggcaggt
     1501 cgttgaccat cacccgatcg ccgatgtcat acggggtgac atcgaccatc aaactctcgg
     1561 ggatatcggc cggacggcac cggaccacca cttcgctgat gacctgcagc acgcgcccgc
     1621 cgacgatctc gccctgcgag cggccctttt tcaggagcgg cacctgcacc gttatcggtt
     1681 cgtcctcttt gatggccatg aagtcgatgt gatcgatgat gttcttgacg gggttgatgt
     1741 ctatgcggta cgggatggca agcaccgcct tgccgtcctc ggtctcaagc gagacgagga
     1801 cattgtggcc tttgggggaa cggagcgcct tgacgatctt gccgcgctcc accgagccga
     1861 cgatattctc cttcagcaac ttaccgtaca cattgatcgg gataagcccc tcgcggcggg
     1921 cccccttgac atatttcttc ccgggcgtcc tcgggaacac tttcatgccg acggtctcca
     1981 tggtttcctc cttactctca aaaatggctg gggcgggagg attcgaaccc ccgaatgccg
     2041 gaatcaaaat ccggagtctt tccgcttgac gacgccccaa tggtttatgt aagcaccttt
     2101 actttccgta cgaacgggta ctccccggcg agacagcgtt ccgcggcccc gcaggcggta
     2161 tcatccgcga acagagcgac catcgccgac ccgctcccgg tcatgaatac ccgggcggcg
     2221 ccctcttccg tgaggcgtcg ccgaacgacc tgcaacgccg ggtatgcttc gagtaccggc
     2281 gcctcgagat cgttccgtat gaaatcaacc cattctgtca aagaacggag aacggacacg
     2341 cgggcgatat ttgtatccac cggtcccctt tttgtcaaca tttttttatc ccacaggcca
     2401 tacgcctgcc gcgtatcgac cgggaagccg gggtgcacca cgagggtcgg gaagggaaaa
     2461 gcggtcagcc ggaccggcgc caccttctcg ccgaaaccgg tgaccaccgc cggggaacgg
     2521 gcaaggaaaa acgggatatc gctcccgatg cgggcgccga tggtcataag ccgctccgga
     2581 ccgagatgga gggtgaaccg gcgatcaaga tattcgagca tcgccgcgcc gttgcccgac
     2641 ccgccgccca tgccggcgcc ggtcgggata tttttccgga tggtgacctt gagcggcggc
     2701 agaggtgcac cgaccgcctc ctccaccgcc cggatgacgc gatggacgat gttcttttcc
     2761 gacgggatac gctccgcgca cccttcggtg atgatcgtgg tccgatcggc atcctcgacc
     2821 gttatctcgt cggaaagcga taccggcgcg agcaccatct cgaggaaatg gtacccggtc
     2881 gtctcgtcac gaccgacgat atcaagccgc agatttattt tggcggggga gcggaaggta
     2941 tctttcatgc gacggctgtc accgccggtg gttcttgagg tcggcgatca cattgggcgg
     3001 cacgaaatcg ctgatgtcgc cgccgagcga cgccacctgg cgcaccatgc tcgacgagat
     3061 gtaaaaatat ttctcgctcg tcatcatgaa gaacgactcg acgcgcggct ccatcttgcg
     3121 gttgaaactc gacagttgga actcgaactc gaaatcggac acggcacgca ggccgcggat
     3181 tatggtgaac acttcaagtt ggcgcgcaag atccacgagc aggccatcga acgccattac
     3241 ctgcaccgcg tcgttatcgc tgaatatctc gttgatctgg gcgatccgct gcgccttgga
     3301 gaagagcggg tccttgttgc ggttatcccc tatcgcgatg atgagttggt cgaatatctt
     3361 gaggctccgc tgggcgatgt cgatgtggcc gaaggttatc gggtcgaaac ttcccggata
     3421 gatggcccgc ttctttcccc gctccatcgt ctgcctcctt tcgctaccct cttcggtcgg
     3481 gtcattctat acggttaatg gggcgcgtgt caatgttgcg tccggcgcag gaggagccac
     3541 aggaagaagg gtccgccgag gagcgcggtg agcaccccga ccggtatctc ggcgggggcg
     3601 atgagggtgc gcgccgcggt gtcgcagagg gtgaggagcg ccgcgccggt gaagagcgtc
     3661 gccgggccga ggaaccggtg ggccggaccg acgatgagcc gcgcgatatg cggcgccatg
     3721 agcccgatga agccgatggg ccccgtcacc gcgaccaccc ccgcgacggc gaacgaacag
     3781 agggcgaaaa ggaggagcga cacccgcgcc ggatcgacgc cgcgcgcggc ggccgtctcc
     3841 tcgccgagac tcatgaggtc gagttcccgg tggaagaggg atgccgcgac ggcgacggcg
     3901 cagaacggca ggagtttcag catctcggcg cttcccgccg catcgaggct ccaaaaggca
     3961 tggatacccg ccctaccctg gacca
//
LOCUS       DAOQUE010000248         3978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_25546_length_3978_cov_4.915881, whole genome shotgun sequence.
ACCESSION   DAOQUE010000248 DAOQUE010000000
VERSION     DAOQUE010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25546_length_3978_cov_4.915881"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PKH10_08180"
     CDS             complement(<1..657)
                     /locus_tag="PKH10_08180"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNT28140.1"
                     /translation="MTDTTEQYKPLATSRDRRIAERWSLVLQAKEIPHTLANLPEGWT
                     LYIAEDDRERAERELSQYEQEERVRRAPLPSFPLYPAAVPGLVLVLCLLALFHSYTYA
                     PPGREFWLAAGRASAIRIGDGEWWRAFTALTLHSDLDHLGSNLVFGGVVLWALRRIIG
                     AGWAWLLVLAAGAIGNVFNAWFYGSAHNSIGASTAVFGAVGVLSAVQVLERFRLPRTR
                     I"
     gene            797..1441
                     /locus_tag="PKH10_08185"
     CDS             797..1441
                     /locus_tag="PKH10_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT28141.1"
                     /translation="MPYEQYARYAKWAALALFLLVAMGASSWGVGGAIAAFLALALFA
                     VLYAKDEIAVHPVTEDGPEKEVSRKKEKPAGKEAKDEKTVKPTARPEEPVRVSTVPGA
                     LPSAGNSIELKSMLFDRAGEEKKLLEAARSGDLALVTQLVEQGTALDAENPNGVTPLM
                     FAVRGGHAFVVAYLIEQGADVNKKTKSGVTPMKIAREAKAQEMMDLLAAGGALD"
     gene            1649..2506
                     /gene="hisG"
                     /locus_tag="PKH10_08190"
     CDS             1649..2506
                     /gene="hisG"
                     /locus_tag="PKH10_08190"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNT28142.1"
                     /translation="MATLRLVIPKGRIYEKVLKLMNDAGYRVSVQGRELKPVSGDPDI
                     ELKIMKPQNIPQLVALGSHDAGFAGLDWCVETGSAVETVLDLGFDPVRIVAAVPETVS
                     DADLRRKRIVVASEYERITRDWLDREKYDYHYLRVYGATEVFPPDDADMIVDNTSSGA
                     TLRENKLRIVGEVMRSSTRFIVNPAALTDPWKKNKIAEMKLLFASVLTAAKKVMLEMN
                     IPADKADAVIPKLPCMKAPTVAELAGGAGFAAKIVVNRDIVATLIPELIRLGATDILE
                     YEINKVIVN"
     gene            2518..3570
                     /gene="hisC"
                     /locus_tag="PKH10_08195"
     CDS             2518..3570
                     /gene="hisC"
                     /locus_tag="PKH10_08195"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNT28143.1"
                     /translation="MLPIRETIARMEPYQMEEDRTPFLRLDANENPYGPSPQVARAIA
                     AIPAADLARYPDRKPLRDAIAAREGLSVEQVELSNGSDDILRNLIDLTLRPGDAAVVT
                     APTFSMNILFLMVREARIVKVPFGPADPFPEEEVIAAVQKERANLLMLVNPGAPLGQP
                     IDPAAIERVVAANPDTLVVVDEAYGEFAGSTVAPLIRRYSNLVVTRTFSKAWGIAGMR
                     VGYCFADPAVIRPLRTALSPYPLSAAAVAAALAALGDEAWMRRCVKAICEERERMRAL
                     LAALGYTVFPSSANSLVITGGGLAATTAYLKERGILVRHFAKLPPLTGDALRISVGKA
                     EDTDRLIAALKERRSI"
     gene            3567..>3978
                     /locus_tag="PKH10_08200"
     CDS             3567..>3978
                     /locus_tag="PKH10_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HNT28144.1"
                     /translation="MKERKAQVARKTGETDITVKLNLDGTGAAQITVPVGMLGHMLES
                     LARHSGVDITLTAQGDTEVDQHHLVEDIGIVLGDAVRKALGDKKGINRAGFFVFPMDE
                     ALGVVALDIGGRAQLQYEAKFKRRFVGELDTDTVP"
BASE COUNT          769 a         1259 c         1317 g          633 t
ORIGIN      
        1 gatgcgggtg cgcggcaggc gaaaccgttc gagcacctgc accgccgaaa ggacgccgac
       61 cgccccgaac acggcggtcg aggcgccgat ggagttgtgc gccgagccgt aaaaccacgc
      121 attgaagaca ttgccgatcg cgccggcggc gagcacaagc agccacgccc agccggcccc
      181 gatgatgcgg cgaagcgccc acagcacgac accgccgaac acgaggttgg acccgagatg
      241 gtcaagatcg ctgtgaagcg tcaacgccgt gaaggcgcgc caccattcgc cgtcgccgat
      301 gcggatggcc gaggcgcgtc ccgccgcaag ccagaattcc cggccggggg gcgcataggt
      361 ataggaatgg aacagcgcga gcaggcagag caccagcacg agtcccggca ccgccgcggg
      421 ataaaggggg aacgacggca gcggcgcgcg gcggacccgc tcctcctgtt cgtactgtga
      481 cagttcccgt tccgcccgtt cccggtcgtc ttccgcgatg tagagcgtcc acccctcggg
      541 gaggttggcg agcgtgtgcg gaatctcttt ggcctgcagg accagactcc accgctcggc
      601 gatgcggcgg tcgcgcgagg tcgcgagcgg tttatattgt tcggtcgtgt cggtcatgcc
      661 gtaccctccg ggcccagtat accgatgcgc gcactaaatg ccagataagg agcgggcgat
      721 ggcgggcgac ttgctttttc cttccttttc ccgtacctta cgcgggtacc ggtccttcat
      781 gcaagggagg ttccccgtgc cgtacgaaca atacgctaga tacgccaaat gggccgcgct
      841 cgccctgttc ctgctcgtgg ccatgggcgc ctcctcgtgg ggcgtcggcg gcgcgatagc
      901 cgccttcctt gcgctcgccc tgttcgcggt gctctacgcg aaggacgaga tagcggtcca
      961 tccggtgacc gaggatgggc ccgaaaaaga ggtgtcgagg aaaaaggaaa aacctgcggg
     1021 aaaggaggcg aaggacgaaa agacggtgaa gccgaccgcg cgaccggaag agcccgtgcg
     1081 ggtgagcacc gtaccggggg ctcttccttc cgcagggaac tcgatagaac tcaagtcgat
     1141 gctgttcgac cgcgccggag aggagaagaa actgctcgag gcggcgcgaa gcggcgacct
     1201 cgcactcgtc acccaactcg ttgagcaggg gaccgctctg gacgccgaga accccaacgg
     1261 cgtcaccccg ctcatgttcg ccgttcgcgg cggtcatgcc ttcgtggtcg cctatctcat
     1321 cgagcagggg gctgatgtca acaagaagac caagagcggc gtcactccga tgaagattgc
     1381 ccgcgaggcg aaggcgcagg agatgatgga cctccttgcg gcaggcggcg cgcttgactg
     1441 aaaaatattt tttgacactc cgccttcttt cgcttatcat gctcgagtat tgaaacggag
     1501 tccccccatg aagaacatcg cgttcggcga caacagacat catcatgtgc acgatggcgg
     1561 accgttggcc tagctgaccg cgcgggtatg atatccacgg gccgtcggcg aaagccggcg
     1621 gctttttttt tgtccggagg aggagacgat ggcaacgttg agactggtga tcccgaaagg
     1681 gcggatctac gagaaggtct tgaaactgat gaacgacgcc ggctaccggg tgtcggtgca
     1741 ggggcgcgaa ctcaagccgg tgtcgggcga ccccgacatc gaactcaaga tcatgaagcc
     1801 gcagaacatc ccgcaactgg tcgcgctcgg ttcgcacgac gccgggttcg ccgggctcga
     1861 ctggtgcgtg gagaccggtt cggcggtcga gacggtgctc gatctcgggt tcgacccggt
     1921 gcgcatcgtc gcggcggtcc ccgaaacggt gagcgacgcc gacctgcgcc ggaagcggat
     1981 cgtcgttgca tccgagtacg aacggataac ccgcgattgg ctcgaccgcg agaagtatga
     2041 ttatcactat ctgcgggtct atggcgcgac cgaggtcttc ccgcccgacg acgccgacat
     2101 gatcgtcgac aacacctcgt cgggagcgac actgcgcgag aacaaactgc ggatcgtggg
     2161 cgaggtcatg cgctcctcga cgcggttcat cgtgaacccg gcggcgctca ccgacccgtg
     2221 gaaaaaaaat aagatagcgg agatgaaact gctgttcgca tcggtcctca ccgccgcgaa
     2281 gaaggtgatg ctcgagatga acatcccggc cgacaaggcc gacgccgtca tcccgaaact
     2341 gccctgcatg aaggcgccga cggtcgccga actcgcgggc ggcgccgggt tcgccgcgaa
     2401 gatcgtcgtg aaccgcgaca tcgtcgcgac gctgataccg gaactgatac ggctcggggc
     2461 gaccgacatc ctcgagtacg agataaacaa ggtcatcgtc aactgaagga ggcgctggtg
     2521 ctgccgatac gagagacgat agcgcggatg gagccctacc agatggagga ggatcgcacc
     2581 cccttcctgc ggctcgacgc gaacgagaac ccctacggcc cctcgccgca ggtggcgcgg
     2641 gcgatcgccg cgatcccggc ggccgacctc gcgcgctatc ccgaccgcaa gccgctgcgc
     2701 gacgcgatcg cggcccgcga agggctgtcg gtcgaacagg tcgaactgtc caacggctcc
     2761 gacgatatcc tgcgcaatct catcgacctc acgcttcgcc ccggcgacgc ggcggtcgtg
     2821 acggcgccga ccttctcgat gaacatcctt ttccttatgg tccgcgaggc gcgcatcgta
     2881 aaggtgccgt tcggtccggc cgacccattc cccgaagagg aggtgatcgc ggcggtccag
     2941 aaggaacggg cgaacctgct gatgctggtc aatcccggcg cgccgctcgg ccagcccatc
     3001 gaccctgcgg cgatcgaacg ggtcgttgcg gcgaaccccg atacgctggt ggtcgtcgac
     3061 gaggcctacg gcgaattcgc cggatcgacc gtggcgccgc tcatccgccg ctactccaac
     3121 cttgtggtga cgcggacctt ctcgaaggcg tgggggatcg ccgggatgcg ggtcggctac
     3181 tgttttgccg atcccgccgt catccgaccg ctccgcaccg cgctttcgcc ctatccgctt
     3241 tcggcggcgg ccgttgccgc ggcgctcgcg gcgctcggcg atgaggcgtg gatgcgccgg
     3301 tgcgtcaaag cgatatgcga agaacgggaa cggatgcggg cgctgctcgc ggcgctcggc
     3361 tacacggtat tcccctcgtc ggccaattcg ctcgtcatca ccggcggcgg acttgccgcg
     3421 acgacggcct atctcaagga acgcggcatc ctcgtgcgtc atttcgccaa actgccgccg
     3481 ctcacgggcg acgccctgcg gatatcggtc gggaaagcgg aggataccga ccggctcatc
     3541 gcggcactga aggaacggag gagcatatga aggaacggaa ggcacaggtc gcgcggaaga
     3601 ccggcgagac cgacatcacg gtgaaactga acctcgacgg cacgggcgcg gcgcagataa
     3661 cggtccccgt cgggatgctc ggccacatgc tcgaaagtct cgcgcggcac agcggcgtgg
     3721 acatcacgct cacggcgcaa ggcgacaccg aggtggacca gcatcacctc gtcgaggaca
     3781 tcggcatcgt gctcggagac gcggtaagaa aggcgctcgg cgataagaaa gggatcaacc
     3841 gcgccggttt cttcgtcttc ccgatggacg aggcgctcgg ggtggtggcg ctcgacatcg
     3901 gcggccgcgc tcaattgcag tacgaggcga agttcaaacg gcgcttcgtc ggcgaactcg
     3961 acaccgatac ggtgcccg
//
LOCUS       DAOQUE010000249         3978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_25552_length_3978_cov_3.309202, whole genome shotgun sequence.
ACCESSION   DAOQUE010000249 DAOQUE010000000
VERSION     DAOQUE010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25552_length_3978_cov_3.309202"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /gene="dnaG"
                     /locus_tag="PKH10_08205"
     CDS             complement(<1..1419)
                     /gene="dnaG"
                     /locus_tag="PKH10_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNT28145.1"
                     /translation="MNRIIEDRVSIVEVVKRYVKLQSSGRRLVGLCPFHKEKTPSFSV
                     NEEKQFYHCFGCGAHGNALTFVMNIEHLSFPEAVRKLGNEFGIRELLQERHDPSNDQA
                     EQDHRQIAAANRAAALFFHHKLNETPVALAYLQERGISLETIRRFGIGWIGNGDELIA
                     HLRTAAVSPKILEKSGLARLDGSGRLRSFFHDRIMIPVIDLRRQVIGFGGRIIGPGEP
                     KYLNTAETPVFLKRASLFGANLIRDGLREHRYIILTEGYFDVMALHAHGFTTAVAALG
                     TAIGAEHLKLLERFGVPVVMLLDGDAAGRKAMRRVLELELPEKLDLRAAFIESPDGKE
                     DPDSLVRGPNGPSRIEQLVSRAEGITQHYLSDLAEQALRDPNLPQRDLAKEEMARLVL
                     RYPAYRRGDHLNYIEELPRRIRTVSPDDAKKLRKETEAQMRELLKREKGRPGATRRSS
                     PEMSTDRSVARLKPISAHAVFQD"
     gene            1656..2117
                     /locus_tag="PKH10_08210"
     CDS             1656..2117
                     /locus_tag="PKH10_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNT28146.1"
                     /translation="MSTVAASLKKIEPDRSNLIKALQAVQEEEGYISNEAIAEVARHF
                     TVSAVEVEGVVSFYAQFKRVKPGKFKISVCDGTACHIKGASLVTGWISDELGIKGGET
                     DNEGLFSLEIVACLGCCSLAPVISINGKVYGNLDRKGLLKILKDHRKGGAA"
     gene            2114..3847
                     /locus_tag="PKH10_08215"
     CDS             2114..3847
                     /locus_tag="PKH10_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014012577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNT28147.1"
                     /translation="MKITRIKIGAGSCGIAAGATEVVDYFKKEVRDIPIVEVGCIGHC
                     YAEPLIEVETDKGSVFYEKVLPKKEHLDRILGLKDEGRLAIRSQRSEREKIKVLALAG
                     RIDMTSMDEYLANGGYEGLKKALAMKPADIVEEVKMSGLRGRGGGGFPTGMKWSFLAA
                     KEAKEKILICNADEGDPGAFMDRSLMESVPHQVLEGMLIGALATGATKLFIYCRAEYP
                     LAIRNLKIAIEQIYKHGLNKLNARTVEFRLKEGAGAFVCGEETAMIHSLEGKRGTPRF
                     RPPYPTDSGYHGHPTAINNVETFGNVPLILREGGKAFASVGTQGSKGTKLFALAGDVK
                     YPGLAEVPMGVTIGDIVYEVGGAEKGSVKAVQIGGPSGGCIPADKFDAVVDYDSLKSL
                     GAIMGSGGLIVIGNDRCMVETARYFLEFTARESCGKCTFCRIGTKRMLEKLEKITGGR
                     GTEEDVAFLADLGPKIIKGSLCGLGQTAPNPVISTLRYFRHEYDAHTKDHKCPALQCN
                     QLSDVKIHLDKCIKCGLCIKTCPVHAISKEFVVDNAICTRCNSCIEVCPKKTISRVEK
                     GTGYYGKSGGN"
     gene            3847..>3978
                     /locus_tag="PKH10_08220"
     CDS             3847..>3978
                     /locus_tag="PKH10_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNT28148.1"
                     /translation="MSITITIDKKPFAAEKGETILQVAERNGISIPTLCFNQKITKTT
                     "
BASE COUNT          838 a         1103 c         1278 g          759 t
ORIGIN      
        1 gtcctgaaag acggcgtgag cggatatcgg cttcagacgg gctaccgagc ggtccgttga
       61 catttcaggg gaggaccgtc gtgtagcgcc ggggcgtccc ttctcccgtt tcaggagttc
      121 gcgcatctgc gcctctgttt ccttgcgcag tttcttcgca tcgtcggggc tgacggtgcg
      181 gatgcgccga gggagttcct cgatatagtt caggtgatcg ccgcggcgat aggccgggta
      241 gcggagcacg agccgcgcca tctcctcttt cgcgaggtcc cgctggggga ggttcgggtc
      301 gcggagcgcc tgttcggcga ggtcgctgag gtagtgctgg gtgatgcctt cggcacggct
      361 gacgagttgt tcgatgcggg aagggccgtt cgggccgcgg acgagggaat cagggtcctc
      421 cttgccgtcc gggctttcaa tgaaggcggc gcgcaggtcg agtttctcgg gtagttccag
      481 ttcgagcacc cgccgcatag ccttgcgtcc tgcggcgtcg ccgtcgagta gcatgacgac
      541 cgggacaccg aaccgttcga gcagtttcag gtgctccgcg ccgatggcgg tgccgagcgc
      601 cgcgaccgcg gtggtgaagc cgtgggcatg gagcgccatg acatcgaagt atccttccgt
      661 gaggatgatg tagcggtgct cgcggagccc gtcgcggatg aggttcgcgc cgaaaagact
      721 ggcccgtttg aggaataccg gcgtttcggc ggtgttgaga tatttgggct cgcccggccc
      781 gatgatgcgg ccgccgaagc cgatgacctg ccgccgcagg tcgatcaccg gtatcatgat
      841 gcggtcgtgg aagaacgagc gaagtcgacc ggagccgtcg agccgggcta atccgctctt
      901 ttcaagtatc ttcggcgaaa cggcggcggt tcgcaggtga gcgatgagtt catcgccgtt
      961 gccgatccag ccgatgccga accgccggat ggtctccagc gagatgcctc gttcctgcag
     1021 ataggcgagg gcgacgggcg tctcgttcag tttgtggtgg aagaataatg cggcggcgcg
     1081 gttggccgcg gctatctggc ggtggtcctg ctccgcctga tcgttcgagg ggtcgtgccg
     1141 ttcctgcaac agttcgcgga tgccgaactc gttgccgagt ttacggaccg cttcggggaa
     1201 agaaaggtgc tcgatgttca tgacgaaggt gagggcattg ccatgggccc cgcagccgaa
     1261 acagtgatag aactgtttct cctcattgac gctgaacgag ggggtcttct ctttgtggaa
     1321 cgggcaaagc ccgacaaggc ggcgacccga cgactgtagc ttgacataac gtttgaccac
     1381 ctcaacgatg cttacacggt cctcaatgat gcgattcact tcaatgaacg acaagaggga
     1441 ccccgctggg caaaaaaatt cggaggcatt atattagggg aagagtaaaa gtcaaaaatt
     1501 gccaaaaaaa tagagtgggc tttttccggg gtggcggccc ctttagggtt ttgttagatt
     1561 ccttcgcttt gacatcgttg cgcttgactt tcgtttagaa ttgtctgaat gatgagcgaa
     1621 gtgtcagcgc aaaccctgac agcaaaggag gacctatgag taccgtagcg gcgtcgctca
     1681 agaagatcga accggatcga agcaacctga taaaggccct gcaggcggtc caggaagaag
     1741 aaggctacat ctccaacgag gcgatcgccg aggtcgcccg gcatttcacc gtctcggccg
     1801 tcgaggtcga gggtgtcgtc agtttctatg cccagttcaa acgggtgaaa cccggcaagt
     1861 tcaagatatc ggtctgcgac gggaccgcct gccacatcaa gggggcatcg ctcgtcaccg
     1921 gctggatatc ggacgagctc ggcatcaagg gcggcgaaac cgataacgag ggacttttct
     1981 cgctcgagat cgtcgcgtgc ctcgggtgct gcagcctcgc gccggtcatc agcatcaacg
     2041 gcaaggtgta tggcaacctt gaccggaagg ggctcctcaa gatactcaag gaccaccgta
     2101 agggaggcgc ggcatgaaga taactcgcat caagatcggc gccggttcct gcggcatcgc
     2161 ggcgggagcg accgaggtgg tggattattt caaaaaagag gtcagggata tccccatcgt
     2221 tgaggtggga tgcatcggtc attgctatgc cgagccgctc atcgaggtcg agaccgacaa
     2281 ggggtcggtg ttctatgaaa aggtcctgcc caagaaggag catctcgacc gcatcctcgg
     2341 actcaaggat gaggggcggc ttgcgatacg gtcgcagaga tcggagcgcg agaagataaa
     2401 agtgcttgcg ctcgccgggc gtatcgacat gacctccatg gacgagtacc tcgcgaatgg
     2461 cgggtatgag gggcttaaaa aagcgctcgc gatgaagccg gccgatatcg tcgaagaggt
     2521 gaagatgtcg ggtctgcgcg gtcgcggcgg tggcggtttt ccgaccggta tgaaatggtc
     2581 gttcctcgcc gccaaggagg cgaaggagaa gatactcatc tgtaacgccg acgagggcga
     2641 cccgggcgca ttcatggacc gttcgcttat ggagtcggtc ccgcatcagg tgctcgaagg
     2701 gatgctcata ggcgcgctcg ccaccggcgc gaccaaactc ttcatctatt gccgcgccga
     2761 atatccgctc gcgatacgca atctcaagat cgccatagag cagatatata agcacggact
     2821 caataaactt aacgcccgta cggtcgagtt caggctcaag gagggggccg gcgccttcgt
     2881 ctgtggcgag gagacggcga tgatccactc gctcgaaggc aaacgcggca cgccgcggtt
     2941 ccggccgccg tacccgaccg acagcgggta tcacggtcac ccgaccgcga taaacaatgt
     3001 cgagaccttc ggcaatgtgc cgctcatcct gcgcgagggt ggcaaggcct tcgcctcggt
     3061 cggcacgcaa ggttcgaagg gcaccaaact cttcgcgctc gccggcgatg tcaagtatcc
     3121 ggggctcgct gaggtcccga tgggcgtcac catcggcgac atcgtgtatg aggtcggcgg
     3181 cgccgagaag ggctcggtca aggcggtcca gatcggcggt ccgtcgggcg gctgcatccc
     3241 ggctgacaaa ttcgacgcgg tggttgacta cgattcgctt aaaagcctcg gcgccatcat
     3301 gggctccggc gggctcatcg tcatcgggaa cgaccgatgc atggtcgaaa cggcccgata
     3361 tttccttgag ttcacggcgc gcgagtcatg tggcaaatgc accttctgcc gcatcggcac
     3421 taaacggatg cttgaaaaac tcgagaagat cacgggcggc agggggaccg aagaggatgt
     3481 cgcgttcctc gccgacctcg gcccgaagat cattaaaggg tcgctgtgcg gacttggcca
     3541 gacggcgccc aacccggtca tctcgaccct acgctacttc cgtcacgaat acgatgcgca
     3601 caccaaggac cacaagtgtc cggcgctcca atgcaaccaa ttgtccgatg tcaagataca
     3661 tctcgacaaa tgcatcaagt gcgggctgtg catcaagacc tgtccggtcc atgcgatcag
     3721 caaggaattc gtcgtcgaca acgcgatatg cacgaggtgc aattcctgca tagaggtatg
     3781 tccgaaaaag accatcagcc gtgtcgagaa ggggaccggc tactacggca agagcggagg
     3841 taactaatga gcatcaccat cacgatagac aaaaagccgt tcgcggccga gaagggcgaa
     3901 acgatcctac aggtcgccga acgcaacggc atctcgatcc cgacgctctg cttcaaccag
     3961 aagataacca agaccacc
//
LOCUS       DAOQUE010000250         3975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_25575_length_3975_cov_6.230357, whole genome shotgun sequence.
ACCESSION   DAOQUE010000250 DAOQUE010000000
VERSION     DAOQUE010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25575_length_3975_cov_6.230357"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..466
                     /locus_tag="PKH10_08225"
     CDS             245..466
                     /locus_tag="PKH10_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28149.1"
                     /translation="MTMTNVTIGLDARTMLLLKQVKPKNQSKYIRDAIAEKAQKELAR
                     QIDEYCDHLQKNKEDLDGFNKLSEEGWPD"
     gene            426..788
                     /locus_tag="PKH10_08230"
     CDS             426..788
                     /locus_tag="PKH10_08230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNT28150.1"
                     /translation="MVSTNSPKKDGPIKRGAIVIVQFSPSVGSETKDRHHAVVVSIDR
                     LNERSPVITVVPLSTKVEKFFPVFEVLVEETGKPAKVDCTQVRTISKERIIGIKGQVS
                     QQSLSEIGAKIKLALGLD"
     gene            1406..1549
                     /locus_tag="PKH10_08235"
     CDS             1406..1549
                     /locus_tag="PKH10_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28151.1"
                     /translation="MKTGLYNFMGNSVEYYEGEDVGYDLDSASDVPVEALALMGEYVC
                     PLD"
     gene            1562..2026
                     /locus_tag="PKH10_08240"
     CDS             1562..2026
                     /locus_tag="PKH10_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein E"
                     /protein_id="HNT28152.1"
                     /translation="MFKELTALLKPGADLVITITSPAEGKVTLAILRKHFADSLPPLV
                     VTASAEELDARFAELIMTPLQTTAVALSNSEQYEKLAKKMEEEKRAKLAIPKKNAAAP
                     QKPASKSAPPERADTGENSPSDDAEEEEAGPADGSRTPPTEATDTDQLSLFK"
     gene            2094..2297
                     /locus_tag="PKH10_08245"
     CDS             2094..2297
                     /locus_tag="PKH10_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein C"
                     /protein_id="HNT28153.1"
                     /translation="MEINQLPRIFKHKDLKLPDPGAEMTPERVLSFYANTYSELTVAK
                     LEGPVIEEGKAVYTFVTTLGTKG"
     gene            2346..3509
                     /locus_tag="PKH10_08250"
     CDS             2346..3509
                     /locus_tag="PKH10_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28154.1"
                     /translation="MREGYYGTQTARPDGRRPEGRPRSVTRRGGRDTAASYRPALLSG
                     PLDLSPIPAIETALRFEFLPEHGLRPRRFLDELYRIVSRHPGYDAAREPDLSPATPWW
                     RVAAHLLVTFRRLTDPRFDLRIIHEEEKGVSLQLHYAIETRYDYYLADVSFLPHLRRR
                     HRPLHDLTVRFLATLLRRTAIGDWREYLEQGIYHDWLSETDDEERSDRDRRALLSYYG
                     KKEGLPSRYHDLLHPERIETPEELVRRLDRLRPGGYPAPLRSLMETMRDTLPLLDAAD
                     ISLAALAYPLTQEELDEQGSYPVQPDEYIALVWNDHRTLTVDDIQQNHHYHIESTGNE
                     YGLWPLGGVLICRPDEPYEIRKRDLDAFNGILESLCRFTEGGQEIKKLFGVTD"
     gene            3631..>3975
                     /locus_tag="PKH10_08255"
     CDS             3631..>3975
                     /locus_tag="PKH10_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28155.1"
                     /translation="MKLNDLLREQLAPSQALIIYRRAVTDRSVSLNNEDTYLELHPII
                     TAGDRYALGPGAPLTKKQVIALAALAVTDRSTLLGGFLPAGQLLYLDPAPALPRLLWW
                     RPPEKRELLFDER"
BASE COUNT          920 a         1265 c         1080 g          710 t
ORIGIN      
        1 cttcatgggt aattccgtgg agtattacga gggcgaagat gtcggctacg acctcgattc
       61 ggcctcggat gtccccgtcg aggcgctcgc cctgatgggc gagtatgtct gctcactgga
      121 ctaggaatgc caaaagccaa caatacatag gaaaaaatat tgtcaaaaac atacataaga
      181 aacagtattg acaataaccg caatatcggt atcctgtacc tgtaggcatt cgatggagga
      241 ttacatgacc atgaccaatg ttacgatcgg cttggatgcc cggacgatgc ttctgcttaa
      301 gcaggtcaag ccgaagaacc agagcaaata catccgcgat gccatcgcgg aaaaagcgca
      361 gaaggaactc gctcgtcaga tcgacgagta ctgcgatcac cttcagaaga acaaggagga
      421 tttggatggt ttcaacaaac tctccgaaga aggatggccc gattaaaaga ggcgccatcg
      481 tcatagtgca attctcccct tccgtcggtt cggaaacgaa agaccggcat cacgcggtcg
      541 ttgtgtcgat agaccgcctt aacgagcgtt cccccgtgat aacggtggtt ccgctgtcga
      601 ccaaagtgga gaaattcttt cccgtgttcg aggtactcgt cgaagagacc ggcaagccgg
      661 cgaaagtcga ttgcacacag gtacggacca tctcgaaaga acgcatcatc gggatcaaag
      721 gacaggtgtc ccaacagtca ctatcggaga tcggggcaaa aataaaactc gctctcggtc
      781 tcgattaaca cctttttttg aggcgcctca caagaggcgc ttccctatca accccaacat
      841 taacccgctt cgggatcgta aaaagaccga agagcaaccg cccactcctc cgtctgccat
      901 agcttttaaa caaaggcaga ccctgccaat gacacggctg gtgaatgggc ggcgcaagac
      961 gcggggaagc gagagtttcg gcgagcgcga tgctcttcgc cgaccccctt tcttctttgg
     1021 tccggcaaag aagaaagagg ggaaagaaga aaccggttaa tgcccgccgg gtcaaccgcc
     1081 ggcgttgccg tatcggtgca tcaagaggag gctgcgcaac tctcttcgca ttcgcttcgt
     1141 tcaaacaggt gctcgccttt gagtgagtac tgatggcctc ttgatgaccc tcggttgaag
     1201 gcgggcggat tgcgatataa caaccgattc ctgtgcgcgg ggcgcacggg agaaatacaa
     1261 taacaacacc ccgcttcggg atcgcaagga gaccaaggcg caacgacgag gcaacgcagg
     1321 tgtagtacag ctacatcgag gcagccgagg aggcgcaaca ccggtatacg aacgagaccg
     1381 aagcggatca ccaatggaga agacaatgaa aacaggactc tacaacttca tgggtaattc
     1441 cgtggagtat tacgagggcg aagatgtcgg ctacgacctc gattcggcct cggatgtccc
     1501 cgtcgaggcg ctcgccctga tgggcgagta cgtctgtccg ctggactagg gaggtgacgc
     1561 catgttcaaa gaactcaccg cgctcctcaa accgggcgcc gacctcgtca tcaccatcac
     1621 ctcccccgcc gaggggaagg tcactctcgc gatcttgcgg aagcacttcg ccgattccct
     1681 gccgccgctc gtggtcaccg cgtcggccga agaactcgac gcccgcttcg cggaactgat
     1741 catgaccccg ctccaaacga ccgccgtcgc gctctcgaac tccgaacagt acgagaaact
     1801 ggcgaagaag atggaagagg agaagcgtgc gaaactcgcc attcccaaga agaacgccgc
     1861 cgcgcctcag aaaccggcct ccaagtcggc tccgcccgaa cgggccgata ccggggagaa
     1921 ctccccctcg gacgatgccg aggaggagga ggccggaccc gcggacggct cccggacgcc
     1981 gccgacagag gcgaccgaca ccgatcaact gtcgttgttc aagtaaccgc gccaagcgct
     2041 cgcgcccgtc gttgggtgcg ggcgctccgc gcacaatcaa gaaggagaac cccatggaga
     2101 tcaatcaact gccccgtatc ttcaagcaca aagacctgaa actgcccgac cccggcgccg
     2161 agatgacccc cgagagggta ctctcattct acgcgaacac ttactccgaa ctgaccgtcg
     2221 ccaagctcga agggccggtc atcgaggagg gaaaagcggt ctataccttc gtcaccacgc
     2281 tcggtacgaa ggggtagccc catgaatatc cgtgaataca tcgatcaact gccggaggcc
     2341 atgacatgcg agaaggatac tacggtactc aaacggcacg ccctgacggg cgccgtcctg
     2401 aaggaagacc gcgatcggtc acacgaagag gcggccggga taccgccgct tcttatcgac
     2461 ccgctcttct gagcggaccg ctcgaccttt cgccgatacc ggccatcgaa acggcgctcc
     2521 gtttcgagtt cctccccgaa cacggactcc ggccgcgccg cttcctcgat gaactttacc
     2581 gcatcgtatc gcggcacccc ggctatgacg ccgcacggga acccgacctt tcccccgcca
     2641 ccccgtggtg gcgggtagcg gcccacctgc tcgtcacctt ccggcgcctg acggatcccc
     2701 ggttcgatct ccgtatcatc cacgaggaag agaagggagt atcgctacaa ctccattacg
     2761 cgatcgagac acgctacgac tattatctcg ccgatgtctc cttcctcccc catctccggc
     2821 ggcgccatcg tcccctgcac gacctgacgg tccggttcct cgccacgctc ctgcggcgta
     2881 ccgccatcgg cgactggcgt gaatacctcg agcaggggat ctaccacgac tggctctcgg
     2941 agaccgatga cgaagaacgg tccgaccggg accgtcgggc gctcctttcc tactacggga
     3001 aaaaggaggg cctaccgtcc cgttaccatg atctcctgca tcctgaacgg atcgagacgc
     3061 ccgaagaact ggttcgccgg ctcgaccgcc tgcggcccgg cgggtacccc gccccgctcc
     3121 ggtccctcat ggaaacgatg cgtgacacgc tcccgctcct tgatgccgcc gacatctcac
     3181 ttgccgcgct cgcctatccc ctcacgcagg aggaactcga tgaacagggc agttaccccg
     3241 ttcaacccga cgaatatatt gcgctggtct ggaacgacca ccgtaccctt accgttgacg
     3301 atatacagca aaaccaccat taccacattg aatcgaccgg gaacgagtac ggcctctggc
     3361 cgctcggcgg cgtccttatc tgccgccccg atgaacctta cgagatacgg aaacgcgacc
     3421 tagatgcttt caacgggatc cttgaaagcc tctgccgctt tacggaaggc ggccaagaga
     3481 tcaagaaact ttttggggtg accgactgaa aagagagcgg agtccgcttc gggggtggga
     3541 aggggaaggt aagaccccgc tccccctttc ctataatccc cgtttcccgt aagtcaaccg
     3601 aaaaccatta acagggaggg acccttcccc atgaaactta acgacctcct gcgcgagcaa
     3661 ctcgcgccgt ctcaggcgct cattatttac cgtcgcgccg taaccgatcg gtcggtatcg
     3721 ctcaacaatg aggataccta ccttgaactc catccgatca taacggcggg cgaccggtac
     3781 gccctcgggc cgggcgcacc gctcaccaag aagcaggtta tcgctctcgc ggcgctcgcc
     3841 gtcactgacc gctccacgct cctcggcggg ttcctgccgg cgggacaact cctctatctg
     3901 gatcccgccc ccgccctgcc gcggctcctc tggtggcgtc cccccgagaa gcgcgaactc
     3961 ctcttcgatg aacgg
//
LOCUS       DAOQUE010000251         3970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_25638_length_3970_cov_4.603831, whole genome shotgun sequence.
ACCESSION   DAOQUE010000251 DAOQUE010000000
VERSION     DAOQUE010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25638_length_3970_cov_4.603831"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..3908)
                     /locus_tag="PKH10_08260"
     CDS             complement(165..3908)
                     /locus_tag="PKH10_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNT28156.1"
                     /translation="MTVAADKPFKTAWNTPTLVINQSAVSYTRSPWGRPTATPLKDGA
                     ENIIYTIPADDYYELGPNDFYEATDRLPAGTFTLDYGTAVGAPVRLKAWDKNGTELQM
                     EHNAPNGDGSWPRMALQSYNAPDVSLTALDSSCRETTAVKIQNVNWIATMNKKVQSNL
                     FTNPHTFSTVGYLQNSLNQDAPDLQESHFVPQQATERRGSPTWKKITSMAPSGVSDHA
                     MAYDSVRGRIVLFGGEQEFISNETWEWDGTDWTQLNPVIKPSARYGHAMAYDSARGKV
                     VLFGGNTGSSTYYDDTWEWDGANWIQKNPAAKPSARDSHAMVYDSIRGKVVLFGGSTV
                     SGINDETWEWDGTDWIQINSATKPSERSSHAMVFDSVQGKTVLFGGYYGGYETWEWDG
                     TNWAQLNPTTKPSVYGHAMAYDSIRGRVVLFGRSSGGAETWEWDGTNWIQFSPANKPT
                     ARSYAAMAYDSIRGKVILLGGYDSENICSQGSSDYCNDTWEWDGANWTQLSASIVANP
                     SARSSHAMVYDSVRDKVILFGGNTGGDETWAWSGVNWILLDPVSKPSARYGHAMAYDS
                     ARGKLVLFGGRYYSDETWEWDGVNWTKMEPTTKPSGRDNHAMAYDSVRGKVVLFGGYT
                     ATTPWKNDETWEWDGVNWTQMDPVIKPSARYNHAMAYDSAREKTILFGGTTGQYDAYY
                     NDETWEWDGTNWVQLNPATRPSPRNSHAMAYDSVHDKMILFGGSIGSSSYSNETWEWD
                     GTNWGQLNPSTMPSPRDNHAMTYDGGRGKVVLYGGGNNETWEWNGFNWSKKEIDNQPP
                     KPSARSGHSMAYDGSRRKIVLFGGHDYSQLCSGGVSPYCNDTWEWDGLNWYPLYSMNA
                     PSFRYGQAMVYDSARRKIVLFGGEADHYGASNETWEWDGIQWTKRTGENCDDTNCYVC
                     TSTTCPSGRSRHVMAYDMFREKTMLFGGINLSDNDETWEWDGVSWTQRITTTQPSKRN
                     SHAIAYDPIQKIMVVFGGREELGSGGYSMSSDSSTWNGEVWDSNRGLPSPLYGHAMAY
                     DSQRNKVILFGGDTIEDLTSNETWQMFYGTKLGYVTINWVQLNPPISPSARIRHSMVY
                     HDARRTMLLFGGESGSYGNTNNEFWELNGGADGHPAQIMNVAWESSGVTDDSTVTYQS
                     LSVLFNAGGLGDITGTPTNGVDLLTWKHDRWVTVASNTADPDNLAPVTWETSDPVEIQ
                     TLLNSGLRKFNFAVVPTAPNGFLTDMGKIATDYAEVIVRYSIP"
BASE COUNT          868 a         1106 c          902 g         1094 t
ORIGIN      
        1 ggaccccatt atcaatatca tgccaatgcc catccacatc acggcattcg ttttttgcgg
       61 agtgagattc attccggttc cccctcgatc gatgttcacc tatgaaacat acaccccgct
      121 ctctctaaaa tcaaagttgt ggtgggggag ggggaccctc ctgcctacgg gatcgaatac
      181 cggacaatca cctcggcgta gtcggtggct atcttgccca tgtctgtcaa aaatccgttc
      241 ggtgctgtcg ggacgacggc gaagttgaat ttgcgcaggc cgctgttgag gagcgtctgt
      301 atctcgaccg ggtcgctcgt ttcccatgtc accggcgcga ggttgtcggg atcggcggta
      361 ttcgacgcca ccgtgaccca gcgatcgtgc ttccatgtca gcaggtcaac gccattggtc
      421 ggcgtaccgg taatgtcgcc aaggccaccg gcgttgaaaa gaacggagag cgactgatag
      481 gtgacggtcg agtcatcggt gacgccagaa ctctcccatg cgacattcat gatctgtgcg
      541 gggtgaccat cggctccgcc atttaattcc caaaattcat tgtttgtatt accgtacgaa
      601 cctgattcgc caccaaacaa caacattgtc cttctggcat catgataaac catagaatgt
      661 cttattcttg cgctaggcga aattggagga tttaattgca cccaatttat tgtcacataa
      721 ccgagttttg ttccataaaa catttgccat gtttcattgc ttgttaaatc ctcaatagta
      781 tcccctccaa aaagaataac tttatttcgt tgactatcgt aggccatcgc atggccgtac
      841 agcggcgatg gtaatcctcg attagaatcc cagacttcgc cattccatgt cgacgaatca
      901 ctagacatag aatatcctcc agaccccagc tcctctctcc ctccgaatac taccatgatt
      961 ttctgaatgg gatcataggc aatagcatga ctgtttcgtt ttgaaggttg cgttgtagtt
     1021 attctttggg tccagcttac cccatcccat tcccaagttt cgtcgttgtc tgaaaggttt
     1081 attccgccaa ataacattgt tttttctcta aacatgtcat atgccatcac atgtcgagac
     1141 cgccctgaag gacatgtcgt agatgtgcag acatagcagt tggtatcatc gcaattctct
     1201 ccggttcttt ttgtccactg aataccatcc cattcccatg tttcattgct tgcaccgtaa
     1261 tgatctgctt cgcctccaaa gagtacaatt ttgcgccggg cactatcgta taccatagcc
     1321 tgtccataac gaaatgaggg ggcattcata gaatacaatg ggtaccagtt taaaccatcc
     1381 cactcccatg tatcattaca gtaaggtgat actccaccag aacatagctg tgaataatca
     1441 tgtccaccga aaagaactat ttttctgcga ctgccatcat aggccatgga atgcccggac
     1501 cgcgcagatg gttttggcgg ctgattatct atttcttttt tgctccaatt aaacccattc
     1561 cactcccatg tttcattgtt acctccccca tataacacca ccttaccacg accaccgtcg
     1621 taggtcattg catgattatc acgaggtgat ggcatagtgg aagggttcaa ctgaccccaa
     1681 ttcgtcccat cccattccca tgtttcattg ctatatgaag aactacctat acttcccccg
     1741 aacaggatca tcttgtcatg cacactgtcg taggccattg cgtggctatt ccgaggcgac
     1801 ggacgggtgg ctggattcaa ctggacccaa ttggtaccgt cccattccca tgtttcgtcg
     1861 ttatagtagg cgtcatattg accagttgtt ccaccaaaaa ggattgtctt ctcgcgagca
     1921 ctatcataag ccatcgcgtg gttatagcga gcagatggct ttatgaccgg atccatttga
     1981 gtccagttga cgccgtccca ttcccaggtt tcgtcgtttt tccaaggtgt tgttgctgta
     2041 tatccgccga acaggaccac ctttccacga acgctgtcat acgccatagc gtgattatca
     2101 cgacctgatg gttttgttgt cggctccatt ttggtccagt taacaccgtc ccattcccat
     2161 gtttcatcac tgtagtagcg accaccgaac agaaccaatt ttccgcgggc gctgtcgtaa
     2221 gccatcgcgt gtccgtatcg cgccgatggt ttgctgactg ggtctaacag gatccagttc
     2281 actccactcc atgcccatgt ttcgtcacct cccgtatttc cgccaaacaa tatcactttg
     2341 tctcgaacgc tatcgtaaac catcgcatga ctacttcgcg ctgatggatt agccacaatt
     2401 gatgcactta attgagtcca gttggcccca tcccattccc atgtgtcatt gcaatagtcg
     2461 gaggatcctt gagagcaaat attttcgcta tcatatcctc ccaacaggat caccttgcca
     2521 cgaatactgt cgtaagccat cgcagcataa gatcgggctg ttggtttgtt ggctggagag
     2581 aattgtatcc agttggtgcc atcccattcc catgtctcag cgccacctga agatcgcccg
     2641 aacaaaacca cccttccacg aatactgtcg taggccatcg cgtggccgta taccgatggc
     2701 ttggttgtcg ggttcaactg ggcccaattg gtgccatccc attcccaagt ctcatagcct
     2761 ccataatatc ctccgaacag aaccgttttc ccttgaacgc tgtcaaagac catcgcgtgc
     2821 gaggatcgtt ccgatggctt agtggcggag tttatctgga tccagtcggt accgtcccat
     2881 tcccaggtct cgtcgttgat gcctgaaacg gtagatcctc cgaagagaac taccttgcca
     2941 cgaatactgt cgtaaaccat cgcgtgactg tctcgtgccg agggtttggc ggcgggattt
     3001 ttctggatcc agttggcacc atcccattcc catgtatcgt cgtagtatgt ggatgacccg
     3061 gtgtttccac cgaacagcac tacctttccg cgggcgctgt cgtaagccat cgcgtggccg
     3121 tatcgcgccg atggtttaat aactggattt agctgtgtcc aatctgtacc gtcccattcc
     3181 catgtttcat tactaataaa ttcttgctca ccgccaaaca acactattct gccccgaaca
     3241 ctatcgtaag ccatggcgtg atcagacacc cctgaaggag ccatacttgt tatttttttc
     3301 caagttggag accctctcct ttctgttgct tgttgaggta caaagtggct ttcctgtaaa
     3361 tctggcgcat cttgattcaa ggaattctgc aaatatccta ccgttgaaaa ggtatgcgga
     3421 ttcgtgaaaa ggttgctttg tacttttttg ttcatcgtcg cgatccagtt gacattctgg
     3481 atcttgaccg cggtcgtttc gcggcaggaa ctgtcaaggg ccgtgagcga cacatcgggc
     3541 gcattgtaac tctggagcgc catcctcggc cacgagccgt caccgttcgg cgcgttgtgc
     3601 tccatctgca gttctgtccc attcttatcc cacgctttga gccgtaccgg cgcgccgacc
     3661 gccgtcccgt agtcgagcgt gaaagtccct gccggaagcc ggtcggtcgc ctcataaaag
     3721 tcgttcggtc ccagttcgta gtagtcgtcc gccgggatgg tgtaaataat gttttctgca
     3781 ccatctttga gaggcgtcgc cgtcggccgt ccccacggcg agcgggtgta actgacggcg
     3841 ctctggttga tgaccagtgt cggggtgttc catgccgtct tgaagggctt gtcggcggcg
     3901 accgtcaccg tcgtctcgtt ccccgcttca tccttcagcg caagttcaag ggcgtattct
     3961 cctatcatcg
//
LOCUS       DAOQUE010000252         3970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_25641_length_3970_cov_3.841890, whole genome shotgun sequence.
ACCESSION   DAOQUE010000252 DAOQUE010000000
VERSION     DAOQUE010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25641_length_3970_cov_3.841890"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PKH10_08265"
     CDS             complement(<1..363)
                     /locus_tag="PKH10_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28157.1"
                     /translation="MKELMKAFCLTATILLLVCCGGDPTDPDTDGDGMFYPDIDTDAK
                     SDADKVSCNYSSTDNKIKPSATELEFTGYYKTFGGTRYVTVTNRCLSCTGSGLTIGSA
                     EIVQSAEAGFSMSPNPVAV"
     gene            complement(368..1651)
                     /locus_tag="PKH10_08270"
     CDS             complement(368..1651)
                     /locus_tag="PKH10_08270"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT28158.1"
                     /translation="MRNLTHILLAAVLAAVLACGACTPTESVYEAYGKKIGPNGGKHE
                     GIHGVTLIVPPKALDQEITFTIYALDTDPFTNLSATLTNIYALSPMAFKFDLPVTIII
                     PYTEDDIPDLLDEIDLKPYFAIDRKNFEEIPEGEYEQNLESNIFKLSTKFLGYFHIGF
                     PKELVKGQNNETTGISEMVLIPKGNFEYGAPSGTPTGAVEATEGVLTGEGFNTVEMSE
                     YYIDKYEVTNAQYLACQNADVCTEPYEINSSRYPNYYYDPAYRNFPVLWVSWNQANTF
                     CQWAGKQLPTEAQWEKAARGETFRKYPWGEEEPQDNAENAIANYSALFADVTDVTSFD
                     SAISPYGVYNMAGNAAEWTATWYDIDSYYKKSDMADPLKDPAGPEEGGTTQKKVIRGG
                     SWVSLTTEMTTYSRDKAFPHYRYYNLGFRCVKDGE"
     gene            complement(1668..2990)
                     /locus_tag="PKH10_08275"
     CDS             complement(1668..2990)
                     /locus_tag="PKH10_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28159.1"
                     /translation="MKKTLLILVLLAPLWSQAVIVEDGDGFKVYDEQRSEEITYYFID
                     EIVPAKDVGTDGWVPSDCGGLFGSPFNININLHYGAFSEMELTGKGVAQWPESVKLSY
                     EGTINGGHALMDYGIEMTALYKICPPFGDGTEQAISEYLNFDLRFFDEKGFTPFLLDG
                     NDENPVSMEDSIQQFHIWDGTLEDIIGVPISVPIIGNIASMIHLSFDLDGIMYIEMFG
                     SRIALDEKAQRSIFSDNQYLVIDPPLVGTTLEVSAHYESLVYYETTLILTVTLTLKIP
                     IFELEFPIPINIPLYKDKKDWIFNSEKISFEFPAIFIEERAHQFLDTVPGDEDHWEFT
                     VQNIGTRALEGRILSNDPNFLVLPEEFLLDPGQSAAVTVFFKPYDEGVKKGVIKFYSN
                     DPVEPFTTVALFGYSSESGTFYSKVKEGEEPRYEIRSSGGCTITLMDE"
     gene            complement(3004..>3970)
                     /locus_tag="PKH10_08280"
     CDS             complement(3004..>3970)
                     /locus_tag="PKH10_08280"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT28160.1"
                     /translation="LCSDLNGDLPGCMVLVAAGGKNFSMGSDGESGWFGSEAYEAEKP
                     RHRVTLQKNFYLDKYEVTVAQYKHFLNDPNNLDWRIENASRGIEKCFGDTDYLKIWDE
                     DEQPAYLDGKKDRFPVITICWYAANAYCKWAGRRLPTEDEWEFAARAVGDCVCSAGDQ
                     ACIDANAECNEFPWGNEDVFNTASTPYRANFRNSGDPYEPSKLEKEKIDPAILAMMYP
                     APNLTWVGYFNGTVYPNFVSKKGESPYGVHDMSGNAEEWVATRFYYYTDLAEGGVPTP
                     VGDQRVVRGGSWASTRHLIRTSYRRGVNPQNNSNMIGFRCAMDAN"
BASE COUNT          741 a         1140 c         1180 g          909 t
ORIGIN      
        1 gaccgccacc ggattgggcg acatcgagaa gcccgcctcc gcgctctgca cgatctcggc
       61 gctccctatc gtgagcccgc tcccggtgca ggagaggcac cggttggtga cggtcacata
      121 gcgggtgccg ccgaaggtct tgtaatagcc ggtgaactcg agttccgtcg cgctcggctt
      181 tatcttattg tcggtgcttg agtagttgca cgacaccttg tcggcgtcgc tcttcgcatc
      241 ggtgtcgatg tcgggataga acatgccgtc gccgtcggtg tcggggtcgg tcggatcacc
      301 gccgcaacag acaagcagaa ggatggtcgc cgtaagacag aaggctttca taagttcttt
      361 catggcgtca ctccccatcc ttgacacaac ggaagccgag gttgtagtag cggtagtgcg
      421 gaaaggcctt gtcccgcgaa taggtcgtca tctcggtcgt gagcgacacc caactgccgc
      481 cgcggatgac ctttttctgc gtcgtgccgc cttcctcggg acctgccggg tccttcagtg
      541 gatcggccat gtcgcttttc ttgtagtagg aatctatgtc gtaccaggtc gcggtccatt
      601 cggcggcgtt gccggccatg ttgtagacgc cataggggga aatggccgag tcgaacgagg
      661 tgacatcggt gacatcggcg aacagcgccg aatagttggc tatcgcattc tcggcgttgt
      721 cctgtggctc ctcctctccc cacgggtatt tccggaaggt ctcgccgcgc gccgcctttt
      781 cccactgggc ctcggtcggc agttgtttgc ccgcccactg acagaaggtg ttcgcctgat
      841 tccaactcac ccagagcacc ggaaaattac gataggcggg atcgtaatag tagttcggat
      901 acctcgatga gtttatctcg tagggctcgg tgcagacatc ggcattctga caggccaggt
      961 actgggcgtt ggtgacctcg tacttgtcga tgtagtactc ggacatctcg acggtgttga
     1021 acccctctcc cgtcagcacc ccttcggtcg cctcgaccgc gccggtcggc gtgccggacg
     1081 gggcgccgta ttcaaagttc cctttcggta tcaggaccat ctcggatatg cccgtcgtct
     1141 cgttgttctg tcccttcacg agttccttcg ggaacccgat atggaagtag ccgaggaact
     1201 tggtcgagag tttgaagatg ttgctttcga ggttctgctc gtattctccc tccggtatct
     1261 cctcgaagtt cttccggtcg atcgcgaaat agggcttgag gtctatctcg tcgagcaggt
     1321 cgggtatatc gtcctcggtg tacgggatga ttatcgtcac cggcaggtcg aacttaaagg
     1381 ccatcggcga gagtgcgtat atattggtga gcgttgcgga gaggttggtg aacggatcgg
     1441 tgtcgagcgc gtagatggtg aaggtgatct cctgatcgag cgccttgggg ggaacgatga
     1501 gcgtgacgcc gtggatcccc tcgtgcttgc cgccgttcgg accgatcttc ttgccatagg
     1561 cctcatagac cgattcggtg ggggtgcagg cgccgcatgc gagcacggca gccagcacgg
     1621 cagccagcag gatatgggtc aggttcctca tagcgtggta tcctccccta ctcgtccatc
     1681 agcgtaatcg tacaaccgcc ggaactccgt atctcgtaac ggggctcctc gccctccttg
     1741 accttgctgt agaaggtccc cgactcgctc gaatacccga acagggcgac cgtagtgaac
     1801 ggctccaccg ggtcgttcga atagaacttg atgacccctt tcttcacccc ttcgtcatac
     1861 ggtttgaaaa agacggtcac tgcggcgctc tgccccggat cgagcaggaa ctcctcgggg
     1921 agaacaagga agttcggatc gttcgaaagg atgcgtcctt cgagagcgcg ggtgccgatg
     1981 ttctgcacgg tgaattccca gtggtcctcg tcgcccggca cggtatcgag gaactggtgg
     2041 gcccgctcct cgatgaatat ggccgggaac tcgaaggata tcttctcgga attgaatatc
     2101 caatccttct tgtctttgta gagcgggatg tttatcggta tcgggaactc cagttcgaat
     2161 atgggtatct tgagcgtgag cgtgacggtg aggatgagcg tggtctcata atagacgagc
     2221 gactcgtagt gagccgagac ctctagtgtg gtgccgacga gcggcgggtc tatgacgaga
     2281 tactggttgt ctgagaatat cgaccgctga gccttctcgt cgagcgcgat gcgggaaccg
     2341 aacatctcga tatacatgat gccgtcaagg tcgaacgaaa ggtgtatcat ggaggcgatg
     2401 ttaccgatga tggggaccga gatgggaacc ccgatgatgt cctcgagcgt cccgtcccag
     2461 atgtggaact gctggatgct gtcctccatc gacaccgggt tctcatcatt cccgtcaagc
     2521 aggaacggcg tgaatccctt ttcgtcgaaa aaacgcagat cgaagttcag atactcactg
     2581 atggcctgtt cggtgccgtc accgaacgga ggacatatct tgtaaagagc cgtcatctcg
     2641 ataccgtagt ccatgagcgc atggccacca ttgatggtcc cctcgtagga gagtttcacc
     2701 gactcgggcc actgggcgac cccctttccc gtcagttcca tctcggaaaa ggcgccgtaa
     2761 tgaaggttga tgttgatgtt gaaggggcta ccgaagagcc cgccgcagtc gctgggcacc
     2821 caaccgtcgg taccgacatc ctttgccggc acgatctcat cgatgaagta ataggttatc
     2881 tcttcggacc gttgctcgtc atagaccttg aacccgtcgc catcctcgac gatgaccgcc
     2941 tgcgaccaga gcggcgccag aagcacgagg atcaacagtg tctttttcat ggccatacct
     3001 cgcttaattg gcgtccatcg cgcatcggaa gccgatcatg ttcgaattgt tctgcgggtt
     3061 gacgccgcgc cggtaactcg tccggatgag gtgccgggtg ctggcccaac tgccgccgcg
     3121 caccacccgc tggtcgccga ccggcgtcgg tacgccgccc tcggccagat cggtatagta
     3181 atagaagcgg gtggcgaccc attcctcggc gttccccgac atgtcgtgga cgccgtacgg
     3241 cgactcccct ttcttcgaca cgaaattcgg atagacggtg ccgttgaagt agccgaccca
     3301 ggtaaggttc ggcgcgggat acatcatggc gagtatcgcc gggtctatct tttccttttc
     3361 cagtttgctc ggttcgtacg gatcgccgct gttgcggaaa ttggctcgat acggggtcga
     3421 cgcggtgttg aacacatcct cgttgcccca cgggaactcg ttgcattcgg cattggcgtc
     3481 gatacaggcc tgatcgcccg ccgagcacac gcagtcgccg accgcacggg cggcgaattc
     3541 ccattcgtcc tcggtcggca ggcggcgtcc cgcccatttg caatacgcgt tggcggcgta
     3601 ccagcagatg gtgatcacgg ggaaccgatc ctttttgccg tccagatagg ccggctgctc
     3661 gtcctcgtcc catatcttca gatagtcagt gtcgccgaaa catttctcga tgccgcgcga
     3721 ggcgttctcg atgcgccaat cgagattgtt cgggtcattc aggaagtgtt tgtattgcgc
     3781 tacggtcacc tcgtatttgt ccagatagaa gttcttctgc agggtgacgc gatggcgcgg
     3841 cttctcggcc tcgtatgctt cggagccgaa ccagccgctc tcgccgtcgg atcccatcga
     3901 gaaattcttg ccgcctgccg cgacgagcac catgcatccc ggcagatcgc cgttcaagtc
     3961 ggaacagagc
//
LOCUS       DAOQUE010000253         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_25684_length_3966_cov_4.283815, whole genome shotgun sequence.
ACCESSION   DAOQUE010000253 DAOQUE010000000
VERSION     DAOQUE010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25684_length_3966_cov_4.283815"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKH10_08285"
     CDS             complement(<1..383)
                     /locus_tag="PKH10_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28161.1"
                     /translation="MRMHFYLVLSISLSIIFPLACATPEDDPYYCETTLDCADGWECV
                     DHSCIISVNTDDSDGGTNDKTLPEVDTPLPDADLPPVEDDAMPDDLTLDDTIGENEVD
                     EDIPPVDDVTDDTVTDDTVTDDTMT"
     gene            complement(547..2019)
                     /locus_tag="PKH10_08290"
     CDS             complement(547..2019)
                     /locus_tag="PKH10_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28162.1"
                     /translation="MKMPCERLALFLSGLFLFAFTACTPLEEEAYCETSYDCPDGWEC
                     GPEYVCVESGTDNSTGGNDNALPDTTGSDTAVDEDLPTDDDEAPVTDTEEDPLLDTEG
                     SIIDSDEPTTGDMEPSDTNDITPDSDNSVNECDQNNGGCAQNCADTVEGYTCSCDDGY
                     TLNADQHACDDIDECTVNTNPCDNNGDTPATCTNTPGSYTCTCTALRFEFTNGSCFDI
                     DECTAGTDNCAETGATCTNSVGLFSCACNDYYSGDGTACVFCNTDGQCGAVCVACEGT
                     TTHCKDNGDGTTQCVQCTDNAHCNTGAGESCNAQNICTACPLPLTLATWDSGDDGWTK
                     EGAWRREGSEMRWGSTTSYNSSYTHNLAYATDLSLAGCPSAVLTFQIQLKDDLWSSES
                     GTDKNQKIYVECSGNSGGSWVSLTPPTLPTAQTKSSGCTTYYCDGNSSIDRSFSKTGQ
                     TWTLPAECRTATARIRFRANGSTAWDLLNPGWIVDTVSLN"
     gene            complement(2089..>3966)
                     /locus_tag="PKH10_08295"
     CDS             complement(2089..>3966)
                     /locus_tag="PKH10_08295"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT28163.1"
                     /translation="DPIMAGDPIMAGDPIMSQVGHFSDFAVYVRNKNDIGLTCEEIYN
                     CAVPCGNDFECMDACKAEGSVNAQPLFQALVDCWMENCDAACGDGDPIACDSCGAENC
                     AAEIAACGVTLGADSDTAGRDDIATDDILPDADTVKNPDDLLGEWEFTTDNAHVSVMK
                     GETDDYMLDISFNSTEEFYYSHAGTFNGYTYTGDYTYWDGSTEKTAAMTVVFTLTAPN
                     TVNIEITAEAPMGNRTLTGGVREVYDEDTIQPDSDAIAPVCGNTVVETGESCDSTTAA
                     CADTLTGYTGNAYCCATTCTWNLDRCVLDTSCYGGSFFQYVSTFDLCVRVGTGVPPTE
                     QFSCYDTAGLISCPLAAETFAGQDAQYFGGKTKMLALYPESVYDQSTGLTWPSTFVSD
                     YSADGFCASPSGCTWTEAQAFCAGQNWEGASGWRLPTDREIDTILNFSISPMFDPDFF
                     FDIPANYGLWTAIEVPGMTGYAYQVYTHSGAFEPYDETSTAKVLCVNNTYYQPTMQAV
                     AYQRNNEPVWYDETTGLMWSPVLYTEVDWQNALWYCETAVYAGYNDWRLPTANELRTI
                     YDLSVGDFSGTEFPGNWQVEQWTSTTATYSPESAYVVNGMVGARYPAYKLNAYGVRCV
                     R"
BASE COUNT          756 a         1104 c         1239 g          867 t
ORIGIN      
        1 tcggtcatag tgtcgtcggt cacagtgtcg tcggtcacag tgtcgtcggt cacatcgtcg
       61 acgggaggga tatcctcgtc gacttcgttc tcgccgatcg tatcgtccag tgtcaggtcg
      121 tcgggcatgg cgtcgtcttc gaccggtgga agatcggcgt cgggaagcgg tgtgtcaact
      181 tcagggagcg ttttatcgtt cgtgccgcca tcggaatcgt ccgtgttgac cgaaatgatg
      241 caagaatgat cgacgcattc ccatccatcg gcacagtcga gcgtcgtctc gcagtagtag
      301 gggtcgtcct cgggggtggc gcaggcgagg ggaaaaatga tcgatagtga aatggaaagg
      361 acaaggtaaa aatgcatccg catgagccgc cctctttcag aaaaagaaaa ataccctagg
      421 attttaaagt atcgtgcggc aaaaagcaag cgttgtgctg gcgttttatt atggcagtga
      481 tccgggcgag taggtcgccg gatacacatc aggacaatat cgagacacga tccccgcccc
      541 ctttgatcaa ttcaacgaaa cagtatcgac gatccatccg gggttcagga ggtcccatgc
      601 ggtggatccg ttggcccgga aacggatacg ggccgtcgcg gtgcggcatt cggcgggtag
      661 tgtccatgtc tgacccgttt ttgaaaagga gcggtctata gaagagtttc cgtcgcagta
      721 ataggtggtg cagccgctgc tcttggtctg ggctgtcgga agggtcggcg gagtgagcga
      781 gacccaactg ccgccgctgt tcccggaaca ttcgacatag atcttctggt tcttgtcggt
      841 gcccgattcg ctcgaccaga ggtcgtcctt gagttggatc tggaaggtaa ggacggcgct
      901 cgggcatccg gcgagcgata gatcggtcgc ataggcgagg ttgtgggtgt aggaactgtt
      961 ataggaggtg gtcgatcccc accgcatctc gctcccttcc cgacgccaag ccccttcctt
     1021 ggtccaaccg tcatcgccgc tgtcccaagt tgcgagcgtc agcggcagcg ggcaggcggt
     1081 gcagatgttc tgggcattgc aagattcccc ggcgccggtg ttgcagtggg cgttatcggt
     1141 gcactggacg cactgggtcg tgccgtcccc gttgtccttg caatgcgttg tcgtgccttc
     1201 gcaggcaacg cagacggcgc cacactgacc gtcggtgttg caaaagacgc acgcggtacc
     1261 atcgccgctg tagtagtcat tgcaggcgca ggagaacaag ccgacgctgt tcgtgcaggt
     1321 cgcgccggtc tccgcgcagt tgtcggtacc ggcggtgcat tcgtcgatgt cgaagcagga
     1381 gccgttcgtg aattcgaacc ggagcgcagt gcaggtgcag gtgtaactgc ccggcgtatt
     1441 ggtgcaggtc gccggcgtgt cgccgttgtt gtcgcaggga ttggtgttca ccgtgcattc
     1501 gtcgatgtcg tcgcaggcat gctgatcggc gttcagcgta tagccgtcgt cgcaggaaca
     1561 ggtgtatccc tcgaccgtgt cggcgcagtt ctgcgcgcag cctccgttgt tctgatcgca
     1621 ctcgttgacg ctgttatcgc tatcgggcgt tatatcgttc gtatcagatg gttccatatc
     1681 gccggtcgtc ggttcatccg agtctatgat cgatccctcg gtgtcgagga gagggtcttc
     1741 ctccgtatcg gtcaccggag cctcgtcgtc atcggtaggc aggtcttcat ccacggcggt
     1801 gtctgatccg gtcgtgtcgg gaagtgcgtt gtcgttccct ccggtgctat tgtcggtgcc
     1861 ggactcgaca cacacatact cggggccgca ttcccacccg tcggggcaat catacgatgt
     1921 ttcgcagtag gcctcttcct cgagtggcgt acaggcggta aaagcaaaaa ggaaaagtcc
     1981 ggataaaaag agggcgaggc gttcacaggg catcttcatg ggtatactcc gctaaaagga
     2041 acgaaggata gtaacgcgaa aggcataaaa aacaatggct tttatctctt accgcacgca
     2101 acggacgccg tatgcgttca atttatatgc cggatagcgc gctcccacca tgccgttgac
     2161 cacataggcc gactcggggg aataggtggc ggtcgttgat gtccactgtt ccacctgcca
     2221 gttccccggg aattcggtcc cgctgaagtc gcccaccgac aggtcgtaga tggtccgcag
     2281 ttcgttcgcc gtcggtaggc gccagtcgtt ataccctgcg tacacggctg tttcgcagta
     2341 ccaaagggca ttctgccagt cgacctcggt gtagagtacc ggcgaccaca tcagtccggt
     2401 cgtctcatcg taccacaccg gttcgttgtt acgctgatag gcgaccgcct gcatcgtcgg
     2461 ctgataatag gtgttgttca cgcacagaac tttagcggtg ctcgtttcgt cgtaaggctc
     2521 gaaggcaccc gagtgggtgt agacctgata ggcgtagccg gtcatgccgg ggacctcgat
     2581 cgcggtccag agaccgtaat tggcggggat atcgaagaag aaatcgggat cgaacatagg
     2641 agaaatgctg aagttcagta tggtgtcgat ctcgcggtcg gtcggcaggc gccagccgct
     2701 tgccccctcc caattctgac cggcgcaaaa ggcctgtgcc tcggtccagg tgcagccgct
     2761 gggggaggca cagaaaccgt ccgcggagta atcgcttacg aaggtgctcg gccaggtgag
     2821 tccggtcgac tggtcgtaga ccgattcggg gtagagcgca agcatcttgg tcttcccgcc
     2881 gaaatactgc gcatcctgtc ccgcaaaggt ttcggccgcc agcgggcagg agatgagccc
     2941 cgcagtatcg taacaggaga attgttcggt cggcgggacg ccggtgccga ccctgacgca
     3001 caggtcgaag gtcgaaacat actggaagaa agaaccgccg tagcaggagg tgtcgagcac
     3061 acaacggtcg agattccacg tgcaggtggt agcacaacaa tacgcattgc cggtgtaccc
     3121 ggtaagagtg tcggcgcagg ccgctgtcgt gctgtcgcat gactcgcctg tttcgaccac
     3181 cgtgttaccg cagaccggcg cgatcgcgtc gctgtcgggc tggatcgtat cctcgtcgta
     3241 gacctcgcgc acaccgccgg tgagcgtccg gttccccatc ggtgcctcgg ccgttatctc
     3301 gatatttacg gtgttgggtg cggtcagggt gaagacgacc gtcatggccg ccgttttctc
     3361 ggtcgagccg tcccagtagg tatagtcgcc ggtataggtg tagccgttga aagtgccggc
     3421 gtgcgaatag tagaactcct cggtgctgtt gaaactgata tcgagcatat aatcgtcggt
     3481 ctcgcccttc atgaccgaca catgggcgtt atcggtcgtg aactcccatt cacccagcag
     3541 gtcgtcgggg ttcttgaccg tatcagcgtc gggcaggatg tcgtcggtcg cgatatcgtc
     3601 acgaccggcc gtgtcgctgt cggcgccgag cgtaacgccg caggcggcga tttccgcggc
     3661 gcagttctcg gcaccgcaac tatcgcacgc gatcgggtcg ccgtcgccgc aggcggcgtc
     3721 gcaattctcc atccagcaat cgaccagcgc ctggaagagc ggttgcgcgt tgacacttcc
     3781 ctctgccttg catgcgtcca tgcactcgaa gtcgttaccg cacggcaccg cgcaattgta
     3841 tatttcctca caggtcaggc cgatatcatt cttgttcctc acatagaccg cgaaatcgga
     3901 gaagtggccc acctgcgaca tgatgggatc gcccgccatg atggggtcgc ccgccatgat
     3961 ggggtc
//
LOCUS       DAOQUE010000254         3956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_25778_length_3956_cov_8.307613, whole genome shotgun sequence.
ACCESSION   DAOQUE010000254 DAOQUE010000000
VERSION     DAOQUE010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25778_length_3956_cov_8.307613"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2269)
                     /locus_tag="PKH10_08300"
     CDS             complement(<1..2269)
                     /locus_tag="PKH10_08300"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNT28164.1"
                     /translation="MKLIITEKPSMAKKYREALKGIPDLRVANSFGHIEALADINQYL
                     ADTLGENNTWRGSIPHLPFVPSKFVHVIREEEAFAEIAKHLVQAEEVVLACDPDREGE
                     LIHRNIMEIAADRGLLKARTLTRVWLHSETASEIRKAFEQRKPYTDYEGYYQAARTRE
                     AVDWTVGLQLTRLYSVKYGRPGHLISVGRVQSWLLAEIVKRWRENKEHKPQTFWTYSF
                     KTTDGVLFNLVDEEKKIRRFFAVDAEEQKKLVTIAGKPLAITAVEKKPFTEYAPALYD
                     LKQLQKDAANRYKFTPDDTLKIAQALYETHELISYPRTDCNVLSPEEAKELGKAVALV
                     ERFPDYRQLLATVRTMNPDLALAKKYIGELQGHYAIIPVLSYDRNGVPTLSEEERKVF
                     DLIVKRFLAALLPPAKGDTTEMLGKIEGFPFLARFKNYTDLGFKGLIGKELVAEEGTE
                     EEEEKPLQVSYAPGQVVTGKLGEKKEETKPPALYNDAAILSLMEKAHLLVSDKALREA
                     LKEASGIGTAATRAGFIPTLIAREYIKKEKQVYVPTPLGLELEPLLPEELKVPDYSAR
                     LEHHLWNMVKGKGSITFDQFMRESLEFLRRVFGKIQGSETRLNQLDAADSLGPCPKCG
                     KGHVLEREKCYACSDRNGCGFVLWKVYANAKLTKTELKNLLKNGETKKALKMKSKEGR
                     EFEARLKLNRESWKLDFLFEEKKSEAPAAPTSPALSAGQRPLSEKQIGVIERNAPKTV
                     KKAVAKGDYEAGRRWL"
     gene            complement(2399..3028)
                     /locus_tag="PKH10_08305"
     CDS             complement(2399..3028)
                     /locus_tag="PKH10_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28165.1"
                     /translation="MKGLFAMIAVSLIIACSTDGKYETCDGVEITPETARVQLLTTSG
                     SFYCKEMEEVFDIDAETYVKSFTGGVFLFTHATSLSSDSSTCMEMAVADIPVLYPANI
                     KIGALVKCLIGKAPRNETCADTFELTFATEDGGELMIQHRIDPQTLLGFAGFYKKDSL
                     SDPMTLLGTAFPACFENSDDGNIYVGNEFSLPQNATGTLLYKWISPAIL"
     gene            complement(3082..3375)
                     /locus_tag="PKH10_08310"
     CDS             complement(3082..3375)
                     /locus_tag="PKH10_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017865561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT28166.1"
                     /translation="MLKKDLVLKVAEKGGRSKRAAGIFVNFMFDAMINALLREERIAI
                     RNFGNFTVRHYGGYTSINPKSGERIAVPAKRLPYFRAGKDLLKRINHSTGGPA"
     gene            3758..>3956
                     /locus_tag="PKH10_08315"
     CDS             3758..>3956
                     /locus_tag="PKH10_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28167.1"
                     /translation="MKNGDPAILTALSFLSKLLNEEGLAVEQISPSSLHLPSSNTTIN
                     FLSLSYLIPSLSEDILALLRPH"
BASE COUNT          892 a         1171 c          916 g          977 t
ORIGIN      
        1 ccaaccacct cctccccgcc tcataatccc ccttcgcaac cgccttctta accgtctttg
       61 gcgcattcct ctctatcacc ccgatctgtt tctcggaaag cggtctctgc cccgctgaca
      121 gggcagggga ggtgggcgcc gccggcgcct ctgatttctt ctcctcaaag aggaaatcga
      181 gtttccacga ttcccgattc agtttgagcc gcgcctcaaa ctccctccct tccttactct
      241 tcatcttaag cgccttcttc gtctccccgt tcttaaggag attcttaagt tccgtcttcg
      301 tcaatttcgc attcgcatag accttccaca gtacgaaccc gcatccattc cgatcggaac
      361 acgcataaca cttctcccgt tccagcacat gccccttccc gcatttgggg caggggccga
      421 gcgagtcggc cgcatcaagt tgattaagcc gcgtctcact cccctgtatc ttcccgaata
      481 cccgccgcaa aaactcaagt gattccctca tgaactgatc gaaggtgata ctccctttcc
      541 ctttcaccat attccagaga tgatgttcca gacgggcgct gtagtcgggc accttaagtt
      601 cctcaggcag cagcggttcc aattcaagcc ccaacggtgt cggtacataa acctgtttct
      661 ccttcttaat atattcccgc gcgataagtg tcggtataaa accggcgcgt gtcgccgccg
      721 tcccgatacc tgatgcctcc ttaagcgcct cccggagcgc cttatcgctc actaaaaggt
      781 gcgccttctc catcagtgaa aggatcgccg catcgttata aagcgcaggc ggcttcgttt
      841 cctccttctt ctcccccaat ttcccggtga ccacctgtcc cggcgcataa gagacctgca
      901 gaggcttctc ctcttcctcc tcggttcctt cctcggcaac caattccttc ccgatcaacc
      961 ccttaaaccc gagatcagta taattcttga accgcgcaag gaacgggaac ccctctatct
     1021 ttcccaacat ctccgtcgta tcgcctttcg caggcggtaa aagcgccgca aggaaccgct
     1081 tcacgataag gtcaaatacc ttccgttcct cctcggaaag ggtcggtacc ccgttacgat
     1141 cataagaaag gaccggaata atggcataat gcccctgcaa ctcgccgatg tatttcttgg
     1201 cgagcgcaag atcaggattc atggtgcgta ccgtcgcaag gagttgccga taatcaggga
     1261 accgctccac gagcgcaacc gcctttccca gttccttcgc ctcctcgggc gacaggacat
     1321 tacagtcggt ccgtggatag gagatgagtt catgcgtctc atagagcgcc tgcgcgatct
     1381 tgagtgtgtc gtccggggtg aacttgtacc tgttcgcggc atctttctgg agttgcttga
     1441 ggtcgtagag ggcgggggca tattccgtga agggcttctt ctcaaccgcc gttatggcga
     1501 gcggcttacc ggcgatcgtt acgagtttct tctgctcttc cgcatcaacc gcgaagaaac
     1561 gccgtatctt cttctcttca tccacaaggt tgaagaggac accatcagtt gtcttaaacg
     1621 aataagtcca gaaagtttgt ggtttgtgtt ccttattctc acgccaccgt ttcacgatct
     1681 cagcaaggag ccatgactgt acacgcccca ccgatatgag atgacccggc ctgccgtatt
     1741 tcactgaata aagccgcgtc aactggagcc cgaccgtcca atcgaccgct tcccgtgtcc
     1801 gtgccgcctg ataatagccc tcatagtcag tatagggttt ccgttgttca aatgctttcc
     1861 gtatctcaga ggcggtctca ctatggagcc agacacgcgt gagcgtacgc gccttcagga
     1921 gaccacggtc agccgcgatc tccatgatat tccgatggat cagttctcct tcccgatcag
     1981 ggtcacacgc gagtactact tcctccgcct gtacgagatg tttcgctatc tccgcgaacg
     2041 cttcttcctc ccggatcaca tgtacgaatt tcgacggtac gaacggaaga tgcggtattg
     2101 acccgcgcca agtattattc tcacctaatg tatcggcaag atactggtta atatcggcga
     2161 gcgcctcaat atgaccgaag gaattggcga cccgcaagtc agggatcccc ttcaatgctt
     2221 cacgatactt cttcgccatt gaaggctttt cggtgatgat aagtttcaag cgggacctcc
     2281 ttaaaaaaaa ttggcaggtg ataattacac cggaagggga ggggagtcaa ggggaagggt
     2341 tcgttacctc tcgaatccgg cggacggctc catccctttt gatccttgct tgcctagcct
     2401 acaatatggc gggagatatc cacttataga ggagagtgcc ggtcgcgttt tgaggcaagg
     2461 aaaactcgtt tccaacatat atgttcccgt catcggaatt ctcgaaacag gcgggaaaag
     2521 cggtgcctaa aagcgtcatg gggtcggaaa gagaatcttt tttatagaag ccagcgaagc
     2581 ccaacaatgt ctgcgggtca atacggtgct gaatcatcaa ttcgcccccg tcttccgtgg
     2641 caaaggtcaa ttcaaaagta tcggcgcagg tctcgttccg aggtgccttg ccgatcaaac
     2701 attttacgag ggcgcctatt ttgatattgg caggataaag aacaggaatg tcggcaactg
     2761 ccatttccat acaggtagag gagtcggaag aaagcgaagt ggcgtgagtg aataaaaaaa
     2821 cgcctcccgt gaaactcttc acataggttt ccgcgtcgat atcgaagacc tcttccattt
     2881 ccttgcagta aaaagaaccg gatgtcgtca ataactgtac gcgtgccgtc tccggtgtta
     2941 tttcgactcc gtcacaggtt tcatatttgc cgtccgtcga acaggctatg atgagcgaaa
     3001 cagcaatcat agcaaatagc cctttcatga taccctccat ctcatgttat tcatcaagaa
     3061 acacttcggt gatgatgagt ttcaagcggg gcctcctgtg gaatgattga tgcgtttcag
     3121 aagatctttc ccggcacgga aataggggag ccgcttggcg ggaaccgcta tccgttcgcc
     3181 gctcttcgga ttgatactgg tatagccgcc ataatgccgc accgtaaaat taccgaaatt
     3241 ccggatagcg atccgttctt cccgcaggag cgcgtttatc atggcatcga acatgaagtt
     3301 cacgaatatc ccggccgccc tcttcgagcg ccctcccttt tcggcaactt tcaacacgag
     3361 atcctttttc agcatacacc cctccctctt ccacccgtct ctcgggtcaa tgaacgataa
     3421 ttacaccgga aggggagggg agtcaagggg aatgatccgg cgtccctgcg atccgcggag
     3481 gtcctgtggt tgaaacggtt cggccaacgg ccaacggcag cgaccagcga actccggctt
     3541 gttccgccat cgtgtccggc gaagttagac gaagccggaa gcccggagca aaggcggaag
     3601 tccgctgtca agggccgttt cgccctactc caagtcatca taattcataa gtaatattga
     3661 tatgtttctg tttttccact cccaaaatca accttttcat taccctatgg gcaacattta
     3721 tatttacaaa tgctgcccat ctgcatataa tggacccatg aagaacggag accccgccat
     3781 cttaaccgcc ttatccttcc tgagcaagct gctcaacgag gaagggctcg ctgttgaaca
     3841 gatatccccc tcatctctcc atctcccttc gtcgaacacc accatcaact tcctttcctt
     3901 gtcatacctg atcccttccc tgagcgagga tatcctcgcg ctcctccggc cccatc
//
LOCUS       DAOQUE010000255         3910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_26161_length_3910_cov_4.516213, whole genome shotgun sequence.
ACCESSION   DAOQUE010000255 DAOQUE010000000
VERSION     DAOQUE010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26161_length_3910_cov_4.516213"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKH10_08320"
     CDS             complement(<1..469)
                     /locus_tag="PKH10_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNT28168.1"
                     /translation="MTVLFEDIKVTEQTIADTFKDLSRRCQRGGGALKEQPGTVREIE
                     RRALGFLQGVSDRPAPLHLLLAILGDKQSIAYQILQEIGLQNELRLRAMNTLTNPPRR
                     MRNRINELFAETEEFLPAATPQARTRVKEEVLVAPETSEVPASAERPVAAASQA"
     gene            complement(662..2626)
                     /locus_tag="PKH10_08325"
     CDS             complement(662..2626)
                     /locus_tag="PKH10_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNT28169.1"
                     /translation="MSLCYRCMEDRGSEPVCIKCGFDSRSMGENTGPQLSPGTPLAEG
                     KYLLGRVLGIGGFGITYLGYDTRLKIKVAIKEYFPRHIAGRALPDPHVRPHDDQAGID
                     FKDGLRYFLREAQTAAKFDSHPHVVAVKDYFEEHGTAYIVMAYIEGRSLGDHIDKHPF
                     GRLSYQKTLEVMLPVFDALEKIHAANLLHRDISPENIYLTKDGTVKLIDFGAARYTFT
                     SQTQSMSLTLKEGYAPVEQYSKNGEQGPWTDIYAAAATIYRALTGKVPPAALERLHND
                     ELKPPSKLGAIIPAQAEKALLKALSLRIRDRYQDIAAFRKDLVAAMPRSGVAAFFADR
                     RVVAATLATLLILFAGTTFFLITKERTDERIPEEALRFIGKDADGNPLQPEQKETERV
                     RKPRAVKKHAAAPAKAPATAPQKKAEPSASRMIVKEEPEEEEQPPAETPAPVEDPARG
                     RLRKVQDKNEVSFVKAYVPQGEPADLFKQALALERAGNRRKALEYLEAACDQRYSIAC
                     YQEAKLSGDKRRYAVARQLFEGECAAGKGFACTNYGTMLANGEGGAKDISKARDILER
                     SCKLDQAVACLSLADMMAKGLGSVVDDPAMAAKFYKRACDLNSKEGCGRYADVLASGA
                     SGQKDGEKAAKYYYLRACQLGLSSACKKSK"
     gene            complement(2850..3773)
                     /gene="cysK"
                     /locus_tag="PKH10_08330"
     CDS             complement(2850..3773)
                     /gene="cysK"
                     /locus_tag="PKH10_08330"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNT28170.1"
                     /translation="MPIHQNVLELIGGTPLVRLNRMSAETGATVCAKLEYFNPMHSVK
                     DRIAFGMIEAAEEAGTIKPGATLIEPTSGNTGVGLALVAAVKGYRLILTMPETMSMER
                     RKLLKALGAELVLTPGPEGMSGSIAKAEELQRSTPGAVILQQFENPANPAFHRKTTAE
                     EIWRDTDGKVDIFVAGAGTGGTVTGVGSALKKKNAAVTVVTVEPDESPVLSGGTAAPH
                     KIQGMGPNFLPKVFDRTVVDEILRASADDAFAEARRAAKEEGIFCGISSGANLWAART
                     VAMRPENKGKLIVTVICDTGERYLSTPLFEE"
     gene            complement(3775..3906)
                     /gene="thiS"
                     /locus_tag="PKH10_08335"
     CDS             complement(3775..3906)
                     /gene="thiS"
                     /locus_tag="PKH10_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNT28171.1"
                     /translation="MKLDEAQFLAVSRNGTVVKRGDWTATALAAGDTIDILTIVGGG"
BASE COUNT          636 a         1227 c         1223 g          824 t
ORIGIN      
        1 cggcctgcga ggccgccgcg acggggcgtt cagcggaagc ggggacctcg gaggtctccg
       61 gcgcgacgag cacctcctcc ttgacccggg tccgcgcctg cggcgtggcg gcgggaagga
      121 actcctcggt ctcggcgaac agttcgttga tacggttcct catgcggcgc ggcggattgg
      181 tgagcgtgtt catggcgcgg aggcggagtt cgttctggag cccgatctcc tgcagtatct
      241 ggtaggcgat gctctgcttg tcacccagta tcgcgaggag caggtggagc ggcgccggcc
      301 tgtcggacac gccctgcagg aacccgagcg cacgccgctc tatctccctc acggtgccgg
      361 gttgttcctt gagcgcaccg ccgccgcgtt gacatcgccg ggagagatcc ttgaaggtgt
      421 cggctatcgt ctgctcggtc accttgatgt cctcgaacag gaccgtcacc tcgctcggta
      481 tcgtgaagaa agagagaagg aaatgaaaac tggagagatc ctcgtcgaga ctttgcgcga
      541 aatcgagcgc gtcgccacag cgccgcgagg gtatcggggg cctgcgcctc catcggtgct
      601 cctctcttgc ctaggaccgg gtccggtgcc attctagcga cgccgttccc tttttcaaca
      661 tttacttgga ctttttacag gcgctcgaca acccgagttg gcaggcgcgc agatagtagt
      721 atttcgccgc tttctcgccg tccttctgtc ccgacgcgcc gcttgcgagc acatcggcgt
      781 agcgaccgca tccttccttc gagttcaggt cgcaggcccg tttgtagaat ttcgccgcca
      841 tggccggatc gtccaccacc gaaccgagac ccttcgccat catgtcggca agagaaaggc
      901 aggcgaccgc ctgatccagt ttacaggacc gttccagtat gtcgcgcgcc ttcgagatgt
      961 ccttcgcgcc gccctcacca ttggcaagca tggtcccgta gttggtgcag gcgaaccctt
     1021 tgcccgccgc gcactccccc tcgaacagtt gacgggccac ggcgtatctt cgtttgtcgc
     1081 ccgacaattt ggcctcctga taacaggcta tagaatacct ctggtcacac gccgcctcga
     1141 ggtattcgag cgccttgcgc cggttccctg ctctctcaag cgccaaggcc tgcttgaaga
     1201 ggtcagccgg ctcgccctgc gggacatagg ccttgacgaa cgagacctcg ttcttgtcct
     1261 gcaccttgcg aagtctgccg cgcgccggat cctcgaccgg tgcaggggtt tcggcggggg
     1321 gctgttcttc ctcctcgggc tcctccttca cgatcatccg cgaagcggag ggctcggctt
     1381 ttttctgcgg ggcggtggca ggcgctttcg ccggagcggc cgcatgtttc ttcacggcgc
     1441 gcggcttccg tacccgttcg gtctctttct gctccggttg aagcggattg ccgtcggcat
     1501 ccttgccgat gaagcgaagc gcctcttcgg gtatccgctc gtctgtgcgt tccttggtga
     1561 tgaggaaaaa ggtcgttccg gcaaagagaa tgagaagcgt cgcgagcgtc gcggcgacga
     1621 cccgccggtc ggcaaagaag gccgcgaccc ctgagcgggg catcgcggcg acgaggtcct
     1681 tgcggaacgc ggcgatgtcc tgataacgat ccctgatacg cagggagagc gccttcagca
     1741 gcgccttttc ggcctgcgcc ggtatgatcg cgccgagttt cgacggcggc ttgagttcgt
     1801 cgttatggag ccgttcgagc gcggcgggag gcaccttgcc ggtcaacgcg cggtatatcg
     1861 tcgctgcggc cgcgtagatg tcggtccacg gtccctgttc gccattcttg ctgtactgtt
     1921 cgaccggcgc gtatccctcc ttgagcgtca ggctcatcga ctgcgtctga ctcgtgaagg
     1981 tgtagcgcgc cgcgccgaaa tctatcagtt ttaccgtgcc gtccttggtc aggtagatgt
     2041 tctcggggct gatgtcgcgg tgcaggaggt tggccgcgtg tatcttctcg agcgcgtcga
     2101 acaccgggag catcacctca agcgtcttct gataggaaag gcggccgaac gggtgcttgt
     2161 cgatgtgatc gccgagggag cggccttcta tgtaggccat cacgatgtag gcggtgccat
     2221 gctcctcgaa gtaatccttg accgcgacca catgcgggtg gctgtcgaac ttcgccgccg
     2281 tctgcgcctc gcgcaggaag tagcgcaggc cgtccttgaa atcgatcccc gcctgatcgt
     2341 cgtgcggtct cacgtgcgga tcgggaaggg cgcggccggc gatgtgacgc ggaaaatatt
     2401 cctttatcgc cacttttatc ttgagccgcg tatcgtagcc gagataggtg atgccgaaac
     2461 cgccgatgcc gagcacgcga ccgagcaggt atttgccctc ggcgagcggg gtaccgggcg
     2521 acagttgcgg accggtgttc tctcccatcg agcgcgaatc gaagccgcac ttgatgcaga
     2581 ccggttccga accgcgatct tccatgcaac gataacagag actcacggca cctccaagac
     2641 acatgccgct agactatatc ataagaatag ggaaaagcaa acggcctgtg gcttccccct
     2701 gtcaaggggg attgaggggg ttttgtccct tcacgatgcg agaaaaccct ccctgccctt
     2761 acctcgcatg aatgctgcgg cttccggcct gacgacactg tactacagtc tcgtccgaga
     2821 cggcctacag tgacagggcg ggaggatcgt cattcctcga acagcggtgt cgaaagatag
     2881 cgttcgccgg tatcgcagat gacggtgacg atgagtttgc ctttgttctc gggccgcatt
     2941 gccacggtac gcgccgccca gaggttggcc cccgaactga tgccgcagaa gatcccttcc
     3001 tcctttgccg cccgacgcgc ctcggcgaag gcgtcgtcgg ccgacgcacg gagtatctcg
     3061 tcgacgaccg tgcggtcaaa cacttttgga aggaagttgg ggcccattcc ctgtatcttg
     3121 tgcggcgccg ctgtgccgcc cgagaggaca gggctttcgt ccggttcgac cgtgacaacg
     3181 gtgaccgccg cgttcttctt tttcagcgcg gagccgacgc cggtaacggt gccgccggta
     3241 ccggcccccg cgacgaagat atcgaccttc ccgtcggtgt cgcgccagat ctcctcggcg
     3301 gtcgtcttgc ggtggaacgc cggattggcc gggttctcga actgctggag tatcaccgcg
     3361 ccgggggtcg atctttgcag ttcctcggcc ttcgctatcg agccgctcat cccttcgggg
     3421 ccgggggtga gaacgagttc cgcgccgagc gccttcaaaa gtttgcgccg ttccatcgac
     3481 atcgtctcgg gcatcgtgag gatgagtcgg tagcccttca ccgccgcgac gagcgcgagt
     3541 ccgacgccgg tgttgccgct cgtcggttcg atgagcgtcg cgcccggttt gatggtcccc
     3601 gcttcctccg ccgcctcgat catcccgaaa gcgatgcggt ccttgaccga gtgcatcgga
     3661 ttgaaatatt ccagtttcgc gcagacggtc gccccggtct cagcgctcat ccggttgagg
     3721 cgcaccagcg gtgtgccgcc gataagttcc agtacatttt gatgaatggg catcctatcc
     3781 tcctcccaca atggtcaaga tgtcgatggt gtcgcccgcc gcgagcgcgg tggcggtcca
     3841 gtcgccccgt ttgacgaccg tgccgttgcg cgacacggcg aggaactgcg cctcgtcgag
     3901 tttcatggtc
//
LOCUS       DAOQUE010000256         3872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_26501_length_3872_cov_4.210637, whole genome shotgun sequence.
ACCESSION   DAOQUE010000256 DAOQUE010000000
VERSION     DAOQUE010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26501_length_3872_cov_4.210637"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="PKH10_08340"
     CDS             complement(<1..2022)
                     /locus_tag="PKH10_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT28172.1"
                     /translation="MQRRIFVHLISLAFLAIAGITAIAAYHIRTGTIRYHVETFSKVA
                     EEVHHNMGRLLAGAVSDVALISNNPLFRGGATDPKTMEAALHDIHKILPRYDDISLIA
                     PNGTVIASTSYRFLADWRHKKHFQASLRGAVAVSPVSVSSEPFRQFLQFTSPVSQNGT
                     VKAVISLQLDLAKIAEIPLGVHIGETGNGFVTDHTGNIIIHHDPRRILEPMPVEFASA
                     GQGDGVFFTENGSQFFGNMFAPSPEDTIFPAEIPPWRVVIAERSEELLSAARLAILQA
                     ILFAAAIFVVAVLVSLQLSRRIVRPLGDLTTAMADISGTELSVSLPPPTNDEIGALSR
                     TFLRLLEDLRESRALLVSSEKRYREMADLLPLPLFEADLSGKIRYVNRVAFETFGYSE
                     EDIARGVSMFDVLVPEDRPRAMKNVEGRLNGTLVNRIEYTARRKDGTTLPVMVYNAPF
                     HEHERVAGLRGVVIDLSPIREAEEELSHKEQQLMQAQKMESIGTLVGGLAHDFNNVLG
                     GIGATSSLLTYMNEHDPSAVTPDKLRESLGLIDDQVKRASEMIRKLLTLSRRQKISFL
                     PMDLVRSVQNVAIIAEKTLDKSVRLRLSLPATPALINGDGALLEQVILNLVLNAWHAM
                     TIMRREGGTPGGVCAITLERTDIGEGFPNWPPGLSSGPYWSLRVTDTGVG"
     gene            complement(2036..3082)
                     /locus_tag="PKH10_08345"
     CDS             complement(2036..3082)
                     /locus_tag="PKH10_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNT28173.1"
                     /translation="MRRSVIFIAILCVFALLVLYFFFHSPSKTDRKEQELAILTWEDY
                     TDRQMVADFERETGIAVTVVEIKTSSEQVARLQDEPAAFDLVVVDESVLEYVLNAKLT
                     RPFDLSKIPNITDITSRFGTRLPMAVPQFWGTAGYIIDTRQVPADTDSVEMLWDPRYR
                     DRIRLLDDAPDAIVPLLHRARLSINGTASAEQENAIIRYAEELRENGVSFGDTFDNIA
                     KVMDGTVWIAQTYSGDYLYKARDLPHLRFVLPREGFRLWADYFVLSKSARNLEAAHRF
                     ISFRLRPEVAARASSTFFYPCAVKGSEKLLRPELRENSVMFPAISTLERGERYERNKD
                     PIPLHQKLFNLMKK"
     gene            complement(3180..>3872)
                     /locus_tag="PKH10_08350"
     CDS             complement(3180..>3872)
                     /locus_tag="PKH10_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT28174.1"
                     /translation="FKGTDKIGTSDYAAEKKVVAMNTPGQNSNAVAELAFGMMLYAAR
                     TWFTPKSGTELRGKKLGIHAYGFVGRSLAAIAKGFGMEIYAYDPFIDTATIAKDGVTP
                     VGSAEELYRTCQYVSLHIPATDKTKKSINHALLSLMPKGAVLINTARKEVIDEASLLK
                     MFEERPDFKYLSDVEDTVKDQVLEKYKDRAFFTPKKMGAQTEEANINAGIAAARQIVA
                     FFEKGDLTFKVN"
BASE COUNT          745 a         1056 c         1266 g          805 t
ORIGIN      
        1 gccgacgccg gtgtcggtga cgcggagcga ccagtagggg ccggaggaaa ggcccggcgg
       61 ccagttcggg aacccttcgc cgatgtctgt gcgttccaat gttatggcgc aaacgccgcc
      121 tggcgttccc ccttcccggc gcatgatggt catggcgtgc catgcgttca ggacgaggtt
      181 caggatgacc tgttcgagga gcgccccgtc gccgttgatg agcgccggcg tggcggggag
      241 cgacaggcgc agtcgcacgc tcttgtcgag cgtcttttcg gcgatgatcg cgacattctg
      301 gaccgaccgg acaaggtcca taggcaagaa ggagatcttc tggcgtctcg agagggtgag
      361 cagttttcgt atcatctcgg aggcgcgctt cacctgatcg tcgatcaggc cgagcgactc
      421 gcgcagttta tctggggtca ccgccgacgg gtcgtgctcg ttcatgtacg tgaggaggga
      481 agaggtggcg ccgatgccgc ccagcacatt gttgaaatcg tgggcgagtc cgccgacgag
      541 tgtgccgatg gactccatct tctgagcctg catgagttgc tgttccttgt gggacagttc
      601 ctcctcagcc tcgcgtatgg gcgagagatc gatcacgacg cccctgagtc ccgcgacccg
      661 ttcgtgttcg tggaacggcg cgttatagac cattaccggc agggtggtcc catccttccg
      721 gcgcgcagtg tattcgatgc ggttcaccag cgtcccgttc aggcggccct cgacattctt
      781 catggcacgc ggccgatctt ccggcaccag cacatcgaac atcgaaacgc cccgtgcgat
      841 gtcctcttcg ctgtacccga aggtctcgaa ggcgacacgg ttcacatagc ggatcttccc
      901 gctcaggtcg gcctcgaaaa gcgggagcgg cagaagatcg gccatctcac ggtaccgttt
      961 ctcgctggaa accagcaatg cgcggctttc ccggagatcc tccagcagac gcaggaaggt
     1021 tcgcgacaac gcgccgatct catcgttcgt cggcgggggc agggatacgg agagttcggt
     1081 ccccgatatg tcggccatcg cggtggtcag atccccgagc ggtcgcacga tgcggcgcga
     1141 gagttggagc gacacgagga cggcaacgac gaatatggcg gcagcgaaca ggatggcctg
     1201 cagtatggcc aatcgcgccg ccgagaggag ttcctcgctc cgttcggcga tgaccacccg
     1261 ccacggaggt atctcggcgg ggaagatggt gtcttctggc gacggcgcga acatgtttcc
     1321 gaagaactgg gagccgttct cggtgaaaaa gacgccgtca ccctgtcccg ccgaggcgaa
     1381 ctcgacaggc atcggttcga gtatccgtcg ggggtcgtgg tgtatgatga tattgcctgt
     1441 atgatcggtg acgaaaccgt ttccggtctc gccgatgtgc acgccaagcg ggatctcggc
     1501 gattttcgcg agatcgagtt gcagggagat gaccgccttc acggtcccgt tctgcgatac
     1561 cggtgaggtg aactggagga attgccgaaa gggttcggaa gaaacgctga ccggtgatac
     1621 ggcaacggcg ccccggagcg atgcctggaa atgttttttg tgacgccaat cggcgaggaa
     1681 tcggtaggat gtcgaggcga tgacggtacc gttcggcgcg atgagactga tgtcatcgta
     1741 ccgcgggagt atcttgtgga tgtcgtgaag tgccgcttcc atcgtcttgg gatctgtcgc
     1801 gccgccgcgg aagagggggt tgttcgatat gagcgcgaca tcgctgacgg cgccggcgag
     1861 caggcgtccc atgttgtggt ggacctcttc ggctaccttg gagaaggtct cgacgtgata
     1921 gcggatggtc ccggtgcgga tatggtaggc ggcgatagcg gttatgccgg ctatcgcaag
     1981 gaacgctaag gagataagat gaacgaatat acgccgttgc acgcgatacc tgccgctatt
     2041 tcttcatgag attgaacagt ttctggtgga gcggtatcgg atctttgttc cgctcgtagc
     2101 gttcaccgcg ttcgagggtc gatatggcgg ggaacatgac gctgttctcg cgcagttcgg
     2161 ggcgcagaag tttttcggag cccttgaccg cgcagggata aaaaaaggtg ctcgatgcac
     2221 gggcggccac ctcgggacgg aggcggaagg agatgaaccg gtgcgccgcc tccagattgc
     2281 gcgccgactt ggagagcacg aaataatcgg cccagagacg gaacccttca cgcggcaaca
     2341 caaagcgaag gtgcgggagg tcccgcgctt tgtaaagata gtcaccgctg taggtctggg
     2401 cgatccagac ggtgccgtcc atcaccttgg ctatgttgtc gaaggtgtcg ccgaaagata
     2461 cgccgttctc gcgtaactct tcggcataac ggatgatcgc gttctcttgt tccgcgctcg
     2521 cggtgccgtt tatcgaaagg cgggcgcggt gcagaagcgg cacgatcgcg tcgggggcgt
     2581 cgtcaagaag gcgtatcctg tcgcgatacc tcgggtccca aagcatctcg acgctgtcgg
     2641 tgtccgccgg gacctgtcgg gtgtcgatga tgtacccggc ggtgccccag aactgcggca
     2701 ccgccatcgg gagtctcgtg ccgaatcgag aggtgatgtc tgtgatgttg ggtatctttg
     2761 agagatcgaa ggggcgtgta agtttggcat tgagcacata ttcaagcacc gattcatcga
     2821 cgaccacgag atcgaacgcc gccggttcgt cctgcaggcg ggcgacctgt tcgctcgatg
     2881 tctttatctc gacgacggtc acggcgatgc cggtctcccg ctcgaaatcg gcgaccatct
     2941 gccggtcggt gtaatcctcc caggtcagga tggccagttc ctgctctttc cggtcggtct
     3001 ttgaggggga atggaagaag aaataaagca cgagcaacgc gaacacgcaa aggatagcga
     3061 taaaaataac cgagcgtctc actgccaacc ccttcatgag gatccaattg taacggggga
     3121 aaagggcaag gtcaagaaaa aagaacggaa cggttacaaa taaaaagagt aagcgggggt
     3181 caattcacct tgaaagtaag gtcgcccttc tcgaaaaagg cgacgatctg ccgggccgcc
     3241 gcgatgccgg cgttgatgtt cgcctcctcg gtctgggccc ccatcttctt cggggtgaag
     3301 aacgcgcggt ccttgtattt ttcgagtacc tgatccttga cggtgtcctc gacatctgag
     3361 agatacttga aatcgggccg ttcctcgaac atcttgagaa gggatgcctc gtcaatgacc
     3421 tctttacgag cggtgttgat gaggaccgct cccttgggca tcagcgaaag gagcgcatgg
     3481 ttgatgctct ttttggtctt gtcggtcgcc gggatgtgta gcgacacata ctgacaggtg
     3541 cggtacagtt cctcggcgct accgaccggg gtcacgccgt ccttggctat cgtcgcggtg
     3601 tctatgaacg ggtcgtaagc gtagatctcc atgccgaagc ccttcgcgat ggccgcgagc
     3661 gatcggccga cgaagccgta ggcgtggatg cccagtttct tgccgcgcag ttcagtgccg
     3721 cttttcgggg tgaaccaggt gcgggcggcg tagagcatca tgccgaaggc gagttcggcc
     3781 acggcgttcg agttctggcc gggagtgttc attgcgacga ccttcttctc ggcggcgtaa
     3841 tcgctggtgc cgatcttgtc ggtgcccttg aa
//
LOCUS       DAOQUE010000257         3858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_26636_length_3858_cov_3.814620, whole genome shotgun sequence.
ACCESSION   DAOQUE010000257 DAOQUE010000000
VERSION     DAOQUE010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26636_length_3858_cov_3.814620"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PKH10_08355"
     CDS             complement(<1..344)
                     /locus_tag="PKH10_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNT28175.1"
                     /translation="MSALTILAIGKLKKNGGFADMDRFYRDRIEPFAKLDIIELKESA
                     SVADDTAQLLRRIPAGSYVVALREEGRRLDSRAFAALLGQQRDAGRSIAFVVGGAYGF
                     GNVGEQIALSLAP"
     gene            complement(341..709)
                     /gene="rsfS"
                     /locus_tag="PKH10_08360"
     CDS             complement(341..709)
                     /gene="rsfS"
                     /locus_tag="PKH10_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNT28176.1"
                     /translation="MPETELLPKLRAIITALDEKKAVNTAVFSIEASSGYTDYMIVVT
                     GTSTQHNRTLADHTLRVLKEAGFSGASSEGEQHARWILVDAGSVIVHIMLDELRDYYD
                     LESLWPKAPRVDISAMIRQA"
     gene            complement(684..1625)
                     /locus_tag="PKH10_08365"
     CDS             complement(684..1625)
                     /locus_tag="PKH10_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28177.1"
                     /translation="MRPFPAALATIALLTVIAACSGRSDRPTESRWRFTDTVEYRPDI
                     NEGKLFVSLDETEKLLFAPLSFFEQHPSVIGFSQEISFLTESKAGNETRRLDLSDKVL
                     YTRDAANDFHLSYANNHSEGWDLVWKEGFLYKKLLGGDYVRTYSAGEHTFLKETLFRM
                     IPDLYEILRERAEIASTTEKGSLRHLTVRFVDRKMSREPLPPRRYLQNSFGAEEMNND
                     RIKTMLAGKDLTGVSGTLDIEVDRDRVIRRMVIDLAFTLSGEQVAFTVRGERTVTDKA
                     LLEVTVPPFSPEYHRRSFEAGKNIMEDPGHAGDRTAP"
     gene            complement(1622..2899)
                     /gene="hutI"
                     /locus_tag="PKH10_08370"
     CDS             complement(1622..2899)
                     /gene="hutI"
                     /locus_tag="PKH10_08370"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNT28178.1"
                     /translation="MRDTRAYTLILTDISELFTAAPQAPVLTGGPFCIAVDDRGIISF
                     VGRQADLSLREPDYETAFAAAVVSAGGRAVWPGLVDSHTHLVYAGTREEEFALKARGA
                     SYADIAARGGGILNSAKKTRAASADDLYHESRRRLLQVAAYGVTTIEMKSGYALDVAG
                     EIQILEVTARLARELPVTVVPTLLGAHAYPERFRNDHAGYISLLCDELMPEVKRRGLA
                     EFVDVFCEDGYFSLAETERILTRAKELGFGLKLHADQFHALGGTELAAGMGATSIDHL
                     EEVTDAAIRLMADNGTVAGVLPMTSVFSRLPFAPARRMADAGVRVALATDFNPGSCMC
                     GFLPLAASLGATQLSLSPEEAARGITLNGAASTGRERDRGSIEVGKAGDLIVMESRSW
                     LYPIYHMAHNHVRRIFIGGRPFEPAKMLAEEEA"
     gene            complement(2902..3198)
                     /gene="rpsT"
                     /locus_tag="PKH10_08375"
     CDS             complement(2902..3198)
                     /gene="rpsT"
                     /locus_tag="PKH10_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNT28179.1"
                     /translation="MAHHKSSKKRIKTSEKRNLYNRSMKSRIKTYIRHFSELLEKAAD
                     DVANLAQYKDEAETKFRKVVSELHKAVSKGVLQRSTAGRKISRLSAHLGKLFNA"
     gene            complement(3273..>3858)
                     /locus_tag="PKH10_08380"
     CDS             complement(3273..>3858)
                     /locus_tag="PKH10_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT28180.1"
                     /translation="LRENAPFRRFEAAFFTYGSGFMVVLPAMPILLVDHLNLSYSVVS
                     FGRGVMSAIFLIIFTPLLGRVLDRRDPVFLSMVSFFTLIAYPTLLIAAWFLRGAAEPL
                     FYLSFAAFGIAMAGVTLTWNVGPMYFAATPQEIPRLMSVHVTLTGLRGLLVPILGYFL
                     LKIGVLAPFVASAGFFFSAALMMRRQARRAPDKT"
BASE COUNT          697 a         1134 c         1288 g          739 t
ORIGIN      
        1 ggggcgagag aaagggcgat ctgttcgccg acattcccga aaccgtaggc gccgccaacg
       61 acgaaggcga tgctccgtcc cgcgtcgcgt tgttgcccga gtagtgcggc gaaggccctg
      121 ctgtcgagcc gcctgccctc ctcgcgcagc gcgacgacat aagacccggc ggggatgcgc
      181 cggagaagtt gggccgtatc atcggcgacg ctcgcgctct ccttcagttc gatgatgtcg
      241 agtttcgcga acggttcgat gcgatcacgg tagaaacgat ccatgtcggc gaagccgccg
      301 tttttcttga gtttgcctat cgcaaggatg gtcagggcgc tcatgcctgc cggatcatcg
      361 cgctgatgtc gacgcgcggc gccttgggcc agagcgattc gaggtcgtag tagtcgcgca
      421 gttcgtcgag catgatgtgt acgatcacgc tcccggcgtc caccagtatc cagcgggcgt
      481 gctgttcgcc ctccgaactg gcgcccgaga acccggcttc tttgagcacg cgtagggtgt
      541 ggtcggcgag cgtccggtta tgctgggtcg aggtgccggt gaccacgatc atgtagtcgg
      601 tgtagcccga tgacgcctcg atggagaaca ccgcggtgtt caccgccttt ttctcgtcga
      661 gcgccgtgat gatggcgcgc agtttaggga gcagttcggt ctccggcatg cccgggatcc
      721 tccatgatgt tcttgcctgc ctcgaaagaa cgccggtgat attcggggga gaagggaggc
      781 accgtcacct ccagaagtgc cttgtcggtc accgtccgct cgccgcggac ggtgaaggcg
      841 acctgttccc cggagagcgt gaaggcgaga tcgatgacca tgcgccgtat cacgcgatcg
      901 cggtccacct cgatatcgag tgtgccggac acgccggtca ggtccttacc cgcaagcatc
      961 gttttgatgc ggtcgttgtt catctcctcg gcgccgaagg agttctggag gtagcgtcga
     1021 ggagggagcg gttcgcgcga catcttgcgg tcgacgaacc ggacggtgag atggcgtaac
     1081 gatcctttct cggtggtcga tgcgatctcg gcccgttcgc gaagtatctc atagaggtcg
     1141 ggtatcatgc ggaagagggt ctccttgagg aaggtgtgct cgccggcgct ataggtccgc
     1201 acatagtcgc cgcccagcag tttcttgtag agaaaacctt ctttccagac gaggtcccag
     1261 ccctcgctgt ggttgttggc gtaggaaaga tggaagtcgt tcgccgcgtc gcgggtgtaa
     1321 agcaccttgt cggaaagatc gagccgccgc gtttcgttgc cggccttgct ttcggtgagg
     1381 aacgatatct cctgcgaaaa gccgataacg gaaggatgct gttcgaaaaa ggagagcggg
     1441 gcgaagagga gtttttcggt ctcgtcgagc gacacgaaga gtttgccctc gttgatgtcc
     1501 ggccggtact cgaccgtgtc ggtgaagcgc catcggctct cggtcggtct atccgatctc
     1561 ccggaacaag cggcgatgac cgtcaataag gcgatggtcg ccagcgcggc ggggaatggt
     1621 ctcatgcctc ctcctcggcg agcatcttcg ccggttcgaa cggtcgtccg ccgatgaata
     1681 tccgccggac atggttgtgg gccatatggt agatcggata aagccatgag cggctctcca
     1741 tcacgatgag gtcgcccgcc ttgccgacct cgatgctccc gcgatcccgt tcgcgtcccg
     1801 tcgatgccgc gccgttcagc gtgatgccgc gggccgcctc ctcgggcgag agcgacaact
     1861 gggtcgcacc gagactggcc gcgagcggca aaaaaccgca catgcacgag ccggggttga
     1921 aatcggtcgc gagcgccacg cgaacgccgg cgtcggccat ccggcgggcc ggggcgaagg
     1981 ggagccgcga gaaaaccgag gtcatcggga gtacccccgc gacggtgccg ttatcggcca
     2041 tgagccgtat cgccgcgtcg gtcacctcct cgagatggtc tatcgatgtc gcgcccatcc
     2101 cggcggcaag ttcggtcccg ccgagcgcgt ggaactggtc ggcgtgcagt ttgagcccga
     2161 agccgagctc cttggcgcgg gtgagtatcc gttcggtctc cgcgagtgag aaataaccat
     2221 cctcgcagaa cacatcgacg aactcggcga gtccgcggcg tttcacttcg ggcatcaatt
     2281 cgtcgcacag gagcgagatg tagcccgcgt ggtcgttcct gaaccgttcg gggtaggcat
     2341 gggcgccgag cagggtcggg accaccgtta ccggcagttc ccgcgcgagc cgggccgtca
     2401 cctcgagtat ctgtatctcg ccggccacat cgagcgcgta cccgcttttc atctcgatgg
     2461 tcgtgacgcc gtaggcggcg acctgcagga ggcgccgacg gctttcatgg tacagatcgt
     2521 ccgccgaggc ggcgcgggtc tttttcgccg agttgagtat gccgccgccg cgtgccgcga
     2581 tgtcggcgta tgatgcgcca cgcgccttca gggcgaactc ttcctccctc gtgccggcgt
     2641 agaccagatg ggtgtggctg tcgacgagtc ccggccagac ggcgcgaccg ccggcgctca
     2701 cgacggcggc ggcgaaggcg gtctcataat ccggctcgcg cagcgagaga tcggcctgcc
     2761 gtccgacgaa cgagatgatg ccgcggtcgt ccaccgctat gcagaacggg cctccggtga
     2821 gtaccggtgc ctgcggcgcg gcggtgaaca gttcgctgat gtcggtgagg atgagggtgt
     2881 aggcccgcgt atcgcgcacg atcaggcgtt gaacaacttg cctaaatgcg ccgaaagtct
     2941 gcttatcttg cgaccggcgg tggagcgctg gagcaccccc ttggataccg ccttgtgcag
     3001 ttccgatacc accttgcgga acttcgtctc cgcctcgtcc ttgtactggg cgaggttggc
     3061 gacatcgtcc gccgccttct cgagaagttc cgaaaagtgg cggatatagg tcttgatgcg
     3121 cgacttcatc gagcggttat acaggttccg tttctcgctc gttttgatgc gtttcttaga
     3181 tgacttgtga tgagccaaac cggtcctcct tctgtaacga tgttctcaag caccgaaaaa
     3241 cagtccttgt tatatgcgtg aaccgttccc tgtcaagttt tatcgggggc gcggcgggcc
     3301 tgccgccgca tcatgagcgc cgcgctgaaa aagaagccgg cgctcgcgac gaacggagca
     3361 agcaccccta tcttcaggag aaaatagccg agtatgggga cgagcaggcc gcgcaggccg
     3421 gtcagcgtca catggacgct catgaggcgc ggtatctcct gcggggtcgc cgcgaagtac
     3481 atcggcccga cattccaggt cagcgtcacg cccgccatcg cgatgccgaa ggcggcgaac
     3541 gacagataga agagcggctc ggccgctccg cgcaggaacc acgccgcgat gaggagcgtc
     3601 gggtaggcga tgagcgtgaa gaaggagacc atgctgagga ataccggatc gcgccggtcg
     3661 agcaccctgc cgaggagcgg ggtgaagatg atgaggaata tggctgacat cacgccgcgt
     3721 ccgaatgaga cgacactgta ggagaggttc aggtggtcga cgagcagtat gggcattgcg
     3781 gggaggacca ccatgaagcc ggagccgtag gtgaaaaagg cggcttcgaa acggcggaat
     3841 ggggcgttct cacgcagg
//
LOCUS       DAOQUE010000258         3855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_26671_length_3855_cov_3.600000, whole genome shotgun sequence.
ACCESSION   DAOQUE010000258 DAOQUE010000000
VERSION     DAOQUE010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26671_length_3855_cov_3.600000"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3855
                     /locus_tag="PKH10_08385"
     CDS             <1..>3855
                     /locus_tag="PKH10_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNT28181.1"
                     /translation="VVNAADTAGNELNDHNAGTLTIDRDMPGVVLTQATPTTINLSVN
                     ALTIQFEVNETPAADPILRIGADTIATIPVIEDITPGKRYTYTIPITHSESEGVKKAT
                     AEVYDAAGNHYYAELTYALGGSPDLIYDFTLPRVITRNANPERANVTTQGIQVLTSFS
                     EPVQKPAIATVQVQKRVGANWVTQSGMASRATVEDRPVGYTHALYFSLLDMLISNDGE
                     YRIHMQVTDENGNTAVTQGGDLLNLGEFRIDMTLPLIDATASVAAICTEPTDLNPGET
                     DYCSRPGHIDKTKAKAGDTIYYKLVFDRSDPYYEFDDIVDVWPTVEVSGLGAMAQLSQ
                     ITFAGDTAPYTFIYRRKITLTDTEDISSYALISMEDTAGNTYRKLSDDLVRTDVTKPT
                     LVAYSTTPSHAKVGTEIVVNFTLSEPVASVSFNGAGLAFAGGCTTTDNISYRCTHTVT
                     TGDTEGTKNFSATATDQTGNTSATLTLGSTVIDKTDPAVTITSFLISTVTTVNAMPAV
                     SHHQPDIGVSFQITPVSQFVGYPVVRFGGAEMSGLSCTGTTTYTCSGTYTVPSGETTE
                     GVKSVTVTVTDAAGNVTTQTPSSVLYDFTGPRLVSALFERTPNFAPSRDGATMIQHYS
                     IRDPYTNEFVTASLTLYADEPLAEGPTLNGFNLGTATVNYDEVYYTRALSDSDAEGLY
                     EIQVVWSDWLGNSVTRPIVWRLAIDKSEPASIDGEKVTFTRKPWGTDESGGLPSFSLA
                     AGIGTVIDTGIEMIQAIAPNGLLVGTATRQGDGSFSIDELTTSDVPVIYLNPVKKTGM
                     RIAYAPADPGATLEPARHVNWHATLNGKVPYSMLENPLVMVKTRRFMPHLDQGDEEPT
                     ASELQGVMSNDGTSFSHTSGYSWQRMDEGNHPLWRMGQQLLSEMVLDPNTGTVYSIGG
                     WYWTGAAPGYGNPTEYIDNLYTYDLTTDRANDPDPNGNKPPAQAHHGTLYDDINDVLV
                     VFGGKLYGPTNDLYFYYPEVNRWYLIEPTGTLPSARNHMGIAHDRKRDRYIIWGGSPD
                     NGTSGLLADMWEYDRVANEWSEVTQNGTIPSVRRGAKMVYDSRRHRLLLFGGQTTSNT
                     LLNDLYEFDIDSATWYTLSPTGTDVPLAQMGAQGAYDGRYQKFYIFGGTNDSAPTEGD
                     VKQHVYAYDIPRNEWSRIATTTGKIRMVYDPTNSRLVLIGRHYEDMTYLYQRQTVSLP
                     GGTSTTFTYGTNTPSARYGSTVVYDKNGNRTFMYGGANADGSLNELWQYDHASNQWTQ
                     LTNSGWPGLTARVFAAGTFDLNR"
BASE COUNT          868 a         1262 c         1064 g          661 t
ORIGIN      
        1 cgtcgtgaac gccgccgaca cggcgggcaa tgaactgaac gatcacaacg ccggcacgct
       61 caccatcgac cgcgacatgc cgggcgttgt tctgacgcag gcgaccccga cgacgatcaa
      121 cctgtcggtg aacgccctga cgatccagtt cgaagttaac gaaacacctg ccgccgaccc
      181 gatactccgc atcggcgccg acaccatcgc tacgatcccg gtcatcgagg atatcacccc
      241 cggcaagcgc tacacctaca ccattcccat aacacacagc gaatcggaag gggtcaagaa
      301 agcgaccgcc gaggtgtacg atgcggcggg caatcattat tacgccgaac tgacctacgc
      361 gctcggcgga tcgcccgacc tcatctacga cttcactcta ccgcgcgtca tcacgcgcaa
      421 tgcaaatccc gaacgggcca atgtcaccac acaagggata caggtgctca cctctttcag
      481 cgaaccggtc cagaaaccgg ccatcgccac cgtgcaggtg cagaaacggg tcggcgccaa
      541 ctgggtgacc cagtcaggca tggcctcccg cgccacggtc gaggaccgtc ccgtcggtta
      601 cacccatgcc ctctacttca gtctgctcga catgctcata agcaacgatg gcgaataccg
      661 tatccatatg caggtgaccg acgagaacgg caacacggca gtcacgcagg gcggcgatct
      721 gctgaacctc ggtgagttcc gtatagatat gaccctgccg ctcattgatg ccaccgcctc
      781 ggtcgccgcg atctgcaccg agccgaccga cctgaacccg ggcgagaccg attactgttc
      841 gcgtcccggt catatcgata agaccaaggc aaaggcaggc gataccatct actacaaatt
      901 ggtattcgac cggagcgatc cctactacga attcgacgat atcgtcgacg tctggccgac
      961 cgtcgaggtg tcgggattgg gcgccatggc tcaactttcg cagataacct ttgcgggcga
     1021 caccgccccc tacaccttca tttatcgtcg caagatcact ctcaccgaca ccgaggacat
     1081 cagcagttac gcgctcatct ccatggagga cacggcgggg aacacctacc gcaaactgag
     1141 cgacgacctc gtccggaccg atgtgaccaa accgacactc gtcgcctaca gcacgacgcc
     1201 gtcccatgcg aaagtgggta ccgagatcgt ggtgaacttc accctttccg agccggtcgc
     1261 aagcgtttcg ttcaacggtg cgggactggc cttcgctggt ggttgcacca cgaccgacaa
     1321 catcagctac cggtgcaccc ataccgtgac aacgggcgac accgagggga caaagaactt
     1381 cagcgcgacc gcgaccgatc agaccggcaa caccagcgcg acgctcacgc tgggcagtac
     1441 ggtgatcgac aagaccgatc cggcggtcac gatcacttcc ttcctgattt caacggtaac
     1501 aacggtcaac gccatgcctg ccgtttcaca ccaccaaccc gacatagggg tcagtttcca
     1561 gataacaccc gtctcccagt tcgtcggtta cccggtcgtg cgattcggcg gtgcggaaat
     1621 gagcggactt tcctgcaccg gcaccacgac ctacacctgt tccggcacct ataccgttcc
     1681 ctcgggcgag acgaccgaag gggtcaagtc ggtcaccgtg accgtgaccg atgccgccgg
     1741 caatgtcacg acccagacgc cgagctcggt cctctacgac ttcaccggac cgcggctcgt
     1801 cagcgcgcta ttcgagcgga cgccgaactt cgcaccgtcc cgggacggcg cgaccatgat
     1861 acagcactac agcatccgcg atccctacac caacgagttt gtgaccgcct ctctcaccct
     1921 ttatgccgat gagccgctgg ccgaaggacc gacgctgaac gggttcaatc tcggtaccgc
     1981 gacggtgaac tatgacgagg tctactacac ccgggcgctt tccgattccg acgccgaagg
     2041 actttatgag atacaggtcg tctggtccga ctggctcggg aacagcgtga cccgtccgat
     2101 cgtgtggcgg ctggcgatcg acaagagcga gccggcatcc atcgacggtg aaaaggtgac
     2161 ctttacccgt aagccatggg gcaccgacga gagtggcgga ttaccctcgt tctccctcgc
     2221 ggccggcatc ggcacggtca tcgacaccgg tatcgagatg atacaggcga tagcaccgaa
     2281 tgggctgttg gtggggacgg ctacacgaca gggcgatggc tcattctcca ttgacgaact
     2341 gaccacgagc gatgtgcccg tcatctatct caacccggtg aaaaagaccg gcatgcggat
     2401 cgcctatgcc cccgccgatc ccggcgcgac gctggagccg gcgcgccatg tcaactggca
     2461 cgccacgctc aatggcaagg tgccgtacag catgctggaa aaccccctcg tgatggtgaa
     2521 gacacgccgc ttcatgccgc atctcgatca gggcgacgag gaaccgaccg cctcggaact
     2581 acaaggggtc atgtcgaacg acggcacatc cttctcccat accagcggct acagttggca
     2641 acggatggat gaggggaatc atccgttgtg gcggatgggc cagcagttgc tcagtgaaat
     2701 ggtactcgac cccaacaccg gcaccgtgta ttcgatcggt gggtggtact ggaccggggc
     2761 ggcaccggga tacggcaatc ccacagagta catcgacaac ctctatacct atgatctgac
     2821 caccgaccgc gcgaacgacc cggatccgaa cggcaacaag ccgccggcgc aggcacatca
     2881 tggtacgctc tacgacgaca taaacgatgt gctggtcgtc ttcggtggta aactctacgg
     2941 cccgacgaac gatctctatt tctattaccc ggaggttaat cgctggtatc tcatcgagcc
     3001 gaccgggacc ctgccctcgg cccggaatca catggggatc gcgcacgaca ggaaacggga
     3061 ccgctacatc atctggggcg gctcccccga caacggtaca tcgggcctcc ttgccgacat
     3121 gtgggaatac gaccgcgtcg cgaacgagtg gtccgaggtg acgcagaacg gtacgatacc
     3181 ctcggtccgt cgcggcgcaa agatggtgta cgattcccga cgacaccgcc tgttgctgtt
     3241 cggcggccag acaacctcga acacgctcct gaacgatctc tacgaattcg acatcgactc
     3301 ggccacttgg tacaccctga gtccgaccgg aaccgatgtg ccgctcgcac agatgggcgc
     3361 gcagggggca tacgacggca gatatcagaa attctatatc ttcgggggca caaacgactc
     3421 tgcgccaacg gagggcgatg tcaaacagca tgtatatgcc tacgacatcc cacggaacga
     3481 gtggtcccgg atagccacga ccacgggcaa gatccgcatg gtctacgatc cgactaattc
     3541 ccgactggtg cttatcggtc gtcactacga ggacatgacc tatctctatc agcgccagac
     3601 cgtttctctg cccggcggca catcaacaac cttcacctac ggcacgaaca ccccatcggc
     3661 gcgctatgga tcgaccgtcg tgtacgacaa gaacgggaat cgcactttta tgtacggcgg
     3721 cgccaatgcg gacggatcgc tgaacgaact gtggcagtac gaccatgcgt cgaaccagtg
     3781 gacccaactg acaaacagcg ggtggccggg actgacggcc cgcgtattcg ccgcaggcac
     3841 cttcgacctg aaccg
//
LOCUS       DAOQUE010000259         3853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_26694_length_3853_cov_4.586361, whole genome shotgun sequence.
ACCESSION   DAOQUE010000259 DAOQUE010000000
VERSION     DAOQUE010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26694_length_3853_cov_4.586361"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PKH10_08390"
     CDS             complement(<1..486)
                     /locus_tag="PKH10_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28182.1"
                     /translation="MKYLVPLATGMIALLLAGACDNAKKTVMSDNDTLDTDSQTGADD
                     GAPPADIDTALTCGNGTVDTGETCDGGMIECVVIDAQAYKGGKAKCANDCSGWDTSTC
                     EEFPYTCGNGTVETGETCEKGDMTDCTDIDPDIYIAGKAYCRDDCSGWDTATCDISPD
                     AD"
     gene            complement(505..1731)
                     /locus_tag="PKH10_08395"
     CDS             complement(505..1731)
                     /locus_tag="PKH10_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28183.1"
                     /translation="MRMMFLMPAVFAALTVVIVVACAIEEEPSFIAGGWCDTPGVTAC
                     DDTFMTILYCNDAYLWEPLKECPEGCTEESGLATCIGGGSPDNAVPDTSDPQLPDTAD
                     EATGDDATITDATPLPDDTVGTDDGTVTDDDGEMTDDITPTDEDIVIDDTGTTDDATV
                     TDDATATDDATVADEDTVADEDTVTDDATASDDDVLLTIVVSYDFNDGAQGWTHATID
                     GYAGDTSWPYDFWEQGARNDGASCHEGTGCWGTNLDGNYINCQRAELRSPVIDLSAYA
                     GMTVTLYAWQSRDFWDNGTYFDGGILEFSGNGGSTWVNGCATCSGTVNIRTSSGIMYS
                     CNSNPFYVDDEKGFVQATGGWVSTYFSIPVEVLTANFMMRFVYSTGVSVGTTSSDPTD
                     YDHKGWYLDDVSIEAQ"
     gene            complement(1816..2046)
                     /locus_tag="PKH10_08400"
     CDS             complement(1816..2046)
                     /locus_tag="PKH10_08400"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HNT28184.1"
                     /translation="MTAHMRTVAEVFGVRIKVFNNGTDRPHFLAFYENFMGQFDLETL
                     EMTNGDLPSATITVVREWVADHIEELWEAWDL"
     gene            complement(2060..3814)
                     /locus_tag="PKH10_08405"
     CDS             complement(2060..3814)
                     /locus_tag="PKH10_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNT28185.1"
                     /translation="MKPRNLYELFSDNAARRPDHPAVETVTRTGVEHLSYGQFHGRVE
                     KLAGALAAAGVERGDRVILLSENRAEWYLALYAVLRAGGVAVPLYTTASVDQHAYIVR
                     DSEAKFAFVSKAALCGNLRGVADRLRGIVLFDDEPFDGAVPLDRFLAGGDPLFDPDRR
                     RPGDAEDPAVFIYTSGTTGEPKGVVLSHRNIISDVLMLQPVLDFIRGLRYLSILPLSH
                     AYEFTVIQTVFSMGGTVIPVPVMARTVEYSERGTPTIACAVPRLFEKIYHAVLHKVDM
                     GHPAARMLFYRGLRLGEKIYRFLEKNEPIPFPDNIAYRFYRRLVFDKIRGRTVRSIQL
                     FISGGAAIQPEIVRFFNIIGTPIVEGYGISECSPVVSVNMPDDRDVGTVDPLLPELDA
                     RLTTDGELLIRGPIVMQGYHNRPEETRAAMDGDGFFRTGDLAVWTDNRKIKIIGRKKE
                     IIVMASGKNVAPTKIENMIVADGWIEQACVVGDEQKHLAAILVPNFDALLPWAKEQGI
                     AIEDERELAHLPAVRKLVKQSIDRVNQRIESHEAIKRFHLHDRRFTVEGGELTPTLKL
                     RRREIQKRYRDTIEGLFR"
BASE COUNT          668 a         1138 c         1237 g          810 t
ORIGIN      
        1 gtcggcatca ggcgagatgt cgcaggtggc ggtatcccac cccgaacagt cgtctctgca
       61 atacgccttt ccagcgatat agatgtcggg gtcgatatcg gtgcagtcgg tcatgtcgcc
      121 tttttcgcag gtctcgcccg tctcaacggt gccattgccg caggtatagg ggaactcttc
      181 acaggtggat gtgtcccagc cagagcagtc gtttgcacat tttgccttgc cccccttgta
      241 tgcctgcgca tcgatcacga cgcactcgat catgccgccg tcacaggtct caccggtatc
      301 gacggtcccg ttgccgcagg tgagcgcggt gtcgatatcg gcgggcggcg cgccgtcatc
      361 ggcgccggtc tggctgtcgg tatccagcgt atcgttgtcc gacatcacgg tctttttcgc
      421 attgtcgcaa gctccggcaa gcagaagggc gatcattccg gttgcaaggg gaacaagata
      481 tttcatgcta tcctccgata ttgatcactg tgcttctatc gaaacatcgt caagatacca
      541 ccccttatga tcgtaatcgg tcggatcgga ggaggtggtg ccgacgctca cgccggtcga
      601 gtagacaaag cgcatcatga agttcgcggt aagtacttca acgggtatcg agaaataggt
      661 actgacccag ccgccggtcg cctggacaaa gcccttttcg tcgtcgacat aaaaggggtt
      721 agagttacag gagtacatga tcccggagga ggtgcggatg ttcacggtcc ccgagcaggt
      781 ggcgcaaccg ttgacccatg tcgagccgcc gttgcccgag aattcgagta tcccgccgtc
      841 gaagtaggtg ccgttgtccc agaaatcacg cgattgccat gcgtagagcg tcaccgtcat
      901 cccggcgtag gccgataggt cgatgacggg tgatcgtagt tcggcgcgct ggcagttgat
      961 gtagttcccg tcgagattgg tgccccagca gccggtcccc tcgtgacacg aggctccgtc
     1021 gttgcgggct ccctgttccc agaaatcgta gggccagctg gtatcaccgg cgtatccatc
     1081 gatggtggcg tgggtccagc cctgcgcgcc gtcgttgaag tcgtaggaaa cgacgatcgt
     1141 cagaaggaca tcgtcgtcgc ttgccgttgc atcatcggtg acggtgtctt catctgctac
     1201 ggtgtcttca tctgccacgg tggcgtcatc ggtcgccgtc gcatcgtcgg tgaccgtcgc
     1261 atcgtccgtc gttccggtat catcgatcac gatgtcttcg tcggtcgggg tgatatcatc
     1321 tgtcatctcg ccgtcatcgt cggtcaccgt gccgtcatcg gtcccgacgg tatcgtcggg
     1381 gaggggtgtt gcgtcggtga tggtggcatc gtcaccggtc gcctcatcgg ccgtgtcggg
     1441 cagttgggga tcgctggtgt cgggcaccgc gttgtcgggt gagccgccgc cgatgcaggt
     1501 ggcgagtccg ctttcctcgg tgcacccttc gggacactcc ttgagtggtt cccagaggta
     1561 tgcgtcgttg cagtacagga tggtcatgaa ggtgtcgtca caggcggtca ctcccggcgt
     1621 gtcgcaccag ccgccggcga tgaacgacgg ctcctcctcg atagcgcagg cgacgacgat
     1681 gacgaccgtg agcgccgcaa acaccgccgg catcaggaac atcattctca tggcggggac
     1741 cccctcttca gtatcctacg aacaccgtca ttgtagcgcg gaaacgcgtt ttagcaacag
     1801 aaaaaaggtc gccactcaca gatcccatgc ctcccagagt tcctcgatgt ggtcggcgac
     1861 ccattcccgc acgacggtta tcgtggccga agggagatcg ccgttcgtca tctcgagcgt
     1921 ctcgagatcg aactgtccca tgaagttctc gtaaaaggcg agaaaatggg gccggtccgt
     1981 gccgttgttg aacaccttga tcctgacgcc gaacacctcg gcgaccgtcc gcatgtgtgc
     2041 cgtcatcgct ccctcccgct caccggaaca atccttcgat ggtgtctcga tatcgtttct
     2101 gtatctcgcg tcgcctgagt ttgagcgtcg gggtgagttc gcccccctcc acggtgaaac
     2161 ggcgatcgtg gaggtggaat cgtttgatcg cctcgtggct ctcaatacgc tggttgaccc
     2221 ggtcgatgct ctgtttgacg agtttccgca cggcgggcag gtgcgccagt tcccgctcgt
     2281 cctcgatggc gataccctgt tccttcgccc acgggaggag cgcgtcgaag ttgggcacga
     2341 gtatcgcggc gagatgcttc tgttcgtcgc cgaccacgca cgcctgctct atccagccgt
     2401 cggcgacgat catgttctcg atcttcgttg gggcgacatt cttgccgctc gccatcacga
     2461 tgatctcctt tttgcggccg atgatcttta tcttgcggtt gtcggtccag acggcaaggt
     2521 cgccggtacg gaagaatccg tcgccgtcca tcgctgcacg ggtctcttcg gggcggttgt
     2581 ggtacccctg catgacgatg gggccccgga tgagcagttc gccgtcggtg gtcagtcgcg
     2641 cgtcgagttc gggcaggagc gggtcgacgg tgccgacatc gcggtcatcg ggcatattga
     2701 cgctcacgac cggcgagcat tcggatatcc cgtagccctc gacgatgggg gtgccgatga
     2761 tgttgaagaa gcggacgatc tcgggctgta tcgcggcgcc gcccgaaatg aacagttgta
     2821 tcgaccggac cgttcgtccg cgtatcttgt cgaatacgag ccgccggtag aaccggtagg
     2881 cgatattgtc ggggaacggt atcggctcgt tcttttcgag gaaccggtat atcttctcgc
     2941 cgagccgcag gccgcggtag aacagcatgc gtgccgccgg atgacccatg tcgaccttgt
     3001 gcaggacggc gtggtatatc ttttcgaaca gacgcgggac cgcacaggcg atggtggggg
     3061 tgccccgctc gctgtattcg acggtgcgcg ccatgaccgg gaccgggatg acggtgccgc
     3121 ccatcgagaa taccgtctgg atgacggtga actcgtaggc gtggctgagc ggcaggatgg
     3181 agaggtagcg caggccccgg atgaaatcga gcaccggctg gagcatgagc acatcgctga
     3241 tgatgttgcg gtgcgagagg acgaccccct tgggttcgcc ggtcgtcccg ctcgtgtaga
     3301 tgaacacggc ggggtcctcg gcgtcgccgg gacggcgccg gtcggggtcg aacagcgggt
     3361 cgccccccgc gaggaaacgg tcgagcggca ccgcgccgtc gaaaggctcg tcgtcgaaca
     3421 gcacgatgcc gcgcaggcgg tcggcgaccc cccgaagatt cccgcagagc gcggccttcg
     3481 agacgaaggc gaacttcgcc tcgctgtcgc ggacgatata tgcgtgctgg tcgacgctcg
     3541 cggtggtgta gagcggcacg gcgacgcccc cggcgcgcag gacggcatag agcgcaagat
     3601 accactcggc gcggttctcg gaaaggagta tgacgcggtc gcccctttcg acgcccgccg
     3661 cggcgagcgc gccggcgagt ttttcgaccc gcccgtggaa ctgaccgtag gataggtgtt
     3721 cgacaccggt gcgtgtcacg gtctcgaccg ccggatggtc gggccgtcgc gcggcgttat
     3781 ccgagaacag ttcgtagagg ttacgcggct tcatgcagat atccgttgac acggggtgat
     3841 aaagaggata cta
//
LOCUS       DAOQUE010000260         3848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_26740_length_3848_cov_3.346164, whole genome shotgun sequence.
ACCESSION   DAOQUE010000260 DAOQUE010000000
VERSION     DAOQUE010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26740_length_3848_cov_3.346164"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PKH10_08410"
     CDS             <1..347
                     /locus_tag="PKH10_08410"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNT28186.1"
                     /translation="AFPASNYGFDGTVAALTAISPEAKSIAVAIVSQRESWWYRLGQW
                     FFDQFKGMLLSDITLSPKYDKNCYPCSEMYEAFKPYEVDAVSGATYTSNAVTKDVWDA
                     FYLYDEVFTPPL"
     gene            420..1622
                     /gene="coaBC"
                     /locus_tag="PKH10_08415"
     CDS             420..1622
                     /gene="coaBC"
                     /locus_tag="PKH10_08415"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNT28187.1"
                     /translation="MQGKHITLAVTGAIAAYKAVHLLRLFREAGAEVQVIGTEHSLHF
                     VGRETWEALSGRPPLFGLWETPDPAKIPHIMLGQEVDLVVVAPATANMLAKAACGIAD
                     DLVSTVLLAATAPVLFAPCMNTAMWEHPATRRNVETLAARPGIHFVNCAEGKLACGTE
                     GKGRLAEPAVIFEMAGFLLTPKRPDRFRWLITAGATREYIDPVRFISNGFFGLMGRSI
                     ADAAWRLGGEVTLVTGPVADTTPRPYPVRTVTSAAEMATVVNEQAPLCDILVMSAAVA
                     DHAPVKSAAKQKKGGGTVTLTLHPTPDILTGTIPLMRPDALRVGFAAETEDLVKNARG
                     KLERKNLDLIVANLVSIDLDPLGSPINRVTIITTDAQEELPLMPKRELGALLAAKIHD
                     TAARKRRR"
     gene            1622..2296
                     /locus_tag="PKH10_08420"
     CDS             1622..2296
                     /locus_tag="PKH10_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT28188.1"
                     /translation="MEKLFSSGSVIVRRHEIAPFLMRIVSGKVSCEIPGRSWHLSDGD
                     FFGDEGVLLKKPAPYTAYAAEETVITMMNETEASAFLAGDPAAALAAVRRTAARLYEP
                     TGPLTPDNPLYLRLLKILLPHTQQQGGPDDFFPLTVTLDHLASSLDGTVESLRLLLKE
                     ATALDIVRVAEDDTIQAKSAEHLRRALSAGQSNRHPFSMETQRYGRGHFNLLSRIDPR
                     QEGQTP"
     gene            2293..>3848
                     /locus_tag="PKH10_08425"
     CDS             2293..>3848
                     /locus_tag="PKH10_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNT28189.1"
                     /translation="MKIACPSCSRQYTIDDQKIGPKGLLVKCSVCGAEFRAQRSAAVA
                     GSPPPSSVIPTASEESLDDIFNDILDKPADESKPLADGPKAPADTPKGQFDLDALHDK
                     YSSLLPGEEEPASMTIDKREERPVKRTEGAVIPSVDEPPAADPFIERNEDYVAATPGH
                     PPSGPTAGGGGEQKSAAPVEKDLLDSLFDDMGGDTSSDEEKTQNFNMLDLPLPSEEKK
                     TTAAPRERIEKKGAEKLARAPEGLSAEKKDALGDLFSDFSSENIEQRLLDKSSFGDAT
                     DTGDLPVGAAAEPMEKKIYIRRRQTNETIGPFPADQLSGMLLRKEINKNDFVSYDGVR
                     WEPIRDSGGAGGGGQTITASLKTDEPGLLNFDEDGRKAKLSLVESVASRQASPTSSAP
                     VHLFNDSIATFDDETMQRRDIEHTVIPDTGGPSLLDGTSPGTEDARPSKKKKKKKRGL
                     MVLLLKLMIVLVVVSVAAISAAWWYKNRRPKADILERISETITESAGTLSDVRESLEQ
                     DTRQGYLKSLG"
BASE COUNT          835 a         1259 c         1127 g          627 t
ORIGIN      
        1 acgccttccc cgccagcaac tacgggttcg acggtacggt cgcagccctc accgcgatct
       61 ccccggaggc gaaaagcatc gcggtcgcca tcgtttcgca acgcgagtcg tggtggtacc
      121 ggctgggaca gtggttcttc gatcagttca agggaatgct cctctcggac ataacgcttt
      181 cgccgaaata cgacaagaac tgctatccct gctccgagat gtacgaggcc ttcaagccct
      241 acgaggtgga tgccgtttcc ggagcgacct atacctcgaa cgccgtcacc aaggatgtct
      301 gggatgcgtt ctacctctat gacgaggtgt tcacccctcc cctctgaatt tttaggagcg
      361 ggaaaattgc caaatgccgg tggtccggtt atactccttc cgcaaaaaga gggaccggta
      421 tgcagggaaa gcacatcacg ctggcggtga ccggcgccat agcggcctac aaggccgttc
      481 atctgctccg actcttccgc gaggcgggag cggaggtaca ggtcatcggc accgaacatt
      541 cgctccattt cgtcggacgc gagacatggg aggcgctatc aggccgcccg ccgctcttcg
      601 ggctctggga aacaccggac cccgctaaga taccgcacat catgctcgga caggaggtcg
      661 atctggtggt ggtcgccccg gcgaccgcga acatgctcgc caaagcggcc tgcggcatcg
      721 ccgacgatct cgtctcgacg gtgcttctcg cggcgaccgc gccggtgctc ttcgctccct
      781 gcatgaacac cgcgatgtgg gaacacccgg cgacccgccg caatgtcgag acgcttgcgg
      841 cacggccggg catccacttt gtcaactgcg ccgagggaaa actggcctgc ggcaccgaag
      901 gaaaaggtcg actcgccgaa ccagccgtga tattcgagat ggccgggttc ctgctgaccc
      961 ccaagcggcc cgaccggttc cgctggctca tcacggctgg cgccacgcgg gaatacatcg
     1021 atccggtgag attcatatcg aacggttttt tcgggctcat ggggcgatcc atcgccgacg
     1081 ccgcatggcg gctcggcggt gaggtgacgc tggtgaccgg tcccgtggcc gatacgacac
     1141 cccgccccta cccggtccgt acggtgacga gcgccgccga gatggcgacg gtcgtgaacg
     1201 aacaggctcc gctctgcgac atactcgtga tgagcgccgc ggtcgccgac cacgcgccgg
     1261 taaaaagcgc cgccaagcag aaaaagggag gcggcacggt caccctgacg cttcatccga
     1321 cacccgatat cctgaccggt acgataccgc ttatgcgtcc cgacgccctg cgcgtcgggt
     1381 tcgccgccga gaccgaagac cttgtaaaga atgcccgcgg caaactggaa cggaagaacc
     1441 tcgatctcat cgtcgccaac cttgtctcga tcgacctgga cccgttgggt agcccgatca
     1501 atcgggtgac catcatcacc accgacgccc aggaggaact gccgctcatg cccaagagag
     1561 aactcggcgc gttgcttgcc gcgaagatac acgacacggc ggcgcggaaa cggaggcgct
     1621 gatggaaaag ctattctcct cgggcagcgt catcgtacgg aggcatgaga tagcgccctt
     1681 tctcatgcgt atcgtctcgg gcaaggtctc gtgcgagata ccgggccgtt cgtggcatct
     1741 ttcggacggg gacttcttcg gcgacgaggg agtactcctc aaaaaaccgg ccccctacac
     1801 cgcctacgcc gcagaggaaa cggtcatcac gatgatgaac gagaccgaag cgtcggcctt
     1861 tcttgccggc gatccggccg cggcgctggc cgccgtgcgc cggaccgcgg cacgcctcta
     1921 tgagccgacc gggccgctga cgcccgataa cccgttgtac ctccggctcc tcaagatact
     1981 cctgccgcac acgcagcaac agggaggtcc cgacgatttt tttccgctca ccgtaacgct
     2041 tgaccacctc gcatcgtccc tcgacgggac cgtggaaagc ctccggctcc tgctcaagga
     2101 ggcgaccgcg ctcgatatcg tcagggtcgc cgaggacgat accattcaag ccaagagcgc
     2161 cgaacacctg cgccgcgcac tttccgccgg acagagtaat cgacacccct tttccatgga
     2221 gacccagcgc tacggcagag gacatttcaa cctactaagc agaatagacc ctcgacagga
     2281 aggacagacg ccatgaagat cgcatgcccc tcgtgtagtc ggcagtacac tatcgacgac
     2341 cagaagatag gccccaaagg gctgctggtc aaatgctcgg tctgtggggc cgagttccgg
     2401 gcgcagaggt cggccgcggt cgccggatcg cctcctcctt caagcgtcat tcccaccgca
     2461 tccgaagagt cgctggacga catcttcaac gatatcctcg acaaacccgc cgatgagtcg
     2521 aaaccactcg ccgacggacc taaggcgccc gccgacacgc cgaagggaca gttcgatctt
     2581 gatgcgcttc acgataaata ctcgtccctg cttcccggcg aggaggagcc cgcgtcgatg
     2641 acgatagaca aacgggagga gcgaccggtc aagcggaccg aaggggcggt cattccctct
     2701 gttgatgagc cgccagccgc cgatcccttc atcgaacgca acgaagatta cgtcgcggcg
     2761 acacccggac atcctccttc gggacccaca gcgggcggcg gcggggaaca gaagagcgcg
     2821 gcacccgtcg aaaaggacct gctcgacagc ctcttcgacg acatgggagg cgacacctct
     2881 tccgacgaag agaagaccca gaacttcaat atgctcgacc tgcctcttcc ctcggaggag
     2941 aaaaaaacga ccgccgcacc gcgcgaacgc atcgaaaaga agggggcgga gaaactggcg
     3001 cgggcccccg aaggcctgag cgccgagaaa aaggatgcgc tcggcgacct tttcagcgac
     3061 ttctcttcag agaacatcga acagcggctt ctggacaaaa gttccttcgg cgacgccacc
     3121 gacaccggcg accttcctgt gggcgcggcc gccgaaccga tggaaaagaa gatatacatc
     3181 cgccgccgac agaccaacga aacgatcggc cctttccccg cggatcaact ttccggcatg
     3241 ctcctgcgca aggagataaa caagaatgat ttcgtgagtt atgatggtgt ccgatgggaa
     3301 cctatccgcg acagtggcgg cgccggcggc ggcggacaga ccatcaccgc ctcactcaag
     3361 accgacgaac cgggactcct caatttcgac gaggacggac ggaaagcgaa actctcgctc
     3421 gtcgaaagcg tcgcttcccg gcaggcctca ccaacaagca gcgcgccggt ccacctcttc
     3481 aacgattcca tcgccacctt cgacgacgag acgatgcaac ggcgggacat cgagcatacc
     3541 gtcatacccg acacgggcgg tcccagcctg ctcgacggca cctctcccgg caccgaggat
     3601 gcccggccct cgaagaaaaa gaaaaagaag aaacgagggc tcatggtgct cctgctcaaa
     3661 ctgatgatcg tgctggtcgt cgtttcggtc gccgcgatct ctgcggcgtg gtggtacaag
     3721 aaccgcagac cgaaggcgga catactggaa aggatcagcg agaccatcac cgagtcggcc
     3781 ggcacgctct ccgatgtgcg cgaatcgctt gaacaggaca cccggcaggg gtacctcaaa
     3841 agcctcgg
//
LOCUS       DAOQUE010000261         3843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_26796_length_3843_cov_4.396779, whole genome shotgun sequence.
ACCESSION   DAOQUE010000261 DAOQUE010000000
VERSION     DAOQUE010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26796_length_3843_cov_4.396779"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKH10_08430"
     CDS             <1..295
                     /locus_tag="PKH10_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28190.1"
                     /translation="TGMLDDAFSRRFLYKLEFPRPAPAIRALIWRSKLPELSEEEADA
                     LSAFDLSGGEIENVTRRYIVDTLFSGKPDLERLEVLCEQESSLKKGTGRKVGF"
     gene            complement(450..1352)
                     /locus_tag="PKH10_08435"
     CDS             complement(450..1352)
                     /locus_tag="PKH10_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNT28191.1"
                     /translation="MKPITNLAASVRQRLLNKAREGNRSFQEIAQSYLMERFLYRLSR
                     SSQKENFVLKGALMLRVWQAPEARATMDIDLLGRVSNEEAATAAAIRKVLASEVEPDG
                     LEFSAVDMVIERITEGAEYRGLRVQIPALLDTMRLHLQIDIGFGDPIYPPPQKENFPT
                     ILDLPAPVISCYSRESAIAEKLHAMLTHGQLNSRMKDFHDIWWLSRRFDFDGAVLLEA
                     IDRTLSKRGISVPDPIVAFSDQFADTHQVQWSAFIKRMSPSDVPASFALIVRAVALFL
                     GPLVEAHKKGTSFTDRWIAPGPWK"
     gene            complement(1349..1948)
                     /locus_tag="PKH10_08440"
     CDS             complement(1349..1948)
                     /locus_tag="PKH10_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNT28192.1"
                     /translation="MPADQKALKIIERTGGIIHTAEAIKKGIHPRTLYLLRDTGQLTT
                     VSRGIFRLADLPELTQPDLATVAARVPHAVICLVSALSFHNLTTQRPATVSIAIASSA
                     RSPRIDFPPLSVFRFSEESLSAGVEKHLFDGIAVRIFSPEKTIADCLKFRNKIGFDIF
                     AEALKLYRARRRFDVDALLAYGRTCRVERLMKPYLEALL"
     regulatory      2107..2181
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            2174..2437
                     /locus_tag="PKH10_08445"
     CDS             2174..2437
                     /locus_tag="PKH10_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28193.1"
                     /translation="MSQQFTPYVTRRLIAAIYQRIAVPPECVTEEEMITFTRATTQGH
                     FKCVLVPGPGRAIWFNEKGEITGRDETGAKLPEARPDVGRVVF"
     gene            2457..2666
                     /locus_tag="PKH10_08450"
     CDS             2457..2666
                     /locus_tag="PKH10_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28194.1"
                     /translation="MSRTDPAYFRTSEILKKMEKGLLTVEFTAKYLARFLKSGTLSRQ
                     DFLEFTQVDEIRTRYKTIEGEVGKI"
     gene            complement(2685..3215)
                     /locus_tag="PKH10_08455"
     CDS             complement(2685..3215)
                     /locus_tag="PKH10_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28195.1"
                     /translation="MDDKQLEFLRSVFLSNAQQGAFQRAHVYREGIDTKEFTKRRDDF
                     IKDLHAKLRMLEEPYRMVVDGEEHCRNIQRIAEELSSKHAEILTDGRLRIGIAQKAVN
                     LYLKYLWCADWITPPPPHCPIDGIILDMAGIHDVRWTTDIVTIEDYQNVIKRIREKIG
                     DKPLADWELGAYNSNP"
     gene            complement(3233..>3843)
                     /locus_tag="PKH10_08460"
     CDS             complement(3233..>3843)
                     /locus_tag="PKH10_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28196.1"
                     /translation="KFISLIEDHLLFAPELKLENQISTIISVFLNRKGIRAEREVSDR
                     DIKRCDLVIEEKTIEAKYHFEGDLIEVKNEVIGRIDQIRTGDFDKEEKKRPGAGIEVV
                     HDMMRKDRDYFLWYLCVRKNESSEEGRYRYKGKKYRYKYPKGTEIFYQTTSCQYEQSS
                     KVVLENIREMNECLETIGIISYQLPPIETEKATLWTFLYKLK"
BASE COUNT          838 a         1019 c         1063 g          923 t
ORIGIN      
        1 caccgggatg ctcgacgacg ccttttcgcg ccgtttcctc tacaagttgg aattcccgcg
       61 cccggccccg gccatccgcg ccctgatctg gcgctcgaaa ctgcccgaac tgtcggagga
      121 ggaggccgat gcgctctcgg cgttcgacct gtccggcggg gagatagaga atgtgacgcg
      181 ccgctacatc gtcgatacgc tcttcagcgg caagcccgac ctcgagcggt tggaggtgct
      241 ttgtgaacag gagtcgtcgc tgaagaaagg gaccggaaga aaggtggggt tttgacttct
      301 cctcgccctg tggagggaga ggatcgaggt gagggtgttc tgaaaccctc atccctgcct
      361 tctccctcga taggtagaag gatgttttag ggaggtgatc atggttcagc agttcaggcc
      421 gtatgtgggg agggtgctct ctggttctct cacttccacg gaccgggcgc gatccagcgg
      481 tcggtgaacg aggtcccttt cttgtgtgcc tcgacgagcg gcccgaggaa cagggcgacc
      541 gcccgtacga tcagcgcgaa ggaggcggga acatcactcg gcgacatcct ctttatgaaa
      601 gcactccact gaacttgatg ggtatccgcg aactggtcgg agaatgccac gatcggatcg
      661 ggtacggata tccccctctt cgacagtgtc cggtctatcg cctcaagtag aacggcgccg
      721 tcaaaatcaa atcgacggga gagccaccag atatcgtgaa aatctttcat ccggctgttc
      781 aactggccgt gggtcagcat ggcatggagc ttttcggcga tggcgctttc acggctgtag
      841 cacgagataa cgggggccgg aagatcgaga atggtgggga aattctcctt ttgaggaggt
      901 ggatagatcg ggtcaccgaa cccgatgtcg atctggaggt ggagccgcat cgtatcgaga
      961 agcgccggta tctgaacacg aagtccccgg tactccgccc cttcggttat ccgttcgatc
     1021 accatatcga cggcagagaa ctctaggccg tccggctcta cctcgctcgc cagcactttc
     1081 cgtatcgctg cggcggtcgc ggcctcttcg ttcgataccc ggccgagaag atcgatatcc
     1141 atcgtcgccc gcgcctctgg cgcctgccag actcgtagca taagcgcccc cttcaggaca
     1201 aaattctctt tctgcgatga gcgcgacaag cgatagagaa atcgttccat gaggtaactt
     1261 tgcgcgatct cctgaaagga acgattccct tcgcgagcct tgttgaggag ccgttgccgt
     1321 accgaagccg ccagattcgt tatcggcttc ataggagcgc ctccagatac ggtttcatga
     1381 gccgttccac gcgacaggtg cggccgtatg ccagaagcgc atcgacatcg aacctccggc
     1441 gtgcccggta gagtttcagc gcctcggcaa agatatcgaa acctatcttg ttgcggaact
     1501 tgaggcagtc ggcgatcgtt ttctccgggc tgaatatgcg cacggcgatc ccgtcgaaca
     1561 gatgtttctc cacccccgcc gaaagacttt cttccgaaaa acggaaaacc gacagcgggg
     1621 ggaaatcgat gcgcgggctc cgcgccgatg aagcgatggc aatggaaacc gtggcgggcc
     1681 tttgcgtggt caggttgtga aaagagagcg ccgaaacaag acaaataacg gcatgcggca
     1741 cccgtgcggc gacggtcgca aggtccggtt gcgtaagttc cggcaggtcg gcgagccgga
     1801 atatgccccg ggacacggtc gtcagttgtc cggtgtcgcg caggagatag agcgttcgtg
     1861 ggtggatccc ctttttgatc gcctcagcgg tgtgtatgat accgccggtc cgttcaatga
     1921 tcttcagtgc tttctggtcg gccggcatga tccctcccca cttgcgtgga aattatagtc
     1981 tgtagttaca aacaaccaca gacgctatta tccgcacggt catgtaaaac acaagaaaaa
     2041 taatgccccc gcagggactc ccggtgtccc tgtagttgtg ttattctgtt actgtcgggg
     2101 ccgggtggtc ccggccgtgc cgatttacac cgacttgcgg gcacgctaaa cgaaccgcta
     2161 aaaggaggcc atcatgtcgc aacagttcac gccgtatgtg acccggcggc tcatcgccgc
     2221 aatttaccag cgcatcgccg tcccgcccga atgtgtgacc gaggaggaga tgatcacctt
     2281 tacgcgggcg acgacccaag ggcatttcaa atgcgttctg gtgcccggcc ccggccgcgc
     2341 gatctggttc aatgaaaagg gagagatcac cgggcgcgac gaaacgggag cgaagttgcc
     2401 agaggcgcgg cccgatgtgg ggagagtggt gttttagtga ctacaagaaa tgtctaatgt
     2461 cgaggaccga ccccgcttac ttccgtactt ccgagatcct caagaagatg gagaaggggc
     2521 tgttgaccgt cgagttcacc gcgaaatatc tggcccggtt cctcaagagt ggcacgctga
     2581 gcagacagga cttccttgag ttcacgcagg tggatgagat ccggacgagg tacaaaacga
     2641 tagaaggaga ggtagggaag atttgagtta aggcgggcat tcgactacgg gttactgttg
     2701 tatgccccaa gttcccaatc ggcaaggggt ttgtcaccga ttttctctct gatcctttta
     2761 attacatttt ggtaatcttc aatggtcacg atgtcggtgg tccatcgcac atcatgaatc
     2821 cccgccatat cgagaatgat tccgtcgatg ggacagtgag gcggcggtgg cgtgatccag
     2881 tcagcgcacc agagatattt caggtagagg ttcaccgctt tctgcgcgat gccgatacga
     2941 agtcggccgt cagtcagtat ttccgcatgt tttgacgaga gttcttcggc tatccgctga
     3001 atgttccggc agtgttcctc gccatccacg accatgcggt agggttcttc cagcatccgg
     3061 agtttagcgt ggaggtcttt gataaaatca tcgcgccgct ttgtaaactc cttggtatcg
     3121 attccctccc ggtagacatg ggcgcgctga aaagcgcctt gttgggcgtt ggaaaggaac
     3181 acggaacgga ggaattcgag ttgtttgtca tccatagaaa gaatctctct cgtcacttca
     3241 atttataaag gaaggtccag agcgtcgcct tctctgtttc tataggcggc aactgataag
     3301 agatgattcc gatggtctcc aaacactcgt tcatttctct gatattctca agtacgacct
     3361 ttgaggattg ttcatattgg caagaggttg tctgataaaa gatttcagtt cccttcggat
     3421 acttatagcg atatttcttc cctttgtatc tatatctgcc ttcttctgaa gattcgttct
     3481 tcctgacgca aagataccac aaaaaataat cgcggtcctt tctcatcatg tcatgaacca
     3541 cctctatacc ggcaccgggt ctttttttct cttctttatc aaagtctcct gttcttattt
     3601 gatcgattct tcctattacc tcattcttta cctcaataag gtcgccctca aaatgatact
     3661 ttgcttctat tgtcttttct tcaatgacca aatcgcatct ttttatatcg cgatctgaaa
     3721 cttctctttc tgcccgaatg ccttttctat tgagaaagac agaaataatc gtggatatct
     3781 gattttccag tttgagttcg ggcgcgaaaa gaagatggtc ctcaatcaga gagataaact
     3841 tct
//
LOCUS       DAOQUE010000262         3808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_27135_length_3808_cov_3.771649, whole genome shotgun sequence.
ACCESSION   DAOQUE010000262 DAOQUE010000000
VERSION     DAOQUE010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27135_length_3808_cov_3.771649"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PKH10_08465"
     CDS             <1..852
                     /locus_tag="PKH10_08465"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT28197.1"
                     /translation="LRRAHGLLGLHPELNDRVADLCLRDDLHSDARKLILTVLTSHDL
                     PETQAAMRKILNGDTVKHDPAYPMLLQAAGFLATPDKETAALVQGVLREEHGEKRYAA
                     AYTLGVIVGTMRESGRDNEARTHNRELVARLEQAESAEEQQRLLVALANAGMPENVPV
                     ARRYARDGNPETRTAAAMALRKTQTEDSERALFDLTGDGDRNVQNTALLMLSKYRVGK
                     NHLDEIGAKIGQGAITEESYQMVLNLLAPLRGTQGDAVRDLCRRMLEKGVKDANLAQQ
                     IRELMER"
     gene            922..1917
                     /locus_tag="PKH10_08470"
     CDS             922..1917
                     /locus_tag="PKH10_08470"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT28198.1"
                     /translation="MPKHLLFLAVIFLLVACEDSVKRGPRLWISYGGLEWSKQREGWL
                     NWYEAKEYCASIGERLPTVDEIKTLFPYCKKIQKNGSCQLSDPECLTFDCWETGCSCD
                     ERKGDRVPSMLGDDSVDLWLSSVFMSDDRQAFVFRVWWDFGEFGMEYKERQRAELRCV
                     KLTPEEINDTEKPDDITEYGLFWSERGPFMTWQEADDYCEALEEDGHDDWRLPTINEL
                     RTIIINCPDTMPGGSCNVSDPECLDETCSKDCSCEYENTPHDNFSYSSLGDSEIVRLW
                     SSSITPPYTPQPSYHLVWKVAFSDDSIYSSLPDNGGDVRCVRTPSRSEQIRELME"
     gene            1962..2927
                     /locus_tag="PKH10_08475"
     CDS             1962..2927
                     /locus_tag="PKH10_08475"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNT28199.1"
                     /translation="MNGPLRHIPDLTPRAAFPEAADDAFIRMTQALRAADGDPSKLPE
                     PLSIRYQHNLAANLMKEKVFFMVSEKLRAGGIAEVYPIKGMHLLLGLYRDLPGIRTMV
                     DIDLMVRKSDFTKLPGIVRANPAWQPDMARLLDLRAHIATDISFIADHTSVEVKRDLI
                     LMPVVDPAPFFDAAKTVTVAGKEARLLQPDHAALLYILHHIGDHLLHYRRIEHRHLAE
                     FSVILADLGDTPAFRELCRAAGLERWYDLILFLIYTLFERPVVTPADFTIHPIFSCIV
                     KRRDGVLGLTAAARLTAFLYGAWAVPLFTRNALLWLPKKMVRRGH"
     gene            complement(3374..>3808)
                     /locus_tag="PKH10_08480"
     CDS             complement(3374..>3808)
                     /locus_tag="PKH10_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28200.1"
                     /translation="YIEVVVGVADGYLYAIDQKRYTETFPTREVEVKSGKITKPGTDI
                     DSQSYAGQIGVAWSPVFKAKGYDVRIVDTAGTVISDTVEVLNGFQTIITDADIVNGQT
                     YYAEVRGYDDEGYYSDWSRGDGVTVNVATATSLLSRIRSLFR"
BASE COUNT          797 a         1191 c         1086 g          734 t
ORIGIN      
        1 ctccggcgcg cccacggcct gctcggcctc caccccgaac tgaacgaccg ggtcgccgac
       61 ctctgcctgc gcgacgacct gcacagcgac gcccgcaaac tgatactgac ggtactcacc
      121 agccacgacc tgcccgagac acaggcggcg atgcgcaaga tactgaacgg cgatacggtg
      181 aagcacgacc ccgcctaccc gatgcttctg caggcggccg ggttcctcgc cacgccggac
      241 aaagagacgg ccgcgctggt gcagggtgtc ctgcgcgagg agcacggcga aaaacggtat
      301 gccgccgcct acacgctcgg tgtgatcgtc ggcaccatgc gggaaagcgg acgcgacaac
      361 gaagcgcgga cacataaccg ggaactggtc gcgcgactgg agcaggcgga gagcgccgag
      421 gagcaacagc gcctgcttgt cgcactcgcc aacgccggca tgccggagaa tgtcccggtg
      481 gcgcgtcgct acgcccgcga cgggaacccc gaaacacgga cggcggcggc gatggcgctc
      541 cgcaagacgc agaccgagga ttcggaacgg gcgctattcg acctcaccgg cgacggcgac
      601 cggaatgtgc agaataccgc tttgctgatg ctttcgaaat accgcgtggg caaaaaccac
      661 ctcgacgaga tcggggcgaa gatcgggcag ggcgccatca ccgaggaaag ttaccagatg
      721 gtgctgaacc tgctcgcgcc gctccgcggg acgcagggcg acgcggtgcg cgacctctgc
      781 cggcggatgc tcgaaaaagg ggtcaaggac gcgaacctcg cccagcagat acgggaattg
      841 atggagcgtt gaagtactat gcgatacggc gcggtaacga cgatcatcga tgttgcgccc
      901 accaagagaa agagggtgat catgccgaaa catctcctgt tccttgcggt gatattcctg
      961 ctcgtcgcct gcgaggattc tgtcaagagg ggaccgaggc tttggatctc ttacggcgga
     1021 cttgaatggt cgaaacaaag ggaaggatgg cttaactggt acgaggcaaa agagtactgc
     1081 gcctcgatcg gagagcgcct cccaacggtc gatgagataa aaacgctctt cccgtactgt
     1141 aagaagatac agaagaacgg ctcctgtcaa ttatctgacc cggaatgtct gacctttgat
     1201 tgttgggaaa ccggatgctc atgtgatgaa cgcaaaggag atcgggtacc atccatgctt
     1261 ggagacgaca gtgtcgactt gtggttatcc tctgtcttta tgagcgatga ccggcaggct
     1321 tttgtttttc gtgtatggtg ggatttcgga gaatttggca tggaatataa agaacgccag
     1381 agagcagagc tgcgttgtgt gaaactcacc cccgaagaga taaacgacac cgaaaaaccg
     1441 gatgacataa ccgaatacgg tctcttttgg tcagaaaggg ggcctttcat gacttggcag
     1501 gaggcggatg actactgcga agcccttgaa gaggacgggc atgacgattg gcgactgccc
     1561 accatcaacg aactacgcac cataatcatt aactgtcctg ataccatgcc gggcgggtcc
     1621 tgcaatgttt cagatccaga gtgcttggat gaaacttgta gcaaagactg ctcatgtgaa
     1681 tatgagaata cccctcatga caacttctct tattccagcc taggggatag cgaaatcgtc
     1741 aggctttggt cttcttctat tactcctccg tacaccccgc aaccatctta ccacctagtg
     1801 tggaaggttg ctttttccga tgattctata tattccagcc tcccagacaa tgggggcgat
     1861 gtccgctgcg tgagaacgcc gtcccgctcc gaacagatac gggaactgat ggaataaccc
     1921 ccttcctttg acttcttggt ccgcgccggt agaatggcgg catgaacgga cccctgcgcc
     1981 acatccccga cctgacgccg cgcgccgcct tccccgaggc ggccgacgac gccttcatcc
     2041 gcatgacgca ggccctgcgg gcggccgacg gcgacccgtc gaaattgccc gagccgctct
     2101 cgatacgcta tcagcacaat ctcgccgcga acctgatgaa ggaaaaggtc ttcttcatgg
     2161 tgtcggagaa actgcgcgcc ggcggcatcg cggaggtcta cccgataaag gggatgcacc
     2221 tgctcctcgg gctgtaccgc gacctaccgg gcatccggac gatggtcgat atagacctca
     2281 tggtgcgcaa aagcgatttc acgaaactgc ccggcatcgt gcgggccaac ccggcgtggc
     2341 aacccgacat ggcgcgcctc ctcgacctgc gggcgcatat cgccaccgac atatcgttca
     2401 tcgccgatca tacctcggtc gaggtgaagc gcgatctcat cctgatgccg gtcgtcgacc
     2461 ctgccccctt cttcgacgcc gccaagaccg tcaccgtcgc cgggaaagag gcgcgactgc
     2521 tccagcccga ccacgcggca ctgctgtata tcctgcacca catcggcgat cacctgctcc
     2581 actaccggcg catcgagcac cgccacctcg ccgagttcag cgtcatcctc gccgatctcg
     2641 gcgacacccc ggcgttccgc gaactgtgtc gtgcggcagg cctcgaacgg tggtacgacc
     2701 tcatcctgtt cctcatctac acgctgttcg aacgcccggt cgtcaccccc gccgacttca
     2761 ccatccatcc gatattctcc tgcatcgtga aacggcgcga cggcgtgctc ggcctcacgg
     2821 ccgccgcgcg gctcaccgcc ttcctctacg gcgcgtgggc cgtgccgctg ttcacccgca
     2881 acgcgctttt gtggctgccg aagaagatgg tgcggcgggg acattaaacc ccgtcaaggc
     2941 cttgtctttg ttaaaccctt ccctttaatt cccttccctt gacagggcag ggggcggtat
     3001 ttttcccatt caaacgcgag cacctgtctg agcggagttt atcgaagcga gttgcgcagc
     3061 gtcccgtcag accctcttct ttgcttcctt tcttcttggt ctgaagaaga aaggaagacg
     3121 cctacagcgt gcgtcagcag gcgaggcgga atgatcgcat cgcgctcgcc gaaacgctcc
     3181 ccttcccaga ccctcatccc tgccttctcc ctctattagg tagaaggtga taaaaaaagg
     3241 acggggcgcc ctgccccggc ttcgcttacg ctatgccgtg acaaggcgcc cctatggggt
     3301 gggagggggt tttgatccaa ctcacccctg tcccctctct tgccaagaga ggggtgccgc
     3361 gaggcggggt gagttatcta aacaaactcc tgatcctcga caagagcgag gtggccgtcg
     3421 ccacattcac cgtgacgccg tcaccgcgac tccagtcgct gtagtatcct tcgtcatcat
     3481 agccacgcac ctcggcgtag taggtctgcc cgttcacgat atcggcgtcg gtgatgatag
     3541 tctggaagcc gttcagtacc tcgaccgtat cggatatgac cgtgcccgcc gtatcgacga
     3601 tccgcacatc gtagcccttc gccttgaaga ccggactcca cgcgacgccg atctgaccgg
     3661 catacgactg cgagtcgata tcggtccccg gcttggttat cttgccgctc ttcacctcaa
     3721 cctcgcgggt cgggaaggtc tcggtgtagc gcttctgatc gatcgcgtag agatagccgt
     3781 cggcgacgcc gaccaccacc tcgatata
//
LOCUS       DAOQUE010000263         3789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_27327_length_3789_cov_4.570702, whole genome shotgun sequence.
ACCESSION   DAOQUE010000263 DAOQUE010000000
VERSION     DAOQUE010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27327_length_3789_cov_4.570702"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..78)
                     /locus_tag="PKH10_08485"
                     /pseudo
     CDS             complement(1..78)
                     /locus_tag="PKH10_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-nitroimidazole antibiotic resistance protein"
     gene            223..1467
                     /locus_tag="PKH10_08490"
     CDS             223..1467
                     /locus_tag="PKH10_08490"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNT28201.1"
                     /translation="MSLTDLAKVAADRGATAPFDGSETGLRAAIDALVPQIAATGFSE
                     KTLAEERDWRAAGQELVIVEFTSAAPGATVTVDGKLLCSALPCRKNIVKGKHAILLQA
                     PHYLALAEDITVDAPATVTRDLKPDFALLSVTTIPAGLAVEIDRRPAGVSPVKMRMPP
                     GLHRVMINSPCHLQKGLELALQRGEERTVQIAPAPRMAGISVISEDKAGNAVAAPVLV
                     DGEKVGTAPGSFTVPLCSKQLVVQISDDDKFETDLNLRENHTDEFRATLKTELDAIAA
                     DGHAKAKAFDDKKELPPVRPYLEPGAWVLASGIVVTGIAAGLAVAAMEKFDDYQEFTS
                     PATVAQEMVKPSFDVAAYQSRADKIYGDAEALEYTGIALFAAGGAAVATGIILMCITG
                     EAPPVTATVTPEGVYLGFGGRF"
     gene            1467..>3789
                     /locus_tag="PKH10_08495"
     CDS             1467..>3789
                     /locus_tag="PKH10_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT28202.1"
                     /translation="MGNDMKKTPNRRGAPTARPIHATDPRRGAIHCAQYQRTTGSGVM
                     NHAPTNTGRAVGAPLQVGLLFLCALFFFGCDHPEKVDIGELVEALNALDADASVDADG
                     TEIDIIDKGTSDDEDCCVTDPGDFEYPVVDDAVVADEDVVEAEDDLLTETEVEDDVVP
                     ESESVVEDDLVIIDDSVVPDEDTVETFFIDGLVAWWKLDGNGEDFIGPYDTFTGTGYT
                     FYSETRSGAGQSANGSAVYDVSMDDIVTANAGFTISTWVYYTKSDSYLNGFLTWTSGV
                     SSSDEGSSVYFDGNGSRVYENGVQQIDYSHNRNAGQWYHYVWTYDAGAFELYIDNELR
                     DLGSVPFTTHYVAKFVIGQTYGSPDHTSDEARIDDVMLFDRALSSQELLGLFELGRNG
                     YQGSELVHKEVLPSSVCSGQIKCYNNTSETLCPPEGNDFYGQDGMYAAMGYCVPRSYT
                     VSGVVPEEIVIDNNTGLEWQRTLPATYDGCTGGTCKWQEAIDYCSNLTYGGETDWRLP
                     TRKELATLPDYGRENPAIDTAIFPDTQPGGYWSSSSNVNGTTDAWLVDFNVGSVSHSG
                     KTNDNYARCVRGSALPVSSFTETTMASKVIVTDTEMRLIWTKEYSGQITWQNALSYCE
                     NLSYGGATDWRLPNIDELKTLIDDTIYAPASTFPEMPSSFSFYFWSSSSYVPYTTYAW
                     AVPFYEGNVQLYDKSGTIYARCVRDATLTRTTKQWGTSQTEYGRSVAIDSNGNIFVTG
                     HTGGDLDGNTNAGNGYDIFLTKWNANGTKAWTKE"
BASE COUNT          844 a         1079 c         1118 g          748 t
ORIGIN      
        1 aaagtacagc ctgccctccg cataaacata gctcaacggg acagcatagg ggtaaccgtc
       61 atcgcccgcg accgccagaa ggaggcggcg ctctccctgc gcaaggagag ccatcaggaa
      121 tgttacgaca agacctgcaa catacaactg gcccagcggc tcgccgccga caccatgctc
      181 gagacccgcg tcaccttctt cggcggggaa tacacgctct cgatgagtct caccgatctc
      241 gccaaggtcg cggccgaccg cggcgccacc gcgccgttcg acggctccga gaccggcctg
      301 cgcgccgcga tagacgcact cgtcccgcag atagcggcga ccggcttttc cgaaaagacg
      361 ctcgccgagg agcgcgactg gcgcgccgcc gggcaggaac tggtcatcgt cgagttcacg
      421 agcgccgccc cgggcgccac cgtcacggtg gacggcaaac tgctgtgtag cgcgctcccg
      481 tgccgcaaga acatcgtgaa ggggaaacac gcgatactcc tgcaggcccc ccactacctc
      541 gccctcgccg aggacatcac cgtcgacgcg cccgccaccg tcacgcgcga cctcaagccc
      601 gacttcgccc tgctctcggt caccaccatc cccgccgggc tcgcggtcga gatagatcgc
      661 cgccccgccg gggtgtcgcc ggtcaagatg cggatgcccc ccgggctcca tcgcgtcatg
      721 ataaattccc cgtgccacct gcagaaaggg ctcgaactcg cgctccagcg cggcgaggag
      781 cggaccgttc agatagcgcc cgccccgcgc atggcgggga tatcggtcat atccgaggat
      841 aaggccggta acgcggtcgc cgctcccgtc ctcgtggacg gcgagaaggt cggcaccgcc
      901 cccggcagtt tcaccgtgcc gctctgctcg aagcaactcg tcgtccagat aagcgacgac
      961 gacaagttcg agaccgacct caacctccgc gagaaccata ccgacgaatt ccgcgccacg
     1021 ctcaagaccg aactcgacgc catcgccgcc gacggccacg ccaaggcgaa ggcgttcgac
     1081 gacaagaagg agttaccgcc cgtccgcccc tatctcgaac cgggtgcgtg ggtgctcgcg
     1141 tcgggcatcg tggtgaccgg catcgcggcg gggctcgcgg tcgcggccat ggagaaattc
     1201 gacgactatc aggaattcac ctcgcccgcg acggtcgcgc aggagatggt gaagccctcc
     1261 ttcgatgtcg cggcgtacca gagccgcgcc gataagatat acggcgacgc cgaggcgctc
     1321 gaatacaccg gcatcgcgct ctttgccgca ggcggcgcgg cggtggcgac cggcatcata
     1381 ctcatgtgca tcaccgggga ggcgccgccg gtcaccgcga cggtgacgcc ggagggtgta
     1441 tatctcgggt ttggagggag gttctgatgg ggaatgatat gaagaaaaca ccgaatcgta
     1501 ggggcgcgcc aacggcacgc ccgattcatg caaccgatcc ccgtaggggc gcaattcatt
     1561 gcgcccaata tcaaagaacg accggctcgg gcgtgatgaa tcacgcccct acaaataccg
     1621 ggcgtgccgt tggcgcgccc ctacaggtcg ggttgttatt cctctgcgcc ctgttttttt
     1681 tcgggtgcga tcatccggag aaggtggata tcggggaatt ggtcgaggcg ctcaatgcgc
     1741 tcgatgcgga tgcctctgtt gatgccgacg ggacggagat agacatcatc gacaagggga
     1801 cttcggacga tgaggattgc tgcgtcacgg atccgggcga ttttgaatac ccggtagtgg
     1861 atgacgcggt ggtggcggat gaggatgtgg tggaggcgga ggacgatctg ctcacggaaa
     1921 ccgaagttga ggatgatgtg gtgccggagt cggagagcgt cgttgaagat gatctggtca
     1981 taattgacga tagtgtggtg ccggatgagg atacggtcga gacctttttc atagatggcc
     2041 ttgtggcatg gtggaaactg gacggcaatg gcgaggattt catcggccct tacgacacct
     2101 ttacgggaac cggctacacc ttttacagcg aaacccgcag cggcgccggc cagtcggcga
     2161 atggatcagc cgtttatgat gtgtcgatgg acgacatagt taccgccaat gccgggttca
     2221 ccatatcgac ttgggtctat tacaccaaat cggattctta cctgaacggg tttcttactt
     2281 ggacatccgg ggtatctagt agcgatgagg gatcttctgt ttatttcgat ggcaacggta
     2341 gtcgtgtcta tgaaaacggc gtccagcaaa ttgactattc tcacaatcgg aatgccggtc
     2401 agtggtacca ttatgtttgg acttacgatg cgggcgcatt tgaattatac atagacaatg
     2461 aattaaggga tttgggttct gttccattta ccacgcatta cgtagcgaaa ttcgttattg
     2521 gtcagaccta cggttctcct gatcatacaa gtgatgaagc aaggatagac gatgtgatgc
     2581 tgttcgaccg ggctttgtca tcgcaagaat tactggggct gtttgaatta gggcgaaatg
     2641 gttatcaagg cagtgagctt gtgcataaag aggtgctccc ctcttctgtt tgctcagggc
     2701 aaatcaaatg ctacaacaac acatctgaaa ccttgtgtcc gcccgagggg aacgatttct
     2761 atgggcaaga tgggatgtat gcggctatgg ggtattgtgt gccgcgaagt tacaccgtca
     2821 gtggtgtcgt gcccgaagag atagttattg acaacaacac cgggcttgaa tggcagagaa
     2881 cgcttccggc gacttatgac gggtgcaccg gtgggacatg caaatggcag gaggcaatag
     2941 attattgtag caacctcact tacggtgggg aaaccgactg gcggttgccg acccgcaaag
     3001 aacttgcgac ccttcccgac tatggccgcg aaaatccagc gatcgatacc gccatctttc
     3061 ccgacacaca accaggtggt tactggtctt cctcgtccaa tgtgaacggt accaccgatg
     3121 catggcttgt ggatttcaat gtcggctcag tgagccatag cggtaagacc aatgacaact
     3181 atgcccgttg tgtgaggggt tcggcattgc cggtgagttc gttcaccgaa acgacgatgg
     3241 ccagcaaggt aatagtcacc gacactgaaa tgaggcttat atggacaaag gagtacagcg
     3301 gccaaatcac atggcagaat gccttgagtt attgtgaaaa cctttcctat ggcggcgcca
     3361 ccgattggcg gttgccaaac atagatgaac tgaaaacact gatagacgac accatttacg
     3421 ctcccgcgag cacttttccg gagatgccat cctctttctc cttctacttc tggtcttcct
     3481 catcctatgt gccctatacc acctacgcat gggcggtgcc tttctacgag ggcaatgtgc
     3541 aactttacga taagagcggt acaatctatg cccggtgcgt acgagatgcc accttaacac
     3601 gcacgaccaa acagtgggga acctctcaaa cagagtacgg tcgttcggtc gcgattgaca
     3661 gcaatggaaa cattttcgtc accgggcaca ccggcggcga ccttgacgga aacacgaacg
     3721 cggggaacgg ctacgatatc tttctgacca aatggaacgc caacggcacg aaggcgtgga
     3781 ccaaagagt
//
LOCUS       DAOQUE010000264         3766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_27555_length_3766_cov_4.120183, whole genome shotgun sequence.
ACCESSION   DAOQUE010000264 DAOQUE010000000
VERSION     DAOQUE010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27555_length_3766_cov_4.120183"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1028)
                     /locus_tag="PKH10_08500"
     CDS             complement(252..1028)
                     /locus_tag="PKH10_08500"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNT28203.1"
                     /translation="MEKELRVGPLSFTSRLFLGTGKYRSNDAMIAAVKAAGTQVVTAS
                     LRRMDLGGKDESLLKPLLNCPDITILPNTAGARTADEAVFYAEMAAEAGLPKWVKLEL
                     TPEPRYLLPDPIETLKATEILVKKGFAVLPYINADPMLALRLQDAGAATVMPLAAPIG
                     TNKGLLMREMIRIIIEQARVPVVVDAGIGAPSDAAAAIELGADAVLVNTAIATAKDPA
                     AMGTAFKMAVEAALVARDAGIPKKYDSAQASSPELDLLKK"
     gene            complement(1033..1944)
                     /gene="nadA"
                     /locus_tag="PKH10_08505"
     CDS             complement(1033..1944)
                     /gene="nadA"
                     /locus_tag="PKH10_08505"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNT28204.1"
                     /translation="MDDLIRRIAALKKEKNAIVLAHTYTRAEVQDIADVVGDSFELAV
                     RAAGSSAEIIVLAGVRFMGETAAILTPGRTVLLPVADADCPLADMITSDQVRHLKAQY
                     PGAAVACYVNSSVEVKALSDVCVTSSSAVRIVRNLPQRQVLFVPDQNLGSWVAEQVPE
                     KEIILHSGFCPVHHQIKPDDVRDARKLFPGAVVLMHPECTKETRAEADHILSTGGMVA
                     LVKEKKHTRYIIVTESGITHTLRRHDPAAEFIELSAPMLYCQNMKKITLETILRALDE
                     GETVVTVASDIAERARYALSRMLELNR"
     gene            complement(1944..2189)
                     /locus_tag="PKH10_08510"
     CDS             complement(1944..2189)
                     /locus_tag="PKH10_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNT28205.1"
                     /translation="MTESAKNDWKKKVPEVLEKVRPMLQRDGGDVELIGVGDDGVVQV
                     RLKGACHGCPMSQMTLKMGVEFELKRNIPEVTKVVSI"
     gene            complement(2285..3655)
                     /gene="dacB"
                     /locus_tag="PKH10_08515"
     CDS             complement(2285..3655)
                     /gene="dacB"
                     /locus_tag="PKH10_08515"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNT28206.1"
                     /translation="MRTLVFLPAIILPLLLGAADTTQQPFAPDLGIIAPEDAGIVLYD
                     ITAKKEAFSLNRDKEFPYASNVKLITTGAALKHLGRNYTFKTRFYYKPETATLYMKTQ
                     GDPTMVMEELWLVANELKNRGVKYIARVVADDYAYGEKGSIPMAGNDGTGRAYNAYLS
                     PFALNYNSVELVFLPGKEGEKAQVFVSTPGRHFILEGEVMTVKGKEKKVEILSENADG
                     RTKLTVSGTIGTELKEGLRKERKIWDPLRHYVDTLMYFMFQAKDVVPDRARLDDSLFD
                     AADGIRYTHESRELFLILTFMNRWSTNFIADAIMYAIGLEKKKDHRAGLAMLKQYAKE
                     LTGVEPGIINACGLGTVAQNLLTPGFVMELMKKEYASPFSMPDLFATLPVAGEEGTMK
                     KIKADYKHEARFKTGSLSFVAAVSGLMRAKSGKVYLVTLVLNSKKKAYLVPERDLILN
                     QIWQAF"
BASE COUNT          647 a         1072 c         1218 g          829 t
ORIGIN      
        1 ttcttgcgat gaaattcgtc gagcgccgcg tcgagttcgg gcaggtcgtt ggtgaagcgg
       61 gaaaggagca cctcgtcatg gtattcctgc ataagccgca gaagtcgcgg gcggcgcttg
      121 ttctgccgtg ccttcgggtt ggatatgagg ccgatgcctg ccatggctcc tctggtaagg
      181 gtaaagcgtc atatattagg tatagttctg tgcgattgtc aactaaataa gggccgaaat
      241 aagggccgat gtcacttttt caggagatcg agttcggggc tggaagcctg cgcgctgtcg
      301 tacttcttcg ggatgccggc gtcgcgggcg acgagcgccg cttcgaccgc catcttgaat
      361 gccgtgccca tcgcggcggg gtctttggcg gtggcgatgg ccgtattcac gagcaccgcg
      421 tcggcgccga gttctatcgc cgcggcggcg tccgagggcg cgccgatacc ggcgtcgacc
      481 acgaccggaa cgcgcgcctg ttcgatgatg atgcggatca tctcacgcat gaggagtcct
      541 ttgttcgtcc ctatcggcgc ggcgagcggc atcaccgtcg cggcgcccgc atcctgtaac
      601 cgaagcgcca gcatcgggtc ggcgttgatg taggggagga ccgcgaagcc cttcttcacc
      661 agtatctcgg tcgccttcaa ggtctctatc ggatcgggca ggagatagcg cggttcgggg
      721 gtgagttcga gtttcaccca cttgggaaga cccgcctcgg ccgccatctc ggcgtagaag
      781 accgcctcgt cggccgtccg cgccccggcg gtgttgggca gtatcgtgat gtcgggacag
      841 ttaaggagcg gctttaagag cgattcgtcc ttgccgccga gatccatccg ccgcagcgat
      901 gccgtcacca cctgtgtgcc ggcggccttc accgccgcga tcatcgcgtc gttcgatcgg
      961 tatttgccgg taccaaggaa gagccgcgag gtgaaagaga gcgggccgac gcgcagttct
     1021 ttttccatta tctcacctgt tcagttcaag catccggctg agggcgtaac gcgcccgctc
     1081 ggcgatgtcg cttgccaccg tgaccaccgt ctctccctcg tcgagcgccc ggaggatggt
     1141 ctcaagcgtt atcttcttca tgttctggca ataaagcatc ggggcggata gttcgatgaa
     1201 ttcggccgcc gggtcgtgtc gccggagggt gtgggtgatg cccgattcgg tgacgatgat
     1261 gtagcgcgta tgtttctttt ccttcacgag cgccaccatc ccgccggtgg aaagtatgtg
     1321 atcggcctcg gcgcgcgttt ccttggtgca ttcgggatgc atgaggacca ccgcgccggg
     1381 gaagagtttc cgcgcgtcgc ggacatcgtc gggttttatc tggtgatgga ccgggcaaaa
     1441 gccgctgtgg aggatgatct ccttttcggg cacctgctcg gcgacccatg aaccgaggtt
     1501 ttggtcggga acgaatagca cctgtctctg cggcaggttg cggacgatgc ggaccgccga
     1561 cgaactggta acgcagacat cggagagcgc tttgacctcg accgaggaat tgacatagca
     1621 ggcgaccgcc gcgccgggat actgcgcctt gaggtgtcgc acctggtcgg acgttatcat
     1681 gtcagcgagc ggacaatcgg catcggcgac cggcaggagc accgtgcggc cgggggtgag
     1741 gatggcggcc gtctcgccca tgaaccggac gccggcgagc acgatgatct cggcggagga
     1801 gccggcggcg cggaccgcca gttcgaacga gtcaccgacc acatcggcga tatcctgcac
     1861 ctcggcccgg gtgtaggtgt gggcgagaac aatggcgttc ttttcctttt tcagcgccgc
     1921 gatgcggcgg ataaggtcat ccatcagatg ctgacgacct tagttacttc gggtatgttc
     1981 cgcttgagtt cgaactcgac gcccatcttg agcgtcatct gtgacatcgg acagccatgg
     2041 cacgcaccct tgagccgcac ctgcaccacc ccgtcgtcgc cgacgccgat cagttcgaca
     2101 tcaccgccgt cgcgttgcaa catcgggcgt actttttcga gtacctcggg aaccttcttt
     2161 ttccagtcgt tcttggcgct ctcggtcatg ggactctcct tcgttcaccc gacggtcttc
     2221 gtcgcgcttc cgcatacgat aacgccggaa ggtggtaagt caagggcatg cagggaaaag
     2281 tgggtcagaa cgcctgccat atctggttaa ggatgaggtc acgctcgggg acgaggtagg
     2341 ccttcttctt cgaattgagt acgagcgtga cgagatagac cttgccgctc ttcgcgcgca
     2401 tgagtcccga caccgccgcg acgaacgaga gcgagccggt cttgaaccgc gcttcgtgtt
     2461 tgtagtcggc ctttatcttc ttcatcgtcc cttcctctcc cgccaccggg agcgtcgcga
     2521 agaggtcggg cattgagaag ggtgaggcgt attctttttt catcagttcc atgacgaagc
     2581 cgggcgtcag caggttctgc gcgacggtgc cgagcccgca ggcgttgatg atgcccggtt
     2641 cgacaccggt cagttccttc gcgtactgtt tgagcatggc gagtccggcg cggtggtcct
     2701 tctttttctc aagaccgatg gcgtacatga tcgcatcggc gatgaagttg gtggaccagc
     2761 ggttcatgaa ggtgaggatg aggaagagtt ccctgctctc gtgggtatag cggatgccgt
     2821 cggcggcgtc gaagagcgag tcgtcgagtc gcgcccgatc ggggacgaca tccttcgctt
     2881 ggaacatgaa gtacatgagc gtgtcgacat agtgccggag cgggtcccat atcttccgtt
     2941 ccttgcggag gccttccttg agttcggtgc cgatggtgcc cgacacggtg agttttgtcc
     3001 gtccgtcggc attctcggag agtatctcga cctttttctc tttgcccttc acggtcatga
     3061 cctcgccttc gaggatgaag tggcggccgg gggtcgaaac gaatacctgc gccttttccc
     3121 cttccttgcc ggggagaaag accagttcga ccgaattata gttgagtgcg aagggcgaaa
     3181 gataggcgtt gtaggccctg ccggtgccat cgttgcccgc catagggatg cttcccttct
     3241 cgccataggc gtagtcgtcg gcgacgaccc gcgcgatgta cttgacgccg cggttcttga
     3301 gttcgttggc gacgagccag agttcctcca tcaccatcgt cgggtcgccc tgcgtcttca
     3361 tgtagagcgt cgccgtctcg ggcttatagt agaagcgggt cttgaaggtg tagttgcgtc
     3421 cgaggtgttt cagcgccgcg ccggtcgtga tgagtttcac attcgaggcg taagggaact
     3481 ccttgtcgcg attgagtgag aacgcctcct ttttcgccgt gatgtcataa aggacgatgc
     3541 ctgcgtcctc gggcgcgatg atgccgagat ccggcgcgaa cggctgctgg gtggtgtctg
     3601 cggctcccag aagaagagga aggatgatgg cgggaagaaa aacgagcgtt ctcatccggc
     3661 ggcgctacgg ggggagcggt gctcgacaag ccactgccgt atcttggtca cgatcatgtc
     3721 ggtcgcgacg acattgtcgc cgccctcggg caggacgagg tcggcc
//
LOCUS       DAOQUE010000265         3764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_27575_length_3764_cov_3.809113, whole genome shotgun sequence.
ACCESSION   DAOQUE010000265 DAOQUE010000000
VERSION     DAOQUE010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27575_length_3764_cov_3.809113"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="PKH10_08520"
     CDS             complement(<1..1483)
                     /locus_tag="PKH10_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT28207.1"
                     /translation="MTTLFLPQDLVISEVNDNEVILYRTADEVVGRIAGLPSRAIVVT
                     YEEQAPEVKSALRDRDWLVLVAFGKGFEHRPPNIFSVDGEKLSAYTLYLLVQLIEVID
                     SLRDEVIGNYHIGVDLSSEKDPQRLWDKILSFTRRATRAEAGTLYLMSRDRKSIFFIR
                     SQNEKLNRVDIERREIPFNRRSLVGFVCATGETLNIPDAYALPDDAPYSFNKQIDIML
                     GYRTHSVLTAPMKTPRGDVIGAVQLLNKHEIQSGYLPFSKYDELLLQSLASLSAVTVE
                     NNRLYEEIRGLFDSLVVSSVKAIEQRDPATKGHSVRVSCMMRKTLELLAAGDKDDFSD
                     DLLRGAEIASMLHDFGKVGIRENILTKANKLHADDRERVFWRTRYLRERLAHETTKGH
                     DHRKDIELIDRFLARLDAINTPAPLSDSDREILAETAARRLHLDGEELPVITVQEHRY
                     LSIERGNLTPEERTEMEQHAVKSWEILKAMQWPWELSQVPDHVL"
     gene            complement(1480..>3764)
                     /locus_tag="PKH10_08525"
     CDS             complement(1480..>3764)
                     /locus_tag="PKH10_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNT28208.1"
                     /translation="NLSVRNPYFFFSARRFFGDTGGTLFRVLARLAERSVTYEIADLE
                     GLRALSAGRQTLFYISNRSSIIEQFLVNVFLISRGIRAPSHSFGPRTLIFFTFRDLWR
                     LLVEWLKGGPPRRYAPKITALYLPVEDQEFFTHDPIWADFMSKIPAKEIAAVPVTTLW
                     DKVFREEKEERSWLIPFIGTRYLWSTFRELLLLLLGRRRLTVRLGVAHTIPKERYTPH
                     LYRKFYAILKGEKKNVVGAALRNWFEIRNETLLSLQASSELDKKKAARHLDEIATHYS
                     PYFTEAVSRLIGRLLSSVFSRILYRVEELDYLRRLAAVRNITLVFVPSHRSYFDYLLM
                     FHLLYREQVAIPLVVAGDNLDFFPLGGILRRLGAFFIRRRVRDDAAYQEVFRAYLRTI
                     VESGYHLEFFIEGGRTRSGKVRAPRTGVLSMLADIRKKSGRRLYIVPVSIAYEQLREV
                     RQYQTEATATKEPERKGFLRKFIGLIRGSAGPVSVSFGHPLYLPGGAADDFALEVAYR
                     IERNGQVGFSGLFAGFFLADNEVFAPALVRKMAHLVHLLGKSGRYRLAPALSHPELNV
                     PRVIERLRASGQIVESRRREHAYRLSREARAEFAYHRNTAAFALAPLFLALLDVPAPV
                     KAAVERYLEAVIQGYRTDDRLDDALVPGEAESWVLPLLCRFFLPSFELLAQITTLLRE
                     QPGPFESRSAAAQLIGRLLSRRRALFSTDEVFDALFYLEQRGAIDKLFAFVPETGENV
                     AAETRTIIAALKERGEETTR"
BASE COUNT          684 a         1078 c         1326 g          676 t
ORIGIN      
        1 cgagcacatg gtcggggacc tgcgacagtt cccagggcca ctgcatcgcc ttgaggatct
       61 cccaactctt gacggcgtgc tgttccatct cggtccgttc ctcgggggtc aggttaccgc
      121 gctcgatgga gagatagcga tgctcctgca cggtgatgac gggaagttcc tcgccgtcca
      181 gatgaaggcg gcgggcggcc gtttcggcga ggatctcgcg gtcgctgtcc gaaaggggcg
      241 ccggcgtgtt gatggcgtcg agccgcgcga ggaaccggtc gatgagttcg atgtctttgc
      301 gatggtcgtg gcccttcgtc gtttcgtggg cgagccgttc gcgcaggtaa cgggtgcgcc
      361 agaacacccg ttcgcggtcg tcggcgtgga gtttattcgc cttggtgagg atgttctcgc
      421 ggatgccgac cttgccgaaa tcatggagca tggaggcgat ctcggcgccg cgtaggagat
      481 cgtcggaaaa gtcgtccttg tccccggcgg caagcagttc gagcgtcttg cgcatcatgc
      541 aggagacgcg gaccgaatgg cccttcgtcg ccggatcgcg ctgttcgatg gccttgaccg
      601 aggagacgac gagcgaatcg aacagaccgc ggatctcctc gtacagccgg ttgttctcga
      661 cggtgaccgc cgagagcgag gcgagcgact gcaggaggag ttcgtcgtat ttggagaacg
      721 gcaggtagcc gctctgtatc tcatgcttgt tgagcagttg gacggcgccg atgacatcgc
      781 cgcgcggcgt cttcatgggg gcggtgagca ccgagtgggt gcggtacccg agcatgatgt
      841 ctatctgctt gttgaaggag tagggggcgt catccggcag ggcgtaggcg tcggggatgt
      901 tgagcgtttc gccggtcgcg cagacgaacc cgacgagact gcggcggttg aacggtatct
      961 cgcgccgttc gatgtcgacg cggttgagtt tctcgttctg cgaacggatg aagaagatgc
     1021 tcttgcggtc gcggctcatg agatagagcg tcccggcctc ggcgcgggtc gcccggcggg
     1081 tgaaggagag tatcttgtcc cagaggcgct ggggatcctt ttcggacgag aggtcgacgc
     1141 cgatgtggta gttcccgatg acctcgtcgc ggagcgaatc tatgacctcg atcaactgga
     1201 cgaggagata gagcgtgtag gcgctcagtt tctcgccgtc gaccgagaag atgttcggcg
     1261 gccgatgctc gaacccttta ccaaaggcga cgagcacgag ccagtcgcgg tcgcgcaggg
     1321 ccgatttcac ctccggcgcc tgctcctcgt aggtcaccac gatggcccgc gagggaagtc
     1381 cggctatccg gccgaccacc tcgtcggccg tgcggtagag gatgacctcg ttgtcgttca
     1441 cctcggaaat gacgaggtcc tgaggcagga acagcgtggt caccgcgtcg tctcctcccc
     1501 gcgctccttc agcgcggcaa tgatggtcct ggtctcggcc gcgacattct cgccggtttc
     1561 ggggacaaag gcaaataatt tatcgatagc cccccgttgt tcgaggtaga acagtgcgtc
     1621 gaacacctcg tcggtcgaga agagcgcgcg gcggcgggag agcaggcggc cgatcagttg
     1681 cgcggcggcg ctccggcttt cgaacgggcc gggctgttca cgcaacaggg tcgttatctg
     1741 ggcgagcagt tcgaacgacg gcagaaagaa gcggcagagc aggggaagca cccacgactc
     1801 ggcctcgccg ggcacgagcg cgtcgtcgag ccggtcgtcg gtgcggtagc cctggatgac
     1861 cgcctcgagg taccgttcga ccgccgcctt gaccggcgcg gggacatcga ggagcgcgag
     1921 aaaaagcggc gcgagcgcga aggcggcggt gttgcggtga taggcgaact cggcgcgcgc
     1981 ctcgcggctc aaccggtagg catgctcgcg gcggcgcgat tcgacgatct gtcccgacgc
     2041 gcgcaatcgc tcgatgaccc gcggcacatt gagttcgggg tgcgagagcg cgggggcgag
     2101 ccggtaccgc ccgcttttcc cgagcaggtg aacgaggtgt gccatcttcc ggacgagcgc
     2161 cggggcgaac acctcgttgt cggcgaggaa gaatccggcg aacagtcccg agaacccgac
     2221 ctgtccgttc cgctcgatac ggtaggccac ctcgagcgcg aagtcgtcgg cggctccgcc
     2281 ggggagatag agcgggtgac cgaacgatac agaaacgggg ccggcgcttc cccgtatgag
     2341 gccgatgaat ttgcgcagga agcccttccg ttccggttct ttggtcgcgg tcgcctcggt
     2401 ctggtactgc cggacctcgc gcaattgttc gtaggcgatg gagaccggga cgatgtagag
     2461 tcgccgaccg ctcttcttcc tgatgtcggc gagcatcgag aggacgccag tgcgcggcgc
     2521 ccggaccttg cccgatcggg tgcgtccccc ctcgatgaag aattcaaggt ggtagcccga
     2581 ttcgacaatg gtccgcagat aggcgcggaa gacctcctga taggccgcat cgtcgcgaac
     2641 gcgtcgccgg atgaagaagg cgccgagccg tcgcaggatg ccgccgagcg ggaagaaatc
     2701 gagattgtcc cccgccacga cgagcgggat ggcgacctgt tcgcggtaga ggaggtggaa
     2761 catgagcaga tagtcgaaat aactgcggtg cgacggcacg aacacgagcg tgatgttgcg
     2821 cacggcggcg agccgtcgca ggtagtcgag ctcctccacc cggtagagga tgcgtgagaa
     2881 gaccgacgaa agaagccgac cgatgagccg cgacaccgcc tcggtgaagt aggggctgta
     2941 gtgcgtcgct atctcgtcga ggtggcgtgc cgccttcttt ttgtccagtt cgctcgacgc
     3001 ctgcagagag aggagcgtct cgttgcgtat ctcgaaccag ttgcgcagag cggcgccgac
     3061 gacattcttc ttctccccct tcagtatcgc gtagaatttg cggtagaggt gcggggtgta
     3121 ccgctccttt ggtatcgtgt gggcgacccc gagccggacc gtcagccggc ggcggccaag
     3181 caggagcagg agcagttcgc ggaaggtgga ccacaggtag cgcgtcccga tgaaggggat
     3241 gagccaactg cgctcctcct tttcttcgcg aaagacctta tcccacagcg tcgtgaccgg
     3301 taccgccgcg atctccttgg cggggatctt cgacatgaag tcggcccata tcggatcgtg
     3361 ggtgaagaac tcctgatcct cgaccggcag gtagagcgcc gttatcttgg gagcgtaccg
     3421 ccgcggcggg cctcctttga gccactcgac cagaagccgc cacaggtccc ggaaggtgaa
     3481 aaagatgagc gtgcgggggc cgaacgaatg ggacggggcg cggatgccgc gtgatatgag
     3541 aaagacattc acgaggaact gttcgatgat ggaggagcgg ttggagatgt agaagagcgt
     3601 ctgcctgccg gccgacaggg cgcggagccc ttcgaggtcg gcgatctcgt aggtgaccga
     3661 ccgttcggcc aaccgggcga gcacgcgaaa gagcgtcccg ccggtgtcgc cgaagaagcg
     3721 gcgggccgaa aagaagaagt aggggttgcg tacgctcaaa ttac
//
LOCUS       DAOQUE010000266         3753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_27678_length_3753_cov_4.582477, whole genome shotgun sequence.
ACCESSION   DAOQUE010000266 DAOQUE010000000
VERSION     DAOQUE010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27678_length_3753_cov_4.582477"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1429
                     /gene="rmuC"
                     /locus_tag="PKH10_08530"
     CDS             170..1429
                     /gene="rmuC"
                     /locus_tag="PKH10_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNT28209.1"
                     /translation="MEALLILIGVVAGFAAGWFIAVRVATARATAEKEAMQNLMAAKE
                     SFFAQQIADLKAQHAAALAEMRDAFKALSADTLKEQAPEFLRLAGENFGKLQATAQGD
                     LAKRQEAIAGMLKPLEEQLKIYQQRLQQSEKDQATTLGEVKKQLETIGRQSETLTEVT
                     QKFRMVLSSSQARGKWGEETLRRVVEAAGMSAHCDFVEQMSGDEARPDLIIRLPGERV
                     IIVDAKVPDLDFLSALDLADAVQRAEALSRHAKKLRDTVKGLADRGYPAKFANALDHV
                     VLFMPAESLFSAALEADRDLIVWAAERKILLATPASLIALLRSVSVSWQQAQQAENAQ
                     IISRTAQELYERITVFTEHFDRIGKALAVANDAFGKAAGSYEKRVRPQGERLIELGVA
                     PRGDKRLADLSAAAFPAGSPDGTREGQ"
     gene            2362..>3753
                     /locus_tag="PKH10_08535"
     CDS             2362..>3753
                     /locus_tag="PKH10_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28210.1"
                     /translation="MKRSLFVICLMFSMLLGAAEDIGKETMDNIPLVSPFAIKESKPV
                     PLPASEIPALKPIPVTASDVSADLLVLYDAAIKADRKGKSDPRSASRAWGHVSSFKGA
                     NPFLDQANERKKMWGVYTKKQFIQKRYEKAKKNDKYGNLFPELVISQWEKVAAIEKDN
                     DYLDMAKKRIAAWKDFIERVNDYQKKKKSFAEQRDKDRKTLMKILPLSVVPENQKKDL
                     LKKYLVVYSPYYGVGDLDALFVELRNPVVAEAMKKLIDTPQFREEMEKGCDDKSPVSC
                     LIAAHYAERDRPAVATALYLTACRGGIGTACEKVATMAAGKDEKTTIEANWNACAWGF
                     AHSCLTLGGFVEAGKGIERSLALTAKLYEKACGGGLQEACERQKVVELAAKDEREKEE
                     QEARRVMESGDDAAGSEAPPLSSKAGTLDIGGETLNDIVLIIPMEYREEPLPVATEAA
                     PFTPEVSVDATTDP"
BASE COUNT          895 a          960 c         1130 g          768 t
ORIGIN      
        1 gtgaatcttg cggtcgaacc ggtagccttt gggaaggttg aactgatgat cggccacaaa
       61 agaaagaaat gcctttttct cggccttggc gctcaggttc tcctgaaata gttgcgcttt
      121 cgtgaccgcc tattagaatc agtaccacga attcggaggg aggttcctga tggaggcgct
      181 tttaatactc atcggcgtcg tggccggttt cgcggcgggc tggttcatcg ccgtccgggt
      241 cgcgacggcg cgggcgaccg ccgaaaaaga ggcgatgcag aacctcatgg cggccaagga
      301 atcgttcttc gcccagcaga tagccgatct caaggcccag cacgcggcgg cgctcgccga
      361 gatgcgtgac gccttcaagg cgctgtcggc cgatacgctc aaggaacagg cccccgaatt
      421 cctgcggctc gccggtgaga atttcgggaa actgcaggcg accgcgcagg gcgatctcgc
      481 caagcggcag gaggcgatcg cgggcatgct caaaccgctt gaagagcagt tgaagatata
      541 ccaacagcgg ctccagcaga gcgaaaagga tcaggcgacg acactcggcg aggtgaaaaa
      601 acaactcgag accatcggca ggcagagcga gacgctgacc gaggtgacgc agaaattccg
      661 catggtcctt tcatcgtcgc aggcccgcgg taaatggggt gaagagaccc tgcggcgcgt
      721 cgtcgaggcg gccgggatga gcgcccattg cgacttcgtg gagcagatga gcggcgacga
      781 ggcgcgcccc gacctcatca tccgcctgcc gggtgagcgg gtcatcatcg tggacgccaa
      841 ggttcccgat ctcgattttc tttcggcgct cgatcttgcc gacgccgtcc agcgcgccga
      901 ggcgctttcc cgtcatgcga agaaactacg cgacaccgtc aaggggctcg ccgaccgcgg
      961 atatcccgcc aaattcgcca acgcgctcga ccatgtcgtc ctgttcatgc cggccgagtc
     1021 gttgttcagc gcggcgctcg aggccgatcg cgacctcatc gtctgggcgg ccgagcggaa
     1081 gatactgctt gccactcccg cgtcgctcat cgcgcttttg cggtcggtca gcgtcagttg
     1141 gcagcaggcc cagcaggccg agaacgcgca gatcatcagc cggacggcgc aggaactcta
     1201 cgaacggata acggtattca ccgagcattt cgacaggata ggcaaggcgc tcgccgtggc
     1261 caacgatgcg ttcggtaagg cggcgggcag ttacgagaaa cgggtgcggc cgcagggtga
     1321 acggctgata gaactcggcg tcgcgccgcg cggcgataag cggctggccg atctttcggc
     1381 ggcggcgttc cccgccggat cgcctgacgg cacgcgggag ggccagtaga agagtgacat
     1441 tagaagagtg acattagaag attctttgcc tgcgatcctt aaaaagatat aatcagcttt
     1501 caggccccat ttttaaggac gatgaggata tcatccccta tgacttagga agggatcgaa
     1561 gacggagcgt gggatgatgg aaactgctcg ccgggttaag tctgcaatgt ctccaatcag
     1621 tgcgtactgg ccgtgccagc cgaagcctgc tcgaccggca ccccgagtcg tgatcgttgc
     1681 tcgaacgcac gcgtcgtcgg ccggagcacc gcaaagacat cgagcggcta taccatatcg
     1741 gacaacacct gttacgcata caatcgtgtc gatcagtcga gcggatgctg ggatgctggg
     1801 gcggaccatg tatatcgcat atatctgaga gcaagtgaaa cagcggcaat gccccttgct
     1861 cttgatatgg cttgtccgtt ttacgaggat ttttcgtggg atgcgaccct tgccatatac
     1921 aatgatatcg ggtgtgcatc ggcggcccaa tcatggtgtg acgacaactt tagcgggaca
     1981 acgatctata ctgccccggc tgatagttgg tactacatcg ttgtggacgg agcaaccatt
     2041 tttgaagatg gaggagacta ttcttttaca gttaaactgc tggattgcgt agctaccgac
     2101 tgtaattgtc cctagcgcat caaaaaacga aaaacgaaca tctggccgtt ttttataaac
     2161 gcgaagatgg gggtgtattc cgacgataga ggagagactg agacaccgcc cccttcatgc
     2221 gccataaatg tcccgaatat tttttgcata gtattaggaa aaagatataa tcgaccgtcc
     2281 gcaccttttt gggagggtgt ctggctttgc aacgaactca agacgatctg attcgcgaga
     2341 acgattaaat aggagtttaa aatgaaaaga tctttgttcg tcatctgtct gatgttctcc
     2401 atgttgcttg gtgccgccga ggacatcggt aaggaaacca tggacaacat cccgcttgtt
     2461 tccccattcg ctatcaagga atcgaagccg gtgccactgc cggcatccga gatacctgca
     2521 cttaagccaa taccggtcac ggcgtcggac gtgagcgccg atctgctcgt tctttatgat
     2581 gccgccatta aggccgatcg gaagggtaag agtgaccccc gcagtgcaag ccgcgcatgg
     2641 ggtcatgtat cctcatttaa aggggcgaac ccgttccttg atcaggcgaa cgaacggaag
     2701 aagatgtggg gcgtctacac aaaaaaacag tttattcaga aacgatacga aaaagcgaaa
     2761 aaaaatgaca agtatgggaa tctcttccct gagctggtca tttctcagtg ggaaaaggtc
     2821 gcggcgatag agaaggacaa cgattacctc gatatggcga aaaaacggat agcggcatgg
     2881 aaggatttta tagaaagggt gaatgattac cagaaaaaga aaaagtcctt tgccgaacag
     2941 cgcgataaag accgcaagac acttatgaag atactgcctc tttctgttgt gccggagaac
     3001 cagaaaaaag acctgttgaa aaaatatctg gtcgtctata gtccttacta cggcgtgggt
     3061 gatcttgacg cgctattcgt tgaattgcgt aatcccgtgg ttgctgaggc gatgaagaaa
     3121 ttaatagata ctccccaatt ccgggaggag atggaaaagg ggtgtgacga taagagcccg
     3181 gtgtcgtgtc tgatagcggc tcattatgcg gaacgcgatc gaccggcggt tgcgaccgct
     3241 ctctacctga cagcctgtcg tggtggcata gggaccgcct gtgagaaggt ggcgaccatg
     3301 gccgctggca aggacgaaaa gacaacgatc gaggccaatt ggaacgcctg tgcgtggggt
     3361 tttgcgcatt cctgtctcac cctgggcggt ttcgtcgagg ccggcaaggg aatagaacgc
     3421 tctcttgctc ttacggcaaa actttacgaa aaggcttgcg gtgggggatt gcaagaggcg
     3481 tgtgagcgtc agaaggtagt agaacttgcg gcaaaggacg agcgtgagaa ggaagaacaa
     3541 gaggcgcgtc gcgttatgga atcgggggat gatgccgccg gatcggaggc gccgcctctt
     3601 tcctcaaagg cgggtacgct tgatatcggt ggcgaaacgc tgaatgacat cgttttgatt
     3661 atacccatgg agtatcgcga agagccctta ccggtcgcga ccgaagcggc gccctttacg
     3721 ccggaggtct cggtggatgc aacgacggac ccc
//
LOCUS       DAOQUE010000267         3724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_28016_length_3724_cov_3.928046, whole genome shotgun sequence.
ACCESSION   DAOQUE010000267 DAOQUE010000000
VERSION     DAOQUE010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28016_length_3724_cov_3.928046"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="PKH10_08540"
     CDS             <1..1621
                     /locus_tag="PKH10_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HNT28211.1"
                     /translation="WQIFFSSLLGGFLGILFLIPFRKYFVRDMHGKLPFPEATATTEI
                     LMTGEKGGAQAKLLVVSGLIGGFFDFLFTYLKLWAEEFTSRVLPYGEMLADKAKMVFK
                     FNVSALIFSFGFLVGLRFSLIIVAGSLLSWFVFVPLINEIGVLAVAAGAPSNPFAAMP
                     PELIFKNYVRPIGIGAIAMAGIIGIIKSGGVIGSAFKLAVGVGGKASGEAQAVRTDRD
                     LKMSFVLLVIGLTLVAVFLFLLFGVKVTFIQAVVALITIFVISFLFTTVAANAIAIVG
                     TNPVSGMTLMTLILSSVILVWVGLSGWQGMIAALIIGGIVCTALSMAGGFITDLKVGY
                     WLGTSPYKQQTYKFLGTLVAAATVGIVIFVLSEVFGFVQTAEHPNPMVAPQANAMAAI
                     IEPLMSGTGAPWMLLAIGAVISILMNWLKISPLAFALGMFIPLSLNTPLVVGGLLNHY
                     FNKNADEKLAKARNDRGILIASGFIAGAALFGVIGAVILFITGSEESLMLVNWGEYVH
                     GAVISETLAFVAFACLIGYFIWESFKAKAED"
     gene            complement(1618..>3724)
                     /locus_tag="PKH10_08545"
     CDS             complement(1618..>3724)
                     /locus_tag="PKH10_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT28212.1"
                     /translation="EVVSDQSTFINNSLHELKLHLTLGALFAAIAVFLFLGHIRSTLI
                     AALAIPTSLIATFTFMYLMGFTLNNMSLLGLTLAVGIVIDDAIVVLENIFRHIEQYGK
                     NAFDAAIDGLREIGLAVTATTTSLLVIFLPIAFMQGMSGRFFYEFGLTTAFAIAVSLL
                     ISFTLTPMLASRFLGAKPHDERSLIERFWKWLDDRYAALVRFSLHHRWVIGLVAVAVI
                     ASAVPIAKRLGTDFMPLDDRGEIQVELQLPSGVTLESARAAVDAIERDLRTVRGVAKT
                     LMRIGSSVSDDVTGAKIYVALVPLEEREFSQFEVQRDIRRILKRYPEIISSASAIGGS
                     FGGGRSATFQYDIVGPDLDRLIAYSDAIVAKLRDQAGFADVSTSLVARKPEARIVIDR
                     LKAADLGVSVADIATTLRAFVGGEIVTKYREGSEQYDVWLRLDRRFRDDASALSTLTV
                     PSAKGVPVPLSQITQVEYGKSPSVIDRFNRQRRVSLYANLDGLDIGTATATIDRMVKE
                     LDMPLGYAPVKIGRSKVMAESMQNLLMAFALAFIFIYIVLAAQFESFVHPITILLSLP
                     LTIPFALLSLLMTGETINLYSLLGVFMLFGIVKKNGILQVDYANTLRDRGEPLEKAIV
                     EANKARLRPILMTTMTLVAGMIPIALGTGPGAAARASMAKSIVGGQILSLLITLLVVP
                     VGYHLFESVKMRLGFGKRD"
BASE COUNT          689 a         1130 c         1176 g          729 t
ORIGIN      
        1 ctggcagata ttcttctcgt cgctcctcgg cggcttcctc ggcatcctct tcctcatccc
       61 cttccgcaag tacttcgtgc gcgacatgca cggcaaactc cccttccccg aagcgaccgc
      121 caccaccgag atcctcatga ccggtgaaaa gggcggcgcg caggcgaaac tgctggtcgt
      181 cagcggcctc atcggcggtt tcttcgactt tctcttcacc tatctgaaac tctgggccga
      241 ggaattcacc tcgcgcgtcc tgccctacgg cgagatgctc gccgacaagg cgaagatggt
      301 cttcaagttc aatgtgagcg cgcttatatt cagcttcggc ttcctcgtcg gtctgcgctt
      361 ctcgctgatc atcgtggcgg gttcgctcct gtcgtggttc gtattcgtcc cgctcatcaa
      421 cgagatcggc gtgctggccg tcgcggccgg cgcaccgagc aaccccttcg ccgcgatgcc
      481 tcccgaactg atcttcaaga actatgtccg ccccatcggc atcggcgcga tcgcgatggc
      541 cggcatcatc ggcatcatca aatcgggcgg cgtgatcggg tcggcattca aattggcggt
      601 cggcgtcggc ggcaaggcct ccggcgaggc gcaggcggtc cgtaccgacc gcgatctcaa
      661 gatgtcgttc gtgctgctgg tcatcggact cacgctcgtc gcggtgttcc tgttcctcct
      721 gttcggcgtc aaggtgacct tcattcaggc ggtcgtcgcg ctcatcacca ttttcgtgat
      781 atcgttcctc ttcaccaccg tcgcggccaa cgccatcgcg atcgtcggca ccaacccggt
      841 ctccggcatg acgctgatga cgctcatcct ttcctccgtt atactcgtct gggtcggcct
      901 cagcggctgg caggggatga tcgcggcgct catcatcggc ggcatcgtct gcaccgcgct
      961 ctccatggcg ggcggtttca tcaccgatct caaggtgggc tactggctcg gtacgagccc
     1021 ctataaacag cagacctaca agttcctcgg gacgctcgtg gcggccgcca ccgtcggcat
     1081 cgtcatcttc gttctgagcg aggtattcgg cttcgtgcag accgccgaac accccaatcc
     1141 aatggtcgcg ccgcaggcga acgccatggc ggcgatcatc gagcccctga tgtcgggcac
     1201 cggcgctccg tggatgctct tggcgatcgg cgcggtcatt tcgatactca tgaactggct
     1261 caagatatcg ccgctcgcct tcgcgctcgg catgttcatt ccgctgtcgc tcaatacccc
     1321 gctcgtggtc ggcggcctgc tcaaccatta ctttaacaag aacgccgacg aaaaactggc
     1381 caaggcgcgc aacgaccgcg gcatactcat cgcctccggc ttcatcgccg gcgcggcact
     1441 cttcggcgtc atcggcgcgg tgatcctgtt catcaccggc agcgaagagt cactgatgct
     1501 cgtgaactgg ggagagtatg tccacggcgc ggtgatatcc gagacgctgg cgttcgtcgc
     1561 gttcgcctgc ctcatcggct acttcatctg ggagtcgttc aaggcgaagg cggaggatta
     1621 atcccgcttc ccgaaaccga gccgcatctt caccgattcg aagagatgat accccaccgg
     1681 caccaccaac agcgtgatca gcagcgataa gatctgtccg ccgacgatgc ttttggccat
     1741 cgaggcgcgc gccgcggcgc cgggacctgt cccgagcgcg atcggtatca tgccggcgac
     1801 cagagtcatc gtggtcatca ggatgggacg gagccgcgcc ttgttggcct cgacgatcgc
     1861 cttctcgagc ggctcgccgc ggtcccggag cgtattggcg tagtcgacct gcaggatgcc
     1921 gttctttttg acgatgccga acagcatgaa gacgccgagc aggctgtaga ggttgatcgt
     1981 ttcgccggtc atcagcagag aaaggagcgc gaagggtatg gtgagcggca gggagagcag
     2041 tatcgtgatc gggtggacga aactttcgaa ctgcgccgcg aggacgatgt agatgaagat
     2101 gaaggcgagc gcaaaggcca tgaggagatt ctgcatcgac tcggccatca ccttcgaccg
     2161 gccgatcttg accggagcgt agccgagcgg catatcgagt tccttgacca tccggtctat
     2221 cgttgcggtg gcggtgccga tgtccagacc gtcgaggttc gcgtagagcg aaacgcgacg
     2281 ctggcggttg aagcggtcga tgaccgatgg gctcttgccg tattcgacct gcgtgatctg
     2341 tgagagcggc accggcaccc ctttggccga cggaacggtg agtgtgctga gtgcggaggc
     2401 gtcgtcgcgg aagcgtctgt cgagccgcag ccagacatcg tactgctcag aaccttcgcg
     2461 gtatttggtg acgatctcgc cgccgacaaa ggcgcggagt gtcgttgcta tgtcggcgac
     2521 actgaccccc agatcggccg ccttgagccg gtcgatgacg atgcgcgcct cgggcttgcg
     2581 ggccaccagc gaggtggata catcggcgaa gcccgcctgg tcgcgcagtt tcgcgacgat
     2641 cgcgtcggag tatgcgatca gccggtcgag atcgggtccg acgatatcgt actggaaggt
     2701 cgccgatcgt ccgccgccga aactgccgcc gatggcgctc gccgacgaaa tgatctctgg
     2761 atagcgcttg agaatgcgcc ggatgtcgcg ctgtacctcg aactgcgaga attcccgttc
     2821 ctcgagcggc acgagagcca catagatctt tgcgccggtc acatcgtcgg acaccgaact
     2881 gccgatccgc atgagcgtct tggcgacccc ccgcaccgtc cgaagatccc gttctatagc
     2941 gtcgaccgcc gcgcgggcac tttcgagcgt cacgcctgac ggcaactgca actccacctg
     3001 tatctcgccg cggtcgtcca gcggcatgaa gtcggtcccg agccgtttgg cgatggggac
     3061 cgccgaggcg atgacggcga cggcgacaag gccgatgacc cagcggtgat gcagggagaa
     3121 acggacgagc gcggcgtagc ggtcgtcgag ccatttccag aaccgttcga tgagcgaccg
     3181 ttcgtcgtgt ggtttggcgc ccagaaagcg cgaagcaagc atcggagtga gggtgaagga
     3241 gatgaggagc gacaccgcga tggcgaaggc ggtggtgagg ccgaattcgt agaagaaacg
     3301 gcccgacatc ccttgcatga aggcgatcgg caggaagatg acaagcagcg aggtggtggt
     3361 cgcggtgacc gcgagcccga tctccctgag cccgtcgatg gcggcgtcga aggcgttctt
     3421 gccgtattgc tcgatatgac ggaaaatgtt ctccaatacg acgatcgcgt cgtcgatgac
     3481 gatgccgacc gcgagcgtga gcccgagaag agacatgtta ttgagcgtga aacccatcag
     3541 atacatgaaa gtgaaggtgg cgataagcga ggtcggtatc gcgagggcgg cgatgagggt
     3601 ggatcggata tgtccgagaa agaggaagac ggcgatggcg gcgaacagcg cgccgagcgt
     3661 gaggtgcagt ttgagttcgt ggagcgaatt gttgatgaag gtcgactggt cggagaccac
     3721 ctcg
//
LOCUS       DAOQUE010000268         3718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_28082_length_3718_cov_4.198744, whole genome shotgun sequence.
ACCESSION   DAOQUE010000268 DAOQUE010000000
VERSION     DAOQUE010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28082_length_3718_cov_4.198744"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PKH10_08550"
     CDS             <1..835
                     /locus_tag="PKH10_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28213.1"
                     /translation="ICFICAWMISNKHDDATAAEKSGTPQKTPLQIFAEVWKESAFQK
                     LVLFLVLTLGVRLVFTHQTMVMPKYYIRTLYNDFELGLVNSFNPIIIVVGLILFIPII
                     NKFNIVKLLVVGMTISALSLLFLAVPGEWILAIPGIQNLNQAYLFIIVAQIFVFAIGE
                     LIFSPRFVEYVASVAPKDKVASYMGLSALPMFIARPINGFVSGMLISSYAYDGIRAKI
                     DTGNVEYMQSPEWMWMIYFILAAISPIAILLWRNKVTSNPTDADSAGKKEEPAAEEAA
                     H"
     gene            849..1490
                     /locus_tag="PKH10_08555"
     CDS             849..1490
                     /locus_tag="PKH10_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT28214.1"
                     /translation="MKQYLPIIKDGLILMAFSTVFAVICYFGFNALKGQGVKTDPIVA
                     AIIQNDIDQMKKLIESESGRVNNLDEQGRTPLMWAAYVNFKDGALVAKNEEKRVEATR
                     LLLEKSADPNLKDKDGWTALMWASWSNFPKVVEALLNAGADHAVADKRGHTALMIAAL
                     RGNAAVAKLLVERGADRAAKNVDGRTAADIVKEMSPKHPDRKDAYNEIATLSQ"
     gene            1487..1780
                     /locus_tag="PKH10_08560"
     CDS             1487..1780
                     /locus_tag="PKH10_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28215.1"
                     /translation="MSKQSLNRDILFPGTGPASARSVWLLLAIMFALLPLIDLPRPAA
                     ASCGALSLVSAGIALRRWILFSAIAAIVTFIGCCLEIEVPYRWFHDLVRVVLG"
     gene            1777..2355
                     /locus_tag="PKH10_08565"
     CDS             1777..2355
                     /locus_tag="PKH10_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28216.1"
                     /translation="MNEGTMLRRIRAFEAYLQIELTRKWALALLFLFFLLPRSGLLFL
                     AVLFFAASLLPKDVQSRRLSLYLALPFTRTGLFWCSFGTGLLLMLLGALISGAIGIDP
                     DWASKLISMLIFFVAYFSISLIGAVAGRDTLTLPILALFTEITAGATLRVEHPFRAFS
                     PFYQEDLLYSGLVALLLLAAAFTHYQIVVKRQ"
     gene            2352..3152
                     /locus_tag="PKH10_08570"
     CDS             2352..3152
                     /locus_tag="PKH10_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT28217.1"
                     /translation="MTLIVEKVSKRFHDRVGLTEVSFMTGPGEIVAVTGPNGAGKTTL
                     MRVACGELPPSEGYIRLDGSPVRSFRRDAVAFTGDQRHVFTGFTGYDYLRVWKLLYAR
                     FDDALFASFVTRFSWDLLSRVDHMPLGVRSLFLTLGALACRPQILLIDEPFQHIAPIE
                     RDNLCMILRSFADEHRSTVLLASTELYEQEPIIDRLVVLKEGKLLFSGSLEKARQTHR
                     VVPYAEDAEGLLIIGPVLAEHLVFGDEDLGREPTMREVVTGYLNGYSD"
     gene            complement(3149..>3718)
                     /locus_tag="PKH10_08575"
     CDS             complement(3149..>3718)
                     /locus_tag="PKH10_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNT28218.1"
                     /translation="LTGSIGVIIGKMYTPDMWKKIGVTFDSDQIGKNARLFSSLTRLD
                     ADQTTLLRRMLDSIYDTFVSRAAAGRKKNPEEIEPFAKGRVWTGEDALAHGLVDELGG
                     YTAAFAEARKRLGLQPGAMVSLKFYPPEPNFFDYLLDRDSDDEVAATTGLLTDLSAGA
                     RKLFSLLMMVEKLERRENFELSTEPVDIR"
BASE COUNT          677 a         1204 c         1098 g          739 t
ORIGIN      
        1 catctgcttt atctgcgcat ggatgatatc gaacaagcac gacgacgcga ccgccgccga
       61 gaaatcgggg accccgcaga agacgccgct ccagatattc gccgaggtgt ggaaagagag
      121 cgcgttccag aaactggtgc tcttcctcgt gctgacgctc ggcgtccggc tcgtattcac
      181 ccaccagacg atggtcatgc ccaaatacta catccgcacc ctctacaacg atttcgaact
      241 cgggctggtg aactccttca acccgatcat catcgtggtc ggcctgatac tcttcatccc
      301 gatcatcaac aaattcaaca tcgtgaaact gctggtcgtc ggcatgacga tatcggcgct
      361 ctcgctcctg ttcctcgccg tgccgggaga gtggatactg gccattccgg ggatacagaa
      421 cctcaatcag gcctatctct tcatcattgt tgcgcagata ttcgtcttcg cgatcggcga
      481 actgatattc tcgccgcgtt tcgtcgagta tgtcgcctcg gtcgcgccga aggacaaggt
      541 cgcctcctac atggggctgt cggcgctccc gatgttcatc gcgcgcccca tcaacggctt
      601 cgtgagcggg atgctgatat cgagttatgc ctatgacggc atccgcgcca agatagatac
      661 cggcaatgtc gagtacatgc agagccccga atggatgtgg atgatctact tcatcctcgc
      721 ggcgataagc cccatcgcga tactgctgtg gcgcaacaag gtcacctcga acccgaccga
      781 cgccgacagc gccgggaaga aggaagaacc cgccgccgaa gaagcggctc actagaggga
      841 ggcgtacgat gaaacaatat ctgcccatca tcaaggacgg cctcatactg atggcgttca
      901 gcacggtgtt cgccgtcatc tgctatttcg gtttcaacgc gctcaagggt cagggggtca
      961 agaccgatcc gatcgtcgcg gccatcatcc agaacgacat cgaccagatg aagaaactca
     1021 tcgagtcgga aagcggacgg gtcaacaacc tcgatgagca ggggcgcacc ccgctcatgt
     1081 gggccgccta tgtgaacttc aaggatggcg cgctcgtcgc gaagaacgag gagaaacggg
     1141 tcgaggcgac gagactcctt ctcgagaaaa gcgccgaccc gaatctcaag gacaaggacg
     1201 gctggacggc gctcatgtgg gcgtcgtgga gcaacttccc caaggtcgtc gaggcgctcc
     1261 tgaacgccgg tgccgaccac gccgtcgccg acaagcgggg ccacacggcg ctcatgatag
     1321 cggcgctccg cggcaatgcg gcggtcgcga aactgcttgt cgagcgcggc gccgaccggg
     1381 ccgcgaagaa tgtcgacggc aggacggcgg ccgacatcgt gaaggagatg tcgccgaaac
     1441 atcccgaccg caaggacgca tataacgaga tagcgacgct ctcgcaatga gcaagcagtc
     1501 cctcaaccgc gacatcctgt ttcccggcac tggtcccgcg tcggcccgga gcgtatggct
     1561 cctgctcgcg atcatgttcg cgctcctgcc gctcatcgac ctgccgcgcc cggcggccgc
     1621 gtcctgcggc gccctgtcgc tcgtatcggc cggcatcgcg ctgcgccgct ggatactctt
     1681 ttccgccatc gcggccatag tgaccttcat cggctgttgc ctcgagatcg aggtcccgta
     1741 ccgctggttc catgaccttg tcagggtggt gctcggatga acgaagggac gatgctccgc
     1801 aggatccgtg cctttgaggc gtacctgcag atagaattga cgcggaaatg ggcgctcgcg
     1861 ctcctcttcc tgttcttcct tctgccgagg agcggcctcc tgttcctcgc ggtcctcttc
     1921 ttcgccgcct cgctcctgcc gaaagatgtg cagtcgcgcc ggctctcgct ctacctcgcc
     1981 ctccccttca cccgcacggg acttttctgg tgctcgttcg ggaccggtct tctgctcatg
     2041 ctcctcggcg cgctcatcag cggcgcgata gggatcgatc ccgactgggc gtcaaaactc
     2101 atctcgatgc ttatcttctt cgtcgcctat ttctcgatat cgctcatcgg cgcggtcgcg
     2161 ggacgtgata ccctcacgct cccgatactc gcgctcttca ccgagataac ggcgggagcg
     2221 acactaaggg tcgaacaccc gttccgcgcc ttcagcccct tctatcagga ggacctgctc
     2281 tactcaggtc tggtggcgct cctcctgctt gcggcggcct tcacccatta ccagatcgtg
     2341 gtgaaacgac aatgacgctt atcgttgaaa aggtatcgaa acgcttccat gaccgggtcg
     2401 ggctcaccga ggtctccttc atgaccggtc ccggcgagat cgtcgcggtc accggcccga
     2461 acggcgcggg gaagaccacg cttatgcgcg tggcctgcgg cgaactcccg ccctccgaag
     2521 ggtatatccg tctcgacggc agccccgtcc gatcgttccg ccgcgacgcg gtcgccttca
     2581 ccggggacca gcgccatgtc ttcaccgggt tcaccggtta cgattacctg cgggtctgga
     2641 aactgctcta tgcgcggttc gacgacgcgc tgttcgcttc gttcgtcacc cggttctcat
     2701 gggatctgct ctcgcgggtg gaccacatgc cgctcggcgt ccggtcgctc ttcctcacgc
     2761 tcggcgcgct cgcctgccgc ccccagatac tgcttatcga cgagccgttc cagcatatcg
     2821 cgccgataga gcgcgacaac ctctgcatga tcctgcggtc attcgccgac gaacaccgat
     2881 cgacggtgct gctcgcctcg accgaactct acgagcagga accgatcatc gaccggctcg
     2941 tggtgctcaa ggaagggaaa ctgctgttca gcggcagtct cgaaaaggcg cggcagaccc
     3001 accgcgtcgt cccgtacgcc gaagacgccg aagggttgct catcatcggc ccggtgcttg
     3061 cggagcatct cgtgttcggc gacgaggatc tcggtcgcga gccgaccatg agagaggtcg
     3121 tgaccggtta tctgaacggg tacagcgact agcggatatc gaccggttcc gtcgaaagtt
     3181 cgaagttctc gcgccgttcc agtttttcca ccatcatcag cagagaaaac agtttccggg
     3241 cgcccgccga caggtcggtc agtagtccgg tcgtcgccgc gacctcgtcg tcgctgtcgc
     3301 ggtcgagcag gtagtcgaag aagttaggtt cgggcgggta gaacttgagc gagaccatgg
     3361 cgcccggctg gaggccgagc cgtttccgcg cctcggcgaa ggccgccgtg tagccgccca
     3421 gttcgtcgac gagcccgtgg gcgagcgcgt cctcgccggt ccagacgcgg cccttggcga
     3481 acggctctat ctcctcgggg ttcttcttgc gcccggcggc ggcgcggctc acgaaggtgt
     3541 cgtatatgga atcgagcatc cggcgcagga gcgtcgtctg gtcggcgtcg agtctggtga
     3601 gcgacgaaaa gagccgggcg ttcttcccga tctggtcgct gtcgaaggtc acccctatct
     3661 ttttccacat gtccggcgtg tacatcttgc cgatgatgac cccgatgctg ccggtgag
//
LOCUS       DAOQUE010000269         3712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_28140_length_3712_cov_5.129614, whole genome shotgun sequence.
ACCESSION   DAOQUE010000269 DAOQUE010000000
VERSION     DAOQUE010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28140_length_3712_cov_5.129614"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..2413
                     /locus_tag="PKH10_08580"
     CDS             53..2413
                     /locus_tag="PKH10_08580"
                     /inference="COORDINATES: protein motif:HMM:NF025316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HNT28219.1"
                     /translation="MAEYRPVGRYDHAMTTDEAHGKVMLFGGKGDGEQELNDLWEWNG
                     ATWTQKLPPISSLPANLSGGVITYDESRDSIVLLSSYYTQDEVGYHNFTNTWEWLSGK
                     WALRNPPTIPTFSSGSMMYDGANNEAVLFSYSETWGWNGINWSKKAPSSTPTGNGVLV
                     YDQTREKGLLYTGSETWEWDGYDDSWAKKTTVNIPSASTGFAMAYDAERDKVVLFGGG
                     YSEQYFDPACGCWQTTTTLLEETWEWDGTDWTKMNPSVSPSPRNGSLMIYDSVRRELM
                     LFGGSGAGGFFPSDAWKWDGTEWQQIFPADMPSGPSGMASITYDSISGRTISLSGQTL
                     WELSGGATSRAAQIFSVALNTSGISAGTISGVSARFYAGGIGFPDDAETYGAELRIWD
                     EGRWKTKSTNDADPGTPLPLAWSTASDTEWNSLTSEESQEQLTRLFFGDRRSLNFAVT
                     PVAPNGIGSDMGELVVDYAEVVVSYIVDPDASDLCGDGDVQAGEECDDGNSINEDDCK
                     NDCTFNVCRDGSVRPGVEECDDGNEEENDSCKNDCTRNFCGDGVTRSGFEECDDGNEE
                     DKDTCSNSCIASFCGDGIINMSSFEVPEEVESFEWGIPADAENGGEPWEINASDKYDG
                     EYSSQSHRELPMGSVSIFTLYRGSDDEVCFYLKGRSDQENYFTFEVDAEEVLRTSGAS
                     AYEEWQQVCHLVDPGMHYLRFSYVKPIESTIENDGYHIDHIRLHKSFTFIGTEPCDDG
                     NDSNEDDCTNICRPPLCGDGFVWLGYEECDDGNFIDGDGCSGCVIN"
     gene            complement(2429..>3712)
                     /gene="gcvPB"
                     /locus_tag="PKH10_08585"
     CDS             complement(2429..>3712)
                     /gene="gcvPB"
                     /locus_tag="PKH10_08585"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNT28220.1"
                     /translation="HYTRLSTYNFSIDHTFYPLGSCTMKYNPKINEKMARLAAFANLH
                     PETPLGYAQGTLKMLFDLQHMLAEVSGFAEVSLNPAAGAHGEYTGLGVIRKYHQKRGD
                     TKRKKVIIPDSAHGTNPASCAINGFEAVVLESGPDGKICLDCLKGMLNDEVAAIMLTN
                     PNTLGVFETDICAITKACHDAGALVYGDGANLNAILGVARPGDMGIDVMHFNLHKTFS
                     TPHGGGGPGSGPVGVAKHLVEFLPNPVVRKEGDRYVFALLKDSMGRVRSFYGNVGIAL
                     RAYVYLLELGKEHIAEVAEQAVLNANYIKAKLKDIYNVKYPNDTLHEAVFDDSKLPNH
                     VTTMDVAKGLIDRGYHPPTVYFPLIVHGALMVEPTETESKETMDGFIDALKDIAEKAA
                     SDPQSILNAPTKTYIKRPDETLAARKPVLKYGGEK"
BASE COUNT          849 a          881 c         1101 g          881 t
ORIGIN      
        1 cctcggaaat cagatgtggg aatggagcgg cttcaattgg gaggaggtcg tgatggcaga
       61 gtaccggcca gtgggtcgct acgatcatgc catgacgact gacgaagcac atggcaaagt
      121 tatgctcttt ggcgggaaag gagatggaga gcaagagttg aatgatctat gggagtggaa
      181 cggggcgact tggacacaaa aattaccgcc catcagctcg ttgccggcaa atcttagcgg
      241 cggggtcatc acgtacgatg aatctcggga cagcatagtt ctcttgagca gttattacac
      301 ccaggatgaa gtaggatatc ataatttcac caatacatgg gaatggcttt caggaaaatg
      361 ggctcttaga aatccaccga ccatcccgac cttttctagc gggagtatga tgtatgacgg
      421 ggcaaacaac gaggccgttc ttttcagtta ctcggaaaca tggggatgga acggcatcaa
      481 ttggtcaaag aaagcaccct cttcgacacc aaccgggaac ggagtgttgg tatatgatca
      541 aacccgcgag aaggggctct tgtacaccgg gtccgaaaca tgggaatggg atggttatga
      601 tgatagttgg gcaaagaaaa ctaccgtgaa tattccatcc gcatcaaccg gctttgcaat
      661 ggcctatgac gccgaacgtg acaaagttgt tctgttcggc ggaggttatt ctgaacaata
      721 ttttgacccc gcttgtggct gttggcaaac aacaaccact cttttggagg aaacctggga
      781 atgggacgga accgattgga caaagatgaa tccgtcggtc agcccctctc ctcgaaacgg
      841 ctctttgatg atctatgact ctgtacgacg ggaactaatg ctctttgggg gctctggcgc
      901 aggaggtttt tttcctagtg atgcttggaa atgggatggg acagagtggc aacagatatt
      961 tcccgccgat atgccgtcag ggccatcagg gatggcatca atcacgtatg atagcattag
     1021 tggaagaacg atctcgctct ccgggcagac gttatgggaa ttaagtggtg gtgctacttc
     1081 tcgtgcggcg cagatcttta gtgtcgccct gaacacctcc ggtatttctg ccggaaccat
     1141 ctccggtgtt tcagccagat tctacgctgg tgggattggt ttccccgatg atgcggagac
     1201 ctatggtgct gaattgagaa tatgggatga agggcgatgg aaaacgaaat ccacgaacga
     1261 cgccgacccg gggacgccgt taccattagc atggtcaaca gcaagcgata ccgaatggaa
     1321 ttctctcacc agcgaggaat cgcaggaaca acttacccgc cttttctttg gcgatcgcag
     1381 atctctcaat tttgccgtaa caccggttgc gccgaacggg atcgggtccg acatgggaga
     1441 gctcgttgtt gattatgcgg aagttgtcgt cagttacatt gttgatccgg atgccagcga
     1501 cctttgtggc gatggcgatg ttcaggcagg tgaagaatgc gatgacggca acagcatcaa
     1561 tgaggatgac tgcaaaaacg attgcacttt caatgtgtgc agggacgggt ctgttcgccc
     1621 cggggttgaa gaatgtgacg atggcaatga agaagagaat gattcctgca aaaacgattg
     1681 cacccgtaat ttctgtgggg acggggtaac acgatccgga ttcgaggagt gtgatgacgg
     1741 gaacgaagaa gacaaagata cctgctctaa ttcctgtata gcctcttttt gcggcgatgg
     1801 catcatcaac atgtcgtctt ttgaggttcc tgaagaggta gagtcttttg agtggggaat
     1861 ccctgccgat gcagaaaatg gtggtgagcc ttgggaaata aatgctagcg acaaatatga
     1921 cggagaatat tcttcgcaaa gtcaccgtga attgcctatg ggctccgtga gcatcttcac
     1981 cttatatagg ggttctgatg atgaagtctg cttctacctg aaaggccgat ctgatcaaga
     2041 aaactatttt acttttgaag ttgacgccga agaagtgctg agaaccagcg gggcctccgc
     2101 gtatgaggaa tggcaacagg tgtgccatct ggtcgatccc ggcatgcact atctgcgatt
     2161 ttcgtatgtc aagccgatag aaagcacgat agaaaacgac gggtatcata tcgatcacat
     2221 aagattgcat aagagtttta cctttattgg tacggaacct tgtgacgatg gtaacgacag
     2281 taacgaggat gattgcacca atatttgtcg gccaccgctg tgtggtgacg gcttcgtttg
     2341 gctcggttac gaagaatgcg atgatggaaa tttcattgac ggcgacggct gctctggttg
     2401 tgtaataaac tgatttcctg tttttgcctt atttttcgcc accgtatttg agcaccggct
     2461 tccgggccgc gagcgtttcg tcgggccgtt tgatgtaggt cttggtgggg gcgttcagta
     2521 tcgactgcgg atcgcttgcc gccttctcgg cgatatcctt cagcgcgtca ataaagccgt
     2581 ccatcgtttc tttgctctcg gtctcggtcg gctcgaccat gagcgcgccg tggacgatga
     2641 gcgggaaata gaccgtcggc gggtggtagc cgcggtcgat gagccccttg gcgacatcca
     2701 tcgtcgtgac atggttgggt agtttggaat cgtcgaacac cgcctcgtgc agggtatcgt
     2761 tcggatactt aacattgtaa atatccttga gtttcgcctt gatataattc gcgttgagaa
     2821 ccgcctgttc ggccacctcg gcgatgtgct ctttcccgag ttcgagcagg tagacatagg
     2881 cgcgcagagc gatgccgaca ttgccgtaga acgaacggac gcgccccatt gagtctttga
     2941 ggagcgcaaa aacatagcgg tcgccttcct tgcgcacgac cggattaggc aggaactcga
     3001 cgaggtgctt cgccacgccc accgggcccg aaccggggcc gccgccgccg tgcggggtcg
     3061 agaaggtctt gtgcaggttg aaatgcatca catcgatccc catgtcgccg ggacgggcga
     3121 cgccgagtat cgcgttcaag ttcgcgccgt cgccgtagac cagcgcgccg gcatcgtgac
     3181 aggccttggt tatcgcgcag atatcggtct caaaaacgcc gagggtgttc gggttggtga
     3241 gcatgatcgc cgccacttca tcgttcagca tccctttcag acagtcgaga catatcttcc
     3301 cgtcggggcc cgactcgagc acgaccgcct cgaaaccgtt aatggcgcag gaagcggggt
     3361 tggtaccgtg ggccgagtcg gggataatga cctttttgcg cttggtgtcg cctctcttct
     3421 ggtgatactt gcggatgacg ccgagcccgg tgtattcgcc gtgggcgccc gccgccggat
     3481 tgagcgatac ctcggcgaag cccgacacct cggcgagcat gtgctggaga tcgaacagca
     3541 tcttgagcgt cccctgcgcg tagcccagtg gcgtctcggg gtgcaggttc gcgaatgcag
     3601 cgaggcgcgc catcttctcg ttgatcttcg ggttgtactt catcgtgcag gagccgagtg
     3661 gatagaaggt atggtcgatg ctgaagttgt aggtcgagag gcgcgtgtag tg
//
LOCUS       DAOQUE010000270         3710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_28164_length_3710_cov_3.271135, whole genome shotgun sequence.
ACCESSION   DAOQUE010000270 DAOQUE010000000
VERSION     DAOQUE010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28164_length_3710_cov_3.271135"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKH10_08590"
     CDS             <1..447
                     /locus_tag="PKH10_08590"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNT28221.1"
                     /translation="SGVPLILMHMQGTPRTMQDDPRYDDLLADINAFFDEAVARAVVA
                     GVKRERILLDPGFGFGKTAAHNLTILRHFEAFAIHRLPILAGLSRKSFIGAATGRKEP
                     ADRLAGTLAAQTLALEKGAAVIRAHDAAATRDLILMHRAVREAPCW"
     gene            438..1232
                     /gene="cdaA"
                     /locus_tag="PKH10_08595"
     CDS             438..1232
                     /gene="cdaA"
                     /locus_tag="PKH10_08595"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNT28222.1"
                     /translation="MLVTYMTAFWDSLWGGDPLVLILSLVDIALVWFFIYKFLVLIKG
                     TRSMRILAGFFLLVALYFVGKLVGMRAMVWLLGGILEYFIIIVVVIFRDEIRSILSSF
                     EFIRMLGGDRATYESMLMSEELAEALSNLAQNRDGAIVVVAQKGDPEPFVTGGVMLGA
                     EVKKELLISIFRKNTPLHDGAVVIREGRIALAGVVLPLSTNPDIDVNFGTRHRAAYGI
                     SERVDALVFVVSEERGQISLLKDGRITRNMTKEMIKRMLTKHLSQD"
     gene            1237..2160
                     /locus_tag="PKH10_08600"
     CDS             1237..2160
                     /locus_tag="PKH10_08600"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HNT28223.1"
                     /translation="MKRIRDIFTLNGREKLLALFATVAIWLGVVANREEPVNFAIKVR
                     FEAGADRVVTMTKSPEEVYVQAKGSIFAVAQVRSSDHEVVVDVSRREAGRTVYFIDDR
                     DIPFSQILKIERISPREISFLVSKKVARTVMVDPVLDGQPKKGYKIVSVESKPAMVPV
                     VGPEETIASLESVATERLDISNSTATVTKTLRVMPGQPGVTPVENDSTVIVTVVVERD
                     IREMTFSRVPFVLDGGVPSDMTPQFITVRVRGPVETLEKLAETGLNAFVENLPARLYV
                     VDRYYFKDLPADVEVVEMEQVRQITIRKKNP"
     gene            2157..3521
                     /gene="glmM"
                     /locus_tag="PKH10_08605"
     CDS             2157..3521
                     /gene="glmM"
                     /locus_tag="PKH10_08605"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNT28224.1"
                     /translation="MTRKLFGTDGVRGTANAFPVTVDNALVLGRTAVRYFQQASPIRE
                     GKHRIVVGKDTRLSSYMLEAAIAAGICSAGADAVLLGPLPTPGISFITRSMRADAGVV
                     ISASHNPYHDNGIKFFSKEGTKLTDEAELEIEEAFGQDSIEGLPIGREAGKIYRVNDA
                     QGRYVEFLKSSFPRDLALDGIEISLDCANGAAYLVAPTVLSELGATVRTYAKTPNGEN
                     INDHCGALYPENVAGKVLGDRSDVGIALDGDGDRVILVDEKGAIVDGDRIIGAAALYL
                     KEQGKLAGDLVVTTIMTNVGLELHLKEHGIAMRRTPVGDRYVFEEMQKSGAIVGGENS
                     GHIIFSEFNPTGDGILTALQILAIMQRSGRKLSELVAAIPLFPQVMKKLAVAKKIPLD
                     ELPKTRKAMADGEKALGRGRVVVRYSGTEPVLRVMAEGEDEAKCAAVVAAILDAAKEE
                     FSAG"
     gene            3553..>3710
                     /locus_tag="PKH10_08610"
     CDS             3553..>3710
                     /locus_tag="PKH10_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT28225.1"
                     /translation="MISLSSTFRALRSRNYRLYFWGQSLSLIGTWMQQVAIGWLVYGQ
                     TLDAAVIAG"
BASE COUNT          712 a         1134 c         1185 g          679 t
ORIGIN      
        1 tcgggcgtgc cgctcatcct catgcatatg caggggacgc cgcgcaccat gcaggacgat
       61 ccccggtacg acgatctgct cgccgacatc aacgcctttt tcgatgaggc ggtggcgcgg
      121 gcggtcgtgg cgggggtgaa gcgcgagcgg atactactcg accccggctt cgggttcggc
      181 aagaccgccg cgcataacct gacgatactc cggcacttcg aggcgttcgc gatacaccgc
      241 ctgccgatcc ttgccgggct gtcgcgcaaa tcgttcatcg gtgcggcgac cggccggaaa
      301 gagccggccg accggctcgc cgggacgctc gcggcccaga cgctcgcact cgagaagggg
      361 gcggcggtca tccgggccca cgacgcggcg gcgacgcgcg acctgatcct catgcaccgt
      421 gcggtacggg aggcgccatg ctggtgacct atatgaccgc attctgggat tcgctctggg
      481 gtggcgatcc gctcgtgctc atactttcgc tcgtcgacat cgcgctcgta tggttcttca
      541 tctataagtt cctcgtcctc atcaagggga cccgatcgat gcgtatactc gccgggttct
      601 tcctgctcgt cgcactctac ttcgtcggca aactggtcgg gatgcgcgcg atggtctggc
      661 tcctcggcgg catacttgaa tatttcatca tcatcgtggt ggtcatcttc cgtgacgaga
      721 tacggagcat cctgtcgagt ttcgagttca tccggatgct gggcggcgac cgcgcgacct
      781 acgagagcat gctgatgtcg gaggaactcg ccgaagcgct ctcgaatctc gcgcagaacc
      841 gtgacggcgc catcgtggtg gtcgcgcaga aaggcgatcc ggaaccgttc gtcacgggtg
      901 gcgtcatgct gggcgccgag gtcaaaaaag aactgctcat atcgatattc cgcaagaata
      961 cgccgctcca cgacggggcc gtggtcatcc gcgagggtcg catcgcgctt gccggcgtgg
     1021 tcctgccgct ctcgaccaat cccgacatcg atgtcaattt cggtacccgc catcgcgccg
     1081 cctacggcat atcggagcgt gtcgacgcgc tggtgttcgt cgtttcggag gagcgcggcc
     1141 agatatcgct cctcaaggat ggccgcatca cgcggaacat gaccaaggag atgataaaac
     1201 ggatgctcac caaacacctc tcgcaggact gacgcgatga aacggatacg cgacatattc
     1261 acgctgaacg gtcgcgagaa actgcttgct ctgttcgcaa cggtcgcgat atggctcggt
     1321 gtggtcgcga accgcgagga gccggtgaat ttcgcgatca aggtccgttt cgaagcgggg
     1381 gccgatcgcg tggtgacgat gaccaagagc cccgaggagg tctatgtgca ggcgaagggg
     1441 agcatcttcg ccgtcgcgca ggtccgatcg agcgaccatg aggtggtggt cgatgtgtca
     1501 cggcgcgagg cggggcggac cgtttatttc atcgacgacc gcgacatccc gttctcgcag
     1561 atactcaaga tagagcggat atcgccgcgc gagataagtt tcctcgtgtc caagaaggtc
     1621 gcgcgaacgg tgatggtcga cccggtgctc gacggccagc cgaagaaggg ctacaagatc
     1681 gtgtcggttg agagcaaacc cgcgatggtc ccggtggtcg gccccgaaga gaccatcgcg
     1741 tcgctggaat cggtggcgac cgaacggctc gacatatcga acagcacggc gacggtcacg
     1801 aagaccctgc gcgtcatgcc ggggcaaccg ggcgtcacgc cggtcgagaa cgacagcacc
     1861 gtgatcgtga cggtggtggt cgaacgtgac atccgcgaga tgaccttctc ccgggtgcca
     1921 ttcgtgctgg acggtggcgt gccgagcgac atgaccccgc agttcatcac ggtgcgggta
     1981 cggggaccgg tggagacgct cgagaaactt gccgagaccg ggctcaatgc ctttgtcgag
     2041 aacctgccgg cacggctgta tgtcgttgac cggtattatt tcaaagacct gcccgccgat
     2101 gtcgaggtcg tcgagatgga gcaggtgcgg cagataacca tacggaaaaa gaacccatga
     2161 cacgcaaact tttcggcacc gacggcgtgc gcggcaccgc gaacgccttc ccggtgacgg
     2221 tcgacaacgc gctcgtgctc ggtcgtaccg cggtccgtta cttccagcag gcgagtccca
     2281 tccgcgaggg gaagcaccgc atcgtggtcg gcaaggacac gcggctctcc tcttacatgc
     2341 tcgaggcggc gatagcggcc ggcatctgtt cggcgggcgc cgacgcggtc ctgctcgggc
     2401 cgctcccgac ccccggtatc agtttcatca cccgctcgat gcgggccgac gccggggtgg
     2461 tcatctcggc gagccacaat ccctaccacg acaacggcat caaattcttc tcgaaggaag
     2521 ggaccaaact gaccgacgag gcggaactcg agatcgaaga ggctttcggg caggactcga
     2581 tcgaggggct ccctatcggc cgcgaggcgg gcaagatata ccgcgtcaac gacgcgcagg
     2641 gacgctatgt cgaattcctg aagtcgagtt ttccacggga cctcgcgctc gatggcatcg
     2701 agatatcgct cgactgcgcc aatggcgccg cctatctcgt cgcccccacc gttctttcgg
     2761 aactcggcgc gacggtgcgg acctatgcca agacaccgaa cggcgagaat atcaacgatc
     2821 actgcggcgc gctgtacccc gagaatgtcg cgggaaaggt gctgggcgac cggtccgatg
     2881 tcggcatcgc gcttgatggc gatggcgatc gcgtcatcct cgtcgacgaa aagggggcca
     2941 tcgtcgacgg cgaccgcatc atcggcgctg cggcgctata cctcaaggaa caggggaaac
     3001 tggcgggtga cctcgtggtc accaccatca tgaccaatgt cggccttgaa ctgcacctga
     3061 aggagcatgg gatcgcgatg cggcgcaccc cggtcggcga ccgttacgtc ttcgaggaga
     3121 tgcagaaaag cggcgcgata gtcggcggcg agaattcggg ccatatcata ttcagcgagt
     3181 tcaaccccac cggcgacggt atcctcaccg cgcttcagat actcgccatc atgcagcgaa
     3241 gcggcagaaa actttccgaa ctcgttgccg cgataccgct ctttcctcag gtgatgaaga
     3301 aactcgccgt cgcaaagaag ataccgcttg acgaacttcc gaagacccgt aaggcgatgg
     3361 ccgacggcga gaaggcgctc ggtcgcggac gggtggtggt gcgctactcc ggcaccgaac
     3421 cggtccttcg cgtaatggcc gaaggcgagg atgaggccaa gtgcgccgcc gtggtcgccg
     3481 ccatcctcga tgccgcgaag gaagagttta gcgccgggtg atcgccgttt tatttttcac
     3541 cgtgcgggac cgatgatatc cctttcctcg acatttcgcg cgctccgttc gcgtaactac
     3601 cggctctatt tctggggtca atcgctctcg ctcatcggga cctggatgca gcaggtggcg
     3661 atcggatggc tcgtctacgg gcagactctc gacgccgccg tcatcgccgg
//
LOCUS       DAOQUE010000271         3707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_28192_length_3707_cov_4.042169, whole genome shotgun sequence.
ACCESSION   DAOQUE010000271 DAOQUE010000000
VERSION     DAOQUE010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28192_length_3707_cov_4.042169"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKH10_08615"
     CDS             complement(<1..326)
                     /locus_tag="PKH10_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28226.1"
                     /translation="MRILCFAFTLIAIAACGAKPPEPPAATNGKGGGATVALVAAAIE
                     KRERSVDITGSLLPWREATVAVEADGRIAALGADLGSTLKTGAMLVRLAPEDYEFKVR
                     QAEVEF"
     gene            complement(328..1698)
                     /locus_tag="PKH10_08620"
     CDS             complement(328..1698)
                     /locus_tag="PKH10_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT28227.1"
                     /translation="MIRYLSLALLALFAFPLAADELLTLDEALQSAVEANPTYLAAVK
                     SAEAADNKRLLVRAGFMPVISSELGYKRTTLNSAPVPGASSFQDMLGMFNPSALNPAA
                     TMPKDRSESDSYNNYSLSLLVTQNIWDFKTYYNYQGAGHTRSAVELDADAAVNNLYLT
                     VVQAYFAVLAADATLVSARGSVAQMEKRLETATAQVDNGLRTPVDKLRAEADLASARL
                     NLIKATNLRENAVKSLATAIGRDPAQGVRIDPDARSGALEIGDPAQAASDALARRPDA
                     RALKEKISAARSTAQAARGDYYPQFKANAGVSYGGYEFDALKYNWYIGAAVSWNIFAG
                     LATQTQAKDADTAVAVLELNLRALELSVRQEIESAIIAVREAREKEEPTRTMYDAAKA
                     ALELADQRFEQGLGTITDVSDAQALYEQASAALIQTRYDLETATARLKKALGLGGTAF
                     YQHKGE"
     gene            1859..2443
                     /gene="rdgB"
                     /locus_tag="PKH10_08625"
     CDS             1859..2443
                     /gene="rdgB"
                     /locus_tag="PKH10_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNT28228.1"
                     /translation="MRIVFATGNEHKLREAREIFTGVEILSILEFDKAFDPDEYGKSF
                     LENAVIKAEAARALTGGLPVLSDDSGLIVPALNGAPGIHSARWGGLNGDHVRNRAKLR
                     NDLRGVVDRRAHFLCTAVLLTADADALVATGRVDGLLIDEERGAGGFGYDPMFVPDGD
                     PRTLAEMTPDEKNALSHRGRAFRRLAAMTARHFL"
     gene            complement(2454..>3707)
                     /locus_tag="PKH10_08630"
     CDS             complement(2454..>3707)
                     /locus_tag="PKH10_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT28229.1"
                     /translation="EKRRTNSCRSEICAFAFAMFGSLSQVLPDATYTPLWPHLGLSGA
                     FFLLFVFSSWFTRFEPFLWSLLYGFFALVFLWVLFFTYANDFALSHTIQLVVVILTLD
                     LAIFVPRHLVAFALFSTVTTAAALLLSDTPGMIQALFISLIAFGHMINYISTSHKYQA
                     HRETAVSRERAEEANRMKDLFIAKTSHDLRSPLNGIIGFTQMLMADEKNPERLKMHQA
                     VLQSSEMLLRLINDLLDLSKISAGTLHIEYRPFSPRELVETIGGGCRALTASRGIDLS
                     VTVTDRVPQFLVSDRLRIGQILLNLLSNAIKFTDRGSIRVTIDLLTTTQGHPRMWLRV
                     ADTGIGIPQDQVPRIFESFVQITEDDDKRRQGLGLGLSIVKSLVDMLGGTITVESVLG
                     AGSTFSVELPVSGVEEALTETVTAP"
BASE COUNT          686 a         1074 c         1201 g          746 t
ORIGIN      
        1 ttgaattcca cctcggcctg ccgtaccttg aattcgtaat cctcgggtgc caaccggacg
       61 agcattgcgc ccgtcttgag cgtcgaaccg aggtcggcgc cgagtgcggc gatgcggccg
      121 tcggcctcga ccgcgacggt cgcttcgcgc cacggcaaaa gcgaaccggt gatgtcgacc
      181 gaccgttccc gtttctcgat cgcagcggcg accagtgcga cggtcgcgcc gcctcctttc
      241 ccgttggtcg cggcgggcgg ttcgggcggt ttcgcgccgc aggccgcgat cgcgatcagc
      301 gtgaacgcga agcagaggat acgcatatca ttctcctttg tgctgataga acgccgtgcc
      361 gccgagtccg agcgcctttt tcaggcgcgc cgtggcggtc tcgagatcgt agcgggtctg
      421 gatgagcgcg gcggaggcct gttcgtagag cgcctgcgcg tcggaaacgt cggtgatggt
      481 gccgagtccc tgttcgaacc gttggtcggc gagttccagc gccgccttgg ccgcatcgta
      541 catcgttcgg gtaggctcct ccttctcgcg cgcctccctg accgcgatga tcgcgctctc
      601 gatctcctgc cggaccgaca gttcgagcgc gcggaggttg agttccagca ccgcgaccgc
      661 cgtgtcggca tctttcgcct gcgtctgggt cgcgagtccc gcgaagatgt tccagctgac
      721 cgccgcaccg atgtaccagt tgtatttgag ggcgtcgaat tcgtagccgc cgtaactcac
      781 tccggcgttc gccttgaatt gggggtagta gtcgccgcgc gccgcctgcg ccgtggaacg
      841 cgccgctgag atcttttcct tgagagcgcg ggcatcggga cgccgcgcga gtgcgtcgga
      901 cgccgcctgt gccggatcgc ctatctccaa cgcgccggat cgcgcatcgg gatctattcg
      961 cacgccttgc gccggatcgc ggccgatggc tgtcgcgagc gatttcacgg cgttctcgcg
     1021 caggttcgtt gcttttatga gattcagccg cgccgaagca agatcggcct cggcccgcag
     1081 tttgtcgacc ggcgtccgca ggccgttgtc gacctgcgcg gtcgccgtct cgagccgttt
     1141 ttccatctgc gcgaccgagc cgcgcgccga aacgagtgtc gcgtctgcgg ccagcaccgc
     1201 gaaataggcc tgcaccaccg tcagatagag attgttgacc gccgcgtcgg cgtcgagttc
     1261 gacggcgctt ctcgtatggc cggccccctg atagttgtaa taggtcttga aatcccagat
     1321 gttctgggtg accaagagcg acagtgaata gttgttgtag gagtccgatt cggaacggtc
     1381 cttgggcatc gtggccgcgg ggttgagcgc cgacgggttg aacatcccca gcatatcctg
     1441 aaaggaggag gcaccgggca ccggggccga attgagggtg gttcgcttgt aaccaagttc
     1501 gctcgatatg accggcatga acccggcgcg gaccagcagc cgtttgttat cggcggcctc
     1561 ggcgctcttt accgcggcga gataggtcgg attggcctct acggcgcttt gcagcgcttc
     1621 gtcgagcgtc agcagttcat ccgccgcgag ggggaacgcg aagagcgcca acaacgccag
     1681 tgaaagatat cgtatcatgg ctccatctct ctccctggtc cgtcactagt ccgacgagat
     1741 cttcgagcaa agagtaaaat ttctttgccc gacatccctt tgttatgctc aatttacttg
     1801 aatttgcaat tgttttttgg aatatggggg atgtggttca cggcacgcgg aaaagaccat
     1861 gcgcatcgtt ttcgccaccg gtaatgaaca taaactgcgg gaggcgcggg agatattcac
     1921 cggcgtcgag atactctcca ttctcgagtt cgataaggcc ttcgaccccg acgagtacgg
     1981 caagagcttt ctcgagaacg ccgtcatcaa ggccgaggcg gcgcgcgcgc tcaccggggg
     2041 tctgccggta ctttcggacg attcggggct catcgttccg gcgctgaatg gcgcgcccgg
     2101 catccattcg gcccgttggg gcggcctgaa cggcgaccat gtccgtaacc gcgcgaaact
     2161 ccgtaatgac ctgcgcggcg tcgtcgatcg gcgggcgcat ttcctttgca ccgcggtcct
     2221 gctgacggca gatgccgatg cgctggtcgc taccggacgc gtggatggtc tcctcatcga
     2281 tgaggaacgg ggagccggcg gcttcggcta cgacccgatg ttcgtccccg acggcgatcc
     2341 gcggacgctc gccgagatga cccccgacga gaagaacgcc ctcagtcacc gcggtcgagc
     2401 gttccggcgt cttgccgcaa tgacggcgcg gcattttttg tgagaaggct agattagggt
     2461 gcagtgaccg tctcggtcag cgcctcttcc acaccgctca ctggcaattc caccgaaaag
     2521 gtagaacctg cgccgaggac gctttcgacc gttatcgtgc cgccgagcat atcgacgagg
     2581 cttttcacga tggaaagccc gagaccaagt ccctgtcgcc gcttgtcgtc atcttccgtg
     2641 atctgcacga aactctcgaa gatgcgcggg acctgatcct gcgggatgcc gataccggtg
     2701 tcggcgaccc gtaaccacat ccgtggatga ccttgcgtgg tcgtgagcag gtcgatagtg
     2761 acgcggatcg atccgcggtc ggtgaatttt atcgcgttgg agagcagatt aagaagtatc
     2821 tgtccgatgc gtaggcggtc ggaaacgagg aattgcggca cacggtcggt cacagtgacc
     2881 gaaaggtcga tcccgcgcga cgctgtgagc gcgcggcagc cgccgccgat cgtttcgacc
     2941 agttcgcgtg gagagaaggg acggtattcg atgtgaagtg ttccggccga tatcttggag
     3001 agatcgagca ggtcgttgat aagacgcagg agcatttccg aggactgtag caccgcctga
     3061 tgcatcttga gccgctccgg gtttttttca tcggccatca gcatctgggt gaacccgatg
     3121 atgccgttga ggggcgaacg gagatcgtgg ctcgtcttgg cgatgaagag gtccttcatc
     3181 cggttcgcct cttccgctct ctcgcgggag acggcggtct cacggtgggc ctggtatttg
     3241 tgtgaggtgc tgatatagtt tatcatgtgg ccgaacgcga tgagcgagat gaacagggcc
     3301 tgtatcatac ccggtgtatc ggaaagaagg agcgccgccg cggtcgtgac ggtcgagaag
     3361 agggcgaagg ccacgagatg acgcgggacg aatatggcca gatcgagggt caatatcacg
     3421 acgaccaatt gtatggtgtg ggagagtgcg aagtcgttcg cgtaggtgaa aaagagcacc
     3481 cacagaaaga ccagcgcgaa gaacccgtac aggagcgacc agaggaacgg ttcgaatcgg
     3541 gtgaaccagg aggagaatac aaaaaggaga aagaatgcgc ccgagagtcc gaggtgtggc
     3601 cagagcggtg tataggtcgc atcaggaagg acctgcgaaa gagagccgaa catcgcgaag
     3661 gcgaaagcgc agatctcgga cctgcaggag ttcgtgcgcc gcttctc
//
LOCUS       DAOQUE010000272         3692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_28367_length_3692_cov_3.956283, whole genome shotgun sequence.
ACCESSION   DAOQUE010000272 DAOQUE010000000
VERSION     DAOQUE010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28367_length_3692_cov_3.956283"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="PKH10_08635"
     CDS             <1..154
                     /locus_tag="PKH10_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNT28230.1"
                     /translation="ELARKAGVSVLTVDRVERGGKCRMETKRKILIALGLSLDEKSKV
                     FANEEE"
     gene            170..1222
                     /gene="pilM"
                     /locus_tag="PKH10_08640"
     CDS             170..1222
                     /gene="pilM"
                     /locus_tag="PKH10_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNT28231.1"
                     /translation="MFFSKQLLGLDMGSFYLKTAKIVKSGRRHRLKKFGFVPIPVNTV
                     VDGSIMNTYEMNDAVKTLLTKERVGDKYCAISIAGHGIISRIVSMPQLTADEFRKALK
                     GEMETHIPHDINEIYYDGIRTGVFEEGKERVILIAARQDLVGDFMQVARNAGIRPMSV
                     EIDASALANIFEVNYPEEMEGTVAILNIGASKVNVLVLSKGAISFFRDVPNGGNFITE
                     EITRRLKVSFQQAESLKSGEQTSDDSILPNQVEEVVADVAKNMVSDIQRVFDYYANIN
                     PDDQIQKIFITGGTTRSHTFVTVLRSLMQVPVEKMNPFKEIVVPPNVLDHEQMESYAH
                     IAAIGLGLALRRLDEE"
     gene            1219..1824
                     /locus_tag="PKH10_08645"
     CDS             1219..1824
                     /locus_tag="PKH10_08645"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNT28232.1"
                     /translation="MIRINLLPVGDEKYIASARIFFILSLIFIVILLGIMFANGRVLA
                     EREKESQERLDEADREIANLKQIIGKINELKEKKQKLQEKINTIIKLQEENIGPVRVL
                     DEVSLKLPSTKIWLDSMALSGTRLQFEGKSLDNQEVANFMKQLESSLFFTKVDLQRVQ
                     KDSSVNNVPVLRFSMTGDVVLAGKQAVDSAPKPAAETGQKP"
     gene            1847..2542
                     /gene="pilO"
                     /locus_tag="PKH10_08650"
     CDS             1847..2542
                     /gene="pilO"
                     /locus_tag="PKH10_08650"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNT28233.1"
                     /translation="MKNAAINKLLVIIGKQKPIYRWAGIGLVMVGLVVAYFVTTFLPH
                     QSRMEEMAEQFSQKKAEFSKMVVLVRERGQYMEEVEHLDRELKYAVTVLPDKKEIPSL
                     LKKLAEEAEKFSLDVYYFEPQGESVQGFIASVPVAIKVRGSFHEVLSFFDSVNKFARI
                     INISNITMKADPQSVQKAERAKERKKKGSGSSASSDAFSAFQPNKTIMDVSFRATTYR
                     FLSQTETSGGGAQ"
     gene            2539..3177
                     /locus_tag="PKH10_08655"
     CDS             2539..3177
                     /locus_tag="PKH10_08655"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HNT28234.1"
                     /translation="MKILSAPLLSLSALALLVGGCSSEYEIERFDYKAEYDRVSKTPV
                     PASSISVEKDITEIIKRRSTYIYSAVGKRDPFRSYFGDMKAVEQDREPVSELQRYDIS
                     DLNVTAIIWGITEPRAVVVTPERKSIIVKRGSFVGKNWGKINAILQDKIEIVEVYKDP
                     LGRKILNKLYLELPIKTVLSEGRENEQEIRDIESEESEEQKDAVKQKEKEKE"
     gene            3291..>3692
                     /locus_tag="PKH10_08660"
     CDS             3291..>3692
                     /locus_tag="PKH10_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28235.1"
                     /translation="MTVVKGGVTESFENVEDGPVIFYPAGGLLKILLPGSNIATASTL
                     AEISNGKSGARNAAMVSLGLEGGTFAVERTGKDLFLKVGDIEEIASLKMETDLRDAQV
                     SVRETDGGLFVSASETPRSVRCFMLANPDRLV"
BASE COUNT          896 a          984 c         1108 g          704 t
ORIGIN      
        1 ggaactggcg cgcaaggcgg gcgtttcggt gctgacggtc gaccgcgtcg agcgtggcgg
       61 caagtgtcgc atggagacca aacgcaagat actcatcgcg ctcggtcttt cgctggatga
      121 gaagtccaag gtgttcgcca acgaagagga gtgacgcagg gaggagatga tgttcttcag
      181 caagcaactg cttgggctcg atatgggttc attctatctc aagaccgcca agatcgtcaa
      241 aagcgggcgc cggcaccgtc tcaaaaagtt cggcttcgtg ccgataccgg tcaacaccgt
      301 ggtcgacggc agcatcatga acacctatga gatgaacgac gcggtcaaga ccctgctcac
      361 caaggagcgc gtaggggaca agtactgcgc gataagcatc gcggggcacg gcatcatcag
      421 ccgtatcgtt tcgatgccgc aactcacggc cgacgagttc cgcaaagcgc tcaagggcga
      481 gatggagacc catatccccc acgacatcaa cgagatatac tacgatggta tcaggacggg
      541 agtgttcgaa gaggggaagg agcgcgtcat cctcatcgcg gcgcggcagg acctcgtcgg
      601 cgatttcatg caggtggccc gtaacgccgg gatccgcccc atgtcggtcg agatagatgc
      661 ctcggcgctc gcgaacatct tcgaggtgaa ctatcccgag gagatggagg gaacggtcgc
      721 catcctcaac atcggcgcat cgaaggtcaa tgtgcttgtg ctttccaagg gggccatcag
      781 tttcttccgc gatgtgccga acggcggtaa cttcatcacc gaggagatca cccgtcggct
      841 gaaggtctcg ttccagcagg ccgagagtct caagtcgggc gagcagacat ccgacgactc
      901 catactcccg aaccaggtcg aagaggtcgt cgctgatgtc gcgaagaaca tggtgagcga
      961 tatccagagg gtgttcgatt attacgcgaa catcaacccc gacgatcaga tacagaagat
     1021 attcatcacc ggtggtacga cccggagcca taccttcgtg acggtgttgc gctctctgat
     1081 gcaggtaccg gtcgaaaaga tgaacccctt caaggagatc gtcgtgccgc cgaatgtgct
     1141 tgatcatgaa cagatggaat cgtacgccca tatcgccgcc atagggctcg ggttagcatt
     1201 acggaggctg gacgaagaat gatacggata aaccttcttc ccgtcggcga cgagaaatat
     1261 atcgcgtcgg cgcgcatatt cttcatcctc tcgctcatct tcatcgtcat cctgctcggc
     1321 atcatgttcg ccaacggcag ggtcctcgcg gagcgggaga aagagagtca ggaacggctc
     1381 gatgaggccg accgtgagat cgctaacctg aaacagatca tcggcaagat aaatgaactg
     1441 aaggaaaaga aacagaaatt gcaggagaag ataaacacca tcatcaaact gcaggaagag
     1501 aacatcggtc cggtccgggt actcgacgag gtgtcgctca aactgccctc gacgaagatc
     1561 tggctcgaca gcatggctct ctccggtacc cgcctccagt tcgaggggaa gagtcttgac
     1621 aatcaggagg tcgccaattt catgaaacag ctggaatcct cgctcttctt cacgaaggta
     1681 gatctgcagc gggtacagaa ggattcctca gtgaataacg tcccggtcct gaggttcagc
     1741 atgaccggcg atgtggtgct ggcgggcaaa caggcggtcg acagcgcgcc caaaccggcc
     1801 gccgaaacgg gacagaaacc ttaatcgagg aacgagggat gtgccgatga agaacgccgc
     1861 gatcaataag ctgctcgtca tcatagggaa acagaagccc atctaccgct gggccggcat
     1921 cggtcttgtt atggtgggac tcgtggtcgc ctatttcgtc accaccttcc tgccccacca
     1981 gtcgcgcatg gaggagatgg ccgagcagtt cagccagaaa aaggcggaat tctccaagat
     2041 ggtggtgctt gtccgggaaa gaggccagta catggaagag gttgagcatc ttgaccgcga
     2101 actcaagtat gcggtcacgg tcctccccga caaaaaggag ataccgtcat tgctcaagaa
     2161 actcgccgag gaggccgaga agttcagtct ggatgtctac tatttcgagc cgcaggggga
     2221 gagcgtacag ggcttcatcg cctcggtacc ggtggccatc aaggtccggg gctctttcca
     2281 cgaggtcctc agtttcttcg attcggtcaa taagttcgcg cggatcatca acatcagcaa
     2341 catcacgatg aaggcggacc cgcagagcgt ccagaaggcg gagcgggcga aggagcgcaa
     2401 gaagaaaggt tccggatcct ccgcctcctc cgatgccttt tcggcattcc agccgaacaa
     2461 gaccatcatg gatgtttcct tccgtgctac gacctataga ttcctgagtc agaccgaaac
     2521 cagcggtgga ggggcccaat gaagatactg tccgctcctt tgctgtccct gtccgctctc
     2581 gcgctcctgg tcggcggttg ttcgagcgag tacgagatag agcgcttcga ttacaaggcc
     2641 gagtacgacc gggtcagcaa gaccccggta cccgcctctt cgatcagcgt ggagaaggat
     2701 atcaccgaga tcatcaagcg ccgatccacc tacatctact cggcggtcgg caaacgggat
     2761 ccgttccgga gttacttcgg cgatatgaag gcggtcgaac aggatcgcga gccggtgagc
     2821 gaactgcaga ggtacgatat atccgatctc aacgtaacgg ctatcatctg gggcatcacc
     2881 gagccccgtg cggtggtggt gacccccgag cggaaaagca tcatcgtcaa gcgaggcagt
     2941 ttcgtcggta agaattgggg taagataaac gccatcctcc aggacaagat agagatcgtg
     3001 gaggtctata aggatccgct gggcaggaag atattgaata aactgtacct cgaactcccg
     3061 ataaaaacgg tgctcagtga aggtcgtgag aacgaacagg agatccgtga catcgaatcg
     3121 gaagagagcg aggagcagaa agatgctgta aagcagaagg aaaaagagaa agaatgagaa
     3181 ttgttcaggt ataaaaggag ctgatgatga aaagcgcgac attgacggtt tttctcgctc
     3241 ttgggctggc ctttgcgtcg gtggcctcgg caaaagcggg tggggctacc ttgaccgtgg
     3301 tcaagggcgg cgtcaccgag tcttttgaga atgtcgagga tggcccggtg atcttctatc
     3361 ctgccggcgg actactgaag atcctcctgc ccgggtcgaa tatcgccacc gcgagcaccc
     3421 tcgcggagat atcaaacggg aaaagcggcg cgcggaacgc ggcgatggtc tcgttggggc
     3481 tcgagggagg caccttcgcc gttgaaagaa caggcaagga tcttttcctc aaggtcggtg
     3541 acatagagga gatcgcttcc ctgaagatgg agaccgatct cagggatgcg caggtctccg
     3601 tccgtgagac cgatggcggg ttgttcgtct cggcaagcga aacgccccgc tcggtgcgct
     3661 gcttcatgct cgccaacccc gaccggctcg tg
//
LOCUS       DAOQUE010000273         3676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_28537_length_3676_cov_3.654791, whole genome shotgun sequence.
ACCESSION   DAOQUE010000273 DAOQUE010000000
VERSION     DAOQUE010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28537_length_3676_cov_3.654791"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PKH10_08665"
     CDS             <1..444
                     /locus_tag="PKH10_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT28236.1"
                     /translation="ITAYLPVLLEPDPTVSPDASAFTPHRGSGTVLCADDEPTIRETV
                     KAMLTIMGYEVLTAADGEEAVTLFRRYADRISFLVLDIIMPRRSGVEALREIRRDRPQ
                     LRALLISGYRFEEPTQEAVSWGGVATLAKPFTFEQFAEAIRTLTS"
     gene            523..1437
                     /locus_tag="PKH10_08670"
     CDS             523..1437
                     /locus_tag="PKH10_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28237.1"
                     /translation="MRNIILKMVSKGVISSDNANRLVNMKQDIDPLWKGIVRGYVDEK
                     KLARFFEDEGYPVIYDEGTTVFQKDDIFKFFTPDIIAKYLVFPISYKKDSRDIILGFL
                     NAAHLETVRRMIQHIFPDLSTTYFHVPYSVFRRVVYKNFLFDMSKYASMVIGMETILP
                     PSTQTDAKGSLNRNIATVAERVIIFTIENGSAVFRDEATVNRFPLASLPTIRDGLQKR
                     YLELSADHAVKKLSPTEKILLFTNMGIKRDDRMVIVSADDDSGFFILINPRISRDELI
                     KMLGELNRAGTPDETPAAAEILHLELKK"
     gene            complement(1452..1940)
                     /locus_tag="PKH10_08675"
     CDS             complement(1452..1940)
                     /locus_tag="PKH10_08675"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HNT28238.1"
                     /translation="MKKRLMSMALALTMLFSALFMQGCYGSYPLFKKVNKLVGDIDGR
                     WGSTAVHIICWVFQVYSVILFVDLFILNTVEFWTGSDPLAMNEGESETQVVTYLGDTY
                     RITATKNRFDVYCVEGSRRGESASLIWNAEGRTWVAEKEGIARNLVTIGEDGSIEGIA
                     VP"
     gene            2110..2499
                     /gene="mscL"
                     /locus_tag="PKH10_08680"
     CDS             2110..2499
                     /gene="mscL"
                     /locus_tag="PKH10_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNT28239.1"
                     /translation="MSMLNEFKEFASKGNVVDMAVGVVLGTAFGKIVTSVVNDVIMPP
                     IGLLLGGTDFTNLAITLKAAEAGAPAVMLYYGRFVQTIVDFMIVAWAIFLLVKGVNSL
                     RRLQELKIPTVPGVKIPGIAEKKKEEV"
     gene            2501..>3676
                     /locus_tag="PKH10_08685"
     CDS             2501..>3676
                     /locus_tag="PKH10_08685"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNT28240.1"
                     /translation="MTRYLTTLCLCFGLLLGAVAQAEEPVDAAKDANAAPASAKDDKK
                     GDRLFFSIGTGVNFSLTDNRDVVGQTTGTSFLIGLNLDTKLEYYKLGHEIRTNLIIDE
                     QFSYTPDLDRIIKSADKFAIDAIYLYNISADLWGPFAKAHFETQLFNGFLNTMRPEYY
                     VFPDGTTVAPFDQLQISGSFDPIKISESVGLFVKPWTREWLKTELRAGLGGLHVIADT
                     VYIAGAKDETTGRIPLTQVESFNQLGAAFAAMLSGTYKLDAPITYGASADILVPFVYD
                     DPMGRAAGELTNYNFAANVGVEVAPWLSVGYRLSVVRQPQVSEEWQIQNNLLVSFRYS
                     FEKEVPEEAAPTEAPAPATEPAPAPVAEPAPAPVAEPAPAPAAEPVSAEGSAGASPAP
                     "
BASE COUNT          789 a         1177 c         1004 g          706 t
ORIGIN      
        1 atcaccgcct acctgccggt attgctcgaa ccggacccga cggtctcccc cgacgcatcg
       61 gctttcacgc cgcaccgcgg gagcggcacg gtcctctgcg ccgatgacga accgacgata
      121 cgggaaacgg tcaaggcgat gttgacgatc atgggctacg aggtgctgac cgccgccgac
      181 ggcgaagagg cggtgacgct gttccgccgg tatgccgacc gcatctcgtt cctcgtactc
      241 gacatcatca tgccccggcg atccggcgtc gaggcgctcc gggagatacg gcgcgaccgt
      301 ccccagctcc gcgcgctgct catatccggc taccgcttcg aggaaccgac gcaggaggcg
      361 gtatcgtggg gcggcgtcgc cacgctcgca aaaccattca ccttcgaaca gttcgccgag
      421 gcgatccgga ccctgacctc ctgacggaaa atttatcttt tctttatttt ccgaccggtt
      481 ataatcgccc gaacatcgaa acgctcttcg ggagtatcgc gcatgaggaa catcatcctg
      541 aaaatggtgt cgaaaggggt catctcctct gataacgcca accggctggt caacatgaag
      601 caggacatag acccgctctg gaaaggcatc gtccggggct atgtcgatga aaagaaactt
      661 gcccgttttt tcgaggatga gggctatccg gtcatctacg acgaggggac caccgtcttc
      721 cagaaggacg acatattcaa gttcttcact cccgacatca tagccaaata cctggtcttc
      781 ccgatatcct ataaaaagga cagccgcgat atcatcctcg ggttcctcaa cgcggcccat
      841 ctcgaaacgg tgcgccggat gatacagcac atctttcccg acctgtcaac aacctacttt
      901 catgtcccct actcggtctt ccgtcgcgtc gtctacaaga acttcctgtt cgacatgagc
      961 aagtacgcct cgatggtcat cggcatggag acgatcctgc ccccgtcgac gcaaaccgat
     1021 gcgaaagggt ctctgaaccg gaacatcgcg acggtcgcgg aacgggtcat catcttcacg
     1081 atagagaacg gatcggccgt cttccgcgac gaggcgaccg tcaatcggtt ccctctcgcc
     1141 tcgctcccga cgatacgcga cggtctccag aaaaggtacc ttgaactatc ggccgatcat
     1201 gccgtgaaga aactctcgcc gaccgagaag atactcctgt tcaccaacat ggggataaaa
     1261 cgggacgacc gcatggtcat cgtcagcgcc gacgacgatt ccggcttctt catcctcatc
     1321 aacccccgca tatcgcggga cgaactgata aagatgttgg gcgaactgaa ccgggcgggg
     1381 acaccggacg aaacgccggc cgccgccgag atattacatc ttgagttgaa gaagtaggcc
     1441 gtacggcccc ctcacgggac ggcgataccc tctatcgatc catcctcgcc gatggtcacc
     1501 agattccggg caatgccctc cttctcggcg acccaggtcc gcccctcggc gttccagatg
     1561 agcgaggcgc tctcgccccg gcggctcccc tcgacgcagt agacatcgaa ccggttcttc
     1621 gtggccgtta tccggtaggt gtcgcccaga taggtcacca cctgcgtctc gctctctccc
     1681 tcgttcatcg cgagcgggtc gctcccggtc cagaactcga ccgtgttcag gatgaagaga
     1741 tcgacgaaca ggatgaccga gtagacctga aagacccagc agatgatgtg gaccgcagtc
     1801 gaaccccacc gaccgtcgat gtcgccgacc agtttgttga cctttttgaa cagcgggtaa
     1861 ctgccgtagc acccttgcat aaagagcgca gagaacagca tggtcagcgc cagagccatc
     1921 gacatgagcc gttttttcat ggggacctcc ttcaacgcgg ttaataagca ggcggaacca
     1981 gagggatggt atccgaccga aaaggaagaa tcaagatttt cataactgaa aaaaaaacta
     2041 ggggaatcct tgacttagta tctcattagc gtaccgtttg tctgtctgtc cgttttggga
     2101 ggtcaccaga tgagtatgct caacgagttc aaggaattcg catcgaaagg caatgtcgtc
     2161 gacatggcgg tcggcgtcgt gctcggcacc gccttcggca agatcgtcac ctcggtggtc
     2221 aacgatgtca tcatgccccc catcgggctg ttgctcggcg gcaccgactt caccaatctc
     2281 gccatcacgc tcaaggcggc ggaggcggga gcgccggcgg tgatgctcta ctacggccgt
     2341 ttcgttcaga ccatcgtcga tttcatgatc gtggcgtggg ccattttcct gttggtcaag
     2401 ggggtcaact ccctgcgacg acttcaggaa ctcaagatcc ctacggtccc gggagtgaag
     2461 atacccggca tagcagaaaa aaagaaagag gaggtgtgac atgacccgtt atctgacgac
     2521 cctgtgcctc tgtttcggac tcttgctggg cgccgtcgcg caggccgaag agccggttga
     2581 tgccgccaag gatgccaacg ccgcacccgc gtcggccaag gacgacaaga agggcgaccg
     2641 cctgttcttc tccatcggca ccggtgtgaa cttctcgctc accgacaatc gcgatgtggt
     2701 cggccagacg accggcacct cgttcctcat cggtctcaac ctcgacacca aactcgaata
     2761 ctacaagtta gggcacgaga tacggaccaa cctgatcatc gacgaacagt tcagttacac
     2821 acccgacctc gaccggataa taaaaagcgc cgataaattc gccatcgacg ccatttatct
     2881 ctacaacatc agcgccgatc tctggggccc gttcgcgaag gcccatttcg aaacgcaact
     2941 gttcaacggc ttcctgaaca ccatgcgccc cgagtactat gtgttccccg acgggacgac
     3001 ggtcgccccc ttcgaccaac tgcagatatc gggctccttc gatcccatca agatatcgga
     3061 atcggtcggt cttttcgtga aaccgtggac ccgcgaatgg ctcaagaccg aactccgcgc
     3121 cggcctcggc ggactgcatg tcatcgccga caccgtctac atcgccgggg cgaaggatga
     3181 gaccaccggc cggataccgc tcacgcaggt cgagagtttc aaccaactcg gcgccgcgtt
     3241 cgccgcgatg ttgtcgggca cctacaaact cgatgcaccg ataacctacg gcgcgagcgc
     3301 cgacatcctc gtgccgttcg tctatgacga cccgatgggg cgtgccgcag gggaactgac
     3361 caactacaat ttcgccgcca atgtcggggt cgaggtcgcc ccgtggctct cggtcgggta
     3421 ccgtctgtcg gtcgtgcgcc aaccgcaggt gagcgaggag tggcagatac agaacaacct
     3481 gctcgtttcc ttccgctact ccttcgagaa agaggtgccg gaagaggccg cgccgaccga
     3541 agcccccgct ccggcgactg agcctgctcc cgctccggtg gctgaaccgg cccccgctcc
     3601 tgtggctgag ccggcccccg ctccggcggc tgaacccgtc tccgccgaag gctccgccgg
     3661 ggcaagccct gccccc
//
LOCUS       DAOQUE010000274         3662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_28708_length_3662_cov_3.747436, whole genome shotgun sequence.
ACCESSION   DAOQUE010000274 DAOQUE010000000
VERSION     DAOQUE010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28708_length_3662_cov_3.747436"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKH10_08690"
     CDS             <1..217
                     /locus_tag="PKH10_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28241.1"
                     /translation="LGCCLALFYSRVRETMEGEVWFTERKFVKKAKGMAPGKVLITQG
                     KPKYLRADRSFFEKLRRDGDPLIHGDD"
     gene            217..840
                     /locus_tag="PKH10_08695"
     CDS             217..840
                     /locus_tag="PKH10_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28242.1"
                     /translation="MDHRERVKEFERFAKRFRTPHDIQRFLNETPYSSHKICLSPWRV
                     MKERHAHCAEGAYFAAAIFSLWGERPLVVDLLAENDDDHIIAPFRVDGYWGAVAKSNT
                     TVLRYREPVYKNLRELVMSYFDLYFNTDGMKSLRGYSRPVSLDRLGGKHWMTSDDDLN
                     FIGDRLTALPHIPLIDKRMRANLAPTDDDLVKACFLNSNEAGLFRPR"
     gene            complement(853..1317)
                     /locus_tag="PKH10_08700"
     CDS             complement(853..1317)
                     /locus_tag="PKH10_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28243.1"
                     /translation="MFHKFLSLAIIGALAICSVACASQKTGFSSGQKDIVVETRDPNW
                     DFSNLRKVYLYTETVNSSEERDAIAEMVKLLMPRGVECIVDTGESITYDDPSMQIRAH
                     RKLMSLLTSIYLLKPGIKGNEEIIAVISSDNPLKAAAEAIKYIEGARPATAE"
     gene            1446..>3662
                     /locus_tag="PKH10_08705"
     CDS             1446..>3662
                     /locus_tag="PKH10_08705"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNT28244.1"
                     /translation="MDKFWGFYLSLLFRYRWAVFFLFAILFVAELLYVRGNFRIDSDL
                     RALFHGENQTVKDLEYLDSRLGGYATAQIVVRSESYENNLAFLTALKKDLESFSDIRF
                     VEFERDITYLEERALLLLPTERLIEAEQKVREAVANEVEKGLSLDDQPATDRADDKMP
                     HALSDEIDHVLKEIERQKLAYNISPYYVAQGGKYVEMKVRPAGSDTTIQDIKRIVTLL
                     DERVAATGPDKYGIAVEVGGYYRHRLEEIQAINSNLVASLVACILLLLTVTVLFFRTP
                     FSVPIIFFPLSFGVMFGIIAALWVIGKFNLISAFTIAMLYGLGIDYAIHLYSRYGEFR
                     REGLPPLDAMRSTYEHLFRPLLYSTVTTAIAFLSLIFIEFKGFSDFGIVAGVGIISSL
                     VAILFLFPAIVFLIEQWTPLTHAPRGVSNIAPAYQFFLRRPIFMIIPSTILIASFIAL
                     FLVRFEYNIDNLSFKRKYSPDSIIARYNDLVRDENPNTMAASMPAIIATDSIEEMYDA
                     TRALYRKKQELNDLQHEIRIGGILSMANFVPEGQEEKLQIMKRILRVIERKKELMDDK
                     TAERFEREIRPLLSVSSPITAEDLPKWIQDKLREKDGSYGKIISFVTSGNKSDINKVL
                     TIRKELGTIHGELGEYRTRATFLLLADIKHVLEHELPRAILLSFFSVLAVLILAFRSF
                     RQAGMILTPLFAGITLMIGIIHFSGFSFNLFNMVVIPTIIGIGIDSCIHIYDRFRST"
BASE COUNT          774 a         1159 c          968 g          761 t
ORIGIN      
        1 cctcggctgt tgcctcgccc tcttttacag cagggtacgc gagacgatgg agggtgaggt
       61 ctggttcacg gaacgcaagt tcgtgaaaaa agcaaagggc atggcgcccg gcaaggtgct
      121 catcacgcag ggcaagccga aatatctgcg ggccgaccgg tcgttctttg agaaactgcg
      181 ccgcgacggc gacccgctca tacacggaga cgactgatgg accaccggga acgcgtcaaa
      241 gaattcgaac gcttcgcgaa gcggttccgc accccgcacg atattcaacg cttcctcaac
      301 gaaacgccct actcatcgca caagatatgc ctttcgccgt ggcgcgtgat gaaagagcgg
      361 catgcccact gcgccgaggg tgcctacttc gcggcggcga tcttctcgct gtggggagaa
      421 cggccgctcg tggtcgatct cctcgccgag aacgacgacg atcacatcat cgcgccgttc
      481 cgcgttgacg gctactgggg ggcggtcgcc aagtcgaata cgacggtgct gcgctaccgc
      541 gaaccggtct acaagaacct gcgcgaactg gtgatgtcct atttcgacct ctacttcaac
      601 accgacggga tgaagtcgct acgcggttat tcgcggccgg tgagtctcga ccggctgggc
      661 gggaagcact ggatgacgag cgacgatgat ctcaacttca tcggggaccg actgacggcg
      721 ctacctcata tcccgctcat cgacaagagg atgcgcgcga atctcgcacc gaccgacgac
      781 gaccttgtga aagcctgttt cctgaactcg aacgaagcgg gcctcttcag accgcgctag
      841 gacccagcaa catcactccg cggtggccgg gcgcgccccc tcgatgtact tgatcgcctc
      901 ggcggccgcc ttgagcgggt tgtcggaact gatgaccgcg atgatctcct cgttcccctt
      961 gatgcccggc ttgagcaggt agatgctggt gaggagtgac atgagtttgc ggtgcgcccg
     1021 tatctgcata ctcgggtcat cgtaggtgat gctctcgccg gtatccacga tgcactctac
     1081 gccgcgcggc atgagcagtt tcaccatctc ggcgatcgcg tcacgttcct ccgacgagtt
     1141 gaccgtttcg gtatacaggt agacctttcg caggttcgag aagtcccagt tcggatcgcg
     1201 ggtctcgacc acgatgtcct tctggcccga cgagaaaccg gtcttttgcg atgcgcaggc
     1261 gacggaacag atcgccagag cgccgatgat cgcgagcgac aggaacttgt ggaacatctt
     1321 cacctcccct cgtagttgac aacacacgaa gggagtgtat ccgccgcgaa acgcaaagtc
     1381 aaatcttgac ttgcccgttt ccccgtataa agatgtcgat accttgctaa agaggtgccg
     1441 ttcccatgga caagttctgg ggtttctatc tatcccttct tttcaggtac cgctgggctg
     1501 tttttttcct gttcgcgatc ctcttcgtcg ctgaactcct ctatgtccgc ggcaatttcc
     1561 gcatcgacag cgacctgcgt gcgctcttcc acggcgagaa ccagacggtc aaagacctcg
     1621 aatacctcga cagccgcctc ggcgggtacg ccaccgcaca gatagtcgtc cgctcggaaa
     1681 gttacgagaa caacctcgcc ttcctgaccg ccctgaagaa ggatctggag tcgttctcgg
     1741 acatacggtt cgtcgagttc gagcgcgaca tcacctacct cgaggaacgc gccctgcttc
     1801 tcctgcccac agagcggctc atcgaggcgg agcagaaggt ccgcgaggcg gtggcgaacg
     1861 aggtggaaaa aggactctcc ctcgacgatc agcccgccac cgatagggcc gacgacaaga
     1921 tgccgcacgc cctttcggac gagatagatc atgtcctcaa ggaaatagag cgacagaaac
     1981 tggcatataa catctcccct tactacgtcg cacagggcgg gaaatatgtc gagatgaagg
     2041 tgcgccccgc cggaagcgac accacgatac aggacatcaa acgcatcgtg accctgctcg
     2101 acgagcgggt cgccgccacc gggcccgaca aatacggtat cgcggtcgag gtcggcggct
     2161 attaccggca ccgattggag gagatacaag cgataaattc gaacctcgtc gcctcgctcg
     2221 tcgcctgcat actgctcctg ctgacggtga ccgtgctgtt cttccgaacg ccattctcgg
     2281 tccccatcat attcttcccg ctctccttcg gcgtgatgtt cggcatcatc gccgcactct
     2341 gggtcatcgg caagttcaac ctcatctcgg ccttcaccat cgcgatgctt tacgggctcg
     2401 gtatcgacta cgctatacac ttgtactccc gttacggcga attccggcgc gaagggctgc
     2461 caccgctcga cgcaatgcga agcacctatg aacacctgtt ccggccgctt ctctacagca
     2521 ccgtgaccac cgcgatagcc tttctttcac tcatcttcat cgagttcaag gggttttccg
     2581 atttcggcat cgtcgcgggc gtcggcatca tatcgtctct tgtcgccatc ctcttcctct
     2641 tcccggccat cgtgttcctc atcgaacaat ggacaccgct cactcacgca ccgcggggcg
     2701 tttcaaacat cgcccccgcc tatcagttct ttctccgtcg accgatattc atgatcatcc
     2761 cctcgacgat acttatcgcc tccttcatcg ccctcttcct cgtgcgattc gagtacaaca
     2821 tcgacaatct atcctttaag cgcaagtact cgcccgattc catcatcgct cgctacaacg
     2881 acctggtgcg cgacgagaac cccaacacca tggcggcgag catgccggcg atcatcgcca
     2941 ccgattcgat cgaggagatg tacgatgcga cccgggcgct ctaccgcaaa aaacaggaac
     3001 tcaacgatct gcagcacgaa atacggatcg gcggcatact gtcgatggcg aacttcgtcc
     3061 ccgaaggaca ggaggagaaa ctgcagatca tgaagcggat attgcgcgtc atcgaacgga
     3121 aaaaggaact gatggacgac aagaccgccg aacggttcga acgcgagata cgaccgcttc
     3181 tttccgtatc atccccgata accgctgaag acctcccgaa gtggatacag gataaactgc
     3241 gcgagaagga cggcagttac ggcaagatca tctcgttcgt gacctcgggc aacaaatcgg
     3301 acatcaacaa agtgctgacc atccggaagg aactgggcac catccacggc gaactcggcg
     3361 aataccgtac gcgggccacc ttcctgcttc tggccgacat caagcacgtg ctcgaacacg
     3421 aactgccccg tgcgatactc ctgtcgttct tctcggtgct cgcggtactg atactagcct
     3481 tccgttcctt ccgacaggcg ggcatgatcc tcactccgct cttcgccggc atcacgctga
     3541 tgatcggcat catccatttc tccggcttct ctttcaatct cttcaatatg gtcgtcatcc
     3601 cgacgatcat cggcatcggc atcgattcct gtatccatat ctacgatcgg tttcgctcga
     3661 cc
//
LOCUS       DAOQUE010000275         3649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_28851_length_3649_cov_4.873122, whole genome shotgun sequence.
ACCESSION   DAOQUE010000275 DAOQUE010000000
VERSION     DAOQUE010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28851_length_3649_cov_4.873122"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PKH10_08710"
     CDS             <1..361
                     /locus_tag="PKH10_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HNT28245.1"
                     /translation="TVKFQLLYVFFVISHARRRIIHFACTLNPTAEWVLQQLREAFPF
                     DEAPRYLIFDRGCPFPSLRASIRAMGISPIMTGYQSPWQNGVAERFVLSARSDLLNRV
                     IVLNEKHLHRLMKEYVA"
     gene            complement(858..1037)
                     /locus_tag="PKH10_08715"
     CDS             complement(858..1037)
                     /locus_tag="PKH10_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28246.1"
                     /translation="MEYRLLYAGQLTPVAKVDADGGILEQYVYGLGVNSPDYIVKGDR
                     KYLSSVKSLSDSDIQ"
     gene            complement(1140..1526)
                     /locus_tag="PKH10_08720"
     CDS             complement(1140..1526)
                     /locus_tag="PKH10_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28247.1"
                     /translation="MKNLNSKIVFLRLLVSLLISGILAAALYMHYQTFSSSIGASIFG
                     SIMAIAFMDYALLKNPSAMSIIASFVFSIILLFYAPSFLKGIITGGDDKDMWLSFIMI
                     IAINVFHGFLNAIIRKETVPRPPTDL"
     gene            complement(1531..2394)
                     /locus_tag="PKH10_08725"
     CDS             complement(1531..2394)
                     /locus_tag="PKH10_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNT28248.1"
                     /translation="MAKVDAAGGILEQYVYATGINSPDYIVKGDRKYRLVKDHLGSVR
                     LVVDTHDGAVVKRLDYDVSGQVINEEGDFDLIFGFAGGFRDTDTGLTKFGARWYDPET
                     ARWTSKEPLGFNGGSNFYVYAGNDPVNFVDVNGLWNDRAHQDLTASAMKDFGFDPYEI
                     LLAQTFNLKVDDDQKNNAAHYMPDNAKEAEELIEKHFQNAILCQKAGNRRSALIELGR
                     AMHTLQDKYAHGGLINNAFAWFAHAVGFLTLTEYPDTRYFNPGWTPFSNYNDAENASR
                     NLVQKFINETR"
     gene            complement(2441..3379)
                     /locus_tag="PKH10_08730"
     CDS             complement(2441..3379)
                     /locus_tag="PKH10_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT28249.1"
                     /translation="MARPLRITYPGALYHVINRGHRKDKIFRRTADREQFLARLLFVA
                     QKYRLIVHAYCLMDNHYHLLIETTKGDLPRAMHQFQSSYANYFRVKYRLVGSVFQSRY
                     KAVLVENDDYLLRLSVYIHLNPVRAGLVEEPDAYRWSSCARYLTGEKDDLVDPTRLLE
                     RGGGAENYRAILDEIGADKPETRLIYGANSVLGDEGFLGEALQRVQKKPDPRAVPEYR
                     KLREVSVDAIRKAVLAAYGVSEERLTTRGIRNDPRKAFVLLLKRHTALSLGEIGALVK
                     MKATGAGMAARQFEREVAENAGKRNRIEKLERELAR"
     gene            3542..>3649
                     /locus_tag="PKH10_08735"
     CDS             3542..>3649
                     /locus_tag="PKH10_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNT28250.1"
                     /translation="MSGHNKWSSIKHKKGAADAKRGKLFTKAIKEIQVAA"
BASE COUNT          765 a         1028 c          980 g          876 t
ORIGIN      
        1 cacggtgaag ttccaactcc tgtatgtgtt cttcgtcatc tcccatgcgc ggcggcggat
       61 catccatttc gcctgtacac tcaatcccac ggccgaatgg gtgttgcaac agctccggga
      121 ggcattcccg ttcgacgagg cgccgcgata tcttatcttc gaccggggct gtccgttccc
      181 gtcgctcagg gcatcgatcc gggcgatggg gatatcccct atcatgaccg gctatcagtc
      241 gccgtggcag aacggcgtcg cggaacgatt cgtgttgagt gcccgttcgg acttgctgaa
      301 tcgggtgatc gtgctgaacg agaaacatct gcatcggctg atgaaggagt atgtcgcatg
      361 agcggttttc ggccctcgta aacctcaccc ccaaccctct ccaaagtgga gagggagaat
      421 tcggtcacct catccaatgc gagggcgaca aagtccttct ccgttgcgga gaaggtgaag
      481 gatgaggttg tctgggcaac gaggaccgtt gtcatctggc cgtttcgcgc gactcgccta
      541 tcggcagaga gaaagagacg cggcctgaaa aggcggaact catcgctttg ccgcggctcg
      601 gtggattaca gcatcgctac acatggaaga aagcggcgta acggccgccg cttttcactt
      661 cgaagctaaa ttcggtgagg ggagaactgc atcgaaaatg gcttctgaac ggcattttct
      721 cgcggtttga cgctgtttct tcgcgtctca cctcccctac ttcgatccgc gtgagaaaaa
      781 gggtcggcaa cagggattcc caaccgtgga tcggcgcgct tttcagggtt cccgaggatt
      841 tcgaaccttt tgatcaacta ttgaatatca ctatccgaca atgatttgac agaggacagg
      901 tacttgcggt ccccctttac gatgtagtcg gggctgttca cgcccagccc atagacatac
      961 tgctcaagga tgccgccgtc ggcgtcaacc ttcgctacgg gggtgagttg tcctgcatag
     1021 agcaagcggt actccactat tgcgtccgcc tgcgggccgt agccttcggc ggaggacccg
     1081 tccttcttcg tcttggcgat gagccggttc agtttttttt cttcatcctc ttctcacgat
     1141 cacagatccg tcggcggccg agggactgtt tcttttctta ttattgcgtt gagaaaaccg
     1201 tgaaatacat tgatagcgat aatcattatg aaagaaagcc acatgtcttt gtcgtctccg
     1261 cccgtgatta ttcccttcaa aaatgacggt gcataaaaca acaatattat cgaaaaaacg
     1321 aaggatgcta ttatcgacat cgctgatgga ttttttaaca gagcatagtc cataaaagct
     1381 attgccatga tcgacccaaa gatgcttgct cctatggagg aactaaatgt ctgataatgc
     1441 atgtacaatg ccgcagccaa tatcccgctg attagaagag ataccaacaa acggagaaaa
     1501 actattttgg agtttaggtt tttcatgacc tcaccttgtt tcgttaatga acttctgtac
     1561 gagattacga gaggcgtttt ctgcatcatt ataattggaa aagggggtcc atccaggatt
     1621 gaaatatctt gtgtcagggt attcagttag ggttaggaac ccaaccgcat gagcaaacca
     1681 tgcaaaggca ttattgatta gaccgccatg cgcatatttg tcctgaagcg tatgcatggc
     1741 cctgccaagt tcgatgagcg cgcttcgtct gtttcctgcc ttttgacaaa gaatcgcgtt
     1801 ttgaaaatgc ttttctatga gttcctctgc ttccttggcg ttatcaggca tataatgagc
     1861 agcattgttc ttctgatcat catctacttt caggttaaat gtttgggcta aaagtatctc
     1921 gtatggatca aacccaaaat ctttcatagc gctggctgtc aaatcttgat gtgctcggtc
     1981 attccataac ccattcacat ccacaaagtt caccggatca ttccctgcat acacatagaa
     2041 gttgctcccc ccattgaacc cgagcggttc ctttgaggtc cagcgtgcgg tctcggggtc
     2101 gtaccaccgt gcgccgaact tcgtgagccc ggtatcggta tcgcggaagc cgcccgcgaa
     2161 gccgaagatc aaatcgaagt cgccttcctc gttgatgacc tgtccgctca catcgtagtc
     2221 gagccgcttg accaccgctc cgtcgtgggt atccaccacc agccgcacac tgccgagatg
     2281 atccttcacg agccggtact tgcggtcccc cttcacgatg tagtcggggc tgttgatgcc
     2341 ggtggcgtag acatactgct caaggatgcc gccggcggcg tcaaccttcg ccacgggggt
     2401 gaggggccca ttggggtcag ggcgcaagaa gtgagtaact tcatcgggcc agttcccgct
     2461 ccaacttctc gattcgattc cgttttcccg cattttccgc cacctccctc tcaaactgcc
     2521 gcgccgccat ccccgccccc gtcgccttca tcttcaccag cgccccgatc tcgcccagcg
     2581 acagcgccgt gtgccgcttg agcagcagaa cgaacgcttt gcgcggatcg ttgcggatgc
     2641 cccgcgtcgt caaccgctct tcgctcacac cgtatgccgc gaggaccgcc ttccgtatcg
     2701 cgtcgacgct tacttcgcgc agtttccggt attccggcac cgcgcgcggg tcgggttttt
     2761 tctgtacccg ctggagcgcc tcgccgagga aaccttcgtc ccccaacacc gagttcgcgc
     2821 cgtagatgag tcgcgtctcc ggcttgtccg ctcctatctc gtcgaggatc gcgcggtagt
     2881 tctccgcacc gccgccccgc tcgaggagcc gtgtcggatc gacgagatcg tccttttcgc
     2941 ccgtcaggta gcgggcgcaa ctgctccagc ggtaggcatc gggttcctcc accagtccgg
     3001 cgcgaaccgg gttgaggtgg atgtagaccg agagccgcag gagatagtcg tcgttctcca
     3061 cgagcaccgc tttgtagcgg ctctggaaca ccgagccgac gagccggtac ttcacgcgga
     3121 agtagttggc gtaggaactc tggaactggt gcatcgcccg cggcaggtcg cccttcgtcg
     3181 tttcaatgag caggtgatag tggttgtcca tcaggcagta ggcgtggacg atgagccggt
     3241 atttctgcgc gacgaacagc aaccgtgcga ggaactgttc gcggtcggcg gtccggcgga
     3301 atatcttgtc cttgcggtgt ccccggttga tcacatggta caatgcgccg gggtaggtta
     3361 tgcggagcgg ccgtgccatg gagggagcat agcgaacatc gcctcgcttg tcaagttata
     3421 tagttcttgc gccctgaccc ctcggtgtga gcaacgcctt ccgtgtgagc gcgaaaaatt
     3481 tgcgtaaccc gcgactttat gctagcgtcg gcccgtcaat atcttcttaa acggagggtt
     3541 catgtcaggc cataacaagt ggagcagcat caagcataag aagggtgctg ctgacgccaa
     3601 gcgtggcaaa ctgttcacca aagccatcaa ggaaattcag gtcgcagca
//
LOCUS       DAOQUE010000276         3646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_28887_length_3646_cov_4.221387, whole genome shotgun sequence.
ACCESSION   DAOQUE010000276 DAOQUE010000000
VERSION     DAOQUE010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28887_length_3646_cov_4.221387"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PKH10_08740"
     CDS             complement(<1..950)
                     /locus_tag="PKH10_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNT28251.1"
                     /translation="MGFIRGIFKFIVTMFALFGFLVFLIIAGGVAAVYQMKKDQFLIP
                     TETILALTVDGNIVEYASRDQVLREVIGPTVTMLDIVAQLENAAEDAQVKGLLLRIAD
                     HELSYAQIQEIRDAVLAFRKSGKPTVAYADSFGELTRGMASYYLATSCEEIYLQPSGT
                     LDLSGISAESPFLKGLLAKLGVQPIGETRKEYKNAWNIFTEEKYTGPHREAVHRMLET
                     IFSRMVADIAAARKIEPEKARALVDRSPFFAAEAVAERLLDGVKFRDEVLAHIEQKAA
                     CAKYTPLYRYEPTFPPDTATEATVAVVIAETRRQAEDAAEL"
     gene            complement(960..2135)
                     /locus_tag="PKH10_08745"
     CDS             complement(960..2135)
                     /locus_tag="PKH10_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNT28252.1"
                     /translation="MRLFTHEEITLRTTRFQERLRAGGVEAALLSAHSDLFYFTGSIQ
                     RGILIVPAQGAPTYFVRKDLDRARGETTAEVLPFDAAAIRDRLTSYRTIGFAADMTTV
                     AELRLWRDKLVPEGRDMPDISLPLALTKMVKTPVEIALMEKAAHINREAMRQVKELFR
                     PGMRDLDVQVALESYARRELGHQGSFWLRGSHLEAGLSLVVTGALALEPTYTDFPIGG
                     RGPSPAVAQGASGEVIGDLFVVDLIGARDGYNADSTRTFFVGRPDADLARRYEELRGF
                     LDKVVAFITPGVTGEEAWNYATALVTECDWCGWFMGLRSKVRFLGHGIGVEVNQLPVI
                     AAKQAIPFENGMTIAIEPKVFLPERGVIGIENTYLMENGRLRSLTGGTETITDITIA"
     gene            complement(2203..>3646)
                     /locus_tag="PKH10_08750"
     CDS             complement(2203..>3646)
                     /locus_tag="PKH10_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28253.1"
                     /translation="DGRIALLLVDLAGHAGGYFTQADYYPKEDYEYSNARDMIYLEIT
                     LEDMEIFDAAAHELQHLCYSNRQVLIENDWASTDLSNRWMDEGLATSAEHLYTGSHGT
                     WVFVFNSAFYNEPVGLGNGFYLWDYGNNDRVASDYALAYAFFQYLRVQADQPDGIYRA
                     IIEHADNDWHAVEAIIRDRVDPAKGLSAFMTDFRIAILVNDPDGSYGFKNEPGLTFDP
                     RRYSGTGMSLEGGGALYLPIDGSFTAPGTQGAAIQYVGVKTKAWTEEPPDTDEIPDLD
                     TDTEQPDEDIDGDVLPYGTVIAASTAFPHIYDGNGDLNQQIQDDMSGFLMQTVFSGTF
                     GASNKPIPGSGAMTTLALAVHWAAQGGEPASVTVMQQSDNGTAIINPMLELLFPTDNI
                     LPTMYLMDPTDEGSVKLILINAVGDSDSCVLAEAFSGITNVKAANNTTAASGGSITFG
                     VVNAKIYYPTETPVGDISDQFSNTTICPKE"
BASE COUNT          606 a         1083 c         1236 g          721 t
ORIGIN      
        1 agctccgccg cgtcctcggc ctggcgtctc gtttcggcga tgacgaccgc gacggtcgcc
       61 tcggtcgcgg tgtcgggcgg gaaggtcggt tcgtagcggt acagcggggt gtatttggcg
      121 caggctgcct tctgttcgat gtgggcgagc acctcgtcgc ggaacttcac gccgtcgagg
      181 aggcgttcgg cgaccgcctc tgcggcgaag aagggtgacc ggtccacgag cgcacgggcc
      241 ttttcgggct ctatcttgcg tgccgcggcg atgtcggcga ccatgcggga gaatatcgtt
      301 tcgagcatcc ggtggaccgc ctcgcggtgc ggcccggtgt acttctcctc ggtgaagatg
      361 ttccacgcgt tcttgtactc cttgcgggtc tcgccgatcg gttggacgcc gagtttcgcg
      421 aggagtccct tgaggaacgg gctttcggcc gatatgcccg acagatcgag cgttcccgag
      481 ggctgaaggt atatctcctc gcacgaggtg gcgaggtagt acgacgccat gccgcgcgtc
      541 agttcaccga acgaatcggc gtaggccacc gtcggtttgc cgctcttgcg gaaggcgagg
      601 acggcatcgc gtatctcctg tatctgggca taggagagtt cgtggtcggc gatgcggagc
      661 aggagcccct tcacctgagc gtcctcggcg gcgttctcca gttgcgccac gatgtcgagc
      721 atcgtgacgg tggggccgat cacctcgcgc agcacctgat cgcgggaggc gtactcgacg
      781 atgttgccgt ccaccgtgag ggcgagtatc gtttcagtgg ggatgaggaa ctggtccttc
      841 ttcatctgat aaacggccgc gacaccgccg gcgatgatca ggaacacgag gaacccgaac
      901 aacgcgaaca tcgtcacgat gaacttgaat atgccccgga tgaaccccat gaccgcctcc
      961 tatgcgatgg tgatatctgt tatcgtttcg gtgccgccgg tgagcgaacg caggcggccg
     1021 ttctccatga gataggtgtt ctcgataccg atgacgccgc gttcgggcag gaacaccttc
     1081 ggttctatcg cgatggtcat accgttctcg aacggtatcg cctgtttcgc cgcgatgacc
     1141 ggcaactggt taacctcgac gccgatgccg tggccgagga accggacctt cgaccggagc
     1201 cccatgaacc agccgcacca gtcgcattcg gtgacgagcg cggtggcgta attccacgcc
     1261 tcttcgccgg tgacgccggg ggtgatgaag gcgaccacct tgtcgaggaa gccgcgcagt
     1321 tcttcgtagc ggcgcgcgag gtcggcgtcg ggccggccga cgaagaaggt ccgtgtcgag
     1381 tcggcgttat agccgtcgcg cgccccgatg agatcgacga cgaacaggtc gccgatgacc
     1441 tcgcccgacg ccccctgcgc gacggcgggg gagggtccgc ggccgccgat cgggaagtcg
     1501 gtgtaggtgg gttccagcgc caaggcgccg gtgaccacga gcgacagccc cgcttcgaga
     1561 tgggagccgc ggagccagaa ggatccttga tgtcccagtt cgcgccgcgc gtaggattcc
     1621 agcgccacct gcacatcgag gtcgcgcatc cccggccgga agagttcctt cacctgccgc
     1681 atcgcctccc ggttgatgtg ggccgccttt tccatgaggg cgatctcgac gggggtcttg
     1741 accatcttgg taagcgcgag cgggagcgag atgtcgggca tatcgcgtcc ctccggcacc
     1801 agtttgtcgc gccagagccg gagttcggcg accgtcgtca tgtcggcggc gaacccgatg
     1861 gtccggtagg atgtcagccg gtcgcggatc gcggcggcat cgaacgggag cacctccgcg
     1921 gtcgtttccc cgcgggcccg gtcgagatcc ttgcggacga aataggtcgg cgctccctgc
     1981 gccgggacga tgagtatccc gcgctggatg gagccggtga agtagaacag gtcgctatgc
     2041 gccgaaagca gcgccgcctc gacgccgccg gcgcgcaacc gctcctggaa ccgcgtcgtc
     2101 cggagcgtta tctcctcgtg ggtgaacagc cgcatagttc cctccgcctg tcggaccggt
     2161 gaaagcatag cgcgttctga acggtttggc aaaaaaagag ggtcattcct tcgggcagat
     2221 cgtcgtgttg ctgaactggt cggagatatc gcccaccggc gtttcggtcg gatagtagat
     2281 cttcgcgttg accaccccga aggtgatgga gccgccgctc gccgcggtgg tgttgttggc
     2341 cgccttgaca ttggtgatgc cgctgaatgc ctcggcgagc acgcaggagt cactgtcgcc
     2401 gaccgcgttg atgaggatca gtttcacgct tccttcatcg gtcgggtcca tcaggtacat
     2461 ggtcggcagg atgttgtccg tggggaaaag gagttcgagc atcgggttga tgatggcggt
     2521 gccgttatcc gactgttgca tgaccgtgac ggaggccggt tcgcctccct gcgccgccca
     2581 atgcaccgca agagccagcg tcgtcatggc gccgcttccc ggtatcggtt tatttgaggc
     2641 gccgaaagtg ccggagaaga ccgtctgcat caggaagccg ctcatgtcgt cctgtatctg
     2701 ctggttgagg tcgccgttgc cgtcgtagat gtgggggaat gcggtactgg cggcgataac
     2761 cgtaccgtaa gggagaacat ctccgtcgat gtcctcatcg ggctgttcgg tatcggtgtc
     2821 gaggtccggt atctcgtcgg tgtcgggcgg ttcctcggtc catgccttgg tcttgacgcc
     2881 gacatactgt atcgccgccc cctgcgtccc cggcgcggtg aaggagccgt cgatggggag
     2941 atagagcgcg ccgccccctt cgaggctcat gccggtcccg ctgtaacggc gcgggtcgaa
     3001 ggtcagcccc ggctcatttt taaagccgta ggagccgtcc ggatcgttca cgaggatcgc
     3061 gatgcggaag tcggtcatga aggccgacaa ccccttcgcg gggtcgaccc ggtcgcggat
     3121 gatggcctcg accgcgtgcc agtcgttgtc ggcgtgttcg atgatcgcgc ggtagatacc
     3181 gtcgggctgg tcggcctgca cgcggaggta ctggaaaaag gcgtaggcga gcgcataatc
     3241 ggaggcgaca cggtcgttgt tgccgtaatc ccagaggtaa aaaccgttgc cgagtccgac
     3301 cggttcgttg tagaaggcgc tgttgaagac gaatacccag gtgccgtggc tcccggtgta
     3361 gagatgctcg gcggaggtcg cgagaccctc gtccatccag cggttcgaca ggtcggtgga
     3421 ggcccagtcg ttctcgatga ggacctgccg gttcgaatag cagaggtgct ggagttcgtg
     3481 ggcggccgcg tcgaatatct ccatgtcctc gagcgttatc tcgagatata tcatgtcgcg
     3541 cgcgttcgaa tactcgtaat cctccttggg atagtaatcg gcctgcgtga agtaaccgcc
     3601 cgcgtgcccg gcgaggtcga ccagcaaaag cgcgatgcgc ccgtcg
//
LOCUS       DAOQUE010000277         3637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_28977_length_3637_cov_4.065885, whole genome shotgun sequence.
ACCESSION   DAOQUE010000277 DAOQUE010000000
VERSION     DAOQUE010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28977_length_3637_cov_4.065885"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            349..1632
                     /locus_tag="PKH10_08755"
     CDS             349..1632
                     /locus_tag="PKH10_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNT28254.1"
                     /translation="MTKYLMILAALFALVACGGDESNDPVDDNALPDADALSESEIQA
                     LYQKSDGAAQRLSPALTAANTDFAFRLFRSIDAQEPGKNLFIAPFSISVALAMTYNAA
                     RNDTQDQMSHTLGYDPFSVSEVNDHFRALLDSISFVDVAAVLKTANSFWLSSGWSPRI
                     SADFIQLLKDDYDSAVGFYDDINEGCSLVNDWIAEQTEDKITDMLDPAGLDPTIVFFL
                     VNALYFKSSWTTPFEEHDTVTDTFTRRDGTTVEVPFMRMENQFSVCGNEGYSLLRLPY
                     GREKIALYIVKPSSSLSIDEQLSGLTSAIVDQCIADPLDSGLVLELPRFKLDYFVELG
                     PLLVDMGMPLLFSKSADLSGMQNGGNDLYIDFVKHRAFITVDEKGSEAGGATVVAGLP
                     KSSPLWRMSVPFLFFIRDDRSGTILFLGKVENPIE"
     gene            1744..2724
                     /locus_tag="PKH10_08760"
     CDS             1744..2724
                     /locus_tag="PKH10_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018755986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNT28255.1"
                     /translation="MKAVVYEKYGPPEVLRVAEVEKPVPKDDEVLIRIRATAATVGDC
                     NVRGFTFVPPGFKFIARLMFGLNKPKKPVIGVVFAGEVEETGRAVTLFKKGDAVYGID
                     GLKMGAYAQYKCLSEKGAVAAKPKNATFEEAAAIPFGALTAWSFLKEKVGVHAGQKVL
                     VNGASGGVGNYAVQIAKYLGAEVTGVCSTGSMDFVRSLGADHVLDHTAPGFTLGNEEF
                     DIVLNTVAAEPSFARYAGSLKPNGLYLAVAGGLKEMLQSLLPSMGGGRKVIAVFSSEK
                     KESLEALTRLVDEGKLRPTIDRVFPLDEIVAAHRHVETAHKKGCVVISVQ"
     gene            complement(2746..>3637)
                     /locus_tag="PKH10_08765"
     CDS             complement(2746..>3637)
                     /locus_tag="PKH10_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNT28256.1"
                     /translation="SVNFGDLLARKIRQVTPAQFHMPALFLMLAKLYFGIFRPRRPIL
                     GSEFAGDIDMVGSAVTRFKPGMPVFGFLGPRMGAYAEYVCLPENGILTEKPANLSYEQ
                     AAVVPYGAMMAWKMIGRLDLRPGQKVLVNGASAGIGSLAVQIAKYRGAKVTGVCGTQR
                     MAFVRSLGADEVIDYTAEDFTTRDEQWDVIFDILGKGSFSRARRVLTADGRYVYVSFK
                     SKQLFQMLWTRLTGGPRVLCASETEQLADLVTLKGLFESGVLTTVIDRTFPLEQAAEA
                     HRYVESGNKAAQVAITVTAG"
BASE COUNT          735 a         1023 c         1102 g          777 t
ORIGIN      
        1 cagttgattg ttgcgggacc catcgattaa ccgccttttc cattcttcga tgcgcccgat
       61 gtgggcatcg gcgacctccc gggcagagag ttcttttttc tccagcgcgg caatgatctc
      121 gaccgctttc atgtccagca tggcatcctc cttcgatcgg gttgacaaac atcgttcgtc
      181 gtcaaaccta ctgtaaggtc aggtcctacg caaggttacg gcgttctccc ttcgaaggaa
      241 gaaccttttt ttgccgtaca tggcgatacc ggaaagtggg ggaactgata aatttgcctc
      301 ctcttttcag tttgatataa tcgcccgttc aacttgagag ggggtcccat gaccaaatac
      361 cttatgatcc ttgcggcgct gttcgcgctg gtggcgtgcg gcggggatga aagcaacgat
      421 ccggtggatg acaacgcttt acccgacgcc gacgcgttga gtgaaagcga gatacaggcg
      481 ctctaccaga agtcggacgg cgccgcgcaa cgactgtcgc cggccctgac cgccgcgaac
      541 accgatttcg ctttccgtct gttccgctcc atcgatgcgc aagagccggg caaaaatctc
      601 ttcatcgccc ccttcagcat ttcggtggcg ctcgctatga cctataacgc ggcccggaac
      661 gatacgcagg accagatgtc ccacacgctc ggatatgatc ctttttcggt gtcggaggtc
      721 aacgaccact tccgcgctct tttggacagc atctcgttcg tcgatgtcgc cgcggtgctc
      781 aagacggcaa actccttctg gctgagttct ggatggagtc cccggatcag cgccgatttc
      841 atacaactct tgaaggatga ctacgacagc gccgtcggtt tctatgacga catcaacgag
      901 gggtgttcgc tggtgaacga ttggatcgcc gaacagaccg aggataagat caccgacatg
      961 ctcgaccccg ccgggctgga cccgaccatc gttttcttcc tcgtgaacgc cctctatttc
     1021 aaaagttcgt ggaccactcc gttcgaggag cacgataccg tgaccgatac ctttacacgg
     1081 cgcgacggga ccaccgtcga agttcccttc atgaggatgg agaaccagtt ctccgtttgc
     1141 gggaatgagg gatattcttt gcttcgtttg ccctatggcc gtgaaaagat agcgctttat
     1201 attgtgaaac cctcaagctc attgtcgatc gacgaacaac tctccggatt gaccagtgcg
     1261 atagtggatc agtgtatcgc cgaccccttg gattccggac ttgtgctgga gttgccgcgc
     1321 ttcaaactcg actatttcgt cgaactggga cccctgctcg tcgacatggg catgcctttg
     1381 ctcttttcca agtccgcgga tctcagcggt atgcagaatg gcggcaatga cctgtacata
     1441 gacttcgtca agcatcgcgc tttcatcacg gtggatgaaa agggaagcga agcgggcggt
     1501 gcgaccgttg tggcgggact tcccaaaagt tctccgctct ggaggatgag cgtacctttc
     1561 ctcttcttca tccgcgatga tcgttcgggc acgatactct tcttggggaa ggtcgagaat
     1621 ccgatcgagt aagggcggac gcggtcggaa aatgctgtta agggtccgat gggaggtttc
     1681 aatggtaacg aagaagatcg aagatgcgaa cacgcggttt ccccgaaaaa taggaggtgc
     1741 agcatgaaag cggtcgtgta tgaaaagtac ggtcccccgg aagtgcttcg ggtggcggag
     1801 gtcgaaaagc cggtcccgaa ggacgacgaa gtgttgatac ggatccgtgc gaccgccgcc
     1861 accgtgggcg actgcaatgt tcgcggattc accttcgttc cgccgggatt caaatttatc
     1921 gcccgattga tgttcggttt gaacaaaccg aagaagccgg tgatcggcgt ggtgttcgcg
     1981 ggagaggtcg aggagaccgg gcgcgcggtg acgctcttca aaaaagggga cgcggtgtac
     2041 gggatcgacg gtctgaagat gggggcctac gcgcagtaca aatgtctgtc ggaaaaagga
     2101 gccgtcgcgg caaagccgaa gaacgcgacc tttgaagagg cggcggccat cccgttcggc
     2161 gctctgaccg cgtggtcctt cctgaaagaa aaggtgggcg ttcatgccgg acagaaggtg
     2221 ctggtcaacg gcgcgtcggg cggcgtggga aattacgccg tacagatcgc caaatacctc
     2281 ggcgcggagg tgaccggggt gtgcagtacc gggagcatgg atttcgtcag atcgctcggc
     2341 gcggaccatg tgctcgatca taccgccccc ggtttcacac tggggaacga ggagttcgat
     2401 atcgttctta acaccgtggc ggctgagccc tcttttgcgc ggtatgcggg gtcgctgaag
     2461 ccgaacggct tgtatctcgc tgtcgcgggc ggtctgaaag agatgctgca gtcgctgttg
     2521 ccctcgatgg gcggcggccg gaaggtgatc gccgtctttt cgagcgagaa gaaggagtcg
     2581 ctggaagcgc tcacccggct ggtcgatgaa ggaaaattga ggccgacgat cgatagggtc
     2641 ttcccgttgg acgagatcgt ggcggcccac cggcatgtcg agaccgctca taaaaaagga
     2701 tgtgtggtga tatcggtcca gtgatatatc cgaaagcgtc ggctatcagc ccgcagtgac
     2761 cgttatcgcg acctgcgccg ccttgttgcc gctctccaca taccggtgcg cttcggccgc
     2821 ctgctcgagc gggaaggtcc ggtcgatgac ggtggtgagt acgcccgatt cgaaaagccc
     2881 cttgagcgtg acgagatccg ctaactgttc cgtttcgctg gcgcacagca ccctcgggcc
     2941 gcccgtcagc cgcgtccaga gcatttgaaa gagttgcttc gacttgaaac ttacatagac
     3001 ataccggccg tcggcggtaa gaacgcggcg cgcccggctg aacgatcctt tgccgagtat
     3061 gtcgaatatc acatcccact gttcgtcgcg cgtcgtgaag tcctcggcgg tgtagtctat
     3121 cacctcgtcg gcgccgagcg accgcacgaa cgccatccgc tgggtgccgc atacccccgt
     3181 gaccttggcc ccgcggtatt tggctatctg gaccgcgagc gagccgatcc cggcgctcgc
     3241 gccgttcacg agcaccttct gccccggccg aaggtcgagc cgcccgatca tcttccacgc
     3301 catcatcgcg ccgtagggga ccaccgccgc ctgttcgtag gataggttgg cgggcttttc
     3361 ggtcagtatc ccgttctcgg gcaggcagac atattcggca taggcgccca tccggggacc
     3421 gaggaaaccg aacaccggca tcccgggctt gaaccgcgta acggcactgc cgaccatatc
     3481 gatatcgccc gcgaattcac tgccgaggat ggggcggcgg gggcgaaata tcccgaagta
     3541 gagtttggcg agcatcagga agagggcggg catgtggaac tgtgccgggg tcacctgtct
     3601 gattttgcgg gccaacaggt cgccgaagtt gaccgat
//
LOCUS       DAOQUE010000278         3635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_29004_length_3635_cov_4.215084, whole genome shotgun sequence.
ACCESSION   DAOQUE010000278 DAOQUE010000000
VERSION     DAOQUE010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29004_length_3635_cov_4.215084"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKH10_08770"
     CDS             complement(<1..367)
                     /locus_tag="PKH10_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNT28257.1"
                     /translation="MSRAISLKGVRHHNLKGFDLEIPHEKIVVITGVSGSGKTSLAFD
                     TIYAEGQRRYVESLSSYARQFLELSEKPDVEHIEGLSPSIAIEQRGLNKSPRSIVGTT
                     TEIYDYLRLLFARAGTVHCH"
     gene            complement(386..1192)
                     /gene="zupT"
                     /locus_tag="PKH10_08775"
     CDS             complement(386..1192)
                     /gene="zupT"
                     /locus_tag="PKH10_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015338039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNT28258.1"
                     /translation="MPWENILFAFALTLFAGLATGVGSAMAFFAKKFDPRFLAVSLGF
                     SAGVMIYVSLVEIFVKAKGYLVAAHGARLGNWAAVLAFFGGIALIALIDNLVPSFENP
                     HEMREVGDAPTDATQKKALLRMGLFSALAIGIHNFPEGLATFIGGLQDPTLGISIAVA
                     IAIHNIPEGIAVSVPVYYATGSKKRAFTLSFLSGLAEPVGALIGYFLLRAFIGETMFG
                     IVFALVAGIMVYISLDELLPTAEKYGEHHLAIYGLVAGMAVMAVSLLLFL"
     gene            complement(1307..1383)
                     /locus_tag="PKH10_08780"
     tRNA            complement(1307..1383)
                     /locus_tag="PKH10_08780"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1347..1349),aa:Arg,seq:acg)
     gene            complement(1397..1490)
                     /locus_tag="PKH10_08785"
     tRNA            complement(1397..1490)
                     /locus_tag="PKH10_08785"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1452..1454),aa:Ser,seq:gct)
     gene            complement(1501..1592)
                     /locus_tag="PKH10_08790"
     tRNA            complement(1501..1592)
                     /locus_tag="PKH10_08790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Ser,seq:tga)
     gene            3494..3635
                     /locus_tag="PKH10_08795"
                     /pseudo
     CDS             3494..3635
                     /locus_tag="PKH10_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063702.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
BASE COUNT          709 a         1114 c         1151 g          661 t
ORIGIN      
        1 gatggcagtg gaccgtcccg gcgcgggcga acagcagccg caggtagtcg tagatctcgg
       61 tggtggtgcc gacgatcgag cgggggctct tgttgaggcc gcgctgttct atcgcgatgc
      121 tgggcgacag cccttcgata tgttcgacat cgggtttctc ggacagttcg aggaactgac
      181 gggcgtaact ggagagcgat tcgacatagc ggcgctggcc ttcggcgtag atggtgtcga
      241 aggcgagcga ggtcttgccc gaaccggaga cgccggtgat gaccacgatc ttttcgtgcg
      301 gtatctcgag gtcgaaccct ttgagattgt ggtgacggac ccctttgagc gatatggcgc
      361 gggacatgat aactccttaa tacctttaca ggaacaacag caaactgacc gccatcaccg
      421 ccatcccggc gacgagtccg tagatggcga ggtgatgttc tccgtatttc tcggcggtcg
      481 gcaggagttc gtcgagcgag atatagacca tgatccccgc caccagcgcg aagacgatgc
      541 cgaacatcgt ctcgccgatg aaggcgcgga gcaggaaata accgatgagt gcgccgaccg
      601 gttcggctaa acccgacaga aaggaaaggg tgaatgcccg tttcttgctg ccggtggcgt
      661 agtagaccgg caccgacacg gcgataccct cggggatgtt gtggatcgcg atggcgaccg
      721 cgatgctgat gccgagcgtg gggtcctgca ggccgccgat gaaggtcgcg agcccctcgg
      781 gaaagttgtg gatgccgatg gcgagcgccg agaagaggcc catccggagc aacgccttct
      841 tctgcgtcgc gtcggtcggg gcgtctccca cctcgcgcat ctcatgcggg ttctcgaacg
      901 acggcaccag gttatcgatg agcgcgatga gcgcgatgcc gccgaaaaag gcgagcacgg
      961 ccgcccagtt gccgagccgt gcgccgtgcg ccgcgacgag gtaacccttc gccttgacga
     1021 atatctcgac gagcgacaca tagatcatca cccccgccga aaagccgagc gaaacggcga
     1081 gaaaacgggg gtcgaacttc ttagcgaaga aggccatcgc gctcccgacg ccggtggcaa
     1141 gaccggcgaa aagggtcagc gcaaaggcga aaaggatgtt ctcccacggc atgtcgggta
     1201 cctccgagcg ctatcgtacg cggaacgggg cacttgtaaa attttcgttg cgtatcgtga
     1261 aagaacgggt ccgacccccg cagggaccgg acccggtatg aaaagatggc gcgcccgaca
     1321 tgactcgaac atgtaacctt ctgatccgta gtcagatgct ctatccaatt gagctacggg
     1381 cgcacaagtg aacaagtggc ggagagggag ggattcgaac cctcggtggg atttgagtcc
     1441 cacacccgct tagcaggcga gtaccttcgg ccaaactcgg acacctctcc atgtattgaa
     1501 tggcggagga ggtaggattc gaacccacgg aggaacttgc gtccctcaac ggttttcaag
     1561 accgcctcct tagaccactc ggacactcct ccgttgagaa agaacaaagc gttatcatat
     1621 ttgtgaaaac agcccgattg tcaacttatt ttttgctcct tttccttgat tatcggcctt
     1681 ctcgcgttag tatgacgggg acttgtgagg cggtattatg cggcgttcgt acctgatagg
     1741 catcctgaca ctggcgggga tcgcgacggc gtgctcctcc ctccagaagg cggtcgctcc
     1801 gccgacccct tcgttcagcg cggtccggaa cgaactggtc atctgcgaga cgaacgaggc
     1861 ggcggcggtc tgcgaggatc gaacgcgcga ccacctgcgt cacccgtggg gccccgccga
     1921 gcaggcggaa ctgcgctatt tcctcgcccg cttccttgca aaacagggtc gtcacgataa
     1981 ggtggtgctc ctgctccgcg atcttcccga gggtacccac tacctccgcg acggccggaa
     2041 actcggcgcc gagagcgcct ttgcggcggg cgactggaac ggcgccatcg atttttcgct
     2101 cgcggtctat ctctcgcttt ccccggaaga aaagataccg atgtcgcgca tagtgttcct
     2161 ttcctacctc taccgtggcg acaccgagaa ggcggcacgg tggtacgcca aactgaacgc
     2221 cgagaaacgg gcggcgtcgg accgcgagaa ggatgagttc ctttcccgcg atctggcggt
     2281 ccgcgaaaaa ttcgaccgtg cgttggctcg tttcgagggg cgggaagaac ccgcgactcc
     2341 cgtggccccc acagcccccg tcgccaccga ggcgcccgtc gaggcgcttc ccgagatggc
     2401 gccgcccatc gcggaggtcc cggtccccgg ggatgccgtc ctgccgtcgc tcgaccagac
     2461 gcccgactgg gggaaggtgt gcctgttcct ctccgacgag gaccggtggc gcaaggtcaa
     2521 cgaggtggtc gaggtcttct tcgactggat gctcaaggag cggccccagg atccgctgac
     2581 cccggtggcg ctccgctatc tcaccgagga ggatctcgaa cgggccttcg ccaaggcgaa
     2641 ggaggaacgc tgtttcgccg gtatcgggcc gctgttcagc gaattcgccg ccgaggatct
     2701 cgcgcgactc tcggccgcga cgccgctccc gcttctcggc ttcacgccgt ggttctccga
     2761 tacccccggt ctgctcttca acatgaaata caccaaggac cgcgaggcgg ccgacatcgt
     2821 gtcgtggctc gtctcgcgcg ggcagggacg attcgccatc gcctatcccg atgatatcgc
     2881 cggtcgccgc ctgcgtgacc tgtactggtc ggccatcgag acggcgggag gcgcggtgac
     2941 cgacgcgctc gccttcgggc cgggcgacaa ggcgctcctc gacgatgtcg aagacatgct
     3001 ctcgctgccg gagaactacc ggtcgttcct ctaccggttc agacaggaga acgccgacag
     3061 gtataagacg cccaccatga tgaaacgggc gctcgaccgc atcgagaaag tgacgccgct
     3121 gcaggccgat ctcgacacgc tgatcgtcgt cggatccccc gaacagacgg cgatccttct
     3181 gccgaccttc gcctacaaga atgtcgagtt cgcctatgtg tcgtcctcgg aaaagcaccg
     3241 catccgggag aatcagagga aactgcggga ggaactcaac atcccgtggg atatcacgac
     3301 gatacaggtg gcggccgcgt cggaggtcaa aggggacgcc gatttcgaga agaatgtcaa
     3361 caaactgctg gacgggctcg cggtcgccgc gccgtggcag gacctcggcg atgccaacgt
     3421 accgtggaaa cagttcgccg agacgttcac cgcgaagttc cagcgggcgc cctatccgat
     3481 agagcggacg ctcggcgaga tggcgacggt cctgctcgcc gcctttgcgc cgccgctggc
     3541 cgaagtcgcg ttcaagttcg gtccggccga atacttctcg ctgatggtgc tcggcctgat
     3601 cgccgccgtc gtgctcgcgc acggctcgat ctgga
//
LOCUS       DAOQUE010000279         3631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_29048_length_3631_cov_4.425056, whole genome shotgun sequence.
ACCESSION   DAOQUE010000279 DAOQUE010000000
VERSION     DAOQUE010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29048_length_3631_cov_4.425056"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /locus_tag="PKH10_08800"
     CDS             <1..1591
                     /locus_tag="PKH10_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT28259.1"
                     /translation="DIMSALVESAACPQVRITVADLTRFYRFTRTNTIAGNFHFAPIP
                     GFGDPSFLPPVDGVSDVELLERLVHFGHLDGREDLFVPDADAAQAVAMRDGAGERRDL
                     RLPGGIGSLLILPIRYGEETLGILTLVADRAGFFDDNDADFYRNIAAVIGIARAHRQA
                     KFHLRERIKELTCIYRITKLGSDLDRSLDALLREAADIIPPAFLFPEAASCRIMLDRR
                     SYTSLRYNPSKHALPVELRINGRPRGLIEVSYSTDLPTLDKGPFLSEERSLLESIAKE
                     IAVIIQRKEAEEEKNALQQQLLHADRLVTVGQLAAGMAHEINEPQGAILGFSQLIQKT
                     PDLPEQAIRDLDKIVKAAIHARDIIRKLMLFSRQMPPRKSPTDLNERVTSCLDLLEPR
                     IRKGGVRVVTELTPDIPPIVADPAQIHQILVNLVVNAVQAMDPGGTLTIRTAHDGERV
                     SLAVEDTGCGMTAETKDRIFVPFFTTKDIGEGTGLGLSVVLGIVTAHGGDIKVESEPG
                     RGSRFTVTLPLAAPEKGEDHG"
     gene            1584..1991
                     /locus_tag="PKH10_08805"
     CDS             1584..1991
                     /locus_tag="PKH10_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT28260.1"
                     /translation="MADNAERGTILIVDDSPDTLELIRRNLELARYAVLTASSVNEAK
                     SQLAQRKFDLVITDYHMPFASGIDLIRFVRDKHPGTAIIMITGYASVEGAVEAIKAGA
                     EEYLSKPFTDDELLTMVDRIFEKRRAGGDATAS"
     gene            complement(2034..3338)
                     /locus_tag="PKH10_08810"
     CDS             complement(2034..3338)
                     /locus_tag="PKH10_08810"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT28261.1"
                     /translation="MKRTLQNQLLSWRKSATRKPLILKGARQVGKSWLVREFGKVYDD
                     YVEVNFEFNRAAASLFLKDRDPIRIVRDLSLLTGKDIRPGRTLLFLDEIQECPEAITA
                     LRYFYELLPELHVIAAGSLLDFTLDAIGLPVGRVELVYLYPLSFSEFLEAMGQGRLVK
                     ALSAHDGSEPLAEAIHEKLLTLLGEYMAVGGMPAAVNQWVERRDLRECMKIHRTLIET
                     FRQDFGKYATKHQQKYIDIVFGAVPRLAGKKFVYTAVSPDVRSRELRPAFELLEKAGL
                     VHTVGNTAGNGVPLGAEVDPPYFKAIFFDVALMQSLLGLEYGSWILDPATTFVNQGAV
                     CESFAGQELLAYADPVKKGELFYWARDKRGSSAEVDYLLDVAGKVVPLEVKSGNSHIK
                     SLRLFLKEKKGSPYGIVCSTRNFSRSESIHSVPLYALSICGR"
BASE COUNT          771 a         1185 c          971 g          704 t
ORIGIN      
        1 cgacatcatg tcggccctcg tcgaatcggc cgcctgcccg caggtccgca tcacggtggc
       61 cgacctgacc cgtttctatc gtttcacccg caccaacacg atagccggga acttccattt
      121 cgcccccatc cccgggttcg gcgatccctc cttcctgccc ccggtcgacg gcgtgagcga
      181 cgtcgaactg ctcgagcggc tcgtccactt cggccacctt gacggccgtg aggacctttt
      241 cgtccccgac gccgacgccg cgcaggccgt cgccatgcgc gacggcgccg gggaacgccg
      301 cgacctccgc ctgccgggcg gcatcggctc gcttctcatc cttcccatcc gttacgggga
      361 ggagacgctc ggcatactca ccctcgtcgc cgaccgcgcc gggttcttcg acgataacga
      421 cgccgatttc taccgcaaca tagccgcggt catcggcatc gcgcgtgccc atcgacaggc
      481 aaaattccat ctccgcgaac ggataaagga actcacctgc atctatcgca tcaccaaact
      541 cggctcggac ctcgaccgct cgctcgacgc tctcctgcgc gaggcggccg acatcatacc
      601 ccccgccttc cttttccccg aggccgcctc ctgccgcatc atgctcgacc ggcgcagtta
      661 cacctcgctc cgctacaacc cgtcgaaaca cgcactcccg gtggaactgc gcataaacgg
      721 ccgaccgcgc ggactcatcg aggtgagtta ttccaccgac ctgccgacgc tcgacaaggg
      781 tcccttcctg agcgaggagc gatccctgct cgaatcgatc gccaaggaga tcgccgtcat
      841 catccagcgc aaggaggccg aggaggagaa aaatgcgctc cagcagcaac tccttcacgc
      901 cgaccggctg gtcaccgttg gtcaactggc cgccggcatg gcgcacgaga taaacgaacc
      961 gcagggcgcg atactggggt tctcccaact catccagaag acccccgacc tgcccgaaca
     1021 ggcgatacgc gatctggaca agatcgtcaa ggcggccatc cacgcccgcg acatcatccg
     1081 caaactgatg cttttcagcc gccagatgcc gccacgcaag tcgccgaccg atctcaacga
     1141 acgggtcacc tcctgcctcg acctccttga accgcgcata cggaagggcg gcgtgcgcgt
     1201 cgtgaccgaa ctgacccccg acatcccgcc gatagtcgcc gacccggccc agatacacca
     1261 gatcctcgtg aacctcgtcg tgaacgcggt gcaggcgatg gaccccggcg gcacgctgac
     1321 catccggacc gcgcacgacg gggagcgggt gtcgctcgcc gtcgaggaca ccggctgcgg
     1381 catgaccgcg gagaccaagg atcggatctt cgtccccttc ttcaccacca aggacatcgg
     1441 agaaggaacg gggctcggcc tgtcggtcgt gctcggcatc gtcaccgcgc acggcggcga
     1501 catcaaagtt gaaagcgagc cgggacgcgg cagtcgtttc accgtcaccc tgccgttagc
     1561 ggcacccgaa aaaggagaag accatggctg acaacgcaga acggggcacg atcctcatcg
     1621 tggacgattc gcccgacacg ctggaactca tccggcgcaa ccttgaactg gcccgctacg
     1681 ccgtcctgac cgcttcgagc gtgaacgagg cgaagagcca gctcgcacag cggaagttcg
     1741 atctcgtcat caccgactat cacatgccct tcgcgagcgg catcgatctc atccgcttcg
     1801 tgcgcgacaa acatcccggc acggcgatca tcatgatcac cggctatgca tcggtcgaag
     1861 gggcggtcga ggcgatcaag gcgggcgccg aggagtacct ctcgaagccc ttcaccgacg
     1921 acgaactgct gacgatggtg gaccggatat tcgagaaacg ccgcgcgggc ggcgacgcaa
     1981 cggcttcatg agatcgatgg aaccgtgaga ggcatcatcc cggcttctga tgactatcga
     2041 ccgcagatac tcaatgcata caagggaact gagtggatgc tctccgaacg actgaagtta
     2101 cgggtcgaac aaacgatacc gtagggagat cctttctttt ccttcagaaa gagacgtaga
     2161 ctttttatat gactgttgcc gctcttgacc tccagcggga cgacctttcc tgccacatcc
     2221 aacagatagt cgacctcggc gctgcttccc cgtttatcac gcgcccagta aaacagttcc
     2281 cctttcttga cgggatcggc atacgcgagc agttcctgtc ccgcgaaact ctcgcacacc
     2341 gccccctgat tgacgaaggt tgttgcgggg tcgaggatcc acgaaccgta ttcgagtccg
     2401 agtagcgact gcatgagcgc cacatcgaaa aagatcgcct tgaaatacgg gggatcgacc
     2461 tccgccccta acggaacgcc gttccccgca gtgttcccga ccgtatgcac caatcccgcc
     2521 ttttccagaa gttcaaaggc gggccgcagt tcacgggaac ggacatccgg cgaaacggcg
     2581 gtatatacga atttcttgcc cgccagtctc ggcaccgcgc cgaacacgat atcgatatat
     2641 ttctgctgat gtttcgttgc gtatttgccg aaatcctgcc ggaaggtctc tatgagcgtt
     2701 cgatgtatct tcatgcattc cctgagatcg cgccgctcta cccattgatt gaccgccgcc
     2761 ggcatgccac ccaccgccat atactcgccg agaagcgtaa gaagtttttc atggatggcc
     2821 tccgctaacg gttcgctccc gtcatgcgcg gaaagggctt tcaccagacg accctgtccc
     2881 atcgcttcga ggaattcgga aaaagagagc gggtaaagat ataccagctc tacccggccc
     2941 accggcaatc cgatcgcatc aagggtgaaa tcaaggagcg aaccggcggc gatgacatga
     3001 agctccggca gtagttcata gaagtacctc agcgcggtga tggcttcggg acattcctgt
     3061 atctcgtcaa ggaacagaag cgtgcgtccc ggtcggatat cttttccggt cagaagggaa
     3121 aggtcgcgga cgatgcggat ggggtcgcgg tctttcaaga acagagaggc ggccgcgcgg
     3181 ttgaattcga aattcacctc gacatagtcg tcatatacct tgccgaactc gcggaccagc
     3241 caactcttcc cgacctgtcg cgcccctttg agtataagcg gcttacgggt cgccgacttt
     3301 ctccatgaaa gaagttgatt ttgcagtgtg cgtttcatgt tatgccctcc gttcacacta
     3361 ccaatgtctc gatttttggc tgaaagtcaa ggattaattt tattatttat ggccacaaat
     3421 cggggataaa aagtctttcc catggttaat tatcacgcta tcgcctgctt gttctcttcc
     3481 gaaaaaatct attggctttc attttccgtt cagctatact cgtgacatgg ctgggagaca
     3541 acacatcatc atcgttgctt ttgcggtttt tcttgccgcc tgcaatctac acaccgcaaa
     3601 gaatgacgcc gatacggtga ccgcggacga c
//
LOCUS       DAOQUE010000280         3630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_29062_length_3630_cov_5.291748, whole genome shotgun sequence.
ACCESSION   DAOQUE010000280 DAOQUE010000000
VERSION     DAOQUE010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29062_length_3630_cov_5.291748"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PKH10_08815"
     CDS             <1..872
                     /locus_tag="PKH10_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28262.1"
                     /translation="DDGNDLTENDACFEGDCNGDPIPGICGNAIPVVALPYTTTGDLT
                     GRPSGLTTYYGPLCTGESEPTSDIVYELTVEAGVEYRIAVMLGESDLVALNLLGVCGE
                     SEACLTYGASGPGGGVEMTYAPEAGGTVYIAIEGSGAYELSIAVVEQPDDDSVEPDDI
                     LPDDIATDDILPDETLLDEEVVDEEEPDEVVIDDVMTDDVPIDEEESDVDSVTDEEVT
                     DDTVTDADAVKPDIAPDKDSTVTDTTVTDTTVVDNEVPDETGDELLGDEDAFIPDDNT
                     KPKDDGCSCSLIP"
     gene            984..3302
                     /locus_tag="PKH10_08820"
     CDS             984..3302
                     /locus_tag="PKH10_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNT28263.1"
                     /translation="MCVFALFSPLAILAGDSFASVEGYPHATPLFPNYIQFEQTLAVQ
                     NGRIGSVLYPAPGAPALVTEEEDELSVFAACGTDTVDRVDIGKDSDSGFVAFLTLFPR
                     GQLPVGNGVTRFFFDVPYRLPHGVYDLNVTIGGATIRVDNGVGFIPEERIAFTVWTDP
                     QIEDLQSKSDINMNWNDHSYPGFSDDLLDYSRQQGIIAIAISQMSAMDTDFIACLGDL
                     VFGINHQREYEDIVQYLEKAEIPFFGVPGNHDGYAKFQSEEDFASPLERDGLNYWRAF
                     IGPTYFSFKIFGKTLMMLNTYDGTAERRASGKPIGIGDNAAPPVSNYGGFLSDPQLLW
                     ATESFAVGEVAMLFSHHLPLGMVNDGYEYTAMEKYPKNKILGALFGEEWNIETVAYDS
                     NILDTIFAESGTYNTGIRLVGAMLTEIPRPPMYFAGHTHGDALYHYEAGDEVIPGTGL
                     FTPQDLYLTQTKSAASSGQTYWGIRPVYQDLDTDTTSISYICVDDGSCAPSLDGENAG
                     FPSIPLGNFWVEYTAGETTSLYIGGNGTAETAQAVVVNFLPTDETYRLRFVMPAKELG
                     YLFDAADPIIDDAMLSADRKSILISAKGTVAAGSTLEGFLKREFIRNDASATLSPDTV
                     AAPEPEVEYEEWIPWADPLHFTVKNGDDYLHLIWYRDGKPVRDGAEFKEKFQPYHTKE
                     AIELLYIAKNGAYGVKQFAVDVEEEPETDDDLITDEDELIKDEKDESDISDDNFMADT
                     DSPLTTHDSLLPDDDALTAKPKDEGCGCSVVF"
     gene            3383..>3630
                     /locus_tag="PKH10_08825"
     CDS             3383..>3630
                     /locus_tag="PKH10_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28264.1"
                     /translation="MRNIILTVVALVGILCTGVLSAADGDERWVAMNPGSSGTNGSVL
                     AMAQHGEYLYVGGDFTLIGNVLANYVARFHIPTGTWEPL"
BASE COUNT          771 a         1104 c         1059 g          696 t
ORIGIN      
        1 gcgatgacgg caatgacctg accgagaacg atgcctgctt tgagggtgac tgtaacggcg
       61 acccgatacc gggcatctgc ggcaacgcga taccggtggt cgctctaccc tacacgacga
      121 ccggtgacct gacggggcgc ccgagcgggc tcacgaccta ttacggtccg ctctgcacgg
      181 gagagagcga gccgaccagc gacatcgtgt acgaactgac ggtggaggca ggtgtggagt
      241 accggatcgc cgtgatgctg ggcgagagcg acctggtcgc gctgaacctg ctaggcgtgt
      301 gcggagaaag cgaggcgtgt ctcacctacg gggcatccgg tcccggcggc ggggtggaga
      361 tgacctatgc ccccgaagcg ggcggcacag tgtacatcgc aatagaggga agcggagcat
      421 acgaactgtc gatcgcggtg gtggaacagc ccgacgatga ctcggtagag cccgacgaca
      481 tcctgcccga tgatatagcg accgatgata tccttcccga cgaaaccttg ttggacgagg
      541 aggtcgttga cgaggaggag ccggacgaag tggtcatcga cgatgtgatg acggacgatg
      601 ttccgataga cgaagaagag agcgatgttg acagcgtgac cgatgaagag gtcacggatg
      661 atacggtcac cgacgctgat gcggtaaagc cggatatcgc ccccgataag gatagcaccg
      721 ttaccgacac cacggtcacc gataccaccg tcgtggacaa cgaggtcccc gacgaaacgg
      781 gcgatgaact gctcggcgat gaggatgcct tcatccccga tgacaacacc aagccgaagg
      841 acgacggttg ttcctgttcc cttattccgt aaacgacccg ttctgaactg acacgccggt
      901 ctatttttct tgcctttctc cgtgcaattg ttatcctttc gtgaattaaa aatggggcga
      961 cgatgacttt acggttagcc gctgtgtgcg ttttcgcact tttttccccg ctcgcgatac
     1021 tcgccggcga ctcgttcgcc tcggtcgagg gctatcctca cgcgacgccg ctgttcccga
     1081 actacatcca gttcgagcag acgctcgcgg tgcagaacgg tcgcatcggt tcggtgctct
     1141 atcccgcccc cggcgccccg gcgctcgtga ccgaggagga ggacgaactt tcggtgttcg
     1201 ccgcctgcgg taccgacacg gtggaccgcg tcgatatcgg caaggatagc gatagcggtt
     1261 ttgtcgcctt cctgaccctc ttcccgcgcg gccaactgcc ggtcggcaac ggcgttacgc
     1321 gcttcttctt tgatgtgcct taccggttgc cgcacggggt gtacgatctc aatgtcacca
     1381 tcggcggggc gaccatccgg gtcgataacg gcgtcgggtt catccccgag gaacggatcg
     1441 ccttcaccgt ctggaccgac ccgcagatag aggacctgca gtcgaaaagc gacatcaata
     1501 tgaactggaa cgatcactcc tatcccggtt tctccgacga cctactggac tattcacgcc
     1561 agcaggggat catcgcgatc gccatatccc agatgagcgc catggacacc gatttcatcg
     1621 cctgcctcgg cgatctggtg ttcggcatca accaccagcg cgaatacgag gatatcgttc
     1681 agtacctcga aaaagccgag atacccttct tcggcgtgcc gggcaatcac gacggttacg
     1741 cgaagttcca gagcgaggag gatttcgcct cgccgctgga gcgcgacgga ctcaactact
     1801 ggcgcgcctt catcggcccc acctactttt ccttcaagat attcggcaag acgctgatga
     1861 tgcttaacac ctatgacggt accgccgagc gccgcgcctc gggcaagccg atcggcatcg
     1921 gcgacaacgc cgcgccgccg gtcagcaact acggcggctt cctctccgat ccacagttgc
     1981 tgtgggcgac cgaatcgttc gccgtcggcg aggtggcgat gctcttctcg catcatttgc
     2041 cgctcgggat ggtcaacgac ggctacgagt acaccgcgat ggagaaatat ccgaagaaca
     2101 agatactcgg cgcccttttc ggcgaagagt ggaacatcga aacggtagca tacgacagca
     2161 atatactcga cacgatcttc gccgagagcg gcacttacaa caccggcata cggctcgtag
     2221 gcgcgatgct gaccgaaatc ccccgtccgc cgatgtactt cgcgggccat acccacggcg
     2281 acgccctcta ccattacgag gcgggcgacg aggtcatccc cggtaccgga cttttcacgc
     2341 cgcaggatct ctacctcacc cagaccaaga gcgccgcaag ttcgggtcag acctactggg
     2401 gcattcgtcc cgtctatcag gacctcgata ccgacacgac gagcatctcc tatatctgcg
     2461 tcgatgacgg ctcctgcgca ccctccctcg atggtgagaa tgcgggattc ccttcgatac
     2521 cgctcggcaa cttctgggtc gagtacactg cgggcgaaac gacctcgctc tacatcggcg
     2581 gcaacgggac ggccgaaacg gcgcaggcgg tcgtcgtgaa ctttctgccg accgacgaaa
     2641 cctaccggct ccggttcgta atgccggcga aggaactcgg ctacctcttc gacgcggccg
     2701 atccgatcat cgacgatgcg atgctctcgg ccgaccgcaa gagcatcctg atctccgcga
     2761 aggggaccgt ggcggccggc tcgacccttg aagggttcct gaaacgggaa ttcatccgga
     2821 acgatgcgtc ggcgaccctc tcacccgata ccgtcgccgc ccccgagccg gaggtcgagt
     2881 acgaagaatg gataccgtgg gccgatccgc ttcacttcac cgtgaagaac ggggacgatt
     2941 atctccacct catctggtac cgcgacggca aaccggtccg cgacggcgcc gaattcaagg
     3001 agaagttcca gccctaccat acaaaagagg cgatagaact gctctacatc gccaagaacg
     3061 gcgcgtacgg cgtcaagcag ttcgcggtcg atgtcgagga agaaccggag accgacgacg
     3121 acctcatcac cgacgaggat gaattgataa aggacgaaaa ggacgaaagc gacataagcg
     3181 acgacaactt catggccgac accgattcgc cgctcacgac tcacgactca cttctccccg
     3241 acgacgacgc cctcaccgcc aaaccgaagg acgaaggctg cggctgttcg gtggtgttct
     3301 agcgccatct ttccatttga caatcatcac ggtttctgat taaatccaac agaatatctt
     3361 tcattgaacc gggggcgggt ccatgcggaa cataattctt acggtggttg cgttggttgg
     3421 cattctctgt accggtgttt tatcggcggc cgacggcgac gagcgctggg tcgcgatgaa
     3481 tccgggatcg tccggtacga atggtagcgt tcttgccatg gcgcagcacg gggagtatct
     3541 gtatgtcggc ggcgatttta ctttgatcgg caatgtcttg gctaattatg tggctcgctt
     3601 ccatatcccg accggcacat gggaaccgct
//
LOCUS       DAOQUE010000281         3629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_29076_length_3629_cov_3.889480, whole genome shotgun sequence.
ACCESSION   DAOQUE010000281 DAOQUE010000000
VERSION     DAOQUE010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29076_length_3629_cov_3.889480"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PKH10_08830"
     CDS             complement(<1..569)
                     /locus_tag="PKH10_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28265.1"
                     /translation="MRHRSTIFLIALLLSGTLLVWQACAAPEEDDPGTDTCDPANGDA
                     DCNYVEYCASVNVLDENGYPTGVEKYECILRDTCSYDMQDCAIDWYCSEDGYCFKGTA
                     PQPDNNQQPDDTTDTTLPDTAIPDTAQPDEDTAMPDTVQPDTDTAQPDETVPDTDTTG
                     VTLDFSEDFESGSDKWTLEGIWQVGTPSDG"
     gene            720..1313
                     /gene="def"
                     /locus_tag="PKH10_08835"
     CDS             720..1313
                     /gene="def"
                     /locus_tag="PKH10_08835"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNT28266.1"
                     /translation="MTLRRIAVAGSAVLKKRAVEIPEITPEVRQLAADMTETLQAAGG
                     LAIAAPQVGEPTRLVVVDRGYLNWDALPDEERKDRGASVFGPETLVNPEITSREGEDE
                     IEEGCLSVPGYRAPVKRARRITYRWTDLDGAEHERNAEGLEAICIQHELDHLDGKLFI
                     DRISRVKRTIALGKVRKYIKELDPEEDETAYRLYGES"
     gene            1279..2202
                     /gene="fmt"
                     /locus_tag="PKH10_08840"
     CDS             1279..2202
                     /gene="fmt"
                     /locus_tag="PKH10_08840"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNT28267.1"
                     /translation="MRPRIAYMGSPECAVPFLDLAHRKGADLIVFTRADKVRCRGGKL
                     SQTPVKKRALELGLSVHDLSMKSDEAHRILSDFSPDLYLVVAYGQILSERILDLPKIA
                     PINVHFSLLPKYRGAIPVQAAILAGDDTTGTTIMVMDKALDTGDIVWQETLTILPDET
                     AEHLFGRLITLSVDLLDRTWDDLVSGRFARIPQAGEASYTKLIKKDDLVVDWTRQALE
                     LHRMIRAFAYKPGVRAHFRDKLLLLGAAQWRPDITGAPGEIVAVEKKGFAVACGEGGL
                     FLTEVQPECKACMPAASFVAGYRPRIGEKLC"
     gene            2196..3506
                     /locus_tag="PKH10_08845"
     CDS             2196..3506
                     /locus_tag="PKH10_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNT28268.1"
                     /translation="MLIGERAQNPKTAAAALTRKVLERSLFVNELLNNTLDTFPLAER
                     RFITELTYGTIRYLVHLDFWIEQASGRAISRIDREVLSHLRIALYQMLFMDNREAPQI
                     VHEAVDIVKGSSRREAAGFTNFTLREILRIGPSRSKMLRLLGYDHDRFYRIWHSFPGW
                     LADLIGDLVGDRHRYKYLGFANKPLGITLRIEGGAARRDAVIAELAAQGVSAEPAAES
                     PYGIYTDRAVTWRMIAEHTDIHIQDESSQLAVLDMDLKVGDRVLDLCAAPGGKTIFAS
                     WLVGPEGRVTAADVNPYKLKNIAEALLRAGRRNVEIRLQDGAEHNPAWDAAFDAVLLD
                     APCSALGTVRRHPEVKWLKKPTDGAQMARLADRILDNAARYVRVEGTLLYSVCTFTRE
                     ETTEQMQKFIVKHPDFKVEKAYYTVSTLLDNRDVFFIARLRRMR"
BASE COUNT          702 a         1174 c         1089 g          664 t
ORIGIN      
        1 ccgtccgacg gagtgccgac ctgccagatc ccttcgagcg tccacttgtc cgacccgctc
       61 tcgaaatcct ccgaaaaatc gagcgtgaca ccggtcgtgt cggtgtcggg gaccgtttcg
      121 tcgggttgcg cggtatcggt atcgggctgt acggtgtcgg gcatcgccgt atcctcatcg
      181 ggctgggcgg tatcggggat cgccgtgtcg ggcagggtgg tgtcggtcgt atcatcgggt
      241 tgctggttgt tgtcgggttg cggcgccgtc cccttgaagc agtacccgtc ctcggagcag
      301 taccaatcga tggcgcagtc ctgcatatcg taggagcagg tatcgcgcag gatgcactcg
      361 tacttctcaa cgccggtcgg gtacccgttc tcgtccaaca cattgactga ggcacagtac
      421 tccacatagt tgcagtcggc gtcgccgttg gccgggtcac aggtgtcggt gccgggatca
      481 tcctcctcgg gcgccgcaca ggcctgccac accagcagag tgccgctcaa aagcagggca
      541 atcaggaaga tcgtcgaacg gtgtctcatg ctctttccct cagatcggtc gttcacgcgg
      601 ccggatggta ccacattgga cggctttttc aacaaaaaac tccttttttg ccctcgtgcc
      661 gctttacatc ggtctttttt taccttatac tggcgagcgc tttttatcac ggaggttcca
      721 tgaccctgcg caggatagcg gtcgccggct cggcggtcct caaaaaacgg gcggtcgaga
      781 taccggagat aacgcccgaa gtgcgacaac tcgccgccga catgaccgag accctgcagg
      841 cggcgggcgg gctcgccatc gcggcgccgc aggtcggcga gccgacgcga ctcgtcgtcg
      901 tggaccgcgg ctatctgaac tgggatgcgc ttcccgatga ggaacggaaa gaccgcggag
      961 cctcagtttt cggccccgaa acgctcgtca accccgagat aacatcacgc gagggcgagg
     1021 atgagatcga ggaggggtgc ctgtcggtgc cagggtaccg cgcgccggtg aaacgggccc
     1081 gtcgtatcac ctaccgctgg accgaccttg atggcgcgga acacgagcgg aacgccgagg
     1141 ggctcgaggc gatctgcatc cagcacgaac tcgaccacct cgacggcaaa ctcttcatag
     1201 accgcatctc ccgggtgaaa cggaccatcg ccctcggcaa ggtaaggaaa tacatcaaag
     1261 aactcgatcc ggaggaggat gagaccgcgt atcgcctata tggggagtcc tgagtgcgcc
     1321 gtcccctttc ttgatctcgc ccatcgcaaa ggcgccgacc tcatcgtgtt cacccgtgcc
     1381 gacaaggtgc ggtgccgcgg cgggaaactt tcacagaccc cggtcaaaaa gcgggcgctc
     1441 gaactcggtc tgtcggtgca cgacctatcc atgaaaagcg acgaagcgca ccgcatcctg
     1501 agcgacttca gccccgacct ctatctggtc gtcgcctatg ggcagatact atctgaacgg
     1561 atcctcgatc tgccgaagat cgcgccgatc aatgtccatt tctcgctcct ccccaagtac
     1621 cgcggcgcga taccggtgca ggcggctatc cttgccggcg acgatacgac cggcaccacg
     1681 atcatggtga tggataaagc gctcgacacc ggcgacatcg tctggcagga gacgctcacc
     1741 atactacccg acgagacggc tgagcacctg ttcgggcgac tcatcacgct gtcggtcgat
     1801 ctgctcgacc gcacctggga cgacctcgtc tcgggccgct tcgcccgcat cccgcaggcc
     1861 ggcgaggcaa gctatacgaa attgataaaa aaagatgatc tcgtcgtcga ctggacgagg
     1921 caggccctcg aattgcaccg catgatacgg gcctttgcct acaagcccgg cgttcgggcg
     1981 cactttcgcg acaaactcct gctcctcggc gcggcgcagt ggcgtcccga tatcaccggc
     2041 gcaccgggag agatcgtcgc ggtcgagaaa aagggctttg cggtcgcgtg cggcgaaggg
     2101 ggcctgttcc tcaccgaggt ccagcccgaa tgcaaagcat gcatgccggc cgcttccttc
     2161 gtcgcggggt atcgcccgcg gatcggggag aaactatgct gataggcgaa cgcgcacaga
     2221 accccaagac ggcagccgcg gcactgaccc gcaaggtgct cgagcggtcg ctgttcgtca
     2281 acgaactgct caataacacc ctcgacacct ttccgctcgc cgaacgccgt ttcataaccg
     2341 aactgaccta tggcacgata cgctatctcg ttcatctcga tttctggata gaacaggcgt
     2401 cgggcagagc catctcgcgt atcgaccggg aggtgctctc tcacctgcgc atagcgctct
     2461 atcagatgct tttcatggat aaccgcgaag cgccgcagat cgtgcacgag gcggtcgata
     2521 tcgtgaaggg ctcgtcgcgt cgcgaggcgg cgggctttac caacttcacc ctgcgcgaga
     2581 tactgcgcat cggcccgtcg cgatcgaaaa tgctccgcct gctcgggtat gaccacgacc
     2641 gcttctaccg catctggcat tcgttcccgg gctggctcgc cgatctcatc ggcgacctcg
     2701 tcggcgaccg gcaccgatac aaatacctcg gcttcgcgaa caagccgctc ggcatcacct
     2761 tacgcatcga gggcggcgcg gctcgacgcg acgcggtcat cgccgaactt gcggcgcagg
     2821 gcgtgagcgc cgaaccggcg gccgagagtc cctacggcat ctacaccgac cgcgcagtga
     2881 cctggcgcat gatcgccgag cacaccgaca ttcacataca ggacgagtcg agccaactcg
     2941 ccgtcctcga tatggatctc aaggtcggcg accgcgtgct cgatctctgc gccgcgccgg
     3001 gcggcaagac gatcttcgcc tcctggctcg tcggccccga gggacgcgtg accgccgccg
     3061 atgtcaaccc gtacaaactc aagaacatcg ccgaggcgct cctgcgggcg gggcggcgca
     3121 atgtcgagat acgcctgcag gacggcgcgg agcacaaccc cgcatgggat gccgcgttcg
     3181 acgcggtgct tctcgacgcc ccctgctcgg cgctcggcac cgtacggcgt cacccggagg
     3241 tgaagtggct caagaaaccg accgatggcg cccagatggc gcgactcgcc gatcggatac
     3301 tcgataacgc cgcccgctat gtacgcgtgg aaggaacgct cctctattcg gtctgcacct
     3361 tcacccgtga ggaaacgacc gaacagatgc agaaattcat cgtcaagcac cccgacttca
     3421 aggtggaaaa ggcctactac accgtctcga cgctcctcga caaccgcgat gtgttcttca
     3481 ttgcgcggct gagaaggatg cggtgaactc gcccctgccc ctctcttggc aagagagggg
     3541 tggcggtgca ccggggtgag ttagtacggc cgttcgaacg actctttcgt gaacttctct
     3601 tcgaccacgc gccagtaact cgcgcgctt
//
LOCUS       DAOQUE010000282         3614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_29250_length_3614_cov_4.131498, whole genome shotgun sequence.
ACCESSION   DAOQUE010000282 DAOQUE010000000
VERSION     DAOQUE010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29250_length_3614_cov_4.131498"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1245)
                     /locus_tag="PKH10_08850"
     CDS             complement(40..1245)
                     /locus_tag="PKH10_08850"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005366383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNT28269.1"
                     /translation="MKVFVVNCGSSSIKYQLFDMTDESVVGKGIAEKIGLPMGIVSLK
                     IEGTGEKVERELPLPNHEVALATILELLTVKNIIRDKAEIGIIGHRLVHAGEFYHSSV
                     VVTEDVLEKMSECNDLAPLHNPHNITGVRTAQKLFPNAFQGGCFDTAYHQTMPDYAYM
                     YPIDHSWYEKYKVRRYGFHGTSHGYVAQQAAEFLKKPLAKLRVITAHLGNGSSIAAIK
                     NGESVDTSMGMTPLEGLMMGTRCGDIDPALVFFAARKGVSLDDFDKLLNKRSGMMGVG
                     GISNDMRDNWKAIHEGNKRAELAMKMFTYRLKKYIGAYAAAMGGVEAIIFTGGIGEND
                     WEVRQMACEGLEYMGVELDPDKNKGLRSKLADISKASSKVKMLVVPTNEELVIARESY
                     RLYNETKKR"
     gene            1407..2039
                     /locus_tag="PKH10_08855"
     CDS             1407..2039
                     /locus_tag="PKH10_08855"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNT28270.1"
                     /translation="MTERKPKIVVARHTPFLVTGLTDFFDGKGNRLEVKPVMALCRCD
                     ESKMFPYCDGAHAEKGLDCCKLPERPKDKMRDYVGKQITVHFNLGVCSHHGYCLELNS
                     VFDLKKRPWIQPDNGTVEEIIATIERCPSGALAYTIDGVYHDRLERPPAIKVTKRGPF
                     EITGGIELETDDGSKPQCDEHYCLCRCNKTKNQPFCDGSHLPKKLIGSEN"
     gene            complement(2043..>3614)
                     /locus_tag="PKH10_08860"
     CDS             complement(2043..>3614)
                     /locus_tag="PKH10_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28271.1"
                     /translation="ELDMPALAESNYFNVFWNYGQKIVAAAKKVRATDDMFAIYLSNF
                     NCGPDSFLLSYVEEEMKGKPMLILELDEHDSDGGYRTRVEAFMDVVKSFRKKGGLRRA
                     GKPTMPDVFTASRPVDPKKGIVWIPPMHESVSRMFAAAFRSAGFASQAIPTENEEDLM
                     LGKRSTRGCECLPMTLTIGGMLKKAAQENHGTRHILFMPTAEGPCRFGQYNLLERKVF
                     WNEGHENIEILAPSSVNSYQGIEDKTRRAIVHGIYAGDILMKMTTKVRPYEMTSGDTD
                     ELFEKSLRKLEDGLEKGTDLRDILPGIVDDFVRVPRRHEKRPLVGIVGEIYVRCNRFA
                     NGEVIRVIERNGGEAWLSPIHEWVSYTMYLQDYMAKQQGFSLFGTGESLMKNLYFLQT
                     EKSYYKLADRLLADRHEPHMKDVLEEGKKYLPIDFSGEAILTVGRTILFARQGARMVV
                     NAAPFGCMPGTITSSLFLELKDQLNMPIVSQFYDGDLDINDKVSAILKTIVQNEEDGR
                     DIEDDDEQPGSAAAG"
BASE COUNT          679 a         1110 c         1055 g          770 t
ORIGIN      
        1 gcaatgatct ttttgcgcgt catactcccc tccgtagcac tatctcttct tcgtttcgtt
       61 atagagccgg tacgattcgc gggcgatgac caactcctcg ttcgtcggca cgacgagcat
      121 cttcaccttc gatgacgcct ttgaaatgtc ggcgagtttc gagcggagcc ccttgttctt
      181 atcgggatca agttcgacgc ccatgtattc gagcccttcg caggccatct gccgaacctc
      241 ccaatcgttc tcgccgatac cgccggtgaa gatgatcgcc tcgacgccgc ccatcgccgc
      301 cgcgtaggcg ccgatgtact tcttgagccg gtaggtgaac atcttcatgg cgagttcggc
      361 ccgcttgttc ccctcgtgga tcgctttcca gttgtcgcgc atgtcgttgg atatgccgcc
      421 gacccccatc atgcccgacc tcttgttgag cagtttgtcg aagtcgtcga gcgaaacgcc
      481 cttgcgcgcc gcgaagaata cgagcgccgg gtcgatgtcg ccacagcggg tccccatcat
      541 gagcccttcg agcggcgtca tccccataga ggtgtcgacc gactcgccgt tcttgatggc
      601 cgctatcgaa ctgccgttgc cgaggtgggc ggtgatgacg cgcagtttcg cgagcggctt
      661 tttaaggaac tcggccgcct gctgcgccac ataaccgtgc gaggtgccat ggaagccgta
      721 gcgacgcacc ttgtatttct cgtaccacga atggtctatc ggatacatgt aggcgtaatc
      781 gggcatggtc tggtgatagg ctgtgtcgaa acaaccgccc tgaaaggcgt tcgggaacag
      841 tttctgggcc gtccggacgc cggtgatatt gtgcgggtta tggagcggcg cgagatcgtt
      901 gcattcggac attttctcga gtacatcctc ggtgacgacc accgacgaat ggtaaaattc
      961 gccggcatgg acgagccggt ggccgatgat gcctatctcc gccttgtcgc ggatgatgtt
     1021 cttgacggtc agcagttcga gtatcgtggc gagggcgacc tcgtggttcg ggagcggcaa
     1081 ttcgcgctcc actttttcgc cggttccctc tatcttgagc gatacgatgc ccatcggcag
     1141 accgatcttc tcggcgatac ccttgccgac gacgctctcg tcggtcatgt cgaagagttg
     1201 gtacttgatc gaggaacttc cgcagttgac aacgaagact ttcatgggcg acctccttgc
     1261 gcggtttgaa tgtcaaagca cttcaccata acggccgcct tcccgtttgg caagaaaaat
     1321 agggggacca cctcaacggg gccgaaaact tgcctatcgg ggcggtcggc gttacatttt
     1381 ccggcaaccg aaggagggaa caactcatga ccgaacggaa gcccaagatc gtcgtcgcgc
     1441 gccacacccc gttcctcgtg acggggctca ccgacttttt cgacggaaaa gggaaccggc
     1501 ttgaggtgaa gccggtcatg gcgctctgcc gctgtgacga atcgaagatg ttcccctact
     1561 gcgacggggc gcacgccgaa aaggggctcg actgctgcaa actgcccgaa cgacccaagg
     1621 acaagatgcg cgactatgtc ggcaaacaga tcacggtcca cttcaatctc ggcgtctgtt
     1681 cgcaccatgg ctactgtctc gaactcaact cggtgttcga cctcaagaag cggccatgga
     1741 tacagcccga caacgggacg gtcgaggaga tcatcgccac gatagaacgc tgtccgagcg
     1801 gggcactcgc ctacactatc gacggggtct atcacgaccg gctggagcgg ccaccggcga
     1861 taaaggtcac caaacgcggg ccgttcgaga taacgggcgg catagaactg gagaccgacg
     1921 acggatcgaa gccgcagtgc gacgaacatt attgcctgtg ccggtgcaac aaaacgaaga
     1981 atcagccctt ctgcgacggc tcgcacctgc cgaaaaaact gataggatcg gagaactgac
     2041 cgttacccgg cggcggcgct cccgggctgt tcgtcgtcat cctcgatatc gcgcccgtcc
     2101 tcttcgttct gcacgatggt cttgagtatc gccgacacct tgtcgttgat gtcgagatcg
     2161 ccgtcgtaga actgcgacac gatgggcatg ttgagttggt ccttgagttc gaggaagagc
     2221 gaactggtga tggtccccgg catgcacccg aacggcgcgg cgttcaccac catccgcgcc
     2281 ccctgccgcg cgaacagtat cgtccgcccg acggtgagta tcgcctcgcc cgagaaatct
     2341 atcggcaggt atttcttccc ctcctcgagc acatccttca tgtgcggttc gtgccggtcg
     2401 gcgagcaggc ggtcggccag tttgtagtaa ctcttctcgg tctggaggaa gtagaggttc
     2461 ttcatgagcg attcgccggt gccgaacagc gagaagccct gctgtttcgc catgtagtcc
     2521 tgcaggtaca tcgtgtagga tacccattcg tggatgggcg acagccacgc ctcgccgccg
     2581 ttgcgctcga tgacgcggat cacctcgccg ttggcgaagc ggttgcaccg cacatagatc
     2641 tcgccgacga tgccgacgag cggccgcttc tcgtggcggc gcggcacccg cacgaaatcg
     2701 tccacgatgc cgggcaggat gtcgcgcagg tcggtccctt tctcaagccc gtcctcaagt
     2761 ttgcgcaaac tcttttcgaa cagttcgtcg gtgtcgcccg aggtcatctc gtaggggcgc
     2821 accttggtcg tcatcttcat aaggatgtcg ccggcgtaga tgccgtggac gatggcacgc
     2881 cgcgtcttgt cctcgatgcc ctgataactg ttgaccgagg agggcgccag tatctcgatg
     2941 ttctcatgcc cctcgttcca gaacaccttg cgctcaagca ggttgtactg gccgaaccta
     3001 cacggtccct cggcggtcgg catgaacagg atgtggcgcg tcccgtggtt ctcctgcgcc
     3061 gccttcttga gcatcccgcc gatcgtgagc gtcatcggca ggcattcaca gccacgggtg
     3121 gaccgtttgc cgagcatgag gtcctcctcg ttctcggtcg gtatcgcctg cgaagcgaac
     3181 ccggccgacc ggaacgcggc ggcgaacatg cgcgacaccg actcgtgcat cggcggtatc
     3241 cagacgatcc ctttcttggg gtcgaccggg cgcgacgcgg tgaacacatc gggcatcgtc
     3301 ggcttgccgg cgcgcctgag accgcccttc ttgcggaaac ttttcaccac atccatgaac
     3361 gcctcgacgc gggtccggta gccgccgtcg gaatcgtgct catcgagctc cagtatcagc
     3421 atcggcttcc ccttcatctc ctcctcgacg taactcagga ggaacgaatc ggggccgcag
     3481 ttgaagtttg acagatagat cgcgaacata tcgtcggtcg cgcgcacctt cttggccgcc
     3541 gccactatct tctggccgta gttccagaac acattgaagt agttgctctc ggcgagcgcc
     3601 ggcatatcga gttc
//
LOCUS       DAOQUE010000283         3600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_29406_length_3600_cov_5.927504, whole genome shotgun sequence.
ACCESSION   DAOQUE010000283 DAOQUE010000000
VERSION     DAOQUE010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29406_length_3600_cov_5.927504"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PKH10_08865"
     CDS             complement(<1..616)
                     /locus_tag="PKH10_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNT28272.1"
                     /translation="MTRFHEYLSEHFETIFRWFTILCAGTVLLIMAGMFINLAWNARL
                     AIGEFGLGFLWSDAWDPALNEYGAFSSIYGTLVSTLIAMAIAVPMSVVIALFLVELAH
                     PTVSKVVGSAIELLAAIPSIIYGMWGLFVFAPIMAETVQPFLQETLGLEKVPGLGRLF
                     TGAPMGIGMLTAGLILALMILPFISAVMRDVFAMVPPVVKESAYG"
     gene            823..1851
                     /gene="pstS"
                     /locus_tag="PKH10_08870"
     CDS             823..1851
                     /gene="pstS"
                     /locus_tag="PKH10_08870"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HNT28273.1"
                     /translation="MKKLIAALSALIAFAALSAAETINGAGASFPFPLYSQWAYDHNK
                     ITGVQLNYQSIGSGGGIAQIKAKTVDFGASDEPLKEEELNGAGLFQFPMIIGGVVPVV
                     NLKGIAEGKLQLSGETLAQIYLGKIAKWNDPKIAAENPGLALPDQKIVVVHRSDGSGT
                     TWIFTNYLTKASPDWASAVGNAKAVKWPTGVGGKGNEGVAAFVKQTGGSIGYVEYAYA
                     LKNKMAYAKLKNKAGKFVTPSIETFQSAAANADWKNAKGFYMVLTDQPGDASWPIVGA
                     SYILVYKAMPDAAKAQTMLKFFDWCFKNGEKTAKSLDYVPLPKNLVEMIQTKWKSDLK
                     VASQPVWK"
     gene            1929..2210
                     /locus_tag="PKH10_08875"
     CDS             1929..2210
                     /locus_tag="PKH10_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNT28274.1"
                     /translation="MFKVNEYFNGTVKSIGFMTNEGRATVGVMAPGEYEFGTDTIEHM
                     TVVAGELVVQLPDETAWKSFGPGHTFTVDKGKKFKLKVKVDTAYLCRYV"
     gene            complement(2211..2906)
                     /locus_tag="PKH10_08880"
     CDS             complement(2211..2906)
                     /locus_tag="PKH10_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT28275.1"
                     /translation="MARERILVVDDEQDILELIRYNLSREGYQIDAVTSGEEAIKIAR
                     DRHPDLIILDLMLPGIDGLDVCRLLKGETRTAHIPIIMLTAKGEDSDVVTGLELGAED
                     YMTKPFSPKVLIARVRNILRRKTRTAEEKEVKMVIIEDMKIDPSKHKVTIGKEDIDLT
                     ATEFALLMFLAKRPGWVFTRSQIINGVKGDDYPVTDRSVDVQILNLRKKLGDMGEYIE
                     TVRGVGYRFKELA"
     gene            complement(2997..>3600)
                     /locus_tag="PKH10_08885"
     CDS             complement(2997..>3600)
                     /locus_tag="PKH10_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28276.1"
                     /translation="DDPMMAVVQIGAAIKVSDFDATATLSYYDPMNLMNAETNTGEGT
                     KMLGYIKQGYYWKNIAAALDASYTIEEKYQIGFLGEMTANIYDLNKDALGAKADDNLA
                     FFVGLKGGTKALKDLGDFQSSISYRQLGISSWIPFLIDADPLDGNVNAEGIKFDIKAG
                     LYKNFSAAATLNYFWTLDKKFNSDKTNKDLILQLDLNYKF"
BASE COUNT          745 a         1036 c         1077 g          742 t
ORIGIN      
        1 tgccgtaggc cgactccttg accaccggcg gcaccatcgc gaagacatcg cgcatcaccg
       61 cgctgatgaa gggaagtatc atgagggcga gaatgagccc cgcggtaagc ataccgatgc
      121 ccatcggcgc gccggtgaac aggcgaccga ggccggggac cttttcgagt ccgagcgttt
      181 cctgcaggaa cggctggacc gtttcggcca tgatcggcgc gaagacgaag agcccccaca
      241 tcccgtagat gatggagggt atcgccgcga gcagttctat cgccgagccg accaccttcg
      301 agaccgtggg atgggcgagt tcgacgagga acagggcgat gacaacactc atcgggaccg
      361 ctatcgccat cgcgatgagc gtcgatacga gcgttccgta aatgctcgaa aaggcgccgt
      421 actcgttaag cgccgggtcc catgcgtcgc tccagaggaa cccgagcccg aactcaccga
      481 tggcgagccg ggcgttccac gcgagattga tgaacatccc cgccatgatg agaagcaccg
      541 tgccggcgca gaggatggtg aaccacctga atatcgtttc gaaatgttcc gacaggtatt
      601 cgtgaaagcg ggtcatgcgt tccctcgaaa aggtacggtc cgaaggtagc cgccgattgt
      661 taactaacgg ttagcggacc gttaattgcc cgacaattcc cgttccgttc gtggtattct
      721 catctttcct taatcgggaa ttaacagagg catcgtaata ctttcatcgt ccggcgccgg
      781 tgcgccggag ggtttttgaa actaccaaga ggagaggatc ttatgaagaa gttgatcgcg
      841 gcgctcagcg cgcttatcgc cttcgccgcc ctttcggcgg ccgagaccat caatggcgcc
      901 ggggccagtt tcccgttccc gctctattcg cagtgggcct acgaccacaa caagatcacc
      961 ggcgtgcaac tcaactatca atcgatcggt tcgggcggcg gcatcgccca gatcaaggcc
     1021 aagacggtcg atttcggtgc ctccgacgaa ccgctcaagg aggaggaact gaacggcgcg
     1081 gggctcttcc agttcccgat gatcatcggc ggggtggtcc cggtggtgaa cctcaagggg
     1141 atcgccgagg gcaaactgca gttatcgggc gaaacgctcg cgcagatata cctcgggaag
     1201 atcgccaagt ggaacgaccc gaagatagcg gccgagaatc cgggactcgc tcttcccgac
     1261 cagaagatcg tcgtggtcca ccgctcagac ggctccggca ccacgtggat attcaccaac
     1321 tacctgacca aggcgagccc cgattgggcc tcggcggtcg gcaacgccaa ggcggtgaaa
     1381 tggccgaccg gtgtgggcgg caagggcaac gaaggggtag cggcgttcgt caagcagacc
     1441 ggcgggtcga tcggctatgt cgaatacgcc tacgccctca agaacaagat ggcctacgcc
     1501 aaactcaaga acaaggcggg caagttcgtc accccctcga tcgagacctt ccagagcgcg
     1561 gcggccaacg ccgactggaa gaacgcgaag ggtttctaca tggtcctcac cgaccagccg
     1621 ggcgacgcca gttggccgat agtcggcgcc tcctacatcc tcgtttataa agcgatgccc
     1681 gatgccgcaa aggcgcagac gatgctcaaa ttcttcgact ggtgcttcaa gaacggcgaa
     1741 aagaccgcca agtcactcga ctatgtgccg ctcccgaaga acctcgtcga gatgatacag
     1801 accaaatgga agagtgatct caaggtggcc agccagccgg tgtggaagta agttccctcc
     1861 cttttctttc ttgacaatct tcccatcatc ggcgaaccta tcctcgccac tttatgaggg
     1921 agcatgcgat gttcaaggtc aatgagtatt tcaacggcac ggtgaagtcc atcggtttca
     1981 tgaccaacga aggcagggcc acggtcggcg tcatggcgcc gggcgagtat gaattcggca
     2041 ccgacaccat tgaacacatg acggtggtgg cgggcgaact ggtcgttcag ttaccggacg
     2101 agacggcgtg gaagagcttc ggccccggcc acaccttcac cgtcgacaaa ggcaagaagt
     2161 tcaaactgaa agtgaaggtc gataccgcct atctctgccg gtatgtctga tcaggcaagt
     2221 tccttgaacc ggtagccgac gccgcgcacc gtctcgatgt attcccccat atcgcccagt
     2281 ttcttgcgca ggttgagtat ctgcacatcg accgagcggt cggtcaccgg gtagtcgtcg
     2341 cccttgacgc cgttgatgat ctggctgcgg gtgaagaccc agccgggccg cttggcgagg
     2401 aacatgagga gcgcgaattc ggtcgcggtg aggtcgatat cctccttgcc gatggtgacc
     2461 ttatgcttcg agggatctat cttcatatcc tcgatgatca ccatcttgac ctctttctcc
     2521 tcggccgtcc gcgtcttgcg ccgcaggatg ttgcggaccc gcgcgatgag caccttgggg
     2581 ctgaagggct tggtcatgta atcctcggcg ccgagttcga gcccggtcac cacatcgctg
     2641 tcctcccctt tcgccgtcag catgatgatg ggaatgtggg cggtgcgcgt ctcgcctttg
     2701 agcaggcgac agacatccag cccgtcgatc ccggggagca tgaggtcgag gatgatgagg
     2761 tcgggatggc ggtcgcgggc gatcttgatc gcctcctcgc cgctggtcac cgcgtctatc
     2821 tgataccctt cgcgcgacag gttgtagcgg atgagttcga ggatatcctg ttcatcatcg
     2881 accaccagta tccgttcgcg tgccatggtc acctcccctt ggttgacgct ccgtcggcac
     2941 agtatagagg aagtgttagg agaatgccag tccccggtta attttcggtt aggagatcag
     3001 aacttgtagt tgaggtcgag ttggaggatg aggtccttgt tggtcttatc tgagttgaat
     3061 ttcttgtcga gcgtccagaa atagttcagc gtcgcggcgg ccgagaaatt cttgtaaagt
     3121 cccgccttga tatcgaactt gataccttcg gcgttcacat tgccgtcgag cggatcggcg
     3181 tcgatgagga acggtatcca cgacgagatg ccgagttgcc ggtaggatat cgaactctgg
     3241 aaatcgccga ggtctttgag cgccttggtg ccgcccttga gcccgacgaa gaaggcgagg
     3301 ttgtcgtcgg ctttcgcccc cagcgcatcc ttattgagat cgtagatgtt cgccgtcatc
     3361 tcgccgagga atccgatctg atatttctcc tcgatggtgt aagatgcgtc gagcgccgcg
     3421 gcgatattct tccagtagta gccctgcttg atgtagccga gcatcttcgt cccttcgccg
     3481 gtgttcgttt cggcgttcat caggttcatc gggtcatagt aggagagggt cgcggtcgcg
     3541 tcgaagtcgg acaccttgat cgccgcgccg atctgcacca ccgccatcat cgggtcgtcg
//
LOCUS       DAOQUE010000284         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_29511_length_3591_cov_2.797794, whole genome shotgun sequence.
ACCESSION   DAOQUE010000284 DAOQUE010000000
VERSION     DAOQUE010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29511_length_3591_cov_2.797794"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PKH10_08890"
     CDS             <1..911
                     /locus_tag="PKH10_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28277.1"
                     /translation="QDWDPSSWIFFSFMLFYSMIFGDAGYALTIIAALLYARHRLPNP
                     APGIRRFLNLSLALSGGAVVYGIITGGFYGLSSQNPLFGWVVRLALIDTDISHPGVLS
                     KMMTISIVIGLIHITISILLKAGRAIFSLKQPIAAVPLLAWLPGIWGFYFWASLKDSD
                     PATAAICLKIFIAAIIAVFLTSAGTWRPFKFLFGGFLGVYNGVQFFADVLSYLRLFAL
                     GLSGSLIAQNFNMLAMMVWDAGPLYFTILPAALIFVAGHALNIALCVMGGVIHGLRLN
                     FLEWYRWSFDGGGRPFKAFRNLLADR"
     gene            994..3240
                     /locus_tag="PKH10_08895"
     CDS             994..3240
                     /locus_tag="PKH10_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT28278.1"
                     /translation="MSNLWKALYGLTEKHPYIVLTILSAALFIPLLGSYPLLGQWETH
                     YGRVAMEMLLNKNGIWDWFLDPIYLDQYNFWSKPIFCFWMVLPFYAVLGPTEWATRLP
                     FAINGILCLLLVYWTIKRFFHGDHSRAFVTALVLMLTPFYYLITKQFMWDITAVTFIY
                     GGVMFLFLGMRDDDRRLVRLAYVFWGLAMLAKGLLAVVIPGGIFFLWMLATADYGLLA
                     EKGGVKKVFQQYGDFLKKARILEGIGLFLIVSAWWYLYMYAKHGLPFLKEFFVENHFG
                     RMEGLIDKPDGPFEFYLWQIAIGAFPWFALFIPGLIFAGDHKEHKREEGFIILSFFFV
                     FLFFTLSATKFPHYIFPAMPFFAAIVAIPIIEFFRGERRTLYPLAAVLGALILGVTGK
                     DLGTGINYRELLYIITTHRIQDWFGRVYDMLPWLYALVPPMVFFLLLPLVRLKSLLLM
                     RISLVGFSLVAVAFASYINFYWVPKVLWVFTPKQLVEKYLEVKEPGDLIVDYDNWKNR
                     CLYFYLGPDEQLIRANTPAAVKKLIEDNPTHAVYITTKKNKVPELRSVLMASGVPLTK
                     IMDDAVDTYMEVEMYRASMKEKGSSDDSWKKDLITEDKIPNNIQRTGSTLDDKTVEII
                     GYKLNKNYFEQGEKIVIDMYYRVLKTTEKNWQIFFHLDVFEGALPHSIKIDEYPLKGF
                     YPTDKWQVGDIIHDQFEATVARGHPGGGIKIYTGFYIGNDRMKVDEQKFNDGVNRFIL
                     GTFNIKVK"
     gene            3245..>3591
                     /locus_tag="PKH10_08900"
     CDS             3245..>3591
                     /locus_tag="PKH10_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28279.1"
                     /translation="MRLLSFLLFTAIAVSCGDNGRGTPLNTDDLPAEEDLFDLSDGSD
                     LPDLSDLPDQTDQSEPEEQDDLMELPDEDEGPDLTVNDIDHSLFGQAGACGTVPATPG
                     VTTDACGFIVNGA"
BASE COUNT          673 a         1173 c          947 g          798 t
ORIGIN      
        1 atcaggactg ggacccgagt tcgtggatat tcttctcatt catgctgttc tactcgatga
       61 tcttcggcga cgccgggtac gcattgacca tcatcgcggc gctcctgtac gcgagacacc
      121 ggttgccgaa ccccgctccg ggcatccggc ggttcctcaa cctttcgctc gccctttcgg
      181 ggggcgcggt ggtctacggc attatcacgg gcggtttcta cggcctttcg agccagaatc
      241 cgttgttcgg ctgggtggtg cggctggccc tcatagatac cgatatctcc caccccgggg
      301 tgctttccaa gatgatgacc atatccatcg tcatcggact catccacatc accatctcaa
      361 tcctcctcaa ggcgggacgc gccatctttt cgctgaaaca accgatcgcg gccgtcccgc
      421 tcctcgcgtg gctccccggc atctggggtt tctatttctg ggccagcctc aaggacagcg
      481 acccggcaac ggccgccatc tgcctgaaga tattcatcgc cgcgatcatc gccgtcttcc
      541 tgacgagcgc cgggacctgg cgcccgttta aattcctgtt cggcggcttc ctcggcgtct
      601 acaacggcgt ccagttcttc gctgatgtcc tgagttacct ccgtttgttc gcgctcggcc
      661 tttcgggctc cctcatcgcg cagaatttca atatgctcgc gatgatggtc tgggacgccg
      721 gcccactcta cttcacgata ctcccggcgg ccctcatctt cgtcgccggg catgccctca
      781 atatcgcgct ctgcgtcatg ggcggcgtca ttcacggcct tcggctcaat ttccttgaat
      841 ggtaccgctg gtccttcgac ggcggcggac ggccgttcaa ggcgttccgg aaccttttgg
      901 ctgaccggtg aaccttcccc ccgataaggt tgatttttct cctccgactg gtatgctgac
      961 ccgcgattga taaggtacct gtgggagact gccatgtcga acctctggaa ggcgctctac
     1021 ggcctgaccg agaaacatcc ctatatcgtg ctgacgatcc tgtcggccgc cctgttcatc
     1081 ccgctgctgg gaagttatcc gcttctcggt cagtgggaga cgcactatgg tcgcgtcgcg
     1141 atggagatgc tgctcaacaa gaacggcatc tgggattggt tcctcgatcc catttacctt
     1201 gaccagtaca atttctggag caagccgata ttctgtttct ggatggtgct ccccttctac
     1261 gcggtgctcg gaccgaccga gtgggcgacc cgcctgccct tcgccatcaa cggcatcctc
     1321 tgtctgctgc ttgtctactg gaccatcaag cgctttttcc acggcgacca ctcccgcgcc
     1381 tttgtcaccg cgctcgtcct gatgctcacc cccttctact acctcatcac caagcagttc
     1441 atgtgggata tcacggcggt gaccttcatc tacggcggcg tcatgttcct cttcctcggc
     1501 atgcgcgacg atgaccgccg actggtgcgg ctcgcctatg tgttctgggg actcgccatg
     1561 ctcgccaaag gactgctcgc ggtcgtcata ccgggcggca tattcttcct ctggatgctc
     1621 gcgaccgccg actacggcct ccttgccgag aagggcggcg ttaaaaaggt cttccagcag
     1681 tacggcgact tcctgaaaaa ggcccgcatc ctcgagggga tcggcctgtt cctgatcgtc
     1741 tcggcgtggt ggtacctcta catgtacgcc aagcacggtc tcccgttcct caaggagttc
     1801 tttgtcgaga accacttcgg tcgcatggaa gggctcatcg acaagcccga cggcccgttc
     1861 gagttctatc tgtggcagat agcgatcggc gccttcccgt ggttcgcgct attcattccc
     1921 gggctcatct tcgccggcga ccataaagag cacaagcgcg aagagggctt catcatcctc
     1981 tcgttcttct tcgtcttcct gttcttcacc ctctcggcga cgaagttccc ccattacatc
     2041 ttcccggcga tgcccttctt cgccgccatc gtcgccattc ccatcatcga gttcttccgg
     2101 ggcgagcgac gcaccctcta cccgctcgcg gcagtgcttg gcgccctcat cctcggggtg
     2161 accggtaagg atctcgggac cggcatcaac taccgcgaac tgctctacat catcacgacc
     2221 caccggatac aggactggtt cggtcgcgtc tacgacatgc tcccgtggct ctacgcgctc
     2281 gtgccgccga tggtcttttt cctgctcctg ccgctcgtcc gtttgaaaag cctcctgctc
     2341 atgcggatat cgctcgtggg cttttcgctc gttgcggtcg ccttcgcctc ctatatcaac
     2401 ttctactggg tcccgaaggt gctctgggta ttcaccccca agcaactggt cgagaagtat
     2461 ctcgaggtga aggagccggg cgacctcatc gtcgactacg acaactggaa gaatcgttgt
     2521 ctctatttct acctcggccc cgacgagcaa ctcatccggg ccaacacacc ggcggcggtc
     2581 aagaaactca tagaggacaa cccgacccac gccgtctaca tcaccaccaa gaagaacaag
     2641 gtccccgaac tgcgatcggt gctcatggca tcgggcgtgc cgctcaccaa gatcatggac
     2701 gacgcggtcg acacctacat ggaggtggag atgtaccgcg cctcgatgaa ggagaagggc
     2761 tcgtccgacg acagttggaa aaaggatctc atcaccgaag acaagatacc gaacaacatc
     2821 cagcggaccg ggtcgacgct cgatgacaaa acggtcgaga tcatcggcta taaactcaac
     2881 aagaactact tcgaacaggg agagaagatc gtcatcgaca tgtattaccg cgtgctcaag
     2941 accaccgaga agaactggca gatattcttc catctcgatg tcttcgaggg ggcgttgccg
     3001 cacagcatca agatagacga atacccgctc aagggtttct acccgaccga caaatggcag
     3061 gtcggcgaca tcatccacga ccagttcgag gcgaccgtcg cgcgcggcca ccccggcggc
     3121 ggtatcaaga tatacaccgg cttctacatc ggcaacgacc gcatgaaggt cgacgaacag
     3181 aagttcaacg acggcgtcaa ccgtttcatc ctcggtacct tcaacatcaa ggtcaagtag
     3241 ccccgtgcgc ctcctttcct tcctcctttt caccgctatc gctgtttcct gcggggataa
     3301 cggtcgcggg acaccgctca acaccgacga ccttccggcc gaagaggacc tgttcgatct
     3361 atcggacggg tctgatctac cggacctttc cgatctgccg gatcagaccg accagtccga
     3421 accggaggaa caggatgatc tgatggaact tcccgacgaa gacgagggac ctgatctcac
     3481 ggtgaacgac atagaccatt cgctcttcgg acaggcgggc gcctgcggca cggtcccggc
     3541 gacgcccggc gtgacgaccg acgcctgcgg cttcatcgtg aacggcgcct a
//
LOCUS       DAOQUE010000285         3571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_29742_length_3571_cov_3.626280, whole genome shotgun sequence.
ACCESSION   DAOQUE010000285 DAOQUE010000000
VERSION     DAOQUE010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29742_length_3571_cov_3.626280"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1471
                     /locus_tag="PKH10_08905"
     CDS             53..1471
                     /locus_tag="PKH10_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT28280.1"
                     /translation="MVVRTREDQNIEWKESWRDEHLKWISAFANAEGGVLVIGKNDSG
                     TVVGVANAKKLLEDIPNKIRDILGIVVNVNLRKSGGREYLEIIVEPHPYPVSYKGEYH
                     YRSGSTKQELKGVALDRFLLRKYGKRWDGVPVPGFSPADCSQSAFQLFKAKAAKSGRM
                     SEDVLQDTDLSLLENLQLTEGNYLKRAATLLFNENTEKFVGGSYIKIGFFVTNDDLRY
                     QDEIQGNLFAQVEKTLDLLFSKYLKAHIRYEGIQRVEEFLFPRPALREAILNAVVHKD
                     YSSGVPIQLSVYEDRVVLWNPGQLPEDWTLQRLLGKHPSNPYNPLIAGAFFRAGYIES
                     WGRGIEKIEKECRVHGIRPPEYDYGMSGLMLTFQADPEHLRRARGDEDPSTEVGGKTP
                     VKTPVKTPVKTPVKTPVKTPVKILQLLQENPSMTLAEVAREIGKSLSAVERASTKLVK
                     EGRLRYVGPQKGGHWEVMKVDE"
     gene            1563..2615
                     /locus_tag="PKH10_08910"
     CDS             1563..2615
                     /locus_tag="PKH10_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28281.1"
                     /translation="MPRNETFGTVLLLDIMNSSEYANVLSVKDYYETFLREFNDTVLR
                     AAEMYFRLYPGGAYDATNHYVEVVGDEGRIFLFSDNPGRDVQTALDLAVLIKIAWING
                     PFNRARIFQNKAPEDLGAGINSGYLYIKKNGQLEGFGINLTKRIESHTRFGSVSKIML
                     HKTSRSILDRYVTENRIAMRLPVNGKDISLPQHYFFRFAGNIELKGIAQPVPIYELQY
                     INWGDRPFFMESSFIKALAKRSVADFTNILETALSLSVLDTTINNILLFLALMNGDWE
                     KASRVAKTLYEELDNIMEIPLVAAVANINLARGTTKDAARRCLLYGRRWLEIARTIRP
                     HAEQIDELEEILSAFR"
     gene            complement(2633..3373)
                     /locus_tag="PKH10_08915"
     CDS             complement(2633..3373)
                     /locus_tag="PKH10_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNT28282.1"
                     /translation="MTSPQDIARVPRHVAVIMDGNGRWAKKRMWNRLRGHREGAESVD
                     IVTEAARMAGVEYLTLYAFSTENWNRPADEVSGLWELLGDFLEKKKAKLINNRIRLHF
                     IGHMEHLPEKTREKALRVKKDTEENGWDMTLTLALSYGSRDELTCALRAVAEKVQRGA
                     LRPEDITEDLISEHLFTAGLPDPDLIIRTSGEVRISNFLLWQSAYAEYYFTDVLWPDF
                     REKEFLEALRSYAGRTRRFGKTDEQLKD"
     gene            complement(3389..>3571)
                     /locus_tag="PKH10_08920"
     CDS             complement(3389..>3571)
                     /locus_tag="PKH10_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28283.1"
                     /translation="PHDTIHDKQARRFALLAQKAITEGSLKQAMTQLKMANSMEPGNE
                     YILSLMEDVQKKMNAG"
BASE COUNT          776 a          996 c         1066 g          733 t
ORIGIN      
        1 tatcttttcg gagaattagt tttgtcttga tctttgggga ggtgtcgaga aaatggtggt
       61 ccggacaaga gaagatcaga acatcgagtg gaaagaatct tggcgcgatg aacacctgaa
      121 atggatctcc gctttcgcca atgccgaagg cggtgtactg gtcatcggca agaacgatag
      181 cggtactgtg gtcggcgtgg ctaacgcgaa aaaacttctc gaagacattc ccaataagat
      241 acgcgacatc ctcggcatcg ttgtgaatgt taatttgcgg aaaagcggtg gacgggaata
      301 cttggagatc atagtggagc cgcatcccta tccggtcagc tataagggag aatatcatta
      361 tcggagcggc agcaccaaac aagaactcaa gggggtcgct cttgatagat tccttctgcg
      421 caaatacgga aaacgatggg atggcgtgcc ggtccccgga ttctctccgg ccgattgcag
      481 ccaatccgcg tttcagttgt tcaaagcaaa ggccgccaaa agcggtcgca tgagcgaaga
      541 tgttttacag gacaccgacc tttcgcttct cgagaacctg caactcaccg aagggaacta
      601 ccttaagcgg gccgcgactc ttctatttaa tgaaaacacc gagaagttcg tcggtggatc
      661 ctatatcaag atcggtttct ttgtgaccaa tgacgacctc cgttatcagg atgagataca
      721 gggcaatctg tttgcacagg ttgagaaaac gctggacctg cttttttcca aatatctgaa
      781 ggcccacatc cggtacgaag ggatccagcg tgtggaggag ttcctcttcc cccgtccagc
      841 cctgcgagag gcgatcctca atgcagtggt gcataaagac tacagcagcg gggtcccgat
      901 ccagctcagt gtctatgaag accgggtcgt gctttggaac cctggacagt tgccggagga
      961 ctggacacta caacgactct taggtaaaca tccgtcgaat ccctacaatc cgcttattgc
     1021 cggagctttc ttccgcgccg gttatataga atcgtgggga cgcggtattg agaagatcga
     1081 aaaggaatgt cgcgtacacg gcatcaggcc gcccgaatac gactacggta tgtctggatt
     1141 gatgcttacc ttccaagccg atccggaaca tttgagaaga gcacggggcg atgaggaccc
     1201 gtcgacagaa gtgggcggaa aaacgccggt gaaaacgccg gtgaaaacgc cggtgaaaac
     1261 gccggtgaaa acaccggtga aaacaccggt gaaaatcctt cagttgctac aagagaaccc
     1321 gtctatgacc ttagccgaag ttgcccgcga gatcggcaaa tcgttgagcg cggtcgagcg
     1381 cgccagcacc aagttggtga aggaaggtcg cttgcgatat gtcggaccgc aaaaaggcgg
     1441 tcactgggaa gtaatgaaag tcgatgaata gcgtgggggc accgaccgat tccactttcc
     1501 cgaaaatctt gtcaaacggg ggcgcggtcg ctatgatggg cccggatcac acggagccgg
     1561 ccatgccacg caacgagacc ttcggaacgg tgcttctgct cgacatcatg aattcctccg
     1621 agtacgccaa tgtgctctcg gtcaaggatt actacgagac cttcctgcgc gagttcaacg
     1681 acacggtcct gcgcgccgcc gagatgtatt tccggctcta tcccggcggg gcgtacgacg
     1741 cgaccaacca ctatgtcgag gtggtggggg acgaggggcg catattcctg ttctccgaca
     1801 acccggggcg cgatgtccag acggcgctcg acctcgcggt gctcatcaag atcgcgtgga
     1861 tcaacgggcc gttcaaccgg gcgcgcatat tccagaacaa ggcgcccgag gacctcggcg
     1921 ccggcatcaa ttcgggctac ctgtacatca agaagaacgg gcaactggaa gggttcggca
     1981 tcaacctgac caagcggatc gagagccaca cccgcttcgg gtcggtcagc aagatcatgc
     2041 tccacaagac ctcccgttcg atactcgacc gctatgtgac cgagaaccgc atcgccatgc
     2101 gcctgccggt gaacggcaag gacatatcgc ttccgcagca ttatttcttc cggttcgccg
     2161 ggaacatcga actgaagggt atcgcccagc cggtgccgat atacgaactg cagtacatca
     2221 actggggcga tcgcccgttc ttcatggaga gttccttcat caaggcgctc gcgaaacggt
     2281 cggtggccga tttcaccaac atcctcgaga cggcgctgtc gctgtcggtg ctcgacacga
     2341 cgataaacaa catactcctg ttcctcgccc tcatgaacgg cgactgggaa aaggcgtccc
     2401 gcgtcgccaa gacgctgtac gaagaactcg acaacatcat ggagataccg ctcgtcgcgg
     2461 cggtggcgaa catcaacctt gcacggggca ccacgaagga tgccgcccgc cggtgcctcc
     2521 tgtacggccg ccggtggctc gagatcgcgc ggaccatccg gccgcacgcc gagcagatag
     2581 acgaactcga ggagatactg tcggccttcc gttgaaccgc ggtcccgcgg cgttaatctt
     2641 tcaactgttc gtcggtcttg ccgaaccggc gggtgcgccc ggcgtatgac cggagcgcct
     2701 cgaggaattc cttttcgcgg aaatcgggcc agaggacatc ggtgaaatag tattcggcgt
     2761 aggcgctctg ccacaacagg aagttgctga tgcgcacctc gcccgaggtc cggatgatga
     2821 gatcggggtc ggggagcccc gcggtgaaga ggtgctcgct tatcaggtcc tcggtgatat
     2881 cttcgggacg cagggcgccg cgttgcacct tttcggcgac ggcgcgcagg gcgcaggtca
     2941 gttcgtcccg cgaaccgtag gaaagggcga gcgtgagcgt catgtcccag ccgttctcct
     3001 cggtgtcctt cttgacgcgg agcgcttttt cgcgagtttt ttcaggcaga tgttccatat
     3061 gaccgatgaa atggagccgg atgcggttgt tgatgagctt cgcctttttc ttctcgagga
     3121 agtcgccgag cagctcccac aggccgctca cctcgtcggc ggggcggttc cagttctcgg
     3181 tggagaaggc gtagagcgtg aggtattcga cgcccgccat acgggccgcc tcggtcacga
     3241 tatcgacgct ctcggccccc tcgcggtggc cgcgcagacg gttccacatc cgtttcttcg
     3301 cccagcggcc gttgccgtcc atgatgacgg cgacatgccg ggggacgcgc gctatgtcct
     3361 gtggggaggt caccggcgat ccttccggct acccggcgtt catcttcttc tggacatcct
     3421 ccatgagcga gaggatgtat tcgttccccg gttccatgga gttggccatc ttgagttggg
     3481 tcatcgcctg tttgagcgag ccttcggtga tcgccttctg ggcgaggagc gcgaagcgtc
     3541 gtgcctgctt gtcgtggatg gtgtcgtgcg g
//
LOCUS       DAOQUE010000286         3539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_30142_length_3539_cov_4.068599, whole genome shotgun sequence.
ACCESSION   DAOQUE010000286 DAOQUE010000000
VERSION     DAOQUE010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30142_length_3539_cov_4.068599"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1192)
                     /gene="tgt"
                     /locus_tag="PKH10_08925"
     CDS             complement(95..1192)
                     /gene="tgt"
                     /locus_tag="PKH10_08925"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNT28284.1"
                     /translation="MIRFTVEKRDGHSRAGIIETPHGAVPTPTFMPVGTKGSVKGVLP
                     QQLREAGVGIVLGNTYHLFLRPGHELVRETFGSLHRMIGWDGPILTDSGGFQVFSLGP
                     LRKITEEGVEFRSHLDGSKQFLSPERSVEIQEALGADIMMAFDECPALPCSDNEMLAS
                     MERTTRWERRCLEARKGDAALFGIVQGGVSEALRRKHLAEITALDFDGYALGGLSVGE
                     AKEDMYRTVAAIAPVMPDDRPRYLMGVGTPRDLVAAILSGIDMFDCVMPTRNARNGQL
                     FTRGGTVNIKNKEFERDTRPIDERCTCLTCRSFTRAYLNHLYKNGEILAAVLNSIHNI
                     HYYLDLTATMRRLILEGRAVEGFALVATYDR"
     gene            1305..1994
                     /gene="radC"
                     /locus_tag="PKH10_08930"
     CDS             1305..1994
                     /gene="radC"
                     /locus_tag="PKH10_08930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNT28285.1"
                     /translation="MDKKGHNGVGHRQRLRDRFLRTGLEGFSEHEIVELLLTLAIPRR
                     DVKPAAKELVRRFGGLRGLLDASIEDVRAVPGIGEVAPVALKIVRETAALYLRKEGES
                     APFLGDVGKLVDFWRMRIGGLRHEAVQVAYLDAGYRLIREGVETLAEGTVDRAALYPR
                     RVVESALRRGAAAIVLAHNHPNGVSTPSDADTVLTRAIVRALDTVSIRLLDHIIVAGD
                     THFSFRNAGLL"
     gene            complement(2049..2219)
                     /locus_tag="PKH10_08935"
     CDS             complement(2049..2219)
                     /locus_tag="PKH10_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT28286.1"
                     /translation="MAHKIDAGACISCGACAAECPVDAISQGDSSYVIDAAKCTDCGA
                     CKGVCPVECISN"
     gene            complement(2297..3358)
                     /locus_tag="PKH10_08940"
     CDS             complement(2297..3358)
                     /locus_tag="PKH10_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28287.1"
                     /translation="MWVTCLFPEQLGLLAGAKVHLPQCLSCPVTGCPRKADAFRSFFT
                     EQVALSSSSLNRKIMDLNEKLDSAKKRGDLYCPRKGSLTTKCSGLSSFCRYNLICGRF
                     KERTGRLFIRSLRWRRTMVYVVKFRNGEVRQIEKGDLATLNIDLVEVVYPGTTRVEVV
                     TELVPQGEEQERLSDTIASFREKYNGKVVCDRGMVDFGEWFASAATGDHALIPERALV
                     PLKTYRIVKIKGGEKVKPPVQSPVPVEVAQLSAPRQITPPTPPIASPKPLPGKKPTPV
                     TVEKKVADPKATKQPPAPKPEKKEREKEVVAQKKGPGRPPKKKENAEDVQPRLFTPSS
                     PARRPNKLKIDKKNTNKKK"
BASE COUNT          637 a         1013 c         1112 g          777 t
ORIGIN      
        1 agcgccgggt ctcttgttgg atcgacacgg gtgccccccc ctcctgcaga acggcccact
       61 atatacgggg cgggggggta ttgcaatttt tcggctatcg gtcgtaggtc gccacgagcg
      121 cgaaaccttc cacggcgcgt ccttcgagga tgagtcgtcg catcgtcgcg gtcaggtcga
      181 ggtagtagtg gatgttgtgt atcgagttga ggaccgcggc gagtatctcg ccgttcttgt
      241 agagatgatt gaggtaggcc cgcgtgaacg accggcaggt aagacaggtg catcgttcgt
      301 cgatgggacg ggtgtcccgc tcgaactcct tgttcttgat gttcaccgta ccgccgcgcg
      361 tgaagagttg gccgttgcgg gcgttgcggg tcggcatcac gcagtcgaac atgtcgatgc
      421 ccgaaagtat cgcggcaacg aggtcgcgcg gcgtgccgac ccccatgaga tagcgcggcc
      481 ggtcgtcggg catgaccggg gctattgcgg cgacggtgcg gtacatgtcc tctttcgcct
      541 cgccgaccga cagcccgccg agtgcatagc catcgaagtc gagagcggtg atctcggcga
      601 gatgcttgcg gcgcagtgcc tcggaaacgc caccctgcac gatgccgaaa agcgccgcgt
      661 cgccttttcg ggcctcgaga caacgtctct cccagcgggt cgtccgctcc atgctggcga
      721 gcatctcgtt gtcggagcag gggagcgccg gacattcgtc gaacgccatc atgatgtcgg
      781 cgccgagcgc ctcctgtatc tcgacggagc gttcgggcga gaggaattgt ttcgagccgt
      841 cgaggtgcga acggaactcg accccctcct cggttatctt ccggagaggt ccgagactga
      901 acacctgaaa gccgccagaa tcggtgagta tcgggccgtc ccagccgatc atgcgatgaa
      961 gcgagccgaa ggtctcgcga acgagttcgt gtcccgggcg caggaagagg tggtaggtgt
     1021 tgccgagcac gatgccgacg ccggcctcac ggagttgctg tgggagcact cccttgacgg
     1081 aacccttggt gccgaccggc atgaaggtgg gggtcggcac cgcgccatgc ggcgtctcga
     1141 tgatgccggc gcgagagtgg ccgtcacgct tttccacggt gaatcgtatc acaggtgcct
     1201 ccggtgttcg atggcattat agcgacggaa tgcgacaggt cgagattttt tgacagagga
     1261 gtgctccccc gctagcatgg cgcgaccgga cgacggggtg gtgcatggat aaaaagggac
     1321 ataacggtgt tggacaccgg caacgcctgc gggaccgttt cttgagaacg gggctcgaag
     1381 gattctccga acatgagata gtcgaactgt tgctcacgct cgcgataccg cggcgcgatg
     1441 tcaagccggc ggctaaggaa cttgtccggc ggttcggcgg cctgcgcggt ctgctcgacg
     1501 cttcgatcga agatgtccgc gcggtgcccg gtatcggcga agtggcgccg gtcgccctca
     1561 agatagtccg ggagaccgcg gcgctctatc tccgcaaaga gggtgagtcg gcccccttcc
     1621 tcggcgacgt ggggaaactc gtcgatttct ggcggatgcg gatcggcggt ctgcggcacg
     1681 aggcggtgca ggtcgcctat ctcgacgctg gataccgatt gatccgcgaa ggggtcgaaa
     1741 cgcttgccga agggacggtc gatcgtgcgg cgctttatcc gcgccgggtc gttgaatcgg
     1801 cgttgcgtcg cggcgccgcg gcgatagtgc ttgcgcacaa ccatccgaac ggggtctcga
     1861 cgccgagtga cgccgatacg gtgctcaccc gcgccatcgt ccgggcactc gacaccgttt
     1921 cgatacgtct gctcgatcac atcatcgtgg cgggtgatac ccacttcagt ttccgtaatg
     1981 cgggactcct gtaactcaaa aaaaagcccc cgaggaccgg gggctcattt gggggggggg
     2041 taaatccttt agttcgagat gcactcgacg gggcagacgc ctttgcacgc accgcagtcg
     2101 gtgcacttgg cggcgtctat cacataggaa ctgtcgccct gactgatcgc atcaacgggg
     2161 cattcggccg cgcaggctcc gcaactgatg cacgcgccgg catcgatctt gtgcgccatg
     2221 agaacctcct cttattctaa aaaggataac acccgcatgc tctatcatat tcccgtacaa
     2281 gagtcaacta gcccgctcat tttttcttgt tagtgttttt cttgtctatt ttcaatttgt
     2341 tggggcgtct ggcgggcgag gacggggtaa aaagtcgtgg ctgaacatct tcggcattct
     2401 ctttcttttt tggtggtcgt cccggtcctt ttttttgtgc gaccacctcc ttttccctct
     2461 cttttttctc cggcttggga gccggtggct gttttgttgc ctttgggtcc gcgactttct
     2521 tttcaacggt gacgggagtc ggcttttttc ccggtagcgg tttgggagat gctatgggag
     2581 gtgtcggcgg ggtgatctga cgcggtgcgc tcaactgcgc gacctcgacg gggacgggcg
     2641 actgcaccgg gggcttcacc ttctctccgc cctttatctt gacgatacgg tatgtcttga
     2701 gcgggacgag cgcccgttcc ggtatcagcg cgtggtcgcc ggtcgccgcc gaggcgaacc
     2761 attcgccgaa atctaccatg ccgcggtcgc agacgacctt cccgttatat ttttcgcgaa
     2821 acgaagcgat ggtgtctgac agtcgttcct gctcttctcc ctgcggtacc agttcggtca
     2881 ccacctccac ccgcgtcgtg ccgggataga ccacttcgac aagatcgatg ttcagtgtcg
     2941 ccagatcgcc cttttcgatc tgccgcacct cgccgttcct gaatttcacc acatacacca
     3001 tggttctcct ccatcgtaac gatcgtataa aaagtcgccc tgttcgctct ttgaaacggc
     3061 cgcatatcag gttgtagcgg cagaacgagg aaagtcccga acatttggtc gtcagagatc
     3121 ccttgcgcgg acagtagaga tctccgcgct ttttcgccga gtcgagtttc tcgttcaggt
     3181 ccatgatctt gcggttcaga ctggaggagg aaagagccac ctgttccgtg aaaaatgatc
     3241 ggaaggcgtc ggctttccgc ggacagccgg tgaccgggca ggagaggcac tgcggaagat
     3301 ggaccttcgc ccccgcgaga agcccgagtt gctcgggaaa aaggcaagtg acccacatgg
     3361 ttccggacac tatcgaccgc cgcataccgt tctgcggggc agtatatcct gaccgtcccg
     3421 gagcgcaaga aaaaaggaag cgacggttgt tgacttttag gtcggacctg ctatagtcga
     3481 acccgagttt agcgtaattt cttgtcgcgg aggtaacaat gcgcagttat ttcagtcgt
//
LOCUS       DAOQUE010000287         3531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_30233_length_3531_cov_4.548907, whole genome shotgun sequence.
ACCESSION   DAOQUE010000287 DAOQUE010000000
VERSION     DAOQUE010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30233_length_3531_cov_4.548907"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1625)
                     /locus_tag="PKH10_08945"
     CDS             complement(147..1625)
                     /locus_tag="PKH10_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28288.1"
                     /translation="MRHFLFVPLISALLSYFLAACDKKNTAQAEGSLATDDSHITDHE
                     YLQGDDRGDISSDIDVPAVNGPVWTITESDISWHKQDNVFFHQWGTAAIDAAMKIYKF
                     GDQVAVLSRYNISQEYHDQMMLSLITGGNHIKNYLLGSTFPARLFANEATQGKDGRIF
                     ITAISSADLGKTFEKNDKQKYDACVIVIDPKDDSFSFFQWGNEGYAHPETILINDDGS
                     LTVAGFTDGAFPGQELKGKSDIFISRIETKTGRISTVQYGNGTPTIPSTPRLIGMDKN
                     YWHFALMPETALPGQTFFGGPWDAGIIAIDRKIGFQRYWQFGTDNMDFFGSTILDEKE
                     RMHFIGTTFGTFAGQKHIGDSNYSDLFYGYFENGKVAIVMQYGTDLDEMNGALRLHKG
                     TLYHTGSTSGACENAYAAPKKDILFAKIDPATKKMTCVKFGDVAEDDLGYDLLVEDDG
                     TVYIAGNTQGAVGKNSYHLDEKNAPRSDAFLMKTTLKDLGFE"
     gene            2162..2347
                     /locus_tag="PKH10_08950"
     CDS             2162..2347
                     /locus_tag="PKH10_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28289.1"
                     /translation="MVLGATLSGRLSPRYVCPKKEKGSIRNTSSINRLALPDGMMFMT
                     APLKRTVMRVGQFFGTG"
     gene            2407..>3531
                     /locus_tag="PKH10_08955"
     CDS             2407..>3531
                     /locus_tag="PKH10_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28290.1"
                     /translation="MRRYGKIFLAAALFGVLFLWATRGLFWTPLTAFFPQFHLLVLAT
                     LLLCVGMALKGYGLWRAVCETVMARRNFMFPPVAFLAPLVLALFFFNETAHPMDAAHY
                     LWTAKLFLSGQLYLPLPPFYEHAYEGFMVVHDGRYYSLFPPGFPALLAPFAALGLAFW
                     LNPILNGVAVYLTGRVAEELTGDRRVAVLTMLLTTVSSFHLFLSATLFPHPAILVLTL
                     LALFVALRGELSAGRVAAVAIIIGLIAPVRPQDGLFTAAAFGVFLLLRGMRITPATML
                     AFLAPLALGALMLLGYNRAVTGEWFVFPQDIYFAVTEESARCHRIGLGTGCRHLNGLF
                     LPPEGLTVKYAFFVTMTRLSMLLYKTTLHPLMFLFAVAAAW"
BASE COUNT          649 a          977 c          982 g          923 t
ORIGIN      
        1 cgactcaccc ggaaggggta cggccggtcg ttcctcgacg cgctccccga cgggatgatg
       61 ttcatgaccg cgccgctgaa acgggtggtg gggcgggtcg gccagttctt cggtcacgga
      121 taatcgggaa aaatcgtttc ggcggattac tcgaaaccga ggtctttgag agtcgttttc
      181 atcaggaaag cgtcggaacg cggagcattc ttttcatcca aatgataact gttttttcct
      241 accgcccctt gagtgttgcc cgcgatgtaa accgtgccgt catcctcgac aagaaggtca
      301 taccccagat cgtcttccgc gacatcgccg aattttacgc aggtcatttt cttggtggcc
      361 gggtctattt ttgcgaaaag aatgtctttt ttgggggcgg cataggcgtt ctcgcaagca
      421 ccacttgtgc tccccgtgtg gtacaatgtt cctttgtgta gacgcaaagc gccgttcatt
      481 tcatcgagat cggtcccgta ctgcataacg atagcaactt ttccgttctc gaagtacccg
      541 tagaaaagat cgctgtaatt cgaatctccg atgtgctttt gtcccgcgaa ggtcccgaag
      601 gttgttccga tgaaatgcat tctttctttt tcgtcgagga tggtcgatcc gaaaaaatcc
      661 atattgtcgg ttccgaactg ccagtatcgt tggaatccta tcttgcgatc gatagcgatg
      721 atgccggcgt cccagggccc cccaaagaag gtttgccccg gtagcgccgt ttccggcatg
      781 agggcgaagt gccaatagtt tttatccatt ccaatcagtc tgggagtact gggaatggtc
      841 ggggtgccgt taccgtattg taccgttgaa atacgaccgg tctttgtctc tatgcgtgaa
      901 atgaaaatat cgcttttgcc cttcagttcc tgccccggaa aagcgccgtc ggtaaaaccg
      961 gcgaccgtca gagaaccgtc atcgtttatc agtatggttt ccggatgtgc gtacccctcg
     1021 ttcccccact ggaagaagga aaaagaatca tctttcgggt caatgactat gacgcaggca
     1081 tcgtatttct gtttatcgtt cttttcgaag gtttttccga gatcggcgct gcttatggcg
     1141 gtgatgaata ttctcccgtc tttcccctga gtggcctcgt tggcgaagag gcgcgccggg
     1201 aaagtgctcc ctagtagata attctttatg tgatttcctc ccgttatgag agatagcatc
     1261 atctgatcgt ggtattcctg gctgatattg tagcgactga ggaccgccac ttggtcgccg
     1321 aacttgtata ttttcattgc tgcatctatc gccgcagtgc cccactgatg gaaaaagaca
     1381 ttgtcttgtt tgtgccacga gatatcactt tcggtgatgg tccagaccgg accattcacc
     1441 gcggggacat caatgtcgga agatatatcc ccccgatcat cgccctgaag atactcgtga
     1501 tcggtgatat gagaatcgtc cgtggcgagg gacccttctg cctgtgcggt attcttttta
     1561 tcgcaggcag cgagaaaata cgaaagaagg gccgagatca acggaacgaa aagaaaatgt
     1621 cgcatatctt ccctccgtaa agaacccatg cttgtaattt ttacttgcaa tttttttttt
     1681 tcaattattt cagctttgtt gcgggtgctt ttttacgaca taactaaaaa agtaatggag
     1741 gagaacatga gaaaaggtat gatcttcatg gtggtgttct tgacggccca atttcttttc
     1801 gcggtaggga cgcctacggt gaacagttgt ttcatccctg ggtcgagctg gggtcttttg
     1861 gcctgtcaat ggttcagttg tggatggtcg gccgtgtccg gcgccgatca atatgagtgg
     1921 gaggcctaca ccaacggttg gaccggcttc ccttgcgacc agtggtatcc ggggtccaca
     1981 acgtcttgcc ttaccaatac ggataccgtt ccgtcgtccg aaacggattt cattgatccc
     2041 gaaatgacca tgacgggcag tacaacggcc aaggcgggac gggctagagc gatagactat
     2101 gtgcctacgc agatatggaa ccctgccctg cgacagtggg taactattta tttgccttcg
     2161 tatggtgctt ggagcaacac taagtgggcg tctttcccca cgatatgtat gccctaagaa
     2221 agagaaggga agtatccgca acacttcttc aatcaataga ctcgcgctcc ccgacgggat
     2281 gatgttcatg accgcgccgc tgaaacggac ggtgatgcgc gtcggccagt tcttcggtac
     2341 cggctagcgg cggaaccttc ttcttcccgg ggcggtctat cgtagtaact gtcagcgtgc
     2401 gggatcatgc gaaggtacgg taagatcttt ctggcggcgg cactgttcgg ggtgctgttc
     2461 ctgtgggcga cacggggcct gttctggacg ccgctgaccg cctttttccc gcagttccat
     2521 ctgctcgtgc tggcgacgct cctcctctgc gtcggtatgg cgctgaaagg gtacggtcta
     2581 tggcgggccg tctgcgaaac ggtgatggcg cggcgcaatt tcatgtttcc gccggtggcc
     2641 ttcctcgcgc cgctcgttct cgcccttttc tttttcaatg agacggcgca cccgatggat
     2701 gcggcccatt atctctggac cgcgaaactg ttcctttccg ggcaactgta tctgccgctc
     2761 ccgccgttct acgagcatgc ctacgagggg ttcatggtcg tgcacgacgg ccggtactat
     2821 tcgctcttcc cccccggttt ccctgcgctc ctcgcgccgt tcgccgcgct cggtctcgcc
     2881 ttctggctca acccgatact caacggcgtt gcggtctacc tgaccgggcg ggtggccgag
     2941 gaactgaccg gtgaccggcg ggtcgcggtg cttacgatgc tcctcacgac cgtgtcatcc
     3001 tttcatcttt tcctttcggc gaccctcttt ccccacccgg cgatactcgt gctgacgctc
     3061 cttgcgcttt ttgtcgcctt gcgcggcgaa ctttcggccg gtcgcgtcgc ggcggtcgcg
     3121 atcatcatcg ggctcatcgc gccggtacgg ccgcaggacg gcctgttcac tgcggcggcg
     3181 ttcggggtct ttctgctcct gcgggggatg cgtatcacgc ccgcgacgat gctcgccttc
     3241 cttgcgccgc ttgcgttggg cgcgctcatg cttctcggtt acaaccgcgc ggtgaccggc
     3301 gagtggttcg ttttcccgca ggatatctat ttcgcggtga ccgaggagtc ggcgcgctgc
     3361 caccgcatcg gtctcgggac cgggtgccgc cacttgaacg gcctgttcct cccgcctgag
     3421 gggctcacgg tgaagtatgc ctttttcgtg acgatgacgc ggctctcgat gctcctctac
     3481 aagacgacgc tccacccgct catgttcctg ttcgccgtcg cggcggcgtg g
//
LOCUS       DAOQUE010000288         3530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_30249_length_3530_cov_5.472806, whole genome shotgun sequence.
ACCESSION   DAOQUE010000288 DAOQUE010000000
VERSION     DAOQUE010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30249_length_3530_cov_5.472806"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKH10_08960"
     CDS             <1..358
                     /locus_tag="PKH10_08960"
                     /inference="COORDINATES: protein motif:HMM:NF023367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNT28291.1"
                     /translation="DNRRMMLFGDDLNEHQQDTLAVVKKLAAARAAYPALRRGTRETV
                     HVEEDVWLYTMSDGDDTVLIAFNRGEETVGRTFAVPLVDGSYTDALSEGTTVVKEGSI
                     DLTLPPRSAAIYAKAE"
     gene            complement(359..1291)
                     /locus_tag="PKH10_08965"
     CDS             complement(359..1291)
                     /locus_tag="PKH10_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28292.1"
                     /translation="MRGFLLFIACFAIVLTAACDNATRTLVDKDTTVTDTTGDTDADT
                     VQTDDIQTDDVQTEGIQTDDIQTDDLQTDDILPDTQGDGDTVQNDQNVPDNDMPPKTC
                     ADITCDDPNSACVEGTGGADAKCECNEGYHWNSGQCVKDELTKIGEFSFDFEGAINET
                     TDPDQLQGGDGTATFSHLGEDFSYVKITVPYGNIGFPLANLQEGTTITTIWLEKLAMS
                     GSVKFFAFSMPAAQNTAGTKQFANTQSVVMYGDMTMSGGAMNIECVRAMSADGSYTVA
                     GDSGNGTLHISTASGNMYDPKVAEANLPAPICEE"
     gene            1451..2779
                     /locus_tag="PKH10_08970"
     CDS             1451..2779
                     /locus_tag="PKH10_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HNT28293.1"
                     /translation="MRLLVTGSFCLVAVLFAAACENRTLSRPLDADQAATDDGTVIAD
                     DISGDTDDLLSDGTPDETLPDEASDEERPDGDTLEAVCIENGDCSDGLFCLREAPTLC
                     EGSGACAVQPVACGEEYAPVCGCDGKSYPNECSAWAAGMNIRHGGPCGSMIPCTANKQ
                     CTETEYCAKPLGVCDADDSGLCDLRPIDCDLITEFAFACGCDDATYLHPCHAAAAGVN
                     VAHPGKCDGENICLYNDECGQTEFCRKAEGVCGQSSGACEARPEACPFSFDVGAPVCG
                     CDGVTYADECSAWISGMNIAFTGACEDAIPKSSLTYSYRKGEDPEVTAHIFVALSVGE
                     TAEYFGASVVEKDNSGIANILLKVTYGGGMPGEEAIMVQLSLPRTANVPYAVTIDGAG
                     NYVRWLDAMGMPKADLVGTVTITLYETDNDFPNYTVTAVDFFGENLVPKP"
     gene            complement(2783..3289)
                     /locus_tag="PKH10_08975"
     CDS             complement(2783..3289)
                     /locus_tag="PKH10_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNT28294.1"
                     /translation="MIGAKIVLYNVPGRTGLNFPAGWLKELAAIPQITAVKEASGNMA
                     QAVDYFEQVGPDRFAMLSGDDFTITPFIALGGAGVISVVSNIAPAETVALTGAALAGD
                     FGKATSLQVRLNGLIRALFMESSPIPVKTALHLMGFCEEILRSPLATMEPQNREKLVE
                     TLKRYRLV"
     gene            3294..>3530
                     /locus_tag="PKH10_08980"
     CDS             3294..>3530
                     /locus_tag="PKH10_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT28295.1"
                     /translation="MAPWLGLLTGVAAGMLFSLLHAFTSIHLKADQIISGTALNILAG
                     GLTIYLCQILFHQQRTRAFSYGLRKVTVPLLSKIP"
BASE COUNT          624 a         1088 c         1119 g          699 t
ORIGIN      
        1 cgacaaccgc cgcatgatgc tcttcggcga cgacctgaac gaacaccagc aagatacgct
       61 cgccgtcgtg aaaaaacttg ccgccgcgcg cgcggcctac cccgccctgc ggcgcggaac
      121 gcgcgagacg gtccatgtcg aggaggatgt ctggctctac acgatgagcg acggggacga
      181 tacggtgctt atcgccttca accgcggaga agagacggtc ggacggacct tcgcggtgcc
      241 gcttgtggac ggaagctata ccgatgcgct gtctgagggg acgacagtgg tgaaggaggg
      301 gtcgatcgac ctgacgctcc cgccgcggtc ggcggcgatc tacgcgaaag cggagtagtt
      361 attcctcgca gatgggggcg ggcaggttgg cttcggccac cttgggatcg tacatgttcc
      421 cgctcgccgt cgagatatgg agtgtgccgt tccccgaatc gccggcaacg gtgtaggagc
      481 cgtcggccga catggcgcgg acgcactcga tgttcatggc gccgcccgac atcgtcatgt
      541 cgccgtacat caccaccgac tgggtgttcg cgaactgctt ggtgccggcg gtgttctgcg
      601 cggcgggcat cgagaatgcg aagaacttga ccgatccgct catggccagt ttctcgagcc
      661 agatggtcgt gatggtggtc ccctcctgca gattggcgag cgggaagccg atgttgccgt
      721 aggggacggt tatcttgaca taggagaagt cctcgccgag gtgcgagaat gtcgcggtgc
      781 cgtcaccccc ctgcaattga tcggggtcgg tggtttcgtt gatggcccct tcgaagtcga
      841 acgagaactc gccgatcttg gtgagttcgt ccttgacgca ctgacccgaa ttccaatggt
      901 agccctcgtt gcactcgcac ttcgcatcgg cgccgcccgt tccttccacg caggcgctgt
      961 tcgggtcgtc gcaggtgatg tcggcgcagg tcttcggcgg catatcgttg tcggggacat
     1021 tctggtcgtt ctggacggtg tcgccgtcgc cctgcgtgtc gggcaggata tcgtcggtct
     1081 ggagatcatc ggtctggatg tcgtcggtct ggatgccttc ggtctggaca tcgtcggtct
     1141 ggatgtcgtc ggtctggacc gtgtcggcgt cggtgtcgcc ggtcgtgtcg gtcaccgtcg
     1201 tgtccttgtc gaccaaggtg cgcgtcgcgt tgtcgcacgc agccgtcaga acgatcgcga
     1261 aacaggcgat aaaaagcagg aaccctctca tgattacctc cctcgtagtg gttgaacgct
     1321 tgaaacagga gagattgtag aacattggcg tccgaagtca agcggaaaaa accgttcttt
     1381 ttttgcgccg acttgcggaa ctcgtcgtgg tgcgctatac tgacgaccga accgacggtg
     1441 gagggtatat atgagactac tcgtgaccgg gtcgttttgc cttgtcgcgg tcttgtttgc
     1501 cgctgcatgc gagaatcgca ccctgagccg gccgctcgat gccgatcagg cggccaccga
     1561 cgacggtacg gtgatagccg acgatatctc tggcgacacc gacgaccttc tttccgacgg
     1621 gacccccgat gagacgctcc ccgacgaagc gagcgatgag gaacggcccg acggcgatac
     1681 gctggaggcg gtgtgcatcg agaacggcga ctgctccgac ggattgttct gcctccgcga
     1741 ggcgccgact ctctgcgagg gttccggggc ctgcgcggta cagcccgtcg cctgcggcga
     1801 ggagtacgcg ccggtctgcg gttgtgacgg caaaagttac cccaacgaat gctcggcatg
     1861 ggcggctggt atgaacatcc ggcacggcgg tccctgtggc tcgatgatac cctgcaccgc
     1921 caacaagcag tgcaccgaga ccgaatactg cgccaaaccg ctcggcgtgt gtgacgccga
     1981 cgactccggg ctctgcgacc tgcgcccgat agattgtgat ctcatcaccg aattcgcctt
     2041 tgcctgcggc tgtgacgatg cgacctacct tcatccctgc cacgccgccg ctgccggcgt
     2101 caatgtcgcc catccgggga aatgtgacgg tgagaacatc tgcctgtaca acgacgagtg
     2161 cggccagacc gagttctgcc gcaaggccga gggggtgtgc gggcagagtt cgggagcctg
     2221 cgaggcgcgt cccgaggcct gcccgttctc cttcgatgtc ggtgcgccgg tctgtggctg
     2281 cgacggggtc acctatgccg acgaatgctc cgcgtggata tcgggaatga acatcgcctt
     2341 taccggcgcc tgcgaggatg cgatacctaa gtcctctctc acctacagct accggaaggg
     2401 cgaggacccg gaggtgaccg cccacatctt cgtcgcgctc tcggtcggcg agacggccga
     2461 atatttcggc gcgagcgttg tggaaaaaga caattccggc attgccaaca tcctcctgaa
     2521 ggtcacctac ggcggtggca tgccgggcga ggaggccatc atggtccaac tctctctgcc
     2581 gcgcaccgcg aatgtgccgt acgcggtgac gatagacggt gccgggaact atgtccgctg
     2641 gcttgacgcg atgggaatgc ccaaggccga tcttgtcggc acggtgacca tcacgctcta
     2701 cgagaccgac aacgattttc ccaactacac ggtgaccgcg gtcgatttct tcggagagaa
     2761 cctcgtcccg aaaccgtaat ggtcagacca gccggtacct tttgagtgtt tcgacgagtt
     2821 tttcgcggtt ctgcggttcc atggtcgcga gcggtgatcg caggatctct tcgcaaaatc
     2881 ccatgagatg gagcgccgtc ttgaccggta tcgggctcga ttccatgaag agtgcacgga
     2941 tgagaccgtt cagccgcacc tgcagagagg tcgccttccc gaagtcaccc gcgagcgccg
     3001 ccccggtcag cgcgaccgtc tcggcgggcg cgatgttcga caccaccgat atgaccccgg
     3061 cgccgccgag cgcgatgaac ggcgttatcg tgaaatcgtc gcccgacagc atcgcgaacc
     3121 ggtccggccc gacctgttcg aaataatcga ccgcctgcgc catgttcccg ctcgcctcct
     3181 tgaccgccgt tatctgcggt atcgccgcga gttccttcag ccacccggcc gggaagttaa
     3241 gaccggtgcg tcccggcaca ttatagagga cgatctttgc gccgatcacc gcgatggcgc
     3301 cgtggctcgg cctcctgacc ggcgtcgccg ccgggatgct gttctcgctg ctgcacgcgt
     3361 tcacgagcat ccacctcaag gccgaccaga tcatttccgg cacggcgctg aacatactgg
     3421 ctggcggcct gaccatctac ctgtgccaga tcctgttcca ccagcagcgt acgcgcgcgt
     3481 tcagctacgg cctgcgcaag gtgacggtcc cgttgctatc caagataccg
//
LOCUS       DAOQUE010000289         3530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_30252_length_3530_cov_3.821871, whole genome shotgun sequence.
ACCESSION   DAOQUE010000289 DAOQUE010000000
VERSION     DAOQUE010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30252_length_3530_cov_3.821871"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3530)
                     /locus_tag="PKH10_08985"
     CDS             complement(<1..>3530)
                     /locus_tag="PKH10_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28296.1"
                     /translation="AEDPAHKEVAPEARPDNLAYFCISQESSLARGKVTDVNNACVMQ
                     KGPAVTEGGTPSLRIFSADGATELSLGAVVKEPAVLLRFKAESASQAVIGNETGFKTA
                     STKELALTELTSLAEEEGYGTDWYETAWDLNYGRTIETDLTDRAQRTVYLKTMNSVGY
                     ESKPASFELFIDNKPPFIVNRVLDPQKANGGRPVTLSLSFHEPVQGVQVSARHATLTE
                     TAFSLSLNEDISNEQVLVFEYVIGSGGEDAEGDYVLTVEATDAVGNELADELADILTI
                     DKTPPEVTVTGCSVRTRRDLFYTDTFSGEPLSIAAAREGDVVEVTLDITVEDNLDSTG
                     LAPLLTLGSESMVEECETPNAFCWAQTVIHSNTNPEGNKRVLIEAIDSAGNLYSGTVL
                     PTIGGIEKDTCRAIFDFSGPILTSALLQRIPDFAPARDAENKVLSFSLTDPRTDEVVT
                     AQLNLYADEELDPATPAVAGLDFGAAVEVTGNFAQFEKELDDSITAGEHAFTVTWRDL
                     LGNEATRDVDWKMFLDKTLPDAALVDMKKVLYTRKPWGTDDTAGKPKFSVAGESGAVG
                     SADIATIIAYNEQGSIIGQTSVTGGAFNIPTLTGGDLPEIYLNPVKKSGLKAAGNGAL
                     VTEIEWHATMGGKVPGSALENPHTFITISMIDRILEQKGTFLQEPAGGGQEKIARSGD
                     GSWERWKDDNEVPVGREGSAMAYDSVRGKVLLFGGFNGAASTNETWEWDGASWTKLNP
                     LTRPSARAGHAMAYDSARGKVILFGGAIESSNNETWEWDGTNWTQRYPTNKPSARNSH
                     AMTYDSVRGKVVLFGGGDYYDGDSHDYQDTWEWDGANWTQRTSTNKPTARTAHTMAYD
                     SARGKVILFGGASYYDNASHANQDTWEWNGTNWAQVKGPASTICTGACLCTADTCPSA
                     RKGHAMAYDRVREKTVLFGGSSVTEGTWEWDGSNWTQKDSLNKPSARYNHVMAYDSAR
                     GNILMFGGVYSSSYKDETWEWNGTNWTQKNPATKPSARAGHAMAYDSVHEKVLFFGGT
                     FGGNETWEWDGANWALLNPATKPSPRSDHAMAYDNTHGKMVLFGGSSGGNETWEWNGV
                     NWAQIKGTASATCTDTCICTSATCPSARSGHTMAYDGGHEKVLLFGGTNGSTTNNETW
                     AWDGTNWTQIKGVASASCAGSCVCTTDTCP"
BASE COUNT          574 a         1083 c         1032 g          841 t
ORIGIN      
        1 ggggcaggtg tcggtggtac agacgcaaga accagcgcaa ctggcgctgg ccaccccttt
       61 gatctgtgtc cagttcgtac cgtcccatgc ccatgtctcg ttgttggtcg tcgatccgtt
      121 agttccgccg aacaacaaca ctttctcgtg accgccgtcg taggccatgg tgtggccgga
      181 tcgtgcggat ggacaggtgg cagaagtgca gatgcaggta tcggtgcatg tggcactcgc
      241 ggttcccttt atctgggccc agttgacacc attccactcc catgtttcat tgccgccgga
      301 ggatccgcca aataacacca tctttccgtg ggtgttgtca taagccatgg cgtgatcgct
      361 tcgcggcgag ggcttggtcg ctgggttcaa tagggcccag ttggcaccgt cccattccca
      421 tgtttcattg ccaccgaatg tgccgccaaa gaataacacc ttctcgtgga cactgtcata
      481 ggccatcgcg tgaccggctc gtgccgaggg tttggtcgct gggtttttct gcgtccagtt
      541 cgttccgttc cattcccatg tctcatcttt atatgaactg ctgtaaaccc ctccgaacat
      601 aaggatattc ccgcgagcac tgtcgtaagc catcacatgg ttgtatcgtg ccgatggctt
      661 gttcagggag tctttctgtg tccagttcga tccatcccat tcccatgttc cttccgtaac
      721 cgaggagcct ccgaacagga cggttttctc ccgtacgcgg tcataagcca ttgcatgacc
      781 ctttcgtgcc gaaggacagg tatcggcggt gcaaaggcaa gcaccggtac agatcgtgct
      841 ggccggccct ttgacctgcg cccagttcgt tccattccat tcccatgtgt cctggttggc
      901 atgggaagca ttgtcatagt aacttgcccc gccgaacagg atcaccttcc cgcgggcact
      961 atcgtaggcc atcgtatgag cggttcgagc cgtgggcttg ttcgtcgaag tcctctgtgt
     1021 ccagtttgcc ccgtcccatt cccatgtgtc ttgataatcg tgagaatcgc cgtcatagta
     1081 atcccccccg ccgaacagga ccactttccc gcgtacgctg tcgtatgtca tcgcgtggct
     1141 attccgtgcc gatggcttat ttgtcggata cctctgtgtc cagttcgttc catcccattc
     1201 ccatgtctcg ttgttgctcg attcaatagc cccgccgaac aggatcactt ttccccgcgc
     1261 gctatcgtaa gccatcgcat gaccggcgcg tgcggacggt ctggtcagcg ggttcaattt
     1321 ggtccagcta gccccgtccc attcccatgt ctcattggtc gatgcggccc cgttaaatcc
     1381 tccgaacagc aacaccttgc cgcgcacact gtcataggcc attgcggacc cttcccggcc
     1441 gaccggaact tcgttgtcgt ctttccaccg ctcccatgaa ccatcccctg atcgggcgat
     1501 cttttcctgt cctcccccgg cgggctcttg taaaaaggtc cccttttgct caaggattcg
     1561 atctatcatg ctgatcgtta tgaaggtatg cgggttctcc agagcgcttc ccggcacctt
     1621 tccccccatc gtggcgtgcc actcgatctc ggtgacgagg gcgccgttgc cggccgcctt
     1681 gaggccggac tttttcaccg ggttcaggta tatctccggc aggtcgccgc cggtgagcgt
     1741 cggtatgttg aacgcgccgc cggtcacgct ggtctgcccg atgatggagc cctgttcgtt
     1801 gtaggcgatg atcgtggcga tatcggcgct tccgaccgca ccggactccc ccgccacgct
     1861 gaacttcggc ttgcccgcgg tgtcgtcggt cccccacggt tttctcgtgt aaagcacctt
     1921 cttcatatcg acgagcgccg cgtccggcag tgtcttatcg aggaacatct tccagtccac
     1981 atcccgtgtc gcttcgttcc cgagcaggtc gcgccaggtc accgtgaagg catgctcgcc
     2041 cgcggtgatg gagtcgtcca gttccttctc gaactgcgcg aagttgccgg tcacctcgac
     2101 cgccgcgccg aagtcgagtc ccgcgaccgc cggggtcgcc gggtcgagtt cctcgtcggc
     2161 gtagaggtta agttgggccg tcaccacctc atcggtgcgc gggtcggtga gactgaagga
     2221 gagaaccttg ttctcagcgt cgcgcgccgg ggcgaagtcg gggatccgtt ggagaagggc
     2281 gctcgtgagt atcggcccgc tgaagtcgaa gatcgcgcgg caggtatctt tctcgatacc
     2341 cccgatggtc gggagcaccg ttcccgaata gagattcccg gccgagtcga tcgcctcgat
     2401 aaggacccgt ttgttccctt cggggttggt gttcgagtgg atgaccgtct gtgcccaaca
     2461 gaaggcgttc ggtgtttcgc actcttcaac catgctttcc gatcccagtg taagcagcgg
     2521 cgcgaggccg gtgctgtcca gattgtcctc gaccgttatg tcgagcgtga cctcgaccac
     2581 atcgccctct ctggcggcgg cgatggagag aggttctccc gagaaggtgt cggtgtagaa
     2641 gaggtcccgt cgcgtccgta ccgaacagcc ggtgacggtc acttcgggag gcgtcttgtc
     2701 tatcgtgagg atatccgcca gttcgtccgc cagttcgttg ccgacggcgt cagtcgcctc
     2761 caccgtcagc acatagtcgc cttcggcatc ctcgccaccg gagcctatca catattcgaa
     2821 cacgagtacc tgctcgttgc tgatatcttc gttcagcgaa agggaaaagg cggtctccgt
     2881 cagggtggcg tgccgcgccg acacctgtac cccctgcacc ggttcgtgga aactgaggct
     2941 cagggtgacc ggccgtccgc cgttcgcctt ctgcggatcg agtacccggt tcacgataaa
     3001 gggaggtttg ttgtcgatga acagttcaaa ggaggccggt ttcgattcat agccgacgct
     3061 gttcatcgtt ttgagataga cggtccgctg tgcgcggtcg gtcagatcgg tctcgatcgt
     3121 gcgaccgtag ttgaggtccc acgccgtttc gtaccagtcg gtgccgtagc cctcctcctc
     3181 agccagagag gtgagttcgg taagggcgag ttccttggtg cttgccgtct tgaagccggt
     3241 ctcgttgccg atgaccgcct gcgaggcgct ttccgccttg aagcggagca ggaccgccgg
     3301 ttccttgacg accgcgccga gtgaaagttc ggtagcgccg tcggcgctga atatgcgcag
     3361 agagggggtg ccgccttcgg tgaccgcggg ccctttctgc atgacgcagg cgttgttgac
     3421 atcggtcact ttgccgcggg cgagggagga ctcctgtgat atgcagaagt aggcaaggtt
     3481 gtcgggtcgc gcctcggggg cgacctcctt gtgggccggg tcttccgcgg
//
LOCUS       DAOQUE010000290         3528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_30275_length_3528_cov_5.725310, whole genome shotgun sequence.
ACCESSION   DAOQUE010000290 DAOQUE010000000
VERSION     DAOQUE010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30275_length_3528_cov_5.725310"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3394)
                     /locus_tag="PKH10_08990"
     CDS             complement(<1..3394)
                     /locus_tag="PKH10_08990"
                     /inference="COORDINATES: protein motif:HMM:NF025223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28297.1"
                     /translation="MTHGDHTIKAIATDTAGNTTATPIVKTYTVDAIYPEATVNGFPA
                     TTEHFLRTTAVDFTVTSNDSNDPLTIEVLRSDGNWVPCTSGACRYTTADGADNMDTQE
                     TVTIRYADVAGNSVNQTAGTVYVDRTAPTITLSLNKTSFNAADTIQATVTTTEELTGE
                     PIVEIPSVPMTLGDPTQISTFSWLYTLDPHLLADNNYTMNAAGTDLVAYDDQDTKTFS
                     VDTSIPDLEEGAYGIDRAKAGIGITYTVDFETTEPVEGTDGVPTVTANEIILSCAKNG
                     SDTVFRCTRTVGAEETDATVYGIRTTLTDAAGNSRTVTIGSVMADKTLPDAVAGTVVL
                     SIDAGLDCALTQEQVTTLADGSTAHLSVQVSEKLANDYEPVLTAKRTDAPDRAFHLDA
                     QNGLTYYFSHLYDEDDPEPVMIGEYTLELTLKDEAGNENTIENLVSDKAFVTAENTDP
                     ELNINQDLVSYNRSPWGRAIEMELKDEADNVVYAIPANDYFELGPKDIYAAVNRLPAE
                     TFVLNYGPHTETPVMLNVWAKDGTQLRMAHPSPNPDGTWPRSRVWSLDRPVISVSALD
                     SACRETPRVKIRDNNWIATMNKKVPGSTFENPHIFTTIGSFKETLEQYENEKTEPPLT
                     DNKGLTTFNSYLSKKGTYNNRWTQLSSSASGGSPLKRQNAMMVYDSARAKIVLFGGVQ
                     TSNFTPLNDTWEWDGASWKELRPSISPSAREAGAFVYDKARGKVLLFGGGGWYGVGDF
                     DDTWEWDGTNWVKRAPVVSPAARSGHGMVFDSVRGKTILFGGFTGNYPDFSYYDDTWE
                     WDGTNWTEKWPNNSPAGCFAGNAMAYDRSHHRIVLFGCGKIDGNNNDTWEWDGENWEK
                     KSPVEPPHLRHGHAIEYDIVHERTVLFGGWGVVGGVMSQLNDSWEWDGNNWTQTSPTR
                     NPSARSFHAMAYDNIRERMVLFGGQGADTAFNDIWEWDGIDWTQKDGTGQTSWPSTRR
                     RTALAYDADRGKTVLFGGYDSGKTCSSQGDSTYCGDMWEWDGRDWTKRFSSGGSYGHV
                     MAYDTVKRKVFGFGGGIYGDSNTSWEWDGTSWSNLDPAHKPPTREDFAMAYDSIRGKM
                     VLFGGYDSSKECTAGLSTHCNDTWEWDGISWAQSDPPTKPPAR"
BASE COUNT          691 a          999 c          926 g          912 t
ORIGIN      
        1 cacgcgccgg cggcttagtc ggcggatcgc tttgcgccca actgatacca tcccactccc
       61 aagtgtcgtt gcagtgagta gataatcccg cagtacattc tttggaagaa tcgtaacctc
      121 caaacaatac catcttgccc cggatactat cataggccat tgcgaaatct tctcgtgtcg
      181 gcggtttgtg tgccgggtcc aaattgctcc aactagtgcc gtcccactcc caagaggtat
      241 tgctgtcccc gtatatcccg ccgccaaaac cgaaaacctt tcttttcacg gtatcgtacg
      301 ccatgacatg accgtaactt cctccggaag agaagcgttt tgtccaatca cgaccatccc
      361 attcccacat atcaccacaa tacgttgaat cgccttgaga agaacatgtt ttaccggaat
      421 catagccccc aaacagaacg gtctttcccc ggtcggcatc ataggcgaga gcagtcctgc
      481 gccgagtgct gggccaagag gtttgccctg ttccatcctt ctgtgtccag tcaataccat
      541 cccactccca aatatcgtta aaagcagtgt ctgctccctg cccaccgaaa agcaccattc
      601 tctcgcgaat gttgtcatat gccatggcat gaaatgaacg tgcggatgga tttctcgtcg
      661 gtgatgtttg tgtccaatta ttaccatccc attcccagct atcatttaac tgactcatca
      721 cgccaccaac aactccccat ccaccgaaaa ggacggtcct ttcatgaaca atatcatatt
      781 ctatggcatg gccgtgacga agatgaggag gctctacagg ggattttttt tcccaattct
      841 caccatccca ttcccatgtg tcattattgt ttccatcaat tttgccacaa ccgaagagga
      901 cgatccgatg atggctacgg tcataggcca ttgcatttcc agcaaagcac cccgcgggac
      961 tgttgttggg ccacttttct gtccagttag tgccatccca ttcccatgtg tcgtcataat
     1021 aactgaaatc gggataattc cctgtgaatc caccaaacag tattgtttta cctctcacgc
     1081 tgtcaaaaac cattccatgt cccgaacggg ctgccgggct gacaacgggc gctcttttca
     1141 cccagttcgt gccgtcccac tcccaagtat cgtcaaaatc gcccactccg taccatccgc
     1201 caccaccgaa aagcaaaacc ttccccctcg ccttgtcgta cacaaaagcg ccggcttccc
     1261 gtgctgaagg acttatggat ggccgcagtt ctttccaaga agctccgtcc cactcccaag
     1321 tatcgttaag gggtgtaaaa tttgaagtct gaacgccgcc gaaaagcaca atttttgctc
     1381 tcgcgctatc gtaaaccatc atggcgtttt gtcgttttaa aggacttccc ccggaagccg
     1441 aagaagacag ttgtgtccac cgattattgt aggtcccctt cttggacaaa tagctattga
     1501 atgtcgtcag tcccttgttg tcggtcaggg gaggttctgt tttttcattt tcgtattgtt
     1561 ccaaggtctc tttgaaagag cctattgtcg taaagatgtg ggggttctca aatgtacttc
     1621 cgggcacctt cttgttcatg gtcgctatcc aattgttgtc ccggatcttt actctggggg
     1681 tttcccggca agccgaatca agggcggaaa cactgatgac cggtcggtcg aggctccata
     1741 ccctgctccg cggccatgtg ccgtctggat ttggcgaggg atgcgccatg cgcagttggg
     1801 tgccatcttt cgcccataca ttaagcatga ccggagtttc cgtatggggc ccgtaattta
     1861 aaacaaatgt ttcggccggc aggcgattga cagcggcata gatatctttc ggtcctaatt
     1921 cgaagtaatc attcgcagga atggcgtaca ccacattgtc cgcttcatct ttcagctcca
     1981 tttcgattgc cctgccccac ggtgaacggt tgtaactgac aagatcctga ttgatgttca
     2041 gctctggatc ggtgttttct gccgttacaa acgctttatc actcacaagg ttctctatgg
     2101 tgttttcgtt ccccgcctcg tccttgagcg tcagttctag cgtgtattct cctatcatca
     2161 cgggttccgg atcatcctcg tcatacaggt ggctgaagta gtaggtcaac ccgttctgcg
     2221 catcgaggtg gaaggcgcgg tcaggggcat cggtcctttt cgccgtcagg actggttcgt
     2281 agtcattcgc cagtttttcg ctcacctgta ccgagaggtg ggcggtcgag ccgtcggcga
     2341 gcgtcgttac ctgctcttgc gtcagggcgc aatcgagacc agcgtctatc gagaggacca
     2401 ccgtgccggc cacggcgtcg gggagtgtct tgtcggccat caccgacccg atcgtcacgg
     2461 tgcggctgtt tccggccgcg tcggtgagtg tcgttctgat gccgtaaacg gtggcgtcgg
     2521 tttcctcggc ccccacggtg cgggtgcacc ggaacaccgt atcgctcccg ttcttcgcgc
     2581 aggaaaggat gatctcgttc gccgtcaccg tcgggacacc gtcggtcccc tctaccggct
     2641 cggtcgtctc gaagtcgacc gtgtaggtga ttccgatacc cgccttggcg cgatcaatgc
     2701 catacgcccc ttcttcaagg tccgggatag aggtgtccac ggagaaggtc ttggtgtcct
     2761 ggtcatcata ggcgaccagg tcggtgccgg ctgcattcat cgtgtagttg ttgtcggcga
     2821 gtagatgcgg atcgagtgtg tagagccacg agaaggtcga tatctgcgtc ggatcgccga
     2881 gcgtcatcgg gaccgatggt atctcgacga tcggctctcc ggtcagttcc tccgtcgtcg
     2941 ttaccgtcgc ctgtatcgtg tcggccgcat tgaatgacgt tttgttgaga ctcagcgtga
     3001 tggtcggcgc cgtgcggtcc acatagaccg tgcctgcggt ctgattgacg ctgttgcccg
     3061 cgacatcggc gtaccggatg gtcaccgttt cctgtgtgtc catgttgtct gcgccatccg
     3121 cagtggtgta cctgcatgcg ccgctcgtac aggggaccca gttgccgtcc gaccgcagta
     3181 cctctatcgt gaggggatcg ttgctgtcgt tgctggtcac cgtgaagtca acggcggtag
     3241 ttctgagaaa gtgctcggtg gtcgccggga aaccgtttac cgtagcttcc ggatagatcg
     3301 cgtcgacggt gtaggtcttg acgatggggg tggcggtggt gttgccggcg gtgtcggtgg
     3361 cgatcgcttt gatggtgtga tcgccgtggg tcaacgccgg ggcgtatatc cacgagttcc
     3421 cttccggtgt cgggtcgtcg agaggtacct cgtcaagggt cacgaccggg gcaccgtcaa
     3481 gttcttctat gacgctgacg gtgatgatga gttggtcaag ggcattgt
//
LOCUS       DAOQUE010000291         3518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_30410_length_3518_cov_5.211377, whole genome shotgun sequence.
ACCESSION   DAOQUE010000291 DAOQUE010000000
VERSION     DAOQUE010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30410_length_3518_cov_5.211377"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="PKH10_08995"
     CDS             <1..1081
                     /locus_tag="PKH10_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28298.1"
                     /translation="ATVQHAQSSVELGCADSTECIVEVQKKLAVDTLIAGKITKMGDE
                     YILSLNTVDVNNSIVGKRTNVQGVDLNDIKAKIKEAVDKLLGLAKEKPMFTLKEGEQL
                     KLAVMPLSARGVEIATADALTSILSSQLNQIKGISVISQDDIKAMLAKVSMDSAVECT
                     DSMQCVVEIGASLGLSKLVTGSVGKVKDTYVISIQLIDTRRAEVENRVLESFAGDAEE
                     LKNAVKLAAYQLAGIDYTSRPGSIAITFNVPEGELQFGPNKEKLINSQYTAENLVPGR
                     YNLRVLADQDDYFPFQTDIYVAPGTANVRTLTVLERPTPWYKTWWFWTITGTVIAGAA
                     TATGVVLGLKKTELGTGKVQIEMAR"
     gene            complement(1151..1651)
                     /locus_tag="PKH10_09000"
     CDS             complement(1151..1651)
                     /locus_tag="PKH10_09000"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HNT28299.1"
                     /translation="MRKLLFFAILMMSALALAQCPNHGKGGPGGPEGGPGKHDKGAHM
                     MKMHDEMLEAIGVPEAQRAAFRASAHENHKKMMDIQFKIKEERIAMKAEAEKPSPDKA
                     KLQKSIQKIADLRRDQTLLMENHKLEMLFKLTPDQRKKALDFMQDRKKMMMERFGGPG
                     PDGDDD"
     gene            complement(1673..1921)
                     /locus_tag="PKH10_09005"
     CDS             complement(1673..1921)
                     /locus_tag="PKH10_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28300.1"
                     /translation="MRNEQERWETMLSRVKERGGRIRRRRQWAAATTAALALMLVVTV
                     QFGLTRPAPEPQSFALNEVEMEVVLLEHDIVMDEMGLY"
     gene            complement(1914..2465)
                     /locus_tag="PKH10_09010"
     CDS             complement(1914..2465)
                     /locus_tag="PKH10_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT28301.1"
                     /translation="MFGFLESRKERDRKFDEIFWEKRHFIESIAKRYARTPDLIDDLV
                     QEIFLKIYLQLPKVLAADNRDGYLRRLAVNSVVDYFRANRDKMREVTLSSELEEVLPA
                     TGDGHEAATLAAQRAERFMGMVQALPEKRRNVMTLRIVDELPFRDIGETLGISEVSAR
                     NLFSIGMKELKMKILRQQEAGHA"
     gene            2622..>3518
                     /locus_tag="PKH10_09015"
     CDS             2622..>3518
                     /locus_tag="PKH10_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT28302.1"
                     /translation="MFGMELGKRYEHQGIESKWYKEWEERGYFAARPESDKPPFCIVI
                     PPPNVTGSLHMGHAVTVTIQDVLCRYKRMAGFDVLWLPGCDHAGIATQMVVERELAKE
                     GKSRHELGREKFLEKVWDWKRQYGNRISEQLRHLGSSLDWSRERFTMDDGLSRAVIEV
                     FVRLYEEGLIYRDTYIINWCPRCRTALSDLEVDHEEKDGALWYIKYPVKGTGEFLTVA
                     TTRPETMLGDTAVAVHPDDPRFRHLIGKSVILPLMEREIPIIGDAELVDIEFSTSAMK
                     VTPTHDFNDFATGKRHNLAKINV"
BASE COUNT          716 a         1075 c         1045 g          682 t
ORIGIN      
        1 cgcgacggtc cagcatgctc aatcgagcgt cgagctgggg tgcgccgatt cgaccgaatg
       61 catcgtcgag gtgcagaaga aactggcggt cgacaccctg atcgccggaa agataacgaa
      121 gatgggcgac gaatacatcc tttcgctcaa caccgtcgat gtcaacaact caatcgtcgg
      181 caagcggacc aatgtgcagg gcgtcgacct caatgacatc aaggcgaaga taaaagaggc
      241 ggtcgataaa ctgctcggtc tcgcaaagga aaagccgatg ttcacgctca aggagggcga
      301 acagttgaag ctcgccgtca tgccgctttc ggcgcgtgga gtcgagatcg cgacggccga
      361 cgctctcact tcgatacttt cttcgcaact taaccagata aagggcatca gcgtcatcag
      421 tcaggatgac atcaaggcga tgctcgccaa ggtgtctatg gactcggcgg tcgaatgcac
      481 cgacagcatg cagtgcgtgg tcgagatcgg tgcgtcgctc ggcctctcca agttggtgac
      541 cggttcggtc ggcaaggtca aggacaccta tgtcatctcg atacaactga tagacacgcg
      601 ccgtgccgag gtcgagaacc gcgtgctgga aagtttcgcg ggtgacgccg aggaactaaa
      661 gaacgccgtg aaactcgccg cctaccaact cgccggcatc gactacacga gcagaccggg
      721 gagcatcgcg ataaccttca atgtgccgga gggcgagttg cagttcggcc cgaacaagga
      781 gaaactgatc aacagccagt acaccgctga gaacctcgtc ccgggccgct acaacctgcg
      841 ggtgcttgct gatcaggacg actacttccc gttccagacc gatatctatg tcgcgccggg
      901 gaccgcgaat gtccggacgc tcaccgtcct ggagcggccg acgccgtggt acaagacctg
      961 gtggttctgg accatcaccg gcaccgtcat cgccggcgcg gcgaccgcca ccggcgtcgt
     1021 gcttggactc aagaagaccg aactgggcac cggcaaggtc cagatagaga tggcccgctg
     1081 aacggcgacg gcccgccgcg ccgccggagc ggagcgcacg ggccgcgtat cgtgggttcg
     1141 agaggggatg ctagtcgtcg tcaccgtcgg ggccggggcc gccgaatctt tccatcatca
     1201 tcttcttgcg atcctgcatg aaatcgagcg ccttcttgcg ctggtccggg gtgagtttga
     1261 atagcatctc gagtttgtga ttctccatga ggagcgtctg gtcgcgccgc aggtcggcga
     1321 tcttctggat cgacttctgg agtttcgcct tgtcgggact cggcttctcg gcctcggcct
     1381 tcatcgcgat gcgttcctcc ttgatcttga actggatgtc catcatcttc ttgtgattct
     1441 cgtgcgccga agcgcggaat gccgcccgct gcgcctcggg gacgccgatc gcctcaagca
     1501 tctcgtcgtg catcttcatc atgtgggccc ccttgtcgtg cttgccgggt cctccttcgg
     1561 ggccgccggg ccctcctttg ccgtggttgg ggcactgggc gagcgcaagt gcgctcatca
     1621 tcaggatcgc gaagaacagt aactttctca taggttcctc cctatgcggt ggttaataaa
     1681 ggcccatttc atccataacg atatcgtgct cgagcaacac cacttccatt tcgacctcgt
     1741 tgagcgcgaa actctgcggt tcgggcgcag ggcgcgtcag gccgaactgc accgtgacca
     1801 cgagcatgag cgccagcgcc gcggtggtcg ccgccgccca ctgacgccgc cgccggatgc
     1861 ggccgccacg ctccttgacc cgggagagca tcgtctccca ccgttcctgt tcgttacgca
     1921 tggcctgcct cctgctgtcg cagtatcttc atcttaagtt ctttcatccc tatcgaaaag
     1981 aggttccgcg ccgacacctc ggagatgccg agcgtctccc cgatgtcgcg gaacggcagt
     2041 tcatcgacga tccgcagcgt catgacattg cgccgttttt cggggagcgc ctgcaccatc
     2101 cccatgaacc gctcggcccg ttgcgccgcg agcgtcgccg cctcgtgacc gtcgcccgtc
     2161 gccgggagta cctcctccaa ttcggacgag agcgtcacct cgcgcatctt gtcacggttg
     2221 gcgcggaaat aatcgacgac gctgttgacc gccagccgcc gcagatagcc gtcgcggttg
     2281 tcggcggcga gcaccttggg gagttgcaga tatatcttga ggaatatctc ctgcacgaga
     2341 tcgtcgatga ggtccggggt ccgggcgtag cgcttggcga tgctctctat gaaatggcgc
     2401 ttctcccaga agatctcgtc gaacttccgg tcgcgctctt tcctgctttc gaggaacccg
     2461 aacatcgtca atgcctcctc agcacacacc cactataacg cggtcgaggg aaaaatggta
     2521 aaaaaacaga tcggtctcgc gcgcgaacca caaagggtgg aataacttta cttttcgccg
     2581 accgtaggta aaagaaacga gcgttatttt tcctgacggg gatattcggt atggaactcg
     2641 gaaagcgcta cgaacatcaa ggcattgagt cgaaatggta taaggagtgg gaagagcgcg
     2701 gctacttcgc cgcccgccca gagagcgata agccgccgtt ctgcatagtc atcccgccgc
     2761 ccaatgtcac cggctcgctc cacatgggac atgccgtgac ggtcaccatt caggatgtcc
     2821 tgtgccgtta caaacggatg gcgggattcg atgtgctgtg gctccccggt tgcgatcacg
     2881 ccggcatcgc cacccagatg gtggtcgagc gcgaactggc gaaagagggg aagagccgtc
     2941 acgaactggg gcgcgagaag ttcctcgaaa aggtgtggga ctggaagcgg cagtacggca
     3001 accggatatc ggaacagttg cgccatctcg gcagttcgct cgactggagc cgcgaacggt
     3061 tcacgatgga cgacggacta tcgcgcgccg tcatcgaggt cttcgtgcgg ctgtacgagg
     3121 aagggctcat ctaccgcgac acctacatca tcaactggtg cccgcgctgt cgcaccgcgc
     3181 tgtcggacct tgaggtcgat catgaggaaa aggacggtgc gctctggtat atcaagtatc
     3241 cggtgaaagg aaccggcgag ttccttaccg tcgcgacgac ccgccccgag acgatgctcg
     3301 gcgacacggc cgtggcggtc catcccgacg acccgcgctt ccgccatctc atcggcaaga
     3361 gcgtgatact gccgctcatg gagcgcgaga taccgatcat cggcgacgcc gaactggtcg
     3421 atatcgagtt cagcaccagc gcaatgaagg tgaccccgac ccacgacttc aacgacttcg
     3481 cgaccggcaa acgccacaac ctcgccaaga taaatgtc
//
LOCUS       DAOQUE010000292         3506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_30555_length_3506_cov_4.293828, whole genome shotgun sequence.
ACCESSION   DAOQUE010000292 DAOQUE010000000
VERSION     DAOQUE010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30555_length_3506_cov_4.293828"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PKH10_09020"
     CDS             <1..480
                     /locus_tag="PKH10_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28303.1"
                     /translation="ETVPVVNPKAVASPETLKETPKEEAPLPPVIEEPPSKRPYLWYG
                     VGLLAFGVASAGAGAGLMVQADLFYKKYNDIVSTENLIEMRERLSEAAFVQYVRDAKD
                     KYQTPGDTYSDTGIALISVGGAAVVTGLVLTLITEEAPTVTMNFDEKGFSIGYAFKF"
     gene            499..2685
                     /locus_tag="PKH10_09025"
     CDS             499..2685
                     /locus_tag="PKH10_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HNT28304.1"
                     /translation="MRCLCLVPLVLLAAVGCTKPNDIDWNKVFGDKDSAAAADDDSAV
                     PDDLQPDETVPDEATDKTMPDETGEEDLPDEVEDAVTEEKPDGTDIEETEEDAIEPEE
                     DAQKDAETDVDDEYIEPDDWEDDGEEVGDDASGPDDDTLDEDAVDAPSDGEASVDLDN
                     TLPDNDLVPFCGDGKHEIPNGLVLYLRMDEPASFGAAYDSSVSPLTGHGYFTQGALLG
                     NIGIRGTALSFNKNSDFQVPDAAKLDLAGPMSLSAWINLPDNGSGNSTPYLVIGKGSG
                     RQAAFYVWREADRTINFGFHTWELTGTGCGARSVTTPMDDGAWHHITGTYDGTYVRMY
                     VDGVLETTSDPCLDVPMPNDHPVTIGHVPWTLIDYPDTYTGTGYIDEVRIYDRALSLS
                     EIAALKNTHTEYCDDGDETITGDGCTNCQVDAGHICYGSPSTCILEPSRTCTHSVNIC
                     DSAEDYYDASYSEYYYRSWVGTTLQITTNSSVKWTSPSFSTYQWYEYSSGAKCWGTWY
                     NYTFDHGQTVEATYTAKGAYDPHECYFQINDAPNGGGVVIATTAATVSGQWPANYSYT
                     GTCFASSPSPFCGDGATQEGLEECDDNNTSNCDLCSSICKNKPANYCGDGYICGSEKC
                     EGEDLNGKTCLTQVPGAEWGKLSCNGACDFVTTACSKNYTCAGKPSTGTLWNTVSSYV
                     QTWNGSAWSPVDDPTTEYNPTASSTSCRYKCDAANGYSWSGSSCVK"
     gene            2740..>3506
                     /locus_tag="PKH10_09030"
     CDS             2740..>3506
                     /locus_tag="PKH10_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNT28305.1"
                     /translation="MRSFTVSQILLFVIALFLGCTKPNTIDWDKVFGDRDASPDNMVT
                     LDEEEWPDEESPAEETTPPDNDTSEDDEYVAEHDMPEGDDVDVATDHVLPTEEDILLS
                     DDAVEVEDTVTDPDFDLVVDETSPWITSTEPESDATDVLVDVIITITFSEALDGTKVT
                     GENILLERSGESVLVAITYRSDDHSVLITPTTPLDHGVAYTVTVRTGITDLAGNAFEA
                     EHVFGFVTTLCGNGVPDLGEQCDDGNTDPCDGCLNNCT"
BASE COUNT          868 a          869 c         1000 g          769 t
ORIGIN      
        1 gaaacagtgc cggtggtgaa ccccaaggcg gtggcttctc cggaaacttt aaaggaaacc
       61 cccaaagagg aggcgccact acctccggtg attgaagagc ctccgtcaaa gcgcccttat
      121 ctctggtatg gcgtcgggct tctggccttt ggtgtagcga gcgcaggggc tggcgccgga
      181 ctcatggtgc aagccgatct cttttacaaa aaatacaatg acatagtaag tacggagaac
      241 ctgatagaga tgagagagcg cctttcagaa gccgctttcg ttcagtatgt ccgtgacgcg
      301 aaagacaaat accagacgcc gggtgatact tacagcgata ccggtatcgc tttgatctcg
      361 gtcggtggtg cggcggtcgt gaccgggctg gtactcacgc tcatcactga ggaagctccg
      421 accgtcacca tgaatttcga tgaaaaaggc ttttcgatag ggtacgcatt caagttttaa
      481 atgacatcga ggtgcccgat gcgctgtctt tgccttgttc ccttggtgct ccttgccgct
      541 gtcggctgta ccaagccgaa cgatatagac tggaataagg tgtttgggga taaggacagc
      601 gccgccgccg ctgacgacga cagcgcggtt cccgacgacc tccagccgga cgaaacggta
      661 cccgacgagg caacggacaa gacgatgccc gatgaaacag gggaggaaga ccttcccgac
      721 gaagtagagg acgccgtcac cgaggaaaag ccggatggca cggatatcga agagacggag
      781 gaagacgcga tagagccgga ggaagatgcg cagaaagatg cggagacgga tgtcgatgat
      841 gagtacatcg agcccgatga ttgggaagac gacggagaag aagtagggga cgatgcttcc
      901 gggccggacg atgataccct cgatgaagat gcggttgatg ctccatccga tggggaggca
      961 tccgtagacc tagataatac gctccccgac aacgatctcg tgccgttctg cggtgacggc
     1021 aagcatgaaa ttcctaacgg tcttgtacta tacctccgca tggacgaacc cgcttccttc
     1081 ggggcggcat atgattcatc cgtgagcccg ctgaccggac acggatactt tactcaaggt
     1141 gcgttgttag gtaatattgg tatccgagga acggcattgt cctttaataa aaacagcgac
     1201 tttcaagtgc ccgatgccgc aaaactcgat cttgccggcc ccatgagcct atcggcgtgg
     1261 attaatctgc ccgacaacgg atccggtaat tcgacacctt atttggtcat aggcaaaggt
     1321 tccgggagac aggccgcctt ttatgtctgg cgagaggccg accgaacgat caactttgga
     1381 ttccatacat gggaacttac cgggaccggc tgtggtgctc gatcggtcac gacacctatg
     1441 gatgacggtg cgtggcatca tatcacgggg acctatgatg gcacctatgt aaggatgtat
     1501 gtagatggcg tcttggaaac cacctccgat ccgtgtcttg atgtcccgat gcccaacgat
     1561 catccagtga ccatcgggca tgtgccgtgg accttgatcg attatcccga tacctacact
     1621 ggtaccggtt atatcgacga ggtacggata tatgatcgtg ccctctcgtt gagtgagata
     1681 gcggcactga aaaacaccca caccgaatac tgcgacgacg gcgacgagac catcaccggc
     1741 gacggttgca ccaactgtca ggtcgatgcc ggtcatatat gctatggttc accgtcgaca
     1801 tgcatcctcg agccgtcgcg gacctgcacc cattcagtga atatctgtga cagcgccgaa
     1861 gactattatg atgcctctta tagcgagtac tattatcgaa gttgggtcgg tactacgcta
     1921 cagataacaa ccaacagttc ggtgaaatgg acatcgccgt ccttctctac ataccagtgg
     1981 tatgaatact cgtctggcgc gaaatgctgg ggtacttggt acaactacac ttttgatcat
     2041 ggtcaaacgg tagaggctac ctatacggcc aagggagcct atgacccgca tgagtgttat
     2101 ttccaaataa acgatgcccc gaatggtggc ggcgtcgtca tcgccaccac cgcggcgacc
     2161 gtcagcggcc agtggccggc gaactattcc tacacaggga cctgttttgc ttcctctcct
     2221 tctcctttct gcggagatgg cgctacgcaa gagggacttg aagaatgtga cgataacaac
     2281 acgagtaact gtgatctttg ttcttccatt tgcaaaaata agccggctaa ttactgcgga
     2341 gacggttata tctgtggtag cgaaaaatgt gaaggcgagg atcttaacgg gaaaacctgc
     2401 ttgacgcagg ttcccggtgc tgaatggggc aaactttcct gtaacggcgc ctgtgacttt
     2461 gtcaccaccg catgttcaaa aaattatact tgtgccggaa aaccttcgac agggacgctc
     2521 tggaataccg tttcgagtta tgtgcagaca tggaacggtt cagcatggag cccggtcgat
     2581 gatccgacga ccgagtataa cccgacagcc agcagtactt cctgccgata caaatgcgat
     2641 gccgcgaatg gctattcatg gagcggtagt tcatgtgtaa aatgatattc gcgatgaaga
     2701 tcgttacctt gcgtgagaaa ggataagagg gaggatatta tgaggtcttt cacggtgtca
     2761 cagattcttt tgttcgtgat cgccctgttc ttgggttgca ccaaaccgaa cacgatagac
     2821 tgggataagg tgttcgggga cagggacgca tcgcccgata atatggtaac gctcgacgag
     2881 gaagaatggc ccgatgaaga atctcctgcg gaagaaacga caccgccgga taacgataca
     2941 tcggaagatg atgaatatgt ggccgagcat gacatgcccg aaggggatga tgtcgatgtg
     3001 gcgaccgacc atgttctgcc gacggaagaa gacatccttc tgtccgatga cgcggttgag
     3061 gtggaagata cggtgactga tcctgacttc gatctggtgg tggacgagac atctccgtgg
     3121 ataacgagca ccgagcccga gtcggatgcg acggatgtct tggtcgatgt gataataacg
     3181 ataaccttca gcgaagcgct cgacggcacc aaagtgactg gggagaacat ccttctagaa
     3241 agatcgggag aatcggtgct tgtcgcgata acctacaggt cggacgacca ttcggtattg
     3301 ataaccccga ccactccgct tgatcatggc gtcgcataca cggtgacggt gaggacgggt
     3361 atcaccgatc ttgcgggcaa cgcttttgag gcggagcacg tctttggttt tgtaacgacc
     3421 ctctgtggga atggtgtgcc ggatcttggc gaacaatgtg acgatggcaa cacggatcct
     3481 tgcgatgggt gcctgaataa ctgtac
//
LOCUS       DAOQUE010000293         3491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_30731_length_3491_cov_5.714494, whole genome shotgun sequence.
ACCESSION   DAOQUE010000293 DAOQUE010000000
VERSION     DAOQUE010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30731_length_3491_cov_5.714494"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..2086
                     /locus_tag="PKH10_09035"
     CDS             68..2086
                     /locus_tag="PKH10_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28306.1"
                     /translation="MKSMLFFFLALLMATPTVFARSKVLILPPAWESSSLKNFAQDMD
                     ENCDDLLPAIKNADGYCATTALPLKQKKTLSACKGKYACLKKKARALEEYDILVLSSL
                     SRKKGTPSLRVTFYNTRGDLLHREDLRGERNADPEETAALYLTALRNAHDLFARPANP
                     YEVKEALRRGFTFYKKGDIGQTLRFFEKAGDAVLLGLTGEVDALLARAEKSIERGEVR
                     DAIGDLQTALEKDEDIRKNGFKVISYIRETEKTKRSEFDQNDFYDDLLKIHSEFERRF
                     RDIEKTRAGEERAIADREKKAIEAVEYEIEQARKDIEKKEAATDKRRAVVDEEIRKLL
                     EKQETELRLLEKKKDIIRKRIDSLEQGRNEFIDKVERRADELRNTDLEKLQARFLKEQ
                     ENISKKITALESKDPPDEKAIGALEKKLAALEADLEKKQAALTEQYRRGADEHLKKYD
                     RKVDEVRNQLSKFEKNDASLVKGYQDRIAKKEAGFKQQVAQWHKEREKRERLIAALIK
                     NSEKRIAAAGREAQKQREQLKKRLNTEKEKVIAREKEMITKRYTARFKPVFNRVIERT
                     AATKKIGALLGTAYAMLGDRSLKDDDLPEARRHYHRALYHDPANKQAAKGIDLIRART
                     VKLYETAIAMSGDEPDRARQTLETIINSLKPTNPVYVRSFVALKELSK"
     gene            complement(2096..>3491)
                     /locus_tag="PKH10_09040"
     CDS             complement(2096..>3491)
                     /locus_tag="PKH10_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28307.1"
                     /translation="FPVLVIAGMFAATLSSAFGSILGAPRYLQALAIDGVVPRLFARG
                     HGPLNEPRIGLAATFFIAEAGILVGELNLVAPLITMFFLSSYGFACLACGIQTWSRIP
                     SFRPTFKTPAWVSFVGAVTCFGIMFKIDTLAMLAATAAMITIFILLQRRQMQTQPIDT
                     WRGFFTAAVHRGILYLDSRPPDGKNWRPNVIVFGDDPGKRRAIADMAGWLFHWRGVTT
                     YCHVVPGLLKEEAPRLRDMETQTREVIEQVAPETMVRLLVTEEPERGILDASQALGFP
                     GMTPNTVLMEWACPRMKHEPFTELVRGLLALDHNLLFLRHHPDRSFGMRKTIDIWWGG
                     RERNVELMLLLAHFLVSSDPWSGAKIRLCVLVDSDAAARKAEARLGAIIATARIEATP
                     YLIIRDERNTGELLGEVSERTDLVLAGLAGPPDEGPDGYVERMDTLLSGVGSALLVRA
                     SSQFDYRRLLLDEE"
BASE COUNT          885 a          975 c         1070 g          561 t
ORIGIN      
        1 ccggcgagcc gaattcgacg agatacgccg atcgaaagat gatagataat agataacgga
       61 gggatccatg aaaagtatgc tgttcttttt tcttgccctg ctcatggcga caccgaccgt
      121 gttcgcaagg agcaaggtgt tgatcctgcc cccggcctgg gaaagttctt cgctgaagaa
      181 cttcgcgcag gatatggacg agaattgcga cgatcttctt cccgccatca agaacgcaga
      241 cggttactgc gccaccaccg ctctcccgct gaaacagaag aagaccttga gtgcatgcaa
      301 gggaaagtac gcctgtctga aaaaaaaggc ccgggcgctt gaagaatatg acatcctcgt
      361 tctctcgtcg ctttcacgga aaaagggaac gccctccctg cgtgtcacct tttataatac
      421 ccgcggcgac ctgttgcacc gtgaggatct ccggggagag cggaacgccg accccgagga
      481 gaccgcggcg ctctatctga cggcgctccg gaacgcgcac gatctctttg cgcgaccggc
      541 gaacccctac gaggtgaagg aggcgctccg gcgcggtttc accttttaca aaaaagggga
      601 catcggccag accctgcgct ttttcgaaaa agcaggcgat gcggtattac tcggccttac
      661 gggcgaggtc gacgccctcc ttgcgcgcgc ggaaaagtcc attgaacggg gcgaggttcg
      721 tgacgccatc ggcgacctgc agaccgccct tgagaaagat gaggatatcc ggaaaaacgg
      781 cttcaaggtg atatcgtata tccgtgagac cgagaaaacg aaacggagcg agttcgatca
      841 gaacgatttt tacgacgatc tcctcaagat ccactcggag ttcgagcgcc ggttccgcga
      901 catcgaaaaa acacgggccg gcgaggaacg cgccatagcg gatcgggaaa aaaaggccat
      961 cgaggcggta gagtacgaga tagaacaggc gcgcaaagat atcgagaaaa aagaagccgc
     1021 cacggataag cggcgggcgg tcgtcgacga agagatacga aaactgctgg aaaaacagga
     1081 aacggaactt cgcctgctgg aaaagaaaaa ggatatcatt cgcaagcgca tcgattctct
     1141 ggaacaaggc cgtaacgaat tcatcgacaa ggtcgagcga cgcgccgacg aactgaggaa
     1201 tactgatctt gaaaagttgc aggcccgttt tctcaaggaa caggagaaca tatcgaaaaa
     1261 gataacggct ctggaaagca aggatcctcc cgacgaaaaa gcgatagggg cgcttgagaa
     1321 gaaactggcg gctctcgagg ccgacctgga gaaaaaacag gcggcgctga ccgaacagta
     1381 ccgccggggg gccgacgagc atctgaagaa atatgaccga aaggtcgatg aggttcgcaa
     1441 ccaactgtca aaatttgaga agaatgacgc ttctcttgta aaagggtacc aggaccggat
     1501 agcgaaaaaa gaggcgggct tcaagcaaca ggtcgctcaa tggcacaagg aacgggagaa
     1561 aagagagcgg cttatcgccg ccttgataaa gaacagcgaa aagcggatag ccgccgccgg
     1621 gcgggaggcg caaaaacaac gggaacaact gaaaaaacgg ctgaacaccg aaaaggagaa
     1681 ggtgatagca cgggaaaaag agatgataac caaacggtat accgcgcggt tcaagccggt
     1741 gttcaaccgc gtcatcgaac gcactgcggc cacgaaaaag ataggcgcgc tgctcggcac
     1801 ggcctacgcg atgctcggcg accggtcact caaggacgac gacctgcccg aagcacggcg
     1861 acattaccat cgggcgctct atcacgatcc cgccaacaaa caggcggcga aggggataga
     1921 cctcataagg gcgcggacgg tcaaattgta tgaaacggcc atagcgatga gcggtgacga
     1981 gcccgacagg gcaagacaaa cgctcgaaac gatcataaac agtctgaagc cgaccaaccc
     2041 ggtctatgtc cgttcgttcg tcgcgctgaa ggaattatcg aaataacctc tcccctcact
     2101 cttcgtcgag caggagtcgt cgataatcga actggctcga ggcgcgcacg agtagcgccg
     2161 atccgacacc tgagagcaat gtgtccatcc gctcgacata gccgtccggc ccctcgtcgg
     2221 gcggtccggc cagcccggca aggaccagat cggtccgttc ggagacctcc cccagcaatt
     2281 ctccggtgtt gcgctcatcg cggatgatga gatacggcgt cgcctcgatg cgtgccgtcg
     2341 cgatgatggc accgaggcgc gcttcggcct tgcgggcggc ggcatccgaa tcgaccagca
     2401 cacacagccg tatcttcgcc ccgctccacg ggtcggagga aacgaggaaa tgggccagaa
     2461 ggagcatcaa ttccacattg cgttcacgac cgccccacca gatgtctatc gttttacgca
     2521 taccgaagga acgatcgggg tgatgtcgca ggaacaacag gttatggtcg agcgccagca
     2581 gaccgcggac caattcggtg aacggctcgt gcttcatgcg ggggcaggcc cattccatca
     2641 gaactgtgtt cggtgtcatg ccggggaacc cgagcgcctg cgacgcatcg aggatgcccc
     2701 gttccggctc ctcggtcacc agtagccgca ccatcgtctc gggagccacc tgctcgatga
     2761 cctcgcgggt ctgtgtttcc atatcgcgca gtcgaggcgc ctcttccttc aaaaggccgg
     2821 ggacgacatg acagtaggtg gtgacgccgc gccagtggaa caaccaaccg gccatgtcgg
     2881 ctatcgcacg ccgcttgccc ggatcgtccc cgaacacgat gacgttgggg cgccagttct
     2941 taccgtcggg cgggcgactg tcgaggtaga ggatgccgcg gtggaccgcc gcggtgaaga
     3001 agccacgcca ggtgtcgatc ggctgggtct gcatctgccg gcgctgcagc aggatgaaga
     3061 tggtaatcat ggcggcggtg gcggcgagca ttgcgagtgt gtctatcttg aacatgatgc
     3121 cgaaacaggt gaccgccccg acgaaactga cccatgcagg cgtcttgaag gtgggacgga
     3181 aactggggat acgcgaccag gtctgaatgc cgcaggcgag acaggcgaac ccgtaactgg
     3241 agaggaagaa catcgtgatg agcggcgcga cgaggttcaa ttcgccgacg agtatgcccg
     3301 cttcggcgat gaaaaaggtc gccgcgagcc cgatacgcgg ctcgttgagc ggcccgtggc
     3361 cccgcgcgaa cagacgcggc accacgccgt cgatagcgag cgcctgcagg tagcgcggcg
     3421 cgccgaggat gctgccgaac gccgacgaga gggtcgcggc gaacatgccg gcgatcacca
     3481 gcaccgggaa t
//
LOCUS       DAOQUE010000294         3486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_30783_length_3486_cov_4.972603, whole genome shotgun sequence.
ACCESSION   DAOQUE010000294 DAOQUE010000000
VERSION     DAOQUE010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30783_length_3486_cov_4.972603"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PKH10_09045"
     CDS             <1..259
                     /locus_tag="PKH10_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT28308.1"
                     /translation="IQGLISAFDAVAHAAPEQKSALLASGIAVAMNTTGFGLGVAIPG
                     MALHMVLKALENKIFNEVDLYSVKLVNLLVSREKGTVSTEA"
     gene            278..829
                     /locus_tag="PKH10_09050"
     CDS             278..829
                     /locus_tag="PKH10_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNT28309.1"
                     /translation="MAKKRRPTEEVDTDLNLTPAMNLILILIPLLLTAMEQMKLAVIN
                     VATPQIGPASEVPPPDQAPDEKPLKLTIALTDRGMHLYARDQVIENKDDPLGPTIPKK
                     DGEEIIDGKPVPGKVYDYEKLTQMLADIKDENPVEENIIINAESHIKYRYIIQVMDAS
                     REKKDGDKKRSLFPNVVLAAGVA"
     gene            844..1437
                     /locus_tag="PKH10_09055"
     CDS             844..1437
                     /locus_tag="PKH10_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNT28310.1"
                     /translation="MAEEKNQNYEILSESYEQEMLAKKKAAKKGVRGETPAKLTMNSL
                     MDILTILLVFLLKSYSTDAVNVTPSSDLDIPKGKTMLTPEQTVVITVSKSAILVDNNM
                     ALGVKDGKVDGAQKRPPEDGYFIIPLNKMLVDAIEKQRRIAAVNPSAGKSLGIATIIM
                     DADTPFRLLTEVMYTASQAEFSKFKFLVIADATEGQG"
     gene            1503..2879
                     /locus_tag="PKH10_09060"
     CDS             1503..2879
                     /locus_tag="PKH10_09060"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNT28311.1"
                     /translation="MTQGSVADKVLRVGVIYNGKIIDEQVFRKPETIYIGDTAKAHFV
                     IPSSALPNRFPIFYYKSGKYELVVMENMTGKVSLKGKVIDIEEVVKKGMLKKRGSAYI
                     LPLSTDARGKIVVGSAIVLFQFITPPPKPPKLRLPRSIRGSWLQMIDWPLAAIQGSIF
                     IVGYAFIFWMYGLPDPAPKTLEQIDNRFARLIADVDQMKEKEKPVDETPTTDEGAAKT
                     AKKEEKKSDAGADEPVKKRKGPRDAATRAQEEAMRLSEMKKKVAGLGLMKMLGSTGGD
                     SSFGIVSDVLGEGAKDRDIDSALSGVKQIGVATDAGQRSRKGDAGATETAKIDDLKVA
                     DSAGKVKVGAVKEKEITGSTKVGAPEIEGTLDRGAINKIVKQNSPAIQRCYLNALKTN
                     PQLKGRIEVTWMINADGRAESVQITEDTVKDGGVTSCIKSVVSRWRFPKPEDPGIVAF
                     PFIFTPAE"
     gene            2907..>3486
                     /locus_tag="PKH10_09065"
     CDS             2907..>3486
                     /locus_tag="PKH10_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28312.1"
                     /translation="MKWLALTCAVAMLMIPLSTQAAQETASVSVDQMRSDIDGWARRV
                     TEIEAQIQKMNAQAVNEKDIKWKLCLDDILATIKGVSASIISARNRMEDMIRSEKKDA
                     AQIQFTLVKGLADAAERAFTESQGCPRQLTRVDTSSTIEKEEDKSITGTRGDKGGIGD
                     AMGQDFTEDFATERDPNDLGAEDPIDGAGTDTP"
BASE COUNT          876 a          895 c         1050 g          665 t
ORIGIN      
        1 gatccagggt cttatctccg cgttcgacgc cgttgcgcac gccgctcccg agcagaagtc
       61 ggccctgctg gcctccggta tcgccgtcgc gatgaacacg accggttttg gtcttggcgt
      121 cgctatcccc ggcatggcat tgcacatggt cctcaaggcg cttgagaaca agatcttcaa
      181 cgaggttgac ctctattcgg tcaaactggt gaatctgctt gtttctcgtg agaaggggac
      241 cgtctctacc gaggcttaaa ctcaggaggt tggaccgatg gctaagaaaa ggagaccgac
      301 ggaggaggtc gataccgacc tcaacctgac ccctgcgatg aacctcatcc tcatcctgat
      361 cccgttgctt ttgacggcga tggaacagat gaagttggcg gtcatcaatg tggcgacgcc
      421 gcagatcggc cccgcgagcg aggtgccgcc gcccgatcag gcgcccgacg agaaaccgct
      481 caaactgacc atcgcgctta ccgatcgcgg tatgcatctg tatgcgcggg atcaggtcat
      541 tgagaacaag gatgatccgc tgggtcccac catccccaag aaggatgggg aagagatcat
      601 cgacggcaaa ccggtgccgg gcaaggtgta cgattacgag aaattgacgc agatgctcgc
      661 cgacatcaag gatgagaacc cggtggagga gaacattatc atcaatgcgg agtcgcacat
      721 caagtatcgc tatatcatcc aagtgatgga cgcgtcccgc gaaaagaagg atggcgacaa
      781 gaaaaggtcg ctcttcccga atgtcgtatt ggccgctggc gtcgcgtagc aagggaggat
      841 acgatggctg aagagaaaaa tcagaattac gagatcctct cggaaagtta tgagcaggag
      901 atgcttgcca agaaaaaggc ggcaaagaaa ggcgttcgcg gagagactcc ggcgaaactc
      961 accatgaact cgctcatgga cattctcacc atcctgctcg tcttcctgtt gaagagttac
     1021 tccaccgacg cggtgaatgt cactccttcg agcgaccttg atatcccgaa aggcaagacc
     1081 atgctgacgc ctgaacagac cgttgtcatc acggtgtcga agtctgccat actggtggac
     1141 aacaacatgg cgcttggcgt caaggatggc aaggtggatg gcgcgcagaa acgccccccc
     1201 gaggacgggt atttcatcat cccgctgaac aagatgctgg tcgacgctat cgagaagcag
     1261 cggcgcatag cggcggtgaa cccgtcggcg ggcaagtcgt tgggcatcgc caccatcatc
     1321 atggatgccg atacgccttt ccggcttctt accgaagtga tgtacaccgc ttctcaggcg
     1381 gagttcagca aatttaagtt cttggtgatc gctgatgcta cggaagggca agggtagggt
     1441 ccacgaattg tttttgtatg cttcttccaa tgagtgagga gtatctctga cgagggggtc
     1501 acatgacaca gggttctgtt gccgataaag ttttacgcgt tggcgttatc tacaacggca
     1561 agataatcga tgagcaggtc ttccgtaaac cggagaccat ctacatcggc gataccgcca
     1621 aggcccattt tgtgatcccc tcttcggccc tgccgaaccg ttttcccatc ttctactaca
     1681 aatcgggaaa atatgagttg gtggtcatgg agaacatgac cggcaaggtc tcgctgaagg
     1741 gaaaggtcat cgatatcgag gaggttgtca agaaggggat gttgaaaaag cgcggtagcg
     1801 cgtacatcct tcctctttcg accgatgccc gcggcaaaat agtggtgggg agcgccatcg
     1861 tcctcttcca attcatcacg ccgccgccca agccccccaa attgcgattg cctcgctcca
     1921 tcagaggatc atggctccag atgatagact ggccactggc cgctatccag ggaagcatat
     1981 tcatcgtggg ctatgcgttc attttctgga tgtatggtct tcccgatcca gcccccaaga
     2041 cccttgaaca gatagacaat cgctttgccc gcttgatagc tgatgtcgat cagatgaaag
     2101 agaaggaaaa accggttgat gaaacgccca ccactgacga aggtgcggcc aagaccgcaa
     2161 agaaagaaga gaaaaaaagc gacgccggcg ccgacgaacc tgtcaaaaag agaaagggac
     2221 cgcgtgatgc ggcgacccgt gcgcaggagg aggcgatgcg gctctctgaa atgaagaaga
     2281 aagtcgctgg gttaggtctg atgaagatgc ttggctccac cggaggagac agttcgttcg
     2341 gcatagtatc tgatgtgctg ggagaagggg caaaggaccg agatatagat tcggcgcttt
     2401 caggcgttaa acagataggt gtggcgaccg atgccggtca gcgaagccgg aaaggggacg
     2461 ccggcgcgac cgaaacggcg aagatcgacg acctgaaggt ggccgacagc gcaggtaagg
     2521 taaaggtggg tgccgtgaag gagaaggaga tcaccggaag caccaaggta ggcgctcccg
     2581 aaatagaagg gacccttgac cgtggtgcca tcaacaagat agtgaagcag aacagccccg
     2641 ccatacagcg ctgttatctg aatgccttga agacgaatcc gcagctgaag ggaaggatcg
     2701 aggtgacctg gatgatcaac gcggacggtc gcgcggaatc tgttcagata accgaagata
     2761 cggtgaagga cggaggcgtg acctcctgca tcaagagtgt ggtctcgcgc tggagattcc
     2821 ccaagccgga agatccgggt atcgttgcct tcccgttcat attcacgccg gcggagtaac
     2881 ggatgcggag aaaaggaggt gccatcatga aatggcttgc tctgacctgt gcggtggcca
     2941 tgctcatgat acctctctct acacaggcgg ctcaggaaac ggcatccgtc agtgtcgatc
     3001 agatgcgaag cgatatcgat ggctgggcgc ggcgcgtgac ggagatagag gcgcagatac
     3061 agaagatgaa tgcacaggcg gtcaacgaga aggatatcaa gtggaaactg tgcctagatg
     3121 acatcctcgc aacgataaaa ggggtttcgg ccagcataat ctcggctcgg aaccgtatgg
     3181 aggacatgat ccgttccgag aagaaagatg cggcacagat ccagttcacg cttgtaaaag
     3241 ggcttgcgga cgcggcggag cgggctttta ccgaatcgca agggtgtccg cggcagttga
     3301 cccgtgtcga tacctcatcc accattgaga aggaggagga caagtcgatc accggtacgc
     3361 gtggtgacaa aggcggcata ggggacgcca tggggcagga tttcacggag gatttcgcca
     3421 cggagcgcga ccccaacgat ctgggcgcgg aagacccgat agacggcgcc ggcaccgaca
     3481 cccccg
//
LOCUS       DAOQUE010000295         3484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_30808_length_3484_cov_3.715661, whole genome shotgun sequence.
ACCESSION   DAOQUE010000295 DAOQUE010000000
VERSION     DAOQUE010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30808_length_3484_cov_3.715661"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKH10_09070"
     CDS             complement(<1..436)
                     /locus_tag="PKH10_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28313.1"
                     /translation="MIKIRPILILVLLFSVSYGFQNPEPGEQTIEAHGGVGAYGLTSC
                     ETLHIYDYRVATAQYKKRFDDRFQIGAAAAYINRVERYAVNINTGAGVPLDPREVLNL
                     GGVMMSASFLHEYVEVTLGAGFFAGKDQNGDRILPWPHAIIRA"
     gene            complement(433..1752)
                     /locus_tag="PKH10_09075"
     CDS             complement(433..1752)
                     /locus_tag="PKH10_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28314.1"
                     /translation="MKWLVIIFAFLFITACDGTKKIATDQESPMDTDTPTNTDTSQDG
                     DIGIEEEPASDDMSPPDDDMSAMENESTMDDDTLDNESVTDSDGLICRIDDERCGEYD
                     QAVEICTAQGWQIKQNCDALEDCYQYIEDDVYVAACHDMSEPDDKDECHVNDTMCSGS
                     EMFEDSRVIICTEGTDWYTLEYCEGDIYDGCREEFASGAYYAECVFKGCSGHEGELAC
                     GNNSLYICEEGYWVFKEDCGPSKECHGSIGMAECGEIWQDDVKPNIYLYPTAPLDITV
                     QIGFPQGGRVTVSEPLYSDGWDVHVTPDGLIDDQWRFLFYESVQPNGWQKERGYLVAT
                     DEMERFFRNDLATRGFVGQEIEDFIEWWMPLLAGTGYLIVYPQDDAVIRTVETLELSV
                     APDNLLRHRYVIETTSEPITIEPPADPAPFIRDGFTVTEWGVIRLQP"
     gene            1966..2544
                     /locus_tag="PKH10_09080"
     CDS             1966..2544
                     /locus_tag="PKH10_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HNT28315.1"
                     /translation="MTDGFIPAHGGYRKLLSYQRAEIVYDATVRFCERFLKKGDRTID
                     QMVQAARSGKQNIIEGSMASGTSKETEIKLTNVARASLEELLADYRDFLRLRKIEEWG
                     RDHPYARRLSELIRTPNANYETFRKGIEHDDPAIAANAILGLIKITNYLLDKQIARLE
                     RAFLEEGGLRERMTKARLSAREKQGKGTKGTK"
     gene            complement(2563..>3484)
                     /gene="trmFO"
                     /locus_tag="PKH10_09085"
     CDS             complement(2563..>3484)
                     /gene="trmFO"
                     /locus_tag="PKH10_09085"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNT28316.1"
                     /translation="ADLYIIATGPLTSPQLLASISTDNHYFYDAIAPIVSAEGLDPTK
                     LFWADRYEKGQPDFLNCALTRQEYDRFVDALLAADKLPYADHERPQFFDRCMPIETMA
                     ERGRNTLSFGPFRPVGFEVEGQRPYAVVQLRAENADKSAFNLVGCQTRMRHHAQREVF
                     RLLPGLENAEFLRFGSVHRNSYINAPELLADGLNLLKHPDTYVAGQLSGVEGYNESIF
                     SGLYTALTIIARFEEKKLPPPPAATMSGGLLRKLRAPAGRFAPVNANFSIIDNPTRLR
                     KRDRKLFHVADGVRQFREWWAGVEKGTQAT"
BASE COUNT          730 a          952 c         1057 g          745 t
ORIGIN      
        1 ccgcccggat aatggcgtgc ggccacggga gtatccggtc gccattctgg tccttcccgg
       61 cgaaaaagcc ggcgccgagc gtcacctcga catattcgtg caggaacgat gccgacatca
      121 tgacgccgcc gagattgagc acctcccgcg ggtccagcgg cacgcctgct ccggtgttga
      181 tgttgaccgc gtagcgttcg acgcggttga tgtaggccgc cgccgcgccg atctgaaaac
      241 ggtcatcgaa tcgttttttg tattgggcgg tcgcgacgcg gtagtcgtag atgtggagcg
      301 tttcgcagga ggtcaggcca taggcgccga cgccgccgtg cgcctcgatc gtctgttcgc
      361 cgggttcggg attctgaaaa ccgtaactca ccgagaaaag aagaacgaga ataaggatcg
      421 gtcgtatctt tatcatggtt gcaaccgtat cacgccccat tcggtgacgg tgaagccgtc
      481 gcggatgaag ggcgcggggt cggcaggagg ttcgatggtg atcggttcgg aggtcgtttc
      541 gatgacataa cggtgccgca gaaggttgtc gggggcgacc gagagttcca gtgtttccac
      601 ggtgcggatg acggcgtcgt cctgcggata gacgatgaga tagcccgtcc cggcgaggag
      661 cggcatccac cattcaatga aatcctctat ctcctgcccg acgaagccgc gcgtcgcaag
      721 gtcgttgcgg aagaaccgct ccatttcgtc ggtcgcgacg aggtagcccc gttccttctg
      781 ccagccgttc ggctgaacgc tctcgtagaa caggaaccgc cactggtcgt cgatgaggcc
      841 gtcgggcgtc acatggacat cccagccgtc gctgtagagc ggttccgaca ccgtgacgcg
      901 gcccccctgc ggaaaaccaa tctgcacggt gatatccagc ggtgccgtgg ggtagaggta
      961 gatattgggc ttcacatcat cctgccatat ctcgccgcat tcggccatgc cgatagaacc
     1021 atgacattcc ttcgagggac cacaatcttc tttgaatacc cagtagccct cctcgcagat
     1081 gtaaaggctg ttgttcccgc aggcaagttc gccctcatgg ccggaacaac ccttgaaaac
     1141 acactctgca taatatgcac cactcgcgaa ttcttcgcgg caaccatcgt atatgtcgcc
     1201 ttcgcaatat tcgagcgtgt accagtctgt gccttcggta caaatgatca cgcgactatc
     1261 ctcgaacatt tcgcttcccg aacacatcgt gtcgttgaca tgacattcgt ctttgtcgtc
     1321 gggttcactc atatcatggc atgcagccac atagacatca tcttcgatat attggtagca
     1381 atcctccagc gcatcgcaat tttgtttgat ctgccaaccc tgcgcggtgc atatttcaac
     1441 ggcttggtca tattcgccgc agcgctcgtc atcaatgcga cagatcaaac cgtcactatc
     1501 ggtgaccgat tcgttgtcga gggtatcgtc gtccatcgtg ctctcatttt ccatcgccga
     1561 catgtcatcg tcgggtggtg acatatcatc ggatgctggt tcttcttcga tcccaatatc
     1621 accgtcttgt gaggtatcgg tgtttgtcgg agtatcggta tccatcggcg actcttggtc
     1681 ggttgctatc ttttttgtcc cgtcgcatgc cgtgatgaag agaaaggcga agatgatgac
     1741 aagccatttc atgaaacacc cctctgttga aagaatagac ccgggagtaa aataacattt
     1801 ccgaacggga agtcaatcgg aaaaaattga aaaagaggtg gggaaagaat aaagggaccc
     1861 aagagacggc agtgacccaa gggacggcgg tgacccaaga gacaaaggga tcgtcgctta
     1921 tgtccctcag gtcgcttgtg tcgctttctt ctgtgaggtt ttatcatgac cgacggtttc
     1981 attccggccc acggtggcta ccggaaatta ctttcctatc agcgggccga gatcgtctat
     2041 gatgctacgg tccgcttctg cgaacgtttc ctgaaaaaag gggaccgcac cattgatcag
     2101 atggtacagg cggcgcggtc cggcaaacaa aatatcatcg agggaagcat ggcgtcgggg
     2161 acctcaaagg aaaccgagat aaaactgacc aatgtcgcgc gggcgagtct cgaagagctt
     2221 ttggccgact atcgcgactt tctgcgacta aggaagatag aagagtgggg gcgtgatcat
     2281 ccgtatgccc gccggttgag cgaactgata cgcacaccga acgcgaatta tgagactttc
     2341 agaaaaggca tcgagcacga tgatcccgcc atcgcggcaa atgcgatcct cgggctgatc
     2401 aaaataacca attatctact cgataaacag atcgcgcgac tagagcgggc gttcctcgaa
     2461 gagggtggct tgcgcgaacg gatgaccaag gcacggttga gcgctcggga gaagcagggg
     2521 aaggggacca aagggacaaa ataattctgt cgcttgcgtc gcttaagtcg cttgtgtccc
     2581 tttctccacg ccagcccacc attccctgaa ctgtctgacc ccgtcggcca catggaacag
     2641 tttgcggtcg cgtttccgga gccgcgtcgg gttgtcgatg atggagaaat tggcgttgac
     2701 cggcgcgaaa cggcccgccg gggcgcgcag tttgcggagc agtccgccgc tcatcgtcgc
     2761 ggcgggcggc ggcgggagtt tcttttcctc gaagcgggcg atgatggtca gcgcggtata
     2821 gagccccgag aagatcgatt cgttgtagcc ctcgacgcct gagagttgtc ccgcgacata
     2881 ggtgtcgggg tgtttcagca ggttcagccc gtcggcgagc agttcggggg cgttgatgta
     2941 ggaattgcgg tggaccgaac cgaaccgcag gaactcggcg ttctcgagac ccggcaggag
     3001 ccggaacacc tcgcgctggg cgtgatggcg catccgggtc tggcaaccga cgaggttaaa
     3061 ggcgcttttg tcggcgttct cggcgcgcag ttgcacgacg gcgtacgggc gttgcccctc
     3121 gacctcgaac ccgaccgggc ggaagggacc gaacgaaagg gtgttcctgc cgcgttcggc
     3181 catcgtctct atcggcatgc accggtcgaa gaactgcggc cgctcgtgat cggcgtaggg
     3241 gagtttgtcg gcggcgagaa gcgcatcgac gaaccggtcg tactcctgcc gggtgagcgc
     3301 gcagttcagg aaatcgggct ggcccttctc gtaccggtcg gcccagaaga gtttggtcgg
     3361 gtcgagcccc tcggcgctca cgatgggggc tatggcgtca tagaaatagt ggttgtcggt
     3421 cgagatgctc gcgagcagtt gcggcgaggt cagagggccg gtcgctatga tgtaaaggtc
     3481 ggcg
//
LOCUS       DAOQUE010000296         3481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_30850_length_3481_cov_4.201109, whole genome shotgun sequence.
ACCESSION   DAOQUE010000296 DAOQUE010000000
VERSION     DAOQUE010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30850_length_3481_cov_4.201109"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..337
                     /locus_tag="PKH10_09090"
     CDS             29..337
                     /locus_tag="PKH10_09090"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNT28317.1"
                     /translation="MFRTVTLVALFTTLLITVLLFALKNGRPVPVDLVVTRLDTVPLW
                     VVMVAACAVGSTLATLVFAVVFFSLYFAKRSVEKELQKTRAELAALTVKDHADKGVAR
                     "
     gene            337..1428
                     /locus_tag="PKH10_09095"
     CDS             337..1428
                     /locus_tag="PKH10_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28318.1"
                     /translation="MDLFHPHTREILVASALLVALLLILLRMIWSRRRTALRIKTDML
                     SVLPSLERLAEGDRAELSKWLRRAARRYPDEVRTFILLGDLLRAEGPLKAVFLHRTLL
                     FRKKLSTADRASILLSLGRDHRALEDDQKALASLKQSLAAEKREETLKELVSLETAMG
                     LFDDALYHCKELNRLAGLDRNEGLRAVMLDALTVAVSRNRPEEARRWGEMVARAENDT
                     ALALLIPCVLAAWEGKVEQARPLAEKLIERYPEEECTLRYLLLQTTTGMAFSRLLPGP
                     LGSVFVALCDETRVLTATEMAPIEKKSALYYHLAARTVPPGETRDLLAAAGRRERLFA
                     CAACGGRFEGMRFSCPGCGLPVTVRPGVV"
     gene            1457..2545
                     /locus_tag="PKH10_09100"
     CDS             1457..2545
                     /locus_tag="PKH10_09100"
                     /inference="COORDINATES: protein motif:HMM:NF016584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0007275 - multicellular organism
                     development [Evidence IEA];
                     GO_process: GO:0030178 - negative regulation of Wnt
                     signaling pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dickkopf N-terminal cysteine-rich
                     domain-containing protein"
                     /protein_id="HNT28319.1"
                     /translation="MSERPRMILTGMLVLALMLGACEGTKSEKAKMPVDEFFQTYLQT
                     ICGSAAEYSGGIITSANKDSCVDSIKESLLVWDFFHTGRKTVFKQKIYLLRLAEKREW
                     LSVDADQAQKCFDIISQMHPYNPLEIRLFDIPDCALAFKGLKIINDQCFQDEECDNGW
                     CNLAAGACPGWCVLYRRQGEPCNENLDRCEPGYTCLSSGCSRLSTGAKGEPCMSDDHC
                     ASYLYCKKTEGDDIGICFEKKGIGKTCAEDNECIVGLTCIDEQCQGPEIPNSLGADCS
                     ASKVCNYFSRLECGVNGETYACRAFPAEEYAACTLQCDKKFYCDPFDHKCSYFKGEGE
                     PCGQYYQCSTLYCAKGGVCEMPECEQIF"
     gene            2776..3093
                     /locus_tag="PKH10_09105"
     CDS             2776..3093
                     /locus_tag="PKH10_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28320.1"
                     /translation="MDLSASWCGPCAELTERLNTLQERFGGRVRFLMVVQKGDPPELQ
                     PGRPAYPIYTLEEAPTEMGIRPPEVLPTVLFFAGDGSLFGEIAGLYPALFYYGVLADL
                     TAR"
     gene            complement(3079..>3481)
                     /locus_tag="PKH10_09110"
     CDS             complement(3079..>3481)
                     /locus_tag="PKH10_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28321.1"
                     /translation="RWWCSVRPAICIDRSKLYFLSDVAGIIPQSGDLPLEYAEGYLNS
                     DLILFYYTLKFPHHNKFLKKNFNSIPFTPAKKQLQEVVSAAVRAIRSGDDEKGARDLN
                     RSIYQLFRLTSDEIRSIERYVERYRARSSGR"
BASE COUNT          661 a         1037 c         1094 g          689 t
ORIGIN      
        1 actatagccc cggtcgggag gaggccccat gttcaggacc gtaacgctcg tggcgctgtt
       61 cacgacactg ctcatcaccg tcctgctgtt cgccctcaag aacggccggc cggtgccggt
      121 cgacctcgtg gtcacccggc tcgatacggt gccgctgtgg gtggtgatgg tcgccgcgtg
      181 cgccgttgga tcgacgctcg ccacgcttgt gttcgccgtg gtgttcttca gtctctactt
      241 cgccaaaagg tcggtcgaga aggaattgca gaagacccgc gccgaactcg cggcactcac
      301 cgtgaaggat cacgccgata agggtgttgc ccgctgatgg acctctttca tccgcacacc
      361 cgggagatac tcgtcgcgtc ggcactgctc gtcgcgctcc tgctcatcct gctcaggatg
      421 atctggtcgc gtcggcggac cgccctgcgg ataaagaccg acatgctctc ggtcctcccg
      481 tcgcttgagc gactcgccga aggggaccgc gccgaactct cgaaatggct gcgtcgcgcc
      541 gcacggcggt accccgacga ggtgcgcacc ttcatcctgc tcggcgacct gctccgcgcc
      601 gaaggtccgc tcaaggcggt gttcctgcac cgtaccctgc tgttccgcaa gaaactctcg
      661 acggccgacc gggcttcgat actgctcagt ctcgggcgcg accatcgggc gctcgaggac
      721 gaccagaagg cgctcgcctc cctcaaacag agccttgcgg cggaaaaaag agaggagacc
      781 ctgaaagaac tggtgtcgct tgaaacggcg atggggcttt tcgacgacgc gctctaccat
      841 tgcaaggaac tcaaccggtt ggcgggactt gaccgcaacg aaggcctgcg cgcggtgatg
      901 ctcgacgccc tcaccgtcgc ggtctcccgg aaccgccccg aagaggcgcg gcgctggggt
      961 gaaatggtgg cgcgcgcgga gaacgacacc gcgctcgcgc ttttgatacc ctgtgtcctc
     1021 gcggcatggg agggcaaggt cgaacaggcg cggcctctcg cggaaaaact catcgaacgg
     1081 taccccgaag aggaatgcac gctacgctat ctgctcctgc agacgaccac cggcatggcg
     1141 ttctcgcgcc ttctgcccgg gccgttgggt tcggtctttg tggcactctg cgatgaaaca
     1201 cgggtactta cggcgaccga gatggcccct attgaaaaaa agagcgcgct gtactatcat
     1261 ctggcggcgc ggaccgtgcc accgggagag accagagatc tcctcgccgc cgccgggagg
     1321 cgcgagcggc tgttcgcctg tgcggcatgc ggaggccgct tcgaggggat gcggttctcc
     1381 tgtccgggat gcggactccc agtgacggtg cgaccgggtg tggtctgacg aatgttgaga
     1441 gtgacggagg ataccgatga gtgaacgtcc gagaatgata ctgaccggca tgcttgtgct
     1501 cgccctcatg ctgggagcgt gcgagggcac gaagagcgaa aaggcgaaga tgccggtcga
     1561 cgaattcttc cagacctacc tgcagacgat ctgcgggagc gccgccgagt acagcggcgg
     1621 tatcatcacc tccgcgaaca aagactcctg cgtggacagc atcaaggaga gtctgctcgt
     1681 ctgggatttc ttccatacgg ggcgcaagac ggtcttcaag cagaagatat atctgctgcg
     1741 tctcgccgag aaacgggaat ggctgagcgt ggacgccgat caggcgcaga aatgcttcga
     1801 catcatctcc cagatgcatc cctacaaccc gctcgagata cgactgttcg acattcccga
     1861 ttgcgcgctc gccttcaaag ggctgaagat catcaacgac cagtgcttcc aggacgagga
     1921 atgcgacaac ggttggtgca acctcgccgc cggcgcctgt ccgggatggt gcgtactgta
     1981 tcgccggcag ggggagccat gcaatgagaa cctcgatcgc tgtgagccgg gatatacctg
     2041 tctgagttcg ggctgttccc ggctttcgac cggcgccaaa ggagaaccct gtatgagcga
     2101 cgatcactgc gcgagttatc tctattgcaa gaagacggaa ggcgatgaca tagggatctg
     2161 tttcgaaaag aagggtatcg gcaagacctg cgccgaggat aacgagtgca tcgtcggtct
     2221 cacctgtatc gatgagcagt gtcaggggcc ggagataccc aacagtctcg gcgccgactg
     2281 cagcgcctcg aaggtctgca attatttctc ccgtctcgag tgcggcgtga acggggagac
     2341 ctatgcctgt cgcgccttcc cggcggaaga gtatgcggcc tgcactctcc agtgcgacaa
     2401 gaagttctac tgcgatccgt tcgatcataa gtgctcctat ttcaaaggcg aaggggagcc
     2461 gtgcggacag tactatcagt gttcaacgct ttattgcgcc aaaggcggcg tatgcgaaat
     2521 gcctgaatgc gaacagatat tctagcggca ttcctgctcg cgaccctttt tttcggggcc
     2581 tgtgcctcct cgggtgccgg tgccgcttcc ggtgcgccgg tgccttcatc ctcgccggtc
     2641 gtcgcaagga aagaaggggc gcctattgtg cctgaaaagc gacgggagac gcttgccgac
     2701 cgagtgcttc tcaaagaccc ggagagcggt cgcgtgtcgt cgttggccga actaacgggc
     2761 ggttcgccct ctctgatgga tctttcggca agttggtgcg ggccgtgcgc cgaactgacc
     2821 gagcgtctca acacgctaca ggaacggttc ggcggtcggg tccgtttcct gatggtcgtt
     2881 cagaaggggg acccccccga actgcaaccc ggccgcccgg catatccgat ctacaccttg
     2941 gaggaagcgc cgacggaaat gggcattcgg cctccggagg tgttgccgac cgtcctgttt
     3001 tttgccgggg acggctccct cttcggcgag atcgccgggc tgtacccggc gttgttctat
     3061 tacggggtgc ttgccgatct aacggcccga tgaccgcgcg cgatagcgtt cgacataccg
     3121 ctcgatgctg cggatctcgt cgctggtcaa tctgaacagt tgatagatgc tccggttgag
     3181 gtcccgggcc cctttctcgt cgtcacccga ccggatggcg cgcaccgccg ccgagaccac
     3241 ctcctgcaac tgtttttttg cgggggtgaa agggatgctg ttgaagttct tcttgaggaa
     3301 cttattgtgg tgcgggaatt tgagcgtgta atagaacagt atcaggtccg agttgagata
     3361 gccctcggca tattcgagcg gcaggtcgcc actctgcggg atgataccgg cgacatcgct
     3421 gaggaagtag agttttgatc gatctatgca gatcgccgga cgcaccgaac accaccatcg
     3481 t
//
LOCUS       DAOQUE010000297         3477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_30898_length_3477_cov_3.747516, whole genome shotgun sequence.
ACCESSION   DAOQUE010000297 DAOQUE010000000
VERSION     DAOQUE010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30898_length_3477_cov_3.747516"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PKH10_09115"
     CDS             complement(<1..973)
                     /locus_tag="PKH10_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT28322.1"
                     /translation="MAKEQVKKENDAVKNGEQYGADSIQVLKGLEAVRKRPGMYIGDT
                     DDGSGLHHMIFEVVDNSVDETMAGFCDKISVYLHVDGSVTIKDNGRGIPVDMHPEEKR
                     SAAEVAMTVLHAGGKFDQNSYKVSGGLHGVGVSVVNALSEWLRLEIRRQGFVWVQEYR
                     RGDPVAPLKKTEPTDETGTKISFKPDPKVFTNTEFSLARVTNRLREMAFLNSGARIKL
                     LDERTGEEKLFHYEGGIVEFIAFLNQNRTPLHKDVIRIARTEGPHAVEIALQWTDAYV
                     ENVLCYTNNINNRDGGTHLSGFKLGITKVFQKYIAENRKNAKVQINGD"
     gene            complement(1158..2579)
                     /locus_tag="PKH10_09120"
     CDS             complement(1158..2579)
                     /locus_tag="PKH10_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28323.1"
                     /translation="MRALRLSLLTTLLFPLMLYSSQKHGAPHCTASLVLPAGLHLLET
                     QECFSTDLADAASSDPDALRRQLDRFPDTPATAGYRYWSYLRSERFLPALLTLIDLVN
                     RSGDPDTAAQFSDEIAFLVGAGVRHVDPQADLFGRAENGRTVSVLSDRFFFILDQVVS
                     LYAEGKRGRAQRDAWLLYRALTLFFSTADLSIDQSEDPFFSSLLQRYRQLWKSDLLLS
                     YLGVFSFLTVGDTTFLPSFPLYRDRLAGIVDLPERRTGGGAGSAVEEIRRFTGRLFID
                     SLWVLPTVTVVDTDDIHRWYAAMRGTSRPDTDAGCAVFDERHRMLAPYRHDLFDALTA
                     NRALTLLEGCDPAYKVPFVIAGERLFLSSRDRWLSSSAVGYHLLGSGEPSYRESADLM
                     SALLLASLDQYLEERRNGGALSPFGEMLRYAYGTGRLTEFRDTFFLYPRYRSQSTVFT
                     PQKTRELTDFISDFFIDDRSDTD"
     gene            complement(2595..3101)
                     /locus_tag="PKH10_09125"
     CDS             complement(2595..3101)
                     /locus_tag="PKH10_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28324.1"
                     /translation="MPISGELFTQAGRHARPLFCAVLTAILSVSCVWSPDKKDGSALW
                     DQRVDELAATDKDAVTIDTLNGQDAMIGTDEDLLELPDADSPACMGKTCAAHADCKTT
                     GCAATFCSAVLGELYPDNPNVCAVRCDPANGDDDCPEDLVCNAGIQMAPSNIDIDGAK
                     GLCAPPTN"
     gene            complement(3103..3357)
                     /locus_tag="PKH10_09130"
     CDS             complement(3103..3357)
                     /locus_tag="PKH10_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNT28325.1"
                     /translation="MATQLYVGNVSYQMTEESLKKMFSEFGEVTSAKIVVDRETGRSK
                     GFGFVEMADAQAADRAIESLNDQEVDGRRIKVNIARPKKF"
BASE COUNT          732 a         1004 c         1051 g          690 t
ORIGIN      
        1 cgtcgccgtt tatctgcacc ttcgcgttct tgcggttctc ggcgatatat ttctggaaca
       61 ccttggtgat gcccagtttg aagcccgaca gatgagtgcc accgtcgcgg ttgttgatgt
      121 tgttggtgta gcacaggaca ttctcgacat aggcgtcggt ccactgcagg gctatctcga
      181 ccgcgtgcgg cccctcggtc cgcgcgatac ggatgacatc tttgtggagc ggggtccggt
      241 tctgattgag gaaggcgatg aactcgacga tgccgccctc gtagtggaac agtttctcct
      301 ctccggtccg ctcgtcgagt agttttatcc gcgcgccgct gttgagaaaa gccatctcgc
      361 ggagtcggtt ggtgacccgc gccagagaga attcggtgtt ggtgaagacc ttgggatcgg
      421 gcttgaacga tatcttcgtc ccggtctcat cggtcggttc ggtctttttg agcggggcga
      481 ccggatcgcc gcgccggtat tcctgcaccc acacgaaccc ctgccgccgt atctcgagcc
      541 gtagccactc cgacaaggcg ttgacgaccg atacgccgac gccatgcaga ccgcccgaca
      601 ccttgtagga gttctggtcg aacttgccgc cggcgtgcaa caccgtcatc gcgacctcgg
      661 cggcgctccg cttctcctcg gggtgcatat cgaccgggat gccgcgaccg ttgtccttta
      721 tcgtgaccga gccgtcgaca tgcaggtaga ccgatatctt atcgcagaag ccggccatcg
      781 tctcgtcgac cgagttgtcc acgacctcga agatcatgtg gtgcagaccg ctgccgtcat
      841 cggtatcgcc gatgtacatg ccgggccgct tgcggaccgc ttcgagtccc ttcaggacct
      901 gtatgctgtc cgcgccgtac tgctcgccgt tctttaccgc atcgttctct ttcttcacct
      961 gttctttcgc catgggagac ctcgtaaaaa accgttgtcg accgattata gaacgcgagg
     1021 ggcgaaaagc aactttttct gccgcgaacc tgctctaaaa gcgtagtgat ataaggcgga
     1081 tagcgaatcg aataaaaata ttttaaaaaa ccgcgtagtg aaagactatg ttactgtatt
     1141 tacagggata tctcttctca gtcggtgtca gaccgatcgt cgataaagaa atctgaaata
     1201 aaatcagtta gttctcttgt tttttgcgga gtgaacaccg tcgactggga acgataccgg
     1261 gggtagagaa aaaaagtgtc gcggaactcg gtcagacgcc ccgttccgta cgcatagcgg
     1321 agcatttcgc cgaaggggga caacgcaccg ccgttccgcc gttcttcgag gtactggtcc
     1381 agcgatgcca gaagaagtgc gctcatcaga tcggccgatt cccggtagga aggttctccg
     1441 cttccgagca gatgatagcc gaccgccgaa gaggaaagcc atcgatcgcg ggaggagagg
     1501 aacagccgtt cgcctgctat cacgaagggc accttatacg ccgggtcaca cccctcgagc
     1561 agtgtgagtg cgcgattcgc ggtaagggcg tcgaataggt cgtgccggta cggcgccagc
     1621 atgcggtgcc gttcatcgaa caccgcgcaa ccggcatcgg tgtccggcct tgatgttccc
     1681 cgcatcgctg cataccaccg gtggatatca tcggtatcga cgaccgtcac ggtcggaagg
     1741 acccaaagac tgtcgatgaa caggcgaccc gtgaagcgcc ggatctcttc gacggcggac
     1801 ccggcccctc cccctgtgcg tcgttccggc agatcgacga tgcctgcaag acgatcgcga
     1861 tacaacggaa aggaaggcag gaaggtcgtg tcgccgacgg tgagaaaact gaaaacgccg
     1921 agataggaca acagcagatc gctcttccaa agttgtcgat agcgttgcag aagcgaagag
     1981 aagaacgggt cctcgctttg atctatggaa aggtccgcgg tcgaaaagaa cagcgtcagc
     2041 gcacggtaca acagccacgc gtcccgttgt gcgcggcctc gtttcccttc ggcatagagc
     2101 gacaccacct gatcgagtat gaaaaagaac cgatcggaaa ggacactgac ggtccgaccg
     2161 ttctcagcgc gaccgaaaag atcggcctgc ggatcgacgt ggcggacgcc ggcgccaacg
     2221 agaaaggcga tctcatcgct gaattgcgcc gccgtatcgg gatcgccgga ccggttcacg
     2281 aggtcgatga gggtaaggag cgcggggagg aaccgttcgg agcgcaggta ggaccagtag
     2341 cggtacccgg ccgtcgccgg cgtgtcgggg aaccggtcga gttgtcgtct caacgcgtcg
     2401 gggtcggatg aggcggcgtc ggcgaggtcg gtcgagaaac actcctgcgt ctccagcagg
     2461 tgcaggcccg ccggaagaac gagcgaggcg gtgcagtgag gcgctccgtg cttttgggag
     2521 gaataaagca tcaaagggaa caggagcgtg gtcaggagag aaagtcggag cgcgcgcata
     2581 ccgggaacgg ggggtcagtt cgtcggaggc gcgcagagcc ctttcgcgcc atcgatgtcg
     2641 atgttgctgg gagccatctg tatcccggcg ttgcagacga ggtcttcggg gcagtcgtcg
     2701 tcaccgttgg ccgggtcgca ccgtaccgcg cagacattcg gattatccgg gtatagttcg
     2761 cccagcaccg ccgaacagaa ggtcgctgcg catcccgtcg tcttgcagtc ggcgtgagcc
     2821 gcgcaggtct tgcccatgca ggcaggggaa tcggcatcag gcaattcgag gagatcttcg
     2881 tcggtgccga tcatcgcatc ctgtccgttc aaggtgtcga tcgtcacggc gtccttatcg
     2941 gtcgccgcca gttcgtcgac acgctggtcc cagagagcgg agccgtcctt tttatccggg
     3001 gaccagacac aggaaacaga aaggatcgcg gtcagcaccg cgcaaaaaag aggccgggca
     3061 tgacgcccgg cctgcgtgaa cagctctcct gatatcggca tatcagaatt tcttgggacg
     3121 cgcgatgttg accttgatgc gacgaccgtc tacttcctga tcgttcaggc tctcgatggc
     3181 gcgatccgcc gcctgagcgt cggccatctc gacgaaaccg aaccccttcg aacgaccggt
     3241 ctcccgatcc accacgatct tcgccgaggt gacctcgccg aactccgaga acatcttctt
     3301 cagcgattcc tcggtcatct ggtaactcac gttcccaacg tacaactgtg tggccataac
     3361 gaaaccctca aaacaaaaaa accataatac gccccctgct cagacccggc atcactgtga
     3421 cgaaaggcca gcaggatggg ggcagggaag ttgaatagga tgcgcagcac gctgatg
//
LOCUS       DAOQUE010000298         3475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_30925_length_3475_cov_3.709064, whole genome shotgun sequence.
ACCESSION   DAOQUE010000298 DAOQUE010000000
VERSION     DAOQUE010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30925_length_3475_cov_3.709064"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKH10_09135"
     CDS             <1..192
                     /locus_tag="PKH10_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28326.1"
                     /translation="GDIAAIRDSDLFPTLAAKLGVTPGEAARLLWDTYHPWQIWAILA
                     SVGFISMVGLFWLDRNARR"
     gene            209..1639
                     /gene="cysS"
                     /locus_tag="PKH10_09140"
     CDS             209..1639
                     /gene="cysS"
                     /locus_tag="PKH10_09140"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNT28327.1"
                     /translation="MLRVYNTLSEKKEDFIPLQEGKVGLYACGMTVYDKPHIGHARKE
                     VAIDMVVSYLRYSGYAVTYVRNFTDVDDNIIRRANDRGISCDELTAENISAFYRAVDA
                     LGLTRPDVEPKATEHMAEIIGIVEKLIEKGHAYAAPDGSVYFAVESFKGYGKLSNRKL
                     DELLAGTRFEPEAGKRNPLDFALWKSAKPGEPSWDSPWGGGRPGWHIECSAMSSKYLG
                     DSFDIHAGGRDLIFPHHENEIAQSEAASGRPFVKYWVHNGFLTVEGEKMSKSLGNFIT
                     VDDALDQYHPEVLRLFMLSTHYRTPIDFSYAGLAEAERRVCYFYETIKNIKKLAGDAA
                     AKTTERADAFRAEFRESMDDDFNSPKVMAALFGICKFLNESAVSKKTRPAPDEAAGYL
                     TAIAEAGKVFGLLQADPATIIEAIKDLQLERAKLTRREIDALIAERNAHRKEKDFAAA
                     DAVRAKLDALGILIKDTPDGTEWSFK"
     gene            1758..2843
                     /locus_tag="PKH10_09145"
     CDS             1758..2843
                     /locus_tag="PKH10_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28328.1"
                     /translation="MNYRRSLILAALASLAALLSACAEPQDDRVSCVTSFDCPLGWFC
                     EDSWCVQNPDQSAGGDTKNDIDTSVDIDTATAEDDTLPDTAEGSDLNDDGVIPDEQGD
                     DAVIVDDTDEPTVEGDALIPDDDTGATDDGTDTDTATDGDIVTDTATDADAVPPNTGA
                     TCSVAGDCVGGATCGQIFTGQTTSYCYLSCAWPNESICAGTAWPECAVISSWALCLAT
                     ATVGGNFTALVDSFQQGNNISLTINGTNHIQSGCVASLSGSTWILQCSKVLESTPNTI
                     QLDTVFYWDTGEHNTDGTVANAEGRAYQTTFVGTTNEVSERWVRAVFTVNDGTLTLTQ
                     AGTAAGSTVAGTINFTGNAYDAQLTVQ"
     gene            2905..3300
                     /locus_tag="PKH10_09150"
     CDS             2905..3300
                     /locus_tag="PKH10_09150"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNT28329.1"
                     /translation="MFRKMLKSKLHRLRITNADLIYQGSITIDAELLERADIRSYEFV
                     DVWNVSNGARFETYAIPGERGKREVCVNGAAARLVNRGDIIIVASHRYLTEEEAGDLT
                     PTIVFVDENNHPLSEAPWAKNTAASNGPR"
     gene            3261..>3475
                     /locus_tag="PKH10_09155"
     CDS             3261..>3475
                     /locus_tag="PKH10_09155"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT28330.1"
                     /translation="MGKEHRRFKRAEIAIRIRLRESDTDIEASFALRDLSEGGLFVHS
                     PILWEPGEKFELSFMLPGSDREIHATG"
BASE COUNT          726 a         1122 c         1034 g          593 t
ORIGIN      
        1 ggcgatatcg cggccatcag ggacagcgac cttttcccga cgctcgccgc gaaactgggc
       61 gtcacccccg gcgaggcggc gcgactgctc tgggacacct accacccgtg gcagatatgg
      121 gcgatactcg cctcggtcgg tttcatttcg atggtggggc tcttctggct tgaccggaac
      181 gcccgcagat aacgatacgg aggtcgccat gcttcgcgtc tacaacacgc tctcggaaaa
      241 gaaagaggac ttcatccccc tgcaggaagg caaggtcggt ctctacgcct gcggcatgac
      301 ggtctacgat aagccgcata tcggccacgc ccgcaaggag gtcgccatcg acatggtggt
      361 gagttacctg cgctacagcg ggtatgcggt gacctatgtc cgcaacttca ccgatgtcga
      421 cgacaatatt atccggcggg cgaatgatcg tggtatctcg tgcgatgagt tgaccgctga
      481 gaatatatcc gccttctacc gcgccgtcga tgcgctcggt ctcacccggc ccgatgtgga
      541 gcccaaggcg accgagcaca tggccgagat catcggtatc gtcgagaaac tgatagagaa
      601 agggcatgcc tacgccgcgc ccgacgggtc ggtctatttc gccgtcgaga gtttcaaggg
      661 ctacggcaaa ctgtcgaacc gcaaactcga cgaactgctc gcggggaccc ggttcgagcc
      721 cgaggcgggg aagcgcaatc cactcgattt cgcgctctgg aaatcggcaa aaccgggcga
      781 gccgtcgtgg gattcgccgt ggggcggcgg gcgcccgggc tggcacatcg agtgctcggc
      841 gatgtcgtcg aaatacctcg gcgattcgtt cgacatccac gccggcggcc gcgacctgat
      901 attcccccac cacgagaacg agatcgcgca gtcggaggcg gcatcgggca gaccgttcgt
      961 caaatactgg gtccacaacg ggttcctgac ggtcgaaggg gagaagatgt ccaagtcgct
     1021 cggcaacttc atcaccgtcg acgacgcgct cgaccagtat caccccgagg tcctgcggct
     1081 gttcatgctc tcgacccact accgcacccc gatagatttc tcctacgccg ggctcgccga
     1141 ggcggaacgg cgcgtctgct atttctacga aaccataaag aacatcaaga aactggcggg
     1201 ggatgccgcc gcgaagacga ccgaacgggc cgatgcgttc cgcgccgagt tccgcgagtc
     1261 gatggacgac gatttcaaca gccccaaggt gatggcggcg ctcttcggta tctgtaagtt
     1321 cctcaacgag tcggcggtca gcaaaaagac gcggccggcg cccgacgagg cggccggtta
     1381 cctcacggcg atcgccgaag cggggaaggt cttcgggctt ctgcaggccg acccggcgac
     1441 gatcatcgaa gcaataaaag atctccagct tgaacgggcg aagctgaccc gccgcgagat
     1501 agatgcgctc atcgccgagc gcaacgccca ccgcaaggag aaggatttcg ccgccgccga
     1561 cgcggtgcgc gcgaaactcg acgcgctggg catactcatc aaggacaccc ccgacgggac
     1621 cgagtggtcg ttcaagtaac gccgccccct ctcccctttc aagatttcat ccccccgcta
     1681 ttgctttttg accggtatcc gctaaaattt ccgcatgtgc ccgtcgaata tggacatatt
     1741 gtggtgaggt ggtcatcatg aactatcgcc gttccttgat cctcgcggcc ctcgcttccc
     1801 ttgcggcgct gttgagcgcc tgtgccgagc cgcaggatga ccgcgtcagt tgcgtcacct
     1861 ccttcgactg tccgctcggc tggttctgcg aggattcatg gtgcgttcag aaccccgacc
     1921 agagcgcggg gggcgacacg aagaacgaca tcgatacgag cgtcgatatc gacaccgcga
     1981 ccgccgagga cgataccctt cccgacaccg ccgaaggttc cgacctgaac gatgacggcg
     2041 tgatccccga cgaacagggg gacgacgcgg tgatcgtcga cgataccgac gagccgaccg
     2101 tcgaaggaga tgcgttgata cctgacgacg ataccggcgc gaccgacgac gggaccgata
     2161 ccgacaccgc gaccgacggg gacatcgtca ccgacaccgc gaccgatgcc gacgccgtgc
     2221 cccccaacac cggtgccacc tgctcggtgg cgggagattg cgtcggcggc gccacctgcg
     2281 gccagatatt caccggccag accacgagtt actgctacct gagttgtgca tggccgaacg
     2341 agagcatctg cgccggtacc gcgtggcccg aatgcgcggt catcagcagt tgggccctct
     2401 gcctcgccac ggcgacggta ggcgggaatt tcaccgcgct ggtcgactct ttccagcagg
     2461 gtaacaacat ctcgctgacc atcaacggca ccaaccacat ccagagcgga tgcgtagcgt
     2521 cactgagcgg ctcgacatgg atcctgcaat gttcgaaggt gctcgagagc acgccgaaca
     2581 ccatccaact cgacaccgtg ttctactggg acaccggcga acacaatacc gacggaacgg
     2641 tcgcgaacgc cgagggacgg gcctatcaga ccaccttcgt cggcaccacc aacgaggtgt
     2701 ccgagcgctg ggtacgcgcc gttttcaccg tcaacgacgg cacgctgacc ttgacgcagg
     2761 cgggcaccgc cgccggctcc acggtcgccg ggacgataaa cttcaccggt aacgcctacg
     2821 acgcccagtt gacggtccag taacgcccct gttgacaacc atttcttttg tactattatc
     2881 ccccccgaga cgataggaga cgccatgttc cgcaagatgc tcaagtcgaa actgcaccgc
     2941 ctgcgcatca ccaacgccga cctcatctat cagggcagca tcaccataga cgccgaactg
     3001 ctcgaacggg ccgatatccg atcctatgaa ttcgtggatg tctggaatgt gtccaacggc
     3061 gcccgtttcg agacctatgc cattccgggg gaacggggaa aacgcgaagt atgcgtcaac
     3121 ggcgcggcgg cgcggctcgt gaaccgcggc gacatcatca tcgtcgcgag ccaccgctac
     3181 ctcaccgagg aggaggcggg cgacctgacg ccgaccatcg tgttcgtcga cgagaacaac
     3241 catccccttt cggaggcacc gtgggcaaag aacaccgccg cttcaaacgg gccgagatag
     3301 cgatacgcat ccggctcaga gagtcggaca ccgatatcga ggcatccttc gccctgcgcg
     3361 acctcagcga aggcggcctg ttcgtccatt cgccgatact gtgggaaccg ggcgagaagt
     3421 tcgaattatc cttcatgttg ccgggctccg accgtgagat acacgcgacc ggaaa
//
LOCUS       DAOQUE010000299         3467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31030_length_3467_cov_4.263775, whole genome shotgun sequence.
ACCESSION   DAOQUE010000299 DAOQUE010000000
VERSION     DAOQUE010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31030_length_3467_cov_4.263775"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PKH10_09160"
     CDS             complement(<1..901)
                     /locus_tag="PKH10_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT28331.1"
                     /translation="MEKRQVLIVDDELSMRQFLEILLKKEGYEVVVAENGDRALEKMQ
                     KSEFAVVLMDYNMPEGIDGIDLLNRLRRLRPASQIVVITAYASTEQAIKAIELGALDY
                     VSKPFNVAEIKGIVRRAVQEYEGKARSSSGDTPSGEGSAGTVAVIAESPGMKKVLDYA
                     RQVAPTDSTVLITGESGAGKEIVARFIHNNSERRNAPWYPINCGAIPETLQESELFGY
                     EKGAFTGADKIKRGYFEVVGTGTLFLDEIGELSENMQVKLLRVLQDHIFMRVGGTETL
                     RAGARLIAATNRNLHKDVERGRFR"
     gene            complement(873..2474)
                     /locus_tag="PKH10_09165"
     CDS             complement(873..2474)
                     /locus_tag="PKH10_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT28332.1"
                     /translation="MDFFRAENFRKNLHSFFIIRFFFLIALFFAFFLLPFINERIELF
                     SFRNKLLLILVFFFTLVNFLSIVMGGRILNERRLRLFAYLQFFLEIFFWVLVAYLTGG
                     IESPYLYVVVITIMYSGFILDEKGALFTTVASFTLLLLMAFLIKSSLLPIVSMELVDL
                     YALSWVSFFSRLFVYLFFFSIAGLMATGMARAVEKVNRELMERELMTQELKAHFYAIF
                     SSLSIGFVITRNKKMMYANDFARRTCGALDRFIDKILGTPYTYLAWSEIAWEKRDLSY
                     SVQQYVDQQEVIVFSDVTELRLREEENRRREKMAALGQLTASIAHEIKNPLASLMGAS
                     EVIFANTQSVDEDSRRLMDIILREGDRVKRLLDNLFTSTEERRLTISAVPLKALIGDV
                     IRIFALSYPDVEITAELDEVTVDGDADRLREVCWNLLLNSVEAMKKKGRIMLRLEIER
                     PFALITLTDQGGGISPEYVDRIFDPFFSTKKRGTGLGLAVVFSVVKMHHGEITVRNVE
                     GGTEFRLRIPETQEIPSGKTTSTDR"
     gene            complement(2478..>3467)
                     /locus_tag="PKH10_09170"
     CDS             complement(2478..>3467)
                     /locus_tag="PKH10_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT28333.1"
                     /translation="HAVKTASAAEATVSQLEAANARLTEQVNAALGIQEATLSVEEAL
                     KEHPTVFTDLYVNLVAAGEVGGILDTILDRLAMFLEKNDKLKKRVKGAMSYPLVVLVA
                     TVGIVAVLLVWVIPTFAAMFISQGQELPGLTKMIVDLSDWFQANIILMAIIGAGAFVG
                     IKVLLKNKKIQYRMHKLALGAPVFGDLIKKTSVARFTRTLATLLSSGVPLVDGLQVVS
                     STSGNMVVEEGILFVRDRVMEGQDMTTPLVDVKIFPQMVVSMIGVGEATGAMDTMLSK
                     IADFYEEEVDVAVGALTSMLEPIMMVFIGGIVGTTMVAMYLPIFKMAGTVGGE"
BASE COUNT          737 a         1032 c          976 g          722 t
ORIGIN      
        1 cgcggaaccg accccgttcg acgtctttat ggagattgcg attggtcgcg gcgatgagtc
       61 gcgcgccggc gcggagggtc tcggtgccgc cgacccgcat gaatatgtga tcctgcagga
      121 cccgcaagag tttcacctgc atgttctccg acagctcccc tatctcgtcg aggaacagcg
      181 taccggtgcc gaccacctcg aaatatcccc gttttatctt gtccgcaccg gtgaaggcac
      241 ccttttcata accgaacagt tcgctctcct gcaaggtctc cggtatcgcg ccgcaattta
      301 tcgggtacca cggagcgttc cgccgttcgc tgttgttatg gatgaaacgg gcgacgatct
      361 ccttacccgc gccgctctcg ccggtgatga gcaccgtcga gtcggtcggc gcgacctgcc
      421 gcgcatagtc gagcaccttc ttcatacccg gactctcggc gatcacagcg accgtaccgg
      481 ccgatccctc tccgctgggc gtgtcgcccg aggacgaccg tgccttcccc tcgtattcct
      541 gcaccgcacg gcgcacgatg ccttttatct cggccacatt gaagggctta ctcacatagt
      601 cgagcgcacc cagttctatc gcctttatcg cctgctcggt cgaggcatag gcggtgatga
      661 cgacgatctg gctcgcgggg cggagacgcc gcaggcggtt cagcaggtct atgccatcga
      721 tcccctccgg catattgtag tccatgagga ccacggcgaa ttcacttttc tgcatctttt
      781 cgagggcgcg atcaccgttc tcggcgacca cgacctcgta cccctccttt ttcaggagga
      841 tctcgaggaa ctggcgcatg ctcagttcgt cgtcaacgat cagtacttgt cgtttttcca
      901 ctcggtatct cctgtgtttc gggtatccgc aggcggaact cggtaccacc ttccacattc
      961 cgcaccgtta tctcgccgtg atgcatcttc acgacgctga acaccaccgc gagccccaga
     1021 ccggtccccc gtttcttggt cgagaagaac ggatcgaata tcctgtcgac atactccggc
     1081 gatatccctc ctccctgatc ggtcagcgtt atgagggcga acggcctctc gatctcgagt
     1141 cgaagcatta tccggccttt cttcttcatc gcctcgacac tattgagcaa aaggttccag
     1201 cagacctcgc gcaggcggtc ggcatcgccg tccaccgtca cctcatcgag ttcggccgtt
     1261 atctcgacat cggggtagga aagcgcgaat atccgtatca catcgccgat aagcgccttc
     1321 aacggtaccg cggagatggt gaggcgccgc tcctcggtcg aagtgaacag attatcgagc
     1381 aaccgcttca cgcggtcacc ttcgcgcaat atgatatcca tgagccggcg gctgtcctcg
     1441 tcgacgctct gtgtgttcgc gaatatgacc tcgctcgctc ccatgagact tgcgagcggg
     1501 ttttttatct cgtgggctat cgaggcggtc agttgaccga gcgccgccat tttttcgcgg
     1561 cgtcggttct cctcctcgcg caggcgcagt tcggtgacat cggagaagac gatcacctcc
     1621 tgctgatcca catattgctg gaccgagtag gacagatcgc gtttttccca cgcgatctcc
     1681 gaccacgcga gataggtata aggcgttccc agtatcttat cgatgaatcg gtcgagagcc
     1741 ccgcaggtcc ggcgggcgaa gtcattcgcg tacatcatct tcttgttgcg ggttatcacg
     1801 aatcctatcg aaagcgatga gaatatggca tagaaatgcg ccttcagttc ctgcgtcatc
     1861 agttcccgtt ccatgagttc gcggttcacc ttctcgacgg ctcgcgccat gccggtggcc
     1921 atgagcccgg ctatcgaaaa gaagaaaaga tagacgaaca accgcgagaa gaacgatacc
     1981 caactcaacg cgtagagatc gacaagttcc atcgaaacga tggggagcag ggaagatttg
     2041 atgaggaagg ccatcagcag gaggagcgtg aaactcgcga ccgtggtgaa gagcgctccc
     2101 ttctcatcca gtatgaaacc agaatacatg atggtgatga cgacgacata cagatagggg
     2161 gactctatgc cgccggtcag ataggcgacc agcacccaga agaatatctc gaggaagaac
     2221 tggagatagg cgaagagacg gagacgccgc tcgttgagga tgcgcccccc catcacgatc
     2281 gaaaggaaat tcaccaatgt gaagaagaat acgagtatca gaagcagttt attgcggaaa
     2341 ctgaacagtt ctatccgttc gttgatgaac ggcaacagga aaaaggcgaa aaagagggct
     2401 atgaggaaaa agaaacggat gatgaagaac gaatggaggt tcttccggaa attctcggcg
     2461 cggaaaaagt ccatcgacta ttcgccgccc accgtaccgg ccatcttgaa tatcggcagg
     2521 tacatggcga ccatggtcgt accgacgatg ccgccgatga agaccatcat gatcggttca
     2581 agcatcgagg tgagggcgcc gacggcgaca tcgacctcct cctcgtagaa atcggctatc
     2641 ttggaaagca tcgtatccat ggcgccggtc gcctcgccga ccccgatcat cgaaacgacc
     2701 atctggggga atatcttgac atcgacgagc ggcgtcgtca tgtcctgacc ttccatgacc
     2761 cggtcgcgca cgaacaggat gccctcttcg accaccatgt tgcccgaggt cgaggaaacg
     2821 acctgcaggc cgtcgacgag cggcacgccg gaactgagaa gcgtggcaag cgtccgggtg
     2881 aaccgggcga ccgaggtctt cttgatgaga tcaccgaaca ccggcgctcc gagcgcgagt
     2941 ttatgcattc gatactgtat ctttttgttc ttgaggagca ccttgatccc aacgaacgcg
     3001 ccggcgccga tgatcgccat gaggataatg ttcgcctgga accagtcgga aaggtcgacg
     3061 atcatcttgg tgagtccggg cagttcctgc ccctgactga tgaacatggc ggcgaaggtc
     3121 gggatgaccc agaccagaag caccgcgacg atgccgacgg tagcgaccag aacgaccagc
     3181 ggataggaca tggcgccctt gacccgcttc ttgagtttgt cgttcttttc gaggaacatc
     3241 gccagacggt caaggatggt gtcgagtatg ccgccgacct caccggccgc cacgaggttg
     3301 acatagagat cggtgaagac cgtcgggtgc tccttgagcg cctcctcgac ggagagggtc
     3361 gcttcctgga tgccgagcgc cgcgttcacc tgctcggtca gccgagcgtt cgccgcctcg
     3421 agttgcgaga cggtcgcctc ggctgcgctg gcggtcttca ccgcgtg
//
LOCUS       DAOQUE010000300         3465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31060_length_3465_cov_4.971554, whole genome shotgun sequence.
ACCESSION   DAOQUE010000300 DAOQUE010000000
VERSION     DAOQUE010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31060_length_3465_cov_4.971554"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKH10_09175"
     CDS             complement(<1..249)
                     /locus_tag="PKH10_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28334.1"
                     /translation="MIKYLMTLVVAMLIVACGDEGTKEPAMGSKGGDCYGNGTCDGDL
                     VCLSNVCVDPTETPDKDTVVNDTTQPDDSVTDTEQPDVD"
     gene            complement(265..1305)
                     /locus_tag="PKH10_09180"
     CDS             complement(265..1305)
                     /locus_tag="PKH10_09180"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNT28335.1"
                     /translation="MTKYLMLLAAMPLLIACGDEGTKGLAMGSERGSCYGNGTCDGDL
                     VCLSNVCVDPTKTSDNDSTVTDTTQPDDSVTDTEQPDVDYGTGTPGEMVDIPAGEFQM
                     GCNSAVDAQCGDDENPYHAVTLSAYKIGKYEVTVGEYQKCVTNGACNNSGEYTHYCCT
                     NTDNSYCNYGAQGKGSHPMQCVTWYGAKAYCEWIGGRLPSEAEWEKAARGTDGRKYPW
                     GNTPEASCDYAVMDDSNAGGDGCGTGGSMPVGSKPNGVSPYGVHDMIGNVWEWVNDWY
                     GEDYYEITPTNNPAGPETGANRVLRGGSWLSRYDFTYNNVLLLRVSNRSNYRPDSSTY
                     YYSGFRCAMDAE"
     gene            complement(1505..2683)
                     /locus_tag="PKH10_09185"
     CDS             complement(1505..2683)
                     /locus_tag="PKH10_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HNT28336.1"
                     /translation="MTQGNGSKISDAVQLVMDYGFEGMEEAVRILLNEAMRLEREKVL
                     NARPYERTEARQGYANGYKPKKVKSLLGEMHLSVPQVRGEVEFYPTALEKGLRSERAL
                     KTAMAEMYIHGVSTRKVRDILEKMCGLEVSSTDVSRATKLLDEELEKWRNRPLGEVPY
                     LQMDARYEKVREGGSVVSGAVLVAVGVLADGRRSVLGVSVSLSEAEVHWRNFLQRLKE
                     RGIHGVKLITSDDCEGLKKALKTVFGGVPWVRCHVHLQRNAQAYVPRQDMREEVAQSI
                     REILTAPNLDEAERLLAKAVAKYEKKAPKLARWMVGAIPEGFAIFSVPHRLRRKLRST
                     NLVERLQKEIKRRTAVCTLFPNEESLLRLVSAILMEQSEEWETGSVYMALGAENDPQS
                     "
     gene            complement(2781..>3465)
                     /locus_tag="PKH10_09190"
     CDS             complement(2781..>3465)
                     /locus_tag="PKH10_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28337.1"
                     /translation="EGTFSLCCYRGQIPFANTTTFIVVAATLMITCCGAEAKSSSYKL
                     PADAETYAKEHNLKDTFNISDRLGYDNGEMTDMLYVFDDFCSKTYPTTQIYSAYNGIG
                     TIYHYIKLSDIKSPVDFMFKSFAVGADLPDFVFAFVNYDNHEVFIRQGLTATYAPPAP
                     YLTYDIYYDPIRYEDMSRDYVATLFSVFNYLHDKSEKYLKSNQPPAIILCHAKYNLHP
                     IESEKQKIV"
BASE COUNT          767 a          989 c          785 g          924 t
ORIGIN      
        1 atctacatcg ggctgttcgg tgtcggttac ggaatcgtcg ggttgcgtcg tgtcgttgac
       61 gaccgtgtcc ttgtccggcg tttcggtcgg atcaacgcac acattggaca gacacacgag
      121 atcaccgtcg caggtaccgt ttccgtaaca atcgccccct ttcgatccca tcgccggttc
      181 tttcgtcccc tcatcgccac aagcgactat caacattgcc accacaaggg tcataagata
      241 cttaatcata ggtccctccc ttttttattc agcatccatt gcgcaccgga acccggagta
      301 gtagtaggtg ctgctgtccg ggcggtagtt gctgcggttg gatacgcgca ggaggaggac
      361 attgttgtag gtgaagtcgt agcggctgag ccacgacccg ccgcgcaaca cgcggttagc
      421 accggtttct ggccccgcag ggttgtttgt aggcgttatt tcgtaataat cttcaccata
      481 ccaatcgttc acccattccc agacatttcc tatcatatcg tggacgccgt aaggcgatac
      541 accgttcggt ttgctcccca ccggcatgct gcctccggtg ccgcatccat cgcctccggc
      601 gttcgagtca tccatgacgg cataatcgca tgacgcttcc ggcgtattac cccacgggta
      661 tttccgtcca tcggtgcccc gcgccgcttt ctcccattcg gcctcgctgg gaagtcgtcc
      721 gcctatccat tcacagtaag ccttcgctcc gtaccatgtc acgcactgca tcgggtggct
      781 tcccttcccc tgcgcaccat agttgcaata ggaattatcg gtgttggtgc aacagtagtg
      841 tgtgtactcc ccgctgttgt tgcatgcccc gttggtgaca catttctggt attcgcccac
      901 ggtcacttcg tacttgccga tcttgtaggc gctgagtgtc acggcgtggt atgggttttc
      961 gtcatccccg cattgggcgt ccaccgctga attacacccc atctgaaact caccggcggg
     1021 gatatccacc atctcgcccg gcgtcccagt accgtaatct acatcgggct gttcggtgtc
     1081 ggttacggaa tcgtcgggtt gcgtcgtgtc ggtcacggtt gagtcgttgt cggaagtttt
     1141 ggtcgggtca acgcagacat tcgataagca gaccaaatca ccgtcgcagg tgccgttccc
     1201 gtagcaagaa cctctttcgg atcccatcgc caggcctttc gtcccctcgt cgccacaggc
     1261 gatcagcagg ggcatcgccg caaggagcat caggtatttg gtcacagtgt cctccctcta
     1321 tttattcagc atccttcgcg caccggcgac tcaagaggtc attctaggct aatagatgct
     1381 attaagttca aatatttttt tttcatccgc aacttttttt gcctcttcca taaacatggt
     1441 atatggcaca acaacaaatc tacctatagc tttttgtttg ataaagcaac aatttttctg
     1501 taaattagga ttgcgggtca ttctcagcac ctagcgccat ataaaccgaa ccggtctccc
     1561 attcttcgct ttgctccatg aggatcgcgc tcaccaacct aagcagagat tcctcgttgg
     1621 gaaagagcgt gcataccgcc gttcttcgct tgatctcctt ctgtagccgc tcaacgagat
     1681 tggtcgagcg cagtttcctg cggagccgat gcggcaccga gaagatggcg aagccttcag
     1741 ggatggcccc caccatccac cgggcaagtt tcggcgcttt tttctcgtac tttgccaccg
     1801 ctttcgccag aaggcgctcc gcctcgtcta ggttgggcgc cgtcagtatc tcgcggatgc
     1861 tttgagcgac ctcttcgcgc atatcctgtc tcgggacata ggcctgcgca ttccgctgaa
     1921 gatgcacatg gcaacgcacc cacggcacgc cgccgaaaac cgttttcagg gccttcttca
     1981 acccttcgca gtcgtcgctc gttatcaact taacgccgtg tatgcctcgc tccttgaggc
     2041 gttgcaggaa attgcgccag tgtacctccg cctcgctgag tgacacgctc acgccgagaa
     2101 cggaccgccg gccatccgcc agtacgccga cggccaccaa caccgcgccc gaaaccactg
     2161 agccgccttc ccgtaccttc tcataacgag cgtccatttg cagatagggc acttctccga
     2221 gcggtcggtt ccgccatttc tccaattctt catccagcag tttggtcgcc cgcgacacat
     2281 cggtgctgct gacctccagt ccgcacatct tctccaaaat gtcccggacc ttcctcgtcg
     2341 agacgccgtg aatgtacatc tccgccatcg ccgtcttgag ggctcgttca ctgcgcaggc
     2401 ccttttccaa ggcggtcgga tagaactcta cctcgccgcg cacttgcggc accgaaagat
     2461 gcatctcccc caacaggctt ttcactttct tcggcttgta tccgttcgca tatccttggc
     2521 gtgcctccgt ccgctcataa ggcctagcgt tgagtacctt ttcccgctca agccgcatcg
     2581 cctcgttcag aaggatccgt accgcctctt ccatcccttc aaagccgtaa tccataacta
     2641 actggacagc atcagaaatc ttactaccat tcccttgggt catgtcgcac ctccgttgtt
     2701 ggtactacga agatgctgat atgacccttc ttggcaattt cttgcctccc cggctcaaaa
     2761 accaatttac agaaacgatg ttacactatc ttttgttttt cagattcaat aggatgaaga
     2821 ttatatttag catgacaaag aattattgcg gggggttgat tggattttaa atatttttct
     2881 gatttatcat gtaaataatt gaacactgaa aaaagagtgg caacataatc tctgctcatg
     2941 tcctcatatc gtattggatc ataataaata tcataagtga ggtaaggtgc cgggggtgcg
     3001 tatgtagcag taagaccttg tctaataaaa acctcgtgat tatcataatt gacaaaagca
     3061 aatacaaaat ccggcaaatc agcacctacc gcaaaacttt taaacataaa atcaacaggg
     3121 gatttaatat cgcttaactt tatgtaatgg taaattgtgc caataccatt atatgcagag
     3181 taaatctgtg tggtgggata tgtttttgag cagaaatcat caaaaacata aagcatatct
     3241 gtcatttcgc cattatcata tcctaatcta tctgaaatgt tgaaagtatc tttcaaatta
     3301 tgttccttag cataagtttc tgcatctgca ggtaatttgt atgaagagga tttagcttct
     3361 gccccacaac atgtaatcat taaagtagca gcaactacaa taaatgtagt tgtgttagca
     3421 aagggaattt gtcccctata acagcaaagg gaaaatgtcc cctct
//
LOCUS       DAOQUE010000301         3461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31107_length_3461_cov_3.918379, whole genome shotgun sequence.
ACCESSION   DAOQUE010000301 DAOQUE010000000
VERSION     DAOQUE010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31107_length_3461_cov_3.918379"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="PKH10_09195"
     CDS             <1..1398
                     /locus_tag="PKH10_09195"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNT28338.1"
                     /translation="IELGQALTADRILTGIVTYFGKQYTLTAELIDLAKEATIKTAKA
                     EFDGSEEGLAEAIKSIAAQIADKKAGGSSFKEGKIGEQVEDWDIGQGEETIVKFESEP
                     TGATVRVDGKLLCQQTPCSKMLTQGKHAITMELENYQAKTKTGTVKKGTQIKWQMEPD
                     FGYLSVESKPAGIEVTLDGKSIGKTPLEKVTLSPGPHQVETKDNCYYQTGEKFTANRG
                     ESKKIRLDVKPKEAGIKVTAQDKNGNDIEATVIVDGKEIGTTPGKWKIPVCAKQLLVR
                     NKNGEYKKILNPREKELVTINAILEKSREYDIQDTVVVNNKTGLMWQKEISSHTMNQD
                     NAYAYCQDLSLEGYSDWRLPTISELRTLIKGCPKTEPGGMCKLTDDCLSVIKCHSFKA
                     CGCEVAKGPGENGFYWQKDVWSYDGDQHGHFWSSSVLSDFPAGGWDVCFEEGAVMNYS
                     DRKANFYVRCVRSNE"
     gene            1438..1953
                     /locus_tag="PKH10_09200"
     CDS             1438..1953
                     /locus_tag="PKH10_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28339.1"
                     /translation="MQNINDDRHCLLDLKKEPILGKGIAIVYVYFLIKEFISYPKEKS
                     NILYIGEALRHKEPTGVRLGQHITHKKEEGSDTGSNLVLSQYFYEDWHLGLAIFSTTP
                     KARKRDERNLIYAHISLYGAPPIAQGKLPYDGKKGKGRNRTKHIFDYIANNKKAIANA
                     SKILEDIVVEN"
     gene            1965..2753
                     /locus_tag="PKH10_09205"
     CDS             1965..2753
                     /locus_tag="PKH10_09205"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNT28340.1"
                     /translation="MSNIIFYYTGTGNSLHAAKRAADALGGAELIHMGRKRDHGYKAL
                     NADAVGFVFPVYAWGPPVAVEKFLKHLDIAKPAYTFTILTHGGAPADTLPMWDDMLGG
                     KGFRNDAGWALRAVDNYIPFYNPPAPEKQADINTRLDNNLDTVCERIKNREKNELKGS
                     LPGKFARYLIHPMWASHVNSSDKNWKVSEKCTGCGICAKVCPVGNITMSAEKRPGWNH
                     ACEQCMACFHWCPEHAITWGKKGADYNHYHHPGVKVTDLFPDDK"
     gene            2750..>3461
                     /locus_tag="PKH10_09210"
     CDS             2750..>3461
                     /locus_tag="PKH10_09210"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNT28341.1"
                     /translation="MKFNPVDPRRLTVLKPGAPGSGPVLYWMSRDQRVSDNWALIHAQ
                     NEAVKKGRPLAVVFCLAPRFGEASRRHYDFMLGGLREVEERLRRFDIAFRLFIGDPVV
                     TLPRELTKLDPALVVCDFDPLREKIRWRAAVTAALSCPVVEVDAHNIVPARIASPKKE
                     YAAATFRPKLNRLLAEFLLPFPKLKKHPVPFTATPADRDTATLNAASAGPALTMAPGE
                     KAAWKRLERFLKSGLARYG"
BASE COUNT          935 a          964 c          925 g          637 t
ORIGIN      
        1 attgagttgg gacaggcgct cacggccgac cggatactga ccggcatcgt cacctacttc
       61 ggaaaacagt acaccctgac ggcagaactc atcgaccttg ccaaagaggc gaccataaaa
      121 acggcaaagg ccgagttcga cggttccgag gaaggacttg ccgaggcgat aaagagcatc
      181 gcggcgcaga tcgcagacaa aaaagcgggc ggcagctcgt tcaaggaagg gaagataggc
      241 gaacaggtag aagattggga catcggccaa ggcgaagaga ccatcgtcaa gtttgagtcg
      301 gagccgaccg gggcaaccgt tcgcgttgac ggcaagttat tgtgtcagca gacgccctgc
      361 tcgaagatgc tgacgcaggg gaagcatgcg ataacgatgg aattggagaa ctatcaggca
      421 aagacgaaga ccggaaccgt taagaaaggg acgcagataa aatggcaaat ggaaccggac
      481 ttcggctacc tctccgttga gtcgaaacca gcggggatcg aagtaacgct cgacggcaag
      541 agcatcggca agacgccgct tgagaaggtc acgctctccc ccggacctca tcaggtcgaa
      601 acgaaagata actgctacta tcaaaccggc gagaagttca ccgcgaaccg tggcgaatcg
      661 aagaaaatcc gcttggatgt caagccgaag gaagccggca tcaaggtcac ggcccaagat
      721 aaaaacggga acgacatcga agcaacggtt attgtagacg gcaaagagat tggcaccaca
      781 ccgggaaagt ggaaaatacc tgtgtgcgcc aagcaactat tggtaagaaa taaaaatggg
      841 gaatataaaa agattctcaa cccaagagaa aaagagctgg tcacaataaa tgccatcctg
      901 gaaaaatcaa gggagtatga catacaagac accgtcgtcg taaataacaa gaccggttta
      961 atgtggcaaa aagaaatatc gagtcatact atgaatcaag ataacgctta tgcttactgt
     1021 caggatcttt cgttagaagg ttactctgac tggcgcctac ctactatttc agaactcagg
     1081 acactcataa agggatgccc caaaaccgaa cccggcggca tgtgcaaact caccgatgat
     1141 tgtctttcag tcatcaagtg ccatagtttt aaagcgtgtg gctgcgaagt tgcaaaaggg
     1201 cccggagaga acggttttta ttggcaaaag gacgtgtgga gttacgatgg cgatcaacac
     1261 ggacattttt ggtcatcatc ggtgctatca gattttcctg ccggtggttg ggatgtgtgt
     1321 tttgaagaag gcgccgtgat gaattacagt gacagaaaag ctaattttta tgtgcgttgt
     1381 gttaggagca acgaataacc ttttgtggga gagacttctt tgaaactagt tcattacatg
     1441 cagaacatca atgatgatag acattgcctt ttggatctca aaaaagaacc tatcctcggc
     1501 aaaggcattg ctatcgttta tgtatatttc ctgataaaag agtttatttc ttatcccaaa
     1561 gagaaaagca atattttata tatcggcgaa gcattacgac acaaagagcc caccggggtt
     1621 agattggggc agcacataac acacaagaaa gaagaagggt ctgacacggg aagcaacctt
     1681 gttttatctc agtattttta tgaagactgg cacctggggc tagctatttt cagcacgacg
     1741 ccaaaggcta gaaaaaggga tgagcggaat ctcatctacg cacatatatc actatacggt
     1801 gcccccccta tcgcacaagg aaaacttcct tacgatggaa aaaaaggcaa aggaagaaat
     1861 cgtactaaac atatttttga ttatattgct aacaacaaaa aggcgatagc taatgcatca
     1921 aagattttag aggatattgt cgtagaaaac taggagccaa ccccatgagc aacatcatct
     1981 tctattacac cggcaccggc aacagcctgc acgccgcgaa acgggccgcc gacgcgctgg
     2041 gcggcgccga actcatccac atgggccgca aacgcgacca cggctacaaa gcgctgaacg
     2101 ccgacgcggt cggcttcgtc ttccccgtct acgcatgggg cccgccggtc gccgtcgaga
     2161 agttcctcaa acacctcgac atcgccaagc ccgcctacac cttcaccatc ctcacccacg
     2221 gcggcgcccc cgccgacacc ctcccgatgt gggacgacat gctgggcggc aaaggattcc
     2281 gcaacgacgc cggttgggcg ctccgcgcgg tcgacaacta catccccttc tacaatcccc
     2341 ccgcccccga gaagcaggcc gacatcaaca cgcgactcga caacaatctc gacaccgtct
     2401 gtgaacggat aaagaaccgc gagaagaacg aactcaaagg ctcgcttccc ggcaagttcg
     2461 cccgctacct catccacccg atgtgggcct cccatgtcaa ttcgagcgac aagaactgga
     2521 aggtgtcgga gaaatgcacc ggctgcggca tctgcgcgaa ggtctgcccc gtcggcaaca
     2581 tcacgatgag cgccgagaaa cggcccggct ggaatcacgc ctgcgagcag tgcatggcct
     2641 gtttccactg gtgtcccgaa catgcgatca cgtggggcaa gaaaggggcc gactacaacc
     2701 actaccatca ccccggcgtc aaggtcaccg atctgttccc cgacgataaa tgaaattcaa
     2761 cccggtcgat ccgcgccgtc tgacggtgct gaaaccgggc gcgccgggga gcggccccgt
     2821 cctctactgg atgagccgcg accagcgggt gagcgacaac tgggcgctta tccacgcgca
     2881 gaacgaggcg gtgaagaagg ggcgtccgct cgcggtcgtg ttctgtctcg cgcctcgctt
     2941 cggtgaggca tcgcgacggc actacgactt catgctcggc ggcctgcgcg aggtcgaaga
     3001 gcggcttcgc cgtttcgaca ttgccttccg cctctttatc ggcgatccgg tggtcaccct
     3061 gccgcgcgaa ctgacgaaac tcgacccggc gctggtggtc tgcgacttcg atccgctccg
     3121 cgaaaagatc cgctggcgcg ccgcggtcac cgccgcgctg agttgtcccg tggtcgaggt
     3181 cgacgcccac aacatcgtcc cggcccgcat cgcctcaccg aaaaaggaat atgccgccgc
     3241 caccttccgg ccgaagttga accggctcct cgccgaattc ctcctcccct tcccgaaact
     3301 caagaaacac cccgtcccgt tcaccgccac cccggccgac cgggacaccg ccaccctcaa
     3361 cgcggcatcg gccggaccgg cactgacaat ggcaccgggt gagaaagcgg cgtggaagcg
     3421 actcgaacgg ttcctgaaaa gcggccttgc ccgttacggc g
//
LOCUS       DAOQUE010000302         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31126_length_3460_cov_3.743906, whole genome shotgun sequence.
ACCESSION   DAOQUE010000302 DAOQUE010000000
VERSION     DAOQUE010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31126_length_3460_cov_3.743906"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKH10_09215"
     CDS             <1..390
                     /locus_tag="PKH10_09215"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT28342.1"
                     /translation="RGTDGRKYPWGNEPAVSCDYAVMDDANVGGKGCGTGGTMPVGSK
                     PNGVSPYGAYDMIGNVWEWVNDWYSSDYYASSPTNDPSGPEIGDGRVLRGGSWRNGYD
                     YYLFRASCRNYDGAVSSFNNYGFRCAK"
     gene            583..1515
                     /gene="meaB"
                     /locus_tag="PKH10_09220"
     CDS             583..1515
                     /gene="meaB"
                     /locus_tag="PKH10_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNT28343.1"
                     /translation="MTKKKLDHRALARQISEIENDRDAAETTLAKLYRDPDTVQVIGI
                     TGPPGAGKSTLADCVIGKLREAGKTVAVVAVDPSSPFTGGAILGDRIRMSAHADDPGV
                     FIRSMGSRGSLGGLAHATFDVVNLLRSSGFDAVIVETVGVGQSEIDVLAVADTVVLTL
                     VPGLGDDVQALKAGIMEIADLFVINKSDRDGKERLVAEIRMILELNRDRFDWVPPICE
                     TVATEQRGIDGLMEQVALHAAYVRDHRDAVRRPKLARQLEKMTADKIGRDILAFLGKD
                     DRFAAWMDAIMAGKRNPYQVLQEIGNYISVRKEA"
     gene            1517..1921
                     /gene="mce"
                     /locus_tag="PKH10_09225"
     CDS             1517..1921
                     /gene="mce"
                     /locus_tag="PKH10_09225"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNT28344.1"
                     /translation="MKKINHIGIAVEKIDDYIAFYRDTLGLPVEGAEMVEEQKVKVAF
                     LTIGDTRIELLEPTSPESPIAKFIEKKGAGVHHLAFEVDDIAAELAKMKEKGVKLIDE
                     TPRGGAHHTKIAFIHPKESKGVLVELTESGSH"
     gene            1941..>3460
                     /locus_tag="PKH10_09230"
     CDS             1941..>3460
                     /locus_tag="PKH10_09230"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNT28345.1"
                     /translation="MATIEEKLAQLRAKKEQAKLGGGEKRIEDQHKKGKYTSRERFAK
                     LLDPGSFEELDCLRSDRKEGSSLGDVVVIGYGTIDGRKVFVFGQDFTVSGGSLGEVVA
                     AKICKVMDLSLKTGAPCIGLNDSGGARIQEGIHSLYGYGEIFKRNVLASGVVPQLSAI
                     YGPCAGGAVYSPALTDFVFMTNTNSYMFLTGPNVVKTVTHENVTVDQLGGAQVHMTKS
                     GVAHFSHDTEDESIAAIRELFSYLPSNNMEEPPRVACTDPVDRMDEELNTIVPLNPNK
                     GYNVKDVIRMIFDDGKFFEVQPLYAMSIVVGFARLNGRTVGIVANQPNVLAGVLDVNS
                     SMKGSRFIRFCDCFNIPLITLVDTPGFMPGTVQEYGGVIKHGAKMLYAYSEATVPKLA
                     VVMRKAYGGAYIVMSSKHLRGDMNYAWPTAQIAVMGAKGAVEILKRKELDAAADKAKF
                     IAEQEAEYAEKIENPYIAAQHGFIDDIFEPKTTRARLAKALEMMSTKRDDLPPKKHG"
BASE COUNT          755 a         1010 c         1080 g          615 t
ORIGIN      
        1 cgaggcaccg acggacggaa atacccgtgg ggcaacgaac cggccgtttc gtgcgattat
       61 gccgtcatgg atgatgccaa tgtcggtggc aagggctgtg gcaccggtgg aacgatgccg
      121 gtcggaagca aaccgaacgg cgtatcgccc tacggcgcct acgacatgat aggtaatgtg
      181 tgggaatggg tgaacgactg gtacagctca gattactacg cctcatcgcc tacaaacgac
      241 ccctcggggc cagaaatcgg tgatggccgc gtgttgcgcg gcgggtcgtg gcgcaacggc
      301 tacgactact acctctttcg cgcgtcctgc cgcaactacg acggcgcggt cagcagcttc
      361 aacaactacg ggttccggtg cgcgaagtga tctcccgtcc ccctgtcaag ggggatttga
      421 agggggtttt ctccaatcct gacgagttga aaacccaccc agccctccct tgacagggcg
      481 ggatgacgga tgacgggcgt cctcaccgaa aaattgactt agccgcgggg gcttattata
      541 aggtagcggt ctttgttttc ttttcgttag gttcgcgcgg gcgtgacaaa aaagaagttg
      601 gatcatcggg cactcgcccg ccagatttcc gaaatagaga acgaccgcga tgcggccgaa
      661 acgacgctcg ccaaactcta ccgcgacccc gatactgtgc aggtcatcgg catcaccggc
      721 cctcccggcg cggggaaaag cacgctggcc gactgcgtca tcgggaaact gcgcgaagcg
      781 gggaagacgg tcgccgtcgt ggcggtcgac ccgagttcgc ccttcaccgg cggcgcgata
      841 ctcggtgacc gcatccgcat gtcggcccac gccgacgatc ccggtgtctt catccgcagc
      901 atggggagcc gcgggagtct cggcgggctc gcccacgcca cctttgatgt ggtgaacctg
      961 ctccgttctt ccggattcga cgcggtcatc gtcgagacgg tcggggtggg gcagagcgag
     1021 atcgatgtcc ttgcggtggc cgacacggtg gtgctgacgc tcgtccccgg gctcggcgac
     1081 gatgtgcagg ccttgaaggc gggcatcatg gagatcgccg acctcttcgt catcaacaag
     1141 tcagaccgcg acggcaagga acggcttgtt gccgagatac ggatgatact ggaactcaac
     1201 cgcgaccgtt tcgattgggt gccgcccatc tgcgaaacgg tggcgaccga acagcgcggc
     1261 atcgacggtc tgatggaaca ggtcgcgctt catgccgcct atgtccgtga ccatcgcgac
     1321 gcggtgcggc gaccgaaact ggcccggcaa ctggaaaaga tgaccgccga caagatcggg
     1381 cgcgatatcc ttgcttttct cggaaaggac gataggttcg ccgcatggat ggacgcgatc
     1441 atggccggga aacggaaccc ctatcaggtg cttcaggaga tcggcaacta tatttcggtt
     1501 cgcaaggagg cataacatga agaagatcaa tcacatcggc atcgctgtcg agaagatcga
     1561 cgactacatc gccttttatc gcgacaccct cggacttcct gttgaaggag ccgaaatggt
     1621 cgaggagcag aaggtcaagg tggccttcct gaccatcggc gacacgcgga tcgaactgct
     1681 ggaaccgaca tcgcccgaaa gccccatcgc aaagttcatc gaaaaaaagg gggcgggggt
     1741 ccaccatctc gctttcgagg tggacgatat cgcggccgaa ctggccaaga tgaaggagaa
     1801 gggggtgaaa ctcatcgacg agacgccgcg cggcggcgcc catcatacca agatcgcctt
     1861 catccatccc aaggaatcga aaggtgttct ggtcgaactg accgagtcgg gaagtcacta
     1921 aaacataggg agttacgcgt atggcgacga tcgaagagaa actggcacag ttgcgggcca
     1981 agaaggagca ggcgaaactc ggcggcggcg agaagcgcat cgaggatcaa cacaagaaag
     2041 ggaaatacac ctcacgcgag cggttcgcaa aactgctcga tccgggcagt ttcgaggaac
     2101 tcgattgtct gcgctccgat cgtaaggaag ggagttcgct cggagacgtg gtcgtgatcg
     2161 gttatggcac gatcgacggc cgcaaggtct tcgtcttcgg gcaggacttc accgtgagcg
     2221 gcgggtcgct gggtgaggtg gtcgcggcga agatctgcaa ggtgatggat ctgtcgctca
     2281 agaccggtgc gccgtgcatc gggctcaacg attcgggcgg cgcccgcatt caggaaggta
     2341 tccattcgct ctacgggtac ggcgagatat tcaagcgcaa tgtcctcgcg tcgggcgtcg
     2401 tgccgcaact gtcggcgatc tatggcccct gcgccggcgg cgcggtctat tcgcccgccc
     2461 ttaccgattt cgtcttcatg accaacacca attcctacat gttcctgacc ggtcccaatg
     2521 tcgtcaagac ggtcacgcac gagaatgtga cggtcgatca actcggcggc gcgcaggtcc
     2581 acatgaccaa atcaggcgtc gcccatttct cgcacgacac cgaggatgaa tcgatcgcgg
     2641 cgatacggga actgttctcc tacctgccct cgaacaacat ggaggagccg ccacgcgtcg
     2701 cgtgcactga tccggtggac cggatggacg aagagctcaa caccatcgtg ccgctcaacc
     2761 cgaacaaggg ctacaatgtg aaagatgtga tccggatgat attcgacgac ggcaagttct
     2821 ttgaggtcca gccgctctat gcgatgagca tcgtcgtcgg cttcgcgcgg ctcaacggcc
     2881 gcaccgtggg catcgtcgcg aaccagccca atgtgctggc gggcgtgctc gatgtcaact
     2941 cctcgatgaa ggggtcccgg ttcatcaggt tctgcgactg tttcaacatc ccgctcatta
     3001 cgctcgtcga cactcccggc ttcatgcccg gcacggtgca ggagtacggc ggggtcatca
     3061 agcacggagc gaagatgctc tatgcctaca gcgaggccac ggtgccgaaa ctcgcggtgg
     3121 tcatgcgcaa ggcctacggc ggcgcctata tcgtcatgag ttcgaagcac ctgcgcggcg
     3181 acatgaatta tgcgtggccg acggcgcaga tcgccgtcat gggcgcaaaa ggggcggtcg
     3241 agatactcaa gcgcaaggaa ctcgacgcgg ccgccgacaa ggcgaagttc atcgccgaac
     3301 aggaggccga atacgccgag aagatcgaga atccctacat cgccgcccag cacggcttca
     3361 tcgacgacat attcgaaccg aagacgaccc gcgcgcggct cgccaaggcg ctcgagatga
     3421 tgagcaccaa acgggacgat ctgccgccga agaaacacgg
//
LOCUS       DAOQUE010000303         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31236_length_3451_cov_3.959364, whole genome shotgun sequence.
ACCESSION   DAOQUE010000303 DAOQUE010000000
VERSION     DAOQUE010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31236_length_3451_cov_3.959364"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKH10_09235"
     CDS             complement(<1..815)
                     /locus_tag="PKH10_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT28346.1"
                     /translation="MKHRILLIDDEVALLGTMRQILEKQGYDIITAHSGIEAMGIVKR
                     EIENLSLVLTDLLMPGGFDGIDLMEAVHQLDADIPVIVITAYGNVETAVKAMQKGAFN
                     FLTKPVNYKVLLQQIEKAAETLSLREENKRLRREIASLQEDRYRIVHASEKMRRLLES
                     AVEIAKTDETVLITGESGTGKELVARHILNESPRARRPFVAFNAAAVHPNLIESELFG
                     FKKGAFTGADRNSPGYVGAAEGGTLFIDEIGEMPTALQPKLLRFMQEREYPSLL"
     gene            971..1960
                     /locus_tag="PKH10_09240"
     CDS             971..1960
                     /locus_tag="PKH10_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28347.1"
                     /translation="MRRVLFMVLVLTGIVAGCGKNAEERYTLTLDLRFDAAEATDCTS
                     YQADKFVVTLYTDDFKTRKTFEFTCAADMRKPTLELAAGTYYLTVELLDGVYINSWGS
                     GQVSLIGDKTLAVDMNQYAGGIDIEWSSSLCDKLDIAVLSVSAITGGSPVSAIIWGQE
                     TTVTDLAIACHAERLSLNNIPSGPYAVTISGRRAEDAPRTRATATITVTLPAGQEKTL
                     TIDDKNLLLEVSDIKVSWEFDSKSIESCADAGVATIRASATGESDTFSEETACETEGT
                     LTVYDLPPGDYTITADGLDATGKVTYTGHKLLTVEPGKIGVEAYAETIYLKQE"
     gene            1978..2370
                     /locus_tag="PKH10_09245"
     CDS             1978..2370
                     /locus_tag="PKH10_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNT28348.1"
                     /translation="MLTKRQIDHFRKRLLAELKEIEERNKEKVEELEQETNDPSDKFH
                     GDEADQANFSEERSRLLRLRDRDRKLINKLHETLAKIDGGTYGICEACGTEIGAERLE
                     MRPVAGLCIECKQKQEEREERDKALKRR"
     gene            2376..3230
                     /locus_tag="PKH10_09250"
     CDS             2376..3230
                     /locus_tag="PKH10_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT28349.1"
                     /translation="MLSHAIIPAAGLATRFLPASKSVPKESFPLYDRPVIQHIAEEAA
                     QAGIHNIVLVTSEGKEAITDHFDRINPKFLQGKLPEDIARELDRLEELAEVIAVRQKS
                     QKGLGHAVLRGMTAVPTDPFAVMLPDMFLVSRSPDNVMVRMKRLYEQHDRPVLALMRV
                     PDEDRKRYGMAEGEERNGLFEIRRLVEKPGVDGTASNLAIVGRYLLPGAIGPILEKTT
                     PGAKGEIQLTDALNTLCGKQPMLGLVLRDDDLVFDAGDKDGYAYANAYLAAKRIPGFL
                     DTVRRMIS"
     gene            3238..>3451
                     /locus_tag="PKH10_09255"
     CDS             3238..>3451
                     /locus_tag="PKH10_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28350.1"
                     /translation="MHHPLLIVLLLAFFITPTQMVQQMLRNTVVDLPVVELAGETGDE
                     PPAPVTLKYDRAKDEVTLTVAQAPSVM"
BASE COUNT          703 a         1125 c         1035 g          588 t
ORIGIN      
        1 agtaacgacg gatattcccg ttcctgcatg aaccgcagga gtttcggctg aagcgccgtt
       61 ggcatctcgc cgatctcgtc gatgaagagc gtccccccct cggccgcgcc gacatagccg
      121 ggcgagttgc ggtcggcccc ggtgaaggca cccttcttga agccgaacag ttccgactcg
      181 atgaggttcg ggtggaccgc cgccgcgttg aacgcgacga acggacgccg ggcgcgcggg
      241 ctttcgttga gtatgtgacg ggcgacgagt tccttgccgg tccccgattc gccggtgatg
      301 agcaccgtct cgtcggtctt cgcgatctcg accgccgact cgaggagccg ccgcatcttt
      361 tctgacgcat ggacgatgcg gtaccggtcc tcctgcaacg acgctatctc gcgccggagc
      421 cgcttgttct cctcgcgcag ggagagcgtt tcggccgctt tctctatctg ttggaggagc
      481 accttgtagt tgaccggctt ggtcaggaag ttgaaggcgc ccttctgcat cgccttgacc
      541 gccgtctcga cattgccgta ggccgttatc acgatgaccg ggatgtcggc gtccaactgg
      601 tgcaccgcct ccatgaggtc gatgccgtca aagccgccgg gcatgagaag atcggtgaga
      661 acgagcgaca gattctctat ctcgcgcttg acgatcccca tcgcctcgat gcccgaatgg
      721 gcggtgatga tgtcgtaccc ctgtttctcc agtatctggc gcatcgtgcc gagcagggcg
      781 acctcgtcgt cgataagcaa tatgcggtgt ttcataacct ctccatatcc ctccgaattc
      841 gaccgattgt accgaaccgg acgaagagcg caaggtattt ctctggtatt tcttgaaaaa
      901 ataagggcgg cccggtataa tcggtccgat tgtccgatcc atgaaccgtc ctttgcgagg
      961 ggagaagccg atgcgtcgag ttcttttcat ggtccttgtg ctcacgggta tcgtcgcagg
     1021 gtgcggcaag aacgccgagg agcgctacac gctcaccctc gatctccgct tcgacgccgc
     1081 cgaggcgacc gactgcacca gttatcaggc cgacaagttc gtcgtcaccc tgtacaccga
     1141 cgatttcaag acccgtaaga ccttcgagtt cacctgcgcc gccgacatgc gcaagccgac
     1201 gctggaactt gcggcgggga cctattatct cacggtcgaa ctgctggacg gcgtctacat
     1261 aaacagttgg ggctccggac aggtctcgct gatcggcgac aagacccttg ccgtggacat
     1321 gaaccagtac gccggcggca tagacatcga atggtcttct tcgctctgcg acaaactcga
     1381 catcgcggtc ctttccgttt cggcgatcac cggcggctcc ccggtctccg cgatcatctg
     1441 ggggcaggaa acgacggtga ccgatcttgc catcgcctgc cacgccgaac ggctctcgct
     1501 caacaacatc ccctccggcc cctacgcggt cacgataagc ggccgtcgcg ccgaggacgc
     1561 gccgagaacg cgcgccacgg cgacgataac ggtgacgctc cctgcgggac aggagaagac
     1621 cctgacgatc gacgacaaga acctcctgct cgaggtgtcc gacatcaaag tgtcgtggga
     1681 attcgacagc aagagcatcg agtcgtgtgc cgatgccggc gtagcgacca tccgcgcctc
     1741 ggccaccgga gagagcgaca ccttctcgga ggaaacggcc tgcgagaccg aagggaccct
     1801 gacggtctac gacctcccgc cgggcgacta caccatcacc gccgacgggc tcgacgcgac
     1861 cgggaaggtc acctacaccg gacacaaact gctgaccgtc gagccgggga agatcggcgt
     1921 tgaggcctat gccgagacca tctacctgaa acaggaatga cctgatagga gaacgccatg
     1981 cttaccaaaa gacagatcga ccacttccgc aagcgcctgc tcgccgaact caaggagatc
     2041 gaggagcgca acaaggaaaa ggtcgaggaa ttggagcagg agaccaacga cccgtcggac
     2101 aagttccacg gcgacgaggc cgatcaggcc aacttctcgg aggaacggag ccgcctgctg
     2161 cgtctgcgcg atcgtgaccg caaactcatc aacaaactcc acgaaacgct cgcgaagata
     2221 gacggcggca cttacggcat ctgcgaagcg tgcggcaccg agatcggcgc cgagcggctc
     2281 gagatgcgcc cggtcgccgg cctctgcatc gagtgcaagc agaaacagga ggagcgcgag
     2341 gagcgggaca aggcgctcaa gcggagatag tcccgatgct ctcgcacgcc atcatcccgg
     2401 ccgccggact cgccacccgg ttcctgcccg cctcgaagtc ggtccccaag gaatccttcc
     2461 ccctgtacga ccgccccgtc atacagcaca tcgccgaaga ggcggctcag gcgggcatcc
     2521 acaacatcgt gctcgtcacc tcggaaggca aggaggcgat caccgaccat ttcgatcgca
     2581 tcaaccccaa attcttgcag ggaaaactgc ccgaggacat cgcgcgggag ctcgatcggc
     2641 tcgaggaact cgccgaggtc atcgcggtgc ggcagaaaag ccagaaaggg ctcggccacg
     2701 ccgtcctgcg cggcatgacc gccgtcccga ccgacccgtt cgcggtgatg ctccccgaca
     2761 tgttcctcgt gtcgcgttca cccgacaacg tgatggtgcg gatgaaacgg ctgtacgaac
     2821 agcacgaccg cccggtgctc gcgctcatgc gggtccccga cgaggaccgc aaacggtacg
     2881 gcatggccga aggcgaggag cgcaacggcc ttttcgagat acggcggctg gtcgagaaac
     2941 cgggcgtgga cggcaccgcg tctaacctcg ccatcgtcgg tcgctacctg ctccccggcg
     3001 cgatcggccc gatactggaa aaaacgaccc ccggagcgaa gggggagata caactcaccg
     3061 acgcgctcaa cacgctgtgc ggcaaacagc cgatgctggg actcgtcctg cgcgacgacg
     3121 atctggtgtt cgacgccggc gacaaggacg gctacgccta cgcgaatgcc tatcttgccg
     3181 cgaaacggat accgggattt ctcgacaccg tgcgccggat gatctcgtag ggacgccatg
     3241 catcatccgc tcctcatagt tctcctgctc gccttcttca tcaccccgac gcagatggtc
     3301 caacagatgc tccgcaacac cgtggtcgat ctccccgtcg tcgaactggc gggcgagacg
     3361 ggcgacgagc cgcccgcgcc ggtgacgctc aagtacgacc gcgcgaagga cgaggtgacg
     3421 ctgaccgtcg cgcaggcccc gtcggtcatg a
//
LOCUS       DAOQUE010000304         3446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31309_length_3446_cov_3.163669, whole genome shotgun sequence.
ACCESSION   DAOQUE010000304 DAOQUE010000000
VERSION     DAOQUE010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31309_length_3446_cov_3.163669"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PKH10_09260"
     CDS             <1..758
                     /locus_tag="PKH10_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNT28351.1"
                     /translation="LKTTADDKTLAAIADESQGDLRIALSILESAFYLSESKEIAWET
                     ARKVLLNPDKYDKKGDYHYRIISAFIKSLRGSDPDAALYYLVRMLDAGDDPLFLLRRM
                     IIFASEDIGNADPRALELAVAALHAFRHIGLPEGELTLAQCVTYLATAPKSNASYMAI
                     KKAKDFVRDHPDLKPPQYLVNSTSLAPEEEEGDYKYPHDFPGHWVDVRYFPEGADPQR
                     FYDPTDIGYEGKLKTYWDKLKKAVREGKKMPVE"
     gene            complement(762..>3446)
                     /locus_tag="PKH10_09265"
     CDS             complement(762..>3446)
                     /locus_tag="PKH10_09265"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28352.1"
                     /translation="GKSAPQDNASWPDWDLAGEEPDDDETGEKDESDIKDESDELTDG
                     DDPLPDMSDLPDDLLDDADAPIPDDDTDIDEIVTTASISWNGITAWVTATVDTYRTYE
                     LSTDNTLRDNYPPDKKVTIVEEPGQMRLRSGHLMFDALFALALQEARQNSVSEITDGS
                     FNGGQPMACDCFVTGEKWPYVWTRDTAYAAYLGLALVDPVRTMNSLLFKLSDKKATLG
                     GGAPQIVQDTGSGGSWPVSTDRVVWALGAYETLKYLSDADRAAFLTDAYAALKNTVET
                     DRSVAYDPHDGLYRGETSFLDWREQTYPAWTANDTVHIGMSKSLSTNQLHYRALRIAA
                     ELAFMAGESADQTRYTAWTDDLRAAIAARFAPGTTQTLMSAMKLTELNDAPLPRHDLL
                     ATALLAQWSDTIFDTGTTVGQIAFWPHTDAGAPVIWPQQPWTPIYHNRAIWPFVTALY
                     AQAARNTANADALWRAVRTLMFGAAFNLSNMENFEFTTLANDYEDGDYSGPVVNSRRQ
                     LWSVAGFVSLVVRTLAGFDAEMYGIAVTPSLPRAARNDFFVKGDRFILYDIPYRGKKV
                     TIDITLPPIGDANDGYYTAETVKLNGTLLNDPSAAEGDLDDENLFEIDISRHHAGVNT
                     ATVLPDTEPRTLFAPREPLVTGISSVGGHITLTLSRNGEPETDTAFTVYRNGEMVAEN
                     VTATSWSDADTLCLSAKTCCYSVSQRYLPAGHHSFPSNPQCFWGDSYERITTIAAAAL
                     TGSSGEVLATDHGKTHFSDWGTPTQTLTTTSPFAPTYTGQYYLQVEYGNGRPIDTGIT
                     ACQKFIDVYDTDGLVLVTSGPVVMPHLGANDWERWGNSSFLPVFLESGKSYSFVMRDG
                     MNMSYFAHFEPYGGAGGGTDGLNRVNLSALKVLRMTSE"
BASE COUNT          619 a         1025 c         1135 g          667 t
ORIGIN      
        1 aactcaagac gacggccgac gacaagacgc ttgccgccat cgccgatgaa tcgcagggcg
       61 acctgcggat agcgctctcc atcctcgaat cggccttcta cctttccgag tcgaaggaga
      121 tcgcgtggga aacggcacgc aaggtgctgc tgaaccccga caagtacgat aaaaaaggcg
      181 attaccacta ccgcatcata tcggccttca ttaagagtct gcgcggctca gaccccgacg
      241 cggcgctcta ttacctcgtc cggatgctcg acgcgggcga cgacccgctc ttcctcctgc
      301 gccggatgat cattttcgcc tcggaagaca tcgggaacgc cgatccgcgc gcgctggaac
      361 tcgccgtcgc ggcgcttcac gccttccgcc atatcggtct gccggaaggg gaactcaccc
      421 tcgcgcagtg cgtgacctac ctcgccaccg cgccgaaatc gaacgcatcc tacatggcca
      481 tcaaaaaagc gaaggacttc gtccgcgacc atcccgacct gaagccgccg caatacctcg
      541 tcaattcgac ctcgctcgca ccggaagagg aagagggcga ctacaaatat ccccacgact
      601 tccccggcca ctgggtcgat gtccgctatt tcccggaagg cgccgatccg caaagattct
      661 acgaccccac cgacatcggc tacgaaggga aactcaagac ctactgggac aaactcaaaa
      721 aggcggtccg ggaagggaag aagatgccgg tggagtgatc cttactcgct cgtcatccgc
      781 aacaccttca gcgctgagag attgacgcgg ttgagtccgt ccgtgccgcc gccggcgccg
      841 ccgtagggct cgaagtgggc gaagtaggac atattcatcc cgtcgcgcat cacgaaggag
      901 taactcttgc cgctttcgag aaagaccggc aggaacgacg agttccccca gcgctcccag
      961 tcgttcgcgc cgaggtgggg catcacgacc gggccgctcg tgacgaggac gaggccgtca
     1021 gtatcgtaca catcgatgaa cttctggcac gcggtgatgc cggtgtctat cggacggccg
     1081 ttgccgtact ccacctgcag atagtactgc ccggtgtagg tcggggcgaa cggggaggta
     1141 gtggtgagcg tctgtgtcgg cgtcccccag tcgctgaagt gggtcttccc gtgatcggtg
     1201 gccagcactt ctcccgacga cccggtgagg gcggcggcgg cgatcgtcgt tatccgctcg
     1261 taggagtcgc cccagaagca ttgcgggttc gaggggaacg aatggtgccc ggcggggaga
     1321 tagcgttgcg agacgctgta acagcaggtc ttggctgaaa gacacaaggt atcggcgtcg
     1381 ctccacgagg tcgcggtgac attctcggcg accatctcgc cgttgcggta gacggtgaag
     1441 gcggtgtcgg tctccggttc gccattgcgc gaaagggtga gcgttatatg gccgccgacc
     1501 gacgatatgc cggtgacgag cggttctctc ggcgcgaaaa gggtacgcgg ctcggtgtcg
     1561 ggcagtaccg tcgcggtgtt cacgccggca tggtggcggc tgatatctat ctcgaaaagg
     1621 ttctcgtcat cgagatcgcc ttcggccgcc gaagggtcgt tcaggagcgt cccgttgagt
     1681 ttcacggtct cggcggtgta atagccgtca ttcgcgtcac cgatgggggg aagcgtgatg
     1741 tcgatggtga cctttttacc gcgatagggg atatcgtaga ggatgaaccg gtcgcctttc
     1801 acgaaaaagt cgttacgggc cgcgcgcggc agggaggggg tcaccgctat gccgtacatc
     1861 tcggcgtcaa aaccggcgag cgtccgcacg acgagcgaca cgaacccggc gaccgaccac
     1921 agttgccgtc gtgagttgac cacgggaccc gaatagtcgc catcctcgta atcgttggca
     1981 agcgtcgtga actcgaagtt ctccatgttc gagagattga acgcggcgcc gaacatgagt
     2041 gtccggacag cgcgccaaag cgcatcggcg ttggcggtgt tccgcgccgc ctgtgcatag
     2101 agcgccgtca cgaagggcca gatggcgcgg ttgtggtaga tgggggtcca cggttgctgc
     2161 ggccagatca ccggcgctcc ggcgtcggtg tggggccaga acgctatctg cccgacggtg
     2221 gtccccgtat cgaagatcgt gtcgctccac tgcgcgagga gggccgtggc gagcaggtcg
     2281 tgacgcggaa gcggcgcatc gttgagttcg gtcagtttca ttgcgctcat gagcgtctgc
     2341 gtcgttccgg gcgcgaagcg agcggcgatg gcggcccgca ggtcgtcggt ccatgcggtg
     2401 tagcgtgtct gatcggcgct ttccccggcc atgaaggcga gttcggccgc tatccggagg
     2461 gcgcggtaat ggagttggtt ggtcgaaagc gacttcgaca tgccgatatg gaccgtgtcg
     2521 ttcgcggtcc atgcggggta ggtctgttcg cgccagtcga ggaacgaggt ctcgccgcgg
     2581 tacaggccgt cgtgcgggtc gtacgccacc gatcggtcgg tctcgacggt gttcttgagc
     2641 gccgcgtagg cgtcggtgag gaaagcggct cggtcggcgt cgctcaggta tttgagcgtt
     2701 tcgtaggcgc cgagcgccca gaccacgcgg tcggtcgata ccggccacga gccgccggag
     2761 ccggtgtcct gcactatctg cggcgcgccg ccgcccagcg tcgccttttt gtcggacagt
     2821 ttgaagagca gggagttcat cgtccgcacc ggatcgacga gggcgagccc gagataggcg
     2881 gcgtaggcgg tgtcgcgggt ccagacatag ggccatttct cgccggtgac aaagcagtcg
     2941 cacgccatcg gctgaccgcc gttgaacgag ccgtcggtta tctccgaaac gctgttctgc
     3001 cgggcctcct gtagcgcgag agcgaagagg gcgtcgaaca tgaggtggcc gctccgcagg
     3061 cgcatctgtc ccggttcctc gacgatggtc accttcttgt cgggcggata gttgtcgcgg
     3121 agcgtgttgt cggtcgaaag ttcataggta cggtaggtgt cgacggtggc ggtgacccac
     3181 gcggtgatgc cgttccacga tatcgaggcg gtggtgacta tctcgtcgat atcggtgtca
     3241 tcatcgggaa taggggcatc tgcatcgtcc aaaagatcgt ccggcaggtc ggacatgtcg
     3301 ggtagcggat cgtccccgtc ggtcagttcg tcgctttcgt cttttatgtc gctttcatcc
     3361 ttttcccccg tttcatcgtc gtccggctcc tctcctgcga gatcccagtc gggccacgaa
     3421 gcgttgtcct gaggcgcgct tttgcc
//
LOCUS       DAOQUE010000305         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31329_length_3444_cov_3.948067, whole genome shotgun sequence.
ACCESSION   DAOQUE010000305 DAOQUE010000000
VERSION     DAOQUE010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31329_length_3444_cov_3.948067"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PKH10_09270"
     CDS             <1..1162
                     /locus_tag="PKH10_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNT28353.1"
                     /translation="PRKYDAITGYRSRSMLVVPMKNHVGAVTGVLQLVNAVDRDRDAI
                     VPFDDKATDLVEAMASMAAVAIEKTKLLEDTRNLFNSVIEVLGVAMDAKSHYTGNHIQ
                     KVAQLNLELAQAIHEDDTVFPGVCFTENELEEIRLAGWLHDIGKVTTPEWVMDKDNKL
                     KTVVDRIALIEDRFRLAAEQLRTEGRTAEAEALLDDLRFVSDKNRPVEEMSDADIARL
                     REIASRRLLLAAHEERLINDDELRNLLIRHGSLTEEEIEKVKEHAVWTARMLDRLSFP
                     DHLRHVALYAVQHHEKLNGQGYPTGATADKIPLPSRILAIADLYEALSARDRPYKERM
                     PYEQTIAILRRYAENGDIDGRILSFFVEKDVFRRFEEKYRNGRNGASPLPRP"
     gene            1168..1869
                     /locus_tag="PKH10_09275"
     CDS             1168..1869
                     /locus_tag="PKH10_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT28354.1"
                     /translation="MHLSVIVPVYNEAATVGPVIEKLSSLPLPVGDSLEIVAVDDAST
                     DGSGITLAQLAGRGILRLITHPRNRGKGAALRSGIAAATGDRVLFHDADTEYDPADIP
                     RLLDAARSGLDIVYGSRFLDGGNAGATPLHALANRFLSLLTRLLTGLPTSDMETCYKL
                     VRADLLGRLDLREERFGIEPEITVKLGRLVRDEKLRYGEIPITYHPRGYDEGKKIGLR
                     DGLRAVWALFRYRFF"
     gene            1936..2376
                     /locus_tag="PKH10_09280"
     CDS             1936..2376
                     /locus_tag="PKH10_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT28355.1"
                     /translation="MPENKDTQFRSLKRYHTLKAELEHLVDSLCAPLASEPEEDIHFP
                     FDLFIENEELVVEVEMPGLEKEHLHIEGIDKFVEISGEKDTVKRKYKSCVGLERANGK
                     FKKLIYLEQAVNLQTATATLEDGILTIRLPLVEEKRGKKQITIT"
     gene            2385..>3444
                     /locus_tag="PKH10_09285"
     CDS             2385..>3444
                     /locus_tag="PKH10_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNT28356.1"
                     /translation="MEQQTPILPDQIKIPERLPVLALKDSVLFPFMIMPVFVSRDFSV
                     AGVNQAIASERLLFVTAQRRTDDEALSAETLYPVGCVASILKMQKLFDGRIKLLIQGV
                     QKARLKDLSRDATENYYAAAIEPIAEQTAAETDVRVEALVKNIRDGLDKLTNLGDKNF
                     PPDIVAILNSITDPIRFAELVVSHLSNSIEISQQVLEEGVAFKKLTIVNEFIEKELSL
                     LEIQNDIRNKTKERMGKAQKEYFLKEQMKQIQKELGTDDPYSYELEEYAQKVRDKNFP
                     DEVRTEAEKQITRLRSMNPAAPEVSVIKTFLDTLLELPWNDDRTEDNKDLNKAKEILE
                     EDHYGLEDIKERILEFLAV"
BASE COUNT          788 a         1113 c         1003 g          540 t
ORIGIN      
        1 accgcgcaag tacgacgcca tcaccggcta ccgctcccgt tccatgctcg tcgtgccgat
       61 gaagaaccat gtcggcgcgg tgaccggcgt cctgcaactg gtcaacgcgg tcgaccgcga
      121 ccgtgacgcg atcgtaccat tcgacgataa ggcgaccgat ctggtcgagg cgatggcatc
      181 gatggcagcg gtggcgatag agaagaccaa actcctcgag gacacccgca acctcttcaa
      241 ctccgtcatc gaggtgctcg gcgtcgcgat ggacgccaaa tcgcactaca ccggcaacca
      301 catccagaag gtcgcgcaac tcaatctcga gctggcgcag gccatacatg aggacgacac
      361 ggtgttcccc ggggtctgct tcaccgagaa cgaactggag gagatacggc tcgccggctg
      421 gctccacgac atcggcaagg tgaccacccc cgaatgggtg atggacaagg acaacaaact
      481 caagacggtc gtggaccgca tcgcgttgat agaggaccgg ttccgtctcg cggccgaaca
      541 gttgcgcacg gagggcagga cggccgaggc ggaggcgctg ctcgatgacc tgcgcttcgt
      601 cagcgacaag aatcgcccgg tcgaggagat gagcgacgcc gacatcgcac ggctccgcga
      661 gatagcgagc cgccgtctgc tccttgccgc tcacgaggag cgactcatca acgacgacga
      721 attgcgcaac ctcttaatcc gccacggatc gctcaccgag gaggagatag aaaaggtcaa
      781 ggaacatgcc gtctggaccg cgcggatgct cgaccggctc tcgttccccg accacctgag
      841 gcatgtggca ctctacgcgg tccagcacca tgagaaactg aacgggcagg gctacccgac
      901 cggcgccacg gccgacaaga taccgcttcc gtcacgcata ctcgccatcg ccgatctgta
      961 cgaggcgctc tcggcgcgcg accgccccta caaggagcgc atgccttacg aacagaccat
     1021 agcgatactg cgccgctacg ccgagaacgg cgacatagac ggccgcatac tttccttctt
     1081 tgtcgagaag gatgtcttcc gccgctttga ggaaaagtat cgcaacggcc gcaacggcgc
     1141 cagccccctc ccccgcccgt gaccgccatg catctgtcgg tcatcgtccc ggtctacaac
     1201 gaagcggcca ccgtcgggcc ggtcatagag aaactctcct cgcttcccct ccctgtgggt
     1261 gattcgttgg agatagtcgc ggtggacgac gcctcgaccg acggctcggg catcactctc
     1321 gcgcaactgg ccggacgggg catccttcgc ctcatcaccc atccccgcaa ccgcggcaag
     1381 ggggcggcac tccgctccgg catcgcggcg gcgaccggcg accgggtgct gttccacgac
     1441 gccgacaccg aatacgaccc ggccgacata ccacgactgc tcgacgcggc gcggtcgggg
     1501 ctcgacatcg tgtacgggtc gcgattcctc gacgggggca acgccggggc gacgccgctc
     1561 cacgcgctcg ccaaccgctt cctttcgctt ctcacccgcc tgctgaccgg cctgccgacc
     1621 agcgacatgg agacctgcta caaactggtg cgcgccgacc tgctgggacg gctcgacctg
     1681 cgcgaggagc ggttcggtat agaaccggag ataaccgtga aactggggcg tctggtccgc
     1741 gatgagaaac tccgttacgg cgagataccg ataacgtacc acccccgggg gtatgacgaa
     1801 gggaagaaaa tagggctccg cgacggcctg cgcgctgtct gggcgctgtt ccggtatagg
     1861 ttcttctgac ctccccttct cttgacttcg gcttttcctg attatatggt agaggaaccg
     1921 atccacgagg gaaccatgcc cgagaacaag gatacccagt tcagatcgct caagcggtac
     1981 cacaccctga aggcggagtt ggagcacctc gtcgattcgc tgtgcgctcc gctggcgagc
     2041 gagccggagg aggacatcca cttcccgttc gacctgttca tcgagaacga ggaactggtg
     2101 gtcgaggtcg agatgccggg gctcgaaaaa gagcacctcc acatcgaagg gatagacaag
     2161 ttcgtcgaga taagcggcga aaaagacacg gtaaaaagga aatacaagtc ctgcgtcgga
     2221 cttgaacggg ccaacgggaa attcaaaaaa ctcatctacc tcgaacaggc ggtcaacctc
     2281 cagaccgcga ccgcgacgct cgaggacggc atcctgacca tccgcctgcc gctcgtcgag
     2341 gagaagcggg gcaagaaaca gatcaccatc acctgaggaa cgatatggaa cagcaaacgc
     2401 ccatcctgcc cgaccagata aagataccgg agcgcctgcc ggtactcgcg ctcaaggact
     2461 cggtgctctt ccccttcatg atcatgccgg tcttcgtgag ccgcgacttc tcggtcgccg
     2521 gcgtcaatca ggccatcgcg agcgagcggc tgctgttcgt caccgcccag cggcgcaccg
     2581 atgacgaggc gctctcggcc gaaacgctct accccgtcgg gtgtgtagca tcgatactca
     2641 agatgcagaa actgttcgac ggccgcatca aacttctcat acagggggtg cagaaggcgc
     2701 ggctgaagga tctttcccgc gacgccaccg aaaactacta tgccgccgcc atcgagccga
     2761 tagccgaaca gaccgccgcc gagaccgatg tccgcgtcga ggcgctcgtc aagaacatcc
     2821 gcgacggact cgacaaactg accaacctcg gcgacaagaa cttcccgccc gacatcgtgg
     2881 cgatactcaa cagcatcacc gacccgatac ggttcgccga actggtggtc agccaccttt
     2941 ccaattccat cgagatatcc cagcaggtgc ttgaggaggg cgtcgcgttc aagaaactga
     3001 ccatcgtcaa cgagttcatc gagaaggaac tgtcgcttct cgagatacag aacgacatcc
     3061 gcaacaagac caaggagcgg atgggcaagg cgcaaaagga gtatttcctc aaggagcaga
     3121 tgaagcagat ccagaaggaa ctcggcaccg atgacccgta cagttacgaa ctggaggaat
     3181 acgcccagaa ggtccgcgac aagaacttcc ccgacgaggt gcgcaccgag gccgaaaaac
     3241 agataacccg cctgcggagc atgaacccgg ccgcccccga ggtgagcgtg ataaagacct
     3301 tcctcgatac gctcctcgaa ctgccgtgga acgacgaccg caccgaggac aacaaggacc
     3361 tcaacaaggc gaaggagata cttgaggagg accactacgg gctcgaggac atcaaggagc
     3421 gcatcctcga attcctcgcc gtgc
//
LOCUS       DAOQUE010000306         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31333_length_3444_cov_3.439658, whole genome shotgun sequence.
ACCESSION   DAOQUE010000306 DAOQUE010000000
VERSION     DAOQUE010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31333_length_3444_cov_3.439658"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PKH10_09290"
     CDS             <1..621
                     /locus_tag="PKH10_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28357.1"
                     /translation="PTDDEDWESLTTPPSRPERLAIMKRRSLVVFAILFLVLMALTAY
                     IFYEPPAPDVTGPRTRDTLAPACRGGNGVACAELAFLEYRGLSEVTGNIGEKARNPAN
                     AFIYGRLACAKRSAFGCYLMWRLWTKKETELLKPQSAEETLRNGCKAGNRMLCAFEKR
                     FAGARDIAPQDLQATIVESAGGKNPPRYAKDNREQLLFELFLRDDQ"
     gene            636..1037
                     /locus_tag="PKH10_09295"
     CDS             636..1037
                     /locus_tag="PKH10_09295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNT28358.1"
                     /translation="MERIFVVGKGDCDRFGLVRYRRYLDWVHETRAAFMQELGAGDDL
                     LASKGIDMVTVNLDLHNLEACFERDEVIVTPTLDKAMDHAVHFTYTGKARRTGKTIFI
                     AHIEMYPISAEGRLMRFPPEIADHLLNQIGA"
     gene            complement(1048..2601)
                     /locus_tag="PKH10_09300"
     CDS             complement(1048..2601)
                     /locus_tag="PKH10_09300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT28359.1"
                     /translation="MGAMDIVRYLYFIPPVIVLFLAFYAWPKRRVPTVQPFIGLMMGL
                     FFYTFAFAMGHFVSTVATLRLWDRVMYVGLATVPSFALVLALVFSNREYWSHRLVRTV
                     LVAIPFLTLVLNYSTELHGLYYASLGLAPEGSASRLLFSPGPWYHVHMGYINMAAVVG
                     FLFYAEMLLNAAATRYRNRSLLMMGGVLLPWGAYLISQSGWAPTGLDIVPYASALAGL
                     LYAAGLFGFGTLDLIPIARERVFDAIAEGVIVFDTRHRLLDFNAAARSILPALDKAPI
                     GASMSEVLTVFPTLATQIIDGVPGVDIELQSGGRTRYFYAYYAPITNRFGTRIGGTLM
                     LADITDRELLMRSLEQISVVDALTGLSNRRSLMVHIDREISRAKRHKRPLSLIMLDID
                     HFKQVNDRWGHLAGDEVLRAVSDACRDAVREHDLVGRYGGEEIVVVLPEAQIDDAMVI
                     AERIRQKIAEVAVRYGDDRISITASSGAASLDMLPEEKRTTDRFIEVTDRAMYRAKAE
                     GRNCVRRAG"
     gene            complement(2620..3363)
                     /locus_tag="PKH10_09305"
     CDS             complement(2620..3363)
                     /locus_tag="PKH10_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT28360.1"
                     /translation="MSLLTVEQLTVGYGETVILQDVSFSVGAGERFVVLGTSGCGKST
                     LLKAIVGLNPPVKGRIFFSGEEIVHPLPEETPFYRRIGILYQSAALLNSMTLEENVML
                     PLKMHFPRLADAAARDMAGEKLSMVNLYEHRRKLPSELSGGMKKRGALALAMVLAPEI
                     IFFDEPQAGLDPVTTRELDLLFIRLNERMGITFFTVTHELLSIRRSAQKVLMLADGGV
                     AFLGSLDEAMMTDEFRVRRFFDVGSEKSM"
BASE COUNT          699 a         1112 c         1032 g          601 t
ORIGIN      
        1 cccacggacg acgaggactg ggagtccctc accacgccac cctcccgccc cgaacgactg
       61 gccatcatga agcgtcgatc tcttgtcgta tttgcgatcc tcttcctcgt tctgatggct
      121 ctgaccgcct acatcttcta cgaaccgccc gcgcccgatg tgaccggccc gcgcacccgt
      181 gacaccctcg cccccgcctg ccgcggcggc aacggcgtcg cctgcgccga gctcgcgttc
      241 ctcgaatacc gggggctttc ggaggtgacc gggaacatcg gggaaaaagc acggaacccc
      301 gccaacgcct tcatctacgg tcgcctcgcc tgcgccaaac gatcggcgtt cggctgctat
      361 ctcatgtggc ggctctggac taaaaaagag accgaactgc tgaaaccgca gagcgccgag
      421 gagacgcttc ggaacggctg caaggcgggc aacaggatgc tctgcgcctt tgaaaaaagg
      481 ttcgccgggg cacgggacat cgccccgcag gacctgcagg cgaccatcgt ggaatcggcg
      541 ggaggcaaga atccgccgcg ttacgccaag gacaatcgcg aacagttgct cttcgaactg
      601 ttcctgcggg acgatcaata acaggagggt ttcccatgga aaggatcttc gtggtcggaa
      661 agggcgactg cgaccgtttc ggactggtcc gctatcgtcg ctatctcgac tgggtacacg
      721 agacgcgcgc cgctttcatg caggaactag gcgcggggga cgacctgctt gccagcaagg
      781 ggatcgacat ggtgacggtc aatcttgacc tgcataacct cgaggcctgt ttcgaacgcg
      841 acgaggtcat cgtgaccccg acactcgata aggccatgga ccatgcggtc cacttcacct
      901 acaccgggaa ggcccggaga acggggaaaa cgatattcat cgcgcatatc gagatgtacc
      961 cgatatcggc ggaaggtaga ctgatgcgct tccctcccga gatcgccgat cacctcctca
     1021 atcagatcgg cgcctgaacc ccgccactca ccccgcccgg cgcacgcagt tgcgcccttc
     1081 ggccttcgcg cggtacatcg cccggtcggt gacctcgatg aagcgatcgg tcgtccgttt
     1141 ctcttcgggc aacatgtcca gcgatgcggc tccggaactc gccgtgatcg atatgcggtc
     1201 gtcaccgtaa cggaccgcga cctcggctat cttctgacgg atacgctcgg cgatgaccat
     1261 cgcgtcatct atttgcgcct ccggaagcac caccacgatc tcctcgccgc cgtaccttcc
     1321 gaccagatcg tgctcgcgca ccgcgtcgcg gcacgcgtcg ctcacggcgc gtagcacttc
     1381 gtcgcccgcc aggtggcccc agcggtcgtt cacctgcttg aagtgatcga tatccagcat
     1441 gatgagggac agcggtcgtt tgtgccgttt cgcacgactg atctcccggt ctatatggac
     1501 catgagcgag cgacggttgc tgagcccggt gagggcgtcg actaccgata tctgctccag
     1561 actgcgcatc agcagttccc tgtcggtgat gtcggcgagc atcagcgtgc cgccgatccg
     1621 cgtaccgaag cggttggtta tcggcgcata gtaggcgtag aaatagcggg tccgcccacc
     1681 gctctgcagt tcaatgtcga cccccggcac cccgtcgatg atctgcgtcg cgagcgtcgg
     1741 gaacaccgtg agcacctccg acatggaggc gccgatcggc gccttgtcga gcgccggcag
     1801 tatgctgcgc gccgccgcgt tgaaatcgag caatcggtga cgcgtgtcga atacgatgac
     1861 cccctccgcg atcgcgtcga acacccgttc ccgcgctatc gggatgaggt cgagcgtgcc
     1921 gaaaccgaac agtccggcgg catacagaag ccccgccagg gccgaggcgt agggcacgat
     1981 gtccagcccg gtcggcgccc atccgctctg cgagatgaga taggcgcccc acggcaggag
     2041 cacgccgccc atcatcagca gggaacggtt gcggtaacgg gtcgcggcgg cgttcaggag
     2101 catctcggcg tagaacagga acccgacgac cgccgccata ttgatgtacc ccatgtggac
     2161 atgataccac gggcccggag agaaaaggag acgggatgcg ctcccttccg gcgccagacc
     2221 cagagaagcg taataaaggc cgtggagttc ggtgctgtag ttgagcacga gcgtcaggaa
     2281 cgggatcgcg acgagcaccg tacggaccag ccggtgcgac cagtactcgc gattgctgaa
     2341 tacgagcgcg aggacgagtg cgaacgacgg gacggtggcg agtccgacgt acatcacgcg
     2401 gtcccagagc cgcagcgtag cgacggtgga aacgaaatga cccatcgcga aggcgaaggt
     2461 gtagaagaac aggcccatca tcagcccgat gaagggttgc accgtcggca ctctccgttt
     2521 cggccacgcg tagaaggcaa ggaacagcac gatgaccggc gggatgaaat acagatagcg
     2581 gacgatatcc atggcaccca tgacccctcc cctcacccgt tacatactct tttccgatcc
     2641 cacatcaaag aaccgccgga cccggaactc gtcggtcatc atcgcctcat cgagcgatcc
     2701 gaggaacgcc accccgccat ccgcgagcat cagcaccttc tgtgcgctac gccgtatcga
     2761 aagcagttcg tgcgtcacag tgaagaaggt gatgcccatc cgctcgttga gtcgtatgaa
     2821 gagcaggtcg agttcgcggg tcgtcaccgg gtcgagcccc gcctgcggct cgtcgaagaa
     2881 gatgatctcg ggggcgagca ccatcgccag ggcgagtgct ccccgtttct tcatgccgcc
     2941 cgaaagttcc gacggcagtt tccggcggtg ctcgtacaga ttcaccatgg agagtttctc
     3001 ccccgccatg tcgcgggccg ccgcgtcggc aagccgcggg aaatgcatct tgagcggcag
     3061 catcacattc tcttcgagcg tcatggaatt gaggagcgcg gcgctctgat acaggatgcc
     3121 gatgcgacga tagaacgggg tctcctcggg aagcgggtgg acgatctcct caccggagaa
     3181 gaagatgcgc cccttcaccg gcggattgag cccgacgatc gccttgagga gtgtcgattt
     3241 accgcacccg gaggtgccga gcacgacgaa tcgctccccc gccccgaccg agaaggagac
     3301 atcctgcagg atgaccgtct cgccgtatcc gaccgtcaac tgttcgaccg taagcagtga
     3361 catcagcgcc acctcccgcc gctgttgtac ccatttccga tattttttcc aataattttg
     3421 gcgtgaagca cgccaaataa cttg
//
LOCUS       DAOQUE010000307         3430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31499_length_3430_cov_4.203259, whole genome shotgun sequence.
ACCESSION   DAOQUE010000307 DAOQUE010000000
VERSION     DAOQUE010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31499_length_3430_cov_4.203259"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1564)
                     /locus_tag="PKH10_09310"
     CDS             complement(134..1564)
                     /locus_tag="PKH10_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT28361.1"
                     /translation="MRHLPLFLTIVLLAAIVSCATGEKIAEEIQSEKQRLSVDEKRGA
                     RVCVPYDLAMARVALASAQMELEAGNYHIAEDLLLKEKKYAANTAKYADSCIPKDKDE
                     DGVPDDADKCPLQPGPAKYDGCPDSDGDGVLDLDDKCPSVPGLRERQGCPDAADSDSD
                     GIPDNKDRCPFDAEDVDGFEDKDGCPDTDNDKDGVLDDIDKCRDQAGPASNQGCPFHD
                     KDGDGIPDEMDKCPTDPEDGDGFEDQDGCPESDNDKDGLCDENPVIQKSIDKFKSACV
                     GADKCPNDPGPRENHGCPVLDGDGDGVPDVIDKCPAEAGPMTNQGCPVSDRDGDGIVD
                     KDDKCPDEPGPIEEQGCPKKYKLIVVTAQKIELKQTVFFKTGKAVIDGKSYPMLKEVA
                     DAIKSVPSIKKVLIEGHTDNVGGRNLNIKLSQSRADSVREFLIDEGVDGSKLEATGYG
                     PDKPISPNKTAKGRAMNRRVEFNIVQ"
     gene            complement(1561..2316)
                     /locus_tag="PKH10_09315"
     CDS             complement(1561..2316)
                     /locus_tag="PKH10_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28362.1"
                     /translation="MNDIVIKMSLFLLAVLSFLSCGTIQSVSAVKEADAARDIALDEL
                     ARTYQDTRLILTDRYGHRSLSPEAATFVEDRDRRDPIYLFYMTDGCIVAARRLQAKSE
                     HERARDMAVKGKEYAEKTVAALKVLSPVAPQESPAAPDPIMAPYAETPAPAPAPAPAP
                     QVEKPQPAPQPKPAPAPAPRVEKPQPAPKPAPAPEPVVPTTVKEEKKEKEEKKEDEKK
                     EEKKGKKEENYYELYERLKREAEEKNKQEGGAK"
     gene            complement(2389..>3430)
                     /locus_tag="PKH10_09320"
     CDS             complement(2389..>3430)
                     /locus_tag="PKH10_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28363.1"
                     /translation="IDDDACRFLSRAVVHILKRGFKIEPDDRKFYRDIVGRLSAVSDI
                     PDANRHWLLLSYLNLGGITERKYNSFLYSSAIYMEKLGFFELAQRCYQSILSSFGKDQ
                     EDKRFALYLELKNAKLWGFSDPSWARLFWQTMLNTAKNEGLLHIQLLSQGELLLLETE
                     KIVLHEIVEIVKQLHKAGCYEDEISLIDRMSDLLLKTNNFSDAKIFASRAFRIMEDIR
                     SRNKDDNTAGGDIDPATIIFMRASCKLADVHAALREYPDALQLLQSAMDLAVATNISY
                     FKAKILLSLGKIRMVLGEEWEPLVQDGFTQALQGMDFAIMKSYFLFFEEQGLEARPWV
                     QPHIEYKNWINF"
BASE COUNT          569 a          985 c         1050 g          826 t
ORIGIN      
        1 cgcgacggtc acctcgatgt gaacggtcag ccgggggcct gagcgctgat tggagaatac
       61 cgcgccgtcc ttctgttttt cgtcgtattc cggcgtcatc atccttctcc gttcagtgta
      121 gaggaaggaa aggtcattgg acgatattga actcgacgcg gcggttcatc gcccgtccct
      181 tcgcggtctt gttgggcgat atcggcttat cggggccgta tcctgtcgcc tcgagtttcg
      241 agccgtcgac cccctcatcg atgaggaact cgcggaccga atcggcgcgg gactgggaga
      301 gtttgatgtt caggttgcga ccgcccacat tgtcggtgtg tccctctatc agcaccttct
      361 tgatgctcgg tacgcttttt atcgcgtcag cgacctcctt gagcatgggg taggatttac
      421 cgtctatcac cgccttgccg gtcttgaaga acaccgtctg cttgagttct atcttctgcg
      481 cggtcaccac gatgagtttg tatttcttcg ggcagccctg ttcctcgatc ggccccggtt
      541 cgtcgggaca cttatcgtcc ttgtcgacga tcccgtcgcc atcgcgatca ctgaccgggc
      601 acccctggtt ggtcatcggg ccggcctcgg cggggcattt gtctatcaca tcgggaacgc
      661 cgtcaccgtc gccgtcaagc accggacagc cgtggttctc gcgtggtccc ggatcattcg
      721 gacacttgtc ggcgccgaca caggcgctct tgaacttatc gatgcttttc tggatgaccg
      781 ggttctcatc gcacaacccg tccttgtcgt tgtcggattc gggacagccg tcctgatcct
      841 caaaaccgtc accgtcctca ggatcggtgg ggcatttatc catctcgtcg ggtatgccgt
      901 cgccgtcctt gtcgtggaag ggacagccct gattggacgc gggaccggcc tggtcgcggc
      961 acttgtctat atcgtcgagc acgccgtcct tgtcgttatc ggtgtccgga cacccgtcct
     1021 tgtcctcgaa accgtctaca tcctcggcat cgaaggggca gcggtccttg ttgtcgggta
     1081 tgccgtcgct gtcgctgtcg gcggcatcgg gacagccctg tcgttcgcga aggcctggga
     1141 cgctggggca tttgtcgtca agatcgagta cgccatctcc gtctgaatcg gggcatccgt
     1201 cgtatttcgc cgggccgggc tggagcgggc acttatcggc atcatcgggc acaccgtcct
     1261 catccttgtc cttgggtatg caggagtcgg cgtatttcgc ggtgttcgcg gcgtatttct
     1321 tctccttgag caggagatct tccgcgatgt ggtaattgcc cgcctcaagt tccatctggg
     1381 cggaggcgag cgccacgcgg gccatggcga ggtcgtaggg gacacagacg cgggcgcccc
     1441 gcttctcatc gaccgacagc cgttgtttct cggactgtat ctcctccgct atcttttcgc
     1501 cggtggcgca ggaaacgatg gccgccagca ggacgatcgt caggaacagg ggaaggtgtc
     1561 tcatttcgcg cctccctcct gcttgttctt ttcttccgct tcacgtttca gccgctcgta
     1621 gagttcgtag tagttctctt ctttctttcc cttcttttct tccttcttct cgtcctcttt
     1681 cttctcctcc ttttcctttt tctcttcctt caccgtcgtc gggaccaccg gttccggcgc
     1741 gggagctggc ttcggtgccg gttgcggttt ttccacccgg ggagcgggag ccggtgccgg
     1801 cttaggttgt ggtgccggtt gcggtttttc cacctgagga gcgggagccg gtgccggagc
     1861 gggagccggc gtttcggcgt acggggccat gatcgggtcg ggcgcggcgg gactttcctg
     1921 cggagcgacg ggagagagca ccttcagggc cgcgacggtc ttttcggcgt actctttgcc
     1981 tttgaccgcc atgtcgcgcg cccgctcgtg ctcgcttttc gcctgcagtc tgcgcgcggc
     2041 cactatgcaa ccgtcggtca tgtagaaaag atatatcgga tcgcggcgat cgcgatcctc
     2101 gacgaaggtc gccgcctcgg ggctcagcga acggtggccg tatcggtcgg tgaggatgag
     2161 ccgggtatcc tgataggtgc gggccagttc atcgagcgcg atgtcgcgtg ccgcgtcggc
     2221 ctctttgacg gccgacaccg actggatggt gccgcaggac aaaaaggaga gaacagccaa
     2281 caaaaacaaa gacatcttga taacgatatc gttcatggcg ccctcacaag gaacctcagg
     2341 gaacgaccct atcagaaaac gaggggtgag tcaagtttcc cacccccgct aaaaattgat
     2401 ccagttcttg tattcgatgt gcggttggac ccacggtcgc gcctccaatc cctgctcctc
     2461 gaagaagagg aagtaggact tcatgatggc gaaatccata ccctgcaggg cctgcgtgaa
     2521 cccgtcctgt acaagcggct cccattcctc gcccagcacc atccgtatct tgccgagcga
     2581 gagcagtatc ttcgctttga agtaggatat gttggtcgcc acggcgaggt ccatggcgct
     2641 ctggaggagt tggagcgcgt cggggtattc acggagcgcc gcgtgcacat cggcgagttt
     2701 gcaggaggcc cgcatgaata tgatggtcgc cggatctatg tctccgccgg cggtgttgtc
     2761 gtccttgttg cgtgaacgga tgtcctccat gatgcggaaa gcgcgcgagg cgaatatctt
     2821 ggcgtcggag aagttattcg tcttgaggag caggtcgctc atccggtcga tgagcgatat
     2881 ctcgtcctcg tagcatcccg ccttatgcag ttgtttgacg atctcgacga tctcgtggag
     2941 gacgatcttt tcggtctcga ggagcagcag ttcgccctga ctgagaagtt ggatatgcag
     3001 gagcccctcg ttcttggcgg tgttaagcat cgtctgccag aacaggcgag cccacgaggg
     3061 gtcggaaaat ccccagagtt tcgcgttctt gagttcgaga tagagcgcga aacgtttgtc
     3121 ttcctggtcc ttgccgaacg acgaaaggat ggactggtaa cagcgttgcg cgagttcgaa
     3181 gaatccgagt ttctccatgt agatggcgga ggagtagagg aaggagttgt atttccgttc
     3241 ggtgatgccg ccgaggttga ggtagctcag gagcaaccaa tggcggttgg cgtcgggaat
     3301 gtcgctgaca gccgacaggc gaccgacgat gtcgcggtag aatttgcggt cgtccggctc
     3361 tatcttgaaa ccccgtttga ggatgtggac caccgcgcgc gagaggaagc gacaggcatc
     3421 gtcatcgatg
//
LOCUS       DAOQUE010000308         3422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31614_length_3422_cov_3.324918, whole genome shotgun sequence.
ACCESSION   DAOQUE010000308 DAOQUE010000000
VERSION     DAOQUE010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31614_length_3422_cov_3.324918"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PKH10_09325"
     CDS             complement(<1..235)
                     /locus_tag="PKH10_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT28364.1"
                     /translation="MPTVSFTVDLDPTPAYYAIHGLPWSGGEDPILKSGLRRLIDLLK
                     EEGIPATLFVTGGSLGPDEWALLREAADAGCELA"
     gene            complement(240..959)
                     /locus_tag="PKH10_09330"
     CDS             complement(240..959)
                     /locus_tag="PKH10_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT28365.1"
                     /translation="MEKKLVTIVIPVYNEEQILEQAVTGLYDELEKYRADFDFELILA
                     ENGSKDNTIAKGEELAKRFPGMSIFHHPEPDYGNALKAGIRRAKGEIIVCDEIDLCLP
                     EFYEKALAVFKANGAQMIVGSKAMKGAKDERPFKRKFATFVLNLLLKIILGFKGTDTH
                     GVKAFLKAPLMPVLDKCLVGKDIFASEFVIRAERAGIDIREIPISLDEKRAPSIRLLK
                     RVPRVISNLAYLFWAIRIRRI"
     gene            complement(973..3030)
                     /locus_tag="PKH10_09335"
     CDS             complement(973..3030)
                     /locus_tag="PKH10_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT28366.1"
                     /translation="MPKRRELSFLKKRKQMRDRLRALSSTPQAIAISEYRQRIIQAIR
                     MAGGTEASSDHLISILDDILRSKSEITVEEIDALFPENADFSVDDMELLFNVLDEFKI
                     PVISEKIDEESFPTDLPIIRKPHIPQDRGRKDPLRMYMLTIGSVPLLTKEGEVAIAQR
                     IEEGEKEIIRTLLSVFISFKEIISIPDRILNEQLSLKEVVREADEELADEEDEREEVV
                     EAAEDDEDEELKELSEGKIGEIPDEDDEEDDEEDDEDEETEETPAPAATAPAKEPEPE
                     KDPALERQRQIIETLSRHIDRLKQLNRERERMLKQTLSARTRKMKDRLADDRLDQMVA
                     VVLDMRLNKNYLKTLGDRIRMYRDKVEQLDRSIAQIERSLNMGFAQMNEIISRPQRFS
                     VMDKRNMQQIREKYTLIVQTKRSVKKLEAETGLSAKDLKACADQLYRAEESVRLAKNE
                     LIQANLRLVVSIAKKYNNRGLDFKDIIAEGNIGLIKAVDKFEYRRGYKFSTYATWWIR
                     QSITRAIADQGRTIRIPVHMIEMMNKINKTIKELTNKNGYEPGIADVAKAMNIPEEKI
                     RKVWRSARETISLETPIGEDEDSQLQDFVEDPKTMGPEEYAAKQNMAEIIRDLFDGLT
                     PREEKVIRMRFGVGETETYTLEEIGKDMGVTRERIRQIEAKAVMKLRKMLKQKRTSWE
                     DFS"
     gene            complement(3023..>3422)
                     /locus_tag="PKH10_09340"
     CDS             complement(3023..>3422)
                     /locus_tag="PKH10_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28367.1"
                     /translation="LAATHPTLIPALDDPVLDRYDRADVRRALLDAYDAGEGDAVYRR
                     IIGKVTEDEHAPGAPTPLDADAAEMRFRSLLSQIHIAAIDETLRDLAGASNPTPEEIA
                     EIQRLRTEKKQWSDALIGVRSSTDVRRTHA"
BASE COUNT          517 a          979 c         1068 g          858 t
ORIGIN      
        1 cggccagttc gcagccggcg tcggccgcct cgcgcaagag cgcccattca tcggggccaa
       61 gcgatccgcc ggtgacgaac aatgtcgccg ggatgccttc ttctttcagc agatcgatga
      121 gccgtcggag cccgcttttc agtatcggat cctcgccgcc gctccatggc agaccgtgga
      181 tggcatagta ggcgggggtg gggtcgagat cgaccgtgaa agatacggta ggcatcgtct
      241 tatattcttc tgatgcgtat ggcccagaaa aggtaggcga ggttggagat gacgcggggg
      301 acccgtttga ggagccgtat cgaaggagcc cgtttttcgt cgagcgatat cggtatctcg
      361 cggatgtcga tgccggcccg ctcggcgcgg atgacgaatt cggaggcgaa gatgtccttg
      421 ccgacgaggc atttgtcgag caccggcatg agcggcgctt tgaggaacgc cttgacgccg
      481 tgggtgtcgg tccccttgaa gccgaggatg atcttgagga gcaggttcag cacgaaggtg
      541 gcgaatttgc gcttgaaggg gcgttcgtcc ttggccccct tcatcgcctt ggaaccgacg
      601 atcatctgcg cgccgttcgc cttgaagacg gcgagcgcct tctcgtagaa ctccggaagg
      661 cagaggtcta tctcgtcgca gacgatgatc tcccccttgg cgcggcggat gcccgccttg
      721 agggcgttgc cgtagtcggg ttcggggtgg tggaatatcg acatgccggg gaaccgtttg
      781 gcgagttcct cgcctttggc gatggtgttg tccttggagc cgttctcggc cagtatcagt
      841 tcaaagtcga agtcggcgcg gtacttctcc agttcgtcgt agaggccggt gaccgcctgt
      901 tcgagtatct gttcctcgtt gtagaccgga atgactatcg tgaccagttt cttttccatc
      961 gctcggtccc tcttacgaga aatcttccca actggtccgt ttctgtttga gcatcttgcg
     1021 gagtttcatg accgccttcg cctcgatctg gcggatgcgt tcgcgggtga cgcccatatc
     1081 cttgccgatc tcctcgaggg tgtaggtctc ggtctcgccg acgccgaagc gcatgcggat
     1141 gaccttctcc tcgcgcgggg tgagcccgtc gaacaggtcg cggatgatct cggccatgtt
     1201 ctgcttcgcg gcgtactcct cggggcccat cgtcttcggg tcctcgacga aatcctgaag
     1261 ctggctgtcc tcatcctcgc cgatgggcgt ttcgagcgat atcgtctcgc gcgccgagcg
     1321 ccacaccttg cggatctttt cttccggtat gttcatcgcc ttggcgacat cggcgatgcc
     1381 cggctcgtag ccgttcttgt tggtcagttc cttgatggtc ttgtttatct tgttcatcat
     1441 ctcgatcatg tggaccggta tgcggatggt ccggccctga tcggcgatgg cgcgcgttat
     1501 cgactggcgt atccaccagg tcgcgtaggt cgagaacttg tagccgcggc ggtattcgaa
     1561 cttgtcgacc gccttgatga gcccgatgtt gccctcggcg atgatgtcct tgaagtcgag
     1621 ccctcggttg ttgtacttct tcgcgatgga gacgaccagc cgcaggttgg cctgtatgag
     1681 ctcgttcttc gcgagtcgga ccgattcttc tgcgcggtag agttggtcgg cgcaggcctt
     1741 gagatccttt gccgacaatc ccgtttcggc ctcgagtttc ttgacactgc gcttggtctg
     1801 gacgatgagc gtgtatttct cgcgtatctg ctgcatattg cgcttgtcca tgacggagaa
     1861 gcgctgaggc cgcgagatga tctcgttcat ctgggcgaaa cccatgttga gcgagcgttc
     1921 gatctgggcg atgctccggt cgagttgctc gaccttgtcg cgatacatgc ggatgcggtc
     1981 gccgagcgtt ttgagatagt tcttattgag ccgcatgtcg agtacgacgg cgaccatctg
     2041 gtcgagccgg tcgtcggcga gccggtcctt catcttacgc gtccgggccg acagcgtctg
     2101 cttgagcatc cgttcgcgtt cgcggttgag ttgtttgagc cggtcgatgt gacgggagag
     2161 tgtctcgatg atctggcgct gacgctcgag cgcggggtct ttttccggct cgggctcttt
     2221 cgcgggggcc gtcgcggcgg gggcaggcgt ttcctccgtc tcctcgtcct cgtcgtcctc
     2281 ctcgtcgtcc tcctcgtcgt cctcgtcggg tatctcgccg atcttccctt cacttagttc
     2341 cttcagttcc tcgtcctcgt catcctcggc cgcttcgacg acctcttccc gttcatcttc
     2401 ctcatcggcc agttcctcgt ccgcctcgcg gacgacctcc ttgagagaaa gttgctcgtt
     2461 gagtatccgg tcgggtatgg agatgatctc cttgaacgag atgaagacgg aaagcagggt
     2521 acgtatgatc tccttttcgc cttcctctat gcgttgtgct atggcgacct cgccctcctt
     2581 ggtgagcaag ggcaccgaac cgatggtgag catgtacatg cggagcggat ccttgcgacc
     2641 gcggtcctgc gggatgtgcg gtttccggat tatcggcagg tcggtgggga acgattcttc
     2701 gtctatcttc tccgagatga ccggtatctt gaattcgtcg agcacattga atagcaattc
     2761 catgtcatcc accgagaaat cggcgttctc ggggaatagg gcgtctatct cctcgacggt
     2821 gatctcgctc ttgctgcgca ggatgtcgtc aagaatgctg atgagatggt cgctgctcgc
     2881 ctcggtgccg ccggccatcc tgatggcctg gatgatccgc tggcgatatt cgctgatcgc
     2941 gatggcctga ggggtcgacg aaagcgcacg caagcgatcc cgcatctgct ttcttttttt
     3001 caggaatgag agttctcttc ttttaggcat gcgttctcct cacatcggtg cttgatctga
     3061 cccctatgag agcatcggac cactgtttct tttcggtgcg aaggcgctgt atctccgcta
     3121 tttcctcggg ggtgggattg gatgcgcccg ccagatcgcg gagcgtctcg tctatggcgg
     3181 cgatatgtat ctgtgacagc agggaccgga acctcatctc ggcggcgtcc gcgtcgagcg
     3241 gcgtcggagc gcccggtgcg tgttcgtcct cggtgacctt tccgatgatg cggcggtaga
     3301 ccgcgtcccc ttcgcccgcg tcgtaggcat ccaggagggc gcgacgaaca tcggcgcgat
     3361 cgtaccggtc gagtaccgga tcgtcgagcg ccggtatcag cgtcggatgg gtcgcggcga
     3421 gc
//
LOCUS       DAOQUE010000309         3416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31692_length_3416_cov_3.825647, whole genome shotgun sequence.
ACCESSION   DAOQUE010000309 DAOQUE010000000
VERSION     DAOQUE010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31692_length_3416_cov_3.825647"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PKH10_09345"
     CDS             complement(<1..899)
                     /locus_tag="PKH10_09345"
                     /inference="COORDINATES: protein motif:HMM:NF027515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNT28368.1"
                     /translation="MQTVEKIALLRARMAEQGLNALIVPSTDPHQSEYVAAHWQTRAW
                     LSGFNGSAGTLVVTKDKAGLWTDSRYFLQAEDQLAGSGITLFKMGLPETPAHTAWLAS
                     ELAAGAVVGIDPSLFSINAFRALEKAMAAKGIALRSVGDLAGSLWTDRPPLPADRAIL
                     HDDDIAGEGRTSKLSRLRAKMTEKNADWHIISTRDDIAWLYNIRGSDVTCNPVVIAYA
                     AVSRDEAFLFTDTAKFDAKARKALAADGVTLKKYDYLERLLKKIRKQRILIDPDRNGR
                     HILDTLGKKAGIVEGPAIPLLLKA"
     gene            complement(985..3318)
                     /locus_tag="PKH10_09350"
     CDS             complement(985..3318)
                     /locus_tag="PKH10_09350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNT28369.1"
                     /translation="MRSSRSISSDIPAAAITAYARERNLTPREIAFILKGETRLVRAD
                     LREALPPWTALLREEPILSLFPLVSRLLAGDPAHFLAAGEKLVSFLAQDIESVVPLST
                     GQLRKMKKELGDTVFHLLVETLPERYETRILTADLAGVAPGDIATATGTVMKWKPAWR
                     ATAPWTMEMAIGNAKIPVSFFGAIGKGYAAQFPEGSEIIVSGEVSRRTVIPSFTNPDI
                     FRYDEVWKELLSGVMPIYRRIPGVSRLFHLRAVREASLRLMKFDGDWLPPVARGRYDW
                     PDLILSILNVHFPASDLPAAETAALDTPYHRRLAFDKMFFFQYGSHLLRRETAPRKPR
                     TVHPFSKRAADIEKALPFSLTNAQRRVLAEIRRDLAAPEPMMRLLQGDVGSGKTIVML
                     LAALDVIASGYRAVIMAPTEILARQHHDTITRCVPGVEAALAVGGRSGKKKKEMLDSF
                     RDADLIVGTHALYENLEALPDLGLIVIDEQHRFGVSQRMELMGKATAPDVLVASATPI
                     PRSLALTIYGGVSISVLNELPPGRIPVKTRSVPFANRHKVIEHVVSIIREDGKKGYWI
                     CPLVEGSDKSDLAPVTAVYEEFRALIGDRARLLHGRMKGEEKDAALAALRDGSANLLV
                     STVVVEVGVDVPDATFIAVENAERFGLAQLHQLRGRVGRGAERSFAAFIAGEQVSEKA
                     LERLAFMERTSDGFKIAEFDLRLRGPGALSGLEQSGFRNDPYYLLAARYGIEVQKATQ
                     AARSLLSSGDIAPAEREFVSRVFATFFRERFEKIRVG"
     gene            complement(3290..>3416)
                     /locus_tag="PKH10_09355"
     CDS             complement(3290..>3416)
                     /locus_tag="PKH10_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNT28370.1"
                     /translation="GKISVRSPIGGALIGREVGDEVTVQTPSGARKYEIVSVNFI"
BASE COUNT          582 a         1038 c         1162 g          634 t
ORIGIN      
        1 gccttgagca ggagcggtat cgccgggcct tccacgatgc cggccttctt gccgagcgtg
       61 tcgagtatgt ggcgaccgtt gcggtcaggg tcgataagga tgcgctgttt gcgtatcttt
      121 ttgagaagcc gttcgaggta gtcgtacttt ttcagcgtga cgccgtcggc ggcgagcgcc
      181 ttgcgggcct tcgcgtcgaa cttcgcggtg tcggtgaaca ggaacgcctc gtcgcgcgac
      241 acggcggcgt aggcgatgac caccgggttg caggtcacat cgctgccgcg aatgttgtag
      301 agccacgcga tatcgtcgcg cgtcgagatg atgtgccagt cggcgttctt ctcggtcatc
      361 ttggcgcgga gccgtgacag tttcgatgtg cggccttctc ccgcgatatc atcgtcatgc
      421 agtatggcgc ggtccgccgg caggggcggc cggtcggtcc agagagagcc ggcaaggtcg
      481 ccgaccgagc ggagcgctat gcccttcgcg gccatcgcct tctcaagggc gcggaacgcg
      541 ttgatcgaaa aaagcgacgg gtcgatgccg acgacggccc ccgcggccag ttccgaggcg
      601 agccacgcgg tatgggcggg ggtttcgggc agtcccatct tgaagagcgt tatgcccgaa
      661 ccggcgagtt gatcttcggc ctgcaggaaa tagcgcgaat cggtccagag accggccttg
      721 tccttggtca ccacgagcgt cccggcagaa ccgttgaatc cggaaagcca tgcgcgggtc
      781 tgccagtggg cggcgacata ttccgattgg tgcggatcgg tgctcgggac tatgagggca
      841 ttgagcccct gttcggccat ccgggccctc agtagggcga tcttttcgac ggtctgcatc
      901 tttttcctcc gaaggggtta aaaaaacaac agatgattgt agcgtcctta tagggaaata
      961 gcaagaacaa ccgaaaaaag cgggtcaccc gacccgtatc ttctcgaagc gctcccggaa
     1021 gaaggtcgcg aaaacgcgcg aaacgaactc ccgttccgcc ggcgcgatgt cgccggacga
     1081 aaggagcgac cgggccgcct gcgtcgcctt ctgtacctcg atgccgtacc gggccgcaag
     1141 cagatagtac gggtcattgc gaaagcccga ctgttcgagt cccgaaaggg cgccgggccc
     1201 gcgcaggcgc aggtcgaact ccgctatctt gaacccgtcg ctcgtccgtt ccatgaatgc
     1261 gagtcgttcg agcgcctttt cagacacctg ttcgcccgct atgaacgcgg cgaacgaccg
     1321 ttcggccccg cgtcccactc ggccgcgcag ttgatgcagt tgcgcgagcc cgaaccgttc
     1381 cgcgttctcg accgcgatga aggtggcgtc gggcacatcg acgccgacct cgaccacgac
     1441 ggtcgatacg aggaggttcg ccgagccgtc gcggagggcg gcgagcgccg catccttctc
     1501 ctcgcccttc atccggccgt gaagcaggcg tgcccggtcg ccgatcagag cgcggaactc
     1561 ctcgtacact gcggtgaccg gcgctagatc gcttttgtcc gacccttcga cgagcgggca
     1621 tatccagtag cctttcttgc cgtcctcgcg gatgatggaa acgacgtgtt cgatcacctt
     1681 atggcgattg gcgaacggga ccgaacgggt cttcaccggg atgcgccccg gcggcagttc
     1741 gttgagcacc gatatcgaga cgccaccgta gatggtgagc gcgagtgacc gcggtatcgg
     1801 cgtggcgctc gccacgagca catcgggggc ggtcgccttg cccatgagtt ccatccgctg
     1861 cgatacaccg aaccggtgct gttcgtcgat gacgatgagc ccgaggtccg gtagggcctc
     1921 gaggttctcg tagagcgcgt gggtgccgac gatcaggtcc gcgtcgcgga aactgtcgag
     1981 catctccttt ttctttttcc cgctcctgcc gccgacagcc agagccgcct cgaccccggg
     2041 gacgcaccgg gtgatggtat catggtgctg gcgtgccagt atctcggtcg gtgccattat
     2101 gacggcgcgg tatcccgacg cgatgacatc gagcgccgcg aggagcatca cgatggtctt
     2161 gccgctcccg acatcgcctt ggagcaggcg catcatcggt tcgggcgcgg cgaggtcgcg
     2221 ccgtatctcg gcgagaacgc gccgctgggc gttggtcagc gagaagggga gcgccttttc
     2281 gatgtccgcc gcccgtttcg agaaggggtg caccgtacgt ggcttgcgtg gggcggtctc
     2341 gcggcggagc aggtgtgagc cgtactggaa gaagaacatc ttgtcgaacg cgagtcgccg
     2401 gtgatagggg gtgtcgagcg cggcggtctc ggcggcgggg aggtcgctgg cgggaaaatg
     2461 aacattgagt atcgatagga tgaggtcggg ccagtcgtac cggccgcgcg ccaccggcgg
     2521 aagccagtcg ccgtcgaatt tcatgagccg gagcgatgcc tcgcggacgg cacgcaggtg
     2581 gaacaaccgc gacacccccg gtatgcgccg gtagatcggc atgacgcccg acagcagctc
     2641 cttccacacc tcgtcgtacc ggaatatgtc ggggttggtg aaggagggga tgacggtgcg
     2701 gcggctcacc tcgcccgaga cgatgatctc gctcccctcg gggaactgtg ccgcgtagcc
     2761 cttgccgatg gcgccgaaga aactcaccgg tatcttggcg ttcccgatgg ccatctccat
     2821 ggtccacggc gcggtggcgc gccatgccgg cttccatttc atcacggtgc cggtcgccgt
     2881 cgcgatgtcg ccgggggcga cgcccgcgag gtcggcggtc agtatgcggg tctcgtagcg
     2941 ttcggggagc gtctcgacaa ggagatggaa aacggtgtcg ccgagctcct tcttcatctt
     3001 gcgcagttgc ccggtcgaaa gcggcacgac cgactcgatg tcctgcgcga ggaacgacac
     3061 tagtttctcg cccgcggcta ggaaatgggc cgggtcgccg gcgagcaggc ggctcaccag
     3121 cgggaagagc gagagtatcg gttcttcgcg gagcagggcg gtccacgggg gtaatgcttc
     3181 acgcaggtcg gctcggacaa ggcgggtctc gcccttgagg atgaacgcta tctcccgggg
     3241 ggtgagattc cgttcccgcg cgtaggccgt tatagccgcg gcgggtatat cagatgaaat
     3301 tgaccgagac gatctcatat ttccgcgcac cggacggggt ctggaccgtt acctcgtcgc
     3361 cgacctcgcg gccgatgagg gcgccgccga tgggcgagcg gaccgatatc ttgccc
//
LOCUS       DAOQUE010000310         3416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31694_length_3416_cov_3.667658, whole genome shotgun sequence.
ACCESSION   DAOQUE010000310 DAOQUE010000000
VERSION     DAOQUE010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31694_length_3416_cov_3.667658"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..1718
                     /locus_tag="PKH10_09360"
     CDS             336..1718
                     /locus_tag="PKH10_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28371.1"
                     /translation="MRTTATLLTALAVMVMAALIYSCSTEDPVSGGCITAFDCEDGYS
                     CIGGECVNNGGGGDTDKVDDADLPVGDDGEAPDETPDETTEEEGPEEDGLTVDDDQPV
                     GEGDETPDMVTDADVDDAALCSGVTCGGHGTCKVEGGVAVCQCYEGYQDKDGDNVCLV
                     NCANSGLNCGTATHDKCDDSSGMPKCVCDPVADYQDNDENGTCLPTCAKAGLTCTGGT
                     CDDSTGTAKCTCTDTNYQDNDNNGTCEPTCAFANPSCALHSHCDDAGGTATCVCDDGY
                     ITPPACTTCDTNFHSDGSGNCLPNQGCQPNNADCNGHGTCSDSGGVPVCSCFTGYTGT
                     YCTTCATGYQDQSVPGTCLPDCNASCAQNPLLVCPDSHGHCDYSGGSAVCVCDQGWEN
                     PSIILPVNGTFCFGEQASPAPECSDCKISDPPPEYLATGCPADCDSNPVITCTGDCYY
                     HTDGEKYCKP"
     gene            1788..2660
                     /locus_tag="PKH10_09365"
     CDS             1788..2660
                     /locus_tag="PKH10_09365"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNT28372.1"
                     /translation="MIITCPRCDSLLSVPMPGPHQCKHCGAVIKVGDPLKDEKDLLLE
                     GENSGPVETPLSSDGVTRRWREGEGESEEEQDDAQESFDDILAPPRPLSGVAWDRMAE
                     LGFADALYQTTRDIITAPERFFTDMKEAAGPRFIPLYGILFSIGGAALNLFWVLWIIR
                     HHRPIVETFVPAQFLDLMSGMGTADIVAQVFLSPVVNIIVAAFILFGFSILFGARAHL
                     HHFYRLVGFTAVADLLYGIPIIGWIVAFLWRMVLLVKGVRILTDLSPVRAALVFVLYL
                     AISFILLIPTGLGG"
     gene            2663..3142
                     /gene="ispF"
                     /locus_tag="PKH10_09370"
     CDS             2663..3142
                     /gene="ispF"
                     /locus_tag="PKH10_09370"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNT28373.1"
                     /translation="MSFKIGIGFDVHRFVAGRPLYLGGVEIPHDRGLLGHSDADVLLH
                     AVTDAVLGAAGIGDIGELFPDSDPRYKGVRSTLLFEEAMRRVLAAGWRVVNCDAVIVC
                     EEPKILPHRAAIRASLAGLLGVPEDAVMVKGKTSEKLGFTGRGEGVAAQAAVLLERR"
     gene            3147..>3416
                     /locus_tag="PKH10_09375"
     CDS             3147..>3416
                     /locus_tag="PKH10_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28374.1"
                     /translation="MRLPVRFVLGAKNLYLFLLSSVVLLYGLYTYLGEALFYFHDPVP
                     VELGTALEPNKDALETLSDGDYVTIRGIRSVQGGVIEEGFSKKKFM"
BASE COUNT          668 a         1000 c         1033 g          715 t
ORIGIN      
        1 ccatcatcat caggaagcag ttcatcggtc atctcatcag ggagaatggt gtcaaggtca
       61 gataaggcgg tgttgtcgtg gtcaacgaca tcagtatcat tctttctctt aatcgaactg
      121 tcgttacagg aggtgacgag aaagaaaaac gccacgagaa cgatgcgagc gaagaacata
      181 ggaacctccc ttgtcgtgga ggaaccattc tagcacggga gattcattat tcaagaaaag
      241 ccggttttcg ctaaaaacct cttgcaaaaa cgaaacgccg tgttaccata gccgtcgttc
      301 ccggcactga aacgacagcg cgtggaggaa ggacgatgcg gacaacggca accctgttga
      361 cggccctcgc ggtcatggtg atggcggcgc tcatatattc ctgtagtacg gaagaccccg
      421 tttcgggcgg ctgtatcacg gcgttcgact gcgaggacgg ctattcctgc atcggcggtg
      481 agtgcgtgaa taacggtgga ggcggtgaca ccgataaggt ggacgatgcc gaccttcccg
      541 tcggcgacga cggcgaagcg cccgacgaaa cgcctgacga gacaaccgaa gaggaggggc
      601 cggaggagga cggtttgacg gtcgatgacg atcaaccggt gggggagggc gacgagacac
      661 ccgatatggt gaccgatgcc gatgtcgatg atgccgcgct ctgttccggg gtcacctgcg
      721 gcggccatgg tacctgcaag gtcgagggcg gtgtggcggt ctgccagtgt tacgaggggt
      781 atcaggataa ggacggcgac aatgtctgtc tggtgaattg cgcgaactct gggctcaact
      841 gcggtacggc gacgcacgat aaatgcgacg attcctccgg tatgccgaag tgcgtctgcg
      901 atccggtcgc cgattatcag gacaacgacg agaacgggac ctgtctgccg acctgtgcca
      961 aggcgggtct cacctgcacc ggcggtacct gtgacgattc gaccgggacc gcgaaatgca
     1021 cctgcaccga tacgaactat caggacaacg acaacaacgg tacctgcgag ccgacctgcg
     1081 cgttcgcgaa cccttcttgc gcgctccact cgcattgcga cgatgcgggc ggcaccgcga
     1141 cctgcgtctg tgacgacggc tacataaccc cgccggcctg tacgacctgc gataccaatt
     1201 tccattcgga cgggagcggg aactgtctgc cgaaccaagg gtgtcagccg aacaatgccg
     1261 actgtaacgg tcacggcacc tgttccgatt cgggcggcgt gccggtctgc tcctgcttca
     1321 ccggctatac cggaacctac tgcactactt gtgcgaccgg ctatcaggac cagtcggtgc
     1381 ccggcacctg tttgcccgat tgtaacgcca gttgtgccca aaatcccttg ctggtatgtc
     1441 cggatagcca cggtcactgt gattattctg gtggctccgc cgtctgtgtc tgcgatcaag
     1501 ggtgggagaa cccctccatc atcctcccgg tgaacggcac cttctgtttc ggtgagcagg
     1561 catcacccgc tcccgaatgt agcgattgca aaatatctga ccccccacct gaatatctgg
     1621 ccaccggttg ccctgccgat tgcgattcca acccggtgat aacatgtacg ggtgattgct
     1681 attaccacac cgacggagaa aagtattgca agccataaag accacatgtc gccgacgaaa
     1741 tctacgaata tcggatctat gaccgctctc tttcccggag atctgccgtg ataatcacct
     1801 gtccccgttg cgattcgctt ctctcggtcc ccatgccggg tcctcaccag tgcaaacact
     1861 gcggcgctgt catcaaggtc ggtgacccgc tgaaggatga aaaggacctt cttctggagg
     1921 gggagaacag cgggccggtc gaaacgcccc tgtcctccga tggcgtgacg cggcgatggc
     1981 gcgaaggtga gggcgagagc gaagaggaac aggacgacgc tcaggagtcg ttcgacgata
     2041 tacttgcccc gccccgcccc ctctccggcg tggcgtggga ccggatggcg gaactcggat
     2101 tcgccgatgc actgtaccag accacccgag atatcatcac ggcgcctgaa cgcttcttca
     2161 ccgacatgaa agaggcggcg gggccgcgat tcatcccgtt gtacggcatc ctgttcagca
     2221 tcggcggcgc ggcgctgaat ctcttctggg tgctctggat catccgacat caccgtccga
     2281 tcgtcgagac cttcgtgccg gcgcagttcc tcgacctgat gagcggcatg ggcaccgccg
     2341 atatcgtggc gcaggtgttc ctttctccgg tcgtcaacat catcgtggcc gccttcatac
     2401 tcttcgggtt ctcgatattg ttcggcgctc gggcgcatct ccaccatttc taccggctcg
     2461 tcgggttcac cgccgtggcg gatctgctct atggcatccc gatcatcggt tggatcgtcg
     2521 cctttctgtg gcgcatggtc ctgctcgtca aaggggtacg gatactcacc gacctttcgc
     2581 cggtccgcgc ggcgctcgtt tttgtcctgt atctcgccat ttcattcata ctgcttatcc
     2641 ccaccgggct cggaggttga tcatgtcgtt caagatcggc atcggtttcg atgtgcaccg
     2701 tttcgtcgcg gggcgaccgc tctatctggg tggtgtcgag atcccgcatg accgtggcct
     2761 gctcggccat tcggatgccg atgtgcttct gcacgcggtg accgatgcgg tgctcggcgc
     2821 cgccggtatc ggtgacatcg gagagctttt ccccgacagc gacccgcgct acaaaggggt
     2881 acgaagcacc ctcctgttcg aagaggcgat gcggcgtgtc ctcgccgccg gctggcgcgt
     2941 ggtgaactgc gatgcggtca tcgtctgtga ggagcctaag atactaccgc accgtgcggc
     3001 gatacgggct tcgctcgcgg gcctgctcgg cgtgccggag gatgcggtca tggtcaaggg
     3061 taaaacgagt gagaaactcg gcttcaccgg acgcggcgaa ggtgtcgccg cgcaggcagc
     3121 cgtgcttttg gagcggaggt gagcgcatga gactgccggt ccgtttcgtg ctgggggcga
     3181 agaacctcta cctgttcctc ctttcttcgg tggtcctgct gtacggtctc tacacctacc
     3241 ttggcgaggc gctcttctat ttccatgatc ccgtgcccgt cgagttaggg accgcccttg
     3301 aaccgaataa ggacgcgctc gaaacacttt ccgatggtga ctatgtcacg atacggggca
     3361 tccgatcggt acagggcggc gttatagagg aagggttctc gaagaaaaag ttcatg
//
LOCUS       DAOQUE010000311         3411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31756_length_3411_cov_3.885876, whole genome shotgun sequence.
ACCESSION   DAOQUE010000311 DAOQUE010000000
VERSION     DAOQUE010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31756_length_3411_cov_3.885876"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PKH10_09380"
     CDS             <1..364
                     /locus_tag="PKH10_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28375.1"
                     /translation="PYLWWGLAVLVAGGFSIAAGIMQDVDAAKKYNDYRKLSSEEGIA
                     DFIAAGGTREEYVKKTNGLLDEGDASRYGSIITYAFGAALAATGVTLMVWWKDTPVEQ
                     VSVAPTQGGAILTLSGSF"
     gene            374..1033
                     /locus_tag="PKH10_09385"
     CDS             374..1033
                     /locus_tag="PKH10_09385"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT28376.1"
                     /translation="MRYALLLIAFFAISCAQRPDAVDAAEAYNNWMAAHPDEDIDNPA
                     EVESDIDDPYEPDEDIDDLDLPHDPDLHEEDIDMIDEDLVDEDLIINPMTCNADTCDD
                     PYSGLTWQKNSSYTGTQGNSASFCTGIDGFGGFMNWRLPTIAELKTLRRNCDNDAGCA
                     DAAVCYWDVALAGACDKPHWSSTLAGGTQYSTLNFKTGKVAAVESNVDQYARCVTDTT
                     K"
     gene            complement(1088..2806)
                     /locus_tag="PKH10_09390"
     CDS             complement(1088..2806)
                     /locus_tag="PKH10_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNT28377.1"
                     /translation="MAPRVWLVPLFVAFLVVLVASCSSAKDDSNVVPNNDDNSTTDNT
                     TTDNTNVDNNTQPDTASDTTTTDDTVPDEKADNPMTDTDTTVTDEDVAVDTETPEEEA
                     SPVDDAVVPDADEEVPEDENPFIFTAIEPESTFGIDVDTASYSIVRRNLTYNKYMPLA
                     TNVRIEELINYFDYTYTAPAPGADHPFSVTVEMGLCPWYAPHDLLMIGLKGEQLTERM
                     PSNLMFLIDVSGSMEPADRLPLLKTALLTLVDNLGDDDSIGIIKFSDAATEALAPTKA
                     TASGKVTIRAAVNSLTAGGSTNGGAGIQMAYTEAEKNLDESGNNRVILATDGEFNTGG
                     FTTTEQLTALIKAERDKKIFLTVLTFGDPNFIADGNMEALADTGNGNYYYIDSLEEAH
                     RVFTDRLAGTIYTIAKDVKVQVEFNPSKVLKYRLIGYDNRVMENSEFNDDKVDSGELG
                     AGHTVTAFYQIEYVDGMSPFAYAPGADDFVEVRVRYKPPLSDESTMMAVPVTAEHYLA
                     EPSENFRFASAVTEWGLLLRDSYYKFDASYDGAKTRAAGSLGKDPHGLRAQFVTLIDL
                     AKEYDN"
     gene            complement(3096..>3411)
                     /locus_tag="PKH10_09395"
     CDS             complement(3096..>3411)
                     /locus_tag="PKH10_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_148210098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNT28378.1"
                     /translation="TNTVVQCQNERSQHKNRATAMKMLRSKLYELELEKKAAESAEQE
                     KNKMGIDFGSQIRSYVVHPYKMVKDHRFDFETGNAEAVLDGAEILDRFMEDFLLRSSG
                     SK"
BASE COUNT          666 a          989 c          971 g          785 t
ORIGIN      
        1 tccttatctg tggtggggac tcgcggtact tgttgccggt ggcttcagca tcgccgcggg
       61 gatcatgcaa gatgttgatg cggcgaagaa atacaacgac taccggaaac tctcgtcgga
      121 ggaggggatc gccgatttca tcgccgccgg aggaacaagg gaggaatatg tcaagaagac
      181 caacgggctt cttgatgagg gggatgcgtc acggtacggt agtatcataa catatgcgtt
      241 tggcgccgcc ttggcggcga ccggcgtgac cctgatggtc tggtggaaag atacgccagt
      301 cgagcaggtt tcggtcgccc cgacacaggg cggcgcgatc cttacccttt ccggcagttt
      361 ttaggaggcg gccatgagat atgcgcttct tctcatcgcc tttttcgcga tatcctgcgc
      421 ccagcgaccc gacgccgtcg atgcggccga ggcatacaac aactggatgg cggcccatcc
      481 cgacgaagac atcgacaacc ccgccgaggt cgagtccgat atcgatgatc cctacgaacc
      541 tgacgaggat atcgacgatc tcgatctgcc ccacgatccc gacctgcatg aagaggacat
      601 cgacatgatc gacgaggatc tggtcgacga ggacctcatc atcaacccga tgacctgcaa
      661 cgccgacacc tgcgacgatc cctactcggg actcacttgg cagaaaaatt cctcctacac
      721 cggaacgcag gggaactcgg cgtcgttctg caccggcatc gacgggttcg gcggtttcat
      781 gaactggcgc ctgccgacca tcgccgaact gaaaacgcta cgacgcaact gcgacaacga
      841 cgcgggttgc gccgacgccg cggtctgcta ctgggatgtg gcgcttgccg gcgcctgcga
      901 caaaccgcat tggtcttcta cgctcgccgg cggcacgcag tactccaccc tcaacttcaa
      961 aacgggcaaa gtggcggcgg tcgaatcgaa tgtcgaccag tacgcccgct gtgtcaccga
     1021 taccaccaag taattctcta ttaaaaacaa cactcgtttc atatgtcgct tgagtagaac
     1081 ggcaccgtca gttgtcgtac tctttcgcga ggtcgatgag ggtgacgaac tgcgcccgca
     1141 ggccgtgcgg atccttgccg agcgatccgg ccgcccgcgt tttggcaccg tcgtaggagg
     1201 cgtcaaactt gtaataggaa tcgcgaagta gcaggcccca ctccgtcacg gcgctcgcga
     1261 accggaaatt ctccgacggc tcggcgaggt agtgttcggc cgtgacaggc accgccatca
     1321 tcgtgctctc gtccgacagc ggcggcttgt agcgcacccg cacctcgacg aaatcatcgg
     1381 cccccggcgc ataggcgaac gggctcatcc cgtcgacata ctctatctga tagaaggcgg
     1441 tgacggtgtg ccccgccccc agttcacccg aatcgacctt gtcgtcgttg aattcgctgt
     1501 tctccatgac gcggttgtcg tagccgatga gacggtactt gagcactttc gagggattga
     1561 actcaacctg caccttgaca tcctttgcga tggtgtagat ggtcccggca agacggtcgg
     1621 tgaagacgcg gtgggcctct tcaagactgt cgatgtagta gtagttgccg ttaccggtgt
     1681 cggccagcgc ctccatgttg ccgtcggcga tgaagttcgg gtcaccgaaa gtgagcaccg
     1741 tcaggaatat ctttttatcc cgttccgcct tgatgagcgc ggtcaactgt tcggtggtgg
     1801 taaagccgcc ggtgttgaat tcgccgtcgg tggcgaggat gacacggttg ttgccgctct
     1861 cgtcgagatt cttttccgcc tcggtgtagg ccatctgtat cccggcgccg ccattggtcg
     1921 aaccgccggc ggtcaacgaa ttgacggccg cgcggatcgt caccttcccc gacgcggtcg
     1981 ccttggtggg cgccaacgcc tcggtggcgg cgtcgctgaa cttgatgatg ccgatcgaat
     2041 cgtcatcgcc gagattgtcc accagcgtca ggagcgccgt tttgaggagc ggcagacggt
     2101 cggccggctc catcgacccc gacacatcga taaggaacat gaggttcgac ggcatccttt
     2161 cggtcaactg ttcccctttg agtcctatca tcaggagatc gtgcggcgcg taccacgggc
     2221 agagccccat ctcgaccgtc accgagaagg gatggtcggc gcccggcgcg ggagcggtgt
     2281 aggtatagtc gaagtagttg atgagttcct cgatgcgaac attggtggcc agcggcatat
     2341 atttgttgta ggtgagattt cgccggacta tggaataact cgccgtatcg acatcgatgc
     2401 cgaaggtcga ctcgggttca atcgccgtga agatgaaggg attctcatcc tcgggcacct
     2461 cctcgtcggc atcgggaacg accgcatcgt caaccggcga cgcctcctct tcgggcgtct
     2521 cggtatcgac cgccacatcc tcgtcggtca cggtggtatc ggtgtcggtc atcgggttgt
     2581 cggccttttc gtcggggacg gtatcgtcgg tcgtcgtcgt gtctgatgcg gtgtctggtt
     2641 gagtgttatt gtcgacattg gtgttgtcgg tcgtggtgtt gtcggtcgtg ctgttgtcat
     2701 cgttgttggg cacgacattc gaatcatctt tagcagaact gcagctggcc accagaacca
     2761 cgaggaacgc cacgaaaagc ggaacaagcc atacgcgcgg tgccatgata tcctcccccg
     2821 tcggtttaat gatcgtctcc tttcgccgcc tcattatagt ataacgatgc ttggttttct
     2881 cttcgtgatg cgcctcccgt tacgccagac acacttccct tctatagtac ccccgcaaag
     2941 cacggtgtat tataaccgac ggcaaaaaaa ctcaaatagt ttttttcatc tcttttcctc
     3001 aattttttcg cccagccttt tgcctaatag ttctgaaacc ttgcagaata ttacctcgtt
     3061 tattgccctg ttaatgtggt aataaatcga ggtagctact tcgaaccgct tgatctgagt
     3121 aggaaatctt ccatgaaacg gtcgagtatc tcggcgccgt cgaggaccgc ctcggcgttg
     3181 ccggtctcga aatcgaaccg atgatccttt accatcttat aaggatgtac gacataggag
     3241 cgtatctgac tgccgaaatc tatccccatc ttgttctttt cctgctcggc gctctcggcc
     3301 gccttcttct ccaactccag ttcatagagt ttcgagcgga gcattttcat cgcggtcgcc
     3361 cggttcttgt gctgtgaccg ctcgttctgg cactgcacga cggtgttggt c
//
LOCUS       DAOQUE010000312         3405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31833_length_3405_cov_3.596119, whole genome shotgun sequence.
ACCESSION   DAOQUE010000312 DAOQUE010000000
VERSION     DAOQUE010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31833_length_3405_cov_3.596119"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKH10_09400"
     CDS             complement(<1..605)
                     /locus_tag="PKH10_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28379.1"
                     /translation="MRSFLVALLLFATLTGQETPAATETPESTDPPPPRFTGHSSSRV
                     NIHFDHQSTDTAIPDDYTTLAQELFIEGTYDRYTVGMRKNLLLFLGRGPDDKVKNFDY
                     RYAPEAFNHGWMHALDRIYLKMHWPVVTVTAGDFHESMNRGLLFSLLKDPAGEDNALR
                     GGSVTVRSGDFHAKAFGGVANPLLRDRATLERMGEANDILWG"
     gene            complement(613..1167)
                     /locus_tag="PKH10_09405"
     CDS             complement(613..1167)
                     /locus_tag="PKH10_09405"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNT28380.1"
                     /translation="MIRTLFTTLAAAFFLSGACASTDNTGPSPTPVAPPAATPTATDG
                     GFSLDLATWDGGRFSFDDIRGRKHLLVAFWATWCDPCKAELRDLAAIYDRYRDKVEFV
                     AVSTDTEESMDKVRAFVIENGLPFPVLVDPTKARTASLIPGGDTVPYSILVNKSGAVV
                     SRHTGYEPGDGDRTVRELDALPAD"
     gene            complement(1184..1882)
                     /locus_tag="PKH10_09410"
     CDS             complement(1184..1882)
                     /locus_tag="PKH10_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28381.1"
                     /translation="MTKNYLFTTLLFALLLAILPACGDDNDPATDNSPASDESATADD
                     MLVDETPDIDTAEPLPEYPTETPTSNQVDDIAANITFFDHEDVMWTLGHFYKKKKLIW
                     LIFSAYNCPYCNVEKADLPDVYKKEYIDGGLEIIFIENGLLSGPQPSKEPEKLKNMRD
                     AMIDGYGDKAKFVYGYLEMDMQSTLAKYVMAGYPTNALIDATSMKILNYFEGWDSSLA
                     DNQERAIQFWLDEL"
     gene            complement(1907..2671)
                     /locus_tag="PKH10_09415"
     CDS             complement(1907..2671)
                     /locus_tag="PKH10_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNT28382.1"
                     /translation="MKFSCYGKTDVGRKRELNEDSFAVLDEERVFVVADGMGGHNAGE
                     VASSIAIETIVNFFRASKEDEDMTWPYKMDPNLSIEANKLKVGIKFANRKIFRVAQGN
                     ASYSGMGTTIVAMLLLENGPLYIAHVGDSRCYRYADGAISQVTEDHSLVNEYVKAGQL
                     TPEQARNFPHKNVIMRALGMKENVLVDVLERPVSKGEIYFACSDGLSDMVTDDDMAAI
                     LAESKNLREAADKLIDRANANGGKDNISVVLLRIDE"
     gene            complement(2674..>3405)
                     /locus_tag="PKH10_09420"
     CDS             complement(2674..>3405)
                     /locus_tag="PKH10_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28383.1"
                     /translation="GTFHLVITKMIQVWGDRPLPPIAVLRGGTINQVATNVKARRGAL
                     ANLKRLVKKYKDGDEPRIVQKPLFRIDDKYGFIFGNGIVTAFMDYYYEHGDPSPLVAF
                     RTLLNSVGSIVLRTRLYRKLFEGFDAHLVVDGKDLGTKPYLGIFVTSLSQIGLGFEPF
                     WRNRHLTDKMQMLAIHKGGWIVPFLGLAWLGKPLPKSTVLDTIGKHFSIRVGRPFKYT
                     IDGDLYQSAGSLDLFVDRSVDIIAG"
BASE COUNT          598 a          943 c         1138 g          726 t
ORIGIN      
        1 ccccagagga tgtcgttcgc ctcgcccatc cgttcgagcg tcgcgcgatc gcgcaggagg
       61 gggttggcga cgccgccgaa ggccttggcg tgaaagtcgc ccgaccggac cgtgaccgat
      121 ccgccgcgga gggcgttatc ctcgcccgcc gggtccttca ggagtgagaa gagcagccca
      181 cggttcatgc tttcatggaa gtcgcccgcc gtgacggtga cgaccggcca gtgcatcttg
      241 agatagatgc ggtcgagggc atgcatccat ccgtggttga acgcctccgg tgcgtagcga
      301 tagtcgaagt tcttcacctt gtcgtcgggg ccgcgaccga ggaagagcag aaggttcttg
      361 cgcatgccga cggtatagcg gtcataggtt ccctcgatga agagttcttg ggcaagggtg
      421 gtgtagtcgt cgggtatcgc ggtgtcggtg ctctgatgat cgaagtggat gttgacgcgg
      481 ctcgacgagt ggccggtgaa ccgcggtggc gggggatcgg tgctttccgg ggtctccgta
      541 gcggccgggg tctcctgtcc ggtcagggtg gcgaacaaga gcaatgcgac gaggaacgat
      601 cgcatcggcg cgtcagtcgg ccggcagggc gtcgagttcg cggacggtcc ggtcgccgtc
      661 gcccggttcg taaccggtgt ggcgggaaac gacggcgccg cttttgttca cgaggatcga
      721 ataaggcacc gtgtcgccgc cggggatgag cgaagcggtc cgcgccttgg tcggatccac
      781 gagcaccggg aaggggaggc cgttctcgat gacgaaggcc cgtaccttgt ccatgctttc
      841 ttcggtgtcg gtcgaaacgg cgacgaactc gaccttgtcc ctgtagcgat cgtagatggc
      901 ggcgagatcg cgcaattcgg ccttgcaggg gtcgcaccag gtcgcccaga aggcgacgag
      961 caggtgtttc ctgccgcgga tgtcgtcgaa cgagaatcgt ccgccgtccc aggtcgcaag
     1021 gtcgagcgag aagccgccgt ccgttgcggt cggggtggcc gcggggggcg cgaccggcgt
     1081 cggagagggg ccggtgttat cggtggaggc gcaggcgccc gacaggaaaa aggccgcggc
     1141 gagtgtcgtg aaaagggtgc ggatcatgcg gatctccggg ctatcagagt tcgtcaagcc
     1201 agaactggat ggcccgttcc tgattatcgg cgagcgagga gtcccatccc tcgaagtaat
     1261 tgagtatttt catcgaggtc gcgtcgatga gggcgttggt ggggtatccg gccatgacat
     1321 atttggccaa cgtcgactgc atgtccatct caaggtagcc gtagacgaat ttcgccttgt
     1381 cgccgtagcc gtctatcatc gcatcacgca tattcttcag tttttccggc tccttggagg
     1441 gctgggggcc cgagagcagg ccgttctcga tgaagatgat ctcgagcccg ccgtctatgt
     1501 actccttctt gtatacgtcg ggaaggtcgg ctttttctac attgcaatag gggcagttgt
     1561 aggcggagaa gatgagccag atgagttttt tctttttgta gaagtgaccg agcgtccaca
     1621 tcacatcctc gtgatcgaag aaggtgatgt tcgcggcgat gtcgtcgacc tgattggagg
     1681 tcggggtctc cgtcgggtac tcgggaagcg gctcggcggt gtcgatgtcg ggggtctcgt
     1741 cgacgagcat gtcatcggcc gtcgccgact catcggaggc ggggctgttg tcggtggcgg
     1801 ggtcgttgtc gtcgccgcag gccggcaata tcgccagcag gagcgcgaaa aggagcgtcg
     1861 tgaaaagata gttcttggtc atggggatcc tctcctatcg ttaaggttat tcgtcgatgc
     1921 gcaacaggac caccgatatg ttgtccttgc cgccgttcgc gttcgcgcgg tctatcagtt
     1981 tgtcggccgc ttcgcgtagg ttcttcgact cggcgaggat ggcggccatg tcgtcgtcgg
     2041 tcaccatgtc cgacagtccg tcggaacagg cgaagtatat ctcgcccttg ctgaccgggc
     2101 gttccagcac atccaccagc acattctcct tcatgccgag cgcccgcatg atgacattct
     2161 tgtgggggaa gttccgcgcc tgttcgggcg tcagttgccc cgccttgaca tactcgttga
     2221 cgagcgagtg gtcctcggtg acctgcgaga tggcgccgtc ggcgtagcgg taacaccggc
     2281 tgtcgccgac atgggcgatg taaagcggcc cgttctcgag caggagcatc gcgacgatgg
     2341 tggtgcccat gccggagtaa ctcgcgttac cctgcgcgac gcggaatatc ttgcggttgg
     2401 cgaatttgat gccgaccttc agtttgttgg cctcgatcga gaggttgggg tccatcttgt
     2461 aaggccaggt catgtcctcg tcctctttgc tcgcccggaa gaaattgacg atggtctcta
     2521 tggcgatgga actggctacc tcaccggcat tgtgaccgcc catgccgtcg gcgaccacga
     2581 acacccgctc ttcgtcgagt accgcgaagg aatcttcgtt gagttcccgt ttcctgccaa
     2641 catcggtctt gccgtagcaa ctgaacttca tcattacccc gctatgatat caacgcttct
     2701 gtcaacgaac aggtcgagcg agccggcgct ctggtagaga tcgccgtcta tcgtgtattt
     2761 gaacgggcgc cccacccgta tcgagaagtg cttgccgatg gtgtcgagca cggtgctctt
     2821 gggaagcggc ttgccgagcc aggcgagccc gaggaacggg acgatccagc ctcctttgtg
     2881 tatcgcgagc atctgcatct tgtcggtgag gtggcggttg cgccagaacg gctcgaaccc
     2941 gagcccgatc tgcgagagcg aggtgacgaa gatgccgaga tacggtttcg tgccgagatc
     3001 cttgccatcc acgaccagat gcgcatcgaa cccctcgaaa agtttgcggt acagccgggt
     3061 ccgcagaacg atggacccga ccgagttgag aagcgtcctg aaggcgacga gcggggaggg
     3121 atccccgtgc tcgtagtagt agtccatgaa ggcggtcacg atgccgttgc cgaaaatgaa
     3181 cccgtacttg tcgtcaatgc ggaagagtgg cttctggact atgcgcggtt cgtcaccgtc
     3241 cttgtacttc ttgaccagtc ttttcaggtt ggcgagtgcg ccgcgccggg ccttcacgtt
     3301 ggtcgcgacc tggttgatgg tgccgccgcg caggaccgcg atagggggaa gcggccggtc
     3361 gccccagacc tgtatcatct tggtgatgac cagatgaaac gtgcc
//
LOCUS       DAOQUE010000313         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31842_length_3404_cov_4.248134, whole genome shotgun sequence.
ACCESSION   DAOQUE010000313 DAOQUE010000000
VERSION     DAOQUE010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31842_length_3404_cov_4.248134"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKH10_09425"
     CDS             complement(<1..182)
                     /locus_tag="PKH10_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28384.1"
                     /translation="MDEFTWFNRRRHGMPGTEQYYAGNAPGGEGRYDLIKRKIGHDGG
                     SRALFIGLRLPYCTSPL"
     gene            147..494
                     /locus_tag="PKH10_09430"
     CDS             147..494
                     /locus_tag="PKH10_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HNT28385.1"
                     /translation="MSPSIKPGELVHVSPVQHDRLTSGDVVVFRSKDAFVAHRLMWRR
                     GFVARTKGDNNAYGDPPISVFDIVGVVEGKSSRRAAWYYLLRNRIRHYVPLPARRLFS
                     ALKKSMYDRKESL"
     gene            491..1501
                     /locus_tag="PKH10_09435"
     CDS             491..1501
                     /locus_tag="PKH10_09435"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT28386.1"
                     /translation="MTIKRLGILFEIADRCNARCLHCYQPRDCGHRLMSLAEIERVLD
                     ILAGNEYLDVTFSGYEPFTNPDLWDILAAARKRRFYINLKTNGLLLDRDAVQRLKALH
                     VGSVHISLYSADHAEHDRAAGAPGAYDRIMMVLDHLKEAGIRTVLAAPLIHPMPDLAR
                     LTAFAKERDLQLVIDHGIFSSFDKRPEVESVKLTDEELEVALRFQYAVEGGEMDLYAP
                     KERSFLCHYGDPGTLRLDGAGDLFACGKCTVPLGNILTDDFAGMLGKAWAERPPIIER
                     RECNECNLFPYCNPCPAESLLESGSLYACSTNRKRIAELKKRISGLKKSGKPNSSEPC
                     HF"
     gene            1759..2514
                     /locus_tag="PKH10_09440"
     CDS             1759..2514
                     /locus_tag="PKH10_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28387.1"
                     /translation="MRARRFDIADLSWVVSGVGISRFPAPWRLFECAAKGPEQENSIS
                     IVTHDRADLNNLQRVNGVWSIGRHRPYLYLEKVLAYEGFERRELFLLVHSCFLVDRDG
                     GGHLFLARSGTGKSTLARKMRGLMTGGNDETNILYRCGGALLVSSTPFYTIPKLKQAV
                     INNITAPLKQVYLLHKATTGPSRIDDPVDPDALWRYLVEGQVSTPYTKKEYFAAYHRM
                     MNDLCNTIPVRPFFHTLHDSPEQVAMVLRGAAS"
     gene            2596..2967
                     /locus_tag="PKH10_09445"
     CDS             2596..2967
                     /locus_tag="PKH10_09445"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HNT28388.1"
                     /translation="MDEAGAEKYDPDVEKRKSPRIEIELSVFFAVSSELPHNFFTGIM
                     MDLSTGGIFLSTEQMLPVGSRLHLSFLMDTRRVEGHAEVCWTRKAAPGVEPGMGLRFL
                     DIPGEDRDLIEFYIRQKKPIG"
     gene            3076..>3404
                     /locus_tag="PKH10_09450"
     CDS             3076..>3404
                     /locus_tag="PKH10_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT28389.1"
                     /translation="MRKAEYAVSLPGGKVRCELCPQRCTLSDGQTGVCKVRRNIKGTL
                     YSLVYGRPVALNNDPVEKKPLFHFLPGTKTLSIATAGCNMNCNFCQNWEISQATPDSV
                     NSYDLTPA"
BASE COUNT          719 a         1010 c          959 g          716 t
ORIGIN      
        1 aggggggatg tacaatacgg caagcgaagg ccaatgaata aggccctgct ccctccatca
       61 tgtccgatct ttctcttgat cagatcatag cgaccttctc cgccgggggc gttcccggcg
      121 tagtattgtt cggttcccgg cattccatgt cgccttctat taaaccaggt gaactcgtcc
      181 atgtctcacc ggtacagcac gaccgcttga cgagcggcga tgtggtggtg ttccgttcaa
      241 aggacgcttt cgtggctcat cggttgatgt ggcggagggg tttcgtggcc cgcacaaaag
      301 gcgacaataa cgcctacggc gacccgccga tatcggtgtt cgacatcgtt ggggtggtgg
      361 agggtaaaag ttcgcgccgc gcggcgtggt actacctcct gcggaaccgg atccgtcact
      421 atgtgccgct tccggcgcgt cgactgtttt cggcgctgaa aaagagcatg tacgaccgga
      481 aggaatcgct atgacgataa agcggctcgg tatcctgttc gagatcgcgg accggtgcaa
      541 cgcgcggtgt cttcactgct accagccacg ggactgcggc caccggctca tgtcgctcgc
      601 cgagatcgag cgggtgctcg acatcctcgc cgggaacgag tatctcgatg ttaccttcag
      661 cggctacgag cccttcacca atcccgatct gtgggatatc ctcgcggcgg cgcggaagcg
      721 ccggttctac atcaacctga agaccaacgg gctcctgctt gatcgcgacg cggtgcaacg
      781 actgaaggcg ctgcatgtcg ggtcggtcca tatctcgctg tattcggccg atcacgccga
      841 acacgaccgt gccgccggtg cccccggggc ttacgaccgc atcatgatgg tgcttgatca
      901 tttgaaagaa gcggggataa gaacggtgct cgccgcgccg ctcatccatc cgatgcccga
      961 tctggctcgg cttaccgcat tcgctaaaga gcgcgacctg caactggtca tcgatcacgg
     1021 catcttcagt tcgtttgaca agcggcccga ggtcgagtcg gtgaaactga ccgacgaaga
     1081 actcgaggtg gcgctccgat tccaatacgc ggtggaaggc ggggagatgg acctctacgc
     1141 accgaaagag cgctcctttc tttgtcatta cggcgatccc ggaacgctcc ggctggatgg
     1201 cgccggggac ctttttgcct gcggcaagtg taccgtgccg ctcggcaaca tactgaccga
     1261 tgactttgcc ggtatgctgg gcaaggcgtg ggcggaacgg ccgcccatca tcgaacggcg
     1321 ggaatgcaac gagtgcaacc tgttcccgta ctgcaatccc tgccccgccg aatcgcttct
     1381 cgaaagcggc tcgctgtacg cctgttcgac gaaccgtaaa cggatagccg aacttaaaaa
     1441 acgaataagc ggactcaaaa agtccggaaa acctaactct tcggaaccgt gtcattttta
     1501 ggaagtaaaa gaaatcgctt gactttttac gctcttttcg ttacctttat ccgaaattaa
     1561 gggagaaaga aggcatatat gggatatgaa aagccggcgc tgatagaaga aatagccctt
     1621 cctagggaat tgcttttcaa atgtaacatg atggactgtc agtgtacggg acaatgttcc
     1681 gattatcgca gcgacatcat ggaacataaa tgccctaaac cggacaggcc cccttgtgtc
     1741 tgaagacaga aacaaagcgt gagggcgcgg cgcttcgaca tagccgacct ctcttgggtc
     1801 gtttccggag tcggtatttc ccgcttcccc gcaccgtggc ggctttttga gtgcgcagcg
     1861 aagggcccgg aacaagagaa ctccatatcg atagtcacgc atgaccgcgc cgatctcaac
     1921 aatctgcaac gggtgaacgg tgtctggtct atcgggcgac accggccgta tctttatctg
     1981 gagaaggtgc ttgcgtacga ggggttcgag cggcgggagt tgttcctgct cgtccattca
     2041 tgttttctcg tggaccgcga tggtggcggg catctgttcc tcgcccgttc ggggaccggc
     2101 aaaagcacgc tcgcgcggaa gatgcgcggc ctcatgaccg gcggcaacga cgagacaaac
     2161 atcctctacc ggtgcggcgg tgcgctgctg gtttcttcca cccccttcta cacgataccg
     2221 aaactcaaac aggcggtcat caacaacatc accgcgccgc tcaaacaggt atatctcctg
     2281 cataaagcga cgacgggccc gtcgcgtatc gacgatccgg tcgaccccga cgcgttgtgg
     2341 cgttatctcg tcgaagggca ggtgtccacc ccctacacga agaaagagta tttcgccgcc
     2401 tatcaccgca tgatgaacga tctctgcaac accattccgg tccgcccgtt cttccacacc
     2461 ttgcatgata gccccgaaca ggtcgcgatg gtgttacggg gggccgcatc ctaaaaacat
     2521 cggccctctt gttgctttcc ttttcttgaa tgattataat acctccagct catcaaccat
     2581 ggaaggggga ataacatgga cgaagccggc gcggaaaagt atgatccgga tgttgagaag
     2641 aggaaaagtc cccgcatcga gatcgaactg tcggtcttct tcgcggtgtc atcggagctg
     2701 ccgcacaatt ttttcaccgg tatcatgatg gacctcagta ccggcggcat cttcctttcc
     2761 accgaacaga tgctccccgt cggttcccga ctgcacctgt cgttcctcat ggacacccga
     2821 cgggtcgagg gacatgccga ggtctgctgg acacgcaagg ccgcgccggg cgtcgagccg
     2881 ggcatggggc tccggttcct cgatatcccg ggcgaggatc gcgacctcat cgagttctat
     2941 atccggcaga aaaaaccgat cggctgaaca tcccccgtgg accgaaggat atcctccgcg
     3001 ctgatcctcc ttatcctgac gctgaccccg acattcttct acctttcggg tcgttcgccc
     3061 gacgaggtgt cgctcctgcg caaggcggag tatgcggtgt cgcttcccgg cggtaaggtg
     3121 cggtgtgaac tctgtccgca gcgttgtacg ctttcagacg gacagaccgg cgtctgcaag
     3181 gtgcgccgca acatcaaggg gacgctctac tcactcgtct acggcaggcc ggtcgcgctc
     3241 aacaacgatc cggtcgagaa aaaaccactg ttccatttcc tgcccggcac taagaccctc
     3301 tccatcgcga ccgccggatg caacatgaac tgcaacttct gtcagaattg ggagatatcc
     3361 caggcgacgc ccgacagcgt caactcctac gacctgaccc cggc
//
LOCUS       DAOQUE010000314         3398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31922_length_3398_cov_4.177386, whole genome shotgun sequence.
ACCESSION   DAOQUE010000314 DAOQUE010000000
VERSION     DAOQUE010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31922_length_3398_cov_4.177386"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKH10_09455"
     CDS             <1..298
                     /locus_tag="PKH10_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018300225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNT28390.1"
                     /translation="GRVAATSPVLRRFMQETAKETGMLTNIRSIGMIAAADIKPEFIK
                     APRTGYRVYRRAIELGALLRPLGNTIYWLPPLNTSVETLVELKEITVRALKEAL"
     gene            complement(340..1284)
                     /locus_tag="PKH10_09460"
     CDS             complement(340..1284)
                     /locus_tag="PKH10_09460"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNT28391.1"
                     /translation="MQVYRDLCAAPGGSRYSVVIGNFDGLHIGHRAIFDEARRREGAL
                     AVITFDPHPRAVLQPGYAPRMLTSFEERLAFFDALGVASVFALPFEPLRSLAPVEFLD
                     LLHRSIPLAAVTVGFNFNFGRGQEGNTDILLWWSKGSGVAAHIVPPVVHRGTRVSSSA
                     VREFIMNGEMERAAERLVFPYVMTGTVRRGRQVGAQLGFPTLNLPVPDKLLPPDGVYA
                     TALVMNGARYGAVTNIGTNPTMDAETGERTIETHVPGVTLPERYDERVAVYFLSRIRR
                     EIRFSSREKLREKITQDIAIAQERLAGIDLKGLPELTS"
     gene            complement(1284..2279)
                     /locus_tag="PKH10_09465"
     CDS             complement(1284..2279)
                     /locus_tag="PKH10_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HNT28392.1"
                     /translation="MAVSYRELGLTNTREMFKKAMAGKYAVPAYNFNNMEQLQAIIQA
                     CVETRSPVILQVSSGARKYADATLLRYMAKGAVEYAKKLGVEIPITLHLDHGDTFELC
                     KDCVDNGFSSVMIDGSHHPYEKNVELTRQVVEYAHKFDVTVEGELGQLAGIEDEVSAE
                     HSTYTRPDEVVDFVSKTGVDSLAISIGTSHGATKFKPEQCTLSPDGVLIPPPLRFDIL
                     EEIEKRLPGFPIVLHGSSSVPVEQVKLINQYGGKMKDAVGIPEEQLRRAAASAVCKIN
                     IDSDGRLAMTAAIRKVFAEKPSEFDPRKYLGPARDALKELYKHKNKSVLGSADRV"
     gene            complement(2281..3105)
                     /locus_tag="PKH10_09470"
     CDS             complement(2281..3105)
                     /locus_tag="PKH10_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT28393.1"
                     /translation="MRDLRKDRVVAGFSRAAATYDDAAVVQRRNAEHLAALVRGHIAD
                     PPSLAPFLEIGAGTGLLTDRLLDMGFARGTVTDVAPAMVDTLKKRFDGRPGLTVRELD
                     GERFTVGERFGAIVSASTFQWFSSLLDPLRNMRRHLNDWGLLAFCLFVDGTFRELRQA
                     ANAVGCPYPGHRLFEANEVARAMLDAGFTVDHFASFDTPVRYPDALRFLRSVRHIGST
                     NALPGPTAPGELRRIIRRYDDDHRCADGVTATYTTLLVLGHKRSAPRPMNNHNQGG"
     gene            complement(3098..>3398)
                     /locus_tag="PKH10_09475"
     CDS             complement(3098..>3398)
                     /locus_tag="PKH10_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28394.1"
                     /translation="LARFRLDIGPSLPTDPATLLAQLAFLENYRVPRELPAVPVTILH
                     GGRDAIVDAAAVAAWRQLIPGAAARLVAEAGHALPFVRRDEVVAAIAALREACDA"
BASE COUNT          597 a         1087 c         1115 g          599 t
ORIGIN      
        1 cgggcgggtc gccgcgacct caccggtgct gcgccgcttc atgcaggaga cggcgaagga
       61 aacgggaatg ctcaccaaca tccgcagcat cgggatgatc gccgccgccg acataaagcc
      121 ggaattcata aaagcgccgc gcaccggcta ccgggtctac cgccgcgcga tagagctcgg
      181 cgcgctgttg cggccgctcg gcaacaccat ctactggctg ccgccgctca atacttcagt
      241 ggaaactctc gttgaactca aagaaataac ggtcagggcg ctgaaagaag ccctgtaggt
      301 attccttcca cccccttgtc aaagggggtt tgacgggatt tatgatgtga gttccggcaa
      361 tcctttcaga tcgatcccgg cgagccgttc ctgtgcgata gcgatgtcct gcgttatttt
      421 ttcccgcagt ttttcgcgcg aactgaaccg gatctcgcgc cgtatccgcg acaggaaata
      481 gaccgcgacg cgctcgtcgt acctttcggg cagggtcacg cccgggacat gggtctctat
      541 cgtccgttcg cccgtttccg catccatcgt cgggttggtg ccgatattgg tgacggcgcc
      601 gtaccgtgcg ccgttcatca ccagcgccgt cgcatagacg ccgtcgggcg gcaggagttt
      661 gtcgggcacc ggcagattga gcgtcgggaa gcccaactgc gcgccgacct gccggccgcg
      721 ccggaccgtg ccggtcatga cataggggaa cacaagccgt tcggccgccc gctccatctc
      781 gccgttcatg atgaactcgc ggacggcgct cgaactgacc cgcgtcccgc ggtggacgac
      841 cggcggaacg atatgcgccg caacgccgct ccccttgctc caccacagca ggatgtcggt
      901 gttcccctcc tgtcccctgc cgaagttgaa gttgaagccg accgtgacgg ccgcaagcgg
      961 gatcgaacgg tggagcaggt cgaggaattc gaccggcgcg agcgaccgga gcggctcgaa
     1021 gggaagcgcg aagaccgacg cgacgcccag cgcatcgaaa aaggcgagcc gctcttcgaa
     1081 cgaggtcagc atccggggcg cgtaaccggg ctggagcacg gcgcgggggt gcgggtcgaa
     1141 ggtgatgacc gcgagcgccc cttcgcgccg ccgcgcttcg tcgaatatcg cgcggtgtcc
     1201 gatgtgcagg ccgtcaaagt tgccgatgac cactgaatag cggctaccgc cgggcgccgc
     1261 gcaaagatcg cgatatacct gcatcagacg cgatcggccg aaccgaggac gctcttattc
     1321 ttatgcttgt agagttcttt cagcgcatcg cgggcggggc cgagatattt gcgcgggtcg
     1381 aactcggagg gcttctcggc gaataccttg cggatggccg ccgtcatcgc aagccgcccg
     1441 tcggagtcga tgtttatctt gcagaccgcc gacgcagccg ccctgcgcag ttgctcttcg
     1501 gggatgccga cggcatcctt catcttgccg ccgtactggt tgatgagttt cacctgctcg
     1561 accggcaccg atgaggagcc gtgcaggacg atcgggaagc cggggagccg tttctctatc
     1621 tcttcgagta tgtcgaaccg cagcggcggc gggatgagca ccccgtcggg cgacagcgta
     1681 cactgttccg gtttgaactt cgtcgcgccg tgcgaggtgc cgatcgatat cgcgagcgaa
     1741 tcgacgccgg tcttcgacac gaagtcgacc acctcgtcgg gccgcgtgta ggtggagtgc
     1801 tcggccgaca cctcgtcctc gatgcccgcc aactgaccga gttcgccttc gacggtgaca
     1861 tcgaacttgt gggcgtattc taccacctgc cgggtcagtt cgacattctt ttcgtaggga
     1921 tggtgcgacc cgtctatcat gaccgacgag aagccgttgt cgacgcagtc cttgcacagt
     1981 tcgaaggtgt cgccgtggtc aaggtggagc gtgatcggta tctcgacacc cagtttcttg
     2041 gcatactcga ccgccccctt ggccatgtag cgcaggagcg tagcgtcggc gtacttccgg
     2101 gcgcccgacg agacctgcag gatgaccggc gaccgggtct cgacgcaggc ctgaatgatc
     2161 gcctgcaact gctccatgtt gttgaaattg taagcgggaa cggcatactt gcccgccatc
     2221 gcctttttga acatctcgcg ggtgttcgta agtcccagtt cacgataact gaccgccatg
     2281 tcatcctcct tggttatggt tgttcatagg tcgtggcgca cttcgtttat gtcccagcac
     2341 caacagcgtc gtataggtcg cggtcacgcc gtcggcgcac cgatggtcgt cgtcgtagcg
     2401 gcggatgatc ctgcggagtt cgcccggggc ggtcgggccc ggcagggcgt tcgtcgatcc
     2461 gatatgccgc accgaccgca ggaaccgcaa ggcgtcggga tagcggaccg gcgtatcgaa
     2521 actcgcgaaa tggtccaccg tgaacccggc gtcgagcatc gcgcgcgcca cctcgttcgc
     2581 ctcgaacagg cgatggcccg gataggggca cccgacggcg ttcgccgcct gccgcagttc
     2641 gcggaaggtg ccgtcgacga acagacagaa ggccagcagt ccccagtcgt tcaggtgccg
     2701 ccgcatgttc cggagcgggt cgaggagcga tgagaaccac tggaaggtgg atgccgacac
     2761 tatcgcgccg aaccgttcgc cgaccgtgaa ccgctcgccg tcgagttcgc gcaccgtaag
     2821 cccggggcgg ccgtcgaatc gttttttgag cgtatcgacc atcgcggggg cgacatcggt
     2881 caccgtgccg cgcgcaaagc ccatgtcgag cagacggtcg gtcaacagcc cggtgccggc
     2941 gccgatctcg aggaacggcg cgagcgacgg gggatcggcg atatggcccc gcacaagggc
     3001 ggcgaggtgt tcggcgttgc gtcgctggac gaccgcggcg tcatcatagg tcgccgcggc
     3061 gcgcgagaag ccggcgacga cccggtcctt gcgcaggtca cgcatcgcat gcctcccgca
     3121 gagcggcgat ggccgcgacg acctcgtccc gccgcacaaa gggcagggcg tgccccgcct
     3181 ccgcgacaag gcgcgccgcc gcgccgggga tgagttgccg ccacgccgcg accgccgcgg
     3241 catcgacgat ggcgtcccgc ccgccgtgca gtatcgtcac cggcaccgcc gggagttcgc
     3301 gcgggacacg gtagttctcg aggaaggcaa gttgcgcgag gagcgtcgcc ggatcggtcg
     3361 ggagcgacgg cccgatatcg aggcggaaac gggcgagc
//
LOCUS       DAOQUE010000315         3397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_31935_length_3397_cov_4.745063, whole genome shotgun sequence.
ACCESSION   DAOQUE010000315 DAOQUE010000000
VERSION     DAOQUE010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31935_length_3397_cov_4.745063"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..642)
                     /locus_tag="PKH10_09480"
     CDS             complement(103..642)
                     /locus_tag="PKH10_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28395.1"
                     /translation="MKERLIASFATLSSREKNILVVFGTALILFAVAATFLFFDDAVD
                     TRREEIAERRSLMEKVTVHREAFRRAKDREAATLLAIQNNETNLNSYLNGVRETYGVE
                     ISTIKELKAEKKGEFQKEMIEVGIRSIDMDALMSFLYALENKARFVFLPSISIKKRFD
                     GKNYEVTMIVATLKEAAEK"
     gene            complement(639..2252)
                     /locus_tag="PKH10_09485"
     CDS             complement(639..2252)
                     /locus_tag="PKH10_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28396.1"
                     /translation="MKCAVCRIQQDRVVMLEFEREYNRLAAQRFTVCSFEEAPAALAQ
                     YDHIDIVVPAAATLYLASAYPTVPRPALERIVAQDIEATTPFKESEVVFDLAPLRSDG
                     IVPVFVAQKKDLHAIIDRSGAPLRDRVRSLIPETALVIGEGAGMQWLFIDDDQIVLFG
                     ERGQVVRTTGLSRLVAMMRSSLGGAKTEEEVLQTLAASMDLSDGPALSEDELLMRKAT
                     EQFFQEMVADLGRFMRRDRRTVVCFADRLPGNAEKLIASVIPDAMVISAEDALARIFL
                     RAEQGESPSFSRGEFVYRGGFNFLKKRLLLAAALFALAFVLMITAFELRLWYLDRALD
                     ALDEQARTVTKEILGKEYPSLRQALSVMQKTIGGGGKDGKDGKDAKGDKRLYPYSSLF
                     IMEQVFPLASFDGSTIELRDLSIKDGKVKLTGEAESLDHINTLLEQLEKMEYATELNK
                     GQITSRGGKNSFNIGFTFQKRSAKEHAKKEKDKGKEKEKEKVSPTEQADGDAPKNVPP
                     PAERAEPSRSPARGPEPAGKETVPWEEEL"
     gene            complement(2265..3365)
                     /locus_tag="PKH10_09490"
     CDS             complement(2265..3365)
                     /locus_tag="PKH10_09490"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNT28397.1"
                     /translation="MLPIVTDMNYEARTEFDLAMNYKKKAESQVLAQSGLTFAAIVFD
                     LQKQIEGLLKSSGLKELGLQSFEIWDIVPFDTSILRAFSGADTFADTDSILSAEEGTA
                     VTGKEEGTVAPVVDESDPLFDFPGDFKLEFENEDTKININLLDSAAKETVARMLWGLV
                     EPQVYDFLFLENTSRREFVTREELIANIADWVDGDDERYLIGGDERSMYEEFSPRYEV
                     KNARFDTVDELRMVYGVDEVAFRLLAPFVTIYSSGRINVNKAHPIILESIIRSYAKDK
                     NQSMFYNEDIMREFMGKVLARRARDGFYKVDDFITAVREEGVELEPAVASQLSAEGSF
                     YRIRVRGIRDGIESYIEVVSDKNFNIYFYREG"
BASE COUNT          634 a         1063 c          928 g          772 t
ORIGIN      
        1 cgctccgagc agccgcggcg agccgatcat cgctgaacaa ccggtcctcg atcgttcgca
       61 acaggggcca caggtcccgc aggatcgata gtatccgttc cttcattttt cagcggcctc
      121 cttcagcgtc gcgacgatca tggtcacttc gtagttcttg ccgtcgaatc ttttcttgat
      181 ggatatcgat gggaggaaca cgaaccgcgc cttattctca agcgcataaa ggaaactcat
      241 caacgcatcc atgtcgatgc tacggatacc tacttcgatc atctccttct ggaactcgcc
      301 ttttttctcc gccttgagtt ccttgatggt agatatttcc acgccgtagg tctcgcgaac
      361 accgttgaga taactgttga gattggtctc gttgttctgt atcgccagca gggtcgccgc
      421 ctcgcgatcc ttcgcgcgcc ggaaggcctc ccggtgcacg gtcaccttct ccatgagcga
      481 ccgtcgttcc gctatctcct cgcgacgcgt atcgacggcg tcatcgaaga aaaggaacgt
      541 ggcggcgacc gcaaagagga taagcgcggt accgaagacg accagtatgt tcttttcccg
      601 cgaactgagc gtggcgaaag atgcgatcag ccgttctttc ataattcttc ctcccacggc
      661 accgtttcct tcccggccgg ttcaggcccg cgggccggtg aacgggacgg ttccgcccgc
      721 tccgccggcg gcggcacgtt cttcggcgca tcaccatccg cttgttctgt cgggcttact
      781 ttttcctttt ccttttcctt tcccttgtcc ttctctttct ttgcgtgttc tttagccgac
      841 cgcttctgga aggtgaagcc gatgttgaac gaatttttcc ctccgcgact ggtgatctgc
      901 cccttgttca attcggtcgc gtactccatc ttttccaatt gctccagcag ggtgttgata
      961 tggtcgagcg attccgcttc gccggtcaat ttgaccttgc catcctttat cgagagatcc
     1021 ctcagttcta tggtgcttcc atcgaatgag gcgagaggga acacctgttc catgatgaac
     1081 agcgaggaat agggatacag gcgcttgtcg cctttcgcgt ccttcccgtc cttcccgtcc
     1141 ttgccgccac ctcctatcgt cttctgcatg accgaaagcg cctgccggag cgaggggtat
     1201 tcctttccca gtatctcctt ggtcacggtc cgcgcctgtt cgtccagcgc gtcaagggcc
     1261 cggtcgagat accaaaggcg cagttcgaac gccgttatca tgagcacgaa tgccagcgca
     1321 aaaagcgcgg cggcgagcag gagtcgtttt ttgaggaagt tgaacccgcc gcgatagacg
     1381 aactcgccac gcgagaaact ggggctctcg ccctgttcgg cgcgcaggaa tatccgcgcc
     1441 aaggcatcct cggcggagat caccatcgcg tcggggatca cagaggcgat cagtttctcg
     1501 gcgttgcccg gcaggcggtc ggcaaagcag acgaccgtac gccggtcgcg ccgcatgaat
     1561 ctgccgagat cggccaccat ctcctgaaag aactgttccg tcgccttgcg catgagcagt
     1621 tcgtcctcgg aaagcgctgg accgtccgac agatccatcg aggcggcgag cgtctgcaac
     1681 acctcctctt ccgttttggc gccgccgagc gacgagcgca tcatcgctac gagtcgcgaa
     1741 agacccgtgg tgcgcacgac ctgcccccgt tcgccgaaca gaacgatttg atcgtcatcg
     1801 atgaacagcc actgcatacc cgccccttcg ccgatcacca gcgccgtttc gggaataaga
     1861 ctccgcaccc ggtcccggag gggcgcgccg ctccggtcga tgatggcgtg cagatccttt
     1921 ttctgcgcca cgaagaccgg cacgatgccg tcggagcgaa gcggcgcaag gtcgaacacc
     1981 acctcgcttt ccttgaacgg ggtggtcgct tcgatatcct gcgcgacgat cctctcgagc
     2041 gccggacggg gaaccgtcgg gtaggcgctg gcaaggtaaa gcgtcgcggc ggcgggcacc
     2101 acgatgtcaa tatggtcgta ctgcgcaagt gccgccggcg cctcttcgaa agagcatact
     2161 gtaaagcgtt gcgcggccaa cctgttgtat tccctttcga attcgagcat gaccacccgg
     2221 tcctgctgta tcctgcacac ggcgcatttc atagcgtctc tccgttatcc ttctcgatag
     2281 aagtatatgt tgaagttctt gtcgctcacc acctcgatgt atgattcgat gccgtcgcgg
     2341 atgccacgca cccggatacg gtagaaactg ccttcggcgc tcaactggct tgcgaccgcg
     2401 ggctccagct cgaccccctc ctcgcggacc gcagtgataa aatcatccac tttgtagaat
     2461 ccgtcacggg cccgacgcgc cagcaccttg cccatgaatt cccgcatgat atcctcgttg
     2521 tagaacatcg actggttctt gtctttcgcg taacttcgga tgatcgattc cagaatgatg
     2581 gggtgcgcct tgttgacatt gatgcgtccg ctcgaataga tcgtaacgaa cggtgcgagg
     2641 agacggaacg ccacctcgtc cacgccgtac accatccgca gttcgtcgac ggtatcgaag
     2701 cgggcgttct tcacctcgta gcgcggcgag aactcctcgt acatcgaccg ctcgtcgccg
     2761 ccgatcaggt agcgctcgtc atcgccatcc acccagtcgg cgatgttcgc aatgagctcc
     2821 tcgcgggtga cgaactcacg acgtgaggtg ttctcgagga acaggaaatc gtacacctgt
     2881 ggctcaacaa gcccccagag catgcgggcg accgtctcct tggcggcgct gtcaagcaga
     2941 ttgatgttta tcttggtgtc ttcgttctcg aattcaagtt tgaaatcgcc gggaaaatcg
     3001 aacagcgggt cactctcgtc gaccacgggg gcaaccgtcc cctcctcctt cccggtcacc
     3061 gccgttccct cttccgcact caatatcgaa tcggtgtcgg caaaggtgtc ggcaccgctg
     3121 aaggcccgca ggatgctggt gtcgaacggc acgatgtccc agatctcgaa actctgcagg
     3181 ccgagttcct tgagcccgga ggacttcagc aggccttcta tttgtttctg caggtcgaac
     3241 actatggcgg cgaaggtgag ccccgattgc gcgagcacct gcgattcggc ctttttcttg
     3301 tagttcatcg cgaggtcgaa ctcggtgcgc gcctcgtagt tcatgtcggt gacgatcggc
     3361 agcatcacgg cggtcatcac cgacatgaac tacgagg
//
LOCUS       DAOQUE010000316         3374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_32241_length_3374_cov_4.065381, whole genome shotgun sequence.
ACCESSION   DAOQUE010000316 DAOQUE010000000
VERSION     DAOQUE010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32241_length_3374_cov_4.065381"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PKH10_09495"
     CDS             complement(<1..903)
                     /locus_tag="PKH10_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28398.1"
                     /translation="MSTAFDPMGAIQETTAGRRLFIGRAAPGPQQQILMAHYDISRMG
                     AAIFLFAFAAQMEERQETLTDIAQRYRITGAPYRTLLSEYFTLVKKKMIVSIPYQKNA
                     SPLCCAVEPLLMKMLVQGDLGGDPFDPADPFCMIQAIADLWEQRDHNEITPSYFSDEA
                     KRLLKKMSPENRLHELIVPFPFAEQIMFIIAAIDHIAAHRSGVDLREFAGNFFKVLRD
                     RANFLRDVAQEKIEIIREGLILLNDRGSDPFSRSPEFTLSPTARMRLFGGKNTARREK
                     VKIPAIFSHRRWRDKGPDLYFDDRL"
     gene            1032..1979
                     /locus_tag="PKH10_09500"
     CDS             1032..1979
                     /locus_tag="PKH10_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNT28399.1"
                     /translation="MSQARRTFAIHELLKSRKIVPLRTLAEELEVSDRQVKRDIAAMR
                     RDMKAPIEYSRERNGYYYTKPYALCDYGDEKMLLFYLFAQRIAKNLSLMPVVARDLLD
                     TIEDKYLKEYQPILDRISYELADHERFRPGIVKRVLDAMRSGTALGVVYQNAAGERSQ
                     RFIEPWHLMNYSGKWYILAWCRSSKEPRTFLLSRIIESMETGEPFEKELDERLLKKYL
                     EESYGIFKLPEAQEMTIRFHEPILPIVRDREWHPKQRMIEATCAGKPCLELTLPVADP
                     IEITYMVLGFAPHAEVVAPKEYRKYWLGRLKEAVKRFPR"
     gene            2065..>3374
                     /locus_tag="PKH10_09505"
     CDS             2065..>3374
                     /locus_tag="PKH10_09505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT28400.1"
                     /translation="MLGPIFAAVGLLIITGALFFLRATLRTLPFNFWVVNIMELAERL
                     AFFGSRAVLPLYMVASADQHGLGISYAEKGIIFGIWAAFQCLLPIVSGGFTDNFGYKK
                     SLYIAFTLNTVGYASMAFATGFWTMLGAAVMVGTGTAIFKPPVQGTVARSVTPENSSI
                     GFGMFYWVVNIGAMLAPIMAASLRGNENAPTWHIVFFACAAITALNFLPAFFLYREPE
                     TTGAGKSSLTVFKETGLNLLHDRKMLFFLLISSGFWLMFMQVWDLLPNFIDEWVDSRD
                     IAPIFHAILGDSALTAAGHVKPELIVNLDATAIVLFVMLISWFMSRFKMIVSLILGMA
                     FATVGFFGSGFFMTGLPVALMILVFSLGEIICSPKFSEYVGVNAPPDKKALYMGYSNM
                     PFAFGWMAGNFLSGPLYDLLASRTVFARRYLAEQLMVPDGDIAA"
BASE COUNT          631 a         1091 c          999 g          653 t
ORIGIN      
        1 gagccggtca tcaaaataga ggtcgggacc cttgtcgcgc cagcggcgat gcgagaagat
       61 ggcggggatt ttcacctttt cccggcgcgc ggtattcttc ccgccgaaga ggcgcatccg
      121 cgccgtgggt gacagggtga attcgggcga ccgcgagaag ggatcgctac cccggtcgtt
      181 gagcaggatg agcccttccc gtatgatctc gatcttctcc tgcgccacat cgcgcaggaa
      241 gttcgcccta tccctgagca ccttgaagaa gttcccggcg aactcgcgca ggtccactcc
      301 gctgcgatgg gcggcgatat ggtctatcgc cgcgatgatg aacatgatct gttcggcgaa
      361 ggggaagggc acgatcaact cgtgcaggcg gttctcggga ctcatctttt tgagcagtct
      421 cttcgcctcg tcggagaaat agctcggcgt tatctcattg tgatcgcgct gttcccagag
      481 atcggctatc gcctgtatca tacaaaaagg atcggcggga tcgaacgggt cgccgcccag
      541 atcgccctgc accagcatct tcatcagtag cggctccacc gcgcagcaga gcggcgaggc
      601 gttcttctgg taggggatcg aaacgatcat cttcttcttc accagcgtga aatattcgct
      661 cagaagcgtc cggtacggcg cccccgtgat gcggtaacgc tgggcgatgt cggtgagggt
      721 ctcctgccgc tcctccatct gcgccgcgaa ggcgaacagg aaaatggccg cgcccatgcg
      781 ggagatgtcg tagtgcgcca tcaggatctg ttgctggggt ccgggggcgg cgcggccgat
      841 gaacaggcgt ctgccggcgg tcgtttcttg aatagccccc atcgggtcga aagcggtcga
      901 catgtgttcc tccgtagttg ttacacacaa cgctccaccc taccacaccc ggtgggacat
      961 ggcgtgtccc cgaacttgac aaacgggacg cgcaacgcga tactgaagca ttctttcgag
     1021 ggaggatgac catgtcgcag gcacgccgca ccttcgccat ccacgaactg ctcaagagcc
     1081 gcaagatcgt cccgctccgg acgctcgccg aggaactgga ggtgagcgac cggcaggtga
     1141 aacgcgacat cgccgcgatg cgccgcgaca tgaaggcgcc catcgaatat tcgcgcgaac
     1201 ggaacgggta ttactacacc aaaccctacg ccctgtgcga ctacggcgac gagaagatgc
     1261 tcctgttcta cctcttcgcc cagcggatcg ccaagaacct gtcgctcatg ccggtggtgg
     1321 cgcgcgacct gctcgacacc atcgaggaca aatacctgaa ggaataccag ccgatactcg
     1381 accgcatcag ttacgaactg gccgaccacg agcggttccg ccccggcatc gtcaaacggg
     1441 tgctcgacgc gatgcgatcc ggtacggcgc tcggcgtcgt ttaccagaac gcggcgggcg
     1501 agcggtcgca gcggttcatc gagccgtggc acctgatgaa ctactcgggg aaatggtaca
     1561 tcctcgcgtg gtgccgctcg tcgaaggagc cgcgcacctt cctgctctcc cgcatcatcg
     1621 agagcatgga gaccggggaa ccgttcgaga aggaactcga cgaacggctg ctcaagaagt
     1681 atctcgagga gagttacggc atcttcaaac tgcccgaggc gcaggagatg accatccggt
     1741 tccacgaacc gatactgccc atcgtccgcg accgcgaatg gcacccgaaa cagcggatga
     1801 tcgaggcgac ctgcgccggg aagccgtgtc tcgaactcac cctgccggtc gccgatccca
     1861 tcgagatcac ctacatggtg ctcgggttcg ccccgcacgc cgaggtggtc gccccgaagg
     1921 aataccggaa gtactggctg gggcggctaa aagaggcggt gaagcggttc ccccgctgaa
     1981 acgccgaaga taccgccaca atcttgacaa accgccctcc cgaaaggtaa aagaggcggt
     2041 gccgtcaacc gccacggagt tcccatgctc ggaccgatct tcgccgccgt cggacttctc
     2101 atcatcaccg gagcgctctt tttcctccgc gccaccctgc ggacgctccc cttcaacttc
     2161 tgggtggtca acatcatgga actggccgaa cggctcgcct ttttcgggtc gcgcgccgtc
     2221 ctgccgctct acatggtcgc ctcggccgac cagcacgggc tgggcatctc gtacgccgag
     2281 aaagggatca tattcggcat ctgggcggcg ttccagtgcc tgctccccat cgtgagcggc
     2341 gggttcaccg acaatttcgg ctacaagaag agtctgtaca tcgccttcac gctcaacacc
     2401 gtcgggtacg cctcgatggc cttcgcgacc ggcttctgga cgatgctcgg cgccgcggtc
     2461 atggtcggca ccggcaccgc gatattcaag ccgccggtgc aggggaccgt cgcccgctcg
     2521 gtgacgccgg agaattcgag catcggcttc gggatgttct actgggtggt caacatcggc
     2581 gcgatgctcg cccccatcat ggcggcaagc ctgcgcggca acgagaacgc ccccacctgg
     2641 cacatcgtct tcttcgcctg cgccgccatc accgcgctca acttcctccc cgccttcttc
     2701 ctgtaccgcg aaccggagac gaccggcgcc gggaaatcgt cgctgaccgt gttcaaggag
     2761 accgggctca acctgctcca cgaccgcaag atgctgttct tcctactcat ttcgtccggt
     2821 ttctggctca tgttcatgca ggtgtgggac ctcctgccca atttcatcga cgagtgggtc
     2881 gattcgcgcg acatcgcgcc gatcttccac gcgatactgg gcgacagcgc gctgaccgcc
     2941 gcgggccatg tgaagcccga actcatcgtc aatctcgacg cgaccgccat cgtgctgttc
     3001 gtcatgctca tatcgtggtt catgtcgcgg ttcaagatga tcgtatcgct catactcggg
     3061 atggccttcg cgaccgtcgg tttcttcggg tccggcttct tcatgaccgg cctgccggtc
     3121 gcgctcatga tactcgtatt ctcgctcggc gagatcatct gctcgccgaa gttctcggaa
     3181 tatgtcgggg tgaacgcgcc gcccgacaag aaggcgctct acatgggcta ctccaacatg
     3241 cccttcgcct tcggctggat ggcgggcaat ttcctgtcgg ggccgctgta cgatctgctc
     3301 gcctcgcgga ccgtcttcgc gcgacggtat ctcgccgaac agctgatggt ccccgatggc
     3361 gatatcgcgg ccat
//
LOCUS       DAOQUE010000317         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_32309_length_3368_cov_4.380018, whole genome shotgun sequence.
ACCESSION   DAOQUE010000317 DAOQUE010000000
VERSION     DAOQUE010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32309_length_3368_cov_4.380018"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PKH10_09510"
     CDS             <1..844
                     /locus_tag="PKH10_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28401.1"
                     /translation="EVFDSTFEENAPVVIEVRDATLTVAGSFFTGNEGPSISTSGGLA
                     TITGTIFENNAAVNGGAIENSGSDMAIVRCRFVGNHADLPGSGFAGIGGAIFATMAGT
                     MTVADTVFDGNSAALWGGAVALIMSSPIFLNCTFDGNSDSTGDRAIFMAQSDPTFDNT
                     ILWGLVGQHVGELGPFDFEESVPLFRYSDVKDSGGSTSDCGIGGDEYCWVLPFGTDGG
                     GNIDEDPLFVGSGDDPLDLQATSPCVNTGNNDLVPDEEYALYDLLAADRIQSVTVDMG
                     AYEQ"
     gene            972..2684
                     /locus_tag="PKH10_09515"
     CDS             972..2684
                     /locus_tag="PKH10_09515"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HNT28402.1"
                     /translation="MCRGAIHRALVLLSLFLIACGGGNTATADADQADQTDQADQSDQ
                     SDQSDQSDQSDQTVDDDLIDLPDTDEPDIPPTILSLSVVMNPNEAAPLVGVITATTEP
                     ATRLRVTLESEEGTETLRYAALATQHRLPILGLLPGVTYTVTVSVETTSGAQIAYPDT
                     FTLVAPPLPDNFPTIQHVFSRPVAEREPGYLFIVLSMAQGIAEPFGFQVIAFDIAGKV
                     RWLRRDADFQHGMTTLPNGDLLFQFNQKIIEVNMFGDIVGEWRPEGVVGGPLAVDLPG
                     VQRFHHSVMEATDGAMVALDTEFRMMDDMPTSDTDPDGPRDTVMVLSDAIVEFDRDGA
                     IRSRRLLLDMLDPGRLGYLFIPGYQWSHTNYALPIPEDDSMLISVRHQSCVVKYDRTT
                     GVIKWILGNHDNWSAEFQQYLLTPVGTPFEWQWYQHNAKLTPDGTILMFDNGNFRAMP
                     YDTPIPDEDNWSRAVEYRVNETDMTVEQVWEWRLEGEDRIFSDSLGDVHYLPQTGNVI
                     VNFGHIAIGTTVKARIFEVTHANPAEVLYELRIDDASVNDTGWMVHSSEFRLSLYPDG
                     MLEE"
     gene            2694..>3368
                     /locus_tag="PKH10_09520"
     CDS             2694..>3368
                     /locus_tag="PKH10_09520"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNT28403.1"
                     /translation="MQRILVTGGAGFIGSHLCKRLLEEGAEVLCLDNFSTGSRRNIRP
                     LLSHPNFELITHDITVSISLEVDRIYHLASPASPVRYQHQPVMTVRTNVQGSLTALDL
                     ARATGATILQASTSEIYGDPEVHPQTEDYWGRVNPIGPRSCYDEGKRCAETLFFDYRR
                     QYGIPIKVARIFNTYGPHMAADDGRVVSNFIVQALAGDDITLYGDGSQTRSFCFVADM
                     VEGLVKL"
BASE COUNT          676 a         1030 c         1007 g          655 t
ORIGIN      
        1 cgaggtgttc gacagcacct tcgaagagaa tgcgccggtg gtcatagagg tcagggacgc
       61 gacgctgacg gtagccggat ctttcttcac cgggaacgag gggccatcca tatccaccag
      121 cggcggcttg gcgacgataa cgggcaccat cttcgagaac aacgcggcgg tgaacggcgg
      181 cgccatagag aactccggga gcgatatggc catcgtgcga tgccggttcg tcggcaacca
      241 cgccgatctt ccgggaagcg gctttgcggg catcggcggc gccatattcg ctacgatggc
      301 gggcaccatg accgttgccg acaccgtttt cgatggtaac agcgcggcgc tgtggggcgg
      361 cgcggtggcg ctcatcatgt cgtcgccgat cttcctcaac tgtaccttcg acggcaacag
      421 cgattcgacc ggtgaccggg cgatattcat ggcgcagagc gacccgacct tcgacaacac
      481 gatactctgg ggactcgtcg gccagcatgt cggcgaactc ggccccttcg atttcgagga
      541 gagcgtcccg ctgttccggt acagcgatgt aaaagacagc ggcggttcca ccagcgactg
      601 cggcatcggc ggcgacgaat actgttgggt gcttccgttc ggcaccgacg gcggcggcaa
      661 catcgacgaa gatccactct tcgtcggttc gggcgatgac ccgctcgacc tgcaggcgac
      721 atcgccgtgc gtgaataccg gcaacaacga tctcgtgccg gatgaggaat atgccctgta
      781 cgaccttctc gccgccgacc gcatacagag cgtcacggtc gatatggggg cgtatgagca
      841 gtaaaagaat acctcatccc tcccttctcc ttgaaaaagg agaaggagta aaaggtaggg
      901 gcgagattca tcacgccctc cttgccctct cctcttgcaa ggagagggcg gcaaagccgg
      961 gtgaggtatg tgtgtgtcgg ggcgcgatcc atcgcgccct cgttctttta tccctgttcc
     1021 tcatcgcctg cggtggcggc aacacggcca cggctgatgc cgatcaggcc gatcagaccg
     1081 atcaggccga tcagtcggac cagtcggacc agtcggacca gtcggatcag tcggatcaga
     1141 ccgtcgacga cgatcttatt gatctacccg acaccgacga gcccgacatc ccgccgacca
     1201 tcctctccct ttcggtcgtg atgaacccga acgaggcggc gccgcttgtc ggcgtcatca
     1261 ccgccacgac cgaaccggcg acccgcctgc gggtgacgct cgaaagcgag gaggggacgg
     1321 aaaccctgcg ttatgcggcg cttgctaccc agcaccgcct gccgatactc ggtcttttgc
     1381 cgggggtgac ctacaccgtt accgtgtcgg tcgaaacgac atccggtgcc cagatcgcat
     1441 accccgatac ttttacattg gtcgcgccac cgcttcccga caattttccg acgatacagc
     1501 atgtcttctc tcgtcccgtc gcggaacggg agccggggta tcttttcatc gtgctcagca
     1561 tggcgcaggg gatcgccgaa ccgttcggtt tccaggtgat agccttcgat atcgccggca
     1621 aggtccgctg gctgaggcgc gacgctgatt tccagcacgg gatgacgacg cttccgaacg
     1681 gcgatctcct ttttcagttc aaccagaaga tcatagaagt gaacatgttc ggcgacatcg
     1741 tcggtgaatg gcgacccgag ggggtggtcg gtggtccgct tgcggtcgat ctgccgggtg
     1801 ttcagcgctt tcaccattcg gtgatggaag cgaccgatgg cgccatggtc gcgctggaca
     1861 ccgagttccg catgatggac gatatgccga cgagcgatac cgaccccgac gggccgagag
     1921 atacggtgat ggtgctaagc gacgccatcg tcgagttcga tcgcgacggc gccatccggt
     1981 caaggcgttt attgcttgat atgctcgatc ccggacgctt gggctatctt ttcattcccg
     2041 gttaccagtg gagccacacg aattacgccc tgccgatacc ggaggacgat agcatgctta
     2101 tctcggtgcg gcaccagtcg tgcgtcgtca aatatgaccg gacgactggc gtgatcaagt
     2161 ggatactcgg caatcatgac aactggtccg cggaattcca gcagtacctt ctgacgccgg
     2221 tcggaactcc gttcgaatgg caatggtacc aacataacgc caaattgacg ccggatggca
     2281 ccatcctcat gttcgacaac ggcaatttca gggctatgcc gtacgatacg ccgatacctg
     2341 atgaggataa ctggagtcgc gcggtcgagt atcgggtgaa cgagaccgat atgacggtcg
     2401 aacaggtgtg ggaatggcgt ctggaaggag aggatcgcat cttttccgac tcgctcggcg
     2461 atgtccacta tttgccccag accggcaatg tcatcgtgaa tttcggtcac attgctatcg
     2521 gcacaacagt aaaagcccgc atcttcgagg tgacccacgc gaatcccgcc gaagtgcttt
     2581 atgaactacg gatagacgat gcatcggtga atgataccgg ctggatggtc cacagttctg
     2641 aattccgcct ctcgctgtac cccgacggga tgctggaaga ataggagata cccatgcaac
     2701 ggatactcgt gaccggcggt gccggcttca tcggttccca cctctgtaag cgcctgctcg
     2761 aagagggggc cgaggtcctg tgtctcgaca acttctcgac cgggagccgc cgcaacatac
     2821 gcccgcttct ctcgcatccg aacttcgaac tcatcaccca cgacatcacc gtgtcgatat
     2881 cgctcgaagt ggaccggata taccacctcg cctcgcccgc atccccggtc cgttaccagc
     2941 atcagccggt catgacggtg cggaccaatg tgcaggggtc gctgacggcg ctcgacctcg
     3001 cgcgcgcgac cggcgcgacc atcctgcagg catcgacgag cgagatatac ggtgacccgg
     3061 aggtccaccc gcagaccgag gattactggg gtcgcgtcaa ccccatcggc ccgcgcagtt
     3121 gttacgacga gggcaaacgg tgcgccgaga cgctcttttt cgactaccgc cggcagtacg
     3181 gtatccccat caaggtcgcc cgcatattca acacctacgg gccgcacatg gcggccgatg
     3241 atgggcgcgt ggtcagcaac ttcatcgtgc aggcgctcgc cggcgacgac atcacgctct
     3301 acggcgacgg ctcgcagaca cggtcgttct gcttcgtggc cgatatggtc gaggggctcg
     3361 tgaaactc
//
LOCUS       DAOQUE010000318         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_32311_length_3368_cov_3.509206, whole genome shotgun sequence.
ACCESSION   DAOQUE010000318 DAOQUE010000000
VERSION     DAOQUE010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32311_length_3368_cov_3.509206"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKH10_09525"
     CDS             complement(<1..562)
                     /locus_tag="PKH10_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28404.1"
                     /translation="MKRQPCARITCEVYQEISRTVLSALGSIGIRRPYIQSGRSIMLG
                     ERRGGLFSTGTRLLEDPIDIYSFTVPAERELAVMHRLIQAAMLDQEGRGSIYSEQIER
                     VSRDDECPGDESPQATAGSPHYPMIQHLVGISCIVQRGQSDIIVRSALEMGICVPSVT
                     FGIGAGLRDKLGLIRITIPAEKEVVNL"
     gene            complement(559..2694)
                     /locus_tag="PKH10_09530"
     CDS             complement(559..2694)
                     /locus_tag="PKH10_09530"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HNT28405.1"
                     /translation="MTGNKTPRQPKDRVRISFQQAMGLIIPYVKSRIMEQIRSVWLIL
                     LYLILFQTLVLQIPIVDGAVIAFGMVMVIFGLAFFMEGLLLGLMPLGELIGLKLPQKA
                     GLAVILIFSLLLGFGVTLAEPAIGALKILGGTVKAWDAPLLFTLLNTYPNYLVWAVGA
                     GVGLAVVFGMLRFLYNWSLKPFLYVLVPILTAVSVWAAFDPNLLYLTGLAWDCGAVTT
                     GPVTVPLVLALGIGITRVVGRGDSGASGFGVVTLASLFPILAVLLLGVSFSGKVPQPM
                     PGAEFMSPAQRATALGLFDGSEDDLLRYAFAQGGGDEVRAYFGEDANRRAFVADMARD
                     GSARTRLFGADEPRFMRWVAVHGSEAEKMALFGSIKSAREYTLNPVSPPPSSLTLLFL
                     RNFQAAAQAIIPLTLFMLFVFFVILREKLPHTDEIIFGIVIALLGMGFFNIGMETGLS
                     KLGDQVGGKVPSSFTTVPLEEQRASINGFSPDIVASAVDADKGGVARFFFHRHDRVVD
                     PIPYDEKNYDEMTGTYTYVPQVGPLFGGVWGVLVALLFAFIMGYGATLAEPALNALGI
                     KVEELTVGTFKKSFLMQAVAIGVGAGIAFGVAKIIWDIPLIWLLAPPYAILLVVTYLS
                     SEEYVNIAWDSAGVTTGPVTVPLVLAMGLGIGTRIGVVEGFGILAMASVYPILSVLLT
                     GLYAAYMRKRSGTGQEEEEASVGDGGATP"
     gene            complement(2769..>3368)
                     /locus_tag="PKH10_09535"
     CDS             complement(2769..>3368)
                     /locus_tag="PKH10_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28406.1"
                     /translation="GDTYAHLRMVGSIEHPVAYSPAEGLAEHDLRWDGAPFDATSYNN
                     FTTRTDNGLGEEVLFAQSFGDLRALNANTYTFNAAELIVPVAALEAAKLNEEVSLTAA
                     DAFFFSIHRYTQKIRDGEISYKKCFVAARDAEDTGASILPCYADNDEFAIGEPVKLFG
                     AMNLVADPASLGFYWPTTDDCLCFTAAGAPLDCAEFDGL"
BASE COUNT          659 a         1065 c         1076 g          568 t
ORIGIN      
        1 cgaggttgac cacctccttc tcggccggta tggttatgcg aatgagcccg agtttatcgc
       61 gcaggccggc gccgatgccg aaggtgacgc tcggcacgca gatgcccatc tcgagcgcgc
      121 tccggacgat gatgtcgctc tgtccgcgct gcacgataca ggagatgccg accagatgct
      181 gtatcatggg gtaatgcggc gatcctgcgg tggcctgcgg cgactcgtcg ccggggcact
      241 cgtcgtcacg cgacacccgc tcgatctgtt cgctgtatat cgaccctcgc ccctcctgat
      301 cgagcatcgc cgcctgtatg aggcggtgca tcacggcgag ttcccgttcc gccggcacgg
      361 taaaggaata aatgtctatc gggtcctcca gcagtctcgt gcctgtcgaa aagaggccac
      421 ccctcctctc gccgagcatg atggatcggc ccgactggat gtagggacgc cggatcccga
      481 tcgagccgag cgccgacaga acggtcctcg atatctcctg atagacctcg caggttatcc
      541 gggcgcaggg ctgccgtttc atggggtggc acccccgtct cccacggacg cttcttcctc
      601 ttcctgcccc gtaccggatc gtttgcgcat ataggccgcg tataacccgg tgaggagcac
      661 cgaaagtatc gggtagaccg acgccatggc aagtatgccg aacccttcga ccacaccgat
      721 gcgcgtcccg atgccgagtc ccatcgcgag cacgagcggc acggtgaccg gcccggtggt
      781 gacccccgcg ctatcccacg cgatgttgac atattcctcg ctcgacaggt aggtgacgac
      841 gagcaggatg gcatagggcg gcgcgagtag ccagatgagc gggatgtccc agatgatctt
      901 ggcgaccccg aaagcgatgc cggcgccgac gccgatggcg accgcctgca tcaggaacga
      961 tttcttgaag gtaccgaccg tcaactcctc gaccttgatc ccgagcgcgt tcagcgccgg
     1021 ctcggcgagc gtcgcgccat agcccatgat gaatgcgaac agcaaggcga ccaatacacc
     1081 ccagacacca ccgaacaacg gtccgacctg cggaacatag gtgtaggtgc cggtcatctc
     1141 gtcatagttc ttctcgtcat acggtatcgg atcgaccacg cggtcgtgcc ggtggaaaaa
     1201 aaagcgggcg accccgccct tatcggcgtc caccgcgctc gctacgatat cgggcgagaa
     1261 tccgttgatg ctggcgcgtt gttcctccag agggaccgtg gtgaaggagg acggcacctt
     1321 cccgccgacc tgatcgccca gtttggaaag ccccgtttcc atgccgatgt tgaagaagcc
     1381 catgccgagg agtgcgatga cgatgccgaa gattatctcg tcggtgtgcg gcagtttctc
     1441 gcgcaggatg acgaagaaca cgaacagcat gaacagcgtg agcgggatga tcgcctgcgc
     1501 ggccgcttga aagttacgca ggaagagcag tgtgagcgac gacggcggcg gcgacacggg
     1561 gttcagcgtg tactcgcggg ccgattttat cgacccgaaa agcgccatct tctcggcctc
     1621 gctcccgtgg accgccaccc accgcatgaa tcgcggctca tcggcgccga acaggcgggt
     1681 gcgggcgctc ccgtcgcggg ccatgtcggc gacgaaagcg cggcggttgg catcctcgcc
     1741 gaaataggcg cggacctcgt cgccgccgcc ctgcgcgaag gcatagcgga gcaggtcgtc
     1801 ctcactgccg tcgaacaggc cgagcgctgt ggcgcgctgg gcggggctca tgaactcggc
     1861 ccccggcatc ggctgaggca ccttccccga gaacgacacg ccgagcagga gcaccgcgag
     1921 tatcgggaag agcgacgcga gcgtcacgac gccgaacccc gacgcgcccg aatcgcccct
     1981 gccgaccacg cgggtgatgc cgatgccgag tgcgagtacg agcggcacgg tgaccggccc
     2041 ggtggtgacc gcgccgcagt cccacgcgag cccggtgaga tacagcaggt tcggatcgaa
     2101 cgcggcccag accgatacgg ccgtcagtat cgggacgagc acatagagga acggtttcag
     2161 cgaccagttg tacaggaagc gcagcatacc gaacaccacg gcgagcccga cgccggcgcc
     2221 gacggcccac accagatagt tcgggtaggt gttcaaaagc gtgaacagga gtggcgcgtc
     2281 ccatgccttc acggtgccgc cgagtatctt gagcgcgccg atggccggtt cggcgagcgt
     2341 cacgccgaaa ccgagcagca gtgagaatat caggatgacg gcgagccccg ccttctgcgg
     2401 cagtttaagc ccgatgagtt ctccgagcgg catgagcccg agcagaagtc cctccatgaa
     2461 gaaggcaagc ccgaagatga ccatgaccat cccgaaggcg atgacggcgc cgtccacgat
     2521 gggtatctgg agcacgagcg tctggaagag tatgaggtaa aggagaatga gccacaccga
     2581 acggatctgc tccatgatgc ggctcttgac atacgggatg atgagcccca tcgcctgctg
     2641 gaacgatatc ctgacgcgat ctttcggctg tcgaggggtc ttgttcccgg tcacctgtcc
     2701 gtctcccacg cgatacggga cgaccctagc atgtcacgcg ggacaagtca aaaagtttcg
     2761 ggaacggttc acaacccgtc gaactccgcg cagtcgagcg gagcacccgc ggcggtgaag
     2821 cagaggcagt cgtcggtcgt cggccagtag aaacccagcg aggcggggtc ggccacaagg
     2881 ttcatggcac cgaacagttt caccggttcc cctatcgcga actcgtcgtt atcggcgtag
     2941 cacggcagga tggaggcgcc ggtatcttcc gcatcgcgcg ccgcgacgaa gcattttttg
     3001 taggatatct cgccgtcccg tatcttctgc gtatagcggt ggatgctgaa gaagaaggcg
     3061 tcggcggcgg tcagcgagac ctcctcgttc aatttcgccg cctcgagcgc cgccaccggc
     3121 acgatgagtt cggccgcgtt gaaggtatag gtgttggcgt tcagcgcccg aaggtcgccg
     3181 aaactctgcg cgaagagcac ctcctccccc agaccgttgt cggtccgcgt ggtgaagttg
     3241 ttgtaggatg tcgcgtcgaa cggcgcgccg tcccagcgca gatcgtgttc cgccagcccc
     3301 tccgcgggcg agtaggccac gggatgctcg atggaaccga ccatccgcag gtgggcgtag
     3361 gtgtcgcc
//
LOCUS       DAOQUE010000319         3365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_32345_length_3365_cov_3.895770, whole genome shotgun sequence.
ACCESSION   DAOQUE010000319 DAOQUE010000000
VERSION     DAOQUE010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32345_length_3365_cov_3.895770"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1627
                     /locus_tag="PKH10_09540"
     CDS             176..1627
                     /locus_tag="PKH10_09540"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HNT28407.1"
                     /translation="MRVLIASSEIYPFTRAGALSETVGALAKALAGRCEVAMVMPLYA
                     IIDSERFGIERTPHRFPISVSDHEEPAEIWQGTLPGSEVPVYFVASHYFERDALYGTA
                     TGDYPDNSERFVFFSRAVVEVAENIVRPDLIHCNDWQTALVPVYLKEAYAPYGRLATA
                     RTVLTIHNIAYQGKFWMYDLHILNLGWGVFSPDKLEFYNHINYLKGGIVYADAITTVS
                     HRYAEETRSAEFGHGLDGLMRQMAHKMTPIRNGIDADHWDPENDIHLPVRYATPDDEG
                     RSACRTALLAEFGLDRATDLPLFGMVTHINIDKGVDLLVRMLGEAAARGDALFAILGD
                     GEKLLTDALRNLAERFSDRIVLKTAYDERLAHLIHAGSDFYLMPSRHEPCGLNQMYAM
                     RYGTVPVVRAVGGLDETVIDMDEHPAHGTGLKFRDYTPEALQRRVADAVSLFRDDRDR
                     YRLMARRCMTADLGWREPAGRYLAVYERVLVEK"
     gene            1699..2532
                     /locus_tag="PKH10_09545"
     CDS             1699..2532
                     /locus_tag="PKH10_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNT28408.1"
                     /translation="MARMMSLLTVLAFLGGILTAQEADKSDAVQDVPSGKINWTKQYV
                     YATGSGAPDLKAPNVAVARLGAERVAKADAYRNLLEAIKGVTVVGTTTMKNSMEESME
                     VKTSVEGLVRGAEIVTTKYYSDGGVDVVIRVPLSSLYDKAAATGAADKELASKESVKA
                     GAAAPAADAKKTLVFDARGLKFAPTMFPAVYTDDGKIVYSKKQVKDEALKANGVARYV
                     KNDLDAVAALYGDASTLLVVKASKLKNKSDLVVGAAEVTAIQTKLAPDALSEGRVVIL
                     Y"
     gene            2547..>3365
                     /locus_tag="PKH10_09550"
     CDS             2547..>3365
                     /locus_tag="PKH10_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28409.1"
                     /translation="MKRSFVMVFCAVALMFGAVEASEKKVTVEGFGAVVNGNVELAKK
                     EATEDALRRALEMVIGATVSAETVVENYQLVSDKIISKVKGYIKAYKVLEAKEAQGVV
                     TAKVEVTVKESDLKNDLELLQTTLERMNYPKVVVMMAEQNVGATVFSFWWGGATAQTM
                     QSDIGAAEQALTAALLEKNFRIVDRNALATGAKVKPAFQVVDISNQDVLQLTKNIDAD
                     IVIVGKAMARTLGSVAGSTMTSNQANVTMRAINLKDGKVLGSSAGQAAQAHIDPV"
BASE COUNT          663 a          980 c         1129 g          593 t
ORIGIN      
        1 cgtcacggtg attcttgaga aaggtcttcc atgactgcgt ctgatcggcg gtcgggccgg
       61 tcttcttccg tatcgcccgc agagcgcgag cgaccccttc aaaaagttga ctcgggggcc
      121 gtttgttgct atactgtcgc ttggtctttt ttcttattta agaggaacgg cggctgtgag
      181 ggtccttatc gcttcttccg agatttaccc gttcacccgg gccggtgcgc tttccgagac
      241 ggtcggcgcg ctcgccaagg cgttggcggg ccggtgcgag gtcgcgatgg tcatgccgct
      301 ctacgccatc atagacagcg aacggttcgg catcgagcgg acgccgcacc gtttcccgat
      361 atcggtgtcg gaccacgagg agccggccga gatatggcag gggaccctgc cggggagcga
      421 ggtgccggtc tatttcgtcg cgagccacta tttcgagcgc gacgccctct acggcaccgc
      481 gaccggcgat taccccgaca actcggagcg gttcgtcttt ttttcacggg cggtcgtcga
      541 ggtggccgag aacatcgtgc ggcccgacct catccattgc aacgattggc agacggcgct
      601 cgtgccggtc tatctaaagg aggcctatgc gccctacggt cgtttggcga cggcgcggac
      661 ggtgctcacc atccacaaca tcgcctatca gggcaagttc tggatgtacg atctgcacat
      721 cctgaacctc gggtgggggg tgttcagccc cgataaattg gaattctata accatatcaa
      781 ctacttgaag ggcggcattg tctatgccga cgccatcacg acggtcagtc accgctacgc
      841 cgaggagaca cggagcgccg agttcggtca cggactggac gggctcatgc ggcagatggc
      901 ccacaagatg acgccgatcc gcaacggtat cgacgctgac cactgggacc ccgagaacga
      961 catacacctg ccggtccgct atgccacgcc cgatgacgaa ggacgatcgg catgccgcac
     1021 ggcgttgctc gccgagttcg ggctcgaccg cgcgaccgac ctgccgctgt tcgggatggt
     1081 cacccacatc aacatcgaca agggggtgga tcttcttgtg cggatgctcg gcgaggcggc
     1141 ggcacgcggc gacgcgctct tcgcgatact gggcgacggg gagaaactac tgaccgacgc
     1201 cctgcgcaat ctcgccgaac ggttctccga ccgtatcgtg ctgaagaccg cctacgacga
     1261 gcggctggcg cacctcatcc acgccgggag cgatttttac ctcatgccgt cgcgccacga
     1321 gccgtgcggg ctcaaccaga tgtacgcgat gcgctacggg acggtgccgg tcgtccgggc
     1381 ggtcggcggg ctcgacgaga cggtcatcga catggacgag catccggccc acgggacggg
     1441 actcaagttc cgcgactaca cgccggaggc gctccagcgg cgcgtcgccg acgccgtttc
     1501 cctgttccgc gacgatcgcg accgctatcg cctcatggcc cggcggtgca tgacggccga
     1561 cctcggctgg cgtgagccgg ccggccggta cctcgcggtc tatgaacggg tgctggtgga
     1621 aaaataatga agtgtttttc gaataataga gtgatcgaca ggtctatgat gatcgcggat
     1681 ttttttaagg agaacgccat ggcgcggatg atgtctcttt tgacggtgct ggctttcctc
     1741 gggggtatct tgacggcaca ggaggccgac aagagcgatg cggtgcagga tgtgccgagc
     1801 gggaagataa actggaccaa gcagtatgtc tacgcgaccg gtagcggcgc gcccgacctg
     1861 aaggcgccca atgtggcggt cgcgcggctc ggggccgaac gggtggccaa ggccgacgca
     1921 taccgcaacc tgctcgaggc gatcaagggg gtgacggtcg tcgggaccac gaccatgaag
     1981 aactcgatgg aggaatccat ggaggtcaag acctcggtcg agggactcgt caggggggcc
     2041 gagatcgtca cgacgaaata ttactccgat ggcggggtgg atgtcgtgat acgggtcccg
     2101 ctctcctcgc tgtacgataa ggcggccgcg accggcgccg ccgacaagga actggcgtcg
     2161 aaggaatcgg tgaaggcggg cgcggcggca ccggcggccg acgccaagaa gacgctcgtg
     2221 ttcgatgcgc gcggtctcaa attcgcgccg acgatgttcc cggcggtcta caccgacgac
     2281 ggcaagatcg tgtacagcaa gaagcaggtc aaggatgagg cgctcaaggc gaacggggtg
     2341 gcgcgctatg tcaagaacga tctcgatgcg gtggcggcgt tgtacggcga cgcctcgacg
     2401 ctgctcgtgg tgaaggcgtc gaaactcaag aataagtccg acctggtcgt cggcgccgcc
     2461 gaagtgacgg cgatacaaac gaaactcgcc cccgacgcgc tgagcgaggg tcgggtggtc
     2521 attctgtact gacgggaggg cgtgtcatga aaagatcctt tgttatggtc ttctgcgcgg
     2581 tggcgctgat gttcggcgcg gtcgaggcgt ccgagaagaa ggtgacggtc gaagggttcg
     2641 gcgccgtggt gaacggcaat gtcgaactgg ccaagaagga ggcgaccgag gatgccctgc
     2701 ggcgcgcgct cgagatggtc atcggtgcga ccgtttcggc cgagacggtc gtcgagaact
     2761 accaactggt gtcggacaag ataatctcga aagtgaaggg ctacatcaag gcctataagg
     2821 tactcgaagc gaaggaggcg cagggggtcg tgacggcgaa ggtcgaggtg accgtgaagg
     2881 aaagcgacct caagaacgac ctcgaactcc tccagacgac gctcgaacgg atgaactatc
     2941 ccaaagtggt cgtcatgatg gctgagcaga atgtcggcgc gacggtgttc tcgttctggt
     3001 ggggcggcgc gaccgcgcag acgatgcaga gcgacatcgg cgcggccgaa caggcgctca
     3061 ccgcggccct gctcgaaaag aacttccgta tcgtcgatcg caacgcgctt gcgaccggcg
     3121 ccaaagtgaa gccggccttt caggtggtcg acatcagcaa tcaggatgtc ctgcaactca
     3181 ccaagaacat cgacgccgac atcgtgatag tcggcaaggc gatggcccgc acgctcgggt
     3241 cggtcgccgg ctcgacgatg acctcgaatc aggcaaatgt cacgatgcgc gccatcaacc
     3301 tcaaggacgg caaggtgctc ggctcgtcgg cggggcaggc ggcgcaggcc cacatcgacc
     3361 cggtg
//
LOCUS       DAOQUE010000320         3349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_32566_length_3349_cov_3.937158, whole genome shotgun sequence.
ACCESSION   DAOQUE010000320 DAOQUE010000000
VERSION     DAOQUE010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32566_length_3349_cov_3.937158"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /gene="rpsI"
                     /locus_tag="PKH10_09555"
     CDS             complement(<1..157)
                     /gene="rpsI"
                     /locus_tag="PKH10_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNT28410.1"
                     /translation="MEQWYATGKRKTAIARVFLRPGNGEVTVNGKDPKEYFYTDTNVG
                     KLFAPFKV"
     gene            complement(180..611)
                     /gene="rplM"
                     /locus_tag="PKH10_09560"
     CDS             complement(180..611)
                     /gene="rplM"
                     /locus_tag="PKH10_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNT28411.1"
                     /translation="MNITRSLRKEDAQHKWFEIDATGKTLGRLATRIATILRGKDKPL
                     FTPHVDCGDYVIVTNADKIVLTGAKWDDKFYYSYSGYQSGMTAHAAKEMVTRRPDFIV
                     MHAVRGMLPKNKLGRAVIKKLKVYAGTEHPHQAQSPVKIEL"
     gene            complement(716..2626)
                     /locus_tag="PKH10_09565"
     CDS             complement(716..2626)
                     /locus_tag="PKH10_09565"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HNT28412.1"
                     /translation="MTRSVLIVCALFLSLFAACDNGSNAPGDADAATDSASDGDAILP
                     DNDEVVDPLAALFGDPALNPSGQAPLTAEIPFTPDIPGALALRIECPALEGADPYEKE
                     FAFTVGATIPIPVMGLYADCSNLVTITVFDGEGSARGTREFTLTTAPAPEDLPTATAV
                     GTYAGDAFTFIVYYLTRASEEEPEENGGVETVPGVIPEFIGIMFDKKGHIRWYSDLTY
                     KKIAAIEIVDGYIYGSDWGTEAGVLWWHDFMGRTGGSLDIGALGFLWIHHDVIKLPNG
                     HLVLTADPITNDYVEEYLIEVDMTAGTVVKEWDLRPVFPDVADLYRDIPPTSTETYGV
                     TNDPIHLNGIAYDPVDGGLVVSSQRSGIAKIAADGTLRWLLAPHLTRYIDDANGDGVS
                     DSFMANYDPDNQMTWIGDFTGENYTDERMPCGGAPAEGTYPFDFSYGEFLLTPLDGAG
                     APITDEQVLRGFIDGDSFRWPFRPHAPLLKDDGTLLLFDNGLSRGFTIINQDTFSRAV
                     AYRITPDGDGYGGTVEQTAEYLLAHDPMWHRFSAAVSDVDELPDGSLLITSGALGTAF
                     YPPLIMTQYGDGPIGAYIAQVDAETGDEMHSLLVERVISEKYPNAPFSVYRAERVDPY
                     ANFALPEGLTAR"
     gene            2753..3211
                     /gene="smpB"
                     /locus_tag="PKH10_09570"
     CDS             2753..3211
                     /gene="smpB"
                     /locus_tag="PKH10_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNT28413.1"
                     /translation="MAERKTIARNKKAWHEYEILEKFEAGLALTGTEIKSIRDGNVAF
                     RDSYVEIKNGEAILTGFHIAPYICGNRWNHAEDRPRTLLLHKREILKWSQKVKERGFT
                     VIPLELYLDEHGRAKMQVALVRGKALHDKRDTLRKKEMKRELDRARSGER"
BASE COUNT          657 a          972 c         1089 g          631 t
ORIGIN      
        1 tcaccttgaa gggggcgaac agtttgccga cattcgtgtc ggtgtagaag tattccttcg
       61 ggtccttgcc gttcaccgtc acttcgccgt tgccgggacg caggaatacc cgcgctatcg
      121 ccgttttccg cttaccggtc gcgtaccatt gttccatcgt atatcgctcc tgacctaggt
      181 tacaactcta ttttcaccgg tgactgtgcc tgatgcggat gctccgtccc ggcgtacacc
      241 ttgagtttct tgatgacggc gcgccccagt ttgttcttcg ggagcatccc tctcaccgca
      301 tgcatgacga tgaaatcggg gcgacgggtg accatctcct tggcggcgtg ggccgtcatg
      361 ccgctctgat agccggaata actgtagtag aacttgtcat cccacttggc gccggtcagc
      421 actatcttgt cggcattggt gactatcaca tagtcgccgc agtcgacatg gggggtgaac
      481 aggggcttat ccttgccgcg caggatggtg gcgatacggg tagcgagccg accaagtgtc
      541 ttgcccgtgg cgtcgatctc gaaccacttg tgctgcgcgt cctctttcct gaggcttcga
      601 gtaatgttca tggcgcacct ttcaagccaa agttaccgaa aaaacggagg tgatgatacc
      661 ggcggggccc tcgttgtcaa gaaaaaaatc tgccgctacc acgaacaggc cgttcttacc
      721 gggccgtaag accttccggc agggcgaagt tcgcataggg atcgacccgt tcggcgcggt
      781 agacgctgaa gggggcgttc ggatatttct cggaaatgac ccgttcgacg agaagcgagt
      841 gcatctcgtc cccggtctcg gcgtcgacct gcgcgatata ggcaccgatc ggcccgtcgc
      901 cgtactgggt catgatgaga ggcgggtaga acgcggtgcc gagtgcgccc gatgtgatga
      961 gcagagagcc gtcgggtagt tcatccacat cgctcaccgc cgcgctgaac cggtgccaca
     1021 tcgggtcatg cgccagaagg tattcagctg tctgttcgac cgtcccgcca tagccgtccc
     1081 cgtcgggagt gatccggtag gcgaccgctc ggctgaaggt gtcctggttg atgatggtga
     1141 acccgcgcga aagcccgttg tcgaacagga gcagggtgcc gtcgtcctta aggagcgggg
     1201 cgtgcggccg gaagggccat cggaaggagt cgccgtcgat aaagccgcgc aggacctgtt
     1261 cgtcggtgat cggggccccc gcgccgtcaa gcggggtcag caggaactcg ccgtaagaga
     1321 aatcgaaggg ataggtccct tccgccggag cgccgccgca gggcatccgc tcgtcggtgt
     1381 agttctcgcc ggtgaaatcg cctatccagg tcatctgatt gtccgggtcg tagttggcca
     1441 tgaaactgtc actcaccccg tcgccgttcg cgtcgtcgat gtagcgggtc aggtgagggg
     1501 caaggagcca ccgcaaggta ccgtcggccg ctatcttcgc gataccggag cgctgggagg
     1561 agacgacgag cccgccatcg accgggtcgt aagcgatgcc attcagatgt atcgggtcgt
     1621 tggtgacgcc gtaggtctcg gtactggtgg gcggtatgtc gcggtaaagg tcggccacat
     1681 cggggaacac gggccgcagg tcccattcct tgaccacggt cccggccgtc atgtcgacct
     1741 cgatcaggta ctcttcgaca tagtcgttcg ttatcgggtc ggcggtcagg acaaggtgac
     1801 cgttcggcag tttgatgaca tcgtggtgga tccagaggaa cccgagcgcg ccgatgtcga
     1861 gcgatccgcc ggtccggccc atgaagtcgt gccaccagag cacccccgcc tcggtccccc
     1921 agtcgctccc gtagatgtag ccgtcgacga tctctatcgc ggcgatcttc ttgtaggtga
     1981 gatcggaata ccagcggata tgcccttttt tatcgaacat gatgcctatg aactcgggga
     2041 tgacgcccgg gaccgtctcg accccgccgt tctcctccgg ttcctcttcg gaggcccggg
     2101 tgaggtaata gacgatgaag gtgaaagcgt cgccggcgta ggtgccgacg gcggtggcgg
     2161 tcgggagatc ctccggggcc ggtgcggtgg tcagcgtgaa ctcgcgggtg ccgcgggccg
     2221 aaccctcgcc gtcaaatacg gttatcgtga cgaggttcga acagtcagca tagagaccca
     2281 tcaccgggat cggtatcgtt gcgccgacgg tgaaggcgaa ctccttctcg tagggatcgg
     2341 ccccttccag agcgggacac tcgatccgca gggcgagcgc ccccggaatg tcgggggtaa
     2401 agggtatctc ggcggtgagc ggcgcctgtc ccgaggggtt gagcgccggg tcaccgaaaa
     2461 gcgccgcgag ggggtcgacg acctcgtcgt tgtcagggag gatcgcatca ccatcgctcg
     2521 cggagtcggt cgccgcatcg gcgtcgccgg gggcgttgct gccgttatcg caggcggcga
     2581 acagcgagag gaacagggcg cagacgataa gaacggatcg tgtcatgacg gggcctcctg
     2641 aagtcgtacc gtcggggata gtagcacgct ccggtctgaa atcaatatag tgggggaaga
     2701 aacttcacga acggccgctt ccgtgtataa tcggtccggc acggaggaga cgatggccga
     2761 acggaagacc atagcgcgca ataaaaaggc gtggcacgaa tacgagatcc tcgagaagtt
     2821 cgaggcggga ttggcgctca ccggcaccga gataaaatcg atacgcgacg gcaatgtcgc
     2881 ctttcgcgat tcctatgtgg agataaagaa cggcgaggcg atcctgaccg ggttccatat
     2941 cgcgccctat atctgcggca accgctggaa tcacgccgag gaccggccgc ggaccctgct
     3001 cctgcacaaa cgcgagatac tcaaatggtc tcaaaaggtg aaagagcgcg gcttcaccgt
     3061 catcccgctc gaactgtacc tcgacgagca cggccgcgcc aagatgcagg tggcgctcgt
     3121 gcggggcaag gcgcttcacg acaagcgcga taccctgcgg aaaaaggaga tgaaacggga
     3181 actcgaccgg gcgcggagcg gcgaacgatg acgatgcccg aacggaacac ccgtcagcgc
     3241 ggcgccgacg ccgaggaatt cgtcgccaag cacctcgaaa aaaaaggatt cacgatagcc
     3301 gaccgcaact tcacggtgcg cggcggcgag atcgacatcg tggcgcgga
//
LOCUS       DAOQUE010000321         3347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_32591_length_3347_cov_4.107533, whole genome shotgun sequence.
ACCESSION   DAOQUE010000321 DAOQUE010000000
VERSION     DAOQUE010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32591_length_3347_cov_4.107533"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3347
                     /locus_tag="PKH10_09575"
     CDS             <1..>3347
                     /locus_tag="PKH10_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HNT28414.1"
                     /translation="GNTIAGDGCSAACVVEAGWNCSATYMPTRCSRCGNGAREWYEQC
                     DDGNTTAGDGCSATCTIESAAWHCNGATPTVCRNCGNGIMEGNEFCDPGIIVTCASRS
                     DLVPPQGGTVTGNIVCDGCTSLVTANNCFKTYTCSAGLPANACWYNCEAVAPFSTTYT
                     YNQTWNGSAWVNADNYLFGFNASPAANSCQFKCRPNYTWNAGTSTCDQNQQRVSCGAK
                     PRSDTYELWNDGESGANIGTYLQTWDGVSGTWQPVLSPHYDAAIGPCHFKCAAGAVWD
                     AGLGQCRICGDNIKEGIEACDTSQFGTSTCQNTVTPPEGSATGGSLSCNLSCGLITTG
                     CTKSWNCVNKPVGTVYYGGGDTYSYGQAWNGSAWVNPNNYLDGYVASPAVNTCQYTCA
                     GAGYVYDGLACSKCGDGVPTGMEPCDDGDLRNDNACKNNCTWNTCRDGYVLTGTEACD
                     DGNAVDTDYCRNNCSWNTCGDGALKATPVNMTHLLPFDGNANDASGKGNNGTLLNGAT
                     YVALGGIWGSHGLSLDGIDDRVSIPTTGMSNLQGTIDYWIKPNGWSGGALGAANRHGS
                     FQTIASWSNGARGQMMILDNFEGAFYFRIVDETAASVDVSFANNTYYTSGNWTRVTVT
                     WSRITSRMKVYMNGGFVAETVWNQPIATPFESTAYIGIGHGNPQLGVIDHFRILNRAL
                     SDAEITNSMWQSFDGTSAGVDFSHRANNGTLVNGPTVVAGKVGNALSFNGSNQYVTVP
                     DDASQMRLGTAMTVETWVKIAANPGDWVRIVGKSDTDASCSNRNYGLWIASTGQILFQ
                     IGGSTGIWGNVMSVRTVTNNVWRHVAGTYDGANMRVYIDGVLEGQLAYTQTPITSAGP
                     LTIGSAVGCHGYLNGQMDETRIYQRALTQAELQNSIEYCDDSNGTNTDACTSACVGST
                     CGDTIVKSTAPAEVCDSGFLYNGAYGYCKSDCSAMGPRCGDGILTFTTASMVLRLNMD
                     EGSGTTANDSAGTAQNGTITSPWWVAAGNPYSTKFNNALYFDGGARYVTMANHADLQL
                     GASKASFTVSAWVNFPAGAAGTRFPIITKGTGSTLNYALWLEPLIGTSPAGHVSFVFH
                     PDPTGCGAHSDIAVTDGAWHHVVGVYD"
BASE COUNT          674 a         1098 c         1019 g          556 t
ORIGIN      
        1 acggcaacac catcgccggc gacggctgtt cggcggcctg tgtcgtcgaa gcgggctgga
       61 actgttcggc gacctatatg ccgacgcgct gttcccggtg cggcaacggc gcacgggaat
      121 ggtacgaaca gtgcgacgac ggcaatacga ccgcaggcga cggctgttcg gcgacctgca
      181 ccatcgagag cgccgcgtgg cactgcaacg gcgcgacccc gaccgtctgc cgcaactgcg
      241 gcaacggcat catggaaggg aacgagttct gcgatcccgg catcatcgta acttgtgcgt
      301 cgaggagcga tctcgtgccg ccgcagggag gcaccgtcac cggcaatatc gtctgcgacg
      361 gatgcacctc gctggtgacc gcgaacaact gcttcaagac ctacacctgc tcggcgggcc
      421 tgccggcgaa cgcctgctgg tacaactgcg aggccgtggc gcctttctcc acgacctaca
      481 cctataacca gacctggaac ggttcggcgt gggtgaacgc cgacaactac ctgttcggct
      541 tcaacgcgag cccggcggcg aattcctgcc agttcaagtg ccggcccaat tacacatgga
      601 acgcgggcac ctcgacctgc gatcagaatc agcagagggt cagttgcggc gcgaaaccgc
      661 ggagcgacac ctacgaactc tggaacgacg gcgaaagcgg cgccaacatc gggacctatc
      721 tccagacatg ggatggcgtc agcggtactt ggcagccggt cctttcgccg cactatgacg
      781 ccgcgatcgg cccctgtcat ttcaaatgcg ccgcgggcgc cgtgtgggat gccgggctgg
      841 gccagtgccg gatatgcggc gacaacatca aggagggtat cgaggcgtgc gacacgagcc
      901 agttcggcac ctcgacctgc cagaacacgg tcacgcctcc ggaaggttcc gcgaccggcg
      961 gatcgctttc ctgcaacctt tcctgcggtc ttatcaccac cggttgcacc aagtcgtgga
     1021 actgcgtcaa caaaccggta ggaacggtgt actacggcgg cggcgataca tacagttatg
     1081 gtcaggcatg gaacggctcg gcgtgggtga acccgaacaa ttacttggac gggtatgtcg
     1141 ccagtccggc cgtgaacacc tgccagtaca cctgtgccgg tgccggctat gtgtacgacg
     1201 gacttgcctg ctccaaatgc ggcgacggtg tgccgaccgg catggagccg tgtgatgacg
     1261 gcgatctccg caacgacaac gcctgtaaga acaactgtac ttggaacacc tgccgcgacg
     1321 gctatgtcct caccggcacc gaggcctgcg atgacggcaa cgcggtcgac accgactact
     1381 gccgcaacaa ctgctcttgg aacacctgcg gcgacggcgc gctcaaagcg accccggtga
     1441 atatgaccca cctgctcccc ttcgacggga acgcgaacga cgcgtcgggc aaggggaaca
     1501 acggcacgct cctgaacggc gcgacctatg tggccctcgg cggcatatgg ggctcccacg
     1561 ggctgagtct cgacgggatc gacgaccgcg tcagcatccc gacgaccggc atgtcgaacc
     1621 tgcaggggac catcgactac tggataaagc cgaacggctg gagcggcggg gccctcggcg
     1681 cggcgaaccg acacggctcc ttccagacca tcgcttcgtg gtccaacggg gcgcgcgggc
     1741 agatgatgat actcgataac ttcgagggcg ccttctactt ccgcatcgtg gatgaaacgg
     1801 ccgcttcggt cgatgtctcg ttcgcgaaca acacctacta caccagcggc aactggaccc
     1861 gcgtcaccgt cacttggagc aggatcacct cgcgcatgaa ggtctatatg aacggcgggt
     1921 tcgtggctga gaccgtctgg aaccagccca tagcgacgcc cttcgagtcg accgcctaca
     1981 tcggtatcgg ccacggcaac ccgcaactgg gcgtcataga tcatttccgc atactcaatc
     2041 gcgccctcag cgacgccgag ataaccaaca gcatgtggca gtcgttcgac ggcacctccg
     2101 ccggcgtcga tttctcccac cgcgccaaca acggcacgct cgtcaatggc ccgaccgtcg
     2161 tcgccggcaa ggtgggcaac gccctgtcgt tcaacggttc gaaccagtat gtcacggtgc
     2221 ccgacgacgc gtcgcagatg cggctcggca ccgccatgac ggtcgagaca tgggtgaaga
     2281 tcgcggccaa cccgggcgac tgggtacgca tcgtcggcaa gagcgatacc gatgcgtcct
     2341 gctccaaccg caactacggt ctgtggatag ccagcaccgg ccagatactg ttccagatag
     2401 ggggctcgac cggcatctgg ggcaatgtca tgtcggtgag gaccgtgacc aataatgtgt
     2461 ggcgtcatgt ggccggtacc tacgacggtg ccaatatgcg ggtgtacatc gacggcgtcc
     2521 tcgaggggca actcgcctat acccagaccc cgataacctc ggcggggccg cttaccatcg
     2581 gttccgcggt cggttgccac ggctatctca acggccagat ggatgagacg aggatatatc
     2641 aacgcgccct cacgcaggcc gagttgcaga acagcatcga atactgcgac gacagtaacg
     2701 ggaccaacac cgacgcctgt accagcgcct gcgtcggcag cacctgcggc gacaccatcg
     2761 tcaagtccac cgcgccggcc gaggtctgcg acagcgggtt cctctacaac ggtgcctacg
     2821 gttactgcaa atccgattgt tccgccatgg gcccgcggtg cggcgacggt atcttgacct
     2881 ttaccaccgc cagcatggtc ctgcggctca acatggacga agggagcggt accaccgcca
     2941 acgattccgc gggaacggcg cagaacggca ccatcaccag cccgtggtgg gtcgcggcgg
     3001 gcaatcccta ctcgaccaaa ttcaacaacg ccctctactt cgacggcggc gcccgttatg
     3061 tcaccatggc gaaccatgca gacctgcaac taggcgcgtc aaaagcatcg ttcaccgtgt
     3121 cggcgtgggt caacttcccg gcgggagcgg cgggcacccg gttccccatc atcacgaagg
     3181 gtaccggtag cacgctgaac tacgctctct ggctcgagcc gctcatcggc accagtcccg
     3241 cgggacatgt ttcgttcgtg ttccatcccg acccgaccgg gtgcggcgcg cactccgaca
     3301 tcgccgtgac cgacggcgca tggcatcatg tcgtcggtgt ctatgac
//
LOCUS       DAOQUE010000322         3333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_32770_length_3333_cov_3.522575, whole genome shotgun sequence.
ACCESSION   DAOQUE010000322 DAOQUE010000000
VERSION     DAOQUE010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32770_length_3333_cov_3.522575"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKH10_09580"
     CDS             complement(<1..249)
                     /locus_tag="PKH10_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28415.1"
                     /translation="MKKIILLSLLALIVSACAVERRAITARLHTDKIFYAAYMEWQES
                     IFGISDYCSKIKRCNFMDNNGMVCMDEQEAERILNPQKY"
     gene            complement(265..555)
                     /locus_tag="PKH10_09585"
     CDS             complement(265..555)
                     /locus_tag="PKH10_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28416.1"
                     /translation="MKRYVILAIIVSALMTSCYTPHWLVSNDFKGTDKTFKEFFVPLG
                     TSMFDENAQVFNYNIRVCTIDDNANLTQCVDTMVIGNVVADSRYGGGTFYAY"
     gene            complement(568..846)
                     /locus_tag="PKH10_09590"
     CDS             complement(568..846)
                     /locus_tag="PKH10_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28417.1"
                     /translation="MKKIVVLLLCLSLLATTGCSVRRILMAAEDHPTRQLTNFETIDQ
                     YWLLGLYQVGAHYRFWTCVDGNESLDCKVLCDNKESSDLDCVGFGWGK"
     gene            complement(857..1090)
                     /locus_tag="PKH10_09595"
     CDS             complement(857..1090)
                     /locus_tag="PKH10_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28418.1"
                     /translation="MKRLLLVGLVAAALCAACTNPRVIKQMTYRDSDKTFKAITVQEQ
                     FCGLPLYAVVKCSMAEDGALSQCRQMAVNFDYE"
     gene            1289..1780
                     /locus_tag="PKH10_09600"
     CDS             1289..1780
                     /locus_tag="PKH10_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT28419.1"
                     /translation="MTKVNSGDTVKVHYIGKLKEGGVFDSSRERNEPFIFKTGEGMVI
                     PGFNDAVVGMMIGEIKKVTIPPAEGYGDRLDEFVLKVPKNRFPEGADLTAGTTFALND
                     GMGRSLPATIVEIAGDEVTLDANHPLAGKTLMFEIELLEIGCALPKHAHCHDNSCGGG
                     CCG"
     gene            complement(1777..2619)
                     /locus_tag="PKH10_09605"
     CDS             complement(1777..2619)
                     /locus_tag="PKH10_09605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT28420.1"
                     /translation="MSEAYALLDHGEGRKLESFGGVILDRPAPQATGPRRYPKAWGAA
                     HASFLKGSGWRRLRPFDEPWRAKIDGIVLELALHAQGQVGLFPEQGPNWRWLRDAVAS
                     ADRDLTMLNVFAYTGGATIAPLTVRVPHRVEVCHLDGSKGAVTIARRNTALNDLGDAP
                     VRWLTDDALAFMKREKRRGRRYDGIVLDPPAFGRGPDGGQWKLADSLPDLLALAADLL
                     SERPLLMLLSWHDPGLVSARAVAMMREALTGKSISSVRGLALVLPCRGHGMLAAGRSV
                     RVLF"
     gene            complement(2616..3152)
                     /locus_tag="PKH10_09610"
     CDS             complement(2616..3152)
                     /locus_tag="PKH10_09610"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HNT28421.1"
                     /translation="MAHSVPVDPVKYLIAALYRRDAFPPSVEGALQERWGAIDLRGEE
                     HPFDLTDYYREEMGEGLVRRLLSFERLLPPTELVAMKLACNDIEDANASGGKRAVNLD
                     CGYLDHHKVVLASCKGAGHKLYLDSGIWGDMTCRWQKGAYRPFEWSFPDFKDDRYRKE
                     LAAIRERYLCQLRQGRTI"
     gene            complement(3142..>3333)
                     /locus_tag="PKH10_09615"
     CDS             complement(3142..>3333)
                     /locus_tag="PKH10_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28422.1"
                     /translation="PVLTRVTAAAHCYLQGTELRMRDNDRTSRVRFDKELSGEFIARH
                     IGIDPAIVKRAFGVWGHGA"
BASE COUNT          667 a         1042 c          985 g          639 t
ORIGIN      
        1 gtacttctgc ggattgagga tgcgctcggc ttcctgctcg tccatgcaga ccatgccgtt
       61 gttgtccatg aagttgcagc gctttatctt cgagcagtaa tcggagatgc cgaagatgga
      121 ttcctgccac tccatgtagg cggcgtagaa tatcttgtcg gtgtggaggc gggcggtgat
      181 ggcgcgccgt tcgaccgcgc aggcgctcac gatgagcgca agcagactca gaagaattat
      241 ctttttcatg tgcttcctcc cttactagta cgcgtaaaag gtgccgccgc cgtatctgct
      301 gtcggcgacc acattgccga tgaccatcgt gtcgacgcac tgggtcagat tggcattgtc
      361 gtcgatcgtg cagacgcgga tgttgtaatt gaagacctga gcgttctcat cgaacatgct
      421 ggtccccaac ggcacgaaga attctttaaa ggtcttgtcg gtgcccttga aatcgttgct
      481 gaccagccag tgcggcgtat agcacgaggt catcaaggcg ctgacgatga tggcgagaat
      541 gacatatctt ttcatggcat cacctcctta cttgccccaa ccgaacccga cacagtcgag
      601 atcggaggat tccttgttgt cgcacaggac cttgcagtcg agcgattcgt tgccgtcgac
      661 gcaggtccag aagcggtagt gggcaccgac ctggtacagg cccaggagcc agtactgatc
      721 gatcgtctcg aagttcgtga gttgacgcgt ggggtgatcc tcggcggcca tgaggatgcg
      781 ccgcaccgaa cagccggtgg tcgccaacag cgaaaggcac agcaacaata ctacgatctt
      841 tttcatggcc tcctccctac tcgtaatcga agttgacggc catctggcga cactgggaaa
      901 gcgcgccgtc ctccgccatg gaacatttca ccacggcata cagcggcagg ccgcagaact
      961 gctcctgcac ggtgatagcc ttgaaggtct tgtcgctgtc acggtacgtc atctgcttga
     1021 tgacgcgcgg gttcgtgcac gcggcacaca gcgcggccgc gaccaacccg accaaaagaa
     1081 gtctcttcat cagggtacct cctcgtcaca cggtttattg aacgccggaa aagaacggaa
     1141 tgttcgctcc tatcacaaag agggggccaa gtcaattttt ttaccactcc ccgccgtgaa
     1201 cgaaaaaaat aaaattgttg caatccatac cgaccgtgga taggatgcct caccgtttgt
     1261 tttttcccta acttggcagg aggcagacat gacaaaggtc aacagcggcg atacggtgaa
     1321 ggttcactat atcggcaaat tgaaagaggg cggcgtgttc gattcgtcac gcgagcggaa
     1381 cgagcccttc atcttcaaaa cgggcgaagg gatggtcatc cccgggttca acgacgccgt
     1441 cgtcggcatg atgataggcg agataaagaa ggtgaccata ccgcccgccg aagggtacgg
     1501 cgaccgactc gacgagttcg tgctgaaggt ccccaagaac cgattccctg aaggcgccga
     1561 tctgacggcc ggcacgacct tcgccctcaa tgacggcatg ggccgctcgc tcccggcgac
     1621 gatcgtcgag atcgccggcg acgaggtgac gctcgacgcc aaccacccgc tcgccgggaa
     1681 gacgctgatg ttcgagatag aactgctcga gatcgggtgc gcgctcccca aacacgcgca
     1741 ctgtcatgac aactcttgcg gtggcggctg ctgcggctag aacaggaccc gcaccgaccg
     1801 gccggccgcc agcatcccat gcccccgaca cggcagaacc aatgcaagcc cccgcaccga
     1861 agagatgctt tttcccgtga gcgcctctcg catcatcgca acggcccggg ctgacacgag
     1921 cccgggatca tgccacgaca gaagcatgag taacggtcgc tcggacagaa gatccgccgc
     1981 aagagcaagc aggtcgggca ggctgtcggc gagtttccac tgcccgccgt cgggtccccg
     2041 cccgaacgcg ggcggatcga ggacgatacc gtcgtaacgc cgaccacgcc gtttttcccg
     2101 tttcatgaac gccagcgcgt cgtcggtcag ccagcggacc ggcgcatcgc cgaggtcatt
     2161 cagggcggtg ttgcgccgtg cgatcgtcac cgcccctttc gagccgtcga ggtgacagac
     2221 ctccacgcgg tgcgggacgc ggaccgtgag cggcgcgatc gtcgcgccgc cggtgtaggc
     2281 gaacacattg agcatcgtca ggtcgcggtc cgccgaagcg accgcgtcgc ggagccatcg
     2341 ccagttcggc ccctgctcgg ggaagaggcc gacctgtccc tgcgcgtgca gcgcgagttc
     2401 gagaacgatg ccgtctatct ttgcccgcca cggctcatcg aagggccgaa gtcgtcgcca
     2461 gccgctccct ttcaggaaag aggcgtgcgc cgctccccat gccttcggat accggcgcgg
     2521 accggtcgcc tgtggcgcag gcctatcgag gatgacgcct ccgaacgatt cgagtttcct
     2581 cccctcgccg tggtccagca gcgcataggc ttcgctcata tcgtcctacc ctgcctgagt
     2641 tggcaaagat accgttcccg gatcgcggcg agttccttcc ggtaccgatc atccttgaaa
     2701 tcgggaaaac tccattcgaa cggccggtag gcccccttct gccagcgaca ggtcatgtcc
     2761 ccccagatac cgctgtcgag gtaaagtttg tggcccgccc ccttgcacga ggcgagcacc
     2821 actttgtgat gatcgaggta gccgcagtca agattgaccg cccgtttgcc gcccgaagcg
     2881 ttcgcatcct cgatatcgtt gcaggccagt ttcatcgcca cgagttcggt cggcggcagg
     2941 agtcgttcga acgaaagcaa ccgtcggacg agtccctccc ccatttcctc ccggtaatag
     3001 tcggtcaggt cgaacggatg ttcctccccg cggaggtcga tcgcgcccca gcgttcctgc
     3061 agcgcccctt cgacggaagg cggaaaggcg tcgcgccggt agagcgccgc gatgagatat
     3121 ttgaccgggt cgaccgggac gctatgcgcc atggccccat accccgaagg cccgtttgac
     3181 gatcgcggga tctatgccga tatggcgggc gatgaattcg cccgatagtt ccttgtcgaa
     3241 ccggacacgg ctcgtccggt cgttatcccg catccgtaac tcggtgccct gcagatagca
     3301 gtgggccgcc gcggtcaccc gcgtcagcac cgg
//
LOCUS       DAOQUE010000323         3319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_32963_length_3319_cov_4.022978, whole genome shotgun sequence.
ACCESSION   DAOQUE010000323 DAOQUE010000000
VERSION     DAOQUE010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32963_length_3319_cov_4.022978"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..906
                     /locus_tag="PKH10_09620"
     CDS             7..906
                     /locus_tag="PKH10_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28423.1"
                     /translation="MPVTATSYPGLALMIESIGMSHMMELPMVIILAQRLGPATGTAT
                     GGATGDLSLLHGINSGGYPLPTLCISDPRDAWELSAKAVHIADRLRCPVILLASKETV
                     MTVQDVDPAVMTPIEPVKRRFYTGDAPYRPYEPEPDGVPPLLPVGDARHQVRITASTH
                     DRSAALQHSTPEALANSRRLAEKVEKNVGDYTLFDWDDDAADTVVVAYDVTAAAAREA
                     VALLRAEGRKASLFIPKTLFPVPPKYFEILGRYKKVVIAEENLQGQYREILFGKRGRD
                     GVRGVTAVGRLITPEEIAAEVRS"
     gene            903..2177
                     /locus_tag="PKH10_09625"
     CDS             903..2177
                     /locus_tag="PKH10_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT28424.1"
                     /translation="MNGRFLSTENLPFCKGCGHHIVSQNLEKALSSIEGFAPLDVIVV
                     TDIGCIGIIDKQFATHTVHGLHGRSVALAAGIAMATGNPKKKIIALLGDGGATIGLQH
                     LMEAAQRNINLTVIVHNNMLYGMTGGQPSGLTPRGFKTPIMPDGKPNIGYDLCRVLHA
                     AGAPSVRRLLALGDFSNDLAEALKVPGFSFVEAMEICPSYGVKFNPNRKLDDIAKEAG
                     IEVGHWAGPAREPYRPATHAAPASLFAAEKPIAARHKASLKERYAVVLSGSAGEGVQS
                     AAEFLAQAALSCGLHATKKGSYPVTVGVGFSTAEILLSPQPLHFTGISVPDAVIATSQ
                     DGLNKVRGLVEKMTKGTLYLDASLEAPATKATVVKHDFRGPLGGRSAVLVALTMLLDR
                     AGIMPTDAFLSVLKESSLGKKLDFAKLRQTAG"
     gene            2184..3203
                     /gene="tsaD"
                     /locus_tag="PKH10_09630"
     CDS             2184..3203
                     /gene="tsaD"
                     /locus_tag="PKH10_09630"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNT28425.1"
                     /translation="MTRYILGIESSCDDTSAAVVDEHFRVLAVRTVSQTNLHALFGGV
                     IPEIAARNHIAWIFPTIDKALSESGVAPTDLSAVAVTNYPGLLGSLLVGVGAAKGLAI
                     GWGKPLIAVNHLEAHIHSPFLGGRERPKPPYLALVVSGGHTTLMAVGEGSAASVLAET
                     MDDAAGEAYDKIAKIAKLSYPGGPAIDKIAQTGDPSRYDLPHLMRSGRYKDTLAFSFS
                     GIKSAVKRILEEEGDTVALADLMAGFQSRIVELIERRLTAAWEQKAYTALVVAGGVAA
                     NSAVRKMAQEFTGKRRVPLYLPELTYCQDNGAMVAAAAFPKLDRGDLASLDLDVTATS
                     RPQTK"
BASE COUNT          613 a         1128 c         1044 g          534 t
ORIGIN      
        1 ggataactgc cggttaccgc gaccagttat ccggggctcg ccctcatgat cgaatcgatc
       61 ggcatgtcgc acatgatgga actgccgatg gtcatcatac tcgcccagcg gctcggcccc
      121 gcgaccggca ccgcgaccgg cggtgcgacc ggcgacctgt cgctgctcca cggtatcaat
      181 tcgggcggct atccgctgcc gaccctctgc atctccgacc cgcgcgacgc gtgggaactc
      241 tcggccaagg cggtccatat cgccgatcgg ctccgctgcc cggtgatact gctcgcctcg
      301 aaggagacgg tcatgacggt gcaggatgtc gatccggcgg tcatgacgcc tatcgagccg
      361 gtgaagcgcc gcttctacac cggcgatgcc ccgtaccgcc cgtacgagcc ggagcctgac
      421 ggcgttccgc cgctcctgcc ggtgggcgac gcgcgccatc aggtgcgcat caccgcatcg
      481 acccatgacc gttccgccgc gctccagcac tcgacccccg aggcgctcgc caacagccga
      541 cggctcgccg agaaggtaga aaagaacgtc ggcgactaca cgctcttcga ctgggacgac
      601 gacgcggccg acacggtcgt ggtcgcttac gacgtgacgg cggcggcggc gcgcgaggcg
      661 gtcgccctgc tgcgcgccga gggacggaag gcgtcgctgt tcatccccaa gacgctcttc
      721 ccggtgccgc cgaagtactt tgagatactc ggtcgctaca aaaaggtcgt catcgccgaa
      781 gagaacctgc agggacagta ccgcgagatc ctgttcggga agaggggtcg cgacggcgtg
      841 cgcggcgtca ccgcggtcgg tcggctcatc actcccgaag agatagcggc ggaggtgcgg
      901 tcatgaacgg acgattcctt tccaccgaga atctgccctt ctgcaaaggg tgcggtcacc
      961 acatcgtgtc gcagaacctc gagaaggcgc tgtcgtctat agagggattt gcgccgctcg
     1021 atgtcattgt cgtgaccgac atcggctgca tcggcatcat cgacaagcag ttcgccaccc
     1081 atacggtcca cgggctccac ggccgttcgg tggcgctggc cgccggcatc gcgatggcga
     1141 ccgggaaccc gaagaagaag atcatcgcgc tcctcggcga cggcggcgcg accatcgggc
     1201 tccagcacct catggaggcg gcacagcgca acataaacct caccgtcatc gtccacaaca
     1261 acatgctcta cggcatgacc ggcggccagc cctcaggact caccccgcgc ggattcaaga
     1321 cccccatcat gcccgacggg aaaccgaaca tcggctacga cctgtgccgg gtcctccatg
     1381 cggcgggcgc cccgtcggtg cggcggctcc tcgcgctcgg cgacttctcc aacgatctgg
     1441 ccgaggcgct caaggttccc ggattctcct tcgtcgaggc gatggagatc tgtcccagtt
     1501 acggggtgaa gttcaacccc aaccgcaaac tcgacgacat cgccaaggag gcgggtatcg
     1561 aggtcggcca ctgggccggc ccggcgcgcg aaccgtaccg tcccgcgacc catgccgcgc
     1621 ccgcgtcgct tttcgcggcg gaaaaaccga tagcggcgcg ccacaaggca tcgttgaaag
     1681 agcgctatgc ggtggtcctt tccggctcgg cgggcgaagg ggtgcagtcg gcggccgaat
     1741 tcctcgcgca ggcggctctt tcctgcggtc tccacgccac caagaaggga agttacccgg
     1801 tcacggtcgg cgtcggcttc tcgaccgccg agatactgct ttccccccaa cctctgcact
     1861 tcaccggcat cagcgtcccc gacgcggtga tcgccacctc gcaggacggc ctgaacaagg
     1921 tgcgcggcct cgtcgagaag atgacgaagg ggacgctcta tctcgacgcc tcgctcgagg
     1981 cgcccgccac caaggcgacg gtggtgaagc acgatttccg cggcccgctc ggcggccgca
     2041 gcgcggtgct tgtcgcgctg accatgctcc ttgaccgcgc cggcatcatg ccgaccgatg
     2101 cctttctctc cgtgctcaag gaaagttctc tcggcaagaa actcgacttc gccaaactgc
     2161 ggcagacggc cggatagcgc cgcatgacgc gctacatcct cgggatagag agtagttgcg
     2221 acgatacctc ggcggcggtg gtggatgagc atttccgcgt gctggcggtc cggaccgtgt
     2281 cgcagacgaa cctccacgcc cttttcggcg gcgtcatccc cgagatcgcg gcgcgcaacc
     2341 acatcgcctg gatattccct acgatagata aggcgctttc cgaaagcggc gtcgccccga
     2401 ccgacctttc ggctgtcgca gtgaccaatt atcccggact gctcggatcg ctgctggtcg
     2461 gcgtaggcgc ggccaaaggg cttgcgatcg ggtggggaaa gccgctcatc gccgtcaacc
     2521 acctcgaagc acacatccac agcccgttcc tcggcgggcg cgaacgaccg aagccaccct
     2581 acctcgcgct ggtcgtatcg ggcggccata cgacgctcat ggcggtcgga gaggggagcg
     2641 cggcgagcgt gctcgccgag acgatggacg acgcggcggg cgaggcctat gacaagatcg
     2701 cgaagatcgc caaactgtcc tatccgggcg gtccggcgat agacaagata gcgcagacgg
     2761 gagatccttc ccgttacgac ctgccgcatc tcatgcggag cggaagatac aaagacacgc
     2821 tcgccttcag tttcagcggc atcaagtcgg cggtgaagcg catcctcgag gaagagggcg
     2881 atacggtggc gcttgccgac ctcatggccg gttttcagtc gcgcatcgtc gaactgatcg
     2941 aacggcggct gaccgcggcg tgggaacaaa aagcctacac ggcgctcgtg gtcgccgggg
     3001 gcgtcgccgc caacagcgcg gtacggaaga tggcgcagga attcaccggc aagcgtcgcg
     3061 tgccgctgta cctgccggaa ctcacctact gtcaggacaa cggcgcgatg gtcgcggcgg
     3121 cggcattccc gaaactcgac cgcggcgatc tcgcgtcgct cgacctcgat gtgacggcga
     3181 cgagccgtcc gcagacgaaa tgaacctgac catcctgaaa caactgctcg ccgacgccgg
     3241 tgtcgcaccg cgcaaaggtc tttcgcagaa cttcctcttc gacgacaccg cgctcgaaaa
     3301 gatcgccgac gcgctgacc
//
LOCUS       DAOQUE010000324         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_33310_length_3296_cov_3.275532, whole genome shotgun sequence.
ACCESSION   DAOQUE010000324 DAOQUE010000000
VERSION     DAOQUE010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33310_length_3296_cov_3.275532"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PKH10_09635"
     CDS             complement(<1..727)
                     /locus_tag="PKH10_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28426.1"
                     /translation="MADQRITGRDAPRTFLDTVDEGIKQKGYEFASSFFALWKSLKMY
                     GEQNEAVKKAVDKTLDVLHSFFKTQPTINFGYNGTDIVINDQRLKGKRGGEDYLDMLA
                     RLFTSLYVGEIEISSTVTQQDLVLFCQLTRNVSLDQPGTEDTFRRIEAEISGRIASMH
                     VSMYDPIEDELPPIIDKPQMARQVYRNLVTECPLFRKKALQKQPLPLKKATRSIQNLV
                     DLLTDDEEDRQWSHLLFLASLDSY"
     gene            complement(728..2635)
                     /locus_tag="PKH10_09640"
     CDS             complement(728..2635)
                     /locus_tag="PKH10_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT28427.1"
                     /translation="MGLKGHSRDVTDFFRDHSGEEAKYEHLRGQAEEIVLLFHKVLKG
                     YKLYPINNPMFQKFAEDFKTKVDLMHTEIPVIPFRIKKTGFLFMKQPVETALDDNEVI
                     YVLFNDGIRELYFSKGITYEEILAFFNILAKVTVYASEDYDIATLLWDNAFEHIGYIT
                     EEELVESEPMEYDDFDQGYDLKAEANRGRDLTPETFADRDVPMQDRETTLLSDEDRAF
                     FAQQMDLYNETAITNRFLKEMTTELLLGKNEEVRNDLVETAALLWERLLLFGAVKEAV
                     IFLKALLKIGGQFRTTNETFYQKIKSGIANIADEEFINRAFRIAENIDPDQFPYFGEF
                     LAVVPPQQLPFIVMKLSELQSSELRIAALEKIGPTIRNPEMLKPILAHDDWHIVRNGL
                     SLLKALNNPAVVPLLRGVINHPQKQIRVEAIAVLMAYSVEEALPALEKAVFSPEKDIR
                     LMSVEKLLELKNPQVKSVINRLLQESNLAKLDPAEVKSYLEKIISERRQDLYDLVAVL
                     LFSRIPSLRDTTLRLIGRVDSTRPIVRHILKFIEAPEFEKLPRAELDLFLPLIRAENL
                     SDTIRPLEKVLKLSGPLLKRDAFHDLKDAVISHLDRHRSDPAVKLFLKKGLACGNKET
                     EEIIRSKGGLL"
     gene            complement(2691..>3296)
                     /locus_tag="PKH10_09645"
     CDS             complement(2691..>3296)
                     /locus_tag="PKH10_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28428.1"
                     /translation="TTVTGRRDAVFFDDALLWRKGEHLRKFLSFVEPGLFRFHTPNGL
                     HLREVDEDIAHLLFSYRFSPLRFGFETGGDRFDSKKDGVRLADTLAILHRVGYRPRDL
                     GVYLLCGMPGQTLREVEDAIELVVAAGGRPYLNEYSPVPGTQLFDEHLTESARDISGE
                     PLWQNNSLSAYRSPVFTPSVMRRLKDHLASVYRDQDRERFS"
BASE COUNT          606 a          887 c         1059 g          744 t
ORIGIN      
        1 ggtaactgtc gagcgacgcg aggaacagga ggtgcgacca ctggcggtcc tcctcgtcgt
       61 cggtgagcag gtcgacgagg ttctggatgc tccgtgtcgc ctttttaagc gggagcggct
      121 gtttctggag cgcctttttg cggaacagcg ggcattcggt gacgagattc cggtacacct
      181 gtcgcgccat ctgcggtttg tctatgatgg ggggcagttc gtcctctatc ggatcataca
      241 tcgagacatg catcgaggcg atgcggccgg agatttcggc ttcgatgcgc cggaaggtat
      301 cctcggttcc cggctggtcg agcgagacat tgcgggtgag ttggcagaac aagacgaggt
      361 cctgctgggt gaccgtggag gatatctcta tctcgccgac atagagcgag gtgaagaggc
      421 gggcgagcat gtcgagatag tcctcgccgc cccgttttcc cttaagcctc tggtcgttga
      481 tgacgatgtc ggtgccgtta tagccgaaat ttatcgtggg ctgggtcttg aaaaatgaat
      541 gcaggacatc gagcgtcttg tcgaccgctt tcttgaccgc ctcgttctgt tcgccgtaca
      601 tcttgagcga tttccagagc gcgaagaagg atgacgcgaa ctcgtagcct ttctgcttta
      661 tcccctcgtc cacggtgtcg aggaaggtgc gcggtgcatc gcgcccggtg atgcgttggt
      721 cggccattta gagcagccct ccctttgaac gaatgatctc ctcggtctcc ttgttgccgc
      781 aggcgagtcc ttttttcagg aacagtttga ccgccgggtc ggaacggtga cgatcgaggt
      841 ggcttatcac cgcatccttg aggtcgtgga acgcgtcgcg tttgagcagg gggcccgaga
      901 gtttaagcac cttttcgagg ggccggatgg tgtccgaaag gttctcggcg cggatgagcg
      961 ggaggaaaag gtccagttcg gcgcgcggca gcttctcaaa ctcgggtgcc tcgatgaact
     1021 tgagtatgtg ccgcacgatg gggcgtgtcg aatccaccct gccgataagc cggagcgtgg
     1081 tgtcgcgaag cgacggtata cgcgagaaga gaagcactgc gacgaggtcg tagaggtcct
     1141 gccgtctttc cgagatgatc ttttccagat aacttttcac ctccgccggg tcgagtttgg
     1201 cgaggttcga ttcctgcagg agccggttga tgaccgattt cacctgcggg ttcttgagtt
     1261 cgagcaattt ctcgaccgac atgagccgga tatccttttc gggactgaac accgcctttt
     1321 cgagcgccgg gagcgcctcc tcgaccgagt aggccatgag gaccgctatc gcctcgaccc
     1381 gtatctgttt ctgcgggtga ttgatgacgc cgcgcaggag cgggacgacc gccggattgt
     1441 taagcgcctt gaggagcgac agtccgttgc gtacgatgtg ccagtcatcg tgggcgagta
     1501 tcggcttgag catctccgga ttgcgtatgg tcgggccgat cttctcgagc gcggcgatgc
     1561 gcagttcgga cgactgcagt tcgctcagtt tcatcacgat gaagggaagt tgttgaggcg
     1621 gcacgacggc gaggaactcg ccgaagtagg ggaactggtc cgggtcgatg ttctcggcga
     1681 tccggaaggc gcgattgatg aactcctcgt cggcgatgtt cgctatgccg ctctttatct
     1741 tctggtaaaa ggtctcattg gtggtgcgga actggccgcc tatcttgagg agcgccttga
     1801 ggaatatcac cgcctccttg accgcgccga acagaagcag gcgttcccag aggagcgcgg
     1861 cggtctcgac gaggtcattg cggacctcct cgttcttccc gaggaggagt tcggtggtca
     1921 tctccttgag gaagcggttg gtgatggcgg tctcgttgta cagatccatc tgctgggcga
     1981 aaaaggcacg gtcctcgtcc gacaggagcg tggtctcccg gtcctgcatc ggcacatcgc
     2041 ggtcggcgaa ggtttcgggg gtgaggtcgc gaccgcgatt cgcctcggct ttgaggtcgt
     2101 atccctgatc gaaatcgtca tattccatcg gttccgactc gacgagttct tcctcggtga
     2161 tgtagccgat gtgctcgaag gcgttgtccc acagaagcgt cgcgatgtcg tagtcctcgc
     2221 tcgcgtagac cgtcaccttg gcgaggatgt tgaagaaggc gagtatctcc tcataggtga
     2281 tgcccttcga gaaatagagt tcccgtatcc cgtcgttgaa gagcacatag atgacctcgt
     2341 tgtcgtcgag cgccgtttcg accggctgtt tcatgaacag gaacccggtc ttctttatgc
     2401 ggaacgggat gaccggtatc tcggtgtgca tgaggtccac cttggtcttg aaatcctcgg
     2461 cgaacttctg gaacatcggg ttgttgatgg ggtagagttt gtagcccttg agcaccttgt
     2521 ggaatagcag gacgatctcc tcggcctgtc cgcgcagatg ctcgtatttc gcctcctccc
     2581 cactgtggtc tcgaaagaaa tcggtgacat ccctgctgtg tcccttgagt cccatggctc
     2641 acaccctctg atgaacggtt cgaggcatta tagtgcctga ccgccggttt tcaagaaaat
     2701 cgctcgcgat cctgatcgcg gtacaccgag gcgaggtgat ccttgaggcg ccgcatcacg
     2761 gagggggtga acacggggga ccggtaggcc gaaagagaat tgttctgcca gagcggctcg
     2821 cccgatatgt cacgggcgct ttcagtcaga tgttcgtcga aaagctgggt cccggggacg
     2881 gggctgtatt cgttgagata cgggcgcccg cccgccgcca caacgagttc tatggcatcc
     2941 tcgacctcgc ggagggtctg ccccggcatt ccgcagagga gatatacgcc gaggtcgcgc
     3001 ggtcgatagc cgaccctgtg gaggatggcg agcgtgtcgg cgagccgtac cccgtccttt
     3061 ttcgaatcga accggtcacc gccggtctcg aagccgaacc ggagcggcga gaagcgatag
     3121 ctgaaaagga gatgcgcgat atcctcgtcc acctcccgca aatgcagacc gttgggggtg
     3181 tggaaccgga agaggcccgg ctcgacaaaa gagaggaatt tccggaggtg ttcacctttc
     3241 cgccacagca gggcatcgtc gaaaaagacc gcgtcgcgcc tgccggtcac cgtggt
//
LOCUS       DAOQUE010000325         3295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_33315_length_3295_cov_3.807099, whole genome shotgun sequence.
ACCESSION   DAOQUE010000325 DAOQUE010000000
VERSION     DAOQUE010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33315_length_3295_cov_3.807099"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2261)
                     /locus_tag="PKH10_09650"
     CDS             complement(<1..>2261)
                     /locus_tag="PKH10_09650"
                     /inference="COORDINATES: protein motif:HMM:NF025316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HNT28429.1"
                     /translation="SQTYTDANGWQPPTTAYYDATPDNGTCGYKCAANYWWNGSACVN
                     SRTSQSCTDLPSNAVYYNGTTSYSISQTYTDANGWQPPTTAYYGDGTVNINTCEYQCA
                     TSYHWGGSACVSNTRTWVCTPKPATGTVWNTVSQYAQTWTGGAWSPTDSATTYDLTGS
                     ESSCRYKCDGTNGYEWDPSILKCTKCGDGIPEGNEGCDDGNTVTEKCLYGLTIPNVTI
                     CSSSCGNAPCLVTYCGDDITDTDNDEECDDGNDKNTDACRNDCKDNICPDGFPHNGVE
                     ECDDGNEVNTDGCKNDCTDNVCGDGVINTGVEDCDNGSSNNGYNKPCTGDCKDNPCIY
                     ADGWYYYGGHCYKHFDTVAGFNAADGSCWTYSASLVSLSDSGEENFLHSAFPVPSAGD
                     SASWYWIGLTQEYNTQPGVITSPEYVWARPQPYAIYHGSTSGKANDHAAYPAPDMVFA
                     FAPTNAGNYHIKVYPKTASFNATLSIHDIYNGAWSHVNAAGDGAVEASGAYAMTAGRT
                     YYIVVDGLLADDAGGFALEILKDNVQPGWRWLDGAPYSYTNWFPGDPNNMDGWENAVL
                     KVTPQWQDAKTGGDATPRKYLCEKGGFTVCGDGYETGSEACDDHNTNDGDGCSSLCAV
                     EGGWTCLATVPTQCYKCGNGFKEAYEQCDDGNTNDTDGCKSDCTVDTANGWTCSATQP
                     SVCQKCGNGVPEWTEQCDDGNGDNDDACRNDCRDNICGDGHLNIGVEDCDDGNELNTD
                     ACKNDCTENYCGDG"
     assembly_gap    2262..2361
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2362..>3295)
                     /locus_tag="PKH10_09655"
     CDS             complement(<2362..>3295)
                     /locus_tag="PKH10_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28430.1"
                     /translation="LHTNICGDGYPCEDEECDDGNDDPCDGCLGGSCTFHTNFCGDGY
                     KCGIESCDDGNTVTEKCAYGLTVPNETVCSSSCGNVPCLTTYCGDGITDTNNDEECDD
                     HNTQNTDACKNDCRENFCGDDYLNIGVEVCEIDTTIACSAIPFGTGGTATCNGLCTEW
                     VTANNCTRTYVCATKPQTDTETAYNTVSNYMQTWNGSAWIPADDPTTEYNATTSATSC
                     QYTCAPHYTWNNTACAPDTKVWSCSGAPANTVYYSGTGSYSYSQVWGGSDWVPPESAA
                     GYSASPTQHTCQYKCATNYWWNGSACVNSRTSQS"
BASE COUNT          541 a          949 c          949 g          756 t
ORIGIN      
        1 ccgtcgccgc agtagttctc ggtgcagtcg ttcttacagg cgtcggtgtt caactcgttc
       61 ccgtcgtcgc aatcttcaac gccgatattc aggtgcccgt cgccgcagat gttgtcccgg
      121 cagtcgttcc ggcacgcgtc gtcgttgtcg ccgttcccgt cgtcgcactg ctcggtccac
      181 tcggggacgc cgttcccgca cttctggcag acgctcggct gggtcgcgga acaggtccac
      241 ccgttcgccg tgtccacggt gcagtcgctc ttacatccat cggtgtcgtt cgtgttaccg
      301 tcgtcacact gctcgtaggc ctctttgaag ccgttcccgc acttgtagca ctgcgtcggt
      361 acggtggcga ggcaggtcca gccgccctcg accgcgcata gggatgagca cccgtcgccg
      421 tcgttggtgt tgtggtcatc acaggcctcg ctgcccgtct cgtagccgtc gccacagacg
      481 gtgaacccgc ccttctcgca cagatatttg cgtggcgtgg catccccgcc cgtcttggcg
      541 tcctgccact gcggggtcac cttaaggacc gcgttctccc agccgtccat gttgttggga
      601 tcaccgggaa accagttcgt ataggaatag ggcgccccat cgagccagcg ccaaccgggc
      661 tggacgttgt ccttcagtat ctcgagggcg aaaccgcccg cgtcgtcggc aaggaggccg
      721 tccaccacga tgtagtaggt gcgtccagcc gtcatcgcgt aggcgccgct cgcctcgacc
      781 gcgccgtcgc ccgccgcgtt gacatggctc catgcaccgt tgtagatgtc atggatggag
      841 agcgtcgcgt taaaggaagc cgtcttggga tataccttga tgtggtagtt gccggcgttc
      901 gtcggcgcga acgcgaagac catgtcgggc gccggatagg cggcatggtc gttcgccttg
      961 ccgctggtgg acccatggta gatggcgtac ggctgcgggc gggcccagac atattccggg
     1021 gaggtgatca cgccgggctg ggtgttgtat tcctgcgtca ggccgatcca gtaccaagag
     1081 gcgctgtcgc cggcgctcgg gaccgggaag gcggagtgga ggaaattctc ctcaccactg
     1141 tcgctgagcg atacgagaga ggcgctgtag gtccagcagg atccatctgc cgcgttgaat
     1201 ccggcaacag tgtcgaagtg cttgtagcag tggccgccgt agtagtacca gccgtcggcg
     1261 tagatgcagg gattgtcctt gcagtccccg gtgcagggct tgttgtagcc gttattcgag
     1321 gagccgttgt cgcaatcctc aaccccggta ttgatcaccc cgtcgccgca gacattgtcg
     1381 gtgcaatcgt tcttgcagcc gtcggtgttc acttcgttac cgtcgtcgca ttcctcgacg
     1441 ccgttatggg gaaagccgtc cggacagata ttgtccttgc agtcgttccg gcaggcgtcg
     1501 gtgtttttgt cgttaccgtc gtcgcactcc tcgtcattgt cggtgtcggt gatatcatcg
     1561 ccacagtagg tcacgagaca cggggcattg ccgcatgaac tgctacagat ggtcacattc
     1621 ggtatcgtta ggccatagag acatttctcg gtcaccgtgt tcccgtcatc gcatccttcg
     1681 ttcccctccg ggataccgtc accgcacttg gtgcacttca ggatggaggg atcccattca
     1741 tagccattcg tcccgtcgca tttgtaccgg cacgagctct cacttcccgt cagatcgtag
     1801 gtcgtcgcgc tgtcggtcgg actccatgcg cccccggtcc aggtctgtgc gtactggctc
     1861 accgtattcc ataccgttcc ggtcgccggc ttcggcgtac agacccatgt ccgcgtgttc
     1921 gatacacagg cagagccacc ccaatgatag gaagtggcgc actggtactc gcaggtattg
     1981 atattcaccg tgccgtcgcc gtagtaagcg gtcgtcggcg gttgccagcc attcgcatcg
     2041 gtataggtct gcgatatcga ataggaggtc gtgccgttat aatataccgc gttggaagga
     2101 agatccgtgc aggattggct cgtccgcgag ttgacgcagg cagaaccgtt ccaccagtag
     2161 ttcgctgcac atttgtagcc gcaggtgccg ttatccggcg tagcgtcgta gtaagcggtc
     2221 gtcggcggtt gccagccatt cgcatcggta taggtctgcg annnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn ncaggattgg ctcgtccgcg agttgacgca ggcagaaccg
     2401 ttccaccagt agttcgtcgc acatttgtac tggcaggtgt gttgggtcgg actcgcgctg
     2461 taaccggccg cgctttcggg cgggacccag tcacttccgc cccatacctg ggaataggag
     2521 tacgatccgg tgccactgta gtacactgtg ttggccggag cacccgagca ggaccacacc
     2581 tttgtatcgg gggcacacgc ggtattgttc catgtgtagt gcggagcaca ggtgtactgg
     2641 caactcgttg cgctcgtcgt cgcgttatat tcggtcgtcg gatcgtcggc ggggatccat
     2701 gccgagccgt tccatgtctg catataattc gagacggtgt tgtatgccgt ttcggtgtcg
     2761 gtctgcggct ttgtcgcgca aacataggtt ctcgtgcagt tgttcgccgt cacccattcg
     2821 gtgcaaagac cgttacaggt cgccgttccg ccggtaccga acgggatggc gctacaggcg
     2881 atggtcgtgt cgatctcgca gacctcgaca ccgatgttca gataatcatc gccacaaaaa
     2941 ttttccctgc aatcattctt gcatgcgtcg gtgttctgcg tgttatgatc atcgcactct
     3001 tcatcgttgt tcgtatcggt gataccgtcg ccacaatagg ttgtcagaca ggggacattg
     3061 ccgcacgaac tgctgcagac cgtttcgttc gggacggtca ggccgtaagc gcatttctcg
     3121 gtgacggtgt tgccatcgtc acatgattcg atcccgcatt tatacccatc accgcagaag
     3181 ttggtatgaa atgtacatga tccgcctaaa cagccgtcac acgggtcatc atttccgtca
     3241 tcacattcct catcctcgca tggataaccg tcaccgcata tattggtgtg taagg
//
LOCUS       DAOQUE010000326         3293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_33350_length_3293_cov_3.720815, whole genome shotgun sequence.
ACCESSION   DAOQUE010000326 DAOQUE010000000
VERSION     DAOQUE010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33350_length_3293_cov_3.720815"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PKH10_09660"
     CDS             complement(<1..692)
                     /locus_tag="PKH10_09660"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HNT28431.1"
                     /translation="MRFNHYLVGALPKLTWDSELPSTLERFLDDHWFLLESLYDPLKD
                     ILLQNDLYNIELVLRSQIPRSKAGPDQGPQPIEIYKPNYLEVEDIVLFLQEPFQNMPD
                     PNFPEFIVDYFLQHRTPQERVDHLEDLFIGYFVYLQNCKSGFVRYYGRIATVIRTVLA
                     AQRILQLGLNLEEHLKGDPYIVQTILENRSSSDLGLKSIFPEVVDIINLFNKEPVELE
                     HDLDRIRFKLME"
     gene            complement(715..1350)
                     /locus_tag="PKH10_09665"
     CDS             complement(715..1350)
                     /locus_tag="PKH10_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28432.1"
                     /translation="MAGDLHEQTKNFEHKVKDISDYLRTNVLSPAEQERDRIIAEANA
                     EKDRIVAQAEKKAEQIVQTAEEKARRTIETAESSLRIAARQAIDTLKIALEKEVLSRT
                     VGGKARETLSDRAVMKELLGEMVRQTVASGMGQVSLLLSDDLRRDLGDALKKDIAAQA
                     TAGITLSDETVPSGFVVRFTDKGFAYEFTHEAVRDLLADLLRSDLREYLFK"
     gene            complement(1462..2703)
                     /locus_tag="PKH10_09670"
     CDS             complement(1462..2703)
                     /locus_tag="PKH10_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNT28433.1"
                     /translation="MVTVRPFKAFRPPRSLVEEIAALPYDVVNTAEAAAFAKGKPHSF
                     FHITRAEIDLPRDIDIHDDRVYTQGRDTLQRFIREGILVQDKEPLFYIYAQKMGDHVQ
                     TGLVGCVSADEYNADLIKKHELTRKDKEDDRTRHVVTQNANAEPVFLTYRTRKEIDEM
                     IFAHTGKNAPEYDLVADDGIRHTLWVIRDKALNDRIAAAFRTVPLLYVADGHHRSASA
                     ARAQAEKRKHDPNPSPDREYNYFLAVIFPHDQLRIMDYNRLVKDLNGNTTDQFLKKVG
                     ERFDISPATQAAPQGQTYFGMYLDGTWHQLKARPGTFDPKDPIGSLDVAILQTNLLAP
                     ILGIADPRTDKRIDFVGGIRGLGELEKRVDSGEMKVAFAMYPTTLDQLMAVADSGKIM
                     PPKSTWFEPKLRSGLFVHMLD"
     gene            2847..>3293
                     /locus_tag="PKH10_09675"
     CDS             2847..>3293
                     /locus_tag="PKH10_09675"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT28434.1"
                     /translation="MADTTSRIIIIGSSAAGLSAAQEARKTSPEATITLISEEPHLPY
                     HRPSLTEYIGDESVERRPQFLLQPAGWFAAQRIDLRRGIRAAGIDRQSRQVLLVSGEL
                     LPFDRLVLAVGAQPFVPMADALSRENVFAVRTLDDARRVAQCADRSH"
BASE COUNT          585 a          933 c         1051 g          724 t
ORIGIN      
        1 tcttccatca acttgaagcg gatgcggtcg agatcatgtt cgagttcaac cggctccttg
       61 ttgaacaggt tgatgatgtc gacgacctcg gggaagatgc ttttgagtcc gaggtcgctc
      121 gacgatcggt tctcgagtat cgtctgaacg atgtaggggt cgcccttcag gtgttcttcg
      181 aggttgagac cgagttgcag tatgcgctgg gcggcgagca ccgtccggat gaccgtcgcg
      241 atgcgaccgt agtaacggac gaacccactt ttgcagttct ggagatacac aaagtacccg
      301 atgaagagat cctcaaggtg gtcgacccgc tcctgggggg tgcggtgctg taggaagtag
      361 tcgacgatga attcggggaa gttcgggtcg ggcatgttct ggaacggctc ctgcaggaac
      421 agcacgatgt cctcgacctc gagatagttc ggcttgtata tctcgatggg ctggggcccc
      481 tgatcgggtc ccgctttcga ccgcggtatc tgcgaacgga gcaccagttc gatgttgtag
      541 aggtcattct ggagcaggat gtccttcaac ggatcgtaga gcgattcgag gaggaaccag
      601 tgatcgtcga ggaacctctc aagcgtggag ggcagttcgc tgtcccatgt cagtttcggc
      661 aacgccccga cgaggtaatg attgaatctc atccgtgttc acccggctcc gtcgtcactt
      721 gaacaggtac tcgcggaggt cggaacgcag aagatcggcg agcaggtcgc ggaccgcctc
      781 atgggtgaac tcgtaggcga accccttgtc ggtgaacctg accacaaaac cgctcggcac
      841 ggtctcgtct gaaagagtga tgccggcggt cgcctgtgcg gcgatatcct tttttaacgc
      901 atcgccgaga tcgcggcgca gatcatcgga aaggaggagg gagacctgtc ccatgcccga
      961 cgcaacggtc tggcggacca tctcgccgag cagttccttc atcactgcgc ggtcggagag
     1021 cgtttcgcga gcctttcccc cgacggtgcg ggagagcacc tctttctcaa gcgctatctt
     1081 cagcgtatcg atcgcctgtc gtgcggcgat acggagcgac gattcagccg tttcgatggt
     1141 ccgacgcgcc ttttcctcgg ccgtctgcac gatctgttcg gccttctttt cggcctgcgc
     1201 gacgatccga tccttctcgg cgttcgcctc ggcgatgatg cggtcccgct cctgttcggc
     1261 gggggacagc acattcgtcc gcaggtagtc gctgatatcc ttgaccttgt gctcgaagtt
     1321 cttggtctgt tcgtgaagat cgccggccat caccgttcct ccttgttgtc gcgcccgata
     1381 tgtaactgac cgaatagacg aggcactata tcctatcaat agcaaaagtc aacgataccc
     1441 ggagcggaga cgaagatgtt gtcagtccag catatggacg aagagacccg atcggagttt
     1501 cggctcgaac caggtcgatt tcggcggcat gatcttgccc gaatcggcga ccgccatcaa
     1561 ctggtcgagc gtcgtcgggt acatcgcgaa ggcgaccttc atctcacccg agtccacccg
     1621 tttctcgagt tctcccagtc cacggatgcc accgacgaaa tctatccgct tgtcggtgcg
     1681 cgggtcggcg atgccgagta tcggagccag gaggttggtc tgcagtatcg cgacatcgag
     1741 cgagccgata gggtccttcg ggtcaaaggt gcccggtctc gccttgagtt ggtgccacgt
     1801 tccgtcgaga tacatgccga agtaggtctg tccctgcggg gccgcctgcg tggcggggga
     1861 gatgtcgaat ctctcgccca ccttcttcag gaactgatcg gtggtgttgc cgttcagatc
     1921 cttcacgagc cggttgtagt ccatgatgcg caactggtcg tgcgggaata tgacggcaag
     1981 gaaataatta tattcacggt cgggcgacgg gttcgggtcg tgcttgcgtt tctcggcctg
     2041 tgcacgggct gccgaggcgc tccggtggtg cccgtcggcg acatagagaa gcggtaccgt
     2101 gcggaacgcg gcggctatgc ggtcgttgag cgctttgtcg cggatcaccc agagcgtatg
     2161 acggatgccg tcgtcggcga cgagatcgta ctcgggcgcg ttcttcccgg tgtgggcgaa
     2221 tatcatctca tcgatctcct tccgggtgcg ataggtgagg aataccggct cggcgttcgc
     2281 gttctgcgtc acgacgtggc gcgtgcggtc atcctccttg tccttgcggg tcagttcgtg
     2341 tttcttgatg aggtcggcgt tgtattcgtc ggccgacacg cagccgacga gcccggtctg
     2401 tacatggtcg cccatcttct gagcgtagat gtagaagagc ggctccttgt cctgcacgag
     2461 aataccctct cgtatgaagc gctgcagagt gtcacgtccc tgtgtgtaga cccggtcatc
     2521 gtggatatcg atatcacgtg gcaggtctat ctcggcccgc gtgatgtgga agaaggaatg
     2581 gggtttccct ttggcgaacg cggccgcctc ggcggtgttg acgacatcat agggtagcgc
     2641 ggcgatctcc tcgacgagcg atcggggggg gcggaaggcc ttgaaggggc gtacggtgac
     2701 catcgggtac tcctttcgaa cggttggtga tgcacgatac cggacggtta tagcgtcctt
     2761 cacggtcgct tgtcaagatt gtttttgttg acagcgtgtc gccgtgaagg catatacggg
     2821 agggacaacc tgcggcggag gatgccatgg cagataccac ctcgcgtatt attatcatcg
     2881 ggtcttcggc ggccggcctg tcggctgcgc aggaggcgcg gaaaacctct ccagaagcga
     2941 cgatcaccct catttcggaa gagccgcacc tgccgtacca tcgcccgtcg ctgaccgaat
     3001 acatcggcga cgagtcggtg gagcggcgcc cccaattcct gctccagccc gcggggtggt
     3061 tcgccgccca acggatagac ctccggcgcg gcattcgtgc cgccgggata gaccgccaga
     3121 gccggcaggt cctgctggta agcggggagc ttctgccgtt cgaccggttg gtactggccg
     3181 tgggagcgca gcctttcgtc ccgatggccg atgcgctgag ccgcgagaac gtttttgccg
     3241 tacggacgct cgacgacgcg cgccgcgtcg cgcagtgcgc agaccgctcc cac
//
LOCUS       DAOQUE010000327         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_33466_length_3286_cov_3.855463, whole genome shotgun sequence.
ACCESSION   DAOQUE010000327 DAOQUE010000000
VERSION     DAOQUE010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33466_length_3286_cov_3.855463"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKH10_09680"
     CDS             complement(<1..334)
                     /locus_tag="PKH10_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNT28435.1"
                     /translation="MTRIDSHAHLLTLITEKKIPADTLFGDMLYNGVTVCVNVILSPE
                     EARGRAMLDAACRDTGISLFHIAGVHPHDASGGVDGIAALLELPQVVAVGECGLDYHY
                     LRSPEAAQR"
     gene            complement(321..1784)
                     /gene="ricT"
                     /locus_tag="PKH10_09685"
     CDS             complement(321..1784)
                     /gene="ricT"
                     /locus_tag="PKH10_09685"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNT28436.1"
                     /translation="MSERKNHHHLNFDEQRELELLEKTKNEGFVDEEAITKNKKAGAP
                     IAPLGGSHAYYLDHSDPHPPFEEVLPLQFQPAGKVFWYHYPPLPTLSGDHQIQEGDHL
                     VAYSERGVEIAEVLKKSISDYNSQNKVDFIKHGVIRKATPEDMKKDENLRKKALEAQK
                     ICEQHNRDLGINMKLRKVKYTLDESKVIFYFTAPGRVDFRELIKRLASSLHRRIEMRQ
                     IGVRDTTKMLGGIGPCGQELCCRRYLHNFVPVSVKMAKDQNLSLNPNKISGCCGRLFC
                     CLGYEDPLYTELRRELPLEESEIFEAAPSGRRGFVKKVNVLNGTISVVFPSTEKENYE
                     ERLIQKSQLRREDRRWEIVPPAPSLKVESLAESRPPQQSRPAASTPAGEDRPRRDDRP
                     RRNDRDERPRNDRPRDNHPRDNNRPPAPASSGDQKDRPAPTADRPGGEQPQRQGDHRS
                     RRGGRNRHRPRPAGGGDRPAQQQQQQQQPPRQADDKN"
     gene            complement(1799..2599)
                     /locus_tag="PKH10_09690"
     CDS             complement(1799..2599)
                     /locus_tag="PKH10_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28437.1"
                     /translation="MEVSDIIIRAAARSVLPHAILFWGPLSQAEKTAVVLRLSHRLQG
                     LKRPLSLDEYSSRSVDGMLPDFKMITPDKGKDSERETVSVDQIERLAEEMMLYPFESA
                     NRIIYIPSAGCMTVPAQNTLLKKLEEPPANNFIIMDVAKPHLLLKTIISRCLSVYLPR
                     AKKEPDITFEDHLHWFSNNEEARKLVSDISVVFENNPLNSVSALSDIRSLLAAGSTLS
                     DREKILVLALFLKERAPQVAHDLIVFNENPQKTSMDSVFYYISQAMVT"
     gene            complement(2719..3174)
                     /gene="nusB"
                     /locus_tag="PKH10_09695"
     CDS             complement(2719..3174)
                     /gene="nusB"
                     /locus_tag="PKH10_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNT28438.1"
                     /translation="MGLRRRSREKTLQILYGMDFNKKGSYEEAVEEYYIYYNIPAACP
                     DAVSDEEERQFITYLLATVFDNRARIESLIEKAAINWRIDRIAFIERNILRLSVAELL
                     DEGNGIPFKATINEAVDIAKKFGNKESSSFVNGIINKVADMIDIKQRKL"
     gene            complement(3193..>3286)
                     /gene="ribH"
                     /locus_tag="PKH10_09700"
     CDS             complement(3193..>3286)
                     /gene="ribH"
                     /locus_tag="PKH10_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916359.1"
                     /note="RibE; 6,7-diimethyl-8-ribityllumazine synthase;
                     DMRL synthase; lumazine synthase; beta subunit of
                     riboflavin synthase; condenses
                     5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione
                     with L-3,4-dihydrohy-2-butanone-4-phosphate to generate
                     6,6-dimethyl-8-lumazine (DMRL); riboflavin synthase then
                     uses 2 molecules of DMRL to produce riboflavin (vitamin
                     B12); involved in the last steps of riboflavin
                     biosynthesis; forms a 60mer (icosahedral shell) in both
                     Bacillus subtilis and Escherichia coli; in Bacillus
                     subtilis this 60mer is associated with the riboflavin
                     synthase subunit (alpha) while in Escherichia coli it is
                     not; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNT28439.1"
                     /translation="RAGTKGGNKGFDAALAAIEMANLVENTNKI"
BASE COUNT          627 a          890 c          959 g          810 t
ORIGIN      
        1 cacgctgcgc cgcctcgggg gatcgcaggt agtggtagtc cagcccgcac tcccccaccg
       61 cgacgacctg ggggagctcc aggagggcgg cgatgccgtc gacaccccca ctggcgtcgt
      121 gagggtggac gccggcgatg tgaaagagcg atatgccggt gtcgcgacag gcggcgtcga
      181 gcatggcgcg gccacgggcc tcttcgggcg aaagtatgac attgacgcaa acggtgacgc
      241 cgttatagag catgtcgccg aacagggtat ccgccggtat ctttttctcg gtgatcagcg
      301 tcagaagatg cgcgtggctg tcaattcttg tcatcggcct gccggggcgg ctgttgctgc
      361 tgttgctgtt gttgtgcggg acgatctccg ccacccgccg gacgggggcg gtggcggttc
      421 cggccgccgc ggcgggaacg gtggtccccc tgccgttgcg gctgttctcc gccgggtcgg
      481 tcggcggtag gggccggccg gtccttctgg tcacccgatg atgccggtgc cggcgggcgg
      541 ttgttatcgc gagggtgatt gtcgcgggga cgatcattcc ggggccgttc gtcacgatcg
      601 ttccggcgcg gccggtcatc ccggcgggga cgatcttcgc ccgcaggggt cgatgcggcg
      661 ggccgcgact gttgcggcgg tctcgattcg gccaacgatt cgaccttgag cgacggagcg
      721 ggcgggacta tctcccagcg gcggtcctca cggcgcaact gcgatttctg gatgagccgt
      781 tcctcataat tctccttttc agtggagggg aacaccaccg atatggtacc gttcaacaca
      841 ttgacctttt tgacgaatcc gcgtcgaccg ctcggggcgg cctcgaatat ctcgctctcc
      901 tcgagcggca gttcccggcg cagttcggtg tagagggggt cctcgtaacc tagacaacag
      961 aacaggcgac cgcaacaccc cgatatcttg ttgggattga gcgaaagatt ctggtccttg
     1021 gccatcttga ccgagaccgg cacaaagttg tgcaggtagc ggcggcagca gagttcctgc
     1081 ccgcaggggc cgatgccacc cagcatcttg gtggtgtcgc ggacaccgat ctgtcgcatc
     1141 tcgatgcggc ggtggagact cgatgcaagc cgcttgatga gttcgcggaa atcgacccgg
     1201 ccgggggcgg tgaagtagaa tatgaccttc gattcatcca gcgtatactt gaccttacga
     1261 agtttcatgt tgatgccgag gtcgcggttg tgctgttcgc agatcttctg ggcctccagc
     1321 gccttcttgc ggagattctc gtcctttttc atgtcttcgg gggtcgcctt acgaatgacg
     1381 ccgtgtttga tgaaatcgac cttgttctgg gagttatagt cgcttatcga ctttttgagg
     1441 acctccgcga tctcaacgcc gcgttcgcta taggcgacca aatgatcgcc ttcctgtatc
     1501 tggtgatctc ccgaaagggt cggtagcgga gggtaatggt accagaagac cttgccggcg
     1561 ggttggaact gaagcgggag gacctcctca aaagggggat gcgggtcgga gtggtcgaga
     1621 taataggcat ggctgccgcc caacggggct atcggggcgc ccgccttttt gttcttggtg
     1681 atggcctcct cgtcaacaaa cccctcattc ttagtctttt caagcagttc cagttctctt
     1741 tgttcgtcaa aattgaggtg atgatgattc tttctttcgc tcatctatgg gtactccttt
     1801 aggttaccat tgcttgagat atataataga atactgaatc cattgatgtt ttctgcggat
     1861 tttcgttgaa aacgatcaga tcgtgcgcca cctgaggggc gcgttccttc agaaaaaggg
     1921 ccagcacgag aattttctcc cggtcggaga gggtcgatcc ggcggcgaga agtgagcgga
     1981 tatcgctgag cgcactcacg ctattaagag gattgttttc aaaaacgacg ctgatatctg
     2041 acacgagttt ccttgcttct tcgttattcg aaaaccaatg aagatggtct tcgaatgtta
     2101 tgtcaggttc tttttttgcc ctcggaaggt agactgagag gcaccgggag atgatcgttt
     2161 tcagcaaaag atggggcttc gcgacatcca tgatgatgaa attgttcgcg ggtggctcct
     2221 caagtttctt caaaagggtg ttctgggccg gcaccgtcat acagccggcg gacgggatat
     2281 agatgatgcg gttggcgctc tcgaacgggt agagcatcat ctcttctgca agacgctcga
     2341 tctgatcgac cgataccgtt tcacgctccg aatccttacc cttatctgga gttatcatct
     2401 tgaaatcagg caacattcca tcaacagagc gactcgaata ctcatcgagt gacagtgggc
     2461 gcttcagtcc ctgaagtcga tgagagagcc tcaggacgac cgcggtcttt tccgcctgcg
     2521 acagcggacc ccaaaaaaga atagcgtggg gcagaacgct tcgagcggca gctcttataa
     2581 taatatcaga tacttccata acttctgccg atcgggcctg ttaagtttac ataatatatg
     2641 ttatgcgaag ttgtcgccgg atgagcgata ctatccaagc ggttgcccca tcgagtcatc
     2701 atcatcatat ccggttccct agagtttcct ctgtttgata tcgatcatgt cggctacctt
     2761 gttgatgatg ccgttcacga acgacgacga ttctttgttc ccgaatttct tagcgatatc
     2821 gaccgcctcg ttaatggtcg ctttgaacgg gataccgttc ccctcgtcca gcagctcggc
     2881 caccgagagt ctgagtatat tccgctcgat gaacgctatc cggtctatcc gccagttaat
     2941 ggccgccttt tcgatgagcg actctatccg ggcccgattg tcgaacacgg tcgccagcag
     3001 atatgtgatg aattgacgct cctcctcgtc cgatacggca tcgggacaag cggccgggat
     3061 attgtagtag atataatatt cctccaccgc ctcttcgtag gatccctttt tattgaaatc
     3121 catcccgtaa aggatctgga gtgttttctc gcgcgagcgt cttctcagtc ccatctgctg
     3181 taaccctatc tgtcagatct tattggtgtt ctccacaagg ttggccattt cgatggcggc
     3241 aagcgccgcg tcgaagcctt tgttgccgcc cttggtgccc gcccgc
//
LOCUS       DAOQUE010000328         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_33547_length_3281_cov_3.931494, whole genome shotgun sequence.
ACCESSION   DAOQUE010000328 DAOQUE010000000
VERSION     DAOQUE010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33547_length_3281_cov_3.931494"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1008)
                     /gene="sppA"
                     /locus_tag="PKH10_09705"
     CDS             complement(25..1008)
                     /gene="sppA"
                     /locus_tag="PKH10_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNT28440.1"
                     /translation="MRYLRWSVVTFFLTLILTGCFQTQVKFFSDGSDPFREFVLEGAG
                     KQKIALIRVQGMITDQPEQGLLRSLPSVVQDTVAQLALAESDDAVRCVLLVVDTPGGT
                     VTASDMLYHEILRFKERSGKKVVVAMMNMATSGGYFISLPADRIVAHPSTVTGSVGVV
                     FMRPKLHGLMDKIGVSVEVDKSGDAKDTGSPFVPTTPEEKAFFQNIVDELAADFYAKV
                     IKHRKVSEAAMNEVRTAAVFLPRKAKELGLIDDIGYLDEATVAAKKAAGVGVDAQVVV
                     YRRTYYPNDSYYNNNVRPEGMKVPHLVNISLAGRDSIPAGFYYLWPLFNGE"
     gene            complement(1052..1498)
                     /locus_tag="PKH10_09710"
     CDS             complement(1052..1498)
                     /locus_tag="PKH10_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT28441.1"
                     /translation="MLTTVEKVLALKNVELFSDIPGEVLADIAFVIEEVTMDRGTYIV
                     HEGELGRELFIIVKGEVDVVSGDRKIDTIRQGGYFGEMAILDSQPRSADVVSLSDVTL
                     LKIDRDDFYEIMKQRDEVAIGIIKVLNRRIRNLTKQLVKNKSDNEV"
     gene            complement(1482..3215)
                     /locus_tag="PKH10_09715"
     CDS             complement(1482..3215)
                     /locus_tag="PKH10_09715"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT28442.1"
                     /translation="MTYLGVDHRFISIVVVIFGAAWITTILLLRKEYLLVLQDNIKKK
                     RLGNEDVTVRQNMIESIIVEAMTSENDDEVLMALDMIEKGRYFYLGRYFLPLLSEDVS
                     PKIKTKILRILRTMESRFYTFEILKLMKSGSDEVLKDAILTYGYMQMEKSHRYIATFL
                     ENPNLQILSAAVIALIKYCGISGIMTAAPYLKQLAESPDPERRVAAAYILGEIGQQNM
                     QQQVFEFLSDRDPQVRREAVKATTKMRSEVFIPKLFFMLLDKPVSLEVSKALAGFGER
                     IIEPAKSVLDNALENFELKMAVAKMLGEISSREVIPVLMDNLGDKSDELRNVLLESLK
                     RIMMRTDYHVLISQETLRRHLFREFYLYFQQLYYKSSMRAKTDLSYLSDVIDTKLLSC
                     LQRIFSLLSIMYGQKLFDSIYFNISQRYVSKEQRSTAVELVDNIVDKDIRRVLVPLIE
                     AIDEQEKLAQGFAAFKIKTPEYQHILEIFLTDDNDWVRAVTLHLIAVEEVVEMGDKVM
                     LFLYDPSPLVRESALFAAMRLGTSVSDDDVNFLLSDGAKNVSSYAAWYKAAGCQITFD
                     GTGGRSHAYDG"
BASE COUNT          683 a          956 c          913 g          729 t
ORIGIN      
        1 gggaacctcc gcgtaaacgg gcccctattc tccgttgaac agcggccaga ggtagtagaa
       61 gccggccggt atcgagtcgc gccccgcaag cgagatgttg acgaggtgcg gcaccttcat
      121 cccctcgggg cggacattgt tgttgtagta actgtcgttc gggtagtagg tgcgacgata
      181 aacgaccacc tgcgcgtcga cgccgacccc cgcggccttt ttcgccgcga cggtcgcctc
      241 gtcgagatac ccgatatcgt cgataagccc aagttctttc gcttttctcg gcaggaagac
      301 cgccgccgtc ctgacctcgt tcatggccgc ctcgctcacc ttccgatgct tgatcacctt
      361 ggcgtagaaa tcggccgcca gttcatccac gatgttctgg aagaacgcct tctcctccgg
      421 cgtcgtaggg acgaagggag acccggtatc cttcgcgtca cccgatttgt cgacctcgac
      481 cgacaccccg atcttatcca tgagtccatg cagtttaggt cgcatgaaca cgacgccgac
      541 cgagccggtg acggtcgaag gatgcgcgac gatacgatcg gccggtaacg agataaagta
      601 gccgccggat gtcgccatgt tcatcatagc cacgaccacc ttcttgcccg accgctcctt
      661 gaaacggagt atctcgtggt agagcatgtc gctcgccgtc acggtgccgc ccggcgtgtc
      721 cacgacgagc agtacacatc tcaccgcatc atcggactcg gcgagtgcca gttgggcgac
      781 ggtatcctgc acgacgctcg ggagcgaacg cagaagcccc tgttccggct ggtcggtgat
      841 catcccctgc acacggatga gcgctatctt ctgcttgccg gcaccttcaa gaacgaattc
      901 acggaacggg tcgcttccgt cgctgaagaa cttcacctgt gtctggaaac agcccgtcaa
      961 gatcagtgtc aggaaaaagg taacgaccga ccatcgaaga taacgcatga acagcctccg
     1021 tgggatgggt ccgccggatc gcgacgaccg gtcagacctc gttgtcactc ttgttcttga
     1081 ccaattgttt cgtcaggttg cggatgcgcc ggttgagcac cttgatgatg ccgatggcca
     1141 cctcgtcgcg ctgtttcatg atctcgtaga aatcgtcgcg atctatcttg agcagcgtca
     1201 catcgctcag cgacacgaca tcggccgaac ggggttgcga gtccagtatc gccatctcac
     1261 cgaaatagcc gccctgccgg atggtatcta tcttgcggtc gcccgatacg acatccacct
     1321 ctcccttcac gatgatgaac aattcccttc ccaactcgcc ctcgtggacg atataggtac
     1381 cacggtccat cgtcacctcc tcgatgacga acgcgatgtc ggccagcacc tcgcccggaa
     1441 tgtcgctgaa cagctcgaca ttcttgagcg cgagtacctt ttcaaccgtc gtaagcatgg
     1501 gatcgccctc ccgttccatc aaaggtgatc tgacagccgg cggccttgta ccacgccgcg
     1561 tagctcgaaa cattcttggc gccgtcgctg agcaggaaat tcacatcgtc gtcggagacc
     1621 gacgtaccga gccgcatcgc ggcgaacagg gccgattcac ggacgagcgg cgacggatcg
     1681 tacaggaaca gcatgacctt gtcacccatc tcgacgacct cttccaccgc gatgaggtgc
     1741 agggtcaccg cgcgcaccca atcgttgtcg tcggtcagga atatctcaag gatgtgctgg
     1801 tactctgggg tctttatctt gaacgcggcg aagccctgcg cgagtttctc ctgctcgtct
     1861 atcgcttcga tgagcggcac gagcacgcgc cggatatcct tgtcgacgat gttgtcgacc
     1921 agttcgaccg ctgtcgagcg ctgttctttc gaaacatagc gctgagagat gttgaaatag
     1981 atggaatcga agagtttctg gccgtacatg atggaaagga gcgagaatat ccgctggagg
     2041 cacgagagca gtttggtgtc tatcacatcg gaaagatagg acagatcggt ctttgcccgc
     2101 atggatgact tatagtaaag ctgctggaaa tacaagtaga attcgcggaa caggtgccgc
     2161 ctgagggtct cctgactgat gaggacatgg taatcggtgc gcatcatgat acgcttgaga
     2221 ctttcgagca ggacattgcg tagttcgtca gatttatcgc ccagattgtc catgagtacc
     2281 ggaatgacct cgcgcgagga tatctcgcca agcatcttgg cgaccgccat tttgagttcg
     2341 aaattctcga gcgcgttatc cagaacgctc ttcgccggct cgatgatgcg ctcgccgaaa
     2401 ccggccaacg ccttggacac ctcgagactc accggtttat cgagcaacat gaagaacagt
     2461 ttcggtatga agacctctga ccgcatcttg gtcgtcgcct tgaccgcctc gcggcgtacc
     2521 tgcgggtcac gatcgcttaa gaactcgaac acctgttgct gcatgttctg ctgaccgatc
     2581 tcgccgagga tataggccgc cgcgacgcgc cgttcgggat cgggcgattc ggccaactgc
     2641 ttgaggtacg gggccgccgt cataatgccc gatatgccgc agtatttgat gagcgcgatg
     2701 accgccgccg agagtatctg caggttcggg ttctcgagga aggtcgctat gtaccggtgc
     2761 gacttctcca tctgcatgta gccgtaggtc agtattgcat ccttgagcac ctcgtcgctc
     2821 ccgctcttca tgagtttgag tatctcgaaa gtgtagaaac gggactccat ggtccgcagg
     2881 atgcgcagta tcttggtctt tatcttggga gagacatctt cggacaggag cggcaggaaa
     2941 tagcgaccga gatagaaata acgccctttt tctatcatgt cgagcgccat gagcacttcg
     3001 tcgtcgttct ctgaggtcat cgcctcgacg atgatggatt ctatcatgtt ctgccgaacg
     3061 gtcacatctt cgttaccgag ccgtttcttt ttgatgttat cctgcaggac gagaaggtat
     3121 tctttccgaa gcaggagtat cgtggttatc cacgccgcac caaatatgac gacgacgatg
     3181 ctgatgaaac ggtgatcgac cccaaggtag gtcatcccgt agatggcgag gccggcgacg
     3241 ccggcgttga gtggcttgag gatgccgtct atcaccgcct a
//
LOCUS       DAOQUE010000329         3276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_33601_length_3276_cov_5.402360, whole genome shotgun sequence.
ACCESSION   DAOQUE010000329 DAOQUE010000000
VERSION     DAOQUE010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33601_length_3276_cov_5.402360"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PKH10_09720"
     CDS             complement(<1..343)
                     /locus_tag="PKH10_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HNT28443.1"
                     /translation="MTAEAAKLKEILALAAKIQASDIHLKTFSAPVFRINGQLVKVEE
                     FGPLKPEQIDGIVEEALNEDQRVQFKESSEVDLAYSLAGVGRFRVNVFRQRGSIGLVF
                     RIIPFSVKPLDE"
     gene            complement(347..1417)
                     /gene="recA"
                     /locus_tag="PKH10_09725"
     CDS             complement(347..1417)
                     /gene="recA"
                     /locus_tag="PKH10_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNT28444.1"
                     /translation="MDQKRKDVLESVIKGIEKQYGKGAVMVLGDETTQADIPVIPSGA
                     ISLDIALGIGGYPRGRIVEIYGPEGSGKTTLALHAIAEAQKRGGAAAFIDAEHAFDIT
                     YAKKLGIQVESLVVSQPDHGEQALEIAETLVRSNAIDVVVVDSVAALVPRSEIEGEMG
                     DSQVGVQARLMSQALRKLTGAVSKSKTCLIFINQLRMKIGVMFGNPETTTGGNALKFY
                     ASVRLDVRRIANIKEHEEVVGSQVRVKVVKNKMFPPFREAMFDLVFGKGIDRLADLVG
                     QAERDGVIQKSGSWYSYGDERLGQGAGNVAQALREKPELLKQIVAKVKDLHNLKVETE
                     PVEEQKAPKQEPAAKGRKKGGE"
     gene            1609..2301
                     /locus_tag="PKH10_09730"
     CDS             1609..2301
                     /locus_tag="PKH10_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNT28445.1"
                     /translation="MQERIEHLKKIWQAIGEAATLARRSPSGVRLVGVSKHKPASLIR
                     EFRRAGLTDIGESYVQEFLQKYEELRDEEIEWHFIGHLQSNKVKHIIDKVAWIHSVDS
                     LKLAEEIQKQAEKHDIVRVNILLQINVGGEDSKSGADADGAIALYRAVKDLDRLAVRG
                     LMTLPPFLGPEEVRPYFRQLRELRDRIISEEKADPQVFSELSMGMSGDFDVAIEEGAT
                     MVRLGTILFGER"
     gene            complement(2324..>3276)
                     /locus_tag="PKH10_09735"
     CDS             complement(2324..>3276)
                     /locus_tag="PKH10_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNT28446.1"
                     /translation="TAGDYCNGADAFSESCIPGEYLSCANGGSCNGSACDCPEPFAGP
                     TCEDCIEGYIYFSGDGGYCELDSDGDGYVNWDDNCPEIPNPDQTDSDYDGAGDECDCA
                     PLNDSCYPGGEGDWCDGKDNDCDGKVDEFMGEGPCDGPTIDYNGHTYLFLFSSGYYYD
                     NLCPWGYRPVVINGPAENTWIYETMTDFWGEEPPPYWIGLHYDMDRAAWRWDAIDQPA
                     YRKWASGQPYQEDGEECVEVRADGNGAPVWYATSCWNNDLWVCEACGNDSDGDGYGDL
                     CDCAPTDPAISPDADELLGNGIDDNCNGIIDEQGQGGDIY"
BASE COUNT          601 a         1024 c          975 g          676 t
ORIGIN      
        1 gttcgtcgag cggcttgacc gagaagggga tgatgcggaa cacgagcccg atactgccgc
       61 gctggcggaa gacattgacg cggaaccgac cgacgccggc gagcgagtag gcgaggtcga
      121 cctcgctgga ttccttgaac tgcacccgct ggtcctcgtt gagcgcctcc tcgacgatgc
      181 cgtctatctg ttccggcttg agcggtccga actcctcgac cttcacgagt tgcccgttga
      241 tgcggaacac cggggccgaa aaggtcttga gatggatgtc ggaggcctgt atcttcgccg
      301 cgagggcgag tatctccttg agtttagccg cttccgccgt catgcgctat tcccctccct
      361 tcttgcggcc tttcgccgcc ggttcctgtt tgggcgcctt ctgttcctcg accggttcgg
      421 tctcgacctt gaggttgtgc agatccttca ccttggcgac gatctgcttg agaagttccg
      481 gtttctcgcg caaggcctgc gccacattgc cggccccctg cccgagccgc tcgtcgccgt
      541 aggaatacca actgcccgat ttctgtatca cgccgtcgcg ctccgcctga cccacgaggt
      601 cggcgagccg gtctatcccc ttgccgaaga cgaggtcgaa catcgcctcg cggaacggcg
      661 ggaacatctt gttcttgacc accttgaccc gcacctgact gccgaccacc tcctcatgct
      721 ccttgatgtt ggcgatgcgc cgcacatcga gccggaccga cgcgtagaat ttgagcgcgt
      781 tgccgccggt ggtcgtttcg gggttgccga acatgacgcc gatcttcatg cgcaactggt
      841 tgatgaagat gagacaggtc ttcgacttcg acaccgcacc ggtgagtttc cggagcgcct
      901 gcgacataag ccgcgcctgc acgccgacct gactgtcgcc catctcgccc tctatctcgg
      961 agcgcggcac gagcgccgcg accgagtcga cgaccaccac atcgatggcg ttactgcgga
     1021 cgagcgtctc ggctatctcg agcgcctgtt cgccgtggtc gggttgcgac accacgagcg
     1081 actcgacctg tatccccagt ttcttcgcgt aggtgatgtc gaacgcgtgt tcggcatcta
     1141 tgaaggcggc ggcgccgccc cgtttctgcg cctcggcgat ggcgtggagc gcgagcgtcg
     1201 tcttgcccga cccttcgggc ccgtatatct cgacgatacg accgcgcggg tagccgccga
     1261 tgccgagcgc gatatcaaga cttatcgcgc cactcggtat gaccgggata tcggcctgtg
     1321 tcgtctcgtc gccgagcacc atgaccgcac ccttgccgta ctgcttttcg atgcctttga
     1381 tgaccgattc cagaacatct ttcctcttct gatccatggc cgaaaccccc ttgtggaata
     1441 aggttacctg ttcatatccc gtagccgacg gcatacctgc cggagcgatg atacccgacc
     1501 gggaacgaaa agcaacaggt cccccgtttt tttgtgcgac gcttcgcttc tccttgcaaa
     1561 gagggggcgt tcacgctagt atgcttcacc agatcggatg gagcgaccat gcaggaacgg
     1621 atagaacacc tcaaaaagat atggcaggcg ataggggagg cggcgacgct cgcgcgccgc
     1681 tctccttccg gggtacgact cgtcggcgtc tcgaaacaca aaccggcgtc gctcatccgc
     1741 gagttccgcc gtgccggact taccgacatc ggcgagagtt atgtacagga atttctgcag
     1801 aaatacgaag agttgcgtga tgaggagatc gagtggcact tcatcggtca cctccagtcg
     1861 aacaaggtga aacatatcat cgacaaagtg gcgtggatcc actcggtcga ctcgctgaaa
     1921 ctggccgagg agatacagaa acaggcggaa aagcacgata tcgttcgcgt caacatcctc
     1981 ctccagataa atgtcggcgg cgaggactcg aaaagcggtg ccgacgccga cggggcgatc
     2041 gccctctacc gcgccgtgaa ggatctcgac cggctcgcgg tgcgcggtct catgacgctc
     2101 ccgccgttcc tgggacccga agaggtgcgt ccctatttca ggcagttgcg cgaactacgc
     2161 gaccgcatca tcagcgagga gaaggccgat ccgcaggtct tttcggaact ttcgatggga
     2221 atgtcgggcg atttcgatgt cgccatcgaa gaaggggcga cgatggtgcg actcggcacc
     2281 atcctgttcg gggagcggtg acggtcttga aagacgatca gcgtcagtat atgtcgccgc
     2341 cctgtccctg ttcgtctatg atgccgttgc agttatcgtc gatgccgttg ccgagcagtt
     2401 cgtcggcgtc gggggagata gccgggtcgg tcggcgcgca gtcgcaaaga tcgccgtacc
     2461 cgtcgccgtc cgagtcgttg ccgcaggcct cacagaccca gagatcgtta ttccagcaac
     2521 tggtcgcgta ccagacggga gcgccgttgc cgtcggcacg gacctcgacg cactcttcgc
     2581 catcctcctg atacggctgt ccggaagccc acttgcggta ggcgggctga tcgatcgcgt
     2641 cccaccgcca agccgcacga tccatgtcgt agtgtaagcc gatccagtag ggtgggggct
     2701 cttctcccca gaagtcggtc atcgtctcgt agatccatgt gttctcggcc ggtccgttga
     2761 tgaccaccgg acgatacccc cacggacaca ggttgtcgta gtagtaccca ctggagaaaa
     2821 ggaacagata ggtgtgaccg ttgtagtcga tggtggggcc gtcacacggg ccttcgccca
     2881 tgaattcgtc gaccttcccg tcacagtcgt tatccttgcc gtcgcaccag tccccttcgc
     2941 cgccgggata acaggaatcg ttcaggggag cgcaatcgca ctcgtcgccg gcgccgtcgt
     3001 aatcgctgtc ggtctggtcg gggttgggta tctccggaca gttatcgtcc caattgacat
     3061 agccgtcgcc gtcgctgtcg agttcgcagt aaccaccgtc gcccgagaag tagatataac
     3121 cctctatgca gtcctcgcag gtcgggcccg cgaagggttc gggacaatcg cacgcggagc
     3181 cattgcagga accgccgttc gcgcaggaga ggtattctcc cgggatgcaa ctctctgaaa
     3241 aggcgtcggc cccgttgcaa taatcacccg cggtgc
//
LOCUS       DAOQUE010000330         3261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_33843_length_3261_cov_4.261697, whole genome shotgun sequence.
ACCESSION   DAOQUE010000330 DAOQUE010000000
VERSION     DAOQUE010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33843_length_3261_cov_4.261697"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKH10_09740"
     CDS             complement(<1..300)
                     /locus_tag="PKH10_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNT28447.1"
                     /translation="MRSFLLIFFFFFACSHHDTPPPAAPCSRIVSLAPSVTETLFELG
                     LGSRLVGVTRYCTWPPEALKIPKIGGWHDPSLEAVIGVRPDTVLHLPEQNGIAAKL"
     gene            complement(314..1099)
                     /locus_tag="PKH10_09745"
     CDS             complement(314..1099)
                     /locus_tag="PKH10_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNT28448.1"
                     /translation="MRIALIQNHPLFGEVENNINRLLTLIESVDADLYILPELCFTGY
                     QFVSREEVARLAVPPDGAVIGWFRQIAKERNVSIAFGYPELSPEGSYNAAMVVTPDGR
                     DLRYRKTHLFYREKEFFLPGDSGFGVIEWNGLRIGLAVCFDWYFPESFRTLALKGADI
                     IAHCSNLVMPYCQQADLTRALENRVYIATANRVGTEDRGGEALTFTGGSVLVSPRGEY
                     LLTLPKEEEVVMVAEIDIALAREKKINRYNDIIADRRPEFYNG"
     gene            1719..2180
                     /locus_tag="PKH10_09750"
     CDS             1719..2180
                     /locus_tag="PKH10_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNT28449.1"
                     /translation="MGENAPDFTLQDQFGADVKLSDLRGKKVLLSFHPLAWTPVCELQ
                     MRTLELKKKALDDLNTVPLGISVDSMYAKREWATFMELDQVQILADFWPHGEVAKKYG
                     QFIDKKGISGRVNILIDETGKIAWVKVYDLSQLPDIEEVLAVIRSKGAKKK"
     gene            complement(2182..>3261)
                     /locus_tag="PKH10_09755"
     CDS             complement(2182..>3261)
                     /locus_tag="PKH10_09755"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT28450.1"
                     /translation="VGFSSQIPTLFLAPLTGVAADRFPRRAILIATQSLFMTQALALA
                     AIVLLGVATIPIIILLSLFHGVVMAFDAPARHAFVVEIVGSRDDLSNGIALNSALFHA
                     SRLIGPSAAGILIMLWGEGVCFLANGLSYLTTIAALIAIRLTPAAKERERGRILSQFV
                     DGVRYARGSLPIKSILLLVAFVCFVGMPYTTLLPIIARDVLHGDSTTFGYLMSGAGIG
                     AVTGALYLASRPGGAGLLRNIAVAPVIFGVGAMALAFSRSFPLSFFILAVSAFGMMTV
                     MATSNTVLQEVVSERMRGRVMSLYTLSFFGSVPFGNLLAGSLAAHLGAPVTIFIAGIL
                     CVTAALVFRAYYAEIARGLASPSAN"
BASE COUNT          778 a          987 c          947 g          549 t
ORIGIN      
        1 cagtttcgcg gcgatcccgt tctgttcggg gagatggaga acggtatcgg gacgcacccc
       61 gatcaccgct tcgagcgacg gatcgtgcca gccgccgatc ttcggtatct tcagcgcctc
      121 ggggggccac gtacagtagc gcgtcacgcc caccagacgt gacccgaggc cgagttcgaa
      181 aagcgtctcg gtgacgctcg gggcgagcga cacgatgcgt gaacagggcg cggcgggcgg
      241 cggcgtgtca tggtgcgaac aggcgaaaaa aaagaaaaag atgagcagaa aagatcgcat
      301 caccccagaa cacctacccg ttatagaatt caggtcgtcg gtcagcgatg atgtcgttgt
      361 aacgatttat cttcttctcc cgtgcgagcg cgatgtctat ctcagcgacc atcaccacct
      421 cttcctcctt cgggagggtc agaagatatt cgccgcgcgg cgagaccaat acgctgccgc
      481 cggtgaaggt gagcgcctcc ccgccgcgat cctcggtgcc gacgcggttg gcggtcgcga
      541 tatagacccg gttctctaaa gcgcgagtga gatcggcctg ctggcagtaa ggcatcacga
      601 ggttagagca gtgggcgatg atatcggctc ccttgagcgc gagtgtccgg aaactttcag
      661 gaaaatacca gtcaaaacag accgcgagcc cgatacgaag gccattccac tcgatgaccc
      721 cgaatcccga atcgcccggc aggaagaact ccttttccct gtagaagagg tgcgtcttcc
      781 ggtatcgcag gtcacgaccg tcaggggtga cgaccatcgc ggcgttgtag gatccctcgg
      841 gcgaaagttc cggatagccg aatgctattg agacattacg ctcttttgcg atctgccgga
      901 accagccgat gaccgctccg tctggcggca ccgcaaggcg tgcgacctcc tcacgggaga
      961 cgaattggta accggtaaaa caaagttcgg gaagtatgta gaggtcagca tctaccgatt
     1021 ctattagtgt taacaatcta ttgatgttgt tttccacctc tccgaagagc ggatggttct
     1081 gaatgagtgc gatccgcatg aaaccccccg ttgtttacct tggccaccac cggtatatgc
     1141 gagaagtgac taaaagtaaa gaactctttt gtagctgaca ctttttcctc ttctcatttt
     1201 ctgacacaaa acctcccatt ttcaaaaata tcggaatttt tgttgagatt ttcggacagt
     1261 tgggtatgat gcactcgacc attgttgact gacattttgg caaggagttg attatggctg
     1321 acaaaaagaa gacacccgcc tcggtcaaag cgaaaaaacc ggctcccaag ccggccccca
     1381 aaccgatgaa acaggcgaaa ccggccccca agccggtcaa gacggcacaa aaaccagttc
     1441 aaaaacaacc ggttaaggta tcaaagcccg ctcccaaacc gatcccgccc aagccggtag
     1501 ccaagccggt cgccaagccg atctccccga aaccggtcac caaacccggc tccgccaagg
     1561 gctccgccgt ggcaagcccc gctcccaagc cggccccggc tcccaagccg gccccggccc
     1621 ctaaaccggc cccggctccc aagcccgccc cggcccctaa accggccccg gcccctaaac
     1681 ccgcacccgc gccgaagact gaacataagg gaccgaaagt gggcgagaat gctcccgatt
     1741 tcactctgca ggaccagttc ggcgccgatg tgaaattgag cgatcttcgc ggcaagaagg
     1801 ttctcctctc gttccatccg ctcgcgtgga ccccggtgtg cgaacttcag atgcggacac
     1861 tcgaactcaa aaagaaggcg ctcgatgacc tcaataccgt accgctcggg atcagcgtcg
     1921 attcgatgta tgcgaagcgt gagtgggcca cctttatgga actcgatcag gtccagatac
     1981 ttgccgactt ctggccacac ggcgaggtcg ccaagaaata cgggcagttc atcgacaaga
     2041 agggcatctc aggccgcgtg aacatcctca tagacgaaac gggaaagatc gcgtgggtga
     2101 aggtctacga tctgtcgcaa cttcccgaca tcgaagaggt gctcgcggtc atccgctcca
     2161 agggcgccaa gaagaaatga cctagttcgc cgacggcgac gccaatcccc tcgctatctc
     2221 agcgtaatac gccctgaaca ccagtgctgc ggtgacgcac aatatgcccg ctatgaagat
     2281 cgtcaccggc gctccgagat gtgcggcaag cgaaccggcg agcagattac cgaaagggac
     2341 cgagccgaag aacgagagcg tgtaaaggct catgacccga ccgcgcatcc gttccgacac
     2401 cacctcctgc aggacggtgt tcgaggtggc catgaccgtc atcatcccga aggccgagac
     2461 ggcaagaatg aaaaaagaga gcggaaaact gcgtgagaag gcgagcgcca tcgcacccac
     2521 cccgaatatc accggagcca ccgcgatgtt gcggagcagg ccggcaccac cgggtcggga
     2581 agcgagatag agcgcgccgg tgaccgcccc gatgccggcg cccgacatga gatacccgaa
     2641 ggtggtgctg tcgccatgca ggacatcgcg ggcgatgatc ggcagaagcg tcgtataggg
     2701 catcccgacg aaacaaacga acgcaacgag cagcaggata ctctttatcg gcagggatcc
     2761 acgggcatag cgcaccccgt cgacgaactg cgacagtatc cgcccgcgct cccgctcttt
     2821 tgccgcggga gtcagccgta tggcgataag cgccgctatc gtcgtgaggt aactcagccc
     2881 gttggcaagg aagcagaccc cctcacccca cagcatgatg agtatccccg ccgccgacgg
     2941 cccgataagc cgcgaggcgt ggaacagggc ggaattgagc gcgatgccgt tggaaagatc
     3001 gtcgcgcgaa ccgacgatct cgacgacgaa cgcgtggcgc gccggggcat cgaacgccat
     3061 cacgacaccg tggaacagcg acaggaggat gatgatcggt atcgttgcga cgccaagaag
     3121 cacgatggcg gcgagcgcga gcgcctgcgt catgaaaagc gactgggtcg cgataaggat
     3181 ggcgcggcga gggaaccggt cggcggcgac accggtcagc ggcgcgagga agagcgtcgg
     3241 tatctgggac gagaacccga c
//
LOCUS       DAOQUE010000331         3260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_33865_length_3260_cov_4.068019, whole genome shotgun sequence.
ACCESSION   DAOQUE010000331 DAOQUE010000000
VERSION     DAOQUE010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33865_length_3260_cov_4.068019"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PKH10_09760"
     CDS             complement(<1..1029)
                     /locus_tag="PKH10_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28451.1"
                     /translation="MRISFFLLLLCFAAVMTAQEANKGESTPVTPEPAVVETPAAPPA
                     EISTEAPTDATVPAPEEVPTEAPVEVSPVPTVEDRVKALEERQARLVEENGKLRQEIE
                     AKKKDAAEKEKVDIRKNRRPIEFRPYGFIELIGWAHDTLFNASEIPLYAKDDGKSTTG
                     MTAKRSRLGTKVFFPRLEGIDLTGMLEIDFFANMADTGFSESYPMIRMRHAFFDLGKT
                     WDDSTLGFKAGQTWAVAAPNVFPALINPGVGWGVGNLWQRMPLADIYFTQKFAKTFSF
                     TVDAAVARAMTGASANRNGFLEVNIDAGDASHIPQVQGQLSLKGAFSGLDLLVAVGGA
                     WGRESYKGG"
     gene            complement(1090..2508)
                     /locus_tag="PKH10_09765"
     CDS             complement(1090..2508)
                     /locus_tag="PKH10_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNT28452.1"
                     /translation="MMLPILLTFPLLAAVPLFSLRSRRAERSVLFLHALLTVGAVLYW
                     AFAGPEEFIGYFRADTGNLLFLALIAAVYLAVIPASYRYLEREKVSELWHRIYVVAML
                     LFVFSMTGAVLAKNLGLFWVFIEATTLASAPFIYFERHQSSLEATWKYVFICSVGIAI
                     AFIGILFLALSAKGVSDLFYTDLIANAPQLGRFWLLFSFPFLVVGLGTKAGVAPMHSW
                     LPDAHSEAPSPVSAFLSGMLLNTAFYGLYRIFLVMEAAGLRREAAIFFLVCGFASLLI
                     GAVYISGATNYKRLLAYSSIENMGIIFIGVALGGAGLFAAFLHLTAHALTKSSLFITA
                     GNIAHLFGTKEIAGVRGLREADRPTGALWGLGFAAIAGLPPFPIFISEFLIAREMVTQ
                     GRFGLLAAFLLLVTLVLYGMARAAFGMYLGSAPAAAKAGPSRPIDRLPEAALLTALLI
                     LGVAMPDAVRRIIDLATMSLLP"
     gene            complement(2505..3146)
                     /locus_tag="PKH10_09770"
     CDS             complement(2505..3146)
                     /locus_tag="PKH10_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28453.1"
                     /translation="MSQILTILFGVGILYLSLTSRMRTYIQVIALQGLILFLLIARDF
                     GELDWFTFFFLSFETLAFKTLIVPAVLWRIVERTHVARETDPHIPQFGSVVIGTAVFL
                     AGFAVTDWVSRIDPSVDALPFGIAVSTLIIAFFIILTRKKLITQVMGYMLMQNAVSLL
                     MIAMAVELPLLVNLGVLLDIFTLLFLFLLFLGKLHSAYEETSRERLTSLRDQP"
BASE COUNT          708 a          994 c         1049 g          509 t
ORIGIN      
        1 accgcccttg taactctcgc ggccccacgc gccaccgacc gcgacgagca gatcgagacc
       61 ggagaacgcg cccttcaacg acaactgtcc ctgcacttgc gggatatgcg acgcatcgcc
      121 cgcgtcgatg ttcacctcaa ggaacccgtt gcggttcgcc gaagcaccgg tcatcgcgcg
      181 cgcgacggcc gcatcgacgg tgaaggagaa tgtcttcgcg aacttctgcg taaaatagat
      241 gtcggcgagc ggcatccgtt gccacaggtt gcccacgccc cagccgacgc cggggttgat
      301 aagcgctggg aacacattcg gcgccgccac cgcccaggtc tggcctgcct tgaagccgag
      361 cgtcgaatca tcccaggtct tgcccagatc gaaaaaggcg tggcgcatgc ggatcatcgg
      421 gtaggattcc gagaagccgg tgtcggccat attggcgaaa aaatctatct caagcatacc
      481 ggtcagatct atcccttcga ggcgcgggaa aaaaaccttg gtgccgagtc gcgatcgttt
      541 cgccgtcatg ccggtcgtac ttttgccatc atccttcgcg tagagcggta tctcgctggc
      601 gttgaagagc gtatcgtgag cccatccgat gagttctatg aagccgtagg gccggaattc
      661 gatcggccga cggttcttgc ggatatcgac cttttccttc tctgcggcat ccttcttttt
      721 cgcctcgatc tcctgacgga gtttcccgtt ctcttcgaca agacgcgcct gacgctcctc
      781 aagcgccttc acgcgatcct cgacggtcgg tacgggcgac acttcgaccg gcgcctcggt
      841 cggcacctct tccggcgccg ggaccgtggc atcggtcggc gcttccgtcg agatctctgc
      901 ggggggcgcc gccggcgttt cgacgaccgc gggctccggg gtcaccgggg tcgactcccc
      961 tttgttcgcc tcctgagccg tcatgaccgc cgcgaaacaa agcaagagca ggaaaaaaga
     1021 gatcctcatg tgttccctcc gtcaattcct caaaggaccg caccacccta taggatataa
     1081 aggctcttgt caaggcagga gagacatcgt ggcgagatcg atgatccggc gcaccgcatc
     1141 gggcatcgcc acgccgagga tgaggagcgc cgtcaggagc gccgcctccg gtagccgatc
     1201 gatcggccgg gacggtccgg ccttcgccgc cgccggagcc gatccgagat acatcccgaa
     1261 cgcggcacgc gccatgccgt aaaggacgag cgtcactagc agcaggaacg ccgcaagcag
     1321 gccgaaacgc ccctgcgtca ccatctcgcg ggcgatcagg aactcggaga tgaatatcgg
     1381 gaacggcggc aggcccgcga tcgcggcgaa cccgaggccc catagcgcgc cggtcggccg
     1441 gtcggcctcg cgcagcccgc gcaccccggc gatctccttg gtgccgaaga ggtgggcgat
     1501 gttcccggcg gtaatgaaca gagaactttt cgtgagcgcg tgcgcggtca ggtgcaggaa
     1561 cgcggcgaac aggccggcgc cgccgagcgc cacgccgatg aagatgatcc ccatgttctc
     1621 tatggagctg taggcgagca gccgtttgta gttggtcgcg ccgcttatgt agaccgcgcc
     1681 gataagaagt gatgcgaacc cgcagacaag gaagaatatc gccgcctcgc gccgcagtcc
     1741 cgccgcctcc atcaccagaa agatgcggta gagcccgtaa aaggcggtat tcagcagcat
     1801 ccccgacagg aacgccgaca ccggcgaggg ggcctccgaa tgtgcgtcgg gaagccacga
     1861 atgcatcggc gcgacgcccg ccttggtgcc gagcccgacc accaggaacg ggaacgagaa
     1921 cagcagccag aaccggccca gttgcggcgc gttggcgatg aggtcggtat agaaaaggtc
     1981 ggacaccccc ttcgccgata gcgcgaggaa gaggatcccg atgaaggcga tcgcgatgcc
     2041 gaccgagcag atgaagacat atttccacgt cgcctcgagc gacgactggt gccgttcgaa
     2101 gtagatgaag ggggccgacg cgagcgtggt cgcctcgatg aatacccaga acagtccgag
     2161 gttcttcgca agcaccgcgc cggtcatcga gaagacgaac agcagcatcg ccaccacata
     2221 gatgcggtgc cacagttccg acaccttctc ccgctcgagg taacggtaac tcgccgggat
     2281 gacggccaga tagaccgccg cgatgagggc gaggaacagc aggttgccgg tgtcggcgcg
     2341 gaagtagccg atgaactcct cgggaccggc aaaggcccag tacaggaccg cgccgacggt
     2401 gaggagcgcg tggaggaaaa gcaccgaccg ttcggcgcgt cgcgaacgga gcgaaaagag
     2461 cggcaccgcg gcgagcagcg ggaaggtgag gagtatcggc agcatcatgg ctgatccctc
     2521 agactcgtca atctctcgcg agaggtctcc tcgtaggccg aatggagttt cccgaggaac
     2581 agcaggaaga ggaagagcag cgtgaagatg tcgagcagca ccccgagatt cacgagcagc
     2641 ggcaattcca ccgccatcgc gatcatgagg agcgacacgg cgttctgcat gagcatgtag
     2701 cccatcacct gcgtgatcag tttcttgcgc gtcaggatga tgaagaaagc gatgatgagc
     2761 gtcgagacgg cgatcccgaa ggggagcgcg tcgaccgacg ggtcgatgcg cgacacccag
     2821 tcggtcacgg cgaaacccgc aaggaacacg gccgtgccga tcacgaccga tccgaactgc
     2881 gggatgtgcg gatcggtctc acgcgccaca tgggtccgct cgactatgcg ccagagcacc
     2941 gccggaacga tgagcgtctt gaaggcgagc gtttcgaaag agaggaaaaa gaaggtgaac
     3001 cagtcgagtt ccccgaaatc gcgcgcgatc aggaggaaca ggatgagacc ctgcagagcg
     3061 atgacctgta tgtaggtgcg catgcggctg gtgagggaga gatagaggat gccgacgccg
     3121 aacaagatag tgagtatctg actcatgcga cccctccgcg gaaatagacc gacgcggcca
     3181 gcgcgatgag cgcgagagcg acggtgagga agaggaactg cggcacatgc gatatgcgga
     3241 agcgggcgac gagcgattcg
//
LOCUS       DAOQUE010000332         3258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_33896_length_3258_cov_4.877927, whole genome shotgun sequence.
ACCESSION   DAOQUE010000332 DAOQUE010000000
VERSION     DAOQUE010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33896_length_3258_cov_4.877927"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PKH10_09775"
     CDS             <1..1154
                     /locus_tag="PKH10_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28454.1"
                     /translation="DRNDRYDAPPQEHRSWDRDREREKERQQQHHQPVAQRPAPQPEP
                     RPDMRPPAPVEQKKAEPPVTPPPAPAPQPVIVKSQPVKEEWIAPIIEEPIEKIQVTVI
                     TQDIVIPHEFARTLQPLPLNEYTLYIKLLLYSFSIHKNYGYLGAGLKKSVGLDELAQD
                     EFSRLLDRLCKRGLAVVEKISEYQSTYALYLPFDPETMKKVDEKKKDEPEAEPEKAPR
                     AKEKEPARPEMQKQPDRQQQEGRPRGGKKEEPKKGKDTGRDAKKAKEPEKKQPPKGRG
                     RPPVKERPAPAEEPVEEMIPGRTAIHLDDDGLMKAYRTFVSMEIDKAKMRVGRSNFDK
                     IYMEAVKYIDKKHGFKVLSDQERFREYLTQYYISAFDIPDFEAWKKAKG"
     gene            1156..1389
                     /locus_tag="PKH10_09780"
     CDS             1156..1389
                     /locus_tag="PKH10_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28455.1"
                     /translation="MDIRIFMKSLNDIDIKLMLCKSNFQWKLLAEKYDEAERKIQEFY
                     GAKIPNKLTGALGRVRLQLVEKKGHLPPEDILV"
     gene            1412..1834
                     /locus_tag="PKH10_09785"
     CDS             1412..1834
                     /locus_tag="PKH10_09785"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNT28456.1"
                     /translation="MNKLLNISERVNIAIHALAYIAASDRNASVKRMAKDLKVSETYL
                     AKVLQPLVKEKLIRSKRGARGGFTLAKKPSEITAYDLVTVIEGPLPTNTCLFLEPICH
                     DENCRFRSLTGEVRALLEVSLRKIRVSDIMKNFDGLKK"
     gene            1989..>3258
                     /locus_tag="PKH10_09790"
     CDS             1989..>3258
                     /locus_tag="PKH10_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNT28457.1"
                     /translation="MTAPQSLLNLIERFEEQLDSYKSGDYKEASLRREFLDPFFKALG
                     WDIDNEQGYAEAYKDVVHEDTLRIGGAVKAPDYGFRIGGVRKFFLEAKKPSVAVKDDI
                     SAAYQLRRYAWSAKLPLSILSDFEEFAVYDCRVRPNPADKAATARVFYCTFREYAEKW
                     DWLYATFSREAILKGSFDRYAETNKGKRGTAEVDADFLRTIESWRNDLAHNLAIRNAA
                     LTQRELNFAVQRTIDRIIFLRICEDRGIEDYGRLLALSNGGDIYKRLGMIFREADARY
                     NSGLFHFKAERDAAEPPDEITLALSLDDKLLRDMLKSLYFPDSPYEFSVLPADILGHV
                     YEQFLGKVIRLTDGHRAVVEEKPEVRKAGGVYYTPTYIVEYIVKETVGKLTAGKTPKE
                     MAALRILDPACGSGSFLIGAYQYLLDRHFAW"
BASE COUNT          822 a         1028 c          898 g          510 t
ORIGIN      
        1 ccgatcggaa cgaccggtac gatgcccccc cgcaagagca ccgttcgtgg gatcgcgacc
       61 gcgagaggga aaaggagcgt caacaacagc accaccagcc ggtcgcacag agaccagccc
      121 cccagcccga accccgtccc gacatgcgcc ccccggcgcc cgtcgagcag aaaaaggccg
      181 aaccgcccgt cacgccgccg cccgcccccg cgcctcagcc ggtcatcgtc aaatcccagc
      241 cggtcaagga ggaatggata gcgccgatca tcgaggagcc gatagagaag atacaggtca
      301 cggtcatcac gcaggacatc gtcatcccgc atgaattcgc ccgtaccctt caaccgctcc
      361 cgctgaacga atatacgctc tacatcaagc tgctcctcta ctccttcagc atccacaaga
      421 actacggtta tctcggcgct ggactcaaga agagcgtcgg gctcgacgaa ctggctcagg
      481 acgaattctc gcgcctgctc gacagactct gtaaacgggg actcgcggtc gttgagaaga
      541 tatcggagta ccagagcacc tacgcactct accttccctt cgatcctgag accatgaaaa
      601 aggtcgacga gaagaaaaag gacgaaccgg aggcggagcc ggaaaaggcg ccccgcgcaa
      661 aggagaagga acccgcgaga ccggaaatgc agaaacagcc ggacaggcag caacaggagg
      721 gcaggccgcg cggcggcaaa aaagaggaac cgaagaaagg aaaggacacc ggccgcgacg
      781 cgaagaaagc gaaagagccg gagaagaaac agccgccgaa aggacgggga cgcccgccgg
      841 tgaaagagag accggcgccc gccgaagagc cggtcgaaga gatgataccc ggcagaacgg
      901 cgatacatct ggatgacgac gggctcatga aggcgtaccg caccttcgtt tcgatggaga
      961 tagacaaggc gaagatgcgg gtcggccgct ccaacttcga caagatatac atggaggcgg
     1021 tcaagtacat cgacaaaaaa cacgggttca aggtgctctc ggatcaggaa cggttccgcg
     1081 aatacctgac ccagtactac atctcggcct tcgacatccc cgatttcgag gcatggaaaa
     1141 aagcgaaggg ctaggatgga catccgcatc ttcatgaaat cactgaacga tatagacatc
     1201 aaactgatgc tctgcaaaag caacttccag tggaaactcc tcgccgagaa gtacgacgag
     1261 gcggaacgga agatacagga attctacggc gcgaagatac cgaacaaact aaccggcgcc
     1321 ctcggccgcg tccgattgca actcgtggag aaaaaagggc acctgccgcc ggaagacatt
     1381 ttagtgtgat catagaaaag agggcacggc catgaacaaa ctgctgaaca tctcggaacg
     1441 ggtcaacatc gcgatccacg cgctcgccta catcgcggca tccgaccgga acgcgtcggt
     1501 caagcggatg gcgaaggacc tcaaggtgtc ggagacctat ctcgcgaagg tgctccagcc
     1561 gctcgtcaag gaaaaactca tccgttcgaa acgcggcgca cgcggcggct tcacgctcgc
     1621 caagaaaccg agcgagataa ccgcttacga tctcgtcacc gtcatcgaag ggccgctccc
     1681 gaccaacacc tgcctttttc tcgaaccgat ctgtcacgat gagaactgcc gtttccgttc
     1741 gctgaccggc gaagtccgcg cgctcctcga agtgtcgctc cgcaagatac gagtgagcga
     1801 tatcatgaag aatttcgacg gcttgaagaa atagcgggaa ctctctcccg cgccgcgccc
     1861 tctttccgcg acctcccccg ccgggacacc cgatgtcccc tttcccgtga tataacgacg
     1921 gtggtggaat tatttgaaaa gggacatgaa aagggatagg ataagccgtc ttgaaaaacg
     1981 aggggaacat gaccgcgcca caaagccttc ttaacctcat cgaacggttc gaggaacaac
     2041 tcgattccta caaatcgggc gactacaaag aggcgtcgct ccggcgcgaa ttcctcgacc
     2101 cgttcttcaa agcgctcggc tgggacatcg acaacgaaca ggggtacgcc gaggcgtaca
     2161 aggatgtggt gcatgaagac accctgcgga tcggcggcgc ggtcaaagcg cccgactacg
     2221 ggttccgcat cggcggggtc cgcaaattct tcctcgaagc gaaaaaaccg agcgtcgcgg
     2281 taaaagacga catttcggcg gcataccaac tgcgccgcta cgcatggagc gccaaactgc
     2341 ccctttccat cctttccgac ttcgaagaat tcgccgtcta cgattgccgc gtccgcccca
     2401 atccggccga caaggcggcc accgcccgcg tgttctactg caccttccgc gaatacgccg
     2461 aaaaatggga ctggctctac gcgaccttct cgcgcgaagc gatactcaaa ggttcgttcg
     2521 accgctacgc cgaaacgaac aaagggaaac ggggcacggc ggaagtggac gccgatttcc
     2581 tgcgcaccat cgagtcgtgg cgcaacgacc ttgcccacaa tctcgccatc cgtaacgcgg
     2641 cgctcaccca gcgcgaactc aacttcgccg tccagcgaac catcgaccgg atcatcttcc
     2701 tgcgcatctg cgaagatcgc ggcatcgagg attacggccg acttctcgcc ctctccaatg
     2761 gcggcgacat ctacaaacgg ctcgggatga tattccgcga ggccgacgcc cgctacaatt
     2821 cggggctctt ccacttcaag gccgaacgcg acgccgcgga gccgcccgac gagataaccc
     2881 tcgccctttc cctcgacgac aaactgctcc gcgacatgct gaaaagtctc tacttccccg
     2941 atagccccta tgagttcagc gtccttcccg ccgacatcct cggccatgtc tacgaacaat
     3001 tcctcggcaa ggtcatccgc ctcaccgacg gtcaccgcgc cgtggtcgaa gagaaaccgg
     3061 aagtgcgcaa ggcgggtggc gtctactaca caccgaccta catcgtggaa tacatcgtca
     3121 aagagacggt ggggaaactg accgccggaa aaacgccgaa ggagatggcc gctctgcgca
     3181 tcctcgatcc ggcgtgcggt tccggttcgt tcctgatcgg cgcgtaccag tatctgctcg
     3241 accgccattt cgcgtggt
//
LOCUS       DAOQUE010000333         3240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_34203_length_3240_cov_4.204710, whole genome shotgun sequence.
ACCESSION   DAOQUE010000333 DAOQUE010000000
VERSION     DAOQUE010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34203_length_3240_cov_4.204710"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PKH10_09795"
     CDS             <1..621
                     /locus_tag="PKH10_09795"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNT28458.1"
                     /translation="LSLDVPIAGVNAGSLGFLTEITPDEPDELRRVLGPSPLIQRRIA
                     LSVTVLRGEEKVLTAVAVNELLVMRHPDGTMLDFAIRYAAMNLPHYKADGLIVATPTG
                     STAYNLSLNGPILFPTEHAMVVNAVAPHALTQRPLVLSSASELEISLGEGETATLSVD
                     GGRPVALGSGDRIVAREMHRMLKFVPSSRRNFFDVLARKLHFGRRD"
     gene            685..981
                     /gene="raiA"
                     /locus_tag="PKH10_09800"
     CDS             685..981
                     /gene="raiA"
                     /locus_tag="PKH10_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNT28459.1"
                     /translation="MNINFSFKGLDHSDGLKEYAERRLTKLERLITQNTQVEVFFVKD
                     RNEKKTEIKLNHKGEDYFAAESSEEFDQSIDFCVDKISKQIKKSKEKRIDRRGR"
     gene            997..1073
                     /locus_tag="PKH10_09805"
     tRNA            997..1073
                     /locus_tag="PKH10_09805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Arg,seq:ccg)
     gene            1105..1938
                     /locus_tag="PKH10_09810"
     CDS             1105..1938
                     /locus_tag="PKH10_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNT28460.1"
                     /translation="MPSRSDGAELYVKSAAEVSTAFDLVSYRLDEPSVPPLFCAYMPE
                     DMTELSDRERVSLFIRTLLPLILRENERIEQESRRLDEAAARSFAGTGDAALVYDLAV
                     KYRVAEEGGGPLNEGELGTVTEALRSRIRPVAPSIVLTIAALESGWGTSRFTREANNL
                     FGHTALLAWRGIQPAKWRGTTRNIKRFETIADALRRFMLNLNSNRAYARYRKYRDIAP
                     ADTFQQTKGFTTYASIGKEYVVRMQQVMKRFGFARYDGHRLADDTRLWGLDRLRTPYL
                     Y"
     gene            1977..2387
                     /locus_tag="PKH10_09815"
     CDS             1977..2387
                     /locus_tag="PKH10_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28461.1"
                     /translation="MLDRSCYDIARDPRVADKTILKLEYKKFNTFLFDYLRSLNRGWI
                     FMKMKHKYPVGTVLHFVMSVNGMDEPLKLSGTVVHHGVNDEGKEGVGVRLTVDDGASA
                     DLRRRIGEHARTLFGEQLAGRILAIAEAQDRGAE"
     gene            2374..3192
                     /locus_tag="PKH10_09820"
     CDS             2374..3192
                     /locus_tag="PKH10_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT28462.1"
                     /translation="MARNKTPERGDAKFEERARLLVHADRLATVGTMVSSIVHEVNSP
                     LTFIKANQETALIAIRQVLERCDDPEIRRILEELVMPSLTDSVAGVRQIEQLLNSFRR
                     FYKQEKTVMAIDLHAIIEDVRILTAYHLRKEKVALTVDVPPADPLLVWGNKQELMQAL
                     TNVINNALDAFENCSVAQRRIALTIERQGDMLVMTVANNGPAIPQELSESIFDSFFTT
                     KNEERGTGLGLATVRQIMRGMGGDIVLKDRGGKDGLVAFACTMPAYRGEAAQIG"
BASE COUNT          647 a          979 c         1001 g          613 t
ORIGIN      
        1 ctttcgctcg atgtccccat cgccggggtg aacgccggaa gtctcgggtt cctgaccgag
       61 ataacgcccg acgagcccga cgaactgcgg cgcgtgctcg gaccgtcgcc cctgatacag
      121 cggcgaatcg cgctctcggt gaccgtcctg cgcggggagg agaaggtcct gaccgccgtc
      181 gcggtcaacg aactgctcgt catgcggcat cccgacggga cgatgctcga tttcgccatc
      241 cggtacgcgg cgatgaatct cccgcactac aaggccgacg ggctcatcgt cgcgaccccg
      301 accggctcga ccgcctacaa tctctcgttg aacgggccga tactgttccc gaccgagcac
      361 gcgatggtgg tcaatgcggt ggcgccgcac gcgctcaccc agcggccgct cgtcctgtcc
      421 agcgcctccg aacttgagat atcgctcggt gaaggcgaga cggcgacgct ctcggtggat
      481 ggcggcaggc ccgtggcgct tggatcgggt gaccgcatcg tggcacgcga gatgcaccgg
      541 atgctcaagt tcgttccgag ttcccgccgc aactttttcg atgtgctggc gcggaaactg
      601 catttcggcc gtcgcgatta gttgacagga taccgccggc atgctaccat tgttccgtac
      661 tttttctttt gaaaggagga aaccatgaac atcaattttt ccttcaaggg ccttgaccac
      721 agcgacggtc tcaaggagta tgctgagagg cgtctgacca aactggagag actcatcacc
      781 cagaatacgc aggtggaggt gtttttcgtc aaggacagga acgagaagaa gaccgagata
      841 aaactgaacc acaaggggga agattacttc gcggctgagt cgagcgagga gttcgaccag
      901 tcgatagatt tttgtgttga caagatctcc aaacagatta agaagtccaa ggagaagagg
      961 atcgaccgcc gcgggcggta gttccggtcg ttagttgggc ggatagctca gttggataga
     1021 gtgttggcct ccgaagccaa aggtcgtggg ttcgatcccc gctccgccta ccatcttctt
     1081 ttctgttccc tcctcatctt cttcgtgccg tcgcgatcgg atggtgccga actctatgtc
     1141 aaaagcgccg ccgaggtgtc aaccgcgttc gacctcgttt cctatcgtct cgacgagccg
     1201 tcggtgccgc ctcttttctg tgcctatatg cccgaggaca tgacggaact gtccgatcgg
     1261 gagcgggtgt cgctcttcat ccggaccctg ttgccgctca tcctgcgcga gaacgagagg
     1321 atagagcagg aatcccgccg gctcgacgag gcggccgccc ggtcgttcgc cggcacggga
     1381 gatgccgcgc tcgtgtacga tcttgcggtg aaataccgcg tggcggagga gggcggcggt
     1441 cctctgaacg agggggaact cggaacggtc accgaggcgc tgcggtcccg gatcaggccg
     1501 gtcgccccct cgatcgtcct caccatcgcg gcgctcgaat cgggttgggg gacatcgcgt
     1561 ttcacccgcg aggcgaacaa tctgtttggc cacaccgcgc ttcttgcgtg gcgcggcata
     1621 caaccggcga aatggcgcgg gacgacccgt aacataaaac ggttcgagac catcgccgac
     1681 gcgctcaggc gtttcatgct caatctcaac agcaatcggg cctacgcgcg ctaccggaag
     1741 tatcgcgaca tcgcgcccgc cgacaccttt cagcagacga aggggttcac cacctacgcg
     1801 agcatcggca aggagtatgt cgtgcgcatg cagcaggtca tgaaacgatt cggcttcgca
     1861 cgttacgacg ggcatcgtct tgccgacgat acgcggctgt gggggctcga tcggctccgg
     1921 accccgtacc tttactagcc gccgcgagaa aattgacagc gcccgaccgg gcgggtatgc
     1981 tcgaccgttc ctgttacgat atcgcgaggg atccccgcgt ggccgacaag acgatactga
     2041 aactcgaata caagaaattc aacaccttcc tcttcgatta cctgcggtca ctcaaccgcg
     2101 gctggatatt catgaagatg aagcacaagt acccggtcgg cacggtcctc cattttgtca
     2161 tgagcgtgaa tggcatggac gagccgctga aactgtccgg gaccgtcgtg catcacggcg
     2221 tgaacgacga gggtaaagag ggtgtcggcg tgcgtctgac cgtcgacgac ggcgcgtccg
     2281 ccgatctgcg gcggcgtatc ggggagcatg cccgcacatt gttcggcgaa cagctggccg
     2341 ggcgcatcct ggcgatcgcg gaggcgcagg atcgtggcgc ggaataagac cccggagcgg
     2401 ggcgatgcga agttcgagga acgggcccga ctgctcgtgc acgccgaccg gctcgctacg
     2461 gtcggcacca tggtcagttc catcgtgcac gaagtgaatt cgccgctcac cttcatcaag
     2521 gccaatcagg aaacggcgct tatcgcgatt cggcaggtgc ttgagcggtg tgacgacccg
     2581 gagatccgcc gcatactcga agaactcgtc atgccctcgc tcaccgactc cgtcgccggc
     2641 gtccggcaga tagagcagtt actcaactcg ttccggcgtt tctataaaca ggaaaaaacg
     2701 gtcatggcga tagacctcca cgccatcatc gaggatgtgc ggatactcac cgcgtaccac
     2761 ctgaggaaag agaaggtcgc tctcacggtc gatgtcccgc cggccgaccc gctcctcgtg
     2821 tggggcaata agcaggaact catgcaggcg ctgaccaatg tcatcaataa tgcgctcgac
     2881 gcattcgaga actgttcggt ggcccagcgg cgtatcgcgc tcaccatcga gaggcagggc
     2941 gacatgctgg tcatgaccgt tgcgaacaac ggtcctgcga taccgcagga actctccgag
     3001 agcatcttcg attccttttt caccaccaag aacgaggagc gcggcaccgg actcgggctc
     3061 gccaccgtac ggcagatcat gcgcggcatg gggggtgata tcgtcctgaa ggaccggggc
     3121 gggaaggacg ggctggtcgc cttcgcctgt acgatgcccg cctaccgcgg ggaggcggcg
     3181 cagatcggat gagtctttcc tccatcatac tggcggcggg caaggggacc cgtatacgtt
//
LOCUS       DAOQUE010000334         3229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_34376_length_3229_cov_4.559546, whole genome shotgun sequence.
ACCESSION   DAOQUE010000334 DAOQUE010000000
VERSION     DAOQUE010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34376_length_3229_cov_4.559546"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3229
                     /locus_tag="PKH10_09825"
     CDS             <1..>3229
                     /locus_tag="PKH10_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28463.1"
                     /translation="ITDDLIPDEEEPDEADIDTKPDPDKVEADIVDGDIADVEPDDDI
                     VTGPCAPNPCEEMTGSTGECTVDGDTFICGCAANYTWNAEEKECAANMRRTNCANIPQ
                     NAHGIGDNADGKYEQTWNGEAWLPASVVCTWECDNNYSLNEEETGCLADIQRTDCTNI
                     PDNAHGVLANADGKFQQVWNGESESWEPASFTCAWVCDDNYTKNGEVCDANTRRVACV
                     NSLPDNAHWADPNADGKFLQTWNGSTDEWEPLTSVCAWACDENFSLNDEEDGCVANTR
                     RTDCVSAKPDHSHYSGENDDDKFEQTWDAGSNDWTPAFFTCKWDCDDNYTFDSMGPTC
                     VPDTRPEECTNTLPDNAYWIDPNADGTFEQTWDGDSWEPATFSDCAWECDEHYTQNGL
                     VCDPKTQRADCVNQIPDHAFYSGANDDGKFEQTWDGGIEDWTPAPETVTCEWECDKGY
                     PINEAEDDCVFQPIVYVNHAATDGENTGYSWADAFTDLQDALKQVIAGQEIWVATGTY
                     KPSRCPYFDGCDDKEKTFMMFRDTGVDFVLYGGFDGTETTRDQRDWETNETILSGDIT
                     GDDAWDDVNGGWLNRTDNVYRVLVFDPAMPSNTLLLDGFTVSGGHSEGMDNRGGAVSS
                     TNHAVEVRNCVFRGNVASGGGAIFVIDGQLTVDNSFFYRNAADPSSSGSGGAIATLNA
                     PLSLTDSVFDENFAGDNAGAVNCNGNVTISGSSFLNNHAAGNAAALSIGNATLATITD
                     TDFVGNTSGFGGAIGINTTTSVVVASCYFEANEATDENGSGGAISVNADSFSLSNSQF
                     VDNSASMGSAVKLWTVADATVTSVTFTGNTGSAISAQGGALVITNSTFTDNTAAVNRV
                     SAIEMQDASSLSISSSSFLSNEADRSTINVRDTLLFSVNDCSFQNNNGSAINMEGTPL
                     QMTDCDFIGNHAASGAAVNPGDGDHTITNCRFKNNAADLPGEPDDIFAGWGGVMAMMG
                     ANMTVINSVFEGNTAAESGGAVAAIWSTLSFTNCTFTGNTDLNGDGIVYIGTNGPVNN
                     TILWDTVEEVTTLTVGEDVYDMGPSNPTFSYSDVYGSGGSGNDCGP"
BASE COUNT          736 a          940 c          922 g          631 t
ORIGIN      
        1 atcaccgacg acctcatccc cgatgaagag gagccggatg aggccgacat cgacacgaag
       61 ccggatcccg acaaggtcga ggccgacata gtggacggcg atatcgccga tgtcgaaccc
      121 gacgacgata tcgtgaccgg gccctgtgcc ccgaatccct gcgaagagat gaccgggagc
      181 acgggcgaat gcaccgtgga cggcgacacc ttcatctgcg gctgtgcggc gaattacaca
      241 tggaatgccg aagaaaaaga gtgcgcggcc aacatgcggc gaacgaactg cgcgaacatc
      301 ccgcagaacg cgcacggtat cggtgataac gccgacggca agtacgaaca gacatggaac
      361 ggcgaggcgt ggcttcccgc gtcggtcgtc tgtacttggg agtgcgacaa taactattcg
      421 ctgaacgagg aagagaccgg ttgtctcgcc gatatccagc ggaccgactg tacgaacatc
      481 cctgacaacg cgcacggcgt gctcgctaac gccgacggta aattccagca ggtctggaac
      541 ggcgagagcg aaagttggga acccgcttcc tttacctgcg catgggtttg cgacgacaac
      601 tacacgaaga acggcgaggt ctgcgatgcg aatacgcggc gggtcgcctg cgtcaactcc
      661 ctgcccgata acgcgcattg ggccgacccg aatgccgacg gcaaattcct gcagacttgg
      721 aatggttcga cggacgaatg ggaaccgttg acctcggtgt gcgcgtgggc gtgcgacgag
      781 aatttctccc tgaatgacga agaggatggc tgtgtcgcga atacgcggcg gaccgattgc
      841 gtgagcgcga aaccggacca ctcccactac agcggtgaaa acgacgacga caagttcgaa
      901 cagacatggg atgccggaag caacgattgg acgccggcgt tcttcacctg caaatgggac
      961 tgcgacgaca attatacttt tgattcgatg gggcccacct gtgtccccga tacccggcct
     1021 gaagagtgta cgaatacctt gcccgacaat gcgtactgga tcgatccgaa cgccgatggt
     1081 acctttgagc aaacatggga tggcgacagt tgggagccgg cgaccttctc cgactgtgca
     1141 tgggagtgcg atgaacacta cacccaaaat ggccttgtct gcgatcccaa aacgcaacgg
     1201 gccgattgcg tcaaccaaat accggatcat gccttttaca gcggcgcgaa cgacgacggc
     1261 aagttcgaac agacatggga cggcgggatc gaggactgga ccccggcgcc cgaaacggtc
     1321 acctgcgagt gggaatgcga taagggatac ccgatcaatg aggcggaaga cgactgcgta
     1381 ttccagccga tcgtgtatgt caaccatgcc gcgaccgacg gcgagaacac cgggtattcg
     1441 tgggccgatg cgttcaccga cctgcaggac gccctgaaac aggtcatcgc cggtcaggag
     1501 atatgggtcg ccacaggcac ctataaaccg tcccgttgtc cctacttcga cggctgcgac
     1561 gataaggaaa aaacctttat gatgttccgt gatacaggcg tcgatttcgt tctgtacggc
     1621 ggctttgacg gtaccgagac gacccgcgat caacgcgatt gggagacgaa cgagaccatt
     1681 ctgtcgggcg atattaccgg cgacgatgcc tgggatgatg tcaacggcgg ctggctgaac
     1741 cgtaccgaca atgtctatcg cgtactggtg ttcgatcccg ccatgccttc aaacacgcta
     1801 ctcttggacg ggttcaccgt cagcggtgga catagcgaag gcatggataa tcggggtggc
     1861 gccgtttctt ccaccaacca tgccgttgag gtgcgcaact gcgttttccg tggcaatgtc
     1921 gcttcgggtg gcggcgccat atttgtcatc gatggccaac tgaccgtcga taactccttt
     1981 ttctatcgca atgcggccga tccttcttct tctggaagcg gtggagcgat agccactttg
     2041 aatgcccccc tctccctgac cgactcggtc tttgatgaga acttcgccgg cgataacgcc
     2101 ggtgcggtca actgcaatgg caatgtgacg atttccggct cttctttcct caataaccat
     2161 gccgcaggaa acgccgccgc cctttctatc gggaatgcga ctttggcaac tatcaccgac
     2221 accgattttg tcgggaatac ctctggcttc ggcggtgcca tcggcataaa taccaccact
     2281 tcggttgtcg ttgcctcctg ctatttcgag gcgaatgagg cgaccgacga aaatggaagc
     2341 ggtggagcga tcagcgttaa cgccgactcc ttctcccttt ccaattcgca gttcgttgac
     2401 aacagcgctt ccatgggttc cgcggtgaaa ctctggacgg tcgccgacgc gacggtgacc
     2461 tccgtcactt tcacgggaaa taccggttcc gcgatctccg cccaaggtgg tgcgttggtc
     2521 atcaccaatt ctaccttcac cgacaacacc gccgccgtga accgtgtttc cgccatcgag
     2581 atgcaggatg cctcctccct ttccataagc agttcgtcgt tcctgtcgaa tgaggcggac
     2641 agaagcacca ttaatgtcag agacaccctc cttttctccg taaatgactg ttccttccag
     2701 aataacaatg gatcggcaat aaatatggag ggaactcctc tacagatgac cgactgtgat
     2761 ttcatcggca accatgccgc ttccggagcc gccgtcaacc ccggcgatgg cgatcacacg
     2821 ataaccaact gccgtttcaa gaacaatgcc gccgacctgc cgggagagcc ggatgacatt
     2881 ttcgccggct ggggcggcgt catggccatg atgggggcga acatgacggt catcaactcg
     2941 gtcttcgagg gaaacaccgc ggccgaatcg ggcggcgccg tcgcagccat ctggtccacg
     3001 ctttccttta ccaactgcac ttttaccggc aacaccgacc ttaacggcga cggcatcgtc
     3061 tacatcggaa cgaacggtcc tgtcaacaac accatacttt gggacacggt ggaagaggtc
     3121 accactctca cggtcggaga ggatgtgtac gacatgggac cgagtaaccc gaccttcagt
     3181 tacagcgatg tctatggaag cggcggctca ggcaacgatt gtggtccca
//
LOCUS       DAOQUE010000335         3220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_34534_length_3220_cov_4.109953, whole genome shotgun sequence.
ACCESSION   DAOQUE010000335 DAOQUE010000000
VERSION     DAOQUE010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34534_length_3220_cov_4.109953"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKH10_09830"
     CDS             complement(<1..660)
                     /locus_tag="PKH10_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28464.1"
                     /translation="MNIYAWIILGYFALVVLIGLFTKKAAAKSASDYLVAGRNLGVIV
                     CAVVVAAEWLGGMSTIGVSEKAFKTATLQPIFYNIATAIGMVIIGFTVAAHYRRKGVH
                     TVSEMIETLFGPRARSVSAIAFLIAYITLAYVQLQTCAGVMAPLFDISWNWAVILSAA
                     IIMLYTYVGGMHALAVTGIVYLVTMYVGLGTAFFIGLYKVGGFDGLHTLLVAKGAPVN
                     LY"
     gene            complement(682..1593)
                     /locus_tag="PKH10_09835"
     CDS             complement(682..1593)
                     /locus_tag="PKH10_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNT28465.1"
                     /translation="MAITNYTELKKTVGALPPVTVAVAAAADPEVIGGLKLAHDMGFV
                     GTCIVTGDPKAIERCIADAHDDIGKYQIIAAKNDAEAGVLAVQAVREQGAKILVKGSL
                     KSELYLKAILDKERGIKASPVLSNISLFEMPSYHKFLGVTDNAIIVAPTIEEKKAIIE
                     NTRPLWKALGVDKAKVALLAAVETVSPKQPATQDAAALVVMAMRGQIKDFIIDGPLGY
                     DAAISAEAAAVKGIKNSPVCGDPDLIVAPNLETANSLGKSYKFHGSATWGGLVLGAKV
                     PAVLNSRSDDEQNRLNALLLARAMAAL"
     gene            complement(1656..2861)
                     /gene="buk"
                     /locus_tag="PKH10_09840"
     CDS             complement(1656..2861)
                     /gene="buk"
                     /locus_tag="PKH10_09840"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNT28466.1"
                     /translation="MEWRDTRVPHGSIPTEAKDSAMTATDQIVVVNTGSTSTKCSIHR
                     IDPGGPVEVSADCIRHPDDLVHRFPTIADQLDHREELVRAFLDKHLPERKCRAMGAIG
                     GMLPPVPSGVIEMNDELAHFSHKTPVYQHASNLAAPLVHRLSKDYGCPTYAVDPVGVD
                     EMAPIARISGAPDFPRFSFVHALNIRATVRKLAAQLGKKFEDMRCVACHLGGGYSIAP
                     FDRGRIVDSDNRMEGAPFTPERAGGIPPIPLLEACFSGKYTKQELHKKLYGAGGIYGY
                     LGTKDIKEVVRRIKEGDAFAKFIYDAMIYQICKEIGAMASVLDFDMDGIIVTGGVANE
                     KYLAAEITRKCERLGRIYIFPGENENEAIAGGVWRVLTGAEKAMRWPDCIMKDAEIDP
                     LYEWRKTHP"
     gene            2971..3111
                     /gene="cutA"
                     /locus_tag="PKH10_09845"
     CDS             2971..3111
                     /gene="cutA"
                     /locus_tag="PKH10_09845"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HNT28467.1"
                     /translation="MHTGHIVVLTTFDKREDAERVARILVERRLAACVQMIAPMSAPK
                     TT"
     gene            complement(3072..3220)
                     /locus_tag="PKH10_09850"
                     /pseudo
     CDS             complement(3072..3220)
                     /locus_tag="PKH10_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
BASE COUNT          586 a         1032 c          966 g          636 t
ORIGIN      
        1 gtacagattg accggcgcgc ctttggccac cagcagggta tgcagtccgt cgaaaccacc
       61 caccttatag aggccgataa agaaggcggt gccgagcccg acatacatcg tcacgagata
      121 gacgataccg gtgacggcga gcgcgtgcat cccgccgaca taggtgtaga gcattatgat
      181 cgccgccgac aggatgaccg cccagttcca actgatgtcg aacagcggcg ccatcacccc
      241 ggcgcaggtc tgcaactgga cataggcaag cgtgatgtag gcgatgagaa aggcgatcgc
      301 cgacaccgac cgggcgcgcg gcccgaaaag cgtctcgatc atctcgctga ccgtgtggac
      361 ccctttgcgg cggtagtggg ccgccaccgt gaagccgatg atgaccatgc cgatcgcggt
      421 cgcgatgttg tagaagatgg gctggagcgt cgcggtcttg aaggccttct cgctcacccc
      481 gatagtgctc atgccgccca gccattcggc ggcgaccacc accgcgcaga cgatgacgcc
      541 gagattgcgc cccgccacca gataatcaga ggccgacttg gcggccgcct tcttggtgaa
      601 caggccgatc aggaccacca gcgcgaagta accgaggatg atccacgcat agatgttcat
      661 gggaactcct tctttcagat attagagtgc ggccatggcg cgagcgagca ggagggcgtt
      721 gagccggttc tgttcgtcgt ccgaccgcga attgagcacc gccgggacct tcgctccgag
      781 gacgagcccg ccccaggtcg cgctaccgtg gaatttgtaa ctcttgccga gcgaattggc
      841 cgtctcgagg ttcggcgcga cgatcagatc gggatcgccg cagaccggcg agttcttgat
      901 acccttcacc gccgccgctt ccgcactgat ggccgcgtcg tagccgagcg gcccgtcgat
      961 gatgaaatcc tttatctggc cgcgcatcgc catcaccacg agcgccgccg cgtcctgcgt
     1021 cgccggctgt ttggggctca ccgtctcgac cgccgcgagg agcgccacct tcgctttatc
     1081 gacgccgagc gccttccaga gcggccgcgt gttctcgatg atcgccttct tctcttcgat
     1141 ggtcggggcg acgatgatgg cgttgtcggt cacgccgagg aacttgtggt aggagggcat
     1201 ctcgaacagg ctgatgttcg agagcaccgg agatgctttg atgccgcgct ccttgtcgag
     1261 tatcgccttc aggtagagtt ccgacttgag cgaacccttg accagtatct tcgcaccctg
     1321 ctcgcgcacc gcctgcaccg caagcacccc cgcttcggcg tcgttcttgg ccgcgatgat
     1381 ctggtatttc ccgatgtcgt cgtgcgcgtc ggcgatgcag cgttcgatcg ccttcggatc
     1441 ccccgtcacg atgcaggtac ccacaaagcc catgtcatgg gcgagcttga ggccgccgat
     1501 cacctcggga tcggccgccg ccgccaccgc gaccgtcacc gggggcaggg ccccgaccgt
     1561 ctttttgagt tccgtgtagt tcgtgatcgc catggtgttc tccgttatca gtcattccgc
     1621 cctttccggg cacccctctc ttgccaagag aggggttagg ggtgagtttt tctccattcg
     1681 tacagcgggt ctatctcggc gtccttcatg atgcagtcgg gccagcgcat cgccttttcg
     1741 gcgcccgtca ggacgcgcca gacgcccccg gcgatcgcct cgttctcgtt ctcgccgggg
     1801 aaaatgtata tcctgccgag ccgttcgcac ttgcgcgtta tctcggcggc gagatacttc
     1861 tcgttggcca ccccgccggt cacgatgatg ccgtccatgt cgaaatcgag cacgctcgcc
     1921 atcgcgccga tctccttgca tatctgatag atcatcgcgt cgtatatgaa cttggcgaac
     1981 gcatcgccct ccttgatgcg gcggaccacc tctttgatgt ccttggtgcc gagatagccg
     2041 tagatgccgc ccgcgccgta gagtttcttg tgcagttcct gcttggtgta cttgcccgaa
     2101 aaacaggcct ccagtagcgg tatcggcggt atgccgccgg cccgttccgg cgtgaagggg
     2161 gccccctcca tccggttgtc gctatcgacg atgcggcccc tgtcgaaagg cgctatcgaa
     2221 tagccgccgc cgaggtgaca cgcgacacag cgcatatctt cgaacttctt tcccagttgc
     2281 gccgccagtt tccgtaccgt cgcccggatg ttaagcgcat gaacgaacga gaagcgggga
     2341 aaatcgggcg cgcccgatat ccgggctatc ggtgccatct cgtccacccc gaccggatcg
     2401 accgcatagg tgggacagcc gtagtcttta gaaagacggt gcaccagcgg cgcggcgagg
     2461 ttcgacgcgt gctgatagac cggcgtctta tggctgaaat gggccagttc gtcgttcatc
     2521 tcgatgacgc cgctcgggac cggtggtagc atcccgccga tggcccccat ggcgcggcat
     2581 ttccgttcgg gcaggtgctt gtcgagaaag gcgcgcacca gttcctcgcg gtggtccaac
     2641 tgatcggcga tggtcgggaa ccggtgaacc agatcatcgg gatggcggat gcagtcggcg
     2701 ctcacctcga ccggcccgcc ggggtcgatg cggtggatgg agcacttggt ggaggtcgaa
     2761 ccggtgttga cgacgacgat ctggtcagtg gcggtcatgg cgctgtcctt cgcttcggtt
     2821 ggtatcgaac cgtgcggcac gcgggtgtcg cgccactcca tatatcaaaa agagggcgac
     2881 gatgtcaaat aaacccatac gctcccgtgc cattgcaaaa ttccccttcc ccccatatac
     2941 ttcgcccgaa ccacccctca ggaggccccc atgcacaccg gccacatcgt cgtactcacc
     3001 accttcgaca agcgcgagga cgccgaacgg gtcgcgcgga tcctcgtaga acgccgactc
     3061 gccgcctgcg tccagatgat cgccccgatg agcgccccga aaacgacgta gtaatcgaca
     3121 atggctggat cgatggtgcc cttgccaatc gtagatgcca ctttcaactg gaacaggaaa
     3181 taggcgagga aattgaaggc tgctgcccaa accaccgcct
//
LOCUS       DAOQUE010000336         3212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_34669_length_3212_cov_3.976243, whole genome shotgun sequence.
ACCESSION   DAOQUE010000336 DAOQUE010000000
VERSION     DAOQUE010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34669_length_3212_cov_3.976243"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..360
                     /gene="rpmI"
                     /locus_tag="PKH10_09855"
     CDS             160..360
                     /gene="rpmI"
                     /locus_tag="PKH10_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNT28468.1"
                     /translation="MAKIKTNKAAAKRFKSTGTGKIRRWHAFKSHILTKKSKKRKRHL
                     RQDAAVPADIMKQMKRLLPYGA"
     gene            376..735
                     /gene="rplT"
                     /locus_tag="PKH10_09860"
     CDS             376..735
                     /gene="rplT"
                     /locus_tag="PKH10_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNT28469.1"
                     /translation="MMRVKKGFKARRRRKEILKAARGNYSRRARTFKVARETVERGWA
                     FSYVSRKLKKRDFRALWIMRINAAARERGLSYSVLMNLLSKAKVTMDRKLLAHLAMED
                     PKAFDKIVDTAKALITA"
     gene            746..1036
                     /locus_tag="PKH10_09865"
     CDS             746..1036
                     /locus_tag="PKH10_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNT28470.1"
                     /translation="MSVTKVELSEMAYEHLKVDKKKAAEMVDLFFEELKNGLVADGDI
                     QISGFGKFTIKQKRQRKGRNPQTGDTMMIGERKVVSFHHSQVLKERLNSKKA"
     gene            1059..1136
                     /locus_tag="PKH10_09870"
     tRNA            1059..1136
                     /locus_tag="PKH10_09870"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1094..1096,aa:Pro,seq:ggg)
     gene            1152..1931
                     /gene="surE"
                     /locus_tag="PKH10_09875"
     CDS             1152..1931
                     /gene="surE"
                     /locus_tag="PKH10_09875"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNT28471.1"
                     /translation="MKDLILLTNDDGIGAPGIATLENRLAAASLSHAVSAPLHERSGC
                     GHSITLHDPLRVRMLGEGRFAVNGTPADAVTLYLDAPLGPRPKLIVSGINLGGNLARD
                     LFYSGTFAAAAEGFFHGVTSIAVSLYFGGPSAYAPSHFDIAADLFVRGILPFIEGALG
                     DEMYRSPRLLNVNIPVTALDGRTPDLRWTSLGSRDYGGQIVAGHDPRGRPYYWIGGDQ
                     QGFADLPGSDCNAMRDGAVSITPISLSLTDEQTLNRLRKKT"
     gene            1928..2548
                     /locus_tag="PKH10_09880"
     CDS             1928..2548
                     /locus_tag="PKH10_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNT28472.1"
                     /translation="MTEQNAPQQKNIVRRLYDWVLHWAETPYGTPALATLSFAESSFF
                     PVPPDVLLIALNMGKPKKSFYFALVCSVASVAGGMLGYFIGTALWHALSGFFFSYIPG
                     FTPEVFAKVQTMYNEYGFAAVLIAGFTPIPYKVFTIASGVFGLNFPLFVLASTISRSA
                     RFFIVSTLIYLFGEPIKKFIDRYFNLLASAFVVLIILGFVAIKYLF"
     gene            2570..3184
                     /locus_tag="PKH10_09885"
     CDS             2570..3184
                     /locus_tag="PKH10_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28473.1"
                     /translation="MHRIPLIILALLMCVACSNTSGTVSDADTAATYDDTAIVDEMAV
                     TDEDMMITDTNDEALASDDLNDEATDDTTEPDDMTDEVTDPDDIFVPDEDADTASNEP
                     CTVQFGVPNEKTGLTAEQCQPLCNCEGKFFQPPLYTEDDIAKLLAMVPVDPPAEVTED
                     PYDKPEEHQPVPGTVCGLFLDGAVPDGYRLETFESDVQITPFPG"
BASE COUNT          689 a         1003 c          936 g          584 t
ORIGIN      
        1 ggggccgcga agcgatgttc gtcgacaacg cgctcgcgaa catcaacttc atcatcaccg
       61 aactgggcgg cgaagaggcg ctcgcggtgg aacgggacac gataatcagg aataggcgca
      121 tgacggtcat gctgtctgcg aagaactaag gagaagacca tggcgaagat caagaccaac
      181 aaggcggcgg cgaaacggtt caaatcgacc gggaccggca agatcaggcg gtggcatgcg
      241 ttcaagagcc acatcctgac caagaaatcg aaaaaacgga aacggcatct gcggcaggac
      301 gcggcggtcc ccgccgacat catgaaacag atgaaacggc tgctccccta cggggcataa
      361 cgaaaaggag aaggcatcat gcgggtaaag aaagggttca aggcccggag acggagaaaa
      421 gagatcctga aggcggcgcg gggtaattac tcgcggcgcg cacggacctt caaggtcgcc
      481 cgggaaactg tcgagcgcgg ctgggcattc agttatgtga gccgcaaact caagaaacgc
      541 gatttccgcg cgctgtggat catgcgcatc aacgccgccg cccgcgaacg gggtctgtcg
      601 tacagcgttc tcatgaacct gctgtcgaag gccaaggtga cgatggaccg caaactcctc
      661 gcccacctcg cgatggagga tcccaaggcg ttcgacaaga tcgtggacac ggcgaaggca
      721 cttatcacgg cataggggga atatgatgag cgtgaccaag gtagaacttt cagagatggc
      781 gtacgagcac ctgaaggtgg acaagaagaa ggcggccgag atggtcgatc ttttcttcga
      841 ggaactgaag aacgggctgg ttgccgacgg cgacatccag atatcgggct tcggcaagtt
      901 caccatcaag cagaagcgcc agcgcaaggg gcgcaacccg cagaccggcg acacgatgat
      961 gatcggcgaa cggaaggtgg tatcgttcca ccacagccag gtcctcaagg agcgcctgaa
     1021 cagcaaaaag gcctgatctc cgtctttttt gaccaggtcg ggatgtagcg cagtttgggt
     1081 agcgtacttg aatggggttc aagtggtcgc tggttcgaat ccagtcatcc cgaccatttt
     1141 tttacatgaa catgaaggat ctgatacttc tcaccaacga cgacggcata ggcgcaccgg
     1201 gcatcgccac gcttgaaaac cggctcgccg cggcgtccct gtcacacgcc gtatcggccc
     1261 ccctccacga aagaagcggt tgcggtcatt cgataacgct ccatgatccg ctccgcgtga
     1321 gaatgctcgg cgaaggccgc ttcgcggtga acggcacccc ggccgacgcg gtgacgctct
     1381 atctcgacgc gccgctcgga ccgcgcccga aactcatcgt ttcgggtata aatctcggcg
     1441 gcaaccttgc acgggacctg ttctacagcg gcaccttcgc cgccgccgcg gagggctttt
     1501 tccacggcgt cacgagcatc gctgtctctc tctacttcgg tggcccgtcg gcctacgcgc
     1561 cttcccactt cgacatcgcg gccgacctgt tcgttcgcgg gatactgcct ttcatcgaag
     1621 gcgccctggg cgacgagatg taccggtcgc cccgactgct caatgtgaac ataccggtca
     1681 ccgcgcttga cgggaggacc cccgacctgc gctggacgag cctcggctca cgcgactatg
     1741 gcggccagat cgtggcggga catgacccgc gcggccgccc ttactactgg ataggcggcg
     1801 atcagcaggg tttcgcggac ctccccggca gcgactgcaa cgcgatgcgc gacggcgccg
     1861 tttcgataac cccgatatcg ctctcactca ccgacgaaca gaccctcaac cgattaagga
     1921 aaaagacatg accgagcaga atgccccgca gcaaaagaac atcgtccgcc gcctgtacga
     1981 ctgggtcctg cactgggccg agacccccta cggcaccccg gcgctcgcga cactttcctt
     2041 cgccgaatcg agtttcttcc cggtgccgcc cgatgtgctg ctcatcgcgc tcaacatggg
     2101 caaaccgaaa aagtcgttct atttcgcgct ggtctgttcg gtcgcctcgg tggcgggcgg
     2161 gatgctcggc tacttcatcg gcacggcgct gtggcacgcg ctgtcgggct tcttcttctc
     2221 ctacatcccc ggcttcaccc ccgaggtgtt cgccaaggtg cagaccatgt acaacgagta
     2281 cggtttcgcg gcggtcctca tcgccgggtt cacgccgata ccctacaagg tcttcaccat
     2341 cgcaagcggc gtgttcgggc tcaatttccc tttgttcgtc cttgcctcga cgatctcgcg
     2401 ttcggcccgt tttttcatcg tcagtacgct catctatctg ttcggcgaac cgataaagaa
     2461 attcatcgac cgctatttca acctgctggc gtcggccttc gtggtgctca tcatccttgg
     2521 cttcgtcgcc ataaaatacc tgttctagtt gatctcgcgg aggacacgca tgcatcggat
     2581 ccctctcatt atccttgccc tgctcatgtg cgtcgcctgt tcgaacacct ccggcacggt
     2641 ctcggacgcc gacaccgccg cgacctacga cgatacggcg atcgtcgatg agatggcggt
     2701 gaccgatgag gatatgatga taacggacac gaacgacgag gcgctcgcaa gcgacgacct
     2761 gaacgacgaa gcgaccgatg acaccacgga gccggacgac atgaccgacg aggtgaccga
     2821 ccccgacgat atattcgttc ccgacgagga tgccgacacc gcgtcaaacg aaccctgcac
     2881 cgttcagttc ggcgttccca acgagaagac cgggctcacc gccgaacaat gccaaccgct
     2941 ctgcaactgc gaaggtaagt tcttccagcc gccgctctat accgaggatg acatcgcgaa
     3001 actgctcgcg atggtgccgg tggacccgcc cgccgaggtg accgaggacc cctacgacaa
     3061 acccgaggaa caccagccgg tgccggggac cgtctgcggg ctcttcctcg acggcgcggt
     3121 gccggacggc taccggcttg agaccttcga gagcgatgtc cagatcacgc cttttcccgg
     3181 atagtttcga cctgatggtg ggcagcctcc ct
//
LOCUS       DAOQUE010000337         3195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_34940_length_3195_cov_3.670382, whole genome shotgun sequence.
ACCESSION   DAOQUE010000337 DAOQUE010000000
VERSION     DAOQUE010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34940_length_3195_cov_3.670382"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="PKH10_09890"
     CDS             <1..1314
                     /locus_tag="PKH10_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28474.1"
                     /translation="ALFKKTVGMEWTNSALSLACFLLLFFVTTRLSGSRVTALLTALL
                     FVLSTSVIFHAGSYFSHPAVLLAVLALVTTVMAAVTTDDRRRRDRLFLLAGAIVGIVL
                     FLRIWDAVIVAVALGLFLTRELFLPPATDRATGLAARSRSFLRHLGLIAAGAAPLILL
                     FLAYQKIYTGSFFTTPYQLFYYQPQLLTGDRIGEINRSFDAYYEKGLLTLTPNWLKAQ
                     AQWTNKYLVWLLLLLPFLKLGRLRRFEWFVVFFPIAYIAGYAIHNSGGGDSFGARYYY
                     PALWCWFYGAAETTQFVARRLWRYIHWVPYAAFLYFLCIYWQEDFPGKETGIMRGVNK
                     RFALYDHIERRVPPEEKAIVLIKRPTAMDASFFTRHELDLSDRILYGRYFVKPRLAED
                     LKQNFPDRKIYVFNWNKHTRQIEFDEIGSGPAPEADEKEVEHTGR"
     gene            1283..>1623
                     /locus_tag="PKH10_09895"
     CDS             1283..>1623
                     /locus_tag="PKH10_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT28475.1"
                     /translation="MKKKWNILVVDDDPSILRSFEKFLRDEGHAVATARDGQEAWERV
                     VATRPDIMILDLTMPRMGGLDLLEKLRRELGDRMPLVITVTAYGDLSAAVKATRLGSY
                     DFLTKPIPLEKL"
     assembly_gap    1624..1633
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1634..2633
                     /locus_tag="PKH10_09900"
     CDS             <1634..2633
                     /locus_tag="PKH10_09900"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT28476.1"
                     /translation="FNETVSLVEKEPDGVVPEDIVGRSAVMVEIYKQIATLATNRATV
                     LITGESGVGKEMVARAIHRVSADTAAPWVAVNCAAIPPTLIESELMGHLKGSFTGADH
                     ASEGKLGLIGAGTLLLDEITEIPPELQAKLLRLLQEREYFPVGASTPRRFEGRIIAIS
                     NRDLEAEVRAGRFREDLYYRLHVVHFRVPPLRDHPEDIELLVPHFIRKTNRQMQTAIE
                     GFTREALLFLTERSWPGNIRELENVIIKAAIASKDRLLTRDTIEATMPTGSPRITGTT
                     VPAAEETLSLKDIEKRHIERVYRACGHHKTKTAAALGITRPTLDKKLEEYGITKDV"
     gene            2626..>3195
                     /locus_tag="PKH10_09905"
     CDS             2626..>3195
                     /locus_tag="PKH10_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28477.1"
                     /translation="MFEPASISALIYTLLTVGLALAWRIVAAGKRTADGIFAFLFLLS
                     LALLYSVASVGYFPFFSPLFFFALILCQSAAAFYVYAFPNAIRPPTRWLAFIAASDAL
                     FLLFFLFDPAFAFPAVVLSSTHLAALSFLFTLRKQRHLRPAVRAGANRAITAIIATQL
                     LLGATTAAGIRLFMTTPFLAAVPAVILATA"
BASE COUNT          564 a         1127 c          877 g          617 t
ORIGIN      
        1 gcactcttca aaaagacggt cgggatggaa tggaccaatt ccgccctcag tctcgcctgt
       61 ttccttttgc ttttcttcgt gaccacccgc ctctcaggtt cacgcgtgac agcgcttctg
      121 accgctcttc tgttcgtgct ctcgacctcg gtcatattcc acgcgggctc ctatttctcc
      181 catccggcgg tcctgcttgc ggtgctggcg cttgtcacca ccgtcatggc ggccgtcacg
      241 acggacgacc ggcgtcggcg cgaccggctg ttcctgctcg ccggcgcgat cgtcggcatc
      301 gtccttttcc tgcggatatg ggatgccgtc atcgtggccg tcgcgctggg gctttttctt
      361 acgcgtgaac tgttcctccc cccggcgacc gaccgcgcga ccggcctcgc cgcccgttcc
      421 cggtccttcc tgcgccacct cggcctcatc gccgccggcg ccgcgccgtt gatcctgctg
      481 ttcctcgcct accagaagat atacaccggc tcctttttca ccaccccgta ccaactgttc
      541 tactaccaac cacaactcct gaccggagac cgcatcggcg agatcaaccg gtcgttcgac
      601 gcctactacg agaaaggcct gctcaccctc accccgaact ggctcaaggc acaggcacag
      661 tggaccaaca aatacctcgt ctggctcctg ctccttctgc ctttcctgaa actcggtcgc
      721 ctgcgccgct tcgaatggtt cgtcgtgttc ttccccatcg cctatatcgc cgggtacgcc
      781 atccataact ccggcggcgg cgacagtttc ggcgcccgct actactatcc ggcgctctgg
      841 tgctggttct acggcgccgc cgagacgacc cagttcgtcg cccggcgact gtggcgctat
      901 atccactggg tcccctacgc cgcgttcctc tacttcctgt gcatctactg gcaggaggat
      961 ttccccggca aggagaccgg catcatgcgc ggcgtcaaca agcggttcgc cctctacgac
     1021 catatcgaac ggcgggtccc ccccgaggag aaggccatcg tcctcatcaa gcggcccacc
     1081 gcgatggacg cgtcgttctt cacgcggcac gaattggatc tatccgaccg catcctgtac
     1141 ggtcgctact tcgtcaaacc gcgactcgcg gaggatctca agcagaactt ccccgaccgg
     1201 aagatatatg tcttcaactg gaacaagcac accagacaga tagaattcga cgagatcggg
     1261 agcggaccgg caccggaggc ggatgaaaaa gaagtggaac atactggtcg ttgacgacga
     1321 cccctcgata ctccggagtt tcgagaaatt cctgcgcgac gaggggcatg ccgtcgcgac
     1381 ggcgcgcgac gggcaggagg cgtgggaacg ggtggtcgcg acccggcccg acatcatgat
     1441 actcgatctc accatgccgc gcatgggggg gctcgacctg ctcgagaagc tgcgcaggga
     1501 gctcggcgac cggatgccac tcgtcatcac cgtcaccgcc tatggcgacc tgtcggcggc
     1561 ggtcaaggcg acccggctcg ggtcctacga tttcctcact aaaccgatac cgctcgaaaa
     1621 actnnnnnnn nnngttcaat gaaacggtat cgctcgtcga aaaggagccg gacggcgtcg
     1681 taccggagga catcgtgggc cggtcggccg tcatggtcga gatatacaag cagatagcga
     1741 cgctcgccac caaccgggcg accgtcctca tcaccggcga gagcggcgtc ggcaaggaga
     1801 tggtggcgcg cgccatccac cgcgtatcgg ccgacaccgc cgccccgtgg gtggcggtca
     1861 actgcgccgc gataccgccg acgctcatcg agtcggaact catggggcac ctcaagggaa
     1921 gtttcacggg ggccgaccat gcctccgaag ggaaactcgg gctcatcggc gctggcacgc
     1981 tcctgctcga cgagataacc gagataccgc ccgaactgca ggcgaaactc ctccggctcc
     2041 tgcaagaacg cgaatacttc ccggtcggcg cttcaacgcc gcggcgattc gagggccgca
     2101 tcatcgcgat atcgaaccgt gaccttgagg ccgaggtgcg ggcgggccgg ttccgcgaag
     2161 atctctacta ccggcttcat gtggtccatt tccgggtacc gccgctccgc gaccacccgg
     2221 aggacatcga actgctcgtt ccccatttca tccgcaagac gaaccgtcag atgcagaccg
     2281 ccatcgaggg cttcacccgc gaggcgctcc tgttcctcac ggagcggtcg tggccgggga
     2341 atatccgtga actggagaat gtcatcatca aggcggcgat agcgtccaag gaccggctcc
     2401 tgacccgcga cacgatagaa gcgacgatgc cgacggggtc cccgcgcatc accggcacca
     2461 ccgttccggc ggcagaagag acactctcgc tcaaggatat cgaaaaacga catatcgagc
     2521 gggtctaccg cgcctgcggc caccacaaga cgaagaccgc cgccgccctc ggcatcaccc
     2581 gtccgacact cgacaagaaa ctcgaagaat acggcatcac aaaagatgtt tgaaccggca
     2641 tcgatatcag cgctcatcta caccctgctc accgtggggc ttgcgctcgc atggcgcatc
     2701 gtcgcggcgg gtaaacggac cgccgacggc atattcgctt tcctgttcct cctttcgctc
     2761 gcgcttcttt acagcgtcgc ctcggtcggt tatttcccct tcttctcgcc gctttttttc
     2821 tttgcgctga tcctctgcca gtcggcagcc gccttctatg tctacgcctt tccgaacgcc
     2881 atccggcccc cgacgcgctg gctcgccttc atcgcggcga gcgatgcttt gttccttctg
     2941 ttcttcctgt tcgaccccgc cttcgccttt ccggcggtcg tgctctcgtc cacccatctc
     3001 gcggcgctct ccttcctctt cacgctccgt aaacaacgcc atctccggcc cgcggtccgc
     3061 gccggggcga accgcgcgat aacggcgatc atcgcgacac aactgctcct cggcgctacg
     3121 accgccgccg gcatccgcct gttcatgacc acaccgttcc tcgccgccgt accggccgtg
     3181 atccttgcga ccgcc
//
LOCUS       DAOQUE010000338         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_35162_length_3183_cov_4.433504, whole genome shotgun sequence.
ACCESSION   DAOQUE010000338 DAOQUE010000000
VERSION     DAOQUE010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35162_length_3183_cov_4.433504"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PKH10_09910"
     CDS             complement(<1..278)
                     /locus_tag="PKH10_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28478.1"
                     /translation="MRWIFLVSMMLTVGGGLLSAQERLTFDQCVAESLRESESLRSVE
                     ASIEAARADADSTFWSFFPTASINVSYLKLYFEPEPEGLAMPSIPGFDL"
     gene            complement(462..866)
                     /locus_tag="PKH10_09915"
     CDS             complement(462..866)
                     /locus_tag="PKH10_09915"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNT28479.1"
                     /translation="MGKLAAEYLVHVKVLDEQHQRLFDIIEELREANATGKTREVAAK
                     LLGDLCDYAHIHFKEEEDLMKKAGYAALSAHRVAHRDFISRVEKMMADLGRGHDATLG
                     MDLSAFLTEWLQKHVRIADQQYVEILHSRGIK"
     gene            complement(901..976)
                     /locus_tag="PKH10_09920"
     tRNA            complement(901..976)
                     /locus_tag="PKH10_09920"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(941..943),aa:Val,seq:cac)
     gene            1134..2999
                     /locus_tag="PKH10_09925"
     CDS             1134..2999
                     /locus_tag="PKH10_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HNT28480.1"
                     /translation="MSHELVYVVPEQSKERDALKEFILKHPYLRFVSLVGVDFLGNDT
                     DERIPIEYFLKNLEDIFAGGVQTDGSSVNLPGIAELNDAKVDFIIDPDRAWFVDYNPD
                     LLDRNGVPVGTIRIPIFFRHHERFVCSRSVLHDTLEHLKRELRSLLAADRGFLAAHGI
                     AAAGIADIYFTLGTELEFWVSSPAPQVSSRELKVTQSLKESYWKRTRGEIRTCLEESL
                     LLLQRYGLEPEMGHKEVGGVKGKITEQGRLHDVMEQLEIDWRFSDPLQAADNDILARF
                     AVKDTFMRHGLSVTFVAKPVEGIAGNGKHMHIGIGLRLADGRRVNLFDPNDPKSHLSN
                     YGYGAVMGILRNWEAVNPFVSHSNSALRRLQPGFEAPVSIVASLGRSPESPSRNRTVL
                     AGLVRGDSPLSIRFEVRAPNPHTNTYLAAAAMYLAMLDGMRYAAGRTPADLHRELAKK
                     RGEQANYLAADREYLSEEDIFEHYSEEGRTERYGKSPATVWEVMTHIENPPTLYEGTP
                     MTASVVRSFHISALNKWVIDICGKELDGIRRELAELRRYPDRENKLDEKRWGEFEALR
                     NDIIKDDERRPCAASRLSALFEKKEYAKASEALVSFRRKVSEARDAYRLYLGNAI"
BASE COUNT          677 a         1049 c          838 g          619 t
ORIGIN      
        1 agatcgaatc cgggtatcga aggcatcgca agtccctccg gctcgggctc aaaatagagt
       61 ttcagataac tcacattgat gctggccgtc gggaaaaaac tccagaaggt gctgtccgca
      121 tcggcccgcg ccgcctctat cgacgcctcg accgagcgga gcgattcgct ctcgcgcaga
      181 ctttcggcga cacactggtc aaaggtcaat cgctcctgcg ccgacagcaa acccccgcca
      241 accgtcaaca tcatactcac cagaaagatc caccgcatct cttttctccc tcacttatgc
      301 tatctgatac gattttcagt catcatcgtt cttttcagaa acaacctcga ccgttttgaa
      361 caaaaaacta aaaaggtcgg tcgttagaat aacgccacgg accgatttgt caagaggttt
      421 ctgctcgcgc gcagtatctt attgttatta aagagtattt cctactttat gccacgggaa
      481 tgcaggatct cgacatactg ttggtcggcg atcctgacat gcttctggag ccactcggtc
      541 aggaaggccg agaggtccat tcccaaggtg gcatcgtgtc cgcgccccag atcggccatc
      601 atcttctcta ccctgctgat gaagtccctg tgggcgaccc ggtgggccga cagggcagcg
      661 taccccgcct tcttcatcag atcctcttct tccttgaaat gaatatgggc atagtcgcac
      721 agatcgccaa gcagtttggc cgccacttcg cgggtcttgc cggtcgcgtt cgcctcacgc
      781 aattcttcaa tgatgtcgaa caaccgctgg tgctgttcgt caagcacctt cacatggacc
      841 agatattcag ccgctaactt ccccatcgcg atcctccttc cgctgtccag atgataaaag
      901 tggtgggcga tacaggactc gaacctgtga ctttctgcat gtgacgcaga cactctacca
      961 actgagtcaa tcgcccacac gaagcgtact tctacacaaa gagggaccga ttgtcaactc
     1021 tttttctctt aggaaccacc cttcccgatg ctaacagtga tataacatca cgaattggca
     1081 tttttctttc cgatcgctat actcggcgcg tcaacggacg gaggaggagg ccgatgtcgc
     1141 acgaactggt ctatgtggtc ccggagcagt cgaaggagcg ggacgctctc aaagaattca
     1201 ttctcaaaca tccctatctg cgcttcgttt cgctcgtcgg ggtcgatttc cttggcaacg
     1261 ataccgacga acggataccc atcgagtatt tcctgaagaa cctggaagat atcttcgcgg
     1321 gcggcgtcca gaccgacggt tcgtcggtca accttcccgg catcgccgaa ctcaacgacg
     1381 cgaaggtcga cttcatcatc gatcccgacc gcgcgtggtt cgtcgactac aaccccgatc
     1441 ttctcgaccg caacggcgta cccgtcggca cgatacggat accgatattc ttccggcacc
     1501 atgagagatt cgtctgctcg cggtcggtcc tgcacgacac gctggaacac ctgaaacgcg
     1561 aactgcggtc cctgctcgcc gccgatcgag gattcctcgc ggcgcacggc atcgccgccg
     1621 ccggcatcgc cgacatctac ttcacgctcg gtaccgaact ggagttctgg gtatcctccc
     1681 ccgccccgca ggtctcctcg cgcgaactca aggtgaccca gtcgctcaag gaatcgtact
     1741 ggaaacggac ccgcggcgag atacggacct gcctcgaaga atcgcttctg ttgctccagc
     1801 gctacgggct cgaacctgag atgggtcaca aggaggtcgg cggcgtcaag gggaaaataa
     1861 ccgagcaggg gcggctccac gatgtgatgg agcagttgga gatagactgg cgtttcagcg
     1921 accccttgca ggcggccgac aacgacatac tcgcccgatt cgccgtcaag gacaccttca
     1981 tgcggcacgg tctttcggta accttcgtcg ccaagccggt cgaggggatc gccgggaacg
     2041 gcaaacatat gcacatcggc atcggactca gactcgctga cggccgccgg gtgaacctgt
     2101 tcgacccgaa cgatccgaaa agccacctgt cgaactacgg ctacggcgcg gtgatgggaa
     2161 tcctacgcaa ttgggaggcg gtcaacccct tcgtatcgca ctccaactcg gcgctccggc
     2221 ggctccagcc cggcttcgag gcgcccgtct ccatcgtcgc ctcgctcggt cgttcccccg
     2281 aaagcccgtc acgcaaccgc acggtgctcg ccggactcgt ccgcggcgac agcccgctct
     2341 cgatacggtt cgaggtgcgc gcccccaacc cgcacaccaa cacctatctt gccgccgccg
     2401 cgatgtacct cgcgatgctc gacgggatgc gctatgccgc cgggagaaca ccggccgacc
     2461 tgcaccggga actggccaaa aaacgcgggg aacaggcgaa ctatctcgcc gcagaccgcg
     2521 aatatctttc cgaagaggac atcttcgagc actacagcga agaggggcgg acggagcggt
     2581 acggcaagag ccccgcgacc gtatgggagg tcatgaccca catcgaaaac cccccaacgc
     2641 tctatgaagg gacccccatg accgcatcgg tggtgcgctc cttccacata tcggccctca
     2701 acaaatgggt catcgacatc tgcgggaagg aactcgacgg catccgccgt gaactggccg
     2761 aactgcgccg ttaccccgat cgcgagaaca aactcgacga aaaacgctgg ggcgagttcg
     2821 aagcgctccg caacgacatc atcaaggacg acgaacgccg gccctgcgcc gcttcccgac
     2881 tctcggcgct gttcgagaag aaggaatacg ccaaagcatc cgaggcgctt gtctcgttcc
     2941 gccggaaggt ttccgaggcg cgcgacgcct accgactcta cctcggcaac gccatctgac
     3001 cctacgcccg ttccccctca cttgacaccg cgcatgtcgc gggtagagtg tctttcatga
     3061 aaggaacggc tctcggcatg ctcgcccgcc atcggctccc tatctccctc gcgggcgccg
     3121 gcctgctcac cgccgccgcc tggtacgcgg tgccctaccc gaccatggcc ccgaccctct
     3181 ccg
//
LOCUS       DAOQUE010000339         3181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_35197_length_3181_cov_8.737684, whole genome shotgun sequence.
ACCESSION   DAOQUE010000339 DAOQUE010000000
VERSION     DAOQUE010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35197_length_3181_cov_8.737684"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKH10_09930"
     CDS             <1..377
                     /locus_tag="PKH10_09930"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNT28481.1"
                     /translation="QVVITPNLRELKALASALGVTQSDPALLARDVSRVTGAAVVTTF
                     EGEKATVLVVEKGTASEEPFTLVPTKRPVHGTGCRFSTAIACGFAQKKGLLDSVKIAI
                     KKVAELVENREEMPSSGQDYLS"
     gene            452..>2234
                     /locus_tag="PKH10_09935"
     CDS             452..>2234
                     /locus_tag="PKH10_09935"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNT28482.1"
                     /translation="MKKFLGLCVAVVLLFFVVSCESDCVAGQNDEGCGIQSQAATDGE
                     IPWESAEEGTNALFGTMSGGVVAGATDLSSAVHRIEVPGEDWQVRTGDNIPPVANAGE
                     DQNVIEWQIVTLDASASYDPDNGPSTPVTYQWEQTGGTAVTLNNPTTAHPTFKAPLVP
                     PIGETLSFRVTVRDGDPEFSPSDIVAINVNLRPTQTVGFTETWESGDFAAYWVPESST
                     QYGQNLIADNTYLPTHGGTHHLIQNSTSGYARNEVILFANLNALDYVYLDFWHKRYNN
                     DSMHNITTSPFSYSQNYDGVSISNNGINWYRVTPNPWTAYTSYTKNSYRLDTISGISM
                     TSKVLIKFQWYDYYNYSSYTYGYGWDDIELYRNRAPTADAGEYQTVNEGAAVTLHGEG
                     SSDPDTGDTLTYSWVKTVGPNNITLAGADTANPTFTAPSPIWENVTYTFRLRVTDKRG
                     LYHEDTVNVTVTDNVPNTKPVVNAGEDVNAVEWEYVTLTAVASDEDGGPNATLSYSWV
                     QDSGPTVQNLTGTTTNSITFRTPVVTAGGQTAVFTVTVSDGVSADNASDSVNVVIAER
                     TRTNIGLNETWESGDFAAYWVPESSTQY"
     assembly_gap    2235..2334
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2355..>3181
                     /locus_tag="PKH10_09940"
     CDS             2355..>3181
                     /locus_tag="PKH10_09940"
                     /inference="COORDINATES: protein motif:HMM:NF014108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28483.1"
                     /translation="MTLHGEGSSDPDGETLSYTWTKTSGPNNITLTGANTVNPTFTAP
                     TPIWADVSYTFQLEVCDGSNGHCATDLVVVTVKDSIPNTVPVANAGDDKNVIDYELFT
                     LDATGSSDADGGPNALLSYTWTQTLGTPVTISNDYLGHATIRAPYTLPATPETLTFEV
                     EVCDGQGCDTDEVSVTVGDRASKSPVPDYNEGFESGAFNISWVSIPTVDGRTRVWDNF
                     TPRTGTYHVLQDSQVDNSWSLNQLVLFADLAGKTDVYVEFYHKHFNDEGHQMPASFVG
                     "
BASE COUNT          704 a          839 c          958 g          580 t
ORIGIN      
        1 ctcaggtcgt catcacgccg aatctcagag aactcaaggc ccttgcgtcg gcgctcggtg
       61 tcacacagag cgatcccgcc ctccttgcgc gagatgtcag ccgcgtgacc ggtgcggcgg
      121 tggtgaccac tttcgaagga gaaaaagcga cggtgcttgt ggtggagaaa gggacggcga
      181 gcgaagagcc gttcacgctc gtgccgacca agcggcccgt ccacggtact ggatgccgtt
      241 tctcgaccgc catcgcgtgt ggctttgctc agaaaaaagg tctgttggac tcagttaaga
      301 tcgcaataaa aaaagtggcg gaacttgttg agaatagaga ggaaatgccc agttccggac
      361 aagactatct ttcctgagat gaaataagaa aagagttgct ttttgcccct gaagggctac
      421 cattacccga gactttttag gggagttctc aatgaaaaag tttttgggtc tttgcgtggc
      481 ggtcgtgctt cttttcttcg tcgtttcgtg cgagagcgac tgcgttgctg gtcagaacga
      541 tgagggatgc ggtatccagt ctcaagcggc gaccgatggc gagatcccgt gggagagcgc
      601 cgaggagggg acgaacgccc tgttcgggac gatgtccgga ggtgtcgttg cgggagccac
      661 cgatctttcg tccgccgtgc accgcataga agttccgggg gaagactggc aagtgcgtac
      721 cggcgataac atcccgccgg tcgccaacgc cggtgaggac cagaatgtca tcgagtggca
      781 gatcgtcaca ctcgatgcct ccgcttcgta tgaccccgac aacggtccaa gcactcccgt
      841 cacctatcag tgggaacaga ccggcggcac ggcggtgacg ctgaataatc ctactaccgc
      901 ccatcccact ttcaaggcgc cgctggtgcc tccgatcggc gagacccttt ccttccgggt
      961 gaccgtccgt gacggcgacc ccgaattcag cccttccgat atcgtcgcga taaatgtgaa
     1021 tctgcggccc acccagacgg tgggcttcac cgagacatgg gaatctggcg atttcgcggc
     1081 gtactgggtg cccgagagca gcacgcaata cgggcagaac ctgatagcgg acaacaccta
     1141 tctgccaacg cacggcggga cgcatcatct tatccagaac tcgacgagcg ggtatgcgcg
     1201 gaacgaggtt atccttttcg cgaacctgaa cgcactggat tatgtatatc tggacttctg
     1261 gcacaagcgt tacaacaacg acagcatgca caacatcacg acgagtccgt tcagctacag
     1321 tcagaactac gacggggtct cgatctcgaa caacggtatc aactggtacc gcgtgacgcc
     1381 gaacccgtgg acggcgtaca cgagttatac gaagaacagt tatcgtctgg acacgataag
     1441 cggcatctcg atgacgagta aggtgctgat caagttccag tggtacgact actacaacta
     1501 cagcagttac acctacgggt acggctggga cgacatagag ttgtaccgga accgggcacc
     1561 gacggcggat gccggcgagt atcagacggt gaatgaaggg gccgcggtga cgctacacgg
     1621 agagggcagc agcgatcctg acacggggga cacgctgacc tacagctggg tgaagacggt
     1681 gggaccgaac aatatcacgc tggcgggggc tgacacggcg aacccgacct tcacagcgcc
     1741 gagtcctatc tgggagaatg tgacctatac tttccgtctg agggtgaccg acaaacgagg
     1801 cctgtaccac gaggacacgg tgaatgtgac ggtgaccgac aatgtgccga acacgaagcc
     1861 ggtagtgaac gcgggtgaag atgttaacgc ggtggaatgg gaatatgtga cgctgacggc
     1921 ggtggcgagc gatgaggacg gaggcccgaa cgcgacgctg agttacagtt gggtgcagga
     1981 tagcggcccg acggtgcaga acctgacggg gacgacgacg aacagcatca ccttccgtac
     2041 gcctgtggtg acggctggtg gccagacggc ggtattcacg gtgacggtga gcgacggggt
     2101 atccgcggac aatgcgagcg acagcgtgaa tgtggtgata gcggagagaa cgcgcacgaa
     2161 catcggtctt aacgagacct gggaatctgg cgatttcgcg gcgtactggg tgcccgagag
     2221 cagcacgcaa tacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggtga
     2341 atgaaggggc cgcggtgacg ctacacggag agggcagcag cgatcctgat ggggagaccc
     2401 tctcttatac gtggacaaag acgagcggtc cgaacaatat cacattgacc ggcgcgaaca
     2461 ccgtcaaccc gacctttaca gcgccgaccc ccatctgggc cgatgtgtcc tataccttcc
     2521 agttggaggt gtgcgacggc agtaatggac actgtgcgac cgatctggtc gtggtgacgg
     2581 tcaaggactc gataccgaat accgtgccgg tcgcgaatgc cggcgacgac aagaatgtca
     2641 tagattatga actgttcacg ctcgacgcga ccggttcgag tgatgcggat ggcggtccca
     2701 atgctctcct ttcctacaca tggacgcaga cccttggtac gccggtgacg atatccaatg
     2761 attatctcgg tcatgccacg atacgggcgc cctataccct gccggcgacc ccggagaccc
     2821 tgaccttcga ggtcgaggtg tgtgacggac aggggtgcga caccgatgag gtttcggtga
     2881 cggtcggtga ccgtgcatcg aaaagcccgg tgcccgatta caacgaagga ttcgagagtg
     2941 gcgccttcaa tatttcgtgg gtaagcatac cgaccgtcga cggccggacc cgcgtgtggg
     3001 acaatttcac gcctcgcacc gggacctacc atgtcctgca ggattctcag gtggataaca
     3061 gttggtcgct caaccaactg gtcctgttcg ccgatcttgc gggaaagacc gatgtgtatg
     3121 tcgaattcta ccacaagcat ttcaacgacg aagggcacca gatgcccgcg tcgttcgtcg
     3181 g
//
LOCUS       DAOQUE010000340         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_35234_length_3179_cov_4.736556, whole genome shotgun sequence.
ACCESSION   DAOQUE010000340 DAOQUE010000000
VERSION     DAOQUE010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35234_length_3179_cov_4.736556"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3179
                     /locus_tag="PKH10_09945"
     CDS             <1..>3179
                     /locus_tag="PKH10_09945"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNT28484.1"
                     /translation="PTWSWDTPADTVEFRYSFDETDWTATTDTSFTPANALIDGEYTL
                     YVQGRDDIGNWSDSGSFTLVVDATAPAAPTVTGTTPTNDTTPTWSWDTPADTVEFRYS
                     FTDGTGWATTTDTSFTPASALIEGPHTLYVQGSDAAGNWSASGNFAITIDTTGPIIVI
                     DPAVPCTRRTTGTMTFTVTGATTTNCRFDKPGGVFVESACTDSYAYSLDAGDGVYTLT
                     VSADDGLGNADSAEMSITLDTTPPTFQWQLPPNNTPYKAGSAVPSFVFRTKNLEAGET
                     VEIWDADANEKVAGTITVGSLCNATEDVSIPLDLPDKCGLYTLYAKVTDGVGAVTYYT
                     NNTTDEGALTSRTYTFDRSLPVIETFAIENDANGDDVLLASEDLDGIAGNGLQATIVL
                     TIEDDIDIGRTATLRNKTTGNTYTAVVAGDRTATFTGITIPEGTNSFAATLTDCGGNV
                     SDEFAKTIIVDTAPPTVLFQGPTGATPGHPLWLTAIDGVVDGSDRLTGQQLKFKITGD
                     WDGGTPVVEHTVYDYAGAEQSTYTYTAGEMTVDGTTVTIALPPLAYNKHTFEITVEDP
                     YGNARTASRTYEVDPIVPHAVIAYPTDELKLDSSKDEDGASGVQFTLGLDLTKLATPT
                     VTASTIDIVAIPITALGGVEDGTRSRKSWNGFTATLDGAGQTFPNDGSYLRLGDGFWR
                     LTVKVTDDHNNVYDTATLPLDQQIDVEVETDVACSALYLKANDKLINGRQVSPTWLNS
                     ADGDDGTYDMYVKTDAPDGGTVTLLVNGTTTYGPVNPSGGVASFTTPVALLTGGVENT
                     IVLTVYNGVATAVDTYYVRADITDPTLASSTMNPVPHVTGSLWELGYGYDDDVDHDTA
                     AILELNDVSAGRLIFTIGGIEDIAGYPNHGTVTLTAVAPIGAIGGTTTVNIDAVAPDM
                     VAEFTDLAFNDSVPGGQTDIEMKVTVTEQPSGNVYEKTIFVHVNLDRPEPVTPVVTTV
                     PARGSAFVQWTAVVGNTSAYAGAMQNRPYEYQVKYEQYGGTCSLDDNESVFDTGVAVI
                     PLADFSVADPSLNDEDFVDPKTAGEAQSY"
BASE COUNT          648 a         1058 c          948 g          525 t
ORIGIN      
        1 cgccgacctg gtcgtgggac acgccggcgg acaccgtgga attccgttac agttttgacg
       61 agaccgattg gacggcgacg accgatacct ccttcacgcc ggccaatgcg ctgatagacg
      121 gtgagtacac cctgtatgtg cagggccgcg acgatatcgg caactggtcc gatagcggct
      181 cgttcacgct tgtcgtggac gccactgccc cggcggcgcc gacggtgacc ggcacgacgc
      241 cgacgaacga tacgacgccg acctggtcgt gggacacgcc ggcggacacc gtggaattcc
      301 gttacagttt caccgacggg accggctggg cgacgacgac cgatacctcc ttcacgccgg
      361 cgtcggcgct tatcgagggg ccccacacgc tctatgtcca gggaagcgac gccgccggta
      421 actggtcggc gagcggcaat ttcgcaatca cgatagacac gaccggtccc atcatcgtca
      481 tcgacccggc ggtgccctgc acccgccgga cgaccggcac gatgaccttc acggtgaccg
      541 gcgcgacgac gacgaactgc cgtttcgaca aaccgggcgg ggtcttcgta gagagcgcct
      601 gcaccgactc atatgcctac tcgcttgatg ccggcgacgg tgtctatacg ctcaccgtga
      661 gcgccgacga tggacttggt aatgccgata gcgccgagat gtcgataacg cttgacacta
      721 cgccccccac cttccagtgg cagttgccgc cgaacaacac cccctacaag gcggggtccg
      781 cagtgccgag tttcgtgttc cggacaaaga acctcgaggc gggcgaaacg gtcgagatct
      841 gggatgccga cgcgaacgag aaggtcgccg ggacgatcac cgtcgggtcg ctctgcaacg
      901 cgaccgagga tgtctcgata ccgctcgacc tgcccgacaa atgcgggctc tacacactgt
      961 atgccaaggt gaccgatggc gtcggcgccg tcacctatta cacgaacaat acgaccgacg
     1021 aaggggcact cacaagccgc acctacacct tcgaccgttc gcttccggtc attgagacct
     1081 ttgccatcga gaacgacgcg aacggcgacg atgtcctgct cgcttcggag gatctcgacg
     1141 ggatagcggg caacggcctg caggcgacca tcgtgctgac catagaggac gacatagaca
     1201 tcggtcgcac cgcgacgctc cgcaacaaga cgaccggcaa cacctacacc gccgtcgtcg
     1261 cgggcgaccg gaccgccacc ttcacgggta tcacgatacc ggaagggacc aactcctttg
     1321 ctgcgacgct cactgactgc ggtggcaatg tctcggacga gttcgccaag acgatcatcg
     1381 tcgatacggc gccgccgacg gtgctgttcc agggtccgac cggcgcgacg ccggggcatc
     1441 cgctctggct caccgccatc gacggggtgg tggacggcag cgaccgattg accggccagc
     1501 aactgaagtt caagataacg ggtgactggg acggcgggac cccggtggtc gaacacaccg
     1561 tgtacgatta tgccggcgcc gagcagtcga cctataccta cacggcgggc gaaatgaccg
     1621 tggacggcac caccgtgacc atcgccctgc cgccgctcgc ctacaacaaa cacaccttcg
     1681 agataacggt cgaggatccc tacggcaacg cgcggaccgc ctcccgcacc tacgaggtcg
     1741 atccgatcgt cccgcacgcc gtcatcgcct atccgaccga cgaactcaag cttgattcgt
     1801 cgaaagacga ggatggcgcg tcgggcgtcc agttcacgct cggactcgac cttaccaaac
     1861 tcgccacgcc gaccgtcacc gcctcgacca tcgatatcgt cgcgataccg ataacggcgc
     1921 tcggcggtgt agaggacggt acgcggagcc gcaagtcatg gaacggtttt accgccacgc
     1981 tcgacggcgc gggccagacc ttcccgaatg acgggtcgta cctgcggctt ggcgacgggt
     2041 tctggcgtct taccgtcaag gtgaccgacg atcacaacaa tgtctatgac accgcgaccc
     2101 ttccgctcga ccagcagata gatgtggagg tcgagaccga tgtcgcctgt tcggcgctct
     2161 atcttaaggc gaacgataag ctcatcaacg gccgacaggt gtcgccgacc tggctgaaca
     2221 gcgccgatgg cgacgacggg acttatgata tgtatgtgaa gaccgacgcg cccgatggtg
     2281 gtacagtgac gcttctggtc aacggcacga cgacctacgg cccggtgaat ccctccggcg
     2341 gcgttgcctc gttcacgacg ccggtcgccc tgcttaccgg cggcgttgag aacaccatcg
     2401 tgttgaccgt ctataacggc gtcgcgaccg cggtcgacac ctactatgtc cgcgccgata
     2461 tcaccgatcc gacgctcgcc tcgtccacca tgaacccggt accccatgtc accggatcgc
     2521 tgtgggaact cggatacggc tacgatgacg atgtcgatca cgacaccgcc gcgatactcg
     2581 aactcaacga tgtctctgcg ggccggttga tcttcaccat cggcggcatc gaggacatcg
     2641 cgggctatcc gaaccacggc acggtaacgc tcacggcggt cgcaccgatc ggcgcgatag
     2701 gcgggacgac caccgtcaat atcgacgctg tagcccccga catggtcgcc gagttcaccg
     2761 atctcgcctt caatgacagc gttcccggcg gccagaccga catcgagatg aaggtaacgg
     2821 tcaccgaaca accgtccggc aatgtctacg aaaaaacgat attcgtgcat gtcaacctcg
     2881 accgtcccga accggtgacg ccggtcgtga cgaccgttcc tgcccgcggt tccgccttcg
     2941 tgcagtggac cgcggtcgtc ggcaacacct cggcctacgc cggagcgatg cagaaccggc
     3001 cgtacgagta tcaggtcaaa tacgagcagt acggcggcac ctgttcgctc gacgacaacg
     3061 agagcgtgtt cgacaccggc gtcgcggtca tcccgctcgc cgacttctcg gtggccgacc
     3121 cgtcgctgaa tgacgaggat ttcgtcgatc ccaagacggc gggcgaggcg cagagttac
//
LOCUS       DAOQUE010000341         3173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_35322_length_3173_cov_3.979795, whole genome shotgun sequence.
ACCESSION   DAOQUE010000341 DAOQUE010000000
VERSION     DAOQUE010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35322_length_3173_cov_3.979795"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PKH10_09950"
     CDS             complement(<1..1022)
                     /locus_tag="PKH10_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28485.1"
                     /translation="MTPLPSRRTSLLKILVPLVIVALVIVAALLFRHEPSFSLTPGTE
                     YRYRFSLRSEEFIAGGEGSLLSSAGIGDITTATDLEAHLLLRCVQRTDTGYLVAFSLT
                     DPDRHDILLNDRPLIPDRLSFDALFAGQTAYAQMTADGSIGELLFSPAAPPTFKRLMK
                     NILGEIQLSLRPESDWTAREHNRRGDYAARYRVERPLFSGAVTIDKQWDSYRALAPVK
                     TAMGDAKVSASFSLVPAADGYCRSLQGTEETAAYDPDGGELYRYNGEVSLSFLRAEPF
                     PAELPSLAKLLETHERSPFSAPVRDRAAEKENLRKMADDLSFDELESLLLKVSAGELS
                     GKEEITT"
     gene            complement(1019..1711)
                     /locus_tag="PKH10_09955"
     CDS             complement(1019..1711)
                     /locus_tag="PKH10_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNT28486.1"
                     /translation="MTLALLVSCENDVAFLDKKLEACIRNNLNDEDDPLTQDNLDQIT
                     LLYCGIGANKEESGINSLNGIEHLGNLEKLSLSYSHISDISPLRDLKKLKELGIGTDK
                     KITSYAPILLLPLTNLGVEGAEIEDISFLSYIPSLERINFEHNDITDISVIKQLPSLT
                     SIDISYNKVSSLEAIANHPNIQNFSFISIGYNCMDCALEEEHVAKVEEKNPDVYISCN
                     SSNQLLPENCEE"
     gene            complement(1745..2896)
                     /locus_tag="PKH10_09960"
     CDS             complement(1745..2896)
                     /locus_tag="PKH10_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28487.1"
                     /translation="MLNAKAASELAGLASGVDDSPSGMTETLQEDTNAQDPSWEKNQT
                     ELFELVGYLQLNPNDNGVPIPDQTRVEGDNVKMFFENMQKDSKSKLYHMHVYVAGKYL
                     LYSDPDDSAIAKLGSDLQNKTFTPAEEHDAPPPSEEDDTHLADGGKTWYDEHAYDWYF
                     SKRVAEYDLFGVLLTLDAGIFLNYGAQMKIRLGDDRAFTTINIDSSSSFSLPLGQYAR
                     GKLELVPYVDVSAFVEGSASFGILFASVSGTLGIQLNILTAELPLLARYEVGMAEIKT
                     KEEKCKEKDFFSPNCYEGFIREFDDLNLIKPYLDMNASMSVSLDILKHSEIYAQADLA
                     VLGITVVDTGKMTLFKDIIPGQMHYEYKVLELPQQFRRIDLEDVSQFIR"
     gene            2912..>3173
                     /locus_tag="PKH10_09965"
     CDS             2912..>3173
                     /locus_tag="PKH10_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28488.1"
                     /translation="MDAILDVVAVSKAIDTDLRGGEFKNVAAFVLDNMTHVFKKATGV
                     AQVMAQSGQSNSGASSKAAQMNVLRTILASVQMDAAKYARVSE"
BASE COUNT          705 a          803 c          898 g          767 t
ORIGIN      
        1 gtcgttatct cctccttgcc gctcagttcg cccgcgctca ccttgagcag gagcgactcc
       61 aactcgtcga acgagaggtc gtcggccatc ttccgcagat tctccttttc ggccgcgcgg
      121 tcgcggaccg gcgccgagaa cggggagcgc tcatgggtct cgagcagttt cgcgagcgac
      181 gggagttcgg ccgggaatgg ttcggcgcgc agaaaggaga gcgacacctc gccgttgtag
      241 cggtacagtt cgccgccgtc gggatcgtag gcggccgtct cttcggtccc ctgtagcgaa
      301 cggcaataac cgtcggccgc cggtacgagc gagaaggagg ccgacacttt ggcgtcgccc
      361 atcgccgttt tcaccggcgc cagcgcgcgg tacgaatccc actgtttgtc gatggtgacc
      421 gcaccggaga acagcggtcg ttcgacccgg taacgggcgg cgtagtcgcc gcgccggttg
      481 tgttcccgcg ctgtccagtc gctttcgggc cgaagcgata actgtatctc gccgaggatg
      541 ttcttcatga gccgcttgaa ggtcggcggc gcggcggggg agaagagcag ttcgccgatg
      601 gagccgtcgg cggtcatctg cgcgtaggcg gtctggccgg cgaagagagc gtcgaacgag
      661 aggcggtcgg ggatgagcgg gcgatcgttc agcaggatgt cgtgccgatc cggatcggtc
      721 agggagaagg cgacgagata ccccgtgtcg gtccgctgta cgcaccggag tagcaggtgc
      781 gcttcgaggt cggtggcggt ggtgatgtcg ccgatgccgg ccgaggagag gagcgaaccc
      841 tcgccgccgg cgatgaattc ctcactccgg agagagaaac gatagcggta ctcggtgccg
      901 ggcgtcagcg agaaggatgg ctcatgccgg aagagcagcg ccgcgacgat gacgagggcg
      961 acgatgacga gcggaacaag tatcttcaga agggaggtgc ggcgggaggg gaggggcgtc
     1021 attcctcgca gttctcgggg agaagttggt tactggaatt gcaactgata tacacatcag
     1081 ggttcttttc ttctaccttg gcaacatgtt cttcctccaa ggcacaatcc atacagttgt
     1141 agccgatcga aatgaaagag aagttttgaa tattcggatg gttcgctatg gcttcgagag
     1201 aggatacctt gttgtaactg atgtcaatac tggtcaaact aggcagttgc tttatcacgc
     1261 tgatgtcggt gatgtcgttg tgttcgaaat ttatcctctc caaagaaggg atgtatgata
     1321 aaaaactgat gtcctctatc tccgctcctt caacacccaa gttggtgagc ggcaaaagaa
     1381 gtatcggagc ataagaggtt attttcttgt cggtgcctat gccgagttct tttagttttt
     1441 taagatcccg caaaggactt atatcagaga tgtgggaata actcagactg agtttctcta
     1501 gattccctaa atgctctatg ccattaagag aatttatccc gctttcttcc ttgtttgccc
     1561 ctatgccgca gtacagtagg gttatttgat caagattatc ctgagtcaga ggatcatctt
     1621 catcattcaa attatttctg atgcaggcct ccagtttttt atcgagaaaa gcgacatcgt
     1681 tctcgcacga gaccagcagg gcaagtgtca tcgtccacca aaaccatatt cgcatggacc
     1741 gctcctaccg tatgaactgt gaaacatctt cgaggtctat tcgccggaac tgttgtggca
     1801 gttccagtac cttgtattcg taatgcatct gccccggtat gatatccttg aatagcgtca
     1861 tcttcccggt atccaccacc gttatgccca acaccgcaag atcggcttga gcatatatct
     1921 ctgaatgttt gaggatgtca agagataccg acatcgaggc attcatgtcg aggtagggtt
     1981 ttatcaaatt aagatcatca aattctcgaa tgaaaccctc ataacagtta ggggagaaga
     2041 agtctttttc tttgcatttc tcttctttgg tttttatctc cgccattccc acttcataac
     2101 gagccaacag tggcaattca gccgtcaaaa tattgagttg gatgccgagc gtaccgctta
     2161 ctgatgcgaa caagatgccg aaacttgcgc ttccttcgac aaaggcggag acatcgacat
     2221 agggaactag ttcgagcttt ccacgcgcat attgtcccag aggcaaggag aaagaagaag
     2281 aagaatctat gttgatggtg gtgaatgccc gatcatcgcc caaccggatc ttcatctgtg
     2341 caccgtaatt aagaaagatg ccggcatcga gcgtaagtag aacgccgaac aggtcatact
     2401 cggctactcg tttagagaaa taccagtcgt aggcgtgctc atcgtaccaa gtttttcctc
     2461 catccgccag atgagtatca tcttcttcgg atgggggtgg tgcgtcatgt tcttcggcgg
     2521 gcgtgaaagt cttattctgt aggtcggaac ctaatttagc gatcgcggag tcgtcggggt
     2581 cactgtacaa aagatattta cccgcgacat aaacatgcat gtggtaaagt ttactcttcg
     2641 agtctttctg catgttctcg aagaacattt ttacattgtc cccttccacc cgcgtttggt
     2701 cggggatggg gacgccatta tcgttcgggt tgagttgcaa gtaaccgacc agttcaaaaa
     2761 gttcggtctg gttcttctcc caagatggat cctgagcgtt cgtgtcttct tgcagtgtct
     2821 cggtcatacc ggaaggtgag tcgtccacgc ctgatgcgag ccccgccaac tcgctcgccg
     2881 ctttggcgtt cagcacgtac tgctgccaga cgtggacgct atcctggacg tggtggcagt
     2941 cagtaaggcg attgatacag acctgcgtgg cggcgagttc aaaaatgtag ccgcctttgt
     3001 cctggataat atgacccacg tcttcaagaa agcgaccggt gtagctcagg ttatggctca
     3061 gagtgggcaa agcaactctg gggcatcatc caaggcagcc cagatgaacg tgctgcgaac
     3121 catcctggcc tcggtccaga tggatgccgc caagtacgcc cgcgtttccg aag
//
LOCUS       DAOQUE010000342         3162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_35495_length_3162_cov_3.665594, whole genome shotgun sequence.
ACCESSION   DAOQUE010000342 DAOQUE010000000
VERSION     DAOQUE010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35495_length_3162_cov_3.665594"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="PKH10_09970"
     CDS             <1..1391
                     /locus_tag="PKH10_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNT28489.1"
                     /translation="ARRLRGEPVGRLAGLPMTIKDQFIVDGTPTSLGLAHRSKEPLYG
                     EGPLVRRLRGSDAVFLGKTNLPQLLVSYECRHALYGPANNPWDPGRAPGGSSGGEAAI
                     VAAGGSPLGLGADMGGSLRIPAHCCGVYALKPTEHRFPNDDTPLDRGFFGGLAGFEGF
                     VQQPGPLARSVDDLKLLMDVLLEKPLPGIAPVPWDSGPLHDDIKTLTVGFFTDNGLFP
                     ASPAIRTAVEEAARALEGRGARVIPFTPLDPVKGIALYLSLISADGARWMVDALVGEA
                     PIGGIGNFIRSARMPGAMRGAMRLLLRSLGQTSADFLMAHVRAADTADHWRRCAERSR
                     YRAEFRAAMENEKIDILLCPPFGLPAVPHDASYKGAGVFAASYAMLFNLLGMPAGVAP
                     VARIPDGGESDRIAGRDGVARDAIAIENGSAGLPVGVQVVGRHWREDQVLTVMKVLAE
                     VLRFHGDIGASA"
     gene            1443..2855
                     /locus_tag="PKH10_09975"
     CDS             1443..2855
                     /locus_tag="PKH10_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28490.1"
                     /translation="MKYLLTPTIAVLLLFIGCSSSSTTEQDEDTPVNDTATDETTDTV
                     ANDDDATPTDNATDTATPDEAADDILTDDGEAPDSDGGFLSGFDPTGKTCVTDIPDGR
                     IILATANDYQDDTAPWRVWVLVLADDGTLTDTGKYFETDRKLEGIGFNTNGRLAAVSS
                     WETGFVTLFALVDRTLCIAETGIVLPNLAENERVIFDQITADPRDPHRFYLTYGNPLA
                     TDEYSHYSGGIYTMTVDDTGHAELLTDHPEMHVPTAFTVLPGGTLAVALGGKEFFADE
                     LTAGPDDLAVLDLSGQTPQILQWYDVWGTPGANGSGPSVKSIGVSGNGHLLIANSNEY
                     AEDVGLIKLLSYNGETGITELTTFSDPALDSPDYLVLNGTGDTAVVMNGYFKGATLAV
                     TDEAITYVKKETHDLVQPMIRMHGEPFGNHVLISSFRTSNDESASITLAEITATGLVD
                     VSSTPVPLTGTFMIDNLAVQ"
     gene            complement(2915..>3162)
                     /locus_tag="PKH10_09980"
     CDS             complement(2915..>3162)
                     /locus_tag="PKH10_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT28491.1"
                     /translation="PDVIVLDVMMENPRAGLEFAGKMATDPAMPKIPIILLTSDGQNP
                     GWLGEAAFTWARIAKILDKPVTPEKLVDTVKSVLGKK"
BASE COUNT          566 a         1050 c         1016 g          530 t
ORIGIN      
        1 acgcgcgccg cctgcgcggt gaaccggtcg gtcgtctggc gggactcccg atgacgatca
       61 aggaccagtt catcgtcgac gggaccccta cctcgctggg actggcgcac cgttcgaagg
      121 agccgctgta cggtgaagga ccgctggtgc ggcggctgag ggggtccgac gccgtgttcc
      181 tcggcaagac caacctgccg caactgctgg tatcttacga atgccgtcac gccctctacg
      241 gaccggcgaa caacccgtgg gaccccggcc gggcgccggg cggcagtagc ggcggcgagg
      301 cggccatcgt cgcggcgggc gggtccccgc tgggactcgg cgccgacatg ggcggcagtc
      361 tgcggatccc cgctcactgc tgcggcgtgt atgccctgaa accgaccgag caccggttcc
      421 ccaatgacga tacgccgctc gaccgcggct ttttcggggg acttgccggt ttcgaaggtt
      481 tcgtacagca acccgggccg ctggcccgga gcgtcgacga cctgaaactg ttgatggatg
      541 tcctgctgga aaaacccctg ccaggcatcg ctcctgtgcc gtgggacagc ggtccgctgc
      601 acgacgacat caaaacgctg acggtcgggt tcttcaccga caacggcctg ttccccgcct
      661 ctccggcgat ccgtacggcg gtcgaggagg cggcacgggc gctcgaaggg cgcggggcgc
      721 gggtcatccc cttcacgccg ctcgacccgg tgaaagggat cgcgctgtat ctgtcgctca
      781 tcagcgccga cggcgcccgc tggatggtcg acgcccttgt gggagaagca ccgatcggcg
      841 gcatcggcaa cttcatccgt tcggcgcgga tgcccggcgc gatgcgcggc gcgatgcgac
      901 tcctgctccg ttctctcggt cagacctcgg ccgacttcct gatggcccat gtacgcgccg
      961 ccgacactgc cgatcactgg cgccggtgcg ccgaacggtc acggtaccgc gccgaattcc
     1021 gtgcggcgat ggaaaatgag aagatcgaca tccttctctg tccccccttc ggcctgccgg
     1081 cggtgccgca cgacgccagt tacaaaggtg cgggggtctt cgccgcctcc tatgccatgc
     1141 ttttcaacct gctcggcatg ccggccggtg tggcgccggt cgcccggata cccgacggcg
     1201 gggaaagcga tcgtatcgcc ggtcgcgatg gggtggcccg cgacgcgatc gcgatagaga
     1261 acgggagcgc cggactgccg gtcggggtgc aggtggtcgg tcgccactgg cgggaggatc
     1321 aggtgctcac ggtcatgaag gtgctggcgg aggtgttgcg cttccacggg gacatcgggg
     1381 cctccgcttg aattaccact gttttcgggt atgatcgctc gaccgaattc acggagggca
     1441 cgatgaagta cctgctgacg ccgaccatcg ccgtgctgtt gctgttcatc ggttgttcga
     1501 gttcgagcac cacagagcag gacgaggaca cgccggtgaa tgacaccgcg accgacgaaa
     1561 cgaccgatac cgtcgccaat gacgacgacg cgacgccgac cgacaacgcc accgacaccg
     1621 ccactcccga cgaggcggcc gacgacatcc tgaccgacga cggcgaggcg cccgacagcg
     1681 atggcgggtt cctgtcgggc ttcgacccga ccgggaagac ctgcgtcacc gacatccccg
     1741 acggccgcat catcctcgcg acggccaacg actatcagga cgataccgcg ccgtggcgcg
     1801 tctgggtgct ggtgctggcc gacgacggga cgctcaccga caccgggaag tacttcgaga
     1861 ccgaccggaa actcgaaggt atcgggttca acaccaacgg acggctcgcc gccgtatcgt
     1921 cgtgggagac cggcttcgtg acgctcttcg cgctcgtcga ccggacgctc tgcatcgccg
     1981 aaacgggcat cgtcctgccg aacctcgccg agaacgaacg ggtgatattc gaccagataa
     2041 cggccgaccc acgcgacccg caccgttttt accttaccta cggcaatccc ctcgcgaccg
     2101 acgaatattc gcactacagc ggcggcatct acacgatgac ggtcgatgac accggccacg
     2161 ccgaactgct gaccgaccat cccgagatgc atgtcccgac cgccttcacg gtgctgccgg
     2221 gcggcacgct ggcggtggcg ctcggcggca aggagttctt tgccgatgaa ttgacggcgg
     2281 gccccgacga tctggcggtg ctcgaccttt cgggtcagac accgcagata ctccagtggt
     2341 acgatgtctg gggaacaccg ggggccaacg gatcgggacc atcggtcaag tccatcggcg
     2401 tttcgggcaa cgggcacctg ctcatcgcca attcgaacga gtacgcggaa gatgtagggc
     2461 tcatcaaact cctgtcgtac aacggcgaga ccggcatcac cgaactgacc accttctccg
     2521 acccggcgct cgattcgccc gattacctcg tcctcaacgg cacgggcgac accgccgtag
     2581 tgatgaacgg ctacttcaag ggggcgacgc ttgccgtcac cgacgaggcg atcacctatg
     2641 tcaaaaagga gacgcacgat ctcgtccagc cgatgatcag gatgcacggc gaaccgttcg
     2701 ggaaccatgt gcttatcagt tcgttccgca cctcgaacga cgaatcggcg tccataaccc
     2761 tcgccgagat aacggctacg ggtctcgtcg atgtctcctc cactccggtg ccgctcaccg
     2821 gcaccttcat gatcgacaac ttggcggtac agtaagggac ccgtccctcc cccgaccgaa
     2881 aatacggggg agggacggca gagggaggga taccctactt cttgccgagc accgacttga
     2941 cggtgtcgac cagtttctcc ggggtgaccg gcttgtcgag gatcttggcg atgcgggccc
     3001 acgtgaaggc cgcttcgccg agccagccgg ggttctgccc gtcgctggtc aggaggatga
     3061 tcgggatctt gggcatggcc ggatcggtgg ccatcttgcc cgcgaattcc agacccgccc
     3121 gcgggttctc catcatgaca tcgaggacga tgacatcggg at
//
LOCUS       DAOQUE010000343         3161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_35512_length_3161_cov_3.576304, whole genome shotgun sequence.
ACCESSION   DAOQUE010000343 DAOQUE010000000
VERSION     DAOQUE010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35512_length_3161_cov_3.576304"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKH10_09985"
     CDS             <1..386
                     /locus_tag="PKH10_09985"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT28492.1"
                     /translation="GEPIPDAVFIESTVGGKVIVTDSTTGLIWAKEYASMDKDWEDAL
                     YYCESLDYAGYTDWRLPDLAELQSLVDYFRYNPSTSFPEMPVSYFWSSSSCVFGAGNA
                     WVVSFGSGSVVRPDKTIAYNARCVR"
     gene            445..936
                     /locus_tag="PKH10_09990"
     CDS             445..936
                     /locus_tag="PKH10_09990"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNT28493.1"
                     /translation="MKNAWLKLKGWAVKLATQHATPHEIAFGLAAGIFVGWLPIMGIQ
                     MAVVIVLTLPFRNINRFAAASGVWITNPVTFIPMYAFTYWVGAFFWQVDNVLSYADFT
                     AAFTGRDTLTGFFALLSEGKDFLIDIFICTWIGGAIVGVIAAIPTYYITKRAVERFRR
                     SPA"
     gene            complement(893..1111)
                     /locus_tag="PKH10_09995"
     CDS             complement(893..1111)
                     /locus_tag="PKH10_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28494.1"
                     /translation="MLVSSILIFLFSLGLAAVGWFGLADLVGQIPGAILGFCSFFVPG
                     MYFVYENMKENRTGATQGSDGNARPPVS"
     gene            complement(1176..1553)
                     /locus_tag="PKH10_10000"
     CDS             complement(1176..1553)
                     /locus_tag="PKH10_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28495.1"
                     /translation="MRTGRPLIFSLLLLHLSSLSFPAAGLLNDAYAFAAGNDRLLPSP
                     ATDENTAAIYHRETLTELNFLRSDDDADDPPSATEISDATAASSNLALRMTAPIAAFP
                     CGSASDILHVSPKHSPPLSFSPA"
     gene            complement(1574..1888)
                     /locus_tag="PKH10_10005"
     CDS             complement(1574..1888)
                     /locus_tag="PKH10_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNT28496.1"
                     /translation="MKRILAFLGVCGLMFPLWAEETVATATGGMEKGLIALGAGLAIG
                     IAAIGGALGQGRAAAAAMEGTARNPESSKRLFILLILSLALIESLVIYALVVSFSLIG
                     KL"
     gene            complement(1906..2592)
                     /gene="atpB"
                     /locus_tag="PKH10_10010"
     CDS             complement(1906..2592)
                     /gene="atpB"
                     /locus_tag="PKH10_10010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNT28497.1"
                     /translation="MHEGPFLFETLGIAERWAPLAGSLLVFVLVLLMGLAVRRDHRRR
                     DAAIPAPRPTIPAILEYLVETLVSFLGSILGEYTHEFARLLTGIFFFILCANLLGLIP
                     GLLAPTTSLSINLAMALVVFIYYHYIGIRDHGVSYIKLFLGPVWWIAFLILPIEIISH
                     FARILSLTLRLTGNMSGEHLVMGVFSHLLPIGLPAVFLFMGLFTAILQAFVFMTLSAI
                     YIFLSIEIDH"
     gene            complement(2592..2819)
                     /locus_tag="PKH10_10015"
     CDS             complement(2592..2819)
                     /locus_tag="PKH10_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28498.1"
                     /translation="MQGNWRALFYLVLAFEIPLLTVLGYFVGSQIDAMLGIADTHVVA
                     GVGIVAGLYLCWETFTRLRRFVEQSEGPGEE"
     gene            2974..>3161
                     /locus_tag="PKH10_10020"
     CDS             2974..>3161
                     /locus_tag="PKH10_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28499.1"
                     /translation="MSRWLGDDAATMNDRRTGAINNLDKLFLIAIGVIGAVLYMVKLN
                     YLLVDPWESHYSQVTWET"
BASE COUNT          702 a          859 c         1001 g          599 t
ORIGIN      
        1 ggggagaacc aattcccgat gctgttttca tcgaatcgac cgtcggcggc aaggtgatag
       61 ttacggactc gacaacaggt cttatctggg caaaagaata tgcttcaatg gacaaagact
      121 gggaagatgc cctgtattac tgcgagtccc ttgattatgc cggatatacc gattggcgac
      181 ttcctgatct tgcggaatta caatctctcg ttgactactt cagatacaat ccctcgacca
      241 gcttccccga aatgcctgta tcctattttt ggtcatcttc ttcctgtgtt tttggtgcag
      301 gtaatgcgtg ggtcgtctct tttggtagcg gaagcgtggt ccgacccgat aaaacgatag
      361 cttacaatgc ccgctgcgtc cgctgatccg ataaatttgc ctttcattag cgcgttgcta
      421 tgatgccccc gacacggagg tttcatgaag aacgcatggc tgaaactcaa agggtgggcg
      481 gtgaaactcg cgacacagca cgccaccccg catgagatcg cgttcgggct cgcggcgggc
      541 atcttcgtcg gctggctccc catcatgggc atccagatgg cggtggtcat cgtgctgacc
      601 ctgccgttcc gcaacatcaa ccggttcgcc gccgcatcgg gcgtctggat aaccaacccg
      661 gtcaccttca tcccgatgta cgccttcacc tactgggtgg gcgcgttctt ctggcaggtc
      721 gacaatgtcc tttcctacgc cgatttcacc gccgccttca cggggcgtga cacgctgacc
      781 ggcttttttg ccctgttgag cgaggggaag gatttcctca tcgatatctt catctgcacc
      841 tggatagggg gagcgatcgt cggcgtgata gcggcgatac cgacctatta catcacgaaa
      901 cgggcggtcg agcgtttccg tcgctcccct gcgtagcccc cgtcctgttc tccttcatat
      961 tctcgtagac gaagtacatg ccggggacga aaaaggagca gaagccgaga atggctcccg
     1021 gtatctggcc cacgaggtcc gcgaggccga accagccgac cgccgcgagg cccagtgaaa
     1081 agaggaatat cagaatgctc gatactaaca tgttctttct ctccatcgat aaaagttgtc
     1141 gaggcccgca acaaaaaacc ttttgatgcg gggaactatg cgggagagaa agagagaggc
     1201 ggagaatgtt tgggagagac atgcaggatg tccgatgcgc tgccgcaagg gaaggcggcg
     1261 atgggagcgg tcatccggag ggcaaggttc gacgaagcgg ccgtcgcatc ggagatttcc
     1321 gtggccgacg ggggatcgtc ggcatcatcg tccgagcgaa ggaagttcag ttccgtcagg
     1381 gtctcgcggt ggtatatcgc cgccgtgttc tcgtcggtcg ccggtgaggg caacaggcga
     1441 tcgttgccgg cggcgaaggc gtacgcatcg ttcaggagac cggcggccgg aaaggaaagg
     1501 gaggacaggt gcaggagcag aagagaaaaa atgaggggcc ggccggtcct catgcgggtc
     1561 gtcttccccg tgcctacagt ttcccgatga gcgagaacga aacgacgagc gcgtaaatga
     1621 cgagcgactc gatgagagcg agcgagagga tgaggaggat gaagagccgt ttgctcgatt
     1681 cggggttgcg cgcggtgcct tccatggccg ccgccgccgc ccgaccctga ccgagcgcgc
     1741 cgccgatcgc cgcgatgccg atggcgagcc cggcgccgag ggcgatgagc cccttttcca
     1801 tgccgccagt cgcagtggcg accgtctctt cggcccagag cgggaacatc aggccgcata
     1861 cgccgagaaa cgcaaggatc cttttcatga caacctccaa aagtgtcagt ggtctatctc
     1921 tatcgaaaga aaaatgtata tcgccgacag cgtcatgaac acgaaggcct gcaggatggc
     1981 ggtgaagagt cccataaaga gaaagaccgc cggcagaccg atcggcagga gatggctgaa
     2041 cacccccatg acgaggtgct cgccgctcat gttgccggtg agacggagcg taagcgaaag
     2101 gatgcgggcg aagtgtgaaa tgatctcgat gggcagtatg agaaaggcga tccaccagac
     2161 cgggccgagg aacagtttga tgtaactgac gccgtggtcg cgtatgccga tgtagtggta
     2221 gtaaatgaac acgacgagcg ccatggcgag gttgatggag agtgaggtgg tgggggcgag
     2281 gagcccgggg atgagcccga gcaggttggc gcacaggata aagaagaaga tgccggtgag
     2341 gagtcgcgcg aactcatggg tgtattctcc gaggatggag ccgaggaacg acacgagggt
     2401 ctcgaccaga tattccaata tcgccgggat ggtcggtcgg ggggcgggga tggcggcgtc
     2461 gcgccgccga tggtcgcggc ggaccgcaag gcccatcagc aggacgagaa caaagacgag
     2521 gagtgagccg gcaagcggcg cccaccgttc ggctatgccg agcgtttcga acaggaaggg
     2581 gccttcgtgc atcactcctc cccgggtccc tcggactgtt cgacgaagcg gcgcaggcgc
     2641 gtgaaggtct cccagcagag atagaggccc gcgacgatgc cgacgccggc gaccacatgg
     2701 gtatcggcga tgccgagcat cgcgtctatc tgcgagccga cgaagtaccc gagcacggtc
     2761 aggagcggta tctcgaacgc gagcaccaga taaaaaagag cgcgccagtt accctgcatg
     2821 gactcctcct cggtcacggg atgtgcgagg catcataccg ggagtgccgg caaaagtaaa
     2881 gaacttgact tatcgcgcgg gaacaatacc ttttcggcga ggttttttgt gtatcagggt
     2941 acgcagtcaa ctcgggagcg ttacatgcaa ttcttgagca ggtggcttgg cgatgacgcc
     3001 gcgaccatga acgaccgccg caccggcgcg atcaacaatc tcgataaact cttcctcatc
     3061 gccatcgggg tgatcggcgc cgtcctgtac atggtgaaac tcaactacct gctggtcgac
     3121 ccgtgggaaa gccactattc gcaggtcacc tgggaaacga t
//
LOCUS       DAOQUE010000344         3156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_35592_length_3156_cov_4.555305, whole genome shotgun sequence.
ACCESSION   DAOQUE010000344 DAOQUE010000000
VERSION     DAOQUE010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35592_length_3156_cov_4.555305"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..384
                     /locus_tag="PKH10_10025"
     CDS             19..384
                     /locus_tag="PKH10_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT28500.1"
                     /translation="MTEIHAKRTSAAVYRFVTIPGVMATVSPLLGLLGTVFGMIKIFT
                     RFSDMGGNPLILAGGIWEALLTTAAGLSVAIPSLIIYRYVLYRADRAAGEIEFAAERL
                     MAWLDERQRNTDNREGSPA"
     gene            381..1523
                     /locus_tag="PKH10_10030"
     CDS             381..1523
                     /locus_tag="PKH10_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT28501.1"
                     /translation="MKTRVSLLIAALAVAVCAACSSAQPASETKADEGTSATAEATAA
                     KPNAGAAKTQDEIDEFVSAAREGDLNAVKTFYNTGGDINKPNARGQTALMWAAQRGHL
                     EVVKFLVQLGANVNAVDADRQEPVLMWATKKGHKEVVEFLIASGADVNQGDQFKETPL
                     MDAARYGHKDLCELLLNKRARVNDTDVNGESALMWAAANGVAEVVKFLIGYGADINRI
                     NYKTGETALGKAKNKGFNDIVAMMQRQAQVEGNLIKRPPRPEDKDYVNEPPDEKELAR
                     YWGGRESALPEMLDSKIIGIHMNSFIPNITQCYQERIQQGDKNLMGQMWLKVRVAGSG
                     KIIDIFFETEKYQSSLFGDCILDAIRKKDFPMFYQGLMEFKYSYTL"
     gene            1619..2020
                     /locus_tag="PKH10_10035"
     CDS             1619..2020
                     /locus_tag="PKH10_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28502.1"
                     /translation="MNRLLVCYFCGLFVLLGCGTVPTQKTAEDTSASKETRVRQVAER
                     FMKGNRPTDDWKDDIVKSYLEKNTPETECHMICEKDENACAEKKEGLNRPHWVVRYTP
                     ECVRDKKCEGGFFSVYIDDETERVICVWGAK"
     gene            2017..2331
                     /locus_tag="PKH10_10040"
     CDS             2017..2331
                     /locus_tag="PKH10_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28503.1"
                     /translation="MKSHLGPILGAVAGIIDVIPMLAQGLPWEADASAFCLWVIAGFF
                     IQRTDLQLPAPLKGIAISFLLLIPAAILIAAKEPFSLIPISIMTLLLGAALGYAIKKV
                     TA"
     gene            2328..2684
                     /locus_tag="PKH10_10045"
     CDS             2328..2684
                     /locus_tag="PKH10_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016900206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HNT28504.1"
                     /translation="MMLLVVKYAATALVVVVVSEFAKRSDKLGALIASLPLVTIMVMF
                     WLYAEKQGTEKVANHAYYTFWYVIPTLPMFLLMPWLLRRGTGFWAALGLSVLLTAACF
                     VLTAFIAKRFGVELIP"
     gene            complement(2695..>3156)
                     /locus_tag="PKH10_10050"
     CDS             complement(2695..>3156)
                     /locus_tag="PKH10_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNT28505.1"
                     /translation="LLAAIGRCLEKNHHAVMVVAEGAGQKFFAGDKAKDASGNVLHKD
                     IGLFLKDEMNRYFKERKLDFSIKYFDPSYLIRSVAAQGTDAIFCHHLAEHAAHAAMAG
                     KTNCLIGHWNNFFTHVPIALATIERRKIYPEEALWKGVLCSTNQNDYFQAK"
BASE COUNT          671 a          922 c          939 g          624 t
ORIGIN      
        1 tgaggagacg ccgcccgcat gaccgagatc cacgccaagc ggacgagcgc cgccgtgtac
       61 cggttcgtga cgataccggg cgtcatggcg acggtgtcgc cgcttctcgg cctgctcggc
      121 acggtgttcg gcatgataaa gatattcacc cgcttctcgg acatgggagg caacccgctg
      181 atactcgcgg ggggcatctg ggaggcgctg ctgacgaccg ccgccgggct gtcggtggcg
      241 ataccgagcc tcatcatcta ccgctatgtg ctgtaccgtg ccgaccgtgc ggcgggggag
      301 atagagttcg ccgccgagcg gctcatggcg tggctcgacg aaagacagag gaacaccgat
      361 aatagagagg ggagtccagc atgaagacac gcgtatctct tttgatcgcg gcgcttgccg
      421 tcgcggtatg cgccgcctgt tcgtcggccc agccggccag cgagacgaag gccgatgagg
      481 gaacttccgc caccgccgag gctacggcgg ccaagccgaa cgcgggcgcg gccaagacgc
      541 aggatgagat cgacgaattc gtttccgcag cgcgcgaagg cgacctcaac gcggtcaaga
      601 ccttctacaa caccggcggc gacatcaaca aaccgaacgc ccgcggccag acggcgctca
      661 tgtgggccgc ccagcgcggc cacctcgagg tggtgaagtt cctcgttcag ttgggcgcca
      721 atgtgaacgc ggtcgatgcc gaccgtcagg aaccggtgtt gatgtgggcg accaagaagg
      781 ggcacaagga agtggtcgag ttcctcatcg cgagcggcgc cgatgtcaat cagggcgacc
      841 agttcaagga aacgcccctc atggacgcgg cacgctacgg ccacaaggac ctttgcgaac
      901 tgctcctcaa caaacgggcc cgcgtcaacg ataccgacgt caacggcgaa tcggcgctca
      961 tgtgggccgc cgccaacggc gtggccgagg tggtgaagtt cctcatcggc tacggcgccg
     1021 acatcaaccg catcaactac aagaccggcg aaacggcgct cggtaaggcg aagaacaaag
     1081 ggttcaacga tatcgtcgcg atgatgcaac ggcaggcgca ggtcgagggt aacctcatta
     1141 agcggccgcc gcgtcccgag gataaggact acgtcaacga gccgcccgac gagaaagaac
     1201 tggcgcgcta ctggggcggc cgcgaatcgg cgcttcccga gatgctcgac tcgaagatca
     1261 tcggcatcca catgaactcg ttcatcccga acatcaccca gtgctatcag gagcggatac
     1321 agcagggcga caagaacctc atggggcaga tgtggctcaa ggtccgcgtg gcggggtccg
     1381 gcaagatcat cgacattttc ttcgagaccg agaagtacca gtcgtcgctc ttcggcgact
     1441 gcatcctcga cgccatccgg aagaaggact tcccgatgtt ctatcagggg ctcatggagt
     1501 tcaagtacag ctacacgctg taaggtccgc tgttcccaat tccttatcag cgcgcaaaac
     1561 tgttgactcc ttatgccaat ttgctactat tgcggtgttt cagaaagcgg gggtgtccat
     1621 gaatcgtcta cttgtgtgtt atttctgcgg actgttcgtc cttctcggtt gcgggacggt
     1681 gcctactcaa aaaacagcgg aagacacaag tgcttcaaaa gaaactcggg ttcgacaggt
     1741 tgcagaacga ttcatgaaag ggaataggcc cacagatgat tggaaagatg atattgtgaa
     1801 gagttatctt gaaaagaata ctccagagac tgagtgccat atgatatgtg aaaaagatga
     1861 aaatgcctgc gcggaaaaga aggagggact taatcgacct cattgggtcg tgcgatatac
     1921 accggaatgc gttcgtgaca agaaatgcga aggaggcttt ttttcagtct atatagatga
     1981 cgagacagaa agggttatct gcgtttgggg agcaaagtga aatcccatct cggccccatc
     2041 ctcggcgcgg tcgccggaat aattgatgtg ataccgatgc tggcgcaagg gctgccgtgg
     2101 gaggccgacg cctccgcctt ctgcctgtgg gtgatcgccg gcttcttcat ccagcggacc
     2161 gacctgcaac tgcccgcccc gctcaaaggc atcgccatat catttcttct gctgatcccc
     2221 gccgccatcc tcatcgccgc caaggaacca ttttccctta tacctatatc tataatgacg
     2281 ctcctcctcg gcgccgccct cggctatgca attaaaaagg tgaccgcatg atgctcctcg
     2341 tcgtgaaata cgccgcgacg gcgctggtgg tggtcgtcgt ttcggaattc gccaaacgct
     2401 ccgataaact cggcgccctc atcgcgtcgc taccgctcgt caccatcatg gtcatgttct
     2461 ggctctacgc cgagaagcag gggaccgaga aagtggcgaa ccacgcctat tacaccttct
     2521 ggtatgtcat cccgaccctg ccgatgttcc tcctgatgcc gtggctcctg cggcgcggca
     2581 ccggcttctg ggcagccctc ggcctatcgg tactgctgac ggccgcctgt ttcgtcctga
     2641 cggcgttcat cgccaaacgg ttcggcgtcg aattgatacc gtgatcggtc ccgttcactt
     2701 cgcctgaaaa tagtcgttct gattggtcga gcagagcacc cctttccaga gcgcttcttc
     2761 ggggtatatc ttgcgccgtt cgatggtggc gagcgctatc ggcacatggg tgaagaagtt
     2821 gttccagtgt ccgatgaggc agttggtctt ccccgccatc gcggcgtgag ccgcatgctc
     2881 ggcgagatga tggcagaata tcgcgtcggt cccctgcgcc gcgacgctcc ggatgagata
     2941 ggagggatcg aagtacttga tggagaaatc gagtttccgc tccttgaagt accggttcat
     3001 ctcgtctttg aggaacaggc cgatatcctt gtgcaggaca ttgcccgatg cgtccttcgc
     3061 cttgtccccc gcgaagaact tctggcccgc accctcggcg acgaccatga ccgcgtggtg
     3121 attcttctcc agacagcggc cgatggcggc gagcag
//
LOCUS       DAOQUE010000345         3144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_35787_length_3144_cov_3.851732, whole genome shotgun sequence.
ACCESSION   DAOQUE010000345 DAOQUE010000000
VERSION     DAOQUE010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35787_length_3144_cov_3.851732"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKH10_10055"
     CDS             <1..352
                     /locus_tag="PKH10_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28506.1"
                     /translation="GASFQWLDAESAAGILRVQSLYLLDRAASAFIIGGWAEAGITAG
                     DDRSGFTGAVSGQFLWSTRKQMYATVNLRAGIYINPHEQARISLGPGAGRNFISNDTF
                     FFDIILALEVYLWN"
     gene            343..2679
                     /locus_tag="PKH10_10060"
     CDS             343..2679
                     /locus_tag="PKH10_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNT28507.1"
                     /translation="MELTTSLLRSVERPGRYFSPVIMTGALQPPPADAPKILVVFPDL
                     FEIARSHEGIKILRHLFRRYGAAVDYVFAFAADMEARLRREGRLISFLTGRDWRDFDL
                     IAVSFQFQLQFPTFLRMLDVAGIPVRADERGEKDPVVMAGGPVLCNPEPMRDFIDHAY
                     IGEVEPHAERIVDILRREKGRAGRIAAFLDELPPRRAIATDLNDLTLVPDEQPIFGLQ
                     TVHDRYTAEIQRGCTRGCRFCMAGVVYRPHRERHATTVLELLRRDVVAGGYREAGFLS
                     LSAGDHSRIEEILRSARETAERQFSVSLPSLRVETLTDRMVALAAAGRRSGFTLAPES
                     GSERLRRVVNKGNTTADLIASVQRIFDAGWCHLKLYFMLGLPGEEDVDIEETIALLRE
                     VCRIARTYGGRASVTASFSTFVPQPFTPFQWEGMASLDEIGRKQRMLIDALKKCRNLK
                     LSWHNRFQSRVEGMLTRADRRIGAAIAEVARRMTGLQSWDEGFDFALWSEAVAHAGLS
                     DEDLCGPRPIEEPLPYEYVDMGVTREFLLAEREKAFRGEQTPDCALGGVCVSCGVCDH
                     DRIAPRVEGEGAVEVIAGEPATPQEKEKFNRGNATPWIFSFEKKGRALSIGHLDLVEF
                     LLKGFTREKVAILYSEGFNPTPRFSLVDPLPVGVEAEEEYGVVWLTDAHDGADLTKRF
                     NALYEGTGIRFHEFRPVEREKIKETERAIREAPLRYACRFASPDEARRFLSLHTGLDA
                     GLDGATLLLRHPPEKGSVMKLFAGFDGEYHIVRERRTP"
     gene            2676..>3144
                     /locus_tag="PKH10_10065"
     CDS             2676..>3144
                     /locus_tag="PKH10_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNT28508.1"
                     /translation="MRSRLAPTPSGYLHIGNAFSFIVTWTLARRLGGTLLLRIDDLDT
                     DRKRPDFVADIFDNLDWLGLDRQEGPRDIAEFDAAWSQDRRMPLYRAAMERLKTIPGT
                     LFACECSRGQLAHSGATGRYPGACRDKGLSLDAPGTKLRFRAPDDATITVSGSA"
BASE COUNT          547 a         1023 c         1030 g          544 t
ORIGIN      
        1 cggcgcgtcg ttccagtggc tcgacgccga gagcgcggcg gggatactgc gcgtgcagtc
       61 gctctacctg ctcgatcgcg ccgcgtcggc attcatcatc ggcggatggg ccgaggcagg
      121 catcacggcg ggcgacgacc ggagcggctt caccggcgcg gtgagcggcc agttcctctg
      181 gtccacgcgg aagcagatgt acgccaccgt gaacctccgg gccggcatct acatcaaccc
      241 gcacgaacag gcccggatat cgctcggccc cggcgccgga cgcaacttca tcagcaacga
      301 caccttcttc ttcgacatca tcctcgcgct ggaagtctat ttatggaact gaccacctcc
      361 ctgcttcgtt cggtggaacg accgggccgc tatttctctc ccgtcatcat gaccggcgcg
      421 cttcagccgc cgccggcgga cgcgccgaag atactggtgg tattccccga cctgttcgag
      481 atagcgcgaa gccacgaagg catcaagata ctgcggcacc tgttccgccg gtatggcgcg
      541 gcggtcgatt acgtgttcgc cttcgccgcc gacatggagg cgcggctacg gcgcgaaggg
      601 cggctgataa gtttcctcac cggtcgcgac tggcgcgatt tcgatctcat cgccgtttcg
      661 ttccagttcc aacttcaatt cccgaccttc ctgcggatgc tcgatgtcgc cgggatcccg
      721 gtgcgggccg acgagcgtgg ggaaaaggac cccgtcgtca tggcgggcgg accggtcctg
      781 tgcaaccccg aaccgatgcg cgacttcatc gaccacgcct acatcggcga ggtcgaaccg
      841 cacgcggaac gcatcgtcga catcctgcgg cgcgaaaagg ggcgcgccgg gcgcatcgcc
      901 gcctttctcg acgagttgcc gccgcggcgg gccatcgcga ccgacctgaa cgaccttacg
      961 ctcgttcctg acgaacagcc gatattcggt ctgcagaccg tccacgatcg ctacaccgcc
     1021 gagatacagc gcggctgtac gcgcggatgc cggttctgca tggcgggcgt cgtgtaccgg
     1081 ccgcaccggg aacggcacgc cacgacggtg ctggaactcc tgcggcggga tgtcgttgcg
     1141 ggcggttacc gcgaggcggg gttcctctcg ctctcagcgg gcgatcacag ccgcatcgag
     1201 gagatactgc gatcggcgcg cgaaacggcg gagaggcagt tctcggtatc gctcccttcg
     1261 ctccgtgtcg agacgctgac cgatcggatg gtggcgctcg ccgccgccgg gcgtcggtcc
     1321 ggtttcacgc ttgcccccga atcgggaagc gaacggttgc ggcgcgtggt caacaagggt
     1381 aatacgaccg ccgaccttat cgcgtcggtg cagaggatat tcgacgccgg ctggtgtcac
     1441 ctcaaactct acttcatgct cggcctgccc ggcgaggagg atgtcgacat cgaggagacg
     1501 atagctcttc tgcgcgaggt ctgccgcatc gcgcggacct atggcggccg tgcctcggtg
     1561 accgcgagtt tctcgacctt cgtgccccag cccttcaccc cgttccagtg ggaagggatg
     1621 gcgtcgcttg acgagatagg gcgcaaacag cggatgctga tagacgcgct taaaaaatgc
     1681 cgcaacctca aactgtcgtg gcataaccgc ttccagagcc gcgtcgaggg gatgttgacg
     1741 cgggccgacc gccgcatcgg ggccgccatc gccgaggtgg cgcgccgcat gaccggcctg
     1801 cagagttggg acgaggggtt cgatttcgcg ctgtggagcg aagcggtggc gcatgccgga
     1861 ctttcggatg aggacctgtg cggtccgcgc ccgatcgaag agccgcttcc gtacgaatat
     1921 gtcgacatgg gggtgacgcg cgaattcctg ctggcggaac gcgaaaaagc gttccggggc
     1981 gaacagacgc ccgactgcgc cctcggcggc gtctgcgtct cgtgcggggt ctgcgaccac
     2041 gaccgcatcg cgccccgcgt cgagggagag ggggccgtcg aggtcatcgc cggtgagccg
     2101 gcgacgccac aggaaaaaga gaaattcaac cggggtaacg cgacaccgtg gatattctcc
     2161 tttgaaaaga aaggccgcgc gctctccatc ggtcatctcg atctcgtcga gttcctgctc
     2221 aagggcttca cccgcgaaaa ggtggcgata ctctattcgg agggtttcaa tccgacgccg
     2281 cgcttcagtc tcgtcgatcc tctgccggtc ggggtcgagg cggaggagga atacggcgtg
     2341 gtctggctga ccgatgcgca cgacggagcg gacctgacga agcggttcaa cgcgctgtac
     2401 gaggggacgg ggatacggtt ccatgaattc cgtccggtgg agcgggaaaa gatcaaggag
     2461 accgaacggg ccatccgcga ggcgccgtta cggtacgcgt gccggttcgc ttcgcctgac
     2521 gaggcgcgcc ggttcctctc ccttcatacc ggtcttgacg ccggtctcga cggagcgacc
     2581 ctgcttctgc gtcacccgcc ggaaaaggga tcggtgatga aactgttcgc cgggttcgac
     2641 ggcgaatacc acatcgtcag ggaacgccgc acgccatgag atcgcgtctt gcgccgacgc
     2701 cgtccggcta cctgcacatc ggcaacgcct tttccttcat cgtcacctgg acgctcgccc
     2761 ggcgactcgg cgggacgctc ctcctgcgga tagacgatct cgataccgac cggaaacggc
     2821 ccgatttcgt cgccgacatc ttcgataacc tcgactggct cggactcgac cggcaggagg
     2881 ggccacgcga catcgccgaa ttcgacgcgg catggtcgca ggaccgtcgg atgccgctct
     2941 accgcgcggc gatggagcga ctcaagacca taccggggac cctgttcgcc tgcgagtgca
     3001 gccggggaca actggcccac agcggcgcga cgggacgcta tcccggtgcc tgccgtgaca
     3061 aggggttatc gcttgatgcg cccggcacga aactgaggtt ccgcgcgccg gacgatgcga
     3121 ccatcaccgt ctcaggctcc gccg
//
LOCUS       DAOQUE010000346         3132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_35984_length_3132_cov_4.485863, whole genome shotgun sequence.
ACCESSION   DAOQUE010000346 DAOQUE010000000
VERSION     DAOQUE010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35984_length_3132_cov_4.485863"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PKH10_10070"
     CDS             <1..876
                     /locus_tag="PKH10_10070"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNT28509.1"
                     /translation="LGRPHATVVFDTDLSRVRRELDALRPEAVFNLVESFDGKGALQY
                     IAPAMLEQWGFRRTGGTAESLFLTTDKLLTKELLALKGIPTPGHFAHGRHERVEPGTR
                     YIVKPIDEDASLGIGPDSIVTPANATELQSLIDRKEREYGVRHFAEQFIDGREFNLTV
                     LGLNGAPQVLSPAEIRFIDFGETRPKIVDYRAKWDEGSFECKNTRRSFDLPSEDAPLL
                     ARLADIAVLCWRDLRLSGYARVDLRVDGAGMPWVLEVNVNPCIAPDGGVAAACSHDGI
                     GYASFIERVLAEALR"
     gene            940..1584
                     /locus_tag="PKH10_10075"
     CDS             940..1584
                     /locus_tag="PKH10_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HNT28510.1"
                     /translation="MLSVHSIFSSIQGEGRFAGYPTAFLRLAGCNLSCAWCDAHEAAR
                     SVGAFPLSVAEAFARLTASGLRDTCITGGEPLLQGTAVAELLDRLLPLRRVVIETNGS
                     LPIVSLKRRFPSVSFSVDWKMPSAGEPSFQEEILADLGPEDWIKFVVADRRDLDHVAT
                     ALPAVASKGIESFVSPVFERGSAWFAEVGAFVTTLPARHPVRFQLQLHKMLGIP"
     gene            1660..2853
                     /locus_tag="PKH10_10080"
     CDS             1660..2853
                     /locus_tag="PKH10_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNT28511.1"
                     /translation="MRRSSTVFLLVAIVSAVACEKFMENDVNVAPRDVTEVSFLTLNT
                     GLDPQGVLNYADRKKALPQALYDTGADILCLQEVWKEADQKEIIANLKEVFPYRFYKT
                     TKQEALDPIPAACTEEDLTPMAMCYFSNCSETDPSDVMAIGLCLLTNCVEEITGIPAE
                     CRDCLIGAISSGSADLTGIMAECTGEQEAVEMAQDGNNGLLLLSNLPLKGAKLEKFDS
                     FFYQRAYIYAIATHPLFGDIEVVCTQFTDAVPDMAYEGTVGSWQGETAAQAQAIIDLA
                     PGKGTVLRALMGDLAAGPAIGRDIKAKNPSIYEMLYYAYYYNPFIEPDEAAPACTLCA
                     DNVLSAAGTQDTITDHVLFTPFDGLNLSAERVLDAGFVLTDKSGKEKTVQYSDHYGIK
                     ATLSR"
     gene            2863..>3132
                     /locus_tag="PKH10_10085"
     CDS             2863..>3132
                     /locus_tag="PKH10_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HNT28512.1"
                     /translation="MDIRKFTGKLDGIDFLDLLMLDIYGSIRHVTIPKGYITKKVLNE
                     GIGFDASNFGFAKVHDSDMVAVPDLATGFVGMVQDKKVLRCLCDVV"
BASE COUNT          584 a          967 c          954 g          627 t
ORIGIN      
        1 ctcggccgac cgcatgcgac ggtcgtgttc gacaccgacc tttcccgcgt gaggcgggaa
       61 ctcgacgcgt tgcggcccga agcggtgttc aaccttgtgg agtcgttcga cgggaaggga
      121 gcgctccagt atatcgcgcc ggcgatgctc gagcagtggg ggttccgccg taccgggggg
      181 accgccgagt ccctgttcct caccaccgat aaattgctca cgaaggaatt gctcgcgctc
      241 aaaggcatac cgacgccggg tcatttcgct cacggccgcc acgagcgggt agagcccggt
      301 acgcgctaca tagtcaagcc gatagacgag gatgcgtcgc tcggcatcgg tcccgacagc
      361 atcgtcacac ctgcgaatgc cacggaacta cagtcgctca tcgatcgcaa ggagcgcgaa
      421 tacggtgtcc gtcacttcgc cgaacagttc atcgacggtc gcgagttcaa cctcacggtg
      481 cttggcctga acggcgcgcc gcaggtgctc tctcccgccg agatacgctt catcgacttc
      541 ggcgagacgc gccccaagat cgtggattat cgcgccaaat gggacgaggg ttcgttcgaa
      601 tgcaagaaca cgcggcgctc attcgatctc ccgtctgagg atgcgccgct ccttgctcgg
      661 cttgccgata tcgcggtgct atgctggcgc gacctgcggc tttcgggtta cgcgcgggtc
      721 gatctgcgtg ttgacggcgc ggggatgccg tgggtgctcg aggtcaatgt caatccctgc
      781 atcgcgcccg acggcggggt cgccgccgcc tgctcccatg acggcatcgg gtacgcctcg
      841 ttcatcgagc gggtgctcgc cgaggcgctc cggtagttct ctttactttt ccgggcccgt
      901 cgcgtataac gatgacctca cggtcatcgg gcggtcgtca tgctatcggt ccattcgata
      961 ttctcctcga tacagggcga aggtcgcttc gccggctacc cgaccgcgtt cctgcgcctt
     1021 gcgggatgca acctttcctg cgcgtggtgc gacgctcacg aggcggcgcg aagcgtcggg
     1081 gcgttccctc tttctgtcgc cgaggcgttc gcccgactga ccgcgtcggg gctccgcgat
     1141 acctgcatca ccggcggtga accgcttctt cagggaacgg ccgtggccga gttgttggac
     1201 cgcctccttc cccttcgccg ggtggtcatc gagacgaacg gttcgctccc gatcgtatcg
     1261 ctcaagcgcc ggttcccgtc ggtatctttt tcggtcgact ggaagatgcc ttccgccggg
     1321 gagccctctt tccaagaaga gatactggcg gacctcggcc ccgaggactg gataaagttc
     1381 gtcgtcgccg accggcgcga cctcgaccat gtggccaccg cgttgcccgc cgtcgcgtcg
     1441 aaaggcatcg agtcctttgt gagtcctgtg ttcgagcggg gttcggcatg gttcgccgag
     1501 gtcggcgcat tcgttaccac tctcccggcg cgccatccgg tccggttcca actgcaactg
     1561 cacaagatgc tagggatccc gtgagttcac aaaaatactt tcttttttct tttttcggat
     1621 actatgagga taaggaagaa cgctagagag gaggaagaca tgcgcagatc atcgacggtg
     1681 ttcctgttgg tcgcgatcgt ttcggcggtc gcctgcgaaa agttcatgga gaacgatgtc
     1741 aatgtcgcgc cgcgcgacgt gaccgaggtc tcattcctta ccctcaacac cgggctcgac
     1801 ccgcaggggg tgctcaacta tgccgatcgc aagaaggcgt tgccgcaggc gctgtacgac
     1861 accggtgccg acatcctgtg cctgcaggaa gtgtggaaag aggccgacca gaaggagatc
     1921 atcgcgaatc tgaaggaagt gttcccgtac cgcttctaca agacgaccaa gcaggaggcg
     1981 cttgatccga taccggcagc ctgtaccgaa gaggacctca cgccgatggc gatgtgctat
     2041 ttcagcaact gtagcgagac cgaccccagc gatgtcatgg ctataggcct ctgtctcctc
     2101 accaactgtg tggaggagat caccggcatc ccagccgaat gtcgcgactg cctcatcggc
     2161 gccatttcca gcgggagcgc cgatctgaca ggcatcatgg cggaatgtac gggcgagcag
     2221 gaagcggtcg agatggcgca ggacgggaac aacgggctcc tgcttctgtc caacctgccg
     2281 ctcaaagggg cgaaactcga gaagttcgat tctttctttt atcagcgtgc ctacatctat
     2341 gcgatagcga cccatccgct gttcggcgat atcgaggtcg tctgtaccca gttcaccgat
     2401 gccgtgccgg acatggccta cgaaggaact gtcggttcgt ggcagggcga gacggcggcg
     2461 caggcgcaag cgatcatcga cctcgcgccg ggcaagggca cggtcctgcg cgctctcatg
     2521 ggcgatctgg ccgccggccc ggcgatcggt cgcgacatca aggcgaagaa cccctcgatc
     2581 tacgagatgc tttactacgc ctactactac aatcccttca tcgagccgga tgaggccgcc
     2641 cccgcctgca ccctgtgtgc cgacaatgtc ctttccgcgg cggggacgca ggacacgata
     2701 acggaccatg tgctcttcac gccgttcgac ggtctgaacc tctcggccga acgggtgctc
     2761 gacgcgggat tcgtgctgac cgacaagagc ggcaaggaga agaccgttca gtattcggac
     2821 cactacggca tcaaggcgac cctttcacgg tgaggtgaaa cgatggacat cagaaaattc
     2881 accggcaaac tcgacggcat cgacttcctc gacctgctga tgctcgacat ctacggcagt
     2941 atccgccatg tcacgatacc gaaaggatac atcaccaaga aggtcctcaa cgaggggatc
     3001 ggtttcgatg cctcgaactt cggcttcgcg aaggtccacg attccgacat ggtggcggtc
     3061 cccgaccttg cgaccggctt tgtcggcatg gtgcaggaca aaaaggtgct tcgctgcttg
     3121 tgtgatgtgg tc
//
LOCUS       DAOQUE010000347         3131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_36002_length_3131_cov_4.322172, whole genome shotgun sequence.
ACCESSION   DAOQUE010000347 DAOQUE010000000
VERSION     DAOQUE010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36002_length_3131_cov_4.322172"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PKH10_10090"
     CDS             <1..631
                     /locus_tag="PKH10_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28513.1"
                     /translation="IPADADAPLTDDEQPDTDTGDCNTVNFGKNCKTDTECGTCLICV
                     NAKCAQGCLSDADCTAYAETTCNRKLSRCLNTTAIFGTGICNETNCPSGCCYATKGFQ
                     SLKCLGTAQLQTCGICKQGEIYMDGKQCVPAACKVGETKCQTYNATEPRAECFECKTG
                     DLVCYDNPSCQTGGLILPLEHRTCIPAGEQCSEGTTCCSGTPCIRGYCY"
     gene            633..1280
                     /locus_tag="PKH10_10095"
     CDS             633..1280
                     /locus_tag="PKH10_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28514.1"
                     /translation="MKNILLLPIIMFAVALLLFACENETHLRLVPDQGGVTDAEQETA
                     DDAAVTTPDNDTAAPADEDVIIPPDEDTAGASCELDKECADGGICVSGSCTAGCAEDG
                     DCAAYPGTSCNTETHRCINETANDGACGDTRCPTGCCYAVRGFRELKCIIPPSAEYCG
                     ACPQGQVFMGRAECVPSACSTGDTLCQTYNAYDPRSACFACKTGDLICYDDPACN"
     gene            1280..1990
                     /locus_tag="PKH10_10100"
     CDS             1280..1990
                     /locus_tag="PKH10_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28515.1"
                     /translation="MELIDTGKCIVCGAFFLTISFLFLSSCESNTHQTAVDAHADSDG
                     IADQTENDPAIMPDDDSVADIDVADDDTLIIDNSAPDADLPGDCDTTNLGKNCKTDIG
                     CGACLICANARCSEGCTNDGDCDAYPGTICNKKLARCLNIAASDGACNETNCTDGCCY
                     AAKGFRSLECASTATLATCGVCKQGEIYMDGKQCVPAACSTGDTLCQTYNAYDLRPTC
                     FTCKTGDLICYGDPACLH"
     gene            1990..2388
                     /locus_tag="PKH10_10105"
     CDS             1990..2388
                     /locus_tag="PKH10_10105"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNT28516.1"
                     /translation="MKLIDTGKCIVCGKDNPHGFQLKISTDREKGRAWCTVTIPEKYI
                     GWERFIHGGITSMLLDEMMAHAAIALGRFCVTAEMKVRFKRPVLPDVPLSVEGEVISR
                     NDRIITTKAVMKDGDTVLAEAEGRMVIPRS"
     gene            complement(2443..>3131)
                     /gene="tpiA"
                     /locus_tag="PKH10_10110"
     CDS             complement(2443..>3131)
                     /gene="tpiA"
                     /locus_tag="PKH10_10110"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNT28517.1"
                     /translation="SFFGAIGDLCRGLNDREVVVTPSFPYIAPAAALAKGRGITIAAQ
                     DFHPEENGAFTGEVSLAMLADLGVRTVLIGHSERRHVMGETHEHIRKKVAFAQKKGFR
                     IIFCIGETLAQRQKGELFAVIDHQLSTAFDDNLTCLPGDMVIAYEPVWAIGTGVNATG
                     AQAQEMHDYIRHFMDKHYGRKEISILYGGSVKPDNVKELMMMPDIDGALVGGASLKAE
                     SFSKLIFWDK"
BASE COUNT          662 a         1004 c          902 g          563 t
ORIGIN      
        1 cattcccgcc gatgccgatg cccccctcac tgacgatgaa caaccggata ccgacaccgg
       61 cgactgcaac accgtcaatt tcggcaagaa ttgcaagacc gacaccgaat gcggcacctg
      121 cctcatctgc gtcaacgcaa aatgcgcaca gggatgcttg agcgacgccg actgcaccgc
      181 ctatgccgag accacctgca acagaaaact ttcccggtgt ctgaacacca cggccatttt
      241 cggcacgggt atctgcaacg agacgaactg cccgtcaggc tgctgttacg cgacgaaggg
      301 cttccagtcg ctcaaatgcc ttggaacggc gcaacttcag acctgcggca tctgtaaaca
      361 gggggagata tacatggatg gcaaacagtg cgtgccggcc gcctgcaagg tcggcgagac
      421 caaatgtcag acctacaatg cgaccgagcc acgcgccgag tgtttcgaat gcaagaccgg
      481 cgatctggtg tgctatgaca acccgtcctg ccagaccggc ggactcatcc ttcctctcga
      541 acaccgcacc tgcataccgg cgggcgagca atgttccgag ggtacgacct gctgcagtgg
      601 tacgccctgc atcagggggt attgttactg acatgaaaaa catactcttg ctccccatca
      661 tcatgttcgc cgtggcgctc ctgctgttcg cctgtgagaa cgagacccat ctgcgcctag
      721 tccccgatca gggcggcgtg accgacgccg aacaggagac cgccgacgat gcggccgtca
      781 ccacgccgga taacgacacc gccgccccgg ccgacgagga tgtcatcatc ccgcccgacg
      841 aggatacggc cggcgcctcg tgcgaactag ataaggaatg cgcggatggc ggcatctgcg
      901 tatcgggctc ctgcacggct ggctgcgccg aggacggcga ctgcgcggca tatccgggga
      961 cctcctgcaa caccgaaacg caccgttgta tcaacgagac ggcgaatgac ggtgcctgcg
     1021 gcgacacccg gtgcccgacc ggttgctgct acgccgtgcg cggcttccgc gaactcaagt
     1081 gcatcatccc gccttccgcc gagtactgcg gcgcctgccc gcaaggtcag gtgttcatgg
     1141 gccgcgccga atgcgtcccc tcggcctgtt caaccggcga caccctctgt cagacctaca
     1201 acgcctacga cccgcgctcg gcctgtttcg cgtgcaaaac gggcgatctt atctgttacg
     1261 acgaccccgc ctgcaactga tggaactcat cgacaccggg aaatgcatcg tctgcggggc
     1321 gttcttcctg acgatctcgt tcctttttct ctcatcctgc gaatcgaaca ctcaccaaac
     1381 agcagtagat gcccacgccg atagcgacgg catcgctgac cagacggaga acgatcccgc
     1441 gatcatgcca gatgacgatt ccgttgccga tatcgatgtt gccgacgacg acacacttat
     1501 tatcgacaac agcgcacccg atgccgatct gcccggagat tgcgatacta cgaatctcgg
     1561 caagaactgc aagaccgaca tcggatgcgg tgcttgcctc atctgcgcga atgcaagatg
     1621 ctctgaagga tgcaccaacg acggtgactg cgacgcctat cccgggacca tttgtaacaa
     1681 aaagttggcg cgctgcttga atatcgccgc ctcggacggc gcatgcaacg aaacgaactg
     1741 cacggacggc tgttgctacg ccgccaaggg gttccggtcg ctcgaatgcg catcgaccgc
     1801 caccctcgcc acctgcggtg tctgcaaaca gggcgagatc tacatggacg gcaaacaatg
     1861 cgtgcccgcc gcctgttcga ccggcgacac gctgtgccag acctacaacg cctacgacct
     1921 gcgtccgacc tgtttcacct gcaaaacggg cgaccttatc tgttacggcg accccgcctg
     1981 cctgcactga tgaaactcat cgacaccggg aaatgcatcg tctgcggcaa ggataacccg
     2041 cacggctttc aactgaagat atccaccgac cgagagaagg gccgggcgtg gtgcactgtc
     2101 acgataccgg agaagtacat cggctgggaa aggttcatcc acggcgggat cacctcgatg
     2161 ctccttgacg agatgatggc ccacgccgcc atagcgctcg ggcggttctg cgtcaccgcc
     2221 gagatgaagg tccgcttcaa acggccggtc ctgcccgatg tccctctctc cgtcgaaggc
     2281 gaggtaatat cgcgcaacga ccgcatcatc acgaccaagg cggtcatgaa ggatggcgac
     2341 acggtactgg cggaagccga agggcggatg gtgataccga gatcgtgagt cgtgagaagt
     2401 gagcggtgaa cggaggcggg gacgcgatcc atcgcgcccg atctatttgt cccagaatat
     2461 cagtttcgag aacgactcgg ccttgagcga cgcaccgccg acgagcgccc cgtcgatgtc
     2521 gggcatcatc atcaactcct tgacattgtc tggcttgacg ctaccgccgt agaggatgga
     2581 gatctccttg cgaccgtagt gtttatccat gaagtgccgg atgtaatcgt gcatctcctg
     2641 cgcctgcgcc ccggtcgcat tgacgccggt gccgatggcc cagaccggct cgtaggcgat
     2701 gaccatgtcg cccggcaggc aggtcaggtt gtcatcgaag gcggtggaaa gttgatggtc
     2761 tatcacggcg aacagttccc ccttctgccg ctgggcgagc gtttcgccaa tacagaagat
     2821 gatacggaac cccttctttt gcgcgaaggc gaccttttta cggatatgct cgtgggtctc
     2881 ccccatcaca tgacgccgtt cagaatgacc gatgaggacg gtgcggacgc cgagatcggc
     2941 gagcatcgca agactcactt cgccggtgaa ggcgccgttc tcctcggggt ggaaatcctg
     3001 cgccgcgatc gtgatgcctc gccctttcgc gagcgccgcg gccggcgcga tgtaggggaa
     3061 cgacggcgtc acgacgacct cgcggtcgtt cagccccctg cacaagtcgc cgatcgcacc
     3121 gaagaaggag g
//
LOCUS       DAOQUE010000348         3108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_36375_length_3108_cov_3.597773, whole genome shotgun sequence.
ACCESSION   DAOQUE010000348 DAOQUE010000000
VERSION     DAOQUE010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36375_length_3108_cov_3.597773"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..694
                     /locus_tag="PKH10_10115"
     CDS             38..694
                     /locus_tag="PKH10_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002182672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNT28518.1"
                     /translation="MICRYGQTDAPRIIVGAHYDTVRTTPGADDNASAVAGLLEIARL
                     LNQLKPRVKYPLELVAYDLEEPPYYNTKHMGSYRHAEKLSSDEEKVHFMISLEMIGYF
                     SDESGSQKYPSPFLKLMYPSKGNFILVTGTDGEVLDLFTEAFPRASKLPLETFEYRID
                     LSDHMNYRDLGFRAVMVTDTAFYRNPNYHEPTDTPDTLDYARMAEVVKGVFLTVASAE
                     "
     gene            770..1570
                     /locus_tag="PKH10_10120"
     CDS             770..1570
                     /locus_tag="PKH10_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28519.1"
                     /translation="MIRYFFIFAALLAVVVGCKDDTTKEDSCPNGTIYNDCGLSACGD
                     PTTASMCQGDILLYCGVPTDPCWSGSQKFEKDCAASGLRCFSADENGCGGMCLPVTDS
                     EPDEQPDDDESELDVDEYHDVDEYPDADCLMVACYDFTIKVSGAVANEGGTPIPGIAV
                     ALYDDGVISSPQFMSNEDGVWLIEYAYGECGGTDEGVVDAKVIATDIDGSTNGVYVET
                     VIDFSPWNVKNDEWVPPCINSDIYLEELGVQIVMLEQPDDDTLLTDAN"
     gene            1583..>3108
                     /locus_tag="PKH10_10125"
     CDS             1583..>3108
                     /locus_tag="PKH10_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28520.1"
                     /translation="MTRHLLFIMVVALLVVACNNARPRESIPDGNDALTDAEAAALAD
                     EDALLTDPDEETTDDDALLTADSDTAFDDGGDDLLVSDDDTGPVAFHWTLVHPHTDDY
                     GDTVFRGIDFPTVVVFKEALWVTGGENRTDVWRSSDGAAWEKRGSLPASASFPFPPRR
                     PAAAALGDAIFYVNESGVWRSEDGVDWTNIQPDPSFGVRRGMALVAHQGELWLIGGNG
                     GGVFFGDAWRSNDGIQWIQTGMWQTGAHSGNCTPVEGVAGPAAVSMGDRLLAVGGHYS
                     DSCSYTSGYPEIDYSYSVIHYSAQYGLYREEYGWTYPSVPESDDAPFGSHSLLVPFNG
                     EYWSFGGQQWGSDMVRYDRGPGGSGTIIYPGEIVPNHDILTTVEGFDWQLSSYTMPFY
                     EMAGYGAASFKEAIWLIGGNGSNAVWKGSFDPDDPVTDECVKGKYYTGDILIYADAAA
                     ENYRGYTAAGRVVIAGSVTDIEPLHCLREVETIEIGYADCDTCGVTELVSFTGLRNLE
                     T"
BASE COUNT          663 a          826 c          940 g          679 t
ORIGIN      
        1 gcaacgttat acgattgacg ccggtgaatt ccgaaacatc atctgccggt atggacagac
       61 cgacgcaccg cgcatcattg tgggagcaca ttacgacacg gtccggacca cgccgggagc
      121 cgacgacaac gcgagcgccg tcgcgggact tttggaaata gcgcggttgc tgaatcagtt
      181 gaaaccgagg gtcaagtatc cgctggagtt ggttgcctac gaccttgagg agcctccgta
      241 ttacaacacg aagcacatgg gcagttatcg ccacgccgag aaactgagca gcgacgagga
      301 aaaggtccat ttcatgatat cgctggagat gatcggctat ttctccgatg agtccggttc
      361 gcagaagtat ccgtcgccat ttctaaaact gatgtatccc agcaagggga acttcatcct
      421 tgtgaccggc accgacgggg aggtgcttga cctttttacg gaagcgttcc cgcgcgcttc
      481 aaagttgccg cttgagacct tcgagtatcg catagacctt tcggaccaca tgaactaccg
      541 cgacctcgga tttcgcgcgg tgatggtgac cgatactgcc ttctatcgga atccgaacta
      601 tcacgagccg accgataccc ccgatacgct cgactatgca cggatggccg aagtggtgaa
      661 aggggttttt ttgaccgtgg cgagtgcgga atgagccgga acggaaccat tcactgtaat
      721 aaaaggtcta tgtttctgtt accatagagg ggaatccgtg gagatgccta tgatcagata
      781 ctttttcatc tttgccgccc tgctggcggt ggtcgtggga tgcaaagatg atacaacaaa
      841 ggaagattct tgtcctaacg ggaccatata taacgattgt ggtcttagcg catgcggtga
      901 cccgacaact gcaagcatgt gtcaaggaga tattcttctt tactgtggcg tcccgaccga
      961 tccctgctgg agcgggtctc aaaaattcga aaaagattgc gccgcctcgg gtcttcgttg
     1021 tttcagcgcc gatgaaaacg ggtgcggggg gatgtgcctt cccgttactg actcagagcc
     1081 ggacgaacag cccgacgacg acgaaagtga acttgatgtc gatgagtatc acgatgtcga
     1141 tgaatatccc gatgccgatt gtcttatggt cgcctgctat gatttcacta taaaggtgtc
     1201 cggtgcggtg gcaaatgaag ggggaacgcc aataccgggt attgcggtgg cactgtacga
     1261 tgatggggtg atcagttcgc cgcaattcat gtctaacgag gatggtgtct ggcttataga
     1321 atacgcttat ggcgagtgcg gtgggactga tgagggggtt gtggatgcta aagtcattgc
     1381 caccgatatc gacggttcga cgaatggggt atatgtcgaa acggtgatcg atttctctcc
     1441 gtggaatgtc aaaaacgatg aatgggtgcc accgtgtatc aattccgata tttatctcga
     1501 agaattaggg gttcagatcg ttatgctcga acagcccgac gacgacacct tgctgaccga
     1561 tgcgaattag gggtaggcga ccatgaccag acatcttctt ttcatcatgg tcgtggcact
     1621 gttggtcgtt gcctgcaata atgcaagacc gcgggagagc attcccgacg ggaacgatgc
     1681 cttgaccgac gccgaggcgg ccgcgcttgc cgacgaagat gccctgctga ccgatcccga
     1741 cgaagaaaca actgacgacg atgctttgct gaccgccgat agcgacaccg ccttcgacga
     1801 tgggggagac gatcttttgg tatcggatga cgatacgggg ccggtcgcct tccattggac
     1861 gctcgtccat ccccataccg atgattacgg cgatacggtc tttcgcggga tagatttccc
     1921 gacggtcgtc gtgttcaaag aggcgctctg ggtgaccggc ggcgagaacc gcaccgatgt
     1981 ctggcgctca tccgacggcg cggcgtggga aaagagaggt tcgttgcccg cctccgcctc
     2041 tttcccgttc ccgccccgtc gaccggcggc ggcggcgctc ggcgacgcga tcttctatgt
     2101 caacgagtcg ggggtatggc gttcggagga cggtgtcgac tggacgaata tccagcccga
     2161 tccgtcgttc ggcgttcgtc gcggcatggc gctcgtcgcg catcagggtg agttgtggct
     2221 catcggtggc aacggcggtg gagtgttttt cggcgacgca tggcgttcca atgacggcat
     2281 acaatggata cagacgggga tgtggcagac cggcgcgcac tccgggaact gcacaccggt
     2341 cgaaggggtg gcgggcccgg ccgccgtttc aatgggtgac cggctcctcg cggtcggggg
     2401 tcattacagc gattcctgtt cctatacgag cggctatccg gagatcgact actcctactc
     2461 ggtcatccac tactcggctc agtacggact gtaccgcgaa gaatacggct ggacctaccc
     2521 gtcggtgccc gagtccgacg atgctccctt cggcagtcac tcactgcttg tgccgttcaa
     2581 cggcgaatac tggagtttcg gcggccagca gtgggggagc gatatggtac gctacgaccg
     2641 cggtccgggc ggttccggca cgatcatcta tccgggcgag atagtgccca accacgatat
     2701 acttaccacc gttgaggggt tcgattggca actttcctcc tatacgatgc cattctacga
     2761 gatggccggg tacggcgccg catctttcaa ggaagccatc tggctcatcg gcggcaacgg
     2821 ctcaaacgcg gtgtggaaag ggtcgttcga tcccgatgac ccggtcaccg atgaatgcgt
     2881 gaaggggaaa tactacacgg gcgacatcct gatatatgcc gatgccgccg ccgagaatta
     2941 tcgcggctat acggcggcgg ggcgcgtcgt tatcgccgga tcggtgaccg acatcgagcc
     3001 tctgcactgt ctgcgcgagg tcgaaacgat cgagatcggc tatgccgact gcgatacctg
     3061 cggcgtgacg gaacttgttt cgttcacggg actacgcaac ctcgaaac
//
LOCUS       DAOQUE010000349         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_36465_length_3103_cov_4.750656, whole genome shotgun sequence.
ACCESSION   DAOQUE010000349 DAOQUE010000000
VERSION     DAOQUE010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36465_length_3103_cov_4.750656"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKH10_10130"
     CDS             complement(<1..199)
                     /locus_tag="PKH10_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28521.1"
                     /translation="MRLKRYLTSDPAWVFTTNFCEGFPYTVIRVISNFYLATTQVAVE
                     FIGLTALFGIPWTFYNLLFEIL"
     gene            complement(258..1460)
                     /locus_tag="PKH10_10135"
     CDS             complement(258..1460)
                     /locus_tag="PKH10_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT28522.1"
                     /translation="MKLYRVGGSIRDEILGLPKGDDDLVVVGATEQEFLSRFPGAERV
                     GKAFPVYLVDGAEYAFARRERKSGPGYRGFDTDADPSVTLVEDLKRRDLTINAIAQDL
                     ETRELIDPWGGRKDLERKVLRHISDAFAEDPLRLYRVARFAALLPDFTLEAATLELMK
                     RLVAELDTLSPERVWLECERALAARAPWRFFTILRDAGALPVHFPELHALVGLTPGPD
                     RYHEGETDTFDHALRLLHRLPTADPHLRFAALCHDLGKGLTDPAILPHHYGHDEAGVE
                     LVTALADRLKIPNSFRLAGELAARHHMAAAKLPVMRAGKAVTLLRKLRRFPGGGIAGY
                     LTLLHADSAGKERDLTPLAPEFERILDIPLPEKYHDLGPKSGEILLNLQAEAFNELRE
                     KVLSEPNK"
     gene            complement(1470..>3103)
                     /locus_tag="PKH10_10140"
     CDS             complement(1470..>3103)
                     /locus_tag="PKH10_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28523.1"
                     /translation="RVQMGPEKRSRVVTDEDTVVELCADPNGDEDADGIPNGVDGSED
                     RDLDGAPNCLDPDSDGDGILDGTECPEQPCANSDADDTPDFLDKDSDNDGLSDKEEVQ
                     EGTSPTKKDSDGDGSDDLAEIVYGSDPLDDEDTIPDGLFFVVLPYNAPEEVTRTLTFS
                     TLIEAIDVQIIIDISGSMNDEIAQVQNGIKDNIIDPVKAEFPQEGFASFGVSRISWNG
                     TTKFLRQKQTFSVDAVKSAVDKVNEDAFNTSVGANELHSEVLYQLAAGEEFHGTAKFC
                     LPPAMGGCNQNMIPSAVVDIAPANCTGEIGTVGHACFRKKSMPIYIEITDEEREDCDP
                     NGVQVSAECYWVAGAGFMNGHTFEEALAVMGGIGAKFIGINTWHEKTADGEANAGIDP
                     QPDMEMLAQYTGSVDKDGKPFIYHTATPTGEGMPDQIAQAIKDLITFIDMNVTTGKMS
                     DDECDGISAAEFVKSSTTVAADPEDGISGKDGTTFFSVKQGTTVTFDVRFKNDFCQNT
                     TTQPVVYDALVTVLGNGSYLSNRLVKVIVPGIDAQ"
BASE COUNT          473 a          975 c         1028 g          627 t
ORIGIN      
        1 tgaggatctc gaacagcagg ttgtagaagg tccacgggat accgaacagc gccgtgagcc
       61 cgatgaattc gaccgcgacc tgcgtcgtgg cgagatagaa gttcgatatg acgcggatga
      121 ccgtgtaggg gaacccctcg cagaaattgg tcgtgaagac ccatgccggg tcgctcgtca
      181 gatagcgttt cagtctcatg ggcgacttat gacagaaaaa gggaggcgta gcaagaaaac
      241 ctttttacgt gttcaggtca cttgttcggt tcgctgagca ccttttcccg cagttcgttg
      301 aacgcttcgg cctgcaggtt gaggaggatc tctcccgact tgggaccgag gtcgtggtat
      361 ttttcgggga gcgggatatc gaggatgcgc tcgaattcgg gggcgagcgg cgtgaggtcg
      421 cgttccttcc ccgccgagtc ggcgtgcagg agcgtgaggt agccggcgat gccgccgccc
      481 ggaaagcggc gcagtttcct gaggagcgtc accgccttcc ccgcccgcat caccggcagt
      541 ttcgccgccg ccatgtggtg ccgcgccgcc agttcccccg ccagacggaa actgttcggt
      601 atcttcagac ggtcggcaag cgccgtgacg agttccaccc ccgcctcgtc gtggccgtag
      661 tggtgcggca ggatggccgg atcggtcagc cctttgccga gatcgtggca gagcgccgcg
      721 aaccgcaggt gcgggtcggc cgtcggcagg cggtggagca gtcgcagcgc gtggtcgaag
      781 gtatcggtct ccccctcgtg gtagcggtcg ggccccggcg tgaggccgac aagcgcgtgc
      841 agttcgggga aatggaccgg cagcgcgccg gcgtcacgga gtatcgtgaa gaatcgccac
      901 ggggcgcggg cggcgagcgc ccgttcgcat tcgagccaga cccgttcggg cgagagggtg
      961 tcgagttccg cgacgagtct cttcatgagt tcgagcgtcg cagcctccag cgtgaagtcg
     1021 ggcaggagcg ccgcgaaccg cgcgacacgg tagagccgta gcgggtcctc ggcaaaggcg
     1081 tcgctgatgt ggcgaagcac cttgcgttca aggtccttcc ggccgcccca cgggtcgatg
     1141 agttcacgcg tctcaaggtc ctgcgcgatg gcgttgatgg tgaggtcgcg ccgtttcagg
     1201 tcctcgacga gcgtcaccga cgggtcggcg tcggtgtcga acccgcggta gccggggccg
     1261 ctcttccgct cccgccgcgc aaaggcgtat tccgcgccgt caacgagata gaccgggaac
     1321 gccttgccca cgcgctccgc gccggggaac cggctcagga attcctgttc ggtcgcgccg
     1381 accacgacca gatcgtcgtc gcccttcggc agaccgagta tctcgtcgcg gatcgagccg
     1441 ccgacacggt agagtttcat ggacggtcgt tactgcgcgt cgatgcccgg gacgatgacc
     1501 ttgacgagcc ggttgctgag gtacgagccg ttgccgagca ccgtgacgag cgcgtcgtac
     1561 accaccggct gggtcgtcgt gttctggcag aaatcgttct tgaacctgac atcgaaggtg
     1621 acggtggtcc cctgcttgac cgagaagaag gtggtcccgt ccttcccgct gataccgtcc
     1681 tcgggatcgg ccgcgacggt ggtcgacgac ttcacgaact cggcggcgct gatgccgtcg
     1741 cactcgtcat ccgacatctt gccggtcgtc acattcatat cgatgaaggt gatgaggtct
     1801 tttatcgcct gcgctatctg gtcgggcatc ccctcgccgg tcggcgtggc ggtgtggtag
     1861 atgaagggct tgccgtcctt gtcgaccgac ccggtgtact gcgcgagcat ctccatgtcg
     1921 ggctgggggt cgatgccggc gttcgcctcg ccgtcggccg tcttctcgtg ccaggtgttg
     1981 atgccgatga acttcgcgcc gatgccgccc atcaccgcga gcgcctcctc gaaggtgtgg
     2041 ccgttcatga aaccggcccc cgcgacccag tagcattcgg cgctcacctg caccccgttc
     2101 gggtcgcagt cctcccgctc ctcgtcggtt atctcgatgt atatcggcat cgatttcttc
     2161 cggaaacagg cgtggcccac cgtgccgatc tctccggtgc agttcgccgg ggcgatgtcg
     2221 accaccgcgc tggggatcat gttctgatta caaccgccca tcgcgggagg cagacagaat
     2281 ttcgcggtgc cgtggaactc ttcgcccgcc gcgagttgat agagcacctc gctgtgcagt
     2341 tcgttcgcgc cgaccgaggt gttgaacgcg tcctcgttga ccttgtccac cgccgatttc
     2401 accgcgtcga ccgaaaaggt ctgtttctgc cgcaggaact tcgtcgtgcc gttccaactg
     2461 atgcgcgaca cgccgaacga ggcgaacccc tcctgcggga actcggcctt gaccgggtcg
     2521 atgatgttgt cctttatgcc gttctgcacc tgcgctatct cgtcgttcat cgagccggag
     2581 atgtcgatga tgatctgcac atcgatcgcc tcgatgagcg tgctgaaggt gagcgtgcgg
     2641 gtcacctcct ccggcgcgtt gtagggcagc acgacgaaaa agagcccgtc gggtatcgtg
     2701 tcctcgtcgt cgagcgggtc ggagccgtac acgatctcgg cgaggtcgtc ggacccgtca
     2761 ccgtcgctgt ccttcttggt cgggctggtc ccttcctgca cctcctcctt gtccgacagg
     2821 ccgtcgttgt cgctgtcctt gtcgaggaag tcgggcgtat cgtcggcgtc gctgttcgcg
     2881 cagggctgtt cgggacactc cgtgccgtcg aggatgccgt cgccgtccga atcggggtcg
     2941 aggcagttgg gcgcgccgtc gaggtcgcga tcctcgctcc cgtcgacgcc gttggggatg
     3001 ccgtccgcat cctcgtcgcc gttcgggtcg gcgcacagtt ctaccaccgt gtcctcgtcg
     3061 gtgacgacgc ggctgcgctt ttcggggccc atctgtaccc gcg
//
LOCUS       DAOQUE010000350         3094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_36619_length_3094_cov_3.939454, whole genome shotgun sequence.
ACCESSION   DAOQUE010000350 DAOQUE010000000
VERSION     DAOQUE010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36619_length_3094_cov_3.939454"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..395)
                     /locus_tag="PKH10_10145"
     CDS             complement(66..395)
                     /locus_tag="PKH10_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT28524.1"
                     /translation="MSSVNGACQHPRSGRFLFRRGGAQGVDAARYWDRLYFWIDPKDI
                     YSGLIKAAVFAIIVTTISCFYGLTTRNGAKGVGISTTRAVVSSSVAILIADYVLADIL
                     ITMLYKN"
     gene            complement(278..841)
                     /locus_tag="PKH10_10150"
     CDS             complement(278..841)
                     /locus_tag="PKH10_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT28525.1"
                     /translation="MRFVTAYIGELLTVSGKYALFLLSTIGWTFRRPFRWKLLLEHME
                     KIGVNSLLIISLSALTVGMIFSLQVTYMMRIFRGETFVGAMVAMTMGRELAPVMTALM
                     LIAKNGSSMAAELGTMKVTEQIAAMETMAVNPIHYLVVPRMLASIVVYPLLTGLANIL
                     GVVGSYFVAVVLKGSTRHATGTGSISG"
     gene            complement(921..1715)
                     /locus_tag="PKH10_10155"
     CDS             complement(921..1715)
                     /locus_tag="PKH10_10155"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNT28526.1"
                     /translation="MRDIIVAAILGVIEGITEFLPVSSTGHLILANELFTFRDPVFAA
                     MFDIVIQSGAILSVLLYFRQRLVPIGDPAHRTRVLMLWRAAIVGVIPALVLGAALGDL
                     IEEHLFNPFVVAGSLLVGGIAILIVERRASPDGIATAEDIPLKIAFLIGLIQCLAMVP
                     GVSRSAATIIGAMLLGASRIAAAEFSFFLAIPTLLAATAYSLLKHSALVTGEQATALA
                     VGFSVSFLTAYAVIALFMRWISTRTFAPFGWYRIALAAAVVAYFAA"
     gene            1892..2128
                     /locus_tag="PKH10_10160"
     CDS             1892..2128
                     /locus_tag="PKH10_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013712407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNT28527.1"
                     /translation="MKEFIAEIAKHLVDRPDEVEVNFIEGKSTAIIELRVAREDVGKV
                     IGKSGRTANALRTILSAVASRHGQKVVLEIIEEK"
     gene            2136..>3094
                     /locus_tag="PKH10_10165"
     CDS             2136..>3094
                     /locus_tag="PKH10_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28528.1"
                     /translation="MTAGMRIRAASRCGGGFSFLPFTLLLSLLAFGAVLFVILRNYGA
                     FDDADRLAGAHRYAESLKEFFVPEPVKYKVYDIPILVRAQDRGSEFWNVTFPNFLRAA
                     REETDRILAYERAINKRDDNGDHAAVNIADEPMLIAILKEGKDLSGRTLFNIAAGDIF
                     DFWDTAARRYDNWVLAREHHFATTQLLEMKKNELTEEERKKHTEELARLERILAKDRF
                     LPDPKAVAGFLPHAKPAAIEIDEQGGTVRVTDPNAAVAISMLREAIFLERLRAKLPKD
                     LHYLIYFGDRHGIWKLPAKYARWTVSVDDPLVVLGDKSLGAVRL"
BASE COUNT          682 a          895 c          978 g          539 t
ORIGIN      
        1 gcaggacctg ttgttcgtaa aatgacttgt ggagatcccg tatgtcgatg atgcggtcgg
       61 tcatgtcagt tcttgtacag catggttatc agtatgtcgg cgagcacata gtcggctatc
      121 agtatcgcca ccgaactcga cacgacggcg cgggtggtcg agatgccgac ccccttggct
      181 ccgttgcggg tcgtcagtcc gtagaagcac gatatcgtcg tcacgatgat cgcgaagacc
      241 gccgccttga tgagccccga atagatatcc ttcgggtcta tccagaaata gagccggtcc
      301 cagtagcgtg ccgcgtcgac cccttgagca ccaccgcgac gaaataggaa ccgaccactc
      361 cgaggatgtt ggcaagcccc gttaacagag gatataccac gatcgaggcg agcatccgtg
      421 gcacgacgag atagtgtata gggttgaccg ccatcgtctc catcgccgct atctgctcgg
      481 tcaccttcat cgtccccagt tcggccgcca tcgatgagcc gttcttcgcg atgagcatga
      541 gcgcggtcat gaccggcgcc agttcgcggc ccatcgtcat cgccaccatc gccccgacaa
      601 aggtctcgcc gcggaagatg cgcatcatgt aggtgacctg cagggagaat atcatgccga
      661 cggtgagtgc cgacagcgat atgatgagaa gcgagttgac acctatcttc tccatgtgtt
      721 cgagcaggag tttccaccgg aaggggcggc ggaaggtcca gccgatggtc gagaggagaa
      781 aaagggcgta tttgcccgaa acggtaagca gttcgccgat gtaggcggtc acgaaacgca
      841 tgtcaggtcg tccattctcc ctagaaaggc acgcgaacac taccggaaag aggcgcgata
      901 gtcaacaatt atacgggatg ttatgccgcg aagtaggcga ccaccgcggc ggcaagggcg
      961 atgcgatacc agccgaacgg ggcgaaggtg cgggtcgata tccagcgcat gaagagggcg
     1021 atgacggcgt aggcggtgag gaacgaaacg gaaaaaccga ccgcgagcgc cgtcgcctgt
     1081 tcgccggtga ccagcgccga atgtttgagc agactgtagg cggtcgccgc aagaagggtg
     1141 gggatggcga ggaagaacga gaactcggcc gccgcgatgc gggaggcccc caacaacatc
     1201 gccccgatga tggtcgccgc cgaccgcgag acgccgggga ccatcgcgag gcactggatg
     1261 agcccgatga ggaacgcgat cttcagcggg atgtcctcgg ccgtcgcgat gccgtcgggc
     1321 gaagcgcgcc gctcaacgat aagtatcgcg atgccgccga ccagcaacga cccggcgacg
     1381 acgaacgggt tgaagaggtg ttcctcgatg aggtcgccca gcgccgcgcc gagcacgagc
     1441 gccgggatca ctccgacgat ggccgcccgc cagagcatca ggacgcgggt ccggtgggcc
     1501 gggtcgccga tcgggacgag ccgctgccgg aaataaagga ggaccgacag gatggcgccc
     1561 gactgtatca cgatgtcgaa catcgcggcg aagaccgggt ccctgaaggt gaagagctcg
     1621 ttggcgagta tgaggtgacc ggtcgaggaa acggggagga actccgttat cccctcgatg
     1681 acgcccagta tcgccgcgac gatgatgtcc cgcatggtcc gccctgcctc cttggttcag
     1741 acgcgactat ggcaaaaagg cggtcaggag tcaaaaatta tattgaaaaa cggttcccga
     1801 tggatacaat caacctttat agttgttcgt attgtaacgg aacatcgcag gaacgaacgg
     1861 taacagcaac ggtcattgag ggaggaacga gatgaaggag ttcatcgcag agatcgccaa
     1921 acacctggtc gatcgtcccg acgaggtcga ggtcaatttc atcgagggga aaagcaccgc
     1981 catcatcgag ttgcgcgtgg cgcgcgagga tgtcggcaag gtcatcggga aaagtggccg
     2041 caccgccaac gcgttgcgga ccatcctttc ggcggtcgcc agccgccacg ggcagaaggt
     2101 ggtcctcgag atcatcgaag agaagtgacc gcgccatgac cgctggaatg cgcatcaggg
     2161 ccgcctcccg ttgcgggggc ggcttttctt ttcttccatt caccctcctt ctgtccctgc
     2221 tggccttcgg cgccgtgctc ttcgtcatat tgcgtaacta tggcgcgttc gatgatgccg
     2281 accgtctggc gggggctcac cggtacgccg aatcgcttaa ggagttcttc gtccccgaac
     2341 cggtcaaata caaggtctac gatatcccga tactcgtccg ggcgcaggat cgcgggtccg
     2401 aattctggaa cgtgaccttc ccgaacttcc tgcgcgcggc gcgcgaagag accgaccgga
     2461 tactcgctta cgaacgggcc atcaacaaac gcgacgacaa cggcgaccac gcggcggtga
     2521 acatcgccga cgagccgatg ctcatcgcga tactcaagga ggggaaggat ctctccggca
     2581 gaacactttt caatatcgcg gcgggcgaca tattcgattt ctgggatacc gcggcgcgtc
     2641 gctacgataa ctgggtgttg gcgcgggagc accacttcgc gacgacgcaa ctcctcgaga
     2701 tgaaaaagaa cgaactgacc gaagaggaac ggaagaagca taccgaggag ttggcgcggc
     2761 tcgaacggat actggccaag gaccggttcc tgcccgaccc gaaggcggtc gccgggttcc
     2821 ttccgcacgc gaagcccgcg gcgatagaga tcgatgagca gggcggcacc gtccgcgtca
     2881 ccgatcccaa cgcggcggtt gccatatcga tgctccgcga ggcgatattc ctcgagcggc
     2941 tgcgcgccaa actgcccaag gaccttcact atctcatcta tttcggcgac cgtcatggca
     3001 tctggaaact gccggctaaa tatgcccgtt ggacggtgag tgtagacgac ccgctcgtgg
     3061 tgcttggcga taagtcgctc ggcgcggtgc ggct
//
LOCUS       DAOQUE010000351         3083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_36831_length_3083_cov_4.048547, whole genome shotgun sequence.
ACCESSION   DAOQUE010000351 DAOQUE010000000
VERSION     DAOQUE010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36831_length_3083_cov_4.048547"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKH10_10170"
     CDS             complement(<1..326)
                     /locus_tag="PKH10_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28529.1"
                     /translation="MSGYFQGRNSYKIDAKGRFPFPAHWFPPLDLKRDETLIAARGMS
                     PDAKFLELYGPAAWAERMALIERAFPQGALRDQFIRWYVSTAEKVELDTQNRIRIPRH
                     LMEYAG"
     gene            604..1512
                     /locus_tag="PKH10_10175"
     CDS             604..1512
                     /locus_tag="PKH10_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28530.1"
                     /translation="MRPLLVPLAVLVLFACTEQGPQKIAAPETTAVCAGDCVRGQFSP
                     DGATVAYTGAKYRGLSLHSLRDGKTTLLSDVGGAGIGPVFSPDGAAVAFRERYDTADG
                     TRERIVTVKVADLSRSVEADGLTVTMPLFWRGALTAVSPDTLRPTIFHGTDAKASAAL
                     PTAVTTANGVRILPAPDAKPVTLAAGGHTPTVSPDGRWVAYIKGNAVRLYDLASGSDR
                     RLADGSHPVWMPDGRGLIVAATRDDGRDIVGATLSLVSLDGAARRIMTPSEAVPLYPD
                     VTADGARILYTDHATGRILVQSLVLE"
     gene            1529..>3083
                     /locus_tag="PKH10_10180"
     CDS             1529..>3083
                     /locus_tag="PKH10_10180"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT28531.1"
                     /translation="MKKHIAFLFLVLCSFALDASLSGKKICIDPGHGGTDPGALGVNG
                     STYPDEADLVLDIDLRFRTLLTADDASVLMTRDADTTVSLANRVDQANAWGATIFLAT
                     HLNSADLASASGTETFAYQSGGTSASLATTVQDELIDHMDRVDRGVKYESFYVIKYTD
                     MPAILSEGVFVSNSGDFTYITSGEGKEAHAVALYHAVCSHFGVTPQDPGSGGDEPGAV
                     KGFVYDLTTGLGNVEGNRIADATVTITPTSGSPSTLTTPATGLFSFTGVEPGDYTLSV
                     EKSGFVTAQKDITVTSNTDTWASTGIEEESAQATATVKGFVYDLSTGLGNIEENRIAD
                     ATVTVKNNGTNAEATVESSATGYFTFTGLEAGSYTLTVVKSGYQNASRTLDLVAGDNW
                     ASTGIEKESGVTTGSVTGSVYALTMLLNPSGIAGATVTLTPAGGGADEVATTDANGDF
                     SFTAITAGDHTLTAEASGYQTAQKDITVVAAQTLEIGIGLSPVGGSDDGSPNDDEITT
                     DGMPLPDDLL"
BASE COUNT          572 a         1075 c          942 g          494 t
ORIGIN      
        1 atgcccgcgt actccatgag gtggcgcggg atgcggatgc gattctgcgt gtcgagttcc
       61 accttctcgg cggtcgaaac ataccagcgg atgaattggt cgcgcaatgc cccctgcggg
      121 aaggcccgtt cgatgagcgc catccgctcg gcccacgccg cgggtccgta cagttcgagg
      181 aacttcgcgt cggggctcat gccgcgcgcc gcgatgagcg tttcgtcgcg cttgagatcg
      241 agcggcggga accagtgggc cgggaacggg aaccgccctt tcgcgtctat cttataggaa
      301 ttacgcccct gaaagtaacc ggacacgctc ttttgtctcc acttgatgac acttcgaata
      361 caaaatttac caccagtcac ttggaaagca agaaaaaaat aaaaaaagtt ggtcgctaaa
      421 gaatcgaatt gccaatgatg tagtttttgc ggaaaagcgg aggcgacgat cggagatctg
      481 gatccctcgc ctgctatcca ccttgtcgag agatcgacgc ggtacggaga aaaactttac
      541 ttgccgcccc ttcccgtgct tcaatacggc ggaactgaca ccatgatgac aagggaggat
      601 cccatgagac cgttgcttgt cccgcttgcc gttcttgtgc tgttcgcctg caccgaacag
      661 ggcccgcaga agatcgcggc gcccgagacg accgccgtct gcgccggcga ctgcgtgcgc
      721 ggccagttct cgcccgacgg cgcgacggtc gcctacaccg gcgccaaata ccgcgggctt
      781 tcgctccatt ccctgcgcga cgggaagacg acgcttcttt ccgacgtcgg cggcgcgggc
      841 atcggcccgg tcttctcgcc cgacggcgcg gccgtcgcgt tccgcgagag atacgacacc
      901 gccgacggga cccgcgaacg gatcgtgacg gtgaaggtcg ccgacctttc ccgctcggtc
      961 gaggccgacg ggctgaccgt tacgatgccg ctcttctggc gcggcgcgct cacggcggtg
     1021 agccccgaca cgctcaggcc gacgatcttc cacggcaccg acgccaaggc gagcgcggcc
     1081 ctgccgaccg ccgtgacgac cgcgaacggc gtgcgcatac tccccgcccc cgacgcgaag
     1141 ccggtgacgc tcgcggcggg cggtcacacc ccgaccgtat cgcccgacgg ccgctgggtc
     1201 gcctacatca aaggcaacgc ggtgcggctg tacgatctcg cgagcggctc ggaccgccgg
     1261 ctcgccgacg ggtcgcaccc ggtctggatg cccgacgggc gcgggctcat cgtcgccgcg
     1321 acgcgcgacg acggccgcga catcgtcggc gcgacgctct ctctcgtgtc gctcgacggc
     1381 gcggcgcgcc gcatcatgac cccgtccgaa gcggtgccgc tctaccccga tgtgaccgcc
     1441 gacggtgcgc ggatactcta caccgaccac gcgacgggac gcatcctcgt ccagtcgctc
     1501 gtgcttgaat aggtctaggg aggcggccat gaaaaaacat atcgctttcc tgttccttgt
     1561 tctttgttcc tttgcgcttg acgcatcgct ttccggcaag aagatctgca tcgatccagg
     1621 gcatggcggc accgaccccg gcgcgctcgg cgtgaacggc tcgacctatc ccgacgaggc
     1681 cgacctcgtg ctcgacatcg acctgcggtt ccgcaccctg ctgaccgccg acgacgcgtc
     1741 ggtcctcatg acgcgcgacg ccgacacgac cgtttcgctc gccaaccgcg tcgatcaggc
     1801 caacgcgtgg ggcgcgacga tattccttgc gacccacctg aactcggccg acctcgcgag
     1861 cgcgagcggc accgagacct tcgcctacca gtcgggcggc acgagcgcct cgctcgcgac
     1921 cacggtgcag gatgaactca tcgaccacat ggaccgcgtc gaccgtggcg tcaagtacga
     1981 gagtttctat gtcatcaaat acaccgacat gccggcgata ctctccgaag gggtcttcgt
     2041 gtcgaactcc ggcgacttta cctacatcac ctccggcgag ggcaaggagg ctcacgcggt
     2101 ggcgctctac cacgccgtct gctcgcactt cggcgtcacc ccgcaggacc cgggaagcgg
     2161 cggcgatgaa cccggcgcgg tcaaggggtt cgtctatgac ctcacgaccg gtctcggcaa
     2221 tgtcgaaggc aaccgcatcg ccgacgcgac cgtcaccatc accccgacga gcggctcccc
     2281 ctcaacgctc accacccccg cgaccggcct gttctctttc accggcgtcg agccgggcga
     2341 ctacacgctg agcgtcgaga agagcggctt tgtgaccgcg cagaaggata tcaccgtcac
     2401 gagcaacacc gacacctggg cctccaccgg catagaggaa gaatcggcgc aggccaccgc
     2461 aacggtcaag ggcttcgtgt acgacctctc gacgggactc ggcaacatag aagagaaccg
     2521 catcgccgac gcgaccgtca ccgtcaagaa caacggaacg aacgccgagg cgaccgtcga
     2581 gagttccgcc accggctact tcaccttcac cgggctcgag gcgggcagtt acaccctgac
     2641 cgtcgtgaag agcggctacc agaacgcctc gcggacgctc gacctcgtcg cgggcgacaa
     2701 ctgggcctcg accggcatcg agaaagagag cggcgtgacg accggctcgg tgaccggctc
     2761 ggtctacgcc ctcacgatgc tcctcaaccc atcgggcatc gccggggcga ccgtgacgct
     2821 gacccccgcg ggcggcggcg ccgatgaggt cgcgaccacc gacgcgaacg gcgacttctc
     2881 tttcaccgct atcacggcgg gagatcacac actcaccgcg gaggcatccg gctaccagac
     2941 ggcgcagaag gatatcacgg tcgtcgcggc ccagacgctc gagataggga tcggtctctc
     3001 gccggtcggc ggctccgacg atggatcgcc gaacgatgac gagatcacga ccgacgggat
     3061 gcccctgccc gacgacctcc ttg
//
LOCUS       DAOQUE010000352         3080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_36887_length_3080_cov_4.702810, whole genome shotgun sequence.
ACCESSION   DAOQUE010000352 DAOQUE010000000
VERSION     DAOQUE010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36887_length_3080_cov_4.702810"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PKH10_10185"
     CDS             <1..678
                     /locus_tag="PKH10_10185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HNT28532.1"
                     /translation="GFYVEIGGGAGSLTEKAVGRGLTPFTVLDIDETMLQILHTRFDG
                     KAQVINADAVRYDLTPHFAGTRGVVFGNLPYQASSPILLSVLSQSRYISRAVFLLQKE
                     VAVKCAAEPASRDFSPLAAVIRHIGDAEILFDIGPASFFPPPKVMSSVLQIPIRSHDK
                     TPAEIMKMADAFRILFSHRRKTLANVFKMHRLPAELLAEFSIGATARIEEIDWPVLEA
                     LAARLAG"
     gene            complement(717..1181)
                     /locus_tag="PKH10_10190"
     CDS             complement(717..1181)
                     /locus_tag="PKH10_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNT28533.1"
                     /translation="MPKPQKEFKELFTSLNARGVEYLVVGAYAMAFHGVPRFTGDVDV
                     LVRPTKENAKRLIAALDDFGFGSLGLTIADFSDPDQVVQLGVAPLRVDILTSISGVSW
                     EQAILNKCDTTYGKVPVPFLGLNEIIANKRAVGRMKDMADIELLESVTKKRN"
     gene            complement(1120..1308)
                     /locus_tag="PKH10_10195"
     CDS             complement(1120..1308)
                     /locus_tag="PKH10_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28534.1"
                     /translation="MIERSVNKHKLGAIDEQKADLLFWLSKTPAERVAAVDFLRAQHA
                     ETSKRIQRTVHIVKRAGR"
     gene            1307..3001
                     /locus_tag="PKH10_10200"
     CDS             1307..3001
                     /locus_tag="PKH10_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28535.1"
                     /translation="MKGIYRYPARLSSAFRRATGFFCPPLYFHFPFGYTCDMVVKQYI
                     IIAFAALLVACEPGVQGSRSDMAPVDSDWAVTDDAGGLEEESGILDEDVVSDETLEDE
                     DIAPDTDNHIGPGDKEPVEVDDDPWAGMLERVPFVWYNTKPEQSSDPDGIKELWYQLR
                     IEERWPLSECTPKIYDQCAENYPHEAVTRAPCGEAISANLDPKILQNSYDYILTPYCW
                     GTYPMEFPLYSLKGSCLSFYSSTASPYDAGDFVYDLDSRKLIRIGRGLAIPPFSNGEY
                     FFGGTTDFSTVWEEEYEEVSYYYHFARKEYGKTWKMPQKLSYVRDIVASDQYMAMNLV
                     FLTGNRNEPLRGYYTKIGEWDKWVELNFGNFPSYGIGRPEIQGNYLVSYNYFDNLLRV
                     FFCDLAKGESSCRIVSKTGEIARKPIWGTKNTFYYSVETGSEGATIYQATVDDTGDQE
                     TTVTSASLFSSDKYMQVHQVNEEYLLYLKYIKEGSPININNPCYYRFLDKKHFCLEDG
                     INSDIAYQEARFYDKWMVFQSNRDLILRDMDGYCKEHSANCPFEDIKTTTIKGDGK"
BASE COUNT          743 a          789 c          819 g          729 t
ORIGIN      
        1 gggttctatg tcgagatcgg cggcggggcg ggatcgctca ccgaaaaggc ggtggggcgc
       61 gggctcaccc cgttcacggt actcgacatc gacgaaacga tgctccagat attgcatacg
      121 cgattcgacg gtaaagcgca ggtcatcaat gccgacgcgg tccgctacga tctgacgccg
      181 catttcgccg ggacgcgcgg cgtcgtgttc ggcaacctgc cctatcaggc gagttcgccg
      241 atactgttgt cggtcttgtc gcagagccgt tatatctcgc gggcggtgtt cctcctgcag
      301 aaagaggtgg cggtgaagtg cgcggccgag ccggccagcc gcgatttttc gccgctcgcc
      361 gccgtcatcc ggcacatcgg cgacgccgag atactcttcg acatcggccc cgcgtcgttc
      421 ttcccgccgc ccaaggtgat gtcgtcggtg ctccagatac cgatacggtc gcacgataaa
      481 acgcccgcgg agatcatgaa gatggccgac gccttccgca tcctcttctc gcaccgacga
      541 aagacgcttg ccaatgtctt caagatgcac cggcttccgg ccgaactgct tgccgagttc
      601 tcgataggcg cgaccgcccg tatcgaggag atcgactggc ccgtcctcga agcgctcgcc
      661 gcgcggttgg cgggataatt cttaaaaggt atccggatcg aaagacgata ctatttctag
      721 tttcgtttct tggtgaccga ttcgagtagc tcgatgtcgg ccatatcttt catgcggccg
      781 acggcccgtt tattcgcgat gatctcatta agaccgagga agggaacggg gactttgccg
      841 taggtcgtgt cgcatttgtt caggatagcc tgttcccacg aaacgccgga aatggaggtg
      901 agaatgtcca cccgaagcgg cgcaacaccg agttgcacca cctgatcggg atcactgaaa
      961 tcggcgatcg tcagaccaag cgacccgaaa ccgaaatcat cgagcgccgc aatgagccgc
     1021 ttcgcattct ctttcgtcgg ccggacaagg acatcgacat cgccggtgaa gcgcggtacg
     1081 ccgtgaaagg ccatcgcata ggccccgacg acgagatact caacgccccg cgcgtttaac
     1141 gatgtgaaca gttctttgaa ttctttttga ggtttcggca tgctgtgctc gtaaaaaatc
     1201 gacggcggct acccgttcgg cgggtgtttt tgaaagccag aaaagcaggt cggccttctg
     1261 ttcatcaatc gcgccgagtt tgtgtttgtt gaccgatctc tcaatcatga agggaatata
     1321 tcgttatcct gcgcgattgt caagcgcatt tcgccgtgca acgggcttct tttgtcctcc
     1381 actttacttt cattttccgt tcggctacac ttgcgacatg gttgtgaaac aatacatcat
     1441 catagctttc gcggcacttc ttgtcgcctg cgaaccaggg gtgcagggaa gccgttccga
     1501 catggcgcct gtcgatagtg attgggcggt aactgatgat gcaggggggc tggaagaaga
     1561 atccggcatc ctcgacgaag atgtggtcag cgatgaaaca ttggaagatg aagatattgc
     1621 gcccgatacc gacaaccaca tcggtcccgg tgacaaggag ccggtcgagg tggatgacga
     1681 tccgtgggct ggcatgttgg aacgggtgcc gttcgtatgg tacaacacga aaccggaaca
     1741 gtcatccgat ccagacggga taaaagaact ttggtatcaa ttgagaatag aggaacgatg
     1801 gcctctttcc gaatgtacgc cgaagatata tgaccagtgc gcggagaact atccgcacga
     1861 agcggtgacc cgcgcaccct gtggagaagc gatcagcgcc aatcttgatc ccaaaatact
     1921 ccagaactcc tacgattata ttcttacgcc gtattgttgg ggaacatacc ccatggagtt
     1981 ccctctttac tccttgaaag ggtcatgtct ttctttttat tccagcaccg cttctccgta
     2041 tgatgccggt gattttgtat atgatcttga ttccagaaaa ctcatacgca ttggtcgagg
     2101 attggcaatt cctccatttt caaatggaga gtatttcttt ggcggcacca ccgatttctc
     2161 gacggtatgg gaagaagagt atgaagaagt ttcctattat tatcatttcg ccagaaaaga
     2221 atacggcaaa acatggaaga tgccgcagaa actttcgtat gtgcgagaca ttgtcgccag
     2281 cgaccagtat atggccatga atctcgtgtt tctcacgggc aatcgcaatg agcccctgcg
     2341 cggatactat accaagatcg gcgagtggga caagtgggtc gaattgaact tcggaaactt
     2401 cccttcctat ggtatcgggc gtccggagat acagggaaat tatttggtct cttataacta
     2461 tttcgacaat ctcttgcgcg tgtttttctg cgatctcgcc aaaggcgaat cgagttgcag
     2521 gatcgtgagc aaaacgggcg agatcgcccg taaaccgata tgggggacca aaaacacttt
     2581 ttactactcg gttgagacgg gaagcgaggg ggcgactatt tatcaggcaa ccgtggatga
     2641 taccggagac caagaaacaa cggtgacatc ggcttctctt ttctcatcag acaaatatat
     2701 gcaggtccac caagtaaacg aggagtatct tctatacctt aagtatataa aagagggtag
     2761 ccccatcaat ataaacaatc cttgctatta tcgttttttg gacaaaaaac atttctgtct
     2821 ggaagacggc attaacagcg atatcgctta tcaggaagca cgtttttacg acaaatggat
     2881 ggttttccaa tccaacagag acctgatcct gagagacatg gacggctact gcaaagaaca
     2941 ctcggcgaac tgtccgtttg aagatatcaa aacaacaacc atcaagggag acgggaaatg
     3001 aacagagcga aaaccatcgg tacgatgctt tttcttgcaa ttcttttgtc tttcactccg
     3061 attcatgcgg aattgatctc
//
LOCUS       DAOQUE010000353         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_37047_length_3071_cov_7.559682, whole genome shotgun sequence.
ACCESSION   DAOQUE010000353 DAOQUE010000000
VERSION     DAOQUE010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37047_length_3071_cov_7.559682"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PKH10_10205"
     CDS             <1..383
                     /locus_tag="PKH10_10205"
                     /inference="COORDINATES: protein motif:HMM:NF025815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28536.1"
                     /translation="LPSLVFDLTDRSLSVYAVAGAAVTPRSLVKNVPLPNIYEDGRIC
                     MGNCKLPAMRGDIADLIERYEALFYRSKFTHFIHKEPTRSPVTALFKALAGSDAFPVE
                     ELTPLNGRSITVQQLVNHDRRKTA"
     gene            380..1180
                     /locus_tag="PKH10_10210"
     CDS             380..1180
                     /locus_tag="PKH10_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system ThiF family protein"
                     /protein_id="HNT28537.1"
                     /translation="MNPFHATHEALIRAVHPVSVSLIGCGGTGSQLLTNLARMDAALR
                     ALGHEGLQVKVFDPDTVTAANVARQSFPASEIGAFKAVSHTQRVNRFFGLRWEAYPEA
                     VKASEPHPAFRSHVYLSAVDSVVARIEILNAIRRTRQDHHYRDTPLYWIDCGNTKTTG
                     QVVIASLFTRPKEKKNRHLKNIFELYPDYERADKDDDSPSCSLAEAIGKQDLYINTMM
                     AAYAADLLWKLIRQAGVDCQGIFVNLDSLQTRPIPLHQPKTRIRRKRA"
     gene            complement(1319..2878)
                     /locus_tag="PKH10_10215"
     CDS             complement(1319..2878)
                     /locus_tag="PKH10_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28538.1"
                     /translation="MKRIFATGGGLAAPIFMLFLLFVGACNTSKQTQDADTVNPDTET
                     IVVVDDTVFIEGESDTIDDTLISDTDVADCPSLASAPFPYYKEDGTIHFCRGCDTPTE
                     KDPQCVRNLWDAQNKKLTKDYPQYDCYPYPCDMPNLKPMTKDDVYAYYETIAMHECDV
                     LLTPKGWTADGTLGSVKHFNLSEGKVGFMMDAVDINVTQGYSTDFKVFEYDVATKNYH
                     VVGPIYLYGLAYYKGKALTFMTDFRKDLDNIDVIQSYVGYFGSDGAYRVVYNKPINYI
                     AYTPVMNDRWVLANIQEVIDGPYFVKYAKVGEWKWTNLMAGQGRKPDIQEDMISLHFF
                     DEPSGIMKGYICDLSTSPKSIGDCQLVNQEGESVYHIQFDKSGAKRFVYSSIFQKSVV
                     LVDYSQGPGKWVRSDLITEFDDTMKNAYLMAPDQFRGDLLLYREGADMGGGAFSVLAC
                     YYRLDKKKKYCMKKMDRDFVYDDGTIRYPYGDSEFEGDYFLYQKSNSTPFILRDMKCY
                     CEKEGICPFEE"
     gene            complement(2881..>3071)
                     /locus_tag="PKH10_10220"
     CDS             complement(2881..>3071)
                     /locus_tag="PKH10_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28539.1"
                     /translation="KVSTNNEVPSYCGNGIVEPGEVCDGNSVYCEDLDPNEWNSGTAY
                     CNSTCTGYDTGDCYWSGC"
BASE COUNT          710 a          925 c          724 g          712 t
ORIGIN      
        1 ccctgccgtc gctggtgttc gacctcacgg accggtcgct ttcggtttat gccgttgcag
       61 gcgccgccgt aacaccccgg tcactggtca agaatgtacc gctccccaat atctatgagg
      121 acggccggat ctgcatgggg aactgtaaac tccccgcgat gcgaggcgat atcgccgatc
      181 tcatcgaacg gtatgaagcg ctcttttacc gctcgaagtt cacccatttc atacataagg
      241 agccgacccg gagtccggtg accgcccttt ttaaggcgct cgccggaagc gacgccttcc
      301 cggtggagga actcactccg ctcaacgggc ggagcatcac cgtacaacaa ctcgtcaacc
      361 acgacaggag gaaaaccgca tgaacccctt tcacgcgacg cacgaagcgc tcatccgcgc
      421 cgtccatccc gtttcggtat cgctcatcgg ttgcggcggt accggctccc aactgctcac
      481 caacctcgcc cgcatggacg cggccctccg cgccctcggc catgaaggcc tgcaggtcaa
      541 ggtattcgac cccgacaccg tgacggccgc caatgtcgcc cgtcaatcgt tccccgcctc
      601 cgagataggc gccttcaagg cggtctccca cacccagcgg gtgaaccgct tcttcgggct
      661 ccggtgggag gcctaccccg aggcggtcaa ggcatcagaa ccgcaccccg ctttccgctc
      721 ccatgtctac ctttcggcgg tcgactcggt cgtcgcccgc atcgagatac tgaacgcgat
      781 acgccggacc cggcaggacc accactaccg cgacacgccg ctctactgga tagactgcgg
      841 caacaccaag acgacggggc aggtggtcat cgcgtcactc ttcacccgcc ccaaggaaaa
      901 gaagaaccgg cacctgaaga acatctttga actctacccc gactacgaac gggccgacaa
      961 ggacgacgac tccccctcct gctcactcgc cgaagcgatc ggcaagcagg acctctacat
     1021 caacacgatg atggcggcct acgccgccga cctcctctgg aaacttatcc ggcaggccgg
     1081 cgttgactgt caggggatat tcgtcaacct cgactcgctc cagacgcggc cgataccgct
     1141 ccatcaaccc aagaccagaa tacggaggaa aagagcatga gatggagacc cgccgtcccc
     1201 tcctgcggag gacgcgggtg gcaaatgaag gcgaataccc ccgcgaatga cattttattc
     1261 ttttgggcag ttgggtccat tatgcttttt ccgcttgatc cagacactct taggaaaatt
     1321 attcctcgaa cggacagatg ccttctttct cgcagtagca tttcatgtca cgcaggatga
     1381 agggcgtact attactcttt tggtataaga agtagtcacc ttcgaactcc gagtctccgt
     1441 atgggtaacg aatggtgccg tcgtcataaa caaagtcccg gtccatcttt ttcatgcaat
     1501 acttcttctt cttatcaagt ctgtaatagc acgctaggac actaaaagct cctcctccca
     1561 tatcggcgcc ttctcgataa agcaaaagat cgcccctaaa ctggtcgggg gccataagat
     1621 aggcgttctt catcgtgtcg tcgaattccg ttatcaagtc gctacgaacc cacttccccg
     1681 gtccctgact gtaatcgaca agcacgaccg acttctggaa tatcgaactg tagacgaatc
     1741 gcttggcgcc gctcttatcg aattgtatgt gatagacact ttccccttct tggttgacca
     1801 gttggcaatc accgatagat tttggggaag ttgaaagatc gcaaatatac cctttcataa
     1861 taccggacgg ttcgtcaaaa aaatgtagag atatcatgtc ttcctgaatg tctggttttc
     1921 gcccttggcc agccatcaag ttcgtccatt tccattcacc gaccttcgca tatttcacaa
     1981 aatacgggcc gtctatcact tcctgtatgt tcgccaaaac ccatcgatca ttcatcaccg
     2041 gcgtgtaggc aatatagttt attggcttgt tgtataccac acggtaggca ccatcgctcc
     2101 caaaataccc gacataactt tgtatcacat caatgttgtc aagatccttt ctgaaatcgg
     2161 tcatgaaagt gagtgctttc cccttatagt aggctaggcc atatagataa atggggccaa
     2221 caacatgata gttcttagtg gccacatcgt actcgaacac cttgaaatcc gttgaataac
     2281 cttgtgtgac atttatatcg acagcatcca tcatgaatcc gactttacct tcagacaggt
     2341 taaagtgttt caccgagcca agagtaccat cggcagtcca acccttgggt gtcaacagaa
     2401 catcacattc atgcatagct atagtttcat agtacgcata aacatcatct ttggtcattg
     2461 gtttcaggtt cggcatatcg cacggataag gatagcaatc gtactgagga tagtccttgg
     2521 taagtttctt gttctgtgcg tcccagaggt tccggacgca ttgcggatct ttttccgtcg
     2581 gtgtatcaca ccctcggcag aaatggatcg tgccatcctc cttgtaataa gggaacggag
     2641 cgcttgcaag cgacggacaa tcggcaacat cagtatcaga gatcagcgtg tcatcgatag
     2701 tgtcgctctc tccttcgatg aatacggtat cgtccaccac tactatcgtt tcggtgtccg
     2761 ggttcacggt atcggcgtcc tgtgtctgct tcgaagtgtt gcaggccccg acgaaaagca
     2821 agaagagcat gaatatgggg gcggccaagc cgcccccggt agcaaatatc cgtttcatcc
     2881 tcagcaccca ctccagtaac agtctcccgt atcataaccg gtgcaggtcg aattgcaata
     2941 ggccgtaccg ctattccact cgttcgggtc gagatcctcg cagtagacgc tgttcccgtc
     3001 gcatacctcg cccggttcca cgatcccgtt cccgcagtaa ctcggcacct cgttgttcgt
     3061 cgagaccttc a
//
LOCUS       DAOQUE010000354         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_37056_length_3071_cov_3.826260, whole genome shotgun sequence.
ACCESSION   DAOQUE010000354 DAOQUE010000000
VERSION     DAOQUE010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37056_length_3071_cov_3.826260"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..792)
                     /locus_tag="PKH10_10225"
     CDS             complement(103..792)
                     /locus_tag="PKH10_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT28540.1"
                     /translation="MEHVIRIDRVSKIYRTGEVTVEALKEVSLTIAKGEFSAIAGPSG
                     SGKSTLLNIVSGLDKPDGGTVELGGKAMTAMSASDLSDFRRDHIGFIFQSYNLIPVLT
                     VEENIEYVMLLQGVPKAERRERVAKILTEVGLDGMQTRLPIHLSGGQQQRVAVARAIV
                     SRPDIILADEPTANLDSKTADALLDMMQELNRTHGVTFLFSTHDQMIMDHARRLITLR
                     DGRIFSDEVRG"
     gene            complement(809..2032)
                     /locus_tag="PKH10_10230"
     CDS             complement(809..2032)
                     /locus_tag="PKH10_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT28541.1"
                     /translation="MMLFVKLAWRNLFRNKRRTILAGLMIGLGLAAMITTDAVMKGTI
                     DNMIATATSSFLGEGQIHRDGFRKTFEVEQTIVNGPALFDKLKKEPLIRRASPRTASF
                     GMITSPANVQSVMLYGIDPADEKELSKIDDLLVSGAYLATADEAKILLGEKTADLLEV
                     GLGDKIVVTVAQAHTGELSQELFRVGGIFRFGSRQMDGGMAFIPLKRSQEMLKLDGGL
                     HEIAFSFHDNLKSEDTALPLWSELSAGGNEALSWTELVSELAMMRSMTDFSLYLVGII
                     LFGIVAVGIVNTLFMSLYERMFEFGVMRAVGTRPRQAAQLMVMEAGALALIAIAMGIV
                     IAFIVNGLLMHYGIDYSGSEFVVTLRDPIYPVLTVTQFTLYPLLLFIFTCLVGLYPAI
                     YAARLTPAKAMKRGL"
     gene            complement(2042..>3071)
                     /locus_tag="PKH10_10235"
     CDS             complement(2042..>3071)
                     /locus_tag="PKH10_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNT28542.1"
                     /translation="DRPSLYKNFADTPELYAAITGDPQVRAATPRIHSSALAFVETKT
                     TGVKLIGIDPDREKRTTRLAAKVSEGAFLPATAAKQALIGRNVADVLKAKVGSELVLI
                     SQGADGSVANDLFTVGGILGDAKDTSEQNNVYLHITDAQDYLMLGGKIHEIAVLFADH
                     KKAEKMTERLGATLATAGFRDLSAESWMEVEPEFYRTMQADRKGDNISQFIIMLIVAV
                     GVLNTVLMTVLERTREYGVMAAIGTSPRRIFSLIVIETAMLALASCLIGAFLASFALW
                     YFVAVGISYPEPVTIAGLTLTTLRSEFSAIAYYKPLLIVVLTAVLVSVLPAIRAARIR
                     PIDALRSL"
BASE COUNT          548 a          921 c          991 g          611 t
ORIGIN      
        1 ctagatactg tttcccccag cccgaaagcg tcagcccttc ggcaagttcg accgcgccga
       61 gtgtcgcgtt gaaaagcagt atgaggacga agatcctccg catcagccgc gcacctcgtc
      121 ggaaaatatc cggccgtcgc gcagggtgat aagccgccgc gcatggtcca tgatcatctg
      181 gtcgtgggtc gagaagagga aagtgacgcc gtgcgtccgg ttgagttcct gcatcatgtc
      241 gagcagggcg tcggccgtct tcgagtcgag gttcgcggtc ggttcgtcgg cgagtatgat
      301 gtcggggcgc gagacgatgg cgcgcgccac cgccacccgc tgctgttggc cgcccgacag
      361 gtgtatcggc aggcgtgtct gcatcccgtc gagccccacc tcggtcagta tcttcgccac
      421 ccgctcgcgc cgctcggctt tcggtacccc ttgcagaagc atcacatatt cgatgttctc
      481 ctcgacggtg aggaccggga tgaggttgta actctggaaa atgaatccga tatgatcgcg
      541 ccggaagtcg gagagatccg acgcgctcat cgcggtcatc gccttgccgc ccagctccac
      601 cgtgccgcca tcgggcttgt cgaggccgct gacgatattg agaagggtcg atttgccgct
      661 tcccgatggc cccgcgatgg cgctgaattc ccctttcgcg atggtgagcg atacctcttt
      721 caatgcctcg accgtcacct cgccggtgcg gtatatcttt gaaacccggt cgatcctgat
      781 gacatgttcc atcgtatgct ccctcatgtt atagaccgcg tttcatcgct ttggcggggg
      841 tgagccgcgc cgcgtatatg gccggataga gtccgacaag gcaggtgaat atgaacagga
      901 gtaggggata gagggtgaac tgcgtcacgg tgaggaccgg atagatcgga tcgcgcaggg
      961 tcaccacgaa ctcgctgccg ctgtagtcta tgccgtagtg catcagcagg ccgttgacga
     1021 taaatgcgat gacgatcccc atcgcgatgg cgatcagggc gagcgccccc gcctccatga
     1081 ccatcagttg cgccgcctgt cgcggtcgcg tcccgacggc ccgcatcacg ccgaattcga
     1141 acatccgttc atagagcgac atgaaaaggg tgttcacgat gccgaccgcg acgatgccga
     1201 acaggatgat cccgacgagg tagaggctga aatcggtcat cgagcgcatc atggcgagtt
     1261 ccgacaccag ttcggtccac gaaagggcct cgttgccgcc cgccgaaagc tcgctccaga
     1321 gcggcagggc cgtatcctcg cttttcagat tgtcgtggaa cgaaaaggcg atctcgtgca
     1381 gaccgccgtc cagtttcagc atctcctgcg accgtttcag cgggatgaag gccattccgc
     1441 cgtccatctg gcgcgacccg aagcggaaga tgccgccgac gcggaacagt tcctgcgaca
     1501 gttcgccggt gtgggcctgc gccaccgtca cgacgatctt gtcgccgagc cccacctcca
     1561 gcaggtcggc cgtcttttcc cccagcagta tcttcgcttc gtcggctgtg gcgaggtatg
     1621 cgcccgatac gagcaggtcg tctatcttcg aaagttcctt ctcgtcggcc ggatcgatgc
     1681 cgtagagcat caccgactgg acattggcgg ggctcgtgat catcccgaag gaggcggtgc
     1741 gcggagatgc ccggcgtatg agcggctctt ttttcagttt gtcgaagagc gccggaccgt
     1801 tcactatggt ctgttccacc tcgaaggtct tgcggaagcc gtcgcggtgt atctgcccct
     1861 cgccgaggaa cgacgaggtg gcggtcgcga tcatgttgtc gatggtcccc ttcatgacgg
     1921 cgtcggtcgt gatcatcgcg gcgagcccga gcccgatcat gagccccgcg agtatcgtcc
     1981 gccgtttgtt gcggaagagg ttgcgccacg cgagttttac gaagagcatc atggcggcct
     2041 cctagagcga ccgcagcgcg tcgatcggac ggatccgggc ggcgcggatg gcggggagga
     2101 cagagaccag cacggcggtc aacaccacga tgaggagcgg tttatagtag gcgatggccg
     2161 agaactcgct gcggagcgtc gtgagggtga gtccggcgat ggtcaccggt tcggggtaac
     2221 tgatgccgac cgcgacgaaa taccacagcg cgaacgaggc gaggaaggcc ccgatgagac
     2281 acgaggcgag cgcgagcatc gccgtttcga tgacgatgag cgagaatatc cgtcgggggc
     2341 tcgtgccgat ggccgccatc acgccgtatt cgcgggtccg ctctaagacc gtcatgagca
     2401 cggtgttgag cacccccacc gcgacgatga gcatgatgat gaactggctg atgttgtctc
     2461 ctttccggtc ggcctgcatg gtgcggtaga actccggctc gacctccatc cacgattcgg
     2521 ccgacaggtc gcggaatccc gccgtcgcaa gcgtcgcgcc gagccgttcg gtcatcttct
     2581 ccgctttttt gtggtcggcg aagaggaccg cgatctcgtg tatcttcccg cccagcatca
     2641 gatagtcctg cgcgtcggtg atatggagat agacattatt ctgttcggag gtgtccttcg
     2701 cgtcgccgag aatgccgccg acggtgaaca ggtcgttggc gaccgatccg tcggccccct
     2761 gcgagatgag gaccagttcg ctccccacct tcgccttgag gacatcggcg acattacggc
     2821 cgatgagcgc ctgtttcgcg gcggtcgccg gaaggaaggc cccctcgctc accttggcgg
     2881 cgaggcgggt ggtcctcttt tcgcggtcgg gatcgatgcc gatcagtttg acgccggtgg
     2941 tcttcgtctc gacaaaggcg agcgcggagg aatggatgcg gggggtcgcg gcgcggacct
     3001 gagggtcgcc ggttattgcc gcgtagagtt ccggtgtgtc ggcgaagttc ttgtagagcg
     3061 acggccggtc g
//
LOCUS       DAOQUE010000355         3061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_37233_length_3061_cov_4.007651, whole genome shotgun sequence.
ACCESSION   DAOQUE010000355 DAOQUE010000000
VERSION     DAOQUE010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37233_length_3061_cov_4.007651"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1021
                     /locus_tag="PKH10_10240"
     CDS             179..1021
                     /locus_tag="PKH10_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28543.1"
                     /translation="MKRFALLLLVTVGVCLPLQGRGKMDFMLYNLGNTPEGIADFEKM
                     ATQFSAVIAPMFHGEAESRGADGFELGVGYTFTNINLTSRYWTNALSDKPTDMQVPSF
                     YNGIDIHMKKGFSFGLVFYGNLRYYILSEMLSGGVGMEYVLNEGLKSWPDVSIGAGYN
                     GLFGGYDLNMHQAELRVKISKTFVAKKELKLMPFFAYSHLFSWAWSDRLGGYWPMPDD
                     PISYYGDESGNAFYFDQEFINIDRLMIGFKLSHGNFGFSIEAGLPFNAYKAFNLNSGI
                     AVVF"
     gene            1033..1704
                     /locus_tag="PKH10_10245"
     CDS             1033..1704
                     /locus_tag="PKH10_10245"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28544.1"
                     /translation="MIDIHSHLLHQLDDGPESVEESRECLRILAEFGFDEIIPTPHRF
                     HMLYSATPEDTADRMAEIGSLLVRRFSFEYMYGNDLLESSGDLFELATTPSGWKVLLV
                     EFLPLMSRKSDIEQAVFSLNLKGIAPLIAHIERYTMPDEVWKELKKRYSLYYQISLKT
                     LSYGFFDNKRTQILRLLDAGLIDNAATDLHRLAKLQQIEQGLDLLRRKYPEQVERLFS
                     LSFED"
     gene            complement(1705..>3061)
                     /locus_tag="PKH10_10250"
     CDS             complement(1705..>3061)
                     /locus_tag="PKH10_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNT28545.1"
                     /translation="AVTGTPVWSTGDTVWTLDPDSDLATDTTYTVTLKNTIADTIGNT
                     MNEEVFAFTTIDTIAPTVLSTDPADGTDPASITLAAITVLFSEQVINVDETTMTVERT
                     DDGTPVVGTVSLSTDGLTATFAPAAPLEDTTEYTVTLNPDGVTPAVSDPADNPLVGND
                     GGRYLFSFTTGASVCNDGYKTGPEACDDGNNDEGDYCAGNCSATTDPGLVAVVSSVTY
                     DSTPVVRYCPNSTTYLGTGNRNSGRYCGDAQTDYTISLGRNRAYMNFYSDSEATGTGF
                     ELWSGTPGASTLLVKYPASGSYPNSDGKSWRLNAIDLLNNMTLRILNVEENGGTNCYD
                     YVKFFYCDNFTHTDQGITSGCDLRGLYTGTGTPPALANYPTNRMFVRLTSDASTQDDG
                     WQLTANGTAYQCSPYNNYSANMNQVVPIITDTAPIALQFTTLRTESTYDFVYLYTCPP
                     Q"
BASE COUNT          607 a          909 c          886 g          659 t
ORIGIN      
        1 cgagccacgc cgtcgcaaag agaagcagga tagcgccgtt cataacgcac ctccgccgcc
       61 gttcgctctc gatatactcc gatttctcga ccggtcaaca cgactcgcgg actttcttga
      121 catcgccccc tcgttttgtt agggtgccct gtaccggttg catactaccg agggtgacat
      181 gaaacggttc gcgctccttc tgctggtgac ggtcggcgtt tgtctgccgt tacagggccg
      241 aggaaaaatg gacttcatgc tgtataatct cggcaacacc cccgagggca tcgccgactt
      301 tgaaaagatg gccacccagt tctccgccgt catcgcgccg atgttccacg gcgaagccga
      361 gtcgcgcggc gctgacggtt tcgaactcgg cgtcggctac accttcacga acattaacct
      421 cacctctcgc tactggacga acgccctcag cgacaaaccc accgacatgc aggtgccgag
      481 tttctacaat ggcatcgaca tccatatgaa gaaagggttc tccttcggtc tcgtgttcta
      541 tggcaacctc cgctattaca tcctctcgga gatgctgtcg ggcggcgtcg ggatggaata
      601 tgtgctcaac gagggcctca agagctggcc cgatgtctcc atcggcgccg ggtacaacgg
      661 gctgttcggc gggtacgatc tcaacatgca tcaggccgag ttgcgagtta agatatcgaa
      721 gaccttcgtg gcgaaaaaag agctgaaact catgcccttc ttcgcctaca gtcacctctt
      781 ctcatgggcg tggtccgacc ggctgggcgg gtactggccc atgcccgacg atccgatctc
      841 ctactacggt gacgaaagcg gcaatgcctt ctatttcgac caggaattca taaacatcga
      901 ccgcctgatg atcgggttca aactttccca cgggaacttc ggtttcagca tcgaggcggg
      961 actcccgttc aatgcataca aggcattcaa cttgaattca gggatcgcgg tggtcttcta
     1021 gagcgccgcc ccatgataga catccactcc catcttctcc accaattgga cgacggcccc
     1081 gagtcggtcg aagaatcacg ggaatgcctt cgcatcctcg ccgaattcgg ctttgacgag
     1141 atcatcccca ccccgcaccg tttccatatg ctctacagcg cgacccccga agataccgcc
     1201 gaccgcatgg ccgagatcgg ctcgctcctc gtccgccggt tctcgttcga atatatgtac
     1261 gggaacgacc tgttggaatc gtcgggagat ctgttcgaac tggccaccac cccatcgggc
     1321 tggaaggtgc tcctcgtcga attcctgccg ctcatgtcga gaaagagcga catcgaacag
     1381 gcggtcttct cgctcaacct caagggcata gccccgctta tcgcccacat cgagagatat
     1441 accatgcccg acgaggtctg gaaagaactc aaaaaaagat actcgctcta ctatcagatc
     1501 agcctcaaaa cgctttctta cggttttttc gacaacaagc gcacccagat cctccggctc
     1561 ctcgacgctg ggctcatcga caacgcggcg accgacctgc accggctcgc aaagttacag
     1621 cagatagagc agggactcga tctcctgcgc cggaagtacc cggaacaggt cgagcggctc
     1681 ttctcgctct ctttcgagga ttagttattg tggcggacag gtgtagagat acacgaaatc
     1741 gtaggtcgat tcggtccgca gcgtcgtgaa ctgcaacgct atcggcgccg tatcggtaat
     1801 tatcggcacg acctgattca tgtttgcgga ataattgttg tagggtgaac attgataggc
     1861 ggtcccgttc gccgtcagtt gccaaccgtc atcttgcgta ctggcgtcgc tcgtcaaccg
     1921 cacgaacatt cggttggtcg gatagttcgc gagggcgggc ggcgttccgg tgccggtata
     1981 gagcccgcgt agatcgcagc cgctcgtgat gccctgatcg gtgtgggtga agttgtcgca
     2041 gtagaagaac ttcacataat catagcagtt ggtcccgcca ttctcctcca cattcagtat
     2101 ccgcagggtc atgttattca ggagatcgat cgcgttgagc cgccacgact tgccgtccga
     2161 gttcggatat gatccggacg cgggatattt cacgagcagt gtcgatgcgc cgggggtgcc
     2221 gctccacagt tcgaagccgg tccccgtggc ctccgaatcc gagtagaagt tcatgtaggc
     2281 gcggttccga ccaaggctga tggtatagtc ggtctgagca tcgccgcaat acctgccgga
     2341 gttccgattc cccgtcccga ggtaggtggt gctgttcgga cagtaacgga cgaccggcgt
     2401 gctgtcataa gtaacggagc tcaccaccgc gaccagcccc gggtcggtcg tcgccgagca
     2461 gttgccggca cagtagtctc cctcgtcatt attgccgtcg tcacacgcct ccggtccggt
     2521 cttgtagccg tcgttgcaga cgctcgcacc ggtggtgaag gagaagagat acctgccacc
     2581 gtcgttgccc acgagcgggt tgtccgccgg atctgaaacg gccggggtga cgccgtcggg
     2641 gttgagcgtg acggtgtact cggtggtgtc ctcgagcggc gcggcggggg cgaaggtggc
     2701 ggtaagcccg tcggtcgaga gcgagaccgt accgacgacc ggagtgccgt catcggttct
     2761 ttcgacggtc atcgtggtct cgtcgacatt gatcacctgc tcactgaaga ggacggttat
     2821 cgccgcaagg gtgatcgagg cggggtcggt cccgtcggcc gggtcggtcg acaggacggt
     2881 cggcgcgatg gtgtcgatcg tggtaaaggc aaagacctcc tcgttcatgg tattgccgat
     2941 ggtatcggcg atcgtatttt tcagcgtgac ggtgtaggtg gtatcggtgg cgagatcgct
     3001 gtccggatca agcgtccaga cggtgtcgcc cgtcgaccag accggcgtgc cggtcaccgc
     3061 g
//
LOCUS       DAOQUE010000356         3055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_37347_length_3055_cov_2.985667, whole genome shotgun sequence.
ACCESSION   DAOQUE010000356 DAOQUE010000000
VERSION     DAOQUE010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37347_length_3055_cov_2.985667"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2610
                     /locus_tag="PKH10_10255"
     CDS             <1..2610
                     /locus_tag="PKH10_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT28546.1"
                     /translation="ELLMSDPRLALFSFLCGVLLFLGNTVGNALFIQYYRPETLVFIY
                     LATGILTALVSLGALLRRYRMTIVLPAFLAVMVAVMAFFSFLYALMPAYRREAVAMLF
                     FATTVGYMLYGQFFVQFLSARFDVRQMKERYGVIQTGEVLAYLAVGLAVPLALSLTAL
                     PVAAILMPALLCGVGAIVVFFSFRSVAAPARPAPDRRAVSKDRSAFRQHRRHAYLWMI
                     ITFTVCQAMAYCIVDYLYNRGMKQQFSSPEQLAFYFALFSAIAKLTASALSITVSRSV
                     LQRIGVGGGLALLPMVVTAMLALAGIASLSGLPAATLIFACFVAGKMSFSMVGIPFAN
                     PALSTLLQLFPAARHERMVAVVLGMVRPLASALGGLVLYFAFYVVGSGRPMTLMSGIA
                     LLLAVWLIMAFALYSRYAQRLRVLLGTLLQPHGAVVVDRETLDDLEARMLADRNYLAT
                     VQTLALLEEHRLERLENIVRKKFLHANGEEEKHLLSCIERHGWPAFTEQVRTLCLQRK
                     RPDVAAAAIQCLGAIGDDGDIETVARGLDPANGELHDRLLSISLRRGVVSERARAELD
                     RSAFSREERERRRLLSVYRHAGVPLPAAYRERLLEGASPPVLREFIKYAGAMKDLDGY
                     PHIVNGLSDPMFCMTAHKAAGAVGEPIAPALVERYREETDPYARRMLLMTMARIGGEA
                     VWRFVHERMTASSPDEARLLAVVFLGKRLPSGLFTDGELGRLREMAITRTDLLWGLRD
                     RVSPGHKGRRWHEAVDEELSYEAALLLFSMSTERSVSDLPLIRRHLFSDDRKQAAVAV
                     ELLEKELGHDRFVLLAPVLDRIIRRQEGDADGGDPLSQDEAKKVLSEQKGYSAYFVAM
                     MEQLTGEDSDATT"
     gene            2597..3052
                     /locus_tag="PKH10_10260"
     CDS             2597..3052
                     /locus_tag="PKH10_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT28547.1"
                     /translation="MRQLEKVYLLKQTPLFMQVKGDHLMALARFMEERYTQPQEVIVQ
                     KGDDGETLYLVAAGRFQVSNKEQVLAQLGTGSLFGELAALVPEKRTATVSSLDEGLLL
                     SLSHDAVQSLILLQPEIALDIIEYLVRRIRENPYQMYTKTILAQLPTRP"
BASE COUNT          498 a          912 c          975 g          670 t
ORIGIN      
        1 gaacttctga tgagcgaccc gcgcctcgcg ctcttttcct ttctctgcgg cgtcctccta
       61 ttcctcggca acactgtcgg gaacgccctg ttcatccaat actaccggcc cgagacgctg
      121 gtgttcatct atctcgccac cggcatcctg accgcgctgg tcagtctcgg tgcgctcctg
      181 cgtcgttacc ggatgaccat cgtgcttccc gcgttccttg ccgtgatggt cgcggtcatg
      241 gcgtttttct ctttcctgta cgccctgatg cccgcctacc gccgtgaagc ggtggccatg
      301 ctcttctttg ccacgacggt cggctacatg ctttacggcc agttcttcgt gcagttcctg
      361 agcgcccgtt tcgatgtccg ccagatgaag gagcgttacg gtgtcatcca gacgggcgag
      421 gtgctcgcct atctggcggt cgggctcgcc gtgccgttgg cgctctcact gacggcgctc
      481 cccgtcgcgg cgatcctgat gccggcactg ctttgcggcg tgggagcgat cgtggtcttc
      541 ttctcgttcc ggtcggtcgc ggcgcccgcg aggcccgcgc ctgatcgacg tgccgtatcg
      601 aaggatcgga gcgcattccg tcagcaccgg cgccacgcgt atctttggat gatcatcacg
      661 ttcaccgtgt gtcaggcgat ggcctactgc atcgtggact atctgtataa ccgcggcatg
      721 aagcagcagt tctcgtctcc cgaacagttg gccttttact tcgcgctttt ttcggcgata
      781 gccaaactga ccgcctccgc cctctctata accgtctccc gttcggtgtt gcaacgcatc
      841 ggcgtcggcg gcgggctggc gcttctgccg atggtcgtga ccgcgatgct ggcgctcgcc
      901 gggatcgcgt cgctttcggg gcttccggcc gcaacgctca tcttcgcctg tttcgtggcc
      961 ggcaagatga gtttcagcat ggtgggcatc ccgttcgcca accccgccct ttcgacctta
     1021 ctccagttat tccccgcagc tcgccacgaa cggatggtgg cggtggtgct cggcatggtc
     1081 cgtcctctgg cgagcgcgct cggagggctg gtgctgtact tcgcgttcta tgtcgtggga
     1141 tcgggtcggc ccatgacgct gatgagcggc atcgcgctgc ttctggccgt ttggcttatc
     1201 atggccttcg ccctctattc gcggtatgcc cagcggttga gggtgttgtt gggaacgctt
     1261 cttcagccac acggcgcggt ggtggtggat cgggagacac tggatgatct ggaggcccgg
     1321 atgcttgccg accggaacta tcttgccacc gttcagacgc ttgctcttct ggaggaacat
     1381 cgcctcgagc gactggagaa tatcgtgaga aagaaattcc tccacgcgaa cggagaagag
     1441 gagaagcatc tcctttcctg catagaacgg catggatggc ctgcttttac ggagcaggtg
     1501 cggacgctgt gtctgcaacg gaaacggccg gatgtcgccg ccgccgccat ccaatgtctg
     1561 ggcgccatcg gtgatgacgg ggatatcgag acggtggcga gggggctcga tcccgcgaac
     1621 ggcgaactgc atgaccgtct cctttcgatc tcgctgcggc gcggtgtagt atctgagagg
     1681 gcgcgggccg agcttgaccg atcggcattc tcccgtgagg agcgtgagcg ccggcgcctt
     1741 ctttcggtgt atcgtcacgc gggtgtgccg cttcccgcgg cctaccggga acgcctgctg
     1801 gaaggtgcat cgcccccggt gcttcgtgaa ttcatcaagt atgccggcgc gatgaaggac
     1861 cttgacgggt atccgcacat cgtcaacggg ttgagcgatc cgatgttctg catgacggct
     1921 cacaaggccg ccggggccgt gggcgagccg atcgcccccg cgctcgtcga gcggtatcgc
     1981 gaggagaccg acccgtacgc ccggcgcatg ctcctgatga cgatggctcg gatcgggggg
     2041 gaagcggtgt ggcgtttcgt gcatgaacgg atgacggcat cgtcgcccga cgaggcgcgg
     2101 cttctggccg tggtgttcct tggtaaacgg cttccctccg gtctcttcac cgacggggaa
     2161 ctcggtcgct tgcgtgagat ggcgatcacg cgaacggatc tgctctgggg cctgcgcgac
     2221 agggtgtcac cgggtcataa aggacggcgg tggcatgagg cggtcgacga ggaattgagt
     2281 tacgaggcgg cgctgttgct cttttcgatg tccacggaga gatcggtttc cgacctgccg
     2341 ctcatccggc ggcatctttt ctccgatgac cgcaagcagg cggcggtcgc ggtcgaactg
     2401 ctggaaaagg aattgggaca cgaccgtttc gtgctgctgg ctccggtcct tgaccgtatc
     2461 atccgtcggc aagagggcga tgcggacggg ggcgatccct tgtcgcaaga cgaggcgaag
     2521 aaggtcttga gcgaacaaaa aggatattcg gcctatttcg tggcgatgat ggaacagttg
     2581 acgggagagg atagcgatgc gacaacttga aaaggtgtac ctgctcaaac aaaccccgct
     2641 tttcatgcag gtcaagggcg accacctcat ggcgctcgct cgtttcatgg aggagcgcta
     2701 cactcagccg caggaggtca tcgtgcagaa aggagatgac ggcgaaacgc tgtatctggt
     2761 cgccgccggc cgctttcagg tgagcaataa ggaacaggta ctcgcgcagt tgggcaccgg
     2821 gtcgctgttc ggcgaactcg ccgcgctggt gcccgaaaag aggaccgcca cggtttcctc
     2881 gctcgatgag ggactgctcc tgtcgttgtc gcatgatgcg gtgcagtcgc tgatactgct
     2941 tcaacccgag atagcgttgg acatcatcga atatctggtg cggcgcatcc gcgagaaccc
     3001 gtatcagatg tacacaaaga ccatactcgc tcaactgccg acgaggccgt agttc
//
LOCUS       DAOQUE010000357         3053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_37381_length_3053_cov_4.166444, whole genome shotgun sequence.
ACCESSION   DAOQUE010000357 DAOQUE010000000
VERSION     DAOQUE010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37381_length_3053_cov_4.166444"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PKH10_10265"
     CDS             <1..416
                     /locus_tag="PKH10_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28548.1"
                     /translation="PSETPAPVAVAPVTTAPEAMPQAPAMVEEEPTPSRELTALGWSG
                     VGLLAGGGAVMVLAGIAHWQTIEAKDDYDWGRTDSRSTYDGWRGTMIASYAVGGAALG
                     VGTALLVWDLVAERPVRTAVVPMPGGAMVSVAWRW"
     gene            418..>3053
                     /locus_tag="PKH10_10270"
     CDS             418..>3053
                     /locus_tag="PKH10_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT28549.1"
                     /translation="MRKVLLFVWAVFLIVSCDAIKLTERGGEGEACYLDNTCKEPFEC
                     VDGTCVKKTADTDDIAETDDELVVGDEDEDGEKDEKDISDAKDGDEGEGEDDAVGDDV
                     ADDDTTDDDTTDNLSDVTDASDEIVTDDAVIVADEDSDDDQPDPTDSSDEPVGDDTVV
                     VTDDDVVVTANVLCTGQTKCYDATAEMTCPTEGNDFYGQDAQYAVLGFCTPRDYTVSG
                     TSGNDVVTDNVTGLIWQRTLPATYAGCTGESGTKCTWQEAVDYCANLSYGGSDDWRLP
                     TRKELATLADYGKNNPAIDTAMFPGTQSNYYWSSSPYVNNTSFAWVVYFIYSTIGADD
                     KMNPNYARCVRGATLPDSVFTEATVAGKVMVTDTTSALIWTKEYSASTLTWKSALDYC
                     ETLDYGDATDWRLPNIEELKTLIDDTRYNPASSFPGGIPTNWFWSSSSYMSSTTYAWS
                     VNSGSGNVSSNNKTDNYYARCVRSEPIAPVDECITQSNPCDEGGDANAICDDEAVGYT
                     CNCSLNYTDDGTTCAPDTRTFYCAAKPVNADWNTVDSYEQTWDGAAWVPDDSVTAYNA
                     TASTTACRFKCAAGYYWNGDSCINERIVPCNGIIANAIYYGGETTYSITQTYTIADGW
                     GPDTDAFYADGIADEGTCEYQCNTNYSWTGEECLADMRTFTCDAKPAHTDWNTVSSYQ
                     QTWIDGNTWDPADDPDTDYNPTGDTASCRYKCVAGYYWNGSYCVNDKVTCTGQSKCYD
                     NSAEMTCPPWNSAFFGQDYQYSWGQCNARSYTVSGTAPNDVVVDNNTGLIWQRTLPAT
                     YAGCTGGAPTGSTCMWQEAVDYCENLTYGGQDDWRLPSRKELATLPDYGYSNPAIDTT
                     TFPDTQSGFYWASTPYVNDAAQS"
BASE COUNT          683 a          859 c          955 g          556 t
ORIGIN      
        1 cgccatccga aacgccggcg ccggtcgctg tagcgccggt cactacagcg ccggaagcca
       61 tgccgcaggc ccccgcgatg gtcgaggagg agccgacccc ctcgcgcgaa ctcaccgcgc
      121 tcggctggag cggcgtcggt ctccttgcgg gcggcggtgc cgtcatggtg ctggcgggta
      181 tcgcccactg gcagacgata gaagcgaaag atgattacga ctggggaagg accgatagcc
      241 ggagcaccta cgacgggtgg cgcggcacga tgatcgcctc gtatgcggtc ggcggtgcgg
      301 cgctgggcgt ggggacggcg ctcctcgtct gggatctggt ggccgaacgg ccggtgcgga
      361 cggcggtggt gccgatgccg ggcggggcga tggtatccgt ggcgtggagg tggtgatatg
      421 agaaaggtgc tgttattcgt gtgggccgtg ttcctgatcg tttcctgcga cgcgataaaa
      481 ctcaccgagc gcggcgggga aggggaggcc tgctatctgg acaacacctg caaggagccg
      541 ttcgagtgcg tggatgggac ctgcgtgaag aagacggccg ataccgacga tatcgccgag
      601 accgacgatg agttggtcgt aggggacgag gatgaggacg gggaaaagga cgaaaaggac
      661 ataagtgacg caaaggacgg ggatgaagga gaaggggagg atgacgccgt cggcgacgat
      721 gtggccgatg atgataccac cgatgatgat accaccgata atctatccga tgtcaccgac
      781 gcgtccgacg aaatcgtgac cgacgatgcg gtcatcgtcg ccgacgagga ttccgacgac
      841 gatcaacccg atccgaccga ttcgtcagat gagccggtgg gcgacgatac ggtcgtggtc
      901 actgatgatg atgtggtcgt gaccgcgaat gtcctctgca cggggcagac gaagtgctac
      961 gatgcgacgg cggagatgac ctgtccgacg gaaggcaacg atttctacgg gcaggatgcg
     1021 cagtatgcgg tgctggggtt ctgtacgccg cgcgactaca cggtgagcgg cacaagcggc
     1081 aatgatgtcg tcaccgataa tgtgacggga cttatttggc agagaacttt gccggcgacc
     1141 tacgcggggt gtaccggcga gagcggcacg aaatgcacct ggcaagaggc ggtggattac
     1201 tgcgccaacc tttcgtatgg cggttcggat gactggcgct tgccgacgcg caaggaactg
     1261 gcaacgctag ctgactatgg caaaaacaat cctgcaatag ataccgcaat gttccccggc
     1321 acgcaatcca actattattg gtcttcctcg ccttatgtaa acaacacctc ttttgcatgg
     1381 gttgtgtatt tcatctatag caccattggc gctgatgata agatgaaccc gaattatgcg
     1441 cgttgtgtga gaggcgcgac attgcctgat agcgttttca ctgaagcaac ggttgcgggc
     1501 aaggtgatgg ttacggatac gacatccgct cttatctgga cgaaggaata cagcgcaagc
     1561 actttgacat ggaagagtgc cctcgactac tgcgaaacat tggattacgg cgatgccact
     1621 gattggcgtc tgcccaacat agaggaatta aaaacgctta tagacgacac acgttacaat
     1681 ccagccagta gtttcccggg aggaatacct accaattggt tttggtcctc ctcgtcctat
     1741 atgagcagta cgacctatgc atggagcgta aattctggca gtggcaatgt gagtagtaat
     1801 aataaaactg acaactatta tgctcgttgc gttcgaagcg aaccgatcgc tccggttgat
     1861 gaatgcatca cgcagtctaa tccgtgcgac gaaggcggcg acgccaatgc gatctgcgac
     1921 gacgaggcgg tcggctacac ctgcaactgc tcgctcaact acaccgacga cggcaccacc
     1981 tgcgcccccg atacccgcac attctactgt gccgcgaaac cggtcaacgc cgactggaac
     2041 accgtggaca gttacgagca gacatgggac ggcgccgcgt gggtgcccga cgacagcgtg
     2101 acggcgtata atgcgaccgc cagcaccacc gcctgccgct tcaagtgcgc ggcggggtac
     2161 tactggaacg gcgactcgtg catcaacgaa cggatcgtcc cctgcaacgg catcatcgcc
     2221 aacgcgatct actacggcgg tgagaccacc tattccatca cgcagaccta caccatcgcc
     2281 gatggctggg ggcccgacac cgacgccttc tacgccgacg gcatcgccga cgaggggacc
     2341 tgtgagtacc agtgcaatac caactactcg tggaccgggg aagagtgcct cgctgatatg
     2401 cggaccttca cctgtgatgc caaaccggcg cataccgact ggaacaccgt ttcttcctac
     2461 cagcagacat ggatcgacgg caatacatgg gatcccgccg acgatccaga caccgactac
     2521 aacccgaccg gtgataccgc ctcctgtcgc tacaagtgcg tggcggggta ctactggaac
     2581 ggttcctact gcgtgaacga caaggtgacc tgcaccgggc agtcgaaatg ctacgacaac
     2641 agcgccgaga tgacctgtcc gccgtggaac agcgccttct tcggacagga ctaccagtat
     2701 tcgtggggtc agtgcaacgc gcgcagttac acggtgagcg gcactgcacc gaacgatgtg
     2761 gtcgtggaca acaatacggg gctgatatgg cagaggacgc ttccggcgac ctacgccggg
     2821 tgcaccggcg gcgcgccgac cggttcgacc tgcatgtggc aggaggcggt ggattactgc
     2881 gagaacctca cctacggcgg gcaagacgat tggcgtctcc cgtcccgtaa ggaactggcg
     2941 accctgcccg actatgggta ttcgaacccg gcgatagaca cgacgacatt ccctgataca
     3001 cagtcgggtt tctattgggc ctccaccccc tatgtgaacg atgccgccca atc
//
LOCUS       DAOQUE010000358         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_38091_length_3014_cov_4.410950, whole genome shotgun sequence.
ACCESSION   DAOQUE010000358 DAOQUE010000000
VERSION     DAOQUE010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38091_length_3014_cov_4.410950"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="PKH10_10275"
     CDS             complement(<1..1252)
                     /locus_tag="PKH10_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNT28550.1"
                     /translation="MTTQYVYFFGPDGSEGNATMKNALGGKGANLAEMSNLGIPVPAG
                     FTISTDMCTVYYKMDRKYPPELKKQVAENLAKIEKVMGKKFGSTRNPLLVSVRSGARA
                     SMPGMMDTILNLGLNEETLKGLIRKPGDERFAWDSYRRFIQMYGDVVMGLKPENKDEE
                     DPFEVVMHELKKERKAKQDLDLTTADLKELVKRYKAMIKRKLKKDFPTDPEEQLWGAI
                     NAVFGSWMNDRAIRYRKMNEIPDEWGTAVNVQAMVFGNMGADCATGVAFTRNPATGEN
                     KFFGEYLINAQGEDVVAGIRTPQQVTLEGSRKWAVENGIPEKVRKTQYPSLEEYMPDT
                     YKQLVAIYTKLEKHYRDMQDIEFTIEGRKLYMLQTRNGKRTAAAAVKIAVDMVKEGLI
                     DRDEALLRVKPADLDQLLHPQFIKA"
     gene            complement(1342..2649)
                     /locus_tag="PKH10_10280"
     CDS             complement(1342..2649)
                     /locus_tag="PKH10_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNT28551.1"
                     /translation="MRKMHALLTLIFASLRGRLFATTVSILAVAVALAVVAVTGIVSF
                     AIAKQAFESGRKYPLIVAAEGSSETQIILSTIFHIDKPRGKIPFETYETLKKDPRVTA
                     VFPIAAADSVGVFPIIGTDGAFVADLGVGYRGDIALADPHDAVLGATVARQLLMDVGT
                     EFTGTHGMGGSADEAHEHADHTYIVKGILDPTGGPEDAAVYTSYKAVWEIHGEKGHKG
                     HEGHKGQGEEKEDHDAHHDHVPSVPESDHDHDRYHLSDGMLTAVLARTGNPVQVFDLQ
                     QEYSKPGLTAVNTAGAVRSFQKYFNTAGLVATFFAGLTLLIAAALILAITLMALDARR
                     RELALLRILGIGRGMIAVLIMLETLIVTVTGVVIGFLLGHGIPSLFAADISRWVGASV
                     EPWHIGANELPAIIGALLLGQAVALIAMLRAYRMDLVEESGKN"
     gene            complement(2642..>3014)
                     /locus_tag="PKH10_10285"
     CDS             complement(2642..>3014)
                     /locus_tag="PKH10_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT28552.1"
                     /translation="TIIDNAVLPHILIGSGAKGDLEKRAEASLATLGIGDHLRKYPHQ
                     LSVGQRQRVAVARALLASPRLIIADEPTANLDDASARIVADAILALRGRSTIFIASHD
                     GRFGAVKPDLTIALGGEAGHA"
BASE COUNT          554 a          903 c          931 g          626 t
ORIGIN      
        1 cggccttgat gaactgcggg tggagcagtt ggtcgaggtc ggccggtttc acgcgcagga
       61 gcgcctcgtc gcggtcgatg agtccttcct tcaccatgtc gacggcgatc ttgaccgccg
      121 ccgccgcggt ccgcttgccg ttacgggtct ggagcatata gagtttgcgc ccctctatcg
      181 tgaactcgat gtcctgcatg tcgcggtagt gcttctcgag tttcgtatag atcgcgacga
      241 gttgcttgta ggtgtcgggc atatactcct cgagcgaggg atactgcgtt ttgcggacct
      301 tttcgggtat gccgttctcg acggcccact tccgggaacc ttcgagcgtg acctgttgcg
      361 gggtacggat accggcgacg acatcctctc cctgtgcgtt tatgaggtat tcgccgaaga
      421 atttgttctc gccggtcgcc ggattgcgtg tgaaggcgac accggtggcg cagtcggcgc
      481 ccatattgcc gaacaccatc gcctgcacat tgaccgcggt cccccactcg tcggggatct
      541 cattcatctt gcgataccgg atggcgcggt cattcatcca cgagccgaac accgcgttga
      601 tggcgcccca cagttgctcc tcggggtcgg tcgggaaatc ctttttgagt ttccgcttta
      661 tcatcgcctt gtagcgcttg accagttcct tgagatcggc ggtggtcaga tcaagatcct
      721 gtttagcctt gcgctctttc ttgagttcgt gcatcacgac ctcgaagggg tcctcctcgt
      781 ccttgttctc cggcttgaga cccatgacga catcgccgta catctggatg aaacggcggt
      841 aggaatccca cgcgaaccgc tcgtcgcccg gtttacggat gagccccttg agcgtctcct
      901 cgttgagccc gagattgagg atggtgtcca tcatgccggg catcgaggcg cgggcgcccg
      961 agcggaccga cacgagaagc gggttgcggg tcgaaccgaa cttcttcccc atcacctttt
     1021 ctatcttcgc cagattctcc gcgacctgtt tcttgagttc gggcggatat ttccggtcca
     1081 tcttatagta gacggtgcac atatcggtcg agatggtgaa gccggccggt accggtatac
     1141 ccaagttcga catctcggcg aggttggcgc ctttgccgcc gagggcattc ttcatcgtgg
     1201 cgttcccctc ggaaccgtcg gggccgaaga aataaacata ctgcgtggtc atcgtgtcct
     1261 ccaaaaaagg taagtcaaca cacaacggcg gaatcatata cgcttcacgg agaaattcaa
     1321 tttaattcga tggggcgcgg gtcaattctt cccgctttcc tcgacgaggt ccatgcggta
     1381 cgcgcggagc atcgcgatga gtgcgaccgc ctgcccgagc aggagcgccc cgataatggc
     1441 cggcagttcg ttcgcaccga tgtgccatgg ctcgacggat gcgccgaccc atcgcgagat
     1501 atcggcggcg aacagcgacg ggataccatg ccccagcagg aagccgataa cgacgccggt
     1561 caccgtgacg atgagcgttt cgagcatgat gagcacggcg atcattccgc ggccgatgcc
     1621 gagtatgcgc aaaagcgcca actccctccg ccgcgcatcg agcgccatca gcgttatggc
     1681 aagtatgagc gccgcggcga tgagaagcgt cagccccgca aagaaggtcg cgacgagccc
     1741 cgccgtgttg aagtatttct ggaacgaccg gaccgcgccg gcggtgttga cggcggtaag
     1801 ccccggcttg ctgtactcct gctgcaggtc gaatacctgc accggattgc cggtgcgcgc
     1861 cagcacggcg gtcagcatac catcgctgag atgataacgg tcgtggtcgt ggtcagattc
     1921 agggactgaa gggacatgat cgtgatgggc atcgtgatct tctttctctt ccccctgtcc
     1981 cttatgtcct tcatgtccct tgtgtccctt ttccccatga atttcccaca ccgccttgta
     2041 ggaggtatag accgccgcat cctccgggcc gccggtcgga tccagaattc ccttgacgat
     2101 ataggtgtga tcggcgtgtt cgtgcgcctc gtcggccgaa ccacccatcc cgtgggtgcc
     2161 ggtgaattcg gtcccgacat ccatcagcag ttgccgcgcc accgtcgcgc cgagcaccgc
     2221 atcgtgcggg tcggcaagcg cgatgtcgcc gcggtagccg acaccgaggt cggcgacaaa
     2281 ggcgccgtcg gtcccgatga tcgggaacac gccgaccgaa tcggcggcgg cgatgggaaa
     2341 gacggccgtg acgcgcgggt ccttcttgag cgtttcgtag gtctcgaacg gtatcttgcc
     2401 gcgcggcttg tcgatgtgga agatggtcga aaggatgatc tgcgtctcgc tcgacccttc
     2461 ggcggcaacg atgagcggat acttgcgacc cgactcgaag gcctgcttgg ctatcgcgaa
     2521 cgatacaatg ccggtgacgg cgaccacggc gagcgcgacg gcgacggcga gaatggaaac
     2581 ggtcgtcgcg aagagccgcc cccggagaga tgcgaatatc agcgtcaaaa gagcatgcat
     2641 cttacgcatg gcccgcctcc ccgccaaggg cgatggtgag atcgggcttc accgcgccga
     2701 aacggccatc gtgcgaagcg ataaaaatgg tggagcgccc ccgcaaggca agtatcgcgt
     2761 cggccacgat acgcgccgaa gcgtcatcga ggttggcggt cggctcgtcg gcgatgatga
     2821 gacgcggcga ggcgaggagt gcccgtgcca cggcgacccg ctggcgttga ccgaccgaaa
     2881 gttgatgcgg atatttgcgc agatgatcgc cgataccgag cgtcgccagc gacgcttcgg
     2941 cgcgcttttc aagatcgcct ttcgcaccgc tcccaatgag gatgtggggc agaacggcat
     3001 tgtcgataat ggtg
//
LOCUS       DAOQUE010000359         3012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_38134_length_3012_cov_3.145756, whole genome shotgun sequence.
ACCESSION   DAOQUE010000359 DAOQUE010000000
VERSION     DAOQUE010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38134_length_3012_cov_3.145756"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PKH10_10290"
     CDS             <1..631
                     /locus_tag="PKH10_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNT28553.1"
                     /translation="IFMDMVNNQIPSFEDKAEALYYFPMFRTWFGLCGFCKLPWNDVE
                     PENNREKYPPKEAAKVPEHVDNYLTDFKAVTGRDLDRDGLIRMSARVYNFQRIFNIRR
                     GYGLRANDAQPYRACGPVTKREYESRQERYDKQLVDWMGMAPEAVAKMTAEEKMAAHR
                     KYRTDRYEKLLDAVYKRRGWTKNGVPTIAWLKELGMDLPELLEVITPLQ"
     gene            639..845
                     /gene="thiS"
                     /locus_tag="PKH10_10295"
     CDS             639..845
                     /gene="thiS"
                     /locus_tag="PKH10_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNT28554.1"
                     /translation="MKGKITVNDQVIDWRDGMTVSDVLKVMNYTFKLLVIQIDGQNVK
                     RDDWSTATVPEGADVKVIHLMSGG"
     gene            complement(892..1350)
                     /locus_tag="PKH10_10300"
     CDS             complement(892..1350)
                     /locus_tag="PKH10_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HNT28555.1"
                     /translation="MTTPGRIIVDADACPGPVREILFRTSARRGIVVVLVANRWPEPP
                     KSEFISAVVVPQGPDVADDRIVEMSAPGDIVVTADIPLADRSIAKGALVITPKGHLLT
                     ASNIGERLSIRNAMDEMRGAGIDTGGPAPYSVKDKQRFAAGLDRLLARSS"
     gene            complement(1378..2811)
                     /locus_tag="PKH10_10305"
                     /pseudo
     CDS             complement(1378..2811)
                     /locus_tag="PKH10_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          576 a          875 c          959 g          602 t
ORIGIN      
        1 catcttcatg gatatggtca acaaccagat accgtcgttc gaggacaagg ccgaggcgct
       61 ctactacttc ccgatgttcc gcacatggtt cggcctgtgc ggcttctgca aactgccgtg
      121 gaacgacgtc gagccggaga acaaccgtga aaagtacccg ccgaaagagg cggcaaaggt
      181 ccccgagcat gtcgacaact acctgacgga cttcaaggcg gtcaccggcc gcgacctcga
      241 ccgcgacggc ctgatacgca tgtcggcccg cgtctacaat ttccagcgca tattcaacat
      301 ccgtcgcgga tacggcctgc gcgcgaacga cgcgcagccc tatcgcgcct gcggcccggt
      361 caccaaacgc gaatacgagt cgcgccagga acgctacgat aaacagctcg tcgactggat
      421 ggggatggcc cccgaggcgg tcgcgaagat gaccgccgag gagaagatgg cggcccaccg
      481 taaataccgc accgaccgct acgagaaact gctcgacgcc gtctacaagc ggcgcggctg
      541 gaccaagaac ggtgtcccga ccatcgcgtg gctcaaggag ctcggcatgg acctgcccga
      601 acttctcgag gtcatcacgc cgttacagta agggtctcat gaaaggcaag ataacggtca
      661 acgatcaggt catcgattgg cgcgacggga tgaccgtctc cgacgtgctc aaagtgatga
      721 actatacctt caaactgctc gtcatccaga tagacgggca gaacgtcaag cgggatgact
      781 ggtccaccgc gacggtgccg gagggggccg atgtcaaggt gatccatctg atgagcggcg
      841 gctagccgga aaaagatccg tcaggtctgt gcgctaaacg ggcagggaac ttcaggatga
      901 tcttgcaaga agacggtcaa gccccgcggc gaaacgctgt ttatccttca cggagtaggg
      961 agccgggccg ccggtgtcga tgcccgctcc gcgcatctca tccatcgcat tgcggataga
     1021 gagccgctcg ccgatgttcg acgcggtcaa caggtgtcct ttcggcgtga taacgagtgc
     1081 tcccttcgcg atggagcggt cggcgagcgg gatgtcggcg gtgacgacga tgtcccccgg
     1141 cgcggacatc tcgacgatgc ggtcatcggc gacatcgggt ccctggggaa cgacgacggc
     1201 ggaaataaat tcgcttttgg gcggttcggg ccagcggttc gcgacgagca cgacgacgat
     1261 gccccgccgc gccgaggtgc ggaagagtat ctcgcggacc gggccggggc aggcatctgc
     1321 gtcgacgatg atgcgcccgg gagtggtcat tgaaaaggca tccgaagcac gcgggtatta
     1381 ttccgtcgtg atgccgacga agcgtgtgag cggtccccgg tcggccggtt cggtgaaggt
     1441 gcccgaaact tctttgaata tcgcgccgct gccgcgcagg gtggtgccgg tgcctgtgta
     1501 gaggcgcggc gtgaggccga acccggcccc ttcgaagccg aacggtccgg tctcttcatt
     1561 cagcaacagc gcgatatgcc agttgatgag gaagtcgctc atcgtgagcc ccggcgcgat
     1621 atatttcttt atcgcctctt cgacacagag gtaattgttg ttctggctct ctatgatctc
     1681 gcggaacatc gcattgtccc cgccgccctg cgtgtggagg tattggaaga agaggtaggc
     1741 gagcgcgtag ttgccgtaga ccttgacgct gtcgttgtat tcccagtagg tgagcgattg
     1801 gccatcggct atcgcggcga cctgattgta tgccgctatc cagttgttct gaacgccgtt
     1861 gtagagatgc atggcggtca tggagagtcc ctcgttcatc cagaggttcc ccatgtcgtt
     1921 ggcgccctgc atgtcgcctt cctccagcac attgcggttg ttgttgacaa gatgctggaa
     1981 ctcgtgggtg atggcggcgt aggcttccgg gttgcccggg gaatattggg tagtgacgaa
     2041 tatcatgtcg cgctcgttcg atgagggata ctgctcttgg gaatagagat cgccggggct
     2101 gaagtaaccc gccatgagcg accccagatc agtgaatagg agggtgatgg tcccgtcctc
     2161 gttcacatcg ctcggttcat agaaattctc agtgacgata gggtatatga ccgtatcgaa
     2221 ctcgccggct atcgcggtta tctcctcctc ggtcgcccat tccggctcgc tcgtccatat
     2281 ctggacatgt tcgcctatct tcatgagggt cgcggtgacc tgcgtcatct ggcccgaatc
     2341 gttcgcgacg tagaacgact gggtgtcgcc ctcggtcggc gcgagcggta gtaccggctg
     2401 aaagaggtct gcgttggtca gcccctcggg tatgccgaaa tgcgggttga tgaagaggtc
     2461 gagcccggtg cccgatgtcg ccggcatggc cgagcggagt gccagcatgg tcggcagggt
     2521 accggtcgcc gacatcgtat ccccgttcgg ggagacattg atggccatca cgatatcacc
     2581 ttcgtagtcc ggtccggtga agtcgaaccg gtcaccgtat tcgataaggc aggtgtccga
     2641 acagccgtcg ccgttgatcg tgttgccgtc atcgcatttt tcgggacttt caagggtgcc
     2701 gttagggcag gtccccggtg tttcagcggc gcactcgggc gaacagccgt cgcccgccga
     2761 caggttgccg tcgtcacagc cctcgcccgg ctcggtgatg ccgttgccgc agacgatgcc
     2821 ctcgtcattc cgacaggcct gatagagcgg gcggcagaag atattgacgc attggctcag
     2881 ttgggtggcc gcaacgccgg cgcagttctg ctcgatgcat tcctgcaacg ggtcgaacag
     2941 cgtctgcgag ttgggcccgc cggcctccac gcacgtctct atgcaggtcc cgtcgccttc
     3001 gcaggtccgg ta
//
LOCUS       DAOQUE010000360         3004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_38297_length_3004_cov_3.563242, whole genome shotgun sequence.
ACCESSION   DAOQUE010000360 DAOQUE010000000
VERSION     DAOQUE010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38297_length_3004_cov_3.563242"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PKH10_10310"
     CDS             <1..203
                     /locus_tag="PKH10_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28556.1"
                     /translation="FVRAFPPPAMGHDTVLTREMISQWGMDKMPQWEFPPDKFAALDP
                     AFARRARGHRARPDDAAGERLA"
     gene            complement(360..1103)
                     /locus_tag="PKH10_10315"
     CDS             complement(360..1103)
                     /locus_tag="PKH10_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT28557.1"
                     /translation="MTAYIPALSNDSLSAYIHSALAVPDLGRDEEEDLFRRYRIEGDI
                     DAARRIILSSLKLVVHAAYKFRKFRDVVDLIQEGNVGLVTALKKFDLAKGVRFATYAL
                     WWIKAKIQEFILSHMSIVRYGKGRDERKLFFNLVSTVREIEAHDQGRELTREELIAEV
                     ARRLGMDPIRVADNMRVLTSICVALSGTTLASTANGLACRGSSLAAAAAPSAPGDSAR
                     AAGPAVHTTGIVPTMLPFCTLSVSPFLAP"
     gene            1324..1911
                     /locus_tag="PKH10_10320"
     CDS             1324..1911
                     /locus_tag="PKH10_10320"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNT28558.1"
                     /translation="MRHLLLSALFGLVLLLPPTAEARREKGFYFDDHLTVDNETLLKL
                     NGVGFLEKFSFTVYANTLYLKQPSNDWQAILDADEPMAMRLHILYSFIDLNELKEALR
                     DGFRYSTGRDPEKLKPLLARSEKLLSWFDRKTRKYDTATFLYVPGKGTMVSMNGTYKG
                     AIPGLDFKKALFGIWLCEKPANRPLKAGLLGLPEK"
     gene            complement(1915..2853)
                     /locus_tag="PKH10_10325"
     CDS             complement(1915..2853)
                     /locus_tag="PKH10_10325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT28559.1"
                     /translation="MSLLRDIPERELSDLLDEAQHLWETGRCCPTRCGVTRRWGVAAY
                     REPPCGGSSTGIRAASFAVHNGEEPPVSGWNGAGNIFFSGCNMRCIFCQNWPISHKNN
                     GRLYSLEEFAAEVRRLMRTGVHNLNLTTADHYLYPALKALADMRGEIPVPISYNCAGY
                     FTEEALSVVLRFADIFLYDVKYLDPALAARYSGAPNYVEAMTKGLEVLHAARIPWEED
                     ELGLLQRGLIIRHLVIPGAVDNSFRTLDLLAAYRDRGMIFKLSLMSQYFPAYRAVEHP
                     DISRRLTHDEYDPVVARMEELDLDGWTQDLDAHGGA"
     gene            complement(2855..>3004)
                     /locus_tag="PKH10_10330"
     CDS             complement(2855..>3004)
                     /locus_tag="PKH10_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HNT28560.1"
                     /translation="EGAVVAAGAVVTQHVEPYTVVAGVPARVIKKVDDRTKSKTELVN
                     ALRNL"
BASE COUNT          561 a          933 c          918 g          592 t
ORIGIN      
        1 gttttgtaag agcttttcct cctccggcca tgggccacga cacggtactg accagagaga
       61 tgatttcgca gtggggcatg gacaagatgc cgcagtggga gttccctccc gacaagtttg
      121 ccgcgctgga tcctgcgttc gcccgtagag cgcggggcca tcgtgcccgg cccgacgatg
      181 ctgccggtga gcggttggcg tgaggcagtg atcacgaacc acaacgcgaa cgcctgcgcg
      241 ccgtttccgg taaaggtcac cctgggtgcc agcctggtcg ccgacgccct ggtctggctg
      301 gagggagcac accccgacct gctacagggc gtcaccaaca aggaggggcg cattgacgtc
      361 tatggcgcca aaaacggtga cacgctgagg gtgcaaaagg gcaacatcgt tggcacaata
      421 ccggtggtgt gcacggccgg gccggcggct ctggccgagt cgccaggcgc agacggagcc
      481 gccgcggcgg cgagcgagct gcccctgcag gccaatccgt tcgcggtgga ggccagcgtg
      541 gttccgctca gcgccacgca gatcgaggtg agcacccgca tattgtcggc cacgcggatc
      601 gggtccatgc cgagccggcg cgccacctcg gcgatgagtt cctcgcgggt cagttcgcgc
      661 ccctgatcgt gggcttctat ctcgcgcacc gtcgagacga gattaaagaa cagtttccgt
      721 tcgtcacgcc ccttgccgta tctgactatg gacatgtgcg agaggatgaa ttcctgtatc
      781 ttcgccttta tccaccacag cgcataggtg gcgaagcgga cccccttggc gaggtcgaac
      841 ttcttgagcg cggtgacgag ccccacattc ccttcctgta tgaggtccac cacatcgcgg
      901 aacttgcgga atttgtaggc ggcgtgcacc accagtttga gactcgaaag aatgatgcgg
      961 cgcgccgcgt cgatatcgcc ttcaatgcga tagcgccgga acaggtcctc ctcttcgtcg
     1021 cgaccgaggt cgggcaccgc gagggccgaa tggatgtaag ccgacagcga gtcgttcgac
     1081 agggccggga tgtaagcggt catgatggta cctccttagc actcatcact ctcttctgct
     1141 aaaatgaaat accaaactcg ttgatatttg tcaagattgt tgttaataaa agttttatct
     1201 gtctgatttt cctcgcactc cggcgcagag aggacccttt cttttttatc ttggctatct
     1261 tttctgaata tgctatcctc gcgtcgggtc tttcgatggt cctgtcaccc gaacggaggc
     1321 accatgcgtc atctgctcct gtcggccctt ttcgggctcg tccttctgct cccccccacc
     1381 gccgaggcgc gccgggagaa gggcttctat ttcgacgatc acctcacggt cgacaacgag
     1441 acgctcctga aactcaacgg ggtcggcttc cttgagaagt tctccttcac cgtttacgct
     1501 aataccctct acctgaaaca gccatcgaac gattggcagg cgatactcga tgccgatgaa
     1561 ccgatggcga tgcggctcca tatcctttac agtttcatag acctcaatga actcaaggag
     1621 gcgctccgcg acgggttccg ttactcgacc ggacgcgacc ccgagaaact gaagccgctc
     1681 ctcgcgcggt ccgaaaaact cctctcgtgg ttcgaccgca agacgcgcaa gtacgacacc
     1741 gccacctttc tctatgtccc cggcaagggg acgatggtgt cgatgaacgg cacctacaag
     1801 ggggccatcc ccgggctcga cttcaaaaag gcgctgttcg gcatctggct gtgcgagaaa
     1861 ccggccaacc ggccgcttaa ggcgggactg ctcggtctgc cggagaagtg atattcacgc
     1921 gccgccgtgg gcgtcgaggt cctgcgtcca gccatcgaga tcgagttcct ccatccgcgc
     1981 gacgaccgga tcgtactcgt cgtgggtgag gcggcggctg atatcgggat gctccaccgc
     2041 gcggtaagcc ggaaaatact ggctcatgag cgacagtttg aagatcatcc ctctgtcgcg
     2101 gtaggcggcg agcaggtcga gcgtccggaa cgagttatcg accgcccccg gtatcacgag
     2161 gtgacggatg atgaggccgc gttgcaggag gccgagttcg tcctcctccc acgggatacg
     2221 ggccgcgtga agcacctcca gtcccttcgt catggcctcg acataattcg gagcgccact
     2281 gtatctcgcc gcgagcgccg ggtcgagata cttcacatcg tacaggaaga tgtcggcgaa
     2341 ccgcaggacg acggagagcg cttcttcggt gaagtagccc gcgcagttgt acgatatcgg
     2401 caccggtatc tcgccgcgca tgtcggcgag cgccttcagc gccgggtaga gatagtggtc
     2461 ggcggtcgtg aggttgaggt tgtggacccc cgtgcgcatg agacggcgca cctcggcggc
     2521 gaactcttcc agcgagtaga gccgtccgtt gttcttgtgc gatatcggcc agttttggca
     2581 gaagatgcac cgcatgttgc agccggagaa gaatatattc cccgcgccgt tccacccact
     2641 caccggcggc tcctcgccgt tgtggaccgc gaacgaggcg gcccggatgc cggtggacga
     2701 tccgccgcag ggcggctcac ggtaggcggc gacaccccac cgccgcgtca caccgcaccg
     2761 cgtcggacaa catcgtcccg tttcccagag gtgctgcgcc tcgtcgagaa ggtcggacaa
     2821 ttcccgttcg gggatatcgc gcaggagcga catgtcagag gtttcgcagg gcgttcacca
     2881 gttcggtctt cgacttggtc cggtcatcca ccttcttgat gacgcgcgcc gggaccccgg
     2941 cgacgacggt gtagggttcg acatgctggg tgacgaccgc accggccgcg acgaccgccc
     3001 cctc
//
LOCUS       DAOQUE010000361         3004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_38298_length_3004_cov_3.520855, whole genome shotgun sequence.
ACCESSION   DAOQUE010000361 DAOQUE010000000
VERSION     DAOQUE010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38298_length_3004_cov_3.520855"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PKH10_10335"
     CDS             <1..1082
                     /locus_tag="PKH10_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28561.1"
                     /translation="VQQTLRLRLFYKPVDWFSAETAWQVSPTVSQMGNDGYAALFTPS
                     LNDYRITDLPDTLYTGPGGQILFPHTIDRLNLTFTLPFMDLYAGRQAISFGAAKFVNP
                     TDVFAPLSFQDINREEKVGVDAVRVKFPIGEMNELDAGYVFGPDGRFSKSAAFLRGKF
                     ALWETDFTLMFVEYQYNLLAGLSVERAIGGAGVWLEAAYTADGVLEGRNSDDDNFRFS
                     AGADYNFADANLVLFGEYHYSMVGELYPSKYLTNTLTKPAYSDSGVYLLGAHYLAVGF
                     TWEIVPLLNGSFQTLTNLTDGSLLIAPRLEYSMADDLFLDVGASVGAGATARKGSFLG
                     FTYPVPRSEFGLYPYTVYTTVRLYF"
     gene            1124..2431
                     /locus_tag="PKH10_10340"
     CDS             1124..2431
                     /locus_tag="PKH10_10340"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT28562.1"
                     /translation="MRHLFFLAACVLVLSCDTLSLQERGSAGQPCFTDGTCLAPLTCV
                     EDVCALTGGGDNDNAQPDDQTGQSDDTTIPDDDTTTTDNDTSPDEDAATVDDYTSLDD
                     DSAPTDNDTEDPDVDTLPENACNEGEICWHIIDTAQTSCYNLTGSVSCPQEGSQFYGQ
                     DGNYSGGGREFQNRTTSDPTLIDDNQTKLVWQKATDGTARSWSDADAYCTGLAGSNYG
                     DRTDWRLPTRQELLSLLSFDAQSVEALIDGFYFPSTPADSFWTGTKHGYNYYYVDFSG
                     DGMSYSDAPETLHQVRCVSSPWEYLSERPITRWVETTAGTDTVIEDTLTGLVWQMAFT
                     YDERLWADALSYCAALNHAQKGDWRLPDINELESLITYGAEAEHVTTFPTIDEDYFWS
                     STTNAKDIAKAWVAEFKYGIIRPDMAKDVNSNGIRTICVRNKD"
     gene            complement(2434..>3004)
                     /locus_tag="PKH10_10345"
     CDS             complement(2434..>3004)
                     /locus_tag="PKH10_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019612895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HNT28563.1"
                     /translation="CHAILAAFLDSKPGDATAVALFYDNEEIGSRTPMGADSGFTATV
                     LERIVMAHDAGREEFHRALAKSFLVSSDSAHAIHPNFPEKHDPAYAPVMNGGPVIKVS
                     AGFKYATTAVTAERFRDLCRRAGVPCQKIANRSDVPSGSTIGPMTTAALSVPGVDVGV
                     PMWAMHSIRETAGTRDHLSLIAALKELFC"
BASE COUNT          587 a         1003 c          857 g          557 t
ORIGIN      
        1 cggtccagca gacactccgc ctgcgtctct tctacaaacc ggtcgactgg ttctctgccg
       61 agacggcatg gcaggtctcg ccgacggtgt cgcagatggg gaacgacggg tacgccgccc
      121 tcttcacgcc gtcgctgaac gattaccgca tcaccgacct ccccgacacc ctctacaccg
      181 gccccggcgg acagatactg ttcccccaca ccatcgaccg gctcaacctc accttcaccc
      241 tcccgttcat ggacctctac gccgggcggc aggcgatatc gttcggcgcg gcaaagttcg
      301 tgaatccgac cgatgtcttc gcgccgctct ccttccagga catcaaccgc gaggagaagg
      361 tcggcgtcga cgcggtccgc gtgaaattcc ccatcggcga gatgaacgaa ctcgacgccg
      421 gctatgtctt cggccccgac ggccgctttt cgaaaagcgc cgccttcctg cgaggcaaat
      481 tcgcgctctg ggagaccgac ttcacgctga tgttcgtcga gtatcagtac aacctgctgg
      541 cgggcctttc ggtagaacgg gccatcggcg gtgcgggtgt ctggctcgag gcggcataca
      601 ccgccgacgg cgttctggag ggacgcaaca gcgacgacga caacttccgc ttctccgccg
      661 gcgccgacta caacttcgcc gacgcgaacc tcgtcctgtt cggcgaatat cattactcga
      721 tggtcggcga actctacccg tcgaagtatc tcaccaatac cctgaccaag ccggcctata
      781 gcgatagcgg cgtctatctg ctcggcgccc attatctcgc ggtcggcttc acctgggaga
      841 tcgtgccact cctcaacggc tcattccaga cgctcaccaa tctgaccgat ggttcgctgc
      901 tcatcgcacc tcgccttgaa tacagcatgg ccgacgacct ctttctcgat gtcggcgcgt
      961 cggtcggagc cggggcgacg gcgcgcaaag gttcgttcct cggcttcacc taccccgtcc
     1021 cgcgctccga gttcggcctc tacccttaca ccgtctacac cacggtacgg ttgtatttct
     1081 gacatctttc tggtatagta gctcgttctt tcgtggaggc gccatgcgac acctgttttt
     1141 tctcgcggcc tgcgtactgg tcctttcctg cgatacgctt tcccttcagg agcgcggttc
     1201 tgccggtcaa ccctgtttca ccgacggcac ctgtcttgcg ccgctgacct gcgtcgagga
     1261 tgtctgcgca ctgaccggcg gcggcgataa tgataatgcc caaccggatg atcagaccgg
     1321 acagtccgac gacaccacga tccccgacga tgataccaca acgaccgaca acgacacgag
     1381 ccccgatgaa gacgcggcaa cggtcgacga ctacacgagc ctcgatgatg acagcgcacc
     1441 gaccgacaat gacacagaag atcccgatgt cgacactctc cccgagaacg cctgcaatga
     1501 aggcgagatc tgttggcaca tcatcgacac cgcccagacc agttgctaca atctgaccgg
     1561 atcggtctcc tgcccgcagg aaggaagcca gttctacgga caggacggca actactcggg
     1621 tggcggccgc gaattccaga accgtacgac ctcggatccg acgctcatcg acgacaatca
     1681 gaccaaactg gtctggcaga aagcgaccga cggcacggcc cgctcgtgga gcgacgccga
     1741 tgcctactgc accgggctcg cgggtagcaa ctacggcgac cgcaccgatt ggcgactccc
     1801 gacgcgacag gaactcctga gcctgctctc tttcgacgcg cagtcggtcg aggcgctgat
     1861 agacggtttc tatttcccct ccacccccgc cgacagcttc tggaccggca cgaaacacgg
     1921 ctacaactac tactatgtcg atttctcggg cgacggcatg tcttactccg atgcccccga
     1981 gacgctccat caggtccgct gcgtcagttc gccgtgggaa tacctgagcg aacggccgat
     2041 cacgcgctgg gtcgaaacga ccgccggtac cgataccgtc atcgaggaca ccctgaccgg
     2101 actggtctgg cagatggcgt tcacctacga tgaacggctc tgggccgatg cgttgagtta
     2161 ctgtgcggca cttaaccatg cccagaaagg cgattggcgg ctccccgaca tcaacgaact
     2221 ggagagtctc atcacctacg gggctgaggc ggaacatgtc accaccttcc cgacgataga
     2281 tgaggactat ttctggagtt cgaccaccaa cgcgaaggat atcgcgaagg cgtgggtcgc
     2341 cgaattcaag tacggcatca tacggcccga tatggcgaaa gatgtcaaca gcaacggcat
     2401 ccgcacgatc tgtgtgcgga acaaggactg aatctagcag aacaattcct tcagcgcggc
     2461 gatgagcgaa agatgatcgc gcgttcccgc cgtttcgcgg atgctgtgca tcgcccacat
     2521 cggcaccccg acatcgaccc ccggcaccga cagggcggcg gtggtcatcg ggccgatggt
     2581 gctccccgac gggacatcgg agcggttcgc tatcttctgg cacggcaccc cggcgcggcg
     2641 gcatagatcg cggaaccgtt ccgccgtgac ggcggtcgtc gcgtacttga acccggcgct
     2701 caccttgatc accgggccgc cgttcatgac cggcgcatag gccggatcat gcttttcggg
     2761 aaagttgggg tgtatcgcgt gggccgaatc ggatgagacg aggaacgact tggcgagcgc
     2821 ccggtggaac tcctcgcggc cggcgtcgtg cgccatgacg atccgttcaa ggaccgtcgc
     2881 ggtgaacccg ctgtcggccc ccatcggggt gcgcgaaccg atctcctcgt tgtcgtagaa
     2941 cagcgccacg gcggtcgcgt cgcccggctt tgaatcgagg aacgcggcga gtatcgcatg
     3001 gcac
//
LOCUS       DAOQUE010000362         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_38315_length_3003_cov_3.895183, whole genome shotgun sequence.
ACCESSION   DAOQUE010000362 DAOQUE010000000
VERSION     DAOQUE010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38315_length_3003_cov_3.895183"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKH10_10350"
     CDS             <1..464
                     /locus_tag="PKH10_10350"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNT28564.1"
                     /translation="MRRSRTTTTRYDHDDVVEIFIDANGDRKDYWEMQVSAAGVKFDA
                     SFKGGPRKNMDVAWDSGIKYAVQVNGTLNKPDDADKGWTAEIAIPWKSIADAVNNPPK
                     DGDIWKAYLYRIDRNGSGKRPEFSAWVPPYKGDFHYLRFMGDLVFVYEEIL"
     gene            448..1272
                     /locus_tag="PKH10_10355"
     CDS             448..1272
                     /locus_tag="PKH10_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28565.1"
                     /translation="MKRYSRRLLPAAILFLFFVLRSAQAPDRAADNRHKILSARLQAE
                     VAQVERRLAPAGSRHEAAARKYLTNLAAHKKLQLLRRSSARLRERCFWFDGECRHLYG
                     MTIVYLDSEIALLQERMAADREEAIAAAEDHAALEERYRYLTQTDLPIPDPGAPEGYE
                     IPELRGLYRCVNVEGWNPLRGVFVSGDQPLDLPFPVEATTVYRERGSVFVSLKFRDAI
                     LQYAYTGDPVFFEGEIVPPYTTVAEKATGNPIRPGTVIVSIVRKGRFVDPSFICRR"
     gene            1287..>3003
                     /locus_tag="PKH10_10360"
     CDS             1287..>3003
                     /locus_tag="PKH10_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28566.1"
                     /translation="MRALLFSVILAIAPFLSAATVQGHFTATDGGTPAIRLVMAFDAA
                     DSSQLGDMTTDATGAYSFDIADDTTVMLIGFASRGKTLEGYTLHEYTPAVKLIPAVSG
                     TVTADLSTAPARHFIFEAKMDGVPVNTGSFTNAFPFDGDDRAIPTFSIGAEKSGGTGQ
                     VPSYNIALGGFVSFFVEWELPEAGRIMVRIDNGGDGYPLEEQGGVILDLNREMAFSAL
                     ERLRRMIGTEVARYPDAAEALAAAQALFDGEQYDESAGTAIVAAEDLAVELSRRAIDE
                     ARKGTITVTVTDADGRPIPGATVDIAQTSRDFLFGALPNIPHGGEEFMRMLYDDGITF
                     ATAGVLWKDIEPTDDDYSWYAPDSWFGVPLLDDMGFTLTGHSLVYFLDRIMPEYLMTL
                     GGDDLLAEVEEHTAMVVGHYQPVMDEWILINEAHTYQASRGLSREDITAVTRTAYDTV
                     RETAPGVPTMVNAAPDWFGQSVVTELLLPEREGFFTLPPKRYFEELYQNVPFDRIGQQ
                     MYNGGCVTLFADNGMGDVGVMPVYDLAELRRQLSSLAELGLPMHLSKVSVPSAMPAAC
                     AGMGYW"
BASE COUNT          565 a         1019 c          916 g          503 t
ORIGIN      
        1 ccatgcgacg ctcaaggaca acgacgacaa gatacgatca cgacgatgtg gtcgagatat
       61 tcatagacgc gaacggcgac cggaaggatt actgggagat gcaggtcagc gccgccggcg
      121 tcaagttcga cgcatcgttc aaaggcggcc cgcgcaagaa catggatgtc gcgtgggatt
      181 cgggcatcaa gtacgcggtg caggtcaacg gaacgctcaa caagcccgac gacgccgaca
      241 agggctggac cgccgagatc gccatcccgt ggaaaagcat cgccgatgcg gtcaacaacc
      301 cgccgaagga cggcgacatc tggaaagcgt acctctaccg gatagaccgg aacggaagcg
      361 ggaaaagacc ggaattctcg gcctgggtgc cgccctacaa gggcgatttc cattacctgc
      421 ggttcatggg ggatctcgtt tttgtctatg aagagatact ctaggcggct tctccccgcc
      481 gccatccttt ttctgttctt cgtcctgcgg tcggcgcagg cccccgaccg cgccgccgac
      541 aaccgccaca agatactttc ggcccgtctt caggccgaag tggctcaggt cgaacgccgg
      601 ctcgcccccg ccgggagccg acatgaggcg gcggcgcgga agtacctgac caatctcgcg
      661 gcgcacaaga aactgcaact gctccgccgt tcgtccgccc ggctccgtga acggtgcttc
      721 tggttcgacg gggagtgccg acacctctac ggcatgacca tcgtctatct cgacagcgag
      781 atcgctctcc tgcaggagcg gatggccgcc gaccgcgaag aggccatcgc cgccgccgag
      841 gatcatgcgg cgctggagga acggtaccgc tatctcaccc agaccgacct gccgatcccc
      901 gatccgggag caccggaggg atacgagata cccgaactgc gcggactgta ccgctgcgtg
      961 aatgtggagg gatggaaccc gctccggggc gtgttcgtga gcggcgatca gccgctggac
     1021 ctccccttcc ccgtcgaggc gacgaccgtg taccgcgaac ggggctccgt gttcgtatca
     1081 ctgaagttcc gggacgccat cctgcagtac gcctacaccg gcgaccccgt ctttttcgag
     1141 ggcgagatcg tccctcccta caccaccgtc gctgaaaagg cgaccggcaa tccgatacgc
     1201 cccggcaccg tcatcgtcag tatcgttcgc aagggccggt tcgtcgaccc ttcgttcatc
     1261 tgtcgtcgct gatacgggag gtccccatgc gcgccctgct tttttcggtc atcctcgcca
     1321 tcgctccctt cctttcggcc gccacggtgc agggccactt cacggcgacc gacggcggca
     1381 cgcccgccat acggctcgtc atggcgttcg acgccgccga ttcctcgcaa ctcggcgaca
     1441 tgacgaccga cgcgacgggc gcctactcgt tcgacatcgc cgatgacacc accgtcatgc
     1501 tcatcgggtt cgcctcgcga ggcaagacct tggaaggcta cacgctccac gaatacaccc
     1561 ccgccgtgaa actcataccg gcggtatcgg gcacggtgac ggccgacctc tcgaccgctc
     1621 cggcgcgcca tttcatattc gaggcgaaaa tggacggggt gcccgtgaac accgggtcct
     1681 tcaccaacgc cttccccttc gacggcgacg accgcgccat acccaccttc agcatcggcg
     1741 ccgaaaagag cggcgggacc ggtcaggtcc cctcctacaa catcgcgctc ggcggcttcg
     1801 tatccttctt cgtcgaatgg gaactgcccg aggcgggacg catcatggtg cgcatcgaca
     1861 acggcggcga cggctacccg ctcgaagagc aggggggcgt gatactcgac ctcaaccggg
     1921 agatggcctt ctcggccctc gaacggctcc ggcggatgat cgggacggag gtggcgcgct
     1981 accccgacgc ggccgaggcg ctcgcggcgg cacaggcgct tttcgacggc gaacagtacg
     2041 acgagtcggc gggcacggcg atagtagcgg cggaggatct cgccgtcgaa ctgtcgcgca
     2101 gggccatcga cgaggcgcgc aagggcacga tcaccgtgac ggtcaccgac gcggacggtc
     2161 gcccgatccc cggcgcgacg gtcgacatcg cgcagacatc gcgcgatttc cttttcggcg
     2221 cacttccgaa cataccgcac ggcggcgagg agttcatgcg gatgctctac gatgacggca
     2281 tcaccttcgc gaccgccggc gtcctgtgga aggacatcga gccgaccgac gacgactatt
     2341 cgtggtacgc ccccgactcg tggttcggcg tcccgctgct cgacgacatg gggttcaccc
     2401 tcaccggcca ctcgctcgtc tacttcctcg accgcatcat gcccgaatat ctgatgacgc
     2461 tcggtggcga cgatctgctc gcggaggtcg aggagcacac cgccatggtg gtcggccact
     2521 accagccggt gatggatgaa tggatactca tcaacgaggc gcacacctat caggcatcac
     2581 gcggcctttc gcgggaagat atcaccgccg tcaccagaac ggcctacgac accgtccgcg
     2641 aaacggcacc gggcgtcccg acgatggtga acgccgcccc cgactggttc ggtcagagcg
     2701 tcgtcaccga actcctgctc cccgaacggg aagggttctt cacccttccg ccgaaacgct
     2761 atttcgagga actataccaa aacgtcccgt tcgaccggat cggtcagcag atgtacaacg
     2821 gcggctgcgt aacgctcttc gccgataatg gcatggggga tgtcggggtc atgccggtct
     2881 acgatctcgc cgaactgcgg cggcaactct cgtcgctcgc cgaactcgga ctgccgatgc
     2941 acctctcgaa ggtcagcgtg ccgtcggcga tgccggccgc ctgcgccggg atgggttact
     3001 ggc
//
LOCUS       DAOQUE010000363         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_38318_length_3003_cov_3.267300, whole genome shotgun sequence.
ACCESSION   DAOQUE010000363 DAOQUE010000000
VERSION     DAOQUE010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38318_length_3003_cov_3.267300"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3003)
                     /locus_tag="PKH10_10365"
     CDS             complement(<1..>3003)
                     /locus_tag="PKH10_10365"
                     /inference="COORDINATES: protein motif:HMM:NF025125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNT28567.1"
                     /translation="NALDQLIITVSVIEELDGAPVVTLDEAPLDDPTPEGNSWIYAPA
                     LTHGDHTIKATATDTAGNTTAAPIVKTYSVDAQYPTATVSEPDPDRISGSGKASVTVT
                     GGAGETLVHVKLNGNDCAGTGPYTCDFDAPAGSGDTTETLVVSFSDEAGNTTTVTKTV
                     TVDRTAPVLTFKKNKDGIYNKNDIGQILLSISSTEELSELTVNGTTQNDPTGLSWDIT
                     PDTSTNTHTIIATAKDKAGNPTAVALEESYTIDATDPTATIGALSATRVKENTEVTFT
                     VTKAGEEDLASVTVRDSDGTLKDCPDGDCAYTTAVGTVGTDTTETVTVQYTDAAGNTV
                     IVPAGTVHVDRTAPVLTFKKNKNTIYNKNDIGQILLSISSTEELSELTINGNTQADPA
                     GLSWDIPPDTSDGPHPVTATAKDKAGNPTAAALEDGYAVDMTDPEATIGALSATRVQG
                     NTTVTFTVTKAGEEDLASVMVRDSDGTLKDCPDGDCAYTTAVGTVGTDTTETVTVQYA
                     DAAGNTVTANAGTVYVDRTAPRMVGTLISPKEARKDALVQVVVTFDEPVSGANFGGDG
                     LAFTRTDDGSDPQRYTYEYPVAGTETKESYALTLAAVDAANNPVADTGIGILTIDLLA
                     PTVNDLLVCHDDTTDDPCAVDETLFSAVTDHREMKISFSLSEAADTLSVRLGTTELTA
                     TNCTAFAGPSYICTATVMDAAAGTDPRMAGDLATVSTGDDAGNSDFDSKSVVYDFLPP
                     SVAGDATIVLTRPDDCPLLSDELARVTYGTTVRAQFSVSEELDGDEPVSVIAQKDTQT
                     FPLDPDANAGYSFTYLMTMPLSGTFPQGDYVLAVTLRDTVGNEAELSPTLTAPGIYLD
                     TAAPADPDVATADRIVYRRIPWGSDATGGVKSFSISAGTDAVDATTAFVIAFDGDDPA
                     TDAQIGIGPVTNRSFADMELNRADRAEVYLAAYDTACNRSDTVRVRDVEWTATMGYKV
                     AGSTFENPNRYKKQKWFTDTLDQYGAVE"
BASE COUNT          429 a          896 c          997 g          681 t
ORIGIN      
        1 gttccacggc cccgtactgg tccagcgtgt cggtgaacca tttctgtttt ttgtagcggt
       61 tcgggttctc gaaggtgctt cccgcgacct tgtaccccat cgtggcggtc cattcgacat
      121 cgcgcactct gacggtgtcg ctccggttgc aggcggtatc gtaggccgcg agatagacct
      181 cggcccggtc ggcgcggttc agttccatat cggcgaaaga gcggttcgtc accggtccga
      241 tacctatctg ggcgtcggtc gccgggtcgt cgccgtcgaa ggcgatcacg aaggcggtcg
      301 tcgcgtcgac cgcgtcggta cccgccgaga tgctgaaaga cttcaccccc cctgtggcgt
      361 cactccccca cgggatgcgc cggtacacga tcctgtccgc cgtcgcgaca tcggggtcgg
      421 cgggcgcggc ggtgtcgaga tagatccccg gcgcggtgag tgtcggagat agctcggcct
      481 cgttgccgac cgtgtcgcgg agcgttacgg caagcacgta atcgccctgc gggaaggtgc
      541 cgctgagcgg catcgtcatc aggtaggtga aggaataccc ggcgttcgca tccgggtcca
      601 gcgggaaggt ctgcgtatct ttttgagcga tgaccgatac cggttcatcg ccgtccagtt
      661 cctcgctcac cgagaactgc gccctgacgg tcgtgccgta ggtgacccgc gcgagttcgt
      721 cggaaaggag cggacaatcg tccggtcggg tcagcacgat agtggcgtcg ccggccacgc
      781 tgggtggcag gaaatcgtaa acgacgcttt tcgagtcgaa gtcgctgttc ccggcgtcat
      841 cgccggtact taccgtggcg agatctcccg ccatccgcgg gtcggtcccg gcggctgcgt
      901 ccatgacggt cgcggtgcag atatacgacg gtcccgcgaa ggcggtgcag ttcgtggcgg
      961 tcagttcggt cgtgccgagt cgcaccgaaa gggtgtcggc cgcctcgctc aaggagaagg
     1021 atatcttcat ctccctgtgg tccgtgacgg cgctgaacag cgtttcatcg accgcgcacg
     1081 ggtcatcggt cgtatcatca tgacagacga gaaggtcgtt taccgtcggg gcgaggagat
     1141 cgatggtaag aatgccgatg ccggtgtcgg cgaccggatt gttcgcggca tcgacggccg
     1201 caagggtcag ggcgtaactc tccttggtct cggtgcctgc caccggatac tcataggtgt
     1261 atcgctgagg gtcgctcccg tcgtcggtcc gcgtgaaggc aagtccgtcg ccgccgaaat
     1321 tagcgccgct caccggctcg tcaaaggtga cgacgacctg taccagggcg tcttttctcg
     1381 cctctttcgg ggagatgagt gtgcccacca tgcgtggcgc ggtgcgatcc acgtagacgg
     1441 tcccggcgtt cgcggtcacc gtgttgcccg ccgcgtcggc atactggacg gtaacggtct
     1501 ccgtagtgtc ggtgccgacg gtgccgaccg ccgtggtgta ggcgcagtcg ccgtccggac
     1561 agtccttcag cgtgccgtcg ctgtcacgta ccataacgga ggcaagatcc tcctctcccg
     1621 cttttgtcac ggtgaaggtg acggtcgtgt tcccctgcac ccgcgtcgcc gagagggctc
     1681 ctatcgttgc ctccggatcg gtcatgtcga cggcgtatcc gtcttccagc gccgcggcgg
     1741 tcgggttacc cgccttgtcc ttcgccgtcg cggtgacggg gtggggtccg tcgctcgtat
     1801 cgggaggtat gtcccaactg aggcccgccg ggtccgcctg ggtattcccg ttgatggtca
     1861 gttcggatag ttcttcggtg ctcgatatgc tcagcaggat ctgaccgatg tcgttcttgt
     1921 tgtagatcgt attcttgttc ttcttgaagg tcagcaccgg cgcggtccta tcgacatgaa
     1981 cggtgcccgc cggaacgatg acggtgttgc ccgccgcgtc ggtatactgg acggtaacgg
     2041 tctccgtagt gtcggtgccg acggtaccga ccgccgtggt gtaggcgcag tcgccgtccg
     2101 gacagtcctt tagcgtgccg tcgctgtctc gcaccgtaac ggaggcaaga tcctcctctc
     2161 ccgcttttgt cacggtgaag gtcacctcgg tgttctcctt cacccgtgtc gccgaaagcg
     2221 cccctatcgt cgctgtcggg tcggtcgcgt caatggtgta actctcttcc aacgctacgg
     2281 cggtcgggtt gcccgccttg tctttcgccg tcgcgatgat ggtatgagtg ttcgtgctcg
     2341 tgtcgggagt gatatcccaa ctgagtcctg tcgggtcgtt ctgcgtggtg ccgttcaccg
     2401 tcagttcgga aagctcttcg gtgcttgata tgctcagcag gatctgaccg atgtcgttct
     2461 tgttatagat gccatccttg ttcttcttga atgtcagcac cggtgcggta cggtcgaccg
     2521 tcacggtctt ggtgacggtc gtggtgttgc ccgcctcgtc gctgaacgat acgacgagcg
     2581 tttcggtcgt gtcgccgcta ccggctgggg cgtcgaagtc gcaggtgtag gggccggtgc
     2641 cggcgcagtc attaccgttg agttttacat gcaccaaggt ctcgcctgcc cctccggtca
     2701 ccgtcacgga cgccttgccg ctccccgata tcctgtcggg gtccggttcc gatacggtcg
     2761 cggtggggta ctgggcgtcg acgctgtagg tcttgacgat aggggcggcg gtggtgttgc
     2821 cggcggtgtc ggtggccgtc gctttgatgg tgtgatcccc gtgggtcaac gccggggcgt
     2881 atatccagga gttcccttcc ggtgtcgggt cgtcgagagg tgcctcgtca agggtcacga
     2941 ccggggcgcc gtcaagttct tctatgacgc tgacggtgat gatgagttgg tcaagggcat
     3001 tgt
//
LOCUS       DAOQUE010000364         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_38319_length_3003_cov_3.250678, whole genome shotgun sequence.
ACCESSION   DAOQUE010000364 DAOQUE010000000
VERSION     DAOQUE010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38319_length_3003_cov_3.250678"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PKH10_10370"
     CDS             <1..405
                     /locus_tag="PKH10_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28568.1"
                     /translation="LAPEGNLGLGREAIALAAWRIDPGTCTAIDHGTIHASLISYATS
                     KLGLKLSPAPTTADLDSLTKDPATARFLTARLSCSKRGDLFVAAVSVELYDLFEQALI
                     HSAIAEGVSDADWRGALLQAETALAPFKPSAP"
     gene            413..1045
                     /locus_tag="PKH10_10375"
     CDS             413..1045
                     /locus_tag="PKH10_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT28569.1"
                     /translation="MSFRVVTEPIGPVAANCHTLEFGDQVILIDPGGDADLLLRLIGT
                     RTLTAMLVTHGHYDHIGAIGELKERHPGAAVMIHRDERLFLSDPSLNLSCHFGIDGRY
                     GHAPERLLDHGDRIMLPDGDTLSVIHTPGHTPGGVCFYGDGVLFSGDTLFFESIGRCD
                     LPGGDEETLLTSIRERLLTLPDDTRVLSGHMNVTTIGHEKRHNPFLDGAV"
     gene            1118..1549
                     /locus_tag="PKH10_10380"
     CDS             1118..1549
                     /locus_tag="PKH10_10380"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT28570.1"
                     /translation="MVISGRRTSKERRDGDQAHFPKDDRRKERRGSERRTKDRIPVKM
                     WIRNIDGEASYFQQTGNLSTSGMYILSPAPHEKGTRITLEFQIPASTRVVRCTAEVVN
                     CRADGQFWGVSVKFIDVSKQDKAEIEKAVSNLLSEYWYLVE"
     gene            1549..>3003
                     /gene="nadB"
                     /locus_tag="PKH10_10385"
     CDS             1549..>3003
                     /gene="nadB"
                     /locus_tag="PKH10_10385"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNT28571.1"
                     /translation="MSPRRTDVLVIGSGLSGLIASLLLARKGLRVLIVTKDEIDASNS
                     HLAQGGIATVADLAHDSFQKHRDDTIAAGDGLNDPAAVDFFVQRGPDIVAFLRDMGVQ
                     FSTRANGDLDLGWEGGHTERRVVHAGDITGNELIGVLKRAVFREHLITIWQHHTAIDI
                     ISTRKFLLANEDRAVGAYVLSHEGTVVTVEARAVLLATGGCGRVYLYTSNPDVATGDG
                     VAIGFRAGIPAVNMEMIQFHPTILYHEKVRSMLLSEALRGEGGVLKTRDGNTFMERYH
                     PLRSLAPRDVTARAIDTELKKRGDDFVLLDMTMLDREFIVNRFPNLFKKCLDAGIDMR
                     EDPIPVVPAAHYMCGGLRTTPEGDTCVPGLFIAGETAHTGLHGANRLASNSLLEAAIM
                     ARCAADGIARWIGDAPVVPAALPPWNEEGVSDSDEQVVIKQNWDEIRHCMWNYVGIVR
                     SDKRLERALKRVSLMKQEIDDYYWNFKVNRDLLEL"
BASE COUNT          600 a         1007 c          881 g          515 t
ORIGIN      
        1 ctcgcccccg agggcaacct ggggctcggc agggaggcga tcgccctcgc cgcgtggcgc
       61 atcgacccgg gcacctgcac cgcgatcgac cacggaacta tccatgcctc cctgatctca
      121 tacgccacca gcaaactggg gctgaaactc tctcccgcgc cgacgaccgc tgacctcgat
      181 tcgctcacga aggaccccgc aacggcacga ttcctcaccg cccgactttc ctgctccaag
      241 cgcggcgacc tcttcgtcgc cgccgtctca gtcgaactgt acgacctctt cgaacaggcg
      301 ctcatccatt cggccatcgc cgaaggggtc tccgacgccg actggcgcgg cgcactccta
      361 caggccgaaa cggcgctcgc ccctttcaaa ccgtcggcgc cctaggaacg ccatgtcctt
      421 ccgcgtagtc accgaaccga tcggcccggt cgccgccaac tgtcataccc tcgaattcgg
      481 cgatcaggtc atcctgatag acccgggcgg cgatgccgac ctactgctcc ggctcatcgg
      541 gacgcgtacg ctgaccgcga tgcttgtaac gcatggtcac tacgatcaca tcggagccat
      601 cggcgaactc aaggaacgcc accccggtgc cgccgtcatg atacaccgtg acgaacggct
      661 cttcctctct gatccatcgc tgaatctctc ctgtcatttc ggtatcgacg gacgctacgg
      721 acacgcaccg gaacgcctgc tcgaccatgg cgaccgcatc atgctccccg acggcgacac
      781 gctctcggtc atccacacgc cggggcatac tcccggcggt gtctgttttt atggcgacgg
      841 tgtgctcttc tcgggcgaca cgctcttttt cgagagcatc gggagatgcg acctcccggg
      901 cggcgacgag gagacactgc ttacgagcat acgggaacga cttctcacgc ttcccgacga
      961 cacgcgggtc ctgtcgggtc acatgaacgt aacgaccatc ggccacgaga aacgacacaa
     1021 cccgttcctc gacggtgccg tctgaaaata agtggacaaa aagagggggc ttcgctatag
     1081 tgggcccggt atcgttcact agggcatgga ggagagcatg gtgatcagcg gcagacggac
     1141 cagcaaggag cgccgcgacg gcgatcaggc gcattttccc aaggatgacc gtcgcaagga
     1201 acggcgcggc tccgaacggc gcaccaagga ccgcatcccc gtcaagatgt ggatacgcaa
     1261 catcgacggt gaggcgagtt atttccaaca gacgggcaat ctcagcacga gcggcatgta
     1321 tatcctctcc ccggcgcccc acgaaaaagg gacccgcatc acgctcgaat tccagatacc
     1381 ggcgagcacc cgcgtggtgc gctgtaccgc cgaggtggtc aactgccgtg ccgacggcca
     1441 gttctggggc gtcagcgtca agttcatcga tgtttccaag caggataagg ccgagataga
     1501 gaaggcggtc agcaacctcc tctctgaata ctggtatctg gtggaataat gtctccgcga
     1561 cggaccgatg tcctcgtcat cggcagcgga ctgtcgggac tcatcgcgtc acttctcctc
     1621 gcccgtaaag gtctccgggt actcatcgtc accaaggatg agatcgatgc gtcgaacagt
     1681 catctcgcgc agggcggcat cgcgaccgtc gccgatctcg cgcacgacag ttttcagaaa
     1741 caccgggacg acaccatcgc cgcgggcgac ggcctgaacg atccggcggc cgtcgatttc
     1801 ttcgtgcaac gcggccccga catcgtcgcg ttcctccgcg atatgggggt acagttctcg
     1861 acccgcgcca acggcgacct cgacctcggt tgggagggcg gccacaccga acgccgcgtg
     1921 gtgcacgcgg gtgacataac cggcaacgaa ctgatcgggg tgctcaaacg ggcggtgttc
     1981 cgcgagcatc tcatcaccat ctggcagcac cacaccgcga tagatatcat ctcgacgcgc
     2041 aaattcctgc tcgcgaacga ggaccgcgcc gtcggcgcct atgtgttgtc acacgaaggg
     2101 acggtcgtca ccgtcgaggc gcgtgcggtc ctgctcgcga ccggcggctg cggcagggtc
     2161 tatctctaca cctcgaaccc cgatgtggcc accggtgacg gggttgccat cgggttccgc
     2221 gccggcattc cggcggtcaa catggagatg atacagttcc acccgacgat actgtatcac
     2281 gagaaggtcc ggtcgatgct cctttcggag gcgcttcgcg gcgaaggcgg cgtcctgaag
     2341 acccgcgacg ggaacacctt catggaacgc taccacccgc tccgctcact cgctccccgc
     2401 gatgtgacgg ctcgcgccat agacaccgaa ctcaagaagc gcggggacga tttcgtcctg
     2461 ctcgacatga cgatgctcga ccgcgagttc atagtgaacc gtttcccgaa cctgttcaaa
     2521 aaatgtctcg acgccggcat cgatatgcgt gaggacccga taccggtcgt ccccgccgcc
     2581 cactacatgt gcggcggtct gcgcaccacg ccggaaggcg atacctgcgt accgggcctc
     2641 ttcatcgcgg gagagaccgc tcacaccggc ctgcacggcg ccaaccggct cgcctcgaac
     2701 agtctgctcg aagcggcgat catggcgcgt tgcgcagccg acggcatcgc gcgatggatc
     2761 ggcgacgcac ccgtcgttcc ggcggcactg ccgccctgga acgaggaggg cgtcagcgac
     2821 agcgacgagc aggtcgtcat caaacagaac tgggacgaga tacgccactg tatgtggaac
     2881 tatgtcggca tcgtgcggag cgacaagcga cttgagcggg cactcaagcg ggtctcgctc
     2941 atgaagcagg agatcgacga ctactactgg aacttcaagg tcaaccgcga tctgcttgaa
     3001 ctg
//
LOCUS       DAOQUE010000365         2997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_38429_length_2997_cov_3.833447, whole genome shotgun sequence.
ACCESSION   DAOQUE010000365 DAOQUE010000000
VERSION     DAOQUE010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38429_length_2997_cov_3.833447"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /gene="rodA"
                     /locus_tag="PKH10_10390"
     CDS             <1..1058
                     /gene="rodA"
                     /locus_tag="PKH10_10390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNT28572.1"
                     /translation="LVGILLLGLVTLSSALSYNPQRLYAQLVWVALAAAAFLFVTYRP
                     LRMWENLAVPFYLAALLLLSLVLVIGKKVGGAYRWLDLGFMNLQPSEFAKVAVILFLA
                     ARLNQKPTLQDGYKLADIVPETVAVLLTMTLIYAEPDLGTSLLIAFVALMVILSTKIN
                     TRHLIALIIITVVSTPILWNFVLADYQKRRITAVATMVFSGASEESLSSRYQTEQSVI
                     AVGSGRMSGKGYQAGTQNMFRFIPEHHTDFVFSVYAEEFGFAGGMLLFAFYLLLFYRM
                     LEIVPLVRDKFMALVTVGTVVVLWLQVIINIGMVTGMLPVVGIPLPFFSYGGSALLAN
                     SILVGLVHNFSINSRYR"
     gene            complement(1111..1323)
                     /locus_tag="PKH10_10395"
     CDS             complement(1111..1323)
                     /locus_tag="PKH10_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNT28573.1"
                     /translation="MPIYEYLCAGCGKHLEVTQKMSDAPLTTCESCGGKLEKQVSLGA
                     IVTKGDAPSCPAGTKSCCPGCTHNHH"
     gene            complement(1377..1928)
                     /locus_tag="PKH10_10400"
     CDS             complement(1377..1928)
                     /locus_tag="PKH10_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28574.1"
                     /translation="MGWDIDLGDILRRLIGKPRILKPTPVPPMPPDWKLTYADLVDEA
                     KAGKRASIGRAEMAWAKEYERGLIPAGMRFPQKGDLYESLADQTIEYITAWAAPYSGG
                     GTGTLFKGEQVWIHSAPRDEKPIGEYALPVEYKKVEERMVPEQERTSSKYGGFSFYFT
                     TIELNTNFKLVGTEFKGKKGGTH"
     gene            complement(1912..2562)
                     /locus_tag="PKH10_10405"
     CDS             complement(1912..2562)
                     /locus_tag="PKH10_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNT28575.1"
                     /translation="MELEITQIGKECHGAGCSVFQRVGECLMPKQGLFARVITPGIVK
                     VGEKAELIERTLNVHVVTLSDRAATGDYEDRSGPRIAEMIAAHFTGTRWHLAIDRTVI
                     PDEPEMLAKILERDRDEATDVVVTTGGTGIGPRDITPEVILEIADREIPGIMEHIRVK
                     YGTDKPSALLSRSVAAVMGDTIIYALPGSVRAVEEYMAEILKTLEHAILMRHGLGH"
     gene            2524..>2997
                     /locus_tag="PKH10_10410"
     CDS             2524..>2997
                     /locus_tag="PKH10_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28576.1"
                     /translation="MAFLAYLRYLQFHRSRDEAVERCGAQEVDARTDVYAMCTVLYEF
                     LTLNYYLGNVGEEMSDLVGAILNRKPPDAESWSDPLNGRVPRALSRILRKGLSKKRVD
                     RFQNAGELEEVLQDWMEGNAPVVCPGTAMQRGLSRYSRWIDRHPVFVPAFTIILGI"
BASE COUNT          497 a          913 c          919 g          668 t
ORIGIN      
        1 cgctcgtcgg catcctcttg ctggggctcg tgaccctgtc gagcgccctt tcctacaacc
       61 cccaaaggct ctatgcccaa cttgtctggg tggcgctcgc cgccgccgcc tttttgttcg
      121 tgacctatcg acctctgcgg atgtgggaga acctcgcggt cccgttctat cttgccgcct
      181 tgctcctatt gtcgctcgtc cttgtgatcg gcaaaaaggt cgggggtgcc taccgctggc
      241 tcgacctcgg cttcatgaac ctacagccct ccgagttcgc caaggtcgcg gtcatcctgt
      301 tccttgcggc gcgccttaat cagaaaccga ccctgcagga cggctacaaa ctggccgaca
      361 tcgtgcccga gacggtcgcg gtcctgctta ccatgacgct tatctacgcc gagccggacc
      421 ttggtaccag cctgctcata gcattcgtgg ctctcatggt catcctttcg acgaagataa
      481 acacccgtca tctcatcgcg cttatcatta tcaccgtcgt ttcgacgccg atactctgga
      541 atttcgtgct cgcggattac cagaaacggc gtatcaccgc cgtcgcgacg atggtgttca
      601 gcggcgcatc ggaggaaagt ctgtccagcc gctaccagac cgaacagtcg gtcatcgcgg
      661 tggggtcggg gagaatgagc ggcaaggggt atcaggccgg cacgcagaac atgttccgtt
      721 tcatcccgga gcaccacacc gatttcgtct tttcggtata cgccgaggag ttcgggttcg
      781 ccggcggcat gctgcttttc gcattctatc tgctcctgtt ctataggatg ctcgagatcg
      841 tgccgctcgt tcgcgacaag ttcatggcgc tcgtgacggt gggtaccgtt gtcgtgctct
      901 ggttgcaggt catcataaac atcggcatgg tgaccggcat gttgccggtc gtcggcatcc
      961 cgctcccgtt cttttcgtat ggcggctcgg cgctactcgc caacagcata cttgtcgggc
     1021 tcgtgcacaa tttcagcata aacagcaggt accgataaac cccgtcaagc cccccttgac
     1081 aagggggcgg aaggatgggc tggatggatt ttagtggtgg ttgtgggtac agccgggaca
     1141 gcaggatttc gtgccggcgg ggcatgaggg ggcgtcgcct ttggtgacga tggcgccgag
     1201 cgacacctgc ttttcgagtt taccgccgca tgactcacag gtggtgagcg gcgcgtcgga
     1261 catcttctgc gtcacctcga gatgtttgcc gcatccggcg cacagatatt catagatcgg
     1321 catcgcgttc ctccttcaaa gacatcgtct ttgttatagc gacgacagat cggttgtcaa
     1381 tgtgtgccac ctttctttcc cttgaattcg gtgccgacca gtttgaagtt ggtgttgagt
     1441 tcgatggttg tgaagtagaa cgagaagccg ccgtacttgg acgaggtccg ttcctgttcg
     1501 gggaccatcc gttcttcgac ctttttgtac tcgaccggca gggcgtattc gccgatgggc
     1561 ttttcgtcgc gcggtgcgga gtgtatccag acctgctccc ctttgaagag cgtgccggtg
     1621 ccgccgccgc tgtagggagc ggcccacgcg gtgatatatt cgatcgtctg gtcggcgagc
     1681 gattcgtaga gatcgccttt ctgcgggaaa cgcatgcccg ccggtatcag accccgctcg
     1741 tactccttcg cccacgccat ctcggcgcgg ccgatggatg cgcgctttcc cgctttcgcc
     1801 tcgtccacaa ggtcggcata ggtgagtttc caatcaggcg gcatcggcgg tacaggggtc
     1861 ggcttcagga tgcgcggctt accgataagc cggcggagga tgtcgcccag atcaatgtcc
     1921 cagcccatgg cgcatcagga tcgcgtgttc gagcgttttg agtatctcgg ccatatattc
     1981 ttcgacggcg cgaacgcttc ccggcagggc atagatgatc gtgtcgccca tcaccgccgc
     2041 gacgctgcgc gatagaagtg ctgacggttt atcggtgccg tacttcacgc ggatgtgttc
     2101 catgatgccc ggtatctccc tgtcggcgat ctcgagtatc acctcggggg tgatgtctcg
     2161 tggcccgatg ccggtgccgc cggtcgtgac gaccacatcg gtcgcctcgt cgcggtcgcg
     2221 ttcgagtatc ttcgcgagca tctccggctc atcgggaatg accgttcggt ctatcgcgag
     2281 atgccagcgc gtaccggtga aatgggcggc gatcatctcg gcgatgcgag gaccggagcg
     2341 gtcttcgtag tctcccgtcg cggcgcgatc ggagagggtc acgacatgga cattgagcgt
     2401 ccgttcgatg agttcggcct tctcgcccac cttcacgatg cccggcgtga tgacgcgggc
     2461 gaaaagtccc tgtttgggca tgagacattc accgacccgc tgaaagaccg agcatccggc
     2521 gccgtggcat tccttgccta tctgcgttat ctccaattcc accggtcccg cgatgaggcg
     2581 gtcgagcggt gcggcgcgca ggaggtcgac gcgcgcacgg acgtgtacgc catgtgcacg
     2641 gtgctgtacg agttcctcac gctcaactac tacctcggga acgtgggcga ggagatgagc
     2701 gacctggtgg gcgcgatcct gaaccgcaag ccccccgacg ccgagtcctg gtcagacccg
     2761 ctcaacgggc gcgtcccgcg cgcgctctcg cgcatcctcc gcaaggggct ctcgaagaag
     2821 cgggtcgacc gtttccagaa cgcgggcgag ctggaggagg tgctccagga ctggatggag
     2881 ggaaacgctc cggtggtctg ccccgggacc gcgatgcagc gcgggctgtc gcgctacagc
     2941 cgctggatcg accggcaccc ggtgttcgtg ccggccttca cgatcatcct ggggatc
//
LOCUS       DAOQUE010000366         2995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_38461_length_2995_cov_3.592177, whole genome shotgun sequence.
ACCESSION   DAOQUE010000366 DAOQUE010000000
VERSION     DAOQUE010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38461_length_2995_cov_3.592177"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="PKH10_10415"
     CDS             <1..1431
                     /locus_tag="PKH10_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HNT28577.1"
                     /translation="VTYRSVDHAALLTPIAPLDHGTQYVVTVTTNIADLAGNPLEEEQ
                     VFSFVTTLCGNGITDFGEQCDDGNTNDDDACTNACKPAVCGDGVRRDSYFYEEESLRL
                     DLDEGAGTTLYDTSGNENHGVINGASWVTAGYSGNALYFDGENDYIQWNYADTPLNDF
                     TMAAWVRPIAPHEIDAESTSGTSGSSGQKYVFVPEHKAENAGAGISIGTNGISVYEHG
                     EMYMPPLAVYAGTISSTEWTYLVVTYTAKQPRIYVNGVLVRTGLTSPRPIVYPPTSSG
                     GGDYGFFNGRIDRVRIYDRALTAQEIASGALDGFEQCDDGNTDDSDGCSNDCRLPHCG
                     DSNLDLYEECDDGNTMSGDGCDVHCKAHPYNNAVFISQSVPSSMQAGLTYGVSVTFQN
                     TGNTTWSTSDKYSLGSQNPQDNSTWGFTRVGLGTEVIQPEQSKTFNFDVTAPGTPGTY
                     NFQWKMVRDTYEWFGTQSANIPIVVN"
     gene            complement(1457..2863)
                     /locus_tag="PKH10_10420"
     CDS             complement(1457..2863)
                     /locus_tag="PKH10_10420"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT28578.1"
                     /translation="MNERSPHAPFRVLVVDDEQLFAQAIGREVERLGVACDLAYTAAD
                     ALRMAELTTYHLFLLDHKLPDDDGIRIIPLILSRQPTAALVMMTAFETIPNAVAAIRQ
                     GAEDYLVKQTNLKPIVEKVKEVRSRVQARESRKGWDDHERSRLIGDSPAILRAIAEMK
                     KVAQSPQMTVLITGESGVGKEVAAQYLHRLSCGENAPFMPVDCVTLPAALIENILFGH
                     EKGALAGADRAADGVFAKAGTGTVFLDGVGDLGTELQGRLLRVLETRTFHRTGSSQEE
                     PLRARIVASTHRDLSELVAEGKFRFDLSQRLAVFPIAIPPLRERREDVPSLTRHFIEF
                     TAQKLGHPSPLPLSAEIDEILRNYDYPGNVRELKNIIERAMVLADGSPLGAKHFPERI
                     QRIPVSSFTIGSRKNEVPVDFIPGVDSLETLEKKMIVNALRQTKGARSAAAELLGISR
                     FQLLRRIEKYGLGDTAEE"
     gene            complement(2860..>2995)
                     /locus_tag="PKH10_10425"
     CDS             complement(2860..>2995)
                     /locus_tag="PKH10_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT28579.1"
                     /translation="KNGTGLGLTVCKKIVEIHGGAIRLDNRPEGGCRATVSLPRRSSP
                     "
BASE COUNT          604 a          813 c          938 g          640 t
ORIGIN      
        1 gtaacttatc gctcggtcga ccacgcggca ttattgacgc ccatagcgcc cctcgatcat
       61 ggaacccaat atgtggtaac ggtaacgacg aacatcgctg atcttgcggg caatccgctg
      121 gaagaagagc aagtttttag ttttgttaca accctctgtg gcaacggcat aacggatttc
      181 ggggaacaat gtgatgatgg caatacaaac gatgatgatg cctgcactaa tgcctgtaag
      241 ccggcggtct gcggtgacgg cgtaagaaga gattcctatt tctatgagga agagtctctg
      301 cgcttggatc ttgatgaggg ggccggtaca accctgtacg acacatcagg gaatgagaac
      361 catggcgtga tcaacggtgc atcttgggtg acggcggggt actcgggcaa tgccctttat
      421 tttgatgggg agaacgatta tatccagtgg aattacgccg acacgccgtt aaacgatttc
      481 accatggcgg catgggtcag gccgatagcg ccgcatgaga tcgatgccga atcgaccagc
      541 ggaacaagcg gaagcagtgg acagaagtat gtgttcgtcc ctgaacataa ggcggagaat
      601 gcaggcgcgg gtatatcgat cgggacgaat ggaatttcgg tctacgaaca tggtgaaatg
      661 tatatgcctc ctctcgcggt gtatgccggc acgatcagca gcactgaatg gacgtacctg
      721 gtcgtgacct ataccgcaaa acagccgcgc atttatgtga acggtgtttt ggtcaggacc
      781 gggctgacct ctccccgtcc cattgtgtat ccgcccacaa gttcgggggg aggcgactac
      841 ggattcttca acggtcgtat cgacagggtc cgcatctatg atcgagcatt gacggctcag
      901 gagatcgcga gcggcgccct tgacggcttc gaacagtgcg atgacggcaa cacggatgat
      961 agtgatggtt gctccaatga ctgtcgtctt ccccactgcg gtgatagtaa ccttgacctg
     1021 tacgaggagt gcgacgacgg gaacaccatg tcaggtgacg gttgtgatgt tcactgcaag
     1081 gcgcatccgt acaacaacgc cgtttttatt tcgcaaagtg taccgtcttc catgcaggcg
     1141 ggtcttacat acggggtctc cgtcactttc cagaacaccg gcaacacgac gtggagtact
     1201 tccgataaat atagtttggg ttcccagaat ccccaagata acagcacatg gggctttacc
     1261 cgggttggac tagggacgga ggtgatccag cccgaacaaa gtaagacatt taattttgat
     1321 gttaccgctc ccggaacccc cggtacctat aatttccaat ggaaaatggt cagggatact
     1381 tacgaatggt tcggtacgca atcggcaaat atccctatcg tggtcaacta gagatttttt
     1441 tcgttgagtg atccgctcac tcctcggcgg tatcgccgag tccgtatttc tcgatgcggc
     1501 gcagaagttg gaaccggctg atgccgagca gttcggcggc ggccgagcgc gcccccttgg
     1561 tctgtcggag cgcgttgact atcatcttct tctcgagcgt ctcgagcgaa tcgacgccgg
     1621 ggatgaaatc caccgggacc tcgttcttgc gcgacccgat agtgaacgac gagacgggga
     1681 tgcgctgtat ccgttcggga aaatgctttg cgccgagcgg cgaaccgtcg gcgagcacca
     1741 tggctcgctc gatgatgttc ttgagttccc gcacattgcc ggggtagtca tagttccgca
     1801 gtatctcatc gatctcggcc gaaagcggta ggggggaggg gtggccgagt ttctgcgcgg
     1861 tgaattcgat gaagtgacgt gtgagcgacg gtacgtcctc gcgccgttcc ctgagcggcg
     1921 ggatggcgat cgggaacacc gcgaggcgtt gggaaaggtc gaagcggaac ttgccttcgg
     1981 cgacgagttc cgacagatcg cgatgggtcg aggcgacgat gcgggcgcgt agcggctcct
     2041 cctgcgagga tccggtccgg tggaatgtac gtgtctcgag cacgcggagc aggcgtccct
     2101 gcagttcggt gcccagatcg cctaccccgt cgaggaacac ggtgccggtc cccgccttgg
     2161 cgaagacgcc gtcggccgcg cggtcggccc cggcgagggc tcccttctcg tgaccgaaga
     2221 ggatgttctc gatgagcgcg gcgggtagcg tgacacagtc gaccggcatg aacggcgcgt
     2281 tctcgccgca ggagagacgg tgcaggtact gtgccgcgac ctccttgccg acgcccgatt
     2341 caccggtaat gagtacggtc atctgcgggc tctgtgcgac ctttttcatc tcggcgatcg
     2401 cacggagtat cgcgggcgag tcgccgatca gtctggagcg ctcgtgatcg tcccaccctt
     2461 tccggctttc ccgggcctgc acgcggcttc ggacctcctt gaccttttcc acgatcggct
     2521 tcaggttggt ctgcttcacc agataatcct cggctccctg ccgtatcgcc gcgacggcgt
     2581 tcggtatcgt ctcgaacgcg gtcatcatga cgagcgccgc tgtgggctgg cgcgacagga
     2641 tgagagggat gatgcgaatg ccgtcatcgt cgggcagttt gtggtcgagc aggaacaggt
     2701 ggtaggtggt tagttcagcc atgcggagcg cgtcggcggc ggtgtaggcg agatcacacg
     2761 cgacgccgag ccgctcgacc tcacgaccga tggcctgtgc gaagagttgc tcgtcgtcca
     2821 ccacaaggac gcgaaagggg gcatgagggg atcgttcgtt catggcgatg acctccgggg
     2881 aagcgatacg gtggcgcggc acccgccctc gggccgatta tcgagccgga tggcgccgcc
     2941 gtggatctcg actatcttct tgcataccgt gagcccgagt cccgtgccgt tcttc
//
LOCUS       DAOQUE010000367         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_39041_length_2964_cov_3.539704, whole genome shotgun sequence.
ACCESSION   DAOQUE010000367 DAOQUE010000000
VERSION     DAOQUE010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39041_length_2964_cov_3.539704"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PKH10_10430"
     CDS             complement(<1..320)
                     /locus_tag="PKH10_10430"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNT28580.1"
                     /translation="MTTTAHTEEKILFTQGNIACAEGALYAGCNFFGGYPITPSTEVA
                     EHMAEELPKRNGAFIQMEDEIASLGAVIGAALAGAKAMTATSGPGFSLMQELIGYACI
                     AEVP"
     gene            complement(330..578)
                     /locus_tag="PKH10_10435"
     CDS             complement(330..578)
                     /locus_tag="PKH10_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT28581.1"
                     /translation="MAAKKFPEEVFENPKCTLRIIPELCKGCAICIEFCPVKALSLDR
                     KRLIVTASAMEKCIACGMCELRCPDFAIVIDKKEKEKQ"
     gene            complement(603..1115)
                     /locus_tag="PKH10_10440"
     CDS             complement(603..1115)
                     /locus_tag="PKH10_10440"
                     /inference="COORDINATES: protein motif:HMM:NF017494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HNT28582.1"
                     /translation="MRKIPVADLDALRSVKMGEFVALEGVLLAMRDRTLARLAELKKN
                     GEPLPVPLAGRVVFFAGPTPGLADGRGAVGPTTSRRMAAYLPFLCDTGPVAIIGKGLF
                     DEKDAERLRSCNILYLQAIGGAGAYYGARAGGVGAVAFEEYGPEALFELTVHDFVVMM
                     TLDGAGAAWR"
     gene            complement(1112..1930)
                     /locus_tag="PKH10_10445"
     CDS             complement(1112..1930)
                     /locus_tag="PKH10_10445"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNT28583.1"
                     /translation="MRIVDGETIISALARLIGELVVDPPSLRDTAFCAPDLTGAEREA
                     AAILDENLRIARAERRPLCQDCGMVQVWVKKGKFASFSGASTLRPLIDEAVRRAYRDG
                     GLRCSMVKDPFDRKNSGDNTPASIHIEELDGADLEFTVLLKGGGSENVSRVAMLPPSA
                     GRAGVADIVLSALREAGGSGCPPYHVAIGVGGSFDTVATLAKQALLFDRGDDPELRDL
                     ILRRVPELGYGIAGFPGVRPVTAVRVATAPTHIAMLPVAVLLNCHAFRRGGVSL"
     gene            2079..>2964
                     /locus_tag="PKH10_10450"
     CDS             2079..>2964
                     /locus_tag="PKH10_10450"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNT28584.1"
                     /translation="MKSSQFFEKIDNLLKTHQKVEFNVSREELIFHAVTRGKALITVT
                     GTLATWTPVESTGRSPKDTLIVKRPESETKIDWTSPACNPCTPETFEMLLDDAIEFLS
                     KKQSFYVTDRALGADPAYALPCRTITDRSLVALFTDNMFRPVPKDIGKSMFADKGFTI
                     LAIPYNKLDPAKYVGRLRKLPSGKTTDMAIVMDFDNRIGIVIGSAYLGTVKKMMFTVM
                     NYYLPDANILPLHCSANEGPKGDCALLLGLSGTGKTTLSADPERALLGDDEHGWDDHG
                     IANFENGCYAKLINLNREK"
BASE COUNT          579 a         1005 c          878 g          502 t
ORIGIN      
        1 cacggcacct cggcgatgca ggcgtacccg atgagttcct gcatgagcga gaacccgggg
       61 ccggaggtcg cggtcatcgc cttcgcgccg gcaagcgccg cgccgatgac ggcgccgagc
      121 gacgcgatct cgtcctccat ctggatgaag gcgccgttcc gcttgggcag ttcctcggcc
      181 atgtgctccg ccacctcggt cgagggggtt atcggatagc cgccgaaaaa gttgcacccg
      241 gcatacagcg ccccttcggc gcaggcgatg ttgccctgcg tgaacaggat cttttcttcg
      301 gtatgggcgg tggtcgtcat ggggtcacct cactgcttct ctttttcttt cttgtcgatg
      361 acgatggcga agtcgggaca gcgcagttcg cacatgccgc aggcgatgca tttctccatc
      421 gcgcttgcgg tcacgatgag ccgcttcctg tcgagtgaaa gcgccttcac ggggcagaac
      481 tcgatgcaga tggcgcaccc tttgcacagt tcagggatga tgcgcagggt gcacttggga
      541 ttctcgaaaa cctcttccgg gaacttcttg gcagccatcg gtatcctcac tggttgataa
      601 ggtcatcgcc acgcggcgcc cgcgccgtcg agggtcatca ttacaacaaa atcgtgcact
      661 gtcaactcga acagcgcttc gggaccatat tcttcgaacg cgaccgcccc cacaccgccg
      721 gcccgcgccc cgtaataggc gccggcgcca cctatcgcct gcaaatacaa tatgttgcaa
      781 gaacgcaagc gttccgcatc cttttcatcg aacagacctt tgccgatgat cgccaccggt
      841 cccgtgtcgc agaggaaggg caggtaggcg gccatccgcc ggctcgtggt cggcccgacc
      901 gcgccgcgcc cgtcggcaag acccggggtc ggaccggcga agaagacgac gcgaccggcg
      961 agcggcaccg gcaacggttc gccgttcttc ttcaactcgg ccagtcgcgc cagcgtccgg
     1021 tcgcgcatcg cgagcaggac tccctccaac gccacgaact cgcccatctt caccgagcgg
     1081 agcgcatcga gatcggcgac cggtatcttt ctcacagcga caccccgccg cgccggaagg
     1141 catgacagtt gagcaggacc gcgaccggca gcatcgcgat atgggtcggc gccgtggcga
     1201 cacggaccgc cgtcacgggg cgaacgcccg ggaacccggc gataccgtag ccgagttccg
     1261 gcacccgccg caggatgagg tcgcgcagtt ccggatcgtc gccgcggtcg aacagaagcg
     1321 cctgcttcgc gagcgtcgca acggtatcga aactcccacc gacaccgatc gccacatggt
     1381 agggcggaca gccgctcccg ccggcctccc gcagggccga cagcacgata tcggcgaccc
     1441 ctgcgcggcc cgccgagggg ggaagcatcg cgacccggct cacattctcg gacccgcccc
     1501 ctttgagcag aacggtgaac tctagatcgg cgccgtccaa ctcctcgatg tgaatgctcg
     1561 caggggtgtt gtcgccgctg ttcttccggt cgaacggatc tttcaccatc gaacagcgca
     1621 ggccaccgtc gcggtaggcg cgccgcaccg cctcgtcaat gagcggccgg agcgtgctcg
     1681 cgccggagaa cgaggcgaac ttcccctttt tcacccacac ctgtaccatg ccgcagtcct
     1741 gacagagggg gcgccgctcg gcgcgggcga tgcggaggtt ctcgtcgagg atggcggccg
     1801 cctcgcgctc agcccccgta agatccggcg cacagaacgc agtgtcccgc aaggaggggg
     1861 gatcgacgac cagttcgccg ataagccgcg ccaaggcgga gatgatggtc tcgccgtcca
     1921 cgatcctcat gcccgcagta ttggcaccgc gcggcggcaa agtcaagaag aaccgacctt
     1981 cggggagcca tcccccaaaa ttattgtcaa agcccgttca ttcggctatg atggcgcggt
     2041 cgcttggctt attattttct tctaagggga gcccgttcat gaagtcgtcg caattcttcg
     2101 agaagatcga caatctgctc aagacccacc agaaagtgga attcaatgtg tcgcgcgagg
     2161 aactcatatt ccacgccgtg acacgcggca aggcgctcat cacggtgacc ggcacgctcg
     2221 ccacctggac cccggtcgaa tcgaccggcc gcagtcccaa ggacacgctc atcgtcaagc
     2281 ggcccgaaag cgagacgaag atagattgga cgagccccgc gtgcaacccc tgcacccccg
     2341 agaccttcga gatgctcctc gacgacgcga tcgagttcct ctcaaagaag cagtcgttct
     2401 atgtgaccga ccgagcactc ggcgccgacc cggcctacgc cctcccctgc cgcaccatca
     2461 ccgaccggtc gctcgtggcg ctcttcaccg acaacatgtt ccgccccgtg cccaaagata
     2521 tcggcaagag catgttcgcc gacaagggct tcaccatcct cgcgatcccc tacaacaaac
     2581 tcgatccggc caagtatgtc ggtcgcctgc gcaaactgcc cagcggcaaa acgaccgaca
     2641 tggcgatcgt gatggatttc gacaaccgta tcggcatcgt catcgggtcg gcctatctcg
     2701 ggacggtcaa gaagatgatg ttcaccgtga tgaactacta cctgcccgac gcgaacatac
     2761 ttccgctcca ctgctcggcc aacgaagggc cgaagggtga ctgcgcgctc ctgctcggcc
     2821 tgtcgggcac cggcaagacg acgctgtcgg ccgacccgga acgggccctg ctcggcgacg
     2881 acgagcacgg gtgggacgat cacggcatcg cgaatttcga gaacggctgt tacgccaaac
     2941 tcattaacct caaccgggag aaag
//
LOCUS       DAOQUE010000368         2959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_39138_length_2959_cov_4.142218, whole genome shotgun sequence.
ACCESSION   DAOQUE010000368 DAOQUE010000000
VERSION     DAOQUE010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39138_length_2959_cov_4.142218"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PKH10_10455"
     CDS             complement(<1..356)
                     /locus_tag="PKH10_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNT28585.1"
                     /translation="MCGIAGMFWVPGAERYVADLLFAIQHRGQESCGISVRRDDDTIA
                     TQKQMGLVKHVFTPETLDRFTGSSAIGHVRYPTAGSSDILNSQPHAIPLPDGSTLALC
                     ANGDIVNYAEWRTRLE"
     gene            517..2640
                     /locus_tag="PKH10_10460"
     CDS             517..2640
                     /locus_tag="PKH10_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28586.1"
                     /translation="MMSFSRIVILFFVAVVIVSCEPGTRRIFPPTTDQGSGNADTDEV
                     FLDSDDLLPDEPGSDTGETETDPLVNDDGQLVNDDGELVNDDGQLINDDGQVVNDDGQ
                     VVNDDGEVVTDDIVNDEVVTDEVATDDVTTDDILTDGIATDDGTVVTDDGTVVADDGT
                     VITDDAVVPDVDVDTCECSTGACCDGCHFRTADSNYVCRSAAHPVCDVVETCPGNSAA
                     CPPDGFAPDTTPCDDGVFCNGDDTCNGNSAACDVHDGFFCGNFVCSESQGKCCDAGWA
                     GADCEICVRFVKTTPAPGTPDGLSWNTAFNDLQAAIDSANGSSAATCEVWVAAGTYYA
                     YKNATGNYIQLRGKVPFYGGFAGTEALRESRDWTVNATIIDGRQSSGGSDRVSHILYG
                     AAGALMDGFTVQEGRAVTGSGSGNIVEGGAMYLNNASMTVRNCIFRNNSAVVQTGDDT
                     ARGGAIAIIGGGSSLFENCLFFKNTVTAGNYDVAFGGAIYATGGGTTTVRNCVFSENT
                     ITGGTSTEAGGGALNGYGTGTVLEVVNSLFYSNTVTAPTANGGAIRANDASAIQITNS
                     TFSKNSAEDSGGAVANMGGTGTTIVNSIFWADAAATVQNNTDELWDEGGGGTIAATYS
                     DIQMTNDSNLSGTGNIHADPLFVDAPNNDFHLIADPVGMQISPCIDFASDASAPTTDL
                     EGKTRFDVPMYGTGTADMGCYEFVY"
     gene            complement(2637..>2959)
                     /locus_tag="PKH10_10465"
     CDS             complement(2637..>2959)
                     /locus_tag="PKH10_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNT28587.1"
                     /translation="MASRPVGVAVASEWVAALNRFARLPDTVIYIEVSIDTALSRLTR
                     FRQNAEIFETRGFLEKLRANYERVLTEYADQVNIVRLDGEVAMGDIAADIEIRLSGIF
                     PKKS"
BASE COUNT          582 a          879 c          935 g          563 t
ORIGIN      
        1 tgttcaagac gggtgcgcca ttcggcgtag ttgacgatgt cgccgttcgc acagagggcg
       61 agcgtcgaac catcgggcaa gggtatggcg tgcggctggc tgttgagtat gtcgcttgac
      121 ccggcggtcg ggtaacggac atggccgatg gcggaagacc cggtgaaacg atcgagcgtc
      181 tcgggagtga agacatgctt gacgagtccc atctgtttct gggtcgcgat ggtgtcatca
      241 tcgcggcgta ccgatatgcc gcacgactcc tgaccgcggt gctgtatcgc gaagaggagg
      301 tcggcgacat agcgctccgc ccccggcacc cagaacatcc cggcgatacc gcacatcgaa
      361 cttcacctca taatgatcgg tccgcgccag ttatagccac cgtcgcgcca aaaagcaagg
      421 aatcgaagcg cggccccttt tcgcttgact ttacacggtg ccggggatac aatgcctcct
      481 gtttttattt gatttttttc cgcgagggag gagaaaatga tgtctttcag ccgcatcgtg
      541 atcctgtttt tcgtggcggt ggtcatcgtc tcctgcgagc cggggacccg ccggatattc
      601 ccgccgacca ccgatcaggg gagcggtaat gccgacacgg atgaggtctt cctcgacagc
      661 gacgatctgc ttcccgacga gccggggagc gataccgggg agacggagac cgatccgctc
      721 gtgaacgacg acggccagtt ggtcaacgac gacggcgaac tcgtgaacga cgacggacag
      781 ttgataaatg atgacggcca agtggtgaac gacgacggtc aggtggtgaa tgacgatggc
      841 gaggttgtga ccgacgatat cgtgaacgat gaggtggtca ccgatgaggt ggctacagac
      901 gatgtgacta ccgacgatat cctcactgac gggatcgcga ccgatgatgg taccgtggtc
      961 accgatgatg gtaccgtggt cgccgacgac ggtaccgtga tcactgatga tgcggtcgtg
     1021 cccgatgtcg atgtcgatac ctgcgaatgt tcgaccggcg cctgttgcga tggctgtcat
     1081 ttccgtacgg cggattcgaa ttatgtctgt cgttccgctg cccacccggt gtgcgatgtt
     1141 gtcgagacct gccccggcaa cagtgccgcc tgtccccccg acgggttcgc tcccgatacc
     1201 acgccgtgcg acgatggcgt gttctgcaac ggggacgata cctgcaacgg caacagcgcc
     1261 gcctgcgatg tccatgacgg gtttttctgc ggtaacttcg tctgtagcga aagccaagga
     1321 aaatgctgtg acgccggctg ggcgggcgcc gattgcgaga tctgcgtccg gttcgtgaag
     1381 accacgcctg cgcccggtac gcctgacggc ctttcgtgga acaccgcctt caacgatctg
     1441 caggcggcga tagattcggc caacggctcc agcgccgcga cctgcgaggt ctgggtcgcc
     1501 gcggggacct attacgccta caagaacgcg accggtaact acatccagtt gcgtggcaag
     1561 gtgccgttct acggtgggtt cgcggggacc gaggcgctcc gcgaaagccg tgactggacg
     1621 gtgaacgcca ccatcatcga cgggcggcag tcatcggggg gcagcgaccg cgtttcccat
     1681 atactgtacg gcgcggccgg cgcgttgatg gatggcttca cggtgcagga gggacgcgcg
     1741 gtgaccggca gcggttccgg caatatcgtc gaaggcggcg cgatgtatct caacaacgct
     1801 tcgatgaccg tccgcaactg tatcttcagg aataactccg ccgtcgtgca gaccggtgac
     1861 gacaccgccc gtggcggcgc gatcgcgatc atcggcggcg ggagcagcct gttcgagaat
     1921 tgcctctttt tcaagaacac ggtcacggcg ggcaactacg atgtcgcgtt cggcggcgcg
     1981 atctacgcga ccggcggcgg cacgaccacc gtgcgcaact gcgttttctc cgagaacacg
     2041 atcaccggcg gcacttccac cgaggccggc ggcggtgcgc tcaacggcta tgggaccggt
     2101 acggtactgg aggtggtcaa ctcgctcttc tacagcaaca cggtcaccgc gccgaccgcg
     2161 aacggcggcg cgatacgggc gaacgatgcc tcggcgatac agatcaccaa cagcaccttc
     2221 tcaaagaaca gtgcggagga tagcggcggc gcggtagcca acatgggagg caccggcacg
     2281 acgatcgtca actccatctt ctgggccgac gcggcggcga cggtgcagaa caacactgac
     2341 gaactctggg acgagggcgg cggcggcacg atcgccgcga cctacagcga catccagatg
     2401 accaacgaca gcaacttatc cggcaccggc aacatacacg ccgatccact gttcgtcgat
     2461 gcgccgaaca acgatttcca tctgatcgcc gatccggtcg ggatgcagat atccccctgc
     2521 atcgacttcg cgagcgacgc atcggctccg accaccgatc tcgaaggcaa gacccgtttc
     2581 gatgtgccga tgtacggtac cggcaccgcc gatatgggct gttacgagtt cgtctattaa
     2641 ctctttttcg ggaagatgcc ggatagtctt atttctatgt cggcggcgat atcacccatc
     2701 gccacctcgc cgtcgagccg cacgatgttc acttggtcgg catactccgt gaggacccgt
     2761 tcatagttcg ccctgagttt ttcaaggaag ccgcgcgttt cgaatatctc ggcgttctgc
     2821 cggaaccgcg taagtctcga aagtgcggtg tcgatgctca cttcgatata gatgacggtg
     2881 tcgggaagcc gtgcgaaccg gttgagggcg gcgacccatt cggaggccac ggccactccc
     2941 acgggccggg aggccattc
//
LOCUS       DAOQUE010000369         2948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_39358_length_2948_cov_4.217767, whole genome shotgun sequence.
ACCESSION   DAOQUE010000369 DAOQUE010000000
VERSION     DAOQUE010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39358_length_2948_cov_4.217767"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="PKH10_10470"
     CDS             <1..1127
                     /locus_tag="PKH10_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNT28588.1"
                     /translation="VFWMGHGLPTMSVRSIWHHDNGDGIPSSYEISSEVFVAAYTTPQ
                     LPGDMASFVYMGSCLNGDVDEPYNLAYTMLRDTAVGVAASSQVSYGVIFSEYDPSQAQ
                     DVFAYGTVFSQAVLEGRSPALELAEYKKYWSSRSVLKTMKMEINYFGDPTLSSGVRAC
                     VSDTDCDDGLFCNGVERCHEGYCGLREAPIVCPAPSAVCRTAVCDEATKGCLTVSSMD
                     GVPCGGEENKCFDRSVCRAGACEYEGATDCSLFDGPCARGECDPATGACRAVPENEGE
                     SCDDGYHCTTGDTCETGECVGDAVVCSETADCRYQVCDESAEGACIALQDLGQNGKAC
                     ETTDGLDGYCSYGECVAAPEDKDKEGTTGGGGCSMTTIGR"
     gene            complement(1105..2727)
                     /locus_tag="PKH10_10475"
     CDS             complement(1105..2727)
                     /locus_tag="PKH10_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNT28589.1"
                     /translation="MAIKIGEYFVQFGKITRQQLTQALELQKKQPGFLGEILVGEKMI
                     TENDLLSYLAGIFNLQYITSEKLEKMTIQPNVAQIVPEKIAESKLLFPLKYEFKGGKL
                     SLLTNAPQNKAVFDELKLILDNVHDITPVIAYSKAIKAIVAKQYRGDLIAFDRYFPKS
                     VDLKEDFLPVGEAVLDKDMLKQDAPRKDFSGTRKTSLTQIINRGEILQADGDTTITNR
                     VNRLQNGVTVLGHEEFIEAVKVFTSIIDSIREGAFKNHTQRVVSLCIKLGKAYQLNEV
                     EMDELVVAAYLHDAGKKKHVTSFDIKDKACADTIIKYTSAGVRLFAGCNLPKNTKNIL
                     SNMYEVANGEGYPQGLKTKDIPIGAQILLLADTFDYLSQVSAIPAPTAFFRIKATGHF
                     DEKLLNTLKEEMQINTTAAGRVKLAGVVVTENRELLDQLAQEFGNHNVQVFTAPQIEI
                     AALILKKQGETGINFVLADINVRNSQYTPFKLLSALRAKGIFRKTQFFMFATGGITAQ
                     DNEQAAQLQASGLFPNLQVEKAVLDVIARSSS"
BASE COUNT          506 a          828 c          969 g          645 t
ORIGIN      
        1 tcgtgttctg gatggggcac ggtctgccga cgatgtcggt caggtcgatc tggcaccacg
       61 ataacggtga cgggatcccc tcctcctacg agatatcgtc cgaggtgttc gtcgccgcct
      121 acacgacccc gcagttgccg ggcgatatgg cgtccttcgt ctatatgggc agttgtctga
      181 acggcgatgt ggacgagccc tacaacctcg cctacacgat gctccgcgac acggcggtcg
      241 gcgtcgcggc ctcttcgcag gtcagttacg gcgtcatctt ctccgaatac gatccgtcgc
      301 aggctcagga tgtgttcgcc tacggcacgg tattctcgca ggcggtgttg gagggtcgtt
      361 cgccggcgct ggaactcgcc gaatacaaaa aatactggtc cagccgatcg gtgctgaaga
      421 ccatgaagat ggagataaac tatttcggcg acccgacgct ttcaagcggc gtgcgggcct
      481 gcgtttccga tacggactgc gacgacggtc tcttctgcaa cggtgtcgag cggtgtcacg
      541 aggggtactg cggcctgcgc gaggcgccga tagtatgtcc ggcgccgtcg gccgtgtgcc
      601 gcaccgccgt ctgcgatgag gcgaccaagg ggtgtctcac ggtttcgagc atggatggcg
      661 ttccctgcgg cggcgaggag aacaagtgct tcgaccgttc ggtgtgtcgc gccggtgcct
      721 gcgagtacga gggggcgacc gactgttcgc tgttcgacgg tccctgtgcc cgcggcgagt
      781 gcgatccggc gaccggcgcc tgccgcgccg tgccggaaaa cgaaggcgag tcctgcgacg
      841 acggctacca ctgcacgacc ggtgacactt gtgagacggg ggaatgcgtc ggcgacgcgg
      901 tggtgtgcag cgagaccgcc gattgccggt atcaggtgtg cgacgaatcg gcggaaggcg
      961 cctgcatcgc cttgcaggac ctcggccaga acgggaaggc ctgcgaaacg accgacgggc
     1021 ttgacggcta ctgttcctac ggcgaatgcg tcgcggcgcc ggaagacaag gataaggaag
     1081 ggacgacggg cgggggcggg tgctctatga cgacgatcgg gcgatgacat cgagcaccgc
     1141 cttttcaacc tgaagattcg ggaacaggcc tgatgcctgc agttgcgccg cctgctcgtt
     1201 atcctgcgcc gtgatgccgc cggtagcgaa catgaagaat tgcgtcttac ggaatatccc
     1261 cttggcccgg agcgccgaaa gcagtttgaa cggggtgtac tgcgaattgc gcacattgat
     1321 gtcggcgagc acgaagttga tgcccgtctc cccctgtttt ttgaggatga gggccgctat
     1381 ctctatctgc ggggcggtga agacctgcac attgtggttg ccgaactcct gcgccagttg
     1441 atcgagcagt tcgcggttct cggtcacgac gacgccggcc agtttcacgc ggcctgccgc
     1501 cgtcgtgttt atctgcatct cctccttgag cgtgttcagg agtttctcgt cgaagtgccc
     1561 cgtcgccttt atccggaaaa aggcggtcgg cgccgggatg gccgatacct gcgagagata
     1621 gtcgaaggtg tcggcgagca ggagtatctg ggcgccgatc gggatgtcct tcgtcttgag
     1681 cccctgcggg tagccctcgc cgttcgcgac ctcgtacatg ttcgacagga tgttcttggt
     1741 gttcttcgga aggttgcacc cggcgaacag ccggacgccg gccgaggtgt atttgatgat
     1801 ggtgtcggcg cacgccttgt ccttgatgtc gaacgaggtg acatgtttct tcttcccggc
     1861 gtcgtgcagg taggcggcga cgacgagttc gtccatctcg acctcgttca actggtaggc
     1921 tttgcccagt ttgatgcaga gcgagacgac ccgctgggtg tggttcttga acgccccctc
     1981 gcgtatggag tcgatgatgg aggtgaagac cttgaccgcc tcgatgaact cctcgtgtcc
     2041 gagcacggtg acgccgttct gaaggcggtt gacgcggttg gtgatggtcg tgtcgccgtc
     2101 ggcctgcagt atctcgccgc ggttgatgat ctgggtcagc gaggtcttgc gcgtccccga
     2161 aaagtccttg cgcggggcgt cctgtttgag catgtcctta tcgagcaccg cctcgccgac
     2221 cggcaggaaa tcctccttga gatcgacgct cttggggaaa tagcggtcga acgcgatgag
     2281 gtcaccgcgg tactgtttgg cgactatcgc ctttatcgcc ttcgagtagg cgatgaccgg
     2341 cgtgatgtcg tgcacattgt cgaggatgag tttgagctcg tcgaagaccg ccttgttctg
     2401 cggggcattg gtgagcaggg aaagtttgcc gcccttgaat tcgtatttca gcgggaagag
     2461 tagtttcgat tcggcgatct tctcggggac gatctgggcg acattcggct ggatggtcat
     2521 cttctcgagc ttctcgctcg tgatgtattg gagattgaag atgccggcga ggtaggagag
     2581 caggtcgttc tccgttatca tcttttctcc gacgagtatc tcgccgagga acccgggctg
     2641 tttcttctgc agttcgagcg cctgcgtgag ttgctggcgg gtgatcttcc cgaactgtac
     2701 gaaatactcg ccgatcttga tggccacgat ggtccccgct cgctcaattg gtgtcaagac
     2761 gaacggattg tatttcggaa aaacgcaaaa tcaaatttat tataccgggc catcaaaagg
     2821 gggcgatgag cgcgcaggcg cacccgccgg acccgtcctc atcctccccg ggcaggatat
     2881 cctcgtcgga ggattctccg gcgtcgtcat gggcggccgg catctcgaca tcgacggcgt
     2941 cgccgctg
//
LOCUS       DAOQUE010000370         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_39424_length_2944_cov_3.879889, whole genome shotgun sequence.
ACCESSION   DAOQUE010000370 DAOQUE010000000
VERSION     DAOQUE010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39424_length_2944_cov_3.879889"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PKH10_10480"
     CDS             <1..740
                     /locus_tag="PKH10_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28590.1"
                     /translation="RLAVFDCGMAGTIDRYTREMFAEMLLAFAQKDYATLASVYAEIS
                     DAPVPVNKKQLARDIRRITEALPETLIEVDMSDLFSKVMKVLYRHRLRVPKNFTLLIR
                     GASMLEGLGRELVPDFNFIHESQELAAVVINAKYSPERVRENLYYLGMRFLDMGKTLP
                     LNLSEIIEKLENGNLKMNWEFFISGHDRLFISKMITRLVTGFLLTGLILSFNSFERTL
                     PFYLLYLPALTISIVMFILTFKKDRRR"
     gene            737..1807
                     /locus_tag="PKH10_10485"
     CDS             737..1807
                     /locus_tag="PKH10_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNT28591.1"
                     /translation="MRTSFSIREHLEQIESQTLSPHACLSTRSKGRERDEKPCPIRPV
                     FQHDRDRIVHSKAFRRLKDKTQVFLSPEGGHYRNRLTHTLEVSQIARTIARALSLNEQ
                     LTEAIAMGHDLGHTPFGHSGERALARLLPGGFRHEAQSLRMVETLSRRGAGLNLSFEV
                     RDGIAKHSKGKGKLLKKEGIPATLEGQVVRLADIIAYVNHDIDDAMNGGFMQEKDMPC
                     ADLLGRRVSVRINRMVFDLIQTSMDSLQAGDIRILMSDRMAAAIEEVRDFLYEEVYEA
                     PAIRHEFEKAERIVKALFDHFRDHYEHLSRFYEPLVAPEPERNIGDFIASLTDGYAIE
                     LYRHLFIPERLPYREFAHDDET"
     gene            1794..2792
                     /locus_tag="PKH10_10490"
     CDS             1794..2792
                     /locus_tag="PKH10_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28592.1"
                     /translation="MTRPRPLLLLALILSLALNIAAAVLLWRHYASSPTELTTECRLY
                     PEQECSIDFPASLREHRPLFSAYSIRGDRIVFLDFSLQGTIVESVMEKYGRELPNDLV
                     RNIQTLIYFFLDYQVRLGTNDRIALFYRPSDDRILYLRLRSRSYNAVFEAFLYQTAQR
                     EIYTTADGSTFPPCMKNGPFAGCPPVRLEKRHDVLVPLFLLPQNGEVRLPFDAWVISL
                     TDFSRNGGAVQVTFPDQGLVGFFDYLGSVNPQVKVGKRYKAGTLVGTGGFAKRDGADG
                     VRYYLERRDGTPVSPFFFHHTTTTVLSTEEKVNLTITQNFYRNWHRHGQKFENKHY"
BASE COUNT          586 a         1027 c          779 g          552 t
ORIGIN      
        1 accggctcgc tgtcttcgac tgcggcatgg cggggaccat cgaccgctat acgcgcgaga
       61 tgttcgccga gatgctgctg gccttcgcgc agaaggacta cgccacgctc gcctcggtct
      121 atgccgagat cagcgacgca ccggtcccgg tcaacaaaaa gcaactcgcc cgcgatatac
      181 gacgtatcac cgaggcgctc cccgagacgc tcatcgaggt ggatatgtcg gatctgttct
      241 ccaaggtcat gaaggtccta taccgccacc ggctccgcgt ccccaagaat ttcacgctcc
      301 tcatccgcgg cgcatcgatg ctcgaggggc tcgggcgcga actcgtcccc gacttcaact
      361 tcatccatga atcacaggaa ctcgccgcgg tggtcatcaa cgcgaaatac tcccccgaac
      421 gggtccgcga gaacctctac tatctcggca tgcgcttcct ggacatgggg aagacgctgc
      481 cgctcaatct gtcggagatc atcgagaaac tcgagaacgg caacctgaag atgaactggg
      541 agttcttcat cagcggtcac gatcgcctct tcatatcaaa gatgatcacg cgcctcgtga
      601 ccgggttcct tctcaccgga ctcattctct cattcaactc cttcgagcgg acactcccct
      661 tctacctgct ctaccttccg gcgctcacca tatcgatcgt gatgttcata ctcaccttca
      721 aaaaagaccg gcgccgatga ggacctcctt ctcgatacgc gaacatcttg aacagatcga
      781 gtcccagaca ctctcgccac acgcctgcct ctcgacccgc agcaagggac gcgagcgcga
      841 cgagaaaccg tgccccatcc gaccggtatt ccagcacgac cgcgaccgta tcgttcattc
      901 gaaggccttc cgacgcctca aggataagac acaggtgttc ctgtcaccgg agggcggtca
      961 ttaccgcaac cggctcaccc acaccctcga ggtctctcag atagcccgca ccatcgcccg
     1021 ggccctttcg ctcaacgaac aactcactga ggcgatcgcg atgggccacg acctgggtca
     1081 cacccccttc ggtcattcgg gggaacgggc gctcgcacgg ctcctgcccg gcgggttccg
     1141 ccacgaggcg cagagtctcc ggatggtcga gacgctcagc cgccgcggcg cgggactcaa
     1201 tctctcgttc gaggtacgcg acggtatcgc caaacattca aagggaaaag gtaaactcct
     1261 caagaaggag ggcatccccg ccaccctcga aggtcaggtt gtgcgactcg ccgacatcat
     1321 cgcctatgtc aaccacgaca tcgacgacgc gatgaacggc ggcttcatgc aggaaaagga
     1381 catgccctgc gccgacctgc tgggccgccg cgtatcggtg cgcatcaacc gcatggtgtt
     1441 cgacctgata cagacgagca tggactccct gcaggcgggg gacatccgta tcctcatgag
     1501 cgaccggatg gccgccgcca tcgaggaggt acgcgatttc ctgtacgagg aggtatacga
     1561 ggccccggcg atacggcacg aattcgaaaa agccgagcgc atcgtaaagg cgctgttcga
     1621 ccacttccgc gaccactacg agcatctttc ccgtttttac gaaccgctcg tcgcccccga
     1681 accggaacgc aacatcggcg atttcatcgc ctcgctgacc gacggctacg ccatcgaact
     1741 gtaccggcat ttgttcatcc ccgaacgact cccctaccgc gagttcgccc atgatgacga
     1801 gacctaggcc tctccttctg ctcgccctca tcctctcgct ggcactcaac atcgcggcgg
     1861 cggtcctgct gtggcgtcac tacgcctcct cgccgaccga gttgacgacc gagtgccggc
     1921 tctaccccga gcaggagtgt tcgatagatt tccccgcctc cctccgtgag caccgccccc
     1981 tattctccgc ttactcgata cggggagacc ggatcgtgtt cctcgacttc tccctgcagg
     2041 ggacgatcgt cgagtcggtc atggagaaat acggtcgcga actgccgaac gatctggtcc
     2101 gcaatatcca gacgctcatc tatttctttc tcgactatca ggtgcgactg ggcaccaacg
     2161 accgtatcgc actcttctac cgcccctccg acgaccggat actctacctc cgcctccgga
     2221 gccgatccta caacgcggtg ttcgaagctt tcctctacca gaccgcgcag cgcgagatat
     2281 atacgaccgc cgacggatcc acctttccgc cctgcatgaa gaacggcccc tttgccggat
     2341 gccctcccgt ccgcctcgag aaacggcacg atgtgctggt gccgctcttt cttcttcccc
     2401 agaacggcga ggtccgcctc cccttcgacg catgggtcat atcgctgacc gatttctccc
     2461 ggaatggtgg cgccgtgcag gtgaccttcc ccgatcaggg gcttgtgggc tttttcgact
     2521 atctcggctc cgtgaacccg caggtgaagg tcggcaaacg ctacaaggcg gggacgctcg
     2581 tggggaccgg cggtttcgcc aagcgcgacg gggccgacgg cgtccggtac tacctcgaac
     2641 gccgtgacgg cacgccggtc tcgcccttct tcttccacca caccaccacg acggtcctgt
     2701 cgaccgaaga gaaggtgaat ctcacgataa cgcagaattt ctaccgcaac tggcaccgtc
     2761 atggacagaa gttcgagaac aagcattact gagaggcgcc tatgacgaac ctgcggatac
     2821 tcgtcacctg cgacctgccg ggcgaccggc tccccgccct gcgacgcgac gaacggttct
     2881 ccgtgacggt cctcgacggc gacgcccgac gcgaccttcc cggcgccatc gccgccaccg
     2941 cgcc
//
LOCUS       DAOQUE010000371         2939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_39535_length_2939_cov_3.785021, whole genome shotgun sequence.
ACCESSION   DAOQUE010000371 DAOQUE010000000
VERSION     DAOQUE010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39535_length_2939_cov_3.785021"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1518
                     /locus_tag="PKH10_10495"
                     /pseudo
     CDS             1..1518
                     /locus_tag="PKH10_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
     gene            complement(1682..2128)
                     /locus_tag="PKH10_10500"
     CDS             complement(1682..2128)
                     /locus_tag="PKH10_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNT28593.1"
                     /translation="MKELTTRDILEHAAKIETESFAFYSLASEAMTDKELKELCQDLA
                     EQEKGHLNNVRALLKTLGPGHTIPAPAGTLERLIVHGKASPRSTPREILTIAHQRELN
                     TRQFYQTMLALTDLGPDMVKLFEELMRQEELHAESVKKRLERLTSS"
     gene            complement(2125..>2939)
                     /locus_tag="PKH10_10505"
     CDS             complement(2125..>2939)
                     /locus_tag="PKH10_10505"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT28594.1"
                     /translation="PYPDLDKDIAFVKELGVKITLNTRVGKDVTLDDLRKRFDSVFIS
                     TGLHLGRSTKVPGADHPKVFQAIDLLRRVTLGAEIYCPGEAIVIGGGNVAMDIARTIA
                     RLQTKKYGKVGVTLLCLETEDIMPADREEIVESREEGVILRPGYGPTRIETDGDRVTG
                     ARFVRCLSVFDENGRFAPRFDNNDEIAVSGAMVVESIGQGMDLSYLPDDLKDQIVFER
                     GRPKVDRHFRTSVPWLYAGGDIVQGPDVIHGIANGHAAAVGIDRYLREGGTK"
BASE COUNT          567 a          863 c          969 g          540 t
ORIGIN      
        1 cttgccttta cccttctcgg ccttgatgaa ctgcgggtgg agcagttggt cgaggccgag
       61 aagggtaaag gcaagatgat cgctcgcggt ctcccggcga gccccggcgc ggcgaccggt
      121 cgggtggtgt tcaacgccga ggatgccgag aaatggaccg agaagaagga aaaggtcatc
      181 ctcgtccgca tcgagaccag ccccgaggac atcggcggca tggcggtggc gcagggtatc
      241 ctcacggccc gcggcggcat gacgagccac gcggcggtcg tggcgcgcgg catgggcaaa
      301 tgctgtgtcg ccggctgcgg cgctctgcag atagattaca aggcgcggac cctcaaggcc
      361 gacaaggtga cggtgaaaga gggcgactgg atatcgctcg acggctcgac cggcgaggtc
      421 ttcctcggcc agataaagac cgaggcgccc aagttcgaag gcgagttcgg cgtgctgatg
      481 ggctgggccg atgcgcggcg cactctcaag gtccgcacca atgccgacac cccgcacgat
      541 tcgaccgtgg cgcgcaactt cggcgccgaa gggatcgggc tgacccgcac cgaacacatg
      601 ttcttcgaag gcgaccgcat cctcgcggtc cgcgagatga tactggccga tgacgagcag
      661 ggccgccgga aggcgctcgc gaaactcatc aagcaccagc gcgacgattt ctacggcatc
      721 ttcaaggcga tgcgcggcct gccggtcacc atccgcctgc tcgatccgcc gctccacgag
      781 ttcctgccgc aggaactccc ggcgcagaag gaaatggcgc agaccatgaa gatatcactg
      841 aaacaggtgc aggagaaggt cgaggaactt cacgagttca acccgatgct cggtcaccgt
      901 ggctgtcgtc tgggcatcac ctatcccgag atatcggaga tgcaggcgcg cgccatcttc
      961 gaggcggcgt gcgatctcgc gaaggaaaag gtgcaggtcc tgcccgaggt catgataccg
     1021 ctcgtcggcg acaagaagga atatctccat caggccgaca tcatcgtgaa gaccgccgaa
     1081 aaggtcttcg ccgagaagaa gataaagatc aactacatgg tcggcacgat gatagaggtc
     1141 ccgcgcggcg cggtgaccgc cgatgagatc gcgcagagcg ccgagttctt ctcgttcggc
     1201 accaacgacc tcacccagat gacgctcggc ttctcgcgag acgacgcggg tcgtttcatc
     1261 aaggtctata tcgacggcaa gatactcgaa aaggacccgt tccagtcgat agatcagacc
     1321 ggtgtcggtc aactggtcgc tatggcgtgc gagaaggggc gcaagacccg cccgaacctc
     1381 aaactcggcg tctgcggcga acacggcggc gaccccgatt cgatagactt cttccaccgc
     1441 gtcgggctca actatgtctc ctgttcaccc taccgcgtac cgatagcgcg cctcgcggcg
     1501 gcgcaggcgg ccatcaagca cgacaaggtg cggatggtca aagaggcggt caagaagggg
     1561 gccgaaacga tgggctcgcg cttcttcgcg accgtcaaga ccgccggcgc aaaggctgag
     1621 gtgctgggca aaaaggcgaa gatagtcgcc cggcgcaccg gaaagaatgt agagcggaaa
     1681 cttaagaaga agtaagccgt tccagccgtt tcttcacgct ttcggcgtga agttcctcct
     1741 gccgcatgag ttcttcgaag agtttcacca tatcggggcc gaggtcggtg agggccagca
     1801 tcgtctggta gaactgccgg gtgttgagtt ctcgctggtg ggcgatggtc agtatctcgc
     1861 gcggcgtcga acggggtgat gcctttccgt ggacgatgag ccgttcgagc gtgccggcag
     1921 gtgccggtat cgtgtggccg ggaccgagtg tcttgagaag cgcacggaca ttgttgagat
     1981 gtcctttctc ctgttcagcg aggtcttggc agagctcttt gagttccttg tcggtcattg
     2041 cctccgatgc gagcgaatag aaggcaaaac tctcggtctc tatctttgcg gcgtgttcga
     2101 ggatgtcgcg ggtggtcagt tccttcattt cgttcctcct tcacgcaggt agcggtcgat
     2161 gccgacggcg gcggcgtggc cgttggcgat gccgtgtatc acatcggggc cctgcacgat
     2221 gtcgccgccg gcgtagagcc acggcacgct cgtgcggaaa tgacggtcga ccttcgggcg
     2281 gccccgttcg aatacgatct ggtccttgag gtcgtcgggc aggtaggaga ggtccatccc
     2341 ctgaccgatc gattcgacca ccatcgcgcc gctgaccgcg atctcgtcgt tattgtcgaa
     2401 gcgcggggcg aagcggccgt tctcgtcgaa taccgacaga caccggacga agcgggcccc
     2461 cgtcacgcgg tcgccgtcgg tctcgatgcg ggtcggtccg tagccgggac gaaggatcac
     2521 cccctcttcg cgcgattcga cgatctcctc gcggtcggcc ggcatgatat cctcggtctc
     2581 aaggcagagg agcgtcacgc ccactttgcc gtatttcttg gtctgtaggc gggcgatcgt
     2641 ccgggcgatg tccatcgcga cattgccgcc gccgatgacg atggcctcac cggggcagta
     2701 gatctctgcg ccgagcgtca cgcggcgcaa caggtcgatc gcctgaaaga ccttgggatg
     2761 gtcggcgccg gggaccttgg tcgagcggcc gaggtggagg ccggtcgaga tgaagacgct
     2821 gtcgaatctc ttacgcagat cgtcgagggt gacatctttc ccgacgcggg tgttcagcgt
     2881 tatcttgacg ccgagttctt tgacaaaggc gatgtccttg tcgaggtcgg ggtagggga
//
LOCUS       DAOQUE010000372         2937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_39579_length_2937_cov_3.973629, whole genome shotgun sequence.
ACCESSION   DAOQUE010000372 DAOQUE010000000
VERSION     DAOQUE010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39579_length_2937_cov_3.973629"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKH10_10510"
     CDS             complement(<1..815)
                     /locus_tag="PKH10_10510"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT28595.1"
                     /translation="MTKHIMILVAMFLLAACGGDGTETPDKDTSATDTEQPDVDYDAG
                     TPGEMVDVPAGEFQMGCNSAVDLQCESDESPYHAVTLSAYKIGKYEVTVGEYHQCVTN
                     GVCNNSGEYHHYYTNTDSGDCNYDAEGKGNHPMNCVTWYGAKAYCEWIGGRLPTEAEW
                     EKAARGTDGRKYPWGNEDATCDYAVMDDDNAGGEGCGSGGTMPIGSKEVGKSPYGAYD
                     MAGNVWEWVNDWHGLDYYTSSPANNPTGPETGGFRVLRGGSWSNGYVNLLRAPA"
     gene            complement(944..1360)
                     /locus_tag="PKH10_10515"
     CDS             complement(944..1360)
                     /locus_tag="PKH10_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNT28596.1"
                     /translation="MKHHRKELSFTTTTRRAFINITPDVERAVAESGVKEGLVLVNAM
                     HITASVFINDDERGLHHDYEVWLEKLAPEKPHAQYRHNGAEDNADAHLKRQVMGREVV
                     VAITGGKLDFGPWEQIFYGEFDGMRRKRVLVKIIGD"
     gene            complement(1370..2365)
                     /locus_tag="PKH10_10520"
     CDS             complement(1370..2365)
                     /locus_tag="PKH10_10520"
                     /inference="COORDINATES: protein motif:HMM:NF019274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor family
                     protein"
                     /protein_id="HNT28597.1"
                     /translation="MRAIATIAAVLFIIACGDETKPVPDTGKTTAQTLFDVYKDYTEN
                     GESTPEGNPNDPSTGMPCDEKDGVDTNGDGVITNCDDNTTVPGDPGQPDGGYGVMPLC
                     PEIIGLNFDTCRPEMGRPLFIIDFGRACVLGMKCEYNGTEPACPMVWAPVCGVDGWTY
                     DNDCFAKLAGVEIAYEGECWGGPNGPECPDLAYPPEDFCPNGKIIPVYDKELGCQVAW
                     DCNNENNPMYCNADSDCDAGMLCVNGICSYAEPDCPVGMPNPEDYCPDETKMMPIYDE
                     KGCVTDWYCEGGTDPNDPTIDCPAIDLKMPDCGENAKAYPIYDAAGCQTGWSCSL"
     gene            complement(2500..>2937)
                     /locus_tag="PKH10_10525"
     CDS             complement(2500..>2937)
                     /locus_tag="PKH10_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28598.1"
                     /translation="PDLAARLAALAGRLAQFGAESREEEAIQAHMVAALYALRRAFAW
                     ETTGGAATPGTPCEELCRVAAELRDGETTGEGPWLAGFYLAAAEQRLSLIPPKLKSYR
                     KVEAQGDVPKRRSVKLATQTSRVEQIAAAVTRLEEFLNILLPE"
BASE COUNT          494 a          922 c          862 g          659 t
ORIGIN      
        1 gcgggcgcgc gtagaaggtt gacatagccg ttgctccacg acccgccgcg caacacgcgg
       61 aaaccaccgg tttctggccc cgtggggttg tttgcgggcg atgatgtgta gtaatctaag
      121 ccatgccagt cgttcaccca ctcccacaca ttgcccgcca tgtcgtaagc gccatagggc
      181 gacttgccca cctccttgct tcctatcggc atggtcccgc cactaccaca tccctcgcca
      241 ccggcattgt cgtcatccat taccgcgtaa tcacaggtcg catcttcgtt gccccacggg
      301 tatttccgcc cgtcagtgcc gcgcgccgcc ttctcccatt ccgcctcggt aggaaggcga
      361 ccgcctatcc actcgcagta ggcctttgcg ccataccatg tcacgcagtt catcgggtgg
      421 tttccttttc cttccgcgtc gtaattgcaa tcaccgctgt ccgtgttggt gtaatagtga
      481 tgatactcgc cgctgttgtt gcacacgccg ttggtgacgc actggtgata ttcgccgacg
      541 gtcacttcgt acttgccgat cttgtatgcg gagagcgtca ccgcatgata cggtgattcg
      601 tcactctcac attgaaggtc caccgctgaa ttacagccca tctggaattc accggcgggg
      661 acatccacca tctcgcccgg cgtgccagcg tcgtagtcaa catcgggttg ttcagtgtca
      721 gtcgcggatg tgtctttgtc gggcgtttcg gtaccatctc ctccacacgc cgccagaagg
      781 aacatagcca caaggatcat aatgtgcttg gtcatggggg tccccctctg gtgaatgtcg
      841 cggcctcgat tgtatccccc cgaagaaacg aagcaagaaa acactcgccc tcttgaggct
      901 gcaagcctag acgcaagggc tatgatcgtt ttttgcggcg ccgctagtcg ccgatgatct
      961 tgacgaggac ccgtttgcgg cgcatcccgt cgaattcacc gtagaatatc tgttcccacg
     1021 gaccgaagtc tagtttgccg ccggtgatgg ccaccaccac ctcgcgcccc atcacctgcc
     1081 gtttgagatg cgcgtcggcg ttgtcctcgg cgccgttgtg ccggtactgg gcatgcggtt
     1141 tttcgggggc gagtttctcc agccagacct cgtagtcgtg gtgcagaccc cgttcgtcgt
     1201 cgttgatgaa gacgctcgcg gtgatgtgca tcgcgttgac gagcaccagc ccctccttca
     1261 cgccgctctc ggcgaccgcc cgttcgacat cgggagtgat gttgatgaag gcgcgtcgcg
     1321 tcgtcgtggt gaaagacagt tccttgcggt gatgtttcat cggaagaatt cagagcgagc
     1381 aggaccagcc ggtctggcag ccggcggcgt catagatagg gtaagctttc gcgttctcgc
     1441 cgcagtcggg catcttgagg tcgatggcgg gacagtcgat ggtcgggtcg ttcggatcgg
     1501 tgccgccttc acagtaccaa tccgtcacgc agcccttctc gtcgtatatc ggcatcatct
     1561 tggtctcgtc ggggcagtaa tcctcggggt tcggcatgcc gaccggacag tcgggctcgg
     1621 cgtaggagca tatcccgttc acacagagca tgcccgcatc gcagtcgctg tcggcattgc
     1681 agtacatcgg gttattctca ttgttgcaat cccacgcgac ctgacagccg agttccttgt
     1741 cgtacaccgg gatgatcttg ccgttcgggc agaaatcctc gggcggatag gcgagatcgg
     1801 ggcattcagg cccgttgggg ccgccccagc attcgccctc gtaggcgatc tcgacccccg
     1861 cgagtttagc gaagcagtcg ttgtcgtagg tccagccgtc aacgccgcag acgggcgccc
     1921 acaccatcgg gcaggccggt tcggtgccgt tgtattcgca cttcatgccg agtacgcagg
     1981 cgcgaccgaa gtcgatgatg aagagcggac ggcccatctc gggacggcag gtgtcgaagt
     2041 tgagcccgat gatctcgggg cagagcggca tcacgccgta gccgccgtcg ggctggccgg
     2101 gatcgccggg aacggtggtg ttgtcgtcgc agttggttat cacgccgtca ccgttggtgt
     2161 ccacgccatc cttttcgtca cacggcatac cggtgctcgg gtcgttcggg ttcccctcgg
     2221 gggtcgactc gccgttctcc gtatagtcct tgtagacgtc gaatagggtc tgggcggtcg
     2281 ttttcccggt atcgggtacc ggtttggtct cgtcgccgca agcgatgatg aacagcacgg
     2341 cggcgatggt ggcgatggct ctcatgactc tcctcctaaa agatcggtta tgatcgaacg
     2401 ctgacagccc tgtagtacca ccattgtagc ctatttattg gaaagtcaag cgaaaatatt
     2461 tcctatttgt tgaaataact tggatttctc gtagccgccc tattcaggca gcaggatatt
     2521 gaggaactct tcgagccgcg tgaccgccgc cgctatctgc tcgacgcgtg atgtctgcgt
     2581 cgccagtttc accgaccgtc gcttgggcac atcgccctgc gcctcgacct tccggtaact
     2641 tttaagtttc ggcgggatga gtgacagtct ctgttcggcc gccgcgagat agaaccccgc
     2701 gagccacggt ccctcgcccg tcgtttcgcc gtcgcgcagt tcggccgcga cgcggcatag
     2761 ttcctcgcac ggggtgcccg gcgtcgccgc gccgcccgtc gtttcccatg caaaagcccg
     2821 ccgcagggcg tagagcgccg ccaccatgtg cgcctgtatc gcctcctcct cccgactctc
     2881 ggccccgaac tgggcaagcc gtcccgcaag cgccgcgagc cgcgcggcaa ggtcggg
//
LOCUS       DAOQUE010000373         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_39642_length_2934_cov_3.925321, whole genome shotgun sequence.
ACCESSION   DAOQUE010000373 DAOQUE010000000
VERSION     DAOQUE010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39642_length_2934_cov_3.925321"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKH10_10530"
     CDS             <1..247
                     /locus_tag="PKH10_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNT28599.1"
                     /translation="GGMYWDSYNTIKRLDDYLPVDVYVAGCMPRPESLLAGFEELKRI
                     IREGKGEGANKYAAEFDRYKANQKQVIHDWDMPDYNW"
     gene            254..784
                     /locus_tag="PKH10_10535"
     CDS             254..784
                     /locus_tag="PKH10_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNT28600.1"
                     /translation="MRELYEQLKRLFPLGEYTEQRRDLAFVTVPKDHLRPLLIHLRDR
                     ERFSHLVLLTAVDWLEEGHFQLTYLMNDRAGKRDLGLRVLIPRDGAAMEGIHDLWPTA
                     ATYQRELREMFGIDFPGSPRVNEEFILEGWTGMPPYRRDFDTKKFSEENFNHRPGRAT
                     TDPATHMKEKLYPDEA"
     gene            786..1964
                     /locus_tag="PKH10_10540"
     CDS             786..1964
                     /locus_tag="PKH10_10540"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNT28601.1"
                     /translation="MALYEPDRSQYPVKKPDGTLDIDLSSGKYLKLWQGPQHPGITGN
                     MSLELTVCGDEVVDCQTHVGYLHRGFEKLMERRTYIQCFPIVCRICVPEPDFNEYCYA
                     AAVEELSGIKSPHRADWIRTLILEMGRINSYMMYLGGHAGSLGMGVIGQWTTYVRDLM
                     LDRFEELTGARIYHMFILPGGVRDGLPDGFEKRMEETLREIESTMKDVYEAMFCNAVF
                     KKRAIGLGYIDPALIDPYGVTGPTARAAGVAKDVRKDFPYLVYPELDFEPVMGKDSDA
                     FTRADVRRRDVLLSIDLIRQILAKMPHDGPLMAKIPNVLHWKVPRGETYIRAECSRGE
                     YGYYLVADGSGYPRRVNVRGPSYTHAVALLERLIINVNIADVAGLMVSLHTYPPEIER
                     "
     gene            1968..>2934
                     /locus_tag="PKH10_10545"
     CDS             1968..>2934
                     /locus_tag="PKH10_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT28602.1"
                     /translation="MKIRDILSPFTVWKNLFRDPVTIDDPVRQRPGAPRYRGFHKNDV
                     KKCIGCGTCETICQNGAIDMVPVDGIAAKDGDSGLRPRIDYGRCCWCALCVDVCMTKS
                     LTMSNDYQWIDRDPDAFRFTPGVDKKKWDDAELGYKRPDEHRLTPEERVHMQELGPEA
                     RVGSFIEIVKGYSVEEARKEADRCVACGLCTATCPAHMAIPDYIAAIRDGDYERGLKL
                     LYDTHPLPEVCGRVCTHKCESVCAAKHEGDPIAIRWLKRHIADQVPLDKYRAIVGAAE
                     PATGKKVAVVGAGPAGLSAAYYLARMGHAVNPAGPAPTTATFFPVA"
BASE COUNT          587 a          997 c          875 g          475 t
ORIGIN      
        1 cggcgggatg tactgggact cctacaacac catcaagcgg ctcgacgact acctgccggt
       61 cgatgtctat gtggcgggct gtatgccgcg tcccgaatcg ctactggcgg ggttcgagga
      121 actcaagcgg atcatccgcg aagggaaagg cgaaggggcc aacaagtatg ccgctgagtt
      181 cgaccgctac aaggcgaatc agaagcaggt gatacacgac tgggatatgc ccgattataa
      241 ctggtaggga acgatgcgtg aactctatga acagctgaaa cggctcttcc cgctcgggga
      301 gtataccgaa cagcgccgcg atctcgcctt cgtgacggtg ccgaaggatc acctgcggcc
      361 gctcctcatc cacctccgcg accgtgaacg cttctcccat ctggtcctcc tgaccgccgt
      421 cgattggctt gaagaggggc acttccagct cacctatctc atgaacgacc gcgccgggaa
      481 acgcgatctc ggtctccggg tgctcatccc gcgcgacggc gcggcgatgg aaggtatcca
      541 cgatctctgg ccgaccgcgg cgacctacca gcgcgaactg cgcgagatgt tcggcatcga
      601 cttccccggc agcccccgcg tgaacgagga gttcatactg gaagggtgga ccgggatgcc
      661 cccctaccgg cgcgacttcg acacgaagaa attctccgaa gagaacttca accaccggcc
      721 gggacgcgcg accacagacc cggcgacgca tatgaaagaa aagctttacc ccgacgaggc
      781 gtgacatggc gctctacgaa cccgaccgca gtcagtaccc ggtgaaaaag ccggacggga
      841 cgctcgacat cgacctctca tcgggcaaat acctgaagct gtggcagggc ccccagcacc
      901 ccggaatcac cggcaacatg tcgctggaac tgaccgtctg cggcgacgag gtggttgatt
      961 gccagaccca tgtcggctac ctgcaccggg gcttcgagaa actgatggag cggcgcacct
     1021 acatccagtg cttcccgatc gtctgccgca tctgcgtccc cgaaccggac ttcaacgaat
     1081 actgctacgc cgcggcggtc gaggaacttt cgggcatcaa atcaccgcac cgcgccgatt
     1141 ggatacggac cctcatcctc gagatgggac ggatcaacag ctacatgatg tacctcggcg
     1201 gacacgcggg aagcctcggc atgggggtca tcggccagtg gaccacctat gtccgcgacc
     1261 tgatgctcga ccggttcgag gagttgaccg gtgcccgcat ctaccacatg ttcatcctgc
     1321 cgggcggcgt ccgcgacggc ctgcccgacg gctttgagaa gcggatggaa gagacgctcc
     1381 gcgagatcga gagcacgatg aaggatgtct acgaggcgat gttctgcaac gccgtcttca
     1441 agaaacgggc catcgggctc ggctacatcg acccggcgct catcgatccc tacggcgtca
     1501 ccgggccgac cgcccgcgcc gccggggtgg cgaaggatgt ccgcaaggat ttcccgtatc
     1561 tcgtctaccc cgaactcgac ttcgagccgg tcatggggaa ggattccgac gccttcaccc
     1621 gcgccgatgt ccgccgccgc gatgtactcc tctccatcga ccttatccgg cagatactcg
     1681 ccaaaatgcc gcacgacggc ccgctcatgg cgaagatacc gaatgtcctc cactggaagg
     1741 tgccgcgcgg cgagacctac atccgcgccg aatgttcgcg cggcgaatac ggctactacc
     1801 tcgtcgccga cggttcgggc tacccgcgcc gcgtgaatgt ccgcggcccc tcctacaccc
     1861 acgccgtcgc cctgctggag cggctgatca tcaatgtgaa catcgccgat gtcgccgggc
     1921 tcatggtatc gctccacacc tatccgcccg agatagagag gtaacggatg aagatacgcg
     1981 acatcctctc acccttcacc gtctggaaga acctgttccg cgatccggtc acgatagacg
     2041 atccggtccg ccagcgcccc ggcgcgccgc gctaccgcgg cttccacaag aacgatgtca
     2101 agaagtgcat cggctgcggc acctgcgaga cgatctgcca gaacggcgcc atcgacatgg
     2161 tgccggtcga cggcatcgcg gccaaggatg gcgattcggg gctccgcccg cgcatcgact
     2221 acggtcgctg ttgctggtgc gccctgtgcg tcgatgtctg catgaccaag tcgctcacga
     2281 tgtcgaacga ctaccagtgg atagaccgcg atcccgacgc cttccgcttc acccccggcg
     2341 tcgacaagaa gaagtgggac gatgccgaac tcggctataa acgacccgac gaacaccgcc
     2401 tcacccccga agaacgcgtc catatgcagg aactcggccc cgaagcgcgc gtcggctcgt
     2461 tcatcgagat cgtgaaaggt tattcggtcg aggaggcgcg caaggaggcc gaccgctgcg
     2521 tcgcctgcgg cctgtgcacc gccacctgcc ccgcccacat ggcgataccc gactacatcg
     2581 ccgcgatccg tgacggcgat tacgagcggg ggctgaaact gctctacgat acccacccgc
     2641 tccccgaggt ctgcggacgg gtctgcaccc acaaatgcga atcggtctgc gccgcgaagc
     2701 acgagggcga ccccatcgcg atacggtggc tcaaacgcca catcgccgat caggtgccac
     2761 tggacaaata ccgcgccatc gtcggcgcgg ccgaaccggc caccgggaag aaggtcgccg
     2821 tcgtcggcgc cggcccggcg ggattgtcgg ccgcctacta cctcgcccgg atgggccacg
     2881 ccgtcaatcc cgccgggccg gcgccgacga cggcgacctt cttcccggtg gccg
//
LOCUS       DAOQUE010000374         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_39668_length_2933_cov_4.021195, whole genome shotgun sequence.
ACCESSION   DAOQUE010000374 DAOQUE010000000
VERSION     DAOQUE010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39668_length_2933_cov_4.021195"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /gene="secF"
                     /locus_tag="PKH10_10550"
     CDS             <1..983
                     /gene="secF"
                     /locus_tag="PKH10_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNT28603.1"
                     /translation="EIQIKIEGIDIGGVRSALSEFNGVEIMQFEGHDNEFTIKLPSIA
                     LTDATTVERYITDVQKAFPDTPLIKKHFNVEVGDRIELWFDKPIDQDRLTGMAKDLAV
                     PVTGQTEYNQVGERHIYKVFLKSFTSTVIAAVEKKSGSQAEVQRVELVGPKVGKKLRT
                     DTFMAVFYTLIALLIYVALRFNFQSAPGAVVALFHDTMITLGILVLFRVTFDLTIVAA
                     LLTMIGYSLNDTVVIYDRIRENLKNPHKGSTLSEKINISLNEVLSRTIITSGMTLLVV
                     LALLLLGGDIIWGFAFTMCIGIVVGTYSSIYIASPVTIFIERHMKKEQAT"
     gene            988..2709
                     /gene="recJ"
                     /locus_tag="PKH10_10555"
     CDS             988..2709
                     /gene="recJ"
                     /locus_tag="PKH10_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNT28604.1"
                     /translation="MKKEWVLLDADPAKVNTITKTLGISEPVANILVNRGLDDLESAS
                     LFLQAKLKNLPDPSLIKDAQKASKIIVEAIKKGRHIVIYGDYDVDGITSTVLVYKFLS
                     VLGASVRCYIPHRIEDGYGLNTYTLEEIATDGGEVVLTVDCGITSVEEVRYARNLGLD
                     IIIVDHHHIGETLPDAVAIINTHAEDYPYPFKEMAAVGVAFSFLMVLKKAVEEDGFFT
                     GTLPNLKEYMDLVALGTVADMVPLVGTNRIFVKHGLQQMQTNNRPGLLALAHVCGLKS
                     LDEVTPAIISYKMAPRLNAAGRIGNAIKGVDLLLAQNYDVAIKMAEDLNLTNEYRQKL
                     EAEIFEQAIRLVEEKRLHEKNALVLYSHNWHPGVIGIVASRLVERYYKPTIMISVEGG
                     VGRGSARSIQKLHIYHVLEQLNGLLVQYGGHKFAAGLTINEANIPEFARRFDEIVGGT
                     LTPDDYQNVLEIDAQLELAKLTPELVVALSSLEPFGMNNWEPNFLATRLVVKKQNVVN
                     RAHLHWRFQAEDCPLQFNAIGFGLANERQVPSIGDRIDIVYFPRINVFNGSVSLRLYV
                     KDFRLSS"
     gene            complement(2724..>2933)
                     /locus_tag="PKH10_10560"
     CDS             complement(2724..>2933)
                     /locus_tag="PKH10_10560"
                     /inference="COORDINATES: protein motif:HMM:NF037773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28605.1"
                     /translation="VGDIGRDPQVRYDTFRRMDEAAGTYQVVYGRDGRLVGGVLIGDI
                     RRAGAISKGIPQGLAFTEAERLLAG"
BASE COUNT          680 a          921 c          752 g          580 t
ORIGIN      
        1 ccgagataca gataaagata gagggcatcg atatcggcgg tgtccgttcg gccctttccg
       61 aattcaacgg tgtcgagatc atgcagttcg agggtcacga taacgaattc accatcaaac
      121 tcccctcgat agcacttacg gacgccacca ccgtggaacg ctatatcact gatgtccaaa
      181 aggcgttccc cgatacgcca ctcatcaaga agcatttcaa tgtcgaggtc ggcgaccgca
      241 tcgaactgtg gttcgacaag ccgatagacc aggatcgcct gaccgggatg gcaaaggatc
      301 tggcggtgcc cgtcaccggg cagacagaat ataatcaggt cggcgaacgc cacatctata
      361 aagtgttcct taagagtttt acgagcaccg tcatcgcggc ggttgaaaag aaaagtggtt
      421 cacaggccga ggtacagcgg gtcgaactcg tcggccccaa ggtcggcaaa aaactgcgaa
      481 ccgacacttt catggctgtt ttctacaccc tcatcgctct gctcatctat gttgctctcc
      541 gtttcaactt ccaatcggcc cccggcgccg ttgtcgcgtt gttccacgac accatgataa
      601 cgctcggcat actggtactg ttccgcgtca ccttcgacct caccatcgtc gccgcgctcc
      661 ttacgatgat cggctactct ctcaacgata ccgtcgtcat ctacgaccgc atccgagaga
      721 acctcaagaa cccgcacaag ggatcgacac tgagcgagaa gataaacatc agcctcaacg
      781 aggtcctcag caggacgatc atcacctccg gcatgaccct gctcgtcgtg ctcgcgctcc
      841 tgctcctcgg cggcgacatc atctggggct tcgctttcac gatgtgcatc ggcatcgtcg
      901 tgggcaccta cagttccatt tacatcgcca gcccggtcac gatctttatt gagagacata
      961 tgaagaaaga acaggcgacc tgagcggatg aagaaagaat gggtactact ggatgctgac
     1021 cccgcgaaag taaacacgat caccaagacg ctcggcatat ccgagccggt ggcgaacatt
     1081 ttggtgaacc gtggacttga cgacctcgag tcggcaagtc tctttctgca ggcgaaactc
     1141 aagaatctgc ccgacccctc cctcataaaa gacgctcaga aggcctcgaa gatcatcgtc
     1201 gaggcgataa agaagggccg acatatcgtc atctacggcg attatgatgt ggacggcatc
     1261 acctcgaccg ttcttgtcta caaattcctt tcggtgctcg gcgcgagcgt ccggtgctat
     1321 atcccccacc gcatagagga cgggtatgga ctcaacacct atacgctcga agagatagcc
     1381 accgacggcg gcgaagtggt gctcaccgtc gattgcggca tcacgagcgt cgaggaggtc
     1441 cggtacgccc gtaatctcgg actcgacatc atcatcgtgg accaccacca tatcggcgaa
     1501 acgcttccgg acgcggtcgc catcatcaac acccacgcgg aggactaccc ctaccccttc
     1561 aaggagatgg cggccgtcgg tgtcgccttt tccttcctca tggtgctcaa aaaggcagtg
     1621 gaggaggacg gctttttcac cggcacgctc cccaacctca aggaatatat ggacctcgtc
     1681 gcgctcggca ccgtcgccga catggtgccg ctcgtcggga ccaaccgtat cttcgtcaag
     1741 cacggactcc agcagatgca gacgaacaat cggccgggac ttctggctct cgctcatgtg
     1801 tgcggtctca agtcgctcga cgaggtgacc cccgcgatca tcagttacaa aatggcgccc
     1861 cgtcttaacg cagcgggccg tatcggcaac gccatcaagg gggtcgatct cctgctcgcg
     1921 cagaattacg atgtcgcgat aaagatggcc gaggacctca atctcaccaa cgagtaccgc
     1981 cagaaacttg aggcggagat attcgaacag gcgatccggc tggtcgaaga gaagcggctc
     2041 cacgaaaaga acgccctggt actctattcg cacaactggc accccggcgt catcggtatc
     2101 gtcgcctcgc gactggttga acgatactac aagccgacca tcatgatatc ggtcgagggc
     2161 ggcgtcggac gcggatcggc ccgttcgata cagaaactgc acatctacca cgtccttgaa
     2221 caactcaacg gcctgctcgt gcaatacggc ggccacaagt tcgccgccgg ccttaccatt
     2281 aatgaggcga atatccccga gttcgcccgc cgtttcgacg agatagtcgg cggaaccctc
     2341 acccccgatg actaccagaa tgttctcgag atagacgcgc aactcgaact ggcgaaactg
     2401 accccggaac tcgtcgtcgc tctctcatct ctcgaaccgt tcggcatgaa caactgggaa
     2461 ccgaacttcc tcgccacccg gctggtcgtg aaaaaacaga acgtcgtgaa ccgcgcccac
     2521 ctgcactggc gattccaagc ggaggattgc ccgttgcagt tcaacgccat aggcttcgga
     2581 ctcgcgaacg aacgacaggt cccctcgatc ggcgaccgga tagatatcgt ctatttcccg
     2641 cgcatcaatg tcttcaacgg tagcgtttct ttacgactgt atgtgaagga tttccggcta
     2701 tcgtcctgaa tatctttcga gcatcagccc gccaacagcc tttcggcctc ggtgaacgcc
     2761 agcccctgcg ggataccttt ggatatcgcc ccggcccgac ggatatcgcc gataagcact
     2821 cccccgacaa gccgtccatc acggccatac acgacctgat atgtccccgc agcctcatcc
     2881 atgcggcgga aggtgtcgta ccgcacctgc ggatcgcgcc cgatatcccc gac
//
LOCUS       DAOQUE010000375         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_39670_length_2933_cov_3.390896, whole genome shotgun sequence.
ACCESSION   DAOQUE010000375 DAOQUE010000000
VERSION     DAOQUE010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39670_length_2933_cov_3.390896"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..88)
                     /locus_tag="PKH10_10565"
     tRNA            complement(1..88)
                     /locus_tag="PKH10_10565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Ser,seq:cga)
     gene            complement(89..550)
                     /gene="tadA"
                     /locus_tag="PKH10_10570"
     CDS             complement(89..550)
                     /gene="tadA"
                     /locus_tag="PKH10_10570"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018923319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNT28606.1"
                     /translation="MERDGHFMREALALARRAEQRGEVPVGAVVVLDNRIVGIGYNRR
                     ECTQSPLAHAEVLAIEDARDQIGFWRLERCELFVTLEPCVMCCGAIIQSRIARVCFGA
                     RDPKGGGVVSLYTLLTDPRLNHRCEVVEGVLGDECGTMLTDFFRNIRAISK"
     gene            797..1153
                     /locus_tag="PKH10_10575"
     CDS             797..1153
                     /locus_tag="PKH10_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HNT28607.1"
                     /translation="MKEQALTKMKEVMHAVYSFENHLIKNHALLLNAALTLCALEGSK
                     AKRSSDLAEELGVTYSLMSRTVVLLEKRSLVDRSIGKTDKREMRFTLTEKGKRLLKEI
                     REGYDLGKFLSTLKSR"
     gene            complement(1165..2193)
                     /locus_tag="PKH10_10580"
     CDS             complement(1165..2193)
                     /locus_tag="PKH10_10580"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNT28608.1"
                     /translation="MSEAQKVDLNWSKLGFSYIRTDKRFIAHWKNGYWEKGTLTEDNK
                     LHIDEGACALHYGQQCFEGLKAFTTKDGRTVLFRPDQNAKRMQRSANALLMVAVPDEM
                     FIEACEQVVRANLKWIPPYGHGASFYLRPFLIGVGENLGVRPAPEYIFSVFGSPVGPY
                     FKGGLQPVKFSVSEYDRAAPNGTGSVKVGGNYAASLHAHEIAVKAGYADCIYLDPSTH
                     KNIEEVGAANFFGITKDNLFVTPKSNSILPSITKYSLMHIAEHYLKMRVEERDVPIDR
                     LDDFVEAGACGTAAVITPIGGIFHNGKLHTFYADGKEVGPVSRKLYDLLTGMQTGTVE
                     APQGWSTR"
     gene            complement(2280..2729)
                     /locus_tag="PKH10_10585"
     CDS             complement(2280..2729)
                     /locus_tag="PKH10_10585"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNT28609.1"
                     /translation="MVDLVCTFIFLGAMFAGYLAGGFREILKVGVMIGLMVLFMLPSV
                     KAVFLQFGPAANGIFIVSFLAVYVAASWLVMWALKGLVESQEGVIGGLNKAIGIIAGF
                     FKATLLIVFAAYITRFLWSKKMLAEVKPHLDDSFIFSIASEILDFFL"
BASE COUNT          636 a          863 c          823 g          611 t
ORIGIN      
        1 ggagagatag ggattcgaac cctaggtacc cctcgcggag cacacttgat ttcgagtcaa
       61 gctccttcga ccactcggac atctctcctc acttcgatat cgccctgata ttcctaaaga
      121 aatcggtcaa catcgtgccg cattcatcac ccaagacccc ttccacgacc tcgcaacgat
      181 ggttcaggcg cggatccgta agcagggtat agagcgatac cacgccgccc cccttggggt
      241 cacgcgcacc gaaacagacc cgggcgatgc gggactggat gatcgcacca cagcacatga
      301 cgcacggctc tagggtgacg aagagttcac atcgttcgag acgccagaaa ccgatctgat
      361 cacgggcatc ctcgatagcg agtacctcgg catgggcaag cggcgactgg gtacattcgc
      421 ggcgattgta cccgataccg acgattcgat tgtcaaggac caccaccgct ccgaccggca
      481 cctcaccccg ttgctccgca cggcgagcaa gagcaagcgc ctcgcgcatg aagtgtccgt
      541 ctctctccat cggctccggt acggtcaaag cggggtcatt atagtgcgaa aaggggcctt
      601 gtcaatttca gggacacatc tttacgaaaa ccggctttcc gacgacaaaa aacggcggaa
      661 cacctccgct ttttttctct tttttccatt cagcaccgat catgtcgggc gtttttcgcg
      721 gcccgcttta tgcttatccc agaaaagaaa cgcaaaagaa gaaagcagtc gttcaaccac
      781 gaaggagagg aacaccatga aagaacaggc gctcaccaag atgaaagagg tcatgcacgc
      841 cgtctattcc ttcgagaacc atctcatcaa aaatcacgcg ctgctcctca acgccgctct
      901 cacgctttgc gccctcgaag gaagcaaggc gaaacgcagc agtgatctgg ccgaagaact
      961 cggcgtgacc tattcgctca tgtcccgcac ggtcgtcctg ctcgagaaac ggtcgcttgt
     1021 cgatcggagc atcgggaaga ccgataagcg ggagatgcgt ttcaccttga ccgaaaaagg
     1081 gaaaagactg cttaaggaga tccgggaagg atacgatctc ggcaaattcc tttctacgct
     1141 caagagccgg taacggcggt cacttcacct cgtggaccag ccctgcggcg cttcgacggt
     1201 gccggtctgc atgccggtca gcagatcata gagtttccgt gagaccggcc cgacctcttt
     1261 gccgtcggca tagaaggtgt gcagtttgcc gttgtggaat atgccgccga tcggggtgat
     1321 aaccgccgca gtgccgcagg cgcccgcctc gacgaaatcg tcgagccggt caatcgggac
     1381 atcgcgctcc tcgaccctca ttttcaggta gtgttcggcg atgtgcatga gcgaatactt
     1441 ggtgatgctc ggcaatatcg agttcgactt cggtgtgacg aagaggttgt ccttggtgat
     1501 gccgaagaag ttggcggcac cgacctcctc gatgttcttg tgggtcgacg ggtcaaggta
     1561 gatgcagtcg gcgtagcctg ccttgacggc tatctcgtgg gcgtggaggc ttgcggcgta
     1621 gttgccgccc accttcaccg atccggtgcc gttgggtgcc gcgcggtcgt attccgacac
     1681 gctgaacttg accggctgga gaccgccctt gaaatagggg ccgaccgggg agccgaagac
     1741 cgaaaaaatg tattcgggcg cgggacggac gccgaggttc tcgccgacgc cgatcaggaa
     1801 cgggcgcaga tagaacgacg cgccatgacc gtagggcggt atccatttca ggttagcgcg
     1861 gaccacctgt tcgcacgcct cgatgaacat ctcgtcgggg accgctacca tgagaagtgc
     1921 gttggcggag cgctgcatcc gcttggcgtt ctgatcggga cggaacagca ccgtgcggcc
     1981 gtccttggtc gtgaaggcct tgagcccctc gaagcactgc tggccgtaat ggagcgcgca
     2041 agccccctcg tcaatgtgca gtttgttgtc ttcggtgagc gtccctttct cccagtagcc
     2101 gttcttccag tgcgcgatga agcgtttgtc cgtcctgatg taggaaaatc ccagtttcga
     2161 ccagttgaga tcgacctttt gtgcctcgct catggctctc tcctaaaaaa agtatggacc
     2221 gttccctcac ggaacgctat cgaggctccg aaggtttgtc aaggatagcg acatgccacc
     2281 tagagaaaga aatcgagtat ctcactcgct atcgaaaaga tgaaagagtc gtcgagatgt
     2341 ggtttcacct cggcgagcat ctttttcgac cagaggaagc gggtgatgta ggcggcgaac
     2401 actatgagga gggtcgcctt gaaaaatccg gcgatgatgc ctatcgcctt attcaggcct
     2461 ccgatgaccc cttcctgcga ctcgacgagc cccttgagcg cccacatgac cagccatgat
     2521 gcggccacat agaccgcaag gaacgaaacg atgaatatgc cgttggcggc gggcccgaac
     2581 tgcaggaaga ccgctttgac cgagggaagc atgaacagga ccatgagccc gatcatgacc
     2641 cccaccttga gtatctcccg gaacccgccg gcgaggtagc ccgcgaacat cgccccgaga
     2701 aaaatgaagg tgcagaccag atcgaccatg ccggccctca aaaaaagacg ggggaccgat
     2761 aacgatcccc cgccggaatt cctgtcacgc tatcgcgggt cattcgtctt tcttcttggc
     2821 actctttttc ttggccggtt ctttttcttc ggccttatcc tttttcgcct tggcctcctt
     2881 cttgggggcc tcctccttct tggcgggcgc cttctttacg gcgggctttt tcg
//
LOCUS       DAOQUE010000376         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_39850_length_2925_cov_4.233101, whole genome shotgun sequence.
ACCESSION   DAOQUE010000376 DAOQUE010000000
VERSION     DAOQUE010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39850_length_2925_cov_4.233101"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKH10_10590"
     CDS             complement(<1..322)
                     /locus_tag="PKH10_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT28610.1"
                     /translation="MEGWDLQRAAALVTAHLHTVNMVKHSWAVEAGMRRMAEHLGEDA
                     DRWALTGLLHDLDYEMTKDDFPRHGIVSGEILRKEGFADGAVLAAIIEHTGNTPAESR
                     MGRAL"
     gene            489..564
                     /locus_tag="PKH10_10595"
     tRNA            489..564
                     /locus_tag="PKH10_10595"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:522..524,aa:Val,seq:gac)
     gene            596..2508
                     /gene="thrS"
                     /locus_tag="PKH10_10600"
                     /pseudo
     CDS             596..2508
                     /gene="thrS"
                     /locus_tag="PKH10_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
     gene            2505..>2925
                     /gene="infC"
                     /locus_tag="PKH10_10605"
     CDS             2505..>2925
                     /gene="infC"
                     /locus_tag="PKH10_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNT28611.1"
                     /translation="MTTTGPLDKEKDKGADQLVNESIPYREMRVISPEGEMLGVLAKR
                     DALEKARGYNLDLVCVSPNTTPVVCKIMDYGKYLYIMKKKAKEVKKKQTVIEIKELKI
                     RPNTDTHDLMVKVRQGKEFLKDGCKIKLTVFFRGREAM"
BASE COUNT          646 a          912 c          836 g          531 t
ORIGIN      
        1 agagcgctct ccccatccgg ctctcggccg gcgtgttgcc ggtgtgctcg atgatggcgg
       61 caagcaccgc tccgtcggca aagccctcct ttctcaatat ctctcccgac acgatgccgt
      121 ggcgcgggaa atcatccttg gtcatctcat aatcgagatc gtgcaaaagc ccggtcagcg
      181 cccagcggtc ggcatcctcg ccaagatgtt cggccatccg gcgcatcccc gcctcgaccg
      241 cccaactgtg tttcaccatg ttcaccgtgt gcaggtgagc cgtgacgagt gcggccgccc
      301 gctgcagatc ccatccttcc atggaacagg cctctctccg ttatctttca tcctttgtaa
      361 gactattctc acgagcgcca aaatcaagtt tccgccaccg ccggagaggt gtgagggcac
      421 ctctgaaaag attttgtttg acaagcgggg aaaatcggct atacattcgc ctgcttgttt
      481 cgacatgcgg gctcgtagct caattgggag agcactgctt tgacgtagca ggggtcagag
      541 gttcgaaccc tctcgggcct accatgtctt ttttttgtcc ttaagagttt tgggcatgat
      601 agtaacactt cccgacggat cacggaaaga ggcgaaagcg ggagagcact ggcgcgactt
      661 catcgcccgc gagatcggtc agggactcgc caagagcgcg ctcgccgtca agatcggcaa
      721 cgcggtgaag gatgtctcct ccccggtcga ggagggctcg ctcgtggtcg tcaccgcctc
      781 gtcgcccgac ggactcgaca tcatccgcca ctccgcgtcg cacatcatgg ccgatgcggt
      841 tacccaactc ttccccggca ccaaggtcac catcggcccc gcgatagaga acgggttcta
      901 ctacgacttc gattccgacc atcgtttcac cgccgaggat ctcgagcgga tagaggcgaa
      961 gatggccgag ataatcaagg cgaagcaacc cttcgcgcgg accgaactat cagcggacga
     1021 ggcgaaaagg agattctcgg aactcggcga gacctacaag gtcgagatca tacaggacct
     1081 cggcgcccag gtcgtatcgc tctacaccca cggacacttc accgatctct gccgcggccc
     1141 gcacctgccc gacaccgggt atgtgaaggc gcacaagatc ctgtcggtcg ccggcgccta
     1201 ctggcggggc gattccaaga acaagatgct ccagcgcatc tacggcaccg ccttcgccga
     1261 caaaaagtcg ctcgacgaac acctccacct gcttgaggag gcgcgcgaac gcgaccaccg
     1321 caaactcggg ccggaactcg atctgttcac catgaccgac gacatcggca tgggactcgc
     1381 gctctggacc cccaagggag gcgtcatccg caccgtgata gagaacttct ggcgccggaa
     1441 cattaccgcc acggctacga gatcgtctac acgccgcata tcgggcgcag ccgcctctgg
     1501 gaaacgagcg gccatctcgg cttctacaaa ggcaacatgt acagcccgat ggacatcgac
     1561 ggtgaggact actatgtcaa gccgatgaac tgcccgttcc acatcgaggt gtaccgtcgg
     1621 cgcaagtgga gttaccgcga gttcccgatc cgttgggccg aactcggcac cgtctaccgc
     1681 tacgaactgt cgggcgtgct caacgggctc aaacgggtcc gcggcttcac gcaggacgac
     1741 gcgcacatat tctgtcggct cgaccagttg cacgccgaga tcgcacgggt catcgatttc
     1801 tcgctgttca tcctgcgcgc ctacgatttc gaacagttca aggtctatct gagcacccgt
     1861 ccggcgaagg atttcgtcgg cgatgtcgcg gtgtgggata aggccgaagc ggcactcgat
     1921 tcggcactca aggcctccgg tctcccctac cagatcaacc cgggtgacgg cgccttctac
     1981 ggccccaaga tagatatcgc ggtcgccgac gccatcggcc gcgaatggca actgtcgacg
     2041 atacaggtcg acttcaacct gccggaacgc ttcgatctca cctatgtcgg gagcgacaac
     2101 gccgagcacc gccccatcat gatacaccgc gcgcttatgg gctcgctcga acggttcttc
     2161 ggggtgctca tcgagcacta caaaggggcc ttcccggtct ggctctctcc ggtgcaggtg
     2221 gcggtggtcc cggtgagcga gaaacatctc gacatcgcgg aaaaacttga aaatcggctg
     2281 aaaacgcata acattcgcgt tcttgtcgac cgccgcaacg agaaaacggg ctacaaggtc
     2341 cgcgagtggg tggtcgccaa gacgccgtac atcatcgtcg tcggcgacaa ggaagcgtcg
     2401 ctcgacacgg tgtctgtgcg gttgaggagc ggggagcagg tcgacatgac gctcgccgcg
     2461 ttcgaggaac aactggaccg cgagaaccgc ggaggagtct atttttgacg acgacaggcc
     2521 cgctggacaa ggaaaaggac aagggcgcag atcaactggt caacgagagc ataccgtacc
     2581 gcgaaatgcg ggtgatatcg cccgaaggcg agatgctcgg cgtgttggca aagcgtgacg
     2641 cgctcgagaa ggcgcgcggc tacaacctcg acctcgtctg cgtctccccc aacaccacgc
     2701 cggtcgtctg caagatcatg gactacggca agtacctcta catcatgaag aaaaaggcca
     2761 aagaggtaaa gaagaaacag acggtcatcg agataaagga actcaagatc cgccccaaca
     2821 ccgacaccca cgatctcatg gtcaaggtac ggcagggaaa agagttcctc aaggacgggt
     2881 gcaagataaa actcaccgtc ttcttccggg gccgcgaagc gatgt
//
LOCUS       DAOQUE010000377         2907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_40216_length_2907_cov_5.855540, whole genome shotgun sequence.
ACCESSION   DAOQUE010000377 DAOQUE010000000
VERSION     DAOQUE010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40216_length_2907_cov_5.855540"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1753
                     /locus_tag="PKH10_10610"
     CDS             <1..1753
                     /locus_tag="PKH10_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28612.1"
                     /translation="ACNLHTAKNDADTVTADDDMSHMEGDTLTSDDAAVSNDETIDEV
                     LSDNDAVVCPDLKVYENVKAAGFPFKDGNGKITFCRPGCDAPTENDPQCTRNLWDWMN
                     WYHYKQYLAGTNTLGGSYNYKECYPWPCVLPELKSVALTHSPCDRDVTPGADLSADRG
                     TLYDLRVENGMVGGEMKNGGVNTRTLFYDIEKDEFISIASSGTSTGYRAGRLIFYSTA
                     NDVMRYPEEGLGNTYIFSAKKTGMGYRYEVIYDDEQHQADFSRPPLVGEKWVVLNIRH
                     RATGSYEVLYSKVDEWTWHSLTYSKVYEGNVVDNYLAFINDNREVYVCDLDKLPYNPA
                     KECIRIDREGEKAYQPRLNEENKNQLVYFAGVGEYGITFVDLSNRTPQYTLLPITPSD
                     PQPLGTYPHQFKGNLILYSELFLPPNEEIREDFKACFYRLDTKKQYCPKTPAFEDGRY
                     DMGFNSFDGNYQLWKAPASINARFRDIACYCEKEGICPFEGMTVKSPVPRPTLRSTKQ
                     TKTTPPEPLFARTTEDTCRRIIANESWDKEGYCGPLGVDAIFRSSTCPPLSGDPKKAF
                     SECVEWLSRSRAFSELP"
     gene            2083..2223
                     /locus_tag="PKH10_10615"
     CDS             2083..2223
                     /locus_tag="PKH10_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28613.1"
                     /translation="MTRFLIIAAAVAIAVSCATEQKDYSKEQEKAREHGQEAFQAPEV
                     NE"
     gene            2336..>2907
                     /locus_tag="PKH10_10620"
     CDS             2336..>2907
                     /locus_tag="PKH10_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014002379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNT28614.1"
                     /translation="MFDTLSEKLELTFRRLRGVGKLSEDNIQEAVREIRLSLLDADVN
                     YGVVKQFLEKVSARAVGAEVTRSVTPGQQFVKVCHDEMVALLGAGAHELNLAVRPPAV
                     ILMAGLQGSGKTTTAAKIGRHLKEKLRKRVLLVPADIYRPAAIDQLKKLGETIGIDVF
                     DSKPGDDPLRIAEAAMKQARDGVYDVVIVDT"
BASE COUNT          706 a          797 c          775 g          629 t
ORIGIN      
        1 cgcctgcaat ctacacaccg caaagaatga cgccgatacg gtgaccgcgg acgacgacat
       61 gtcgcacatg gagggcgaca ccttaacgag cgacgatgcc gctgtttcta acgatgagac
      121 catcgacgag gttctttccg ataacgatgc tgttgtctgt cccgatctca aggtctatga
      181 gaatgtcaag gcggccgggt tcccgttcaa ggatggtaac gggaagataa ccttctgtcg
      241 gccgggatgc gatgcgccga ccgaaaatga tccgcaatgt acgaggaatc tctgggattg
      301 gatgaattgg taccattata aacagtatct ggcggggaca aacaccttgg gaggaagtta
      361 caactacaag gaatgctatc cgtggccgtg tgttctgccg gagttaaaaa gcgtagctct
      421 gactcacagc ccgtgtgatc gcgatgtgac gcccggcgca gatctctcgg cagacagggg
      481 gacattgtac gatcttcgtg tagaaaacgg catggtgggc ggcgaaatga agaacggcgg
      541 tgtcaatacg cgcaccctat tctatgacat tgaaaaagac gaatttatca gtatcgcatc
      601 aagtggtacg agtactgggt atcgcgccgg tcgtttgatt ttctattcta cagcgaacga
      661 tgtgatgcgt taccccgaag agggattggg aaatacctat atcttttcag ccaagaagac
      721 aggcatggga tatcgttatg aggtgattta tgacgacgag caacaccaag ccgatttctc
      781 gcgtccgccg ctggtcggcg agaaatgggt cgttcttaac atcaggcacc gggcaacagg
      841 aagttatgag gtactctact ccaaggtgga cgaatggact tggcattctc tcacctacag
      901 caaggtgtat gaagggaatg tggtggacaa ctatctcgcc ttcatcaacg ataaccgcga
      961 agtctatgtc tgtgacctcg acaaattgcc gtacaatccg gccaaggaat gtatcaggat
     1021 agatcgtgaa ggggaaaagg cctatcaacc acggttaaac gaagagaaca agaatcagtt
     1081 ggtctatttt gcgggagtcg gcgaatacgg gataactttt gttgatctgt cgaaccgcac
     1141 cccgcagtat acgcttctac cgataacacc atcagatcca caaccgttgg ggacatatcc
     1201 ccatcaattc aaaggcaact tgatattgta tagcgaactt ttcttgcccc caaacgagga
     1261 gatacgcgaa gatttcaaag cctgcttcta ccgtctcgat acgaagaaac agtattgccc
     1321 gaaaacccca gcttttgaag acggtcgcta cgatatgggc ttcaactctt ttgacggcaa
     1381 ctaccaactc tggaaagcac ccgccagcat caatgcccgt ttccgtgaca ttgcctgtta
     1441 ctgcgagaaa gaaggcatct gcccgttcga ggggatgacg gtcaagtcgc ccgttcctcg
     1501 accgacctta agatcgacca agcagacaaa gacaacgccg ccggaacctc tctttgcgcg
     1561 aacgaccgaa gatacctgtc gccgcatcat cgccaacgaa tcatgggaca aagagggcta
     1621 ctgcggtccc ctcggtgttg atgccatctt ccgctcctcg acctgtccgc cgctgtcggg
     1681 cgacccgaaa aaggcctttt ccgagtgtgt cgagtggcta tcgcggagca gggctttttc
     1741 tgaattgccc tgactttgtt acaaataaac gcaaaaattg ccctgacttt gttacgaaaa
     1801 aatgcaaaaa ttgccttgac tttattacaa aaacggccag aacaacctga tattattaaa
     1861 aaagattaat gtcgggtcga ggtgtgccgc ataagcaccg ttgaggagcc gtcgcggatg
     1921 acctcgtatc cgtccctctt gcctctctct cgtagcccct cggtgaccga gtaatgaaaa
     1981 acttttttcg tctttttttc ttccattttt tcttcttgcc tctttgcccg ttctggggta
     2041 tagtgccccg tgactttttt cccctaatta aaggaggaca ccatgacccg ctttttgatc
     2101 atcgccgccg ccgtcgccat cgccgtatcg tgcgccaccg aacagaagga ctactcgaag
     2161 gagcaggaaa aggcccgcga gcacggacag gaagcgttcc aggcccccga ggtcaacgaa
     2221 tagcaccgtc ccgcgccaac gcctcgaaag cgttggagat atcgagcgaa ccgaaagatt
     2281 ttcttgaccg gacggtctct gctcgtataa tctgccggtt tttttgagga accgcatgtt
     2341 cgacacgctg tcggaaaaac ttgagttgac cttccgccgc ctgcgcggcg tcggcaaact
     2401 ctccgaggac aatattcagg aggcggtgcg cgagatccgc ctgtcgctcc tcgacgccga
     2461 tgtcaactac ggcgtggtca agcagttcct cgaaaaggtg tcggcccgcg cggtcggcgc
     2521 cgaggtgacc cggtcggtga cccccggcca gcagttcgtg aaggtctgcc acgacgaaat
     2581 ggtggccctg ctcggcgccg gcgcccacga actcaacctc gcggtgcggc ctcccgccgt
     2641 catcctcatg gcgggcctgc aggggtcggg caagacgacg accgccgcga agatcggccg
     2701 ccacctcaag gaaaaactcc gcaaacgggt actgctcgtc cccgccgata tctaccgccc
     2761 cgccgccatc gaccagttga agaaactcgg cgagaccatc ggcattgatg tgttcgactc
     2821 gaagccgggc gacgacccgc tccgcatcgc cgaagcggcg atgaagcagg cgcgcgacgg
     2881 cgtctatgat gtcgtgatag tggacac
//
LOCUS       DAOQUE010000378         2898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_40396_length_2898_cov_4.502638, whole genome shotgun sequence.
ACCESSION   DAOQUE010000378 DAOQUE010000000
VERSION     DAOQUE010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40396_length_2898_cov_4.502638"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /gene="lysS"
                     /locus_tag="PKH10_10625"
     CDS             complement(<1..1255)
                     /gene="lysS"
                     /locus_tag="PKH10_10625"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNT28615.1"
                     /translation="MSQQHNPPAAPPLSENEVIRQKKEKVQALRSAGHHPYANTFRPD
                     TQVVAFRAACEAMPREELERPGKEVRYALAGRVMALRLMGKLAFGRLKDGSGAVQFVV
                     SKEYLGDKFDPFKKFVDIGDIIGLVGEPFVTRTGEVSILAHEVTLLTKNIQPLPEKFH
                     GLTDKEIRYRRRYLDLIMNDEVRETFLKRVRIINFIRDRMNRTGFLEVETPMMHPLVS
                     GAAARPFITHHNTLDMQLYLRIAPECYLKRLLVGGMEKVYEINRNFRNEGISIKHNPE
                     FTMMEFYWAYATYTDLMAFTEELVSSLATEVCGSTKIPYQGREIDLGAPWERLTIEES
                     LVKYGVSTADEAKSREGLLVAALRNGMDRDHVGPMSYYKLLVTLFEEKVEEHLWQPTF
                     ITRYPTELSPLARRNDKTPEWVDRFE"
     gene            complement(1252..1497)
                     /locus_tag="PKH10_10630"
     CDS             complement(1252..1497)
                     /locus_tag="PKH10_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28616.1"
                     /translation="MKKKLIVFGIIAVAVLAVVIGISLFSREDASSAPPVDRKQLRKT
                     LRIHPSQLKLNRELVNDALREKFKKEGIPPANEERPE"
     gene            1688..>2898
                     /locus_tag="PKH10_10635"
     CDS             1688..>2898
                     /locus_tag="PKH10_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28617.1"
                     /translation="MSRSLRLMFVSFAVLIPFIVCAADDFDDFDAEKSASDKEEKNQE
                     ESDQAIKDLLGGSEEEEVAPPKAEEKKASFDEEEDEDEDEEEAPKPEKVEKKESVDDI
                     LGTKKEEKKAAPAPTAQEIKPIVMVKGGINFGSYAKSFKQGLFFGSVDEGILGAEFVG
                     KSVFAKATINVRTQNPLLLSSNDKDNAIQYNPLMDDNYIENSIYKGIPYEAYGGMKLF
                     DMVTIRAGKMIPAYGILDRYQHLGVAIGTPFGTRSLLAIEGWIPETDAGFALGFNYDL
                     GDNSGINAEFMLGTGVIPNISQFWSTQKTMGMYFKGGYYSDMFTAMLGFQYRTDYEGT
                     AKKSVPFIGFGLGAIFSYAGFETWLTVDYTMAQLLKPKNSADPTLINGSTGGLSLSLM
                     PAYNLTIDYEM"
BASE COUNT          612 a          838 c          840 g          608 t
ORIGIN      
        1 gttcgaagcg gtcgacccat tcgggcgtct tgtcgttacg gcgggcgagc ggcgacagtt
       61 cggtcgggta gcgggtgatg aaggtcggtt gccacagatg ttcctcgacc ttctcctcga
      121 aaagcgtgac gagcagtttg tagtaactca tcgggccgac gtggtccctg tccatgccgt
      181 tgcgaagcgc ggcgacaagc agtccctcac ggcttttcgc ctcgtcggcg gtcgagacgc
      241 cgtatttcac gagactttcc tctatcgtca gccgttccca cggcgcgccg agatctattt
      301 cgcgtccctg atacgggatc ttcgtcgaac cgcacacctc ggtggcaagc gacgacacca
      361 gctcctcggt gaacgccatg aggtcggtgt aggtcgcgta ggcccagtag aactccatca
      421 tcgtgaactc ggggttgtgc tttatcgata tcccctcgtt gcggaagttg cgatttatct
      481 cgtatacctt ctccatgccg ccgacgagca ggcgtttgag atagcattcg ggggctatcc
      541 gcagatagag ttgcatgtcg agtgtattgt ggtgcgtgat gaacggccgc gccgcggcgc
      601 ccgacacgag cgggtgcatc atcggcgtct cgacctcgag gaacccggtc cgattcatgc
      661 ggtcgcggat gaagttgatg atgcgcaccc tcttcaggaa ggtctcacgc acctcgtcgt
      721 tcatgatgag gtcgagatag cgccggcggt aacggatctc cttgtcggtg agcccgtgga
      781 acttctcggg gagcggctgg atgttcttgg tgaggagcgt cacctcgtgc gcgaggatcg
      841 atacctcccc cgttcgggtg acgaacggct cgccgacaag cccgatgatg tcgccgatat
      901 cgacaaattt cttgaacggg tcgaacttat cgccgagata ttccttgctg accacgaact
      961 gcaccgcgcc ggagccgtcc ttgagccggc cgaaggcgag tttccccatg agccggagcg
     1021 ccatcacgcg gcccgcaagg gcatagcgca cctccttgcc cggtcgttcg agttcctccc
     1081 tcggcatcgc ttcgcaggcg gcgcggaagg cgacgacctg cgtgtcggga cggaaggtgt
     1141 tggcgtaggg atggtgtccc gccgaccgga gcgcctgcac cttttctttt ttttgccgga
     1201 tgacctcgtt ctcggaaagg ggcggggccg ccggaggatt atgctgctgg ctcattcggg
     1261 gcgctcttcg ttcgccggag gtatcccctc tttcttaaac ttctccctca acgcatcgtt
     1321 gaccagttca cgattgagtt tcagttggga ggggtgtatc ctcaaggtct tccggagttg
     1381 tttccggtcg accggcggag cggaagaagc atcctcccgc gagaacaagg atatcccgat
     1441 gacgaccgca agcacggcaa cggcgatgat gccgaaaaca atgagttttt tcttcatttt
     1501 tctcctcctt cgacgggaaa acgggacact atagtgtctt tcgggcgggg agtcaatttt
     1561 tcgcgccgca ccaccgtcac ctcctcttcg cgagaccgtc gcacaaaaaa acttgaattt
     1621 tacgtcacct ttccgtaagg tagaccgaca cttcactctt tgaaaggatt gtaaaggagg
     1681 ttccgtaatg tccagatcac tgaggttgat gttcgtttct ttcgccgttc tgatcccgtt
     1741 catcgtctgt gccgcggacg atttcgatga cttcgatgcc gaaaagtcgg cttccgacaa
     1801 agaggaaaag aaccaggaag aaagcgatca ggcgataaag gacctgctcg gcgggtctga
     1861 agaggaggag gtcgctcccc ccaaagcaga ggagaaaaag gcctcattcg atgaagaaga
     1921 ggacgaagac gaggacgagg aggaggcccc gaaaccggaa aaggtagaga agaaggaatc
     1981 ggtcgacgac atcctcggca ccaagaaaga agaaaagaag gcggcccccg ctcctaccgc
     2041 gcaagagatc aagccgatcg tcatggtgaa gggcggcatc aacttcggca gttacgcgaa
     2101 gagtttcaaa cagggtcttt tcttcgggtc ggtcgatgaa ggcatccttg gcgccgagtt
     2161 cgtcggcaaa tcggtgttcg cgaaggcgac catcaatgtc cggacccaga atccgctcct
     2221 tctcagctcc aacgacaagg acaacgccat ccagtacaac cccctcatgg acgacaacta
     2281 catagagaac agcatctaca aggggatccc ctacgaggcc tacggcggga tgaaactgtt
     2341 cgacatggtc accattcgcg ccggcaagat gataccggcc tacggcatac tcgatagata
     2401 ccagcacctc ggtgtcgcga tcggcactcc gttcggcacc cgctcgctcc tcgcgatcga
     2461 agggtggata cctgagaccg acgccggctt cgcgctcggg ttcaactacg acctcggtga
     2521 caattcgggc atcaacgccg aattcatgct gggtaccggc gtcatcccca atatatcgca
     2581 attctggtcg acccagaaga cgatgggcat gtacttcaag ggcggctatt actccgacat
     2641 gttcacggcg atgctcggct tccagtaccg gaccgactac gaagggaccg cgaagaagag
     2701 cgttccgttc atcggctttg gcctcggcgc catcttcagt tatgccgggt tcgagacatg
     2761 gctcaccgtc gattacacga tggcgcaact gctcaaaccc aagaacagcg ccgaccccac
     2821 actcatcaac ggttcgaccg gcggcctgtc gctttcgctc atgccggcct acaatctcac
     2881 gatagactac gagatgat
//
LOCUS       DAOQUE010000379         2894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_40485_length_2894_cov_3.346601, whole genome shotgun sequence.
ACCESSION   DAOQUE010000379 DAOQUE010000000
VERSION     DAOQUE010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40485_length_2894_cov_3.346601"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1894)
                     /locus_tag="PKH10_10640"
     CDS             complement(35..1894)
                     /locus_tag="PKH10_10640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT28618.1"
                     /translation="MNIAIVSFSPDRGDRHIDYHVHTTKAFVEERSGGRFMVDIVELS
                     MTDRARSAAGLLSGGRYEIILFHLSHWNVDYFREVAAALLEPKVLVGVWGHDSFSHPS
                     DYLKGVIKFIIQDEPELPLYEVAALVAEGRSPAEASDIIFRDDEQKTYRYSERRVLSP
                     LDLIPSPYLTGLIEVDDGTSVYWEVARGCLFRCDFCVEFSHLSPVRYHGFRYLEEELR
                     LFRDRGVSHLVIGAPIFNLNGQHFRKILGMIGEFLPEARVEMQVRPDLLSREEMDLLA
                     GMNVLLRFGIPTFKPKVLENLSTSLNVEKAVQNIRYINNYPDLSFTIDLMGGLPRTTY
                     QDLLADLEKIFTLSPVRIDLYRLSLYPGTRSYNRVREFELSMAHAYPWHVTAMPQFVK
                     RDIERIDEIAEGVELLYNRGRMVSVFTMLAAGLGISGTEVVERWGKYLRKSGASVTSE
                     SDFDILFGQLRAFFASLYDKLQKKKLWPLAEDLLMHNRLYTMSLMTPEEDRITFPYQM
                     EAMTADSIVGVNRSVFVHRFNYNIEDVVDAGYIDLKRFVSEEDKERLYGLIYRLEGGV
                     FARSVSDGEGTLFEHLAERGETTLGKLRRRFSKLDVDGIVSNWCEEGALYIAS"
     gene            complement(1930..>2894)
                     /locus_tag="PKH10_10645"
     CDS             complement(1930..>2894)
                     /locus_tag="PKH10_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28619.1"
                     /translation="LDAQLSKEQKQAFLAEHLAVSLAKFGDFKATDTLLRMVSKKFAL
                     KAAKEKKGEKKDKDAKIAEKMALRGQEVSIRMKGAEALAILGDPKAIPYLLEAIKAPE
                     DKEQNVEGQWIVFYEPKVWAADAYSRMISDPKAAEEFIVFANKFVAEARAYVKKVEDK
                     ERAALEKQMKGNKEFEAMKPEEKDKKIKAQAVLDPNYESAFRAATMMERFGKRVEIVK
                     ECAADPACYAKKLDDKEPAHAEKAVYMLGFAKKLSTHKEKVQPIFSHVEPYVRVALTQ
                     ALLKNEDKAFLPILAEAIKTEGDKVEYAESSKEYRAIYSYLQSL"
BASE COUNT          583 a          852 c          834 g          625 t
ORIGIN      
        1 gggacaccga gatacgcagg gaagatatct tcgactagga ggcgatatac agcgcccctt
       61 cttcgcacca attggacacg atgccgtcga catcgagttt cgaaaaacgc cggcgcagtt
      121 tgcccagcgt cgtttcaccc cgttcggcaa gatgttcgaa caacgttcct tcaccgtcac
      181 tcaccgagcg ggcgaagacg ccaccttcga gacggtatat caggccatac agccgttcct
      241 tgtcctcctc ggaaacgaac cgcttgagat cgatgtagcc ggcgtccacg acatcctcga
      301 tgttgtagtt gaaccggtgg acgaacactg atcggttcac cccgacgatc gagtcggcgg
      361 tcatcgcctc catctggtac gggaaggtta tccgatcttc ctcaggggtc atgagggaca
      421 tcgtgtagag gcggttatgc atcagaaggt cctcggcgag cggccagagt ttctttttct
      481 gcaacttgtc atacagcgac gcgaaaaagg cccgcaactg gccgaaaagg atgtcgaagt
      541 cgctctcgga ggtcaccgac gctccgctct tgcgcagata ttttccccaa cgctcaacga
      601 cctcggtgcc cgagataccg agccctgccg ccagcatggt gaagaccgac accatgcgcc
      661 cgcggttgta cagcagttcg accccttcgg cgatctcgtc gatccgttcg atatcacgct
      721 tcacgaactg cggcatcgcc gtcacgtgcc atgggtaggc atgcgccatc gatagttcga
      781 actcgcggac gcggttgtag ctgcgggtgc cgggatagag agaaaggcgg tagaggtcta
      841 tccggaccgg agagagcgtg aagatcttct caagatcggc gagcagatcc tgataggtcg
      901 ttcggggtaa accgcccatg agatctatgg tgaaggaaag atccggatag ttattgatgt
      961 atcggatatt ctggaccgcc ttctcgacgt tcagggaggt tgagagattc tcgagcacct
     1021 tcggcttgaa ggtggggata ccaaagcgga gcaacacatt catcccggcg agcaggtcca
     1081 tctcctcgcg cgagagaaga tctgggcgca cctgcatctc aacgcgcgcc tcgggcagga
     1141 actcgccgat catgccgagt atcttgcgga aatgctgccc gttcagattg aatatgggcg
     1201 caccgatgac gaggtgggac acaccgcggt ccctgaacag gcgcagttcc tcctcgagat
     1261 accggaaacc gtggtaccgc accggtgaga ggtgagagaa ctcgacgcag aaatcgcagc
     1321 ggaaaagaca cccgcgtgcg acctcccaat agaccgaggt accgtcatcg acctcgatga
     1381 gtccggtcag atagggcgac ggtatcaggt cgagcggcga caggacgcgg cgttcactgt
     1441 accggtatgt cttctgctcg tcgtcccgga agatgatgtc ggaggcctca gcggggctcc
     1501 tcccctcagc gacgagcgcg gccacctcgt acaacggcag ttccggctcg tcctgtatga
     1561 tgaatttgat cactcccttg agataatcgc tcggatgcga gaatgaatcg tgtccccaga
     1621 ccccgacgag aaccttgggc tcgagcagcg ccgccgccac ctcccgaaaa tagtcgacgt
     1681 tccaatggga aagatggaaa aggatgatct cgtaccgccc gccggaaagc agtcccgccg
     1741 cgcttcttgc gcggtcggtc atggagagtt ccacgatatc gaccatgaag cgaccaccgc
     1801 tccgttcttc gacgaaggct ttggtggtat gcacatgata atcgatgtgg cgatcacccc
     1861 ggtcagggct gaaggaaacg atggctatgt tcacgcggtc accccgggga tccgacggga
     1921 cgggtgtggt cagagcgact gaaggtaact gtagatggcg cggtattctt tggacgactc
     1981 ggcatattcg accttgtcac cttccgtttt tatcgcctcg gcaaggatgg ggaggaacgc
     2041 cttgtcctcg ttcttcaaga gcgcctgggt cagggcaacg cgcacatagg gctcgacatg
     2101 cgagaatatc ggctgcacct tttccttgtg cgtgctgagt ttcttggcga aaccgagcat
     2161 atagaccgct ttttcggcgt gagccggctc cttgtcgtcg agcttcttcg cgtaacacgc
     2221 gggatcggcg gcgcattcct tgacgatctc aacacgcttc ccgaaccgct ccatcatggt
     2281 agcggcgcgg aaggccgatt cgtagttcgg atcaagcacc gcctgcgcct tgatcttctt
     2341 gtccttctct tcgggtttca tcgcctcgaa ttccttgttc cctttcatct gtttctcgag
     2401 ggcggcgcgc tccttatcct cgaccttctt gacataggcg cgggcctcgg cgacgaactt
     2461 gttcgcgaag acgatgaact cctcggccgc cttgggatcg ctgatcatgc gggagtaggc
     2521 gtcggccgcc cacaccttcg gttcgtagaa cacgatccac tgaccttcga cattctgttc
     2581 cttgtcctcg ggcgccttga tcgcctcgag aaggtacggg atcgccttgg ggtcacccag
     2641 tatcgcgagc gcctcggcgc ccttcatgcg gatcgagacc tcctgaccgc gcagagccat
     2701 cttctcggcg atcttcgcat ccttgtcctt cttctcgcct ttcttctcct tggccgcttt
     2761 gagcgcgaac ttcttgctca ccatgcgaag cagggtatcg gtcgccttga aatcaccgaa
     2821 tttcgcgagc gagaccgcaa gatgctcggc gaggaaggcc tgtttctgct ctttggacaa
     2881 ctgggcatcg agtt
//
LOCUS       DAOQUE010000380         2893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_40510_length_2893_cov_3.209302, whole genome shotgun sequence.
ACCESSION   DAOQUE010000380 DAOQUE010000000
VERSION     DAOQUE010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40510_length_2893_cov_3.209302"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /gene="ruvB"
                     /locus_tag="PKH10_10650"
     CDS             complement(<1..648)
                     /gene="ruvB"
                     /locus_tag="PKH10_10650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006039849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNT28620.1"
                     /translation="MSDERIVETQFVPEDAAVEKRLRPQRLDQYVGQKNVVDNIVVYL
                     QAALKGNRALDHMLLAGPPGLGKTTLSLIMANELGVRITVTSGPALEKKGDLAGLLTS
                     LGERDILFIDEIHRLNAAVEESLYAAMEDFRFDIVIGEGPHARSMPLSLQPFTLIGAT
                     TKTGSLSSPLRDRFQITFRMDYYSDDELRIIVERSAAILGIPIERDGASEIARRSR"
     gene            complement(645..1256)
                     /gene="ruvA"
                     /locus_tag="PKH10_10655"
     CDS             complement(645..1256)
                     /gene="ruvA"
                     /locus_tag="PKH10_10655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNT28621.1"
                     /translation="MIGLVQGTVQFVDDRSCVLLTSGGVGYEIFATGTALAALTIGAP
                     ATFHVFTSVREDAITLYGFSSPDEKKLFLMLTDVDKVGPRIGLSILSSGTVPQIIGAI
                     MAGNAAFFSAISGIGKKTAEKILIDMRDKVHKIALSADVTPATGPDTTDRQAREAEMG
                     LIALGYHPSTVKAALAKITGKTTKRAEEIVREALGIIREQPKR"
     gene            complement(1261..1728)
                     /gene="ruvC"
                     /locus_tag="PKH10_10660"
     CDS             complement(1261..1728)
                     /gene="ruvC"
                     /locus_tag="PKH10_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNT28622.1"
                     /translation="MLILGIDPGSIKTGWGVIRKEGQRMIHVDNGLIMPPAGMAFKDR
                     VAFIFKGVLDTIDAFTPHHLSLEDIFMAKNAQSALKLGHVRGAVIAAAFLRGVPVAEY
                     TASHVKQAVTGNGRAEKFQIQEMVRVILGLREIAQEDASDALANALTHAFTLR"
     gene            complement(1753..2496)
                     /locus_tag="PKH10_10665"
     CDS             complement(1753..2496)
                     /locus_tag="PKH10_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNT28623.1"
                     /translation="MSGHSKWSTIKHKKGAADAKRGRMFTKLIKEITIAAKSGGGDPN
                     GNPRLRTAVQNARAASMPVDNITRAIKKGTGELEGEAFEEMTYEGYGPGGVAIMVDVT
                     TDNKNRSVSEIRRVFSKAGGNMGEAGCVGWMFEKKGQITIKKELVTEEKLMEIALDAG
                     AEDVVDSGDFWQVLTKPADLYAVNDSIQKAGLAIEESMLSRVPQNTIKVEGRVAEQVM
                     KLIEALEDNDDVSNVYDNSDIDPADMPKE"
     gene            complement(2525..2827)
                     /locus_tag="PKH10_10670"
                     /pseudo
     CDS             complement(2525..2827)
                     /locus_tag="PKH10_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          437 a          926 c          959 g          571 t
ORIGIN      
        1 acggctgcgg cgggcgatct ccgaggcgcc gtccctttcg atgggtatgc cgagtatcgc
       61 ggcgctccgt tcgacgatga tgcgtagttc gtcgtcggag tagtaatcca tacggaaggt
      121 tatctggaac cggtcgcgga gcggcgagga gagcgacccg gtcttggtgg tcgcgccgat
      181 gagcgtgaag ggctggagcg agagcggcat ggagcgcgcg tgcgggcctt cgccgatgac
      241 gatgtcgaac cggaaatcct ccatcgcggc gtagaggctt tcttcgaccg cggcgttgag
      301 ccggtgtatc tcgtcgatga aaagtatgtc gcgctcgccg aggctcgtga gcagtcccgc
      361 gaggtcgcct ttcttctcga gcgcgggacc gctcgtcacg gtgatgcgca ccccgagttc
      421 gttggccatg atgagcgaca gcgtcgtctt gccgagcccg ggggggccgg cgagcaacat
      481 gtggtcgagc gcgcggttgc ccttgagcgc cgcctgcagg tagaccacga tgttgtccac
      541 cacgttcttc tggccgacat actggtcgag ccgctggggg cgcagccgtt tctcgaccgc
      601 cgcgtcctcc ggcacgaact gtgtctcgac gatccgttcg tcgctcatcg cttcggttgc
      661 tccctgatga tgccgagcgc ctcgcgcacg atctcctcgg cccgtttcgt cgtcttgccc
      721 gttatcttgg cgagcgccgc cttgaccgtc gaggggtggt agccgagcgc gatgagcccc
      781 atctcggcct cgcgggcctg acggtcggtc gtatcgggac cggtggcggg cgtcacatcg
      841 gccgacaggg cgatcttgtg caccttgtcg cgcatgtcga tgagtatctt ttcggcggtc
      901 ttcttgccga tacccgatat ggcgctgaag aaggcggcgt tgcccgccat gatggcgccg
      961 atgatctgcg ggacggtgcc gctgctgagg atggagagcc cgatgcgcgg acccaccttg
     1021 tcgacatcgg tcagcatgag gaacagtttc ttctcatccg gcgacgagaa accatagagc
     1081 gtgatggcgt cctcgcgcac cgaggtgaag acatggaagg tcgccggcgc gccgatcgtc
     1141 agcgccgcga gcgcggtgcc ggtggcgaat atctcgtatc cgaccccgcc ggaggtcagc
     1201 aggacgcacg aacggtcgtc cacgaactgc acggtgccct gtaccagtcc tatcataggc
     1261 tcacctcagc gtaaaggcgt gggtaagggc gttggcgagc gcgtcggagg cgtcctcctg
     1321 cgctatctcg cgcaggccga ggatgacgcg caccatctcc tgtatctgga acttctcggc
     1381 gcgaccgttg ccggtgaccg cctgcttgac atgactcgcg gtgtactcgg cgaccgggac
     1441 gccgcgtagg aatgcggccg cgatcaccgc gccgcggaca tggccgagtt tgagcgccga
     1501 ctgggcgttc ttggccatga agatgtcctc gagactgagg tggtggggcg taaaggcgtc
     1561 gatggtgtcg agcactccct tgaatatgaa agcgacgcgg tccttgaacg ccatgccggc
     1621 gggaggcatg atgagcccgt tgtccacatg gatcatgcgc tggccctcct tgcggatgac
     1681 gccccatccg gtcttgatgc tgccgggatc gatgccgagt atgaggatac cgggaccggc
     1741 ggacacggga agctattcct tcggcatatc ggcgggatcg atgtccgaat tgtcgtacac
     1801 attggagaca tcgtcgttgt cctcgagcgc ctcgatgagt ttcatcacct gttcggcgac
     1861 ccgcccctcg accttgatgg tgttctgtgg cacccgcgaa agcatgctct cctcgatggc
     1921 gagccccgcc ttctgtatcg agtcgttgac cgcgtagaga tcggccggct tggtgagcac
     1981 ctgccagaaa tcacccgagt ccaccacatc ctcggcgccg gcgtcgagcg cgatctccat
     2041 cagtttctcc tcggtgacga gttccttctt gatggttatc tggccttttt tctcgaacat
     2101 ccagccgacg caccccgcct cgcccatgtt gccgcccgcc ttagaaaaga cgcggcggat
     2161 ctcggagacc gaccggttct tgttgtcggt ggtgacatcc accatgatgg cgacgccgcc
     2221 ggggccgtac ccttcgtagg tcatttcctc aaaggcctcg ccctcgagtt ctcccgttcc
     2281 cttcttgatg gcgcgcgtga tgttgtcgac cggcatgctg gcggcgcggg cgttctggac
     2341 cgccgtgcgc aggcgcgggt tgccgttcgg gtcgccgccg cccgatttcg cggcgatggt
     2401 gatctccttg atgagcttgg tgaacatccg cccgcgcttg gcgtccgcgg cgcccttctt
     2461 gtgcttgatc gtgctccact tcgagtggcc agacatggga cctcctgcgg ccgaggcggg
     2521 cttgctccgg gcggcccgcc gccagacacc acgagtgcag accgcccatc gagtgcccga
     2581 tcagcaccgc gggctcgccg atcgcggcca ccgcgtcgcc gaggtcggcg accatccgct
     2641 cggtgctgat cggatgcggg tcggcgacct cgcggccccg gtgccacggc gcgtcgtagg
     2701 tgtagacgct gcccagccgg gtcagccacg gcagctgacg cgaccaggtg ctgccgcgcc
     2761 ccatcagccc gtgcaccagc accagcggac gacccgcgcc gccgcgatgg gtcagctgcg
     2821 cgtccatgcg aacatcatgc cgatcggcgg ttcggtagcc tgatcggcat ggctgaccag
     2881 actcccgtgg tga
//
LOCUS       DAOQUE010000381         2850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_41430_length_2850_cov_4.774597, whole genome shotgun sequence.
ACCESSION   DAOQUE010000381 DAOQUE010000000
VERSION     DAOQUE010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41430_length_2850_cov_4.774597"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKH10_10675"
     CDS             <1..362
                     /locus_tag="PKH10_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28624.1"
                     /translation="NYLPEIPWGHGLGVYAGAEAILGNVVIDGHQHGLQVKGAKVFAG
                     ENCALPFGEEKGKTACVHILNNKTGINAFDLPADYDIFAAFANTNTWYEGNGTPFSGD
                     PEDVPTPPDPINNPTEN"
     gene            381..2819
                     /locus_tag="PKH10_10680"
     CDS             381..2819
                     /locus_tag="PKH10_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT28625.1"
                     /translation="MRTRGLFFAMMALLSLGACSEKSTTPNDEAATGDVDVAGDQLLS
                     DADTYDFWKDGPQRPHPVDWGTCPEGWEPKETKDEEGNLLAKYCEPPLNWRPAAPVDW
                     GTCPEGWEPMEEKDEDGNLLAKYCEPILPPDGTECEPGYFTMFGSVDCQRIGDECPDG
                     EFADIPTDAGTEIVYVFEGDSIQAAIDAAKDGAVIAIGKGTFDEFVTITDKNITLWGA
                     CVEGTILSPTTPMSDGAVDAIIRIDGTDPEKKVVVKNLTLTGGQRRGINLSKISADVL
                     NTAIMGTKRQGVAINGHGKLLLSSCIIKDTIASDDHTRGQGISIWNSDVNIIRGVFER
                     NKNSGIIALSDAGELSSITGTDLIVRNTQAQESDDLWGEGISTTGGVSFVGERVLLEN
                     NRDAGFFAATASSNDQGHLIAQYLIVRDTQPQKKDGTGGIGIMAQDTVTVEISKALLE
                     RNRTSGFYAVTREIAGSHGQVELTDIIVRDTIYQESDKNNGKGINFQDNISAKAERVL
                     LSDNREVGFFAGTFTEGAKGDIDIVDLVVIGTKEQESDKKFGRGVVVKDNMSALLARI
                     VVERNWDIGILASTSDDAEGTFVVSDVVVRDTGSQKSDGMMGRGISLTDNIAADLKRV
                     LSERSRHVGLLAGTSSSSNYAHFIGADLVVRDTLPQENDKSGGRGVAFQGNFKANISR
                     VLVKENREMGLGAGSFSVEDQGEIVATDIIVIDTEAQESDLRWGNGIAFQDVMATITN
                     ALVDNNREMGVYITRAEHETSEAYFTNVTISDTRVRECYEQDLPCNYLPEIPWGHGLG
                     VYAGDPAHNIHELS"
BASE COUNT          743 a          653 c          813 g          641 t
ORIGIN      
        1 gcaactacct accggagata ccttggggcc acgggctcgg agtgtatgcg ggagctgagg
       61 cgatattagg taatgtcgtc atcgatggcc atcagcatgg cctgcaggtg aaaggagcaa
      121 aagtgttcgc cggcgagaac tgtgccctgc ccttcggaga agaaaaaggc aagaccgcct
      181 gcgttcatat tctcaacaat aagaccggga tcaacgcgtt tgatctaccc gccgactacg
      241 acattttcgc cgcctttgcc aataccaaca cttggtacga aggtaatggc acaccttttt
      301 caggtgatcc cgaggatgtt ccgacgcctc cggatccgat caataacccg acggagaact
      361 aacaccaaga ggagaaaaac atgagaacga gagggctttt tttcgcgatg atggcgttgc
      421 tttcgcttgg tgcctgctcc gagaaaagca ccacgcctaa cgacgaggcg gccaccggcg
      481 atgtcgatgt tgctggcgac caactgctct ccgacgccga cacttacgat ttctggaagg
      541 acggtccgca acggcctcat ccagtggact ggggcacctg tcctgaagga tgggagccga
      601 aggaaacgaa agacgaggaa ggcaaccttc tcgccaagta ctgcgaaccg ccgttaaatt
      661 ggcgtcctgc tgctccggtg gattggggca cctgtcccga gggatgggag ccgatggagg
      721 aaaaggacga ggacggcaat ctccttgcca aatactgcga gccgatattg ccgccggacg
      781 ggaccgagtg cgagccgggt tacttcacga tgtttggctc agttgattgc cagcggatag
      841 gtgatgagtg ccctgacggc gagttcgccg acattccgac cgacgccggg acagaaatag
      901 tctatgtctt cgagggggat tccattcagg cggccattga tgccgcgaag gatggtgcgg
      961 tcatcgcgat aggtaaaggg acctttgacg agttcgtcac catcaccgac aagaacatca
     1021 ccctctgggg tgcctgtgta gagggaacga tcctttcacc gaccaccccg atgagcgacg
     1081 gagcggttga tgctatcatc cgcattgacg gcactgaccc ggaaaagaag gtcgtggtca
     1141 agaacctgac cctcaccggt gggcagaggc gcgggatcaa cctgtcaaag atctccgccg
     1201 atgtgttgaa tacagcgata atgggcacca agagacaggg agtagccatt aacggccacg
     1261 gaaagcttct tctttcttcc tgcattataa aagacacgat cgcttcggat gaccacacgc
     1321 ggggacaggg gatctctata tggaactctg atgtcaacat tattcgtggc gtttttgagc
     1381 ggaataagaa tagcggcatt attgctctct ctgatgcagg agaactgtcg agcataaccg
     1441 gcacggatct tatagtgaga aacacccagg ctcaggaaag cgatgatcta tggggagaag
     1501 gtatctctac cacgggaggc gtttcttttg ttggcgagag agtcttgttg gagaacaata
     1561 gagacgcagg ttttttcgct gcgacagctt cttctaatga tcaaggacat cttatcgctc
     1621 aatatcttat cgttcgtgac acgcaacctc aaaaaaaaga tggtactgga ggaatcggga
     1681 tcatggcaca ggatacggtc accgttgaga tatccaaagc gcttttggaa aggaacagaa
     1741 cctccggttt ttatgctgtc acgcgcgaga tagcggggtc gcacggacaa gtcgaactga
     1801 ccgacattat cgtgcgtgat acgatctatc aggaaagcga taaaaataat ggtaagggaa
     1861 taaattttca agacaatatt tccgcaaaag ccgaaagggt tctcttaagc gataacagag
     1921 aggtaggatt ttttgccggg acttttacgg aaggcgcaaa aggcgacata gatatcgtcg
     1981 atctggtggt gattggtacg aaagagcagg aaagtgacaa gaagttcggt cgaggtgttg
     2041 ttgtcaaaga caatatgagc gcactcttgg cgcgtatcgt tgttgagcgc aattgggata
     2101 ttggtatcct tgcatcaact tccgatgatg cggaaggaac tttcgtcgtt tctgatgttg
     2161 tggtgcgcga caccgggtcg caaaaaagtg acggcatgat gggccgaggg atcagtttaa
     2221 ctgataatat cgccgctgac cttaagaggg tgttatcgga aagaagcaga catgttggtc
     2281 ttcttgcggg gacatcatca tccagcaatt atgctcattt tattggcgcc gatcttgtcg
     2341 tgcgtgatac cctcccgcaa gagaatgata agtcgggagg caggggggtc gcttttcaag
     2401 ggaattttaa agccaatata tctcgtgttc ttgtaaaaga aaacagagaa atggggttgg
     2461 gcgccgggtc tttttctgtt gaggatcagg gggaaattgt tgcaaccgat attatcgtta
     2521 ttgacaccga ggcgcaggaa agcgatctgc gatggggcaa tggtatcgct ttccaagatg
     2581 ttatggcaac catcactaat gctttagtcg ataataaccg cgagatgggg gtatacataa
     2641 ctcgcgcaga acacgagaca agtgaggctt acttcaccaa tgtcaccatt tctgacacga
     2701 gagttcgtga atgttatgag caggatctcc cgtgcaacta cctaccggag ataccttggg
     2761 gccacgggct cggagtgtat gcgggagatc ctgctcataa cattcacgaa ctctcgtgaa
     2821 aaaaatggtg acattggaag taagcctcac
//
LOCUS       DAOQUE010000382         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_41694_length_2839_cov_3.620690, whole genome shotgun sequence.
ACCESSION   DAOQUE010000382 DAOQUE010000000
VERSION     DAOQUE010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41694_length_2839_cov_3.620690"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PKH10_10685"
     CDS             <1..1235
                     /locus_tag="PKH10_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNT28626.1"
                     /translation="FFRGERIIEILRKALESSKTLLTPGILFEGFGFQYVGPVDGHDL
                     TQLVETLEDLKPNEEPILLHVHTTKGKGFSPAEENPRSFHGVGPFDRRTGRPLKDVKA
                     GFTDHLGEYLPHLFRRDERLVAITAAMPDGTGLTRLQKEMPDRVYDVGMSEGHAVTFA
                     AGLAAAGRRPLVLIYSTFLQRAFDSIIHDVALQGLPVVFLIDRGGLVGEDGATHHGVF
                     DLSYLRIIPGLTVVAPRTETEMAGAIERAIRSDRPFAIRYPRGSSTGVSFSPQETPLP
                     EGCGELLLDAPGDTLVVTVGIGDQYAMSQRAVLNKEAIAYSVYDLRYVKPLPSELFDL
                     IRKRKIRKLVVIEENVVAGGAGSAILEELSRRRMQVRTELVGLPDEFVPHGSQKELRE
                     RYGVGLTALVEALRRVSR"
     gene            1232..2329
                     /gene="ispG"
                     /locus_tag="PKH10_10690"
     CDS             1232..2329
                     /gene="ispG"
                     /locus_tag="PKH10_10690"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNT28627.1"
                     /translation="MTRRVSREIRLGKSTIGGARPPLIQTMITTPLTRPEEAFAEADR
                     ALAMGCRIVRTAVRSETEIAGLATLAKRFGGDLIADIHFDHRLALAAIGCGVAGVRFN
                     PGNIGGKDHVTRIVEEARTRPDLALRIGVNAGSLEKDILEKHGGPTAAALVESTRRWV
                     EYLELELKYLNFKLSVKSHAVPETVAACQALAAFTDAPFHVGITEAGGGIGGRIRSAA
                     GIAILLHEGLVDTFRVSLTAPVEDEIVTGMYILKSCGLLDGGGEIVSCPTCGRTHGTL
                     FTYHDKLERFFDEHRWWLRPRLTVALMGCEVNGPGEAKEADLGIALGHGSALYFEKGS
                     LVRKFDDQDAAFVHLLRRIGEIWELAPVKGG"
     gene            2331..2711
                     /locus_tag="PKH10_10695"
     CDS             2331..2711
                     /locus_tag="PKH10_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNT28628.1"
                     /translation="MKKIVSTPDAPKAVGPYSQAVWAGDTLYISGQIPLDPATGAMVG
                     ATAAEQTAQVFRNIGAILKAAGIGFEHIVKMTVLLTDMKEFAAMNEVYARHFTGDFPA
                     RAAYQVCALPKGALVEIETVAYRG"
BASE COUNT          534 a          936 c          901 g          468 t
ORIGIN      
        1 ccttcttccg cggcgagcgg atcatcgaga tacttcgcaa ggcgctcgaa tcgtcaaaga
       61 cgttgctcac ccccggcatc ctgttcgagg gcttcggctt ccagtatgtc ggcccggtgg
      121 acggccacga tctcacgcaa ctcgtcgaga cactcgagga cctgaagccg aacgaggaac
      181 cgatactgct ccatgtccat accacgaagg gtaaagggtt ttcgcctgcc gaggagaacc
      241 cgcgcagttt ccacggcgtc ggtccgttcg accgccggac cggcaggccg ctcaaggatg
      301 tcaaggcggg cttcaccgac catctcggcg aatacctacc gcatctcttc cggcgcgatg
      361 agcggcttgt cgcgatcacc gcggcaatgc ccgacggtac cgggctcacc cgcctgcaga
      421 aagagatgcc cgaccgcgtc tacgatgtcg gcatgtcgga aggccacgcc gtcaccttcg
      481 cggcgggact cgcggcggcc ggtagacgcc cgctcgtcct gatctactcg accttcctcc
      541 agcgcgcctt cgactccatc atacacgatg tcgccctgca gggactgccg gtggtgttcc
      601 tgatagaccg cggcgggctt gtcggcgagg acggggcgac ccaccacggc gtattcgacc
      661 tctcgtacct gcgcatcatc cccggcctca cggtcgtcgc cccgcgtacc gagaccgaga
      721 tggcgggtgc catcgagcgg gcgatacgga gcgaccggcc cttcgcgata cgctacccgc
      781 gcggcagcag caccggtgtc tcgttctcgc cgcaggaaac gcctcttccc gaaggatgcg
      841 gcgaactctt gctcgacgcg ccgggcgaca cgctcgtcgt gaccgtcggc atcggcgacc
      901 agtacgcgat gtctcaacgc gcagtgctca acaaggaggc catcgcctac tcggtctacg
      961 acctccgcta tgtcaagccg ctcccgtcag aactgttcga ccttatcagg aaacgaaaga
     1021 tacggaaact cgtcgtcatc gaggagaatg tcgtcgcggg cggcgccggt agcgcgatac
     1081 tcgaggaact ctcccggcgc cggatgcagg tgcgcaccga actggtggga ctgcccgacg
     1141 agttcgtacc gcacggaagc cagaaggaac tgcgcgaacg gtacggcgtc gggctgactg
     1201 ctctggtcga ggcgctccgg agagtatcgc gatgacccga cgcgtgagcc gcgagatacg
     1261 gttggggaaa agcaccatcg gcggcgcccg accgccgctc atccagacga tgatcaccac
     1321 cccgctcacg cgccccgaag aggcgtttgc cgaggccgac cgggcgctcg ccatggggtg
     1381 ccgcatcgtt cgcaccgcag tgagaagcga gaccgagatc gcgggactcg ccacgctcgc
     1441 caagagattc ggcggcgacc tcatcgccga catccacttc gaccaccggc tcgcgctcgc
     1501 cgccatcggg tgcggcgtcg cgggggtccg tttcaatccc ggcaacatcg gcgggaagga
     1561 tcatgtgaca cggatcgtcg aggaggcacg cacacggccc gatctcgcgc tccgcatcgg
     1621 cgtcaacgcc ggatcgctcg aaaaggatat cctcgaaaag cacggcgggc cgaccgccgc
     1681 ggcgcttgtg gagagtacgc ggcgctgggt cgaatacctt gagctcgaac tcaaatacct
     1741 caacttcaaa ctgtcggtca aatcgcacgc cgttcccgaa acggtggcgg cctgccaggc
     1801 gctcgccgcg ttcaccgatg cgccattcca tgtcggcatc accgaggcgg gcggcggcat
     1861 cggcggccgc atccggtcgg cggccggcat cgcgatactg ctccacgaag ggctcgtcga
     1921 cacgttccgc gtcagcctca ccgcgccggt ggaggacgag atcgtgaccg ggatgtacat
     1981 cctcaagtcg tgcggcctgc tcgacggtgg cggcgaaata gtatcctgtc ccacctgcgg
     2041 tcggacccac gggacgctct ttacctacca cgacaaactc gaaaggttct tcgatgaaca
     2101 tcggtggtgg ctccgccccc ggctcaccgt cgcactcatg gggtgcgagg tgaacggccc
     2161 gggcgaagcg aaggaggccg acctcggcat cgcgctcggt cacggctcgg cgctctattt
     2221 cgagaaaggt tcgctcgtcc gcaaattcga cgatcaggac gccgccttcg tccatcttct
     2281 gcggcgcatc ggcgagatat gggaattagc accggtcaag ggaggatgag atgaaaaaga
     2341 tcgtttcgac gcccgacgcc ccgaaggcgg tcggacccta ttcgcaggcc gtgtgggccg
     2401 gcgatacgct ttacatctcg ggccagatac cgctcgatcc ggcgaccggc gccatggtcg
     2461 gcgcgacggc ggccgagcag acggcgcagg tcttccggaa catcggcgcg atactgaagg
     2521 cggcggggat cggttttgag catatcgtca aaatgacggt gctcctgacc gacatgaagg
     2581 agttcgcggc gatgaacgag gtctacgccc ggcacttcac cggcgacttc ccggcacggg
     2641 ccgcctatca ggtctgcgcc ctgccgaagg gcgctcttgt cgagatagag accgtcgcct
     2701 accggggctg acatgagata tcttgcgctg gcccttatcc tgacactcgg catcgtttca
     2761 tgcaaaaagg aggaggagaa cgccttcttc acgaagggga agaaggtgga gaccgggctc
     2821 atctgggaga agacgccgg
//
LOCUS       DAOQUE010000383         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_41800_length_2834_cov_4.661749, whole genome shotgun sequence.
ACCESSION   DAOQUE010000383 DAOQUE010000000
VERSION     DAOQUE010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41800_length_2834_cov_4.661749"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1456
                     /locus_tag="PKH10_10700"
     CDS             233..1456
                     /locus_tag="PKH10_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT28629.1"
                     /translation="MIISFSFENFFSFLERTEVSFLAGKQDPDTAHYTTTDTGLRLSK
                     LMSVVGANGSGKTNILRAYNFLMWLMRESFKLEPDAEIPLDQFLFTGDPRQETSFSLL
                     FELDGQVYRYELSLTKKQILNEIFSVKNGSNFRYLFKRTWDQKSTAYTVREQDFGKKV
                     EAIATGLLNKRNNSTLFSGLVFTNYEPLKPMVEYLASHKTNVSRLGAAAVIDTINPEM
                     VAALCVKYPEIRSSIESLMARIDLGISGIDIREIPLPEAVQKQLGIAKAYTPFGKHTV
                     KGAEYALHFRMESNGTQNLFVLLYQLLAALSRPGIAVMDEFEASLHPHMIPAILDLFV
                     SPETNPHNTQLVFTSHSAEVFNLLDKNQILLVEKDPETLVSEAWRLDDMNGVRRDDNL
                     YAKYMAGRYGAVPRL"
     gene            1468..2004
                     /locus_tag="PKH10_10705"
     CDS             1468..2004
                     /locus_tag="PKH10_10705"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB domain-containing protein"
                     /protein_id="HNT28630.1"
                     /translation="MSRTDPHKKKPRSPQRTLLIVCEGYTEHAFVRHLKETYFSRSNG
                     HIAVQNAYGGDPSSIIKKAEKILASQAFDRCLLVFDSDRIGKTKIPEKVCRTTVKSII
                     PRPCIENLFLRVLNSKSIPRTTDLCKKEFDTKHLKKHKKTEPATYAILFPETLLEKKR
                     GAITELDQLILIIQNCGW"
     gene            1994..>2834
                     /locus_tag="PKH10_10710"
     CDS             1994..>2834
                     /locus_tag="PKH10_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28631.1"
                     /translation="MDGERVTIARIVADKSLTDLTLAGKAAARFGLPIERLTKDELVA
                     EITALPLEERYQLAKRTLFFTTRKGPFLSACRCPSGADYRCCGYHTIQSVSGCPFDCS
                     YCILQHYIGNNPFISIFVNRESVEEELRIHLATHRHIRVGTGELADSLALDDLLDESG
                     FFLSMIARNGWEKRVTFEFKTKSDRVDTLISAHQRHPAVDCVAGFTLTLDRFHATDEI
                     GTVPVAKRLHAAARCAAAGVRVAFHFDPLVMQEQFLDDYRILAAQALGTVPHEKIAWI
                     SMGG"
BASE COUNT          680 a          847 c          708 g          599 t
ORIGIN      
        1 tttttctccg cttcctacaa gtatcgctgc tggcatcagt agttccattg tcactcccca
       61 agacaccgcc attgtgccgg atggcatcgc ctttgtcaag ccgatgcccg accaacaaga
      121 aaagacatga aaaatacaag aatcaacaga tttttgttgc ttttgctgtt tttcatgcta
      181 atatcgcctt gtgattgact gtcgccgacc atccgtcggc atcggaggaa ccatgatcat
      241 ttctttcagt ttcgagaact tcttttcctt ccttgaacgg acagaggtgt ctttcctcgc
      301 cgggaaacaa gaccccgaca ccgcccacta caccaccacc gacaccggcc ttcgcctatc
      361 aaaactgatg agcgtcgtcg gcgcgaacgg cagcggcaag accaacatcc tgcgcgccta
      421 caacttcctt atgtggctca tgcgcgaatc cttcaaactg gagcccgatg ccgaaatacc
      481 gctcgaccag ttccttttca ccggcgaccc gcgtcaggaa acatcgttct ctctcctctt
      541 tgaactggac ggacaggtct atcgctacga actctccctc accaagaagc agatactgaa
      601 cgagatattc tccgtcaaga acggaagtaa ctttcgctac ctattcaaac gcacttggga
      661 ccagaagagc actgcctaca cagtacggga acaggatttc ggcaaaaagg ttgaggcgat
      721 cgctaccggt ctgttgaaca aacgaaacaa ctccaccctc ttttccggcc ttgttttcac
      781 gaactacgaa ccgctcaaac cgatggttga ataccttgcc agccataaga ccaatgtgtc
      841 ccggctcggc gccgccgcgg tcatcgacac gataaacccc gaaatggtag ccgccctttg
      901 cgtgaagtat ccggagatac ggagttccat cgaatcgctc atggcgcgga tagacctcgg
      961 catcagcggc attgacatca gggagatacc gctgcccgaa gcggtccaga aacagttggg
     1021 gatcgccaag gcatacaccc cattcggcaa acacaccgtg aagggcgcgg agtatgcgtt
     1081 gcatttccgc atggaatcga acggcacgca gaatctgttc gtcctccttt accaactgct
     1141 ggccgctctc tcgcggccgg ggatcgcggt catggatgaa ttcgaggcga gtctgcaccc
     1201 gcacatgata ccggcaatac tcgatctgtt cgtttcgcca gagaccaatc cgcataatac
     1261 tcagttagtc tttacctcgc acagcgccga ggttttcaat ctgctcgaca agaatcagat
     1321 actgctcgtc gagaaagatc ccgagacatt ggtcagtgag gcatggcggc ttgatgacat
     1381 gaacggtgtc cgccgcgacg ataaccttta cgccaaatac atggcgggtc gctacggcgc
     1441 cgtcccgcga ctgtaaggag gcggacaatg tcacgcaccg accctcacaa gaaaaaaccg
     1501 cgctcaccac agaggacgct tctcattgta tgcgaaggat acaccgaaca tgctttcgtg
     1561 cgtcatctga aggaaacata tttctcccgc tcaaacggtc atattgccgt gcaaaatgct
     1621 tacggtggcg atccatcttc cataatcaaa aaggccgaaa agatccttgc atcgcaagcc
     1681 ttcgaccgtt gcttgctggt gttcgacagc gatcgtatcg gaaaaacaaa aatcccggag
     1741 aaagtttgtc gcaccaccgt gaagagcatt attccccgcc cttgcatcga gaatctcttt
     1801 cttcgcgttt taaacagcaa atccatccca agaacaacgg acctatgtaa gaaagaattt
     1861 gacacgaagc atctgaagaa gcacaaaaag accgagccgg cgacctacgc cattcttttc
     1921 cccgagaccc tgctggaaaa aaaacggggc gcgatcaccg aacttgatca gcttatcctc
     1981 atcatacaaa actgtggatg gtgaacgagt gaccatcgcc cgcattgttg ccgacaaaag
     2041 tcttaccgat ctgacgctgg ccgggaaggc ggcggcgcgt tttggtctgc cgatcgaacg
     2101 gttgaccaag gatgaactgg tcgccgagat aacggccctg ccgttggaag agcgatatca
     2161 actggcgaag cgaacgctct tcttcaccac ccgcaaagga ccgttcctgt cggcctgtcg
     2221 ctgtccctcg ggcgccgact accgctgctg cggctaccac accattcaga gcgtgtcggg
     2281 ctgcccattc gactgttcct actgcatcct acagcactac atcggcaaca atccgtttat
     2341 ctcgatattc gtgaatcgtg agtcggttga agaggagctg cggatccacc ttgcgacgca
     2401 ccgccatatc cgcgtcggca ccggcgaact ggccgacagc ctcgcgctcg acgatctctt
     2461 ggatgaatcg ggctttttcc tctcgatgat agcgcggaac ggctgggaaa agcgggtcac
     2521 ctttgaattc aagacaaagt cggaccgcgt cgatacgctg atctcagcgc accagcgaca
     2581 tccggccgtt gactgcgtcg ccggattcac cctgacgctc gaccgcttcc atgcgaccga
     2641 cgagatcggc accgtcccgg tggcaaagcg acttcacgcg gcggcccgtt gtgcggcggc
     2701 cggagtgcgg gtggcgttcc atttcgaccc gctggtgatg caggaacagt tcctcgacga
     2761 ctaccgtatc ctcgcggctc aggcgctggg cacggtgccg cacgaaaaga tagcgtggat
     2821 aagcatgggc ggcc
//
LOCUS       DAOQUE010000384         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_42050_length_2822_cov_4.247561, whole genome shotgun sequence.
ACCESSION   DAOQUE010000384 DAOQUE010000000
VERSION     DAOQUE010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42050_length_2822_cov_4.247561"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PKH10_10715"
     CDS             <1..560
                     /locus_tag="PKH10_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HNT28632.1"
                     /translation="LARENAGDDEMSRIAVLCAALMHDIGMTVGREDHEHTALLFAVP
                     ILDRLLKELYPKDLAKRVVIRSMAVEGIVGHMAHQRVHSLEAGVVLVADGLDMKKGRS
                     RIPMLISPGAKVGDIHQYSSASIEKVNIEKGSERPIRIGITMSESVGFFQVESVLITK
                     IEASSIKPYIELYAGKAGVPPRRYL"
     gene            576..2051
                     /gene="crtI"
                     /locus_tag="PKH10_10720"
     CDS             576..2051
                     /gene="crtI"
                     /locus_tag="PKH10_10720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNT28633.1"
                     /translation="MAGKKKVIVIGAGYGGLASAVLLAHKGFAVTLIEKNAVPGGRAR
                     LWEEKGYRFDMGPSWYLMPEAFDRFFDLIGKKREDYLPLDRLDPYYRVFFSKDEFVDI
                     GPDMKKTKKTFASFEKGGDEKLVKYLENCRIKYDTAVGEFLYRDYSSVLDFMNPTLLL
                     KGTKLSVFESLDSLVSKYFTDHRAKKILEYAMVFLGNSPYNAPAMYSMMSHLDLDLGV
                     FFPRNGMWGIVEALMELCREYGVEVKLGEPVRSIEMHGKDARAVVTDKGRYEADIVLA
                     NADYAHAETDLLPDAACGLDEGYWKKKTYAPSMFIMYLGVKKRLKRLAHHTLYFAADW
                     DHHFRQIFDDPAWPDQPSYYVSVTSKTQPDVAPKGCENVFVLVPVACNLDDNDAQRAA
                     YADKMIAHLETLAGENIRDHIEVKRIFSGRDFTADYNARYNTALGLSHTFDQTAVFRP
                     PRRSKKVKNLFYAGHYTHPGVGVPMVFIAAELAAKVIAGER"
     gene            2115..>2822
                     /locus_tag="PKH10_10725"
     CDS             2115..>2822
                     /locus_tag="PKH10_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HNT28634.1"
                     /translation="MKQLGTVLLSKQPFSEIVIGNTAIVRAMIESGTLVVTSYPGSPT
                     PEIAEAIKSIPTDQRPFYFEFSTNEKVATEVAYGASVNGHLSCVFFKSVGLNVAADTF
                     VQLGHMNLTGGMVIVWGDDPGAHSSQNEQDNRHFALLSYTPVLEPATPAEVYSFYKEA
                     ARISVEKGMPVVLRLTTHVCHQKEKVSFGGWDPKPWDKSPRFDPKNGPYIPITTEVFP
                     MKRRALKRLAELSAAFDA"
BASE COUNT          583 a          866 c          845 g          528 t
ORIGIN      
        1 atctcgcgag ggagaacgcg ggcgacgatg agatgagccg catcgcggtg ctgtgcgccg
       61 cgctcatgca cgatatcggc atgacggtcg gccgtgagga ccatgaacac accgcgctcc
      121 tgttcgccgt cccgatactc gaccgtctgc tgaaggaact gtaccccaaa gacctcgcca
      181 agcgggtcgt catccgttcg atggcggtcg aggggatcgt ggggcacatg gcgcaccagc
      241 gcgtccattc gctcgaggcg ggggtggtgc tcgtcgccga cgggctcgac atgaagaagg
      301 ggcgatcgcg catccccatg ctgataagcc ccggcgcgaa ggtgggggac attcaccagt
      361 acagttcggc gtcgatagag aaggtcaata tcgagaaggg gagtgagcgg ccgatacgca
      421 tcggtatcac gatgagcgaa tcggtgggtt tctttcaggt cgaatcggta ctcatcacca
      481 agatagaggc gagttcgata aagccctaca tagaactgta cgccggcaag gcgggtgtcc
      541 cgccccggcg ctacctgtaa agaaggagta tcgtcatggc gggaaaaaag aaggtcatcg
      601 tcatcggcgc cggttacggc gggctcgcgt cggcggtgtt gctcgcccac aaggggtttg
      661 cggtcacgct cattgagaag aatgccgtac cgggcggccg ggcgcggctc tgggaggaga
      721 agggctaccg gttcgatatg ggcccctcat ggtacctcat gcccgaggcg ttcgaccgct
      781 ttttcgatct gatcggcaaa aagcgcgagg actatctgcc gctcgaccgg ctcgacccgt
      841 attatcgcgt ctttttctcg aaggacgagt tcgtcgatat cggccccgac atgaaaaaga
      901 ccaagaagac cttcgcctcg ttcgagaagg gtggcgacga aaaactggtg aagtacctcg
      961 agaactgccg catcaagtac gataccgccg tcggcgagtt cctctaccgc gactattcgt
     1021 cggtactcga cttcatgaac ccgacgctcc tgctcaaggg gacgaaactc tcggtgttcg
     1081 agagtctcga ttcactcgtg tctaaatatt tcaccgacca ccgtgccaag aagatactcg
     1141 agtatgcgat ggtgttcctc ggcaacagcc cctacaatgc gccggcgatg tactcgatga
     1201 tgtcgcacct cgacctcgac ctcggcgtct tcttcccgcg caacgggatg tgggggatcg
     1261 tcgaggcgct catggaactg tgccgcgaat acggtgtcga ggtgaaactc ggcgaaccgg
     1321 tccgctccat cgagatgcat ggcaaggatg cgcgcgccgt cgttaccgac aaggggcgct
     1381 acgaggccga tatcgtgctc gcgaacgccg attacgccca cgccgagacc gatcttctgc
     1441 ccgatgccgc atgcggtctt gacgaagggt attggaaaaa aaagacctac gccccatcga
     1501 tgttcatcat gtacctcggc gtgaagaagc gactgaagcg gctggcccac catacgctct
     1561 atttcgccgc cgactgggat catcactttc gacagatatt cgacgatccg gcgtggcccg
     1621 atcagccctc gtactatgtc tcggtcacct cgaagaccca acccgatgtg gcaccgaaag
     1681 ggtgcgagaa tgtcttcgtg ctcgtgccgg tcgcctgtaa cctcgacgac aacgatgccc
     1741 agcgggccgc ctacgcggac aagatgatcg cgcacctcga aacgctcgcc ggcgagaaca
     1801 tccgcgacca catcgaggtg aagcgcatct tctcggggcg cgatttcacc gccgattaca
     1861 acgcccgcta caatacggca ctcggcctgt cgcatacctt cgatcagacg gcggtgttcc
     1921 gcccgccgcg ccggagcaag aaggtgaaga atctcttcta cgcgggtcac tacacccatc
     1981 ccggcgtcgg cgtcccgatg gtcttcatcg ccgccgaact cgccgcgaag gtcatcgccg
     2041 gcgaacggtg atttgacacc ttcctctttt tcggttagag tgctccgcaa agaacacctt
     2101 tccggaggtc cctcatgaag cagttgggaa cggtcctgct ctccaagcag cctttctccg
     2161 agatcgtcat cggcaacacc gccatcgtcc gcgccatgat cgagagcggc acgctggtgg
     2221 tgacgagcta tcccggctcg ccgacccccg agatcgccga ggcgatcaag agcatcccga
     2281 ccgaccagcg cccgttctat ttcgagttct ccaccaatga gaaggtggcg accgaggtgg
     2341 cctacggcgc gtcggtgaac ggccatctgt cgtgcgtctt tttcaagagc gtcgggctca
     2401 atgtcgccgc cgataccttc gtccagttgg ggcacatgaa cctcaccggc gggatggtca
     2461 tcgtctgggg cgacgacccg ggcgcgcaca gttcgcagaa cgaacaggac aaccgccact
     2521 tcgcgctcct ttcctacaca ccggtgcttg aaccggcgac gccggcggag gtctattcct
     2581 tttacaaaga ggcggcccgc atatcggtcg agaaggggat gccggtggtg ctccgtctga
     2641 cgacccatgt ctgccatcaa aaggaaaagg tgtcgttcgg cggatgggac ccgaagccgt
     2701 gggacaagag cccgcgattc gacccgaaga acggtcccta catacccatc acgaccgagg
     2761 tcttcccaat gaagcggcgt gccctcaagc gactggcgga actgtcggcc gccttcgacg
     2821 ca
//
LOCUS       DAOQUE010000385         2818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_42148_length_2818_cov_5.972856, whole genome shotgun sequence.
ACCESSION   DAOQUE010000385 DAOQUE010000000
VERSION     DAOQUE010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42148_length_2818_cov_5.972856"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(520..915)
                     /locus_tag="PKH10_10730"
     CDS             complement(520..915)
                     /locus_tag="PKH10_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28635.1"
                     /translation="MGWYGTYERTPRTSDGVKKHLIGEGYPIQEGELVGNTFYAAVVG
                     EREKSGLKDLRYAMVFLCEWGDGEFRYKPMDEFVNPYYYDCPKRIVDLLSPLSELPDL
                     KIEYQHDWRKKVYDRYGIDHRTLPLFAAN"
     gene            complement(1609..1959)
                     /locus_tag="PKH10_10735"
     CDS             complement(1609..1959)
                     /locus_tag="PKH10_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNT28636.1"
                     /translation="MRKDLNTITLSGRLGAAVNLHQKNGTEGPVKSASFSLACDRVEK
                     GEKKTDWFRVVLLGEQQVAACDKFLTKGSAVIVEGSLRVRSWETREGDTRKDVEIVAS
                     TVHFLDKRKEQKAA"
     gene            complement(2143..2598)
                     /locus_tag="PKH10_10740"
     CDS             complement(2143..2598)
                     /locus_tag="PKH10_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG family protein"
                     /protein_id="HNT28637.1"
                     /translation="MTIYRTQRGNDREERFVQTNIEEEGAGILGAPLSDIRVAVVGSR
                     TFFHYSVLEKELDTLLSKNATLLSGGAGGADALAEKYAREHGRTIEVIRPDWETYGPS
                     AGYRRNREIVKKASRVIAFWDGKSRGTDHIIQLARAEGIPVHVVRFASE"
BASE COUNT          462 a          860 c          845 g          651 t
ORIGIN      
        1 cgcaatactg tgttgctgcc tccctcgctc tcctcgactt acgagagtaa gccttgcgtg
       61 gctcggtcgt tgcgccgtgt cttgcgctcc gacccgagtc gcttgatacg aggagagagt
      121 gaaagggtcc tgtgttgtgt tattcgatcc gcccgccttc aaccgagggt catcaagagg
      181 ccatcagtac tcactcaaag gcgagcacct gtctgaacga agcgaatgcg aagagagttg
      241 cgcagcctcc tcttgatgat ccgatccggc tttgccggcg gttgtcccgg cgggcattag
      301 aaaaagatcg ttctttcccc cctttctgac gatacacaga aagggggtcg gcgaagagca
      361 tcgcgctcgc cgaaaccttc gccctcttcc cctagggctc atccgccgcc ccggccctcc
      421 gctggaggct tggggcctac gctacgggct atggcccaca ggccggcccg caggcggccc
      481 cagccgtgtc actggcggag gagggggcgg cgggccatct cagttggccg cgaacagcgg
      541 cagggtccgg tgatcgatcc cgtagcggtc atagaccttc ttccgccagt catgctgata
      601 ttcgatcttg aggtcaggca gttccgaaag gggcgagaga aggtccacta tccgcttggg
      661 acagtcgtag taatacgggt tgacgaattc gtccatcggc ttgtagcgga attccccgtc
      721 gccccattcg cagaggaaaa ccatcgcgta gcggaggtct ttgaggccgc ttttctcccg
      781 ttcgccgact acggcggcat agaaggtgtt cccgaccagt tccccttcct gtatggggta
      841 gccttccccg atgaggtgtt tcttgacccc gtcgctggtg cggggggtgc gttcgtaggt
      901 gccataccat cccattggtg tcctccattg gatgcgcgcc tcgtaatcgg gcgcaatact
      961 gtgttgctgc ctccctcgct ctcctcgact tacgagagta agcctgcgtc ggctcggtcg
     1021 ttgcgccgtg tcttgcgctc cgacccgagg cgcttgatgc gaggagaaaa agggaggggt
     1081 ccttgttgtt ttgtattaga tccgcccgcc ttcaaccgag ccgctgacca cggatagccg
     1141 caaggcgtcg gccgaggacc tgtttgaggc gccgaagcgt atcccgcgaa ggtggcgagt
     1201 tccgcaggcc ccgtggtcga ggcgatccgg caacgccggc ggttgtcccg gcgggcatta
     1261 accggtttct tctttcccct ctttcttctt tgccggacca aagaagaaag ggggtcggcg
     1321 aagagcatcg cactcgccga aatcttcgcc ttttcccctt catccgccgc cccggacctc
     1381 cgctggaggc ttggggccta cgctacgggc tatggcccac aggccggccc gcaggcggcc
     1441 ccagccgtgt cactggcagg gtctgccttt gcttaaaagc tatggcaaac ggaggagggg
     1501 ggcggcaccg gttacgcctc cggcgtcagc atttcgttct gaccgttctg tgtcttcacg
     1561 cgctctctgg attatcgtgc tttcgcgtcc gcacgggagg gaaggccgtt aggcggcctt
     1621 ctgttccttg cgtttgtcga ggaagtggac cgtggaggcc acgatctcga catccttccg
     1681 ggtgtcgccc tcgcgggttt cccacgagcg gacgcgcagc gatccttcga cgatgaccgc
     1741 cgaacccttg gtgaggaact tgtcgcacgc ggccacctgc tgttccccca gcaggacgac
     1801 gcggaaccag tcggtcttct tctcgccctt ctcgacgcga tcgcacgcga gcgagaacga
     1861 agcggacttc acggggcctt cggtcccgtt cttctgatgc aggttgacgg cggcgcccag
     1921 ccggccggaa agggtgatgg tgttcaggtc ttttctcatg atgttctcct tgagatgggt
     1981 cgaagagttc tcgatcgaat gccttcgttg tcgcgtactc gccttcctcg acataggtca
     2041 actatgcctt cgtcggctgt gccgcttcgt cttggccttc ttccgatagc tcttcgactg
     2101 tgttgttgtg tcccgaccgc gaccccgcga tcgggaaaaa tgttattcgg aagcgaaccg
     2161 gaccacatgg accggtatgc cttcggcgcg ggccagttga atgatatggt ccgttccgcg
     2221 ggacttcccg tcccagaagg cgatgacccg ggaagccttc ttgacgatct ccctgttgcg
     2281 ccggtacccg gcgctcggac cgtaggtctc ccagtcgggc cggatcacct cgatggtccg
     2341 gccgtgttct ctcgcgtatt tctcggcgag ggcgtcggcg cctccggcgc cgcccgagag
     2401 aagcgtagcg ttcttggaaa gaagcgtatc gagttctttt tccagcacgc tgtaatggaa
     2461 aaaggtacgg gagccgacca cggcgacccg gatatccgag agcggggctc cgaggatccc
     2521 cgcgccctct tcttcgatgt tcgtctggac gaaccgttct tcgcggtcat tgccgcgctg
     2581 tgtgcggtat atcgtcatgg tctttctcct tcagtaatat tcccgagctt cttcggcttc
     2641 ttcgtcagaa aggaagccgt tatcgctgag ccagtcagcg tgattgatta tgtcgccttc
     2701 gaaattcaca tgaatcttat ccataaaagc ctccaaaagc cccaacgggg gcgttgtgtt
     2761 gttgtgttaa agtgatactg tgatactgtc tttgcccgac tctcctgtgc cgtcctct
//
LOCUS       DAOQUE010000386         2810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_42314_length_2810_cov_4.217423, whole genome shotgun sequence.
ACCESSION   DAOQUE010000386 DAOQUE010000000
VERSION     DAOQUE010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42314_length_2810_cov_4.217423"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PKH10_10745"
     CDS             complement(<1..837)
                     /locus_tag="PKH10_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28638.1"
                     /translation="MMRRPFFFMLSALCLLSCDGGSGTPPADNDTVTVADEDGPTGDE
                     LLAGENEPPDAGFVPDVEAIASDYAGETGFIAIEPVTYYLRTERGTAGVKYLSDETRL
                     WYSFQPADEEWEKKPIALFFNGGPGSASGLLLAFNTGRRSMDPVFNGGDIVGDNPHRW
                     SSFANILYIDARNTGFSYSIVGDRPGFTTRNFNPFIDGADFVRLLLRVLAAHPALRGN
                     PVIFAGESYGGTRTIVMQNLLLNPARYAGNEGYFTDTALADEIAAHCAAVFPGEEVTP
                     EKA"
     gene            976..>2810
                     /locus_tag="PKH10_10750"
     CDS             976..>2810
                     /locus_tag="PKH10_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT28639.1"
                     /translation="MLHIAKKRELTEFFRSIPLEKKQQVYRGVRAVIAMLVKISKMTE
                     LYSIRDKIFTEFTETLMQNLDVIHNNIPCVSVTTKKIGFYYQERRINLFDAGERKFRN
                     IFLVNEIRELYFVKGITPEELLNFFSVIQETINYTLIDYDFNTRLWDRGVTHIGTLSD
                     PDMGDPEPWSAEEFKGESEPVAPAALCALTPRSFHDLAAIVKEFETVGAIGRSEFDAW
                     RRERGKAFTIQRYLEKARLLILRDGIEANRSIIGKMCEYALTNLQEGDFISGMVYLTN
                     LQQLRRSMEGADEALLGSVASVFEKIRSDQFINKVFEVAATIAPEHLPAFGEYLKIVS
                     AEELEPVFLKLVELGQKEARLHGLEAIAGNLTDDELVKRLATNPDWHVVRNLLYMLRF
                     SYRPSFLPTVREAMHHPTKQVRIEAAHVLSRYDADENVEFWHKAVLSPDEEVRVLAVE
                     NLLRIKGLVAKQILNEMFKPAQLVRFSTLDLEQYVGRILASKREEFFDLPGNLIFSDN
                     AQVRLMALKSLNTIEDPSAISSQVIRRIKSPEFETIEQHEMELLLALLKGTQLPSMLD
                     ALTPLLTMQGDLLNRKRYAPLKKRVFGFLRRRAAKSGTITKWLEKA"
BASE COUNT          627 a          834 c          821 g          528 t
ORIGIN      
        1 tgccttctcc ggcgtcacct cctccccggg gaagaccgcc gcgcaatgcg ccgcgatctc
       61 gtctgcaagc gcggtatcgg tgaaatagcc ctcgttcccg gcatagcgcg cggggttcag
      121 caggagattc tgcatcacga tggtgcgggt gccgccgtac gattcgcccg cgaagatgac
      181 cggattgccc cgcagcgcgg gatgggccgc gaggacacgc aggagcagac ggacgaagtc
      241 ggcgccgtcg atgaacggat tgaaattacg ggtcgtgaac ccgggccggt cgccgacgat
      301 gctgtaggag aagccggtat tgcgggcatc gatgtagagt atgttggcga aggaactcca
      361 gcgatgcgga ttgtcaccga cgatatcgcc gccattgaag acggggtcca tgctccggcg
      421 accggtattg aaggcgagca ggagccccga cgcagagccg ggaccgccgt tgaaaaagag
      481 tgcgatcggc tttttctccc actcctcgtc ggccggctgg aatgaatacc acaaccgcgt
      541 ttcgtcgctc aggtatttta cgccggcggt gccgcgttcg gtccgcagat agtaggtcac
      601 cggctcgatg gcgatgaagc cggtctcgcc ggcataatcg ctcgctattg cttcgacatc
      661 ggggacgaat cccgcgtcgg gcggctcgtt ttcgccggca agcaattcgt cgccggttgg
      721 accatcctcg tcagcgacag tgaccgtgtc gttatccgcg ggcggcgttc cggaaccacc
      781 atcacacgac agcagacaca gcgccgacag catgaaaaag aaaggtcttc gcatcatccg
      841 gagcctccag tatcgtctcg tagatagata atgtagccga acgggaagcc aaagcaaaag
      901 aaatgggaaa aaactttgct tgctatactc tcttgggcta tagttggtgt accgtatgtt
      961 tgagcggggc gaccggtgct ccatatcgcg aaaaaacggg agttgaccga attcttccgg
     1021 tcgatacccc ttgaaaagaa acagcaggtc taccgcggtg tccgcgcggt gatcgccatg
     1081 ctcgtgaaga tatcgaaaat gaccgagttg tactctatcc gcgacaagat cttcaccgaa
     1141 ttcacggaaa cgctgatgca gaatctcgat gtgatccaca acaacatccc atgtgtctcg
     1201 gtcaccacca aaaagatcgg attctactat caggagcggc gtatcaatct gttcgatgcg
     1261 ggggaacgga agttccggaa catcttcctc gtcaacgaga tacgtgaact ttatttcgtc
     1321 aagggcatca cccccgagga actcctcaac ttcttctcgg tcatacagga gacgataaac
     1381 tacaccttga tagattacga tttcaacacc cgcctctggg accgaggtgt gacacatatc
     1441 ggcaccctgt cggaccccga catgggcgac cccgaaccgt ggagcgctga agaattcaaa
     1501 ggggagtccg agcccgtggc gcccgccgcg ctgtgcgccc tgacgcctcg gagtttccac
     1561 gacctcgccg ccatcgtcaa ggagttcgag acggtcggcg cgataggtcg cagcgaattc
     1621 gacgcctggc gcagggagcg cgggaaggcc tttaccatcc agcggtacct tgaaaaggcg
     1681 cgactcctca tcctgcgcga cggtatcgag gcgaaccggt cgatcatcgg caagatgtgc
     1741 gaatacgccc tgaccaatct gcaggagggc gatttcatct cggggatggt ctacctgacc
     1801 aacctgcaac agttgcgacg gtcgatggag ggggccgacg aggcgcttct cggatcggtc
     1861 gcctccgtgt tcgagaagat acgatcggat cagttcatca acaaggtctt cgaggtcgcg
     1921 gcgaccatcg cgccggaaca ccttcccgcc ttcggagaat atctgaagat cgtcagcgcc
     1981 gaggaacttg aaccggtctt cctcaaactg gtcgaactcg gccagaaaga ggcgcgcctc
     2041 cacggactcg aggcgatcgc gggcaacctc accgacgatg aactcgtcaa gcggctcgcg
     2101 acgaatcccg actggcatgt cgtccgtaat cttctgtata tgctcaggtt ctcttaccgt
     2161 ccctccttcc tgccgaccgt ccgggaggca atgcaccacc cgaccaaaca ggtgcgtatc
     2221 gaggcggcgc atgtgctctc gagatacgac gccgacgaga atgtcgagtt ctggcacaag
     2281 gcggtgctct cccccgacga ggaggtgcga gtgctcgcgg tcgagaacct gctccgcatc
     2341 aaaggattgg tggcgaaaca gatactcaac gagatgttca agccggccca actcgtccgg
     2401 ttcagcacgc tcgatctcga acaatatgtc ggccggatac tcgcatcgaa acgagaggag
     2461 ttcttcgacc tgccgggcaa tctcatattc agcgacaacg ctcaggtgcg gctcatggcg
     2521 ctcaagagcc tcaacactat cgaggatccc tccgcgatat cgagtcaggt catccgacgg
     2581 atcaaaagcc ccgagttcga gacgatcgaa cagcacgaga tggaactgct cctcgccctt
     2641 ctcaagggga cgcagttgcc gagcatgctc gacgccctga cgccgctctt gaccatgcaa
     2701 ggggacctgc tcaaccgcaa gaggtacgcg ccgctcaaaa agagggtgtt cggcttcctg
     2761 cgccgccggg ccgccaagtc gggcaccata acaaagtggc ttgaaaaggc
//
LOCUS       DAOQUE010000387         2801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_42526_length_2801_cov_5.320102, whole genome shotgun sequence.
ACCESSION   DAOQUE010000387 DAOQUE010000000
VERSION     DAOQUE010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42526_length_2801_cov_5.320102"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PKH10_10755"
     CDS             complement(<1..481)
                     /locus_tag="PKH10_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HNT28640.1"
                     /translation="MSEIETTAPQSKLIAFESRKIRRIWHNEEWFFVVEDVVRALIDS
                     KDAKQYIKRMKLRDPELAKGWVQFVPTLPVMTPGGVQNMACANTEGLFRIIQTIPSPK
                     AEPFKRWLAKVGHERVQEIEDPEKAAQRMRELYKAKGYPEEWIERRMRGIAIREELTD
                     "
     gene            complement(546..1424)
                     /locus_tag="PKH10_10760"
     CDS             complement(546..1424)
                     /locus_tag="PKH10_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28641.1"
                     /translation="MEKKKMMHNKNQHPAEIYNSIRRIVFSIYHRDGYDHAKEILEKN
                     RSKLNVQSMVGLKAELSFYHRFQKEYCLTPALDSGEHVDFAGNIGIRPVRFDVTTNEK
                     FKEFKNYEKFLCEGYDYRIAIMDGKNWEIKDVIDLAFPKCPSCGNGNLFSIAVLLGEN
                     FNHKGESQWTHDQLLLDVCPYCEEFRERDSTTTHFLPKISDYAQEVDHEDLLEDDYKR
                     IVEGHTHNAFDYLRKLWEIKTIKLMGLADSTYFTTSKDGDGYWGIKFQLLQDVVRNRF
                     PDHIQTGMLENTSEEW"
     gene            complement(1440..2030)
                     /locus_tag="PKH10_10765"
     CDS             complement(1440..2030)
                     /locus_tag="PKH10_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28642.1"
                     /translation="MNKKKGTQQSISEVVVVVLIILGLTVVLSVFLVFILSVQKEIAI
                     TVIAGVLTVSGSVLSLLITKQHDKQLEIMRDLRNKKTPVYIGFVDSLFSAMYSFKEGH
                     KAMTQEEMALNFEKYTKELMIWGSDEVVNSYKEFHSKLKTPPQTDADRLAVLDNFGSL
                     ILSIRKDLGYQNRDIAPRDLLSLFISDLHKIEAANK"
     gene            complement(2027..>2801)
                     /locus_tag="PKH10_10770"
     CDS             complement(2027..>2801)
                     /locus_tag="PKH10_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HNT28643.1"
                     /translation="SRFHELPGNVWNILITEKNAEFLKRLTTSFAQLGNIAKINRGLI
                     TGDRDSYFATKQATSKHVPILAGGDIYRYHFSQPSEFVLFERPKTAGGCWDREVHLAP
                     HKIAVRQIGEGPTATWINKPIAVTGNIFTVRGTDKEHELFLLGILNSKLVDYFWRVMF
                     ADFKGSFPQVTIFSLEQVPVREIDQKKKSDREAHGKMVKLVEQTLSLHEQAATARTPQ
                     EKTANDRQISATDAQIDRLVYDLYGLTEEEIKIVEGSML"
BASE COUNT          652 a          677 c          610 g          862 t
ORIGIN      
        1 cgtcggtcaa ctcctcgcgg atggcgatgc cgcgcatgcg ccgctctatc cactcttcgg
       61 gatagccctt cgccttgtac agttcgcgca tccgttgggc ggccttttcg gggtcttcta
      121 tttcctgcac ccgttcgtgg ccgaccttgg ccagccaccg tttgaagggc tcggccttcg
      181 gcgatgggat ggtctgtatg atgcgaaata gcccttcggt gttggcgcag gccatgttct
      241 gaacgccgcc cggggtcatg acgggaaggg taggtacaaa ctgtacccat cctttcgcaa
      301 gttccgggtc gcgcagtttc atccgtttga tgtactgttt ggcatctttt gaatcgataa
      361 gggcccgtac cacatcctcg acgacgaaga accattcttc gttatgccag atgcgccgta
      421 ttttgcggct ttcaaaggcg atgagtttgc tctgcggggc ggtcgtctcg atctcgctca
      481 tgattgctcc tcaacgatta tgatctgttc attaccttac ggtcgggcat gaatcaaagt
      541 ctccgctacc actcttcgct tgtgttttct aacatgcctg tttggatatg atcaggaaat
      601 ctgtttctaa ctacatcttg gagaagttgg aacttaatac cccaatatcc atcgccatcc
      661 ttggatgttg taaagtatgt gctgtctgcc aaccccatta acttgatcgt ttttatttcc
      721 cataatttcc tcagataatc aaaggcgtta tgggtatgac cctctactat tcttttataa
      781 tcgtcttcga gcagatcttc gtgatctact tcttgcgcat agtcagatat cttgggtaag
      841 aaatgtgtgg tcgtagagtc cctttctcta aactcttcac aataaggaca gacatctaac
      901 aataattgat catgcgtcca ttgagattcc cccttatgat tgaaattctc tcctagaagc
      961 acagctattg aaaacaagtt cccgttacca catgagggac atttgggaaa ggccaagtca
     1021 atcacatcct taatttccca attctttccg tccataatgg ctatacggta gtcatatccc
     1081 tcgcagagaa atttctcgta attcttaaat tctttgaact tttcatttgt ggtaacatca
     1141 aatctcacag gccttattcc gatatttcca gcaaagtcaa catgttcccc tgagtctagg
     1201 gcgggggtca aacaatactc cttttggaac ctatgataaa aagaaagttc tgcttttagg
     1261 ccaaccatgc tctgaacatt caattttgat ctgttctttt ctagtatttc tttcgcatga
     1321 tcatatccgt ctcgatggta tattgaaaac actattcgtc ttatgctatt atatatctct
     1381 gctggatgtt gattcttgtt gtgcatcatt ttcttttttt ccatttcctg acctctattt
     1441 tatttgttgg cggcttcaat tttatgaaga tcgcttatga aaagagatag caaatcacga
     1501 ggggcaatgt ccctgttctg gtaaccgagg tcctttctta ttgacagaat tagggagcca
     1561 aaattatcga gcactgctag ccgatcggcg tctgtttgcg gaggtgtctt cagtttagaa
     1621 tggaactcct tataagagtt aacaacttca tctgatcccc atatcatcaa ttcttttgtg
     1681 tatttctcaa aattgagcgc catctcttct tgagtcatgg ccttatggcc ttccttgaat
     1741 gaatacatgg cgctgaaaag agagtcgaca aaacctatat agaccggcgt ttttttgttt
     1801 cttaagtctc gcatgatttc cagttgcttg tcatgctgtt tggtaatcaa taacgataat
     1861 actgaacctg aaactgtcaa tacaccggca attacagtaa ttgctatttc tttctgaacg
     1921 ctcagaataa agactaagaa aacactgagc actacggtaa gccctaaaat aatcaagaca
     1981 accactacaa cttcagaaat gctttgttgg gttcctttct tcttgttcac aacatactcc
     2041 cttccactat cttaatctcc tcttccgtca aaccgtagag gtcatagacg aggcggtcga
     2101 tctgggcgtc ggtggcggat atctggcggt cgttggcggt tttctcttgc ggggtgcggg
     2161 cggtggcggc ctgctcgtgg agcgaaagcg tctgttcaac gagtttcacc attttgccat
     2221 gcgcttcgcg gtcgctcttt ttcttctggt cgatctcgcg caccggcacc tgctccaaac
     2281 taaagatagt cacctgcggg aaggaacctt tgaagtccgc gaacatgacc cgccagaagt
     2341 aatcgacgag ctttgagtta agtatcccta acaggaaaag ttcatgctct ttgtccgtgc
     2401 ctcgaaccgt aaaaatgttt ccggtcacgg ctattggctt gttgatccat gtcgcggtcg
     2461 gtccttctcc aatctgtcgc actgcgattt tatgcggtgc cagatgaacc tcgcgatccc
     2521 aacaaccgcc tgccgtcttg ggacgctcaa ataacacgaa ttccgacggt tgagaaaaat
     2581 gataacggta gatgtctcca ccggcaagaa taggaacatg ctttgatgtg gcctgttttg
     2641 ttgcaaagta gctgtcacga tcgcccgtta taagtccccg gtttattttt gcaatgttgc
     2701 cgagttgggc aaaagaggtg gtcaaccgct tgagaaattc ggcattcttc tctgtgataa
     2761 gaatgttcca gacattcccc ggtagttcgt gaaaccggct t
//
LOCUS       DAOQUE010000388         2791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_42753_length_2791_cov_5.145102, whole genome shotgun sequence.
ACCESSION   DAOQUE010000388 DAOQUE010000000
VERSION     DAOQUE010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42753_length_2791_cov_5.145102"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PKH10_10775"
     CDS             <1..923
                     /locus_tag="PKH10_10775"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNT28644.1"
                     /translation="LYGDSAASLVPSSDLLDRFDTVLYDVQDIGSRYYTYLQSLSLFM
                     DELSIRPKRLIVLDRLNPIGGDIVEGSSIQERYYSFVGRFLLPHRHGLTTGEVARYYY
                     LLKGYSFPFEIVPIEGWRRDSFADEYDLPWVPTSPNMPTLETAILYPGGCLVEGTNLS
                     EGRGTAFPFRVVGAPGIDPFKLADELSTLGLPGLRFLPLEFRPMFQKEAMKRCGGVYI
                     DLADRRAVRPLRAYIAILAVFRGLLGDDGFFRVKPYEFVTDIPAIDLLLGDTCLIDLF
                     YKRAPLDKIDGYLAAAEENWLIARRPFLLY"
     gene            1044..2222
                     /locus_tag="PKH10_10780"
     CDS             1044..2222
                     /locus_tag="PKH10_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28645.1"
                     /translation="MSLLAGFFLNPLFLLAVVAFLGSRLLRHRSAALKLILFNALTIL
                     CLFALFDLLAAVLLLPEQARFRTMDRFTHHGLQPLIDRETKWGDISTPYRVRTNDLGM
                     VDATMRTVPKKSDKKRILLLGDSFVEGVGLPWEDTFAGMLSADLAPRGVEVLNAGVAS
                     YSPKLYYLRMQQVLAAGLQVDEVFLFLDIGDIQDEVVYDYFVPDKEPVDPLMNRAMIF
                     FSHHSWIYRSWHERFFTRQENPKYEYGPYWGGIKGYHRVRARWMYDTTEFERWGKEGL
                     EKATLHTEKLLALLKEHGIKVTLSIHPWPEHVRQRISHSMHETHWKIFALDHAVELID
                     LFPLFINERPAEEVIAEYFIAGDIHWSRAGHRLVADKVLERIGDKRTMNREVVHVAGR
                     "
     gene            2206..>2791
                     /locus_tag="PKH10_10785"
     CDS             2206..>2791
                     /locus_tag="PKH10_10785"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNT28646.1"
                     /translation="MLPVGRAEDARRMDRAAIKKGTPSLLLMENAAFSLFLAVNELLS
                     SFRPDRIVVFAGKGGNGGDGMAFLRILADRGCPIPLMLVPFFDPSDLSGDTLANYRML
                     PRSVTRMKNYRQLNGRILFVDALLGTGLSSSLDLEILHAVDFINEYKAKRVVSVDVPT
                     GLSSDTGAMFPRAVHADMTISFGILKRGLFLDQGP"
BASE COUNT          553 a          813 c          751 g          674 t
ORIGIN      
        1 cgctctacgg cgattctgcc gcgagtcttg tcccctcttc cgatctgctc gaccggttcg
       61 acaccgtcct ctatgatgta caggacatcg gaagtcgcta ctacacctat ctgcagagtc
      121 tttccctctt catggacgaa ctctcgatac gccccaagcg attgatcgtg ctcgaccgcc
      181 tgaaccccat cggcggcgat atagtcgaag gaagctccat tcaagagcgc tattacagtt
      241 ttgtcggccg ttttcttctt ccccatcggc acggattgac gaccggcgag gtagcgcgct
      301 attactacct gcttaaaggg tactcgttcc ccttcgagat tgtcccgata gaaggctggc
      361 gacgcgacag ttttgccgac gagtacgatc ttccatgggt gccgacctcc cccaatatgc
      421 cgacactcga gaccgcgata ctctatccgg gcggctgtct tgtcgagggg accaaccttt
      481 cggaaggccg cggcaccgcc ttcccgttca gggtcgtcgg tgcgccgggt atcgatccgt
      541 tcaaactggc cgacgaactc tccacgctgg gtctgccggg acttcggttc ctgccgctgg
      601 agtttcgccc gatgttccag aaagaggcga tgaaacggtg cggcggtgtc tatatcgatc
      661 ttgccgaccg gcgtgccgtt cgaccgcttc gcgcctacat cgcgatcctc gccgtttttc
      721 ggggcctgct cggcgatgat ggttttttcc gcgtaaaacc ttacgagttc gtcaccgata
      781 tcccggcgat agatctcctg ctcggcgaca cctgtctcat agacctgttc tacaagcggg
      841 cgcctctcga caagatcgac ggctatctgg cggccgccga agagaactgg ttgatcgccc
      901 gacgcccctt tctcctgtac tgagcggacc ccggaagatg aaaaatctcg gacattcttg
      961 aaaaatcgaa agaaatatgt gcaatggtcc tgtccgttac ctgaggaaat gatgcgtacc
     1021 gaccctgtaa cgagaggctc ttaatgtcgc ttctggccgg tttttttctt aatcctcttt
     1081 ttctgctggc ggtcgtcgcc tttctcggtt cgcgtctcct ccggcatcga tcggcagccc
     1141 tgaaattgat cctcttcaac gcgctgacca tcctctgcct tttcgcgttg ttcgatctgc
     1201 tcgccgcagt gcttctgctc ccggaacaag cccggttccg caccatggac cgtttcacgc
     1261 atcacggtct tcagccgctg atcgacaggg aaacgaaatg gggggacatc tcgacgccct
     1321 atcgagtgcg gaccaacgat ctcgggatgg tcgatgcgac gatgcgcacc gttcccaaaa
     1381 aaagcgataa aaaacgaatc ctgcttctcg gcgattcctt tgtggagggt gtgggactcc
     1441 cgtgggaaga cacctttgcc ggcatgctct cggccgatct ggccccgcgc ggcgttgagg
     1501 tgctgaacgc cggtgtcgcc tcctattcgc cgaaacttta ctatcttcgt atgcaacagg
     1561 tgctcgccgc cggccttcag gtcgacgagg tgttcctctt tcttgatatc ggggacattc
     1621 aggatgaggt cgtgtacgac tacttcgttc ccgacaagga acccgtcgat ccgctcatga
     1681 accgggcgat gatcttcttt tctcatcatt catggatcta ccgctcgtgg catgagcgat
     1741 tcttcacccg tcaggagaac ccgaaatatg agtatggccc ttactggggc gggatcaagg
     1801 gataccatcg ggtacgcgct cgatggatgt acgacacgac ggaattcgaa cgctggggca
     1861 aagaggggct tgaaaaggcg acgcttcata ccgagaaact cttggccctg ctgaaggaac
     1921 atggtatcaa ggtgaccctt tcgatacatc cgtggcccga gcatgtacgg caacggatat
     1981 cgcactcgat gcacgaaaca cattggaaga tcttcgcgtt ggatcacgca gtcgaactta
     2041 tcgacctttt tcctcttttc atcaacgaac gaccggccga agaggtgata gccgaatatt
     2101 tcatagcggg cgacatacat tggagccgcg caggacatcg tctggtcgcc gacaaggtgt
     2161 tggaacggat aggggacaag cgaaccatga acagagaggt ggtccatgtt gccggtcggt
     2221 agagccgaag atgcgcgccg gatggaccgt gccgcgatca agaaaggtac cccgtcgctt
     2281 cttctgatgg agaacgccgc cttctccctg ttccttgcgg tgaacgagct tctttcttcc
     2341 tttcgccccg accggatcgt cgtgttcgcc ggtaagggag gcaacggcgg tgacggcatg
     2401 gcgttcctac ggatacttgc cgaccgtggt tgtcctatcc cattgatgtt agtgccattt
     2461 tttgacccca gtgatctgtc gggagatacc ctcgccaact atcggatgct tcctcgctcg
     2521 gtgacccgca tgaagaacta ccggcaactg aacggtagga tcctctttgt cgacgctctg
     2581 ctgggcaccg ggctttcttc ttctcttgat ctagaaattc ttcatgccgt tgatttcatt
     2641 aatgaatata aggctaaaag ggtcgtctcg gtcgatgttc cgaccggtct ttcaagcgat
     2701 accggcgcca tgttcccccg ggcggtacac gctgacatga cgatcagttt cggcatactt
     2761 aaaagaggtc tcttcctcga tcaaggaccg a
//
LOCUS       DAOQUE010000389         2789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_42812_length_2789_cov_4.649232, whole genome shotgun sequence.
ACCESSION   DAOQUE010000389 DAOQUE010000000
VERSION     DAOQUE010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42812_length_2789_cov_4.649232"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKH10_10790"
     CDS             <1..248
                     /locus_tag="PKH10_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28647.1"
                     /translation="GADLLSQRPFPDFRPDALIGDTKEIVVQVNGKVRDKIVVPTTAT
                     KEEVEAAVRAKDFSKFLNSGSIKKIIYVPDKLLNVVG"
     gene            248..1288
                     /gene="holA"
                     /locus_tag="PKH10_10795"
     CDS             248..1288
                     /gene="holA"
                     /locus_tag="PKH10_10795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNT28648.1"
                     /translation="MAAKTSFPPKATELIDFKKDGGVRVFFSTERFLIRCLKQSAEEA
                     GRAAGHTVASYDGTEPSQLREAISASREIGFFATRKIVLLDFFIQGKEKLDAATLTLL
                     ESAIAKPEQDNLLLLFFNDLDKRTKFYKAVTGKGYKQFFHELSAPEKTTVTAFIVSQF
                     GPVKPDQRLINHFLQKESGDLFHIETEVGKLRLWAESAGKRSLTLADAEPMLSSFGEE
                     KIFKIMDHLVKGDKVRAIELYRDLKTGEGEQKVHPVLISLLMKHFRVILDGRILQSKK
                     RSGEIPALLQENNAFYLKYSIAEVLARYKNRTVIAALNDAAKLELGMKGVYEVAMPDV
                     SSSIEQFMLKYF"
     gene            complement(1329..2759)
                     /locus_tag="PKH10_10800"
     CDS             complement(1329..2759)
                     /locus_tag="PKH10_10800"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNT28649.1"
                     /translation="MQNILDRVTKRGVQFADVRLTVTDSESFSFERGSLKDYGLTQDN
                     VSIGIRVLVGGVFGFAGVNDLSESSIDAAIAAAFRNAEESRRFRRSAIEFPAQRPVVA
                     SRVIAPKEDPFAMSYGDKIGWFMPIVQGLTGGKRTIYSRFDAEFRRQYKIYANTEGTL
                     VETTRYHVNPEMSVLASDGNAVMSRTWPGMMSSRAGGFEVMRDFAFPEHIDTIIAEAD
                     QLLDAPRITEERADLIIGGSQLALQLHESVGHATEADRIFGMEISYAGKTFVKPEHIG
                     KLRYGSPQMTVVADSTDETGIGFNLTDDDGVPAKRATIIKEGILTDLQTSRETAALLG
                     LEPSSNMLAVHGSDIPLIRMNNLNLMPGKAGSLQDLIADTEKGYLVDLTKTWSIDDSR
                     NNFQFTTEIGWKIEKGKITGIVKEPTYYGITTEFWNSCDAVCSESEWKYWGTFFCGKG
                     EPGQAMQLSHKTAPARFRNVVVNVKA"
BASE COUNT          555 a          862 c          811 g          561 t
ORIGIN      
        1 gcggcgccga cctcctctcg caacgacctt tccccgactt ccggcccgat gcgctcatcg
       61 gcgacactaa ggagatcgtc gtgcaggtca acggcaaggt gcgcgacaag atcgtcgtcc
      121 cgaccactgc taccaaggag gaggtcgaag cggccgtacg cgcgaaggat ttctcgaaat
      181 tccttaactc cggctccatc aagaagatca tctatgtccc cgacaaactg ctcaatgtgg
      241 tcggctgatg gccgctaaga catcctttcc gcccaaggcg accgaactca tcgacttcaa
      301 gaaggacggc ggggtacggg tattcttttc gaccgaacgg ttcctcatcc ggtgcctcaa
      361 acagagcgcc gaggaggcgg gtcgggcggc cggacacacc gtcgcctctt acgacggcac
      421 cgaaccatca caactacgcg aggcgataag cgcttcccgc gagatcggct ttttcgcgac
      481 acgcaagatc gtcctgctcg atttctttat tcaggggaaa gagaaactgg acgccgcgac
      541 gctcacgctc ctcgaatcag ccatcgcgaa gccggagcag gacaaccttc tcctgctctt
      601 cttcaacgat ctggacaagc ggacaaaatt ctataaagcc gtcaccggca agggatacaa
      661 gcagttcttc cacgaactct ccgcccccga aaagacgacc gtcaccgctt tcatcgtctc
      721 tcagttcggg ccggtgaaac cagaccagcg gctcatcaac cactttctgc agaaggaaag
      781 cggcgatctt tttcacatcg agaccgaggt gggcaaactg cggctctggg ccgaaagcgc
      841 cgggaaaagg tcgctgacgc tcgccgacgc ggaaccgatg ctctcctcct tcggcgagga
      901 aaagatattc aagataatgg accatctcgt gaagggcgac aaggtccgcg ccatcgaact
      961 ctaccgcgac ctgaagaccg gcgagggcga gcagaaggtg caccccgtcc tcatctcgct
     1021 cctcatgaaa catttccggg tgatactcga cggccgtatc ctgcagagca agaaaaggtc
     1081 cggcgagata ccggcgctcc tgcaggagaa caacgcgttc tatctgaagt acagtatcgc
     1141 tgaggtcctc gcccgttaca agaaccgcac cgtcatcgcg gcgctcaacg atgcggcgaa
     1201 actcgaactc ggcatgaagg gggtctatga ggtggcgatg cccgatgtca gttcatctat
     1261 tgaacaattc atgttgaagt atttctgaaa ccttcgtccc ccttgtcaaa ggggggcctt
     1321 ggggggattt acgccttcac attcaccacg acattgcgga agcgggcggg ggcggtcttg
     1381 tgggagagtt gcatcgcctg tcccggctcc cctttgccgc agaagaaggt gccccaatac
     1441 ttccattcgc tctcggaaca gacggcgtcg cacgaattcc agaactcggt ggtgatgccg
     1501 tagtaggtcg gctccttgac gatgccggtt atcttcccct tctctatctt ccagccgatc
     1561 tcggtggtga actggaaatt gttgcggctg tcgtctatcg accaggtctt ggtgagatcg
     1621 accagatacc ccttctcggt gtcggcgatg agatcctgca agctccccgc cttccccggc
     1681 atcaggttga ggttgttcat ccggatgagc gggatgtcgg aaccgtggac cgcgagcatg
     1741 ttggaactcg gttcgagccc gagcagcgcc gcggtctcgc gcgatgtctg gaggtcggtg
     1801 agtatccctt cctttataat ggtcgcgcgc ttggccggga cgccgtcgtc gtcggtgagg
     1861 ttgaagccga tgccggtctc atcggtcgaa tcggccacca ccgtcatctg cggcgaaccg
     1921 tagcgcagtt tgccgatatg ctccggtttc acgaaggtct tcccggcgta actgatctcc
     1981 atcccgaaga tacggtcggc ctcggtggcg tgccccaccg attcgtgcag ttggagcgcc
     2041 aattgcgagc cgccgatgat gagatcggcc cgctcttcgg ttatccgcgg ggcgtcgagc
     2101 agttggtcgg cctcggcgat gatcgtatcg atatgctcgg ggaaggcgaa gtcgcgcatc
     2161 acctcgaacc cgccggcgcg cgacgacatc atcccgggcc aggtccgcga catgacggcg
     2221 ttgccgtcgg aggcgagcac cgacatctcg gggttgacat ggtagcgggt cgtttcgacg
     2281 agcgtccctt cagtgttcgc gtatatcttg tactggcggc ggaattcggc atcgaaccgg
     2341 ctgtagatgg tccgcttgcc gccggtgagc ccctgcacga tgggcatgaa ccagccgatc
     2401 ttgtcgccgt aggacatcgc gaaggggtct tctttcggcg caatgacgcg gctcgcgacc
     2461 accggtctct gcgccgggaa ttctatcgcg ctgcggcgga accggcgcga ctcctccgca
     2521 ttgcggaacg cggccgcgat ggcggcgtcg atgctactct ccgacaggtc gttcaccccg
     2581 gcgaacccga acaccccgcc gaccagcacc cggatgccga tggagacatt atcctgcgtg
     2641 aggccgtagt ccttgagact gccgcgctcg aacgaaaaac tctcgctgtc ggtgaccgtc
     2701 agccgcacat cggcgaactg caccccgcgc ttcgtgaccc ggtcgagtat gttctgcact
     2761 cttttttcat gtcactctcc ggcaaaaaa
//
LOCUS       DAOQUE010000390         2785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_42897_length_2785_cov_4.543223, whole genome shotgun sequence.
ACCESSION   DAOQUE010000390 DAOQUE010000000
VERSION     DAOQUE010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42897_length_2785_cov_4.543223"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PKH10_10805"
     CDS             <1..502
                     /locus_tag="PKH10_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNT28650.1"
                     /translation="YVPADDLTDPAPATTFAHLDATTVLSRELAAMGIYPAVDPLDST
                     SRILTPDVVGDDHYAVARQVQTILQRYKDLQDIIAILGMDELSEEDKTVVYRARKIQR
                     FLSQPFSVAEAFTGMPGKFVKREDTIKGFRMLVNGEMDRYPEAAFLMVGGIEEAVEKG
                     EKLLKG"
     gene            505..924
                     /gene="atpC"
                     /locus_tag="PKH10_10810"
     CDS             505..924
                     /gene="atpC"
                     /locus_tag="PKH10_10810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNT28651.1"
                     /translation="MALHVHILTHTKTLYDEDGGAVLLPATEGQMEVLPRHIPFVVLL
                     DTGVVAVKRDDRELFECTVSSGIAYLSNDDLTVFADAAETIDTIDLARANAAVDEARD
                     WIERHRADPIMHQAGLKYLRRAENRIRFVQERRKIKK"
     gene            982..2253
                     /locus_tag="PKH10_10815"
     CDS             982..2253
                     /locus_tag="PKH10_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNT28652.1"
                     /translation="MKRFLTPLFLCAALSFVIACGGDDPKEDPAVTLKYQAADDHAAA
                     VSPEVVAANNDFAARIFKAMATAEPGKNLMISPLSIEIALAMTMNGASGGNLAEMRTV
                     LGFDDQTLAAIDAQFLHLIKSLESADKDMVLAIANSVWMDELFEPRLKSAFKEALLNS
                     FEAEPYTVDMADTATADLINQWVSDNTSGKIEKIIDDINADTVMFLINALYFKAAWTV
                     TFDEDRTYDGQFELTDGTDKTVKMMTFKDAQDFKFFSSGYGAAGGYSAVRLPYGRDKF
                     AFYGFVPNDGTVDEFIVRMADEGLDGFFSNLTEKKEVPVLLPKFKFAYEKSLNDILKG
                     LGMEQLFVPGGLMNMAEPGDGLYVSEVKHKTYIEVNEQGTEAAAVTSVEVGDTAIPEG
                     FYATKPFVFVIRDDRSGTILFMGKVEDPSQS"
     gene            2338..>2785
                     /locus_tag="PKH10_10820"
     CDS             2338..>2785
                     /locus_tag="PKH10_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28653.1"
                     /translation="MREIIALLILSLTVVSCENFSENMFVPDGAAAVDTAPLADADTV
                     VSDEAVGDELPIMDDDAPFHDEDVLLVDEPAPGDEDYAPVEVDELPESDILPAPAVVW
                     IRVWGSERVDSGHSVEVDAAGDIYVAGIAGTNMDDQTGPGAICSYKT"
BASE COUNT          569 a          834 c          866 g          516 t
ORIGIN      
        1 ctacgttcct gccgacgacc tgaccgaccc ggcgcccgcc accaccttcg cccacctcga
       61 tgcgacgacg gtgctttcgc gcgagctcgc cgcgatgggc atctacccgg cggtcgaccc
      121 gctcgattcg acctcgcgca tcctgacgcc cgatgtggtc ggggacgatc attacgcggt
      181 ggcgcggcag gtgcagacca tactccagcg ctacaaggac ctgcaggaca tcatcgcgat
      241 actcggcatg gacgaactgt cggaggagga caagacggtg gtgtaccggg cccgcaagat
      301 acagcggttc ctttcccagc cgttcagtgt cgccgaggcc ttcaccggca tgcccggcaa
      361 gttcgtgaag agggaggata ccatcaaggg cttccggatg ctcgtgaacg gcgagatgga
      421 tcgctacccc gaggcggcgt tcctgatggt cggcggcatc gaagaggcgg tggagaaagg
      481 ggaaaaactg ctgaagggct gaccatggcg ttgcatgtgc acatactgac ccataccaag
      541 acgctctacg acgaggatgg cggggcggtg ctcctgccgg cgaccgaggg acagatggag
      601 gtcctgccgc gccacatacc gttcgtcgtc ctgctcgaca ccggcgttgt cgccgtgaag
      661 cgcgacgatc gcgaactgtt cgagtgcacc gtgtcgtcgg gtatcgccta tctttcgaac
      721 gatgacctca cggtgttcgc cgacgccgcc gaaacgatcg acacgataga tctcgcccgg
      781 gcgaacgccg cggtggatga ggcccgtgac tggatagagc ggcaccgcgc cgacccgatc
      841 atgcatcagg cggggctcaa gtacctgagg cgcgccgaga atcgcatccg cttcgtgcag
      901 gaacggcgga aaataaaaaa ataaatatcc cccgcgcccg gtaccaaagg ggcgttgacc
      961 gttttttaaa tggagaaagc catgaaacgc ttcctgaccc cgttgtttct ctgcgcggcg
     1021 ctttcgttcg ttatcgcctg cggtggcgac gacccgaagg aggaccccgc ggtcacactc
     1081 aagtatcagg cggccgatga ccatgcggcg gcggtgagcc ccgaggtggt cgccgccaac
     1141 aacgacttcg cggcacgcat cttcaaagcg atggcgaccg ccgagccggg gaagaacctc
     1201 atgatatcgc cgctgtccat cgagatagcg ctcgcgatga cgatgaacgg cgcctcgggc
     1261 ggcaacctcg ccgagatgcg gacggtgctc ggcttcgacg accagacgct tgccgcgata
     1321 gacgcgcagt tcctccacct catcaagagc cttgaatcgg ccgacaagga catggtgctt
     1381 gccatcgcca actcggtctg gatggacgaa ctgttcgagc cgcgtctcaa gtcggcgttc
     1441 aaggaggcgc tcctcaactc gttcgaggcg gagccctaca cggtcgatat ggctgacacc
     1501 gctaccgcgg acctcatcaa ccagtgggtg agcgacaaca cgagcggcaa gatagagaag
     1561 atcatcgacg acatcaacgc cgacacagtc atgttcctca tcaacgcact ctacttcaag
     1621 gcggcgtgga cggtgacctt cgacgaggat cgtacctacg acggccagtt cgaactgacc
     1681 gacggcaccg acaagacggt caagatgatg acctttaagg atgcgcagga tttcaaattc
     1741 ttctccagcg gctacggcgc cgcgggcggg tacagcgccg tgcggcttcc ctacggtcgc
     1801 gacaagttcg ccttctacgg ttttgttccg aacgacggga cggtggatga gttcatcgtc
     1861 cggatggccg acgaggggct cgacgggttc ttctccaacc ttaccgagaa gaaagaggtc
     1921 cccgtgttgc ttccgaagtt caagttcgcc tacgagaagt cgctcaacga catcctcaag
     1981 ggcctcggca tggaacaact cttcgtgccg ggcggcctga tgaacatggc ggaaccgggc
     2041 gacgggctct atgtctccga ggtgaagcac aagacctaca tcgaggtgaa cgagcagggg
     2101 accgaggcgg cggcggtgac atcggtcgag gtgggcgata cggcgatccc cgaagggttc
     2161 tacgccacca agccgttcgt attcgttatc cgcgacgatc gctccggcac gatcctcttc
     2221 atggggaagg tcgaggatcc ctcgcagtcg taataaacaa atcccctgcc gcaacttttt
     2281 acttgcaagg accgtcagtc ttcctagact gacgatatcc gataatggag tttgttcatg
     2341 cgggagatca tcgcactgct tatcctgtcg ctgacggtcg tgtcctgcga gaatttttcc
     2401 gaaaatatgt ttgttcccga cggcgccgct gcggtcgata cggcgcctct cgccgatgcc
     2461 gatacggtgg tgtccgatga ggcggtcggc gatgaactgc cgatcatgga cgatgatgcg
     2521 ccgttccacg acgaggatgt cttacttgtc gatgaacctg cgccgggtga cgaagactat
     2581 gcgcccgtcg aggtcgacga acttcccgag agcgacatcc tgcccgcccc ggcggtcgtt
     2641 tggatccggg tatggggttc ggagcgggtc gacagcgggc attcggtgga ggtcgatgcg
     2701 gcgggcgata tctatgtcgc cggtatcgcg ggcaccaata tggacgatca gacaggtccc
     2761 ggcgcgatct gtagttacaa gacct
//
LOCUS       DAOQUE010000391         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_43295_length_2767_cov_4.793879, whole genome shotgun sequence.
ACCESSION   DAOQUE010000391 DAOQUE010000000
VERSION     DAOQUE010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43295_length_2767_cov_4.793879"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKH10_10825"
     CDS             <1..298
                     /locus_tag="PKH10_10825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT28654.1"
                     /translation="VMLQHLRKADVAFRYAGDEFVVFLPYTPKRNAQVVVERLWENIR
                     KHDFVIDGASFRIAASYGWAGFPEDGRTAKEVIAKADEAMYRAKRRSRDSFEMA"
     gene            300..860
                     /locus_tag="PKH10_10830"
     CDS             300..860
                     /locus_tag="PKH10_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNT28655.1"
                     /translation="MIARYLDTFPRIDGSAVIFRSATIIGDVTIGPEANIWFGAIARG
                     DVNHIRIGARTNVQDNAVIHVTAAKYPTEIGDEVTIAHHTVIHGSIIGDRTLIGMGAV
                     LLDGCRVGSEVMVAAGSLLRTGTVIPDGVLVAGNPATVKRPLTEAERLFLKRSADNYV
                     KYSRNYLTSSPDTLYSLEELKNGAQW"
     gene            893..2458
                     /locus_tag="PKH10_10835"
     CDS             893..2458
                     /locus_tag="PKH10_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNT28656.1"
                     /translation="MARSLVFSVILFLTVLVSAQEKETMLTVLFMNDIHGMAWQYDES
                     DNPGIGGLAAVKTLVDQIRAEVQGKGGDVLALMAGDIALGDPRSNVCKNLPVVKGMNL
                     VGFDATVVGNHDLFFGIDTFNEMKKESKFDWISANIYKEGGTKPLADREYIEKTFDSG
                     LKVAILGLSTREIEQITTAGLSGDIVVTDPIQEAKTRVPLLKKNNHLVIALTHLGYFD
                     TDKSYDGFEGDNFLARSVPGIDLIVGGHTHRQFYAPVKIGDSLIIQTEGMGKWLGRFD
                     LFVKGGKITKNYFKMYPINLKKKVGSGNNVSYQFMEKEIKENKAMLDMLAGFKCEFSE
                     TLLGTIETPLAGYREEARFKEIELGDIIADIMRERGKTDIAFFNAGSIRQGLPAGKIT
                     ERELYSMFPFLDTLFVGKIKGKELQDVLDYFAQKGQGAGGFLQVSGLSMKIYKGSALD
                     IKIGGKPLDKNKVYTLAINSFIASGGDGYTMLKDLKNKKDTFISLPAILVEYLKKNKK
                     FPKPEMGRLIIVQ"
     gene            2512..>2767
                     /locus_tag="PKH10_10840"
     CDS             2512..>2767
                     /locus_tag="PKH10_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNT28657.1"
                     /translation="MTTVVGIGNALVDILVTLPDDSLLHRLDLPRGTMNLVDRDRSRA
                     VLDATGRLSRTVSSGGSAANTVHGLTRLGVPCSFVGKVSDD"
BASE COUNT          642 a          839 c          774 g          512 t
ORIGIN      
        1 ggtgatgctc cagcatctcc gcaaggccga tgtggcgttc cgctacgccg gcgacgaatt
       61 cgtcgtgttc ctcccctaca ctcccaaacg gaacgcacag gtggtcgtcg aacgcctctg
      121 ggagaacatc cggaaacacg attttgtgat agacggcgca tccttcagga tcgccgccag
      181 ttacggctgg gccgggttcc ccgaggacgg tcgcacagcc aaggaggtca tcgccaaggc
      241 cgacgaagca atgtaccgcg ccaaaagacg gtcgcgtgac agtttcgaga tggcctgaca
      301 tgatcgcccg gtatctcgat acttttcccc gcatcgacgg cagcgcggtc atcttccgca
      361 gcgccaccat catcggcgat gttacgatcg gccctgaggc gaacatctgg ttcggcgcca
      421 tcgcacgagg cgatgtcaac cacatccgta tcggcgcacg caccaatgtg caggacaacg
      481 ccgtcataca tgtcaccgcg gcgaagtatc cgaccgagat cggcgacgag gtgacgatag
      541 ctcaccatac cgtcatccac ggctccatca taggtgaccg gacgctcatc ggtatggggg
      601 cggtgctcct cgacgggtgc cgggtaggct ccgaggtcat ggtggccgcc ggatcgctcc
      661 tgcgcaccgg caccgtcatt cccgacgggg tcctcgtcgc ggggaacccg gcgacggtca
      721 agcggcccct gaccgaagcg gaacgacttt tcctgaaacg gtcggccgac aactatgtaa
      781 aatactctcg caactatttg acttcttcac ccgacacact atattctctt gaagaattga
      841 agaacggggc tcaatggtag gtagttacag tggcataatg aaggagcgaa caatggctag
      901 atcattggtg ttctctgtta tcctttttct caccgtcctc gtttcggcgc aggaaaagga
      961 gaccatgctg accgtgcttt tcatgaatga tatccatgga atggcttggc agtatgacga
     1021 gtccgacaat cccggcatcg gcgggctcgc cgccgtcaag acgctcgtgg accagatccg
     1081 tgccgaggtg cagggcaagg ggggagatgt gctggcgctc atggcgggcg atatcgcgct
     1141 cggcgacccg cgcagcaatg tctgcaagaa ccttccggtc gtgaagggga tgaacctcgt
     1201 cggcttcgat gcgacggtcg tcggtaacca tgacctcttc ttcggcatag acaccttcaa
     1261 cgagatgaag aaagagtcca agttcgactg gatatcggcg aatatctaca aagagggcgg
     1321 caccaagccg ctcgccgacc gcgaatatat cgaaaagacc ttcgacagcg ggctcaaggt
     1381 cgcgatactc ggactgtcga cccgcgagat agaacagata accaccgccg gcctctcagg
     1441 cgacatcgtg gtcaccgacc cgatacagga ggcgaagacg cgggtgcctc ttctcaagaa
     1501 gaacaatcac ctggtgatcg cgcttaccca cctcgggtac ttcgacaccg acaaaagtta
     1561 cgacgggttc gagggcgaca acttcctcgc gcgctcggtg ccgggcatcg accttatcgt
     1621 cggcggccac acccaccgcc aattctacgc gccggtcaag ataggcgact cgctcatcat
     1681 ccagaccgag ggcatgggca aatggctcgg ccgattcgac ctgttcgtaa aaggcggcaa
     1741 gatcaccaag aactacttca agatgtaccc catcaacctc aagaaaaagg tggggagcgg
     1801 caacaacgta tcataccagt tcatggaaaa agagatcaag gagaataagg cgatgctcga
     1861 catgctcgcc ggcttcaagt gcgaattctc cgaaacgctc ctcggcacca ttgagacccc
     1921 gctcgcgggt taccgcgaag aggcgcgatt caaggaaata gagctcggcg acatcatcgc
     1981 cgacatcatg cgtgagcgtg gcaagaccga catcgccttc ttcaacgccg gctcgatacg
     2041 gcagggcctg ccggccggca agataaccga acgggaattg tactcgatgt tccccttcct
     2101 cgacacgctg ttcgtcggca agataaaagg caaggaactg caggatgttc tcgactattt
     2161 cgcacagaaa ggccaagggg ccggcggctt cctgcaagtg tcggggctct cgatgaagat
     2221 atacaagggg tccgcgctcg acatcaagat cggtggtaag ccgctcgata agaacaaggt
     2281 ctataccctc gcgataaaca gtttcatcgc cagcggcggc gacggctaca ccatgctcaa
     2341 ggacctgaag aacaagaagg acacattcat ctccctgcca gccatattgg tcgagtacct
     2401 caaaaagaac aaaaaattcc ccaaaccgga gatggggcgc ctcatcatcg tgcaataatg
     2461 acccgttccc gtatcccgag ctatcccacc gatactaaag ggaattgacc catgacgacg
     2521 gtcgtcggca tcggcaacgc actcgtcgat atcctcgtaa cgcttcccga cgactctctc
     2581 ctgcatcgcc tggaccttcc ccgcgggacc atgaatctcg tcgatcgcga tcggtcccgc
     2641 gcggtactcg acgcgaccgg tcgactctcc cgcacggtca gttcgggcgg ctcggcggca
     2701 aacaccgtac atggcctgac ccgactcggc gtcccctgct cctttgtcgg caaggtgagc
     2761 gacgacg
//
LOCUS       DAOQUE010000392         2761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_43432_length_2761_cov_3.614930, whole genome shotgun sequence.
ACCESSION   DAOQUE010000392 DAOQUE010000000
VERSION     DAOQUE010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43432_length_2761_cov_3.614930"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PKH10_10845"
     CDS             complement(<1..805)
                     /locus_tag="PKH10_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNT28658.1"
                     /translation="MDHIAAFKEYLLYEKRYSSHTAESYGADIASLQKHFSPRKMPLS
                     TLTTDDLKSYLGELFGDLTASSLHRKISAIRHFYSFLHKRGELEENPAAALVSPRKEK
                     YLPTCLTREEMDKILNHPYPHTEKGLRDKAIMELFYSSGLRVSELVSLRIRDVDIAQG
                     VVRVFGKGKKERLTPLTVDAANAIHDYLLFRGGPKEPTARLFLNLRGGERLTARAVEY
                     LMEELVKEAGVFRHVSPHMLRHSVASHLLENGMNIRYLQSMLGHSNLSTT"
     gene            complement(920..2731)
                     /gene="tkt"
                     /locus_tag="PKH10_10850"
     CDS             complement(920..2731)
                     /gene="tkt"
                     /locus_tag="PKH10_10850"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNT28659.1"
                     /translation="MLSNGHASMLIYALLHLSGYDLAMSELRNFRQLHSKTPGHPEVD
                     MTPGVETTTGPLGQGISNAVGMALAEKLLAHEFNRPGHTVVDHRTYVLCGDGDLMEGI
                     SYEAAALAGHLGLGKLIVLYDSNDISLDGPTSLTFTEDVVKRFEACGWQVRTVQNGDT
                     DISAIDRAIAASKMDAGRPSLIVVKTTIGYGAPNKQGKESAHGAPLGEKEAPLARAAL
                     GHATTPPFTVEKDVYHHFSVIAERGKARHAAWKIAAEKWAKAFPELATEWNNGINLDA
                     GTLFEQALPAFKPGDKIPTRKASGMALNAVAKHVPWLVGGDADLSCSTLTAIENGGDF
                     SKDNPAGRNIRFGVREHAMGAIANGIACHGGLRPYTATFFSFVDYMRPAVRLASLCKL
                     PVIFIFTHDSLAVGEDGPTHQPVEQLASIRCIPGLTVIRPSDATEVNAAWELIMRHTG
                     GPVALILSRQALPVLDRTALAPAEELEHGAYLLSDAIDPRATLIATGSEVALALDARR
                     LLAEQGIAVRVVSMPSQDLFDRQDTAYRERILPRHLPTIAIEAGSTSGWHRYVGDTGL
                     AIGVDRFGASGPGDTVMAHFGFTAGAVAERVKDLLSH"
BASE COUNT          483 a          774 c          942 g          562 t
ORIGIN      
        1 cggtggtcga cagattgctg tgaccgagca tgctctgcag ataacggatg ttcatgccgt
       61 tctcgagcag gtgcgaggcg acgctgtggc ggagcatgtg cggggaaacg tgccgaaaga
      121 cccccgcctc tttcaccagt tcttccatga ggtattccac cgcgcgcgcc gtcagccgct
      181 ctccgccgcg caggttgagg aacaggcgcg cggtcggttc tttcggtccg ccgcggaaga
      241 gcaggtaatc gtggatggca ttggccgcat ccacggtgag cggggtgagt cgttcctttt
      301 tccctttgcc gaacacgcgc accactccct gcgcgatatc gacatcgcgg atacgaagcg
      361 ataccagttc gctgacgcgc aggccggaac tgtaaaaaag ttccatgatc gccttgtcgc
      421 gcaggccttt ctcggtatgc ggatacggat ggttgaggat cttgtccatc tcctcgcggg
      481 tcaggcaggt gggaagatat ttctccttgc gaggcgagac gagggcggcg gcgggattct
      541 cctccagttc gcctcgtttg tggagaaagg aataaaagtg gcgtatcgcc gatatcttcc
      601 ggtgaagaga agaggcggtc aggtcaccga acagttcgcc gagataggat ttgaggtcgt
      661 cggtcgttag ggtagagagg ggcatctttc gaggagagaa gtgcttctga agcgacgcga
      721 tgtcggcgcc gtaactctcc gcggtatgag atgaatagcg tttttcgtac aacaggtatt
      781 ctttgaatgc cgcgatatga tccatgactt ctgcctcaaa tatcgacgat tcttatattt
      841 ttttgttacc aaatcaagtt ttcataaaaa atatggtgtg ttatcgcaca gaggtttgat
      901 gggggaatat taaacgatat cagtgggaaa gaaggtcctt gacccgttcg gcgacggcgc
      961 cggcggtgaa tccgaagtgg gccataacgg tgtcgccggg acctgaggcg ccgaagcgat
     1021 cgacgccgat ggcgagcccg gtatcgccga catagcggtg ccacccggag gtgctccccg
     1081 cctcgatggc tatcgtgggc aggtgtcggg gcagtatccg ttcgcggtag gcggtatcct
     1141 gccggtcgaa gaggtcctgc gagggcatac tgacgacgcg gaccgcgatg ccctgttccg
     1201 cgagtagtcg ccgggcatcg agcgcgagcg cgacctcgga accggtcgcg atgagggtgg
     1261 cgcgcgggtc gatggcgtcg gaaaggagat aggcgccgtg ttcgagttcc tcggccggcg
     1321 cgagcgccgt gcggtcgagc accgggagcg cctgccgcga aaggatgagc gccaccgggc
     1381 cgccggtgtg acgcatgatg agttcccacg ccgcgttcac ttcggtcgcg tcggacggcc
     1441 ggatcaccgt gagtcccggg atacagcgga tggaggcgag ttgttcgacc ggctggtggg
     1501 tcgggccgtc ctcgccgacc gcgagactgt cgtgagtgaa gatgaagatg accggaagtt
     1561 tacagagcga cgcgagccgg acagcggggc gcatatagtc gacgaaactg aaaaaggtcg
     1621 cggtgtaggg gcgcaggccg ccgtggcagg cgataccgtt ggctatcgcg cccatcgcat
     1681 gctcccggac gccgaaacgg atgttgcggc cggcggggtt gtccttcgag aagtcgccgc
     1741 cgttctctat cgcggtgagc gtagagcaac tgaggtcggc gtcgccgccg acgagccaag
     1801 ggacatgctt ggcgaccgca ttgagggcca tgccgctggc cttgcgcgtc ggtatcttgt
     1861 cgcccggttt gaatgcggga agcgcctgct cgaaaagggt gccggcgtcg agattgatgc
     1921 cgttgttcca ttctgtagcc aattccggga aggccttcgc ccatttctcg gcggcgatct
     1981 tccacgcggc gtgcctggct tttccccgct cggcgataac ggaaaaatgg tggtagacat
     2041 ccttctcgac ggtgaagggc ggggtggtcg cgtgaccgag cgcggcgcgg gcaagcggtg
     2101 cctccttttc gcccagcggc gcgccgtggg ccgattcttt gccttgcttg ttcggggcgc
     2161 cgtagccgat ggttgtcttc acgacgatca gcgaggggcg gcccgcgtcc attttcgagg
     2221 cggcgatggc gcggtctatc gccgatatgt ctgtgtcgcc gttctggacg gtgcgcacct
     2281 gccagccgca ggcctcgaac cgtttgacga catcttcggt gaaggtgagc gaggtcggac
     2341 cgtcgagcga tatgtcgttc gagtcgtaga gcacgatcag ttttcccaga ccgagatgcc
     2401 ccgcgagcgc cgccgcttcg taacttatcc cctccatcag atcgccgtcg ccgcacagga
     2461 cataggtgcg gtggtcgacc acggtgtggc cggggcggtt gaattcgtgc gccagcagct
     2521 tctcggccag cgccatgccc acggcattgc tgatgccctg gcccagcggg ccggtggtgg
     2581 tctcgacgcc cggcgtcatg tcgacctcgg ggtggcccgg cgtcttgctg tgcagctggc
     2641 ggaagttgcg cagctcgctc atcgccaggt cgtagccgct caggtgcagc agtgcataga
     2701 tcagcatcga cgcgtgcccg ttgctcagca cgaagcggtc gatgctgatc tatgcactgc
     2761 t
//
LOCUS       DAOQUE010000393         2756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_43563_length_2756_cov_3.344317, whole genome shotgun sequence.
ACCESSION   DAOQUE010000393 DAOQUE010000000
VERSION     DAOQUE010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43563_length_2756_cov_3.344317"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2405)
                     /locus_tag="PKH10_10855"
     CDS             complement(<1..2405)
                     /locus_tag="PKH10_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28660.1"
                     /translation="MSRAQEKFCENGPVRYIRDGPWHTEVDLRSLFTALSLLCAFSLL
                     SAADTPRYLVHRTGDLVLYFSSDIDPRLIPYFIEKTEMAEQFLFSLYGWKADRRIPIV
                     FDRETDDANGWSSVYLKDAIRLLIYPPEEFSVLANHKDYVLNLAVHELTHSLQIGLVN
                     GVPKWVNYVFGNLFFPSQLMPGWMLEGVTVYSESAIDGTGRGHSPVWRAWFDSFFRKG
                     NELTLGELSGSNDHWMGGHIPYLYGSFFYDFVTRRHGLERMALFFDEMSDNAIPFLAE
                     YEAKKVFGESFWHMYQEFLQKQRERLFDRDATAQAPFEIGERYHDIMVDLSHPDRYVY
                     YGVTNGRRAVYEFDGTAATRLMTLPTASTFAAGDDQFLATVPVSVDNDRTRSDLFLFD
                     MKRQAITRITRGESARYPIFLHDGRIGCFTVTDGLHRFRIYDRKGALLRTYDLLPFDA
                     VYTPSFDPTGERVLFTGNLRGADKDIFLLSLDDGAVTRLHLPGDQYGAGFASAHQLLF
                     STEDDGRIVPVLLDLRTDSLSRLYEPVSIAIYPKILADRLFFIGFDNDGYFPAWVPLR
                     PTAAVPITVERTTVAASATGTDSVAPAIEETHGWEGAWPTALIPDYNGGLSLQKIGVT
                     LFAESDTGRRGYEISFGKAFGDTDTYEARLTYRDDDIMPSFYWQAYYGWYRATFGDPE
                     HITYRGAVHDLRTHLSTSSRFNTLWFLTPSYAYRATHSVSASIGFRFRQETVENDRDD
                     PTILPYDPRKSYSVQSGASYNFHFNFSPGSYLLFSPMDQSILRLPATISRDLWTGENL
                     LTLT"
     gene            2479..>2756
                     /locus_tag="PKH10_10860"
     CDS             2479..>2756
                     /locus_tag="PKH10_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28661.1"
                     /translation="MIIYVAFDDTDTLDCGRGTGKLARWYGGELPDGCRLHALVRQQL
                     LVAADIPYTSHNSSLVCMIEAPGREVIPELIRRGAAHIKKHFVEGSDPG"
BASE COUNT          554 a          741 c          954 g          507 t
ORIGIN      
        1 gtgagggtca ggaggttctc gccggtccag agatcgcgcg atatcgtcgc ggggaggcgg
       61 aggatactct ggtccatcgg cgagaagagc aggtaacttc ccggcgagaa attgaaatgg
      121 aaattgtagg aggcgccgct ctgcacgctg tagctcttgc gcgggtcgta gggaagtatc
      181 gtcgggtcgt cgcggtcgtt ctcgacggtc tcttgacgga accggaaccc gatgctcgcc
      241 gagaccgaat gggtcgcccg gtaggcgtag gatggcgtca ggaaccagag cgtattgaac
      301 cgggacgagg tcgaaaggtg ggtgcggaga tcgtggacgg cgccgcggta ggttatatgt
      361 tccgggtctc cgaaggtggc gcggtaccag ccgtagtagg cctgccagta gaaggacggc
      421 atgatgtcgt catcgcgata ggtcagccgt gcctcgtagg tgtcggtgtc gccgaaggcc
      481 ttgccgaatg atatctcgta gccgcgacgg ccggtgtccg actcggcgaa cagcgtgacg
      541 cctatcttct ggagggacag gccgccgttg tagtcgggga tgagcgccgt cggccacgcc
      601 ccttcccacc catgggtctc ttcgattgcc ggggcgacgc tgtccgttcc ggtggctgaa
      661 gcggcgacgg tggtccgctc cacggtgatc ggcacggcgg cggtgggacg cagggggacc
      721 cacgcgggaa aatagccgtc gttgtcgaac ccgataaaga acaggcggtc ggcgagtatc
      781 ttggggtaga tcgcgatcga taccggttcg tacagccgtg aaagggaatc ggtacgcagg
      841 tcgagaagga ccggcacgat gcgaccgtcg tcctcggtac tgaagagaag ttggtgggcg
      901 gaggcaaacc cggcgccata ctggtcgccc ggcagatgga ggcgggtgac cgcgccgtcg
      961 tcgagcgaaa ggaggaaaat gtccttgtcg gcgccgcgca gattgccggt gaacaacacc
     1021 cgttcgccgg tcgggtcgaa cgagggggta tagacggcgt cgaacggcag gaggtcatag
     1081 gtgcggagca gagcaccttt ccggtcgtag atgcggaagc ggtgcaggcc gtcggtgacg
     1141 gtgaaacagc cgatcctccc gtcgtgaagg aatatcgggt agcgtgccga ctcgccgcgg
     1201 gtgatccgcg tgatggcctg tcgtttcatg tcgaacagga acaggtcgct ccgtgtccgg
     1261 tcgttgtcga cggatacggg gaccgttgcg aggaactggt cgtcaccggc ggcgaaggtg
     1321 gatgccgtgg ggagcgtcat gaggcgggtc gcggcggtgc catcgaactc atagacggcg
     1381 cgccgtccat ttgtcacgcc gtagtacaca tagcggtcgg gatgcgagag atccaccatg
     1441 atgtcgtgat atcgttcgcc gatctcgaag ggggcctgcg ccgtcgcgtc gcggtcgaaa
     1501 agccgttcgc gctgtttctg gaggaactcc tgatacatgt gccagaagct ctccccgaac
     1561 acctttttcg cctcgtattc ggcgaggaac ggtatcgcgt tatccgacat ctcgtcgaaa
     1621 aagagcgcca tccgttccag cccgtgacgc cgggtgacga aatcgtagaa gaaggagccg
     1681 tacaggtaag ggatatggcc gcccatccag tggtcgttcg accccgaaag ttcgccgagc
     1741 gtcagttcgt tgcctttgcg gaaaaaagag tcgaaccacg cccgccagac cggggagtgc
     1801 ccccgtccgg taccgtcgat ggccgattcg gaatagacag tgaccccctc gagcatccag
     1861 ccgggcatga gttgcgaggg gaagaacaga ttgccgaaca catagttgac ccatttcgga
     1921 acgccgttca ccagaccgat ctggagcgag tgggtgagtt cgtggacggc gaggttgagt
     1981 acataatcct tgtggttcgc gaggaccgaa aattcctcgg gagggtatat gaggagccgg
     2041 atggcgtcct tgaggtagac cgaggaccag ccattcgcgt cgtcggtctc gcggtcgaac
     2101 acgatcggga tgcgtcggtc cgccttccag ccgtagagcg agaagaggaa ctgttcggcc
     2161 atctcggtct tttctatgaa gtaggggatg aggcgggggt cgatgtcact cgagaaatag
     2221 aggacaaggt caccggtgcg gtggacgaga tagcgaggtg tatcggcggc ggagagcaga
     2281 gagaaggcgc aaagaagcga gagtgcggta aaaagtgacc tcaggtcgac ctccgtgtgc
     2341 catgggccgt cgcgaatata ccgtacgggt ccgttttcgc aaaatttttc ttgtgcgcgg
     2401 ctcatccggg gccggcaaaa taattgacaa gaaagccccg aaaagtaccg tgaagcggta
     2461 tgtttcctag gggttccgat gatcatctat gtcgccttcg acgacaccga cacgctcgac
     2521 tgcgggcgcg gcaccggcaa actggcccgc tggtacggcg gtgaacttcc cgacgggtgt
     2581 cgcctgcacg ccctcgttcg tcagcagcta ctcgtcgccg ccgatatccc gtacacctcg
     2641 cacaacagtt cgctcgtctg catgatcgag gcgccgggac gcgaggtgat accggaactc
     2701 atccggcgtg gcgcggccca catcaaaaaa catttcgtcg aagggagcga cccggg
//
LOCUS       DAOQUE010000394         2732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_44155_length_2732_cov_3.465820, whole genome shotgun sequence.
ACCESSION   DAOQUE010000394 DAOQUE010000000
VERSION     DAOQUE010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44155_length_2732_cov_3.465820"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PKH10_10865"
     CDS             <1..573
                     /locus_tag="PKH10_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28662.1"
                     /translation="TMNDTDAAVETDTVADNDTIVDADTLPAESDTVTDTDVDLTDDT
                     GDEDADVAFDDAPPAVDDSLLTDADTDTVTDTAPDTDTSCTGCACEDCSGHGTCTTAN
                     GYVECICDTGYGGGYCQQCDIEWQDHDGDGICEADDCAHATDPIDGWLDCGDHGTCDD
                     WGFEADCECDMYWTDMSGECDYCFGDPEDC"
     gene            608..1144
                     /locus_tag="PKH10_10870"
     CDS             608..1144
                     /locus_tag="PKH10_10870"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT28663.1"
                     /translation="MKRYQLLFILVPTVFLLCSGCRFTTADPVKGETVAIDKKSGLMW
                     HKSANLNRYPWSAAKKYCSDLSYAGYDDWELPSREEIATLWDPETKGPNSMFPFKLEP
                     ANYWTRDEEVVTDPAERAKLLNNRPMIGPTEEYMRKNPDSSFEDELFRAYAIRFTDGS
                     MDAYRKLRQSYLLCVRKE"
     gene            complement(1314..1484)
                     /locus_tag="PKH10_10875"
     CDS             complement(1314..1484)
                     /locus_tag="PKH10_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28664.1"
                     /translation="MSQQREFLSRLIERFDALGIVQMQGDTLDRDYLRRWATEIGVGD
                     LLGKLLAIGADI"
     gene            complement(1481..1720)
                     /locus_tag="PKH10_10880"
     CDS             complement(1481..1720)
                     /locus_tag="PKH10_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28665.1"
                     /translation="MRGVALRSDTTPEAERVQIDILRRLSPGARALLGAELSEMAYDA
                     CLSGLTARHKGEDRAQIIHRYIRLVHGIMLPPEIA"
     gene            complement(1751..1826)
                     /locus_tag="PKH10_10885"
     tRNA            complement(1751..1826)
                     /locus_tag="PKH10_10885"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1791..1793),aa:Met,seq:cat)
     gene            complement(1921..>2732)
                     /locus_tag="PKH10_10890"
     CDS             complement(1921..>2732)
                     /locus_tag="PKH10_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28666.1"
                     /translation="SPADSAAYYDATPDINTCGYKCATGYSWNGSACVAAYCGDGISG
                     NSTIPTFTEGFEGSFPPAYAHQASQFTSDASGWERSSTRYHGASYSMKNVAIDDDGFS
                     AIFLQKYTTIVTDVSLCFYYTGQSESGYDFFFVFKSVQDFLDAATTAYNDAPSAVSNY
                     LFRATGDKSSQWYKQCVTVPAGYPTVVFAYMKDYADATGWDAFYVDDVTFHPAVTEQC
                     DGGTTNCTNLGYSVAGTVNCGSDCTWASGESGEKGCNNGEEPPSCSLVLVF"
BASE COUNT          597 a          750 c          835 g          550 t
ORIGIN      
        1 acgatgaacg acaccgacgc cgctgtcgag accgacacgg tcgccgacaa cgatacgatc
       61 gttgatgccg atactctccc ggccgaaagc gacacggtga ccgatacgga tgtcgatctg
      121 accgatgaca ccggcgatga agatgccgat gtcgcgttcg acgatgcgcc gcctgcggtg
      181 gatgactcac tcctcaccga cgcggatacc gacacggtca ccgacaccgc acccgacacc
      241 gacacctcat gcaccggctg cgcctgcgag gattgttcgg gacacgggac ctgcacgacg
      301 gcgaacggct atgtcgagtg catctgtgac accggctacg gcggaggtta ttgccagcag
      361 tgcgacatcg agtggcagga ccacgacggc gacggcatct gcgaggccga cgactgcgcc
      421 catgcgaccg atccgataga cggttggctt gattgcggcg accacgggac ctgcgacgac
      481 tggggcttcg aggccgattg cgagtgcgat atgtattgga ccgatatgag cggcgagtgc
      541 gactactgct tcggcgatcc ggaagactgc tgaacggtta aaacaagtcg atcttgagga
      601 ggctgttttg aaacgctatc agttgctctt tattctggtg ccgacagtgt ttttactttg
      661 ttccggttgc cgcttcacga cggcggaccc ggtgaaggga gaaacggtgg ccatcgacaa
      721 gaaaagcggt ctcatgtggc acaagtccgc gaacctcaac aggtatccgt ggtcggccgc
      781 gaagaaatat tgctccgatc tctcgtacgc cggttatgac gattgggaac tgccttcgcg
      841 cgaagagatc gcgacgctct gggaccccga gacgaagggg ccgaactcga tgttcccttt
      901 caaattggag ccggcgaact attggacaag ggacgaagag gtagtaaccg acccggccga
      961 aagggccaag ttactcaaca acaggccgat gatcgggccg accgaagaat atatgcggaa
     1021 gaaccccgac tcttcttttg aggacgaact attccgtgca tacgccatcc ggttcaccga
     1081 cggttcgatg gatgcttacc ggaaacttcg ccaatcctat ctgctgtgcg tgcggaaaga
     1141 atgatcccgg cgcgtaacga actgctctga tcccttcgct ttcaatttag tcaggcgggt
     1201 gttgtagggc tcacgggcga tggcgccctc taggaaggcg tcagtaagcc gcgcgatgga
     1261 ggcgcgaagg gtggagaaga gaaaatgtgt acggtttctt tgggaaggat caatcagata
     1321 tctgcgccga tagctagcaa ttttcccaga agatcgccga cacctatctc ggtggcccac
     1381 cggcgcaagt aatcgcggtc gagggtatcc ccctgcatct gtactatgcc gagcgcgtcg
     1441 aatcgttcaa tgagtcggga aagaaattcg cgttgctggc tcatgcgatc tcgggcggca
     1501 gcatgatgcc gtgaacgagt cggatatagc ggtggatgat ttgagcccga tcctcgcctt
     1561 tatgtcgcgc cgtaagacct gaaagacagg catcataagc catttcagaa agttcggctc
     1621 cgagaagggc gcgcgcaccc ggagaaaggc gacgaaggat gtcgatctgt acgcgttcag
     1681 cttcgggtgt cgtgtccgac cgcaaggcga cacctctcat gccggtcagg gcgaccgttg
     1741 ataaaaaaaa tggtcgcggg gcttggactt gaaccaagga ccttcgggtt atgagcccga
     1801 cgagctacca tctgctctac cccgcaacct tcggaagcag gggcattata tgaggcgagt
     1861 cccgtttgtc aatagctatg aggaaaaaag cgggggaatg ccgttcgggg gagaatttct
     1921 ttagaatacc aagaccagcg agcaactggg cggctcttct ccgttgttgc aacccttttc
     1981 tcccgattct cccgatgccc atgtgcaatc ggagccgcag ttcaccgtcc cggcaacgct
     2041 gtagccgagg ttggtgcagt tggtcgtacc gccgtcacac tgctcggtca ctgccgggtg
     2101 gaaggtgaca tcgtccacat agaaggcgtc ccacccggta gcgtcagcat aatccttcat
     2161 gtaggcgaac acgaccgtcg ggtacccggc gggcacggtg acgcattgtt tgtaccactg
     2221 gctactcttg tcgccggtcg ctcggaatag gtagttcgat accgccgacg gggcgtcgtt
     2281 ataagcggtg gtcgcggcat cgaggaaatc ctgaacgctt ttgaatacga agaagaagtc
     2341 gtaccctgat tccgactgtc cggtgtagta gaagcagaga gaaacatcgg tgacgatagt
     2401 agtgtacttc tgaaggaata tcgcggagaa cccatcgtcg tcgatagcca cattcttcat
     2461 ggagtaggag gcgccgtggt accgagtgct gctccgttcc catccggaag catcgctagt
     2521 gaactggctc gcctgatggg catacgccgg cgggaaggaa ccctcgaacc cctcggtgaa
     2581 ggtcgggatc gtgctgttgc cggatatgcc gtcgccgcag tacgcggcca cgcacgcgga
     2641 gccgttccac gagtagcccg tcgcgcactt gtatccgcag gtgttgatat cgggtgtcgc
     2701 atcgtagtag gccgcgctgt cggccgggct cc
//
LOCUS       DAOQUE010000395         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_44309_length_2726_cov_4.213778, whole genome shotgun sequence.
ACCESSION   DAOQUE010000395 DAOQUE010000000
VERSION     DAOQUE010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44309_length_2726_cov_4.213778"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKH10_10895"
     CDS             complement(<1..405)
                     /locus_tag="PKH10_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28667.1"
                     /translation="MCRHLVGISVTRTLARMFLVVVLCAIPLRSVSSGEVRTFVLNDA
                     VRFYAAGEYPQALAEIEKVKKLLEGTSMADEAVLLRAQIVAFSKTLEKVHEAVRAGEG
                     ECLLRTLANADTLDVIIAPRGSVWRSQSKTIRA"
     gene            complement(654..1121)
                     /locus_tag="PKH10_10900"
     CDS             complement(654..1121)
                     /locus_tag="PKH10_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28668.1"
                     /translation="MEVKRAPIDLKKFVVPSVRLETIPVPPDRKEKKLPPLPLDLDFN
                     VFAVPNEPQLLRIVVRILANGQGKLPGYRFEIVAEGLFEFATGPSATGGEKREETVFF
                     ALPLLIHSTRAYLLNLTAAGPYGPFALPMPDVKDLLEKKRAAEAATKAKDSSA"
     gene            complement(1135..>2726)
                     /locus_tag="PKH10_10905"
     CDS             complement(1135..>2726)
                     /locus_tag="PKH10_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNT28669.1"
                     /translation="LDTCLGWDTVTCEEYEYDCGDGDVDFPEVCEIGELTDCVDIDPM
                     KYKAGKAYCLDTCLGYDTATCEEIEEDDDTTYPDTADVQPDPDVVDDWIPDTTDVQPD
                     PDTVDIDNAEADILPDVDTADADYFEDDVVPDNDSAGPCGSIRLDGSTGYVEVAHNAL
                     LDLAGNWTIEAWVNQDSDTDQAAIIRKGGTTSYPAYYLYGANYGVPYGGYYYSAGSFY
                     SASGVTEPANGDWYHVALVKNGTALTIFVDGEPSAATTAASASYANSENLFFGSRRSS
                     TPSYFDGLIDEIRISNVARYNSSFTPQQRFASDGNTIGLWHFEEGANNIAYNSAGDTL
                     DGLLVGGVEWENACAAAGPIIQPDDDVVPDEDTAGAVIDTVGSTEPMTGGTSRMRGNF
                     YSCDTDRTLTEIEVYLTYTGTPTIYFEVYESTTLDGTYYPLESVTATPSMSDGAFYSS
                     GDLSVPLTAGNYYYVGVRWGSSYSVGYAADTAPAVGDYPTAFGTWEDGMLDDGVTSAP
                     ASFLYDDVSYGYAYYQRLTTQ"
BASE COUNT          560 a          816 c          799 g          551 t
ORIGIN      
        1 cgcacggatg gtcttgcttt ggctccgcca gacgcttcct cgcggcgcga taatgacgtc
       61 gagcgtatcg gcgttcgcga gagtccgcaa caggcactcg ccctctccgg cgcgcactgc
      121 ctcatggacc ttttcaagcg tcttgctgaa ggcgacgatc tgcgcgcgta gcaacaccgc
      181 ctcgtcggcc atcgaggtgc cttccagcag ttttttgacc ttctcgatct cggcgagcgc
      241 ctgcggatac tcgccggccg cgtagaaccg gaccgcatcg ttcaacacga aggtcctcac
      301 ctcgcccgac gacactgatc ggagaggaat tgcacacagg acaacgacga ggaacatcct
      361 cgccagtgtc cgtgtcacgc tgatcccaac aagatgacgg cacatgacct cctccttctc
      421 accagtgagt aaagaccgcg cttttctcga ggagtcggag tgtcggaaca ggagcagatt
      481 cttaccgtgg agtagccatc caaacgctga gaaggaacca ccgttggtcc tgacggacga
      541 gggcggcgag cgcggagatc cataccatcg agtggcaaag agcggagggg tcctcaatcg
      601 aatccgattt cagtataaca ctttcgtgcg agaatgcaag cggtatctcg aggtcaggcc
      661 gaagaatcct tcgccttggt ggcggcttcg gccgcccgtt tcttctcgag aagatccttc
      721 acatcgggca tcggaagcgc gaagggacca taggggcctg ccgccgtgag attcagcagg
      781 tatgcccgcg tcgagtggat gaggagcggg agcgcgaaaa aaacggtctc ttcgcgtttc
      841 tctccaccag ttgccgatgg cccggtcgcg aactcgaaca gtccctcggc gacgatctcg
      901 aaccggtagc cgggcagttt cccctgaccg ttggcgagta tccgcaccac tattcgcaac
      961 agttgcggct cgttaggcac cgcaaagaca ttgaagtcaa ggtcgagcgg caacgggggc
     1021 agtttcttct ccttgcggtc gggtggcacc gggatggtct cgagacgcac cgacggcacg
     1081 acgaatttct tgaggtcgat gggggcgcgt ttgacttcca tgatccgctc cgtcttactg
     1141 cgtcgtcagc cgctgatagt aagcatagcc ataggaaaca tcatcataga gaaaactggc
     1201 gggggcgctg gtaacaccgt catcaagcat cccgtcctcc catgtaccga aggcagtagg
     1261 ataatcgcca acggcggggg cggtatcggc cgcataaccg acgctgtagg atgagcccca
     1321 gcgcacgccg acatagtaat aattcccggc ggtcaagggg accgaaaggt cgcctgatga
     1381 gtagaaagca ccatcggaca tgctaggggt cgcagtcacg ctttcgagtg ggtagtaggt
     1441 gccgtcaagc gtggtcgatt cgtagacctc gaaatagatc gtcggtgtac cggtgtaggt
     1501 gagatacacc tctatctcgg tcagcgtccg gtcggtgtcg caagaataga aattgccacg
     1561 catgcgactc gtgccgccgg tcatgggttc ggtactgccg accgtgtcga tgaccgcccc
     1621 tgcggtgtcc tcatcaggca ccacatcgtc gtcgggttgg atgatcggtc ccgccgccgc
     1681 gcaggcattt tcccattcta ccccgccgac aagcagaccg tcgagcgtat cgcccgccga
     1741 attataggcg atgttgttcg cgccctcttc gaaatgccac agaccgatgg tgttgccatc
     1801 gctcgcgaat cgttgctgcg gcgtgaagga ggaattgtac cgcgccacat tcgatatccg
     1861 tatctcgtcg ataaggccgt caaaataact cggcgtactc gaccgacgcg aaccgaagaa
     1921 aaggttctcg ctattcgcgt acgaagcgct ggcggcggtc gtcgcggcac tcggctcccc
     1981 gtcgacgaag atagtgagcg cggtcccatt cttgacgagc gcgacatggt accaatctcc
     2041 gttcgccggt tccgttacgc cggaggcaga ataaaaagaa ccggcgctgt agtagtatcc
     2101 gccgtaggga acaccgtaat tggcaccgta cagataatag gcggggtaac tcgtcgtgcc
     2161 ccccttgcgg atgatcgccg cttgatcggt gtcgctgtcc tgattcaccc acgcttcgat
     2221 ggtccagttg ccggccaggt cgagcagggc gttgtgcgcc acttcgacat agccggtcga
     2281 gccgtccaga cggatcgatc cacacggacc ggcgctgtca ttatcaggga ccacatcgtc
     2341 ctcgaaataa tccgcatcag cggtatcgac atcgggaagg atatcggcct cggcgttgtc
     2401 gatatcaacc gtatcggggt caggctgcac atcggtcgtg tcgggtatcc agtcatcgac
     2461 cacatccggg tcgggctgca catccgccgt gtcgggatag gtcgtgtcgt catcctcctc
     2521 gatctcctcg caggtcgcgg tgtcatagcc gagacaggtg tccagacagt aggccttacc
     2581 cgccttgtac ttcatcgggt ctatgtccac gcaatcggtg agttccccga tttcacacac
     2641 ctcgggaaag tcgacatcgc cgtcaccgca atcgtattcg tactcctcgc aggtcaccgt
     2701 gtcccagcca agacaggtgt ccagac
//
LOCUS       DAOQUE010000396         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_44570_length_2715_cov_4.542481, whole genome shotgun sequence.
ACCESSION   DAOQUE010000396 DAOQUE010000000
VERSION     DAOQUE010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44570_length_2715_cov_4.542481"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /gene="eno"
                     /locus_tag="PKH10_10910"
     CDS             complement(<1..422)
                     /gene="eno"
                     /locus_tag="PKH10_10910"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150542.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNT28670.1"
                     /translation="MHEIIEVRSREILDSRGNPTVEVEVELDSGDIGVAMVPSGASTG
                     VHEALELRDGDKARYLGKGVLKAVENVQRAAQEVVGISALEQRLIDMTLIELDGTDNK
                     SDLGANAMLGISMAAARAAARALRLPLFRYLGGVTSDTL"
     gene            complement(436..849)
                     /gene="cdd"
                     /locus_tag="PKH10_10915"
     CDS             complement(436..849)
                     /gene="cdd"
                     /locus_tag="PKH10_10915"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNT28671.1"
                     /translation="MRKELVKKAVAAALKVRRHARAPYSGFMVGAAVITRSGRIFTGV
                     NVENSSFGATVCAERVALFSAITAGRGDIVAVVVAATLDGHAVSPCGICRQTLADIDP
                     EMRVILVNAQTGEIEKETTVAALLPDRFSFDIPNP"
     gene            complement(842..1684)
                     /locus_tag="PKH10_10920"
     CDS             complement(842..1684)
                     /locus_tag="PKH10_10920"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015734483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNT28672.1"
                     /translation="MASIREAAQFIGEKLADAGLESGRLAMVLGSGLGNFADSIEVRA
                     AIPYRDIPNFPVSTVTGHAGKLLLAAEPQSGRPFWVMQGRVHCYEGYTPQQAVFPIRV
                     LRLLGVETVFLTNAAGGIRDDLLPGSFMLVADHLNLTGMNPLTGPNLDGMGTRFPDMT
                     EAYDPSLRRMMRTVAAEQGVVMAEGVYAGLAGPCFETPAEIRMLEKLGADAVGMSTVP
                     ETIAAHHAGMRVAAVSFIANRAAGKSEGRLSHEEVFGNAKAAEGRFGALMLAAVGRTL
                     GDDA"
     gene            complement(1701..>2715)
                     /locus_tag="PKH10_10925"
     CDS             complement(1701..>2715)
                     /locus_tag="PKH10_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNT28673.1"
                     /translation="HYYGGIKKYQWVTRPLAMHGVVVLENGKKIEVVIGEEEGDPVFT
                     INDLLPHLAQEQMERNARKVIEGENLNILVGSIPYNFKPEASAIKVNILKMLHDRYGF
                     AEQDLVSAELQLVPAGNARDVGLDRSFVGSHGQDDRICAFAGMRALLDLPKSDRNIVA
                     VFFDKEEIGSKGNAGANSNLLELAVAEILGHYGVTDYATVLKALRNSRFLSADVNAGI
                     DPEWAGVVEKMNAAKIGFGVCLTKFTGSGGKSNSSEAHAEFVAQVRKAFNKDGALWQT
                     AELGKVDLGGGGTIAMYIAEYGMDVLDVGTALLSMHSPFEVASKADLYHTYKAYKAFY
                     KYC"
BASE COUNT          482 a          871 c          835 g          527 t
ORIGIN      
        1 agggtgtcgc tcgtcacgcc gccaagatac cggaagagcg gcaggcgcag agcgcgggcc
       61 gcggcgcgtg cggcggccat cgagatgccg agcatcgcgt tcgcgccgag atccgatttg
      121 ttgtcggtgc catcgagttc tatgagtgtc atgtcgatga gccgctgttc gagcgccgag
      181 atgccgacca cctcctgcgc cgcgcgctgg acattctcga ccgccttgag gacccccttg
      241 ccgaggtaac gcgccttgtc gccatcgcgc agttcgagcg cctcgtggac gccggtcgag
      301 gcccccgacg gcaccatcgc gacgccgatg tcacccgagt cgagttcgac ctcgacctca
      361 acggtgggat tgccgcgtga atcaaggatc tcgcgtgatc ttacctcaat gatctcgtgc
      421 atggtgatgc ctccgttatg ggttgggtat atcgaacgaa aaacggtcgg gcagaagcgc
      481 cgcgacggtc gtttccttct ctatctcgcc ggtctgggcg ttgacgagta tgacccgcat
      541 ctcgggatcg atatcggcga gcgtctgccg gcagatgccg cagggactca ccgcgtggcc
      601 atcaagcgtc gcggcgacca ccaccgcgac gatatccccg cgccccgccg ttatcgccga
      661 gaaaagcgcc acccgctcgg cgcagacggt cgcgccgaac gaggaattct cgacattcac
      721 gccggtgaag attcgaccgc ttctggttat caccgccgca ccgaccataa agccggaata
      781 gggcgcgcgg gcatgacggc ggaccttgag cgctgcggcg accgccttct tgacgagttc
      841 cttacgcatc gtcccccagc gtccgaccga cggcggcgag catgagggcg ccgaaccgcc
      901 cctcggccgc cttcgcgttc ccgaacacct cttcgtggga gagccttcct tcgcttttcc
      961 cggcggcgcg gttggcgatg aatgacaccg ccgcgacccg catcccggcg tgatgcgccg
     1021 ctatcgtctc ggggacggtc gacatcccga ccgcatcggc accgagtttt tccagcatcc
     1081 gtatctcggc gggcgtctcg aaacagggac ccgcgagtcc cgcgtagacc ccttcggcca
     1141 tcacgacgcc ctgttcggcc gcaacggtcc gcatcatccg acgcaacgaa ggatcgtagg
     1201 cctcggtcat gtctgggaac cgtgtcccca tcccgtcgag gttaggaccc gtgagcgggt
     1261 tcatcccggt caggttcaga tggtcggcga ccagcatgaa tgagcccgga agcagatcgt
     1321 cgcgaatacc tcccgccgcg ttggtgagaa acaccgtctc gacgccgagc aggcggagga
     1381 cccggatcgg gaatacggcc tgctgggggg tatagccctc atagcagtgg acccgtccct
     1441 gcatgaccca gaagggacga ccgctctgcg gctcggcggc aagcaggagt ttccccgcat
     1501 gacccgtgac ggtcgagacg gggaagttcg gtatatcgcg gtagggtatt gcggcgcgca
     1561 cctcgatcga atcggcgaag ttacccagtc cagaaccgag gaccatcgcg agtcgaccgc
     1621 tctcgagtcc ggcgtcggcg agtttttcgc cgatgaactg cgccgcctcg cggattgatg
     1681 ccatcgccgc gatcgtcatg tcagcagtac ttgtagaagg ccttatacgc cttgtaggtg
     1741 tgataaagat cggccttcga cgccacttcg aacggcgagt gcatcgacag gagcgcggtg
     1801 ccgacatcaa ggacatccat cccgtattca gcgatataca tcgcgatggt gccgccgccc
     1861 cccagatcga ccttgcccag ttcggcggtc tgccagagcg caccgtcctt gttgaaggcc
     1921 ttgcggacct gcgcgacgaa ctcggcatgc gcttcggagg agttcgactt gccgcccgag
     1981 ccggtgaact tggtcaggca gacgccgaat cctattttgg cggcgttcat cttctcgacg
     2041 acgcccgccc attcgggatc gatgccggcg ttgacatcgg ccgaaaggaa cctggaatta
     2101 cggagcgcct tgagaactgt cgcgtagtcg gtcacgccgt agtggcccaa tatctcggca
     2161 acggcgagtt caagcaggtt ggagttcgca ccggcgttac ccttcgatcc gatctcctcc
     2221 ttgtcgaaaa agacggcgac gatgttcctg tcgcttttgg gcaggtcgag cagtgctcgc
     2281 ataccggcga acgcgcagat gcggtcgtcc tgaccatgcg agccgacgaa cgagcggtcg
     2341 agtccgacat cacgcgcatt gcccgccggg accagttgca gttcagcgct gacgaggtcc
     2401 tgttccgcga atccgtaccg gtcgtggagc atcttgagga tgttcacttt tatcgcgctt
     2461 gcttcgggtt tgaagttata ggggatcgaa ccgacaagga tattgaggtt ctctccttcg
     2521 atcaccttgc gggcgttcct ctccatctgt tcctgagcga ggtgcggcag gagatcgttg
     2581 atcgtgaaga ccgggtcgcc ttcctcctct ccgatgacca cctctatctt tttcccgttc
     2641 tcgaggacca ccacaccgtg catcgcgagc ggccgggtga cccactggta cttcttgatg
     2701 ccgccgtagt agtgg
//
LOCUS       DAOQUE010000397         2703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_44869_length_2703_cov_3.766239, whole genome shotgun sequence.
ACCESSION   DAOQUE010000397 DAOQUE010000000
VERSION     DAOQUE010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44869_length_2703_cov_3.766239"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PKH10_10930"
     CDS             complement(<1..969)
                     /locus_tag="PKH10_10930"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28674.1"
                     /translation="MRILDQQEEVRNIARKSRVMLGVIFISLLLLSVRIGYLQLYRGG
                     EHLQKSKNNYLLREKIFPARGDILTADGRTLATTSAQFRIQILPAFFTGDEVEESSMR
                     LASILGLSDRQVGELVEFLSTCRGRCRYTPVTVRDELPKSEMLRLSSYLTDVPGVAVS
                     SSYRRVYPLGITAAHLTGYVGRISRGDLSAYPGYDPESFVGKTGIERSWERELHGVYG
                     ELWQVIDHVGRRIEKQDRSSGEVPPTVPARKGRSVQTAVLSYLQEEAEAALGERAGAV
                     VMMDIHTGDILALYSNPSYDPTLLSGRRISPSVWLEYSESVLRPLTN"
     gene            complement(966..1460)
                     /locus_tag="PKH10_10935"
     CDS             complement(966..1460)
                     /locus_tag="PKH10_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28675.1"
                     /translation="MKRFSLEFHAGLAALFALFSFFLYLFDLPFHYFPHLPFLFTFLC
                     YEKKERMRMVSFLLNGVLFVLLAHDLPLFFIAFLFGHLYVFTRLTLEVTTFQFSLVLF
                     LTTFLSSLLVIADVMIYRFLFTGLAGLADGLAAATVNLAVLLFLFLFRRRPIVSLYTR
                     DAWL"
     gene            complement(1441..2268)
                     /gene="mreC"
                     /locus_tag="PKH10_10940"
     CDS             complement(1441..2268)
                     /gene="mreC"
                     /locus_tag="PKH10_10940"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNT28676.1"
                     /translation="MRKRYLLIALFAVFVFALALLPRLLTEWSWRAPIVRDARIAVSE
                     RVSRLFTSVAILRDLHTVGERNIALEEENARLRLQIMMSEGIKRENEELAKLLKIKDR
                     YSGYTIIPARLLSQSSVNPTRITIHYDGEFAKHLALNAPVVSSMGLVGLVRSFGANSA
                     EVDLITSRHFSLPAILESREECTAILRGNGQTLSIQFFEKVCNEPSAVGRKMLSANLS
                     ENYSLPYIPIGVIGSLIEDENNILFYRGETFPLFKKGKLNHVFIIAGGSFVDEKILP"
     gene            complement(2270..>2703)
                     /locus_tag="PKH10_10945"
     CDS             complement(2270..>2703)
                     /locus_tag="PKH10_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNT28677.1"
                     /translation="KKKYNLQVGPKSAEQIKCIIGNAFPDEKRTFMEVKGLDLVAGIP
                     KTIEMNSEEIRESLADCVGGIVQAVRSVLEKTPPQLAADIVDKGIVLAGGGALLKNLD
                     ILLREETNLPVIIADDPLKSVVVGIGRILENIDLLQTVSLT"
BASE COUNT          611 a          757 c          821 g          514 t
ORIGIN      
        1 gttggtgagg ggacgcagga cgctttcgga gtattcgagc cacacgctgg gcgatatgcg
       61 cctgcccgac agcagcgtgg ggtcgtaact cgggttggaa tagagcgcga ggatgtcgcc
      121 ggtatggata tccatcatga ccaccgcccc agcgcgctca ccgagcgccg cctccgcctc
      181 ttcctgcaga taggaaagga cggctgtctg caccgatcgc cccttcctgg ccggcaccgt
      241 cggcggcacc tctccggagg atcggtcctg cttctcgatg cggcggccga catggtcgat
      301 cacctgccac aattcgccgt acacgccgtg aagttcacgc tcccacgaac gctcgatgcc
      361 ggtcttgccg acgaaacttt ccggatcgta cccggggtat gctgagagat cgccgcggga
      421 tatgcgcccg acatagccgg tcaggtgggc cgcagtgatg cccagtggat atacacgtcg
      481 atacgaggag gatacagcga cgccgggcac atcggtcaga taactcgaca ggcggagcat
      541 ctcgcttttg ggcagttcat ccctgaccgt gaccggcgta taacggcatc ggccgcggca
      601 ggtcgaaagg aactcgacca attcgccgac ctgccgatcg gaaagtccga gtatcgaggc
      661 aaggcgcatg ctgctctcct caacctcatc gcctgtgaag aaagcgggca gtatctggat
      721 gcggaactgc gccgatgtgg tcgcgagtgt ccgaccgtcg gcggtaagta tgtcgccgcg
      781 cgccgggaat atcttctcgc gcaggagata gttgttcttc gatttctgca ggtgctcccc
      841 gccacggtac agttggagat accctatccg caccgaaagg agaagaagcg agatgaagat
      901 gacgcccagc attacccgcg acttgcgggc gatattgcgg acctcctcct gctggtcgag
      961 tatcctcata gccacgcatc ccgggtatag agcgacacga tcgggcggcg acggaacagg
     1021 aagaggaata gcagtaccgc gaggttgacc gtggccgccg caagcccgtc cgcgagtccc
     1081 gcaagaccgg tgaagaggaa acgatagatc atgacatcgg ctatcacgag cagtgatgag
     1141 agaaaggtcg tcaggaacag gaccaaggag aactggaagg tggtcacctc gagcgtgagt
     1201 cgcgtgaaca catacaggtg cccgaagagg aacgcgatga agaacagggg aagatcgtga
     1261 gccaacagaa cgaacagcac gccgttcagc aggaacgata ccatccgcat ccgctccttc
     1321 ttttcgtagc acaggaaggt gaagaggaat ggcagatgcg ggaagtagtg aaagggaagg
     1381 tcgaacaggt acaggaagaa cgagaagagc gcgaacaagg ccgcgaggcc ggcatggaat
     1441 tcaagggaga atcttttcat cgacgaacga tcctccggcg atgatgaata catgattcaa
     1501 tttccctttt ttaaaaagag ggaatgtctc tccgcgatag aaaaggatat tgttctcgtc
     1561 ctcgataagc gaaccgatga ccccgatggg gatatagggc aaactgtagt tctcgctgag
     1621 attcgccgag agcatcttgc gaccgacggc cgacggctcg ttacagacct tctcgaagaa
     1681 ctggatcgaa agcgtctgac cgttcccgcg gagtatcgcg gtacattcct ctcgtgactc
     1741 aagaatggcg ggaagactga aatgacgcga ggtgataaga tcgacctcgg cggaattcgc
     1801 cccgaacgac ctgacgagcc ccacgagccc catcgaggag accaccggtg cgtttaacgc
     1861 gaggtgcttg gcgaactccc cgtcgtaatg tatcgtgatg cgggtgggat tgacggatga
     1921 ctgactgaga aggcgggccg gtatgatggt ataaccgcta taacggtcct ttatcttgag
     1981 cagtttcgcc agttcttcgt tctcgcgctt tatcccctcg ctcatcatga tctggagccg
     2041 aagccgtgcg ttctcctcct cgagcgcgat gttccgttcg ccgaccgtat ggaggtcgcg
     2101 caggatggcg accgatgtga aaagacggga cacccgttcg ctgacggcga tcctggcatc
     2161 acgaacgatc ggtgcacgcc acgaccattc ggtcaggagc cggggcaaaa gcgccaaagc
     2221 gaacacgaac acggcgaaca gcgcgatgag caggtatcgc ttccgcacct caggtcagcg
     2281 aaacggtctg taaaaggtca atgttctcta gtatccgccc gatgccgacg acgaccgatt
     2341 tgaggggatc gtcggctatg atgacaggca ggttggtctc ctcgcgcaag aggatgtcga
     2401 gattcttgag gagcgcaccg ccgccggcaa gcacgatccc cttatccact atgtcggcgg
     2461 caagctgcgg cggggtcttc tcgagcaccg accggaccgc ctgcacaatg ccacctacgc
     2521 agtcggcgag gctctcgcgg atctcctcag agttcatctc gatggtcttg ggtatgccgg
     2581 cgaccaggtc gagccccttg acctccatga aggtcctttt ctcgtcggga aaggcattac
     2641 cgatgatgca ttttatctgc tcggccgact tcggaccgac ttgtagattg tactttttct
     2701 tga
//
LOCUS       DAOQUE010000398         2685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_45334_length_2685_cov_4.112167, whole genome shotgun sequence.
ACCESSION   DAOQUE010000398 DAOQUE010000000
VERSION     DAOQUE010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45334_length_2685_cov_4.112167"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="PKH10_10950"
     CDS             <1..1269
                     /locus_tag="PKH10_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HNT28678.1"
                     /translation="SMTRNRDNQLYLALTPTAPARCGSSPGQVTVDYLETVISYRIGE
                     PTVTAFDKMDYYISTSTISTGWQGARNACLDRGMDLVVINSEAEQQFLATLSGYSSSA
                     YYWIGLNDNLKADDWRWVSGDLIWKGNYSSGRAFDGSFNYWYSASYPSSSTSNCANFG
                     ASSYSGEWYNTSCTSTLRRYICEKPRNKFHHQFDATALDRGTAEAACIDIGGHLSILD
                     NELDQMSIEKYQTTNQNYWIGLDDLTTEGTFVWENGVTAWSGTVSGLPYGYTNWSDAQ
                     PDGNGDGVIASGTDNFRWGDVDETGTNYYTCEMTDTNCARNNGFCDDRDDCCSGTCHM
                     ASVTCVACINTEETSCTTSAGCCNALDTCSSGKCCRNTGVSCTSASQCCSTSHLCQGG
                     LCCYKTGARCTVNSQCCSGLCSGVFPKYCQ"
     gene            complement(1314..>2685)
                     /gene="rimO"
                     /locus_tag="PKH10_10955"
     CDS             complement(1314..>2685)
                     /gene="rimO"
                     /locus_tag="PKH10_10955"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNT28679.1"
                     /translation="RVATRAAPISCPRRGSRNTDPVKNNRLYLESLGCPKNRVDSEVM
                     LAALIADGWEVIDDPARADLIILNTCAFVEDARRETIERFFDLHRRRSKGARLAVAGC
                     LPQLVPDIAKSLVEADIVLGLNDIPRVAELVREKRGASRRIAAPEFLMTARQERVATL
                     SPHTAYLKIADGCDNRCAYCAIPLIRGVYRERSPRDILEEARRLIDGGVREIVLIAQD
                     TTAYGRDHDTDLATLLRRLNRLPGEFLIRSLYLYPSGIDGRLLDAFGGEKVIPYFEIP
                     LQHVADRVLAAMGRRYRRADIERVTDSVLARFGERAILRTTLIAGFPGETAREFDEMQ
                     RFTADAPFDYVGVFGYSKEQGTPAGRLRSLPRRVIEERTALLRKTAETAMERRLDRFV
                     GMRTTVLYEGFDADSCLATGRALQQAPEIDGITILTNIDRERPGDLLPARIVARDGVD
                     LIAEAL"
BASE COUNT          506 a          852 c          807 g          520 t
ORIGIN      
        1 tcgatgaccc gcaatcgcga caatcagtta tacctcgccc tgacgccgac cgcccccgcc
       61 cggtgcggca gttcgccggg acaggtgacc gtcgattacc tcgagacggt catctcctat
      121 cgcatcggcg agccgaccgt cacggcgttc gacaagatgg attactatat ctcgacctcc
      181 accatttcca ccggttggca gggggcccgt aatgcctgtc tcgatcgcgg tatggatctg
      241 gtcgtcatca actccgaggc cgaacagcag ttcctcgcga ccttgagcgg ttattcatcc
      301 tctgcttact actggatcgg gctcaacgac aaccttaaag ccgacgactg gcgctgggtc
      361 tcgggcgacc tcatctggaa aggcaactac tcctctggca gagccttcga cggtagtttc
      421 aactactggt atagtgcctc ctacccctct tcttcgacat cgaattgcgc gaatttcggg
      481 gcaagctcct attcgggaga atggtacaac acctcctgca cctccacgct acggcgttac
      541 atatgtgaaa aaccgcgcaa caagttccac catcagttcg atgctacggc cctcgatcgg
      601 ggaacggccg aggccgcttg tatcgatatc ggcggtcacc tctcgatact cgacaacgaa
      661 ctggaccaga tgtcgataga aaagtaccag acgaccaacc agaattactg gatcgggctg
      721 gacgatctga cgaccgaggg aacctttgtc tgggagaacg gcgtcaccgc atggtcgggc
      781 accgtgagcg ggcttcccta cggatatacc aactggtctg acgcacagcc cgacgggaac
      841 ggggatggag tgatcgcaag cggtactgat aatttccgtt ggggagatgt cgacgagacg
      901 ggcaccaact actacacctg cgagatgacc gataccaact gcgcccgcaa caacggtttc
      961 tgcgatgacc gggacgactg ctgttccgga acctgccata tggcgagcgt cacctgtgtc
     1021 gcctgcatca acaccgaaga gacctcctgc accacctcgg ccggttgctg taacgcgctc
     1081 gacacctgtt ctagcgggaa gtgttgtcgt aacaccggcg tctcctgtac ctcggcgagc
     1141 cagtgctgtt cgaccagtca tctgtgccaa gggggtctct gttgttataa aaccggcgct
     1201 cgttgtaccg ttaattcaca gtgttgcagc gggctctgta gcggggtctt cccgaaatac
     1261 tgtcagtagc caacgccacg catctccgcc ttctcatatc acatctgcga gttctaaaga
     1321 gcctcggcga tgagatccac gccgtcgcgg gcgacgatgc gcgccggaag aaggtcaccg
     1381 ggtcgttcgc ggtcgatgtt ggtgagtatc gtgatgccgt ctatctccgg cgcctgttga
     1441 agcgcccgcc ccgtcgcgag gcaactgtcc gcgtcgaacc cctcgtacag caccgtggtg
     1501 cgcataccga cgaaccggtc gagccgccgt tccatcgccg tctccgccgt ttttcgtaga
     1561 agtgcggtcc gctcctcgat gacgcgccgc gggagcgacc gcaggcgtcc cgccggggtc
     1621 ccctgctcct tcgaatagcc gaacacgccg acatagtcga acggcgcatc ggccgtgaac
     1681 cgttgcatct cgtcgaactc ccgcgccgtc tcgcccggga agccggcgat gagcgtggtc
     1741 cgcaggatgg cccgttcgcc gaaccgcgcg aggactgagt ccgtcacccg ttcgatgtcg
     1801 gcgcgccggt agcggcgccc catcgctgcg agcacgcggt cggcgacatg ctggagcggt
     1861 atctcgaaat acgggatcac cttctccccg ccgaatgcgt caagaagccg gccgtcgatg
     1921 ccggacgggt agaggtagag tgatcggatg aggaactcgc ccggcagacg gttcaatctt
     1981 ctcagaagcg tggcgaggtc cgtatcgtgg tcacgaccgt aggcggtggt atcctgcgcg
     2041 atgaggacga tctcccggac gcccccgtcg atgagccgcc gcgcctcctc gagtatgtcg
     2101 cgcggcgacc gttcacggta gacgccgcgg atgagcggga tggcgcagta ggcacaccgg
     2161 ttatcgcacc cgtcggctat cttgaggtag gcggtgtgcg gcgacagcgt ggcgacccgc
     2221 tcctgtcgcg ccgtcatgag gaactcgggc gccgctatgc gccgggacgc gccgcgcttt
     2281 tccctgacca gttctgcgac acgcggtatg tcgttcagtc cgagcacgat atcggcctcg
     2341 acgagactct tcgcgatatc ggggaccagc tggggcagac aaccggccac ggcaagccgc
     2401 gcacctttac tgcgtcgccg gtgcagatcg aagaaccgct ctatggtctc gcgtcgggca
     2461 tcctcgacaa aggcgcaggt gttgaggatg atgagatcgg cgcgcgccgg gtcgtcgatg
     2521 acctcccagc cgtcggcgat gagcgccgcg agcatcacct cgctgtcgac gcgattctta
     2581 ggacagccga gcgattcgag gtagaggcgg ttgttcttca cgggatcagt atttcttgag
     2641 ccgcgcctcg gacacgagat aggcgccgct ctcgtcgcga ccctc
//
LOCUS       DAOQUE010000399         2682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_45405_length_2682_cov_4.488390, whole genome shotgun sequence.
ACCESSION   DAOQUE010000399 DAOQUE010000000
VERSION     DAOQUE010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45405_length_2682_cov_4.488390"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..586
                     /locus_tag="PKH10_10960"
     CDS             161..586
                     /locus_tag="PKH10_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HNT28680.1"
                     /translation="MISFDPTLFLLQALLFPVFLFLFGRWVFDPIQGVLAERDRRLNR
                     EGTHADEASRTLAELKERYRREMETVHRAGMALVAEAKERAEREARTTVEKVSDEMHE
                     MVAAEQEAARQQCGVIFDEIRSHRGDLEEAIADKLWGRP"
     gene            583..1068
                     /locus_tag="PKH10_10965"
     CDS             583..1068
                     /locus_tag="PKH10_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28681.1"
                     /translation="MSATAFFIIALINFALFAAILLRLARRPVMSLFAERERTTRETM
                     ERYRDAYERAEQEIARFRRLLEDLPEERRRVTERYGERAAQAAAEIADRARRETDFLR
                     REAHRAAREARDRCYEESFASLVAALVTDAERGAGALDAERRRAVLASSTALLERGGA
                     R"
     gene            1065..1613
                     /gene="atpH"
                     /locus_tag="PKH10_10970"
     CDS             1065..1613
                     /gene="atpH"
                     /locus_tag="PKH10_10970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNT28682.1"
                     /translation="MSDPATRYAQALLDGVKGPASAATALFGHGRDLVELLAAEDDLR
                     RFLESPAFGRDEKRELLAVVGARCGYESALLNLCGALVDNHRIGLLATVLDRAERLWR
                     RRQGIAELEVSVARPLADDEESDLRRVLAERFGKTAEARFTVDPAVLEGLVVKKDSVC
                     YDASLRNHIRLLRDRLREGKGR"
     gene            1632..>2682
                     /gene="atpA"
                     /locus_tag="PKH10_10975"
     CDS             1632..>2682
                     /gene="atpA"
                     /locus_tag="PKH10_10975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNT28683.1"
                     /translation="MQGTVLRGGEIGKILRERIEQFEKRLETREVGSVISASDGIARV
                     YGLDRAFAGEIVEFASGANGMVLNLEPDSVGVAVMGDHEHIREGEEVRRTGRIMSVPV
                     GDAMVGRVIGALGEPLDGLGPIDTSAYRPVEVKAPGIVKRQPVKEPLQTGLKAVDAMI
                     PIGRGQRELIIGDRQTGKTALAIDAIINQKGGDVLCVYVAIGQKRSTVAQVVDKLRRH
                     GAMDYSIVIAATASDPAPLQFIVPYAGATIGEHFRDSGRHALIVYDDLSKHAVAYRQL
                     SLLLRRPPGREAYPGDIFYLHSRLLERAAKLSESAGGGSLTAFPVIETQAGDISAYIP
                     TNVISITDGQIFLESD"
BASE COUNT          462 a          864 c          936 g          420 t
ORIGIN      
        1 aggaatggca tgtctcctac acgctccgct cgacctgcga aggcccgctc tacaccgcgc
       61 tcaaacgact cgccgaccgg cagttcgtga agccctcctc ccagcagtcg cggctcattc
      121 tcgagccgtc gtcagacggg aactatacgg tccgcatccc atgatatcgt tcgacccgac
      181 cctttttctc ctgcaggcgc tcctgttccc ggtgttcctg ttcctgttcg gccgctgggt
      241 gttcgacccg atacagggcg tgctcgccga gagggaccgc cggctcaacc gggaggggac
      301 gcacgccgac gaggcgtcac ggacgctcgc cgaactgaag gaacgatatc gccgggagat
      361 ggaaacggtc caccgcgccg ggatggcgct cgtcgccgag gcgaaggaac gggccgagcg
      421 cgaggcgcgc accacggtcg aaaaggtatc tgatgagatg cacgagatgg tcgccgccga
      481 gcaggaagcg gcccgacagc agtgtggtgt gatattcgac gagatacggt cgcaccgcgg
      541 cgacctcgag gaggcgatag cggataagct gtgggggcgt ccgtgagcgc caccgccttt
      601 ttcatcatcg cgctcatcaa tttcgctctg ttcgcggcca tcctcctccg gctggcgcgt
      661 cgaccggtca tgtcgctttt cgccgagcgc gaacggacga cgcgcgagac gatggaacgg
      721 taccgcgatg cctatgaacg ggcggaacag gagatagcgc ggttccgccg tctgctcgaa
      781 gacctgccgg aggagcggcg gcgcgtgacg gagcggtacg gggagcgggc ggcgcaggcc
      841 gccgccgaga tcgccgatcg cgcgcgtcgc gagaccgatt ttctccggcg cgaggcgcac
      901 cgcgccgcgc gcgaggcgcg cgaccggtgt tacgaggaat cgttcgcgtc gctcgtcgcc
      961 gcgctcgtca ccgacgcgga acggggcgcc ggcgcgctcg acgccgaacg gcgccgcgcc
     1021 gtgctcgcgt cgtcgacagc gcttctggaa cggggaggcg cgaggtgagc gatcccgcga
     1081 cccggtacgc gcaggcgctg ctcgacggcg tgaaggggcc cgcctccgcc gcgacggccc
     1141 tgttcgggca cgggcgcgat ctggtggaac tgctcgccgc ggaggatgac ctgcgccgtt
     1201 tcctcgagtc gccggcgttc ggccgcgacg aaaagcggga actgctcgcg gtggtcgggg
     1261 cgcggtgtgg ctacgagtcg gcgcttctca acctctgcgg agcgcttgtc gacaaccatc
     1321 gtatcggtct gctcgcgacg gtgctcgata gagccgaacg gctctggcgc cggcgacagg
     1381 gcatcgccga actggaggtg tcggtcgccc ggccgcttgc cgacgacgag gagtccgacc
     1441 tgcgccgcgt cctcgcggag cgtttcggaa aaacggccga ggcgcgcttc acggtggatc
     1501 ccgcggtgct cgaagggctg gtggtaaaga aggattcggt ctgttatgac gcgtcgctcc
     1561 ggaaccatat ccggttgttg cgcgaccggt tgcgcgaagg aaaaggacga taggatacgg
     1621 gagagacgac catgcaggga acggtgctca ggggcgggga gatcggcaag atactccgcg
     1681 aaaggataga acagttcgag aaacggctgg aaacgcgcga ggtcggatcg gtcatctcgg
     1741 cgagcgacgg catcgcgcgt gtctacgggc tcgatcgcgc gttcgccggc gagatcgtcg
     1801 agttcgcgag cggcgcgaac ggcatggtgc tcaatctcga accggactcg gtcggcgtcg
     1861 ccgtcatggg cgaccacgag cacatccgcg agggcgagga ggtgcggcgt accggccgca
     1921 tcatgtcggt gccggtcggt gacgcgatgg tggggcgcgt gatcggcgcg ctcggcgaac
     1981 cgctggacgg gctcggcccc atcgacacct cggcctaccg tcccgtcgag gtgaaggcgc
     2041 ccggcatcgt gaagcgccag ccggtcaagg agccgctcca gaccgggctc aaggcggtgg
     2101 atgcgatgat acccatcggc cgcgggcagc gcgaactcat catcggcgat cgtcaaaccg
     2161 gcaagacggc gcttgccata gatgccataa tcaaccagaa ggggggcgat gtactctgcg
     2221 tctatgtcgc catcggccag aaacggtcga ccgtcgcgca ggtggtcgac aaactgcggc
     2281 gacacggcgc gatggactac tccatcgtga tcgccgcgac ggcgagcgac ccggcgccgc
     2341 tccagttcat cgtgccctac gcgggggcga ccatcggcga acatttccgc gattcgggcc
     2401 gccatgcgct catcgtgtac gacgatcttt ccaaacacgc ggtcgcctac cggcaactgt
     2461 cgcttctcct acggcggccg ccgggccgcg aggcgtaccc gggcgacata ttctacctcc
     2521 actcgcgcct gctcgaacgg gccgcgaaac tgtcggagtc cgcgggcggc ggatcgctca
     2581 ccgcgttccc cgtcatcgag acgcaggcgg gcgacatatc ggcctatatc ccgaccaatg
     2641 tcatttcgat aaccgacggc cagatattcc tcgaaagcga tc
//
LOCUS       DAOQUE010000400         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_45687_length_2672_cov_4.439434, whole genome shotgun sequence.
ACCESSION   DAOQUE010000400 DAOQUE010000000
VERSION     DAOQUE010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45687_length_2672_cov_4.439434"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..83)
                     /gene="tsf"
                     /locus_tag="PKH10_10980"
     CDS             complement(<1..83)
                     /gene="tsf"
                     /locus_tag="PKH10_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707555.1"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HNT28684.1"
                     /translation="MDISASQVKELRERTGAGILDCKNALV"
     gene            complement(121..1011)
                     /gene="rpsB"
                     /locus_tag="PKH10_10985"
     CDS             complement(121..1011)
                     /gene="rpsB"
                     /locus_tag="PKH10_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNT28685.1"
                     /translation="MVVDIRELLKAGAHFGHQKQKWNPKMAPYIFTERNGVHIINLEK
                     TVGLIESAAKFLKNLGLNGDPVLLVGTKRQAQEVIKQAAERAGAPYVTNRWLGGMLTN
                     FQTIGQSIKRLKKYDEMLSEEKRYQYTKKELITIAKEKTKLEKNLSGIVRMSKLPGAV
                     FVVDPRREHLAVHEATKLGIPVIAIIDTNGNPDPIDFPLPANDDGMRAIDIIVNGLVD
                     AYLEGFDIHRQKAQTETKDHRDRAGRGDETRNVAGRKVKVKRIGGDEGEAKATGDGDA
                     KKPVRRTAPKKDEAAGEAAK"
     gene            1221..>2672
                     /locus_tag="PKH10_10990"
     CDS             1221..>2672
                     /locus_tag="PKH10_10990"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28686.1"
                     /translation="MSERTTSFIETAPEAVPVLVAGLARQHRRLVIEAPGPREAALLA
                     RSLHYFDVDCELFLPPFLLMERGAYDASWEQRLIRLLEKSRGIVVVTPLSRRFRLPAA
                     LDAGALRIAPGTRLTATGLSRDLAARGYDPVPIVRGFGEFSVRGDIIDIAAYEPDTGW
                     RIELFDDEVEKISRFSLTSQRNTGDDPEAHLFPRIVSRLLAPDWKARLTSLLAAEGNR
                     RIMEVEEAIEAGAGLPGDLHPLTAGDRLIEEYFDAPVIRWERVRCEMALDEEMKPLTT
                     ERERRRAEGAFTPFDIRASFRERAAAPIVEISSFFSTAEAIVKYPVAHQQASPHEKEH
                     PELLLGRLAADNAVVLFTDHGGRDRWGDLCVEKDIPSIPLETLPSAPERGTVHIIEGD
                     GWAAPDAVLIVPHAGLVVLHEKLFAVRAPAKPSRRRAVEEAPEADETRQPLLLDELIP
                     GAPVVHYTYGVAVYEGMRRIEKTDCLTLRYDHDD"
BASE COUNT          432 a          911 c          800 g          529 t
ORIGIN      
        1 tcgacaagcg cgttcttgca gtcgagtatg ccggcgccgg tccgttctct cagttctttg
       61 acctgtgacg cggagatatc catgatcatc gttcctcctt gctgatggtg acgctgttcc
      121 ttacttggcg gcctctcccg ccgcctcgtc ctttttgggg gccgtccggc ggaccggctt
      181 tttggcatcg ccgtcacccg tcgccttcgc ctcgccctca tcgccgccga tcctcttgac
      241 cttcacctta cgaccggcca cattccgcgt ctcgtcgccg cgcccggcgc gatcgcggtg
      301 atccttggtc tcggtctgcg ccttctggcg atggatgtcg aacccttcga gatacgcgtc
      361 cacgagcccg ttgacgatga tatctatcgc ccgcatgccg tcgtcgttgg ccggcagggg
      421 gaaatctatc ggatcgggat tgccgttggt atcgatgatc gcgatgaccg ggatgcccag
      481 tttggtcgcc tcatgcaccg cgagatgttc gcggcgggga tcgacgacga agacggcgcc
      541 cggcaatttc gacatcctga cgatgcccga aaggttcttt tcgagtttcg tcttctcctt
      601 ggctatggtg ataagttcct ttttggtgta ctggtagcgc ttctcctcgg aaagcatctc
      661 atcgtacttc ttcagccgtt ttatcgattg acctatcgtc tggaagttgg tcagcatgcc
      721 accgagccac cggttggtca catagggggc gccggcccgt tcggccgcct gcttgatgac
      781 ctcctgcgcc tgtcgtttgg tgccgacgag caggaccggg tcgccgttca gcccgaggtt
      841 cttgaggaac ttcgccgccg attcgatgag cccgaccgtc ttttccagat tgatgatgtg
      901 cacgccgttg cgttcggtga agatgtacgg cgccatcttg gggttccatt tctgtttctg
      961 atgcccgaaa tgggcgcccg ccttcaacaa ttctctgatg tccactacca tgtgatgcct
     1021 ccaaaaaaag agttacctgt tatcgcttcc cccggccctt ccgctcaccg ttccgcggcc
     1081 cgcgcggaac ggacaggtac ggcgggcctg tcccggggtg cttttaacag cggcatagat
     1141 actctgaaac ccctgtttgt caaccttttt ctggcaaaat cggcccggct cgctatcatc
     1201 acagcgacca cgaggagacc atgagcgaac ggacgacctc tttcatcgaa acggcccccg
     1261 aagcggtacc ggtgctcgtc gcgggtcttg cgcggcaaca ccgccggctc gtgatcgagg
     1321 cgccgggacc gcgcgaggcg gccctgctcg cccgctcgct ccactatttc gatgtcgact
     1381 gcgaactgtt cctgccgccc ttcctgctca tggaacgcgg tgcatacgac gcctcatggg
     1441 aacagcggct catccgcctg ctggaaaaga gccgtggcat cgtcgtggtc accccgctct
     1501 cgcgacgatt cagactgccc gccgcgctcg acgccggcgc gctccgcatc gcgcctggca
     1561 cccggctgac cgccaccggg ctttcacgcg accttgccgc acggggttac gacccggtcc
     1621 ccatcgtccg gggcttcggg gaattctcgg tgcgcggcga catcatcgac atcgccgcct
     1681 acgagcccga caccggctgg cgcatcgaac tgttcgacga cgaggtcgag aagatcagcc
     1741 gcttctcgct caccagccag cgcaacaccg gcgacgaccc cgaggcgcac ctgttccccc
     1801 gcatcgtctc acgactgctc gcccccgact ggaaggcgcg cctcacctcg ctgctcgccg
     1861 ccgaagggaa ccggcgcatc atggaggtcg aggaagcgat cgaggcggga gccgggctcc
     1921 ccggcgacct tcacccgctc acggcgggcg accggctcat cgaggagtat tttgacgcgc
     1981 cggtcatacg ctgggaacgg gtccggtgcg agatggcgct cgacgaggag atgaaaccgc
     2041 tcacgaccga acgggaacga cgccgcgccg aaggggcctt cacgccgttc gatatacgcg
     2101 cctcgttccg cgaacgggcc gccgcgccga tcgtcgagat aagttccttc ttctcgaccg
     2161 ccgaggccat cgtcaaatat ccggtcgccc accagcaggc gtcaccgcac gagaaggaac
     2221 atcctgaact gctcctcggg cggctcgcgg ccgacaacgc cgtcgtactc ttcaccgatc
     2281 acggcggcag ggaccgttgg ggcgacctgt gcgtcgaaaa ggatatcccg tccatcccgc
     2341 ttgaaacgct tccttccgcg ccggagcgcg gcaccgtcca catcatcgag ggggacggct
     2401 gggccgcgcc cgacgcggtg ctcatcgtgc cgcacgccgg gctcgtggtc ctgcacgaga
     2461 aactgttcgc ggtgcgcgcc ccggccaagc cgtcgcgccg acgggccgtc gaggaggcgc
     2521 ccgaggcgga cgaaacgcgt cagccgctct tgctggacga actgataccg ggcgccccgg
     2581 tcgtccatta cacctacggc gtcgcggtgt acgaagggat gaggcggatc gagaagaccg
     2641 actgtctgac gctccggtac gaccacgacg ac
//
LOCUS       DAOQUE010000401         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_46030_length_2659_cov_3.749616, whole genome shotgun sequence.
ACCESSION   DAOQUE010000401 DAOQUE010000000
VERSION     DAOQUE010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46030_length_2659_cov_3.749616"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PKH10_10995"
     CDS             complement(<1..588)
                     /locus_tag="PKH10_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HNT28687.1"
                     /translation="MIRKFAALLLLAGIAAIFMGLLFTYEPDPELNTTARYYAERTAA
                     DIGAANIVTAIVVTYRGLDTLGEVTVLFLSAAIVGLVLASGRRKKTAKERTVTPAGEL
                     LATGSTLLAPLIMLLGVYVFVNGHLTPGGGFQGGAIIASGMLLLLLTDPLKHFSHTLI
                     ATVESLSGILYVAIGLLGIFLAGGFLDNRILPIGTL"
     gene            complement(581..835)
                     /locus_tag="PKH10_11000"
     CDS             complement(581..835)
                     /locus_tag="PKH10_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HNT28688.1"
                     /translation="MSALFIALTILLTVAMIGAALVAVLGKSMPIAILSAGLVSLLAS
                     VLFLLLAAPDVAMTEAAIGSGLTTFLFFFILGRVRGGEHD"
     gene            complement(832..1164)
                     /gene="mnhG"
                     /locus_tag="PKH10_11005"
     CDS             complement(832..1164)
                     /gene="mnhG"
                     /locus_tag="PKH10_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HNT28689.1"
                     /translation="MTIGGIIGGILMLSGAAFLFLAGLGLVRMPDLFNRLQAGTKATT
                     LGTLLTLAGAACLRPDWAVKLLLIGLFILFTNPISSQVLARAAHRTGTPQAAGTVDRL
                     AEDREKRP"
     gene            complement(1166..1426)
                     /locus_tag="PKH10_11010"
     CDS             complement(1166..1426)
                     /locus_tag="PKH10_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015850181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HNT28690.1"
                     /translation="MVDILIYIAAAVTGIAFLFALYRFLKGPTAADRVVAFDTMTLVT
                     VVFIVIIALAEGRGIYLDVAVIYALLSFLGVIAVARYLERGL"
     gene            complement(1419..1916)
                     /locus_tag="PKH10_11015"
     CDS             complement(1419..1916)
                     /locus_tag="PKH10_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNT28691.1"
                     /translation="MKKASAIAILFLTLLIFWLLLNGSLAADIVIVGVVTALVIAILF
                     RDGLAVFSEFRATPRAFLTAFLYLPYFLGELIKSNLHIARVVLTPSLPLNPAIVKVRT
                     RLKSRMGRLILANSITLTPGTLTVELDGEWLYIHWVTAEATDIDAATAQIVAGFEKYL
                     EVMYG"
     gene            complement(2063..>2659)
                     /locus_tag="PKH10_11020"
     CDS             complement(2063..>2659)
                     /locus_tag="PKH10_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HNT28692.1"
                     /translation="SGLSPYLHFGQISAQAAALAIRDYPAPTAAVEAFLEQLIVRREL
                     ADNFCLYQPHYDSFDGIPAWAQKTLNDHRADKRLYRYTSTTWEQATTHDDLWNAAQRE
                     LIATGAMHGYLRMYWAKKLLEWSATPEEAIATAVYLNDRYALDGRDPNGYAGILWSIG
                     GLHDRPWFVRPIFGAVRYMGGAAFRKKPGMRGHIKKWS"
BASE COUNT          545 a          751 c          932 g          431 t
ORIGIN      
        1 cagcgtgccg atgggcagta tccggttatc gaggaagccg cccgcgagga atatccccag
       61 caggccgatg gcgacataga ggatgcccga gagcgattcg acggtggcaa tgagcgtatg
      121 gctgaagtgt ttcagcggat cggtgaggag aagcagcagc atccccgatg cgatgatcgc
      181 gcccccctga aaaccgccgc ccggggtgag gtggccgttc acgaagacat agacgcccag
      241 cagcatgatg agcggcgcga ggagggtgct gccggtcgcc agcagttcgc ccgccggggt
      301 cacggtccgc tccttggcgg tcttcttccg gcgacccgac gcgaggacga gccccacgat
      361 ggcggccgaa aggaacagca ccgtcacctc gcccagcgtg tcgagtccgc ggtaggtgac
      421 aacgatggcg gtgacgatgt tggccgcgcc gatgtcggcg gcggtccgtt cggcgtagta
      481 gcgggcggtg gtattgagtt cggggtcggg ttcgtaggtg aaaaggagtc ccatgaagat
      541 cgcggcgatg ccggcgagga gcagaagcgc ggcgaacttt ctaatcatgc tcgcctcccc
      601 gcacccgtcc gaggatgaag aagaacagga aggtggtaag cccgctcccg atggccgctt
      661 cggtcatcgc gacgtcgggt gcggcgagga ggaggaacag gaccgacgcg agcagactca
      721 cgagccctgc ggagagtatc gcgatgggca tgctcttccc gagcaccgcg acgagggcgg
      781 cgccgatcat cgcgacggtg agcagtatgg tcagggcgat gaagagcgcg ctcacggccg
      841 cttctcccgg tcctcggcga gccggtcgac cgttccggca gcctgcgggg tgccggtccg
      901 gtgcgccgcg cgggcgagca cctgtgacga tatcgggttg gtgaagagga tgaacagacc
      961 gatgagcagc agtttgaccg cccagtcggg gcggaggcag gcggcgccgg cgagcgtgag
     1021 gagcgtcccg agcgtcgtcg ccttggtccc cgcctgcagg cggttgaaga gatcgggcat
     1081 tctaacgagc ccgagccccg cgaggaacag gaacgccgcg ccggaaagca tgaggatgcc
     1141 gccgatgatc ccgccgatcg tcatctcaca gcccccgttc cagatagcgg gccactgcga
     1201 tcacgccgag gaaggagaga agcgcgtaga tgaccgcgac atcgagatag atgccgcgtc
     1261 cttcggcgag cgcgatgata acgatgaata cgaccgtgac gagcgtcatg gtgtcgaacg
     1321 cgaccacgcg gtcggccgcc gtcgggccct tgagaaaacg gtagagggcg aacaggaagg
     1381 cgatccccgt gaccgccgcc gctatataaa taaggatatc aaccatacat gacctcgagg
     1441 tacttctcga aaccggccac gatctgcgcc gtcgccgcat cgatgtcggt cgcctcggcg
     1501 gtgacccagt ggatatagag ccattcgccg tcgagttcga ccgtgagcgt cccgggggtg
     1561 agcgttatcg aattggcgag gatgagccgc cccatgcgac ttttgagacg cgtccgcacc
     1621 ttgacgatgg cggggttgag cggcagggat ggggtgagga cgacgcgggc gatatgaaga
     1681 ttcgacttga tcagttcgcc gaggaagtag gggaggtaga ggaacgccgt gagaaaggcg
     1741 cgcggggtgg cgcggaattc ggagaagacc gccagcccgt cacggaaaag tatcgcgatg
     1801 acgagcgccg tgacgacgcc gacgatgacg atgtcggccg cgagtgagcc attgagaagc
     1861 agccagaaga taagcagcgt caggaacaat atcgctatcg ccgaggcttt cttcattaaa
     1921 ccaccatcat cgaggacgcg caagtataac aaagcggaac gcgaaggcaa agaaaaagca
     1981 tcacacgcgg tcccgtttaa aggttgccac tagcaacaat gttataggga acagaaaatg
     2041 aaagtcaagg gaaaagggat actcaactcc acttttttat atgcccccgc atccccggtt
     2101 ttttgcggaa ggcggcgccg cccatgtagc ggaccgcgcc gaagatgggg cgtacgaacc
     2161 acgggcggtc gtggaggccg ccgatgctcc agaggatgcc ggcgtaaccg ttggggtcac
     2221 ggccgtcgag ggcgtagcgg tcgttgaggt acaccgccgt cgcgatcgcc tcctcggggg
     2281 tcgcgctcca ttcgagcaac ttcttcgccc agtacatcct gaggtagccg tgcatcgcgc
     2341 cggtcgcgat gagttcccgc tgggcggcgt tccagaggtc gtcatgggtc gttgcctgtt
     2401 cccatgtagt gctggtgtag cggtagagcc gtttatcggc gcggtgatcg ttcagcgttt
     2461 tctgcgccca cgccgggatg ccgtcgaacg agtcatagtg cggttggtag aggcagaaat
     2521 tatcggcgag ttcgcgccgg acgatgagtt gttcgaggaa cgcctcgacc gccgctgtcg
     2581 gcgccgggta gtcgcggatg gcgagcgccg ccgcctgcgc cgatatctga ccgaagtgca
     2641 ggtaggggga tagaccgga
//
LOCUS       DAOQUE010000402         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_46055_length_2658_cov_3.753746, whole genome shotgun sequence.
ACCESSION   DAOQUE010000402 DAOQUE010000000
VERSION     DAOQUE010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46055_length_2658_cov_3.753746"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="PKH10_11025"
     CDS             complement(<1..1194)
                     /locus_tag="PKH10_11025"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNT28693.1"
                     /translation="MDRLKGELKKIMGRFPGYGFRFRYLDWETDFLRFYRSQANYNIS
                     KRTVVLNGTVEREKRKFSFQIADPDGAKASAMVERAVGLLNDLPPDPDFTSFEDDPTQ
                     YEYRDFLKAADSAPLSRKLDILEQATAAAGPLGFDIYGTFITVSSDGFLLQSNGLDKR
                     IFVSPLMLDMKAVARSNGVTVINSFGGTSLDPFDPAAFMADLARKMKWATLPVTDVEP
                     GEYTVILGPQAVHTFLSYLLSGAYGQALDGGVSSFEGKEGQAIFPEAFSVSSRPDHPQ
                     LIPFPYNGDGHIARPFTFIDKGIFRDFVMNHYYAHKLNRPKNGSVGVSALVMEPGDAS
                     LEQMIAGIDRGLYIANLHYMNFINRKETSATGLTRDGTFLIEKGKVVKVVNNLRYTVK
                     ISSVFE"
     gene            complement(1283..1972)
                     /gene="aqpZ"
                     /locus_tag="PKH10_11030"
     CDS             complement(1283..1972)
                     /gene="aqpZ"
                     /locus_tag="PKH10_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNT28694.1"
                     /translation="MKQYGAEFFGTFWLVLGGCGSAVLAAAFPGFGIGFVGVALAFGL
                     TVLTMAYAIGHISGCHLNPAVSIGLWAGGRFPANKLLPYIVAQVLGGLVAGGVLYLIA
                     TGKAGFDLAGGFASNGYGEHSPGGYSLLAALITEIVMTMMFLLIIMGATDKRAPQGFA
                     PIAIGLGLTLIHLISIPVTNTSVNPARSIGVALYAGGWALEQLWLFWVAPIIGAVLGA
                     IVYRFIGDDKA"
     gene            2145..>2658
                     /locus_tag="PKH10_11035"
     CDS             2145..>2658
                     /locus_tag="PKH10_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28695.1"
                     /translation="MSVSRLTAFFAIILLVACAATAPQATAPQKGTVTPQATPLATGE
                     LLEKDDNEPAWRATLKIHEYDPQSGIHYFYGAATAPDKEESRTKAVANAIAYVSKQMS
                     VRVKYGTNLYQEQLLGADAGYLKDVTIERGAEVTLKNYEPEIYSERWKRYGASTYDSW
                     ARLAVPATEWK"
BASE COUNT          591 a          805 c          799 g          463 t
ORIGIN      
        1 ctcgaagacc gacgatatct tcacggtgta gcggaggttg ttgaccacct tcaccacctt
       61 ccccttctct atcaggaagg tgccgtcgcg cgtgagcccc gtcgccgagg tctctttgcg
      121 gttgatgaag ttcatgtagt ggaggttggc gatgtagagc ccacggtcga tccccgcgat
      181 catctgttca agcgacgcgt cacccggctc catcaccagc gccgatacgc cgaccgatcc
      241 gttcttgggc cggttcagtt tatgcgcgta gtagtggttc atcacgaaat cgcggaatat
      301 ccctttgtcg atgaaggtga acggccgcgc gatgtgaccg tcgccgttgt acgggaacgg
      361 gatgagttgc gggtgatcgg ggcgcgacga gacgctgaac gcctccggga atatcgcctg
      421 tccctccttc ccttcgaagg aactgacacc gccgtcgagc gcctgtccat aggcccccga
      481 aaggaggtac gacaggaagg tgtggaccgc ctgcggcccg aggatgacgg tgtattctcc
      541 cggctcgacg tcggtgacgg ggagcgtcgc ccatttcatc ttgcgggcga gatcggccat
      601 gaacgccgcc ggatcgaagg ggtccagcga ggtgccgccg aaggagttga tgaccgtcac
      661 gccgttcgac cgtgccaccg ccttcatgtc gagcatgagc ggcgagacga agatgcgctt
      721 gtcgagaccg ttggactgga gcaagaaacc gtcggaggag acggtgatga aggtgccgta
      781 gatgtcgaag ccgagcgggc cggccgccgc ggtcgcctgt tcgaggatgt cgagtttgcg
      841 cgaaagcggc gccgaatcgg ccgccttcag gaagtcgcga tattcgtact gcgtcgggtc
      901 atcctcgaaa ctcgtgaaat cgggatcggg cggcaggtcg ttcaagagtc cgaccgcccg
      961 ttcgaccatc gcagaggcct tcgcgccgtc ggggtcggct atctggaacg agaacttccg
     1021 cttctcgcgc tcgaccgtgc cgttcaatac caccgtccgt ttggaaatgt tatagttcgc
     1081 ctgactgcgg tagaaccgca ggaaatcggt ctcccagtcg aggtaacgga accggaaccc
     1141 gtaccccggg aaccgcccca tgatcttctt gagttcgccc ttcagcctat ccatataacc
     1201 ctccgcgaag aaattcagaa gaatataacc attcaacgga ctaaatgcaa aaaagaaaag
     1261 acgatccggt cagatctgga tattacgcct tgtcgtcgcc gatgaaacgg tagacgatcg
     1321 cgcccagaac ggcgccgatt atcggagcga cccagaaaag ccacagttgt tccagcgccc
     1381 agccgccggc atagagcgca accccgatac tgcgggccgg gttgaccgag gtgttggtga
     1441 ccgggatgct gatgaggtgg atcagggtaa gcccgaggcc aatggctatc ggagcaaagc
     1501 cttgcggcgc ccgcttgtcg gtggcgccca tgatgatgag caggaacatc atggtcatga
     1561 cgatctcggt gatgagcgcg gcaagtagcg aataaccgcc gggcgaatgt tcgccatagc
     1621 cattcgacgc gaaaccgccc gccagatcga agccggcctt gccggtggcg atgaggtaaa
     1681 ggacgccgcc ggcgaccagg ccgccgagga cctgcgcgac gatgtagggc aacaatttat
     1741 tcgcgggaaa acggccaccc gcccacagcc cgatggagac cgccggatta agatggcaac
     1801 ccgagatgtg accgatcgcg taggccatgg tcaggaccgt caggccgaac gcaagggcga
     1861 caccgacaaa gccgatgccg aaacccggga acgcggccgc caacacggcg ctgccgcaac
     1921 cgccgagaac gagccagaat gtcccgaaaa actccgctcc gtactgcttc atgtctctgc
     1981 acctctcaat taatgtaata aaggcgggca acaggccaag tgtatgccct tccggaacgg
     2041 tggtcaagtt tcctgaccgt aacaacgggg aagcaggcaa aaaaattgac ttcacgcgac
     2101 gcgacggcga acatgtcgtg ataatcgctt caccggaggt cgttatgtcc gtatcgcgtc
     2161 ttaccgcctt ttttgccatc atcctcctcg tcgcttgtgc cgccaccgcg ccacaggcca
     2221 ccgcccccca gaaaggcacc gtcaccccgc aggccacacc gctcgcgacc ggtgaactgc
     2281 tcgaaaaaga tgataacgag ccggcatggc gcgccaccct gaagatacac gagtatgacc
     2341 ctcagagcgg cattcactac ttttacggcg cggcaacggc acccgacaag gaggaatcgc
     2401 gcaccaaagc ggtcgcgaac gccatcgcct atgtcagcaa acaaatgtcg gtgcgtgtaa
     2461 aatacggcac caacctctat caggaacaac tgctcggcgc cgatgcgggc tacctcaagg
     2521 atgtcaccat cgagcggggc gccgaggtga cgctgaagaa ctacgaaccc gagatatatt
     2581 ccgagcgctg gaaacggtac ggcgccagca cctacgactc gtgggcccgg ctcgccgtcc
     2641 cggcgaccga atggaaac
//
LOCUS       DAOQUE010000403         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_46415_length_2645_cov_4.160232, whole genome shotgun sequence.
ACCESSION   DAOQUE010000403 DAOQUE010000000
VERSION     DAOQUE010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46415_length_2645_cov_4.160232"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PKH10_11040"
     CDS             <1..1155
                     /locus_tag="PKH10_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28696.1"
                     /translation="TDLSGLRRLRAVGGSVTVEHNAQLPDLGKLIALISIGNELRIKG
                     NSALTRIGLPSLESTFTVWGEHNDQLASLDLPRLSTVSGALYLLNNRALTSLDGVPLL
                     SAVGQLILQENDVLSHIADTTLPLTYLSHLEVRDNPRFSSLRGLGSLVTAGAVILDDA
                     PELSSLEGLDMLSQATSISIERCGIVSLNPLKRLYQLNGDLVLRDNPILIHLSGPVPL
                     SFIGGTLVIHRNPALIDLVGLPALRSAGAVSITGNSALNSIAGMASLAMVYGPIEILG
                     NPALKKATSLLSLRTVNGGIALMMNDAVTEVAFPSLETVGGDIAVSYNPFLADMTFPV
                     LTALGGDLHVEENPTLPSCQAEELSDRLAGGYGWEGLAVIRGNDPAGVCR"
     gene            1237..>2645
                     /locus_tag="PKH10_11045"
     CDS             1237..>2645
                     /locus_tag="PKH10_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28697.1"
                     /translation="MKRIVSLLVVALAFFAVACDALSTRPGSFVVVFQWETGHEPDTT
                     AKDYYLWAYYQEWKGGDGKTFPADIETNAKSLNEAGPVKLASGATLDFSELTYGDNRF
                     VRAAIWDNEQRTGDPLYIGISELFSFKATDHDKVVAVNMKLQANAGVDETGEGGSFAI
                     EVRHNGVVATRVAESTVTLRFTVKNADTVVIANDLTFEKGLATLKLADLTQIDETTYE
                     YGPWDITTGWEGLGDTTYAVFGKSRNTLGYESAPQRADVYLDTTAPTPSVTPFPEVAK
                     LGDTIEVRFSFDEEVPAENLELNWRWLEFTLVENKADKSYTFSHTVTADDPESVADFH
                     LRATDTVGNATDHLPIGSLEIDRTPPWVEGALIDTTDDKEALKSGDVLTVSFTASEEL
                     SADPLLKIDDRTFTLDTKSENDLTYTYTYTVVDNDISGIKTITASLRDPAGNDATVGL
                     GFATFDLTAPTQSHGRVVP"
BASE COUNT          540 a          872 c          742 g          491 t
ORIGIN      
        1 accgaccttt ccgggctccg tcgcctgcgt gccgtcggcg gctcggtcac cgtcgaacac
       61 aatgcgcaac tccccgacct cggcaaactc attgcgctca tctccatcgg caacgaactg
      121 cgcatcaaag ggaacagcgc tctgacgcgc atcggtcttc cctcgctcga aagcaccttc
      181 acggtctggg gggagcacaa cgatcaactc gcctccctcg acctcccccg tctctccacc
      241 gtgtccggcg ccctgtacct tctcaacaac cgggccctga cctctctcga cggggtaccg
      301 ctcctctcgg cggtcggtca actcatcctg caggagaacg atgtgctctc gcatatcgcc
      361 gacacgacgc tccctctcac ctacctctca catctcgagg ttcgcgataa cccccgtttc
      421 tcctcgcttc gcgggctggg gtcgctcgtc accgccggtg cggtgatcct cgacgacgcg
      481 cccgagctct catcccttga gggtctcgat atgctctcac aggcgacctc tatttcgata
      541 gagcggtgcg ggatcgtgtc tctgaacccg cttaaacgcc tctatcaact gaacggcgat
      601 ctcgtactcc gcgacaaccc gatcctcatc cacctgtcgg gacccgtacc tctctctttc
      661 atcggcggca cactcgtgat acaccggaac ccggcgctca tcgatcttgt agggctcccg
      721 gcacttcgat cggcgggggc ggtatccatc acaggcaata gcgccctgaa ttccatcgcc
      781 ggcatggcat cgctcgcgat ggtctacgga cccatcgaga tactgggcaa ccccgcgctc
      841 aaaaaggcga catcgttgct atcgcttcga acggtgaacg gcgggatcgc cctcatgatg
      901 aacgacgcgg tcacggaggt ggccttcccc tctctcgaaa cggtcggcgg ggatatcgcc
      961 gtttcctaca atcccttcct cgccgacatg accttcccgg tactcaccgc gctcggcggc
     1021 gatcttcatg tcgaagagaa tccgacgctc ccctcgtgtc aggcggagga attgagcgac
     1081 cggctggcag ggggatacgg ctgggaaggg cttgccgtga tacgggggaa cgaccctgcc
     1141 ggggtttgtc gctaaagacc ctttcttttc cttgaaaaag tcacgcgggc atgctaccat
     1201 accccgaatt ttcggtctgt atcgggagcg ggaatcatga aaaggatagt aagcctcctc
     1261 gtcgtcgcct tggccttttt cgccgtcgcc tgcgacgcgc tgtcgacccg gcccgggtcg
     1321 ttcgtcgtgg tcttccagtg ggagacggga cacgagccgg acactacggc taaagattac
     1381 tatctctggg cctactatca ggaatggaaa gggggcgacg gcaagacctt ccccgccgac
     1441 atagagacga acgcgaaatc gctcaacgag gcgggaccgg tgaaactggc gagcggcgcg
     1501 acgctcgact tctcggaact gacctacggc gataaccggt tcgtgcgggc ggcgatatgg
     1561 gacaacgaac agcggaccgg cgacccgctc tacatcggca tatcggaact gttctccttc
     1621 aaggcgaccg atcatgacaa ggtggtcgcg gtcaacatga aactgcaggc gaacgccggc
     1681 gtggacgaga ccggcgaagg gggtagtttc gcgatcgaag tacggcacaa cggcgtcgtc
     1741 gcaacgcgag tggccgagtc gaccgtgacc ctgcgattca cggtgaagaa cgccgacacc
     1801 gtcgtcatcg ccaatgacct caccttcgaa aagggcctgg cgactctgaa actggctgac
     1861 ctcacgcaga tagacgaaac gacctacgaa tacggcccgt gggacatcac caccggctgg
     1921 gaagggctcg gcgacaccac ctatgcggtc ttcggcaaat cacgcaacac gctggggtac
     1981 gagagcgccc ctcaacgggc cgatgtctac ctcgacacca ccgcaccgac acccagcgtt
     2041 acgccgttcc ccgaggtggc caaactggga gacaccatcg aggtgcgttt ttcgttcgac
     2101 gaagaggtac cggcggagaa tctggaactg aattggcgtt ggctggagtt cacgctcgtc
     2161 gagaacaagg ccgataagag ttataccttc tcccatacag tgacggcgga cgatcccgaa
     2221 agcgtggccg atttccacct ccgggcgacc gatacggtcg gcaacgcgac cgaccatctg
     2281 ccgatcggtt cgcttgagat agaccggaca ccgccgtggg tcgagggcgc ccttatcgat
     2341 acgaccgacg acaaagaggc gctgaagagc ggcgatgtgc tgaccgtctc attcaccgcg
     2401 agcgaggaac tttccgccga cccccttctg aaaatagacg atcgtacctt tacgcttgac
     2461 accaagagcg agaacgatct cacctacacc tatacctaca ccgtcgtcga caatgacata
     2521 tccggcatca agacgataac cgcatccctg cgtgacccgg cagggaacga cgcgaccgtg
     2581 ggactgggct tcgcgacctt cgatctcacc gcgccgaccc agtcccacgg tcgcgtcgtt
     2641 ccctg
//
LOCUS       DAOQUE010000404         2631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_46806_length_2631_cov_4.295419, whole genome shotgun sequence.
ACCESSION   DAOQUE010000404 DAOQUE010000000
VERSION     DAOQUE010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46806_length_2631_cov_4.295419"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PKH10_11050"
     CDS             complement(<1..558)
                     /locus_tag="PKH10_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007798595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNT28698.1"
                     /translation="MGKVIGIDLGTTNSCVAIMEGGDPKVIENSEGARTTPSVVSFTD
                     KGERVVGQSAKRQAVTNPKRTLYSIKRLIGRRYEDAEVEKMKKVAPFEIVKADNGDAW
                     VRVEGTEYSPPQISAMTLQKMKETAESYLGEAVTDAVITVPAYFNDSQRQATKDAGAI
                     AGLNVLRIINEPTAAALAYGIDKKGK"
     gene            complement(551..1288)
                     /locus_tag="PKH10_11055"
     CDS             complement(551..1288)
                     /locus_tag="PKH10_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNT28699.1"
                     /translation="MSKKRKQSAPPPDGNGPVIELDEKAAATGMSLQEIADALGKQMM
                     QGKEEEKKEKEEEKEPEKEPAALAQKEEVSKKIKELESEVVRLVAENRNQKGRLENEF
                     RTRLKFATEDFFRDFIIVKDDMEKALTFVPDTGDEKVAAFADGIRHLNANIEALLRRF
                     GVESFDAIGTPFDPHLHQAMRMVDVAGRPAQEVVAQHLKGYRYYERVLRPAMVDVASG
                     NAPAEEKPVEAPQDIPTPNNDTEESHG"
     gene            complement(1285..2331)
                     /gene="hrcA"
                     /locus_tag="PKH10_11060"
     CDS             complement(1285..2331)
                     /gene="hrcA"
                     /locus_tag="PKH10_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNT28700.1"
                     /translation="MQPVEKTENSRLSPREEDILRSIVNLYAERGEAIGSQLLSDRVG
                     DLSSATVRNIFARIEERGLIRKAHCSAGRIPTDAGLRYFVDHILKVRRIAEGDRRLID
                     ERTEPVRGDVPSLLAAIPDALSEVSRYTGFVLSKRTRPAAIRHIDLVPVAPRRVLVIL
                     VDAIGGIANRVIELPEDVPAAELERLRNFINAHLVDRTLLEIRGHFLGELRSLQERYE
                     QVLDRIIAASPESVRTHTSGETNAFDYIGSADLRHLKRIMDDFRHTRLYLDLFSRIAS
                     SRDVHIFIGAESDLFCIPECAVILSSYDLQGGDVVGVVGVIGPRRMNYGKMISIVDYT
                     RHLLTARGADEEER"
     gene            2494..>2631
                     /locus_tag="PKH10_11065"
     CDS             2494..>2631
                     /locus_tag="PKH10_11065"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNT28701.1"
                     /translation="MEQAEKTAYWFEIAEYDLETARAMLKTKRYLYVGFMCHQTIEKA
                     LK"
BASE COUNT          455 a          810 c          791 g          575 t
ORIGIN      
        1 cttgcctttc ttgtcgatgc cgtaggcgag cgccgcggcg gtcggctcgt tgatgatgcg
       61 caggacattg agtcccgcga tggcaccggc gtccttggtc gcctgccgtt gagaatcgtt
      121 gaagtaggcg ggtaccgtga tgaccgcgtc ggtgaccgct tcaccgaggt acgattcggc
      181 ggtctccttc atcttttgca gggtcatcgc cgatatctgc ggcggcgaat actcggtccc
      241 ctccacgcgg acccacgcgt cgccgttgtc ggccttcacg atctcgaacg gggcgacctt
      301 cttcatcttc tcgacctcgg cgtcctcgta gcgacggccg atgagccgtt tgatggagta
      361 gagcgtccgc ttggggttgg tgaccgcctg ccgcttcgcg ctctggccga cgacccgttc
      421 ccctttatcg gtgaaggaga cgaccgacgg ggtggtccgc gccccttcgc tgttctcgat
      481 gaccttcgga tcgccgcctt ccataatggc gacgcacgaa ttggtcgtgc ccagatcgat
      541 gccgatgacc ttacccatga gattcctccg tatcgttgtt cggtgtcggt atatcctgcg
      601 gtgcttcgac cggtttctct tcggcggggg cgttgcccga ggcgacatcg accatcgccg
      661 ggcggagcac ccgctcgtaa taacggtagc ccttgaggtg ctgggccacc acctcctgcg
      721 cgggtctccc ggcgacatcg accatccgca tcgcctgatg caggtgcggg tcgaacggcg
      781 tcccgatcgc gtcgaacgac tcgacgccga accggcggag cagcgcctcg atgttggcgt
      841 tcaggtggcg gatgccgtcg gcgaaggcgg cgaccttctc gtcgcccgta tcaggcacga
      901 aggtgagcgc cttttccatg tcgtccttga cgatgatgaa gtcgcggaaa aaatcctcgg
      961 tcgcgaattt gagccgggta cggaactcat tctcgagccg ccctttctgg ttgcggttct
     1021 cggcgacgag ccggacaacc tcggattcca actcctttat ctttttagat acttcttcct
     1081 tctgcgccag cgcggcgggt tctttttccg gctccttttc ctcctctttc tccttttttt
     1141 cctcctcttt accctgcatc atctgtttgc cgagcgcgtc ggctatctcc tgcagggaca
     1201 tccccgtcgc ggccgccttc tcgtccagtt cgatgaccgg cccgttcccg tcgggcggcg
     1261 gcgcactctg ttttctcttt ttgctcatcg ctcctcctcg tccgccccgc gggcggtcaa
     1321 caggtgacgg gtataatcga cgatcgatat catcttgccg taattcatcc gtcgcggacc
     1381 gatgaccccg accacgccga cgacatcacc gccttggaga tcgtaactgc tcaatataac
     1441 ggcgcactcc ggtatgcaga agaggtcgga ttcggcgccg atgaagatgt ggacatcgcg
     1501 cgaactcgct atgcggctga agagatcgag gtacaggcgg gtatgccgga aatcatccat
     1561 gatcctcttg aggtggcgca ggtcggccga cccgatgtag tcgaacgcgt tggtctcacc
     1621 gctcgtatgg gtccgcaccg attcggggga cgcggcgatg atgcggtcga gcacctgctc
     1681 ataccgttcc tgcagcgacc gcagttcacc gaggaaatgg ccccgtatct caagaagcgt
     1741 gcggtcgacc agatgcgcgt tgatgaagtt gcgcaggcgc tccagttcgg ccgccgggac
     1801 atcctcgggc aactctatca cccggttggc gatgccgccg atggcgtcca cgagtatgac
     1861 gagcacccgg cgcggcgcga ccgggacgag atcgatgtgg cggatggcgg cgggacgggt
     1921 ccgcttcgag agcacgaacc cggtgtagcg cgacacctcc gacagtgcgt cggggatggc
     1981 ggccagcagc gacggcacat cgccccgcac cggctcggtc cgctcgtcta tgagccgccg
     2041 gtcgccctcc gcgatgcgac gcaccttgag gatgtgatcg acgaaatacc ggagccccgc
     2101 gtcggtcggg atgcggcccg ccgaacagtg cgccttgcgg atgagtcccc gttcctcaat
     2161 gcgggcaaat atgttgcgga cggtggccga cgacaggtcg ccgacccggt cgctcagcag
     2221 ttgcgacccg atcgcctcgc cccgttcggc gtagagattg acgatggagc gcaggatatc
     2281 ctcctcgcgg ggactgagtc ggctgttttc cgtcttttca acaggttgca ttcttatcac
     2341 cgtgaaaatg ccctacctat ataaagacgg gggggagaaa gtcaagcgaa ccgctctttt
     2401 atttaatgta tattagacac gcgaggggaa atacttatcc attgacaaaa cttcccgttt
     2461 cctatagtat gaacccgttt accggaggtg tcggtggaac aggcagagaa aacagcctat
     2521 tggttcgaga tcgccgaata cgatctagaa acggcacggg ccatgttgaa gacaaagcgt
     2581 tatttatatg tcggcttcat gtgccatcag accatcgaga aggcgctcaa g
//
LOCUS       DAOQUE010000405         2620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_47122_length_2620_cov_4.346199, whole genome shotgun sequence.
ACCESSION   DAOQUE010000405 DAOQUE010000000
VERSION     DAOQUE010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47122_length_2620_cov_4.346199"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /gene="hgcA"
                     /locus_tag="PKH10_11070"
     CDS             complement(<1..670)
                     /gene="hgcA"
                     /locus_tag="PKH10_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HNT28702.1"
                     /translation="MVLIQKQKTRAFSIATITLPGSPKDLIPLLSFRDRVEHLLVRIG
                     WRRRRYIVEPALYRIGSPMPDSPVIVTANYKLTVDTVRAACAGMDLWVLVLDTRGINV
                     WCAAGKGTFGTDEIERMVRTSRLTDIVKHRRLILPQLGAPGVSAHRVKKITGFTVIYG
                     PVRIADLRDFLAADMNATSDMRHVRFTLSDRISVTPIELVLSWQLFLAAALAGLGLWA
                     IHAAP"
     gene            complement(711..1751)
                     /gene="arsB"
                     /locus_tag="PKH10_11075"
     CDS             complement(711..1751)
                     /gene="arsB"
                     /locus_tag="PKH10_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNT28703.1"
                     /translation="MKSLSFLDRYLTLWIFLAMGVGVLAGWAYPGVADLINYFSYGTT
                     SIPIAVGLILMMYPPLAKVRYERMGDILKNRKVLVLSLIQNWIVGPVLMFGLAVLFLS
                     DKPEYMIGLIMIGLARCIAMVIVWNDLAKGDREYCAALVAFNSIFQVLLFSVYAWFFI
                     TFLPQKLGLAGAVVNITIGQIAVTVFIFLGIPFLAGLFSRYGLIAAKGRAWFDSVYAP
                     KIGPITLIALLFTILVMFSLKGEYIVNLPFDVIRIAIPLAIYFAVMFVVSFWMSKAVG
                     ATYEESATLSFTAASNNFELAIAVAVATFGINHGAAFSAVIGPLIEVPALILLVNLAF
                     YFRRRWYGFSGS"
     gene            complement(1764..2207)
                     /locus_tag="PKH10_11080"
     CDS             complement(1764..2207)
                     /locus_tag="PKH10_11080"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNT28704.1"
                     /translation="MIRVLFVCIHNSARSQMAKAYLNALGGGRFEAKSAGIEPGKLNP
                     VVVEAMRLDGIDISQNRVKAVKEFIDRGEKFDYLITVCDETAADRCPFVPGTGQRLHF
                     GFSDPSAFGGTPEEKLAATIAVRDAIKKKITEFILTVRASQRNAP"
     gene            complement(2217..>2620)
                     /locus_tag="PKH10_11085"
     CDS             complement(2217..>2620)
                     /locus_tag="PKH10_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNT28705.1"
                     /translation="ASWGFAKQILPLLLMGVFAAGLFLGRPGNEGIIPNEWVAAVVGG
                     NSFFANFFASIVGAFMYFATLTEVPIVQGLMGSGMGKGPALSLLLAGPALSLPNMLVI
                     RSVIGTKMTVVFVTLVIVMATVTGMIYGALF"
BASE COUNT          573 a          753 c          826 g          468 t
ORIGIN      
        1 acggtgcggc atgaatggcc cagagcccta gccctgccaa ggccgccgcg aggaagagtt
       61 gccacgacag caccagttct atcggggtca cggagatccg gtcggaaagg gtgaaccgga
      121 cgtgccgcat gtcgctggtc gcgttcatgt cggcggcgag gaagtcccgc aggtccgcta
      181 tccgcactgg tccgtagatg accgtgaatc cggtgatttt tttcacacgg tgtgccgaaa
      241 cgccgggggc acccaattgc ggcaggatga gtcggcgatg cttcacgatg tcggtaagac
      301 ggcttgttct caccatccgt tcgatctcat cggtaccgaa ggttcctttt cccgcagcgc
      361 accagacatt gatgccgcgc gtgtcgagga cgagcaccca gaggtccatc ccggcgcagg
      421 cggcgcggac cgtgtcgacg gtcagtttgt agttggcggt cacgatgacg gggctatcgg
      481 gcatcggtga tccgatgcgg tagagcgccg gttcgacgat atatctgcgg cggcgccaac
      541 cgatacggac aaggagatgt tccacccggt cacgaaacga aaggagaggt atgagatcct
      601 taggagaacc cggtagcgtg atggtggcta tggagaaggc gcgagttttc tgcttttgga
      661 tgagcaccat atcttctcgt tcctcaatcg gtcgggggtc agtggctctc ctacgatcct
      721 gaaaatccgt accaacgccg cctgaaatag aaggcgagat tcaccaagag aatgagcgcc
      781 ggcacctcga tgagcggccc gatgaccgcc gagaaagcgg cgccgtggtt gataccgaag
      841 gtggcgaccg cgaccgcgat ggccagctcg aagttgttcg acgcggcggt gaacgacagc
      901 gtcgccgact cctcgtaggt cgcgccgacc gccttggaca tccagaacga aacgacgaac
      961 atcactgcga aatagatggc gagcggtatc gcgatgcgga tgacatcgaa cggcagattg
     1021 acgatgtatt cgcccttgag cgagaacatg acaaggatgg tgaagaggag cgcaatgagc
     1081 gttatggggc cgatcttcgg cgcataaacg gagtcgaacc acgcgcgtcc cttcgccgcg
     1141 atgagtccgt agcggctgaa aagccccgcg aggaacggga tgccgaggaa gatgaagacg
     1201 gtgaccgcta tctgcccgat ggtgatattg acgaccgccc cggcgagacc gagtttctgc
     1261 gggaggaagg tgatgaagaa ccacgcgtag accgagaaga ggagcacctg gaagatcgaa
     1321 ttgaacgcga ccagcgccgc gcagtattct cggtcgccct tcgcgaggtc gttccagacg
     1381 atgaccatcg cgatgcagcg ggcaagcccg atcatgatga gtccgatcat gtattccggc
     1441 ttatcggaaa ggaatagcac cgcgagcccg aacataagca ccggcccgac gatccagttc
     1501 tggataagcg acagcacgag aactttcctg ttcttcagga tgtcgcccat ccgttcgtag
     1561 cgcaccttgg ccagcggcgg atacatcatc aggatgagcc ccaccgcgat cgggatggag
     1621 gtggtgccgt aactgaaata gttgatgaga tcggcgacgc cgggataggc ccagccggcg
     1681 aggacgccga cccccatcgc gaggaagatc cacagcgtca ggtagcggtc gaggaaagaa
     1741 agacttttca tgccggcctc cgtttagggg gcgttccgtt gggacgccct taccgttagg
     1801 atgaattcgg ttattttctt ttttatcgcg tcgcgcacgg cgatggtcgc cgccagtttc
     1861 tcctcgggcg tcccgccgaa cgccgagggg tcggaaaacc cgaaatggag ccgctgtccc
     1921 gtgccgggga cgaagggaca acgatcggcc gccgtctcat cgcagacggt gataagatag
     1981 tcgaactttt caccgcgatc gatgaactcc ttgaccgcct tcacccggtt ctgtgagatg
     2041 tcgataccgt ccaagcgcat cgcctcgacg acgaccgggt tcaacttccc cggctcgata
     2101 ccggcgcttt tcgcctcgaa tcggccaccc cccagcgcgt tgagataggc cttagccatc
     2161 tgactgcggg ccgaattatg gatgcagaca aagagtacgc ggatcatggt gacctcctaa
     2221 aagagtgcgc cgtagatcat gccggtcacg gtcgccatca cgatgacgag cgtgacgaac
     2281 acgacggtca tcttcgtgcc aatgacgctg cggatgacca gcatgttggg cagagagagc
     2341 gccggaccgg cgagaagcaa agagagcgcc ggccctttcc ccatgccgct ccccatgagt
     2401 ccctgcacga tggggacctc ggtcagtgtc gcgaaataca tgaaggcgcc aacgatcgag
     2461 gcgaagaagt tggcgaagaa cgagttgccg ccgaccaccg ccgctaccca ttcgttcggg
     2521 atgatccctt cgttccccgg tcggccaagg aagagccccg ccgcgaagac ccccatgagg
     2581 aggagcggca ggatctgttt ggcgaagccc cacgatgccg
//
LOCUS       DAOQUE010000406         2617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_47224_length_2617_cov_4.092896, whole genome shotgun sequence.
ACCESSION   DAOQUE010000406 DAOQUE010000000
VERSION     DAOQUE010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47224_length_2617_cov_4.092896"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="PKH10_11090"
     CDS             <1..1309
                     /locus_tag="PKH10_11090"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNT28706.1"
                     /translation="PRVEPALCGMAPYHWLPSTGMGDIIEFDQMFGSLGDIAQTSKVA
                     LTLIKTQLENDGEVNLQRDPAYDTLVFRILYKTQDKGQQVEATGTIAVPLSGKTFPIL
                     MVQHGTAGLGDPCSPSAGDELNGTAMVAALFASFGYIAIAPDYIGLKSMGTPSTELHS
                     YLIGEPTAIASLDMVRAVPKFLARPEIQLLGIDPKPGKVIISGGSQGGHAAAFTTRFA
                     PYYAPELDIKGAVWGIPPTDIYSHAVRALNNYVPASGNTALVFLSANDWYRDEPMMLS
                     DVFIEPWDSTLRTHGLTSCDLDGPLDDAGVDTLEELFTPELLAAAKLDPFCDGFEPWL
                     CYIKENTLSRSSVPRIDTIPAFMILSENDTLVVPFIERDAFAELCDQGYQIQYRECTG
                     ASHSDGFLWSLDDQLDWLDDRYNDVPMTDVCTIHPAVKCKSAP"
     gene            complement(1322..>2617)
                     /locus_tag="PKH10_11095"
     CDS             complement(1322..>2617)
                     /locus_tag="PKH10_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT28707.1"
                     /translation="VIDEPGRGLHPADRATLGELLRELPAAGNTLITVDHAPQLMALA
                     DRMVELGPGAGAEGGCITAAGTPADLLRPGVTLSGDHLHGTVPERKSGAPESVIHIEK
                     AHAHNLKDITLDIPCQCLPAKACGGGVVVALTGVSGSGKSTLLFEIVAASAAAGSPRG
                     CAAIEGLERFSEVLSLERLRHTASVMSTLLTATGLADRLRTLFSATAEAKERGLTKSH
                     FSFLTAAGRCETCKGTGRETVALDFLPDVEAPCPACNGTRFNPEVLTIHLHGKNIADI
                     HALTVRDGAALFNGDKMMAHGLAILEKAGLGYLTMGQATDRCSAGELQRLHLAARLIA
                     GKGKEQVLYLLDEPEAGLHPADVAELASLFADLARQGSTILMATHSEPLIAASDHLVE
                     LGPGGGPDGGRVLFSGMPWELLCREDSPTGTALGLWRGG"
BASE COUNT          466 a          886 c          793 g          472 t
ORIGIN      
        1 cccgcgcgtc gaaccggcgc tttgcgggat ggctccctac cactggttgc catcgaccgg
       61 catgggcgac atcatcgagt tcgaccagat gttcggatcg ctcggcgaca tcgctcagac
      121 cagcaaggtg gcgttgacgc ttatcaagac acaactcgag aatgatggcg aggtcaacct
      181 ccagcgcgac ccggcgtacg acacgctcgt gttccgcatc ctctataaga cgcaggacaa
      241 gggccaacag gtcgaggcga ccgggaccat cgcggtgccg ctctccggaa agaccttccc
      301 catcctcatg gtacagcacg gcaccgccgg actcggtgac ccgtgctccc cttcagcagg
      361 tgacgaactg aacgggacgg cgatggtcgc cgcgctgttc gcctcgttcg gctacatcgc
      421 catcgcccct gattatatcg gtctcaagag catggggacc ccttcgaccg aactccactc
      481 ctatctcatc ggcgaaccga cggccatcgc gtcgctcgac atggtgcgcg ccgtaccgaa
      541 gttcctcgcg cgtcccgaga tacaacttct cggcatcgac ccgaaaccgg gcaaggtcat
      601 catatcgggc ggttcgcagg ggggccacgc cgccgccttc accacccgct tcgcccccta
      661 ctacgccccc gaactcgaca tcaaaggggc ggtctggggc atcccaccga ccgacatcta
      721 ttcccacgcc gtccgcgccc tgaacaacta tgttccggcc tcaggcaaca ccgcactcgt
      781 cttcttgtcg gccaacgact ggtaccgaga cgagccgatg atgctctccg atgttttcat
      841 cgaaccgtgg gacagcaccc tgcgtaccca cgggctgacc agttgcgatc ttgacggacc
      901 gctcgacgac gcaggcgtcg atacgcttga ggaactcttc acccccgagc tgcttgccgc
      961 ggcgaaactc gacccgttct gtgacggttt cgaaccgtgg ctctgttaca tcaaggagaa
     1021 tacgctttcg cgcagttcgg tgcctcgcat cgacacgata ccggcgttca tgatactttc
     1081 ggagaacgat acgctggtgg tgccgttcat cgaacgcgac gccttcgctg aactctgcga
     1141 tcagggctac cagatacagt accgcgagtg caccggcgct tcacacagcg acgggttcct
     1201 ctggtcgctc gacgatcagt tggattggct tgatgaccga tacaacgatg tcccgatgac
     1261 cgatgtctgc accatccacc cggcggtcaa gtgcaagagc gcgccgtaga atcgttcatc
     1321 atcagccccc ccgccataac cccaacgccg ttcccgtcgg agaatcctcc ctgcataaca
     1381 gttcccatgg catgccgctg aaaagcaccc gtccgccatc gggacctccg ccggggccga
     1441 gttcgaccag atggtcggac gccgcgatga gcggttcgct gtgggtcgcc atgaggatgg
     1501 tcgacccctg ccgcgcaaga tcagcgaaca gggacgccag ttcggcgaca tcggccggat
     1561 gaagccccgc ctccggttcg tcgagaagat agagcacctg ttcctttccc ttcccggcga
     1621 tgagccgcgc cgcgaggtgg agccgctgga gttcgcccgc cgaacagcgg tcggtcgcct
     1681 gtcccatggt gagatagccg agccccgcct tttcgagaat ggccagtccg tgcgccatca
     1741 tcttatcgcc gttgaacagc gccgcaccgt cgcgtaccgt cagggcgtgg atatcggcga
     1801 tgttcttccc gtgcaggtgg atggtcaaga cctcggggtt gaagcgcgtg ccgttgcagg
     1861 cggggcaggg cgcctcgaca tcggggagga agtcgagcgc caccgtttcg cggcccgttc
     1921 ccttgcaggt ctcgcaccgg cccgccgcgg tgaggaaact gaaatggctc ttggtgagcc
     1981 cccgctcttt cgcttcggcg gtcgcggaga aaagagtgcg cagacggtcg gcgagtcccg
     2041 tggcggtcag gagcgtgctc atgaccgatg cggtgtggcg caaccgttcg agcgacagca
     2101 cctcgctgaa ccgttcgagt ccctcgatcg cggcgcaccc gcgcggcgac ccggccgcgg
     2161 cgctcgccgc gacgatctcg aacagaagcg tcgatttacc gcttcccgaa acgccggtga
     2221 gcgccacgac cacgccgccg ccacaggctt tggcgggcaa gcactggcac gggatatcga
     2281 gcgtgatatc tttcaggttg tgggcgtgtg ctttttcgat atggatgacc gactccggcg
     2341 caccgctctt tctctccggg accgtaccgt gcagatggtc gcccgaaagc gtcacgccgg
     2401 gccgcaacag atcggcgggt gttccggcgg cggtgataca gccaccctcc gcgccggcgc
     2461 cggggccgag ttcgaccatc cgatcagcga gcgccatcag ttgcggcgcg tggtcgacgg
     2521 tgatgagcgt attgcccgcg gccggcagtt cacgcaggag ttcgccgagc gtcgcgcggt
     2581 cggcgggatg gagtccccgc cccggctcgt ctatcac
//
LOCUS       DAOQUE010000407         2604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_47607_length_2604_cov_4.184778, whole genome shotgun sequence.
ACCESSION   DAOQUE010000407 DAOQUE010000000
VERSION     DAOQUE010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47607_length_2604_cov_4.184778"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PKH10_11100"
     CDS             complement(<1..408)
                     /locus_tag="PKH10_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNT28708.1"
                     /translation="MHPDDTSRLRRYLDFVKIEKRLSRNSVAAYENDLSQFFAHLDTA
                     GKDLAATDHLTLTDYLIEISKDLRLKDRSLARKISAIRGFLRFLLEREGAPVTPLIEA
                     MEPVKLARTLPVFLSPDEMQGLLNAIDTSTEQGF"
     gene            complement(411..1232)
                     /gene="asd"
                     /locus_tag="PKH10_11105"
     CDS             complement(411..1232)
                     /gene="asd"
                     /locus_tag="PKH10_11105"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNT28709.1"
                     /translation="MSSLLIRTCLFFLSFPGLSRFYGRLVRIERPRWLVRWVIRLFAR
                     RYRISMNDFTGEFGDYASLSAFFTRPLDPAKRPMPADDRHILAPADSVLSVCETVRDD
                     RATQVKGRQYRLTGLVGEPFDLSRGWRVAVFYLSPHDYHRFHLPMAATVIAARRDGGS
                     LYPVNLLSVSNIPDLFIRNERVTLKCETAGEVWYYVAVGATFVGAVSTVAGVIPTAGS
                     WYPVGRELPQSAETGRFDMGSTIVTLIPVALAGEPLVAEGEKVRTGDRIWERRGG"
     gene            complement(1235..1732)
                     /locus_tag="PKH10_11110"
     CDS             complement(1235..1732)
                     /locus_tag="PKH10_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28710.1"
                     /translation="MGRFLIVPALLAAFLLSPFYARAEDTTEQNRDDIVLKGAERVHP
                     YLWHGAGVAGMGGLLLILGVTFNALADREYDTYRDMLTDERITAAINAGEKKETYLTR
                     VNDHLNEGKEYAATRTALYISGGILALTGSVLMLLTEEKRDDTAKFTASFDSNGVYAA
                     LSLSF"
     gene            complement(1800..2441)
                     /locus_tag="PKH10_11115"
     CDS             complement(1800..2441)
                     /locus_tag="PKH10_11115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNT28711.1"
                     /translation="MELKREHFREGLLRFALELTLIYLLPVIALKLLFPGREFSRHFK
                     EIMAFFVCWSAAAGYYAHRRGFSWKDMGFTAQGLPRSLLYNLLLMAIAAGVIVLIAGP
                     RLFDAPRMPRATHWFPLFYIFLSCPAQEFLYRGLLFGLMERAGLRHPALLILLTAATY
                     ASMHILFHKPLIVPLTFAIGLVWGALYWRVRNLWGLIAAHIALGFLSMAAGLA"
BASE COUNT          529 a          797 c          807 g          471 t
ORIGIN      
        1 gaatccctgt tcggtcgagg tgtcgatggc gttcaggagc ccctgcatct cgtcgggcga
       61 gaggaacacc ggcagggtgc gcgccagttt gaccggctcc atcgcctcga taagcggggt
      121 gaccggcgcc ccctcccgtt cgagcaggaa gcggaggaac ccgcggatcg ccgatatctt
      181 gcgggcgagc gaccggtcct tgagtctcag atccttcgat atctcgatca gatagtcggt
      241 gagcgtcagg tgatcggtcg cggcgaggtc cttccccgcg gtgtcgagat gggcgaagaa
      301 ctgggaaaga tcgttctcgt aagcggctac gctgttgcgg gacagtcgtt tttctatctt
      361 gacgaaatcg agatacctgc ggagtcgcga ggtgtcgtcg ggatgcatgg ctacccgccc
      421 ctccgttccc agatgcggtc acccgtccgg accttttccc cctcggcgac gagcggttct
      481 cccgccagcg ccaccgggat gagcgtcacg atggtcgacc ccatgtcgaa ccggcccgtt
      541 tcggccgact ggggcagttc ccgacccacc ggataccacg agcccgccgt cgggatgacg
      601 cccgcgaccg tcgagacggc cccgacgaag gtcgcgccca ccgcgacata gtaccagacc
      661 tcgcccgccg tctcgcattt gagcgtcacc cgctcgttgc ggatgaaaag gtcggggata
      721 ttgctcacag agagaagatt gaccggatag agcgatccgc catcgcgtcg cgccgcgatg
      781 accgtcgccg ccatcggcag atggaaccgg tggtaatcgt gcggcgagag gtaaaacacc
      841 gcgacccgcc agccccgcga gagatcaaag ggctccccca ccagtccggt cagacggtac
      901 tggcgtccct tcacctgcgt cgcccggtcg tcacgaaccg tttcacagac gctgagcacg
      961 ctgtcggcgg gagcgaggat gtgccggtcg tcggcgggca tcggccgttt tgccggatcg
     1021 agcgggcggg tgaagaaggc ggaaagactc gcatagtcgc cgaactcccc cgtgaaatcg
     1081 ttcattgata tgcggtaacg gcgcgcgaag agccgtatca cccagcggac caaccatcgg
     1141 ggccgctcga tgcggacgag gcgaccatag aaacggctca agccggggaa cgaaaggaag
     1201 aacaggcatg tccggataag cagggaggac atcgtcagaa cgagaggctc agggcggcgt
     1261 agacgccatt gctgtcgaat gacgcagtga atttcgcggt atcatccctt ttctcctcgg
     1321 taaggagcat gagcaccgag ccggtcagcg ccagtatacc gcccgatata tagagtgcgg
     1381 tgcgggtcgc ggcatattcc ttcccctcgt tgaggtgatc gttcacgcgg gtaaggtagg
     1441 tctccttctt ctcgcccgcg ttgatggcgg ccgtgatacg ctcatcggtc aacatgtcgc
     1501 ggtaggtgtc gtactctcgg tcggcgagcg cgttgaaggt gacgccgagt atcagcagga
     1561 gaccgcccat gccggcgacc ccggcgccat gccagagata cgggtggacc cgttcggccc
     1621 ctttcaacac gatatcgtcc ctattctgtt cggtggtgtc ctcggcccgg gcgtaaaatg
     1681 gcgacagcag gaatgcggcc agcaacgccg gaactatcag aaaacgaccc atctctctcc
     1741 tccatccgta caagccgggg cgattatagt cgcttttccc gtcgtggcaa atataaaaat
     1801 cacgcgagcc ccgccgccat cgaaaggaac cccagcgcga tgtgcgccgc gatgagtccc
     1861 caaagattcc gcacccgcca gtagagagcc ccccatacca accctatcgc gaaggtcagc
     1921 ggcacgatga gcggcttgtg gaaaaggata tgcatcgacg cgtaggtcgc cgccgtgagc
     1981 agtatcagga gcgccggatg acgaaggccg gcccgttcca tgagcccgaa caggagtccg
     2041 cggtacagga actcctgtgc cggacacgag agaaagatgt aaaagagcgg gaaccagtgg
     2101 gtggcgcggg gcatacgcgg ggcatcgaac agccgcggac cggcgatgag gactatgacc
     2161 cccgccgcga tcgccatgag cagcaggttg tacaggagcg aacggggcaa gccctgcgcg
     2221 gtgaagccca tatccttcca cgagaacccg cgccggtggg cataatatcc cgccgccgcc
     2281 gaccaacaga cgaaaaaggc catgatctcc ttgaaatggc gggagaactc gcgtcccggg
     2341 aacaacagtt tgagcgcgat gaccggtaac aggtagatga gcgtcagttc caacgcgaaa
     2401 cggaggaggc cctctctgaa atgttctctc ttcagttcca tgcgcacatc ttatttacat
     2461 ttttttctat tgcaacctgt cccgtttgcc ggtactatgg tgcggtcatt ctaccggagg
     2521 taccccatgc gttctttttt tccgttgctc ccgatacttt ttcttatggt catcgcctgc
     2581 gacggcgtga cctacggcgg ccgc
//
LOCUS       DAOQUE010000408         2590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_48013_length_2590_cov_4.927022, whole genome shotgun sequence.
ACCESSION   DAOQUE010000408 DAOQUE010000000
VERSION     DAOQUE010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48013_length_2590_cov_4.927022"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PKH10_11120"
     CDS             complement(<1..477)
                     /locus_tag="PKH10_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28712.1"
                     /translation="MGYIGLFLISVAVLALQILQTRIFSFTLWHHFAYLVITIALMGM
                     GAAGSWLAIRKKESDRPYLTLARHATLFGLFAVVSFMIVVRVPLDTYMSDRLLQMGYV
                     FLYYLALIFPYFFAGAVIALMFRADRTRINLLYLVNLTGSAIGGFAVIFIIERWGGA"
     gene            complement(518..1405)
                     /gene="truB"
                     /locus_tag="PKH10_11125"
     CDS             complement(518..1405)
                     /gene="truB"
                     /locus_tag="PKH10_11125"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNT28713.1"
                     /translation="MSPDNGSFFLSFYKDPGITSAGALDKLKRALKLPKAGHCGTLDP
                     MAEGLLVVAVNRATKLMRFVTDEPKRYVGRFEIGKKSPSYDSETTMEVTAPDADTSQV
                     DWQRILSIFNGRIRQTPPAYSAVRVDGVRSYRLARKGQLSDLPPKEVVIHSLALKTID
                     RTTVGFTVECSKGTYIRSLVHDIGLVAGTGAVLVQLTREAVGRFTVVEAVRLDDLRAA
                     PDKVDRAKLPLPAFLRRFPSITVNETLFWQLRNGIDIRSLGLPLVDGPNTVLYNDEPL
                     FLLEKEGKECRYTAYFGSD"
     gene            complement(1392..2366)
                     /locus_tag="PKH10_11130"
     CDS             complement(1392..2366)
                     /locus_tag="PKH10_11130"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNT28714.1"
                     /translation="MTTAHSELSGIIRRSRTALVSGHVNPDGDSVGSALACYHLLTGQ
                     GIATTLYSIDPFPYNFLFLPGADRMTHELPAEEPDLYLIVDAGAPHRVGEPLHRRLQS
                     TKSPKILFDHHVNYHDQGSFFDRTFIDEHAAATAILLYRFITDQGFPMTREIAVAIYA
                     AVMADTGSMRYDSTDREAFLALGDLVTWVDPWEIAAKIWEETPLSQLRLLGEVLSSIR
                     LLAGGKAAVFEITREQLRRYHLGPDHVDGFINYARGIEGVEIAMRFREIGPRLYRVSM
                     RSKGSIDAGRISHEFGGGGHRNAAGFDFNGSFDEACQLVEEIVARVAR"
     gene            complement(2347..>2590)
                     /locus_tag="PKH10_11135"
     CDS             complement(2347..>2590)
                     /locus_tag="PKH10_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28715.1"
                     /translation="RSAMVRYALMPGTPRRLTQDRLEEEMKHIRYLLAREIRHIKFMP
                     ELDFRFNDRAEEEARLQELLDGLSREREARGDDGAQ"
BASE COUNT          492 a          755 c          869 g          474 t
ORIGIN      
        1 ggcgccgccc cagcgttcga tgatgaagat gaccgcgaaa ccgccgatgg ccgagccggt
       61 aaggttgacg agatagagca ggttgatgcg ggtccggtcg gcgcggaaca tgagcgcgat
      121 caccgcgccg gcgaagaagt aggggaatat gagcgcgagg tagtacagga acacatagcc
      181 catctggagc aggcggtcgc tcatgtaggt gtcgagcggc acgcggacga cgatcatgaa
      241 cgacacgacg gcgaaaagcc cgaaaagcgt cgcgtgccgg gcgagcgtca gataggggcg
      301 gtcgctctct tttttacgaa tggcgagcca cgagccggcg gcgcccatgc ccatgagcgc
      361 gatggtgatg accagatagg cgaagtgatg ccagagggtg aaggagaata tccgtgtctg
      421 cagtatctgg agcgccagta ccgcgaccga tatgaggaat aagccgatgt agcccatgaa
      481 tgcctcccgg gatgcggttc actatctcgt cggtcggtca gtcggatccg aaataagccg
      541 tgtagcggca ttccttgccc tctttttcaa ggaggaacaa gggttcgtcg ttatagagga
      601 cggtgttcgg tccatccaca agcggcaggc cgagagaacg gatatcgatg ccgttgcgca
      661 gttgccaaaa gagcgtctcg ttgacggtga tcgaggggaa ccgccggaga aaggcgggga
      721 gcggcagttt tgcccggtcc accttgtccg gcgccgcgcg gagatcgtcg agccgcaccg
      781 cctcgacgac ggtgaagcgc ccgaccgcct cgcgggtgag ttgtacaagc accgcgccgg
      841 taccggcgac cagcccgatg tcgtgaacga gcgaccggat gtaggtgccc ttggagcatt
      901 cgacggtgaa tccgacggtc gtccggtcga tggtcttcaa ggcaagcgag tgtatgacga
      961 cctctttggg cggcaggtcg gataactggc ccttgcgtgc cagtcggtag gatcggacgc
     1021 catcgacccg caccgccgaa taggcgggag gcgtctggcg tatccggccg ttgaatatgg
     1081 aaagtatccg ttgccagtcc acctgcgaag tgtcggcgtc cggagcggtc acctccatcg
     1141 tggtctcgct gtcgtagctg gggcttttct tgcctatctc gaaccggccg acataccgtt
     1201 tcggctcgtc ggtgacgaac cgcatgagtt tggtagcgcg gttcaccgcg acgacaagta
     1261 gcccttcggc catcgggtcg agtgtgccgc agtgccccgc tttgggcaat ttgagggccc
     1321 gcttgagttt gtcgagagcg ccggccgagg tgatgccggg gtctttgtag aaagaaagga
     1381 agaaggatcc gttatcgggc gacacgggcg acgatctcct ccaccagttg acaggcctcg
     1441 tcgaacgatc cgttgaagtc gaaccccgcg gcattgcggt ggccgccgcc gccgaactcg
     1501 tgcgatatcc gccccgcgtc tatcgacccc ttgctacgca tcgagacgcg gtagagccgg
     1561 ggtccgatct cgcggaaccg catggcgatc tcgaccccct cgatgccgcg tgcgtagttg
     1621 atgaagccgt caacatggtc ggggccaaga tggtagcggc gcaactgttc ccgcgttatc
     1681 tcgaacaccg ccgccttgcc gccggcaagg aggcggatcg aagaaaggac ctctccgagc
     1741 aggcgcaatt gggaaagcgg cgtctcttcc catatcttcg ccgcgatctc ccaagggtcg
     1801 acccatgtca cgaggtcgcc gagcgcaagg aaggcctcgc ggtcggtcga gtcgtagcgc
     1861 atactgccgg tgtcggccat caccgccgcg tagatcgcga cggcgatctc acgggtcatc
     1921 gggaacccct gatcggtgat gaatcggtac aggagtatcg ccgtggccgc cgcgtgctcg
     1981 tcgatgaagg tccggtcgaa gaacgacccc tgatcgtggt agttcacatg gtggtcgaac
     2041 agtatcttgg gacttttcgt gctctgcaga cggcggtgga gcggttcgcc gacccgatgg
     2101 ggggcgccgg cgtccacgat gagatagagg tccggctcct ctgcgggcag ttcgtgcgtc
     2161 atccggtcgg cgccgggcag gaagaggaaa ttatagggga aagggtcgat cgaatagagg
     2221 gtggtcgcga tgccctgccc ggtcaggaga tggtagcagg cgagcgccga gccgacactg
     2281 tcgccgtcgg gattgacatg cccggacacc agcgcggtcc gcgaccgcct tatgataccg
     2341 gagagttcac tgtgcgccgt cgtcaccgcg cgcctcccgt tcccggctca ggccgtcgag
     2401 cagttcctgc aggcgcgcct cttcctccgc ccgatcgttg aaccggaaat ccagttccgg
     2461 catgaacttt atgtggcgta tctcgcgggc cagcagatag cgtatatgct tcatctcctc
     2521 ttcgagccgg tcctgcgtga gtcggcgcgg agtgcccggc atcaacgcgt aacggaccat
     2581 cgcgctacgc
//
LOCUS       DAOQUE010000409         2573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_48499_length_2573_cov_6.640191, whole genome shotgun sequence.
ACCESSION   DAOQUE010000409 DAOQUE010000000
VERSION     DAOQUE010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48499_length_2573_cov_6.640191"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2573)
                     /locus_tag="PKH10_11140"
     CDS             complement(<1..>2573)
                     /locus_tag="PKH10_11140"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNT28716.1"
                     /translation="TVQWTQQFGTADNEAETRLILDAEDYIYLVGTTTGAFSGTNAGG
                     TDLFVARYDMFGYQSWVKQRGSAGNEETRFITIDDAGNLYVAGATNGDLDGNTNAGGS
                     CSGSTCADIYIMKFSDEPSKVWPKQWGSTLNDYGYANAADPQGNIVVGGISNGNVDDQ
                     TVTSSGTTCGSNPCSDNLLTKWMPNGTRLWTRMWGSDKTDVVNGVAIDGTGNIYAAGY
                     TNGDLDGNTNAGSSDVFLTKYLPDGTKAWTKLWGNASANIGNAVTVDGSGNIYVTGKT
                     SGGLDGKSAIGGEDIFITKWHDTGSDATKEWTKVWGNTSGDVGYDLLIDSNNNLYITG
                     ASYGGTGTQKDGETYSGNYDLVLMKWSIGADDNSTVRVWTKLWGSSTADYAYSLEKNQ
                     DQDLYVTGYTTGTLPSGSLPSNTAQGGYDAFLSRWTQDGSTATLAWTSQWGTPQRTAV
                     QIINWPLTSSGSTYSSAGYGYGVFSGGTGIGTITYNASGATANNWSTGAMDSNDYYQV
                     YFYPNPGNAVTVTAINFSEKRSSNGIRNYKVQYSKSSSFTNPVDLATVAVPDNTETRS
                     GNITGLSISVGLGESIYIRFYGYNSEGQNGTWTIAPNVNMQATSVGMIDQATSLALDT
                     QGNIYVGGLTRGGIDGYTNVYGGYEDYFISKYVADGSRIWTYQNGSTYADRLWGLVRD
                     NSDNLYASGYTLGTLPNNTSAGNADIFLLKVSENGGSLETQTVSCVEDPDRPENSQSL
                     SSTVSVSYSSAGGWSQPAYCEWKCEDEYVRRGMFCEEKICDEGVRSTVACTVVESLIT
                     GVRYRICVDNAWEDETGECVIGCTPDTSMFIVDNTNCSNSGQKRYVCNENGALEPASD
                     E"
BASE COUNT          545 a          751 c          599 g          678 t
ORIGIN      
        1 ctcgtcgctt gccggttcca atgccccgtt ctcgttacac acataccgct tttggccgct
       61 gttactgcaa ttggtgttat ccacgatgaa catcgaagta tccggggtgc atcctataac
      121 gcattcgccg gtttcgtcct cccatgcgtt atcgacacag atacggtatc gcacaccggt
      181 gatcagactt tccaccaccg tacaagcgac cgttgatcgg acaccttcat cgcatatctt
      241 ttcctcgcag aacattcctc gacgcacata ctcgtcttca catttccatt cgcaataagc
      301 cggctggctc cacccgcccg ccgaggaata actgacagaa accgtcgacg agaggctctg
      361 tgagttttcc ggtctatccg gatcttcgac gcaagagacc gtttgggtct cgagcgatcc
      421 accgttctcg gatactttga gaaggaagat gtccgcgttg ccggcgctgg tgttgttggg
      481 aagagtacct agcgtatatc cggaggcata cagattatcg gaattgtccc ggaccaatcc
      541 ccacaatcta tccgcatagg ttgatccgtt ctgatatgtc catatcctcg aaccgtccgc
      601 cacatatttc gatatgaaat aatcctcata gcctccatat acattagtgt aaccgtctat
      661 gccgccgcga gtgagtccgc cgacataaat gtttccctgc gtatcgagcg ccaaactggt
      721 cgcctgatcg atcatgccga ccgaggtcgc ctgcatattc acattcggag cgatggtcca
      781 tgtcccgttt tgtccttccg agttataccc ataaaaacga atatagatcg attctcccag
      841 accgaccgat atgctgagtc ccgttatgtt cccgcttcgc gtttctgtat tatcgggcac
      901 cgcaaccgtc gccagatcga ccggattggt gaaagaagac gatttcgaat attgcacttt
      961 gtagttgcgg ataccattgc tcgatcgttt ttccgaaaag ttaattgcgg taacggtcac
     1021 cgcattgccg ggattgggat aaaaatacac ttgataataa tcgtttgaat ccatagcgcc
     1081 ggtcgaccaa ttgttagcgg tcgcgccact cgcattatat gttattgtgc cgatccccgt
     1141 accgcctgaa aataccccgt aaccatatcc agccgaactg taagtggagc cactcgatgt
     1201 caaaggccaa ttgattattt gcacagcggt tctttgcggt gtaccccatt ggctggtcca
     1261 agccaaggtc gccgtcgatc cgtcttgtgt ccatctgctc aagaaagcat cgtatcctcc
     1321 ctgtgctgta ttactaggca aggaacccga cggcagagtc cccgtcgtgt agcccgtcac
     1381 atagagatcc tgatcctgat ttttttccag agaataggca taatcggcgg tgctgctccc
     1441 ccacagtttc gtccatactc ttacggtaga gttgtcatcc gctcctatcg accatttcat
     1501 cagaacaaga tcgtaattgc cgctataagt ctctccgtcc ttttgcgtcc ccgttccgcc
     1561 gtaagaggcg ccggtgatgt acagattatt gttcgaatca atcaaaagat catacccaac
     1621 atcgcccgag gtgtttcccc ataccttcgt ccattccttt gtcgcatccg atccggtatc
     1681 atgccatttg gtgatgaaga tatcttcgcc gccgatcgcg gatttgccat ccaacccgcc
     1741 ggaggttttc cccgtaacat aaatgttgcc gctgccgtct acggtcactg cgttgccgat
     1801 gttggcgctg gcattgcccc atagttttgt ccaagccttg gtaccgtcag gaagatactt
     1861 ggtcaaaaag acgtccgatg aaccggcgtt cgtgttcccg tcgaggtccc cgttcgtgta
     1921 tcccgccgca taaatgttac ctgttccatc gatcgcgacc ccgttcacca catcggtttt
     1981 atcgcttccc cacatacggg tccagaggcg ggtaccgttc ggcatccatt tggtgagaag
     2041 gttatcgcta caaggattac ttccacaggt tgttccgctg gatgtcaccg tttgatcgtc
     2101 cacattgcca ttcgatatgc cgccgacaac gatgttccct tgagggtccg ccgcattcgc
     2161 atagccataa tcgttcagcg tcgatcccca ttgcttgggc catactttgc tcggttcgtc
     2221 ggaaaacttc atgatataga tatcggcaca ggtcgagcca gaacaggagc cgccggcgtt
     2281 cgtgttcccg tccagatcgc cgttcgtagc gccagccaca tacagattcc ccgcatcgtc
     2341 tatggttatg aagcgggttt cttcgtttcc cgcgctaccg cgttgtttca cccatgactg
     2401 gtatccgaac atgtcgtatc gggccacgaa aaggtcggtg ccgccggcat tcgtccctga
     2461 aaacgcgcct gtggtggttc ccactaaata gatatagtcc tcggcgtcga ggatcaaccg
     2521 ggtttccgcc tcattgtccg ccgtaccgaa ctgctgtgtc cactgcacgg ttc
//
LOCUS       DAOQUE010000410         2572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_48529_length_2572_cov_4.625348, whole genome shotgun sequence.
ACCESSION   DAOQUE010000410 DAOQUE010000000
VERSION     DAOQUE010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48529_length_2572_cov_4.625348"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PKH10_11145"
     CDS             <1..718
                     /locus_tag="PKH10_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28717.1"
                     /translation="AFADGAPNPENACEMCDPALDQFGWSDRIENFPCETDDRACTLD
                     VCDGEGTCLHTLYTGCLIGESCVATGVADPTNPCMACDPAVTTDAYSARIDGYPCTED
                     GDDCTYDLCNAGACEHPVKNTLECQSPDDDDIGTDEVVTDEIMTDEMATDDILTDDIL
                     TDETVTDEDAVTVDEQTPDDTTDEEPADDSTVVPDDTVDDEVTDEAVGDEMLTDEDNA
                     LVFPESEAPKDSGCGCSLIF"
     gene            complement(726..>2572)
                     /locus_tag="PKH10_11150"
     CDS             complement(726..>2572)
                     /locus_tag="PKH10_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28718.1"
                     /translation="YPARFALFPIEPPASKALGMGKKYDPDQKIEFTQWDRVARIDVL
                     ANKRSQEFFNYFPELPNKVITIDGDAYTLLYDFPSVSTPGTFDYPRIGLSLYSSAYYT
                     HERPKVLVVGIGGGTDIVTALHHDAASVTAVEINKAMIAATRDHFADLVGRPLQDPRV
                     QVVHSEGRGFIRRSPDRYDILQLSGVDTWTALATGAYVLSESYLYTTDALREYLEHLT
                     NDGTLSIIRWLFWPPRETLRLCTEAVKVLRAMGVAEPHRHIVVIGDGSLAAVLVKPRP
                     FSWHEIALIVEKAAETPRNRIIYAPGFEAPDTYWDPIFEKQAFPPAEGVGHLRASFHR
                     YFEEVAKGTEDRFIAAYDYDITPVTDDQPFFFHYYRLRHLFAADWGGGGSLVDSMPIG
                     LMILALVLVQSLLFSFLLIVAPLFFMSSQERVFSPHLQILYFSMLGIGFMFIEIALIQ
                     QFVLFLGNPSDAIAACVGILLVCAGIGALFSKRVLILFGDRRLYKALALLMPFLVLGY
                     AVLVPWFTHRFLAAPFEARLLLSLLVLFPLGFLMGLFFPTGLTIVGEKNAGFIPWAVA
                     VNGVAGVVSSVLSIVLAMAYGFTFVFTLAAFCYLIAAASFHRFVKKHP"
BASE COUNT          553 a          731 c          861 g          427 t
ORIGIN      
        1 cgccttcgcc gatggcgcgc cgaaccccga gaatgcctgt gagatgtgcg atccggcgct
       61 cgatcagttc gggtggagcg atcggataga gaatttcccc tgcgagaccg acgaccgtgc
      121 ctgcacgctc gatgtctgtg acggcgaagg gacctgcctg cataccctgt acaccggctg
      181 cctgataggg gaaagttgtg tcgctaccgg tgtcgctgat ccgacgaatc cctgcatggc
      241 ctgtgatccg gcggtgacga ccgacgccta cagcgcccgc atcgacggct acccctgcac
      301 cgaggacgga gacgactgca cctacgacct ctgcaacgcc ggcgcctgcg agcatccggt
      361 caagaatacg ctggaatgtc agtcgccgga cgatgacgat atcggcaccg acgaggtggt
      421 gacggatgaa ataatgaccg atgagatggc gaccgacgat atcctgaccg acgatatcct
      481 gaccgacgag acggtcaccg atgaagatgc cgtgaccgtc gacgagcaga cgcctgacga
      541 taccaccgac gaagagccgg ctgacgactc gacggtggtc cccgacgaca cggtcgacga
      601 cgaagtgacc gatgaggccg tgggcgacga gatgcttacc gacgaggaca acgcgctcgt
      661 tttccccgaa agcgaggcgc cgaaggattc cggttgcggc tgttcgttga tcttctagtc
      721 ccttttcacg ggtgcttctt cacgaaacgg tggaaactcg ccgccgcgat cagatagcag
      781 aaggcggcga gcgtgaacac gaaggtgaag ccgtacgcca tcgccagcac tatcgatagg
      841 accgaactca cgacgcccgc gaccccgttg acggcgaccg cccacgggat gaaaccggca
      901 ttcttctcgc ccacgatggt gaggccggtg gggaagaaca gtcccatcag gaacccgagc
      961 gggaagagca ccagtaaaga gaggaggagt cgtgcctcga acggagcggc gaggaaccgg
     1021 tgcgtgaacc acgggacgag caccgcgtag ccgagcacga ggaacggcat gaggagggcg
     1081 agcgccttgt acagccggcg gtcaccgaac aggatgagta ctcttttact gaacagggcg
     1141 ccgatcccgg cgcataccag cagtatcccg acacaggccg cgatggcatc ggaggggttt
     1201 ccgagaaaga ggacgaactg ctggatgagc gctatctcga tgaacatgaa tccgatgccg
     1261 agcatcgaga aatagagtat ttgcaggtgc ggcgagaaga cccgttcctg cgaactcatg
     1321 aaaaagagcg gcgccacgat aagaaggaac gagaaaagaa gcgactgcac gagaacgagc
     1381 gccagtatca tgagcccgat cggcatgctg tcgacgagcg agccgccgcc gccccaatcg
     1441 gccgcgaaca gatgccggag ccggtagtag tggaaaaaga acggctgatc gtcggtgacc
     1501 ggcgtgatgt cgtaatcgta ggcggcgatg aaacggtcct cggtcccctt ggcgacctct
     1561 tcgaagtacc gatggaacga ggcgcgtagg tgtcccacgc cttcggcggg agggaatgcc
     1621 tgcttttcga atatcggatc ccagtaggtg tcgggcgcct cgaaaccggg agcgtagatg
     1681 atgcggttgc gcggtgtttc ggccgctttc tcgacgataa gcgctatctc gtgccacgag
     1741 aagggacgcg gttttacgag caccgcggcg agcgatccgt caccgatgac gacaatgtga
     1801 cggtgtggct cggcgacccc catggcgcgg agcaccttaa ccgcttcggt acagaggcgc
     1861 agggtctcgc ggggcggcca gaaaagccag cggatgatcg acagggtgcc gtcgttggtc
     1921 aggtgttcga gatactcgcg cagagcgtcg gtcgtgtaca ggtaactttc gctgaggaca
     1981 taggcgccgg tcgcgagcgc cgtccaggtg tccacccccg ataactgaag gatgtcgtac
     2041 cggtcgggcg agcgacggat gaagccgcgt ccctcactgt ggacgacctg cacgcgcgga
     2101 tcctgaagcg gtcggcccac gagatcggcg aaatggtcgc gggtcgccgc gatcatcgcc
     2161 ttgtttatct ccacggcggt caccgaagcg gcgtcgtggt gcaatgccgt tacgatatcg
     2221 gtgccgccgc cgatgccgac gacgaggacc ttcggtcgct cgtgggtgta gtaggccgac
     2281 gaatagagcg agagcccgat gcgcggatag tcgaaggtcc ccggcgtgct gaccgacggg
     2341 aaatcgtaca gcagggtata ggcgtcgccg tctatagtga tgaccttgtt ggggagttcg
     2401 gggaagtagt tgaagaactc ctgcgaccgc ttgttggcga gcacatcgat gcgcgccacg
     2461 cggtcccact gggtgaactc tattttctgg tccggatcgt acttcttgcc catgccgagc
     2521 gccttggaag cgggcggttc gatggggaag agggcgaagc gggccgggta ga
//
LOCUS       DAOQUE010000411         2560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_48886_length_2560_cov_4.629940, whole genome shotgun sequence.
ACCESSION   DAOQUE010000411 DAOQUE010000000
VERSION     DAOQUE010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48886_length_2560_cov_4.629940"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PKH10_11155"
     CDS             <1..849
                     /locus_tag="PKH10_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28719.1"
                     /translation="FMETSLAGHYCIGDANGGSGCADQACVDACIAAGTTAAQTQYNA
                     LITCMQSNCAAATECGQGATQQQCNDCMNSKCEDEMDTCFTTDAPVYGTMTAANTAFP
                     FVYDGDSETDLNAQINANQTGFLQTNVFSGNYTAANKAIPPNNATYKIALAYFTASFT
                     ENGNTYPNSVTIMQQHIASDQQTLVNPLVQAWINSDTLGNGQYTIDNGDAGAVLLNDI
                     GNNQACLLAYAFYGTFNITNSSNMTAAGGSFTTNAVGVELYHPTETPQGDISDAFTTI
                     STCPKE"
     gene            1467..2060
                     /locus_tag="PKH10_11160"
     CDS             1467..2060
                     /locus_tag="PKH10_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28720.1"
                     /translation="MKKILSVFAFMSLCSFYLSAFDCGDIWGVQTAQFKKTDYTTGYL
                     NFRFEDSGVGDGSWSEGSDLICLYTYDTPGWPPFINGWPPTEYPSNYLSQIITIRLDP
                     NSQIRLLYSSGNIGDVTLSTTRNKQIWDLVHEDYLYADEYNTTYDSRYWYGDKFYYRL
                     DDALINAVYRVQVKIGFFWYSTSYKIEILQNDSYNWY"
     gene            2186..>2560
                     /locus_tag="PKH10_11165"
     CDS             2186..>2560
                     /locus_tag="PKH10_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28721.1"
                     /translation="MRKVPGIVLFLIVAIFLLCACDGAKTKAPSDLDSTAGGDAATDD
                     DLIGAGCAEGETRCAGELFLRCEGGSFTVTDCAATDATCDPDEGCVSADGDIQTEGSD
                     PAPDEDADAPDCPAHYYRQCDEG"
BASE COUNT          659 a          649 c          598 g          654 t
ORIGIN      
        1 ttcatggaga cctcgctggc cgggcactat tgcattggcg atgcaaatgg cggtagcgga
       61 tgcgctgatc aggcttgtgt agatgcatgt atcgcggccg gcaccactgc ggcccagact
      121 cagtacaatg ccctcattac ctgcatgcag agtaactgtg ccgccgcaac tgaatgcggt
      181 cagggcgcta cgcagcagca atgcaacgac tgcatgaaca gcaagtgcga agacgagatg
      241 gatacctgct ttaccaccga tgcgccggtc tacggcacca tgaccgccgc taacaccgct
      301 tttcctttcg tgtatgacgg agacagcgaa accgatctga atgctcagat caacgcaaac
      361 cagacaggct ttctgcagac caatgttttc tccggcaact acacagcggc aaacaaagcc
      421 attcctccga ataatgccac atacaagatc gctcttgcct acttcacggc atcgtttacc
      481 gagaacggaa acacctaccc caactcggtg accattatgc aacagcatat tgccagtgac
      541 caacagactc tcgttaatcc tctggttcaa gcatggatca actctgacac tttgggcaat
      601 ggccaataca ccatcgataa tggtgatgcc ggagcggttt tattaaatga tatcggcaat
      661 aaccaggcct gcctgctcgc ctacgccttc tacggaacct tcaatatcac caactctagc
      721 aacatgacag cggcgggggg ttcatttact accaatgcag taggtgttga attatatcat
      781 ccaacagaaa ccccgcaagg agacatttcc gacgctttca ctaccatttc gacttgtccc
      841 aaagagtagt tttttagtaa gttccccttt ttttgtatct tcgggccctc cccgcacggg
      901 agggcctttt ttttacacaa aaaaatgaat acccccaact tacataacaa aaaactccaa
      961 aagcgttttt cgtattcgtt gatccggcga gcgaccagtt tcgggtagtc atgttccttg
     1021 agtaagaaag gctacagaaa aaatgaacgg accacaagat gattgctaaa atatattatt
     1081 atccttttat ttcaagtact tgcatgaata ttttagaaca cctgaatctt tttagtttct
     1141 cgccgtatct tatgtatgga cgcagaaatt ttcgagaacg gaacgggaga ggcacctcgt
     1201 gaccggaaat gtcaccgtat gcaataaaca cccttgttcc tccgctttgg gatatttgtc
     1261 atgaactatc catctgttcg atctatcgca agagggactt cgtcgaaatg gcgaagagac
     1321 aggagccgat cacctgtttc ctctgctttt cgaaggccac tatacttttt aaacagaaaa
     1381 acaggactca atgtcgattc tttgttttat tagggaagtg tcatgggaga tgacacttat
     1441 gattaaacat tttgttggag gtcgccatga agaagatctt gtccgttttc gctttcatgt
     1501 ctctctgctc tttctatctt tccgcttttg attgcggaga catttggggg gttcagaccg
     1561 ctcagttcaa aaagacagac tacacaacag ggtatctaaa tttccgtttt gaagactcag
     1621 gtgttgggga tggcagttgg agtgaaggct ctgatttgat atgtctgtat acttatgaca
     1681 ctccggggtg gccaccattt ataaatgggt ggcctccaac ggaatatcct tcaaattatt
     1741 tgtcccaaat aattacgata cgactggatc caaactcaca aattagatta ctgtattcca
     1801 gcggcaacat cggagatgtc actctctcca caaccagaaa taaacaaatc tgggatttgg
     1861 tccatgaaga ttatctgtat gcggacgaat acaataccac ttatgactca aggtattggt
     1921 acggtgacaa attttattat cgccttgatg atgcactgat taacgccgta tatagggtcc
     1981 aagtgaaaat cggttttttt tggtatagca cctcttataa aatcgagata ctgcaaaacg
     2041 attcttacaa ttggtactga ttttactgta ttgctttcgg gccctccccg cacgggaggg
     2101 cctttttttt attcccgtat gccacttgac ttgttcctaa aaagcaataa aatcagccta
     2161 tccgttattt caaagggggt caggtatgcg aaaggttccg ggtattgtcc tgttcctgat
     2221 cgttgcgatc tttctcttgt gcgcctgcga cggcgctaag accaaagcgc ccagcgacct
     2281 cgacagcacg gcgggcggcg acgcggcgac cgacgatgac ctcatcggcg cggggtgcgc
     2341 ggagggagag acccgatgcg ccggcgaact gttcctgcgg tgcgaaggtg gctccttcac
     2401 ggtgaccgac tgcgccgcta cggacgcgac ctgcgacccc gacgaggggt gtgtctcggc
     2461 ggacggcgac atccaaaccg aagggagcga ccccgcccca gacgaggatg ccgacgcccc
     2521 cgactgcccc gcgcactact atcgccagtg cgacgaggga
//
LOCUS       DAOQUE010000412         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_48924_length_2559_cov_3.146965, whole genome shotgun sequence.
ACCESSION   DAOQUE010000412 DAOQUE010000000
VERSION     DAOQUE010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48924_length_2559_cov_3.146965"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PKH10_11170"
     CDS             <1..427
                     /locus_tag="PKH10_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015408017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNT28722.1"
                     /translation="GQMKIAPEHTEDRVLARMGKPSKKVLRRFRELFDALNRKAGKRQ
                     FLTYYFIAAHPGCTEADMQALARFCREELRLSPEQIQVFTPTPSTMAALMYRTGHDPF
                     SGETLFVERSVEGKKRQREVVKGSATLRGAPRSGRGRAT"
     gene            complement(381..2165)
                     /locus_tag="PKH10_11175"
     CDS             complement(381..2165)
                     /locus_tag="PKH10_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28723.1"
                     /translation="MRVISMAFLALFFSVAIAQEPALVPVAPAKKEEPKQEKKEEQKE
                     EQKAEQSNDYQQLYQQYLQKYSTPAETAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPA
                     PVPAPAPAPAPIPAPAPAPMPEPEPVVTPEPEPIATPEPTPAPQPEPKVETPSNFTLS
                     LRTVGQLANGITLFGGDVLKNSYQFDVPQAVIDLKGGTDTFTARLAFDLADGFLTSNT
                     VISPSYDANALTPDVATTVDLVDMIKDISFHFPNIGGYYIEIQGGKFGLPFGVENTRY
                     DHEIPFYWRPLAFEHFLGAGFSDLALQLGANVPISDTMAVKARYFLFNGRNDVQLDGS
                     QYLQDPAHGIDVRFEMKDQFSLIGALSLIIGSAYHDMDTQTAGGIMDTSLDQTDATSG
                     ERFGYQYDVPGQQDFRLYAKDFVRNKMNILLGFGTDLNYKLDENMTFGLAAEFYYSYR
                     QIFNPEKIIDQDGNPATSDLFSYRFLEDASGAFYGQGYYWSYGFFAAPYAHLWDFDAM
                     VRFSMSKQPYLYAFVEDQKNYKLGLDVMLNYNVTDYFQVGIDWRWIQETRNVFDAVID
                     PVNYVKNTYNTHQIMLHALFHYEALLVK"
     gene            2337..>2559
                     /locus_tag="PKH10_11180"
     CDS             2337..>2559
                     /locus_tag="PKH10_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNT28724.1"
                     /translation="MNRKTATATLSVASNTFLIAVKLAVGIVTGSVSVLSEAIHSLFD
                     LIAAIIAFFSVRLSDTPPDDDHPYDHDKIE"
BASE COUNT          502 a          700 c          838 g          519 t
ORIGIN      
        1 gggccagatg aagatcgcgc cggaacatac cgaggaccgg gtgctcgccc gcatgggcaa
       61 gccgtcgaaa aaggtgctcc gtcgcttccg tgaactgttc gacgcgctca accggaaagc
      121 ggggaaacgg cagttcctca cctactattt catcgcggcc caccccggct gcaccgaggc
      181 cgacatgcaa gcgctcgccc ggttctgtcg ggaggaactg cgtctgtcac ccgaacagat
      241 acaggtgttc accccgaccc cttcgaccat ggcggcgctg atgtaccgca ccgggcacga
      301 tccgttcagc ggggaaacat tgttcgtcga acggagcgtc gaaggaaaaa agcgtcagag
      361 ggaggtggtg aaagggagcg ctactttacg aggagcgcct cgtagtggaa gagggcgtgc
      421 aacatgatct ggtgggtgtt gtaggtgttc ttgacatagt tcaccggatc tatcaccgcg
      481 tcgaacacat tccgcgtttc ctgtatccag cgccaatcga taccgacctg gaagtagtcg
      541 gtgacattgt agttcagcat cacatcgagc cccagcttgt agttcttctg gtcctcgacg
      601 aaggcgtaga gatagggctg cttcgacatg ctgaaccgga ccatggcgtc aaagtcccac
      661 agatgggcgt acggggcggc gaagaaaccg taggaccagt agtacccctg tccgtagaat
      721 gcaccgctcg cgtcctcgag gaaacggtag gagaacaggt cggaggtcgc cgggttgccg
      781 tcctgatcga tgatcttctc ggggttgaat atctgtcggt atgaatagta gaactcggcg
      841 gcgagaccga aggtcatgtt ctcgtcgagt ttgtagttga gatcggtgcc gaagccgagc
      901 agtatgttca ttttgttgcg gacgaaatcc tttgcgtaaa ggcggaaatc ctgctgtccc
      961 ggcacatcgt actggtaacc gaaccgttcg ccggaggtcg cgtcggtctg gtcaagcgat
     1021 gtgtccatga tgccgcccgc ggtttgggta tccatgtcgt ggtaggccga accgatgatg
     1081 agcgacagcg cgccgataag gctgaactga tccttcatct cgaaccgcac atcgatgccg
     1141 tgcgccgggt cctgaaggta ctgactgccg tcaagctgga catcgttacg accattgaag
     1201 aggaaatagc gcgcctttac cgccatcgtg tccgatatcg gcacattggc gccgagttgg
     1261 agcgcgagat cgctgaagcc ggcgccgagg aaatgctcga aggcgagcgg gcgccagtag
     1321 aacgggatct catggtcgta gcgggtgttc tcgacgccga aaggcaggcc gaacttgcca
     1381 ccctgtatct cgatatagta gccgccgatg ttggggaagt ggaacgagat atccttgatc
     1441 atgtcgacga gatcgacggt cgtggcgaca tcgggggtca acgcattggc gtcgtaactg
     1501 ggggagatga cggtgttgct tgtcaggaag ccgtcggcga ggtcgaaggc gagccgcgcg
     1561 gtgaaggtgt cggtgccgcc cttgagatcg atgaccgcct gcggcacatc gaactgataa
     1621 ctgttcttga gcacatcgcc gccgaacagc gtgatcccgt tggcgagttg accgaccgtc
     1681 cggagcgaca gcgtgaagtt cgagggggtc tcgaccttcg gttccggttg cggcgccggt
     1741 gtcggttcag gggttgcgat cggttccggt tcgggggtca cgaccggttc aggttccggc
     1801 atgggagcgg gagccggagc aggtatcgga gcgggcgccg gggccggagc cgggaccggg
     1861 gcgggcgccg gggcgggcgc cggagcgggc gccggggcgg gtgccggggc cggagccgga
     1921 gcgggcgccg gagcgggcgc cggggcgggt gccgtctcgg cgggcgtgct gtatttctgc
     1981 aggtactgct ggtacagttg ctggtaatcg ttcgactgtt ccgccttctg ttcctctttc
     2041 tgttcttctt tcttttcctg tttcggttcc tccttctttg cgggggccac cggcacgagc
     2101 gccggttcct gcgctatcgc gaccgaaaag aaaagagcaa ggaacgccat agaaatcact
     2161 cgcatcgttt tcctccttaa caactgtctg acggacgacc gcatgcaccg actgtagtac
     2221 aacggaaaat gaaatacaac atttttttat gtgtccgtgc cggggccgga aatttgattt
     2281 tgcgtacgcc gaaggatacg atggggtgcc acgggaacac ggagcgacgg cggcgcatga
     2341 accggaagac agcgacagcg accctctcgg tcgccagcaa cacctttctc atcgccgtca
     2401 aactggcggt gggtatcgtc accgggtcgg tgagcgtcct gtcggaggcg atacactcgc
     2461 tgttcgacct catagctgcg atcattgcct ttttcagcgt tcgtctctcc gacacgccgc
     2521 ccgatgacga ccatccctac gatcacgaca agatagagg
//
LOCUS       DAOQUE010000413         2558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_48951_length_2558_cov_4.290451, whole genome shotgun sequence.
ACCESSION   DAOQUE010000413 DAOQUE010000000
VERSION     DAOQUE010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48951_length_2558_cov_4.290451"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PKH10_11185"
     CDS             <1..600
                     /locus_tag="PKH10_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28725.1"
                     /translation="EIPEPAHNQLNKSNPVLHIRAKSLDGDNQLRSIRVKTVGDSVDF
                     GAGMQAITIWEDANGDGVGDTLLVTSGTFSEPTNDYSFDDLQSGAAFQFADKQERYYT
                     INITFNLESAMTAQIEIPNLGVKLMDSAKKILELPIRSRSYQCAEPFCYAPPSKGCSC
                     AFVDIGDEDDGVLLTALMVVISLGLFFGLRRGNRKASRA"
     gene            720..2228
                     /locus_tag="PKH10_11190"
     CDS             720..2228
                     /locus_tag="PKH10_11190"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNT28726.1"
                     /translation="MLLSVCLWGQTRNSKLAVMEIGDKTGKMTPEMLDNAAEYLRSKL
                     VSSGRFVVISKERQMAVRIKELKKESYKECYDQKCQIPLGQALAADTILSTTITKMSG
                     IYVITAELIDLSKEATIFGATAQFDGLEEGLRRALDAIADQLQNARPVMPATGKAVVY
                     AIKFEANAPGAKAYLDGRLFCFNLPCEKDVEEGEREIRITAPGYVAEEFKTLIDKSRV
                     FSAQMKKDEAFLTITNTDLIGDMAIQFDGAAIEGFPMPKRQVAPGKHTLVVESACYEK
                     LDGVIELNPEEEKEIKVGLIPKKVSLSVEVTDSGGAKLEGAQVSVDGVVVGNAPGPFS
                     VPICSKGLVIRKPGYVDFWLPLAFKEGEPFNAVATLDYQYETIRPYKWWGVGLLAGGA
                     AVLGVGIAFDVLADKEYRDYRTMSMPAALEEAMKEPDFTRSSYDEKMKKVYDRAKIYD
                     ALRITGYVVGGAMAVTGVVLMAIPEEVKVLDLEGFSVAPYRDGAMLSFGYEF"
     gene            2237..>2558
                     /locus_tag="PKH10_11195"
     CDS             2237..>2558
                     /locus_tag="PKH10_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28727.1"
                     /translation="MKKILLTTLLLLATACARDYGDLTETTHKNIDKIVGWGAICENG
                     TVRDVACGFNNRGARPQKCESGAWVDNGDCVDPDDCLDDDTRVVERVTCLDTIGDQMQ
                     KCIEG"
BASE COUNT          580 a          670 c          792 g          516 t
ORIGIN      
        1 gagataccgg aaccggcgca caatcagttg aacaagagta atccggtcct ccacatccgc
       61 gccaagtcgc tggatggcga taaccaactc cgctccatcc gcgtcaagac ggtcggcgac
      121 tcggtcgact tcggcgcggg gatgcaggcc atcaccatct gggaggacgc caacggcgac
      181 ggcgtcggcg atacccttct cgtgacatcg ggtaccttca gcgagccgac gaacgactat
      241 tcgttcgacg acctccagtc cggtgccgca ttccagttcg ccgacaaaca ggagcgctac
      301 tacacgatca acatcacctt caacctcgaa tcggccatga cggcgcagat agagataccg
      361 aacctcggcg tgaaactgat ggacagcgcc aagaagatac tggagttgcc catccgctcc
      421 cgttcctacc agtgcgccga gccgttctgc tacgcgccgc cctccaaagg ctgttcgtgc
      481 gccttcgtgg atatcggcga cgaggacgac ggcgtgctcc tgacggcctt gatggtcgtg
      541 atctctctgg ggcttttctt cgggctccgg cgcggcaata gaaaggcgag ccgcgcctga
      601 ggggggatct tcccgccgcc ccgttttctt gttttttctt tccgttccgc taaaatcgct
      661 tcgttatctc actgttcgag gggtttctta tgtctgcgcg atccacgatc ttgattctca
      721 tgcttctctc ggtctgtctc tggggccaga cgcgcaacag caaactggcg gtcatggaga
      781 tcggcgacaa gacggggaag atgacccccg agatgcttga caatgccgcc gaatatctgc
      841 ggagcaaact tgtttcatcg gggcggttcg tcgtcatttc caaagagcgg cagatggcgg
      901 tcaggataaa ggagttgaaa aaggaatcgt acaaggaatg ttacgaccag aaatgccaga
      961 taccgctcgg gcaggcgttg gcggccgaca cgatactttc aacgacgata acgaagatga
     1021 gcggcatcta tgtcatcacc gccgaactca tagacctctc caaggaggcg accatcttcg
     1081 gagcaacggc tcaattcgac gggctggaag agggactgcg tcgtgcgctt gatgcgatag
     1141 cggatcaatt gcagaatgcc cgtccggtca tgccggcgac cgggaaggcg gtggtgtacg
     1201 ccatcaagtt cgaagcgaac gccccggggg caaaggccta cctcgacggg cgcctattct
     1261 gcttcaatct gccgtgtgag aaggatgtcg aagagggtga gcgtgagata cggataaccg
     1321 ctcccggata tgtcgccgag gagttcaaaa ccctcataga caaaagtcgc gtcttctcgg
     1381 cgcagatgaa aaaggacgag gccttcctca ctataacgaa caccgacctc atcggcgata
     1441 tggcgataca gttcgacgga gcggctatag aagggttccc aatgccgaaa cgacaggtgg
     1501 cccccgggaa acatacgctc gtcgtcgaat cggcctgcta tgagaaactc gatggggtca
     1561 tcgaactcaa tccggaggaa gaaaaggaga taaaggtcgg cctgattccc aagaaggtgt
     1621 cgctttcagt agaagtgacc gacagcggcg gcgcgaaact cgaaggggcg caggtgtcgg
     1681 tggatggcgt ggtggttggt aatgcaccgg gacccttttc ggtgccgatc tgctcgaaag
     1741 gattggtgat tcgcaagccg ggctatgtgg atttctggtt gccgcttgcg ttcaaggagg
     1801 gagaaccctt caatgccgtt gccacgctcg attaccagta tgagaccatt cgcccctaca
     1861 agtggtgggg cgttggcctt cttgccggtg gcgccgcggt gcttggcgtc ggtatcgcgt
     1921 tcgatgttct tgccgacaag gaataccgcg actaccggac catgtcgatg ccggcggcac
     1981 tcgaagaggc aatgaaggaa cccgacttca cgcggagctc ctacgatgag aagatgaaaa
     2041 aagtgtacga cagggcgaag atatacgacg ccctccgcat cacagggtat gtggtgggcg
     2101 gcgcgatggc ggtcaccggg gtcgtgctga tggcgatacc ggaagaggtg aaggtgctcg
     2161 acctcgaagg gttctccgtc gcgccgtacc gtgacggggc gatgctttcg ttcggatatg
     2221 agttttaggg gggggcatga agaaaatact gttgacaacg ctcctccttt tggcaacggc
     2281 gtgcgcaaga gattatggtg acctgaccga gacgacccac aagaacatag ataagatcgt
     2341 tggatgggga gcgatctgtg agaacgggac ggtgcgggat gttgcttgcg gatttaacaa
     2401 tcgtggtgca agaccgcaga aatgcgaaag tggcgcatgg gttgacaatg gtgattgtgt
     2461 cgaccccgat gattgtctgg atgatgacac gcgagttgtt gaaagagtaa catgcttgga
     2521 tacgataggc gaccagatgc agaagtgtat cgaggggc
//
LOCUS       DAOQUE010000414         2553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_49095_length_2553_cov_3.829464, whole genome shotgun sequence.
ACCESSION   DAOQUE010000414 DAOQUE010000000
VERSION     DAOQUE010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49095_length_2553_cov_3.829464"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2370)
                     /locus_tag="PKH10_11200"
     CDS             complement(<1..2370)
                     /locus_tag="PKH10_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28728.1"
                     /translation="MSGYDAAGNELTTMPLGSVMIDVKPPDVSTYDLSHTHVRLGEQF
                     TLSFTVTEELVSAPVVLVGAKNISDECVADGLDYTCAHTATTDEGDGVKQLALQLTDL
                     AGNNTNRTLKDTADQYVTINYDVTAPTVVNPVVAPEKANLGATVDVRFSFTEDVTGVT
                     IDWGLLDGLFTRDPSNEGNARLFIYKRQITDTDPQGEFAITVTAATDLAGNPITAAIP
                     IGSLEIDRKAPEAKMPAVAVNGDTGKTLAKEGDTVTVSFTVEEEIQEYKVRIGLIPVS
                     ACTFDDDEQRYTCTHTVQESDAEGTKDVTVELKDLAGNADTYPLGQVSFDMTEAELAN
                     AIILPTKSNKATVKVEVKFSFTEEVVMTALADGGLGLSGADCPLNGDPRRDFICDHAF
                     DIDDTQVNDYPVTVSANDLAGNPSIDLVVGTVSVDRAIPSLLAQDVAPTTLKLGDQFT
                     ISFTSSETLEGDPIVKVGSLQLGAPTTHDGNDYTYTHTAGAGEGDGDKLIAVTLTDPA
                     GNGRTVDLTKTVTYDTTAPTVLNPTVSPETANRQTETIDVRFSFPEDVEAVTLTGLKL
                     SGDVDAPASADFTCVEQGSAKSWKCSVSLVLISWEIAEYELWVNGRDLAGNYINPADE
                     TNGQTLGLNAVIDRKRPVISGETVTQCTGIDCNTAKTVSKAGDAVRVSFEIDETPLAE
                     PVVGIAGRLATACPGPKTYNYCIVVQSDDGDGEKDIRIETADAAGNIAVKTLAVKPLF
                     DVTAPKVTNPVISPNPAAATSVASLSFSFSEPIPVAQMSVTSTSGQATPF"
     gene            complement(2276..>2553)
                     /locus_tag="PKH10_11205"
     CDS             complement(2276..>2553)
                     /locus_tag="PKH10_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28729.1"
                     /translation="PGRAGTGGKISVVFTASEPLTTTTFDNDGLVFENSVSLDGLTHT
                     YTYGVTDRRGTDLRFLGERLRRGRQRTDNDAARQRHDRCQTSRCLHL"
BASE COUNT          435 a          740 c          813 g          565 t
ORIGIN      
        1 gaacggggtc gcctgacctg aggtcgaggt gacgctcatc tgcgcgacgg gtatcggttc
       61 gctgaagctg aacgagagcg aggcgacact cgtcgcggcc gccgggttcg gcgatatgac
      121 cgggttggtg accttcgggg cggtcacatc gaagagcggc ttgacggcaa gcgttttgac
      181 ggcgatgttg cccgccgcgt cggcggtctc tatccgaata tctttttctc cgtcaccgtc
      241 atccgattgg acgacgatgc agtagttgta ggtcttcgga ccgggacaag cggtcgcgag
      301 acggcccgcg atacccacca ccggctctgc aaggggcgtt tcgtctatct cgaaggaaac
      361 gcggaccgca tcgcccgcct tcgataccgt tttagcggtg ttgcaatcga tccctgtgca
      421 ctgggtgacc gtctctcccg atatcaccgg ccgtttccgg tcgatgacgg cgttcagccc
      481 gagagtctgc ccgttcgtct cgtcggccgg gttgatgtag ttcccggcaa gatcacgacc
      541 gttgacccac aactcgtatt cggctatctc ccacgagatc agtacgagcg acacggaaca
      601 tttccaactc ttcgcgcttc cctgttcgac gcaggtgaaa tcggcggatg cgggggcgtc
      661 gacatcgccg gaaagtttga gaccggtgag cgtcaccgcc tcgacatctt cgggaaagct
      721 gaaccggaca tcgatcgtct cggtctggcg gttggccgtt tcgggcgata cggtcgggtt
      781 gagcaccgtc ggagcggtgg tgtcgtaggt caccgtcttg gtcagatcga ccgtccgacc
      841 gttgcccgcc ggatcggtga gggtcaccgc gatcagtttg tcgccgtctc cctcacccgc
      901 tccggcggta tgggtatagg tgtaatcgtt cccgtcatgg gtggtcggtg cgccgagttg
      961 caaagagccc accttcacga tgggatcgcc ctcgagcgtt tccgatgagg tgaaggatat
     1021 ggtgaactgg tcgcctagtt tcagcgtcgt cggcgcgaca tcctgcgcga gaagcgacgg
     1081 tatcgcgcgg tctaccgaca cggtgcctac gacaaggtcg atcgatgggt tgccggcgag
     1141 gtcgttcgcc gaaacggtga cgggataatc gttcacctgc gtgtcgtcga tatcaaaggc
     1201 gtgatcacag atgaagtcgc gccgcggatc gccgttcagg ggacagtcag cgccggaaag
     1261 gccgagcccg ccgtcggcga gcgccgtcat gacgacctct tcggtgaagg agaacttgac
     1321 ctcgaccttc acggtcgcct tattgctttt cgtcggtagt atgatggcgt tggcaagttc
     1381 cgcttcggtc atgtcgaagg agacctgtcc gagcgggtag gtgtcggcat tgccggccag
     1441 gtccttcagt tccacggtga catctttcgt cccttcggcg tcgctttctt gaacggtatg
     1501 ggtgcaggtg taccgctgtt catcgtcatc gaaggtgcag gcagagacgg gtatgagccc
     1561 gatgcgaacc ttgtattcct gtatctcctc ctccacggtg aaggagacgg tgacggtgtc
     1621 accctccttc gcaagcgtct taccggtgtc gccgttcacc gcgacggcag gcatctttgc
     1681 ctcgggagct ttccgatcta tctcgagcga acctatcggt atcgccgccg ttatcgggtt
     1741 gcccgccaga tcggtcgccg cggtcaccgt tatcgcgaat tccccttgcg gatcggtgtc
     1801 ggttatctgt cgcttgtaga tgaacagacg agcgttgccc tcgttgctcg ggtcgcgggt
     1861 gaacaatccg tcaagaaggc cccagtcgat ggtcacgccc gtcacatcct cggtgaagga
     1921 gaagcggaca tcgaccgtgg ccccgagatt cgccttctcg ggtgcgacga ccgggttcac
     1981 gacggtggga gccgtgacat cgtaattgat ggtcacatat tgatcggccg tgtctttcag
     2041 cgtccgattg gtattgttgc cggcgaggtc cgtcaactgc agggcaagct gtttcacgcc
     2101 gtcgccttca tcggtggtcg ccgtgtgcgc gcaggtgtag tcgagcccat cggctacaca
     2161 ttcgtctgat atgttcttgg cgccgacaag caccaccggg gccgacacca gttcctcggt
     2221 caccgtgaat gagagcgtga actgctcgcc aagccggaca tgggtatgcg agaggtcata
     2281 ggtggagaca tcgggaggtt tgacatctat catgacgctg ccgagcggca tcgttgtcag
     2341 ttcgttgccg gccgcgtcgt agccgctcac cgagaaatcg taggtccgtt cctcgtcggt
     2401 cggtcacgcc gtaggtgtag gtgtgggtga gtccgtcgag cgacaccgaa ttctcgaaga
     2461 caagtccgtc gttatcgaag gtggtcgtcg tcagcggctc gctggcggtg aagaccaccg
     2521 atatcttgcc gccggtccca gcccgacccg gtg
//
LOCUS       DAOQUE010000415         2547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_49291_length_2547_cov_4.061798, whole genome shotgun sequence.
ACCESSION   DAOQUE010000415 DAOQUE010000000
VERSION     DAOQUE010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49291_length_2547_cov_4.061798"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..406
                     /gene="rpsK"
                     /locus_tag="PKH10_11210"
     CDS             26..406
                     /gene="rpsK"
                     /locus_tag="PKH10_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNT28730.1"
                     /translation="MAEQKKKKVRKNIQTGLAYVQCSFNNTIVTITDVSGNVLSWSSA
                     GLKGFRGSKKSTPYAAQMAAEDAGKKAKEYGLRTLSVYVKGPGNGREAAIRGLGNAGY
                     NIMLLRDITPIPHNGCRPAKRRRV"
     gene            452..1081
                     /gene="rpsD"
                     /locus_tag="PKH10_11215"
     CDS             452..1081
                     /gene="rpsD"
                     /locus_tag="PKH10_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNT28731.1"
                     /translation="MAKYKESSCKICRKEGAKLFLKGERCHSEKCAYDRRPYGPGQHG
                     KDRKRTTDYRVQLREKQKVKFMYGLLESQFRLFYDRAVKKKGATGENLLNLLERRLDN
                     VVFRLGMASSRAQARQLISHRHFLVNGKITDIASFIVKQGDVIQIKEASRTTPVFKAS
                     LEYAGERVQVPTWLQLEKEQFKGSVLREPERTDISADIKENLIVELYSK"
     gene            1099..2121
                     /locus_tag="PKH10_11220"
     CDS             1099..2121
                     /locus_tag="PKH10_11220"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNT28732.1"
                     /translation="MYRQNWKTLIMPKEVKVVEQNEAGTFARFICEPLMKGYGITLGN
                     SLRRILLSSIRGAAIVAVKIKGVEHEFSTIPGVKEDVVNVFLNLKQIRFVAHEEKMHH
                     LVLRRKNKGMVTAGDIPETAALKILNKDQVLFEMDENVDVEMELLVMSGRGYVPAEDH
                     DPSLYSEGFIPIDSFFSPVVRVSYAITGARVKNNFNYDKLTIEVETDGAMHPRDALAY
                     ASMILREHTRFLINFAIEEETEGKEGDLSEETDWNLYKTIDDLDLSVRSYNCLKSANI
                     RFVGDLVKKSEAEMLKTKNFGRKSLNEIKLILLNMGLYFGMELPNFPDQRILDQIEKK
                     KEKEGK"
     gene            2133..>2547
                     /gene="rplQ"
                     /locus_tag="PKH10_11225"
     CDS             2133..>2547
                     /gene="rplQ"
                     /locus_tag="PKH10_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNT28733.1"
                     /translation="MRHKKDNKRLGRPTEHREAMLTNLLQSLIKHGRITTTLVRAKEL
                     QRLADRVVTIAKKRSHAAYVQLTKYIKSERELLIPKLMEEIAPKFAERKGGYTRVVKL
                     GFRRGDAAPTAIIEYLYEVLTGKKLEEEASGEKKPA"
BASE COUNT          657 a          647 c          768 g          475 t
ORIGIN      
        1 aaaaagagct agagagggat agaccatggc cgaacaaaag aagaagaagg tccggaagaa
       61 tatccagacg gggctggcgt atgtccaatg ttctttcaac aataccatcg ttaccatcac
      121 cgatgtgagc ggtaatgtgc tcagctggtc gagcgcgggg ctcaagggtt ttcgcggttc
      181 caagaagagc actccctacg ccgctcagat ggcggccgag gatgccggca agaaggcgaa
      241 agaatatggc ttgcgcacgc tcagtgtgta tgtgaagggt ccgggcaacg gtcgcgaggc
      301 ggccatccgc ggactcggca atgccggata caacatcatg ctcctccgtg acatcacgcc
      361 gatcccgcac aatgggtgcc ggccggcgaa acggagaaga gtatagttgt cggaagtgta
      421 aacttttaat cgaaggtaaa aggaggaagc tttggcgaag tacaaggaaa gcagttgtaa
      481 gatctgtcgc aaggaagggg cgaaactttt cctgaaggga gagcggtgtc attccgagaa
      541 gtgcgcctat gatcgtcgcc cctacggccc cggtcagcac ggcaaggatc gtaaacgcac
      601 caccgattat cgggtccagt tgcgcgagaa acagaaggtg aaattcatgt acggtcttct
      661 ggaaagccag ttccggctgt tctacgaccg tgccgtcaaa aagaaagggg ccaccggcga
      721 gaatctgctc aatctgctcg agcggcgcct ggacaatgtg gtgttccgtc tgggcatggc
      781 atcgagccgt gcgcaggcga ggcaactcat cagccatcgc cattttcttg tcaacggtaa
      841 gatcaccgat atcgcgtcgt tcatcgtcaa gcagggcgat gtcatacaga taaaggaagc
      901 cagcaggacg accccggtgt tcaaggcgtc cctggaatac gccggggagc gggtgcaggt
      961 cccgacatgg ctccaactcg agaaggagca attcaagggg tcggtgcttc gggaacccga
     1021 acggaccgat atctcggccg acatcaagga gaatcttatc gtcgagcttt attccaagta
     1081 aactgcgagg tgcccgggat gtatcgtcag aactggaaaa cgcttattat gccgaaagag
     1141 gtgaaggtgg tcgagcagaa cgaagcgggc acctttgccc gattcatatg cgagccgctc
     1201 atgaaggggt atgggatcac gctggggaac tctctgcgga ggatattgct ctcctcaata
     1261 cgcggagcgg cgatcgtggc ggtgaagatc aaaggggtgg agcacgagtt ctcgaccatc
     1321 ccgggagtta aggaggatgt ggtcaatgtc ttcctcaatc tcaagcagat ccgtttcgtc
     1381 gcgcacgagg agaagatgca tcatctggtc ctgcgtagga aaaataaggg tatggtgacg
     1441 gccggcgata tccccgaaac ggccgctctc aagatactca acaaggatca ggtgcttttc
     1501 gagatggatg agaatgtcga tgtcgagatg gaactgctcg tgatgtcggg acgtggctat
     1561 gttcccgccg aggatcacga tccctctctg tacagcgagg gctttatccc catagattcg
     1621 ttcttctccc cggtggttcg cgtaagttac gctatcaccg gcgcccgcgt gaagaacaac
     1681 ttcaattacg acaaactgac catcgaggtg gagaccgacg gcgcgatgca tccgcgcgat
     1741 gcgctggcgt atgcgtcgat gatcctgcgg gagcacacac gcttcctcat caatttcgcc
     1801 atagaggaag agaccgaagg caaggagggc gacctgtccg aagagaccga ttggaatctc
     1861 tacaagacga tagacgacct cgatctctct gtccgatcct acaattgcct gaagagcgcc
     1921 aatatccgtt tcgtcggcga tctggtgaag aagagcgagg cggagatgct caagacgaag
     1981 aatttcggcc gcaagtcgct gaacgagata aaactcatac tcctcaatat gggactgtac
     2041 ttcgggatgg agttgccgaa cttccccgat cagcgcatcc ttgatcagat agagaagaag
     2101 aaagaaaaag aaggcaagta agggagacca tcatgaggca taagaaagac aacaaacggc
     2161 tgggacgccc gacggaacac cgcgaggcga tgctgacgaa cctgttgcag tcgctcatca
     2221 agcatggccg catcacgacg acgctcgtcc gcgcgaaaga gttgcaacgg ctcgccgacc
     2281 gggtcgtgac catcgcgaag aagcgttcgc acgccgccta tgtgcaactc acgaaataca
     2341 tcaagtcgga acgcgaactc ctgatcccga aactcatgga agagatcgcg ccgaagttcg
     2401 ccgaacggaa aggcgggtac acccgcgtgg tgaaacttgg tttccgccgc ggcgatgccg
     2461 cgcccaccgc catcatcgag tacctgtacg aggtgttgac gggtaagaaa ctggaggaag
     2521 aggcgtccgg ggaaaagaag ccggcga
//
LOCUS       DAOQUE010000416         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_49350_length_2545_cov_3.929719, whole genome shotgun sequence.
ACCESSION   DAOQUE010000416 DAOQUE010000000
VERSION     DAOQUE010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49350_length_2545_cov_3.929719"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PKH10_11230"
     CDS             complement(<1..513)
                     /locus_tag="PKH10_11230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNT28734.1"
                     /translation="MQFDWMKIPYALLTLFIVINFGYLLNSFIAKIAARVGRRRGIPI
                     WQTYIDVAKNYGLRTSITHGVMFYLGPVFRLSGGVGLLLFVPSIYGSYMFSNLSFAGD
                     LILALYFVFFGTLGMALGAAESGHPYSAIGITRGLSQVTAAELPFALAVFSVALQYQT
                     LSVTDIVAAQQ"
     gene            complement(515..>2545)
                     /locus_tag="PKH10_11235"
     CDS             complement(515..>2545)
                     /locus_tag="PKH10_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNT28735.1"
                     /translation="VSFVALLTGLPPFPGFYAKWDLAVALAANGRYMILAFILLGALI
                     EAGYLFRWFGYALKRDKPAEEISCYPLAVTAVVAALAAGWALGYFWGDLSAHGNILHA
                     VPLLFALSFLIIDWLPAWVKNTIAIAGMVGYFILNYAAYDPLRLIFAIIILIGGPLIL
                     LASYDAHGRRVGFYPAAMLMYAGLTQLIGATDFFSFFVAWELLTVGSYFLILRGKHSE
                     PHALSYITFSLGGAFLILTGFALASAVSPLPMFDALKHLTGETAGWVFLLLAVGFMTK
                     TAAIGLHIWLPGAHAEAETDVSPMVSGILLKAGLYGLMVLMLAMGKQRFYGVELTHVM
                     LWVGALSALLGNLMAIFEEDAKRLLAYSSIGQMGYALFGLALFNHLGWMMALMFVINH
                     YIYKSMLFLSAGGVAKRTGTRLMYKMGGLITLMPLSFIAVLIGIIAVSGVPPLSGFGG
                     RWIFYNAILTAEHRLPMIITFFSGPIAFLYLFRLIHTMFLGQLKDENRRIKEAPFWVI
                     LPQMLYVGLLMAFAIVPGLALRRVDAYLNQFFPEGGLNWEGLTITSQFGYWSPVSIMI
                     IVCVIFGSLFAWLLFINRRAQKVKQFNIVFSAERPYRPETTHFAWNFFAPYRKALGFL
                     VQPFVTRFWATVTDTLHAVADLSRRMYTGNGQTYALQFLAFVVIVYLFRIGG"
BASE COUNT          561 a          674 c          917 g          393 t
ORIGIN      
        1 ctgctgggcc gcgacgatat cggtgaccga aagggtctga tactggagcg ccaccgagaa
       61 gacggcgagc gcgaagggga gttctgcggc ggtcacctgc gacaggcccc gcgtgatgcc
      121 gatggcggag taggggtggc cgctttcggc ggcgccgagc gccatcccga gcgtcccgaa
      181 gaagacgaag tagagggcga gtatgaggtc gcccgcgaac gagaggttcg agaacatgta
      241 ggaaccgtag atcgacggga cgaacaggag cagccccacg ccgcccgaga gtcggaacac
      301 cgggccgagg tagaacatca cgccgtgcgt tatcgaggtg cgcaggccgt agttcttggc
      361 cacatcgatg taggtctgcc agatcgggat gccgcgccgc cgcccgaccc gcgccgctat
      421 cttggcgatg aatgagttga gcaggtagcc gaagtttatg acgatgaaaa gggtgaggag
      481 cgcatagggt atcttcatcc agtcgaactg catcctaccc tccgatccgg aaaagataga
      541 cgatcacgac aaaggcgagg aactggagcg cgtaggtctg tccgtttccg gtgtacatgc
      601 gccgcgacag atcggccacg gcgtgcagag tatcagtgac cgtcgcccag aagcgggtca
      661 caaagggctg tacgaggaag ccgagcgcct tgcggtaggg ggcgaagaag ttccacgcga
      721 agtgggtcgt ctcggggcgg tagggccgtt cggccgaaaa gacgatattg aactgcttga
      781 ccttctgcgc gcggcggttg atgaagagga gccacgcgaa gagcgatccg aatatcacgc
      841 agacgatgat catgatcgag acggggctcc agtagccgaa ctgactcgtt atcgtaagtc
      901 cttcccagtt cagcccgcct tcggggaaga actggttgag ataggcgtcg acgcgccgca
      961 gggcgagccc cgggacgatg gcgaaggcca tgaggaggcc gacatagagc atctgcggca
     1021 ggatgaccca gaacggcgcc tctttgatgc ggcggttctc gtctttaagt tgtccgagga
     1081 acatcgtatg gatgaggcgg aagaggtaga ggaaggcgat cggtcccgag aaaaaggtga
     1141 tgatcatcgg cagacggtgt tcggccgtga ggatggcgtt gtagaatatc caccggccgc
     1201 cgaagcccga cagcggcgga acgcccgata cggcgatgat gccgatgagg accgcgatga
     1261 aggagagcgg catgagagtg atgaggccgc ccattttgta catgagccgc gtgccggtcc
     1321 gcttggcgac gccgcccgcc gagaggaaga gcatcgactt gtaaatgtaa tggttgatga
     1381 cgaacataag cgccatcatc cagccgaggt ggttaaagag ggcgagcccg aagagggcgt
     1441 agcccatctg gccgatggag gagtaggcga ggagccgttt ggcgtcctcc tcgaagatcg
     1501 ccatgaggtt gcccagtagc gccgagagcg cgccgaccca gagcatgaca tgggtgagtt
     1561 cgacgccgta gaaccgttgt ttccccatcg cgagcatcag caccatgagt ccgtagagac
     1621 ccgctttgag caggatgccc gacaccatcg gcgagacatc ggtctcggcc tcggcgtggg
     1681 cgccgggaag ccagatgtgg aggccgatcg cggcggtctt ggtcatgaag ccgaccgcga
     1741 gcaggaggaa cacccatccg gccgtctcgc cggtcaggtg tttcagggcg tcgaacatcg
     1801 gcagggggct gacggcggag gcgagcgcga agccggtgag tatcaggaac gcgccgccga
     1861 gcgagaatgt aatataggaa agggcgtgcg gttcggaatg tttgccgcgc agtatcagga
     1921 agtaggagcc gacggtgagc agttcccacg cgacgaagaa cgagaagaag tcggtcgcgc
     1981 cgatgagttg ggtaagcccg gcatacatca gcatcgcggc agggtagaag ccgacgcgcc
     2041 gtccgtgggc gtcgtaactc gccagcagta tcaacggccc gccgatgagg atgatgatgg
     2101 cgaagatgag ccggagcggg tcgtaggcgg cgtagttgag gatgaagtag ccgaccatcc
     2161 cggcgatggc gatggtattt ttcacccacg ccgggagcca atcgatgatt aggaacgata
     2221 gcgcgaacag gagcggcacg gcgtggagga tgttgccgtg cgccgagagg tcgccccaaa
     2281 agtagccgag cgcccagccg gcggcgagcg cggcgacgac cgcggtcacg gcgagcgggt
     2341 agcaggatat ctcttctgcg ggcttgtcgc gtttcagcgc gtagccgaac cagcggaaga
     2401 ggtagcccgc ctcgatgagc gcgccgagca gtatgaaggc gaggatcatg taccggccgt
     2461 tcgcggcgag tgcgacggcg aggtcccact tggcgtagaa gccggggaag gggggtaggc
     2521 cggtcagaag cgcgacgaac gagac
//
LOCUS       DAOQUE010000417         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_50117_length_2521_cov_4.017437, whole genome shotgun sequence.
ACCESSION   DAOQUE010000417 DAOQUE010000000
VERSION     DAOQUE010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50117_length_2521_cov_4.017437"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /gene="amrS"
                     /locus_tag="PKH10_11240"
     CDS             <1..691
                     /gene="amrS"
                     /locus_tag="PKH10_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNT28736.1"
                     /translation="LTPAQVVTLAKKYGSDSISFTYTEPAVFFEYMRDIAKLAREQGL
                     RTILVSCGYLNEKPLRELANHLDAANIDLKGFTDEFYRTYTTARLQPVLDTLKVLKQE
                     RVHIEVTNLVIPGANDTENEMRELARWVKENLGADTPLHFSRFFPKYKLTARPPTPPV
                     TLEKARAIALKEGARFVYIGNLATDDGEDTFCPKCGKKLVDRGGYHIDAFLLKDGRCP
                     FCGETINGRWR"
     gene            692..>2521
                     /locus_tag="PKH10_11245"
     CDS             692..>2521
                     /locus_tag="PKH10_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28737.1"
                     /translation="MRAWLDPALFFALVLGLVTALSQTVLIRELMLATGAFEMVVAVG
                     LSLWMLFTGVGAVASGMFPVLRRPFVPPVLVALVAIVAVGQLFLVHPLAALFGMVGGM
                     ALSVPAMVVVTAIVLAPGCLVGGLAFPACVAAVRGGAERTGLVYLIESGGMALGAALF
                     YFFMEFFSPVRHPIFFAHYQERYRPEELMLSRDGRSGRLDVTRRGDRIAYFWNGQAAG
                     VVGPNRPAEAFAGLALTQRPDPRRIAVIGGLLAGAAAEVARKSPEAVVTVIESEPMFA
                     EVFADGTVGRNGALRFGDPGAVLRELPPQDLIIIDLPDPSSVSLSRFFSREFFSFLQE
                     DNGPAMVLVVGLSGGQGMLTPETASLNRSVQLALRAVFRKVLLLPASRHLWVAAENDL
                     PTADPVFIEERMERSGLAGDWFNMALVRDVLEPMHRELTGAAIERADARENRLLRSSA
                     LFETLRHTARRLDRPLSSLFSSLPSRPFSYLGVTLLFAAAVAIFLSRLTRRRLAPARS
                     AAIFAVSGGAFTLQLALMALLQLHVGQIYHLIAIFTVSFTVGLTAGLWVSRRVALPLN
                     LSTGALALVSLATAWGADSGTGAAFPVAMNLLAGLTAGLSFGTL"
BASE COUNT          378 a          814 c          796 g          533 t
ORIGIN      
        1 cctgaccccg gcacaggtgg tgacgctcgc gaaaaaatac gggagtgaca gcatatcgtt
       61 cacctacacc gagccggcgg tctttttcga atacatgcgt gacatagcga aactcgcccg
      121 tgaacagggc ctgcggacga tactcgtctc gtgcggctac ctgaacgaga agccgctccg
      181 cgaactggcg aaccaccttg acgccgcgaa catagacctc aaaggcttca ccgacgagtt
      241 ctaccgtacc tatacgaccg cgagactcca gccggtcctc gatacgctga aggtgctgaa
      301 gcaggagagg gtgcacatcg aggtgaccaa tctcgtcatc ccgggcgcca acgatacgga
      361 gaacgagatg cgggaactgg cacgatgggt gaaggagaac ctcggcgctg atacgccgct
      421 ccacttctcc cgttttttcc cgaaatataa actgaccgcg cgacctccga cgccgcccgt
      481 gacgctggag aaagcgcgcg ccatcgcgct gaaggagggg gcgcggttcg tctatatcgg
      541 caatcttgcg accgatgacg gcgaggatac cttctgtccg aagtgcggga aaaaactcgt
      601 cgaccggggc ggttaccata tcgacgcctt tctgctgaaa gacgggcgct gtcccttctg
      661 tggcgaaacg ataaacgggc ggtggcggta agtgcgcgca tggcttgatc ccgctctctt
      721 cttcgccctg gtgctcggac tggtcaccgc gctctcccag acagtgctca tccgcgaact
      781 gatgctggcg accggggcgt tcgagatggt cgtagcggtc ggtctttcgc tgtggatgct
      841 cttcacaggt gtcggtgccg tcgcttccgg tatgttcccg gtccttcgtc gcccgttcgt
      901 gccgccggtg ctcgtcgctt tggtggcgat cgtcgccgtg gggcaacttt tccttgtgca
      961 tccgttggcg gcgctattcg gaatggtcgg cggcatggcg ctctcggtcc ccgcgatggt
     1021 cgtcgtgacg gcgatcgtac tcgcgcccgg ctgtcttgtc ggcggacttg cctttcccgc
     1081 ctgcgtcgcg gcggtgcgcg gcggcgcgga gcggacggga ctggtatacc tcattgaatc
     1141 gggcggtatg gcgctcggcg cggcactctt ctactttttt atggagttct tttcaccggt
     1201 acgtcacccg atcttcttcg cccactatca ggaacgctat cggcccgagg agcttatgct
     1261 ttcgcgcgac ggacgctccg gccgactcga tgtcacgagg cgcggcgacc ggatcgccta
     1321 tttctggaac gggcaggcgg ccggtgtcgt gggtccgaac aggcccgccg aggcctttgc
     1381 cggtctcgcg ctcacccagc ggcccgaccc gcggcgcatc gccgtcatcg gcgggcttct
     1441 tgcgggtgca gccgccgagg tggcgcgcaa aagccccgaa gcggtcgtca ccgtcatcga
     1501 gtccgagccg atgttcgcgg aggtcttcgc cgacgggacc gtcggccgca atggcgcgct
     1561 ccgtttcggc gatcccggcg cggtcctgcg ggaattgccg ccgcaggacc tcatcatcat
     1621 cgacctgccc gacccttcat cggtgtccct ttctcgtttc ttttcacggg agttcttttc
     1681 tttccttcag gaggataacg gtcccgcgat ggtgttggtc gtcggcctgt cgggcgggca
     1741 gggcatgctg acgcccgaaa cggcgtcgct caaccgatcg gtgcaattgg cactccgcgc
     1801 cgtgttccgg aaagtgcttc tcctcccggc atcgcgtcat ctatgggtcg cagccgaaaa
     1861 cgatctgccg accgccgatc cggtgttcat cgaagagcgg atggagcgga gcggacttgc
     1921 cggtgactgg ttcaacatgg cgctcgtgcg ggatgtgctt gagccgatgc accgggaact
     1981 gaccggcgcg gcgatagaaa gggcggatgc ccgcgagaac cgcctgctcc ggtcgtccgc
     2041 tcttttcgag accctgcgac acaccgcgcg gcggcttgac cggccgcttt cctccctgtt
     2101 ctcctccctg ccctcgcgcc cgttctctta tctgggggtg acgctcctct tcgccgcggc
     2161 ggtggctatc tttctctctc gcctgacccg gcggcgtctt gcgccggccc ggagcgccgc
     2221 gatattcgcc gtcagcggcg gtgccttcac cctgcaactg gccttgatgg cgctcctgca
     2281 acttcatgtg ggacagatat atcacctgat agccatcttc accgtttcgt tcacggtagg
     2341 gcttaccgca ggactctggg tatcgcggcg ggtggctctg ccgctgaacc tttccaccgg
     2401 ggcgctcgct cttgtgtcgc ttgcgacggc atggggcgcc gactccggga ccggcgcggc
     2461 ttttcccgtt gcgatgaatc tccttgccgg tctgacggcg gggctctcgt tcggcacgct
     2521 c
//
LOCUS       DAOQUE010000418         2519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_50172_length_2519_cov_3.902192, whole genome shotgun sequence.
ACCESSION   DAOQUE010000418 DAOQUE010000000
VERSION     DAOQUE010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50172_length_2519_cov_3.902192"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..810)
                     /locus_tag="PKH10_11250"
     CDS             complement(88..810)
                     /locus_tag="PKH10_11250"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT28738.1"
                     /translation="MRYPHAPIALLLFFCVSCDPGETTTILDQEMPDGDALADCPADM
                     APVGGVCVDKYEASRGDATADNAGDDETVARSVAGVLPWMVNPMTTTELAKFKSACVA
                     AGKRLCSVGEWTAACEGPEKNAYHFGNTFDRETCNCVDTFCDDFCAEHNIAVCNTDAN
                     CGYEYECFHVMPTGSFPDCTAGDGFFDVNGNVWEVVDNGSGGFLIKGGAFNCASAADR
                     LKCSFSAGWTELYAGFRCCKGR"
     gene            complement(822..1823)
                     /locus_tag="PKH10_11255"
     CDS             complement(822..1823)
                     /locus_tag="PKH10_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28739.1"
                     /translation="MQGLIALVLFLSPFLLSAEEEGPEDDITAISFSGSVGLGGGYES
                     DVAGSQGLVGDRFGAGDIIFDLSGEITLFEETAIGASFLHDVMPAYDGRYTRMAIGLT
                     ADWYRELGPVEIDLGAAGELSLIDRFDPEPYVGSFDGYAELVIPHGETLEWLVTLTGT
                     YQHGLRDDVRYLRGPDIFLQGAMRWHFDEDNGLVAGGYRAGVSLREEAGLYDLAGLAE
                     SGFTALAACNEHFEQALFIKASYDLDPLSFIGRFSAIHRYAFQKDAWATLSERKEKRR
                     IELVIVPSLEARVSFSDSSDISMQYDLEYTASTIGKRDYYDMNGLRHTVRILATQYF"
     gene            complement(1811..2113)
                     /locus_tag="PKH10_11260"
     CDS             complement(1811..2113)
                     /locus_tag="PKH10_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28740.1"
                     /translation="MEYHAAGVIQGAFERMRSCVAVLPVFFVLVTIVFPLRAGDTLPD
                     ADAVKPPSPAADEVVLDPVPTVDKDKEEKRREERKKERKEERKKKQQKSGKGKCKV"
     gene            2221..>2519
                     /locus_tag="PKH10_11265"
     CDS             2221..>2519
                     /locus_tag="PKH10_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28741.1"
                     /translation="MHMFRNAAFIAALLCGGSLLAGNQIVVITDGAGGCTYGGIKVQI
                     FDDNGTPGDPGDDSQIGIDYYVCNGAPGAKGDQGDPGVKGDKGDQGDPGLKGDKG"
BASE COUNT          521 a          801 c          712 g          485 t
ORIGIN      
        1 cgagtttcct gaccatgtga acactcccgt tatatagacc actcccctac catagcgaag
       61 cgaaagaatt tcacaaggaa aagccggtca gcgtcccttg caacaccgga acccggcata
      121 cagttcggtc cacccggccg agaacgagca tttcagccgg tcggcggcgc tcgcacagtt
      181 gaaggccccg ccctttatca gaaaaccccc cgaaccgttg tcgaccacct cccaaacatt
      241 gccgttgaca tcgaagaacc cgtcccccgc cgtacaatcg gggaacgatc cggtcggcat
      301 gacatggaaa cactcgtact catagccgca gttcgcgtcg gtgttgcata cggcgatatt
      361 gtgttcggcg cagaaatcgt cgcagaaggt gtccacgcag ttacaggtct cacggtcgaa
      421 ggtgttcccg aaatgatacg cattcttttc gggaccctcg caggccgccg tccattcgcc
      481 gaccgagcag aggcgcttcc ccgccgccac gcaagccgac ttgaacttcg ccagttccgt
      541 cgtcgtcatc gggttgacca tccacggcag gaccccggcg accgagcggg ccaccgtttc
      601 gtcgtccccc gcgttgtcag ccgtcgcatc acctctcgac gcctcgtact tatcgacgca
      661 gacaccgccg accggcgcca tgtcggcagg acaatcagcg agcgcatcgc cgtcaggcat
      721 ctcctgatcg agtatcgtcg tcgtctcgcc cggatcgcag gagacacaga agaagagaag
      781 cagggctatc ggggcatggg gatatctcat gacacgctcc atcaaaagta ttgggtcgcc
      841 agtatgcgga ccgtatgtcg cagcccgttc atgtcgtagt aatccctttt cccgatggta
      901 ctcgccgtgt attcgaggtc atactgcatc gagatgtctg aagagtcgga aaaagatacg
      961 cgtgcctcca gcgagggcac gatcaccagt tcgatgcgcc gcttctcctt ccgctcagag
     1021 agggtcgccc atgcatcctt ctgaaaggcg tagcggtgta tcgccgagaa ccgaccgatg
     1081 aacgagagag gatcgaggtc gtacgacgcc ttgatgaaca gggcctgctc gaaatgttcg
     1141 ttgcaggccg caagcgcggt gaagcccgat tcggcgagac ccgcgaggtc gtagagcccc
     1201 gcctcctcgc gcagggaaac gccggcccgg tatccaccgg cgacaagacc gttgtcctcg
     1261 tcgaaatgcc agcgcatcgc cccctgcagg aagatgtcgg ggccgcgcag gtatcgcaca
     1321 tcgtcgcgca ggccgtgctg gtaggtgccg gtgagcgtca ccaaccactc cagcgtctct
     1381 ccgtgcggta tcaccagttc ggcgtaaccg tcgaacgagc cgacataggg ctccggatcg
     1441 aaacggtcga tgagcgagag ttcccccgcg gcgccgaggt ctatctcgac cgggccgagt
     1501 tcacgatacc agtcggcggt cagtcctatc gccatacggg tgtagcgtcc gtcataggcc
     1561 ggcatgacat cgtgcaggaa tgaggcaccg atggcggtct cctcgaacag ggttatctct
     1621 cccgacagat cgaagatgat atctcccgcc ccgaagcgat cgccgacaag cccctgcgac
     1681 ccggccacat cgctttcgta gccgccgccc agccccaccg agccgctgaa cgagatcgcc
     1741 gtaatatcat cctccggccc ctcctcttcg gccgacagga ggaaagggga caggaacagg
     1801 acgagggcta tcagaccttg cattttccct tccccgattt ctgctgtttc ttcttccgtt
     1861 cctccttgcg ctcctttttc cgttcctcgc gccgcttttc ctccttgtcc ttgtcaacgg
     1921 tcgggacagg atcgagcacc acctcatcgg cggcggggga cggcggcttc accgcatcgg
     1981 cgtcgggaag cgtatcgccg gcacgcaagg ggaaaacgat cgtgacaagc acgaaaaaaa
     2041 cggggagaac ggccacacaa gaacgcatcc gctcgaacgc tccctgaata actccggcgg
     2101 catggtactc cataccattc ccccttgcaa gtgttttctc ccggaggttg acttgccccc
     2161 tgttctttgt ataatcactt cgagttccga gtgacgttcg ttcttatcat ggaggatatc
     2221 atgcacatgt tcaggaatgc ggcttttatc gcggcgcttc tttgcggcgg ctcgttgctc
     2281 gccggtaacc agatcgttgt tataaccgac ggggccggag gttgcaccta cggtggcata
     2341 aaagttcaga tattcgacga taacggtacc ccgggcgatc ccggcgatga ttcgcagatc
     2401 gggatagatt attatgtgtg caacggcgcc cccggcgcta agggcgacca aggtgacccc
     2461 ggcgtcaagg gagacaaggg cgaccagggc gaccccggcc tcaagggaga caagggcga
//
LOCUS       DAOQUE010000419         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_50210_length_2518_cov_2.714576, whole genome shotgun sequence.
ACCESSION   DAOQUE010000419 DAOQUE010000000
VERSION     DAOQUE010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50210_length_2518_cov_2.714576"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..>2518)
                     /gene="infB"
                     /locus_tag="PKH10_11270"
     CDS             complement(126..>2518)
                     /gene="infB"
                     /locus_tag="PKH10_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNT28742.1"
                     /translation="PEPEQPKAPVAIIRRERSYIPEEERAKLAEQQAQEPPATPAAPA
                     APTTAPQSEETPVTPPAEPAPGDETRPAEPAISPLDEMLLKPSFRIEAFATDRPITPA
                     LRPLGDQPQPEGEEDKPADHRHRKRKVVISETEDDRLPAQKKPVAARFFTKKKGKGDE
                     QVKIQAKIRGVDVSLFKSEEEEAEAKRRAAGKRKVIPRQRPVPMPKPNPTKAIKKIIR
                     IENAVVVSELARRMGIKAADIIRKLMMLGETLGINDRIPFETAGIVAGEFGFQVENVA
                     MDENSFLDTHENAPETLVPRPPVVTVMGHVDHGKTKLLDAIRNTNVVDREAGGITQHI
                     GAYTVELDKGRITFLDTPGHEAFTAMRSRGAQATDVVILIVAADDGVMPQTIEAINHA
                     KAANVPIIVAINKIDKPEANPERVRQELLKYEIVPEEWGGNNLFIEISAKQQINIDKL
                     LEAILLQVEMMELKANPDRPALGVVIESRMDPGRGPVATALVKEGTLRIGDLCATATA
                     LGYVRSMTDWRGTRVQEATPSTAVEITGLDSVPPAGEMIHVFTDEKKARGFVELRKNK
                     IKEQKYEKEERVSLDNLFAKIEKDDVKDFNLVVKGDVDGSVEAILGSLRKIEHEKLKI
                     RVIHSGVGGITENDVLLASASKAIVFGFNVRIDAKAKQTAVAEQVDIRIFSIIYDLID
                     AIKAAMSGKLAPIMHEEHVGTAEIRQVFRVSKVGNIAGCMVTDGRIVRKSRVKLIRDN
                     VVIYEGQLSSLKHFKDDAKEIKAGFECGLSIENYNDLKEGDVVECYVDTEVADEVL"
BASE COUNT          367 a          779 c          784 g          588 t
ORIGIN      
        1 ctcacccgga cctcctgtat caccatgttg ccgagcgggg acccgccgta ttcgcggatc
       61 atgatgaccg acagactctt gaatatcgcc tgagcgacgc gttgttgacg gacggacccg
      121 gccacctaga gtacctcgtc ggcgacctcg gtgtccacat aacactcgac gacatcgccc
      181 tccttcaggt cgttgtaatt ctctatcgag agcccgcact cgaagcccgc ctttatctcc
      241 ttggcgtcat ccttgaagtg cttgagcgaa ctcaactggc cctcgtagat gaccacattg
      301 tcgcggatga gtttgacgcg gctcttgcgg acgatgcgac cgtcggtcac catgcagccg
      361 gcgatgttgc cgaccttgga aacgcggaag acctgccgta tctcggcggt gccgacatgt
      421 tcctcgtgca tgatgggcgc gagtttgccg ctcatggccg cctttatcgc gtcgatgagg
      481 tcgtagatga tggagaagat gcggatatcc acctgttcgg cgaccgccgt ctgtttcgcc
      541 ttggcgtcta tgcgaacatt gaagccgaac acgatggcct tcgacgccga cgccagcagg
      601 acatcgttct cggtgatgcc gccgacgccg ctgtgaatga cccgtatctt gagtttctca
      661 tgctctatct tgcggagcga tccgagtatc gcctcgaccg agccgtcgac atcacctttc
      721 acgacgaggt tgaagtcctt gacatcgtcc ttttctatct tggcgaacag gttgtcgagc
      781 gacacgcgct cctctttctc gtatttctgc tccttgatct tgttcttccg gagttccacg
      841 aagccccgcg ccttcttctc gtcggtgaag acatggatca tctcgcccgc cggcggcacc
      901 gaatcgagcc cggtgatctc gaccgcggtc gaaggggtcg cttcctgcac gcgggtaccg
      961 cgccagtcgg tcatggagcg gacatagccg agcgcggtgg cggtggcgca caggtcgccg
     1021 atccggagcg tgccctcctt tacgagcgcc gtggcgaccg gcccgcggcc cgggtccatg
     1081 cgcgactcaa tgacgacgcc gagcgccggc ctgtcgggat tggccttgag ttccatcatc
     1141 tcgacctgca ggaggatcgc ctcgaggagt ttgtcgatgt ttatctgttg tttggccgat
     1201 atctcgatga acaggttgtt gccgccccac tcttcgggga ctatctcgta tttgagcagt
     1261 tcctgacgga cccgttcggg attcgcttcg ggcttgtcta tcttgtttat cgcgacgatg
     1321 atcgggacat tcgccgcctt cgcgtggttg atcgcctcga tggtctgcgg catcacgccg
     1381 tcgtcggccg ccacgatgag gatgacgaca tcggtcgcct gcgcgccgcg cgagcgcatg
     1441 gccgtgaagg cctcgtggcc cggggtgtcg aggaaagtga tgcggccctt atcgagttcg
     1501 accgtgtagg cgccgatgtg ctgggtgatg cctcccgcct cgcgatccac cacattggtg
     1561 ttgcgtatgg cgtcaagcag tttggtcttg ccgtggtcga catggcccat gacggtgacg
     1621 accggcggtc gggggaccag cgtctcgggg gcgttctcgt gcgtatcgag gaaactgttt
     1681 tcgtccatcg ccacattctc gacctgaaaa ccgaactcgc cggcaacgat gccggccgtt
     1741 tcgaacggga tacggtcatt gatgccgagc gtctcgccga gcatcatgag tttgcggatg
     1801 atatcggccg ccttgatacc catgcgtcgc gccagttcgg aaacgacgac ggcgttctcg
     1861 atgcggatga tcttctttat cgccttggtc gggttcggtt tcggcatggg gaccgggcgc
     1921 tggcgcggga tgaccttccg tttaccggcg gcccgccgtt tcgcctcggc ctcctcctcc
     1981 tcgctcttga acagggagac atcaacgccg cgtatcttgg cctgtatctt gacctgttcg
     2041 tcgcccttcc ccttcttttt tgtgaagaaa cgggcggcga ccggtttttt ctgcgcgggc
     2101 agacggtcat cctcggtctc cgatatcacc accttccgtt tccggtgacg gtgatcggcc
     2161 ggtttatcct cctcgccttc cggttgcggc tggtcgccca gcgggcgcag ggcgggggtg
     2221 atcggccggt cggtcgcgaa tgcttcgatg cggaacgatg gcttcaggag catctcgtcg
     2281 agcggcgata tcgccggttc cgccggtcgg gtctcgtctc ccggcgccgg ctcggccggt
     2341 ggggtcacgg gcgtctcttc cgattgcggc gcggtcgtcg gcgcggcggg tgcggcaggc
     2401 gtggccggag gttcctgcgc ctgttgctcc gccagttttg cgcgttcttc ctcggggatg
     2461 taactgcgct cccgccggat gatggcgacg ggcgccttcg gttgctccgg ttccggtt
//
LOCUS       DAOQUE010000420         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_50350_length_2513_cov_3.561025, whole genome shotgun sequence.
ACCESSION   DAOQUE010000420 DAOQUE010000000
VERSION     DAOQUE010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50350_length_2513_cov_3.561025"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PKH10_11275"
     CDS             <1..982
                     /locus_tag="PKH10_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HNT28743.1"
                     /translation="DVAKRKAFFNDLVFSPGGMEVHAHGTLAERRFDLSVDMRDLPLN
                     LLATLVKNRLFDGFLMEGTIGVSLAAAGEILPGDVTLEAISISGSLNGVKQLSDRFDR
                     YRSGFDYFFTDDTGAAFRVEVKNNKFTYAPLALLPPYVPRAVVLSEDAGFFLHHGIDF
                     AEMDAAFKDNLTRHELRGGSTITQQLVKNLFLTRNKTILRKFRELLLAVELDATLSKE
                     RILEIYLNVIEWGPGIFGIGQAAHHYFGKLPEELLPHEAAWLASIIPNPKRFYHEYRS
                     GSVSEARQGRIQRLLDLLFESGHISGDLYIACYTAPLIFRSETEGTGGGW"
     gene            979..1674
                     /locus_tag="PKH10_11280"
     CDS             979..1674
                     /locus_tag="PKH10_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNT28744.1"
                     /translation="MKVSPVVSASCLSCGVMIPAGDMFCGDCVPYALALTRPCPRCGG
                     FIARSNEDDGGCRFCRGRDFSFDAAASPFIYAGAIAKAVTTMKYGPIYRMAVAMGRWL
                     GDRVPDEMRIADRVVFPPMNRMALFLRGFNQAAVIADEVARRAGMFLDPHTLKKVKRT
                     KQQAELSLDERKRNLAGAFALTRPVTGKRFLLIDDVATTLTTANEIARLLKMNGADKV
                     FVLTFARKSLHFD"
     gene            1742..>2513
                     /locus_tag="PKH10_11285"
     CDS             1742..>2513
                     /locus_tag="PKH10_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium symporter"
                     /protein_id="HNT28745.1"
                     /translation="MAGQREHWGSKLGVILAVAGSAVGLGNFLRYPAKMALNGGGAFM
                     IPYLVAFLLLGIPLMWMEWTLGRMGGQRGFGTATSIFASVTRNSMVARILGAIGILGP
                     FIILVYYTFIESWTLAYAYFAFTGELTTMGDQAGMKAFLSGYQGLVANEHFSGIGIAY
                     IFFMITFSLNFFFIYRGIQGGIEKLSRIGMPILLILAVLLVIRTLSLGTPDPAQPELS
                     ITNALNFIYTPDLASLGNAKVWLEAAGQIFFTLSIGMGV"
BASE COUNT          450 a          786 c          734 g          543 t
ORIGIN      
        1 cgatgtggcc aagcggaagg cgtttttcaa cgatctggtc ttctcgcccg gcgggatgga
       61 agttcacgcg cacggtacgc tggcagagcg ccggttcgac ctttcggtgg acatgaggga
      121 tcttccgctc aatctgctcg cgacgctcgt gaagaatcgc ctgttcgacg ggtttctgat
      181 ggaggggacg atcggcgtgt cgcttgcggc ggcgggcgag atccttcccg gcgatgtcac
      241 gctcgaagcc atatccatca gcggctcgct gaatggggtg aagcagttgt ccgaccggtt
      301 cgatcgctac cggagcgggt tcgattattt cttcactgac gataccggcg ccgcgttccg
      361 ggtcgaggtt aagaacaaca agttcaccta cgcgccgctc gccctccttc ctccctatgt
      421 gccgcgcgcg gtggtgctgt cggaggacgc cggattcttc ctacaccacg gcatcgattt
      481 cgccgaaatg gacgccgcct tcaaggataa tctcacgcgc catgaactgc gcggcggctc
      541 gaccataacc caacaactcg tcaagaatct cttcctgacc cgcaacaaaa cgatcctgcg
      601 gaaattccgg gaacttcttc tcgccgtgga actggacgcc accctctcca aggagcggat
      661 actcgagata tatctcaatg ttatcgagtg ggggcccggc atattcggga tcggtcaggc
      721 ggcccatcac tacttcggca aactgcccga ggaactcctg ccgcacgagg cggcatggct
      781 cgcctccatc atccccaacc cgaagcgctt ctaccacgag taccgtagcg gttctgtatc
      841 cgaagcgcgg caagggcgta tccagcgcct gctcgacctg ctgttcgagt cgggccatat
      901 ctccggcgat ctctatatcg cctgttacac cgcgccgctc atcttccgta gcgagaccga
      961 agggacggga ggcggctggt gaaggtttcc cccgtcgtgt cggcctcgtg cctttcctgc
     1021 ggcgtcatga tacccgccgg cgatatgttc tgtggtgact gcgttcccta cgcccttgcc
     1081 ctgacgcgtc cttgtccccg gtgcggtggc tttatcgcgc gatcgaacga agatgacggc
     1141 gggtgccggt tctgccgcgg tcgggatttc tccttcgatg cggcggcgag tcccttcatc
     1201 tacgccgggg cgatcgccaa ggcggtcacg acgatgaagt atggcccgat ctaccgcatg
     1261 gcggtggcga tgggacggtg gcttggtgac cgagtgcccg acgagatgcg cattgccgac
     1321 cgcgtggtgt tcccgccgat gaaccgtatg gcgctctttt tgcgcgggtt caatcaagcg
     1381 gccgtcatcg ccgacgaagt ggctcgacgg gccggaatgt tcctcgatcc ccacaccctc
     1441 aagaaggtga agcggaccaa acagcaggcc gaactgtcgc tcgacgaacg gaaacggaac
     1501 cttgcgggcg ccttcgccct cacccggccg gtgaccggca agcggttcct cctcatcgac
     1561 gatgtcgcga cgaccctcac caccgccaat gagatagcga ggctactcaa gatgaacggc
     1621 gcagacaagg tcttcgtgct gaccttcgcc cgcaaatcgc ttcacttcga ctgaatttgt
     1681 tgttatttcc cctcgtttct gctagggtgg ggccgggtaa gccattcatc gggggctttt
     1741 aatggctgga cagagagaac actggggctc gaaactcggc gtcattctcg cggtcgcggg
     1801 gagcgcggtg gggctgggca attttctgcg ctatccggcg aagatggccc tgaacggcgg
     1861 cggcgccttc atgataccct atcttgtcgc gttcctcctg ctcggtatcc cactcatgtg
     1921 gatggaatgg acactcggcc ggatgggcgg ccagcgcggc ttcggcacgg cgacctcgat
     1981 cttcgcctcg gtcacccgca atagcatggt cgcccgcatc ctcggggcta taggcatcct
     2041 tggtcctttc atcatcctcg tttattacac cttcatcgaa tcgtggacgc tcgcctatgc
     2101 ctacttcgcc ttcaccggag aactgacgac gatgggcgat caggcaggga tgaaggcgtt
     2161 cctgtcgggc tatcagggac tggtcgccaa tgaacatttc tccggcatcg gcatcgccta
     2221 tatcttcttc atgatcacct tctctcttaa cttcttcttc atctaccgcg gcatacaggg
     2281 cggtatcgag aaactttccc gcatcgggat gccgatactg ctgatactcg cggtgctgct
     2341 ggtcatccgg acgctgtcgc tcggcacccc cgatccggca caaccggaac tatcgataac
     2401 aaacgcgctc aatttcatct acacccccga cctggctagt ctcgggaacg ccaaggtctg
     2461 gctggaggcg gcggggcaga tattcttcac cctatccatc ggcatggggg tca
//
LOCUS       DAOQUE010000421         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_50413_length_2511_cov_3.745928, whole genome shotgun sequence.
ACCESSION   DAOQUE010000421 DAOQUE010000000
VERSION     DAOQUE010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50413_length_2511_cov_3.745928"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PKH10_11290"
     CDS             <1..480
                     /locus_tag="PKH10_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HNT28746.1"
                     /translation="TVIPPTASVSGPAIIGKQVEIRHGAFIRGSAIIGNGCVIGNSCE
                     IKNAFIFDAVQIPHFNYVGDSVMGYRSHIGAGVILSNVKSVPGNVTVKCKDCFIDTGL
                     RKFSAIIGDHTEAGCNTVFNPGTIVGKECVIYPLTSVRGVIPARTIVKDDGKMMQKR"
     gene            503..2107
                     /locus_tag="PKH10_11295"
     CDS             503..2107
                     /locus_tag="PKH10_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28747.1"
                     /translation="MFRKPMLLFLPLMLLVACDSGKPSTDVDIQWDLDPVIDGEGTDT
                     DEKPLPDVDEEKDEKDISDIKDDEQPEDPDDAIASDDDALLTDGPVDEDELTDDGDLL
                     LDLCFEKACGAHGDCSVPDHACAPYLSTEAYACGPDVEDFGYRGEGLCVEVNCAIDAD
                     CREDLGYLCVPFGGPLPEFNDASSACLRSLSGSSCSEEGKKVCQFGKDIAITCADGVW
                     EPYFCEEGWFCEAGECILEVLDGDGELPDADTLPPVEICDGVDNNGNTLTDEGCDTDG
                     DGWCDITMVVYGTPAVCPNGLLDCDDEDPDIYPGSTIHQEGIDYDCDNRKEYQAIMTL
                     SVDDQLVELCANGTQFSSAEFGIYYGNWAYADTYGGPKLVLESGTNVVGVHGKDTGLA
                     ISSFVAVILVNGETIITDGVLPPGDGNEYTPLDPEWTDAQWRYFPEAVPTPNADWCDK
                     WFDDSDWGPAILAGKAGTDPSVYGNLGTDPWWGGACGLTQYERCPTAFESYYTDATAG
                     NEPKWIWDYNPTQLADAWLRIRIVLP"
     gene            2119..>2511
                     /locus_tag="PKH10_11300"
     CDS             2119..>2511
                     /locus_tag="PKH10_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28748.1"
                     /translation="MKNFSFIAPLLLLFVALSCDSVKPAAQTDQDNDLNDDDIASENS
                     LPDMDSDQSDPTDQADPSDPSDDDALVSDDLLPDDDPLLPGDHDGDGIPDSVEKPGGI
                     AVDTDNDGIPDFKDDDSDGDGIPDAVECP"
BASE COUNT          524 a          765 c          772 g          450 t
ORIGIN      
        1 accgttatcc cgccgaccgc gtcggtaagt ggtcccgcga tcatcgggaa gcaggtcgag
       61 atacggcacg gcgccttcat ccgcggtagc gccatcatcg gcaacggctg cgtcatcggc
      121 aattcgtgcg agataaagaa cgcgttcatc ttcgacgcgg tgcagatccc tcatttcaac
      181 tatgtcggcg actcggtgat ggggtatagg tcacacatcg gcgcgggggt catcctctcc
      241 aatgtcaaat cggtgccggg caatgtcacg gtcaagtgca aggactgctt catcgatacc
      301 ggcctgcgga agttctcggc gatcatcggc gaccacaccg aggcgggctg caacaccgtt
      361 tttaatcccg gcaccatcgt cgggaaggag tgcgtcatct acccgttgac gagcgtccga
      421 ggggtcatac cagcccgcac catcgtcaag gatgacggca agatgatgca gaaacgctga
      481 ggatcttaag gggagggggg ccatgttccg aaagccgatg ctcctgtttc ttccgctgat
      541 gctccttgtc gcctgtgaca gcggcaaacc atctacggat gtcgatatcc agtgggacct
      601 cgatccggta atcgacggag aaggtaccga caccgatgaa aaaccacttc ccgatgtgga
      661 tgaggaaaag gacgaaaagg acataagcga cataaaggac gatgaacagc cggaagaccc
      721 tgacgacgcg atcgcatcag acgatgacgc cctgctgacc gacggaccgg tcgatgagga
      781 tgaactcacc gacgacggcg acctgcttct ggacctctgc ttcgaaaagg cctgtggtgc
      841 gcacggcgac tgcagtgtcc ccgatcatgc ctgcgccccg tatctttcga ccgaagcgta
      901 cgcctgcggt cccgatgtcg aggacttcgg ctaccgcggc gagggcctgt gcgtcgaggt
      961 gaactgcgcg atcgacgccg actgtcgtga ggacctcggc tacctctgtg tccccttcgg
     1021 tggcccgctc cccgaattca acgacgcctc gtcggcctgt ctccggtcgc tttcggggtc
     1081 ttcctgcagc gaagagggta aaaaggtctg ccagttcggg aaagatatag cgataacctg
     1141 cgccgatggc gtatgggaac cctatttctg cgaggagggg tggttctgcg aggcgggtga
     1201 gtgtatcctc gaggtgctcg acggcgacgg ggaactgccc gatgccgata ccctgccgcc
     1261 ggtggagata tgcgacggtg tcgacaataa cgggaacacc ctgacggatg aaggatgcga
     1321 caccgacggc gacggctggt gcgacatcac gatggtcgtc tacgggacgc ccgccgtctg
     1381 tcccaacggc ctgcttgact gtgacgacga ggaccccgac atctatcccg gttcgacgat
     1441 tcatcaggag gggatcgatt acgactgcga caaccgcaag gagtatcagg cgatcatgac
     1501 cctgtcggtc gatgaccagt tggtcgaact gtgcgcgaac ggcacgcagt tctcttcggc
     1561 ggaattcggc atctactacg ggaactgggc ctacgccgac acctacggcg gccccaaact
     1621 ggtgctcgaa tcgggcacca atgtcgtcgg cgtgcacgga aaggacacgg ggctcgcgat
     1681 ctcatccttc gtagcggtca ttttggtgaa cggcgagacc atcatcaccg acggtgtcct
     1741 gccgccgggc gacgggaacg agtacacgcc gctcgacccc gaatggaccg acgcccagtg
     1801 gcgctacttc cccgaagcgg tcccgacccc gaacgccgac tggtgcgaca aatggttcga
     1861 cgacagcgac tggggtcccg cgatactggc cggaaaagcg gggaccgacc catcggtcta
     1921 tggcaacctc gggaccgacc cgtggtgggg aggggcctgt ggcctgacgc agtacgaacg
     1981 gtgcccgacc gcgttcgagt cgtactatac cgacgccacg gcgggcaacg agcccaaatg
     2041 gatatgggac tacaatccga cgcaacttgc cgatgcatgg ctgcggatac ggatcgtgtt
     2101 gccgtgaggg agggaagcat gaagaacttt tcgttcatcg cgccgctcct gctccttttc
     2161 gtcgcccttt cgtgcgacag tgtcaaaccg gccgcccaga ccgaccagga taacgacctc
     2221 aatgacgacg acatcgcgtc ggagaattcc ctgcccgaca tggacagcga ccagtccgat
     2281 ccgaccgatc aggccgaccc ctccgacccg tccgacgatg atgctcttgt gtccgacgac
     2341 cttcttcccg acgacgatcc tctgcttccc ggcgaccacg atggcgacgg cattcccgac
     2401 agtgtcgaga agccgggcgg catagcggtc gataccgata acgacggtat ccccgacttc
     2461 aaggacgacg acagcgacgg cgacggcata cccgatgcgg tagagtgtcc c
//
LOCUS       DAOQUE010000422         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_50718_length_2501_cov_3.624285, whole genome shotgun sequence.
ACCESSION   DAOQUE010000422 DAOQUE010000000
VERSION     DAOQUE010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50718_length_2501_cov_3.624285"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /gene="cheB"
                     /locus_tag="PKH10_11305"
     CDS             complement(<1..920)
                     /gene="cheB"
                     /locus_tag="PKH10_11305"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNT28749.1"
                     /translation="MMEVRNEKIKVLVIDDSATARRAIEDIVASAPDMQVIGSAADAY
                     EARECMRRAMPDVITLDIEMPRMDGLTFLDKLMRAVPVPVIMISSLTQEHAAATMRAL
                     ELGAVDYIGKPNKGEGSDIESLSEEILEKIRAAAGVPRSWLERHRLRSRMTIAPEPPL
                     DPSEMGLVQSATRITDLRPLIAIGASTGGTVAIEQLLRRLSPHRMPPILIVQHIPPLF
                     SRSFAERLNSLFDFSVSEARHGHELKPGEVVVAEGGRHLIVERGPAGYTIISKDGPRV
                     NRHRPSIDVLFRSVASAARENAIGVILTGMG"
     gene            complement(922..1524)
                     /locus_tag="PKH10_11310"
     CDS             complement(922..1524)
                     /locus_tag="PKH10_11310"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNT28750.1"
                     /translation="MYRSFNRKLGKEIVIIQPGEFYVTDAPDEGIATVLGSCVAVCLL
                     DTGSTVRGMNHFMLPGDFRNEEIYQSRSGRYGMFAMESLINEMMKRGARRESLVAKIF
                     GGAHVLNFRRSDGNIPDANISFTQTYLAFEEIRLAASDIGGTTGRKVIFLGEGTGRVL
                     VKKLSPESTSQYIDEENRYKAKILRKKKEEMSGSITLFDK"
     gene            complement(1527..2360)
                     /locus_tag="PKH10_11315"
     CDS             complement(1527..2360)
                     /locus_tag="PKH10_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNT28751.1"
                     /translation="MLEIKKFVLTDEEFNKLRRLVFDTAGISLSDAKRELVVSRFSRR
                     LRALNLSTFGDYYHFVISPEGQSEIQNFINSITTNKTDFFRETHHFDFLRDQFVPACV
                     RSGRREVAVWSAGCSTGEEPYTIAMVLHRHLVDPHGIRVRVLATDIDTHVLDVARNGV
                     YEERQIRLIPEDMQHRYFLRGKGESTGLYKVKENLREMVTFRQINFVAEQYPVSSLFD
                     VIFCRNVVIYFNNETKLQVLSRLAGYLVRGGFFIIGHSETLFDMVPGLTYIKNTVYRK
                     D"
BASE COUNT          450 a          718 c          811 g          522 t
ORIGIN      
        1 cccatgccgg tcaggatgac gccgatggcg ttctcccgtg cggccgaggc gaccgaccgg
       61 aacaggacat cgatcgaggg acggtggcgg ttcacccgcg ggccgtcctt gctgatgatc
      121 gtatatcccg ccgggccacg ctccacgata aggtgccggc cgccctcggc gacgaccacc
      181 tcgcccggtt tcagttcatg accgtgtcgc gcctcgctga cggagaaatc gaacagcgag
      241 ttcagccgtt cggcgaagga tcgcgagaaa agaggcggga tatgctgaac gatgagtatg
      301 gggggcatgc ggtgcggcga gagacgccgc aggagttgtt cgatggcgac ggtgccgccg
      361 gtcgatgcgc cgatagcgat gagcggtcgc agatcggtga tgcgtgtcgc agactggacg
      421 agccccatct cactcgggtc gagcggcggt tcgggcgcta tggtcatgcg gctgcggagc
      481 cggtgccgct cgagccacga gcgagggacg cccgccgcgg cacgtatctt ttcgagtatc
      541 tcctccgaca gggactcgat gtccgatccc tcccccttgt tcggtttgcc gatgtagtcg
      601 acggcgccga gttccagcgc ccgcatggtc gccgccgcgt gttcctgcgt gagcgaactt
      661 atcatgatga ccggcaccgg aacagcccgc atgagtttgt cgagaaaggt aagcccgtcc
      721 atgcgcggca tctcgatgtc gagtgttatg acatcgggca tcgcgcggcg catgcattcg
      781 cgcgcctcgt aagcgtcggc agccgagccg atgacctgca tgtcgggggc cgaggcgacg
      841 atatcctcga tggcgcggcg ggcggtcgcc gagtcgtcta tcacgaggac ctttatcttt
      901 tcgttgcgta cttccatcat gtcatttgtc gaacagcgtg atgctgccgc tcatttcctc
      961 ttttttcttg cgcaatatct tcgccttgta gcggttctcc tcatcgatgt actgggaggt
     1021 gctttcgggc gagagtttct tcacaaggac gcgcccggtc ccttcgccga ggaagatgac
     1081 cttccgtccg gtggtgccgc cgatgtcgga ggcggcgagc cgtatctcct cgaaggcgag
     1141 ataggtctgg gtgaacgata tgttcgcgtc gggtatgttg ccgtcgctcc ggcggaaatt
     1201 gagcacatga gcgccgccga atatcttggc gacgaggctc tcgcgccggg cgccgcgctt
     1261 catcatctcg ttgatgagac tctccatcgc gaacatgccg taccggccgc tcctgctttg
     1321 gtagatctcc tcgttgcgga agtcgccggg aagcatgaaa tgattcatgc cgcggacggt
     1381 cgatccggtg tcgagcaggc agaccgcgac gcaggaaccg agcacggtgg cgataccctc
     1441 atcgggcgca tcggtgacat agaactcgcc gggctgtatg atgacgatct ctttcccgag
     1501 tttgcggttg aacgagcggt acacggtcaa tccttccggt aaacggtgtt ctttatgtag
     1561 gtcagtccgg ggaccatatc gaagagggtc tcgctgtgcc cgatgatgaa aaagccgccg
     1621 cgcaccagat agccggccag gcggctgagc acctgcagtt tggtctcatt gttgaaatag
     1681 atgacgacgt tccggcagaa gatgacatcg aacagcgagg agaccggata ctgttcggcg
     1741 acgaaattta tctggcggaa ggtgaccatc tcgcggaggt tctccttgac cttgtagagt
     1801 ccggtgctct cccccttgcc gcgcaggaaa taccggtgtt gcatgtcttc agggatgagc
     1861 cgtatctgcc gttcctcgta gacgccgttg cgggcgacat cgagcacatg ggtgtcgatg
     1921 tcggtcgcca gtaccctcac gcggatcccg tgcggatcga ccagatgacg gtgcaggacc
     1981 atggcgatgg tgtagggctc ctcgccggtg gagcagccgg ccgaccagac cgccacctcg
     2041 cggcgaccgc tccggacgca cgccgggacg aactggtcgc gcaggaaatc gaaatggtgg
     2101 gtctcgcgga agaagtcggt cttgttggtc gtgatggagt ttatgaagtt ctgtatctcg
     2161 ctctgaccct cgggcgatat gacaaaatgg taatagtcgc cgaaggtcga gagattgagc
     2221 gcccgcagtc gtcgggagaa gcgtgacacc acgagttccc gtttggcgtc ggataaactg
     2281 atgccggcgg tatcgaacac cagccgccgg agtttgttga actcctcgtc ggtgagaacg
     2341 aatttcttga tctcaagcat cgctatctcg acagcatccg ttcgatgtcc agtacgatga
     2401 ggagggcgcc ggtccgttgg tctttcccta tgaagcgcac atattcggcg gtggtacggc
     2461 ggccgaggag cggcttggcc tgcttgtcct ccggctccag c
//
LOCUS       DAOQUE010000423         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_50846_length_2497_cov_3.509419, whole genome shotgun sequence.
ACCESSION   DAOQUE010000423 DAOQUE010000000
VERSION     DAOQUE010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50846_length_2497_cov_3.509419"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="PKH10_11320"
     CDS             complement(<1..1510)
                     /locus_tag="PKH10_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT28752.1"
                     /translation="MSRLRRMLLVALLTLLTALSLWGETIIVADIEDRTGQLKPEVLE
                     ASRGLLEQAYRASGKHQVLPVSSLRKFVADQKKWKDCYGAECQIAAATALKADLVLGA
                     SIDFFAGIYTMTVSLVNAKTKQKNEAGAADFNGTAVGMKGAIEKLAPALTGKKMAAAP
                     AAVPAPATPAPAAPPPPSDMEKKYRGAAFQSAPKVETYAAPIAVKEPEIKKADNGGVS
                     FTSQLAAVKILFAKGKKEEKTCTAPCTVTNLEPGDYVARFEKDGFSPMEEAIRIEAGK
                     TIPYAIQLTSLIAEKAKLSEELIEAAKNGDIKEMRRLFVLGADVNYRNAGGYGPLLVA
                     ALSGQTEAATILIARGAKFSDVEIGTLLLMVVETNNLALFRLMLSQATDFNYRYDEGR
                     TILWLAVEKQRWSIFDQLVKSKKVDTGIKDNSGMTILMWTIENGMEKVADMLKNYGLK
                     LPPDLAARALRNSVAKENILKLRTLLPFVTNINPVYEDGLTPRLEVTQPVLTN"
     gene            complement(1624..1701)
                     /locus_tag="PKH10_11325"
     tRNA            complement(1624..1701)
                     /locus_tag="PKH10_11325"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1664..1666),aa:Pro,seq:cgg)
     gene            complement(1785..>2497)
                     /locus_tag="PKH10_11330"
     CDS             complement(1785..>2497)
                     /locus_tag="PKH10_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNT28753.1"
                     /translation="KVTQTSYKLYPVNVKKKVGKGQFELVGTKVKENPAVLELLKSFS
                     CEFSTQPVGETEMEFSGKKELLRFKEAELGNVITDIMRTRTGADIAFVNGGGIRQGLK
                     KGLVTEKDIYSVFPFNDTIVVGQVTGNELQAIADLFVAKKVGSGGFLHFSGLSLSANK
                     GKVIEILVNGQPLEKEKKYSIAMNSYLAGGGDGYTMLKDLRDKRFTGLSVPAVIVEYL
                     KAEKIISRPKTGRVTISK"
BASE COUNT          421 a          722 c          759 g          595 t
ORIGIN      
        1 cgttggtgag cacgggctgg gtcacctcca gacgcggggt gaggccatcc tcgtacacag
       61 gatttatgtt ggtgacaaag ggcagaaggg tgcggagttt caagatattc tccttggcga
      121 ccgagttccg gagggcgcgc gccgcgaggt cgggcgggag tttgagcccg tagttcttca
      181 gcatgtctgc gaccttctcc atcccgttct cgatggtcca catgaggatg gtcatgcccg
      241 agttgtcctt tatcccggtg tcgaccttct tggacttgac caattggtcg aatatggacc
      301 agcgttgttt ctccacggcg agccagagta tggtgcgtcc ctcgtcgtag cggtaattga
      361 aatcggtggc ctggctgagc atgagccgga acagggcgag gttgttggtc tcgaccacca
      421 tgagcagaag cgtaccgatc tcgacatcgc tgaacttcgc gccgcgggcg atgaggatag
      481 tggccgcctc ggtctgtccg ctgagcgccg cgacaaggag cgggccgtag ccgccggcgt
      541 tgcgatagtt gacatcggcg ccgagcacga acaggcggcg catctccttg atgtcgccgt
      601 tcttggccgc ctcgatgagt tcctcgctca gtttggcctt ttccgcgatg agcgaggtga
      661 gttgtatggc gtagggaatg gtcttgcccg cctcgatgcg gatggcctcc tccatagggg
      721 aaaagccgtc cttttcgaag cgcgcgacat agtcgcccgg ttcgaggttg gtgacggtgc
      781 agggggcggt gcaggtcttt tcctcctttt tgccttttgc gaatagtatc ttgacggcgg
      841 cgagttgcga ggtgaaggag acgccgccgt tatcggcctt tttgatctcc ggttccttga
      901 ccgctatcgg cgcggcgtag gtctcaacct tcggggccga ctggaacgcg gcgccccgat
      961 atttcttctc catgtcgctg ggcggtggcg gcgcggcggg tgccggcgtc gcgggtgccg
     1021 ggactgccgc cggcgcggcg gccatttttt ttccggtcag agccggggcc aacttctcaa
     1081 tggccccctt catgccgaca gccgtgccat tgaaatcggc cgcgcccgcc tcgttcttct
     1141 gtttggtctt ggcgttgacg agcgacacgg tcatcgtata gatgccggcg aagaaatcga
     1201 tcgatgcgcc gagcacgaga tcggctttca gcgccgttgc cgccgctatc tgacattcag
     1261 cgccgtagca gtccttccat ttcttctggt cggcgacgaa cttgcggagc gacgagaccg
     1321 gcagaacctg atgttttccc gatgcgcggt aggcctgctc cagcaggccg cgcgatgcct
     1381 cgagcacctc cggtttcaac tgtccggtcc ggtcctcgat gtcggccacg atgatcgtct
     1441 cgccccaaag cgatagtgcc gtcagcaagg tgaggagtgc gacaagcagc attctgcgca
     1501 agcggctcat gtgtaaacct ctcacaattg ttcctgtatc tttatcatgg cgcccatcat
     1561 aacacagacg accccgaaga caactatttc ttattcgttc gagcgatttt ttttgaaaaa
     1621 aaatggtcgg gatgactgga ttcgaaccag cggcccctgc gtcccgaacg cagtgctcta
     1681 gccaggctga gccacatccc gacctcggtg gtcgcacctt tttatacccg cctttgaccc
     1741 ttttgcaact tttttgtggg gccgcgaccg gtatgtcgcc gaggttattt ggaaatggtg
     1801 acgcggccgg tcttgggacg cgagatgatc ttctcggcct tgaggtattc gacgatgacg
     1861 gcggggaccg acagcccggt gaaccgtttg tcgcgcagat ctttgagcat cgtgtagccg
     1921 tcgccgccgc cggcgaggta actgttcatc gcgatggagt atttcttctc cttttcgagc
     1981 ggctggccgt tcacgagtat ctcgatcacc tttcccttat tcgccgacag ggaaagtccc
     2041 gagaaatgga ggaagccacc gctcccgacc ttcttggcca cgaacaggtc ggcgatcgcc
     2101 tgcagttcgt tgccggtcac ctgtcccacg acgatggtgt cgttaaaagg gaagacgctg
     2161 tagatgtcct tttcggtcac gagacccttc ttgagcccct gccggatgcc gccgccgttg
     2221 acgaacgcga tgtcggcgcc ggtgcgggtc cgcatgatat cggtgatgac attgcccagt
     2281 tcggcctcct tgaagcggag cagttccttt ttgccgctga actccatctc ggtctctccg
     2341 accggctggg tggagaattc gcaactgaaa cttttcagca gttcgagaac cgccgggttc
     2401 tccttcacct tggtgccgac cagttcgaac tggcctttgc cgaccttctt tttgacattg
     2461 accgggtaga gtttgtaact ggtctgcgtc accttgc
//
LOCUS       DAOQUE010000424         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_51418_length_2480_cov_3.105155, whole genome shotgun sequence.
ACCESSION   DAOQUE010000424 DAOQUE010000000
VERSION     DAOQUE010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51418_length_2480_cov_3.105155"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKH10_11335"
     CDS             <1..212
                     /locus_tag="PKH10_11335"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT28754.1"
                     /translation="DAGNNDGAIGLFLQAIELDPADGDAYYGLGYAYHAKGQKDKARN
                     YYQRALQSRLKEADKADIRSILEKM"
     gene            264..>2480
                     /locus_tag="PKH10_11340"
     CDS             264..>2480
                     /locus_tag="PKH10_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNT28755.1"
                     /translation="MERRFFWAMSVLLLLVSCGGSKSVTGTDPLAVTDDPTVPDIDFT
                     PPDKYDDHAAEWKKVESAEKEGKPRTALEEIAKILDAARAEHNVPQIVKALIQRAKFE
                     QRIGEKQPADQIAEIEKEIAAARFPEKNLLHSVAADLYESYFQMNRWRFYERTAGATE
                     GEDIASWSLPKLMATITAHHHAALEHGATLREIKIELLDEVLTKGTRPRTIRPTLYDL
                     IAHRAADFFMNDENSLAEGAAPFEVRDLGYLFPAERFAILPITTTDRNALAWHALTIL
                     QDLTRAHLRDTDPAALVDIELKRLAFVRSIHTADNRDDIYLKTMELLEQQYGAHAASA
                     SIGYERARFSYEKGLAWREGMPDAERLGLKEAVTKCGAVTLKWPDSEGAALCRALSSD
                     ITRKTFQLQAEPVVTPEQPSLLFLKYRNVQKLYFRTVKLTAARADELTARYNYGGDYQ
                     PEKIAAELVKSSPRHEWEIALPDDGDLREHRVEVPVPPLERGDLAILCGTDPQFSHDK
                     NRLDWVVIRSSRITFRHQVDERSLELFVADRTSGRPIAQAQVTIFRNEYDYQSSRYVE
                     RETASGTTDRNGILNISLPRNDRHSYGLSALITQGGDRLKSAFWGYPYSRDRRSFVRT
                     YFFTDRAIYRPGQTIYFKGIVIEYDAAGMPTIVAGKKDTVELYDPNGQKAGSAAVTSN
                     DYGSFHGSLTAPSGALNGRMTLRTKTGNHSVSVEEYKRPLFETTLEPPKGSYRLGDT"
BASE COUNT          550 a          800 c          718 g          412 t
ORIGIN      
        1 tagacgcggg gaacaacgac ggcgccatag gacttttcct acaggccatc gaactcgatc
       61 ccgccgatgg tgacgcttac tacggactcg gttacgccta tcacgcgaaa ggacagaagg
      121 acaaggcgcg gaactattac cagcgcgctc tgcagtcacg acttaaggaa gcggacaaag
      181 ccgacatccg cagtatcctc gaaaagatgt agtatttacc tcatatcctg ttcgcttctt
      241 gaccttttta cggggagggt ctcatggaac gacggttctt ctgggcgatg tccgttctcc
      301 tgcttctcgt ctcctgcggc ggatcaaaga gcgtcaccgg caccgatcct ctcgccgtca
      361 ccgacgaccc gaccgtaccc gacatagatt tcaccccgcc cgacaagtat gacgaccatg
      421 ccgcggagtg gaagaaggtc gagtcagccg aaaaggaggg aaaaccgcgt acggcgctcg
      481 aggagatagc gaagatactc gacgcggcgc gggcggaaca caatgtcccg cagatcgtca
      541 aagcgctcat tcagcgcgcg aagttcgaac agcgcatcgg cgaaaagcag ccggccgacc
      601 agatcgcgga gatcgagaag gagatagcgg cggcgcgctt ccctgaaaag aacctgcttc
      661 actccgtcgc ggccgatctc tacgaaagtt atttccagat gaaccgctgg cgtttctacg
      721 aacggaccgc cggggcgacc gagggggagg atatcgcctc gtggagtctg ccgaaactca
      781 tggcgacgat aacggcacat caccatgcgg cgctcgaaca cggcgcaaca ctgcgcgaga
      841 tcaagataga actccttgac gaggtactga ccaaaggtac gcgaccgcgg acgatccggc
      901 cgacgctgta cgatctcatc gcccaccgcg ccgccgattt cttcatgaac gacgagaact
      961 ctctcgccga aggggccgcc cccttcgagg tgcgtgacct cggctatctg ttccctgccg
     1021 aacggttcgc catcctcccg ataacgacga ccgaccggaa cgcgctcgcg tggcacgcgt
     1081 tgacgatctt gcaggatctc acccgcgccc atctacgcga caccgaccca gcggcgctcg
     1141 tcgacatcga actcaagcgg ctcgctttcg tccgctccat tcacaccgcc gataacaggg
     1201 acgatatcta tctgaagacg atggaactcc tcgaacagca atacggcgca cacgccgcct
     1261 cggcgagcat cggttacgaa cgggcccgct tttcgtacga gaaggggctc gcgtggcgcg
     1321 aagggatgcc cgacgcggag cggctggggc tcaaggaggc ggtcacgaaa tgcggcgcgg
     1381 tgacgctcaa gtggcccgac agcgaagggg cggcactctg tagggcgctc tcctccgaca
     1441 tcacccgcaa aactttccaa ctacaggcgg aaccggtggt gacacccgaa cagccatcgc
     1501 ttctgttcct gaaataccgc aatgtgcaga aactctattt ccgtaccgtg aaactgaccg
     1561 ccgcgcgggc cgacgaactg acggcgcgct ataactacgg cggcgactat cagccggaga
     1621 agatcgccgc agaactggtg aaatcgtcac cgcgccacga atgggagatc gcgcttcccg
     1681 acgacggcga cctacgcgag caccgcgtcg aggtgcccgt gccgccgctc gaacggggcg
     1741 atctcgcgat cctctgcggc accgatccgc agttctccca tgacaagaac cggctcgatt
     1801 gggtcgtcat ccggtcgtca cgcatcacct tccggcatca ggtcgacgag cggtcgttgg
     1861 aactgttcgt cgccgaccgg accagcggtc gtccgatagc gcaggcgcag gtcaccatct
     1921 tccggaacga gtacgactat cagtcctcgc gctatgtcga acgggaaacg gcgagcggaa
     1981 cgaccgaccg caacggtatc ctgaatatct ctctgccgcg caacgatcgc cattcctacg
     2041 gcctttcggc gctcatcacg cagggaggcg accggctgaa aagcgccttc tggggctatc
     2101 cctattcccg cgaccggcgt tcgttcgtcc gcacctattt cttcaccgac cgcgccatct
     2161 accggccggg acagacgata tacttcaaag ggatcgtcat cgaatatgac gcggcgggga
     2221 tgcccactat cgtcgcgggg aagaaggaca ccgtcgaact ctacgatccg aacggccaga
     2281 aggcggggag cgccgccgtc acaagcaacg attacggcag ttttcacggc agtctcaccg
     2341 cgccgagcgg cgccctgaac ggccggatga cgctacgcac caagaccggc aaccactcag
     2401 tttcggtcga ggaatacaag cggcccctgt tcgaaacgac gctcgagccg cccaaaggaa
     2461 gttaccggct cggcgatacg
//
LOCUS       DAOQUE010000425         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_51704_length_2470_cov_4.889855, whole genome shotgun sequence.
ACCESSION   DAOQUE010000425 DAOQUE010000000
VERSION     DAOQUE010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51704_length_2470_cov_4.889855"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2349
                     /locus_tag="PKH10_11345"
     CDS             <1..2349
                     /locus_tag="PKH10_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28756.1"
                     /translation="DPEKKVVVKNLTLTGGQRQGMYITGITAEISKIAIVSVMGQGLL
                     VDYGGTVSLSSAMVKGTLPLADKTRGRGIAAYDNISLFISKVLVERNKELGIMVATTE
                     TTSKSSFDATDLIVRNTASQESDLMWGRGVDISDAVTSTLSRVLVEKNRECGIFSGTW
                     TEGTRNSFTATHLIVRDTQSEEINKWYGRGITLRDNIDATLTHVLVERNREVGLSANT
                     FSDGAKGSMNVTDLIIRDTRSEEESQTLGDGLIVKDSVIAIINRALVERNREVGITIA
                     SSSAQTQGSFSATDLTVRNTLEKESDGYAGRGFWILDHPTVVLARTVVEGNRDIGIAI
                     SYSAPQEGKGGVLATDLVVRGTVSRTSDQQLGLGISIQDSVEADLDRVLIEQNQYIGL
                     QISTPSDGASGGIDATHLVVRDTGAQKSDAMFGRGISVQGAITSVFDKTLVKGNKEVG
                     LFAGSSTPESPCALSATDLIVIETQSQESDKMFGRGVVARDNVSASFSRALIDSNREV
                     GLVVRTDLPEATGSFDATDLVVRNTWPQESDQTVGRGIDITDNITANINRVLVEQNRD
                     VGIFVATHTDGVQGRFEGKDVMVRNTYAQESDKQWGSGIVFQDNIQSTLKNIAVENNR
                     ELGVQLINGDAAGVPIATFVNVIISDTMVRECYELDIDCGYALEAPFGHGLGIYYGAE
                     ATLVNVVIDGNQHGLQIKGSKVFAGENCSLPFGEEESETVCLHIINNETGINAFDLPA
                     DYDVFAAFANTSTWYEGNGTPFSGDPEDVPEPPDPINDPTEN"
BASE COUNT          650 a          542 c          681 g          597 t
ORIGIN      
        1 gacccggaaa agaaggtcgt ggtcaagaac ctgaccctca ccggtgggca gaggcagggg
       61 atgtacataa ccggcattac cgcagaaata tctaaaatag cgatcgtatc ggtcatggga
      121 caagggttat tggtagatta cggtggcaca gtgtcacttt cctctgccat ggtcaaaggt
      181 actctgccct tggcggataa aacgcgaggc cgcgggatag cagcatatga caatatttcg
      241 ctttttattt ccaaggtatt ggttgagcgc aacaaagagt tgggcattat ggtcgccaca
      301 acggaaacaa cctcaaaaag ttcttttgat gccaccgatc tgatagtgcg aaacaccgcg
      361 tcacaggaaa gcgatcttat gtggggcaga ggtgtagata tctcagatgc tgttactagc
      421 accctgtctc gcgttcttgt ggagaagaac agagagtgcg ggatcttttc tggtacatgg
      481 actgagggga cacggaattc ctttactgct acgcatctaa ttgttcgcga cacgcaatct
      541 gaagagatca acaaatggta cggtagaggg ataacactta gggacaacat cgatgcgaca
      601 ctcacgcatg tgcttgtgga aagaaataga gaggtcggtt tgtcagccaa cactttcagc
      661 gatggggcaa aaggtagtat gaatgtgacc gacctgatta ttcgtgacac acggtccgaa
      721 gaggagagcc aaacactagg tgacggtctg attgttaaag attccgttat cgccatcatt
      781 aaccgtgccc tcgttgagcg gaacagagag gtgggcatta caatcgccag ttcctctgcg
      841 cagacacagg ggagtttttc tgctaccgat cttaccgtgc gcaacacttt ggagaaagaa
      901 agtgatggct atgctgggcg aggattttgg atattggatc atccaacagt tgtccttgcc
      961 cgtactgttg ttgagggcaa tagagatatc gggatcgcta tttcctattc tgccccgcaa
     1021 gaaggcaagg ggggtgtact cgctaccgac cttgtcgtgc gcggcaccgt ttcgcgtacg
     1081 agtgatcaac agttgggatt gggcatttct attcaagaca gcgtggaggc cgaccttgat
     1141 cgtgtcctta tagaacaaaa tcaatatatc ggtcttcaga tatcaacccc aagtgatgga
     1201 gcaagcggag gcatcgatgc gacacacctt gttgttcgcg atacaggtgc tcagaaaagc
     1261 gatgctatgt ttggcagggg aatatctgtc cagggggcca ttacatcggt atttgacaaa
     1321 actttggtca aaggcaataa agaagtgggc ttgtttgccg gttcgtccac gccagaatcg
     1381 ccatgtgctc tttcggctac cgatctcatt gtgattgaaa cacaatcaca ggaaagtgac
     1441 aaaatgtttg gcagaggtgt ggtcgcccgg gacaatgttt cagcatcttt ttcccgtgcc
     1501 cttatagaca gcaatagaga ggtcggactc gttgtgcgga cggatctgcc tgaagcgacg
     1561 ggttcttttg atgccaccga tcttgtggtg aggaatacat ggcctcaaga gagcgatcaa
     1621 acggtaggca gaggtatcga tattacggac aacataaccg caaatattaa cagagtactt
     1681 gttgaacaaa acagagatgt cggtatcttt gttgcgacac atacagacgg tgtccaaggc
     1741 agatttgaag gcaaggatgt tatggtgaga aatacatatg ctcaggaaag tgacaagcaa
     1801 tgggggagcg ggatcgtttt tcaagacaac attcaatcga ccctaaaaaa tattgccgtt
     1861 gaaaacaatc gcgaactggg ggttcagctc attaatggtg acgccgcagg tgtgccgata
     1921 gcaacctttg ttaatgtgat catttctgac accatggttc gcgaatgtta tgaattggat
     1981 attgattgtg ggtatgcgct tgaagcgccg ttcggccatg gattgggaat atactatggc
     2041 gccgaggcga cactcgtgaa tgtggtgatc gatggcaatc agcatggcct gcagatcaag
     2101 ggatcaaaag tctttgcggg ggagaactgt tcgcttccct tcggtgaaga agagagtgag
     2161 accgtgtgcc tccatatcat caataacgag accggtatca atgcctttga ccttcccgcc
     2221 gactacgatg tctttgccgc ttttgccaat accagcactt ggtacgaagg taacggcacg
     2281 cctttttcgg gtgatcccga ggatgttccg gagcctccgg atccgatcaa tgacccgacg
     2341 gagaactaac acgaaggaga tgtcatgaga aagaagacgc tctttttcgc gatgatggcg
     2401 ttgctttcgc tttgcgcctg ctccgagaaa agcaccacgc ctaacgacga ggcggccacc
     2461 ggcgatgtcg
//
LOCUS       DAOQUE010000426         2469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_51748_length_2469_cov_4.487158, whole genome shotgun sequence.
ACCESSION   DAOQUE010000426 DAOQUE010000000
VERSION     DAOQUE010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51748_length_2469_cov_4.487158"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PKH10_11350"
     CDS             <1..752
                     /locus_tag="PKH10_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNT28757.1"
                     /translation="FFSVRLSDTPPDDDHPYGHGKIEGLSGMVEALLIFAAAGLIIFE
                     AVKKIMEGPEIAFPIAGVAVMALSAAINIVVSRRLYKVARETGSIALEADALHLKTDV
                     YTSAGVAIGLLLLWVTDIQLLDPIVAIAVACLILYESYQMLSKALSPLVDKRLSDGEI
                     ALIHEAIGRHHDSCIGFHEMRTRSSGNVRYVDLHLELPKYLTVEESHRICDAIETDIE
                     AKLPRTEVTIHVEPCSPETCGTCDHRTPRCG"
     gene            complement(756..1961)
                     /gene="tyrS"
                     /locus_tag="PKH10_11355"
     CDS             complement(756..1961)
                     /gene="tyrS"
                     /locus_tag="PKH10_11355"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNT28758.1"
                     /translation="MSDERTRHELDQLKSGIVDLYTEEELGKKLDRFHREGKPMVVKA
                     GFDPTSSDIHLGHTVLMNKMRQFQDLGHRVAYVVGDATAMIGDPTGRNKTRKVLTRDE
                     ILDHAKTYATQAFKILDPQKTEMVFNSTWLFKLDFMKLIDLASKYNVARMLEREDFKN
                     RFKSGDSISVHEFLYPLAQGYDSVALKADVELGGTDQIFNLLVGRKLQSDYGQEPQVV
                     MTVPLLVGLDGVQKMSKSYGNYIGVTEPADGMFAKVMSISDDLMWNYYELLTTVPVDE
                     IAAMKNGIQNDTIHPRDCKVNLALFITARFHGEPAAREAKENFYREIPSNLAEYPVTL
                     PDGGLALLDLIVQIGFTESKAEARRMIQQGGTYIDAQKVSDVALKITSKDSFVLKVGK
                     KKIGRVNFS"
     gene            complement(1954..>2469)
                     /locus_tag="PKH10_11360"
     CDS             complement(1954..>2469)
                     /locus_tag="PKH10_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HNT28759.1"
                     /translation="IHQPYGGVTGQAEDIKIGVVNLLGRVFMDPMDDPFSAADRELAA
                     LREAGAQVILIDFHAEATAEKEAFAFYLDGRAALLAGTHTHVQTSDEKILPGGMGYIT
                     DLGRCGSFLSVLGVEPKASIEGFLTGVPQNFTPAKSTLMMEGVLADIDPATGRTARIE
                     RIRQQAEAYDE"
BASE COUNT          435 a          756 c          743 g          535 t
ORIGIN      
        1 cctttttcag cgttcgtctc tccgacacgc cgcccgatga cgaccatccc tacggtcacg
       61 gcaagataga ggggctgtcg gggatggtcg aggcgctcct catcttcgcc gccgcgggac
      121 tcatcatctt cgaggcggtc aaaaagatca tggaaggtcc ggagatagcg ttcccgatag
      181 ccggtgtcgc ggtcatggca ctgtcggcgg cgatcaacat cgtcgtgtcg cgccgactct
      241 acaaagtggc gagggagacc gggtcgatcg cgctcgaggc cgatgccctc cacctcaaga
      301 ccgatgtcta cacctcggca ggcgtcgcta tcgggctttt gcttctttgg gtgaccgaca
      361 tccaactcct cgacccgatc gtggccatcg cggtcgcctg cctcatcctc tatgaatcct
      421 atcaaatgct cagtaaggcg ctgagtccgc tcgtcgacaa gcggctttcc gatggagaga
      481 tagcgctcat ccacgaggcg atagggcggc accacgattc gtgcatcggt ttccacgaga
      541 tgcggacccg cagttcgggg aatgtccgct atgtcgatct gcatcttgag ctgccgaaat
      601 atcttacggt cgaggaatcg caccgcatct gcgacgcgat agagaccgat atcgaggcga
      661 agttgccgcg gaccgaggtg acgatccatg tggaaccctg ctcccccgag acctgcggca
      721 cttgcgatca ccgcaccccg cgctgcgggt gaggtctaac tgaaattgac ccggccgatc
      781 ttcttcttgc cgaccttcag aacgaaggaa tcttttgagg ttatcttgag cgcgacatcg
      841 ctcaccttct gcgcgtcgat gtaggtgccg ccctgctgta tcatccggcg cgcctccgcc
      901 ttgctctcgg tgaacccaat ctgcacgatg agatcgagaa gcgcgagccc gccgtcgggc
      961 agcgtgacgg gatattcggc aaggttcgac ggtatctcgc ggtaaaagtt ctcctttgcc
     1021 tcacgcgccg ccggctcgcc gtggaaccgc gccgtgatga agagtgcgag gttcaccttg
     1081 cagtcgcgcg ggtggatcgt gtcgttctgt atgccgttct tcatcgccgc gatctcatcg
     1141 accggcacgg tggtgagcag ttcgtagtag ttccacatga gatcgtcgga gatcgacatc
     1201 accttcgcga acatgccgtc ggccggttcg gtgacgccga tgtagttacc gtaggatttc
     1261 gacatcttct ggaccccgtc gagccccacg agcagcggga ccgtcatcac gacctgcggt
     1321 tcctgcccgt agtcggactg caatttgcgc ccgacgagca gattgaatat ctggtcggtg
     1381 ccgcccagct ccacatcggc cttgagcgcg accgaatcgt agccctgcgc gagcggatag
     1441 aggaactcgt ggaccgatat agagtcgccg ctcttgaagc ggttcttgaa atcctcccgt
     1501 tcgagcatcc gagcgacatt gtacttcgag gcgaggtcta tgagtttcat gaaatcgagt
     1561 ttgaacagcc aggtcgagtt gaagaccatc tcggtctttt gcggatcgag gatcttgaaa
     1621 gcctgtgtcg cgtaggtctt cgcatgatcg agtatctcgt cacgggtaag caccttacgg
     1681 gtcttgttgc gcccggtcgg gtcaccgatc atcgcggtgg cgtcgccgac gacataggcg
     1741 acacggtggc cgaggtcctg gaactgacgc atcttgttca tgagcaccgt atggccgaga
     1801 tggatatcgc tcgaggtggg atcgaagccc gccttgacga ccatcggctt tccttcgcga
     1861 tggaaccggt cgagtttctt ccccagttcc tcttcggtgt agaggtcgac gatgccgctt
     1921 ttcaactggt ccagttcgtg gcgcgtccgc tcgtcactca tcatatgcct ccgcctgctg
     1981 tcgtatccgt tcgatccgtg ccgtccgtcc cgtcgccggg tcgatgtcgg ccagtactcc
     2041 ctccatcata agcgtgcttt tcgccggggt aaagttttgc gggacaccgg tcaggaaccc
     2101 ctcgatgctt gctttcggct cgacgccgag caccgagagg aacgatccgc accggccgag
     2161 gtcggtgata tagcccatcc cgccgggaag tatcttttcg tccgaggtct gcacatgggt
     2221 gtgggtcccg gcgagcaacg ccgcccgtcc gtcgagatag aaggcgaacg cctccttctc
     2281 ggcggtcgcc tcggcgtgga agtcgatgag gatgacctgc gcccccgcct cgcgcagtgc
     2341 ggccagttcg cggtcggccg ccgagaacgg gtcgtccatc gggtccatga agacgcgccc
     2401 gagcaggttg acgacgccga tcttgatgtc ctcggcctgg ccggtcactc cgccgtaggg
     2461 ctggtggat
//
LOCUS       DAOQUE010000427         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_52372_length_2450_cov_3.465136, whole genome shotgun sequence.
ACCESSION   DAOQUE010000427 DAOQUE010000000
VERSION     DAOQUE010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52372_length_2450_cov_3.465136"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="PKH10_11365"
     CDS             complement(<1..1650)
                     /locus_tag="PKH10_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT28760.1"
                     /translation="MKELIYQAVLNRRITLFFVAAITLYGLVSYYFLPKQENPDLDPT
                     IALVTAIYPGALPEDMEKLVTEKIEDAVSEIRGFDSVRSYSKNSISIVLVYIRSDADR
                     EKSWLDLRQKMDDLYPELPQGCWKPELNTKLGETAGLMISFSGGNYTYDQLEAYADIF
                     KERLRRIKGVVRFELAGKIDRVVRVTADAARLNQHRLSLEDLWKLLQAQNIEIPSGAI
                     ASDGSTINVSVPGKFASLRDIENTVIDVSTVDGSIVRLRDVATVSMGLDDEASKIRRN
                     GKEAVILAGYFEEKRNVLIIGDEVRAAIEEVKRSLPDDLTVDEVLYQPDDVRQAVNDF
                     MSNLLEGMLFVIIVVFVGMGFRNAMVVATAIPLSILFSYIAMGFFGIKVHQISTVALI
                     IALGMLVDNAIVISDAIQVKIDAGIDRVKAAYEGTVEVAVPVFTSTLTTVAGYYPLML
                     LPGNIGQYVLSVPQLVIIALSASYLVAMFVTPTMAVLWFRPSGGGTERPQPVRRLFER
                     LLTKGLDRPVAALLIVLGITLSALALGGLFLPIRFFPNADKN"
     gene            complement(1658..>2450)
                     /locus_tag="PKH10_11370"
     CDS             complement(1658..>2450)
                     /locus_tag="PKH10_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT28761.1"
                     /translation="LDPTDVGFAVSDAEHTLRKAKEAYDEAENFYQRIKALSEKGSVT
                     GDDHDKAKLNRDVREADYRRAQVDVDYKKNLAADLRMYSEVEGYVIELLNKPGEITGA
                     GYPVVVVRTDRQIVSVGVSQKDVKRLSIGMKALLSVDGVEGAGLISNIAQIPDLQSRT
                     YTVEIELADKLARSDFYLGSIAKVWFEVGEKEAVWVPVQAIQNDGLEYVYVVESGHAR
                     RRTVTLGAIEGTTVEVSGLEPGEVLVTEGMKDLHQGMPVAIVEQR"
BASE COUNT          440 a          803 c          729 g          478 t
ORIGIN      
        1 gttcttgtcg gcgttcggga agaaccgtat cggcaggaac agcccgccca gcgccagcgc
       61 cgaaagggtt atccccagca cgatcagcag cgccgctacc ggccggtcga gccctttggt
      121 cagtagccgc tcgaacagcc gccgcaccgg ctgcggccgc tccgtgcccc cgccggaggg
      181 acggaaccaa aggaccgcca tggtcggcgt cacgaacatc gcgacgaggt aggaggccga
      241 gagcgctata atgaccagct gcggcacgct gaggacatac tgcccgatgt tgcccggcag
      301 gagcatgagc ggatagtagc cggccacggt ggtgagggtg gatgtgaaga ccggcaccgc
      361 gacctcgacg gtcccttcgt aggccgcctt gacccggtcg atacccgcat cgatcttcac
      421 ctgtatcgcg tccgatatca cgatggcgtt gtcgacgagc atccccagcg cgatgatgag
      481 cgccacggtc gatatctggt ggaccttgat gccgaagaac cccatcgcta tataggagaa
      541 cagtatcgag agcggtatcg cggtcgcgac caccatcgcg ttgcggaacc ccatcccgac
      601 gaagacgact atgatgacga agagcatccc ttcgagcagg ttcgacatga aatcgttcac
      661 cgcctgcctc acatcgtcgg gctggtagag cacctcgtcg acggtgagat cgtcgggcag
      721 cgaccgcttc acctcctcta tcgccgcgcg cacctcgtcg ccgatgatga ggacattgcg
      781 cttctcctcg aaataaccgg cgaggatgac cgcctctttc ccattgcgcc gtatcttgct
      841 cgcctcgtca tcgagtccca tcgagacggt cgccacgtcg cggagccgca cgatggagcc
      901 atcgacggtc gagacgtcga tgacggtgtt ctcgatgtcg cggaggctcg cgaacttacc
      961 cggcacgctg acgttgatgg ttgagccatc gctcgcgatg gctccggagg gtatctcgat
     1021 attctgcgcc tgcagtagct tccagagatc ctcgagcgac agccggtgct ggttgagccg
     1081 cgcggcgtct gcggtgacgc gcacgacgcg gtcgatcttc cccgcgagtt cgaagcggac
     1141 gacccccttg atgcgtcgca gacgctcctt gaagatgtcg gcataggcct ccaactggtc
     1201 gtaggtgtag ttcccgccgg agaacgatat catgagcccc gccgtctcgc cgagtttggt
     1261 gttgagttcc ggtttccagc acccctgcgg cagttccggg tagagatcgt ccatcttctg
     1321 gcgcaggtcg agccacgact tctcgcggtc ggcgtcagac ctgatgtaga ccagcacgat
     1381 ggagatggaa ttcttcgaat aactgcggac gctgtcgaag ccgcgtatct cgctgacggc
     1441 gtcctctatc ttctcggtga ccagtttctc catgtcctcg ggcagggcgc ccgggtagat
     1501 cgccgtcacc agcgcgatgg tcggatcgag gtcggggttc tcctgtttgg gaaggaaata
     1561 gtaactgacc agaccataga gcgttatcgc ggcgacgaaa aaaagcgtga tcctgcggtt
     1621 cagcaccgcc tgatagatga gttccttcat ccccgtccta ccgctgttcg acgatggcga
     1681 ccggcatccc ctgatggaga tccttcatcc cctccgtcac gagcacctcg cccggctcaa
     1741 ggccgctcac ctcgaccgtc gtcccctcga tggccccgag cgtcacggtg cgccgccgcg
     1801 catgaccgct ctcgacgaca tagacatatt cgagcccgtc gttctgtatc gcctgcaccg
     1861 gcacccagac cgcctccttc tcccccacct cgaaccagac cttggcgatc gagccgagat
     1921 agaaatcgga gcgggccagt ttgtcggcca gctctatctc cacggtgtat gtgcgactct
     1981 gcaggtcggg gatctgcgcg atgttcgaga tgaggccggc cccctcgacc ccgtcgaccg
     2041 aaagcagcgc cttcatgccg atcgacagcc gcttgacatc cttctgcgac accccgacgc
     2101 tcacgatctg gcggtcggtg cggaccacca ccaccggata gccggcgccg gtgatctcac
     2161 ccggcttatt gagcagttcg atgacatagc cctcgacctc ggaatacatc cgcagatcgg
     2221 cggcgagatt cttcttgtaa tcgacatcga cctgcgcgcg ccggtagtcg gcctcccgca
     2281 catcgcggtt gagtttcgcc ttgtcgtgat cgtcgccggt gaccgacccc ttctcgctga
     2341 gcgccttgat gcgctgatag aagttctcgg cctcgtcgta ggcctccttc gccttgcgga
     2401 gcgtgtgttc cgcgtcggag accgcgaacc cgacgtcggt cgggtcgagt
//
LOCUS       DAOQUE010000428         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_52406_length_2449_cov_4.076441, whole genome shotgun sequence.
ACCESSION   DAOQUE010000428 DAOQUE010000000
VERSION     DAOQUE010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52406_length_2449_cov_4.076441"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKH10_11375"
     CDS             complement(<1..222)
                     /locus_tag="PKH10_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28762.1"
                     /translation="MRTALLVLLMLLLPALLAADEAKKKKVAVIDLIDDSATLDAAVV
                     ERATAYLWDRVVAANAFIVSPDSMLILWPR"
     gene            complement(227..2272)
                     /locus_tag="PKH10_11380"
     CDS             complement(227..2272)
                     /locus_tag="PKH10_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28763.1"
                     /translation="MNTPPRYWMLLAVVVSGAGAVYQAVSIRFLAYVFPFHMLFAISL
                     ISSLAIFFTGLGALSSRRWRDRHRPLTGLLLILFPLSAAAMNLADRVPFLAGNPVAAA
                     ILLTAPALFTAGALSGIAYHQTAVAAPERLKHLIALSALAFFAGYLLSVYLFTALGVW
                     NIFLATTLLLFLPLLWGRPAWSHAALLAAVLLLPAADMEGPAFRLFARDPHLWETAPG
                     RFVHGVWSPYARLDFYEMSDGRLAGLYNRTQQWAVGDPAFDLAVRRALYGRITGDVLV
                     IGSGGGYGLLSLTNASSITAVELDPGVVAAMRGPLASYNRNIYNTVKKVHAGDGRAFL
                     DGSDELFDAVILEAADLSYTAIPHSFVSMENYLYTKEGIATALSRLKPDGLLLIMVTK
                     ELIPAPKFVNALPAGVHWRLFEGEIEVIRSIRMNFDFILASPSADRIAAWEAILGDPA
                     LRLSPVGSSDLDRKSSFGLDPITDDRPLLYFKGWGQAVPFFALLFLLIATGVAVVSTA
                     ARRNEALFFSALGVAFMLAELHIINGMRAYLGGYLETSSILLGTLVLGGAAGTLAHDR
                     IGDRARTIGLALTIPLMLLLLRYAPLSWPFAAKALYLVAALVPAAFFMGTLFPAGLSR
                     SESEHAARHYATDTIGASLGLVLFYLLMLAGGFPLVALATLACYAAAVLLLTNIVKR"
BASE COUNT          461 a          739 c          924 g          325 t
ORIGIN      
        1 cctcggccac aggatcagca tcgagtccgg ggagacgatg aaggcgttgg cggcgaccac
       61 gcggtcccag agataggcgg tggcgcgttc gaccacggcc gcgtcgagcg tcgccgagtc
      121 gtcgatgagg tcgatgacgg cgaccttttt cttcttcgcc tcgtcggccg cgagcagggc
      181 gggcagaaga agcatcagga gcacaagaag cgcggtgcgc atggggtcac ctcttgacga
      241 tgttcgttaa aagaagcacc gccgccgcgt agcaggcgag cgtggcgagg gcgacgagcg
      301 ggaagccgcc cgcgagcatc agcagataga acagcacgag cccgagcgac gcgccgatgg
      361 tgtcggtcgc gtagtggcgc gcggcgtgtt cgctctccga tcgggaaagc ccggcgggga
      421 agagcgtccc catgaaaaag gcggcgggga cgagcgcggc gacaagatag agcgccttgg
      481 cggcgaacgg ccacgacagc ggcgcgtacc gcaggaggag gagcatgagc ggtatggtca
      541 gcgcgagccc gatggtgcgc gcccggtcgc cgatgcggtc gtgggcgagc gtcccggccg
      601 cgccgccgag cacgagcgtg ccgagcagta tcgacgaggt ctcgaggtag ccgccgaggt
      661 aggcgcgcat gccgttgatg atatggagtt cggcgagcat gaaggcgacg ccgagcgccg
      721 agaagaagag cgcctcgtta cgccgcgccg cggtggagac gacggcgacg ccggtcgcga
      781 taagcaggaa gaggagcgcg aagaagggga ccgcctgtcc ccagcccttg aagtagagga
      841 gcggccggtc gtcggtgatg gggtcgagcc cgaacgaact cttgcggtcg agatcgctcg
      901 acccgaccgg gctcaggcgg agcgccgggt cgccgagtat cgcctcccac gcggcgatcc
      961 ggtcggcgga gggggaggcg aggatgaagt cgaaattcat ccggatggag cggatcacct
     1021 ctatctcgcc ctcgaacagc cgccagtgga cccccgcggg gagggcgttc acgaatttcg
     1081 gggccggtat cagttccttg gtgaccatga tgaggagcag gccgtcgggc ttcagccgcg
     1141 aaagcgccgt cgcgatgccc tctttggtgt agaggtagtt ctccatgctc acgaaactgt
     1201 gcggtatcgc ggtgtaactg aggtcggccg cctcgaggat gaccgcgtcg aacaactcgt
     1261 cggacccgtc gaggaaggcg cggccgtcgc ccgcgtggac ctttttcacg gtgttgtaga
     1321 tgttgcggtt ataggaggcg agcggtcccc gcatcgccgc gaccaccccc gggtcgagtt
     1381 cgaccgccgt tatcgaggag gcgttcgtga gggacaggag tccgtagccg ccgcccgacc
     1441 cgatgacgag cacatcgccc gttatcctgc cgtacagcgc gcggcgcacc gcgaggtcga
     1501 acgccgggtc gccgaccgcc cactgctgag tgcggttgta cagccccgcg agccgcccgt
     1561 cgctcatctc gtagaaatcg agccgcgcat agggcgacca gaccccgtgc acgaaccggc
     1621 ccggcgccgt ttcccagagg tgcggatcgc gggcgaagag ccggaaggcc ggtccctcca
     1681 tgtcggcggc gggcaggagc aggacggcgg cgaggagcgc cgcgtgcgac catgccgggc
     1741 ggccccacag gagcggcagg aacaggagca gcgtcgtcgc gaggaatatg ttccacacgc
     1801 cgagcgcggt gaacaggtac accgacagca ggtacccggc gaaaaaggcg agcgccgaca
     1861 gggcgatgag gtgcttgagc cgctccggcg cggcgaccgc cgtctggtgg taggcgatgc
     1921 ccgacagcgc gccggcggtg aagagggcgg gggcggtgag caggatcgcg gcggcgaccg
     1981 ggttgcccgc gaggaacggg acgcggtcgg cgaggttcat cgcggcggcc gacagcggga
     2041 aaaggatcag caggagcccg gtgagcggcc ggtgccggtc gcgccagcgg cgcgacgaga
     2101 gcgccccgag cccggtgaag aatatcgcga gcgacgatat gagcgatatc gcgaagagca
     2161 tgtggaacgg gaacacatag gcgaggaacc gtatcgacac cgcctgatag accgccccgg
     2221 cgcccgaaac gacgacggcg agcagcatcc agtagcgggg gggcgtgttc atcggcgact
     2281 cacatgcagt ccttttcggt gcagaggccg tcgacattga gatcctcgat cgccgggtcg
     2341 cacagcccgt tctggttcgc gtcccagcag cggacgatgt cggcgaggtc gagcccggcg
     2401 gtgtaggcgt aggagacata ctgcgagaag ccgtacacat agaggccga
//
LOCUS       DAOQUE010000429         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_52606_length_2443_cov_3.676717, whole genome shotgun sequence.
ACCESSION   DAOQUE010000429 DAOQUE010000000
VERSION     DAOQUE010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52606_length_2443_cov_3.676717"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKH10_11385"
     CDS             <1..342
                     /locus_tag="PKH10_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28764.1"
                     /translation="EEAKISLAYLPLGTTEGAPIRIGESYLKDERGETVVSKLNREFF
                     DKCRDGLGAIEITRGETLASFLRSFRNRAGTDEKRVQLYREQFPVPLALGLLFFTLFI
                     VLPSGRKEEKK"
     gene            339..1121
                     /locus_tag="PKH10_11390"
     CDS             339..1121
                     /locus_tag="PKH10_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT28765.1"
                     /translation="MNRATLFLTALLAFSFSPFQREIPENRDGTAKYEQQQYGEAGES
                     FEKGAERIEDSPELSYNLGAALMKKGDLDGARKAWEKALAQSPAPELKGRIYDGLGAL
                     SANRQQYQEAAEFFKRSLRNHPDRDTAANLEIVRRLLQQQQQQEQKQQEQKQDQQQQQ
                     QEQKQDQQDQQDQQQQQQEQKQDQQDQQQQQQQQQQQQQQQQEEKKEEQQQAAQAEKK
                     EKEEKDKKDEKQTLAPFRQRKNLQITPFMLEKQGDPQGGQTW"
     gene            1118..>2443
                     /locus_tag="PKH10_11395"
     CDS             1118..>2443
                     /locus_tag="PKH10_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNT28766.1"
                     /translation="MRTGLLFISLSLLWGSLSATSARMEADRQSLSVGDIVSLVLTIV
                     DDEKEDVVEVKEPELPGFEIEDRGESTSSSVSIINGKVSSRKERRITYVLRARRPGQF
                     TLGPAILVLEKGREIRSDQVRVTVTGTASPGSPPTPGAPAGEEPGSAASDAGGELFAP
                     LSAWEKQTGHFFVRVTVSPAAPRHQGEPLIVSYYLFTQKNLISDLSFYRLPSFENAWS
                     EEIESPRKLSFGRTNIGGTVYDHALLKRYVIVPEKNAVQVGATQMILEVMTGSFFDAR
                     KRTLSSIALSLDLTPLPDADRHPGGIVGDLSITQDRTSMELGKEKPLDTLTYTVTGCG
                     DLHHLDLAPPPVDGLKIFAPDTTTDQRFDGTRLCGVKTFKFMVKAQRTGDIEIPAVTL
                     SLYDRDKGWGTVASRPVTVRVGELATATDEAKEAKKTVRYELLRELPAGT"
BASE COUNT          599 a          750 c          727 g          367 t
ORIGIN      
        1 gaagaggcaa agatctctct cgcctaccta ccgctgggca ccaccgaggg ggcgccgata
       61 cgcatcggcg agagttatct caaggatgaa cgaggcgaaa cggtcgtgtc gaaactgaac
      121 cgcgagttct tcgacaagtg ccgcgacgga ctcggcgcga tcgagataac gcgcggcgaa
      181 acgctggcat cgttcctcag gtcattccgg aatcgcgccg ggaccgatga gaagcgtgtg
      241 caactgtacc gcgaacagtt cccggtgccg ctcgcgctcg gcctgttgtt cttcaccctc
      301 ttcatcgtcc tgccgagtgg tcgcaaggag gagaaaaaat gaaccgggcg accctgttcc
      361 ttacggcgct tctcgccttt tcgttctcgc ctttccagcg ggagatcccc gagaaccggg
      421 acggcaccgc gaaatatgaa caacagcagt acggcgaggc aggcgaaagt ttcgagaaag
      481 gggccgaacg gatcgaggat tcacccgaac tttcctacaa cctcggcgcg gcgctgatga
      541 agaagggcga cctcgacgga gcgcggaagg cgtgggaaaa ggcgctcgcc cagagcccgg
      601 cacccgaact caaagggcgc atctacgacg ggctcggcgc gctttcggcg aacagacagc
      661 agtatcagga ggcggcggag ttcttcaaac ggtcgctccg gaaccatccc gaccgagaca
      721 ccgccgccaa tctggagata gtgcgtcggc tgttgcaaca acagcaacaa caagaacaaa
      781 aacaacaaga gcagaaacag gaccaacaac agcaacaaca agaacagaag caggaccagc
      841 aggaccagca ggaccagcag cagcaacagc aagaacagaa gcaggaccag caggaccagc
      901 agcagcaaca gcaacaacag cagcaacaac agcagcaaca gcaggaagag aaaaaagagg
      961 aacaacaaca ggcggcacaa gccgaaaaga aagagaaaga ggaaaaggac aaaaaagacg
     1021 agaagcagac gctcgcgccg ttccgccagc gcaaaaattt acaaatcacc cccttcatgc
     1081 tagagaaaca gggcgatccg cagggaggcc agacctggtg agaaccggct tacttttcat
     1141 atcgttatca ctcctctggg gttccctgtc ggccacctcc gcccggatgg aggccgaccg
     1201 gcagagtttg tcggtaggcg acatcgtgtc gctcgtgctc accatcgttg atgatgagaa
     1261 ggaggatgtg gtcgaggtca aagagccgga actgcccgga ttcgagatag aggatcgcgg
     1321 cgaatcgacc tcctcgtcgg tcagcatcat caacgggaag gtgagttccc gcaaggaacg
     1381 ccgtataacc tatgtactcc gcgcccggag accgggccag ttcacgctcg gtccggcgat
     1441 cctcgtgctc gagaaggggc gggagatccg gtcggatcag gtgcgtgtca ccgtcaccgg
     1501 caccgcgtcc cccggctcac caccgacacc cggcgcgccg gcgggcgaag aacccgggtc
     1561 ggcggcatcg gacgcggggg gcgaactgtt cgcgccgcta tcggcgtggg agaaacagac
     1621 cggacatttt ttcgtgcgag tgacggtgtc tcccgccgcg ccgcgccatc agggcgaacc
     1681 gctcatcgtg agttattacc tcttcacgca gaagaacctc atatcggatc tttcgttcta
     1741 ccggcttccc tcgttcgaga acgcatggag cgaagagatc gagtcgccgc gcaaactctc
     1801 cttcggccgc accaacatag gcgggacggt gtacgatcac gccctgctca aacgatatgt
     1861 catcgtgccg gaaaagaacg cggtgcaggt gggcgcgacg cagatgatcc tcgaggtcat
     1921 gaccggcagt ttcttcgacg cccgcaaacg gaccctctcc tcgatagcgc tttccctcga
     1981 cctgacgccg cttcccgatg ccgatcgtca ccccggtggc atcgtcggcg atctttcgat
     2041 aacacaggac cggacctcga tggaactcgg caaggaaaag ccgctcgaca ccctcaccta
     2101 taccgtgacg ggctgcggcg accttcacca cctcgatctc gccccaccgc ccgtggacgg
     2161 tctcaagata ttcgcccccg acaccacgac cgaccaacgc ttcgacggca cgcggctctg
     2221 cggcgtaaag accttcaaat tcatggtcaa ggcccagcgc acgggggata tcgagatacc
     2281 ggcggtgacg ctttccctct acgaccggga caaggggtgg ggtactgtgg catcacgacc
     2341 ggtcacggtc cgcgtcggcg aactcgccac agcgaccgat gaggcgaaag aggcaaaaaa
     2401 gacggtgcgg tacgaactgc tacgcgaact gccggcgggg acc
//
LOCUS       DAOQUE010000430         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_52679_length_2441_cov_3.667225, whole genome shotgun sequence.
ACCESSION   DAOQUE010000430 DAOQUE010000000
VERSION     DAOQUE010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52679_length_2441_cov_3.667225"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /gene="udk"
                     /locus_tag="PKH10_11400"
     CDS             complement(<1..458)
                     /gene="udk"
                     /locus_tag="PKH10_11400"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNT28767.1"
                     /translation="MPLVIGIAGGTGSGKSTIAHNIKAAVAPQETVAIIEMDAYYKDH
                     SHLSDTDREALNYDHPDSIDFELFAKQLKAIIAGQAIEKPIYDFVKHGRRADIDLTEP
                     ADIVIVEGILAFHRKEIRDLFDIKLYVDTDPDIRAFRRIRRDIEVRGRKF"
     gene            complement(480..1676)
                     /locus_tag="PKH10_11405"
     CDS             complement(480..1676)
                     /locus_tag="PKH10_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT28768.1"
                     /translation="MITASKRGAAMPPSPIRKLKPYADKAIAAGKTVYQLNIGQPDIE
                     TPPAMFDALKKLGTKVIAYGPSQGIPEYQDALIKYYKKHGIELGRENIIVTNGGSEAI
                     IMGMTVCCDPGDEILVPEPFYTNYNGFATQAAVTVRPITTRIEDDWELPDISVIERLI
                     TPKTKALMICNPNNPTGKLYGEEELRKLAYLAKKHNLFLFGDEVYREFIFSGEKHTSL
                     LEFRDMDEHVVMMDSISKRFSACGSRIGAFVSRNKAVMQAALCFGQARLCPPTIDQLM
                     AVPTVELGKEYFDTMVGEYKKRRDAVLEGLAKMADVEYKVPKGAFYIVMRLPVADAED
                     FVIWMLTEFQVNNETVMLAPAEGFYATPGLGRNEARIAFVLNVEKTKKAMEILRLALE
                     AYRKKC"
     gene            complement(1774..>2441)
                     /locus_tag="PKH10_11410"
     CDS             complement(1774..>2441)
                     /locus_tag="PKH10_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28769.1"
                     /translation="LQSILDYSRSPGTTGSAAIDPLFNATKIQNEACEDDYPWYWSGT
                     THAAYNGSGASGAYVCFGRALGYMQSTWIDIHGAGAQRSDPKGGSLSTNYTAQDCGYY
                     NPIAPQGDGVRNSNFVRLVRSGEATFTTETDEPDNDNTPSDSDNVQPDADIPTGPTSC
                     DIDDDCAVVGACPPGALMGCGCRETPSGDKVCMPLCENSDDCPVDMNGNAMICDNEIC
                     RPA"
BASE COUNT          410 a          696 c          777 g          558 t
ORIGIN      
        1 tcgaacttgc ggccgcgcac ctcgatgtcg cggcggatgc ggcggaaggc gcggatgtcg
       61 gggtcggtgt ccacatacag ttttatgtcg aacagatcgc gtatctcctt gcggtggaac
      121 gcgagtatcc cctcgacgat gacgatgtcg gccggttcgg tgagatcgat atccgcgcgg
      181 cgtccgtgct tcacgaaatc gtagatcggc ttttctatcg cctgcccggc gatgatcgcc
      241 ttcaactgct tggcgaatag ttcgaagtct atcgagtcgg gatggtcgta attgagcgcc
      301 tcccggtcgg tgtccgacag gtgcgaatga tccttgtagt aggcgtccat ctcgatgatg
      361 gcgaccgttt cctgcggcgc cacggccgcc ttgatgttgt gggcgatggt cgacttgccc
      421 gagccggtcc ctccggctat cccgatgacg agcggcatgg tatcctcctc gttatatcct
      481 caacatttct tgcgataggc ctcgagggcg agccgcagta tctccatcgc ctttttcgtc
      541 ttctcgacat tgaggacgaa cgcgatgcgc gcctcgttgc ggccgagccc cggggtggcg
      601 tagaacccct cggcgggggc gagcatgacc gtttcgttat tcacctggaa ttcagtcagc
      661 atccagatga cgaaatcctc cgcgtcggcg accggcaggc gcatcacgat gtaaaaggcc
      721 cccttcggca ccttgtactc gacatcggcc atcttggcga gcccttcgag caccgcgtcg
      781 cggcgttttt tgtattcgcc gaccatcgtg tcgaaatact ccttgccgag ttctaccgtc
      841 gggaccgcca tgagttggtc tatcgtcggc gggcagagtc gcgcctgacc gaagcagagc
      901 gccgcctgca tcaccgcctt gttgcggctc acgaacgcgc cgatgcgcga gccgcaggcc
      961 gagaagcgct tcgagatgct gtccatcatc acgacatgtt cgtccatgtc gcggaactcg
     1021 aggagcgagg tgtgtttttc gccgctgaat atgaattcgc ggtagacctc gtcgccgaac
     1081 aggaacaggt tgtgtttctt ggcgagatag gccagtttgc gcagttcctc ttcgccgtag
     1141 agtttgccgg tggggttgtt cgggttgcag atcatgagcg ccttcgtctt cggggtgatg
     1201 agccgctcga tgaccgatat gtcggggagt tcccagtcat cctctatccg cgtggtgatc
     1261 gggcgcaccg tcaccgccgc ctgtgtcgcg aagccgttgt agttggtgta gaacggttcg
     1321 gggaccagta tctcgtcgcc cgggtcgcag cagacggtca tccccatgat gatcgcttcg
     1381 ctaccgccgt tcgtcacgat gatgttctcg cgtcccagtt cgatgccgtg ttttttgtag
     1441 tatttgatga gggcgtcctg atattcgggg atgccctgcg acgggccgta ggcgatgacc
     1501 ttggtcccga gttttttcag cgcgtcgaac atcgcggggg gcgtctcgat gtcgggctgg
     1561 ccgatgttga gttgatagac ggtcttcccc gccgctatcg ccttgtcggc gtagggcttc
     1621 agtttgcgga tgggggaggg cggcatcgcg gcgccgcgtt tagaggcggt tatcatgtca
     1681 tactccggca aatgatgaac aaacaccctg aaggtgtagc cgcggcgggc gcaaaaggca
     1741 agaaatttcg gtatggcttt gggatgaccg gcttcaggcg gggcgacaga tctcgttgtc
     1801 gcagatcatg gcgttcccgt tcatgtcgac ggggcaatcg tcgctgtttt cgcagagcgg
     1861 catacagacc ttgtcgccgg agggggtttc ccgacaaccg catcccatca gggcgccggg
     1921 aggacaggcg cccactacgg cgcagtcatc gtctatgtca caactggtgg gaccggtggg
     1981 aatgtcggca tccggttgca cattgtcgct gtcagaaggc gtgttatcgt tatccggctc
     2041 atcagtttcg gtcgtaaagg tagcctcacc gctgcgcaca aggcggacga agttcgaatt
     2101 gcgcacgccg tcaccctgcg gagcgatcgg gttatagtag ccgcagtcct gtgccgtgta
     2161 gttggtggag aggctaccgc ccttaggatc cgagcgttgc gcgcccgcgc cgtggatgtc
     2221 gatccacgtg ctctgcatgt agccgagcgc cctgccgaag cagacatacg cgccggaggc
     2281 ccccgaaccg ttgtacgccg cgtgcgtcgt gccgctccag taccaaggat agtcgtcctc
     2341 gcacgcttcg ttctgaattt ttgtggcgtt gaagagcgga tcgatggcgg ccgagccggt
     2401 cgttcccgga gaacgggaat agtcgagaat gctctgaagc t
//
LOCUS       DAOQUE010000431         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_52791_length_2438_cov_3.787243, whole genome shotgun sequence.
ACCESSION   DAOQUE010000431 DAOQUE010000000
VERSION     DAOQUE010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52791_length_2438_cov_3.787243"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKH10_11415"
     CDS             complement(<1..229)
                     /locus_tag="PKH10_11415"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNT28770.1"
                     /translation="MTVPLLVLSGLDPTGGAGLTADVAMARSLGLHPLPVPTVLTVQN
                     SLRFSGMRPVEPAYIRSAVESLAEEFRFDVAK"
     gene            complement(226..522)
                     /locus_tag="PKH10_11420"
     CDS             complement(226..522)
                     /locus_tag="PKH10_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNT28771.1"
                     /translation="MDPLLTERGLEVKVPPLLRGMSSRDYFQRLEESVRDMNGNIYFD
                     CSDATLIDSTALGKIIFLAKQLQRRNQKVVFESCPVTIRDFLTKLKVNNFIDIA"
     gene            complement(601..2052)
                     /gene="gltX"
                     /locus_tag="PKH10_11425"
     CDS             complement(601..2052)
                     /gene="gltX"
                     /locus_tag="PKH10_11425"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNT28772.1"
                     /translation="MTVRTRFAPSPTGYMHVGNLRTALYAYLLARKHNGVFILRIEDT
                     DQEREVAGAVDIIYHTLRQVGLLWDEGPDVGGPCGPYIQSERKPIYREYADKLVESGH
                     AYYCFCDKERLDMMRKRQEAEGKQPKYDGHCRTLSREEREELIAGGRSWVIRQRIPDE
                     GMTTFKDEVFGEITVENATLDDNVLLKSDGLPTYNFANVVDDHLMGISHVIRGIEYLS
                     SSPKYNLLYTAFGWEIPKYVHCPPVMRDAQKKLSKRDGDASYQDFIDKGYLNEALINY
                     LALLGWNPGNDREKFTLPELIEAFSIEGISRSPAIFDPVKLAWLNGEYLRALTPEEFT
                     AKAIPFIRKTVKREDVPLPAIAAVLHQRTEKLSDIPPQLDFIDTLPAYSKELFLHKKS
                     KTTFENSLASLRAAREALAAIPPENWDHATLHATLMGLVEKLGVKNAIVLWPVRVALS
                     GKEFTPGGGIEIAHILGKDEALHRLDAGIEKLS"
     gene            complement(2049..>2438)
                     /locus_tag="PKH10_11430"
     CDS             complement(2049..>2438)
                     /locus_tag="PKH10_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28773.1"
                     /translation="FMIYYLLGSPHFVAVEAMPEEKDGEEENLGGTMVTIRGRAYGFQ
                     TDRGAKRFATFFEKNYGITMAEKGWLLRAGVEPRTDLFPVFLWALLLVLFAANIVLAI
                     KTLRRNTSEEGEGSGEEDGPEDDGEET"
BASE COUNT          464 a          721 c          727 g          526 t
ORIGIN      
        1 tcttggcgac atcgaaccgg aactcctcgg cgagcgactc cacggcggac cggatatagg
       61 ccggctcgac gggacgcatc cccgaaaaac ggagcgagtt ctggaccgtc agcacggtcg
      121 ggacaggaag cggatggagg ccgagggagc gggccatggc aacatcggcc gtcagtccgg
      181 cgcccccggt cgggtcgagc ccggagagca ccagcaacgg gacggtcatg cgatgtcgat
      241 gaagttgttg accttgagtt tggtgagaaa gtcgcggatc gtaacagggc acgattcgaa
      301 caccaccttc tggttgcggc gctggagttg tttggcgaga aagatgatct tgccgagcgc
      361 cgttgaatct atcagggtcg catccgagca gtcgaagtag atgttgccat tcatgtcgcg
      421 gacactctcc tcgagccgtt gaaagtaatc gcgcgaggac attcccctca aaagcggcgg
      481 gaccttgacc tcaagacctc tctctgtgag caacggatcc atcttaacac acctcccgga
      541 tatttttttg ttcttcaacg gaagcgactc tagccacccg cagagtgaaa agcaaatttt
      601 ttacgacaac ttttctatgc cggcgtccag gcgatgcaac gcctcgtctt taccgaggat
      661 atgggctatc tcgatgcctc cgccgggggt gaattccttg cccgacagcg cgacacgcac
      721 cggccacagg acgatggcgt tcttgacccc gagtttctca acgagcccca tgagcgtcgc
      781 atggagcgtc gcgtggtccc agttctccgg cggtatcgcc gcgagcgcct cgcgagcggc
      841 acggaggctc gcaagcgagt tctcgaaggt ggtcttcgac ttcttgtgca ggaagagttc
      901 tttcgagtag gcgggcaggg tgtctatgaa gtcgagttga ggcgggatgt ccgaaagttt
      961 ctcggtccgc tggtggagca ccgcggcaat ggctgggagc ggcacatcct cgcgcttcac
     1021 cgtcttccgg atgaagggga tcgccttggc ggtgaactct tctggggtca gggcgcgcag
     1081 atattcgccg ttgagccacg ccagtttcac cgggtcgaat atcgcgggtg atctggatat
     1141 gccctcaatg gaaaaggcct cgatgagttc gggaagggtg aacttctcgc ggtcgttccc
     1201 cggattccaa ccgaggagcg cgagataatt gatgagcgcc tcgttgagat agcctttgtc
     1261 gatgaagtcc tgataactcg cgtcgccgtc gcgcttcgac agttttttct gggcatcgcg
     1321 catgaccggc ggacagtgga catacttcgg tatctcccag ccgaacgcgg tgtagagcag
     1381 gttgtacttc ggcgacgaag agagatattc gatgccgcgg atgacatggg atatccccat
     1441 caggtgatcg tccaccacat tggcgaagtt ataggtcggc aggccgtccg atttgaggag
     1501 gacattgtcg tcgagcgtgg cgttctccac ggtgatctcg ccgaagacct cgtctttgaa
     1561 ggtcgtcatc ccctcgtcgg ggatccgctg gcggatgacc cacgatctac ctccggcgat
     1621 gagttcttcg cgctcttcgc gcgacagggt gcgacagtgg ccgtcgtatt tcggctgttt
     1681 tccctccgcc tcctgccgtt ttctcatcat atcgagccgt tccttgtcac agaaacagta
     1741 gtaagcgtgg ccgctctcga ccagtttgtc ggcatattcg cggtatatcg gcttgcgctc
     1801 gctctgaatg tagggaccgc acggaccgcc cacgtcggga ccttcgtccc acagaagccc
     1861 cacctgtcgc agggtgtggt agatgatgtc gaccgccccc gcgacctcgc gttcctgatc
     1921 ggtgtcctct atccggagga tgaagacacc gttatgtttt ctcgccaaca gataggcgta
     1981 cagggcggtc ctcaagttgc cgacatgcat gtaaccggtc gggctcgggg cgaaacgggt
     2041 gcgtacggtc atgtctcctc tccgtcgtct tcgggtccat cctcttcgcc gctcccctcc
     2101 ccctcctccg atgtgttccg ccgcagggtc ttaatcgcca gaacgatgtt cgccgcgaac
     2161 aacaccagaa gcaaggccca caagaagacc gggaacaggt cggtgcgcgg ttcgacgccc
     2221 gcccgcagga gccacccctt ctcagccatc gttattccgt agttcttctc aaagaaggtt
     2281 gcgaagcgtt tcgctccccg gtcggtctgg aagccgtacg cacggccccg gatggtgacc
     2341 atggtcccgc cgagattctc ctcttcgcca tccttttcct ccggcatcgc ctcgaccgcc
     2401 acaaaatgcg gcgaacccag taggtagtag atcatgaa
//
LOCUS       DAOQUE010000432         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_52959_length_2433_cov_4.615223, whole genome shotgun sequence.
ACCESSION   DAOQUE010000432 DAOQUE010000000
VERSION     DAOQUE010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52959_length_2433_cov_4.615223"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PKH10_11435"
     CDS             complement(<1..903)
                     /locus_tag="PKH10_11435"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNT28774.1"
                     /translation="MRRQILVNAIPGEKRLAVLENDRLVEFSIQRDHDHRCLGNVYRG
                     TVKRIVHGMQSAFIEFGHDRTGFIYLRDLRQDISYEEFEKNLNDDEDNGDTATAAPPV
                     PEYVREGEPIIVQVVKEPIGQKGARLTTHVTLPGRFAVLMPTIRHTGVSKKITDPEVR
                     HDMQDLARRTSEKGYGVIIRTMAGSAPRQIVEREITQLIKKWRAIQTNFKKAKTDGLI
                     VEAVSPLIEAIKNTYTDDLDRVVCDSAEDHEEALAYVSDFIPEKRSVVQRHDLPYPIF
                     EYYSVEADIERTLGRKLWLKSGGFI"
     gene            complement(905..1675)
                     /locus_tag="PKH10_11440"
     CDS             complement(905..1675)
                     /locus_tag="PKH10_11440"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNT28775.1"
                     /translation="MLSILPLGCYGSDLEEKKCISMRLSPSTIIDAGAILSNMSSAEL
                     LKVKHIIITHAHFDHIKDLPTLADFMLSFGMHTFTIHTTRTIADQIRQYVFNDLIWPD
                     FTNLPKKGTPTVAFEYFEYGRPFVIEDMEILPIPVNHVVESVGFIIRQDGTSLGYSGD
                     TGPCDRFVEAVNNEKNLQLLCWETSFPNRLEAIAKNSKHLTPHGLESELARVSPQVPV
                     WVFHMKPSMIDEIEKELSEIKTPKPIRFMRQKRPIVVG"
     gene            1847..2419
                     /locus_tag="PKH10_11445"
     CDS             1847..2419
                     /locus_tag="PKH10_11445"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNT28776.1"
                     /translation="MEKKRIRKDTLERRRLLSAAELSEKSHDIVLRIAGTTLFRVAED
                     VAVYHPYRGEADLLPLLGTPGKRFHFPRVVDGTKVLAFHRIGSPEELIDGFKGIREPA
                     TDAPHCPVADIDLFLVPGVAFSRNGERVGYGGGYYDATLRERGPRSTAIGVGFDLQVV
                     EPGFADPWDARLDGLVTESSVMFINITEKE"
BASE COUNT          457 a          682 c          753 g          541 t
ORIGIN      
        1 gatgaatccg ccgcttttca gccacagttt cctgccgagc gtccgttcga tgtcggcctc
       61 gacgctgtag tactcgaata tcggataggg caggtcatgc cgctggacta cagaccgttt
      121 ttctggaatg aaatcggaaa catatgcgag cgcctcctca tggtcctccg cgctgtcgca
      181 gaccacccgg tcgagatcgt cggtgtaggt gttctttatc gcctcgatga gcgggctcac
      241 cgcctcgacg atcagcccgt cggtcttcgc cttcttgaag ttcgtctgta tggcgcgcca
      301 tttcttgatg agttgcgtta tctctcgttc gacgatctgg cgcggcgccg agccggccat
      361 ggtacggatg atgacgccgt atcccttctc gctcgtcctc cgggcgagat cctgcatatc
      421 gtgccgcacc tcggggtccg ttatcttctt ggaaacgccg gtgtgacgga tggtgggcat
      481 gaggaccgcg aaccggcccg ggagcgtcac atgggtcgtg agccgcgccc ccttctgccc
      541 gatgggctcc ttgacgacct gcacgattat cggctcgccc tcccggacat attccggcac
      601 cggcggcgcg gcggtggcgg tgtcgccgtt atcttcgtcg tcgttcaggt tcttctcgaa
      661 ctcctcgtac gatatgtcct gccgcaggtc gcgcaggtag atgaagccgg tccggtcgtg
      721 gccgaactcg atgaaggccg actgcatgcc gtggacgatg cgtttcaccg tgccgcgata
      781 gacattgccg aggcaccggt gatcgtggtc gcgctgtatc gagaattcga cgagccggtc
      841 gttctcgagg acggcgagcc gtttctcgcc cggtatcgcg ttgacgagga tctgacggcg
      901 catgctaccc gaccacgatc ggccttttct ggcgcatgaa gcggatgggt tttggggtct
      961 tgatctcgct gagttccttt tctatctcgt ctatcatgct gggcttcatg tggaacaccc
     1021 agaccgggac ctgcggggag acccgtgcca gttcggactc gagaccgtgg ggcgtcaggt
     1081 gcttggaatt cttcgctatc gcctccagcc ggttggggaa cgaggtctcc cagcagagca
     1141 gctggagatt tttttcgttg ttgaccgcct cgacgaaccg gtcgcagggg ccggtgtcgc
     1201 ccgaataccc gaggctcgtc ccgtcctgtc ggatgatgaa cccgaccgat tcgacgacat
     1261 ggttcaccgg tatcggcagt atctccatat cctcgatgac aaagggacga ccgtactcga
     1321 aatactcaaa ggcgacggtc ggggtcccct tcttgggcag gttggtgaaa tcgggccaga
     1381 tgagatcgtt gaacacatac tgccgtatct ggtcggcgat ggtacgggtc gtgtggatgg
     1441 tgaaggtgtg catgccgaac gacagcatga agtcggcgag cgtcggaaga tccttgatgt
     1501 ggtcgaagtg ggcgtgggtg atgatgatgt gcttcacctt gagcagttct gccgaggaca
     1561 tgttggaaag aatggcgccg gcatcaatga tggtggaggg agaaagacgc atcgagatac
     1621 atttcttctc ctcgaggtcg ctcccgtaac acccgagcgg cagtatggac agcacgcgag
     1681 atcgtctcct caaaaacaac ggggcatcct acagcgaccg gaaggcggtg tcaagaaata
     1741 aaatttgaca tttctctccc tttttgatac taagtgtctt tcccgctcat gcgggtttac
     1801 atagcgtgtt taacaatggt cgaaagccgc aaggcttatc gatatcgtgg aaaaaaagcg
     1861 aattcgaaaa gataccttgg aacgccggcg actcttgagt gcggcggaac tgtccgaaaa
     1921 gagtcatgat atcgttttgc gtattgcggg aacgaccctg ttccgcgtcg cggaggatgt
     1981 cgcggtgtat cacccgtacc ggggagaggc cgatcttctg ccgcttctcg ggacgcccgg
     2041 taaacggttc cattttcccc gggtggtcga cggaacgaag gtactcgcct tccaccggat
     2101 cgggtcaccg gaagaattga tcgacggatt caaggggata cgggagccgg cgaccgacgc
     2161 accgcactgc ccggtagccg atatcgatct cttcctcgtt cccggggtcg ccttctcgcg
     2221 gaacggggag cgcgtcggtt acggcggcgg ctactacgac gccaccctgc gggaacgggg
     2281 gccgcgatcg accgcgatcg gcgtaggctt cgacctgcag gtggtcgaac ccggtttcgc
     2341 cgacccgtgg gacgcacgac tggacggact cgtcaccgag tcatcggtga tgttcatcaa
     2401 tataacggaa aaggagtaac tcatggacac tac
//
LOCUS       DAOQUE010000433         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_52968_length_2433_cov_3.362069, whole genome shotgun sequence.
ACCESSION   DAOQUE010000433 DAOQUE010000000
VERSION     DAOQUE010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52968_length_2433_cov_3.362069"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PKH10_11450"
     CDS             <1..838
                     /locus_tag="PKH10_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNT28777.1"
                     /translation="IVFAHQSFESAREWVAALEPEDRPLLVFNAHTHDEYVRDAEGIY
                     FVQALKYMEKYARVEILRRKGKFSVVDARIVTLRDEASAVVPGGSKVRETVAGYLVKM
                     DRLAGATIMNAESDLAADRFMRLYACAVLDDFPEADVALSNPDAFRDKIRQGPIRMSD
                     IISMMPFENRLVISTISGKDLVYNLNLSENAVCGMEKVSDRWFKNGAPIDPSQSYRAV
                     IHEYIYAGGDYYKFLGPGITNQMTSKIWRSPVIRYLGEQGKNGVALETAYTELMKKYG
                     YK"
     gene            complement(845..>2433)
                     /locus_tag="PKH10_11455"
     CDS             complement(845..>2433)
                     /locus_tag="PKH10_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT28778.1"
                     /translation="IRALGIAVIGKEEHSVITDWTPDMALEVFSQHLGIALAAAPKIP
                     ALKPVMRPPSICPGCPYRAFALAAQKLKKQKKLYASFGEIGCSTLLHFFNAVDTILCM
                     GGSDGMRQGFVLSRPDHGAKVISVIGDSCECHSGLDGTRNAVFRNVPGVKILLDNRVT
                     AMTGGQPAPTSPNNLAGVPNKFSLRDAVAAEKCRMVACDSYDLKEVERLLVEALELAE
                     KGEFTCLILEGACIQEVAAEKKRRRLTIDRDKCKQCGLCGVCPGIEHDENKRPNFTTL
                     CTNCGSSSQICRQRCPFGAIVEESDEKKGRIAPPDLPKARDIATVTVDKAKLPASLRV
                     AIRGIGGQGNLFFGKVLSEVALRTPYAEAGIVKGDTHGMAQMGGPVISTFACGEVHSP
                     ILAPGSADVLVVMEMSEVLRPGFLDLLKPGGTVVLNRFAAVPVAVKKEDYPALDAIKK
                     ALEGYDLIELDALAAARGYGDTAGRTANVVVLGLLSTIAPFNAIPEEIWLSALMAVSP
                     ADAMRSANQIAFAAGRGLGK"
BASE COUNT          455 a          727 c          788 g          463 t
ORIGIN      
        1 catcgttttc gcgcatcagt cgttcgaaag cgcccgcgag tgggtcgcgg cgcttgagcc
       61 ggaggaccgg ccgcttctcg tcttcaacgc ccacacgcac gatgagtatg tgcgggacgc
      121 cgaggggata tatttcgtgc aggcattgaa gtatatggaa aaatatgcac gggtcgagat
      181 actgcgcagg aaagggaagt tctcggtcgt cgacgcccgc atcgtgacgc tacgcgacga
      241 ggcgtcagcg gtcgtcccgg ggggatcgaa ggtccgcgaa acggtggcgg ggtacctcgt
      301 caagatggat cggctcgccg gcgccaccat catgaacgcc gaaagcgatc tggccgccga
      361 tcgtttcatg cggctgtacg cctgcgcggt gctcgacgat ttccccgaag ccgatgtggc
      421 gctctcgaac cccgacgcgt tccgtgacaa gatccggcag gggcctatcc ggatgagtga
      481 catcatctcg atgatgccgt tcgagaaccg gctcgtcatc tcgacgatat cggggaagga
      541 tctcgtgtat aacctcaatc tgtctgagaa cgccgtctgc ggcatggaga aggtgagcga
      601 ccgctggttc aagaacggtg cgccgatcga tccgtcgcag agttatcgcg cggtcatcca
      661 tgagtatatc tacgccggcg gcgactacta taaattcctc ggtcccggca tcaccaacca
      721 gatgacctcg aagatatggc ggagccccgt catccggtat ctgggggagc aagggaagaa
      781 cggcgtggcg ctcgaaacgg cctacaccga actcatgaaa aagtacgggt acaagtagcg
      841 gtcgctactt tccgagtccc cggcccgccg cgaaggcgat ctggttggcc gacctcatcg
      901 catcggcggg cgacaccgcc atgagcgccg aaagccagat ctcttcgggt atcgcgttga
      961 agggcgctat ggtcgaaaga aggccgagca ccacgacatt agcggtgcgg cccgccgtgt
     1021 caccgtaccc gcgcgccgcc gcaagggcgt cgagttcgat gagatcgtat ccttcgagcg
     1081 cttttttgat ggcatcgagc gccgggtagt cctccttttt caccgcgacc ggcaccgctg
     1141 cgaaacggtt gagcaccacc gtgccacccg gctttagaag atcgaggaac ccggggcgga
     1201 gcacctcgct catctccatc accaccagca catcggcgct gccgggggcg agtatcggcg
     1261 aatggacctc gccgcacgcg aaggtcgaga tgaccggacc gcccatctgc gccatgccgt
     1321 gggtgtcgcc cttgacgatg cccgcctcgg cgtagggggt tcgcaacgcg acctccgaga
     1381 gcaccttgcc gaagaagagg ttcccctgcc cgccgatgcc gcgtatcgcg acccgcaggg
     1441 aggcggggag tttcgcctta tcgaccgtca ccgtggcgat atccctcgcc ttgggcaggt
     1501 cgggcggcgc gatcctgcct ttcttctcgt ccgactcttc gacgatcgcg ccgaacggac
     1561 agcgctggcg gcatatctgg gaactcgaac cgcagttggt gcagagcgtc gtgaagttgg
     1621 gccgtttgtt ctcatcgtgt tcgataccgg gacagacgcc gcacagaccg cactgtttgc
     1681 atttgtcgcg gtcgatggtc aggcgacggc gcttcttttc ggccgccacc tcctgtatgc
     1741 aggccccctc gaggatgaga caggtgaact cacccttttc ggccagttcc agcgcttcga
     1801 ccagcagtcg ttcgacctct ttgagatcgt aggaatcgca ggcgaccatg cggcatttct
     1861 cggcggcgac cgcgtcgcgc aggctgaatt tgttgggtac gccggcgaga ttgttgggcg
     1921 aggtgggggc cggttgaccg cccgtcatcg cggtgacgcg gttatcgagc aggatcttca
     1981 cgccggggac gttgcggaac acggcgttgc gtgttccgtc gagtcccgaa tggcattcgc
     2041 acgaatcgcc gatgacgctg atgacctttg cgccgtggtc ggggcgcgac agtacgaagc
     2101 cctgtcgcat gccgtcggag ccgcccatgc agaggatggt gtcgaccgcg ttgaaaaaat
     2161 ggaggagcgt cgaacagccg atctcgccga aggaggcgta aagttttttc tgctttttga
     2221 gtttctgggc cgcgagcgca aaggctcggt agggacagcc gggacagatc gacggcggtc
     2281 gcatgaccgg cttcaaagca gggatcttcg gcgcggcggc aagggcgata ccgagatgtt
     2341 gggagaacac ctcaagcgcc atatcagggg tccagtcggt gatgacggag tgctcctcct
     2401 tgccgatgac cgcgatgccg agtgcccgta tct
//
LOCUS       DAOQUE010000434         2431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_53029_length_2431_cov_4.695707, whole genome shotgun sequence.
ACCESSION   DAOQUE010000434 DAOQUE010000000
VERSION     DAOQUE010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53029_length_2431_cov_4.695707"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PKH10_11460"
     CDS             complement(<1..908)
                     /locus_tag="PKH10_11460"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNT28779.1"
                     /translation="MTCIKKNLLFLFVSWFILLSCTPVTESDPIATGDEEMGESDITD
                     EKDIQDEEVGEAEVVDDDTVATDELVVDETIGDETPDIDEPTIEFDEPDAPQTPIAYL
                     VTVEALRHTFEELAIIHSLTGIPTAVTTLEDICGGACDDGDPRNDTAAAIKAWATTRP
                     GIQHLILAGDIELVPSRAVHDKYSNVVAGTYEEDLKSDHYYADLSEWDGNHNGIYAED
                     PGDAPDLLPELAVSRIPVSDHDEAARYVAKARRYLTAYNSSDMQKALLIANVATNYNG
                     IDINAGYYFENEGRTVDAFPATFTLRR"
     gene            1046..2353
                     /locus_tag="PKH10_11465"
     CDS             1046..2353
                     /locus_tag="PKH10_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNT28780.1"
                     /translation="MENILDPLPSGPAVHFEIVVVILSLFFSAFFSATETALTALSDT
                     KVHNLLEKHPYAAGLLKKWLVKPSLLLSTILIGNNLVNILASVLAARIAHHYIGSFAD
                     AAAVGLMTLIVLIFGEITPKTYAKLNPEKVAVPALAFLRVFDLIFWPAAVILSRFSRL
                     LVRLMGGKDLGQEGLSITQHDIEYMIRKGSEHGVFEKEEQGELLSSVVEFRETLVGEI
                     MIPRTDVHFLETTDSIGEALTKISEWGHSRIPVYEENIDNVRGILLSKSLVAEFAKGG
                     VDLSRPIAPYLRRPVLFAPESQRISDTLKAMQSRRIHMAVVVDEFGGTSGIITLEDIL
                     EELVGDIKDEVDKDAHPIIKLKDDVISVDAHMSIYDLEEELAIKLPDDGEYNSLGGFI
                     TAHAGSVPAKGYKFEYQGVVFRVAESDERHIARVEIRRIPHRS"
BASE COUNT          484 a          701 c          710 g          536 t
ORIGIN      
        1 cgtcgcaggg tgaaggtggc cggaaaggcg tcgaccgtcc ggccctcgtt ctcgaaatag
       61 tagccggcgt tgatgtcgat gccgttgtag ttggtcgcga cgttcgcaat gagaagcgcc
      121 ttctgcatat cgctgctgtt gtaggcggtg agatatctcc gcgccttggc gacatagcgg
      181 gccgcttcgt catggtccga gaccgggatg cgcgataccg ccagttcggg gagcaggtcg
      241 ggcgcgtcgc ccggatcttc ggcgtagatc ccgttgtggt tgccgtccca ttcggaaagg
      301 tcggcgtagt aatgatcgct cttgaggtct tcctcgtaag tcccggcgac gacattggag
      361 tatttatcat ggacggcgcg cgagggcacc agttcaatgt cgccggcgag gatgaggtgt
      421 tggatccccg ggcgggtcgt cgcccatgcc tttatcgccg ccgcggtgtc gttgcgcggg
      481 tcaccgtcgt cgcaggcgcc tccacagata tcttcgagcg tcgtcacggc ggtcgggatg
      541 ccggtgaggg aatgtatgat cgcaagttct tcaaaggtgt ggcgtaacgc ctcgacggtc
      601 acgaggtagg cgatcggcgt ttggggtgcg tcgggttcgt cgaactcgat cgtcggttcg
      661 tcgatgtcgg gcgtttcgtc gccgatggtc tcatcgacga caagttcgtc ggtcgccacc
      721 gtatcatcat caactacttc ggcctctccc acctcctcgt cctgtatgtc cttttcgtcc
      781 gttatgtcgc tttcgcccat ttcctcgtcg cccgtcgcga tggggtcact ctccgtgacc
      841 ggtgtgcagg agagaaggat gaaccacgat acaaagagaa aaaggaggtt ttttttgatg
      901 caggtcacag tcagcctccg aaagagaacc tcttctttgc tcagaataac agccccaagg
      961 ccgtttggca ataaatagtt gataaatgcg gagaatctga tacaacacaa gggtttcggg
     1021 ttccttggcc ctttatgaac agaacatgga gaacatcttg gaccctctcc cttcaggccc
     1081 agccgttcat ttcgagatcg tcgtcgtcat cctttccctt ttcttcagcg cctttttcag
     1141 tgccaccgaa acggcactga ccgcgctttc cgacacgaag gtgcacaacc tgctcgaaaa
     1201 gcacccgtat gccgcggggt tgctgaaaaa atggctcgtc aagccgagtc tgctgctctc
     1261 gaccatactg ataggtaaca atctcgttaa catcctcgcc tcggtcctcg ccgcccgcat
     1321 cgcccaccac tacatcggca gtttcgccga tgcggcggcg gtcgggttga tgacgctcat
     1381 cgtcctgata ttcggcgaga taaccccgaa gacctacgcg aaactgaacc cggagaaggt
     1441 agcggtgccg gcactggcgt tcctacgggt gttcgacctg atcttctggc ccgccgccgt
     1501 gatcctttcg agattctcgc gtcttcttgt ccgcctcatg ggagggaaag acctggggca
     1561 ggaggggctc agcatcaccc agcacgatat cgagtatatg atacggaagg ggagcgagca
     1621 cggcgttttc gagaaagaag agcagggcga actcctctca tcggttgtgg aattccgcga
     1681 aacgctcgtc ggagagatca tgataccgcg caccgatgtc cacttccttg aaacgaccga
     1741 ctccataggg gaggcgctca cgaagatatc ggagtggggc cattcccgta tcccggtcta
     1801 tgaggagaac atagacaatg tgcggggcat cctgctttcc aagagtctcg tagccgaatt
     1861 cgccaaggga ggcgtcgatc tgagccgccc gatagcgccc tatctgcgcc ggccggtcct
     1921 gttcgcgccc gagagccagc ggatcagcga tacactcaag gcgatgcagt cccggcgcat
     1981 ccacatggcc gtcgtcgttg acgaattcgg cggcacttcc ggcatcatca cgcttgagga
     2041 catccttgag gagctggtcg gcgacatcaa ggacgaggtc gataaggatg cccaccccat
     2101 catcaaactc aaagacgatg tcatcagcgt ggacgcccat atgtcgatat acgacctcga
     2161 agaggaactg gcgataaaac tgcccgatga cggtgaatac aattcgctcg gcggtttcat
     2221 caccgcccac gccggatcgg tgccggcgaa aggttacaag ttcgaatatc agggggttgt
     2281 gttccgcgtc gccgaaagcg atgagcggca catcgcccgt gttgagatac gcaggatccc
     2341 gcaccgctcc tgatcgtcgc gacagcggtt ttcaacaccg ctgttgataa ccccttccgt
     2401 ttcaaggcct aactccttga tgcgccgcgt t
//
LOCUS       DAOQUE010000435         2424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_53274_length_2424_cov_4.875897, whole genome shotgun sequence.
ACCESSION   DAOQUE010000435 DAOQUE010000000
VERSION     DAOQUE010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53274_length_2424_cov_4.875897"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PKH10_11470"
     CDS             complement(<1..511)
                     /locus_tag="PKH10_11470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT28781.1"
                     /translation="MQIFGPVPSRRLGWSLGVNTLPEKICSYSCAYCQVGLTEHFDRE
                     RRRYADPQEVARAVAEKIAKVVAKGGTVDVVSFVPDGEPTLDAELGAQIAAVRSMGKV
                     AVITNGSLMDRADVRRDLLQADIVSVKIDTVDPTAWRKIDCPTTGLELPALLDGIRLF
                     AEEFTGTLLT"
     gene            complement(528..944)
                     /locus_tag="PKH10_11475"
     CDS             complement(528..944)
                     /locus_tag="PKH10_11475"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNT28782.1"
                     /translation="MTEMVSGLTAVQEDYLEAIYRLTVERKVARNKEIAEALGVKTAT
                     VTGAVQHLAELGYVNYESHGYVTLTAIGAKLARELLERHELFYEFFHEILGLDEKVAD
                     EVACRTEHHITGEPYRRFRKLVAHFQKCPHPEKYQN"
     gene            1108..2358
                     /locus_tag="PKH10_11480"
     CDS             1108..2358
                     /locus_tag="PKH10_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNT28783.1"
                     /translation="MFMMFWEHGASAGARISGRVDRWALHPRWGLLLFGSGVYLFLQL
                     VFAIGGPAALLLEHLFAHFSTLVADAWPFGADSFSLAIVTSAIAGVGGVLAYTPNLFI
                     LFIGMAFFEQSGYLARGAHLLDRFMARIGLSGGCFLPLALGFGCSVPAVMATRLIKDR
                     RERLTAIMMVPFLSCSGRLPVYLLIVSAFFPFAWQAAAMLGVYLVGVLTSILVALFLR
                     RTVLPGDRCGCAVELPPYALPPMRDLLRSTWERGRHFLEKAGTTILIASILLGMLSAW
                     PRTGGSPEEQARGSLLGRIGAATEPAFALMGGDWRVADAALASLAAKEMFVSQSAILF
                     RQEGTGIIGSGLSEKLRDTYPPAAALAFLVFILFSAPCIATFATVKAETGSWKWATAQ
                     YAGMTLLAYIAAVIVYQAARLIGG"
BASE COUNT          366 a          741 c          782 g          535 t
ORIGIN      
        1 tggtgagtag ggtccccgtg aactcctccg cgaagaggcg gatgccgtcg aggagcgcgg
       61 gaagttcgag cccggtggtc gggcagtcga tcttccgcca agcggtcggg tcgacggtgt
      121 ctatcttgac cgatacgatg tcggcctgca aaaggtcgcg ccgaacatcg gcgcgatcca
      181 tgagcgagcc gttggtgatg acggcgacct tgcccatcga ccggaccgcc gcgatctgcg
      241 cgccgagctc cgcatcgagc gtcggttcgc cgtcggggac gaacgagacg acatcgaccg
      301 ttcccccttt ggcgacgacc ttcgcgatct tttcggcgac cgcccgcgcg acctcctgcg
      361 ggtcggcgta cctgcgacgc tctcggtcga aatgctcggt cagtccgacc tgacagtaag
      421 cgcaggagta ggagcatatc ttctcgggca gggtgttgac gccgaggctc catccgagtc
      481 ggcgtgaggg gacgggaccg aatatctgca tgtggtgttc gctccgctca gttctggtat
      541 ttttccggat gcgggcattt ctggaaatgg gcgacgagtt tgcggaagcg gcggtagggt
      601 tcgccggtga tgtggtgttc ggtccggcag gcgacctcgt cggccacctt ttcgtcgagg
      661 ccgaggatct cgtggaagaa ctcatagaag agttcgtgcc gttcgagcag ttctctcgcc
      721 agtttcgctc cgatggcggt cagcgtcaca tagccgtgac tctcgtagtt cacatagccg
      781 agttcggcga ggtgctggac cgcgccggtc accgtggcgg tcttgacgcc gagcgcctcc
      841 gcgatctcct tgttgcgcgc caccttccgt tcgaccgtca ggcggtagat cgcctcgaga
      901 taatcctcct gcaccgccgt aagtcctgaa accatttcgg tcatgcgcac ctcccctagc
      961 aggttgacct tgcatgagta taatgtccaa agcgctcgtg tcaacaacaa aaattaggaa
     1021 agacgaaaaa agttcttgac aagttagggc ttgctaacta tattcaaaaa ggcgaaaaga
     1081 gttctctgat gcgagggcca tcttgcgatg ttcatgatgt tctgggagca tggggcaagc
     1141 gccggggcgc ggatatcggg tcgcgtcgac cgttgggcgc tccacccgcg gtgggggctc
     1201 ctgctgttcg ggtccggggt ctaccttttc ttgcagttgg tctttgcgat cggcggcccg
     1261 gccgccctct tgctcgaaca tctcttcgcc cacttctcga cccttgtggc cgatgcgtgg
     1321 ccgttcggcg ccgattcgtt ctcgctcgcg atcgtgacga gcgccatcgc gggggtcggc
     1381 ggtgtgctcg cctatacgcc caatctcttt attcttttca tcggcatggc ttttttcgag
     1441 cagagcggct atctggcgcg tggcgcccac ctgcttgacc ggttcatggc tcgcatcggc
     1501 ctgtcgggtg gttgcttttt gccgctcgcg ctcgggttcg gctgttcggt cccggccgtc
     1561 atggcgacgc gactcatcaa ggaccggcgt gaacggctga ccgccatcat gatggtgccg
     1621 ttcctcagtt gttccggtcg tctgccggta tatctgctca tcgtttcggc gttctttccg
     1681 ttcgcgtggc aggcggcggc gatgctcggc gtctatctcg tcggcgtact cacctcgata
     1741 ctcgtcgccc tctttctgcg gcgcaccgtg ctcccgggcg accggtgcgg gtgcgcggtc
     1801 gaactacccc cctatgccct gccgccgatg cgcgatcttt tgcggtcgac ctgggaacgg
     1861 ggccgtcact tcctcgaaaa ggcggggacc acgatactta tcgcgagcat cctgctcggc
     1921 atgctctcgg cgtggccgcg gaccggcggg tcgcccgagg aacaggcgcg tgggtcgctg
     1981 ctcggtcgca tcggcgcggc caccgaaccg gccttcgcgt tgatgggcgg cgattggcgt
     2041 gtcgccgacg ccgcgctcgc gtcgcttgcc gccaaggaga tgttcgtgtc acagtcggcg
     2101 atactgttcc ggcaggaggg gaccggcatc atcgggtcgg ggctttcgga aaaactgcgc
     2161 gacacctatc cgcccgcggc ggcgctcgcc tttctcgtgt tcattctctt ttcggctccc
     2221 tgcatagcca ccttcgcgac ggtcaaggcc gagaccggca gttggaaatg ggcgacggcg
     2281 cagtatgcgg ggatgacgct ccttgcctat atcgcggcgg tcatcgtcta tcaggcggcc
     2341 cgcctcatcg gggggtgaag ggatagctac gcgttcttac tgaaacggtc catgatgacg
     2401 gcgcaactgg cgtcaccggt caca
//
LOCUS       DAOQUE010000436         2422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_53348_length_2422_cov_4.253908, whole genome shotgun sequence.
ACCESSION   DAOQUE010000436 DAOQUE010000000
VERSION     DAOQUE010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53348_length_2422_cov_4.253908"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PKH10_11485"
     CDS             complement(<1..645)
                     /locus_tag="PKH10_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT28784.1"
                     /translation="MRFGTPHYLLILFLWLPALIVYLAVARRRRDRFAGSFSPGHRDL
                     LVAVSPNALWTRRLFLCMALAFLTFAAARPQMGETEIHLSGQGADMAVAFDVSLSMLA
                     EDEDGPRFEKGARLLADAVAELGGDRVAIVPFAGSAFLQLPLTADHNTALAVTSALRP
                     GMIERPGSAIGAAVSLALETLKSGPEESDKLLVIVSDGEDPALDFEQVRRELEEA"
     gene            complement(642..1628)
                     /locus_tag="PKH10_11490"
     CDS             complement(642..1628)
                     /locus_tag="PKH10_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT28785.1"
                     /translation="MTFLSPLHLFALLLLPVLWLMLRGRSATPASLLFSDHAGLRSVA
                     DTRWRICRVFDLIAFILLVVALARPVALDRVVTPPVEGKDIVIALDVSGSMEALDFQP
                     KNRIESAKGVIERFVQGRRSDRLGLVFFAKDSFLQVPLTTDYTMFVNLLRRLETGVID
                     DGTAIGNGLGLALSRLEDSKAKSRLVILLTDGDNNSGNISPETAAEAAKTLGIKVYTI
                     LIGTDKPVPFPAGKDIFGRQTFRTVPMKINPELLKKIADTTGGHAYRSLSTDELDRAF
                     AEIDKLEKSPIPAQKYRLYDEFAPYFILAAILAILLGRLFALLFPLYPEVER"
     gene            complement(1688..>2422)
                     /locus_tag="PKH10_11495"
     CDS             complement(1688..>2422)
                     /locus_tag="PKH10_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HNT28786.1"
                     /translation="DLLTGKVKTKVQDAYSMRSTPQVIGAAHDAIRWARSQVETELNG
                     VGDNPIFIPEEKLTLSGANFQGTPVSLPMEMVGQAITMVSVLSERRMNRLNNPALSVG
                     LPPFLTKNPGMFSGLMLSQYTADTLIVEQRILSAPAATQSIPAAADQEDFVSMGMNTA
                     LKNLQILENGNGVLGIEFMAAAQALDIRGFKTGDGVAAAHKVIREKVAFLEIDRPLYP
                     DHTAMQELVRTGAILDAVEAAVGSLG"
BASE COUNT          448 a          725 c          824 g          425 t
ORIGIN      
        1 tgcctcttcc agttctcggc gtacctgttc gaagtcaagc gccgggtcct cgccatcgga
       61 aacgatgacc agtagtttgt cgctctcctc cggaccgctt ttaagcgtct caagcgcgag
      121 cgataccgcc gcgccgatcg cggaaccggg gcgctcgatc ataccgggcc gcagggcgga
      181 ggtgacggcg agagcggtgt tatggtcggc ggtgagcggc aactgcagaa aggccgaccc
      241 ggcgaacggg acgatggcga cgcgatcacc tcccaattcc gctactgcat cggcgagcag
      301 gcgcgccccc ttctcgaacc gggggccatc ctcgtcctcg gcgagcatcg aaagactcac
      361 atcgaaggcg accgccatgt cggccccctg tcccgaaaga tgtatctcgg tctcgcccat
      421 ctggggccgg gcggcggcga aggtaaggaa ggcgagcgcc atgcacagga acaaccgtcg
      481 cgtccagagc gcattcggcg aaacggcgac gagtaggtcg cgatgaccgg gggagaaaga
      541 gcccgcgaac cggtcgcgcc ggcggcgcgc gacggcgaga tagacgatga gcgccggcag
      601 ccaaaggaac aggataagca ggtagtgcgg cgtgccgaac ctcatcgttc gacctccggg
      661 tagagcggga acaggagcgc gaagagccgc ccgaggagta tcgcaagtat cgccgcgagt
      721 atgaagtagg gggcgaactc gtcgtaaagc cggtacttct gggccggtat cgggcttttt
      781 tccagtttgt ctatctcggc gaaggcgcgg tcgagttcgt cggtcgacag ggagcggtag
      841 gcgtggccac cggtcgtgtc ggcgatcttt ttgagcagtt cggggtttat cttcatcggg
      901 acggtgcgga aggtctgtcg gccgaagatg tccttcccgg cggggaaggg gaccggcttg
      961 tcggtgccga tgagtatcgt gtagaccttg atgcccagtg tcttcgccgc ttcggctgcc
     1021 gtttcggggc tgatattgcc gctgttgttg tcgccgtcgg tgagcaggat cacgagccgt
     1081 gattttgctt tcgaatcctc gagccgcgac agcgcgagcc cgagcccgtt gccgatggcg
     1141 gtgccgtcat ctatcacgcc ggtctcgagg cggcgcagga gattgacgaa catcgtatag
     1201 tcggtcgtga gcggtacctg caggaacgag tccttggcga agaacacgag tccgagccgg
     1261 tcgctccggc gtccctgcac gaaccgttcg atcaccccct tcgccgactc gatgcgattc
     1321 ttgggttgga aatcgagcgc ctccatcgac cccgacacat caagggcgat gacgatatcc
     1381 ttgccctcga ccggcggggt gacgacccgg tcgagcgcga cgggccgggc aagtgcgacg
     1441 acgaggagga taaaggcgat cagatcgaat acccggcaga tgcgccaccg tgtgtcggcg
     1501 acgctccgca ggccggcatg gtcggaaaag aggagcgatg ccggcgtcgc cgatctgccg
     1561 cggagcataa gccacaggac cggcaggaga agcagggcga ataggtgtag tggcgaaaga
     1621 aacgtcatgc ggcgacccta caaaaaaaca ccgctaataa cagagggggc aggcccttta
     1681 ttccggctca gccgagactc ccgaccgccg cttcgaccgc gtcgagtatc gcgccggtgc
     1741 ggaccagttc ctgcatcgcg gtgtggtcgg ggtagagcgg gcggtcgatc tcgaggaatg
     1801 cgaccttttc gcggatgacc ttgtgggccg ccgcgacgcc gtcgcccgtc ttgaaaccgc
     1861 ggatatcgag cgcctgcgcc gccgccatga actcgatgcc gagcaccccg ttgccgttct
     1921 cgagtatctg gaggttcttg agggcggtat tcatccccat cgaaacgaaa tcctcctgat
     1981 cggccgccgc cgggatcgac tgggtcgcgg cgggagccga caggatgcgc tgttcgacga
     2041 tgagggtatc ggcggtgtac tgcgacagca tcagccccga gaacatgccg gggttcttcg
     2101 tgaggaaggg cggcaggccg accgacagcg ccgggttatt gagccggttc atccgccgct
     2161 ccgaaaggac gcttaccatc gtgatcgcct gaccgaccat ctccatcggc agcgacaccg
     2221 gtgtcccctg gaagttggcg cccgaaaggg tcagtttctc ctcggggatg aagatcggat
     2281 tgtcgcccac gccgttcagt tcggtctcga cctgcgatcg ggcccagcgg atggcgtcat
     2341 gcgccgcgcc gatgacctgc ggggtggagc gcatcgaata ggcgtcctgc accttggtct
     2401 tcaccttgcc ggtgaggagg tc
//
LOCUS       DAOQUE010000437         2414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_53650_length_2414_cov_3.505723, whole genome shotgun sequence.
ACCESSION   DAOQUE010000437 DAOQUE010000000
VERSION     DAOQUE010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53650_length_2414_cov_3.505723"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PKH10_11500"
     CDS             complement(<1..839)
                     /locus_tag="PKH10_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNT28787.1"
                     /translation="MKNIALLCTLALLLVATPLFSEEPPLNTKSYTPSPFTHGILSTH
                     GGEIMDFLEMNIGLSFMYSYKPLVLEFTDTTGTTTRNILEHKFEADLAFAVGLWKYID
                     IAMNLPINLYHGGEGLYEAKMHAAGVGDMAFFPRVGYTFEKDKVSIAFIPEVTIPTGR
                     QVDRLMGYATATFVPTFAVSAVLGDFGIATDLFYRLMDKRELADLTAGDEIGLKLAGR
                     YTPIDPLDIEAEIIARTVATDFFSASNAIFVDGNVGVQYRFPQVPGLMLHGGAGWGMT
                     QGYG"
     gene            complement(852..>2414)
                     /locus_tag="PKH10_11505"
     CDS             complement(852..>2414)
                     /locus_tag="PKH10_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNT28788.1"
                     /translation="CPLIANIQTDTDADGIGDACDDDDDNDGLTDSEEEALGTDPLDT
                     DSDNDGIDDATEVGDDTGDPDNTDGDDHIDALDTDSDNDGIGDANEGAGDTDGDGTPD
                     YRDGDSDGDGIDDGDEGAGDTDGDGTPDYLDDGTDNCRLIPNTGQEDSDLNGVGDACD
                     GDLDGDGTPNGTDNCPTLPNADQKDTDLDGTGDVCDTDDDGDGLTDTEEGAIETDPLK
                     VDTDNDGIDDATEVGDDPNDPLDTDEDGTIDALDDDSDGDGIDDAVEGSGDSDDDGTP
                     DYLDPDSDDDGIDDGTEGNGDSDDDGIPDYLDADSDDDGVADGIDNCRIVGNAGQEDT
                     DGNGVGDACDGDLDGDGVLNDDDNCPNIANPWQEDADSDGIGDACDTPNDDDAITDDG
                     VVLPDEDTVIEPDDFIVSPDEDAVVEPDNDTVIADEILADEDSVLSDDIVADEDMVDE
                     DMTEVEPADDTTAIPDEDMTEAEPVDDTTVITDEAVDEDTLTVDDGVPVPDDPYGTGV
                     TIRGGCGCSVVF"
BASE COUNT          358 a          724 c          794 g          538 t
ORIGIN      
        1 ccgtagccct gcgtcatccc ccagccggcg ccaccatgca gcatgagccc gggaacctgc
       61 gggaaacggt actggacacc cacattacca tcgacgaata tagcgttgga tgcagagaag
      121 aagtcggtcg ctacggttcg cgcgattatc tccgcctcga tgtcgagcgg gtctatcggg
      181 gtgtagcgtc ccgccagttt cagccctatc tcgtcgcccg cggtaagatc ggccagttcg
      241 cgtttgtcca tcagccggta gaagaggtcg gtcgcgatgc cgaaatcgcc cagcaccgcc
      301 gacactgcga aggtcgggac gaaggtggcg gtggcgtagc ccatgagccg gtcgacctgc
      361 cgtcccgtcg ggatggtcac ctcggggatg aatgcgatgc tcaccttgtc cttctcgaag
      421 gtgtagccga cgcgcgggaa gaaggccatg tcgccgacgc cggcggcgtg catcttcgct
      481 tcgtagagtc cctcgccgcc gtggtaaaga ttgatcggca ggttcatcgc gatgtcgatg
      541 tacttccaca ggccgacggc gaaggcgagg tcggcctcga atttatgttc cagtatgttc
      601 cgggtggtcg taccggtggt gtcggtgaat tcgagcacca gcggtttata gctgtacatg
      661 aacgagaggc cgatattcat ttcgaggaag tccatgatct cgccaccgtg ggtcgaaagg
      721 atgccgtggg tgaaggggga gggggtgtag gatttcgtgt tgagcggtgg ttcctccgaa
      781 aaaagcggag tggcgacaag gagcagggcc agcgtgcaga ggagagcgat gttcttcatg
      841 gcattccccc cttaaaacac gacgctgcag ccgcaaccgc cgcggatggt gacacccgtg
      901 ccgtacgggt cgtcgggcac cgggacaccg tcatcgacgg taagggtgtc ttcgtctacc
      961 gcttcgtcgg tgatgacggt ggtgtcgtcg accggttcgg cctcggtcat gtcttcgtcg
     1021 gggatggcag tggtgtcgtc ggccggttcg acctcggtca tatcctcatc gaccatatct
     1081 tcatcggcga caatatcgtc ggacagcacg gaatcctcgt cggcgaggat ctcatcggcg
     1141 atcacggtgt cgttgtccgg ttcgaccacg gcatcctcgt ccggcgagac gatgaaatca
     1201 tccggctcga tcaccgtgtc ctcatcgggc aggaccacgc catcgtcggt gatcgcatcg
     1261 tcgtcgttcg gggtgtcgca ggcatcgccg atgccgtccg agtcggcgtc ctcctgccag
     1321 gggttggcga tgttcgggca gttgtcgtcg tcgttcagca cgccgtcgcc gtcgaggtcg
     1381 ccgtcgcacg catcgccgac gccgttcccg tcggtgtcct cctgtccagc gttccccacg
     1441 atgcggcaat tgtcgatgcc gtcggcaaca ccatcgtcat ctgagtcggc gtcgagatag
     1501 tcgggtattc cgtcatcgtc gctgtcgccg ttgccttcgg tgccgtcgtc gatgccgtcg
     1561 tcgtcactgt cgggatcgag ataatcgggc gttccgtcgt cgtcgctatc gccggaacct
     1621 tcgacggcgt catcgatgcc gtcgccgtct gaatcgtcgt cgagcgcatc tatcgtgccg
     1681 tcctcatcgg tatcgagcgg gtcgttcgga tcgtcgccga cctcggtcgc gtcgtcgatg
     1741 ccgtcgttgt cggtatcgac cttgagcgga tcggtttcga tcgccccttc ttcggtgtcg
     1801 gtcagaccgt cgccgtcatc gtcggtatcg cagacatcgc cggtgccgtc gagatcggta
     1861 tccttctggt cggcgttcgg cagggtcggg cagttgtcgg tgccgttggg ggtgccgtcg
     1921 ccgtcgaggt cgccgtcgca ggcgtcgccg acgccgttga gatcgctgtc ctcctgaccg
     1981 gtgttcggga tcaaacggca gttgtcggtg ccgtcgtcga gatagtcggg ggtgccgtca
     2041 ccgtcggtgt cgccggcacc ttcatcgccg tcgtcgatgc cgtcgccgtc cgagtcgccg
     2101 tcgcggtagt cgggggtgcc gtcgccgtcg gtgtcgccgg caccttcatt ggcgtcaccg
     2161 atgccgtcgt tgtctgaatc ggtgtcgagc gcgtcgatgt gatcgtcgcc gtcggtatta
     2221 tcgggatcgc cggtatcgtc gccgacctcg gtcgcgtcat cgatgccgtc attgtcgctg
     2281 tcggtatcga gcggatcggt gccgagcgcc tcttcttcgc tgtcggtcag gccatcgttg
     2341 tcatcatcat cgtcacaggc atcgccgata ccgtcggcgt cggtgtcggt ctgtatgttg
     2401 gcgatgagcg gaca
//
LOCUS       DAOQUE010000438         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_53678_length_2413_cov_4.294741, whole genome shotgun sequence.
ACCESSION   DAOQUE010000438 DAOQUE010000000
VERSION     DAOQUE010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53678_length_2413_cov_4.294741"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="PKH10_11510"
     CDS             <1..1266
                     /locus_tag="PKH10_11510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HNT28789.1"
                     /translation="VRTCEEIQGVSALDFSGRGSKSKISTAFDRSLNLSTCVACGQCV
                     MACPTGALTERSSIREVMNALQDPKKTVVIQHAPAISVSLAEEFGLPAGSDIMGFMTA
                     ALKKIGFKAVFDTSFSADLTIMEEATELVERVKGGGVLPMITSCSPAWIKFAETFYPG
                     VLPHLSTCKSPQQMLGAVIRHYWADMQGLDKKAVYSVSAMPCTAKKFEAEREEMLVKG
                     APDIDAVLTTRELAKLIRMYSIDLGKLEPVACDLPFGERSSAGKLFGASGGVMEAAIR
                     TAHYLITGLELENLVVAAVRGGEGLRETTLTLGGLTLKAAVVSGLGSARQVLDEVAAG
                     KSRYHFIEIMSCPGGCINGGGQPRGLDRENVRKRLAALYAIDAAATLRVSHKNGAVAD
                     LYEKFLGKPGGHTSHELLHTTYQARTDLL"
     gene            1278..1673
                     /locus_tag="PKH10_11515"
     CDS             1278..1673
                     /locus_tag="PKH10_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT28790.1"
                     /translation="MNEEKRKILLVDDDPDFLEPVRMLLTAHGYTVEAAHSEEGALKC
                     LETFKPDLAVLDLMMDHYDSGFIVGHKLKGRYPDAKVIIATGVTGETGYRFSADSQYG
                     ANWTGADLIMDKGIRPDQMLREVKRLLGA"
     gene            1676..>2413
                     /locus_tag="PKH10_11520"
     CDS             1676..>2413
                     /locus_tag="PKH10_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT28791.1"
                     /translation="MNTLRLLIVDDDPRMRESCSLVLDGLRITMPWLPDEYALKVLFA
                     GTAEEAKGIIEAGPVDLLLLDQRLPGMQGTDLLAYIVEKELDLQTVMITGNASLDTAL
                     NATRNGASEILAKPFTPEELRLSVTKAARFLILQRIAAKLDEERKKTRFEFISVLSHE
                     LKSPLAAVTGYLDMLRNRTLGDDLVAYDSCVERSLLRLEGMRRLIIDMLDLTRIESGI
                     KVRTMAVHNLADIAADMIDGVRAQAAKR"
BASE COUNT          485 a          786 c          775 g          367 t
ORIGIN      
        1 gtccgcacct gcgaagagat acagggggtc tcggcgctcg acttcagcgg ccgcggctcg
       61 aaaagcaaga tatcgaccgc cttcgaccgg agcctcaacc tctcgacctg cgtcgcctgc
      121 ggccagtgcg tcatggcctg cccgaccggc gccctcaccg aacgctccag catccgcgaa
      181 gtgatgaacg ccctgcagga cccgaaaaaa acggtggtca tccagcacgc cccggccatc
      241 agcgtgtcgc tcgccgagga gttcggactt cccgccggga gcgacatcat ggggttcatg
      301 accgcggcgt tgaagaaaat agggttcaaa gcggtgttcg acacctcctt ctcggccgac
      361 ctcaccatca tggaggaggc gaccgaactg gtggagcggg tgaagggggg cggcgtcctg
      421 ccgatgatca cctcctgcag tccggcgtgg ataaagttcg ccgagacctt ctaccccggc
      481 gtcctgccgc acctttcgac ctgcaagagc ccccagcaga tgctcggcgc ggtcatccgt
      541 cactactggg ccgatatgca ggggctcgat aaaaaggcgg tctattcggt cagcgcgatg
      601 ccctgcaccg ccaagaaatt cgaggcggag cgcgaagaga tgctggtgaa gggggccccc
      661 gacatcgacg cggtcctcac gacccgcgaa ctggccaaac tcatccggat gtactcgata
      721 gacctcggca aactcgaacc ggtcgcctgc gacctgccgt tcggcgagcg gtcgagcgcc
      781 gggaaactct tcggcgcgag cggtggggtg atggaggcag cgatccggac ggcccactac
      841 ctcatcaccg gcctcgaact ggagaacctc gtggtcgcgg cggtgcgcgg cggtgaaggt
      901 ctgcgcgaaa cgaccctcac gctcggcggg ctgaccctca aggcggcggt cgtgagcggc
      961 ctcgggagcg cgcggcaggt gctcgacgaa gtggcggcgg gcaagagccg ctaccacttc
     1021 atcgagatca tgtcctgtcc cggcgggtgt atcaacggcg gcggtcagcc gcgcggcctc
     1081 gaccgggaaa atgtgagaaa gcggctggcg gccctctacg cgatcgatgc ggcggcgacc
     1141 ctgcgggtga gccataagaa cggcgcggtg gccgatctct acgaaaagtt cctcggcaaa
     1201 ccgggcggac atacgagcca cgaactgctc catacgacct atcaggcgcg gaccgatctg
     1261 ttataaggga gacgacgatg aacgaagaga aacggaagat actgctggtc gacgacgacc
     1321 ccgatttcct cgaaccggtc aggatgctgc tgaccgccca cggctacacc gtggaggcgg
     1381 ctcacagcga agagggggcg ctgaaatgtc tcgagacctt caagcccgac ctcgcggtgc
     1441 tcgacctgat gatggaccac tacgacagcg gcttcatcgt cgggcacaag ctcaaggggc
     1501 gctaccccga cgccaaggtc atcatcgcga ccggcgtcac cggcgagacc ggctaccggt
     1561 tctcggccga ttcgcagtac ggcgcgaact ggaccggcgc cgacctcatc atggacaagg
     1621 ggatccggcc cgaccagatg ctgcgtgaag tgaaacggct gctgggagca tgaccatgaa
     1681 caccctgaga ctcctcatcg tcgacgacga tccgcggatg cgcgaaagct gttcgctggt
     1741 gctcgacggc ctgcggatca cgatgccgtg gctgcccgac gagtacgcgc tgaaggtcct
     1801 cttcgcgggg accgccgagg aggcgaaggg tatcatcgaa gcggggccgg tcgatctgct
     1861 gctgctcgac cagcggcttc ccggcatgca ggggaccgac ctactcgcct acatcgtcga
     1921 gaaggaactc gacctccaga cggtcatgat caccggcaac gcatcgctcg acacggcgct
     1981 caacgccacc cgcaacggcg cgagcgagat actcgccaag cccttcaccc ccgaagagtt
     2041 gcggctgagc gtcaccaagg cggcgcggtt cctgatactc cagcggatcg cggcgaaact
     2101 cgacgaggag cggaaaaaga cccggttcga gttcatcagc gtcctttcgc acgaactcaa
     2161 aagcccgctc gcggcggtga ccggctatct cgacatgctg cgcaaccgca cgctcggcga
     2221 cgatctggtc gcctacgact cctgcgtcga acggtcgctc ctgcggctcg aagggatgcg
     2281 gcggctcatc atcgacatgc tcgacctcac ccggatagag tcggggataa aggtgcgcac
     2341 catggccgtt cataacctcg ccgacatcgc cgccgacatg atcgacgggg tccgggcgca
     2401 ggcggccaaa cgc
//
LOCUS       DAOQUE010000439         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_53751_length_2411_cov_5.197368, whole genome shotgun sequence.
ACCESSION   DAOQUE010000439 DAOQUE010000000
VERSION     DAOQUE010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53751_length_2411_cov_5.197368"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKH10_11525"
     CDS             complement(<1..302)
                     /locus_tag="PKH10_11525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT28792.1"
                     /translation="MSHKIAVCIPTYNEKENILAIAQAVRGALPTADILVVDDSSPDG
                     TADIVRGFQQENPWMKLHVRKEKKGLGAAYIDGFQRLIEGGYDIIVQMDADFSHQP"
     gene            459..1217
                     /locus_tag="PKH10_11530"
     CDS             459..1217
                     /locus_tag="PKH10_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28793.1"
                     /translation="MNIERKDRLEMTGFLANVKIDGIYNTFPLVDINEEGIGIHVPVQ
                     LPVKEGDQFEIRYDIIDEKYGSFDTKVNYLIKKEGVAGTGIVRRILPVNDKLKLVGLK
                     MDYIADEAKEAKEKVKKAREIIKKLNDFPKSYIEFGFQVEEQARDPRLRGYPTFEGVI
                     ATENHMLKFLVDAEYEYLFLDNDYVSIKLPIRGPNGDFFVIQGHVKENHIFNMYKNLI
                     SVELDVWDKNDQELLKRELTRHNIESEDILRFPR"
     gene            complement(1260..1727)
                     /locus_tag="PKH10_11535"
     CDS             complement(1260..1727)
                     /locus_tag="PKH10_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNT28794.1"
                     /translation="MAISKQAALIKIIDVLGAQRFTTLATDTGDELHTSLVCYTFSKD
                     LHCLFFGTPRTTRKYKNLKKNGKNAFNVSDHRNEEDDTHRAFSVTAHGTAYELVDDRE
                     KYAALLIKRFPRLERFYRDPAVAIFRVKIDEYSITGDFQDVVTIGTSRDFCKE"
     gene            1868..>2411
                     /locus_tag="PKH10_11540"
     CDS             1868..>2411
                     /locus_tag="PKH10_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28795.1"
                     /translation="MEDRIMKNASVFLLLFFVASIFSSCAGSRVLGSDGDAVPDQDNG
                     GPEEGSVCAEENAMRCHGNEVMQCDGEIWVVIGDCADYGMECYEYGDEAVCTNNVPDG
                     DADEWPELDGDIDEYVPACGNAIIDTWEECDSSGVACTTIDSVTYSGGMADCLEDCTG
                     YDTSDCQMAIPPCSLDATFLD"
BASE COUNT          548 a          617 c          688 g          558 t
ORIGIN      
        1 ttgggctggt gcgagaaatc ggcatccatc tggacgatga tgtcgtaccc gccttcgatg
       61 agccgctgga acccgtcaat gtaggcggcg ccgagcccct ttttctcctt gcggacatgg
      121 agtttcatcc acggattttc ctgttggaag ccgcgcacga tatcggccgt gccgtcgggc
      181 gaactgtcgt ccaccacgag tatgtcggcc gtcggcaggg cgccccgcac cgcctgcgcg
      241 atggcgagga tgttctcctt ctcgttgtag gtcgggatgc ataccgcgat cttgtgggac
      301 atgggatcac ctctcgaaaa gaacggagaa gtatatacgg ggaaaaggcg ccgaagcaac
      361 atatttcatc gctggcggct ctttttttga cattttcgcc gcttgcgggt atcctctccg
      421 gcgtaggata ttgttttttc aacctatagg agggaacgat gaacatcgaa agaaaagacc
      481 gtctcgagat gaccgggttc ttggccaatg tcaagataga cggcatctac aacaccttcc
      541 cgctcgtgga catcaacgaa gagggcatcg gtatccatgt cccggtccag ttgccggtca
      601 aagagggcga ccagttcgag atccgctatg acatcatcga cgagaagtac ggtagtttcg
      661 acacgaaggt caattacctc ataaagaagg aaggcgtcgc cggcaccggt atcgtcaggc
      721 gcatcctgcc ggtcaatgat aaacttaaac tggtcggtct caagatggac tatatagccg
      781 acgaggcgaa agaggccaag gaaaaggtca aaaaggcgcg cgagataata aagaaactca
      841 acgacttccc gaaatcctat atagaattcg ggttccaggt cgaagagcag gcgcgcgatc
      901 cgcggctccg cggttatccg accttcgaag gggtcatcgc gaccgagaac cacatgctca
      961 agttcctcgt ggacgccgag tacgaatatc tcttcctcga taacgattat gtctccatca
     1021 aactgcccat caggggaccg aacggtgatt tctttgtcat tcagggtcat gtcaaagaga
     1081 accacatctt caatatgtac aaaaatctca tcagtgtcga actcgatgtg tgggacaaga
     1141 acgatcagga actcctgaaa cgcgaactga cccgccacaa tatcgaaagc gaggacatcc
     1201 tccgcttccc gcgctgatcg gtcgatcagg ctcgcgtcct caccggatag actttttctt
     1261 tattccttgc agaaatccct gctggtgccg atggtcacga catcctgaaa atcgccggtg
     1321 atgctgtact cgtcgatctt cacgcggaat atcgccacgg ccggatcgcg gtagaaccgt
     1381 tcgagacggg gaaagcgttt gatgaggagc gccgcatact tttcgcggtc atccaccagt
     1441 tcataggcgg tgccgtgggc cgtcaccgaa aatgcacggt gcgtgtcgtc ctcttcgttc
     1501 cggtggtccg atacattgaa ggcgttcttg ccgttcttct tgagattctt gtatttgcgg
     1561 gtggttcgcg gcgtcccgaa aaagaggcag tgaagatcct tcgaaaaggt gtagcagaca
     1621 agactcgtgt ggagttcgtc cccggtgtcg gtagcaagcg tcgtgaatct ctgggcgccg
     1681 agcacatcga ttatcttgat gagcgccgcc tgtttagata tagccatcgg ttcactcctt
     1741 ccatggtccg atcgatgata ccgtagagta gatgttattg caaatttact cgatctgaaa
     1801 gcgcatcgtt ggtcgttttt ctttcttgca atcgaaaggt cgcgcgttac aatgcgatga
     1861 ccggagcatg gaggatagga taatgaagaa cgcgtcagta ttccttcttc ttttctttgt
     1921 cgcgagcatc ttttcctcct gcgctggtag tcgtgttctg ggctcggacg gtgatgcggt
     1981 gcccgaccag gataacggtg gcccggaaga ggggagtgtc tgcgcggaag agaatgccat
     2041 gcgttgtcat gggaacgagg tgatgcaatg tgacggcgag atatgggtcg ttatcggtga
     2101 ttgtgccgat tacggcatgg aatgttatga gtacggcgat gaggcggtat gcaccaacaa
     2161 tgttcccgac ggtgacgccg atgaatggcc cgagctcgac ggggatattg atgaatatgt
     2221 gcccgcctgc ggcaacgcca tcatcgacac atgggaggaa tgcgacagca gtggggtcgc
     2281 ttgtacgact atcgacagcg tcacctatag cggcggcatg gccgactgtc tggaggactg
     2341 taccgggtac gacaccagtg actgccagat ggccatacct ccctgcagcc ttgacgcgac
     2401 ctttcttgac g
//
LOCUS       DAOQUE010000440         2402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_54101_length_2402_cov_2.854708, whole genome shotgun sequence.
ACCESSION   DAOQUE010000440 DAOQUE010000000
VERSION     DAOQUE010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54101_length_2402_cov_2.854708"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PKH10_11545"
     CDS             complement(<1..347)
                     /locus_tag="PKH10_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28796.1"
                     /translation="MDDAVTLHSTAFRRRRFLNWFPLGLAYALLYMGRYNLTVSKNAL
                     GDLMTKADFGDIFGIGAVVYAFSFLINGPLVDRIGGKRGMLVGVAGAAFANLLMGFYL
                     WYVLGLPDPASAPL"
     gene            complement(332..1651)
                     /locus_tag="PKH10_11550"
     CDS             complement(332..1651)
                     /locus_tag="PKH10_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28797.1"
                     /translation="MRPMPAVSFFLIFAFLPAFSISCSTNATHGTDDTPPLDEDALIG
                     SDDGLLPDTATDTITPDEYLPDTLLDEPDDIGADDEEIDEEVGEDAPFSDDLLPDDDG
                     TPPTLQGCLTGEYSLYHGNFHSHTGNSDGEESPPVAFAYARDVAGLDILAVTDHLEQL
                     YLYYVGIPDDEYPECRETASQLSSTTFLALCGYEYGSARMPDLIQSAGHSNVFFNDAL
                     LPMVQTDFHDYYASVAALPNIITQFNHPGDEDPTQTFGDFEFYPAIFQQMALYEFNGN
                     GPVWDLFFTALANGWWLSPVFNQDNHSANWGTANDQRTGIYMTALDMPSLYDAFAQRR
                     TFAANDKNATIRFMAEDACWMGSRLSGLSTYSLTVDVSDPDENDSFAAIELYDPLKNI
                     IATQDCTAATECLLSFELTVTGPTYVVARAVQTDGEYLVAAPIWMMP"
     gene            1825..>2402
                     /locus_tag="PKH10_11555"
     CDS             1825..>2402
                     /locus_tag="PKH10_11555"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28798.1"
                     /translation="MDNKQLTGMLVKRGLLSLPELKNLEEKALESGRGFRDLLSKEAN
                     IDEDKIVDILSEQYNVPKIRLADLDIEDDIISILNKQTCVRYKVMPISRIGNTLTVAM
                     SDPSNMNAQDDIKFLTDYNVEVVVAGEGEIIEAIEKYYGPIGTTSSAHADDDAALNLD
                     DLLSEMDEVQVEVYTAGLADGVRRFTDAMARPA"
BASE COUNT          514 a          666 c          766 g          456 t
ORIGIN      
        1 agtggtgccg atgccggatc gggtagaccg aggacatacc acagataaaa acccatgagc
       61 aggttcgcga acgccgcccc cgcaacgccg acgagcatgc cgcgtttgcc gccgatgcgg
      121 tccaccagcg gcccgttgat gaggaacgag aaggcgtaga ccaccgcgcc gatgccgaag
      181 atgtcgccga agtcggcttt ggtcatcagg tcacccagcg cgttcttaga aacggtcaga
      241 ttgtagcgcc ccatgtaaag cagggcgtag gcgagcccga gcgggaacca gttgaggaac
      301 cgccgccgcc ggaaggcggt gctgtgaagg gtcacggcat catccatatc ggcgcggcga
      361 cgaggtattc gccgtcggtc tggacggcgc gggccaccac ataggtcggg cccgtgacgg
      421 tcagttcgaa cgagaggagg cattcggtcg cggcggtgca atcctgcgtg gcgatgatgt
      481 tcttaagcgg gtcgtagagt tctatcgcgg cgaaggagtc gttctcgtcg ggatcgctca
      541 catcgacggt cagggagtat gtcgacagcc ccgacaggcg cgagcccatc cagcaggcat
      601 cctcggccat gaagcgtatc gtcgcgttct tatcgttggc cgcgaaggtg cgtcgctggg
      661 cgaaggcgtc gtagagcgac ggcatatcga gcgcggtcat atagatgccg gtccgctggt
      721 cgttcgcggt cccccagttc gccgaatggt tgtcctggtt aaacacgggc gagagccacc
      781 aaccgttggc gagcgccgtg aagaagaggt cccataccgg tccgttcccg ttgaattcgt
      841 agagggccat ctgttggaat atcgccggat agaactcgaa gtcgccaaag gtctgagtgg
      901 ggtcctcgtc acccgggtgg ttgaactgag tgatgatgtt cggaagcgcg gcgaccgagg
      961 cgtaatagtc gtggaaatcg gtctgcacca tcggcaggag tgcatcattg aaaaagacat
     1021 tgctgtgccc cgctgactgg atgaggtctg gcatccgggc gctcccgtac tcgtaaccac
     1081 acagggcaag aaaggtcgtt gatgacagtt gagatgccgt ctcacgacat tcggggtact
     1141 catcgtccgg tatgccgaca taatagaggt aaagctgttc gagatgatcg gtcaccgcga
     1201 ggatgtcgag tccggcgaca tcgcgggcat aggcgaaggc gacgggggga ctctcctcgc
     1261 cgtccgaatt gccggtgtgg gagtgaaagt tcccgtggta gagtgagtac tcgcccgtca
     1321 gacagccctg cagggtcggt ggagttccat catcatcggg cagaaggtcg tcagagaacg
     1381 gggcatcttc gccgacctct tcgtcgatct cttcatcatc ggcccctatg tcgtcgggct
     1441 cgtcgaggag agtgtcgggc agatattcgt cgggagtgat ggtgtcggtc gcagtgtcgg
     1501 gaaggaggcc gtcatcggac ccgatgagcg cgtcctcgtc gagagggggc gtgtcgtccg
     1561 tcccgtgcgt ggcattggtc gaacaggaga ttgaaaaggc tggcaggaag gcgaaaatga
     1621 gaaaaaatga aacagcgggc atcggtctca tggcggctac ctctcgcttg ttctcagcgc
     1681 ccccatctta ctttttaggg taagataagt caagcggagg caggggtgtg atgccgcaac
     1741 tccgatgtat cactgatttc ttgactcggc aagtatcggc ggctatactt ccccccgaat
     1801 ttgtcattga ccggggaccg ttcgatggat aacaagcaac tgaccggcat gctcgtcaag
     1861 cggggactcc tgtcgctccc cgaactcaag aatcttgagg agaaggcgct cgaatcgggc
     1921 cgcggcttcc gcgatctcct ttccaaagag gccaacatag atgaagacaa gatagtcgac
     1981 atactcagcg aacagtacaa tgtccctaag atacgactgg ccgatctcga catagaggac
     2041 gacatcatct ccatcctcaa caaacagacc tgtgtccgct acaaagtgat gccgatatcg
     2101 cgcatcggca acacgctcac cgtcgcgatg agcgaccctt cgaacatgaa cgcgcaggat
     2161 gacatcaagt tcctcaccga ctacaatgtc gaggtggtcg tcgccggcga gggagagatc
     2221 atcgaggcga tagagaagta ttacggaccc atcggtacga cctcctccgc ccacgccgat
     2281 gacgatgccg ccctcaatct ggacgatctc ctgtccgaga tggacgaggt gcaggtcgag
     2341 gtgtacaccg cgggcctggc cgacggcgtg cgccgcttca ccgacgcgat ggcgcgcccc
     2401 gc
//
LOCUS       DAOQUE010000441         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_54851_length_2381_cov_3.655632, whole genome shotgun sequence.
ACCESSION   DAOQUE010000441 DAOQUE010000000
VERSION     DAOQUE010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54851_length_2381_cov_3.655632"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /gene="dapD"
                     /locus_tag="PKH10_11560"
     CDS             complement(<1..538)
                     /gene="dapD"
                     /locus_tag="PKH10_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HNT28799.1"
                     /translation="MDAYEIIRFISESEKKTPVRAYLKGALDAVDLSLFREVYRGVDF
                     ALVMDEWNKVDAFLKMYHEQVHAERIDVDRRNSAVPLLDLKDINARIEPGAIIRDRVA
                     IGDKCVIMMGAVINIGSEIGEGTMIDMNVVVGGRVIVGKHCHIGAGSVLAGVVEPPSA
                     EPVRIGDHVVMGANAVVLE"
     gene            complement(548..1984)
                     /locus_tag="PKH10_11565"
     CDS             complement(548..1984)
                     /locus_tag="PKH10_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNT28800.1"
                     /translation="MDEKTKRVLAVFEEINKVPRRSKQEEKIVQWLVDWAKKRGFAVK
                     TDKVSNVLITVPATKGCEKAPVLVVQGHMDMVCEKTPDSPHDFTKDPIKCIIEGDWMH
                     ADKTTLGADNGIAIALALALAEDPEAIHPKLELLFTVDEETGLTGANAIEPGFLTGKI
                     LLNVDSEDEGIFTVGCAGGRDTKSVLPLSYEKAGAGTAFVKVGVGGLLGGHSGVDIKE
                     HRGNANIILARFLDGLRKQMPVALVTIKGGSAHNAIPRDAEAVIAVPEKSFEQVGGTV
                     AAFEKVLKNEFPKDSGLKLTALRDAVAAEAMDKKTTDAVIDLILALPHGIAAMSAEME
                     GLVETSNNLAILAVADKELTITTSQRSSVMSRLDWLTQKVETIARLGGATPHTGGGYP
                     SWQPNIKSPLLARCLKVYKELFKVDAKVEAIHAGLECGIIGAKYDGMDMISFGPTVKH
                     PHSPDEKLFVPSVGRYYDFMKALFKSYC"
     gene            2118..>2381
                     /locus_tag="PKH10_11570"
     CDS             2118..>2381
                     /locus_tag="PKH10_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28801.1"
                     /translation="MSRPLCHIPLSFLLALLCFFGCNPEESGPEGDPICGLYGVYSEI
                     LDACDCQIGYGGDFCDQCAKGYAGYPDCVKESGGSDGPAPDIDI"
BASE COUNT          390 a          764 c          714 g          513 t
ORIGIN      
        1 cttcgagcac gacggcgttg gcccccatga cgacatggtc gccgatgcgg accggctcgg
       61 ccgacggcgg ctccaccacc ccggcgagca ccgatccggc gccgatgtgg cagtgtttcc
      121 cgacgatgac gcgaccgccg acaacgacat tcatgtctat catcgtcccc tcgcctatct
      181 cggagccgat gttgatgacc gcccccatca tgatgacgca cttgtcgcct atcgccaccc
      241 gatcacggat gatggcgccc ggctcgatac gggcgttgat gtccttgagg tcgaggagcg
      301 gcaccgccga gttgcgccgg tcgacatcga tgcgttcggc gtggacctgt tcgtggtaca
      361 ttttgaggaa cgcgtccacc ttgttccact cgtccatcac gagagcgaaa tcgaccccgc
      421 ggtacacctc tcggaacagc gagagatcga ccgcgtcgag cgcccccttc aggtaggcgc
      481 ggaccggcgt ctttttctcc gattcgctga tgaaccggat gatctcgtag gcatccatga
      541 ccgcctccta acagtaactc ttgaatagcg ccttcatgaa atcgtagtag cggccgaccg
      601 acggcacgaa cagtttctcg tcgggcgaat gcgggtgctt cacggtgggg ccgaacgata
      661 tcatgtccat cccgtcgtac ttcgcgccga tgatgccgca ttcgagcccg gcgtgtatcg
      721 cctcgacctt cgcgtcgacc ttgaacagtt ccttgtagac cttcaggcac cgcgcgagga
      781 gcggcgactt gatgttgggt tgccacgacg gatagccgcc gccggtgtgc ggggtcgcgc
      841 cgccgagccg cgcgatcgtt tccaccttct gcgtcagcca gtcgagccgc gacatgaccg
      901 aactgcgctg gctcgtggtg atggtgagtt ctttatcggc caccgcgagt atggcaaggt
      961 tgttcgaggt ctcgacgagc ccctccatct cggccgacat cgccgcgatg ccgtgcggca
     1021 gggcgaggat gagatcgatg acggcgtcgg tcgtcttttt gtccatcgct tcggccgcca
     1081 ccgcgtcgcg cagggcggtc agtttcaggc cgctatcctt cgggaattcg ttcttcagca
     1141 ccttctcgaa ggccgcgacc gttccgccga cctgttcgaa cgacttttcc ggcacggcga
     1201 tgaccgcctc ggcgtcgcgc ggtatggcgt tgtgggcgct gccgcccttg atcgtgacga
     1261 gcgcgacggg catctgctta cgcagaccgt cgaggaaccg ggcgaggatg atattggcgt
     1321 tgccgcggtg ctccttgatg tcgacgcccg aatggccgcc cagcaggccg ccgacgccga
     1381 ccttcacgaa cgccgtcccc gcgccggcct tctcatagga aagcggcagg accgacttgg
     1441 tgtcgcggcc gccggcgcac ccgacggtga atatcccttc gtcctccgaa tcgacattga
     1501 gcaggatctt gccggtcaga aaaccgggct ctatggcgtt ggcgccggta agtcccgtct
     1561 cctcatcgac ggtgaacagc agttcgagtt tcggatggat cgcctccggg tcctcggcga
     1621 gggccagcgc aagcgcgatg gcgatgccgt tgtcggcgcc gagcgtcgtt ttatcggcgt
     1681 gcatccagtc gccctcgatg atgcacttga tcggatcttt cgtgaaatcg tgcggcgaat
     1741 cgggggtctt ttcgcagacc atgtccatgt gaccctgcac cacgagcacc ggcgccttct
     1801 cgcacccttt cgtggcgggg accgtgatga ggacattgga gaccttgtcg gtcttcaccg
     1861 cgaatccgcg ctttttcgcc cagtcgacga gccactgcac gatcttctct tcctgtttcg
     1921 agcgacgcgg caccttgttg atctcctcga acaccgccag cacccgtttc gtcttctcgt
     1981 ccatcctgcc ctccatcggt tgttgttgtt cggcgtcact tttcgctact gtatatgtta
     2041 aaaaaaagag gtttgcaatt ttcatggcct tcgcctataa tgaggcgttc gtcttaccct
     2101 tgaggatgga gttctcgatg agcaggcccc tctgccatat cccgctgtcg ttcctgcttg
     2161 ccctcctgtg cttcttcggc tgcaatcccg aggagagcgg cccggaaggc gatcccatct
     2221 gcggtctgta cggcgtctat agcgagatac tggatgcctg cgattgccag atcgggtatg
     2281 gcggcgactt ctgcgatcag tgcgccaagg gatacgccgg ctaccccgac tgcgtgaagg
     2341 agagcggcgg gagcgacgga cctgcccccg acatcgatat c
//
LOCUS       DAOQUE010000442         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_54995_length_2377_cov_4.033592, whole genome shotgun sequence.
ACCESSION   DAOQUE010000442 DAOQUE010000000
VERSION     DAOQUE010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54995_length_2377_cov_4.033592"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2377
                     /locus_tag="PKH10_11575"
     CDS             <1..>2377
                     /locus_tag="PKH10_11575"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT28802.1"
                     /translation="VNDAAQSWVVSFSDGFVNAYNTASTVGYARCVRGGTLPNSTFTE
                     ATVEGKIIVTDATTGLQWTKQYGPWQDWQAALNYCHTLDYGGATDWRLPNIEELKTLI
                     DDAIAVPSSGFPGMSPSYFWSSSSPVSDASHAWYWDAQLGPVHHTGKSAEYYAICVRS
                     PIDECLKWSNPCDDTEDAGGACTDEPTGYTCACSEYFEFFNGSCRDIDECMFGSDFCD
                     DNGDTAAACVNTSPGNDCECSDGYESVPYGSGSQYRTCVVIVPPTGVPCTGQTLCYNA
                     TEEMTCPTEGNDFYGQDAQYALLGSCVPRDYTIDGMVPEEVVTDDNTGLVWQRTLPAT
                     YAGCTGGAPAGSKCTWQNAIDYCDGLTYGGYDDWRLPSRKELATLPDYGRYNPAIDTA
                     IFPGTQSGFYWSSSSYASNTDDAWFVSFSYSDVTRSSKAETFYARCVRGDALPVSAFT
                     ESNVSGKVIVTDTVTGLVWTKECSETVTSWKNALAYCENLNYGGYTDWRLPNIDELTT
                     LIDDTVYDPASTFPGMPVQYFWSSSSSAVNTGYAFFVDFSFGEVGQNDKSNSTYARCV
                     RSEINECTTLTNPCDDNGDTGATCDDEAVGYTCTCSFNYTFDGTTCVADPVEVLCTGQ
                     TLCYNATEEMTCPTEGNAFYGQDSQYALLGACAPRDYTIDGMVPEEVVVDNVTGLIWQ
                     RTLPATYSGCTGSSGAYCAWQEAVDYCSNLTYGTYDDWRLPTMKELATLVDYGKFSPA
                     IDETTFPATPADGLFWSSFERINSTSNAWYAYLYNGDVGSDAKSSAYLHYAR"
BASE COUNT          512 a          669 c          673 g          523 t
ORIGIN      
        1 gtgaacgatg ccgcccaatc gtgggtcgtt tctttcagcg acgggttcgt gaatgcgtac
       61 aataccgcca gcacggtggg ctatgcccgt tgtgtgcgcg gcggcacact gcccaacagc
      121 acttttaccg aggcgacggt agagggcaag atcatcgtta ccgatgcgac gacggggctt
      181 cagtggacca aacagtatgg cccttggcag gattggcagg cggcgctcaa ttactgtcat
      241 accttggatt acggtggggc gaccgactgg cgtctcccga atatcgaaga actgaaaacg
      301 ctcatagatg atgccatcgc ggttccctct tctggtttcc cgggaatgtc gccctcatac
      361 ttttggtcct cttcgtcccc tgtgagcgat gcctcccatg catggtactg ggatgcccaa
      421 ttgggtccgg tgcatcacac cggcaagagc gcagaatact acgccatctg cgtccgttcg
      481 ccgatagacg agtgcctgaa gtggagcaac ccctgcgatg acaccgagga cgctggcggc
      541 gcctgcaccg acgaaccgac cggctacacc tgcgcctgct ccgagtactt cgagttcttc
      601 aacggcagtt gccgcgacat cgacgaatgc atgttcggga gtgacttctg cgacgacaat
      661 ggcgacaccg cggccgcctg cgtcaataca tctccgggga acgattgcga gtgttcggac
      721 ggatacgagt cggttcccta tggatccggt tctcagtacc gcacctgcgt cgttattgtt
      781 cctccgaccg gtgttccctg caccggtcaa acgctttgct acaatgctac cgaggaaatg
      841 acctgtccga cggagggcaa tgatttctac gggcaggatg cgcaatatgc tctgctggga
      901 tcatgtgtgc cgcgcgacta caccatcgac ggtatggtgc ctgaagaggt ggtcacagac
      961 gacaatacgg ggctcgtctg gcagaggacc ctcccggcga cctacgcggg gtgcaccggc
     1021 ggcgcaccgg ccggatcgaa atgcacttgg caaaacgcca tagactactg cgacggcctg
     1081 acctacggcg gctatgatga ttggcgtctc ccgtcccgta aggaactggc gacactgcct
     1141 gactacggtc gatataaccc ggcgatagac accgctatct tccccggtac gcagtctggt
     1201 ttttactggt cctcctcgtc ctacgcgagc aataccgacg atgcatggtt cgtgagtttc
     1261 tcctacagcg atgtgaccag aagcagtaag gccgaaactt tctatgcccg ttgtgtgagg
     1321 ggcgatgcat tgcccgtcag cgcattcacc gaatccaacg taagcggcaa agtgatcgtg
     1381 accgataccg ttacgggtct tgtctggacg aaagagtgta gcgaaacggt gacatcgtgg
     1441 aagaacgccc ttgcatactg cgagaacctg aactatggtg ggtacaccga ctggcgactg
     1501 ccgaacattg atgagttgac aacattgata gatgacacgg tctacgatcc cgccagcact
     1561 tttccgggaa tgccggtaca atatttttgg tcctcctcgt cctccgcggt caataccggc
     1621 tatgcatttt tcgtggattt cagcttcggg gaagtgggcc aaaacgataa gtccaatagc
     1681 acctacgccc gttgcgtccg ttcggagata aacgagtgta caacgctgac caacccgtgc
     1741 gacgacaacg gcgacaccgg cgcgacctgt gacgatgagg cggtcggtta cacctgcacc
     1801 tgctcgttca actacacctt cgacggtact acctgcgtcg ctgatccggt cgaggtcctt
     1861 tgcaccggtc aaacgctttg ctacaatgct accgaggaaa tgacctgccc gaccgaaggc
     1921 aacgcattct acggacagga ttcgcagtat gctctgctgg gagcatgtgc gccgcgcgac
     1981 tacaccatcg acggtatggt gcctgaagag gtggtggtcg ataatgtgac ggggttaata
     2041 tggcagagaa cacttccggc gacctacagt ggatgtaccg gtagttcggg tgcctactgt
     2101 gcatggcagg aggcagtgga ttattgtagc aatctgacct acggcactta cgacgattgg
     2161 cgattgccga ccatgaaaga acttgctact ctcgttgatt atgggaaatt ctcccccgcg
     2221 attgatgaga caacattccc ggcgacacct gccgacggtc ttttctggtc ctcctttgag
     2281 cgtattaaca gcacaagtaa cgcgtggtac gcttatctct ataacgggga tgtgggatcc
     2341 gatgctaaat cgtcagcata tcttcactat gccaggt
//
LOCUS       DAOQUE010000443         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_54996_length_2377_cov_3.855297, whole genome shotgun sequence.
ACCESSION   DAOQUE010000443 DAOQUE010000000
VERSION     DAOQUE010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54996_length_2377_cov_3.855297"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PKH10_11580"
     CDS             complement(<1..536)
                     /locus_tag="PKH10_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28803.1"
                     /translation="MAKILPVMVFVFLAFISCTTTEESELLDNEATGDNDDATAGDDL
                     NSEPMPSEGDEPVTDDEQNDGLIPDQDNAACEWDDPNGDDDEDGIPNQVEGCGDADLD
                     GAPSHLDTDSDGDGLLDSQECPDQPCQNSDQDDTPDFLDKDSDNDGLADKEEATLGTD
                     PYDKDTDGDGSDDLAEIV"
     gene            complement(645..1310)
                     /locus_tag="PKH10_11585"
     CDS             complement(645..1310)
                     /locus_tag="PKH10_11585"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT28804.1"
                     /translation="MRGMFIVALMVAVGVLVAAEAPKAPATETKKEGTTEVNKAETTP
                     PAKKEDAEKPKDPAPAPALPEPKEYEVKDEVVTDTKSGLMWQRKHADKSVTHKEAGDY
                     CEALTLGGHDDWRLPTISELRTLIVGCQSGTDACKVSDQCLAAKCLSVACSCQKLKGP
                     GENGFYWQPGVWQGEGKYFWSSSTQGSKNGKRAWDVGFNYGNVYDFDRTQKADVRCVR
                     SAK"
     gene            complement(1384..2118)
                     /locus_tag="PKH10_11590"
     CDS             complement(1384..2118)
                     /locus_tag="PKH10_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28805.1"
                     /translation="MRALFALLLFVPLLLGAEDPAMPVSPAVPEKITPEIARDVIARL
                     TAKYSKMYERYAGCQSTRHLEIKWYKPEKMELVKTEKVVYTRYDDFYEDLEYTTLSYE
                     VDGKPADPDDYDPHESKPGIPVFDRNGEKEYLRTVVGIENVKGQSAYKIQVTPRDPRN
                     EHFKGYIWATVAGLELVQFRGTAGKFRIGLHELSVFFEAKDFGDYYCFTAGHTEARIA
                     FFIFQPERILHFKWQATDFKPMTKSK"
     gene            complement(2157..2252)
                     /gene="ffs"
                     /locus_tag="PKH10_11595"
     ncRNA           complement(2157..2252)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKH10_11595"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2264..2354)
                     /locus_tag="PKH10_11600"
     tRNA            complement(2264..2354)
                     /locus_tag="PKH10_11600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2317..2319),aa:Ser,seq:gga)
BASE COUNT          460 a          703 c          654 g          560 t
ORIGIN      
        1 taaacgatct cggcaagatc atccgagcca tcgccgtcgg tgtccttatc gtacggatcg
       61 gttccgagag ttgcttcctc tttgtcggcc agaccgtcgt tgtcggaatc cttgtcgaga
      121 aaatcggggg tatcgtcctg atcgctgttc tgacagggct gatcagggca ctcctgacta
      181 tcaagaagcc cgtcgccatc cgagtcggtg tcgagatggc tgggggcacc atcaaggtcg
      241 gcgtcgccgc agccttccac ctggttcggt atcccatcct catcgtcatc accgttcggg
      301 tcgtcccatt cgcaggcggc gttgtcctga tcggggatca atccatcgtt ctgttcgtca
      361 tcggtgaccg gctcatcccc ttctgatggc attggttcgc tattgagatc atcgcctgcg
      421 gtcgcatcgt cattatcgcc ggtcgcctca ttgtcgagca attcgctctc ttctgtcgtc
      481 gtacatgaga tgaaagccag aaaaacgaac accatcacgg gcagaatctt cgccatgaca
      541 cgcctcccag attgttgtgg ttcagtacac caatggggtg attgtatcct accagagaaa
      601 caagtcaagg gagaaaggac ggcttatttt ttcaggggag agcgctactt ggcactcctc
      661 acgcaacgaa catcggcttt ctgggtccga tcaaaatcat atacattccc gtagttgaac
      721 ccgacatccc acgcccgctt gccgttcttg cttccctgtg tcgaggagga ccagaagtat
      781 ttgccttcac cctgccagac acccggttgc cagtagaacc cgttctcgcc gggccccttg
      841 agtttctgac aggaacaggc gacgctcaga catttcgccg cgagacactg atcgctcact
      901 ttgcaggcgt cggtcccgct ctggcatccg acgatgagcg tcctgagctc cgatatggtg
      961 ggaagccgcc aatcatcatg cccgccgagg gtaagggctt cacagtagtc ccccgcctct
     1021 ttgtgggtca cgctcttgtc cgcgtgtttc ctctgccaca tgagaccgct tttggtatcg
     1081 gtgaccacct cgtcctttac ctcgtactcc ttcggctcgg ggagcgccgg ggccggagca
     1141 ggatctttcg gcttctcggc atcctctttc ttcgctgggg gggtcgtttc cgccttattg
     1201 acctcggtcg tcccttcttt cttcgtctcg gtcgccggcg ccttgggggc ctcggccgca
     1261 acgagcacac cgaccgcgac catcagggct acgatgaaca ttccacgcat gatatcctcc
     1321 ctctgcgctg tggtatcgtt ccgatccgtt gtacctcttt ttcacgaaaa ggtcaacgaa
     1381 gattcactta ctttttgtca tgggcttgaa atcggtggcc tgccacttga agtggaggat
     1441 gcgttccggc tggaagatga agaaggcgat gcgcgcctcg gtatgtccgg cggtgaagca
     1501 gtagtagtcg ccgaaatctt tcgcctcgaa gaacaccgac agctcatgca ggccaatgcg
     1561 gaacttcccc gcggtaccgc ggaattgcac aagttcaaga ccggcgacgg tggcccagat
     1621 ataccccttg aaatgctcgt tgcggggatc gcgcggggtg acctgtatct tgtaggccga
     1681 ctgtcccttg acgttctcaa tgccgacgac ggtgcgcagg tattcttttt cgccgttccg
     1741 atcgaacacc gggatgcccg gttttgattc gtgcggatcg taatcgtcgg gatcggcagg
     1801 cttgccgtcg acctcgtagg agagggtcgt atactcaagg tcctcgtaga agtcgtcgta
     1861 acgggtatag accacctttt cggtcttgac cagttccatc ttctcgggtt tgtaccactt
     1921 gatctcgagg tgtcgggtcg actgacagcc ggcgtaccgc tcatacatct ttgagtactt
     1981 cgcggtcagc cgcgcaatga catcgcgagc gatctccggc gttatctttt cgggaacggc
     2041 cggagacacc ggcatcgccg gatcctcggc accaaggagc agcgggacaa aaagcagcag
     2101 agcaaaaaga gcgcgcatac cgtcaacctc tggtcatccg tgtcgcatga tcaactgaag
     2161 gccggggaca ccgcaacaca tgcgcggtcc ggtaccgttg cttcctttcg gacctagcgg
     2221 ggttcgcggg gcgcattgta cggctcccgg ccacaagaaa aaatggcgga gagggagaga
     2281 ttcgacctct cggtggcatt gctatcacac acaatttcca atcgtgctcc ttcggccaac
     2341 tcggacacct ctccacaaaa aaggaacatg gaggaga
//
LOCUS       DAOQUE010000444         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_56066_length_2349_cov_5.055362, whole genome shotgun sequence.
ACCESSION   DAOQUE010000444 DAOQUE010000000
VERSION     DAOQUE010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56066_length_2349_cov_5.055362"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1664)
                     /locus_tag="PKH10_11605"
     CDS             complement(39..1664)
                     /locus_tag="PKH10_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT28806.1"
                     /translation="MTTKTVLIYEYDNKHAKSLKKAFEQFDLTVRTARSEDDMQEFLG
                     TGAIDLFVIRAENPSISGLLLCKKIREISEYRSTPILILSSEATEQTFEKHRALDFAA
                     DHYMSLPQDAESILAAAHMLMPFLDGAEEAIEEVPAAAPVDDGQAAALKEEVKKLRSE
                     LQRREAELKEASSQSATLKKLREENEKLAAEAGELKEAIAQKAAQAKELEKAVSQGAD
                     LAVEFDRAAEQIKELQAETKRLEVELSAEREKSQAMNEDKTEVSRSLEQLKAQLKEES
                     DRAMKGASVEAEEIKKKYDEAKDELEDLRIVNKEMKDKVKTAEKDMEKMKAEHEAFTA
                     EKDALIKKAKEAEEAVVEQARTLKEEFEQKEKSAQAESKRLLDELQASYEEKLKPISD
                     ELKIKSEKLELLQAEKESAEIDLATARYKATQAEKQCGETKTRLEAAEKELNELRETV
                     LQFSSLSDDRDAALKDKEAALAEAAALTARIKVLEEEKDTVEKKVAELAAEVAKIAGL
                     EKTIAAKDADIAAAKEEATRLAEVIESVRKAVS"
     gene            complement(1684..2151)
                     /locus_tag="PKH10_11610"
     CDS             complement(1684..2151)
                     /locus_tag="PKH10_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28807.1"
                     /translation="MRQRLEDFCRDNPGARKIARALSAAAAVRLRVLPDTDVAFRKAD
                     EVFVLTDAATCGAVEYDMEVMLPAAMLEDFLAADPQTADAIILFFAENYYQEKYRDHI
                     DLRILSGVFRLTMKGYLGLIPLGGPALMSILRSKGFGSIGSITAALKGIGKKG"
BASE COUNT          343 a          774 c          665 g          567 t
ORIGIN      
        1 cgcacaggaa ctcgagcgtc agcgctatct gcatatcgtt acgaaacggc cttccgcacc
       61 gactcgatga cctcggcaag gcgcgtcgcc tcttccttgg ccgcggcgat gtcggcatcc
      121 ttcgccgcta tcgtcttttc gagcccggcg atcttcgcca cctccgccgc caactcggcg
      181 accttcttct cgacggtgtc cttctcttcc tccagcacct tgatgcgggc cgtgagcgcg
      241 gccgcttcgg cgagcgcggc ctctttgtcc ttcagggcgg cgtcgcggtc gtcgctgagc
      301 gacgagaact gcaggaccgt ctcgcgcagt tcgttgagtt ccttttcggc cgcctcgaga
      361 cgcgtcttcg tttccccgca ctgtttctcg gcctgcgtcg ccttgtaacg ggcggtggcg
      421 agatctatct cggcgctctc cttctcagcc tgcagcagtt cgagtttctc gctcttgatc
      481 ttgagttcgt cgcttatcgg cttcagtttc tcctcgtaac tcgcctgcaa ctcgtcgaga
      541 agccgcttcg attcggcctg cgcactcttt tccttctgtt cgaactcctc cttgagcgtc
      601 cgcgcctgct cgacgaccgc ctcttcggcc tccttcgcct tcttgatgag cgcatccttc
      661 tccgcggtga aggcctcgtg ttcggccttc atcttctcca tatccttctc ggccgttttg
      721 accttgtcct tcatctcctt gttgacgatc ctgaggtcct cgagttcgtc cttggcctca
      781 tcatacttct ttttgatctc ctccgcttcg accgacgcac ccttcatggc gcggtccgat
      841 tcctccttca gttgcgcctt gagttgttcg agcgaccggg agacctcggt cttgtcctcg
      901 ttcatcgcct gcgacttttc gcgctcggcc gacagttcga cctcgagccg cttcgtttcg
      961 gcctggagct cctttatctg ctcagccgcc ctgtcgaact cgaccgcgag atcggcaccc
     1021 tgcgacaccg ccttctcgag ttccttcgcc tgagccgcct tctgggctat cgcctccttg
     1081 agttcgcccg cctcggcggc gagtttctcg ttctcttcgc ggagtttctt cagcgtggca
     1141 ctctgcgacg aggcctcctt gagttcggcc tcgcgccgct ggagttcact gcgcagtttc
     1201 ttcacctcct ccttgagcgc cgcggcctgt ccgtcatcga ccggcgccgc cgccgggacc
     1261 tcttcgatcg cctcttccgc gccgtcgagg aacggcatga gcatgtgggc cgccgcgagt
     1321 atcgattcgg cgtcctgcgg gagcgacatg taatgatcgg ccgcgaagtc gagcgcgcgg
     1381 tgcttctcga aggtctgctc ggtcgcctcg ctcgaaagga tgagtatcgg cgtggagcgg
     1441 tattccgata tctcccgtat cttcttgcac agaagcaggc cgctgatgct cgggttctcg
     1501 gcccggatga cgaacaggtc tatcgccccg gtcccgagga attcctgcat gtcgtcctcc
     1561 gaccgggccg tccgcacggt gaggtcgaac tgctcgaacg cctttttgag cgacttggcg
     1621 tgtttgttgt cgtactcgta gatcagcacg gtcttagtgg tcatggcctt ctctccgcgt
     1681 tcgttatcct ttcttcccta ttcccttgag cgccgcagtt atcgaaccga tcgagccgaa
     1741 gcctttcgag cggagtatgc tcatgagcgc cgggccgccg agcggtatga gcccgagata
     1801 gcccttcatc gtgagccgga acacccccga cagtatcctc aggtcgatat ggtcgcggta
     1861 cttctcctga taatagttct cggcgaaaaa gaggatgatc gcgtcggccg tctgtggatc
     1921 agcggcgagg aaatcctcaa gcatcgcggc gggaagcatc acctccatgt catactcgac
     1981 ggcgccgcag gtcgcggcgt cggtcagcac gaacacttcg tccgccttgc ggaacgcgac
     2041 atcggtatcg gggaggacgc ggagccgcac cgcggcggcg gcgctgagcg cgcgggcgat
     2101 cttccgggcg ccggggttat cccggcagaa atcctcaagg cgttggcgca tgaacgatag
     2161 taccatcacc aaaaaccgta aagttctatc ctactaccat acaataaaaa ggtttgcaag
     2221 gaaattagcg gcggcgggcg tagagccatt ccttgagacg gttgggggcg atatcgggat
     2281 acaggcgggt caggagcgcc agcgcgtcct tcaccgagag cgcatcgagg atgcggtcgc
     2341 gttccgggt
//
LOCUS       DAOQUE010000445         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_56078_length_2349_cov_3.499128, whole genome shotgun sequence.
ACCESSION   DAOQUE010000445 DAOQUE010000000
VERSION     DAOQUE010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56078_length_2349_cov_3.499128"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..2154
                     /gene="uvrB"
                     /locus_tag="PKH10_11615"
     CDS             184..2154
                     /gene="uvrB"
                     /locus_tag="PKH10_11615"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNT28808.1"
                     /translation="MQSRFEIKAPFAPAGDQPRAIAQLTKNFRGGSKRELLLGATGTG
                     KTFVMANLIKELGLPTLVLAHNKTLAGQLYGEFKQFFPANRVGYFISYYDYYQPEAYI
                     PTTDTYIEKDSSRNDEIDRMRHFATSQLLEGNDCVIVASVSCIYGIGSPEAYRELSVE
                     LLRGMDLPMERLCRQLADIQYERTQFDLSRGKFRVSGDIVEIHPAYEENHLVRIEYFG
                     DTVEEILIVDPLEKRRIRSLDAVKVYPKSHYVAEKETMDRAVVAIRAEMEAQEALFKK
                     RNKLIEAQRIRERTENDLAMMGSVGFCSGIENYSRYLTGRGPGEPPPTLVDYFGKEFL
                     LIVDESHVTLPQVRGMFNGDRSRKEVLVEYGFRLPSALDNRPLNFDEFRERWDRVLFT
                     SATPARYEIEQVRQVTELINRPTGLLDPVLDILPAREEVPLLFDEIVKETGAGGKVLV
                     TALTKKMAEDLTDFLSGRGIRARYLHSDIDAMERLKIVMDLRRGLFDVLVGVNLLREG
                     LDNPEVTLVGILDADKEGFLRGGDSLIQTIGRAARNLNGRAILFADQVTDGMKKAIDE
                     TRRRRRIQEDFNAAHGITPQSILNKAILDIESHVPGKGKKAQKKLARKELARRIAELE
                     SEMKRAAEGWDFEYAAILRDRIFEYKGLLGEE"
     gene            complement(2159..>2349)
                     /locus_tag="PKH10_11620"
     CDS             complement(2159..>2349)
                     /locus_tag="PKH10_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT28809.1"
                     /translation="KGLNTALHYAASRGFVKAASLLIRKGVSLNDKDDKGRTPMVRAQ
                     FTGNYDIVDMLKRAGAYE"
BASE COUNT          467 a          704 c          739 g          439 t
ORIGIN      
        1 gccccatgat acggtgtttc tgaggatcct tccccccggt gtcctgaccc atggcggtgc
       61 ctcccgatcg ttggttcacg ccgttcatta taagggaagc atccccgttt gcaatttttc
      121 atcctcagac ccttgccttt gccgtcggtg tcggatagat tcgcgcgtga caggaggaga
      181 agcgtgcaaa gccgtttcga gatcaaggcg ccgttcgcgc cggcgggcga tcagccccgg
      241 gctatagctc aattgacaaa gaacttccgc ggcggttcga agcgcgaact gctcctcggc
      301 gctaccggca ccggcaagac cttcgtgatg gcgaacctca tcaaggaact cggcctgccg
      361 accctcgtac tcgcccacaa caagacgctt gcgggtcaac tgtacggcga attcaaacag
      421 ttctttcccg ccaatcgcgt cggctacttc atcagttatt atgactatta tcagcccgag
      481 gcctatattc cgacgaccga cacctacata gaaaaggatt cgtcgcgcaa cgacgagatc
      541 gaccggatgc gccattttgc cacgagccaa ctgctcgagg ggaacgactg cgtcatcgtc
      601 gcgtcggtat cctgcatcta tggcatcggc agtccggagg cctaccgcga actgtcggtg
      661 gaacttctgc gtggcatgga cctgccgatg gagcggctct gccggcaact cgccgatatc
      721 cagtacgagc ggacgcagtt cgatctttca cgcggcaagt tccgcgtgag cggcgacatc
      781 gtcgagatac accccgccta cgaagagaac catctggtgc ggatagagta tttcggcgac
      841 accgtcgagg agatactcat cgtcgatccg ctcgaaaaac gacgcatccg gtcgctcgac
      901 gcggtcaagg tctacccgaa aagtcactat gtcgccgaaa aggagacgat ggaccgggcg
      961 gtcgttgcta tccgggccga gatggaggcg caggaggcct tgttcaaaaa acgcaacaaa
     1021 ctcatcgaag cgcagcgcat ccgtgagcgg accgagaacg atctggcgat gatggggtcg
     1081 gtcggcttct gttccggcat cgagaactat tcgcgctacc tcaccgggcg cggtcccggc
     1141 gagccgccgc cgacgctggt ggattacttc ggaaaggaat tcctcctcat cgtggacgag
     1201 agccatgtga cgcttccgca ggtgcgcggc atgttcaacg gcgaccggtc gcgtaaggag
     1261 gtgctcgtcg agtacgggtt ccgtctgccg tctgcgctcg acaaccggcc gctcaacttc
     1321 gacgagttcc gtgaacggtg ggaccgcgtc ctgttcacct cggcgacccc cgcccgctat
     1381 gagatagagc aggtgcggca agtgaccgaa ctcatcaatc gaccgaccgg tctgttggac
     1441 ccggtgctcg atatcctgcc ggcccgcgag gaggtgccgc tcctgttcga cgagatcgtg
     1501 aaggagaccg gcgccggcgg caaggtgctc gtcaccgcgc tcaccaagaa gatggccgag
     1561 gacctgaccg atttcctgag cggacggggc atccgggccc ggtatctcca cagcgacatc
     1621 gatgcgatgg agcggttgaa gatagtcatg gatctgcgcc gggggctgtt cgatgtgctg
     1681 gtcggggtga acctcctgcg cgaggggctc gataacccgg aggtgacgtt ggtggggata
     1741 ctcgacgccg acaaggaggg gttcctgcgg ggcggcgatt cgctcatcca gaccatcggt
     1801 cgggccgccc gcaatctgaa cggtcgcgcc attctcttcg ccgatcaggt gaccgacggg
     1861 atgaagaagg cgatcgacga gacgcgccgc cgccgccgca ttcaggagga tttcaacgcc
     1921 gcgcacggca tcaccccgca gagcatactc aacaaggcca tcctcgacat cgagtcgcat
     1981 gtgccgggga aggggaagaa ggcgcagaag aaactcgccc gcaaggaact cgcccgtcgc
     2041 atcgccgaac tcgagtcgga gatgaagcgg gccgccgagg gctgggactt cgaatatgcg
     2101 gcgatactgc gcgaccgcat ctttgaatac aaggggctgt tgggagagga gtagggcgct
     2161 attcgtaggc gccggcccgt ttgagcatgt ccacgatgtc gtagttgccg gtgaactggg
     2221 cgcggaccat cggagtgcgc cccttgtcgt ccttgtcgtt gaggctgacc cctttacgga
     2281 tgagaagcga cgccgccttc acgaacccgc ggctggcggc atagtggagg gcggtgttga
     2341 gtcccttgt
//
LOCUS       DAOQUE010000446         2348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_56107_length_2348_cov_3.730484, whole genome shotgun sequence.
ACCESSION   DAOQUE010000446 DAOQUE010000000
VERSION     DAOQUE010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56107_length_2348_cov_3.730484"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PKH10_11625"
     CDS             complement(<1..726)
                     /locus_tag="PKH10_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28810.1"
                     /translation="MRIPLRLAEIVAASSFSEGNARYLQRNVLVVSIEATGPDWAVEA
                     MAPGETPARKVSFVARADGTLGSHDCSVHGVELCPDIIAAILKYNEIAIADLSPVTQL
                     VEIADASALVPTEERPIPLRIDKEGRIAFGAVPGRDAGTIGHLLERLDGTGRMLLLAV
                     SPGSFSYGDRWVRKGDLPIDVYYRRDGGTVRFSLPESFLFDRESGVIFDRENAAVFSL
                     SDGQRSLLTQLLAFAAPFDDMEQM"
     gene            complement(731..1393)
                     /locus_tag="PKH10_11630"
     CDS             complement(731..1393)
                     /locus_tag="PKH10_11630"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNT28811.1"
                     /translation="MSYPRTILAFLIVLLGVLPVAELSAQSDDLFLNFEALKSFKADF
                     KQVFTAKLTKKKSDPEVGVIYYKAPSFMRFDYLKGQGLDRQTFISDEGVSFINYEKKT
                     VMTKKGQGDYGDYLVFLKGVAEIKKKFSVKKGDVALSRKAGIAVDEGQSLFKLTPLKK
                     IQNLRYLFLVLQGKEVRSVAVIDEIGNINQLFFTSVQYDPKLESSVFTYTAPIGFEKS
                     QL"
     gene            complement(1412..>2348)
                     /locus_tag="PKH10_11635"
     CDS             complement(1412..>2348)
                     /locus_tag="PKH10_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28812.1"
                     /translation="PDADQAIASTDENIHDPREADAIWLATATGRVLSFDLTASGWMY
                     DPPEEGESILPILSYAGATIPEDGDSSVDNMPQITRIAAIRGLPFTVSYDMAYEALYE
                     ASSSAGGVWDPEQGRFSDERASFDRIVTGDPTGYAVLLTGPRSGAGCLIPPQEAVTLT
                     VRELVDDHVLEVDAGKWEDDLASCYEGTIAYGIFPKDRYLVRMVGPYGTLVERSAREM
                     PADWDPMLSREVSYRDEFLDIVIKRRTDKVRTARGLSFSFTVSPSISFFGPRSGESIK
                     GMAALPNGHMLLFAPLTASIFEYSPVFEETIVTYR"
BASE COUNT          481 a          723 c          690 g          454 t
ORIGIN      
        1 catctgctcc atatcatcga agggggccgc aaaggcgagc agttgcgtca aaagcgatcg
       61 ctgaccgtcc gacagggaaa aaacggccgc gttctcgcgg tcgaatatca caccgctctc
      121 ccgatcgaac aggaacgatt cgggtaacga gaaccgcacc gttcccccat cccgacggta
      181 atagacatct atcgggagat ctccctttcg cacccagcgg tcgccgtagg aaaaactgcc
      241 gggggatacc gcgagcagca gcatgcgtcc cgtcccgtcg agccgttcca gaagatgtcc
      301 gatcgtcccg gcgtccctgc cgggaactgc cccgaaggcg atccggccct ccttgtcgat
      361 cctgagcggt atcggccgct cctccgtcgg cacgagagcg gaggcgtcgg ctatctcgac
      421 caactgcgtc accggcgaca ggtccgcgat ggctatctcg ttgtacttga gtatcgccgc
      481 tatgatatcg gggcacagtt ccaccccgtg cacggaacag tcgtgcgagc ccagcgttcc
      541 atcggcgcgg gccacgaaac tcaccttccg cgcaggggtc tcgcccggcg ccatcgcctc
      601 gaccgcccag tcagggccgg tcgcctcaat cgaaacgacc agcacgttcc gttgcaggta
      661 acgcgcatta ccctcgctga aggaggaggc ggcgacgatc tccgcgagac ggagcggtat
      721 acgcatggtg tcagagttgc gacttctcga aaccgatcgg ggcggtatag gtgaataccg
      781 aggactccag tttcgggtcg tactggaccg atgtgaagaa caactgattg atgttcccga
      841 tctcgtcgat gacggcgacc gaccgcacct cttttccctg aaggacgagg aagagatagc
      901 gcaggttctg tatcttcttg agcggcgtca gtttgaaaag cgactgcccc tcatcgaccg
      961 cgatccccgc cttgcgggag agagcgacat cgcccttttt cacagaaaac ttctttttta
     1021 tctcggcgac gcctttgagg aaaacgagat agtcgccgta atcgccctgc cccttcttgg
     1081 tcatcaccgt cttcttctcg taattgatga aggaaacacc ttcgtcggat atgaaggtct
     1141 gacgatcgag cccctgcccc ttgagatagt cgaaccgcat gaaggagggg gccttgtaat
     1201 agatgacgcc gacctccgga tcgctcttct ttttggtcag tttggcggtg aacacctgct
     1261 tgaaatcggc tttaaaactc ttaagcgcct cgaaattcag gaacaggtca tcgctctgcg
     1321 ccgacagttc ggcgacaggg aggacgccca agagaacgat aaggaaggcg aggatggttc
     1381 tcgggtacga catctttctc tccttgatgc gctatcgata ggtgacgatc gtttcctcga
     1441 ataccgggct gtactcgaat atgctcgcgg tcagcggcgc aaaaagcaac atgtgcccgt
     1501 tcgggagcgc cgccatcccc ttgatcgatt cgcccgaccg cgggccgaaa aaggagatgg
     1561 agggtgagac cgtgaaggaa aaggaaagcc cgcgcgcggt ccggaccttg tccgtccgac
     1621 gctttatgac gatatcgagg aactcgtcgc ggtaggacac ctcccgggaa agcataggat
     1681 cccaatcagc gggcatctcg cgcgccgacc gttcgaccaa ggtcccgtac gggccgacca
     1741 tgcgtaccag gtaccggtcc ttcgggaata tgccgtaggc gatggtcccc tcgtaacagg
     1801 aggcgagatc atcttcccat tttcccgcat ccacctcgag cacatggtca tccaccagtt
     1861 cccgcaccgt cagcgtcacc gcttcctgcg gagggataag gcatcccgcg ccgcttcggg
     1921 ggccggtcag gagaacggca tacccggtcg ggtcgccggt gacgatacgg tcgaaggagg
     1981 cccgctcatc cgaaaaacgc ccctgttccg ggtcccacac cccgcccgcc gatgaggatg
     2041 cttcgtacag cgcctcgtag gccatgtcat acgaaacggt gaacggcagg cctcgtatcg
     2101 ccgcgatgcg ggttatctgc ggcatgttat ccaccgagga atcgccatcc tccggtatcg
     2161 tggcgccggc gtaagagagg atgggaagga tactctcccc ctcttcgggc gggtcataca
     2221 tccagccgct ggccgtcagg tcgaaggaca atacgcggcc ggtagcggtg gccagccata
     2281 tcgcgtcggc ttcgcgggga tcgtggatat tttcgtccgt cgaggcgatc gcctgatccg
     2341 catcgggg
//
LOCUS       DAOQUE010000447         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_56188_length_2346_cov_4.559144, whole genome shotgun sequence.
ACCESSION   DAOQUE010000447 DAOQUE010000000
VERSION     DAOQUE010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56188_length_2346_cov_4.559144"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PKH10_11640"
     CDS             complement(<1..1246)
                     /locus_tag="PKH10_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28813.1"
                     /translation="MTRILYLLAAGVILFVLACSEVKSTPNDEEGDDGDGIVTVDTDQ
                     KALPDTVTDTKPDGDTIPIDGDAPVVDDNTPITDGDTPPVENDTPVDDGDGILNDEDP
                     DEIDVEWPDTDSDTVICTANEEVACPYSGDPLTENVGLCKAGKKKCSPDGTAWSTCSG
                     EVLPAENDPCNDLDDNDCNGTVNDGYADGATGCICTPKEETACYTGPTGTEGVGICHA
                     GISTCNDYGNAWSSCTDEQTPVTEICGNTLNDDCVGDADDGLDEDLDGWTVCENDCCD
                     RTTQCENPAVVNPGAVEVTGDTIDNDCNDQTDEVPTGCSTVAKFSGTTALDLVNAMDI
                     CTTALNGSWGIVGTPTLIRVNGAGSIDNLQIAVSQQFGTDESNIAIFPPPPSAGVPGG
                     TMAVLSSGRARDMNDPDATALKT"
     gene            complement(1278..>2346)
                     /locus_tag="PKH10_11645"
     CDS             complement(1278..>2346)
                     /locus_tag="PKH10_11645"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNT28814.1"
                     /translation="VVDDTVDNNCNNQLNEDPQPACSSAAKFSGTTATDLVNAMDICQ
                     VAANGSWGIVGTPTITRASGSGAIDNLQVAVMTQFGTHASNLAIANATMASLSSGRAR
                     DSNDPNAPDSDTYQYYTGNPPADFSAPYGGGANLPQSNPNCDNGSGANDATMLTVQVK
                     VPTNALSLSFNFRFFSHEYWEYQCTTYNDFFIAMLYTGATGIPADKNISFDSNGRYIS
                     VNSEQFFTVCTSKEDGSGCGGTDYICSDGPAALAGTGFDIWEEGGCDEDDHYRGGGTK
                     WLSTVAPVVPGETITLKFVIWDTSDQALDSTVIIDNFKWSAQGTSGPSTFECWDLNKN
                     GTCDIATEDQSGDNVCNERDC"
BASE COUNT          344 a          673 c          800 g          529 t
ORIGIN      
        1 aggtcttcaa tgcggtggca tcggggtcgt tcatgtcgcg ggcccggccc gaagagagga
       61 ccgccatggt gccgccgggt actccggctg atggcggggg cgggaagatg gcgatgttcg
      121 attcgtcggt gccgaattgc tgtgataccg ctatctggag attgtctatt gagccggccc
      181 cgttgacgcg gatcagcgtc ggggtcccga cgatgcccca cgagccgttc agcgccgtgg
      241 tgcagatgtc catcgcgttc acgaggtcaa gggccgttgt gccgctgaac ttcgccaccg
      301 tgctgcagcc ggtcggaacc tcgtcggtct ggtcgttaca gtcgttgtcg atggtatcgc
      361 ccgtgacctc gaccgcgccg ggattgacga ccgccgggtt ctcgcactgg gtggtcctat
      421 cgcaacagtc gttctcgcaa acggtccagc cgtcgagatc ttcgtcgagc ccgtcatcgg
      481 cgtcgccgac gcagtcgtca ttgagggtgt tgccgcatat ctcggtgacg ggggtctgtt
      541 cgtcggtaca ggaactccac gcgttcccgt agtcgttgca ggtgctgatc ccggcgtggc
      601 agatgccgac gccttcggtc cccgtggggc cggtgtagca ggccgtctct tccttcggcg
      661 tacagataca gccggtggcg ccgtccgcgt agccgtcgtt gaccgtgccg ttacagtcgt
      721 tgtcgtcgag gtcgttgcag gggtcgttct ccgcgggaag cacctcgccg gaacaggtgc
      781 tccatgcggt gccgtcgggc gagcatttct ttttgcccgc cttgcagagg ccgacattct
      841 cggtcagcgg gtcgccggaa taggggcagg cgacctcctc gttggcggtg catatcacgg
      901 tgtcggaatc ggtatcgggc cactcgacat cgatctcgtc gggatcctcg tcgttgagta
      961 tgccgtcgcc gtcgtcgacc ggggtgtcgt tctcgacggg cggggtgtcg ccgtcggtga
     1021 taggggtgtt atcgtcgacg accggagcgt cgccgtcgat cgggatcgtg tcgccgtcgg
     1081 gtttcgtgtc ggtcacggtg tcgggaaggg ccttttggtc ggtatcgacc gttacgatgc
     1141 cgtcgccgtc atcgccctcc tcgtcgttcg gcgtgctttt cacctcggag caggcgagta
     1201 cgaaaaggat cactcctgcc gcaagcaggt acaggatgcg ggtcatgcat cacccccttt
     1261 ctgttgagat atctttctta gcagtcgcgt tcgttgcaga cattatcgcc cgactggtcc
     1321 tcggtggcga tatcgcaggt gccgttctta ttgagatccc agcactcgaa ggtcgaagga
     1381 ccgctcgtgc cctgcgcgct ccacttaaag ttgtcgatga tgacggtcga gtcgagtgcc
     1441 tgatcggagg tgtcccatat cacgaacttg agcgttatcg tttcgcccgg gacgaccggc
     1501 gcgacggtcg aaagccattt cgtgccgccg cctctgtaat ggtcgtcctc atcgcagccg
     1561 ccctcttccc atatgtcgaa tccggtgccg gcaagcgcgg cggggccgtc tgaacagatg
     1621 tagtcggtgc cgccgcaccc gctcccgtcc tccttgctcg tgcagaccgt gaagaactgc
     1681 tcggagttga cgctgatgta tctgccgttc gaatcgaacg atatattctt gtcggccggg
     1741 atgcccgtcg cgccggtgta gagcatcgcg atgaagaaat cgttgtatgt ggtgcactgg
     1801 tattcccagt attcatgcga gaagaaccgg aagttgaagg acaaggaaag ggcgttggtc
     1861 ggcaccttga cctgcacggt cagcatcgtc gcgtcgttgg cgcctgagcc gttgtcgcag
     1921 ttgggattgc tctggggcag gttggccccg ccgccgtagg gggcgctgaa atcggcgggc
     1981 ggattgccgg tgtaatattg gtaagtgtcg gaatcggggg cgttggggtc gttcgagtcg
     2041 cgagcgcggc ccgaggagag cgaggccatc gtggcgttcg cgatggcgag gtttgacgcg
     2101 tgggtaccga actgggtcat caccgcgacc tgcaggttgt cgatggcccc cgagccgctg
     2161 gcgcgggtta tggtcggtgt gccgacgatg ccccacgagc cgttcgcggc gacctgacag
     2221 atgtccatgg cgttcacgag gtcggtcgcg gtggtgccgc tgaacttcgc ggcgctcgag
     2281 catgcgggtt gcgggtcttc gttgagttgg ttgttgcagt tgttgtccac ggtgtcgtcc
     2341 acgacc
//
LOCUS       DAOQUE010000448         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_56491_length_2338_cov_3.822164, whole genome shotgun sequence.
ACCESSION   DAOQUE010000448 DAOQUE010000000
VERSION     DAOQUE010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56491_length_2338_cov_3.822164"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PKH10_11650"
     CDS             complement(<1..738)
                     /locus_tag="PKH10_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNT28815.1"
                     /translation="MTDPSLKEKTVTGATWVAVEKWGQQAVQFVITVLIARILSPADY
                     GIIGILLVFLMLSNVLIDSGFGAALVRKQEATDADYATVFYLNLIVSVACYGLLYLAA
                     PFIADFFNEPQLTAVARVLGLTVILNSFALIQMTILQKRMDFRPQAVVALVSALGSGA
                     VGVAMAYGGFGLWALVGQSLASASLQAMLYWLFGSWRPALVFSRASLVSLFAFSSNLL
                     VSSLINVLFKSVVPVIIGKYYSTRDLGF"
     gene            complement(769..>2338)
                     /locus_tag="PKH10_11655"
     CDS             complement(769..>2338)
                     /locus_tag="PKH10_11655"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT28816.1"
                     /translation="EHDVPTLLNEGKLERIWAPLSELLYSFRHLLLREAAYDMQLRSR
                     LKTIHRVVAETMERIYPDDDKHASEMAFHFERAEERDKTRRYLAKAADHARAHYQNED
                     AIRFYTKLLDYVETPAEELDIRHKLGGVYKLIGKWKEAEDSYRAALDKATALQDNVRV
                     AANMQDLSYLMLEKGRYEESRELLDKALDLYRSLGDRTGECMVLGYIGLIHYYKGDLD
                     KAVEFFNRRLTIAKELGDRANIMLSYRYLGGVGYYRADYRTAQEYYRQVLTFAEELGA
                     ELDMAIAKTNLGLSCSYLNDFENALRYYRESLEVYQKFGSKFYIAYTQNNMGELLFWM
                     GKYEDATTSFKEQRDIAAELGSSRHIAMANNMLGNVSKRQRRHDDAEKYYDRAIKIAT
                     DLSLKNLLGEFCFEKGELLFDMGRIDEAEKLTREAVEISEAVGRKDFLFKARLFKERI
                     RARTDTAAAIATLEELLGDCQTADQKAIVYYYLCEFTGDRGHCEKALALYEELFKEAP
                     RADYRDRIDTLRKN"
BASE COUNT          443 a          645 c          763 g          487 t
ORIGIN      
        1 gaagccgagg tcgcgggtcg agtagtactt gccgatgatg accgggacga cgcttttgaa
       61 caggacattg ataagcgagg agacgaggag gtttgaagag aaagcgaaca gggatacgag
      121 cgaggcgcgg gaaaagacga gcgccggtcg ccacgagccg aacagccaat agagcatcgc
      181 ctgcaacgag gcgctggcga gcgactgccc gacgagcgcc cagagcccga acccgccgta
      241 ggccatggcg acgccgaccg cacccgaccc gagagccgag acgagcgcga cgacggcctg
      301 cggacggaaa tccatccgtt tctgcaggat ggtcatctga atgagagcga aggagttgag
      361 tatgacggtc agaccgagca cccgggcgac ggcggtcagt tgcggttcgt tgaagaagtc
      421 ggcgatgaag ggcgcggcca gatagagcag gccgtaacag gcgaccgata cgatcaggtt
      481 aaggtagaat acggtcgcat aatcggcgtc ggtcgcctcc tgtttccgga cgagggcggc
      541 gccgaacccg ctgtcgatga ggacattcga cagcatgagg aacacgagca gtatgccgat
      601 gataccgtaa tcggcggggc tgagtatccg ggcgatgagg acggtgatga cgaattggac
      661 cgcctgttgg ccccatttct cgacggcgac ccatgtggcg cccgtgaccg tcttttcctt
      721 cagggatgga tcagtcatag gaacatcccc ccgaacaccg cagtgggatc agttcttccg
      781 cagggtgtcg atgcggtcgc ggtagtcggc gcgcggcgct tccttgaaca gctcctcgta
      841 cagcgcgagc gccttctcgc agtgtccccg gtcgccggtg aattcgcaga gatagtagta
      901 cacgatcgcc ttttgatcgg cggtctggca gtcaccgagc agttcttcga gcgtcgcgat
      961 ggccgccgcg gtgtctgtgc gggcccggat ccgctctttg aaaaggcgcg ccttgaagag
     1021 gaaatctttg cgtccgaccg cttcgcttat ctcgaccgcc tcacgggtaa gtttttccgc
     1081 ctcgtcgatc cgccccatgt cgaagaggag ttcgcccttc tcgaagcaga actcgccgag
     1141 cagattcttg agcgacagat cggtcgctat cttgatggcg cggtcgtagt atttctccgc
     1201 gtcgtcgtgg cgccgctggc gcttgctcac attaccgagc atattgttcg ccatcgcgat
     1261 gtggcgcgaa ctgccgagtt cggccgcaat atcgcgctgt tctttgaatg aggttgtcgc
     1321 gtcctcatat ttgcccatcc agaagagcag ttcccccatg ttgttctggg tgtaggcgat
     1381 gtagaatttc gaaccgaatt tctggtacac ctcaagactt tcgcggtaat agcgcagggc
     1441 gttctcgaag tcgttgaggt acgagcagga gaggccgaga ttggtcttgg cgatggccat
     1501 gtcgagttcg gcgcccagtt cctcggcgaa ggtgagcacc tgtcggtaat attcctgcgc
     1561 ggtgcggtaa tcggcacggt aatagccgac cccgccgagg taacgatagg aaagcatgat
     1621 gttggcgcgg tcgcccagtt ccttggctat cgtgagccgc cggttgaaga actcgaccgc
     1681 cttgtcgagg tcgcctttgt agtagtggat gagcccgatg tagccgagca ccatgcattc
     1741 accggtccgg tcgccgagcg atcggtagag gtcgagcgcc ttgtcgagca gttcgcgcga
     1801 ttcttcgtac cggccctttt cgagcatgag ataactgaga tcctgcatgt tggccgcgac
     1861 gcggacattg tcctgcaggg cggtcgcctt gtcgagcgcg gcacggtacg aatcctcggc
     1921 ttccttccat ttgccgatga gtttgtagac gccgcccagt ttgtggcgga tgtccagttc
     1981 ctcggccggg gtctcgacat agtcgagcag tttggtatag aatcgtatcg catcctcgtt
     2041 ctggtagtgg gcgcgggcat gatcggccgc cttggcgagg tagcggcggg tcttgtcgcg
     2101 ttcctcggcc cgctcgaaat ggaacgccat ctccgaggca tgcttgtcgt catccgggta
     2161 gatacgctcc atcgtctcgg cgacgacgcg atggatggtc ttgagccggc tccgtaactg
     2221 catatcgtag gctgcctcgc gcaggagcag gtggcggaag gagtagagca gttcggagag
     2281 cggcgcccag atgcgttcga gtttgccttc gttgagtagc gtcgggacat cgtgttcg
//
LOCUS       DAOQUE010000449         2333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_56672_length_2333_cov_3.294996, whole genome shotgun sequence.
ACCESSION   DAOQUE010000449 DAOQUE010000000
VERSION     DAOQUE010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56672_length_2333_cov_3.294996"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKH10_11660"
     CDS             complement(<1..222)
                     /locus_tag="PKH10_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28817.1"
                     /translation="MTSFIRWLSSRKAIIGFAILLFVTFLSSEWFLLSEAQRTADSAV
                     NHLETTLSAKGDPRAVEQARDFAETYRTDG"
     gene            complement(307..1836)
                     /locus_tag="PKH10_11665"
     CDS             complement(307..1836)
                     /locus_tag="PKH10_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT28818.1"
                     /translation="MRMLLILAFPLMTATLTAADDPTVHYYGFTGEPVELRRVDDGST
                     PWPVVSRGATPMPLTGRIGLKRDGADRIEFVELAPGSDPFAEARRLAEKPGVRWAQPE
                     WLVKVRPLAAPHDPYYPREWHLDQLGAAAAWEITAGSPHTAVAVVDSGVDRAHPDLDA
                     NLDAGKDLTGSGSTDCDRPASGGDASLIAAHGTCVAGLIAAEKNNGVGIAGICPLCRV
                     IPVKLLRYDETMIPVSRIYDAIVAAVDAGADIVNNSWGDEDTDAAGNCIAVPLDSYRA
                     EAIRYAREHGRAGRGALTVWAAGNSSCDAALNENLKLPDILVVSALAADDAFTGYSNY
                     GDPVDLCAGAANWTTDITGRDGINDRLAAGPTGAYDHTNQFSGTSAAAAVLSGVGGLL
                     YSANPLLFFAEALTCLREHTIAPTADCPAGGWTSSSGDPYAPEGAQRSPCFGFGLPDI
                     EALLSGARDGSCGLVYDGCDTDAACGAGFTCDTVRRECRKTLQPERPAASGGCALVIM
                     G"
     gene            complement(1836..>2333)
                     /locus_tag="PKH10_11670"
     CDS             complement(1836..>2333)
                     /locus_tag="PKH10_11670"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HNT28819.1"
                     /translation="FAVSLFVAVNRDLARFVLPYLFSKKTIFTVFMTAGIVYFERIVR
                     EAGVATAIYEALRHTNAEIVVFTLSLVIGLLTGISIAFISIVFPIISPFLIHDGSLDM
                     PLLAFAYTGGFTGVMLSPTHLCLIVTTDYFKTRLSAVYPLIMRAGLLMALGGGIYYVI
                     LRMVT"
BASE COUNT          415 a          685 c          833 g          400 t
ORIGIN      
        1 cccgtcggtg cgataggtct cggcgaagtc ccgcgcctgt tcgaccgcgc gcgggtcgcc
       61 cttggccgaa agcgtcgttt ccaagtgatt gaccgcgctg tcggcagtgc gttgtgcctc
      121 ggagaggagg aaccattcgg aggagaggaa ggtgacgaaa agcagtatcg cgaaaccgat
      181 gatggctttc cggctactca gccagcgaat gaaagaggtc atgaccgcac tctctatcgt
      241 tgataaccgg gcgattgtaa catgatgcgg gagataatca actttttgat ggggccggcc
      301 gtcgtgtcat cccattatga cgagcgcgca gcctcccgag gcggcggggc gttccggctg
      361 tagggtcttc ctgcattcgc ggcggaccgt gtcgcaggtg aaaccggcgc cgcacgccgc
      421 gtcggtgtcg catccgtcgt agacgaggcc gcaggatccg tcgcgggcgc cggaaagcag
      481 ggcctcgatg tcgggaagcc cgaagccgaa acagggcgat cgttgcgccc cctccggggc
      541 gtacgggtcg ccggaggatg aggtccagcc gcctgcggga cagtcggccg tcggggcaat
      601 ggtatgttct cgtagacagg tgagcgcctc ggcgaaaaac aggagcgggt tggcactgta
      661 caggagcccg ccgacgcccg acaggaccgc ggccgccgcg gaggtaccgc tgaactggtt
      721 ggtgtgatcg tatgcgccgg tcggtccggc ggcgaggcgg tcattgatcc cgtcgcgccc
      781 cgtgatatcg gtcgtccagt tcgcggcacc ggcgcagagg tcgaccgggt cgccgtagtt
      841 cgagtatccg gtgaaggcat cgtcggcggc gagcgcggag acgacgagga tatcgggtag
      901 tttcagattc tcgttgagcg ccgcgtcgca cgacgaatta ccggcggccc agacggtgag
      961 ggcgccgcga ccggcgcgcc cgtgttctct ggcgtagcgt atcgcctcgg cgcggtaact
     1021 gtcgagcggt accgcgatgc agttccccgc cgcgtcggtg tcctcgtcgc cccaactgtt
     1081 gttcacgata tcggcgccgg cgtcgaccgc ggcgacgatg gcgtcgtaga tgcggctgac
     1141 cggtatcatc gtttcgtcgt agcgaagcag tttcaccggt atcacgcggc ataacggaca
     1201 gatcccggcg atgccgacgc cgttgttctt ttcggcggcg ataagtcccg ccacgcaggt
     1261 accgtgcgcc gcgatgagcg atgcgtcgcc gcccgaggcc ggtcgatcgc aatcggtcga
     1321 gccgctcccc gtgaggtcct ttcccgcatc gaggttcgcg tcgagatcgg gatgggcgcg
     1381 gtcgacgccc gaatcgacga ccgcgacggc ggtgtgcggc gaccctgccg ttatctccca
     1441 cgccgccgcc gcgccgagtt ggtcgagatg ccactcgcgc ggatagtagg ggtcgtgcgg
     1501 tgcggcgagc ggccgtactt tcaccagcca ctcgggctgg gcccaccgga cgcccggctt
     1561 ttcggcgagg cgtcgcgcct cggcgaacgg gtcgctgccc ggcgcgagtt cgacgaactc
     1621 tatccggtcg gcgccgtcgc gtttgagccc gatgcggccg gtgagaggca tcggcgtcgc
     1681 gccgcggctc acgaccggcc acggtgtcga gccgtcgtcg acgcgccgaa gttcgaccgg
     1741 ttctcccgtg aagccgtaat agtggaccgt cgggtcatcg gcggccgtaa gggtcgccgt
     1801 catgagcggg aacgcaagga taaggagcat ccgcatcagg tcaccatcct taatatcaca
     1861 tagtagatac cgccgccgag tgccatgagg agtccggcgc gcatgatgag cgggtagacc
     1921 gccgagagtc gcgtcttgaa gtaatcggtc gtcacgatga ggcagagatg ggtgggtgag
     1981 agcatgacgc cggtgaagcc gccggtgtag gcgaaggcga ggagaggcat atcgagcgag
     2041 ccgtcgtgga tgaggaaggg gctgatgatg gggaagacta tcgaaatgaa ggcgatcgag
     2101 atgccggtga gcagtccaat aacgagcgag agcgtgaaca ccacgatctc ggcgttggtg
     2161 tgccggagtg cctcgtagat ggcggtcgca acgcctgcct cgcgcacgat gcgttcgaag
     2221 tagacgatgc cggcggtcat gaacacggtg aagatcgtct ttttgctgaa cagataggga
     2281 agcacgaacc gggcgagatc gcggttgacg gcaacaaaca atgagaccgc gaa
//
LOCUS       DAOQUE010000450         2330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_56799_length_2330_cov_3.804396, whole genome shotgun sequence.
ACCESSION   DAOQUE010000450 DAOQUE010000000
VERSION     DAOQUE010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56799_length_2330_cov_3.804396"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKH10_11675"
     CDS             complement(<1..300)
                     /locus_tag="PKH10_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HNT28820.1"
                     /translation="MNGQQATVVALDPKSGKLTLDVQGTIRTMRADEIRYLDHAYATT
                     VHSSQGLTCERVFVNMDTKKELVLGQEAYYVGISRAKETARIYTDAPEKLPDALKK"
     gene            complement(297..1433)
                     /locus_tag="PKH10_11680"
     CDS             complement(297..1433)
                     /locus_tag="PKH10_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT28821.1"
                     /translation="MQRGRGAVAPVTDADRVTEAVVEFAGRKGFSLSEGQQKSTQLIL
                     TTRDRVIGIEGYAGTGKTTMLELVRQEAERNGYAIRGYCPSAAAAKVLQKETGIVSTT
                     LASHLLAQKKEGTPPTGGKELWIIDEASMVSTLQAADLLKAAEKQQARIVLLGDRKQL
                     ASVEAGKPFAQLIDNGMAYEKITEIRRQKNVRLREAVYEAMDPEKIVSSLRKLDIEKV
                     PDQHKRLDRMVGDYLALPAADRAKTLLLTSTNDDRRALNGAVRQGLKKEGTLTGPALE
                     TGILITTGLTKAELGKIESYHEGQMLRFNRKYKTLGSRTATTPISPGWTAERDSSRWR
                     RPTAVNSPSTSPATGTWNCSTPRNGNSGKGTPSASRRTTNNRTL"
     gene            complement(1737..2312)
                     /gene="mobF"
                     /locus_tag="PKH10_11685"
                     /pseudo
     CDS             complement(1737..2312)
                     /gene="mobF"
                     /locus_tag="PKH10_11685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
BASE COUNT          316 a          683 c          757 g          574 t
ORIGIN      
        1 cttcttcagg gcgtcgggca acttctcggg ggcgtcggta tagatgcgcg ccgtctcctt
       61 cgcgcggctg atcccgacat agtacgcctc ctgcccgagg accagttcct ttttcgtatc
      121 catgttgaca aatacccgct cgcaggtgag tccctgcgaa ctgtggacgg tcgtggcgta
      181 ggcgtggtcg aggtagcgga tctcgtccgc gcgcatagtg cggatggtcc cctgtacatc
      241 gagcgtgagt ttcccgcttt ttggatcgag cgcgaccacg gtggcctgct gaccgttcac
      301 aaggtcctgt tgtttgtcgt tcttcgtgaa gcggatggtg tcccctttcc ggagttcccg
      361 tttctcggtg tcgaacagtt ccaggttcct gtggcgggtg atgtcgaggg tgagttgacg
      421 gccgtcggtc gtctccaccg cgaggagtcc ctttcggcgg tccaccccgg cgatatgggc
      481 gtggtcgccg ttctcgaccc gagcgtctta tatttccggt tgaaccggag catctgccct
      541 tcatggtaac tctctatctt gccgagttcc gctttggtga ggccggtcgt gatgaggatg
      601 ccggtctcga gggcgggccc ggtgagcgtg ccctccttct tgaggccctg acggaccgct
      661 ccgttgaggg cgcgccggtc gtcgttggtc gaggtgagga gcagggtctt cgcgcggtcg
      721 gccgccggga gggcgaggta gtcccctacc atgcggtcga gccgtttgtg ttggtcggga
      781 accttctcga tatcgagttt cctcagcgac gagacgatct tctcggggtc catcgcctcg
      841 tagaccgctt cgcgtagtcg gacattcttc tggcgccgta tctcggtgat cttctcgtag
      901 gccatcccgt tgtcgatcag ttgcgcgaac ggtttgcccg cttccaccga ggcgagttgc
      961 ttgcggtcgc cgagcaggac gatacgcgcc tgttgtttct ccgccgcctt gaggaggtcg
     1021 gccgcctgca gggtcgagac catcgaggct tcgtcgatga tccagagctc cttgccgccg
     1081 gtcggagggg taccctcctt cttttgggcg aggagatggg aggcgagggt cgtcgaaacg
     1141 ataccggtct ccttctggag taccttcgcg gccgccgcgg aggggcaata cccccggata
     1201 gcgtagccgt tgcgttccgc ttcctgcctg acgagttcca gcatggtggt tttcccggtg
     1261 ccggcgtagc cctctatccc gatgacgcgg tcccgcgtcg tgagaatgag ttgggtggac
     1321 ttctgctgtc cttcggaaag ggagaagcct ttccttcccg cgaattcgac gacggcctcc
     1381 gtgacgcggt cggcatcggt taccggagcg accgctcccc taccccgctg catgagggcg
     1441 atattcacct tctcggtatc gagatgcatg tgggtcacca cggtgcggct cttggtggtg
     1501 atgatctcct gttttttccg ggcatgggcg taggcctctt caacctcctg aatggtgcag
     1561 cggcccatcc cgtcggcgag cgccgcggtg aggaggtccc gctccttcat gatggtgttc
     1621 cgttcggaga gtttcgcgac cgcgtaccgg agtgagccgg cggcgttcga ccggtcgccg
     1681 tacaccgggt cgcggggaac ggtccgttcc ggggtcttgg gaccgatacc gagcgtcgcg
     1741 ctttcatggc gccacgccgc atagagttcg gcgcggtcta tctcgacctt ttcgcggcgc
     1801 gtcttgagcg ttgcctcctc cgccttgcga gaggaggaaa ggccgtactg cgccatgtat
     1861 tccttgatct gttgacgccg ggtgctgaaa tggtccaact gttccctgtc gaaaccggtc
     1921 agttccatca tgccgttcgg ttgcggttct atacggtagc ctttcgcggt caggtgacgg
     1981 gcaagggaac tctggtagat gaccccgacc agtttctggt gtttatagaa ttcctcgttg
     2041 ttgagcgctt tccattgccc gttacggtcg agcgtcgcgt tcactactac gcagtgggtg
     2101 tgcagttgcg ggtcgagttc acggctggtg tcgtgctcga atttcacggc gaggaggttc
     2161 cccgacccct cgacgatctt ttcgccggtc ttcttgttca tggtgcgggt ggtggcgaga
     2221 tgttcctcga tgtaccggag cgcatggtcg accgccgcgc ggtgcgcctc tatcatgtcg
     2281 gcgttcagtc gtttatcgtc tatggcgagc gctgagaccg atacgcttta
//
LOCUS       DAOQUE010000451         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_57301_length_2317_cov_4.171972, whole genome shotgun sequence.
ACCESSION   DAOQUE010000451 DAOQUE010000000
VERSION     DAOQUE010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57301_length_2317_cov_4.171972"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PKH10_11690"
     CDS             <1..854
                     /locus_tag="PKH10_11690"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HNT28822.1"
                     /translation="VTSEFRYRDPIVTPDDLVIVISQSGETADTLAALRAAKERKVPV
                     LAVCNVVGSTIAREADTTLFTYAGPEIGVASTKAFTTQLVMLYLIALKVGIAKGQLAK
                     SAVHVHIRGLAELPEIIESVLKESGRIEKIAHDLYRSSDFLFLGRGMNYPIALEGALK
                     LKEISYIHAEGYPAGELKHGPLALVDRNLPVVAVMPANPMAGKMFSNLKEVAARDGRI
                     VLLTTEGSDEAGDIAEAVIPMPPMSEWQEPIAYIVPLQLLSYHVAVNKGTDVDQPRNL
                     AKSVTVE"
     gene            854..1327
                     /locus_tag="PKH10_11695"
     CDS             854..1327
                     /locus_tag="PKH10_11695"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNT28823.1"
                     /translation="MFNLVLISPEIPPNTGNVGRSILATGGKLHLVKPLGFKLDDYHL
                     RRAGLDYWEDIDLTVWEGLDDIVAQVPAEKLHFFSTRGKHRYDRHQYRSGDWFLFGNE
                     SRGIDPAVLALHADRTFHIPMSNLAVRSLNLSSSAAVVLFEALRQNGFGAPGGAA"
     gene            1324..>2317
                     /locus_tag="PKH10_11700"
     CDS             1324..>2317
                     /locus_tag="PKH10_11700"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNT28824.1"
                     /translation="MKLIYRLFNIREEEQTRAGLLFLYNFLVVATYVQGRIVRDTLFL
                     KRYDVSQLPLMYIFVAASVSMLTWLYTRRSSLYRLDTLISTILFLSISLTVVFIGLIH
                     VGFQFSYPGLYILVEVIGAFMTFQFWSFTNELLDSREAKRILSFIGIGGIIASLVAGY
                     SVKWLVHLVKVENLLFINALFMVLCLYLVRRMGETYKSKLQRSVVIRSVQKTARHAPV
                     SLFTQPYFRYIALVTVVTFIIVNFVDYQFKMEAREHFSEKELAGFFGMVYAFASGFFS
                     LFFQLIATSWLLRWSIFLSLLILPAMITGFSAMFFLIPGIWFITMARASDFAFRY"
BASE COUNT          433 a          721 c          673 g          490 t
ORIGIN      
        1 gggtgacgag cgagttccgg taccgcgacc cgatcgtcac ccccgatgat ctggtcatcg
       61 tgataagcca gagcggcgag acggccgaca cgctggcggc cctgcgcgcc gcgaaagagc
      121 gcaaggtgcc ggtgctggcg gtctgcaatg tggtcgggtc gaccatcgcg cgcgaggccg
      181 acacgacgct ttttacctat gccgggcccg agataggtgt cgcatcgacc aaggcattca
      241 cgacgcaact cgtgatgctc tacctcatcg cgctcaaggt cggcatcgcc aaggggcaac
      301 ttgccaaaag cgcggtccat gtccatatcc gcgggctcgc cgaattgccc gagatcatcg
      361 agtcggtgct gaaggagagc ggccgcatcg aaaagatagc gcacgacctc taccggtcga
      421 gcgacttcct gttcctcggg cgtggcatga attacccgat agcgctcgaa ggggctctga
      481 aactcaagga gatatcctac atccacgccg aggggtaccc ggcaggcgaa ctcaagcacg
      541 ggccgctcgc gcttgtcgac cgtaacctgc cggtcgtcgc ggtgatgccc gccaacccga
      601 tggcggggaa gatgttcagc aacctcaagg aggtcgcggc gcgcgacggt cgcatcgtgc
      661 tcctgacgac cgaaggttcc gacgaggcgg gagacatcgc cgaggcggtc atcccgatgc
      721 cgccgatgtc ggagtggcag gaaccgatcg cctacatcgt gcctctgcaa ctgctcagtt
      781 accatgtcgc ggtcaacaag gggaccgatg tcgatcagcc ccgcaacctc gccaagagtg
      841 tgacggtgga atgatgttca atctggtact catcagcccc gagataccgc cgaacaccgg
      901 caacgtgggg cgaagcatac tcgccaccgg gggaaaactg cacctcgtga agccgctggg
      961 cttcaaactt gacgactatc acctccgtcg tgccggcctt gattactggg aggatatcga
     1021 cctgacggtg tgggagggtc ttgacgatat cgtggcgcag gtcccggccg aaaaactcca
     1081 tttcttctcg acgcggggca aacaccggta tgaccggcat cagtatcggt cgggcgactg
     1141 gttccttttc ggcaacgagt ctcgcgggat agatcctgcg gtgctcgcgc tccacgccga
     1201 ccgcaccttc cacatcccga tgagcaatct tgcggtgcgg agtctcaacc tgtcgagttc
     1261 cgctgcggtg gtgctgttcg aggcgcttcg ccagaacggc ttcggtgcgc cgggaggggc
     1321 ggcatgaagc tcatctaccg gttgttcaac atccgcgagg aggagcagac ccgcgccggg
     1381 ctcctgttcc tgtacaattt cctcgtcgtc gctacctatg tgcagggccg catcgtccgc
     1441 gatacgctgt tcctgaaacg gtacgatgtc tcgcaactgc cgctcatgta catctttgtc
     1501 gccgcaagcg tttcgatgct cacctggctc tacacgcggc gcagttcgct ataccggctc
     1561 gataccctca tcagcaccat cctgttcctg agcatctcgc tcaccgtcgt gttcatcggg
     1621 ctcatccatg tcggattcca gttcagttat ccgggacttt acatcctcgt cgaggtcatc
     1681 ggcgctttca tgaccttcca attctggtcg ttcaccaacg aactgctcga ctcacgcgag
     1741 gcgaaacgga tactcagttt cataggcatc ggcggcatca tcgcgagcct cgtggccggc
     1801 tattcggtca agtggctcgt ccatctggtc aaggtcgaga acttgctgtt catcaacgcg
     1861 ctcttcatgg tgttgtgcct ctacctcgtg cgccggatgg gggagaccta caagtcaaaa
     1921 ctccagcgga gcgtcgtgat acggtcggtc cagaagaccg ccagacacgc gccggtaagt
     1981 cttttcaccc agccctattt ccgctatatc gctctggtga ccgtcgtcac ctttatcatc
     2041 gtgaacttcg tggactacca gttcaagatg gaggcgaggg agcatttctc ggaaaaggaa
     2101 ctggcggggt tcttcggcat ggtctacgcc ttcgcgagcg gcttcttctc gctgttcttc
     2161 caactcatcg ccacctcgtg gctcctgcgc tggagcatat tcctgagtct gctcatcctg
     2221 cccgcgatga tcaccgggtt ctcggcgatg ttcttcctga taccgggtat ctggttcatc
     2281 acgatggcgc gcgcctctga tttcgccttc cgctaca
//
LOCUS       DAOQUE010000452         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_57542_length_2311_cov_2.745124, whole genome shotgun sequence.
ACCESSION   DAOQUE010000452 DAOQUE010000000
VERSION     DAOQUE010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57542_length_2311_cov_2.745124"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PKH10_11705"
     CDS             complement(<1..718)
                     /locus_tag="PKH10_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28825.1"
                     /translation="MQVVERVIEEIRTMFPDWERTVPPKIWPLIEQTAREVPLPELEG
                     FLERYLSAGKEWGFHPYAPFARRILKNVLYVITEQVLSGAGNLEKALDLVRDGSASRL
                     ILLTNHLSYADANLLATLFHPMLEAKGFGDDFSVVVGPKVYNERFKAFASLQFNSLLI
                     AQSLTVASGDAALPLKEIARAAKKVMARNESSNMSSTRIGATRTLRCSGSSGSPALHH
                     AANARTCRASTSMAVPGSFTA"
     gene            complement(800..1447)
                     /gene="lexA"
                     /locus_tag="PKH10_11710"
     CDS             complement(800..1447)
                     /gene="lexA"
                     /locus_tag="PKH10_11710"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNT28826.1"
                     /translation="METKRSPLTASQQKILAAIGELTERRGFSPTTREIASALGISAA
                     SVHEQIARLEKKGHLRRSPRQARSLEVVGDVSRGESADIPDIHLARVPIVGRIAAGSP
                     LLAEENCEGEILVDAGSIGTGRFFALKVVGDSMVNAGILDGNLVIVRRQPVAESGDII
                     AALLGDEATVKRLKIAADGVFLMPENERHRPIPVTDREDFSILGKVVSTVSLPVK"
     gene            1607..>2311
                     /locus_tag="PKH10_11715"
     CDS             1607..>2311
                     /locus_tag="PKH10_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28827.1"
                     /translation="MKSLNVALFAGWSAVLLLSGCSGDVYKIDRDAVRADKDSAVTGG
                     DTDEALADDLQNDQEGEDADGPGNDAPLKETDSPGDPDEDGYYPDDAYPDDEESDGYL
                     PDDMNTDDGEPDGAYPDEADPDETTPDGDLPDDAQPDDATPDSDNETPDEDLPQICTP
                     LEIVDCPYSGDPDKIDVVPCKVGTKQCNLNGTEWSDCSGVVLPAATDPCTDSVITTVT
                     ARSTTATMTAPPVAPVC"
BASE COUNT          475 a          670 c          743 g          423 t
ORIGIN      
        1 cagccgtgaa cgatccgggt acggccatcg aggtgctggc ccggcaagtg cgggcgttcg
       61 ctgcatggtg caatgcgggc gagcccgacg agccgctgca ccgcaacgtt cgcgtggcac
      121 cgatacgcgt ggacgacatg ttcgacgatt cattcctggc catgaccttt ttcgcggcgc
      181 gggcgatctc cttgagcggc agggccgcat caccgctcgc cacggtgagc gactgggcga
      241 tgagcaggga gttgaactgg agcgaggcga aggctttgaa tctctcgttg tagaccttgg
      301 ggccgacgac gaccgagaag tcatcgccga agcctttcgc ctcgagcatg ggatggaata
      361 gggtcgcgag cagattggcg tcggcgtaac tcagatggtt ggtcagcagg atgagccgcg
      421 atgccgatcc gtcacgcacc agatcgagcg ccttttcaag gttgccggcg ccgctgagta
      481 cctgttcggt gatgacataa aggacatttt tgaggatgcg ccgcgcgaag ggggcgtagg
      541 gatggaagcc ccattccttg ccggctgaaa gatatcgttc gaggaagccc tcaagttccg
      601 ggagcggcac ctcgcgggcg gtctgttcga tgagcggcca tatcttgggc ggcacggtcc
      661 gttcccaatc ggggaacatg gtccgtatct cctcgatcac ccgttcaacg acctgcatga
      721 ataactctcc gttcctaaag gcaaaaggtc accggagcac tcttataatg gaagaaaggg
      781 gcaaaatcaa gttatgcggt cacttgacgg gaagcgagac ggtcgagacg accttgccga
      841 gtatgctgaa atcctcgcgg tcggtcaccg gtatcggtcg gtgccgttcg ttctcaggca
      901 tcaggaacac gccgtcggct gctatcttga gccgttttac agtggcctcg tcgccgagaa
      961 gcgccgcgat gatgtcgccc gattcggcca ccggctggcg gcgtacgatg acgaggttgc
     1021 catcaagtat cccggcgttc accatcgagt cgccgaccac cttgagcgcg aagaaccggc
     1081 cggtaccgat ggacccggcg tcgacgagga tctctccctc gcagttctct tcggcgagca
     1141 gcggggaccc ggcggcgatg cgtccgacga tggggacgcg ggccagatgg atgtccggta
     1201 tgtccgccga ctcgcctcgt gacacatcac cgacgacctc aagcgatcgc gcctgtcgcg
     1261 gagagcggcg caggtgaccc ttcttctcca gccgcgcgat ctgttcatga acgctggccg
     1321 ccgatatgcc cagcgcggag gctatctcgc gggtggtagg ggagaacccg cgccgttcgg
     1381 tcagttcgcc gatggcggcg aggatcttct gctgtgaagc ggtgagcggc gatcgtttcg
     1441 tctccatggc gcctcctgcc tgacaagtgg tcaacggacg gataatatgc ctgacaaatg
     1501 aaaggtcaag taaaatcttt tcagcgcgga cggctcactc aaaaacttgc taataccaag
     1561 gtaatacata aaatcgacgg ggtctcggat ggtggaggtg ctcgttatga agagccttaa
     1621 tgtcgcgttg ttcgcagggt ggtcggcggt cttgttgctc agcggttgct ccggagatgt
     1681 ctataagatc gaccgggacg ccgttcgtgc cgacaaggat agcgccgtga caggtggcga
     1741 taccgatgag gcgctggctg atgatctgca gaacgatcag gagggtgaag acgccgacgg
     1801 gcccgggaac gatgccccgc tcaaggagac cgattctccg ggcgatcccg acgaggacgg
     1861 atattatccc gacgacgctt atcccgatga cgaggagagc gacggatacc tgcctgacga
     1921 catgaacacg gatgacgggg aacccgatgg agcgtatccc gacgaggccg accccgatga
     1981 aacgacaccc gacggcgatc tgccggacga tgctcagccc gatgatgcca cgcccgacag
     2041 cgacaacgag acacccgacg aagacctccc tcagatatgc accccgctgg agatagtcga
     2101 ctgtccctac tccggcgacc cggacaagat agatgtcgtg ccctgcaagg tcgggacgaa
     2161 acagtgcaac cttaacggca ccgagtggag cgactgttcc ggcgtggtgc ttccggcggc
     2221 gaccgacccc tgcaccgaca gcgtgataac gactgtaacg gcacggtcaa cgacggctac
     2281 gatgacggcg ccaccggttg cacctgtatg c
//
LOCUS       DAOQUE010000453         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_57750_length_2305_cov_4.866667, whole genome shotgun sequence.
ACCESSION   DAOQUE010000453 DAOQUE010000000
VERSION     DAOQUE010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57750_length_2305_cov_4.866667"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PKH10_11720"
     CDS             complement(<1..1047)
                     /locus_tag="PKH10_11720"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNT28828.1"
                     /translation="MVFRIEVGLRGDLKDPHGRMIQRRLKSDLGINSVTEIRVFKVYL
                     VDIEKADEPKVRRFMNEVLTDPITEVAHLDEDLTEAFDFDWLVEVGFLPGVTDNEGRT
                     ARWAFNALFPGLTGEGDAVYAGRKYLVRGTANAKDIERCATGLLCNKLIQRYKLIGRS
                     GWDKGERMGAAIPAVTGTDRPEVRLLPLEIADEALEKLSKERVLALSLEEMKAVQAYY
                     RRPRTQNKRKELGFPELPTDCEIEILAQTWSEHCKHKIFNAEIFYTDATSNTAFKVDS
                     LFKSYIKKTTEDLRPALPWLRSVFDDNAGVIEIDEGTLLAVKVETHNSPSALDPYGGA
                     LTGIVGVNRDIIGCG"
     gene            complement(1140..>2305)
                     /locus_tag="PKH10_11725"
     CDS             complement(1140..>2305)
                     /locus_tag="PKH10_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014693542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28829.1"
                     /translation="DENTPSAVGTAIDWQNLSVGQNHTCGIRTDGSLYCWGYNRSGQL
                     GNGTIRVDEKKTLVPSTARSDWKEMTVAHLHTCGITLDGALYCWGGNDHGQLGDGTTT
                     IRDNPVRVGNTSDWNMIAARYHHTCGIKGTGDLYCWGLNADGQLGDGTTIDKYSPTRI
                     GMDNDWQLVSTGSTSTCGIRAGGKMYCWNKFSLTPKVIGIDSDWLSIDVGYSHACAVK
                     TDGSLYCMGANHLGQLGDGTIQDKTEFVHIISGSDWQTISVGGEHTCGIRAGGELYCW
                     GSNLSYQLGNGMIQNEITPIRISGANDWQVVAAGSFMTCGVRAEGKLYCWGDGYPTPT
                     RRNAESAWSVVDVEETHRCAIKTSGSLYCAGYNNVGQLGIGNDTLAPEKVLNP"
BASE COUNT          455 a          662 c          632 g          556 t
ORIGIN      
        1 gccgcacccg atgatgtcgc ggttgacgcc gacgatgccg gtcagcgccc ctccgtacgg
       61 gtcgagcgcc gaggggctgt tgtgggtctc caccttgacg gcgagcaggg tgccttcatc
      121 tatctcgatg acccccgcgt tatcgtcgaa gaccgacctg agccacggga gggcgggccg
      181 gaggtcctcg gtggtcttct tgatgtaact cttgaaaagc gaatcgacct tgaacgcggt
      241 gttcgatgtc gcgtcggtgt agaatatctc ggcattgaat atcttgtgct tgcagtgctc
      301 cgaccaggtc tgggcgagta tctcgatctc gcagtcggtc ggcagttcgg ggaacccgag
      361 ttccttccgt ttgttctggg tgcgcgggcg gcggtagtag gcctgcaccg ccttcatctc
      421 ctcaagcgag agtgcgagca cccgctcctt ggaaagtttt tcgagcgctt cgtcggcgat
      481 ctcgagtggc aggagtcgca cctcgggccg gtcggtcccg gtgacggccg gtatggccgc
      541 gcccatccgt tcgcccttat cccagccgct ccgtccgatg agtttgtagc gctggatgag
      601 tttattgcac agcaggccgg tggcgcaccg ttcgatatcc ttcgcgttcg ccgtgccgcg
      661 cacgaggtat tttcgtccgg catagacggc gtccccttcg cccgtcaggc ccgggaagag
      721 cgcgttgaat gcccagcggg cggtccgtcc ctcgttgtcg gtcacgccgg gaaggaaccc
      781 gacctcgacg agccagtcga aatcgaatgc ctcggtgagg tcctcgtcga gatgggccac
      841 ctcggtgatg gggtcggtga gcacctcgtt catgaagcgg cgtaccttgg gctcgtcggc
      901 cttctcgatg tcgacgagat agaccttgaa cacgcggatc tcggtgaccg agttgatgcc
      961 aagatcgctc ttgagtcgcc gctgtatcat cctgccgtgg gggtctttga gatcgccgcg
     1021 caggcccact tcgatacgga ataccatcgc acactcctca ttgcggaaag aacggacgag
     1081 gggcattcta tgtaatcggt ccgattattc aagaaaaagg gcggatgctg ggataggggt
     1141 caggggttca agactttttc tggcgccaga gtgtcgttgc cgatacctag ttgaccgaca
     1201 ttgttatagc cggcacaata gagactcccg cttgttttaa tggcacatct gtgggtctcc
     1261 tcaacatcaa caacactcca agcactttca gcattgcggc gcgttggcgt gggataaccg
     1321 tcaccccaac aatagagttt gccttccgcc cgtacgccac aggtcatgaa agatccagca
     1381 gcaacaacct gccagtcatt ggcgccactg atacggatcg gtgttatctc gttctggatc
     1441 attccattac ccaattgata ggagaggtta gatccccaac agtaaagctc accacctgct
     1501 cgtatcccac aggtatgttc ccccccgaca gatatcgtct gccaatcact tccggagatt
     1561 atgtgaacaa attcggtttt gtcttggatt gttccatcac ccagctgccc cagatgattc
     1621 gctcccatac aatagaggct accatcggtc tttaccgcac acgcgtggct gtatccaaca
     1681 tcgatcgaga gccaatcact gtcgatacca ataaccttgg gggtgagaga aaatttattc
     1741 cagcagtaca tcttgcctcc ggctcgtatc ccgcaggtgc tggtcgaacc ggtggaaacg
     1801 agctgccaat cattgtccat gccgatacga gtcggactat atttatcgat ggttgtgccg
     1861 tctcccagtt gaccgtcagc gtttagaccc cagcaataaa gatctccggt acccttgatg
     1921 ccacaagtat ggtgatatct tgcggcgatc atgttccagt cgcttgtgtt cccgacacgg
     1981 acgggattat ctcggatcgt tgttgtgccg tctcctaatt ggccatgatc atttccgccc
     2041 caacagtaaa gagctccgtc aagggtgatc ccgcaagtgt gaagatgcgc gacggtcatc
     2101 tctttccagt cgcttcgcgc agtactaggg accaatgtct ttttttcatc cactcttatt
     2161 gtgccgtttc ccagctgccc gctacgatta tatccccaac aataaagtga accatcggtt
     2221 cgtatcccgc aggtgtggtt ttgtccaaca gataagtttt gccaatcaat cgctgtgccg
     2281 acagcggaag gtgtattctc atcca
//
LOCUS       DAOQUE010000454         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_58769_length_2279_cov_3.692896, whole genome shotgun sequence.
ACCESSION   DAOQUE010000454 DAOQUE010000000
VERSION     DAOQUE010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58769_length_2279_cov_3.692896"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKH10_11730"
     CDS             <1..810
                     /locus_tag="PKH10_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNT28830.1"
                     /translation="DPDLLAGLDNFCWPDPVYHEEKNPDGKEKLAALVRANMALRDLC
                     LPYRIPLISGKDSMKNDYGSGKDKISVPPTLLFTLVSRMEDVERMVTMDLKNPGDLIY
                     LVGATADEMGASEYFAYHGIRACGYAPKVRPAEFMDAYRKLHAAMKEGLVASAHDLSD
                     GGLAVAIAESAFAGGLGVKIDLTKVPRKGFIERDDFVLFSESNGRLLVSVSPAYREQF
                     EQVMAGSECALIGEANDSGFMKVSGTAGRALISLPCEELWRRWKSPLEKLK"
     gene            822..1619
                     /gene="purQ"
                     /locus_tag="PKH10_11735"
     CDS             822..1619
                     /gene="purQ"
                     /locus_tag="PKH10_11735"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HNT28831.1"
                     /translation="MGKAQVLILSGYGVNCEVETGWAFEKAGADRVTIAHINRAIAGE
                     VSLDDFQVLVFPGGFSFGDHIASGRVLGVKVKQELGPRLTEFVRSGRLVLGICNGFQV
                     LVKTGLLPGDDEQVFAPQKVTLAHNDSARYEDRWVLLKANPKSRSPFLRDVGTISCAV
                     RHGEGKFLALDNEMARMKAHNMIAFQYVDKKGIPTQEYPKNPNGSEQAIAGITNKRGN
                     ILGLMPHPEVFISKTQHPHWTRLPDLEEHGAGLKIFRNAVEYVKNEL"
     gene            1616..1762
                     /locus_tag="PKH10_11740"
     CDS             1616..1762
                     /locus_tag="PKH10_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28832.1"
                     /translation="MKKKLAILLVVSLVTTIFLWIFELTDDLHRFNMFVIELVVEKAT
                     ALFN"
     gene            1841..>2279
                     /locus_tag="PKH10_11745"
     CDS             1841..>2279
                     /locus_tag="PKH10_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNT28833.1"
                     /translation="MMNGVTAVLLAGGRGARMGAQVPKQELELDGRKIYRIAAERFAS
                     FTIVDAIMFVCPAERLDAYKAGLAGIPKVRWVIAGGAERHDSVWNALEALRNDPPAVV
                     LMHDAVRPFVSGRTVAEVARVAAEHGAATAAARAVDTLYEVRDG"
BASE COUNT          456 a          713 c          702 g          408 t
ORIGIN      
        1 gatcccgacc tgctcgcggg gctcgacaac ttctgctggc ccgacccggt ataccatgaa
       61 gagaagaacc ccgacggcaa ggaaaaactc gcggcgctcg tccgcgccaa catggcgctc
      121 cgcgacctct gtctccccta ccgcatcccg ctcatctccg gcaaggacag catgaagaac
      181 gactacggga gcggcaagga caagatatcg gtcccgccga ccctgctgtt cacgctcgtg
      241 tcccgcatgg aggatgtcga acggatggtg acgatggatc tcaagaaccc cggcgacctc
      301 atctacctcg tcggtgcgac cgccgacgag atgggcgcgt ccgaatattt cgcctaccac
      361 ggcatccgcg cctgcggcta cgctccgaag gtgcgtccgg ccgaattcat ggacgcctac
      421 cgcaaattac acgccgccat gaaagagggt ctcgtcgcct cggcgcacga cctttcggac
      481 ggcggactcg cggtggcgat cgccgaatcg gccttcgccg ggggtctcgg cgtcaagata
      541 gacctgacca aggtgccgcg caagggtttc atcgaacgcg acgacttcgt gctgttctcc
      601 gaatcgaacg gccgtctgct cgtgtcagtg tcccccgcct accgtgaaca gttcgaacag
      661 gtcatggcgg ggagcgaatg cgccctcatc ggcgaggcga acgactcggg gttcatgaag
      721 gtgagcggga ccgccggccg ggcgctcatc agccttccgt gcgaggagtt atggcgccgc
      781 tggaagtcgc ctcttgagaa actgaagtaa cgaggatacc catgggcaag gcacaggtac
      841 tcattctcag cggctacggc gtcaactgcg aggtcgagac cggctgggcg ttcgagaagg
      901 cgggcgccga ccgcgtgacg atagcgcata tcaaccgggc catagcgggc gaggtgtcgc
      961 tcgacgattt tcaggtgctc gtattccccg gcggcttctc gttcggtgat catatcgcat
     1021 cgggccgggt gctcggggtc aaggtgaaac aggaactggg gccgcgcctc accgaattcg
     1081 tccggtcagg gcggttggtg ctcggcatct gcaacgggtt tcaggtactc gtcaaaacgg
     1141 ggcttctccc cggcgacgac gaacaggtgt tcgccccgca gaaggtgacg ctcgcccaca
     1201 acgactcggc ccgctacgag gaccgctggg tgctcctgaa ggccaatccg aaaagccgct
     1261 cgcccttcct gcgcgatgtc ggcaccatct cctgcgcggt ccggcacggc gagggcaaat
     1321 tcctcgcgct cgacaacgag atggcgcgga tgaaggcaca caacatgatc gccttccagt
     1381 atgtggataa aaagggcatc ccgacgcagg aatacccgaa gaacccgaac gggtccgaac
     1441 aggcgatagc gggcatcacc aacaaacgcg gcaacatcct tggcctcatg ccgcaccccg
     1501 aggtgttcat atcgaagacc cagcaccccc actggacccg cctgcccgat ctcgaggaac
     1561 acggggcggg gctcaagata ttccgcaatg ccgtcgaata cgtaaagaac gaactgtgaa
     1621 gaagaagctc gccatactac tcgtcgtctc gctcgtcacc accattttcc tgtggatatt
     1681 cgaacttacc gacgacctgc accggttcaa catgttcgtc atcgaactcg tcgtcgagaa
     1741 ggcaacggct ctctttaact agccgaaaat ttgctttggg tcccgttacc gctataatgg
     1801 gcccgttcct taatttaatg gtaatcgttt tgatggatcg atgatgaatg gtgttacggc
     1861 ggtgcttctt gccggcggca gaggcgcccg catgggggcg caggtcccca aacaggaatt
     1921 ggaactcgac ggcaggaaga tataccgcat cgccgccgaa cggttcgcct ccttcaccat
     1981 cgttgatgct atcatgttcg tctgccccgc cgaacggctg gacgcataca aggccgggct
     2041 tgcgggcatc ccgaaggtcc gttgggtcat cgcgggtggt gccgagcgac acgattcggt
     2101 ctggaacgct cttgaggcgc tcaggaacga cccgcctgcg gtggtgctga tgcacgacgc
     2161 ggtgcgcccc tttgtgagcg gacggacggt ggccgaagtg gcccgtgtcg ccgccgaaca
     2221 cggcgcggcg acagcggcgg cccgcgcggt cgacacgctt tacgaggtgc gcgacggca
//
LOCUS       DAOQUE010000455         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_59245_length_2268_cov_4.015816, whole genome shotgun sequence.
ACCESSION   DAOQUE010000455 DAOQUE010000000
VERSION     DAOQUE010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59245_length_2268_cov_4.015816"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PKH10_11750"
     CDS             complement(<1..936)
                     /locus_tag="PKH10_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28834.1"
                     /translation="MIERMRKILILGPRPHQEELLRHLQDAGIVQVEAYHGDAYPIDG
                     RHVTPLGADLALSAYRTLQKYERQMRIDKVTAPTERSGLPPERIVEELPLLEDRVRIL
                     RDKIQQLSNKLDSIEPWGIFDLAMLRDICEKGDLTIQLWEVAHDYADDVSIDGAIAVM
                     EVNVDPERHYFLTFSDHTLHFTDCLEIRYDEDHPTLARRIEALKRELAQTYETIHRMV
                     NDRDDVFRHYLRELDKFHYGSAAWGVIRLMDDALFVLQAWCPDRSLPRLSEIAKEIPI
                     QIEEVDPDERERVPTLMVNEGSAALGEDLVKIYDTP"
     gene            complement(933..1568)
                     /locus_tag="PKH10_11755"
     CDS             complement(933..1568)
                     /locus_tag="PKH10_11755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNT28835.1"
                     /translation="MAQIKLNKTELKNQKNLLDQLTKYLPTLQLKKQQLQAEVETSRA
                     QIQRAEEDLAARRSALAKWSILFSQRLTVDMFDYLEIDAVTRDKENIAGIDVPVFTGI
                     TFRTRPYSFFVMPVWLDRALKELRELIALREKVKIMREKQALLAEELRQTNIKVNLFE
                     KRMIPDCRENIRRIKIFLGDQEIAAICNAKIAKDKLKRRDSSERREEVAAA"
     gene            complement(1578..>2268)
                     /locus_tag="PKH10_11760"
     CDS             complement(1578..>2268)
                     /locus_tag="PKH10_11760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011457880.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNT28836.1"
                     /translation="DYPVVVPHVENDLALAVAEQFSVQNKNVLCLLTDMTAFSDALKE
                     ISISMEKVPSNRGYPGSLYSDLAARYEKAVDIEGSGSITIIAVTTMPGNDVTHPVPDN
                     TGYITEGQFYLHGGIINPFGSLSRLKQQVMGKVTRKDHSDLANAMIRLYADAKKARDR
                     ESMGFRLSNWDQKLLAYAKKFETRMMDLEVNLDIEQQLDLGWTILAECFEKHEVGLKG
                     EWITKYGKWGK"
BASE COUNT          400 a          633 c          711 g          524 t
ORIGIN      
        1 cggcgtgtcg tatatcttca cgagatcctc gccgagcgca gcggagccct cgttgaccat
       61 cagggtcggc acccgctccc gttcgtcggg atcgacctct tcgatctgta tcggtatctc
      121 cttggctatc tcggagagtc ggggtaacga gcggtcgggg caccaggcct gcaggacgaa
      181 cagcgcgtcg tccatcaggc ggatgacgcc ccacgcggcg gaaccgtagt gaaacttgtc
      241 gagctcgcgc aggtagtgac ggaacacatc gtcacggtcg ttcaccatgc ggtgaatggt
      301 ctcataggtt tgggccaatt cccttttgag cgcctcgatg cggcgcgcga gggtcggatg
      361 gtcctcgtca tagcgtatct cgaggcaatc ggtgaaatga agcgtgtggt cagaaaaagt
      421 gaggaaataa tgtcgttcgg ggtccacatt cacctccatg acggcgatgg ccccatcgat
      481 ggaaacgtcg tcggcgtagt cgtgggcgac ctcccagagc tggatggtga gatccccctt
      541 ctcgcagata tcgcggagca tcgcgagatc gaagatgccc cacggctcga tggaatcgag
      601 tttattcgac agttgctgta tcttgtcgcg caggatacgg acccggtcct cgaggagcgg
      661 tagttcctcg acgatccgtt ccggcggcag gccggaccgt tcggtcggtg cggtcacttt
      721 atcgatacgc atctgccgtt cgtatttctg gagcgtacgg taggccgaca gcgcgaggtc
      781 ggcgccgagc ggggtcacat gacggccgtc gatgggatag gcgtcgccat ggtaggcctc
      841 cacctgcacg atgcccgcat cctgcagatg gcgcaggagt tcttcctgat gcggccgcgg
      901 cccgaggatg agtatcttgc gcatccgttc tatcatgccg ctgccacctc ttcacgccgt
      961 tccgatgagt ccctgcgctt gagtttatct ttggcgatct tcgcgttaca gatggccgcg
     1021 atctcctgat cgccgaggaa tatcttgatg cgccggatgt tctcacggca gtcggggatc
     1081 atccgttttt caaagaggtt cactttgatg ttcgtctggc gcagttcctc ggcgaggagc
     1141 gcctgtttct cgcgcatgat cttcactttc tcgcggagcg caatgagttc tctcaattcc
     1201 ttgagcgcgc ggtcgagcca gaccggcatg acgaagaaac tgtaagggcg ggtccggaaa
     1261 gtgatgccgg tgaagaccgg cacatcgatg ccggcgatgt tctccttgtc gcgggtcacc
     1321 gcgtcgatct caaggtagtc gaacatgtcg acggtgagcc gctgggaaaa aaggatggac
     1381 cactttgcaa gcgctgagcg gcgcgccgcg aggtcctcct cggcgcgctg tatctgggca
     1441 cgcgaggtct cgacctcggc ctgcaactgc tgtttcttga gttgcagggt gggcaggtac
     1501 ttggtcaact ggtcgagcag gttcttttga ttcttgagct cggtcttgtt gagctttatc
     1561 tgcgccatcg tcggacccta tttaccccat ttgccgtact tggttatcca ttcccccttg
     1621 agaccgacct cgtgcttctc gaagcattcg gcgagtatcg tccagccgag gtccagttgc
     1681 tgttcgatgt cgaggttgac ctcgagatcc atcatccggg tctcgaactt tttcgcgtag
     1741 gcgagaagtt tctggtccca gttcgagagg cggaatccca tcgattcacg gtcgcgcgcc
     1801 tttttcgcgt cggcgtaaag ccgtatcatg gcgttggcga ggtcggaatg gtccttgcgg
     1861 gtgaccttgc ccatgacctg ctgtttgagc cgcgagagcg atccgaaggg gttgatgatg
     1921 ccgccgtgca ggtagaactg accttcggtg atgtagccgg tgttgtcagg gaccgggtgg
     1981 gtgacatcgt tgcccggcat cgtcgtgacg gcgatgatgg tgatcgaccc gcttccctcg
     2041 atgtcgaccg ccttctcata acgggcggcg agatcggaat aaagcgagcc gggatacccg
     2101 cggttcgacg ggaccttttc catcgatatc gatatctcct tgagcgcgtc cgaaaaggcg
     2161 gtcatgtcgg tcagcaggca caggacattc ttgttctgca ccgagaactg ttcggcgacc
     2221 gcgagcgcga ggtcgttctc aacgtggggc acaacgacgg ggtaatcg
//
LOCUS       DAOQUE010000456         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_59255_length_2268_cov_3.046091, whole genome shotgun sequence.
ACCESSION   DAOQUE010000456 DAOQUE010000000
VERSION     DAOQUE010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59255_length_2268_cov_3.046091"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="PKH10_11765"
     CDS             complement(<1..1365)
                     /locus_tag="PKH10_11765"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28837.1"
                     /translation="MRKKTGEFSRRWEYLKNRYYQWIKQSDRKTSIAILLSLSVVAAY
                     LTIPDTALSFLPTATSDIGKVMKFSVRADRDYEVADTEQTEKKQAEALRKVLRHYTYQ
                     DRAATYQKMRKAFAAMRQKAVGVLIEKGLPGGIPAPQEWERLNRAALEVNLPLIVPEN
                     LRGTVYLALDGHRKDFEKQLGVAIDSDLFAALRAGFFSLDIEEAVFAIIERLDNFYIL
                     RSGPGRDYHPDRIAVRKGSNLINRPLHRVITNLSLPAEIANTQRLLRQENELSAKGMA
                     LIAATAHALITDNLVPDEESSQKAREETMAATPPVLIKVKNGELIARTGDTVTRQHIA
                     VFREIAEKKKDQPTALLFIEHLLFIAFTLSVGFLSFRRSISKFSTQNKDLLLMALLAT
                     VSIGFTYGIAAISVPFSQWIGNIDPRIFNFLLPFPFVAATVRLLINSETALFFIVSIM
                     LLFAN"
     gene            complement(1374..1991)
                     /locus_tag="PKH10_11770"
     CDS             complement(1374..1991)
                     /locus_tag="PKH10_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNT28838.1"
                     /translation="MKPLTPGQNELVEAVDRHDIVFVDGPAGTGKTFLAAALAVKFLR
                     EGRIERVVLTRPAVEAGEKLGFLPGSLEEKVDPYLRPLFDAIETLFTFEESARLISNR
                     QIEVIPLAFMRGRTLKKSFVVLDEAQNTTVPQMKMFLTRFGDKSRMVVAGDSSQRDIE
                     HRDNGFTDAMRRLSMVPGVAVTRLTASDIVRADIIGRIIEAYEKA"
     gene            complement(2032..>2268)
                     /locus_tag="PKH10_11775"
     CDS             complement(2032..>2268)
                     /locus_tag="PKH10_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNT28839.1"
                     /translation="PDRRTMWHAQTPQGFRYDILRRAHEEFRKKGGATTDDTRLVQAI
                     GQMVRVVEEPLTNLKITGPADIAIATALYRELKE"
BASE COUNT          394 a          623 c          757 g          494 t
ORIGIN      
        1 attggcgaac aggagcatga tcgagacgat gaagaagagg gctgtttcgc tgttgatgag
       61 cagccgtacc gttgctgcga cgaaggggaa ggggagcagg aagttgaaaa tgcgcggatc
      121 tatgttgccg atccactgcg agaaggggac cgatatcgcc gcgatgccat aggtgaaacc
      181 gatggagacc gttgcgagga gcgccatcag caggagatcc ttgttctgcg tgctgaattt
      241 gctgatgctt cgccggaagg ataggaaccc gacggagagg gtgaacgcga tgaagagcag
      301 gtgttcgatg aacaggagcg cggtcggttg atcctttttc ttttcggcta tctcgcggaa
      361 tacggcgatg tgctggcggg tcaccgtatc gccggtccga gcgatgagtt cgccgttctt
      421 tacctttata agcaccggtg gcgtggcggc catcgtttct tcgcgggcct tttgagaact
      481 ctcctcgtcc ggcacaagat tatcggtgat gagtgcgtgg gcggtcgccg cgatgagcgc
      541 catgcccttg gcggagagtt cgttctcctg ccgaagcagg cgctgggtgt tggctatctc
      601 ggccggaagg gacaggttgg tgatgacgcg atggagcgga cggttgatga gattgctccc
      661 cttgcgaacc gctatccgat cgggatggta gtccctgccc gggccggagc ggaggatgta
      721 gaaattgtca agtcgctcta tgatggcgaa taccgcctcc tctatgtcga gagagaagaa
      781 tccggcgcgt aaagcggcga agaggtcgct gtcgatggcc accccgagct gtttttcgaa
      841 atccttccgg tgaccatcga gggcgagata gacggtgccg cgaaggttct ccggtacgat
      901 caggggcagg ttcacctcga gagcggcgcg gttgagccgt tcccattcct gaggtgccgg
      961 tatcccgccg gggaggccct tctcgatgag gacgccgacc gctttctgtc gcatggcggc
     1021 gaaggctttg cgcatcttct ggtaagtcgc cgcgcggtcc tggtaggtgt agtggcggag
     1081 caccttgcga agcgcttccg cctgtttctt ttcggtctgt tcggtatccg ccacttcgta
     1141 atcgcggtcg gcgcgcacgc tgaatttcat gaccttgccg atgtcggagg tggcggtcgg
     1201 caggaaactc aacgcggtgt cggggatcgt caggtaggcc gcgacgaccg agagggagag
     1261 gagtatggcg atcgaggtct tacgatcgct ttgtttgatc cactgatagt aacggttctt
     1321 gagatattcc caacgccggg agaattcacc ggtcttcttg cgcatctcgg tcatcatgct
     1381 ttttcgtatg cctcaatgat cctgccgatg atgtcggctc tcacgatgtc gctcgcggtc
     1441 agccgggtga ccgcgacccc gggcaccatc gagagtcgcc gcatggcgtc ggtgaagccg
     1501 ttgtcgcggt gttcaatgtc gcgctgagag gagtcaccgg cgaccaccat gcggcttttg
     1561 tcgccgaagc gggtcaggaa catcttcatc tgcggcaccg tggtgttctg ggcctcgtca
     1621 aggaccacaa aactcttttt cagcgttctg ccgcgcatga aggcgagcgg tatcacctct
     1681 atctggcggt tcgatatgag tcgcgccgat tcctcgaagg tgaacagggt ctcgatggcg
     1741 tcgaagagcg ggcgcaggta ggggtcgacc ttctcttcga gcgaaccggg gaggaacccc
     1801 agtttttcgc ccgcctcgac cgccggacgg gtcagcacca cccgctcgat gcgtccctcg
     1861 cgcaggaact tcacggcgag cgcggcggcg aggaaggtct tgccggtgcc ggccgggccg
     1921 tccacgaaca cgatgtcgtg gcgatcgacc gcctcgacca actcgttctg gcccggggtc
     1981 agcggcttca cggccgtccg gtccttgtcg cgcaggatga tgtctatggc gtcactcctt
     2041 cagttctcgg tacagggcgg tggcgatggc gatatcggcc ggccctgtta tcttgagatt
     2101 ggtaagcggc tcctcgacga cgcgtaccat ctggccgatc gcctgcacga gtcgtgtgtc
     2161 atcggtcgtc gcgccgccct tctttcggaa ctcttcgtgg gcacggcgca ggatatcgta
     2221 ccggaacccc tgcggggtct gggcatgcca catcgtgcgg cgatcggg
//
LOCUS       DAOQUE010000457         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_59589_length_2260_cov_4.112925, whole genome shotgun sequence.
ACCESSION   DAOQUE010000457 DAOQUE010000000
VERSION     DAOQUE010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59589_length_2260_cov_4.112925"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PKH10_11780"
     CDS             complement(<1..852)
                     /locus_tag="PKH10_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNT28840.1"
                     /translation="MRRIVVLLLLFCAPSLFAALDDNIFYTFYSQKSARSVEALAFVR
                     ELATQNREVTFKEVEVNASPEDTALFREALDEFELEKPLVPFFTLGGRHAVGWEPNDP
                     AFREKVTGLVSRYRETAGDGTFYFFYSYKCPHCRKARPFMNEWERRYPQIHFKWLEII
                     RNDENMALFRRKAELLGIENAGTPTFVLGDQYVGGFEPGTQDIEVDEMIQRYLSGDKR
                     DRGPVTTVNIPFIGTLDARVVSLPSFTFLIGLLDGINPCAMWVLMFLLTLLVNTKSRG
                     MLLMIGSV"
     gene            complement(866..>2260)
                     /locus_tag="PKH10_11785"
     CDS             complement(866..>2260)
                     /locus_tag="PKH10_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HNT28841.1"
                     /translation="DYHAARVLPGFDPNVKMRLLQKLKDDAELIMCIYAGDIEKKKMR
                     ADFGITYDADALRTIDDYREWGLTVRAVVITRYENQPSAAAFKSKLERRGVTVYTHSF
                     TKGYPTDVDMIVSPEGYGANPYIETHKPIVVVTGPGPGSGKLATCLSQLYHEHLRGVN
                     AGYAKFETFPIWNLPLKHLVNVAYESATCELRDVNVIDHFHLEAHKEYSVNYNRDVEA
                     FPLLKRIIEKITGEPSHYKSPTDMGVNRAGFGIVDDAAVTEASRQEIIRRYYRYSCEY
                     ALGLVNRETVERAEVIMKDVGAAPTDRSVVIPAQKAAGEAKEKSKGNEGIFCGAAIEL
                     GDGTIITGNNSPLMHAASALILNAVKHLARIPKEIDLLTPTITGSIASLKKEIYCGRA
                     ASLDLAETLIALSISATFNPSAAVALEKLKDLRGCEVHLTHMPSPGDEAGLRKLGVNV
                     TSNPEFASKYLFSS"
BASE COUNT          428 a          663 c          699 g          470 t
ORIGIN      
        1 gacgctcccg atcatcagga gcatcccgcg gctcttggtg ttcaccagca gggtcagcag
       61 gaacatgagc acccacatcg cgcaggggtt gatgccgtcg aggagcccga tgagaaaggt
      121 gaaactcggc agggagacga cccgtgcgtc gagcgtcccg atgaaaggga tattgaccgt
      181 cgtcaccgga ccgcgatccc gcttatcgcc cgagagatag cgctgtatca tctcgtcgac
      241 ctcgatatcc tgcgttcccg gctcaaaacc gcccacatac tggtcgccga gcacaaaggt
      301 cggcgtaccg gcgttctcga tgccgaggag ctccgccttg cgtctgaaga gcgccatatt
      361 ctcatcgttg cggatgatct caagccattt gaaatgtatc tgcgggtaac gacgctccca
      421 ttcgttcatg aaagggcgcg ccttgcgaca atgaggacat ttgtaggaat agaagaaata
      481 gaaggtgccg tcgccggcgg tctcgcgata tcgggaaacg agtccggtca ccttctcacg
      541 gaaggcgggg tcgttcggtt cccagccgac cgcatgccgt ccaccaagcg tgaagaacgg
      601 tacgagcggt ttttcgagtt cgaattcatc gagcgcctcg cggaagagcg ccgtgtcctc
      661 cggcgaagca ttcacctcga cctccttgaa ggtcacttcg cggttctgcg tcgccaattc
      721 acggacgaag gcgagcgcct cgaccgaccg ggcgctcttt tgcgaataga aggtgtagaa
      781 aatgttgtcg tcgagcgccg cgaaaagaga gggcgcgcaa aaaagaagga gcagaacgac
      841 gatccgcctc atcgcgcaga ccctcctatg aactgaacag atatttcgag gcgaattcgg
      901 gattgctggt cacattgacg ccgagtttgc gcagacccgc ctcgtctccg gggctcggca
      961 tgtgtgtcag atggacctca cagccgcgca ggtccttgag tttctcgagc gcgaccgccg
     1021 ccgacgggtt gaaggtggcg cttatcgaga gcgcgatcag cgtctcggca aggtcgagac
     1081 tggccgcacg gccgcagtat atctcctttt tcagcgacgc gatggagccg gttatcgtcg
     1141 gggtgagcag atctatctcc ttcgggatgc gcgccagatg cttcacggcg ttcaggatga
     1201 gcgccgaagc ggcatgcatg agcggcgaat tgttgccggt gatgatcgtg ccgtcgccga
     1261 gttctatcgc cgcgccgcag aatatcccct cgttcccctt gctcttctcc ttcgcttccc
     1321 cggccgcctt ctgcgccggg atgacgaccg accggtcggt cggcgccgca ccgacatcct
     1381 tcatgatgac ctcggcccgt tccaccgttt cacggttgac gagccccagc gcgtattcgc
     1441 aggaatagcg atagtaacga cggattatct cctgtcgcga tgcctcggtg accgccgcat
     1501 cgtccacgat gccgaacccg gcgcggttga cccccatatc ggtcggcgac ttgtaatgcg
     1561 acggctcgcc ggtgatcttc tcgatgatac gcttgaggag cgggaacgcc tcgacatcac
     1621 ggttgtagtt gaccgagtat tccttgtgcg cctccagatg gaaatggtcg atgacattga
     1681 catcgcgcag ttcgcaggtg gccgattcgt aggcgacatt gaccagatgt ttgagcggca
     1741 gattccatat cgggaaggtc tcgaacttcg cgtagccggc attcaccccg cgcaggtgct
     1801 cgtggtagag ttgcgaaaga caggtggcca gtttcccgct gccgggaccg gggccggtca
     1861 ccacgacgat gggtttatgc gtctcgatat agggattcgc gccgtaccct tcggggctga
     1921 cgatcatgtc gacatcggtc gggtagccct tggtgaaact gtgggtgtag accgtgacac
     1981 cgcgccgttc cagtttgctt ttgaaggcgg cggccgacgg ctggttctcg tagcgggtga
     2041 tgaccacggc gcgcaccgtg agcccccatt cgcggtagtc gtctatcgtc cgcagagcgt
     2101 cggcgtcgta ggtgatgccg aagtcggccc gcatcttctt cttctcgatg tcgccggcgt
     2161 agatgcacat gatgagttcg gcgtcgtcct tgagtttctg gagaagccgc atcttgacat
     2221 tgggatcgaa cccgggcagg acgcgggccg cgtggtagtc
//
LOCUS       DAOQUE010000458         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_59596_length_2260_cov_3.417234, whole genome shotgun sequence.
ACCESSION   DAOQUE010000458 DAOQUE010000000
VERSION     DAOQUE010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59596_length_2260_cov_3.417234"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKH10_11790"
     CDS             <1..217
                     /locus_tag="PKH10_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28842.1"
                     /translation="ANKGGKDIFLTAVSANGAEEWTTLYGTPEDDGALSMCLFDGWAV
                     IAGWTAGTLGEKSAGDHDAVVIYHPVE"
     gene            315..1115
                     /locus_tag="PKH10_11795"
     CDS             315..1115
                     /locus_tag="PKH10_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28843.1"
                     /translation="MRKAVWSIWAVLVLFSCSNGDPVPFVTPDNGSGDGDNDVVVETD
                     PAPDDVEGEGTPDDDAPVVESDPGVQDPDQPEGEIPDDGVIGKNCKVDGDCGAPLICV
                     NAKCAEGCAGDGDCGAYPNTVCNKKLARCVNATASDGACDETNCPVGCCYAEKGFLGL
                     KCAETASFSVCGLCPQGEIYMDGKQCVPAACKTADTKCSVYNSSSPRADCFKCMSEKD
                     YLCVDDPQCNPGSTMLMINVMECVPAGERCQRRDICCSGMPCIQGYCY"
     gene            complement(1109..2218)
                     /gene="lnt"
                     /locus_tag="PKH10_11800"
     CDS             complement(1109..2218)
                     /gene="lnt"
                     /locus_tag="PKH10_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNT28844.1"
                     /translation="MQVAHPGALAREDLGVGPALPKDLPEDRPLVRRERRVHETVACL
                     VFLIVMGAYGLWRLDAVRSAEPVRQVKVALLQPNIGQEIINAKTDQLPIIVDLFTRLL
                     KQIPPDTDLVVWHEAAMPVRIPYGFTDFNRLWKKFFPDAPQFPKQIIGIDLVNASDPS
                     RYKYYNAAGFIDNGRIARIYEKVKLAPFGEYLPWSDLMNSLGLTTLVPSTVGAFERGT
                     EYTVYDFGFAKASILICYDGTFSENVREFVLNGSDLIVSITNDAWFGYSSAAAQHMSF
                     YNFRAVETGRTIVRAANIGISGVVLPDGTMPVRTPIFTERLLVEKVPIYHLEPPYLRW
                     GNFFLWTVVGGSTLGLLRAIVLNRRRGKAAKKVRQ"
BASE COUNT          451 a          605 c          743 g          461 t
ORIGIN      
        1 cgccaataag ggagggaaag acatctttct gacagcggtc tcggcgaatg gcgcggaaga
       61 atggacgacg ctttatggta cgccggagga cgatggtgcc ctgtcaatgt gtcttttcga
      121 cggttgggcc gttatcgcag gatggaccgc aggtacacta ggggaaaaaa gcgcaggcga
      181 tcacgatgcg gtcgtgatat atcacccggt ggaataaaat agggttcccg tccgaaactt
      241 ctttactcgc cgaacggtct atccgattca gagtttggtt agttccataa aggttttttt
      301 gaaggaggac gatcatgaga aaagcggtgt ggtctatctg ggcggtgctg gtgttattct
      361 cctgctcgaa cggagacccc gttcccttcg ttacaccgga taacggctcc ggtgacggcg
      421 ataatgatgt ggtggtcgag accgatcctg ctcccgacga tgttgagggt gaagggacac
      481 ccgatgacga tgcccccgtg gtcgagagcg atcccggcgt gcaggatcct gatcagcccg
      541 aaggggagat cccggacgat ggcgtgatag ggaagaactg caaggtggac ggcgactgcg
      601 gtgcgccgct catctgtgtc aacgcgaaat gtgccgaagg atgcgcgggt gacggtgatt
      661 gtggcgccta tccgaacact gtctgcaaca agaaactggc tcggtgcgtc aatgcgacgg
      721 cctcggacgg cgcctgtgat gagaccaact gccccgtcgg ctgttgttac gccgaaaagg
      781 gtttcctcgg gctcaaatgt gccgagaccg catcgttcag tgtctgcggt ctctgtccgc
      841 agggcgagat atatatggat ggcaaacagt gcgtacctgc cgcctgcaag acggccgaca
      901 cgaaatgttc ggtctacaat tcatccagcc cgcgcgccga ctgtttcaag tgcatgagcg
      961 aaaaagacta cctgtgcgtc gatgatcccc agtgcaatcc cggttcgacg atgctcatga
     1021 taaatgtcat ggagtgcgta ccggccggcg agcgctgtca gcggcgcgat atctgctgca
     1081 gcggcatgcc gtgcatacag gggtactgtt actgacggac cttcttcgcc gcttttccgc
     1141 gacgccggtt caacacgatg gcccgtagca accccaaggt gctccctccg accaccgtcc
     1201 agaggaagaa gttcccccag cgcaggtacg gcggctcaag gtggtatatc ggcacctttt
     1261 cgacgagtag ccgttcggtg aatatcggcg tgcggaccgg catggtgccg tcgggaagga
     1321 cgacccccga tatgccgatg ttggcggcac ggacgatggt gcgccccgtc tcgaccgccc
     1381 ggaagttata gaaactcatg tgctgggcgg cggcggaact gtagccgaac caggcgtcgt
     1441 tggttatgct gactatcagg tcggacccgt tcaggacgaa ttcgcgcaca ttctccgaga
     1501 aggtgccgtc atagcagatg aggatggagg ccttcgcaaa gccgaaatca tagaccgtgt
     1561 attcggtgcc gcgctcgaag gcgccgacgg tgctcgggac gagcgtggtg agtcccagcg
     1621 aattcatgag atcgctccat ggcaggtatt cgccgaacgg ggcgagtttg accttttcgt
     1681 agatgcgggc gatccgaccg ttatcgatga acccggcggc gttgtagtat ttgtagcgcg
     1741 aaggatcgct cgcgttcacg aggtcgatac cgatgatctg tttcgggaac tgcggtgcgt
     1801 cggggaagaa tttcttccag agccggttga aatcggtgaa gccgtagggt atgcgcaccg
     1861 gcatcgccgc ctcgtgccag acgaccagat cggtgtcggg cggtatctgt ttcaagagtc
     1921 gcgtgaagag atcgacgatg atgggcaact ggtcggtctt cgcgtttatg atctcctgtc
     1981 cgatgttcgg ttgaaggagc gcgaccttca cttggcgcac cggctcggcg gaacgcactg
     2041 cgtcgagccg ccacaggccg taggcgccca tgacgatgag gaatacgagg caggcgaccg
     2101 tttcatggac gcgccgctca cggcggacga gggggcgatc ctcgggaaga tctttgggca
     2161 gtgccgggcc gacgccgagg tcttcgcggg ccagagcgcc cgggtgcgcg acctgcacgt
     2221 gcgccaggcc gtggtcgggc gctggctctt catggtgctg
//
LOCUS       DAOQUE010000459         2246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_60196_length_2246_cov_4.748973, whole genome shotgun sequence.
ACCESSION   DAOQUE010000459 DAOQUE010000000
VERSION     DAOQUE010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60196_length_2246_cov_4.748973"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PKH10_11805"
     CDS             complement(<1..633)
                     /locus_tag="PKH10_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT28845.1"
                     /translation="MRRSDRLIHAILVALCATAILAGCAGTPKDERARAEQALRDAEV
                     VKNCDPETYATALELFNAAQAAEKDKDYTKAKELYLAAEDKVKKFAAYYRANPDKCLA
                     QKKKEKKDDDTGAIVELDPDSPDNPDMRLPVVYFALDSYDLRPDDAEKLKNVAQWMGR
                     FGTVAVRIEGHADERGSTDYNLSLGERRAQEVLRFLQRQGIDAGRVRTLSY"
     gene            complement(630..1220)
                     /locus_tag="PKH10_11810"
     CDS             complement(630..1220)
                     /locus_tag="PKH10_11810"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT28846.1"
                     /translation="MKATIQIITKHGLKELTSKRIAKAVGISDAAIYRHFPNKEAILM
                     EVLQLFSDRVATIHKTILTADEPVFRKLELLFQEKSRRLVEHPEQTLLMRSAMVFRGH
                     PSLRRSAVEMLDAYKADVISVLRQGQASGVIDPRIDPEHLYHCIIGGLYFLTERWETS
                     QRSFDLPEATAKLWNSIQHMLSNKNTEKNLPDGGPS"
     gene            complement(1367..1741)
                     /locus_tag="PKH10_11815"
     CDS             complement(1367..1741)
                     /locus_tag="PKH10_11815"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT28847.1"
                     /translation="MKERRSGERFKLSGTAEIAREGKKKSKGELRDLSINGAFIAINN
                     GFEPYEDIEIAITVRNDRKSLAATLKGVVSRKDGDGIGVQFTGMDMNSFRVIRDIAKL
                     YAEDPKKIEKDIKRLSDTPVTR"
     gene            complement(1773..>2246)
                     /gene="pyrE"
                     /locus_tag="PKH10_11820"
     CDS             complement(1773..>2246)
                     /gene="pyrE"
                     /locus_tag="PKH10_11820"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNT28848.1"
                     /translation="GPAYKGIPLAVAAASALHTDHKRDVAFCFNRKEAKDHGEGGTLI
                     GHKLAAGDRVLIIEDVVTAGTSVAETVPILTAAAPVKLAGLVVSVDRQERGKGTRTAL
                     AEIRDTYGMDTFAIVTMSEVVAYLHGREVNGAVVLTDEIKKRIGEYYAQYGSTEG"
BASE COUNT          387 a          673 c          656 g          530 t
ORIGIN      
        1 gtacgagagg gtgcgcaccc gtcccgcgtc tatcccctgt cgctgaagga accggagcac
       61 ctcctgcgcg cgccgttcgc cgagcgacag gttgtagtcg gtcgagccgc gctcgtcggc
      121 gtggccctct atccgcaccg caacggtgcc gaacctgccc atccattggg caacattctt
      181 gagtttctcg gcatcgtcgg gacggaggtc gtaactgtcg agagcgaaat agaccaccgg
      241 cagccgcatg tcgggattat cgggcgaatc ggggtcaagt tcgacgatcg cgccggtatc
      301 gtcgtccttt ttctcctttt ttttctgcgc gagacatttg tcggggttgg cacggtagta
      361 ggcggcgaat ttcttcacct tgtcctcggc ggcgagatag agttctttcg ccttcgtgta
      421 gtccttgtcc ttttcggccg cctgtgcggc gttgaacagt tcgagcgcgg tcgcgtaggt
      481 ctcggggtcg cagttcttca cgacctcggc gtcgcggagc gcctgttcgg cgcgcgcccg
      541 ttcatccttc ggggtgccgg cgcaaccggc caggatggcg gtagcgcaga gggcgacaag
      601 gatcgcatgg atcaatcggt ccgatcgtct catgatgggc ctccatcggg caggtttttt
      661 tcggtgttct tattcgacaa catatgctgt atgctgttcc agagttttgc ggttgcttcg
      721 ggcagatcga aactccgctg agaggtctcc catcgttccg tcaaaaagta aaggccgccg
      781 atgatacagt gatagagatg ttcaggatcg atccgtggat cgataacgcc cgaggcctgc
      841 ccctgacgaa ggacggagat cacatcggcc ttgtatgcat cgagcatttc caccgcgctc
      901 cggcgcaggg aggggtgtcc ccggaatacc atggcggagc gcatgagaag cgtctgttct
      961 ggatgttcga ccagacgacg acttttttcc tgaaagagca gttccaactt tctgaacacc
     1021 ggctcgtcag cggtgagaat ggttttatgt atggtcgcca cacggtcgct gaaaagttgc
     1081 aacacctcca tcaggatagc ctccttgttc ggaaagtgac gatagatcgc agcatcggat
     1141 atcccgaccg ccttggcgat acgcttgctg gtcaattcct tcaggccatg cttggttatg
     1201 atctgtatcg tcgctttcac gatctgctgg cgccgagttc cccgatcttt cctgatcatg
     1261 ccgtacacct caaatgctag tgagcactca ctaacatatg atgcagaaac aaaagacaaa
     1321 tgtcaataaa aataaaaagg ttatttgatg tcgggatgat ggtgtgttaa cgggtgaccg
     1381 gggtgtcgct caggcgtttg atgtcctttt ctatcttctt cggatcctcg gcgtagagtt
     1441 tcgcgatgtc gcggatgacg cggaaggagt tcatgtccat cccggtgaac tgcacgccga
     1501 tgccgtcgcc gtccttgcgg ctcaccaccc ccttgagcgt ggcggcgagg ctcttgcggt
     1561 cgttgcggac cgttatcgct atctcgatgt cctcatacgg ttcgaagccg ttgttgatgg
     1621 cgatgaaggc gccgttgatg gacaggtcgc gcagttcccc cttgctcttt ttcttgccct
     1681 cccgcgctat ctcggccgtg ccgctcagtt tgaaccgttc gccgctccgc cgctccttca
     1741 tgagatcctc cagaaagaaa acgagctccc ctctaccctt cggtgctgcc gtactgcgca
     1801 taatactctc ctatccgctt ctttatctcg tcggtgagaa cgaccgcgcc gttcacctcg
     1861 cggccgtgca ggtacgcgac cacctcgctc atcgtgacga tggcgaaggt gtccatgccg
     1921 taggtgtcgc gtatctcggc gagcgccgtg cgggtccctt tgccgcgctc ctgcctatcg
     1981 accgacacca cgagtcccgc caacttcacc ggcgccgccg cggtgagtat cggcaccgtc
     2041 tcggcgaccg atgtcccggc ggtcaccaca tcctcgatga tgagcacgcg gtcgcccgcc
     2101 gcgagtttgt gaccgatgag cgtgcccccc tcgccgtggt ccttcgcctc cttgcggttg
     2161 aagcagaacg cgacatcgcg cttatggtcg gtgtggagcg ccgacgccgc cgcgaccgcg
     2221 agcgggatgc ccttgtacgc ggggcc
//
LOCUS       DAOQUE010000460         2243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_60324_length_2243_cov_4.762340, whole genome shotgun sequence.
ACCESSION   DAOQUE010000460 DAOQUE010000000
VERSION     DAOQUE010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60324_length_2243_cov_4.762340"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="PKH10_11825"
     CDS             <1..1141
                     /locus_tag="PKH10_11825"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNT28849.1"
                     /translation="PGGLILAALSGVVFTALVQSSTATAAIVIAGAASGLFSLHLSCA
                     LMLGATLGTTITALLASVNAGSAGRRTAMMHFALNVVGFFGGLLVFYPLVHLITTTTA
                     AAGIGVAVATYMTLHKVLLTAGIFPFRRRFAEWIARLVPDRFPGVHERLSVRPLAHDA
                     TDEAVLEEFRRDIALFMRYLRDLFAFSIVVSLKRKESPLFAKIEQYEQIIDQGHKKLV
                     RLIHEQSHRNRQDLWLYLKMSDEAESMADHLRSIAKYGIRLRERMKDPQALERLLIHT
                     AHRRVFTLFHRVCLCGHFHAGDLAACDEAEREIRRHKRELMLTITEARAPLDPDAILE
                     LADILSEHSKINHSVKRVLQANLDHLEGRGVFRYRHEKISCPPAG"
     gene            complement(1119..2231)
                     /locus_tag="PKH10_11830"
     CDS             complement(1119..2231)
                     /locus_tag="PKH10_11830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HNT28850.1"
                     /translation="MREIRELDDLPADTTAVFLFSAMTADLDRSVAEMRHLRGRFPNA
                     RFIIGGPHASARPREVIERGFDLAAVGEGEEMIREIAERVREGRSLEGVRGLCRLEGD
                     ALVGAPAAPVDIERFPMMAHRVKLYGPIEITRGCASVCRYCQTSFFHGVAERWRSREA
                     ILAAVRELAAREFAYYRFLSPNALGWHNPDGRTPEPLHELLAAVRDTVGPRRKVIFGT
                     FPAEIRPEFATRESMAVIKRYCDNPIIVVGGQSGSDRMLEHMHRGHTVADIYRAVENT
                     KAAGLHPIVDLIFGLPGETPDDLALTVALMERLLLQGAHIRGHIFMPLPGTPWEHERP
                     GAVPDELARRFKWMSSRGMLSGCWEEQSVLNRRAGN"
BASE COUNT          406 a          774 c          658 g          405 t
ORIGIN      
        1 acccggcgga cttatccttg cggctctttc gggcgtagta ttcaccgcgc ttgtccagtc
       61 gagcaccgcc actgccgcca tcgtgatcgc cggcgccgcg agcggtcttt tctccctgca
      121 cctttcctgt gcccttatgc tcggagcaac gctcggcacc accatcacgg cgctcctcgc
      181 atcggtcaac gcaggaagcg caggcaggcg gaccgccatg atgcatttcg ccctcaatgt
      241 cgtgggattc ttcggcgggc tgttggtatt ctaccccttg gtgcacctta tcacgacaac
      301 tactgcagca gccggcatcg gcgtggccgt ggccacctat atgacgcttc acaaggtgct
      361 gttgaccgcc ggcattttcc cgttccgccg tcgtttcgcc gagtggatag ctcgactcgt
      421 ccccgatcgg ttccccggcg tccatgaaag gctttcggtc cgcccgctcg cgcacgacgc
      481 gaccgatgag gcggtgcttg aagagttccg acgcgacatc gccctgttca tgcgctacct
      541 ccgcgacctg ttcgcctttt ccatcgtcgt ctcgctgaaa cggaaggaat cgcctctgtt
      601 cgccaagata gagcagtacg agcagatcat cgatcaggga cacaagaaac tggtgcgcct
      661 catccatgaa caaagccacc gcaaccgcca ggatctctgg ctgtatctca agatgtccga
      721 cgaggccgaa tcgatggccg accacctgag atcaatagcc aaatacggca tccgcctgcg
      781 ggagaggatg aaagatcctc aggccctcga acggcttctc atccacaccg cgcaccgccg
      841 cgtcttcacg ctgttccatc gcgtctgcct ctgcggccat tttcacgccg gggacctcgc
      901 ggcgtgcgac gaggcggagc gcgagatacg ccgccacaaa cgcgaactga tgctgaccat
      961 caccgaggcg cgcgccccgc tcgatcccga cgcgatactg gaactcgccg acatcctctc
     1021 ggagcacagc aagataaatc attcggtcaa acgggtcctg caggcgaacc tcgaccatct
     1081 cgagggacgc ggcgtattca gataccggca cgaaaagatc agttgcccgc ccgccggttg
     1141 agcaccgact gttcctccca acaccccgac agcatcccgc gcgacgacat ccatttgaag
     1201 cggcgcgcaa gttcgtccgg caccgcaccg ggtctttcat gctcccacgg cgtgccgggg
     1261 agcggcatga agatatggcc ccggatgtga gccccctgca aaagaagccg ctccatgagc
     1321 gccaccgtga gcgcgagatc gtcgggcgtc tcgcccggca gtccgaatat caggtccacg
     1381 atcgggtgca gcccggcggc cttcgtattc tcgacggcgc ggtatatgtc cgcgacggtg
     1441 tggccacggt gcatgtgctc aagcatccgg tcgctccccg actgaccgcc gacgacgatg
     1501 atgggattgt cacagtatcg tttgatgacc gccatgcttt cgcgggtcgc gaactcgggc
     1561 cgtatctcgg ccgggaaggt accgaatatc accttgcggc gcggtcccac cgtatcgcgg
     1621 acggcggcga gcagttcgtg gagcggttcg ggggtgcggc catccgggtt gtgccagccg
     1681 agcgcgttgg gcgacaggaa ccggtagtag gcgaactcgc gcgcggcgag ttcccgcacc
     1741 gccgccagta tcgcctcgcg gctccgccac cgttcggcga ccccgtgaaa aaatgaggtc
     1801 tggcagtagc ggcagacgct cgcacagccc cgcgttatct ctatcggccc gtagagtttc
     1861 acgcggtgcg ccatcatcgg gaaccgttcg atatcgaccg gcgccgccgg cgcgccgacc
     1921 agcgcgtcac cttcgagacg acacaggccg cgcaccccct caagcgatct tccctcccgc
     1981 acccgttccg ctatctcacg gatcatctcc tcgccctccc cgaccgccgc aaggtcgaac
     2041 ccccgctcga tgacctcccg tggacgcgca gaggcgtgcg gaccgccgat gatgaagcgg
     2101 gcgttcggga accggccgcg gagatgccgc atctcggcga cgctccgatc gaggtcggcg
     2161 gtcatggcgg aaaacaagaa tactgccgtc gtatcggcgg gcagatcgtc aagttcgcga
     2221 atttcgcgca ccgctttgac cgc
//
LOCUS       DAOQUE010000461         2241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_60415_length_2241_cov_5.028362, whole genome shotgun sequence.
ACCESSION   DAOQUE010000461 DAOQUE010000000
VERSION     DAOQUE010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60415_length_2241_cov_5.028362"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PKH10_11835"
     CDS             complement(<1..344)
                     /locus_tag="PKH10_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28851.1"
                     /translation="MKAKVAFFLYRRDLGAILHALRYAQEARASGGEVRVVLEDKATA
                     LLFELTEPVHAAAALFEELVREKVIVVCRACAAKTETLSLAENRDMVIEDACEGHVPV
                     LAYVESGFTIIT"
     gene            complement(488..>2241)
                     /locus_tag="PKH10_11840"
     CDS             complement(488..>2241)
                     /locus_tag="PKH10_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28852.1"
                     /translation="TITDKNITLWGACVEGTILSPTTPMEDEATDAIIRIDGTNPEHK
                     VVVKNLTLTGGQRRGMNITGITAEVSHVEIVSAKKGGVLVDYGGSLTASSVRIRDTLS
                     DSSNASGVGMALYDDVTVLLDRVILERNRTVGIHAVSTGISQVHFEATDMIVRDTQEQ
                     EGEKDLGNGIFLLNNVTAVLKRVLIERNRNIGLAAATENGDTKCSLQAEDIVVRDTRS
                     TQNDQTEGKGIFVARNTEALLARTLIEENRYVGMVVASDVEGVSARLSASDTIVKDTE
                     AQEAPEAQKSGRGISLEGNVQMTFSRILLQNNKEAGLVAKTENTEFLCSIGGDSLMVR
                     ETKETVDEQKDGRGIHLENNVSANFDRVLVEKNRTMGLSALTLLEGAQGSLQMTDFII
                     RDTQAQKSDHRFGEGVFVQEAFTVDLRRGAIVDNREAGVFLLDALDMYTAPPTVTLSD
                     VKISGTKIRECSELKIDCVYAPDAPFGHGLGVYDGAEATLRNVEITNNTNGIQIRDSK
                     VFSGDECSLAACSGTGIPVCLLLKDNETAINAFELPADYDINTAFAEGKTCYEGNDTN
                     FSSEEQAVPEPPDPPEM"
BASE COUNT          483 a          650 c          548 g          560 t
ORIGIN      
        1 atcgtgatga tcgtgaaccc gctctcgaca tacgccaata ccggcacatg cccttcgcag
       61 gcgtcttcga tcaccatgtc ccggttctcg gcgagagaaa gggtctcggt cttcgcggcg
      121 caggcgcggc agaccacgat gaccttttcg cggaccaatt cctcaaaaag cgcggcggcg
      181 gcatggaccg gctcggtcag ttcgaacagg agcgccgtcg ccttgtcctc aagaacgacc
      241 ctgacctccc cgcccgaggc ccgcgcctcc tgcgcgtacc gcaacgcatg gaggatggct
      301 cccagatccc tccggtacag aaagaacgct accttggctt tcatcggacg acctccttcg
      361 gacagaccgg gtattttcac ctcgtcataa cgggcactaa ggacttgcgt tcattgtgcg
      421 atttgggcgt ggtaatgtca agaaaaagca ccgggggacg aggcaaacta gaacttgaca
      481 aaaaccctta catttccggt ggatctggcg gttccggcac cgcttgttct tcgctggaaa
      541 agtttgtatc gttgccctca taacaggttt ttccttcggc aaaggcggtg ttgatgtcgt
      601 agtcagcagg aagttcaaaa gcgttgatgg cggtctcgtt gtccttgagc aggagacaaa
      661 cggggattcc cgtcccggaa caggcagcaa gggagcactc atcgccggaa aagacctttg
      721 aatctcttat ctgtatgccg ttcgtgttgt tcgttatctc gacattccgc aaggtcgcct
      781 ctgcgccgtc atacacaccg agaccatgcc cgaacggtgc atcgggggca taaacgcaat
      841 ctatcttgag ttcggaacac tcgcgtatct tagtgccaga tatcttaaca tcagacagcg
      901 taactgttgg cggcgcggta tacatatcca aagcatccaa cagaaaaacg cccgcctctc
      961 tgttatcgac gatggctcct ctccgaagat cgaccgtaaa ggcctcttgc acgaaaacgc
     1021 cttccccaaa acgatgatcg cttttctgtg cctgagtatc gcgaatgatg aagtcggtca
     1081 tctgtagcga accctgcgct ccttctaaaa gtgttagcgc cgacaacccc atcgttctgt
     1141 ttttctcaac gagtacgcga tcgaaattcg ccgagacatt gttctccaag tggatgcctc
     1201 tgccatcttt ttgttcgtcc accgtttctt ttgtttcccg taccatcaag gagtcgcctc
     1261 ctatcgaaca aaggaactcc gtattttccg tcttggccac cagccctgct tccttgttgt
     1321 tttgaaggag gatccgggag aaggtcatct ggacatttcc ttccagagat attcctcttc
     1381 cagacttttg ggcttcaggc gcctcctgcg cctccgtatc cttaactatg gtgtcagagg
     1441 cagagagtct cgcggacaca ccttccacat cagaggccac gaccataccc acataccgat
     1501 tttcttcgat cagcgtccgg gctaacaaag cctcggtgtt cctcgcgacg aatatccctt
     1561 tcccttctgt ctggtcattc tgcgtggaac gagtatcccg cacaacgata tcctccgctt
     1621 gaagggagca tttcgtatcg ccgttctccg tcgcggcagc taagccaata ttcctgttgc
     1681 gctctatcag gaccctcttc aaaacagcgg tgacattatt caagaggaat atcccattgc
     1741 ccagatcctt ttcgccttct tgttcttgcg tgtcccgcac gatcatgtct gtcgcctcaa
     1801 aatgcacctg ggagattcct gtcgaaacgg catgaatacc cactgttctg tttctttcca
     1861 gaatgacacg gtccagcaag accgtaacat catcatataa ggccatcccg acaccggaag
     1921 cattgctcga atcggataag gtgtccctta tgcgtacgga cgatgctgtt agcgagccac
     1981 cataatcaac cagaacccct cctttttttg cagaaacgat ctctacatgg gaaacctcgg
     2041 ctgtaatgcc ggtgatattc atacctcgcc tttgtccgcc ggtgagggtt agatttttga
     2101 cgacgacctt gtgctccggg tttgtcccgt caatgcggat gatggcgtcg gtcgcctcgt
     2161 cctccattgg tgtcgtcgga gagagtatgg taccttcgac acaggcgccc cagagggtga
     2221 tgttcttgtc ggtgatggta a
//
LOCUS       DAOQUE010000462         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_60481_length_2240_cov_2.317162, whole genome shotgun sequence.
ACCESSION   DAOQUE010000462 DAOQUE010000000
VERSION     DAOQUE010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60481_length_2240_cov_2.317162"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="PKH10_11845"
     CDS             <1..1673
                     /locus_tag="PKH10_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT28853.1"
                     /translation="ESNDIGVAGVCPWCGIYPVLALEGVEGSAISEEMYLKTYDRFVA
                     DPGIVAVNCSFGMSSEYGEVPMMPGVEESHTRFMRDGRNGKGGAIMYASGNEGVDAHY
                     DGMFQHIFEFERNGKQVSHRLVIVGATSAWDTHIDYSNWGMELGISTPSLSSYPVIGI
                     ATTHIPGTGGLGDYTAYFSGTSAASPFATGAMGFIFSLNPDLTLEEAIDILYRSADRI
                     NPETGFWDENGFSPKFGHGRVNLHKAARLALGHPMCDPLTTGPEELNGLDDDCDSWVD
                     EGLKGEFKVGLPCEKDEDCVMEGYAPEQVRCASRVMGYTMPDNYCVLTVEGEPCPDGS
                     FLGGTGSSLECLRECGPNFDCGRSGFYCTDEIYGVCLPYCSEDTDCPEGNYCDGDICH
                     MTPSPNGGPCASEEDCMYTGSYCDTSKPGGYCFTLCDDYDDSLCPDDSKCIYSARSRT
                     FLCAPSCTSDANCRPDDETHYYRCHVDFQGKEGVCFRPCRNDAECGDDGDCNEDGRCV
                     PWGWVPPDDAPVPDEVIPDDDATDAPVFDFDMAPGGGGSSDGCALVVI"
     gene            complement(1695..>2240)
                     /locus_tag="PKH10_11850"
     CDS             complement(1695..>2240)
                     /locus_tag="PKH10_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28854.1"
                     /translation="TLSKLGDYWILSLQRLNVRTVAVLGRSERKVKGDLNLLVESIAP
                     AVAELFGREVPEPASDNAALPASDPSGTNATPPRELTAMGWNGVGFIAGGGALIIFGG
                     IAQWRTGEAKKDYEAGGTGGDTKTYDAWKAVTITSYVTGGALLAVGTTLLIWDLVADR
                     PVEAALAPLPGGAMVALRFSF"
BASE COUNT          395 a          719 c          712 g          414 t
ORIGIN      
        1 ccgaatcgaa cgatatcggc gttgccggtg tctgcccgtg gtgcggcatc tatccggtgc
       61 tcgcgctcga aggggtggaa ggaagcgcca tcagcgagga gatgtacctc aagacctacg
      121 accgcttcgt cgccgacccc ggcatcgtcg cggtcaactg cagtttcggc atgtcctccg
      181 agtacggcga ggtaccgatg atgccggggg tcgaggagtc gcacacccgc ttcatgcgcg
      241 acggtcgcaa cggcaagggg ggcgccatca tgtacgcctc gggcaacgaa ggggtcgatg
      301 cccactacga cggcatgttc cagcacatct tcgaattcga gcggaacggc aagcaggtgt
      361 cgcaccggct cgtcatcgtc ggtgccacct cggcgtggga cacccacata gactattcga
      421 actggggcat ggaactcggc atcagcaccc cgtcgctttc ctcctacccg gtcatcggga
      481 tcgccacgac ccatatcccc ggcacggggg gacttggcga ctataccgcc tatttcagcg
      541 gcacctcggc cgcctctccc ttcgccaccg gcgccatggg cttcatcttc tcactgaatc
      601 ccgacctgac gctcgaagag gcgatcgata tcctttaccg gagcgcagac cgcataaacc
      661 ccgagaccgg cttctgggac gagaacggat tctcgccgaa gttcggccac ggacgggtga
      721 acctgcataa ggcggcgcgc ctcgcgctcg gccatccgat gtgcgacccg ctgaccaccg
      781 gtcccgaaga actgaacggc cttgatgatg attgtgacag ttgggtcgat gaggggttga
      841 aaggggagtt caaggtcggc ctgccctgcg agaaggacga ggactgcgtg atggagggct
      901 acgcgcctga gcaggtgcgg tgcgcctccc gtgtcatggg ctatacgatg cccgacaatt
      961 attgtgtcct gacggtcgag ggggaaccgt gccccgacgg gtcgttcctc ggcggtaccg
     1021 gcagttcact cgagtgcctg cgggaatgcg ggccgaactt cgactgtggt cggtcgggat
     1081 tctactgcac cgatgagata tacggtgtct gtctgccgta ctgctcggag gacaccgatt
     1141 gccccgaagg taactactgc gacggcgata tctgccacat gacaccctcg ccgaacggcg
     1201 gtccttgcgc gagcgaggag gattgcatgt acaccggctc gtactgcgac acctccaagc
     1261 cgggcggcta ttgcttcacg ctctgtgacg attacgacga ttcgctctgc cccgacgact
     1321 cgaaatgcat ctacagcgcc cggtcccgca ccttcctatg cgccccttca tgcacctcgg
     1381 acgccaactg ccgtcccgac gacgagaccc attactatcg gtgtcatgtc gatttccagg
     1441 ggaaagaggg ggtctgcttc cgtccgtgcc ggaacgatgc ggagtgcgga gatgacggcg
     1501 actgcaatga ggatggtcgc tgtgtgccgt ggggctgggt gccgccagat gacgcgccgg
     1561 tccccgacga ggtcataccc gacgacgatg ccaccgacgc gccggtattc gacttcgaca
     1621 tggcgccggg tggtggcggt tcgagtgatg gctgtgcgct cgtggtgatc tgaggacgcg
     1681 gtatccgcga tctgtcagaa agagaatcgc agggcgacca tcgcgccgcc cggtaacggc
     1741 gccagcgccg cttccaccgg tcggtcggcg acgagatccc atatgagcag ggtcgttccg
     1801 acggccagta gcgcgccgcc ggtcacatac gaggttatcg tcaccgcctt ccacgcgtcg
     1861 taggtcttcg tgtcgccgcc cgtgcctccc gcctcatagt ccttcttcgc ctcgcccgtt
     1921 cgccactgcg cgataccgcc aaagatgatg agtgcaccgc cacccgcgat gaacccgacc
     1981 ccgttccagc ccatggccgt gagttcgcgt ggcggtgtcg cgttcgtgcc ggatggatcg
     2041 ctcgccggca gggcagcgtt gtcggatgcc ggctctggga cctcccggcc gaacagctcg
     2101 gcgaccgccg gcgcgatgct ctcgacgagc aggttgagat cgcccttcac cttccgctcg
     2161 gatcgaccga gcaccgccac ggtgcggaca ttgagccgct ggagggacag tatccagtag
     2221 tcgccaagtt tcgagagggt
//
LOCUS       DAOQUE010000463         2239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_60496_length_2239_cov_3.902930, whole genome shotgun sequence.
ACCESSION   DAOQUE010000463 DAOQUE010000000
VERSION     DAOQUE010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60496_length_2239_cov_3.902930"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /gene="tsf"
                     /locus_tag="PKH10_11855"
     CDS             <1..862
                     /gene="tsf"
                     /locus_tag="PKH10_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNT28855.1"
                     /translation="KNALVEAKGDTEQAVTILREKGLAKAAKKAGRVASEGAVLIRMD
                     GDKKALMLELNCETDFVAKNDGFRELSKTMLDYFDRHGKEEGTLVDMQEKFYDAEIER
                     LTTGAVAKIGENIRPRRFIRYAVKENGFIHSYVHMGGRIGVLVEIAATDAAVLAKPEV
                     REMADDVAMHIAAMNPVCVVPEEFPASLIESEKAIYRVQVLEMGKPENMVDKIVEGKL
                     RKFISESTLVNQTFVKDQELTVGAYVKQVAKKTGAELKVVRFVRFELGEGVEKENKDF
                     ASEVQSQIGK"
     gene            872..1660
                     /locus_tag="PKH10_11860"
     CDS             872..1660
                     /locus_tag="PKH10_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT28856.1"
                     /translation="MRERSLRPLFFFFLLVPALLAAGGPVDRDAFINAFVQKADLVRS
                     LDANIEAAQAEVDRALWQYFPRIKVTFGMAPFPRYEYEPETVGWKRSYHLGDLGVALR
                     AYGEVTLPIYTFGKIEYAREAAEKGVAVKEAEKKIAVLDLRKQAASFYYSHVMASDML
                     NMVDLSIEKIVEAEEKLDQWLYDGKEGVSQNDLIKLRIEKERLLFSKDRVLASQATLK
                     ALFDRVLGPGYALKDEFMLRWISRSAGRRLRAISSSAVPTGASS"
     gene            1657..2193
                     /locus_tag="PKH10_11865"
     CDS             1657..2193
                     /locus_tag="PKH10_11865"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT28857.1"
                     /translation="MNGLGALESLYRFEKSNFFPNLGAMGNYRIDYTSSVDDRGYPLP
                     SSPYNGYGGEAGVGLEFNLNILEQVAKMRKARSEWKAKEYQASFMIEATLLDLRRKYD
                     ELRALDSQVEHLRKAHKMAKGWMTAEFLNYESGMLSTRDLIDAVKAFVEQEYALVNAM
                     YEYNMKVEDLLTTAGFER"
BASE COUNT          491 a          632 c          708 g          408 t
ORIGIN      
        1 caagaacgcg cttgtcgagg cgaagggcga caccgagcag gcggtcacca tcctgcggga
       61 gaagggactt gccaaggcgg ccaagaaggc ggggcgcgtc gcgtcggaag gcgcggtgct
      121 catccgcatg gacggcgaca agaaggcgct catgctggaa ctcaactgcg agaccgattt
      181 cgtcgccaag aacgacggct tccgcgaact ttcgaagacc atgctcgact atttcgaccg
      241 gcacggcaag gaagagggga cgctcgtcga catgcaggag aagttctatg atgccgagat
      301 cgagcgactg acgaccgggg cggtggcgaa gatcggcgag aacatccgtc cgcgccgctt
      361 catccgctat gcggtcaagg agaacgggtt catccactcc tatgtccaca tgggcggtcg
      421 gataggggtg cttgtggaga tcgccgcgac cgacgcggcg gtgctcgcga agcccgaggt
      481 gcgcgagatg gccgacgatg tcgcgatgca catcgccgcg atgaacccgg tctgcgtcgt
      541 cccggaggaa ttccccgcgt cgctcatcga atcggaaaag gcgatatacc gcgtgcaggt
      601 gctcgagatg ggcaaaccgg agaacatggt ggacaagatc gtggaaggca aactccgcaa
      661 gttcatatct gaaagcacgc tcgtgaacca gaccttcgtg aaggatcagg aactcacggt
      721 cggcgcctat gtcaagcagg tcgccaagaa gacgggcgcc gaactgaagg tcgtccgttt
      781 cgtccgcttc gaactgggcg aaggcgtcga aaaggagaat aaggatttcg cttcagaggt
      841 gcagtcccag atagggaagt agcagggggt catgagggaa cggtctctgc gtccgctttt
      901 tttctttttc ctgcttgtcc cggcgcttct tgcggccggt ggaccggtgg acagggacgc
      961 cttcatcaac gcgttcgtcc agaaagccga cctcgtcagg tcccttgacg cgaacataga
     1021 ggcggcgcag gcggaggtgg accgggcgct ctggcagtat ttccctcgta tcaaggtgac
     1081 cttcggcatg gcgccgttcc cgcgctatga gtacgagccc gaaacggtcg ggtggaaacg
     1141 gagttaccat ctcggcgatc tcggcgtcgc cttgcgcgct tatggcgaag tgacgctccc
     1201 gatatacacc ttcggcaaga tagagtacgc ccgggaggcc gccgagaaag gggtcgccgt
     1261 gaaggaggcc gagaaaaaga tagcggtgct cgatctccgc aaacaggccg cttccttcta
     1321 ctactcccat gtgatggcct ccgatatgct gaacatggtg gacctttcca tcgagaagat
     1381 cgtcgaggcc gaggaaaaac tcgatcagtg gctctacgac ggcaaggaag gagtgtccca
     1441 gaacgatctc ataaaactgc gcatagagaa agagcggctc ctgttcagca aggatcgcgt
     1501 gctggcgagt caggcgacgc tgaaggcgct ctttgaccgg gtactcggcc cggggtacgc
     1561 tctcaaggac gagttcatgt taaggtggat ttcccgttca gccgggagac gcttgagagc
     1621 catctcatcg agcgcagttc ctaccggagc ctcatcgtga acggcctcgg cgcgctcgaa
     1681 tcgctctatc gcttcgagaa gagcaacttc ttccccaacc tcggtgccat ggggaactac
     1741 cgcatagatt acacctccag cgtcgacgac cgcggctatc ccctgccgtc gagcccttac
     1801 aacgggtacg gcggcgaggc gggcgtcggg ctggagttca acctgaacat acttgaacag
     1861 gtcgcgaaga tgcgcaaggc gcggtcggaa tggaaggcga aggaatatca ggcctccttc
     1921 atgatagagg cgacactcct cgacctgcgg cgcaaatacg atgaactcag ggcgctcgac
     1981 agtcaggtcg aacacctgcg caaggcccac aagatggcga agggttggat gaccgccgag
     2041 ttcctcaact acgagtcggg catgttgtcc acacgcgatc tcatagacgc cgtgaaggcg
     2101 ttcgtcgagc aggagtatgc tctcgtcaac gccatgtacg aatacaacat gaaggtcgag
     2161 gaccttctca ccaccgcggg gttcgaacga tgaggatgcc gcttttgctc gccctgtccc
     2221 ttttcttctc cacgctggc
//
LOCUS       DAOQUE010000464         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_60586_length_2237_cov_3.384051, whole genome shotgun sequence.
ACCESSION   DAOQUE010000464 DAOQUE010000000
VERSION     DAOQUE010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60586_length_2237_cov_3.384051"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..634
                     /locus_tag="PKH10_11870"
     CDS             152..634
                     /locus_tag="PKH10_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HNT28858.1"
                     /translation="MTALCPCGTGKAYADCCEPLIKGKKKPATATDLLRSRYTAFARA
                     EIKYIIETTHPKAQSAMNEEETRRWATTSQWEKLEILGQQEVDGKTEIEFVATYTQKG
                     NHNRHHEIAIFSKEGGRWYFEDGRAAPQAQVRHEAPKAGRNDPCPCGSGKKFKKCCGS
                     "
     gene            678..1649
                     /locus_tag="PKH10_11875"
     CDS             678..1649
                     /locus_tag="PKH10_11875"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HNT28859.1"
                     /translation="MASFSWERPLVLFSRCLGFDNCRYNAQIIGFEFGRQLLPYLDHA
                     TVCPEMAVGLGVPRDPIRIESDRGKGRRLVQPSTGRDVSADMEKFAEGFLDTLDPEKL
                     DGVILKSRSPSCGIAGVKIYPCTGKCMATGKGPGFFGDAVLRRFPLHAVEDEGRLIDR
                     GLREHFLTRIFLFAEFRRVRAAKKMDALVAFHSRHKYLFKAYNQSKASIIGKIVANHD
                     RLPVAKVLALYERALRETVAKPMRPTAIINAYEHIFGYFSDRLSKQEKEHYFALMDHY
                     RAGRITFNVPAALLKNWALRFNDEYILAQSIFEPYPAELTMIVERSE"
     gene            1990..>2237
                     /locus_tag="PKH10_11880"
     CDS             1990..>2237
                     /locus_tag="PKH10_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28860.1"
                     /translation="MRSVFIILSLLVLSLALFGEEKKPKVAVMEIEDKSGKLSAPLLE
                     NAAEALRTEIGATNKFILISKDRQRKEMIKGQKKESWK"
BASE COUNT          503 a          686 c          650 g          398 t
ORIGIN      
        1 cttcgatgag acccccaaga aggagaaaaa gccggtaggg gaggattttg aggaagagcc
       61 ggccccgaag aagaaagaga aagagaaaaa gaaatacaag aacgagatgg atgaacttga
      121 cgacctgctc ggcgactgag aggatgccca catgaccgcc ctgtgtccct gtggtaccgg
      181 caaggcctac gccgactgct gtgaaccgct catcaagggt aagaaaaagc ccgcgacggc
      241 cacggacctg ctccgaagcc gctacaccgc ctttgcgcgg gccgagataa agtacatcat
      301 cgagacgacc catcccaagg cgcaaagcgc catgaacgag gaggagactc gccgctgggc
      361 caccacctcg cagtgggaga aactcgagat actgggacag caagaggtcg atgggaagac
      421 cgagatagag ttcgtcgcca cctacaccca gaaaggcaac cacaatcgcc accacgagat
      481 agcgatattc tcaaaggagg gcgggcgctg gtacttcgag gacggtcgcg ccgcgccgca
      541 ggcgcaggtc cgtcacgagg cgcccaaggc gggacgcaac gacccctgcc cgtgcgggag
      601 cggcaagaaa ttcaagaagt gttgcggctc ctagccgcgg ttctgatcga tagcgaacgc
      661 actcccggga ggtacgcatg gcctctttct cgtgggaacg acccttggtg ctgttcagcc
      721 gctgtctcgg cttcgacaat tgccgctata acgcccagat catcgggttc gaattcggcc
      781 gacaactgct cccctacctc gaccacgcca ccgtctgccc cgaaatggcc gtcgggctcg
      841 gcgtcccgcg cgacccgatc cgcatcgaga gcgaccgcgg caaagggcga cgcctcgtcc
      901 aaccgtcgac ggggcgcgat gtgtcggccg acatggagaa gttcgccgaa gggttcctcg
      961 acacgctcga ccccgaaaaa ctcgacggcg tcatcctgaa gagccgctcc ccgtcgtgcg
     1021 gtatcgcggg agtgaagata tacccctgca ccggcaaatg catggcgacc ggcaaaggac
     1081 ccggcttttt cggcgacgcg gtgctccgcc ggttcccgct ccacgccgtc gaggatgaag
     1141 gccgcctcat cgaccgcggc ctgcgggaac atttcctgac gcggatattc cttttcgccg
     1201 aattccggcg cgtccgcgcg gcgaaaaaga tggatgcgct cgtcgcgttc cacagccgcc
     1261 acaagtatct tttcaaggcc tacaatcagt cgaaggcgag catcatcggc aagatcgtcg
     1321 ccaaccacga ccgtctgccg gtcgccaagg tgctggcgct ctacgaacgg gcgttgcgcg
     1381 aaacggtggc aaagccgatg cgccccaccg ccatcatcaa cgcctacgaa catatcttcg
     1441 gctacttctc cgaccgcctc tctaaacagg agaaggaaca ctacttcgcg ctgatggacc
     1501 actaccgcgc cggacggatc accttcaatg tgccagcggc gctgctgaag aactgggcgc
     1561 tccgtttcaa cgatgagtat atcctcgccc agagcatctt cgagccctac ccggccgaac
     1621 tgactatgat cgtcgaacgg agcgaatagc ggcttcctcg ccctgtgaag ggagaggatt
     1681 gaggtgaggg tgttctgaaa accctcttcc ggcctgacgg ccaccttctc cctcgatagg
     1741 tagaaggttt tgcgtcgacg tttttcttca acctcatcct aaccttctcc ggtgcggaga
     1801 agggaccgat ttctccctcg ccaccagcgg gaaagccaac aggcgcccct gttctccctc
     1861 tccactttgg agagggccgg ggtgaggttt gagagggctg gggtgaggtt ttgtggtggc
     1921 cttttccttg aattcccctc ccctttttat tacaatcagg cgttctcatg attgaaatcc
     1981 gggggtctca tgcgtagcgt atttatcatc ctttcgcttc tggtgctcag tctcgcgctt
     2041 ttcggcgagg agaagaaacc caaagtcgcc gtcatggaga tcgaagacaa gtccggcaaa
     2101 ctctccgcgc cgctgcttga gaacgccgcc gaagcgctcc gcaccgagat cggcgcgacc
     2161 aacaaattca tccttatctc aaaggaccgg cagcggaaag agatgatcaa gggacagaag
     2221 aaggagagtt ggaagga
//
LOCUS       DAOQUE010000465         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_60694_length_2234_cov_3.783387, whole genome shotgun sequence.
ACCESSION   DAOQUE010000465 DAOQUE010000000
VERSION     DAOQUE010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60694_length_2234_cov_3.783387"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKH10_11885"
     CDS             complement(<1..463)
                     /locus_tag="PKH10_11885"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNT28861.1"
                     /translation="MKGAIDEDVVAVALSIRESIRRSGAGTRLVVNDRPDIAARCGAP
                     VVHVGDEDMSPSQVRRRYPDLAIGWSAHTLDDIERANSFDLLYVAFGPVFPSATKVTG
                     RAPVLALAPEALRLSRHPLVFIGGIAPANIEQLPFRHDTQAAVIGALQDFVE"
     gene            complement(556..1296)
                     /locus_tag="PKH10_11890"
     CDS             complement(556..1296)
                     /locus_tag="PKH10_11890"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT28862.1"
                     /translation="MIAGISHATEAMRRFVTKVQPGKSILIGGEPGVGKYHLARKIVN
                     TYYKNDGIRSRYARIVKAEFGLPDPLTDEVVLVEDIAQFEELSTRIPQALWIPPLRER
                     LEDIPPLLECFVTRSDDYDFWCLPENLSHLLAYWWPYNVAELRRVVTTREGRDNLPHA
                     RTREILSHLPANQLISMKMESFWENIGKNARPGKFFQLFLDSVEREFIKTALSRCEWS
                     VGETARLLDMHRNTLNQKMRKFRIVKKK"
     gene            complement(1293..2222)
                     /locus_tag="PKH10_11895"
     CDS             complement(1293..2222)
                     /locus_tag="PKH10_11895"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNT28863.1"
                     /translation="MRLYLAPMAEISTAAFREICYRNGADVCYSEMISAKAVMFNNKK
                     TIKMAMIRPEEPRTFVQIFGSDPDDIAGAIRRIEELCPPYGFDINMGCPMKKVIKGRN
                     GAFLMTDPDKVRRVVRAARAATKKPLSVKIRKGYIHENGSEVAAIIEEEGADLLVIHP
                     RLKTQMFLPGTCDPDFSIRIAVERKIPVIHSGDITGPKDIERFADSRVAGVMIGRGTF
                     GRPWIFDEIRHGVAKDAEERKAIMKLHFNAIRIYSRQLSEKAMMSEIKKNAVWYSRGY
                     EGAAEFRNRVYQTAWAESDLMRFVGDFFGMGLV"
BASE COUNT          437 a          683 c          661 g          453 t
ORIGIN      
        1 actcaacaaa atcctgaagc gcaccgatga ccgccgcctg cgtgtcgtgg cggaagggca
       61 actgttctat gttggccgga gcgatacccc ctatgaagac caacggatga cgcgaaagac
      121 ggagcgcctc cggcgccagt gcgaggaccg gcgcccggcc ggtgaccttg gtggcgctgg
      181 ggaacacggg accgaaggcg acatagagga gatcgaagga gttggcgcgt tcgatgtcgt
      241 cgagcgtatg ggccgaccag ccgatcgcga gatccgggta tcggcgccgg acctgcgacg
      301 gcgacatatc ctcgtcaccg acatgcacca ccggcgcacc gcaacgcgcg gcgatatcgg
      361 ggcgatcgtt caccaccagc cgggtgccgg caccggacct gcggatgctc tcccgtatcg
      421 aaagcgccac ggccacgaca tcctcgtcga tcgcgccctt catgcgcagt tgcagtaagg
      481 acggtctcag aacattcagg atatcttccg gatcttccgg cacggcgccg atgatggggt
      541 ataccgaccg gcgtgtcact ttttcttaac gatgcggaat ttgcgcatct tctgattcag
      601 ggtattgcga tgcatatcga gaagacgcgc cgtctcaccg accgaccatt cgcagcgaga
      661 cagagcggtc ttgatgaatt cccgctccac cgaatcgagg aaaagttgga agaacttgcc
      721 cgggcgggcg ttcttgccga tgttctccca gaaactctcc atcttcatcg aaatgagttg
      781 attggccggc aggtgggaga ggatctctcg cgtccgcgcg tgcgggaggt tgtcgcgccc
      841 ttccctggtc gtgaccacgc gccgcagttc cgcgacattg taaggccacc agtaggcgag
      901 caggtgcgag aggttctcgg gaagacacca gaaatcgtag tcatcgctcc gggtaacgaa
      961 acattcgagg agcggcggga tgtcctccag ccgttctctg agcggcggta tccagagcgc
     1021 ctgcgggatg cgggtcgaaa gttcctcgaa ctgcgcgata tcctcgacga gcaccacctc
     1081 gtcggtcagg ggatcgggca gaccgaactc ggccttcacg atgcgggcgt accgcgaacg
     1141 gatgccgtcg ttcttgtagt aggtgttcac gatcttgcgg gcgagatggt atttgccgac
     1201 acccggttca ccgccgatga gtatcgactt gccgggctgg accttggtga cgaagcgacg
     1261 catcgcctcg gtggcgtgac tgatgccggc tatcatacga gacccatccc gaagaaatct
     1321 ccgacgaatc gcatcagatc cgattccgcc cacgcggtct ggtagacacg gttgcggaac
     1381 tcggcggcac cttcgtaccc gcgcgaatac cacaccgcgt ttttcttgat ctcgctcatc
     1441 atcgccttct cggataactg ccgcgagtat atccgaatgg cgttgaaatg caatttcatg
     1501 atggcttttc tttcctccgc atccttcgcg acgccatgcc gtatctcatc gaatatccat
     1561 ggacgaccga aggtcccgcg gcctatcatg acgccggcca cccgcgaatc ggcgaaccgt
     1621 tcgatatcct tcgggccggt gatatcgccc gaatggatga ccggtatctt ccgctcgacc
     1681 gcgatccgga tcgaaaaatc ggggtcacag gtgccgggga ggaacatctg cgtcttgaga
     1741 cgcggatgga ttacgagcag gtcggcgccc tcctcctcga tgatggccgc cacctccgac
     1801 ccgttctcgt ggatgtagcc cttgcgtatc ttcaccgaaa gcggtttttt cgtcgccgcc
     1861 cgcgcggccc ggacgacacg ccgcaccttg tcgggatcgg tcatcaggaa ggcgccgtta
     1921 cgccccttga tgaccttctt catcggacag cccatgttga tatcgaatcc gtacggcggg
     1981 cacaattcct ctatgcgccg tatcgcgccg gcgatatcgt cgggatccga cccgaagatc
     2041 tggacgaagg tccgcggctc ctcgggacgg atcattgcca tcttgatggt cttcttattg
     2101 ttgaacatca ccgcctttgc cgatatcatc tcgctgtagc agacatcggc accgttccgg
     2161 tagcatatct cacgaaaggc ggcggtgctt atctccgcca tgggagcaag gtagagtcgc
     2221 atacctcacc atgt
//
LOCUS       DAOQUE010000466         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_60792_length_2232_cov_4.114837, whole genome shotgun sequence.
ACCESSION   DAOQUE010000466 DAOQUE010000000
VERSION     DAOQUE010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60792_length_2232_cov_4.114837"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..1645)
                     /gene="gdhA"
                     /locus_tag="PKH10_11900"
     CDS             complement(302..1645)
                     /gene="gdhA"
                     /locus_tag="PKH10_11900"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNT28864.1"
                     /translation="MNSYVSAVLNDLKTRYHWEKEFLQAAEEVLDSLTPIVEREPKYK
                     AHKILERIVEPERTIMFRVPWIDDKGEFQVNRAYRVQFNSAIGPYKGGIRFHPNVTLS
                     TLKFLGFEQIFKNSLTGLPMGGGKGGSDFNPNGKSDGEVMRFCQSFMTELFRHIGPDT
                     DVPAGDIGVGGREVGYMFGQYKRLANEFTGVLTGKGIKWGGSLIRPEATGFGVVYFAE
                     EMLKSKGDSMKGKKVAVSGFGNVSWGAVIKANDLGAKVVTISGPDGYVYDKDGVSGDK
                     IEYMLEMRASGRDRVEDYAKKFKGVEFHAGKRPWEVAVDVALPCAIQNELDDKDAATL
                     VKNGCKVVVEGSNMSSTLDAVKLFVEKKILYAPGKASNAGGVATSGLEMSQNSMRLGW
                     PREEVDARLRQIMVGIHHSCVTAAKEYGFEGNYVVGANIAGFKKVADAMVEQGLV"
     gene            1912..>2232
                     /locus_tag="PKH10_11905"
     CDS             1912..>2232
                     /locus_tag="PKH10_11905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT28865.1"
                     /translation="MSDEALLRTALTRNERAWRELVRRYHGKVSATVRRIYGPRSETE
                     DIVQEIFIELAKSAKNFRHDSSLSTFLYRIATNTTYRYIKRHKEAHMVSEPAEFFANL
                     PSENE"
BASE COUNT          487 a          649 c          624 g          472 t
ORIGIN      
        1 cttctcatgt ttttcgacat agcagtagga acatttcagt gtacactcac gggtcaggaa
       61 caggatcgcg tgcatggctc actccagcaa agacgcccct ttagtgtaag aaaaaggaac
      121 cttttatcga agaaagtgcg ccaccccctt ttcgcaggac gcctcatttt atcaggatga
      181 cagttagttg caaggaaaag gtgaaaccga attgtagggg cgttcccatc cgggacgccc
      241 tgaataaaaa ggatgtcgga agaagagggc gtgccgttgg cgcgccccta catttggatt
      301 ttcacacgag accctgttcg accatcgcgt cggcgacctt cttgaagccg gcgatgttcg
      361 caccgaccac atagttgccc tcgaagccgt attccttcgc cgcggtgacg caggagtgat
      421 gaatgccgac catgatctgg cggaggcgag catcgacctc ttcgcgcggc cagcccagac
      481 gcatcgagtt ctggctcatc tcgaggccgc tggtcgcgac gccgccggcg ttggatgcct
      541 tgcccggcgc gtagagtatc ttcttctcga cgaagagttt caccgcatcg agcgtggagg
      601 acatgttcga cccctcgacc acgaccttgc agccgttctt cacgagcgtc gcggcgtcct
      661 tgtcatccag ttcgttctgg atggcgcagg gaagcgccac atcgaccgcc acttcccacg
      721 gccgtttgcc ggcgtggaac tcgactccct tgaacttctt cgcgtagtcc tcgaccctgt
      781 cgcggccgct ggcgcgcatc tcgagcatgt actcgatctt gtcgccgctg accccgtcct
      841 tatcgtacac atagccgtcg ggaccggaaa tggtgacgac cttcgcgccg aggtcgttgg
      901 ccttgatgac cgcgccccac gacacattgc cgaaaccgga gaccgcgacc ttcttgccct
      961 tcatcgagtc gccctttgac ttgagcatct cttcagcgaa gtagaccacg ccgaagccgg
     1021 tcgcctcggg acggatcaga gagccgcccc acttgatccc cttgccggtc aggacgccgg
     1081 tgaactcgtt ggcaagccgc ttgtactggc cgaacatgta gccgacctca cggccgccca
     1141 cgccgatgtc acccgccggg acatcggtgt cgggaccgat gtggcggaag agttcggtca
     1201 tgaacgactg gcagaatcgc atcacttcgc cgtcggactt gccgttcgga ttgaaatcgg
     1261 aaccgccctt accgccgccc atgggaagac cggtcagcga gttcttgaat atctgttcga
     1321 agccgaggaa cttgagtgtt gagagcgtca cattgggatg gaagcggatg ccgcccttgt
     1381 aggggccgat ggcgctgttg aattgcacgc gataggcgcg gttgacctgg aactcgccct
     1441 tgtcgtcgat ccacgggacg cggaacatga tggtgcgttc cggctccacg atgcgctcaa
     1501 ggatcttgtg ggccttgtac ttgggttcgc gctcgacgat cggggtaagc gaatcgagca
     1561 cctcttcagc ggcctgcagg aactccttct cccagtgata ccgggtcttg aggtcgttga
     1621 ggaccgcgct cacataactg ttcatcgtat cctccgatga ggctgaatta ataacgttcc
     1681 gcgcacgatg gcggaagccg atatcattat agacaccgtt tcagagccat tgtcaagcaa
     1741 aatcgttagg aattcattag ttgaagaatt tacctataga atacagggaa ttacaaaata
     1801 ctaaaacttt ttttgcttgt ttcagaaaat atctcaataa atagaggggc ggagaaaaac
     1861 taaaggagca tgaaaacggt ggcggacaca aaccccgtgg ttcttggaga aatgagcgac
     1921 gaggccctgc tcagaactgc gctgacccgc aacgaacggg catggcggga actggtgcgg
     1981 cgctatcacg gtaaggtgag cgcgacggtg cgccggatct acgggccgag atcggaaacg
     2041 gaggatatcg tgcaggagat cttcatcgaa ctcgcaaaat cggcaaagaa cttccgccat
     2101 gactcttccc tgtcgacctt cctctaccgt atcgcgacca acaccaccta tcgctatatc
     2161 aagcggcaca aggaggccca tatggtctcc gaaccggccg agttcttcgc caacctccct
     2221 tccgagaacg ag
//
LOCUS       DAOQUE010000467         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_61432_length_2217_cov_5.032840, whole genome shotgun sequence.
ACCESSION   DAOQUE010000467 DAOQUE010000000
VERSION     DAOQUE010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61432_length_2217_cov_5.032840"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..439
                     /locus_tag="PKH10_11910"
     CDS             179..439
                     /locus_tag="PKH10_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28866.1"
                     /translation="MPLRRLILPLLVAVLVAAGLALLAFLGTSERTDFMAHLFGFVAG
                     LGVGLVALPALGRGVLMGPPGRMLLSLCSLLLVLAAWARALV"
     gene            541..>2217
                     /locus_tag="PKH10_11915"
     CDS             541..>2217
                     /locus_tag="PKH10_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004516573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28867.1"
                     /translation="MCRNLSCVLTAGVFALSLLLFAAGCTPTEEGEDLLADGMATADG
                     ETADGPGPDDATGDLLVTDTDIPLSDCGCDDPNGDEDGDGIPNGVEGCGDRDIDGAPN
                     CLDAEADGDGISDSAEAGPDPTNPVNSDGDEQPDYLDKDSDDDGLADSEEATYGTDPT
                     KADTDDDGTDDLAEIVYAQENGYDPGQQAIDPEKKVPDGMFYVVLPYNAKNPAQRELD
                     FSTNIDAADVLIFLDNSGSMMDETENLKQGIQTDIIDPITQAIDNVSFGLVTYAWDPF
                     YKLYQAMIMDAETVKNSVANVGECSGTELPTAAMVYAATGAPIQFTIQGCNPMNGQCG
                     PGAWGAIPNPDVIVDFPKMDCTGELGTLGGVCFREKAMHIFIQISDEAYTQCPDPANY
                     TNYNECKTIGGHFPDIIEAIAALNSIGAKFIGIDTGFDDENGGETSFCKQDYTMMAEA
                     TGSLDGSGKNFNSHTGSPDGSGLSGMIADAVVSLMTYIDMNVTTGSLSDEQCDGHSAA
                     EFVRSSTPVKADPIDGISGQDETTFFSVKQGTTVKKVVSSCPEIPSIGSAL"
BASE COUNT          464 a          666 c          677 g          410 t
ORIGIN      
        1 gcaaggcgtc gggcctgatg acccagaaga tccccgagca gatcgagatc gtcgacctcc
       61 tgccccgcaa cccggcgggc aagatcctga agaacgacct gaagaaggcg tacgcagggg
      121 aaggctgaca ggggatggct gaccccgtcc cggtggcgtg cgcccgtagc ctgccggggt
      181 gccgctgcgc cgcctgatcc tgccgttgct cgtggcggtg ctcgtcgccg ccggacttgc
      241 gctcctcgcc tttctcggca cgagcgaacg gaccgatttc atggcgcacc tattcggctt
      301 tgtcgccggt ctcggcgtcg gtctcgtggc gcttcccgca ctgggtcgcg gggtgctcat
      361 ggggccgccc ggtcgaatgc ttctgtcgct gtgctctctt ctgctcgtcc ttgcggcttg
      421 ggcccgcgca ctcgtctgat ccgccaagag gttattgaca aaatccttcc catcgggtag
      481 cctcgttccg cgagttgaaa aaaaacagat cggtatggtc accagaggat ggaggggatg
      541 atgtgtcgta acctttcgtg tgtgttgaca gcgggcgttt tcgctttatc tctcctgctt
      601 tttgcggcgg gttgtacgcc gaccgaggag ggagaggatc tgctcgccga cgggatggca
      661 acggccgacg gggagacagc agatgggccc ggacccgacg atgcgacggg ggatctgctc
      721 gtgaccgata ccgatatccc actctccgac tgcgggtgcg atgatccgaa cggcgacgaa
      781 gatggcgatg gcatccccaa cggtgtcgag ggctgcggcg accgagacat cgacggcgcg
      841 cccaactgcc ttgacgctga agcggacggt gacggcattt ccgatagcgc cgaggcgggc
      901 cccgatccga ccaacccggt gaacagcgac ggtgacgaac aacccgacta tcttgacaag
      961 gattccgacg acgacggtct ggccgactcc gaagaggcga cctacgggac cgatccgacg
     1021 aaggccgata ccgatgacga tggcaccgat gacctcgccg agatcgtcta tgcccaagag
     1081 aacgggtatg atcccggtca gcaggcgatc gatcccgaaa agaaggtccc tgacggcatg
     1141 ttctatgtcg ttctgcccta caacgccaag aatccggcgc aacgggaact tgatttcagt
     1201 accaacatag acgcggccga tgtcctcatc tttctcgaca actccggctc gatgatggac
     1261 gagaccgaga atctcaagca ggggatacag accgacatca tcgatccgat aacgcaggcg
     1321 atcgataatg tctcgttcgg attggtcacc tatgcgtggg atcccttcta caaactgtac
     1381 caggccatga tcatggacgc cgaaacggtc aagaattcgg tcgccaatgt gggggaatgt
     1441 agcgggaccg aactgccgac ggccgccatg gtctatgccg ccaccggtgc ccccatacaa
     1501 ttcacgatac agggctgcaa tccgatgaat ggtcagtgtg gccccggcgc gtggggtgcg
     1561 atccccaacc ctgatgtgat cgtcgacttc ccgaagatgg actgcaccgg cgagttgggt
     1621 acgctcggcg gcgtatgttt ccgcgaaaag gcgatgcaca tcttcatcca gataagcgac
     1681 gaagcctaca cccagtgtcc cgacccggcc aactatacga actacaacga atgcaagacc
     1741 ataggaggcc acttccctga catcatcgag gcgatcgcgg cactcaacag catcggagcg
     1801 aaattcatag gcatcgacac cgggttcgac gacgagaacg gcggcgagac ctctttctgc
     1861 aagcaagact atacgatgat ggccgaggcg accggatcgc ttgatgggag cggtaagaac
     1921 tttaactcgc acaccggcag tccggacggt agcggcctct ccggcatgat agccgatgcg
     1981 gtcgtgtcgc tcatgaccta catcgacatg aatgtgacga ccggcagtct atcggacgag
     2041 cagtgcgacg gccattcggc cgccgagttc gtgagatcct caacccccgt caaggccgat
     2101 cctatcgacg ggatctcggg gcaggatgaa accaccttct tctcggtcaa gcaggggacc
     2161 accgtgaaga aggtggtttc atcctgcccc gagatcccgt cgataggatc ggccttg
//
LOCUS       DAOQUE010000468         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_61433_length_2217_cov_4.744218, whole genome shotgun sequence.
ACCESSION   DAOQUE010000468 DAOQUE010000000
VERSION     DAOQUE010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61433_length_2217_cov_4.744218"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2195)
                     /locus_tag="PKH10_11920"
     CDS             complement(<1..2195)
                     /locus_tag="PKH10_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNT28868.1"
                     /translation="MALTKLELVVSDNLVTFTPDASLSYGTVVRVVWPGSDDPTANVV
                     KSDRATARGGQLPEDASYIFESEITPDLIVTGTFPGEAEKGKPIDGSITVNFNSAVDC
                     ATLVIGTTITLAFDSTDPVAPDTAIPATISPACADGDTAVTIVPDAPIKYSRDVVVTL
                     TDAVATVEAAQANEHADPLMGHLRGGYTFHYSTMDPPPLGVVTIGTGSGHFTIPRTDT
                     IIVGFSEGVLQASAVLAATLDDPDATVIVEDVTGLADPLNDTAHSVIPGTLTYNNTDD
                     EPDNAELIGDDFFMTFTPEAPYQYGTLIRITIPGSDDPTASVVRSDRATTRGGQHTLS
                     TVFLVEVERLEELRVIATSPAHDNRDVPHTATQITVTFNYAPDCTTINGANIFVTYDD
                     GTFVTDPVAPGGTVTGSWACTDGEATVTFTAEEEFGYGRDLLVHLTSDVRDERAAGDA
                     VNPNDPTQGYLVPPFSFGFGTEHLDKLYVISSNAAGSSSFDPDLPIIITFNQPAACAT
                     LTDATFFINKGTTSDPAQKLTATITCASPSSATVQLEPDDSDCGPEALCYDSDYTVTL
                     EGGYTGVCSPDKTPGDQSDDGCIASPEYTFSFHTAKQGEFVAVIYPPNGATGVSPSVN
                     PTVTFSAAVNTASVETDLGVDADGIIPNICLLPGVQYDCSDPSAIAMTIEWTGGDTLA
                     TLKPTATLTPDSDYTIVVSKDIEDIDGRTLTGFYTSTFHTGTAGLLIYVV"
BASE COUNT          369 a          596 c          829 g          423 t
ORIGIN      
        1 accacataga tcaacagacc tgcggtgccg gtgtggaagg tggaggtgta gaagccggtc
       61 agcgtccggc cgtcaatgtc ctcgatgtcc ttcgagacga cgatggtgta atcgctgtcc
      121 ggagtaaggg tggccgtcgg tttcagggtg gcaagggtgt cgccgcccgt ccattcgatg
      181 gtcatcgcga tcgccgaagg atcggagcaa tcgtactgga cgcccgggag caggcagatg
      241 ttcgggatga tgccgtcggc gtcgacgccg aggtcggtct cgacgctcgc ggtgttgacc
      301 gcggcgctga aggtaacggt cggattaaca ctcggagaga caccggtcgc gccgttgggc
      361 ggatagatga ccgcgacgaa ctcgccctgc ttggcggtgt ggaacgagaa ggtgtactcg
      421 ggcgaggcga tacagccgtc atcgctctgg tcacccgggg tcttatcggg cgaacagacg
      481 ccggtgtagc cgccttccag agtaacggtg taatcgctgt cgtagcagag cgcctcgggg
      541 ccgcagtcgc tgtcatccgg ctcgagctga acggtcgcgg aactcgggct ggcgcaggtg
      601 atcgtcgccg tcaacttctg cgccggatcg ctcgtggtgc ccttgttgat gaagaaggtc
      661 gcgtcggtca gggtcgcgca ggcggccggc tggttgaagg tgatgatgat cggcaggtcg
      721 gggtcgaacg acgacgatcc cgcggcgttg gaggagatga catagagttt gtcgagatgt
      781 tcggtgccga agccgaacga gaagggcggg acgagatagc cctgcgtcgg atcgttcggg
      841 ttgacggcgt cgcccgccgc ccgttcatcg cggacatcgc tcgtcagatg gacgagcagg
      901 tcgcggccgt agccgaactc ttcctcggcg gtgaaggtga cggtggcctc gccgtcggtg
      961 caggcccacg aaccggtgac cgtgccgccg ggagcgaccg gatcggtgac gaaggtgccg
     1021 tcgtcatagg tgacgaagat gttcgcgccg tttatcgtcg tgcagtcggg ggcgtagttg
     1081 aaggtgaccg ttatctgcgt cgcggtgtgc ggcacatcgc ggttgtcgtg ggccggagag
     1141 gtagcgatga cgcgcagttc ctcgagccgt tcgacctcta cgaggaaaac ggtgctgagc
     1201 gtgtgctggc cgccgcgcgt ggtggcgcgg tccgatctca cgacagaggc ggtcgggtcg
     1261 tcggagcccg gtatcgtgat gcggatgagc gtgccgtact ggtagggggc ctcgggggtg
     1321 aaggtcatga agaagtcgtc gccgatcagt tcggcgttgt ccggctcatc gtcggtgttg
     1381 ttgtaggtaa gagtgcccgg tatcacgctg tgcgctgtgt cattcagcgg gtcggcgagg
     1441 ccggtgacat cctcgacaat gacggtcgcg tcggggtcgt cgagggttgc ggcaaggacc
     1501 gcggatgcct gaagaacgcc ttcggagaag ccgacgatga tggtgtcggt gcgcggtatc
     1561 gtgaagtggc cgctaccggt gccgatggtg acgacgccga gcggcggcgg gtccatcgtg
     1621 ctgtagtgga acgtgtagcc gccgcgcagg tgccccatga gcgggtcggc atgttcgttc
     1681 gcttgggccg cctcgacggt cgctaccgcg tcggtgagcg tcaccacgac atcgcggctg
     1741 tactttatcg gcgcgtcggg cacgatggtg accgccgtgt cgccatcggc gcaggcgggg
     1801 cttatcgttg ccggtatggc ggtgtcgggc gctaccggat cggtcgaatc gaaggcgagc
     1861 gtgatcgtcg tgccgatcac gagcgtggcg caatcgaccg cgctattgaa attgaccgtg
     1921 atcgagccgt ctatcggttt gcccttctca gcctcgccgg ggaaggtgcc ggtgacgatg
     1981 aggtcgggcg ttatctcgct ttcgaagata taggaggcat cttccggcaa ctggccgccg
     2041 cgggcggtcg cacggtccga cttgacgaca tttgcggtcg gatcgtcgga gccgggccat
     2101 acgacgcgga cgacggtgcc gtaggaaagc gacgcgtcgg gggtgaaggt aacgaggttg
     2161 tccgagacaa ccagttccag cttcgtcaga gccacagccg cgtggaagga taagaaa
//
LOCUS       DAOQUE010000469         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_61525_length_2215_cov_4.603241, whole genome shotgun sequence.
ACCESSION   DAOQUE010000469 DAOQUE010000000
VERSION     DAOQUE010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61525_length_2215_cov_4.603241"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PKH10_11925"
     CDS             complement(<1..1140)
                     /locus_tag="PKH10_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT28869.1"
                     /translation="MSEIPRNIEANIEKWLFKKKVITIFGARQVGKTTLAKRLLEKHG
                     DPKHYYDGDILSVQEVFRSQDPEVCRRMFGSARLIVLDEAQQVPDIGMALKIIHDTLP
                     EVQVIATGSSSFELGNRTGEPLTGRGLSFILYPFSLRELEHRHTPIEVHAALPFHLRF
                     GLYPEIVDKNKDDARVLLDDLVSKYLYKDVLAFEQLKKPDLLLRLLKLVALQLGSEVS
                     LNELATTLGVGRATVERYLDLLEKCFVIFRLKSFSRNLRTELTRKEKIYFYDVGVRNG
                     LISRFQPLDVRDDVGPLWENFCIVERMKALAAHGMRRNFYFWRTHGQKELDLIEEYDG
                     RLDAYEFKWKEGKIRTAVKREFTDAYPGSTITVVSSKSYHPFFAGE"
     gene            complement(1230..2063)
                     /locus_tag="PKH10_11930"
     CDS             complement(1230..2063)
                     /locus_tag="PKH10_11930"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT28870.1"
                     /translation="MKKNVSCFALIIGVFAFFGCDNGLGNNSPSYIGAMIDIPAGEIQ
                     MGCNETVDDQCSEDEAPYHAVTLSAYKIGKYEVIVGEYQQCVTNGACNNSGEYDHYYT
                     NNDSSFCNLGATGKESHPMQCVTWYGAKAYCEWIGGRLPTEAEWEKAARGTDGRKYPW
                     GNEDATCDYAVMFDPNAGGGGCGTGGTMPVGSKPNGVSPYGAYDMAGNVREWVNDWYA
                     SDYYAASPTNDPTGPETGDYRVSRGGSWLSVDGRGLRASYRSHGHPDGNDGGGGFRCA
                     K"
BASE COUNT          457 a          655 c          596 g          507 t
ORIGIN      
        1 ctctccggca aaaaaaggat gataggattt cgatgaaacg accgtgatcg tgctccccgg
       61 gtaggcgtcg gtgaattcgc gctttactgc ggtgcggatc ttaccctctt tccacttgaa
      121 ctcgtaggca tcgagccgcc cgtcgtattc ctcgatgaga tccaattctt tctgtccatg
      181 cgtacgccag aaataaaagt tgcgccgcat cccgtgcgcc gcgagcgctt tcatccgctc
      241 gacgatgcag aagttctccc acaacgggcc gacatcatcg cgcacatcga gcggctgaaa
      301 acgggaaatg agaccgttcc tcaccccgac atcgtagaag tatattttct ccttgcgcgt
      361 cagttcggta cgcaggttcc gcgaaaaaga cttgagccgg aaaatgacga aacacttctc
      421 caataggtcg agatatcgct ctaccgtggc gcggccgacc ccgagcgttg tggccagttc
      481 gttgagggag acctcgcttc ccagttggag cgcgacgagt ttgagaagac ggagtagaag
      541 atcgggcttt ttcaactgct cgaaggcgag cacatccttg tagagatatt tggagaccag
      601 atcatcgagc agaacgcgcg catcatcctt gtttttgtcc acgatctcgg gatacaaacc
      661 gaaccggaga tggaacggga gggcggcgtg aacctcgatc ggcgtgtgac gatgctcaag
      721 ttcccgcagg gaaaagggat agaggatgaa ggaaaggccc cgtcccgtaa gcggctcgcc
      781 ggtacgattc ccgagttcga aactgctgga gccggtcgcg atgacctgaa cttcgggaag
      841 cgtgtcgtga atgatcttga gcgccatccc gatatcgggg acctgttgcg cttcatcgag
      901 gacgatcagg cgcgccgagc cgaacatccg gcggcacact tccgggtcct gcgaccggaa
      961 tacctcctgc accgagagga tatcgccatc gtagtagtgt ttcgggtcac cgtgcttttc
     1021 cagcaggcgc ttcgccagcg tggtcttgcc cacctgccgc gccccgaaga tggtgatgac
     1081 cttcttttta aaaagccatt tctcgatgtt cgcttctatg tttcgcggga tttcgctcat
     1141 ccattcacct caaaaaagga caaacaacta tcatttttta tataaatttt gatagttgtc
     1201 aagcgttttt tacgaattcg tcagcgagat cacttcgcgc accggaaccc gccgccgccg
     1261 tcgttgccgt ccgggtggcc gtggctgcgg taggacgcgc gcaggccgcg gccgtcgacg
     1321 ctgagccacg acccgccgcg cgacacgcgg taatcaccgg tttctggccc cgtggggtcg
     1381 tttgtaggcg atgcggcata gtaatccgag gcgtaccagt cgttcaccca ctcccgcaca
     1441 ttgcccgcca tgtcataggc gccgtagggc gacaccccgt tcggcttgct tcccaccggc
     1501 attgtcccac cggtgccaca tccaccgcct ccagcgttcg ggtcgaacat gacggcataa
     1561 tcgcaggtcg catcttcatt gccccacggg tattttctcc cgtcggttcc tctcgccgcc
     1621 ttctcccatt ccgcttcggt cggcaatctg ccgcctatcc actcacagta cgcctttgct
     1681 ccgtaccagg tcacgcactg catcgggtgg ctctccttgc ccgttgcgcc gaggttgcag
     1741 aacgaagagt cgttgttggt gtaatagtga tcatactcgc cgctgttgtt gcaggcgcca
     1801 ttggtgacgc actgctgata ttcgccgacg atcacttcgt acttgcctat cttgtaggcc
     1861 gacagagtta ccgcgtgata gggtgcttca tcctcggagc attggtcgtc caccgtttcg
     1921 ttacatccca tctggatttc accggcgggg atgtcgatca tggcaccaat ataactcggc
     1981 gaattgttgc ctaatccatt atcacaacca aagaaagcaa acacccctat aataagcgca
     2041 aaacaggaca cattcttttt catcgccgcc tcctatatcg tccgggtgga ggaactgatc
     2101 ttgaagccct tcgtgaggat gtgcggcacg agacccgccg agatatcgaa ctcgaagtag
     2161 ttgtcggaat tggggaccac ctgttgttcg ctaccgaccg ccgcgagatc ctcga
//
LOCUS       DAOQUE010000470         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_61527_length_2215_cov_4.262037, whole genome shotgun sequence.
ACCESSION   DAOQUE010000470 DAOQUE010000000
VERSION     DAOQUE010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61527_length_2215_cov_4.262037"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /gene="dnaX"
                     /locus_tag="PKH10_11935"
     CDS             <1..1383
                     /gene="dnaX"
                     /locus_tag="PKH10_11935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNT28871.1"
                     /translation="VLEIDGASNNSVDDVRTIRENISYGPVKSKRKAYIIDEVHMLSK
                     SAFNALLKTLEEPPTHTVFILATTDPQKIPMTVLSRCQRYDLRRLGTADIVRQLEKIL
                     TAEKVSFDREALFLIAREGDGSMRDAQTLVEQMLAFGERTITGTVVTTILGISDRALI
                     RGIVEAVARRDADAAVTRVREIYASGKALEKAARDVLFTLHQLVLASSLSDDRFLEAS
                     DEERRWVRESARLLTPADWLRLFDLWSKESDRILRSEYPLLLFEISVVLAASLPVMTD
                     LRTLLDTLRDAPVPAARSHEPAPAIQNEPPRGAPKTTQPTARTAEPVPPPSPTTTPKA
                     EPIAPPTVTGWRDFVERLAQEEPMTGGALKTVTALEKAGTVTVQIPQATKALLDPKKE
                     MLTARFAELTGGRKLRFADEMSEGTSVAQQETKEEKEKNAKIREEVRNDPTVKKTEQA
                     GFDFKKSSPT"
     gene            1380..>2215
                     /locus_tag="PKH10_11940"
     CDS             1380..>2215
                     /locus_tag="PKH10_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28872.1"
                     /translation="MIRRIPHAIVAVALFGLFFLWGTRGKPWLFFTAFWPTIHLLYGG
                     VAAAIALCLALRPDLIRLLRDRLVRHRYFIFPPITAVATVLISWLVFDNIPHVIDAAH
                     FLWSARLMLDGSLSLPAGDLYEFFDVTFMVRDGGRHYSLFLPGFSLALAPFAALGISW
                     LLVPLASGLSVWLTGRIADRFFNGRVSCLAMALCTFSSFYLFMGASFMTHNFNLLLLL
                     TAVWLVTRDHRDGRTLFAASAAIALSLFMRPQNALFAYLPLGIYLLLKRTTFLRLALF
                     AIP"
BASE COUNT          404 a          770 c          628 g          413 t
ORIGIN      
        1 gtgctcgaga tagacggcgc gtcgaacaac tcggtggacg atgtccgcac catccgcgag
       61 aacatctcct acggcccggt caaatcgaaa cgcaaggcct acatcatcga cgaggtacac
      121 atgctctcga agtcggcctt caacgcgttg ctcaaaacgc ttgaagaacc gccgacccac
      181 accgtattca tactcgcgac caccgacccg cagaagatac cgatgacggt gctcagccgt
      241 tgccagcgtt acgacctgcg ccggctcggg accgccgaca tcgtgcgaca actcgagaag
      301 atactgacgg cggagaaggt atcgttcgac cgcgaggcgc tgttcctcat cgcgcgggaa
      361 ggcgacggca gcatgcgcga cgcccagacg ctcgtcgaac agatgctcgc cttcggggag
      421 cggaccatca ccggcacggt ggtgacgacc atccttggca tcagcgaccg cgcgctcatc
      481 cgcgggatcg tcgaagctgt cgcgcgccgg gatgccgacg cggcggtcac cagggtacgc
      541 gagatatatg cgagcggcaa ggcactcgag aaggcggcac gcgatgtcct cttcaccctg
      601 caccaactcg tgctcgcctc ctcgctttcg gatgaccggt tcctcgaggc ctccgacgag
      661 gagcgacgct gggtccgcga aagtgcacga ctccttaccc ccgccgactg gctccgcctg
      721 ttcgacctct ggtccaagga gagcgatcgt atcctgcgga gcgaatatcc cctactcctg
      781 ttcgagatat cggtcgtgct cgccgcatcg ctcccggtga tgaccgacct gcgtacactg
      841 ctcgacacgc tccgtgacgc accggtgccg gccgccagat cgcatgagcc ggcccccgcg
      901 atacaaaacg agccgccccg gggcgcgcca aagaccacgc aaccgaccgc gagaacagcg
      961 gagccggtgc cgcccccctc accgacgaca acgccgaaag ccgagccgat cgcgccaccc
     1021 accgtcaccg gctggcgcga tttcgtggaa cggctggcgc aggaggaacc gatgaccggt
     1081 ggcgccctga aaacggtgac ggcgctcgag aaggccggca cggtcaccgt gcagataccg
     1141 caggcgacca aggcgctact cgaccccaag aaagagatgc tcaccgcccg cttcgccgaa
     1201 cttaccggcg gcaggaaact gcgattcgcc gacgagatgt cggaggggac ctcggtcgcc
     1261 caacaggaaa caaaagagga gaaggagaag aacgcgaaga tacgcgaaga ggtacggaac
     1321 gacccgaccg tcaagaagac cgaacaagcc ggattcgatt ttaaaaagtc atcgccgaca
     1381 tgattcgcag gataccgcat gcgatcgtcg cggtcgctct gttcggtctt tttttcctct
     1441 gggggacccg cggcaaaccg tggctcttct tcaccgcctt ctggccgacg atccacctgt
     1501 tgtacggcgg cgtcgccgct gcgatcgccc tctgtttggc gctccgtccc gacctgattc
     1561 gtcttttgcg tgaccggttg gtcaggcacc gttactttat tttccccccg atcactgcag
     1621 ttgccacggt gctcatctcg tggctcgttt tcgataacat cccccatgtc atagatgccg
     1681 cccatttcct ctggtccgcg cggctcatgc tcgacggctc gctctcgctc ccggcaggcg
     1741 acctgtacga attcttcgat gtcaccttca tggtgcgcga cggcggccgg cactactcgc
     1801 tgttcctccc cggattctcg ctcgcgctcg ccccgttcgc ggcgctgggg atctcgtggc
     1861 tcctcgtccc gctcgcaagc ggcctttcgg tctggctgac cggccgtatc gccgaccggt
     1921 tcttcaacgg acgggtgagt tgcctcgcga tggcgctctg caccttttcc tccttttatc
     1981 tcttcatggg cgccagtttc atgacccaca acttcaatct gctcctcctc ctgaccgccg
     2041 tctggctcgt gacgcgggac caccgtgacg gtcgcacgct ttttgccgca tcggccgcca
     2101 tagccctgtc gctgttcatg cgaccgcaga acgccctgtt cgcctacctg ccgctcggca
     2161 tctatcttct tctcaagcgg acgacgttcc tccgtcttgc cctcttcgcg atacc
//
LOCUS       DAOQUE010000471         2202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_62118_length_2202_cov_4.361900, whole genome shotgun sequence.
ACCESSION   DAOQUE010000471 DAOQUE010000000
VERSION     DAOQUE010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62118_length_2202_cov_4.361900"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="PKH10_11945"
     CDS             <1..1568
                     /locus_tag="PKH10_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28873.1"
                     /translation="VRLNYKQLAPEIYKEMLSGDLKDEGSYAIEFCTNDPAKEASPTT
                     CKEGTSYKVQIYRYPNLPPKPFIGLKYSSPIGGYKDHRVIKDWVNMEVNLSCPRKEGA
                     DEATCNKIISDTVSGPDTISTNDFRDQCCAANWEEQYCLKYRWEMVETPTPLAPETHI
                     GLDNIPLGGEGNWYEACGELDPTLASFKGYMITPTPEGKYYKVSIQAVTIDKQTQLES
                     DITEKIDAPNIIPAARVMVQLTWKEGLQTRAELDRQEGVAVDLDLHLIKKKGMDACRN
                     EAGVDGLMCTVMSRPGLPIDTPTHDDCHWNDLGLNGEYVFTDCNGEFKTIGWHAAFDL
                     DNRWGGGDYTNPETINLGPITDENPKDGKPDVNPLDDEYLVMVNYNNCQNLQSGGDEA
                     PCQDGGEEYNVHAKVEIFVDGKVAPRDGKPDDNPLNRTKFVIRPREWVVIGTFTWDGT
                     LTPSTPGWVGDAVVHDLPTTHEKVCQFDSTHCRNVTIWDYATYETWVKNPTPVDDEFG
                     GGSGDCYDYGSRQ"
     gene            1592..>2202
                     /locus_tag="PKH10_11950"
     CDS             1592..>2202
                     /locus_tag="PKH10_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28874.1"
                     /translation="MTRDTPLETFLLMKNGSLLSFALFLTLCAATLSAKEQYRYEVLV
                     AGVPSGEVIDTWEEAMIDGRCVVKSESAMRIVLSRGGFDLAMSTRTVAEADCVTYRPL
                     SLSVSRDEGGGPILTTARREGDLLISKTEKNGVSEKGILKLDGASVFFGMLFRRYPVE
                     YFLKKGEVSAISEEGLTVRDLSFAGRRAGKDVVVDMEYAGVPIT"
BASE COUNT          498 a          627 c          679 g          398 t
ORIGIN      
        1 aagttagact gaactataag cagctggccc ccgagatata caaggagatg ctgagcgggg
       61 atctcaagga cgagggcagt tacgcgatag agttctgcac caacgacccc gcgaaggagg
      121 cctcgccgac cacttgcaag gagggcacct cctacaaggt gcagatatac cgctatccca
      181 acctgccgcc caagccgttc atcggtctga aatattcgag cccgatcggc gggtacaagg
      241 accaccgggt catcaaggac tgggtcaaca tggaggtcaa tctctcctgt ccgcgtaaag
      301 aaggggccga cgaggcgacc tgcaacaaga taatatccga caccgtctcc ggtcccgaca
      361 ccatctcgac gaacgatttc cgcgatcagt gttgcgccgc gaactgggag gaacagtact
      421 gcctcaagta tcgctgggag atggtcgaga ccccgacgcc gcttgctccc gagacccaca
      481 tcggtctcga caacatcccg cttggcggcg agggcaactg gtacgaggcc tgcggcgaac
      541 ttgatccgac actcgcctcg ttcaagggct acatgatcac cccgacgccc gaaggcaagt
      601 actacaaggt cagcatacag gcggtcacga tagacaagca gacccagctg gagagcgata
      661 tcaccgaaaa gatagacgct cccaacatca tcccggcggc ccgcgtcatg gtgcagttga
      721 cctggaagga gggtctgcag acccgcgccg aacttgaccg gcaggaaggg gtcgcggtcg
      781 atctcgatct gcacctcatc aagaagaagg gcatggatgc ctgccggaac gaggcggggg
      841 tcgacggcct catgtgcacc gtcatgtcgc gtccgggcct gccgatagac acgccgaccc
      901 atgacgactg ccactggaac gatctgggcc tgaacggcga gtatgtcttc accgactgca
      961 acggcgagtt caagaccatt ggttggcatg ccgcgttcga cctcgacaac cggtggggcg
     1021 gcggtgatta caccaacccc gagacgataa accttggccc gataaccgac gagaacccca
     1081 aggatggcaa accagatgtg aacccgcttg acgacgaata tctggtcatg gtgaactaca
     1141 acaattgcca gaatctccag tcgggtggag atgaggcgcc gtgtcaggat ggcggagagg
     1201 aatacaatgt ccacgcgaag gtcgagatat tcgtggacgg caaggtggcg ccgcgcgacg
     1261 gtaagccgga cgacaacccg ctgaacagaa cgaaatttgt tattcgtccc cgtgagtggg
     1321 tcgtcatcgg gaccttcacc tgggacggta cccttacccc ctcgacgccg ggctgggtgg
     1381 gtgacgcggt cgtacacgat ctcccgacga cccatgaaaa ggtctgtcag ttcgattcga
     1441 cccattgtcg caatgtgacc atatgggatt acgccacgta cgagacctgg gtgaagaacc
     1501 ccacaccggt cgatgacgag ttcggcggcg ggagcggcga ctgctacgat tacggctctc
     1561 ggcagtgatc catcggcgtc ccgcggtcga gatgacgcgg gatacccctc ttgagacatt
     1621 cctcctcatg aagaacggtt cgcttctctc ctttgcgctc ttcctcacgc tgtgcgccgc
     1681 gaccttgagc gccaaggaac agtaccgtta tgaggtgctc gtagccggcg tgccgtccgg
     1741 ggaggtgatc gacacctggg aagaggcgat gatcgacgga cgctgcgtgg tgaaaagcga
     1801 gagcgcgatg cggatcgtcc tttcccgcgg agggttcgat ctggccatga gtacccggac
     1861 ggtggccgag gccgattgcg tgacctaccg accgctttcg ctttcggtga gccgcgatga
     1921 ggggggcggg cctatcctta cgacggcccg gcgcgagggc gatctcctga tatcgaagac
     1981 cgaaaagaat ggcgtatccg aaaaggggat acttaaactc gacggcgcat cggtattctt
     2041 cggcatgttg ttccgcaggt atccggtgga atattttctc aaaaagggag aggtttcggc
     2101 gatatcggag gagggactta cggtacgcga tctttccttc gcggggcgtc gtgcaggaaa
     2161 agatgttgtc gtcgacatgg agtatgcggg agtgccgata ac
//
LOCUS       DAOQUE010000472         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_62696_length_2189_cov_3.495314, whole genome shotgun sequence.
ACCESSION   DAOQUE010000472 DAOQUE010000000
VERSION     DAOQUE010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62696_length_2189_cov_3.495314"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /gene="greA"
                     /locus_tag="PKH10_11955"
     CDS             complement(<1..294)
                     /gene="greA"
                     /locus_tag="PKH10_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNT28875.1"
                     /translation="MATYPITTRGYAAIKKELDHLKKVDRPAIIKAIAEARSHGDLSE
                     NAEYHAAKERQGFIEERIKNLEGIVATAEVIDPAKLSGDRVLFAKWAGTELKID"
     gene            complement(523..1947)
                     /locus_tag="PKH10_11960"
     CDS             complement(523..1947)
                     /locus_tag="PKH10_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HNT28876.1"
                     /translation="MTALLVGFIVYLVLMLTIGAITWRLNRSTTDYYLGGRTLGPWLT
                     AFSERTSGESAWLLLGLPGAALALGLIEVWTAVGCVSGIMFSWYVIAERLRVETERYN
                     AITLPEYLAARFGRHARSIRSLAMLIIVFFFTFYLAAQMNGAGKVLNVTFDLPQRSGI
                     LIGAAIIVLYTMMGGFLAVVWTDLVQAILMIATLVILPVVALFELSAQGISLGDAMTN
                     AGTVASLTGGKAGWAGAAAVIGGLSWGLGYMGQPHLLTKFMAIRTPQEIRVGRRIAFA
                     WAVPAFTGAIIIGLAALALHGGAFYGDRERVMPALATELFPGWLAGILISGAIAAMMS
                     TADSQLLVISSAVIEDFYRKTLKRELSDRAMVTLSRLITLTVGIAGILLALYSDKLIF
                     AMVSYAWSGLGSSFGPAILLTLLWKKTSGPGIIAGMLTGAITTVVWSSIPALDGLITA
                     RLVSFVLAVAAIIVVSSGRDEKAL"
     gene            complement(1959..>2189)
                     /locus_tag="PKH10_11965"
     CDS             complement(1959..>2189)
                     /locus_tag="PKH10_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28877.1"
                     /translation="HGVKGWVGGYPIPEVHLYGGIGTLHPVHFSPFVGVDWRVTDLLA
                     LSLTMEYHAPADETPVDFDTSIFGSLGATFYF"
BASE COUNT          453 a          644 c          710 g          382 t
ORIGIN      
        1 gtcgatcttc aattccgtgc cggcccattt ggcgaacagc acccggtcac ccgacagttt
       61 cgccggatct atcacctcgg cggtggcgac gatgccttcg aggttcttta tccgttcctc
      121 gatgaagccc tgccgttcct tggcggcgtg atattcggcg ttctccgaga ggtcgccgtg
      181 cgatcgggcc tcggcgatcg ccttgatgat ggcggggcga tcgaccttct tgagatggtc
      241 gagttccttt ttgatcgcgg catatccgcg ggtcgtgatc gggtaggtgg ccatcgcgct
      301 tttctccttc agcagacagg aagaacaggt gtccccatca taaccgacca cgaccgataa
      361 tcaagaaaag agactatgtt tccgatatca cggtcgaaaa aagatgggaa gcgataatgc
      421 tgttccgggg atgtccgatg cccgtcagag ggcgtcgatc gttctgtcca cctctcgtcc
      481 ctccctcgcg gacctctcga tcccggggga gggatgtttt ttttagaggg ctttttcgtc
      541 ccggccggat gaaactacga tgatggccgc gacagcgaga acgaaactga cgagccgcgc
      601 cgtgatgagg ccgtcgagcg cggggatgct cgaccagacg acggtggtta tcgcaccggt
      661 cagcattccg gcgatgatgc cggggccgga ggtctttttc cacaagagtg tgagcagtat
      721 cgcagggccg aacgacgatc cgagccccga ccatgcatag gagaccatag cgaatatcaa
      781 tttgtcggaa tacagcgcga gcaggatgcc ggcgataccg accgtgagcg tgatgagccg
      841 tgagagcgtc accatcgccc ggtcggaaag ttcccgtttc agcgtcttgc ggtagaaatc
      901 ctcgatgacc gcggatgata tgacgagcag ttggctgtcg gcggtcgaca tcatcgccgc
      961 gatggcgccc gatatgagga tgccggcgag ccaaccgggg aagagttcgg tcgcgagcgc
     1021 cggcatcacc cgttcgcggt cgccgtaaaa ggcgccgccg tgcagggcga gcgccgcgag
     1081 tccgatgatg atggcgccgg tgaaggcggg gacggcccac gcgaaggcga tgcggcgacc
     1141 gacccgtatc tcctgcgggg tccggatcgc catgaacttg gtgagcagat gcggctgtcc
     1201 catgtagcca agaccccacg agagaccgcc gatcaccgcc gcggcgcccg cccagccggc
     1261 tttgccgccg gtaaggctgg cgaccgtccc ggcgttcgtc atcgcgtcgc cgagcgatat
     1321 cccctgcgcc gagagttcaa aaagcgccac gacaggcagt atgaccaggg tcgctatcat
     1381 gaggatcgcc tgtaccagat cggtccagac gaccgccaga aagccgccca tcatggtgta
     1441 gaggacgatt atcgctgcgc cgatgaggat accgctccgc tgggggagat cgaaggtgac
     1501 attgagcacc ttgcccgcgc cgttcatctg ggccgcgagg tagaaggtga agaaaaagac
     1561 gatgatgagc atcgcaagcg accgtatcga ccgggcgtgc cgaccgaacc gggcggcgag
     1621 gtattcgggc agggtgatgg cgttgtaccg ttcggtctcg acgcgcagtc tctcggcgat
     1681 gacataccac gagaacatga tgccggaaac acaaccgacg gcggtccaca cctcgatgag
     1741 gccgagcgcg agcgccgcac cgggaagccc gagcaagagc cacgccgatt cgccgcttgt
     1801 ccgttcggaa aaggcggtaa gccacgggcc gagcgtacgg ccgccgagat agtagtcggt
     1861 cgtcgaccgg ttaagccgcc aggtgatggc cccgatggtc agcatcagga caagatagac
     1921 gatgaaaccg acaagaagcg ccgtcatgac ttactccctc aaaaataaaa ggtagcgccc
     1981 agtgagccga atatggaggt gtcgaagtcg accggcgtct cgtcggcggg cgcgtggtat
     2041 tccatggtca gggaaagggc gaggagatcc gtgacccgcc aatcgacccc gacgaacggg
     2101 gagaaatgga ccggatgaag ggtgccgatg ccgccgtaca ggtgcacctc ggggatcgga
     2161 tacccgccca cccagccctt caccccgtg
//
LOCUS       DAOQUE010000473         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_62738_length_2188_cov_4.202063, whole genome shotgun sequence.
ACCESSION   DAOQUE010000473 DAOQUE010000000
VERSION     DAOQUE010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62738_length_2188_cov_4.202063"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PKH10_11970"
     CDS             complement(<1..490)
                     /locus_tag="PKH10_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28878.1"
                     /translation="MKKNLFVIMFLAMVLTAACGDDNTGPTGPDEGSACDGSTTMCVG
                     EVYYWCNNNAWALIDCNATGKVCDVEDGCVASGTDNNTGTDDNIPVNCGNNTTDTGEV
                     CDGDAKDCTTLGGGYTGGWATCKADCSGYDTSTCVGGTTDADTVNDNAMPDNDAVSPT
                     GKT"
     gene            complement(597..>2188)
                     /locus_tag="PKH10_11975"
     CDS             complement(597..>2188)
                     /locus_tag="PKH10_11975"
                     /inference="COORDINATES: protein motif:HMM:NF025316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HNT28879.1"
                     /translation="GNAKVLDKGTIMYKQNLLEVGDPSGTCQGDSGGPFFTTQNGQLV
                     VSGVTSFGGQTCYADRDGYDTWVLKYYSWIEGIVQQFTGHGLLGSGVCGDGNDYCSQG
                     QSKDCGQIDIRYQSGTTAYCNTGCSYWDVSVCIPVCGDGELLSPELCESGDTADCGTL
                     GQFLTGTTAPCRDDCLGYNTAYCTATFCGDGVKEGNEFCDTQLTSCETLGDYNKYSYA
                     RCNADCTGFDANDCVNQTIVCGNGNVDYLEQCDDGNTTSGDGCSSKCKIEGATVVCGN
                     GIKEGAEECDDGNTVNGDGCSAGCVTEAMAVCGNGVREWGEECDDGNTVNGDGCSAGC
                     LTESPYAVCGNGTLETGEQCDDGNTVGGDGCSAACLIETGAVCGNGTVETGEQCDDGN
                     TTNGDGCSAACLTEDPNAVCGNGTLEQGEQCDDGNMKPGDGCNPDCTLPATSGGTCGN
                     GIKEAYEQCDDGNMIPGDGCNPDCTIPGVAGTCGNGVKEWGEECDDGNLSAGDTCNPD
                     CTKPTTTTPRGSSGGCSLILL"
BASE COUNT          320 a          719 c          676 g          473 t
ORIGIN      
        1 aggtcttgcc cgtcgggctc acggcgtcgt tgtcgggcat ggcgttgtcg ttgacggtgt
       61 cggcgtcggt cgtgccgccc acgcaggtgc tcgtgtcgta gccggagcag tcggccttgc
      121 aggtggccca gccgccggtg tagccgccgc cgagcgtcgt gcagtccttc gcgtcgccgt
      181 cgcacacttc gccggtgtcg gtggtgttgt tgccgcagtt caccgggatg ttgtcatcgg
      241 tgccggtgtt gttgtcggtc ccggacgcta cgcagccatc ctcgacatcg cacaccttgc
      301 cggtcgcgtt gcagtcgatg agcgcccacg cgttgttgtt gcaccagtag tacacttcgc
      361 cgacgcacat ggtcgtcgag ccgtcacagg cggagccctc gtcagggccg gtcggaccgg
      421 tgttgtcgtc gccgcaggcc gcggtgagca ccatcgcgag gaacatgatc acgaaaaggt
      481 tcttcttcat ctttgatacc tccatacagg atagtaaagg gttacaaccc gctaaacatc
      541 gccgatttta tgagccgttg gtcgctaaag tcaagtattt tctcctttcg tcccgttcac
      601 aggaggatga gcgagcaccc gcccgatgaa ccgcgcgggg tggtcgtcgt cggtttggtg
      661 cagtcgggat tgcaggtgtc gcccgccgac aggttgccgt cgtcgcactc ctcgccccac
      721 tccttgacgc cgttgccgca ggtccccgcc actcccggta tcgtgcagtc ggggttacag
      781 ccgtcgcccg gtatcatgtt gccgtcgtcg cactgctcgt acgcttcctt tatgccgttg
      841 ccgcaggtgc cgcccgaggt cgccgggagg gtgcaatcgg ggttacagcc gtcgcccggc
      901 ttcatgttgc cgtcgtcgca ctgttcgccc tgttcaagcg tcccgttgcc gcacacggcg
      961 ttcggatctt cggtgagaca ggccgccgaa cagccgtcgc cgttggtggt attgccgtcg
     1021 tcgcactgtt cgccggtctc cacggtgccg ttgccgcaga cggcgcccgt ttcgatgaga
     1081 caggccgccg aacagccgtc gccgccgacc gtattgccgt catcgcactg ttcgcccgtt
     1141 tcgagggtcc cgttgccgca gaccgcgtaa ggcgattcgg taagacagcc cgccgaacag
     1201 ccgtcgccgt tgacggtgtt gccgtcgtcg cattcttcgc cccactccct gacgccgttg
     1261 ccgcagaccg ccatcgcctc ggtgacgcac ccggccgaac aaccgtcgcc gttgacggtg
     1321 ttcccgtcat cgcattcctc ggccccctct ttgatgccgt tgccgcacac cacggtcgcc
     1381 ccttcgatct tgcatttcga cgaacagccg tcgcccgagg tggtgttgcc gtcgtcgcac
     1441 tgttcgaggt aatcgacatt cccgttgccg cagacgatcg tctgattcac gcagtcgttg
     1501 gcgtcgaatc cggtgcagtc ggcgttacag cgggcgtaac tgtatttgtt gtagtcgccg
     1561 agcgtttcac aactggtcaa ctgggtgtcg cagaactcgt tcccctcctt gaccccgtcg
     1621 ccgcagaagg tcgcggtgca gtaggcggtg ttgtagccga ggcagtcgtc gcggcacggc
     1681 gcggttgtcc cggtgaggaa ctggccgagc gtcccgcagt cggcggtgtc gcccgactcg
     1741 cacagttccg gtgagagtag ttcgccgtcg ccgcagaccg gtatgcagac cgacacatcc
     1801 cagtaggaac agccggtgtt gcagtaagcg gtggtccccg actggtaccg gatgtctatc
     1861 tgaccgcagt ccttgctctg tccctgagaa cagtagtcgt tcccgtcgcc gcagacgccg
     1921 gagcccaaga gtccgtggcc ggtgaactgc tggacgatgc cctctatcca tgagtaatat
     1981 ttgagcaccc aggtgtcgta tccgtcacgg tcggcgtaac aggtctgccc gccgaacgag
     2041 gtgacgcccg acaccaccag ttgcccgttc tgcgtcgtga agaacgggcc gcccgagtcg
     2101 ccctgacagg tgcccgacgg gtcgcccacc tcgaggaggt tctgcttgta catgatggtc
     2161 cctttatcca gcaccttggc gttacccc
//
LOCUS       DAOQUE010000474         2182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_62999_length_2182_cov_5.577809, whole genome shotgun sequence.
ACCESSION   DAOQUE010000474 DAOQUE010000000
VERSION     DAOQUE010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62999_length_2182_cov_5.577809"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /gene="rho"
                     /locus_tag="PKH10_11980"
     CDS             <1..448
                     /gene="rho"
                     /locus_tag="PKH10_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173899.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNT28880.1"
                     /translation="ARAYNTVVPPSGKILSGGVDANALQKPKRFFGAARNIEEGGSLT
                     IIATALIDTGSRMDEVIFEEFKGTGNMEIHLDRKLMEKRIFPCLDINKSATRKEELLL
                     EKDILNRVWILRKLLHPLNVIDSMEFLLDKMGRTKTNDDFLKAMNA"
     gene            complement(456..1028)
                     /locus_tag="PKH10_11985"
     CDS             complement(456..1028)
                     /locus_tag="PKH10_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28881.1"
                     /translation="MRRSFWLLTLVLFTGCGREEIDAADRAATAAWEELWDRAGERIT
                     IMRELARDLCPTAPKEATSLCGAVERSLREEDAEGKGARPAEHDRDAVGRAGKRYREW
                     GYHAESLRSLRKGPVGEDPAAAALIDRVLAGNERALTARDRYNDAVLAYNRALATFPE
                     SVTNNMLLHLEPRASFAENRQFRRDMPARR"
     gene            1180..1587
                     /locus_tag="PKH10_11990"
     CDS             1180..1587
                     /locus_tag="PKH10_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT28882.1"
                     /translation="MKIERLIDLRLSMERVIAHYVARMSEEYIEDEKGLHVISPAVDS
                     YLDGDRQILFLETPALDASTIEISHHNGMLVFRGAKKQPDRRGRRYLHMERATGDYLK
                     VIPIEGKEDAIAGIEHSYRFGVVKIVVSFSETA"
     gene            1584..1970
                     /locus_tag="PKH10_11995"
     CDS             1584..1970
                     /locus_tag="PKH10_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28883.1"
                     /translation="MRSVAPLFPFLFFCLIVSAEGKDQKTEKPAEATVSFEKKEGGVD
                     YTIAIQPPFKMNREAPFKFELRRDANDTLKKVALDEFAKDGKSETYRYRSVMNEKQIY
                     YWFIACKYKGEEVVACKTFSAKQDLP"
BASE COUNT          490 a          702 c          608 g          382 t
ORIGIN      
        1 cgcccgcgcc tacaacaccg tcgttccgcc gtcgggcaag atcctgtcgg gcggcgtcga
       61 cgccaacgcc ctgcagaagc ccaaacgctt cttcggcgcc gcgcgcaaca tcgaagaagg
      121 cggttcgctg accatcatcg cgaccgcgct catcgatacc ggttcacgca tggacgaggt
      181 catcttcgaa gagttcaagg gcaccggcaa catggagatc cacctcgacc ggaaactgat
      241 ggaaaaacgc atattcccgt gcctcgatat caacaagtcg gccacgagaa aagaggaact
      301 cctgctcgaa aaggacatcc tcaatcgcgt gtggatactg cgcaaactgc tccacccgct
      361 caatgtcatc gattcgatgg aattcctgct cgacaagatg ggccgtacca agaccaacga
      421 cgacttcctg aaggcgatga acgcctgacg gacctctatc tccgggccgg catatccctc
      481 cggaactgtc ggttctcggc gaacgacgcc cgcggctcaa gatgcaacag catattattg
      541 gtcacgcttt cgggaaaggt ggcgagcgcc cgattgtagg ccagcaccgc gtcgttgtaa
      601 cggtcgcgcg ccgtgagcgc ccgttcgttg cccgcgagga cgcgatcgat cagcgccgcc
      661 gccgccgggt cctcaccgac cggaccttta cgcagagagc gcaacgattc ggcatgatac
      721 ccccactccc ggtaccgttt cccggcccga ccgaccgcat cgcgatcatg ctccgccgga
      781 cgcgcccctt tcccctccgc gtcctcttca cggagcgacc gctcgaccgc gccacacagc
      841 gatgtcgcct ctttcggggc ggtcggacag agatcccgcg cgagttcccg catgatcgtg
      901 atccgctccc cggcgcgatc ccaaagttcc tcccatgccg cggtcgccgc gcggtcggcg
      961 gcatctatct cttcgcgacc gcacccggtg aacaggacca gcgtcaggag ccagaacgaa
     1021 cggcgcatat cggacctcca tgaaaagaac gcgcccatgg taccgccacg cctcgataag
     1081 gtcaagaaag tgctcctccc gcagaccgtg gccgccgttt ctttgacttt ttgttttttc
     1141 tattcatata atggggtccg ttatggaggt tagcgtccca tgaagataga gcggctcatc
     1201 gacctgcgtc tcagcatgga acgggtcatc gcccactatg tcgcccgcat gagcgaggaa
     1261 tacatagaag acgaaaaggg gctccatgtc atctcgcccg ccgtagacag ttatctcgac
     1321 ggcgaccgcc agatactgtt cctcgagacc ccggccctcg atgcctcgac catagagata
     1381 tcgcaccaca acgggatgct cgtcttccgc ggcgccaaga aacagcccga ccggcgcggt
     1441 cgccgttacc tccacatgga gcgtgcgacc ggcgattacc tcaaggtgat accgatcgag
     1501 gggaaggagg atgccatcgc cggcatcgag cattcctatc gtttcggcgt cgtcaagatc
     1561 gtcgtctcct tctcggagac ggcatgagat ccgtcgcacc ccttttcccg ttccttttct
     1621 tctgcctgat cgtatcagcc gaggggaagg atcagaaaac ggaaaaaccg gccgaagcga
     1681 cggtgagttt cgagaagaag gagggcggtg tcgattacac cattgcgata cagccgccgt
     1741 tcaagatgaa ccgcgaggcc cccttcaagt tcgaactgcg tcgcgacgcg aacgacactc
     1801 tgaagaaggt cgcgctcgac gagttcgcga aggatgggaa gagcgagacc taccggtacc
     1861 gctccgtcat gaacgagaaa cagatatatt attggttcat cgcctgcaaa tacaagggcg
     1921 aggaagtggt cgcctgcaag accttctcgg caaaacagga cctgccgtga acggcgcagc
     1981 cacccgcgag acattataag cctaccccat acgaggtgaa agcatgggca ttttgaaagc
     2041 gatctccgaa gcagtgaagg aaaaggcgaa agaggagatc atccggtacc tgaaaaaggt
     2101 cgccgacgag aacggatcga gaccgccgca cccccgcgac cggcaatcgg aacgccccga
     2161 ccgtcacgcg gaatcacggc gc
//
LOCUS       DAOQUE010000475         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_63061_length_2181_cov_3.739417, whole genome shotgun sequence.
ACCESSION   DAOQUE010000475 DAOQUE010000000
VERSION     DAOQUE010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63061_length_2181_cov_3.739417"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..495
                     /gene="rpmE"
                     /locus_tag="PKH10_12000"
     CDS             271..495
                     /gene="rpmE"
                     /locus_tag="PKH10_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNT28884.1"
                     /translation="MAKQGIHPKLHLITISCSCGNSFQTMSTKDGGYTVEICSACHPF
                     YSGKQKIVDTAGRIEKFKQKYKKADYSGKK"
     gene            589..1629
                     /locus_tag="PKH10_12005"
     CDS             589..1629
                     /locus_tag="PKH10_12005"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNT28885.1"
                     /translation="MTEKRILNAGGQAVIEGVMMRTPDYFVIAVRKPDGTIKVRSRRW
                     ENLFPGIVKKPFLRGMFVLYEAMYNGMQAITWSANESVEEGKEEDKLTKTGTVMTILA
                     AFALAMLLFVALPHGAAYFLEKGIRGETSINSMLFHFIDGVVKVIIFSLYLWGISKMA
                     EVRRLFSYHGAEHKAITTWEAGEELIPENARKYPTAHPRCGTSFMITVILISIIVFTV
                     VFAFVPPITGSKLLDNLAYIFIKILLIFPIGGVSYELQKLVSRFPKSRVLAVMIAPGL
                     WYQRITTTEPAPDVLEIGLVSLTTALDLQEKKAVLPLDGAEEIFHDFAEFRAKMSGAL
                     NPAPPEGTPCSI"
     gene            1617..>2181
                     /locus_tag="PKH10_12010"
     CDS             1617..>2181
                     /locus_tag="PKH10_12010"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNT28886.1"
                     /translation="MLDIKDKLEELYKKYQDLEGELSRPDVVADLKRFKKLSQEYAHL
                     KEIAQKFLDWKRISAEIVGHKDLLRSEKDADILTLIKDELPELEERYDLLREELRVLL
                     LPHDPMDDKNAILEIRAGTGGDEAALFAGEVFRMYSRYAELRGWKVEVISSSAQEGGG
                     MKEVVAEVKGSGVYGRLKYEGGVHRVQR"
BASE COUNT          493 a          600 c          621 g          467 t
ORIGIN      
        1 acgaatgtaa gatcggcgac ctggtctgct acgacaatcc ttcctgcaac accggttccg
       61 cgcttctcct cctgaatgcg aaagaatgta tcccggcggg cgatgtttgc tcggagaacg
      121 atacctgctg ttccggccag ccctgcatcc aggggtattg ctactagtcc gttaccccgt
      181 cgaggggtca tctaaaaaac ttcttgcttt ttcatcggtc atggctaata tccaaacccg
      241 tttttggcat tgtgaaaaaa ggagcgtatg atggcgaaac aaggcatcca cccgaaactg
      301 catctcatca cgatatcctg ctcgtgcggg aattcattcc agacgatgtc gacgaaggac
      361 ggcgggtaca cggtcgagat atgctcggcg tgccatccct tctacagcgg caagcagaag
      421 atcgtcgaca ccgccggccg catcgagaag ttcaagcaga aatacaagaa ggccgactac
      481 tccggcaaga agtagatcgc tcgtgtcgtt ctgctctctt acgatgcatc cttgcccgct
      541 tttcgcccgt tagatagatt tcccttataa attcatcgga gcatcctcat gaccgagaag
      601 cgcatactca atgccggagg gcaggccgtt atcgaagggg tgatgatgcg gacccccgat
      661 tacttcgtca tcgcggtccg caaacccgac ggcaccatca aggtccggag ccgccgttgg
      721 gagaacctct ttcccggtat cgtcaaaaag cctttcctgc gcggcatgtt cgtcctttac
      781 gaggcgatgt acaacgggat gcaggcgatc acctggtcgg ccaacgagtc ggtcgaagag
      841 gggaaggagg aggataaact gacgaagacc ggtaccgtca tgacgatact cgccgccttt
      901 gcgctcgcga tgctgttgtt cgttgctctc ccccacggtg ccgcttactt ccttgaaaaa
      961 ggtatccgtg gcgagacctc tatcaattcc atgctcttcc acttcatcga cggcgtggtg
     1021 aaggtcatca tcttctcgct ctatctctgg ggcatctcga agatggccga ggtgagacgc
     1081 cttttttcct atcacggcgc cgagcacaag gcgataacga cgtgggaggc gggcgaagaa
     1141 ctgatccccg agaacgcccg aaaatatccg acggcccacc cgcgttgcgg caccagtttc
     1201 atgatcaccg tcatactcat ctcgataatc gtgttcaccg tcgtattcgc tttcgtgccg
     1261 ccgatcaccg gctcaaaact gcttgataat ctcgcctaca tcttcatcaa gatactgctc
     1321 atttttccca tcggcggagt gagttacgaa ctgcagaaac tggtgtcgcg attcccgaag
     1381 agccgggtgc ttgcggtgat gatcgctccc ggcctgtggt atcagcgcat cacgacgacc
     1441 gaacccgccc ccgatgtgct cgagatcggg ctggtatcgc ttaccacggc gcttgacctg
     1501 caggagaaaa aggcggtcct gccgctcgac ggggcggagg agatattcca cgactttgcc
     1561 gagttccgcg cgaagatgag cggtgcgctc aacccggcgc cgccggaggg gacgccgtgc
     1621 tcgatataaa agacaaactg gaagaactct acaagaaata tcaggatctc gaaggggaat
     1681 tgtctcggcc cgatgtggtg gccgatctca agcggttcaa gaaattgtcc caggagtacg
     1741 cgcacctcaa ggagatcgcg cagaaattcc tcgattggaa gaggatatct gctgagatcg
     1801 ttgggcacaa ggacctgttg aggagcgaaa aagacgccga tatcctgacg ctcatcaaag
     1861 atgagttgcc cgaactggag gagagatacg atctcctcag ggaggaattg cgcgtcctgc
     1921 tcttgccgca tgatccgatg gacgacaaga acgccatcct cgagatacgg gccggtaccg
     1981 gtggtgatga ggcggcgctt tttgcgggcg aggtcttccg gatgtacagc cggtatgccg
     2041 aactcagggg ctggaaggta gaagtcatct cctcgagcgc tcaggaggga gggggcatga
     2101 aggaggtcgt cgctgaagtg aagggttctg gcgtttatgg ccggctcaaa tacgaaggag
     2161 gtgttcaccg tgtccaacgg g
//
LOCUS       DAOQUE010000476         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_63252_length_2177_cov_4.056079, whole genome shotgun sequence.
ACCESSION   DAOQUE010000476 DAOQUE010000000
VERSION     DAOQUE010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63252_length_2177_cov_4.056079"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKH10_12015"
     CDS             <1..217
                     /locus_tag="PKH10_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28887.1"
                     /translation="TGEHAQLAPDASLRIDHFQFHRDVSLLRYKKTRNPSMVLFGDES
                     TLFSDHWQKIHRFSFDSSPSVKPVRIL"
     gene            340..1815
                     /locus_tag="PKH10_12020"
     CDS             340..1815
                     /locus_tag="PKH10_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT28888.1"
                     /translation="MIFVPLLLPAAEGNKFDSAQFFRSILEADAVFIGEYLQGGQTKA
                     RIAKKKDLVGSTPADLVVTDIDNQKHWLKYRFAYQPRTPYVFIVNKKGDAYMIHRDSV
                     SIPVMSDRVNFSFSTPYYNNFWQLFNLELLETVILAVREKAAGEIQPATEEKLQGLFK
                     KYIAEKEVNSLKAIIPAAAYLSVRLDETEYAKIVAQADMLGCISVRFSAQVMGDLFFK
                     KQVLAKIRSLPPDAQIAATRSAAATDAKESVRELSELLKSVESYTPPSSECFPEESPE
                     SNKKIFLQAVIELDTPETAKIISRELATEDTAWLSTILSTLADYEGADLVQLALRAAV
                     NERSSERKLEYANYFDRVKSKESAAVFISLFDKNENLFWKKLILSTIGKYGYEESLPF
                     LVKVMQESPNEEVRTTAAMAVGSLDKKDGVLPLYEFIKREKSVLAKSIAVDSMAQIAD
                     KSVQTYLKKIITEEENKKIREQAANAMEDNLFILRYGRKKQ"
     gene            1812..>2177
                     /locus_tag="PKH10_12025"
     CDS             1812..>2177
                     /locus_tag="PKH10_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNT28889.1"
                     /translation="MTDPRQRVAEYLKWQNAVSPFLLPKDLLVKRPSPPAPVAPVEPV
                     ALSSNDSPPTGDGPVTALAALAREIAGCRSCRLGDTRTNLVFGDGDPYARIMFIGEGP
                     GRDEDLQGVPFVGRAGQLLT"
BASE COUNT          489 a          660 c          572 g          456 t
ORIGIN      
        1 aacaggcgaa catgcacaac tggcacccga cgcatcgctg cgcatcgatc actttcagtt
       61 tcatcgggat gtttccctcc tgcgctataa aaaaacaagg aacccgagta tggtgttgtt
      121 cggggacgag tctacgcttt tttccgacca ttggcaaaaa atccatcgtt tttctttcga
      181 ttcttcccct tcggtgaagc ccgtccgaat tttgtaacga taaagtttga caagccgttt
      241 tgtttgtact aatgtaaaaa cgctttgatt tacgatgtga ggtctggaag gaggaccggt
      301 atgtggtttc gccgtttgtg ttcgccagtg ttgttcgcgg tgatcttcgt ccccctgctt
      361 ctgcctgcgg cggagggtaa caagttcgac agcgcgcaat tcttccgttc gatccttgag
      421 gcggacgccg ttttcatcgg cgaatatctc cagggcggcc agaccaaggc gcgtatcgca
      481 aagaaaaaag acctcgtcgg ttcgaccccc gccgatctcg tggtgaccga catagacaat
      541 cagaaacact ggcttaaata ccgtttcgcc taccagcctc gcaccccgta tgttttcatc
      601 gtcaacaaga aaggcgatgc ctacatgatc caccgtgact cggtctcaat ccccgttatg
      661 agcgatcggg tcaatttctc cttcagcacc ccctactaca acaacttctg gcaacttttc
      721 aacctcgaac tgctcgagac cgtcattctc gcggtccgtg aaaaagcggc cggagagatc
      781 cagccggcca ccgaagagaa gctgcaggga ctgttcaaga aatacatagc cgaaaaggag
      841 gtgaactccc tcaaggcgat catccccgcc gccgcctacc tttcggttcg gctcgatgag
      901 acggaatatg cgaagatcgt ggcgcaggcc gacatgctcg ggtgcatatc ggtccgcttc
      961 tcggcgcagg tcatgggcga tctcttcttc aagaaacagg tcctcgcgaa gatacgctcg
     1021 ttgccgcccg acgcccagat agcggccacc cgtagcgccg ccgcgaccga tgccaaggag
     1081 agcgtccgcg aactttcgga actcctgaag tcggtcgagt cctacacgcc gccctcgagc
     1141 gaatgcttcc cggaagaatc tcccgaatcc aataaaaaga tattcctgca ggcggtcatc
     1201 gaacttgaca ctcccgagac cgccaagatc atatcacggg aactcgccac cgaagatacc
     1261 gcgtggctct caacgatact ctcgaccctc gccgactatg aaggggctga tcttgttcag
     1321 ttggcgctcc gcgctgcggt gaacgagcgt tcctccgaac ggaaactgga gtatgccaat
     1381 tatttcgacc gggtgaagtc aaaggaatcg gccgccgttt tcatcagtct gttcgacaag
     1441 aacgagaacc tcttctggaa gaaactcatt ctttccacca tcggtaagta cggctacgag
     1501 gagtcgcttc ccttcctcgt aaaggtcatg caggaatcgc cgaacgagga ggtccgcacc
     1561 accgccgcca tggccgtcgg ttcgcttgac aagaaggacg gcgtgctccc gctttatgag
     1621 ttcatcaagc gcgagaagtc ggtgctcgcc aaatcgatag cggtcgactc gatggcgcag
     1681 atagccgata agagcgtcca gacctacctg aagaagatca tcaccgagga agagaacaaa
     1741 aagatccgcg aacaggcggc gaacgcgatg gaggacaatc tgttcatcct ccgctacggc
     1801 cgcaagaaac agtgaccgat ccccgtcagc gcgtagccga atacctcaaa tggcagaacg
     1861 ccgtcagccc ctttttacta ccgaaggacc tactcgttaa gcgaccctcc cctcccgcgc
     1921 ctgttgcgcc ggtcgaaccc gttgcgcttt cttcgaacga cagccccccg accggcgacg
     1981 gccccgtaac ggcgttggcc gcgctcgccc gggagatcgc cggttgtcgt tcctgtcggc
     2041 tcggagatac ccgcaccaac cttgtcttcg gcgacggcga cccgtacgct cgcatcatgt
     2101 tcatcggcga ggggccgggc cgggatgagg atctgcaggg agtgccgttc gtcggacgcg
     2161 ccggtcaact actcacc
//
LOCUS       DAOQUE010000477         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_63301_length_2176_cov_3.951909, whole genome shotgun sequence.
ACCESSION   DAOQUE010000477 DAOQUE010000000
VERSION     DAOQUE010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63301_length_2176_cov_3.951909"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="PKH10_12030"
     CDS             <1..1371
                     /locus_tag="PKH10_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28890.1"
                     /translation="ALFAIPALLGGAALLGYNHLMTGHLFTFPQDIYFSVIEPIPFCH
                     RIGLGTGCPNTEGEFLPAGGLTLDYAYWVAYTRLTLMLFNVAGHPYPFLFLIVAFFFG
                     LRRNLFLSSFFLTFFIGYFFFYLSGNLFGPRYFTEVTTLLLIPAAAGVVYAARSSPRW
                     STPFIAAFPLAGILFVSTVILPELLSRYSDRFWVTDRSFEKAIEERDIRDSVVFVPAP
                     YHSMFLNLQERPPFDRRGNLILKDLGRENLYGAAFFMETLGLKNAWAIDYYPKLRNLA
                     IVEELPDFRVNDIWIEFEHKGRPATGRPAYATPVAAGVKLTYLPFTTLDEDLNFSKDQ
                     GYAVLFGAPAPDSYYDFTHPILDPGDYELTIELLAAPCGGEGSFTVNDAPVATFSAYD
                     DGYRFKTLRLSAPLRAGTNRFAFIPQAGQSCLILDYIRLKKKDAAPAPEQYPDYTPPE
                     WKELNK"
     gene            complement(1375..>2176)
                     /locus_tag="PKH10_12035"
     CDS             complement(1375..>2176)
                     /locus_tag="PKH10_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28891.1"
                     /translation="IAEEQGATFQPCPEDDADCDKYYNAILFVDSLGRDLYKYDEQSG
                     WMNGLMDPVVPELLVRDNYVSLLGHDPVFIEDMYASERKDAYKIAAPPSGAYALSTAW
                     SERAEMQVYLLRLLYADPDDPEAEFRKTFGTLSDYDTYYASLNEKVTNAFYRHSLHPF
                     NPLFGELFLENLLVVDTLMTQAALDLVIFSPAIPAAARMFEQVTDVVVATDSFTVTYA
                     AGSFEGVAEGTTRTVAWPPYETAGHSVTVSQPDKFVEDVAAWYEKVRR"
BASE COUNT          398 a          743 c          593 g          442 t
ORIGIN      
        1 gccctcttcg cgataccggc gcttctcggc ggcgcggcgc tcctcggcta caaccatctc
       61 atgacggggc acctctttac tttcccgcag gacatctatt tctcggtcat cgagccgatc
      121 ccgttctgtc accgcatcgg gctcggcacc ggctgcccca acaccgaggg cgagttcctc
      181 ccggcgggcg ggctcactct cgattacgcc tactgggtcg cctacacgcg gctcaccctc
      241 atgctgttca atgtggcggg acacccctac ccgttcctct tcctcatcgt cgccttcttt
      301 ttcggactcc gacgcaacct attcctctcc tcgtttttcc ttacattttt cattggctat
      361 ttctttttct acctgtcggg caacctgttc ggcccgcgct atttcactga ggtgacgacg
      421 ctcctgctga taccggccgc cgccggggtc gtgtacgccg cccgctcctc cccgcgttgg
      481 tcgaccccct tcattgcggc gttcccgctc gccggcatac tgttcgtgag cacggtcatc
      541 ctgccagaac tgctgtcgcg ctacagcgac cggttctggg tcaccgaccg ttcgttcgag
      601 aaagcgatcg aggaacggga catccgcgac agcgtcgtgt tcgtccccgc tccctatcac
      661 tcgatgttcc tcaacctgca ggaacgaccc ccgttcgacc ggcgcggcaa ccttattctc
      721 aaggatctcg gccgcgagaa tctttatggc gccgccttct tcatggagac tctcgggctc
      781 aagaacgcgt gggccattga ttactacccc aaactgcgca acctcgccat cgtggaggaa
      841 ctgcccgatt tccgcgtgaa tgatatctgg atagagttcg agcacaaggg gcgtcccgcg
      901 accggcagac cggcctacgc gacgccggtg gcggcgggcg ttaaactgac ctacctcccc
      961 ttcacgacgc tcgacgagga cctcaacttc agcaaggatc agggctacgc ggtgctgttc
     1021 ggcgcacccg ctcccgacag ttactacgac ttcacccacc ccatcctcga cccgggcgat
     1081 tacgaactga ccatcgaact gctcgcggcc ccgtgcggcg gtgaaggatc cttcacggtg
     1141 aacgatgcgc cggtcgcgac cttctcggcc tacgacgacg gctaccgctt caaaacactg
     1201 cggctctccg cgccgctccg ggccgggacc aaccggttcg ccttcatccc gcaggccggt
     1261 cagtcgtgtc tcatactcga ttatatccgg ctcaagaaga aagatgcggc accggcgccg
     1321 gaacagtatc ccgactacac gccgcctgaa tggaaagaac tcaataaata agacctagcg
     1381 gcgcaccttt tcataccacg cggcgacatc ctcgacgaac ttgtcgggct ggctgaccgt
     1441 caccgaatgg cccgccgtct cgtacggagg ccatgccacg gtacgggtcg tcccttccgc
     1501 caccccctcg aacgaaccgg cagcataggt caccgtgaac gaatcggtcg cgacgacgac
     1561 atcggtcacc tgctcgaaca tccgcgccgc cgccgggatg gcaggtgaga agatgacgag
     1621 gtcgagtgcc gcctgcgtca taagggtatc caccaccagc aggttctcga ggaacaattc
     1681 gccgaacagc gggttgaacg ggtgaagcga atgacggtag aaggcattgg tcaccttctc
     1741 gttgagcgac gcgtagtagg tgtcgtagtc ggagagcgtg ccgaatgtct tccggaactc
     1801 cgcctctggg tcgtcgggat cggcgtacag caggcgcagg agatacacct gcatctcggc
     1861 ccgttccgac cacgccgtcg acagggcata cgcaccactc ggcggcgccg ctatcttgta
     1921 ggcatccttc cgttccgatg cgtacatgtc ctcgatgaac accggatcgt gccccaacag
     1981 ggaaacataa ttatcgcgca cgagcagttc gggcaccacc gggtccatga gcccgttcat
     2041 ccaaccgctc tgttcatcgt acttgtagag gtcgcggccg agcgaatcga cgaagagtat
     2101 cgcgttgtaa tacttgtcgc agtcggcgtc atcctcggga cagggctgga aggtcgcacc
     2161 ctgctcctcc gctatc
//
LOCUS       DAOQUE010000478         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_63655_length_2169_cov_6.059603, whole genome shotgun sequence.
ACCESSION   DAOQUE010000478 DAOQUE010000000
VERSION     DAOQUE010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63655_length_2169_cov_6.059603"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PKH10_12040"
     CDS             complement(<1..541)
                     /locus_tag="PKH10_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28892.1"
                     /translation="MTLRAMPFMLLLLVALAPVALFGADRTMPSFTAELFKKQVEAGL
                     GEEEDSRLVRNLTDLYGKFRTLNYPNCFECSLWLVNGIPETPPSKRLILAEYATLFAP
                     DLPESHLNRFLAVLDLRPGDIGLMAHSLGRYLKTTIDTAARDPFIAAVCSIVKLFSSA
                     LFLLFLVVMIGKYGRLLAHL"
     gene            complement(543..1370)
                     /locus_tag="PKH10_12045"
     CDS             complement(543..1370)
                     /locus_tag="PKH10_12045"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNT28893.1"
                     /translation="MPAFMSPDDLHPRLFREDTAIADCRGDLFDLGLGLRQYLEAHIP
                     GAVFIDGEDVLTGPKGTHGGRHPLPEFDQLAQTLGTNGIAQGTTVVAYGMYAARLVFL
                     LNLMGHTEAFVLNGHLDGWIEAGHPTVRKLANRRPLRFTARPDAAMLVEAPELRRLID
                     AGKATLIDVRAPERFRGEVEPIDKVAGHIPGAVNVPWERNFDATGRLLTADALRALYA
                     PHLADTDRVPVLYCGSGVTSCNSLLALREIGVTARLYAGSWSDWISYPDNPVSRGEG"
     gene            1542..>2169
                     /locus_tag="PKH10_12050"
     CDS             1542..>2169
                     /locus_tag="PKH10_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28894.1"
                     /translation="MRSLSLFVGLLAALLFLSCENEVKRSPLDALVTDEAAQNDENTA
                     DDGDTTQNDTTGPTDHEAIPTDGTIPTDEDNVTPDTIGNDDLNDGIMNDDDEIILPDD
                     EPVNVCAMNGGVCIEGIECPLGYTASEQFTCSNAICCMNTGATGLRVDVTGPQPIPAS
                     LISIPSVQISGSTPAVTDDIEAPLLVLGLLEENIDGCKEYKTGATGFRV"
BASE COUNT          437 a          670 c          699 g          363 t
ORIGIN      
        1 acaggtgggc gagcaggcga ccgtatttgc ctatcattac cacgaggaac agcaggaaca
       61 gcgccgagga aaagagtttc acgatcgaac agaccgcagc gatgaaaggg tcccgcgcgg
      121 cggtatcgat cgtcgtcttc agatatctgc cgagagagtg ggccatgagc ccgatatcgc
      181 cgggccgcag gtcgagcacg gcgaggaagc ggttcaggtg ggattcggga agatcggggg
      241 cgaagagcgt cgcgtattcg gcgagtatca gtcgtttcga agggggggtc tcggggatac
      301 cgttcacgag ccacaacgag cattcgaagc agttcggata gttgagcgtg cggaacttgc
      361 cgtacaggtc ggtcaggttg cggacgagtc gcgagtcctc ttcttcgcca agccccgctt
      421 cgacctgttt cttgaaaagt tcggcggtaa aagaaggcat cgtgcgatcg gcgccgaaaa
      481 gggcaacggg cgccagcgcg acgagaagaa ggagcatgaa cggcattgcg cggagcgtca
      541 tatcatccct ctccgcggga taccgggtta tcgggataac tgatccagtc gctccaactg
      601 ccggcgtaga gccgcgccgt caccccgatc tcgcgcaggg cgagcagtga gttgcaggag
      661 gtgacgcccg acccgcagta gagcaccggc acgcggtccg tatcggcaag atgcggggcg
      721 tacagcgccc gcaaagcgtc ggcggtgagc aggcgacccg tcgcgtcgaa gttccgttcc
      781 cacggcacat tgacggcgcc gggtatgtgg cccgcaacct tgtctatcgg ttcgacctcg
      841 ccacggaacc gttccggagc gcggacatcg atgagcgtcg ccttccccgc gtcgatcagg
      901 cgccgcagtt cgggcgcctc gacgagcatc gcggcatcgg ggcgcgccgt gaagcggagc
      961 ggacgccggt tcgcgagctt gcgcacggtc gggtgcccgg cctcgatcca gccgtcgaga
     1021 tggccgttga gcacgaacgc ctccgtatgc cccatcagat tcagcaggaa cacgagccgc
     1081 gccgcgtaca tcccgtaggc gaccaccgtc gttccctgtg ctatgccgtt cgtaccaagc
     1141 gtttgcgcca actgatcgaa ctcggggagc ggatgcctcc ccccgtgcgt tcccttgggg
     1201 ccggtgagca catcttcgcc gtcgatgaag accgcgccgg ggatgtgcgc ttcaaggtat
     1261 tgccgcagac cgagaccgag gtcgaacagg tcgccgcggc agtcggcgat ggcggtgtcc
     1321 tcgcggaaca ggcgggggtg caggtcgtcc ggcgacatga acgcgggcat gaggggcctc
     1381 ttcggttttc gcttcgggaa ctctaccccg cgcacgtcac caaatcaatt ttttcggcga
     1441 tggaccggca aataagggcg ggtatgccgt ccgacttgac aagcggaacg gcgatgctac
     1501 actgccccca ttgaacggca tatcatcgaa ggaggttcgc gatgaggtca ctttccctgt
     1561 tcgtcggcct gctggccgcg cttcttttcc tttcctgcga gaacgaggtc aaacggtcgc
     1621 cgctcgacgc gctcgtgacc gatgaggcgg cgcagaacga cgagaacacc gccgatgacg
     1681 gcgataccac acagaacgac acgaccgggc cgaccgatca cgaggcgata ccgaccgacg
     1741 gcaccatacc gaccgacgag gataatgtca cacccgacac catcggcaac gacgacctga
     1801 acgacggcat catgaacgac gacgacgaga tcatcctgcc cgacgacgaa ccggtcaatg
     1861 tctgcgcgat gaacggcggc gtctgcatcg agggtatcga gtgtcctctc ggctacaccg
     1921 cttctgaaca attcacctgt agcaacgcta tctgctgcat gaacaccggc gcgaccggcc
     1981 tgcgggtcga tgtgaccggc ccccagccga taccggcatc actcatctcg ataccgtcgg
     2041 tccagatatc gggaagcact ccggcggtca ccgacgatat cgaggccccc ttgctggtcc
     2101 tcggcctact ggaggagaac atcgacggtt gcaaggagta taagaccggc gccaccggct
     2161 tccgcgtcg
//
LOCUS       DAOQUE010000479         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_63657_length_2169_cov_4.614948, whole genome shotgun sequence.
ACCESSION   DAOQUE010000479 DAOQUE010000000
VERSION     DAOQUE010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63657_length_2169_cov_4.614948"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PKH10_12055"
     CDS             <1..735
                     /locus_tag="PKH10_12055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT28895.1"
                     /translation="RIFTITESAHRIHNPFTPEKYATLGTALRMEKGTRILDLGSGSG
                     EMLCTWARDHGITGTGVDLSPLFTEQAKRRAEELGVADKVKFIHGDAAGYVSAEKVDV
                     AACIGATWIAGGVAGTIELLARSLRPGGIILIGEPYWRQMPPTEEVAQGCEGVAIADF
                     LPLPELIASFGRLGYDVVEMVLADQEGWDRYEAAKWLTMRRWLETNPHDEMAKEVRAR
                     LTEDPERYAKYTREYLGWGVFALMKR"
     gene            805..1386
                     /locus_tag="PKH10_12060"
     CDS             805..1386
                     /locus_tag="PKH10_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNT28896.1"
                     /translation="MKRLSLFIALVAAFVLSSCGYNTMQTQEEEVFKAWGDLESTLQR
                     RADLIPNLVSTVKGYAAHEAETLEKIVAMRAQVGQTKIEAKDLGSADTMAKFQKAQGE
                     LSAGLGRLLVVAEQYPDLKANENFRDLQSQLEGTENRITVARNRYNEAVSTFNASIRG
                     FPNNIINGWLLHLEKKEYFKADEKAKEVPKVQF"
     gene            1390..2094
                     /locus_tag="PKH10_12065"
     CDS             1390..2094
                     /locus_tag="PKH10_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HNT28897.1"
                     /translation="MRRLIFLIPFFCASLFALAVPPLTGRITDRAELLSPDAKARIEA
                     KLAALETSDSTQIAVLTVPTLDGDSIEDFSMRVVESWELGQKKLDNGALLVIAKDDRK
                     LRIEVGYGLEGKLTDALSSRIIRRYIVPRFKKNDFAGGVEAGVDAMIAAVKGEFTAGE
                     EISAKIEQFVPLVFLLLLILVFVFGSRRSSSRRSRHYGGGGFFGGGFGGGGGFGGGGF
                     SGGGGGFGGGGSSGGW"
BASE COUNT          435 a          655 c          701 g          378 t
ORIGIN      
        1 cggatattca ccatcaccga aagcgcccac cgcatccata accccttcac ccccgaaaaa
       61 tacgccaccc tcggcaccgc cctgcgcatg gaaaagggga cccgcatcct cgacctcggc
      121 agcggttcgg gcgagatgct gtgcacatgg gcgcgcgacc acggcatcac cggcaccggc
      181 gtcgacctga gcccgctctt caccgagcaa gcgaaacgcc gcgccgaaga actcggggtc
      241 gccgataaag taaaattcat ccacggcgac gcggcgggct atgtctccgc cgagaaggtc
      301 gatgtggcgg cctgtatcgg cgccacctgg atcgctggcg gtgtcgccgg cactatcgag
      361 cttctggctc ggagcctccg ccccgggggg atcatcctca tcggcgagcc ctactggcgg
      421 cagatgccgc cgaccgaaga ggtcgcccaa gggtgcgaag gtgtcgccat cgccgatttt
      481 ctcccgctcc cggaacttat cgcctcgttc ggccgcctcg gctacgatgt cgtggagatg
      541 gtcttggcag atcaggaggg ctgggaccgg tacgaagcgg ccaagtggct caccatgcgc
      601 cgctggctgg agaccaatcc ccacgacgag atggcgaaag aggtccgcgc ccgactgacc
      661 gaagacccgg agcgctacgc caaatacacc cgtgaatatc tcggctgggg cgtgttcgcg
      721 ctgatgaagc ggtgatcgtc gccgaagttg actcctgagg cgtttgtcgc tacagtaccc
      781 ctgtcttttt tcatggagga gacgatgaag agactgtcgc tgttcatagc gctcgtcgcg
      841 gcgttcgtat tgagttcctg cggctacaac accatgcaga cacaggaaga ggaggtcttc
      901 aaggcgtggg gtgatctcga atcgaccctc cagcggcgcg ccgacctcat ccccaatctg
      961 gtcagcaccg tcaagggcta cgcggctcac gaagccgaga cgcttgaaaa gatcgtggcg
     1021 atgcgggcgc aggtcggcca gacgaagatc gaggccaaag acctcggtag cgccgatacg
     1081 atggcgaaat tccagaaggc gcagggggaa ctgtcggcgg gactcggtcg attgctggtc
     1141 gttgccgaac agtaccccga cctcaaggcg aacgagaact tccgcgacct acaaagccag
     1201 ttggagggga ccgagaaccg gataacggtc gcgcgcaacc gctacaacga ggcggtgagc
     1261 accttcaacg catccattcg cggcttcccg aacaacatca tcaacggctg gcttctccat
     1321 cttgagaaga aggagtactt caaggccgac gagaaggcga aggaagtgcc caaggtgcag
     1381 ttctagccga tgcggcggct catattcctc ataccgttct tctgcgcgtc gctcttcgcg
     1441 ctcgcggtac cgccgctcac cgggcggatc accgaccggg cggagttgtt gtctcccgac
     1501 gccaaagcac ggatagaggc gaaactggcg gcgcttgaaa cgagcgattc gacccagatc
     1561 gcggtgctca ccgtgccgac actcgacggc gacagtatcg aggacttctc tatgcgggtg
     1621 gtcgagagtt gggaactggg gcagaagaag ctcgacaacg gtgcgctgct ggtcatagcg
     1681 aaggatgacc gcaaactgcg catcgaggtc ggctacgggc tggaagggaa actgaccgac
     1741 gccctgtcga gccgtatcat ccgccgctac atcgtcccgc gcttcaaaaa gaacgacttc
     1801 gcaggcggtg tcgaggcggg cgtcgatgcg atgatcgccg cggtgaaagg ggagttcacg
     1861 gccggggagg agatatcggc gaagatcgaa cagttcgtgc cgctcgtgtt cctcctcctg
     1921 ctcatactgg tattcgtctt cggttcacgt cggagcagtt cccgccggtc ccgccattac
     1981 ggcggcggcg gtttcttcgg cggcggcttc ggcggcggtg gtggtttcgg cggcggtgga
     2041 ttttcagggg gcggcggcgg cttcggcggc ggcggatcct cgggagggtg gtgatatgcg
     2101 cgatctggcg gaacgattcc tgagccccca cgaaaaagag agcatcatcc gctcggtgaa
     2161 agaggttga
//
LOCUS       DAOQUE010000480         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_64161_length_2158_cov_3.936282, whole genome shotgun sequence.
ACCESSION   DAOQUE010000480 DAOQUE010000000
VERSION     DAOQUE010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64161_length_2158_cov_3.936282"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PKH10_12070"
     CDS             complement(<1..462)
                     /locus_tag="PKH10_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28898.1"
                     /translation="MRRFFVFIAVFALVAVGTCAKKESDIEKTSKKVAAASGGTVSLD
                     SGDAFIQIPAGALDTDVEITIVKKKRDGYPQAEACVSYVYEFGPSGLTFKVKATMGIK
                     PHEGYIPADGKVPTGNALVAATLRDGSWHVGDAAAGDPIMAGDPIMAGDPIM"
     gene            623..>2158
                     /locus_tag="PKH10_12075"
     CDS             623..>2158
                     /locus_tag="PKH10_12075"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HNT28899.1"
                     /translation="MRCGLVPALCLLSLLLVACSSSAPADTDDQTAESDASSNDGDHL
                     LIPDDDAATGNPLFDKGPYGVAIGDTAANFTVPTDTGDWDLEEHWDGKDHQVLLFYRS
                     ANNYAKQMFQSRLVMLLMNSPKNVHYFFIAGGVGEAVAEMLDPVRAEAESALDIIDDP
                     GHWRPRLHFVTKGASEIGGWLSEWYAAGGGFILGVDRAQKIRGAGLPLNWLTQEGDFE
                     MAGYEPAYWEFDLKRAEALGAEADPIVLTGIDGQEFESETITFEVEFPAAAAMADHTG
                     LAVDLEQVCASQAACEWDRIMHLYICGPDDGDTCGTEVARWITAYGMGGRWVTDIKPL
                     LPLFASGGKLKFRLYVWGYQAKNYLSFRLTKGGDPAPVGWKKLFVGNPRFDENYNTQY
                     EPVAFELPTGAQKAAIVAFITGHGNGSEQANCAEFCPFESVFVVNGTPFEKDHPDAAT
                     NDGCLLQVGDGVVPNQYGSWPFGRAGWCPGLNVSPWVAEVTDALVSGENTVDTSASVT
                     SATH"
BASE COUNT          395 a          650 c          673 g          440 t
ORIGIN      
        1 catgatggga tcgcccgcca tgatgggatc gcccgccatg atgggatcgc ccgccgccgc
       61 atcgccgaca tgccacgagc cgtcacgcag ggtagcggct accagagcgt tgcctgtcgg
      121 gaccttcccg tctgcgggga tgtatccttc gtgtggcttg atacccatgg tcgccttgac
      181 cttgaaggta agcccgctcg ggccgaactc gtagacataa ctgacgcatg cttcggcctg
      241 tgggtagccg tcgcgtttct ttttcacgat cgtgatctcg acatccgtat cgagggcgcc
      301 ggccggtatc tggatgaagg cgtcgcccga atccagtgac acggtgccgc ccgacgcggc
      361 ggcgaccttc ttgctcgtct tctcgatgtc gctctccttc ttggcgcagg tgccgaccgc
      421 gacgagcgcg aaaacggcga taaaaacgaa aaatcttctc atgggaccct ccgttcggtg
      481 catgatgacc tccaaaccac cgcgccgatt atagaaaaag ggaagcggaa aagcaatgtt
      541 tcgatatctt ccggatattt tttgcgtttg tcccgatttt cgattatttt caaggggtcg
      601 ttacccacag ggagatgttg ttatgagatg tggccttgtt cccgcgcttt gccttctttc
      661 tctcctgctg gtcgcctgtt cctcgtcggc accggcagat accgatgacc agacggccga
      721 gagcgacgca tcatcgaacg acggcgatca cctgcttatc ccggatgatg acgccgcgac
      781 ggggaacccc ctcttcgaca agggaccgta cggcgtcgcc atcggcgata ccgccgcgaa
      841 cttcaccgtg ccgaccgaca cgggcgactg ggatcttgag gagcattggg acggaaagga
      901 tcatcaggtc ctgctcttct accgttcggc gaacaactat gctaaacaga tgttccagtc
      961 gcggcttgtg atgcttctca tgaactcgcc gaagaatgtc cactattttt tcatcgccgg
     1021 tggcgtgggt gaggcggtgg ccgagatgct cgacccggtc cgcgccgagg cggaatcggc
     1081 gctcgatatc atcgatgacc cgggtcactg gcgcccgcgg ctccatttcg tgacgaaggg
     1141 ggcgtcggag ataggcggtt ggctgtcgga atggtacgcc gccgggggcg gattcattct
     1201 cggtgtcgac cgcgcccaga agatacgggg tgccggcctg ccgcttaact ggctcacgca
     1261 ggaaggtgac ttcgagatgg cgggctacga acccgcgtac tgggaattcg acctcaaacg
     1321 agcggaagcc ctcggtgccg aggcagatcc gatcgtcctc accggcatcg acgggcagga
     1381 gttcgagagc gagacgataa ccttcgaagt ggagttcccc gccgctgccg caatggccga
     1441 ccacaccgga ctcgcggtcg atctcgaaca ggtctgtgcc tcgcaggcgg cctgcgagtg
     1501 ggaccgcatc atgcacctct acatctgcgg tcccgacgat ggcgatacct gcggcaccga
     1561 ggtggcgcgc tggataacgg cctacgggat gggcggccgc tgggtgaccg acatcaagcc
     1621 gctcctgccg ctgttcgctt cgggcgggaa actcaagttc cggctctatg tctggggcta
     1681 tcaggcgaag aactatcttt cgttccgcct gacgaagggc ggtgacccgg cgccggtcgg
     1741 ttggaaaaaa ctctttgtcg gcaacccgcg gttcgacgag aactacaata cccaatacga
     1801 gccggtggcg ttcgaactgc cgaccggggc gcagaaggcg gccatcgtcg ccttcatcac
     1861 cggccatggc aatggttcgg agcaggcgaa ctgcgccgag ttctgtccct tcgagtcggt
     1921 cttcgtcgtc aacggcactc cgttcgagaa agatcatccc gacgccgcga ccaatgacgg
     1981 ctgtctgttg caggtcgggg atggtgtcgt cccgaatcag tacggcagtt ggccgttcgg
     2041 tcgcgccggc tggtgtcccg gtctcaatgt gtcgccgtgg gtcgccgaag tgaccgatgc
     2101 actcgtgtcg ggggagaata ccgtcgacac gagtgcatcg gtcacttcgg cgacccac
//
LOCUS       DAOQUE010000481         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_64452_length_2152_cov_4.003815, whole genome shotgun sequence.
ACCESSION   DAOQUE010000481 DAOQUE010000000
VERSION     DAOQUE010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64452_length_2152_cov_4.003815"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKH10_12080"
     CDS             complement(<1..315)
                     /locus_tag="PKH10_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28900.1"
                     /translation="MTAIERLIETLKPRHPDLSRFTVVSLGGSGRDIIHYTLHKQLGG
                     LMPAVLEFDEYKSRRIAEATGHRPLPDDEAFCRFHALHRSMKKEDPPGPADTERLMQF
                     LSL"
     gene            474..1658
                     /locus_tag="PKH10_12085"
     CDS             474..1658
                     /locus_tag="PKH10_12085"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNT28901.1"
                     /translation="MTSKTFFILFLALFVLACDDNKQQLADLEPVNDETQTDTDTAGN
                     DTVPTDGSDALLPDEDGLIPTDDDVVTDTPTDADTVVVVTCAGLKDGENTILVNGEES
                     KDLARTFLLRLPTTIEDKKGWPVIFNWHGYGDTAANFEWLLSGSVNNTELPFILVTPE
                     DTNLAINQYPPAGVDWDILTLSNGSIEAELFDAVLACIEERWGVDEDRIHLAGFSAGS
                     ITADSLGVLRGDLIASIFTYSGAYFSDAKAKADLGTMAAGFISWPTMETDNKYVQAFM
                     YGAEGDADCTGPTLCDKWGSSGFYINFNHMARFNANYLTGMGHTVILCDHDKGHTNAG
                     LSSTQLLKFFLDHPRGTVTSPYATDWPADYPDYCFLMTEPDPTIEPGYETPDDDSLLP
                     DA"
     gene            1666..>2152
                     /locus_tag="PKH10_12090"
     CDS             1666..>2152
                     /locus_tag="PKH10_12090"
                     /inference="COORDINATES: protein motif:HMM:NF014719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNT28902.1"
                     /translation="MRTFFSVIAGLAFFLYGIDLLSALLGRMAGGRLQERLDRLASTD
                     GQRYWYGALTTVLFQSSSATTVLLVSMASAGLVTATEAFSFTLGANLGAVSTGWIAAV
                     HITVIGLPLFIIGVVGRFFSTRAARRDLFSFCIGLGLILFGLDLMGQGMEAIHESGDV
                     TA"
BASE COUNT          410 a          693 c          648 g          401 t
ORIGIN      
        1 gagcgaaagg aactgcatca gccgctcggt gtcggcggga cccggcgggt cctctttttt
       61 catggaccgg tgcagggcat ggaaacggca gaaggcttcg tcatcgggga gcggccggtg
      121 accggtcgcc tcggcgatgc ggcgactctt gtactcatcg aattcgagga cggcgggcat
      181 aagtccgccg agttgtttgt ggagggtgta gtggatgata tcgcggcccg aaccgccgag
      241 cgacacgacg gtgaagcggg acaggtcggg gtggcggggt ttaagcgttt cgatgagtcg
      301 ttcgatcgcg gtcattcctc tccctcggat cggccacgcc attgtagcgt gccgttcaga
      361 ttttacaaga taacggggga cgcgggatgt ccccgacggc aagatttgac aacacgcggg
      421 ggaacgctat cctatagcga gcgacaccga tccaccacaa agggagcacg gccatgacga
      481 gtaagacctt tttcatcctt tttctcgcgt tgttcgtcct cgcctgtgac gacaacaagc
      541 aacaactcgc cgacctcgag ccggtgaatg acgagacgca gaccgacacc gacacggctg
      601 gtaatgacac ggtccccacc gacgggagcg acgcactcct gcccgacgag gatggactca
      661 tcccgaccga tgacgatgtc gtcaccgaca cgccgaccga cgccgacacc gtggtcgtcg
      721 tcacctgcgc cggactcaag gacggcgaga acacgatact cgtgaacggc gaggagtcga
      781 aggatctcgc gcggaccttc ctgctccgcc tgccgacgac gatagaagac aagaaggggt
      841 ggccggtgat attcaactgg cacggctacg gcgacaccgc cgcgaatttc gaatggcttc
      901 tttccggttc cgtgaacaac accgagttac cgttcatcct cgtcactccg gaagacacca
      961 acctcgcgat aaaccaatac ccgcccgccg gggtcgattg ggacatcctg acgctgagta
     1021 acggctccat cgaggcggaa ctgttcgacg cggtgcttgc ctgcatcgag gagcggtggg
     1081 gcgtcgacga ggaccgtatc cacctcgccg ggttctcggc cggctcgata acggccgaca
     1141 gcctcggcgt cctccgcggc gatcttatcg cctcgatatt cacctattcc ggcgcctact
     1201 tcagtgacgc gaaggcgaag gccgacctcg gcacgatggc ggccggcttc atatcgtggc
     1261 ccacgatgga gaccgacaac aaatatgtcc aggccttcat gtacggcgcc gagggtgacg
     1321 cggactgcac aggacccacg ctctgcgaca agtggggctc ttccggcttt tacatcaact
     1381 tcaaccacat ggcccgcttc aacgccaatt acctgaccgg catggggcac accgtcatac
     1441 tttgcgacca tgacaagggg cacaccaacg cgggactcag ttctacgcag ttgctgaagt
     1501 tcttcctcga ccatccgcgc ggcaccgtca catcacccta cgcgaccgac tggcccgccg
     1561 attaccccga ctactgcttc ctcatgaccg agcccgaccc gacgatagag ccgggctacg
     1621 agacgcccga cgacgattcg ctcctgcccg acgcctgaag ccggtatgcg caccttcttc
     1681 tcggtcatcg ccgggctcgc attcttcctg tacggcatag acctgttgag cgccctgctc
     1741 ggtcgcatgg cgggcggacg actgcaggaa cggctggacc ggctcgcctc gaccgacggt
     1801 cagcgttact ggtacggcgc gctgacgacg gtgctgttcc aatcctcgag cgcgaccacc
     1861 gtccttcttg tcagcatggc ctccgccggg ctcgtcacgg cgaccgaggc cttttcgttc
     1921 acgctcggcg ccaatctggg cgcggtatcg accggctgga tagcggcggt acatatcacc
     1981 gtcatcggcc tgccgctctt catcatcggc gtggtcggtc ggttcttttc gacccgcgcg
     2041 gcgcggcgcg acctgttctc tttctgcatc ggactcgggc tcatcctgtt cggactcgac
     2101 ctgatgggac agggcatgga ggcgatacac gaaagcggcg atgtgaccgc ct
//
LOCUS       DAOQUE010000482         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_64539_length_2150_cov_4.770883, whole genome shotgun sequence.
ACCESSION   DAOQUE010000482 DAOQUE010000000
VERSION     DAOQUE010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64539_length_2150_cov_4.770883"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="PKH10_12095"
     CDS             complement(<1..1469)
                     /locus_tag="PKH10_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HNT28903.1"
                     /translation="MSRSAGILFLAVLCFLSVLSAAEPCPAGWTFNSANNHCYNLEAT
                     ASPVTWVQARAACAAYNARTTLVTPGSQAENDYVYNYVKTEKAYNGNIWIGLEGNDAY
                     AASGNETCSAALIAPSMGRKYSGSIGTGTGTVDPYGYCDMPGKENFFRFTAGVGGTWT
                     FKVYSTFDAVIDLYSGACTSYIDCEDYYGANATDTMTYTLTAGQTYTVVVDKYRTTDS
                     GTYTLEIMAPTDNGKRAYSWIDNTDTTYTNWDSGEPNNSGGAEACAELKIGTTTYGKW
                     NDVPCDSSGALHAYVCEYVSSICGDTVVEGIEECDDGNLTDGDGCDSNCRPTGCGNGL
                     VTAGEQCDDGNVIDLDGCKNDCTLPCPDGWVYSTATGNCYNLYGVGDTKTWIEADAAC
                     ETAYGNGTTLATIGDATENAFIFNYVKNTKAYNGNIWLGMEGETAYPSFGNETCSTAW
                     MAPSMGRKYSGSIGTGTGTVDPYGWCDMPGKENFFRFIAG"
BASE COUNT          394 a          603 c          616 g          537 t
ORIGIN      
        1 ccggcgatga accggaagaa gttctctttt cccggcatgt cgcaccagcc gtacgggtcc
       61 accgtgccgg tcccggtccc gatggagccg ctgtatttgc ggcccatgga gggggccatc
      121 catgcggtgg aacaggtctc gttgccgaaa gagggatagg cggtctctcc ttccatgccg
      181 agccagatat tgccgttgta ggccttggtg ttcttgacat agttgaatat gaaggcgttc
      241 tcggtcgcgt cgccgatggt ggccagcgtg gtgccgttgc cgtaggcggt ctcgcacgcc
      301 gcgtcggcct ctatccatgt cttggtgtca ccgacgccgt agagattgta gcagtttccg
      361 gtcgcggtgc tgtagaccca gccgtcggga cacggaaggg tacaatcgtt cttgcagccg
      421 tcgaggtcta tcacattgcc gtcatcgcac tgttcgccgg cggtcaccag accgttgccg
      481 cacccggtcg ggcggcagtt gctgtcacag ccgtcgccgt cggtgaggtt gccgtcgtcg
      541 cattcctcga taccttcgac gaccgtgtcg ccgcatatcg acgatacata ttcacagaca
      601 taggcatgaa gcgcgccgct gctgtcgcag gggacatcgt tccacttccc gtaggtcgtc
      661 gtaccgatct tgagttccgc gcaggcctcg gcgccgccgc tgttgttcgg ttcgccgctg
      721 tcccaattgg tgtaggtcgt gtcggtattg tctatccatg aataggcccg cttcccgttg
      781 tcggtcggcg ccatgatctc gagcgtgtag gtccccgaat cggtggtgcg atatttgtcc
      841 acgacaacgg tataggtctg gcccgctgtc agcgtgtagg tcatcgtgtc ggtcgcgttc
      901 gcgccgtagt agtcctcgca gtcgatgtag gaggtacagg cgccgctgta gagatctatc
      961 accgcgtcga aggtcgaata aaccttgaag gtccaagtcc cgccaactcc ggcggtgaac
     1021 cggaagaaat tctcttttcc cggcatgtcg cagtagccgt aaggatcgac cgtaccggtc
     1081 ccggtcccga tggaaccgct gtatttgcgc cccatcgacg gagcgatgag ggcggctgaa
     1141 caggtctcgt tgccgctcgc ggcgtaggcg tcgtttcctt cgaggcctat ccagatattg
     1201 ccgttatagg ccttttcggt ctttacatag ttatagacat agtcgttctc cgcctgcgaa
     1261 ccgggcgtca cgagcgtcgt ccgcgcgttg taggcggcgc aggccgctct cgcctgtacc
     1321 caagtgaccg gcgacgcggt cgcttcgaga ttgtagcagt ggttgttggc gctgttgaag
     1381 gtccagcccg ccggacacgg ttcggcggcc gaaaggaccg acaggaaaca gagcacggca
     1441 aggaacagga taccggctga acgactcatg acttcctccc ataaagtata tctattgatc
     1501 gaacgatcat cgtggtgccg ggaaggagaa ccgaccgttc tcccgtgtcc cccgtggata
     1561 tgaaagacag tataccgcac caagctatac cccgatagcc ggaacttttc tatgtctttt
     1621 atgggtggat taaatcacgg cgaaaaccta atttcaactt ttgttaccgg atcgttgttt
     1681 ttttgagtgg tcactccgaa gtgtactctc tgacccgtaa cttcttgccg attatgaatc
     1741 tttctttttt aggactcgaa agttcttttc cttttgcaca aaacattttt gtgaaaatat
     1801 cttcttgtat tatcgaacag ttcgtcgatt tttcgggctc tttctttaaa agttatcgtc
     1861 ctgaatgatt tcaagaagat agggtcagcg catgtgtttt atgctgattc taacagggcg
     1921 ttcctcttaa tcttggtcgg aaaaagtctg ggaagcgggc cgaggccccg ggatcggggg
     1981 tgtcaacggg aggtctgggc gagaaggatc agtaactaca acatattcac cgtaccaaat
     2041 ctccgtccct tccgccgtcg cgctgctttg gcgggacaag ccccttctcc ggcgggcgcc
     2101 ggtccttctc cacttcctcg atcgcgctct tctgcgcgag cgacttcttc
//
LOCUS       DAOQUE010000483         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_64640_length_2148_cov_3.123746, whole genome shotgun sequence.
ACCESSION   DAOQUE010000483 DAOQUE010000000
VERSION     DAOQUE010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64640_length_2148_cov_3.123746"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="gspN"
                     /locus_tag="PKH10_12100"
     CDS             <1..1176
                     /gene="gspN"
                     /locus_tag="PKH10_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HNT28904.1"
                     /translation="MKERILSILRDLWPLLRTVGDRLFSGGRLAAAARGALFIGVASM
                     IGWYLALALILPKDLVLGRIDQAASGFDLKIEAEDVGYSVFIAATLKGGSVVGASGET
                     LMTFDRLTVSPDLFSLLTDTPSATVTIEDIDGKGGELTVAFGTGEEACYRLEAEEVSL
                     SIVRLLWPDVRMGGIMNGQVDLCKGKKMSGELLLAMREALLGGKVYGLELEKDIELGA
                     IDIDASIKDNKLDIRKMQSDGAVEIEVTGKVTMNPSNYKNSRLDLNATLTEKKEGFLK
                     SLPIIELALSRFGTGGGYAVKMTGPLSSPSVRQGSRSKTPRIAKPSRPDFAGESTVKK
                     GKERERDRMKERTEEKKEPPPEEEKKGPEPENKEPENKEPEKKEPEKEEKPDMEEKP"
     gene            1173..1685
                     /locus_tag="PKH10_12105"
     CDS             1173..1685
                     /locus_tag="PKH10_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28905.1"
                     /translation="MKATLLCLLASGSLLVSCATANIQGTDIPDTPQNRAVIQVFFEY
                     VEAVKAQNADKLLALVSEHYLDANGTDEATDDVDYEGVKKFVNSDAYRNIRAVSTFFI
                     VKDMSVSPDEQTAKILYYYEVRIKTDRLAPPEEGSSINSDTQKWLKETEVNQMLLKKE
                     GDKWKIVSGL"
     gene            complement(1689..1928)
                     /locus_tag="PKH10_12110"
     CDS             complement(1689..1928)
                     /locus_tag="PKH10_12110"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28906.1"
                     /translation="MDAFTSSPRTAHCTFSQKVTNAANAGALALIIYNNVADNNWGIQ
                     ADGPIPVAGISQASGDEIITFTGEHPEDIAVAIRP"
BASE COUNT          486 a          571 c          717 g          374 t
ORIGIN      
        1 atgaaggaac ggatactatc gatcctgcgg gacctgtggc ccctgttgcg aacggtcggg
       61 gaccggttgt tcagcggtgg tcggctcgcc gcggctgctc ggggcgcgct gttcatcggt
      121 gtcgcttcga tgatcggctg gtatcttgcg ctcgcgctga tcctccctaa ggacctggtg
      181 ctcggcagga tcgatcaggc ggcatcgggg tttgacctga agatagaggc ggaggatgtc
      241 ggctattcag tcttcatcgc cgccaccctg aaaggcggtt cggtggttgg agcgagcggc
      301 gaaacgctca tgaccttcga ccggcttacc gtgtcccccg atctcttctc gttgttgacc
      361 gatacgccgt cggcgaccgt gacgatagag gacatcgacg gcaagggcgg tgaattgacg
      421 gtagccttcg gtaccggcga agaggcctgt taccggctgg aggccgagga ggtgtcgctc
      481 tcgatagtgc gtctgctgtg gcccgatgtc cgcatgggcg gcatcatgaa cgggcaggtc
      541 gatctctgca aggggaaaaa gatgtcgggc gagttgttgc tcgcgatgcg ggaggcgctg
      601 ctcggcggga aggtctacgg gctggaactt gaaaaagaca tagaactggg tgccatcgat
      661 atcgacgcga gcatcaagga caacaagttg gatatccgca agatgcagag cgatggcgcg
      721 gtggagatag aggtgaccgg taaggtgacg atgaacccgt cgaactacaa gaactcgcgg
      781 ttggacctca acgcgaccct gaccgagaag aaggaggggt tcctgaaaag cctgccgatc
      841 atcgaattgg ccctgtcccg tttcgggacc gggggcggat atgcggtcaa gatgaccggg
      901 ccgctttcga gtccgagcgt ccggcaggga agccgttcga agaccccgcg tatcgccaaa
      961 ccgtcccggc ccgatttcgc cggggagagc acggtcaaga aaggaaagga aagagaacgg
     1021 gaccggatga aggaacggac ggaggaaaag aaagagccgc cgccggaaga ggagaaaaaa
     1081 ggaccggagc cggagaacaa agagccggag aacaaagagc cggaaaagaa agaaccggaa
     1141 aaagaagaaa aacctgatat ggaggaaaaa ccatgaaagc gaccctgttg tgtctgctgg
     1201 cgagcggaag cctgctcgtt tcctgtgcca ccgcgaacat tcaagggacc gacatccccg
     1261 ataccccgca gaaccgggcg gtgatacagg tgttcttcga gtatgtcgag gcggtcaagg
     1321 cgcagaacgc cgacaaactg ctcgcgctcg tttccgaaca ctacctcgat gccaacggca
     1381 ccgacgaggc gaccgacgat gtggattacg agggggtcaa gaaattcgtc aattcagacg
     1441 cctaccggaa catccgcgcg gtgagcacct ttttcatcgt caaggacatg agcgtgtcgc
     1501 ccgacgagca gacggcgaag atactctact actacgaggt gcgcatcaag accgaccgtc
     1561 tggcgccgcc cgaagagggc tcttcgataa actccgatac gcagaagtgg ctgaaggaga
     1621 ccgaggtcaa ccagatgctc ctcaaaaagg aaggcgacaa atggaagatc gtttcggggc
     1681 tgtgaccctt acggtctgat cgcgaccgcg atgtcctcgg ggtgttcgcc ggtgaaggtg
     1741 atgatctcgt cgcccgacgc ctgcgagatg cccgctaccg gtatgggccc gtcggcttgt
     1801 atcccccagt tattgtcggc cacattgttg tagatgatga gggcgagcgc gccggcgttc
     1861 gccgcgttgg tgaccttctg cgagaaggtg cagtgggccg tgcgcgggct gctagtgaag
     1921 gcgtccaatg cggccgcaag cgctcagttc acgagcagcg gagggtccaa tgcaggcgat
     1981 tcacacgctc aaccaagcgc tgggagcgga agtgcacctt ctcgcagaag ggtgcgggac
     2041 acttggagcg tagcatgggt gcgagttcgg tcaagggcag aatgcttggc gccattcagc
     2101 tgacaagcgc actcgcaccg tgaacccgct cgtcgcctac tcggaacg
//
LOCUS       DAOQUE010000484         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_64667_length_2147_cov_5.786807, whole genome shotgun sequence.
ACCESSION   DAOQUE010000484 DAOQUE010000000
VERSION     DAOQUE010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64667_length_2147_cov_5.786807"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKH10_12115"
     CDS             complement(<1..331)
                     /locus_tag="PKH10_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28907.1"
                     /translation="MKKTACIAFLLLTLTASSASAASLFETVGSPDLMHPFNLRVIAL
                     GPEAAYYNPALLIGQKDQLKLGFGMSAGDFNIAYMRKDSKYDIPGEIRDRNSSLITHD
                     GAEVPLED"
     gene            complement(342..809)
                     /locus_tag="PKH10_12120"
     CDS             complement(342..809)
                     /locus_tag="PKH10_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNT28908.1"
                     /translation="MLRTMLCALTLLCGAVLTAGELDSPVGYWMTIDDNDGKPRGIVK
                     IEEVNGELVGHIVKVFPRPGEEDNPVCVKCKGEMAGKRAIGMRVLWGMKKDGDEWNGG
                     GVMDPDNGETYKGYLKLTFFGSKLKLRGYVGISLFGRSQEWIRQQNFKETTNK"
     gene            complement(812..>2147)
                     /locus_tag="PKH10_12125"
     CDS             complement(812..>2147)
                     /locus_tag="PKH10_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT28909.1"
                     /translation="LRGEPEVTEMTTVIGDDLPKFYVTMFPQPPSKEYGQAMFRVDLE
                     KGGRFADNERFGYHLQQKLDATVTGGSAKVKILQQAEPMDAPVMFRLTGKDLRRLLTV
                     SRTLQDELRTVPGLMNVRDNAASERFEYTVKVDEEVATSLGIVKYDVQRQINLALRGD
                     KASVYRTGGNEFDIIVESDIDSVEELGNLAIKSTIAGNKVLLKQIAEITLTSKMDYIY
                     RFDKEHSVSVLADVAPGYGAPEITARFMHEALPRLDTAGVRVVHDGEMKNIIDNFTNL
                     AIAALAALLFNYLILLLEFKSFLQPLIILTTVPLSVIGALMGLLIFGQPFSFTAFLGI
                     TSLIGVVVNNAILLMEYINDEKGKGHDLKKACVEAVARRYRPIMLSNVTTVMGLVPLA
                     TSGSQLFGPMSIAQMSGLLVATLLTLVVIPIVYYRAVTTMNAYREQWKGGAS"
BASE COUNT          389 a          651 c          646 g          461 t
ORIGIN      
        1 tatcctcaag cggcacctcg gcgccgtcgt gggtgatgag actcgagtta cgatcgcgta
       61 tctcaccggg gatatcgtac ttcgaatcct tccgcatata ggcaatgttg aagtcgcccg
      121 ccgacatgcc gaagccaagc ttcaattgat ctttctgccc gatgagaaga gccggattgt
      181 agtaggccgc ttcaggaccg agcgcgatga cccgcaggtt gaacgggtgc atgaggtcgg
      241 gcgacccgac cgtttcaaac agcgatgcgg cgctcgcgga tgatgccgta agcgtcaaca
      301 gcaggaaggc gatacaggcg gttttcttca tcgtcgttct cctatttgtt ggtcgtttcc
      361 ttgaaattct gttggcgtat ccattcctgc gagcgaccga acagtgagat acctacatag
      421 ccgcgcaact tcagtttgct accgaagaaa gtgagtttca ggtaaccctt gtaggtctcg
      481 ccgttgtcgg ggtccatcac cccgccgccg ttccactcgt cgccgtcctt cttcatcccc
      541 cacaggacgc gcatcccgat ggccctcttt cccgccatct cccctttgca tttgacacag
      601 accgggttgt cctcctcgcc gggacgagga aagaccttga cgatgtggcc gaccagttcg
      661 ccgttcacct cttcgatctt gacgatgccg cgcggcttgc cgtcgttgtc gtcgatggtc
      721 atccagtagc cgacggggct gtcgagttcg cccgccgtca ggaccgcgcc gcacaggagc
      781 gtcagcgcgc aaagcatcgt acgcagcatc ttcacgaggc acctcctttc cattgttccc
      841 ggtatgcgtt catcgtcgtc acggcgcggt aatagacgat ggggatgacc acgagcgtca
      901 gcagcgtcgc gacgagcagt ccgctcatct gggcgatcga catcggcccg aacagttggc
      961 tgcccgaagt ggcgagcggg accagcccca tcacggtggt cacgttcgac agcatgatgg
     1021 gacgatagcg acgagcgacc gcctcgacgc aggccttttt gagatcgtgt cctttccctt
     1081 tctcgtcgtt gatgtattcc atgagcagga tggcgttgtt gaccaccacc ccgatgaggc
     1141 tcgtgatgcc gaggaatgcg gtgaacgaga agggctggcc gaaaatcagc aggcccatca
     1201 gcgccccgat gaccgatagc ggcaccgtgg tcaggatgat gagcggctgg aggaacgact
     1261 tgaattcgag caggaggatc aggtagttga acagcaatgc cgcgagcgcc gcgatggcga
     1321 ggttggtgaa attgtcgatg atgttcttca tctcgccgtc gtggaccacc cgcaccccgg
     1381 cggtgtcgag ccgcggcagc gcctcgtgca tgaaccgcgc cgttatctcg ggagccccgt
     1441 agcccggcgc gacatcggcc agcaccgaca ccgaatgctc cttgtcgaag cgatagatgt
     1501 agtccatctt ggaggtcagc gtgatctcgg cgatctgttt gagcaggacc ttgttgcccg
     1561 cgatggtcga ctttatcgcg agattgccca gctcctcgac gctgtcgata tccgattcga
     1621 cgatgatgtc gaactcgttg ccgccggtcc ggtagaccga cgccttgtcg ccgcgcaggg
     1681 cgaggttgat ctgccgctgg acgtcgtatt tgacgatgcc gaggctggtg gccacctcct
     1741 cgtcgacctt cacggtatat tcgaaccgtt cggaagcggc gttgtcgcgg acgttcatga
     1801 gccccggcac cgtccgcagt tcgtcctgca gggtgcgcga caccgtcagc aaccggcgca
     1861 ggtcctttcc ggtgagacgg aacatgaccg gcgcgtccat cggttcggcc tgctggagta
     1921 tcttcacctt cgccgagccg ccggtgacgg tcgcgtcgag tttctgctgg agatggtagc
     1981 cgaaccgttc gttgtcggcg aaacgtcccc ccttttcgag atcgacgcgg aacatcgcct
     2041 gtccatattc cttcgagggc ggctggggga acatcgtaac atagaatttc ggcaggtcgt
     2101 cgccgatgac cgtggtcatc tcggtcacct ccggttcgcc gcgcagg
//
LOCUS       DAOQUE010000485         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_64976_length_2141_cov_3.893576, whole genome shotgun sequence.
ACCESSION   DAOQUE010000485 DAOQUE010000000
VERSION     DAOQUE010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64976_length_2141_cov_3.893576"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKH10_12130"
     CDS             complement(<1..323)
                     /locus_tag="PKH10_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28910.1"
                     /translation="MKKILSAFLSLVALFFLLAACESSSSNTNDSDGTASDTLIDEDL
                     FSDDLVDEDEPVITDTKTDADLPADKDGIKDDGAPSDEDIVPPLDEDEPATDDIVTDD
                     VVTDD"
     regulatory      complement(354..473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(522..>2141)
                     /gene="pruA"
                     /locus_tag="PKH10_12135"
     CDS             complement(522..>2141)
                     /gene="pruA"
                     /locus_tag="PKH10_12135"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNT28911.1"
                     /translation="IFNLPESYNEPTLSYAPGTREKALLKAELKRQFDQKIEIPLIIG
                     GEEIRTGKTRKVVCPHDHRHVLAICHEAGENEVKMAIESALAAKQQWENTPWEDRAAV
                     FHRAASLISVKYRYILNAATMLGQSKSCYQAEIDATCETADFYRYNVKFMEQIYRNQP
                     ISDHHVWNRVHYRALEGFVYAVTPFNFTAIGANLPSAPAIMGNTVIWKPASTSLLSNY
                     YLMQLYKEAGLPDGVINFMPGAGAMISGIVLDHPALAGIHFTGSTDVFRSMWQTVGAN
                     LGKYRIYPRLVGETGGKDFIFMHQSADVDAVASAVVRAGFEYQGQKCSACSRVYVPRS
                     RWSELKEKLRSMVSMIKVGDVRDFRNFMNAVIDEASFDNTMRYIEIAKKSPDAEILTG
                     GTGDKSKGYFIQPTIIEAKRPRFITMEEEIFAPVVTVFVYDDDKLDETLDLVDSTSPY
                     ALTGAIFAKDRYLINKLSDALPNAAGNFYINNKPTGAMVGHQPFGGARASGTNDKAGS
                     FLNLIRWTSPRTIKECFTPPQVYEYPFLSEE"
BASE COUNT          404 a          608 c          644 g          485 t
ORIGIN      
        1 atgtcatcgg tcacaacatc atcggtgacg atgtcgtcgg tcgctggttc atcctcatca
       61 agcggcggaa cgatgtcctc gtccgagggg gcgccgtcat ccttgatgcc gtccttgtcg
      121 gcgggcaggt cggcgtcggt cttggtgtcg gtgatgaccg gttcgtcctc gtcaaccaga
      181 tcgtcactga acaggtcttc gtcgatcagg gtgtcgctcg cggtgccgtc ggaatcgttc
      241 gtgttactcg acgatgactc gcaggccgcg agcagaaaga agagagcgac gagcgacagg
      301 aaggcggaaa gaatcttttt catggcgatc actccttggt gtcgtttatg tccggtgatg
      361 catacacgct cctttccaaa agggaggcgc gggcctttcg gcaccgcacc ctgtcgaccc
      421 ggcatccata ccatttcggc ttcaggaacc cgggctcgga gtcgcatcag cctaccagaa
      481 tgtctcgaat aagcaagttt ttacggatgc ttccgcccgg ttcattcttc ggaaaggaac
      541 gggtactcgt agacctgcgg cggcgtgaag cattccttga tggtgcgcgg gctggtccag
      601 cggataaggt tgaggaaact gcctgccttg tcgttggtcc ccgaggcgcg cgcgccgccg
      661 aagggctgat gtccgaccat cgcgccggtc ggcttgttgt tgatgtagaa gttgcccgcc
      721 gcgttgggga gcgcgtcgct caatttgttt atcaggtaac ggtctttcgc gaaaatggct
      781 cccgtcagcg catagggcga ggtcgaatcg accaaatcaa gggtttcgtc aagtttgtcg
      841 tcgtcgtaca cgaacacggt caccaccggt gcgaagatct cttcctccat cgttataaag
      901 cgcggcctct tcgcctcaat aatggtcggc tgaataaaat aacccttcga cttatcgccg
      961 gtgccgcccg tcagtatctc ggcatcgggc gactttttcg cgatctcgat gtagcgcatg
     1021 gtgttgtcga aactcgcctc gtcgatgacg gcgttcatga aattgcgaaa gtcgcgtaca
     1081 tcgccgacct ttatcatcga gaccatggag cgcagtttct ccttgagttc gctccaccgc
     1141 gaccgcggga cataaacccg ggagcaggcg gagcatttct gcccctgata ctcgaacccg
     1201 gcgcgaacaa cggcgctcgc caccgcgtcg acatcggccg attggtgcat gaagatgaaa
     1261 tccttgccgc ccgtttcgcc gacgagccgt ggatagatgc ggtatttgcc gagatttgcg
     1321 ccgaccgttt gccacatcga cctgaacaca tcggtcgagc cggtgaagtg gatcccggcg
     1381 agcgccggat ggtcgagcac gatacctgat atcatcgcac cagcgcccgg catgaagttg
     1441 atgacaccgt caggtagtcc cgcctccttg tacagttgca tgaggtagta gttcgacagg
     1501 agcgatgtcg atgccggttt ccagatgacg gtgttgccca tgatcgccgg ggcggagggc
     1561 aggttggccc cgatcgcggt gaagttgaag ggtgtcacgg catagacgaa cccttcgagc
     1621 gcccggtagt ggacgcggtt ccagacatga tggtcggaga tgggttggtt gcggtagatc
     1681 tgttccatga acttgacatt gtagcggtaa aaatcggccg tttcgcaggt cgcgtctatc
     1741 tcggcctgat agcaggattt tgactgaccg agcatcgtcg ccgcgttgag gatgtagcga
     1801 tacttcaccg agatgagact cgccgcccga tggaacaccg ccgcccgatc ctcccacggc
     1861 gtgttctccc actgttgctt ggcggccagc gccgactcga tcgccatttt cacctcgttc
     1921 tccccggcct catggcagat agcgagaaca tgccggtggt cgtgcggaca gacgaccttt
     1981 ctggtcttcc cggtgcggat ctcctccccg ccgatgatga gcgggatctc gatcttctgg
     2041 tcgaactgcc tcttgagttc cgccttgagg agcgccttct cacgagtgcc gggggcatag
     2101 ctcaaggtcg gctcattgta ggattcaggc aaattgaaga t
//
LOCUS       DAOQUE010000486         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_65933_length_2121_cov_3.382381, whole genome shotgun sequence.
ACCESSION   DAOQUE010000486 DAOQUE010000000
VERSION     DAOQUE010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65933_length_2121_cov_3.382381"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PKH10_12140"
     CDS             <1..676
                     /locus_tag="PKH10_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28912.1"
                     /translation="DISMPERLTAQSEFLENNPGIGVLGAWMRTFGSRRRLMRYPEHD
                     ADIRIGNLFICSLGHPAVVLRKDAFAGKGLFFNGTLRAGVDYDLWSRAEAAGIRFHNL
                     QRPLVRYRTHAGQITSRRRDEQLATDRMVKRFLLERLGLTPSDDELDLHCGMMLRKTV
                     ISDVATADAYVAWTGRILDANRKSGVYDQAKLENYLRQWLTEACAAFVSRSGQALMAP
                     GLWSRG"
     gene            710..1831
                     /locus_tag="PKH10_12145"
     CDS             710..1831
                     /locus_tag="PKH10_12145"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNT28913.1"
                     /translation="MLNFKLSLPTEVFFGKGQIKVVGEQVRRHGGTRVLICYGSDRVK
                     RQGIFDTVTAKLADKGIALHELGGIAPNPRVTSVREGVKLVREHRCDFIVAIGGGSVL
                     DCAKGISAASGYEGAPWDLYTRKGEVKSVLPLGMVLTLAATGSEMNGTSVVTNLDTKQ
                     KLAIRLPMLKPRFSVLDPVYTYSVPPDQTAAGTVDIMSHVFEQYFSPVPGTFVQDRLA
                     DALLKTCLHYGPVALKEPENYEARANLLWAGTLALNELLTYGKTGDWATHMIGHGVAA
                     HYDVTHGVGLAILFPHWMQFVLDDGTQDKFYDLARNVWGVPLAMERMEAAREGIARTR
                     QFFTELGMPARLFFEPDYTDGGDDDTDPRTGGDAGTARG"
BASE COUNT          438 a          658 c          666 g          359 t
ORIGIN      
        1 cgacatcagt atgcccgaac ggctgacggc gcagagcgaa tttcttgaga acaacccggg
       61 tatcggcgta ctcggcgcat ggatgcggac cttcgggagc cgccggcggc tcatgagata
      121 ccccgaacac gacgccgaca tccggatagg caaccttttc atctgttccc tggggcaccc
      181 cgcggtcgtg ctgagaaagg atgcgttcgc agggaaaggg ctgttcttca acggaacgct
      241 tcgggccggc gtggattacg atctctggtc gcgcgccgag gcggcgggta tccggttcca
      301 taacctgcaa cggccgcttg tgcggtaccg gacccacgcg gggcagataa cgagcaggcg
      361 gcgcgacgaa cagttggcga ccgaccggat ggtgaaacgc tttcttcttg aacggctcgg
      421 cctgacgcct tcggatgatg aactcgacct tcactgcggc atgatgctgc gcaagacggt
      481 catcagcgat gtcgcgaccg ccgatgccta tgtcgcatgg acgggccgga tactggatgc
      541 gaaccggaaa agcggcgtgt acgatcaggc gaaactggag aactacctgc gacagtggct
      601 caccgaggcc tgcgccgcct ttgtctcccg atcggggcag gcgctcatgg cccccggtct
      661 ctggagccgc ggatgacccc ataaaccata ccacacagga ggaccgacga tgctcaactt
      721 caaactctcg cttcccaccg aggtcttttt cggcaagggt cagatcaagg tggtcggcga
      781 acaggtccgc cgccacggcg gcacccgcgt gctcatctgc tacgggtccg accgcgtcaa
      841 gcgacagggg atattcgata cggtgacggc gaaactggcc gacaagggga tcgcattgca
      901 cgaactcggc ggcatcgctc ctaacccgcg cgtcacgagc gttcgcgagg gggtgaaact
      961 cgtccgggag caccggtgtg acttcatcgt cgcgataggg ggcggcagtg tcctcgactg
     1021 cgcgaagggg atatcggcgg cctccggcta cgagggcgcc ccatgggatc tctatacgcg
     1081 gaaaggggag gtgaagagcg tcctgccgct cggcatggtg ctcacgctcg ccgcgaccgg
     1141 cagcgagatg aacggcacaa gcgtggtcac gaacctcgat accaaacaga aactggcgat
     1201 acgcctgccg atgctcaagc cgcgcttctc ggtgctcgac ccggtctata cctacagcgt
     1261 gccgcccgac cagacggcag ccgggaccgt ggacatcatg agccatgtgt tcgaacagta
     1321 tttctcaccg gtccccggca ccttcgtgca ggaccggctc gccgacgcgc tcctcaagac
     1381 ctgccttcac tacgggccgg tcgcgctcaa ggagccggag aactacgagg cgcgtgccaa
     1441 tctgctctgg gccgggacgc tcgccctcaa cgaactgctg acctacggca agacgggcga
     1501 ctgggcgacc cacatgatcg gccacggcgt cgccgcgcac tacgatgtga cacacggcgt
     1561 cgggctcgcc atccttttcc cgcactggat gcagttcgtg ctggacgacg gcacgcagga
     1621 taagttctac gatctcgccc gcaatgtctg gggcgtgccg ctcgcgatgg agcggatgga
     1681 ggcggcgcgc gaggggatcg cccgcacccg ccagttcttc accgaactcg gcatgccggc
     1741 gcggctcttc ttcgagccgg attacaccga cggcggtgac gacgacacgg acccgcgcac
     1801 gggaggggac gcaggaacgg cccgcggctg aaaacttttt acggccgcgc gggagaatgt
     1861 attgacactc cccgcgtccc gtagtacact atcattatca ttgcagcata ccgttcaacg
     1921 gcaattatat ctggaggacc agcgtatgaa caagagaact tttatcgcga tactggcggc
     1981 cgtagtcatt tccgcaaccc tctactcgca ggagaagaaa tcagaatcga aggaaggcgc
     2041 cgaggccgcg ggcaagcaga agatgaccgt gtaccaggaa ataatacttg aggatttcga
     2101 aaccacgccc tatacggata a
//
LOCUS       DAOQUE010000487         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_66191_length_2116_cov_4.249393, whole genome shotgun sequence.
ACCESSION   DAOQUE010000487 DAOQUE010000000
VERSION     DAOQUE010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66191_length_2116_cov_4.249393"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PKH10_12150"
     CDS             <1..438
                     /locus_tag="PKH10_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28914.1"
                     /translation="GDEQIPASAPYEEGDTSVRFSFPSPISIAAGESKHLVVTATLAL
                     ATDEKALIEIPKGGVELTTTATIAELPVRSKEFTASCIANDPTCTAGGGGGGGETGGD
                     EEEGGCGCALIADAGPGGGGALAGMAFLMFLLVLFPLRRLFSI"
     gene            508..>2116
                     /locus_tag="PKH10_12155"
     CDS             508..>2116
                     /locus_tag="PKH10_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28915.1"
                     /translation="MGRLSGIIILCAVIFPLTVMAQIGPDVSVYKGEQLKWEEGAHDF
                     FVMWKSLVARTDGSVDTGANNPQADSCVDPNVGSTYTLVDGTIPPNATVDRAFLVWMA
                     VVDPTQLNNPIKNSATLSFQHSTEPALSLTQEVTASVSGVLATTTTKDFEFEGLKYKD
                     PGGNDDGIFTYRVEVTDFMKKIQEDGNTLGMLDPTYALVGNYNVKGVPCSADQIYKTS
                     SGLMAGWSLVIIYTAEGVRAKKIYMYNGLMAYRYTSADIMIAGFQLPNEAEIRATLFV
                     GEGDPGLFDALNGNLEGLQLHGPIDQSAPWAPIFNVCNPQVNNYYEIYNSISSLYPWG
                     AQDPVCIGDFASKQLEYAMDVDTFLIRMKDYPFSDHLKKDDGVFWLKIGANQDQVYSN
                     FLVLSIDTKPPSFDIPEGTEFATDGREKALCTCAPVNEPDRICPDRPFYYLLKIQNWG
                     ENVADNVKVQDTLPPEIEYIPGTTEMATEFAGISGTNWTSIPDSGGFPLNSPFEVHPS
                     MGYCDPNTKVCPNTVYVRFKVRPKPDLP"
BASE COUNT          438 a          653 c          631 g          394 t
ORIGIN      
        1 ggcgacgagc agatacctgc ctcggccccg tatgaagagg gcgacacctc ggtccgcttc
       61 tcgttcccga gccccatatc gatcgccgcc ggggagtcga aacatctcgt ggtgaccgcg
      121 acgctggcgc tggcgaccga cgagaaggcg ctcatcgaga tacccaaggg cggcgtcgag
      181 ttgacgacga ccgccacgat agccgaactg ccggtgcggt ccaaggaatt cacggcctcc
      241 tgcatcgcga acgacccgac ctgcacggcg ggaggaggcg gtggcggcgg cgagaccggc
      301 ggggacgagg aagagggcgg ttgcggatgt gcgctcatcg ccgacgccgg tccgggagga
      361 ggaggcgccc ttgccggaat ggcgttttta atgttcctgc tggtcctttt tcccctgcgc
      421 cgtctatttt ccatttgact tttgccccct tccggttcta gaatcgcccg taaggttctt
      481 ttgatgttcg acgatatgga ggtcatcatg ggtcgcttgt ccggtatcat tatcctttgc
      541 gcggtcatct ttccgctgac cgtcatggct cagatcgggc ccgatgtgtc ggtgtacaaa
      601 ggtgagcaac tcaagtggga ggagggggcg cacgatttct tcgtcatgtg gaaatcgctc
      661 gtcgcccgta ccgacggctc ggtcgatacc ggcgcgaaca acccgcaggc cgattcctgc
      721 gtcgatccca atgtcgggag cacctatacg ctggtggacg gtacgatacc gccgaacgcc
      781 acggtcgacc gcgccttcct cgtctggatg gcggttgtcg acccgacgca actgaacaac
      841 ccgataaaga acagcgcgac cctctcgttc cagcacagca ccgaaccggc gctgtcgctc
      901 acgcaggagg tgacggcgag cgtgtcgggg gtgcttgcga ccactacgac caaggatttc
      961 gaattcgagg gacttaaata caaagacccg ggcggcaacg acgacggcat cttcacctat
     1021 cgcgtggagg tgaccgactt catgaagaag atacaggagg acggcaacac cctcggcatg
     1081 ctcgacccga cctatgcgct cgtcggcaac tacaatgtca aaggggtgcc ctgttcggcc
     1141 gaccagatat acaagacctc gagcggtctc atggcgggct ggtcgctcgt catcatctac
     1201 accgctgaag gcgtccgcgc caagaagata tatatgtaca acgggctcat ggcctaccgc
     1261 tatacctcgg ccgacatcat gatcgcgggc ttccagttgc ccaacgaggc cgagatacgg
     1321 gcgaccctgt tcgtgggcga gggagacccg ggcctgttcg acgctctgaa cggcaacctc
     1381 gagggcctgc agctgcacgg cccgatagac cagagcgcgc cgtgggcgcc gatattcaac
     1441 gtctgcaacc cgcaggtgaa caactactac gagatataca actcgatatc gagtctctat
     1501 ccgtgggggg cgcaggaccc ggtctgcatc ggtgatttcg catcgaagca gctcgagtac
     1561 gcgatggatg tcgatacctt cctgatacgg atgaaggact acccgttcag cgatcatctt
     1621 aaaaaggacg atggcgtgtt ctggctcaag ataggggcca atcaggatca ggtctacagt
     1681 aacttcctcg ttctctcgat agacacgaaa ccgccctcgt tcgatatccc ggaagggacc
     1741 gagttcgcca ccgacggccg cgagaaggcg ctctgcacct gcgcgccggt caacgagccg
     1801 gaccggatct gccccgaccg cccgttctac tatctgctca agatacagaa ctggggcgag
     1861 aatgtcgccg acaacgtaaa ggtgcaggac accctgccgc ccgagataga atacatcccc
     1921 ggcaccaccg agatggcgac cgagttcgcc gggatctccg gcaccaactg gacctcgata
     1981 ccagacagcg gcgggttccc gctcaattcg ccgttcgagg tccatccctc gatggggtac
     2041 tgcgatccga acacgaaggt ctgcccgaac accgtctatg tccgtttcaa ggtgcgtccc
     2101 aaacccgatc tgccca
//
LOCUS       DAOQUE010000488         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_66390_length_2112_cov_3.966942, whole genome shotgun sequence.
ACCESSION   DAOQUE010000488 DAOQUE010000000
VERSION     DAOQUE010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66390_length_2112_cov_3.966942"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PKH10_12160"
     CDS             complement(<1..858)
                     /locus_tag="PKH10_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT28916.1"
                     /translation="MKRMFVFIGVIAAFAALSAEEPRLATVIFTNDTHGMAWAYDEPD
                     NPGVGGLAAAKTVIDRIREEAQLGNGDTVLVSSGNITMGDPRSNICENVPLIKGMSLI
                     GYDAMAVSNHEFDFGLKVFRKMQKEANFPFLSVNLFEKGQKKTMLQEFVEKKLSNGLK
                     VAMLAVTTPETAKITEVGLKGEVTVDDPIARAKKLATELKARNDVVIVLSNLGYYESD
                     RSFDGYPADRLLAKNLAGVADLIVGGHTKTHLEQPVVEAGIPIVQTEGLGKWVGRFDL
                     YILGGKVTQT"
     gene            complement(944..1675)
                     /gene="def"
                     /locus_tag="PKH10_12165"
     CDS             complement(944..1675)
                     /gene="def"
                     /locus_tag="PKH10_12165"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNT28917.1"
                     /translation="MKGLFALLPMAFLLVSCATAAPAPCPQPTVTAPAAVAHQPLDRY
                     ERRIITAGAPAEVMALVENDTPSDDAFLREKALTVDPAEPIIGHLAARMLETVKKAPG
                     VGIAAPQVGISRRLIWVQRMDKEEKPFEFYINPRIDEFSPEKEIGWEGCLSVPAGFGK
                     CYRSTSITLSHDTTDNGRVSEKISGFTAVIFQHEIDHLDGVLFIDLKEQGDLMPKEAY
                     YEMRRKEKEAKEKEQAAPAATPENK"
     gene            complement(1693..>2112)
                     /locus_tag="PKH10_12170"
     CDS             complement(1693..>2112)
                     /locus_tag="PKH10_12170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT28918.1"
                     /translation="LQQHLLLADRYERTSALVMFSVDQYGDLLANHGSDRAEKVLGLL
                     AELVRQNTRRSDAAARIDKDRFALLLPETDLPRAMKIAEKIRGEVEAFHFGPEPLTLT
                     AGVTQIEQGESADMAIARVESAWRKALRSDDKKISAL"
BASE COUNT          357 a          615 c          675 g          465 t
ORIGIN      
        1 ggtctgcgtc accttgccgc cgaggatgta gagatcgaag cgtccgaccc acttgccgag
       61 cccttcggtc tgcactatcg ggatacccgc ctcgaccacc ggctgttcga ggtgggtttt
      121 ggtgtgacca ccgacgatga gatcggcgac gcccgcgaga ttcttagcca gcagtctgtc
      181 ggccggatag ccgtcgaagg acctgtcact ttcgtaatag ccgagattcg acagtacgat
      241 gaccacatcg ttgcgtgcct tgagttcggt cgccagtttc ttggctcgtg ctatcgggtc
      301 atcgaccgtg acctctccct tgagcccgac ctcggttatc ttggccgttt caggggtggt
      361 gacggcgagc atggcgacct tgagcccgtt ggagagtttc ttttcgacga actcctgcaa
      421 catcgtcttt ttctgtccct tctcgaacag gttgaccgaa aggaagggga agttcgcctc
      481 cttctgcatc ttgcggaaca ccttgagtcc gaagtcgaac tcgtggttgc tgaccgccat
      541 cgcgtcgtaa ccgatgagac tcatcccctt gatgagcggg acattctcgc agatgttgga
      601 ccgcgggtcg cccatggtga tgttgcccga cgaaacgagc accgtgtcgc cgttccccag
      661 ttgcgcctcc tcgcggatgc ggtctatcac cgtcttggcc gccgcaagtc cgccgacgcc
      721 cgggttgtcg ggctcgtcat aggcccacgc catgccgtgc gtgtcgttgg tgaagatgac
      781 ggtcgcgagc cgcggctcct cggccgacag agccgcgaac gcggcgataa caccgataaa
      841 aacgaacatt cttttcatag cgatccccct catgtaaagt tcacggaacg accatagcgg
      901 aaagcggggg aaaggtcaaa aaaacagtaa aaaggaagag gggctatttg ttctcgggtg
      961 tcgcggcggg agccgcctgt tctttttcct tcgcctcttt ttccttgcgg cgcatctcgt
     1021 agtatgcctc tttgggcatc aggtcgccct gttccttgag gtcgatgaag aggacgccgt
     1081 cgaggtggtc tatctcatgc tggaatatga cggcggtgaa gcccgatatc ttttccgata
     1141 cccgtccgtt gtccgtggtg tcgtgcgata gggttatgga ggtcgatcgg tagcatttgc
     1201 cgaagccggc ggggaccgac aggcaccctt cccagccgat ctctttttcg ggcgagaatt
     1261 cgtctatgcg cgggttgatg tagaactcga agggtttttc ctccttgtcc atccgctgga
     1321 cccagatgag gcgacggctg atgccgacct gcggcgcggc gatgccgacg ccgggcgcct
     1381 tcttgaccgt ttcgagcatc cgcgcggcga gatggccgat gatcggctcg gccggatcga
     1441 ccgtcagcgc cttttcgcgc aggaaggcgt cgtcggacgg cgtatcgttc tcgacgagcg
     1501 ccatgacctc ggccggggcg cctgcggtga tgatccgccg ttcatagcga tcgagcggct
     1561 gatgggcgac cgcggcggga gcggtgacgg tcggttgcgg gcagggggcg ggagcggcgg
     1621 tcgcgcacga aacgagcagg aacgccatgg gaagcagtgc gaaaagacct ttcatggggg
     1681 ttctccgggt ttttagagcg ccgatatctt cttgtcgtcg cttcgcaggg ctttccgcca
     1741 cgcgctctca acgcgggcta tcgccatgtc ggccgattct ccctgttcta tctgagtgac
     1801 gccggcggtc agcgtgagcg gctccggacc gaaatggaac gcttcgacct cgccccgtat
     1861 cttttcggct atcttcatgg cacgcggcag gtcggtctcg gggagcagga gggcgaaccg
     1921 gtccttgtct atccgggcgg cggcgtcgga gcggcgggtg ttctgccgca caagttcggc
     1981 gagcaggccc aatacctttt cggcccggtc gctaccgtgg ttggcgagca gatcgccgta
     2041 ttgatcgaca ctgaacatga caagcgccga ggtccgttcg tagcggtcgg cgagcaggag
     2101 atgctgctgc ag
//
LOCUS       DAOQUE010000489         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_66395_length_2112_cov_3.467185, whole genome shotgun sequence.
ACCESSION   DAOQUE010000489 DAOQUE010000000
VERSION     DAOQUE010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66395_length_2112_cov_3.467185"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PKH10_12175"
     CDS             complement(<1..287)
                     /locus_tag="PKH10_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT28919.1"
                     /translation="MNGAIDKKELILNGFITLVGEYGINRVTLADVAERAGLTKSALY
                     YYFESKEALLVEGFELFNTKLKERIDPLVAQAKNPREVLWIYCDFNLKVFFG"
     gene            complement(300..>2112)
                     /locus_tag="PKH10_12180"
     CDS             complement(300..>2112)
                     /locus_tag="PKH10_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT28920.1"
                     /translation="LSALDATQEIGFAVLAITLTIVAVFGPIVYMTGMVGKIMRQFGA
                     TIAISVMISLLISFTVTPLLSSLMLKEESKKFFLYRGMEALLVWLENGYSKMIALVLR
                     NRLTKVTVFATGVLLFAAGLMLATVLKTNFMDKMDQGDFDINIELAGEASIDRSKEVA
                     EDVVRVVTQKPWVDFTFTTIGGGQRNEKNKVVLRVKMVDRHQRSITQMEAMDEMRGDL
                     AYLETKYGAVLGVAEKNEFGGGGMMPIMFNIIGPDYEEIRRDAAGLISFMEHDGGYTD
                     INSSDKGQKKELRIRVDHEKAAELGVSPFETSMALRYLFSGEKIANFREGGDMYDIKV
                     YLDPDYKSIDYIRDLPLKGSNGRIARVADVAEVTYGASEVIITRMERNRLITVTADYA
                     SSTSLGVQVEKAKTYAKTNFSPRNRLTMGGEAEMMEDTFASLFRTMFISIFLIYIILA
                     SQFNSFIHPLTIMSAIPFAITGAFFSLWVTGMSLSMMSFIGVIMLMGLVTKNSILLVD
                     FSIERMRHGMTAAQALAEAGRIRLRPILMTTLAIIFGMIPVAIAAGEGAEVKHPMAWS
                     VMGGVIFSTIVTLFIVPVIFSFFDMFRKHDAQKPDTV"
BASE COUNT          448 a          619 c          586 g          459 t
ORIGIN      
        1 ccgaagaata ccttgaggtt gaaatcgcag tagatccaca acacctcgcg ggggtttttc
       61 gcctgcgcga cgagagggtc gatccgctcc tttagtttgg tattgaacaa ttcgaaaccc
      121 tcgaccagga gcgcttcctt ggattcgaag tagtagtaga gcgccgactt agtgagtccc
      181 gcccgctcgg cgacatcggc gagcgttacg cggtttatcc cgtattcccc caccaacgtt
      241 atgaagccgt tgaggatgag ttccttcttg tcgatggcac cgttcatcgc agtctccctt
      301 cagaccgtat cgggcttctg agcgtcatgt ttgcggaaca tatcgaagaa actgaatatc
      361 accggaacga tgaagagggt gacgatcgtc gagaagatga ctccccccat gaccgaccac
      421 gccatcggat gctttacttc agccccctcg ccagcggcga tggcgaccgg tatcatgccg
      481 aagatgatgg cgagcgtcgt catgaggatg ggccgcagtc ttatccgccc tgcttcagcg
      541 agcgcctgcg ccgccgtcat cccgtgccgc atccgctcga tggagaaatc gacgagcagg
      601 atcgagttct tggtgacgag gcccatgagc atgatgacgc cgatgaaact catcatcgac
      661 agcgacatac ccgtcaccca caggctgaag aaggcgccgg tgatggcgaa cggaatagcc
      721 gacatgatgg tgagcggatg gatgaatgag ttgaactgcg aggcgagtat gatgtagatg
      781 aggaatatcg atatgaacat cgtccggaaa agcgacgcga aggtatcctc catcatttcg
      841 gcctcgcccc ccatcgtcag ccggttcctc ggcgagaagt tcgtcttggc ataagtcttt
      901 gccttttcca cctgcacgcc caacgaggtc gacgaggcat aatcggccgt caccgtgatg
      961 agccggttcc gttccatccg cgtgatgatg acctccgagg caccgtaggt gacctcggcc
     1021 acatcggcga cacgggcgat ccggccgttg ctgcccttga ggggcagatc gcggatgtag
     1081 tcgatcgatt tatagtccgg gtcgagatag accttgatgt cgtacatgtc gcccccctcg
     1141 cggaaatttg cgatcttctc gcccgagaag agataacgca acgccatgga tgtctcgaac
     1201 ggactgacgc cgagttcggc cgctttctcg tgatctaccc gtatccgcag ctcttttttc
     1261 tgtcccttgt cggagctgtt gatgtcggtg tagccaccgt cgtgctccat gaacgagatg
     1321 agccccgccg cgtcgcgccg gatctcctcg tagtcaggac caatgatgtt gaacatgatc
     1381 ggcatcatac caccgccgcc gaattcgttc ttctcggcaa cgcccaagac agcgccatat
     1441 ttcgtttcca gataggcaag atcgccacgc atctcgtcca tcgcctccat ctgcgtgatg
     1501 ctccgttggt gccggtcgac cattttgacc cgtagaacca ctttgttctt ctcattacgc
     1561 tggccgccgc cgatggtggt gaaggtgaaa tcgacccacg gtttttgcgt aacgacccgc
     1621 actacatcct ccgccacctc cttggaacga tctatcgacg cctcacccgc gagttcaatg
     1681 ttgatgtcga agtcgccctg atccatcttg tccatgaagt tggtcttgag caccgtggcg
     1741 agcatcaggc cggcggcgaa cagcaatacc ccggtggcga agaccgtcac cttggtcaag
     1801 cgattacgca ataccagcgc tatcatcttc gaatagccgt tctcgagcca caccagtagc
     1861 gcctccatcc cgcggtagag gaagaacttc tttgactcct ccttgagcat gagcgacgaa
     1921 aggagcggcg taacggtgaa tgagatgagc agtgatatca tgaccgatat cgcgatggtc
     1981 gcgccgaact gccgcatgat cttgccgacc atgccggtca tgtagacgat ggggccgaag
     2041 accgcaacga tggtgagcgt tatcgcgagg accgcgaacc ctatctcttg ggtggcgtcg
     2101 agcgccgaaa gg
//
LOCUS       DAOQUE010000490         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_66807_length_2104_cov_3.270864, whole genome shotgun sequence.
ACCESSION   DAOQUE010000490 DAOQUE010000000
VERSION     DAOQUE010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66807_length_2104_cov_3.270864"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKH10_12185"
     CDS             <1..246
                     /locus_tag="PKH10_12185"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HNT28921.1"
                     /translation="AVRWIGNFTFAGGRYRFTTVTDDGVRIYLDDVLILDSWRPMRGT
                     RSAVKTIAEGEHTVRVEYFEAMEAAKAQVYWTWLGSR"
     gene            613..750
                     /locus_tag="PKH10_12190"
     CDS             613..750
                     /locus_tag="PKH10_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28922.1"
                     /translation="MGKGGGGKGGGGGKGGGGKGGGNPNWPSTTGNPSGGGRGNNPPG
                     K"
     gene            892..1335
                     /locus_tag="PKH10_12195"
     CDS             892..1335
                     /locus_tag="PKH10_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT28923.1"
                     /translation="MSMIKRYEHPERNLMSRQYGGLFGDLFSDLFDNALGYAVSENWP
                     KVDIEETDEAYLIKAELPGMSKDQVQIEIRNNVLSLSGEKKETREEKSKNVHRRESYI
                     GGFCRNFSLPDQVATDKVDAEFRDGVLRLAIPKSEERKPRKITIR"
     gene            1464..2057
                     /locus_tag="PKH10_12200"
     CDS             1464..2057
                     /locus_tag="PKH10_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28924.1"
                     /translation="MNAKVGIAIAVVFTIVWIGFKMMSPSADEKYARFENQGAPSKGG
                     AAAKPEFDAAESYTFTLDATGLELKDDLRDALLAELRRAVAPAKVNPVTGPKAGKGDL
                     GAITATVTVETKTYAGQQAAIRHKSGQMPYKIQGTLTVESAAGRSSWDGTRTLAAEVA
                     PPDEVRDGIFYDIVDKQCRELARRAVDAIREKEPFTR"
BASE COUNT          472 a          591 c          663 g          378 t
ORIGIN      
        1 gcagtacgct ggatcggcaa cttcaccttc gcaggcggtc gctaccgctt caccaccgtg
       61 accgatgatg gcgtgcggat ttacctcgat gatgtgttga tcctcgatag ctggcgcccc
      121 atgcgcggta cccgcagcgc cgtgaaaacg atcgctgaag gcgagcatac ggttcgcgtc
      181 gagtacttcg aggcgatgga agctgcaaaa gcacaggtat attggacttg gctggggagt
      241 agatgagcgt agaagaaacc tatcccggat tgcatcccgt ccgccagcgc cccgtgtcgt
      301 cacgggggct ggcggctgtg gtggttttgc tcctcgccgt gctgacgctg atcgccgcgt
      361 gtgctgaaga tgacggtaac ttctacgccg gactcgaatg gaccatgaga ctgagaacgg
      421 ggtcagtcct cgatattaga cacgagagag taagtccttc ctttctcacc caataaaaaa
      481 gaaaagattt tttcgaatag attttttagt ccacgctctc tagtgtgttt ttgccaaaac
      541 cagggtatga ttccgattcg gtccggaacg gaacggagtc cataaaattt ccggaaataa
      601 gaggaggcta tcatgggtaa aggtggcggt ggcaaaggtg gtggcggtgg aaagggcggc
      661 ggcggcaaag gcggcggcaa cccgaattgg cccagcacaa cgggcaatcc ttctggcggt
      721 ggtcgcggga acaatcctcc gggcaagtaa cagtaccgat cagtttcaga ttcattcctt
      781 catttcccca taaccccctt gacagaaaaa aactcgtgcc tatctaaaaa gtcgtgaccg
      841 gttctttgtg aataacctga ataaccttaa cctgttggat ggaggagtac catgtcgatg
      901 atcaagaggt atgagcaccc cgaacggaac ctgatgagcc gccagtacgg cggactgttc
      961 ggcgatctgt tcagcgacct gttcgacaac gcgctgggct acgccgtgag cgagaactgg
     1021 ccgaaggtcg acatcgagga gaccgatgag gcatacctca tcaaggcgga gttgccgggc
     1081 atgtcgaagg atcaggtgca gatcgagatc cgcaacaatg tgctgtccct ttcgggcgag
     1141 aaaaaggaga cgcgcgagga gaagagcaag aatgtccacc ggcgcgaatc gtacatcggc
     1201 gggttctgcc gcaacttctc attgcccgat caggtggcga ccgacaaggt cgacgccgag
     1261 ttccgcgacg gcgtcctgcg gctggccatc cccaagagcg aggagcgaaa gccgcggaag
     1321 ataacgatcc gctgaacatt tcaccaaccc ccaccccgag aacggccccc gctcccgcgg
     1381 gggcttcctt tttcagggag agggaatttg ccctcgcctt gcgtatatgc tataacagcc
     1441 cgacctaagt gagggagggg accatgaacg ccaaggtcgg tatcgccatc gccgttgttt
     1501 tcaccatcgt ctggatcggc ttcaagatga tgagcccgtc ggccgacgag aagtacgccc
     1561 gtttcgagaa ccaaggggcg ccgtccaagg gcggcgcggc cgccaagccc gagttcgacg
     1621 cggccgagag ttacaccttc acgctcgacg cgaccgggct cgaactgaag gacgatctgc
     1681 gcgacgccct gctcgccgag ttgcgccgcg ccgtcgcgcc ggcgaaggtg aacccggtca
     1741 ccggcccgaa ggcggggaag ggtgatctgg gcgcgatcac cgcgacggtg acggtcgaga
     1801 cgaagaccta cgcgggacag caggcggcga tacggcacaa gagcggtcag atgccctaca
     1861 agatacaggg gacgctcacg gtcgaaagcg ccgcggggcg cagttcgtgg gacggcacgc
     1921 ggacgctcgc cgccgaggtg gcgccgcccg acgaggtgcg cgacgggata ttctacgata
     1981 tcgtcgacaa gcagtgccgc gaactggcgc gccgggcggt ggacgccatc cgggaaaagg
     2041 aaccgttcac acggtgagtc gtggaaacgg cgggttcagc tccgcatctt ccagctaacc
     2101 gacc
//
LOCUS       DAOQUE010000491         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_67282_length_2094_cov_3.601766, whole genome shotgun sequence.
ACCESSION   DAOQUE010000491 DAOQUE010000000
VERSION     DAOQUE010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67282_length_2094_cov_3.601766"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PKH10_12205"
     CDS             complement(<1..1100)
                     /locus_tag="PKH10_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNT28925.1"
                     /translation="MEKNRSFLSKFFNTTALAHFTLGNIVMMIIAFFFLWFAIGKGYE
                     PLLLLPIGFGILLGNIPSAAGVQIGVYDPGSVLNYLYFGVLKGIYPPLIFLGIGAMTD
                     FSYMISNPKLILLGGGAQLGIFMAFLIAIFLGFNLQEAASIGIIGGADGPTAIFVTGL
                     MAPYLLGAVAIAAYSYMALVPVIQPPVVNLLTTKKERLIRMKPPRTVSKREKIIFPIV
                     AVIICAIIAPGGLPLIGLLLLGNILKESGVTDRLAKTAGSALIDIVTILIGLTVGAST
                     SAQTFLTGKSVMIFALGALSFMFSTAGGVLIAKFINLFLKEGNKINPIIGCAGVSAVP
                     DSARVAQHMGRHEDPENHLLMQAMAPNVAGVIG"
     gene            complement(1659..>2094)
                     /locus_tag="PKH10_12210"
     CDS             complement(1659..>2094)
                     /locus_tag="PKH10_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNT28926.1"
                     /translation="MKKYDLKINGKDYKVEVKEFGAKSAKVEVNGKPFSVDISYPAGE
                     TAPAIPRPVAPRAAAVAPVVQAAPAPAAAAGNSVNAPMPGLILKLLVKVGDRVAVGQK
                     VAVMEAMKMENDINTTFAGTVAEIRVVEGANVKEHDPLIVIA"
BASE COUNT          428 a          602 c          644 g          420 t
ORIGIN      
        1 ccgataacgc cggccacatt gggcgccatg gcctgcatga gcaggtgatt ctccggatcc
       61 tcgtgacgcc ccatgtgctg ggcgacccgc gcgctgtcgg ggaccgccga gacaccggcg
      121 cacccgatga tcgggttgat cttattcccc tctttcagga aaagattgat gaacttcgcg
      181 atgagcacgc cacccgccgt agagaacata aaagagaggg cgccgagcgc gaatatcatg
      241 accgatttgc cggtcaggaa ggtctgcgcc gaggtcgagg caccgaccgt gagtccgatg
      301 agtatcgtca cgatatcgat gagcgcgcta cccgccgtct tggcaaggcg atcggtgacg
      361 cccgattctt tcaggatgtt gccgagcaga agcaggccta tgagcggcag gccgccgggg
      421 gcgatgatgg cacagatgat caccgcgacg atcgggaaga ttatcttctc tcgtttcgag
      481 acggtgcgtg gcggcttcat ccggatgagc cgttctttct ttgtcgtcag caggttgacc
      541 accggcggct ggatgaccgg aacaagcgcc atgtaggagt aggccgctat cgcgaccgcg
      601 ccgagcagat agggtgccat gaggccggtg acgaatatcg ccgtcgggcc gtcggcgccg
      661 ccgatgatgc cgatggatgc cgcttcctgc aggttgaatc cgaggaatat cgcgataagg
      721 aaggccatga atatgccgag ttgcgcgccg ccgccgagta ggataagttt cgggttcgat
      781 atcatgtagg agaaatcggt catcgcaccg atgccaagga aaatgagcgg cggatagatg
      841 cctttcaaga cgccgaaata gagataattg agcaccgaac cgggatcata gacgcctatc
      901 tggacgccgg ccgccgaggg tatattgccg agcaggatgc cgaagccgat gggcaacagc
      961 aggagcggct cgtaaccctt tcctatcgcg aaccagagaa agaagaaggc gatgatcatc
     1021 atcacgatat tgccgagcgt aaagtgggcg agcgcggtgg tgttgaagaa tttcgaaagg
     1081 aaagagcggt tcttttctat atagtattcg agcgtgcccg acgaacggag cgagaagtcg
     1141 atgaacagcg gggtgatgtt gtaccggctc ttgaaattgg tgatgtagag cgagttctcc
     1201 aactggacat tgcctacctg ctcacccggt atcgcgaggg gataacgccc cggctctccc
     1261 ttgaagctca cgaaggcgac gccgttgtcg gcgacgatca gagcggtgtg gtccagttcg
     1321 agcgtcgctc cgtcggggaa ggtcagcgtc atccgatagt tcttcccgtc gggcatgaag
     1381 atatcgatgt ggtcctcttc gggaacgacc gctcgctgcg aggtgaccat gagatattcc
     1441 ttcttcgtat cgtcccgtgc gacgatggcg ccgttctcgc gccgttcgac ccacgcgggc
     1501 tgcccatcac tgccgagcgt ctggtaacgg atgtccagtt tcaccagcga accgtttacg
     1561 gctgtgccgg tgaactgcgt ctcctgcgcc gccagcaaca ccgacgaaag gatcaggagc
     1621 acaaagagcg gtatctggat cgatctcatg acggactcct acgcaatgac gataagcgga
     1681 tcatgctcct tgacattggc cccctcgacc acccgtatct cggcaacggt cccggcgaag
     1741 gtggtattga tgtcgttctc catcttcatc gcctccatca cggcgacctt ctggccgacc
     1801 gcgaccctgt cgcccacctt gacgaggagt ttgaggataa gtcccggcat cggagcgttg
     1861 acactgttgc ccgcggccgc cgccggagcc ggcgccgcct gcacgaccgg agcgaccgcc
     1921 gccgctctcg gcgcgacggg gcgcgggatg gccggagccg tctcaccggc cggataggag
     1981 atatcgacgc tgaagggttt gccgttcact tcgaccttcg cgctcttcgc gccgaattcc
     2041 ttcacctcga ccttgtagtc cttgccgttt atcttcagat catacttttt catc
//
LOCUS       DAOQUE010000492         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_67663_length_2087_cov_3.512303, whole genome shotgun sequence.
ACCESSION   DAOQUE010000492 DAOQUE010000000
VERSION     DAOQUE010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67663_length_2087_cov_3.512303"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="PKH10_12215"
     CDS             <1..1367
                     /locus_tag="PKH10_12215"
                     /inference="COORDINATES: protein motif:HMM:NF026037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28927.1"
                     /translation="VKGDYYCTDACDPLAADACDDGYVCEGITDQENRTACVTKVYLK
                     GKVFDLETDAAIKDALVMAQNTKDGIATDVVKTAADGSWTIEIAVPRNSHGDPATGDI
                     YTLYASADDHIPYPSLMRPALPVTLTQFDPETADLPSSAWEYSSELTDIGLLPVKDEE
                     KGRASINGKLANPFGGVLIVAECAAPPCPYNYADKKGNFTIFNVAPGNYELAAYKRGV
                     YCEKKTVTVAATDITGLTLAAKEFAGGSVSGSVNIVNAPGGSKTSVVLIPESTFHETL
                     VTGVVAPGLRAPQPPAAPNINGAFEITDVPEGKYVVLAAYENDGLVRDPDPNIAGTQI
                     VHFEITITATTKTLDSFKITEALAMVSPGGSTPEAVSGTPTFKWADDSSEDAYNLKVY
                     NIYGDLVWEKDIDGVSGSETVEVPYEGPALEAGMYYQWKAVSYRKDGPISTTEDLRGV
                     FYAE"
     gene            complement(1370..>2087)
                     /locus_tag="PKH10_12220"
     CDS             complement(1370..>2087)
                     /locus_tag="PKH10_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28928.1"
                     /translation="ATFLDAMPSEWDAFFNLRMIGLINDPNTMSLDAAFLVDLGATLK
                     GATYDLGDTQGYYLRDAVETNGGSSVQTVIATGLGNLTWPVSGQLASLWVGQLYAAVD
                     DLMDYKSQAANEGVGGVTVEGSFKMTVFEIWIEIIGSSQVTRMECVRGISAMNGDGTA
                     YEGSRFVCVDNNTEWAIGETMKMMDYAQMLDQDADILAVLNEGLAENDPDYRADVCQC
                     WEQDGTTLMNCDDMRIEFGL"
BASE COUNT          452 a          687 c          592 g          356 t
ORIGIN      
        1 gggtgaaggg cgactattac tgcaccgatg cctgcgaccc gctcgccgcc gacgcctgcg
       61 atgacggcta tgtctgcgaa gggataaccg atcaggaaaa tcgcaccgcc tgcgtgacaa
      121 aggtctatct gaaggggaag gtgttcgacc tcgagaccga tgcggccatc aaggatgcgc
      181 tcgtgatggc gcagaacacc aaggacggca ttgcgaccga tgtggtaaag acggcggccg
      241 acggctcgtg gaccatcgag atagcggtgc cgcgcaacag ccacggcgac ccggcgaccg
      301 gtgacatcta cacgctctac gcatcggccg acgaccacat tccctacccc tcgctcatgc
      361 gtcccgccct gccagtgaca ctcacgcagt tcgaccccga aacggccgac ctgccatcga
      421 gcgcgtggga atattcctcc gaactgaccg atatcggcct cctgccggtc aaggatgagg
      481 aaaaaggacg tgcctcgatc aacggcaaac tcgcgaaccc cttcggcggc gtacttatcg
      541 tggccgaatg cgccgcaccg ccgtgtccct acaactacgc cgacaaaaaa gggaacttca
      601 ccatcttcaa cgtggcgccg gggaactacg aactggccgc atacaaacga ggcgtctact
      661 gcgagaagaa gacggtgacg gtcgccgcga ccgacatcac cggcctcacg ctcgccgcca
      721 aagagttcgc cggcggctcc gtgagcggat cggtcaacat cgtcaacgcc ccgggcggct
      781 cgaagacctc ggtggtcctc atccccgaat cgacctttca cgaaacgctc gtgaccggcg
      841 tggtcgcccc cggtctgcgc gccccccaac cgcccgccgc gccgaacatc aacggcgcct
      901 tcgagataac cgacgtaccg gaaggaaagt atgtggtgct ggccgcctac gagaacgacg
      961 gcctcgtgcg cgaccccgac ccgaacatcg ccgggacgca gatcgtccac ttcgagataa
     1021 caatcaccgc cacgacaaag acgctcgaca gtttcaagat aaccgaggcg cttgcgatgg
     1081 tatcgcccgg cggctccacc ccggaggcgg tcagcggcac cccgaccttc aagtgggccg
     1141 acgattcgag cgaggacgcc tacaatctca aggtctacaa catctacggc gatctggtct
     1201 gggaaaagga tatcgacggg gtcagtggat cagaaacggt cgaggtcccc tacgaaggac
     1261 cggcgcttga agcgggcatg tactaccagt ggaaagcggt ctcctaccgc aaagacggcc
     1321 ccatatcgac caccgaggac ctccgcggcg tcttctacgc tgagtgatcc tatagcccga
     1381 actctattct catgtcatcg cagttcatca gggtcgtccc gtcctgttcc cagcactgac
     1441 agacatcggc acggtagtca ggatcgttct cggcaagtcc ctcgttgagg acagcgagga
     1501 tatcggcatc ctgatcgagc atctgcgcgt agtccatcat cttcattgtc tcgccgatcg
     1561 cccattcggt gttgttgtcg acgcaaacga accgcgagcc ttcataggcc gtcccgtcgc
     1621 cgttcatcgc cgaaatgccg cgcacacatt ccatgcgggt cacctgagag gagccgatga
     1681 tctctatcca tatttcaaat acggtcatct tgaaggaacc ttcgaccgtg acgccgccga
     1741 ccccctcgtt cgccgcctgc gacttgtagt ccatgaggtc gtccacggcg gcgtacaact
     1801 ggccgaccca caacgaggcc aactggccgg aaacgggcca agtcaggttg ccgagccccg
     1861 ttgcgatgac cgtttgaacg gacgagccgc cgttcgtttc caccgcatca cgcagatagt
     1921 acccctgcgt atcgcccaga tcgtaggtcg cccccttgag cgtcgcgccg agatcgacaa
     1981 ggaaggccgc gtcgagactc atcgtgttcg ggtcgttgat gagaccgatc atgcgcaggt
     2041 tgaagaaggc atcccactct gacggcatcg cgtcaagaaa ggtcgcg
//
LOCUS       DAOQUE010000493         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_67868_length_2083_cov_4.405819, whole genome shotgun sequence.
ACCESSION   DAOQUE010000493 DAOQUE010000000
VERSION     DAOQUE010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67868_length_2083_cov_4.405819"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKH10_12225"
     CDS             <1..181
                     /locus_tag="PKH10_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28929.1"
                     /translation="EIREVPGIDLMLKEGARLGLKRIYVPVLRHKPKVKGVDIIELKN
                     IFGLIEEIRTMRIFE"
     gene            241..1740
                     /locus_tag="PKH10_12230"
     CDS             241..1740
                     /locus_tag="PKH10_12230"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNT28930.1"
                     /translation="MRAAVAEALKIAEFSCTIAKKSLDARRKNDIRWQVRVGVSSPEL
                     DGGEMPVAPSFDIPRRKRDGQVAVVGGGPAGLFAALVLARSGFAVTLLEQGPEVAARA
                     RDIARFEQGGAFSPFSNYAFGEGGAGAFSDGKLTSRTKTITKEKAFIFETLIAAGAPE
                     EIRYLAAPHLGSDNLLRILPRLRALLRDEGVTLRFGTTVTDLVVADGRVTALSFSGPE
                     SGTLEVVAALFAPGHSSYRTFRMLLRHGVPFRTKPFAVGCRAEHPQELINEAQWGVKR
                     LDGVKAAEYKLTSDATGLLPVYSFCMCPGGTVVPATPQEGLSVVNGMSRYQRDGRFAN
                     AAIVAAVHPDELFGLPDIAPGAALDELEALERRWYAATGSYAVPAMTIADLLAGKSGG
                     ALSASSYPLGVVPYDISMLLPERVIASLRSGLARFARKLKGYETGVLLGIESKTSCPV
                     QALRDKEGSAENLKGLYVCGEGSGWSGGIVSSAADGIRTALGLLGARGQ"
     gene            complement(1711..>2083)
                     /locus_tag="PKH10_12235"
     CDS             complement(1711..>2083)
                     /locus_tag="PKH10_12235"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT28931.1"
                     /translation="PSRPDLADPNDDEAFSLVGMRHIVRHYEHLLAWIVDEPEQVSLE
                     HYDEEELRDIIIYAARTGRTEALDLLIERTGFDVTVPDAQGLTLLDHAQRDKQYEVVH
                     YLINRGAGHDTELLPSRTEEA"
BASE COUNT          367 a          626 c          701 g          389 t
ORIGIN      
        1 cgagatccgc gaggtgccgg ggatcgacct gatgctgaaa gagggggcgc ggctcggcct
       61 caagcggatc tatgtgccgg tcctgcgcca caagcccaag gtgaaagggg tcgacatcat
      121 agaactcaaa aacatcttcg gactcatcga agagatccgc accatgagga tattcgagta
      181 gcacctatgg gctatcgtga actagacctg cgcctcagac cggggcacac cgatgccgat
      241 ttgcgcgctg cggtggcaga agcgctcaag atcgccgaat tctcctgcac gatagctaaa
      301 aaaagtctcg atgcgcggcg caagaacgat atccgctggc aggtgcgggt cggagtgagt
      361 tcgcccgaac tcgacggcgg cgagatgccg gtcgcgccgt ctttcgatat cccccgccgt
      421 aagcgtgacg gtcaggtcgc ggtggtgggg ggcggtccgg cgggactgtt cgcggcgctc
      481 gtactcgcgc ggagcggttt tgccgtgacg ctcctcgagc agggaccgga ggtcgcggcg
      541 cgggcccgtg acattgcccg gttcgagcag ggaggcgcct tttctccctt ttccaactac
      601 gcgttcggtg aagggggcgc cggtgctttc tccgacggta aattgacctc tcgcaccaaa
      661 acgataacga aggaaaaggc attcatcttc gagacgctca tcgcggcggg cgcccccgag
      721 gagatccgct atctcgccgc accgcacctc gggagcgaca atctgctccg tatcctgccg
      781 cgactccgcg cactgttgcg ggacgaaggg gtgacgctgc ggttcggcac gacggtgacc
      841 gacctcgtcg tcgcggacgg tcgagtgacg gcgctttcgt tcagcggccc cgaaagcggg
      901 acgctcgagg tcgtcgccgc ccttttcgcg ccgggtcatt catcgtatcg caccttccgg
      961 atgctcctgc gacacggcgt tcccttccgt acaaaaccgt tcgccgtcgg ctgtcgcgcc
     1021 gaacacccgc aggaactcat caacgaggcg cagtgggggg tgaaacggct cgacggggtg
     1081 aaggcggccg aatacaaact gacgagcgac gcgacgggtc tgttgccggt ctattcgttc
     1141 tgcatgtgtc ccggcggcac ggtcgtcccg gcgacgccgc aggaagggct gtcggtcgtc
     1201 aacggaatgt cgcgctatca gcgcgacggt cggttcgcca acgcggctat cgtcgcggcg
     1261 gtccatcccg acgaactctt cggcctgccc gacatcgcgc ccggtgcggc gctggacgaa
     1321 ctcgaggcgc ttgaacggcg ctggtacgcg gcgaccggtt cgtatgccgt tccggcgatg
     1381 acgatagccg atcttcttgc cgggaaaagc ggcggtgcgt tatctgcgtc gagttacccg
     1441 ctcggcgtcg tgccgtacga tatctcgatg cttctgccgg agcgagtgat cgcctcactg
     1501 agaagcgggc tcgccaggtt cgcgaggaaa ttgaaggggt atgaaacggg agtgcttctc
     1561 ggtatcgaga gcaagacatc gtgcccggta caagcgctcc gcgacaagga aggcagcgcg
     1621 gagaacctca agggccttta cgtctgcggg gaaggaagcg gttggtcggg tggcatcgtc
     1681 agctcggccg ccgacgggat acggacggcg ctaggcctcc tcggtgcgcg agggcagtag
     1741 ttcggtatcg tgtcctgcgc cccgattgat gagatagtgt accacctcgt attgcttgtc
     1801 gcgctgggcg tggtcgagta gcgtcagccc ctgcgcgtcg ggaacggtca catcgaagcc
     1861 ggtccgctct atgagcagat cgagcgcttc ggtccgtccg gtgcgcgccg catagatgat
     1921 gatgtcgcgc agttcctctt cgtcatagtg ttcgagcgat acctgctccg gttcgtcgac
     1981 gatccacgcg agcaggtgct cgtaatgccg gacgatgtgg cgcatgccga cgagactgaa
     2041 cgcctcatca tcgttcgggt cggcgagatc cggtctcgac ggg
//
LOCUS       DAOQUE010000494         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_67930_length_2082_cov_3.186976, whole genome shotgun sequence.
ACCESSION   DAOQUE010000494 DAOQUE010000000
VERSION     DAOQUE010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67930_length_2082_cov_3.186976"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PKH10_12240"
     CDS             complement(<1..658)
                     /locus_tag="PKH10_12240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT28932.1"
                     /translation="MHDISPIIPVIRRLLEADEFDFLFSRTIEIAADLFTDHPSAVVT
                     RRDDRYWVQVAYPARKTLHFPIAIPGDLSFIPMDGACHRPDIALPEMGTDRFFFFPIG
                     HQDDEKIWGEAWLCLSTSLENDPLLAVQADLFADIVREAVRKIAKIERIKQLTIIDEV
                     TGLYNTRHLFSLLDQAIQQAGRYFSEFSLIFFDLDHFKLVNDSHGHIVGSRLLREIGQ
                     V"
     gene            complement(651..1019)
                     /locus_tag="PKH10_12245"
     CDS             complement(651..1019)
                     /locus_tag="PKH10_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28933.1"
                     /translation="MSKKLRRTLGTSPFKWGVIGVVTSAIFLILSVIFHDSAERRLEE
                     ISGDPRYEVSVWEQLPAEQQQRLGRDLMSARNNLSQIAMGKVIFSITLTISLILLIVS
                     FVRVKNETEQSDDEEHDDDA"
     gene            complement(1016..>2082)
                     /locus_tag="PKH10_12250"
     CDS             complement(1016..>2082)
                     /locus_tag="PKH10_12250"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HNT28934.1"
                     /translation="GLYGDYTLDRQRWDDPDSPRTYQSKYFHVIGLYADAYRLIPRTT
                     VYLGGTILPDGADHWTREAIWGDTRKRTDLTLANALYFSIINWHDISKSRLTITVEGK
                     RYERYWLNYRRMEDLDFSRRYFKPPALVWDNLPLLNDLNTMGLRTRVQFTDRDLTGLT
                     AAAEFIGGLSNPYRDHWEYEEDLSLKEDYWFAGGSLERRFDPVTVSAKAGYLKVNGPS
                     NDDYYGETLYTQLIAGLGIAGFSAKLNLELYRRDLTLHKTPEEQGAIEQRHILDLSWK
                     NMLFASYVGTYYRNKFAQGFVEEDIDEYYPGGSIGFSYRDIRLSVFGGLMRGGLTCIG
                     GVCRYLPDFKGVKMELEVKL"
BASE COUNT          401 a          565 c          679 g          437 t
ORIGIN      
        1 tcacctggcc gatctcgcgc aggagtcgcg acccgacgat gtggccgtgc gagtcgttga
       61 ccagtttgaa gtggtcgaga tcgaagaaga tgagcgagaa ttcagagaaa tagcgtcccg
      121 cctgctggat cgcctgatcg aggagcgaga agaggtgccg cgtattgtag agtcccgtca
      181 cctcgtcgat gatggtgagt tgcttgatgc gctctatctt tgctatcttg cggaccgctt
      241 cgcgcacgat gtcggcgaac aggtcggcct gcaccgcgag gagcgggtcg ttctccagcg
      301 aggtggaaag gcagagccag gcctcgcccc atatcttctc gtcgtcctga tggccgatgg
      361 ggaaaaagaa gaaccgatcc gtccccattt cgggcagagc gatgtcgggg cggtggcagg
      421 cgccgtccat cggtatgaag gaaaggtcgc cgggtatcgc gatcgggaaa tggagcgtct
      481 tgcgcgccgg gtaggcgacc tgcacccagt agcgatcatc gcgtcgggtc acgacggcgg
      541 aaggatgatc ggtgaacagg tcggccgcta tctcgatggt gcgcgagaag aggaaatcga
      601 actcgtcggc ctcgagaagc cgccggatga ccggtatgat gggagagatg tcatgcatcg
      661 tcgtcgtgtt cctcgtcgtc gctttgttcg gtttcgttct tcacccgtac gaacgacact
      721 ataagcagga tgagcgaaat agtcaaagtt atcgaaaaga tgaccttgcc catggctatc
      781 tgcgaaaggt tgttccgtgc gctcatgaga tcgcggccga gccgttgctg ctgttcggcc
      841 gggagttgtt cccagacgct cacctcgtag cgtggatccc ccgatatctc ctcgagacgg
      901 cgttcggcgc tgtcgtggaa gatgaccgac aggatcagga atatcgcgct ggtcacgacg
      961 ccgatgacgc cccatttgaa ggggctcgtc cccagcgttc ttcgtaactt cttgctcata
     1021 gtttcacctc aagttccatt ttgacgccct tgaaatcggg cagataccgg caaacgccgc
     1081 cgatgcaggt gagcccgccg cgcatcagtc cgccgaatac cgacaggcgg atatcacgat
     1141 aggagaatcc gatagagccc cccggatagt attcgtcgat atcctcctcc acaaaacctt
     1201 gggcgaactt gttgcggtag taggtgccga cataggaggc gaagagcata ttcttccaag
     1261 acaggtcgag gatgtgccgc tgttctatcg cgccctgttc ctcaggggtc ttgtggagcg
     1321 tcaggtcccg ccggtacagt tcgagattga gtttggccga gaagccggcg atgcccagac
     1381 cggcgatcaa ctgggtatag agcgtctcgc cgtagtaatc gtcgttcgag gggccgttca
     1441 ccttcaggta gcccgccttg gcgcttacgg tcaccgggtc gaagcgccgc tccagagaac
     1501 cgccggcgaa ccagtaatcc tctttgagcg acagatcctc ctcgtattcc cagtggtcgc
     1561 ggtacgggtt cgagaggccg ccgatgaatt cggccgccgc ggtgagcccg gtcaggtccc
     1621 tgtcggtgaa ctggacccgg gtccggagcc ccatggtgtt gaggtcgttg aggagcggca
     1681 gattatccca aacgagggcg ggcggcttga aataacggcg ggagaaatcg agatcctcca
     1741 tgcgccggta gttgagccag tagcgttcgt agcgttttcc ttccaccgtt atggtcagtc
     1801 ggctcttgct gatgtcgtgc cagttgatga tggagaaata gagcgcgttg gcgagcgtca
     1861 ggtcggtccg tttgcgggtg tcgccccata tcgcctcgcg tgtccagtgg tcggcgccgt
     1921 cgggcaggat ggtgccgccc agatagacgg tggtgcgcgg tatcaggcgg taggcatcgg
     1981 cgtagagtcc gatgacatgg aaatatttgc tctggtaggt gcgcggcgaa tcgggatcgt
     2041 cccagcgctg gcggtcaagg gtgtagtcgc cgtagaggcc gc
//
LOCUS       DAOQUE010000495         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_68081_length_2079_cov_4.075593, whole genome shotgun sequence.
ACCESSION   DAOQUE010000495 DAOQUE010000000
VERSION     DAOQUE010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68081_length_2079_cov_4.075593"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PKH10_12255"
     CDS             <1..194
                     /locus_tag="PKH10_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28935.1"
                     /translation="VTGVNLRAERARRIEQRIALQISPFTELAAELQGAVYAGGRSGD
                     IAPLRERAATLLRSARRMT"
     gene            191..898
                     /locus_tag="PKH10_12260"
     CDS             191..898
                     /locus_tag="PKH10_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28936.1"
                     /translation="MIRSLTLALILLASGILAADDLFDAYTGGDHAAAADILQDRLAE
                     HPHDPLLHYNLGVVEERRGNRGAAVYHYLQALQATPAFPEARNNLDLLTAELHVTVPK
                     QLTEPSSGLATVAVPFFLFLYLFVFALIRHLFRPGWKLRLALIPLFMFTVLFAALFVM
                     RYRAAGMPVYAVALSAGEMRSGPDAALTAVGNVKEGEVLEIAASSDGWAKTKSFQDNI
                     EGWLPASRIHHLTRRVE"
     gene            895..1593
                     /gene="bamD"
                     /locus_tag="PKH10_12265"
     CDS             895..1593
                     /gene="bamD"
                     /locus_tag="PKH10_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNT28937.1"
                     /translation="MNIKTSCLCLVGAACLAAACASAPVVAGAESEAERLYLVGMAYL
                     EDGSFEDAQEQFLKVISDFSYSKWEPFARIGLADSHFKREEFSAAREIYKLFLQMRPG
                     HELADWAAFQLGNCFFEERPSDFWLLPPSEEKDLSDSVEKAVEHYRLYVRDHPQGKHI
                     EEAKKNLAQAETMLVSRELSVAEFYLKKERCPAVKMRVAYIRGQFEKLFDDGPSPELQ
                     ERLNALIARCPMDD"
     gene            1652..>2079
                     /locus_tag="PKH10_12270"
     CDS             1652..>2079
                     /locus_tag="PKH10_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28938.1"
                     /translation="MRPSMAASLAFFFLFSCAKERTPYTGLDLAGPGEPCGGMVFCES
                     WLVCRDGYCEELTPDIDDDGMNGSDPPDLPDEEERPDADLSRLCDGSIPVTAATLPYF
                     ISCEIITGDLLIEETLLSAVEGLKEIRRIDGSLIIRRNILL"
BASE COUNT          387 a          684 c          618 g          390 t
ORIGIN      
        1 ccgtcaccgg cgtgaaccta cgcgccgaac gggcacgccg catcgaacag cggatcgccc
       61 tgcagatcag ccccttcacc gaactcgccg ccgaactgca gggagcggtg tatgccggcg
      121 gacgatcagg cgacatcgct ccgctccgcg aacgggcggc gacgctcttg cgctccgcgc
      181 ggaggatgac atgataagat cgctgacgct tgcgctcata ctgctggcct ccggcatcct
      241 cgccgccgat gacctcttcg acgcctacac cggcggcgac catgcggcgg cggccgatat
      301 cctgcaagat cgcctggcgg agcacccgca cgacccactc ctgcattaca acctcggcgt
      361 tgtcgaagaa cggcgcggga accggggggc ggcggtctac cactacctgc aggcgctaca
      421 agcaaccccg gcttttccgg aagcacggaa caatctcgat ctgttgaccg ccgaactcca
      481 tgtcaccgtg ccgaaacaac tcaccgagcc gtcgagcggg ctcgccaccg tagccgttcc
      541 cttcttcctc tttctttatc tgttcgtgtt cgcgttgata cgacatctgt tccgcccggg
      601 atggaaactg cgcctcgcgc tcatcccgct tttcatgttc acggtgctgt tcgccgcgct
      661 gttcgtcatg cgttaccgcg cggcggggat gccggtctac gccgtcgcgc tctcggccgg
      721 cgagatgcgc agcggccccg acgcggcgct caccgcggtc ggcaacgtaa aggagggcga
      781 ggtgctcgag atcgccgcct catcggacgg ctgggccaaa acgaagagtt ttcaggacaa
      841 tatcgagggg tggctcccgg catcccgcat acatcacctg acacggaggg tagagtgaac
      901 atcaagacat cgtgcctgtg ccttgtcggc gcggcctgcc tcgccgccgc ctgcgcctcg
      961 gcgccggtcg tcgccggggc cgaatcggaa gcggagcggt tgtacctcgt cgggatggcc
     1021 tatctcgagg acggcagttt cgaagatgcg caggaacagt tcctcaaggt catctccgac
     1081 ttcagttaca gcaagtggga gccgttcgcc cgcatcgggc tggccgattc gcacttcaaa
     1141 cgcgaggagt tctcggccgc ccgggagata tacaaacttt tcctgcagat gcgaccgggg
     1201 cacgaactcg ccgactgggc cgccttccaa ctcggcaact gtttcttcga ggagcggccg
     1261 agcgatttct ggctcctgcc gccgtcggag gaaaaggacc tgtcggacag cgtggaaaag
     1321 gcggtcgaac actaccgtct ctatgttcgc gaccacccgc agggcaaaca tatcgaagag
     1381 gcgaagaaaa acctcgcgca ggccgaaacg atgctggtat cgcgggaact cagcgtggcc
     1441 gaattctatc tcaagaagga gcgctgtccc gcggtgaaga tgcgggtcgc ctatatccgc
     1501 ggacagttcg agaaactctt tgatgatggc ccctcccccg aactgcagga gcggctcaat
     1561 gcgcttatag cccgctgtcc gatggacgac tgaccacccc ggttgactct tttccctgtt
     1621 tcggatacga tccggaccgg aggtgttccc catgcgtccc tctatggccg cgtcactcgc
     1681 cttctttttt cttttttcct gcgccaagga acgcaccccg tacaccggat tggatctcgc
     1741 cggtcccgga gaaccttgcg gcggcatggt gttctgtgaa tcgtggctgg tatgccgtga
     1801 cgggtactgt gaagaactga cgcccgacat cgatgatgac gggatgaacg gatcggaccc
     1861 gccggacctg ccggacgagg aggagaggcc cgacgccgac ctttcacgcc tctgcgacgg
     1921 ctccatcccg gtaacggcgg caacgctccc ctatttcatc tcatgcgaga tcatcaccgg
     1981 cgatctcctc atcgaggaga ccctgctcag cgcggtcgaa ggactcaaag agatacgccg
     2041 tatcgacggc tcactcatca tccggcgcaa catcctgct
//
LOCUS       DAOQUE010000496         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_68950_length_2063_cov_4.734064, whole genome shotgun sequence.
ACCESSION   DAOQUE010000496 DAOQUE010000000
VERSION     DAOQUE010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68950_length_2063_cov_4.734064"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1372
                     /locus_tag="PKH10_12275"
     CDS             104..1372
                     /locus_tag="PKH10_12275"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT28939.1"
                     /translation="MSDSSVSSLLRDRKFRILYAALLISGIGAGISRISLLWLAYHLT
                     GSTVTMALVFICFTLPGLLVSPISGAIADKYPKRDLFIGASVVIGVVVLLFPIAYHTG
                     SLTILYGAVVLKGIALAFKDGPMRAYLPELFAEEQLPAVNAMLENNRSLVIILGPAIG
                     GFMLGFGYVDQAFILDSLSFMTAAGLFLLLPRTRNALSEGTLELSRIMGDIREGMKII
                     AASPIHRFLLLLFVVLNGFSCMIGGLYMPFSEDILSPANGLSGGTIFAMLETTVGVGA
                     FVGTLFISRIMEKAGPLRTLMLGSTLLAFEILLYGLVPNIFVLIPVTLVTANAIPLLL
                     VPFFTLLQQKTAPDFRGRVMGATESILLTIVSLAFGLGGVLAQVVGILPLFITSGVLL
                     IASMVVIMLLPLYRALRTEEQQELQPQGVT"
     gene            1369..>2063
                     /locus_tag="PKH10_12280"
     CDS             1369..>2063
                     /locus_tag="PKH10_12280"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNT28940.1"
                     /translation="MKRALMVAAVMVVSAVLWGEEPPAPQAVEQEKEDVTPLLREEKV
                     EEKKEEQKEKPVAEEIFSTNERKKKGEKEKKLFFRGDIGFTWVSGNGSSYKNDTLAAY
                     FNMSTTWNDNIAKIILKYDQKILPRLSDQNQSQAEIYQTNRIDLNFDYYLANRWEFFS
                     FTEMESDPTKKLTYRFGVASGIKFVLFRNQYWLFDLGVAPKFEFTTYVDGPQRLKEFR
                     SSSRVKMVFTLHE"
BASE COUNT          469 a          520 c          546 g          528 t
ORIGIN      
        1 tcatcggtcg ttcggaagaa ctggccgacg agatgaagtc gagccgtttc cttgaggact
       61 cgatgcgttc gaccatagat tcgcttttca agtggagacg ctgatgagtg actcatcggt
      121 ctcctccctg ttgcgcgacc ggaagttccg gatactctac gcggcgctcc tcatatcggg
      181 catcggtgcc ggcatcagcc ggatatcgct tctgtggctc gcctatcacc tgaccggttc
      241 gaccgtgacg atggcgctcg tcttcatctg tttcaccctg ccgggtttgc tggtgtcgcc
      301 catttcgggg gccatcgccg acaaataccc caagcgggat ctgttcatcg gcgcctcggt
      361 cgtcatcggc gtcgtcgtgc tccttttccc gatcgcctat cataccggca gcctgacgat
      421 actgtacggc gctgtcgtgc tcaagggcat tgctcttgcc ttcaaagacg gaccgatgcg
      481 cgcctatctg cctgaactct tcgccgagga acagttgccg gcggtcaacg ccatgctgga
      541 gaacaatcga tcgctcgtta tcatactggg tccggccatc ggcggcttca tgttgggttt
      601 cggttatgtc gatcaggcgt tcatccttga ttcgctgtcg ttcatgacgg ccgccggcct
      661 gttcctgctc ctgccgcgga cacgcaacgc gctcagcgaa ggaacgctgg aactttcgcg
      721 tatcatggga gatatcagag agggcatgaa gatcatcgcc gcctcgccga tacatcgctt
      781 cctgctcttg cttttcgtcg ttctgaacgg cttttcctgc atgatcggcg ggctctacat
      841 gccattcagc gaggatatcc tctcgccagc caacggccta tcaggcggta ccattttcgc
      901 gatgctggag accaccgtgg gggtcggtgc ctttgtcggt actcttttta tctcacgcat
      961 catggagaag gccggtccgt tacggaccct gatgctgggg agtaccctac tcgcattcga
     1021 aatactttta tacggtctgg tgccgaacat cttcgtgctc attcccgtca cactggtgac
     1081 ggcgaacgct atccccctcc tgctggtgcc gttctttacc ttgttacaac agaagacagc
     1141 gcccgatttc cgggggcggg tcatgggagc caccgaatcg atattgctca ccatcgtgtc
     1201 gcttgccttc gggctgggcg gtgtattggc gcaggtggtg gggattttgc cgctgttcat
     1261 aacgagcggt gtgcttctga tagcgagtat ggtcgtcatt atgttgttac cgttatatcg
     1321 tgccttacgg accgaagagc aacaggaact gcaaccacag ggggtgacat gaaaagagca
     1381 cttatggtcg ccgccgtgat ggtggtcagc gccgtactgt ggggagagga gccgcccgct
     1441 ccgcaggccg tagaacagga gaaagaggat gtgacgcccc tgttaagaga agaaaaagtt
     1501 gaagaaaaaa aagaggaaca gaaagaaaag cctgttgctg aagagatctt ctcaaccaat
     1561 gaaagaaaaa agaaaggaga aaaagaaaaa aaactttttt tcagaggaga cataggattt
     1621 acatgggtgt caggaaatgg ttcttcttat aagaatgaca cattggctgc ctattttaat
     1681 atgtctacta catggaatga taatatagcc aagatcatac tgaaatatga tcagaaaata
     1741 cttcctaggt tatctgatca gaatcaatca caagctgaga tatatcaaac caacagaatt
     1801 gacttaaact ttgactatta tcttgccaac aggtgggagt tcttttcctt tacggagatg
     1861 gaaagtgacc cgacaaaaaa gttaacatat aggttcgggg ttgcatccgg cataaaattc
     1921 gttttgttta ggaatcaata ttggcttttt gacttgggtg tcgccccgaa attcgaattt
     1981 actacatatg ttgacggccc ccagcgtctt aaagaatttc gctcgtctag cagagtaaag
     2041 atggtcttta ctctccatga gtt
//
LOCUS       DAOQUE010000497         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_68960_length_2063_cov_3.695717, whole genome shotgun sequence.
ACCESSION   DAOQUE010000497 DAOQUE010000000
VERSION     DAOQUE010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68960_length_2063_cov_3.695717"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PKH10_12285"
     CDS             complement(<1..603)
                     /locus_tag="PKH10_12285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT28941.1"
                     /translation="MFAAVKNYFSKFKIMKTVSKDFWLVNAIQFFDGLAYFAVIIVIS
                     LYLTDNVGFSDMEAGAWQGIFFLFITAFMFSVGSICDTLGVKKTFFIAALLLMVSRLG
                     MGIAPQFLTGDPLKYLVMALLLVMALGTATLVTNTNAALRHFTTPENRGTGFNVYYLI
                     MNIGAAIAGFGVTDGFRSVFGPVNGNLMILNFSFAMAVICF"
     gene            748..2052
                     /locus_tag="PKH10_12290"
     CDS             748..2052
                     /locus_tag="PKH10_12290"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNT28942.1"
                     /translation="MYPVYDLIADKRDGKELHEDALLWLIDAYLRDEVTDYQMAAFLM
                     AVCIRGFSPAELSFWTDRMLHSGEVLSFPADGRPVVDKHSTGGVGDKISIPLAPLLAA
                     LGFRVPMISGRGLAHTGGTLDKLESIPGFRTDLSLDRFKQQVQELGVAMIGQTAEIAP
                     LDRRLYALRDVTGTVESVPLIASSIMSKKLAEGIDGLLLDVKTGDGAFMREMHDAKRL
                     ARTMKDIGERAGTKVTVLLTDMSESLGRTAGHALEIAESLAVLRGEKVPQVSDLVERQ
                     AAEILMLFGKAATRAAAVGRAREKLADGTALSIFRRMVEAQGGDPRVIDSPALLPAAP
                     HIHELRAARGGFVAQVAARACGRAVVALGGGRQRKEDALDPAVGLTFPKKVGDRVTKG
                     EPVIRLHYRDRSRLDAALDLLEGAVAVTATPPATPRLIRGRL"
BASE COUNT          407 a          639 c          686 g          331 t
ORIGIN      
        1 aaagcagatg accgccatcg cgaaactgaa gttgaggatc atcaggttgc cgttcaccgg
       61 tccgaaaaca ctccggaacc cgtcggtcac gccgaacccg gcgatggcgg cgccgatgtt
      121 catgatgagg tagtagacat tgaacccggt gccgcggttc tcgggcgtcg tgaagtgacg
      181 cagggcggcg ttggtgttgg tcaccagtgt cgcggtgccg agcgccatca cgagcaggag
      241 cgccatgacg aggtatttca gcgggtcgcc cgtcaggaac tgcggcgcga tccccatccc
      301 gagccgggac accatgagca ggagcgccgc gatgaagaag gtcttcttca ccccgagcgt
      361 gtcgcagatg gagccgaccg agaacatgaa cgcggtgatg aacaggaaga atatcccctg
      421 ccacgccccc gcctccatgt cgctgaagcc gacattgtcg gtgagataca gtgagatgac
      481 gatgatgacc gcgaaatagg cgagcccgtc gaagaactgg atggcgttga ccagccagaa
      541 gtccttcgac accgtcttca tgatcttgaa tttggagaag taattcttta ccgcggcgaa
      601 catgtacctg cctccattga gaatagacag gtaatcgtaa cgggacacac cccaaagtca
      661 aggaaaccgc ccgttaccta aagatttttt gacttcaccg atggcctttc atagaatggg
      721 ccgttcatca agagccggag aaccgtgatg taccctgtgt acgacctcat agcggacaaa
      781 cgcgacggga aggaacttca cgaggacgcc ctgctctggc tcatcgacgc ctatctgcgc
      841 gacgaggtga ccgactacca gatggccgcc ttcctcatgg cggtctgcat ccggggcttc
      901 tcccccgccg aactgtcgtt ctggaccgac cggatgctcc attcgggcga ggtgctttcg
      961 ttccccgccg acggccgccc ggtcgtggac aagcactcga ccggcggggt gggcgacaag
     1021 atatcgatac cgctcgcgcc gcttctcgcc gcgctcggct tccgcgtgcc gatgatctcg
     1081 ggccgggggc tcgcccatac cggcgggacg ctcgacaaac tcgaatcgat accggggttc
     1141 cgcaccgacc tgtcgctcga caggttcaaa caacaggtgc aggaactggg cgtcgcgatg
     1201 atcgggcaga ccgccgagat agcgccgctc gaccggcggc tctacgccct gcgcgatgtg
     1261 accgggacgg tcgaatcggt gccgctcatc gcgagttcca tcatgtcgaa gaaactggcc
     1321 gaagggatag acgggctcct gctcgatgtg aagacaggcg acggcgcctt catgcgcgag
     1381 atgcacgacg cgaagagact cgcccggacg atgaaggata tcggcgagcg ggcgggaacg
     1441 aaggtgacgg tgctcctcac cgacatgtcc gagtcgctcg gccggacggc gggccacgcg
     1501 ctcgagatcg ccgaatcgct ggcggtcctg cgcggcgaga aggtgccgca ggtgagcgac
     1561 cttgtggagc gacaggcggc tgaaatcctt atgcttttcg gtaaagcggc gacgcgcgcc
     1621 gcggcggtcg gtagggcgcg ggaaaaactc gccgacggca ccgcactctc gatattccgg
     1681 cggatggtcg aggcgcaggg aggcgacccg cgcgtcatcg acagccccgc gctcctgccg
     1741 gcggcgccgc acatccacga actgagagcg gcacgcggcg ggttcgtggc gcaggtcgcg
     1801 gcccgggcct gcggccgggc ggtcgtggcg ctcggcgggg gacgccagcg caaggaggac
     1861 gcgctcgacc cggcggtcgg cctgaccttc ccgaaaaagg tcggcgaccg ggtcacgaag
     1921 ggagagccgg tcatccgact gcattaccgc gaccggtcac gactcgacgc cgcactcgac
     1981 ctgctggagg gggcggtggc cgtgaccgcg acgccgccgg cgacgccacg gctcatccgg
     2041 ggacggctgt aatttgagcg tac
//
LOCUS       DAOQUE010000498         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_69700_length_2049_cov_4.778335, whole genome shotgun sequence.
ACCESSION   DAOQUE010000498 DAOQUE010000000
VERSION     DAOQUE010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69700_length_2049_cov_4.778335"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PKH10_12295"
     CDS             complement(<1..800)
                     /locus_tag="PKH10_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT28943.1"
                     /translation="MLTKGLLLKLFDAAHMQRWNDRLRPVALFELDKQAHKMIAAVHF
                     ARFEKEKRPVDLNLLIEGALFEMLQRVVLTDIKPPVFHRIKEDAAKYRQLNDWIYEQL
                     KKYLLPLGDGTADRFRAYFTEPEDTVERRILGAAHLFATRWEFGLIERLNTGYFDVED
                     IRRSLDERLAAYGDLAGMEEIAKHGGNNNSRHFLDLCGQLRFQYRWSNLHMMPRISVL
                     GHMLLVGFVAYLHSREIGACRRRAVNNFLTGLYHDLPEVLTRDIISPV"
     gene            complement(805..>2049)
                     /locus_tag="PKH10_12300"
     CDS             complement(805..>2049)
                     /locus_tag="PKH10_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28944.1"
                     /translation="PGTGGTLSFKTSPAGVEVVLDPDTSAERTCISPCSFEQVPLGDH
                     YVDFRKSPYKTGGYAIRVEGGVRRIEENFQIDAVKAPQAAIDMALQGDYDGVRDLLSQ
                     NALIINTRRGNLTLFWILLDKAPQETLRSLFAVAAKNSSIARGAYCTLDVYQKALEKG
                     YSDLARQCCYLFPNAGEVAKRTPAGQEATGAYCTEQIEKRTTTTKNIQNYFLMAGLTT
                     PLVGTEIDVYRAAISKHFLLGAFTFRLGLDVPGNVYGGVSTLWIGGRYLWGEKKNYEI
                     GIISSISPFFSGTSYFSHLYESQVYNGYEYITYNTAVTRASWFYTRAFFAYHFDSFFL
                     EAGVAFPLLWYQTTIANKDAVGEYSSNYVTKTKMDGQIALDILLPFDLTGGASSTGST
                     KTTYSYYCKALPPMFFYFGIGL"
BASE COUNT          389 a          547 c          695 g          418 t
ORIGIN      
        1 ttgaccggtg atatgatgtc gcgggtgagc acctcgggca ggtcgtggta gagcccggtg
       61 aggaagttgt tgacggcgcg gcgacggcac gcgccgatct cgcgcgaatg aaggtaggcg
      121 acaaagccca ccagcagcat gtggccgagc accgatatgc gcggcatcat gtgaaggttc
      181 gaccagcggt actggaaccg cagttgtccg cagaggtcga ggaagtggcg gctgttgttg
      241 ttcccgccgt gcttggctat ctcctccatc ccggccagat cgccgtaggc ggcgagccgt
      301 tcgtcgagcg agcggcgaat gtcttctaca tcgaaatagc cggtgttgag ccgctcgatg
      361 agcccgaact cccagcgggt cgcgaagagg tgcgccgcgc cgaggatgcg ccgctcgacg
      421 gtatcctccg gctcggtgaa gtaggcgcgg aaccggtcgg cggtgccgtc gccgagcggc
      481 aggaggtatt ttttcagttg ttcgtagatc cagtcgttga gttgccggta tttcgccgca
      541 tcctccttga tgcggtgaaa gacgggcggt ttgatgtcgg tcaggacgac gcgttgaagc
      601 atctcgaaga gtgccccttc gataaggaga ttcagatcga ccggccgttt ttccttctcg
      661 aatcgggcga agtggaccgc cgcgatcatc ttgtgcgcct gtttgtcgag ttcgaacagc
      721 gccaccggcc gcagacggtc gttccaccgc tgcatgtggg cggcgtcgaa cagtttgagg
      781 agcagtccct tggtcaacat ggcgtcagag gccgatgccg aaatagaaga acataggcgg
      841 cagggccttg cagtaatagg agtaggtggt cttggtgctt ccggtcgaag aggcgccgcc
      901 ggtgagatcg aacggcagga gtatgtcgag ggcgatctgc ccgtccattt tcgtcttggt
      961 gacatagttc gaactgtatt cgcccaccgc gtccttgttc gctatcgtcg tctggtacca
     1021 gaggagcggg aaggcgacgc ccgcttcgag gaagaacgaa tcgaaatggt aggcgaaaaa
     1081 ggcgcgggtg tagaaccatg aggcgcgggt caccgcggtg ttgtaggtga tatactcata
     1141 gccgttgtag acttgggact catagaggtg tgagaagtaa ctggtgccgg agaagaaggg
     1201 ggaaatggaa ctgatgatgc cgatctcata gttctttttt tcgccccaca gataccgccc
     1261 gccgatccag agggtgctca cgccgccgta cacattcccg gggacatcga ggccgagccg
     1321 gaaggtgaag gcgccgagaa ggaaatgctt cgatatggcg gcgcgataca catctatctc
     1381 ggtgccgacc agcggcgtgg tgagccccgc cattagaaag taattctgta tgttcttggt
     1441 ggtggtcgtg cgcttttcga tctgttcggt gcagtaggcg ccggtcgcct cctgtcccgc
     1501 cggggtccgt ttcgcgacct cgccggcgtt gggaaacagg taacagcact gccgtgcaag
     1561 gtcggagtat cctttttcaa gcgccttctg gtacacatcg agcgtgcagt aagcgccgcg
     1621 cgcgatggag gagttcttcg ccgcgacggc aaagagcgaa cggagcgtct cctgcggcgc
     1681 cttatcgagc agtatccaga agagcgtcag gttgccgcgc cgggtgttga tgatgagcgc
     1741 gttctgcgag agcaggtcgc ggacgccgtc atagtcgccc tgcagggcca tgtcgatggc
     1801 cgcctgcgga gccttcaccg catctatctg gaaattctcc tcaatgcggc ggacgccgcc
     1861 ttcgacgcgg atcgcatagc cgccggtctt gtagggtgac ttgcgaaaat cgacatagtg
     1921 atcgccgaga ggtacctgtt cgaaagagca gggggatatg caggtccgtt ccgccgaggt
     1981 gtcggggtcg agcactacct cgacgccggc cggtgaggtc ttgaaagaga gcgtgccgcc
     2041 ggtgccggg
//
LOCUS       DAOQUE010000499         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_70602_length_2032_cov_3.646940, whole genome shotgun sequence.
ACCESSION   DAOQUE010000499 DAOQUE010000000
VERSION     DAOQUE010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70602_length_2032_cov_3.646940"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="PKH10_12305"
     CDS             complement(<1..1480)
                     /locus_tag="PKH10_12305"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNT28945.1"
                     /translation="MKRPLSFLFVLLIGTAALWGASKQPLPAWETDAEKAAKETRVPF
                     DLRLRIPPQNVPYGPAEFAAMEGALIQVQTYNTAIRKYYGDMINGIVAAQAIPYIIVE
                     NTTERNNVVSQVLNAYGIAESDVTFLTYPYDANWTRDYGPWHIYPDKGTRALVDHEYY
                     DGRPNDDAIPPKLGALWGDEVFVSGFYTEGGNFMTDGRGTCWMSTGVFDKNNLADNPT
                     NRATVAQLFKEYVGCDSVHFPVSIPGEGTTHIDMYSKILDQDTIIVSYSTSDWGADAD
                     ELAQLDAAAELYANTPKPGGGKFTVVRIPMTFGGSFFSTTYYTHTNSLIVNDHVLVPI
                     YGRGTDEEALQIYRDLMPHHTIVGITSNEIIPQGGSIHCTTMQIPVKQYKACGDGVIG
                     DGEECEPLYWGGVTDCTDLGYESGDIFCDENCQFDVSQCVALVTCGNGTIDSGELCDG
                     GTIDCTAIAGKDYIQGTATCLPSCGAWDESDCESDTPDDTPQP"
     gene            complement(1586..>2032)
                     /locus_tag="PKH10_12310"
     CDS             complement(1586..>2032)
                     /locus_tag="PKH10_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28946.1"
                     /translation="NDAWNDEFGWHNDIDLPPVRDIFFVAIETDYAAWEEIQGTPDAI
                     AEMNPFLKRVADLYLAGRLKIMIIAFVTGVVTAVLTFPFAFPRRSPFRRLDLSAAATG
                     WGLFGLLSFGVAFAVPPLFLPTYLLTLAVGISFALLLLVLARKLEK"
BASE COUNT          358 a          571 c          697 g          406 t
ORIGIN      
        1 cgggctgggg ggtgtcgtcg ggggtgtcgc tctcgcaatc cgactcatcc cacgcgccgc
       61 aactgggtag gcaggtcgcc gtgccttgga tgtagtcctt cccggcgatg gcggtgcagt
      121 cgatggtgcc gccgtcgcag agctcgccgc tgtcgatcgt gccgttgccg caggtcacca
      181 gcgccacgca ctgcgacaca tcgaattggc agttctcgtc gcagaagatg tcgcccgact
      241 cgtagccgag gtcggtacag tcggtcacgc cgccccagta gagtggttcg cactcctcgc
      301 cgtcgccgat gacgccgtcg ccgcaggcct tgtactgctt gaccggtatc tgcatggtgg
      361 tgcagtggat ggagccgccc tgcgggatga tctcgttgga ggtgatgccg acgatggtgt
      421 ggtgcggcat caggtcgcgg tatatctgga gcgcctcctc atcggtgccg cgcccgtaga
      481 tcgggacgag cacatggtcg ttgacgatga gagagttggt gtgggtgtag taggtggtcg
      541 aaaagaacga accgccgaag gtcatcggga tgcggacgac cgtgaacttc ccgccgcccg
      601 gcttcggggt gttcgcgtac agttcggccg ccgcgtcgag ttgggcgagt tcgtccgcgt
      661 cggcgcccca atcggaggtc gaataggaaa cgatgatggt gtcctgatcg agtatcttcg
      721 agtacatgtc gatgtgggtg gtgccctcgc ccggtatcga cacggggaaa tggaccgaat
      781 cgcagccgac atattccttg aacagttgag cgacggtcgc gcgattggtg gggttgtcgg
      841 cgaggttgtt cttgtcgaac acgccggtgg acatccagca ggtgccgcga ccgtcggtca
      901 tgaagttgcc cccttcggtg tagaacccgc ttacgaacac ctcgtcgccc cagagcgcgc
      961 cgagtttcgg cggtatcgcg tcgtcgttcg ggcggccgtc gtagtattcg tggtcgacca
     1021 gcgcgcgggt ccccttgtcc gggtagatat gccacgggcc gtagtcgcgg gtccagttcg
     1081 cgtcataggg gtaggtgaga aaggtgacat cgctctccgc gatgccgtag gcgttgagca
     1141 cctgcgagac gacattgttc ctctcggtgg tgttctcgac gatgatgtag gggatcgcct
     1201 gagccgcgac gatgccgttg atcatgtcgc cgtagtattt ccggatggcg gtgttgtagg
     1261 tctgtacttg aatgagtgcc ccctccatcg cggcgaactc ggctggaccg taggggacat
     1321 tctgcggcgg gatgcgcagt cgcaggtcga acgggacgcg ggtctccttc gccgcctttt
     1381 cggcgtcggt ctcccacgcc gggagcggct gtttcgacgc gccccagaga gcggcggtgc
     1441 cgatgagcag gacaaaaagg aaggagagcg ggcgtttcat gatgcacctc ttttatgtgt
     1501 ttctcaacgg taggaccgac atgctctttt ttcagcaaaa cggggtcctg ccattttaag
     1561 ccatgtgata acgggatgtt gcgtcttact tctccagttt tcgcgccaga acaagcagca
     1621 ggagggcgaa agagataccc accgcgagcg tcagcagata ggtcggcagg aacagcggcg
     1681 gcaccgcgaa ggcgacgccg aacgaaagaa gaccgaaaag cccccagccc gtggcggcgg
     1741 cggaaagatc gagtctgcgg aacggcgacc ggcgcggaaa ggcgaagggg aaggtcagga
     1801 cggcggtcac gacgcccgtc acgaaggcga tgatcatgat cttgagccgc ccggcgagat
     1861 acaggtcggc gacccgcttg aggaacgggt tcatctcggc gatggcgtcg ggggttccct
     1921 ggatctcctc ccatgccgca tagtcggtct cgatcgcgac gaagaatata tcgcgcaccg
     1981 gcggcaggtc gatgtcgttg tgccaaccga actcgtcatt ccaggcgtcg tt
//
LOCUS       DAOQUE010000500         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_70608_length_2032_cov_3.168943, whole genome shotgun sequence.
ACCESSION   DAOQUE010000500 DAOQUE010000000
VERSION     DAOQUE010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70608_length_2032_cov_3.168943"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKH10_12315"
     CDS             complement(<1..714)
                     /locus_tag="PKH10_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28947.1"
                     /translation="MRNAGRWFIALSVMLISGMLCAKSLSIFPTAWKKAGDRSFAESL
                     DENFLLMLEKVKGVDASGADALSSDIKKQVKKCGGKIACIERAAKAQTDADLAAILAY
                     GGGRIEVTIFNKKGKKVGESAVDVAEDDEPEDVVGGLLGSINKLLSKVQDDTDDGGSS
                     SSSTDEEDSSSASSGPAKSLSASEKKEVRRKGFKAYQGADYKGAVSSFRTADDTELAD
                     MTEDINKLLGKAKDAVQSQD"
     gene            862..937
                     /locus_tag="PKH10_12320"
     tRNA            862..937
                     /locus_tag="PKH10_12320"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:895..897,aa:Ala,seq:cgc)
     gene            complement(945..>2032)
                     /locus_tag="PKH10_12325"
     CDS             complement(945..>2032)
                     /locus_tag="PKH10_12325"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HNT28948.1"
                     /translation="DDSEMSLAHLRNLLSKDGYVVATASGGEEALAFVEQRVPDLILL
                     DIVMPRVSGFDVIRKLKTMPRTAQAPVIFLTSNEDEGSKMTAFSLGAVDYVVKPFNPQ
                     EVRARVRVHLKIAVALQEAVESQADKIRQIQEAQRSLFIRPEDLREARFSVYCRSVVE
                     AGGDFYDIVDPAEGIFSYLVADVSGHDIATSYIHPALKALFRQNSSPLHTLAETATMM
                     NKVLERILPPERYLSAFFLSVNRNAGRVSWIAAGHPPALFIPVEGEPFFLEGEDELIG
                     VNGASLYHMNERPVREGDRIILYTDGLVECGTGTSVWGAEYRALLQFAPGLRDIPIGE
                     VPRLLVERSVPDVEKADDDIIVLCVDV"
BASE COUNT          427 a          638 c          549 g          418 t
ORIGIN      
        1 atcctgcgac tgcaccgcat ctttcgcctt gcccagcaac ttgtttatgt cctcggtcat
       61 gtcggcgagt tccgtgtcgt cggcggtacg gaaagaggag accgcgccct tatagtccgc
      121 accctgatag gccttgaacc ctttacgacg gacctctttc ttctcgctgg ccgaaaggct
      181 cttggccggc ccagaagaag cggaggaaga gtcctcctca tccgtcgagg acgatgacga
      241 gccgccgtcg tcggtatcgt cctgcacctt ggagagcagt ttgttgatgg accccaataa
      301 tccgccgacg acatcctcgg gctcatcgtc ttcagcgaca tcgacggcgc tttcaccgac
      361 cttcttgccc tttttattga agatcgtcac ttctatgcgg ccgccaccgt aggcaaggat
      421 ggccgcgaga tcggcgtcgg tctgcgcctt tgccgcccgt tctatgcagg cgatcttgcc
      481 gccgcacttc ttcacctgtt tctttatgtc gctcgaaagc gcatccgcac ccgaggcatc
      541 gacacccttc accttctcga gcatgagcag gaaattctca tcaaggcttt cggcgaacga
      601 acggtcaccc gcctttttcc atgccgtagg gaagatcgag agcgatttcg cgcaaagcat
      661 gccggaaatg agcatgaccg acagcgcgat gaaccagcga cccgcatttc tcaaggaagc
      721 ctccttctgt tggaagaaca cgcgcgttct caattaaata cgcgccccac catatacaaa
      781 aggcgggatg atgtcaaaaa aaatgacggt cgccaagaat gtttgacaaa tcctcttttg
      841 tcgctataaa aggggccggt cggggctgta gcgcagttgg gagcgcgcta gaatcgcact
      901 ctagaggcca ccggttcgat cccggtcagc tccaccactt ttcttcaaac atccacgcat
      961 agaactatga tgtcgtcatc ggctttttcg acatcaggca ccgagcgttc gacaagaagt
     1021 cgcggcacct cgccgatggg tatgtcccgc agacccggcg cgaactgcag aagtgcccgg
     1081 tattcggccc cccagaccga tgttcccgtc ccgcactcga ccagcccgtc cgtgtacagg
     1141 ataatgcgat ccccctcgcg gaccggtcgt tcgttcatat gatacagcga ggccccgttg
     1201 acgccgatga gttcatcctc cccctcgagg aagaagggtt ctccctcgac cgggatgaag
     1261 agcgccgggg ggtgaccggc cgctatccac gacaccctgc cggcgttacg gttgaccgac
     1321 agaaaaaaag ccgaaagata gcgctcgggt ggcagtatcc tctcaagcac cttattcatc
     1381 atggtcgccg tttcggcgag cgtatggagc ggcgaactgt tctgccggaa caacgccttg
     1441 agcgccgggt gaatgtaact ggtggcgata tcgtgtcccg agacatcggc gaccagatag
     1501 gagaatatac cctccgccgg atcgacgata tcgtaaaaat cccctcccgc ctcgacgacc
     1561 gaccggcagt agaccgaaaa acgcgcctca cgcagatcct ccggccggat aaagagcgac
     1621 cgttgcgctt cctgtatctg ccggatctta tccgcctgag attccaccgc ctcctgcagg
     1681 gcgacggcga tcttcaggtg gacgcgcacc cgggccctga cctcctgcgg attgaacggc
     1741 ttgacgacat aatcgaccgc cccgagcgag aaagcggtca tcttcgaccc ttcgtcctca
     1801 ttgctggtca ggaatatgac cggcgcctgc gcggtacggg gcatcgtctt gagttttcgt
     1861 atcacatcga atccgctcac ccgcggcatg acgatgtcga gaagtatcag gtcaggcacc
     1921 cgctgttcga cgaaggcgag cgcctcttcg ccaccggaag ccgtcgccac gacatagcca
     1981 tccttggaga gcaggttccg caggtgtgca agcgacatct cggaatcatc ga
//
LOCUS       DAOQUE010000501         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_71136_length_2023_cov_3.081809, whole genome shotgun sequence.
ACCESSION   DAOQUE010000501 DAOQUE010000000
VERSION     DAOQUE010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71136_length_2023_cov_3.081809"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="PKH10_12330"
     CDS             complement(<1..1610)
                     /locus_tag="PKH10_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNT28949.1"
                     /translation="MNIGEIKAAHKRVTRWEYKPGRIEKGYNDRTLHIDLTTNTITEK
                     PVTPLMKDKFVGGKGFGLYLLWHATRPQTKWNDPENEVIISPGPCAGITQYAGAGKSL
                     VVSISPQTGGIMDSNVGGYFGPYMKFSGFDALELQGKAQEDVIIVIDGTDNSVTIETA
                     PKEAIDSHVLAEQLTEMYAKDDKDKLNVAVVSAGTAADHSLIGMLNFSFWDPRRKVAR
                     LKQAGRGGIGTVFRDKRIKAVVCKTTGIKPNWNNVVDLNAIVERGQRFQKEIKELDDK
                     QCEMRDKGTAHLMEIMDAYDLLPTTNFRFGKAADIGEDINKIHSNVLKSLFNLKWRDL
                     DGCWFGCAMSCCKSVDGFELKTGPYKGDKVTVDGPEYETAAGLGSNCGIFDPLHIIEA
                     NFYCDTYGICTITVGTITAFVMECYEAGILNKDRTGGLELTFGNSAAALELIHQMARG
                     EGFGAIAGRGAQYMKNYFADQGWGDRQFLNDIAMVNKGLEYSQYMSKESLAQQGGYAM
                     TNKGPQHDEAWLIFMDMVNNQIPSFEDKA"
     gene            complement(1784..>2023)
                     /locus_tag="PKH10_12335"
     CDS             complement(1784..>2023)
                     /locus_tag="PKH10_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT28950.1"
                     /translation="RMDLAHGEFYGVIGGMGGDCAHCNRIRLLANGSVKPCLFSDLGF
                     SVRELGAEEAIRRAIAAKPAKGTCNTQGDFYNIGG"
BASE COUNT          333 a          603 c          623 g          464 t
ORIGIN      
        1 gccttgtcct cgaacgacgg tatctggttg ttgaccatat ccatgaagat gagccaggcc
       61 tcgtcgtgct gcggcccctt gttggtcatc gcgtagccgc cctgctgggc gagcgattcc
      121 ttcgacatgt actgcgagta ttcgagcccc ttgttgacca tcgcgatgtc gttcaggaac
      181 tggcggtcgc cccaaccctg atcggcgaag tagttcttca tgtactgggc gccgcgtccg
      241 gctatcgcgc cgaaaccctc tccgcgcgcc atctggtgga tgagttcgag cgccgccgcc
      301 gagttgccga aggtgagttc gagcccgccg gtccgatctt tattgagtat ccccgcttcg
      361 tagcactcca tcacgaaggc cgtgatcgtg ccgacggtga tggtgcagat gccgtaggtg
      421 tcacagtaga agttcgcctc gatgatgtgc agcggatcga agatgccgca gttggagccg
      481 agcccagccg ccgtctcgta ctcggggccg tcgacggtga ccttatcgcc cttgtagggg
      541 ccggtcttca gttcgaagcc gtcgaccgac ttgcagcacg acatcgcgca gccgaaccag
      601 cagccgtcga ggtcgcgcca cttgaggttg aagaggcttt tgagcacgtt ggaatgtatc
      661 ttgttgatgt cctcaccgat gtcggccgcc ttgccgaacc ggaaattggt ggtcggcagg
      721 agatcgtagg catccatgat ctccatgagg tgggccgtcc ccttgtcgcg catctcgcac
      781 tgtttgtcgt cgagttcctt tatctccttc tggaaccgct ggccgcgctc gacgatggcg
      841 ttgagatcga cgacattgtt ccagttcggc ttgatgccgg tggtcttgca gacgaccgcc
      901 ttgatccgtt tgtcgcggaa caccgtgccg atgccgccgc ggcccgcctg tttgaggcgc
      961 gccaccttgc ggcgcgggtc ccagaaactg aaattgagca tgccgatgag cgaatggtcg
     1021 gcggcggtcc cggccgaaac gaccgcgaca ttgagcttgt ccttgtcgtc ctttgcgtac
     1081 atctcggtca actgttcggc gagcacgtga gaatcgatcg cctccttcgg cgccgtctcg
     1141 atggtgaccg aattgtcggt gccgtcgatg acgatgatga catcctcctg cgccttcccc
     1201 tgcagttcga gcgcgtcgaa gccggagaac ttcatatagg ggccgaaata gccgccgacg
     1261 ttgctgtcca tgatgccgcc ggtctgcggc gatatcgaca cgaccagcga tttcccggcg
     1321 cccgcgtact gggtgatgcc ggcgcagggg ccggggctga tgatgacctc gttctcgggg
     1381 tcgttccatt tggtctgtgg tctggtcgcg tgccagagca ggtagagccc gaatccttta
     1441 ccgccgacga acttgtcctt catcagcggg gtcaccggtt tttcggtgat ggtgttggtg
     1501 gtgaggtcga tgtggagggt gcggtcgttg tagccctttt cgatgcggcc gggtttgtat
     1561 tcccagcgcg tgacccgctt gtgtgccgcc ttgatctcgc cgatgttcat gccgtgctcc
     1621 atagatagaa cagaggttac taatacatat cacgccgccg agtatatcgg gaccttgctc
     1681 ctttttcaag gattttgtac gaagcgggag cggaagggct tactgacccg gtccgtgctg
     1741 gacgatcttc gcatcgaggc cgaggagctt cccgcacatg ccgctaccct ccgatgttgt
     1801 aaaaatcgcc ctgtgtgtta caggtaccct tcgccggttt cgccgcgatc gcgcgccgta
     1861 tcgcctcttc cgcgccgagt tcgcggacgc tgaagccgag atcggaaaag agacacggtt
     1921 tcaccgatcc gttcgcgagc aggcggatcc ggttgcaatg cgcgcagtcg ccgcccatgc
     1981 cgccgatgac accgtagaat tcgccgtggg caagatccat ccg
//
LOCUS       DAOQUE010000502         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_71176_length_2022_cov_4.360956, whole genome shotgun sequence.
ACCESSION   DAOQUE010000502 DAOQUE010000000
VERSION     DAOQUE010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71176_length_2022_cov_4.360956"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1008)
                     /locus_tag="PKH10_12340"
     CDS             complement(79..1008)
                     /locus_tag="PKH10_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNT28951.1"
                     /translation="MLSDIFMFEEHDEPIRSILAYYLSRGGERLTPRDSETRSFLTDI
                     PIIGSVGDAVADRTVHLVRTGAILVRGVADAAQAIVHRDIVRFSLFVLFFGEMLAKQR
                     AGTLPFAGKDLAHRLLAAVPELPAAPPPMLNGPFADEPAAQRAVVEAGNALVAAGLVD
                     SVFGNLSCRIGDTLLISRTGVPLDRLDGGVVACRLDGPVAPRGASSEYPSHRRIVTGN
                     RYRAVLHGHPRWTVIWSLASEDPRLFGLPVVEGAPGEELARVLPDAVAAHGAAIVRGH
                     GLFAAGIDDFRAPFETMDRVEHCAFADYLTEMS"
     gene            1157..>2022
                     /locus_tag="PKH10_12345"
     CDS             1157..>2022
                     /locus_tag="PKH10_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNT28952.1"
                     /translation="MSRLLGLFLVLGLATSCVTSGTHQLVVDELAAQKRANEELTTAN
                     EALKKEIADLQAQVAKLKKEFDEMSKNFMDAMTFNDDLKKKLKEKGASIEELDAQNKE
                     LAAKKEEEAKRAAEEQAKLESEKQALKAELEQLRRMKEAAEKRNAEFNNVMAKLKKMI
                     DAGTLSVKIRKGRMIVTLSSDILFAVGATELTPEGKAAIEELAATLKDIPDRNFLVVG
                     HSDSTPIKKKFASNWELSSQRAIEVVKLMIASGVPPVMISASGNAEFDPIAENDTPEN
                     KTLNRRVEIVLMP"
BASE COUNT          505 a          591 c          620 g          306 t
ORIGIN      
        1 accatgtaaa ggtcatgaag atgttcgaga tactcaacag aacgatggtt ctcatgtatg
       61 tctccgatga gggggtggtc aggacatttc ggtgagatag tcggcgaagg cgcaatgctc
      121 gacgcggtcc atcgtctcga acggtgcgcg gaagtcgtcg atgccggcgg cgaacagacc
      181 atgcccgcga acgatggcgg cgccgtgcgc ggcgaccgca tcaggcagga cacgggcgag
      241 ctcctccccc ggagcccctt ccacgaccgg cagaccgaac agcctcgggt cttcagaggc
      301 gagcgaccat atcaccgtcc agcgcgggtg gccgtgaagt acggcacggt aacggttccc
      361 ggtaacgatg cgacgatgcg acgggtattc gctcgacgcc ccgcgtggcg cgacgggacc
      421 gtcgagccgg caggcgacca cccctccgtc gagccggtca agcggcacac cggtgcgact
      481 gatgagcagg gtatcgccga tgcggcagga aaggttaccg aaaacggaat cgaccagccc
      541 cgccgccaca agagcgttcc ccgcctcgac gacggcgcgt tgcgccgccg gttcgtcggc
      601 gaagggtccg ttcagcatcg ggggcggggc ggccggtaac tccggcacag cggcaaggag
      661 ccgatgggcc agatccttcc cggcgaaggg aagcgttccg gcccgctgct ttgccagcat
      721 ctcgccgaag aaaagaacga acagggaaaa acggacgata tcacgatgaa cgatggcctg
      781 cgccgcgtcc gcaacgccac ggaccagtat cgcgccggtc cggacaagat gcacggtccg
      841 atcggcgacg gcatccccga cgctcccgat gatcgggatg tccgtaagaa aagacctcgt
      901 ctccgagtca cgcggcgtaa ggcgctcccc gccccgtgaa agatagtacg cgagtatcga
      961 acggatcggc tcgtcgtgct cttcgaacat gaagatgtcg gacaacacga gacacctcgc
     1021 agtcaatgcg tcgactatac gccgcgacac ggtaaagtca aaagcgaaaa aaaactccgt
     1081 ttttttgact tgcgggggcc ccgacggtac cataagaccg gtcatccgac catgaaaacg
     1141 gaaaaaggag ggcaccatgt cgagattgtt aggcttattc cttgtgttgg ggctggcgac
     1201 atcctgcgtc acgagcggca cccaccagtt ggtggtcgat gaactggcgg ctcagaaaag
     1261 ggccaacgag gagttgacca ccgcgaacga ggcgctcaaa aaggagatcg ccgatcttca
     1321 ggcgcaggtc gccaaactca agaaagaatt cgacgagatg tcgaagaact tcatggatgc
     1381 gatgaccttc aatgacgacc tcaaaaagaa actgaaggaa aaaggcgcct ccatcgagga
     1441 attggacgct cagaacaagg aactggccgc gaaaaaagag gaagaagcga aacgggccgc
     1501 cgaggagcag gcgaaactcg agtcggaaaa acaggcgctc aaggcggaac tggaacagtt
     1561 gcgccgcatg aaggaggcgg ccgagaaacg gaacgccgaa ttcaacaatg tcatggcgaa
     1621 actcaagaag atgatagacg ccggcacgct ctcggtcaag atccgcaagg gacgaatgat
     1681 cgtgacgctc tcatccgaca tccttttcgc ggtcggggcg accgaactca cccccgaagg
     1741 caaggcggcc atcgaggaac tcgcggcgac attgaaggac atccccgatc gtaatttcct
     1801 tgtcgtggga cactccgatt cgaccccgat aaagaagaaa ttcgcgtcga actgggaact
     1861 ctcctctcag cgcgccatcg aggtggtgaa actgatgatc gcaagcggcg ttccccccgt
     1921 catgatatcg gcctcgggca atgccgaatt cgacccgata gccgagaacg acacccccga
     1981 aaataagacg ctcaaccgcc gcgtcgagat agtcttaatg cc
//
LOCUS       DAOQUE010000503         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_71639_length_2014_cov_2.232261, whole genome shotgun sequence.
ACCESSION   DAOQUE010000503 DAOQUE010000000
VERSION     DAOQUE010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71639_length_2014_cov_2.232261"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKH10_12350"
     CDS             <1..408
                     /locus_tag="PKH10_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004276778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNT28953.1"
                     /translation="SYEVIMRVRPDALAGTGRSDYPNQVNNVLGFPAIFRGALDVRAS
                     AITDGMKVAASKALARIARLPVPEEVLGAYGLKELSFGPHYLIPKPFDLRVLVEEVCA
                     VAEAAVAEGVSRVADFDAATYRRSLEERFLTEG"
     gene            412..930
                     /gene="cobO"
                     /locus_tag="PKH10_12355"
     CDS             412..930
                     /gene="cobO"
                     /locus_tag="PKH10_12355"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNT28954.1"
                     /translation="MAERGMMQVYTGDGKGKTTAAIGLAVRALGAGKRVFIAQFVKGM
                     RYSELNLLERLEGLELRRYGRDCFIVKKPEQADIDLARQGLSEATAILKEGRYDLVIL
                     DEACIALYYHLFSLDELLAAIKGRAPRVEVVVTGRYAPQGLIDAADLVTEMREVKHYY
                     QKGVQAREGIEK"
     gene            complement(946..>2014)
                     /locus_tag="PKH10_12360"
     CDS             complement(946..>2014)
                     /locus_tag="PKH10_12360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT28955.1"
                     /translation="LDSFDEALPLARQCDPARVLSAEAPPGRSWGSLPEPYRDRLELL
                     LETIGRISWDSGIVPVRFDIPPDDPFHDLFAALTIMHNDILEIFARSEKSLLDAEEAT
                     RRKDRLIADVSHELRSPLTSIIVLSDMLALARDPQEACRYADQIRETALRMNRIIEDI
                     LSTALVGAGKGTMTSCRFDLAEQIAPVLDQFAMEASRKRIGFEKTVPEGALPVKGDPL
                     KVLQIVTNLLSNAVKYTDAGKVKVAIAVRDIDGRARKAFIEVSDTGIGISEEDQAHLF
                     SRFSRTPATASRRGIGLGLSIAHELACLMGGDIAVKSTPGKGSTFLFSFPFILDGSCT
                     VVTPKEEDGKSDTPSTRATTA"
BASE COUNT          365 a          596 c          668 g          385 t
ORIGIN      
        1 tcctacgagg tcatcatgcg tgttcgtccc gatgcgctcg ccggcaccgg ccggagcgat
       61 tatcccaatc aggtgaacaa tgtgctcggc ttccccgcca tattccgcgg cgcgctcgat
      121 gtccgtgcct cggcgataac cgacgggatg aaggtcgccg cctcgaaggc gctcgcccgg
      181 atcgcccgcc tgccggtccc cgaagaggtg ctgggcgcct acgggctcaa agaattgtcg
      241 ttcggcccgc actacctgat accgaaaccg ttcgatctac gggtgctcgt cgaggaggtg
      301 tgcgccgtcg ccgaagcggc ggtcgccgaa ggggtctccc gcgtggcgga cttcgacgcg
      361 gcaacttacc gccggtcgct tgaggaacgg ttcctgacgg agggatgacc gatggccgaa
      421 cgggggatga tgcaggtcta taccggcgac ggcaagggaa agacgacggc ggccatcggc
      481 ctggcggtcc gtgctctcgg cgccgggaag agggtcttca tcgcccagtt cgtcaagggg
      541 atgcgttaca gcgaactgaa cctgctcgaa aggctggagg gacttgagct acggcgctac
      601 ggtcgtgact gcttcattgt caagaagccg gaacaggccg atatcgacct cgcacgccaa
      661 ggtctttccg aggcgaccgc catcctgaaa gaggggagat acgacctcgt gattctcgac
      721 gaggcctgca tcgcgcttta ctaccacctc ttttcgctcg acgagttgct tgccgccatc
      781 aaaggacgtg cgccgcgggt cgaagtggtc gtcaccggtc gctatgcccc gcaggggctc
      841 atcgacgcgg ccgacctcgt caccgagatg cgcgaggtga agcactacta tcaaaagggc
      901 gttcaggccc gcgaggggat agagaagtag cctgtcggcg gcgtgtcagg cggtcgtcgc
      961 ccgggtcgag ggagtgtcgc tctttccgtc ctcttccttc ggcgtcacga ccgtgcatga
     1021 cccatccagt atgaagggaa aagagaagag gaaggtgctg cctttcccgg gtgtgctctt
     1081 tacagcgatg tcgcctccca tgagacaggc gagttcgtgc gctatcgaaa gcccgagccc
     1141 tatccctctc cgcgacgcgg tcgccggggt gcgtgagaag cgggagaaaa gatgggcctg
     1201 atcctcttcg cttatcccga tgccggtgtc ggatacctcg atgaacgctt ttcgggcccg
     1261 gccatcgata tctcgcaccg cgatagccac cttcaccttc ccggcgtcgg tatatttgac
     1321 ggcgttgcta agcaggttgg taactatctg gagcaccttg agcgggtcgc ctttcactgg
     1381 tagagcgcct tcggggacgg tcttttcgaa cccgatcctc ttgcgcgaag cctccatcgc
     1441 gaactggtcg agcacggggg cgatctgttc cgcgagatcg aaccggcaac tggtcatggt
     1501 ccctttaccc gctccgacca gagcggtgga gaggatgtcc tcgatgatac gattcatccg
     1561 cagggcggtc tcgcggatct ggtcggcgta acggcaggct tcctgcgggt cgcgggcgag
     1621 ggcgagcatg tcgctcagga cgatgatact ggtgagcggc gaccggagtt cgtggctgac
     1681 atccgctatc agacggtcct tccggcgggt cgcctcttcg gcatcgagaa ggcttttctc
     1741 cgaacgggcg aatatctcga gaatgtcgtt gtgcatgatc gtaagggccg cgaaaagatc
     1801 gtggaaaggg tcgtcggggg ggatgtcgaa ccttaccggc acaataccgc tgtcccacga
     1861 gatgcgcccg atcgtttcga gaaggagttc caagcggtcc cgatagggtt cgggtaacga
     1921 gccccaggac ctcccggggg gagcttcggc cgagagaacg cgggccgggt cacactgtcg
     1981 ggcgaggggg agcgcctcgt cgaaggagtc gaga
//
LOCUS       DAOQUE010000504         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_72110_length_2006_cov_3.249103, whole genome shotgun sequence.
ACCESSION   DAOQUE010000504 DAOQUE010000000
VERSION     DAOQUE010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72110_length_2006_cov_3.249103"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKH10_12365"
     CDS             <1..467
                     /locus_tag="PKH10_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28956.1"
                     /translation="YKKIIDHAYRVNPALKLSHKYFEVAGKFFWGQNLDVFGAFGGGI
                     VSETVDATERRVVDGQRTMGYWAQLTGKPIEGWTMHVGYGVEDPDERQEKASPLYFRN
                     SSIWVSTFYTFFEYVTLGFQWQQIRTEGLSVTGAPREDVTGNALMANAKLSF"
     gene            502..1323
                     /locus_tag="PKH10_12370"
     CDS             502..1323
                     /locus_tag="PKH10_12370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT28957.1"
                     /translation="MRQGVIALVTGAASGIGRAVVDELAVSGIKVYALDHDAVRLDES
                     VSHWRSSGHAVEGRVLDCSDREALAALAAEIDRVDGRLDILMLNAGILVGGPIGEIAE
                     ADIERIVAVNQMAVVRGVRVFLPLLRRTGPGAAIVITSSAAGLCAFPFVAPYCMTKFA
                     VAGFAESLAAELAPEGIAVLSVFPGMVRTAVFDNGRITLPGRGLEAVLSIFGRRALDP
                     ATMARRIVRAIRRRRRILTQLGAMWPLHLIKRLSPRGYQALWAFVTRRIVGRLEK"
     gene            1398..>2006
                     /locus_tag="PKH10_12375"
     CDS             1398..>2006
                     /locus_tag="PKH10_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT28958.1"
                     /translation="MAKKKHTMFVCRNCGSQSLKWLGRCPECGEWDSFDEKDETAFTV
                     SARDGKEPLIVHIADVATTPADRVDCASPEFNRILGGGAVRGSYNLISGAPGVGKSTL
                     MLQIALKLAHGRKVLYLSGEESAAQVKLRFGRLSRSDAQSGLFISNENNVDLLRGIVA
                     QEKFDVVFVDSIQSVFSSAVPGIPGSVSQIKDCAVRLLEMAKK"
BASE COUNT          347 a          614 c          662 g          383 t
ORIGIN      
        1 cctataaaaa gatcatcgat catgcttacc gcgtcaatcc ggcgctcaag ctgtcgcaca
       61 aatacttcga ggtggcgggc aagttcttct ggggccagaa tctcgatgtg ttcggcgcgt
      121 tcggtggcgg gatcgtgagc gagacggtcg atgccacgga gaggcgggtc gtcgatggtc
      181 agcgcaccat gggctactgg gcgcagttga ccggtaaacc gatcgagggg tggaccatgc
      241 atgtgggcta cggcgtcgag gacccggacg agcggcagga aaaagcgagc ccgctctatt
      301 tccggaactc ctcgatatgg gtgtcgacct tctacacctt cttcgaatat gtgacgctcg
      361 gctttcagtg gcaacagata cggaccgaag gcctgagcgt gaccggtgcg ccgcgcgagg
      421 atgtcacggg caacgccctg atggcgaacg ccaaactctc tttctgatcc ttcttcgatc
      481 aggacctttc gggagctctt catgcgtcaa ggggtcattg ctctggtcac cggcgccgca
      541 tcgggcatcg gtcgcgccgt tgtcgatgaa cttgccgttt cgggcatcaa ggtctacgcg
      601 cttgaccacg acgccgtacg gctcgacgag tcggtatcgc actggcgatc gtcgggacac
      661 gcggtcgaag gtcgcgtgct cgattgcagc gaccgagagg cccttgcggc gctcgccgcc
      721 gagatcgacc gcgtcgacgg gcggctcgac atactcatgc tcaacgccgg catcctcgtc
      781 ggcgggccga taggggagat agccgaggcc gacatcgagc ggatcgtcgc ggtgaaccag
      841 atggcggtcg tccggggcgt ccgcgtcttt ctgccgcttc tgcgccgcac cgggcccggc
      901 gccgccatcg tcatcacctc ttcggcagcc gggctgtgcg ccttcccgtt cgtcgctccc
      961 tactgcatga ccaaattcgc ggtcgccggg ttcgccgagt cgcttgccgc cgaactcgcc
     1021 cccgagggta tcgcggtgct ttccgtattc cccgggatgg ttcggacggc ggtattcgat
     1081 aacggtcgca tcacccttcc ggggcgcggc ctcgaggcgg tgctttcgat attcggtcgc
     1141 cgcgcgctcg accccgctac gatggcccgc cgcatcgtgc gggcgatacg gcggcggcgt
     1201 cgcatcctca cgcagttagg ggccatgtgg ccgctccatc tgataaagcg tctttctccc
     1261 cgcggctatc aggcgctctg ggcctttgtg acccgccgca tcgtcggacg tctcgaaaaa
     1321 taacgcggtc ccgctaaaaa ttgcctctgg tccccgcttg tgtcatactg cgccgaccgg
     1381 aacgggagga gttgttcatg gcgaagaaga aacacacgat gttcgtctgt aggaactgcg
     1441 gcagtcagtc gctcaagtgg ctcgggcgct gtcccgagtg cggcgagtgg gacagtttcg
     1501 acgagaagga cgagaccgcg ttcacggtga gcgcccgcga cgggaaggag ccgctgatcg
     1561 tacatatcgc cgatgtcgcg acgaccccgg ccgaccgggt ggactgcgca tcgcccgaat
     1621 tcaaccgcat cctcggcggc ggcgcggtgc gtggcagtta caacctcata tcgggcgcgc
     1681 ccggggtcgg caagtcgacg ctcatgctcc agatagcgct caaactggcg catggccgga
     1741 aggtgttgta cctgagcggc gaagagtcgg cggcgcaggt caaactgcgg ttcgggcggc
     1801 tcagccgcag cgacgcgcag agcgggctgt tcatcagcaa cgagaacaat gtggacctcc
     1861 tgcggggcat cgtggcgcag gaaaagttcg atgtggtgtt cgtcgattcg atacagagcg
     1921 tcttttcgag cgccgtgccc ggcatccccg gctcggtgag ccagataaag gattgtgcgg
     1981 tccgcctgct cgagatggcg aagaaa
//
LOCUS       DAOQUE010000505         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_72364_length_2001_cov_4.396197, whole genome shotgun sequence.
ACCESSION   DAOQUE010000505 DAOQUE010000000
VERSION     DAOQUE010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72364_length_2001_cov_4.396197"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PKH10_12380"
     CDS             complement(<1..483)
                     /locus_tag="PKH10_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HNT28959.1"
                     /translation="MGRIDLSLLLLLIALLPLSCEKGAGIPATGPMTGVKPLYDQTAD
                     HAYLSGQAGAQAGYAVASAGDVNGDGYDDLIVGAHYYDNGQTDEGAVFILHGSASGLS
                     TTPDTTIESDQAYTYFGISVAPAGDVNGDGFDDIVVGAYLYDHGQTDEGAAFVYLGSA
                     A"
     gene            complement(503..1294)
                     /locus_tag="PKH10_12385"
     CDS             complement(503..1294)
                     /locus_tag="PKH10_12385"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNT28960.1"
                     /translation="MRRPVEAAVREAGRILTSYFRNDTLTHEFKSPRDMVSEADRHAE
                     RYLMAELAKIDDIPFLSEEYNPRTALSTGPLWIIDPLDGTTNFLAGIPVYTIAVAHWD
                     GAKVDTAVCYIPPLDEMYRAEIGCGAFLNDAPIRVSDTADPLHAVGAVGFADITKGLP
                     NDTLGVFTSVIRKTRALRRLGSAAADMLYVARGGFDLFWECGLAPWDVAAASLIVTEA
                     GGIVTDFAGGDDFLAGGTIIASNRALYAFIRDEVHRNFGPLQDHR"
     gene            complement(1278..1583)
                     /locus_tag="PKH10_12390"
     CDS             complement(1278..1583)
                     /locus_tag="PKH10_12390"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNT28961.1"
                     /translation="MPIPRVAELEEVLTPTFLSLVSSMYSAVLYSLGELKLHGAPDTA
                     PNLDMARFNIGLLEILREKSAGNLSAEEARFLDDTITNAQAFLDKHTDGASPNAATS"
     gene            complement(1603..1791)
                     /locus_tag="PKH10_12395"
     CDS             complement(1603..1791)
                     /locus_tag="PKH10_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28962.1"
                     /translation="MSSAPKKTHPLAETDTRILERKLRKGEITREEFEKMLASLPDSG
                     DHVELDEQKISSVISKKD"
     gene            complement(1802..>2001)
                     /locus_tag="PKH10_12400"
     CDS             complement(1802..>2001)
                     /locus_tag="PKH10_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28963.1"
                     /translation="GSLGEDDDGGEDDGGVATKWWFWTIIGTVVAGGVTTGCLLAIPG
                     SPAYLGKKSDGGATMTIHFGQ"
BASE COUNT          318 a          598 c          703 g          382 t
ORIGIN      
        1 ggcggcggac cccagataga caaaggccgc tccctcgtcg gtctggccat ggtcgtagag
       61 ataggcgccg acgacgatat cgtcgaaccc gtcgccgttc acatcgcccg ccggggcgac
      121 cgaaatgccg aagtatgtgt aggcctgatc ggactcgatg gtggtgtcgg gggtcgtgct
      181 gaggccggag gccgagccgt gtaagatgaa gaccgcccct tcgtcggtct ggccgttgtc
      241 gtagtaatgg gcgccgacga tgagatcgtc gtagccgtcg ccgttcacat cgcccgccga
      301 tgcgaccgca tagccggcct gggcgcctgc ctgtcccgac aggtatgcgt ggtcggccgt
      361 ctggtcgtag agcggcttaa cgccggtcat cggcccggtc gcggggatcc ccgccccctt
      421 ttcacaggaa agagggagga gggcgatcaa gagaaggagc agggaaaggt cgatgcgtcc
      481 catggtcgta ctccgttatc ggttatcgat ggtcctgtag cggcccgaag ttgcggtgca
      541 cctcgtcgcg gatgaatgcg tacagcgcgc ggttcgaggc gatgatcgtg ccgcccgcga
      601 ggaagtcgtc gccccccgcg aagtcggtca cgatgccgcc cgcctcggtg acgatgaggc
      661 tcgccgccgc gacatcccac ggcgcgaggc cgcattccca gaagaggtcg aacccgccgc
      721 gagccacata gagcatgtcg gccgccgccg agccgagccg ccgcagggcg cgggtcttgc
      781 ggatgacgct ggtgaagacg ccgagcgtat cgttcggcag tcccttggtg atgtcggcga
      841 atccgaccgc cccgacggca tggagcgggt cggcggtgtc gctcacccgt atcggcgcgt
      901 cgttgaggaa cgccccgcac ccgatctcgg cacggtacat ctcgtccagc ggcgggatgt
      961 agcagacggc ggtgtcgacc ttcgcgccgt cccagtgggc gaccgcgatg gtgtaaacgg
     1021 ggatgccggc gaggaaattg gtggtgccgt cgagcgggtc gatgatccag agcggcccgg
     1081 tagaaagcgc tgtgcgcggg ttatattcct cgctcaggaa cgggatatcg tctatcttcg
     1141 cgagttccgc catcaggtat cgttcggcgt gacgatcggc ctcgctgacc atgtcgcgcg
     1201 gcgatttgaa ctcgtgggtg agcgtgtcgt tccggaaata ggaggtaagg atgcggccag
     1261 cctcgcggac ggccgcctca actggtcgcc gcattgggcg aggcgccgtc ggtgtgcttg
     1321 tcgaggaatg cctgcgcgtt ggtgatggtg tcgtcaagga accgcgcctc ctccgcgctc
     1381 aggttcccgg cgcttttctc gcgcagtatc tcgagcagtc cgatattgaa ccgggccatg
     1441 tcgaggttcg gcgccgtgtc gggagcgccg tgcagtttga gttcgccgag actgtagagc
     1501 accgccgagt acatcgagga gacgagactg aggaaggtgg gggtgaggac ctcttcaagt
     1561 tcggcgacgc gcggtatcgg catggggcct cccgggtatc ggtcagtcct ttttcgagat
     1621 gaccgaggag atcttctgtt cgtccaactc gacatggtcg ccgctgtcgg gcaacgaggc
     1681 gagcatcttc tcgaactctt cgcgggttat ctcgcccttg cggagtttcc gttcgagtat
     1741 gcgggtatcg gtctccgcga gcggatgggt ctttttgggt gctgatgaca tcggggactc
     1801 cttattggcc gaagtgtatc gtcatggtcg cgccgccgtc gctcttcttg ccgaggtagg
     1861 cgggagagcc gggtatggcg agcaggcatc cggtcgtgac gccgccggcg accacggtgc
     1921 cgatgatggt ccagaaccac catttggtgg ccacgccgcc gtcgtcctcg ccgccgtcgt
     1981 cgtcctcgcc gagcgaaccg g
//
LOCUS       DAOQUE010000506         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_72412_length_2000_cov_4.604113, whole genome shotgun sequence.
ACCESSION   DAOQUE010000506 DAOQUE010000000
VERSION     DAOQUE010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72412_length_2000_cov_4.604113"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2000
                     /locus_tag="PKH10_12405"
     CDS             <1..>2000
                     /locus_tag="PKH10_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNT28964.1"
                     /translation="KVGEFLIKAKIREGQVVLIGGVTQNRHLIGFLREAWPKVEFVTP
                     LEACWFEAYGAAIAARERGRALPAADLLRPRAQSEHGLYRPLATARDQVTYHQAHRGS
                     VKPGHEYILGIDGGSTTTKAALIDRETLELTAAHYGRTHGDPVGALKECLRRIREQCG
                     GVRPSITLAATTGSSRELLGVFVGTDGVYNEIIAHATGTAHFAAEVDTLFEIGGQDAK
                     YVLLNNGVPIDYAMNEACSAGTGSFLEESAAGDLDIGRAEDIGPIALEARAPLKFGEH
                     CSAFINSDIRKAIQEGSERADIVAGLVFSIVSNYLNRVVGNRTIGDRIALQGGVAKNP
                     AVPLAFAQLLGKHITVPPDPELMGSFGVARLARRKNEDGLLPEGTFDLDTLIEKDIVN
                     HGEFVCRACDNYCTIRNLEAGGKRYPFGGRCSKYTGARRRTSFDESSIADHVAWRTEQ
                     HFSTQVPDPASLTRRSPAVVGVPVAFSVHSLWPFYAQFFHELGVRTVLSEKVLPEGVY
                     RQESNYCFPAEIAHGAIQDLIDRGADHLFLPHFRDMPTMEEKTAQSCVCPLTQGLPYY
                     AKQAFGLDDAKILRPVVSFKEGWDACRPEFEDVAEKLGLPRSVGRNAFDTAIAHYRDF
                     MAAYKKRGRELVEEMRANPDRLYVALLGRPYNAFTRDANMGI"
BASE COUNT          374 a          682 c          615 g          329 t
ORIGIN      
        1 caaggtgggc gagttcctca tcaaggcgaa gatacgcgaa ggacaggtcg tgcttatcgg
       61 cggcgtcacg cagaaccgtc atctcatcgg tttcctgcgc gaagcgtggc cgaaggtgga
      121 gttcgtcacg cctcttgagg cctgctggtt cgaggcgtac ggcgcggcga tagcggcccg
      181 cgaacgggga cgggcgctcc cggccgctga cctcctgcga ccgcgcgcac agagcgaaca
      241 cgggctgtac cggccgcttg cgaccgcccg ggaccaagtg acctaccatc aggcacaccg
      301 cggctcggtg aagcccggcc acgaatatat actcggcatc gacggcggct cgacgacgac
      361 caaggcggcg ctcatcgacc gcgagacgct cgaactgacg gcagcccact acggccgtac
      421 ccacggcgat ccggtcggcg cgctcaagga atgtctgcgc cggatacggg agcaatgcgg
      481 cggcgtccgt cccagcatca cgctcgcggc gaccaccggc tcgtcgcgcg aactgctcgg
      541 ggtcttcgtc ggcaccgacg gcgtctataa cgagatcatc gcccacgcga ccggcaccgc
      601 ccatttcgcc gccgaggtcg ataccctctt cgagatcggc ggacaggacg cgaaatatgt
      661 actgctcaac aacggcgtcc cgatagacta cgcgatgaac gaggcctgct cggccggcac
      721 cggttcgttc ctcgaggaat cggccgcggg cgatctcgac atcggccgcg ccgaggatat
      781 cggtcctatc gcgcttgagg cacgggcgcc gctcaaattc ggcgaacact gctccgcctt
      841 catcaatagc gatatccgca aggcgataca ggaaggatct gaacgggccg acatcgtcgc
      901 cgggctcgta ttctccatcg tttcgaacta tctgaaccgt gtggtgggca atcgcaccat
      961 cggcgaccgc atcgccctgc agggaggcgt cgcgaagaac ccggcggtgc cgctcgcctt
     1021 cgcacaactg ctgggcaaac acatcaccgt gccgcccgac ccggaactca tgggttcctt
     1081 cggcgtcgcg cggctcgcac ggcggaagaa cgaggacggc ctgctcccgg aggggacgtt
     1141 cgatctcgat acgctcatcg agaaggatat cgtgaaccat ggcgagttcg tatgccgcgc
     1201 ctgcgacaat tactgcacga tacgcaacct cgaggctggg gggaagcggt accccttcgg
     1261 cggcagatgc tccaaataca ccggcgcacg gcgacgcacc tcgttcgacg agtcgagcat
     1321 cgccgaccat gtcgcatggc gcaccgaaca gcacttctcg acgcaggtcc ccgaccccgc
     1381 ctcactcacc cggcgctccc ccgccgtcgt cggtgtcccg gtcgccttct ctgtccattc
     1441 gctctggccg ttctatgcgc aattcttcca cgaactcggc gtgcgcaccg tgctttccga
     1501 aaaggtactc cccgaggggg tctaccggca ggagagcaat tactgctttc cggccgagat
     1561 cgcccatggc gcgatacagg atcttataga tcgcggcgcc gaccacctgt tcctgccgca
     1621 cttccgggac atgccaacga tggaggaaaa gacggcacaa tcctgcgtct gcccgctcac
     1681 gcaggggctt ccctactacg cgaagcaggc gttcgggctc gacgatgcga agatcctgcg
     1741 tccggtcgtc tcgttcaagg agggctggga tgcgtgccgt cccgaattcg aagatgtcgc
     1801 cgagaaactc gggcttcccc gctcggtggg acgcaacgcg ttcgacacgg cgatcgctca
     1861 ctaccgtgac ttcatggcgg cctacaagaa gcgggggcgc gaactcgtcg aggagatgcg
     1921 cgcgaacccc gaccggctct atgtggcgct cctcggcagg ccctacaacg ccttcacccg
     1981 cgacgccaac atgggcatcc
//
LOCUS       DAOQUE010000507         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_72676_length_1996_cov_2.518290, whole genome shotgun sequence.
ACCESSION   DAOQUE010000507 DAOQUE010000000
VERSION     DAOQUE010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72676_length_1996_cov_2.518290"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PKH10_12410"
     CDS             complement(<1..631)
                     /locus_tag="PKH10_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNT28965.1"
                     /translation="MTRPLVMKFGGSSLADTEKIGAIADIVVRRRRQDAPVVVVVSAM
                     GRTTDGLIEQARDFSQRPDLREMDMLLTAGERISAALVAIALNARGVRAVSLTGSQAG
                     IITDDAHTNARIIEVRPFRVKKALEEGCVVVVGGFQGVSFSKEVTTLGRGGSDVSAVA
                     LAAALGAATCEIYSDVTGVYAADPRIVKEPERIAALGYQEMQELAEAGVD"
     gene            complement(628..1140)
                     /locus_tag="PKH10_12415"
     CDS             complement(628..1140)
                     /locus_tag="PKH10_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28966.1"
                     /translation="MKKILFIGLTAAILGACGGPASYSTPLVMQNRIVAQSVCEGSDA
                     IDPFDDADALFGADDALPTPSVSVEVQNDTVVMRHENLYLPRGTLLGIEDRDGRLTWR
                     DARPYYYLTADTDFIAVRESARLVAADEFCLYSLTVTIQNISGGEYTFYLFDHTGAAV
                     PEATSQLKVR"
     gene            complement(1137..1706)
                     /locus_tag="PKH10_12420"
     CDS             complement(1137..1706)
                     /locus_tag="PKH10_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNT28967.1"
                     /translation="MKSLLLHACCAPCLSAVSDVLYNGFDGKFAVLFYNPNIDTRAEH
                     DRRLREVERYLVARYGKNVELLSGGYEQDRFEREVVPFAATGERGERCTLCYAMRLSR
                     TFEEAAARGMTHAATTLTVSPHKDAARINKMGLALERHHAVRYLSGTWEYRRSFDLCR
                     EYAIYRQDYCGCRESATERDQRKKKRGAS"
     gene            complement(1703..>1996)
                     /locus_tag="PKH10_12425"
     CDS             complement(1703..>1996)
                     /locus_tag="PKH10_12425"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT28968.1"
                     /translation="TEIQATLKKKPVIYEVVYHLYLPAPEGPYRIFDIELDGVSMLRN
                     YREQFSKTLKESGIDELIKKIDARLARKKDKDAAKGSSPITPKKKEEAKTPPQ"
BASE COUNT          347 a          626 c          626 g          397 t
ORIGIN      
        1 catcgacgcc cgcctcggcg agttcctgca tctcctggta gccgagcgcc gctatccgtt
       61 ccggttcttt gacgatacgg ggatcggcgg catagacccc ggtcacatcg gagtatatct
      121 cgcaggtggc ggcgccgagc gccgccgcga gggcgaccgc cgaaacatct gaacctccgc
      181 gccccagcgt cgtgacctct ttggaaaaag atacgccctg aaacccgccg acgacgacga
      241 cgcacccctc ttcaagcgcc tttttcacgc ggaagggacg aacctctatg atacgggcgt
      301 tggtatgggc gtcatcggtt atgatgccgg cctgcgaacc ggtcagcgag accgcccgga
      361 cgccacgcgc attgagcgct atcgccacga gcgccgcgga aatgcgttcg ccggcggtga
      421 gcagcatgtc catctcgcgc aggtcgggtc tctgcgagaa atcgcgggcc tgttcgatga
      481 gtccgtcggt cgttctcccc atcgccgaga ccaccaccac gaccggagca tcttggcggc
      541 ggcggcgcac gacgatatcg gcgatcgctc ctatcttttc cgtatcggcg agcgaggagc
      601 cgccgaactt catgacgagg gggcgggtca tcgtaccttc agttgggagg tcgcctccgg
      661 caccgcggcg ccggtatggt cgaacagata gaaggtgtat tcgccgcccg atatgttctg
      721 tatcgtcacg gtgagcgagt agaggcagaa ctcatcggcg gcgaccagtc gcgccgattc
      781 gcgcaccgct atgaagtcgg tatcggccgt caggtagtag tacgggcggg catcgcgcca
      841 ggtcaaccgg ccatcgcggt cctcgatgcc gagaagcgtc ccgcgcggga ggtagagatt
      901 ctcgtgccgc atgaccaccg tatcgttctg gacctcgacc gacaccgagg gcgtcggcag
      961 ggcgtcatcc gccccgaaca gggcatccgc gtcgtcgaac gggtcgatgg cgtcgctccc
     1021 ctcgcagacg ctctgcgcga cgatgcggtt ctgcatcacg agcggcgtcg aataggaggc
     1081 aggaccgccg caggcgccga ggatcgccgc cgtcagaccg atgaacagta tctttttcat
     1141 gacgcacctc tttttttctt ccgttggtcg cgctcggtgg cgctttcacg gcatccgcag
     1201 taatcctgtc ggtagatggc gtattcccgg cacagatcga aagatcggcg atattcccat
     1261 gtcccggaaa ggtagcggac ggcatgatgc cgctcgagcg caagccccat cttgttgatg
     1321 cgcgccgcgt ccttgtgcgg actgacggtg agggtggtgg ccgcatgggt catgccgcgc
     1381 gcggcggcct cctcgaaggt ccgcgagagc cgcatggcgt aacagagcgt acagcgctcc
     1441 ccccgttcgc cggtcgcggc gaaggggacc acctcccgct cgaaacggtc ctgctcatat
     1501 ccgccgctca aaagttcgac attcttcccg tagcgggcga cgagatagcg ctccacctca
     1561 cgcaggcgcc ggtcgtgttc ggcacgggta tctatgttcg ggttgtagaa cagcacggcg
     1621 aatttcccgt cgaacccgtt atacagcaca tcggaaacgg ccgacagaca gggagcgcaa
     1681 caggcgtgaa ggagcagact cttcattgcg gcggcgtttt cgcctcctct ttcttcttcg
     1741 gcgtgatggg cgaactcccc tttgcggcgt ccttatcctt cttgcgggcg agccgggcgt
     1801 ctatcttctt tatcagttcg tcgataccgc tctctttgag cgtcttggag aactgttcgc
     1861 ggtagttgcg tagcatgctc acgccgtcga gttcgatgtc gaatatgcgg tagggacctt
     1921 ccggcgcagg gaggtacagg tgatagacca cctcgtagat gaccggcttt ttcttcaggg
     1981 tcgcctgtat ctcggt
//
LOCUS       DAOQUE010000508         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_73157_length_1987_cov_4.169772, whole genome shotgun sequence.
ACCESSION   DAOQUE010000508 DAOQUE010000000
VERSION     DAOQUE010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73157_length_1987_cov_4.169772"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKH10_12430"
     CDS             complement(<1..246)
                     /locus_tag="PKH10_12430"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNT28969.1"
                     /translation="MIAVLQRVSRASVTVEQHIIAEIGPGLLLLAGLEKSDGDDDLDH
                     IARKCVELRIFPDSEGKMNRSVLESGGSILAVSQFTLA"
     gene            complement(250..1188)
                     /gene="arcC"
                     /locus_tag="PKH10_12435"
     CDS             complement(250..1188)
                     /gene="arcC"
                     /locus_tag="PKH10_12435"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNT28970.1"
                     /translation="MKIVIALGGNALIKPGEKGTPEEQLRNIENSVDGIVKLITEGHQ
                     VVVTHGNGPQAGARLIRNEMAATEVFPDPLVVCVADTQGSMGYMIEQGLVNRLIKAGV
                     RPRIATLVTQIEVNPSDPAFSKPTKPVGPFYTQEKAIAKMKSGWKMVEDAGRGYRRVV
                     PSPMPLRMVNPEALASLLEHGFTVITGGGGGIPVYRDKTGHLAGIDAVIDKDLASVMI
                     ARSIQADIVVILTGVEKVAINFKSGNPTWIDRMSVREAKSYLAQGEFPAGSMGPKIQA
                     AIDFAEAFRDRKVIITSESKLTEALAGTTGTVIENG"
     gene            complement(1263..>1987)
                     /locus_tag="PKH10_12440"
     CDS             complement(1263..>1987)
                     /locus_tag="PKH10_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNT28971.1"
                     /translation="GVDYEAILRTAEKTDNPDVILWDGGNNDMPFFDSDLKIVVVDPL
                     RPGHEENYYPGEANLRMADVVVINKMDSASPEGLKTVRDNIKRLAPNAKVVEADSELT
                     VSGDIKDKLVLVVEDGPTLTHGEMKIGAASVAAKRFGAKKMADPRPHAVGKIKYTYEV
                     YPNIGEGILPAMGYSAEQKKDLETTINNTPADLVLSGTPIDLSRLVKANKPVVRVDYA
                     LKCRAGFEKVIETAIKEKLFKK"
BASE COUNT          319 a          597 c          620 g          451 t
ORIGIN      
        1 ggcgagcgtg aattggctca ccgcgagtat cgagccgccg ctttccagca ccgaacggtt
       61 catcttgcct tcactgtcgg ggaagatgcg cagttcgacg catttgcggg cgatgtggtc
      121 cagatcgtcg tcaccgtcgc ttttttcaag tcccgcaagc aggagcagcc ccgggcctat
      181 ctcggcgatg atgtgttgtt cgaccgtgac cgaagcgcgg gaaacgcgct gaaggacggc
      241 gatcatgggc tatccgttct cgatgacggt gccggtcgtc ccggcgagcg cctcggtcag
      301 tttcgattcg gaggtgatga tgaccttgcg gtcgcggaac gcttcggcga aatctatcgc
      361 cgcctgtatc ttcggtccca tgctacccgc cgggaactcg ccctgcgcga gataactttt
      421 cgcctcgcgc accgacatcc ggtctatcca ggtggggttg cccgacttga agttgatggc
      481 gaccttttcg acgccggtga gtatgacgac gatatcggcc tgtatcgatc gtgcgatcat
      541 gaccgaggcg agatccttgt cgatgaccgc gtcgatgccc gcaaggtgcc ccgtcttgtc
      601 gcgatagacc ggtatgccgc cgccgccgcc ggtgatgacg gtgaagccgt gttcgagaag
      661 cgaggcgagc gcctcggggt tcaccatgcg aagcggcatt ggcgagggaa cgacgcgtcg
      721 gtagccccgc ccggcatcct cgaccatctt ccagccgctc ttcatcttgg ctatcgcctt
      781 ttcctgcgtg tagaagggac cgaccggctt ggtcggcttg gagaaggccg gatcgcttgg
      841 attcacctct atctgagtga cgagcgtcgc gatacgcggc cggacacccg ccttgatgag
      901 ccggttgacg agtccctgct cgatcatgta ccccatactt ccctgcgtat cggcgacgca
      961 gacgacgagc gggtcgggga acacctcggt cgccgccatc tcgttgcgta tcagccgcgc
     1021 gccggcctgc ggaccgttgc catgcgtgac gacgacctga tggccttccg ttatgagttt
     1081 cacgatgccg tcgaccgaat tctcgatgtt gcgcaactgc tcctcgggcg tccctttctc
     1141 gccgggcttg atgagcgcgt tgccgccgag cgcgatgact atcttcacga aggacctcct
     1201 tacagcagaa aaagaaaaga gggccgtgac accctctttt cccgtggatc ttgcggtgcg
     1261 atctacttct tgaacagttt ttcctttatc gcggtctcga tgaccttctc gaaccctgcg
     1321 cggcacttga gcgcgtagtc gacacggacg acgggcttgt tcgccttcac gaggcgggac
     1381 agatcgatcg gggtgccgga aagtacaagg tcggccggcg tgttgttgat cgtcgtttcg
     1441 aggtccttct tctgttcggc cgagtagccc atcgcgggga gtatgccttc gccgatgttc
     1501 gggtacacct cgtaggtgta ctttatctta ccgacggcgt gcgggcgcgg gtcggccatc
     1561 tttttcgcgc cgaacctttt tgcggcgacc gacgcggcgc cgatcttcat ctcgccgtgg
     1621 gtgagcgtcg gaccgtcctc gacgacgagc acgagtttgt ccttgatgtc gcccgatacg
     1681 gtgagttcgc tgtcggcctc gaccacttta gcgttcgggg cgagccgctt gatgttgtcg
     1741 cgcacggtct tgagtccctc gggggacgcc gagtccatct tgttgatgac cacgacatcg
     1801 gccatgcgaa ggttcgcctc tcccgggtag taattctctt cgtgtcccgg acgcagggga
     1861 tcgacgacga ctatcttgag gtcgctgtcg aagaacggca tgtcgttgtt gccgccgtcc
     1921 cagaggatga catcggggtt gtcggtcttc tcggcggtgc gaaggatcgc ttcgtaatcg
     1981 acaccgg
//
LOCUS       DAOQUE010000509         1960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_74761_length_1960_cov_3.685564, whole genome shotgun sequence.
ACCESSION   DAOQUE010000509 DAOQUE010000000
VERSION     DAOQUE010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74761_length_1960_cov_3.685564"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PKH10_12445"
     CDS             complement(<1..565)
                     /locus_tag="PKH10_12445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNT28972.1"
                     /translation="MIYLVKRELRLTDDHLIAVSEEEAHYLFKVRRVGREEPVFFYHE
                     GIVYETALAEMSRKEGLFEIRARHDVARRGRRVFVAIAAIEWSRLEEAVRNGVEAGAH
                     GFFLFRAGRSNVPETAMLKRVQRIEQIIVSAASQSQRRCLPDIRGAGREVIVKRPGRH
                     IVFHPEADRPVTPEHFAGDGDLYLWIGP"
     gene            complement(567..1328)
                     /locus_tag="PKH10_12450"
     CDS             complement(567..1328)
                     /locus_tag="PKH10_12450"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT28973.1"
                     /translation="MIKLSIETTKRSIREQYFPIGPASDIFVNLALLPNYETIKVGGA
                     VEIRFVVTDEKSEVLIRGQVSWKRPREIQLPGRVMPAGIGVKLDEKSYDLIEERILKE
                     ESELSDLSMMMVGGNYIRVRHDIAQVLKARDANRPTLPPDKKGGDQRRQPRVGISVPI
                     EVFINEQVANFKTRDISMCGMSFETAEPLKEGEEVVIILEDRTVHKQFILKARVARHI
                     PHPDDARRIIGVGVLFLFESDKQQKELMNFIIRNS"
     gene            complement(1325..>1960)
                     /gene="ftsY"
                     /locus_tag="PKH10_12455"
     CDS             complement(1325..>1960)
                     /gene="ftsY"
                     /locus_tag="PKH10_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNT28974.1"
                     /translation="GPLCGHHKRPMVLLMIGVNGAGKTTTIGKLARRFIADGHRVTLA
                     AGDTFRAAAVEQLSAWGVRTGAEVISEKEGADPAAVAFNAVNSAVAGGRDIVIIDTAG
                     RLQNRVNLMEELRKVHRVLGKALDGAPHETILVIDANNGQNAVSQAREFGEAVPITGI
                     IATKLDGTAKGGALVGIARELAIPVYFAGIGEAADDLRPFSAEAFTEALFS"
BASE COUNT          324 a          655 c          596 g          385 t
ORIGIN      
        1 cgggcccgat ccacagatag agatcgccgt cgcccgcgaa gtgttcgggg gtcaccggtc
       61 gatcggcctc ggggtggaac acgatatggc gacccggtcg cttgacgatg acctcacgcc
      121 ctgcgccgcg gatgtcgggc aggcaacgac gctgactctg cgaagcggcc gaaacgatga
      181 tctgctcgat gcgctggacc cgtttgagca tcgccgtttc gggcacatta ctgcggcccg
      241 cgcggaacag gaagaacccg tgcgcccccg cctcgacccc gttgcggacc gcctcctcga
      301 gccgcgacca ttcgatggcg gcaatcgcca cgaacacccg gcgcccccgc cgtgcgacat
      361 cgtgacgggc gcgtatctcg aacagaccct ccttgcgcga catctcggca agcgcggtct
      421 cgtacacgat cccttcgtgg tagaagaaca ccggctcttc ccgcccgacc cggcgcacct
      481 tgaagagata gtgggcttcc tcctcgctca ccgcgatcag gtggtcgtcg gtaagtcgca
      541 gttctctctt gacgagatag atcactctag gaattccgga tgatgaagtt catcagttcc
      601 ttctgctgtt tgtcgctttc gaacaggaac aacaccccca ccccgatgat acgccgcgca
      661 tcgtcgggat gcgggatatg gcgcgccacc cgcgccttga ggatgaactg tttgtgaacg
      721 gtgcggtcct cgaggatgat gacgacctcc tcgccctcct tgagcggttc ggcggtctcg
      781 aacgacatgc cgcacatgct gatgtcgcgc gtcttgaagt tcgcgacctg ttcgttgatg
      841 aacacctcta tcggcaccga gatgcccacg cggggctggc gccgctggtc accccctttc
      901 ttgtcgggcg gcagggtggg gcggttcgcg tcgcgggctt tgaggacctg cgcgatatcg
      961 tgccgcaccc ggatgtagtt gccgccgacc atcatcatac tgaggtcgga cagttcgctc
     1021 tcttctttga gtatacgttc ctcgatgaga tcgtaactct tctcgtcgag tttcactccg
     1081 atgccggcgg gcatcacgcg ccccggcagt tgtatctcgc gcggccgttt ccacgagacc
     1141 tgcccgcgga tgagcacctc gctcttctcg tcggtcacga cgaaccggat ctcgacggcg
     1201 ccccccacct tgatggtctc gtaattcggg agcagggcga gattcacgaa gatgtcggac
     1261 gccggaccga tcgggaaata ctgctcgcgg atcgaccgtt tggtcgtctc gatactcagt
     1321 ttgatcatga gaacaacgcc tccgtaaaag cctcggcact gaaaggtcgc aggtcgtcgg
     1381 ccgcctcgcc gatgccggcg aaatagaccg gtatagcgag ctcccgcgcg atgccgacga
     1441 gcgcgccgcc ttttgccgtg ccgtcgagtt tggtcgctat gatgccggtg atcggtacgg
     1501 cctcgccgaa ctcgcgggcc tgcgacaccg cgttctgccc gttgttggcg tcgatgacca
     1561 gtatcgtctc gtgcggcgca ccgtcaagcg ccttgccaag gacacggtgc accttacgca
     1621 gttcctccat cagattgacg cgattctgca ggcgccccgc cgtatcgatg atgacgatat
     1681 cgcgaccgcc ggcgaccgcc gagttcacgg cgttgaaggc gaccgccgcc ggatcggcgc
     1741 cttccttctc gctgatcacc tcggcgccgg tccgtacgcc ccacgccgag agttgttcga
     1801 ccgcagcggc ccggaaggtg tcgcccgccg cgagcgtcac gcggtgaccg tcggcgatga
     1861 agcgccgcgc cagtttcccg atggtggtcg tcttgcccgc gccgttcaca ccgatcatga
     1921 gtaggaccat gggccgcttg tggtgcccgc acagcggccc
//
LOCUS       DAOQUE010000510         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_75134_length_1954_cov_3.721432, whole genome shotgun sequence.
ACCESSION   DAOQUE010000510 DAOQUE010000000
VERSION     DAOQUE010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75134_length_1954_cov_3.721432"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PKH10_12460"
     CDS             complement(<1..583)
                     /locus_tag="PKH10_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28975.1"
                     /translation="MNRFFWMLATLSCLLIACGDDGGTQDPVDTEPVDDAVVPDDAAQ
                     PDTEQPDEVLLDTEPDGDGLLVPDDDAPDTVPVYCTLAANATFAYIYDGNGDLQAQLN
                     ANQSGYLTSAVFTGSLGDAEKPVPPAAAVQTIAFSYHLAASGGQPAQIIVTQQSFDGA
                     HAVVNPFVQMSPPNDAITTGQFSVDASKAGSGYV"
     gene            698..>1954
                     /locus_tag="PKH10_12465"
     CDS             698..>1954
                     /locus_tag="PKH10_12465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNT28976.1"
                     /translation="MLSILKSGAVSGISGFLIDVEVDIARGMPAFTIVGLPDTAIKES
                     RERVRTAVINSGHNFPQNRLTINLSPASVPKEGAGFDLPIAVALMAHVHDLPVEPVSR
                     YLLIGELGLNGEVKPVPGILPLAIAARDHGLTGLIVPEKNAREAAAISAVKVIAVSHI
                     NQILEILRGAEPYLFVPDRRAEVVRRHPDFSDVKGHEQAKRAIEVAAAGGHNLLMIGS
                     PGSGKTMLARRIPSVLPLLEEEERIETTKIFSVAGLLAEEGLIVERPFRSPHHTLSDV
                     ALAGGGVPPRPGEISLAHNGVLFLDEFPEFKKNALEVLRQPLEDGEITISRATSTVKF
                     PSSFMLVASMNPCPCGYLFDRKHPCTCSQVAIQKYQHKISGPLLDRIDISVIVNSVPY
                     EDLRKKGGGEPSDKLRDRIERARTVQA"
BASE COUNT          352 a          601 c          648 g          353 t
ORIGIN      
        1 tcacatagcc ggagcccgcc ttgctggcgt ccaccgagaa ctggccggtg gttatcgcgt
       61 cgttcggggg gctcatctgg acgaacgggt tgaccacggc atgggcgccg tcgaaactct
      121 gctgggtgac gatgatctgc gcgggctgac cgcccgacgc cgcgagatgg tacgaaaagg
      181 cgatggtctg tacggccgcc gcgggcggga ccggtttttc ggcatcgccg agactgccgg
      241 tgaagacggc ggaggtgaga tagccgctct ggttcgcgtt gagttgggcc tgcaggtcgc
      301 cgttgccgtc gtagatgtag gcgaaagtgg cgttcgccgc gagcgtgcag tagaccggta
      361 cggtgtcggg cgcgtcgtca tcggggacga ggagaccgtc gccgtccggt tcggtgtcga
      421 gcagtacctc gtcgggctgt tcggtatcgg gttgcgccgc gtcgtcggga acgacggcgt
      481 catcgaccgg ctcggtatcg accgggtcct gcgtaccgcc gtcatcgccg cacgcgatca
      541 gaagacacga aagagttgca agcatccaaa agaatctgtt catgtttctc ctcccacctt
      601 aagttatgga gtgattatag gcgggaataa gatatttgca aatcaaattt tcttttaata
      661 tagtcgccgc ggtttatgtt ccgggaggga tgttctcatg ctgtccattc tcaaaagcgg
      721 cgccgtttcg ggcatttcgg gctttctcat cgatgtcgag gtcgatatcg cccgcggcat
      781 gcccgccttc accatcgtcg gactccccga cacggcgatc aaggagtcgc gcgagcgggt
      841 ccgcaccgcg gtcatcaaca gcggccacaa cttcccgcag aaccggctga cgataaacct
      901 ctcccccgcg agcgtcccga aagagggggc ggggttcgac ctgccgatcg cggtagcgct
      961 catggcccat gtccacgacc tgccggtgga gccggtgagc cgctatctgc tcatcggcga
     1021 actggggctg aacggcgagg tgaagccggt gccgggcatc ctgccgctcg ccatcgcggc
     1081 gcgcgaccac gggctcacgg ggctcatcgt tcccgaaaag aacgcccgcg aggcggccgc
     1141 catcagcgcg gtcaaggtga tcgccgtgtc gcacatcaat cagatactcg agatcctgcg
     1201 cggcgccgaa ccgtacctgt tcgtccccga ccggcgcgcc gaggtggtgc gccgccaccc
     1261 agatttttcc gatgtgaagg ggcacgaaca ggccaagcgc gccatcgagg tggcggcggc
     1321 ggggggccac aatctgctga tgatcgggtc gccggggtcg ggcaagacga tgctcgcgcg
     1381 ccgcatcccg tcggtgttgc cgctcttgga agaagaggaa cggatagaga ccaccaagat
     1441 attcagcgtc gcgggactgc tcgcggagga ggggctcatc gtcgagcggc cgttccgcag
     1501 tccgcaccat acgctgtcgg atgtcgcgct cgcggggggc ggcgtgccgc cgcgaccggg
     1561 ggagatatcg ctcgcccaca acggggtgct gttcctcgac gagttccccg aattcaagaa
     1621 gaacgcgctc gaggtcctgc gccagccgct cgaggacggt gagatcacga tatcgcgcgc
     1681 cacctcgacg gtgaaattcc cgtcgagttt catgctcgtc gcctcgatga acccgtgccc
     1741 gtgcgggtat ctgttcgacc gcaagcaccc ctgcacctgt tcgcaggtcg ccatacagaa
     1801 ataccagcac aagatatcgg gtccgctcct cgaccgcatc gacatatcgg tcatcgtcaa
     1861 ctcggtcccg tacgaggacc tgcgcaaaaa agggggcggc gaaccgtcgg ataagttgcg
     1921 cgaccgcatc gagagggccc gcacggtgca ggcg
//
LOCUS       DAOQUE010000511         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_76216_length_1937_cov_3.914984, whole genome shotgun sequence.
ACCESSION   DAOQUE010000511 DAOQUE010000000
VERSION     DAOQUE010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76216_length_1937_cov_3.914984"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1937)
                     /locus_tag="PKH10_12470"
     CDS             complement(<1..>1937)
                     /locus_tag="PKH10_12470"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNT28977.1"
                     /translation="ARVTGQLEHPGIVPVYEIGRRPDGAPYYTMRLVRGETLSDAIGA
                     AKTLRDRLRLLPHFRDICNAVAYAHSRGVVHRDIKPDNIMIGQFGETVVLDWGLAKVR
                     GLSDDRADEMAQGISALRETPVRRTLTGRAFGTPTYMPPEQARGLVAEVDERSDIYAL
                     GAVLYEILAGAPPFDERTATEIIAKVLSERPIPIASLERQAPPDLCAVVEKALSKKKE
                     ERYGSAREMAREIENYMAGGRVEVYEYSSWELLLRFVRKNRALSLLIGVLLVLIVLGV
                     SAVFSAWRNAVENERIAHLNLALGYQESADRMLQERRYDKAEIFAAASLGHNPWNPRS
                     PYRFPDIAARSSGDIARNTLSARSSLFLARMNQNDALVAVAPYRGSEVRDMDMSPDGK
                     VVALAGKDGIITLWGIEEQDEIDRLPGHRDEVTRVVFSPDGKMLASASWDRSVRLWDV
                     ATRTELATLGGHTEEVYAIAFSSDGRVIVSGGNDGTVRRWDVAARREISLRSLPDAKI
                     RSIAVSPDGASIAVGTASGELYLVQDRAMASFRLHTEAIVALRYRSDGKRLYTAGYDK
                     KALVVDPATGRVLDSFIYWDAFFALSIDRDGSRSVIASRDGTLLLRDMTTGRTDQLRG
                     HDLSAYAVAFTPAGDRLVSAG"
BASE COUNT          355 a          650 c          598 g          334 t
ORIGIN      
        1 ccctgccgac acaagacgat cgccggcggg agtgaaggcg accgcatagg cggaaagatc
       61 gtgcccccga agttgatcgg tccggccggt cgtcatgtcg cgtagcagga gggtgccatc
      121 gcgcgaggcg atcaccgaac gggaaccgtc ccgatcgatg ctcagtgcga aaaaggcatc
      181 ccagtagatg aaagagtcga gcacccgacc ggtcgcggga tcgaccacca acgccttttt
      241 gtcataaccc gccgtataca gtcgtttccc gtcggaccgg taacgcaggg cgacgatcgc
      301 ctcggtatgc aggcggaagg aagccatggc gcggtcttgc accagatata gttcccccga
      361 tgcggtaccc accgcgatcg acgcaccgtc gggcgacaca gcgatcgacc gtatcttcgc
      421 atcaggcagg gaacgcagcg atatctcacg gcgtgcggcg acatcccagc ggcgcaccgt
      481 accatcgttg cctcccgaca cgatgacgcg accgtcggac gagaaggcga tggcatacac
      541 ctcctcggtg tggccgccga gcgtagcgag ttcggtccgg gtcgcgacat cccagaggcg
      601 caccgatcgg tcccagctgg ctgaggcaag catcttcccg tccggcgaga acacgacacg
      661 cgtcacctcg tcacgatggc cgggaagccg gtcgatctcg tcctgctctt ctatgcccca
      721 gagagtgatg atgccgtcct tcccggcgag cgcgacgacc ttgccatccg gagacatatc
      781 catatcgcgc acctcgctcc cccgatacgg ggccaccgcg acgagcgcgt cgttctggtt
      841 catccgggca aggaacagac tcgaccgggc cgacagcgtg ttgcgcgcta tatcgccgga
      901 cgatcgcgca gcgatgtcgg ggaaccggta cgggctccgc ggattccacg ggttgtgtcc
      961 gagcgacgcg gcggcgaata tctcggcctt gtcataccgc cgttcctgta gcatgcgatc
     1021 ggcactctcc tgatacccga gcgcgagatt aagatgggcg atccgttcgt tctcgacggc
     1081 attccgccac gcggagaaga cggcggacac accgagcacg atgaggacga gcagaacgcc
     1141 gatgagcagg gagagcgccc ggttcttgcg gacgaaccgc agcagaagtt cccacgaact
     1201 gtattcgtac acctcgacac gaccgcctgc catgtagttc tctatctcgc gcgccatctc
     1261 acgcgccgaa ccgtagcgtt cctctttttt ctttgacagc gccttttcca cgacggcgca
     1321 gagatcgggc ggggcctgcc gctcgagcga tgcgatgggt atcggcctct cgctcaggac
     1381 ctttgcgatg atctcggtcg ccgtgcgctc gtcgaatggt ggcgcgcccg cgagtatctc
     1441 gtacagcacc gcgccgagcg catagatgtc gctccgctcg tccacttcgg cgaccagtcc
     1501 gcgcgcctgt tccggcggca tataggtggg ggtcccgaac gcgcgtccgg tcagcgtccg
     1561 gcgcaccggc gtctcccgga gcgccgatat gccctgcgcc atctcgtcgg cacggtcgtc
     1621 cgaaagtccg cgcaccttgg cgagccccca atcaagcacc accgtctcgc cgaactgccc
     1681 tatcatgatg ttgtccggtt tgatgtctcg atgcaccaca ccgcggctgt gcgcgtaggc
     1741 gacggcattg cagatgtccc ggaaatgagg caggagccgg agccggtcgc gcagcgtctt
     1801 ggccgctccg atggcatctg acagcgtttc gccgcgcacc aatcgcatgg tatagtaggg
     1861 agcaccgtcc ggcctccgac ctatctcgta gaccggcacg ataccggggt gctccaactg
     1921 gccggtcacc cgcgcca
//
LOCUS       DAOQUE010000512         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_76798_length_1928_cov_3.525360, whole genome shotgun sequence.
ACCESSION   DAOQUE010000512 DAOQUE010000000
VERSION     DAOQUE010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76798_length_1928_cov_3.525360"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PKH10_12475"
     CDS             <1..458
                     /locus_tag="PKH10_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28978.1"
                     /translation="PSKEELIRTVVLSIMLPKMARMKGLMQGSGSVADFFGGVIEVFH
                     SLGREVSEPMMLDMKMSPELWKEIEERRLEVLSHIGEVIERGKKSGEVRADLNVDLFL
                     RIFMLVINRIGNPTMMLELNMKPSDLAGQLFGIFFHGIVPADRRAGGVS"
     gene            455..1429
                     /locus_tag="PKH10_12480"
     CDS             455..1429
                     /locus_tag="PKH10_12480"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT28979.1"
                     /translation="MKKMFAVALFLLLAACNGKENKNEIVLTGSVESDTVRISSLVPG
                     RLTEVNAVEGAKVKKGAVLARIDTADLELQLKQADTGVRAAAARLALVKKGARSEDLA
                     TAKELVNQAEIAADKFEREYQRLSRLKEAGSVTEKDLDDMMTQRDRAKSQVEQAKKQY
                     EKAQNGAQKEEIDAAAAAYDQARSAIDILKKRIADCTVYAPRDGTVLHRLAEPGEVIG
                     AGSSIAVISDLATVKITAFVPERDLGLVKLGTPATVRVDSFPDKPLSATVSRIADVAE
                     FTPKTIQTQEERVKTVYRIELTADNKEGVFKPGMPVDVVFERPPVKGE"
     gene            1500..>1928
                     /locus_tag="PKH10_12485"
     CDS             1500..>1928
                     /locus_tag="PKH10_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT28980.1"
                     /translation="MTDLVLQAENLHFSFGKVPALAGVDLTVEKGEIFGIVGPDGAGK
                     TTLIRIFCGLLPPAAGSCRVLGFDTVREKMALIRKIGYLSQRFSLYGDLTVSENIDFF
                     AEIHGVKDFAARKKELLDYMDLERFRGRLSGALSGGMKQKL"
BASE COUNT          405 a          564 c          629 g          330 t
ORIGIN      
        1 tcccctcgaa ggaagaactc atccgcaccg tcgtcctctc catcatgcta ccaaaaatgg
       61 cacgaatgaa agggctcatg cagggatcgg gcagcgtcgc cgacttcttc ggcggcgtca
      121 tcgaggtgtt ccactcactc ggccgcgagg tgtcggaacc gatgatgctc gacatgaaga
      181 tgtcgcccga actctggaag gagatcgagg agcggcggct cgaggtcctt tcgcacatcg
      241 gcgaggtgat cgagcgcggc aagaagagcg gcgaggtacg cgccgatctc aacgtcgacc
      301 tcttcctgcg catcttcatg cttgtcatca accgtatcgg caatccgacg atgatgctcg
      361 aactcaacat gaaaccatcc gatctcgccg ggcagttatt cgggatattc ttccacggca
      421 tcgtgccggc cgaccggcgc gcgggaggtg tgtcatgaaa aagatgttcg cggtagcgct
      481 tttccttctt ctcgccgcct gcaacgggaa agagaacaag aacgagatcg tccttaccgg
      541 ttcggtcgaa agcgacacgg tgcggatatc ctcgctggta ccggggcggc tgaccgaggt
      601 gaacgccgtc gaaggggcga aggtgaagaa gggggcggtc ctcgcgcgga tcgacaccgc
      661 cgatctcgaa ctgcaactga agcaggccga caccggcgtg cgagccgccg cggcgcggct
      721 ggcgctcgtc aaaaaagggg cccgtagcga ggatctggcg accgccaagg agttggtcaa
      781 tcaggccgag atcgcggccg ataagttcga acgcgagtat cagcggctgt cgcggctcaa
      841 ggaggcggga tcggtcaccg agaaggatct cgacgacatg atgacccagc gcgaccgagc
      901 aaaaagtcag gtcgaacagg ccaaaaagca gtacgagaag gcgcagaacg gggcgcagaa
      961 ggaggagata gacgcggcgg cggcggccta cgatcaggcc cgttcggcca tcgacatcct
     1021 gaaaaagagg atcgccgact gcaccgtcta tgcgccgcgc gacggcacgg tgctccaccg
     1081 gctcgccgaa ccgggagagg tgatcggtgc ggggagcagc atcgccgtca tctccgatct
     1141 cgcgacggtc aagataacgg ccttcgtccc ggaacgcgac ctcggcctcg tgaaactcgg
     1201 gacaccggcg acggtgcggg tcgattcgtt ccccgacaag ccgctttcgg cgacggtgtc
     1261 gcgcatcgcc gatgtcgccg aattcacccc gaaaacgata cagacgcagg aggagcgggt
     1321 caaaacggtc tatcggatcg agctgaccgc cgacaacaaa gagggcgtct tcaagccggg
     1381 gatgccggtc gatgtggtgt tcgagcgtcc ccctgtcaag ggggaatgag ggggttttat
     1441 gccgtatcga tgcggaaaac cctccctgcc ctcccttgac agggcgggat ggaggaggca
     1501 tgactgattt agtcctccaa gctgaaaatc tccatttctc gttcgggaag gtaccggcgc
     1561 ttgccggtgt cgacctgacg gtggagaaag gggagatctt cggcatcgtc ggtcccgacg
     1621 gcgccgggaa gacgacgctc atccgcatct tctgcgggct tctgcctccc gccgccgggt
     1681 cgtgccgcgt gctcgggttc gacacggtga gggagaaaat ggcgctcatc cgcaagatcg
     1741 gctacctttc gcagcgcttt tcgctctacg gcgatctcac ggtgtcggag aacatcgact
     1801 tcttcgccga gatacacggc gtgaaggatt tcgcggcgcg caagaaggaa ctgctcgact
     1861 acatggatct cgaacggttc cgcggccgcc tctcgggggc tctgtcgggc ggcatgaagc
     1921 agaaactg
//
LOCUS       DAOQUE010000513         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_76850_length_1927_cov_4.641026, whole genome shotgun sequence.
ACCESSION   DAOQUE010000513 DAOQUE010000000
VERSION     DAOQUE010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76850_length_1927_cov_4.641026"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PKH10_12490"
     CDS             <1..1241
                     /locus_tag="PKH10_12490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT28981.1"
                     /translation="PIRNEEGEMIGVVLVFRDSTLKKNLEEQTRRMQRLESLGLLAGG
                     IAHDFNNYLAGTLGSISVARMLLPDDHKAQEMLQLAERSSHRAQHLTTQLLTFSKGGH
                     PVRRTTDLLATLRESVGFSSAGSSIKVTVLVDGDIAPVWADEGQILQLLNNLLLNAVQ
                     AMPSGGAVTVRVGQATVDRSAGVPCPPGDYVTVAVSDTGKGIAPEHLPHIFDPYFTTK
                     TSGTGLGLTVAHSIATRHDGCITVSSQSGHGSTFTLYLPVSPEKRPSTTAAESKPHSN
                     GGRILIMDDEEVIREMLASALRFLGYDPVTVATGDEAIVRHREEKQGRTPFTALILDL
                     TIPGGKGGEETLRTIRATDPAVRAIAMSGYADSPILDRPLEYGFRAAIAKPFTVDDIG
                     RTLEKVLTVSPSDTSSNAPA"
     gene            complement(1198..>1927)
                     /locus_tag="PKH10_12495"
     CDS             complement(1198..>1927)
                     /locus_tag="PKH10_12495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HNT28982.1"
                     /translation="IRHVVLCALCASLGGRERPFDIVSEREFQPALLAPTVPVTTDDE
                     RAGRFMERIATVMGRRVRRTLVRAFLSDLPGREMLLYDYLAFLRDRGPRGDACLTHPA
                     VAPVRDLARQVTVEAHRLTGLVRFTRVGDGLLLAVMEPRHLVLPLIAPHFTRRFPAER
                     WVMLDVGRGVGLYWDTKDLHEVESGPEVTRLLNRPPDDPQGDLYEKMWRRYFRDIAIP
                     ERRNPHCQRRFMPVRYWKYLTEKR"
BASE COUNT          387 a          646 c          585 g          309 t
ORIGIN      
        1 cgccgatacg gaacgaggag ggcgagatga tcggtgtcgt gctcgtgttc cgcgacagca
       61 ccctgaaaaa gaacctcgag gagcagaccc gacgcatgca acggctcgag tccctcggcc
      121 tgctcgccgg cggcatcgcc cacgatttca acaactatct cgccggtacg ctcgggagca
      181 tcagcgtcgc ccgcatgcta ctgcctgacg accacaaggc tcaggagatg ctccaactcg
      241 ctgagaggtc aagccaccgc gctcaacacc tcacaacgca actgctcacc ttctccaaag
      301 gcggccatcc ggtgcgccgt acgaccgacc tcctcgcgac acttcgcgaa agcgtcggat
      361 tctcgtcagc cggatcatcg atcaaggtga cggtcctcgt cgacggtgac atcgccccgg
      421 tatgggctga cgagggacag atactgcaac tgctcaacaa cctccttctg aacgcggtac
      481 aggctatgcc gagcggcggc gccgtcaccg tgcgggtcgg ccaagcgacc gtcgatcgct
      541 cagccggtgt cccctgcccg cccggcgatt atgtcacggt cgccgtcagc gacaccggca
      601 aagggatagc accggaacat ctaccccata ttttcgaccc ctacttcacc accaagacga
      661 gcggcaccgg actcggactc accgtcgctc actccatcgc gacgcgtcac gacgggtgca
      721 tcactgtttc ctcgcagagc ggccacggat cgaccttcac tctctatcta ccggtatcgc
      781 ccgaaaaacg gccgtcaacg accgcagcgg agtcaaaacc acattcgaac ggcgggcgca
      841 tcctcatcat ggatgacgaa gaggtgatcc gagaaatgct tgcgtcggcg ctccgctttc
      901 tcggctacga cccggtgacg gtcgcgaccg gcgacgaggc gatcgtccgc catcgtgagg
      961 aaaaacaggg aagaactcct ttcacagcgc tcatcctcga tcttactata ccgggcggta
     1021 aaggtggcga ggagacgttg aggaccatcc gtgcgaccga tccggcggtg cgcgccatcg
     1081 ccatgagcgg ctatgccgat tcccccatcc tcgatcgacc gctggagtac gggttccggg
     1141 cggccatcgc aaaacccttt accgtcgacg acatcgggcg gacactggaa aaggtgctta
     1201 ccgtttctcc gtcagatact tccagtaacg caccggcatg aatcgccgct gacagtgcgg
     1261 gttgcgccgt tccggtatcg cgatgtccct gaaatagcgt cgccacatct tctcgtagag
     1321 gtcgccctgc gggtcatcgg gcggcctgtt gaggagccgc gtcacctcgg ggccgctttc
     1381 gacctcatgc agatccttcg tgtcccagta aagcccgacg ccgcgcccga catcgagcat
     1441 gacccatcgc tcggcaggga aacggcgggt gaagtgcggc gcgatgagcg gcaggaccag
     1501 atggcgcggc tccatgaccg caaggaggag tccgtcaccg acgcgggtga agcggacaag
     1561 accggtgagc cggtgcgcct cgaccgtcac ctgccgcgca aggtcgcgga ccggcgcaac
     1621 ggcgggatgg gtaaggcagg cgtcgccccg cgggccgcgg tcgcgcagga aggcaagata
     1681 gtcgtagagg agcatttcgc gccccggcag atcggaaaga aaggcgcgga caagagtgcg
     1741 ccggacacgg cgccccatga ccgtcgctat ccgttccatg aagcgtccgg cacgctcatc
     1801 gtcggtcgtc accggaacgg tcggcgcaag gagggccggc tggaactccc gttcgctcac
     1861 gatgtcgaac ggccgctctc tcccgcccag cgacgcgcag agggcgcaga ggaccacgtg
     1921 ccttatc
//
LOCUS       DAOQUE010000514         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_77156_length_1922_cov_5.165506, whole genome shotgun sequence.
ACCESSION   DAOQUE010000514 DAOQUE010000000
VERSION     DAOQUE010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77156_length_1922_cov_5.165506"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PKH10_12500"
     CDS             complement(<1..742)
                     /locus_tag="PKH10_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT28983.1"
                     /translation="MRIKNISLRNFRKFTEISFDLSTRVVILGPNGSGKTTLAEAGAL
                     VLGGRSFRTADLKECIREGAPYAFVKAVLLLDDITENAVTVGMDRKGQRKILRDERSV
                     TRRSLVAAHPFVVCTQEDIDMVAGSPKRRRDFLDKIAMGHDRDYIDILTPYLRFVKQK
                     SALLKSGRSGGLTHLNEAAVPLIGEIRRRRRMAADAVTARFAGVADRASVDIAAEIRL
                     PSETDDGEIRARLRERAAREKERGCLLYG"
     gene            complement(755..1897)
                     /gene="dnaN"
                     /locus_tag="PKH10_12505"
     CDS             complement(755..1897)
                     /gene="dnaN"
                     /locus_tag="PKH10_12505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNT28984.1"
                     /translation="MSKIIFKRDDILKAVEYCMNSVEKKSTVPMLSQILLDVSKKKGT
                     LTSTNLDTSTIAQITPIECDEGVKITIPARYFYEICKGVRGDTIKIEYQSEHNIISVT
                     SDKTNYNLPCGDVKDFPLVSAEVGEEVLIPIKRMIPFFKKLQFSMTDPNYNKVYSGVL
                     LTQVTEEKKEVFEMVTTDIHRISIVTLDNFKLPVKEFSEGIVVPGKNFSEIVKIFSDV
                     EKAHISIKEGKLFLRSDTVTFICRLIKNEFPNYRNVTGSPSQIEKKEYADINRRELIE
                     GIKRVIALSTEEKIWAARFEFKGDALRMSASSEFGGSSSDEILVEKGFKGERAIGLNA
                     RYFLDVVSVIDDEKVRLVVEEGLRPLTVVEKGADFTYTHMVMPLRI"
BASE COUNT          390 a          543 c          498 g          491 t
ORIGIN      
        1 gcccgtagag gagacacccc cgctcctttt cccgggcggc ccgctcccgg agccgggccc
       61 ggatttctcc gtcatcggtc tccgagggaa gccgtatctc cgccgcgata tcgaccgacg
      121 cccgatcggc gaccccggcg aaccgggccg tcaccgcatc ggccgccatc cggcgacggc
      181 ggcggatctc gcctatgagc ggcaccgccg cctcgttgag atgcgtaagc ccgccggacc
      241 tcccgctttt caacaaagcg ctcttctgct tcacgaaacg gagatagggg gtgaggatgt
      301 cgatgtagtc gcggtcgtgc cccatggcga tcttgtcgag aaaatcgcga cggcgtttcg
      361 gggacccggc caccatgtca atgtcctcct gcgtgcaaac gacgaaggga tgggcggcga
      421 cgagcgaccg acgggtgacc gatcgttcgt cgcggagtat cttccgttgt cctttgcgat
      481 ccattccgac agtgaccgcg ttctcggtta tatcgtcaag cagcaggacc gctttgacaa
      541 aagcatacgg agcgccctct cggatgcact ccttcaggtc ggcggtgcgg aaacttcttc
      601 cgcccagcac gagagcgccg gcctcggcga gagtggtctt gcccgacccg ttgggtccca
      661 gtatgaccac gcgtgtcgaa aggtcaaacg aaatttcggt gaacttcctg aaattcctta
      721 gagatatgtt ctttatgcgc atccggcggc actgctatat ccggagcggc atgaccatgt
      781 gggtataggt gaaatcggcc cccttctcga cgaccgtcag gggacgcagg ccctcttcga
      841 ccacgagacg caccttctcg tcgtctatca cgctgaccac atcaaggaaa tagcgcgcgt
      901 tcagtccgat ggcccgctcg cccttgaacc ctttttcgac cagtatctcg tcgctcgaac
      961 tgccgccgaa ctcggaactc gcgctcatcc ggagagcatc ccctttgaat tcgaaccggg
     1021 cggcccatat cttttcctcg gtcgaaaggg ctatgacgcg cttgatacct tcaatgagtt
     1081 cgcgccggtt gatgtcggcg tattcctttt tctctatctg tgaggggctt cccgtcacat
     1141 tccggtagtt cgggaactcg ttcttgatga gacggcagat gaaggtgacc gtatcggaac
     1201 gaaggaataa tttcccctct ttgatggaga tatgcgcttt ttccacatcg gagaatatct
     1261 tgacgatctc ggaaaaattc tttcccggca ccacgatacc ttccgagaat tccttcactg
     1321 gcagtttgaa gttatcaagc gtcacgatcg atatccggtg aatgtcggtc gtgaccattt
     1381 cgaacacttc ctttttttct tcggtcacct gcgtcagaag aacgccggaa tagaccttat
     1441 tgtagttcgg atcagtcata gagaactgca gttttttaaa gaacgggatc atcctcttta
     1501 tcgggatgag tacctcttcc cctacttcgg ccgatactaa tggaaaatct ttaacatcgc
     1561 cgcagggcag attatagttc gttttgtcgg aggtcaccga tatgatattg tgttcgcttt
     1621 gatattctat ttttatagta tcccctcgta ctcctttaca tatttcatag aaataccggg
     1681 cgggtatggt tatttttacc ccttcatcgc attcaatggg ggttatctgt gcgatggtgg
     1741 aggtgtcgag attcgtcgag gtcaatgtcc cttttttctt cgatacatcg agtagtatct
     1801 gcgacaacat aggaacggtg ctcttctttt cgaccgaatt catacaatac tcgaccgctt
     1861 taagaatgtc gtcccgttta aagatgatct tcgacatggt ggccctccat acggtttatt
     1921 at
//
LOCUS       DAOQUE010000515         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_77284_length_1920_cov_3.921180, whole genome shotgun sequence.
ACCESSION   DAOQUE010000515 DAOQUE010000000
VERSION     DAOQUE010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77284_length_1920_cov_3.921180"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PKH10_12510"
     CDS             <1..1121
                     /locus_tag="PKH10_12510"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28985.1"
                     /translation="ETDRFVGEGGILMLADGYLYSAEGSDLAVYDLADPLHPERIAWR
                     AIGDMKAFGVFVQDGAAYLRGIDIGLSVVDVSAPGQLPILFSNPSPSDHAEAVALSGN
                     HAYLGDRAFGRGIAQIDVTAPGLPFVIDAINFSGAGQDVFISGNDLFVAAFYNLMIVD
                     ITDPLAIPTPRFVDTDGIASGAHVQNGFAYVASWENGLAVVDLTPGTPVVVGTVDTPG
                     KAAEVFAAGDHAYVADGETGLQVIDVSDPAAPAIIATIDTPGEALGLFIEGSYAYVAD
                     GPYFLVVDISDPTTPAVIAQRAVGGYAQDVFLQGSLAYVAAYGGGLALFDIADPAVPE
                     LVGQIPVYGEPTGVVVRDDLVFLSEHNGAFEIVRLTCE"
     gene            1198..1407
                     /locus_tag="PKH10_12515"
     CDS             1198..1407
                     /locus_tag="PKH10_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28986.1"
                     /translation="MEQKRPVQSELQSKTLIINEVDGIDDMLSDLNKGRGQLEKKERS
                     FDWRTALIVLLVIVVLAETVWLLIR"
     gene            1389..>1920
                     /locus_tag="PKH10_12520"
     CDS             1389..>1920
                     /locus_tag="PKH10_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28987.1"
                     /translation="MASHPLRERLSRIRRPFQRAFFAALFAGAAFFFVKHGLIVLRTD
                     ICDNNFHATAKVITLAAAGWVGMVLALVGFLSIWRRSRIFITRRAYLAAILLLLPLMF
                     YLWDFTVQHSLKRYPVSGDEISMIFQADVFSHGMLWNRTMANEIAPHFKRHHVITTPE
                     REYSKYPPGMPLLMALF"
BASE COUNT          334 a          644 c          548 g          394 t
ORIGIN      
        1 cggagaccga tcgtttcgta ggcgaagggg ggatacttat gctcgccgac ggctatcttt
       61 acagtgccga aggttcggat ctcgccgtct atgacctcgc cgacccgctc catcccgaac
      121 ggatcgcgtg gcgggccatc ggcgacatga aggcgttcgg cgtgttcgta caggatggcg
      181 ccgcctacct ccggggcatc gacatcggcc tgagcgttgt ggatgtctcg gcccccggcc
      241 aacttccgat ccttttcagc aatccgtcgc catccgacca cgccgaggcg gtcgcgctct
      301 cggggaacca tgcgtatctc ggggatcggg ccttcggcag gggcatcgcc cagatagatg
      361 tcacggcgcc cggactgccc ttcgtcatcg atgcgatcaa cttctccggc gcagggcagg
      421 atgtgttcat ctcggggaac gatctgttcg tcgcggcctt ttacaacctc atgatcgtcg
      481 acatcaccga tccactcgcc atacccacgc cccgtttcgt ggacaccgac ggcatcgcga
      541 gcggcgcaca tgtgcagaac ggtttcgctt atgtggcctc atgggagaac gggctggcgg
      601 tcgtcgacct gaccccgggc actcccgtcg tcgtcggcac ggtcgatacg ccgggcaagg
      661 ccgccgaggt cttcgccgct ggagatcatg cgtatgtcgc cgacggtgag acgggcctgc
      721 aggtgatcga tgtctccgac cccgccgcgc ctgccatcat cgccacgatc gacacgccgg
      781 gcgaggcact cggcctgttc atcgaaggat cgtacgccta tgtcgctgac ggcccctatt
      841 tcctcgtcgt cgatatctcc gatccgacga ccccggcggt catcgcccag cgagcggtcg
      901 gcggctacgc gcaggatgtc tttctgcaag gatcgctggc ctatgtggcc gcgtatggcg
      961 gcgggctcgc cctgttcgac atcgccgatc ccgccgtacc ggaactggtc gggcagatac
     1021 cggtgtacgg tgaaccgacc ggcgtggtcg tccgcgacga cctcgtgttc ctctccgagc
     1081 acaacggcgc cttcgagatc gtccgcctga cctgcgagtg accccgccga aaattgactt
     1141 taccctcgtt ataccataga gttcgcgcgt taccttcttt aaggagcaga gacctccatg
     1201 gagcagaaaa gacctgtcca gagcgaactg cagagcaaaa cgctcatcat caacgaggtc
     1261 gacggcatcg acgacatgct gagcgacctc aacaagggcc gcggtcaact tgagaagaag
     1321 gaacgctcgt tcgactggcg gaccgctctc atcgtactac tcgtcatcgt cgtactggcc
     1381 gagacggtat ggcttctcat ccgctgaggg aacgcctttc ccgcatacgc cgccctttcc
     1441 agcgcgcttt tttcgcggcg ctcttcgccg gcgcggcctt tttcttcgtc aaacacggcc
     1501 tgatcgtcct gcggaccgat atctgcgaca ataattttca cgcgaccgcg aaggtcatca
     1561 ccctcgcggc ggccggctgg gtcggtatgg tccttgcgct ggtcggcttt ctgtcgatct
     1621 ggcgccgatc acgcatcttc atcacgcgac gcgcctatct ggcggccatc ctcctgctcc
     1681 tcccgctcat gttctacctc tgggacttca ccgtacagca ttcactcaag cgctaccctg
     1741 tgtcgggcga cgagatatcg atgatattcc aagccgatgt cttttcgcac ggcatgcttt
     1801 ggaaccgcac gatggccaac gagatcgccc cccacttcaa gcggcaccat gtcatcacga
     1861 cgccggagcg ggaatactcg aaataccccc ccggcatgcc gctcctcatg gcactcttca
//
LOCUS       DAOQUE010000516         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_77363_length_1919_cov_3.315987, whole genome shotgun sequence.
ACCESSION   DAOQUE010000516 DAOQUE010000000
VERSION     DAOQUE010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77363_length_1919_cov_3.315987"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKH10_12525"
     CDS             complement(<1..265)
                     /locus_tag="PKH10_12525"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNT28988.1"
                     /translation="MRTFHIHTFGCQMNVYDSERVGALLTARGWVPVDDPGQAQLLIV
                     NTCSVREKPAQKVFSFVGRFLAEKERGAMSIFVMGCVAQQLGAD"
     gene            complement(288..>1919)
                     /locus_tag="PKH10_12530"
     CDS             complement(288..>1919)
                     /locus_tag="PKH10_12530"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNT28989.1"
                     /translation="EDFVDDFCIVNGDSVELSIFSTDDLSRPYVFDETRVLPVGGLTG
                     SSDFSSLRSNRYYRFFVKVTNTQEKVKLTGGAEGILYEDTKNYEINIFLGLTGDFVRV
                     VSEQSDKTSLSTYFTEDDVPSGSRAVVLKDGRIVLTGGSIWRDSGSESYLTRTNYIDM
                     AKLTVTDGPSLRMGVKDHVAALLETTDAKGALSPKTGKVVIAFGKTQDGYNDKIYLLD
                     PEKNTVEQKVSFTNRAHARALTIGGEVYISGGCDSGSASRDIIKVDKKGNASSWKLME
                     QGRCLHGMIDVSWVTTDDEGVETLHPAILVFGGAKKYINTDQDKELIGYSDEAFAEVI
                     DEGGTQKITVAGVECVTTGAQTSCTSLAGFGAARVKWDDPNKGPNEDIVVAASGGFTQ
                     TANDQPLSFSIANYILRGTGDLTNPKGMTWALSSSGTAIRCAYATMVPISSPDSFTVQ
                     YTALNCGTGKSIERNHDARNEQYIYSLEVRAAGTPGDFDLSASARASVNDGSIDNSNS
                     GYFLDGPTVVNDFGQAFLFGTRYIYVISGFSDSLD"
BASE COUNT          352 a          576 c          596 g          395 t
ORIGIN      
        1 ggtccgcccc caactgctgg gcgacgcacc ccatcacgaa gatgctcatg gccccccgct
       61 ccttttccgc gaggaaccgg ccgacgaaac tgaacacctt ctgcgccggt ttctcccgca
      121 cgctacaggt gttgacgatg agcaattgcg cctgccccgg atcgtcgacc ggcacccatc
      181 cgcgtgccgt cagaagcgcg ccgacccgtt cggagtcgta gacattcatc tggcatccga
      241 aggtatggat gtgaaaggtc ctcaccggaa cggagagatg gctgggatca gtcgagactg
      301 tcggagaatc cgctgatgac atagatgtac cgggtcccga acaggaaggc ctgaccgaag
      361 tcgttgacca cggtggggcc atcgaggaag taaccggagt tgctgttgtc tatggagccg
      421 tcgttgacgc tggcgcgcgc cgaggccgaa agatcgaagt caccgggggt accggcggca
      481 cggacctcga ggctgtatat gtactgttcg ttccgcgcgt catgattgcg ttctatcgat
      541 ttaccggtgc cgcagttgag tgcggtgtac tgcaccgtga aactgtcggg ggaactgatg
      601 gggaccatcg tcgcgtaggc gcaccgtatc gccgtaccgg acgaagagag cgcccaggtc
      661 atccccttgg ggttggtcag gtcaccggtg ccccgcagga tgtagttggc gatgctgaag
      721 gaaagcggct ggtcgttcgc ggtctgcgtg aaaccgcccg acgccgcgac cacgatatcc
      781 tcgttcggac ccttgttcgg atcgtcccac ttcacccgcg ccgcaccgaa accggccagc
      841 gaggtgcagg aggtctgcgc gccggtggtg acgcactcga cgcccgcgac cgtgatcttc
      901 tgcgtgccgc cctcgtcgat gacctcggcg aacgcttcgt cgctgtaccc gatcagttcc
      961 ttgtcctgat cggtgttgat gtatttcttc gcgccgccga acacgagtat ggcgggatgc
     1021 agggtctcca cgccctcatc gtcggtcgtg acccacgaaa catctatcat gccgtggagg
     1081 cagcgaccct gttccatcag tttccacgaa gaggcgttgc ccttcttgtc gaccttgatg
     1141 atgtcgcgcg aagccgagcc cgaatcgcac ccgccgctga tgtatacctc gccgccgatg
     1201 gtgagggcgc gggcatgggc gcggttggtg aacgacactt tctgttcgac cgtgttcttt
     1261 tcgggatcga ggaggtatat cttgtcgttg tagccgtcct gtgtcttgcc gaacgcgatg
     1321 accaccttgc cggtcttcgg cgaaagagct cctttggcat cggtcgtctc gagaagcgcc
     1381 gcgacatgat ccttgacgcc catcctgagc gagggaccgt cggtcacggt cagtttcgcc
     1441 atgtcgatgt aattcgtcct cgtcaggtag gattctgagc cgctgtcgcg ccagatcgaa
     1501 ccgccggtga gcacgatgcg accgtccttc aggacgactg cgcgggaccc ggagggaaca
     1561 tcgtcctcgg tgaagtaggt cgaaagcgag gtcttatcgc tctgttcgga cacgacgcgg
     1621 acgaaatcgc cggtaagacc gaggaagatg tttatctcat agttcttggt gtcctcgtac
     1681 aggatgccct cggcgccgcc ggtcaacttc accttttcct gcgtgttggt caccttcacg
     1741 aagaaccggt agtagcggtt gctccggagg gaactgaagt cggaactgcc cgtcaggccg
     1801 cccaccggaa gcacgcgggt ctcgtcgaag acataggggc gcgacaggtc gtcggtcgag
     1861 aatatcgaga gttccaccga atcgccgttc acgatgcaga agtcgtcgac gaaatcctc
//
LOCUS       DAOQUE010000517         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_77546_length_1916_cov_3.993015, whole genome shotgun sequence.
ACCESSION   DAOQUE010000517 DAOQUE010000000
VERSION     DAOQUE010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77546_length_1916_cov_3.993015"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PKH10_12535"
     CDS             <1..631
                     /locus_tag="PKH10_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HNT28990.1"
                     /translation="GKELNKIEVLALLTKLRLAANHPMLVSDRVRDIEDSGKIALLME
                     LIGDIRDGGGRVLVFSQFVKMLAIVEKALKKRAIPYFYMDGDTVDRRDPVDRFNRGER
                     EVFLLSLKVGGFGLNLTGADHVVIVDPWWNPAVEEQAWSRAHRIGQQRQVVVSKIFSR
                     GTIEEKILDLQQNKKDLSGFFLSRSMVEPSKDLIRLLAEMELRSPGEGA"
     gene            749..1069
                     /gene="rplU"
                     /locus_tag="PKH10_12540"
     CDS             749..1069
                     /gene="rplU"
                     /locus_tag="PKH10_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNT28991.1"
                     /translation="MYAVVNFGGHQVKAVPGTKIFVNALHREKGEVFENTEVLLVADG
                     DRIEVGKPHVAGATVKFEVIDTIKGDKVIVFKKKRRKGYRRKRSHRALATALKVVEIQ
                     QKKK"
     gene            1081..1359
                     /gene="rpmA"
                     /locus_tag="PKH10_12545"
     CDS             1081..1359
                     /gene="rpmA"
                     /locus_tag="PKH10_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNT28992.1"
                     /translation="MAHKKSGGSARNGRDSQGQRRGIKIYGGETVRAGNILVRQCGTK
                     WHAGENVGTGSDYTLFALIDGVVKYQTYRRRGTEKTRVHVVPAGTPEK"
     gene            1415..>1916
                     /locus_tag="PKH10_12550"
     CDS             1415..>1916
                     /locus_tag="PKH10_12550"
                     /inference="COORDINATES: protein motif:HMM:NF013205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNT28993.1"
                     /translation="MTVVKFVDEAIVKVRAGNGGKGAVSFLREKYMPLGGPDGGDGGK
                     GGDVVIEADENVGTLYDVRFKREITAESGAAGMGKNCFGRSGKDTIVRVPVGTVIYEE
                     GREEPLGDLVRHGQRVIVARGGRGGQGNARFKTARRQAPKFAQPGEEGKDTIVRVPVG
                     TVIYEEG"
BASE COUNT          415 a          525 c          652 g          324 t
ORIGIN      
        1 aggcaaggag ctcaataaga tagaggtgct tgcgctcctc accaaactgc ggcttgcggc
       61 caaccatccg atgctcgtgt cggaccgggt gcgtgacatc gaggattcgg gcaagatagc
      121 gctccttatg gaactcatcg gcgatattcg cgacggtggc ggacgggtgc tcgtgttcag
      181 ccagttcgtc aagatgctcg ccatcgtcga gaaggcgctg aagaagcggg cgatacccta
      241 tttctacatg gacggcgaca cggtcgaccg gcgcgacccc gtcgaccgct tcaaccgcgg
      301 cgagcgcgaa gtgttccttc tttcactcaa ggtgggcggc ttcgggctca acctcaccgg
      361 cgccgaccat gtcgtcatcg tcgatccctg gtggaaccca gcggtcgagg agcaggcgtg
      421 gtcgcgagcc catcgtatcg gccaacagcg acaggtggtg gtgtcgaaga tattctcgcg
      481 gggcaccatc gaagagaaga tactcgacct tcaacagaat aaaaaggacc tctccggctt
      541 cttcctgtcg cggagcatgg tcgagccctc gaaggacctt atccgccttc tggccgagat
      601 ggaactgcgt tcgccgggag agggcgcctg agccgccggc gcggcgcaaa aaaagaaaaa
      661 ttcgttgaaa accttgacga agcgggtcgc ccgcatataa tcacgcctcg atcatcggtt
      721 tgttcatgga aagaacaggg agttttcgat gtatgcggta gttaatttcg gcggtcatca
      781 ggtcaaggcg gtgcccggga ccaagatatt cgtcaacgcg cttcaccggg aaaagggcga
      841 ggtcttcgag aataccgagg tgctcctcgt ggccgacggt gaccggatag aggtcggcaa
      901 gccccatgtg gccggcgcga cggtcaagtt cgaggtcatc gacaccatca agggcgacaa
      961 ggtcatcgtg ttcaagaaga aacgccgcaa ggggtatcgc cggaaacgga gccaccgtgc
     1021 tcttgcgacc gcgctgaagg tggtcgagat acagcagaag aagaaataag gagacaggcg
     1081 atggcacaca aaaagagcgg cggcagcgcg cgtaacggtc gcgacagtca aggtcagcgg
     1141 cgcggcatca agatctacgg cggcgagacg gtacgcgccg gcaacatact tgtgcggcag
     1201 tgcggcacga aatggcatgc cggggagaat gtcgggaccg gtagcgacta taccctcttc
     1261 gcccttatcg acggtgtggt gaaataccag acctatcgcc gtcgcggcac cgagaagacc
     1321 cgcgtgcatg tggtgccggc cgggaccccg gaaaagtaag cgttctccgg aaacgatatt
     1381 gataagagcg gcgcgatgcc gctctttttt ttaggtgacc gtcgtgaaat tcgttgacga
     1441 agcgatcgta aaggtgcgcg ccgggaacgg cggcaagggg gcggtgagtt tcctgcgcga
     1501 gaagtatatg ccgctcggcg ggcccgacgg cggcgacggc ggcaaggggg gcgatgtcgt
     1561 catcgaggcc gacgagaatg tcggtacgct ctacgatgtc cgtttcaagc gggagataac
     1621 cgcggaaagc ggcgcggcgg ggatggggaa gaactgcttc ggccggtcgg ggaaggacac
     1681 cattgtccgc gtcccggtcg gcaccgtcat ctatgaggag gggcgggaag agccgctcgg
     1741 cgatctcgtg cggcacggcc aacgcgtcat cgtcgcgcgg ggtggtcggg gcggtcaggg
     1801 gaacgcccgg ttcaagaccg cccgccggca ggcgccgaaa ttcgcccagc cgggagaaga
     1861 agggaaggac accattgtcc gcgtcccggt cggcaccgtc atctatgagg aggggc
//
LOCUS       DAOQUE010000518         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_78136_length_1908_cov_3.895305, whole genome shotgun sequence.
ACCESSION   DAOQUE010000518 DAOQUE010000000
VERSION     DAOQUE010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78136_length_1908_cov_3.895305"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKH10_12555"
     CDS             <1..516
                     /locus_tag="PKH10_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT28994.1"
                     /translation="RVIAATNKDLEKEIVKERFRQDLYYRLSRFTLRVPPLRERREDI
                     PPLVRFFAERFAAQYKKDLRYPDEAAMARFSDRDWPGNIRELEHAVARWVLTAGDRSE
                     PGSASPTQEGAAPAAIYSFRLGEKNMKEVEKEVIEATLRFTGGNKGKTAQMLGISERT
                     IYRKMTGDEEL"
     gene            complement(520..>1908)
                     /locus_tag="PKH10_12560"
     CDS             complement(520..>1908)
                     /locus_tag="PKH10_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28995.1"
                     /translation="MEGDTLTSDDAAVSNDETIDDVLSDNDTVNCPDLKVYENVKASG
                     FPLKDKDGKITFCRPGCDTPTENDPQCVKNIWDWLNWGRYQKYKSGTESYTECYPWPC
                     VLPDTKAQSIDYSPCDRDITNKYYHADMSVLYDLKIEKDKIGLVMVGSKASKTILYDI
                     KEDTYTAIGHCGVGTAYKNERFLFFTTTEDYLTNGTYEMGDTYILSAKKTTTGYRYEV
                     IYDDEQHQAYFSRPPLVGDKWVVLNVKHRTTGATEVVYSKVDEWNWHPLTYSKVYEGN
                     VVDNRVTFINDNREIYVCDLDKLPYNPAKDCIRIDREGETPSNPRMNEQNKDQLVYYA
                     GIGAYGMTLVDISGTTPAYSLISITPSDPQTMVMGPTQFKGDLILYTEAFMPPNEETR
                     EDFKACFYRIDTKKQYCPSKPTKQEDGRYDMGFNSFDGNYQLWKTPAGTTSRFRDIAC
                     YCEKEGVCPFEE"
BASE COUNT          404 a          520 c          497 g          487 t
ORIGIN      
        1 cgggtcatcg ccgccaccaa caaggacctc gaaaaggaga tcgtgaagga gcggttccgg
       61 caggacctct actatcgcct gtcgcgtttc accctccgcg tcccgccgct ccgcgagcgg
      121 cgcgaagaca taccgccgct cgtccggttc ttcgccgaac ggttcgccgc tcagtataag
      181 aaggacctgc gctatcccga tgaggccgcc atggcgcggt tctccgaccg cgactggccc
      241 ggcaatatcc gcgaactcga acatgccgtg gcgcgctggg tgctgacggc gggcgatcgg
      301 tccgagcccg gaagcgcgtc accgacgcag gagggggcgg cccccgcggc gatctattcg
      361 ttccggctcg gtgaaaaaaa catgaaagag gtggagaaag aggtcattga ggcgacgctc
      421 cgcttcaccg gcggcaacaa ggggaaaacg gcgcagatgc tcggtatctc cgagcggacc
      481 atctaccgca agatgaccgg cgacgaggag ttgtagccgc tattcttcaa acgggcacac
      541 cccttctttc tcacagtaac aggcaatgtc acggaagcgg gaagtggttc ctgccggcgt
      601 tttccagagt tggtagttgc cgtcgaacga gttgaacccc atgtcgtaac gaccatcttc
      661 ctgcttggtt ggtttcgagg gacagtactg tttcttcgta tctatgcgat agaaacaggc
      721 cttgaagtcc tcgcgtgttt cttcgttcgg gggcatgaat gcctctgtat agaggatcag
      781 atcgcctttg aactgtgtcg gccccatgac catcgtttgg ggatcggacg gggtgatgga
      841 gatcaggctg tacgcgggcg tcgttccgga gatatccact agcgtcatgc catacgcacc
      901 tatgcccgca taatagacca actgatcctt gttttgctcg ttcatacgcg ggttggaggg
      961 cgtctcccct tcacgatcta tcctgatgca gtcttttgcc ggattgtacg gtaacttgtc
     1021 gaggtcgcag acatatattt cgcgattgtc attgatgaag gtaactcggt tgtccaccac
     1081 attcccctca tacaccttgc tgtatgtgag aggatgccag ttccattcgt ccaccttgga
     1141 gtaaaccact tccgttgcgc ctgtcgtccg gtgtttgaca ttcagaacaa cccatttatc
     1201 cccgaccaag ggcgggcgcg agaaatatgc ctgatgttgc tcgtcgtcat agatgacttc
     1261 gtaacggtag ccggtggtcg tttttttcgc cgaaagaata taggtatccc ccatttcata
     1321 agttccgttg gtgagataat cttcggtcgt cgtaaaaaac aagaaccttt cgtttttata
     1381 tgccgtgcct acgccacaat gccctattgc cgtataagta tcttctttta tatcgtacaa
     1441 tatggttttc gatgcttttg atcccaccat aaccaaaccg atcttgtcct tttctatttt
     1501 cagatcgtac aatacggaca tgtcggcgtg atagtatttg ttggtgatat cccggtcgca
     1561 agggctataa tctatacttt gcgctttcgt gtccggtaaa acacagggcc atggatagca
     1621 ttcggtatat gattcggtcc cgcttttata tttctgatat cgtccccagt taagccaatc
     1681 ccaaatattc ttcacacact gcgggtcgtt ctcggtcggc gtgtcgcacc ccggccgaca
     1741 gaaagttatc ttcccgtcct tatctttcag cggaaaccct gacgctttga cattctcata
     1801 aaccttgaga tcgggacagt tgacggtatc gttatcggaa aggacatcgt caatggtctc
     1861 atcgttagaa acagcggcat cgtcgctcgt taaagtgtcg ccctccat
//
LOCUS       DAOQUE010000519         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_78277_length_1906_cov_3.751486, whole genome shotgun sequence.
ACCESSION   DAOQUE010000519 DAOQUE010000000
VERSION     DAOQUE010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78277_length_1906_cov_3.751486"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1663
                     /locus_tag="PKH10_12565"
     CDS             <1..1663
                     /locus_tag="PKH10_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="activase"
                     /protein_id="HNT28996.1"
                     /translation="VPFDLIFDDGAEIFPNMYWFYGQQDMKAVRAVRDIPNLYLAWIS
                     NFSCAPDSFMLHYVRWMMGRKPYLVLEIDSHTADAGLDTRIEAFLDIVESYRRHALPA
                     GLPARESRYRLENRSGELVIHDARTAEQFDIRNPRVTLVWPSLGDLATEITAAASRKQ
                     GVYAEHLPVADIHSTQVARNVASGKECIPALLVLGTVLQFLGKRSPRRPDEVLLIYVP
                     STTGPCRTGQYHVFYERLFEELGFENVLILAANSDNSYREIGISFSRDMWRGVVLSDH
                     FTDARTGIRLLAQDPAEGLRLFDKVWREVVDAMARDDRALDAAIEQAARELATIPKRR
                     TLDDIRRVLIVGEIYVRRDDFSVRELFDLLIANDIFPKATGITEWVHYTDWSQRYLLG
                     REVRRRGPFTVMRPDWLRRRATLEVEGWWMHRVEEKIKRALFSTGLVPPAPDDVGAAL
                     ESGGTEFVSPELESEATVSPAVAAAGMREGYAGIAIIAPFGCLPGRLIEGVFSPWAKS
                     RGYPVIALENDGQPYPPNTIARLEVFSANVNRFPLDRVQAVSRSS"
     gene            complement(1647..>1906)
                     /locus_tag="PKH10_12570"
     CDS             complement(1647..>1906)
                     /locus_tag="PKH10_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein SpoIID"
                     /protein_id="HNT28997.1"
                     /translation="KLLIGGEKGERTVYGELVMRRLLEDLPSSLFVIDHEKAIWRFKG
                     AGWGHGVGMCQMGAVGLGQKGKKYREILSRYYPGTTVTKIY"
BASE COUNT          341 a          658 c          562 g          345 t
ORIGIN      
        1 ggtcccgttc gacctcatat tcgacgacgg cgccgagata ttccccaaca tgtactggtt
       61 ctacggtcag caggacatga aggcggtccg cgcggtgcgc gacatcccga acctctatct
      121 tgcgtggata tcgaatttct cgtgcgcccc cgactcattc atgctccact atgtccgctg
      181 gatgatggga cgcaaaccct atctggtgct ggagatagac tcgcataccg ccgacgcggg
      241 gctcgacacg cgcatcgagg ccttcctcga catcgtcgag agttaccggc gccacgcgct
      301 tccggccgga cttcccgccc gggagagccg ttaccggctt gagaaccggt caggcgagtt
      361 ggtgatacac gacgcgcgga ccgccgaaca attcgacatc cgcaacccgc gcgtgacgct
      421 cgtctggccc tcgctcggcg atctcgcgac cgagataact gccgccgcgt cgcgtaaaca
      481 gggggtctac gccgaacatt tgccggtcgc cgacatccat tcgacgcagg tggcccgtaa
      541 tgttgcatcg gggaaggagt gcatccccgc cctgctcgtg ctcggcaccg tgctccaatt
      601 cctcggaaag cgatcgccgc gccgtcccga cgaggtgctc ctcatctatg tgccaagcac
      661 caccggccct tgccgcaccg gccagtatca tgtgttctat gaacggctgt tcgaggaact
      721 cgggttcgag aatgtgctca tcctcgccgc gaacagcgac aactcctacc gcgagatcgg
      781 catcagtttc tcccgcgaca tgtggcgcgg cgtggtgctg tccgaccatt tcaccgacgc
      841 ccgcaccggc atccggctcc tcgcgcagga ccccgcggaa ggactccgtc tattcgacaa
      901 ggtgtggcgc gaagtggtcg atgcgatggc gcgggacgac cgggcgctcg acgcggcgat
      961 cgaacaggcg gcgcgggaac tggccacgat accgaaacga cggacgctcg acgacatccg
     1021 gcgggtgctc atcgtcggcg agatctatgt gcgacgcgac gatttctcgg tgagggagct
     1081 gttcgaccta ctcatcgcca atgacatatt ccccaaggcg accggcatca ccgaatgggt
     1141 ccactacacc gactggtcgc aacgctacct gctcggtcgg gaggtgcgac ggcgcgggcc
     1201 gttcaccgtc atgcgccccg actggctccg gcggcgggcg acgctcgagg tcgaaggatg
     1261 gtggatgcac cgcgtcgaag agaagataaa gagagctctc ttttccaccg gactcgtgcc
     1321 gccggccccc gatgatgtcg gcgccgcgct cgaatccggc ggcacggaat tcgtgtcgcc
     1381 cgaactggag agcgaagcga ccgtatcgcc cgccgtcgcc gccgccggca tgcgggaagg
     1441 ttacgcgggc atcgccatca tcgccccctt cggctgtctt cccggccggc tcatcgaggg
     1501 agtgttcagc ccgtgggcca agtcacgcgg gtatccggtc atagcgctcg agaacgacgg
     1561 ccaaccctac ccacccaata ccatcgcgcg acttgaggtg ttctcagcga atgtgaaccg
     1621 gttcccgctc gaccgggtcc aggccgtcag tagatcttcg tgaccgtcgt tccgggatag
     1681 tatctcgaca gtatctcgcg gtacttcttc cctttctgcc ccagccccac cgcacccatc
     1741 tggcacatac cgaccccatg cccccacccg gcacccttga agcgccagat cgccttctcg
     1801 tgatcgatga cgaacagcga actcggcagg tcctcgagca ggcggcgcat caccagttcc
     1861 ccgtacaccg tccgttcccc cttctcgccg ccgatgagca gtttcc
//
LOCUS       DAOQUE010000520         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_78481_length_1903_cov_4.093615, whole genome shotgun sequence.
ACCESSION   DAOQUE010000520 DAOQUE010000000
VERSION     DAOQUE010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78481_length_1903_cov_4.093615"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PKH10_12575"
     CDS             complement(<1..1181)
                     /locus_tag="PKH10_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HNT28998.1"
                     /translation="MDIEKKMQILGAAAKYDVSCSSSGSRRPTPQGGSGNGVSAGICH
                     SWADDGRCISLLKTLFTNVCIHDCAYCVNRRSNDIPRAAFTVDEICELTMEFYRRNYI
                     EGLFLSSGVMRNADYTMERLTLVAKKLRDEHRFGGYIHLKAIPGASRELIRQAGLHAD
                     RISVNIELPTEKSLLLLTDKKKDDIMLPMGEISTAIVENRDERKHLRSAPRFAPAGQS
                     TQLIVGATPENDHQIVRLSEGLYNHYGLKRVYYSAFVPVSSDNRLPALATPPLLREHR
                     LYQADWLMRYYGFAAGEILDDRTPFLDEEMDPKSAWALRNMHLFPIDIQTAPYESLLR
                     VPGIGVRSAQRIVIARRHSRLGYGDLKKIGVVMKRAKHFLTVGGRMLLPGSGRPLRLI
                     RR"
     gene            1339..>1903
                     /locus_tag="PKH10_12580"
     CDS             1339..>1903
                     /locus_tag="PKH10_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT28999.1"
                     /translation="MGRGILVFLGIFLMGALLMATEVRKPKIAVMEIEDTAQRFDAAL
                     IENATEILRSKLTATGRFVVIDKSRQHEKLKSLIKEEKRESYKECYDQKCQIPLGQAL
                     AADTILRSSISCINQNCVLSSELVDLAKEAAIRGGSAEFRYDPDNPEALMPAIVEVVR
                     QLAPAPAAPLAPIPTPKEPVIKRTHPYL"
BASE COUNT          373 a          543 c          595 g          392 t
ORIGIN      
        1 cggcggatga ggcgaagcgg gcgaccggac cccggtaaca acatccggcc gcccacggtg
       61 aggaaatgtt tcgcccgctt catgacgacg ccgatcttct tgaggtcgcc gtagccgagg
      121 cggctatgtc gccgcgcgat gactatccgc tgggcgctcc ggacaccaat gccggggaca
      181 cgcagaagcg attcgtacgg cgcggtctgg atatctatgg ggaacaggtg catattgcgg
      241 agcgcccacg cgcttttcgg atccatctcc tcgtcgagga acggcgtccg gtcgtcaagt
      301 atctctcccg ccgcgaaacc gtaatagcgc atgagccagt cggcctgata gagacggtgt
      361 tcacgcagga gcggcggggt ggcaagcgcc gggagacggt tgtcgctcga caccgggaca
      421 aaggccgagt agtagacccg cttgaggccg tagtggttgt acagcccctc cgacagacgg
      481 acgatctgat ggtcgttctc gggggtcgcg ccgacgatga gttgggtgga ctgtcccgcc
      541 ggggcgaaac gcggcgccga gcggagatgt ttccgttcgt cgcggttctc cacgatggcg
      601 gtcgatatct cgcccatcgg aagcatgatg tcatcctttt tcttatcggt aaggaggagg
      661 agcgactttt cggtcggcag ttcgatgttg accgatatac ggtcggcgtg gagccccgcc
      721 tgccgtatca gttcgcggct cgcccccggt atcgccttca agtggatgta gccgccgaag
      781 cggtgttcat cgcgcagttt cttcgcgacg agcgtcaacc gctccatcgt gtagtcggcg
      841 ttccgcatca cgccggaact caggaacagc ccttcgatgt agttgcggcg gtagaactcc
      901 atcgtgagtt cgcagatctc gtcgacggtg aaggcggcgc gggggatgtc gttggaccgg
      961 cggttcacgc agtaggcgca gtcgtggatg cagacattgg tgaaaagcgt tttgagaagg
     1021 ctgatgcagc ggccgtcgtc ggcccatgag tggcagatgc cggctgagac gccgttgccg
     1081 cttcccccct gcggcgtggg gcgacggctt ccgctcgacg agcacgacac atcgtatttg
     1141 gccgccgcgc cgagtatctg cattttcttc tcgatatcca ccgacggtcc tccgtgacaa
     1201 cagaactacc gtacggtatc ttgtaaggca tgtcaagaaa tttgtcaggt atttgttcgg
     1261 caacgcgcca tcgcttttct tgactcactt taatattctt tgttattgtt tgacacgaat
     1321 gctggtatgg agcaaaagat gggacggggc atacttgttt ttctcggcat cttccttatg
     1381 ggtgcgttgc tgatggcgac cgaggtacgc aagccaaaga tcgccgtcat ggagatagag
     1441 gacaccgcgc aacggttcga cgcggcgctc atcgagaacg ccaccgagat actgcggtcg
     1501 aaactcacgg cgaccggtcg tttcgtcgtc atcgacaagt cgcgccaaca cgaaaaactg
     1561 aaaagcctga tcaaagagga aaaacgcgag tcatataaag aatgttacga tcagaaatgc
     1621 cagataccgc tcgggcaggc gcttgccgcc gatacgatac tgcgatcgtc catctcctgc
     1681 atcaatcaaa attgtgtcct ctcgtcggaa ctggtcgatc tcgcgaaaga ggcggccatt
     1741 cgcgggggga gcgccgagtt ccgctacgat cccgacaacc ccgaggcgct gatgccggcc
     1801 atcgtcgagg tggtgcggca gttggcgccc gctccggcgg cgcctttagc gccgatcccg
     1861 acgccgaagg agcctgtcat caagcggacc catccttatc tgt
//
LOCUS       DAOQUE010000521         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_78801_length_1898_cov_4.937602, whole genome shotgun sequence.
ACCESSION   DAOQUE010000521 DAOQUE010000000
VERSION     DAOQUE010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78801_length_1898_cov_4.937602"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKH10_12585"
     CDS             complement(<1..299)
                     /locus_tag="PKH10_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29000.1"
                     /translation="MRYMILKSETTGTVCGWRTAWGVLLLLFAACTSHATKNDTDTVP
                     IDSDWVVTDDAGVLEEESGILDEDIVNDETLEDGDFKSDADTHIGPGDKEPVEVD"
     gene            complement(354..764)
                     /locus_tag="PKH10_12590"
     CDS             complement(354..764)
                     /locus_tag="PKH10_12590"
                     /inference="COORDINATES: protein motif:HMM:NF025157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HNT29001.1"
                     /translation="MTFDPTKPFNDLPLLPPAVDIETRPILKQLAHSRSALAELKGYA
                     DMIPNKRLLLDAVVLLEAKDSSAIENIVTTHDELYKALVADPATLVAQGLCERRTASK
                     YLSELEAAGLLISQKDWKTKIFINTPLFDLLKRS"
     gene            898..984
                     /locus_tag="PKH10_12595"
     tRNA            898..984
                     /locus_tag="PKH10_12595"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Leu,seq:cag)
     gene            complement(999..1607)
                     /locus_tag="PKH10_12600"
     CDS             complement(999..1607)
                     /locus_tag="PKH10_12600"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29002.1"
                     /translation="MLQPIRPGVALPTETIADRPLDLPALFPGLVRFECEIGFCSAIF
                     LNEYANRHPDTGILGIEKKRSYFLRGLHNLERKLKQDNVRIINFDAHPVFLMLVPPAT
                     LDALHIYFPDPWPKKRHLRNRLVNRDNLRMFHDRLRPGGRIYLATDHPDYAAFIEREL
                     TAVAELFTRRPYGTADREVLTKWERKQIENGTHVNYYLLEKR"
     gene            complement(1609..>1898)
                     /locus_tag="PKH10_12605"
     CDS             complement(1609..>1898)
                     /locus_tag="PKH10_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNT29003.1"
                     /translation="VPAEELARYAGQEVLIVEDIVDTGLTLEVFSAALKGAGARSVRI
                     CSFLEKSEVNRGRVKIDFLGFPIPDKFVVGYGLDCDEKHRDLPHVAIFDED"
BASE COUNT          344 a          502 c          605 g          447 t
ORIGIN      
        1 tcatccacct cgaccggctc cttgtcaccg ggaccgatgt gggtgtcggc atcggatttg
       61 aaatctccgt cttccagtgt ctcgtcattg actatatctt catcgaggat gccagattct
      121 tcttccagca cccctgcatc atccgttacc acccaatcac tatcgatcgg taccgtgtcg
      181 gtgtcgttct tggtggcgtg tgaggtgcag gcggcgaaga gaaggagcaa aaccccccac
      241 gcggttcgcc agccgcagac cgtacccgtc gtttcgcttt ttaagatcat gtacctcatt
      301 tgctcatgag cacgctgtcg tgtgcataaa aagcaatttt atgctcatga tgctcaactt
      361 ctttttaaca ggtcgaagag cggcgtattg ataaagatct tggttttcca gtctttttgt
      421 gagatcagaa gcccggccgc ttccagttca gagagatact tgctcgccgt gcgccgttcg
      481 cacagacctt gagcgaccag cgtggcgggg tcggcgacca gcgccttata cagttcgtcg
      541 tgcgtggtga cgatgttctc tatggcggaa ctgtccttgg cctccagcag gacgaccgcg
      601 tcgagcagga gccgcttgtt cggtatcatg tcggcgtagc ctttcagttc ggcgagggcg
      661 ctgcgggaat gggccagttg tttgagtatc ggccgcgttt cgatgtcgac ggccgggggg
      721 agtagcggta gatcgttaaa gggtttggtc gggtcaaagg tcatgggcgt tctctccctg
      781 caatacggga gggattatag cgagccgcga aatgaaagta aagcctccgt cggcccgata
      841 tttcttgaca atggccgccg ccgtgtataa tcgggaacgg ttttttgaca accgggtgcc
      901 gggatggtga aattggcaga cgcgctagat tcaggttcta gtgggggcaa ccctgtgcag
      961 gttcgattcc tgttcccggc accatttttt ctttccattc agcgtttttc cagcaggtag
     1021 taattgacgt gggtcccgtt ctctatctgc tttctctccc atttggtcaa cacttcgcgg
     1081 tcggcggtgc cgtagggccg tcgcgtgaac agttcggcga ccgccgtcag ttcccgttcg
     1141 atgaaggcgg cgtagtcggg atggtcggtc gcgaggtata tccgtccgcc gggccgcaga
     1201 cggtcgtgga acatccgaag gttgtcgcgg ttcaccaacc ggttgcgcag gtgccgcttc
     1261 ttgggccacg gatcggggaa gtagatgtga agcgcgtcca gcgtggcggg gggaacgagc
     1321 atcaggaaca ccgggtgggc gtcgaaattg atgatgcgga cattgtcctg tttcagtttc
     1381 cgttcgagat tgtgcaggcc gcgcagaaaa taggagcgtt tcttttcgat gccgagtatg
     1441 ccggtgtcgg gatgccggtt ggcgtattcg ttcaggaaga tggcggagca gaaaccgatc
     1501 tcacactcga agcggacaag acccgggaag agcgccggga gatcgagcgg gcggtccgct
     1561 atggtctcgg tcggcagggc gacgccgggg cgtatcggct ggagcatgtc agtcttcgtc
     1621 gaagatggcg acatggggca ggtcgcggtg cttctcgtcg cagtcgagcc cgtagccgac
     1681 gacgaacttg tcgggtatcg ggaagccgag gaaatctatc ttcacgcggc ccctgttcac
     1741 ctcgcttttc tcgaggaacg agcagatgcg gaccgaccgg gcgccggcgc ctttgagcgc
     1801 cgccgagaag acctcgaggg tgagtccggt gtctacgatg tcctcgacga tgagcacttc
     1861 ttgtccggcg tagcgcgcga gttcctcggc ggggaccg
//
LOCUS       DAOQUE010000522         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_78932_length_1896_cov_3.994025, whole genome shotgun sequence.
ACCESSION   DAOQUE010000522 DAOQUE010000000
VERSION     DAOQUE010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78932_length_1896_cov_3.994025"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PKH10_12610"
     CDS             complement(<1..831)
                     /locus_tag="PKH10_12610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT29004.1"
                     /translation="MRILFINKSDAGGGAATAMNRIADGLAKLGAENYFIVAKRKSGL
                     PNVYATRGRYGWLIEEAIDRLTRRFGAEGLCFPFSGPAIRRMAHALKPDVIVLNNIHS
                     GYFPLSIMPELARIAPLYWLVHDLWPVTGHCMAPIDCANWQDACGNCPRLDTYPSLGS
                     DRTKKLAERKRRIYDEVKPTFIVASRWMDDILRKSPLTAHHRIVRIPHGIPADIFSPQ
                     DKNSARKRFGITDHAPVLLFAAEHLDNRLKGGDLLRDILQRLDEQLVAPAHLVASGKG
                     K"
     gene            complement(906..>1896)
                     /locus_tag="PKH10_12615"
     CDS             complement(906..>1896)
                     /locus_tag="PKH10_12615"
                     /inference="COORDINATES: protein motif:HMM:NF018880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29005.1"
                     /translation="RRGPKARPPCADADVPAGDDTVDDDSSIETDETTTPDEDAATVD
                     VYAPGPHVVLQKDVAENADGNAQAVRIFYPAEAGTYPYINFVHGFQLKNIYYDQILTQ
                     LASHGFIIVSPQLKQSLIGGDNSIVEADKILALIADWVKPKLATHTGIAVPDFTRFGL
                     TGHSRGSKISWRMILKTPAEFHAAMGIDPVDTNSTATSDPHAITGPMTYDAPSAAVGT
                     EKGPTGFQACAPADSNSAGIFYPNFPSPAWHLIAGGVGHMDMIDADDLGSCGMTCSVC
                     SGGDDAQKATFRTLVGGLLVAFFRAALYGETALYDELVDTNGMPHPITTAEHK"
BASE COUNT          337 a          511 c          639 g          409 t
ORIGIN      
        1 tttgcccttg cccgatgcga cgagatgggc cggggcgacg agttgttcgt cgagccgttg
       61 caggatatct cgcagaaggt cgcccccctt gagccggtta tcgagatgct cggcggcgaa
      121 cagaagtacc ggagcatggt cggtgatgcc gaaccgtttc cgtgccgagt ttttgtcctg
      181 tgggctgaag atgtccgccg gtatgccgtg cggtatccgg acgatgcggt gatgcgccgt
      241 gagcgggctt ttgcgcagga tgtcatccat ccagcgcgat gcgacgatga atgtcggttt
      301 cacctcgtca tagatgcgcc gtttccgttc ggcgagtttc ttggttcgat cgcttccgag
      361 cgacgggtag gtatcgaggc gggggcagtt cccgcaggca tcctgccaat tcgcgcagtc
      421 gataggcgcc atgcagtgcc ccgtgacggg ccacagatcg tggacgagcc agtagagcgg
      481 cgcgatacgg gccagttccg gcatgatcga aagggggaaa tagccgctgt ggatgttgtt
      541 gagcacgatg acatcgggtt tcaatgcgtg cgccatgcgc cgtatcgccg gaccggagaa
      601 ggggaaacag agcccttcgg cgccgaaccg gcgcgtcagg cggtcgatcg cctcctcgat
      661 gagccagccg tagcgaccgc gcgtagcata cacattcggt agaccgcttt tgcgtttggc
      721 gacaatgaaa tagttctcgg cgccgagttt cgcgagtcca tcggcgatgc gattcatcgc
      781 ggtcgccgcg ccgccgcccg cgtcgctctt gttgatgaac aggatacgca tcgaaactcc
      841 attactgacg gtgaagtatc gtggtgtacg agtatgaagt caaattttag gggaagcgat
      901 gatggctact tgtgttccgc agtcgttatc gggtggggca tgccgttggt gtcgacgagt
      961 tcatcgtaca gcgccgtttc tccgtagagc gcggcgcgga agaaggcgac cagaaggccg
     1021 ccgacgagcg tgcggaaggt cgctttttga gcatcatctc cgccgctgca tactgaacag
     1081 gtcatgccgc aggagccgag gtcgtcggca tctatcatgt ccatgtggcc cacgccgccc
     1141 gcgataaggt gccaagcggg ggaggggaag ttgggataga atatgccggc gctgttggaa
     1201 tcggccggag cgcaggcctg aaagcccgtc ggcccctttt cggtaccgac ggcggctgac
     1261 ggcgcatcgt aggtcatggg gccggttatg gcgtgaggat cgctcgtggc ggtggagttg
     1321 gtatcgaccg ggtctattcc catggcggca tggaattcgg caggtgtttt cagtatcatg
     1381 cgccacgata tcttcgaccc gcgcgaatgg ccggtcagcc cgaatctcgt gaaatcgggc
     1441 accgcgattc cggtgtgcgt agcgagtttc ggttttaccc agtcggcgat gagagcgagg
     1501 atcttgtcgg cctcgacgat gctgttgtcg ccgccgatga gactctgttt taactgggga
     1561 gatacgatga tgaacccatg gcttgcgagt tgcgtgagta tctggtcgta ataaatgttc
     1621 ttgagttgga agccgtgcac gaagttgatg tagggataag tgcccgcctc ggccggatag
     1681 aagatgcgga cggcctgcgc gttgccgtcg gcgttctcag cgacatcctt ctgaagcacg
     1741 acatgaggcc ccggcgcata cacatcgacc gtcgcggcat cctcgtcggg ggttgtcgtt
     1801 tcgtcggtct caatactgct atcatcgtcg accgtatcgt cgcctgccgg cacatcggcg
     1861 tcggcgcatg ggggacgggc ctttgggccc cgccgg
//
LOCUS       DAOQUE010000523         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_79522_length_1887_cov_4.546943, whole genome shotgun sequence.
ACCESSION   DAOQUE010000523 DAOQUE010000000
VERSION     DAOQUE010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79522_length_1887_cov_4.546943"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="PKH10_12620"
     CDS             complement(<1..1288)
                     /locus_tag="PKH10_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29006.1"
                     /translation="MFAKFRYLLPMLLVSSFLCAAPAIAVLPIGANSDAKEDAAGEVF
                     KKAKPALSAIKGLALDALSKQEKRAVLDCEGEVSCMHRAVRQNGSDYYIIPVVARYNE
                     DDDEVTIELYFYNGNESVKDSERIGPATPKKAAKLFSSVIENFVAKAIKRESRLKEKE
                     ESTAKKSKKYRSGSEEIEALVADAEREQEEETPKDHAAKGYRAYREGDLKAAEQYFER
                     AGKKNVKAKKIAEAIRDIAKKKRDADAFVEQKRFDDAIRVIEEIEKKDLGIRTLGHQE
                     GVFSSEKRSRVLYEDPSRSDMEKVEKIHQKFVKKIAEARQAKLQKVAEVEKALNEKIL
                     VREKRIAQFVDEDKQQDLKEKETEAALKKRIGDLRYKWEKEDGELEQQIVALESKLTM
                     LEQKEKGVIKVSTEKRDKEKADEFAAVDKKYKEDRKK"
     gene            1508..>1887
                     /locus_tag="PKH10_12625"
     CDS             1508..>1887
                     /locus_tag="PKH10_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HNT29007.1"
                     /translation="MAKKKKIIIMGAAGRDFHNFNTVYRNCESCEIVAFTAAQIPDIN
                     DRKYPAELAGPLYPNGIPIYDEADLPKLIKEHKVDEVVFAYSDVTYKYVMNRSALVNA
                     LGADFKLIGYEKTKVRSVKPVISI"
BASE COUNT          375 a          577 c          434 g          501 t
ORIGIN      
        1 gtttcttacg gtcttccttg tacttcttgt cgaccgccgc gaattcgtcc gccttctcct
       61 tgtcgcgctt ttcggtcgaa acctttatga cgcccttctc cttctgctcg agcatggtca
      121 gtttgctttc cagcgcaacg atctgctgct cgagttcacc gtcctccttt tcccatttgt
      181 atcggagatc gccgatgcgc ttcttcaatg ccgcctcggt ctctttttcc ttcagatcct
      241 gctgcttgtc ctcatcgacg aactgcgcga tcctcttctc acggacgagt atcttttcgt
      301 tcagcgcctt ctcgacctcg gcgaccttct gcagtttcgc ctgccgcgcc tcggcgatct
      361 ttttgacgaa cttttggtgg atcttctcga ccttttccat gtcactgcga gaagggtcct
      421 catacaggac gcgggaacgc ttctcactgc tgaacacacc ttcctgatgt cccagcgtac
      481 ggatgccgag atcctttttc tctatctctt cgatgacgcg gatggcgtca tcgaaacgct
      541 tctgctccac gaacgcatcg gcatcgcgtt tctttttcgc gatatcgcgg atcgcttcgg
      601 cgatcttctt cgccttcaca ttcttcttgc cggcgcgttc gaaatactgt tcggccgcct
      661 tgaggtcacc ttcccgatag gcccgatatc ctttcgcggc atggtccttg ggcgtctcct
      721 cttcctgctc ccgctccgca tcggcgacca gcgcctctat ctcctcgctc ccgctccggt
      781 actttttgct tttcttcgcc gtcgattctt ctttctcctt cagccgcgat tcccgcttta
      841 tcgctttcgc aacaaaattc tcgatcacgg aggaaaaaag cttcgccgct tttttaggcg
      901 tcgcaggacc gatgcgctcc gaatccttga ccgactcgtt accgttgtag aaataaagtt
      961 ctatggtgac ctcgtcatcg tcctcattgt aacgggcgac aaccgggatg atgtaataat
     1021 cgctaccatt ctgcctgacg gcacgatgca tgcacgagac ctctccttca caatcgagaa
     1081 cggcacgctt ttcctgtttg gaaagtgcat cgagcgcaag ccccttgatg gcggaaagcg
     1141 cgggtttcgc ttttttgaag acctcgccgg ccgcatcctc tttcgcgtcg ctgttggcac
     1201 cgatgggaag tacggctatc gcgggagcgg cgcacaggaa agacgagacc agaagcatcg
     1261 gcaacaggta tctgaacttc gcgaacatct acttctccct cttgtcatcg ttcaaacggg
     1321 ccaaccgggt tcatactatc aactataaga aaacgaagtc aattttttaa cgcgataaac
     1381 acaaaaaaaa tcacgcgata tcgcgatttt cgtttccttg actttgacct ttttcctgtt
     1441 atcgtcgatt cgccgtagac agtgtccggc cgtgatttaa caacacttca gcctgaagga
     1501 gaagatcatg gcgaagaaaa agaagatcat catcatgggc gccgccggaa gagatttcca
     1561 caacttcaac accgtgtatc gcaattgcga atcctgcgag atcgtcgcgt tcaccgcggc
     1621 gcagatcccc gacatcaacg atcgcaagta tcccgcggaa cttgccggtc cgctctatcc
     1681 gaacgggatc ccgatatacg acgaggccga tcttccgaaa ctcatcaagg agcacaaggt
     1741 cgacgaggtc gtcttcgcct acagcgatgt cacctacaag tatgtgatga accgcagcgc
     1801 gctcgtcaac gcgctcggcg ccgacttcaa actcatcggc tacgagaaga ccaaagtgag
     1861 atcggtgaag ccggtcatca gcatctg
//
LOCUS       DAOQUE010000524         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_79874_length_1882_cov_3.669951, whole genome shotgun sequence.
ACCESSION   DAOQUE010000524 DAOQUE010000000
VERSION     DAOQUE010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79874_length_1882_cov_3.669951"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1724
                     /gene="secD"
                     /locus_tag="PKH10_12630"
     CDS             <1..1724
                     /gene="secD"
                     /locus_tag="PKH10_12630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNT29008.1"
                     /translation="RMEKNWTMRGFFILALIILSVVLLLPSFMDREQYPWLGKINDGL
                     NLGLDLKGGIHFVLSVDVDKAVQDRVDRQTDEMTKRMEEEQVAFASIKADPVNPVIRI
                     TLQNESDADKFRDKVIDYFRTFRKTGQDGLTYELTMREDYIGNLKETSVDQAIETLRS
                     RIDALGLKEPVIVKQGANSILIQLPGYKDVERARSIIGQTAQLEFKIVAEDRDPLNGL
                     TDIPDGITLQQGRGQNSDGGMLAYQYAVAPDKDALLGWLKDKAPEGTEFLVEEIIRGD
                     GKKEYMSWLVEKKAYLTGDMLTDARVEVDQQRNRPYVSLSFDRNGARIFEEVTGKFVK
                     RKMAIVLDNRVNSAPQIQTKISGGNAMITLNSMNDFNTTLTEAKDLALVLRAGSLPAP
                     VTFEENRTVGPSLGADSIEKGKISVAVGMLVVVLAMVIYYGFSGLVADLALLLNILFI
                     MSFMATFGATLTFPGIAGIVLTIGMAVDANVLINERIREELRNGHSFLTAFELGYERA
                     FSAIFDSNLTTAIAGFFLWNFGSGTVKGFAITLLIGIASSLFTAVYVTRVVFHLAMKL
                     GFKKISV"
BASE COUNT          413 a          557 c          518 g          394 t
ORIGIN      
        1 gccgtatgga aaagaactgg acgatgagag gatttttcat cctcgcgctc atcatcttgt
       61 ctgtcgtttt gcttctcccc tccttcatgg acagggaaca gtacccatgg ctcgggaaga
      121 taaatgacgg gctgaacctc ggtcttgacc tcaagggcgg gatccatttc gtcctttcgg
      181 tcgatgtgga taaggcggtg caggatcgcg tggaccgcca gaccgacgag atgaccaaac
      241 ggatggagga ggagcaggtc gccttcgctt ccatcaaggc cgacccggtg aatcccgtca
      301 tacgcatcac attgcagaac gaaagcgacg cggacaaatt ccgcgacaag gtgatcgatt
      361 atttccgcac cttccgcaag accgggcagg acgggctcac ctatgagttg acgatgcggg
      421 aggactatat cggcaacttg aaggagacct cggtcgatca ggcaatagaa acgcttcgtt
      481 cccgcatcga cgccctcggt ctcaaggagc cggtcatcgt caaacagggc gcgaactcca
      541 tacttatcca gttgcccggc tacaaggatg tcgaacgggc ccgatccatc atcggccaga
      601 cggcccagtt ggagttcaag atcgtcgccg aggaccgtga tccgctcaac ggcctcaccg
      661 acataccgga cggtatcacc ctgcagcagg ggcgcgggca gaactccgac ggcggcatgc
      721 tcgcctacca atatgccgtt gcgcccgata aggacgcgct cctcggctgg ttgaaggaca
      781 aggcgcccga aggcaccgag ttcctcgtcg aggagatcat tcgcggggac ggcaaaaaag
      841 aatatatgtc gtggctggtc gagaaaaaag catatctcac cggtgatatg ctgaccgacg
      901 cacgcgtcga ggtggaccaa cagcgtaacc gcccctatgt cagcctgtca ttcgaccgca
      961 acggggcccg gatattcgag gaggtcacgg ggaaattcgt gaaacggaaa atggccatcg
     1021 tcctcgacaa ccgcgtcaat tccgcccctc agatacagac caagatatcg ggcggcaacg
     1081 cgatgatcac tctcaattcg atgaacgatt tcaataccac gctgaccgag gccaaggatc
     1141 tcgcgctggt gttgcgcgcc ggttcgctac ccgctccggt caccttcgaa gagaaccgga
     1201 cggtcgggcc cagcctcggc gccgattcga tcgagaaggg caagatatcg gtcgctgtcg
     1261 gcatgctcgt ggtcgtgctg gctatggtca tctactacgg tttttcggga cttgtcgccg
     1321 acctggcgct cctgctcaac atactattca tcatgtcctt tatggccacc ttcggagcga
     1381 cattgacatt ccccggcatc gccggcatcg tgctcacgat cggcatggcg gtcgacgcga
     1441 atgtgctcat taacgagcgt atccgcgaag aattgcgtaa cgggcactcg ttcctgaccg
     1501 cctttgaact cggttacgag cgcgccttct ccgccatatt cgattcgaac ctcacgaccg
     1561 cgatcgccgg cttcttcctg tggaatttcg ggagcggcac cgtcaaggga ttcgctataa
     1621 ccctcctcat cggcatcgca tcgtccctgt tcaccgccgt ctatgtgacc cgcgtcgtgt
     1681 tccatcttgc gatgaaactc ggcttcaaga agatatcggt ttaggagggc tctgtcatgg
     1741 aactgatcaa gaacacactc aacatagatt tcgtcgggaa aataaaaatc ttcgccatcc
     1801 tttcgtccat cgcggttctc gccggcatcg tccttctgct cgttatcggc cccaactacg
     1861 gtgtcgattt caagggaggc ac
//
LOCUS       DAOQUE010000525         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_80689_length_1870_cov_3.740496, whole genome shotgun sequence.
ACCESSION   DAOQUE010000525 DAOQUE010000000
VERSION     DAOQUE010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80689_length_1870_cov_3.740496"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /gene="hisIE"
                     /locus_tag="PKH10_12635"
     CDS             <1..746
                     /gene="hisIE"
                     /locus_tag="PKH10_12635"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNT29009.1"
                     /translation="MLQGPAFDRIEAVRALTKKPITAAGGVTTVAEVARLDRMGVDAQ
                     VGMALYTGKMTPAACFLETLDWEKAPLIPAITQDAAGQVLMLAYMNTEALEKTLTTGR
                     VTYWSRSRKELWTKGDTSGHYQTLRAIRRDCDGDALLLTVAQEGPACHTDRYSCFGDR
                     RFSLNELYGVLAARIASGDAKSYTAKLSPKELRQKLLEEAAEVALADDRDNKVWEAAD
                     LLYFLTVLLAKEKIGLDEVMNELQRRRRK"
     gene            743..>1870
                     /gene="hisD"
                     /locus_tag="PKH10_12640"
     CDS             743..>1870
                     /gene="hisD"
                     /locus_tag="PKH10_12640"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNT29010.1"
                     /translation="MMRIIKAADIPASFYEYREIAESDTVRAIIDDVKRRGDEAVRFY
                     TETFDLVKLSDLRVPESELEKGLAEITPELKKALELAAGNIRRFAEAQRKGLTDMSIE
                     TMPGVIAGQRVIPVERAGIYAPGGRFPLPSSVLMGVIPALAAGVKEVALFTPPRKDGT
                     ILPAIRAAALIAGCREVYRVGGVQAVAAMAYGTATIRPVQVIAGPGNRFVAAAKKQVY
                     GAVGIDFIAGPSEVLIIADDSADPRVVAADLLAQAEHDPDASAILLTTSGKLAQAVAA
                     EVERQLALLPTAGTARPSIEDNGLIVLCDSLDDAVTVANKKAPEHLELDIRDAEKLVP
                     RLTNYGALFIGARSAEALGDYAAGPNHTLPTGGAARYTGGLS"
BASE COUNT          350 a          586 c          652 g          282 t
ORIGIN      
        1 ggatgctgca gggtcccgcc ttcgaccgca tcgaagcggt gcgggcgctg acaaaaaagc
       61 ctatcaccgc ggcgggcggc gtcacgacgg tcgccgaggt ggcgcggctc gaccggatgg
      121 gggtcgacgc gcaggtcggg atggcgctct acaccgggaa gatgaccccg gccgcatgtt
      181 tccttgaaac gctcgactgg gaaaaagcgc cgcttatccc ggcgataacg caggatgcgg
      241 cggggcaggt attgatgctc gcctacatga atacggaggc gctcgaaaag acgctcacga
      301 ccggcagggt cacctattgg tcgcggtcgc gcaaggagct ttggaccaaa ggcgatacct
      361 cgggtcacta ccagacgctc cgcgccatcc ggcgcgactg cgacggcgac gcgctgcttc
      421 tgaccgtggc gcaggaggga cccgcctgtc acaccgaccg ctactcctgt ttcggcgacc
      481 gccgtttctc gctgaacgaa ctctacggcg tccttgcggc gcgcatcgca tcgggcgacg
      541 cgaagagtta cacggcgaaa ctgtcgccca aggaactgcg gcagaaactg ctcgaggagg
      601 cggccgaagt ggcgcttgcc gacgatcgtg acaacaaggt gtgggaggcg gccgatctgc
      661 tctatttcct gacggtgctg ttggccaagg agaagatagg gctcgacgag gtgatgaacg
      721 aactgcagag acggagacgg aaatgatgag gatcataaaa gcggccgata tcccggcgtc
      781 gttctatgaa taccgcgaga tagccgagtc cgacacggtg cgggcgatca tcgacgatgt
      841 gaagcggcgc ggcgacgagg cggtccgctt ctacaccgag accttcgacc tcgtgaaact
      901 gagcgacctg cgggtccccg aaagtgaact cgaaaagggg ctcgccgaga tcacgccgga
      961 acttaagaag gcgctcgaac tggcggcggg gaacatccgc cgctttgccg aggcgcagcg
     1021 gaaagggctc accgatatga gcatcgaaac gatgccgggg gtgatcgcgg ggcaacgggt
     1081 gatcccggtc gaacgggccg gcatctatgc gccgggcggc cgcttcccgc tcccgagttc
     1141 ggtcctcatg ggggtcatcc cagcgctcgc ggccggggtg aaagaggtgg cactcttcac
     1201 gccgccgcgc aaggacggca cgatactccc ggcgatacgg gccgcggcgc tcatcgccgg
     1261 ctgtcgcgag gtctatcgcg tcggcggggt gcaggcggtc gctgcgatgg cctatggcac
     1321 cgcgaccatc cggccggtgc aggtgatcgc cgggccgggg aaccggttcg tcgccgccgc
     1381 gaaaaaacag gtctacggcg cggtcggcat cgatttcatc gcgggaccct ccgaggtgct
     1441 catcatcgcc gacgattcgg ccgatccccg cgtggtggcg gccgatctgc tcgcgcaggc
     1501 ggagcacgac cccgacgcct cggcgatact gctgaccact tccgggaaac tcgcgcaggc
     1561 ggtggcggcc gaagtggagc ggcaactggc gctgctcccg accgccggga cggcgcggcc
     1621 gagcatcgag gacaacgggc tcatcgtcct gtgcgactcg cttgatgatg cagtgacggt
     1681 ggcgaataaa aaggcgcccg aacaccttga actcgatatc cgcgacgccg agaagctcgt
     1741 cccgcgcctc accaattacg gcgcactctt catcggggcg cggtcggccg aggcgctggg
     1801 cgactatgcg gcgggtccga accacacgct cccgaccggc ggtgcggcgc gctacaccgg
     1861 cgggctgtcg
//
LOCUS       DAOQUE010000526         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_81614_length_1857_cov_3.231410, whole genome shotgun sequence.
ACCESSION   DAOQUE010000526 DAOQUE010000000
VERSION     DAOQUE010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81614_length_1857_cov_3.231410"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PKH10_12645"
     CDS             complement(<1..418)
                     /locus_tag="PKH10_12645"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNT29011.1"
                     /translation="MLWMIADTTRTPSVVATVESVARMATAGIHKVTIRNDGVFSLAE
                     LLKAAERIVARCPGVEVFFAGSPEQARRCGVRGLHLRSSMLDHVAAIRATAPDLAIAV
                     STHTTGEFERAFGSGADLAFYAPVFPPYSKPNDTRPT"
     gene            complement(497..1603)
                     /locus_tag="PKH10_12650"
     CDS             complement(497..1603)
                     /locus_tag="PKH10_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29012.1"
                     /translation="MRGLMFLMTLFLAVILRADPPEQTWIECDALNDCPEQMTCVKFD
                     DMPTPVCWPADQDPCEYCGTAECAVAESYPPQVTCGDGEVPDTDPVTCVSDEDCPEKM
                     FCFSGYCVDEGDPATEKTCENEAGYCVIGETSGYCQCVTGAGVGWGEAEPGEPTDPVE
                     DGDGDGEVPPPPFFLPTSDECMAKLVENCGETAPDLKEECGAELFDACLDAVEWVEGT
                     CFETTLTEEQRNAIAKGEWVGDWSLMVYQCCQETTVDEITDDLGELKECVETDGCEEC
                     LGGSSDDPMPSTPCVEDGDCPDDGHCTDGTCYWTTESGDETTTNPTDDIKDDTGDDQT
                     GGEDYTNTAGDGTEEETTAPRKESDGSGCSLVLI"
BASE COUNT          356 a          572 c          563 g          366 t
ORIGIN      
        1 cggtcggccg ggtgtcgttc ggtttggaat acggggggaa caccggcgca tagaaggcga
       61 ggtcggcgcc ggagccgaag gcccgctcga actcacctgt tgtatgggtc gaaacggcga
      121 tggcgaggtc gggcgccgtc gcgcggatcg ccgccacatg atcgagcatc gacgagcgga
      181 ggtgcaggcc gcggacgccg caccggcggg cctgttcggg cgaaccggcg aagaatacct
      241 cgacgccggg acaccgtgcc acgatccgtt cggccgcctt cagcaattcg gcaagagaaa
      301 atacgccgtc attacgaatg gttaccttat gtatgcccgc tgtcgccata cgggcgacgc
      361 tctcgacggt cgcgacgacc gacggggtgc gggtggtgtc ggctatcatc cacagcatgg
      421 aaaggaatct aatcgtggcg ggaaaaatgt caaccggcga aagaagataa cgggaaagaa
      481 ttcagggaga acttgtctag ataagcacca gcgaacagcc agaaccgtcg ctctccttgc
      541 ggggagcggt cgtctcttct tcggtgccgt cgcccgcggt gttggtgtaa tcctcgccgc
      601 cggtctgatc gtcgcccgtg tcatccttga tgtcatcggt cgggttggtg gtggtctcgt
      661 cgccgctctc ggtcgtccag tagcaggtgc cgtcggtgca gtgcccgtcg tcgggacagt
      721 cgccatcctc gacgcacggg gtcgaaggca tcggatcgtc gctactgcca ccgaggcact
      781 cctcacagcc gtcggtctcc acgcattctt taagttcgcc gaggtcatcg gttatctcat
      841 caacggtcgt ttcctgacag cactgataga ccatgagcga ccagtcgccg acccattcac
      901 ccttcgcgat ggcgttgcgc tgttcctcgg tcaatgtcgt ctcgaagcag gtcccctcga
      961 cccattcgac cgcatcaagg caggcgtcga acagttccgc gccgcactcc tctttgaggt
     1021 cgggcgccgt ctcgccgcag ttctcaacga gtttcgccat gcactcatca ctcgtcggca
     1081 ggaagaacgg gggcggcggc acctcgccat caccgtcgcc atcttcgacc ggatcggtcg
     1141 gttcgcccgg ttcggcctca ccccaaccga caccggcgcc ggtgacgcac tgacagtatc
     1201 cgctcgtttc gccgatgacg cagtagcccg cttcgttctc gcaggtcttt tcggtcgcgg
     1261 ggtcgccctc atcgacgcaa tagccgctga agcagaacat cttctcggga caatcctcat
     1321 cgctcacaca ggtcaccgga tcggtatcgg gcacctcccc gtcgccgcag gtcacctgcg
     1381 ggggatacga ttcggccacc gcgcactcgg cggtgccgca gtattcacag ggatcctgat
     1441 cggcgggcca acagaccggc gtcggcatat catcgaactt cacgcaggtc atctgttcgg
     1501 gacagtcgtt cagcgcatcg cactcgatcc acgtctgctc gggcgggtcg gcccgcagta
     1561 tcaccgcgag aaacagggtc atcaggaaca tcaatcctct catcttctcc tccgtcatgg
     1621 ttatgagcga tcgatcaaga actccccttt agtggaaaga agatagcaca tttattgagg
     1681 gagagccagc agaaagatgc cgaacaagga taactgacta taatgacctg gcttattatt
     1741 cctgtatgga gggcgggacg atgaccgtca cgaggcgtga ggagaggtag gacccgttgc
     1801 cgagcaccgt cacgagcgcc tgataggtca tcggattggt cgtcgtgttc ttgcaga
//
LOCUS       DAOQUE010000527         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_81673_length_1856_cov_4.069961, whole genome shotgun sequence.
ACCESSION   DAOQUE010000527 DAOQUE010000000
VERSION     DAOQUE010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81673_length_1856_cov_4.069961"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1072
                     /locus_tag="PKH10_12655"
     CDS             173..1072
                     /locus_tag="PKH10_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29013.1"
                     /translation="MKLFFYAIAASFSVLIFSCASAPKQSGDVTEPSAGDSGGRLISA
                     DPAARPEWLSITGYARQGGKWYFVGESSRSRSDEDARAKALLNGLALVSSQFGVKVSS
                     ELVTREQDSNGNYSYDIGVKNKLTGAPIRIKDYKLDGTYVEKWDRGAVEFDARALIAI
                     PDEEIARIRKEIEALCGWGVLSSRPDLQEDMTRLVKEFAQTKKLNLQPNSVVISDGYS
                     VPALAGQSPDAAFFLVVKVPPVTPKNVDGEWYADVAVTAEYISLTENKVVSSFSASAR
                     GAAYGEAAAVKDGFLKAFQKMMQ"
     gene            1085..1588
                     /locus_tag="PKH10_12660"
     CDS             1085..1588
                     /locus_tag="PKH10_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNT29014.1"
                     /translation="MKHLVMMLLCLTGLTLFAAENDLDFELYNIGDQLYDTKQIRAKP
                     NLKLIAVDFFSIHCEPCKKALPEWRKIYESYKGKGLEIIVVVLPVEDDREKELQKIEL
                     FFKNNPIPFALAYDKYKLVGKKYGVVTKEGSAQVPQAFIIDRAGKLVFKSEGHDEVVK
                     KIKDILK"
BASE COUNT          405 a          500 c          571 g          380 t
ORIGIN      
        1 cgcatcgatg tgtcgctcta cgagatccgt cgcctgacgc aggacaatac cgtgcttgtc
       61 gaagaggtcc accgggccgc ccagcaactc aaggacgaga ccgcgcggct ggtgcgcggg
      121 tgacgaagat acaggaactt tgatgaacat gataggtaag cagaggtctt tcatgaaact
      181 gttcttttat gcgatcgccg cgtcgttctc cgttctgatc ttctcctgcg cctccgcgcc
      241 gaaacagtcg ggcgatgtga ccgagccgtc ggccggcgac tccggtgggc ggcttatcag
      301 cgccgatccc gcggcgcgcc ccgaatggct ttcgatcacc gggtatgccc gtcagggagg
      361 taaatggtat ttcgtcggcg aatcgtcccg atcgcgatcc gacgaggatg cccgggccaa
      421 ggcgctcctc aacggtctgg cgctcgtttc cagccagttc ggcgtgaagg tctcgagcga
      481 acttgtcacg cgggagcagg actcgaacgg caattactcc tacgatatcg gcgtcaagaa
      541 caaactgacc ggcgcgccga tccgcatcaa ggactacaaa ctggacggta cctatgtaga
      601 aaaatgggat cgcggcgccg tcgagttcga tgcccgcgcg ctcatcgcga taccggacga
      661 ggagatagcc cgcatccgca aggagataga ggcgctctgc gggtggggtg tgctttcgtc
      721 gcgtcccgac ctgcaggagg acatgacccg gctcgtcaaa gagttcgcgc agacgaagaa
      781 gctcaatctc cagccgaata gcgtagttat ttctgatggt tactctgtcc ccgccttggc
      841 gggtcagtca ccggatgccg ccttcttcct cgtggtgaag gtgccgccgg tcacccccaa
      901 gaatgtcgat ggtgaatggt atgccgatgt cgccgtgacg gccgaatata tctcgctcac
      961 cgagaacaag gtcgtctctt cgttcagtgc ttcggcgcgg ggggccgcgt acggtgaggc
     1021 ggcggcggtc aaggacgggt tcctgaaggc gttccagaag atgatgcagt aggaggaggg
     1081 atccatgaaa catctggtga tgatgctctt atgcctgacc ggcctgacgc ttttcgccgc
     1141 cgagaacgat ctcgattttg aactttataa catcggggac caactgtacg ataccaagca
     1201 gatacgggcg aaaccgaatc taaaactcat cgcggtcgat ttcttctcga tacactgtga
     1261 gccgtgcaag aaagcgcttc ccgaatggcg gaagatctac gaatcgtaca aggggaaggg
     1321 actcgagatc atcgtcgtgg tcctaccggt ggaggacgac cgcgagaagg aactccagaa
     1381 gatagaactg ttcttcaaga ataacccgat cccctttgcg ctcgcctacg acaagtataa
     1441 gctggtcggg aagaagtacg gtgtggtgac caaggaaggg tcggctcagg taccgcaggc
     1501 cttcatcata gaccgggccg gcaaactcgt gttcaagtcg gaggggcacg acgaggtggt
     1561 caaaaagata aaggatatcc tcaagtgagg gcgcctttga ataaattttg catttcaggg
     1621 tcgaacgggt accatgcgga ggcggtccgc gtttgtttag aaacgacatc cgtgttaggg
     1681 gaggatattc atgctgaatg gtaagagatg gatgggcttt gtcatgctcg gtgtcgcgat
     1741 gttgctgttc gcggccccgc tttcggctca ggaaaaaggc gcgaaaccgc gcatcgcggt
     1801 gctcgacata aatccgtcgg gtgtcccgca aacccttgcg gtctcggtca ccgacc
//
LOCUS       DAOQUE010000528         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_81747_length_1855_cov_4.048333, whole genome shotgun sequence.
ACCESSION   DAOQUE010000528 DAOQUE010000000
VERSION     DAOQUE010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81747_length_1855_cov_4.048333"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PKH10_12665"
     CDS             complement(<1..806)
                     /locus_tag="PKH10_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29015.1"
                     /translation="MRRRILLGALAGTVLFAVVAAILFVKTVYLPARIGAMIDARLSS
                     LPVKVSYDISSVSVLPPAIYFNTVSVARPRGEGALLSLGDCSVSGLKSLLQGKEKALD
                     AVCERGTVDPRQAVELLLAVKEAAGTERSRPDIVKKPPSLSLAVGSLSVIVDDRRLEV
                     DLSADLRGDALNASVRPKGGDVDSFVLLSGSRDNRRLSAVFNRFPAEPYLRPWLPDER
                     TYARALLDGTADLSAESGQAELSIDLSFSEIAISHPFLDVEPFSLPLLRV"
     gene            962..>1855
                     /locus_tag="PKH10_12670"
     CDS             962..>1855
                     /locus_tag="PKH10_12670"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNT29016.1"
                     /translation="MFIYDRVIQEPEKYSFAIDEVGPRTVPTPLKGGLFVADDDQVVF
                     SSQVRNLRKLISSKYFPAFQKAGPREKIFHDPSWSRAAIVTCGGLCPGLNDVIKGLVD
                     VLWWSYGVRTIYGLRYGYQGLSPRYKHAPILLDPDIVDGIHEQGGTILGSSRGNQDVG
                     EMVDTLLRMHINILFCIGGDGTLKGARDIAVEAQKRKQPLSVIGIPKTIDNDLAFVEK
                     TFGYETAVYRTFDIITSAHNEATGAFNGISLVKLMGRDSGFIAAAATLANSVVDFCLV
                     PEIDFELEGETGLLAAIGRCLE"
BASE COUNT          396 a          592 c          541 g          326 t
ORIGIN      
        1 tagacccgca ggagcggcaa cgagaacggc tcgacatcga ggaaggggtg cgatatcgcg
       61 atctccgaga aggaaagatc gatcgaaagc tccgcctgcc cgctttccgc ggagagatcg
      121 gcggtcccgt cgagcaatgc ccgcgcatag gtccgttcgt cggggagcca cggccggaga
      181 tacggttcgg cggggaaccg gttgaacacg gcgctcaggc ggcggttatc gcgggaaccg
      241 gaaaggagca cgaaggaatc cacatcgccg cccttcggtc gcaccgaggc gttcaacgca
      301 tcgccgcgca ggtcggccga cagatcgacc tcgagccgcc ggtcatccac gatgaccgac
      361 aacgatccga ccgccagcga aagtgagggt ggctttttaa cgatatccgg ccgggacctt
      421 tccgttccgg ccgcttcctt caccgcgagg aggagttcga cagcctgccg cggatcgacc
      481 gttccccgtt cacatacggc atccaacgct ttctctttgc cctgcaacag gcttttcagt
      541 cccgaaacgg aacagtcgcc gagcgagagc agggcgccct ccccgcgcgg ccttgcgacg
      601 ctcaccgtgt tgaaatatat cgcgggcggc aggacggata ccgacgagat gtcgtacgac
      661 accttcaccg ggagcgacga gagacgggcg tcgatcatcg ccccgatacg ggcggggaga
      721 tagacggtct tcacaaaaag tatggcggcg accacggcga acagcaccgt gccggcgagc
      781 gcccccagaa gtatccgtcg tctcatgcac cctcatcggt gaaaatagcg cttcattatc
      841 aaagagccaa gacaaaaagc aagaaatagg cccgtcgtcc gtgccgcaaa aaactttatt
      901 tcccgttcat ccagcgatat agtaccatca ttgttcattt atccataggg aggaggcctt
      961 catgttcatc tacgaccgcg tgatacagga accggaaaag tattcgttcg ccatcgatga
     1021 ggtcggcccc cgcaccgtcc cgacacccct caagggcggt ctgttcgtcg ccgacgacga
     1081 tcaggttgtt ttctcaagtc aggtacggaa tctccgtaaa ctcatctcat ccaaatattt
     1141 cccggcgttc cagaaggcgg ggccacgaga gaagatattc catgatcctt cgtggagccg
     1201 ggccgccatc gtcacctgcg gcgggctgtg ccccggactc aacgatgtca tcaagggact
     1261 cgtcgatgtc ctgtggtgga gttacggtgt ccgcaccatc tacggacttc ggtacggcta
     1321 tcaggggctc tcgccgcgct acaagcacgc gccgatcctt ctggaccccg atatcgtgga
     1381 cggcatccac gagcagggag gcaccattct cggctcgtcg cgcggcaatc aggatgtcgg
     1441 cgaaatggtc gacaccctcc tgcggatgca catcaatatc ctgttctgca tcggcggcga
     1501 cggcacgctc aagggcgccc gcgacatcgc ggtcgaggcg cagaagcgca aacaacccct
     1561 cagcgtcatc ggcataccca agacgataga caacgacctc gccttcgtcg agaagacctt
     1621 cggctacgag acggcggtgt accgcacctt cgatatcatc acgagcgccc acaacgaggc
     1681 gaccggcgcc ttcaacggca tatcgctcgt aaaactcatg gggcgcgatt ccggcttcat
     1741 cgcggccgcc gcgacgctcg ccaactcggt cgtcgacttc tgcctcgtcc cggagataga
     1801 cttcgaactc gagggggaga ccggcctgct cgccgccatc ggccgctgtc tggag
//
LOCUS       DAOQUE010000529         1853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_81888_length_1853_cov_4.067297, whole genome shotgun sequence.
ACCESSION   DAOQUE010000529 DAOQUE010000000
VERSION     DAOQUE010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81888_length_1853_cov_4.067297"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PKH10_12675"
     CDS             complement(<1..1076)
                     /locus_tag="PKH10_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29017.1"
                     /translation="MKRFVLFVLVTVLLSFLAGGCSSKEECNADTDCSAGYVCGDDQK
                     CEKLSVTLLFQGLTDGQVLTVADDRDPSDKTIQIDITVAAQANVGSVRDGMPITLEVT
                     RSAAGNDDDALMLRGLTTRTYVGILVNGFAKFLHVPFEEGEHQVRASISHSPDIATPT
                     LTVTATYEVARTVVMRYYKGGTAPDMLEGATLGDSEDLDKEEADLQVKMEAVTTGYPD
                     GQAVEIRIDDHSTGWQTATVQDGVASFASVDVPIWDEITMRVRVGTFEDTLTFSAGSQ
                     QTCGFTMNLENDQVFGKKDDENATLNGLQKTLVISEVTQCTLGSSVKIYINKTPGTDD
                     PDHTLSLGGATLERSITLPESANA"
     gene            complement(1127..1423)
                     /gene="pqqD"
                     /locus_tag="PKH10_12680"
     CDS             complement(1127..1423)
                     /gene="pqqD"
                     /locus_tag="PKH10_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HNT29018.1"
                     /translation="MLDHRYRVRDEIAWRKMKDGTVTIVSPLVEKIISLNATAGIIWE
                     LLDGTRTVAAVIEALEKRFGDEVPRAVLAGDVADILRDLLDRQLIEQFVDNTPH"
     gene            1569..>1853
                     /locus_tag="PKH10_12685"
     CDS             1569..>1853
                     /locus_tag="PKH10_12685"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HNT29019.1"
                     /translation="MALFKRPAPANLTCSVTYRDATLYEETVALLEQVHGPRDAESEP
                     YDFSAISDHYNAEMGGAVMKRIVSFERLFPREHLAEVKRRVTELEQRFSVE"
BASE COUNT          353 a          573 c          537 g          390 t
ORIGIN      
        1 tccgcgttcg cgctctcggg cagggtgatg gaacgctcaa gcgtagcgcc gccgagcgaa
       61 agggtatggt cgggatcgtc ggtgcccggc gtcttgttga tgtatatctt gaccgatgag
      121 ccgagcgtgc actgggtcac ctcgctgatg acgagcgtct tctgcaggcc gttcagcgtc
      181 gcgttctcgt catccttctt gccgaacacc tgatcgttct ccaggttcat cgtgaacccg
      241 caggtctgct gtgaccccgc gctgaaggtg agtgtatcct cgaaggtccc cacccgcacc
      301 cgcatcgtta tctcatccca tatcggcaca tcgaccgacg cgaaggaggc gacgccgtcc
      361 tgcacggtgg cggtctgcca gccggtgctg tggtcgtcga tacgtatctc gaccgcctgc
      421 ccgtcggggt agccggtcgt caccgcctcc atcttcactt ggaggtcggc ctcttccttg
      481 tcaaggtcct cgctgtcacc gagtgtggcg ccctcaagca tgtccggagc ggtgccgcct
      541 ttgtagtaac gcatgaccac cgttctcgcc acctcgtatg tggcggtgac ggtgagggtc
      601 ggtgtcgcga tgtcggggct gtgggagatg gaggcccgca cctgatgctc cccctcctcg
      661 aacgggacat ggaggaactt cgcgaagccg ttcaccagta tcccgacata ggtccgggtc
      721 gtcaggcccc ggagcatgag tgcatcgtcg tcgttccccg cggcggaacg ggtcacttcg
      781 agcgttatgg gcatgccgtc acggaccgac ccgacattcg cctgcgccgc gacggtgatg
      841 tctatctgta tcgtcttgtc cgacgggtcc cgatcgtcgg ccacggtgag cacctgcccg
      901 tcagtgagac cttggaacaa cagggtcacc gacaatttct cacatttctg atcgtcgccg
      961 cagacatatc cggctgaaca atcggtgtca gcgttgcatt cctctttgga ggaacacccg
     1021 cccgcgagga aggacagaag taccgtgaca agcacgaaca acacaaaacg cttcatggct
     1081 ctctccccta tgaccatcta aagaacttca ggcgtcgatg atactttcaa tgcggcgtat
     1141 tgtcaacaaa ttgttctatc aattggcggt ccagaaggtc gcgcaggata tcggcgacat
     1201 cgcccgccag taccgcgcgc ggcacctcgt cgccgaaccg cttctcgagc gcctcgatca
     1261 ccgcggcgac ggtgcgggtc ccatcgagca gttcccagat gataccggcg gtcgcgttga
     1321 gggatatgat cttttcgacg agcggagaca cgatcgtcac cgttccgtcc ttcattttgc
     1381 gccatgcgat ctcgtcgcgg acgcggtacc gatggtcgag catctggggc aacctccctc
     1441 gcgcatcgaa agaacgggtc gattgtatgc acggcgaccg cttttttcaa gaaagagctg
     1501 ttttttgaca aaaaaagtca tctcgataga atccgtgtgt ccatacatgt tacgacacga
     1561 cgggaaccat ggctctattt aaacgaccgg cgccggcgaa cctgacctgc tcggtgacct
     1621 atcgcgacgc cacgctctac gaggaaacgg tcgccctgct cgaacaggtc cacggcccgc
     1681 gcgatgccga aagcgagcca tacgattttt cagcgatatc agaccattac aacgccgaga
     1741 tgggcggtgc ggtgatgaag cgcatcgtct cgttcgaacg actcttcccc cgtgaacacc
     1801 ttgccgaggt gaaacggcgg gtcaccgaac tcgaacagcg cttctcggtg gag
//
LOCUS       DAOQUE010000530         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_82896_length_1839_cov_4.191143, whole genome shotgun sequence.
ACCESSION   DAOQUE010000530 DAOQUE010000000
VERSION     DAOQUE010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82896_length_1839_cov_4.191143"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PKH10_12690"
     CDS             complement(<1..995)
                     /locus_tag="PKH10_12690"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNT29020.1"
                     /translation="MSERKNDKATAKPSLLTGANLYALEIAGAIWALLAAFAWLSLFA
                     YDGTATPAFITGSAGRALAVLLAQVFGYAALLLPLIALFFLLLFFAVFRRAMWRYFLL
                     HLLLFVVAMPLFSFLGLDPLHWGSSGGWIGGKLMNDLMVPHLGKIISILLLVVVLAVY
                     VISTFHVLLFIPLFKGVRSLYEVLKTHLPVILRHLFPEKEEEVPPPVPEESEEEEEQE
                     PMPEETPPAPEPEPRPAKKGKIDVRTIADQKPKKEPTKALKKLPNYELPPLDLLVEEE
                     KSVETRDHETRIQKLAQVIEQKLVQHKIKVSVSGAVIGPVVTMYELDLGEGIRGN"
     gene            complement(973..>1839)
                     /locus_tag="PKH10_12695"
     CDS             complement(973..>1839)
                     /locus_tag="PKH10_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29021.1"
                     /translation="LRLAGWEKSSARTMVGRDPRITFRRSVLNLPFGEETLTAFFYSL
                     RDSIRHPGSLAALYHPAFSGTPVTVRVNLFSVELLLGDPVLLSPEGLFLWFYTLNTPT
                     DRYDEEFYLNLLALKLAKRLYTGLPSFLGGASLFTHSGEIPFDQMRKSLFSKDAIETT
                     GFSFPYSYVWSGELFTETTGTLPIERAEPTQAFYGATEGADRALCVIVLRAADADEKR
                     DAVETLLRTKGFTPLLRLHEWTGEQDGWAALGFVVPAAQESVIMRIFETELAKMDRTI
                     SIGVYRVGTQER"
BASE COUNT          355 a          517 c          605 g          362 t
ORIGIN      
        1 tgattgcccc ggatcccttc gccgaggtcg agttcgtaca tcgtgacgac cggtccgatg
       61 accgcgccgc tcacgctcac ctttatcttg tgctggacaa gtttctgctc gatgacctgc
      121 gccaacttct ggatgcgcgt ctcgtggtcg cgggtctcga cgctcttctc ctcctcgacg
      181 agcaggtcga gtggcggcag ttcgtagttc gggagtttct tgagcgcctt ggtcggttcc
      241 ttcttcggtt tctggtcggc gatggtacgc acatctattt tgcctttctt cgccggtctc
      301 ggttcgggtt ctggtgcggg aggtgtttct tccggcatcg gttcctgttc ctcctcctcc
      361 tcgctctctt ccgggaccgg cggcgggacc tcttcctcct tttcggggaa gagatgccgg
      421 aggatcaccg gtaggtgggt cttaagcacc tcgtagagag agcgcacccc cttgaagagc
      481 ggtatgaaga gcagtacatg gaaggtcgat atcacataga ccgcgagtac gacgacgagc
      541 aacaggatgc tgatgatctt tcccagatgg gggaccatga ggtcgttcat cagtttgccg
      601 cctatccagc caccgctcga tccccaatgg agcggatcga ggcccagaaa ggaaaaaagc
      661 ggcatcgcga cgacgaaaag cagaaggtgc aggaggaagt atcgccacat cgctcgccgg
      721 aataccgcga agaaaaggag caggaaaaag agcgcgataa gcgggagaag cagggcggcg
      781 tagccgaaca cctgcgcgag gagcaccgcc agggcgcggc ccgccgagcc ggtgatgaag
      841 gccggggtcg ccgtgccgtc gtaggcgaag agagaaagcc aggcgaaggc ggcgaggagc
      901 gcccagatcg caccggcgat ctcgagggcg tagaggttcg cgccggtcag gagtgacggc
      961 tttgccgttg ccttatcgtt cttgcgttcc gacacggtag actcctatgg atatggttcg
     1021 gtccatcttt gccagttcgg tctcgaagat cctcatgatg acgctttcct gcgccgcggg
     1081 cacgacgaac ccgagcgcgg cccacccgtc ctgctcgccg gtccattcat gaagccggag
     1141 gaggggcgtg aagcccttcg tcctgagcag ggtctcgacg gcatcccgct tttcatcggc
     1201 atcggcggcg cgcagaacga tgacacagag cgcgcggtcc gcaccctcgg tggcgccata
     1261 gaaggcctgt gtcggttcgg cccgttcaat ggggagggtg ccggtcgtct cagtgaacag
     1321 ttctccgctc cagacgtagg agtaggggaa cgagaacccg gtcgtctcga tcgcgtcctt
     1381 cgaaaagagg cttttccgca tctggtcgaa cggtatctcg ccgctgtggg tgaaaagcga
     1441 tgcgccaccg agaaaggagg ggagtccggt gtaaagccgt ttggccagct tgagcgcgag
     1501 caggttgagg tagaactctt cgtcatagcg gtcggtgggg gtgttgagcg tatagaacca
     1561 gaggaagagt ccttcgggtg acaggaggac cggatcgccg agcaggagtt ccaccgaaaa
     1621 gagattgacc cgcaccgtta ccggggtgcc cgagaaggct ggatggtaga gcgccgcaag
     1681 actgccgggg tggcgtatcg aatcgcgtaa actgtaaaag aacgcggtca gcgtctcctc
     1741 gccgaacggc aggttgagta ccgatcgcct gaaggttatg cgcgggtccc tgccgaccat
     1801 cgtgcgcgca gaactttttt cccatccggc gagacgcag
//
LOCUS       DAOQUE010000531         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_83560_length_1830_cov_3.166197, whole genome shotgun sequence.
ACCESSION   DAOQUE010000531 DAOQUE010000000
VERSION     DAOQUE010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83560_length_1830_cov_3.166197"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PKH10_12700"
     CDS             complement(<1..898)
                     /locus_tag="PKH10_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNT29022.1"
                     /translation="MRPFVTLLCAMLIATACSSNQKGDDAMDQKLQKLPPIIPREVLF
                     GNPVKAAVRLSPDGTRLSYFAPSPDKNVLNVWVRTLGQNDDKMVTKDEYRGIRSYGWA
                     WNDRQIVYIQDLNGDENFHLYAVDLATLETKDLTPFPGVRAQGIISDRKHPDEIYIGL
                     NKRDPQVFDIYKLNLVTGELTLDVQNPGDVEGWLTDRDFVIRAAVASNLETADTILRI
                     RDGKDAPWRDFMTIPFGENGSIVDFTENNSTLLIETSVGGDTTRLIEYDPVEGKELRL
                     IAQNPKADVGGIMLHPETNAVQA"
     gene            1033..1382
                     /gene="ssrA"
                     /locus_tag="PKH10_12705"
     tmRNA           1033..1382
                     /gene="ssrA"
                     /locus_tag="PKH10_12705"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1404..>1830
                     /locus_tag="PKH10_12710"
     CDS             1404..>1830
                     /locus_tag="PKH10_12710"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNT29023.1"
                     /translation="MHKVTLLPDKDKLPRNRHPWVFSGAVKDIEGAPATGDIIALRDA
                     EGKFIAYGHFNKDSKIQVRLLSWDESRIPDEGWIRERIRDAVRDRAAILRDGATDTCR
                     LIYSEGDLLPGLIVDRFGDHAVLQALSAGFDRIKEAAARI"
BASE COUNT          349 a          540 c          575 g          366 t
ORIGIN      
        1 ccgcctgtac cgcgttggtc tcgggatgga gcatgatgcc tcccacatcg gccttcgggt
       61 tctgggcgat gagccgcagt tccttccctt cgaccgggtc gtattcgatg agccgcgtgg
      121 tgtcgccgcc gaccgaggtc tcgatgagga gcgtactatt gttctcggtg aaatcgacga
      181 tcgagccgtt ctcgccgaag gggatggtca tgaagtcgcg ccacggggca tccttgccgt
      241 cgcggatgcg gagtatcgtg tcggctgtct cgaggttcga ggcgaccgcg gcgcggatca
      301 cgaaatcgcg gtcggtgagc cacccctcga catcgcccgg attctggaca tcgagcgtca
      361 gttcacccgt cacgaggttg agtttgtata tgtcgaagac ctgcgggtcg cgcttgttga
      421 gcccgatgta tatctcgtcg ggatgcttgc ggtcactgat gatcccctgc gcccggacgc
      481 cggggaaggg ggtcaggtcc ttcgtttcga gcgtcgccag atcgaccgcg tagaggtgga
      541 agttctcatc gccgttcaag tcctgaatgt agacgatctg cctgtcgttc cacgcccagc
      601 cgtaactgcg gatgccgcga tactcgtcct tggtgaccat cttgtcgtca ttctggccga
      661 gtgtccgcac ccagacattg agcacattct tgtcggggct cggggcgaag tacgacagcc
      721 gcgtgccgtc gggcgacagg cggacggcag ccttgacggg attgccgaaa agtacctccc
      781 gggggatgat cgggggcagt ttctgtaact tctggtccat ggcgtcatct cctttctggt
      841 ttgacgaaca ggccgtggcg atgagcatcg cgcagagcag ggtcacaaac gggcgcatga
      901 tatcctccca agtggtccat gtaccgcgac atcgtaacgc gtcggtccga taagtcaact
      961 gaaaggagga ggcgtttgac aaagcgcttt gccgcagtat actcaccggt gtaccatcgt
     1021 tttttgacaa gtggggacga acggcttcga cgggaagacg gagcgttgca gactgcatgc
     1081 cgaggcgccg ctggcctcgt aaaaagcggc aacacaagaa ctgcgaacaa cgaactcgca
     1141 ctagcggctt aattgccgcc gtcctgatgg atctctcccg cggtgagatc agggcgcaaa
     1201 aaagcgggat agtgccgtga ccgggtcaca aggtcgcggc tgacacaaaa gtgactgggc
     1261 cgatgcgatc catgccggta cggggagcgc aaaggccgag aataaaccaa ccggatacgc
     1321 actgtagtat gtcggcatct ctcccttttc ggacgcgggt tcgattcccg ccgtctccac
     1381 catttttccc cgaggtattc tccatgcaca aggtcacgct tctccccgac aaggacaaac
     1441 tgccgcgcaa ccgccacccg tgggtcttct cgggcgcggt gaaggatatc gagggtgccc
     1501 cggcgacggg cgatatcatc gcgctccggg acgccgaagg gaagttcatc gcctacggcc
     1561 atttcaacaa ggattccaag atacaggtgc gtctcctttc gtgggacgag tcgcgcatcc
     1621 ccgacgaagg gtggatccgt gaaaggatac gcgacgcggt ccgcgatcgc gccgccattc
     1681 tgcgggacgg cgccactgac acctgccggc ttatctacag cgaaggcgat ctcctgccgg
     1741 gactcatcgt cgaccggttc ggcgaccatg cggtcctgca ggcgctgtcg gcgggattcg
     1801 accgcatcaa agaggcggca gcccgcatcc
//
LOCUS       DAOQUE010000532         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_84567_length_1817_cov_4.063564, whole genome shotgun sequence.
ACCESSION   DAOQUE010000532 DAOQUE010000000
VERSION     DAOQUE010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84567_length_1817_cov_4.063564"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..572
                     /locus_tag="PKH10_12715"
     CDS             333..572
                     /locus_tag="PKH10_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29024.1"
                     /translation="MRKMTAFLAAMVMVLVFGCASTPEAAPAPAAEPAPAAEPAPAAE
                     PAPAAEPAPAAEAAPAEAAPAETPAEAPAEAPAAE"
     gene            complement(626..1675)
                     /locus_tag="PKH10_12720"
     CDS             complement(626..1675)
                     /locus_tag="PKH10_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29025.1"
                     /translation="MKRTGLWALALLTLLTACGEEKKEQAPLEIIGTYEDGFGYTHIV
                     TETAWDQGDMGLFHITKYDNENDFLVAQNDTVKSYNPGKWSRMDWVWHAGALYVCQIA
                     YDKDSAAAAEAETGADPTDIEKGCNENPWSELTVKTFTGAAGAAGSPAVAKDDPRIVA
                     WATGIAAYEPGENLTEQWKDTANAIGPAEGNASAIVSLGEGGSITLTFESPITDGAGY
                     DIAIFENSFVDDFLELAFVEVSSNGEDFVRFDATYLGAGPIGEYGTLAPSLIDGLAGK
                     YRAGWGTPFDLATLAGNAAVTAGTIDLSAITHVRIVDIKGDGAMTDSHGHAIYDPYPT
                     RESAGFDLDAVGQIN"
BASE COUNT          275 a          556 c          564 g          422 t
ORIGIN      
        1 acggcgagat ggctaccacc gacaccgctc ccgcgcccga ggcggctcct gctgaggcgg
       61 ctcccgccga ggcggctccc gccgaagaag cgccggccga ggctcccgcc gaggctcccg
      121 ccgaggctcc cgccgaggct cccgccgagt aagcgatact tcttgctttc acccttcctg
      181 ttaagatcct gaaaaatgtc tctccgtttt attttctttt ctttagtgga aaaagttgct
      241 ttatgtttcg aaaagcagta gataggatgt catgttttgg gacggtaacg gtccttgctc
      301 ttttagtttt acctgtaaaa ggaggttaga tcatgcggaa gatgacggcg ttcttggcgg
      361 cgatggttat ggtccttgtg ttcggttgcg ccagcactcc cgaagcggcc cccgctccgg
      421 cggctgagcc cgctccggcg gctgagcctg ctccggcggc tgagcccgct ccggcggctg
      481 agcccgctcc ggcggctgag gcggctccgg ctgaggcggc tccggctgag actcctgctg
      541 aggctccggc cgaggctccc gcggctgaat aagccatccc cgttccattt tttcgccccc
      601 gcttttgcgg gggttttttt tcggttcagt tgatctgtcc gaccgcgtcg aggtcgaacc
      661 cggcgctctc ccgtgtcggg taggggtcgt atatggcgtg accgtgactg tcggtcatcg
      721 cgccgtctcc tttgatatct acgatacgga catgggttat tgccgacagg tcgatcgttc
      781 cggcggtcac ggcggcgttc ccggcaagcg tcgcgagatc gaacggggta ccccatccgg
      841 cgcgatattt cccggcaaga ccgtcgatga gcgacggggc gagcgtgccg tactcgccga
      901 tcgggccggc gccgagatag gtggcgtcga atcgaacgaa atcctcaccg ttgcttgata
      961 cctcgacgaa ggcgagttcg aggaagtcgt cgacgaagga attctcgaat atcgcgatgt
     1021 catagcccgc gccgtcggtg atggggcttt cgaaggtcag tgttatgctg ccgccctcgc
     1081 cgagcgaaac gatggcgctc gcgttccctt cagcggggcc gatcgcgttt gccgtatctt
     1141 tccactgttc ggtcaggttt tctcccggct cgtaagcggc gatgccggtc gcccacgcga
     1201 cgatgcgcgg gtcatctttc gcgacggcgg gggagccggc ggccccggcc gcccccgtaa
     1261 aggtcttgac ggtaagttcg ctccacggat tctcgttgca tcccttctcg atgtcggtcg
     1321 ggtctgcgcc ggtctccgcc tcggccgccg ccgccgagtc tttgtcatat gctatctggc
     1381 agacatagag agcaccggcg tgccagaccc agtccatccg gctccacttg ccggggttgt
     1441 aacttttcac cgtatcgttc tgcgcaacga gaaaatcgtt ttcgttgtcg tacttcgtga
     1501 tatggaacaa gcccatatcg ccttgatccc atgccgtctc ggttacgatg tgggtgtagc
     1561 cgaaaccgtc ctcgtaggtg ccgatgatct cgagcggcgc ctgttctttc ttctcctcgc
     1621 cgcaggcggt aaggagcgtc aaaagggcga gcgcccaaag gccggtgcgt ttcatggtcg
     1681 ttctccgttc tctgtggctg ttatgtcccg tatccatatc cggatcgccg cgggagcatc
     1741 agggatagat atcgtcgtcg ttgttcccgt cgcggtgtcg agcgcgttga gtcggctgaa
     1801 gatcgcgccg ttctcgg
//
LOCUS       DAOQUE010000533         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_84572_length_1817_cov_3.822361, whole genome shotgun sequence.
ACCESSION   DAOQUE010000533 DAOQUE010000000
VERSION     DAOQUE010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84572_length_1817_cov_3.822361"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKH10_12725"
     CDS             complement(<1..506)
                     /locus_tag="PKH10_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNT29026.1"
                     /translation="MTDLRVHIGATTLKNPILTASGTFGYGPEFEPYLNLHALGGFCT
                     KGISLEPRGGNPGIRIVETPSGMLNSIGLENCGSRAFLDEMLPKIAAYDTAIIVNFYA
                     HSEEDFVKLAATLSESDRIDALEMNLSCPNVKQGGSAFGADPRLIESLTAAVRKVTKK
                     TLIVKLSP"
     gene            complement(503..1261)
                     /locus_tag="PKH10_12730"
     CDS             complement(503..1261)
                     /locus_tag="PKH10_12730"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNT29027.1"
                     /translation="MQRSIVTAIADMMPVADDCFLMAVPFEPEPLPGQFVMLSFGDGR
                     TDPFLPRPISIFDWEGGMLTLLIRVVGRGTLLLGRQVAGATVRVTGPLGNSFPDDAGR
                     VCFIGGGIGVAPLFYAAKRSKAKDRSFILGFRSADESYLTGEFSEVAPVRFVSDDGSL
                     GEKLFPHEKLDALVTAGERPDLIVTCGPEKMMAAVHGVAKKHGLRDVHSLESRMGCGI
                     GACLGCRVSMPGASVLVCKEGAVFDGSVFDGEKG"
     gene            complement(1246..1692)
                     /gene="rimI"
                     /locus_tag="PKH10_12735"
     CDS             complement(1246..1692)
                     /gene="rimI"
                     /locus_tag="PKH10_12735"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNT29028.1"
                     /translation="MRDHIEELLSIERESFADPKNGNIFSSLYDDAASHMHYFASRDA
                     AGGIDGYLLLSLAADEAEIVNVAVRPSRRREGVGSALVGEAIDFARSRGAVACYLEVR
                     ESNGAARRLYEKMSFNVVGVRKMYYTAPPEDAIVMRRALEAACNDR"
BASE COUNT          326 a          589 c          577 g          325 t
ORIGIN      
        1 ttgggcgaga gtttcacgat aagcgtcttt ttcgtcacct tgcggaccgc ggcggtgagc
       61 gactcgatga ggcgcggatc ggccccgaag gcgctgccgc cctgcttgac attggggcac
      121 gagaggttca tctcgagcgc gtcgatgcgg tcgctttccg acagcgtggc ggcgagtttc
      181 acgaaatcct cttcgctgtg ggcgtagaaa ttcactatga tggcggtgtc atacgcggct
      241 atcttcggca acatctcgtc gaggaacgcg cgcgacccgc agttctccag tcctatcgaa
      301 ttgagcatgc ccgacggggt ctcgacgatg cggatgcccg gattgccccc gcgcggctca
      361 agcgatatcc ccttcgtgca gaagccgccg agagcgtgca ggttgaggta cggctcgaac
      421 tcgggaccgt agccgaaggt ccccgaggcg gtcagtatcg ggttcttgag cgtcgtggcg
      481 ccgatgtgca cgcgcaggtc ggtcatcctt tttccccatc gaagaccgag ccgtcgaaca
      541 ccgccccctc cttgcagacg agtaccgagg cgcccggcat gctgacgcgg cacccgagac
      601 aggcaccgat gccgcacccc atgcgcgact cgagcgaatg gacatcgcgc aggccgtgct
      661 ttttcgccac cccgtgcacc gccgccatca tcttctcggg accgcaggtg acgatgaggt
      721 ccggtctttc gcccgccgta acgagcgcgt cgagtttctc gtgcgggaac agtttttcgc
      781 ccagagagcc gtcgtcgctc acgaaccgga ccggcgcgac ctccgagaac tcgccggtga
      841 gataactttc gtcggctgac cggaagccga gtatgaacga ccggtccttg gccttcgatc
      901 gcttcgcggc atagaaaagc ggcgcgacgc cgatgccgcc gccgatgaag cacacccgcc
      961 ccgcgtcgtc agggaacgag ttgccgaggg gtccggtcac ccgcaccgtc gctccggcca
     1021 cctgtcgccc taacagcaga gtgccgcgac cgacaacacg gatgaggagc gtgagcatgc
     1081 ccccctccca atcgaagatt gagatgggac gggggaggaa cggatcggta cgaccgtcac
     1141 cgaacgaaag catgacgaac tgtccgggga gcggttcggg ctcgaagggg accgccatga
     1201 ggaagcagtc gtccgcgacc ggcatcatat cggctatggc ggtgactatc gatcgttgca
     1261 cgcggcctcc agtgcccggc gcatcacgat ggcgtcctcg ggcggggcag tataatacat
     1321 ttttctcacg cccacaacat taaacgacat tttttcgtac agacgccgcg ccgcgccgtt
     1381 gctctcgcgc acttcgaggt agcaggcgac cgcgccccgg gagcgcgcga agtcgatcgc
     1441 ctccccgacg agcgccgatc cgacgccctc ccgccggcga gaggggcgga ccgcgacatt
     1501 gacgatctcc gcctcgtcgg ccgcgagcga cagcaggaga tacccgtcga tgccgcccgc
     1561 tgcgtcccgc gaggcgaaat agtgcatatg cgacgccgcg tcgtcgtaga gcgaactgaa
     1621 gatgttgccg ttcttcggat cggcgaaact ctcgcgttcg atggagagca gttcttcgat
     1681 gtgatcgcgc atgaagcggc ggtcggcctg caccgcggtc ctccgccgga acgccggctt
     1741 acttgaggtt ctcggggttg agccccttca gttccttcat gacgaacagg ccgtccttgc
     1801 ggatgagttt gccgtcg
//
LOCUS       DAOQUE010000534         1815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_84733_length_1815_cov_4.144318, whole genome shotgun sequence.
ACCESSION   DAOQUE010000534 DAOQUE010000000
VERSION     DAOQUE010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84733_length_1815_cov_4.144318"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /gene="groEL"
                     /locus_tag="PKH10_12740"
     CDS             complement(<1..1006)
                     /gene="groEL"
                     /locus_tag="PKH10_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNT29029.1"
                     /translation="MAKEIYFEQNAKQSILRGVDVLANAVKITLGPKGRNVVLEKSYG
                     APKITKDGVTVAKEISLEDKVENLGAQLVKEVANKTSETAGDGTTTATVLAQAIIREG
                     YKYITAGYSPMGIKRGIDKAVGHIVGELDKKTKKVNGFEDIMSVGTVSANNDKEIGKI
                     IAEAMEKVGKEGVITVEEAKGMETTLDTVEELQFDKGYISPYFITDAEKMKAELEEPY
                     ILVFDKKVTNLKDIVPLLEGVARAGKPLLIISEDVEGEALATLVVNKLRGILKIAAVK
                     APGFGDRRKAMLEDIAIVTGGQFISEELGHKLEGVTIDKLGKAKRVIIDKENTTIVDG
                     A"
     gene            complement(1022..1312)
                     /gene="groES"
                     /locus_tag="PKH10_12745"
     CDS             complement(1022..1312)
                     /gene="groES"
                     /locus_tag="PKH10_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNT29030.1"
                     /translation="MKLRPLNDRVIVRRKSGEEKTKGGIIIPDTAKEKPIEAEVVAVG
                     EGKLLDNGKKEPVGVKAGDTVLFGKYSGTEVKIDGEEYLILRGEDILAVVEK"
     gene            complement(1449..>1815)
                     /locus_tag="PKH10_12750"
     CDS             complement(1449..>1815)
                     /locus_tag="PKH10_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29031.1"
                     /translation="GEFIVSITGEEIPDYEYPDDDAFTDALIGDEDDLLTDEMSDPDA
                     VADDGQPTDHDTVVDVDQWQPDADQTPTDDQPVDKDASVNDTDDVQSDADELLTDQSG
                     DDSEPNRPKDDGCGCSLVF"
BASE COUNT          300 a          549 c          506 g          460 t
ORIGIN      
        1 cggcgccgtc gacgatggtg gtgttctcct tgtcgatgat gacccgtttg gccttgccga
       61 gcttatcgat ggtcacgccc tcgagtttat ggcccagttc ctcgctgatg aactgaccgc
      121 cggtcacgat ggcgatatcc tcaagcatgg ccttgcgtcg gtcgccgaaa ccgggcgcct
      181 tgaccgcggc gatcttgagg atgccgcgca gtttgttgac gacgagcgtc gctagtgcct
      241 cgccctcgac atcctcggag atgatgagga gaggtttgcc ggcgcgggcc acgccctcga
      301 gcaggggcac gatgtcctta aggttggtca cttttttatc gaacacgagg atgtagggtt
      361 cctcgagttc cgccttcatc ttctcggcgt cggtaatgaa gtagggagag atatagcctt
      421 tgtcgaactg gagctcttca acggtatcga gcgtggtctc catgcccttc gcctcctcca
      481 ccgtgatgac cccctccttg ccgaccttct ccatcgcctc ggcgatgatc ttgcctatct
      541 ccttgtcgtt gttcgccgac acggtgccga ccgacatgat atcctcgaac ccgttgacct
      601 ttttggtctt tttgtcaagt tcgccgacga tgtggcctac cgccttgtcg atgccgcgct
      661 tgatgcccat cgggctgtag ccggcggtga tgtacttgta accctcgcgg atgatcgcct
      721 gcgcgagcac cgtcgcggtg gtggtgccgt caccggcggt ctctgaggtc ttattggcga
      781 cctccttgac cagttgggcg ccgaggttct cgaccttatc ctcgagcgat atctccttgg
      841 cgacggtcac gccgtccttg gttatcttgg gagcgccata actcttttcg agcacgacat
      901 tgcgcccctt ggggccgagc gttatcttga ccgcgttggc gagcacatcg acgccgcgca
      961 ggatcgattg cttcgcattc tgttcgaaat agatctcttt cgccatggta tccctccgta
     1021 gttacttttc aacgaccgca aggatatcct cgccccgcag gatgagatac tcctcgccgt
     1081 ctatcttgac ctcggtgccg ctgtacttgc cgaacagcac cgtgtcgccc gcctttacgc
     1141 cgaccggctc ttttttgccg ttgtcgagga gcttcccctc gcccacggcg accacttcgg
     1201 cctctatcgg cttttcttta gcggtgtcgg ggatgatgat gccccctttg gtcttctctt
     1261 cgcccgattt ccttctcacg atcacccgat cgttcaacgg tctcagtttc atggacacct
     1321 cctttgtcaa aatgaaagtc caaagacggg aaggagaata agcaccgttc gggacaagtc
     1381 aagggaacga gggggttttt gacgaagtct tggaaaagac gccgaatttc ccttcagaaa
     1441 ttagaaagtt agaagaccaa agaacagccg cacccgtcgt ctttaggccg gttcggttcg
     1501 ctgtcgtcac cgctctggtc ggtcagcagt tcatcggcat cgctctgaac atcgtcggtg
     1561 tcgttcacgg aagcatcctt atcgaccggt tggtcgtcgg tcggcgtctg gtcggcgtcc
     1621 ggctgccact ggtcgacatc gacgaccgtg tcgtggtcgg tcggctggcc gtcatcggcg
     1681 acggcgtcgg gatcgctcat ctcgtcggtc agcaggtcat cctcatcgcc gatcaaggca
     1741 tcggtgaacg catcgtcatc ggggtactcg taatcgggta tctcctcccc ggtgatggat
     1801 actatgaact cgccc
//
LOCUS       DAOQUE010000535         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_84956_length_1812_cov_3.782584, whole genome shotgun sequence.
ACCESSION   DAOQUE010000535 DAOQUE010000000
VERSION     DAOQUE010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84956_length_1812_cov_3.782584"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKH10_12755"
     CDS             complement(<1..312)
                     /locus_tag="PKH10_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNT29032.1"
                     /translation="MGNNPATGPLTGVKTRDEARTKEPSLYRVLLHNDDFTTMDFVVQ
                     ILVQVFQRSAAEATQLMLDVHHKGVGTAGVYTYDIAMSKVIQAHQLAEEHRFPLRCTV
                     EK"
     gene            complement(396..>1812)
                     /gene="aspS"
                     /locus_tag="PKH10_12760"
     CDS             complement(396..>1812)
                     /gene="aspS"
                     /locus_tag="PKH10_12760"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNT29033.1"
                     /translation="PYDHRKEAADTLKLKYRYLDIRKSDLLPTLVARSKMAKAVRDYM
                     DANAFVEVETPILGKSTPEGAKEYLVPSRLHHGLCYSLPQSPQSFKQICMIGGIDRYY
                     QMAKCFRDEDLRADRQPEFTQIDIEMSFIAEEDIMTLSEGLIAHLFKVVKGIDIPRPF
                     KRLPFREAMDRYGSDKPDTRYGLEFIDMRENFRNSGFNAFKGAAEDPKKSVKAIVLEG
                     KGAELSKNRIKAMEKDVQGDGAKGLAWVKKEGGALDSPLLKFFSPVELEKLAALIPDG
                     AVMFLVADETRIVQTALGNLRRRLAAEFDLYDKNGFAFLWVTEFPAFEHSEEEGKWVA
                     THHPFTDFDLETARKGDMANVSSRAYDMVINGYEVGGGSIRIHNTAKKAEVFKLLGIE
                     PQEAQEKFGFLLEALDLGAPPHGGIAFGFDRLVMILLGIDNIRSVIAFPKTTSASCLM
                     TGSPSGVSQKQLDELAIKPVG"
BASE COUNT          317 a          539 c          554 g          402 t
ORIGIN      
        1 cttttcgacg gtacagcgga gcgggaatcg gtgttcctcc gccagttgat gcgcctgtat
       61 caccttggac atcgcgatat cgtaggtgta gacgcccgcg gtgccgaccc ccttgtggtg
      121 aacatcgagc atcagttgcg tcgcctcggc cgccgaccgc tggaacacct gcacgagtat
      181 ctgtaccacg aagtccatcg tcgtgaagtc gtcgttgtgc agaagaacgc ggtagagcga
      241 cggctctttc gtccgcgcct cgtcgcgggt ctttactccg gtcagcggac cggtcgccgg
      301 gttgttcccc atggtcaggg catcctcctt tttaacggtg gtatcatagc gaatagcgtc
      361 gaaatgtaaa gggaaccgtt atgcggcgcc gacggtcagc cgaccggctt gatggccagt
      421 tcgtcgagtt gtttctgcga cacgcccgat ggactgccgg tcatgagaca actggcgctc
      481 gtcgtcttcg ggaaagcgat gaccgaacgg atattgtcga tgccgagcag gatcatcacg
      541 agccggtcga acccgaaggc gataccgccg tgcggcggtg cgccgagatc gagcgcttcc
      601 agcaggaacc cgaacttttc ctgcgcttcc tgcggttcga tgccgagcag tttgaacacc
      661 tcggccttct tggcggtatt gtggatgcgg atggagccgc cacccacctc gtaaccgttt
      721 atcaccatat cataggcgcg gctcgacaca ttcgccatgt cgccctttcg cgccgtttcg
      781 aggtcaaagt cggtgaaggg atggtgtgtc gcgacccatt tcccctcttc ctcgctatgt
      841 tcgaacgcgg ggaattcggt gacccagagg aaggcgaagc cgttcttgtc gtagagatcg
      901 aattcggccg cgagccgccg ccgcaggttg ccgagcgccg tctggacgat gcgtgtctcg
      961 tcggcgacga ggaacatcac cgcgccgtcg gggatgagtg ccgcgagttt ctccagttcg
     1021 acgggcgaaa agaacttgag gagcgggctg tcgagcgcgc cgccctcctt cttcacccac
     1081 gccagcccct ttgcgccgtc gccctgaaca tccttttcca tcgccttgat gcggttcttg
     1141 gaaagttcgg cccccttccc ttccaacacg atggccttga cgcttttttt cgggtcctcg
     1201 gccgctccct tgaaagcgtt gaacccggag ttccggaagt tctctctcat atcgatgaac
     1261 tcgagtccgt aacgggtatc gggcttgtcg gagccgtagc gatccatcgc ctcgcggaac
     1321 ggcagccgtt tgaagggacg cggtatgtcg atacccttca ccaccttgaa gagatgggcg
     1381 atgagccctt cggacagcgt catgatatcc tcttcggcga tgaaggacat ctcgatgtct
     1441 atctgggtga attcgggctg gcggtcggcg cgcaggtcct cgtcgcgaaa acacttggcc
     1501 atctggtagt aacggtcgat gccgccgatc atgcatatct gcttgaacga ctgcggcgac
     1561 tgcggcaacg agtagcacag cccgtggtgc aggcggctcg ggacgagata ttccttggcc
     1621 ccctccggcg ttgacttgcc gagtatcggc gtttcgacct cgacgaaggc gttggcatcc
     1681 atatagtcgc gcaccgcctt ggccatcttc gagcgggcga ccagcgtcgg gagcaggtcc
     1741 gacttgcgga tatcgaggta acggtatttg agtttcagcg tgtcggccgc ctctttgcga
     1801 tggtcgtagg gg
//
LOCUS       DAOQUE010000536         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_85123_length_1810_cov_4.016524, whole genome shotgun sequence.
ACCESSION   DAOQUE010000536 DAOQUE010000000
VERSION     DAOQUE010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85123_length_1810_cov_4.016524"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PKH10_12765"
     CDS             complement(<1..1011)
                     /locus_tag="PKH10_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNT29034.1"
                     /translation="MKDPRIEKLAKNLVRFSCRVKKGEHVLIEVKDESAKPLVEALVE
                     EVQAVGATPFVNLYDQRIMRKLVGGASEAWFKIMAEVDLLKMKKMHAFIGINGRNNSY
                     EFSDVPGAQMKSYMQKYVQPVHFEQRVKRTKWCILAYPNPSYAQGSNMSTEAFENFYF
                     DVTTLDYAKMDKAMDPLVKLMDRTDKVHLKGPGRTDLTFSIQGIPTIKCAGQMNIPDG
                     EIFTAPVKDSVNGIIEYNTPTVYLGKKFEKVVLTFKKGKIVKVEGDNVPKLEEIFNSD
                     EGARYVGEFAIGVNPYITKPMVDILFDEKIAGSIHFTPGNSYDEAPNGNSSQVHWDLV
                     MIQ"
     gene            complement(1094..1615)
                     /locus_tag="PKH10_12770"
     CDS             complement(1094..1615)
                     /locus_tag="PKH10_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29035.1"
                     /translation="MQVVTVHHLKKARRSLYIDEAPFRSRSPVALTRPLEGIPKRRKG
                     LVATLPDIAQASGLGQYLRLSGVVSVGPFTVEPAWVGDRRMNLLIDGAWYYLSAVPDT
                     PFTPRCATTLLFPADTAPDRSSLAAFTRFVRGSGFAAVLASGAHAAAWRDRCAASFPI
                     DVRERPAQLPIDW"
BASE COUNT          305 a          552 c          588 g          365 t
ORIGIN      
        1 ctgtatcatg acgagatccc agtggacctg actgctgttc ccgttcggcg cctcgtcgta
       61 ggaattgccg ggggtgaagt gtatcgagcc ggcgatcttc tcgtcgaaca ggatgtcgac
      121 catcggcttg gtgatatagg gattgacccc tatcgcgaac tcgccgacat agcgggcgcc
      181 ttcgtcgctg ttgaatatct cctcgagttt gggcacattg tcgccctcca ccttcacgat
      241 cttccccttc ttgaaggtaa ggacgacctt ctcgaacttt ttgccgagat agacggtcgg
      301 ggtgttgtac tcgatgatgc cgttgaccga atccttcacc ggcgcagtga agatctcgcc
      361 gtcggggatg ttcatctggc cggcgcactt gatggtgggg atgccctgta tcgagaaggt
      421 gagatcggtg cggccgggac ctttgaggtg taccttgtcg gtcctgtcca tcagtttcac
      481 gagcgggtcc atcgccttgt ccattttggc gtagtccagc gtcgtgacat cgaaataaaa
      541 gttctcgaac gcctcggtcg acatgttgga accctgcgcg taagaggggt tcggataggc
      601 gaggatgcac catttggtcc gcttgacgcg ctgttcgaaa tggaccggct gaacatattt
      661 ctgcatgtaa ctcttcatct gagcgcccgg cacatcggag aactcgtagg agttgttgcg
      721 accgttgatg cctatgaagg cgtgcatctt cttcatcttc aggagatcga cctcggccat
      781 gatcttgaac cacgcctcgc tcgccccgcc gaccagtttc cgcatgatgc gctggtcgta
      841 caggttcacg aacggcgtcg cgccgaccgc ctgcacctct tcgacgagcg cctcgacgag
      901 cggttttgcc gactcatcct tcacctcgat gaggacatgc tcgccctttt tcacgcggca
      961 ggaaaaacga accaggttct tcgccagttt ctcaatgcgg ggatctttca tctcatgctc
     1021 cgtaaaaata gttactcgac ccttcgcggc atgatgacgc aaacgggaag acgagtcaag
     1081 tttccgggaa aggtcaccag tcgataggca gttgcgccgg gcgctctctc acgtcgatgg
     1141 ggaacgaggc ggcgcaccgg tcgcgccacg ccgccgcgtg cgcgcccgac gcaaggaccg
     1201 ccgcgaagcc gctccctcgg acgaagcggg tgaaggccgc gagcgacgac cggtctggcg
     1261 ccgtgtcggc ggggaacagg agcgtcgtcg cgcaccgcgg cgtgaagggg gtatccggca
     1321 cggccgagag atagtaccac gcgccgtcga tgaggaggtt catccgccgg tcgcccaccc
     1381 acgccggctc gacggtgaag gggccgaccg acaccacgcc cgacaggcgc aggtactggc
     1441 cgagtccgga cgcctgagcg atgtcgggca gggtggccac gagccctttc cgccgtttgg
     1501 gtatcccctc cagcgggcgg gtgagcgcca ccggcgaccg tgagcggaac ggcgcctcgt
     1561 cgatgtaaag cgaccgccgc gctttcttga gatggtgcac cgtcacgacc tgcatcgggc
     1621 cgctccggag gtatcggggt ggtggcgggg gttaatcggt ctcggcatcg ggggccggct
     1681 gggggccgtt cggtcttttc cccgaccgac ggcggggacg gcggcgacgg cgcggcgcgc
     1741 cttcaggggc gcccgacgct ttcggcgcgg cggcgcgggc cggcgccggg gtatcggcgc
     1801 cctcctcttc
//
LOCUS       DAOQUE010000537         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_85129_length_1810_cov_3.702564, whole genome shotgun sequence.
ACCESSION   DAOQUE010000537 DAOQUE010000000
VERSION     DAOQUE010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85129_length_1810_cov_3.702564"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PKH10_12775"
     CDS             <1..1078
                     /locus_tag="PKH10_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNT29036.1"
                     /translation="VLYDVLHPPKHMQDAIVARFKVMANLDIAEKRVPQDGRIKLKMG
                     KGREMDFRVSVLPCIFGEKVVLRLLDKGNLQVDMTKLGFFEEQLEWFRDAIHRPYGMI
                     LLTGPTGSGKATTLYSALTELNKTDVNISTAEDPVEYNLEGINQVPVREQVGLTFASA
                     LRSFLRQDPDVIMVGEIRDFETAEIAVKAALTGHMVMSTLHTNDAPSAVTRLLNMGIE
                     SFLVTSSLNLIAAQRLGRMVCQACKEEFPIEKHVLKDLGITDRMIDEIAGGVPRIFKG
                     RGCDACSGSGYKGRIAFYEVLVLSEGVKEAVLNGASAMELKQLGIREGMKTMRTSANF
                     RLLEGKVSIDEVLRNTAPDGAESI"
     gene            1111..>1810
                     /locus_tag="PKH10_12780"
     CDS             1111..>1810
                     /locus_tag="PKH10_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNT29037.1"
                     /translation="MVDAASVSLQDLLTLLIEQNASDLHITSGSPPQLRIDGSLRPVN
                     LPPFTPTDTKRLAYSILTERQKKMFEENNELDLSFGVKNLSRFRANIFMQRGAIAAAI
                     RVIPYKILDFSALGLPEVVEEIAKKPKGLVLVTGPTGSGKSTTLASMIDYINRNRKGH
                     IVTIEDPIEFIHTHKNCIVNQREIGSDTASFAKALRVVLRQDPDIVLIGEIRDIETME
                     TALAVSETGHLCFAT"
BASE COUNT          389 a          564 c          513 g          344 t
ORIGIN      
        1 cgtcctgtac gatgtccttc acccgcccaa gcacatgcag gatgccatcg tcgcccgctt
       61 caaggtcatg gcgaaccttg atatcgccga gaagcgtgtc ccgcaggacg gtcgcatcaa
      121 gttgaagatg ggcaaaggac gcgagatgga tttccgcgta tcggtcctcc cctgcatctt
      181 cggcgaaaag gtcgttctcc gtctcttgga caagggcaac ctgcaggtcg acatgaccaa
      241 actgggcttt tttgaggagc agttggagtg gttccgcgat gcgatacatc gtccgtacgg
      301 tatgatactg ctcaccggcc ccaccgggtc gggtaaggcg accacgctct attcggcgct
      361 caccgaactc aacaagaccg atgtcaatat ctcgaccgcc gaggacccgg tcgaatacaa
      421 cctcgagggt atcaatcagg ttccggtccg tgagcaggtc gggctcacct tcgcctcggc
      481 gctccggtcc ttcttgcgtc aggatcccga tgtcatcatg gtcggtgaga tccgtgactt
      541 cgagacggcc gagatcgcgg tcaaggccgc gctcaccggt cacatggtca tgagcacgct
      601 ccacaccaac gacgccccct cggcggtcac ccgtctgctc aacatgggta tcgagagttt
      661 cctcgtcacc tcttcgctca acctcatcgc ggcccagcga ctcggcagaa tggtctgtca
      721 ggcctgcaag gaggagttcc cgatagagaa gcatgtgctc aaggatctcg gcatcaccga
      781 ccgcatgatc gatgagatcg ccggaggcgt tccgcgtatc ttcaaggggc gcgggtgcga
      841 tgcctgttcc ggctccgggt acaagggccg tatcgcgttc tacgaggtac tcgtgctcag
      901 cgagggggtc aaggaggcgg tgttgaacgg cgcgagcgcc atggaactca agcaactcgg
      961 tatccgcgaa ggaatgaaaa cgatgcgcac gagcgccaac ttcagactgc ttgagggcaa
     1021 ggtttccata gacgaggttc tgcgcaacac cgcacccgac ggcgccgaga gcatatgacg
     1081 aaaggcacga tggacaatgc gaggtaattg atggtcgacg cggcaagtgt atccctgcag
     1141 gatctgttga cgctccttat cgaacagaac gcctccgacc ttcacatcac ctccggtagc
     1201 ccgccgcaac tccgcataga cggctccctg cgaccggtga acctgccgcc cttcacgcct
     1261 accgacacca aacggctcgc ctacagcatc ctcaccgagc ggcagaagaa gatgttcgag
     1321 gagaacaacg aactcgacct ttcgttcggt gtcaagaacc tcagtcgttt tcgcgcaaac
     1381 atcttcatgc aacgcggcgc catcgcggcc gccatccgcg tcatacctta caagatactc
     1441 gatttctcgg cgctcggcct gcccgaggtg gtcgaggaga tcgcgaagaa acccaagggg
     1501 cttgtgctcg tcaccgggcc gaccgggtcg gggaaatcga cgacgctcgc ctcgatgata
     1561 gattacatca accgtaaccg caaaggtcac atcgttacaa tagaagaccc gatagagttc
     1621 atccacaccc ataagaactg catcgtcaac cagcgcgaga tcgggtccga caccgcttcg
     1681 ttcgcgaagg cgcttcgcgt cgttctgcgt caggatcccg atatcgtgct catcggcgag
     1741 atccgcgaca tcgagaccat ggagacggcg ctcgccgttt ccgagaccgg gcacctgtgc
     1801 ttcgcgacgc
//
LOCUS       DAOQUE010000538         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_85231_length_1809_cov_3.128848, whole genome shotgun sequence.
ACCESSION   DAOQUE010000538 DAOQUE010000000
VERSION     DAOQUE010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85231_length_1809_cov_3.128848"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PKH10_12785"
     CDS             <1..734
                     /locus_tag="PKH10_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29038.1"
                     /translation="TEAVRIEERAAYLFNLSRAFFALGEIKEAEQIEMRAIAQAERAS
                     FTYPVVVLPAPYAFYREAQALFTPVSPDWRSDPLVRRSAGLLLLLLIAFFWGGIGVPS
                     ISLRHCIECGGIICEECGGTDDEVCITCRVVKAGKKLVSSEEIKTHTRRRERWAAFQR
                     ALAVLLAVPAPGAGLLYNDRVIEGTLYATLFGSVLVLFSLPHFFPFVALTLPVPEVVP
                     LIGYAVLAFFWLLSFYRTWRVAHNE"
     gene            751..1704
                     /locus_tag="PKH10_12790"
     CDS             751..1704
                     /locus_tag="PKH10_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29039.1"
                     /translation="MLAGELKPGAIGDVWEEIAAGEMTGVLEVTSGKDLWQFSFREGA
                     LTVARKRGVDSAAHVVEVLLESGILRQETVRDAVKKQAKTMKSCLEILMEEGQVPLLL
                     YSRVISAVIRLHLLDVMDLHKGHYQFAVKETLREEPGAKPLDMPRFRAFLDRFDRDAK
                     LLKNLRRGLFSPVDVLDDIRFPLPRKTLFYSYASSDFDLIDFFVRMADLHAKGSIAVV
                     GGFFRARPQAIAAAFILRFIIFLFAAWVAAAVFLIPPREQTSATKVAAHATREKAALL
                     QAFSHFETGEEVDVERLMRTGLISTDEYRVIRPGPAQEPLP"
BASE COUNT          317 a          560 c          570 g          362 t
ORIGIN      
        1 acaccgaagc ggtcaggata gaggaacgtg ccgcatacct gttcaacctg agccgcgcct
       61 tctttgcgct cggcgagata aaggaggcgg agcagataga gatgcgcgcc atcgcgcagg
      121 ccgaacgggc cagtttcacc tatccggtgg tcgtgcttcc cgcgccctac gccttttacc
      181 gcgaggcgca ggcacttttc acgccggtct cacccgattg gcggagtgac ccgctggtcc
      241 gccgttcggc cgggctcctg cttctgctgc ttatcgcgtt cttctgggga ggtatcgggg
      301 tcccgagcat ctcgcttcgc cactgtatcg agtgtggcgg catcatctgc gaggaatgcg
      361 gtggcaccga cgacgaggtc tgcatcacct gccgggtggt caaggcgggc aaaaaactag
      421 tgagttccga ggagataaag acccataccc gccgtcgtga acggtgggcg gcgttccagc
      481 gggcactcgc ggtgctcctc gcggtcccgg ccccgggcgc cggccttctg tacaacgacc
      541 gcgtcatcga agggacgctg tacgcgacgc tgttcggttc ggtgctcgtg ctcttctctc
      601 tgccgcattt cttccccttc gtggcgttga ccctgccggt accggaggtg gtgccattga
      661 tcggctatgc ggtgctcgct tttttctggc ttctctcctt ttaccgaacc tggcgggtgg
      721 cccacaatga gtaggacgga ggagcactga atgttggcgg gagaactgaa gcccggcgcg
      781 ataggcgatg tatgggaaga gatcgccgcc ggcgagatga ccggcgtgct cgaggtcacc
      841 tcgggcaagg atctctggca gttctcgttc cgcgaggggg cgcttaccgt tgctcgcaaa
      901 cgaggggtcg acagcgccgc gcatgtggtc gaggtcctgc tcgaaagcgg catcctgcga
      961 caggagacgg tgcgtgacgc ggtcaagaaa caggccaaga cgatgaagag ttgtcttgag
     1021 atactcatgg aggaagggca ggtgcccctg ctcctctatt cgcgggtcat ctcggcggtc
     1081 atccgtctgc atctgctgga tgtcatggac ctgcacaagg ggcactacca gttcgcggtc
     1141 aaggagacac tgcgcgagga gccgggcgcc aagccgctcg atatgccccg gttcagggcc
     1201 tttctcgatc gcttcgaccg cgacgcgaaa ctgctgaaga acctgcggcg cgggctgttt
     1261 tcgccggtcg atgtgctgga tgacatacgg ttccccctgc cgcgcaagac cctcttctac
     1321 agttatgcct cgtccgattt cgacctcata gattttttcg tccggatggc cgacctgcac
     1381 gcgaagggtt cgatcgccgt cgtcggcgga ttcttccggg cccgcccgca ggccatcgcc
     1441 gcggcgttca tcctgcgatt catcatcttc ctcttcgcgg cgtgggtagc ggccgccgtg
     1501 ttcctgatcc cgccgcggga acagacctcg gccaccaagg tcgcggcgca cgcgacacgg
     1561 gagaaagcgg cgctcctgca ggccttttct catttcgaaa cgggtgagga ggtcgatgtc
     1621 gaacggctga tgcgcaccgg actcatatcc accgacgaat accgggtgat ccgccccgga
     1681 cccgctcagg aacctctgcc atgaaaggtg ataccatgcg aagaaccgac cgccttcagg
     1741 tcccgttcga gaatctccgc tactatccgc aactggcgaa atacgcgaag accacggcca
     1801 agaagatcc
//
LOCUS       DAOQUE010000539         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_85896_length_1800_cov_4.878510, whole genome shotgun sequence.
ACCESSION   DAOQUE010000539 DAOQUE010000000
VERSION     DAOQUE010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85896_length_1800_cov_4.878510"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /locus_tag="PKH10_12795"
     CDS             complement(<1..1758)
                     /locus_tag="PKH10_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29040.1"
                     /translation="MSHFGITCSTFEDCDLGQRCFEGRCVQNLKEPKNPMEEQEEFAG
                     LTYATTKMFYNGRVNLPKGFILPYDNLQVLYGKDDLRQSVDPVNGTFWLRLNAVGTSL
                     LVLQTNYPETDRNAPIFLTVFPANGAGYFRRNNIEFSVRETAAALVFLQPALLATVNP
                     FFNAALLERIRNLKTLEYLKQVLEDKMTILSPAVIVSGDVDIQNVVAAAVNELFNDLS
                     TTPLAGIEPLGLVSSDDTTEHFYRVPFKEIPQPDPGEADNVYVKYYNENGPEVKAYNN
                     MPRWVFYFVDAVPLEAALPGEISDPGIDKDAAPVLLVPPRNYVSPKLRYLVQRFVNEE
                     TELLTEAILAENQSNTINAEINAYYNFEPVTEKTPLKYKKKQIEEGFLVGYFPGPDLA
                     DMEPRAFDALWATYFSQLMLPVIQLSGDIGDNFKNVLLNYDQLSDKPISQHPVYLIAR
                     GIRDHGLGLRLNDFMKKREASFESVTYKRDLYEKVTEVFQNAFQGRTMSSVSFIDDIL
                     EQTQSPSFITQLQKASNLVYGQIAPPDIVRRFKGMDAPVIEFTDAVCNHDTGRDTYYF
                     NETEEIIDDDDEPAIIYPTE"
BASE COUNT          347 a          461 c          584 g          408 t
ORIGIN      
        1 ttcggtcgga tagatgatgg ccggttcatc gtcgtcgtcg atgatctctt cggtctcgtt
       61 gaaataatag gtgtcacgac cggtgtcatg gttgcagacg gcgtcggtga actcgatgac
      121 cggcgcatcc atgcctttga aacgacgaac gatatcgggg ggcgctatct gtccgtacac
      181 gagattggac gccttttgca attgcgtgat gaaggagggg ctttgggtct gctcgaggat
      241 gtcgtctatg aaggaaacgc tcgacatggt ccggccctgg aatgcgttct ggaacacctc
      301 ggtcaccttt tcatagagat cccgcttata ggtcaccgac tcgaaggagg cctcgcgttt
      361 cttcatgaaa tcgttgagcc gcaggccgag cccgtgatcg cggatacccc gggctatcag
      421 atagaccgga tgttgcgaga tgggcttgtc ggaaagttgg tcgtagttga gcaggacatt
      481 cttgaagttg tcgccgatgt cgccggacaa ctggatgacc gggagcatca actgactgaa
      541 ataggtcgcc cagagcgcat cgaaggcgcg cggctccatg tcggcgagat cgggaccggg
      601 gaagtagccg acgaggaagc cctcttctat ctgcttcttc ttgtatttga gcggggtctt
      661 ttcggtgacc ggctcgaagt tgtagtaggc gtttatctcg gcgttgatag tgttcgactg
      721 gttctcggcg agtatcgcct cggtgagcag ttccgtttcc tcgtttacga agcgttggac
      781 gagatagcgg agtttgggcg agacatagtt cctcggcggc acaagcagca ccggcgcggc
      841 atccttgtct atgccggggt cgcttatctc tcccggcagg gcggcttcaa gcggcaccgc
      901 gtcgacgaaa tagaacaccc agcgcggcat gttgttgtag gccttcacct cgggaccgtt
      961 ctcgttgtag tacttgacat acacattgtc ggcctcgccc ggatccggtt gagggatctc
     1021 cttgaaaggg acgcggtaga aatgttcggt cgtgtcgtcg gaggagacga ggccgagcgg
     1081 ctcgatgccg gcgagtggtg tcgtagaaag gtcgttgaac agttcgttga ccgccgcggc
     1141 cacgacattc tggatgtcca catcgcccga gacgatgacc gcgggcgaga ggatcgtcat
     1201 cttgtcctcg aggacctgtt tgagatattc gagtgttttg agattgcgta tccgttcgag
     1261 gagcgcggcg ttgaagaagg gattgaccgt cgcgaggagc gccggctgaa ggaatacgag
     1321 cgcggcggcc gtttcgcgga cgctgaattc gatattgttc cggcggaaat agccggctcc
     1381 gttggcgggg aacaccgtga ggaatatggg cgcgttccga tcggtctccg gatagttggt
     1441 ctgcagaacg agcagtgagg taccgaccgc attgagccgc agccagaatg tgccgttgac
     1501 cgggtccacg ctttgacgga ggtcgtcctt gccgtagagg acctgcagat tgtcgtaagg
     1561 caggatgaag cccttcggca gattgacgcg accgttgtag aacatcttgg tcgtggcata
     1621 ggtcagtccc gcgaactcct cctgttcctc catcgggttc ttcggctcct tgaggttctg
     1681 gacgcaccgc ccttcgaaac agcgctgtcc gaggtcacag tcctcgaagg tcgaacaggt
     1741 gatgccgaag tgggacacct tcggctcgca ggcggacgca aggagaagag cccccatcag
//
LOCUS       DAOQUE010000540         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_87133_length_1785_cov_5.152601, whole genome shotgun sequence.
ACCESSION   DAOQUE010000540 DAOQUE010000000
VERSION     DAOQUE010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87133_length_1785_cov_5.152601"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="PKH10_12800"
     CDS             complement(<1..1472)
                     /locus_tag="PKH10_12800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNT29041.1"
                     /translation="MRKNLVLVFLLFFAIVLNGDIFDRLFSTSKNGSISQGEVTLFAL
                     HAMQNRYLNKAKLRPEDLLDKALEAIQEDFAEIVVFYDREKHKVFLQIYNKQHAIDVK
                     RMNDLWDIAVVLRQAYSHFEKEYKPEGDREIGDIEYVAVNGILKKLDPHSYIFTPKEF
                     EEFTSSTEGNFGGLGIVIQANEDGEIFVVSPIDGTPAERAGIVSGDVIVQINDESAIN
                     MSLNKAVERMRGQPGTQVSLYVRRKGAPSVIRFDLERAVIKIQSVVSSMPQKGIGYIK
                     LSGFMENSYDQFVSALGDLRKKGMRALVLDMRGNSGGLLNQAIKISDLFLDRGVIVST
                     VGDDERDVSEASRQSSDILDIPVIVMVNEGSASATEIVAAALKKNGRATVVGRRTFGK
                     GSVQNLFRIPRGGGLKLTIAQYLTPGDISIQTIGVEPDIEYQPSYVDPRKLSLFKTRS
                     NIMGEKDLKEHLISAYLPKEPDTPKLTIHYLKPYKTPDEML"
     gene            complement(1496..>1785)
                     /locus_tag="PKH10_12805"
     CDS             complement(1496..>1785)
                     /locus_tag="PKH10_12805"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT29042.1"
                     /translation="HNFFRGITEDIGQGGLFIATHQFYPIGTEFNLTLAIEKREILLP
                     VKVVWIRESTFSPAGSEPGMGLQFLEMTPEQRSVIEDFIKKREPLFFDADT"
BASE COUNT          339 a          484 c          536 g          426 t
ORIGIN      
        1 agcatctcgt cgggcgtttt gtacggttta aggtaatgta tcgtcagttt gggtgtatcg
       61 ggttccttgg gtaggtaggc gctgatgagg tgctccttga ggtccttctc ccccatgatg
      121 ttcgatcggg tcttgaacaa gctgagtttc cgtgggtcga catagctcgg ttggtattcg
      181 atatcgggct cgacgccgat ggtctggatg gagatgtcgc ccggggtgag gtactgggcg
      241 atggtgagtt tcaggccccc gccgcggggt atacggaaca ggttctggac cgaccccttg
      301 ccgaaggtgc gtcgtccgac caccgtggcg cgcccgttct ttttgagcgc cgcggccacg
      361 atctcggtcg ccgaggccga accttcgttc accatcacga tgaccgggat atccagtata
      421 tcgcttgact gtcgggaggc ttcggacaca tcgcgttcgt cgtcaccgac cgtcgacacg
      481 atgaccccgc ggtcgaggaa caggtcggat atcttgatgg cctgattaag cagtccgccc
      541 gagttgccgc gcatgtcgag caccagcgcg cgcatcccct ttttccgcag atcgccgagt
      601 gccgaaacga actggtcgta actgttctcc atgaagccgg agagttttat gtatccgatg
      661 cctttctggg gcatggagga cacgaccgac tgtatcttga tgaccgcccg ctcgaggtcg
      721 aaccggatga ccgacggagc gcccttgcgc cgcacataga gcgagacctg cgtcccgggc
      781 tgaccgcgca tccgttccac tgccttgttg agcgacatgt ttatcgccga ttcatcattt
      841 atctggacga tgacatcgcc cgaaacgatg ccagcccgtt ccgccggcgt gccgtcgatg
      901 ggggagacca cgaatatctc accgtcctcg ttggcctgta tcactatgcc gagcccgccg
      961 aagttgcctt cggtcgagga ggtgaactcc tcgaactcct tgggggtgaa tatgtaactg
     1021 tgggggtcga gtttcttgag tatgccgttg acggcgacat attcgatgtc gccgatctcg
     1081 cgatcgcctt ccggtttgta ctccttttcg aaatggctgt atgcctgccg gagcacaacg
     1141 gcgatatccc agaggtcatt catccgtttg acatcgatgg cgtgctgctt gttgtatatc
     1201 tgaaggaaga ccttgtgctt ctcgcggtcg tagaagacca cgatctcggc aaaatcctcc
     1261 tgtatcgcct cgagcgcctt gtcgagcagg tcctcggggc gcagtttcgc cttgttcagg
     1321 tagcggttct gcatagcgtg cagggcgaac agggtcacct ctccctgtga gatcgagcca
     1381 tttttcgatg tagagaacag tcgatcgaag atgtcgccgt tgagcacgat agcgaagaaa
     1441 aggagaaaaa cgagcacaag attcttacgc atacggctga actccctagg tggtatcagg
     1501 tgtcggcatc aaagaacagg ggctctcttt ttttaatgaa atcctctatc acgcttctct
     1561 gttcgggcgt catttcgagg aactgaagac ccatgcccgg ttcggagccg gcgggggaaa
     1621 aggtgctctc gcgtatccag acgaccttga ccggcagcag tatctcccgt ttctcgatgg
     1681 cgagtgtgag gttgaactcg gtgccgatag ggtagaactg gtgggtggcg atgaagagcc
     1741 cgccctgtcc gatatcctcg gtgatgccgc gaaagaaatt gtgcg
//
LOCUS       DAOQUE010000541         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_87393_length_1782_cov_3.539085, whole genome shotgun sequence.
ACCESSION   DAOQUE010000541 DAOQUE010000000
VERSION     DAOQUE010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87393_length_1782_cov_3.539085"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PKH10_12810"
     CDS             complement(<1..318)
                     /locus_tag="PKH10_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29043.1"
                     /translation="MRRVMFMALSALLFAAACDDPMKMLPGHQIGDPRIIAMKVTEPE
                     LTLDSRPTARLLIGGEGFDQASDISVLWLVPNDPDTLAQIPPEVQEQLTAPYTADFTF
                     PAGM"
     gene            complement(318..>1782)
                     /locus_tag="PKH10_12815"
     CDS             complement(318..>1782)
                     /locus_tag="PKH10_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29044.1"
                     /translation="KRGFIDLYLMLVQELGIFEAANFVTYPFYYDWSAQWVHKFSNRS
                     KLKVMHIGAIDGTRLLTKDDAESARFSNYFNLDLSFYEILAEWTYKAEEFTSLFSPLF
                     VRNSFDGGMGPRAHLGIVNYQFGLYEKISWRLNETNTIHAGLRLSGGLYDMNADFFAP
                     PKEGEVSYNPFGVELVDDKIRGYFNPSFWVMQTISTGSLTLSPGIASMWDTHNGHLFV
                     DPRFMARYKIDDRWTVKGAIGVYSQLPPPDESHEKFGTPGLKPEHSMHLIGGIEFKVT
                     DTIDLDVQGYYKWFWDLVVRTDPNDPTIYANKGIGYATGAELLLRHKMTDNFFGWLTY
                     SFAISRRRDAIGPGGGPAEWRPFDMDINHNITLVASYKFNKYWQLGGRFNFQTGVPYT
                     DLLNVPTMYDADNDIYIPQYRGPVNPDRMPLRHQLDLRLDKYWLFESWILSTYIDVQN
                     VYYQENAVGWAYNKDYTQRQKVSLLPIMVFFGLKGDF"
BASE COUNT          349 a          477 c          583 g          373 t
ORIGIN      
        1 catgccggcg ggaaaggtga aatcggcggt gtagggcgcc gtgagttgct cctgcacctc
       61 gggcggtatc tgcgcgaggg tgtcgggatc gttcgggacg agccagagca cggagatatc
      121 gctcgcctga tcgaagcctt ccccgccgat gagcagtcgg gccgtcggac gcgagtcgag
      181 cgtcagttcc ggttcggtga ccttcatcgc gataatgcgt gggtcgccga tctggtgacc
      241 cggcaacatc ttcatcgggt cgtcgcacgc ggcggcgaaa agaagcgcag agagcgccat
      301 gaacatcacg cggcgcatca gaaatccccc ttgagaccga agaacaccat gataggcagg
      361 agcgacacct tctggcgttg cgtgtagtct ttgttgtagg cccagccgac ggcgttctct
      421 tgataataga cattctgcac atcgatgtag gtggagagta tccacgactc gaacagccag
      481 tatttgtcga gccgcagatc gagttggtgc cggagcggca tacgatcggg attgaccggg
      541 ccgcgatact gcgggatata gatgtcgttg tcggcgtcgt acatggtcgg gacattgagc
      601 aggtcggtgt aggggacgcc ggtctggaag ttgaatcgcc cgccgagttg ccagtacttg
      661 ttgaacttgt aggacgcgac gagcgtgatg ttgtggttga tgtccatgtc gaaggggcgc
      721 cactccgccg gaccgccgcc cggtccgatc gcgtcccggc gacggcttat cgcgaaactg
      781 taggtgagcc agccgaagaa gttgtcggtc atcttgtggc gcaggagcag ttcggctccc
      841 gtcgcgtagc cgatgccttt gttggcatag atggtcgggt cgttcgggtc ggtgcggacc
      901 acgaggtccc agaaccattt gtagtagccc tgcacatcga gatcgatggt gtcggtcacc
      961 ttgaattcga tcccgccgat gaggtgcatc gaatgttccg gtttcagccc cggagtgccg
     1021 aacttttcat gtgactcgtc ggggggcggc agttgcgaat agacgccgat cgcccccttg
     1081 accgtccagc ggtcgtcgat cttgtagcgc gccatgaacc gggggtcgac gaagagatgg
     1141 ccgttgtggg tgtcccacat cgatgcgatg ccgggcgaaa gcgtcaagga cccggtcgat
     1201 atggtctgca tgacccagaa cgacgggttg aaatacccgc gtatcttgtc gtcgaccagt
     1261 tcaacgccga aaggattgta ggagacctcc ccctccttgg gcggcgcgaa gaaatcggcg
     1321 ttcatgtcgt acagaccgcc cgacaggcgt agaccggcgt ggatagtgtt cgtctcgttc
     1381 agtcgccacg atatcttctc gtagagaccg aactggtagt tcacgatgcc gagatgggcg
     1441 cgcgggccca tgccgccgtc gaaactgttc cggacaaaga gcggcgagaa gagcgaggtg
     1501 aactcctccg ccttgtaggt ccattcggcg agtatctcgt agaaactgag gtcgagattg
     1561 aagtagttgc tgaaccgggc cgattccgcg tcgtccttgg tcagcaatcg agtgccgtct
     1621 atggcgccga tgtgcatgac cttgagtttg ctcctgttcg agaatttgtg cacccactgt
     1681 gccgaccaat cgtagtaaaa ggggtaggtg acgaagttcg ccgcctcgaa gatgcccagt
     1741 tcctgcacga gcatgaggta gagatcgatg aagccgcgct tg
//
LOCUS       DAOQUE010000542         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_87821_length_1777_cov_4.852497, whole genome shotgun sequence.
ACCESSION   DAOQUE010000542 DAOQUE010000000
VERSION     DAOQUE010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87821_length_1777_cov_4.852497"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="PKH10_12820"
     CDS             complement(<1..855)
                     /locus_tag="PKH10_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT29045.1"
                     /translation="MRRMLARMIRKELMQLRRDRRMFPILFLAPIIQLIVLGYAANFD
                     IDHIPVAVCDHDKSEESADFLDHFFGSGYFTRAVNASADRDIDPVMDRGVVQLGLVVP
                     RGFGEAVRGGEATKVQLLVNGSDTNYASNGFNYAAQITARYSSAILVERMAALGVSSV
                     PGVDLRQRVWYNPELLTRHFMVPALLGLILMVITIMLTAMAIVREKESGTIEMLVVTP
                     LRKLALLAGKILPFLAIGLIEAVLVSLVTVYLFGIPFKGQAWLLLLGSLLFLFNTLGV
                     GLLVSTISQ"
     gene            complement(855..>1777)
                     /locus_tag="PKH10_12825"
     CDS             complement(855..>1777)
                     /locus_tag="PKH10_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT29046.1"
                     /translation="GEYFRLKTMFVSRADLDAALDDGSVMVGLVIPEGFTAALERGET
                     APVQGFIDGSDGRKATIVQGYLQAYTTAYGQRVVADWALRMGRKVSLPITHEGRIWYN
                     PELKSSLFLIAGLIVFILMITGTISTALSVVRERERGTMEQLLVSPLSAVTVIIGKTL
                     PYLAISAVSTLIILTVGNVAFDVVIKGSMLLLAVAALLFVLAALGQGILISTITTSQQ
                     VAFFAAALSSILPSLLLSGFIFPISGMPLIIQAITMVVPAKYFVDLLRGIMMRGAGFE
                     TGMYDLLALVLFSIFMITVSAARLKKVRLV"
BASE COUNT          384 a          544 c          557 g          292 t
ORIGIN      
        1 ctgcgatatg gtcgagacca gcagcccgac ccccagcgtg ttgaacagga acagcagcga
       61 gccgagcagg agcagccacg cctgcccctt gaacggaatg ccgaagagat acaccgtgac
      121 gagagagacc agtaccgcct cgatgagccc gatggcgagg aacggcagga tcttcccggc
      181 gagaagcgcc agtttgcgca gaggggtgac aaccagcatc tcgatggtgc ccgattcctt
      241 ctcgcggact atcgccatcg cggtgagcat gatggtgatg accatgagga tgagaccgag
      301 aagcgccggc accatgaagt gacgcgtgag caattcgggg ttgtaccaga cccgctggcg
      361 cagatcgacg ccggggaccg acgatacgcc gagcgccgcc atccgttcga ccagtatcgc
      421 cgacgaataa cgggccgtta tctgcgccgc gtagttgaag ccgttcgagg cgtagttggt
      481 gtcggacccg ttgacgagca actgtacttt ggtcgcctcg ccgccgcgca ctgcttcgcc
      541 gaagccgcgt ggcaccacga gtcccagttg caccacgcca cggtccatca ccgggtcgat
      601 gtcgcggtca gcggaggcat tcacggcgcg ggtgaagtag ccgctcccga agaaatgatc
      661 gaggaaatcg gcgctctcct cgctcttgtc gtggtcgcag accgccaccg ggatgtgatc
      721 gatgtcgaag ttggcggcgt acccgaggac gatgagctgg atgatcggcg caagaaaaag
      781 gatcgggaac atccgccggt cgcgccgcaa ctgcatcagt tccttgcgga tcatccgcgc
      841 gagcatcctg cgcatcacac caacctcacc ttcttgagcc gcgccgccga gaccgttatc
      901 atgaagatag agaaaagcac gagcgcgagc agatcgtaca tccctgtctc gaacccggcg
      961 ccgcgcatca tgatgccgcg caaaagatcg acgaaatact tcgccgggac caccatcgtg
     1021 atcgcctgaa tgatgagcgg catccccgat atcgggaaga tgaatcccga gaggagcaat
     1081 gacggcaata tgctcgaaag cgccgccgcg aagaaggcga cctgttgaga ggtcgttatc
     1141 gtcgagatga ggatcccctg tccgagcgcc gcgaggacga acagcagtgc cgcaacggcg
     1201 agcaaaagca tcgagccctt gatgacgaca tcgaaagcga cgttgccgac cgtcaggatg
     1261 atgagggtgc tcaccgccga gatggcgagg tacggaagcg tcttgccgat gatgacggta
     1321 acggccgaga gcggcgaaac gagcagttgt tccatcgtgc cgcgttcccg ttcgcggacc
     1381 actgacagcg ccgtcgagat ggtccccgtt atcatgagga tgaagacgat gagccccgcg
     1441 atgaggaaga ggctcgactt gagttcggga ttgtaccaga tgcggccttc gtgcgttatc
     1501 gggagcgaca ccttccgccc catccgcaac gcccagtcgg cgaccacccg ctgtccgtag
     1561 gcggtggtgt aggcctgcag atagccctgc acgatagtcg ccttgcgccc gtcggaaccg
     1621 tcgatgaacc cctgcaccgg cgccgtttcg ccccgttcga gcgccgcggt gaagccttcg
     1681 ggtatcacga gccccaccat gaccgagccg tcatcgagcg ccgcatcgag atccgcgcga
     1741 ctgacgaaca tcgtcttgag tctgaagtat tcgccgg
//
LOCUS       DAOQUE010000543         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_88144_length_1773_cov_3.614086, whole genome shotgun sequence.
ACCESSION   DAOQUE010000543 DAOQUE010000000
VERSION     DAOQUE010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88144_length_1773_cov_3.614086"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /gene="argF"
                     /locus_tag="PKH10_12830"
     CDS             <1..746
                     /gene="argF"
                     /locus_tag="PKH10_12830"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNT29047.1"
                     /translation="GKKESIEDSAKVLGRFYDGIEFRGFKQETVEALARYSGVPVWNG
                     LTDIDHPTQVLADLLTVEEHVAKPLNKVKLVYMGDARNNMGNALCIICAKMGMHFVAC
                     APRSLFPEKKLVDEMLKVAKETGAKLEFIDDPAKAVKGADVIYTDVWVSMGEEHLFGE
                     RIRLLKRYQVTMDIMKKTGNPDAIFLHCLPSFHDLETKVGKEVYEKHGVKEMEVTDEV
                     FRSRHSKVFDEAENRLHTIKAVMVATAGR"
     gene            820..>1773
                     /locus_tag="PKH10_12835"
     CDS             820..>1773
                     /locus_tag="PKH10_12835"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNT29048.1"
                     /translation="MDRARALSVFFSIIFFCASLGAVGKEIGLSQLMLDNWTATQGLP
                     QNSVLTMVQTRNGYLWMGTEEGFVRFDGISFTVFDKGNLPLETQHILWMIEDRKLPVL
                     WVGTDGGGLVRFDYRTYATQVFTEKDGLPNNSVVRIVQNADGSLWVATRKGLFLFRDG
                     KVQQILRKKDGLPTDDLKAMVMDDEGRLWLGGRAGRIGWVQDGKPNILDSLDAAVSNL
                     FIDAQGKLFVATQRGLFSLAKGKEIFRPVQAEHLQNYASSVVFEDSTGTLWVGTEEAG
                     LFRIRPDEKRGIMPEDKFYFTNIATVFEDREGSIWVGTRGAG"
BASE COUNT          364 a          500 c          581 g          328 t
ORIGIN      
        1 tgggcaagaa agagtcgata gaggacagcg ccaaggtgct cgggcggttc tacgacggca
       61 tcgagttccg cggcttcaag caggagacgg tcgaggcgct cgccagatac agcggcgtcc
      121 cggtgtggaa cgggctcacc gacatcgatc atccgacgca ggtgctggcc gacctgctga
      181 cggtggagga acatgtcgcc aagccgctga acaaggtgaa actggtctac atgggcgatg
      241 cgcgcaacaa catgggcaac gcactctgca tcatctgcgc caagatgggg atgcatttcg
      301 tcgcctgcgc cccgaggtcg ctgttccccg aaaagaaact cgtggacgag atgctgaagg
      361 tggcgaagga gaccggcgcg aaactggaat tcatcgacga ccccgccaag gcggtcaagg
      421 gggccgatgt catctacacc gatgtgtggg tctcgatggg ggaggaacac ctgttcggtg
      481 aacggatacg cctcctcaag cgctatcagg tcaccatgga catcatgaaa aagaccggca
      541 atcccgacgc gatattcctc cactgcctgc cctccttcca cgacctcgag acgaaggtcg
      601 gcaaggaggt ctacgagaag catggcgtga aggagatgga ggtgaccgac gaggtgttcc
      661 gctcccgtca ctccaaggtg ttcgacgagg ccgagaaccg gcttcacacc atcaaggcgg
      721 tcatggtcgc caccgcggga cgataagccg cccgttttct tgttttttga tattctttcc
      781 gctagtatgg tgcccgaaca agggaatcgg cgggatacta tggaccgagc gcgcgcctta
      841 tcggtattct tttcgatcat cttcttttgc gcctctctcg gggcggtcgg caaggagatc
      901 gggctttcgc aactgatgct cgataactgg accgccacgc aggggttgcc gcagaactcg
      961 gtcctgacga tggtccagac ccgcaacggg tacctctgga tggggaccga ggaggggttc
     1021 gtccggttcg acggcatttc cttcaccgtg ttcgacaaag gcaatctgcc gcttgagacc
     1081 cagcacatcc tctggatgat agaggatcgg aaactgccgg tcctctgggt ggggaccgat
     1141 ggtggcgggt tggtccggtt cgattaccgc acctacgcga cgcaggtctt cacggagaag
     1201 gacggcctgc cgaacaactc ggtggtccgc atcgtgcaga acgccgacgg ttcgctgtgg
     1261 gtggcgaccc gcaaggggct tttcctcttc agggacggca aggtccagca gatcctgcgt
     1321 aaaaaggacg gtctgccgac cgacgatctc aaggcgatgg tgatggacga tgaggggcgg
     1381 ctctggctcg gcgggcgcgc cggtcgcatc ggctgggtgc aggacggcaa accgaacatc
     1441 ctcgattcac tcgacgccgc ggtgtccaac ctgttcatcg acgcgcaggg aaaacttttt
     1501 gtggcgaccc agagagggct cttctcgctc gcgaagggca aggagatatt ccgtcccgtt
     1561 caggcggaac acctgcagaa ttatgcctcg agcgtcgtgt tcgaggacag tacgggaacg
     1621 ctttgggtcg ggaccgaaga ggcggggctg ttccgcatcc gccccgacga gaagcggggc
     1681 atcatgccgg aggacaagtt ctacttcacc aacatagcca cggtgttcga ggaccgcgag
     1741 ggctcgatct gggtcgggac gcgcggcgcg ggg
//
LOCUS       DAOQUE010000544         1772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_88210_length_1772_cov_3.591147, whole genome shotgun sequence.
ACCESSION   DAOQUE010000544 DAOQUE010000000
VERSION     DAOQUE010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88210_length_1772_cov_3.591147"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="PKH10_12840"
     CDS             <1..1668
                     /locus_tag="PKH10_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29049.1"
                     /translation="VRLDNGDFVAMVDSVTRPIPEKRYKTITVMLLPVVGCGRYLVSG
                     DPVPPDTGDGFDEEWLWQGASKEDPLWRNERVETPANPEPSTAEKATINQTYASFHEL
                     PTVQPYTDREEFKKMVHYMVTDPTDRAFLRENPDTVRVIQRPVNDWTLAEAEMRRSAF
                     AYTLAEKADFFSDLVQEKMINELGMMSHWNEAAQRYDQDGDSMLWTGVYVATMAWKYL
                     IDQTQGSLDRMIKSLDGTLQCVEIVPDVTTFARTLRLHLDDGDPEFHEGTGPFAGIDW
                     KEGGNNDMFKGFLIGHYWAWFVLKDSADTRAAGLITRMKNNLKRLHDNCEIAKDLRMN
                     QLATSLLLYAMNKGTLSETIYKNESATIWSYVEEYIDQGGLPTNEYGISDWSGNHLGV
                     WEFFVLREVYESVGDGTRVGDMQAALYEKGDAMRPARQGLFQLIVGARGVPQMMSDID
                     SGIECLQEVPMERGLYAVDRLWSPDFCMSPIPELFWKNDWTSPTADRTQSLRAYPHFE
                     RGASSYYWKDNIYKGLRGSQGTGGDTGLDFLAGYWFARRYGLITEND"
BASE COUNT          387 a          543 c          549 g          293 t
ORIGIN      
        1 gtgcggcttg ataacggcga cttcgtcgcg atggtcgaca gcgtcacaag accaatccct
       61 gaaaagcgct acaagacgat aacggtcatg ctcctgccgg tggtcggctg cggtcgatat
      121 ctggtgtcgg gtgatccggt gccgcccgat accggcgacg ggttcgacga ggaatggctc
      181 tggcaggggg catcgaagga agatccgctc tggcgcaacg aacgggtcga gacccccgct
      241 aaccccgaac cgtcgaccgc cgaaaaggcg acgatcaacc agacctacgc gtcgttccac
      301 gaactcccga cggtccagcc ctacaccgac cgagaagagt tcaagaagat ggtccactac
      361 atggtgaccg atccgaccga ccgcgccttt ctgcgcgaga accccgacac ggtgcgggtc
      421 atccagcggc cggtgaacga ctggacgctc gccgaggccg aaatgcgccg cagcgctttc
      481 gcctacacgc tcgccgagaa agctgacttc ttctcagacc tcgtgcagga gaagatgata
      541 aacgaactcg gcatgatgtc gcactggaac gaggcggccc agcgctacga tcaggacggc
      601 gacagcatgc tctggaccgg cgtctatgtc gcgacgatgg cgtggaaata cctcatcgac
      661 cagacacagg ggtcgctcga ccggatgatc aagtcgctcg acgggacgct tcagtgcgtc
      721 gagatagtcc ccgatgtgac caccttcgcg cggaccctca ggttacacct cgacgacggc
      781 gatcccgaat tccacgaagg gaccggcccg ttcgccggta tcgactggaa agagggcggc
      841 aacaacgaca tgttcaaagg attcctcatc ggccactact gggcgtggtt cgtcctcaaa
      901 gacagcgccg acacgcgtgc cgcggggctc atcacccgga tgaagaacaa cctcaaacgg
      961 ctccacgaca actgcgagat cgccaaagac ctgcggatga accaactcgc cacctcgctc
     1021 ctgctctacg cgatgaacaa gggaacgctc tccgagacga tctacaagaa cgagagcgcg
     1081 accatctgga gttatgtcga agaatacata gatcagggcg gtctgccgac caacgaatac
     1141 ggcatcagcg actggtcggg caaccatctg ggcgtctggg aattcttcgt cctgcgcgag
     1201 gtctatgagt cggtgggcga cggcacccgc gtgggtgaca tgcaggcggc gctctacgaa
     1261 aagggcgatg cgatgcgacc ggcgcggcaa gggctgttcc aactgatcgt cggggcgcgc
     1321 ggcgtgccac agatgatgag tgacatagat tcggggatcg agtgtctgca ggaggtgccg
     1381 atggagcgcg gactctatgc ggtcgaccgg ctgtggagcc cagatttctg catgtcgccg
     1441 ataccggaac tcttctggaa gaacgactgg acctcgccga ccgccgaccg gacgcagagt
     1501 ctgcgcgcct atcctcattt cgaacgcggg gctagcagtt actactggaa ggacaatatc
     1561 tataaaggtc tgcgcgggag tcaagggacc ggcggcgata ccgggctcga tttcctcgcg
     1621 ggctactggt tcgcccgccg gtacggcctc atcaccgaga atgactgata cgctactcat
     1681 ctttcgcctc acggggcaca accgcaccgc catgcggtcg ctcatcgcgt cgctcgaagc
     1741 ggcgggtacg ccgccgggag cggtcaaagc gg
//
LOCUS       DAOQUE010000545         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_88374_length_1770_cov_3.510787, whole genome shotgun sequence.
ACCESSION   DAOQUE010000545 DAOQUE010000000
VERSION     DAOQUE010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88374_length_1770_cov_3.510787"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="PKH10_12845"
     CDS             complement(<1..1394)
                     /locus_tag="PKH10_12845"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNT29050.1"
                     /translation="MRATQLFVQTLKEVPKEAQIASHILLYRAGMIQKLASGIYNYLP
                     LALRSLRKIENILREELNKRGCQEVEMPVVQPAELWQESGRWGYYGPELLRFKDRKGG
                     DFCLGPTHEEVITDMARRNLRSYKQLPWNLYQIQTKFRDEIRPRFGLMRGREFTMKDG
                     YSFDVDEEAALKTYRLMYEAYTAIFTRCGLKFKAVDAATGAIGRDKSHEFQVLADSGE
                     DMILSCSTCDYAANEEKARTKLVETKVDPAQFKAPETVLTPDKRSVEEVAAFLKLPVE
                     RFIKSMIYLVDEEPVLVMVRGDREVNEAKIQAHLQANKVELAPDAVIVEVTGAAVGFA
                     GPVGMKKKIKMVADHTVTGILNAATGACKSDHHVVNINMGRDFTPDIVGDFIKTKKGE
                     PCPKCDGVLEEFRGIEVGQVFFLGTKYSEKMHCVFIDAAGAEKPAVMGCYDISVDRTM
                     SAAIEQNHDEHGII"
     gene            1530..1664
                     /locus_tag="PKH10_12850"
     CDS             1530..1664
                     /locus_tag="PKH10_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29051.1"
                     /translation="MRLCLLVILTAFLCGACNKKGELFPDYAIQNAAESDGDLNEPAR
                     "
BASE COUNT          296 a          569 c          512 g          393 t
ORIGIN      
        1 cagatgatgc cgtgctcgtc gtgattctgt tctatcgccg ccgacatcgt acggtcgacg
       61 ctgatatcgt aacagcccat gaccgccggt ttctcggccc ccgccgcgtc gatgaagacg
      121 cagtgcatct tctcgctgta cttcgtcccg aggaagaaga cctgccccac ctcgatgccg
      181 cggaattcct caagtacgcc gtcgcacttc gggcagggct cgcccttctt cgtcttgatg
      241 aagtcgccga cgatatcggg ggtgaagtcg cgccccatgt tgatattcac gacatgatga
      301 tcgctcttgc aggcaccggt cgcggcgttg aggatgccgg tcaccgtgtg gtcggcgacc
      361 atctttattt ttttcttcat gccgaccggt cccgcaaagc cgaccgccgc gccggtgacc
      421 tcgacgatca ccgcgtcggg agccagttcg accttgttgg cctggaggtg cgcctgtatc
      481 ttcgcctcgt tgacctcgcg gtcgccccgc accatcacga gcaccggctc ctcatcgacg
      541 agatagatca tcgacttgat gaaccgctcg accggcagtt tcaggaaggc cgcgacctct
      601 tccaccgacc tcttgtcggg ggtgaggacc gtctccggcg ccttgaactg ggccgggtcg
      661 accttcgttt cgaccagttt cgtgcgcgcc ttctcctcgt tggcggcgta gtcgcacgtg
      721 gagcaggaaa gtatcatgtc ctcgcccgaa tcggcgagca cttggaactc gtggctcttg
      781 tcgcggccga tggcccccgt cgccgcgtcg accgccttga acttgagccc gcaccgcgtg
      841 aatatcgccg tgtaggcctc gtacatgagc cggtaggtct tgagcgccgc ttcctcatcg
      901 acatcgaacg aatagccgtc cttcatcgtg aattcgcgac cgcgcatgag tccgaaacgg
      961 ggccgtatct cgtcgcggaa cttggtctgt atctggtaga ggttccacgg caactgtttg
     1021 tacgaccgga ggttgcgccg cgccatatcg gtgatgacct cctcgtgggt cggcccgaga
     1081 cagaagtcgc cccccttgcg gtccttgaag cggagcagtt cgggaccgta gtaaccccag
     1141 cggccgctct cctgccacaa ctctgccggt tgcaccaccg gcatctcgac ctcctgacag
     1201 ccgcgcttat tgagttcttc gcgcaggatg ttctcgatct tgcggagact ccgtagggcg
     1261 agcgggagat agttgtagat gccgctggcc agtttctgga tcatcccggc gcggtacagg
     1321 aggatgtggc tcgcgatctg cgcctccttg ggcacctctt tcagcgtctg tacgaacagt
     1381 tgcgtcgctc tcatcgtgtg caccctttcg gtaaaaggac aaaaacagcg gttactgtac
     1441 cgcgaaacgg cgaaaaagca atgttcgaat aacttgcttc ggcccgcgcc ctcttctata
     1501 ctgcctccgg tgtgcgaccg gagtacgcga tgcggctttg tcttctcgtc attctgacgg
     1561 cgttcctgtg cggcgcctgc aacaaaaaag gggagctctt ccccgactac gcgatacaga
     1621 acgccgccga gagcgacggc gacctgaacg aaccggcgcg atgaaccaca cacggcgcgg
     1681 ctactttttt ctttttctcg gggtcttcct gtggggcatc cacggcccgg cgggccattt
     1741 cctcgccctg cgcggcgtcg acatgctctg
//
LOCUS       DAOQUE010000546         1769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_88447_length_1769_cov_5.387398, whole genome shotgun sequence.
ACCESSION   DAOQUE010000546 DAOQUE010000000
VERSION     DAOQUE010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88447_length_1769_cov_5.387398"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PKH10_12855"
     CDS             complement(<1..790)
                     /locus_tag="PKH10_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29052.1"
                     /translation="MNRIVWLSAVLIALFLVACESNEPGGIRLHYSWVDSEGNDLTPP
                     DLTQLFAWGELKYGAETKTTEAVWLGADDATLRFTGLPYGVSMSLTLTMRAADTEGDD
                     SDIVGLDDVPEVRTDNIVYYCTSQSFSLTRGKVTDVHNACVMQEGPAAGEGNKPGAPV
                     MTLSYTDDDDKETTIDAATEFKTPKKIIAISFTAPNDNFDTVVVANDTGFALGRKEHP
                     KPEKVNGAYRIESHDLDDGLTITGDGSRTIALKLRNALGFESLAA"
     gene            complement(973..>1769)
                     /locus_tag="PKH10_12860"
     CDS             complement(973..>1769)
                     /locus_tag="PKH10_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNT29053.1"
                     /translation="SIRTDPEMVYDSVCEKVTLFGGSSGAYYDDTWEWDGVNWAQMDP
                     ATDPAGRSAHAMAYDSSRAKAVLFGGFTGSVFSDETWEWGENRWEQKNTGNKPPVLMN
                     HAVAYDAARGKTILFGGASEVNTYNETWLWDGGAHDRSGQIMTVSRESSGITDDGYIQ
                     SLSVLFHAGGLGDITGTPTNGVDLLTWKHDSWVTVASNEADPDHLEPVTWATTDNTEI
                     QTLLNSGLRKFNFAVVPTAPNGFLTDMGTIATDYAEVIVRYTISEE"
BASE COUNT          309 a          529 c          518 g          413 t
ORIGIN      
        1 tcgcggcgag cgattcaaag ccgagggcgt tgcggagttt cagcgcgatg gtgcggctgc
       61 cgtcgcccgt tatcgtcagg ccgtcgtcga ggtcgtggct ttcaatgcgg taggcgccgt
      121 tcaccttctc cggtttgggg tgttccttcc tgcccagcgc gaagccggtg tcgttggcga
      181 cgaccacggt gtcgaagttg tcgttcggag cggtgaacga tatcgcgatg atctttttcg
      241 gggtcttgaa ttcggtggcg gcatcgatgg tggtctcttt atcatcgtcg tcggtgtagg
      301 aaagggtcat caccggggcg cccggtttgt tcccttcgcc ggccgccggt ccttcctgca
      361 tgacgcaggc gttgtggaca tcggtcactt tcccgcgggt cagggagaag gactgcgagg
      421 tgcagtaata gacgatattg tcggtgcgca cctcggggac atcgtcaagt ccgacgatgt
      481 cggaatcgtc gccttcggtg tcggcggcac gcatcgtgag cgtcagggac atcgagacgc
      541 cgtaggggag cccggtgaag cggagcgtcg cgtcatcggc gccgagccag accgcctcgg
      601 tggtcttggt ctcggccccg tatttgagtt cgccccatgc gaacagttgc gtcaggtcgg
      661 gcggtgtgag gtcgttcccc tcgctgtcca cccacgagta gtgtagccgt atgccgcccg
      721 gctcgttgct ctcgcaggcg acaaggaaca gcgcgatcaa tacagccgac aaccacacga
      781 tacggttcat ggtctcacct catttcaacg gttcgatcgg gcgaataaga ttcgccccta
      841 caaccgggca cccacaagga gtgcccctac aattcggttt ttctgttcac cgttcactgc
      901 tcaccgctca ctgctcaccg ctcactgctt accttctcac tcctctactt ctcacgcctc
      961 acgcctcact cctcactcct cactgatcgt atacctcacg atcacctcgg cgtagtcggt
     1021 ggcgatggtg cccatgtcgg tcagaaagcc gttgggggcg gtcggaacga cggcaaagtt
     1081 gaatttgcgc aggccgctgt taaggagcgt ctgtatctcg gtgttgtcgg tcgtggccca
     1141 ggtgaccggc tcaaggtggt cgggatcggc ctcatttgac gcaacggtga cccaactgtc
     1201 gtgcttccat gtcagaagat caacgccatt ggtcggcgtg ccggtgatat cgccgagtcc
     1261 gccggcgtgg aatagcaccg aaagagattg gatatagccg tcgtcagtga taccggaact
     1321 ttcccgcgaa acagtcatga tctggccgga acggtcgtgc gctccgccat cccaaagcca
     1381 tgtttcattg taggtgttta cttcacttgc ccctccgaag aggatggtct tccctcgtgc
     1441 ggcatcataa gcgaccgcat gattcatcaa caccggaggt ttgttaccgg tgtttttctg
     1501 ttcccatcgg ttctcccccc attcccatgt ttcatcgctg aatacggatc ccgtaaaccc
     1561 gccgaacagg accgctttgg cgcgagaact gtcgtaggcc atcgcatgag cggatcgtcc
     1621 cgcgggatcg gtcgccggat ccatctgtgc ccagttcacg ccgtcccatt cccaagtatc
     1681 gtcgtagtat gctccgctag aacctccgaa aagggtcact ttttcgcaaa cgctatcgta
     1741 caccatctcg ggatcggttc gtatcgagg
//
LOCUS       DAOQUE010000547         1769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_88458_length_1769_cov_3.967328, whole genome shotgun sequence.
ACCESSION   DAOQUE010000547 DAOQUE010000000
VERSION     DAOQUE010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88458_length_1769_cov_3.967328"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="PKH10_12865"
     CDS             <1..1058
                     /locus_tag="PKH10_12865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT29054.1"
                     /translation="FIDRFFSRRQWMLAMQALLMLVASALTLLSPFPGLVPVMFVIFF
                     VGSVFAATNDVATDGYYMEALSEERQARWVGLRVLAYRIAMMAGTGVVATVGAVASWP
                     LAFLLAAVIMGAFFLYHLFFLPLPPVRPPVAATATRTPYFEAFLTYLRRPRIVAILLF
                     IVFLRAGEYMLHAMVSPFFVSIGIAAHYGWISSFVGLPATIAGALAGGWLIARFGLRR
                     MLWPLVLFQNLTNLLYTGLAYRLQGAEYGELSRVIAAATVNGVENLSSGMGNAVLVVF
                     LMRLCRDDFRAAHYAIGSGLMGLAGIFAGSVSGFIVRDHGYALMFLASFLAAAPALLS
                     IPRLPIPTEFDKRPADT"
     gene            1092..>1769
                     /locus_tag="PKH10_12870"
     CDS             1092..>1769
                     /locus_tag="PKH10_12870"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNT29055.1"
                     /translation="MQITPAQKRRVAIFFIGGGALFVIVIGIIIGDQLLKREDCYYSN
                     FRDVSVAGLSEGSSVKFQGMNIGSVRDIAIDEMDSAIIRIHFCLKPDTPMKEGTTAQL
                     GSVGITGLKFLELKGGGKWRDIPTGGEVPSVSSQWDQITGKAEVIAEKLEIILNNVNN
                     IVQEVKPETVKQIANNVAGISASLNTILAQNKDNVRTLTGESAIFMRKLNDSMDELDK
                     VVTDVRAM"
BASE COUNT          290 a          588 c          534 g          357 t
ORIGIN      
        1 ccttcatcga ccggttcttc tctcggcggc agtggatgct cgcgatgcag gcgcttctga
       61 tgctcgtcgc gtcggcgctg acgctcctgt cgccgttccc ggggctcgtg ccggtgatgt
      121 tcgtcatatt cttcgtcggg tcggtgttcg ccgctaccaa cgatgtggcc accgacggct
      181 actacatgga ggcgctgtcc gaagagcggc aggcgcggtg ggtcggcctg cgcgtgctcg
      241 cctaccgcat cgccatgatg gccggcaccg gcgttgtcgc gacggtcggc gcggtcgcca
      301 gttggccgct cgcgttcctg cttgccgccg tcatcatggg ggcttttttc ctctatcacc
      361 tgttcttcct cccgctcccg ccggtcaggc cgcccgtcgc ggcgaccgcg acgcgcacgc
      421 cctatttcga ggcgttcctc acctatctcc ggcgtccccg catcgtcgcg atactgctgt
      481 tcatcgtctt tttgcgggcc ggtgaataca tgctccatgc gatggtgagc ccgttcttcg
      541 tgtcgatagg catcgcggcc cattacggct ggatatcgtc gttcgtcggc ctgcccgcga
      601 ccatcgccgg cgcgctcgcc ggcggctggc tcatcgcgcg gttcggcctg cggcgcatgc
      661 tgtggccgct cgtgctgttc cagaacctca ccaacctgct gtacaccgga ctcgcgtatc
      721 gcctgcaggg cgccgagtac ggagaactat cccgcgtgat agccgccgcg acggtgaacg
      781 gcgtcgagaa cctctcgtcg gggatgggca acgcggtgct cgtcgtgttc ctcatgcggc
      841 tgtgccgcga cgatttccgc gccgcccact acgccatcgg gtcggggctc atgggactcg
      901 ccggcatctt cgccgggtcg gtgtcgggct tcatcgtccg cgatcacggc tacgcgctga
      961 tgttcctcgc cagtttcctc gccgccgccc cggcgcttct ctccatcccg cgacttccca
     1021 taccgacaga atttgacaaa cgccccgcgg atacctaaaa taccccgtac tatttttctc
     1081 ctgtaggtgg aatgcagatc accccggctc agaaacgacg ggtcgccatc ttcttcatcg
     1141 gcggcggcgc cctgttcgtc atcgtcatcg gcatcatcat cggcgaccag ttgctcaagc
     1201 gggaggactg ctactactcc aatttccgcg atgtctcggt cgccgggctc tcggaggggt
     1261 cgagcgtcaa gttccagggg atgaacatcg gcagcgtccg cgacatcgcc atcgacgaga
     1321 tggattccgc catcatccgg atacatttct gcctcaagcc cgataccccg atgaaggagg
     1381 ggacgaccgc ccaactgggg agcgtcggca tcaccgggct caagttcctc gaactcaagg
     1441 gcggcggcaa gtggcgcgac ataccgacgg gcggcgaggt gccctccgtc agttcgcagt
     1501 gggaccagat aaccggcaag gccgaggtca tcgccgagaa actggagatc atcctgaaca
     1561 atgtgaacaa catcgtgcag gaggtgaagc ccgagacggt caagcagata gcgaacaatg
     1621 tcgccggcat atcggcctcg ctcaacacca tactggcaca gaacaaggac aatgtccgca
     1681 cgctgaccgg cgagtcggcc atattcatgc ggaaactcaa cgacagcatg gatgaactcg
     1741 acaaggtggt gaccgatgtc cgcgcgatg
//
LOCUS       DAOQUE010000548         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_88801_length_1765_cov_3.218129, whole genome shotgun sequence.
ACCESSION   DAOQUE010000548 DAOQUE010000000
VERSION     DAOQUE010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88801_length_1765_cov_3.218129"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="PKH10_12875"
     CDS             complement(<1..1631)
                     /locus_tag="PKH10_12875"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNT29056.1"
                     /translation="MVGRHVAISFFFAFLFLLVACTSEEPEEMITCVTAFDCPVDWFC
                     EDNVCVEFPDSQPPTDKDTSGTVPDADADSGTDIPLDQDPVTDRDADADIDADADIDA
                     DADKDAPVDDGGVDDGVVGDDSDELLSDEPVGDDGPVSDDAVVTDDTPVTDFDNVVPP
                     QSCGDGKNTVADGLVLYLRFDEPTLFTTAYDSSASLLHGKGTAGVSLGVAGVRGNAMS
                     FNQSSNFQIADTATLDMTGPMTVMGWVYLDPHANNIWPYPVVIKGNGRQANYYVWREA
                     DRKLNFGFHRWEETGTGCGAKSTIDIDDSTWHYFAGTYDGTYVRLYVYGTDGVLQDQI
                     TSDPCPQEPLPNDRAFTVGQIPPGTEYPMHYYDSTGYIDELRIYTRALSPGEIGSVMS
                     SHTEYCDDGDDTVVGDGCTDCQVDTGYICYGTPSTCEVEPSWTCTHSVNICDSTYDYY
                     DSSMGDYYYRSWVGTSLEAISGGAVKWTSPAFSTYQWTDYSAGAKCWGTWYNYTFDKG
                     QTVDLNYSTKGAYNPNQCYFQLKDGASGAGNVITTT"
BASE COUNT          353 a          541 c          518 g          353 t
ORIGIN      
        1 gtggtggtga tgacattgcc ggcgccgctc gccccgtcct tcaactggaa gtagcactga
       61 ttcggattgt aggcgccttt ggtggaataa ttaagatcca ccgtctgccc tttgtcgaag
      121 gtgtagttgt accaagtgcc ccagcacttc gcgccagcag aataatcggt ccattgatag
      181 gtcgagaagg cgggcgaggt ccacttcacc gccccgcccg agatggcctc cagggaggtg
      241 cccacccaac tgcgatagta gtaatccccc atagaactat cgtaataatc gtaggtgctg
      301 tcgcagatat tcaccgaatg ggtacaggtc cacgagggct cgacctcgca ggtcgaaggg
      361 gtaccgtaac agatgtatcc ggtatcgacc tgacagtcag tacaaccatc cccgacgacg
      421 gtatcgtcgc cgtcatcgca gtattcggtg tgcgaactca tgacggaccc tatctcaccc
      481 ggcgaaaggg cgcgggtata tatccgtagt tcgtcgatgt agccggtcga gtcgtagtag
      541 tgcatcggat attcggtccc gggcggtatc tggccgacgg tgaaggcacg gtcgttcggc
      601 aacggttcct ggggacaggg gtcggaggtg atctgatcct gcaggacacc gtcggtgccg
      661 tagacataga gccgcacata ggtgccgtcg taggtcccgg cgaaataatg ccaggtgctg
      721 tcgtcgatgt ctatggtcga cttggcgccg caaccggtgc ctgtctcttc ccaccggtgg
      781 aacccgaagt tgagtttccg gtccgcctcg cgccacacat agtagttcgc ctgcctgccg
      841 ttccccttga tcacgacggg gtaaggccag atgttgttcg cgtgcgggtc gagatagacc
      901 caccccatga cggtcatggg acccgtcatg tcgagcgtcg cggtatcggc tatctggaag
      961 ttgctactct gattgaacga catggcattg ccccggacac cggcaacacc cagagagacg
     1021 ccggccgttc ctttgccgtg gagcagggat gccgacgagt cgtacgccgt cgtgaagagc
     1081 gtcggctcgt caaaacgcag gtagagcacc agcccgtccg ccacggtgtt cttaccgtcg
     1141 ccacacgact gcggaggcac cacattgtcg aagtcggtca ccggcgtatc gtcggtcaca
     1201 acggcgtcat cggacacagg accatcatcc cccaccggtt cgtcgctcaa cagttcgtcg
     1261 ctgtcatcgc cgaccacccc gtcgtccacg cccccgtcgt cgaccggcgc atccttgtcg
     1321 gcatcggcat cgatgtccgc atccgcgtcg atatcggcat cggcgtcccg atcggtcacc
     1381 ggatcctgat cgagcgggat atcggtgccg ctatcagcgt ccgcgtcggg caccgtacct
     1441 gaggtatcct tatccgtcgg gggttgactg tcggggaact cgacacagac attatcctcg
     1501 cagaaccaat ccacgggaca gtcgaacgcg gtgacgcaag tgatcatctc ctcgggctct
     1561 tcggaggtgc aggcgacaag cagaaaaaga aaggcgaaga agaacgatat cgcgacatgt
     1621 cgaccgacca tgtatgacct ccgcttcata gacatcatag cgtgatgata ataagcccgt
     1681 cgccggacct gacgccgctg ttcatcgtcg tgccggtatt acccggagcg gccacatatc
     1741 ccgagccgcc gccgccgctc ccgcc
//
LOCUS       DAOQUE010000549         1761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_89092_length_1761_cov_5.121336, whole genome shotgun sequence.
ACCESSION   DAOQUE010000549 DAOQUE010000000
VERSION     DAOQUE010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89092_length_1761_cov_5.121336"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..977)
                     /gene="gpmA"
                     /locus_tag="PKH10_12880"
     CDS             complement(231..977)
                     /gene="gpmA"
                     /locus_tag="PKH10_12880"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNT29057.1"
                     /translation="MYKVVLLRHGESVWNKENRFTGWTDVDLSEKGVQEAHAAGQLLK
                     KEGYAFDIAYTSVLKRAIRTLWITLDEMDLMWLPVIRNWRLNERHYGALQGLNKAEMA
                     QKFGEEQVHIWRRSYDVRPPALTPEDERFPGRDRRYAALTKEELPLTECLDDTVKRFL
                     PLWEKEIVPHIRNGKRIIIAAHGNSLRSLVKHLDNVPNDEIVALNIPTGIPLVYELDR
                     GMKPLKHYYLGDEAAVKAAMESVKNQGKAK"
     gene            complement(1131..>1761)
                     /locus_tag="PKH10_12885"
     CDS             complement(1131..>1761)
                     /locus_tag="PKH10_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29058.1"
                     /translation="GMTVGALVAQSPELQAQYDEQGWMDLPVYASVTIEEKTLSIIKR
                     VRVTKGVPAHFNPEIIRIKAVYKENGALRTVFADRDGTIELPQDAIPEYLGLDPQMAD
                     AATHGNDLLVYRWQFAEDPDTATDLRFCDADCPSQEYLAVDRVTPATKEIMVDMGNIS
                     KKLKDSPHDTDIVLNFYLVVRDKADDSTNLEDTRFGLDFTTMKVVLKRP"
BASE COUNT          301 a          534 c          543 g          383 t
ORIGIN      
        1 gaggcggcac ggcgcatcgg ctgcgactgc tgtctctgtt ctcgcggcca ccagtcgaag
       61 gagcggcttc tcgccgccgg ggtcccggtc gtggattcgt tcggagaact cactatttga
      121 cacccccgca agccccctgc atcagggggc cttcgatagt ggctccctgt cgcagggagc
      181 tgtccgcagg actgagggtg ttatgagatc ggacgcgatg aatcgcgccc ctacttggcc
      241 ttgccctgat tcttgaccga ctccatagcc gccttcaccg ccgcctcgtc gccgaggtag
      301 taatgtttga gcggtttcat gcccctgtcg agttcataca ccagcgggat gccggtcggg
      361 atgttgagcg cgactatctc atcgttgggg acattgtcga gatgcttgac gagcgaccgc
      421 agactgttgc cgtgcgccgc gatgatgatg cgcttgccgt tccgtatgtg gggcactatc
      481 tctttttccc acagcggcag gaaccgtttc acggtgtcgt cgaggcactc ggtgagcggc
      541 agctcctctt tggtgagcgc ggcatagcga cggtcgcggc cggggaaccg ctcgtcctcg
      601 ggggtgagcg ccggcggccg gacatcgtaa gagcgccgcc agatgtggac ctgttcctcg
      661 ccgaacttct gggccatctc ggccttgttg agtccctgca gcgcgccgta gtgccgctcg
      721 ttcagccgcc agttgcggat gaccggcagc cacatgagat ccatctcatc gagcgttatc
      781 cagagcgtcc gtatagcccg cttgagcacc gaagtatagg cgatgtcgaa ggcgtagccc
      841 tccttcttca gcagttgccc cgccgcgtgg gcctcctgca cgcccttctc cgatagatcg
      901 acatcggtcc agccggtgaa acggttctct ttgttccaga cgctctcgcc gtggcgcaac
      961 aacactactt tgtacatcgg atcctccgca agtgaacaat gatgcgaacg gtccgatgat
     1021 acccgaaaac gggccgaaca caaaaaattc gggcaggttc ctgccgctcg cggagcgggg
     1081 gacctgacca ccggaaaatt ttgcgaagag aaggggaggc gacggtagcg tcagggtctt
     1141 ttcagcacca ccttcatcgt cgtgaaatct agaccgaagc gggtgtcttc gaggttcgtc
     1201 gaatcgtccg ccttgtcgcg caccactagg tagaaattga ggacgatgtc cgtgtcgtgt
     1261 ggagaatcct ttagtttttt cgatatgttg cccatgtcca ccatgatctc cttggttgcc
     1321 ggggtgacac ggtcgacagc gagatattcc tgactcgggc agtcggcgtc gcagaagcgc
     1381 aggtcggtcg ccgtgtcggg gtcctcggcg aactgccagc ggtagacgag caggtcgttg
     1441 ccgtgtgtcg ccgcgtcggc catctgcggg tcgagcccca gatattccgg tatcgcatcc
     1501 tgcggcagtt cgatcgtacc gtcgcgatcg gcgaacaccg ttctcaacgc gccgttctcc
     1561 ttgtagaccg ccttgatgcg aattatctcg gggttgaaat gggcggggac ccccttggtg
     1621 acacggaccc gtttgatgat ggagagcgtc ttctcctcga tggtgaccga cgcgtagacc
     1681 ggcaggtcca tccagccctg ctcatcgtac tgcgcctgca gttccggact ttgcgccacg
     1741 agggcgccga ccgtcatgcc g
//
LOCUS       DAOQUE010000550         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_90533_length_1744_cov_4.361753, whole genome shotgun sequence.
ACCESSION   DAOQUE010000550 DAOQUE010000000
VERSION     DAOQUE010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90533_length_1744_cov_4.361753"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="PKH10_12890"
     CDS             complement(<1..1111)
                     /locus_tag="PKH10_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29059.1"
                     /translation="MRPTPLFFFLLLITACAPDLPELPADIEYSAGTDYRATANDERP
                     DDDIICPAGAHPHVGRCVVVDLVVATTAGYEAPRSGRVLIAPDERLEELRELEFPMPL
                     GGADGTDLTIATAPDRLMVVGRDGADTLWVYEPRNDTPRFTKILSPVAGYTNFHDVLW
                     DGARYIVSANELDRLLLFDETGSPAGEIPLATFAFDGVAPAPSALLRNGERAYAALQL
                     IGDDWLSRGGRIARFERDGTPLTPMVLPLANPTSKLAVEPLLSPDRLFVSCSGSYQAR
                     DGGIVRTDLFTGETTVILRETTDDLSPLNVKVGDLAVTPEGDIYFTAFDEDWRGHLQV
                     LRRDGAVHRISDDINAFAAIPLEASPFSGRVYFFDQ"
     gene            complement(1108..>1744)
                     /locus_tag="PKH10_12895"
     CDS             complement(1108..>1744)
                     /locus_tag="PKH10_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNT29060.1"
                     /translation="FLLPEETLVFDGGVEQRFWDRRVSIEVNGFYQETRDLIDFSYGC
                     DGMLCGRYRNLGWLFARGIEAIAVIRPDERFTISANYTFADTDSWETVLYDGRKWRDT
                     RPVLRRPAHIANLWADWTPDNAFNANISLTLIGPRHDIEYRYPYEPAVVRLPAALLGA
                     LAITVRPGPLVDIFARVENLFDERYELVRGYGTPGFSLYGGMRLHFGEALP"
BASE COUNT          330 a          507 c          607 g          300 t
ORIGIN      
        1 gctgatcgaa gaaatatacc ctgccgctga aggggctggc ttcgagcggt atcgccgcga
       61 acgcattgat gtcgtcggag atacggtgca ccgccccatc acgccggagc acctgtaggt
      121 gtccccgcca atcttcgtcg aacgcggtga agtagatatc gccctccggc gtcaccgcaa
      181 ggtcgccgac cttgacattg agcggggaga ggtcgtcggt cgtttcacgc agtatgacgg
      241 tcgtctcgcc ggtgaacaga tctgtacgga cgatgccgcc gtcgcgcgcc tgatacgagc
      301 cggaacagga aacgaacagc cgatcgggcg aaaggagcgg ctcgaccgcc agtttcgatg
      361 tcgggttcgc gagcggcagg accatcggcg tgagcggtgt cccgtcccgt tcgaagcggg
      421 cgatgcgtcc tccgcgcgaa agccagtcat caccgatcag ttgcagggcg gcgtaggcgc
      481 gttccccgtt acgcaaaagc gccgaggggg cgggggcgac gccatcgaaa gcgaaagtgg
      541 cgagcggtat ctcgcccgcc ggcgaaccag tttcgtcgaa cagaagcagg cgatcgagtt
      601 cgttggccga tacgatgtat cgtgcgccgt cccagaggac atcgtggaag ttggtgtacc
      661 cggcgaccgg agagaggatc ttcgtgaagc ggggcgtgtc gttacgcggc tcgtagaccc
      721 acagcgtgtc ggcgccgtcg cgaccgacga ccatcagccg gtcgggagcg gtcgctatcg
      781 tgagatccgt gccgtcggcg ccgccgagcg gcatcgggaa ttcaagttcg cgcaactctt
      841 ccagccgttc gtccggtgcg atgagtacgc gacccgatcg cggcgcttcg tacccggcgg
      901 tcgtcgcaac gacgagatcg acgacgacgc agcgcccgac atgggggtgt gccccggcgg
      961 gacagatgat gtcgtcatcg ggcctttcgt cgttcgctgt cgcccggtaa tcggtgccgg
     1021 cgctgtattc tatgtcggcg ggcagttcgg gcagatcggg ggcgcaggcg gtgatgagaa
     1081 gcaagaagaa aaagagtggc gtcggtctca tggcagggcc tccccgaaat gaagtctcat
     1141 gccgccatat aacgaaaacc ccggtgtgcc gtagccccgg acgagttcgt agcgttcgtc
     1201 gaaaaggttc tccactcgcg cgaagatgtc tacgagcggg ccgggtcgca ccgtgattgc
     1261 gagcgccccg agcagggcgg ccggcaagcg tacgacggca ggttcgtagg gataacggta
     1321 ttcgatatcg tgacgcggtc cgatgagggt gagcgagatg ttggcgttga aggcgttgtc
     1381 gggcgtccag tcggcccaga gattcgcgat gtgcgccggg cggcgcagga ccggacgggt
     1441 atcgcgccac tttcgaccgt cgtagagcac ggtctcccac gagtcggtgt cggcgaaggt
     1501 gtagttggcc gagatggtga atcgttcgtc ggggcggatg accgcgatcg cctcgatgcc
     1561 gcgcgcgaag agccagccga gattgcggta acgcccgcag agcatcccgt cgcacccgta
     1621 ggaaaaatcg atgagatcgc gcgtttcctg ataaaacccg ttcacctcga tggaaacgcg
     1681 gcggtcccag aaccgctgtt cgacgccgcc gtcgaagacg agcgtctctt cagggagtag
     1741 gaac
//
LOCUS       DAOQUE010000551         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_90855_length_1740_cov_3.858160, whole genome shotgun sequence.
ACCESSION   DAOQUE010000551 DAOQUE010000000
VERSION     DAOQUE010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90855_length_1740_cov_3.858160"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PKH10_12900"
     CDS             <1..332
                     /locus_tag="PKH10_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HNT29061.1"
                     /translation="KQSNYTLRCYSGEKVSGHCPSVDVLFQSVAACAGGNALGIILTG
                     MGSDGAKGLLEMHLAGALTLAQRPEGCPVYSMPRAAVDIGAVRAQLSIEGIAAYINRS
                     FEEGSLW"
     gene            326..814
                     /locus_tag="PKH10_12905"
     CDS             326..814
                     /locus_tag="PKH10_12905"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT29062.1"
                     /translation="MVNVERTYVIDNDLDAISGVVSAIMDQLRTAFSKTQPFEVALYE
                     AIHNAIEHGNLAIPFVEKKALIESGRYDQTIASRRRLAPYVTRTVRIRLVITDDAVTF
                     FVADEGDGFDWRAWLPDPDTTEAATGVSGRGITIMRSVFDTVSYNDRGTEVILTKKRG
                     RF"
     gene            814..>1740
                     /locus_tag="PKH10_12910"
     CDS             814..>1740
                     /locus_tag="PKH10_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT29063.1"
                     /translation="MQAQGKENEKRRVLIVDADEQVTAVLALKLRQQGYETLSLSSTV
                     NALAMIRDFLPHLIISEMVLPELSGVDFLKRIKMNPETAHIPFIFLSSSRNVEDKIIA
                     HEMGAESFFSKPIFIKVLINRINDFFQERDFREILSSSHEQDLFSGNLQNISVIDLLN
                     IVHENRRQGIISLVSGGGEKGIIHFADGTIVRIEREGADDKNGEEIFYSLLPWSEGEF
                     TVTYAAHTAEPNITEPFERLMVSAMSWLSEYQAEIGEMPPLDVQLHLRFSPFVEHLAK
                     LPDTIGALLSLVPEQGIRVGELIEKGGDDRKRT"
BASE COUNT          376 a          555 c          490 g          319 t
ORIGIN      
        1 aaaagcaaag caactatact ctgcgctgct acagcggcga aaaagtcagc ggacattgcc
       61 cctccgtgga tgtgttgttc caatcggtgg cggcgtgcgc gggcggtaac gcgttgggca
      121 ttatcctgac cggcatggga agcgacggcg ccaaggggct tctcgagatg cacctcgccg
      181 gagcgctcac gctcgcccag cgtcccgagg gctgtccggt ctacagcatg ccgcgcgccg
      241 cggtcgatat cggcgcggtc cgcgcgcaac tctcgataga gggcatcgcc gcctacatca
      301 accgctcttt cgaggagggt tcgctgtggt gaatgtcgag cggacctatg tgatcgacaa
      361 cgacctcgac gcgataagcg gcgtcgtctc ggccataatg gatcagttgc ggacggcctt
      421 ttcgaagacc cagccgttcg aggtggcgct gtacgaggcg atacacaacg cgatagagca
      481 cggcaacctc gccatcccgt tcgtcgaaaa gaaggcgctc atcgagagcg gtcgctacga
      541 ccagacgatc gcctcccggc ggcgactcgc accctacgtc acccgcaccg tccgcatcag
      601 gttggtcatc accgacgatg cggtcacctt tttcgtcgcc gacgagggcg acggcttcga
      661 ctggcgggcg tggcttcccg accccgacac gacggaggcc gcgaccggcg tgagcgggcg
      721 cggcatcacg ataatgcgaa gtgtgttcga cacggtatcc tacaacgacc gggggaccga
      781 ggtcatcttg accaagaaaa gggggcgctt ctgatgcagg cacaagggaa agagaacgaa
      841 aaaagacggg ttctcatcgt cgacgccgac gagcaggtga ccgccgtact cgcactgaaa
      901 cttcgccaac aggggtacga aacgctgtcg ctctccagca ccgtcaacgc gctcgccatg
      961 atacgcgatt tcctgccgca cctcatcatc tccgaaatgg tgctgcccga actttcgggc
     1021 gtcgatttcc tcaaacggat caagatgaac cccgagaccg cccacatccc cttcatcttt
     1081 ctttcgagtt cccgcaatgt ggaggacaag atcatcgccc acgagatggg cgcggagtcc
     1141 ttcttttcca aaccgatctt catcaaggtt ctgatcaacc gcatcaacga cttctttcag
     1201 gaacgcgact tccgcgagat actttcctcg tcccacgaac aagacctctt ttccgggaac
     1261 ctgcagaaca tatcggtcat agacctgctc aacatagtcc atgagaaccg gcgacagggt
     1321 atcatctcgc tcgtttccgg cggcggcgag aaagggatca tccacttcgc cgacggcacc
     1381 atcgtccgca tcgagcggga aggcgcggac gacaagaacg gcgaggagat attttattcg
     1441 cttctgccgt ggtcggaagg cgaattcact gtcacctatg ccgcccacac ggccgaaccc
     1501 aacatcaccg aacccttcga acgcctcatg gtatccgcca tgagttggct ctcggaatat
     1561 caggccgaga tcggcgagat gccgccgctc gatgtccaac tgcaccttcg gttctcaccc
     1621 ttcgtcgaac atctcgcgaa actccccgac accatcggcg cccttctttc tctcgtcccg
     1681 gaacagggga tccgggtcgg cgaactcatc gaaaagggag gcgacgaccg gaaacggacc
//
LOCUS       DAOQUE010000552         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_90861_length_1740_cov_3.600000, whole genome shotgun sequence.
ACCESSION   DAOQUE010000552 DAOQUE010000000
VERSION     DAOQUE010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90861_length_1740_cov_3.600000"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PKH10_12915"
     CDS             <1..704
                     /locus_tag="PKH10_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNT29064.1"
                     /translation="SDDLSKVMHQVAGRPIISWILDAVLPFGTAQVVVGHLREQVESF
                     VRPRYPGVRFSVQATQDGTGGAVRCAFAEADPVADRFLILPGDAPLLKKETLERLIAT
                     FDAGRPALAFLTCLLDDPAQYGRVRRSSDGGVTGIVEYRDATEEERALKEVNSGIYLV
                     SRSLLAAFLPRMRNDNRKGEYYLTDIIAFAVSNGHAVTALAEDDAASLSGINSPEELA
                     QADTALRERLTRSKP"
     gene            complement(701..1468)
                     /locus_tag="PKH10_12920"
     CDS             complement(701..1468)
                     /locus_tag="PKH10_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29065.1"
                     /translation="MYGLATSIGGGILVAVIIGLLTGLSGDGGLSYGWWGFGVFIGLF
                     LAIVAFFFISRRVTNKLQALFLRANAEIQKQKADRAIELLKEGYRWNRWAFLVKAQID
                     SQIGVILYSLKKFGEAYKYLQNSNPRLYLAYCLLVIGHLKNNRRKEALDAMELLMRFN
                     KKEAFVFSLCAYLYEEELDDHEQAIAVVKRGLKTLPKNQLLNEHLVALQNRNAFKMER
                     YGEVWYQMFLDRKAVSRLQQKMIKDQQRRMKVSGVVR"
     gene            complement(1541..>1740)
                     /gene="prfC"
                     /locus_tag="PKH10_12925"
     CDS             complement(1541..>1740)
                     /gene="prfC"
                     /locus_tag="PKH10_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNT29066.1"
                     /translation="KPCWLTAKDQKKITEFCKFRSNAVATDKDGNTVFFSESDHMLRH
                     HIAENPDIEFHTTSEFKRATG"
BASE COUNT          307 a          522 c          539 g          372 t
ORIGIN      
        1 gttcggacga cctctccaag gtcatgcatc aggtcgcggg acgccccatc atctcgtgga
       61 tactggatgc ggtcctgccg ttcggcaccg cacaggtggt cgttggtcac ctgcgcgaac
      121 aggtcgagtc gttcgtccga ccgcgctatc ccggggtccg cttctcggtg caggccacgc
      181 aggacggcac cggcggcgcg gtgcggtgcg cctttgccga ggccgatccg gtggcggacc
      241 ggttcctgat attgcccggc gatgctccgc tgctgaaaaa ggagacgctg gagcggctta
      301 tcgccacctt tgatgctggc cggcccgcgt tggcgttcct tacctgcctg ctggatgacc
      361 ccgcccagta cggccgcgtc cgacggtcgt ccgacggcgg cgtgacgggc atcgttgaat
      421 accgcgacgc caccgaggag gagcgtgcac tgaaggaggt gaacagcggt atctatctgg
      481 tgtcgcggtc cctgctcgcc gcctttctgc cgcgcatgag gaacgacaat cggaagggcg
      541 aatactatct caccgatatc atcgcgttcg ccgtgtcgaa cgggcacgcc gtcacggcgc
      601 ttgccgagga tgatgcggca tcgctttccg gcatcaattc acccgaggaa ctcgcgcagg
      661 ccgacactgc cctgcgggaa cggctcacgc ggtcgaaacc ctagcggacg accccgctca
      721 ccttcatgcg gcgctgttgg tcctttatca ttttctgctg aaggcgggag accgccttgc
      781 ggtcgaggaa catctggtac cagacctcgc cgtaccgctc catcttgaac gcgttccggt
      841 tctggagcgc tacgagatgc tcgttgagca gttggttctt ggggagcgtc ttgagcccgc
      901 gcttgaccac cgctatcgcc tgctcgtggt catccagttc ctcttcgtag aggtaggcgc
      961 acagcgagaa cacgaacgcc tccttcttgt tgaagcgcat caggagttcc atggcgtcca
     1021 gcgcctcttt gcgccggttg ttcttgagat ggccgatgac gagcaggcag taggcgaggt
     1081 agagccgggg attcgagttc tgcaggtatt tgtacgcctc gccgaatttc ttgagcgaat
     1141 agaggatgac gccgatctgg ctgtctatct gcgccttgac gaggaacgcc caacggttcc
     1201 agcggtagcc ctccttgagc aattctatcg cgcggtccgc cttctgtttc tgtatctcgg
     1261 cgttggcgcg caggaagagc gcttgcaact tgttggtcac cctgcgcgat atgaagaaga
     1321 acgcgacgat ggcgaggaac agcccgatga agacgccgaa gccccaccac ccgtacgaga
     1381 gcccgccgtc gcccgacagg ccggtcagga gccctatgat aacggcgacg agtatgccgc
     1441 cgccgatgct tgtcgcgaga ccgtacatga gacctccatt agaaagaacg gaccactgta
     1501 gtttctccgg gtcatttttg caataaaaat ccccccggac ctaccccgtg gcccgtttga
     1561 attccgaggt ggtgtggaac tctatgtcgg gattctcggc gatatggtgc cggagcatat
     1621 ggtccgattc ggagaaaaag acggtgttgc cgtccttgtc ggtggcgacg gcgttgctcc
     1681 ggaacttgca gaattcagtt atttttttct ggtctttcgc agtgagccag catggcttgg
//
LOCUS       DAOQUE010000553         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_91170_length_1736_cov_4.177870, whole genome shotgun sequence.
ACCESSION   DAOQUE010000553 DAOQUE010000000
VERSION     DAOQUE010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91170_length_1736_cov_4.177870"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PKH10_12930"
     CDS             <1..566
                     /locus_tag="PKH10_12930"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT29067.1"
                     /translation="AIAEAFAPIVLWIDEIEKALFQEEQSAEIRRVLGWLMTWMQEKS
                     RPVFLVATANNVEILPPELLRKGRFDEIFFIDLPGADERTDILRIHLTRRGRDASRYD
                     IAKLAEAAEQFSGAELEQVVVDALIADYAKGRELAQKTLEEVLRRTVPLAVTYEEQIK
                     KLRMWSRGRARNASGNQRIEALFNTAP"
     gene            591..>1736
                     /locus_tag="PKH10_12935"
     CDS             591..>1736
                     /locus_tag="PKH10_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29068.1"
                     /translation="MAELLTDRAAGSIVNTVYHSFGGTLIKLYGADAGIEGLFFSSRE
                     RVAFPVFDLRPFRRDPLTNLEESLRKYLDGRRLLRASVDPVRGRVLIISFSDVVLTIP
                     LFAGKAVHLTGPDGAMLWSEHRDHDIPPLAEPLRDLLPRVASPGEWQDRFLAGKREAE
                     EDARRRAIEKKKSEIGEKIAKMRAERERALERSASFRATATGIKGELYTLSPHARTAK
                     ITLGDRDIPLDPQKSVLENMNDLFSEAKRLLRGTITLDGRIAEAERALATLNGDAPTT
                     ATVPERKKREPKAKPGAHVPYHRYLAPSGRALLVGRSAADNDELTFKIASPHDLWFHA
                     KDHAGSHVILRKGKGGTATEEEVFLGCCLALFYSRVRETMEGEVWFTE"
BASE COUNT          362 a          562 c          529 g          283 t
ORIGIN      
        1 tcgccatcgc cgaggcgttc gcccccatcg tcctgtggat agacgagata gagaaggcgc
       61 tctttcagga ggaacagtcg gccgagatac gccgcgtgct cggctggctc atgacctgga
      121 tgcaggaaaa gagccgcccg gtgttcctcg tcgcgaccgc caacaatgtc gagatcctgc
      181 cacccgaact cctacgcaag gggcgattcg acgagatatt cttcatcgat ctgcccggcg
      241 ccgacgaacg gaccgatata ctccggatac atctgacccg ccgcggacgg gacgcttccc
      301 ggtacgacat cgccaagttg gcggaggccg ccgagcagtt ctcgggcgct gaactcgagc
      361 aggtggtggt cgacgcgctg atagccgact acgcgaaagg tcgcgaactg gcccaaaaga
      421 ccctcgaaga ggtcctgcgc cgcaccgtac cgctcgcagt gacctacgaa gaacagataa
      481 agaaactccg catgtggtcg cgcggcaggg cccgtaacgc ctccgggaac cagcgcatcg
      541 aggcgctttt caacaccgcg ccgtaaatgt atatctccga actcgccccc gtggccgaac
      601 ttctgaccga ccgggccgcc ggctccatcg tcaacaccgt gtaccattcc ttcggtggca
      661 cactcatcaa actatacggc gccgacgcgg ggattgaggg gctctttttc tcgtcgaggg
      721 aacgggttgc gttcccggta tttgacctgc ggccgttccg tcgcgatccc ctgaccaatc
      781 ttgaggagtc actacgtaaa tatctcgatg gtcgccggct tctgcgtgca tcggtcgacc
      841 ccgtccgcgg tcgggtgttg atcatctcct tcagcgatgt cgtgctcacg ataccgcttt
      901 tcgccggcaa ggcggtacat ctgaccggcc ccgacggcgc gatgctctgg agcgagcacc
      961 gcgaccacga tatccccccg ctcgccgaac cgctccgcga cctgttaccg cgtgtcgcat
     1021 cgcccggtga atggcaggac cggttccttg ccgggaagcg ggaggccgaa gaggatgctc
     1081 gccgccgcgc catcgagaag aaaaagagtg agataggcga gaagatagcg aagatgcggg
     1141 cagaacggga acgggctctc gaacggtcgg catcattccg cgcgacggcg acggggatca
     1201 agggcgagct ctacaccctg tcaccgcacg cacggaccgc gaaaataacg ctgggcgacc
     1261 gcgacatacc gctcgacccc caaaaaagcg tcctcgagaa catgaacgac ctgttcagcg
     1321 aagcgaaacg gctcctgcgc ggcaccatca cgctcgacgg gcggatcgcc gaggcggaac
     1381 gcgcccttgc gaccctcaat ggcgatgcgc ccacaaccgc aacggtaccg gaaaggaaaa
     1441 agcgtgaacc gaaggcgaag ccgggggccc atgtccccta tcaccgctac ctcgcgccat
     1501 cgggacgggc gttgctcgtc ggacgttcgg ccgccgacaa cgacgaactg accttcaaga
     1561 tcgcctctcc gcacgatctc tggttccatg cgaaggacca cgccgggagc catgtcatcc
     1621 tgcgaaaagg caagggaggg accgcgaccg aggaggaggt cttcctcggc tgttgcctcg
     1681 ccctctttta cagcagggta cgcgagacga tggagggaga ggtctggttc acggaa
//
LOCUS       DAOQUE010000554         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_91689_length_1730_cov_4.404179, whole genome shotgun sequence.
ACCESSION   DAOQUE010000554 DAOQUE010000000
VERSION     DAOQUE010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91689_length_1730_cov_4.404179"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1455)
                     /locus_tag="PKH10_12940"
     CDS             complement(145..1455)
                     /locus_tag="PKH10_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29069.1"
                     /translation="MRKSLCILAAAVFLFLVVSCDSKSTAKTDNQPVNDETAGGDEVV
                     TDDTAADNTVPTDDTTPVNCGNGVTDTGEACDGDAKECAQIDNAYIGGWATCKTDCSG
                     YDVSSCTLDPDADIPAGDDTTDNDGVIETDDTTISDDDAPLPSCAGAPYTVIKGEHAL
                     YDGGCQYAITVGSDPVDLYIPNASSSDTLYTAIFMQGAKVDKGFYSNFAKILSSYGIV
                     VAIPNHDSFSGTNMTENKAFNAVWDFVKAATTDGASPLFQRVNIAKVAVMGHSNGGMA
                     AVGIIGNTCSQPTCSGSYQAPLELAAGILYGTNTVMPIIGTVSAFNTRNIPTIFLQGR
                     IDGKAKYDDTLKTLDKTTGTPVVFVALDGMNHYGICDMNNPAGAAADGNVPTTDQALG
                     VETNARWAGMYLKAHLFDDADAHDYVYDGTGDTADTIAAVEQIK"
     gene            complement(1575..>1730)
                     /locus_tag="PKH10_12945"
     CDS             complement(1575..>1730)
                     /locus_tag="PKH10_12945"
                     /inference="COORDINATES: protein motif:HMM:NF014621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNT29070.1"
                     /translation="PDGASTLFDLFAKKLSSLGVTVATGSFGAHMQVALVNDGPVTFI
                     LDSRKRL"
BASE COUNT          333 a          477 c          528 g          392 t
ORIGIN      
        1 cgtcaccgcc ggcggcctcg tcgttcgccg gttggttatc gatctttgcc acatttttgc
       61 tgtcacaggc ggacaggacc gcgataagga acaacagggg tataccgaag agtatcgcct
      121 tcatggctcg cctccgtaga gagatcattt tatctgttcc actgcggcta tcgtatcggc
      181 ggtgtcgccg gtgccgtcgt acacatagtc gtgggcatcg gcgtcgtcga acagatgcgc
      241 tttcagatac atcccggccc aacgcgcgtt ggtctcgaca ccgagcgcct gatcggtcgt
      301 cggcacattg ccgtcggcgg ccgcgcccgc cggattgttc atgtcgcaga tgccgtagtg
      361 attcatcccg tccagtgcga cgaacacgac cggggtgccg gtggtcttgt cgagcgtttt
      421 aagcgtgtca tcgtatttcg ctttgccgtc tatgcgcccc tgcagaaaga tggtgggtat
      481 gttgcgagtg ttgaatgccg aaacagtgcc gatgatcggc atgacggtgt tggtgccgta
      541 cagaatgccg gcggccagtt cgagcggcgc ttgataggag ccggagcagg tcggttgaga
      601 gcaggtgtta ccgatgatgc ccactgcggc catgccgccg ttgctatggc ccatgacggc
      661 gaccttagct atattcaccc gctgaaagag aggcgacgcg ccatcggtcg tcgcggcctt
      721 gacgaaatcc cacacggcgt tgaacgcctt gttctccgtc atgttcgtgc cggagaaact
      781 gtcatgattc ggtatcgcta cgacgatccc gtatgaggaa agtatctttg caaagttgct
      841 gtaaaaacct ttgtcgacct tggctccttg cataaagatg gcagtgtaga gagtgtcgct
      901 tgacgacgcg ttcgggatat agagatcgac cggatcacta ccgaccgtta tcgcatactg
      961 acagccgccg tcgtaaaggg catgttcccc tttgataact gtgtagggtg cgccggcgca
     1021 ggagggaaga ggagcgtcat cgtcggagat cgtcgtatcg tccgtttcga taacgccatc
     1081 gttgtcggtc gtatcgtcgc ccgccgggat gtcggcgtcg gggtcaaggg tacacgacga
     1141 gacatcgtag ccggagcagt cggtcttgca ggtcgcccag ccgccgatgt aggcgttgtc
     1201 tatctgcgcg cactccttgg cgtcgccgtc gcaggcttcg ccggtgtcgg tcacaccgtt
     1261 gccgcaattc acgggcgtgg tgtcatcggt cgggacggtg ttgtcggccg cggtgtcgtc
     1321 ggtcacgacc tcgtcgccgc cggcggtctc gtcgttcacc ggctggttat cggtcttcgc
     1381 ggtgcttttg ctgtcgcatg agacgacaag aaaaaggaac accgccgccg ccaagataca
     1441 aagactcttc cgcatggtca ctcctcctgc taaaaaagaa aaaaacacgg tatctagacc
     1501 tacgctaccg gcaaaaagtt tcctgtcaag ccgttttcct ttccttaaat atatagtgcg
     1561 cgggagcgat gcctttatag acgcttccgg ctgtcgagta tgaaagtgac cggtccatcg
     1621 ttcacgagcg ccacctgcat atgggcaccg aaggagccgg tcgcgaccgt tacgccgaga
     1681 gaggagagtt ttttggcaaa aagatcgaag agcgtggagg cgccatcggg
//
LOCUS       DAOQUE010000555         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_91697_length_1730_cov_3.665672, whole genome shotgun sequence.
ACCESSION   DAOQUE010000555 DAOQUE010000000
VERSION     DAOQUE010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91697_length_1730_cov_3.665672"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKH10_12950"
     CDS             complement(<1..573)
                     /locus_tag="PKH10_12950"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT29071.1"
                     /translation="MSGASTNKNNFYTADRKFYLSQIDLTDIVRDLENGYTDSGPTAL
                     RSYEEAQRWYDMALRNVGDICANFIWPRAEAVDEKGASFKDGVVEWAPETKENMKVLG
                     AAGYMGGTLPRKYGGLNMPVTVNVAIVEMVSQGDASLMNLFGLQDIAVTVYRFGSEEQ
                     KDRVLPRFCSGEVSGSMALTEPEAGSDLQAV"
     gene            complement(573..>1730)
                     /locus_tag="PKH10_12955"
     CDS             complement(573..>1730)
                     /locus_tag="PKH10_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNT29072.1"
                     /translation="SAMEELTATNAQVHDNVTQIHSLSAEVSTNMVSSEKSTEGLSEA
                     TELVQELVSRFKIGRGTFDYNVERVRKFMRARITGKAAALMKPFHGEVVGIFVGDRLP
                     EDTDELFKYGMHRVVFYNNAKLKHLHSIAIKQVVAQFVKREKPDIVLFGATHTGRDIA
                     PLVAAQIHTGLTADCTQLVIDDYRDKGKILYQIRPAFGGNILATIVTPDHRPMMATVR
                     EGVMPLPEHKTMNPVKFEEFTPDFRDEWVRHEFLNIIPKRSKVDLGAANVIVSGGAGV
                     GNKESFKLIFQLAEALGAEVGASRAAVDFGFIEKDHQVGQTGSVVRPKLYIACGISGS
                     IQHRAGMEESKKILAINKDPDAPIFGIAHMGVVGDLREIIPLMIRYLREEG"
BASE COUNT          297 a          549 c          516 g          368 t
ORIGIN      
        1 gaccgcctgc aggtcgctcc ccgcctccgg ctcggtcagc gccatcgaac cggacacttc
       61 gcccgaacag aatctgggca ggacacggtc cttctgctcc tcgctgccga atctatagac
      121 ggtgacggcg atgtcctgca gaccgaagag attcatcagg ctcgcgtcgc cctgcgacac
      181 catctcgacg atagcgacat tgacggtgac cggcatgttg agcccgccgt attttcgcgg
      241 cagggtgccc cccatgtagc cggcggcgcc gagcaccttc atgttctcct tggtctcggg
      301 ggcccattcg accacgccgt ccttgaacga ggcgcctttc tcgtcgaccg cctcggcgcg
      361 cggccagatg aagttggcgc agatgtcacc gacattgcga agcgccatgt cgtaccaccg
      421 ttgcgcctcc tcgtaggaac gcagtgcggt ggggccgctg tccgtatagc cgttctcaag
      481 gtccctgacg atatcggtga gatctatctg cgaaagatag aacttccggt ccgcggtata
      541 gaagttgttc ttgttcgtgc ttgctccaga cattagcctt cctccctcag gtatctgatc
      601 atgagcggga ttatctcccg caggtcaccg acgaccccca tgtgggcgat gccgaatatc
      661 ggcgcgtcgg gatccttgtt gatggccagt atcttcttcg attcctccat gccggcgcgg
      721 tgctgtatcg aaccgctgat gccgcaggcg atgtagagtt tcgggcggac caccgacccg
      781 gtctggccga cctgatggtc cttctcgatg aagccgaaat cgaccgccgc acggcttgcg
      841 ccgacctcgg cgcccaacgc ctcggcgagt tggaagatga gtttgaaact ctccttgttg
      901 cccactccgg caccgcccga aacgatgaca ttcgcggcgc cgagatcgac cttcgaccgc
      961 ttcgggatga tgttcaggaa ctcatggcgc acccattcgt cgcggaagtc gggcgtgaac
     1021 tcctcgaact tcaccgggtt catcgtcttg tgctccggca acggcatgac cccctcgcgg
     1081 accgtcgcca tcatcgggcg atggtcgggg gtcacgatgg tggccagtat gttgccgccg
     1141 aacgccgggc ggatctggta gagtatcttg cccttgtcac ggtagtcgtc tatcaccagt
     1201 tgggtgcagt cggcggtgag tccggtatgt atctgcgcgg ccacgagcgg cgcgatgtcg
     1261 cggccggtgt gggtggcgcc gaagaggacg atgtcgggtt tctcgcgctt cacgaactgc
     1321 gcgacgacct gttttatcgc tatcgaatgg agatgcttca gtttcgcgtt gttgtagaag
     1381 accacgcggt gcatgccgta cttgaacaac tcgtcggtgt cctcgggaag ccgatcgcca
     1441 acgaagatgc cgaccacctc gccgtggaac ggtttcatga gcgccgccgc cttgccggtg
     1501 atgcgcgcgc gcatgaactt gcgcacgcgc tcgacgttgt aatcgaaggt gccacgcccg
     1561 atcttgaacc gggaaaccag ttcctgcacc aactcggtcg cctcggacaa gccctcggtg
     1621 gacttctccg aactgaccat gttggtcgac acctcggccg acagcgaatg gatctgcgtc
     1681 acgttatcgt gcacctgcgc attggtcgcc gtcagctcct ccatcgccga
//
LOCUS       DAOQUE010000556         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_91884_length_1728_cov_4.507472, whole genome shotgun sequence.
ACCESSION   DAOQUE010000556 DAOQUE010000000
VERSION     DAOQUE010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91884_length_1728_cov_4.507472"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKH10_12960"
     CDS             <1..500
                     /locus_tag="PKH10_12960"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNT29073.1"
                     /translation="NRPTVAIVGYTNAGKSTLFNAIVGEKVVRGSDEFFSSIDPKIRL
                     VTLFGRKFFLLDTVGFIEGMSQGALDAFEQTFTEILNATLILQVVDPTDREWRVKKRY
                     VDELLDSCGVAVGSVEVLYSKRDLLTGHPPDGGERCYAAGDRGDVRRLKRSLFERLFG
                     ETAPA"
     gene            complement(497..>1728)
                     /locus_tag="PKH10_12965"
     CDS             complement(497..>1728)
                     /locus_tag="PKH10_12965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNT29074.1"
                     /translation="EKKIASLKSGVMPIYEPGLDAVVARNVANERLSFTTDMKRTVET
                     ATVIFIAVGTPPKDDGSADLQYVLAVAKEIGRHMNKYTVIVDKSTVPVGTGQLVRKTI
                     ADELKGRGVSHEFDVVSNPEFLREGHAIHDFTHPDRVVIGTASKRAEEAMQQVYRVLF
                     LNQTPFVFTTIETAEMIKYASNAFLAAKISFINEMALLAEKVGANVQDIAKAMGMDGR
                     ISPKFLHAGPGYGGSCFPKDTKAIYDIGRKHGVELMVIKAAIEANEKQKQKMVEKILA
                     AMGSVKGKTFAVLGLTFKPDTDDMRDAPSLTIIPALVKAGATVRTFCPQGFHEAKWRL
                     ADHEKAIAYCADSYEAVTGADAVIILTEWNQFRGMDLTKLRSLMKDRFFFDLRNVYSR
                     DQQVRELFDYHGVGLPR"
BASE COUNT          292 a          521 c          548 g          367 t
ORIGIN      
        1 ccaaccggcc gaccgtggcc atcgtcggct acaccaacgc ggggaaatcg acgctgttca
       61 acgcgatcgt cggcgagaag gtggtgcggg gatccgacga gttcttctcc tcgatagacc
      121 cgaagatacg gctggtcacg ctcttcggtc gcaagttctt cctgctcgac acggtcgggt
      181 tcatcgaagg gatgtcgcag ggggcgctcg atgcctttga acagaccttc accgagatac
      241 tgaacgcgac gctcatcctg caggtggtcg atccgaccga tcgcgaatgg cgcgtcaaaa
      301 aacggtatgt ggacgaactg ctcgattcgt gcggggtggc ggtcggcagt gtcgaggtgc
      361 tttactcgaa acgcgacctg ctcaccggcc atccccccga cggcggcgaa cggtgctacg
      421 cggcgggcga tcggggcgac gtccggcgac tcaaacgatc cctgttcgaa cgacttttcg
      481 gagagacggc gcccgcctag cgcggcagac cgacaccgtg ataatcgaac agttcccgga
      541 cctgctggtc gcgggagtag acattgcgca ggtcgaagaa gaaccggtcc ttcatgagcg
      601 accgcagttt cgtgagatcc atgccgcgga actggttcca ttcggtcagg atgatcactg
      661 cgtcggcccc ggtgaccgcc tcgtaactgt cggcgcagta ggcgatcgcc ttctcatgat
      721 cggccagtcg ccatttcgcc tcgtggaacc cctgcgggca gaaggtccgc accgtcgcgc
      781 ccgccttgac gagcgccggg atgatggtca gcgacggggc gtcgcgcatg tcgtcggtgt
      841 cgggcttgaa ggtgagcccg aggacggcga atgtctttcc ctttacgctt cccatagcgg
      901 ccagtatctt ctcgaccatc ttctgtttct gcttctcgtt ggcctcgatg gccgccttga
      961 tgaccatcag ttcgacgccg tgtttcctgc cgatgtcgta gatggccttg gtgtccttcg
     1021 ggaaacagga gccgccgtag ccgggcccgg cgtggaggaa cttgggcgat atgcggccgt
     1081 ccatccccat cgccttggcg atgtcctgca cattggcccc gaccttctcg gcaaggagcg
     1141 ccatctcgtt gatgaacgat atcttcgccg cgagaaaggc gttcgacgcg tactttatca
     1201 tctcggccgt ctctatcgtc gtgaagacga acggcgtctg attgaggaac aggacgcggt
     1261 agacctgttg catcgcctcc tcggcgcgct tgcttgcggt gccgatgacg acgcggtcgg
     1321 ggtgggtgaa gtcatggatg gcgtgacctt cgcgcaggaa ttcggggttc gaaacgacat
     1381 cgaattcatg cgaaacgccg cgtcccttga gttcgtcggc gatcgtcttc ctgaccagtt
     1441 ggccggtccc caccgggacg gtcgatttgt cgacgatgac ggtgtatttg ttcatgtggc
     1501 ggccgatctc cttggcgacc gcgagtacat actgcaggtc ggccgagccg tcgtccttgg
     1561 gcggcgtgcc gaccgcgatg aatatcaccg tggcggtctc gaccgtgcgc ttcatgtcgg
     1621 tcgtgaacga aagccgttcg ttggcgacgt tgcgcgcgac gaccgcgtcg agtcccggct
     1681 cgtagatcgg catcacgccg cttttcagcg atgctatctt cttctcgt
//
LOCUS       DAOQUE010000557         1723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_92360_length_1723_cov_3.242206, whole genome shotgun sequence.
ACCESSION   DAOQUE010000557 DAOQUE010000000
VERSION     DAOQUE010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92360_length_1723_cov_3.242206"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..>1723)
                     /locus_tag="PKH10_12970"
     CDS             complement(28..>1723)
                     /locus_tag="PKH10_12970"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29075.1"
                     /translation="ICGNGIVDPGETCDDGQQNGDYNHCNITCTGLGPRCGDNITQGG
                     NGEQCDDGNTNDCDACHNDCTTYTGNICSDGHACGTEQCDDANTVNTDACKNDCSLNV
                     CGDGVTRTSYEEMLLRLDLNEGAGATAYDSSGNANHCAISGASWVAGRFGSALSFDGV
                     NDTITCPANLAVNDFTIEAWVKPTTTHELDTESITGTGGISGQKYVLFPGIHVGMDAG
                     MGISVGTNGISVYEHGDSYMPPLAVYAGVISSNEWSRITVTYTNKQPRIYLNGTLVRT
                     GLSSPRSNVYASGLAGGGAYGYFPGQMDGIRIYDRALTAEEVATGLVEDCDDGNTSDC
                     DSCHANCTVNTGCGDGRICGAEACDQGGGNAANGDGCSSSCAVESGWSCSGTPSICTV
                     LPVIIGTGTTTQGYPLNTYYSYVYSAAIYTSSEIGASRTITKLAWYPTVTSSCSRNLK
                     IYLKPTTSSALSAVTWSSLTSGATLVYNTTITVPTANQWIDYDITDFYYSNSSNLLVL
                     VENIYGGGFGYCGGETSPSVRYSPVSSAHEFWRYDDEPPIGTGTVTSNRPNIRISF"
BASE COUNT          378 a          459 c          484 g          402 t
ORIGIN      
        1 acctgttatc ttacacccct ttacctgtca gaaagatatg cggatattcg ggcgattgct
       61 ggtgacggtt ccggtaccga tcggtggctc atcatcgtat cgccagaatt cgtgggcgga
      121 agagacggga ctgtaccgca ccgacggaga ggtttcgccg ccgcagtaac cgaaaccgcc
      181 accgtagatg ttttccacca ataccagcaa atttgaagag ttgctgtagt aaaagtcggt
      241 aatgtcatag tcgatccact gattcgccgt tgggacggtg atcgtcgtgt tgtagactag
      301 tgttgcgccc gaggtcaggg aggaccatgt aacggcagaa agggcagagg aggtggtcgg
      361 tttgaggtat atcttgagat tacgggaaca agaggaggtg acggttggat accacgcaag
      421 ttttgtgatg gtgcgggatg ctccgatctc ggatgaggtg tatatggccg ctgaatagac
      481 ataggagtag taggtgttca gcggataccc ctgtgtggtt gtccccgtac cgataatgac
      541 cggcaaaacg gtgcaaatgg acggcgttcc ggaacaggac catcccgatt cgacagcgca
      601 ggaactacta cagccatcgc cattagcggc attgcctccg ccttggtcgc aggcctcggc
      661 accacagatg cgaccatcgc cacatccggt attgacagtg cagttggcgt ggcaggaatc
      721 gcagtcacta gtgttcccgt cgtcacagtc ctccaccaag ccggtcgcca cctcttcggc
      781 ggtgagggcg cggtcgtata tccgtatacc atccatttgt cccgggaaat atccgtaggc
      841 gcctccacct gccaagccgg aagcgtatac atttgatcga ggagaagaca ggccggtacg
      901 gaccaaggta ccgttcagat agatgcgagg ttgcttgttc gtgtatgtga ccgtgatccg
      961 cgaccattca ttgctgctga tgacccccgc atacacggcc agaggaggca tataactatc
     1021 gccatgttcg tagaccgaga ttccgttcgt cccgaccgat atccccatgc cggcatccat
     1081 gcccacatga atgcccggga aaaggacata tttttgaccg gaaattcccc ccgtccccgt
     1141 gattgactct gtgtcgagct catgcgtggt tgttggtttg acccacgctt caatcgtgaa
     1201 gtcattgacg gcgaggttgg cgggacaagt gatcgtgtcg ttcacaccat cgaaactcaa
     1261 ggcgctgccg aaacgaccgg cgacccaaga ggcgccgctg attgcacagt ggttcgcgtt
     1321 gccggaagaa tcataggccg ttgctccggc tccttcgttc agatccaatc tgaggagcat
     1381 ttcttcgtag gaagttctcg tgacaccgtc gccacagaca ttgagggaac aatcattctt
     1441 gcacgcatcg gtgttcacgg tattagcgtc atcgcattgc tcggtaccgc aagcatggcc
     1501 gtcgctacag atgtttcccg tgtaggttgt gcagtcgtta tgacaggcat cgcaatcgtt
     1561 ggtgttgccg tcgtcacact gttcgccatt ccctccttgc gtgatattgt caccacaacg
     1621 agggcccaat cctgtgcagg tgatattgca gtggttgtaa tccccgttct gttgaccatc
     1681 atcacaagtc tcgccgggat caacgatgcc gttaccacag ata
//
LOCUS       DAOQUE010000558         1722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_92430_length_1722_cov_5.636473, whole genome shotgun sequence.
ACCESSION   DAOQUE010000558 DAOQUE010000000
VERSION     DAOQUE010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92430_length_1722_cov_5.636473"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PKH10_12975"
     CDS             complement(<1..1112)
                     /locus_tag="PKH10_12975"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT29076.1"
                     /translation="MTRAVLVSFLSFLWAMTLFASPKADATQRLFEAVGDMKLTDVAS
                     AIEDGADVNAVDDDGWPLFVTAVNSGQMGIIRLFLTKKELDLGLTGPDGKTAFMHAIV
                     MKNRTLAEILLQKGSSIHTPDGRGKTPLMYAAEVGDEKLVQLLLDKGADRNAKDEEGK
                     TALEYAFESRRKGAIDLLSKTDRLPVELVAAAQRGDGGAVRSLLAKGAPIDAKTADGK
                     PLMVMAVEKGFLDVLKALIDGKADVNGKYFKGSTLLMFAFHKGQLGAAELILRSGGEG
                     DLNLKYKEGKTALMMAIEEDRPSLVNLLMGKPFRINQTDSYGKTVLFYAVEKNNADLV
                     DRLLGLGADPTARQFEGKTAAQVAKDGSHPCKGGLV"
     gene            complement(1143..>1722)
                     /locus_tag="PKH10_12980"
     CDS             complement(1143..>1722)
                     /locus_tag="PKH10_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29077.1"
                     /translation="AFTADLVTWLSNDKVGIPIVDNAPRSMAQVITAMNAVNAKFIGL
                     DSSDGQVAMPYYEQLSIGTDSVDGDGYSFNQVISPDGTGMSQDVVDAVIELTANIAID
                     VSTLRKHVANTFGVSDTTKFITGISPTEFPNVNPGEKVTFEVTFQNTFHDNTSTETRL
                     FIAKIEVWGSGTKLDTRDCYILVPGKDASTPD"
BASE COUNT          298 a          525 c          525 g          374 t
ORIGIN      
        1 cagacgagcc cgcccttgca ggggtggctc ccgtccttcg ccacctgcgc cgccgtcttt
       61 ccctcgaact gccgcgcggt cgggtcggcg ccgagaccga gcagacggtc cacgaggtcg
      121 gcgttgttct tctcgacggc gtagaacagc accgttttgc cgtaggaatc ggtctggttg
      181 atcctgaacg gttttcccat gaggagattg acgagcgacg gccggtcctc ctctatcgcc
      241 atcatgagcg ccgtcttgcc ttctttgtat ttgagattga gatcgccctc gcctccgctc
      301 cgcaggatga gttcggccgc gccgagctgc cccttgtgga acgcgaacat gagaagcgta
      361 ctccccttga agtacttgcc gttcacatcg gccttgccgt cgatgagcgc cttcaggaca
      421 tcgagaaatc ccttttcgac cgccatcacc atgagcggtt tgccgtcggc tgtcttggcg
      481 tcgatcggcg cccccttcgc aagcagggaa cgcaccgcgc ccccgtctcc ccgctgggcc
      541 gccgcgacaa gttccaccgg cagacggtcg gtcttcgaca gcaggtctat cgcccccttg
      601 cggcgcgatt cgaatgcgta ctcgagcgcc gttttgccct cctcgtcctt ggcgttgcgg
      661 tcggctccct tgtcgagcag gagttgcacc agtttctcgt caccgacctc ggcggcgtac
      721 atgagcggcg tcttgccgcg gccgtcgggc gtatggatgc tggaaccttt ctggaggagt
      781 atctcggcga gcgtccggtt cttcatcacg atggcgtgca tgaaggcggt ctttccgtcg
      841 gggcccgtga ggcccagatc gagttccttc ttggtcagga acagccggat gatacccatc
      901 tggcccgaat tgaccgcggt gacaaagagc ggccagccgt cgtcatctac cgcgttgaca
      961 tcggcgccat cctctatcgc cgacgcgaca tcggtgagtt tcatgtcacc gaccgcctcg
     1021 aacagccgct gggttgcgtc ggctttcggg gaggcgaaca atgtcattgc ccacagaaaa
     1081 gaaagaaagg acacaaggac ggcacgggtc atggcttcct ccggaacgat atcgtcatgg
     1141 cgtcagtcgg gcgtactggc atccttgccc ggcacgagga tgtagcagtc gcgggtgtcg
     1201 agtttcgtgc cacttcccca cacctctatc ttggcgatga acagacgcgt ttcggtcgag
     1261 gtgttgtcat ggaaggtatt ctggaaggtg acctcgaagg tgaccttctc gcccgggttc
     1321 acattgggga actccgtcgg ggagatgccg gtgatgaact ttgtcgtgtc ggagacaccg
     1381 aacgtgttgg cgacatgttt gcgcagggtc gagacatcga tcgcgatgtt ggcggtcagt
     1441 tcgatcaccg cgtcgacgac atcctgcgac atgccggtgc cgtcgggact tatcacctga
     1501 ttgaaggaat agccgtcgcc gtcgaccgaa tcggtgccga tcgatagttg ctcgtaataa
     1561 ggcatcgcta cctgcccgtc gctcgaatcg agcccgatga acttggcgtt caccgcgttc
     1621 atcgccgtga tgacctgcgc catagaacga ggggcattgt cgacgatggg gatgccgact
     1681 ttgtcgttgg aaagccaagt aaccaagtcg gccgtgaagg cc
//
LOCUS       DAOQUE010000559         1722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_92439_length_1722_cov_3.837433, whole genome shotgun sequence.
ACCESSION   DAOQUE010000559 DAOQUE010000000
VERSION     DAOQUE010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92439_length_1722_cov_3.837433"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PKH10_12985"
     CDS             complement(<1..327)
                     /locus_tag="PKH10_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29078.1"
                     /translation="MRNSAFIVACTLLLIAACGGTSTTGENPDTSITIDQIVGVWKVD
                     SVQVLKDTCHFPNMDLDDELGTYSYIEKADATHVNVYDCGTDATCANKSTPSETYEYA
                     AGRITIP"
     gene            complement(355..1245)
                     /locus_tag="PKH10_12990"
     CDS             complement(355..1245)
                     /locus_tag="PKH10_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29079.1"
                     /translation="MKRLNWLMSLALIVVAIGCSSIPKKELIVLQKSKIAGRDILTVV
                     MSDLKKKEFAALSAGIVSAAGSPKIVPIPDGVDRYVEEAIEEAMANRSAPVVKEIVDI
                     LSMTLSFDTILIVKTYATDVTDRFIGIEIKTYPDSMHLLATLFDVKSGGLLGAIASPV
                     RVLDQSSRIADIGEGARGAIAELMEEVSLDEPKKEEKGGDDPKKLLRACKKGDGAACG
                     KIGDLFMEKTDIRDHRRKAEDFYRKGCDLDDKTSCDKYDKLIAGDDGGDDEEDEDRPA
                     KRSKRRSYDEDEEEEEEEDL"
     gene            complement(1363..>1722)
                     /locus_tag="PKH10_12995"
     CDS             complement(1363..>1722)
                     /locus_tag="PKH10_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNT29080.1"
                     /translation="PTVITAQEAKEKYHGKPDVVFIDNRPDPDFCAGHIPGAVNLTYF
                     APDTPGNRLTAEALKAFAGKKLVFYCKGVLRAKNAVEAARSWGIDPKLLFWFKDGFPA
                     WQKQGYPVEKMDDACPI"
BASE COUNT          260 a          533 c          523 g          406 t
ORIGIN      
        1 cgggatcgtt atccggccgg cggcgtactc ataggtctcc gacggagtgc tcttgttcgc
       61 gcaggtcgcg tcggtgccgc agtcgtagac attgacatgg gtcgcgtcgg ccttttcgat
      121 gtaactgtag gtgcccagtt cgtcgtcgag gtccatgttg gggaagtggc aggtgtcctt
      181 gagcacctgt accgaatcga ccttccatac gccgacgatc tggtcgatgg tgatgctggt
      241 gtcggggttc tcgccggtcg tgctcgtgcc gccgcacgcg gcgatgagca gcagggtgca
      301 ggcaacgatg aacgcgctgt tcctcatggt actatcctcc gggtgtgtgg ggtcctaaag
      361 atcttcctct tcttcctctt cctcgtcctc gtcgtatgaa cgcctcttcg accgcttcgc
      421 gggccggtcc tcatcctctt cgtcgtcccc cccgtcgtcg cccgcgatga gtttgtcgta
      481 cttgtcgcag gaggtcttgt cgtcgagatc gcaccccttg cggtagaaat cctcggcctt
      541 gcggcggtgg tcgcggatat cggtcttctc catgaagagg tcgccgatct taccgcaggc
      601 ggccccgtcg cccttcttgc aggcccgcag gagtttcttg gggtcgtcgc cgcctttctc
      661 ctcctttttc ggctcgtcga gcgatacctc ctccatcagt tccgctatcg cgccccgggc
      721 cccttcgccg atgtcggcga tgcgcgaact ctggtccagc acccgcaccg gcgacgctat
      781 cgcgccgagc aggcccccgc tcttcacatc gaaaagggtc gcgaggaggt gcatgctgtc
      841 ggggtaggtc tttatctcga tgccgatgaa acggtcggtc acatcggtcg cgtaggtctt
      901 gacgatcagt atcgtgtcga acgagagcgt catggaaagt atgtcgacga tctccttgac
      961 gaccggcgcg gagcggttgg ccatcgcctc ctcgatcgcc tcctcgacgt agcggtcgac
     1021 cccgtcgggg atggggacga tcttgggcga tccggcggcg ctcacgatgc cggcgctgag
     1081 ggcggcgaac tcctttttct tgaggtccga catcacgacg gtcaggatgt cgcgccccgc
     1141 tatcttgctc ttctggagta cgataagttc ctttttaggt atggatgagc acccgatcgc
     1201 gacaacgata agtgctaatg acatcagcca gttgagtctt ttcatccgtt cacctcctga
     1261 gggtcaggtc atcggatcgg tatcgcgcct ccaacgcatc cggggtggac cattatcctc
     1321 ttttcgggag gtcaaggcaa actttttttt gagaaatgtg atctatatcg ggcaggcgtc
     1381 gtccatcttc tcgaccgggt agccctgctt ctgccacgcg gggaacccat ccttgaacca
     1441 gaacagcagt ttcgggtcga tcccccacga ccgggccgcc tcgaccgcgt tcttcgcccg
     1501 gagcaccccc ttgcagtaga agaccagttt cttccccgcg aaggctttca gcgcctcggc
     1561 ggtcagccgg ttccccgggg tgtcgggggc gaaataggtg aggttcaccg cgcccgggat
     1621 gtggccggcg cagaaatcgg ggtcgggccg gttgtctatg aacacgacat cgggcttgcc
     1681 gtggtacttc tccttcgcct cctgcgccgt gatgacggtc gg
//
LOCUS       DAOQUE010000560         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_92885_length_1717_cov_3.264140, whole genome shotgun sequence.
ACCESSION   DAOQUE010000560 DAOQUE010000000
VERSION     DAOQUE010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92885_length_1717_cov_3.264140"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PKH10_13000"
     CDS             <1..910
                     /locus_tag="PKH10_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29081.1"
                     /translation="LDDTENFMLDISSTATTVAPGDFTTVSITFDPTLEGSLASFVTI
                     ESDDPAGDYILNIGGAGLPTPVFPLLLDNYDGTGDLTYANDGVWSIAGGVYESYANGL
                     TTPEHNYASYDLTQSIADWTLDKSRGNEWFGWLKMNRTLNVDGWSTDHFSMAMVLAAD
                     NADFNDGTTKGYAVGFRNDGALVVFRFDAGITNANDVLPGNSTELVNSGYICTGSKDA
                     DGGVNVYVELKNGGKWAIYYKHGVQLSDTDARDKMKYTDGTATSAAVDETYTGTAYKY
                     AGWAHAHKASGDHKALMDNFGAGLTE"
     gene            937..1626
                     /locus_tag="PKH10_13005"
     CDS             937..1626
                     /locus_tag="PKH10_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29082.1"
                     /translation="MRTSLFALVCATLFVAACASDDADSDNENVPDDLVQTDHTGTVD
                     DAITADDASAPTDDTVTPTDTDEVVTDNDIATDDIATDDIAIDDILTDETVDENDDAD
                     VVVTPDSDLDPEAAIACCVEIVKEWWRCAVEVYKTEQADACKTCVEGLAACTDFLPLD
                     AEWACAASCNDICPPGTVYENDDTVGKGLGQSRRAVAEEYCTWMVDSACAYEPADFTG
                     LGSTEPLEACF"
BASE COUNT          366 a          580 c          491 g          280 t
ORIGIN      
        1 ccttgacgac accgagaact tcatgctcga catatcctcg accgccacca ccgtcgcccc
       61 gggcgacttc acgacagtca gcatcacttt cgacccgacc ctcgagggct ctctcgcctc
      121 cttcgtgacg atcgagagcg acgacccggc gggcgactat atcctgaaca tcggcggcgc
      181 cgggctcccg acgccggtgt tcccgctcct gctcgacaac tacgacggca cgggtgacct
      241 gacctacgcc aacgacgggg tctggagcat cgctggcggc gtttacgaaa gttacgcgaa
      301 cgggctgacc acgcccgaac ataactacgc atcctacgat ctcacccagt cgatagccga
      361 ctggacgctc gacaagtcga gggggaacga atggttcggc tggctcaaga tgaaccggac
      421 gctcaatgtc gatgggtggt cgaccgacca tttctccatg gcgatggtgc tcgccgccga
      481 caacgccgac ttcaacgacg gcacgacgaa aggctacgcc gtcgggttcc ggaacgacgg
      541 ggcgctcgtg gtgttccgtt tcgacgccgg catcaccaac gcgaacgatg tcctgccggg
      601 aaattccaca gaacttgtca attccggcta tatctgcacc ggaagcaagg acgccgacgg
      661 cggcgtcaat gtctatgtcg aactcaagaa cggcggtaaa tgggcgatct actacaaaca
      721 tggcgtccaa ctctccgaca ccgatgcccg cgataagatg aagtacaccg acggtaccgc
      781 cacctcggct gcggtcgacg agacctacac cggcaccgcc tacaagtacg ccggctgggc
      841 ccacgcccat aaggcgagcg gcgaccacaa ggcgctcatg gacaatttcg gcgccgggct
      901 gaccgaatag caagaaaaga tacggaggtg accgacatgc gcacgtctct ctttgctctt
      961 gtctgcgcta cgcttttcgt tgccgcctgc gcttccgatg atgccgatag cgacaacgag
     1021 aatgtgcctg atgaccttgt ccagaccgac catacgggga cggtcgatga cgccataacg
     1081 gcggatgacg cgtcggcccc gaccgatgac accgtcacac cgaccgacac cgacgaggtc
     1141 gtgaccgaca acgacatcgc caccgacgac atcgccaccg acgatatcgc catcgatgat
     1201 atcctcaccg acgaaacggt cgacgagaac gacgatgccg atgtcgtggt gactcccgac
     1261 agcgatctcg atccggaggc ggcgatcgcc tgctgcgtcg agatcgtgaa ggaatggtgg
     1321 cgctgcgccg tcgaggtcta caaaacagaa caggccgacg cctgcaagac ctgcgtcgag
     1381 ggactcgccg cctgcaccga cttcctgccg ctcgacgccg agtgggcctg cgccgcatca
     1441 tgcaacgaca tctgcccgcc cggcacggtc tacgagaacg acgacaccgt tggcaagggg
     1501 ctggggcagt cgcgccgcgc ggtcgccgag gaatactgca cttggatggt cgacagcgcc
     1561 tgcgcctatg agccggccga cttcaccggg cttggctcta ccgagccgct cgaggcctgt
     1621 ttctagcatg ttcggctcat tattactcgc cgcaacactg ctctccaccg ccgaaacgac
     1681 cgcatccgcg cccgacgaca ccgccatcac gcagacc
//
LOCUS       DAOQUE010000561         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_93258_length_1713_cov_2.920989, whole genome shotgun sequence.
ACCESSION   DAOQUE010000561 DAOQUE010000000
VERSION     DAOQUE010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93258_length_1713_cov_2.920989"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKH10_13010"
     CDS             <1..210
                     /locus_tag="PKH10_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29083.1"
                     /translation="AMPQCRNVPSSHGSAPTLLYKGKGGGAQKNGGWGTTEVSLPFGM
                     ETKAKIDCRLDCATNSGARRLGKPG"
     gene            241..>1713
                     /locus_tag="PKH10_13015"
     CDS             241..>1713
                     /locus_tag="PKH10_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29084.1"
                     /translation="MPSMFTSIMRNQTQALLLCLVLSIPRFASADGVTIITHGFAPDF
                     LSLYSPDNPEWVDRMESAIENRLYEHFPNPRVSTGLVKFTQINSLLYTYNFQWITNHF
                     SDSSSGEIVIIVDWADLAGLDEAVPTQTIAENAFAVLPVQAYQLPIHLIGHSRGTSVN
                     TRLCELIGEEGIWVDQFTTLDPHPVAAFNDETVKVHENVLFADNYWRDDGGLLRTKDI
                     DPKESDPQLREIGIPDVNGRSIDGAFNRNLGALDDEDDPDYGYYHEHSDTHLWYHGTI
                     DTALYAFDGEHFVLPEMRYGSVWWNEHEDEGHNAGYIYSRIAGRGDDYRLTTDPPIVD
                     GYHGIHGGGGSRYLLDWTNAVWPNVFKFEIEANGAPLQGWGPHQIQVGQPLDLKYEAQ
                     DNDSHFTVSLFVDEDRNPYNGYLVSLDHLTDTHAATGQGWYHPTKTWNTGGFVSAGTD
                     CYVYAKITDGTHTRYFYATPKLHFVAVPTYTISSSAGSHGS"
BASE COUNT          452 a          408 c          430 g          423 t
ORIGIN      
        1 gctatgcccc aatgtcgcaa tgtcccctct tcccacggga gcgcccctac gcttctgtac
       61 aagggaaagg gaggaggtgc acagaagaac ggtggctggg gcactacgga agtgtccttg
      121 ccgttcggaa tggagacgaa ggcgaagata gattgcagat tggattgtgc cacgaactca
      181 ggagcccggc gtctggggaa accgggttag gcactggaaa gagttgagga ggacaggcat
      241 atgccatcta tgttcacatc aataatgaga aatcaaacgc aggctcttct tctgtgtctc
      301 gttctttcta ttcctagatt tgcgtctgcg gatggcgtca ccatcattac gcatggattc
      361 gctccagatt ttcttagcct ttatagtccc gacaatcctg aatgggttga tcgaatggaa
      421 tccgccatag agaataggct atatgaacac tttcccaacc cccgtgtcag taccggcttg
      481 gttaaattca ctcagatcaa ctctcttttg tatacataca attttcaatg gataacgaat
      541 cacttctcag attcttcatc aggagaaata gtaatcatag tcgattgggc cgatctcgcc
      601 ggcttggatg aagcagtacc aacacaaacc atcgcggaaa acgcattcgc agtgcttccc
      661 gtccaagcct accaactacc cattcatctg atcggccata gtcgtggaac ttcggtaaat
      721 acacggctat gcgaattgat cggagaagaa ggtatatggg ttgaccagtt tacgacactt
      781 gaccctcatc ctgttgcagc attcaatgat gaaacagtca aggtccatga gaatgtatta
      841 tttgccgaca attactggcg tgacgatggt ggtttgttac gaacgaaaga tattgatccc
      901 aaagaatcgg atccccaact gagagagatc ggtatcccgg atgtcaatgg acgatcgatt
      961 gatggggcat tcaatcgtaa tcttggcgca ctggatgacg aagatgatcc agactacggg
     1021 tactatcatg aacactctga cacgcatcta tggtaccatg ggacaatcga caccgcactc
     1081 tatgcctttg atggcgagca tttcgtttta ccagaaatgc gatatggttc tgtttggtgg
     1141 aacgagcatg aagacgaagg gcacaatgcg ggatacattt atagtcgtat tgcaggaaga
     1201 ggcgacgatt accggctgac aaccgatcca ccaatagttg acggttacca cggtattcac
     1261 gggggcggtg gttcgcgtta cctgcttgat tggaccaacg ccgtatggcc taatgtcttt
     1321 aagttcgaaa ttgaagccaa tggtgctcct ctacagggat gggggcctca tcaaatacaa
     1381 gtgggccaac cacttgatct caaatatgaa gcacaagata acgatagcca ttttaccgtg
     1441 tccttgttcg ttgacgaaga caggaacccg tacaatggct accttgtgtc cctcgatcat
     1501 ctgactgaca cccatgcggc cacaggacag ggttggtacc acccgacaaa aacatggaat
     1561 acgggagggt ttgtttcagc ggggaccgat tgttatgtgt atgcgaagat cactgatggg
     1621 acacatacgc gatacttcta cgctacgccg aagcttcact tcgtcgcggt tccgacctat
     1681 acgatttcat cgtcagcggg ttcacacgga tcg
//
LOCUS       DAOQUE010000562         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_93323_length_1712_cov_4.885938, whole genome shotgun sequence.
ACCESSION   DAOQUE010000562 DAOQUE010000000
VERSION     DAOQUE010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93323_length_1712_cov_4.885938"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..954
                     /locus_tag="PKH10_13020"
     CDS             127..954
                     /locus_tag="PKH10_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29085.1"
                     /translation="MKRRHLIPFAILLAAVTCYLWWRFILLIPEADTLRELVTSLKQR
                     DTEGRALIILYPASEFTAFLPAIDPLKEDILVLPRRFETPPEDALLKKYSSFLLVAPS
                     RFPVTPWDTYRWRPERITESGRFTAYRLSPDRHATYTATSGGRGGNPFLRQCPDGQPF
                     TGMDVVRSGAGFSILSIRCGGNDIALKEPRPTGASISLRCPGDRRVTGLAGTADHHLR
                     GIALSCGAPDGSDEVLSVTAGNMKESRSLASCRRKSATGIFGRRGSLTDAIGLICER"
     gene            965..1501
                     /locus_tag="PKH10_13025"
     CDS             965..1501
                     /locus_tag="PKH10_13025"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNT29086.1"
                     /translation="MKMVREETILKTRIFTVAHVHLEKKSGDPVIHAVIRFVETVSVL
                     PVTKSGGIILERQFRSAVGDWVIEAPAGKIDPGESPEATVRREVEEEIGHRVIELREL
                     FEGWVSCGYTDEYMRYFAATVEPIPAMERTHFPDHDEEIETLAVTLDEALAMIERREI
                     VDAKTIALILAHDREKKS"
     gene            1485..>1712
                     /locus_tag="PKH10_13030"
     CDS             1485..>1712
                     /locus_tag="PKH10_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29087.1"
                     /translation="MKKSPEKRIATLYRVLRDHFGFQHWWPGETRFEIVVGALLTQNC
                     AWSNVEKAIANLKAARSLSLGAMQRLPRGELE"
BASE COUNT          353 a          539 c          509 g          311 t
ORIGIN      
        1 ccacgacatc aagatgttct actggatggc cgacagttcc cggcactccg aggaggcaaa
       61 gataaagaaa cacatcctgc atcaggagac cgccgaaacg aaatggatgc ttgtgcggga
      121 acggaaatga agcgtaggca tcttatcccg ttcgcaatcc ttctggccgc cgtcacttgc
      181 tatctctggt ggcgtttcat cctcctcata cccgaggccg atacgctccg ggaattggtg
      241 acctcgctta aacaacgcga tacggagggc cgggcgctca tcatcctcta tccggcaagc
      301 gagttcacgg cgttcctccc cgcaatcgac ccgctgaagg aggatatcct cgtcctgccg
      361 cgccggttcg aaacgccacc cgaagatgcg ctcctgaaaa aatactcctc ctttctgctg
      421 gtggctcctt cccgttttcc ggtaacgcct tgggacacct accgctggcg ccctgaacgg
      481 ataacggagt caggccgttt caccgcgtac cggctctccc ccgaccgcca tgccacctac
      541 actgcgacct ccggcggacg cggcggcaac ccgttcctgc gtcaatgccc cgacggacag
      601 cccttcaccg ggatggatgt cgtccggagc ggcgccgggt tctctatcct ttccatccgg
      661 tgcggcggga acgatatcgc gcttaaagaa cctcgtccga ccggcgcttc gatatcattg
      721 cgatgccccg gggatcggcg agtgaccggt cttgccggga ccgccgatca ccacctccgc
      781 ggcatcgccc tttcctgcgg cgcccccgac ggatcggacg aggtactttc ggtaacggcg
      841 ggtaatatga aggaaagccg atctcttgcc tcctgccgcc gtaaatcggc gaccgggata
      901 ttcggtcggc ggggatcgct taccgacgct atcggcctga tatgcgaacg ctgatggagg
      961 cctcatgaag atggtacgcg aagaaacgat cctgaagacc cggatattca cggtggccca
     1021 tgttcatctg gagaaaaaga gcggcgatcc ggtcatccac gccgtcatcc ggttcgtcga
     1081 gaccgtatcg gtcctgccag tcacgaaaag cgggggcatc atcctcgaac ggcaattccg
     1141 gtcggcggtc ggcgactggg tcatcgaggc gccggctgga aagatagacc ccggcgaatc
     1201 gcccgaagcg acggtgcgtc gcgaggtcga ggaggagatc gggcaccgcg tgatcgaact
     1261 gcgggaactc ttcgagggct gggtctcctg cggctatacc gacgagtaca tgcgctactt
     1321 cgccgcgacg gtcgaaccga taccggcaat ggaacgcacg cattttcccg accacgacga
     1381 ggagatagag acactcgccg tgacgctcga cgaggcgctc gcgatgatcg agcgccgcga
     1441 gatagtggat gctaaaacga tagcgctcat actggcccac gaccgtgaaa aaaagtcctg
     1501 agaagcgcat cgccaccttg taccgcgtac tgcgggacca tttcggcttc cagcattggt
     1561 ggcccggaga aacgcggttc gagatcgtcg tcggggcact cctcacccag aactgcgcat
     1621 ggagcaatgt cgagaaggcc atcgcgaacc tgaaagcggc gcggtcactc tcgctcggcg
     1681 cgatgcagcg attgccgcga ggggaactcg ag
//
LOCUS       DAOQUE010000563         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_93779_length_1707_cov_3.463680, whole genome shotgun sequence.
ACCESSION   DAOQUE010000563 DAOQUE010000000
VERSION     DAOQUE010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93779_length_1707_cov_3.463680"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PKH10_13035"
     CDS             <1..578
                     /locus_tag="PKH10_13035"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNT29088.1"
                     /translation="SAAIPMPGFVVPNKVGEYYVGVLDTGNIGSSQGATVDEVLRNTG
                     AFKLFSVTMPPPVGKAQLNIYTYPSDATIYIDGEKAGVGSVIGKNVNPGIYQITAKKF
                     LYEDETVIVTIGVGEVMPVELTLNPIIDSDDMTTIAVYAGAGILLAGVAYVVINKRAR
                     ARAIELGRTAWDKSKKAYGRAGELYAIARDA"
     gene            581..1060
                     /locus_tag="PKH10_13040"
     CDS             581..1060
                     /locus_tag="PKH10_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29089.1"
                     /translation="MYEIGKRVFGTQVFKTYDPQVSIDMVPGVTYQAVFDADLTTSGW
                     EDRVYQDLVEECSRHGCELTYFGVCYPNKKIIAQWQYHGPVPGFQSTILPAIPAIGAG
                     AVVMAILGVIGVSIGGVSFFKLSELYEKNPLGIGLVVGGVVLVGALLAFGYASRSVR"
     gene            complement(1241..1597)
                     /locus_tag="PKH10_13045"
     CDS             complement(1241..1597)
                     /locus_tag="PKH10_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29090.1"
                     /translation="MISADENRDKVRETLRGRLQGRGLPVGDTVFTGTGTEAKVTDFD
                     TSMIKQIYLVTEQNCPGCHDAMEEFLDDIQDGTLRVVDVGDDKGFEIVQALNLVAVPA
                     LVIELVGGGYLEFKRT"
BASE COUNT          362 a          519 c          480 g          346 t
ORIGIN      
        1 gtagcgccgc aattccgatg cccggattcg ttgtcccaaa caaagtcggc gaatactatg
       61 tgggcgtgct ggatactgga aacatcgggt cgtcccaggg cgccaccgtg gacgaagtgc
      121 tccggaacac cggtgcattc aaattgttct cggtgacaat gcccccgccc gtggggaagg
      181 cacagttgaa catctacacc tacccgagcg acgccacaat ctacatcgat ggcgaaaagg
      241 ccggtgtcgg gagcgtgatc ggtaagaatg tgaacccggg catctaccag atcaccgcca
      301 agaagttcct ctatgaagac gagaccgtga tcgtcaccat cggggtaggt gaagtcatgc
      361 cggtcgaact gacgctgaac cccatcatcg actcggatga catgaccacc attgccgtct
      421 acgccggcgc aggcatcctg ctcgcaggcg tcgcctatgt tgtgataaac aagcgggcac
      481 gcgctagggc aattgaactt gggcgcaccg cgtgggataa gagcaagaaa gcctacggcc
      541 gggctgggga actctatgcg atagcccggg atgcgtaacc atgtatgaga tcgggaagcg
      601 cgtcttcggg acacaggtct tcaagaccta cgacccccag gtcagcattg acatggtgcc
      661 gggcgtcacc tatcaggccg tgtttgacgc ggatctcacc acctccgggt gggaagaccg
      721 cgtctatcag gacctggtcg aggagtgttc ccggcatggg tgtgagttga cctacttcgg
      781 cgtgtgctat cccaacaaga agatcatcgc ccagtggcaa taccatggac cggtgccggg
      841 gttccagagc accatcctcc ccgcgatccc ggccatcggc gcgggagcgg ttgtaatggc
      901 aatcctaggc gtcattgggg tatctatcgg cggggtgtcg ttcttcaagt tgtcagagct
      961 ctacgagaag aaccccctcg ggatcgggtt ggttgtcggc ggggtggtgc tcgtcggggc
     1021 gctactcgca ttcggatatg cctcccggag cgtccggtaa cccaccgccc caattcaact
     1081 cttctttttc aggatccgca cggtcaacac atcgcccttt atactcgtat gaatcctctc
     1141 accgggcttg aggtcattca catagggttc caaaaaagag tttatatctt caagcctgtc
     1201 gggatcgagc cgtacaacaa cgactcgtcc ttcgtagaga tcaagttcgc ttgaactcaa
     1261 ggtatccgcc gcctacgagt tcgatgacca gggccgggac cgccacgagg ttgagcgcct
     1321 gcacgatctc gaaccctttg tcgtcaccca catccaccac ccgcagcgtc ccgtcctgaa
     1381 tgtcatcaag gaactcttcc atcgcgtcgt gacatcccgg gcagttctgc tcggtgacca
     1441 gatatatctg cttgatcatc gaggtatcaa aatcggtcac cttggcctcg gtgccggtcc
     1501 cggtgaagac ggtatcgcca accgggagcc ctctgccctg cagacgaccc cgcagcgtct
     1561 cccggacctt gtcccggttc tcatccgctg agatcataga cagcctcctg ccacgcagtc
     1621 atgaatgcct caaagaccgt cacgccctcg ttcacatgtg catcccaggc acgggccaga
     1681 atccaccggc gggcgtcgag aatcgac
//
LOCUS       DAOQUE010000564         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_93784_length_1707_cov_3.382567, whole genome shotgun sequence.
ACCESSION   DAOQUE010000564 DAOQUE010000000
VERSION     DAOQUE010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93784_length_1707_cov_3.382567"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKH10_13050"
     CDS             <1..306
                     /locus_tag="PKH10_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNT29091.1"
                     /translation="MPLCADIDLAGGQVIDLYFSEAVDALTADETTIFDNEVETGVVL
                     PGLFTIETVDGYPVVRFTATANFGAGTDYGLVVTKGVKGADGNPIVEEFRTFFTGTL"
     gene            complement(424..1338)
                     /locus_tag="PKH10_13055"
     CDS             complement(424..1338)
                     /locus_tag="PKH10_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29092.1"
                     /translation="MRWFLFPLCLIVAIACSDKAAKITPQDGDTIWSDSDLLADGDEL
                     LTDGPVNDTAKPDAADQGPLDVDTVMPDNDTVVNDIPVTPDEATDDDSLLSDDPVTDD
                     PVTDDFVPDNVQPDSDTAPFCGDGIKNGTEACDDGLANNGKYGYCNSICTGPGPRCGD
                     GNKDTGYEECDEGDALNGTYDHCADDCQGLGPRCGDGVTQDDEGELCDDGELLNGTYG
                     HCNDTCSGDGPYCGDGTVDTDNEECDKNKDQYCVGKDGSCGGGAQYRLRECDNDTCTW
                     TDWSNCGYSDGQDADPYPLDDGHCPDPC"
     gene            complement(1431..>1707)
                     /locus_tag="PKH10_13060"
     CDS             complement(1431..>1707)
                     /locus_tag="PKH10_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29093.1"
                     /translation="SVRYGFTCYLGADRWRGTPELPLSNVEHEKTRMDHALRLKALLE
                     YRFTDLFSLQAYYQYLRNFSNMGEDPADYENDTFDQHATGLMLRFVF"
BASE COUNT          337 a          508 c          496 g          366 t
ORIGIN      
        1 atgccgctgt gcgccgacat agaccttgcc ggcggacagg tgatagacct ctacttctcc
       61 gaggcggtcg atgctctgac cgccgatgag acgaccatct tcgacaatga ggtcgagacc
      121 ggcgtcgtac tgcccggact cttcaccatc gaaacggtcg acggctatcc ggtcgtccgc
      181 ttcaccgcga ccgccaactt cggcgcgggc accgattacg gtctggtggt gaccaaaggg
      241 gtcaagggcg ccgacggcaa cccgatcgtc gaggagttcc gcaccttctt caccggcact
      301 ctgtaaggat ctgtccctcc cacccccaag atagggcggg ccttctggcc cgcccgtttt
      361 ttttcatcga tgcaggagcc cctttcggta aaaaaaggaa ggggaagtgt atgggaaaag
      421 atatcagcag gggtcggggc agtgaccgtc atcgagcgga tagggatcgg catcctgccc
      481 gtcgctgtag ccgcagttcg accaatcggt ccatgtgcag gtatcgttat cgcattcacg
      541 gagccggtac tgcgcaccgc caccacaact gccatccttc cccacacaat actggtcctt
      601 gttcttgtcg cattcctcat tatcggtatc gaccgtgcca tcgccgcagt aggggccgtc
      661 cccagagcag gtatcattgc aatggccgta ggtaccgttc aacagttccc cgtcatcaca
      721 gagttcccct tcatcgtctt gggtgacgcc gtcgccgcag cgcgggccga gaccctgaca
      781 gtcgtcagcg caatggtcgt aggtgccgtt caacgcatca ccctcgtcgc attcctcata
      841 gccggtatcc ttgttgccgt cgccgcaccg gggaccggga ccggtgcata tactgttgca
      901 gtaaccgtat ttgccattgt tcgcgagacc gtcatcgcac gcctcggtac cgttctttat
      961 cccgtcacca cagaaaggag cggtgtcgga gtccggctgc acattgtcgg ggacgaagtc
     1021 atccgtcacg ggatcgtccg tcacggggtc gtcggagagc agggagtcat cgtcggtcgc
     1081 ctcatcgggg gtaacgggga tatcgttaac gaccgtatcg ttgtcgggca tgacggtgtc
     1141 gacatcgaga ggcccctgat cggcggcgtc gggtttcgcg gtgtcgttca ccggaccatc
     1201 ggtgagaagt tcgtcgccgt cggccagcag atcgctatcc gaccagatcg tgtcaccatc
     1261 ctgcggcgtt atcttggcgg ccttatcgct acaggcgatc gctacgataa gacaaagcgg
     1321 gaacaggaac catctcatga ggacctccct tctaacaaac catgctgaca ttgggcagta
     1381 tatcgctcat tctaccgaaa tcaagaggtt tttttccgaa gaggggtcgg ctagaacacg
     1441 aaacggagca tcaggcccgt tgcgtgctgg tcgaaggtat cgttctcgta gtcggcgggg
     1501 tcttcgccca tgttcgagaa attcctcagg tattggtaat aggcctgcag ggagaacagg
     1561 tcggtgaaac gatattcaag gagcgccttg aggcgcaggg catgatccat ccgggtcttt
     1621 tcgtgttcga cattagacag cggtagttcc ggtgtccccc gccaccgatc ggcccccaga
     1681 tagcatgtga agccatagcg caccgat
//
LOCUS       DAOQUE010000565         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_94239_length_1702_cov_6.402550, whole genome shotgun sequence.
ACCESSION   DAOQUE010000565 DAOQUE010000000
VERSION     DAOQUE010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94239_length_1702_cov_6.402550"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKH10_13065"
     CDS             <1..452
                     /locus_tag="PKH10_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29094.1"
                     /translation="TLYGWTESQFGSATDSDDLITIDLTTGAATLVGEFGLGDISWAT
                     GLAFDGDTLYVKFGYDIYTVDAVTGAGTYVADFTGILNFYDNDSGDDYDYLTNALAIS
                     DNGYYYTLQRDADNNSYLYAIDPDDWSVTFLGDLGIKNASALEFDKH"
     gene            635..>1702
                     /locus_tag="PKH10_13070"
     CDS             635..>1702
                     /locus_tag="PKH10_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29095.1"
                     /translation="MRIVTLIGAIVLTALLTVSCDETNKTNTPVSSVTVTATYVENGD
                     TIVVEGTNFGTTEPSGTLTKVTTSDALTDLPVELTVTDFSETRIAFLLPETFAEGNYV
                     LAGETANGGFVVTLVGLKGEQGDPGDPGDPGEDGVDGEDGVDGEDGADGASALVDVTD
                     EPAGENCANGGKKIATGLDDDGDGTLDEDEIDKVTYLCNAADGEDGADGVTTLVTVTD
                     EPVGTNCANGGKKIATGLDDDGDGTLDEDEIDHEEYVCNGEDGEDGADGNDALCYGNA
                     APTIGAITFDIAPGKNFRIGVPVTMFLETLDADGDTLDIVFSGMGASIVPTADEGVFE
                     VTPQMTGGPFMFGITVNDGCQT"
BASE COUNT          347 a          534 c          512 g          309 t
ORIGIN      
        1 gcaccctgta cggctggacg gagagccagt tcggttccgc gaccgattcc gatgatctta
       61 tcaccatcga cctgaccacc ggcgcggcga cgctggtcgg cgaattcggt ctcggtgata
      121 tctcctgggc gaccggcctc gcgtttgacg gcgacacgct ctatgtgaag ttcggctatg
      181 acatctacac ggtcgatgcg gtcacgggcg cgggcaccta tgttgccgac ttcaccggca
      241 tcctgaactt ctatgataat gatagcggag acgattacga ttatctgacc aacgctcttg
      301 ccatcagcga caacggttac tattacaccc tccagcgcga tgctgacaac aacagttatc
      361 tctacgccat cgaccccgac gactggagcg tcaccttcct cggcgacctc ggcatcaaga
      421 atgcctccgc cctcgaattc gacaagcact agacctttcc cgatctaatt cgacctcccc
      481 gttccggcgg tcatgatagg ccgccggaac ggatagccat attaagatgt tatgccatct
      541 tttgacttct ctgtcgtttt ttgagatact tcatcggctt gcgaaaccgc ctgttcccgc
      601 tgtcttttat aaaccggaac tcatggaggc atgcatgagg atcgtgacgc ttatcggggc
      661 catcgtgctg acggccctgc tgacggtctc ctgtgacgag accaacaaga cgaacactcc
      721 cgtttcctcg gtcaccgtga cggcgaccta tgtggagaac ggcgacacca tcgtcgtcga
      781 agggaccaac ttcggcacca ccgagccgag tggcacgctg acgaaggtca cgacgagcga
      841 cgcgctcacc gaccttcccg tcgaactgac cgtgaccgat ttcagcgaga cccgcatcgc
      901 gttcctgctt cccgagacct tcgctgaagg taactacgtc ctcgcgggcg agacggctaa
      961 cggcggcttt gtcgtgacgc tcgtcgggct caagggcgag cagggcgacc ccggcgatcc
     1021 gggcgacccc ggcgaggacg gcgttgatgg cgaggacggc gttgacggcg aggacggcgc
     1081 ggacggggcc agcgcgctcg tggatgtgac cgacgagccg gccggtgaga actgcgcgaa
     1141 cggcggcaaa aagatagcga ccggactcga cgacgacggg gacggcacgc tcgacgagga
     1201 tgagatcgac aaggtcacct atctctgcaa tgccgcggac ggtgaggatg gcgcggacgg
     1261 tgtcaccacc ctcgtgacgg tcaccgacga gccggtcggc acgaactgcg cgaacggcgg
     1321 caaaaagata gcgaccggcc tcgacgacga cggcgacggc acgctcgacg aggacgagat
     1381 cgaccacgag gagtatgtct gcaacggcga ggatggtgag gacggcgccg acggcaacga
     1441 tgcgctctgc tacggcaacg ccgccccgac gatcggcgcg ataaccttcg acatcgctcc
     1501 gggcaagaac ttccgcatcg gcgtcccggt gacgatgttc cttgaaacgc tcgacgccga
     1561 cggcgatacg ctcgacatcg tattctccgg tatgggcgca tccatcgtcc cgacggctga
     1621 tgaaggcgtc ttcgaggtga ccccccagat gaccggcggc cccttcatgt tcggcattac
     1681 ggtcaatgac ggctgtcaga cc
//
LOCUS       DAOQUE010000566         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_94931_length_1695_cov_3.152439, whole genome shotgun sequence.
ACCESSION   DAOQUE010000566 DAOQUE010000000
VERSION     DAOQUE010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94931_length_1695_cov_3.152439"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PKH10_13075"
     CDS             complement(<1..789)
                     /locus_tag="PKH10_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29096.1"
                     /translation="MRSFGVAFTVWSVMLFVAGCGNGTSILHKDSDTTAATDEIGLAD
                     EDTLAVDEDTPGMDEDDPAGDGAVEAETEGEDTVDETVDETTDETTDEVSDEATEAVT
                     EGNDAPDETADADTDADGSDPTDQSDQSDQDTITPDDDTADKATIYRIKLGEIAPNTV
                     TAIEGVVTGIRAAAKIFFVQVPEAAHDAQLGYTYSGVFVYAEGASGVTMPALGDRVRV
                     AGTVQNYYDNIQLATVTEVTVLGDGVIPEPVVVTIPVELVPPLEL"
     gene            complement(852..>1695)
                     /locus_tag="PKH10_13080"
     CDS             complement(852..>1695)
                     /locus_tag="PKH10_13080"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNT29097.1"
                     /translation="AGPRDAALEEQVQRRVGEISATLNYYSPGSLLTHLNTEGHTGPV
                     VVPGWFCQLLASSVNFTRETGGKFDITYKSEGYLWDIHRAEVPDPAAVRELLPLVGVE
                     NLVLDCEKNTVAFRKEGVKLDLGGIAAGYAADETRRIMERAGRKDFLVNYTGELVVCG
                     SKHGKPWTVGIRNPLKKEELIKTFNAPTDGCLSISTSGDYERYLEKDGKRYSHIIDPK
                     SGLPVEGAHSVTVSAADCMTADALATALSVGWRDRGFIAEMKQRYGLTVHMLTGDEVT
                     LTEY"
BASE COUNT          267 a          559 c          520 g          349 t
ORIGIN      
        1 gagctcgagc ggcggcacga gctcgaccgg gatcgtcacg acgaccggtt cgggaatgac
       61 gccatcgcca agcaccgtca cctcggtcac cgtcgccaat tggatgttgt cgtaataatt
      121 ctgcaccgtg ccggcaacgc gaacacggtc gccgagtgcg ggcatcgtca cccccgaggc
      181 cccctccgca tagacgaata caccgctgta ggtgtagccg agttgggcat cgtgcgccgc
      241 ctcggggacc tgcacgaaaa agatcttggc ggcggcgcgg ataccggtga ccaccccctc
      301 gatggcggtg acggtgttag gcgcgatctc accgagtttt atccggtaga tggtcgcctt
      361 gtcggcggtg tcgtcgtcgg gcgtgatagt gtcctgatcc gactggtccg actgatcggt
      421 cgggtcggat ccgtcagcgt cggtatcggc atccgccgtc tcgtcgggag catcgttccc
      481 ctcggtgacc gcctcggtcg cttcgtccga tacttcatcg gtcgtttcgt cggtcgtctc
      541 atcgaccgtt tcgtcgaccg tgtcctcccc ttcggtctcc gcctcgaccg ccccgtcgcc
      601 cgccggatcg tcctcgtcca tgcccggcgt atcctcgtcg acagcgagcg tatcctcgtc
      661 ggcaagcccg atctcgtcgg tcgccgcggt cgtgtcgctg tccttgtgca ggatgctcgt
      721 cccgttgccg cacccggcga caaacagcat caccgaccat accgtgaagg ccacgccgaa
      781 cgatctcatg gggtatctcc tccgaacgaa agatctgcgg tgattatagc gcggcggacg
      841 gcggaagcaa gttagtattc ggtcagcgtg acctcgtcgc cggtcagcat gtggacggtg
      901 agtccgtacc gttgtttcat ctccgcgatg aatccccggt cacgccagcc gaccgacagg
      961 gcggtcgcga gcgcatcggc ggtcatgcag tcggcggccg acaccgtgac cgaatgagcc
     1021 ccctcgaccg gcagaccgct tttcgggtcg atgatgtgcg agtaccgttt gccgtccttt
     1081 tccagatacc gctcgtagtc gcctgaggtc gagatgctca ggcacccgtc ggtcggcgcg
     1141 ttgaaggtct tgatgagctc ctcttttttg agcgggttgc ggataccgac cgtccacggc
     1201 ttcccgtgtt tactgccgca gaccaccagt tcgccggtgt agttgacgag aaagtccttg
     1261 cgccccgccc gctccatgat gcgccgcgtc tcgtcggcgg catagccggc ggcgatgccg
     1321 ccgaggtcga gtttcacccc ttccttcctg aaggcgaccg tgttcttttc acaatcgagc
     1381 acgagattct ccaccccgac gagcggcaga agttcccgca ccgcagcggg atcgggcact
     1441 tcggcgcggt ggatgtccca gagataccct tcgctcttgt aggtgatgtc gaacttgcct
     1501 cccgtttcgc gggtgaagtt caccgacgag gcgagcagtt ggcagaacca gccggggacc
     1561 accaccggcc ccgtgtgccc ttcggtattg agatgagtga gaagactgcc gggactgtag
     1621 tagttgagcg tcgccgatat ctcgccgacg cggcgttgca cctgctcctc caacgccgca
     1681 tccctcggac cggcg
//
LOCUS       DAOQUE010000567         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_95271_length_1691_cov_3.929095, whole genome shotgun sequence.
ACCESSION   DAOQUE010000567 DAOQUE010000000
VERSION     DAOQUE010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95271_length_1691_cov_3.929095"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PKH10_13085"
     CDS             complement(<1..1376)
                     /locus_tag="PKH10_13085"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNT29098.1"
                     /translation="MRSHARLFSFAEMLLLVAFSCTPTDDKNPGTGTYCESDLECGDT
                     AIYFCDKINNECRVKAGVDIDQGDIAGNDNGDTPSDQGGTEIDPTADDTGGVIPDMDY
                     AGLDCAPGTTEKCAYTGPAGTEDVGPCKAAVRLCKDNGTWGSCNGEIRPLYEICANGV
                     DEDCDGTVDNGTDLDGDGFTYCDTPPDCCETTDKCPDPGAVHPGASEVPDGVDNNCDG
                     TADEGVYDCDTGLDLNTKNPIDYAKSIGLCNGVTEAYVRLPSDQDGCNIQSNSLLSAL
                     GNVIKPKHGSSFLALSSGQAANPPSNTSFNQGTSSAAPGDWYQANGNKYPSSPSCGGG
                     TGTSGNANDGVMVKFVVQVPPTANSFSFNIYFLSIEYPSYICSTYNDFFVALLDSSYT
                     SADPNLQNPFDKNLAIDDMKNPVGINIAPNGLFKQCNNVSGDGWAVTSCVGTEELVGV
                     AVAVVTER"
     gene            1506..>1691
                     /locus_tag="PKH10_13090"
     CDS             1506..>1691
                     /locus_tag="PKH10_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29099.1"
                     /translation="MRSSFLTFFVALAGLVLFSVACSSGGNAANDNDGDDSENDLRDE
                     SDLTDEYLADEQPDLSDL"
BASE COUNT          314 a          456 c          537 g          384 t
ORIGIN      
        1 aatcgctctg tgaccacggc cactgctacg cctaccagtt cctcggtgcc gacgcaggag
       61 gtgaccgccc agccgtcgcc gctgacgttg ttgcattgct tgaagaggcc gttcggcgcg
      121 atgttgatgc cgaccggatt cttcatgtcg tctatcgcga ggttcttgtc gaatgggttc
      181 tgcaggttcg ggtcggcgct ggtgtaggaa ctgtcgagca gggcgacgaa gaaatcgtta
      241 taggtcgagc agatgtagct ggggtactcg atagagagga aatagatgtt gaaactgaac
      301 gaattggcgg tcggcgggac ctgcacgacg aacttcacca tgacgccgtc attggcgttg
      361 ccggaagtgc cggtacctcc gccgcaggag ggggaactgg gatacttgtt cccgttcgct
      421 tgataccagt cgcccggtgc ggccgaggag gtgccttggt tgaaactggt gttggatgga
      481 ggattagccg cctgtcccga tgaaagggcg aggaacgacg aaccgtgttt cggttttatc
      541 acgttaccga gtgccgacag cagagagtta ctttggatat tacatccgtc ctgatcgctc
      601 ggaagacgga cataggcctc ggtcacgccg ttgcacagac cgatggactt cgcatagtcg
      661 atcgggttct tggtgttcag gtcgagtccc gtatcgcaat cgtagacccc ttcatcagcg
      721 gtgccgtcgc agttgttgtc cacgccgtcg ggcacttcgc tggcgccggg gtgtaccgct
      781 cccgggtcgg ggcatttgtc ggtcgtttca cagcagtcgg gcggggtgtc gcagtaggtg
      841 aaaccgtcgc cgtcgaggtc ggtgccgttg tccacggtcc cgtcgcagtc ctcatcgacg
      901 ccgttggcgc atatctcgta gagcggccgg atctcgccgt tgcaggatcc ccaggtgccg
      961 ttatccttgc agagacgcac cgccgccttg caggggccga catcctcggt gcctgccggg
     1021 ccggtatagg cgcatttttc ggtggtgccg ggcgcgcagt cgagaccggc atagtccatg
     1081 tcggggatga caccgccagt gtcgtcggcg gtcgggtcga tctcggtgcc tccctgatcg
     1141 gagggagtat ccccgttatc gttcccggcg atgtctccct gatcgatatc gacgcccgcc
     1201 ttgacgcggc attcgttgtt gatcttatcg cagaagtaga tggcggtgtc gccgcattcc
     1261 aggtcgcttt cgcagtaggt gccggtgccg gggttcttgt cgtcggtcgg cgtgcaggag
     1321 aacgcgacga gcagcaacat ctctgcgaac gaaaataacc gtgcgtgaga tctcatgttt
     1381 tcctcctttg gcgaaacctg aagacaagta taagaacaaa aaaaataaaa atcaacatga
     1441 aagttcatga cggttgcttt ttaggagtaa cgcggtacac tgggtccgtt cacgcgggag
     1501 gcaccatgcg atcgtcattt cttacttttt ttgtcgcgct ggccgggttg gtgctttttt
     1561 ctgtcgcctg ttcgtcgggt ggcaatgccg cgaacgacaa cgatggcgac gacagcgaaa
     1621 acgacctcag ggacgaaagc gacctaacgg acgaatatct cgcggatgaa cagccggacc
     1681 tgtccgacct g
//
LOCUS       DAOQUE010000568         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_95562_length_1688_cov_3.488059, whole genome shotgun sequence.
ACCESSION   DAOQUE010000568 DAOQUE010000000
VERSION     DAOQUE010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95562_length_1688_cov_3.488059"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..199
                     /locus_tag="PKH10_13095"
                     /pseudo
     CDS             1..199
                     /locus_tag="PKH10_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020851905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
     gene            complement(142..798)
                     /locus_tag="PKH10_13100"
     CDS             complement(142..798)
                     /locus_tag="PKH10_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNT29100.1"
                     /translation="MLLSDDQRAVAAGFKYLIVSQVISAFLLIGIIFSYHATGVLNFD
                     LMRPDHFAALQAGAIAFFLMFVAVVAELKPFPANGWALDIYESAHPAFAGLFSVASAA
                     AALYAVDKLLVIGGPSWLPIATGIGIVTFIAMNLLALPQKNDRRLLGYSSVAQIGLIF
                     AVLGQRDILGDNHLFIAGGLLAAHAAAKAGLYWLSGLIASRDITGRYTNRFRSRRSAS
                     "
     gene            complement(1230..1586)
                     /locus_tag="PKH10_13105"
     CDS             complement(1230..1586)
                     /locus_tag="PKH10_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNT29101.1"
                     /translation="MALIAMAAGFILILIGFYGALTNRNILRMIVAFTVADTGVNIVM
                     VTVGYLRDRTAPILDNAVPAADAAARIIDPVPQALVLTAIVIGMGVTALMLAFAYRFY
                     EKKRTLDITKCTELKW"
BASE COUNT          339 a          496 c          584 g          269 t
ORIGIN      
        1 cgatccggat attgcggtct cgtcgtcgtt cggggcggac agcgcggtga tgctgcacct
       61 ggcgacgcgg atcaagccgg atatcaggat catcaccgtg gacaccgggt tcctgtttcc
      121 cgagacggtg gcgttccgcg atcagctcgc cgagcgtctg gacctgaacc tgttcgtgta
      181 ccggccggtg atgtcgcgcg acgcgatgag ccccgaaagc cagtagagcc ccgctttggc
      241 ggcggcgtgc gccgcaagga gcccgcccgc gataaagagg tgattatcgc ccagtatatc
      301 gcgctggccg agcaccgcga agatgagtcc gatctgggcg accgacgaat agccgaggag
      361 ccgccggtca tttttctgcg gcagggcgag caggttcatg gcgatgaagg tgacgatgcc
      421 gatgccggtc gcgatcggga gccacgacgg gccgccgatc accagcagtt tgtcgaccgc
      481 gtagagcgcc gccgccgccg aggcgaccga gaagagcccc gcgaaggcgg gatgggccga
      541 ttcgtagatg tcgagcgccc agccgttggc ggggaaaggc ttgagttccg ccaccacggc
      601 gacgaacatc aggaagaagg cgatcgcccc cgcctgcagg gcggcgaaat gatcggggcg
      661 catcaagtcg aagttgagca cgccggtcgc atgataggaa aagatgatgc cgatgagcag
      721 gaacgccgat atcacttgcg atacgatgag gtacttgaac ccggcggcca ccgcccgctg
      781 gtcatccgac aggagcatca gcccgccggt ggcgatgacg gcgagttcga agaagacgaa
      841 gaggttgaat atgtcgcggg tgagcacgat gccgttctgc gccatcacga acaccagcag
      901 caccgccatc gcggcgcgtc ccttctcgag gagcgcgcgc cggaggtaga gcgccgagaa
      961 aagacccgtc aggttgatga ggagcgtcag cgccgcctcg acgggtccca tccgcaggtt
     1021 gatcgcgaag gggggctgga ccccggcggt gaatatctcg accggttgcg cttcgtagac
     1081 ggcgaaggcc catacccact cggccgctat gaaggccatg aagccgagcg caaagagcgt
     1141 cacgccgtag gcggcggcac gccagcgttc gctgaagagt cccagcatga actcggcggc
     1201 cagcgccgcc gctatgatga cgatcgcgct taccatttaa gttcggtgca cttggtgatg
     1261 tcaagggtcc gttttttctc atagaaccgg taggcgaagg cgagcatcag tgccgtcact
     1321 cccatgccga tgacgatggc ggtcagcacc agcgcctgcg gcaccgggtc gatgatgcgg
     1381 gcggccgcat cggcggcggg gacggcgttg tcgaggatgg gggcggtgcg gtcgcgcagg
     1441 tagcctaccg tgaccatgac gatgttgaca ccggtgtcgg cgacggtgaa ggcgacgatc
     1501 atccgcagga tattgcggtt ggtgagcgca ccgtaaaaac cgatgagtat caggataaat
     1561 cccgccgcca tcgcgataag ggccatcaga gttcctccgt ttccgaaagg tgcgccagta
     1621 tcgaggagaa ctcggccccg accttgagcc cgatcagcga gtagatgagc ggtatcgcgc
     1681 cggccgag
//
LOCUS       DAOQUE010000569         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_96805_length_1675_cov_4.396296, whole genome shotgun sequence.
ACCESSION   DAOQUE010000569 DAOQUE010000000
VERSION     DAOQUE010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96805_length_1675_cov_4.396296"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKH10_13110"
     CDS             complement(<1..200)
                     /locus_tag="PKH10_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29102.1"
                     /translation="MRLHKKDNVLLHYLLESHENAGLSSTLSREGDMLTIQFSTPLSA
                     APYLDRLVDHGVDLDHRIIGSV"
     gene            complement(226..>1675)
                     /locus_tag="PKH10_13115"
     CDS             complement(226..>1675)
                     /locus_tag="PKH10_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29103.1"
                     /translation="FYVWADAGYDFVNEFIIYHHFKRVQGEIHKPNDLWTLYPRVLGY
                     ALFPWIAFVPMALYRFFHGDRDTGAFRLRLFLLSAFIGPFFFLAFSSTKFWHYIAPVV
                     PILAVMVGYYLTELWNAKWGLSRKLEVLTALFFVGIVARDIGDKFMVWMYMVTFYQER
                     KGPTLPAFIPVMAVVFILFGAVMLAAMFSERFKKWALGLIFVLPAAFMIYFFVDGMPK
                     IATWYSYKPLWDAYVKDSPERAPIAQYYKWLEKSLSFWSFNEITFLASDKEQAVLRFF
                     DRPGIQYAIVKSPDKNRFENIMKRVNKKTTVVAKIPQNFLYKVYGGDQKSFVNKDKYI
                     IQSVPDDAIKVGAVFDNAVELVGYRILQGDTTPKENELVRIEVYFKALRDNIPRDYTV
                     FVHVEGDQRDKRTKDDQPMAMGEYPTTFWKKGDIVKHPINVRIPTNNKNNYYWVLAGI
                     YQEDIRANISNPREVEHDGDNRLKLVKLDIER"
BASE COUNT          352 a          465 c          503 g          355 t
ORIGIN      
        1 tcgacggacc cgataatacg gtggtcgagg tcaacaccgt ggtcgacgag ccggtcgaga
       61 tagggcgccg ccgacaacgg cgtcgagaac tgtatcgtca gcatgtcgcc ctcccgcgaa
      121 agggtcgagg aaagaccggc gttctcgtga gactcgagaa ggtaatgaag caacacattg
      181 tccttcttat gcagtctcac caaccggtgc caactcacgc cgcccctagc gctcgatatc
      241 gagtttcacg agtttcaggc ggttgtcccc gtcgtgctcg acctcgcgcg gattgctgat
      301 gttggcgcgg atatcctcct gatagatgcc ggcgagcacc cagtagtagt tgttcttgtt
      361 gttggtcggg atgcgcacat tgatggggtg cttgacgata tcgcctttct tccagaaggt
      421 ggtcggatac tcccccatcg ccatcggctg gtcgtccttg gtccgcttgt cgcgctgatc
      481 gccttcgaca tggacgaaca ccgtgtagtc gcgggggatg ttgtcgcgga gcgccttgaa
      541 atagacctcg atacggacca gttcgttctc cttcggggtc gtgtcgccct gcaggatgcg
      601 atacccgacc agttcgacgg cgttatcgaa caccgcgccg accttgatgg cgtcatcggg
      661 cacgctctgg atgatgtatt tatccttgtt cacgaaactc ttctggtcgc cgccgtagac
      721 cttgtaaagg aaattctgcg gtatcttggc cacgaccgtg gtctttttgt tgacgcgctt
      781 catgatgttc tcgaaccggt tcttgtcggg actcttgacg atggcgtact gaatgccggg
      841 acggtcgaag aaacgcagga ccgcctgttc cttgtccgac gcgaggaagg ttatctcgtt
      901 gaacgaccag aacgaaagcg atttttcgag ccatttatag tactgggcta tcggcgcacg
      961 ttcggggctg tccttcacat aggcgtccca gagcggtttg taggaatacc aggtcgctat
     1021 cttgggcatg ccgtccacga agaaatatat catgaacgcg gcgggcagga cgaatatgag
     1081 cccgagcgcc cattttttga atctctcgct gaacatcgcg gcgagcatca ccgccccgaa
     1141 caggatgaat acgaccgcca tcaccggtat gaacgcgggc agcgtcggcc ccttgcgctc
     1201 ctgatagaag gtcaccatgt acatccagac catgaacttg tcgccgatgt cgcgcgccac
     1261 gatgccgacg aagaacagcg cggtcagcac ctcgagtttg cgcgacagcc cccatttcgc
     1321 gttccagagt tccgtcagat agtaaccgac catgacggcg agtatcggga ccaccggcgc
     1381 gatgtagtgc cagaacttgg tcgaagagaa ggcgaggaag aagaacggac cgatgaaggc
     1441 gctgaggagg aacagtcgca ggcggaacgc accggtgtcg cggtcgccgt ggaagaaacg
     1501 gtacagcgcc atcggcacga aggcgatcca cgggaagagg gcgtagccga gcacccgcgg
     1561 atagagcgtc cagaggtcgt tgggcttgtg tatctcgccc tgcacccgct tgaagtggtg
     1621 gtagattatg aattcattga cgaaatcgta gcccgcatcg gcccagacat agaag
//
LOCUS       DAOQUE010000570         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_97297_length_1670_cov_4.478638, whole genome shotgun sequence.
ACCESSION   DAOQUE010000570 DAOQUE010000000
VERSION     DAOQUE010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97297_length_1670_cov_4.478638"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PKH10_13120"
     CDS             <1..688
                     /locus_tag="PKH10_13120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT29104.1"
                     /translation="RITALADKNMATRQQADDAARRVEVARVTLDTAKKKLADTELKA
                     PFDAVVAKRLVNEGEYVRTGSAVFHLVQASPLRFRGEVPERYAPLVKSGIPVIVMPAS
                     APGTTAKGTIRRVGPLIAAENRSFPIEAEVPNGDLKIKPGTFAACRITLPGSDEVIAI
                     PEKALVTFAGTDKVFVFENGKIKERLVTLADRRAGKVLIAQGLSAGEQVVVSASESLY
                     DGMAAAPRAE"
     gene            693..>1670
                     /locus_tag="PKH10_13125"
     CDS             693..>1670
                     /locus_tag="PKH10_13125"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT29105.1"
                     /translation="MNALTIFIRRPVLATMLTVLLMVLGLFSYRAIGVDLTPKVEFPM
                     VTVTTVLRGASPEEVETLLTKPIEEAVNTVSGIDELSSTSSEGMSRVRVRFLLEKPTD
                     EAVQDVRDKVSAVVSLLPAGTEMPVVTRVDFDAFPVITFSVSGNRDPKELSEITRLSI
                     KESLENVAGVGAVTLSGYWKRAINIYLDAPKMQALGLSVASVKAALATQNVEVPAGRI
                     DKGEREEMLRVLGRVASIEELRRIAITDRNGRQITLGEIATVTDSVEEPRNLARLWKK
                     GDERAFAATISLDVVKQSGSNTVEVIAAVKERMAKIQPLLPKDIAVEVVS"
BASE COUNT          327 a          527 c          532 g          284 t
ORIGIN      
        1 ccgcatcacg gcgctcgccg acaagaacat ggcgacgcgc caacaggccg acgacgctgc
       61 gcgacgggtc gaggtagccc gcgtgacgct cgacaccgca aagaagaaac ttgccgacac
      121 cgaactcaaa gcgccttttg acgcggtggt cgccaagcgg ctggtcaacg agggggaata
      181 tgtccggacc gggagcgcgg tctttcatct ggtgcaggca tccccgctca gattccgcgg
      241 cgaggtacct gaacgctatg ctcctctggt aaaaagcggc attcccgtca tcgtgatgcc
      301 tgcgtcggct ccaggcacga ccgcgaaggg gaccatacgc cgcgtcggtc cgctcatcgc
      361 cgccgagaac cgatccttcc cgatagaggc ggaggtgccc aacggcgatc tcaagataaa
      421 accggggacc ttcgccgcct gccgcatcac cctgccgggc agcgacgaag tgatcgcgat
      481 ccccgaaaag gcgctcgtca ccttcgccgg caccgacaag gtcttcgttt tcgagaacgg
      541 gaagataaaa gagcgcctcg tgacgctcgc cgaccggcgc gccggcaagg tgctcatcgc
      601 gcagggactg tcggcgggcg aacaggtggt ggtgagcgct tcggaatcgc tttacgacgg
      661 catggcagcc gcgccgcgcg ctgagtgacc gcatgaacgc gctgaccatc ttcatccggc
      721 gaccggtgct ggccaccatg ctcaccgtgc tactcatggt gctcggcttg ttctcctacc
      781 gcgccattgg cgtcgacctc accccgaaag tggagttccc gatggtcacc gtgaccacgg
      841 tgctccgcgg cgcctcgccc gaggaagttg agacgctcct gaccaagccc attgaggagg
      901 cggtcaacac cgtgtcgggc atcgacgaac tttcctcgac cagcagcgag gggatgtcac
      961 gcgtccgggt ccggttcctg ctcgaaaagc cgacagacga ggcggtgcag gatgtccgcg
     1021 acaaggtctc cgcggtggtt tcgctcctgc ccgctggtac cgagatgccg gtggtcacgc
     1081 gggtcgattt cgacgccttc ccggtcatca ctttttccgt gtcaggcaac cgcgacccta
     1141 aggaactttc cgaaataacg cgtctttcga taaaggaatc gctcgagaat gtcgccgggg
     1201 tcggcgcggt tacgctctcc ggttactgga aacgggcgat caacatatat cttgacgccc
     1261 ccaagatgca ggcgcttggt ctgtcggtcg cgtcggtcaa agcggcgctc gcgacccaga
     1321 atgtcgaagt gccggcgggc cgcatcgata agggagagcg agaggagatg ctccgggtgc
     1381 tcggccgcgt cgcctcgatc gaggaattac gccgcatcgc gataaccgat cgcaacggtc
     1441 gccagataac gctgggcgag atcgcgacgg tgaccgattc ggtcgaggag ccgcgcaatc
     1501 tcgcgcgact ttggaagaag ggcgatgagc gcgccttcgc cgcgaccata tcgctcgatg
     1561 tggtcaagca gtcgggctcc aacaccgtcg aggtgatcgc ggcggtcaag gaacggatgg
     1621 cgaaaataca gccgcttctc ccgaaagaca tagcggtcga ggtggtctcc
//
LOCUS       DAOQUE010000571         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_97299_length_1670_cov_4.167183, whole genome shotgun sequence.
ACCESSION   DAOQUE010000571 DAOQUE010000000
VERSION     DAOQUE010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97299_length_1670_cov_4.167183"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PKH10_13130"
     CDS             complement(<1..327)
                     /locus_tag="PKH10_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29106.1"
                     /translation="MALVLSFLLGCSSNTPNSAECITAFDCEDGYTCQDGTCVALPDN
                     SVTGDGDTAGTDTSTDEMLDDTVTDEELPPDEEKADEATDTDEDTPIVTDDPIVTDED
                     TFLSDDD"
     gene            complement(469..1044)
                     /gene="rsxA"
                     /locus_tag="PKH10_13135"
     CDS             complement(469..1044)
                     /gene="rsxA"
                     /locus_tag="PKH10_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNT29107.1"
                     /translation="MTYFNIIIGSIFVNNIVLAMFLGICPFLGVSSKVSTSVGMGAAV
                     VFVMTLATLVTYLIQTLVLIPLSIGFMQTVVYILVIAALVQMVEIILKKISQPLYQAL
                     GIFLPLITTNCAVLGVALLVIKKNMPLADAMVYAVANAIGFTFALIVFAGIRERLALV
                     DIPKPMRGAPIALITAGLLSLAFMGFAGLVK"
     gene            complement(1055..1648)
                     /locus_tag="PKH10_13140"
     CDS             complement(1055..1648)
                     /locus_tag="PKH10_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNT29108.1"
                     /translation="MNNLQVFTRGLVKENPIFVLLLGMCPTLAVSSSAFNGLGMGLAA
                     TFVLVCSNVVIALLKNLIPDKVRIPAFIVVIASFVTIVDLSMQAFTPALSESLGVFIP
                     LIVVNCIILARAEAFASKNGVLGSLLDGLGMGLGFAIALTILGGIREILGDGKFFGIP
                     LFGESPTTILLFILPPGAFIALGLVIAFVNKFVKKTA"
BASE COUNT          392 a          448 c          531 g          299 t
ORIGIN      
        1 gtcgtcgtcg gagaggaagg tatcctcgtc ggtcacgatc ggatcgtcgg tgacgatcgg
       61 ggtatcttcg tcggtgtcgg tcgcctcgtc ggccttttcc tcgtcgggcg gcagttcttc
      121 gtcggtgacg gtgtcatcaa gcatctcgtc ggtggaggtg tccgtgccgg cggtgtcgcc
      181 gtcaccggtc acgctgttgt cggggagcgc cacacaggtg ccgtcctgac aggtatatcc
      241 gtcctcgcaa tcgaacgccg tgatgcactc ggcggagttc ggcgtgttcg aggaacaccc
      301 caaaagaaag gacaggacta acgccacgaa agaaatgatc gctctctcac gcgtcatttt
      361 aggggtcccc ccacgcacag tgaatgaaca tctgtagggt attttaaaaa aaagataagg
      421 aaaagcaagc actttttcgt tgaaaattcg ttgagaaaaa gaagggactt acttcacgag
      481 acccgcgaaa cccatgaagg cgagcgacaa gagtccggca gtgatgagcg cgatgggggc
      541 gccgcgcatc ggcttgggga tgtcgacgag cgccagccgt tcgcggatgc cggcgaaaac
      601 gataagagcg aaggtaaagc cgatcgcgtt ggcgaccgcg tagaccatcg cgtcggccag
      661 cggcatgttc ttcttgatga cgagcagggc gacgccgagc accgcgcagt tggtcgtgat
      721 aagcggcagg aagatgccga gcgcctgata gagcggctgg gatatctttt tcaggatgat
      781 ctcgaccatc tgcacgagcg ccgcgatgac gagaatgtag acgaccgtct gcatgaaccc
      841 gatggaaagc ggtatcaaca cgagtgtctg gatgagataa gtaacgagcg tcgcgagcgt
      901 catgacgaag acgaccgccg cgcccatccc gaccgaggtc gacaccttgc tcgatactcc
      961 gagaaagggg cagatgccca ggaacatcgc cagtacgatg ttgttgacga atatcgagcc
     1021 gatgatgatg ttgaagtatg tcatggtcta ccccctacgc cgttttcttg acgaacttgt
     1081 tcacgaaggc gatcacgagc ccgagggcga tgaaggcgcc gggcggcagg atgaagagca
     1141 ggatggtcgt cggactctcg ccgaacagcg ggatgccgaa gaatttgccg tcccccagta
     1201 tctcgcgaat accgccgagg atcgtaagag cgatcgcgaa gccgagcccc atgccgagac
     1261 cgtccagcag actgcccagc acgccgttct tcgaggcgaa ggcctcggca cgggcgagaa
     1321 tgatgcagtt gacgacgata agcgggatga agacgccgag cgactcggag agcgccgggg
     1381 tgaaggcctg catcgaaagg tcgacgatcg tcacgaacga ggcgatgacc acgataaagg
     1441 cggggatgcg gaccttgtcg gggatgaggt tcttcaagag cgcgatgacg acattggagc
     1501 acacgaggac gaaggtcgcc gcgagaccca tgccaagccc gttaaaggcc gaactcgaca
     1561 ccgcaagcgt cggacacatg ccgagcagga gaacgaagat cggattctcc ttgaccagcc
     1621 cccgggtgaa tacctgcagg ttattcattt ctcacctccc ttcttgaagg
//
LOCUS       DAOQUE010000572         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_99293_length_1650_cov_3.645141, whole genome shotgun sequence.
ACCESSION   DAOQUE010000572 DAOQUE010000000
VERSION     DAOQUE010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99293_length_1650_cov_3.645141"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PKH10_13145"
     CDS             <1..906
                     /locus_tag="PKH10_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29109.1"
                     /translation="WLWHDIVARFKEYVLFHVNHYGISMYYLGEVYSTPRPPWSAPFV
                     MMLVTTPLLTVLCAVLGFLLYRWSDDRDRRRPMLLVSLSAFVAIGTVMFLPAPFYSGV
                     KLFQPFFPFLAVAAGVGLWRVLDRATILPERWRFAPFGVVFIPVILSMVDLRHDHLSY
                     YSELIGGTKGAAQYKFETHYYDLFYYDLAEYFNRELKTFSPVAFDPNGKEYPFTSGYL
                     KRAKLLTPKFYYSDNKDAAAYYVLTHEYRWREYPDLLRKVKWRTPVYVLERQGVPLLT
                     VYGREKASTPPPPHEPTDLPGADGD"
     gene            complement(922..>1650)
                     /locus_tag="PKH10_13150"
     CDS             complement(922..>1650)
                     /locus_tag="PKH10_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT29110.1"
                     /translation="VGRVFTGLVYDLPAADDEPEPVAPPPPAPEPKPEPAPAPPPPPP
                     APEPPKDTDKDGLLDPDDKCPDEPEDKDQFEDTDGCPDPDNDKDTVLDEKDSCPNEEE
                     TLNGFMDEDGCPDKAPEKELEKIVVTEKEIEIKDQVLFALNRADISSKSFAMLDEIAS
                     VLIANPKIKIKIEGHTDNLGKPASNKKLSQDRADSVRSYLIRKGVAADRLTAEGFGQE
                     RPIADNATKTGREANRRVEFHIDK"
BASE COUNT          256 a          506 c          498 g          390 t
ORIGIN      
        1 tggctgtggc acgatatcgt cgcgcggttc aaggagtatg tcctgttcca tgtcaaccac
       61 tacggcatca gcatgtacta tctcggcgag gtctactcga cgccgcgacc gccgtggtcg
      121 gcccccttcg tgatgatgct ggtgaccact ccgctcctca cggtgctatg cgccgtactc
      181 ggctttctgc tgtaccgctg gagcgatgat cgcgaccggc gccgaccgat gcttctcgtg
      241 tcgttgtcgg ccttcgtcgc gatcggcacg gtgatgttcc tgccggcgcc tttctacagc
      301 ggcgtgaaac tgttccagcc cttcttcccg ttcctcgcgg tcgccgcggg cgtcggactc
      361 tggcgggtgc tcgatcgggc gacgatcctg ccggagcggt ggcggttcgc cccgttcggc
      421 gtcgtgttca taccggtcat cctttcgatg gtggacctca ggcacgatca cctgtcgtat
      481 tacagcgaac ttatcggtgg gaccaagggg gcggcgcagt ataagttcga aacccactac
      541 tacgacctgt tctactacga ccttgccgag tatttcaatc gcgaactgaa gaccttctcg
      601 cccgtcgcgt tcgacccgaa cggcaaggaa tatcccttca cctccggcta tctcaagcgg
      661 gcgaaactgc tgacaccgaa attctactat tccgacaaca aggatgcggc cgcctactat
      721 gtgctgaccc acgagtaccg ctggcgcgag tacccggatc ttttgcgcaa ggtcaagtgg
      781 cgtacaccgg tctacgtcct cgaacgacag ggggtgccgc ttctcaccgt gtatggaagg
      841 gagaaggcgt ccaccccacc tccgccccac gaaccgaccg atctgcccgg agcggacggc
      901 gactgatcac taacgatccc gttacttgtc gatgtggaat tcgacgcggc gattggcctc
      961 gcggcccgtt ttggtcgcgt tgtcggcgat gggccgctcc tgtccgaacc cttcggcggt
     1021 gagccggtcg gcggcgacac ccttcctgat aaggtagctt ctgaccgaat cggcgcgatc
     1081 ctgcgagagt ttcttgttgc ttgccggttt gccgagatta tcggtgtgtc cttcgatctt
     1141 gatctttatc ttcggattgg cgatgagcac cgaggctatt tcgtcgagca tcgcgaacga
     1201 ctttgaggaa atgtcggccc tgttgagcgc gaagagcacc tgatccttta tctctatctc
     1261 cttctcggtc acgacgatct tctcgagttc cttttcgggt gccttgtcgg gacagccgtc
     1321 ctcatccatg aaaccgttca gagtttcttc ctcattcgga caggaatcct tttcatcgag
     1381 caccgtgtcc ttgtcgttgt cgggatcggg acacccatcg gtgtcctcga actggtcctt
     1441 gtcctccggc tcatccgggc acttgtcgtc ggggtcgagc aggccgtcct tgtcggtatc
     1501 tttcggcggt tcaggcgcgg gtgggggcgg tggcggcgca ggcgccggtt cgggtttcgg
     1561 ttcgggtgcg ggtggtgggg gggcgaccgg ttccggctca tcgtcggcgg ccggcagatc
     1621 gtagacgagc ccggtgaaga cgcggcccac
//
LOCUS       DAOQUE010000573         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_99385_length_1649_cov_3.932246, whole genome shotgun sequence.
ACCESSION   DAOQUE010000573 DAOQUE010000000
VERSION     DAOQUE010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99385_length_1649_cov_3.932246"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKH10_13155"
     CDS             complement(<1..369)
                     /locus_tag="PKH10_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29111.1"
                     /translation="MRYAIMPIILLIAMLLIAACDEPSPQWRDDNAALDEETGEKDTG
                     DENDPGDGGLIGEEDPMPDDDQPTIDCTPATPTTNEENALRQLFPNGEIFCAAKTGSG
                     AGYFIAIKGGTTVGYAFPASN"
     gene            complement(366..>1649)
                     /locus_tag="PKH10_13160"
     CDS             complement(366..>1649)
                     /locus_tag="PKH10_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29112.1"
                     /translation="DLRRKCSTTTTPVIVAPPTPAPTAVPAQPSSNAAPATVAVQKRP
                     SSFENEDDGFAPFLAYTTLWSAGYGLMMPYVFGGDNAHWAYYPGTSLLAGGAAFTLGY
                     VLGRNADITRGMALGSAEGALRGAVDGASLFWLIAGSIELESDSDGWRNDIGLQPDTY
                     TRLLLASTMLFSMAEYAVGMWYANHYGLTGGEARMLGVGSTLGYGIMMELALTVFMGL
                     DSFALPAIRVMPAMLLAGGAGGLFLGHFANRWDHYTEGDASLVQSFSAITSFFPPSIA
                     LAAKTDDARVYPGLFLAGTISGAVGGYFLLKGLDFSNMDAFIIGLGTAAGGLTTTGIA
                     FLATMETKQYQTLGVAASLGTLAGFGLMYYVYHDRGLRQAQEREVSSWNLQLNPAGMV
                     TVLSGGRSVAPRTEEDLRILRETPPSPIVSFEYHW"
BASE COUNT          282 a          490 c          572 g          305 t
ORIGIN      
        1 gttgctggcg gggaaggcgt agccgaccgt cgttccgcct ttgatcgcga tgaaatagcc
       61 ggcgccgctt ccggtcttcg ccgcgcagaa tatctcgccg ttcgggaaca gttgccggag
      121 cgcgttctcc tcgttcgtcg tcggtgtcgc gggagtgcag tcgatcgtcg gttggtcgtc
      181 gtcgggcatc ggatcttctt ccccgatcag tccaccgtcc ccggggtcgt tttcgtcccc
      241 tgtgtccttt tctcccgttt cctcatccag tgctgcgtta tcatccctcc actgtggcga
      301 cggttcgtcg caggcggcga tcagaagcat ggcgatgagc aggatgatgg gcatgatcgc
      361 gtatctcacc agtggtattc gaacgacacg atcggcgacg gtggcgtctc gcgcagtatg
      421 cgaagatcct cttcggtgcg cggtgcgacg gatcgtccgc ccgacagcac cgtgaccatc
      481 ccggccgggt tcaactgaag gttccaactg ctcacttccc gttcctgtgc ctgccgcagg
      541 ccgcggtcat ggtagacata gtacatgagc ccgaagccgg caagcgtgcc gagcgaggcg
      601 gcgacgccga gcgtctggta ctgttttgtt tccattgtcg cgaggaaggc gatgccggtg
      661 gtggtgagac cccccgcggc ggtgccgagc ccgatgatga aggcgtccat gttagaaaaa
      721 tcgagccctt tgaggaggaa ataaccgccg accgcgccgc tgatggtgcc ggcaaggaag
      781 agccccggat agacccgggc gtcgtcggtc ttggcggcga gagcgatgga tggcggaaag
      841 aaactggtga tggcgctgaa ggactgtacg agcgatgcgt cgccttcggt gtaatggtcc
      901 caccggttcg cgaagtggcc gaggaacagc ccgccggccc cgcccgcaag gagcatggcg
      961 ggcatgacgc gaatggccgg cagggcgaac gagtcgagcc ccatgaatac ggtcagggcg
     1021 agttccatca tgataccgta cccgagcgtc gagccgacac cgagcatccg tgcctcaccg
     1081 ccggtgagtc cgtaatggtt cgcgtaccac atgccgacgg cgtattcggc catgctgaag
     1141 agcatagtcg atgcgagcag aagccgcgtg taggtgtcgg gttggagccc gatgtcgttg
     1201 cgccacccgt cagagtcgga ttccaattcg atgctgccgg cgatgagcca gaagagggag
     1261 gcgccatcca ccgcgccgcg gagcgcccct tcggccgagc cgagcgccat gccgcgggtg
     1321 atgtcggcgt tcctccccag cacatagccg agcgtgaagg cggcgccgcc ggcaaggagc
     1381 gacgtgccgg gatagtaggc ccaatgggcg ttgtcgccgc cgaagacgta gggcatcatg
     1441 agtccgtaac cggcgctcca gagagtggtg taggcgagga acggggcgaa gccgtcatcc
     1501 tcgttctcga aggaggatgg ccgtttctgg accgcgacgg tcgcgggtgc ggcgttcgat
     1561 gagggctgag ccgggaccgc cgtcggcgcc ggtgtcggtg gcgctacaat gacgggcgtt
     1621 gtcgtggtcg agcatttccg gcgcagatc
//
LOCUS       DAOQUE010000574         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_101116_length_1633_cov_3.112167, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000574 DAOQUE010000000
VERSION     DAOQUE010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101116_length_1633_cov_3.112167"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKH10_13165"
     CDS             complement(<1..264)
                     /locus_tag="PKH10_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29113.1"
                     /translation="MSSRTVPVTIMAIFAVAIWFVVSSCSLEETAGYIAGDYCVEEGM
                     KVCDDTFTVVLLCVSDGASLFWQPDEECAKGCTMDSGIGACIDA"
     gene            445..882
                     /locus_tag="PKH10_13170"
     CDS             445..882
                     /locus_tag="PKH10_13170"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNT29114.1"
                     /translation="MKVWLTHRDGVSFETGSDLPVTIRMSDPARLSAGEACGETPKHL
                     FLQSVAGCTGIDVVMVLDKMRVPVPQGFRIEVEAPLTEEHPKVFSSFSLTYLFEGAVE
                     PDKLVRAVKLSQEKYCGISAMFRRIAPLSWRVLLNGVEIARGA"
     gene            complement(896..>1633)
                     /locus_tag="PKH10_13175"
     CDS             complement(896..>1633)
                     /locus_tag="PKH10_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29115.1"
                     /translation="YQKMYLPTHAANTCILTLATAIHLGIPKRRLTTIGIAAYLHDIG
                     IPDEDQRDMRNVHTELGFAYLSRLNTLNFSMMESAITAAMHHDTYDFRGLIAPPPDKP
                     APPTPITELIKVCDYYDIATRWWPWSPGKPLSRVNAMEAIFKATAEKKAFSATAARAL
                     FAVIGIYPPGQVLRVNKKRSLACSLGGFFTSYDENRLILLSDQMDFKGYTTLNAAALS
                     HLPAEQHHRIPPRTYKEIFQSFQVTVT"
BASE COUNT          319 a          435 c          554 g          325 t
ORIGIN      
        1 ggcgtcgatg caggcgccga tgccgctgtc catggtacac cccttggcgc attcctcgtc
       61 cggttgccag aaaagcgagg cgccgtcgga aacgcacaaa aggacgaccg taaaagtgtc
      121 gtcacagacc ttcatccctt cctcgacgca gtagtcgccg gcgatatacc cggcggtctc
      181 ctcgagagag caggaagaga cgacgaacca gatcgcaacg gcgaagatcg ccatgatcgt
      241 gacgggaaca gtgcgcgaac tcatgccggc ctcccataca gaaatggaca ggaaccccaa
      301 aggaccgaac cgatgttagt gcgatatcga acgattgtca agagaggcca cgctcaacca
      361 tcggtccgtc aggagttttt cacttgacta ccgttggtgt agcggatata tgatccggga
      421 aggaaggcat gatggagggt agtgatgaaa gtgtggttga cgcatcggga cggcgtttcc
      481 ttcgagaccg gcagcgacct gccggtgacg atccgcatga gtgaccctgc gcggttgtcg
      541 gcgggcgagg cctgcggcga gacgcccaaa cacctgttcc tgcagtcggt tgccggatgt
      601 accggcatcg atgtggtgat ggtgctcgac aagatgaggg tgccggtccc gcaagggttc
      661 cgcatcgagg tcgaggcgcc gctcaccgaa gagcatccga aggtgttctc ttccttttcg
      721 ctgacctatc tgttcgaagg cgctgtcgag ccggacaaac tggtccgtgc cgtcaaactg
      781 tcgcaggaga agtattgcgg catatcggcc atgttccgca ggatagcgcc gctctcatgg
      841 cgggtgctgc ttaacggcgt cgagatagcg cggggcgcat aagcggcccc ctatatcagg
      901 tcacggtcac ctggaaactc tggaatatct ccttgtaggt ccgtggcggg atgcggtggt
      961 gctgttccgc cgggagatgg gacagcgcgg cggcgttgag tgtcgtgtag cccttgaaat
     1021 ccatctggtc ggacaacagt atcaaccggt tctcgtcgta actggtgaaa aagcctccca
     1081 gcgaacaggc gagcgaccgc tttttgttga cgcgaaggac ctggcccgga ggatagatgc
     1141 cgatcacggc gaacagggcg cgcgcggcgg tcgccgaaaa cgcctttttc tcggcggtcg
     1201 ccttgaatat cgcctccata gcgttgacgc gggagagcgg tttgccggga ctccacggcc
     1261 accagcgggt cgcgatgtcg tagtagtcgc agaccttgat gagttcggtg atgggggtcg
     1321 gcggcgccgg cttatcggga ggcggcgcga tgagcccgcg gaagtcgtag gtgtcgtggt
     1381 gcatcgcggc ggtgatggcc gattccatca tcgagaagtt cagcgtattg agccggctca
     1441 gataggcgaa gccgagttca gtgtggacat tccgcatgtc gcgctgatcc tcgtcgggta
     1501 tgccgatgtc atggaggtag gcggcgatgc cgatcgtggt cagtcgccgt ttgggtatgc
     1561 cgaggtggat ggcggtcgcg agggtgagta tgcaggtgtt ggccgcgtgg gtcggcaggt
     1621 acatcttctg gta
//
LOCUS       DAOQUE010000575         1632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_101215_length_1632_cov_4.082435, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000575 DAOQUE010000000
VERSION     DAOQUE010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101215_length_1632_cov_4.082435"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..>1632
                     /locus_tag="PKH10_13180"
     CDS             42..>1632
                     /locus_tag="PKH10_13180"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNT29116.1"
                     /translation="MEKFRDAGFNTVDIYVAWNRIEPREGEFVFNDPDITGFLDLCTE
                     LGLMVYFRPGPYICNELDGGGLPAWLFTKTTKKNKNDDDRPNLRTDDPDYLFYATRYL
                     TELNKKILPYLASNGGPVVLYGIENEYNWFETFFEVDKLFAYDGGTERGLTQATGVKK
                     MFESLRDAVIASGVDVPITTCPGDATVSGMGDVTGVIPMPNIYKNETTERYAYDTLIS
                     MHDPTKFGGAYTNYPSGSTETDRSPGRMMRLFLGGMDGYFGFNIAGFHQEGRQNALTL
                     DNSGLETIVDLSPQNVLSLFVSPTVNYFSGVIDFYGPIGAAGTLREKYFEFRRFNMFL
                     DDVEPLLAGLLLPAVVTTGPCADCPVAVLNGSLGALEGQTRRHYYFTAADGAIFLGLT
                     NESDAPITVAPGEITVGERSIPRFVPLTVPVGVNPGAPEDPHGDTSYARILIAGLPIG
                     PVRIAHTTSEVLRLRRFNDEVLVALHGPKGSEGELLFDDLPDGAKVTYRDGAVTQRDG
                     ETGTIALTYTHDGIAQALLSAG"
BASE COUNT          327 a          552 c          470 g          283 t
ORIGIN      
        1 tccagtggtt ccgacttccg ccttcggcct ggcgcgaccg tttggaaaaa ttccgtgacg
       61 ccgggttcaa caccgtcgat atctatgtcg cgtggaaccg cattgaaccg agggaaggag
      121 agttcgtgtt caacgatccc gacatcaccg gctttctcga cctgtgcacc gaactcgggc
      181 tcatggtcta tttccgtccc ggcccctaca tctgcaacga actcgacggc ggcggcctgc
      241 ccgcatggct gttcacaaaa accaccaaga agaacaaaaa tgacgatgac cgccccaatc
      301 tgcggaccga cgatcccgac tatctcttct acgcgacccg ctacctgacc gaactgaaca
      361 agaagatact tccctacctc gcctcgaacg gcggcccggt ggtcctatac ggcatcgaga
      421 acgaatacaa ttggttcgag accttcttcg aggtggacaa attgttcgcc tacgatggcg
      481 gcaccgagcg aggactcacg caggcgaccg gcgtgaaaaa gatgttcgaa tcgctccgcg
      541 acgcggtcat cgccagcggg gtggacgtcc cgataacgac ctgccccggc gacgcgacgg
      601 tgtcgggaat gggcgatgtc accggcgtca tcccgatgcc gaacatctac aagaacgaaa
      661 cgaccgagag atatgcctac gacaccctca tatcaatgca tgacccgacg aagttcggcg
      721 gtgcctacac gaactatccg agcgggtcga ccgagaccga ccggagcccg ggccggatga
      781 tgcggctctt cctcggcggg atggacggtt acttcggctt caacatcgcc ggcttccatc
      841 aggagggacg gcagaacgcc ctcacgctcg acaacagcgg gctcgagacc atcgtggacc
      901 tatcgcccca gaatgtgctt tcgctcttcg tttcgcccac cgtcaactac ttctcgggcg
      961 tcatcgattt ctacggaccc atcggcgccg ccggcacgct gcgcgagaaa tatttcgagt
     1021 tccgccgatt caacatgttc ctcgacgacg tcgagccgct cctcgcgggg cttctgcttc
     1081 ccgcggtcgt gacgaccggc ccctgcgccg actgtcccgt cgcggtactg aacggctctc
     1141 tgggcgcgct ggaaggccag acgcgccggc actactactt cactgccgcc gacggcgcga
     1201 tcttcctcgg gctcaccaac gaaagcgacg cgccgataac ggtcgccccc ggcgagataa
     1261 cggtcgggga acggagcata ccgcgcttcg ttccgctgac ggtgccggtc ggcgtcaatc
     1321 ccggcgcccc tgaggatcct cacggggaca ccagttatgc ccggatactc atcgccggtc
     1381 ttccgatcgg accggtacgc atcgcccata cgaccagcga ggtcctgcgc ctgcgtcgtt
     1441 tcaacgacga agtactcgtc gccctccacg ggccgaaggg atcggagggg gaactgctgt
     1501 tcgacgacct gccggacggc gcgaaagtga cctaccgcga cggagccgtg acccagcggg
     1561 acggagagac cgggaccatc gccctcacct acacacacga cggcatcgcg caggcgctcc
     1621 tctcggcggg cg
//
LOCUS       DAOQUE010000576         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_103448_length_1611_cov_3.416452, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000576 DAOQUE010000000
VERSION     DAOQUE010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103448_length_1611_cov_3.416452"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /gene="mazG"
                     /locus_tag="PKH10_13185"
     CDS             complement(<1..544)
                     /gene="mazG"
                     /locus_tag="PKH10_13185"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNT29117.1"
                     /translation="MTEQRGIERLKAIMATLRGECGCPWDRKQTIPSLKPYVIEEAYE
                     VVEAMDEGPDELQDELGDLLLQVVFIAQMAQEEGLFDFDSVAERISDKLVRRHPHVFG
                     TTQVKDADEVVQNWNQIKKDKEAKKYLMDGIPTAMPATLLTQRYADRAASVGFDWGRW
                     EDTLAKIAEETAELKEAVASG"
     gene            complement(554..>1611)
                     /locus_tag="PKH10_13190"
     CDS             complement(554..>1611)
                     /locus_tag="PKH10_13190"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNT29118.1"
                     /translation="VFTSNNAGELIARLIAHRHAVEGLTIEEAITRAQSDLKGAFSAL
                     LIHRDRMYAFRDPHGFRPYVMGHLTWQGTGEMPAEGTCFASESCAFGIIGARFIRELK
                     PGEILKLERNRPVTTVAVNGLRPRHCVFELIYFSRPDTLTFGEFVHDVRKKIGATLAR
                     GDRDIPPGDDLVVMAVPDSSNFVALGYAAEKKAPFDMGLIRNHYVGRTFIRPHQEKRD
                     EGVKQKFNPLPGFFPGKRVILVDDSIVRGTTLRKIVRMIKRNGAKEVHVRIGSPRVTG
                     SCFYGIDTPTRTELIANNKSIAETAEYLTADSLRHIALPDLLSLLARPDDFCNACFSG
                     DYIYVPDSFVKTNAPGC"
BASE COUNT          255 a          483 c          555 g          318 t
ORIGIN      
        1 cgcccgaggc gaccgcttcc ttgagttcgg ccgtttcttc cgctatcttg gcgagcgtgt
       61 cctcccacct gccccagtcg aacccgaccg acgcggcgcg gtcggcgtag cgctgggtga
      121 ggagcgtcgc gggcatcgcg gtggggatgc cgtccatgag atatttcttc gcctctttgt
      181 ccttctttat ctggttccag ttctgcacca cctcgtcggc gtccttcacc tgcgtcgtgc
      241 cgaacacatg gggatggcgc cgcaccagtt tgtcggagat acgctccgcg accgaatcga
      301 agtcgaagag tccctcctcc tgcgccatct gtgcgatgaa cacgacctgc aagaggagat
      361 cgcccagttc atcctgcagt tcatcgggtc cctcgtccat cgcctcgacc acctcgtagg
      421 cctcctcgat gacataaggt ttgagcgacg ggatggtctg cttgcggtcc cacggacagc
      481 cgcactcgcc gcgcagggtc gccatgatgg ccttgagccg ttcgatgccg cgttgttcgg
      541 tcatggggcc gcgtcaacag ccgggggcgt tggtcttgac gaacgagtcg gggacataga
      601 tgtagtcgcc gctgaagcag gcgttgcaga aatcgtcggg ccgcgcaagg agcgacagca
      661 ggtcggggag cgcgatgtgg cgcagggagt cggcggtgag atattcggcg gtctccgcta
      721 tcgacttatt gttcgcgatg agttcggtgc gggtcggcgt gtcgatgccg tagaagcacg
      781 agccggtgac gcgcggcgag ccgatgcgga catggacctc ctttgcgccg ttcctcttga
      841 tcatgcggac gatcttgcgg agcgtcgtgc cgcgcacgat cgagtcgtcg acgagtatca
      901 cccgcttgcc ggggaagaag ccggggagcg ggttgaactt ctgtttgacc ccttcgtcgc
      961 gcttctcctg atgggggcgg atgaaggtgc ggccgacata atgattgcgg atgagcccca
     1021 tgtcgaacgg ggccttcttc tcggccgcgt agccgagcgc tacgaagtta gacgagtcgg
     1081 ggaccgccat gaccacgaga tcgtcgccgg gcgggatgtc gcggtcgccg cgggcgagcg
     1141 tcgcgccgat cttcttgcgg acatcgtgga cgaactcgcc gaaggtgagc gtgtcggggc
     1201 gcgagaagta gatgagttcg aacacgcagt gacggggccg caggccgttc accgcgacgg
     1261 tcgtgacggg acggttgcgc tcgagtttca gtatctcgcc cggcttcagt tcgcggatga
     1321 agcgggcgcc gatgatgccg aaggcgcacg attccgaggc gaagcaggtc ccctcggccg
     1381 gcatctcgcc cgttccctgc caggtcaggt ggcccatgac atagggccgg aatccgtgcg
     1441 gatcgcggaa ggcgtacatc cggtctcggt ggatgaggag cgccgaaaag gctcccttca
     1501 ggtcgctctg cgcccgggtt atggcctcct cgatggtcag cccctcgacg gcgtggcggt
     1561 gggcgatgag ccgcgcgatg agttccccgg cgttgttgct cgtgaagacg a
//
LOCUS       DAOQUE010000577         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_103763_length_1608_cov_4.804250, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000577 DAOQUE010000000
VERSION     DAOQUE010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103763_length_1608_cov_4.804250"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1608
                     /locus_tag="PKH10_13195"
     CDS             <1..>1608
                     /locus_tag="PKH10_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNT29119.1"
                     /translation="PDSAMTNVLENPIVIFSRPIYRDSVSHDTLYLIQNGEVNVSLTA
                     MQSAQPTSLMVMPMPALIASKTIPAHLYVTEGVWDYRGNPVIATMRTWDEIGGAPATS
                     AITPNVVLGVTPASASTVKGGQHFAINWPAGGENDKFIMAPASINDDSIYLDQINKNG
                     TDGSVTASSTTFTVGTAGTFAAGDVGKVIETSGSGTAGNNGRRTILSVDSDTIVTLDA
                     PFAADETDLAWALIDQIPFELYFVPEQAGNVAEIYPGRFIRHDAGAVRLTIRKDRIAN
                     LYTIASTDSDMVLNYTVETTAPAVNASYGEDGNGWVALSDATGLAARTQLVVAFNEPV
                     DPASFVMGGTVTLTNTGGTAIGATISVVDTMVVIKPNAPLAVAGNPYALTFDGVTDTA
                     GNALAAPYTVSFSIEETAPTILSVSPDNSTSEVSVDTSVRVRASEPMSISTITRSTVT
                     TDGIFRVTRDTPPACGADAEDDVFGCLALDSSSRRVTFVPLPNDLHDETAYTLTIAST
                     VSDLAGNTLGTDFTSTFDTIDGDDVGP"
BASE COUNT          330 a          552 c          441 g          285 t
ORIGIN      
        1 tcccgattcg gcgatgacca atgtcctcga gaacccgatc gtcatcttct cgcggcccat
       61 ttaccgcgac agcgtttcgc acgacacgct ctatctgata cagaacggag aggtcaatgt
      121 gtccctgacc gcgatgcagt cggcccaacc gaccagtctg atggtgatgc cgatgccggc
      181 gctgattgcc agcaagacca ttcccgcgca cctctatgtc actgaagggg tctgggacta
      241 ccgcggcaac ccggtcatcg ccaccatgag aacatgggat gagatcggcg gcgctccggc
      301 aacaagcgcc atcaccccga atgtcgtact cggtgtaaca ccggcaagcg cctcgacggt
      361 gaagggcggc caacacttcg ccatcaactg gcccgccggc ggcgagaacg acaaattcat
      421 catggccccg gcgagcatca acgacgactc gatctatctc gaccagatca acaagaacgg
      481 caccgacgga tcggtcaccg cctccagtac caccttcacc gttggaacgg cggggacctt
      541 cgccgccggt gatgttggta aagtgataga gaccagtggt tctggcacgg cgggcaacaa
      601 cgggcgccgc accatcctct cggttgacag cgacaccatc gtcacgctgg acgccccctt
      661 cgccgctgac gagaccgacc ttgcttgggc gcttatcgac cagataccgt tcgaactcta
      721 cttcgtgcct gaacaggccg gtaatgtcgc cgagatatat ccgggacgct tcatccgaca
      781 cgatgccgga gcggtgcgac tgaccattcg caaggatcgg atagcgaacc tctataccat
      841 cgcctcgacc gattccgaca tggtgctgaa ttacaccgtc gaaacgaccg cccccgcagt
      901 gaacgcatcg tacggcgagg acgggaacgg ctgggttgcg ctttcggacg ctaccggtct
      961 tgcggcccgg acgcaactgg tcgtcgcctt caatgaaccg gtcgatcccg cctccttcgt
     1021 gatgggaggc accgtgacgc tcaccaatac cggcggaaca gcgatcggag ccacgatcag
     1081 tgtggtcgat acgatggtcg tcatcaaacc gaacgcgcca cttgccgtag cgggcaaccc
     1141 ctacgcgctg accttcgacg gtgtcaccga caccgcaggg aatgcgctgg cagcacccta
     1201 taccgtctcc ttctcgatcg aagagacggc gccgaccatc ctgtcggtca gtcccgacaa
     1261 ctccacctcc gaggtcagtg tggacacatc ggtgcgcgtt cgcgccagtg aaccgatgag
     1321 catcagcacc atcacccgga gcaccgtcac gaccgacggc atcttccggg tgacccgcga
     1381 cacgccaccc gcctgcggcg ccgatgccga ggacgatgtg ttcggctgtc tcgccctcga
     1441 ctcctcttcc cgtcgcgtca cctttgtacc gcttccgaat gatctgcacg acgagaccgc
     1501 ctatacgctc accatcgcct cgaccgtttc cgatctggcg ggcaacacgc tcggcaccga
     1561 cttcacgagc accttcgaca ccatcgacgg tgacgatgtc ggtcccat
//
LOCUS       DAOQUE010000578         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_104084_length_1605_cov_3.958065, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000578 DAOQUE010000000
VERSION     DAOQUE010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104084_length_1605_cov_3.958065"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PKH10_13200"
     CDS             complement(<1..348)
                     /locus_tag="PKH10_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29120.1"
                     /translation="MFAGTRLKTALIVGSLLAGIIPLMTLTFAGTRIFGEELRAQAFS
                     QLESVRDLKKNRIEETLRLLPSLSSNEIRRIMAERSGMGETGETYLVGPDRTPLSEPY
                     LHGDPAASKGSLKI"
     gene            complement(359..1054)
                     /locus_tag="PKH10_13205"
     CDS             complement(359..1054)
                     /locus_tag="PKH10_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT29121.1"
                     /translation="MDPIMNSRVLFVEDEENLLNSISFILENEGFSVHRCRSGEEALA
                     SVEGIEPDLVLLDIVLPGMDGFEVAERLRTNKATERICIIMLTGRAIEEEIISALERA
                     ADDYIVKPVRPRMLIARINAVLRRKTAQAPEMTVLSFDSLTVNTTSRQADIAGEPLSL
                     TKTEFDILALLARAPDTVFSRKQIIEAIRGEDYFVTDRSIDFQIFGLRKKLGPHAHRL
                     ITIRGIGYQFRSS"
     gene            1214..>1605
                     /locus_tag="PKH10_13210"
     CDS             1214..>1605
                     /locus_tag="PKH10_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HNT29122.1"
                     /translation="MAQNETAKNGNIFFTDGKLLGRDLKPDFEKAIEYLLVKDRTTVN
                     SRDIYFALAFAIRHRLIRKWLRTQYEYNKQGAKRVYYLSMEFLMGRLLGNILNNMAYY
                     QECSDILMQLGYNLEDIRELEPDMGLGNG"
BASE COUNT          358 a          419 c          494 g          334 t
ORIGIN      
        1 tatcttgaga gagcctttcg aggcggccgg gtcgccatga agatagggct cgcttagcgg
       61 ggtgcggtcc ggacccacca gatatgtctc gcctgtctct cccatcccgg agcgctcggc
      121 catgatgcgg cgtatctcat ttgatgagag agagggaaga aggcgcaacg tttcctcgat
      181 acggtttttt ttgagatcgc gcaccgattc gagttgactg aaggcctgcg cccggagttc
      241 ctcgccgaat atgcgggtgc ccgcgaaggt cagcgtcatg agagggatga tgcctgcgag
      301 tagcgaaccg acgatcagag ccgttttcag gcgggtgccg gcgaacatgg gcgccgggtc
      361 aggaagaacg gaactggtag ccgatcccgc gtatcgtgat gagccgatga gcgtgggggc
      421 cgagtttctt gcgcaggccg aatatctgga agtctatgga acgatcggtg acgaaataat
      481 cctcgccgcg tatcgcctcg atgatctgtt tgcgcgagaa cacggtgtcc ggcgcacgag
      541 cgagcagggc gaggatgtcg aactcggtct tggtgaggga gagcggctcg ccggcgatat
      601 cggcctgacg tgaggtggtg ttcacggtca gggaatcgaa agaaaggacg gtcatctcgg
      661 gggcctgtgc ggtcttgcgg cggaggacgg cgttgatgcg cgcgatgagc atccggggac
      721 ggaccggttt gacgatgtag tcgtcggccg cccgctcgag cgccgatatt atctcctcct
      781 cgatcgcgcg tccggtcaac atgattatgc atattcgttc ggtcgctttg ttggtgcgca
      841 accgttcggc cacctcgaag ccgtccatgc cggggagcac gatatcgaga agtaccagat
      901 cgggctcgat gccctcgacc gacgcgagtg cctcctctcc cgaccggcac cggtggaccg
      961 aaaacccttc gttctcgagg atgaatgaga tactattaag aagattttcc tcatcctcga
     1021 cgaaaagcac ccggctgttc atgatggggt ccatcgtttc cttgctccca caatgacgca
     1081 agcatatagc aaacttccgt acatttacaa aaaaaatact aaaaaagaag agggaggacg
     1141 cattaaaaat ggaaaaaggt gatgggatgg ctatgatacc ggcgttccat cacgtggcga
     1201 gacggaggat gttatggccc agaacgagac ggcgaagaac gggaacatct ttttcaccga
     1261 cgggaaactg ctggggcgcg acctcaaacc cgacttcgag aaggcgatag aatacctgct
     1321 cgtcaaggac cgcacgaccg tcaattcgcg tgacatctat ttcgcgcttg ccttcgccat
     1381 acggcaccgg ctcatccgta aatggcttcg cacgcagtac gagtacaaca aacagggagc
     1441 caagcgggtg tattaccttt ccatggagtt cctcatgggg cgcctgctcg gtaatattct
     1501 caacaatatg gcctactatc aggagtgttc cgacatcctc atgcagttgg ggtataatct
     1561 tgaagatatc cgggagcttg agcccgacat gggtctcggg aacgg
//
LOCUS       DAOQUE010000579         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_105498_length_1592_cov_3.646064, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000579 DAOQUE010000000
VERSION     DAOQUE010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105498_length_1592_cov_3.646064"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..1034
                     /locus_tag="PKH10_13215"
     CDS             12..1034
                     /locus_tag="PKH10_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNT29123.1"
                     /translation="MSNTIVSLSPHAKGPLSTEKVMWGVVIALLPSFAFSAYFFGIRA
                     IVVTLVATIACILTEFVIQKYLMRTTVTAFDGSAAITGILLAFNVPSSIPIWQMVLGA
                     VFAIAIGKMVFGGLGRNPFNPALLGRAFMLASFPVDMTTWPIPVKTQWLWEMGKKVDG
                     TTAATPLGFFKEGIKMGGSMGDLVAQRPELYDYFNLFIGNVGGCIGEVSSFAILLGGI
                     YLLYKKIITWHIPVFYLGGLALITGIFWWMEPAKYADPLFHLFAGGAMLGAWFMATDM
                     VTSPMTVKGQILFAVCGGLLCGLIRLFGAFPEGCSYSILIMNAFVPLIDKYIKPTRFG
                     KEVNYG"
     gene            1027..>1592
                     /locus_tag="PKH10_13220"
     CDS             1027..>1592
                     /locus_tag="PKH10_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNT29124.1"
                     /translation="MAKKLESNFVNMFVVLLVIGLVAALALGFTYSKTRPEIEAAALK
                     KQEKIITTVLDGISYDNKPLAEKYAVDGFNGLELYPAKQGGELVGVAVKTVSPSGYSG
                     NVWLMVGFKPDGTINKTAVLEQKETPGLGTKMDTPWKDQYNGKNPGTNNLKVKNEGGE
                     IDAITAATISSRAFSEAVQKAYDAFKKGG"
BASE COUNT          322 a          507 c          456 g          307 t
ORIGIN      
        1 cggagcaaga aatgagcaac actatcgtat cgctctcccc gcacgccaaa gggccgctct
       61 cgaccgaaaa ggtgatgtgg ggcgtcgtca tcgccctcct gccttcgttc gccttctcgg
      121 cctacttttt cggcatccgg gccatcgtcg tgacgctcgt cgccacgatc gcctgtatcc
      181 tgaccgagtt cgtcatccag aagtacctga tgaggacgac ggtcaccgcg ttcgacggtt
      241 cggccgccat caccggcatc ctgctcgcct tcaatgtgcc gtcgagcatc ccgatctggc
      301 agatggtgct cggcgctgtc ttcgccatcg ccatcggcaa gatggtgttc ggcgggctcg
      361 gccgcaaccc cttcaacccg gcgctcctcg gccgcgcctt catgctggca agtttcccgg
      421 tcgatatgac cacttggccg ataccggtca agacccagtg gctctgggag atgggcaaaa
      481 aggtggacgg caccaccgcc gcgacgccgc tcggattttt caaagagggg atcaagatgg
      541 gcggttcgat gggagacctc gtcgcgcaac gcccggaact gtacgattac ttcaacctct
      601 tcatcggcaa tgtcggcggc tgtatcggcg aggtgagctc cttcgcgata ctgctcggcg
      661 gcatctacct gctctacaag aagatcatca cctggcacat cccggtgttc tacctcggcg
      721 gcctcgctct catcaccggc atcttctggt ggatggaacc ggcgaaatac gccgacccgc
      781 tgttccacct gttcgccggc ggcgcgatgc tgggcgcgtg gttcatggcg accgacatgg
      841 tcaccagtcc catgacggtc aaaggacaga tactcttcgc ggtgtgcggc ggcctgctgt
      901 gcggccttat ccgcctgttc ggcgcgttcc cggaagggtg ctcctactcg atactcatca
      961 tgaacgcctt cgtcccgctc atcgacaagt acatcaagcc gacccgtttc gggaaggagg
     1021 tgaactatgg ctaagaaact cgaatcgaac ttcgtgaaca tgttcgtcgt cctgctggtc
     1081 atcgggctgg tcgcggcgct cgcgctcggc ttcacctact ccaagacccg gcccgaaata
     1141 gaggcggcgg cgctcaaaaa gcaggagaag atcatcacga cggtgcttga cggcatatcc
     1201 tacgacaaca aaccgctcgc cgagaagtac gcggtcgatg gcttcaacgg acttgaactt
     1261 taccccgcca aacagggggg cgaactcgtc ggcgtcgcgg tcaagacggt ctctccttcc
     1321 ggttattcgg gcaatgtctg gctcatggtc gggttcaagc ccgacggcac catcaacaag
     1381 accgcggtgc tcgagcagaa agagaccccc gggctcggga ccaagatgga caccccctgg
     1441 aaagaccagt acaacggcaa gaaccccggg accaacaatc tcaaggtcaa gaacgagggc
     1501 ggcgaaatag atgcgataac ggccgccacc atcagttccc gcgccttttc cgaagcggtc
     1561 cagaaagcct atgacgcctt caagaaggga gg
//
LOCUS       DAOQUE010000580         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_105716_length_1590_cov_4.345928, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000580 DAOQUE010000000
VERSION     DAOQUE010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105716_length_1590_cov_4.345928"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="PKH10_13225"
     CDS             <1..1460
                     /locus_tag="PKH10_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNT29125.1"
                     /translation="QFPRSDAVKDATDWRAGLLRAMSHTRKRDHQDAIEIYRNLEDRM
                     SAFDLLEEDIADLYRWSGYSYAALGKNPEAIAAYLTGYGRLAASDRGDSFLYHAADME
                     RLTENWPRAEELYLRILAEYPLSTHRSTVEFLVFWIRYLQKRYDEATAALAGILQSSL
                     RNSYDAQRARYWQGRIDEKNDRDPEAIRAFCDIAREQPLSYYGMLAASRLRVKNIGCD
                     IPSNARASAFPMRSGHRFLPEMQWVTALWAAGQPATAAGLLYLAQGTVAGAGAPEDRL
                     LAAHLAQELSLFGLAGMFARSLPTDYQFSGDIAKMQYPVGYEREIVPHAALYRVPLLF
                     VVALARQESNFETGAVSSSNAVGLLQLLPGTANLLAKEEGYGGKVTNDLLKKPFTNAR
                     FGVKFLGGLLKRYQGNMALAAAAYNAGPGKADRWIKRMAEMEMDELVENIPIFQTRNY
                     VKKVLSNYAAYHYLYEGAIYDSFEFALPGKPVSQN"
     gene            complement(1457..1579)
                     /locus_tag="PKH10_13230"
     CDS             complement(1457..1579)
                     /locus_tag="PKH10_13230"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNT29126.1"
                     /translation="MTTLSVTPDGLSAIGPVAVTLAQAEGLAAHAEAVTARLQV"
BASE COUNT          295 a          506 c          522 g          267 t
ORIGIN      
        1 agcagttccc gaggagcgat gcggtcaagg acgcgaccga ctggcgcgcc ggtctgctac
       61 gggcgatgtc gcatacccgg aagcgggacc atcaggatgc gatagagata taccggaacc
      121 tcgaggatcg catgagcgcc ttcgacctgc tcgaggagga catcgccgac ctgtatcgct
      181 ggagcggcta ctcgtacgcc gcgctcggga agaaccccga ggcgatcgcc gcctacctga
      241 ccggttacgg gcggcttgcc gcgtccgaca gaggcgattc gttcctctac cacgccgccg
      301 acatggaacg gctcaccgag aactggccgc gcgcggaaga gttgtacctg cgcatcctcg
      361 ccgaatatcc cctgtcgacc caccggagca ccgtcgagtt cctcgtgttc tggatacgct
      421 atctgcagaa gcgctacgac gaggcgaccg ccgcgctcgc gggcatcttg cagagttcgc
      481 tgcgcaacag ttacgatgcc cagcgagccc gatattggca ggggcgcatc gatgaaaaga
      541 acgaccggga ccccgaggcg atacgggcgt tctgcgacat cgcgcgcgaa cagccgcttt
      601 cctactacgg catgctagcg gcgtcgcggc tccgcgtgaa gaacatcgga tgcgacatcc
      661 cttcgaatgc tcgggcttcg gccttcccga tgcggagcgg ccataggttc ctgcccgaga
      721 tgcagtgggt gaccgcgctg tgggccgccg gtcagccggc gacggcggca gggcttttgt
      781 atcttgcgca ggggacggtg gcgggcgccg gagcgccgga ggaccggctg ctcgccgccc
      841 atctggcaca ggaactgtcg ctgttcgggc ttgcgggcat gttcgcgcgg tcgctgccga
      901 ccgactacca gttctccggc gatatagcga agatgcagta cccggtcgga tacgagcggg
      961 agatcgtgcc gcacgcggcg ctgtaccgcg tgccgctcct gttcgtcgtg gcgctcgcgc
     1021 gacaggagag taacttcgag acgggggcgg tctcctcgtc caatgcggtc gggctcctgc
     1081 aacttcttcc cggcacggcg aacctgctcg caaaggagga ggggtacggc ggtaaggtga
     1141 cgaacgacct gctgaaaaag ccgttcacca acgcgcggtt cggcgtcaaa ttcctcggtg
     1201 gcctgctgaa gcgctatcag ggcaacatgg cgctcgcggc agccgcctac aacgcggggc
     1261 cgggcaaggc cgatcgctgg atcaagcgta tggccgagat ggaaatggac gaactggtcg
     1321 agaacatccc gatattccag acccgcaact atgtgaaaaa ggtcctctcg aactacgccg
     1381 cctaccatta tctttacgag ggggcgatct atgattcctt cgagttcgcg ctaccgggga
     1441 agccggtgtc acaaaattag acctgcaatc gcgccgtgac cgcttcggcg tgagccgcga
     1501 gcccctcggc ctgcgcgagc gtcaccgcca ccggcccgat cgccgagagc ccgtcggggg
     1561 tcaccgacag cgttgtcacc accttgagga
//
LOCUS       DAOQUE010000581         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_106081_length_1587_cov_3.192559, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000581 DAOQUE010000000
VERSION     DAOQUE010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106081_length_1587_cov_3.192559"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKH10_13235"
     CDS             <1..226
                     /locus_tag="PKH10_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNT29127.1"
                     /translation="CYTTHGRKQKKKYKSNLDMMKWQKDSAVPIAKARDMKPEELAGK
                     FVTGILHEVERPEYSDRYAGLITSLKGGEK"
     gene            223..768
                     /locus_tag="PKH10_13240"
     CDS             223..768
                     /locus_tag="PKH10_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT29128.1"
                     /translation="MKYEIRFSGSGGQGVITASVILGTAAALHDGKNVIQSQSYGPEA
                     RGGATKADVIISDAKIGYPKARHIDHLVSLTQKAAVKFIKTLKKGGTVIIDSDFVDLP
                     DSPDYKIFRLPMTSSSVAKFNRTTTLNVIALGAFAEVAGLVSADAVKKAVLENSPAGF
                     EEMNIASLEMGRELARAAKKG"
     gene            783..>1587
                     /locus_tag="PKH10_13245"
     CDS             783..>1587
                     /locus_tag="PKH10_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29129.1"
                     /translation="MPLLILCLFLFIGCSEQPGPSPDADGSADTLIDDVSMPDEMVCD
                     LGLTPPAAPALPAPVDWGVCPDGWDDTTDGEHRYCLPRVAVDCPAGTMPVIGRIDCVA
                     ICDDREPPAGATVVDVAADADLQNLIDTAPDGSIIRLAAGAYPPVTLSDRELTIVGAC
                     PGEATLGGLSVDGGTVAVRGVAVRDATGWGVSVEGAGTLAAERLYITGGGEETMGALF
                     LTDTAEVSLNGCSIDGALHVGIRGAGESAKLEATRLLVRGIVSDATVDRG"
BASE COUNT          306 a          478 c          522 g          281 t
ORIGIN      
        1 ctgctatacc acccacggac gcaagcagaa gaagaaatac aagagcaacc tcgacatgat
       61 gaaatggcag aaggacagcg cggtgcctat cgctaaggca cgcgatatga agccggaaga
      121 actcgccggg aagttcgtca ccggcatact tcacgaggtg gaacggcccg aatactccga
      181 ccggtatgcc ggactcatca catcgctcaa gggaggcgaa aaatgaaata cgagatccgt
      241 ttctccggca gcggcgggca gggggtcatc acggcgtcgg tgatactcgg cacggcggcg
      301 gcgcttcacg atggcaagaa tgtcatccag tcgcaatcct atggtcccga ggcgcgcggc
      361 ggcgcgacca aggccgatgt catcatcagc gacgccaaga tagggtatcc caaggcgcgc
      421 catatcgatc atctcgtgag tctcacgcag aaggcggcgg tgaagttcat aaagacgctt
      481 aaaaagggcg gcacggtcat catcgacagc gatttcgtcg atctgcccga ttcgcccgac
      541 tacaagatat tccgcctgcc gatgacatcg agttccgtcg ccaaattcaa ccgcaccacg
      601 acgctcaatg tgatagcgct cggcgccttc gccgaggtcg cgggtctggt gagcgccgat
      661 gcggtgaaga aggcggtgct cgagaattcg cccgcggggt tcgaagagat gaatatcgct
      721 tcgctcgaga tgggccgcga actggcgcgc gccgcgaaga aaggctgacg acaccgcttc
      781 ccgtgccgtt acttatcctc tgcctcttcc tttttatcgg ttgttctgaa caacccggtc
      841 cctctcccga tgccgacggg agcgccgaca cccttatcga tgatgtgtcg atgcccgacg
      901 agatggtctg cgatctcggg ctgacgccac ccgctgcgcc ggcgcttccc gcgccggtcg
      961 actggggcgt ctgtcccgac ggatgggacg atacgaccga tggcgaacat cgctactgcc
     1021 tgccgcgtgt cgccgtcgat tgtcctgccg ggacgatgcc ggtcatcggg cggatcgatt
     1081 gcgtggcgat atgcgatgac cgcgagccgc ccgcgggggc gaccgtcgtc gatgtcgcgg
     1141 cggacgccga tctgcagaac ctcattgata ccgcgcccga cggttcgatc atacgtcttg
     1201 cggcgggcgc atatcccccg gtaacgcttt cggatcgaga actcaccatc gtcggcgcct
     1261 gtcccggaga ggcaacgctc ggcgggcttt ccgtcgacgg cggcacggtc gcggtgcggg
     1321 gtgtcgcggt gcgcgacgcg acggggtggg gggtgtcggt ggagggggcg gggacgctcg
     1381 cggcggaacg cctttacatc accggcggcg gtgaagagac aatgggagcc ctctttctga
     1441 ccgatacggc agaggtctcg ctcaacggtt gctcgatcga cggggcgttg catgtcggta
     1501 tccgcggtgc gggagagagc gcgaaactcg aggcgacgcg tctcttggtg cgcggcatcg
     1561 tttcagacgc cacggtcgac cgcggca
//
LOCUS       DAOQUE010000582         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_106199_length_1586_cov_3.467668, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000582 DAOQUE010000000
VERSION     DAOQUE010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106199_length_1586_cov_3.467668"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PKH10_13250"
     CDS             complement(<1..385)
                     /locus_tag="PKH10_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29130.1"
                     /translation="MKACYFCGKLTPEVVGTPEKEKKEVPAPKRSADEAIFATTTAAM
                     AQKKGAGKTAFRIVLVAVGLILLFLVGKEAMVLFDGSSDEPRRKVFYDPVNGLLWQRS
                     PTMPMNYENAARYCEKMQVGAMAGWR"
     gene            complement(445..1530)
                     /locus_tag="PKH10_13255"
     CDS             complement(445..1530)
                     /locus_tag="PKH10_13255"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT29131.1"
                     /translation="MKSLLFAIVLLGAALLSAQEKKQRLAVLEIEDRTGALPKELMTT
                     ASDYLRARLVAVNSFLLVPKDNVMRAVIKEQKKESWRDCYDPACRVDLGHALAADTVL
                     ASMVMRLADDHVVTVELVDIAKEVTIKGVSVTFDGSEEGLKKALDRVADRIAGKRPEK
                     VSPAPADKVDPTEKLKGAVDLQEKPKVEKPLAPPEKKKPDANTYDVRGEVIIDRDTGH
                     SWQRSHGGPIDLEKARSYCEDLVLGGFTDWRIPTISELRTLIIGCPNTVPDGICPVDD
                     ACAGTGECRERLKEKDCACKAHKGPGEKGSYWKKGVWRYNGGRYDLFWSRSLISGPYE
                     EAWGVLFSEGIVTTVDVSGRYYVRCVR"
BASE COUNT          285 a          521 c          433 g          347 t
ORIGIN      
        1 gacgccagcc agccatcgcg ccgacctgca tcttctcgca atatcgcgcg gcgttctcgt
       61 agttcattgg catcgtcggc gatctctgcc acaggagccc gttgaccgga tcgtagaaaa
      121 ccttccgccg gggctcatcg ctactgccgt caaaaaggac catcgcctct tttcccacga
      181 ggaagagtag gatcagcccc acggcgacca gtacgatacg aaaggcggtc ttccccgctc
      241 cttttttctg ggccatcgct gcggtagtgg tggcgaagat cgcttcgtcg gccgaccgtt
      301 tgggcgcggg gacctccttt ttctccttct caggcgttcc gacgacctcg ggcgtaagtt
      361 ttccacagaa ataacacgcc ttcaccgcgt tcggtaaggg cctcttacat ttcggacata
      421 tcgccatggg acctccggtt ccgttcatcg tacgcaacgg acatagtagc ggcctgaaac
      481 atcgacggtg gtaacgatgc cctcgctgaa aaggacgccc cacgcctcct cgtacggtcc
      541 cgatatgagc gaccggctcc agaaaagatc gtatctcccg ccgttgtacc tccagacacc
      601 ttttttccag taacttccct tttcccccgg acccttgtgc gccttgcacg cacagtcctt
      661 ctctttcagt ctctcgcggc actccccggt cccggcacag gcgtcgtcga ccgggcagat
      721 gccatcgggc acggtatttg gacacccgat gatgagcgtc ctgagttccg atatggtggg
      781 gatacgccag tcggtgaaac cgccgagcac gaggtcctcg cagtaggatc gcgccttctc
      841 gagatcgatc gggccgccgt gggagcgttg ccacgaatga ccggtgtcgc ggtctatgat
      901 gacctcgcca cgcacatcgt aggtgttcgc gtccggtttt ttcttttcgg ggggagcaag
      961 cggcttttcg accttcggct tttcctgcag atcgaccgcc cccttgagtt tttcggtcgg
     1021 atcgaccttg tcggccgggg caggagacac cttttccggc ctcttccctg cgatgcggtc
     1081 ggcgacacgg tcgagtgcct tcttcaatcc ctcctcgcta ccgtcgaagg tcaccgatac
     1141 ccctttgatg gtcacttcct tggcgatatc gaccagttcg acggtgacaa catggtcgtc
     1201 cgcgagccgc ataaccatcg aggcgagcac ggtatcggcg gcgagtgcat gaccgaggtc
     1261 cacccggcaa gcggggtcat aacagtcgcg ccaactctcc ttcttctgct ccttgatgac
     1321 ggcacgcatc acattatcct tcggcacgag gaggaaactg ttcacggcga ccagccgcgc
     1381 ccgcagatag tcggaggcgg tggtcatgag ttccttgggc agtgcgccgg tccggtcctc
     1441 tatctcgagc accgcgagcc gctgtttttt ctcctgagcc gataacaagg ccgcgcccaa
     1501 caggacgatc gcgaacaaca ggcttttcac catgcccctc ccttgataaa cccgctcaga
     1561 acatgagcga gcagccgcag cccgcc
//
LOCUS       DAOQUE010000583         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_106503_length_1583_cov_4.193717, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000583 DAOQUE010000000
VERSION     DAOQUE010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106503_length_1583_cov_4.193717"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..1184)
                     /locus_tag="PKH10_13260"
     CDS             complement(300..1184)
                     /locus_tag="PKH10_13260"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNT29132.1"
                     /translation="MTVRAPKEVALVVRERLLKRSRQTGEDFQFLLQHYAAERFLYRL
                     GCSPHRERFVLKGAMLFALWGGSFYRPTRDLDFTGYGEIATGAILDAMRDICTLVVED
                     DGVVFREDTLKAGPIRDEAEYGGIRVVFQAGIGTARIRMQVDIGFGDAIEPRAALADY
                     PTLLDLPVPHIRVYPYEAVVAEKLHAMVTLGEINSRHKDFYDLYVMVSRLPFDGGRLS
                     RAVAATFERRRTPITKDVPVALTARFYADAGRGEQWRAYLSRNGLAGVPTDLVAVGEK
                     IMDFLRPAWAALGGNTER"
     gene            complement(1181..1576)
                     /locus_tag="PKH10_13265"
     CDS             complement(1181..1576)
                     /locus_tag="PKH10_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29133.1"
                     /translation="MPGGIVCLLSALQMQEIGTQLPHEIWIALDRKARRPRRLPAKVR
                     IVRFSGPMLTHGVAKRNILGVPVHFTDPARTVVDCFRYRSKIGLDVALEALREVIRSR
                     KATVDGIMRAAEACRARTLVRHYLEALLS"
BASE COUNT          336 a          575 c          395 g          277 t
ORIGIN      
        1 ttcttccccc tttcttgacg ctcatcaaga aaggggtcgg tgtagcacgc gaagcggcgc
       61 cgaaactgct attaattaaa cccttccccg ggcgaacaca tcggttcgcc cctacggatc
      121 ccatcacacc cccaccgcgt cgcggagccc cctgcgacaa ggggccaatc ccgtgtatcg
      181 ggcgtcccgt tgggagcgcc cctacaattc ggtattcccc ttcaccgttc accgttcact
      241 cctcaccgtc ttatgcctca cgcctcacgc ctcacgcctc acgattcacg attcacctct
      301 cacctctccg tattccctcc aagcgccgcc cacgccggtc tcagaaaatc catgatcttt
      361 tctcccaccg ccacaagatc ggtcggaaca cccgccagac cattccggct cagatacgca
      421 cgccactgtt cgccacggcc ggcatcggca taaaaacggg cggtaagggc aacaggaaca
      481 tccttcgtta tcggcgtacg ccgccgttca aaggtcgcgg caacggcgcg ggacagtcgc
      541 ccaccgtcga acggaaggcg agacaccatc acatagagat cataaaagtc tttgtggcgg
      601 ctgtttatct ctcccagcgt caccatcgcg tggagttttt ccgccacgac cgcctcgtac
      661 ggatagacgc gaatatgcgg caccggaaga tcaagaagcg tcggatagtc ggcaagagcg
      721 gcgcgcggct ctatcgcatc gccgaacccg atatccacct gcatgcggat gcgtgccgtc
      781 ccgatcccgg cctgaaagac cacccgtatc ccgccgtact ccgcctcgtc acggatcggc
      841 ccggccttca gtgtatcttc ccggaacacc accccgtcat cctcgacgac cagcgtacag
      901 atatcgcgca tcgcgtcgag tatcgccccc gtcgcgatct caccgtaccc ggtaaagtcc
      961 agatcgcgcg tcggccggta gaacgaccct ccccacaacg cgaacaacat cgcccccttc
     1021 agcacgaaac gctcccgatg gggcgaacaa cccagacgat acaggaaccg ttccgccgca
     1081 taatgttgga gaagaaattg aaagtcttcc ccggtctgcc tgctgcgctt cagcaaccgt
     1141 tcgcgcacca ccagcgcgac ctctttcggc gcccgaacgg tcatgacaac agcgcctcaa
     1201 gatagtgtct caccagcgtc cgggcgcgac acgcctccgc cgcccgcatg ataccgtcaa
     1261 ccgtcgcctt gcgcgaacga atgacctccc gcagagcctc aagcgcgaca tcaagcccta
     1321 tcttcgaccg atagcggaaa cagtccacca ccgtacgggc tggatcggtg aaatgcaccg
     1381 ggacaccgag gatattcctc tttgccacgc catgcgtgag catcggcccg gaaaaacgga
     1441 cgatacgcac tttggccggc agacggcgcg gcctgcgcgc cttgcgatcc agagcgatcc
     1501 atatctcatg cggcagttgc gtgccgatct cctgcatctg aagcgccgaa agcagacaga
     1561 cgatccctcc cggcaccgcg gcg
//
LOCUS       DAOQUE010000584         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_106846_length_1580_cov_5.059672, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000584 DAOQUE010000000
VERSION     DAOQUE010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106846_length_1580_cov_5.059672"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PKH10_13270"
     CDS             complement(<1..821)
                     /locus_tag="PKH10_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29134.1"
                     /translation="MKKEFFRAALLIGVTVIVLAASSCAPTDEDPGTGSYCEEDADCG
                     DTAIYFCDTANNECKVKAGVDIDQEGTADTDEGGVTDQEDAEYDPTADDGTETPDIDQ
                     SGCDYTVCNGNGKCQPDNSCKCYQGYAGTYCDECSPGYIGYPDCYFKMCEPNTRTCKD
                     DLTIQQCDEKGMDWAEVESCSGEATCFEGECLTECGRAEAEESYVGCEYWGAFLQNLM
                     EYEGGNYAVVVANPNDTDVTVTVYISGDTQIAQFTVAPGQINSYTFDQSYLIPGPG"
     gene            complement(850..>1580)
                     /locus_tag="PKH10_13275"
     CDS             complement(850..>1580)
                     /locus_tag="PKH10_13275"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HNT29135.1"
                     /translation="SCGSSCYGPDLSGSMIAADRKIAVFGGHVCTFIPASTYACDHLE
                     HQIFPLRSWGKEFAAVRTKPRNAEADYFRILASKDGTTVSLTGGESGSVALSAGQVHE
                     FSTTADFVVTANEPIMVAQFLASQDAGADTGDPAMMLIAPNEQMRQDYIFLVAPNYDY
                     DRLTIVSPADNQITLDGGAVMDSNTFTQIPGTPWYRQYVDTDDGAHTLTATKPVALYV
                     YGFSQYVSYAYTAGLDLQKINDQE"
BASE COUNT          269 a          456 c          502 g          353 t
ORIGIN      
        1 cccggtcccg ggatgagata ggactggtcg aaggtgtagg aatttatctg tcccggtgcg
       61 acggtgaact gggcgatctg cgtgtcgccc gagatgtaga ccgtcacggt cacatcggtg
      121 tcgttcgggt tcgcgacgac gaccgcgtag ttgccgccct cgtattccat gaggttctgc
      181 aggaaggcgc cccagtattc gcacccgaca taactttcct ccgcttcggc gcgtccgcat
      241 tcggtgaggc attctccctc gaagcaggtc gcctcgccgc tacaggattc gacctcggcc
      301 caatccatcc ccttttcgtc gcattgttgt atcgtcaggt cgtccttgca ggtccgggtg
      361 ttcggttcgc acatcttgaa atagcagtcg gggtagccga tgtagcccgg ggagcattcg
      421 tcgcagtagg tccccgcgta accctgataa catttgcacg aattgtccgg ctggcatttt
      481 ccgttgccgt tgcagacggt atagtcgcac cccgactgat cgatgtcggg cgtctcggtg
      541 ccgtcgtcgg ccgtggggtc gtactccgcg tcttcctgat cggtcacgcc gccctcgtcg
      601 gtgtcggccg tgccttcctg atcgatgtcg acgccagcct tgaccttgca ctcgttattc
      661 gccgtgtcgc agaagtagat ggcagtgtcg ccgcagtcgg cgtcctcttc acagtaactg
      721 ccggtgccgg ggtcttcgtc ggtcggcgca caggaggacg cggccagaac gatcacggtc
      781 acgcctatca gcaaggcggc gcgaaaaaac tcttttttca tgttacatcc tccgggaaaa
      841 gtccttgtct tattcctgat cgtttatctt ctgcagatcg agcccggcgg tgtaggcgta
      901 ggaaacatac tgcgagaagc cgtatacata gagcgcgacc ggtttggtgg cggtcagcgt
      961 gtgggcgcca tcgtcggtgt ccacatactg gcgataccag ggcgttcccg gtatctgcgt
     1021 gaaggtgttg gaatccatga cggcaccgcc gtcgagcgtt atctggttgt ccgccgggga
     1081 aacgatggtg agtcggtcgt aatcgtagtt cggcgcgacg aggaagatgt agtcctgccg
     1141 catctgctcg ttcggggcta tcagcatcat cgccgggtcg ccggtgtcgg ctcctgcgtc
     1201 ctgcgaggcg aggaattggg ccaccatgat aggctcattc gcggtcacga cgaaatcggc
     1261 ggtggtcgag aactcgtgca cctgtccggc gctcagcgcc acggagcccg attcgccacc
     1321 ggtcaatgaa acggtcgtgc cgtccttcga ggcgaggatg cgaaaatagt cggcctcggc
     1381 gttgcgcggt ttggtgcgga ccgcggcgaa ttccttgccc catgagcgga gcgggaatat
     1441 ctggtgttcg agatggtcgc aggcgtaggt cgaggcgggg atgaaggtgc agacatgacc
     1501 gccgaacacc gctatcttcc tgtcggcggc gatcatgctc cctgaaagat cggggccgta
     1561 acaggaactg ccgcacgatg
//
LOCUS       DAOQUE010000585         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_107331_length_1576_cov_4.851414, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000585 DAOQUE010000000
VERSION     DAOQUE010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107331_length_1576_cov_4.851414"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..809)
                     /locus_tag="PKH10_13280"
     CDS             complement(162..809)
                     /locus_tag="PKH10_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT29136.1"
                     /translation="MTTKVAFIGGGETTLAFLRFFERTPGIEVVMVTDEFRDAPALRY
                     AAEHQVRISTNIMDAIRYPEIDLIIELTDNRADVLSVINDNLPPRTTLLTHRHARVMY
                     DLLAAIIENEFATIEERFMLNIKDIKKSITDFAAITKNIDILAINASIEAARAGETGK
                     GFAVVANSIKDLVKNSRDTLQHVRSVLEKLTLIHKNMQETRSTLKTKERSDPLTY"
     gene            complement(835..1455)
                     /locus_tag="PKH10_13285"
     CDS             complement(835..1455)
                     /locus_tag="PKH10_13285"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNT29137.1"
                     /translation="MRSSFLFLLFLLLPCLSFAVDAPAPATEGLAEGSPLVDFGMRVV
                     DLESGKLKNLLWLTDFVGRPPQEKNGSATAERKKALVLNFYANWCKPCIEEMPLLQKL
                     HDRYSGKGVQFLGVNFRNDDENPDKVLAGTIDILKKEKITYPILFDRFTTRNQLIYMG
                     SKAILPTVVIFNADGVMVKKLQGEKTKNIKEIETTIQKLTGEAARY"
BASE COUNT          330 a          384 c          465 g          397 t
ORIGIN      
        1 ggcgaggtcg attgggcccg cgcgatggat gatgtcaaga tgcgctccat cgagttttat
       61 gactcggtcg aacgggccta tgataccgat acggcgcgcc gggtcctcga tatcgcgttc
      121 cctactcctg agaccaaaag gttcttcttc tcgggaagcg gtcaataggt aagaggatcg
      181 ctccgttcct tggtcttgag ggtgctgcgg gtctcttgca tgttcttatg gatgagagtg
      241 agtttctcca gcaccgatct gacgtgctga agcgtgtcgc ggctgttctt gaccagatcc
      301 ttgatggagt tcgccaccac ggcgaatccc ttgccggtct ctcccgcccg cgccgcttca
      361 atggacgcgt tgatcgcaag gatatcgatg ttcttggtga tggcggcaaa gtcggttatc
      421 gatttcttga tgtccttgat gttgagcatg aacctttctt ctatggtcgc gaactcgttc
      481 tcgatgatcg cggcaagcag atcgtacatg acacgggcat ggcggtgagt tagcagtgtg
      541 gtgcgcgggg gaaggttgtc gttgatgacc gaaagcacgt cggcgcggtt atcggtgagt
      601 tcgatgatga ggtcgatctc ggggtaacgg atggcatcca tgatgttcgt cgatatacgc
      661 acctggtgct cggcggcata acggagtgcg ggggcgtccc ggaactcgtc agtgaccatc
      721 accacttcga tgcccggcgt cctttcaaag aaacggagaa aggcgagggt cgtttcgcca
      781 ccgccgatga acgcgacctt tgtcgtcatg cttgatcctc ctttccttta agggctaata
      841 acgtgccgct tctccggtaa gtttttgaat ggtcgtctct atctctttga tgttctttgt
      901 cttttccccc tgcaattttt tgaccatcac gccgtcggca ttgaatataa cgaccgtggg
      961 caggatggct ttggagccca tgtagataag ttgattgcgt gtcgtaaagc ggtcgaacag
     1021 tatggggtag gttatcttct cttttttcaa gatatcgatc gtgcctgcaa gtaccttatc
     1081 ggggttctcg tcgtcgttgc ggaaattaac gccgaggaac tgcacgccct taccggagta
     1141 ccggtcgtgc agtttctgga gtaacggcat ctcctcgata cagggtttgc accagttggc
     1201 gtagaaatta aggacaaggg cctttttcct ttccgcagtg gccgatccgt ttttttcctg
     1261 aggcggcctc ccgacaaaat cggtgagcca gagcaggttc ttgagttttc cgctttccag
     1321 gtcgacgacc cgcataccga aatcgaccag aggagatcct tcggcgaggc cctcggtcgc
     1381 gggagcggga gcgtcgaccg caaaggaaag acagggcaac aacaagaaaa gaagaaaaag
     1441 aaaggaagaa cgcattgcgc ctccggagcg gttggaaaaa tacgggagga tgataccatg
     1501 ccggagagaa aatgccactt tttggcgaag agattcttcg tgatcgacgg gggatatctc
     1561 gtcaaaagag gacgat
//
LOCUS       DAOQUE010000586         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_107338_length_1576_cov_4.241289, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000586 DAOQUE010000000
VERSION     DAOQUE010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107338_length_1576_cov_4.241289"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1318
                     /locus_tag="PKH10_13290"
     CDS             167..1318
                     /locus_tag="PKH10_13290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT29138.1"
                     /translation="MTDIRRTPFYARQYIARRRKSQSNPDTLLSCFSPADRAPCAESE
                     TGGSPLKERCDILILGAGPAGLSCAAELAGSGASVVVLERQRAVGPKACAGALSRLMP
                     ADLTPPSALRFDEQVVEIAGKEHRLRLETPRFTIPRETLASYQLAKIPNGAGVEIMLG
                     VRARMRDRHTIATDGGDEWSADSIVGADGALSIVRRSLGLPTRRSLGIYREVRTISKD
                     YALLLDAARYGTGYLWSFPHRDRVNIGIYFDPGRLAPAVARGLLDGEIARRFPGMPVS
                     PPKSGWVAHRYAGVEHEGIYLIGDAAGLALRATGEGISTAVVSGREIGRKLKDRRYAM
                     PELARLLAFKKKQERLFSLLERFPGMQDTLYRLFITAKRLPGGQRFFER"
     gene            complement(1315..>1576)
                     /locus_tag="PKH10_13295"
     CDS             complement(1315..>1576)
                     /locus_tag="PKH10_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006008043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD-dependent epimerase/dehydratase"
                     /protein_id="HNT29139.1"
                     /translation="ATPESFTGPVNLGNPHEVTMRELAASIIRLTNSRSRLTLLPLPQ
                     DDPKRRCPDIGLAKRELGWEPRTPFEEGLAATIAHFKKEGRD"
BASE COUNT          289 a          504 c          523 g          260 t
ORIGIN      
        1 cgcaccgaga ccggtgaaca ggatggagaa ggcggccgtc agcgatatcg cgaagatcat
       61 ctggaacggg aagacatacg agagcatccg cacggtgagc acctgcaacg acgagcagag
      121 cgtcgcgtag acgatagccc ggaacagggc gaggcgggcc ggggtcatga cggacataag
      181 gagaactccc ttttacgcgc gtcagtatat cgcccggcgc cgcaagagtc aatcaaaccc
      241 ggataccctc ctttcttgct tttcaccggc ggacagggca ccatgcgcgg agagcgaaac
      301 gggaggatcc cctctgaaag aacggtgtga catactcatc ctcggcgccg gcccagccgg
      361 gctttcctgc gcggcggaac tcgccggttc gggcgcgtcg gtggtagtgc tggaaaggca
      421 gcgggccgtc ggcccgaaag cctgcgccgg cgccctctcg cggctgatgc cagccgatct
      481 tacgcctccc tcggccctcc ggttcgacga acaggtcgtg gagatagcgg gaaaagagca
      541 ccgcctccgc ctcgaaacgc cacggttcac catcccccgc gagacactcg cgagttatca
      601 actggcgaag atacccaacg gggcgggggt ggaaatcatg ctcggcgtcc gggcccggat
      661 gagggaccgc cacaccatcg cgaccgatgg cggggacgaa tggtcggccg acagcatcgt
      721 gggcgccgac ggcgctcttt cgatcgtccg ccggtcgctc ggcctaccga cgcgccgatc
      781 tctgggcata tatcgcgagg tacgcaccat ttcgaaggat tacgcgctcc tgctcgacgc
      841 ggcgcgctac ggcaccggct acctctggtc ctttcctcac cgcgaccggg tgaatatcgg
      901 catctatttc gatccggggc ggcttgcccc cgccgtcgcc cggggtctgc tcgacggaga
      961 gatagcgcgc cggttccccg gcatgcccgt atcgccgccg aaaagcggct gggtcgcgca
     1021 ccggtacgcc ggcgtcgaac acgaggggat ctatctcatc ggcgacgcgg cgggactggc
     1081 gttacgggcg accggcgaag ggatatccac tgcggtcgtc agcggtcgcg agataggccg
     1141 taaactgaaa gacaggcgct acgcgatgcc cgaactggcg cgactgttgg ccttcaagaa
     1201 aaaacaggaa cggctgttct cgctgcttga acgctttccc ggcatgcagg atacgctcta
     1261 tcggctgttc atcacggcaa aacgactgcc cggcgggcaa cgattcttcg agcgctagtc
     1321 gcgcccctct tttttgaaat gcgctatcgt cgcggcgagc ccctcctcga acggggtgcg
     1381 cggttcccag ccgagttccc gcttcgcgag cccgatgtcg ggacaccgcc gtttcgggtc
     1441 gtcctgcggg agcgggagca aggtcaggcg cgaccgcgag ttggtgagcc ggatgatgga
     1501 cgcggcgagt tcgcgcatcg tcacctcgtg cgggttgccg aggttgaccg ggccggtgaa
     1561 actttccggg gtcgcc
//
LOCUS       DAOQUE010000587         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_107360_length_1576_cov_3.039448, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000587 DAOQUE010000000
VERSION     DAOQUE010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107360_length_1576_cov_3.039448"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..487
                     /gene="nuoE"
                     /locus_tag="PKH10_13300"
     CDS             20..487
                     /gene="nuoE"
                     /locus_tag="PKH10_13300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNT29140.1"
                     /translation="MTEQVLTRYPGAGRDKLIPILQEVQEADGYISREAVALIGRHLG
                     VPASKIYGVATFYNQFRFTPKGKYHIMVCRGTACHVKGSADILERIERTLKIKAGETS
                     RDGLFSIEVVACIGACGLAPVICVNGEYHGKLTGEAVEKIVQSYREREAAHGN"
     gene            477..>1576
                     /locus_tag="PKH10_13305"
     CDS             477..>1576
                     /locus_tag="PKH10_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HNT29141.1"
                     /translation="MATDTLFTLGAEALKRLRRETVDKPVIFVGAGTCGLGAGAAQTL
                     AAVREWLAVSAVDADIVETGCIGLCAEEPLMDVQLPGRARISFKKVTAEKVTRILDDI
                     FAGAVDTKLALVQFDAGDNKGWDGVPTFAEHPFFRRQTRWVLGNCGVIDPESLEEYCA
                     RGGYEALRNTLSWLTPDEAVETVVKSGLRGRGGGGFPTGKKWRFARAEKGELKYLICN
                     ADEGDPGAFMDRAVIEGDPYRLLEGMTIAAYAIGAREAYIYIRAEYPLAVKRLERAIA
                     RAEKHNLLGERILGSDLSLRMHIKMGAGAFVCGEETALINSIEGKRGMPRPRPPFPAV
                     RGLFGKPTVINNVETFANVPEIFRNGAEHFAAV"
BASE COUNT          310 a          491 c          537 g          238 t
ORIGIN      
        1 ggagtcactc atgaacacga tgaccgaaca ggtgctgacg aggtatccgg gggcggggcg
       61 ggataaactc atcccgatcc tgcaggaggt gcaggaggcc gacggttata tctcgcggga
      121 ggcggtcgcg ctgatcggcc gtcatctggg ggtgcccgcc tcgaagatct acggcgtggc
      181 gaccttctat aaccagttcc gcttcacgcc gaaggggaaa taccacatca tggtctgccg
      241 cggcaccgcc tgtcatgtca aagggtcggc cgacatactc gaacggatcg agcggacgct
      301 caagataaag gcgggcgaaa cgagccgcga cggcctgttc tcgatagagg tggtcgcctg
      361 catcggcgcc tgcggactcg cgccggtcat ctgcgtcaac ggtgaatacc acggcaaact
      421 gacgggagag gcggtcgaga agatcgtgca gagttatcgg gaacgggagg cggcacatgg
      481 caactgacac tcttttcacc ctcggcgccg aggcgctgaa aagactccgc cgcgaaacgg
      541 tcgacaagcc ggtcatcttc gtcggcgcgg ggacctgcgg actcggtgcg ggggcggcgc
      601 agacgctcgc cgcggtccgg gaatggctcg ccgtgagcgc ggtcgacgcc gacatcgtcg
      661 agaccggctg tatcgggctg tgcgccgaag agccgctgat ggatgtccag ctccccggcc
      721 gggcgcggat cagtttcaaa aaggtgaccg ccgaaaaggt gacgcggata ctcgatgata
      781 tcttcgcggg ggcggtcgat accaaactgg cgctcgtcca gttcgacgcg ggcgacaaca
      841 aaggctggga cggggtcccg accttcgccg aacatccctt cttccggcgg cagacccgct
      901 gggtgctggg caactgcggc gtgatcgatc ccgaaagcct tgaagaatac tgcgcccgcg
      961 gcggctacga agcgctccgc aacacgctgt cgtggctgac gcccgacgaa gcggtcgaaa
     1021 cggtggtcaa aagcggcctg cgcggacgcg gcggcggcgg cttcccgacc gggaagaaat
     1081 ggcggttcgc ccgcgccgaa aagggcgaac tcaaatatct catctgcaac gccgacgaag
     1141 gggacccggg ggccttcatg gaccgcgccg tcatcgaagg cgatccttac cggctcctcg
     1201 aggggatgac catcgccgcc tacgccatcg gggcgcgcga ggcctacatc tacatccgcg
     1261 ccgaatatcc gctggcggtc aaacggctgg agcgggccat cgcccgcgcc gagaagcaca
     1321 acctgctcgg cgaaaggata ctggggagcg acctgtcgct ccgcatgcac ataaagatgg
     1381 gggccggcgc cttcgtctgc ggcgaggaga cggcgctcat caacagcatc gaagggaaac
     1441 gcgggatgcc gcgtccccgt ccgcccttcc cggcggtgag ggggctcttc ggcaagccga
     1501 ccgtcatcaa caacgtcgag accttcgcca atgtcccgga gatcttccgc aacggcgcgg
     1561 agcacttcgc ggcggt
//
LOCUS       DAOQUE010000588         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_109422_length_1558_cov_3.332668, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000588 DAOQUE010000000
VERSION     DAOQUE010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109422_length_1558_cov_3.332668"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..>1558)
                     /locus_tag="PKH10_13310"
     CDS             complement(136..>1558)
                     /locus_tag="PKH10_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNT29142.1"
                     /translation="MWERFSFYTMRAILVLYLAGVVFADLPGGPQVIRDRALEVYGTY
                     LLLVYLSGLAGGYFADKVLGSRKAIFIGGLVMGAGLFVMMAPQLVFYGLGLVIAGNGF
                     FKPNISTIVSGLYAEHDNRRDGAFTIFYMGINIGAFLAIFSAGYAEGIGNWNLGFVIA
                     GIGMIVSLFVFALGQKHLGTAGFPPNRLVTADTRLVATDARDIALWAVGMIAAVLGVV
                     FLWNHGLAPIWEMIPGMVKLAGGILLIIGAFGGFVATNRDNTREEWERIISIVILSVF
                     VIFFWAGFEQAGGTMNLFAYEQTDRHIFGWEMPATWFQGVNPFFIVLLAPLFSMLWGR
                     LDRSRFPLSTPAKMGVGMIILGVGFVVMYFGEGVAEQYGKAGMGWLVAVYLLHTMGEL
                     CLSPIGLSMVTKLSPVRRVSLMMGVWFACTAIADYLSGRLEAITHAFGINLWATLIAT
                     SIGAGVLLLFLTPMLKKWMHGRA"
BASE COUNT          346 a          489 c          472 g          251 t
ORIGIN      
        1 cggcgcggta atgttcgacg gtgatggtcg ggagtttctt cttctcgagc atctcgttca
       61 tcaccgcgac gccgagccac gcatcgtcaa tgagcgttcc gttccaatcc cagaggatat
      121 gcgaatactt tgacatcagg ccctgccgtg catccatttc ttgagcatcg gcgtgaggaa
      181 gaggagcagc acgcccgcgc cgatggaggt ggcgataagc gttgcccaca ggtttatgcc
      241 gaaggcatgg gtgatcgcct caagacggcc agacaggtag tcggcgatgg cggtgcaggc
      301 gaaccagacg cccatcatga gcgaaacgcg ccgcaccggc gagagtttcg tgaccatcga
      361 aagccctatc ggcgagaggc acagttcgcc catcgtgtgg aggagataga cggcgaccag
      421 ccagcccatc ccggccttgc cgtactgttc cgcgacccct tcgccaaagt acatcacgac
      481 gaagccgacc ccgaggatga tcatacctac ccccatcttg gccggggtcg acagcgggaa
      541 tcgcgaacgg tcgagacgcc cccacaacat cgagaaaagc ggcgccaaca ggacgatgaa
      601 gaacggattc accccttgga accaggtggc gggcatctcc caaccaaaga tgtggcggtc
      661 ggtctgttcg taggcgaaga ggttcatcgt gccccccgcc tgttcgaacc cggcccagaa
      721 gaatatcacg aagaccgaca ggatgacgat gctgatgatc cgttcccact cttcccgggt
      781 attgtcgcga ttggtggcga caaagccgcc gaacgccccg atgataagca gtatgccgcc
      841 ggcgagtttc accatgcccg gtatcatctc ccagatcggc gcgagaccgt gattccacag
      901 gaagaccacc ccgagcaccg ccgctatcat ccccacggcc cagagcgcga tgtcgcgggc
      961 atcggtcgcc accagacggg tgtcggccgt caccaggcgg ttcggcggga aaccggccgt
     1021 gccgaggtgt ttctgcccga gagcgaacac gaaaaggctc acgatcatcc cgatgccggc
     1081 gatgacaaag ccgagattcc aattccctat cccttcggcg tacccggcgc tgaatatcgc
     1141 gagaaaggcg ccgatgttga tgcccatgta gaaaatggtg aaggcgccgt cgcggcggtt
     1201 gtcgtgctcg gcgtacaatc cgctgacgat cgtcgagatg ttgggcttga aaaagccgtt
     1261 gcccgcgatg acgagcccga gcccgtagaa gaccagttgg ggcgccatca tgacgaacag
     1321 gccggcgccc atcaccaacc cgccgatgaa tatcgccttg cgcgaaccga gcaccttgtc
     1381 ggcgaagtag ccgccggcaa gccccgacag atagacgagc aggagatagg tgccgtacac
     1441 ctcgagcgcc cggtcacgga taacctgcgg gccgcccggc aggtcggcga agaccacgcc
     1501 ggcgaggtac agcacgagaa tggcgcgcat cgtgtagaac gagaaccgtt cccacatc
//
LOCUS       DAOQUE010000589         1557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_109526_length_1557_cov_4.342876, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000589 DAOQUE010000000
VERSION     DAOQUE010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109526_length_1557_cov_4.342876"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PKH10_13315"
     CDS             <1..600
                     /locus_tag="PKH10_13315"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HNT29143.1"
                     /translation="GRSHVQMMGQMLKRSASLDEAIAFMQGENHMSVEQIGIADANLG
                     RAGVFEMTAKKTAVREMENGVLWMTNHFVHPDMEALDLDPAGESSLRRFTRFSQLVPP
                     DGTESRYGTFDPPEVVRVLRDRINANSGEESPLGTFDDNRSLATNGAIYAIVFDPGSL
                     HFWAAAGTIPVPEQEFTGFSLGTLLNWPGAIPVDPAILP"
     gene            597..1280
                     /locus_tag="PKH10_13320"
     CDS             597..1280
                     /locus_tag="PKH10_13320"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNT29144.1"
                     /translation="MKKISFIPWPLLLMLLLVPGLVALGKVTGQRTVQSVVKACEKAT
                     DENLMPKFAKAGATYPPKSITLVALKTEEMLELWADGKYIKSYLFTANSGDVGPKLRQ
                     GDYQIPEGIYRIEHLNPMSSFYLSMKVGYPNDFDRARAKEDGRTDLGGDIFIHGSSVT
                     IGCIPIGDGPIEELFTLVAKTGVSKVTVIILPNDVRGGAPFKAPADPPKWLPKLHDEL
                     RKAIAPYKR"
     gene            complement(1299..>1557)
                     /locus_tag="PKH10_13325"
     CDS             complement(1299..>1557)
                     /locus_tag="PKH10_13325"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT29145.1"
                     /translation="AMMTVIFVILMPFIYLSGFVFPVDSMPELFQHLSKVISLTYYLE
                     IVRSLFLKGSGFDVLYKDLVALTILGVVTFAISVVRFRKYI"
BASE COUNT          355 a          470 c          467 g          265 t
ORIGIN      
        1 gggcgcagtc atgtccagat gatgggtcag atgctgaaaa ggtccgcgtc gctcgacgag
       61 gcgatcgcgt tcatgcaggg ggagaaccac atgagcgtcg aacagatcgg tatcgccgac
      121 gcgaacctcg gccgcgccgg ggtcttcgag atgaccgcga aaaagaccgc cgtccgcgag
      181 atggaaaatg gcgtcctctg gatgaccaac catttcgtcc accccgacat ggaggcgctc
      241 gacctcgacc cggccggcga atcgtcgctc cgccgtttca cccgtttctc gcaactggtg
      301 ccgcccgacg gtaccgaatc gcggtacggg accttcgatc cacccgaagt ggtcagggtc
      361 ctgcgcgacc gtatcaatgc caacagtggc gaggaaagcc cgctcggcac cttcgacgac
      421 aaccggtcgc tcgccaccaa cggcgccatc tacgccatcg tcttcgaccc cggcagtctc
      481 cacttctggg ccgcggcggg gaccatcccg gtgccggagc aggagttcac cggcttctct
      541 ctcgggacgc tcctcaactg gcccggcgcg ataccggtcg atccggcgat actgccgtga
      601 aaaagatatc gttcatcccg tggccactgt tattgatgtt gctgcttgtg ccggggcttg
      661 ttgcgctcgg taaagtgacg ggacagcgga cggtgcagtc ggtcgtgaag gcttgcgaga
      721 aggcgaccga cgagaacctg atgccgaagt tcgccaaagc gggcgcgacc tatccgccga
      781 agagcataac actcgttgcg ctcaagaccg aggagatgct ggaactctgg gccgacggca
      841 agtacattaa aagttatctg ttcaccgcca acagcggcga tgtcgggccg aaactgcggc
      901 agggcgacta ccagataccg gaagggatat accgcatcga gcatctcaac ccgatgagca
      961 gtttctacct gtcgatgaag gtgggctacc cgaacgattt cgaccgggcg cgggcaaagg
     1021 aggacgggcg caccgacctc ggcggcgata tcttcatcca cggatcgagc gtcaccatcg
     1081 gttgcatacc gataggcgac ggcccgatcg aggaactctt caccctcgtg gcgaagaccg
     1141 gcgtctcgaa agtcaccgtc atcatcctgc cgaacgatgt ccgcggcggt gcgccgttca
     1201 aagcacccgc cgacccgccg aaatggctcc ccaaactgca cgacgaactc agaaaagcga
     1261 tagcgccgta taaacggtga ctctattcgc gcaagtcctt aaatatactt ccggaaccgg
     1321 accaccgaga tggcgaaggt gacgacgccg aggatagtga gcgcgacgag gtctttgtaa
     1381 agcacatcga aacccgagcc cttgaggaag agcgaacgga cgatctcaag gtagtaggtg
     1441 agcgatatca ctttcgacag atgctggaac aattcgggca tcgaatctac tgggaagacg
     1501 aaccccgaga gatagatgaa cggcatgagg atgacgaaga tcaccgtcat catcgcc
//
LOCUS       DAOQUE010000590         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_109659_length_1556_cov_3.990007, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000590 DAOQUE010000000
VERSION     DAOQUE010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109659_length_1556_cov_3.990007"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKH10_13330"
     CDS             <1..505
                     /locus_tag="PKH10_13330"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="catalyzes the formation of spermidine from
                     putrescine and S-adenosylmethioninamine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HNT29146.1"
                     /translation="RVCEKYFPATTVGLKDPRVTCHFDDGFAFMQRHENYFDVIITDS
                     SDPVGPGVALFKEDYYRLVKKALRPGGIMVSQSESPWFYEDTMSSMTAAMGQVFKHVQ
                     TYIAMIPLYPSGFWTISFASDDHRPERFDAARSAKIAANSRYYNPDIHRGALALPNFV
                     RAIVANK"
     gene            522..1451
                     /gene="mptB"
                     /locus_tag="PKH10_13335"
     CDS             522..1451
                     /gene="mptB"
                     /locus_tag="PKH10_13335"
                     /inference="COORDINATES: protein motif:HMM:NF038066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HNT29147.1"
                     /translation="MTPLHRLVHLPLVAAAVLLSALSFARWRASGTIGLDVLFPPDPA
                     AEIIGILFFVVVLLVIYGYHNAVLRCDELGLTLDRTLTLAKVLAVISLIGMPLLSNDI
                     YIYLSCGQAPLNGFNPYTDVADLATSIYHRFVPPIWAEGPYCKYGPAALSLAQFAAFV
                     GGPSPIAGLFVWKLLSLAAFFLFCDAMALLLKRFGNTAAAGPFILLSPLLWAQGVGQG
                     HNDLFAVLFLTGSLLALLYRHWILGALLLTVGASMVRDSQPGAVSGAGWGAIAMTLVA
                     LVASAVLLLVGGIAYLQSRTVSDIDPAVVLQAE"
BASE COUNT          227 a          533 c          476 g          320 t
ORIGIN      
        1 ccgcgtctgc gaaaagtatt tccccgcgac caccgtcggc ctgaaggacc cgcgcgtcac
       61 gtgccacttt gacgatggct tcgccttcat gcaacggcac gagaactatt tcgatgtcat
      121 catcaccgat tcgtccgacc cggtcggccc cggcgtggcg ctgttcaagg aggattacta
      181 ccggctggtc aaaaaggcgc tccgcccggg cggcatcatg gtgtcgcaga gcgagagccc
      241 gtggttctac gaggatacga tgagctcgat gaccgccgcg atgggacagg tcttcaagca
      301 tgtgcagacc tatatcgcga tgataccgct gtatccgtcg ggcttctgga ccatctcgtt
      361 cgcctccgac gatcaccggc cggagagatt cgacgcggcg cgcagcgcga agatcgccgc
      421 gaacagccgc tactacaatc ccgatatcca tcgcggggcg ctcgcgctcc ccaacttcgt
      481 ccgcgccatc gtggcgaaca agtgacggtc gtgccgtgaa gatgaccccg ctccaccggc
      541 tcgtccacct gccgctcgtc gcggcggccg tcctgctgtc ggcgctgtcg ttcgcccggt
      601 ggcgggcctc cgggaccatc gggctcgatg tcttgttccc gcccgacccc gccgccgaga
      661 tcatcggcat ccttttcttt gtcgtggtgt tgctcgtcat ctatgggtat cacaacgccg
      721 ttctgcggtg cgacgaattg gggctcacgc tggaccgcac cctgacgctc gccaaggtgc
      781 tcgcggtcat ttcgcttatc gggatgccgc tcctttcgaa cgacatctac atctacctct
      841 cctgcggaca ggcgcccctg aacggcttca acccctacac cgacgtggcc gatctcgcca
      901 catcgatata ccaccgcttc gtgccgccca tctgggccga ggggccctac tgcaaatacg
      961 ggcccgccgc cctgtcgctc gcgcagttcg ccgcattcgt cggcgggccg tcgcccatcg
     1021 ccgggctgtt cgtgtggaaa ctgctgtcgc tcgccgcctt tttcctgttc tgcgacgcca
     1081 tggccctgtt gctcaagcgg ttcggtaaca ccgccgccgc cggtcccttc atcctcctct
     1141 cgccactcct gtgggcgcag ggggtggggc agggacacaa cgacctgttc gcggtgctgt
     1201 tcctcaccgg ttcgttgctt gcgctcctgt accgtcactg gatactcggg gcgctcctgc
     1261 tgacggtcgg cgcgtctatg gtgcgtgact cccagccggg agccgtgagt ggagcaggct
     1321 ggggggcaat tgcgatgact ctggttgctc tggtcgcctc ggccgtcctt ctgctcgtcg
     1381 ggggaatcgc gtacttacag tcacgcaccg tatcagacat cgaccccgcg gtcgtgttgc
     1441 aggcggagta gaccgcggcc gtgcgccggc ggcataacgg cagttggtgc agatggccgc
     1501 gtcggcgaag caggtgggcg gccgcgctct gtcgtacctt tcggtacagc gcggcc
//
LOCUS       DAOQUE010000591         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_110060_length_1553_cov_3.078772, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000591 DAOQUE010000000
VERSION     DAOQUE010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110060_length_1553_cov_3.078772"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PKH10_13340"
     CDS             complement(<1..484)
                     /locus_tag="PKH10_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29148.1"
                     /translation="MSALVLALLTALKLTSYDLGDEYYLLLDTGGANISFSSFRLNEP
                     ERIVLEVSGSAAGVVLPGGGATVGRIDRNDLGERTRLVFHIAPGSAYTILHRKNLLLI
                     GFRQDLFITDETIETTLAAIETRIADRTAALAAAEAERIAAEEAKRAEEQRLAAAQAE
                     A"
     gene            complement(544..>1553)
                     /locus_tag="PKH10_13345"
     CDS             complement(544..>1553)
                     /locus_tag="PKH10_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29149.1"
                     /translation="HLGRGDQHDLHVMDVDVGDVELVPDGQGADDLVGGRDVGPLLLL
                     MAADGFVALRDRLGRIGRQALLALPIAGALFTGAVIIPQIAAQQAPDWYGELGAVKEL
                     VASRGIRDGVVFLPFRIGFGFANTLSLQDTPPHDAAGNRYLNSIPLQDAQSAAWFLAN
                     GYRSAWRLTADAGEFTIAPLEPQASDRVVIEAEYKFKPLTGSARYGFLVGNFRDEPDF
                     GFTGAVTPLSGYFGYALRFGAAGGDQYWDIEQHVMATGRYRLTVEAVATPCGGETVFS
                     VNGHRLGSLPAFSVPQRSVQLVAETALTEGVNRFTLAPLEDDRCLVIDRIVAERIDGN
                     S"
BASE COUNT          265 a          533 c          503 g          252 t
ORIGIN      
        1 tcgcctcggc ctgcgccgcc gcgagccgtt gctcctcggc ccgcttcgcc tcctccgcgg
       61 cgatccgttc cgcctcggcc gccgcgagcg ccgcagtgcg gtcggcgatc cgcgtctcta
      121 tcgcggcgag cgtcgtctct atcgtttcgt cggttatgaa caggtcctgc cggaacccga
      181 tgaggagcag gttcttgcgg tgcagtatcg tataggcgct tcccggcgcg atgtggaaca
      241 cgagccgcgt tcgttcaccg agatcgttgc ggtcgatacg gcccaccgtc gcgccgcccc
      301 cggggagcac gacccccgcc gccgaaccgc tgacctcgag cacgatgcgc tccggctcgt
      361 tcaggcggaa cgaggagaaa ctgatgttcg cgccgccggt atcgagcagg agatagtact
      421 cgtccccgag atcgtacgag gtgagtttga gcgccgtcag gagcgcgagt acgagggccg
      481 acatgtcttt tttcccctct ggtcgatcga ctatagccga aagcgggccg tttgagaaat
      541 ttttcaggaa ttcccgtcga tccgctcggc gacgatgcga tcgatgacca gacaccgatc
      601 gtcttccagc ggtgcaagcg tgaagcggtt gaccccctcg gtcaacgcgg tctcggccac
      661 caactgcacc gaccgttgcg gcaccgaaaa ggcggggagc gacccgagcc gatgcccgtt
      721 caccgaaaag acggtctcgc cgccgcacgg ggtcgcgacc gcctcgacgg tgagccgata
      781 ccggcccgtc gccatgacat gctgttcgat gtcccagtac tgatcgccgc ccgccgcacc
      841 gaacctgagc gcatatccga aataacccga cagcggcgtc accgcccccg tgaagccgaa
      901 atcgggctcg tcgcggaagt tccccacgag gaacccgtag cgcgccgacc cggtgagcgg
      961 cttgaacttg tactccgcct cgatgacgac ccggtccgat gcctgcggtt cgagcggcgc
     1021 tatcgtgaac tcaccggcgt cggcggtgag gcgccacgcc gaccggtagc cgttcgcgag
     1081 gaaccacgcg gcgctctggg cgtcctgcag cggtatagag ttgaggtagc ggttgcccgc
     1141 cgcgtcgtgt ggcggcgtat cctgcaggga aagggtgttc gcgaacccga accctatccg
     1201 gaacggaagg aacaccacgc cgtcgcggat gccccgcgag gcgaccagtt ccttcaccgc
     1261 gcccaactcg ccgtaccagt cgggcgcctg ctgggccgct atctgcggga tgatgaccgc
     1321 gccggtgaag agggcgccgg cgatgggcag ggcaaggagc gcctgtcgcc cgatccgccc
     1381 gaggcggtcg cgtagcgcga cgaacccgtc agccgccatg agaaggagga gcggcccgac
     1441 gtcgcgacca ccaacgagat catccgcgcc ctgcccgtcc ggtacgagtt cgacatcacc
     1501 gacgtcgacg tccatgacgt ggagatcatg ctggtcaccc cgtccaaggt ggt
//
LOCUS       DAOQUE010000592         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_112271_length_1534_cov_3.196755, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000592 DAOQUE010000000
VERSION     DAOQUE010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112271_length_1534_cov_3.196755"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PKH10_13350"
     CDS             complement(<1..845)
                     /locus_tag="PKH10_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29150.1"
                     /translation="MRRMLLILGAACAAGMLSAAEVTDVADSFDEDNPFDFNLNIYYK
                     NTHEFADIKREYNTLPKTYEDKDGNLYYTTESRRVETLEYKGNSETRFLRELVIAAEI
                     GLYQDLSISLDLPIVLKDAYKLILENYDIDALTGGAIPREFSLINQNLFPDAASLYYN
                     HKGVGDIAIGLQWAPFNEGRGKDEFSWLVGFKLTSPTAGIATPQGMNARVGTAIGDDI
                     QQTGKNGNVGKGLWGFDLRTAVAKRYEVAEPYFQVLASLTTPSANSIIEKSRHEFSFN
                     AGTDL"
     gene            complement(926..>1534)
                     /locus_tag="PKH10_13355"
     CDS             complement(926..>1534)
                     /locus_tag="PKH10_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29151.1"
                     /translation="SSKTTFHTLLQGTYSVRLMVKNGDNLTSTWADCDIEALPNDELI
                     VKMIWDNKDSDMDLHLVAPDGAYGDPATDCYFYNCSPQYAGARPDWGVAGETKDDPAL
                     DIDNTTGRGPETAYVNQPANGVYKVVVHAYDTSRGPSKAIVRVYTHAEQAASAEALFT
                     DTNTCWDVFSLTVTDGTGGKKNIAVQSLGTTLYPCDPPPVVP"
BASE COUNT          273 a          439 c          483 g          339 t
ORIGIN      
        1 atgaggtcgg taccggcgtt gaaggagaac tcgtgccggc tcttctcgat gatcgaattg
       61 gccgaagggg tcgtcaacga ggcgagcacc tgaaaatagg gctcggccac ctcgtagcgt
      121 ttcgcgaccg cggtgcgcag atcgaatccc cacagtccct tgccgacatt cccgttcttg
      181 ccggtctgtt ggatatcgtc gccgatggcg gtgccgacgc gtgcgttcat gccctgcggc
      241 gtcgcaattc cggcggtcgg cgaggtcaat ttgaacccga ccagccacga gaactcgtcc
      301 ttgccgcgcc cttcgttgaa tggcgcccat tgcaggccga tggcgatgtc ccccactcct
      361 ttgtggttgt agtacagaga ggcggcgtcg ggaaaaaggt tctggttgat gagcgagaat
      421 tcgcgcggga tagcgccacc ggtgagcgca tcgatgtcat agttctcgag gatcagtttg
      481 taggcgtcct tgagcactat cggcagatcg agcgatatcg aaaggtcctg atagagacct
      541 atctcggcgg cgatcaccag ttcgcgcagg aagcgcgttt cggagttccc cttgtattcg
      601 agcgtctcga cgcggcggct ttcggtcgtg taatacagat tcccgtcctt gtcctcgtag
      661 gtcttcggca gggtgttgta ttccctcttg atatcggcga attcgtgggt gttcttgtaa
      721 tagatgttca ggttgaagtc gaacggattg tcctcatcga acgaatcggc gacatcggtc
      781 acctccgccg cggaaagcat ccccgcggcg caggccgcgc cgagtatcag gagcatcctt
      841 ctcatctagc accctccggc gaacaggttc ggcggactat acgacatacg ctccggcgag
      901 tcaagaataa taacgagcgg ggctctcacg gcaccaccgg cggaggatcg cagggataca
      961 gggtcgtgcc gagcgattgg acggcgatgt tcttcttccc gccggtgccg tcggtgacgg
     1021 tcagcgagaa gacatcccaa caggtgttgg tgtcggtgaa aagcgcctcg gcgctcgccg
     1081 cctgttcggc gtgggtatag accctcacga tggccttgct gggcccgcgg ctcgtgtcgt
     1141 aggcatgcac gacgaccttg tagacaccgt tcgccggctg gttgacatag gccgtctcag
     1201 gacctctgcc ggtggtgttg tcgatgtcga gcgccgggtc gtccttcgtt tcgccagcca
     1261 cgccccaatc gggtcgcgca ccggcatact gcggcgaaca gttgtaaaaa tagcagtcgg
     1321 tcgccggatc gccgtacgcg ccgtcggggg cgaccagatg gagatccata tcgctgtcct
     1381 tgttgtccca gatcatcttg acgatgagtt cgtcgttcgg aagcgcctct atatcgcagt
     1441 cggcccaggt gctcgtcagg ttatcgccgt tcttgaccat gagccgcacg ctgtaggtgc
     1501 cctgcaggag cgtgtggaag gtcgttttcg agga
//
LOCUS       DAOQUE010000593         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_112629_length_1531_cov_4.471545, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000593 DAOQUE010000000
VERSION     DAOQUE010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112629_length_1531_cov_4.471545"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKH10_13360"
     CDS             complement(<1..338)
                     /locus_tag="PKH10_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNT29152.1"
                     /translation="MGSAIEKKAIDTIRVLAIDAIEKARSGHPGLPMGAAPMGYTLWH
                     RHLMVDPVDPSWPDRDRFVLSAGHGSMLQYALLHLAGFSLTMDDLKNFRQLGSLTPGH
                     PEFGHTAGVET"
     gene            533..1477
                     /locus_tag="PKH10_13365"
     CDS             533..1477
                     /locus_tag="PKH10_13365"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT29153.1"
                     /translation="MNASNVLRSVVVLIVVGAGSMLSAADCELCNAVEMGDDDSAKTL
                     VAQGAAVNTINDNKDTPLILAAEKGRPELVDLLLQKKADINLCDGEGESPLMRAIAMG
                     NWEIAKILIRKGANVNVADKLKRTALHYAARKENFEVFQMLLDKKAEVNAKDDEGNTA
                     LLYAVDQGNEKMMQALLKKKADVNVPGTKGKTPLMAAASSSMPGIVKVLLKAKADPNP
                     QDAYGMTALMGAASLDDVASVKELLASKADVSKKNAKGRTALMIAAEMGATEVVKMLL
                     AKGATADDKDEKGKTALDYATDEGHQKVMDILSKIPAK"
BASE COUNT          341 a          420 c          500 g          270 t
ORIGIN      
        1 gtttcgacac ccgcggtgtg gccgaactcg ggatggcccg gcgtgagcga gccgagttgc
       61 cggaagttct tgagatcgtc catcgtgagc gagaacccgg cgaggtggag cagggcgtac
      121 tgtagcatgc tgccgtgtcc cgccgagagg acgaaacggt cacgatcggg ccatgacgga
      181 tcgaccgggt cgaccatgag gtgccggtgc cagagcgtgt agcccatcgg cgcggccccc
      241 atcgggaggc cggggtgtcc cgaccgtgcc ttttctattg cgtcgatggc gagcacgcgt
      301 atcgtgtcta tcgccttttt ctcgatggct gaacccatga tatcctcccg gagtatgtcc
      361 gacgcactat aagcacggga gagagtgacg gtcaagttat ctcctcaaac agcggtattc
      421 ggtgagcgga agttatgtcc ggggcctttt tggttcgata aggttgaaaa aacttttccg
      481 tggtagtata ctgggcccgt acgaggtgct gttgcgagca aggaggtgtg ccgtgaatgc
      541 gagtaacgtc ttgagatcag tggtcgttct gatagtcgtc ggtgccggtt cgatgctgtc
      601 tgcggccgat tgcgaactct gcaacgcggt ggagatgggc gatgacgact ccgccaagac
      661 gctcgtcgcg cagggagccg cggtgaacac gatcaacgac aacaaagata cgccgctcat
      721 cctcgccgcc gagaaaggac ggccggaact ggtcgacctg ctcctgcaga aaaaagccga
      781 catcaacctc tgcgacggcg agggcgagtc gccgctcatg cgtgccatcg cgatgggcaa
      841 ctgggagatc gccaagatac tcatccgaaa gggcgccaac gtcaatgtcg ccgataaact
      901 caagcgtacg gcgctccact atgccgcgcg caaggagaat ttcgaggtgt tccagatgct
      961 tctcgacaag aaggccgagg tgaacgcgaa ggacgacgag ggcaacaccg cgctcctgta
     1021 cgcggtcgat cagggcaacg agaagatgat gcaggcgctg ctcaagaaaa aggccgatgt
     1081 caatgtgccc ggcacgaagg ggaagacgcc gctcatggcg gcggcatcga gttccatgcc
     1141 cggtatcgtc aaggtgctcc tgaaggcgaa ggccgatccg aacccgcagg atgcctatgg
     1201 catgacggcg ctcatggggg ccgcgagtct cgacgatgtc gcatcggtga aggaactgct
     1261 cgccagcaag gccgatgtgt cgaaaaagaa cgccaagggg cgcaccgcgc tcatgatagc
     1321 ggccgagatg ggggcgaccg aggtggtgaa gatgcttctc gcaaaggggg cgaccgccga
     1381 cgacaaggac gaaaaaggga agacggcgct cgactacgcg accgacgaag ggcaccagaa
     1441 ggtcatggat atcctctcga agatccctgc caagtgaccg ttccggtata tctcacataa
     1501 caaggataca cccgcactcg tcgttcttcg t
//
LOCUS       DAOQUE010000594         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_113011_length_1528_cov_3.864902, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000594 DAOQUE010000000
VERSION     DAOQUE010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113011_length_1528_cov_3.864902"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1528)
                     /locus_tag="PKH10_13370"
     CDS             complement(<1..>1528)
                     /locus_tag="PKH10_13370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29154.1"
                     /translation="HQGDTSLGVAGTTAVLGADDIPPDAFVERAILVWVVSKNPVDQN
                     AFGDNTVTLVTPDGTDHEVVAPLAGNAATPDALEFESYYKSELLGGTYYYYLYRVDVT
                     DIMKQYQEGDPESPDQLSLTGNYTVKGVDDIYDCLADANHAYCSSASMVGGWQLILVY
                     GSKQIARKRLYFYFGMDWSSGTLSNPLTIHITGFELPDQAAVKLSVVTSDGDDENSAK
                     EYLRIKGNMTQNADLTLADPCNPPDYPFNNKFLNYNYKKEPSAECIIEFSYDVDTFLL
                     QYDPNDTQGLINEHIKLGTTAFDFKIGTGNDIVLTNYMMMSVDTKLPAFDIPGMDEKY
                     LLTNSTIADRMCDDRVSAYEIWVENHGQEVASMVMVRDELPVGLKYVPGSTQIDRTGT
                     GTCYEPVEDVGGQSPLFEGMMVAETLGICQDAANCPRIMLRLKVQPLEDLPKNTSFKN
                     TALIWDAVLGQTAAYLSNQGLPLRSSLDQACPETVTDPYPPNTNCGEAPDDVPVTDAD
                     A"
BASE COUNT          246 a          421 c          528 g          333 t
ORIGIN      
        1 cgcgtcggca tcggtcaccg ggacatcgtc gggcgcctcg ccgcaattgg tattcggtgg
       61 gtaggggtcg gtcaccgttt cggggcacgc ctgatcgagc gaactgcgga gtggcagtcc
      121 ctgattgctg aggtaggcgg cggtctgccc gaggacggcg tcccagatga gggcggtgtt
      181 cttgaacgag gtgttcttgg gtaggtcctc gagcggctgg accttgagcc ggagcatgat
      241 gcgcgggcag ttcgcggcat cctgacagat gccgagcgtt tcggcgacca tcatcccttc
      301 aaagagcggc gactggccgc cgacatcctc gaccggctcg tagcaggtgc cggtgccggt
      361 gcggtctatc tgggtcgagc cggggacata cttgagcccc accggcaact cgtcgcgcac
      421 catcaccatg ctcgcgacct cctgcccgtg gttctcgacc catatctcgt aggcgctcac
      481 ccggtcgtcg cacatccggt cggcgatggt cgagttggtc agcaggtact tttcgtccat
      541 gccggggatg tcgaaggcgg gcagttttgt gtccaccgac atcatcatat agttggtcag
      601 cacgatgtcg ttgccggtcc ctatcttgaa gtcgaacgcg gtggtgccga gtttgatgtg
      661 ctcgttgatg agcccctgcg tgtcgttcgg gtcgtactgg aggaggaagg tgtcgacatc
      721 gtacgagaac tcgatgatgc actcggcgga aggttccttt ttgtagttgt agttcaggaa
      781 cttgttgttg aacgggtagt cgggcgggtt gcacgggtcg gcgagcgtca ggtcggcgtt
      841 ctgggtcata ttccccttga tgcggagata ttccttggcc gaattctcgt cgtcgccgtc
      901 gctcgttacc accgatagtt tcaccgccgc ctgatcgggc agttcgaagc cggtgatgtg
      961 tatggtgagc gggttggaga gggtgcccga actccagtcc atgccgaagt agaaatagag
     1021 ccgcttgcgc gctatctgtt tggagccgta gacgaggatg agttgccagc cgcccaccat
     1081 cgaggccgac gagcagtaag catggttggc gtcggcgagg cagtcgtaga tgtcgtccac
     1141 ccccttgacc gtatagttgc cggtcagcga cagttgatcg ggcgactccg ggtcgccctc
     1201 ctgatactgc ttcatgatgt cggtcacatc gacccggtac aggtaatagt aataggtgcc
     1261 gccgagcagt tccgatttgt agtaggactc gaactcgagc gcgtcggggg tggcggcgtt
     1321 gccggcgagc ggtgcgacga cctcgtggtc ggtcccgtcg ggggtgacga gcgtcacggt
     1381 gttgtcgcca aaggcgttct ggtcgaccgg gttcttcgag acgacccaga cgaggatggc
     1441 ccgttcgacg aaggcgtcgg gcggtatgtc gtcggcgccg agcaccgcgg tggtcccggc
     1501 gacgccgagc gaggtatcgc cctgatgg
//
LOCUS       DAOQUE010000595         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_113246_length_1526_cov_4.313392, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000595 DAOQUE010000000
VERSION     DAOQUE010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113246_length_1526_cov_4.313392"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="PKH10_13375"
     CDS             <1..1250
                     /locus_tag="PKH10_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNT29155.1"
                     /translation="SLVYDLRSGDLRVSRYWDFEIEPFEEVPHNAEAVWGEQIRALLS
                     ASVKRRLMSDVPLGVFLSGGIDSSAVTAYAVQHVGAERMKTFCIGFTEADFDESRYAS
                     RVARLFGTAHREEILSMEMAGGLVPELARRLDEPMGDASLMPTYLLARHTRREVTVAL
                     GGDGGDELFAGYDPFRALKAAALYDRAVPKPLHKAIALLAGHLPVSHRHMSLDFRIKR
                     TLMGLDHPAPLWPAVWMSTLLPDQLTELFGTPTDTEELYEEAITCWDSCRSPDIVDKV
                     LCFFTRLYLQDDILVKGDRASMMVSLEVRAPFLDIELVDLVRHIPARFKLRRGTTKYL
                     LKKALAPILPREILHRKKKGFGIPVGAWLKDRTLVVTQEEMARTGLNMSFMNRLYDDH
                     VVGRADHRLFLWNLKMLGSWLEG"
     gene            1251..>1526
                     /locus_tag="PKH10_13380"
     CDS             1251..>1526
                     /locus_tag="PKH10_13380"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNT29156.1"
                     /translation="MERFGVTKANARQFGKFLLVGLSNTGLTLAVILALLWLDTPLLV
                     ANVIGYGAGFVNSFIWNRRWTFGSTGAVWRETIVYSGVWLGSYLLQMG"
BASE COUNT          279 a          479 c          467 g          301 t
ORIGIN      
        1 gttcgctcgt ctacgacctg cgtagcggcg acctgcgcgt cagccgctac tgggacttcg
       61 agatcgagcc gttcgaggag gtgccccata acgccgaagc ggtgtggggc gagcagatcc
      121 gcgcgttact ttccgcatcg gtgaagcgac ggctcatgtc cgatgtgccg ctcggggtgt
      181 tcctgagcgg cgggatagac tcatcggcgg tgaccgccta cgcggtccag catgtcggcg
      241 cggaacggat gaagactttc tgcatcggct ttaccgaggc cgattttgac gagtcacgct
      301 atgcgtcacg ggtagcaaga cttttcggga cggcgcatcg cgaagagata ctctcgatgg
      361 agatggccgg gggactcgta cccgaactcg cgcggcgtct cgacgagccg atgggcgacg
      421 cctcgcttat gcccacctat ctgctcgcgc gccacacccg gcgcgaagtg accgtcgcgc
      481 tcggcggcga cggcggcgac gaactgttcg ccggttatga cccgttccgg gcgctcaagg
      541 cggcggccct gtacgaccgc gcggtgccca aacctctgca caaggcgata gcgctcctcg
      601 ccggtcatct gccggtatcg caccgccaca tgagtctcga cttcaggata aagcggacgc
      661 tcatggggct cgatcacccg gccccgctct ggcccgccgt ctggatgtcg accctgctcc
      721 ccgaccagtt gaccgaacta ttcggcacac cgaccgatac cgaggaactc tacgaagagg
      781 cgatcacctg ttgggactcc tgccgatcgc ccgacatcgt tgacaaggtg ttgtgcttct
      841 tcacgagact ttacctacag gacgacatcc tcgtgaaggg cgatcgggcg agcatgatgg
      901 tatcgctcga ggtgcgcgca ccgttcctcg acatcgaact ggtcgatctc gtccgccaca
      961 taccggcgcg attcaaattg cggcgcggca cgacgaaata tcttctcaaa aaagctctcg
     1021 ctccgatcct gccgcgcgag atacttcacc ggaaaaagaa aggtttcggc ataccggtcg
     1081 gcgcatggct caaagatcgg accttggtgg tgactcagga ggagatggca cggacaggtc
     1141 tcaacatgtc gttcatgaac cgtctgtatg acgaccacgt ggtcggacgt gccgaccatc
     1201 ggctcttcct gtggaacctc aaaatgctcg gcagttggct ggagggctag atggaacgat
     1261 tcggcgtgac caaggcgaac gcccggcaat tcggcaagtt cctgctcgtc gggctttcga
     1321 ataccgggct cacccttgcg gtcatcctcg cactgctctg gctcgacact ccgctccttg
     1381 tcgccaatgt catcgggtac ggcgccggct tcgtgaacag tttcatctgg aaccgccgct
     1441 ggaccttcgg atcgaccggc gcggtatggc gcgagacgat cgtctattcc ggcgtctggc
     1501 tcgggtccta tctcctgcag atgggc
//
LOCUS       DAOQUE010000596         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_113263_length_1526_cov_3.254929, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000596 DAOQUE010000000
VERSION     DAOQUE010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113263_length_1526_cov_3.254929"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PKH10_13385"
     CDS             <1..524
                     /locus_tag="PKH10_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HNT29157.1"
                     /translation="CSKTLYDDIKTRGGKGIMWKAGHSLIKAKMKEVGAAMGGEMSGH
                     IFFKHRFFGYDCAIYSMFRLLEILSERDDLDVRRLLDGIPKTVSTPEIRFEVDEEKKF
                     PVVAKTVAHFKEQGYDVNDIDGARITFADGWGLLRPSNTQNVIVMRFEAETDKRLKEI
                     QALIEGTLNEYLK"
     gene            535..>1526
                     /locus_tag="PKH10_13390"
     CDS             535..>1526
                     /locus_tag="PKH10_13390"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNT29158.1"
                     /translation="MSKTLVMVLAGGQGSRLMPLTTERAKPAVPFGGVYRIIDFVLSN
                     FVNSGYMQIKVLTQFMSNSLNQHIARNWGLSMQFNQYVETVPAQQRTGDNWYLGSADA
                     IFQNLNILRDERPEHVFVFSGDHIYKMDVSQMFNFHLHKDADMTIAAIPVPLKDASQF
                     GVIEVDEDWRMIGFEEKPKEPKGIPGRPDLALASMGNYIFKRDTLAQAVSEDAEKKES
                     AHDFGKNVIPALYPRKKVYIYDYLSNKIRLPDSDQYEKPYWRDVGTIWSYWESHMELV
                     SVSPAVNLYNRNWPIFGFPDVNMPSAKFVFADQANQRVGLATDSMVSVGCIISGG"
BASE COUNT          350 a          446 c          447 g          283 t
ORIGIN      
        1 aatgcagtaa gacgctctac gacgacatca agacgcgcgg cggcaagggc atcatgtgga
       61 aggcgggcca ttcgctcatc aaggcgaaga tgaaggaggt cggtgcggcg atgggcggcg
      121 agatgagcgg ccacatcttc ttcaaacacc gcttcttcgg ttacgactgc gcgatataca
      181 gcatgttcag gctcctcgag atactttccg aacgcgatga tctcgatgtc cgccgtctgc
      241 tcgacggcat ccccaagacg gtgagcaccc ccgagatccg gttcgaggtc gacgaggaga
      301 agaaattccc ggtggtggcg aaaacggtcg cgcatttcaa ggaacagggc tacgatgtca
      361 acgacatcga cggcgcccgc atcaccttcg ccgacggctg gggtctgttg cgcccctcca
      421 acacgcagaa tgtcatcgtg atgcggttcg aggccgagac cgataagcgc ctgaaggaga
      481 tacaggcgct catcgaaggg acgctcaacg aatatctgaa gtaggggaag cgatatgtcc
      541 aaaacgctgg tcatggtgct tgcgggcggt cagggaagcc gcctgatgcc gctcaccacc
      601 gaacgggcga aaccggcggt gccgttcggt ggcgtctacc gcatcatcga tttcgtcctg
      661 tcgaacttcg tcaacagcgg ctacatgcag atcaaggtgc tgacgcagtt catgtcgaac
      721 tcgctcaacc agcacatcgc gcgcaactgg ggactttcca tgcagttcaa ccagtatgtc
      781 gagacggtcc cggcgcaaca gcggaccggc gataactggt atctcgggag cgccgacgcg
      841 atattccaga acctcaacat cctgcgcgat gaacggcccg aacatgtgtt cgtcttcagc
      901 ggcgaccata tctacaaaat ggatgtttcg cagatgttca acttccatct gcacaaggat
      961 gccgacatga cgatagcggc gataccggta ccgctcaagg atgcttcgca gttcggcgtc
     1021 atcgaggtcg acgaggactg gcgcatgata gggttcgagg agaagccgaa ggaacccaaa
     1081 ggcatccccg gtcgccccga cctcgcgctt gccagcatgg ggaactacat cttcaagcgc
     1141 gacactctcg cgcaggccgt gtcggaggat gccgagaaaa aagaaagcgc ccacgatttc
     1201 ggcaagaatg tcatcccggc gctctacccc cgcaagaagg tctacatcta cgactacctt
     1261 tcgaacaaga tacgcctgcc cgacagcgac cagtacgaga aaccctactg gcgcgatgtc
     1321 ggtactatct ggagttactg ggagagccac atggaactcg tgtcggtcag cccggcggtc
     1381 aacctgtaca accgtaactg gcccattttc gggttccccg atgtcaacat gccgtcggcc
     1441 aagttcgttt tcgccgatca ggcgaatcag cgcgtcggtc tggccaccga ctcgatggtc
     1501 tcggtcgggt gcatcatatc gggcgg
//
LOCUS       DAOQUE010000597         1525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_113369_length_1525_cov_4.374830, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000597 DAOQUE010000000
VERSION     DAOQUE010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113369_length_1525_cov_4.374830"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1084
                     /gene="trxB"
                     /locus_tag="PKH10_13395"
     CDS             167..1084
                     /gene="trxB"
                     /locus_tag="PKH10_13395"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNT29159.1"
                     /translation="MSQRYDVVIVGGGPAGLTAAIYLGRAMLSTIIVEKGAIGGLMAL
                     TDKLENYPGHETVSGGDLSEVMLKQATRFGAQMLMGTVTKVEKTADGFTVTSDGGLVS
                     ARSLIWAAGSSPRRIDVPGEMDFVGRGVSYCAVCDGAFYRNLRVAVVGGGDSSLKEAL
                     YLTRFASEVVIIHRRAEFRAERIIQEEVRKHPKITLMLDSVVEKIMGKDFVESVTVKN
                     VKSGETSDHPFDGVFVFVGYTPQTEPIKHLVTLSPSGRIQLRPDGTTGTPGLFAAGDV
                     VEKVVYQVATAVGDGAAAATATERYLSGH"
BASE COUNT          333 a          397 c          485 g          310 t
ORIGIN      
        1 ggaacctctt ctgggagatc tatgcgcggg tcgagggagg cgccgcctac gatggcgatc
       61 cgagccccgt cgccatcagc atcgtgcgca ggccgcaggt gaccgccgtc ggtggtgcgt
      121 cgttcggagg gaagttctag gaacatgaaa catacgggag atcgacatgt cacagaggta
      181 tgatgtcgtt atcgtcggtg gcgggccggc cgggctcacc gccgccatct atctgggccg
      241 cgcgatgctc tcgaccatca tcgtcgagaa gggggccatc ggcgggctca tggcgcttac
      301 cgacaaactg gagaactatc cggggcacga aacggtgagc ggcggcgatc tgtcggaagt
      361 gatgctcaag caggcgaccc gcttcggcgc gcagatgctg atggggacgg tgacgaaggt
      421 cgaaaagacg gcggacggct tcaccgtaac ctcagacggt ggcctggtgt cggcgcggag
      481 tctcatctgg gccgccggtt cgtctccgcg caggatagat gtgccgggcg agatggattt
      541 cgttggccgc ggcgtttcct actgcgcggt gtgcgacgga gcgttctatc gcaacttgcg
      601 ggtggcggtg gtcgggggcg gcgattcgtc tctcaaggag gcgctctacc tcacgcggtt
      661 cgcctcggag gtggtcatta ttcaccggcg tgccgaattc cgcgccgaga ggatcattca
      721 ggaagaggta cgcaagcatc ccaagataac actgatgctc gacagcgtcg tcgagaagat
      781 aatgggcaag gatttcgtcg aatcggtcac cgtgaagaat gtcaaaagcg gcgaaacgag
      841 cgaccacccg ttcgacggcg tctttgtctt tgtcggctat acaccgcaga cagaaccgat
      901 caaacacctc gtgacgctgt caccaagcgg gcgtatccag ttacgacccg acggcacgac
      961 cggcactccg gggcttttcg cggcgggcga tgtggtcgaa aaggtcgtct atcaggtcgc
     1021 gacggccgtt ggcgacggcg cggcggcggc gaccgccacg gaacggtatc tgtcgggtca
     1081 ctgattattc ctgtcaaggg ggatcgaggg ggtttgtccc attccggcga gatgaaaaac
     1141 ccaccctgcc ctcccttgac agggcgggaa ggcggtcgtc ttcctgtgaa ggatgatcga
     1201 gggggtttgt gcaaacctcc cttttcttgt cttttcatct ccgcttgtta taatgcgctg
     1261 tttttaacgg gacaaatgat ggacttcagc gcatacggac gcggttttgc ggcacatgaa
     1321 tggcagactg tctctaaaac cattcacaaa aatttggtaa cggatattat caaccagtga
     1381 agggagtatc ccatgagaga aaaaaaggtg acagtgcagg aaacaatgtt tggacagaaa
     1441 aacaagaaga tgaaaactac atttattgta gtgttgaagc aaagtcaaaa tgtcccccta
     1501 actgcaaagc gattatgtcc cctag
//
LOCUS       DAOQUE010000598         1525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_113431_length_1525_cov_2.453061, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000598 DAOQUE010000000
VERSION     DAOQUE010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113431_length_1525_cov_2.453061"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="PKH10_13400"
     CDS             complement(<1..1504)
                     /locus_tag="PKH10_13400"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HNT29160.1"
                     /translation="MHHTIATSVYKKLPYDFQKDFAPLTVIAMVPNVLAVSAASPAKT
                     VADLVAMAKTAKPELSYGSNGNGTAQHLIDIGPGSGPDGGTVVASGAPVEVMKHPESL
                     TAKALREFSDGWKDIALKERRIVTVDTPLTLTGVTTNNLRDITVSFPERRWTMVTGRS
                     GSGKSSLVFDTLYALGRNSYLETFPSYLRSRLEMPGGAQAASWSGLLPAVGIGAHDGD
                     RGHPRSTVGTVTDIQDHYRLLFARSAGAGWRAAQFSPNHEEGACPVCRGLGFRRVCAP
                     EKLITHPDRSLLAGALDGTPTGRSYGEPDGKTVATLRAMCAVHGIDLSVPYREFPDAA
                     RQLILHGTGDTVYPVAWHYKRGVRTGTHQFTSVWKGFLPLVEEEYLLRHDNTRGKTIE
                     MVMSDIPCAACDASGLNDRARSVTVFGTTIDKLSALPAAAALSFFNGLAAMSPEEREI
                     VTALIPRLEALIRVGLGYLSPDRRTLTLSGGEYRRLHLAAALFTGLTGVCY"
BASE COUNT          242 a          486 c          537 g          260 t
ORIGIN      
        1 cataacagac gccggtgagt ccggtgaaga gcgccgccgc gaggtggagc cgccggtatt
       61 cgccgcccga aagggtgagc gtccggcggt cgggcgacag gtagccgagc ccgacgcgga
      121 tgagcgcttc gagccgcggg atgagcgccg tcacgatctc gcgttcctcg gggctcatag
      181 cggccaaccc gttgaagaac gagagcgcgg cggcggcggg aagcgccgaa agtttgtcta
      241 tcgtggtgcc gaacaccgtg accgaccggg cgcggtcgtt gaggccgctt gcatcgcagg
      301 cggcgcaggg gatatcgctc atcaccatct cgatcgtttt gccgcgggtg ttgtcatggc
      361 gcagcaggta ttcctcttcg accagcggca ggaacccctt ccagacgctc gtgaactggt
      421 gggtgccggt ccggacgccg cgcttgtagt gccacgccac gggatagacc gtgtcgccgg
      481 tcccgtgcag aatgagttgg cgcgccgcgt cgggaaactc gcggtagggt accgaaaggt
      541 cgatgccgtg caccgcgcac atcgcccgca gtgtcgcgac cgtcttcccg tcgggttcgc
      601 cgtaggaacg gccggtcggc gtaccgtcga gcgccccggc gaggagcgat cggtcgggat
      661 gcgttataag tttttcgggg gcgcagaccc gtctgaagcc gagcccgcgg cagacggggc
      721 aggccccttc ctcgtggttg ggcgagaact gcgccgcacg ccaccccgcc ccggcgctcc
      781 gcgcgaaaag gagccgatag tggtcctgaa tatcggtcac ggtgccgacg gtcgagcgcg
      841 ggtgtccccg gtcaccgtcg tgcgccccga tgccgacggc ggggagaagt ccgctccacg
      901 acgccgcctg cgcaccgccc ggcatctcga ggcgactccg caggtaactc gggaatgtct
      961 cgaggtaact gttgcggccg agggcgtaga gcgtgtcgaa gaccagcgac gacttccccg
     1021 aaccggagcg gccggtgacc atcgtccagc gccgttcggg gaacgacacg gtgatgtcgc
     1081 gcaggttgtt ggtcgtgacg ccggtgaggg tgagcggcgt atcgaccgtg acaatgcgcc
     1141 gttctttcag cgcgatatcc ttccagccgt cagagaattc acgcagtgcc ttcgcggtga
     1201 ggctttcggg gtgtttcatc acctcgaccg gcgcgcccga tgcgaccacc gtgccgccgt
     1261 cgggaccgct cccgggaccg atgtctatca ggtgttgggc cgtgccgttt ccattggagc
     1321 cgtaactcag ctcgggcttg gccgttttgg ccatcgcaac gaggtccgcc acagtcttgg
     1381 ccggcgaggc cgcactgacg gccagcacgt tgggcaccat cgcgatcacc gtcagcggcg
     1441 cgaagtcctt ctggaagtcg tacggcagct tcttgtagac gctggtcgcg atcgtgtggt
     1501 gcaccgcgcc aacgagcagc gtgta
//
LOCUS       DAOQUE010000599         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_113756_length_1522_cov_3.670757, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000599 DAOQUE010000000
VERSION     DAOQUE010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113756_length_1522_cov_3.670757"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKH10_13405"
     CDS             complement(<1..262)
                     /locus_tag="PKH10_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNT29161.1"
                     /translation="MTMTSNSFDNLNLIPQIRKAVAEEGYETPTPIQSRAIPVVLDGK
                     DLFGIAQTGTGKTAAFAIPILQRLSIEGGHERGRRAIKALICT"
     gene            complement(390..1487)
                     /locus_tag="PKH10_13410"
     CDS             complement(390..1487)
                     /locus_tag="PKH10_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29162.1"
                     /translation="MKFVFIGFFAVLIFAACDNTTANRADADTVTDQTVAGDADEIVN
                     DQPDQSETDQTDDVIPDNLVTDDTVDEIPDDDTVGTDCMTNDQCAEDEFCAKPLGSCS
                     EAVAGKCDGRPTDCFMTRAVAPQCGCDDYTYSNQCWAAASGAVIAHAGECDGDVLCFR
                     NDECGENEFCQKKTGVCDMGFGVCRPQPTDCPQPEEPAPVCGCDLREYGDECTAWMND
                     IPVKHEGACTGPKDSMVRYFYADDAETPEGYLRIVVSPTNIREFWTTDTFTEQFNGNS
                     SVTLTVRFYESDLPESDYAELQYTLSLPPTLPMGVGFGYEGSYLRWYSFNGVALGDMQ
                     GTVITYEYVRHDAGNEVSTLIVSGLNLWVGE"
BASE COUNT          289 a          458 c          461 g          314 t
ORIGIN      
        1 gggtgcagat gagcgccttg atggcgcggc ggccccgttc atgccctcct tcgatggaga
       61 gacgctggag aatggggatg gcgaacgcgg cggtcttgcc ggtgccggtc tgggcgatgc
      121 cgaacaggtc cttgccgtcg agcaccaccg ggatggcacg cgactggatg ggggtcgggg
      181 tctcgtagcc ctcctcggcg accgccttgc ggatctgcgg gatgaggttc agattatcga
      241 acgaattcga tgtcatggtc atgtaacaat actccaaaaa tcgtttttca aattaccgat
      301 cgatatggct ggctgcagac ggaatgcaga agcgtcgaac gatcacgggc cttttatagc
      361 acaaaagcgg gaaatagcaa gagttatttt cactctccca cccagaggtt gagcccgctg
      421 acgatgagcg tcgagacctc gttgccggcg tcgtgccgca catattcgta ggtgatgacg
      481 gtcccctgca tgtcccccaa tgccactccg ttgaaggaat accatctgag gtaacttccc
      541 tcgtagccga acccgacgcc catcgggagc gtcggcggga gcgagagggt gtattgcaac
      601 tcggcgtagt ccgactcggg gaggtcgctc tcatagaacc tgacggtaag cgtcaccgaa
      661 ctgttcccgt tgaactgctc ggtgaaggta tcggtcgtcc agaactcgcg gatgttggtc
      721 ggcgacacga cgatccgcag gtagccctcg ggcgtctcgg catcgtcggc gtagaagtaa
      781 cggaccatcg aatctttggg accggtgcat gcgccttcat gcttgaccgg gatatcattc
      841 atccacgcgg tgcactcgtc gccatactcc ctgaggtcgc aaccgcagac cggcgccggt
      901 tcttccggct ggggacagtc ggtcggctgc ggacggcaga cgccgaaccc catgtcgcag
      961 acgccggtct tcttctgaca gaactcgttc tcgccgcact catcgttgcg gaagcacagc
     1021 acatcgccgt cgcattcgcc cgcatgcgct atcaccgcgc cgctcgcggc ggcccagcac
     1081 tggttgctgt aggtgtagtc gtcacagccg cattgcgggg cgacggcgcg cgtcatgaaa
     1141 cagtcggtcg gccgaccgtc gcatttcccc gcgaccgcct cactgcacga gccgagcggc
     1201 ttggcgcaga attcatcctc ggcgcactga tcgttggtca tgcaatcggt gcccacggtg
     1261 tcgtcatcgg gaatttcgtc gacggtatcg tcggtgacca agttgtcggg gatgacatcg
     1321 tcggtttgat cagtctccga ctgatcgggc tgatcattga cgatctcgtc cgcgtcgccc
     1381 gccaccgtct ggtcggtcac ggtgtcggcg tcggccctgt tcgccgtcgt gttgtcgcac
     1441 gccgcgaaga tcaggacagc gaaaaaccca ataaaaacga acttcatgat atctcctccc
     1501 tcaaagacaa caccctttag tg
//
LOCUS       DAOQUE010000600         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_114002_length_1520_cov_3.920137, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000600 DAOQUE010000000
VERSION     DAOQUE010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114002_length_1520_cov_3.920137"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PKH10_13415"
     CDS             complement(<1..359)
                     /locus_tag="PKH10_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29163.1"
                     /translation="MRDPSHPLKLLLILGVLLLCSPAPAEEAIVVWRIEPKTGVTDKD
                     ADAISAMVTTEVGRVSGRQTIGETDMKALMVGEEKKLSCGAEDTACVAEIGAALGAPE
                     SVTGTLSGSALASPVQLA"
     gene            520..948
                     /locus_tag="PKH10_13420"
     CDS             520..948
                     /locus_tag="PKH10_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT29164.1"
                     /translation="MQEGYLVRDYRSGDWTAVEALWTATGMGGAYRGDTAEVVRRTLD
                     VGARLLVMERRDTGEVVGTAWLTHDTRRSYLHHFGILPAYQGKKLANDLMAATLKAAR
                     EMGFQCKLEVHRENVAAVTLYQKIGFKRLGDYDVYIMRDL"
     gene            1044..>1520
                     /locus_tag="PKH10_13425"
     CDS             1044..>1520
                     /locus_tag="PKH10_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29165.1"
                     /translation="MFGRRPLRLLFLFFSSAILFAACSSEEPETISCVTAFDCPIDWV
                     CEDNVCVEFPDSETPLDKDTFYKDADNGGDADGPTDLDGVTDTAGDADRGDDDSVTDA
                     DATGDQDILTDDGGVGDGDDLPGDADDIASDDDTLLVEEDHAQADDGTATTDDGTIA"
BASE COUNT          279 a          446 c          490 g          305 t
ORIGIN      
        1 gcgagttgca ccgggctggc gagcgccgag ccggagaggg tgccggtcac cgactcgggt
       61 gccccgagcg ccgcgccgat ctcggcgacg caggcggtgt cctcggcgcc gcaggagagt
      121 tttttctctt cgccgaccat gagcgccttc atgtcggtct cgccgatggt ctgtctgccc
      181 gaaacgcggc ccacctcggt cgtcaccatc gcgcttatcg cgtcggcgtc cttgtcggtg
      241 acccccgtct tcggctcgat gcgccacacc acgatggcct cttcggccgg ggcgggcgag
      301 cagagaagaa gaacgccgag gatcagcaga agtttcaggg ggtgggacgg gtcgcgcatg
      361 gcttttcctc cgttccgggt caaagatgga ccgtatcggg gtcgcagtat atccctcagc
      421 ccccgttttc tcaaatttta ttatcgggac gccgttctcc ttcgatttga caacggtcac
      481 cgcccgggta cactagcccg ggtcctatcg gaggtgacca tgcaggaagg atatcttgtc
      541 cgtgactacc ggtccggcga ttggacggcc gtcgaggcgc tctggacggc gaccgggatg
      601 gggggcgcct atcgcggcga caccgccgaa gtggtgcggc gaacgctcga cgtcggggca
      661 cgtctattgg tgatggaacg gcgcgacacc ggcgaggtgg tcggcaccgc atggctgacc
      721 cacgataccc ggcgcagtta tctccaccat ttcgggatac ttccggccta tcaggggaag
      781 aaactcgcca acgatctcat ggcggcgacg ctcaaggcgg cgcgcgagat gggattccaa
      841 tgcaaactcg aggtgcaccg cgagaatgtc gcggcggtga ccctttatca aaagatcgga
      901 ttcaagcgtc tgggcgatta cgatgtctat atcatgcggg acctctgatc catagttctt
      961 aaaaatatcc tcgtcagggg ttgctttttc gacccccgtg cgctaaaatc ggcccgtgtt
     1021 gttttatcgt tagggaaaag ggcatgttcg gcaggcgacc gttgcggctc ctctttttgt
     1081 tcttttcctc ggctatcttg tttgccgcct gctcttccga ggaacccgag accatcagtt
     1141 gcgtcacggc cttcgactgt cccatcgatt gggtctgtga agataatgta tgcgtcgagt
     1201 ttcccgacag cgagacgccg ctcgacaagg atacctttta caaggatgcg gataacggcg
     1261 gcgatgccga cggtccgacc gacctcgacg gcgttaccga caccgccggc gatgccgacc
     1321 ggggggatga cgacagcgtg accgatgccg atgctacggg cgatcaggac atcctgaccg
     1381 atgatggcgg ggtaggggat ggcgatgacc tgccgggaga tgccgacgat atcgcgagcg
     1441 acgatgatac cctcctcgtg gaagaggatc acgcgcaggc ggacgacggt acggcgacaa
     1501 cggatgatgg cacgatagcg
//
LOCUS       DAOQUE010000601         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_114099_length_1519_cov_4.291667, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000601 DAOQUE010000000
VERSION     DAOQUE010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114099_length_1519_cov_4.291667"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="PKH10_13430"
     CDS             complement(<1..1293)
                     /locus_tag="PKH10_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29166.1"
                     /translation="MNSAVLRSSIVPGLLLSLLLCVSCKKDEDKGPIDTGGSDNSTSD
                     SDTIATGGCGDGVINGDEVCDGPVDCSTQGRYHIGQLATCNADCSAYDLSNCIERDPG
                     DLCGNGVVEPSQNYEVCEIGDTKPCTEENPNFREGDLATCADNCRTWDTYDCVLAGTD
                     TCAQIYDCVAGCGDITCETDCLDAGSDDGKARYEALMACFEQNCASGADVKECTETAC
                     PTDYFTCFPSEKCGNGTIDEGEVCEKGDTKDCGELDPEKYKPGKEALCNSICTAFDVY
                     MCIDINALTCYEVYTCIGECAGDTTCEDACKAQSYADANTRLDTMLTCYEEECANTDN
                     GCYAEKCEFQTDACKTHATCGDKVIDQYEICESGDKKDCGEVDPDKYEAGTANAFCNV
                     NCTRWSTTGCFGFCSCGEVKLCVDQCPGGQATTDSECVN"
BASE COUNT          265 a          454 c          464 g          336 t
ORIGIN      
        1 gttcacgcat tcgctgtcgg tggtcgcctg accacccggg cactgatcga cgcagagttt
       61 gacctcgccg caggagcaga agccgaaaca gccggtcgtg ctccagcggg tgcagttgac
      121 attgcagaag gcgttggcgg tccccgcctc gtacttgtcg ggatcgacct cgccgcagtc
      181 cttcttgtcg cccgactcgc agatctcgta ctggtctatc accttgtcgc cgcaggtcgc
      241 gtgggtcttg caggcgtcgg tctggaactc gcatttctcg gcgtaacaac cgttgtcggt
      301 gttggcgcac tcttcctcgt agcaggtgag catcgtgtcg agccgcgtgt tggcatcggc
      361 gtaactctgc gccttgcagg cgtcctcaca ggtcgtgtcg ccggcgcatt caccgatgca
      421 ggtatagacc tcgtagcagg tcagcgcgtt gatgtcgatg cacatataga catcgaacgc
      481 ggtgcagatg gaattgcaga gcgcctcctt gcccggttta tatttctccg ggtcgagttc
      541 gccgcagtct ttggtatcgc ccttctcaca gacctccccc tcgtctatcg tgccgttgcc
      601 gcatttttcg gaggggaaac aggtgaaata atcggtcgga caggccgttt cggtgcattc
      661 cttgacatcg gcgccgctcg cgcagttctg ctcgaaacag gccatgagcg cctcgtaccg
      721 cgccttgcca tcgtccgatc cggcgtcaag gcagtccgtc tcgcaggtga tgtcgccgca
      781 ccccgcgacg cagtcgtata tctgggcgca ggtatcggtg ccggcgagca cgcagtcgta
      841 ggtgtcccag gtgcggcagt tatcggcgca ggtcgcaagg tcgccctcgc ggaaattggg
      901 gttctcttcg gtgcagggct tggtgtcgcc gatctcgcag acctcatagt tctggctcgg
      961 ctcgacgacg ccgttgccgc acagatcgcc cgggtcgcgc tcgatgcagt tgctcaggtc
     1021 gtaggcggaa caatcggcat tgcaggtggc caactgaccg atatggtagc ggccttgcgt
     1081 agaacagtcg accggaccgt cgcagacctc gtcaccgttt atgacgccgt cgccacagcc
     1141 accggtcgcg atggtgtcac tgtcggaggt ggagttgtcg ctaccgccgg tgtctatcgg
     1201 ccccttgtcc tcgtcctttt tgcacgacac gcacagcaac agtgacagaa gaaggcccgg
     1261 aacgatactg gagcgcaaaa cggctgaatt catctcacat tctcccttga cgatgtcttt
     1321 tttcaactcg cgggaacgag tgtagccgac aaaaaatgat ttgtcaatag cgcggacggt
     1381 cgctcccggg aagcaggtta ttggctttgc gccgccggaa cgatgaccgt cacgaggcgg
     1441 gaggagagat aggcaccgtt gccaagcacc gtcacgagcg cctgataggt catcggattg
     1501 gtcgtcgtgt tcttgcaga
//
LOCUS       DAOQUE010000602         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_114822_length_1513_cov_4.083676, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000602 DAOQUE010000000
VERSION     DAOQUE010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114822_length_1513_cov_4.083676"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKH10_13435"
     CDS             complement(<1..310)
                     /locus_tag="PKH10_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29167.1"
                     /translation="MRRFVVFLIFFPLLFLLSCAGEKSAVAEADTVSDAVPDTAPDAA
                     TDTLTTQDDTVPTVDDILPEADLLDTDESAAPDDDILLTCDDLWMGGAVLVVTSKDTS
                     I"
     gene            complement(337..1299)
                     /locus_tag="PKH10_13440"
     CDS             complement(337..1299)
                     /locus_tag="PKH10_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29168.1"
                     /translation="MRGSFMFLILCSIFFVGCGGVNCEPCEKEATCDDTVHPDGENLL
                     PEADELLSDADEPTVVLESFIPLMEDDGCEVAPDSHQWYSRGYLDVAFATQYRLPFRT
                     IDSLPAEPWQLTRMTFSYELPPIPGVDPAVWDDRTVHSFPRAESDGAVTVATVLLVTD
                     EIYKNLIEIFDIFASSGVSFDWESYPVVVTVQAAGVRPGNETLIWTGELIFELVPLYA
                     QSIRSAAIYPDKAWPTDQKIEAEYPPTEWAEKKYERDKDIYDTMLLNCDFSEDHLIGC
                     LPGQDYGLIDCYTYTEAATTIETLFPGYLCCPKDEPVAPVEPNI"
BASE COUNT          327 a          461 c          396 g          329 t
ORIGIN      
        1 cgatggaagt atcttttgat gtcaccacca gcaccgcccc gcccatccac aagtcgtcgc
       61 aggtcaggag gatatcgtcg tcgggcgccg cactctcgtc tgtgtctaaa agatcggcct
      121 ccggcaggat atcgtcgaca gttggtaccg tgtcgtcctg cgtggtcagc gtgtcagtcg
      181 ccgcgtcggg tgccgtgtcg ggcaccgcgt cagacaccgt gtcggcctca gccaccgcac
      241 ttttttctcc agcgcaggaa aggagaaaaa gaagcgggaa aaatatgagg aacacgacga
      301 atcgtctcac ggcaccatcc acccgcttac ctaaaattat atattcggct ccaccggcgc
      361 taccggctca tctttcggac agcagagata tccggggaag agcgtttcta tcgtcgtcgc
      421 cgcttcggtg taggtataac agtctatcaa accatagtcc tgtcccggca ggcaaccgat
      481 caaatggtct tccgagaaat cgcaattcaa tagcatggta tcgtagatgt ccttgtcgcg
      541 ctcgtacttc ttctcggccc attctgttgg tgggtactcc gcctctatct tctgatcggt
      601 cggccacgct ttgtctggat agatagcggc gctacggatc gactgggcgt aaagcggtac
      661 aagttcaaag atcagttcgc cggtccagat gagcgtttcg ttccccggcc gcactccggc
      721 cgcttgaacg gtgacaacga cagggtagct ctcccaatca aacgacactc cgctactggc
      781 aaagatgtcg aatatctcta tcagattctt gtatatctcg tcggtgacca gcaacaccgt
      841 ggccaccgtc accgcaccgt cgctctcggc ccggggaaac gaatggaccg tgcggtcatc
      901 ccacacggcg ggatccactc cggggatcgg cggcagttca taggaaaagg tcatccgcgt
      961 cagttgccac ggctctgccg gtagcgaatc gatcgttcgg aagggaagac ggtattgggt
     1021 ggcgaaagcg acatcgagat aaccccggct ataccattgg tgcgaatcgg gcgccacttc
     1081 gcacccgtca tcttccatga gcgggatgaa actttccaac acaaccgtcg gctcgtcggc
     1141 gtcggaaagc agttcgtcgg cctcgggaag caggttttca ccgtcgggat gtacggtatc
     1201 atcgcatgtt gcttcttttt cgcagggctc gcagttcact cctccacatc ccacgaagaa
     1261 gatcgagcaa agaataagga acatgaatga tcctctcata ccacctccac ccgagcacct
     1321 tatccacaca tacgctactt gaacagcccc ttgacgaagg gcgacttgaa ctcgcggtcg
     1381 aggatgtcca tgcagacctc gtcccagaac tttcccgcta tcaatttcgc ctcgcgccgc
     1441 cgccccacct ccttgaaccc gcatttgcgg taacacccca gcccccgttc gttgtaggca
     1501 taggcgttga ggt
//
LOCUS       DAOQUE010000603         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_115130_length_1511_cov_2.439560, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000603 DAOQUE010000000
VERSION     DAOQUE010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115130_length_1511_cov_2.439560"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PKH10_13445"
     CDS             complement(<1..1206)
                     /locus_tag="PKH10_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29169.1"
                     /translation="MCSLRHPLMGILVLSFLFLAACDGTKTTKSDADQLPTDDIAVDG
                     DLLTDDLIPDEEEPDDADIDTKTDTKPDGDKLDGDMPDTDTVDVEPDIDEPVTDDMVT
                     DDVVTDDVVTDDVVVDDVVTDDILTDEDTVPVTRRTLCLNIPENAHGIGANADGKFEQ
                     TQNGAVWEPETFECAWECNKSYLPNEAGDACFFLATIYVNHAATDGANNGSSWENAFT
                     DLSDAIEEALDNSQQEIWVAQGTYTPTFCFTSPQETCPFLPRKQTFTLKPGVGIYGGF
                     VGDGTARDERDWEANETILSGDLDGDDAWDDLNEEWLNRDENAYHVIQIIWDDRHQAD
                     AVLDGFTVTGGVAIDEDDEGIEDKGGGLLAGDNEDSADFTVRNCRFVANTTAGGGGAI
                     AVLEMSPTIE"
     gene            complement(1301..>1511)
                     /locus_tag="PKH10_13450"
     CDS             complement(1301..>1511)
                     /locus_tag="PKH10_13450"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNT29170.1"
                     /translation="FGTDNGNNIDLDPLFVGSGADPLDLQATSPCIDAGSNALVPEGI
                     LYDILGDARIQTGPAGVVDMGAYEQ"
BASE COUNT          268 a          473 c          455 g          315 t
ORIGIN      
        1 ctcgatggtc ggggacatct ccagcacggc gatggcaccg ccgcccccgg cggtggtgtt
       61 ggcgacgaac cggcagttac gcaccgtgaa gtcggccgaa tcctcgttgt cgccggcgag
      121 caggccgccc cccttatcct cgatgccctc gtcatcctcg tcgatcgcga ccccgccggt
      181 caccgtgaaa ccgtcgagca ccgcgtcggc ctgatggcgg tcatcccata tgatctgtat
      241 gacatggtag gcgttctcgt cccgattcag ccactcttcg ttgagatcgt cccatgcgtc
      301 gtcgccgtca agatcgcccg aaaggatggt ctcgttcgcc tcccagtcgc gctcgtcacg
      361 cgccgttccg tcaccgacaa agccgccata gatgccgacg cccggtttca gcgtgaaggt
      421 ctgtttgcgc ggcaggaagg gacaggtctc ctgcggcgag gtgaagcaga aggtcggcgt
      481 gtaggtccct tgcgcgaccc atatctcctg ctggctgttg tcaagcgcct cctctatcgc
      541 atccgacagg tcggtgaagg cgttctccca tgacgatccg ttgttcgcgc cgtcggtcgc
      601 ggcgtggttg acatagatgg tcgcgaggaa aaaacaggcg tcgccggcct cgttgggcag
      661 gtaactcttg ttgcattccc acgcacattc gaaggtctcg ggctcccaaa cggcgccgtt
      721 ctgggtctgt tcgaacttgc cgtcggcgtt ggcgccgatg ccgtgcgcat tttcggggat
      781 gttcaggcag agggtgcgcc gggtcacggg gaccgtgtcc tcgtcggtca ggatatcgtc
      841 ggtcacgaca tcatcaacaa cgacatcgtc ggtgaccaca tcgtcggtga ccacatcgtc
      901 ggtgaccata tcatcggtca ccggctcgtc aatgtcgggc tcgacatcga ccgtgtcggt
      961 atcgggcatg tcgccgtcga gtttatcgcc gtccggtttc gtgtcggtct tcgtgtcgat
     1021 gtccgcatcg tccggctctt cctcgtcagg gatgaggtcg tcggtcagca gatcgccgtc
     1081 gaccgcgatg tcatcggtcg gcaactggtc ggcgtcgctc ttcgtcgtct tcgtgccgtc
     1141 acaggcggcg aggaacagaa aagagagcac aagaataccc atcaaaggat gtctgagaga
     1201 acacatggga aaacctcctc aaaaaagagg gcgcgacagg tcgcgccccc atggatcatg
     1261 caacggtcaa ccgggcgcaa tgaattgcgc ccctacatca tcattgttca tacgcaccca
     1321 tatcgaccac accggctggt cccgtctgta tccgggcatc accaaggatg tcgtacagaa
     1381 tcccttccgg caccagcgcg ttacttccgg cgtcgatgca cggcgatgtc gcctgcaggt
     1441 cgagcgggtc ggcgcccgag ccgacaaaga gcggatcgag atctatgttg ttgccgttgt
     1501 cggtgccaaa c
//
LOCUS       DAOQUE010000604         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_115586_length_1507_cov_3.602617, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000604 DAOQUE010000000
VERSION     DAOQUE010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115586_length_1507_cov_3.602617"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKH10_13455"
     CDS             complement(<1..582)
                     /locus_tag="PKH10_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNT29171.1"
                     /translation="MLEHIRNASPEELAKLAAETRELIIETCSRNGGHLAPSLGTVEL
                     TLALLKVFDLDRDRIVWDVGHQSYAYKILTDRKARFHTLRTMGGISGFPKRSESPYDV
                     FGTGHGGTSISAALGIKEAIKAAGGDGKVIAVIGDGSMTNGLAFEGMNNIESWGRNTL
                     TVLNDNDMFISTSVGGLSHWFSRKLTGPAYAGLR"
     gene            complement(654..1034)
                     /locus_tag="PKH10_13460"
     CDS             complement(654..1034)
                     /locus_tag="PKH10_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29172.1"
                     /translation="MNWEKKAPDRKKRAIVIDDSAPRIETISQGLAEEFSVRTVTSPF
                     KLLSMLCTEKPDLIVLNSSVPWFSTSHFCCAVTNNGLLADTPIVLFNTENTSVNKGNI
                     REFHNCHIIEDTGVISETARRLVS"
     gene            complement(1009..1242)
                     /gene="xseB"
                     /locus_tag="PKH10_13465"
     CDS             complement(1009..1242)
                     /gene="xseB"
                     /locus_tag="PKH10_13465"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNT29173.1"
                     /translation="MEKKGGIAAKFAELDRIAEKLSGGAELEEAMKLYEKGMKLTAEI
                     RDYLAKAEKKIQVIEANGTVTDKEEHELGKEGA"
BASE COUNT          289 a          425 c          454 g          339 t
ORIGIN      
        1 gcgcaacccg gcgtaggcgg gaccggtgag tttgcgggag aaccagtgcg acagcccgcc
       61 gacgctcgtc gagatgaaca tatcgttgtc gttgagcacc gtcagggtgt tacgccccca
      121 cgactcgatg ttattcattc cctcgaaggc gagtccgttc gtcatcgagc cgtcgccgat
      181 gaccgcgatg accttgccgt caccgcccgc cgccttgatc gcttccttga tgccgagcgc
      241 cgccgatatc gaggtgccgc cgtgaccggt gccgaagaca tcgtacggcg actcgctccg
      301 cttcgggaag ccgctgatgc cgcccatggt ccgcagggtg tggaaccgcg ccttgcggtc
      361 ggtcagtatc ttgtacgcgt aggactggtg gccgacatcc cagacgatcc ggtcgcgatc
      421 gagatcgaac accttgagca gggcgagcgt tagttccacc gtgccgagcg acggagcgag
      481 atgcccgccg ttacgggaac aggtctctat gatgagttcg cgggtctcgg ccgcgagttt
      541 cgccaattcc tcgggggagg cgttacggat atgttcgagc atggggactc ctcagaaaca
      601 gaacggcacc catcatagca aggcggcgga agttgtcaat acaaaggaga tggtcaggat
      661 accaggcgcc gcgccgtttc cgatatgacg ccggtgtcct cgatgatgtg gcagttgtgg
      721 aattcgcgga tgttcccctt gttgaccgag gtgttctcgg tgttgaacag cacgatgggg
      781 gtgtcggcga gcaggccgtt gttggtgacc gcgcaacaga aatggctggt ggagaaccac
      841 gggaccgagg aattgagcac gataagatcg ggcttttccg tgcagagcat cgagagcagt
      901 ttgaaagggg atgtcaccgt gcggaccgag aattcctccg cgagcccctg cgatatcgtt
      961 tcgatgcgcg gcgctgagtc gtctatgacg atagcgcgtt ttttgcggtc aggcgccttc
     1021 ttttcccagt tcatgttcct ctttgtcggt cacggtgccg ttggcctcga tgacctgtat
     1081 cttcttttcc gccttggcga gatagtcgcg tatctcggcc gtgagtttca tccctttctc
     1141 gtagagtttc atcgcctcct cgagttcggc gccgcccgac agtttctcgg cgatcctgtc
     1201 gagttccgcg aacttcgcgg cgataccgcc tttcttttcc accggcactt ttctccatgc
     1261 aacaccatat actatggccg cgcgaaagaa aaagtcaagc attacaataa ggtaagtcgc
     1321 attgaaaacc gaatgtatcc ctttaggagt cattcatgta aaaagttgca agcgttcgtc
     1381 ttgtcgctat gatgccgttt gtcgatccca cggaggcgac gatgccgaag aaagagaccg
     1441 aggtcctctg caagataacc tgcaaccggt gcggcaagga gtcgtatgtc cccttcgtcg
     1501 ccgacgg
//
LOCUS       DAOQUE010000605         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_115613_length_1507_cov_2.933196, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000605 DAOQUE010000000
VERSION     DAOQUE010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115613_length_1507_cov_2.933196"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1507)
                     /locus_tag="PKH10_13470"
     CDS             complement(<1..>1507)
                     /locus_tag="PKH10_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNT29174.1"
                     /translation="DHTCVYHDPCEGIDCSGHGECTSGYGYGDEVFCDCDENYHAVGL
                     ECLPGETPDDDQLIPDGDTVTDPCATVTCDEWKECSGGDCILKEGRCEKSDDCGEDEI
                     CDTAAHDCIADPCLAVTCGNGGVCSTSGVTPLCNCPDGFMATGLTCIADTGTKIDWCN
                     VQWPPAIEATVGDAPTRTYSQLYIGGITDGGKPQNAGVSAQLGYGVKTLTYPIIQTDL
                     VWVDADFNITGAGDFGNNHEYMTDFPTAKTGLFNYIYRYSLNEGASWYYCDLNGRITS
                     GAITPGIATISGGGDDCGGVSCAEWESCAIDLCEPLPGRCNMKADCESDEECDLGTHY
                     CVNAAGVLLLDGAVVQGSDAYTFTVRYVGTDELDLAQSVVTLNGVDITATLTYDPTAN
                     TVTISESGIAQKGKYTYLFRAKNSKGAPIEPLYVPLWVETTPFDWRDAFLYQIMNDRF
                     LNGDALNDQPIAGVDAIRNWMGGDWAGIIEKLDGDYFTDLGVNALWISSPVK"
BASE COUNT          253 a          473 c          469 g          312 t
ORIGIN      
        1 tcttgaccgg cgaggatatc cagagcgcgt tcaccccgag gtcggtgaag tagtcgccat
       61 ccagtttctc gatgatgccg gcccaatcac cgcccatcca gttgcggatc gcgtcgaccc
      121 cggcgatcgg ctgatcgttc agcgcgtcac cgttcaggaa ccggtcgttc atgatctggt
      181 agagaaaggc gtcgcgccag tcgaacggcg tcgtctcgac ccagagcggc acatagagcg
      241 gctctatcgg cgcccccttg ctgtttttgg cgcggaatag ataggtgtat ttccccttct
      301 gcgcgatacc gctttccgat atcgtgacgg tgttcgcggt cggatcgtag gtgagcgtcg
      361 cggtgatatc gacgccgttc agcgtcacga cgctctgcgc gagatcgagt tcgtcggtgc
      421 cgacatagcg gacggtgaag gtgtaggcgt cgcttccctg cacgaccgcg ccgtcgagga
      481 ggagcactcc cgccgcattg acgcagtagt gggtgccgag gtcgcactct tcgtcgctct
      541 cgcaatcggc cttcatgtta cagcggcccg gaagcggttc gcagagatcg atggcgcacg
      601 attcccactc ggcgcacgag acgccgccgc agtcatcgcc gccgcccgat atcgtcgcga
      661 tgccgggcgt tatcgcaccg gaggttatcc tgccgttcag gtcgcaatag taccaactcg
      721 ccccctcatt cagagagtag cgatagatat agttgaacag cccggtcttc gcggtcggga
      781 agtcggtcat gtattcgtga ttgttcccga aatcgcccgc gccggtgata ttgaaatcgg
      841 catcgaccca tacgaggtcg gtctggatga tggggtaggt gagcgtcttt acgccgtagc
      901 ccagttgcgc gctcacgccg gcgttctgcg gcttgccgcc gtcggtgatg ccgccgatgt
      961 agagttgact gtaggtacgg gtcggcgcgt cgcccaccgt cgcctcgatg gcggggggcc
     1021 actgcacatt gcaccagtcg atcttcgtcc cggtgtcggc gatgcaggta agcccggtcg
     1081 ccatgaagcc gtcggggcag ttgcacagcg gcgtcacacc gctcgtcgaa cagacgccgc
     1141 cgttaccgca ggtcaccgcc agacaggggt cggcgatgca gtcgtgggca gccgtgtcgc
     1201 atatctcgtc ctcgccgcag tcatcgctct tttcacagcg accctctttc aggatgcagt
     1261 caccgccgga acattccttc cattcatcgc aggtgaccgt tgcgcagggg tcggtcaccg
     1321 tgtcgccgtc ggggatcagt tgatcatcgt caggcgtctc gccgggcaga cattccagcc
     1381 cgaccgcgtg atagttctca tcgcagtcgc agaacacctc gtcaccgtag ccgtagccgc
     1441 ttgtgcactc gccgtggccc gaacagtcga tcccctcgca gggatcgtgg tagacgcagg
     1501 tatggtc
//
LOCUS       DAOQUE010000606         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_116930_length_1497_cov_2.890430, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000606 DAOQUE010000000
VERSION     DAOQUE010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116930_length_1497_cov_2.890430"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PKH10_13475"
     CDS             <1..989
                     /locus_tag="PKH10_13475"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNT29175.1"
                     /translation="NIKALLGLVVTVVLFWLLFRQVDVLLFLRYLAESRWSYVLAGVV
                     LYLSSFIVRGLRWRVLLGHLKKVPLPETIRLVMAGYAVNNVLPGRIGEFTRAYLAGNR
                     NGISRTAVFASIFVERVFDGLTVFLILAALLFTYPFPGWVRVLAETAGLLFVSLFLFI
                     LFSSFSDLPVRFIRAMEERVPRWLALPLTLLEKFMRGTRAIESLGQLAMVLILSFAVW
                     SVELGVYVLIVKAFGVQMPVIAYLLMLVTVNMGMLIPSTPGGLGVFQFAVVKSLEIFA
                     VMTTLGMAVALVLHMAQIVPVTIIGIVWLWRNHVSIASLEKEKRDEGGNGAT"
     gene            complement(982..>1497)
                     /locus_tag="PKH10_13480"
     CDS             complement(982..>1497)
                     /locus_tag="PKH10_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNT29176.1"
                     /translation="DLKASTEKSKEVVRICTQYAQTGMFNIFIAIFSFALAFAFLSSP
                     VSGNEAVSFFVSYLLSIALFGLFQAVFMANAGGCWDNAKKVVEVDLKEKGTTLHEACV
                     VGDTVGDPFKDTSSVAMNPIIKFTTLFGLLAMEIAIAPDFRTIAPYVGIFFLIVAFVF
                     VWRSFYKMRIK"
BASE COUNT          249 a          459 c          465 g          324 t
ORIGIN      
        1 acaatatcaa ggccctgctg gggctcgtcg tcaccgtcgt gctgttctgg cttctgttcc
       61 ggcaggtgga tgtcctgctg ttcctacggt acctcgccga gagccgctgg tcgtatgtgc
      121 ttgccggcgt ggtcctgtat ctttcctcct tcatcgtacg cgggctccgg tggcgcgtcc
      181 tgctcggcca cctgaaaaag gtgccgctcc cggagaccat ccgtctggtg atggccggct
      241 acgcggtcaa caatgtcctg cccgggcgca tcggcgagtt cacgcgcgcc tacctcgcgg
      301 gcaaccgcaa cggcatatcg cgcaccgccg tcttcgcctc gatattcgtc gagcgggtct
      361 ttgacgggct cacggtcttc ctgatactcg cggcgctcct gttcacctat ccattccccg
      421 gatgggtgcg ggtgcttgcc gagacggcgg gtctcctgtt cgtgtcgctg ttcctgttca
      481 ttctgttcag ttccttctcc gacctgccgg tgcggttcat ccgcgcgatg gaggagcggg
      541 tgccccgctg gctcgccctg ccgctcaccc tgctcgagaa gttcatgcgg ggtacgcggg
      601 ccatcgagtc gctcggccaa ctggccatgg tgttgatcct ctcgttcgcc gtctggagcg
      661 tggaactggg cgtctatgtg ctcatcgtca aggcgttcgg cgtgcagatg ccggtcatcg
      721 cgtacctgct catgctcgtg acggtcaaca tggggatgct catcccctcg accccgggcg
      781 ggctcggcgt ctttcagttc gccgtcgtca agtcgctgga gatattcgcc gtcatgacga
      841 cgctcggcat ggcggtcgcg ctcgtgctcc acatggccca gatcgtgccg gtgaccatca
      901 tcggcatcgt atggctctgg cgcaaccatg tgtcgatcgc gtcgctggag aaggaaaaga
      961 gggacgaggg cgggaacggc gctacttgat gcgcatcttg tagaacgaac gccacacgaa
     1021 gacgaaggcg acgatcagga agaatatccc cacatagggc gctatagtgc ggaaatcggg
     1081 cgctatcgct atctccatcg ccagaaggcc gaacagggtc gtgaacttga tgatggggtt
     1141 catggcgacc gaggaggtgt ccttgaacgg atcgccgacc gtgtcgccga ccacgcacgc
     1201 ctcgtgcagc gtcgttccct tttccttcag atcgacctcg accaccttct tggcgttgtc
     1261 ccaacagccg ccggcgttcg ccatgaagac cgcttggaac aggccgaaca gcgctatcga
     1321 gagcagataa ctcacgaaga aggataccgc ctcgttgccg ctgacaggtg aggagaggaa
     1381 cgcgaaggcg agagcgaacg agaatatcgc gatgaagatg ttgaacatgc cggtctgcgc
     1441 gtactgcgtg cagatccgga cgacctcttt cgatttctcg gtggaggcct tgaggtc
//
LOCUS       DAOQUE010000607         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_117261_length_1494_cov_5.031272, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000607 DAOQUE010000000
VERSION     DAOQUE010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117261_length_1494_cov_5.031272"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1494)
                     /locus_tag="PKH10_13485"
     CDS             complement(<1..>1494)
                     /locus_tag="PKH10_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29177.1"
                     /translation="SSSGCEWDDPNGDDDGDGIPNSVEGCGDNDLDGMPNWLDADSDG
                     DGILDSTECPEQPCQNSDKDDTPDFLDKDSDNDGLADKEETTLGTNPYDKDTDGDGSD
                     DLAEIVYNQNNPGGADPKDPDKKIPDGLFYVVLPYNSKEDVQRTLEFSTIIEAIDVAI
                     LVDRSGSMSDEISKVKSEIKPKIIDGIRNTLTISTAFALSYFTWTPNPPYKLLEYVTE
                     DADKVAASVGNIGDANGDDEYLTPALFLLASGVEFHGTVGACLPPAFGGCNQTMIGDA
                     IYDFPKIDCTGQLGNKGGACFRAKSMPIIIIITDEAVKECPKVATSSWDTCQWEIGDP
                     VNIAEAVAAMNGIGAKLIGIDTGFDDNQKETNQLEEGFTLMAEQTGSLDKNGSPFYYH
                     NENPDGSGMTDQIAQAVVSLTTYIDMDVTTGSMSDEQCDGISAAQFVKSSTTVKAEPP
                     DGISNQTATTFESVKQGTIVWFDVRFYNDFCKNTTTNPMTYQALVTVL"
BASE COUNT          247 a          431 c          455 g          361 t
ORIGIN      
        1 agcaccgtca cgagcgcctg ataggtcatc ggattggtcg tcgtgttctt gcagaaatcg
       61 ttgtagaacc ggacatcgaa ccatacgatg gtcccctgct tgaccgactc gaaggtggtg
      121 gcggtctgat tggagatacc gtcgggcggc tcggccttga cggtggtcga ggacttcacg
      181 aactgggccg ccgatatgcc gtcgcactgt tcgtcagaca tactgccggt cgtcacatcc
      241 atgtcgatgt aggtcgtcag cgacacgacc gcctgcgcga tctggtcggt catacccgat
      301 ccgtcggggt tctcattgtg atagtagaag gggctcccgt tcttgtcgag cgacccggtc
      361 tgctcggcca tgagcgtgaa gccctcctcc agttggttcg tttccttctg gttgtcgtcg
      421 aatccggtgt ctatgccgat gagtttcgcg ccgatgccat tcatcgcggc gaccgcctcg
      481 gcgatgttga ccggatcgcc gatctcccat tggcaggtgt cccatgagga ggtcgctacc
      541 ttggggcact ccttgaccgc ttcgtcggtg atgatgatga tgatgggcat cgatttggcg
      601 cggaaacagg caccaccctt gttccccaac tgaccggtgc agtctatctt cgggaaatcg
      661 tagatcgcgt cacctatcat ggtctggttg caaccgccga acgcgggggg caggcaggcg
      721 ccgaccgtcc cgtggaactc gacaccgctc gcgagcagga acagtgccgg ggtcaggtat
      781 tcgtcgtcgc cgttcgcgtc gccgatgttg cccaccgagg cggcgacctt gtcggcatct
      841 tcggtaacat actcgagcag tttatagggc gggttggggg tccaggtaaa gtaggagagg
      901 gcaaaggcgg tgctgatggt cagcgtgttg cgtatcccgt cgattatctt tggttttatc
      961 tccgatttga ccttgcttat ctcgtcgctc atcgaaccgg aacgatcaac aagtatcgcg
     1021 acatctatcg cttcgatgat ggtcgagaat tcaagcgtcc gttgcacatc ctccttcgag
     1081 ttatagggca ggaccacata gaagagcccg tcgggtatct tcttgtcggg atccttcggg
     1141 tcggcgccgc ccggattgtt ctggttgtac actatctcgg cgagatcgtc ggagccgtcg
     1201 ccgtcggtgt ccttgtcgta cgggttggtc ccaagcgtcg tctcctcctt gtcggcgaga
     1261 ccgtcgttgt cggaatcctt gtcgaggaaa tcgggcgtgt cgtccttgtc gctgttctgg
     1321 cacggctgtt caggacactc ggtgctgtcg aggataccgt caccgtccga atcggcatcg
     1381 agccagttgg gcataccatc cagatcgttg tcgccgcacc cctccacgct gttgggtata
     1441 ccgtcgccgt cgtcgtcgcc gttcgggtcg tcccactcgc agccgctcga cgaa
//
LOCUS       DAOQUE010000608         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_117265_length_1494_cov_4.654621, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000608 DAOQUE010000000
VERSION     DAOQUE010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117265_length_1494_cov_4.654621"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="PKH10_13490"
     CDS             <1..1255
                     /locus_tag="PKH10_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protein kinase PrkA"
                     /protein_id="HNT29178.1"
                     /translation="RRGALARVAGGGIHFADEIFKNKRDLVQVYLGVIQNRTIDIEGF
                     KWPLDSLIVATSNNAEFARFLEEKEQAPIVDRCRLCYMSHNTNLKLQKELTAFAIGGL
                     EKTTFTGQSLHVDPNLNHAISASVVLSRMPRSDKLTPVEMMKLAAGEVAGEKSIKTLA
                     EVIGELNTDPDITKRFGQKGLGHRNLGRAVQVLLERSETQEGKCMYAGDAFKALESVI
                     LDYVQDANDRVKYFNDLKLAKGLYREQVMTSIFNAYMDEPNAIERDVMNYVNMIIGID
                     AKNLGPDKLWTYRDPQTGKLVSMKIDETFINSVEERMGLKNSEQKHSYRTTISKIYGQ
                     KVIKDPNYNFMDNNDLVKAVTDVRLQSDIAGAGSLVGALANRTNEDNQKLYGRMIATM
                     TGKLGYCATCAQKTIEYFCTQDDAG"
     gene            1262..>1494
                     /locus_tag="PKH10_13495"
     CDS             1262..>1494
                     /locus_tag="PKH10_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29179.1"
                     /translation="MTDRPIPAVDDDGAALDAERRLRKRRIVRLSLAVDAAIILAVVL
                     FIYIAGSSFHETVRDHDMVAIIVLKDPQDPAKQ"
BASE COUNT          355 a          483 c          425 g          231 t
ORIGIN      
        1 gcggcgcggc gcgctggccc gcgtggcggg cggcggcatc cacttcgccg acgagatatt
       61 caagaacaag cgcgacctcg tgcaggtcta tctcggcgtc atccagaacc gcacgataga
      121 catcgaaggg ttcaagtggc cgctcgactc gctcatcgtc gcgacctcga acaacgccga
      181 gttcgctcgc ttcctcgaag agaaggagca ggcccccatc gtcgaccggt gccgcctgtg
      241 ctacatgtcg cacaacacca atctcaaact gcagaaggaa ctcaccgcgt tcgccatcgg
      301 cggactcgag aagaccacct tcaccggcca gagcctgcat gtcgacccga acctcaacca
      361 cgcgatatcg gcgtcggtcg tcctgtcgcg gatgccgaga tcggataaac tcaccccggt
      421 cgagatgatg aaactcgcgg cgggcgaggt ggcgggcgag aaatccatca agacgctcgc
      481 cgaggtgatc ggcgaactca acaccgaccc cgacatcacc aagcggttcg gccagaaagg
      541 gctcggccac cgaaaccttg gccgcgcggt gcaggtgctc ctcgagcgca gcgaaacaca
      601 ggagggtaag tgcatgtacg ccggcgacgc cttcaaggcg ctcgaaagcg tgatactcga
      661 ctatgtgcag gacgccaacg accgcgtgaa gtacttcaac gacctgaaac tcgccaaggg
      721 tctgtaccgc gaacaggtga tgacctcgat attcaacgcc tacatggacg aaccgaacgc
      781 gatagagcgc gatgtcatga actatgtcaa catgatcatc ggcatcgacg cgaagaacct
      841 cggccccgac aaactctgga cctaccgcga tccgcagacg gggaaactgg tgtcgatgaa
      901 gatagacgag accttcatca acagcgtcga ggagcggatg gggctcaaga acagcgagca
      961 gaaacactcc taccgcacga ccatctcgaa gatatacggc cagaaggtca tcaaagatcc
     1021 gaactacaac ttcatggaca acaacgacct cgtgaaggcg gtgaccgatg tgcgcctgca
     1081 gtccgacatc gccggcgccg gttcgctcgt cggtgcgctc gccaatcgga ccaacgagga
     1141 caatcagaaa ctctacggtc gcatgatagc cacgatgacc ggcaaactcg gctactgcgc
     1201 cacctgcgcc cagaagacca tcgagtactt ctgcacgcag gacgacgccg ggtagccgtc
     1261 catgaccgac cgaccgatcc ccgccgtgga cgacgacggc gcggccctcg acgccgaacg
     1321 gcgcctcaga aagcgtcgta tcgtgcggct ctcgctcgcg gtggatgccg cgatcatcct
     1381 tgccgtcgtg cttttcatct acatcgccgg ctcgtcgttc cacgaaacgg tacgggatca
     1441 cgatatggtc gccatcatcg tgctcaaaga cccgcaggac cccgcaaaac agtg
//
LOCUS       DAOQUE010000609         1488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_118062_length_1488_cov_4.474529, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000609 DAOQUE010000000
VERSION     DAOQUE010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118062_length_1488_cov_4.474529"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PKH10_13500"
     CDS             <1..1049
                     /locus_tag="PKH10_13500"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNT29180.1"
                     /translation="MDASGKIIIKPQYLKVRNFSSGLAAVLVLVKEKKDSKMLWGYID
                     TKGKMVIKPAYEAADDFGGDLAGVQINKRWGFIDKTGKVVIDFLYDATGDKFEEGIIM
                     VKVGEKYGLINTKGEVVLEPTYKNFRPFRDGFAAANDYSIGCKLYVGRNGKVLPVDID
                     KIDSVSDFKDGFAVISRYNNNAVIGTDGTMVFKNSHSINWVSEGVVCASHEVEGNIKI
                     SLYNIRTKKWIGDSFEDHYKFSEGLAGACTNKRCGFIDKEGAIVVPLTYDEVRPFNDG
                     LAPVRIGRTWGYIDKTGKKIIDLQFDNAGPFANGVGRAVKDSEDVYINKTGKVIGAGG
                     LADGESKWIDYDEW"
     gene            1115..>1488
                     /locus_tag="PKH10_13505"
     CDS             1115..>1488
                     /locus_tag="PKH10_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HNT29181.1"
                     /translation="MKDITKINKTIFEKIKQINEYSQEFWSVRHLAKALDYDDLRNFL
                     NVVEKAKESCKNSGFDIADHFVDITEMIETGKGATREFPSIALSRYACYLIVQNADPS
                     KEIVALGQTYFAYQTRKQEIQE"
BASE COUNT          448 a          333 c          379 g          328 t
ORIGIN      
        1 tcatggatgc aagcgggaag atcatcatca agccgcagta tctcaaagtg cgaaacttct
       61 cttccggcct cgcggcggtc cttgttctcg taaaagaaaa gaaggactcg aaaatgcttt
      121 ggggatacat agacacaaaa ggaaaaatgg ttatcaaacc cgcatacgaa gccgccgatg
      181 atttcggcgg cgaccttgcg ggagtacaaa tcaataagcg ttggggattc atcgacaaga
      241 cgggaaaagt ggtgatcgat tttctctacg atgccaccgg cgacaaattc gaggagggca
      301 taataatggt caaggtcggc gagaaatacg gcctgatcaa tacgaaggga gaggttgtat
      361 tggagcccac atacaaaaat ttcagacctt ttcgcgatgg ctttgccgcc gcgaacgatt
      421 actcaatcgg gtgtaaattg tatgttggca ggaacggcaa agttctgccg gtcgatatcg
      481 acaagatcga ttctgtttct gactttaaag acggttttgc ggtgatcagc agatacaaca
      541 ataatgcggt gatcggcaca gacggaacca tggtatttaa gaactcccac agtattaatt
      601 gggtcagcga gggtgtcgtc tgcgcttcgc atgaagttga aggaaacatc aaaatctctc
      661 tctacaacat cagaaccaag aaatggatcg gtgattcatt cgaagatcac tataaattct
      721 ccgaagggtt ggcaggtgcc tgtacgaata agcggtgcgg cttcatcgac aaggagggag
      781 cgatagtcgt ccccttgaca tacgacgagg tgcgtccctt taacgacggc ttggcaccgg
      841 tgagaatcgg ccgtacatgg ggctatatcg acaaaaccgg aaagaaaatc atagatcttc
      901 aattcgataa tgccggtcct tttgccaacg gcgtcggccg tgcggtgaaa gattcagaag
      961 atgtatatat aaataaaact ggcaaagtga tcggcgctgg cggccttgcc gatggtgaat
     1021 cgaagtggat tgactacgac gaatggtagc ggaaaactaa tgtccgaccg tgaacgcgcc
     1081 ataaatcatt ttttccgata gggggcggct gaccatgaaa gacatcacga aaatcaacaa
     1141 aacgatcttt gaaaagatca aacagatcaa cgagtatagt caagaattct ggtctgttag
     1201 acaccttgct aaagcgctcg attacgacga cctgagaaac ttcttaaatg tcgtcgaaaa
     1261 ggcaaaagaa tcatgtaaaa acagcgggtt cgatatcgcc gatcatttcg ttgatatcac
     1321 cgagatgata gagaccggta aaggagccac gcgcgaattt ccgtcaatag ccctttcccg
     1381 ctatgcctgt tacctcatcg tccagaacgc cgacccgtcc aaagagatcg tcgcgctcgg
     1441 acaaacctac ttcgcctacc agaccagaaa gcaggagata caggaaca
//
LOCUS       DAOQUE010000610         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_118999_length_1481_cov_4.147966, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000610 DAOQUE010000000
VERSION     DAOQUE010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118999_length_1481_cov_4.147966"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /gene="eno"
                     /locus_tag="PKH10_13510"
     CDS             <1..938
                     /gene="eno"
                     /locus_tag="PKH10_13510"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNT29182.1"
                     /translation="ISMAAAPAAAHALRLPLFRYLGGVTSDTLPVPLSNILNGGAHAD
                     NTVDCQEFMIAPLGASSFREAIRWNAEVFHTLKKILKDKGLATGVGDEGGFAPNLRSD
                     EEALAVIVSAIEKAGFKPGSDIMLALDVAASGMYENGVYTFLKSTGEKRTSVQMVDLY
                     EKLVNNYPIFSIEDGMAEDDWEGWKLMTDRLGEKIQIVGDDLFVTNIERLQRGISEGS
                     ANSILIKLNQIGTVTETLDTIAHAHRSGFTTVISHRSGETEDTFIADLAVAVNAGQIK
                     TGSLCRSERIAKYNQLLRIEEFLGDMAFYPGDSIR"
     gene            960..>1481
                     /locus_tag="PKH10_13515"
     CDS             960..>1481
                     /locus_tag="PKH10_13515"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNT29183.1"
                     /translation="MTTAQKRPVDKGFTVVRAVALGAFVIFAGFFLHYIAKPEGSRNM
                     YLLEMLLYDAQVKMLPDGEASDKFVIAAADDKALAAYGRWPWDRGVIADLVETMMERG
                     AKGVVFDMTFSERVNRDLEAKIAALDPAWVPGDAKLKATLDKYGRKVVLGAILQTPAE
                     ATDAAAAQAAIQVA"
BASE COUNT          306 a          456 c          471 g          248 t
ORIGIN      
        1 gcatctcgat ggccgccgca cccgcagcag cccacgctct gcgcctgccg ctcttccggt
       61 atcttggcgg cgtgacgagc gacaccctgc cggtcccgct gtcgaacata cttaacggcg
      121 gcgctcacgc cgacaatacc gtcgactgtc aggagttcat gatagcgccg ctcggcgcct
      181 cgtcgttccg cgaggcgatc cgctggaacg ccgaggtgtt ccacacgctc aagaagatac
      241 tcaaggacaa aggtctcgcc accggcgtcg gcgacgaagg gggcttcgcg ccgaacctca
      301 ggtcggacga ggaggcgctc gcggtcatcg tctcggcgat agagaaggcg ggcttcaaac
      361 cggggagcga tatcatgctt gcgctcgatg tggcggcgag cggcatgtac gagaacggcg
      421 tctacacgtt cctcaaatcg acgggcgaga agagaacgag cgtccagatg gtcgacctct
      481 acgagaaact ggtcaacaac tatcccatct tctccatcga ggacgggatg gctgaggatg
      541 actgggaagg ctggaaactg atgaccgacc ggctcggcga gaagatacag atagtcggcg
      601 acgacctgtt cgtgacgaac atcgaacgac tccagcgcgg tatcagcgag gggagcgcca
      661 actccatact catcaaactc aaccagatcg gcacggtcac cgaaacgctc gacaccatcg
      721 ctcatgcgca ccgtagcggc ttcaccaccg ttatatcgca ccgttcggga gagaccgagg
      781 acaccttcat cgccgacctt gccgtcgcgg tcaatgcggg ccagataaag accggatcgc
      841 tctgtcgcag cgaacgcatc gctaagtaca atcaactcct gcgcatcgag gaattcctcg
      901 gcgacatggc cttctatccg ggtgacagca tccgttagtg ttctccggga ggggatgaca
      961 tgaccaccgc acagaaaagg ccggtcgaca aaggtttcac cgtcgtccgt gccgtcgcgc
     1021 tcggcgcctt cgtcatcttt gcgggttttt tcctgcacta catcgcgaag cccgagggaa
     1081 gccgcaacat gtacctgctc gagatgctcc tctacgacgc gcaggtcaag atgctgcccg
     1141 acggggaggc atcggacaag ttcgtcatcg cggcggccga cgacaaggcg ctcgcggcgt
     1201 acggtcgctg gccgtgggac cgtggcgtca tcgccgatct cgtcgagacg atgatggagc
     1261 ggggcgcgaa gggggtcgta ttcgacatga ccttctcgga gcgcgtgaac cgcgatcttg
     1321 aggcgaagat agcggcgctt gacccggcgt gggtgccggg cgacgcgaaa ctcaaggcga
     1381 cgctcgataa gtacggccgt aaggtggtgc tcggagccat cctgcagacg cccgccgagg
     1441 cgaccgacgc ggcggcggcg caggcggcga tacaggtggc g
//
LOCUS       DAOQUE010000611         1479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_119293_length_1479_cov_3.019663, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000611 DAOQUE010000000
VERSION     DAOQUE010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119293_length_1479_cov_3.019663"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1479
                     /locus_tag="PKH10_13520"
     CDS             <1..>1479
                     /locus_tag="PKH10_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNT29184.1"
                     /translation="RLSGEWDGAERVVREAVPLFMLDVEEGRLYLDLYLTVKGDRVKI
                     PPRRRESEQVYARDGRLLRFDPAAVKDARRIVRRAGFRMSGDRYTAPVSTLAGLLADD
                     SPLRAAGTVGTARPVRRIEIPEGMMDHSEIGIEAHPGEGWFSFSLRFPEAEVPLPPES
                     LVDAMRQVLKGNGDPVVADRNGDPVVIEKSRAFLLRLRDLLLFGQAMPGERVSAALLP
                     GILRQKGRMVLDRFSGDDADRAAYEGIIRAFSSGTLPEVPDLPGFEVLRPYQREGVRW
                     LNLIGRMGLGAILADEMGLGKTLQTLAALQVDGGKGVTLVIAPKTLVWSWEREVQKFF
                     PSLDRTVIDAAAPQDRARRWRAVSSGVVITSYAIAVNDIELLKQKRFRAVVVDEAQHL
                     KNQKTKRFGALQKLRAGLRIALTGTPIENHLADLWSIFQFLIPGLLGRRQDIERMERE
                     GDAAGFARIAALTAPFVLRREKRQILPELPPVIIKEYPVDMT"
BASE COUNT          245 a          457 c          518 g          259 t
ORIGIN      
        1 tccgcctgag cggcgaatgg gacggcgccg aacgggtggt gcgcgaggcg gtgccgcttt
       61 tcatgctgga cgtggaggag ggacggctct atctcgacct ctacctcacc gtgaaggggg
      121 accgcgtgaa gataccgccg aggcggcggg agagcgaaca ggtctatgcg cgggacggcc
      181 gtctgctccg gttcgatccc gccgcggtga aagatgcgcg ccgtatcgtc cgccgcgcgg
      241 gattccggat gagcggggac cgctacacgg cgccggtctc gaccctcgcg gggcttcttg
      301 ccgatgattc gccgcttcgc gccgcaggga cggtgggaac ggcgcgtccg gtacgacgca
      361 tcgagatacc ggaaggcatg atggatcatt ccgagatcgg tatcgaggcg caccccggcg
      421 aagggtggtt ctccttttcg ctccgtttcc ccgaggccga ggtgccgctg ccgccggaaa
      481 gcctcgtcga tgcgatgcgg caggtgctca agggtaacgg ggatccggtg gtggccgacc
      541 gcaacggcga tccggtcgtc atcgaaaaaa gccgggcttt cctgttacga ctccgcgatc
      601 ttctgctttt cgggcaggcg atgcccggcg aacgggtaag cgccgccctt ctccccggca
      661 tactccgcca gaaggggcgg atggtgttgg accgcttttc cggtgacgat gccgaccgcg
      721 ccgcgtacga ggggatcatc cgcgcctttt cctccgggac gcttcccgag gtgcccgacc
      781 tgccggggtt cgaggtgctg cgtccctatc agcgcgaggg agtgcgctgg ctgaacctga
      841 tcggtcggat ggggctcggc gccatactcg ccgacgagat gggactcggc aagacgctcc
      901 agacgctcgc ggccctgcag gtggacggcg ggaaaggggt gacgctggtc atcgcgccca
      961 agacgctcgt ctggtcgtgg gaacgcgagg tgcagaaatt cttcccgtcg ctcgaccgca
     1021 ccgtcatcga tgcggcggct ccgcaggacc gggcgcggcg ctggcgggcc gtgtcgagcg
     1081 gcgtggtcat cacttcctac gcgatagcgg tgaacgatat cgagttgctg aaacagaaac
     1141 ggttccgcgc cgtcgtcgtg gacgaagcgc agcacctgaa gaatcagaag acaaagagat
     1201 tcggcgcgtt acagaagttg cgggccggtc ttcgcatcgc gctgaccggc accccgatcg
     1261 agaatcatct cgccgatctg tggagcatct tccagttcct tatccccggt cttttgggcc
     1321 gtcgacagga tatcgagcgg atggagcgcg agggggacgc cgcggggttc gcccggatcg
     1381 ccgcactgac ggcgcccttc gtcctgcgcc gggaaaaacg gcagatactt cccgagttgc
     1441 ccccggtcat catcaaggag tatccggtcg acatgaccg
//
LOCUS       DAOQUE010000612         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_119544_length_1477_cov_3.265120, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000612 DAOQUE010000000
VERSION     DAOQUE010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119544_length_1477_cov_3.265120"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..>1477)
                     /locus_tag="PKH10_13525"
     CDS             complement(103..>1477)
                     /locus_tag="PKH10_13525"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNT29185.1"
                     /translation="GAETVTADIHRNGDKYCFAVRATDAVYAQDGTILATNRAPVLST
                     NVVDQGDVQLTTHELIAASAGNHTINIKNMGDINGDGRTDFAVSDSGQQKAYVFTSNA
                     TGAPTKLELTKPADAGTSFGYRLAVADLDGNEHKDVVVSDIGGKNLHIYYGTDGSVNV
                     ERGDKVTLLDAIYDIAGVDDYNGDGCDDLAVGQAFYDDPGDATTDDRTNRVLVLYGDD
                     KGASGKTCIGDYAAQVGVSHIGKAAGERFGYNVKKVGDIKKGETAYGCFVGGHYDNVN
                     PANAKITNGKVFYGSSSPTEARVTTYDFTVSGLSQIRTMFSGELNGDDHADFAVISGG
                     GEIRFFFGSEAGTVFPGTWTEGVNRVTRSVIMDELVGVETGAWGVGNSGASEDVDGDG
                     LADTFISGVQSQSLYSGRDTFFTAHPSVYLSLPVTSGNVQTTVLSYGLVICDRNADTG
                     NCVLYYH"
BASE COUNT          321 a          429 c          396 g          331 t
ORIGIN      
        1 cacatctcgt cattgagata acaggtgaaa agaaggcgag tttcgatgat cttttcttcg
       61 ataacctgtg gtttgatgta cctcactcta tcctccccga tctcaatgat agtaaagaac
      121 acaattgccg gtgtcagcgt tacgatcaca gatcaccagt ccgtaggaga gcacggtcgt
      181 ctgcacattc cccgatgtga cgggaagaga caggtacacc gaaggatgcg cggtaaagaa
      241 agtgtcccga ccgctgtaaa gtgactgtga ctgtactccg ctgatgaaag tatcggcaag
      301 cccgtcgcca tcgacgtctt cggaagcacc ggagttaccg acaccccacg caccggtctc
      361 aactcccacc agttcgtcca tgatgacact tctggtcacc cggttgacgc cttcggtcca
      421 agttcccggg aataccgttc ccgcttcgga cccgaagaag aagcgtatct cgccgcctcc
      481 agagataaca gcgaaatcag cgtgatcgtc cccgttcagt tcaccggaaa acatcgtgcg
      541 gatctgcgag agtcccgaaa cggtgaaatc gtaggtggtc acgcgggcct ccgtcggaga
      601 ggaacttccg tagaatacct tgccgttggt gatcttggcg ttcgccgggt tcacattgtc
      661 ataatgccca ccgacgaaac acccataagc cgtttcgcct tttttgatgt cgccgacctt
      721 tttgacgttg tacccgaaac gttcgccggc ggctttgcct atatgggaaa cacctacctg
      781 tgccgcataa tcaccgatac aggtcttgcc ggaagcgccc ttgtcatcac cgtacaggac
      841 aagcaccctg ttggtccgat catcggtcgt cgcatctccg ggatcgtcgt agaaagcctg
      901 cccgacggcg agatcatcac aaccatcgcc attataatcg tcgaccccag cgatatcgta
      961 gatggcatca aggagggtca ccttgtcccc gcgctcgaca ttgacggaac cgtcggtgcc
     1021 ataataaatg tgaagatttt tgccaccgat gtcgctgacc acgacatcct tgtgttcgtt
     1081 gccgtcgaga tcagccaccg ctaggcgata accgaaagag gtcccagcgt cggcgggttt
     1141 ggtcagttcg agtttggtcg gtgcacccgt agcattgctg gtgaacacat aggccttctg
     1201 ctggcccgaa tcggaaacgg cgaaatcggt acgaccgtcg ccgttgatgt cgcccatatt
     1261 cttgatgttg atggtatgat tgcccgccga ggccgctatc aattcgtgcg tcgtcaactg
     1321 cacatctccc tggtccacga cattagtaga caggaccgga gcgcggttgg tggcaagaat
     1381 cgtgccgtcc tgcgcgtaga ccgcgtcggt cgcccggacc gcgaagcagt acttgtcgcc
     1441 gttccggtgg atgtcggcgg tgaccgtttc ggcgccg
//
LOCUS       DAOQUE010000613         1453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_122833_length_1453_cov_3.283262, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000613 DAOQUE010000000
VERSION     DAOQUE010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122833_length_1453_cov_3.283262"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PKH10_13530"
     CDS             complement(<1..890)
                     /locus_tag="PKH10_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29186.1"
                     /translation="MGILRTSCILLGAVILLFGCESGTKKFVPKDDVALGDSDELLAD
                     SDDIWTDEEWDWETKDGDMMGEDDPFTGDLDIVPDSDQKPDTCGNGGVDLGEVCDGGL
                     KNCIEIDPMKYKAGKAACLDTCLGWDTVTCEEYEYECGDGDVDFPEVCDTEELTDCVD
                     IDPMKYKAGKAYCLDTCLGYDTATCEEIDEDDDIVPDTTDVQPESEGMVDQDLIQPDT
                     ADVQPDPDTVDADHVEADILPDNDTVCTHTCDTLNEAYCNGTVVMQCQIGIDGCRHWA
                     EEIDCFDTGRLCNDNNEVGD"
     gene            complement(1025..>1453)
                     /locus_tag="PKH10_13535"
     CDS             complement(1025..>1453)
                     /locus_tag="PKH10_13535"
                     /inference="COORDINATES: protein motif:HMM:NF025099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HNT29187.1"
                     /translation="TIVRLGRFLTVEMGGGAFPILKRNRHLATLVPIVPAFLLGVSGG
                     WKTVWPVFGAANQLVGALVFIILTSYLYSRGKPIRYTLWATLFMLLTTIGALLLLAWG
                     YFLGSPPQYVLGSISVALILLALLMAMEGVKLFSRVRSRA"
BASE COUNT          313 a          459 c          379 g          302 t
ORIGIN      
        1 tcatcgccca cctcattgtt atcgttgcac aacctgcctg tatcgaaaca gtcgatctct
       61 tcggcccaat gacgacagcc atcgatgccg atctggcact gcatgaccac cgtgccgtta
      121 cagtaagcct cgttcagcgt atcacaggta tgggtgcaga cggtgtcgtt atcgggaagg
      181 atatcggcct cgacatggtc cgcatcgacc gtgtcggggt cgggctggac atcggccgtg
      241 tcgggctgga tgaggtcctg gtcgaccatc ccctcggact cgggctggac atcggtcgtg
      301 tcgggtacga tgtcgtcatc ctcgtcgatc tcctcgcagg tggcggtgtc gtagccgaga
      361 caggtgtcca gacagtaggc cttacccgcc ttgtacttca tcggatctat gtcgacgcaa
      421 tcggtgagct cctcggtgtc acacacctcg ggaaaatcaa catcgccgtc accgcattcg
      481 tattcgtact cctcgcaggt caccgtgtcc cagccaagac aggtgtccag acaggccgcc
      541 ttgcccgcct tgtacttcat tggatctatc tcgatacagt tcttcaggcc cccatcgcac
      601 acctcgccca ggtccacacc accgttcccg caggtgtcgg gcttctgatc gctatcgggc
      661 acgatatcga ggtctcccgt gaaaggatcg tcttctccca tcatgtcgcc atctttcgtt
      721 tcccagtccc actcctcatc ggtccagata tcgtcactgt cggcgagcag ttcgtcactg
      781 tcacccagcg ccacatcgtc cttcggcacg aatttcttcg tcccgctctc gcaaccgaac
      841 agcaagatga cggcacccag tagaatgcat gatgttctca ggatacccat cgttttcccc
      901 ctcttaaaaa atgagcttaa aagccggaca gcggcacact ataaccggtt gtcctctttt
      961 tgacaagtat ttcgccctcc gtgtcgcaga caaaagaagt gcgatatcgc tcacatggtc
     1021 atgctcaagc gcgggaacgg acgcgggaaa aaagtttcac cccctccatc gccatcagaa
     1081 gggcaagaag gatcaaggca acggagatgc tccccaacac atactgggga ggcgatccaa
     1141 ggaaatagcc ccacgcgagt aagagaagtg cgccgatcgt cgtgagcaac ataaagaggg
     1201 tcgcccagag cgtgtagcgt atcggcttgc ctcgactgta gaggtaactg gtcaggatga
     1261 tgaaaactag cgcgccgacc agttggttgg ccgccccgaa gaccggccag accgtcttcc
     1321 agccgccgct cacgcccaaa aggaaggccg gaacgatcgg caccagcgtc gccagatgcc
     1381 ggttacgctt gaggatcggg aaggccccgc cccccatctc gaccgtcagg aaacgcccca
     1441 acctcacgat agt
//
LOCUS       DAOQUE010000614         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_122940_length_1452_cov_4.773085, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000614 DAOQUE010000000
VERSION     DAOQUE010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122940_length_1452_cov_4.773085"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PKH10_13540"
     CDS             <1..1019
                     /locus_tag="PKH10_13540"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT29188.1"
                     /translation="AGVRMVRMHSLALRSMRLPAKFLNWTSFYVIVFFYGLLLRRHDI
                     VMALTTPPYIGLVGWFIAAVKRAKFVLNVQDLYPEVLGAAKVKASFLAPLFIPFSKLL
                     YRLSDKIVVIGDVMADRILSKNPGLHESRITTIENWANGAEITMVDPAENRYKGKWGV
                     TDEFVILYSGNMGRAHNFTVLHELMRRYREEPKVLFLFIGGGPKERNIRFFCRTNDIR
                     NVQFHGYVPRADLAHTYSLGDLAVITMEEGTEGLVVPSKFYAYAAAGLPVLCLSAQET
                     DLRRHIREPWGRITRDADDAFRFIEEIRQSEYKHHDDIRRYFEEHLDRPLRTARYRDL
                     FAGL"
     gene            complement(1035..>1452)
                     /locus_tag="PKH10_13545"
     CDS             complement(1035..>1452)
                     /locus_tag="PKH10_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29189.1"
                     /translation="LLSNLMLFCDDAALIERTVAMMTENNPHNDAKRIETKKIFVVLL
                     ARLGERDQRHIRTLRTLLNHPSVEHGARQEILRVLRDLPGRFETMTATDTPPPPPPPD
                     DPEREENTLVSPLFGGAHDPLDDDSEETTVIEKRPG"
BASE COUNT          243 a          445 c          475 g          289 t
ORIGIN      
        1 gcgccggggt acgcatggtc cggatgcact cgctcgccct gcgcagcatg cgattgcccg
       61 cgaagttcct caactggacc tcgttctatg tgattgtctt cttctacggg ctccttctgc
      121 ggcgtcacga catcgtgatg gcgctcacca ccccgcccta catcgggctg gtcggctggt
      181 tcatcgcggc ggtgaagcgg gcgaagttcg tcctcaatgt gcaggacctc taccccgagg
      241 tgctcggcgc ggcgaaggtc aaggcgtcgt tcctcgcgcc gctcttcatc ccgttctcga
      301 aactgctcta ccgcctgtcg gacaagatcg tggtcatcgg cgatgtgatg gccgaccgga
      361 tactatcgaa gaacccgggc ctgcacgaga gccgcatcac gacgatagag aactgggcga
      421 acggcgccga gataacgatg gtggacccgg cggagaaccg ttacaagggg aagtggggcg
      481 tcaccgacga attcgtgata ctctattcgg gcaacatggg gcgggcacac aatttcacgg
      541 tgctccacga actcatgcgg cggtaccgcg aggagccgaa ggtgctgttc cttttcatcg
      601 gcggcgggcc gaaagagcgc aacatccggt tcttctgccg caccaacgac atcaggaatg
      661 tgcagttcca cggctatgta ccgcgcgccg atctcgccca cacctattcg ttgggcgatc
      721 tcgccgtcat cacgatggag gaggggaccg aagggctcgt ggtgccgtcg aaattctacg
      781 cctacgcggc ggccggcctg ccggtgctgt gcctttcggc gcaggagacc gatctgcgcc
      841 gccatatccg cgagccgtgg ggccgcatca cgcgcgacgc cgacgacgcg ttccggttca
      901 tcgaggagat acggcagagc gagtacaagc accacgacga catccgtcgc tatttcgagg
      961 agcacctcga ccgtccgctc aggacggcgc ggtaccgcga cctattcgcg gggttgtgag
     1021 actgttctcc tgaactatcc cggtcgtttc tctatgacgg tcgtttcttc gctgtcatcg
     1081 tcgagcgggt cgtgcgctcc gccgaaaagc gggctgacga gtgtgttctc ttcgcgttcg
     1141 gggtcgtcgg gcggcggcgg cggcggcggc gtgtcggtcg cggtcatggt ctcgaaccgg
     1201 cccggcaggt cgcgcaggac ccgcaggatc tcctgccgcg cgccgtgttc gaccgacgga
     1261 tggttgagga gcgtccgcag ggtacggata tgtcgctggt cccgttcgcc gagccgggcg
     1321 aggagcacga cgaatatctt tttggtctct atccgtttgg cgtcgttgtg cgggttgttc
     1381 tcggtcatca tcgccaccgt gcgctcgata agcgccgcat catcgcagaa gagcatgagg
     1441 ttggagagga gc
//
LOCUS       DAOQUE010000615         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_123964_length_1445_cov_3.364029, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000615 DAOQUE010000000
VERSION     DAOQUE010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123964_length_1445_cov_3.364029"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..>1445)
                     /locus_tag="PKH10_13550"
     CDS             complement(172..>1445)
                     /locus_tag="PKH10_13550"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT29190.1"
                     /translation="IAKDPAMERRFTLVPIEEPEGEALLAIAQESARSLSFFHHVGLD
                     DPAFVKQAIALSTRYMAGRSQPDKVLSLLDTLGSVLQRSGRKEATDRDLLEFISAKSR
                     IPMENLLVDGTRILQTLPERLDDAIKGQAEAKRRIVQLLARRFVRRDTRRPIASLLLA
                     GPSGVGKTETARRLAEYLFGSADRMVIFDMSEFQEQHAVSRLIGSPPGYTGYEDGGRL
                     TEAFRREPYQMLLLDEIEKAHPKVLGILLQILDEGRVTDSRGFTVAMSQAIVVMTTNL
                     GAEELATPRLGFGNGGDRAQQTDAPVRAKIEKALSPEMANRIDEIVVFSPFSAEELPA
                     VTRHLLERTVHGLRENYGCDITVDDPDAFAAAVVAALAPADRSGGARSIRRWLERRLE
                     SLVLERIKQQTSAEPIRVSFEMLNDPSAPKE"
BASE COUNT          244 a          459 c          448 g          294 t
ORIGIN      
        1 agggttggtt ccttattttc tctctctttg ctgctgctca gttttcaatg aactcaggtc
       61 cggtacgcga ccggccagcg ggcggcacta aaacacagat cgacccgctt gtcaatcttt
      121 tttcattctt ttttatacaa gagcgaaaaa gtgggcgatc ccgtcgcttt tttattcttt
      181 cggtgcggac gggtcgttca gcatctcgaa ggagacccgt atcggttccg cggaggtctg
      241 ctgttttata cgctccagca cgagcgactc caaccggcgt tccagccagc gcctgatgct
      301 gcgggcgccg cccgagcggt cggcaggagc caacgccgcg accaccgcag cggcgaaggc
      361 atcgggatca tccaccgtga tgtcgcatcc gtaattctcc cgcaggccgt ggaccgtccg
      421 ttccagcagg tgccgcgtca ccgccggcag ttcctctgcg ctgaacggcg agaacacgac
      481 tatctcgtcg atgcggttgg ccatctccgg agagagcgcc ttttctatct tggcacggac
      541 cggtgcatcg gtctgttgtg cacgatcgcc tccgttaccg aacccgagac ggggcgtcgc
      601 gagttcctcg gcgccgagat tggtggtcat cacaacgatc gcctgcgaca tcgcgacggt
      661 gaacccgcgg ctgtcggtga cccgcccctc gtcgagtatc tgcaggagta tgccgagcac
      721 tttggggtgg gccttctcta tctcgtcgag caggagcatc tggtacggtt cgcgccggaa
      781 cgcctcggtg agtcgcccgc cgtcctcata cccggtgtag ccgggcggcg aaccgatgag
      841 gcgcgagacg gcgtgctgct cctggaactc gctcatgtcg aagatcacca tgcggtcggc
      901 gctgccgaag agatattcgg cgagccgccg tgccgtctcg gtcttgccga cacccgacgg
      961 accggcgaga agcaacgacg cgatcggccg tcgcgtgtcc cgtcgcacga atcggcgcgc
     1021 aagcagttgg acgatgcgtc gcttcgcctc ggcctgcccc tttatcgcgt catcgagccg
     1081 ttccgggagc gtctgcagga tgcgggtccc gtcgaccagc aagttctcca tcggtatgcg
     1141 cgatttcgcg ctgatgaact ccaagaggtc acgatccgtc gcctccttcc ggccggaacg
     1201 ctgaagcacc gaaccgagcg tgtcaaggag cgacagcacc ttgtcgggct ggctacgccc
     1261 cgccatgtag cgggtggaga gggcgatcgc ctgtttcacg aatgccggat cgtcgagccc
     1321 gacatggtgg aagaaggaaa gcgaccgggc cgattcctgc gcgatcgcga ggagcgcttc
     1381 tccttccggt tcctctatcg gcacgagcgt gaagcgacgc tccatcgccg gatccttggc
     1441 gatgg
//
LOCUS       DAOQUE010000616         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_124932_length_1438_cov_4.315980, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000616 DAOQUE010000000
VERSION     DAOQUE010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124932_length_1438_cov_4.315980"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PKH10_13555"
     CDS             <1..955
                     /locus_tag="PKH10_13555"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNT29191.1"
                     /translation="TAIDLLSAMDICKVSTNGSWGIVGTPTLTRASGSGAVDNRQIAV
                     MTQFGTHDSNVAIANDTMATLSSGRARDGNDPDATTALTYSYVTGTPPADFLSAHGGA
                     LPTTKDGCPSGSGANDSVMLTVKLKVPTNALSLSFNFRFFSQEYWEYTCSEYNDFFIA
                     LLDTGAEGIPADKNISFDSNDGYISVNTDQFFTVCEVKEGYTCPDGTDPLAGTGYDQQ
                     EEDTGGCTPGYHYVAGATQWLTTSAPVIPGETITLRFVIWDTSDQALDSLVLIDNFKW
                     SAEGTSGPVTFACWDTNKNGVCDVGTEDLSGDEKCSEFDCL"
     gene            complement(968..>1438)
                     /locus_tag="PKH10_13560"
     CDS             complement(968..>1438)
                     /locus_tag="PKH10_13560"
                     /inference="COORDINATES: protein motif:HMM:NF016503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERG2 family protein"
                     /protein_id="HNT29192.1"
                     /translation="IDMIDARWPGRIEKRQRWVLNSANGALGTMTILYASLSEYLIIF
                     GSPIGTEGHSGRYFATVHDFMVEGEMWVYVEGEIDRTVFRPGDHATLKARSAKGYRIE
                     GDSWMLEYAHGCIPAMLPAGVWDNLFSNLDFRSLWWTFWDYGRITVRELLRGKI"
BASE COUNT          286 a          431 c          429 g          292 t
ORIGIN      
        1 cacggcgatc gatcttttga gcgctatgga tatctgcaag gtatcgacga acggctcgtg
       61 gggcatagtc ggcaccccga cgctcacccg tgccagcggt agcggcgcgg tcgataaccg
      121 gcagatagcg gtcatgaccc agttcggcac tcacgattcg aatgtcgcga tcgccaacga
      181 cacgatggcg accctttcct cggggcgcgc ccgtgacggg aatgaccccg atgctaccac
      241 cgcgcttacg tactcgtatg ttaccggtac gccgcctgcc gatttcctct cggctcacgg
      301 tggtgcgttg ccgacgacaa aagacggatg cccgagcggt agcggcgcca acgattcggt
      361 catgctgacc gtcaaactca aggtgccgac caatgcgctt tccctatcgt tcaacttccg
      421 tttcttctct caggaatatt gggaatacac ttgttctgag tacaacgact tcttcatcgc
      481 gctgctcgat accggcgccg agggcatccc ggcagataag aacatctcct tcgattcgaa
      541 cgatgggtat atcagtgtga acacggacca gttctttacc gtgtgcgagg tcaaggaggg
      601 ctacacttgt cccgacggta ccgatccact ggcggggacc gggtacgacc aacaggaaga
      661 ggatactggt ggctgtaccc cgggatacca ctacgtcgcg ggggcgaccc aatggctcac
      721 cacctcggcg ccggtcatac cgggcgagac gataacgctc aggttcgtta tctgggacac
      781 ctcggatcag gcgctcgact cactcgtcct catcgacaac ttcaagtgga gcgctgaagg
      841 gacgagcggt ccggtgacct tcgcctgttg ggataccaac aagaatggtg tctgcgatgt
      901 cgggaccgag gatctttcgg gcgacgagaa gtgtagcgaa ttcgactgtc tgtaactttt
      961 cccctgatca gatcttgccg cgcagaagtt cgcgcaccgt gatgcggccg taatcccaga
     1021 aggtccacca gaggctgcgg aagtcgaggt tgctgaacag gttgtcccat acgccggccg
     1081 gtagcatcgc cgggatgcag ccgtgggcgt attcgagcat ccaactgtct ccttcgatgc
     1141 ggtaaccctt cgccgagcgg gctttgagcg tcgcgtggtc gcccggcctg aacacggtac
     1201 ggtctatctc tccttccaca tagacccaca tctcgccctc gaccatgaag tcgtggacag
     1261 tggcgaaata gcgccccgaa tggccctcgg tgccgatggg ggagccgaag atgatgaggt
     1321 attccgaaag cgatgcgtac agtatggtca tcgtgccgag cgcgccgttg gccgagttga
     1381 ggacccaccg ctggcgcttc tcgatgcgcc ccggccagcg ggcgtcgatc atgtcgat
//
LOCUS       DAOQUE010000617         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_125376_length_1435_cov_4.157971, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000617 DAOQUE010000000
VERSION     DAOQUE010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125376_length_1435_cov_4.157971"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PKH10_13565"
     CDS             <1..859
                     /locus_tag="PKH10_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNT29193.1"
                     /translation="YKQTLPPPSLAAEALTLAKEEEALEAGVNLVIHNGHGFPTLLTC
                     EQQGDDLDFTGAMAYALVNQTPLVFLSCACQAGQFEAPFTWHYTNSAGEQKERVFPDD
                     SAGELLVNAPNGGAILYLGNTTTGLGLAGGSQLIDEILRYSFAHDRPLIGDALRAARL
                     ALPQNDTFTPPVLTVPLPVVDPDSWAWTQKAVVLLGDGALPLWTVATPVSPLPLFSAE
                     KVAGGARLTITNIPAGSTARLHFCGGIYTVPTEESELTVTAPGDCLTAEAALASPATQ
                     YWFNSIPLL"
     gene            complement(871..>1435)
                     /locus_tag="PKH10_13570"
     CDS             complement(871..>1435)
                     /locus_tag="PKH10_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29194.1"
                     /translation="PKAVEFAKPDGIVIHCKNTFEPEAEGTIIADLEGRIKPRVVGIA
                     TEERVVMVRVRTGDATVMEAIRFAEKSGLRIKQISFHQSDDGAMDGSFIIPEKENYHL
                     DQVLEELTARFGAATTVRRDLAAISLVGAGITERHEFLGDAVAILRENGVGIGSIHTS
                     SFRISMLIDRGRLADAARMLYDRFITP"
BASE COUNT          258 a          487 c          430 g          260 t
ORIGIN      
        1 ctacaagcag accctgccac cgccgagtct cgccgccgag gcgttgacgc tcgccaagga
       61 ggaggaggcg ctcgaggcgg gggtgaacct cgtcatccac aacggccacg ggttcccgac
      121 cttgctcacc tgcgaacagc agggcgacga tctcgatttc accggcgcga tggcctatgc
      181 gctcgtcaac cagacgccgc tcgtctttct ctcctgcgcc tgtcaggccg ggcagttcga
      241 ggcgccgttc acctggcact acaccaacag cgccggggag cagaaagaac gcgtcttccc
      301 cgacgattcg gcgggcgaac tgctggtcaa cgccccgaac ggcggcgcga tactctatct
      361 cggcaatacc acgaccggcc tcggactcgc cggaggaagt caactgatcg acgagatatt
      421 gcgatattcc ttcgcccacg accgaccgct catcggcgac gcactcagag cggcacgact
      481 ggcattaccg cagaacgaca ccttcacccc gccggtactg accgtgccct tgccggtggt
      541 cgatcccgac agttgggcgt ggacgcagaa agcggtggtc ttgctcggcg acggcgcgct
      601 accgttgtgg accgtcgcga cgcccgtatc tccccttccc ctgttcagcg ccgaaaaggt
      661 cgcgggcggg gcacgcctca ccatcacgaa catcccggcc ggatcgacgg cgcgactcca
      721 tttctgcggg ggcatctata cggtcccgac ggaagagagc gaactgaccg ttaccgctcc
      781 gggcgactgc ctgaccgccg aagcggctct cgcctcgcca gcgacgcagt attggttcaa
      841 ttcgataccg ttactgtgac ctgcggatga tcacggggtg atgaaccggt cgtaaagcat
      901 gcgggccgca tcggcgagcc gcccgcgatc tatgagcatg ctgatacgga acgagctggt
      961 gtggatgctc ccgataccga cgccgttctc gcgcaggatc gcgaccgcat cgccgaggaa
     1021 ttcgtgccgc tcggtgatgc cggcgccgac gagtgaaatg gcggcgaggt cccggcgcac
     1081 ggtcgtcgcc gcgccgaaac gggccgtcag ttcctccagc acctgatcaa gatggtagtt
     1141 ctccttttca gggatgatga aactgccgtc catcgcgccg tcgtcactct gatggaacga
     1201 tatctgcttt atccgcaatc cgcttttttc ggcgaaccgg atcgcctcca tcaccgtagc
     1261 atccccggtg cggaccctga ccatgaccac ccgttcctcg gtcgcgatgc cgacgacccg
     1321 cggcttgatg cgtccctcaa gatcggcgat gatcgtccct tccgcctccg gctcgaaggt
     1381 gttcttgcag tggatgacga taccgtccgg tttggcgaac tcgaccgcct taggg
//
LOCUS       DAOQUE010000618         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_125697_length_1433_cov_3.172714, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000618 DAOQUE010000000
VERSION     DAOQUE010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125697_length_1433_cov_3.172714"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKH10_13575"
     CDS             <1..628
                     /locus_tag="PKH10_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNT29195.1"
                     /translation="KEGYLEPAAVQTLLDAAGIPRAGEAVVMKKADAVAAAKKLGYPV
                     VMKVVGPVHKSDVGGVVLGVKDDATVEKEFDRMMKIKDTTAILLQPMLSGIELFAGAK
                     FEEKFGHMVLCGLGGIFIEVLKDVNSGLAPLTKDEALAMIRSLKGYKILKGVRGQQAV
                     SEDKLADVVVRLSALVTAAPEIFEMDLNPLLGKGDQVVAVDARIRIVK"
     gene            complement(650..>1433)
                     /locus_tag="PKH10_13580"
     CDS             complement(650..>1433)
                     /locus_tag="PKH10_13580"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT29196.1"
                     /translation="PDDDGVPVPPGEMIDIPAGPFMMGCKEVGDELCYEDEYPYHQVT
                     LSAYKIGRYEVTVEEYRQCIADGACNNNGTYQHYRTDGSYSDCNVNRAGREKHPMNCV
                     TWHGAEAYCAYIGGRLPTEAEWEKAARGTDGRTYTWGNEPEPTCDLVAASDVTSEIGS
                     CENETGTMVVGSKPAGASPYGAQDMLGNVEEWVADWYDGGYYAVSPANDPQGLETPVP
                     GSEMRVKRGDSYIAYPTFLRVSNRDFVSPSYENYIIGFRCAQ"
BASE COUNT          280 a          445 c          439 g          269 t
ORIGIN      
        1 gaaggagggc tatctcgaac cggccgccgt ccagacgctc ctcgacgccg ccggcatccc
       61 gcgcgccgga gaggcggtgg tcatgaagaa ggcggacgcc gtcgccgccg ctaaaaaact
      121 gggctacccc gtcgtcatga aggtggtcgg cccagtccac aagtccgatg tgggcggggt
      181 cgtcctcggc gtgaaggacg acgcgacggt cgagaaggaa ttcgaccgga tgatgaagat
      241 caaggatacg accgccatcc tcctccagcc gatgctgtcg ggcatcgaac tgttcgccgg
      301 agcgaagttc gaggagaagt tcggtcacat ggtgctgtgc ggactcggcg gcatattcat
      361 cgaggtgctg aaggatgtga actccggtct cgcgccgctc accaaggacg aggcgctcgc
      421 gatgatacgg tcgctcaagg gctacaagat actgaaaggg gtccgcggcc agcaggcagt
      481 gtcggaagat aaactggccg atgtggtcgt gcggctctcg gcgctcgtca ccgccgcccc
      541 cgagatcttc gagatggacc tcaacccgct cctcggcaag ggcgatcagg tcgtcgcggt
      601 cgatgcgcgg atacgcatcg tcaagtaagg gatatcccct tcacacggcc tattgggcgc
      661 agcggaaacc gataatataa ttctcatagg aaggggagac aaaatcgcga tttgagaccc
      721 gtaaaaaggt aggataggcg atgtaactat caccgcgctt cacccgcatc tcggagcccg
      781 ggaccggggt ctccaacccc tgcgggtcgt tcgccggcga cacggcatag taacccccat
      841 cgtaccaatc cgccacccac tcttctacat tcccgagcat atcctgtgcg ccgtagggag
      901 atgcgccggc cggcttgctc cccaccacca tggtgcccgt ctcattctcg caactgccta
      961 tctcggaggt aacatcggag gccgccacca gatcgcaggt gggctccggt tcgttccccc
     1021 aggtataggt ccggccgtcg gtaccgcgcg ccgctttttc ccattcggcc tcggtgggaa
     1081 gccgaccgcc gatataggcg cagtaggcct ccgcgccgtg ccaggtgacg cagttcatcg
     1141 ggtgcttctc tcggcccgca cgattgacat tgcagtccga ataggaaccg tcggtgcggt
     1201 agtgctgata ggtgccgttg ttgttgcagg cgccgtcggc gatacactgc cggtattcct
     1261 ccacggtcac ctcgtatctg ccgatcttat aggccgacaa ggtcacctga tggtagggat
     1321 attcatcttc ataacagagt tcgtcgccca cctccttaca ccccatcatg aacggcccgg
     1381 cggggatatc gatcatttcg cccggcggga cgggaacgcc gtcgtcgtcg gga
//
LOCUS       DAOQUE010000619         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_125994_length_1431_cov_3.882267, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000619 DAOQUE010000000
VERSION     DAOQUE010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125994_length_1431_cov_3.882267"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PKH10_13585"
     CDS             complement(<1..388)
                     /locus_tag="PKH10_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29197.1"
                     /translation="MKKLMLATLLPLLLAAGEKELWEVTVPSWAVPNAAATAATLDIV
                     RMTEHDGAVVYHLWETAERVAPLAGLAFSSRVLPLALEGTEHYRDHDEMVAAMYVIEA
                     RLPLHAKVYDIGRSVEVERGHPDRHGR"
     gene            494..>1431
                     /locus_tag="PKH10_13590"
     CDS             494..>1431
                     /locus_tag="PKH10_13590"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNT29198.1"
                     /translation="MVKERNDIPTMLTFDDVLLVPAYSEVLPHETVIASKLTAKITLN
                     APFMSAAMDTVTEHKMAIAIAREGGIGIVHKNMAIEEQAREIKSVKRSESGVITQPIT
                     ITPERTLLDALDLMKYNGISGIPVTQKNKLVGIITNRDLRFETDLSKQVKEVMTTHLV
                     TVQDKCSVEEAKRLMHQHRIEKLLVVDKKGELTGLITTRDLENQEKFPNAAKDTFGRL
                     VVGAAISATDARLERAEALIEAGCDLLVLDSAHGHSKNIIARVRELKKRWPQVDVMAG
                     NIVTAEAAVALVEAGADVVKVGIGPGSICTTRVVSGV"
BASE COUNT          298 a          446 c          451 g          236 t
ORIGIN      
        1 ctcggccatg ccggtccggg tgaccgcgtt cgacctcgac cgaccggccg atgtcgtaca
       61 ccttcgcgtg caagggaagc cgcgcctcga tgacgtacat cgccgcgacc atctcgtcgt
      121 ggtcgcggta gtgttcggtc ccttcaagcg cgaggggcaa cacccgtgat gaaaaggcga
      181 gtccggcgag cggcgcgacc cgttcggccg tctcccacag gtgatacacg accgcgccgt
      241 cgtgctcggt catccgcacg atgtcgagcg tcgcggcggt cgcggcggcg ttcgggacgg
      301 cccacgacgg gacggtcacc tcccagagtt ccttctctcc cgcggcaagc aggagcggca
      361 gtagggtggc gagcatgagt ttcttcatgc ggcgattgta aagagcgcgg cgacaagagt
      421 caacatttag tattctattg acttgctcgg ccggtttcat acaatgccgc caaacggtgt
      481 ggtggaggcc acgatggtga aagaacgcaa cgatatcccg acgatgctca ccttcgacga
      541 tgtcctgctc gtgccggctt attccgaggt tctgccgcac gagacggtca tcgcctcgaa
      601 actgacggcc aagataacgc tcaacgcccc cttcatgagc gccgcgatgg acacggtgac
      661 cgagcacaaa atggccatcg cgatagcgcg cgagggaggc atcggcattg tccacaagaa
      721 catggccatc gaggagcagg cgcgcgagat aaagtcggtc aaacgctccg aaagcggcgt
      781 tatcacccag cccatcacca tcaccccaga acggacgctc ctcgacgcgc tcgacctgat
      841 gaagtacaac ggcatctccg gcataccggt cacgcagaag aacaaactgg tcggcatcat
      901 caccaaccgc gatctccgct ttgagaccga cctgtccaag caggtgaagg aggtgatgac
      961 cactcatctg gtcacggtgc aggacaaatg ctcggtcgag gaggccaagc ggctcatgca
     1021 ccagcaccgc atcgagaaac tgctcgtggt cgataagaaa ggggaactca ccgggctcat
     1081 cacgacgcgc gatcttgaga atcaggagaa gttcccgaac gccgcgaagg ataccttcgg
     1141 ccgtctggtc gtcggcgccg ccatcagcgc caccgacgcc cggctcgaac gggccgaggc
     1201 gctcatcgag gctgggtgcg acctgctggt gctcgactcg gcgcacggcc attccaagaa
     1261 catcatcgcg cgggtcagag aactcaaaaa acggtggccg caggtcgatg tgatggcggg
     1321 caacatcgtc accgccgagg cggcggtcgc gcttgtcgag gcgggcgccg atgtggtcaa
     1381 ggtcggcatc ggtcccgggt cgatatgcac cacgcgggtc gtgtcggggg t
//
LOCUS       DAOQUE010000620         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_126282_length_1429_cov_3.422125, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000620 DAOQUE010000000
VERSION     DAOQUE010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126282_length_1429_cov_3.422125"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PKH10_13595"
     CDS             complement(<1..691)
                     /locus_tag="PKH10_13595"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNT29199.1"
                     /translation="MTKYLMLLAAMSLLIACGDEGTKGPTMGAKGGDCYGNGTCDGDL
                     VCMSNVCVDPTETPDKDTVVNDTATNDSTVTDTGQPDLPDLIGDEDSDVEDPDVDYGT
                     GTPGEMVDIPAGEFQMGCNEGVDTECSEDEKPYHAVILSPYKIGKYEVTGGEYQQCVT
                     SGACNNSNDEEPHYRTNSDNLFCNLGAAGKEDHPMQCVTWYGAKAYCKWIGGRLPSEA
                     EWEKAARGTDGR"
     gene            complement(708..>1429)
                     /locus_tag="PKH10_13600"
     CDS             complement(708..>1429)
                     /locus_tag="PKH10_13600"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT29200.1"
                     /translation="SAVDDQCEDDEYPYHAVTLSAYKIGKYEVTVGEYQQCVTNGACN
                     NNGDHTHYYTNSDESYCNYGDEGKGSHPMQCVTRSGAQAYCEWIGGRLPTEAEWEKAA
                     RGTDGRKYPWGSEPTVSCDYAVMAPYNESTGYYDFGCGTNGTMPVGSKSNGVSPYGAY
                     DMSGNVMEWLNDWYGGDYYETTPTNTPAGPETGASRVVRGGSWKSHYDFALRASRRDY
                     RYPVDYSRDSDIGFRCVKDAE"
BASE COUNT          234 a          449 c          378 g          368 t
ORIGIN      
        1 tccgtccatc ggtgccccgc gccgctttct cccattcggc ctcgctggga agtcgcccgc
       61 cgatccactt gcaataggcc tttgcgccgt accatgtcac gcattgcatg ggatggtcct
      121 cttttcccgc cgcgccaaga ttgcagaata aattatcgct attggttcgg taatgtggtt
      181 cctcgtcgtt gctattattg caggccccac tggtcacgca ctgttgatat tcgccgccgg
      241 tcacttcata tttgccgatc ttgtaggggc tgagtatcac agcgtggtac ggtttttcgt
      301 cttcactaca ctccgtatcc accccttcat tacagcccat ctggaattcc ccggcgggga
      361 tatccaccat ttcgcccggt gtgccggtgc cgtagtcgac atcggggtct tcaacatcgc
      421 tatcttcgtc tccaatcaga tcaggtaggt caggctgtcc ggtgtcggtc accgttgaat
      481 cgttcgtcgc cgtgtcgttg accaccgtgt ccttgtccgg cgtttcggtc ggatcaacgc
      541 agacattgga catgcagacg aggtcgccgt cgcaggtgcc gttcccgtaa cagtccccac
      601 ccttcgcccc catcgtcggg cctttcgtcc cctcatcgcc acaggcgatc agcagggaca
      661 tcgccgcaag gagcatcagg tatttggtca caggggcctc ccttttttta ttcagcatcc
      721 ttcacgcacc ggaacccgat gtcgctgtcg cggctgtagt cgactgggta gcggtagtcg
      781 cgccgggacg cgcgcagggc gaagtcgtag tggcttttcc acgacccgcc acgcaccacg
      841 cgggaagcac cagtttctgg ccccgcaggg gtgtttgtag gcgtcgtttc gtaataatct
      901 ccaccatacc agtcgttcag ccattccatc acattgccgc tcatgtcata ggcgccgtag
      961 ggggatacgc cgttcgactt gctccccacc ggcatggttc cgttggtgcc gcaaccgaaa
     1021 tcatagtagc cggtcgactc attgtatggc gccatgactg cataatcgca cgatacggtc
     1081 ggttcgctcc cccatgggta tttccgtccg tcggtgcctc tggccgcttt ttcccattcg
     1141 gcctcggtcg gcaggcgacc acctatccat tcgcaatagg cctgggcacc agaccgtgtc
     1201 acgcactgca tgggatggct ccccttcccc tcatcgccgt agttgcaata agactcatcg
     1261 ctgttggtgt agtagtgtgt gtggtccccg ttattgttgc aggcaccgtt ggtgacgcat
     1321 tgctgatatt cgcccactgt aacttcgtat ttgcctattt tataggcgct gagcgtcacg
     1381 gcgtggtagg ggtactcgtc atcctcgcat tgatcgtcca ccgctgaat
//
LOCUS       DAOQUE010000621         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_126989_length_1424_cov_3.899927, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000621 DAOQUE010000000
VERSION     DAOQUE010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126989_length_1424_cov_3.899927"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PKH10_13605"
     CDS             <1..980
                     /locus_tag="PKH10_13605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT29201.1"
                     /translation="SVWKGHDAQTMAREIEEIKKRSTADQIRVKAAEYFYAYLMIERL
                     DALLADTVKQLDRYETQAQNFVDAGLTDKRAVLKVKLEKARLEKERQNVEGNRTLLKR
                     ALALLMNRDMESFAPFSESPDEIAPVPSLDRLLALQDEHRAEIAMLDRADRVAATLQD
                     IAIQPLVPTIALTGGYKHNFDATTFSPEGTFFVGAVLSWDFGFDTMKQINSFKKARGE
                     KTKMLLANIDVRKQLRLQVTQLYTDLMVKEKEIAIATAEREVAAENLRIEESKYQEKL
                     TTETDLLAASLQERQAKTSYITAIFQYKIALQKLAGTIGVPATALNGNQ"
     gene            997..>1424
                     /locus_tag="PKH10_13610"
     CDS             997..>1424
                     /locus_tag="PKH10_13610"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNT29202.1"
                     /translation="MLRSFIVISISTLISFLAAAGCSSGNAGEQESAAVQPRREVTKD
                     VRLVEPLRKRTAVIIRESVTLAPEEKGAVAAEVGGTVLKFHVEENQRVKKGQIVATLD
                     ATDYILGLEQARAGVAALEAQYAGLGNDYQRIKGLYEKGAT"
BASE COUNT          342 a          396 c          449 g          237 t
ORIGIN      
        1 ggagcgtctg gaaagggcat gatgcccaga cgatggcgcg ggagatagag gagataaaga
       61 agaggtccac cgccgaccag atccgggtga aggcggccga atacttttat gcatatctta
      121 tgatcgagcg gctcgatgcc ctgctcgccg atacggtcaa gcaactcgac cgctatgaga
      181 cgcaggcgca gaatttcgtc gatgccgggc tgaccgacaa gcgggcggtg ctcaaggtga
      241 aactcgagaa ggcgcggctt gaaaaggaac gacagaatgt cgaaggcaac cggacgctcc
      301 tgaaacgggc gcttgccctg ctcatgaatc gcgatatgga aagtttcgcg cccttctcgg
      361 aaagcccgga tgagatcgcg ccggtgcctt cgcttgaccg cctgctggcc ttgcaggacg
      421 aacatcgtgc cgagatagcg atgcttgacc gtgccgaccg ggtcgccgcg accctgcagg
      481 atatcgccat ccagccgctc gtcccgacga tagcgcttac cgggggctac aagcacaatt
      541 tcgacgcgac caccttcagc cccgagggga ccttcttcgt tggcgcggtc ctgtcgtggg
      601 atttcggttt cgacacgatg aagcagataa attccttcaa aaaggcgcgc ggcgaaaaga
      661 cgaagatgct gctggccaac atcgatgtcc gcaagcagtt gcgcctgcag gtgacccaac
      721 tgtataccga ccttatggtc aaagaaaagg agatcgccat cgcgaccgcg gaacgggagg
      781 tcgccgccga gaacctgcgc atcgaagagt cgaagtatca ggagaaactg acgaccgaga
      841 ccgacctgct cgcggcaagc ctgcaggagc ggcaggcgaa gacctcctac atcaccgcca
      901 tattccagta caagatagcg ctccagaaac tcgcggggac catcggcgtt ccggcgaccg
      961 cgctgaacgg caatcagtaa aacggagaga tatagtatgt tgagatcctt tattgtcatt
     1021 tcgatatcga cgctgatatc tttcctcgcc gccgccggat gcagtagcgg caacgccgga
     1081 gaacaggaga gtgcggcggt ccaaccacgc agggaggtga ccaaggatgt tcgactggtc
     1141 gagccgctca gaaagaggac ggcggtcatc atcagagaat cggtgacgct cgctcccgag
     1201 gaaaagggcg cggtcgccgc cgaggtgggg ggcacggtgc tcaaatttca cgttgaggag
     1261 aatcagcggg tgaaaaaagg gcagatcgtc gcgacgcttg acgccaccga ctacatcctc
     1321 ggtctcgagc aggcgcgggc gggagtggcg gcgctcgagg cgcaatatgc cggactcggc
     1381 aacgactatc agcggatcaa aggtctctat gaaaaagggg ccac
//
LOCUS       DAOQUE010000622         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_126995_length_1424_cov_3.624543, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000622 DAOQUE010000000
VERSION     DAOQUE010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126995_length_1424_cov_3.624543"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PKH10_13615"
     CDS             complement(<1..832)
                     /locus_tag="PKH10_13615"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNT29203.1"
                     /translation="MVRATRFLRIKEIGTILAKYDLIPWVPTVAQRLLGYVGTGESNK
                     RSRAMPVGKKLRFAFEELGTTYIKLGQLLSLRKDVIPEEITEELKQLQDRVSPIPFDE
                     MRPIIERNICAPIEEIFSQFDEEPLAAASISQVYHAVLKKNGAEVVVKVRKPGIVDKM
                     RSDLEMLKWLAEIMQNNSKLGEHFDFVGIIDEFFSTMEMELDFTHELRNTRRFARNFS
                     GAEWDWLVFPKIYDEYCNKYLLVMEYIKGRKLDQLGDPAFTIDRALLAKNGARSFMKQ
                     V"
     gene            complement(842..>1424)
                     /locus_tag="PKH10_13620"
     CDS             complement(842..>1424)
                     /locus_tag="PKH10_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29204.1"
                     /translation="ALLESFDKLAGAGATGPDSAAFNAYLNMLRNIRFRFGDIPATER
                     LGCSPAAGDILRRHFALHRERTALPNFFSNYPLLFVCPPDERDIRITISQIHTHKIRG
                     ADIVLIAEENGALRKAVFGRPTGLDQYYAEYIAVPATGDRNLFTFEAAAILQTLAFKM
                     SVEKMKYLNRNRIENHGVHPDVPKNVSKSITVD"
BASE COUNT          261 a          367 c          475 g          321 t
ORIGIN      
        1 acacctgctt catgaacgag cgtgcgccgt tcttggccag aagcgcgcga tctatggtga
       61 aggcggggtc gccgagctgg tcgagtttgc ggcccttgat gtattccatc acgagcaggt
      121 acttgttgca gtactcgtcg tatatcttgg ggaatacgag ccagtcccac tccgcgccgg
      181 agaagttgcg cgcgaaccgg cgggtgttgc ggagttcgtg ggtgaaatcg agttccatct
      241 ccatcgtgga gaagaactcg tctatgatgc cgacgaaatc gaaatgttcg cccagtttgc
      301 tgttgttctg catgatctcg gcgagccatt tgagcatttc gaggtcgcta cgcatcttgt
      361 ccacgatgcc cggtttacgg accttgacga ccacttcggc gccgttcttc ttgaggaccg
      421 cgtgatagac ctgtgatatt gatgcggcgg caagcggctc ctcgtcgaat tgcgagaata
      481 tctcttcgat gggggcgcag atgttgcgct cgatgatggg gcgcatctcg tcgaacggga
      541 tcgggctgac gcgatcctgc aattgtttga gttcctcggt tatctcttcg ggaatgacat
      601 ccttgcgcag ggagagcaac tggccgagtt tgatgtaggt ggtaccgagt tcctcgaacg
      661 cgaagcgcag tttcttcccc accggcatgg cgcgggaccg cttgttcgat tcgccggtcc
      721 cgacatagcc gagcagtcgc tgggcgacgg tggggaccca cgggatgaga tcgtacttgg
      781 caagtatcgt gccgatctcc tttatgcgca ggaagcgggt ggctcggacc atggtgtgcc
      841 tctaatcaac ggttatcgac ttggacacat tcttagggac atcggggtgc acgccgtgat
      901 tctcgatgcg gttgcggttc aggtatttca tcttttccac cgacatcttg aacgcaagcg
      961 tctggaggat ggccgccgct tcgaaggtga agagattgcg gtcgccggtc gccggtaccg
     1021 cgatatattc ggcgtaatac tggtcgagtc cggtcggacg gccgaacacc gccttgcgga
     1081 gcgccccgtt ctcttcggcg atgagtacga tgtcggcccc gcggatcttg tgggtgtgta
     1141 tctgggagat ggtgatacgg atatcgcgtt cgtcgggggg gcagacgaag agcagggggt
     1201 agttcgagaa gaagttgggg agcgccgtcc gttcgcgatg gagcgcgaaa tgacggcgca
     1261 ggatatctcc ggccgcgggg gagcagccga ggcgctcggt cgccgggatg tcgccgaaac
     1321 ggaagcggat gttgcgcaac atattgagat aggcgttgaa agcagcggaa tcaggtccgg
     1381 tcgcccccgc tccggcgagt ttgtcgaacg actcgaggag cgcg
//
LOCUS       DAOQUE010000623         1422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_127308_length_1422_cov_3.506218, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000623 DAOQUE010000000
VERSION     DAOQUE010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127308_length_1422_cov_3.506218"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PKH10_13625"
     CDS             <1..494
                     /locus_tag="PKH10_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29205.1"
                     /translation="GIAQAIIHNPEVVIMDEPMSGLDPLGRHEVKEIIRELRKEGKAV
                     LFSSHILADIEDLSDRVLIIEKGTRRFFGPLADIVLAQDIRWRVSFSAPEQEHPLIFS
                     SFKGAHLADGRFSLTLNTEQEMNALLPSLIERRYTIVAAGPVYPTLEEIVFHMKDREA
                     PHG"
     gene            487..1257
                     /locus_tag="PKH10_13630"
     CDS             487..1257
                     /locus_tag="PKH10_13630"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT29206.1"
                     /translation="MGKILTIARYTFLEMVRHKVLYAFVFFAFFILLLGVAITQMTIG
                     DEVDIISDIGLGTIEIFATVVVIFMGISIINKEITLRTLHPLLARPISRPQYVLGKFA
                     GMVILAVVGIAFMSLVLWLMLYFYGGAGRMSAYLPAIYTIFLQTTLLVSVAVLCSSFV
                     EPAIGALFTIGFYLIGATSYSLAFILDMNLSDRIKRAVSVMRLILPDFSYFNIKDNIV
                     YGRGLESISLSYATLYAVAGIVIILSLAVLLFDRKELN"
BASE COUNT          272 a          472 c          364 g          314 t
ORIGIN      
        1 tcggcatagc gcaggccatc atccataacc ccgaggtcgt catcatggac gagccgatga
       61 gcggactcga tccgctcggt cgtcatgagg tcaaggagat catccgcgaa cttcgcaagg
      121 agggcaaggc ggtcctcttc tcatcccaca tactcgccga catcgaagat ctttccgacc
      181 gtgtgctcat catcgaaaaa gggacccgcc gctttttcgg gccgcttgcc gatatcgttc
      241 tcgcgcagga catccgctgg cgcgtttcct tctccgcccc cgaacaggaa caccccctga
      301 tattctcttc cttcaagggt gcgcacctcg ccgacggccg tttctctctc accctgaaca
      361 ccgaacagga gatgaacgcc cttctcccct ctctcatcga gcggcgctac accatcgtgg
      421 cggcggggcc ggtctatccc acgctcgagg agatcgtgtt ccacatgaag gaccgggagg
      481 cgccccatgg gtaagatact gaccatcgcc cgctacacct ttctcgagat ggtacggcac
      541 aaggtgctgt acgccttcgt gttcttcgcg ttcttcatcc tcctgctcgg cgtcgccatc
      601 acccagatga ccatcggcga cgaggtcgac atcatatcgg acatcggact cggcaccatc
      661 gagatattcg ctaccgtcgt cgtgatattc atgggcatct ccatcatcaa caaggagata
      721 acgctccgta cgctccatcc gctactcgcc cgccccatca gccgcccgca gtatgtgctg
      781 ggcaaattcg ccggtatggt cattctcgcg gtcgtcggca tcgcattcat gagtctcgtg
      841 ctctggctca tgctctattt ctacggcggc gcgggtcgca tgtccgcgta tctgccggcc
      901 atctacacga tattcctcca gaccacactc ctcgtatcgg tggcggtgct gtgcagttcc
      961 ttcgtcgagc cggccatcgg cgcgctattc acgatagggt tctacctcat cggtgccaca
     1021 tcctacagtc tcgcgttcat ccttgacatg aacctgagcg accggataaa gagggccgtg
     1081 tcggtcatgc ggcttatcct tcccgacttc tcctacttca acatcaagga caacatcgtg
     1141 tacggcagag gactcgaaag catcagtctc tcctacgcca cgctctacgc cgtcgccggc
     1201 atcgtcatca ttctttctct cgcggtgctc ctgttcgatc gcaaggaact caactgatgt
     1261 ccctgacgat actggtccgc tacgacgaga taggcaccaa gggcaagaac cgtgcatggt
     1321 tcgaggacct gctcgtgagg aacatcgtac ggggtctcgg tcccgactgc gccgcccgaa
     1381 aattccgcgg ccgcatcgaa ctgacggtgg tcggcgacat ga
//
LOCUS       DAOQUE010000624         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_128829_length_1412_cov_4.362564, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000624 DAOQUE010000000
VERSION     DAOQUE010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128829_length_1412_cov_4.362564"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PKH10_13635"
     CDS             complement(<1..700)
                     /locus_tag="PKH10_13635"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT29207.1"
                     /translation="MRILVSLAFVLVASSLCAEAGNEAPPSPAQLAAFNEKKCTGGDV
                     DACMFLAENYYYGKELGKNPAKARIFYRHACTKGHADACSNLGAMLESGEGGGRDWNG
                     AETHYKKSCNGGSGLGCNNLAVLYHQRSRKEPKFLSKAADAYNSACAMQYEGACAQAE
                     GLQKFLETDPAKKLEGRQIACSGKDDLDACHQAGTMHYLGEGTPRSVAKAAEYFKKAC
                     DGGVAAACYNIAIMM"
     gene            851..>1412
                     /locus_tag="PKH10_13640"
     CDS             851..>1412
                     /locus_tag="PKH10_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29208.1"
                     /translation="MKKMLGLLVVTAFVLLTACDGTTKKKVVDDDTVGGDTIVEADGD
                     ATGTDADLAATCGNGSIDLGEPCDGDMKNCVEIDPVKYTGGKAKCRADCAGYDTITCD
                     ENPAVCGNGIVEGAEKCDGTIDNCVDIDPLMFVAGKARCKADCTGWDIETCESSGDYC
                     GDGEKSETEFCDGDAKACAELNPSLYE"
BASE COUNT          287 a          411 c          448 g          266 t
ORIGIN      
        1 ccatcataat ggctatgttg tagcaggccg cggcgacacc accgtcgcac gccttcttga
       61 aatactccgc cgccttcgcg acactgcgcg gggtcccctc gccgaggtaa tgcatagtcc
      121 ccgcctgatg acaggcatcg agatcgtcct taccgctaca ggcgatctgc cgtccttcga
      181 gttttttcgc cggatcggtc tcgaggaatt tctgcagacc ctccgcctgt gcgcaggccc
      241 cttcatactg catcgcgcag gccgagttgt aggcatcggc ggcctttgag aggaacttgg
      301 gttccttgcg tgaacgctga tggtaaagga ccgcgagatt gttgcacccg agtcccgagc
      361 cgccgttgca cgactttttg tagtgcgtct cggcgccgtt ccagtcgcgg ccgccacctt
      421 cgccgctttc cagcatcgcg ccgagattcg agcaggcatc ggcatggcct ttggtgcagg
      481 cgtgtcggta aaagatgcgt gccttggcag gattctttcc cagttccttc ccgtaataat
      541 agttctcggc gaggaacatg caggcgtcca catcgccgcc ggtgcatttc ttctcgttga
      601 acgcggcaag ttgcgccggg ctcggcggcg cctcattgcc cgcctcggcg cacagggacg
      661 aggcgacaag aacgaaggcg agcgaaacga ggatgcgcat ggtgtccccc gtgttttcac
      721 gatacgggac cgatactaac aacgcccggt gccggtgtca ataccgcggc aaaaagttga
      781 ccgtgcccct cttttccggc atactggcgc cgtctcaagg acggggtcat agaaaaggag
      841 gaacaacgac atgaaaaaga tgctgggcct gctggtcgtt acggcgttcg tactgcttac
      901 cgcgtgcgac ggcaccacga agaaaaaggt ggtggacgac gatacggtcg gcggcgatac
      961 gatcgtcgag gccgacggtg atgcgaccgg gacggatgcc gatctcgcgg cgacctgcgg
     1021 caacgggagc atcgatctcg gcgagccatg cgacggcgac atgaagaact gcgtagagat
     1081 agatccggtg aagtatactg gaggcaaggc gaaatgcagg gccgactgtg ccgggtacga
     1141 cacgatcacc tgcgacgaga acccggccgt atgcggcaac ggcatcgtcg aaggagccga
     1201 gaagtgcgac ggcacgatag acaactgcgt ggacatcgat ccgctgatgt tcgtcgccgg
     1261 caaggcgcgg tgcaaggccg actgcaccgg ctgggatatc gagacctgcg agagttccgg
     1321 cgattattgc ggcgacggcg aaaagagtga gaccgagttc tgcgacggtg acgcgaaggc
     1381 ctgcgccgag ctcaatccat cgctttatga ga
//
LOCUS       DAOQUE010000625         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_129283_length_1409_cov_4.396603, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000625 DAOQUE010000000
VERSION     DAOQUE010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129283_length_1409_cov_4.396603"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1409
                     /locus_tag="PKH10_13645"
     CDS             <1..>1409
                     /locus_tag="PKH10_13645"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29209.1"
                     /translation="DDDGDGISNGVEGCGDNDLDGLPNWLDADSDGDGILDSQECPEQ
                     PCQNSDQDTSPDFLDKDSDNDGLSDKEEITLGTDPYDKDTDGDDSDDLAEIVYGSDPK
                     DPNDTIPPGLFFVVLPYNAPEDVTRTLEFSTDFSTVDIAIQLDLSGSMEQEKANLQSE
                     IKDKIIDGIPAKVPGLDVATGFVHFMDMENNRMFVVDHMISKNADSVKGAVDGLPDTY
                     GGTEPHQEVLYQTATGLGLNANFFLTPVGGFATNLKIEPADCTGELGSVGGLCFRELA
                     LKIFIMITDEAFPQFGIKGTPSCTITSSADGCWDPNFHGASTEDAINAMNGINAKFIG
                     VDSAFSCSQYDDQYNCIGTSSPSDVAKEDFTIVAEGTGSLDGNGNPFLYHTESFDGSG
                     LSDQIAEAVKELTTYIEKDITTSAESDEQCGGISAAAFVSSAEPNKSDPDGNYENKDE
                     TTFYKVKPSTLVYFDIHFH"
BASE COUNT          333 a          409 c          408 g          259 t
ORIGIN      
        1 cgacgacgac ggcgacggga tatccaacgg cgtggaaggg tgcggcgaca acgatctgga
       61 cggcctgccc aactggctcg atgccgattc ggacggcgac ggcatactcg acagtcagga
      121 gtgccccgaa cagccgtgcc agaacagtga tcaggatacc tctcccgatt tcctcgacaa
      181 ggacagcgac aacgatggac tctccgacaa ggaggagatc acgctcggta ccgacccgta
      241 tgacaaggac accgatggcg acgattcgga cgacctcgcc gagatcgtgt atggctccga
      301 cccgaaggac cccaacgaca ccatccctcc ggggctcttc tttgtcgtgc ttccctacaa
      361 tgcgccggag gatgtgaccc gcacgctcga gttctcgacc gatttctcga cggtcgacat
      421 cgcgatacaa ctcgacctgt cggggtcgat ggaacaggaa aaagccaatc tgcagagcga
      481 gataaaggac aagatcatcg acgggatacc cgcgaaggtg ccggggctcg atgtcgcgac
      541 cggctttgtc cacttcatgg acatggagaa taaccgtatg ttcgtggtcg atcacatgat
      601 cagcaagaac gccgactcgg tgaaaggcgc ggtcgacggt ctgcccgata cctacggtgg
      661 caccgaaccg catcaggagg tcctgtatca gacggcgacc ggtctgggac tcaacgcgaa
      721 tttctttttg acgccggtcg gaggtttcgc cacgaacctg aagatagagc ccgctgactg
      781 caccggggaa ttgggctcgg tcggcggtct ttgcttccgc gaactggcgc tcaagatatt
      841 catcatgata accgacgagg cgttcccgca gttcggcatc aagggaacgc cgagttgcac
      901 gataacctcc agcgccgacg gatgctggga tccaaacttc cacggtgctt cgaccgaaga
      961 tgcgatcaac gcgatgaacg gcatcaacgc gaaattcatc ggggtcgatt cggccttctc
     1021 ctgctcgcaa tatgacgacc agtacaattg tatcggcacc tcttccccga gcgatgtcgc
     1081 caaagaggat ttcacgatcg tcgccgaggg gaccggatcg cttgacggga acgggaaccc
     1141 gttcctgtat cacacggaga gttttgacgg gtcaggcctt tcggatcaga tcgcagaggc
     1201 ggtgaaggaa ttgaccacct acatcgaaaa ggacatcacg accagcgccg agtcggatga
     1261 acagtgcggt ggcatctcgg cggcggcatt cgtttcttcg gcggaaccga acaaatccga
     1321 tcccgacggg aactatgaga ataaggacga gacgacattc tacaaggtca agccctcgac
     1381 gctcgtctat ttcgacatcc atttccaca
//
LOCUS       DAOQUE010000626         1405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_129960_length_1405_cov_2.697778, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000626 DAOQUE010000000
VERSION     DAOQUE010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129960_length_1405_cov_2.697778"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PKH10_13650"
     CDS             <1..870
                     /locus_tag="PKH10_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24B family metallopeptidase"
                     /protein_id="HNT29210.1"
                     /translation="NETEIAGMRMAHLRDGVAMVRFLAWLDMALSAMRVTEISAAVQI
                     EEIRRRGERYVGPSFAPIVGYGEHGAIVHYSATPDTDAEIQREGLLLIDSGGQYLDGT
                     TDITRTIAVGTPTDGMKRHFTLVLKGHIGVASAVFPAGTAGMFLDPFARRALWDDRLN
                     YGHGTGHGVGAYLSVHEGPQGISPRVNETRLEPGMILSNEPGYYRERTYGIRTENLVL
                     VKSVGENEGANWLGFETITLCPIDLDLVDPAILSPGERQWLNDYHRTVRESLLPFLAD
                     EEKEWLTRETREI"
     gene            904..>1405
                     /locus_tag="PKH10_13655"
     CDS             904..>1405
                     /locus_tag="PKH10_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNT29211.1"
                     /translation="MCGVVSILYGEQTEFLGREASDLLKKLEYRGYDSTGGAFISDDG
                     AVILRKKVGAPSRVIEKLEIDRLPGKRFIGQVRWATYGSVTDQNAQPHEVSCRAHLVG
                     AHNGNISNTDALKEFLTHHGHTVLSDNDGEMLVHLVEHHYAIRTAGERLGEKERIAAM
                     KDAIRTA"
BASE COUNT          287 a          465 c          429 g          224 t
ORIGIN      
        1 aacgagaccg agatcgccgg gatgcgcatg gcccatctgc gcgacggcgt cgcgatggtg
       61 cgtttcctcg cgtggctcga catggccctc tccgcgatgc gggtgaccga gatatcggcc
      121 gccgtgcaga tagaggagat ccggcggcgc ggcgaacgct atgtcggccc gtcgttcgcc
      181 cccatcgtcg gctacggcga acacggcgcc atcgtccact acagcgccac ccccgatacc
      241 gatgccgaga tacagcgcga ggggttgctc ctgatagatt caggcggcca gtatctcgac
      301 ggcaccaccg acatcacccg cacgatagcg gtgggcacgc cgaccgatgg gatgaaacgg
      361 cacttcacgc tcgtcctcaa gggacacatc ggcgtcgcat cggccgtatt cccggccggt
      421 accgcaggga tgttcctcga tccgttcgcg cggcgcgccc tctgggacga ccggctcaac
      481 tacggtcacg gcaccggcca cggcgtcggc gcgtatcttt cggtccatga aggaccgcag
      541 ggcatcagcc cccgcgtgaa cgaaacgaga ttggagccgg gcatgatact ctccaacgag
      601 ccagggtact atcgcgaaag aacttacggt atccgcaccg agaacctcgt gctcgtgaag
      661 agcgtcgggg agaatgaagg ggcgaactgg ctcggcttcg aaacgataac cctctgcccg
      721 atagacctcg atctcgtcga ccccgcgatc ctgtcacccg gtgagaggca gtggctcaac
      781 gattaccacc ggacggtgcg cgagtcgctc ctgccgttcc tggccgacga ggaaaaggag
      841 tggctgaccc gtgaaacgag agagatataa cccgaccgcc cttaccagaa cggagtgacg
      901 atcatgtgtg gcgtcgtatc catcctctac ggtgaacaga ccgaattcct cgggcgcgag
      961 gcgtccgatc tcctcaagaa actcgagtac cgcggttacg actcgaccgg cggcgccttt
     1021 atctccgacg acggcgcggt catactgcgc aaaaaggtcg gcgccccctc gcgggtcatc
     1081 gagaaactcg agatagacag actgccgggg aagcgtttca tcggccaggt ccgctgggcc
     1141 acctacggct cggtcaccga ccagaacgcc cagccgcacg aggtctcgtg ccgcgcccat
     1201 ctggtcggcg cccacaacgg caacatatcg aacaccgacg cgctcaagga gttcctcacc
     1261 caccatgggc atacggtgct ctcggacaac gacggcgaga tgcttgttca tctcgtcgaa
     1321 catcattacg ccatccggac ggccggcgag cgcctcggcg agaaggaacg gatcgcggcg
     1381 atgaaggatg ccatccgtac cgccc
//
LOCUS       DAOQUE010000627         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_130068_length_1404_cov_4.118606, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000627 DAOQUE010000000
VERSION     DAOQUE010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130068_length_1404_cov_4.118606"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PKH10_13660"
     CDS             <1..1046
                     /locus_tag="PKH10_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNT29212.1"
                     /translation="YGVFIDLGGIDGLLHITDMSWGRVNHPSEMFKIGDEVEVKVLKF
                     DRDAERVSLGLKQISDDPWQYASERYNVGKVVRGKIVALEDYGAFVELERGIEGLIHI
                     SEMSWTRRVKHPSKMVAIGDDVEVKILEIDVDNRRISLGMKQTEPNPWEQLKDKYPVG
                     TMVKGVIRNITDFGLFVGVEDGIDGLVRTSDITWDRKLKHPSEYYKKGDEIEAMVLNI
                     DSEKQRFSLGVKQMSHDPWKMFRESYKENDKIEAEVVKTTDFGIFVKVFDTIEGLVHK
                     TEFEDPNKNFSIGDKIEVIIKSISDTERKVSLSIKAISEAESRQNMEEAKAKTGEVPE
                     RLGSIGDAFKNQQ"
     gene            1147..>1404
                     /locus_tag="PKH10_13665"
     CDS             1147..>1404
                     /locus_tag="PKH10_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNT29213.1"
                     /translation="MPVNLKGRSFLTLKDFTPEEIRYLLDLSRDLKAKKRAGIKGDLL
                     EGKNIVLLFEKASTRTRCAFEVAVLDEGGHVTFLGTNDSQMG"
BASE COUNT          347 a          394 c          424 g          239 t
ORIGIN      
        1 actacggcgt cttcatcgac ctcggcggca tcgacggcct gctgcacatc accgacatga
       61 gttggggtcg cgtcaatcat ccgagcgaga tgttcaagat aggcgacgag gtcgaggtca
      121 aggtgctcaa gttcgaccgt gacgcggagc gcgtgagcct cgggctcaag cagatatcgg
      181 acgacccgtg gcagtacgcc tcggagcgct acaatgtcgg caaagtggtc cgcggcaaga
      241 tcgtggcgct ggaggattac ggcgcgttcg tcgaactgga gcgcggcatc gaggggctca
      301 tccacatcag cgagatgagt tggacccgcc gggtcaagca cccgagcaag atggtcgcga
      361 taggcgacga tgtcgaggtg aagatactcg agatcgatgt cgacaaccgt cgcatcagtc
      421 tcggcatgaa gcagaccgag cccaacccgt gggagcagtt gaaggataag tatccggtcg
      481 gcaccatggt caagggcgtc atccgcaaca tcaccgattt cggcctgttc gtcggcgtcg
      541 aggacggcat cgacgggctc gtccgcacga gcgacatcac ctgggaccgg aaactcaagc
      601 acccgagcga gtactacaag aagggcgacg agatagaagc gatggtgctc aatatcgatt
      661 ccgagaaaca gcggttcagc ctcggtgtca agcagatgag ccacgacccg tggaagatgt
      721 tccgcgaatc gtacaaggag aacgataaaa tagaggccga ggtggtcaag acgaccgatt
      781 tcggcatctt cgtgaaggtg ttcgacacga tcgaagggct cgtccacaag accgagttcg
      841 aagacccgaa caagaacttc tccatcggcg acaagataga ggtcatcatc aagtccatca
      901 gcgacactga acgcaaggtc tccctctcga tcaaggcgat aagcgaggcc gaaagccggc
      961 agaacatgga agaggccaag gcgaagaccg gtgaagtgcc ggaacggctc ggctccatcg
     1021 gcgacgcgtt caagaatcag cagtagtcga cgcctccccc gaaaatttgc tttccccgac
     1081 atcgccccta taatcgcgct gtcttgttca ttacaaccgt gaacttattg aagtaaggag
     1141 tatgccatgc ccgtgaacct gaaaggaaga agttttctga cgctcaagga tttcaccccc
     1201 gaagagatcc gctacctgct cgatctgtcg cgcgacctca aggcgaagaa gcgcgccggt
     1261 atcaaagggg acctgctgga aggcaagaac atcgtcctgc tgttcgaaaa ggcctcgacc
     1321 cgcacccgct gcgccttcga ggtggcggtg ctcgacgagg gcggccatgt caccttcctc
     1381 ggcacgaacg acagccagat gggc
//
LOCUS       DAOQUE010000628         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_130575_length_1401_cov_4.417533, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000628 DAOQUE010000000
VERSION     DAOQUE010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130575_length_1401_cov_4.417533"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PKH10_13670"
     CDS             <1..654
                     /locus_tag="PKH10_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNT29214.1"
                     /translation="VDEQSLRRKDLYGTVSGVTDADGKVLSPQRLLVNFMDNHDLPRF
                     LYDKPDGAALRNALSLLLTMDGIPCLYYGTEQNFDGGNDPANRENLAVSGYDTQNATF
                     RHIAALTALRRELEPLRRGDLTIRWSTDHTGDEEDAGMLAFERTYKGRTALVVLNTHP
                     TRTSKTSFDGNDMQTSFAAGTTLVNRAPGGSAQFTVGTGGILRVSVPPRTLRVLTTN"
     gene            669..1286
                     /locus_tag="PKH10_13675"
     CDS             669..1286
                     /locus_tag="PKH10_13675"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNT29215.1"
                     /translation="MKKHLLTAVLSVLTAALLAAAEPQPASPTEEAPAMETKEAKVLV
                     VYFSRSGNTGAVAKELAAGLGADSEALTEQGTDWSGVWGYFKAGKAAWKEEVSSIEKL
                     KKEPKDYDLVVIGTPIWAWNMTPAVRAFLTVYKDDIKKIAFFATEGGSGHEKAFKNME
                     AIAGKRPVATGFWLEKEVKDPAKLREKISAYVIDIKKTLPPSTEL"
BASE COUNT          298 a          460 c          412 g          231 t
ORIGIN      
        1 gttgacgagc agtctctgcg gcgaaaggac ctttacggca ccgtgtcggg ggtgaccgac
       61 gccgacggaa aggtcctttc gccgcagaga ctgctcgtca acttcatgga caaccacgac
      121 ctgccccggt tcctgtacga caaacccgac ggggcggcgc tccgcaacgc gctgtcgctc
      181 ctcctcacga tggacggcat cccctgcctc tattacggca ccgaacagaa cttcgacggc
      241 ggcaacgacc cggcgaaccg cgagaacctc gccgtgtcgg gctacgacac gcagaacgcc
      301 accttccgtc atatcgcggc gctcacggcg ctccggcgcg aactggagcc gctccggcgc
      361 ggcgatctga cgatacgctg gtcgaccgat cacaccggcg atgaagagga tgccgggatg
      421 ctcgccttcg aacggaccta caagggacgg acggcgctcg tcgtcctgaa cacccacccg
      481 acgcggacct ccaagacctc gttcgacggc aacgacatgc agacctcgtt cgccgccggg
      541 accacactgg tcaaccgcgc ccccggcggt agcgcgcagt tcaccgtcgg cacgggcggg
      601 atcctgcggg tatcggtgcc gccccgaacg ctccgcgtac tgacgaccaa ctaacgggag
      661 ataccaccat gaaaaaacac ctgctgaccg ccgtgctttc ggtcctgacg gccgcccttc
      721 tcgccgccgc cgaaccccag ccggcgtcgc caaccgagga ggcccccgcc atggaaacaa
      781 aggaagcgaa ggtactggtc gtctatttca gccgttcggg caacaccggg gcggtggcga
      841 aggaactggc cgccggactc ggcgccgaca gcgaagcact cactgaacag gggaccgact
      901 ggtcgggcgt ctggggctat ttcaaggcgg gcaaggcggc atggaaagag gaagtgtcgt
      961 ccatcgaaaa actcaagaaa gagccgaagg actacgacct cgtcgtcatc ggcactccca
     1021 tttgggcgtg gaacatgacc ccggcggtcc gcgccttcct gaccgtttac aaggatgaca
     1081 tcaagaagat cgccttcttc gccaccgagg gcggttccgg ccacgaaaag gcgttcaaga
     1141 acatggaggc catagcgggc aaacggcccg tcgccaccgg cttctggctg gagaaagagg
     1201 tgaaggatcc ggcgaaactg cgcgagaaga tatccgccta tgtcatcgat atcaagaaga
     1261 ctcttccccc ctccaccgaa ctctgacctc ttttttgact ttttccccgt tttttgagat
     1321 actccgtcgg tctagggaaa acgcctgttc ccgctgtctt ttataaaccg gaactcatgg
     1381 aggtatgcat gaggatcgtg a
//
LOCUS       DAOQUE010000629         1396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_131386_length_1396_cov_2.702461, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000629 DAOQUE010000000
VERSION     DAOQUE010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131386_length_1396_cov_2.702461"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /gene="murJ"
                     /locus_tag="PKH10_13680"
     CDS             complement(<1..1098)
                     /gene="murJ"
                     /locus_tag="PKH10_13680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNT29216.1"
                     /translation="MNTGAIVRHSLKLAMLTATSRVIGLVREMVRAAYLGTTAFADAF
                     TIAFLLPNLMRRLFAEGSIAVAFVPTFKGYLIENDREKIRTFLSSFLTVLSFLVTVAV
                     LVGIVASDGIISVFYADLTPDIRRETALLTRLMFPYLAFVSIAAFFQGMLNSVNIFGP
                     SGFVPILFNVCVVAATILLSPHLENPARAMTVGVLAGGFLQAAVQLPFALKAGFRFVF
                     RPLRAAFGDPGVRRIFVLIAPTIIGMAAYEVNLLASSVIANTAGTGVVSALQFSNRLL
                     ELLLGVFAVSIGTVVLTELSDNAKRGAWDKFSGHLLFGANAVALVTIPATVFSVLNAT
                     DIVTLVFKARAFDERSVTLTAAIFLFHIAGLY"
     gene            complement(1149..1396)
                     /locus_tag="PKH10_13685"
                     /pseudo
     CDS             complement(1149..1396)
                     /locus_tag="PKH10_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593922.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; incomplete; partial in the middle
                     of a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
BASE COUNT          268 a          393 c          506 g          229 t
ORIGIN      
        1 gtagagtccc gcgatgtgga agaggaatat ggcggcggtg agcgttaccg accgttcgtc
       61 gaaggcgcgg gccttgaaga cgagcgtgac gatgtcggtc gcgttgagca ccgagaagac
      121 ggtcgccggt atcgtgacga gcgccacggc gttggcgccg aagagtaggt gtcccgagaa
      181 tttgtcccac gcgccccgtt tggcgttgtc cgacagttcg gtcagcacga ccgtcccgat
      241 ggataccgcg aagacgccga gcaggagttc gagcaggcgg ttgctgaact ggagcgcgct
      301 caccacgccg gtgccggcgg tgttggcgat gaccgacgag gcgagcaggt tcacctcgta
      361 ggccgccatc ccgatgatgg tgggggcgat gagcacgaat atccggcgta ccccggggtc
      421 gccgaaggcc gcccggagcg ggcggaacac gaaccggaag cccgccttca gcgcgaacgg
      481 caattgcacc gccgcctgca ggaatccgcc cgcgagtacg ccgaccgtca tcgcgcgggc
      541 cgggttttcg aggtggggcg acagcaggat cgtcgcggcg acgacgcaga cattgaacag
      601 tatcgggacg aacccggagg ggccgaagat gttgacgctg ttgagcatcc cctgaaaaaa
      661 ggcggcgatg gagacgaagg cgagataggg gaacatgagc cgcgtgagaa gcgcggtctc
      721 ccgccggatg tcgggggtga ggtcggcgta gaacaccgag atgatgccgt cgctcgccac
      781 gatgccgacg aggaccgcga cggtcacgag gaacgagagc acggtcagga acgacgagag
      841 gaaggtccgt atcttctcgc ggtcgttctc gatgagatag cccttgaagg tcgggacgaa
      901 ggcgaccgcg atgctcccct cggcgaacag ccggcgcata agattaggga gcaggaaggc
      961 gatggtgaag gcgtcggcga aggcggtggt gccgagatag gcggcacgga ccatctcgcg
     1021 gacgagtccg atgacgcggc tcgtcgcggt gagcatcgcg agtttcagac tgtgccggac
     1081 gatcgccccg gtgttcactg caccttcatc tgtgatggat cgacgacatc cttctcttcg
     1141 accgtcgtgg tcggctcggt gccggcgacg gccgtggcga cgatgcgtgc gcggggcttc
     1201 aggttatgct gcttcgccgc ctcttctgat ccgagaagga cgatgcccga tccgtcgacg
     1261 acgcccgatg cgttacccag ggtgtgtaca tgctggatag ccttcagttc cggatagcgt
     1321 cgcagggcga tggcatcgag ttccttttcg ccgatcgtct tgaagatggc gccgagtccc
     1381 gagaggaagg cgtagt
//
LOCUS       DAOQUE010000630         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_132760_length_1387_cov_2.572823, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000630 DAOQUE010000000
VERSION     DAOQUE010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132760_length_1387_cov_2.572823"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PKH10_13690"
     CDS             complement(<1..828)
                     /locus_tag="PKH10_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29217.1"
                     /translation="MSRVRQLIGTGHRFLLLNPPVEDIAAYGLWSAPLGLLRVAAGLR
                     ASEKDLTFFDLLDDPGLHLPPPSIPPEERPDGRHDFWKTVIPKPRELHMVPRRYHRFG
                     ATDDHLRALLADLPRPDAVLIATGMTYWYRTVLRTAELVREHFPAVPVIAGGTAARLI
                     PERLDAGGLIVERGELFPGIDLRGIPDLTERYPAYPIELVRGCPYRCRYCASSIFHAD
                     IGLNDPAAQAEALRAWTTVTGRRDAVFFDDALLWRKREHLRIFLSFVEPGLFRFHTPN
                     "
     gene            complement(825..>1387)
                     /locus_tag="PKH10_13695"
     CDS             complement(825..>1387)
                     /locus_tag="PKH10_13695"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT29218.1"
                     /translation="WLPDLFLGVSLQGKLLGILGYGRIGSAVARRGRAFGMRIAYHAR
                     NRLPREREETETVEYLPLDQIVAQSDVLSLHLPYTPASRHLIDAQAIATMKRTAILIN
                     TARGPLVDETALLAALEDRRLFGAGFDVYEHEPRVDPALLALPNVSVLPHIGSATRET
                     RAAMAAMAIDDCVAVLTGATPRHRVA"
BASE COUNT          245 a          414 c          495 g          233 t
ORIGIN      
        1 gttgggtgtg tggaacctga agaggcccgg ctcgacaaaa gagaggaata tccggaggtg
       61 ttcacgtttc ctccacagca gggcatcgtc gaaaaagacc gcgtcgcgcc tgccggtcac
      121 cgtggtccac gcccgtagcg cctccgcctg tgccgcgggg tcgttcagtc cgatgtcggc
      181 gtggaatatc gaggaggcgc agtaacggca ccggtaggga cagccgcgta ccagttcgat
      241 cgggtaagcg gggtaacgct cggtcaagtc ggggatccca cgcaggtcga tgccggggaa
      301 aagctccccg cgttcgacga tgagcccgcc cgcatcgagc cgttccggga tcagtcgcgc
      361 ggcggtaccg ccggcgatga ccggaacggc ggggaaatgt tcccgcacca gttcggcggt
      421 ccgcaggacg gtgcggtacc agtaggtcat gccggtagcg atgagcaccg cgtctgggcg
      481 cggcaggtcc gcgagcaggg cccggagatg atcgtcggtg gcgccgaacc ggtgatagcg
      541 gcgcggtacc atatgcagtt cccggggttt cggtatcacg gtcttccaga agtcgtgacg
      601 accgtcgggg cgttcttcgg gcggtatcga agggggaggg aggtgcagtc cgggatcgtc
      661 gagcaggtcg aaaaaggtca gatccttttc cgatgcccgg agcccggccg ccacccgcaa
      721 aagtccgagc ggcgcgctcc agaggccgta ggcggcgata tcttcgaccg gcgggttcag
      781 gagaagaaaa cggtgtcccg ttccgatgag ttgacggacc cggctcacgc gacccggtgg
      841 cgcggcgtcg cgccggtgag taccgccacg cagtcgtcta tcgccatcgc ggccatcgct
      901 gcgcgcgttt cgcgagtggc gcttccgata tgggggagta ccgacacatt ggggagggcg
      961 aggagcgcag ggtcgacccg aggctcgtgt tcgtagacat cgaagcccgc gccgaagagg
     1021 cgacgatcct ccagcgccgc gagaagcgcc gtttcatcga cgagtggtcc gcgagccgtg
     1081 tttatcagta tggctgtcct tttcatggtc gcgatcgcct gtgcgtctat gaggtgacgc
     1141 gacgccgggg tgtagggcag gtggagtgaa aggacatcgc tctgtgcgac gatctggtcg
     1201 agcggcagat actcgacggt ctcggtctct tcccgttccc gcggcagacg gttccttgca
     1261 tgataggcga tgcgcatacc gaaggcccgc ccccggcggg cgaccgccga accgatgcga
     1321 ccgtacccga gtatgcccag cagttttccc tgcagactga ccccgaggaa caggtcgggc
     1381 agccagc
//
LOCUS       DAOQUE010000631         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_133819_length_1380_cov_3.934340, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000631 DAOQUE010000000
VERSION     DAOQUE010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133819_length_1380_cov_3.934340"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKH10_13700"
     CDS             complement(<1..476)
                     /locus_tag="PKH10_13700"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT29219.1"
                     /translation="MRVFFCFLAALFALSCGGDEKPKKETFTADTFSEQEVLIGAVTG
                     LMWQSYDPEDVFSYLNRKTYDKAVQYCADLSWAGFNDWRLPTIDELRTIVEGYEDIMW
                     GGRCAVGGDCLQYTCKDKGRESDNDTPCAQRENKYTGPGPDGCYWEAPWRERFCGE"
     gene            complement(510..1301)
                     /locus_tag="PKH10_13705"
     CDS             complement(510..1301)
                     /locus_tag="PKH10_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT29220.1"
                     /translation="MKFEIFFPSTDGSKELIVREEGANWFSALENALKGGGMGHLINN
                     ILCDIKEDGSIHVTETINGKKFLIREFDPNRVAGHEKEIGRTLKKRDPNEVLATVFEE
                     VLDGFDKPEQEGLDFFLDLAMKHIPADSGSIAIAPLAARELTFVACRGPKSDGVKGLK
                     IPMGTGIAGFSALNNVLIAVSDVQKDPRFFKDISEKLGYPTKSIVASPILYNNLTFGM
                     MELINKKESSAFDEDDIHILRFVSEKLGEYLNMIWEARNNNLEDE"
BASE COUNT          251 a          388 c          412 g          329 t
ORIGIN      
        1 tattcgccgc agaaccgttc gcgccacggt gcttcccaat agcagccgtc cggtcccggt
       61 ccggtgtatt tgttctcgcg ctgggcgcac ggggtatcgt tgtcgctctc cctgcccttg
      121 tccttgcagg tgtactggag acagtcgccg ccgaccgcgc accgcccccc ccacatgata
      181 tcctcgtagc cctcgacgat ggtccggagt tcatctatcg tcgggaggcg ccagtcgttg
      241 aatccggccc acgaaaggtc ggcgcagtac tggaccgcct tgtcgtaggt cttgcggtta
      301 aggtacgaga agacgtcctc ggggtcgtaa ctctgccaca taagaccggt caccgcgccg
      361 atgaggacct cctgttccga gaaggtgtcg gcggtaaagg tctctttctt cggtttttca
      421 tcaccgccgc aggaaagcgc gaaaagcgcc gcaaggaaac agaaaaatac tctcatggga
      481 tacctcgcgc cgagaggaaa aaggggtggt tactcgtctt caaggttgtt gttgcgcgct
      541 tcccagatca tgttgaggta ctcccccagt ttctcggaaa cgaaccggag gatgtggatg
      601 tcatcctcgt cgaaggcgga ggattctttc ttgttgatga gctccatcat gccgaaggtg
      661 aggttgttgt acaggatggg ggaggcgacg atggatttgg tcgggtagcc gagtttctcg
      721 ctgatatcct tgaagaaacg ggggtccttc tgcacatcgg agactgcgat gaggacattg
      781 ttgagcgctg agaaaccggc tatgccggtc cccatcggta tcttgagccc cttgacgccg
      841 tccgatttcg gtccgcggca ggcgacgaag gtcagttccc gcgcggcgag tggcgcgata
      901 gcgatcgacc ccgagtcggc cgggatgtgc ttcatcgcga ggtcgaggaa gaaatcgagc
      961 ccctcctgtt ccggcttgtc gaacccgtcg agtacctctt cgaacaccgt ggcgagcact
     1021 tcgttcgggt cgcgcttctt gagtgtgcga ccgatctcct tctcatggcc ggcgacgcgg
     1081 ttcgggtcga attcacggat gaggaatttt ttgccgttta tcgtctcggt gacatggatg
     1141 gacccatcct ccttgatgtc gcagaggatg ttgttgatga gatggcccat gccgccgcct
     1201 ttgagggcgt tctcgagcgc actgaaccag ttggcgccct cctcgcggac gataagttcc
     1261 ttggacccgt cggtcgacgg gaaaaagatc tcgaacttca tgcctcctcc gggatatggt
     1321 ggtccactgt cacaccgaac ggtcgatttt ccgctatttt ttcctattag tcaattcttt
//
LOCUS       DAOQUE010000632         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_133988_length_1379_cov_3.476586, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000632 DAOQUE010000000
VERSION     DAOQUE010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133988_length_1379_cov_3.476586"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1379
                     /locus_tag="PKH10_13710"
     CDS             <1..>1379
                     /locus_tag="PKH10_13710"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNT29221.1"
                     /translation="SIGDFIVVSSDLTSVLSKTRFLIPLAEGEGLHFLHDAYTVFSLS
                     EEREHVPDLVRSRLNIADVSLQPRYSFFMEQEISSSPRNLETVLRYYFLRPEEQPFHS
                     AFEAHEASCREFLYGFLKLGALFDRKQLLAEFHGLVQGTHFSALYDELGGLINPDRTT
                     YEFTSEDAALLEEIAAAAPREFRRLALIDMMLVWKKKRTVLKQRDRLVDLLKEKKRGG
                     GHIFIVASGTSYHAALTAGYFFNDRAGLPVFPANPGMFRSLYLNGLTDKDIIIGVSQS
                     GETKDLVDIFNDVKARYGDAVLLVSLVNNENSTLPQEKSAFYLPILCGPEIAVAATKS
                     FISQIALFHLLADLVRADGAVARDNIEKIVYHLDFTLKNLDPAVTAVANRWFLKPSMH
                     ILGTSLIGLAREGALKVREVVLNHTEGYDAAEFKHGPNTILGDNTIFSLADMERLHGA
                     LLESFDKLA"
BASE COUNT          283 a          468 c          364 g          264 t
ORIGIN      
        1 cagcatcggc gacttcatcg tggtttcctc cgatctcacc tcggtgctct cgaaaacccg
       61 gttcctcatc ccgctcgccg agggtgaagg gctgcatttc ctccacgacg cctacacggt
      121 cttctcgctc tccgaggagc gcgaacatgt gccggacctc gtacgcagcc gactcaacat
      181 cgccgatgtg tcgcttcagc cacgctacag tttcttcatg gaacaggaga tatccagttc
      241 accgcgcaac ctcgaaacgg tcctgcgcta ttatttcctg cggcccgagg aacagccctt
      301 ccactcggca ttcgaggcac acgaagcgtc gtgccgcgag ttcctgtacg gctttctcaa
      361 actcggcgcg ctgttcgacc gcaagcaact gctggcagag ttccacggac tggtgcaggg
      421 cacacatttc agcgccttgt acgatgaact cgggggactt ataaaccccg accgcaccac
      481 ctacgagttc acctccgagg acgcggcgtt gctcgaagag atagcagcgg ccgccccgcg
      541 cgagttccgc agactggctc tcatcgacat gatgctcgtc tggaaaaaga agcgcacggt
      601 cctgaaacaa cgcgatcggc tcgtcgacct cttgaaagaa aagaaacggg gcggaggcca
      661 catcttcatc gtcgcctcgg gcaccagtta tcacgcggcg ctcactgcgg gctatttctt
      721 caacgaccgc gccggcctgc cggtgttccc ggccaacccc ggcatgttcc ggtcgcttta
      781 cctgaacggc cttaccgaca aagacattat catcggcgtc agccagtcgg gcgagaccaa
      841 ggatctcgtg gatatcttca acgatgtgaa ggcgcgctac ggcgacgcgg tgcttctcgt
      901 gtcgctcgtc aacaacgaga actcgaccct gcctcaggag aaatcggcgt tctacctgcc
      961 gatactctgc ggaccggaga tcgcggtggc cgccacaaag tcgttcatca gtcagatcgc
     1021 gctgttccac ctcctcgccg atctcgtccg cgccgacggt gccgtcgccc gggacaacat
     1081 agagaagatc gtctaccatc tcgacttcac gctgaagaac ctcgatccgg ccgtaacggc
     1141 ggtcgccaac cgctggttcc tcaaaccctc gatgcacatc cttggcactt cgctcatcgg
     1201 gctcgcccgc gaaggggcac tcaaggtgcg cgaggttgtc ctgaaccaca ccgaaggata
     1261 cgacgccgcc gagttcaaac atggccccaa cacgatactc ggcgacaaca ccatattctc
     1321 actcgccgac atggaacggc tccacggcgc gctcctcgag tcgttcgaca aactcgccg
//
LOCUS       DAOQUE010000633         1377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_134358_length_1377_cov_3.067322, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000633 DAOQUE010000000
VERSION     DAOQUE010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134358_length_1377_cov_3.067322"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1377
                     /locus_tag="PKH10_13715"
     CDS             <1..>1377
                     /locus_tag="PKH10_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT29222.1"
                     /translation="KNNPVLIGAPGVGKTAIVEGLAQRIVRGDVPTTLERKELFELDL
                     AALVSGAKFRGEFEERLKAVLRAVQESDGRIILFIDELHAIVGAGKAEGSMAAGDMLK
                     PMLARGELHCIGATTLDEYRKHIEKDAALERRFQPVLVSPPTVEDTISILRGLKERFE
                     VHHGITISDGSLIAAATLSDRYVSDRFLPDKAIDLVDEACAMLRTEIDSMPAELDDLR
                     RRILQMEIESTGLKRESDPESKKRRKELDERLAECKGDFEGKKKAWEKEKQEIEALKK
                     IRGEIEEVRRGIERAEREYDLNRAAELKYGKLAKLETELAALVEKQRADDRIISEVLT
                     DREIAEVVSRWTGIPVSKMVESEREKILGLPDRLRERLVGQDEAIATVARSILRSRGG
                     LKDPRRPIGSFIFLGPTGVGKTELARWLAFQLFDSEENMVRVDMSEYMEKHSVSRLIG
                     APPGYVGYD"
BASE COUNT          297 a          423 c          464 g          193 t
ORIGIN      
        1 aagaacaacc cggtcctcat cggcgccccc ggcgtcggca agaccgccat cgtcgagggg
       61 ctcgcccagc ggatagtccg cggcgatgtc ccgacgacgc tcgaacggaa ggaactgttc
      121 gaactcgacc tcgcggcgct cgtatcgggg gcgaaattcc gcggcgaatt cgaggagcgg
      181 ctgaaggcgg tgctgagagc ggtgcaggaa agcgacggtc gcatcatcct cttcatcgac
      241 gaactgcatg cgatagtcgg cgcggggaag gccgagggct cgatggcggc gggcgacatg
      301 ctgaagccca tgctcgcgcg cggcgaactc cactgcatcg gcgcgacgac gctcgacgaa
      361 taccgtaaac atatcgaaaa agacgcggcg ctcgaacggc gcttccagcc ggtgctcgtc
      421 agtccgccga cggtcgagga caccatctcg atactgcgcg gactcaagga gcgcttcgag
      481 gtacaccacg gcatcacgat aagcgacggc tcgctcatcg cggcggcgac cctttctgac
      541 cgctatgtct ccgatcgctt cctccccgac aaggcgatag acctcgtcga cgaggcctgc
      601 gcgatgctgc ggaccgagat agactcgatg ccggccgaac tcgacgacct gcgccgccgc
      661 atcctccaga tggagataga atcgaccggg ctcaagcgcg agagcgaccc cgaaagcaag
      721 aagcggcgga aggaactcga cgagcggctc gccgaatgca agggcgactt cgaggggaaa
      781 aagaaggcgt gggaaaagga aaagcaggag atcgaggcgc tcaagaagat acgcggcgag
      841 atagaggagg tgcggcgcgg catcgaacgg gccgagcgcg aatacgacct caaccgcgcg
      901 gcggaactga aatacgggaa actcgccaaa ctcgagaccg aactggcggc actggtcgag
      961 aaacagcgcg ccgacgaccg catcatctcc gaggtactga ccgatcgcga gatcgccgag
     1021 gtggtgagcc gctggaccgg catccccgtc tcgaagatgg tcgaaagcga gcgcgagaag
     1081 atactggggc tcccggatcg gttgcgtgaa cggctcgtcg gacaggacga ggcgatagcg
     1141 acggtggcgc gctcgatact gcggtcgcgc ggcgggctca aggatccgcg ccgtcccatc
     1201 ggcagtttca tcttcctcgg gcccaccggc gtcggcaaga ccgaactcgc gcggtggctc
     1261 gcgttccagc tgttcgactc cgaggagaac atggtccgcg tcgacatgtc cgagtacatg
     1321 gagaagcatt cggtgtcgcg gctcatcggg gcgcctcccg gctatgtcgg ctacgac
//
LOCUS       DAOQUE010000634         1375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_134675_length_1375_cov_3.540909, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000634 DAOQUE010000000
VERSION     DAOQUE010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134675_length_1375_cov_3.540909"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..>1375)
                     /locus_tag="PKH10_13720"
     CDS             complement(272..>1375)
                     /locus_tag="PKH10_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNT29223.1"
                     /translation="GQLPQTDALKRGVDVLIATPGRLLDLIGQGYIKLDTLSIFVLDE
                     ADRMLDMGFIHDIRKIIDKLPKKRQTLLFSATMPPDIVSLASAYLTSPVRIEVTPQAT
                     PVEVIEQSVYFVEKNDKRHLLAHILKDESVVSALVFTRTKHGADRVVKDLQRAGINAQ
                     AIHGNKSQNARQRALGDFKSGHTRVLVATDIAARGIDIDELSHVINFDIPEVPETYVH
                     RIGRTGRAGLSGIALTFCDADERAYLRDIQKLIGRALPAVEEHPFRSTVPLTNMEPRR
                     EERRPERRPEKRNEREPRRERQQRPRHEPKKEPKPESGAVSDSAVTRPDPKPAPEPER
                     RRDDRRPRPHGPRPKDDRPFGRQKPKGGLPRVG"
BASE COUNT          204 a          409 c          464 g          298 t
ORIGIN      
        1 cgtcggtcgt ctcgatgtcg ccggtgcctg tcgggtcgtc ggggacgctc ccgtcagtgg
       61 gcgactgttt ggtgttctcg caggagccga gaagcagtat cgcgaacaac cccgcgataa
      121 cataaatgaa actcttaaaa ttcatggtaa cctccattca aaaaggacgc cggtttggta
      181 cccgggcgcc acccttttta tcattttcgg gcacattttg caagaactat ttgtttttac
      241 aagatctttc gtcgtcaccg caccagaccg gctatccgac gcgcggcagg ccgcctttcg
      301 gtttctgtct tccgaagggg cggtcgtcct tggggcgcgg gccgtgcgga cgcgggcgcc
      361 gatcgtcgcg gcgccgctcc ggttcggggg ccggtttcgg atccggcctt gtcacggcgg
      421 agtcggagac ggcgccggat tcgggcttcg gttccttctt cggttcgtgg cgcggtcgct
      481 gttgccgttc acgacgcggc tcgcgttcgt tccttttttc gggacgacgc tccggtctcc
      541 gctcctcgcg gcgcggctcc atgttggtga gcggcacggt cgagcggaaa ggatgttcct
      601 cgaccgccgg caaggcgcgg ccgatgagtt tctgtatgtc gcgcagatac gcccgttcat
      661 cggcgtcgca gaaggtcagt gcgatgcccg acagccccgc gcgaccggtg cggccgatgc
      721 ggtggacata ggtctcaggc acctcgggga tatcgaagtt gatgacatgc gacagttcgt
      781 cgatgtcgat gccgcgcgcc gcgatgtcgg tcgcgacgag cacccgggtg tggccgctct
      841 tgaaatcgcc gagcgcccgc tgacgggcgt tctgcgactt gttcccgtgg atcgcctgcg
      901 cgttgatgcc ggcccgttgg aggtccttga cgacgcggtc ggcaccgtgc ttggtccgcg
      961 tgaagacgag cgccgacacg accgattcgt ccttcaggat gtgggcgagc aggtgccgtt
     1021 tgtcgttctt ctcgacgaag tagaccgact gctcgatcac ctcgaccggc gtcgcctgcg
     1081 gcgtcacctc gatgcgcacc gggcttgtca ggtaggccga cgcgagcgac acgatatcgg
     1141 gcggcatggt tgccgagaac agcagcgtct gccgtttctt gggcagtttg tcgatgatct
     1201 tgcgaatgtc gtggatgaag cccatgtcga gcatccggtc ggcctcgtcg agcacgaaga
     1261 tgctcagggt gtcgagtttg atatagccct gcccgatgag gtcgagcagg cggcccggcg
     1321 tcgcgatgag gacatcgacg ccgcgcttga gggcgtcggt ctgtggcagt tggcc
//
LOCUS       DAOQUE010000635         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_134817_length_1374_cov_4.474602, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000635 DAOQUE010000000
VERSION     DAOQUE010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134817_length_1374_cov_4.474602"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /gene="thiC"
                     /locus_tag="PKH10_13725"
     CDS             complement(<1..1210)
                     /gene="thiC"
                     /locus_tag="PKH10_13725"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNT29224.1"
                     /translation="MTQLEAARAGKITEEMRHAAQVERMDAEEMRRRIAAGRVVICRN
                     TVHDIAPLAIGEGCRVKVNANIGTSEDVNSLEEELEKARIAVRYGADAVMDLSTGGDL
                     ELTRKKIMAAAPVAIGTVPIYQAIVQVVRELGKSFAEMTPDEIFGAIDRQVGSGVDFI
                     TVHCGVNRHLLDGLKDRPRLMGIVSRGGSITARWMAHNNAENPLYDRFDDLLEIAHAH
                     DCVLSLGDGLRPGALADATDHAQIEELITLGQLTERARAKGVQVMIEGPGHVPLQQVA
                     ANMQMQKALCSGAPFYVLGPLVTDVAPGYDHITSAIGGALAAMSGADFLCYVTPAEHL
                     SLPTVDDVKEGVIASRIAAHAADIAKGWPGAADWDREMSCARRKLDWKRQEELALDPE
                     KVRRYRDRRGP"
BASE COUNT          223 a          460 c          427 g          264 t
ORIGIN      
        1 tcgggccgcg ccgatcgcgg taacggcgca ccttctcggg gtcgagcgcc agttcctcct
       61 gccgtttcca gtcgagtttg cggcgcgcac aggacatctc gcggtcccag tcggcggcgc
      121 cgggccaccc cttggcgatg tcggcggcgt gggccgcgat gcgcgacgcg atgacgccct
      181 ccttcacatc gtcgaccgtc gggagcgaca ggtgttcggc cggcgtcaca tagcacagga
      241 aatcggcgcc gctcatcgcc gcgagcgccc cgccgatggc cgaggtgata tggtcatagc
      301 cgggcgcgac atcggtgacg agcgggccaa gcacatagaa cggcgccccg ctgcagagcg
      361 ccttctgcat ctgcatattg gcggcgacct gctggagcgg cacatggccc ggtccctcga
      421 tcatcacctg cactcccttc gcgcgggccc gttcggtgag ttggccgagc gttatcaact
      481 cttctatctg ggcgtgatcg gtggcgtcgg cgagcgcgcc gggccgcagt ccgtcgccga
      541 gcgacaggac acaatcgtgg gcgtgtgcta tctcgagcag atcgtcaaat cgatcgtaca
      601 gcgggttctc ggcgttgttg tgcgccatcc agcgggccgt tatcgagccg ccgcgcgaaa
      661 cgatgcccat gagccgcggt cgatccttca gcccgtcaag caggtgacgg ttgacgccgc
      721 agtggaccgt gatgaagtcg accccgcttc ccacctgccg atcgatcgcg ccgaatatct
      781 cgtcgggggt catctcggcg aacgacttgc cgagttcgcg gaccacctgc acgatcgcct
      841 gatagatcgg gacggtgccg atggcgaccg gggccgccgc catgatcttt ttgcgggtga
      901 gttcaagatc gccgccggtc gagaggtcca tcaccgcgtc ggcgccgtat ctgacggcga
      961 tacgcgcctt ttcgagttcc tcttcgagcg agttgacatc ctcgctggtg ccgatgttgg
     1021 cgttcacctt gacgcggcat ccttcgccga tcgcgagcgg cgcaatgtca tggacggtgt
     1081 tgcggcatat cacgacgcgc cccgcggcga tgcggcgccg catctcctcg gcatccatcc
     1141 gctcgacctg cgcggcgtgc ctcatctctt cggtgatctt cccggcgcgg gccgcttcca
     1201 actgggtcat tttcgtcata tctcaacctc atctaacaaa cgcgaaaggt actgtacatc
     1261 accttccgcc gtttgaccat aaaaaagaga tatcgccgca aggtctcgaa aacctttccg
     1321 tatcaatcct tcggcacgga cgcggtcgat gccgccgagc aggaacaccc cctg
//
LOCUS       DAOQUE010000636         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_135000_length_1373_cov_3.575873, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000636 DAOQUE010000000
VERSION     DAOQUE010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135000_length_1373_cov_3.575873"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..830)
                     /locus_tag="PKH10_13730"
     CDS             complement(48..830)
                     /locus_tag="PKH10_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT29225.1"
                     /translation="MRVLLTAQGLNVSIGSAAILHDISLELPEHGILSVIGPNGAGKS
                     TLLKCLAGLLAPTAGRVELAGKEIGTLSGRDRAKMIAVVPQEANFTVPFTVAAFIELA
                     RYPHRGSFSPLTAADRTAIGQAVAVTGIAPLLPRTVTTLSGGEKRLVLIAAALAQESP
                     LLLLDEPTAALDPAHRAALLRLLKKAHGELGRTIVLVTHEVNDALFLGGNVLGLKKGR
                     VSLPMIAVDALALPALGALFDIPFAHWQDPAGRRLVAPEVSS"
     gene            complement(827..>1373)
                     /locus_tag="PKH10_13735"
     CDS             complement(827..>1373)
                     /locus_tag="PKH10_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT29226.1"
                     /translation="IATVALPAKELGDVAASARIAGSACGVEKEADAFVIRFDTALKQ
                     AAERSAGKQRPKVLVAVGRDHDATGIKGLFVAGKGSFHDELIAKAGGENACTVGIPYP
                     RISAEGLIAMNPDIIIEFVSDDEAQTASAADWKGFDTVGAVKNGRVHIVTGDRSFIPG
                     PRAPLLLEELARLIHPDTVTS"
BASE COUNT          232 a          445 c          461 g          235 t
ORIGIN      
        1 tatcgccacc gccgcgacgg cgatgagaat aaggctcaac gaccgtttca tgacgacacc
       61 tccggcgcga ccagacgccg ccccgcgggg tcttgccagt gggcgaacgg gatatcgaac
      121 agcgccccga gcgccggaag agcgagcgcg tccacggcga tcatcggaag gctgacacgc
      181 ccctttttaa gtcccagcac attcccgccg aggaagaggg catcattcac ctcatgggtc
      241 accagcacga tggtgcggcc gagttcgccg tgcgccttct tgagcaagcg cagtagcgcg
      301 gcgcggtggg ccggatcgag cgcggcggtc ggctcgtcga ggagtaggag cgggctttcc
      361 tgtgcgagcg ccgccgcgat gaggacgagc cgtttctcgc cccccgaaag ggtcgtcacg
      421 gtgcggggaa ggagcggcgc gataccggtc accgcgaccg cctgtccgat cgcggtacgg
      481 tcggcggcgg tgagcggcga aaaggagccg cgatggggat agcgcgccag ttcgatgaac
      541 gccgcgacgg tgaacgggac ggtgaagttc gcctcctgcg gcacgacggc gatcatcttc
      601 gcccggtcgc ggcccgaaag cgttcctatc tcttttcccg ccagttccac gcggcccgcg
      661 gtcggcgcga ggagtcccgc gagacacttg agcaaggtcg atttccccgc gccgttcggc
      721 ccgatgaccg acagaatgcc gtgttccggc aactccagcg agatgtcgtg cagtatcgcc
      781 gcagatccga tggaaacatt cagtccctgt gcagtcagaa gcaccctcat gatgttaccg
      841 tatcaggatg tatcagccgg gcgagttctt cgaggaggag cggggcgcgc gggccgggga
      901 tgaaggagcg gtcgccggtg acgatgtgga cgcggccgtt tttcaccgcg ccgacggtgt
      961 cgaaaccttt ccagtcggcg gccgaggcgg tctgcgcttc gtcatcggag acgaattcga
     1021 tgatgatgtc ggggttcatc gcgatgagtc cttcggccga tatgcgcgga tagggaatgc
     1081 ccaccgtgca ggcgttctcg ccacccgcct tggcgatgag ttcgtcgtgg aaactcccct
     1141 tccccgcgac gaaaagcccc ttgatgccgg tcgcgtcatg gtcgcgcccc accgccacga
     1201 gcaccttcgg ccgctgcttt ccggcgctcc gttcggccgc ctgtttgagc gccgtgtcaa
     1261 aacggatgac gaacgcgtca gcctcttttt ctacgccgca ggcgctcccc gcgatccgcg
     1321 ccgaggcggc gacatcgccc agttcctttg ccgggagcgc gaccgtcgcg atg
//
LOCUS       DAOQUE010000637         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_135146_length_1372_cov_4.132118, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000637 DAOQUE010000000
VERSION     DAOQUE010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135146_length_1372_cov_4.132118"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1372
                     /locus_tag="PKH10_13740"
     CDS             <1..>1372
                     /locus_tag="PKH10_13740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT29227.1"
                     /translation="GAPRNLVFADIRWSALDETTLVGRIARYALYFSIATVLALLALL
                     SIPFVPRSSGVGIYSMALIYAIAAIPFYFSGVVVSAILSTRFIALVSGLYAADLAGAA
                     AGALLFFFFLRLTDAISFVILVAALPAVAAYLLARHRAALALCLLFVLVAGANHFERF
                     FRIEWQKVETGQLIVAVDNLTEWEGWTPFSRITVQNYIDSAFGWGTSNAFPEEFPDYK
                     VGQKNLIIDAAAATIITMRGKPIEELQHLRYDITNLAHVVVPDARVAVIGVGGGRDIL
                     SALHFNQKEIWGIEINERILEAITGPYREFTYPFADFPNVHLVHDEARSFIERTDLTF
                     DIIQASLIDSWAATASGAFVLTENSLYTVEGWEAFLSKLTDRGVVTMSRWYYKKRPGE
                     ILRLASLAYEALAAAGVEKPAAHIMLAATTYAGEEPPGLTAEERRAREDVAYGTGTII
                     VSKKPFD"
BASE COUNT          231 a          478 c          395 g          268 t
ORIGIN      
        1 ggcgcgcccc gcaacctggt gttcgccgac atccgctggt cggcgctcga cgagacaacg
       61 ctggtcggcc gtatcgcccg ttatgcgctc tacttctcga tcgcgacggt gcttgcgctc
      121 ctcgcgctcc tctcgatacc attcgtccct cgttccagcg gcgtcggcat ctattcgatg
      181 gccctcatct acgccatcgc ggcgatcccc ttctacttct cgggcgtcgt cgtgtcggcg
      241 atactgtcga cccgtttcat cgcgctcgtg agcggcctgt atgccgccga cctcgccggc
      301 gcggcggccg gggcgctgct cttcttcttt ttcctgcggt tgaccgacgc gatcagtttt
      361 gtcatactcg tcgcggcgct cccggcggtc gccgcctatc tgctcgcccg ccaccgcgcg
      421 gcgctcgcgc tctgcctcct tttcgtcctc gtcgccggag ccaaccactt cgaacggttc
      481 ttccgcatcg agtggcagaa ggtcgagacg ggtcaactca tcgtcgcggt cgacaacctc
      541 accgaatggg agggatggac cccattctcg cggataaccg tccagaatta catcgattcc
      601 gccttcggct ggggcacatc gaacgccttt cccgaggagt tccccgacta taaggtgggg
      661 cagaagaacc tcatcatcga cgcggccgcg gcgaccatca tcacgatgcg cgggaaaccg
      721 atagaggaac tccagcatct ccggtacgac atcaccaacc tcgcccatgt cgtcgttccc
      781 gacgcgcggg tggcggtcat cggcgtcggc ggcgggcgcg atatcctctc cgcgctccac
      841 ttcaaccaga aggagatctg gggcatcgag ataaacgaac ggatactcga ggcgataacc
      901 ggcccgtacc gtgaattcac ctatccgttc gccgacttcc cgaatgtaca tctcgtccac
      961 gacgaggcgc gcagtttcat cgaacggacc gatcttacct tcgacatcat acaggcgtcg
     1021 ctcatcgact cgtgggcggc gaccgcgtcg ggcgccttcg tcctgaccga gaattcgctt
     1081 tacaccgtcg agggatggga ggcgttcctg tcgaaactga ccgaccgtgg cgtcgtaacg
     1141 atgtcgcgct ggtactacaa gaaacgaccc ggcgagatat tgcggctcgc ctcgcttgcc
     1201 tacgaggcgc ttgccgccgc gggggtcgag aaacccgccg cccacatcat gctcgccgcg
     1261 accacctatg cgggcgagga gccgcccggc ctcaccgcgg aagagcgacg ggcccgcgag
     1321 gatgtcgcct acggcaccgg caccatcatc gtttcgaaaa aaccgttcga cg
//
LOCUS       DAOQUE010000638         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_136507_length_1364_cov_3.293354, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000638 DAOQUE010000000
VERSION     DAOQUE010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136507_length_1364_cov_3.293354"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..330)
                     /gene="yajC"
                     /locus_tag="PKH10_13745"
     CDS             complement(34..330)
                     /gene="yajC"
                     /locus_tag="PKH10_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNT29228.1"
                     /translation="MGAIFNMLVPFVLIFVIFYFLVVMPQKKQQKKLQEMLGSLQKGD
                     RVITSSGIFGRVTEVRNDSFKIEIAPKVVVSVQKSAVISRVAEDVDPGEGTETK"
     gene            697..>1364
                     /locus_tag="PKH10_13750"
     CDS             697..>1364
                     /locus_tag="PKH10_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29229.1"
                     /translation="MKTNVMLLVLSMVLLSLVSCGGDDPTIPTSDNAPVGDSDTTVTP
                     DTSLPDNQLPDSDTTVTDNAGSDSDTPVADNEIPDTDTNTGDCATANLGKNCKIDGDC
                     GACLICVNAKCAEGCQGDGDCASYAGTTCNKKLARCVNTVGSSGACNETNCPAGCCYA
                     DKGFKTLKCLASAALQTCGICPQGQVYMDGKQCIPSACKVEDGKCKTYNSTEVRADCY
                     ECKTG"
BASE COUNT          324 a          367 c          373 g          300 t
ORIGIN      
        1 ccatcaagag ttcatgacct gtttcccgac ctttcatttc gtttcggtcc cctcgccggg
       61 gtcgacatcc tcggcgaccc gggatatcac ggcgcttttc tgcaccgaga ccaccacttt
      121 cggggctatc tcgatcttga aagagtcgtt gcggacctcg gtcacccgac cgaagatacc
      181 cgaggaggtg atcacgcggt cccccttctg gagagacccc agcatctcct gcaatttttt
      241 ctgttgtttt ttttgcggca tgacgacgag aaaatagaaa atgacgaaaa tgaggacgaa
      301 ggggacgagc atgttgaata tggcgcccac gccatcagcc gggggggcgc tttgaacaac
      361 aagaagatca gataatcttg aaatcattga ggtattcacc atttcctcca agcaaaaaac
      421 aaaaaagcga ggcatggtaa ctccgaaacg caaataaggc aagattttat tttcctgcgt
      481 ggatgggtca taattttttg cgcgagagcg ggaaactttt tcgcctcccg gggggtctac
      541 actttcggag cactagagat caaggtgaga aatggtcagg gtcgatcaga agcacggtga
      601 aaaaaatagt tgctattcat cttttacctg ttatagtgag ccatatttta tggtgtgttc
      661 cttgtgatat attttgcttt tcaaggaggc gacgcgatga agacgaatgt tatgttgctc
      721 gttctgagca tggtgctctt gtcccttgtt tcctgcggcg gtgatgaccc gaccatcccg
      781 acgagtgaca atgctccggt cggcgattcc gacaccacgg tgacgccgga cacctccctg
      841 ccggacaatc agttgcccga cagtgatacc acggtgaccg acaacgccgg cagtgacagt
      901 gacacgcccg tagcggacaa cgagatcccc gacaccgata ccaacaccgg tgactgcgct
      961 accgccaatc tcggcaagaa ctgcaagata gacggtgact gcggcgcctg cctcatctgc
     1021 gtcaacgcga agtgcgccga aggctgtcag ggcgacgggg actgcgcttc ttatgccggc
     1081 accacctgca acaagaaact tgcccgttgc gtgaacactg tcggctcgag cggcgcatgc
     1141 aacgagacca actgcccggc cggctgctgc tacgccgaca aaggtttcaa gaccttgaag
     1201 tgtcttgcct cggcggccct gcagacctgt ggtatctgcc cgcagggtca ggtgtatatg
     1261 gatggcaaac agtgcatccc gtcggcgtgc aaagtggaag acggcaagtg caagacctac
     1321 aattcgaccg aggtgcgcgc cgattgttac gaatgtaaga ccgg
//
LOCUS       DAOQUE010000639         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_137081_length_1360_cov_6.732567, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000639 DAOQUE010000000
VERSION     DAOQUE010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137081_length_1360_cov_6.732567"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PKH10_13755"
     CDS             <1..768
                     /locus_tag="PKH10_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29230.1"
                     /translation="TTLGGWEDPALCDWDCDTDFAEEGGACIDQKDVPCNIDNANPVN
                     SHDVIADVTIHYTTLGGWEDPALCDWDCDTGYELNGSDCVPAKIKFADWPLATDGTPS
                     NVATNVTAGNFSYLGVTLVAGGLNYSETSAWSNAATPDTAKYFQITIAPQTGYALQVT
                     DLTFTHQRTNAGGPANYQVFYSLDGFATSTQLAPATATTTTATPGDLTGLSINVASGQ
                     TLTVRWYGYDATAAGGGLRRWAFISNTTSVSGYITQP"
     gene            complement(829..>1360)
                     /locus_tag="PKH10_13760"
     CDS             complement(829..>1360)
                     /locus_tag="PKH10_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HNT29231.1"
                     /translation="AAILFPETHFDRVRVGIAQYGLWPSKETRLSYLTRHGAGAEHLL
                     RPVLCWKTRIVQIKDIPSGSFIGYGCTYQTTRASRIAILPIGYSDGYDRQLSNSAHVL
                     VKGQRAPVRGRVAMNLTAIDITDIPGVELEEVVVLLGRQGEQEITADRLAEMAGTINY
                     EIVARLAPHIPRIVVP"
BASE COUNT          252 a          434 c          407 g          267 t
ORIGIN      
        1 accacgctcg gcggctggga agacccggcg ctctgcgatt gggactgcga caccgacttc
       61 gccgaggaag gcggcgcctg catcgatcag aaggatgtgc cgtgtaacat cgataacgcc
      121 aacccggtga acagtcatga cgtcatcgcg gatgtcacca ttcactacac cacgctcggc
      181 ggctgggaag acccggcgct ctgcgattgg gactgcgaca ccggttacga attgaacggt
      241 tcggactgtg tgccggccaa gatcaagttc gccgactggc cgctggcgac cgacggaaca
      301 ccctccaatg tggccacgaa tgtcaccgcc ggcaatttct catacctcgg tgtaacttta
      361 gtagcgggtg gcctcaatta ttcggaaacg agtgcgtggt ctaacgcggc tacccccgat
      421 accgccaaat attttcagat aacgatcgct ccccagaccg gctatgcgtt acaggtgacc
      481 gaccttacct tcactcatca gcgcaccaat gccggcggac cggcgaacta tcaggtgttc
      541 tattctctgg acggtttcgc cacctctacg cagttagcac ctgccaccgc cacgacgacg
      601 accgccactc ccggcgatct taccgggctg tcgatcaatg ttgcctccgg gcagaccctg
      661 actgtcagat ggtatggcta tgacgccact gccgccggcg gcggccttcg ccgttgggct
      721 ttcatcagca acacgaccag cgtcagcggc tacataactc agccgtaact ctttcttctt
      781 tctccgacac ttcgcggggg gctttcgggc cccccgttct tttttgactt acgggacgac
      841 gatgcgtggg atatgcggcg cgaggcgcgc cacgatctcg tagttgatgg tgcccgccat
      901 ctcggcaaga cgatcggccg ttatctcctg ttccccctga cgaccgagca ggaccaccac
      961 ctcctcgagt tcgacgccgg gaatgtcggt gatgtctatc gcggtgaggt tcatcgcgac
     1021 gcggccgcgg accggggccc gctgtccctt caccagcaca tgggcgctgt tggaaagttg
     1081 ccggtcgtag ccgtcggagt agccgatggg cagtatcgcg atgcgactcg cccgggtcgt
     1141 ctgataggtg cacccgtacc cgatgaacga accggagggg atatccttta tctgcacgat
     1201 gcgcgtcttc cagcacagga cggggcgcag gagatgctcg gcgccggcgc cgtggcgggt
     1261 gaggtacgac aggcgggtct ccttggatgg ccacagcccg tactgcgcga tgccgacccg
     1321 cacccggtcg aaatgggtct cggggaacag tatcgccgcg
//
LOCUS       DAOQUE010000640         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_137265_length_1359_cov_3.774540, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000640 DAOQUE010000000
VERSION     DAOQUE010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137265_length_1359_cov_3.774540"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PKH10_13765"
     CDS             <1..813
                     /locus_tag="PKH10_13765"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HNT29232.1"
                     /translation="SAAGTGVVAQNPGDSVAYTIDQYQILTLNSAPGGNYGADLSGTF
                     ISADKKIAVFGGHRCTNVPADTPACDHLEHQMFPLRSWGKDYVVVRTRPRGSENDNIR
                     FLASEDNTTINWTGGLSGTVVLNAGQRHDIWTTTDFTVSADKPIMVGQILASQDAGAG
                     TGDPAMMLIAPNEQMRNDYIFLVAPNYDYDRLTIVSQANNQITLDGTVMDSNTFVPIP
                     GTPWLRQYVDTNDGAHTLTSTSPVALYVYGFSQYVSYAYTAGLDLLKINDQD"
     gene            813..1220
                     /locus_tag="PKH10_13770"
     CDS             813..1220
                     /locus_tag="PKH10_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29233.1"
                     /translation="MMLDLRERILLAMKRYGVLSFGQLHELFPVQSVTHLAEVLHSMV
                     ADGLMSKHIDTDASTTFYYRRFYTASGDHIIFHFRDHPHPERFAAEPFLNEMPWFFEP
                     IEYDDTAVYSSNFPDPTGALAAAEETEKEESPE"
BASE COUNT          283 a          457 c          380 g          239 t
ORIGIN      
        1 tcggccgccg gcaccggcgt ggtcgcgcag aacccgggcg actcggtggc ctacacgatc
       61 gaccaatacc agatactcac gctcaactcc gctcccggcg gcaactacgg cgccgacctt
      121 tcgggcacct ttatctcggc cgacaagaag atagcggtgt tcggcggcca ccggtgcacc
      181 aatgtgccgg ccgacacgcc ggcctgcgac catcttgagc accagatgtt cccgctccgt
      241 tcatggggca aggactatgt cgtggtccgt acgagacctc gcggaagcga gaacgacaac
      301 atccgtttcc tcgcctccga ggacaacacg acgatcaact ggaccggcgg cctgtcgggg
      361 acggtggtgc tcaacgccgg acagcgacat gacatctgga ccaccaccga cttcaccgta
      421 tccgccgaca agccgatcat ggtcggccag atactcgcct cacaggacgc gggagccggc
      481 accggcgacc cggcgatgat gctgatagcc ccgaacgagc agatgcgcaa cgactacatc
      541 ttcctcgtcg cgccgaatta cgattacgac cggctcacca tcgtgtcgca ggcgaacaat
      601 cagataacgc tcgacggcac cgtcatggat tcgaacacct tcgtgccgat accgggcacc
      661 ccgtggctcc gccagtatgt cgacacgaac gacggagccc ataccctgac ctcgaccagc
      721 ccggtcgcgc tgtatgtgta cgggttctca cagtatgttt cctatgccta caccgcgggg
      781 ctcgatcttc tgaagataaa cgatcaggac tgatgatgct cgacctgcgc gaacggatac
      841 tgctggccat gaaacgatac ggcgtcctct ccttcggcca gttgcacgaa ctgttcccgg
      901 tgcagtcggt gacccacctc gccgaggtgc ttcactcgat ggtcgccgac ggtctgatga
      961 gcaaacatat cgacaccgat gccagcacca ccttctacta ccgccgcttc tacaccgcgt
     1021 cgggcgacca catcatcttt catttccgcg atcatccgca cccggaacga ttcgcggccg
     1081 aaccgttcct caacgagatg ccgtggttct tcgagccgat agagtacgac gatacggcgg
     1141 tctattcctc gaatttcccc gacccgacgg gcgcgcttgc ggcggccgaa gaaactgaaa
     1201 aggaagaatc cccggaataa cccgcccctc cccctccctc gaaacgaggg cggggcttct
     1261 gacggcaatg caattatgcg gcgtgcttgg tcttgaagcc cttctgcatc cagttgcggg
     1321 cgagcaggag cccgaggggg gacacgaggg cgatgaagc
//
LOCUS       DAOQUE010000641         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_139411_length_1346_cov_3.738187, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000641 DAOQUE010000000
VERSION     DAOQUE010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139411_length_1346_cov_3.738187"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PKH10_13775"
     CDS             <1..928
                     /locus_tag="PKH10_13775"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HNT29234.1"
                     /translation="PLALAEVSKEQNFTKPPSRYTEGSLVKELEEKGIGRPSTYAAII
                     NHIIARKYVDRLKEPKGTLGSTDLGGLVSDTLQQFFPKIINVAFTAEMEEKLDRIEAG
                     EAKWLAVLKEFYAAFEENRAESLETLKESGKKRIYAEENCPKCGGRLVVRFSKKGNIP
                     FLSCEKYPDCTFSGDMVKDGDKIHLGENRRDEPQLLEEKCPNCGAALAMKQGRFGPFK
                     ACSNYPKCKTIVREIEEFGPCPKKDCGGTVQKRRSQKGKVFYGCSHYPKCDFVSWYPP
                     IAGQTCAKCGAAYMVEKKSGIACGICGEKVKS"
     gene            929..>1346
                     /locus_tag="PKH10_13780"
     CDS             929..>1346
                     /locus_tag="PKH10_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT29235.1"
                     /translation="MKRVTILGAGLAGCEAALQLAARGIAVTLVEAKPEKVEPAIYRE
                     DGPAELVCSNSLKSESNATPSYQLKAECRIAGSFLLQAADAVKVAAGESLAVDRERFS
                     RAVAALIEGEPLITFVRGTVLDSLDALRERFPADLYI"
BASE COUNT          300 a          426 c          407 g          213 t
ORIGIN      
        1 gccgctcgcg ctcgccgaag tatccaagga acagaatttc accaaaccgc cgtcccgcta
       61 cacggaagga tcgctggtca aggaactcga agagaaaggt atcggccgcc cctcgaccta
      121 cgccgccatc ataaaccaca tcatcgcccg caagtatgtc gaccggctca aggaacccaa
      181 ggggacgctc gggtcgaccg atctgggcgg tctggtgagc gacacgctcc aacagttctt
      241 cccgaagatc atcaatgtgg cgttcaccgc cgagatggag gagaaactcg accgtatcga
      301 ggcaggcgag gcgaaatggc tcgcggtgct caaggaattc tacgccgcct tcgaggagaa
      361 ccgcgccgaa agtctcgaaa cgctcaagga gtcgggcaag aaacggatat acgccgaaga
      421 gaactgcccc aagtgcggcg gccggctcgt cgtccggttc tccaagaagg gcaacatccc
      481 cttcctttcc tgtgaaaaat atcccgactg caccttctcg ggcgacatgg tcaaggatgg
      541 cgacaagatc catctcggcg agaaccgtcg cgatgagccc caactgctcg aggagaaatg
      601 tcccaactgc ggcgcggcgc tcgccatgaa acagggaagg ttcggtccct tcaaggcctg
      661 ttcgaactac ccgaaatgca agaccatcgt ccgcgagatc gaggagttcg gcccctgccc
      721 gaagaaggac tgcggcggca ccgtgcagaa acggcgctcc cagaaaggca aggtgttcta
      781 cggctgcagc cattacccga aatgcgactt tgtctcgtgg tatcccccga tagcgggcca
      841 gacctgcgcc aaatgcggcg ccgcctacat ggtggagaag aaatcgggca tcgcctgcgg
      901 catctgcggc gagaaggtca agtcctaggt gaaacgcgtc accatcctcg gcgccgggct
      961 cgccggctgt gaggcggcgc tccaactcgc ggcgcggggc atagccgtca cgctcgtcga
     1021 agcaaaaccg gaaaaggttg agccggcgat ctaccgggag gacggccccg ccgaactggt
     1081 ctgcagcaac tcgctcaaat cggaatcgaa cgcgacgccg agttaccaac tgaaggcgga
     1141 atgccgcatc gccggatcgt tccttctgca ggcggccgac gcggtgaagg tggcggcggg
     1201 cgaaagtctc gcggtcgacc gcgaacggtt ctcgcgggcg gtcgcggcgc tcatcgaggg
     1261 agaaccgctc atcaccttcg ttcgcggcac cgtcctcgac tcgctcgatg ctctgcgcga
     1321 acgattcccc gccgaccttt acatca
//
LOCUS       DAOQUE010000642         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_143525_length_1323_cov_2.703470, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000642 DAOQUE010000000
VERSION     DAOQUE010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143525_length_1323_cov_2.703470"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="PKH10_13785"
     CDS             complement(<1..933)
                     /locus_tag="PKH10_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29236.1"
                     /translation="MKEYYRSFTALSSFKNPDRAALLPYIHRQKTEISALHTIRYDFW
                     TTLDYTIDLTMRLLKNPKETVGEPTQTVLRPLFGTINRELNTILQKVTDDEFGYLKHE
                     LKVFHHIVTHTINLVDKNVAHFVPRFLFVSLIEDMRLLYPLCERTLNPALLARRDYRL
                     LITRHLAGRFDKRLVGIMDLLLWYREVAPVMVREDRQEYHGKLMALVRAALDQPVEFL
                     GSDAYSLRDMIVELRETVTGSLIAERFMAILNELSYVPADMQLDADKWLNDLCAVFIT
                     SPIEERKNNAVLRDLSIAGEEKVPVQSVGILRLGR"
     gene            complement(930..>1323)
                     /locus_tag="PKH10_13790"
     CDS             complement(930..>1323)
                     /locus_tag="PKH10_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HNT29237.1"
                     /translation="IARRSRGTPRIANRILRRVRDFAEIEGTGIIDTAIAKHALSRME
                     IDEEGLDQMDRTILMTIIDLFRGGPVGIEAIAASVSEEKRTIEEVYEPYLMKMGYIAR
                     TPRGRVATARAFKKFGLEPDPKLQEPLF"
BASE COUNT          222 a          367 c          460 g          274 t
ORIGIN      
        1 gcggccgagg cggaggatcc cgacactctg caccggcacc ttttcctcgc cggctatcga
       61 caggtcgcgc agaacggcgt tgttcttgcg ctcctcgatg ggggaggtga taaagaccgc
      121 gcagaggtcg ttcagccatt tgtcggcgtc tagttgcata tcggcgggca cataggaaag
      181 ttcgttcagg atggccatga accgctcggc gatgagcgag ccggtgaccg tttcacgcag
      241 ttccacgatc atgtcgcgga gcgaataggc gtccgatccg aggaactcga ccggctggtc
      301 gagcgccgcg cgtacgagcg ccatgagttt cccgtggtat tcctgacggt cctcgcgcac
      361 catcaccggg gctacctctc gataccagag caacaggtcc atgatgccga cgagccgctt
      421 gtcgaatcgg ccggccagat ggcgggtgat gagcaggcgg taatcgcggc gggcgagaag
      481 cgcggggttg agggtccgtt cgcacagcgg gtacagcagg cgcatgtcct cgatgagtga
      541 tacgaacagg aaccgcggta cgaagtgggc gacattcttg tccacgaggt ttatcgtgtg
      601 ggtcacgatg tggtggaaca ccttgagttc gtgtttcaga tacccgaact cgtcgtcggt
      661 caccttctgc agtatcgtgt tgagttcgcg gttgatggtg ccgaacagcg gccggagcac
      721 cgtctgggtc ggttcgccca ccgtttcctt ggggttcttc agcagacgca tcgtgaggtc
      781 gatcgtgtag tcgagcgtgg tccagaagtc atagcggatg gtgtggagcg ccgatatctc
      841 ggtcttttgc cggtggatat aggggagcag tgcggcgcgg tccgggttct tgaacgaaga
      901 gagggcggtg aacgaccggt agtattcttt caaaacagcg gctcctgcag tttggggtcc
      961 ggctcgagcc cgaacttctt gaaggcgcgc gcggtggcga cccggccgcg cggggtgcgg
     1021 gcgatgtagc ccatcttcat gaggtagggc tcgtacacct cctcgatggt ccgtttctcc
     1081 tcgctgaccg acgcggcgat cgcctcgatg ccgaccgggc cgccgcggaa caggtcgatg
     1141 atggtcatga gtatcgtgcg gtccatctgg tcgagtcctt cctcgtctat ctccatgcgc
     1201 gacagggcgt gtttggcgat ggcggtgtcg atgatgccgg tcccctcgat ctcggcgaaa
     1261 tcgcggacgc ggcgcaggat gcggttggcg atgcgcggcg tgccacggct gcggcgggcg
     1321 atc
//
LOCUS       DAOQUE010000643         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_143830_length_1321_cov_5.015008, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000643 DAOQUE010000000
VERSION     DAOQUE010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143830_length_1321_cov_5.015008"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKH10_13795"
     CDS             complement(<1..605)
                     /locus_tag="PKH10_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNT29238.1"
                     /translation="MCGIAGFVGTGTRDDIRRMTGAMEHRGPDAEGFWHDPTAGVFFG
                     HRRLSILDIAGGAQPMSSADGKLVIVFNGEIYNHLELRRELEGEGARFSTDHSDTEVL
                     LHGYRLWGDDFVSRLNGMWSFAIYDRERGRLFLSRDRFGKKPLFYSLQRGVFAFASEL
                     SALRKHGALHLSHSEKALKKYFAYGYIPAPHTIFGEVSKLPG"
     gene            complement(607..>1321)
                     /locus_tag="PKH10_13800"
     CDS             complement(607..>1321)
                     /locus_tag="PKH10_13800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT29239.1"
                     /translation="HHASGEVMVFLDADLQNDPADIPALLDKLAEGYDVVSGWRKERK
                     DAFLNRTLPSMIANRLISEVTGCHLHDYGCTLKAYRREVLKGFTLYGEMHRFLPSYAY
                     QNGARIAELPVTHHPRTRGRSKYGIERTFKVMLDLMTVKFLSTYAHKPIYLFGVVGLT
                     LMTLAVLIVAYLIAMKFYLDISLIQSPLLLMSAMFVILGVQSIFLGLIAELLMRTYHE
                     SQDKPTYRIKESVNLAEND"
BASE COUNT          257 a          371 c          433 g          260 t
ORIGIN      
        1 cccggcagtt tcgacacctc gccgaatatg gtgtggggcg ccggtatgta gccgtaggcg
       61 aaatatttct tgagcgcctt ctcgctgtgc gaaagatgga gcgccccgtg tttccggagc
      121 gccgagagtt cggaagcgaa ggcgaaaacg cctctctgaa gcgagtagaa tagcggtttt
      181 ttgccgaacc ggtcacgcga gaggaagagc cgcccccgct cgcggtcgta gatggcgaac
      241 gaccacatcc cgttgagcct gctcacgaag tcgtcgcccc agagacggta gccatgcagg
      301 agcacctcgg tgtcggagtg atcggtagag aaccgggcac cttcgccttc cagttcccgc
      361 cgcagttcca gatggttgta tatctcgccg ttgaagacga tgactagttt cccgtcggcg
      421 ctcgacattg gttgggcgcc gccggcgata tcgaggatgg aaagccgccg gtgtccgaag
      481 aatactccgg ccgtcgggtc gtgccagaac ccttcggcgt cggggccgcg gtgctccatc
      541 gcgccggtca tacggcggat gtcgtcgcgc gttcccgttc cgacgaaccc ggcgatgccg
      601 cacatatcag tcgttttccg ccagattgac cgattccttg atccggtagg tcggtttgtc
      661 ctgcgattcg tggtaggtgc gcatcaacag ttcggcgatg agcccgagga atatcgactg
      721 tacgccgagt atcacgaaca tcgccgacat gagcagaagc ggcgactgga tgagcgagat
      781 atcaagatag aacttcatcg cgatgagata ggcgacgatg agcaccgcga gcgtcatgag
      841 cgtcagcccg acgaccccga acagatatat cggcttgtgg gcataggtcg aaaggaattt
      901 caccgtcatg aggtcgagca tcaccttgaa ggtccgctcg atgccgtact tcgatcgacc
      961 gcgcgtccgg ggatggtggg tgaccggcag ttcggcgatg cgcgcgccgt tctgataggc
     1021 gtaggagggg aggaagcggt gcatctcgcc gtagagcgtg aaccctttca gtacctcgcg
     1081 gcggtaggcc ttgagcgtac agccgtaatc atgcaggtga cagccggtca cctcggagat
     1141 gagacggttg gcgatcatag aggggagcgt gcggttgaga aaggcgtcct tgcgctcttt
     1201 gcgccagccg ctcaccacat cgtacccttc ggcgagttta tcgagcaggg ccgggatgtc
     1261 ggcgggatcg ttctgcaggt cggcgtcgag gaacaccatg acctcgccgc tcgcgtgatg
     1321 g
//
LOCUS       DAOQUE010000644         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_146042_length_1309_cov_3.339713, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000644 DAOQUE010000000
VERSION     DAOQUE010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146042_length_1309_cov_3.339713"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..>1309
                     /locus_tag="PKH10_13805"
     CDS             212..>1309
                     /locus_tag="PKH10_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29240.1"
                     /translation="MKKSFMVALVVLIACFFVSCDKMSAKPKETVKVDFHIMSQCPFG
                     VQVDTGIKPVLDKLGNAIEFNRHFIGQVKEDGTLTSMHGENEVKGNLLEICAAKHFPK
                     TYMNLVACMNKEYRKIPENFDACATEAKVDGAKIKACADGEEGKALLKASYGVSKEKG
                     ATGSPTIFINGERYKKGGRTENDFLRNICEVYKPENKPEPCKAIPAPKKVDLTVLTDA
                     RCTKCVVDPIVNQMKNIFPGLVEKKIDYGTEEGKKLYETLKATEARLLPAFLFDKNVT
                     EDPGFQQVQRWMLDVNDKKMLRVGGKFDPTAEICDNQQDDTGDGKIDCADETCKGNLV
                     CREEKKDRLDLFVMSQCPYGTKALDAMKEVLK"
BASE COUNT          342 a          337 c          383 g          247 t
ORIGIN      
        1 tccggctgac tgtggcggaa gtcgttatgg gcaaagacat gttcaaatga ttacaatatg
       61 ttgatgagtt ttattcggga tgttctgttg aaggagcggg gacaaaaaat tgacaatcac
      121 cgtaagtcgc gctatgagga gcggtcggtt gcagaaggat atgatgctct gcaagatgtt
      181 cccttatcaa catatcgtga ggaggaatcc cgtgaagaaa agtttcatgg tcgccttggt
      241 ggtgttgatc gcctgcttct tcgtgtcgtg cgacaaaatg agcgcgaagc cgaaagagac
      301 ggtcaaggtc gatttccaca tcatgtcgca gtgtcccttc ggcgtgcagg tcgataccgg
      361 gatcaagccg gtgctcgaca aactgggcaa cgcgatcgag ttcaaccgtc acttcatcgg
      421 tcaggtgaaa gaggacggaa cgcttacctc gatgcatggc gagaacgagg tcaagggcaa
      481 cctgcttgag atctgcgccg cgaaacattt ccccaagacc tacatgaacc ttgtcgcctg
      541 catgaacaag gaataccgga agataccgga gaacttcgat gcctgcgcca ccgaggcgaa
      601 ggtcgacggc gcgaagatca aggcgtgtgc cgacggcgaa gaagggaaag ccctgctcaa
      661 ggcctcctac ggcgtttcca aggaaaaggg cgcgaccggc tcaccgacca tcttcataaa
      721 cggcgaacgt tataagaaag gtggtcgtac ggagaacgat tttctccgca acatctgcga
      781 ggtctacaaa ccggagaaca agcctgagcc ttgcaaggcg ataccggcac ccaagaaggt
      841 cgatctgacg gtcctcaccg acgcccgttg caccaagtgt gtcgtcgatc cgatcgtcaa
      901 tcagatgaag aacatcttcc cgggccttgt cgagaagaag atagactacg gtaccgaaga
      961 gggtaagaaa ctgtatgaga ccctcaaggc gaccgaggcg cgcctgcttc ccgcgttcct
     1021 gttcgataag aatgtcaccg aagatcccgg gttccagcag gtccagcgct ggatgctcga
     1081 tgtgaacgac aaaaagatgc ttcgcgtcgg cggcaagttc gatccgaccg ccgagatatg
     1141 cgacaaccag caggatgaca ccggcgacgg caagatagac tgcgccgacg agacctgcaa
     1201 gggcaacctc gtctgccgcg aagagaaaaa ggaccggctc gacctgttcg tgatgagcca
     1261 gtgcccctac ggcaccaagg cgcttgacgc gatgaaagag gtgctcaag
//
LOCUS       DAOQUE010000645         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_146345_length_1307_cov_5.184505, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000645 DAOQUE010000000
VERSION     DAOQUE010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146345_length_1307_cov_5.184505"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PKH10_13810"
     CDS             complement(<1..587)
                     /locus_tag="PKH10_13810"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNT29241.1"
                     /translation="MEPTTARHQWIELSRSAYAHNILFFRRLLGTRAALAVVVKANGY
                     GHGLLETAGLAQENGVGMLCVHTLDEAQKLKASGFTGPILLLGPVPIARSEEAALCGA
                     ALVAYDEEQVAALERAAAATGRKVDIHLKVETGTHRQGVMPERLGALLDLLKRSPHLR
                     LAALYSHFANIEDTTDPSFALGQLRRFREACAAVE"
     gene            725..>1307
                     /locus_tag="PKH10_13815"
     CDS             725..>1307
                     /locus_tag="PKH10_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29242.1"
                     /translation="MTRAAFLALFGLALLLSFPAVAQTTTKKVAVLEIEDSSKSFDPS
                     LLSNATEMLRGRLSATGRFVVIDKSRQAEMLRKLVKESKQESWKECYDNSCRIQLGQA
                     LSADTMLRSTISCIGEECQLSCELVDLAKEATIHGAAAEFDKKDTKGLLEAIRVVVGS
                     LGGEPSARPYAAASAPCTGGTLSFKTSPAGVEVV"
BASE COUNT          240 a          422 c          405 g          240 t
ORIGIN      
        1 ttttccaccg ccgcgcatgc ctcgcggaac cggcgcaact gcccaagcgc gaacgacggg
       61 tcggtcgtat cctcgatgtt ggcgaagtgg ctgtagagcg ccgcaagacg caggtgcgga
      121 gaacgcttga gcaggtcgag cagggcgccg agtcgttcgg gcatgacccc ctgccggtgg
      181 gtgccggtct cgaccttgag gtggatgtcc accttccgcc ccgtcgccgc agccgcccgt
      241 tcgagcgcgg ccacctgctc ctcatcgtag gcgacgagcg ccgccccgca gagcgccgcc
      301 tcctcgctcc gggctatcgg cacgggaccg agaagcagga tgggccccgt gaagcccgac
      361 gccttgagtt tttgcgcctc gtcgagcgta tggacgcaca gcatcccgac gccgttctcc
      421 tgcgcgagcc ccgccgtttc gagcagaccg tggccgtacc cgttcgcctt gacgacgacc
      481 gcgagcgcgg cgcgggtccc gagaagacgg cggaagaaca agatgttgtg cgcgtaggcg
      541 ctccgcgaaa gttctatcca ctgatgccgt gcggtcgtcg gttccatgtg ccctccttcc
      601 gtgaacgggc gcattatatg ccacacgaag gacgaagcaa taaaaaacgg ggagttatct
      661 tgcttttacc catgatgtat cctagacttg gccgctgtgt gatgctgatc aacgggggta
      721 cacgatgaca cgggccgcat ttcttgctct attcgggctg gcgctcctgc tctcttttcc
      781 cgctgtcgcg caaacgacga caaaaaaggt cgcggtgctt gagatagagg attcctcgaa
      841 aagtttcgat ccctccctcc tttccaacgc gaccgagatg ctccggggcc ggctcagcgc
      901 gaccggacgc ttcgtcgtca tcgacaagtc acgacaggcc gagatgctca ggaaactcgt
      961 caaggagtcg aaacaggaat cgtggaagga gtgttacgac aactcctgcc gcatacagtt
     1021 gggacaggcg ctttcggccg acaccatgct ccgctccacg atatcgtgca tcggcgagga
     1081 gtgccaactg tcgtgcgaac tggtcgatct cgcgaaggag gcgaccatcc acggcgccgc
     1141 cgcggagttc gacaagaagg acaccaaagg attgctcgag gcgatccgcg tcgtcgtcgg
     1201 ttcgctcgga ggcgaacctt cagcgcgccc gtatgccgcg gcttccgctc cctgcaccgg
     1261 cggcacgctc tctttcaaga cctcaccggc cggcgtcgag gtagtgc
//
LOCUS       DAOQUE010000646         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_148985_length_1293_cov_4.247981, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000646 DAOQUE010000000
VERSION     DAOQUE010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148985_length_1293_cov_4.247981"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /gene="mraY"
                     /locus_tag="PKH10_13820"
     CDS             <1..929
                     /gene="mraY"
                     /locus_tag="PKH10_13820"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNT29243.1"
                     /translation="MEQAIRDDGPQAHLAKAGTPAMGGVIILFSILVNALLWTDFIGN
                     FWVQAMSLLIVLFGLIGFLDDWLKVSKHNPKGLASRWKFLLQILFATIFMVLVYRHSG
                     FSPLLVVPIFKNLVFDIGWGYIPFGALVIVATSNALNLTDGMDGLATVPTVVSMTLLA
                     VVVYLVGHAEFASYLHLPHIRGAGELAVIGGATIGASLAFLWFNSYPASIFMGDTGAL
                     ALGGMIGAFTVVTKTELLSLIFNGLFVVEALSVIVQVSFFKTTGRRVFRMAPIHHHFE
                     LGKGANEPKLIVRFWILSILLALFSIAMIKVR"
     gene            934..>1293
                     /gene="murD"
                     /locus_tag="PKH10_13825"
     CDS             934..>1293
                     /gene="murD"
                     /locus_tag="PKH10_13825"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNT29244.1"
                     /translation="MKKALILGLGISGIETAKFLAAKKWDVVVHDDNAAKLAEAGKGF
                     ATLDGAMLPDRLDLLVISPGVPREHPLVVTAAARGVDIAGEMEVAARFLRDEKVIAVT
                     GTNGKSTTVTLIHEMLLK"
BASE COUNT          226 a          386 c          400 g          281 t
ORIGIN      
        1 atatggagca ggcgatacga gatgacggcc cgcaggctca tctcgccaag gcggggaccc
       61 cggcgatggg tggcgtcata atcctgttct cgatactcgt caacgcgctc ctttggaccg
      121 attttatcgg taacttctgg gtgcaggcga tgtcgctcct catcgtattg ttcgggctca
      181 tcgggttcct cgacgactgg ctgaaggtct cgaagcataa tccgaaaggg ctcgcctcgc
      241 gctggaaatt ccttttgcag atactgttcg cgacgatctt tatggtgctt gtgtaccgcc
      301 acagcggttt ctccccgttg cttgtggtgc ccatattcaa gaacctcgtg ttcgatatcg
      361 ggtggggcta catcccgttc ggcgcgctcg tcattgtcgc gacgagcaac gcgctcaatc
      421 tgaccgacgg catggacggg ctcgcaacgg tgccgacggt cgtgtcgatg actttgctcg
      481 ccgtcgtcgt gtatctcgtc ggtcacgccg agttcgcctc ctacctgcac ctgccgcata
      541 tccgcggtgc gggagaactc gcggtcatcg gcggcgccac tatcggcgcg tcgctcgcgt
      601 tcctgtggtt caacagttac cccgccagca tcttcatggg cgacaccgga gcgctcgcgc
      661 tcggcggcat gatcggcgct ttcacggtcg tcaccaagac cgaactgctg tcgcttatct
      721 tcaacgggct gttcgtcgtc gaggcgctgt cggtcatcgt gcaggtctcg ttcttcaaaa
      781 cgacggggcg ccgcgtcttc cgcatggcgc cgatacatca ccacttcgaa ctcggcaagg
      841 gggcgaacga gccgaaactc atcgtccgtt tctggatact ttccatactg ctcgctcttt
      901 tttcgatagc gatgatcaag gtgcggtgac gacatgaaaa aggcgctcat actgggactc
      961 ggcatatcgg gtatcgaaac ggcgaagttc ctcgcggcta agaaatggga tgtcgtcgtc
     1021 catgacgaca acgcggcgaa actggccgag gcggggaagg gcttcgcgac gctggacggc
     1081 gcgatgttgc ccgaccggct cgacctgctc gtcatcagtc ccggcgtgcc gcgcgaacat
     1141 ccgctcgtcg tcacggcggc ggcgcgcggc gtcgatatcg ccggcgagat ggaggtcgcg
     1201 gctcggttcc tgcgggacga gaaggtgatc gcggtgaccg gcacgaacgg caagagtacg
     1261 accgtgacgc tgatacacga gatgcttctg aag
//
LOCUS       DAOQUE010000647         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_149002_length_1293_cov_3.417609, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000647 DAOQUE010000000
VERSION     DAOQUE010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149002_length_1293_cov_3.417609"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PKH10_13830"
     CDS             <1..944
                     /locus_tag="PKH10_13830"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HNT29245.1"
                     /translation="GTTSVTVKYSAASRAGTGVAAQAAGSTATYSLNQYQVLNLNSAG
                     PGCGDSTCYGADLTGSFIEADKRVAVFGGHMCTYVPAGSPACDHLEHQIFPLRSWGMN
                     YAAVRTKPRGSEADYWKILAAQDGTSVSWSGGVTGSVTLNAGQAHQLSTTADFVVTAD
                     KPILLAQILASQDAGAGTGDPALMLTAPNEQFRKDYIFLVAPNYDYDRLTIVAQADTN
                     ITLDSTSMSSNTFTAIAGTSWLRQYVDTADGAHKLTADKPVGLYVYGFSQYVSYAYTA
                     GLDLVEIYSCWDLNSNGTCDIATEDLSGDGVCNERDC"
     gene            1053..>1293
                     /locus_tag="PKH10_13835"
     CDS             1053..>1293
                     /locus_tag="PKH10_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29246.1"
                     /translation="MKTIIFPVAALSLFFFACSSDVAKILNDDLLIGDGVNIPDDNGN
                     DGITKDDGPLVNDDGQLINDDGQVINDDGQVINDDG"
BASE COUNT          267 a          434 c          351 g          241 t
ORIGIN      
        1 cggggaccac ttcggtgacg gtcaaataca gtgcggcatc acgagccggc accggcgtcg
       61 cggcgcaggc ggccggaagc accgccacct attcgctcaa ccaatatcag gtgctcaacc
      121 tcaactcggc gggccccggt tgcggcgaca gcacctgcta tggcgccgac ctcaccggca
      181 gtttcatcga ggccgacaag agggtcgccg tcttcggcgg ccacatgtgc acctatgtgc
      241 cggccgggtc tcccgcctgc gaccacctcg aacaccagat attcccgctc cgctcgtggg
      301 gtatgaacta cgccgcggtc cgcaccaaac cacgcggttc cgaggccgat tactggaaga
      361 tactcgccgc gcaggacggc acaagcgtat cgtggagcgg tggcgtgacc ggttcggtaa
      421 ccctcaatgc cggacaggcc caccaactct cgacgaccgc cgatttcgtc gtgaccgccg
      481 acaaaccgat attgctcgcc cagatactcg cctcgcagga tgccggcgcg ggcaccgggg
      541 acccggcgct gatgctcacc gcgccgaacg agcagttccg caaggactac atattcctcg
      601 tcgcccccaa ctacgattac gaccggctca ccatcgtcgc gcaggccgat accaacatca
      661 cgctcgacag cacctcgatg agttcgaaca ccttcaccgc gatagccggc acctcgtggc
      721 tgcgccagta tgtcgatacg gccgacggcg cccacaaact gaccgccgac aagccggtcg
      781 gcctctatgt gtacggcttc tcgcagtatg tctcctacgc ctacaccgcc ggactcgacc
      841 tcgtcgagat atacagttgc tgggatctca acagcaacgg cacctgcgac atcgcgaccg
      901 aggacctgtc gggcgacggc gtctgcaacg aacgcgactg ctgatccccc cacttattgt
      961 tgtcccccca tattttcgtt tgactttccc cttttcttca cctataatcg ccgcgtcggt
     1021 tcgatttaac catgtgtaag ggggcaaaac ccatgaaaac gatcatcttt ccggtcgcgg
     1081 cgctgagtct gttctttttc gcctgttctt ccgatgtggc gaagatactg aacgacgacc
     1141 ttctcatagg cgacggcgtg aatatccccg atgataacgg gaacgacggg atcaccaaag
     1201 atgatggccc gctggtcaac gatgacggcc agttgataaa cgacgacggt caggtcatca
     1261 atgacgacgg tcaggtcatc aatgacgacg gtc
//
LOCUS       DAOQUE010000648         1287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_150107_length_1287_cov_4.280844, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000648 DAOQUE010000000
VERSION     DAOQUE010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150107_length_1287_cov_4.280844"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PKH10_13840"
     CDS             <1..759
                     /locus_tag="PKH10_13840"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNT29247.1"
                     /translation="DARRAHRFGAGMTMRTVAIALLALLIAACADPYGIYHKVRDKED
                     IQKISLLYKVDIGKIRSANSMGGADEVKPGDYLFIPGVRAPMDGTAVTVSSRKKETGA
                     TVGTGKQALATTHGAKGKDLKATGDAKFSWPLKGVVTSPFGPRDGKMHEGIDISVPER
                     TPVFAAGGGKVIFSADHGGYGNVVIIQHADNYFTVYAHNRELLVKEGQTVKQGDKIAL
                     SGNTGRSSGPHLHFEVRIGAKPQDPMQYLPRAAP"
     gene            750..828
                     /locus_tag="PKH10_13845"
     tRNA            750..828
                     /locus_tag="PKH10_13845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Arg,seq:cct)
     gene            904..>1287
                     /locus_tag="PKH10_13850"
     CDS             904..>1287
                     /locus_tag="PKH10_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016708527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HNT29248.1"
                     /translation="MALVFSNFKRALTSLEKALALPKDDIVRDSVVKRFEYSYELAWK
                     LLQRALEEDLGSAAVDGLPRRELFRIGAEKQLIADVVPWFEYHRLRNLSSHTYNEEIA
                     EEMYAAAGRFAVDARLLLKKLESRNG"
BASE COUNT          263 a          393 c          418 g          213 t
ORIGIN      
        1 gacgctcgac gcgctcatcg gttcggtgca ggaatgacga tgcggacggt cgccatcgcc
       61 ctgctcgcgc tgcttatcgc cgcctgcgcc gacccctacg gcatctacca caaggtgcgc
      121 gacaaggagg acatccagaa gatatcgctc ctctacaagg tggacattgg caagatacgc
      181 tcggcgaaca gcatgggcgg cgccgacgag gtgaagccgg gcgactacct gttcatcccg
      241 ggcgtccgtg ccccgatgga cggcaccgca gtgacggtct cgtcgcggaa aaaggagacg
      301 ggcgcgacgg tgggtaccgg aaaacaggcg ctcgcgacga cccacggcgc gaaggggaag
      361 gatctcaagg cgaccggcga cgcgaagttc tcgtggccgc tcaagggggt ggtcacctcg
      421 ccgttcggtc cgcgcgacgg taagatgcac gaggggatag acatcagcgt gccggagagg
      481 acgccggtct tcgcggcggg cggcggcaag gtgatattct cggccgatca cggcgggtac
      541 ggcaatgtcg tcatcatcca gcacgccgac aactacttca cggtgtacgc ccacaaccgc
      601 gaactgcttg tgaaagaggg gcagacggtc aaacagggcg acaagatagc attatcgggc
      661 aacaccgggc ggtcaagcgg cccgcacctc catttcgagg tgcgcatcgg cgccaagccg
      721 caggacccga tgcagtacct tccccgggcg gccccatagc tcagatggat agagcggcgg
      781 attcctaatc cgcgtctgcc gcaggttcga ctcctgctgg ggccaccact cctttaacga
      841 aatttgcatt ttcgcgttgc atccggtagc attcgatcgg tcgtacgcgg aaaggaggcg
      901 agcatggctt tggtgttcag caacttcaaa cgggcgctca cctcgctcga aaaggcgctc
      961 gccctgccga aggacgatat cgtccgcgac tcggtggtca agcggttcga atattcctac
     1021 gaactggcgt ggaaactgct ccagcgggcg ctggaagagg atctcggaag cgccgcggtc
     1081 gacggcctgc cgcgccggga actgttccgc atcggcgccg agaaacaact catcgccgat
     1141 gtggtcccgt ggttcgagta tcaccggctc cgcaatcttt cgagccacac ctacaatgaa
     1201 gagatagccg aggagatgta tgcggcggcg gggcgtttcg ccgtcgatgc gcgcctgctc
     1261 ctcaagaagc tcgaatcgcg caatggc
//
LOCUS       DAOQUE010000649         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_152336_length_1276_cov_3.813268, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000649 DAOQUE010000000
VERSION     DAOQUE010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152336_length_1276_cov_3.813268"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PKH10_13855"
     CDS             complement(<1..217)
                     /locus_tag="PKH10_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29249.1"
                     /translation="MSKGVLLFVRGDGKRTDEIAQAVALVRDLATELGRPVLISPDKT
                     FDIGLIVAIGGDGTFLDASHEALSLDVP"
     gene            complement(214..>1276)
                     /locus_tag="PKH10_13860"
     CDS             complement(214..>1276)
                     /locus_tag="PKH10_13860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNT29250.1"
                     /translation="AVAVLDGLDYAHNTRDRNGNFLKVVHLDMNPNNILLSYDGKIKI
                     VDFGIANAVYTKVLKNDLRTVQGTYGYLSPEQCQEKELDRRSDLFSFGVILYELLSGQ
                     PLFKHLDSDAAILHAIVHNEVPPVHTVNPEVPRELGFILQKALQKTRERRYGSAAEML
                     DDIKRFQSMRELNPQVDTLPAILKREFAAHFIKMTKVLEKAQTAYLVDEIFKDIGEIE
                     QIDLNEKIRIQQDDRSRRPAEPEEPEDDIKKGWKWPIIISLVLLALFFTGLGVYLFFP
                     FESETVTVFISSRPTEADIYLDGRESGNLTPTEMTLTKGRTYIFEFRKGDLVAELPFT
                     PGDKPEDNKIFVELKGRQP"
BASE COUNT          242 a          364 c          390 g          280 t
ORIGIN      
        1 tggggacatc gagcgaaagc gcctcgtgac tcgcgtcgag gaaggtgccg tcgccgccga
       61 tggcgacgat gaggccgata tcgaaggtct tgtcgggcga tatgaggacc gggcgcccca
      121 gttcggtcgc aaggtccctg acgagcgcga cggcctgcgc gatctcatcc gtgcgtttcc
      181 cgtcgcccct gacgaacagg agcacgcctt tgctcatggc tgtcttccct tcagttcgac
      241 gaatatcttg ttgtcctcgg gcttgtcacc cggtgtaaaa ggcagttcgg cgaccagatc
      301 gcccttgcgg aactcgaaga tataggtgcg cccctttgta agcgtcatct cggtcggcgt
      361 caggttgccg ctctcgcgac cgtcaagata gatgtcggcc tcggtcggac gcgaggagat
      421 gaagaccgtc accgtctcgc tctcgaaggg gaagaagaga tacacgccga gacccgtgaa
      481 aaagagcgcg aggagcacga gcgagatgat gatcggccac ttccatccct tcttgatgtc
      541 atcctccggc tcctccggct ccgcagggcg gcggctccga tcatcctgct ggatgcggat
      601 cttctcgtta aggtctatct gctctatctc gccgatatcc ttgaatatct cgtcgaccag
      661 atacgcggtc tgcgcctttt cgagcacctt ggtcatcttg atgaagtggg ccgcgaattc
      721 ccgtttgagg atggcgggca gggtgtcgac ctgcggattg agttcgcgca tactctggaa
      781 ccgcttgatg tcgtcgagca tctcggcggc gctcccgtag cgacgctccc gcgtcttctg
      841 gagcgccttc tggagtatga agccgagttc gcgaggcacc tcggggttga ccgtatgcac
      901 aggcggcacc tcgttgtgga cgatagcgtg cagtatcgcc gcatccgagt cgagatgctt
      961 gaaaagcggc tgacccgaaa gcagctcgta gaggatgacg ccgaaactga acagatcgct
     1021 ccggcgatcg agttcctttt cctgacactg ttcgggagag agatacccgt aggtgccctg
     1081 caccgtacgc aggtcgttct tgagcacctt ggtgtagacc gcgttggcga taccgaaatc
     1141 gacgatcttg atcttgccgt catacgagag caggatattg ttgggattca tatcaagatg
     1201 gacgaccttg aggaaattgc cgttacggtc acgggtattg tgggcgtaat cgagcccgtc
     1261 cagcaccgct accgct
//
LOCUS       DAOQUE010000650         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_152351_length_1276_cov_3.324324, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000650 DAOQUE010000000
VERSION     DAOQUE010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152351_length_1276_cov_3.324324"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PKH10_13865"
     CDS             <1..875
                     /locus_tag="PKH10_13865"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNT29251.1"
                     /translation="GGKFRLCPLRTGTVNALTFFPRLYRLDVAVVTFGAALAGPYFST
                     GSFSSVDVTIAAFISLILYNYVYVLNALTDRTGDTISHPERPLPSGRLGIMPAACYLV
                     LLLHLAVAGSIALFTGPSLFLALLVVFLGTLYSAAPFELKKHPFVAIAVTAWGMAHPF
                     FITAHTAKTSLGISFILVALGVVAFKDIGDPGGDVVAGRRSVLAVLSLRTLCLLSLLS
                     FVAAAIALLLLDLPVLLMLPAFTGATLLYHLTHGALEKSLPLLYTRLIHSALAAIVGI
                     IVVVVADLLCAGGT"
     gene            872..>1276
                     /locus_tag="PKH10_13870"
     CDS             872..>1276
                     /locus_tag="PKH10_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29252.1"
                     /translation="MNLRSLLIGERPSIHWRMLAFFMVFELFAAGRVFVEMRILADNT
                     SMIVALHRFLWYTYVFLFFAVCYRRILHAPFERLNLLALGGVILFIPPLYALLGGYEF
                     RLNYLVSSDPFEIARELLTLHYGHEKNFLMFPE"
BASE COUNT          191 a          456 c          312 g          317 t
ORIGIN      
        1 gaggcgggaa gttccgcctt tgcccgttgc gtacgggaac cgtgaacgcc ctcactttct
       61 tcccccgttt gtaccgtctc gatgtcgccg tggtcacctt cggcgccgcg ctcgccggcc
      121 cttacttctc gaccggctcc ttctcgtcgg tcgatgtcac catcgccgcc ttcatatcgc
      181 tcatactcta caactatgtc tatgtcctca acgcactgac cgaccggacc ggagacacga
      241 taagtcaccc cgaacgacca ctcccctccg gtcgactcgg catcatgccg gcggcgtgtt
      301 atctcgtcct gctcctccat ctggcggtcg ccggttccat agcgctgttc accggcccct
      361 cgctcttcct cgcgcttctg gtcgtcttcc tcggcaccct ctactcggcg gcaccgttcg
      421 aactcaagaa acatcctttc gtcgccatcg cggtgaccgc atgggggatg gcgcatccct
      481 tcttcatcac cgcccataca gcaaaaacct ctctcggtat ctccttcata ctcgtcgctc
      541 ttggcgttgt agcgttcaag gacatcggtg accccggcgg agacgtcgtg gccggtcgcc
      601 gatccgttct tgcggtgcta tcattgagaa cgctctgcct cctctcgctc ttatccttcg
      661 ttgcggcagc gatcgccctt ctcctgctcg atctgccggt cctgctcatg ctaccggcct
      721 tcaccggcgc aacgctcctc tatcatctta cgcatggcgc gcttgaaaaa tcgttgcctc
      781 tcctgtatac tcggctcatc cacagcgccc ttgcggcgat cgtcggcatc atcgtcgtcg
      841 tggtggcgga cctgctctgc gcgggaggaa catgaacctg cgatcgctcc tcatcgggga
      901 acgcccgtcg atccactggc ggatgctcgc cttcttcatg gtgttcgaac tgttcgccgc
      961 cggtcgggtg ttcgtcgaga tgcggatact cgccgacaat accagcatga tcgtggcact
     1021 ccaccgtttc ctctggtaca cctatgtctt cctctttttc gcggtgtgct atcggcgcat
     1081 actccacgca ccctttgaac ggcttaacct gctcgccctc ggcggcgtca tactcttcat
     1141 ccctccgctc tacgcacttt tgggcggcta cgagttccgg ctcaactacc tcgtatcgag
     1201 cgatccgttc gagatagcgc gggaactgct cacgctccac tacggtcacg agaagaactt
     1261 cctcatgttc cccgag
//
LOCUS       DAOQUE010000651         1258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_156106_length_1258_cov_3.384871, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000651 DAOQUE010000000
VERSION     DAOQUE010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156106_length_1258_cov_3.384871"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..>1258)
                     /locus_tag="PKH10_13875"
     CDS             complement(219..>1258)
                     /locus_tag="PKH10_13875"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT29253.1"
                     /translation="SVQTSKGHFAVFFSVVPSIRGKVYTDYPRTTFHSLPKALSDAGY
                     ETVFFQSYKDLSFDNTRPFLSGNGFARVETVASFTTPEDEPLVWGWGLQDDAVYRKFF
                     AYLDALQEKGEARRPYFGAVATISAHGEFDQVPRHLRTLYPDPSDRRERFANALHASD
                     EFLKTFFAELRRRPYLDNSIVVITGDHSVPNGRHGLFCGESAAYEEFFRVPLLVLWPG
                     RLKPERIPDIPFSQIDIAPTLAALAGVPMPWHHFEGRSLLAERRVEPVYLVQPYNGQF
                     LSVVEYPHKLTRHLAGGTVMLFDLKADPMEERDLSGDPAFADVRTRLERSLSFIFLNQ
                     ELIRHDEVWRK"
BASE COUNT          291 a          344 c          400 g          223 t
ORIGIN      
        1 ggtaggaaac aagattcata gccagcgcga tgatgaaaaa gatggtcgcg ccgtcgggcc
       61 cgcctagcag atagccgaaa aatacgagca ggagcgtcat gacgatcaac agcaggccgc
      121 ttttgaaaag gttgtacatg gtatcggatc ctccgttaac atgtcatcga tgcatacata
      181 taagcatcaa gggcagaaaa tcaaatgggc acgatgcgtc attttcgcca cacctcgtca
      241 tggcggataa gttcctgatt gaggaagatg aaggagagcg accgttcgag ccgcgtccgc
      301 acatcggcaa aggcgggatc acctgacaga tcacgttctt ccatcgggtc ggccttcaga
      361 tcgaacagca tcacggtacc gccggcaagg tgtcgggtca gtttgtgggg gtactccacg
      421 acgctcagga attggccgtt gtagggctgc acgagataga ccggctcgac gcgccgctcc
      481 gcgagcaggg agcgtccctc gaaatggtgc cacggcatcg ggacgccagc gagtgccgcg
      541 agcgtcggcg ctatgtcgat ctgcgaaaag gggatatcgg gtatccgttc cggcttgagg
      601 cgaccgggcc acagcacgag caacggcacg cgaaagaact cctcgtaggc ggccgactca
      661 ccgcagaaaa ggccgtgccg accgttcggc acgctgtgat cgccggtaat gacgacgatg
      721 ctgttatcga gatacggacg acggcgcagt tcggcgaaga atgtcttaag gaactcgtcg
      781 ctcgcgtgca gagcattggc gaaccgttcc cgacgatccg acggatctgg gtacagcgtc
      841 cgcagatgac gcgggacctg atcgaactcg ccgtgcgcgc tgatggtggc aactgcgccg
      901 aaatagggtc gccgcgcctc ccccttttcc tgcaatgcgt caagataagc gaagaacttg
      961 cggtacaccg catcgtcctg cagcccccag ccccagacga gcggctcgtc ctcaggtgtc
     1021 gtgaaggagg cgaccgtctc gacacgcgcg aacccgttgc cggagaggaa gggacgggta
     1081 ttgtcgaagg agagatcctt gtaggattgg aagaacaccg tttcgtagcc ggcatccgag
     1141 agcgccttgg gcaacgaatg gaatgtggtg cgcgggtagt cggtgtagac tttgccgcga
     1201 atgctcggga cgaccgagaa aaagaccgcg aagtggcctt tcgatgtctg caccgaat
//
LOCUS       DAOQUE010000652         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_157728_length_1250_cov_3.506276, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000652 DAOQUE010000000
VERSION     DAOQUE010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157728_length_1250_cov_3.506276"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PKH10_13880"
     CDS             complement(<1..890)
                     /locus_tag="PKH10_13880"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNT29254.1"
                     /translation="MILRTVFFLLTVAVMAWLFPEWRPILPPLAAIGLAFLTRRVIVA
                     LFCGIWVGAAILLSRDGGTMLASVGGGFFRAADTWVIKGLNDPSHLLIIVFTALIGGM
                     TGILAASGGIHALVALIARKAKNRVGAQLAAYATGVLMFFDDYSSTIVTGSAFRPLTD
                     RWRVSREKLSFIVDSTSAPIASVSILSTWIGYELGLIADAFKQNNIDLEAYNAFLASI
                     PYRFYALLLLAMVPISALMRREFGPMRRAEERTLTTGVPHEPGGDLAAGADESQLPPA
                     DKRRWYDAVLPVAVMVLSVLG"
     gene            complement(887..>1250)
                     /locus_tag="PKH10_13885"
     CDS             complement(887..>1250)
                     /locus_tag="PKH10_13885"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT29255.1"
                     /translation="AIMMEQGELPRDAKQVLATYDKACDKGDARACSNAGFMYINGDG
                     APLDQEMGRALFEKSCTMKHGEGCYNLGNVHESGAGVEKDTAKALEYYTLACSLDHKK
                     ACDAVKAAKNPKKGGKKK"
BASE COUNT          216 a          384 c          422 g          228 t
ORIGIN      
        1 ccgagcaccg acaggaccat cactgcgacc ggaaggaccg cgtcgtacca gcgccgtttg
       61 tcggcgggcg gaagttgcga ctcgtcggcg cccgcggcga gatcgccgcc cggttcgtgg
      121 ggaacgccgg tggtcagtgt ccgttcttcg gcgcggcgca tcgggccgaa ctctcgtcgc
      181 atcagggccg agatcggcac catcgcgagc aggagcagcg cgtagaaacg gtagggtatc
      241 gaggcgagaa atgcgttgta ggcctcgaga tcgatgttgt tctgtttgaa ggcgtcagcg
      301 atgagcccca gttcgtagcc gatccaggtc gaaagtatcg agaccgaggc gatgggagcg
      361 ctcgtcgaat cgacgatgaa ggagagtttc tcgcggctca cgcgccagcg gtcggtgagc
      421 gggcggaagg ccgacccggt gacgatggtc gatgagtagt cgtcgaagaa catcaggacg
      481 ccggtcgcgt aggcggccag ttgcgcgccg acccggttct tggccttgcg ggcgatgagg
      541 gcgaccagtg catggatgcc gcccgacgcg gcgagtatcc cggtcatccc gccgatgagc
      601 gcggtgaaga cgatgatgag gagatgtgac gggtcgttga gccctttgat gacccatgtg
      661 tcggcggcgc ggaaaaagcc cccgccgacg ctcgcgagca tcgtcccgcc gtcgcgtgac
      721 agcaggatcg ccgccccgac ccagatgccg cagaaaagcg ccacgatcac ccgccgcgtg
      781 aggaacgcaa gcccgatcgc ggcgagcggc ggcagtatcg gtcgccactc cgggaacagc
      841 cacgccatga cggcgacggt aagcaggaag aagacggtgc gcagtatcat ttctttttcc
      901 cgcccttctt cgggttcttc gccgccttga cggcgtcgca ggccttctta tggtcgagcg
      961 aacaggcgag cgtgtagtat tcgagcgcct tggcggtatc cttttcgacc cccgccccgc
     1021 tttcgtggac attgcccaga ttgtagcatc cctcgccgtg tttcatggtg cacgacttct
     1081 cgaacagcgc ccgtcccatt tcctgatcga gcggcgcgcc gtcgccgttg atgtacatga
     1141 acccggcgtt cgagcaggcg cgggcgtcgc ctttgtcgca ggccttgtcg taggtcgcga
     1201 gcacctgctt ggcgtcgcgc ggcagttctc cttgctccat cataatggct
//
LOCUS       DAOQUE010000653         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_159065_length_1244_cov_2.918419, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000653 DAOQUE010000000
VERSION     DAOQUE010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159065_length_1244_cov_2.918419"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1244
                     /locus_tag="PKH10_13890"
     CDS             <1..>1244
                     /locus_tag="PKH10_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29256.1"
                     /translation="EASVDIPADALDQDTEITIEKLSKDGRPESGKLSSAVWKFGPEG
                     LVFKKEVTMTIKIDAAKEAGPVAVVAVERDGGWHSAGYSNGTGAVGAAAAGDPIMAGD
                     PIMAGDPIMAGDPIMSQVGHFSNFAVYVVGVTGDKSCVELINCAFPCGMDLECASACR
                     EQGTAEAQAQFDALRSCGEEQCGEFCMSADTQEECKTCSDEKGATEVAACGLYDDNDD
                     DAIHTEEDPVSDDAGPTDNETKEVDPTDEDALLPDELVVLDPQDLLGEWDFDGGLSHI
                     SIMWNEEEQEYMLDMSFNDYSEFYMSQPGGSFTEHTYTGGYSYWDQSEQQQFGNMVAV
                     FTLHTDSGLLDVDITGDAPLAPRALTGGVRPVYDDTPLPDDDMIVGEDDLIAPDDDEV
                     VIPTCGNGILESGETCDPPGSG"
BASE COUNT          250 a          354 c          426 g          214 t
ORIGIN      
        1 gggaggcatc ggtcgacata ccggccgacg cgctcgacca ggacaccgag ataacgatag
       61 aaaaactctc gaaggatggg cgccccgaga gcggcaaact ctccagtgcg gtctggaagt
      121 tcggccccga ggggctggtg ttcaagaaag aggtcacgat gaccatcaag atcgacgccg
      181 ccaaggaggc ggggccggtg gcggtcgtcg cggtggagcg ggacggcggg tggcattcag
      241 ccgggtacag caacggcacc ggcgcggtcg gcgcggcggc ggcgggcgat ccgatcatgg
      301 cgggcgatcc gatcatggcg ggcgacccga tcatggcggg cgatcccatc atgtcgcagg
      361 tcggtcactt ctcgaacttc gcggtctatg tcgttggtgt gacgggcgac aagagttgcg
      421 tggaactgat aaactgcgcg ttcccgtgcg gtatggatct tgagtgtgct tcggcctgtc
      481 gcgaacaagg caccgccgag gcgcaggcgc agttcgatgc gctccgttcc tgcggcgagg
      541 agcagtgtgg cgaattctgt atgtcggccg atacgcagga ggagtgcaag acctgttcgg
      601 atgagaaggg cgccaccgag gtcgcggctt gcggcctgta cgatgataac gacgacgatg
      661 cgatacacac cgaagaggat ccggtgtccg acgatgccgg cccgaccgac aacgagacga
      721 aggaggtcga cccgaccgat gaggacgctc ttctgcccga cgaacttgtg gtactcgacc
      781 cgcaggatct gctgggcgag tgggattttg acggtggcct gagtcacatc agtatcatgt
      841 ggaatgagga agagcaggaa tatatgctcg acatgagttt caatgactac agcgagttct
      901 acatgtctca acccggcggc tccttcacgg agcacaccta caccggcggg tattcctact
      961 gggatcagtc ggaacagcag cagttcggca acatggtggc ggtcttcacg ctccataccg
     1021 acagcggcct gctcgatgtc gacatcaccg gcgatgcgcc gctggcgccg cgtgccctca
     1081 ccggcggcgt ccgtccggtc tatgatgaca cgccgcttcc cgacgacgat atgatagtgg
     1141 gagaggatga tctgatagcg cccgacgacg atgaggtcgt catccccacc tgcggcaacg
     1201 gcatcctcga gtcgggcgag acctgcgacc cgccgggatc gggt
//
LOCUS       DAOQUE010000654         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_159280_length_1243_cov_2.771886, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000654 DAOQUE010000000
VERSION     DAOQUE010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159280_length_1243_cov_2.771886"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1243
                     /locus_tag="PKH10_13895"
     CDS             <1..>1243
                     /locus_tag="PKH10_13895"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNT29257.1"
                     /translation="NTAKSGKGMNDNYQYAAYHSYWPIATGWTDTDPLSGLSSPLEDH
                     FGTDAELRELVQKAHAKGIRVLIDLVANHVHTDSPLWIEHKNDGWFNMAPDGKTPNVN
                     GGYNCGWDAPIECWFTDYLPDFDYRDKELLDLVLDHAVWLVHEYDFDGFRMDAVKHMV
                     MGFTTGTRSRIEQDIVTTGIPFYTVGETFDGDKMKIAEYVGADKLDGQFDFPLYFQVR
                     DILLQHSRGMNELASFVAANDTYYTDRWNGAIMSNFMGNHDVTRALTFAGGDHARLRM
                     AQTFLLTSPAIPLIYQGDDIGMEGGEDPDNRRMMVFEPSLSTDQRATLAHLKKLGMAR
                     KAHPALRTGIRTTLISETNLWAYRMKSGADEAIVILNRGAQTQRQLSTTLTGTLTDEI
                     SGNTAAVSGGKITVTVPAMTSA"
BASE COUNT          271 a          414 c          357 g          201 t
ORIGIN      
        1 agaacaccgc gaagtcgggt aaagggatga acgacaacta tcagtacgcg gcctaccatt
       61 cctactggcc gatcgcgacc ggttggaccg acaccgaccc gctgtcgggt ctttcctcgc
      121 cgctggagga ccatttcggg accgatgccg aactgcgcga actggtgcag aaggcgcacg
      181 ccaagggcat ccgcgttctc atcgatctcg tcgccaacca tgtccacacc gacagcccgc
      241 tgtggataga gcataagaac gacggctggt tcaacatggc gcccgacggg aagacgccca
      301 atgtgaacgg cggctacaac tgcggctggg acgccccgat cgaatgttgg ttcaccgact
      361 atctgcccga cttcgattac cgcgacaagg aactgctcga tctggtactc gaccacgcgg
      421 tatggctggt ccacgaatac gacttcgacg gtttccggat ggacgcggtc aaacacatgg
      481 tgatgggctt cacgaccggc acgcggagtc gcatcgaaca ggatatcgtg accaccggca
      541 tcccgttcta caccgtcggc gagaccttcg acggcgacaa gatgaagata gccgaatatg
      601 tcggcgccga caaactcgac gggcagttcg atttcccgct ctactttcag gtgcgcgaca
      661 tcctgctcca gcattcgcga gggatgaacg aactggcctc tttcgtcgcc gcgaacgaca
      721 cctactacac cgaccgctgg aacggcgcga tcatgtcgaa cttcatgggc aaccacgatg
      781 tgacccgtgc cctcaccttc gccggcggcg accacgcccg actgcggatg gcgcagacct
      841 tcctcctcac ctcgcccgcc atcccgctca tctatcaggg cgacgacatc ggcatggagg
      901 ggggcgaaga tcccgacaac cggcgcatga tggtgttcga gccatcgctt tcgaccgatc
      961 agcgggcgac gctcgctcac ctgaagaaac tcggcatggc ccgcaaggcg catccggcgc
     1021 tccgcaccgg gatccgcacg acgctcatca gtgaaacgaa cctctgggcc taccggatga
     1081 aaagcggcgc cgacgaggcc atcgtgatac tcaaccgcgg ggcacagacc cagcggcaac
     1141 tctccacaac gctcaccggc acgctcaccg acgagatatc gggcaacacc gccgccgtgt
     1201 cgggaggcaa gataacggtc accgttccgg cgatgacgag cgc
//
LOCUS       DAOQUE010000655         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_161684_length_1232_cov_3.249788, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000655 DAOQUE010000000
VERSION     DAOQUE010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161684_length_1232_cov_3.249788"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKH10_13900"
     CDS             <1..508
                     /locus_tag="PKH10_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNT29258.1"
                     /translation="LATDVEAAYEGDPAATSHGETIYCYPSIYAMTNFRIAHELHELE
                     VPLIPRIITEIAHSKTGIDLHPGAQIGEKFFMDHGTGIVVGGTSVIGRGVKIYQGVTL
                     GAKSFPLDKDGNPIKGVPRHPIVEDNVIIYSNATILGRITIGHDSVIGGNTWTMQDVP
                     PNTKVLNK"
     gene            complement(524..>1232)
                     /locus_tag="PKH10_13905"
     CDS             complement(524..>1232)
                     /locus_tag="PKH10_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29259.1"
                     /translation="TTITKLYLRGLPLLETCDLCETLESVTDELRVDCTVLSADKPRF
                     PVMSDIAYLRFGITLYGGDGYDNEAYNTVPTDALPLFGQTVTVPSAWIYAVRADDLSV
                     LAGFTPTFVKMEALTASSLDGPVISEEMSGVEITNNDLLTDISSLGALKSVAGGEFVT
                     TSQLRITGNALLADISPLMGITAVNPVVYLNITDNAALSQADAQKVADHFVNDLGLPS
                     SHVKVSGNCAACPGLIP"
BASE COUNT          273 a          383 c          354 g          222 t
ORIGIN      
        1 gctcgcgacc gatgtcgagg cggcctatga gggcgacccc gccgccacca gccacggcga
       61 gacgatatac tgctacccct ccatctacgc aatgaccaac ttccgcatcg cccacgaact
      121 gcatgaactc gaggtcccgc tcatcccgcg tatcatcacc gagatcgccc actcgaagac
      181 cggcatagac ctccatcccg gcgcacagat cggcgagaag ttcttcatgg accacggcac
      241 cggcatcgtg gtcggcggca cctccgtcat cggcaggggc gtcaagatat atcagggggt
      301 cacgctcggc gcgaaatcgt tcccgctcga caaggacggc aaccccatca agggggtccc
      361 acgccacccg atagtcgagg ataatgtcat catctactcg aacgcgacca tcctcggccg
      421 catcaccata ggacacgact cggtcatcgg cggcaacacc tggaccatgc aggatgtccc
      481 gccgaacacc aaggtgctga acaagtagtc tgcttaaaag tcgttacggt ataagtccgg
      541 gacaggcggc gcagttgccg ctcactttca catggctgct cggcagtccc agatcgttca
      601 caaagtggtc cgccaccttc tgcgcgtcgg cctgcgacag cgcggcgttg tctgtaatat
      661 tcagatagac caccgggttc actgcggtga tgcccataag cggcgatatg tcggcgagaa
      721 gggcattacc ggtgatgcgc agttggctcg tagtgacgaa ctcgccgccg gcaacgctct
      781 tcagggcgcc caacgaactt atgtcggtca gcagatcgtt gttggttatc tccaccccgg
      841 acatttcctc gctgatgacc ggaccatcaa gggaagaggc ggtcaacgcc tccatcttga
      901 cgaaagtggg cgtaaaaccg gcaagcaccg aaagatcatc ggcccggacg gcatatatcc
      961 acgccgaagg gaccgtcacc gtctgaccga agagcggcag ggcgtcggtc ggcacggtgt
     1021 tgtacgcttc gttgtcatac ccgtctccgc cgtacagcgt gatgccgaac cgaaggtagg
     1081 cgatgtcgct catgacaggg aaacggggct tgtcggccga aaggacggta cagtcgaccc
     1141 ggagttcatc ggttaccgat tcaagcgtct cacagagatc gcaggtctca agcaacggaa
     1201 ggccgcgaag atacagtttt gtgatggtcg tg
//
LOCUS       DAOQUE010000656         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_163026_length_1226_cov_3.749787, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000656 DAOQUE010000000
VERSION     DAOQUE010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163026_length_1226_cov_3.749787"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PKH10_13910"
     CDS             <1..982
                     /locus_tag="PKH10_13910"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNT29260.1"
                     /translation="AETITKNREEGLFSAVQIAVFDKGKMTVEIHDGFTSFEPKDEVT
                     RYTLFDLASLTKPLGTLVMCGELIAQKRLDPEDRATAILPELLKPIRIIDLLSHNSGL
                     PAYDAWYFKYRDIQTIEERKRAIVRYAAEFPKERPPQYSDLNYLLLGFLIEQMTGTTL
                     DRFYADTLAKIGYRGLPPIYLTDGIDLSAVAATSISPQTGILNHGNVEDENCQLIGGV
                     CGHAGLFGTAEAVGQLLSHLLKIPWYRSFIEEGTGFDRPEPPDSNFGTVATTDMRGHL
                     GYTGTAFLIDLKRGRVIVLLTNRTHPAADKPDMKERLRAFRQTIFDELLK"
BASE COUNT          272 a          402 c          330 g          222 t
ORIGIN      
        1 cgctgaaacg ataacgaaga accgcgaaga aggcctcttc tcggcggtcc agatcgcggt
       61 cttcgacaaa gggaagatga ccgtcgaaat tcacgacggg ttcacctcct tcgagccgaa
      121 ggacgaggtg acccgctata cactgttcga tctcgcttcg ctcaccaaac cgctcgggac
      181 tctggtcatg tgtggcgaac tcatcgcaca aaaaagactc gaccccgagg atagggcgac
      241 cgcgatactg cccgaactgc tgaaaccgat acggatcatc gacctgctct cacacaactc
      301 ggggcttccc gcttacgacg cgtggtactt caaatatcgc gatatccaga cgatagagga
      361 gcgcaagcga gccatcgtga ggtacgccgc cgagttcccc aaggagcgac cgccacaata
      421 cagcgacctc aactatctcc tgctcgggtt cctcattgag cagatgaccg gcacgacgct
      481 tgaccgtttc tatgccgaca cgctcgcgaa gatcggttat cgcggcctac cgccgatcta
      541 cctcaccgac ggcatcgacc tgtcagcggt cgccgcgacg agcatatcgc cgcagacggg
      601 catcctgaat cacggaaatg tcgaggatga gaactgtcaa ctcatcggcg gcgtctgcgg
      661 tcacgcggga cttttcggca ccgccgaggc ggtcgggcag ttgctcagtc acctactcaa
      721 gataccgtgg tatcgctcct tcatcgaaga gggtaccggc ttcgaccgcc ccgaaccccc
      781 cgactcgaat ttcggcaccg tcgccaccac cgacatgcgc ggtcatctcg gctacaccgg
      841 caccgccttc ttgatcgatc tcaaacgcgg ccgggtcatc gtgctcctca ccaaccgcac
      901 ccaccccgcc gccgacaaac ccgacatgaa ggaacggctc cgcgctttcc gacagacgat
      961 cttcgacgaa ctacttaaat agcctttttc tgcctccctg tcgcagggag gtggccgcag
     1021 gccggagggt gtctttaacg caacaccccc gttgccccct gcatcagggg gcctagatgg
     1081 tgtgcgaagt caacgcagag atttgaagga gaacgaagcg gttcttagtg cggaataagc
     1141 ccgagaagga tacccgccgc gatggccgac ccgataacgc cggccatcgc ggcgggtatc
     1201 cttctcgggc ttattccgca ctaaga
//
LOCUS       DAOQUE010000657         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_163462_length_1224_cov_3.842601, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000657 DAOQUE010000000
VERSION     DAOQUE010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163462_length_1224_cov_3.842601"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="PKH10_13915"
     CDS             complement(<1..1044)
                     /locus_tag="PKH10_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29261.1"
                     /translation="MDKRKKLLKLFRDIRSFCSDHDIAANPYTFLLNRYYFTTLGKRI
                     SPSLLTGISADDFLKINRRFSDRLKHELVSSWSVSYILSLPHPAPMETSPRLSSSQHD
                     ILFLSDAIYLPRHLEKGHRLHFASHDEGAFLSPFTEVFAYPFSKDKPVEISLQSTIFD
                     LFSGEQSTKVKPYQFHLVLDEAITSDFPPEQFFRNIALLLHEEGSYLLHLKKNFLMSP
                     VTKKEKRFLYDIFSLREINKDHQKLVLDLGRKRTPEPERSIAISDRATGSFFTVPNDF
                     ITFNNLLTFNDNLAYEEILLLQKIEKQAEGTCADHFDFFIGMFPARGTQPPVEALRRT
                     PQTKPLIRGKEIVP"
BASE COUNT          286 a          277 c          392 g          269 t
ORIGIN      
        1 ggggacgatc tccttgccgc ggatgagcgg tttggtctgc ggggtacggc gcagggcctc
       61 gacaggaggc tgtgtgccgc gggcggggaa catcccgatg aagaaatcga aatggtcggc
      121 gcaggtccct tcggcctgtt tctctatctt ctggaggagc aggatctctt cgtaggcgag
      181 attgtcgttg aaggtgagca gattattgaa ggtgatgaag tcgttcggca cggtaaagaa
      241 cgatccggtc gcgcggtcgc tgatggcgat ggaccgttcg ggctccggcg tccgtttccg
      301 cccgagatcg agcaccagtt tttgatggtc cttgtttatc tcgcgcagag agaagatatc
      361 gtaaaggaac cgtttttcct tcttggtcac cggcgacatc aggaaattct tcttgaggtg
      421 gagcaaatag gacccctcct catgcaaaag aagagcgatg ttgcggaaga attgttccgg
      481 cgggaagtcg ctggttatcg cttcgtctag gaccagatgg aactgatagg gtttgacctt
      541 ggtgctctgc tcacccgaga agaggtcgaa gatcgtcgac tggagcgata tctccaccgg
      601 cttgtccttg gaaaagggat aggcgaacac ctccgtgaac ggcgatagga acgccccctc
      661 atcgtgggag gcgaaatgga ggcgatgtcc cttttctagg tgacgcggca gatagatcgc
      721 gtctgaaagg aacaggatat cgtgttggga ggaggaaaga cgcggagagg tctccatcgg
      781 cgccggatgg gggagcgaga ggatgtaact gaccgaccac gaggagacca gttcgtgttt
      841 caggcgatcg ctgaagcggc ggtttatctt cagaaaatca tcagccgata tcccggtgag
      901 gagcgagggg gatatccgtt tgcccagcgt ggtgaagtag tagcgattca gcaggaaggt
      961 ataggggttc gccgcgatat catgatcgct acagaaggat cggatatccc ggaacagctt
     1021 aagaagcttt ttcctcttat ccatgaaaaa tgccccgata catgcttcga taccatatag
     1081 ccatcaaggt caaatagcaa aaaaaatctt gatgcgaaaa aaagggggcc gcgcgggccc
     1141 cctcgaagta gtggtatgag agttagagat cagttgtcgg gaagcaggtc atcggtggcg
     1201 acatcatcgg tcgtttcgtc accg
//
LOCUS       DAOQUE010000658         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_167151_length_1208_cov_4.016479, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000658 DAOQUE010000000
VERSION     DAOQUE010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167151_length_1208_cov_4.016479"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="PKH10_13920"
     CDS             <1..1053
                     /locus_tag="PKH10_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29262.1"
                     /translation="WVVYEATTETGTYTLIAQKTTTATATTAAWYSSGGMTRTDVGHV
                     GETITLTSGRYYAIGLAWNESLTSYYWSPGFLQPFETEYTMFGATLGGTANDATYPPL
                     ASFSGGPANDSTYWMRVHVLNTDADLCVCQNECDAAGIQQCNVNAVEECVADIYGCLS
                     WSFSEDCVADYTPDRVCTESLDTATCEEEVAEVADTIGSSSTLYTGGTSRMRGNYYSC
                     DTTRTLTEIEMYLDPASTATVNFVVYSSTTLGGTYYPVATVAQSTSTGAGFKSSGTMS
                     VALQAGTYYFIGAWWGSSVTVGYAANTSATYYDTAFGSWDSGMTLESVTSAPASLDYD
                     STYYSGSAYYQRLTTQ"
BASE COUNT          242 a          354 c          366 g          246 t
ORIGIN      
        1 tgggtcgtgt acgaggcgac caccgagacc ggtacctaca cccttatcgc gcagaagacg
       61 acgaccgcca ccgccacgac cgccgcatgg tattcttcgg gcgggatgac ccgtaccgat
      121 gtcggccatg tcggcgaaac gataacgctt accagcgggc gctactacgc catcggtctc
      181 gcctggaacg agagtctgac ctcctactat tggtcgccgg gcttcctgca gcccttcgag
      241 accgaataca cgatgttcgg tgcgacgctc ggcggcacgg cgaacgatgc tacctatccg
      301 ccgcttgcca gtttctcggg cggtcccgcc aacgacagca cctattggat gcgggtccat
      361 gtcctcaata ccgatgccga cctctgcgtc tgccagaacg agtgcgacgc cgcgggcatc
      421 cagcagtgca atgtgaacgc cgtcgaggaa tgcgtcgccg atatctacgg ctgtctttcg
      481 tggtccttca gcgaggattg tgtcgccgac tatacgccgg accgtgtctg caccgaatcg
      541 ctggataccg ccacctgcga ggaggaggtg gccgaggtcg ccgatacgat cggtagcagt
      601 agtaccctct acacgggggg gacaagccgg atgcgcggta actactactc ctgcgatacc
      661 acgcgaacct tgacggagat agagatgtat ctcgatcccg cgagcaccgc cacggtcaat
      721 ttcgtggtgt actcgtcgac cacgctcggc gggacctatt atccggtggc aacggtggcg
      781 caaagcactt caacgggagc cggtttcaag agttccggca cgatgtcggt cgcgctacaa
      841 gcgggtactt attatttcat cggtgcttgg tggggctctt cggtcacggt cggatatgct
      901 gcgaacacct cggcgaccta ctacgatacc gcgttcggct catgggatag tgggatgacg
      961 ctcgaatcgg tgaccagcgc gcccgcctct ctggattacg attcgaccta ctactcgggt
     1021 agcgcctatt atcagcggct gacgacgcag taaaccgacc ataaagataa ggaggaatag
     1081 ggacatgaga tctttttttg ccatcgtggc gttggtcaca gtgctgttgc tgttcggttg
     1141 cgagagcggg accaagaaat tcgtgccgaa ggacgatgtg gcgctgggtg acagtgacga
     1201 actgctcg
//
LOCUS       DAOQUE010000659         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_173942_length_1180_cov_3.857778, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000659 DAOQUE010000000
VERSION     DAOQUE010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173942_length_1180_cov_3.857778"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="ribA"
                     /locus_tag="PKH10_13925"
     CDS             <1..786
                     /gene="ribA"
                     /locus_tag="PKH10_13925"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNT29263.1"
                     /translation="DLARIARLKPAAVICEVMDEDGTMARRPQLEVFSKEFDIPIVSV
                     ADIIAYRLASESLIDLVAESVLPTRAGGDFAIRVYRNRIDGAEHIALIKGDIAKGAPA
                     LVRVHSECLTGDALGSIRCDCGSQLENAMAMIRDAGRGVVLYLRQEGRGIGLGNKIKA
                     YHLQDEGMDTVEANIALGFPPDLRDFGIGAQILKAIGLRRIRLITNNPKKFISLSGYG
                     LEIVEQVSSRAVVTPQNERYLQTKQKKMGHTIFTDQPQKGEKQ"
     gene            783..>1180
                     /gene="ribH"
                     /locus_tag="PKH10_13930"
     CDS             783..>1180
                     /gene="ribH"
                     /locus_tag="PKH10_13930"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNT29264.1"
                     /translation="MKTNEFHGELTGKGKKFAIVLSRFNSFLSESLLKGALDCLDRHE
                     ATDVDIVRVPGSFEVPFAAKRLAASKKKYDAIICLGALIQGETPHFQFIAAEAAKGIA
                     QVALETGVYCAFGIITADTLEQAIERAGTKG"
BASE COUNT          269 a          339 c          372 g          200 t
ORIGIN      
        1 gacctcgccc ggatagcgcg actcaagccg gccgccgtca tctgcgaggt gatggacgaa
       61 gacggcacga tggcccgccg tccgcaactt gaagtgttct caaaagaatt cgatatcccc
      121 atcgtgtcgg tagccgacat catcgcctac cggctcgcca gcgaatcgct catagatctc
      181 gtcgccgagt cggtcctgcc gacgcgcgcc ggcggcgatt tcgcgatacg ggtctaccgc
      241 aaccggatag acggggccga acatatcgct ctcataaaag gcgacatagc gaaaggggcc
      301 ccggccctcg tgcgggtcca cagtgaatgc ctgaccggcg acgcgctcgg gtcgatccgg
      361 tgcgactgcg ggtctcaact cgagaatgcg atggcgatga tacgggatgc ggggcgcggg
      421 gtcgtcctct atctgcggca ggaggggcgc ggtatcggcc tcggcaacaa gataaaggca
      481 taccacctgc aggacgaggg tatggacacc gtggaggcga acatcgcgct cggtttcccg
      541 ccggatctgc gcgatttcgg catcggggcg cagatactca aggctatcgg cctgcgccgg
      601 atacggctca taacgaataa tccaaagaaa ttcataagct taagcggcta cgggctagag
      661 atagtcgaac aggtgtcgag ccgtgcggtg gtcacgccgc agaacgaacg gtacctccag
      721 acgaaacaga agaagatggg gcacactata tttaccgatc aaccgcaaaa aggggaaaaa
      781 caatgaagac gaacgaattc catggcgaac tgaccggaaa gggaaagaaa ttcgccatcg
      841 tgctgtcacg attcaacagt ttcctgagtg aatcgctcct caagggggcg cttgactgcc
      901 ttgaccggca cgaggcgacc gatgtcgaca tcgtccgggt cccgggctca ttcgaggtgc
      961 cgttcgcggc caaacggctc gcggcatcga agaagaagta cgatgccatc atctgcctcg
     1021 gagcgctcat tcagggggag accccgcatt ttcagttcat cgccgccgag gcggccaagg
     1081 ggatcgcgca ggtcgctctg gaaacgggtg tgtactgcgc cttcggtatc atcaccgccg
     1141 atacgcttga gcaggcgata gagcgggcgg gcaccaaggg
//
LOCUS       DAOQUE010000660         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_174913_length_1176_cov_3.566459, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000660 DAOQUE010000000
VERSION     DAOQUE010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174913_length_1176_cov_3.566459"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PKH10_13935"
     CDS             complement(<1..910)
                     /locus_tag="PKH10_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29265.1"
                     /translation="MPTFTADRFLRIASGLLLASFIVFALIVVARELARSRDPLIRLA
                     AEQCVTLAAAGREAPPGAEFLPAVPASEQDLMKSGASCIFFDDTVRDASPLTAAVFAR
                     KPIAGWDHLFLGPRGALIIRDERDFAAGAIALRDCLRNDRCDTDPTRDPVVSLTVSLL
                     KRSGQQRPLTVSFYDETFGAILAKLPGLLREMANAKKLDTAEMTLETAFHRHYARIME
                     PDEKKIAALAMKERDGLMLASRGARIRLLPWEYDKNPLRRLSAKGTQYGLEKEEYKKD
                     VASVFIFSTVRHRETDGAMMPVETTPK"
     gene            complement(925..>1176)
                     /locus_tag="PKH10_13940"
     CDS             complement(925..>1176)
                     /locus_tag="PKH10_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29266.1"
                     /translation="LPAVPGYLPAPVMVDDKAVIAMVAKHTPADMAKFTTEKETIRRS
                     LQGGKSREIISSYTKALLEKAAVEYNHNWISAFGTGEGQ"
BASE COUNT          191 a          348 c          408 g          229 t
ORIGIN      
        1 ctttcggggt ggtctcgacg ggcatcatag ccccgtccgt ttcgcgatgc cggacggtcg
       61 agaatatgaa taccgacgcg acatcttttt tgtactcctc cttttcgagc ccgtactgcg
      121 ttcctttcgc ggaaaggcgg cggagcgggt tcttgtcgta ttcccacggc aggaggcgta
      181 tccgggcgcc gcgcgaggcg agcatgagcc cgtcgcgctc cttcatggca agcgccgcta
      241 tctttttctc gtcgggctcc atgatgcggg cgtagtgccg gtggaacgcc gtttccaatg
      301 tcatctcggc ggtatcgagt tttttcgcgt tcgccatctc gcgcagaagc cccggcagtt
      361 tcgcgagtat cgccccgaac gtctcgtcat agaaggatac ggtgagcggg cgctgctggc
      421 cggagcgttt cagaagcgag acggtcagcg atacgacggg gtcgcgtgtc gggtcggtgt
      481 cgcaacggtc gttgcgcagg cagtcgcgca gagcgatcgc cccggcggcg aagtcccgtt
      541 cgtcgcggat gatgagcgca ccgcgagggc cgaggaaaag gtggtcccac cccgcgatcg
      601 gttttcgcgc gaacaccgcg gcggtgagcg gcgatgcgtc gcgcaccgta tcgtcgaaaa
      661 agatgcagga ggcgccgctt ttcatgaggt cctgttcgct tgcggggaca gcgggaagga
      721 attcggcgcc ggggggcgct tcgcggccgg cggcggcgag cgtcacgcac tgttcggcgg
      781 caagccggat gagcgggtca cgcgaccggg ccagttcacg cgccacgacg atgagggcga
      841 atacgataaa ggaggcgagc aacaacccgc ttgctatgcg aaggaatcta tcggcggtga
      901 aggtcggcat gccgtgccgc ccggtcactg accttcgccg gtgccgaacg cggagatcca
      961 gttgtggttg tattcgacgg ccgctttctc aagcaaggcc ttggtgtagg aagagatgat
     1021 ctcgcgcgat ttaccgccct gcaggctccg ccggatggtc tctttctcgg tggtgaactt
     1081 ggccatgtcg gccggcgtgt gcttggccac catcgcgatg accgccttgt cgtccaccat
     1141 gaccggcgcc gggaggtacc ccggaacggc gggcag
//
LOCUS       DAOQUE010000661         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_175427_length_1174_cov_3.075067, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000661 DAOQUE010000000
VERSION     DAOQUE010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175427_length_1174_cov_3.075067"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PKH10_13945"
     CDS             complement(<1..364)
                     /locus_tag="PKH10_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29267.1"
                     /translation="MTRWFTLCAAVFFALLFVACDDNDKTTTDLDTVADTDAATDGTV
                     TDDVITDDILTDTEETPDDDAFVIGDCTKVMVQDIAIVEGFYEGAFSMDLGEAALPDL
                     FSIQFYVDATTVKTALTVG"
     gene            complement(485..>1174)
                     /locus_tag="PKH10_13950"
     CDS             complement(485..>1174)
                     /locus_tag="PKH10_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium symporter"
                     /protein_id="HNT29268.1"
                     /translation="VIPAAFVFLGAAGTMEVAKSGLFNIGFVTMPLIFGKIPLGALFA
                     GVWFLLLFIAGITSSVSMVQPAMSFLQDELKVDRKKATGILGIIAFLACQPAIFFLGH
                     GFVDELDFWGGTVFLVLFATVETVIFGWVIGIDKAWEEMHQGADIRIPSAYKFIIKYV
                     TPLYLFAILGAWSYQQFIPTILMEGIAEVDKPYIWGARLLVFGGLAAVIALTVYAARR
                     MKNDGAAGGAA"
BASE COUNT          268 a          346 c          344 g          216 t
ORIGIN      
        1 agcccacggt cagggccgtc ttgacggtgg tggcatcgac atagaactgg atcgagaaga
       61 gatcgggcag agcggcctcg ccgagatcca tcgagaaggc gccttcatag aaaccttcta
      121 cgatggcgat atcctgcacc atcaccttgg tacagtcgcc gatgacgaag gcatcgtcat
      181 ccggcgtctc ttcggtatcg gtcagaatgt cgtcggtgat gacatcgtcg gtaacggtcc
      241 cgtcggtcgc cgcgtcggtg tcggcgacgg tatcgagatc ggtcgttgtc ttgtcattgt
      301 cgtcgcaggc gacgaacaac agcgcgaaaa acaccgccgc gcacagggtg aaccatctgg
      361 tcatgtatgc ctccttgata agggttgggg acctatttct tccggaacat ccggtagtac
      421 gaaaaaacaa ggagcgcaac gatgctcccc cacgacagcg ccatgaaaag tatcgcctcg
      481 atattcatgc cgcacctcca gccgccccgt cgttcttcat tcgtctggcc gcgtaaacgg
      541 tgagcgcgat gaccgcggcg agtccaccga aaacgagcag acgcgctccc cagatgtagg
      601 gtttgtcgac ctccgcgatc ccttccataa gtatcgtcgg gatgaactgc tggtaactcc
      661 acgcaccgag tatcgcgaag aggtagagcg gcgtcacata tttgatgatg aacttgtagg
      721 cggacgggat acggatatcg gccccctgat gcatctcctc ccatgccttg tcgataccga
      781 tgacccagcc gaagatgacg gtctcgaccg tggcgaacag gacgaggaac actgtgccgc
      841 cccagaaatc gagttcgtcc acaaagccgt gaccgaggaa gaatatcgcc ggctggcagg
      901 caaggaacgc gatgatgccg aggataccgg tcgccttttt gcggtcgacc ttgagttcgt
      961 cctgcaggaa cgacatggcg ggctgcacca tcgagaccga cgaggtgatg cccgcgatga
     1021 acagcagcag gaaccacacc cccgcgaaca gcgccccgag cggtatctta ccgaatatga
     1081 gcggcatcgt cacgaagccg atattgaaga gtccgctctt ggcgacctcc atcgtacccg
     1141 ccgcgccgag aaagacgaag gcggccggga tgac
//
LOCUS       DAOQUE010000662         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_175690_length_1173_cov_2.961538, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000662 DAOQUE010000000
VERSION     DAOQUE010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175690_length_1173_cov_2.961538"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="PKH10_13955"
     CDS             complement(<1..1014)
                     /locus_tag="PKH10_13955"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT29269.1"
                     /translation="MNMPDRKSVPVTRPFLPPLAGFIPRLERIWESGWVTNGGPQHQE
                     LEQALCRYLDVPFVSLFNNGTNALITALQALDLPEGSEVITTPFSFVATAHTVRWNRC
                     MPVFADVDPVHGNIDPAVVERAITPATRALLPVHVYGNPCDTAAIADIAKRHKLQVLY
                     DAAHLFGVTRPARPPLRDGDLSVLSFHATKVFGTFEGGAIVSHTEEMKHKIDSLRNFG
                     FTGETTADHVGMNGKMNEVQAAFGLLSLAHIDAAIAKRRAVAERYIAALSGIHGIGVI
                     RPADGVGPAWPYFPITVDATRYGRSRDELFEEFKRRDILPRRYFYPLISAFAPYRDLP
                     SAVP"
BASE COUNT          251 a          321 c          396 g          205 t
ORIGIN      
        1 cggaacggcc gacgggagat cgcggtaggg ggcgaaggca ctgatgagcg gatagaaata
       61 gcggcgcggc aggatatcgc gccgtttgaa ttcttcgaac aactcgtcgc gggaccgacc
      121 gtagcgtgtc gcatcgaccg ttatcggaaa gtagggccac gccggtccga ccccgtcggc
      181 gggacgaatg acgccgatac cgtggatacc cgaaagtgcg gcgatgtacc gttcagcgac
      241 cgcgcggcgt ttcgctatcg ccgcatcgat atgtgcaagc gacaggagac cgaaggccgc
      301 ctgcacctcg ttcatcttgc cgttcatgcc gacatggtcg gcggtcgttt cgccggtgaa
      361 gccgaaattc cgcaacgaat ctatcttgtg cttcatctct tcggtgtgcg acacgatcgc
      421 ccccccctcg aaggtgccga aaaccttcgt cgcatggaaa ctgagcaccg acaggtcacc
      481 gtcgcggagc gggggacggg cgggacgcgt cacgccgaaa agatgcgccg catcatagag
      541 cacttggagt ttatgccgtt tcgcgatatc ggcgatggct gcggtatcgc acggattgcc
      601 gtagacatgg accggcagga gggcgcgggt cgcgggggtg atggcccgct ccactacggc
      661 ggggtcgatg ttgccgtgga ccggatcgac atcggcgaac accggcatgc aacggttcca
      721 gcggaccgtg tgggccgtcg cgacaaaact gaagggggtg gttatcacct ccgatccttc
      781 gggcagatcg agtgcctgca gggcggtgat gagggcattg gtgccgttgt tgaagagcga
      841 cacgaagggg acatccagat accgacagag tgcctgttcc agttcctgat gctgaggacc
      901 gccgttggtg acccagccgc tttcccagat ccgttcaaga cgagggatga atccggcgag
      961 tggcggcagg aacggacggg tgaccggcac gctcttgcgg tcgggcatgt tcatcgaggg
     1021 aagatcctcc gtatctgtcc ggcgacgata agacgaagtt cagtatacgc agacaatttg
     1081 aaaaagcgac ataaaagcag atatgctgtc ccgcccgtga cgagctgcga cagaagcaga
     1141 agcacagggg aagggatagg aaagagcgcg gtc
//
LOCUS       DAOQUE010000663         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_176876_length_1168_cov_3.832884, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000663 DAOQUE010000000
VERSION     DAOQUE010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176876_length_1168_cov_3.832884"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PKH10_13960"
     CDS             complement(<1..793)
                     /locus_tag="PKH10_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT29270.1"
                     /translation="MRIVVTLTLFTLLVGCATVAPVAEPEKKQPVAATGERTVQKIES
                     RVALYVTAKGAELREITPEEKEDHDALFTMANKFFDLNRYEDAQKLYDRIVQKNPDHS
                     MAPFCHYNLGLINMKTLNWPVSAAHFQTAYRTLAKKDDKKDALILYLEALKKAASWET
                     LLSEANTALNDNPYQLDFGDETTQEISLRSAEAMVMMGKLEEGRRLADYWITEIRKGL
                     SREEQLFVPNLALAFYVMGKSQVYEFGSFKLTEAMETLVRKCQKII"
     gene            complement(858..>1168)
                     /locus_tag="PKH10_13965"
     CDS             complement(858..>1168)
                     /locus_tag="PKH10_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29271.1"
                     /translation="GINDLALSSDEKMAAAVTEDGATRLFSLPSGRPLLYLDTGDGGS
                     AVFARGVGTVFASDGTEIKKFPLEESYLDNDPGLLFEDSQKRSGLRLNGTQVEPVEEM
                     "
BASE COUNT          238 a          335 c          310 g          285 t
ORIGIN      
        1 cgattatctt ctggcatttg cgcaccagcg tctccatcgc ctcggtcagt ttgaacgaac
       61 cgaattcgta cacctgactt ttacccatca catagaatgc aagcgcaagg ttggggacga
      121 acagttgttc ctcgcgcgac aggcctttcc gtatctcggt tatccagtaa tcggccaacc
      181 ggcgaccctc ttccagtttg cccatcatga ccatcgcctc ggccgagcgg agagatatct
      241 cctgcgtcgt ctcgtcgcca aaatcaagtt ggtaggggtt atcgttgagc gcggtgttcg
      301 cttccgaaag aagcgtttcc cacgaggccg ctttcttgag cgcttcaaga taaaggatca
      361 aggcatcttt cttgtcatcc ttcttggcga gggtgcggta tgccgtctgg aaatgggctg
      421 cggacaccgg ccagttgagc gttttcatgt tgatgagccc cagattataa tgacagaacg
      481 gggccatcga gtggtcaggg ttcttctgaa ctatgcgatc gtaaagtttt tgggcatcct
      541 cgtaacggtt aagatcgaaa aatttgttcg ccatggtgaa cagggcgtca tgatcctcct
      601 tttcttccgg cgttatctca cgcaactcgg ctcccttggc cgtcacatac agcgcgacgc
      661 gggactcgat cttctggacc gtgcgctccc ctgtagcggc gaccggttgt ttcttttccg
      721 gttcagcgac gggtgcgacc gtggcacagc cgacaagcaa agtgaacaaa gtcaaagtca
      781 ctacgatcct catgagatcc tcctttctgg gattaatgat gtggatacct atcgtggttc
      841 tcttttcata tgttcactca catctcctcg accggctcga cctgcgtgcc gttcaagcgc
      901 aggcccgatc gtttttgaga atcttcgaag agaagtcccg gatcgttatc gagataggat
      961 tcctccagcg ggaatttctt tatctcggtg ccgtcggagg cgaacaccgt gccaacacca
     1021 cgggcaaaga ccgccgagcc gccatcgccg gtatcgaggt acagcagcgg tcggccgctg
     1081 ggaagcgaga acaggcgcgt cgccccatcc tccgtgaccg ccgccgccat cttctcgtcg
     1141 gacgaaagcg caagatcgtt gatcccgc
//
LOCUS       DAOQUE010000664         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_177154_length_1167_cov_3.971223, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000664 DAOQUE010000000
VERSION     DAOQUE010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177154_length_1167_cov_3.971223"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PKH10_13970"
     CDS             complement(<1..824)
                     /locus_tag="PKH10_13970"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNT29272.1"
                     /translation="MKNIVGSIILCGLLAAAVSCSKPAGVVGENEIPPYAQKVSALLD
                     DGKIELYAFYTEPLKPQHGQSFKVVSFWRFKEQLPEGYKLFFHFEDPSGEQIFVYDHP
                     LADGRFKELPLKGLIRDDASIKELPVWFDSDRIALRAGFFKDDARLIPDPKFNDGKNR
                     LKLPEIEISKPKLVKKQLKVFAVAGESRKQIKIDGKLKESYWQNAQTGGKFWLTSGEN
                     TAQKQTNVLTTMDEKYLYFAFDCEDDDVHATLKDNDDKIYDHDDVVEIFIDANGDR"
     gene            976..>1167
                     /locus_tag="PKH10_13975"
     CDS             976..>1167
                     /locus_tag="PKH10_13975"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNT29273.1"
                     /translation="MARRTTDIAKLLTDHLDTMFGYALFITGDRERAMDLLQDTAVGL
                     IAKKDLPYEERDAFRAWLFR"
BASE COUNT          210 a          341 c          343 g          273 t
ORIGIN      
        1 ttccggtcgc cgttcgcgtc tatgaatatc tcgaccacat cgtcgtgatc gtatatcttg
       61 tcgtcgttgt ccttgagcgt cgcatggaca tcgtcatcct cgcagtcgaa ggcgaaatag
      121 aggtatttct cgtccatcgt ggtcaggaca ttggtctgct tctgcgcggt gttctcgccg
      181 ctcgtgagcc agaacttgcc gcccgtctgg gcgttctgcc agtaactctc cttcagtttg
      241 ccgtctatct ttatctgttt acgcgattcg cccgccaccg cgaagacctt caactgcttc
      301 ttcaccagtt tcggcttgga tatctctatc tcgggcagtt tcagccggtt cttgccgtcg
      361 ttgaacttcg ggtcggggat aagccgggca tcatctttga agaacccggc gcggagcgcg
      421 atgcggtcgc tgtcgaacca gaccggcagt tccttgatcg atgcgtcgtc acgtatgagc
      481 cccttgagcg gcagttcctt gaaccggccg tcggccagcg ggtggtcgta gacgaatatc
      541 tgttcgccgc tcggatcctc gaagtggaag aagagtttat agccctcggg gagctgttcc
      601 ttgaagcgcc agaacgagac caccttgaaa ctctggccgt gctggggctt gagcggctcg
      661 gtgtagaagg cgtacagttc gatcttgccg tcgtcgagga gcgcgctcac cttctgggcg
      721 tacggcggta tctcgttctc gccgaccacc ccggccggtt tcgagcagga gacggcggcg
      781 gcaaggaggc cgcacaggat aatggacccg acgatgtttt tcatgttccc tccgtgttct
      841 tgacaagacc tcgggaccac tctagtcgct cctgcgccgt ttctcaagaa aaaaagagag
      901 gggaatattt ctcaagcggc cgctgttccg gtatagtgcg agccgatatg acggtacgga
      961 aacggagggt ctgacttggc gcggcggacg accgatatcg cgaaactgct caccgaccat
     1021 ctcgacacga tgttcgggta cgccctgttc atcaccggcg accgcgaacg ggcgatggat
     1081 ctcctgcagg acacggcggt cggtctgatc gccaaaaaag acctgcccta cgaggagcgc
     1141 gacgccttcc gcgcatggct gttccgc
//
LOCUS       DAOQUE010000665         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_177411_length_1166_cov_3.934293, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000665 DAOQUE010000000
VERSION     DAOQUE010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177411_length_1166_cov_3.934293"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKH10_13980"
     CDS             complement(<1..284)
                     /locus_tag="PKH10_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29274.1"
                     /translation="MKHVVGCLGAVAFFLLVASCGDESAQKTSAGGTLSLWEEQRDEP
                     LYGMEEATQEAWLVTVQKRLATGEYRISASDNGFRATNRAQGLRFLTGPEG"
     gene            complement(379..>1166)
                     /locus_tag="PKH10_13985"
     CDS             complement(379..>1166)
                     /locus_tag="PKH10_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNT29275.1"
                     /translation="PWVQRITMFGGNPTAENIAAGNDTADETICQWMNSSGHRTNICS
                     PSNTSLGTGYHQGQNCFYRYWTQDFGGGAQAERLYPGALFEDGAILKAGLIVRSPSPV
                     KTAAVTVGTVETALANTFGTDLRGAWTATVSAANCAAYFFTASFDDGKSERFPPAGYL
                     FYSETPEQCETPDGDPDIDTVKPDDTVPDDAASDDEGSDTTEGPDDETPDEGSSDHTS
                     PFDFNPSKDDAGPANDDGPVDVDLVWPDTDQKNDAGCGCSLVF"
BASE COUNT          212 a          375 c          362 g          217 t
ORIGIN      
        1 ccctccggcc cggtcaggaa acgaagaccc tgcgcgcggt tcgttgcgcg gaatccatta
       61 tccgacgccg atatgcgata ctcgcccgtt gcgaggcgct tctgcaccgt cacgagccac
      121 gcttcctgcg tcgcctcctc catcccgtac agcggctcgt cacgctgttc ttcccacaga
      181 gagagcgtcc cgcccgcact ggtcttctgc gcggactcgt cgccacagga ggcaacaaga
      241 aggaaaaagg ccacggcccc aaggcatcca acaacatgtt tcatcaggct ctccccagat
      301 agtgataaaa acattcgaca gcacggcatg ctacccgaat gtgaaaaaaa tctcaagaaa
      361 agtgggcgaa cggatcgttc aaaaaaccag cgaacacccg cacccggcat cgttcttttg
      421 gtcggtgtcg ggccagacaa ggtccacatc gaccggccca tcgtcgttcg cgggaccggc
      481 atcgtccttt gacgggttga aatcgaaggg agaagtgtga tcggacgacc cttcgtcggg
      541 cgtttcgtcg tcaggcccct ccgttgtgtc gctcccctcg tcgtcggacg ccgcatcgtc
      601 gggcaccgtg tcatccggtt tcacggtgtc gatatcggga tcgccgtcgg gcgtctcaca
      661 ctgttcgggc gtttcgctgt agaacagata cccggcgggc gggaaccgct cgctcttgcc
      721 gtcgtcgaac gaggcggtga agaaataggc ggcgcagttc gccgccgaga cggtcgcggt
      781 ccacgcgccg cgcaggtcgg tgccgaaggt gttggcgagc gcggtctcga ccgtccccac
      841 cgtgacggcg gccgtcttga ccggggacgg cgacctcacg atgagccccg ccttgaggat
      901 cgcgccatcc tcgaacagcg cgccggggta aagccgttcc gcctgcgcgc cgccgccgaa
      961 gtcctgtgtc cagtagcggt aaaaacagtt ctggccctga tggtaaccgg tgccgagcga
     1021 ggtgttcgag ggcgagcaga tgttcgtgcg gtgacccgac gaattcatcc actggcagat
     1081 cgtttcgtcg gcggtgtcgt tgccggcggc gatgttctcg gcggtcgggt tgccgccgaa
     1141 catcgttatc cgctggaccc acgggg
//
LOCUS       DAOQUE010000666         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_181670_length_1150_cov_2.968037, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000666 DAOQUE010000000
VERSION     DAOQUE010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181670_length_1150_cov_2.968037"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1150
                     /locus_tag="PKH10_13990"
     CDS             <1..>1150
                     /locus_tag="PKH10_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNT29276.1"
                     /translation="ADMDRDAIRDLFRPRPSSLTLCHDIMRFRVREILLVASVYDAYI
                     IEREGQLMEQIYGEYYQLNLSTAPRVTAVHSSDEAMSALRDRRYDLVIIMTGLDRRMP
                     LDLSTRIRDAVPELPVLLLFNNNSDIGMYAYTAAELRHIDRVFVWNGDPAIFLAMIKS
                     VEDVRNADEDTRIAATRVILLIEDSVRYYSRYLPGLYRVLMQDMQKLIAGEDIDARFK
                     ILRMRARPKVLIATSWEEGSALYDRYRDFILCVISDVEFPRSGKLDPVAGVELAKLLR
                     ARDDVPVLLQSTDETNAEKARDAGTRFVNKNSKTLVKELHRFFEEELGFGNFVFRDAQ
                     GRKLIEARYMAEFEALIATVPDESILYHAARHNFSQWFMAKGEVATAIA"
BASE COUNT          225 a          389 c          329 g          207 t
ORIGIN      
        1 gcgctgacat ggaccgtgac gccatccgcg acctgttccg cccgcgtccg tcgagtctca
       61 cgctctgcca cgacatcatg cgcttccggg tccgcgagat actgctcgtc gcgtcggtgt
      121 acgatgccta catcatcgag cgcgaaggcc aactgatgga gcagatatac ggcgaatact
      181 accaactcaa cctctcgacc gcgccgcgtg tcacggcggt ccactcctcc gacgaggcga
      241 tgtcggccct gcgcgaccgt cgctacgacc tggtcatcat catgacggga ctcgaccgcc
      301 ggatgccgct cgacctctcg acgcgtatcc gtgacgcggt gcccgaactg ccggtgctcc
      361 tgctgttcaa caacaacagc gacatcggca tgtacgccta taccgccgcc gaactgcgcc
      421 acatagaccg cgtcttcgtc tggaacggcg accccgccat cttcctcgcc atgatcaagt
      481 cggtcgagga tgtccgcaac gccgacgagg acacccgcat cgcggcgacc cgcgtgatcc
      541 tgctcatcga ggattcggtc cgctactatt ctcgctacct gccgggactt taccgcgtgc
      601 tcatgcagga tatgcagaaa ctcatcgcgg gcgaggacat cgacgcgcgg ttcaagatcc
      661 tgcggatgcg ggcccgcccc aaggtgctta ttgccacctc gtgggaggag gggagcgccc
      721 tctacgaccg ctaccgcgat ttcatcctct gcgtcatatc ggatgtggaa ttcccgcgca
      781 gcggcaaatt ggacccggtc gccggcgtag agttggcgaa actgcttcgc gcacgggacg
      841 atgtgccggt actcctgcag tcgaccgatg agacgaacgc ggaaaaggcc cgtgacgccg
      901 ggacccgctt cgtcaacaag aactccaaga cgctcgtgaa ggaactgcac cgcttcttcg
      961 aggaggaact cggcttcggc aatttcgtat tccgcgatgc acaaggccgc aaactcatcg
     1021 aagcgcgcta catggccgag ttcgaggcac tcatcgcgac ggtccccgac gagtcgatac
     1081 tctaccacgc ggcgcgacac aatttctcgc agtggttcat ggccaaaggc gaagtggcga
     1141 ccgccatcgc
//
LOCUS       DAOQUE010000667         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_182483_length_1147_cov_2.881868, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000667 DAOQUE010000000
VERSION     DAOQUE010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182483_length_1147_cov_2.881868"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKH10_13995"
     CDS             complement(<1..646)
                     /locus_tag="PKH10_13995"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HNT29277.1"
                     /translation="MILIDISLPLLFMALLIRFRVRLEIVLPAGALVLALLFDMGPLD
                     AALVYGELFGSMQFLALFCVIFFVMLLGELMERGKMLSQMSSIFLSLFRDNRLAAAAS
                     TAFIGLLPMPGGSLLSAPFVEKLVPSEKSEHKLVLNYWYRHIWEYFYPLYSGMAWIIT
                     VMKAPPLSILLYLGPLSLIMIAAGLPFIRHAPRETPPGLDGGARPHRLLLIGWPI"
     gene            complement(643..>1147)
                     /locus_tag="PKH10_14000"
     CDS             complement(643..>1147)
                     /locus_tag="PKH10_14000"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNT29278.1"
                     /translation="LLDLCAGGGGKTLALAAQRSDLRITASDIRTHRSDEITERATRA
                     GTPVTTIAPEKLADTRFDAVLVDAPCSGAGVLRRNPEDRWRVGEKEIATLLESQRTCL
                     GTALRHLAPGGTILYLTCSYLPVENEENIAKFISAHHDVAADGPGMLTVPGGTADLMF
                     AMFLRRR"
BASE COUNT          234 a          336 c          392 g          185 t
ORIGIN      
        1 gtatgggcca accgatgagg agcaggcggt gagggcgtgc gccgccgtcg agcccgggcg
       61 gcgtctcgcg gggggcatgg cggatgaagg ggagtccggc ggcgatcatg atgagcgaga
      121 gcgggccgag gtagagcagt atcgagagcg gtggtgcctt catcaccgtg atgatccagg
      181 ccatccccga atagagcgga tagaaatact cccagatatg gcgataccag tagttgagca
      241 ccagtttatg ctcgcttttc tcggacggga ccagtttctc gacgaaaggc gccgataaca
      301 gcgatccgcc cggcatcggc aggagcccga tgaaggcggt cgaggcggcg gcggcgagcc
      361 ggttgtcacg gaagagcgaa aggaaaatgg acgacatctg cgagagcatc tttccgcgct
      421 ccatcagttc gccgaggagc atcacgaaga aaatgacgca gaacaaggcg aggaactgca
      481 tcgacccgaa cagttccccg taaacgagcg ccgcgtcgag tggacccatg tcgaacagga
      541 gcgcgagcac aagtgcgcca gccggcagga cgatctcgag acgcacccgg aaccggatga
      601 ggagcgccat gaacaggagc ggcagggaga tgtctatcag tatcatcggc gcctcaagaa
      661 catcgcgaac atcaggtcgg ccgtcccgcc cgggacggtc agcatccccg gtccgtcggc
      721 ggcaacatca tggtgcgctg agatgaactt tgcaatattt tcctcgttct cgacggggag
      781 gtaactgcaa gtgaggtaca gtatcgtgcc gcccggggct agatgacgca gcgccgttcc
      841 gaggcatgtc cgctggcttt cgagaagagt tgcgatctcc ttttcaccca cacgccaccg
      901 gtcctccggg ttgcgccgca ggacgcccgc gccggagcag ggagcgtcga cgagcaccgc
      961 atcgaaccgc gtgtcggcaa gtttttcggg ggcgatggtc gtcaccggcg tcccggcgcg
     1021 cgtcgcccgc tccgttatct cgtcggatcg gtgagtacgg atgtcgcttg ccgttatcct
     1081 gaggtcggac cgctgcgccg cgagcgcgag cgttttgccg ccgccgcccg cgcaaaggtc
     1141 gagcagg
//
LOCUS       DAOQUE010000668         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_183007_length_1145_cov_3.370642, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000668 DAOQUE010000000
VERSION     DAOQUE010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183007_length_1145_cov_3.370642"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PKH10_14005"
     CDS             complement(<1..873)
                     /locus_tag="PKH10_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29279.1"
                     /translation="MKRDILIFLAYLSFFGLLFLQFPLNGALPGNCDSWLLIAISHQY
                     RLFFERLFDPAIGFAMYPVPQVFSYGEATPGCCALFLLLKLFGLTDIWTYYLYLTFIF
                     ALTAWAVYRFAELFMRPFPGALFAGFAFSCCNMTFAHIDDSIIYFYLLPLTAATLLIR
                     HFRERNDALLWGVAALGGMQIYFSLYVFIYQTVLLAAIIVFHRWKRGITIPLRRVVAP
                     VALYLLLPLPYLLFYLHTALSLNYNDPFQLLYTARMTSLMPFDFVAALPENPLYGGLI
                     DLPQNWGFVRHRNFI"
     gene            complement(878..>1145)
                     /locus_tag="PKH10_14010"
     CDS             complement(878..>1145)
                     /locus_tag="PKH10_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29280.1"
                     /translation="LSLRGVVAVIAAIVLWYALFIGVAAPPYGRPFTGVDLVAVTTPI
                     FFLSVAPILLLSRAVPPAGKLFTGYYAALSLILLFAIHLMPLRF"
BASE COUNT          310 a          276 c          408 g          151 t
ORIGIN      
        1 gatgaagttg cggtgccgca cgaaccccca gttctgcggc aggtcgataa gaccgccgta
       61 cagcggattt tcaggcaggg ccgcaacgaa gtcgaacggc atgagcgagg tcatccgcgc
      121 ggtgtagagc agttggaacg gatcgttgta gttgagggaa agagcggtat gcaggtagaa
      181 gagcagatag gggagcggca acaggaggta gagcgcgacc ggagcgacca cccggcggag
      241 cggtatcgtg atgccccgct tccaacggtg aaatacgatg atggccgcca gaagcaccgt
      301 ctgatagatg aagacataca gcgagaaata tatctgcatg ccgccgagcg ccgcaacgcc
      361 ccacaacagg gcgtcgttcc gttcgcggaa gtggcgtatc aggagcgttg ccgccgtcag
      421 cggaagaaga tagaaataga tgatggagtc gtcgatatgg gcgaaggtca tgttgcagca
      481 ggaaaaggcg aatccggcga acagggcgcc ggggaacggc cgcatgaaca gctccgcgaa
      541 ccggtacacc gcccaagcgg tgagcgcgaa gatgaaggtg aggtacaggt aataggtcca
      601 gatatcggta agaccgaaca gtttaaggag cagaaagagg gcgcagcaac cgggggtcgc
      661 ctcgccgtag gagaacacct gcggcaccgg gtacatcgca aagccgatgg cggggtcgaa
      721 caggcgctcg aaaaagagac ggtactggtg ggatatggcg ataagcagcc aggagtcaca
      781 gttgcccgga agtgccccgt tcagcgggaa ctggagaaag agcaggccga aaaaggagag
      841 ataggcgagg aagatgagga tatcccgttt catgcgttca gaacctcaac ggcatgagat
      901 ggatggcgaa caagagtatc aacgagagtg ccgcgtagta gccggtgaac agtttgcccg
      961 ccggtgggac ggcgcgggaa agaaggagga tcggcgccac ggaaagaaag aatatcggcg
     1021 tcgtgacggc gacaaggtcg acccccgtga aggggcgacc gtacggcggg gcggccacac
     1081 cgatgaaaag ggcgtaccag aggacgatag cggcgatgac cgcgaccacg ccgcgcaacg
     1141 agaga
//
LOCUS       DAOQUE010000669         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_184442_length_1140_cov_3.113364, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000669 DAOQUE010000000
VERSION     DAOQUE010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184442_length_1140_cov_3.113364"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PKH10_14015"
     CDS             complement(<1..511)
                     /locus_tag="PKH10_14015"
                     /inference="COORDINATES: protein motif:HMM:NF017321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT29281.1"
                     /translation="MSQPLATSLRPKDLDEVVGQGHITKLISSFLAQGFLPSMIFYGP
                     PGTGKTTVARIAAELFKAKIYYFSGVKDSTVEIKKRVYTEKGTLFAQRQMVFVDELHR
                     FNKAQQDIFLPMIEEGGVIFIGATTENPSFYVNNALLSRTRSMRFEPIGVPDTVAVLK
                     RGLAQLKTTA"
     gene            complement(508..>1140)
                     /locus_tag="PKH10_14020"
     CDS             complement(508..>1140)
                     /locus_tag="PKH10_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29282.1"
                     /translation="LGDKSLGAVRLMEEEGAWAIALVNESVIVQDDRSYSLPVDWRTG
                     RSPDTNLAVLVIDRSAVTARMLAYGLFIAGPDDRSAFDAAFPQVPYVVTARRDTPVTR
                     EAEYPRPVAEYLTKLLKTGAPMTMPEILTAVDRFGRKHKPPADAPKVKVTIPYVERTA
                     ELFVPPVTKDRFVSKEDIEKARVKQRQRAADSDPFKKWAKERPDDDQPRP"
BASE COUNT          181 a          355 c          371 g          233 t
ORIGIN      
        1 cggccgtcgt cttgagttgc gcgagtcccc gcttgagtac cgcgacggtg tcgggcaccc
       61 cgatcggctc gaagcgcatc gagcgggtcc gcgataggag cgcgttgttg acatagaacg
      121 acgggttctc ggtcgtcgcg ccgatgaaga tgacgccccc ctcctctatc atcgggagga
      181 atatgtcctg ctgcgccttg ttgaaccggt gcaactcgtc gacgaacacc atctgtcgct
      241 gggcgaagag cgtccccttc tcggtgtaga cccgcttctt tatctcgacg gtcgaatcct
      301 tgacccccga aaagtaatag atcttcgcct tgaacagttc ggcggcgatc cgggcgaccg
      361 tcgtcttgcc ggtccccggc ggcccgtaaa atatcatcga ggggaggaac ccctgcgcga
      421 ggaacgagga gatgagtttg gtgatatgcc cctgccccac cacttcgtcg aggtctttcg
      481 ggcgcaggct ggtcgcgagc ggttggctca tggtcgcggc tggtcgtcgt cggggcgctc
      541 tttcgcccac ttcttgaacg ggtcgctgtc ggcggcgcgc tggcgctgtt taacgcgcgc
      601 tttttcgatg tcctctttgc tcacgaaacg gtccttggtg accggcggca cgaacagttc
      661 ggcggtccgc tcgacatagg gtatcgtcac tttgaccttc ggggcatcgg cgggcggctt
      721 gtgcttccgt ccgaagcgat cgacggcggt cagtatctcg ggcatcgtca tcggagcgcc
      781 cgtcttgagt agtttcgtca ggtattcggc gaccggtcgg ggatattcgg cctcccgtgt
      841 caccggcgta tcgcggcggg ccgtgaccac ataggggacc tgcgggaatg ccgcgtcgaa
      901 ggcggagcga tcatccggcc ctgcgatgaa caggccgtag gcgagcatcc gggcggtgac
      961 cgccgagcga tcgatgacga ggacggcgag gttcgtgtcc ggcgatcgac cggtgcgcca
     1021 atcgaccggc agtgaatagg aacggtcgtc ctgcacgatg acgctctcgt tgaccagcgc
     1081 tatcgcccac gccccctcct cctccatgag ccgcaccgcg ccgagcgact tatcgccaag
//
LOCUS       DAOQUE010000670         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_186405_length_1133_cov_3.923006, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000670 DAOQUE010000000
VERSION     DAOQUE010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186405_length_1133_cov_3.923006"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PKH10_14025"
     CDS             complement(<1..522)
                     /locus_tag="PKH10_14025"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNT29283.1"
                     /translation="MLIPSIDLMKGKAVQLVQGREKVLERDDPETLAAEFGRFGRIAV
                     IDLDAAFGEGDNEALIRRLCVIAECTVGGGIRTVEKAQKMFSYGAAQVILGSRLFANG
                     AVDVEFAKEVAAAVGRERVIAAIDARDGIVVVKGWKESAGLPVADAVKALDPYVGGYL
                     FTAVEKEGMLQGPA"
     gene            complement(516..>1133)
                     /locus_tag="PKH10_14030"
     CDS             complement(516..>1133)
                     /locus_tag="PKH10_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HNT29284.1"
                     /translation="ITFLDIGATYKSREIMRGIVEAVSRRVFVPLTVGGGIRTVEDMQ
                     AILRSGADKVSLCSAALNDPALLTEGARRFGNQCIVLSIDAKRHGDTWHAYKSGGRTD
                     SGRDAVAWAIEAVERGAGEILLNSIDADGTQAGYDLELLRRVSAAVPVPVIASGGAGK
                     LEHFTAAVREGKADALLLASLLHDGVMTIRQIKEYLRNEEVMVRC"
BASE COUNT          180 a          418 c          337 g          198 t
ORIGIN      
        1 ggcgggaccc tgcagcatcc cctccttctc gaccgcggtg aaaagatagc cgccgacata
       61 gggatcgagc gccttcaccg cgtcggcgac cggcaggccg gccgactctt tccacccttt
      121 caccacgacg atgccgtcgc gggcgtctat cgccgcgatg acccgctcgc ggccgaccgc
      181 cgcggcgacc tctttcgcga actcgacatc gaccgcgccg ttcgcgaaca ggcgtgagcc
      241 gagtatcacc tgcgccgcgc cgtacgagaa cattttctgt gccttctcga ccgtccggat
      301 gccgccgccg accgtacact cggcgatgac gcagagccgc cggatgagcg cctcgttgtc
      361 gccctcgccg aatgcggcgt cgaggtcgat gacggcgatg cggccgaacc ggccgaattc
      421 cgccgcgagc gtttcggggt cgtcgcgctc gagcaccttt tcgcgaccct gcaccagctg
      481 gaccgccttt cccttcatca gatcgatcga tggtatcagc atcgcaccat cacctcctcg
      541 ttccgtaaat attcttttat ctgccgtatc gtcatcacgc cgtcgtgcag gagtgacgcg
      601 aggagcagcg catcggcctt cccctcgcgg accgccgcgg tgaaatgctc cagcttcccg
      661 gcgccgcccg atgcgatgac cggcaccggc accgccgccg atacgcgccg caggagctcc
      721 agatcgtaac ccgcctgcgt cccgtcggcg tctatcgaat tgagcagtat ctcacccgcg
      781 ccgcgctcca ccgcctcgat ggcccatgcc accgcgtcgc gccccgaatc ggtccggccg
      841 ccgcttttgt aggcgtgcca tgtatcgccg tgccgcttgg cgtcgatgga gagaactatg
      901 cactggttcc cgaaccgccg cgccccttcg gtgagaagcg ccggatcgtt cagtgccgcg
      961 ctgcacaacg acaccttgtc ggcccccgag cgcagtatcg cctgcatatc ctcgacggtg
     1021 cggatgccgc cgccgacggt gagcggaacg aagacgcggc gcgagaccgc ctcgacgatg
     1081 ccgcgcatga tctcgcgcga cttgtaggtc gccccgatgt cgaggaaggt gat
//
LOCUS       DAOQUE010000671         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_188350_length_1126_cov_3.035481, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000671 DAOQUE010000000
VERSION     DAOQUE010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188350_length_1126_cov_3.035481"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PKH10_14035"
     CDS             <1..343
                     /locus_tag="PKH10_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNT29285.1"
                     /translation="PWLLAPLTPDPRAVIAALVLWLVIKEARVRPNGRILLAALWYAV
                     TAVLFVFANTLTTAASTIFLQNTAPIWVLLLSVPFLGERPSRAEKLSVPVALGGALLF
                     FSVRRFGGRME"
     gene            354..>1126
                     /locus_tag="PKH10_14040"
     CDS             354..>1126
                     /locus_tag="PKH10_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT29286.1"
                     /translation="MNGNTVEVRGLTKRFGDFYAVNDISFAVRAGEIFGFLGPNGAGK
                     STTIRMLCGILAPTAGEGSVAGFNIMTEQEGIKSAIGYMSQRFSLYDDLTVRENLEFF
                     GSVYGLEGARLRERIAYVMKMADIGRRGDAMVRGLPGGVKQRLALGGAILHDPPVLFL
                     DEPTSGVDPIMRRTFWEIIYDFSRNGKTIFVTTHYMDEAEHCDRMALIIAGKIIALDS
                     PAALKAALPYSVWSLRTERFIEVFEEVAALPFIDEAAIFG"
BASE COUNT          194 a          336 c          370 g          226 t
ORIGIN      
        1 tccgtggctg ctcgcgccgc tgactcccga cccccgggcg gtcatcgccg ccttggtgtt
       61 gtggctggtg atcaaggagg cccgggtgcg ccccaacggc cggatcctcc tggccgccct
      121 ttggtacgcg gtcaccgcgg tgttgttcgt cttcgccaac accctgacca ccgcggcgag
      181 caccatcttc ctgcagaaca cggcgccgat ctgggtgttg ttgttgtcgg tgcccttcct
      241 cggcgaacgg cctagccgcg ccgaaaaact atcggtgcct gtcgccttgg gcggcgcgct
      301 cctcttcttc agcgtgcgtc gcttcggcgg caggatggaa taggagggac gccatgaacg
      361 gaaatacggt ggaagtccgg ggcctcacga agcgtttcgg cgacttttac gcggtaaacg
      421 acatcagttt cgcggtccgc gcgggggaga tattcggttt cctggggcct aacggggccg
      481 gcaagagcac gacaatccgc atgctctgcg gcattctggc accaacggcg ggcgagggga
      541 gtgtggccgg tttcaacatc atgaccgagc aggagggcat aaagagcgcg atcggctata
      601 tgtctcagag gttttcgctc tatgatgacc tgacggtgcg cgagaacctg gaatttttcg
      661 gcagtgtgta cggtctcgaa ggcgcgcgcc tgcgggaacg catcgcgtac gtcatgaaaa
      721 tggcggatat cgggaggcgg ggtgacgcga tggtcagggg gctccccggc ggcgtgaagc
      781 agcgcctggc cctgggcgga gcgattctgc acgatccgcc ggtgctcttt ctcgacgaac
      841 cgacatcggg agtggacccc atcatgcggc gcaccttctg ggagatcatc tacgatttct
      901 cgagaaacgg caagaccatc ttcgtaacca ctcactatat ggacgaggcg gagcactgcg
      961 accgcatggc gctcatcatc gccgggaaga tcatcgcgct tgattctcca gccgcactca
     1021 aggcggccct gccctacagt gtgtggtctc tccgcacgga acgcttcatc gaggtgttcg
     1081 aggaggtcgc ggcgcttccg tttatcgacg aagcggcgat tttcgg
//
LOCUS       DAOQUE010000672         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_189131_length_1123_cov_3.074906, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000672 DAOQUE010000000
VERSION     DAOQUE010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189131_length_1123_cov_3.074906"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKH10_14045"
     CDS             complement(<1..316)
                     /locus_tag="PKH10_14045"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HNT29287.1"
                     /translation="MTVFRTLMLGDVIGRPGRKMLLRTLPSFRERHGIACVVANGENA
                     SGGMGIQRGALDDLFAAGVDVVTSGNHVWHRKEVYGLLAEEPRLLRPANYPARLPGLG
                     RVM"
     gene            complement(330..>1123)
                     /locus_tag="PKH10_14050"
     CDS             complement(330..>1123)
                     /locus_tag="PKH10_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNT29288.1"
                     /translation="FNPVRREITRLTLERLIADGRIHPGRIEELFNAATKEVWKGIKE
                     AGEQALFDLGIHKMHPELVLLIGRLKYRTSYGQNMWKHSVESGFFAGMIAAEMNYNIK
                     MARRAGLLHDIGKAVDHEQEGTHAALGARLAEKYGEAKKIVTAIEAHHNEIKPDSVLD
                     MIVMAADALSGARPGARREMTDSYIQRLTDLENLANAFPGVDKTFAIQAGRELRVMVD
                     AAKIRDEDAYVLANDISKKIEADMTYPGQIKVVVIRETRVIAMAQ"
BASE COUNT          175 a          356 c          363 g          229 t
ORIGIN      
        1 ccatgaccct gccgagcccc ggcaggcgcg ccggatagtt ggccgggcgc aggagccgcg
       61 gttcctccgc gaggaggccg tacacctcct tgcggtgcca cacatggttg ccgctcgtca
      121 cgacatcgac gcccgctgcg aacagatcgt cgagcgctcc gcgctggatg cccatgccgc
      181 ccgacgcgtt ctcgccattc gcgacgacgc aggcgattcc gtgccgctcg cggaaggagg
      241 gaagcgtgcg gagaagcatc ttacgaccgg ggcgtccgat cacatcgccg agcatcaatg
      301 tccgaaaaac ggtcatgcgt ttcgccggat cactgcgcca tcgcgatgac gcgggtctcg
      361 cgaatgacca cgacctttat ctggccgggg taggtcatgt cggcctctat cttcttcgat
      421 atgtcgttgg cgagcacata ggcgtcctcg tcgcgtatct tggcggcgtc gaccatcaca
      481 cgaagttcgc gccccgcctg tatcgcgaag gtcttgtcga cccccgggaa ggcgttcgcg
      541 agattctcga gatcggtgag ccgctggatg taggaatcgg tcatctcgcg ccgcgcgccg
      601 ggccgggccc ctgaaagggc gtcggccgcc atgacgatca tgtcgagcac cgagtcgggc
      661 tttatctcgt tgtggtgcgc ctcgatggcg gtgactatct ttttcgcctc gccgtacttc
      721 tcggcgagcc gggcgccgag cgccgcgtgg gtaccctcct gttcgtggtc gaccgccttg
      781 ccgatgtcgt ggagcaagcc ggcgcggcgc gccatcttga tgttgtagtt catctcggcc
      841 gcgatcatcc cggcgaaaaa gcccgattcg accgaatgtt tccacatgtt ctgaccgtaa
      901 ctggtgcgat acttgagccg accgatgagg agcacgagtt cgggatgcat cttgtggatg
      961 ccgaggtcga acagcgcctg ttcgcccgcc tccttgatgc ctttccagac ctctttggtg
     1021 gcggcgttga acagttcctc gatgcggccg gggtggatgc gaccgtcggc gatgagccgt
     1081 tcgagcgtga gccgcgttat ctcgcggcgc accgggttga agt
//
LOCUS       DAOQUE010000673         1121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_189688_length_1121_cov_3.372420, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000673 DAOQUE010000000
VERSION     DAOQUE010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189688_length_1121_cov_3.372420"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1121
                     /locus_tag="PKH10_14055"
     CDS             <1..>1121
                     /locus_tag="PKH10_14055"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNT29289.1"
                     /translation="DRARPAVAIRDGRLAMCALDRARRILVAHDLEVGAVVTNGILTE
                     DGSMTVKERFSAYWSFRHIWRDDGQEHQIHRAYAKVRLGKFSIEGGRDSVNFGPGEWG
                     LLLSANAEPYWMVKFQNEEPIELFGKWSFMVLNGWLLDTRRDHSDPLLFAARMVYQPL
                     GWLEIGVTRTELYGGEGRPSYKIHEMPEVIFGGKDNVTRGRFDNDGYAALDFTISLPM
                     DRWTDGAVRSLRFYFQEAGTDIAAPWQPEDRIFTIPWIFFHFFERAYLMGLTMSLDDH
                     FFRIEYAKTALSFYRHHLYQEEGYSYRGFSLGYPFGRNSQSIFFKHRWSAAPWLTLEY
                     RLGLYQFPAHSDADHKKEFALAPMFTVKGGATRYYGETT"
BASE COUNT          212 a          343 c          354 g          212 t
ORIGIN      
        1 cgaccgcgct cggccggccg tcgcgatacg cgacggccgc ctcgcgatgt gcgcgctcga
       61 ccgagcgcgc aggatcctcg tcgcacacga cctcgaggtc ggcgcggtcg tgacgaacgg
      121 catcctgacc gaggacggga gcatgacggt gaaggagcgg ttcagcgcct actggagttt
      181 ccgccatatc tggcgcgacg acgggcagga gcaccagata caccgcgcct acgccaaggt
      241 gcggctcggc aagttctcga tagaaggcgg ccgcgacagc gttaacttcg ggccgggcga
      301 atggggcctg ctcctgtcgg cgaacgcgga accttactgg atggtcaagt tccagaacga
      361 agagccgatc gaacttttcg gaaaatggtc attcatggtg ctcaacggct ggctgctcga
      421 cacgcggcgc gaccattcgg acccgctcct ttttgcggcg cggatggtct atcagccgct
      481 cgggtggttg gagatcgggg tcacgcgcac cgaactgtat ggcggcgagg gtcgcccgtc
      541 ctacaagata cacgagatgc ccgaagtgat attcggcggc aaggataatg tgacgcgcgg
      601 ccggttcgac aacgatggct acgcggcgct cgacttcacg atatcgcttc cgatggaccg
      661 ctggaccgac ggcgcggtgc ggtcgctccg gttctatttt caggaggcgg ggaccgacat
      721 cgcggcgccg tggcaaccgg aggaccggat attcaccata ccgtggatat tcttccattt
      781 cttcgagcgg gcctacctca tggggctcac gatgtcgctc gatgaccact tcttccgcat
      841 cgaatacgcc aaaacggcgc tcagtttcta tcgccaccat ctctatcagg aagagggcta
      901 ttcctatcgc ggcttctcgc tcggctatcc gttcggtcgc aactcccaga gtatattctt
      961 taaacatcgc tggtcggccg ctccgtggct gacgctcgaa taccggctcg gcctgtacca
     1021 gttcccggcc cacagcgacg cggatcataa aaaagagttc gcgctggcgc cgatgttcac
     1081 ggtgaaaggc ggtgcgacgc ggtactatgg cgagacgacg c
//
LOCUS       DAOQUE010000674         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_191411_length_1115_cov_5.063208, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000674 DAOQUE010000000
VERSION     DAOQUE010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191411_length_1115_cov_5.063208"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKH10_14060"
     CDS             complement(<1..322)
                     /locus_tag="PKH10_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29290.1"
                     /translation="MRVPIVTVIALFFLLLVVACDKSATQGPCDGIDCSGHGTCDATS
                     GRAVCICDENYHPNGLLECIADTDPCEGVDCDEWESCVNGSCDPLPGKCNDKDDCGPN
                     EACED"
     gene            complement(399..>1115)
                     /locus_tag="PKH10_14065"
     CDS             complement(399..>1115)
                     /locus_tag="PKH10_14065"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNT29291.1"
                     /translation="TALKTYRYETSNLNSAPADFTTPHGGLPQTSATCPAGTYANDSV
                     MLTLQLKVPTNALSFSFNFRFFSQEYWKWTCDTDGYNDFFIAMLYTGAAGIPADKNIS
                     FDQNSSYISVNSSAFFTVCEAKSGYTCPDGASALAGTGYGEKYYYDATNYDMGGGTKW
                     LTTAAPVVPGETITLKFVIWDTNDRDRDSLVIIDNFKWSAQGTSGPSTFECWDLNQNG
                     NCDIATEDKSGDETCNERDC"
BASE COUNT          202 a          308 c          365 g          240 t
ORIGIN      
        1 ggtcctcgca cgcctcgttc ggcccacagt cgtccttgtc gttgcatttc cccgggagcg
       61 ggtcgcagga gccgttcacg cagctctccc actcgtcgca atcgaccccc tcgcacggat
      121 cggtgtcggc gatgcactca aggagaccgt tcgggtgata gttctcgtcg cagatgcaga
      181 cggcgcgtcc gctcgtcgca tcgcaggtgc cgtggccgga gcagtctatc ccgtcgcagg
      241 gaccttgcgt ggcgctcttg tcgcaggcga cgacgagcag aaggaaaaaa agcgcgataa
      301 cggtgacgat agggacgcgc atgaggggcc tccgttgcgg tgacggccta ttgtcgccgg
      361 aacgggtgtc ggtgtcaaga ggaagatgtc agaagaggtc agcagtcgcg ctcgttgcag
      421 gtctcgtcgc ccgacttgtc ctcggtcgcg atatcgcaat tgccgttctg gttgaggtcc
      481 cagcactcga aggtggaggg accgctcgtt ccctgcgcgc tccatttgaa gttgtcgatg
      541 atgaccaacg aatcgcgatc gcggtcgttg gtgtcccata tcacgaactt gagcgttatc
      601 gtctctcccg gcacgaccgg cgcggcggtc gtgagccact tggtgcctcc gcccatgtcg
      661 tagttcgtag catcataata atacttttct ccatagcctg ttcctgcaag agcggaggca
      721 ccatcgggac aggtatagcc gctcttcgcc tcgcagacgg tgaagaaggc actcgagttg
      781 acgctgatgt aactgctgtt ctggtcgaac gatatgttct tgtcggccgg aatgcccgcc
      841 gcgccggtgt aaagcatcgc gatgaagaag tcgttgtagc catcggtgtc gcaggtccat
      901 ttccagtatt cctgcgagaa gaagcggaag ttgaacgaga aggagagggc gttggtcggc
      961 accttgagtt gcagggtgag catcaccgaa tcgttcgcgt aggtcccggc ggggcaggtg
     1021 gcgctcgtct gtggcaggcc gccgtgcggc gtggtgaaat cggcaggggc cgagttaagg
     1081 tttgaggtct cgtaacgata ggtcttcaat gcggt
//
LOCUS       DAOQUE010000675         1110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_192947_length_1110_cov_3.624645, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000675 DAOQUE010000000
VERSION     DAOQUE010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192947_length_1110_cov_3.624645"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKH10_14070"
     CDS             complement(<1..586)
                     /locus_tag="PKH10_14070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNT29292.1"
                     /translation="MCFSLRAGIIQALFSFRRCSAVSEERLLQDRFLLVRQLGTGGMG
                     EVWLAEDAERHETIAVKMIRNKGLLRRAEVKRLRAEFQIARSIKSEYVVRMFDLYDEK
                     KEVFFTMEFVEGRTFATLVGSKLADVIRSFLLLTRGLLDLHNYNIVHRDLKPSNIMIT
                     PQDAVKILDFGLASLKMMHAKERSGTARYMAPEVF"
     gene            848..>1110
                     /locus_tag="PKH10_14075"
     CDS             848..>1110
                     /locus_tag="PKH10_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29293.1"
                     /translation="MKKNDLTMLLLSFLLVAVVACKQETTKNDADTVMTDSDIIAVED
                     DMVAIDDGGVVEDEIVSDDTILISDTDADTHIGPGDKEPVEVD"
BASE COUNT          257 a          288 c          327 g          238 t
ORIGIN      
        1 ggaagacctc gggcgccatg tagcgggcgg tgccgctccg ttccttggcg tgcatcatct
       61 tgagcgaggc gagcccgaag tcgagtatct tcaccgcgtc ctgcggggtt atcatgatgt
      121 tcgacggctt gaggtcgcgg tggacgatgt tgtagttgtg caggtccagc aggccgcgcg
      181 tcaggagcag gaaggaccgt atcacatcgg cgagtttcga gccgacgagc gtcgcgaagg
      241 tgcgcccctc cacgaactcc atcgtgaaga agacctcttt cttctcgtcg tacaggtcga
      301 acatccgcac cacatactcg ctctttatcg agcgggctat ctggaactcg gcgcgcaggc
      361 gcttcacctc ggcgcggcgc agaagtccct tgttgcggat catcttgacc gcgatcgtct
      421 cgtgccgttc ggcgtcctcg gcgagccaca cctcgcccat gccgccggtc cccagttggc
      481 gcacgaggag aaaacgatcc tgcaggagcc gctcttcaga caccgccgaa caccgcctaa
      541 aagaaaacaa cgcctgaatg ataccggcgc ggagcgaaaa acacaagaaa aaggaagccg
      601 aatgttttcg gcgacatcgt ggtaacaaag gagaatcact cttgcttctt tatcgttgcg
      661 gtccttcctg cccggagaag gaatacctga acaaataagg ggagatgata agttttctga
      721 aaaaaggaga atccttttga gcaccgcagg tatcttatag atagaacgga aaaaaggtgg
      781 ttgctttttg ttttcggagt gatatgatcg ccgcatatct tatcctgatc agggggcgca
      841 gagacctatg aaaaagaacg acctgacgat gttgttgctt tcgttcctgc ttgtggcggt
      901 ggtcgcctgc aagcaggaaa cgacgaagaa tgatgccgac acggtgatga ctgacagtga
      961 catcatcgct gtggaggatg acatggtcgc tattgatgac ggtggtgttg tcgaagatga
     1021 gatcgtatcg gacgacacca ttctgatttc tgataccgat gccgacaccc acatcggtcc
     1081 cggtgacaag gagccggtcg aggtggatga
//
LOCUS       DAOQUE010000676         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_193259_length_1109_cov_2.767552, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000676 DAOQUE010000000
VERSION     DAOQUE010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193259_length_1109_cov_2.767552"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1109
                     /locus_tag="PKH10_14080"
     CDS             <1..>1109
                     /locus_tag="PKH10_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT29294.1"
                     /translation="GKGIELNDGVQAILLETLVDECTYGGISVSGVDGGAGAALSADL
                     LVVRGTGPSPNTGKWGYGIAAIDHAALKLTRVLVSDNRAFGVAAMGVEEKRLGTAELS
                     EVLILDTRAADDDGLYPPRGLDIDNGVTASLERVAVLRSANVGVMLMGPLSPTVPLTV
                     TARDLTVRDTVEEPFGRIDGQGIVLLNETDVTLERALIENNRAFGLLVSVYEEGDYTT
                     HLTATDLTVRGTRPQALDDGAGIGVGVYRHAEVTLERALIEDNRTAGLLLYGAESGSC
                     DATLTAKDLTVRGTTGQAADGEHGVGVAVQYGARAMFERALMEENRMYGVMVFGEGAG
                     PEPMLDLKESTVRRTLPRDCLDSPAGCSFAPGVPIA"
BASE COUNT          186 a          329 c          407 g          187 t
ORIGIN      
        1 ggcaaaggga tcgagttgaa cgacggggta caggcgatat tgttggaaac actggtcgac
       61 gagtgcacct acggcggtat cagcgtgtcg ggggttgacg gcggcgcggg agcggcgttg
      121 tcggccgacc tgctcgtggt gcgcggcacc ggaccgtcgc ccaacaccgg caagtggggc
      181 tacggtatcg ccgcgataga ccatgccgcg ctgaaactca cccgggtcct tgtttcggat
      241 aaccgcgcct tcggtgtcgc ggcgatggga gtggaggaaa agcggctcgg taccgcggag
      301 ctctccgagg tcctgatact tgatacgcgg gcggctgatg acgacgggct ctacccgccg
      361 cgggggctcg atatcgataa cggggtcacg gcgtcgctcg aacgggtggc ggtcctccgt
      421 tcggcaaatg tcggtgtcat gctgatgggg ccgctgtcac cgacggtgcc cctgacggta
      481 acggcgcgcg atcttaccgt tcgcgatacg gtcgaggagc cgttcggccg catcgacgga
      541 caggggatcg tcctgctcaa tgagaccgat gtcacgctgg agcgggcgct catcgagaat
      601 aaccgcgcct tcggcctgct cgtatcggtg tacgaagagg gcgactatac cacgcatctg
      661 acggccaccg accttacggt gcgcggcact cgcccacagg cgctcgacga cggcgccggt
      721 atcggcgtcg gcgtctatcg tcatgccgaa gtgacgctcg aacgggcgct catcgaggat
      781 aaccggaccg ccggcttgct tctgtacggc gccgagtcgg gttcctgcga tgcgacactg
      841 acggcgaaag acctgacggt gcgggggacg accggtcagg cggccgacgg ggagcacggc
      901 gtcggtgtcg cggtgcagta cggcgcgcgg gcgatgttcg aacgggcgct gatggaagag
      961 aaccggatgt acggggtcat ggtctttggt gaaggcgccg gcccggagcc gatgctcgac
     1021 ctgaaggaaa gcacggtccg gcggaccctg ccgcgtgact gcctcgatag tccggcgggg
     1081 tgctccttcg cccccggcgt gccgatagc
//
LOCUS       DAOQUE010000677         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_193480_length_1108_cov_4.075024, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000677 DAOQUE010000000
VERSION     DAOQUE010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193480_length_1108_cov_4.075024"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PKH10_14085"
     CDS             complement(<1..844)
                     /locus_tag="PKH10_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase PrkA"
                     /protein_id="HNT29295.1"
                     /translation="MAKKSVNAPVPGDAGLLGHVQAVIAGERRFENVFQAVSRMILGD
                     PSKIEKVTVNGRSTYDFKVFREGKKHIIGMFDEINSFVSFVKDAAEGGSSSEMAFVLI
                     GEPGNGKTFFVDFLSGLYRQFLMKPENRRWTFRFTGLDKLGGYGKIERIESQTFEDPM
                     VLAMNLFESAEENKAFLAKSGATDKQIAAFWKNYRPLGACSYYIWEQIRAHCGGDLAR
                     MLEFVEVIPVPVSESRGVITGKYAAKDKITSSAVDLLGEESITRLLHISATDNPYRFD
                     LRRGA"
BASE COUNT          211 a          334 c          332 g          231 t
ORIGIN      
        1 gcgcgccgcg ccgcaggtcg aaccggtacg ggttgtcggt cgcgctgatg tgaaggagcc
       61 gcgtgatgga ttcttcgccg agcagatcga ccgccgaaga ggttatcttg tctttcgccg
      121 cgtatttgcc ggtgatgacc ccgcgcgatt cgctgaccgg caccgggatc acctcgacga
      181 actcgagcat cctggcgagg tcgccgccgc agtgggcgcg gatctgctcc cagatgtagt
      241 aagagcaggc gccgagcggc cggtagttct tccagaacgc ggctatctgt ttgtcggtcg
      301 cgccgctttt cgcgaggaac gccttattct cttcggccga ctcgaacagg ttcatcgcga
      361 gcaccatcgg atcctcgaag gtctgcgatt cgatgcgctc tatcttgccg tagccgccca
      421 gtttatcgag cccggtgaag cggaaggtcc agcgccggtt ctccggtttc atgaggaact
      481 ggcggtagag gcccgacagg aaatcgacga agaaggtctt gccgttgccc ggttcgccga
      541 tgaggacgaa ggccatctcg gacgacgagc cgccctcggc cgcgtccttg acgaacgaaa
      601 cgaacgagtt gatctcgtcg aacatcccga tgatgtgctt cttcccttcg cggaacacct
      661 tgaagtcgta ggtggaccgg ccgttcaccg tcaccttctc gatcttcgac gggtcgccga
      721 ggatcatccg cgagaccgcc tggaagacat tctcgaaccg ccgctcgccg gcgatgaccg
      781 cctgcacatg accgagaaga ccggcatcgc cggggaccgg ggcattgacg cttttcttcg
      841 ccatctcgct gtctcccttt ttcaatccgc caccgttacc ggcccatcgt aaagggttag
      901 cgggatgaag tcaagaagaa cgggggaaga agcagttgct tttcgttccc acgacgctat
      961 aatcgttttc tatcgtatcc ggtgggcgca gagacctatg aaaaagaacg acctgacgat
     1021 gttgttgctt tcgttcctgc tcatggcggt ggtcgcctgc aatctacaca ccgcaaagaa
     1081 tgacgccgat acggtgaccg cggacgac
//
LOCUS       DAOQUE010000678         1103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_195041_length_1103_cov_3.711832, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000678 DAOQUE010000000
VERSION     DAOQUE010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195041_length_1103_cov_3.711832"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..930
                     /locus_tag="PKH10_14090"
     CDS             304..930
                     /locus_tag="PKH10_14090"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT29296.1"
                     /translation="MITAQVSIFAFYAARVILYLLFVVSFFTLAFFVERLLYYRRTLV
                     RDADRLQTELFAAPDRARMLAVLHAEKGEEARMIVESFGEGIVGPEAFRELVTARLIA
                     ARQRWERYTDFLGTVGSNAPFVGLLGTVLGIMKSFADLAVATKGGPQAVMSGISEALI
                     ATAVGLGVAIPAIIFFNICKGRIRRSSALVEALQARVSAGISETGEGK"
BASE COUNT          235 a          312 c          318 g          238 t
ORIGIN      
        1 ggggcgcctc tgcgggtatc gccggcggct cggcgggggt ctctatcgtt atctcgtcta
       61 tcaccgtgtg cccggtgtcg ggcgcatcct gtgccgacag catagaaccg gcaacaagaa
      121 aaataaaaaa ggtgacgaaa ggtctgctcc tcaatcgggc ctctttcaag agaacgggcg
      181 ctctctacca caaaggggcg accgaatcaa tacctttgtt ctcgtttctt gcccggagaa
      241 agataaaaaa ctggaaaatg tggctccccg ctggtatagc gatacgaacc tacggaggtc
      301 cccatgatca cggctcaggt ctcgatcttt gccttctacg ccgctcgcgt catactgtat
      361 ctgctcttcg tcgtctcctt tttcacgctc gccttcttcg tggagcgtct gttgtactac
      421 cggcggaccc tggtgcgtga tgccgaccgt ctgcagacgg aactcttcgc cgctcccgat
      481 cgggcgcgga tgctggcggt gctccatgct gaaaaggggg aagaggcccg catgatagtc
      541 gagtcgttcg gcgaggggat cgtcggcccc gaggcgttcc gggaactggt caccgcccgg
      601 ctcatcgcgg cccgtcagcg ttgggagcgt tacaccgatt tcctcgggac ggtcggatcg
      661 aacgcgccgt tcgtcgggct tctcggcacc gtgctcggca tcatgaagag tttcgccgat
      721 ctcgcggtcg ccaccaaagg cggcccgcag gcggtcatga gcggcatttc cgaggcgctt
      781 atcgccacgg cggtcggtct gggcgttgcc atcccggcca ttatcttctt caatatctgc
      841 aaaggccgca tccggcgcag ttccgccctc gtcgaggcgc ttcaggcgag ggtcagcgcc
      901 ggtatcagcg aaaccgggga aggaaagtaa aaaatatccc atagaaatca agcagttgtt
      961 aattgtataa aaaaaatccg agaaaagtgt tgacaaagaa atcgctcctg ctatagtcat
     1021 cgagcgtttt tattagcgca ctggtttcga gttcattgaa aactgagcag cagcaaagag
     1081 agagaaaata aggaaccaac cct
//
LOCUS       DAOQUE010000679         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_195357_length_1102_cov_3.376313, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000679 DAOQUE010000000
VERSION     DAOQUE010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195357_length_1102_cov_3.376313"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKH10_14095"
     CDS             complement(<1..353)
                     /locus_tag="PKH10_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29297.1"
                     /translation="MAPFRRRHLLGATLLLIALVACEPKEDTLAVGEGFDAFSEGADL
                     VLWGADHERRDIDGKGFECAGGGMTCRRIFVARFKKNFERTAFFFQEHPVTDIAATLT
                     ARTAGGYLLATTRKR"
     gene            complement(355..>1102)
                     /locus_tag="PKH10_14100"
     CDS             complement(355..>1102)
                     /locus_tag="PKH10_14100"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HNT29298.1"
                     /translation="GTEVGRWKLFDILDPYRLGYTSIRMFKQWSHANGLLYDESDDSF
                     IVSSRNQSVLLKLGRADGQLKWLLGPHDNWKEPWSDLLLAPVGEPFVWNYYQHGPMLR
                     ADGSVLVFDNGAYRAFPYEPMFPAEDNWSRAVIFSVDETARTVTQVWEWGSDIGEKIY
                     APYLGNAAELPATGNVLVVFGGITHGPDGQPTDDLLDCKVSARVIEVTPGEGASEKVF
                     DMGIKDTDEGLQGWRIYRGDHWPSLYAGAQ"
BASE COUNT          211 a          366 c          320 g          205 t
ORIGIN      
        1 cagcgcttcc gcgtcgtcgc aagcagatac ccccctgccg tgcgggcggt cagggtcgcg
       61 gcgatatcgg tcacgggatg ttcctgaaag aaaaaggcgg tccgttcgaa attcttcttg
      121 aatcgggcca cgaagatacg acggcaggtc attccccctc cggcgcattc gaaccccttg
      181 ccgtcgatat cccttcgctc gtgatcagca ccccaaagta cgagatcggc cccctcggag
      241 aaggcgtcga acccctcgcc gaccgccagc gtatcttctt tcggctcaca ggcgacgagc
      301 gcgatgagga gcagggtcgc cccgagaaga tgtcggcggc ggaacggcgc catctcactg
      361 tgctccggcg tagagcgacg gccaatgatc accgcgatag atacgccagc cctgcaggcc
      421 ctcatccgta tccttgatcc ccatgtcgaa caccttttcc gatgccccct ctccaggggt
      481 gacctcgatg acgcgcgccg acaccttgca atcgagcagg tcgtcggtcg gttgaccgtc
      541 gggtccgtgg gtgataccgc cgaacacgac gagcacattg ccggtcgccg ggagttccgc
      601 ggcattcccg aggtagggtg catatatctt ctcgccgatg tcgctccccc actcccagac
      661 ctgcgtcacg gtgcgcgccg tctcatcgac cgagaatatc acggcgcggc tccagttatc
      721 ctccgccggg aacatcggtt cgtaggggaa ggcacggtag gcgccgttgt cgaacaccag
      781 taccgacccg tcggcgcgaa gcatcggccc gtgctggtaa tagttccaca cgaacggttc
      841 accgaccggc gcgagcagaa gatcgctcca cggctccttc cagttatcgt gcggcccgag
      901 cagccatttc aactgaccgt cggcacggcc cagtttcagg agcaccgact ggttgcggct
      961 agacacgatg aaggaatcgt cgctctcgtc gtacagcaga ccgttggcat gactccactg
     1021 cttgaacatc cggatggagg tgtatccgag acggtacgga tcgaggatat cgaagagttt
     1081 ccatctcccg acctcggtcc cc
//
LOCUS       DAOQUE010000680         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_195386_length_1102_cov_2.828080, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000680 DAOQUE010000000
VERSION     DAOQUE010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195386_length_1102_cov_2.828080"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..>1102)
                     /locus_tag="PKH10_14105"
     CDS             complement(107..>1102)
                     /locus_tag="PKH10_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT29299.1"
                     /translation="RKRTVEQIRKLLSLGILGPEPDDTPPARWEVPVWARPMTKEGRL
                     ADTVPVPPPSPDKKVAPSPALPGETADLLPVRPVAPPPTPTPAPAPSAPASPVPRPVE
                     PPAEKNAPPEDDKDNDSFIESLYQDEPAPSRSSGGRTLLIAALLIAIAGGGTALWYVF
                     SQSVEEEPPAEDTVAVVPDTVVIAPVEPPPPPDPFAGKSADELLKIGIEGHKTEQYAA
                     AIAAYRAALKALPADAEPVRAAAIWKNLAISAYEGDDQPTALDAIEQALTVDIDADKI
                     ALKAAILEEMGRFDDAMNAYRAFLKDKRFKAKKKEWIKEITRLKKDKKQAEKEKK"
BASE COUNT          149 a          322 c          386 g          245 t
ORIGIN      
        1 ttgagcacca cgagcagggt gaacggggta tctccgtagg cggcgcggat gtctgccgcc
       61 atctcggtga cgcggcgttg aatggtcgtt tcgtcgagga gcaccttcac ttcttttcct
      121 tttccgcctg ttttttgtct tttttaaggc gggttatctc cttgatccac tcctttttct
      181 tcgccttgaa ccgcttgtcc ttgaggaagg cgcggtaggc gttcatcgcg tcatcgaacc
      241 gccccatttc ctcgagtatc gccgccttca gcgctatctt gtcggcgtcg atgtccacgg
      301 tgagcgcctg ctcgatcgca tcgagcgcgg tcggctggtc gtcgccttcg taggccgaga
      361 tggcgaggtt cttccatatg gcggcggccc gtaccggctc cgcgtcggcg ggcagggctt
      421 taagcgccgc gcggtaggcg gcgattgcgg cggcgtactg ttccgttttg tgaccctcga
      481 tgccgatctt gagcaattcg tcggcgcttt ttccggcgaa cggatcgggg ggaggcggcg
      541 gttcgaccgg ggcgatgacg acggtgtcgg ggaccacggc gacggtatct tcggccggtg
      601 gttcctcttc gaccgactgg gagaatacat accagagcgc cgtgccgccg ccggcgatcg
      661 cgataaggag cgcggcgatg agcagggtcc tgccgcccga tgatcgcgac ggggccggct
      721 cgtcctgata aagactttct atgaaggaat cgttatcttt gtcgtcttcg ggcggcgcgt
      781 tcttttcagc cggtggctcg accggtcgcg gcaccggtga ggcaggtgcc gaaggggcag
      841 gggccggagt cggtgtcggc ggtggcgcga ccggcctgac cggcaggagg tcggcggtct
      901 cgcccggtag cgccggggaa ggggcgacct ttttatcggg cgacggcggc gggaccggta
      961 cggtgtcggc aagacgccct tctttggtca tgggccgcgc ccataccgga acttcccatc
     1021 gggcgggggg cgtatcgtcc ggttcggggc ccagaatgcc cagagaaagc agtttccgta
     1081 tctgctcgac ggtccgtttc cg
//
LOCUS       DAOQUE010000681         1092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_198505_length_1092_cov_2.481196, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000681 DAOQUE010000000
VERSION     DAOQUE010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198505_length_1092_cov_2.481196"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PKH10_14110"
     CDS             complement(<1..711)
                     /locus_tag="PKH10_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29300.1"
                     /translation="MRSRLFLIAALAAVFPFFIIHAEVSFEGDIAFGGSYNSNMALLS
                     SGDRVTMDARKAGLDVFSLDLDAFLTLIPADIFSLEYTLFAVVPPQPAHISYAFFNHA
                     LAVLFTHEFDEVDISYGVELGHLLIGFENRLLEPQVLFDLFWYARESLSYYLSGSFSY
                     AASLDREYAYLTAPGFRFENGVYIYPVSGDRSFISVGVGERMFFFGEDHILSPEPPAS
                     PEPEVWARRDLSETYLRLK"
     gene            complement(730..1020)
                     /locus_tag="PKH10_14115"
     CDS             complement(730..1020)
                     /locus_tag="PKH10_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29301.1"
                     /translation="MRNALLAILFLSLLPILPLAAQEDQPVKPSSEIEPGKDAPGWEA
                     RFRKEKGKEHAEEKKERRKEMKKEKKKEHKEEDRDQKKEQRHERKREHSEHR"
BASE COUNT          253 a          297 c          303 g          239 t
ORIGIN      
        1 cttcaggcgc agataggtct ccgagagatc gcgacgagcc cacacttcgg gttcgggtga
       61 cgcgggcggc tccggcgaaa ggatatgatc ctcgccgaaa aagaacatcc gttcccccac
      121 tccgaccgag ataaaactac ggtcaccgct gaccgggtag atgtagacgc cgttctcgaa
      181 acggaagccg ggcgccgtaa gatacgcata ctcgcgatcc agacttgccg cataggaaaa
      241 ggaaccgctc agataatagg atagcgattc tcgggcgtac cagaaaaggt cgaacaacac
      301 ctgcggttca aggagccggt tctcgaagcc gatcagcaga tgacccaatt cgaccccgta
      361 actgatatcg acctcgtcga attcatgcgt aaaaagaacg gcgagcgcat gattgaagaa
      421 ggcgtaggat atgtgcgccg gttgaggcgg gaccaccgcg aaaagggtgt attccaaaga
      481 aaagatgtcg gcgggtatca gtgtcaggaa cgcatcgagg tcgagcgaga agacatcgag
      541 tcccgctttt cgcgcatcca tcgtcacgcg gtccccggac gagaggagag ccatattact
      601 attataagaa ccaccgaaag caatatcgcc ctcgaaggaa acctcggcgt gaatgatgaa
      661 aaaaggaaat accgcggcaa gcgcggcgat gaggaacagg cgggatctca tcaggcgtgt
      721 tgcttcttcc tagcgatgtt cggaatgttc tctttttcgt tcgtgtcgct gttctttttt
      781 ctggtcccgg tcttcttcct tatgttcttt cttcttttcc tttttcatct ccttgcggcg
      841 ttctttcttt tcctcggcat gctccttgcc cttctctttc ctgaaacgcg cctcccatcc
      901 gggggcgtcc tttcccggct cgatctcgga ggagggcttg accggttggt cctcctgcgc
      961 cgcaagcggg aggataggca ggagagaaag gaacagtatc gcgagcaagg cgttacgcat
     1021 gggtctcctc cttcaccaac agggtaacga cattttcgac aacgcttccg ttgaaacggg
     1081 catgggcccg ta
//
LOCUS       DAOQUE010000682         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_200647_length_1085_cov_2.974757, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000682 DAOQUE010000000
VERSION     DAOQUE010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200647_length_1085_cov_2.974757"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1085)
                     /locus_tag="PKH10_14120"
     CDS             complement(<1..>1085)
                     /locus_tag="PKH10_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29302.1"
                     /translation="DAVECPGIPCRDTDKDGVPDYRDKDSDEDGIPDAVEAGTAPTTP
                     IDSDLDGDADYRDIDADNDGIKDLYEGRKDPDGDTIPNYLDLDSDGDGVPDCIEGRGT
                     TFKDTDMDGQPDCKGKPDYADPPADTDTDDRYDFVDTDSDGDGLGDDQEVICPNLQKD
                     GRVWADVDGDGFSDLAERAVGSKVCDPDEGVTDMPGVDFYFELPYEEPEKTDTLLFTP
                     SVQKADIFFNIDTTGSMDGELYNLKHMLSSTIIPQTRQRISDSAFGVSQFKDEDELSL
                     IQIDPVTDTMAAQLALSALTEGYGGDCPEAGYFSLHNLGANGQWRPGAIPLAVHITDA
                     PSHERGADKASAFAALGAMGTKVITVL"
BASE COUNT          159 a          347 c          344 g          235 t
ORIGIN      
        1 aaggacggtg atgaccttgg tccccatggc gccgagcgcc gcgaacgccg acgccttatc
       61 ggcgccccgt tcgtggctcg gcgcgtcggt gatgtggacc gcgagcggta tcgcacccgg
      121 ccgccactgg ccattcgccc cgaggttgtg caaggagaaa taacccgcct cgggacagtc
      181 gccgccgtac ccttccgtca gggcgctgag ggccaactgg gccgccatgg tgtcggtcac
      241 cgggtcgatc tgtatgagcg aaagttcgtc ctcgtccttg aactgcgaga ccccgaaggc
      301 gctgtcgctt atcctctgcc gcgtctgcgg gatgatggtg ctcgacagca tgtgtttcag
      361 gttgtagagt tcgccgtcca tcgaaccggt ggtgtcgatg ttgaagaata tatccgcctt
      421 ctgcaccgac ggggtgaaca ggagcgtatc ggtcttctcc ggttcctcat agggcagttc
      481 gaagtagaaa tcgaccccgg gcatgtcggt gaccccttcg tcggggtcgc agaccttcga
      541 tccgaccgcc cgttcggcca ggtcgctgaa cccgtcgcca tcgacatcgg cccatacgcg
      601 accgtccttc tgcaggttcg ggcagatcac ctcctgatcg tcgccgagcc cgtcgccgtc
      661 actatcggta tccacgaagt cgtaacggtc gtcggtgtcg gtgtcggccg gcggatcggc
      721 ataatcgggc tttcccttgc agtcgggctg accgtccatg tcggtgtcct tgaatgtcgt
      781 gcctcggccc tcgatgcagt cgggcacccc gtcgccgtcg ctgtcgaggt cgagatagtt
      841 gggtatcgtg tcgccatcgg gatccttgcg cccttcgtac aggtccttga tgccgtcgtt
      901 gtcggcatcg atgtcacgat aatcggcgtc accgtccaga tcgctgtcga tcggggtggt
      961 cggggcggtc ccggcctcca cggcgtcggg gatgccgtcc tcgtcgctgt ccttgtcgcg
     1021 ataatcgggt acgccgtcct tatcggtatc ccggcacggg atcccgggac actctaccgc
     1081 atcgg
//
LOCUS       DAOQUE010000683         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_204333_length_1073_cov_4.269155, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000683 DAOQUE010000000
VERSION     DAOQUE010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204333_length_1073_cov_4.269155"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1073
                     /locus_tag="PKH10_14125"
     CDS             <1..>1073
                     /locus_tag="PKH10_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29303.1"
                     /translation="LSFFAIFFLIVCGGSNVTIVADTDALLLGDEDPLLGDEDGIVPT
                     DEEEPEGEGIVTKDDAPIAKDDAPVVTDDAPVVTDDAPVVTDDDTGQPDEEQPDEEQP
                     DEELTDETIPDEFFIDEEVTDDVVTDDVVTDELLTDDDTITTYTIGWCNLQWPTDPTV
                     TSGDDITVYGRVYVAGLTDLSTAVDSAEELVVQMGLGTIGTDAETWNWGSQNIEPNPD
                     NASAPEIGENDEYIFSTTADLPAGTYAFTFRFSGDGGATWTYCNANRPGDGYNGTDAE
                     HPFEENKAGLLTVESGYVDPCLDPDACTEEHRGVCTDADLDGVAECACDLTYHLDTNT
                     GTCVSDTRNMPCTNLLPLNASWK"
BASE COUNT          236 a          321 c          316 g          200 t
ORIGIN      
        1 cctctcgttt ttcgctatct ttttcctgat cgtctgtggc ggcagcaacg tcacgatcgt
       61 tgctgacacc gatgcgctgc tgctcggcga tgaggatccc cttctcggtg atgaggatgg
      121 gatagtcccg accgacgagg aagagcccga gggtgagggt atcgtcacca aggatgacgc
      181 ccctatcgcc aaggatgatg ccccggtggt gaccgacgat gccccggtag tgaccgacga
      241 cgccccggta gtgaccgacg acgatactgg tcagccggat gaggaacagc ccgacgaaga
      301 gcaacccgat gaggaactga cggacgaaac gatccccgac gagttcttca tcgacgaaga
      361 ggtgaccgat gatgtggtca ccgatgatgt cgttaccgac gaacttctca cggacgacga
      421 cacgataaca acctacacga tcggctggtg caatctgcag tggcccaccg acccgaccgt
      481 tacttccggt gatgacatca ccgtgtacgg tcgcgtctat gtcgcggggc ttaccgatct
      541 ttcaacggcg gtcgattcgg cggaggaact ggtagttcag atggggctcg gcactatcgg
      601 caccgatgcg gagacatgga actggggaag ccagaacata gagccgaatc ccgacaacgc
      661 cagcgcccca gagatcggcg agaacgatga atacatcttc tcgacaacgg ctgatcttcc
      721 ggccgggaca tacgctttca ccttccgttt ctcgggcgac ggcggcgcca cctggaccta
      781 ctgtaacgcg aaccgcccgg gcgatgggta caacgggacc gatgccgaac accccttcga
      841 agagaacaag gcgggactcc ttacggtcga gtcgggctat gtcgatccct gcctcgatcc
      901 ggatgcctgc accgaagagc acaggggcgt atgcaccgat gccgaccttg acggcgttgc
      961 cgaatgcgcc tgcgatctga cctatcatct ggataccaat accggtacct gtgtctccga
     1021 cacgcggaac atgccctgca ccaatctact tccgttgaat gcatcgtgga agg
//
LOCUS       DAOQUE010000684         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_207577_length_1063_cov_3.067460, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000684 DAOQUE010000000
VERSION     DAOQUE010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207577_length_1063_cov_3.067460"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..549)
                     /locus_tag="PKH10_14130"
     CDS             complement(49..549)
                     /locus_tag="PKH10_14130"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNT29304.1"
                     /translation="MNLSSRFLKKDTAARSEQPLQFISFFVGDEIYATDILKIHEITN
                     YRDLTRIPSAPSFVKGVLDLKGIAVPVMDLREKFALPARPYSKFSVIMVVDVSGRIMG
                     VIVDSVADVLQIDRGDIQPAPKFSATLKAEFIKGMIKQGDNRFIVLLDMDRMLSDREL
                     DMVESL"
     gene            complement(546..>1063)
                     /locus_tag="PKH10_14135"
     CDS             complement(546..>1063)
                     /locus_tag="PKH10_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNT29305.1"
                     /translation="LRGAIEVYSEKGSGTLFRLKLPLTLAIIDGMRVAVGSEILTIPL
                     LSIIEAIRPAPDGIKTIEGKGELIEFRGEYLPLIRLYEILGFDDAITNPEEAIVIILE
                     SGIRRMALMVDDVIGQHQAVIKSLETNFRRVEGVSGATILGDGRVSIILDIHGIEHLA
                     FGGHRTSEVVA"
BASE COUNT          196 a          282 c          358 g          227 t
ORIGIN      
        1 cggggagcag gtcctgaaag gcgttctggt cgttaccgtg cccggcggtc atagcgattc
       61 caccatatcg agttcgcggt ccgaaagcat gcggtccatg tcgagtagga cgatgaagcg
      121 attgtcgccc tgttttatca tccccttgat gaactcggcc ttgagcgtgg ccgagaattt
      181 aggcgccggc tggatgtcgc cgcgatcgat ctggaggacg tcggcgacgc tgtcgacgat
      241 gacccccatg atgcgtcccg atacatcgac caccatgatg accgagaatt tggagtaggg
      301 gcgcgccggg agcgcgaact tctcgcgcag gtccatcacc gggaccgcga tacctttgag
      361 gtcaagcact cccttgacga acgagggggc ggaggggatg cgggtcaggt cccggtagtt
      421 ggttatctcg tgtatcttga ggatgtcggt ggcatagatc tcgtcgccga cgaagaacga
      481 tatgaactgg agcggctgtt cggagcgggc ggcggtgtct tttttcagga atctgctcga
      541 gagattcatg cgacgacctc cgaagtgcgg tgtccgccga aagcgaggtg ctcgataccg
      601 tggatgtcga ggatgatgga aacgcggccg tcgccgagta tggtggcgcc cgatactcct
      661 tcgacgcggc ggaagttggt ctcaagactc tttatgaccg cctgatgctg gccgatgaca
      721 tcgtccacca taagcgccat gcggcgtatg cccgattcga ggattatgac gatggcctct
      781 tccgggttcg tgatggcgtc atcgaagccc agtatctcgt aaagccggat gagcgggagg
      841 tattcgcccc ggaactctat gagttctccc ttgccctcga tggtcttgat gccgtcgggg
      901 gcggggcgga tcgcctcgat gatcgagagg agcgggatgg tgagtatctc ggatccgacc
      961 gcgacgcgca tgccgtcgat gatggcgagc gtgagcggca gtttgagccg gaacagggtg
     1021 ccgcttcctt tctcggagta gacctctatg gcgccgcgca ggt
//
LOCUS       DAOQUE010000685         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_208160_length_1061_cov_4.493042, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000685 DAOQUE010000000
VERSION     DAOQUE010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208160_length_1061_cov_4.493042"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKH10_14140"
     CDS             <1..324
                     /locus_tag="PKH10_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNT29306.1"
                     /translation="VGTAGDFIEVLIEKNEPLPIERTSIFTNASDNQRTVRVGVYQGD
                     RTRKSESHLMGEILLADLRAAKRGELKIQVTFEVNTDGVLNVRAMDLETGTQQHIELN
                     ILGLG"
     gene            329..>1061
                     /locus_tag="PKH10_14145"
     CDS             329..>1061
                     /locus_tag="PKH10_14145"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNT29307.1"
                     /translation="MSYRIGIYLKDEKYLSALSGFLAQKLPDTEVFPFRSLKEFSTVS
                     DGDLRVVIISTLLNEGLWLRAMPALKKAPHILLIAFPDGPEITDDIAHKYGADKLFRA
                     PLSSNDLLASIQQILAADEKKEDESQLPQETVESMQDLFHQIDALDYYQLFNVTADTD
                     QDQIKKIYLDLARRHHPDKFRNAPPEVRRIAYEITKRVNEAYSVLSHPNRRKMYEKMK
                     RENPELKRFDFRLKMRYEENPHDTIH"
BASE COUNT          256 a          348 c          295 g          162 t
ORIGIN      
        1 gtcggcaccg ccggcgactt catcgaggtg ctcatcgaga agaacgagcc gctccccatc
       61 gaacggacga gcatcttcac caacgcctcg gacaaccagc ggacggtccg cgtcggcgtc
      121 tatcagggcg atcgcacccg gaagagcgag tcgcacctga tgggcgagat actccttgcc
      181 gacctccgcg ccgccaaacg gggcgaactg aagatacagg tgaccttcga agtgaacacg
      241 gacggcgtcc tgaatgtccg ggcgatggac ctcgaaacgg gcacccagca acacatagaa
      301 ctgaacatcc ttggcttggg gtgatatcat gagttatcgc atcggcatct acctgaagga
      361 cgaaaagtac ctctcggcgc tgagcgggtt cctcgcgcag aaattgcccg acacggaggt
      421 gttccccttc cgcagcctga aagagttcag caccgtgagc gacggcgacc tgcgggtggt
      481 gatcatcagc acgctcctca acgaggggct ctggctccgc gccatgccgg cgctcaagaa
      541 ggcgccgcac atactgctca tcgcgttccc ggacggaccg gagataaccg acgacatcgc
      601 ccataaatac ggcgccgaca aactgttccg ggccccgctt tccagcaacg acctgctcgc
      661 ctctatccag cagatactcg ccgcggacga aaagaaagag gacgagtcgc aactcccgca
      721 ggagaccgtc gaatctatgc aggacctgtt ccatcagatc gacgcgctcg actactacca
      781 actgttcaat gtcaccgccg acaccgatca ggaccagata aagaagatct atctggacct
      841 cgcccgccgc catcaccccg acaagttccg caacgcgccg ccggaggtgc gccggatcgc
      901 ctacgagata accaaacggg tgaacgaggc ctactcggtc ctgtcgcatc cgaaccgccg
      961 caagatgtac gagaagatga aacgggagaa ccccgaactc aagcggttcg acttccggct
     1021 caagatgcgg tacgaggaga acccgcacga caccatccac g
//
LOCUS       DAOQUE010000686         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_213254_length_1046_cov_3.338042, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000686 DAOQUE010000000
VERSION     DAOQUE010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213254_length_1046_cov_3.338042"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..997
                     /gene="atpG"
                     /locus_tag="PKH10_14150"
     CDS             113..997
                     /gene="atpG"
                     /locus_tag="PKH10_14150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNT29308.1"
                     /translation="MATGLKTIRKRIQSVRNTQKITRAMKMVAASKLRRAQAAMLSIR
                     RYSNELRRLAGLIADRVEGVHPLMVPRDTVRRELIVVVSGDRGLCGSFNANIARAVRR
                     RIERDGLRPEEVTLLFIGRKAAESLRDLPVRRETLFMDTLKKLSSEAMTPIIDLLAQG
                     YLRGDHDRVTVIFNRFRSALAQEIEEEQLFPITENIFEPQEIARDAAGRDTLFEPSPA
                     VIADHLVPHFAATELYHYLMESVASELSARMNAMEAATKNASEMTGRLLIAYNKARQN
                     AITTELMEIVGGAEAQSA"
BASE COUNT          222 a          319 c          352 g          153 t
ORIGIN      
        1 cagcgacgcc acggcggccg ctctgcgcga gatgctcgac gcgttcgagg aatatctcga
       61 aaaggaggtc ttcggtgccg cgaaagaggc ggaggccggg gcgtaacagg cgatggcgac
      121 cgggctgaaa accatacgca agcgcatcca gagcgtgcgg aacacccaga agataacccg
      181 cgccatgaag atggtcgcgg cgagtaaact gcgccgcgcg caggccgcga tgctgtcgat
      241 acgccgctat tccaacgaac tgcggcgcct tgcggggctg atcgccgacc gcgtcgaggg
      301 ggtccatccg ctcatggtgc cgcgcgatac ggtgcgccgc gaactcatcg tcgtggtgtc
      361 gggcgaccgg ggtctgtgcg ggtcgttcaa cgccaacatc gcccgcgccg tgcggcgccg
      421 catcgaacgg gacggcctgc gaccggagga ggtgacgctc ctcttcatcg ggcgcaaggc
      481 ggccgagtcg ctccgcgacc tgccggtgcg tcgcgaaacg ctgttcatgg acactttgaa
      541 aaaactgtcc tccgaagcga tgacgccgat catcgacctg ctcgcacagg gctatctgcg
      601 cggcgaccac gaccgtgtca cggtgatatt caaccggttc cgctcggcgc tcgcgcagga
      661 gatagaggag gaacagttgt tcccgatcac cgagaacatc ttcgaaccgc aggagatagc
      721 gcgcgacgcg gcgggacgcg acacgctgtt cgaaccgtcg ccggcggtca tagccgacca
      781 tctcgtgccc catttcgcgg ccaccgaact gtatcactac ctcatggaat cggtggcgag
      841 cgaactgtcg gcccgcatga acgcgatgga ggcggcgacc aagaacgcgt ccgagatgac
      901 cgggcgactg ctcatcgctt acaataaggc gcggcagaac gcgataacga ccgaactcat
      961 ggagatagtc ggcggcgccg aggcgcagtc ggcatgacgg gaaaagaaca cgagagaggg
     1021 agtatacgat gatcgagaaa gggacg
//
LOCUS       DAOQUE010000687         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_216104_length_1038_cov_3.402848, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000687 DAOQUE010000000
VERSION     DAOQUE010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216104_length_1038_cov_3.402848"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PKH10_14155"
     CDS             complement(<1..875)
                     /locus_tag="PKH10_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29309.1"
                     /translation="MARDGERRRGNDTRRRPTTGGVHLVRVTEEAVPEEPGTVRRIFT
                     LSHRSAAGTLAWEKDVTPTDPSVFFGSMAVGPSGEIVLAGLMDDDRFNENGYPLTDVY
                     LARYSAAGDLLWDLQWDGGHDDMPIALAFDADDNVYLLGTTKVATRQGPDTYQFPYPF
                     AKKFAPDGATLWEKRLDFFVGVDFDGGIALGTDGRIHAAGARGHTVFVATFEPDGTEV
                     AVRSIDTQYFDGTVADVAVDPEGYLYVTGITYEHTTMGLEDIYLMKIDPELIAIPPDD
                     DIADDDDATDDDATVP"
     gene            complement(794..>1038)
                     /locus_tag="PKH10_14160"
     CDS             complement(794..>1038)
                     /locus_tag="PKH10_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29310.1"
                     /translation="FLTKWQDDGTKVWTRLWGSTSMDTQYDLAVDATGSIYSLGSGMV
                     AGFDAAGDESWRVTGSAGAGTILAAAPRPAGYIWSG"
BASE COUNT          204 a          351 c          293 g          190 t
ORIGIN      
        1 ggaacggtcg catcatcgtc ggtcgcgtcg tcatcatcgg cgatgtcgtc gtcgggaggg
       61 atcgcgataa gttccggatc gatcttcatc agatagatat cctcaagccc catcgtcgtg
      121 tgttcgtaag tgatcccggt cacatacaag tatccttccg gatcgaccgc cacatcggcg
      181 accgtaccgt cgaaatactg agtatcgatc gatcgtaccg ccacctcggt cccgtccggc
      241 tcaaaggtcg cgacgaacac ggtatgtcct ctcgcgcccg ccgcatggat ccggccgtcg
      301 gtccccagtg cgatgccgcc gtcgaaatcg acgccgacga aaaagtccag ccgcttctcc
      361 cacagcgtcg cgccgtcggg agcgaacttc ttcgcgaacg ggtacgggaa ctgataggta
      421 tcgggtccct gcctggtcgc gaccttggtc gtaccgagca gatacacatt atcatccgca
      481 tcgaaggcga gcgcgatcgg catgtcgtca tggccgccgt cccactgcag gtcccagagc
      541 agatcgcccg cggcggaata ccgggccaga tagacatcgg tcagcgggta gccattctcg
      601 ttgaaacgat catcgtccat gagcccggca aggacgatct cgccggaagg ccccacagcc
      661 atggaaccga agaaaacgga gggatcggtc ggcgtgacat ccttctccca tgccagcgtt
      721 ccggccgccg aacggtgact caacgtgaat atccggcgca ccgtccccgg ctcttcgggg
      781 accgcctcct ccgtcacccg gaccagatgt accccgccgg tcgtggggcg gcggcgagta
      841 tcgttcccgc gccggcgctc cccgtcacgc gccacgactc atcgccggcg gcatcgaatc
      901 cggccaccat gcccgaaccg agggaataga tgcttcccgt cgcatcgacc gcgagatcgt
      961 attgggtatc catagaggtt gatccccaca accgtgtcca caccttcgta ccgtcatcct
     1021 gccatttggt caagaaca
//
LOCUS       DAOQUE010000688         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_216161_length_1038_cov_2.698881, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000688 DAOQUE010000000
VERSION     DAOQUE010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216161_length_1038_cov_2.698881"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1038)
                     /locus_tag="PKH10_14165"
     CDS             complement(<1..>1038)
                     /locus_tag="PKH10_14165"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNT29311.1"
                     /translation="SAFYRIVGAHPVDVTAFGTTGSGRKLVGEVFGADTIVNEITAHG
                     TGAAFFYPDAETIFEIGGQDAKYIRLEDGLVADVNMNYVCAAGTGSFVEEQARKLGYT
                     LDRIGPVTEHLAPPVTSDRCTVFMEQDLRAILKQGYSKQEALAAVLYSVIQNYLTKVV
                     GNRPVNRAKVYFQGATARNKGLVAAIENLLGVEVVVSPFCHVMGSIGAAQVAIEKTEG
                     AASRFIGPQAATVTVTTGTDICKLCTNYCRINTITRDGGGSFSWGYMCGRDPSEEKRR
                     ELPEYEAFRSRNAQFYRKETAIAPAKKKGVVHLPHTLVNHTYYPLWRRFFALLGYETQ
                     LSALYSTTPIR"
BASE COUNT          168 a          334 c          334 g          202 t
ORIGIN      
        1 tcgcggatgg gcgtcgtgct gtagagcgcg gagagttgag tctcgtagcc gagaagcgcg
       61 aagaaccgcc gccacagcgg gtagtaggtg tggttgacga gcgtatgcgg cagatggacg
      121 acccccttct tcttcgccgg agcgatcgcc gtctccttgc ggtagaactg cgcgttacgg
      181 gagcggaacg cctcgtactc cggcagttcg cgccgttttt cctccgacgg gtcgcggccg
      241 cacatgtagc cccacgagaa acttccgccg ccgtcgcggg tgatggtgtt gatgcggcaa
      301 tagttggtgc agagtttgca gatatcggtg ccggtcgtca cggtcaccgt ggcggcctgc
      361 ggcccgatga agcggctcgc cgccccttcg gtcttctcga tcgcgacctg cgccgcgccg
      421 atgctcccca tcacatggca gaacggcgac acgaccacct cgaccccgag caggttctcg
      481 atggcggcca cgagcccctt gttgcgcgcc gtcgccccct gaaaatagac cttcgcgcgg
      541 ttcaccggcc ggttgccgac caccttggtg agatagttct ggatgaccga atagagcact
      601 gccgcgagcg cctcctgctt gctgtagccc tgcttgagga tggcgcggag gtcctgttcc
      661 atgaagacgg tgcatcgatc gctcgtcacc ggcggtgcga gatgttcggt caccggcccg
      721 atgcggtcga gcgtatagcc gagtttccgg gcctgttcct cgacgaaact gccggtaccg
      781 gcggcgcaga catagttcat gttgacatcg gccaccagcc cgtcctcgag ccggatgtac
      841 ttcgcgtcct gtccgccgat ctcgaatatc gtctcggcgt cgggatagaa gaaagcggcc
      901 ccggtgccat gcgccgttat ctcgttgact atcgtgtcgg ccccgaacac ctcgccgacc
      961 agtttgcgcc cgctcccggt cgtgccgaac gccgtgacat caacgggatg ggcgccgacg
     1021 atgcggtaga aggcactg
//
LOCUS       DAOQUE010000689         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_218557_length_1031_cov_4.330943, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000689 DAOQUE010000000
VERSION     DAOQUE010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218557_length_1031_cov_4.330943"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1031
                     /locus_tag="PKH10_14170"
     CDS             <1..>1031
                     /locus_tag="PKH10_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNT29312.1"
                     /translation="GALTTTYTASQGLLLMIPNLYKIAAELLPGVFHVSARTVATHAL
                     SIFGDHSDVMACRQTGVCLLSSNSVQEAQDLGLVAHLAAIRSSLPFIHFFDGFRTSHE
                     VNKIAMIDYEDIKNIVDHNAIAAFKDRALRPERPVLKGTAQNPDIFFQARETCNPHYD
                     AVPQIVIEEMEKIFAITGRRYKLFDYVGHPEAERIIIAMGSGCEAIDETICHLMEKGE
                     KVGVVKVRLYRPFSVEHFLQALPRTVKAIAVLDRTKEPGSEGEPLYKDVVTALYEQGE
                     DITVVGGRYGLSSKEFTPGMVKAVFDNLKGRSPKHNFTVGITDDISFTSLPYEPLDTV
                     PAGTTQAKF"
BASE COUNT          206 a          359 c          288 g          178 t
ORIGIN      
        1 cggcgcgctc accaccacct acaccgcgtc gcaggggctc ctgctgatga tccccaacct
       61 ttacaaaata gcggctgaac tgttgccggg cgtgttccat gtctcggcgc gcaccgtcgc
      121 gacccacgcc ctgtccatct tcggcgacca cagcgatgtc atggcctgtc gccagaccgg
      181 cgtctgtctg ctgtcatcca actcggtaca ggaggcgcag gacctcggac tcgtcgccca
      241 ccttgcggcc atccggtcga gtctcccgtt catccacttc ttcgacggct tccgcacgag
      301 ccatgaggtc aacaagatcg cgatgatcga ctacgaggac atcaaaaata tcgtcgatca
      361 caatgccatc gccgcgttca aggaccgcgc cctgcgcccc gagcgccccg tactcaaggg
      421 gaccgcgcag aaccccgaca tattcttcca ggcccgcgag acctgtaacc cgcactatga
      481 cgcggtgccg cagatcgtca tcgaggagat ggaaaagatc ttcgcgataa ccggccgccg
      541 ttacaaactg ttcgactatg tcggccatcc cgaggcggaa aggatcatca tcgcgatggg
      601 ttccggctgc gaggcgatcg acgagaccat ctgtcacctg atggaaaagg gcgagaaggt
      661 gggcgtggtc aaggtccgcc tctaccgccc cttctcggtc gaacatttcc tgcaggcgct
      721 gccgcgcacc gtcaaggcga tcgcggtcct cgaccgcacc aaggagccgg gctccgaggg
      781 cgagccgctc tacaaagatg tggtcaccgc gctctacgag cagggcgagg acatcacggt
      841 ggtcggcggt cgctacggac tctcctcgaa ggaattcacc cccggcatgg tcaaggcggt
      901 cttcgacaac ctcaagggcc gctcgccgaa gcacaacttc accgtcggca tcaccgacga
      961 catatcgttc acctcgctgc cctacgagcc gctcgacacc gtcccggccg gcaccacgca
     1021 ggcgaagttc t
//
LOCUS       DAOQUE010000690         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_218954_length_1030_cov_4.509744, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000690 DAOQUE010000000
VERSION     DAOQUE010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218954_length_1030_cov_4.509744"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PKH10_14175"
     CDS             <1..866
                     /locus_tag="PKH10_14175"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT29313.1"
                     /translation="LAVAQVRFNLTVHGYCLMGNHYHLLVGTPDANLSRAMHTLNSSY
                     ASWYRARHQLVGSLFQGRFKSVLVEEERYLAEVSAYIHLNPVRAGLVNSPEAWKWSSM
                     GHYLGKKPPAKKNELTLAVTTDEVLRLCGGRKNYLAIIDEVWARKPDAESIYGANSLL
                     GDEDFVDEMKMRLRAAKEGRVEHMPEAKDILRNDPERVERAVRAALGLGKETTIARRR
                     GDTGYRLMVWALRRHSALRLSEIGARFGIKASAVSELVRHTDEMALSDKDMRKTIADI
                     ATILKRPETVK"
BASE COUNT          216 a          286 c          337 g          191 t
ORIGIN      
        1 gcctcgccgt ggcgcaggtg cggttcaacc tcaccgtgca tggctactgc ctcatgggga
       61 accattacca cctgctcgtc ggtacccccg acgccaatct ctcgcgggcg atgcacaccc
      121 tcaactcaag ttacgcctcg tggtaccgcg cgcggcatca actggtcggg tcgctgttcc
      181 aaggccgctt caaatcggtg ctcgtcgagg aggaacggta tctcgccgag gtgtcggcct
      241 acatccacct caacccggtg cgcgccggac tggtgaacag ccccgaagca tggaaatggt
      301 cgagcatggg gcattatctg ggtaagaagc cgcccgcgaa gaagaacgaa ctgacgctcg
      361 cggtgaccac cgacgaggtg ttgcggctgt gcggcgggcg gaagaactat ctcgccatca
      421 tcgacgaggt gtgggcgaga aagcccgacg ccgagagcat ctacggggcg aactctctgc
      481 tgggcgacga ggatttcgtg gacgagatga agatgcgtct gcgggcggcg aaggaggggc
      541 gggtggagca catgcccgag gcgaaagata tcctgcggaa cgacccggaa cgggtcgagc
      601 gggcggtgcg ggcggcgttg ggactcggca aagagacgac catcgcgcgc agaaggggag
      661 atacgggtta ccggctcatg gtatgggcgc ttcgacggca cagcgcactg cggttaagcg
      721 agatcggtgc gcgattcggt atcaaggcga gcgccgtatc ggaattggtg cgccacaccg
      781 acgagatggc cttatccgat aaggatatgc gaaagacgat agccgacatt gcgacgatat
      841 tgaagaggcc agaaaccgta aaatgaagga ctgacccctc tatcttgcat ttcggtccga
      901 ggcgtgtatc atggcccgtt cccgcgctgt gttgaggagg cgttgcccga agtgagtaaa
      961 ctcgccgaat tcgttataaa atatcgctgg ggcatcattt tttcccatgt cgccctttcg
     1021 cttcttttcc
//
LOCUS       DAOQUE010000691         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_220522_length_1026_cov_2.373841, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000691 DAOQUE010000000
VERSION     DAOQUE010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220522_length_1026_cov_2.373841"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1026)
                     /locus_tag="PKH10_14180"
     CDS             complement(<1..>1026)
                     /locus_tag="PKH10_14180"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HNT29314.1"
                     /translation="VADPGDPCDDTLFCNGADHCSGGECALHDDDPCDALETCVEGRG
                     CLTECETAEAKESYVGCEYWGAFLQNDTPNNYAVVVANPNDTEVTVTAYGNGNVELAQ
                     FTVAAYGVNSQTFDTTRHLTGAGIFDHAFKFVASRPVTMTQMNPFGNVLTYTNDASLL
                     LPKGALGHQYYAMSWPDWYYYEGSGCEGEPVTTYNYPGFVSVVAVEPGTTTVTVTYHG
                     DSAPGDGEFAAQEAGDTVQYTLTQYQILNLNSAAGSGTVTAYGADLTGSLIEADKRVA
                     VFGGHECTDVPADKFACDHLEHQVFPLRSWGKEYVAVRTKSCGTESDYLRILASVNGT
                     SVSWSGG"
BASE COUNT          166 a          314 c          345 g          201 t
ORIGIN      
        1 atgccgccgg accacgagac gctggtgccg ttcaccgagg cgaggatgcg gagatagtcc
       61 gattcggtgc cgcacgactt ggtgcggacc gcaacatact ctttacccca cgaacggagc
      121 gggaagacct gatgttcgag atggtcgcag gcgaatttgt cggcggggac atcggtgcat
      181 tcgtggccgc cgaagaccgc cactctcttg tcggcctcta tcagactgcc cgtcaggtcg
      241 gcgccgtacg cggtgacggt gccgctcccc gcggccgagt tgaggttgag tatctggtac
      301 tgggtgagcg tgtactgcac cgtgtcgcct gcttcctgtg ccgcgaactc gccgtcgccg
      361 ggggccgaat cgccgtggta ggtgaccgtc accgtcgtcg tccccggctc gacggcgacc
      421 accgagacga agccggggta gttgtaggtg gtcacgggct cgccttcgca gccgcttccc
      481 tcgtagtaat accagtcggg ccaactcatc gcgtaatact ggtgccccaa cgcccccttg
      541 ggcaggagga gcgacgcgtc gttggtatag gtcagcacat tgccgaacgg gttcatctgg
      601 gtcatggtga ccggtcgcga cgcgacgaat ttgaaagcgt ggtcgaatat cccggcgccg
      661 gtcaggtggc gggtggtgtc gaaggtctga gaattgacac cgtacgccgc cacggtgaac
      721 tgggcgagtt cgacatttcc gttcccgtag gccgttaccg tcacctcggt gtcgttcggg
      781 ttggcgacca cgacggcgta gttgttgggg gtgtcgttct gcaggaaggc gccccagtat
      841 tcacagccga cataactttc cttcgcctcg gcggtctcgc actcggtcag acagccgcgc
      901 ccctcgacgc aggtctcaag cgcgtcgcag ggatcgtcat cgtgcagggc gcattcgccg
      961 ccgctacagt ggtcggcgcc gttgcagaac agcgtatcgt cgcagggatc gccggggtcg
     1021 gcgacc
//
LOCUS       DAOQUE010000692         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_227320_length_1008_cov_2.598111, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000692 DAOQUE010000000
VERSION     DAOQUE010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_227320_length_1008_cov_2.598111"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..>1008)
                     /locus_tag="PKH10_14185"
     CDS             complement(121..>1008)
                     /locus_tag="PKH10_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29315.1"
                     /translation="PTPPEPPVATDPLDDDEYAPDESAYDKIVEAVKRRDYPYLSKEL
                     LGIETKKLTTELAHALIKRIADAYELDTRATNLAELAPLFVDIVPDDYREVHNALLKQ
                     VWEMNSFFYSGRILSAVVELLIARGEYDWALNFAKRFRDRFETDEAVAETLLYGLDDF
                     DESLITEEYHSSTENRMRPERCRERIGERVYEAGDKGKALEYFKDALGEAFAAGGVDP
                     AKIVLAMTMHFLARHGDGETAARIREFLETAAKDKTVLHITVGDGIGGGIDRLDAGYF
                     TALFDWLQSHLGEKYHARP"
BASE COUNT          193 a          270 c          310 g          235 t
ORIGIN      
        1 acggtgatga aaagtttttg ttgctcttct gcaagacaag gaatggagaa agaaagaagc
       61 acaagaataa ggagaatgtt tctcatggca gatccctcca agaaagtttt tctattacat
      121 ttacgggcgg gcatgatatt tctccccgag gtgtgattgg agccaatcga aaagggcggt
      181 gaaatagccc gcatccagac ggtcgatacc gccgccgata ccgtcgccga cggtgatgtg
      241 gagaaccgtc ttgtccttcg cggccgtttc caaaaattcg cgtatccgcg ccgccgtttc
      301 accgtcgccg tgacgggcga gaaagtgcat cgtcatggcc agaacgatct tcgccgggtc
      361 gacgccgccg gccgcgaacg cttcgccgag cgcgtccttg aaatactcca gcgcctttcc
      421 tttgtcgccc gcttcgtaca cccgttcgcc gatccgttca cggcaccgtt cggggcgcat
      481 ccggttttcg gtcgatgagt gatactcttc ggtgatgagg ctttcgtcga aatcgtccag
      541 cccgtacaac agcgtttcgg ccaccgcttc gtcggtctcg aaacgatccc ggaaccgttt
      601 ggcgaaattg agcgcccagt cgtattcgcc ccgtgcgatc agcagttcga cgaccgccga
      661 aaggatccgc ccggaataaa agaaactgtt catttcccag acctgtttga gcagggcgtt
      721 atggacttcg cggtagtcgt cggggacgat atccacgaag agcggggcga gttccgcgag
      781 gttggtagcc cgcgtgtcga gttcgtatgc gtcggcgatc cgtttgatca gggcgtgggc
      841 gagttccgtg gtgagttttt tcgtttcgat gccgaggagt tctttggaaa ggtaggggta
      901 atcgcgccgt ttgaccgctt cgactatctt gtcgtaggcg ctttcgtccg gggcgtactc
      961 gtcgtcgtcg agcggatcgg tggcgacggg cggttcgggc ggggtagg
//
LOCUS       DAOQUE010000693         1001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1122
            NODE_230078_length_1001_cov_4.469345, whole genome shotgun
            sequence.
ACCESSION   DAOQUE010000693 DAOQUE010000000
VERSION     DAOQUE010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852465
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:50:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,802
            Genes (coding)                    :: 2,784
            CDSs (with protein)               :: 2,784
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230078_length_1001_cov_4.469345"
                     /isolate="AMR_MDS_1122"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PKH10_14190"
     CDS             <1..826
                     /locus_tag="PKH10_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT29316.1"
                     /translation="APVNLYNPFSAGVSDSFALLLGGVLGGMAAQASIQPIFAAKDVS
                     TAKRASVLAGFIVAPFGIMTAFIALYAKTGLFFDAAQVTDAKTVLTRILTTPEFIPPV
                     LGGLALAGALAAILSTVGPVNFAVVTIAAKDIYHGMINKEADDKKIIATARKLVVLVG
                     IVTIPLAIFLRGAVLDTAYVSYAIRAIGAIVILTGIYARGWISARGVRLAFILGTVVI
                     FGTILAKEMGWFSVDKTYGAVIATVTILVIVKLYEKITGAQDDPRDRAKNTEEHTA"
BASE COUNT          177 a          347 c          291 g          186 t
ORIGIN      
        1 cgcgccggtc aatctgtaca atcccttcag cgccggggtc tccgactcct tcgccctcct
       61 gctcggcggc gtactcggcg gcatggcggc gcaggcgagc atccagccga tattcgccgc
      121 gaaggatgtc tcgaccgcca aacgcgcctc ggtgctcgcc ggcttcatcg tcgccccctt
      181 cggcatcatg accgccttca tcgccctcta cgcgaaaacg ggtcttttct tcgatgccgc
      241 gcaggtgacc gacgccaaga cggtcctgac ccgcatcctc accacgcccg aattcatccc
      301 gccggtgctc ggcggtctgg cgctcgccgg ggcgctggcg gcgatacttt cgaccgtcgg
      361 accggtcaat ttcgccgtgg tgaccatcgc cgcgaaggat atctatcacg gcatgatcaa
      421 taaggaggcc gatgacaaga agatcatcgc gacggcccgg aaactggtgg tgctcgtcgg
      481 catcgtcacg ataccgctcg ccatcttcct gcgcggcgcg gtgctcgaca ccgcctatgt
      541 ctcctacgcc atccgggcga tcggcgccat cgtcatcctg acgggcatct acgcccgcgg
      601 ctggatatcg gcgcgcggcg tgcggctcgc cttcatactc ggcacggtcg tcatcttcgg
      661 caccattctc gccaaggaga tgggctggtt cagcgtcgac aagacctacg gcgcggtcat
      721 cgccaccgtg acgatactgg ttatcgtcaa actctacgaa aagatcaccg gagcgcagga
      781 cgatccgcgc gacagggcga aaaacacgga ggagcatacg gcatgagcga acgctacttc
      841 atcgacaatt cgcgcctcat catcgaggcg acggcgcggg ccggggccga tgtcttcatc
      901 ggctacccga taaccccggc gaacctgctg tatctgtacg cttcgcagcg gttcccggtc
      961 gtcctgcctg ctcccgacga gataacgacg ctccagtgga t
//
